This is a Macintosh program for drawing gene maps. It requires the data also in this directory, or else data in these formats. The files are: flybase/clones/ucla.genemap/newkb.txt flybase/clones/ucla.genemap/genedraw.data flybase/clones/ucla.genemap/genedraw.readme flybase/clones/ucla.genemap/genefix.txt flybase/clones/ucla.genemap/kb4band.txt flybase/clones/ucla.genemap/genedraw.hqx How To Make Your Own Map Of Clones Introduction Genetic information about a chromosome location (eg clones, deletions or inversions, transcript units) is often easier to comprehend from a graph than a table. The Genedraw program presented here converts the clone list table to a graph showing clone locations along the salivary gland chromosome map. Because distances between polytene locations are uneven due to band sizes and compaction, we wrote the program to "stretch out" the chromosome by incorporating the DNA length estimates of the individual bands. These data were kindly supplied by V. Sorsa (Chromosome Maps of Drosophila, CRC Press, 1988). Genedraw was written in Think C to preserve the Mac display functions. Implementation Outline 1. How to down-load the genedraw program and data files 2. How to decompress the genedraw program 3. How to execute the genedraw program with data 1. How to down-load the genedraw program and data files When you double-click ucla.genemap folder, you will see the following files. genedraw.readme genedraw genedraw.data genefix kb4band newkb Now, double-click genedraw.data, and it will copy the file onto your MacIntosh. The Gopher may say the file is too long, but don't worry, the whole content will be copied. double-click genefix and the window will pop up showing the content of genefix. Move your cursor to File menu and choose Save a Copy. Then it will ask you in what name you want to save the file. Type genefix.txt. (This is important: the name of this file on your Mac should be genefix.txt.) Repeat the same step for kb4band and newkb. (Again, it is extremely important that these files should be saved as kb4band.txt and newkb.txt) Finally, double-click genedraw. Then it will ask you in what name you are going to save this. Answer genedraw.sit. Then in most cases, the program called StuffIt will automatically run, popping up a window showing genedraw.exe highlighted in it. (As you may notice from the icon, genedraw is a Macintosh BINHEX file(compressed). It means we have to do an extra work to decompress it). Detailed steps of decompression are continued in the next section. 2. How to decompress the genedraw program Continuing from the above section, there can be 2 possibilities. Either StuffIt is automatically activated or not. But, in either case, you should have StuffIt program on your Macintosh. If StuffIt is executed automatically, a window will pop up showing genedraw.exe highlighted. Just double-click it, and it will ask you again in what name you want to save it. Answer any name you like(preferably genedraw.exe). Then you will see a new icon or a file name of genedraw.exe. If StuffIt does not run automatically, you decompress the file genedraw.sit using StuffIt manually. Open the file which is created by saving the genedraw from Gopher. Move your mouse to Other of the menu and select it, Then choose Decode BinHex File..... Confirm if the decompressed file is created. 3. How to execute the genedraw program with data Running genedraw is simple. Just double-click the icon or file name of the decompressed genedraw file(in most cases, genedraw.exe). But, one important thing is that you must have newkb.txt and kb4band.txt and genedraw.data in the same folder. Especially, confirm the name of newkb.txt and kb4band.txt. If even one file is missing or saved in other names, genedraw can not work. The scroll bar on the screen bottom lets you change the chromosome section you want to display. This version of Genedraw gives you a choice from a pull down menu of either one or two numbered sections per screen. Comments and questions please to JMERRIAM@cognet.UCLA.edu.