LOCUS VJ-03 (pLexA-ChR 9724 bp DNA circular 21-DEC-2011 SOURCE ORGANISM COMMENT VNTDATE|590840379| COMMENT VNTDBDATE|590840379| COMMENT VNTNAME|VJ-03 (pLexA-ChR2 ET/TC-Venus)| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS 3465..4082 /dnas_title="w+" /vntifkey="4" /label=w+ rep_origin 1151..1770 /dnas_title="pBR322 Ori" /vntifkey="33" /label=pBR322 Ori polyA_signal 9210..9832 /dnas_title="Sv-40 polyA" /vntifkey="25" /label=Sv-40 polyA enhancer 6742..6857 /dnas_title="5x LexAop" /vntifkey="9" /label=5x LexAop enhancer 6500..6615 /dnas_title="5xLexAop" /vntifkey="9" /label=5xLexAop enhancer 6653..6714 /dnas_title="3x LexAop" /vntifkey="9" /label=3x LexAop intron 7143..7209 /dnas_title="myosin IVS" /vntifkey="15" /label=myosin IVS promoter 6888..7115 /dnas_title="HS promoter" /vntifkey="29" /label=HS promoter CDS 136..996 /dnas_title="Ampicillin" /vntifkey="4" /label=Ampicillin promoter 66..94 /dnas_title="Amp promoter" /vntifkey="30" /label=Amp promoter misc_feature 7226..7229 /note="Cavener sequence" CDS 7230..8264 /label=Chrimson CDS 8370..9086 /dnas_title="mVenus no ATG *D.M." /vntifkey="4" /label=mVenus no ATG *D.M. CDS 8283..8369 /dnas_title="linker" /vntifkey="4" /label=linker misc_feature 8292..8348 /dnas_title="Kir2 traffic signal" /vntifkey="21" /label=Kir2 traffic signal misc_feature 9087..9107 /dnas_title="ER export" /vntifkey="21" /label=ER export ORIGIN 1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6481 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6541 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6601 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6661 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6721 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6781 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6841 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6901 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6961 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7021 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7081 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7141 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7201 ttacttcagg cggccgcggc tcgagcaaaA TGAGCAGACT GGTCGCCGCT TCTTGGCTGC 7261 TGGCTCTCCT CCTCTGCGGA ATTACCAGCA CAACAACAGC CTCTAGCGCC CCAGCAGCTT 7321 CTTCTACAGA CGGAACAGCC GCCGCAGCAG TGTCTCACTA CGCCATGAAC GGCTTCGACG 7381 AGCTGGCTAA AGGAGCCGTG GTGCCAGAAG ACCACTTTGT CTGCGGACCA GCCGACAAGT 7441 GCTATTGCTC CGCTTGGCTG CACAGCAGAG GCACACCAGG AGAAAAGATC GGCGCCCAGG 7501 TCTGCCAGTG GATTGCTTTC AGCATCGCCA TCGCCCTGCT GACATTCTAC GGCTTCAGCG 7561 CCTGGAAGGC CACTTGCGGT TGGGAGGAGG TCTACGTCTG TTGCGTCGAG GTGCTGTTCG 7621 TGACCCTGGA GATCTTCAAG GAGTTCAGCA GCCCCGCCAC AGTGTACCTG TCTACCGGCA 7681 ACCACGCCTA TTGCCTGCGC TACTTCGAGT GGCTGCTGTC TTGCCCCGTG ATCCTGATCA 7741 AGCTGAGCAA CCTGAGCGGC CTGAAGAACG ACTACAGCAA GCGGACCATG GGCCTGATCG 7801 TGTCTTGCGT GGGAATGATC GTGTTCGGCA TGGCCGCAGG ACTGGCTACC GATTGGCTCA 7861 AGTGGCTGCT GTATATCGTG TCTTGCATCT ACGGCGGCTA CATGTACTTC CAGGCCGCCA 7921 AGTGCTACGT GGAAGCCAAC CACAGCGTGC CTAAAGGCCA TTGCCGCATG GTCGTGAAGC 7981 TGATGGCCTA CGCTTACTTC GCCTCTTGGG GCAGCTACCC AATCCTCTGG GCAGTGGGAC 8041 CAGAAGGACT GCTGAAGCTG AGCCCTTACG CCAACAGCAT CGGCCACAGC ATCTGCGACA 8101 TCATCGCCAA GGAGTTTTGG ACCTTCCTGG CCCACCACCT GAGGATCAAG ATCCACGAGC 8161 ACATCCTGAT CCACGGCGAC ATCCGGAAGA CCACCAAGAT GGAGATCGGA GGCGAGGAGG 8221 TGGAAGTGGA AGAGTTCGTG GAGGAGGAGG ACGAGGACAC AGTGgcgatc gctgctgccg 8281 ccgcggcggc caagagcagg atcaccagcg agggcgagta catccccctg gaccagatcg 8341 acatcaacgg aggttcaggc ggaaccggtg ttagcaaggg cgaagagctg tttacgggcg 8401 ttgtgccaat tttggtggaa ctggatggag acgtcaacgg ccacaagttc agcgtgtccg 8461 gtgagggcga aggagatgcc acctacggca agctgacatt gaaattgatc tgcaccacgg 8521 gtaagctgcc cgtgccgtgg ccaaccttgg tcacaacgct gggttatggc ttgcagtgct 8581 tcgcccgcta ccccgatcac atgaagcaac atgacttttt caagtccgct atgccggaag 8641 gatatgtcca agagcgcacg attttcttta aggatgacgg caactacaaa acccgcgccg 8701 aggtgaagtt cgaaggcgat accctggtga accgcatcga gttgaaaggc attgacttta 8761 aggaagatgg aaatatactg ggccacaaat tggagtacaa ctataattcg cataatgtct 8821 acatcaccgc cgataagcag aaaaacggca ttaaggcgaa cttcaaaatc cgccacaata 8881 tagaggatgg cggagtccaa ctggccgatc actaccaaca gaacacgccg attggagacg 8941 gccccgtctt gctgccagat aaccactacc tgagctatca gtccaagttg tcgaaagatc 9001 cgaacgagaa acgcgaccac atggtgctgt tggagttcgt gaccgccgct ggcatcacgc 9061 tgggaatgga tgagttgtac aagtaattct gctacgagaa cgaggtgggt accactgtta 9121 attaacctct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac 9181 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt 9241 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa 9301 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca 9361 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga 9421 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg 9481 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg 9541 ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat 9601 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 9661 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 9721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 9781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgatctggc 9841 cggccgttta aacgaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg 9901 ttaatgtcat gataataatg gtttctta //