Suggestions for authors. ~~~~~~~~~~~~~~~~~~~~~~~~ We literature curators stock FlyBase with data recorded from your articles about genes, alleles and transgenes. It is important for you, our users, that the information we gather is filed under the correct gene, allele or transgene construct. Sometimes, we are unsure of the identity of a gene or allele your paper discusses, or which transgene you used and then we must write to you for clarification. This document is to explain what information you need to include to minimize the amount of checking that we need to do. Of course this would not be necessary if everyone used valid FlyBase symbols for all the components in all the Materials and Methods sections of all papers. But as we all know, in life and in literature, things are never as simple as that. Different groups publish genes and alleles under a wide variety of names and symbols. This is unlikely ever to change - and it is not the job of FlyBase to seek to change this. However, if we are to maintain the links between all of the information we curate from your papers and the genes or alleles they apply to - a task we hope you agree is a useful one - then we need to know exactly which object you are talking about. For gene symbols where you choose to use an "invalid" symbol, we like it if you state somewhere (the Materials and Methods section is ideal) which FlyBase valid gene symbol or CG genome annotation symbol each gene corresponds to. e.g: "hid (CG5123)" or "hid (FlyBase symbol:W)". Each object in FlyBase has a unique identifier number, and these can also be used for making the identity of items explicit e.g. "hid (FBgn0003997)". For alleles, an example would be "ck[IIQ106] (FlyBase symbol:ck[15])" or "ck[IIQ106] (FBal0001696)". If you were to publish a paper about a newly named gene with molecular information that indicates that you know which CG your "new" gene corresponds to, but do not make a statement about that correspondence, then we will mail you to determine that link. It saves time for everybody if these correspondences are stated up front. FlyBase gives explicit symbols to some objects that the community does not treat with the same nomenclatural respect that it gives to genes and alleles, notably Transgene constructs and Transposable element insertions. You should use the FlyBase symbol or ID number to convey the construct you used e.g. "UAS-TNT (FlyBase symbol:P{UAS-TeTxLC.tnt})" or "UAS-TNT (FBtp0001264)". It can be particularly difficult for us to decide, on the basis of Materials and Methods sections, whether a construct is "new" or has been previously published. Accurately referencing constructs that are not novel secures you a warm place in the hearts of the literature curators. For new transgene constructs the name of the transformation vector used (e.g. pUAST), the selectable marker used and the relationship to previously published constructs are particularly useful pieces of information for us. When describing insertions, for example of enhancer trap elements, the identity of the transgene construct is important. For example "a previously unpublished insertion of P{lArB} into 27C" is much more informative than "an enhancer trap insertion on the second chromosome". You need only tell us the FlyBase symbol which represents each object you discuss once in each paper. You may prefer to use nicknames rather than FlyBase nomenclature throughout the rest of your paper, but if you have followed our guidelines clarity will have been attained. Any name used by you that is not a FlyBase valid symbol is stored as a synonym, so that anyone searching FlyBase using your symbol will find the FlyBase object they are looking for. An aside concerning sequence-level data: FlyBase is trying to map many data types to the genomic sequence. For such data, it would help immeasurably if you would identify an unambiguous sequence reference point or feature to which other sequence coordinates can be accurately related. This can simply be a small stretch of unique sequence 10-20bp long; transcription and translation start sites do not qualify as 'unambiguous'! We hope you find this useful. If you would like to follow these guidelines but are unsure about which symbol/identifier number corresponds to your gene, allele or construct, we would be delighted to help you figure it out as you put your manuscript together. Just write to us at flybase-help@morgan.harvard.edu. We can also help with naming new things. Our nomenclature document gives general guidelines but we are always happy to give specific advice for your particular genes/alleles/constructs. Best regards, the FlyBase literature curators.