*U FBrf0075164 *a Laverty *b T. *t 1995 *T personal communication to FlyBase *u *F From todd_laverty@maillink.berkeley.edu Fri Jan 20 17:46:17 1995 *F Date: 20 Jan 1995 09:45:44 -0800 *F From: Todd Laverty *F Subject: 6E7 *F To: Michael Ashburner *F X-Mailer: Mail*Link SMTP-QM 3.0.1 *F Content-Length: 213 *F Dear Michael, *F *F I repeated the in situ on l(3)j6E7. The result is 87B10-13. Take care. *F *F Todd *F Rubin lab # *U FBrf0075169 *a Nothiger *b R. *t 1994 *T personal communication to FlyBase *u *F From rolnot@zool.unizh.ch Fri Dec 23 15:53:25 1994 *F Date: Fri, 23 Dec 94 16:52:40 +0100 *F To: ma11 *F From: rolnot@zool.unizh.ch (Rolf Nothiger) *F X-Sender: rolnot@rzu-mailhost.unizh.ch *F Subject: Re: Help FlyBase *F Content-Length: 3035 *F Dear Michael, *F I am sorry for the delay in answering your request for FlyBase. Here follow *F your messages, with my corrections/changes in CAPITALS. They mainly *F concern the authorship, and an additional information for Df(2R)bw-S46 *F which also eliminates vir : *F There are three genes in FlyBase for which the only 'reference' is: *F *F \*x Nothiger, 1992, personal communication (Crete meeting) *F The data are: *F \*a Mst59Da *F \*z FBgn0005651 *F \*e Male-specific-transcript-59Da *F \*H Last updated 14 Nov 94 *F \*b 2-[103] *F \*c 59D3--59D4 *F \*x HUANG, SCHNEITER AND Nothiger, 1992, personal communication (Crete meeting) *F \*d male-specific-RNA *F \# *F *F \*a Mst59Db *F \*z FBgn0005652 *F \*e Male-specific-transcript-59Db *F \*H Last updated 14 Nov 94 *F \*b 2-[103] *F \*c 59D3--59D4 *F \*x HUANG, SCHNEITER AND Nothiger, 1992, personal communication (Crete meeting) *F \*d male-specific-RNA *F \# *F *F \*a Mst59Dc *F \*z FBgn0005653 *F \*e Male-specific-transcript-59Dc *F \*H Last updated 14 Nov 94 *F \*b 2-[103] *F \*c 59D3--59D4 *F \*x HUANG, SCHNEITER AND Nothiger, 1992, personal communication (Crete meeting) *F \*d male-specific-RNA *F \# *F *F As part of our policy to document everything it would be good if you *F could confirm that these data are correct & that you are happy for *F them to be in FlyBase (or not so). If you want to add anything, do so. *F Yr answer will then be archived as a FlyBase Personal Communication which *F any user can then look yp. *F Sorry *F *F I should have added these aberrations to that list: *F *F \*a Df(2R)bw-HB132 *F \*x AMREIN, HILFIKER AND Nothiger, 1990, personal communication, Crete meeting *F \*c Df *F \*G vir << bk1 << bw << bk2 *F \# *F \*a Df(2R)bw-S46 *F \*x FBrf0066905 == Lindsley and Zimm, 1992 *F \*x AMREIN, HILFIKER AND Nothiger, personal communication, 1990 Crete meeting *F \*x FBrf0039465 == Simpson, 1983, Genetics 105: 615--632 *F \*B 59D8-59D11;60A7 *F \*C Df *F \*W Richards. *F \*o X ray *F \*G bk1 << Mst59Da << bw << bk2 *F \*q Deficient for bw AND VIR but not twi. *F THAT'S IT. THE REST IS OKAY. *F *F Rolf *F \***** *F Rolf Nothiger, Zool. Inst. UZI, Winterthurerstr. 190, CH-8057 Zurich *F Phone: Switzerland - 1 - 257 48 61 *F Fax: Switzerland - 1 - 361 31 85 *F e-mail: rolnot@zool.unizh.ch *F \***** # *U FBrf0075170 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Fri Dec 30 12:18:17 1994 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 30 Dec 1994 12:14:43 +0000 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: help flybase *F Content-Length: 1298 *F >John *F > *F >FlyBase has this: *F > *F > *F >*a DS(2)TE45FLTE35AR *F >*x G. Ising, personal communication to J. Roote *F > *F > *F >can you trace this, and (best) give me a paper copy of what GI told you (us) *F > *F >M *F Michael, *F I will put a copy of Gunnar's original letter on your desk - good luck! *F In short, he did an experiment suggested by David to make autosynaptics *F from recombination between suitably placed TEs - in this case between TE146 *F and TE1. The cross was Scorv1, net//Scorv1, bw sp males X TE1/TE146 *F females. He picked up a net fly which should be LS(2)Scorv1, net//DS(2)new, *F which he backcrossed to rv1//rv1 and made a stock. The breakpoints of the *F new DS should (if the crossover worked) be 35B;45F, but are in fact *F 35B;44F. *F He doesn't say how easy the expt was e.g. many parents he used. He also *F doesn't say whether the net fly was male or female. *F I have a vague recollection that David was a bit despairing because later *F he got the impression from Ising that he had irradiated the TE1/TE146 *F females before crossing to rv1//rv1. (But I'm not sure - I'll ask DG). *F This new DS was used by Joan Hooper to make the deletions which she called *F Df(2R)R+1 TE, GR15 TE and P14 TE, *F all of which are published in Hooper 1989 Cell 59:751-765. The distal *F breakpoint of these is ~44F. *F HNY *F J # *U FBrf0075171 *a Ising *b G. *t 1985 *T personal communication to FlyBase *u *F Letter from G. Ising to D. Gubb,16 December 1985. *F Copy available from FlyBase # *U FBrf0075172 *a Matthews *b K. *t 1995 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Shigeo Hayashi, National Institute of Genetics, Mishima, Shizuoka-Ken 411, Japan *F To: The Bloomington Drosophila Stock Center *F Subject: Df(3R)3450 *F Dated: unknown *F *F Background: This is the information on an unpublished deficiency that *F accompanied a stock contributed to the Bloomington Stock Center. *F *F Information communicated: *F Df(3R)3450 was isolated by gamma ray irradiation of enhancer trap line 3450 (from Scott/Fuller screen, inserted at *F 98F). The deficiency endpoints are 98E3 and 99A6-8. # *U FBrf0075173 *a Matthews *b K. *t 1995 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Arthur Wohwill, Indiana University *F To: HHMI P-element Stock Center/Bloomington Drosophila Stock Center *F Subject: P82W *F Dated: unknown (to FlyBase 4 Jan 95) *F *F Background: This is the information on two unpublished transposons that accompanied stocks transferred to the Bloomington Stock Center. *F *F Information communicated: *F 33-1 is a lethal insertion of P82W (see map) at 94CD. The P82W vector is based on Casper. *F *F A1 is a lethal insertion of a P-element containing the Drosophila pseudoobscura hsp82 gene in Carnegie 20. This insert is at 85E and was *F mobilized there from a stock originally created by Betsy Jaffe (Charles Laird's lab). *F *F Figure: Map of P82W *F P:5'HSP82:5'w:P:PUC # *U FBrf0075174 *a Saint *b R. *t 1995 *T personal communication to FlyBase *u *F From Robert_Saint@science.ccmail.adelaide.edu.au Thu Jan 5 11:49:51 1995 *F Date: Thu, 05 Jan 1995 21:34 -0600 (CST) *F From: rsaint@biochem.adelaide.edu.au *F Subject: Re[2]: Help FlyBase *F To: ma11@gen.cam.ac.uk *F X-Envelope-To: ma11@gen.cam.ac.uk *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN *F Content-Transfer-Encoding: 7BIT *F Content-Length: 905 *F Michael, *F I am recovering from a short but glorious summer break and can't remember *F whether I replied to this message or not. It can't hurt to reply twice, in any *F case. The entry is correct in that we have sequence from cDNA clones *F confirming it as a new homeobox gene, although I haven't checked the sequence *F databases for some time. The only additional information that I can give is *F the cytological location: 57B. I think I mentioned in the last message that we *F haven't published it, nor are we likely to in the near future, unfortunately. *F Thanks and best wishes, *F Rob. *F Subject: Help FlyBase *F Author: ma11@gen.cam.ac.uk *F Date: 12/7/94 9:11 AM *F Rob *F FlyBase has this rather enigmatic record; *F \*a wom *F \*z FBgn0005641 *F \*e wombat *F \*H Last updated 14 Nov 94 *F \*x Saint, 1992, personal communication (Crete meeting) *F \*d homeodomain-protein *F \# *F We no longer wish to include any data that we cannot document in hard *F copy or electronic form. Could you confirm/deny these data (or should we *F remove them?). Is it published ? Is there anything that you can add ? *F Your reply will then be archived and referenced from within this gene *F record. *F Many thanks *F Michael # *U FBrf0075175 *a Hekmat-Scafe *b D.S. *t 1995 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Daria Hekmat-Scafe, Yale University *F To: Bloomington Drosophila Stock Center *F Subject: OS-C and OS-D location and expression *F Date: 5 January 1995 *F *F Information communicated: *F *F "I noticed an error [in FlyBase] in information from our lab. OS-C and OS-D (unlike OS-E and OS-F) are NOT thought to be pheromone-binding proteins and are NOT located at 83CD. OS-C is at 84E, while OS-D is at 73F. Their expression *F is specific to a subset of the olfactory system, but their function is unknown." *F # *U FBrf0075176 *a Haynes *b S.R. *t 1995 *T personal communication to FlyBase *u *F From shaynes%lmgvax.dnet@dxi.nih.gov Tue Jan 10 21:39:26 1995 *F Date: Tue, 10 Jan 95 16:38:13 -0500 *F From: shaynes%lmgvax.dnet@dxi.nih.gov *F To: dxi::'ma11@gen.cam.ac.uk'@gen.cam.ac.uk *F Subject: Flybase entry *F Content-Length: 1197 *F Gene symbol : Tsr *F Full name : testis-specific-RRM-protein *F Genetic map position : 3-[54] *F Cytological map position : Placed in 87F by its localization adjacent to Hrb87F *F Function(s) of product : ribonucleoprotein *F RNA-binding-protein *F D. mel. DNA/RNA AC no(s) : U18401 *F Phenotypic information : *F Mutation leads to weak male fertility, without much (or any) effect on *F viability or female fertility. *F Misc. allele information : *F cDNA clone isolated from pupal library. Single transcript (1.7 kb) is seen in *F male third instar larvae, pupae and adults; not detectable by RT-PCR in *F females. Transcript is testis-specific, since it is not detectable in *F males lacking a germ line. *F Protein information : *F Tsr encodes a 46.6 kDa protein that contains two RRM-type RNA binding domains *F at the N-terminus and a unique C-terminal domain. The RRMs are most closely *F related to RRMs of A and B hnRNP proteins of vertebrates and Drosophila. *F Protein is located in cytoplasm of primary spermatocytes and elongating *F spermatids, but is absent from fully elongated spermatids. # *U FBrf0075177 *a Kodjabachian *b L. *t 1995 *T personal communication to FlyBase *u *F From kodja@lgpd.univ-mrs.fr Thu Jan 19 17:34:31 1995 *F From: kodja@lgpd.univ-mrs.fr *F Date: Thu, 19 Jan 95 18:10:13 +0100 *F Reply-To: '' *F To: flybase-help@morgan.harvard.edu *F Subject: Corrections about data on corto gene. *F Content-Length: 612 *F Dear Dr Rindone, *F I would be very indebted to you if you could change in flybase a number of *F informations concerning the gene corto referred under the id number FBgn0010313. *F It concerns the genetic map position which is 3-(47.1) instead of 2-(55.1); the *F cytological map position which is 82E instead of 42A; and the informations about *F the mutant phenotype. Please keep only the miscellaneous information on the *F protein. The previous genetic data were inexact and I will show the right ones *F during the next American Drosophila Conference. *F Sincerely yours. *F Kodjabachian Laurent *F LGPD *F Marseille-Luminy France # *U FBrf0075178 *a Eldon *b E. *c H.J. *d Bellen *t 1992 *T personal communication to FlyBase *u *F From E. Eldon and H. Bellen, to J. Merriam, April 17th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075179 *a Boschert *b U. *c F. *d Sadou *e N. *f Amlaiky *g J. *h Plassat *i R. *j Hen *t 1991 *T personal communication to FlyBase *u *F From U. Boschert, F. Sadou, N. Amlaiky, J. Plassat and R. Hen, to J. Merriam, December 17th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075180 *a Tiong *b S.Y.K. *c D. *d Nash *t 1992 *T personal communication to FlyBase *u *F From S. Tiong and D. Nash, to J. Merriam, April 10th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075181 *a Tripoulas *b N. *c A. *d Shearn *t 1990 *T personal communication to FlyBase *u *F From N. Tripoulas and A. Shearn, to J. Merriam, December 20th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075182 *a Kotarski *b ?. *c ?. *d Derk *e J.E. *f Tessiatore *t 1991 *T personal communication to FlyBase *u *F From Kotarski, Derk and Tessiatore, to J. Merriam, March 18th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075183 *a Howells *b A.J. *t 1989 *T personal communication to FlyBase *u *F From A.J. Howells, to J. Merriam, February, 1989 *F Copy available from FlyBase by mail. # *U FBrf0075184 *a Scott *b M. *t 1992 *T personal communication to FlyBase *u *F From M. Scott, to J. Merriam, June 5, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075185 *a Bellen *b H.J. *t 1992 *T personal communication to FlyBase *u *F From H.J. Bellen, to J. Merriam, July 20th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075186 *a Rabinow *b L. *t 1993 *T personal communication to FlyBase *u *F From L. Rabinow, to J. Merriam, January 12th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075187 *a Kelley *b M.R. *t 1990 *T personal communication to FlyBase *u *F From M.R. Kelley, to J. Merriam, December 20th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075188 *a Hankins *b G. *c T. *d Homyk *e T.R.F. *f Wright *t 1990 *T personal communication to FlyBase *u *F From G. Hankins, T. Homyk and T. Wright, to J. Merriam, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075189 *a Shearn *b A. *t 1990 *T personal communication to FlyBase *u *F From A. Shearn, to J. Merriam, December 3rd, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075190 *a MacIntyre *b R. *t 1991 *T personal communication to FlyBase *u *F From R. MacIntyre, to J. Merriam, May 5th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075191 *a Marsh *b J. *t 198? *T personal communication to FlyBase *u *F From J. Marsh, to J. Merriam, 198? *F Copy available from FlyBase by mail. # *U FBrf0075192 *a Andrew *b D.J. *c B.S. *d Baker *t 1990 *T personal communication to FlyBase *u *F From D.J. Andrew and B. Baker, to J. Merriam, February 13th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075193 *a Busseau *b I. *t 1990 *T personal communication to FlyBase *u *F From I. Busseau, to J. Merriam, September 17th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075194 *a Walldorf *b U. *t 1992 *T personal communication to FlyBase *u *F From U. Walldorf, May 14th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075195 *a Beckingham *b K. *t 1992 *T personal communication to FlyBase *u *F From K. Beckingham, to J. Merriam, May 7th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075196 *a Phillips *b ?. *c A. *d Shearn *t 1993 *T personal communication to FlyBase *u *F From Phillips and A. Shearn, to J. Merriam, August 5th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075197 *a Jones *b R. *c W.M. *d Gelbart *t 1992 *T personal communication to FlyBase *u *F From R. Jones and W. Gelbart, to J. Merriam, May 5th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075198 *a Horodyski *b F. *t 1992 *T personal communication to FlyBase *u *F From F. Horodyski, to J. Merriam, May 5th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075199 *a Ellis *b H. *t 1990 *T personal communication to FlyBase *u *F From H. Ellis, to J. Merriam, October 19th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075200 *a Walldorf *b U. *c W.J. *d Gehring *t 1990 *T personal communication to FlyBase *u *F From U. Walldorf and W. Gehring, to J. Merriam, March 11th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075201 *a Xiong *b W.C. *c H. *d Okano *e J. *f Blendy *g C. *h Montell *t 1991 *T personal communication to FlyBase *u *F From W.C. Xiong, H. Okano, J. Blendy and C. Montell, to J. Merriam, June 19th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075202 *a Goralski *b T.J. *c K. *d Konrad *e A.P. *f Mahowald *t 1990 *T personal communication to FlyBase *u *F From Goralski, Konrad, and Mahowald, to J. Merriam, November 20th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075203 *a Ross *b J. *c M.B. *d Davis *e S. *f McKechnie *t 1992 *T personal communication to FlyBase *u *F From J. Ross, M.B. Davis, and S. McKechnie, to J. Merriam, May 26th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075204 *a Frank *b L. *c R. *d Cohen *t 1992 *T personal communication to FlyBase *u *F From L. Frank and R. Cohen, to J. Merriam, May 27th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075205 *a Bang *b A. *t 1992 *T personal communication to FlyBase *u *F From A. Bang, to J. Merriam, February 19th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075206 *a Levis *b R. *t 1990 *T personal communication to FlyBase *u *F From R. Levis, to J. Merriam, April 9th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075207 *a Beachy *b P. *t 1992 *T personal communication to FlyBase *u *F From P. Beachy, to J. Merriam, May 7th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075208 *a Wagner *b C. *t 1992 *T personal communication to FlyBase *u *F From C. Wagner, to J. Merriam, May 19th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075209 *a Zhong *b W. *t 1992 *T personal communication to FlyBase *u *F From W. Zhong, to J. Merriam, November 25th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075210 *a Binari *b R. *c N. *d Perrimon *t 1991 *T personal communication to FlyBase *u *F From R. Binari and N. Perrimon, to J. Merriam, June 18th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075211 *a Dearolf *b C.R. *t 1993 *T personal communication to FlyBase *u *F From C. Dearolf, to J. Merriam, August 15th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075212 *a Haynes *b S.R. *c G. *d Raychaudhuri *e A. *f Beyer *t 1991 *T personal communication to FlyBase *u *F From S. Haynes, G. Raychaudhuri and A. Beyer, to J. Merriam, March 18th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075213 *a Graziani *b F. *t 1990 *T personal communication to FlyBase *u *F From F. Graziani, to J. Merriam, December 20th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075214 *a Mansfield *b L. *c A. *d Shearn *t 1990 *T personal communication to FlyBase *u *F From L. Mansfield and A. Shearn, to J. Merriam, September 21st, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075215 *a Zuker *b C.S. *t 1992 *T personal communication to FlyBase *u *F From C. Zuker, to J. Merriam, May 7th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075216 *a Wright *b T.R.F. *t 1991 *T personal communication to FlyBase *u *F From T. Wright, to J. Merriam, December 12th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075217 *a Garrison *b K. *c J. *d Fessler *t 1990 *T personal communication to FlyBase *u *F From K. Garrison and J. Fessler, to J. Merriam, March 31st, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075218 *a Garrison *b K. *c J. *d Fessler *t 1990 *T personal communication to FlyBase *u *F From K. Garrison and J. Fessler, to J. Merriam, March 3rd, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075219 *a Hooper *b J. *t 1989 *T personal communication to FlyBase *u *F From J. Hooper, to J. Merriam, February 28th, 1989 *F Copy available from FlyBase by mail. # *U FBrf0075220 *a Fischer *b J. *t 1993 *T personal communication to FlyBase *u *F From J. Fischer, to J. Merriam, May 27th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075221 *a Wu *b C.T. *c J.M. *d Croop *t 1992 *T personal communication to FlyBase *u *F From C.T. Wu and J.M. Croop, to J. Merriam, May 5th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075222 *a Wehrli *b M. *c M.E. *d Wilcox *t 1990 *T personal communication to FlyBase *u *F From M. Wehrli and M. Wilcox, to J. Merriam, October 1990 *F Copy available from FlyBase by mail. # *U FBrf0075223 *a Schafer *b U. *t 1990 *T personal communication to FlyBase *u *F From U. Schafer, to J. Merriam, November 3rd, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075224 *a Schafer *b U. *t 1990 *T personal communication to FlyBase *u *F From U. Schafer, to J. Merriam, November 3rd, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075225 *a Wang *b C.I. *c R. *d Lehmann *t 1990 *T personal communication to FlyBase *u *F From C. Wang and R. Lehmann, to J. Merriam, December 20th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075226 *a Wieschaus *b E. *t 1992 *T personal communication to FlyBase *u *F From E. Wieschaus, to J. Merriam, June 22nd, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075227 *a Ephrussi *b A. *t 1990 *T personal communication to FlyBase *u *F From A. Ephrussi, to J. Merriam, December 20th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075228 *a Sass *b G. *t 1992 *T personal communication to FlyBase *u *F From G. Sass, to J. Merriam, June 22nd, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075229 *a Amero *b S.A. *t 1993 *T personal communication to FlyBase *u *F From S. Amero, to J. Merriam, May 6th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075230 *a Skoulakis *b E. *c R. *d Davis *t 1992 *T personal communication to FlyBase *u *F From E. Skoulakis and R. Davis, to J. Merriam, May 11th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075231 *a Lai *b Z.C. *t 1992 *T personal communication to FlyBase *u *F From Z-C. Lai, to J. Merriam, March 2nd, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075232 *a Barker *b D. *c J. *d Moore *e L. *f Dickinson *g R. *h Lehmann *t 1993 *T personal communication to FlyBase *u *F From D. Barker, J. Moore, L. Dickinson and R. Lehmann to J. Merriam, August 6th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075233 *a Nash *b D. *t 19?? *T personal communication to FlyBase *u *F From D. Nash, to J. Merriam. *F Copy available from FlyBase by mail. # *U FBrf0075234 *a Kelley *b R. *t 1992 *T personal communication to FlyBase *u *F From R. Kelley, to J. Merriam, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075235 *a Atkinson *b N. *t 1992 *T personal communication to FlyBase *u *F From N. Atkinson, to J. Merriam, February 2nd, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075236 *a O'Hare *b K. *t 1990 *T personal communication to FlyBase *u *F From K. O'Hare, to J. Merriam, November 23rd, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075237 *a Rutledge *b B.J. *t 1990 *T personal communication to FlyBase *u *F From B.J. Rutledge, to J. Merriam, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075238 *a Bellen *b H.J. *c A. *d Kolodkin *t 1993 *T personal communication to FlyBase *u *F From H.J. Bellen and A. Kolodkin, to J. Merriam, March 31st, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075239 *a O'Hare *b K. *t 1991 *T personal communication to FlyBase *u *F From K. O'Hare, to J. Merriam, September 25th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075240 *a Tricoire *b H. *t 1992 *T personal communication to FlyBase *u *F From H. Tricoire, to J. Merriam, May 27th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075241 *a Baumgartner *b S. *t 1993 *T personal communication to FlyBase *u *F From S. Baumgartner, to J. Merriam, February 19th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075242 *a Wampler *b S.L. *t 1992 *T personal communication to FlyBase *u *F From S.L. Wampler, to J. Merriam, May 5th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075243 *a Padgett *b R.W. *c W.M. *d Gelbart *t 1990 *T personal communication to FlyBase *u *F From R. Padgett and W. Gelbart, to J. Merriam, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075244 *a Crowley *b T. *c L. *d Jan *e Y.N. *f Jan *t 1993 *T personal communication to FlyBase *u *F From T. Crowley, L. Jan and Y-N. Jan, to J. Merriam, February 3rd, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075245 *a Kubli *b E. *t 1995 *T personal communication to FlyBase *u *F From E. Kubli, to J. Merriam, January 11th, 1995 *F Copy available from FlyBase by mail. # *U FBrf0075246 *a Pollock *b J. *t 1992 *T personal communication to FlyBase *u *F From J. Pollock, to J. Merriam, March 6th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075247 *a Brown *b L. *t 1992 *T personal communication to FlyBase *u *F From L. Brown, to J. Merriam, May 22nd, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075248 *a Boswell *b R. *t 1995 *T personal communication to FlyBase *u *F From R. Boswell, to J. Merriam, January 6th, 1995 *F Copy available from FlyBase by mail. # *U FBrf0075249 *a Kelley *b R. *t 1992 *T personal communication to FlyBase *u *F From R. Kelley, to J. Merriam, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075250 *a Samant *b S.A. *t 1990 *T personal communication to FlyBase *u *F From S.A. Samant, to J. Merriam, January 24th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075251 *a Deobagkar *b D. *t 1992 *T personal communication to FlyBase *u *F From D. Deobagkar, to J. Merriam, July 16th, 1992 *F Copy available from FlyBase by mail. # *U FBrf0075252 *a Jackson *b F.R. *t 1990 *T personal communication to FlyBase *u *F From F.R. Jackson, to J. Merriam, February 26th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075253 *a Stathakis *b D. *t 1991 *T personal communication to FlyBase *u *F From D. Stathakis, to J. Merriam, December 12th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075254 *a Atkinson *b N. *t 1993 *T personal communication to FlyBase *u *F From N. Atkinson, to J. Merriam, June 4th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075255 *a Yun *b B. *t 1993 *T personal communication to FlyBase *u *F From B. Yun, to J. Merriam, January 12th, 1993 *F Copy available from FlyBase by mail. # *U FBrf0075256 *a Voelker *b R.A. *t 1990 *T personal communication to FlyBase *u *F From R. Voelker, to J. Merriam, December 26th, 1990 *F Copy available from FlyBase by mail. # *U FBrf0075257 *a Burtis *b K. *t 1991 *T personal communication to FlyBase *u *F From K. Burtis, to J. Merriam, January 6th, 1991 *F Copy available from FlyBase by mail. # *U FBrf0075258 *a Carlson *b E.A. *t 1990 *T personal communication to FlyBase *u *F From Carlson to J. Merriam, 23 November 1990. *F Not available from FlyBase. # *U FBrf0075259 *a Davidson *b N. *t 1990 *T personal communication to FlyBase *u *F From N. Davidson et al. to J. Merriam, 1 October 1990. *F Not available from FlyBase. # *U FBrf0075260 *a Stephenson *b E. *t 1994 *T personal communication to FlyBase *u *F From ESTEPHEN@biology.as.ua.edu Fri Jul 1 15:09:41 1994 *F From: 'Ed' *F Organization: University of Alabama Dept. of Biol *F To: ma11 *F Date: Fri, 1 Jul 1994 11:39:57 CST6CDT *F Subject: Re: help for FlyBase *F X-Pmrqc: 1 *F Priority: normal *F X-Mailer: PMail v3.0 (R1a) *F Content-Length: 851 *F Status: RO *F X-Lines: 20 *F Michael: *F Dp(1;f)q2 was constructed by irradiating T(X;Y)B36, (from Johm *F Meerriam, broken at 5C and YS), and selecting for survival of the *F proximal portion of the X, that marked by y+. The resulting Dp covers *F sww and Df(1)JF5 at 5E. The exact breakpoints have not been mapped, *F but can be guessed at. Flies of the genotype rux / Dp q2 variegate *F for rux. Since rux is supposed to be within 5C, Dp q2 probably *F retains the original 5C breakpoint of B36. The proximal breakpoint is *F not so clear. Dp q2 covers vs, but not dx, shf or cm, placing the *F breakpoint very roughly between 5F and 6C. X / Dp(1;f)q2 males are *F sterile; X / Dp(X;f)q2 / Y are fertile. Dp(1;f)q2 is bb-. *F There is no published reference for this duplication. You have my *F permission to cite it as Ed Stephenson, personal communication. *F Is this what you needed? *F Ed Stephenson # *U FBrf0075261 *a Shearn *b A. *t 1994 *T personal communication to FlyBase *u *F From bio_cals@jhuvms.hcf.jhu.edu Mon Jul 11 18:33:30 1994 *F Date: Mon, 11 Jul 1994 13:24:37 -0400 (EDT) *F Date-Warning: Date header was inserted by JHUVMS.HCF.JHU.EDU *F From: bio_cals@jhuvms.hcf.jhu.edu (Allen Shearn) *F Subject: Re: FlyBase help *F To: ma11 *F Content-Transfer-Encoding: 7BIT *F Content-Length: 594 *F Status: RO *F X-Lines: 23 *F Dear Michael, *F Sorry for the delayed response. *F T(1;3)ash13.1 *F Induced by gamma rays; the third chromosome breakpoint is 76B6-11 *F Reference= Tripoulas et al., 1994 Genetics 137: 1-12 *F Sincerely, *F Allen *F ALLEN SHEARN *F Department of Biology Johns Hopkins University Baltimore, MD 21218 *F phone 410-516-7285 FAX 410-516-5213 # *U FBrf0075262 *a Moses *b K. *t 1994 *T personal communication to FlyBase *u *F From kmoses@mizar.usc.edu Thu Aug 4 18:29:29 1994 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 4 Aug 1994 11:23:56 -0800 *F To: ma11 *F From: kmoses@mizar.usc.edu (Kevin Moses) *F Subject: Re: help FlyBase & feel good *F Status: RO *F Content-Length: 3006 *F X-Lines: 86 *F Hi Mike, *F My appologies, here is a list of the chromosomes that I checked personally *F (I note the ones I still keep): *F OTHER CHROMOSOMES I CHECKED *F Df(3R)P14 90B3-8 to 91B1-2 (Ed Lewis chromosome-he has it end in 91A) *F Df(3R)cha-m7 90E-F to 91F5-13 *F MY GLASS MUTANT CHROMOSOMES *F Note: the mutagenized fly was a trans heterozygote between P(w+) A2-1 (a *F lethal insert at 91C) and P(w+)D3 (a viable, at 90E-F). Thus many of the *F chromosomes bear A2-1, and therefore that additional lethal locus. *F Df(3R)gl-BX1 gl- and lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F Df(3R)gl-BX2 gl- and lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation (I KEEP THIS STOCK) *F Df(3R)gl-BX3 90F to 91A3-8 (I KEEP THIS STOCK) *F Df(3R)gl-BX4 90F to 91A3-8 (probably the same as BX3, came from the *F same cross) (I KEEP THIS STOCK) *F Df(3R)gl-BX5 small, viable deletion (~4kb), within glass (I KEEP THIS STOCK) *F In(3R)gl-BX6 breaks are 84E and 91A, homozygous lethal, but viable over *F BX3, thus lethality is likely to be associated with the 84E breakpoint. (I *F KEEP THIS STOCK) *F Df(3R)gl-BX7 gl- and lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation (I KEEP THIS STOCK) *F Df(3R)gl-BX8 9011 to 91A6-8 also lethal with A2-1 (I KEEP THIS STOCK) *F Df(3R)gl-BX9 gl- and lethal with itself, viable with P(w+)A2-1, but no *F cytologically or Southern blot visible aberation (I KEEP THIS STOCK) *F Df(3R)gl-BX10 90C7-8 to 91B1 gl- and lethal with itself, and P(w+)A2-1 *F Df(3R)gl-BX11 gl- and lethal with itself, viable with P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F Df(3R)gl-BX12 90D3-6 to 91B5-8 gl- and lethal with itself, and *F P(w+)A2-1 *F Df(3R)gl-BX13 gl- and lethal with itself, viable with P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F Df(3R)gl-BX14 gl- and lethal with itself, viable with P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F OTHER (GLASS+) CHROMOSOMES FROM THE SAME SCREEN (DETECTED AS LOSS OF W+) *F l(3)BX1 gl+, lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F l(3)BX2 gl+, lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F l(3)BX3 gl+, lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F l(3)BX4 gl+, lethal with itself, and P(w+)A2-1, but no *F cytologically or Southern blot visible aberation *F l(3)BX5 91B1-2 to 91D1-2 gl+, lethal with itself, and P(w+)A2-1 *F l(3)BX6 90C9-10 to 90F2-11 gl+, lethal with itself,viable with *F P(w+)A2-1 *F In(3R)BX7 91B1-2 to 93F11-14 gl+, lethal with itself, and P(w+)A2-1 *F I left all these stocks with Gerry, I don't know which he still keeps. *F Also Don Gailey had his similar collection from this region. *F All the best, Kevin *F Kevin Moses at USC # *U FBrf0075263 *a Bairoch *b A. *t 1994 *T personal communication to FlyBase *u *F From BAIROCH%cmu.unige.ch%PPSW.CAM.AC.UK@phx.cam.ac.uk Sat Aug 6 02:21:05 1994 *F Date: Sat, 06 Aug 1994 03:12:55 +0000 (WET-DST) *F From: Amos Bairoch *F Subject: Drosophile ferredxin like orf *F To: ma11@phx.cam.ac.uk *F X-Envelope-To: ma11@phx.cam.ac.uk *F X-Vms-To: ASHBURNER *F X-Vms-Cc: BAIROCH *F Mime-Version: 1.0 *F Content-Transfer-Encoding: 7BIT *F Status: RO *F Content-Length: 1551 *F X-Lines: 43 *F Dear Michael, *F here is the adrenodoxin-like protein found by Ken Rudd which I *F briefly mentionned at Hinxton during the meeting. *F ID ADXH_DROME STANDARD; PRT; 95 AA. *F AC P37193; *F DT 01-OCT-1994 (REL. 30, CREATED) *F DT 01-OCT-1994 (REL. 30, LAST SEQUENCE UPDATE) *F DT 01-OCT-1994 (REL. 30, LAST ANNOTATION UPDATE) *F DE HYPOTHETICAL ADRENODOXIN-LIKE 10.5 KD PROTEIN IN HSP67BC 3'REGION. *F OS DROSOPHILA MELANOGASTER (FRUIT FLY). *F RN [1] *F RP SEQUENCE FROM N.A. *F RM 88118925 *F RA PAULI D., TONKA C.H.; *F RL J. MOL. BIOL. 198:235-240(1987). *F RN [2] *F RP IDENTIFICATION. *F RA RUDD K.E.; *F RL UNPUBLISHED OBSERVATIONS (JUL-1994). *F CC -!- SIMILARITY: BELONGS TO THE ADRENODOXIN/PUTIDAREDOXIN FAMILY. *F CC -!- CAUTION: IT IS PROBABLE THAT THIS PROTEIN STARTS BEFORE THE *F CC MET-1 SHOWN HERE AND THAT THE REAL INITIATOR MET IS HIDEN BY *F CC A FRAMESHIFT. *F DR EMBL; X06542; DMHSPG3. *F DR FLYBASE; ????; ????. *F KW HYPOTHETICAL PROTEIN; IRON-SULFUR; ELECTRON TRANSPORT. *F FT METAL 5 5 IRON-SULFUR (2FE-2S) (BY SIMILARITY). *F FT METAL 11 11 IRON-SULFUR (2FE-2S) (BY SIMILARITY). *F FT METAL 14 14 IRON-SULFUR (2FE-2S) (BY SIMILARITY). *F FT METAL 51 51 IRON-SULFUR (2FE-2S) (BY SIMILARITY). *F SQ SEQUENCE 95 AA; 10539 MW; 44728 CN; *F MEGACEASLA CTTCHVYVQH DYLQKLKEAE EQEDDLLDMA PFLRENSRLG CQILLDKSME *F GMELELPKAP GTSTSMGTSQ GHINIIVISR IINNI *F // *F Best regards *F Amos # *U FBrf0075264 *a Tartof *b K.D. *t 1993 *T personal communication to FlyBase *u *F From KD_TARTOF@super.rm.fccc.edu Thu Sep 2 14:51:02 1993 *F Date: Thu, 2 Sep 93 09:47:28 EST *F From: Kenneth D. Tartof *F Subject: DAKT1 *F To: m.ashburner@gen.cam.ac.uk *F X-Mailer: LeeMail 1.2.4 *F Content-Length: 1261 *F Status: RO *F X-Lines: 37 *F Dr. Kenneth D. Tartof *F Fox Chase Cancer Center *F 7701 Burholme Avenue *F Philadelphia, PA 19111 *F PHONE: (215) 728-2473 *F FAX: (215) 728-3574 *F E-MAIL: KD_TARTOF@FCCC.EDU *F Dear Michael, *F The sequence you refer to is the Drosophila 'homolog' related to the vertebrate *F proto-oncogene, Akt. I've named it Dakt1 and it maps to 3R at 89BC. Akt is a *F serine-threonine protein kinase whose carboxyterminal catalytic domain is *F closely related to the catalytic domains of all known members of the protein *F kinase C (PKC) family. However, Akt differs from PKC in its N-terminal portion *F which contains a region related to the SH2 domain of the cytoplasmic tyrosine *F kinases and other signaling proteins. Comparison between the coding regions of *F c-akt and Dakt1 revealed 64.6% identity at the nucleotide level and 76.5% *F similarity at the amino acid level. The highest degree of homology was *F detected in the SH2-like domain (68.3% similarity at the amino acid level) and *F the catalytic domain (83.3% similarity). *F The paper describing all of this is in press in Oncogene. *F Hope this is of some help. Let me know if you need more info. *F Best regards, *F Kenneth # *U FBrf0075265 *a Zhang *b N. *t 1993 *T personal communication to FlyBase *u *F From zhangn@rnisd0.dnet.roche.com Thu Sep 2 17:25:14 1993 *F From: zhangn@rnisd0.dnet.roche.com *F Date: Thu, 2 Sep 93 12:23:16 EDT *F To: m.ashburner@gen.cam.ac.uk *F Subject: sequence X69677 *F Content-Length: 270 *F Status: RO *F X-Lines: 4 *F Dear Michael, *F Thanks for the Fax. The sequence I submitted to EMBL (X69677) is homologous to the ribosomal protein 1024 of Dityostelium disoidium, the 40s ribosomal protein ys11 of S. cerevisiae and a 22 kd proteinfrom from Trypanosoma brucei. *F All the best. *F Nian Zhang # *U FBrf0075266 *a Hawley *b S.R. *t 1993 *T personal communication to FlyBase *u *F From srhawley@ucdavis.edu Tue Sep 7 03:03:23 1993 *F From: srhawley@ucdavis.edu *F Date: Mon, 6 Sep 93 18:55:33 PDT *F To: m.ashburner@gen.cam.ac.uk *F Subject: nod nomenclature *F Content-Length: 192 *F Status: RO *F X-Lines: 9 *F Dear Dr. Ashburner, *F I am responding for Scott Hawley; the Genbank submission is *F in error with respect of nod. nod was originally named *F mei-254 (that is the original allele). *F Kim S. McKim # *U FBrf0075267 *a Mahaffey *b J.W. *t 1993 *T personal communication to FlyBase *u *F From mahaffey@unity.ncsu.edu Thu Sep 30 15:23:57 1993 *F Posted-Date: Thu, 30 Sep 1993 10:21:57 -0400 (EDT) *F From: mahaffey@unity.ncsu.edu *F Subject: Re: flybase *F To: ma11@gen.cam.ac.uk (ma11) *F Date: Thu, 30 Sep 1993 10:21:57 -0400 (EDT) *F Cc: Jim_Mahaffey@ncsu.edu (Jim Mahaffey) *F X-Mailer: ELM [version 2.4 PL23b2/POP] *F Mime-Version: 1.0 *F Content-Type: text/plain; charset=US-ASCII *F Content-Transfer-Encoding: 7bit *F Content-Length: 1691 *F Status: RO *F X-Lines: 49 *F Michael, *F At present, there is no good name for the gene because I am not sure of *F its function or phenotype. In our DEVELOPMENT paper (118 pp203-214) we *F refer to the gene as 1.28, our stock number from our enhancer trap screen. *F We are searching hard for the function and phenotype. Deficiencies of *F the region do alter larval head development but I need to be certain *F that this is do to our gene. A hop-out screen is underway , and I hope *F to have more info soon. As for now, the gene is referred to as 1.28, *F lousy for a Drosophila gene but that's what we have. *F Jim Mahaffey *F > *F > Dear Jim *F > One of the functions of FlyBase is to keep track of all fly *F > nucleic acid databank accession numbers and assign these to the *F > 'correct' genes. *F > *F > I note your submission to Genbank no L07262 which says it *F > is a gene activated by Deformed. *F > *F > What gene does the sequence refer to ? *F > *F > Thanks for your help in keeping FlyBase uptodate & accurate ! *F > Michael *F > Michael Ashburner, Phone: +44 223 333969 *F > Department of Genetics, Fax: +44 223 333992 *F > Downing Streeet, e-mail: m.ashburner@gen.cam.ac.uk *F > Cambridge, CB2 3EH, England. *F > *F > *F -- *F Jim Mahaffey email Jim_Mahaffey@NCSU.EDU *F Dept. of Genetics voice 919 515-5791 *F North Carolina State University FAX 919 515-3355 *F Raleigh NC 27695 # *U FBrf0075268 *a Hey *b J. *t 1993 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Wed Oct 6 17:53:44 1993 *F From: Michael Ashburner (Genetics) *F Date: Wed, 6 Oct 93 17:56:54 BST *F To: hey@ocelot.rutgers.edu *F Subject: FlyBase Query *F Cc: ma11@gen.cam.ac.uk *F Content-Length: 4617 *F Status: RO *F X-Lines: 141 *F Dear Jordy *F Something tells me the attribution of species to 'Genbank' accessions in *F the following is wrong. Am I correct ? *F ie is SI simulans *F MA mauritiana *F SE sechellia *F all are in the sequence database as melanogaster ! *F I would like FlyBase to get it correct . *F Michael *F Michael Ashburner, Phone: +44 223 333969 *F Department of Genetics, Fax: +44 223 333992 *F Downing Streeet, e-mail: m.ashburner@gen.cam.ac.uk *F Cambridge, CB2 3EH, England. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13043 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain ME-NJ1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13044 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain ME-NJ2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13045 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain ME-LI1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13046 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain ME-LI2) DNA *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13047 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain ME-K1) DNA *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13048 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain ME-K2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13049 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SI-CA1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13050 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SI-CA2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13051 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SI-LI1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13052 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SI-LI2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13053 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SI-K1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13054 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain MA1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13055 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SI-K2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13056 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain MA2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13057 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain MA3) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13058 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain MA4) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13059 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain MA5) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13060 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain MA6) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13061 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SE-C1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13062 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SE-C2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13063 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SE-P1) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13064 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SE-P2) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13065 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SE-P3) DNA. *F DEFINITION Drosophila melanogaster zeste (z) gene, partial cds. *F ACCESSION L13066 *F KEYWORDS zeste protein. *F SOURCE Drosophila melanogaster (strain SE-P4) DNA. *F From HEY@ocelot.rutgers.edu Wed Oct 6 18:52:55 1993 *F Date: Wed, 6 Oct 1993 13:48 EDT *F From: HEY@ocelot.Rutgers.EDU *F Subject: Re: FlyBase Query *F To: ma11@gen.cam.ac.uk *F X-Envelope-To: ma11@gen.cam.ac.uk *F X-Vms-To: IN%'ma11@gen.cam.ac.uk' *F Content-Length: 83 *F Status: RO *F X-Lines: 2 *F you are correct, I messed up on the submission and have yet to correct things *F Jody # *U FBrf0075269 *a Brower *b D. *t 1993 *T personal communication to FlyBase *u *F From dan_brower@tikal.biosci.arizona.edu Sun Nov 21 15:35:46 1993 *F Return-Receipt-To: Dan Brower *F Date: Sun, 21 Nov 1993 08:29:30 +0000 (U) *F From: Dan Brower *F Subject: new gene name *F To: 'M. Ashburner' *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 1471 *F Status: RO *F X-Lines: 25 *F Subject: Time:8:22 AM *F OFFICE MEMO new gene name Date:11/21/93 *F Michael, *F I understand that you are the current curator of fly genetics, so I am *F writing to try to lay claim to a gene name. We have isolated a number of EMS *F alleles of the gene that encodes the alphaPS1 integrin subunit. These were *F generated in a FRT/FLP screen looking for mutations that caused wing *F blisters, and we would like to name the gene something like 'multiple *F edematous wings'. Although this seems like a rather obtuse name, you will *F notice that it was chosen for the abbreviation, mew, which happen to be the *F initials of our recently deceased friend, Michael Wilcox. I have spoken with *F most of the people in the field about this name (including Nick in your *F institution), and there is general agreement that it would be a fitting *F gesture. I realize that genes already identified molecularly are supposed to *F be named after the protein, but we think an exception is justified here, and *F besides, the mutations were originally generated based on a phenotypic screen *F for blister mutants, and we have only very recently confirmed that they are *F probably in the alphaPS1 gene. I am writing to you to make certain there is *F no other mew gene (I don't find one in the database), and to ask to reserve *F the name, since it will be some time before any of this is published. Is *F this possible? *F Thanks, *F Danny Brower # *U FBrf0075270 *a Lepesant *b J.A. *t 1993 *T personal communication to FlyBase *u *F From LEPESANT@arthur.citi2.fr Fri Dec 24 08:12:03 1993 *F Date: Fri, 24 Dec 1993 9:08:21 +0100 (WET) *F From: LEPESANT@arthur.citi2.fr *F Subject: RE: sry *F To: ma11@gen.cam.ac.uk *F X-Vmsmail-To: SMTP%'ma11@genetics.cambridge.ac.uk' *F Content-Length: 873 *F Status: RO *F X-Lines: 16 *F Michael, *F only serendipity alpha is a cellularisation gene. *F sry beta and delta encode Zn finger proteins. Alain Vincent in *F Toulouse is working on them. *F We had isolated (using sry beta and delta probes ) *F a gene we called serendipity homologue 1. It encodes also a Zn finger *F protein. We have renamed it pourquoi pas?. This is the one which may *F correspond to the wings down gene of Morgan. However wings down *F alleles were lost. *F This the sry story. Alain Vincent wants to keep the sry alpha, beta and delta *F names. I find it a bit confusing but it is probably too late by now. *F We are very interested by your deficiencies in the 70 region *F and sopecially the one which may breajk into the Fbp1 region. We are *F very seriously considering isolating mutants of thet gene. *F As you said we ought to know about it function. *F With best wishes et bonne annee. Jean-Antoine # *U FBrf0075271 *a Hirsh *b J. *t 1994 *T personal communication to FlyBase *u *F From jh6u@virginia.edu Mon Sep 5 12:31:26 1994 *F Date: Sat, 3 Sep 94 16:10:49 EDT *F From: Jay Hirsh *F Subject: flybase.correction *F To: flybase@morgan.harvard.edu *F X-Mailer: VersaTerm Link v1.1.5 *F Content-Length: 1907 *F flybase@morgan.harvard.edu *F Please note the following correction to the gene entry for Ddc (DOPA *F decarboxylase), with the corrected lines marked **: *F jay hirsh *F Old form: *F Molecular biology data : *F Ddc was cloned by a two-step screen, first screening *F for genes encoding hypodermally expressed mRNA's and *F then screening these clones by in situ hybridization *F (Hirsh and Davidson, 1981). *F The sequence of Ddc and flanking DNA has been *F determined by the Hirsh and Marsh laboratories: Morgan, Johnson and *F Hirsh, *F 1986; Eveleth, Geitz, Spencer, Nargan, Hodgetts and *F Marsh, 1986). Whereas these *F sequences are in general agreement, there are several *F differences, including several that affect the *F predicted reading frames. Ddc *F encompasses four exons, labelled A, B, C and D, of *F which B, C and D are protein encoding. The major mRNA *F encoded by Ddc is in the hypoderm, a 2.1-kb mRNA *F containing all four exons... *F Corrected: *F Molecular biology data : *F Ddc was cloned by a two-step screen, first screening *F for genes encoding hypodermally expressed mRNA's and *F then screening these clones by in situ hybridization *F (Hirsh and Davidson, 1981). *F The sequence of Ddc and flanking DNA has been *F determined by the Hirsh and Marsh laboratories: Morgan, Johnson and *F Hirsh, *F 1986; Eveleth, Geitz, Spencer, Nargan, Hodgetts and *F Marsh, 1986). Whereas these *F sequences are in general agreement, there are several *F differences, including several that affect the *F predicted reading frames. Ddc *F encompasses four exons, labelled A, B, C and D, of *F which B, C and D are protein encoding. The major mRNA *F encoded by Ddc is in the hypoderm, a 2.1-kb mRNA *F **containing the three exons A,C, and D, whereas the CNS isoform, *F **generated by alternative splicing, contains all four exons *F **(Morgan, Johnson and Hirsh, 1986).... # *U FBrf0075272 *a Lorenz *b L. *t 1994 *T personal communication to FlyBase *u *F From LORENZ@rascal.med.harvard.edu Thu Sep 29 22:32:05 1994 *F Date: Thu, 29 Sep 1994 17:23:12 -0400 (EDT) *F From: LORENZ@rascal.med.harvard.edu *F To: FLYBASE-UPDATES@morgan.harvard.edu *F Subject: midline *F Content-Length: 833 *F I and others have noticed information in FlyBase and DIS 73 that I *F believe is incorrect. It would be helpful to others if these corrections *F were noted: *F 1) midline most likely does not encode nuclear lamin. midline *F is not removed genetically by Df(2)clh4, but nuclear lamin is (by *F molecular inspection). *F 2) midline mutants complement gdh1 genetically. *F 3) midIIS21 has been remapped relative to thv and cl to 25D5-E1 *F (this was in the Lorenz and Perrimon fly meeting abstract of this year). *F 4) midIK97 has not been remapped. We originally thought midline *F was in 25F1-2 because of Szidonya and Reuter's placement (1988), before *F I remapped it relative to tkv and cl. *F If you have questions, you can reach me by Email (Lorenz@Rascal.Med.Harvard. *F Edu) or call me at 432-7548. Lori Lorenz (Perrimon lab, Harvard Medical *F School). # *U FBrf0075273 *a Wolin *b S.L. *t 1994 *T personal communication to FlyBase *u *F From Sandra_Wolin%quickmail.cis.yale.edu@yaleads.ycc.yale.edu Sun Oct 9 19:53:02 1994 *F Date: 9 Oct 1994 14:51:02 -0400 *F From: 'Sandra Wolin' *F Subject: Re: Help FlyBase Please *F To: 'ma11' *F X-Mailer: Mail*Link SMTP-QM 3.0.1 GM *F Content-Length: 2509 *F REPLY o REPLY o REPLY o REPLY *F RE>Help FlyBase Please *F Dear Dr. Ashburner, *F It is fine with us if you refer to the gene as La. We stand by our mapping *F of the gene to 38C. Although my laboratory had no prior experience with *F mapping genes to polytene chromosomes, the squashes were independently *F examined by both Lynn Cooley and Carl Hashimoto, who agreed with our *F identification. I would also like to point out that we strongly disagree *F with the idea (in the Tolias paper) that Drosophila La is a DNA-binding *F protein. First, the potential leucine zipper he noted is not *F phylogenetically conserved. Second, virtually all members of this class of *F RNA-binding proteins will non-specifically bind to DNA in vitro. Lastly, we *F examined the RNAs bound by the Drosophila La protein in vivo (see Yoo and *F Wolin) and found that they were identical to the RNAs bound by the mammalian *F La protein. *F Date: 10/8/94 6:13 AM *F To: Sandra Wolin *F From: ma11 *F Dear Peter and Dr Wolin *F I have just realised that the gene cloned by you guys *F (MCB 14:5123 and MCB 14:5412) is the same ! However you differ *F in the in situ determined map position - Peter says 36A and *F Dr. Wolin says 38C. The photos in Yoo & Wolin look consistent *F with 38C, tho' I have not studied them carefully. Is there *F are resolution of this difference ? Also do you both agree that *F we call the gene by the symbol La ? *F Thanks for helping FlyBase. *F Peter - best regards; I stayed with Fotis earlier this week. *F Michael *F Michael Ashburner, Phone: +44 223 333969 *F Department of Genetics, Fax: +44 223 333992 *F Downing Streeet, e-mail: *F m.ashburner@gen.cam.ac.uk *F Cambridge, CB2 3EH, England. *F From tolias@phri.nyu.edu Mon Oct 10 15:28:25 1994 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 10 Oct 1994 10:33:05 -0400 *F To: ma11 *F From: tolias@phri.nyu.edu (Peter Tolias) *F Subject: Re: Help FlyBase Please *F Content-Length: 459 *F Dear Michael, *F We have rexamined our previous and repeated our D-La in situs and we have *F also probed P1 southern blots. I now agree that our initial mapping at 38A *F was misplaced. Our revised and detailed mapping places Drosophila La at *F 38C2. *F La is not a symbol but the actual spelling of the human homolog. Hence, *F the fly gene should be refered to as Drosophila La or as we refer to it in *F our paper as D-La. *F Sorry about the mix up. Many regards *F Peter *F From Sandra_Wolin%quickmail.cis.yale.edu@yaleads.ycc.yale.edu Tue Oct 11 15:06:10 1994 *F Date: 11 Oct 1994 10:05:20 -0400 *F From: 'Sandra Wolin' *F Subject: Re: Help FlyBase Please *F To: 'ma11' *F X-Mailer: Mail*Link SMTP-QM 3.0.1 GM *F Content-Length: 1999 *F REPLY o REPLY o REPLY o REPLY *F RE>>Help FlyBase Please *F Thank you for the confirmation of our mapping data. I am a little mystified *F by Peter Tolias' objection to calling the gene La. Although I am not a *F Drosophilogist, the cDNA that we both identified clearly encodes the La *F protein in Drosophila. From my reading of the redbook, it appears that no *F fly genes are designated D- for Drosophila. If Drosophila actin is act and *F Drosophila alcohol dehydrogenase is adh, then the Drosophila La protein *F should be La. *F If it helps at all I can tell you the derivation of the name La. The protein *F is recognized by anti-La antibodies from patients with certain autoimmune *F diseases. 'La' stands for the first two letters of the patient's name in *F which these autoantibodies were first described. So the patient's name could *F have been Lane, Lawson, Laverty, etc. *F Anyway, I hope this helps or at least amuses you. Thanks again for the *F message. *F Sandra Wolin # *U FBrf0075274 *a Ising *b G. *t 19?? *T personal communication to FlyBase *u *F Localized TE positions on polytene chromosomes *F from G. Ising to M. Ashburner. *F Copy available from FlyBase by mail # *U FBrf0075275 *a Kreber *b R. *t 1994 *T personal communication to FlyBase *u *F These data are from a fax from Bob Kreber to John Roote, March 17 1994. *F Copy available from FlyBase by mail. # *U FBrf0075276 *a Jackson *b R. *t 1994 *T personal communication to FlyBase *u *F From JACKSON@sci.wfeb.edu Thu Jun 30 16:12:28 1994 *F Date: Thu, 30 Jun 1994 13:43:35 -0400 (EDT) *F From: JACKSON@sci.wfeb.edu *F Subject: m alleles *F To: m.ashburner@gen.cam.ac.uk *F X-Vms-To: IN%'m.ashburner@gen.cam.ac.uk' *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 678 *F Status: RO *F X-Lines: 12 *F Michael, m[mw] is a gamma ray-induced m allele described in Newby et al., *F Genetics 128: 571-582 (1991). It was isolated by Linda Restifo in a screen for *F unrelated mutations and provided to me. This mutation is associated by two *F distinct molecular lesions within an approximately 1-kb segment at molecular *F position +28 in our unpublished chromosome walk through the 10E1-2 interval. *F m[38] is probably In(1)m[38c] which is in the red book. We have found at least *F two molecular lesions in this strain within a 10-kb segment of our walk *F (position +25 to +35), but don't know if either causes the m phenotype of this *F inversion mutant. I hope this information is helpful. *F Rob # *U FBrf0075277 *a Bairoch *b A. *t 19?? *T personal communication to FlyBase *u *F From: Amos Bairoch *F To: ma11@phx.cam.ac.uk *F Subject: Two things *F X-Envelope-To: ma11@phx.cam.ac.uk *F X-Vms-To: ASHBURNER *F X-Vms-Cc: BAIROCH *F Mime-Version: 1.0 *F Content-Transfer-Encoding: 7BIT *F Content-Length: 2732 *F Dear Mike, *F 2) The gene coding for this entry is 'bundled' with trp, yet it *F encode what look like a real protein highly similar to an E.coli sequence. *F It therefore should get its own gene name and Flybase number: *F ID TRLC_DROME STANDARD; PRT; 264 AA. *F AC P36951; *F DT 01-JUN-1994 (REL. 29, CREATED) *F DT 01-JUN-1994 (REL. 29, LAST SEQUENCE UPDATE) *F DT 01-JUN-1994 (REL. 29, LAST ANNOTATION UPDATE) *F DE TRANSIENT RECEPTOR POTENTIAL LOCUS C PROTEIN PRECURSOR. *F OS DROSOPHILA MELANOGASTER (FRUIT FLY). *F OC EUKARYOTA; METAZOA; ARTHROPODA; INSECTA; DIPTERA. *F RN [1] *F RP SEQUENCE FROM N.A. *F RA WONG F., YUH Z., SCHAEFER E.L., ROOP B.C., ALLY A.H.; *F RL SOMAT. CELL MOL. GENET. 13:661-669(1987). *F CC -!- SIMILARITY: STRONG, TO E.COLI GIP. *F DR EMBL; M18635; DMTRPB. *F DR PIR; S29144; S29144. *F DR FLYBASE; 03861; TRP. *F KW SIGNAL. *F FT SIGNAL 1 14 POTENTIAL. *F FT CHAIN 15 264 TRANSIENT RECEPTOR POTENTIAL LOCUS C *F FT PROTEIN. *F SQ SEQUENCE 264 AA; 29093 MW; 372556 CN; *F MALKFAANLN FLFTERATSI AERIRLAHQN GFRAVEIPYP EGETSDVVSA VKETGVVVSL *F VNLAFDKSDD QLRFGSTSVP GSEKLFRSQL DATIDFARQV NCGKIHLTAG LFKGGQESDY *F TKTYTANLKI AADSLRASKM IGVIEPINKY AVPGYYMNSY SKAAGILADV AADNIQLLAD *F LYHLQHLHGN VSKTLEEYKA LIGHFQIAQV PHRHEPDVSG ELDYGFVFKA LQEFGYDGWI *F GCEYKPKTTT VEGLGWVSKL GYTL *F // *F TRLC_DROME MALKFAANLNFLFTERATSIAERIRLAHQNGFRAVEIPYP-EGETSDVVS *F GIP_ECOLI M-LRFSANLSMLFGE--YDFLARFEKAAQCGFRGVEFMFPYDYDIEELKH *F * *.*.***..**.* .. .*. * * ***.**. .* . ..... *F *F TRLC_DROME AVKETGVVVSLVNLAFDKSDDQLRFGSTSVPGSEKLFRSQLDATIDFARQ *F GIP_ECOLI VLASNKLEHTLHNLPAGDWAAGER-GIACIPGREEEFRDGVAAAIRYARA *F .. .. . .* **. .. .. * * ...**.*. **. ..*.* .**. *F *F TRLC_DROME VNCGKIH-LTAGLFKGGQESDYTKTYTANLKIAADSLRASKMIGVIEPIN *F GIP_ECOLI LGNKKINCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPIN *F .. **. *.. * ... * ..**. **. *. .... .***** *F *F TRLC_DROME KYAVPGYYMNSYSKAAGILADVAADNIQLLADLYHLQHLHGNVSKTLEEY *F GIP_ECOLI HFDIPGFHLTGTRQALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQW *F ....**..... ..* ...**. *... *.**.*...*....*.... *F *F TRLC_DROME KALIGHFQIAQVPHRHEPDVSGELDYGFVFKALQEFGYDGWIGCEYKPKT *F GIP_ECOLI ADKIGHLQIADNPHRGEPG-TGEINYDYLFKVIENSDYNGWVGCEYKPQT *F . ***.***. *** **. .**..*...**.... .*.**.******.* *F *F TRLC_DROME TTVEGLGWVSKLGYTL *F GIP_ECOLI TTEAGLRWMDP--YR- *F ** .** *.. * *F *F Best regards *F Amos # *U FBrf0075278 *a Haynes *b S.R. *t 1994 *T personal communication to FlyBase *u *F From shaynes%lmgvax.dnet@dxi.nih.gov Mon May 2 19:49:07 1994 *F Date: Mon, 2 May 94 14:43:25 -0400 *F From: shaynes%lmgvax.dnet@dxi.nih.gov *F To: dxi::'flybase@morgan.harvard.edu'@dxi.nih.gov *F Subject: Flybase corrections *F Content-Length: 2246 *F Status: RO *F X-Lines: 47 *F Susan Haynes *F NIH *F Bldg.6B, Rm.3B331 *F Bethesda, MD 20892 *F phone: 301-496-7879 *F FAX: 301-496-0243 *F email: shaynes%lmgvax.dnet@dxi.nih.gov *F The following are corrections and additions to FlyBase entries. *F FlyBase id #: FBgn0001215 (Hrb98DE) *F Reference FBrf0059186 does NOT refer to this gene; this entire section should *F be deleted and the reference deleted from the Gene references section. *F Reference FBrf0052555 is the right paper. Items to be corrected/added to this *F section: genomic length of clone - 10; cDNA clone length - 1.4, 1.5, 2.3; open *F reading frame size(s) - 360, 361, 364, 365 aa. *F Reference FBrf0047212: GGx-repeat should not be listed as a function; it is a *F characteristic of the sequence. Saying it is opa-like is confusing; it is opa- *F like in that it is a 3 nt repeat, but the sequence is not related to opa. *F The name for the final reference is Raychaudhuri. *F FlyBase id #: FBgn0004903 (Hrb97D) *F I have problems with the Hrb97D name. It was originally named Rb97D, for RNA- *F binding protein at 97D; we do not know whether it is a heterogeneous nuclear *F RNA binding protein, so the Hrb designation conveys a meaning that may be *F incorrect. We have never referred to it as Hrb97D, so I would like to have the *F original name used. *F RNA in situ distribution: This refers to beta-galactosidase staining of testes *F in a line with an enhancer-trap element inserted in Rb97D. It is NOT an in situ. *F RNA space/time distribution: probably present in all stages, highest levels *F early larvae and pupae. *F Allele: Another allele, Rb97D[2], has been isolated; it is a deletion of the *F coding region generated by imprecise excision of the P element in Rb97D[1]. *F FlyBase id #: FBgn0004237 (Hrb87Fa) *F Hrb87Fa made sense when sqd was listed as Hrb87Fb; since sqd is listed as sqd, *F the 'a' should be dropped. *F Cytological map position: it should be Df(3R)red3l (not rod). *F Phenotypic information: Hrb1 should be referred to as Hrb98DE, which is the *F original name of the gene. Dan Lindsley renamed it Hrb1 for the Redbook, but *F later agreed with me that the original name was preferable. *F Gene References: As far as I know, FBrf0064394 does not refer to this gene. *F Please contact me if you have any questions regarding these corrections. *F Thanks very much. *F Sue Haynes # *U FBrf0075279 *a Jacobs-Lorena *b M. *t 1994 *T personal communication to FlyBase *u *F Fax from M. Jacobs-Lorena to Dan Lindsley 2 March 1994. Copy available *F by mail from FlyBase. # *U FBrf0075280 *a Matthews *b K. *t 1994 *T personal communication to FlyBase *u *F Personal communication from: Kathy Matthews, Indiana University *F To: FlyBase *F Subject: Df(3R)e-BS2 *F Date: 31 May 94 *F *F Redbook correction: p. 867, left column, Df(3R)e entry: 'Df(3R)e-B52' should read 'Df(3R)e-BS2' *F FlyBase Aberrations corrections: 'Df(3R)e-B52' should read 'Df(3R)e-BS2' *F *F Eric Baehrecke has reported to the Bloomington Stock Center that, based on in situs to this deficiency with probes from his walk through this region, *F the distal breakpoint of Df(3R)e-BS2 is 93F14-94A1. # *U FBrf0075281 *a Wright *b T.R.F. *t 1994 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Ted Wright, University of Virginia *F To: Bloomington Drosophila Stock Center *F Subject: DTS91 *F Dated: Oct 24, 1994 *F *F Background: A Bloomington stock received from Ted Wright contains a mutation he called DTS-91, now DTS91. No published reference was found *F so Ted was queried about the identify of this DTS. *F *F Information communicated: *F DTS91 was received from Dave Suzuki 1/18/69. Suzuki stated that DTS91 is located in the dp-b region close to clot - 'very *F tight' - and DTS91/DTS91 dies at room temp. Whether the recessive lethality is caused by DTS91 or by other lethals on the chromosome is not known. *F Its location suggests that DTS91 is an allele of the group of dominant cold sensitive mutants that Suzuki's lab isolated around the same time *F (see Rosenbluth et al. 1972 Genetics 70:75-86; see also Suzuki and Procunier 1969 PNAS 62:369-376), although Suzuki was not able to *F provide the new designation (used in the publication) for DTS91. The conclusion is that DTS91 is one of the 11 DTSs that mapped between *F dp and b reported in the Suzuki-Procunier paper. None of the DTSs listed in the Red Book map between dp and b, so DTS91 may be the *F only survivor from this region of Suzuki and Procunier's work. # *U FBrf0075282 *a Bairoch *b A. *t 1994 *T personal communication to FlyBase *u *F From BAIROCH%cmu.unige.ch%PPSW.CAM.AC.UK@phx.cam.ac.uk Mon Oct 31 15:56:06 1994 *F Date: Mon, 31 Oct 1994 16:53:14 +0000 (WET-DST) *F From: Amos Bairoch *F Subject: Error report *F To: ma11@phx.cam.ac.uk *F X-Envelope-To: ma11@phx.cam.ac.uk *F X-Vms-To: ASHBURNER *F X-Vms-Cc: BAIROCH *F Mime-Version: 1.0 *F Content-Transfer-Encoding: 7BIT *F Content-Length: 1464 *F Dear Michael, *F what you call: *F 03517; STA. *F 10239; LAM-R. *F are the same, and is in fact a ribosomal protein: *F ID RSP4_DROME STANDARD; PRT; 270 AA. *F AC P38979; *F DT 01-FEB-1995 (REL. 31, CREATED) *F DT 01-FEB-1995 (REL. 31, LAST SEQUENCE UPDATE) *F DT 01-FEB-1995 (REL. 31, LAST ANNOTATION UPDATE) *F DE 40S RIBOSOMAL PROTEIN P40 (STUBARISTA PROTEIN) (LAMININ RECEPTOR *F DE HOMOLOG) (K14). *F GN STA. *F OS DROSOPHILA MELANOGASTER (FRUIT FLY). *F RN [1] *F RP SEQUENCE FROM N.A. *F RA MELNICK M.B., NOLL E., PERRIMON N.; *F RL GENETICS 135:553-564(1993). *F RN [2] *F RP SEQUENCE OF 18-270 FROM N.A. *F RC STRAIN=CANTON-S; *F RA KIM Y.J., BAKER B.S.; *F RL SUBMITTED (XXX-1991) TO EMBL/GENBANK/DDBJ DATA BANKS. *F CC -!- SUBCELLULAR LOCATION: CYTOPLASMIC. *F CC -!- CAUTION: WAS ORIGINALLY THOUGHT TO BE A LAMININ RECEPTOR. *F CC -!- SIMILARITY: BELONGS TO THE S2P FAMILY OF RIBOSOMAL PROTEINS. *F DR EMBL; M90422; DMP40A. *F DR EMBL; M77133; DMLAMR. *F DR FLYBASE; 03517; STA. *F DR FLYBASE; 10239; LAM-R. *F KW RIBOSOMAL PROTEIN. *F SQ SEQUENCE 270 AA; 30228 MW; 378458 CN; *F MSGGLDILSL KEDDITKMLV ATTHLGSENV NFQMEQYVYK RRADGVNILN LGKTWEKLQL *F AARAIVAIDN PSDIFVISSR PIGQRAVLKF AKYTDTTPIA GRFTPGAFTN QIQPAFREPR *F LLVVTDPNTD HQPIMEASYV NIPVIAFTNT DSPLRYIDIA IPCNNKSAHS IGLMWWLLAR *F EVLRLRGTIS RSVEWPVVVD LFFYRDPEEA EKEEAAAKEL LPPPKIEEAV DHPVEETTNW *F ADEVAAETVG GVEDWNEDTV KTSWGSDGQF *F // *F Best regards *F Amos # *U FBrf0075283 *a Fyrberg *b E. *t 1994 *T personal communication to FlyBase *u *F From BIO_AEF@jhuvms.hcf.jhu.edu Mon Oct 31 18:26:48 1994 *F Date: Mon, 31 Oct 1994 13:18:20 -0400 (EDT) *F From: BIO_AEF@jhuvms.hcf.jhu.edu *F Subject: actin-related genes *F To: m.ashburner@gen.cam.ac.uk *F X-Vms-To: IN%'m.ashburner@gen.cam.ac.uk' *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 191 *F Actr it is then. Thanks for your attention to the matter. Frankel's gene is *F indeed different than any of our four, and appears to encode a more *F distant relative. All the best, Eric Fyrberg # *U FBrf0075284 *a Simcox *b M. *t 1994 *T personal communication to FlyBase *u *F Date: Tue, 1 Nov 1994 16:50:43 -0500 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: ma11 *F From: asimcox@magnus.acs.ohio-state.edu (Amanda Simcox) *F Subject: Re: help *F Content-Length: 303 *F Dear Michael, *F I'm cringing as I look at the date on your Email. This is all I *F know about the two translocations. Hopefully, it will appear in D. Bio. as *F I am revising a manuscript for them. *F ddd gamma 4 T(2;3) 2R;64F *F ddd gamma 6 T(1;3) 15F-16A;64F *F Best wishes, Mandy Simcox *F Mandy Simcox # *U FBrf0075285 *a Brock *b H. *t 1994 *T personal communication to FlyBase *u *F From Hugh.Brock@mtsg.ubc.ca Tue Oct 25 16:25:38 1994 *F Date: Tue, 25 Oct 94 09:25:26 PDT *F From: Hugh.Brock@mtsg.ubc.ca *F To: eleanor@gen.cam.ac.uk *F X-Mts-Userid: HWB. *F Subject: ph410 breakpoint *F Content-Length: 375 *F Dear Eleanor: *F I am afraid that I have not localized the proximal breakpoint of ph410. *F What I remember from many years ago is that is is a big inversion with *F its proximal breakpoint in interval 13-14. Presumably this is the origin *F of the error that the breakpoint is in D13-14. I will do a squash and *F see if I can determine the breakpoint accurately. Cheers, Hugh Brock # *U FBrf0075286 *a Sparrow *b J.C. *t 1994 *T personal communication to FlyBase *u *F From jcs1@tower.york.ac.uk Fri Nov 4 14:37:41 1994 *F Date: Fri, 4 Nov 1994 11:32:25 +0000 *F From: JC Sparrow *F X-Sender: jcs1@tower.york.ac.uk *F To: michael ashburner *F Subject: Ifm(3)5 *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1197 *F Michael, It was good to speak with you yesterday. *F My first response to your request for information on Ifm(3)5 was wrong. *F It is not in Act88F. The correct answer probably explains why there is a *F gap in the database. *F Ifm(3)5 is a tropomyosin gene mutation in the Tm1 gene (symbol as in the *F current Flybase: there has until recently been confusion on the numbering *F of these genes, as you are probably aware, due to Eric Fyrberg using an *F opposite numbering system to everyone else who were using Bob Storti's.). *F The Ifm(3)5 allele is identical to Ifm(3)3 in having an identical insertion *F of an 8.8 kb copia-like mobile element inserted into the gene. *F Reference: Karlik C.C. and Fyrberg E.A. (1985). An insertion within a *F variably spliced Drosophila tropomyosin gene blocks accumulation of only *F one encoded isoform. Cell 41: 57-66 (FBrf0042041) *F I am sure this is the reason that Ifm(3)5 has dropped from sight. I do wonder *F about the Mogami and Hotta screen that produced these mutants as 3 of the *F Mhc alleles from it also turned out to be the same when analysed by Sandy *F Bernstein and others. *F Hope that is what you wanted. See you sometime (Atlanta if not before ?). *F All the best, John # *U FBrf0075287 *a Santamaria *b P. *t 1994 *T personal communication to FlyBase *u *F P. Santamaria fax to FlyBase, October 1994 *F Copy available from FlyBase by mail # *U FBrf0075288 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Fri Nov 4 13:07:12 1994 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 4 Nov 1994 13:07:01 +0000 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: minor problem with an ace table *F Content-Length: 880 *F Michael, *F Re: alleles used in crosses to PZs and PWs (have I got it right this time?): *F chif[WO18] - possibly = chif[1] which was called chif[WD]?P15 *F 35Bc[AR1] = 35Bc[1] *F 35Bc[HG1] = 35Bc[4] *F 35Bc[SF17] = 35Bc[6] *F 35Bd[SF6] = 35Bd[4] *F 35Cd[B14] = 35Cd[4] *F 35Cd[P34] - not in flybase as 35Cd allele, but as 35Dg[3] - it is a double. *F NB 35Cd[B8] = 35Cd[3] also used, but it complemented PZ17, so stock may be *F buggered. *F 35Dd[TE116] = 35Dd[TE35D] = 35Dd[4] for the Kiss alleles (8/7, 50/7, 91/9) *F 35Dd[GE1] = 35Dd[1] and 35Dd[P41] = 35Dd[3] for Sprad 5206, 5277 and 10427 *F 35Dd[P41] = 35Dd[3] for Sprad 5278 *F 35Df[P15] = 35Df[1] (semi-lethal with 144/23) *F 35Fc[AR144] - don't know new name, not in flybase. *F 35Fe[AS63] - ditto *F J *F Department of Genetics *F University of Cambridge *F Downing Street *F Cambridge *F CB2 3EH *F UK *F Tel: 44 223 333982 *F Fax: 44 223 333992 *F email: j.roote@gen.cam.ac.uk # *U FBrf0075289 *a Gould *b A. *t 1994 *T personal communication to FlyBase *u *F From: Rachel Drysdale (Genetics) *F Date: Mon, 7 Nov 94 15:46:54 GMT *F To: ma11@gen.cam.ac.uk *F Subject: Re: transposon names *F *F Michael *F Re: P[PS2(integrin)-promoter/lacZ construct of R. White] *F I just talked to Alex Gould, who began the work on eg[T6], about this. *F This construct has the inflated 5'region fused as a translational *F fusion (small (Alex didn't know exactly how small) number of if amino *F acids), and marked with ry[+]. I guess that makes it P{ry[+], if:lacZ} *F It was originally built by Maria Leptin. This is all the info he had. *F Rach # *U FBrf0075290 *a Hartl *b D. *t 1994 *T personal communication to FlyBase *u *F From d_hartl@nocmsmgw.harvard.edu Thu Nov 10 12:49:21 1994 *F Date: 10 Nov 1994 07:47:57 -0400 *F From: 'd hartl' *F Subject: RE: yacs *F To: 'ma11' *F X-Mailer: Mail*Link SMTP-MS 3.0.1 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='ISO-8859-1'; Name='Message Body' *F Content-Transfer-Encoding: quoted-printable *F Content-Length: 3290 *F Hi, Mike: *F Following this preamble is a brief description of the clones taken from *F the book that we have been working on. (I hope to finish it this month.) *F You will probably recognize the references as being in the EndNote format. *F I think this description will serve your needs. *F What to do about discrepant reads? I've looked quite carefully at this *F issue. For the most part the discrepancies are minor, and I have taken *F the position of listing the most conservative read (smallest region). It *F should be made clear that the reads are 'best reads' from single slides *F which do not always contain optimally stretched material. Caveat emptor *F is in order: all users should confirm the reads before using (if only to *F make sure that they haven't been sent the wrong clone)! *F One source of major discrepancy seems to be clones that contain *F transposable elements. One reader assigns a 'major site' to XXX and *F another reader assigns a 'major site' to YYY. Because of slight *F differences in slide quality, labeling intensity, etc., one preparation *F can really have a major signal at XXX and another at YYY. Another other *F source of this type of discrepancy results from chimeric clones, in which *F one reader will overlook (or choose to ignore) one of the signals and *F another reader overlook the other, so the reads are discordant. *F Again, there are not many clones in which the reads are wildly discordant *F but I think both reads should both be listed with perhaps an uncertainty *F flag of some kind. *F Description of YAC clones follows: *F The clones prefixed 'DY' are contained in the vector pYACP-1. They were *F derived from randomly sheared DNA from the Drosophila stock Oregon RC *F [Garza, 1989 #26]. *F The clones designated 'N' contain NotI fragments cloned into pYAC5 (Figure *F 2=1E8) by Andrew Link according to the method described in [Danilevskaya *F 1991 #197]. In these libraries, DNA was obtained from gastrula cells of *F Oregon RC. *F The YAC clones prefixed 'E,' 'R,' or 'RX' are in the vector pYAC4 and *F contain EcoRI fragments resulting from partial EcoRI digestion of DNA from *F a Canton=A0S stock. The 'E' clones were prepared by Elena Lozovskaya and *F the 'R' and 'RX' clones by [Cai, 1994 #1021]. *F Clones designated 'Rt' were derived from a stock of genotype T(Y;2)CB25 *F cn; y, cn bw provided by Terry Lyttle (see [Cai, 1994 #1021]). These *F clones contain EcoRI fragments in pYAC4 prepared as described in [Cai *F 1994 #1021]. *F A small number of clones, designated with the prefix 'T,' were derived *F from EcoRI partial digests of Oregon RC using the 'half-YAC' vector *F described in [Riethman, 1989 #198]. This vector yields ligation products *F lacking the right arm of the YAC, and so any DNA fragment attached to the *F left arm can rescue the YAC only if it confers genetic stability by *F serving as a telomere-addition site in yeast. The Drosophila DNA *F fragments that could rescue the 'T' clones proved not to derive from the *F tips of the chromosomes, however; upon in situ hybridzation, most of the *F clones were localized in the underreplicated mass of heterochromatin that *F aggregates to form the chromocenter or, less frequenctly, in euchromatic *F sites in the chromosome arms. *F END OF DESCRIPTION *F Best regards *F Dan # *U FBrf0075291 *a Wides *b R. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075292 *a Berg *b C. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075293 *a Salz *b H.K. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075294 *a Ayme-Southgate *b A. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075295 *a Ait-Ahmed *b O. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075296 *a Rasmuson-Lestander *b A. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075297 *a Cripps *b R. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075298 *a Schalet *b A. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075299 *a Smith *b P.D. *t 1994 *T personal communication to FlyBase *u *F FlyBase update at Chicago Drosophila meeting, April 1994. *F Copy available from FlyBase by mail # *U FBrf0075300 *a Gelbart *b W.M. *t 1994 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Fri Nov 11 20:08:18 1994 *F Date: Fri, 11 Nov 94 15:09:51 EST *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: mashburner@morgan.harvard.edu *F Subject: A home for two orphons *F Cc: adegrey@morgan.harvard.edu, gelbart@morgan.harvard.edu *F Content-Length: 3038 *F Michael, *F Two orphon abs are probably mine, although with some screw-up for one. *F Bill *F ____________ *F (1) *F \# *F \*a Df(2L)MVD1 *F \*B 23F;40 *F \*C In *F My guess is that this is really a chromosome I originally called *F In(2L)MVD1-D2. *F Mark van Doren, a technician in my lab many years ago, made a *F chromosome called Dp(2;2)MVD1. *F Dp(2;2)MVD1 is Dp(2;2) 21E ; 25A1 into 33B. *F New order: 2Lt - 33B | (21E - 25A) | 33B - 2Rt. *F \*I have conflicting notes on the orientation of the insert -- *F for now it is best to leave it as uncertain. *F The normal dpp gene on this chromosome was dpp[hr4] and *F the insertion carried dpp[d-ho]. Thus, this chromosome *F over a deletion showed transvection-sensitive complementation *F of dpp (i.e., normal wing posture). By gamma-irradiation, a *F series of derivatives that showed a heldout wing phenotype *F were recovered. *F One of these we called In(2L)MVD1-D2 (Derivative 2). It has *F an In(2L) 23F ; 40 superimposed on Dp(2;2)MVD1. The composite *F new order is: *F 2Lt - 23F | 40 - 33B | (25A1 - 21E) | 33B - 23F | 40 - 2Rt. *F Should the inversion be given a separate name, perhaps In(2L)MVD1-D2, *F and the whole entity be called 'In(2L)MVD1-D2 Dp(2;2)MVD1', *F since there are two potentially separable aberrations? *F Please also note that the current entry for Dp(2;2)MVD1 is incorrect, *F since it is called 'Dp(2;2)MVD1-2', not Dp(2;2)MVD1: *F Current gopher entry: *F \# *F Aberration symbol : Dp(2;2)MVD1-2 *F FlyBase aberration id : FBab0003484 *F Breakpoints : 21E;25A1;33B *F Class of aberration : Unoriented insertional duplication *F Aberration Reference(s) : *F FBrf0046290 == Irish and Gelbart, 1987, Genes Dev. 1: 868--879 *F FBrf0066905 == Lindsley and Zimm, 1992 *F (2) *F \# *F \*a Tp(3;2)dppd76 *F \*A dppd76 *F \*o &ggr; ray *F \*B ?;?;22F1-2 *F \*C uTp *F The cytology of this one was very difficult for me. *F What I think is likely to be going on is that there is *F an insertion of heterochromatin into a breakpoint within *F the dpp disk region. *F Is this for certain? No. *F Here's why I favor this interpretation: *F 1. Grossly, the polytenes look OK, except that: *F 2. Occasionally, a piece from 2Lt to 22F sits as free *F fragment very much as it would if there had been *F a standard translocation between 22F and centric *F heterochromatin. *F 3. 22F frequently shows ectopic fibres to telomeres, *F particularly 2Rt, to the chromocenter, as well as *F occasionally to other regions. This is unusual. *F 4. Sometimes, 22F1-2 looks unusually constricted. *F Thus, tentatively, I designated this as a new order: *F 2Lt - 22F1 | (heterochromatin) | 22F2 - 2Rt. *F If correct, there are two breaks in heterochromatin (of unknown *F origin) and a third break in 22F1-2. This should probably be *F called Dp(?;2)dpp[d76]. *F Other information: *F It is gamma-ray induced on an Oregon-R[G] chromosome. *F The discoverer is Danny Segal. *F It is a class d-III dpp disk allele. *F (In my latest stock list, I wrote that it is listed as an *F insertion of 80-80 or 81-81 into 22F1-2. However, I can't *F find any notes to suggest why I thought that.) # *U FBrf0075301 *a Boyd *b J.B. *t 1993 *T personal communication to FlyBase *u *F From jbboyd@ucdavis.edu Thu Sept 16 00:23:34 1993 *F From: jjboyd@ucdavis.edu *F To: m.ashburner@gen.cam.ac.uk *F Date: 15 Sep 93 16:14:00 PDT *F Dear Michael *F Our Genbank submission L01790 is a transcript that we sequenced while looking *F for the mei-41 gene in our chromosome walk. Internally, we call the gene *F TH1, but know nothing about its function. It is located less than *F 1 kb distal to the mei-41 transcript, and thus has the same genetic *F and cytogenetic map positions as mei-41 (54.2, 14C4-6). It potentially *F codes for a 43 kDa protein. *F Paul Harris, for Jim Boyd. # *U FBrf0075302 *a Belote *b J.M. *t 1993 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: John Belote, Syracuse University *F To: Bloomington Drosophila Stock Center *F Subject: Df(3L)st7 breakpoints *F Date: 26 October 1999 *F *F Background: There is a good deal of confusion over the breakpoints of Df(3L)st7. The Lindsley & Zimm lists them as 73A3-4;74A3, but cites Belote's Cell 48:489 paper, which describes Df(3L)st7P and Df(3L)stj7, but not Df(3L)st7. Ashburner's aberrations file on FlyBase gives the breakpoints as 73A3-4;74A3-4 and cites Tearle et al. (1989) Genetics 122:595. These are the breakpoints Tearle et al. give for this deficiency, but they cite DIS 65 (the rearrangements section of the new redbook in progress) and Velissariou & Ashburner (1981) Chromosoma 84:173, which give the breakpoints of Df(3L)st7 as 72F3-4;74C3-4 and 72F4;74C2-3, respectively. John was asked if he could clarify. *F *F Information communicated: *F *F "I went back and looked in my old notebooks from 1983 when I did a lot of cytology on the known st deficiencies and I found my drawings of several salivary chromosome squashes of this deficiency. My conclusion at that time, based on what look to be some pretty good squashes, was that the breakoints were as stated in the Tearle, et al paper: 73A3.4 - 74A3. However, I never did anything to genetically test whether or not this determination was correct. I'm pretty sure that the 74A4.5 doublet is not deleted, but it is possible that the lefthand breakpoint is in 73A1.2 instead of 73A3.4." *F *F # *U FBrf0075303 *a Duncan *b I. *t 1993 *T personal communication to FlyBase *u *F Fax from Ian Duncan, 3 September 1993 *F Copy available from FlyBase by mail # *U FBrf0075304 *a Hickey *b D. *t 1993 *T personal communication to FlyBase *u *F email to M. Ashburner 2 September 1993 *F Copy available from FlyBase by mail # *U FBrf0075305 *a Rushlow *b C.A. *t 1993 *T personal communication to FlyBase *u *F fax to M. Ashburner 7 September 1993 *F Copy available from FlyBase by mail # *U FBrf0075306 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Nov 14 18:43:36 1994 *F X-Sender: jr32@mole.bio.cam.ac.uk (Unverified) *F Mime-Version: 1.0 *F Date: Mon, 14 Nov 1994 18:40:42 +0000 *F To: Michael Ashburner *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: JR says *F Content-Length: 8745 *F \*a Dp(2;Y)b88b15 *F \*B 34D1-34D2;35E2-35E5;Y *F \*C uDp *F \*G rk << bk1 << ? << bk2 *F JR says: the Dp segregant from Alexandrov's Tp(2;Y)b88b15 (called *F Df(2L)b88b15 in Alex and Alex 1991 DIS 70:19, but he noted that the Df was *F 'shorter in males than in females'). Alex's cytology: 34D1-2;35E2-5. MA's *F cytology of the Df segregant: 34C3;35F2-12? (the ? is MA's). The Dp has *F never been separated but is inferred from the difference in mapping the Df *F from males and females. It has not been published. *F \# *F \*a DS(2)B1 *F \*B 35B;44C *F \*C A *F JR says: made by Gubb, not published, lost. *F \# *F \*a DS(2)TE35B-50 *F \*B 35B;41 *F \*C A *F \*i DS(2)TE146(Z)GV50 *F JR says: cytology correct - autosynaptic made by Terri Morley - not published. *F \# *F \*a In(2L)C163LTE35B-210R *F In(2L)C163.41LTE146(Z)GR210R *F JR says: recombinant (by JR) from C163.41/GR210, picked up as E(H), *F sterile. presumably Dp 35B-35E, Df 27D-28D, to be published in Roote et al. *F \# *F \*a In(2LR)b71k1A *F \*B 34C7;34D6-34D7 *F \*C Df *F \*G l(2)34Db << bk1 << Sos << b << bk2 << l(2)34Dc *F JR says: The original cytology published by Alex was:- *F Aberration symbol : Tp(2;2)b[71k1] *F Synonym(s) : Tp(2;2)b71k1 *F Full name : Transposition (2;2) black *F FlyBase aberration id : FBab0009774 *F Breakpoints : 34D2-4;34D8-E1;43C2-4 *F Class of aberration : Unoriented insertional transposition *F Associated allele : FBal0000873 == b[71k1] *F Revised cyto. map posn. : Tp:34D2-34D4:34D8-34E2:43C2-43C4 *F Genetic information : *F Mutant for b but not for nub or j. Homozygous lethal. *F Mutagen/how isolated : gamma ray induced. *F Aberration Reference(s) : *F FBrf0044054 == Alexandrov and Alexandrova, 1986, D. I. S. 63: 159--161 *F FBrf0044068 == Alexandrova, 1986, D. I. S. 63: 21 *F FBrf0066905 == Lindsley and Zimm, 1992 *F The first stock of 71k1 that he sent to us was In(2L)34D4;34D6.7 (MA) and *F homozygous viable. He sent us another copy of 71k1 a few years later that *F is now called In(2LR)b71k1A and is mutant for Sos and b The cytology of *F this new stock is In(2LR)34D4-8;43C1-4 (MA). We therefore tried v hard to *F make an autosynaptic with SZ4 and failed completely. However 71k1A does *F have a lesion in 43 (probably l(2)43Bc - we are checking now). *F \# *F \*a In(2LR)b81k1A *F \*B 34D4;43C2 *F \*C In *F JR says: typo - should be 71k1A *F \# *F \*a In(2LR)D20LD32R *F \*c Df *F \*G j << bk1 << rk << l(2)34Fa << bk2 << wb *F JR says: LS(2)D20 and DS(2)D32 both published in Gubb1988 Genetics *F 119:377-390. David made the close-up, which is deleted for rk - 34Fa. Not *F published. *F \# *F \*a In(2LR)Scorv1-DV11 *F \*B 35D1-35D2;40-41;44C3-44C5 *F \*C cIn *F \*O In(2LR)Scorv1 *F \*i In(2LR)ScoR+1-DV11 *F JR says: gamma-induced revertant - cytologically like rv1 - will be *F published in Roote et al. 1994 Genetics. *F \# *F \*a In(2LR)TE35B-300 *F \*B 35B;43A1-43A2 *F \*C In *F \*G l(2)34Fa << bk1 << wb *F \*i In(2LR)TE146(Z:SR14)GR300 *F JR says: gamma-induced red-eyed derivative from SR14 (zesty-eyed), deleted *F for wb-noc, published in Roote et al. and L & Z. *F \# *F \*a In(2LR)TE35B-301 *F \*B 35B;42F1-42F4 *F \*C In *F \*i In(2LR)TE146(Z:SR14)GR301 *F JR says: similar to GR300, mutant for wb (but not a deletion), not published. *F \# *F \*a In(2LR)TE35B-4LTE35B-14R *F \*B 42E7;43A3-43A4 *F \*G bk1 << nec << pk << bk2 *F \*i In(2LR)TE146(Z:SR36)SZ4LTE146(Z)SR14R *F JR says: mutant for noc, duplicated for nec and pk, from auto *F intermediates. Not published, but might be in the paper on genetics of *F 42-43. *F \# *F \*a T(2;3)H16DTE35B-3aP *F \*i T(2;3)H16DTE146(Z)GR3P *F JR says: by segregation, by JR, duplicated for 35B2;35D7, deleted for *F 85F6.8;86F12-87A1.2, carries w+rst+, published in Roote et al. and L & Z. *F (H16 renamed G16). *F \# *F \*a T(2;3)H40DTE35B-28P *F \*c Duplicated for 35B2;35F4-35F5 and 90C3-90C4;91E5-91E6 *F \*i T(2;3)H40DTE146(Z)GR28P *F JR says: by segregation, by JR, carries w+rst+, published in Roote et al. *F (H40 renamed G40) *F \# *F \*a T(2;4)DTD22DTE35B-50P *F \*i T(2;4)DTD22DTE146(Z)GR50P *F JR says: by segregation, by JR, duplicated for 35B1.2;35E1.2, carries w+ *F rst+, published in Roote et al. and L & Z. *F \# *F \*a T(2;4)TE35B-101DGT6P *F \*c Dp *F \*G wb << bk1 << l(2)34Fc << noc << bk2 << osp *F \*i T(2;4)TE146(Z:SR100)GV101DGT6P *F JR says: duplicated 34Fc--noc, published in Roote et al. *F \# *F \*a T(Y;2)el4DA80P *F \*B 35A2-35A3;35A3-35A4 *F \*C Df *F \*G elA << bk1 << pu << bk2 << elA *F JR says: by seg., deleted for elB--pu, in Roote et al. *F \# *F \*a T(Y;2)el4DR15P *F \*B 35A2-35A3;35B8-35C1 *F \*C Df *F \*G elA << bk1 << pu << l(2)35Bg,Su(H) << bk2 << ck *F JR says: by seg., deleted for elB--35Bg, in Roote et al. *F \# *F \*a T(Y;2)J165DP58P *F \*B 35C4-35C5;35D5-35D7 *F \*C Df *F JR says: dunno {I do ma!, segregation; we made it once but *F did not do much with it] *F \# *F \*a T(Y;2)R15DJ165P *F \*B 35B9-35C1;35C4-35C5 *F \*C Df *F \*G l(2)35Bg,Su(H) << bk1 << ck << dgl << bk2 *F JR says: dunno [ibid] *F \# *F \*a T(Y;2)R15DTE35B-18P *F \*B 35B1-35B2;35B9-35C1 *F \*G noc << bk1 << noc << l(2)35Bg,Su(H) << bk2 << ck *F \*i T(Y;2)R15DTE146(Z)GR18P *F JR says: by segregation, by JR, duplication 35B1.2;35B9-C1 (noc--35Bg), *F carries w+ rst+, published in Roote et al. *F \# *F \*a T(Y;2)TE35B-18DA80P *F \*c Dp *F \*i T(Y;2)TE146(Z)GR18DA80P *F JR says: by seg, by JR, duplicated for elA, does not carry w+ or rst+, published in Roote et al. *F \# *F Department of Genetics *F University of Cambridge *F Downing Street *F Cambridge *F CB2 3EH *F UK *F Tel: 44 223 333982 *F Fax: 44 223 333992 *F email: j.roote@gen.cam.ac.uk # *U FBrf0075307 *a Kania *b A. *t 1994 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Arthur Kania, Baylor College of Medicine *F To: Bloomington Drosophila Stock Center *F Subject: Df(2R)Jp6 breakpoints *F Date: 14 November 1994 *F *F Information communicated: *F "I just spoke to Bill Saxton about the jp deficiencies @ 52A-F. I noticed a discrepancy between the breakpoints listed in flybase and *F those in his paper (Cell 64:1093 Fig.1). Jp6 is shown to be 52E3-5; 52F, whereas in flybase it's listed as 52D3; 52F10-11. He confirmed *F that the Df breakpoint in the paper is the correct one." # *U FBrf0075308 *a Lyttle *b T.W. *t 1994 *T personal communication to FlyBase *u *F From tlyttle@uhunix.uhcc.hawaii.edu Mon Nov 14 22:16:35 1994 *F Date: Mon, 14 Nov 1994 12:14:47 -1000 *F From: Terrence Lyttle *F To: ma11 *F Cc: *F Subject: Re: Help FlyBase please *F Content-Length: 494 *F Michael, *F SD-NH2 is in fact a second isolate of SD-NH collected by Hiraizumi and *F Nakazima at the same time as their 1965 DIS note. I got it from the Crow lab *F donkey's years ago. SD-79 is the same structural SD chromosome as SD5 and *F SD36, collected from nature (Jim Crow's back yard) in 1979, whence its name. *F It is an independent isolate, but since it carries the SD5 inversions, maybe *F the best way to crosslist it in the RedBook is to refer to SD5 or SD36. *F Cheers, *F Terry # *U FBrf0075309 *a Knust *b E. *t 1993 *T personal communication to FlyBase *u *F From EKNUST@biolan.uni-koeln.de Fri Jul 9 15:38:43 1993 *F Date: Fri, 9 Jul 1993 16:38:54 +0200 (WET-DST) *F From: EKNUST@biolan.uni-koeln.de (Elisabeth Knust) *F Subject: message from Eli *F To: rd120@gen.cam.ac.uk *F X-Vmsmail-To: SMTP%'rd120@gen.cam.ac.uk' *F Content-Length: 723 *F Status: RO *F X-Lines: 11 *F Dear Rachel, this letter concerns your request on Esm. this mutation has *F been isolated in a screen for dominant enhancers of split. It maps *F roughly to position 3-81, and is not repressed by Dp(3;3)SuM(3)w13. It *F has been induced by hybrid dysgenesis. It carries two P-elements in *F 95F, but it is not known which one is the cause of the mutation. Most *F results support the assumption that it is not allelic to crumbs, but *F we have not isolated any revertants of this dominant mutations. If you need *F further information, please let me know. I also have a question concerning *F your work on the Flybase project: is it possible to get some information *F about mutations by e-mail? and if so, how is the procedure? *F Best wishes, Eli. # *U FBrf0075310 *a Schafer *b U. *t 1993 *T personal communication to FlyBase *u *F Letter from U. Schafer to FlyBase, 14 June 1993 *F Copy available from FlyBase by mail # *U FBrf0075311 *a Wolfner *b M.F. *t 1993 *T personal communication to FlyBase *u *F Letter from M. Wolfner to Flybase, 24 June 1993 *F Copy available from FlyBase by mail # *U FBrf0075312 *a Pak *b W.L. *t 1993 *T personal communication to FlyBase *u *F Letter from W. Pak to FlyBase, 18 June 1993 *F Copy available from FlyBase by mail # *U FBrf0075313 *a Santamaria *b P. *t 1993 *T personal communication to FlyBase *u *F Fax from P. Santamaria to FlyBase, June 1993 *F Copy available from FlyBase by mail # *U FBrf0075314 *a Edgar *b B.A. *t 1994 *T personal communication to FlyBase *u *F From bedgar@fred.fhcrc.org Wed Jan 19 05:02:59 1994 *F Date: Tue, 18 Jan 94 20:59:20 PST *F To: ma11@gen.cam.ac.uk *F From: bedgar@fred.fhcrc.org *F Subject: D69 clone/flybase *F Content-Length: 622 *F Status: RO *F X-Lines: 16 *F Dear Michael, *F Sorry to confuse you about the identity of the D69 sequence we submitted to *F genebank. This is a fly cDNA clone that I isolated in Paul Nurse's lab by *F virtue of its ability, when overexpressed, to arrest fission yeast cells in *F a mitotic-like state. All we have is the clone and the sequence, which was *F uninformative since it is not very homologous to anything in the database. *F We have not yet mapped it to polytenes or studied expression or function in *F Drosophila. It's not a high priority in my lab at present. *F Thanks for your query; I'm sorry I cannot clarify D69's identity. *F Best wishes, *F Bruce Edgar # *U FBrf0075315 *a Fyrberg *b E. *t 1993 *T personal communication to FlyBase *u *F Fax from E. Fyrberg to FlyBase 6 December 1993. *F Copy available from FlyBase by mail # *U FBrf0075316 *a White *b R. *t 1993 *T personal communication to FlyBase *u *F Record of telephone conversation with Rob White 16 December 1993 *F Copy available from FlyBase by mail # *U FBrf0075317 *a O'Hare *b K. *t 1994 *T personal communication to FlyBase *u *F From k.ohare@ic.ac.uk Mon May 2 14:11:19 1994 *F X-Sender: kohare@bccmsa.bc.ic.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 02 May 1994 14:01:08 +0100 *F To: flybase@morgan.harvard.edu *F From: k.ohare@ic.ac.uk (K.O'Hare) (K.O'Hare) *F Subject: Corrections for entry in flybase/genes *F X-Mailer: *F Content-Length: 1140 *F Status: RO *F X-Lines: 22 *F Congratulations on doing such a good job on the entry for suppressor of *F forked in flybase/genes. I did spot a couple of errors, so here are some *F suggested corrections/additions: *F 1.Molecular biology data: Add reference describing the cloning of the gene - *F Mitchelson, Simonelig, Williams, O'Hare (1993) Genes & Development *F 7:241-249. Also add that major predicted protein product is homologous (25% *F identical, 47% similar) to RNA14 of Saccharomyces cerevisiae which has a *F role in RNA stability. *F 2. Phenotypic information for allele su(f)[3]: it is stated that su(f)[3] *F fails to complement 'either su(f)[3] or su(f)[5]'. I suspect it should read *F 'either su(f)[2] or su(f)[5]'. *F 3. Reference for su(f)[27] and su(f)[28] is incorrect. It should be Simmons, *F Raymond, Johnson, Fahey (1984) Genetics 106:85-94. *F Kevin O'Hare Tel: 44 + 71 225 8267 *F Department of Biochemistry Fax: 44 + 71 225 0960 *F Imperial College of Science, Technology & Medicine *F London SW7 2AZ. # *U FBrf0075318 *a Spradling *b A.C. *t 1994 *T personal communication to FlyBase *u *F From spradling@mail1.ciwemb.edu Wed Mar 9 19:04:02 1994 *F Date: 9 Mar 1994 14:02:41 +0000 *F From: 'Allan Spradling' *F Subject: Re: Pelement data *F To: 'ma11' *F Content-Length: 8924 *F Status: RO *F X-Lines: 212 *F We don't have any record of the following aberrations in either L&Z or *F our files - any data in L&Z style would be very welcome *F Df(2L)TE146X1 = Df(2L)TE196X1 [S. Sugiyama] *F Df(2)03072 =Df(2R)51A5-C1 [T. Laverty] *F Df(2L)PM101 =Df(2L)25E01,02-26A02,05 [R. MacIntyre] *F Df(2R)02311 =Df(2R)58D2-E1 [T. Laverty] *F Df(3)01215 =Df(3R)99A6-C1 [T. Laverty] *F Df(3)04661 =Df(3R)100D2-F5 [T. Laverty] *F Df(3L)ZN47 =3096 Df(3L)ZN47, ry[506]/TM3, ri p[p] 064C;065C *F sep ry su(Hw)[2] bx[34e] Sb e (Bloomington) *F Df(3L)h-i22ry506 =Df(3L)66D10,11-66F01,02 [source unknown] *F Df(3L)ri-XT1 = Df(3L)77E02-78A01,02 [from R. Kelley, source unknown] *F Df(3R)01215 see above *F Df(3R)B81P =3546, 'Df(3R)B81, P{ry[+]-rp49}F2-80A e/TM3; Dp(3;1)67A','3','099C08;100F05, 099D;100F (Dp), 080A','John Merriam, 1989; Nature 317:555-558, 1985' *F Df(3R)P115 = 1467 Df(3R)P115, e[11]/TM1; Dp(3;1)P115 089B07-08;089E07-08;020 *F = Tp(3;1)P115 *F Df(3R)P712 = 1968 Df(3R)p712, T(2;3)p712, red e/TM3 084D04-06;085B06, 025D;085B06 *F Df(3R)R133 = 2234 Df(3R)R133, B[S]/TM3; Dp(3;1)124P 099E;100F, 099E04-05;100F05 *F Df(3R)awd-KRB = 3369 Df(3R)awd-KRB, ca/TM3, y[+] Sb e 100C-D *F Df(3R)hhE23 =3644 ,'Df(3R)hhE23, ru h th st cu sr e[s] ca/TM3','3','094;095 (094A;095A?)','Joan Hooper, 2/91; gamma ray induced' *F Df(3L)81k19 =2998, 'Df(3L)81K19/TM6B','3','073A03;074F','' # *U FBrf0075319 *a Crowley *b T. *t 1994 *T personal communication to FlyBase *u *F Letter from T. Crowley to FlyBase, 18 January 1994 *F Copy available from FlyBase by mail # *U FBrf0075320 *a O'Hare *b K. *t 1994 *T personal communication to FlyBase *u *F From k.ohare@ic.ac.uk Wed Jun 1 13:38:54 1994 *F X-Sender: kohare@bccmsa.bc.ic.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 01 Jun 1994 13:24:25 +0100 *F To: eleanor *F From: k.ohare@ic.ac.uk (K.O'Hare) (K.O'Hare) *F Subject: : p$p25.7bwc construction *F X-Mailer: *F Sender: k.ohare@ic.ac.uk *F Content-Length: 2069 *F Eleanor, *F The plasmid p$p25.7bwc was made it independently of that made by Frank Laski *F as follows: *F p$p25.7 has a 4.7 kb BamHI fragment inserted into the BamHI site of pBR322. *F The insert is from 17C in the strain $p2 and includes a complete 2.9 kb P *F element. The complete sequence of the insert is in the nucleic acid *F databases. This is the same insert as in p$p25.1, one of the very first *F providers of P transposase for transformation, except that the orientation *F of the insert is reversed. In p$p25.7, the right end of the P element is *F closest to the SalI site ofpBR322. Roger Karess then made p$p25.7wc by *F inserting a SalI linker at the AvaII site in the right inverse repeat, and *F dropped out the sequences flanking the P element on the right by cutting *F with SalI and re-sealing the circle. I then took p$p25.7wc, the first 'wings *F clipped' helper, and dropped out the sequences flanking the P element on the *F left by partially digesting with HindIII and re-sealing, so that the *F derivative p$p25.7bwc 'both wings clipped' has no flanking sequences, and is *F missing only the first 40 bp and last 25 bp (approx) of the complete 2.9 kb *F P element. I use it as a probe for P elements, but I have never tried to use *F it to provide transposase. As the promoter region has been altered, it may *F well be expressed at a lower level than p$p25.7wc, and has no advantages *F over this or other helpers. *F The reference for this is *F TI: DISTRIBUTION AND STRUCTURE OF CLONED P-ELEMENTS FROM THE *F DROSOPHILA-MELANOGASTER P-STRAIN PI *F AU: OHARE_K, DRIVER_A, MCGRATH_S, JOHNSONSCHILTZ_DM *F JN: GENETICAL RESEARCH 1992 VOL.60 NO.1 PP.33-41 *F If this description is unclear, I have a map showing all these related *F plasmids, and if you give me a Fax number, I'll send it to you. *F Kevin *F -------------------------------------------------------------------------------- *F Kevin O'Hare Tel: 44 + 71 225 8267 *F Department of Biochemistry Fax: 44 + 71 225 0960 *F Imperial College of Science, Technology & Medicine *F London SW7 2AZ. # *U FBrf0075321 *a Blackman *b R. *t 1994 *T personal communication to FlyBase *u *F From ron_blackman@qms1.life.uiuc.edu Thu Jul 7 00:53:56 1994 *F Date: 6 Jul 1994 18:44:54 -0500 *F From: 'Ron Blackman' *F Subject: Re: Inversion name, carries *F To: 'eleanor' *F X-Mailer: Mail*Link SMTP/QM 3.0.0GM *F Content-Length: 2019 *F Reply to: RE>Inversion name, carries Hobo *F See info below. Write again if you have questions. Ron *F -------------------------------------- *F Date: 7/1/94 7:44 AM *F To: Ron Blackman *F From: eleanor *F Dear Ronald, *F I am a member of FlyBase at the Cambridge, England site and I am *F currently working on the curation of constructs and vectors. Before *F curating any recent journals I am having to return to the original *F papers where the most detail about vector construction is given. In *F your paper about the identification of a fully functional hobo *F transposable element (Embo J. 8: 211--217) you discuss an inversion *F between dpp and an unknown locus in region 22E1--22E2. Unfortunately *F you do not name this. *F While searching my aberrations file I found: *F ab symbol : In(2L)dpp[s5] *F ab name : Inversion (2L) decapentaplegic *F reference : Gelbart in Lindsley and Zimm, 1992 *F reference : Gelbart *F cytology : 21E1-21E2;22F1-22F2 *F mutagen : X-ray *F I realise the first breakpoint is wrong but it is the nearest inversion *F description I could find to the one you used. Is this the one? *F If not could you please give me a complete description so I can *F incorporate it into FlyBase. I need the inversion to give a progenitor *F chromosome for the BamHI HindIII fragment that carried the Hobo element. *F The information I require: *F Inversion name: In(2L)dpp^d1 (that's superscripted d1--the very first dpp *F allele Bill gelbart recovered--It's in L & Z.) *F Inversion cytology: see L & Z *F Has the unknown locus at 22E1--22E2 been named? Not to my knowledge *F Which breakpoint is within the BamHI HindIII fragment? Breakpoint is at *F 105.0 on the dpp molecular map. Har1 contains the 105 to 106.3 sequences of *F dpp (that is, the sequences centromere proximal of the breakpoint). I hope *F this answers your question. *F 21 ? dpp ? dpp 60 *F --------+----------+----------- *F ^ or ^ *F | | *F Is the Hobo insertion absolutely at the inversion breakpoint? Yes *F Thank you very much, *F Eleanor Whitfield. # *U FBrf0075322 *a Pirrotta *b V. *t 1994 *T personal communication to FlyBase *u *F From pirrotta%sc2a.unige.ch@mhs-relay.ac.uk Wed Jul 13 09:11:03 1994 *F Date: Wed, 13 Jul 1994 10:03:52 +0100 *F X400-Originator: pirrotta@sc2a.unige.ch *F X400-Recipients: eleanor@gen.cam.ac.uk *F X400-Mts-Identifier: [/PRMD=SWITCH/ADMD=ARCOM/C=CH/;<01HENJPI3A8I002VP2@uni2b.unige.] *F X400-Content-Type: P2-1984 (2) *F Content-Identifier: vectors *F From: pirrotta@sc2a.unige.ch *F To: eleanor@gen.cam.ac.uk *F Subject: vectors *F X-Envelope-To: eleanor@genetics.cambridge.ac.uk *F Content-Transfer-Encoding: 7BIT *F Content-Length: 1530 *F dear Eleanor *F sorry. The Cahsneo vector you refer to is only fleetingly described in the *F article: *F Pirrotta, V. (1988) Vectors for P-mediated transformation in Drosophila. *F In: Vectors. A survey of molecular cloning vectors and their uses. Eds. *F R.L. Rodriguez and D.T. Denhardt. Butterworths. Boston. pp. 437-456. *F The brief description of Cahsneo and some vectors based on it are on p. 445 *F and p. 448. The Cahsneo with hs43-lacZ is described (also sketchily) in: *F Qian, S., Varjavand, B. and Pirrotta, V. (1992) Molecular analysis of the *F zeste-white interaction reveals a promoter-proximal element essential for *F distant enhancer-promoter interaction. Genetics 131:79-90 *F It contains not the full hsp70 promoter but just the TATA box, with *F sequences upstream of -43 deleted. *F The article in the Vectors book describes the cosPneo vector but concerning *F the cosPer vector, it says only that a cosmid version of CaSpeR is also *F available (cosPer). I am afraid that it has never been described any *F better than that. However, what it amounts to is just the cosPneo with the *F hsp70-neo gene replaced by the same mini-white gene that is present in *F CaSpeR. *F Sorry about this inadequate answer but the details have never been *F published. Journals do not like to publish papers on such thing these *F days. And rightly so. But there is no other institutionalized way to make *F the information accessible and quotable. *F V. Pirrotta *F Dept. of Zoology, Sciences 3 *F University of Geneva *F 30, quai E. Ansermet *F CH-1211 Geneva, Switzerland # *U FBrf0075323 *a Zuker *b C.S. *t 1994 *T personal communication to FlyBase *u *F From charles@flyeye.ucsd.edu Thu Aug 18 02:57:54 1994 *F Date: Wed, 17 Aug 94 18:51:46 PDT *F From: charles@flyeye.ucsd.edu (Charles Zuker) *F To: eleanor@gen.cam.ac.uk *F Subject: P element structure *F Content-Length: 389 *F Dear Eleanor, *F Sorry for the delay in answering. It took me a while to go back to the *F data in Coen (Genetics 126:949). According to my records (from 1984!!) the *F white duplication was inserted into the carnagie3 vector of Rubin and Spradling. *F This is the vector described in Nucleic Acids Research 11:6341 (1983). *F best wishes *F charles zuker # *U FBrf0075324 *a Gelbart *b W.M. *t 1994 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Thu Nov 17 14:58:01 1994 *F Date: Thu, 17 Nov 94 09:59:36 EST *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: ma11@gen.cam.ac.uk *F Subject: Re: pers comm *F Cc: gelbart@morgan.harvard.edu *F Content-Length: 1095 *F Michael, *F Since I'm not certain if I'd be appropriately using the * codes, *F I won't try. *F Df(2L)dpp[d33] arose in a P-M hybrid dysgenesis screen for *F mutants allelic to dpp[d-ho], carried out by Mike Hoffmann. *F The deletion breakpoints are 22F1-22F2;23A1-23A2 *F as you note. Southern and in situ analysis show no evidence *F of a P element at the deletion junction and suggest that it *F is similar (if not identical) to Df(2L)dpp[d14] in extent. *F Genetically, Df(2L)dpp[d33] is a class V disk allele. In addition, *F it removes l(2)22Fb, l(2)22Fc and l(2)22Fd. *F Hope this is what you wanted. *F Bill *F From gelbart@morgan.harvard.edu Thu Nov 17 20:41:33 1994 *F Date: Thu, 17 Nov 94 15:43:10 EST *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: ma11@gen.cam.ac.uk *F Subject: Re: pers com *F Cc: gelbart@morgan.harvard.edu *F Content-Length: 212 *F >does this imply that d14 also removes l(2)22Fb, l(2)22Fc and l(2)22Fd? *F yup *F bill # *U FBrf0075325 *a Mann *b R. *t 1994 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Tue Dec 6 18:28:39 1994 *F From: Michael Ashburner (Genetics) *F Date: Tue, 6 Dec 94 18:29:48 GMT *F To: mann@cuhhca.hhmi.columbia.edu *F Subject: Help FlyBase *F Cc: ma11@gen.cam.ac.uk *F Content-Length: 903 *F Richard Mann *F Dear Richard *F FlyBase has these rather enigmatic records. It is now our policy not *F to include data in the database that we cannot jistify by a written or *F email record. These are then archived and made available to users. *F Could you either confirm or deny these data - or would you rather *F they were deleted ? Are they published ? is there anything you would now *F wish to add ? If affirmative we will archive yr reply as a FB personal *F communication and reference this from the record. *F Michael *F \*a belt *F \*z FBgn0005635 *F \*e belt *F \*H Last updated 13 Nov 94 *F \*b 2-[24] *F \*c 28E--28F *F \*x Mann, 1992, personal communication (Crete meeting) *F \# *F \*a lip *F \*z FBgn0005637 *F \*e lips *F \*H Last updated 14 Nov 94 *F \*b 3-[47.1] *F \*c 82E *F \*x Mann, 1992, personal communication (Crete meeting) *F \# *F \*a nvy *F \*z FBgn0005636 *F \*e nervy *F \*H Last updated 14 Nov 94 *F \*b 3-[101] *F \*c 99F *F \*x Mann, 1992, personal communication (Crete meeting) *F \# *F From mann@cuhhca.hhmi.columbia.edu Tue Dec 6 18:42:30 1994 *F Date: Tue, 6 Dec 94 13:35:18 -0500 *F From: mann@cuhhca.hhmi.columbia.edu (Richard Mann) *F To: ma11@gen.cam.ac.uk *F Subject: Re: Help FlyBase *F Content-Length: 588 *F Dear Michael, *F A manuscript describing the initial isolation and mapping of these genes *F has recently been submitted for publication and, with the help of the *F reviewers, should be in press soon. This paper also includes alot more *F data on nvy, including its complete sequence. We also have alot of *F additional data on nvy and lips (including its complete sequence) which *F is not in this paper, but which hopefully will be submitted sometime in *F 1995. So my feeling is to keep the flybase entries as is and that they *F can be updated as these papers are (hopefully) published. *F Regards, *F Richard # *U FBrf0075326 *a Salz *b H.K. *t 1994 *T personal communication to FlyBase *u *F From hks@thor.ins.cwru.edu Tue Dec 6 19:00:09 1994 *F Date: Tue, 6 Dec 1994 13:59:30 -0500 *F To: ma11 *F From: hks@thor.INS.CWRU.Edu (Helen Salz) *F Subject: Re: Help FlyBase *F Content-Length: 853 *F Dear Michael, *F Please purge from your files those two very enigmatic records. Although *F these stocks do exist, I have never had the time to fully analyze them. *F Maybe in another life I will. *F I do have some info that you might find interesting, however. I have *F oriented the genetic and molecular maps in the snf region (4F1,2 by insitu *F hybridization). The order is dital, snf, dhd , l(1)4Fb, fs(1)1059. *F fs(1)1059 is outside of my walk, the other genes are within my walk. The *F only allele not published yet is l(1)4Fb. *F Hope all is well in sunny england.....are you coming to Atlanta? See you there. *F Helen *F The two enigmatic records to be deleted are: *F > *F >*a In(1)5015 *F >*x Salz, 1988, personal communication, Crete meeting *F >*B 4C11-4C16;[] *F >*C In *F ># *F >*a In(1)6678 *F >*x Salz, 1988, personal communication, Crete meeting *F >*B 4C11-4C16;[] *F >*C In *F ># # *U FBrf0075327 *a Gampel *b A. *t 1994 *T personal communication to FlyBase *u *F From AG138@phx.cam.ac.uk Fri Dec 9 12:14:14 1994 *F Date: Fri, 09 Dec 94 12:13:37 GMT *F From: AG138@phx.cam.ac.uk *F To: ma11 *F Subject: Re: puc update *F Content-Length: 1464 *F Michael, *F The paper we are now putting together will include the cDNA sequence, a map *F of the genomic region, location of the P elements (see below) in puc and *F breakpoints in revertants of one of the P element lines. Tell me if you *F want any of this information prior to publication. *F I don't understand the cytological location listed as 84E11. To the best *F of my understanding, it is 84Eh as indicated in Baker et al 1991. *F Although the puc[1] (K10) and puc[3] (k36) alleles are described in the *F Baker paper, I have been told by Alfonso that they were lost at the source. *F There are three lethal P element lines with P element insertions in puc: *F pucE69 (Ring and AMA, 1993) is embryonic lethal, with puckered dorsal *F midline and overall boat shape. This line contains placA92 (O'Kane and *F Gehring, 1987. PNAS 84;9123-9127) *F pucA251.1 (Bellen et al,1989. Genes and Dev. 3;1288-1306) contains plArB *F (also Bellen et al). This is also embryonic lethal with a stronger *F phenotype than pucE69, but note that pucA251.1 contains a second site *F mutation. *F puc320c (a gift from Jose Campos Ortega, unpublished as far as I know) *F contains P-lacW (Bier et al, 1989. Genes and Dev. 3;1273-1287). It is *F a polyphasic lethal. *F As the only reference to puc as vco is word of mouth and the first *F edition of the blue bible, do you think we should keep it in as a *F synonym? I'm not bothered either way. *F I hope this is what you need. If there's anything else please tell me. *F Cheers, *F Alexandra Gampel # *U FBrf0075328 *a Hilliker *b A. *t 1994 *T personal communication to FlyBase *u *F From ahillike@uoguelph.ca Fri Dec 9 20:45:18 1994 *F Date: Fri, 9 Dec 1994 15:45:54 -0400 (EDT) *F From: Arthur J Hilliker *F Subject: *F To: eleanor@gen.cam.ac.uk *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 123 *F The correct breakpoint is 42A3-19 as you thought. (reference: *F T(2;3)6rl). *F A.J. Hilliker *F Molecular Biology and Genetics # *U FBrf0075329 *a Romani *b S. *t 1994 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Mon Dec 12 10:18:18 1994 *F From: Michael Ashburner (Genetics) *F Date: Mon, 12 Dec 94 10:19:38 GMT *F To: sr10008@phx.cam.ac.uk *F Subject: what you told aubrey *F Cc: ma11@gen.cam.ac.uk *F Content-Length: 1322 *F This is what we have in FlyBase from what you told Aubrey: *F \*a ASC-T1 *F \*z FBgn0011757 *F \*e achaete-scute-complex transcript 1 *F \*H Last updated 13 Nov 94 *F \*i T1 *F \*x FBrf0057717 == Campuzano and Modolell, 1992, Trends Genet. 8(6): 202--208 *F \*x Modolell and Romani, personal communication to FlyBase, June 1994 *F \*u A transcription unit in the achaete-scute complex, lying just proximal *F \*u to ase. It is transcribed from the opposite strand to all four of the *F \*u genes normally included in the complex, ie 5'--3' = proximal--distal. *F \*u Its function is unknown. *F \*U Stage 16 onwards in the tracheal tree. *F \# *F \*a pcl *F \*z FBgn0011822 *F \*e pepsinogen-like *F \*H Last updated 14 Nov 94 *F \*i T2 *F \*i sc&bgr; *F \*x FBrf0057717 == Campuzano and Modolell, 1992, Trends Genet. 8(6): 202--208 *F \*x FBrf0046281 == Dambly-Chaudiere and Ghysen, 1987, Genes Dev. 1(3): 297--306 *F \*x Modolell and Romani, personal communication to FlyBase, June 1994 *F \*u A transcription unit in the achaete scute-complex, lying between l(1)sc *F \*u and ase. It is transcribed from the opposite strand to all four of the *F \*u genes normally included in the complex, ie 5'--3' = proximal--distal. *F \*u Its function is poorly understood. *F \*U Posterior midgut from about stage 15. *F \# *F We DO NOT want to have information in FlyBase which is by word of mouth ! *F Michael *F From SR10008@phx.cam.ac.uk Mon Dec 12 10:36:36 1994 *F Date: Mon, 12 Dec 94 10:35:56 GMT *F From: SR10008@phx.cam.ac.uk *F To: ma11 *F Subject: Re: [what you told aubrey] *F Content-Length: 427 *F What I told him is that the so called T2 transcript of the AS-C codes for a putative 406 amino acids protein which shows striking homology with the family of aspartic acid protease precursors. The best fit is with the swine pepsinogen A precursor. That is why Juan suggested the name of Dpsl (Drosophila pepsinogen-like *F protein). I also told him the data under ASC-T1. *F All these are unpublished data and we have the paper half ready. Is it all right? Chusi. Susana. # *U FBrf0075330 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Dec 19 09:40:15 1994 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 19 Dec 1994 09:39:28 +0000 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: 7-10-5 *F Content-Length: 1164 *F >----- Begin Included Message ----- *F > *F >>From jr32@mole.bio.cam.ac.uk Wed Nov 16 16:15:31 1994 *F >Mime-Version: 1.0 *F >Content-Type: text/plain; charset='us-ascii' *F >Date: Wed, 16 Nov 1994 16:14:05 +0000 *F >To: Michael Ashburner *F >From: jr32@mole.bio.cam.ac.uk (John Roote) *F >Subject: 7-10-5 *F >Content-Length: 353 *F > *F >Michael, *F > *F >The synthetic lethal that was EMS-induced on TE35B and was originally *F >called 7-10-5 I have renamed l(2)TE35B-1. *F >1) Happy? *F >2) Can you think of a better name? *F >3) Will you tell Flybase? *F > *F >J *F > *F > *F >Department of Genetics *F >University of Cambridge *F >Downing Street *F >Cambridge *F >CB2 3EH *F >UK *F > *F >Tel: 44 223 333982 *F >Fax: 44 223 333992 *F >email: j.roote@gen.cam.ac.uk *F > *F > *F > *F > *F > *F >----- End Included Message ----- *F > *F >1. no *F >2. l(2)35Ab *F >3. yes *F > *F > *F >I think this is better. Make a note to change MS when (!if) it *F >is accepted. *F > *F >*a l(2)35Ab *F >*b 2- *F >*c 35A3--35B1 *F >*c Included in Df(2L)fn2 *F >*c not included in Df(2L)fn3 *F >*x J. Roote, Personal communication to FlyBase, 16 November 1994 *F >*A l(2)35Ab[1] *F >*o ethyl methanesulfonate *F >*O Tp(1;2)TE35B *F ># *F > *F >If this is OK send me a note, including this message. *F > *F >Michael *F This is fine. *F J # *U FBrf0075331 *a Wohlwill *b A. *t 1994 *T personal communication to FlyBase *u *F From kaufman@sunflower.bio.indiana.edu Tue Dec 20 21:07:58 1994 *F Date: Tue, 20 Dec 1994 16:31:23 -0500 *F To: ma11 *F From: kaufman@sunflower.bio.indiana.edu (T. Kaufman) *F Subject: Re: help!!!!!!! *F Content-Length: 2003 *F >FLybase directors: I noticed that T(2:3)W and T(3:4)antpW were listed *F >as entries for which more information was needed. Brenda Leicht and I *F >seperately isolated some mutants that looked like ANTP, so we gave them *F >to Thom Kaufmann who then characterized them further. I was actually *F >suprised to find that had isolated the mutant described as T(3;4)AntpW, *F >I had thought this was the one that Brenda had identified. At any rate *F >Thom Kaufmann should be able to clear up any questions you might have *F >about this stock. Arthur *F >------------------------------------------------ *F >*a T(2;3)W *F >*B 33E;66C *F >*C T *F >*o X ray *F >*w Wohlwill *F >*Q Isolated simultaneously with T(3;4)Antp[W] *F ># *F >*a T(3;4)Antp[W] *F >*z FBab0010378 *F >*H Last updated 05 Dec 94 *F >*B 84B1-2;102F *F >*C Translocation *F >*w Wohlwill *F >*o X ray *F >*A FBal0000618 == Antp[W] *F >*Q Isolated simultaneously with T(2;3)W *F ># *F Michael: He only gave me one stock with an Antp phenotype. Actually it is *F rather more like Ctx than a generic Antp gain of function. The cytology *F recorded in my notebook says there is a double translocation - the first is *F T(2L;3L) broken in 33E and 66C. The other is T(3R;4) broken in 84B1,2 and *F 102. The later is the most likely cause of the Antp mutant phenotype. I *F have never tried to separate the two translocations to see if the last *F statement is true but just kept the balanced stock which still produces a *F nice phenotype. I have no information on any other stock from Brenda. *F This mutant is listed in L&Z with Arthur as the isolator. Some of the stuff *F in that table was a core dump from my cytology notebook sorry about the *F confusion. *F *F >while you are trawling the grey cells any help on these orphons ? *F Will get to the next shortly. Km and I recall having discussed these at *F one point and getting together the relevant info. We are looking for that *F now. --tk *F Thom Kaufman kaufman@sunflower.bio.indiana.edu *F --- Biology Dept., Indiana University, Bloomington, IN 47405 --- *F 812-855-3033/Office--812-855-7674/Lab--812-855-2577/FAX # *U FBrf0075337 *a Mason *b J.M. *t 19?? *T personal communication to FlyBase *u *F Archived # *U FBrf0075338 *a Rawson *b R. *t 1992 *T personal communication to FlyBase *u *F Archived # *U FBrf0075340 *a Brower *b D. *t 1992 *T personal communication to FlyBase *u *F Archived # *U FBrf0075341 *a Degelmann *b A. *t 1992 *T personal communication to FlyBase *u *F Archived # *U FBrf0075342 *a Lewis *b E. *t 1992 *T personal communication to FlyBase *u *F Archived # *U FBrf0075371 *a Green *b P. *t 1995 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Dec 20 10:48:54 1994 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 20 Dec 94 10:48:44 GMT *F To: green@biovx1.biology.ucla.edu *F Subject: Re: abstract from 33rd ann Dros Res conference, 1992 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1731 *F Dear Dr Green, *F I apologise for getting back to you at such a later date but curation *F of the 33rd ADRC abstract book has brought me back to the original *F abstract I queried you about and I have remembered a question I should *F have asked the first time round. *F Our policy, now, is never to curate the posters from which an abstract *F is based. This is because the information from the poster is never *F published as the poster itself, but as a subsequent publication which *F will get curated by FlyBase and therefore be entered into the *F database. Before this descision was made Prof. M. Ashburner made a *F practice of recording gene names from each poster to which the abstract *F could be referenced. *F Consequently we now have your reference: *F \*x FBrf0055320 == Green et al., 1992, A. Conf. Dros. Res. 33: 48 *F against a series of genes and alleles and I have no way of knowing by *F which means they arrived there. These genes and alleles have very *F little information except what was possibly stated on your poster. I *F realise this was a while ago but have you published anymore information *F about the following genes so they can be correctly referenced and be *F more fully curated? *F \*a fas *F \*A fas[G5] *F \*x FBrf0055320 == Green et al., 1992, A. Conf. Dros. Res. 33: 48 *F \*C T(2;3)50B7-50B9;100D-100E = T(2;3)fas[G5-29] *F \*a old *F \*e optic lobe defective *F \*b 2-[56] *F \*c 42E3--43C3 *F \*x FBrf0055320 == Green et al., 1992, A. Conf. Dros. Res. 33: 48 *F \*a shg *F \*A shg[G137] *F \*x FBrf0055320 == Green et al., 1992, A. Conf. Dros. Res. 33: 48 *F \*C In(2R)42B1-42B3;58A3-58A4 = In(2R)shg[G137] *F Any help would be greatly appreciated, *F Happy holidays *F Eleanor Whitfield, *F on behalf of FlyBase. *F Your information concerning the enhancer trap was marvellous, *F Thanks again. *F From green@biovx2.biology.ucla.edu Wed Jan 4 00:15:54 1995 *F Date: Tue, 3 Jan 1995 16:04:52 -0800 *F From: green@biovx2.biology.ucla.edu *F To: eleanor@gen.cam.ac.uk *F Subject: Re: abstract from 33rd ann Dros Res conference, 1992 *F X-Vms-To: SMTP%'eleanor@gen.cam.ac.uk' *F Content-Length: 1510 *F HAPPY NEW YEAR, ELEANOR! *F I was celebrating my recent defense, and I have been on vacation, so I just *F got your e-mail. Sorry about the delay. *F Here is the scoop on the genes you asked me about. *F optic lobe defective: we were calling the P-element tagged sine oculis locus *F optic lobe defective, because we hadn't realized that the locus was actually *F sine oculis, yet. In a later poster (34th, I think) the locus had been renamed *F and called absent minded (ami)(this reflected a collaboration which had been *F started and we took over the name the other group had for it). We changed *F the locus name to sine oculis as soon as we figured it out. *F fas: faint sausage. This gene is being cloned and sequenced in our lab and *F a paper will be submitted probably by August of 1995. Subsequent queries should *F be made to arne lekven: lekven@biovx1.biology.ucla.edu since he is the one *F who is cloning it. I believe he has published one other abstract, but no other *F papers utilizing this mutant. *F shg: shotgun. This gene is also being cloned and sequenced in our lab. There *F has been one further Dros Conf poster, by Tepass, I think. Anyway, you should *F make inquiries to Ulli Tepass at tepass@biovx1.biology.ucla.edu since he is *F the person who will eventually clone this gene. *F Both fas and shg were isolated by Nusslein-Volhard,Weischaus, etc in THE BIG *F F2 screen. *F I am sorry I could not be more helpful on these two loci. Ulli and Arne will *F probably be better able to fill you in. *F Have a nice day, *F Patti # *U FBrf0075381 *a Baker *b B.S. *t 1994 *T personal communication to FlyBase *u *F From bbaker@cmgm.stanford.edu Wed Apr 13 19:38:28 1994 *F Date: Wed, 13 Apr 1994 11:42:17 PST *F From: BAKER LAB *F Subject: Re: T(1;3;4)dsx[M+R41] *F To: eleanor *F I have gotten out the original drawing of the above rearrangement. I can add *F a bit more, but probably not everything. *F a. 3R is normal from the base to 100A where it is capped with the tip of the X *F (1-3) *F b. the tip of 3L (61-64 sticks out of the chromocenter and it is unclear what *F it is attached to. The cell has a split chromocenter and the base of the 4 can *F be seen in the part of the chromocenter into which the 3L tip is inserted. It *F could be the 3L tip caps the 4th. *F c. The X is normal from the base to salivary region 3 where a piece of 3L is *F attached and this is inturn capped with most of the 4th. *F d. Which part of 3L is on the X is not completely clear from the drawing. I *F have 3L normal and paired with its homologue from the base to 70/71 where the *F rearranged 3L is capped with the tip of 3R (100F-A). There is then an unpaired *F segment of the normal 3L and it then becomes paired again as it synapses with *F the piece of 3L that is attached to the X. Putting this together I would *F infer that it is the region 70/71-64 of 3L that is attached to the X. However *F the label says 64-67. From looking at the drawing it looks like it may be a *F piece about 64-67 in size from the X/3L/4tip piece that was paired with the *F normal 3rd and there is then an asynnapsed piece that somehow didn't get *F accounted for that could correspond to 67-71. *F So my best guess is: *F 4th/64-70 or 71/3-20cc *F 100F-A/70 or 71-80cc *F 61-64/cc (4 base by inferrence) *F 1-3/100A-81cc # *U FBrf0075382 *a Baker *b B.S. *t 1994 *T personal communication to FlyBase *u *F From bbaker@cmgm.stanford.edu Tue Apr 26 05:06:02 1994 *F Date: Mon, 25 Apr 1994 21:00:02 PDT *F From: BAKER LAB *F Subject: Re: T(1;3;4)dsx[M+R35] *F To: eleanor *F Content-Length: 1112 *F Dear Eleanor, *F Sorry for the delay in this reply but I have been out of town for the past 5 *F days. Re: T(1;3;4)dsx[M+R35]. Not sure I can be as much help on this one, but *F here is what I can say from the original drawing and notes. *F 1. The distal part of 3L (61-66C) is stuck onto something heterochromatic as *F it sticks out of the chromocenter. *F 2. This distal part of 3R (98-100) also appears to be stuck onto something *F heterochromatic as it also sticks out of the chromocenter. *F 3. 3R from the base to 98 is normal and stuck onto 98 is 66C and the rest of *F 3L (to 80C) which is attached to something; perhaps the 4th chromosome. *F 4. the obvious problem here is that while one can determine the euchromatic *F breaks and the new euchromatic-euchromatic associations if there are multiple *F heterochromatic breaks (as this rearrangement appears to have) one cannot *F specify from salivaries what are the new euchromatic-heterochromatic *F associations. Sorry I can't give you more definitive information, but this is *F probably all that can be gotten from the type of observations that were made. *F Sincerely, Bruce Baker # *U FBrf0075385 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F Aubrey, *F Me again. My last message was premature. I've just found MA's original *F notes. The true order is neither of those 2 possibilities. *F He saw a 38|42 junction and a 35-38 deletion so the order is: *F 21--35F|38C--*--42B|38C--21 *F J # *U FBrf0075399 *a Orr-Weaver *b T.L. *t 1994 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Nov 24 15:20:40 1994 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 24 Nov 94 15:20:09 GMT *F To: weaver@wi.mit.edu *F Subject: Deficiencies in the 58B-58D region *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1850 *F Dear Dr. Orr-Weaver, *F I am a member of the FlyBase consortium at the Cambridge, England site, *F working with Prof. M. Ashburner. I am at present curating abstracts *F from the 31st A. Dros. Res. Conf. Within our aberrations file we have *F four deficiencies, the only reference available for these is an *F abstract of yours: *F \*a Df(2R)X58-1 *F \*x FBrf0064306 == Kerrebrock and Orr-Weaver, 1990, A. Conf. Dros. Res. 31: 45 *F \*a Df(2R)X58-3 *F \*x FBrf0064306 == Kerrebrock and Orr-Weaver, 1990, A. Conf. Dros. Res. 31: 45 *F \*B 58C1-58C2;58E1-58E2 *F \*a Df(2R)X58-5 *F \*x FBrf0064306 == Kerrebrock and Orr-Weaver, 1990, A. Conf. Dros. Res. 31: 45 *F \*B 58B1-58B2;59A1 *F \*a Df(2R)X58-7 *F \*x FBrf0064306 == Kerrebrock and Orr-Weaver, 1990, A. Conf. Dros. Res. 31: 45 *F \*B 58A1-58A2;58E4-58E10 *F star coding represents: *F \*a aberration symbol *F \*x reference *F \*B breakpoints *F When reading the abstract it is apparent these deficiencies are not *F named and the cytology is not given. Can I assume this level of detail *F was present on your poster? If this assumption is correct, then these *F aberrations are incorrectly referenced as we do not, as a policy, *F curate from posters. What I require is conformation that these *F aberrations do exist and any more information concerning them you *F have. All information you can supply will be entered into the database *F as a personal communication and the abstract reference shall be *F removed. *F Your help will be much appreciated, *F Eleanor Whitfield *F \-------------------------------------------------------------- *F Eleanor Whitfield. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email : eleanor@gen.cam.ac.uk *F Cambridge, CB2 3EH, Phone : 0223-333963 *F UK. FAX : 0223-333992 *F \-------------------------------------------------------------- *F From weaver@wi.mit.edu Mon Dec 5 19:16:30 1994 *F To: eleanor@gen.cam.ac.uk (Eleanor Whitfield Genetics) *F From: weaver@wi.mit.edu (Terry Orr-Weaver) *F Organization: Whitehead Institute for Biomedical Research *F Return-Receipt-To: weaver@wi.mit.edu *F Date: Mon, 05 Dec 1994 14:11:43 EST *F Subject: RE: Deficiencies in the 58B-58D region *F Content-Length: 1152 *F Dear Dr. Whitfield: *F When she was in my lab Anne Kerrebrock generated a collection of *F deficiencies in the 58 region, but we have never published these. I do not *F know where you obtained the information regarding the deficiencies, *F possibly off a poster at a Fly meeting. I have listed below the set of *F deficiencies we have, and you may cite them as a personal communication. *F We make these stocks freely available to anyone who requests them, and I *F should probably send them to Bloomington. The names are the isolation *F numbers from Anne's screen; I realize they may not conform to Flybase *F nomenclature, but I had planned to use these names when we publish them in *F a paper that is now in preparation. *F Df(2R)X58-1 58D6-8; F3-5 *F Df(2R)X58-2 58C7-D2; F3-5 *F Df(2R)X58-3 58C3-7; D6-8 *F Df(2R)X58-4 58C3-7; E *F Df(2R)X58-5 58A3-B2; 59A *F Df(2R)X58-6 58A3-B2; E3-10 *F Df(2R)X58-7 58A1,2; E3-10 *F Df(2R)X58-8 58A1,2; F3-5 *F Df(2R)X58-9 58A1-B2; 58F *F Df(2R)X58-10 not determined *F Df(2R)X58-11 58A3,4; E3-7 *F Df(2R)X58-12 58D1,2; 59A *F In(@LR)dpp[t24L]dpp[d75R] 58B; 58D *F Please let me know if you require additional information. *F Sincerely, *F Terry *F Orr-Weaver # *U FBrf0075401 *a Green *b P. *t 1994 *T personal communication to FlyBase *u *F Dear Dr. Green, *F I am a member of the FlyBase consortium at the Cambridge, England site, *F working with Prof. M Ashburner. At present I am curating abstracts *F from the 33rd Annual Drosophila Research Conference, 1992. *F You have an abstract on page 48: Genetic study of the embryonic *F development of the optic lobe. Within the abstract you do not name the *F enhancer trap line under study. Do you know designation of the line, *F do you know the gene into which the enhancer trap has inserted, and do *F you know the lethal alleles recovered from reversion of the line? *F Any information you can give us concerning the enhancer trap itself, *F its insertion point and consequent excision would greatly help FlyBase *F to generate a more comprehensive report of the use of lacZ enhancer *F trap lines. The data you can give me will be referenced in FlyBase as *F a personal communication from yourself, as the information you will be *F sending is not strictly present within the text of the abstract. *F Many thanks, *F Eleanor Whitfield. *F From green@biovx1.biology.ucla.edu Fri Dec 9 08:48:17 1994 *F Date: Thu, 8 Dec 1994 13:54:45 -0800 *F From: green@biovx1.biology.ucla.edu *F To: eleanor@gen.cam.ac.uk *F Subject: abstract from 33rd ann Dros Res conference, 1992 *F X-Vms-To: SMTP%'eleanor@gen.cam.ac.uk' *F Content-Length: 1121 *F Dear Dr. Whitfield, *F I am replying to your recent letter re: the use of the enhancer trap line *F in the abstract, genetic study of the embryonic development of the optic lobe. *F The enhancer trap line used for that study was called A6-2-45. It came from the *F enhancer screen reported by Bier, et. al, 1989 (Genes Dev. 3:1273-1287.) *F We have also used a similar enhancer, generated in a screen at UCLA, called *F BE85. *F Both of these enhancer line have the P-element inserted at 43C on the second *F chromosome. Both are inserted within 1 kb of the transcriptional start site *F of the sine oculis gene. We characterized this gene in the paper, Cheyette, *F et. al, 1994 (Neuron, 12: 977-996). We published the data from the abstract *F you are asking about in Green, 1993 (Cell Tiss. Res. 273:583-598). *F We made excisions of the P-element in BE85 and got 2 lethal excisions removing *F the start site of the sine oculis gene. We also got a few hypomorphic alleles *F that affect the size of the adult eye. *F If you have any further questions, you can send me an e-mail, if that would *F be easier. *F I hope this is helpful. *F Patti Green *F From eleanor@gen.cam.ac.uk Fri Dec 9 09:15:32 1994 *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 9 Dec 94 09:15:22 GMT *F To: green@biovx1.biology.ucla.edu *F Subject: Re: abstract from 33rd ann Dros Res conference, 1992 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 469 *F Dear Dr. Green, *F Thank you for your very comprehensive answer. I shall curate a personal *F communication embracing all the relevant information from your message. *F Giving me the further references to look at will be very helpful. *F I apologise for having to transmit this request via Glenn McAlpine but *F the fax machine here refuses to talk to your fax machine, but they both *F receive and send faxes elsewhere successfully! Very strange! *F Many thanks, *F Eleanor Whitfield. # *U FBrf0075402 *a Siegel *b V. *t 1994 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed Dec 7 11:01:44 1994 *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 7 Dec 94 11:01:38 GMT *F To: siegel@wi.mit.edu *F Subject: 33rd ADRC abstract *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1559 *F Dear Dr. Siegel, *F I am member of the FlyBase consortium working with Prof. M. Ashburner *F at the Cambridge, England, site. At present I am curating abstracts *F from the 33rd Annual Drosophila Research Conference, 1992. On page 25 you *F have an abstract: Analysis of a locus affecting both anterior-posterior *F and dorsal-ventral axis formation during oogenesis. *F At present within the database we have this abstract referenced under *F bwk; bullwinkle. This seems unlikely to me as this gene is on a *F different chromosome from the P-element insert the abstract discusses. *F To help unravel the apparent confusion could you please tell me what *F the locus under study is? What are the P-element insertion allele *F designations and the subsequent revertant allele designations? Is this *F data published elsewhere? *F Any information you can give us would greatly help FlyBase to generate *F a more comprehensive report of the gene under study. The data you can *F give me will be referenced in FlyBase as a personal communication from *F yourself, as the information you will be sending is not strictly *F present within the text of the abstract. *F Many thanks, *F Eleanor Whitfield. *F \-------------------------------------------------------------- *F Eleanor Whitfield. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email : eleanor@gen.cam.ac.uk *F Cambridge, CB2 3EH, Phone : 0223-333963 *F UK. FAX : 0223-333992 *F \-------------------------------------------------------------- *F From siegel@wi.mit.edu Thu Dec 8 20:31:15 1994 *F To: eleanor@gen.cam.ac.uk (Eleanor Whitfield Genetics) *F From: siegel@wi.mit.edu (Vivian Siegel) *F Organization: Whitehead Institute for Biomedical Research *F Date: Thu, 08 Dec 1994 15:26:04 EST *F Subject: RE: 33rd ADRC abstract *F Content-Length: 550 *F The locus is pipsqueak (reference Development Dec 1993). All the alleles and *F the phenotypic characterization is in there. There should be another paper, *F too, fairly soon, from Ursula Weber and Marek Mlodzik (and me) about *F phenotypes in the eye and the molecular characterization of the gene. It *F was also known as zeppelin (in the Spradling review). The original allele *F is psqP1, and the revertants are known as psqx1-30 and psqx1-36. But all *F this information and more is in the Development paper. Feel free to write *F if you have further *F questions. *F From eleanor@gen.cam.ac.uk Fri Dec 9 08:31:17 1994 *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 9 Dec 94 08:31:11 GMT *F To: siegel@wi.mit.edu *F Subject: RE: 33rd ADRC abstract *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 314 *F Dear Dr. Siegel, *F Thank you very much for your prompt response. I shall curate a *F personal communication which shall include psq, all alleles and *F minimal phenotypic information from Development 119: 1187. This paper *F shall be curated in the very near future by my fellow curator. *F Many thanks, *F Eleanor Whitfield. # *U FBrf0075403 *a Matthews *b B. *t 1994 *T personal communication to FlyBase *u *F any of that in Flybase but hopefully it will be published soon with the new results. *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 7 Dec 94 11:39:10 GMT *F To: bmatthew@morgan.harvard.edu *F Subject: cdi insertion, 33rd ADRC *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 928 *F Hello Bev, *F I'm curating the 33rd ADRC at the moment and you have an abstract on *F page 52 about an enhancer trap lines insertion into cdi. Have you *F named the enhancer trap and/or the insertion alleles? Have you *F published this data anywhere else? *F As I am sure you know we have very little about cdi in the data base at *F the moment, this abstract is the only reference, so all you can tell me *F will be useful. And, I'm sure I don't really need to tell you this *F but, the data you give me will be entered in the database as a personal *F communication from yourself as the info is not strictly within the text *F of the abstract. *F Ele *F From bmatthew@morgan.harvard.edu Fri Dec 9 16:40:20 1994 *F Date: Fri, 9 Dec 94 11:41:57 EST *F From: bmatthew@morgan.harvard.edu (Beverley Matthews) *F To: eleanor@gen.cam.ac.uk *F Subject: Re: cdi insertion, 33rd ADRC *F Cc: bmatthew@morgan.harvard.edu *F Content-Length: 3826 *F Hi Ele, *F *F > I'm curating the 33rd ADRC at the moment and you have an abstract on *F > page 52 about an enhancer trap lines insertion into cdi. Have you *F > named the enhancer trap and/or the insertion alleles? *F Three different enhancer trap lines were isolated and two (87 and 242), were mapped to the first exon (5' untranslated region) and one (BA01), to the first intron of cdi. The 242 enhancer trap line and probably 87 as well have already been cited in other papers. I'm not sure about BA01. The insertions were all viable and weren't named as alleles. About 20 lethals were isolated by excising the P element and their names are not at all informative and they haven't been published. I guess they'll be renamed 1-20 or whatever. *F > Have you *F > published this data anywhere else? *F No I haven't. It's been sitting on my CV as 'in preparation' for years. The lab that I did the work in was trying to carry on (very slowly) and find a phenotype (other than lethality) for my mutants. As it turns out, I just found out that the gene was independently found by another lab as a target gene for Ubx. We sent them my mutants and they found gut defects in embryos. It's too early to put any of that in Flybase but hopefully it will be published soon with the new results. *F By the way the genes file says the gene name is central divider while in fact the name we gave it is center divider. Do I need to write a formal letter to Flybase to correct that? *F > As I am sure you know we have very little about cdi in the data base at *F > the moment, this abstract is the only reference, so all you can tell me *F > will be useful. And, I'm sure I don't really need to tell you this *F > but, the data you give me will be entered in the database as a personal *F > communication from yourself as the info is not strictly within the text *F > of the abstract. *F I don't really have that much to add to the record except perhaps the lethal alleles which were caused by P element excision and were mapped as small deletions (mostly ~1-3 kb) which take out the transcription start. 10 of them will appear in the published figure. If you want to put any of that in I can give you more specific information. I'm having a hard time finding it at the moment. *F Bev *F From eleanor@gen.cam.ac.uk Mon Dec 12 08:42:27 1994 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 12 Dec 94 08:42:21 GMT *F To: bmatthew@morgan.harvard.edu *F Subject: Re: cdi insertion, 33rd ADRC *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 705 *F Dear Bev, *F Thank you for your swift reply. Good to hear all is well with you. I *F have to admit that I queried your abstract for more details as I knew *F you would reply. I hope I didn't cause too much of a distraction. *F The information you gave was exactly what I needed. The enhancer trap *F lines have been named and some information given that I can put in *F to beef up the proforma. Regarding the correction to the gene name I *F have incorporated that into the personal communication so when this is *F integrated into the database the gene name should change. | have used *F the: *F \!c G2a. Gene name to use in database :center divider *F format devised by Wayne. *F Happy curating! *F Eleanor. # *U FBrf0075410 *a Oliver *b B. *t 19?? *T personal communication to FlyBase *u *F Archived # *U FBrf0075411 *a Gelbart *b W.M. *t 1994 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Fri Feb 18 15:22:12 1994 *F Date: Fri, 18 Feb 94 10:17:13 EST *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: rd120@gen.cam.ac.uk *F Subject: Re: T(1;2;3)878.3 *F Cc: flybase@morgan.harvard.edu *F Content-Length: 2266 *F Status: RO *F X-Lines: 60 *F The dreaded T(1;2;3)878.3 is in FlyBase terms: *F TS3C(1;3)sc[260-15] + TS2C(2;3)H38 + TS2C(2;3)DTD61 *F T(2;3)DTD61 = T(2;3) 40D-F ; 100A (formerly T(2;3)518.18) *F T(2;3)H38 = T(2;3) 41A ; 71C1-2 (formerly T(2;3)514.2-2-192) *F (Originally, I thought the cytology was 40B-D, but on *F reexamination, I corrected the proximal 2 breakpoint to 41A. *F I don't know if the original cytology was posted anywhere, *F but I'm telling you in case you run into it and are *F perplexed as to which is correct.) *F T(1;3)sc[260-15] = T(1;3) 1B4-5 ; 71C-D *F (Hmmm? My notes say 71D-E. I can't find the original data *F that explains this update; it may be through my own *F examination, or it might be someone else -- perhaps Michael. *F You may want to check with Peter Cherbas on the current *F status of the cytology. I made this construct to help with *F EIP28/29 region genetics for him.) *F The rearranged chromosomes are ordered as follows: *F 1t--1B4-5 | 71C-D (or 71D-E)--100t *F 61t--71C1-2 | 41A -- 21t *F 60t--40D-F | 100A--100t *F This is deleted for the interval between the 71C1-2 breakpoint of H38 *F and the 71C-whatever breakpoint of T(1;3)sc[260-15]. Further, we know *F from molecular mapping of breakpoints that the H38 breakpoint was *F distal to the sc[260-15] breakpoint. *F Bill # *U FBrf0075419 *a Crowley *b T. *t 1994 *T personal communication to FlyBase *u *F Archived # *U FBrf0075420 *a Lyttle *b T.W. *t 1994 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Nov 21 13:08:50 1994 *F From: Rachel Drysdale (Genetics) *F Date: Mon, 21 Nov 94 13:08:19 GMT *F To: lyttle@uhccvx.uhcc.hawaii.edu *F Subject: Dp(2;Y)cb25-9 and Dp(2;Y)cb25-11 *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 1030 *F Hi Terry, *F I am working on your 1989 Genetics Rsp paper *F The effect of novel chromosome position and variable dose on the *F genetic behavior of Rsp....... *F Genetics 121:751-763 *F and have a question about the cytology of the two 'carve-down' *F duplications that were examined cytologically, Dp(2;Y)cb25-9 and *F Dp(2;Y)cb25-11. *F You say (Fig 2) that one of the breaks made in generating these falls *F in h46-41A1. My question is about the second break. Do you know *F whether there is any distal 2R left on the duplication? Or is the *F second break so close to the telomere of 2R that no 2R euchromatin *F remains? *F many thanks for your help, *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 0223-333963 *F UK. FAX: 0223-333992 *F \-------------------------------------------------------------- *F From tlyttle@uhunix.uhcc.hawaii.edu Mon Nov 21 20:16:57 1994 *F Date: Mon, 21 Nov 1994 10:15:21 -1000 *F From: Terrence Lyttle *F To: rd120 *F Cc: *F Subject: Re: Dp(2;Y)cb25-9 and Dp(2;Y)cb25-11 *F Content-Length: 542 *F Rachel, *F Both -9 and -11 appear to be broken in the neighborhood of 60D. It is *F hard to be precise about these, because of heterochromatinization and other *F difficulties in mapping bands buried next to the chromocenter. An extra *F problem arises with -9, which actually appears to have one break in 42A. If *F you notice, -9 and -11 on Figure 2 of the paper actually are shown to have *F uncertain proximal breakpoints, beyond the h bands. I do think -11 has a *F more proximal breakpoint than -9, however. Does this help? *F Cheers, *F Terry *F \--------------------------------------------------------------- *F Hi Terry, *F thank you for your prompt response! Yes, it helps a lot and improves *F what we can store about these duplications a great deal. *F So to confirm,it sound to me like you would like to say that the two *F carve-down deletion breaks are: *F 42A and 60D, for Dp(2;Y)cb25-9 *F and *F 41A1-41F (i.e. beyond the h bands but proximal to the cb25-9 break) and *F 60D, for Dp(2;Y)cb25-11 *F We all know how difficult it is to do cytology close to heterochromatin, *F and it presents no problem to us to have a wide range in the breakpoint *F estimates. *F best wishes, *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 0223-333963 *F UK. FAX: 0223-333992 *F \-------------------------------------------------------------- *F From tlyttle@uhunix.uhcc.hawaii.edu Tue Nov 22 21:21:56 1994 *F Date: Tue, 22 Nov 1994 10:50:20 -1000 *F From: Terrence Lyttle *F To: rd120 *F Subject: Re: Dp(2;Y)cb25-9 and Dp(2;Y)cb25-11 *F Content-Length: 42 *F Rachel, *F You have it right. *F Terry # *U FBrf0075421 *a McKeown *b M. *t 1995 *T personal communication to FlyBase *u *F >From mike_mckeown@qm.salk.edu Fri Dec 23 16:47:46 1994 *F Date: 23 Dec 1994 08:49:09 -0800 *F From: 'Mike McKeown' *F Subject: Re: FlyBase needs help with *F To: 'ma11' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 1342 *F RE>>FlyBase needs help with some... 12/23/94 *F I didn't do the cytology, so I can't speak to its correctness. It is likely *F that either John Belote or someone in David Ish's lab did the listed cytology. *F John and I Iooked at hundreds of squashes together, so I trust his judgment *F as to cytology. Your listing matches Dan Lindsley's, so I suggest staying *F with it unless there is some (unknown to me) good reason to suspect its *F correctness. *F An additional correction not asked for in your letter. The lethal P insertion *F AS416, a ry+ chorion gene insertion from Spradling's lab, maps to 92E not to *F 92F. I make it about 92E3-4 based on in situs with a lambda phage containing *F the fragment interupted by AS416. This was originally noted by Ann Bang, then *F in Jim Posakony's lab at UCSD, as part of her studies of H. We have varified *F this mapping by complementation testing against Df(3R)oF4 (which complements *F AS416) and Df(3R)oB16 (which uncovers AS416). *F BTW: Happy Holidays to all at Flybase in the States and in Europe. You *F provide and invaluable service to all of us. I'm glad to have been able to *F help keep things up to date. *F Mike McKeown *F \-------------------------------------- *F Date: 12/23/94 4:04 AM *F To: Mike McKeown *F From: ma11 *F Dear Mike *F Thanks for your reply. Is the cytology we give correct ? *F [refers to Df(3L)h-i22] *F =========================== *F From mike_mckeown@qm.salk.edu Tue Jan 3 16:13:32 1995 *F Date: 3 Jan 1995 08:08:18 -0800 *F From: 'Mike McKeown' *F Subject: Re: helping flybase *F To: 'rd120' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 1177 *F RE>helping flybase 1/3/95 *F Rachel- *F AS416 does not interrupt H. It is in fact substantially proximal to H. AS416 *F is a lethal and revertable insert, but it affects a different gene from H. *F Ann was merely looking for potential starting points for walking to H and was *F checking transposons reputed to be in 92F when she found AS416 in 92E. *F Mike *F \-------------------------------------- *F Date: 1/3/95 4:04 AM *F To: Mike McKeown *F From: rd120 *F Dear Mike, *F I am curating this portion of your message to Michael: *F >An additional correction not asked for in your letter. The lethal P *F insertion *F >AS416, a ry+ chorion gene insertion from Spradling's lab, maps to 92E not to *F >92F. I make it about 92E3-4 based on in situs with a lambda phage containing *F >the fragment interupted by AS416. This was originally noted by Ann Bang, *F then *F >in Jim Posakony's lab at UCSD, as part of her studies of H. We have varified *F >this mapping by complementation testing against Df(3R)oF4 (which complements *F >AS416) and Df(3R)oB16 (which uncovers AS416). *F The implication is that the insert causes a lethal allele of H. Is this so? *F Happy New Year *F Rachel # *U FBrf0075422 *a Posakony *b J.W. *t 1994 *T personal communication to FlyBase *u *F Archived # *U FBrf0075505 *a Heilig *b J. *t 1995 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Joe Heilig, University of Colorado *F To: Bloomington Drosophila Stock Center *F Subject: phenotype of Adv[1] *F Dated: 27 Jan 1995 *F *F Background: This is additional information Joe Heilig got from Todd Laverty (Rubin lab) about the phenotype of Adv[1], an unpublished dominant mutation *F included in a stock contributed to the Bloomington collection by the Rubin lab. *F *F Information communicated: *F Adv[1] results in a sort of spur from L1 that runs further than L1 does normally. The is apparent at the base of the wing. The additional vein-like things in the w[1118] P{ry[+t7.2]=hsFLP}1; Adv[1]/CyO *F stock segregate with CyO. # *U FBrf0075506 *a Posakony *b J.W. *t 1995 *T personal communication to FlyBase *u *F Date: Fri, 3 Feb 1995 11:27:05 +0000 *F From: Jim Posakony *F To: Cahir O'Kane *F Subject: Re: 62A *F Dear Cahir, *F Hilary Ellis, a former postdoc who generated several deficiencies in the *F emc region when she was in my lab, has deposited a number of them -- *F including Df(3L)14-8 -- into the Bloomington stock center. I understand *F that the stock center lists it as Df(3L)emc5. If for any reason you have *F trouble getting it from Bloomington (we don't carry it here anymore), you *F can still get it from Hilary, who's at Emory University and is listed in *F Flybase. In her stocklist, it's stock number H5. *F Good luck, and let me know if you need anything else. *F Jim # *U FBrf0077917 *a Jacq *b B. *t 1995 *T personal communication to FlyBase *u *F Date: Tue, 28 Feb 1995 22:57:35 +0000 (WET-DST) *F From: Amos Bairoch *F Subject: New Dros. seq in swiss-prot + various points *F To: ma11@phx.cam.ac.uk *F Message-Id: <01HNLLR0RNMA002IBY@cmu.unige.ch> *F X-Envelope-To: ma11@phx.cam.ac.uk *F X-Vms-To: ASHBURNER *F X-Vms-Cc: BAIROCH *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 6760 *F X-Lines: 170 *F Status: RO *F *F Dear Michael, *F *F I visited B. Jacq lab and they released to SWISS-PROT 'corto' *F (FBgn0010313). The protein sequence is release to SWISS-PROT, but not *F yet the nucleotide entry. However, there is a nucleotide sequence entry *F in EMBL which correspond to 'corto', it is D43795, an unpublished *F sequence from a Japanese group. That sequence is very very sick (there *F are 52 frameshift errors !!!) (B.Jacq group has studied very carefully *F the sequence, has made antibodies against various part of his translation *F and he is confident he has the right protein and that the Japanese group *F is wrong. In addition the Japanese group is completely in the wrong as *F to the function of the protein, their CCAAT binding is an artefact of the *F non-specific DNA-binding activity of that protein). He will be able to *F tell you all about this very interesting protein in Atlanta. *F *F Best regards *F *F Amos # *U FBrf0077918 *a Rubin *b G.M. *t 1995 *T personal communication to FlyBase *u *F From gerry_rubin@maillink.berkeley.edu Thu Mar 2 19:46:13 1995 *F Date: 2 Mar 1995 11:36:14 -0800 *F From: 'Gerry Rubin' *F Subject: Re: abs in yr files *F To: 'ma11' *F Cc: ag24@gen.cam.ac.uk, ma11@gen.cam.ac.uk, spradling@mail1.ciwemb.edu, *F suzi@avery.lbl.gov *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 1800 *F *F Reply to: RE>abs in yr files *F *F Michael, *F *F ... *F *F Df(2R)E3363 *F This is a Df that was recovered as an X-ray induced allele in a screen for *F modifiers of activated Ras phenotype. Discovered by Marc Therrien. Cytology by *F Todd Laverty. Removes 47A-F. *F *F Df(2R)XE-916 *F This is a Df that was recovered as an X-ray induced allele in a screen for *F modifiers of activated Ras phenotype. Discovered by Felix Karim. Cytology by *F Todd Laverty. Removes 57F-58F. *F *F Regards, *F Gerry # *U FBrf0077919 *a Judd *b B.H. *t 1995 *T personal communication to FlyBase *u *F To: Friends of Flys *F From: Burke Judd *F Subject: Drosophila stocks and DNA clones. *F *F Some of the following are unique combinations of mutants that will be *F discarded when I leave the NIEHS, March 31, 1995. If you can use any *F of them please let me know. Phone (919)541-4690; FAX (919) 541-7593; *F e-mail judd_b@niehs.nih.gov. *F *F z[v77h] w[+] is from Oregon-R *F z[v77h] w[67c23] *F w[zm] *F sc z[1] w[zm] *F z[v77h] w[zm] *F y z[a] w[zm] *F y w[zl] *F z[1] w[zl] *F y z[a] w[zl] *F z[77h] w[zl] *F sc z[1] w[zvl] *F *F In(1)w[m4], y *F In(1)w[m4], y sn[3] *F In(1)w[m4], spl sn[3] *F In(1)w[m4], y z[1] *F In(1)w[m4], z[v77h] sn[3] *F In(1)w[m4h], z[v77h] *F In(1)rst[3] *F In(1)rst[3], y z[1] *F In(1)rst[3], z[v77h] *F *F Df(1)rst[2], y *F Df(1)rst[2], z[1] *F *F Df(1)Su(z)J93, y z[1] / FM7 The distal breakpoint of this deficiency is *F 35 to 60 kb proximal to the w locus and extends through rst and vt but does *F not include N. Deficiency acts as a dominant suppressor of z[1] apparently *F by acting on the w locus in cis. It also exhibits rst, vt, reduced viablity *F and female sterility. From deletion mapping against various rst and vt *F deficiencies, the suppressor of z[1] element is proximal to rst-vt. Deficiency *F occurs at very low frequency as an ectopic exchange product from females *F heterozygous for y[2] w[sp-2] and z[1] w[zm] or z[1] w[zl]. Several strains *F were recovered from both types of heterozygotes. Original recombinant *F chromosomes contained the w[zm] or w[zl] alleles. These have been replaced *F by crossingover with w[+] from Oregon-R or with w[65a25]. All versions of *F these derivatives and the parental chromosomes are available. CaSpeR plasmid *F clones of part of the region from +100 to +163 (white locus map). A *F transformed line carrying approx. 17.5kb extending from +122.5 to + 142 *F is available. Johng Lim, who did the transformation, also has a copy of *F this line. *F *F z[J91] This allele occurred spontaneously in z[1] w[65a25] spl sn[3]. *F It causes lemon-yellow eye-color in z[J91] w[+] males and z[J91] w[+]/z[+] w[+] *F females. It acts only in cis, however, thus most likely is acting on the *F w locus. *F *F z-w lethals: One allele of each of 13 lethals located in the region between *F the z and w loci will be deposited in the Mid-America Stock Center at Bowling *F Green. As many as three alleles for each locus will be kept here until the *F end of March. *F *F The deficiencies generated by ectopic recombination in the region flanking *F the w locus that are described in Montgomery et al., (1991) Genetics 129: *F 1085-1098, will be available until the end of March. *F *F echinus locus: We have cloned and sequenced genomic and cDNA that we believe *F to be the ec locus. However, a transformed line carrying the genomic sequences *F fails to rescue ec mutations, thus we have not yet published this. Fly strains *F and clones are available to anyone who is interested in a collaboration to *F complete this analysis. Contact Bibba Goode, Laboratory of Reproductive *F Toxicology, NIEHS, P.O. Box 12233, RTP, NC 27709 # *U FBrf0077920 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F >From JR32@phx.cam.ac.uk Tue Jan 4 16:32:37 1994 *F Date: Tue, 04 Jan 94 16:28:52 GMT *F From: JR32@phx.cam.ac.uk *F To: ag24@phx.cam.ac.uk, ma11@phx.cam.ac.uk *F Content-Length: 690 *F Status: RO *F X-Lines: 29 *F *F Aubrey, *F *F 2 alterations to the TE list that MA sent you a few weeks ago. *F The first is additional, the second is a correction. *F *F \*A Tp(1;2)TE35B-SR302 *F \*i TE35B-SR302 *F \*i TE146(Z:SR100:GZ3)SR302 *F \*b 2-50.0 *F \*c 35B1-35B2 *F \*p homozygous viable; mutant for noc *F \*O spontaneous derivative of Tp(1;2)TE35B(Z:SR100)GZ3 *F \*p Eye color on a w{-} background genotype: red, not suppressed by z{1} *F \*s FB[w{+} rst{+}] *F \*x *F \# *F \*A Tp(1;2)TE35B-SR401 *F \*i TE35B-SR401 *F \*i TE146(Z:SR100)SR401 *F \*b 2-50.0 *F \*c 35B1-35B2 *F \*p homozygous viable; mutant for noc *F \*O spontaneous derivative of Tp(1;2)TE35B(Z)SR100 *F \*p Eye color on a w{-} background genotype: red, not suppressed by z{1} *F \*s FB[w{+}, rst{+}]; 4 polytene bands *F \*x *F *F John # *U FBrf0077921 *a Okano *b H. *t 1991 *T personal communication to FlyBase *u *F Personal communication (#1863) from H. Okano to J. Merriam, 5 May 1991. *F Copy available from FlyBase by mail. # *U FBrf0077922 *a Jones *b R. *t 1992 *T personal communication to FlyBase *u *F Personal communication (#2829) from R. Jones to J. Merriam, 5 May 1992. *F Copy available from FlyBase by mail. # *U FBrf0077923 *a Roote *b J. *t 1994 *T personal communication to FlyBase *u *F J. Roote and M. Ashburner, personal communication to FlyBase 11 January 1994. *F From jr32@mole.bio.cam.ac.uk Fri Mar 10 13:42:29 1995 *F Date: Fri, 10 Mar 95 13:42:36 GMT *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: SW500 series *F Content-Length: 370 *F *F Michael, *F *F SW501 and 502 came from the stock of the synthetic lethal induced on TE35B *F called 7-10-5. The lethal is now known as l(2)35Ab. *F *F SW503 and 504 came from a modified version of the same stock. One of the *F Basc stocks had a very high level of NOF (or was it FB), so we tried to *F increase the NOF level of 7-10-5 by crossing to Basc and then recovering *F 7-10-5. *F *F John Roote # *U FBrf0077927 *a Cutforth *b T. *t 1994 *T personal communication to FlyBase *u *F From cutfort@rockvax.rockefeller.edu Wed Dec 14 19:37:38 1994 *F To: eleanor@gen.cam.ac.uk *F Subject: genbank entry *F Date: Wed, 14 Dec 1994 14:36:59 EST *F From: Tyler Cutforth *F Content-Length: 522 *F *F Dear Mrs. Whitfield, *F *F The Drosophila virilis cdc37 gene (accession L37055) corresponds to *F the Enhancer of sevenless gene E(sev)3B. The melanogaster gene *F was renamed cdc37 to reflect a structural and functional similarity *F with a budding yeast gene of the same name. *F *F The accession number for the homologous D. melanogaster gene is L32839. *F *F I hope this answers your questions. Please contact me if something *F remains unclear. *F *F Sincerely, *F *F Tyler Cutforth *F Box 248 *F Rockefeller University *F 1230 York Ave. *F New York, NY 10021 # *U FBrf0077928 *a Gelbart *b W.M. *t 1995.2.1 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Wed Feb 1 22:34:35 1995 *F *F Date: Wed, 1 Feb 95 17:35:31 EST *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: flybase-updates@morgan.harvard.edu *F Subject: M(2)LS1/M(2)23AB/dpp[Hin] *F Cc: gelbart@morgan.harvard.edu *F Content-Length: 4388 *F *F Michael, Aubrey, *F *F I'm not quite sure how to handle the case described in my *F email to Detlef Wolf (below). *F *F M(2)LS1 (= M(2)23AB in the Grey Book) was mapped to 22D-23B *F by Lindsley, Sandler et al. (1972). *F *F We drew the conclusion that what we originally called the 'Hin-d' *F locus (haplo-insufficiency near dpp) must be the entity that equates *F with M(2)LS1 (Spencer et al., 1982). In this paper, we also noted *F that Hin-d did NOT have a Minute phenotype. Further, in Irish and *F Gelbart (1987), we concluded that Hin-d was not an independent locus but *F rather was due to loss of one copy of the dpp gene, or more precisely *F due to complete knockout of one copy of its embryonic D/V patterning *F function defined by the so-called dpp[Hin] region. *F *F Neither Dan nor Larry had any notes that provided any information other *F than what was in the 1972 segmental aneuploidy paper. *F *F I have no idea why Michael renamed M(2)LS1 to M(2)23AB, since 23AB *F is a different cytological position from L&S`s 22D-23B. Michael, can *F you help? *F *F It seems like the current M(2)LS1 entry needs to be revised. Here are *F two options. *F *F (1) {My preferred option, but I leave it to the keepers of the *F genes Holy Grail.} *F *F Make both M(2)LS1 and M(2)23AB synonyms for dpp, and add a text comment *F somewhere in the genes record to the effect: 'The haplo-insufficient Minute *F locus proposed to map near dpp (previously called M(2)LS1 or M(2)23AB, *F Lindsley et al., 1972) is now thought to be the haplo-lethal but not *F phenotypically Minute loss of dpp[Hin] function (Spencer et al., 1982; *F Irish and Gelbart, 1987; Gelbart, pers. comm.).' *F *F (2) Keep the M(2)LS1 gene listing in place, add the synonym M(2)23AB, *F add the reference Lindsley et al. (1972) and add the same text statement: *F 'The haplo-insufficient Minute locus proposed to map near dpp (previously *F called M(2)LS1 or M(2)23AB, Lindsley et al., 1972) is now thought to be *F the haplo-lethal but not phenotypically Minute loss of dpp[Hin] function *F (Spencer et al., 1982; Irish and Gelbart, 1987; Gelbart, pers. comm.).' *F *F In reality, I think either option will clarify the situation. *F *F Bill # *U FBrf0077930 *a Modolell *b J. *t 1995 *T personal communication to FlyBase *u *F From JMODOL@mvax.cbm.uam.es Wed Mar 29 20:09:27 1995 *F Date: Wed, 29 Mar 1995 21:05:40 +0200 (WET-DST) *F From: JMODOL@mvax.cbm.uam.es *F To: ag24@gen.cam.ac.uk *F Subject: RE: AS-C question *F Content-Length: 1357 *F *F Dear Aubrey: *F I have checked our records on T7 and T9 and have asked Sonsoles *F Campuzano who has a fantastic memory about data obtained long ago. As *F far as we know, nobody has done anything else with these transcripts. *F What we know is that T9 should be transcribed from somewhere else in the *F genome, since Sonsoles observed it in Northerns containing RNA from the *F sc2 = ase1 deletion, which removes its putative transcription unit. With *F respect to T7, we did see it occasionally in our Northerns, but it *F seemed to be removed by more stringent washing of the blots. *F Incidentally, in Figure 2 of the Alonso and Cabrera paper I do not know *F for sure which transcript corresponds to which blot since the size and *F developmental profile of the T8 transcript (1.6 kb, present only in *F embryos) is very different from what we found (2.8 kb, present in *F embryos and late larva and early pupa; Gonzalez et al., 1989, EMBO J. 8, *F 3553-35623). And our results have been verified by the sequencing of *F cDNAs, primer extension experiments ( which indicate a minimal size for *F the mRNA, without the poly(A) tail, of 2.26 kb) and hybridizations in *F situ (which demonstrated its presence in late larva and early pupa). *F This is all I can say about these transcripts. I hope it is of some help. *F *F You can cite my e-mail in the fly base. Take care. Best regards, *F Juan # *U FBrf0077931 *a Roote *b J. *t 1995.3.24 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Fri Mar 24 18:43:00 1995 *F Date: Fri, 24 Mar 95 18:43:39 GMT *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Michael Ashburner *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: Help FlyBase - Roote 2 *F Content-Length: 3051 *F \*a Df(2L)chif64 *F 35F;36D *F made by John Tower, ref Tower et al 1993 Genetics _133_:347-359 *F mutant for cact+/- -- beyond dac *F overlaps RA5 by ~25kb *F \*a Df(2L)II30 *F made by John Tower, by excision of cact225 P insertion - one break at *F insertion site *F mutant for 35Fb -- cact *F \*a Df(2L)III18 *F made by John Tower, by excision of cact225 P insertion - one break at *F insertion site *F mutant for cni -- cact *F \*a Df(2L)RN2 *F 35E1.2;36A4.5 *F made by Roth, radiation(?) induced revertant of cacE10 *F 35Fg -- dac *F \*a Df(2L)Scorv16 *F Dawson's contribution was that rv16 carries a fzy mutation. *F \*a LS(2)TE35B-6 *F \*x Gubb, unpubl. *F This is TE146-GV6, 35B1;41 *F \*a T(2;3)pk78t *F \*x Gubb, unpublished *F pk-78t was mentioned in Gubb and Garcia-Bellido 1982 J. Embryol. exp. Morph *F 68:37-57. You discovered later that it was a T(2;3) *F \*a Df(2L)b79a3 *F \*u J. Roote pers. comm. May 1993 states that this chromosome does not *F \*u carry a deletion. *F from Alexandrov. *F A viable black allele, carries an allele of 35Ea *F \*a Df(2L)Adhn-BR55 *F 34E3;35B10 *F ENU induced by Bill Lee *F 34Eb -- 35Cb *F John Roote # *U FBrf0077933 *a Kennison *b J.A. *t 1991.12 *T personal communication to FlyBase *u *F Letter from Jim Kennison to J. Roote, December 1991. *F *F Copy available from FlyBase. # *U FBrf0077934 *a Ising *b G. *t 1989.5.17 *T personal communication to FlyBase *u *F Letter from Gunnar Ising to Claire Henchcliffe 17 May 1989 *F *F Copy available from FlyBase. # *U FBrf0077935 *a Wustmann *b G. *t 1989.4.4 *T personal communication to FlyBase *u *F Letter from Gerold Wustmann to G. Ising, 4 April 1989 *F *F Copy available from FlyBase. # *U FBrf0077936 *a Gergen *b J.P. *t 1995.3.27 *T personal communication to FlyBase *u *F From JGERGEN@ccmail.sunysb.edu Mon Mar 27 16:26:11 1995 *F Date: Mon, 27 Mar 1995 10:22:18 -0500 (EST) *F From: John Peter Gergen *F Subject: Re: Help Flybase _ Gergen *F To: ma11@gen.cam.ac.uk *F X-Vms-To: in%'ma11@gen.cam.ac.uk' *F Mime-Version: 1.0 *F Content-Transfer-Encoding: 7BIT *F Content-Length: 3299 *F State University of New York at Stony Brook *F Stony Brook, NY 11794-5215 *F John Peter Gergen *F Biochem. & Cell Biology *F 516 632-9030 *F 27-Mar-1995 09:40am EST *F FROM: JGERGEN *F TO: ma11 ( _ma11@gen.cam.ac.uk ) *F Subject: RE: Help Flybase _ Gergen *F Michael, *F With respect to Brother/ Big-brother, you certainly are being efficient. We *F submitted the sequences because we were getting pestered by people working on *F the mammalian 'homologs' for info and we thought the fairest way to deal with *F the diverse competition was to put the data in the public domain. We hope to *F submit the paper on these genes within the next few weeks. As you noted, the *F names we used for data base submission are Brother and Big-brother. The Brother *F designation stands for Beta for Runt and OTHERs. The beta comes from the name *F of the mammalian protein, PEBP2/CBFbeta, you know where Runt comes from and the *F Others part indicates our speculation that these widely expressed proteins will *F do more than just interact with Runt (even if they don't the observation that *F the proteins will interact with the mammalian Runt domain proteins already *F justifies the name to a certain extent). We are currently using a capital *F letter to refer to the gene because the name derives from a previously *F identified protein. We don't have mutants yet (it is in interval 62A10 and not *F uncovered by any extant deficiencies) but work in this direction is in progress. *F We have noticed that there appears to be a Minute in this region and wonder what *F the chances might be that our widely expressed genes correspond to this Minute. *F If so, then what would happen to the gene names? *F Big-brother is related but definitely a distinct gene. The two genes *F are closely linked, being separated by about 8 kb. The Big brother transcript *F as well as the deduced protein sequence are slightly larger than for Brother. *F Hence the name. As far as gene symbols goes (and I hope that this is not *F stepping on any toes...) we would like to use Bro as the symbol for Brother. *F If a 4 letter symbol is now obligatory then it should probably be Brot or Broh. *F Neither is as appealing as the three letter designation, hence our preference. *F For Big-brother we currently use Bgb in the lab. If this must be altered to 4 *F letters then Bgbr would be appropriate. As I mentioned above, we have not yet *F submitted a manuscript so these symbols and names are not etched in concrete. *F If you have comments, or suggestions then please send them back and we will *F consider them seriously. *F all best wishes, *F Peter Gergen # *U FBrf0077937 *a Mann *b R. *t 1995.4.4 *T personal communication to FlyBase *u *F From mann@cuhhca.hhmi.columbia.edu Tue Apr 4 15:39:19 1995 *F Date: Tue, 4 Apr 95 10:39:44 -0400 *F From: mann@cuhhca.hhmi.columbia.edu (Richard Mann) *F To: m.ashburner@gen.cam.ac.uk *F Subject: flybase update *F Content-Length: 833 *F Dear Michael, *F We now have good evidence that one of the Ubx target genes listed in *F flybase (lips) corresponds to the previously described zygotic *F lethal, canoe. The reasons are 1) we have sequenced a strong *F canoe allele and found a stop codon near the beginning of the *F lips ORF and 2) in the most recent issue of Genes and Development, *F a Japanese group claims to have sequenced canoe. Unfortunately they *F are probably right: their sequence is nearly identical to our lips *F sequence. However, if you read their paper, I think you'll agree that *F their evidence for saying this is canoe is not very compelling. In any *F case, I think it is appropriate that the flybase entry is updated so *F that there isn't any confusion. We will hopefully be submitting our version *F of the canoe molecular biology for publication soon. *F Best regards, *F Richard # *U FBrf0077938 *a Fischbach *b K.F. *t 1995.4.5 *T personal communication to FlyBase *u *F From kff@sun2.ruf.uni-freiburg.de Wed Apr 5 09:42:13 1995 *F Date: Wed, 5 Apr 1995 10:40:08 +0200 *F To: m.ashburner@gen.cam.ac.uk *F From: kff@ruf.uni-freiburg.de (Karl-Friedrich Fischbach) *F Subject: irre mapping *F Content-Length: 914 *F Dear Michael, *F the mapping of irreA, irreB, and irreD has been published in abstract form: *F Boschert U., Ramos G.P., and Fischbach K.-F. (1989). Genes required for *F optic chiasm formation in Drosophila melanogaster. In: Dynamics and *F Plasticity in Neuronal Systems. A 42. Eds. Norbert Elsner and Wolf *F Singer.Thieme Verlag. Stuttgart. New York. *F The mapping data of the mud locus have not been published and we have no *F new infos on that. *F Best regards, *F Karl *F Dr. K.-F. Fischbach Tel.: 0761-203-2730 *F Institut fur Biologie III Fax: 0761-203-2745 *F Schanzlestr.1 kff@sun1.ruf.uni-freiburg.de *F 79104 Freiburg i. Brsg. # *U FBrf0077939 *a Scott *b M. *t 1995.4.5 *T personal communication to FlyBase *u *F From scott@cmgm.stanford.edu Wed Apr 5 16:40:56 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 5 Apr 1995 11:40:42 -0500 *F To: ma11 *F From: scott@cmgm.stanford.edu (Matthew Scott) *F Subject: Re: Help FlyBase - Scott *F Content-Length: 154 *F Michael-- *F That's correct: braille and expanded are the same and the mapping of braille, in Lindsley and Zimm to 60B-C was a mistake. *F Best, *F Matt # *U FBrf0077940 *a Adler *b P. *t 1995.3.28 *T personal communication to FlyBase *u *F From daemon Fri Mar 24 12:14:42 1995 *F Received: from ppsw3.cam.ac.uk (pp@mauve.csi.cam.ac.uk 131.111.8.38) by *F faraday.clas.Virginia.EDU (8.6.10/8.6.6) with ESMTP id MAA75285 for *F ; Fri, 24 Mar 1995 12:14:25 -0500 *F Received: from ster.gen.cam.ac.uk by mauve.csi.cam.ac.uk *F with SMTP-CAM (PP-7.1) as ppsw.cam.ac.uk; *F Fri, 24 Mar 1995 17:12:51 +0000 *F Received: by ster.gen.cam.ac.uk (4.1/MDTG-V1.1@gen.cam.ac.uk) id AA28715; *F Fri, 24 Mar 95 17:17:37 GMT *F From: ma11 *F Date: Fri, 24 Mar 95 17:17:37 GMT *F Message-Id: <9503241717.AA28715@ster.gen.cam.ac.uk> *F To: pna@faraday.clas.virginia.edu *F Subject: Help FlyBase - Adler *F Cc: ma11@gen.cam.ac.uk *F Status: OR *F Paul *F FlyBase has got itself confused (ie I am confused) about the *F relationships to three 'genes; Arp, Psc and Su(z)2. *F There are two ways of sorting this out. *F 1. MA goes to the library for two days. *F 2. MA asks a man or knows ! *F I enclose the information now in FlyBase on these genes. I would be *F _very_ grateful if you could review it for us and tell *F us what the real biology is ! *F Best *F Michael *F From pna@faraday.clas.virginia.edu Tue Mar 28 18:45:04 1995 *F Date: Tue, 28 Mar 1995 12:41:36 -0500 *F From: 'Paul N. Adler' *F X-Mailer: Mail User's Shell (7.2.3 5/22/91) *F To: ma11@gen.cam.ac.uk *F Subject: flybase *F Content-Length: 38542 *F Michael, *F Appended is a revised version of the flybase report you mailed *F me. At the end I have added a note on the relationship between *F Arp, Psc and Su(z)2. *F Michael: I have made some corrections to the above, but I am not sure that all is correct as some *F of it refers to work not done in my lab and I might have missed minor problems. In a number of *F places Su(z)2 mutations are designated as PscSu(z)2x. Let me summerize some of my *F conclusions. Psc is located proximal to Su(z)2. The genes are transcribed divergently and their 5' *F ends are about 15 kb apart (this could be an overestimate as the 5' end of Psc was never well *F mapped). The two genes encode related proteins. In(2L)Arp breaks the chromosome between *F Psc and Su(z)2, but does not cause a loss of function in either. The juxtaposition of DNA from *F 49B upstream of Su(z)2 in In(2L)Arp results in a gain of function mutation in Su(z)2. This *F causes the arista to tarsus transformation and the bristle abnormalities. The bristle loss *F phenotypes of vg62 and vgD have a similar genetic basis. Indeed, we showed that *F overexpression of a hs-Su(z)2 transgene at the appropriate developmental stage could mimic the *F bristle loss phenotypes of these mutations (Sharp, Martin and Adler, Dev. Biol. 161:379-392 *F (1994). The overexpression of Psc from a hs-Psc transgene will also cause similar bristle *F phenotypes, but the overexpression of Psc is not involved in the gain of function phenotypes of *F Arp, vg62 or vgD (indeed Psc is deleted in vg62 and vgD). The overexpression of Psc at *F white prepupae results in substantial lethality and this may explain why no Psc overexpression *F mutations have been isolated that are analogous to the Su(z)2 overexpression mutations. *F Best wishes, *F Paul # *U FBrf0077941 *a Roote *b J. *t 1995.3.27 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Mar 27 13:10:43 1995 *F Date: Mon, 27 Mar 95 12:11:09 GMT *F X-Sender: jr32@mole.bio.cam.ac.uk (Unverified) *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: Help FlyBase - Roote 2 *F Content-Length: 3752 *F >*a Df(2L)b84h14 *F Df(2L)34D2;34F5 *F Alexandrov July 1991 DIS 70:16-19 *F gamma-rays, 20Gy *F 34Da -- 34Fa *F >*a Df(2L)b85b1 *F Df(2L)34D2;34D8 *F Alexandrov DIS 70 *F gamma, 10Gy *F 34Db -- 34Dg *F >*a Df(2L)b85b2 *F not visible *F Alexandrov DIS 70 *F gamma, 20Gy *F b -- 34Dc *F >*a Df(2L)b85c1 *F Df(2L)34D5;34F1.2 *F Alexandrov DIS 70 *F neutrons, 10Gy *F Sos -- 34Fa *F >*a Df(2L)b85f1 *F Df(2L)34C4.5;34F5 *F Alexandrov DIS 70 *F gamma, 20Gy *F 34Db -- wb *F >*a Df(2L)b85f1A *F Df(2L)34D3;34F1.2 *F Alexandrov (no ref - genetically different from b85f1) *F gamma *F 34Db -- rk *F >*a Df(2L)b87e25 *F Df(2L)34C1;35C1.2 cytology by Alexandrov *F Alexandrov DIS 70 *F gamma, 10Gy *F 34Da -- vasa *F >*a Df(2L)b88b42 *F not visible Alexandrov *F Alex *F neutrons, 2.5Gy *F b -- 34Dc *F >*a Df(2L)b88c75 *F Df(2L)34C5-7;35E2-5 Alexandrov *F Alexandrov DIS 70 *F neutrons, 5Gy *F 34Da -- 35Dg *F >*a Df(2L)b88f32 *F Df(2L)34D2-4;34E2-4 Alexandrov *F Alexandrov DIS 70 *F neutrons, 10Gy + gamma, 10Gy *F 34Db -- 34Eb *F >*a Df(2L)b88f43 *F Df(2L)34D2-4;34F1.2 Alexandrov *F Alexandrov DIS 70 *F neutron + gamma, 15Gy *F 34Da -- 34Fa *F >*a Df(2L)b88g83 *F Df(2L)34D2-4;34F1.2 Alexandrov *F Alexandrov DIS 70 *F neutron + gamma, 30Gy *F 34Db -- 34Dg *F >*a Df(2L)b88g96 *F Df(2L)34D2-4;34F1.2 Alexandrov *F Alexandrov DIS 70 *F neutron + gamma, 15Gy *F 34Db -- 34Fa *F >*a Df(2L)el90 *F don't know cytology *F Pascal Heitzler *F X-rays *F base chromosome pr cosV1 cn bw *F 34Db -- Adh *F >*a Df(2L)b84a9 *F not visible *F Glynnis Johnson *F gamma-rays *F pr pk cn sp *F Sos -- 34Dg *F >*a Df(2L)b74c6 *F Df(2L)34D1.2;34E1.2 *F Alexandrov DIS 70 *F gamma, 40Gy *F 34Db -- 34Eb, also 35BbAM10 *F >*a Df(2L)b77c *F Df(2L)34D2-4;34F4-35A1.2 Alexandrov *F Alexandrov DIS 70 *F gamma, 40Gy *F approx b -- rk, pu+ - extinct *F >*a Df(2L)b78j *F Df(2L)34D3;35A4 *F Alexandrov DIS 70 *F gamma, 40Gy *F 34Db -- wb *F >*a Df(2L)b79b3 *F Df(2L)34D1.2;34E5.6 *F Alexandrov DIS 70 *F neutrons, 10Gy *F 34Db -- 34Fa *F >*a Df(2L)b79b4 *F Df(2L)34D2-4;34E6-F1.2 Alexandrov *F Alexandrov DIS 70 *F neutrons, 10Gy *F 34Db -- wb *F >*a Df(2L)b79b8 *F Df(2L)34D2-4;34F4-35A1.2 Alexandrov *F Alexandrov June 1986 DIS 63:21-22 *F neutrons *F approx b -- rk, pu+ - extinct *F >*a Df(2L)b80f1 *F Df(2L)34C3;34E3-6 *F gamma *F base chromosome Sco *F 34Da -- 34Eb *F >*a Df(2L)b80k *F Df(2L)34D3;35B10 *F Alexandrov DIS 70 *F gamma, 40Gy *F 34Da -- 35Bd *F >*a Df(2L)b80l *F Df(2L)34D6;34E1.2 *F Alexandrov DIS 70 *F neutrons, 12Gy *F 34Db -- 34Eb *F >*a Df(2L)b81f1A *F Df(2L)34D1.2;34F1.2 *F Alexandrov (no ref - genetically different from b81f1) *F gamma *F 34Db -- 34Fa *F >*a Df(2L)b81f2 *F Df(2L)34D2-4;34F1.2 Alexandrov *F Alexandrov DIS 70 *F neutrons, 8Gy *F 34Db --34F1.2 *F (but the copy of 81f2 that we rec'd was 34D1.2;35B10, we called it 81f2A) *F >*a Df(2L)b81l42 *F Df(2L)34D3;34D8 *F Alexandrov DIS 70 *F gamma, 10Gy *F 34Db -- 34Dg *F >*a Df(2L)b82a3 *F don't know cytology *F Struhl *F HD (pi 2) *F b --34Dc - extinct *F >*a Df(2L)b83l1 *F Df(2L)34D4;34E1.2 *F Alexandrov DIS 70 *F neutrons, 10Gy *F 34Db -- 34Dg *F >*a Df(2L)TE35B-400 *F Roote *F white eyed derivative (SW400), selected as red-eyed fly from TE35B-SR100 *F homozygous *F noc -- Adh *F >*a Df(2L)TE35B-401 *F as TE35B-400 *F >*a Df(2L)TE35B-402 *F As TE35B-400 *F >*a Df(2L)TE35B-403 *F Coulson *F white eyed derivative, selected as red-eyed fly from TE35B-SR100 homozygous *F noc -- Adh *F >*a Df(2L)TE35B-407 *F Coulson *F white eyed derivative, selected as red-eyed fly from TE35B-SR100 homozygous *F 34Eb -- noc *F >*a In(2L)b79h1A *F This is probably the same as b79h1. Alexandrov originally sent us a stock *F labelled 79h1 which we chucked because it was a viable black, but he *F published b79h1 in DIS 63 as: *F Tp(2L)(sic)34D2-4;34D8-E1;41 *F In 1990 he sent 79h1 again - the cytology was In(2L)34D4;40E and *F genetically 34Db -- 34Dc *F >*a In(2L)b82a1 *F Df(2L)34B7-12;34E1.2 + In(2L)33C6-D1;34B7-12 *F base ch. uf3 pr cn *F gamma *F 34Da -- 34Eb *F >*a In(2L)b88e45 *F In(2L)34D2-4;40E-F Alexandrov *F Alex *F neutrons + gamma, 30Gy *F 34Db -- 34Dg *F >*a In(2L)b80c2 *F In(2L)34C7;34D6.7 *F base ch. Sco *F gamma *F b- *F >*a In(2L)b82c44 *F In(2L)34D4.5;40h *F Alexandrov DIS 63 *F gamma *F Sos -- b, but homozygous viable (sterile). *F >*a In(2L)Epa *F In(2L)25A1.4;35D1.2 *F Glynnis Johnson *F gamma *F base ch. b AdhF cn bw *F >*a In(2L)TE34Cc-1 *F In(2L)34B12;40 *F Roote *F spontaneous variegator of TE34Cc *F >*a In(2L)TE35B-400 *F In(2L)35B3-5;36D1.2 *F Roote *F red-eyed derivative (SR400), selected as red-eyed fly from TE35B-SR100 *F homozygous *F >*a In(2LR)b81a2 *F In(2LR)34E3;41E + Df(2L)34D5;34E3 *F Alexandrov DIS 63 (but published as Tp(2)b81a = Tp(2)34D2-4;34D8-E2;41D1-E1) *F gamma *F b -- 34Dc *F made autosynaptic so In(2LR) is correct. *F >*a In(2LR)b81a2LDTD43R *F Roote *F recombinant via autosynaptics between b81a2 and DTD43 *F b -- noc *F >*a In(2LR)DTD121 *F In(2LR)35B;41;42A;56F *F Gelbart *F gamma *F wbAM12 *F >*a In(2LR)DTD128LTE35B-226R *F Roote *F recombinant via autosynaptics between DTD128 (35A3.4;48C6-8) and *F TE35B-GR226 (35B;47B10-14;58F) *F new order 21-35A3.4|48C6.8 - 35B|47B10.14 - 60 *F elA -- noc *F >*a In(2LR)el6LA379R *F Roote *F recombinant (not using autos) between el6 and A379 *F new order 21 - 35B1.3|57C3.9 - 35B1.3|57A8.11 - 60 *F pu -- Adh *F >*a In(2LR)noc4LTE35B-50R *F recombinant via autosynaptics between noc4 and TE35B-50 (=GR50) *F noc- *F >*a In(2LR)TE35B-226LDTD128R *F don't think we ever succeeded in making this. *F >*a T(1;2;3)b75.1 *F T(1;3)1B7.8;62B9 + T(2;1)42E;1B7.8 +T(2;3)42E;62B9 *F Ashburner et al. January 1980 55:193- *F independent b allele *F x-ray induced *F >*a T(2;3)b83l2 *F T(2;3)34B12;83D3-5 + Df(2L)34C2;34E1.2 *F Alexandrov DIS 63 *F gamma *F 34Db -- 34Dg *F >*a T(2;3)GT10DTE35B-209P *F Durrant *F by segregation *F 34Fd -- noc *F putative new order 21 - 35A1-4|76A5-7 - 100; 60 - 35B|81 - 61 (breakpoint *F of TE35B-209 must be 81, not 80 as in L & Z) *F >*a T(2;3)shvS19Del24P *F Roote *F by segregation, selecting for pu *F elB -- elA *F putative new order 21 - 22F|35A - 22F|97A - 61A; 60 - 35B1.3|93C3-7 - 100 *F >*a T(2;3)TE35B-28Dosp90P *F Roote *F by segregation *F noc -- osp, snaAM15 *F new order 21 - 35B1.2|90C3-5 - 100; 60 - 35B3.4|89B9-11 - 61 *F >*a T(2;3)TE35B-28Dpb3P *F by crossing off second translocation from 2P region of pb3 and segregation *F of new element with TE35B-28 2D element. *F noc -- Adh *F new order 21 - 35B1.2|90C3-5 - 100; 60 - 35|89A9.10 - 100 *F >*a T(2;3)TE35B-3aDH16P *F Gubb *F by segregation *F noc -- 35Dd *F new order 21 - 35B1.2|86F12-87A1.2 - 61; 60 - 35D5.7|87F - 85F6.7|87F - 100 *F >*a T(2;3)TE35B-401 *F T(2;3)35B3.4;66B9.13 *F Roote *F red-eyed derivative (SR401), selected as red-eyed fly from TE35B-SR100 *F homozygous *F >*a T(2;4)GT6DTE35B-101P *F Roote *F by segregation *F 34Fc -- noc *F new order 21 - 34F3|101-102; 60 - 35B|101 - 102 *F >*a T(Y;2)a15 *F T(Y;2)BSYy+;35A4-B1.2 *F Lyttle *F base ch. In(2LR)Pu, SD-R1 *F mapped to el (=el5) *F >*a T(Y;2)A80DTE35B-18P *F Roote *F by segregation *F elA- *F >*a T(Y;2)b88g22 *F T(Y;2)Y;34D2-4 + T(2;3)34D2-4;87F8-12 + In(2R)41;52A14-B1 + *F Tp(3)69D2.3;92A14;93A6 Alexandrov *F Alexandrov DIS 70 *F neutrons + gamma, 30Gy *F Sos -- b *F >*a T(Y;2)el5DA80P *F Roote *F by segregation *F elB -- pu *F >*a T(Y;2)G74DA80P *F Df(2L)34C3;35A3.4 *F by segregation *F 34Da -- pu *F >*a Tp(2;2)b81f3 *F In(2L)34D2-4;35B10 Alexandrov *F Alexandrov DIS 63 *F gamma *F b- *F >*a Tp(2;Y)b88b15 *F Df(2L)34C3:35F2-12 (Ash) + Dp(2;Y)34F;35E1.2 Alexandrov *F Alexandrov DIS 70 *F neutrons, 2.5Gy *F Df 34Da -- BicC + Dp 34Fa -- BicC (i.e. Df 34Da -- rk) # *U FBrf0077942 *a Cohen *b S.M. *t 1995.4.19 *T personal communication to FlyBase *u *F From scohen@embl-heidelberg.de Wed Apr 19 09:55:43 1995 *F Date: Wed, 19 Apr 1995 10:55:54 +0100 *F From: Stephen Cohen *F Content-Transfer-Encoding: 7BIT *F To: ma11@gen.cam.ac.uk *F Subject: Re: Help FlyBase please about ey-Dominant *F To: 'ma11' *F X-Mailer: VersaTerm Link v1.1.1 *F Content-Length: 5908 *F *F Hi Michael, *F *F I'll see what I can do to help clarify things. *F *F the info on these breakpoints probabaly comes from a response sheet: *F > *F >T(2;3)AlpXTl, 24A1,2;80 *F >T(2;3)AlpDTD5, 24A1; 68F1,2 *F >Dp(2;4)AlpeyeD,23DE;24A1;102D *F >T(2;4)AlpXt2, 24A1;101D *F > *F >We believe there are no problems with 'T(2;3)AlpXTl', and that it is *F >the same as T(2;3)XT1 mentioned in the Alp entry in Lindsley and Zimm *F >(p24). It does not have its own entry in the Aberrations section of *F >L&Z; in FlyBase it is now T(2;3)Alp[XT1] ([] enclose superscripts). *F *F I agree *F *F > *F >We believe (slightly less confidently) that 'T(2;3)AlpDTD5' is the *F >aberration now known as T(2;3)DTD57, which was listed in L&Z under its *F >provisional name (in a Gelbart stock list) of T(2;3)434.128. *F *F correct *F *F > *F >The real 'fun' concerns the other two. The L&Z entry for Alp links *F >Alp[2] with an aberration T(2;4)X2; is this the 'T(2;4)AlpXt2' listed *F >above? *F yes *F *F T(2;4) Alp X2 and Dp (2;4) ey[D] are independently isolated chromosomes. the *F designations above are ok. *F *F As far as we can tell ey[D] is in fact two gain of functions -it shows an *F eyeless phenotype and it shows an Alp dominant phenoytpe. We believe that *F the Alp dominant is explainable on the basis of the transposition of *F material from the 24A1,2 breakpoint (as for the other Alp dominants). We *F have assumed, but not tested that the ey[D] phenotype is due to the site of *F insertion, since no other Alp dominant shows an eyeless phenotype. *F *F *F >To cap it all, we also have a listing for an allele Alp[XT2], which is *F >linked to a T(2;3) with breaks identical to those of T(2;3)Alp[XT1]. *F > *F this is wrong *F *F >In summary, here are my questions. Any help you can give, particularly *F >if you can cite published references, will help us a lot. *F *F All of this is unpublished, so you'll have to cite my unpublished data (I *F know we should publish it!) *F *F *F >(1) Is Alp sequenced? I have a suggestive note but no accession number. *F *F yes, but.... *F We have done a lot of work on this. We have found lack of function mutations *F and gain of function mutations. There are 6 independent gain of function *F alleles that break within 15 -20 kb in a region between 2 transcription *F units. The dominant phenotypes segregate with the distal arms of the *F breakpoints when we can test it (three unambiguous cases and ey[D]). *F Paradoxically, the lack of function mutant is associated with the *F transcription unit on the proximal side of the breakpoints. We have sequence *F the genes on both sides of the breakpoint(s). Both are homologous to odd *F skipped (the proximal one is the same as Doug Coulter's odd-related-gene). *F Niether is responsible for the Alp dominant phenoytpe. (we sequenced the *F coding region of the transcription unit distal to the breakpioint from a *F chromosome carrying an ems induced revertant of the dominant phenotpye- *F there were no changes in the aa seqeunce- therfore that can't be the gene). *F These data suggest that the gain of function is due to a regulatory element *F for leg expression that we know lies distal to the breakpoints of all *F rearrangements and that it has nothing, as such, to do with the genes on *F either side that were mutated to cause lack of function phenoytpes. *F *F I think that this is genetically interesting and that we should write it *F down, but I haven't really thought abouyt how to do it, in view of other *F time pressures. Any thoughts from you on whether this should be written down *F (and if so where to send it) would be welcome. *F *F *F >(2) What is the evidence that Alp is transposed onto cme4 in ey[D]? *F *F The evidence that the duplicated material in ey[D] is of 2nd chrosomsome *F origin (from the Alp region) comes from cytology by Gerd Jurgens. Gerd *F looked at a cross between ey [D] and T(2;4) Alp [X2], and saw pairing which *F revealed the 24A banding pattern (that we knew was associated with the *F T(2;4) Alp [X2] breakpoint). This suggested that the transposed material in *F ey[D] is of second chromosome origin. Also, the ey[D] chrosomome has an Alp *F dominant leg phenotype. This is not published anywhere as far as I can *F recall, and you have obviously checked. It can be cited as Cohen and Jurgens *F unpublished. We have also mapped the eye breakpoint moelecularly to our walk. *F *F >(3) The cytological information we have indicates that the insertion is a *F > reverse duplication of the material (Adelaide reports that cme4 looks *F > Y-shaped on account of the two inserted copies synapsing). Have you *F > any data on this point? *F *F no *F *F >(4) Is there evidence that Alp (or any other inserted gene) is mutant in *F > ey[D], as opposed to the other option that the entire phenotype is due *F > to disruption of cme4 genes (possibly ey, possibly others) and/or to *F > expression of the extra wildtype copy/ies of an inserted gene, eg Alp? *F *F The evidence that the Alp phenotype derives from the insertion of 2nd *F chromosome material is outlined above *F *F *F >(5) Is T(2;3)DTD57 an Alp mutation, specifically the one suggested above? *F *F yes. this is DTD57. It has an Alp dominant leg phenotype and the break is *F molecularly mapped. *F *F >(6) Is Alp[2] associated with a T(2;3), a T(2;4) or a Tp(2;4)? *F *F T(2;4)Alp [X2] is a reciprocal translocation stock. X2 cytology is 24A1,2 *F reciprocally translocated to 101D (cytology by Jurgens). It is distinct from *F Dp (2;4) ey[D]. *F *F *F >(7) Is Alp[XT2] the same as Alp[2]? If not, what is its associated aberr? *F *F this is the same thing *F *F >(8) Is the Alp allele on ey[D] (if any) either Alp[2] or Alp[XT2]? *F *F no, they are independent *F eyD is the original designation, you could perhaps call it Dp (2;4) ey[D], *F or Dp 2;4) Alp[eyD]. the former is more conservative, but the latter reflect *F that it is apparently two independent gain of function mutants. *F *F *F *F *F Hope this helps. I would welcome having the chance to discuss the data on *F this with you in some detail to see if we have a publishable story. As you *F see we've done a lot of work on it. Let me know what you think *F *F *F Best regards, *F *F Steve *F *F .. *F *F From Stephen.Cohen@embl-heidelberg.de Mon Apr 24 13:34:59 1995 *F Date: Mon, 24 Apr 1995 14:31:56 +0100 *F From: Stephen Cohen *F Subject: Re: Alp & ey[D] *F To: ma11 *F X-Mailer: VersaTerm Link v1.1.1 *F Content-Transfer-Encoding: 7BIT *F Content-Length: 524 *F *F At the time we were convinced that the transcript distal to the *F translocation bkpts had to be the Alp gene. As you see, subsequent analysis *F made it clear that this transcription unit cannot be the Alp GOF. I'd prefer *F if it is made clear that the transcript is the odd-like gene distal to *F org-1, and not refer to it as Alp, since that could be confusing. We would *F like to reserve the right to name it properly. We have mutants and are *F looking at some phenotypic stuff with Jackle, so it may get a 'proper' name *F soon. *F *F Steve *F *F .. *F *F From ma11@gen.cam.ac.uk Mon Apr 24 12:19:02 1995 *F From: Michael Ashburner (Genetics) *F Date: Mon, 24 Apr 95 12:21:11 BST *F To: Stephen.Cohen@embl-heidelberg.de *F Subject: Re: Alp & ey[D] *F Cc: ma11@gen.cam.ac.uk, ag24@gen.cam.ac.uk *F Content-Length: 743 *F *F *F Thanks Steve. I will contact Coulter about the org genes. We *F have org1 and also an org2, with no reference, said to be in *F 24C ! I guess this might be yr next distal transcript. *F *F This was curated from yr Mech Dev paper: is it wrong ? *F *F \*E FBrf0054617 == ew10.mg == Cohen et al., 1991, Mech. Dev. 33: 229--240 *F \*p @Alp@ is expressed in the embryo in a series of segmentally repeated *F \*p stripes that lie posteriorly adjacent to the en stripes in each segment. *F \*p In germ band retracted embryos @Alp@ is also expressed in a small cluster *F \*p of cells in the thoracic segments, and may be related to the appearance *F \*p of dorsal disc primordia. *F *F or should it be made clear that this refers to the pattern of the *F (unnamed) distal transcript ? *F *F Michael *F *F *F .. *F *F From ma11@gen.cam.ac.uk Wed Apr 19 08:45:56 1995 *F From: Michael Ashburner (Genetics) *F Date: Wed, 19 Apr 95 08:47:57 BST *F To: s.cohen@embl-heidelberg.de *F Subject: Help FlyBase please about ey-Dominant *F Cc: ma11@gen.cam.ac.uk, steveh@howard.fhcrc.org, ag24@gen.cam.ac.uk *F Content-Length: 3987 *F *F Dear Steve *F *F Steve -Steve Henikoff pointed out to us that there *F are problems with the FlyBase Alp/ey[D] records. After *F some detective work we think we now know just what the *F problems are. This is probably all a bit much, but any help *F you can give would be great. *F *F The start of the problem *F is that, in the distant pre-FlyBase past, I seem to have implicated you *F as the source of the belief that the 4th-cme insertion is of material *F including Alp. We have searched quite hard for published confirmation *F of this, by you or anyone else, without success. The best we have *F found is that I quoted you (personal comm.) in my Laboratory Handbook *F (p770) and that both John Merriam and I have notes to the effect that *F you announced this at the ADRC in Chicago in 1991 (though I see it is *F not mentioned in the abstract). *F *F The confusion is a good deal wider than that, unfortunately. Merriam's *F notes indicate that your ADRC poster (or possibly your reply to one of *F his request forms) mentioned the following aberrations; the frequency *F of typos in his files was vast, so I will err on the side of caution *F and quote verbatim. *F *F T(2;3)AlpXTl, 24A1,2;80 *F T(2;3)AlpDTD5, 24A1; 68F1,2 *F Dp(2;4)AlpeyeD,23DE;24A1;102D *F T(2;4)AlpXt2, 24A1;101D *F *F We believe there are no problems with 'T(2;3)AlpXTl', and that it is *F the same as T(2;3)XT1 mentioned in the Alp entry in Lindsley and Zimm *F (p24). It does not have its own entry in the Aberrations section of *F L&Z; in FlyBase it is now T(2;3)Alp[XT1] ([] enclose superscripts). *F *F We believe (slightly less confidently) that 'T(2;3)AlpDTD5' is the *F aberration now known as T(2;3)DTD57, which was listed in L&Z under its *F provisional name (in a Gelbart stock list) of T(2;3)434.128. *F *F The real 'fun' concerns the other two. The L&Z entry for Alp links *F Alp[2] with an aberration T(2;4)X2; is this the 'T(2;4)AlpXt2' listed *F above? The problem is that not only is T(2;4)X2 absent from the *F Aberrations section of L&Z, but there is instead a mention of Tp(2;4)X2 *F on p1058, which horrifyingly is stated as 'Mutant for Alp[2] and ey[D]'. *F *F You may recall that L&Z instituted a change in the definition of what *F was a translocation and what a transposition. This was incompletely *F implemented, and there are many cases where an aberration is called a *F Tp in its own entry but is still called a T when referred to in free *F text elsewhere. We have so far presumed that this is a case of that. *F However, the case is of course very weak, not least because the *F deficiency segregant from ey[D] is long lost (if it was ever recovered *F at all). We now suspect that two independent mutations have mistakenly *F been merged, both in L&Z and in FlyBase. *F *F To cap it all, we also have a listing for an allele Alp[XT2], which is *F linked to a T(2;3) with breaks identical to those of T(2;3)Alp[XT1]. *F *F In summary, here are my questions. Any help you can give, particularly *F if you can cite published references, will help us a lot. *F *F (1) Is Alp sequenced? I have a suggestive note but no accession number. *F (2) What is the evidence that Alp is transposed onto cme4 in ey[D]? *F (3) The cytological information we have indicates that the insertion is a *F reverse duplication of the material (Adelaide reports that cme4 looks *F Y-shaped on account of the two inserted copies synapsing). Have you *F any data on this point? *F (4) Is there evidence that Alp (or any other inserted gene) is mutant in *F ey[D], as opposed to the other option that the entire phenotype is due *F to disruption of cme4 genes (possibly ey, possibly others) and/or to *F expression of the extra wildtype copy/ies of an inserted gene, eg Alp? *F (5) Is T(2;3)DTD57 an Alp mutation, specifically the one suggested above? *F (6) Is Alp[2] associated with a T(2;3), a T(2;4) or a Tp(2;4)? *F (7) Is Alp[XT2] the same as Alp[2]? If not, what is its associated aberr? *F (8) Is the Alp allele on ey[D] (if any) either Alp[2] or Alp[XT2]? If not, *F does it have a published designation? (We currently call it Alp[eD].) *F *F Best wishes *F *F Michael # *U FBrf0077943 *a Appel *b L.F. *t 1995 *T personal communication to FlyBase *u *F From LAPPEL@eagle.wesleyan.edu Wed Apr 19 21:01:46 1995 *F Date: 19-APR-1995 15:46:01.85 *F From: LAPPEL@eagle.wesleyan.edu *F Subject: Sb *F To: flybase-help@morgan.harvard.edu *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 8503 *F *F Correction to FLYBASE information of Sb. *F *F Dear Flybase: *F *F After happily cruising Flybase for info on regions of interest to my *F current post-doctoral research, I have finally gotten around to *F looking up the subject of my PhD work, Sb-sbd. Much to my *F chagrin, I have discovered that the person in my old lab who was *F supposed to send in the new information to the Redbook *F consortium in 1992 never did. You have some information from *F our PNAS paper (FBrf0059263 == Appel et al., 1993, Proc. natn. *F Acad. Sci. USA. 90(11): 4937--4941), but there is more information *F in my thesis (Appel, L.F. (1992) Ph. D. thesis, University of *F California, Berkeley). *F *F First off, the cytological location Flybase uses for Sb is wrong. *F You & Redbook say *F 'Cytological map position : *F Placed in 89B4-5, probably in 89B4, by Lewis (1951). *F This probably corresponds to 89B9-10 on Bridges's *F revised map.' *F This is correct, but then Cytosearch uses the old version (89B4-5). *F It should use the new version (89B9-10). *F *F Under 'Other information' you say *F 'Authors consider that Sb and sbd are represented by the same transcript, *F although no discussion of this point is explicitly included.' *F The figure and discussion in the PNAS paper show that both sbd *F and Sb alleles are associated with DNA lesions within the same *F transcription unit. The issue is addressed explicitly in the thesis. *F *F The lethality of Sb1 is probably not due to Sb, but to a second *F mutation on the same chromosome called l(3)89Aa, identified by *F Nelson and Szauter. (FBrf0057262 Nelson, C. R. and Szauter, P. *F (1992). Cytogenetic analysis of chromosome region 89A of *F Drosophila melanogaster,. Mol. and Gen. Genetics 235, 11-21.) *F *F I have several notes for you about sbd26. *F First, the cytology of Df(3R)sbd26 is also incorrect, this time due to *F a typo. *F Flybase says: *F 'Aberration symbol : Df(3R)sbd26 *F Last updated : 21 Feb 95 *F Full name : Deficiency (3R) stubbloid *F FlyBase aberration id : FBab0002924 *F Breakpoints : 87C7-87D1;89B9-89B10' *F *F Later in the reference there are the correct breakpoints: *F 'Breakpoints : 89B9-89B10;89C7-89D1' *F It looks like 89 turned into 87 at some point during transcription. *F 89 is correct. *F In addition, the sbd and the deficiency are probably independent. *F The proximal breakpoint of the deficiency mapped at least 112 kb *F away from the nearest part of the transcript. *F More importantly, most stocks advertizing themselves as *F Df(3R)sbd26 are actually Df(3R)sbd45. (26 deletes from 89B9-10 *F distal, 45 deletes from 89B9-10 proximal.) This was true of stocks *F from every stock center I tried, including Indiana and Umea (and *F the stock we had in the Fristrom lab and had published based on *F previously). Luckily, Szauter still had the real thing. Outcrossing *F to ss- is diagnostic: Df(3R)sbd45 complements, Df(3R)sbd26 does *F not. If Indiana's stock dates from prior to 1992, it is probably *F wrong, and should be replaced with the Fristrom or Szauter lab's *F correct version. *F *F For sbd104, Flybase lists only *F 'Parent aberration : Tp(3;3)sbd[104] *F *F Aberration symbol : Dp(3;3)sbd[104] *F Last updated : 21 Feb 95 *F FlyBase aberration id : FBab0010059' *F Flybase should list the deficiency as well as the transposition, so it *F will come up under ' --- Deleted segments' *F *F *F For the rest of the alleles, I append here a table and paragraph *F from my thesis. It includes all Sb and sbd alleles I am aware of. I *F made serious efforts to get hold of SbW and the Spillman-Faller *F sbds other than 26 and 45 -- I am convinced that they no longer *F exist and Flybase should not get anyone's hopes up by listing them *F without asterisks. *F *F Table 3.1 Alleles of the Stubble-stubbloid locus. *F *F Allele (alternate NotesOrigin *F name) *F *F Stubble alleles *F Sb1 > Spontaneous; Bridges, 1923. *F Sb63b > Spontaneous; Merriam, 1963. *F Sb70 > Spontaneous. *F SbSpi (Spike) X-ray; Moore, 1931. *F SbV X-ray, T(2;3); Lewis, 1948. *F Variegates to +, progenitor Sb1. *F SbW * Found in the wild, Crow; no longer exists. *F *F stubbloid alleles *F sbd1 (Sbr) Spontaneous; Sturtevant, 1926. *F sbd2 Spontaneous; Harnley. *F sbd2Sb1 Recombination; Lewis, 1952. *F sbdlethal(sbdl) > X-ray; Lewis. associated with T(2;3) Me; TM1. *F sbd26 >! X-ray, Df; Spillman-Faller, 1978, *F sbd45 > X-ray, Df; Spillman-Faller, 1978. *F sbd## * Other sbd alleles generated by Spillman-Faller, *F all of which had had two-digit designations, *F no longer exist. *F sbd104 > X-ray, Tp(3:3); Lewis, 1947; also Df by *F recombination. *F sbd105 > X-ray, Df; Lewis, 1948. *F sbd106 > X-ray, T(2;3); Lewis. *F sbd201 (dtd 20-1) EMS; Beaton & Fristrom, 1986. *F sbd203 (sbd71) % Bevatron (Niobium, 500 rads); Hammonds, 1989. *F sbd204 (sbd89) % Bevatron (Niobium, 500 rads); Hammonds, 1989. *F sbd206 (sbd28) @ EMS; Hecht, 1989. *F sbd207 (sbdG73)$ Gamma irradiation; Gelbart, 1973. *F sbd208 (sbdlG) $ Spontaneous, McCarron. *F sbd210 (sbd91h)= Found in wild, Auburn Calif., M. Green, 1991. *F sbdGT11 >~ Gamma irrad., T(2;3); Ashburner. (Gubb et al.) *F sbdPNR11 ~ EMS; Heitzler and Simpson, 1991. *F sbdVX1 >~ X-ray; Heitzler and Simpson, 1991. *F sbdVE2 ~ EMS; Heitzler and Simpson, 1991. *F sbdVE3 ~ EMS; Heitzler and Simpson, 1991. *F *F Stubble Revertants (all are sbd) *F sbd202r (Sb63bR1, Rev1) >& DEB; Surh, Beaton and Fristrom, 1987. *F sbd205r (sbdC34) >& P-element (D2-3/Birm-2); Appel and *F Bayer, 1989. *F sbd209r (sbdF57) $ Spontaneous revertant of Sb1; McCarron. *F TM3 Sb1R+SE1 Ser ~ EMS; Heitzler and Simpson, 1991. *F TM3 Sb1R+SE2 Ser ~ EMS; Heitzler and Simpson, 1991. *F TM3 Sb1R+SX3 Ser >~ X-ray; Heitzler and Simpson, 1991. *F TM3 pnrD4 Sb1R+SX4 Ser ~ X-ray; Heitzler and Simpson, 1991. *F *F Other strains *F TM3 pnrD4 Sb1 Ser ~ Progenitor of pnrD4R+3 and Sb1R+SX3. *F TM3 pnrD4R+3 Sb Ser ~ X-ray; small Df just proximal to, but *F not altering, Sb1. Heitzler and *F Simpson, 1991. *F bxd100 > X-ray, Tp(3;3); Lewis; also available *F as Df by recombination; sbd+; just *F distal to Sb-sbd. *F *F *F Notes. *F *F > indicates DNA polymorphisms found in walk. *F \* indicates strains that have been lost over time. *F ! most strains called Df(3R)sbd26 in US and Europe are actually *F Df(3R)sbd45. Cross to ss- is diagnostic for real *F sbd26 deficiency, which may be independent of sbd26 *F mutation, see text. *F *F *F Source of mutants not found in Lindley and Zimm (1992): *F *F % Fristrom laboratory, UC Berkeley; progenitor red e. *F @ Anderson laboratory, UC Berkeley; progenitor rucuca. *F $ Chovnick laboratory, University of Connecticut. *F ~ Simpson laboratory, Faculte de Medecine, Strasbourg, France; *F progenitors st e or TM3 Sb Ser. *F & Fristrom laboratory, UC Berkeley; progenitor Sb63b. *F = Mel Green, UC Davis. *F *F Of the Sb alleles, Sb1, SbV, and SbSpi are much less severe in *F phenotype, both in bristle length and frequency and severity of *F malformation of appendages, than Sb63b and Sb70. sbd1, sbd2 and *F sbdG73 have much milder phenotypes than other sbd alleles. They *F are viable and not malformed as homozygotes. All other sbd *F alleles, including the Sb revertants, have shorter bristles than *F these three, and are malformed (though not every individual) as *F homozygotes or transheterozygotes. There are no viable alleles *F than can safely be called nulls. The two deficiencies which *F remove the entire gene, Df(3R)sbd45 and Df(3R)sbd105 are lethal *F both as homozygotes and as a transheterozygote. The *F Df(3R)sbd26/Df(3R)sbd105 transheterozygote gives viable *F malformed escapers, but as discussed earlier, the sbd26 mutation *F may be independent of the deficiency. The inversion that breaks *F the gene before the start of translation, In(3R)sbdVX1, has a more *F severe phenotype as a homozygote than over a deficiency, *F suggesting that it is not a true null. Both that inversion and the *F translocation T(2;3)sbdGT11 could have influences from promoters *F or enhancers in the foreign upstream DNA. The sbd205r insertion *F is also before the start of translation, but its transcript has not been *F sufficiently characterized to call it a null. All of the other mutants *F mapped could make truncated proteins. *F I hope this is of use. Let me know if you have questions, or want *F more information. You may reference this either to my thesis or *F as personal communication. *F *F Sincerely, *F Laurel F. Appel *F Dept. of Biology *F Shanklin and Hall-Atwater Laboratories *F Wesleyan University *F Middletown, CT 06459-0170 *F lappel@wesleyan.edu # *U FBrf0079728 *a Baumgartner *b S. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0079729 *a Britt *b S. *t 1995 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Apr 13 12:15:32 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 13 Apr 95 12:15:27 BST *F To: britt@uthscsa.edu *F Subject: Curation of 36th Ann. Dros. Conf. abstract 8B *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1065 *F Dear Dr. Britt, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 8B, discusses a novel opsin gene. In your abstract you *F neither give this gene a symbol or name, have you decided upon either? *F Do you know the cytological or genetic location of the gene or the *F function of the gene product? *F As this is a new gene the information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F Many thanks, *F Eleanor Whitfield. *F From BRITT@uthscsa.edu Thu Apr 13 17:04:52 1995 *F Date: Thu, 13 Apr 95 17:04:30 BST *F X-Sender: BRITT@thorin.UTHSCSA.EDU *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: BRITT@uthscsa.edu (STEVEN G. BRITT) *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 8B *F Content-Length: 943 *F Dear Eleanor, *F >Your abstract, 8B, discusses a novel opsin gene. In your abstract you *F >neither give this gene a symbol or name, have you decided upon either? *F Rh5: Rhodopsin 5 *F >Do you know the cytological or genetic location of the gene or the *F >function of the gene product? *F not yet mapped *F The Rh5 gene encodes a functional opsin, as demonstrated by *F electroretinogram analysis of ninaE mutant flies transformed with the Rh5 *F cDNA, under the control of the Rh1 (ninaE) promoter. *F >Curation of 36th Ann. Dros. Conf........ *F Sounds like a big job! It's great that 'y'all' are putting all of this *F stuff on the net. *F Good luck! *F Steve # *U FBrf0079730 *a Crews *b S. *t 1995 *T personal communication to FlyBase *u *F From eleanor Thu Mar 23 12:40:12 1995 *F To: steve_crews@unc.edu *F Subject: rho P-element enhancer trap *F Cc: eleanor *F Content-Length: 969 *F Dear Dr. Crews, *F I am a member of the FlyBase consortium working at the Cambridge, *F England, site under Prof. M. Ashburner. *F I am curating your paper: *F \*x FBrf0051386 == Nambu et al., 1990, Cell 63: 63--75 *F in which you discuss a P-element enhancer trap insertion into the rho *F locus, you give the symbol P[rho/lacZ]. *F Do you know the mutant allele created by this insertion as you give no *F name or previous references? *F This information is essential for complete curation of the publication *F so any help you can give is greatly appreciated. *F Many thanks, *F Eleanor Whitfield *F From steve_crews@unc.edu Mon Apr 10 14:12:17 1995 *F Date: Mon, 10 Apr 95 09:10:44 -0300 *F From: 'Steve Crews' *F Subject: Re: rho P-element enhancer trap *F To: 'Eleanor Whitfield (Genetics)' *F X-Mailer: VersaTerm Link v1.1.1 *F Content-Length: 651 *F Dear Eleanor, *F The rho/lacZ insertion described in the Nambu et al. paper was a novel *F insertion identified by my lab in an enhancer trap screen. The screen is *F briefly described in the publication, Crews et al. J. Exp. Zool. 261: *F 234-244 (1992). The name of the enhancer trap line is AA69 and this *F insertion is homozygous viable although occasional embryonic defects are *F observed. These defects have not been studied, nor is it confirmed that they *F are due to the P-element insertion. The rho/lacZ was used as an expression *F marker for rho, and not as a rho mutant in the Nambu et al. paper. I hope *F this answers your questions. *F Sincerely, *F Steve Crews # *U FBrf0079731 *a Fitzpatrick *b K.A. *t 1995 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Apr 27 08:28:02 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 27 Apr 95 08:27:56 BST *F To: kathleef@sfu.ca *F Subject: Curation of 36th Ann. Dros. Conf. abstract 113A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 949 *F Dear Kathleen Fitzpatrick, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 113A, discusses several PTP genes. In the database we *F have five protein tyrosine phosphatase genes. Could you tell me which *F of these, or if you used all of the Ptp genes in your study during *F oogenesis. *F Ptp4E, Ptp10D, Ptp36DE, Ptp61F or Ptp99A *F Any information you can give me will be greatly appreciated. *F Many thanks, *F Eleanor Whitfield. *F From kathleef@sfu.ca Thu Apr 27 17:31:25 1995 *F Date: Thu, 27 Apr 1995 09:17:44 -0700 *F Sender: kathleef@sfu.ca *F To: Eleanor Whitfield (Genetics) *F From: kathleef@sfu.ca *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 113A *F Content-Length: 831 *F Dear Eleanor Whitfield; *F The genes we have looked at during oogenesis are: DPTP61F, DPTP69D *F (this is the DPTP originally picked up by Saito along with DLAR), *F DPTP38A(DLAR), DPTP99A, DPTP10D, DPTP4E, DPTP36E (we have refined the *F position since our last presentation), and csw. The only phosphatases *F we didn't use are string and twine, whose oogenesis expression patterns *F are already known. *F I hope this is helpful to you in curating the abstracts. *F If there are any other questions, please do not hesitate to contact *F me. *F Kathleen Fitzpatrick. # *U FBrf0079733 *a Hacohen *b N. *t 1995 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Apr 13 11:44:37 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 13 Apr 95 11:44:34 BST *F To: hacohen@cmgm.stanford.edu *F Subject: Curation of 36th Ann. Dros. Conf. abstract *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1078 *F Dear Dr. Hacohen, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 23A, discusses two new genes sprouty and hypersprouty. *F In your abstract you do not assign symbols for these genes - have you *F decided upon the symbols yet? Do you know the cytological or genetic *F location of these genes or the function of the gene product? *F As these are new genes this information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F Many thanks, *F Eleanor Whitfield. *F From hacohen@cmgm.stanford.edu Sat Apr 22 06:19:03 1995 *F Date: Fri, 21 Apr 1995 22:15:24 -0700 *F To: Eleanor Whitfield (Genetics) *F From: hacohen@cmgm.stanford.edu (Nir Hacohen) *F X-Sender: hacohen@cmgm *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract *F Content-Length: 337 *F Hi Eleanor, *F sprouty (sty) is at 63D1,2 *F hypersprouty (hty) is at 61D1,2 *F Homozygous mutant embryos have twice the number of secondary branches in *F the tracheal system. *F I don't yet know the molecular functions of the two genes. *F Thanks for putting it all in Flybase. It's extremely useful for all us in *F the fly community. *F Nir Hacohen # *U FBrf0079735 *a Hopmann *b R. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079738 *a Lin *b T.Y. *t 1995 *T personal communication to FlyBase *u *F Dear Dr. Lin, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 137B, discusses a new gene mia. In your abstract you do *F not give the gene name for mia. Could you please tell me the name and *F other information regarding the cytological or genetic location of the *F gene or the function of the gene product if you know it. *F As this is a new gene the information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F Many thanks, *F Eleanor Whitfield. *F From beibei@leland.stanford.edu Wed Apr 19 23:05:00 1995 *F Date: Wed, 19 Apr 1995 15:02:39 -0700 *F From: Ting-Yi Lin *F To: eleanor@gen.cam.ac.uk *F Subject: Information about mia *F Content-Length: 939 *F Hi! Ms. Whitfield, *F The full name for mia is meiosis I arrest, and it was originally isolated by *F J. Hackstein in a screen for EMS induced male steriles. The original name *F given by Dr. Hackstein was ms(3L)570 and was renamed by Dr. Minx Fuller. Work *F done in the Fuller lab indicated that the mia mutation mapped to 3-47.0 +/- *F 0.2 m.u. based on 14 recombinants between the visible markers ri and Ki. It *F was located in polytene intervals 78C9-81F or 83A-C2 based on complementation *F by Df(3L)ri79c, Df(3L)Pc-MK, Df(3R)2-2, and Df(3R)Tpl10. The function of *F the gene product is unknown. The mutation causes a complete arrest of *F spermatogenesis at the G2/M transition to meiosis I, similar to aly, can, and *F sa. *F I hope the above information is helpful to you in updating the Flybase. And *F thank you so much for keeping the Flybase current and useful for all of us! *F Ting-Yi Lin *F c/o Dr. Minx Fuller *F beibei@leland.stanford.edu *F (415) 723-9703 # *U FBrf0079739 *a Lindsley *b D. *t 1993 *T personal communication to FlyBase *u *F Archived # *U FBrf0079743 *a Mechler *b B. *t 1995 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Apr 13 12:18:42 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 13 Apr 95 12:18:35 BST *F To: DEV.GENETICS@dkfz-heidelberg.de *F Subject: Curation of 36th Ann. Dros. Conf. abstract 16A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1079 *F Dear Dr. Merdes, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 16A, discusses a new nonmuscle myosin II heavy chain *F gene. In your abstract you do not give this gene a symbol, have you *F decided upon one? Do you know the cytological or genetic location of *F the gene or the function of the gene product? *F As this is a new gene the information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F Many thanks, *F Eleanor Whitfield. *F From Dev.Genetics@dkfz-heidelberg.de Fri Apr 14 18:18:33 1995 *F From: 'Bernard Mechler' *F Organization: DKFZ Heidelberg *F To: 'Eleanor Whitfield (Genetics)' *F Date: Fri, 14 Apr 1995 19:15:59 GMT+1 *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 16A *F Cc: eleanor@gen.cam.ac.uk *F Priority: normal *F X-Mailer: Pegasus Mail/Windows (v1.22) *F Content-Length: 268 *F Dear Dr. Whitfield, *F The nonmuscle myosin II heavy chain mentioned in the abstract of *F Merdes et al. of the American Drosophila Research Conference held at *F Atlanta refers to the already known zipper gene. *F Yours sincerely, *F Bernard Mechler # *U FBrf0079747 *a Nichols *b R. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0079755 *a Padgett *b R.W. *c W.M. *d Gelbart *t 1995 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Thu Mar 23 13:25:44 1995 *F Date: Thu, 23 Mar 95 08:26:21 EST *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: rd120@gen.cam.ac.uk *F Subject: Re: and now for something completely different *F Cc: flybase-updates@morgan.harvard.edu, gelbart@morgan.harvard.edu, *F padgett@ocelot.rutgers.edu *F Content-Length: 934 *F Hi Rachel, *F In(2LR)CyO-P20 is a CyO derivative in which a 20 kb transposon from *F ca. 72 to ca. 92 on the molecular map of dpp (St. Johnston et al., 1990) *F that covers all functions mediated by the dpp[Hin] and dpp[shv] region *F is inserted into CyO (polytene insertion position unknown). The 20 kb *F fragment is from a SalI digest of genomic DNA (I think from our dpp[+] *F dp cn bw isogenic strain that was also used as the RNA source for Nick *F Brown's cDNA libraries). *F In(2LR)CyO-P20 completely suffices for dpp function, even in the absence *F of any other copies of dpp in the genome. (In other words, it acts as *F if it is disomic for the dpp[Hin+] function.) *F This construct was made by Rick Padgett while he was a postdoc in my *F laboratory. I would like this personal communication to be cited *F as Padgett and Gelbart, pers. comm. to FlyBase. *F I am cc'ing this message to Rick in case he wishes to add to or amend *F anything I wrote. *F Bill # *U FBrf0079759 *a Posakony *b J.W. *t 1995 *T personal communication to FlyBase *u *F Date: Fri, 3 Feb 1995 11:27:05 +0000 *F From: Jim Posakony *F To: Cahir O'Kane *F Subject: Re: 62A *F Dear Cahir, *F Hilary Ellis, a former postdoc who generated several deficiencies in the *F emc region when she was in my lab, has deposited a number of them -- *F including Df(3L)14-8 -- into the Bloomington stock center. I understand *F that the stock center lists it as Df(3L)emc5. If for any reason you have *F trouble getting it from Bloomington (we don't carry it here anymore), you *F can still get it from Hilary, who's at Emory University and is listed in *F Flybase. In her stocklist, it's stock number H5. *F Good luck, and let me know if you need anything else. *F Jim # *U FBrf0079771 *a Scott *b M. *t 1995 *T personal communication to FlyBase *u *F From scott@rascal.med.harvard.edu Thu Mar 23 17:45:49 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 23 Mar 1995 12:48:34 -0500 *F To: rd120 *F From: scott@rascal.med.harvard.edu (Matthew Scott) *F Subject: Re: helping FlyBase *F Content-Length: 309 *F Dear Rachel, *F We have not to my knowledge figured out *F the lesion in Ns + RC3. The complexities of Ns itself make any such *F analysis a substantial undertaking. *F Wish I could help more-- *F Best wishes, *F Matthew # *U FBrf0079774 *a Skoulakis *b E. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0079776 *a Smith *b T.A. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0079787 *a Volk *b T. *t 1995.4.17 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Apr 13 11:59:38 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 13 Apr 95 11:59:36 BST *F To: lgvolk@weizmann.weizmann.ac.il *F Subject: Curation of 36th Ann. Dros. Conf. abstract 39B *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1047 *F Dear Dr. Volk, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 39B, discusses a new gene groovin. In your abstract you *F do not assign a symbol for this gene - have you decided upon a symbol *F yet? Do you know the cytological or genetic location of the gene or *F the function of the gene product? *F As this is a new gene the information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F Many thanks, *F Eleanor Whitfield. *F From LGVOLK@weizmann.weizmann.ac.il Mon Apr 17 14:16:29 1995 *F Date: Mon, 17 Apr 95 16:03:40 +0300 *F From: LGVOLK@weizmann.weizmann.ac.il *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 39B *F To: 'Eleanor Whitfield (Genetics)' *F Content-Length: 495 *F Dear Dr Eleanor Whitfield, *F In response to your request, here is some information concerning the *F groovin ge ne: *F 1. The cytological location is 50C3/4. *F 2. The symbol is grv. *F 3. As to the function of groovin, there is not yet mutation, but our *F antisense experiments suggest that it is important in a correct apodeme *F function to induce appropriate muscle pattern. The protein was *F mentioned in a 'Development' paper Development 120 59-70 (1994) Volk *F and VijayRaghavan. *F Sincerely yours, Talila Volk. # *U FBrf0079793 *a Zurita *b M. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0079811 *a Heisenberg *b M. *t 1995.5.3 *T personal communication to FlyBase *u Personal communication to FlyBase, 3rd May 1995. *F Archived # *U FBrf0079812 *a Gibson *b J. *t 1995.5.3 *T personal communication to FlyBase *u *F Fax from J. Gibson to FlyBase, 3 May 1995. *F Copy available from FlyBase on request. # *U FBrf0079813 *a Ellis *b H. *t 1995.5.7 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Sun May 7 14:07:01 1995 *F From: Aubrey de Grey (Genetics) *F Date: Sun, 7 May 95 14:07:10 BST *F To: hellis@biology.emory.edu *F Subject: query about some emc aberrations *F Cc: ag24@gen.cam.ac.uk *F Content-Length: 1028 *F *F Dear Dr. Ellis, *F *F We have found some anomalies in our records for some aberrations you *F isolated some years ago, and wonder if you could help. *F *F (1) The Bloomington stock centre has three deficiencies for emc, which *F they call Df(3L)emc5, emc18 and emc24. Do you know where these names *F come from? We know that emc5 is the same as Df(3L)Ac14-8 cited in the *F 1993 Genetics paper by Castrillon et al., and we understand that 14-8 *F was your original isolation number. Have there been any other mentions *F of any of these three in the literature, to your knowledge? *F *F (2) emc[19], aka 29-2, is noted in the Red Book (and thence in FlyBase) *F as associated with a T(2;3). The breakpoints, unfortunately, are given *F as 5B5-9;61C4. Is it in fact a T(1;3) or is there a digit missing from *F the cytology? *F *F Many thanks in advance, *F *F Aubrey de Grey *F FlyBase *F *F From hellis@biology.emory.edu Sun May 7 19:33:03 1995 *F To: ag24@gen.cam.ac.uk *F From: hellis@biology.emory.edu *F Organization: Emory University Biology Dept. *F Date: 7 May 1995 13:32:46 EST *F Subject: Re: query about some emc aberrations *F Priority: normal *F X-Mailer: Pegasus Mail/Mac v2.02 *F Content-Length: 1157 *F *F 1) Sorry about the confusion with the deficiencies. I hadn't noticed *F how they were named. It seems that Kathy named the deficiencies I sent *F her by my stock list #s (H5, H18, H24). This obviously won't do. The *F stock that Bloomington calls Df(3L)emc5 is indeed the same as what the *F Castrillon paper calls Df(3L)Ac14-8. 14-8 is my isolation # but I'm *F not sure where they came up with Ac. Apparently they got the stock *F from Jim Posakony. Each of these deficiencies were isolated as *F revertants of emc[D], aka Ach (sorry my email program doesn't do *F superscripts). My nomenclature (never published) was Df(3L)Ar14-8 (for *F B's Df(3L)emc5), Df(3L)Ar12-1 (for B's Df(3L)emc18) and Df(3L)Ar11 (for *F Df(3L)emc24 ). I think this would be a reasonable nomenclature since *F people who received the stocks from me will have my nomenclature. I *F don't know of any other publications that used these deficiencies. *F However, I have sent them to a number of labs. *F *F 2) emc[19] is T(1;3)5B5-9; 61C4 *F *F 3) If it hasn't been changed yet, the cytological location for emc *F should be 61D1-2 (by in situ localization of an emc cDNA probe) *F *F Regards, *F *F Hilary Ellis *F *F *F *F Dear Kathy, *F *F Here are the deficiency stocks. The numbers on the vials are my lab *F stock number. The cytology and genotypes are below. I have not checked *F the cytology in several years, although other labs have used them during *F that period. Note that they all should have an emc phenotype. Let me *F know if you have any problems with them. *F *F Sincerely, *F *F Hilary Ellis *F *F H5 - Df(3L)61C3/4,62A8, red/TM2, emc[2] p[p] Ubx e[s] *F H18 - Df(3L)61C,61F, red/TM2, emc[2] p[p] Ubx e[s] *F H24 - Df(3L)61C3/4,61E, red/TM2, emc[2] p[p] Ubx e[s]' # *U FBrf0079814 *a Weinzierl *b R.O.J. *t 1995.5.12 *T personal communication to FlyBase *u *F Fax from R. Weinzierl May 12 1995. *F Copy available from FlyBase on request # *U FBrf0079815 *a Vigneron *b M. *t 1995.5.18 *T personal communication to FlyBase *u *F From vigneron@titus.u-strasbg.fr Thu May 18 10:17:21 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-Mailer: Eudora F1.4.3 *F Date: Thu, 18 May 1995 11:16:43 +0100 *F To: m.ashburner@gen.cam.ac.uk *F From: vigneron@titus.u-strasbg.fr (MarcV) *F Subject: Drosophila RNA polymerase *F Content-Length: 1037 *F *F Dear prof. Ashburner, *F *F We described the drosophila cDNA encoding a subunit homologous to the ABC23 *F subunit encoded by the RPB6 gene in the yeast Saccharomyces cerevisiae, *F whose RNA polymerases, as you probably know, are the best described. This *F sequence was cloned by homology to the human cDNA hRPB14.4 that we have *F cloned as well. *F *F All these homologous subunits share a extremely well conserved C-terminal *F domain, fused to a more variable acidic N-terminal part. *F *F This subunit is encoded by a single locus in the human genome suggesting *F that the corresponding gene may be unique. In yeast indeed, a unique RPB6 *F gene encodes the corresponding subunit that is shown to be shared by the *F three types of RNA polymerases I/A, II/B, III/C. *F It is likely that this subunit will be shared by the three types of *F polymerases in all eukaryotes. *F *F The description of this sequence should appear soon in our next publication *F which was accepted in MCB. *F *F Please, feel free to contact me for any further informations. *F *F Sincerely yours, *F *F Marc Vigneron # *U FBrf0079816 *a Gelbart *b W.M. *t 1995.5.17 *T personal communication to FlyBase *u *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: kmatthews@morgan.harvard.edu, mashburner@morgan.harvard.edu *F Subject: Re: Dp(2;1)G146 *F Cc: gelbart@morgan.harvard.edu, tkaufman@morgan.harvard.edu *F Content-Length: 580 *F *F Dp(2;1)G146 was radiation induced as a detachment of C(1)RM, y2 su(wa) *F wa bb / T(Y;2)G146. I have not re-examined the breakpoints. L&S et al *F reported them as 23B-C. I believe Michael revised this to 23D-E, although *F I no longer see this in the aberration report of T(Y;2)G146. *F *F From gelbart@morgan.harvard.edu Wed May 17 18:19:02 1995 *F Date: Wed, 17 May 95 13:19:13 EDT *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: ma11@gen.cam.ac.uk *F Subject: Re: Dp(2;1)G146 *F Cc: gelbart@morgan.harvard.edu, kmatthews@morgan.harvard.edu *F Content-Length: 582 *F *F Michael, *F *F Yes, it needs a new record. Should it officially be a Dp(Y;2:1), which *F is a more accurate description, or a Dp(2;1), which is the way it is *F utilized? *F *F I made it with my own two little hands. *F *F The new order is of course something like: *F *F 1Lt -- Xhet | Yhet -- Yhet | 23B -- 2Lt *F *F ^ ^ *F | | *F | | *F new junction progenitor T(Y;2)G146 *F junction *F *F I need to find my notebook entry to check if it was X-ray or gamma-ray *F induced. *F *F Bill # *U FBrf0079817 *a Bryant *b P. *t 1989.2.22 *T personal communication to FlyBase *u *F Personal communication to Bloomington Stock Center from Peter Bryant: *F Tp(2;Y)cb50 is a male-fertile insertional translocation of regions 31-33 *F into the Y chromosome, generated by Terry Lyttle (D.I.S. 65, 213 but *F breakpoints are 30C; 33E not 30C; 30E as listed). It rescues the dominant *F sterility of Df(2L)J-der-39. # *U FBrf0079818 *a Vize *b J. *t 1995.6.6 *T personal communication to FlyBase *u *F Personal communication from: Janice Vize *F To: Bloomington Drosophila Stock Center *F Dated: 6 Jun 1995 *F Information communicated: The left breakpoint of Df(3L)Ac14-8 (synonym *F Df(3L)emc5) is proximal to 61C4-7 because the deficiency does *F not uncover the marbles gene at 61C4-7. # *U FBrf0079819 *a Roote *b J. *t 1995.5.4 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed Mar 29 17:17:25 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 29 Mar 95 17:17:18 BST *F To: jr32@phx.cam.ac.uk *F Subject: Genetics 126: 679 help please *F Cc: eleanor@gen.cam.ac.uk *F Content-Type: X-sun-attachment *F Content-Length: 18650 *F *F Hi John, *F *F Finally here is a list of the alleles I need help with, they are not *F given in the paper and are not in L&Z. *F *F ck has 16 alleles, 13 in L&Z so I need 3 more. *F Only 4 chif alleles in L&Z, need the fifth *F cni is not in L&Z, need two alleles. *F l(2)35Ce has only 3 alleles in L&Z, need two more *F l(2)35Cb has 6 alleles in L&Z, two are mentioned in paper but need one *F more, also is allele 5 (as in paper) or allele 6 (as in L&Z) associated *F with In(2L)shv22? *F l(2)35Cf is not in L&Z, need two alleles. *F l(2)35Dd has eight alleles, 4 are in L&Z, one extra is mentioned, need *F 3 more. *F l(2)35Dh is not in L&Z, need 1 allele. *F l(2)35Di is not in L&Z, need two alleles. *F l(2)35Fa is not in L&Z, need 14 alleles. *F l(2)35Fb is not in L&Z, need 3 alleles. *F l(2)35Fc is not in L&Z, need 2 alleles. *F l(2)35Fd is not in L&Z, need 1 alleles as one is named in paper. *F l(2)35Fe is not in L&Z, need 2 alleles. *F l(2)35Ff is not in L&Z, need 1 allele. *F l(2)35Cd has 9 alleles, 8 in paper, one more needed (5 in L&Z) *F lace has 21 alleles, 12 discussed, 5 extra are in L&Z so I need the *F remaining 4 *F BicC has 12 alleles, 2 in paper, 3 more in L&Z so I need 7. *F l(2)35Df has 6 alleles, 1 in paper, 3 more in L&Z so need 2 extra *F l(2)35Dg has 13 alleles, 2 in paper, 2 more in L&Z so need 9 more *F fzy has 5 alleles, 2 in paper, 2 more in L&Z so need 1 more *F sna has 23 alleles, 6 in paper, 14 more in L&Z so need 3 more *F *F Find attached a file of a record of the publication, ie the genes and *F alleles that are present in the record to help you. *F *F Any help you can give will be given as a pers comm to FlyBase. *F *F Many thanks, *F Eleanor *F *F From jr32@mole.bio.cam.ac.uk Thu May 4 11:54:13 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 4 May 1995 11:50:49 +0100 *F To: Eleanor Whitfield (Genetics) *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: Genetics 126: 679 help please *F Content-Length: 2986 *F *F Dear Eleanor, *F *F ck: *F *F I know about 18 alleles of ck, 5 more than in L & Z: *F *F T(2;3)H67 gamma induced by Hilliker *F TE35BC Ising *F GJ2 EMS by Glynnis Johnson *F A11 EMS by Pat Simpson *F IK3 spontaneous from Istvan Kiss (recent) *F *F chif: *F *F The extra allele is called A507. Origin: P{larB ry[+]} insertion on CyO, *F from Clive Wilson. *F *F cni: *F *F AR55 EMS induced by Roth. It is now called cni[1]. *F Sorry, don't know about any more. Nusslein-Volhard reported 2 alleles. *F *F 35Ce: *F *F 35Ce = escargot. There are millions of alleles, some of which are: *F *F \*k esg \*j VS2 \*l cn bw sp \*f Pat Simpson \*e EMS \*m l(2)br43, l(2)35Ce \# *F \*k esg \*j VS4 \*l cn bw sp \*f Pat Simpson \*e EMS \*m l(2)br43, l(2)35Ce \# *F \*k esg \*j VS8 \*l cn bw sp \*f Pat Simpson \*e EMS \*m l(2)br43, l(2)35Ce \# *F \*j (not sure of allele name. See Hayashi 1993 Dev 118:105-115) \*k esg \*e P *F element \*f Shirras \*m l(2)br43, l(2)35Ce \# *F \*b 35D1.2 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*k esg \*l cn *F \*b 35D1.2 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*k esg \*l cn \*m l(2)br43 *F , l(2)35Ce \*q 7082 \*f Spradling, Baltimore \# *F \*b 35D1.2 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*k esg \*l cn *F \*q 1/9 \*b 35D1-2 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35C *F e, l(2)br43 \# *F \*q 4/18 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 11/7 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 27/6 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 44/8 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 52/10 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 55/17 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 78/8 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 79/2 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 81/4 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 84/18 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 85/5 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 86/10 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 89/10 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 108/11 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 141/4 \*b 24D5-6 and 35D1-2 (cluster of 2) \*d PlacW \*f Kiss, Szeged \*k *F esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 154/18 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 158/18 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*q 161/17 \*d PlacW \*f Kiss, Szeged \*k esg \*l wild-type \*m l(2)35Ce, l(2)br43 \# *F \*j 80 \*d P \*f Tolias \*k esg \*m l(2)35Ce, l(2)br43 \# *F \*j 94 \*d P \*f Tolias \*k esg \*m l(2)35Ce, l(2)br43 \# *F \*j 148 \*d P \*f Tolias \*k esg \*m l(2)35Ce, l(2)br43 \# *F *F l(2)35Cb = shuttlecraft (stc). *F *F I don't know what the 7th allele was - but now there are 2 new ones. *F *F \*j A12 \*k l(2)35Cb \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br33 \# *F \*j B12 \*k l(2)35Cb \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br33 \# *F \*j B21 \*k l(2)35Cb \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br33 \# *F \*j HG38 \*k l(2)35Cb \*e EMS \*l Adh$supn10$esucn vg \*m l(2)br33 \# *F \*j AM9 \*k l(2)35Cb \*o In(2L)shv22 \*e X-rays \*f Gelbart \*m l(2)br33 \# *F \*j A14 \*k l(2)35Cb \*l b pr cn bw \*f Simpson \*m l(2)br33 \# *F \*b 35C1.2 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ05441 \*k l(2)35Cb \*l cn *F \*m l(2)br33 \*q 5441 \*f Spradling, Baltimore \#. *F \*j PW111/12 \*q 111/12 \*b 35D1-4 and 85D17-18 \*d PlacW \*f Kiss, Szeged \*k *F l(2)35Cb \*l wild-type \*m l(2)br33 \# *F *F l(2)35Cf *F *F \#381 and \#481 - but from the same experiment and may have been a cluster. *F *F l(2)35Dd = cycE *F I have found 4 'old alleles'. Can't find another 4, but there are 7 new ones! *F *F \*j GE1 \*k l(2)35Dd \*e X-rays \*o T(2;3)G16,ho2 \*f Gelbart \*l \*m l(2)br37 \# *F \*j P28 \*k l(2)35Dd \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br37 \# *F \*j P41 \*k l(2)35Dd \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br37 \# *F \*k l(2)35Dd \*j TE35D \*e Insert \*f Ising \*m l(2)br37 \# *F \*b 35D3-5 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ05206 \*k l(2)35Dd \*l *F cn \*m l(2)br37 \*q 5206 \*f Spradling, Baltimore \# *F \*b 35D3-5 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ05277 \*k l(2)35Dd \*l *F cn \*m l(2)br37 \*o probably cluster-mate of PZ7 \*q 5277 \*f Spradling, *F Baltimore \# *F \*b 35D3-5 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ05278 \*k l(2)35Dd \*l *F cn \*m l(2)br37 \*o probably cluster-mate of PZ6 \*q 5278 \*f Spradling, *F Baltimore \# *F \*b 35D3-5 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ10427 \*k l(2)35Dd \*l *F cn \*m l(2)br37 \*q 10427 \*f Spradling, Baltimore \# *F \*j PW8/7 \*q 8/7 \*d PlacW \*f Kiss, Szeged \*k l(2)35Dd \*l wild-type \*m l(2)br37 \# *F \*j PW50/7 \*q 50/7 \*b 35D3-4 \*d PlacW \*f Kiss, Szeged \*k l(2)35Dd \*l *F wild-type \*m l(2)br37 \# *F \*j PW91/9 \*q 91/9 \*d PlacW \*f Kiss, Szeged \*k l(2)35Dd \*l wild-type \*m *F l(2)br37 \# *F *F NB T(2;3)H16 has been renamed as T(2;3)G16 *F TE116 = TE35D! *F *F l(2)35Dh *F *F \*j AS64 \*k l(2)35Dh \*l b pr cn bw \*f Roth \*e EMS \# *F *F l(2)35Di *F only one allele: *F *F \*j RAR8 \*k l(2)35Di \*l b pr cn bw \*f Roth \*e EMS \# *F *F l(2)35Fa *F *F I know of only 4 alleles, one of which is very recent: *F *F \*j X7 \*k l(2)35Fa \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j X10 \*k l(2)35Fa \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AR66 \*k l(2)35Fa \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j IK2 \*q 31/5 \*b 57A and 85C \*k l(2)35Fa \*f Kiss, Szeged \# *F *F l(2)35Fb *F *F i know of only 1: *F *F \*j AS96 \*k l(2)35Fb \*f Roth \*e EMS \*l b pr cn wx bw \# *F *F l(2)35Fc *F *F I know of 1 'old' allele and 2 new ones: *F *F \*j AR144 \*k l(2)35Fc \*f Roth \*e EMS \*l b pr cn wx bw \# *F \*j PW114 /3*q 114/3 \*b 35F6-7 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fc \*l *F wild-type \# *F \*j PW124/1 \*q 124/1 \*b 35F6-7 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fc \*l *F wild-type \# *F *F l(2)35Fd *F *F one old allele and many new ones: *F *F \*j RAR46 \*k l(2)35Fd \*l b pr cn bw \*f Roth \*e EMS \# *F \*b 35F1.2 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ00232 \*k l(2)35Fd \*l *F cn \*m \*q 0232 \*f Spradling, Baltimore \# *F \*j PZ03101 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*k l(2)35Fd \*l cn \*m \*q *F 3101 \*f Spradling, Baltimore \# *F \*j PZ06872*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*k l(2)35Fd \*l cn \*m \*q *F 6872 \*f Spradling, Baltimore \# *F \*j PW8/9 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW55/19 (cluster of 2)35Fd \*d PlacW \*f Kiss, Szeged \*k l(2) \*l wild-type \# *F \*j PW56/1 \*b 35F4-5 (cluster of 9)35Fd \*d PlacW \*f Kiss, Szeged \*k l(2) \*l *F wild-type \# *F \*j PW62/7 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW80/7 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW88/6 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW92/14 (cluster of 3)35Fd \*d PlacW \*f Kiss, Szeged \*k l(2) \*l wild-type \# *F \*j PW97/4 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW104/15 \*b 35F1-2 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW110/24 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW136/11 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW161/29 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F \*j PW216/2 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \#. *F \*b 35F1-2 \*j PW78/29 \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \*d PlacW \# *F \*b 35F4-5 \*j PW58/14 \*f Kiss, Szeged \*k l(2)35Fd \*l wild-type \# *F *F l(2)35Fe *F *F one old, one new: *F *F \*j AS63 \*k l(2)35Fe \*f Roth \*e EMS \*l b pr cn wx bw \# *F \*j PW146/8 \*b 35F11-12 \*d PlacW \*f Kiss, Szeged \*k l(2)35Fe \*l wild-type \# *F *F l(2)35Ff *F *F \*j AR113 \*k l(2)35Ff \*f Roth \*e EMS \*l b pr cn wx bw \# *F *F l(2)35Cd *F *F I only know of 8 old alleles and 2 new ones: *F *F \*j B8 \*k l(2)35Cd \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br34 \# *F \*j B14 \*k l(2)35Cd \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br34 \# *F \*j HG39 \*k l(2)35Cd \*e EMS \*l Adh$supn10$esucn vg \*m l(2)br34 \# *F \*j HG43 \*k l(2)35Cd \*e EMS \*l b Adh$supF$esupr \*m l(2)br34 \# *F \*j VT7 \*k l(2)35Cd \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br34 \# *F \*j P34 \*k l(2)35Cd \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br34 \# *F \*j VT7 \*k l(2)35Cd \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br34 \# *F \*j d577 \*k l(2)35Cd \*l b cn \*f Goldsmith \*e P element \*m l(2)br34 \# *F \*b 35D1-4 \*d PZ (= P[lacZ, hsp70, ry$sup+$esu]) \*j PZ06430 \*k l(2)35Cd \*l *F cn \*m l(2)br34 \*q 6430 \*f Spradling, Baltimore \# *F \*j IK1 \*q 6/10 \*f Kiss, Szeged \*k l(2)35Cd \*l wild-type \*m l(2)br34 \# *F *F lac *F *F I know of 16 old alleles and 7 new: *F *F \*j AM1 \*k lac \*e spontaneous \*l Su(H) l(2)Su(H) whd \*m l(2)br36, l(2)35Dc \# *F \*j HG34 \*k lac \*e EMS \*l Adh[n7] cn (vg) \*m l(2)br36, l(2)35Dc \# *F \*j P42 \*k lac \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j TB8 \*k lac \*e X-rays \*o T(Y;2)TB8 \*f Lyttle \*m l(2)br36, l(2)35Dc \# *F \*j VM1 \*k lac \*e EMS \*l \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VM2 \*k lac \*e EMS \*l \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT1 \*k lac \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT2 \*k lac \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT3 \*k lac \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT4 \*k lac \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT5 \*k lac \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT6 \*k lac \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT8 \*k lac \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT9 \*k lac \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT10 \*k lac \*e EMS \*l b cn bw \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j VT11 \*k lac \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br36, l(2)35Dc \# *F \*j IRX6 \*k lac \*f Simpson \*m l(2)br36, l(2)35Dc \# *F \*j PW7/6 \*b 35D1-4 and 46A1-2 \*d PlacW \*f Kiss, Szeged \*k lac \*l wild-type *F \*m l(2)35Dc, l(2)br36 \# *F \*j PW23/3 \*b 35D3-4 \*d PlacW \*f Kiss, Szeged \*k lac \*l wild-type \*m *F l(2)35Dc, l(2)br36 \# *F \*j PW53/5 \*b 35D3-4 \*d PlacW \*f Kiss, Szeged \*k lac \*l wild-type \*m *F l(2)35Dc, l(2)br36 \# *F \*j PW75/1 \*b 35D3-4 \*d PlacW \*f Kiss, Szeged \*k lac \*l wild-type \*m *F l(2)35Dc, l(2)br36 \#. *F \*j PW82/8 \*b 24C1-2 and 35D1-2 \*d PlacW \*f Kiss, Szeged \*k lac \*l wild-type *F \*m l(2)35Dc, l(2)br36 \# *F \*j PW113/9 \*b 35D1-2 and 54B12-16 \*d PlacW \*f Kiss, Szeged \*k lac \*l *F wild-type \*m l(2)35Dc, l(2)br36 \# *F *F BicC *F *F 11 alleles: *F *F \*j AA29 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AR108 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AA4 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AB74 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AB79 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AR72 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j AR96 \*k BicC \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j WC45 \*k BicC \*f Schupbach \*l cn bw sp \# *F \*j YC33 \*k BicC \*f Nusslein-Volhard \# *F \*j PE37 \*k BicC \*f Schupbach \*l cn bw \# *F \*j QL53 \*k BicC \*f Schupbach \*l cn bw \# *F *F l(2)35Df *F *F 4 old, 1 new: *F *F \*j P15 \*k l(2)35Df \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br44 \# *F \*j P32 \*k l(2)35Df \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br44 \# *F \*j P36 \*k l(2)35Df \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br44 \# *F \*j RX1 \*k l(2)35Df \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br44 \# *F \*j PW144/23 \*b 35D5-7 \*d PlacW \*f Kiss, Szeged \*k l(2)35Df \*l wild-type \*m *F l(2)br44 \# *F *F l(2)35Dg *F *F 5 alleles only! *F *F \*j P29 \*k l(2)35Dg \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br45 \# *F \*j P31 \*k l(2)35Dg \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br45 \# *F \*j P34 \*k l(2)35Dg \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br34 \# *F \*j P40 \*k l(2)35Dg \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br45 \# *F \*j RAR77 \*k l(2)35Dg \*l b pr cn bw \*f Roth \*e EMS \*m l(2)br45 \# *F *F fzy *F *F 2 alleles: *F *F \*j X4 \*k fzy \*f Roth \*l b pr cn wx bw \*e EMS \# *F \*j IB115 \*k fzy \*l cn bw sp \*f Simpson \# *F *F sna *F *F 22 alleles: *F *F \*j 4.26 \*k sna \*e EMS \*l b pr cn wxt bw \*f Nusslein-Volhard \*m l(2)br28, *F l(2)35Db \# *F \*j 18.19 \*k sna \*e EMS \*l b pr cn wxt bw \*f Nusslein-Volhard \*m l(2)br28, *F l(2)35Db \# *F \*j IIGO5 \*k sna \*e EMS \*l cn bw sp \*f Nusslein-Volhard \*m l(2)br28, l(2)35Db \# *F \*j EY1 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \#. *F \*j EY2 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j EY3 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j HG31 \*k sna \*e EMS \*l Adh[n7]cn (vg) \*m l(2)br28, l(2)35Db \# *F \*j R1 \*k sna \*e X-rays \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j RY1 \*k sna \*e X-rays \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V1 \*k sna \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V2 \*k sna \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V3 \*k sna \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V4 \*k sna \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V5 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V6 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j V7 \*k sna \*e EMS \*l cn bw sp \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j VD1 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*j VD2 \*k sna \*e EMS \*l b pr cn bw \*f Pat Simpson \*m l(2)br28, l(2)35Db \# *F \*k sna \*j RY2 \*l b pr cn bw \*f Pat Simpson \*e X-rays \*o T(2;3)sna[RY2] \*m *F l(2)br28, l(2)35Db \# *F \*k sna \*j RY3 \*l b pr cn bw \*f Pat Simpson \*e X-rays \*m l(2)br28, l(2)35Db \# *F \*j ry40 \*k sna \*l b pr cn bw \*f Simpson \*m l(2)br28, l(2)35Db \# *F \*j AM15 \*k sna \*l GR28[2D] osp90[2P] \*m l(2)br28, l(2)35Db \# # *U FBrf0079820 *a Fogarty *b M. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079821 *a Matunis *b E. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079822 *a Tran *b J. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079823 *a Hartmann *b C. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079824 *a Murphy *b A. *t 1995.5.2 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue May 2 10:27:01 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 2 May 95 10:26:57 BST *F To: ammurphy@welchlink.welch.jhu.edu *F Subject: Curation of 36th Ann. Dros. Conf. abstract 123A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1294 *F Dear Dr. Murphy, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 123A, discusses a new gene rho-like. In your abstract *F you assign the symbol rol for this gene. Unfortunately this symbol has *F already been used for the gene reduced optic lobes. Therefore you *F shall have to decide upon a new symbol, please could you consult the *F database before deciding upon another symbol. *F In addition do you know the cytological or genetic location of the gene or *F the function of the gene product? *F As this is a new gene the information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F I apologise for having to bring this to your attention. *F Many thanks, *F Eleanor Whitfield. *F From ammurphy@welchlink.welch.jhu.edu Tue May 2 19:27:29 1995 *F Date: Tue, 2 May 1995 14:22:42 -0400 (EDT) *F From: ANNE MARIE MURPHY *F To: Eleanor Whitfield *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 123A *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 524 *F Dear Dr. Whitfield, *F actually subsequent to the receipt of abstracts and prior to the actual *F meeting, we had realised that rol was already assigned and therefore had *F consulted with other ras,rho,rac workers about assigning rhoL to our new *F member of the rho family of small GTPases. RhoL(pronounced roll) is we *F believe at 67A. Though this needs to be confirmed. The data is being *F written up with regard to its mutant phenotypes and homology to other rho *F family members. *F Thank you for your attention, *F Anne Marie Murphy # *U FBrf0079825 *a Eldon *b B. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079826 *a Cripps *b R. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079827 *a Konsolaki *b M. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079828 *a Kambadur *b R. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079829 *a Vincent *b A. *t 1995 *T personal communication to FlyBase *u *F Archived. # *U FBrf0079830 *a Hales *b K. *t 1995.5.4 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed May 3 08:55:10 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 3 May 95 08:54:59 BST *F To: karenh@leland.stanford.edu *F Subject: Curation of 36th Ann. Dros. Conf. abstract 137A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 957 *F Dear Dr. Hales, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 137A, discusses a new gene fuzzy onions. *F In your abstract you do not assign a symbol for the gene - have you *F decided upon the symbol yet? Do you know the function of the gene *F product? As this is a new gene this information will be very useful to *F users of FlyBase. *F Any information you can give me will be greatly appreciated. *F Many thanks, *F Eleanor Whitfield. *F From karenh@leland.stanford.edu Thu May 4 00:04:56 1995 *F Date: Wed, 3 May 1995 16:02:22 -0700 *F From: Karen Gwen Hales *F To: eleanor@gen.cam.ac.uk *F Subject: abstract 137A *F Content-Length: 242 *F I have been using fzo as the symbol for the fuzzy onions gene. The gene is *F not yet cloned (but will be soon); the phenotype is as described in the *F abstract. Please let me know if you need further information. *F Sincerely, Karen Hales # *U FBrf0079831 *a Frasch *b M. *t 1995.3.24 *T personal communication to FlyBase *u *F From FRASCH@msvax.mssm.edu Fri Mar 24 17:42:49 1995 *F Date: Fri, 24 Mar 1995 12:38:49 -0500 (EST) *F From: FRASCH@msvax.mssm.edu *F Subject: fly base 93E entries *F To: ma11@gen.cam.ac.uk *F X-Vms-To: IN%'ma11@gen.cam.ac.uk' 'ma11' *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 2830 *F *F Hi Michael, *F I've looked through the relevant fly base entries at 93E and here is some *F information and corrections: *F 1) C15: *F C15 is identical to 93Bal and 311. The reference for 311 is: Dear, T.N. and *F Rabbitts, T.H.: A Drosophila melanogaster homologue of the T-cell oncogene *F Hox11 localises to a cluster of homeobox genes. Gene 141, 225-229 (1994). *F Note that the speculations in these paper on the mesodermal function of *F this gene are wrong. Our in situ and antibody stainings show that it is *F expressed in amnioserosa cells, from cellularized blastoderm until the *F amnioserosa degenerates in late embryogenesis. From stage 11 on, there is *F also expression in small, segmentally repeated clusters of neurons of the *F CNS and in sensory cells of the Keilin's organs and of the antenno-maxillary *F complex. The mRNA is 2.0 kb and is mainly expressed in 8-24h old *F embryos and in pupae. The gene maps ~45 kb distally to S59/NK1. *F *F 2) tinman: *F Please add our paper to the references: Azpiazu, N. and Frasch, M.: tinman *F and bagpipe: two homeo box genes that determine cell fates in the dorsal *F mesoderm of Drosophila. Genes Dev. 7, 1325-1340 (1993). *F *F 3) prd9 *F This is identical to S59/NK1 *F *F 4) bagpipe: *F First a complaint: The gene symbol for bagpipe in the fly base was changed *F into bgp, whereas in our original paper, and meanwhile in several others, it *F was named bap. To avoid confusions, I think fly base shouldn't make these *F arbitrary decisions without consulting the authors. The gene that has been *F dubbed bap (beta adaptin) by fly base was published a year after bagpipe, *F and to my knowledge, no three-letter abbreviation was used in that paper. *F Therefore, I feel strongly that bagpipe should be renamed into bap in the *F flybase entry, and that fly base should accept the names as they appear in *F the first publication, unless there is a compelling reason for a change, *F such as duplications etc. *F Phenotypic information: *F During stages 10-11, bap is expressed in 11 segmentally repeated patches *F of dorsolateral mesodermal cells which are the primordial cells of the *F midgut visceral mesoderm. From stage 11 onwards it is also expressed in *F the foregut and hindgut visceral mesoderm and in some heart precursors, *F and the expression in the midgut visceral mesoderm disappears. The EMS *F allele bap[208] causes a severe reduction in the formation of the visceral *F musculature of the midgut. Musculature of the foregut, hindgut, and the *F heart appear normal. *F Molecular biology data: *F The protein is 382 amino acids *F (All these data are from Azpiazu and Frasch, 1993) *F Reference FBrf0051845: Delete this entry, because these data are about *F C15. *F Reference FBrf0050865: delete the possible identity with prd9 (see above) *F *F 5) NK2: *F I have no information on NK2 so please delete 'Frasch, personal *F communication'. *F *F Best regards, *F Manfred # *U FBrf0079832 *a McKee *b B. *t 1995.6.8 *T personal communication to FlyBase *u *F From MCKEE%UTK_BIOSCI%UTCC1%HUB%UTKVX@wpgate.utk.edu Thu Jun 8 20:39:12 1995 *F Date: Thu, 08 Jun 1995 15:35:32 -0500 (EST) *F From: Bruce D McKee *F Subject: Rad51 sequence *F To: m.ashburner@gen.cam.ac.uk *F X-Vms-To: WPGATE::IN%'m.ashburner@gen.cam.ac.uk' *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 409 *F *F Dear Michael, *F *F Sorry for the delay. For now it should be *F called Rad51. It is located in 99D3 6. There are no known *F repair or recombination genes there, but there are several *F essential complementation groups, which we are in the act of *F checking. I have not seen the Akaboshi et al. records but I *F knew of the paper. No question it's the same gene. Thanks. *F *F Yours truly, *F *F Bruce McKee # *U FBrf0080527 *a Roote *b J. *t 1995.5.15 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon May 15 12:06:10 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 15 May 1995 12:02:50 +0100 *F To: eleanor@gen.cam.ac.uk *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: PZ *F Content-Length: 1026 *F Eleanor, *F I made the mistake, some months ago, of renaming the PZ's that mapped to *F the Adh region. Some of these may appear in the literature. The correlation *F is: *F l(2)35Fd[PZ1] in-situ: 35F1-2 synonym(s): 00232 *F l(2)35Fd[PZ2] in-situ: 35F1-2 synonym(s): 03101 *F l(2)35Fd[PZ3] in-situ: 35F1-2 synonym(s): 06872 *F l(2)35Dd[PZ5] in-situ: 35D3-5 synonym(s): 05206 (e.g. appears in *F Knoblich et al Cell 77:107) *F l(2)35Dd[PZ6] in-situ: 35D3-5 synonym(s): 05277 *F l(2)35Dd[PZ7] in-situ: 35D3-5 synonym(s): 05278 *F l(2)35Dd[PZ8] in-situ: 35D3-5 synonym(s): 10427 *F l(2)35Cb[PZ9] in-situ: 35C1-2 synonym(s): 05441 *F l(2)esg[PZ18] &tgr;in-situ: 35D1-2 synonym(s): 05730 *F l(2)esg[P3] in-situ: 35D1-2 synonym(s): 05729, PZ10 (P3 was named by *F Shirras in Hayashi et al Dev 118:105) *F l(2)esg[PZ11] in-situ: 35D1-2 synonym(s): 07082 *F wb[PZ12] in-situ: 35A1-2 synonym(s): 09437 *F wb[PZ13] in-situ: 35A1-2 synonym(s): 10002 *F l(2)35Bd[PZ14] in-situ: 35B8-9 synonym(s): 10408 *F l(2)35Cd[PZ17] in-situ: 35D1-4 synonym(s): 06430 *F J # *U FBrf0080550 *a Hilfiker *b A. *t 1995.5.26 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Fri May 26 10:40:34 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 26 May 95 10:40:31 BST *F To: ahilfik@bimcore.emory.edu *F Subject: Curation of 36th Ann. Dros. Conf. abstract 223B *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 933 *F Dear Dr. Hilfiker, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 223B, discusses a new gene found at 1-13.8. *F Do you have a gene symbol or name for this gene and a cytological *F location? *F As this is a new gene this information is essential for *F curation purposes and will be very useful to users of FlyBase. *F Any information you can give me will be greatly appreciated. *F Many thanks, *F Eleanor Whitfield. *F From ahilfik@emory.edu Fri May 26 15:54:26 1995 *F X-Sender: ahilfik@dooley.cc.emory.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 26 May 1995 10:58:17 -0500 *F To: Eleanor Whitfield (Genetics) *F From: ahilfik@emory.edu (Andres Hilfiker) *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 223B *F Content-Length: 1629 *F Dear Eleanor, *F as given on our poster, our mutation, resp. gene is named : max = *F males-absent on the X *F The cytological location is not clear yet. *F Sincerely, *F Res # *U FBrf0080551 *a Leung *b R. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080552 *a Certel *b K. *t 1995.6.5 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue May 30 10:19:01 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 30 May 95 10:18:56 BST *F To: kcertel@blue.weeg.uiowa.edu *F Subject: Curation of 36th Ann. Dros. Conf. abstract 327A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1020 *F Dear Dr. Certel, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 327A, discusses a new gene required for the formation of *F the embryonic ventral nerve cord. *F In the abstract you do not give a gene symbol or name for the gene, *F could you please tell me what you have decided upon. *F As this is a new gene this information is vital for curation purposes *F and will be very useful to users of FlyBase. *F Any information you can give me will be greatly appreciated. *F Many thanks, *F Eleanor Whitfield. *F From kcertel@blue.weeg.uiowa.edu Mon Jun 5 17:11:59 1995 *F Date: Mon, 5 Jun 1995 11:04:50 -0500 (CDT) *F From: kaan certel *F X-Sender: kcertel@red.weeg.uiowa.edu *F To: Eleanor Whitfield *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 327A *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 872 *F Dear Ms. Whitfield, *F We have named the locus presented in the abstract 327A this past fly *F meeting as 'ripcord' with the acronym 'rip'. We checked the flybase to *F make sure there were no other genes with the same name or acronym at the *F time. I am sure you have better records to double check this. *F 'ripcord' maps to cytological position 65D1-4 as revealed by a small *F deficiency that was generated in our lab. As we mentioned in our *F abstract, we have both X-ray- and EMS-induced alleles of ripcord. Two of *F these alleles are temperature sensitive. *F I hope this information will be useful. If there is anything else you *F would like to know please do not hesitate to contact me by e-mail, or by *F phone. My apologies for replying so late. I did not get a chance to check *F my e-mail messages since I was on a short vacation. *F Sincerely, *F Kaan. *F From eleanor@gen.cam.ac.uk Tue Jun 6 08:13:57 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 6 Jun 95 08:13:53 BST *F To: kcertel@blue.weeg.uiowa.edu *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 327A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 481 *F Dear Kaan, *F Many thanks for your reply. *F Thank you for checking your gene symbol against present symbols in *F FlyBase, I have double checked this and you are right that there are no *F other genes with this symbol. *F As you have localised rip using deficiency mapping, as opposed to in *F situ hybridisation, could you please give me the name of the *F deficiency. The curation methods only allow me to trap this *F information under the deficiency itself, not under the gene. *F Eleanor Whitfield. *F From kcertel@blue.weeg.uiowa.edu Tue Jun 6 23:05:03 1995 *F Date: Tue, 6 Jun 1995 17:00:21 -0500 (CDT) *F From: kaan certel *F X-Sender: kcertel@red.weeg.uiowa.edu *F To: Eleanor Whitfield *F Subject: Re: Curation of 36th Ann. Dros. Conf. abstract 327A *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 527 *F Dear Ms. Whitfield, *F The name of the deficiency you requested is Df(3L)XBB70. By looking at *F the polytene squashes of this deficiency, we see the loss of a single *F band in the cytological region 65D1-3. For more information please see *F the following reference: *F Andersen et al. 1995. drifter, a Drosophila POU-domain transcription *F factor, is required for correct differentiation and migration of tracheal *F cells and midline glia. Genes & Dev. 9:123-137. *F Hope this will help. Please let me know if you need anything else. *F Kaan. # *U FBrf0080557 *a Konev *b A. *t 1995.6.8 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Apr 13 12:29:58 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 13 Apr 95 12:29:51 BST *F To: Korolev@lnpi.spb.su *F Subject: Curation of 36th Ann. Dros. Conf. abstract 145A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1486 *F Dear Dr. Konev, *F I am a curator of FlyBase at the Cambridge, England, site working with *F Prof. M. Ashburner. *F I shall be curating the abstracts from the 36th Ann. Dros. Res. Conf. *F Your abstract, 145A, discusses a new gene gorbun. In your abstract you *F assign the symbol gor for this gene. Unfortunately this symbol has *F already been used for the gene gorp. Therefore you shall have to *F decide upon a new symbol. I have looked at the database and have some *F suggestions for a new symbol: grb, gob and gon are vacant, but grn is *F not. Of course the choice is yours, but please consult the database *F before deciding upon another symbol. *F In addition do you know the cytological or genetic location of the gene or *F the function of the gene product? *F As this is a new gene the information will be very useful to users of *F FlyBase. *F Any information you can give me will be greatly appreciated and will be *F curated as a personal communication. *F I apologise for having to bring this to your attention, I tried to find *F you at the Conference but unfortunately failed! *F Many thanks, *F Eleanor Whitfield. *F From korolevbgr;iod.lnpi.spb.subgr;iod.uucplgr;npi.uucp@rcom.spb.su Thu Jun 8 14:20:34 1995 *F To: eleanor@gen.cam.ac.uk *F Organization: St. Petersburg Nuclear Physics Institute, Russia *F From: Vladimir G. Korolev *F Date: Thu, 8 Jun 95 16:43:03 +0300 *F X-Mailer: BML [MS/DOS Beauty Mail v.1.36] *F Lines: 15 *F Content-Length: 834 *F Dear Mrs. E.J. Whitfield, *F I am very sorry that I am answering only on your letter *F which I have received just recently. At April 7 - May 4 I was in *F the USA and I had not received your E-mail. *F Thank you very much for your information concerning the *F symbol for gorbun gene. I have decided grb as a new symbol for *F the gene. Unfortunately I have not still of direct access to Internet *F network and it is not very easy for me to consult the FlyBase. I *F would be very grateful to you if you could let me know if this *F new choice is correct or not. *F I have mapped the grb at the 45A13-B2. There is not still *F the information about the sequence of the gene. The grb *F phenotype is described in my abstract. *F Sincerely yours, *F Alexander Konev. # *U FBrf0080566 *a Byars *b C.L. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080567 *a Guillemin *b K. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080568 *a Kidd *b T. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080569 *a Semeriva *b M. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080570 *a Kurapati *b R. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080571 *a Petscheck *b J. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080572 *a Sasamura *b T. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080573 *a McKay *b J. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080574 *a Warrick *b J. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080575 *a Simmonds *b A. *t 1995.5.30 *T personal communication to FlyBase *u *F From asimmond@gpu.srv.ualberta.ca Tue May 30 16:37:04 1995 *F Date: Tue, 30 May 1995 09:30:35 -0600 (MDT) *F From: Andrew Simmonds *F X-Sender: asimmond@gpu2.srv.ualberta.ca *F To: rd120 *F Subject: Re: helping flybase *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 494 *F vg[WRK2] (which has been subsequently re-named inv[D]), is a derivative *F allele of vg[W]. vg[W] is caused by an inversion fusing the vg and inv *F alleles, (see Williams et al, 1990). The inv[D] allele was created by *F subjecting vg[W] to gamma rays. Removing the 3' end of the vg portion of *F the fused genes in vg[W] gives the inv[D], (vg[WRK2]) phenotype. *F All this data has been submitted for publication and I will send you the *F ref, as soon as it is accepted somewhere. *F Andrew Simmonds # *U FBrf0080576 *a Maroteaux *b L. *t 1995.5.31 *T personal communication to FlyBase *u *F From lucm@titus.u-strasbg.fr Wed May 31 10:08:52 1995 *F From: lucm@titus.u-strasbg.fr *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-Mailer: Eudora F1.4.3 *F Date: Wed, 31 May 1995 11:15:54 +0100 *F To: rd120@gen.cam.ac.uk *F Subject: serotonin receptor *F Content-Length: 1210 *F To Rachel Drysdale *F FLyBase *F Concerning the Atlanta 's poster about a new 5-HT2 Drosophila receptor, the *F 5-HT2Dro cDNA and genomic sequences have been deposited at the EMBL/Gene *F Bank under the accession numbers X81835 and X85407 respectively. This *F 5-HT2Dro is a newly (the fourth) cloned Drosophila serotonin receptor gene *F located in the third chromosome at 82C-82D. *F In contrast with the 3 previously published Drosophila 5-HT receptors which *F are of the 5-HT1 subtype, acting on the adenylyl cyclase, this 5-HT2Dro *F receptor displays a sequence, a gene organisation and a pharmacology *F typical of the mammalian serotonin 5-HT2 receptor type (5-HT2B subtype) *F acting on the PLC. *F So, in order to avoid confusion and in accordance with the new mammalian *F nomenclature, we propose (in a paper to be published in PNAS in June : *F Colas & al / Drosophila serotonin 5-HT2 receptor : co-expression with *F fushi-tarazu during segmentation, that I will send you by mail) that the *F 5-HTDro2A, 5-HTDro2B and 5-HT1Dro be renamed 5-HT1ADro, 5-HT1BDro and *F 5-HT7Dro respectively. *F Thanks *F from Luc Maroteaux *F IGBMC-CNRS-INSERM *F Strasbourg University *F BP 163 *F 67404 ILLKIRCH Cedex *F FRANCE *F Phone# 33 88 65 33 85 *F Fax # 33 88 65 32 01 # *U FBrf0080577 *a Roche *b N. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080578 *a Soanes *b K. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080579 *a Kennison *b J.A. *t 1995 *T personal communication to FlyBase *u *F Archived # *U FBrf0080580 *a Corbo *b J. *t 1995.6.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Jun 1 09:08:10 1995 *F From: Rachel Drysdale (Genetics) *F Date: Thu, 1 Jun 95 09:04:16 BST *F To: jcorbo@ucsd.edu *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 646 *F Dear Dr. Corbo, *F I am curating your abstract from the Atlanta Fly meeting (271B) and *F would like to include a reference to the immune response gene *F you describe. Do you have a name for this gene yet? *F Many thanks for your help, *F best wishes, *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Joseph_Corbo@som-lrc.ucsd.edu Sun Jun 4 03:03:04 1995 *F From: Joseph_Corbo@som-lrc.ucsd.edu *F Date: Sat, 3 Jun 1995 17:09:00 -0700 *F To: rd120@gen.cam.ac.uk *F Subject: Re: helping FlyBase *F Content-Length: 475 *F Rachel, *F I do not have a name for the immunity locus, but I believe Jules Hoffmann's *F group in France (working on the same locus) has called it Imd (for *F ImmunoDeficiency locus). Both the French group and ourselves (Mike Levine's lab) *F are submitting separate papers on the gene to PNAS so these should hopefully be *F out in the coming months. Please let me know if you need any more information, I *F realize my abstract was a bit vague. Sincerely in science, Joseph Corbo # *U FBrf0080581 *a Cohen *b S.M. *t 1995.6.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Jun 6 16:08:10 1995 *F From: Rachel Drysdale (Genetics) *F Date: Tue, 6 Jun 95 16:03:24 BST *F To: scohen@embl-heidelberg.de *F Subject: helping flybase *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 779 *F Hi Steve, *F In connection with a paper I am curating for FlyBase (Kaphingst and *F Kunes, Pattern formation in the visual centers... Cell 78:437) I am *F interested in finding out whether l(2)01092, a Ba/Dll enhancer-trap *F insertion, is actually a Ba/Dll allele. Sam Kunes tells me that you had *F planned to test this by complementation. Did you ever do this? *F Many thanks for your help. *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Stephen.Cohen@embl-heidelberg.de Tue Jun 6 16:13:29 1995 *F Date: Tue, 06 Jun 1995 17:13:11 +0100 *F From: Stephen Cohen *F Content-Transfer-Encoding: 7BIT *F To: rd120@gen.cam.ac.uk *F Subject: Re: helping flybase *F To: 'rd120' *F X-Mailer: VersaTerm Link v1.1.1 *F Content-Length: 154 *F Hi Rachel *F l(2) 01092 is allelic to Dll, and behaves as a moderate hypomorph, according *F to its phenotype in trans to other characterized alleles. *F Steve # *U FBrf0080582 *a Hultmark *b D. *t 1995.6.7 *T personal communication to FlyBase *u *F From dan@molbio.su.se Wed Jun 7 14:13:39 1995 *F From: dan@molbio.su.se *F Date: Wed, 7 Jun 1995 15:33:57 +0200 *F To: rd120 *F Subject: Re: helping FlyBase *F Content-Length: 658 *F Dear Rachel, you asked if we have a name yet for the NF-kappaB-like gene we *F described at the Atlanta Fly meeting (abstract 269A). Yes, we now call it *F Relish (Rel). *F Best wishes, *F Dan *F __________________________________________________ *F Dan Hultmark *F lab. *F Address: Department of Molecular Biology *F Stockholm University *F S-106 91 Stockholm, Sweden *F Telephone: +46-8-16 41 53 *F Fax: +46-8-15 23 50 *F E-mail: dan@molbio.su.se *F home *F Address: Fjallstigen 27 *F S-131 41 Nacka, Sweden *F Telephone: +46-8-718 08 67 *F __________________________________________________ # *U FBrf0080583 *a Tully *b T. *t 1995.6.8 *T personal communication to FlyBase *u *F From tully@cshl.org Thu Jun 8 17:25:14 1995 *F Date: Thu, 8 Jun 95 12:22:05 EDT *F X-Sender: tully@phage.cshl.org *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: rd120 *F From: tully@cshl.org *F Subject: Re: helping flybase *F Content-Length: 791 *F Hi Rachel, *F The Yin et al. (submitt.) manuscript now is in press in MCB. It contains *F the molecular details that relate one CREB family member to another. Jerry will Email you with the appropriate info. *F I might add that in perusing FlyBase, I noticed entries for latheo and *F linotte. Cytological location of the latter is incorrect (it should be *F 37D), and the molecular information -- derived from an abstract -- is *F demonstrably wrong. This week we will submit a manuscript describing the *F full rescue of the linotte learning defect with and inducible lio+ *F transgene. Importantly, this transcript is not the homologue of hRTK, but *F rather the NEIGHBORING transcript, which itself is novel. More on this *F later. *F later... *F \--- *F TT # *U FBrf0080584 *a Celniker *b S.E. *t 1994.9.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0080585 *a Kaufman *b T. *t 1994.10.5 *T personal communication to FlyBase *u *F Archived # *U FBrf0080586 *a Thomas *b B. *t 1995.6.15 *T personal communication to FlyBase *u *F Dear Dr. Thomas, *F I am curating your roughex paper for FlyBase (Thomas et al., 1994, *F Cell 77(7): 1003--1014). In recording your rux[6] allele I see that *F you have the cytology down as being a T(2;3), but as rux is on the X *F there must be a typo somewhere! Please could you let me know what the *F aberration really is (and if you have any more info on the cytology of *F rux[6] and rux[7] that would be nice too). *F Many thanks for your help, *F yours sincerely, *F Rachel Drysdale *F FlyBase (UK) *F From THOMAS@hhmi.ucla.edu Thu Jun 15 20:57:45 1995 *F To: rd120@gen.cam.ac.uk *F From: THOMAS@hhmi.ucla.edu *F Date: Thu, 15 Jun 1995 11:57:50 PST *F Subject: roughex paper *F Priority: normal *F X-Mailer: Pegasus Mail/Mac v2.0.5 *F Content-Length: 1041 *F Dear Rachel, *F I am writing concerning your fax of 1 June about the paper Thomas et. *F al. (1994) Cell 77: 1003. *F You are indeed correct about the cytology of the allele rux[6]. I have *F not done the cytology very well, but it appears to be a T(1;2). The *F last band I can definitively see on the X chromosome is 4C1,2. The *F break in 2R appears to be in 56D. *F Regarding rux[7], this allele is an inverted duplication of several *F bands from 5D back into the rux locus. Molecularly, one insertion *F breakpoint is in the rux gene, while the second insertion breakpoint is *F in an adjacent transcript that is next to rux, about 5 kb away. The *F insertion appears to be composed of the bands 5D3,6 (two doublets). I *F know that it is an inverted duplication because the insertion loopout *F is seen in hemizygous males. *F I hope that this is helpful. Please contact me if you require any *F additional information. *F Sincerely yours, *F Barbara Thomas *F From rd120@gen.cam.ac.uk Thu Jun 15 22:59:05 1995 *F From: Rachel Drysdale (Genetics) *F Date: Thu, 15 Jun 95 22:54:44 BST *F To: THOMAS@hhmi.ucla.edu *F Subject: Re: roughex paper *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 2816 *F Hi Barbara, *F Thank you for your email. In fact I do have a couple more questions, *F and I'm sure you are the right person to answer them! *F What you say about rux[6] suggests to me that it is a 2 break event, *F with one break between 4B6 and 4C1 and the other break in 56D. *F We have a statement in FlyBase that rux is *F 'Placed in 5C5-D6, possibly in 5D2-6 (Ashburner).' *F So *F either the X chromosomal break in rux[6] is not related to the T(1;2) *F or *F the T(1;2) has more than two breaks *F or *F we (and Lindsley and Zimm, where this statement came from) have the *F cytology of rux all wrong. *F Can you say which of these three is the answer? It matters because of *F the way we link aberrations to alleles. If the aberration causes the *F allele then the allele name becomes part of the aberration name, and we *F link allele and aberration in the database, but not if not. *F >Regarding rux[7], this allele is an inverted duplication of several *F >bands from 5D back into the rux locus. Molecularly, one insertion *F >breakpoint is in the rux gene, while the second insertion breakpoint *F >is in an adjacent transcript that is next to rux, about 5 kb away. *F >The insertion appears to be composed of the bands 5D3,6 (two *F >doublets). *F So, to check my understanding, are you saying that there is a deletion *F of (not much) more than 5kb, that breaks in the rux gene and the *F adjacent gene, and that the insertion of 5D3,6 occurs between the two *F deletion breaks? Can you say what the cytological location of rux is? *F You imply that its location is distinct from 5D3,6 when you say 'bands *F from 5D back into the rux locus'. *F I hope my questions aren't a nuisance. We like to get as much genetic *F data from the papers as possible, to do justice to the work. The info *F in this correspondance between us will be incorporated as a personal *F communication to FlyBase from you, and will allow me to curate your *F Cell paper more completely. *F Many thanks for your help *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From THOMAS@hhmi.ucla.edu Fri Jun 16 18:25:40 1995 *F To: rd120@gen.cam.ac.uk *F From: THOMAS@hhmi.ucla.edu *F Date: Fri, 16 Jun 1995 10:12:02 PST *F Subject: rux paper *F Priority: normal *F X-Mailer: Pegasus Mail/Mac v2.0.5 *F Content-Length: 3713 *F Dear Rachel, *F What you say about rux[6] is true, that the X chromosome breakpoint is *F nowhere near the cytological position of the gene. In fact, the locus *F is even more precisely localized by another deficiency that was *F generated by Roger Karess in his spaghetti squash paper, Df(1)sqh, *F which uncovers rux and goes from 5D1,2; 5E. This, as you've probably *F worked out, puts rux in the interval 5D1,2; 5D5,6. *F There is a caveat to the information that I gave you about these *F alleles: I am far from being an expert at doing and scoring chromosome *F squashes. However, I would be surprised if I were that far off in *F reading the bands. However, I did do some analysis of rux[6] at the *F molecular level, and the Southerns were consistent with there being a *F break in the rux gene. I guess the way that I would interpret the *F results is that was a fairly large deletion associated with the *F translocation that removed the DNA between 4C1 and 5D1,2; 5D5,6. I *F guess the other way to figure this out would be to look at the other *F translocation chromosome, which I have not done in detail. *F Regarding rux[7], most of what I know is from the molecular analysis - I *F have not looked very hard at squashes, except to determine that there *F was extra material in the 5D region and that it was probably an *F inverted duplication because it was present in males. What I know from *F the molecular analysis is that there indeed appear to be a deletion *F between the two breakpoints: this was based on looking at three *F adjacent genomic fragments (they are shown in the paper) of 3.3, 1.2, *F and 6.0 kb. The 1.2 kb band, which is in the middle, is missing, and *F one break is in the 3.3 kb fragment (which contains the adjacent cDNA) *F and one is in the 6.0 kb fragment (which contains rux). The rux gene *F resides closer in the 6 kb fragment to the 1.2 kb fragment than the *F other side, so I am estimating that the amount of DNA removed is *F approximately 5 kb, although it might be greater. *F The sequences actually contained within the duplication come from near, *F but not in, the rux locus. The rux genomic region is contained within *F two overlapping cosmids: the cosmids are each about 35 kb, and the *F region of overlap is about 25 kb. Rux lies almost precisely in the *F middle of this 25 kb overlap. In situs to the rux[7] chromosome with *F the cosmids shows that one cosmid hybridizes to a single band just *F distal to the insertion, while the other cosmid hybridizes both to this *F band and to the insertion itself. In addition, a probe from the most *F proximal end of this second cosmid also recognizes the insertion. This *F probe is about 20 kb away from the 6.0 kb fragment containing the rux *F gene. Based on these hybridization patterns, I am assuming that the *F DNA in the insertion comes from proximal to the rux locus, and the *F bands in the insertion look like they could be the 5D3,4 and 5D5,6 *F doublets. *F I hope that this all makes sense. *F With best regards, *F Barbara Thomas # *U FBrf0080587 *a Rabinow *b L. *t 1995.5.24 *T personal communication to FlyBase *u *F From nobody@morgan.harvard.edu Wed May 24 21:23:07 1995 *F Date: Wed, 24 May 95 16:23:23 EDT *F From: nobody@morgan.harvard.edu *F To: flybase-updates@morgan.harvard.edu *F Content-Length: 796 *F Date: 5-24-95 *F Reply-to: rabinow@mbcl.rutgers.edu (Leonard Rabinow) *F Sender's Address: Waksman Inst/Rutgers *F Sender's Phone : (908) 445-0092 *F Sender's FAX : (908) 445-5735 *F Subject: FlyBase-updates mail *F rabinow@mbcl.rutgers.edu (Leonard Rabinow) sent the following *F updates to flybase-updates: *F \------------------------------------------------------------ *F l(3)01705 from the Spradling recessive lethal *F P-element collection, mapped and indexed in the EofD, *F is an allele of Doa, based on the following observations: *F 1) Failure of l(3)01705 to complement recessive lethality *F of some, but not all Doa alleles. *F 2) Suppression of white-apricot. *F \------------------------------------------------------------ *F Server protocol: HTTP/1.0 *F Remote host: tyranosaurus.rutgers.edu *F Remote IP address: 128.6.11.14 # *U FBrf0080711 *a Saito *b H. *t 1995.6.23 *T personal communication to FlyBase *u *F From haruo_saito@macmailgw.dfci.harvard.edu Fri Jun 23 20:19:57 1995 *F Received: from netop3.harvard.edu by hmg1.gen.cam.ac.uk (4.1/MDTG-V1.1@gen.cam.ac.uk) *F id AA09745; Fri, 23 Jun 95 20:19:53 BST *F Return-Path: *F Received: by netop3.harvard.edu (5.65/DEC-Ultrix/4.3) *F id AA13552; Fri, 23 Jun 1995 15:13:27 -0400 *F Received: from farber.harvard.edu by morgan.harvard.edu (4.1/SMI-4.1) *F id AA19434; Fri, 23 Jun 95 15:16:26 EDT *F Received: from MACIS_49.dfci.harvard.edu by farber.harvard.edu (5.65/1.34) *F id AA16916; Fri, 23 Jun 95 15:10:49 -0400 *F Message-Id: *F Date: 23 Jun 1995 15:09:03 -0500 *F From: 'Haruo Saito' *F Subject: DPTP etc. *F To: 'FlyBase Update' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 1795 *F X-Lines: 38 *F Status: RO *F *F Subject: Time: 1:14 PM *F OFFICE MEMO DPTP etc. Date: 6/23/95 *F *F Dear colleagues at Flybase, *F *F Upon your request (and I always wanted to do something about this any way), I *F checked flybase entries related to PTPase genes. Followings are short summary *F of my finding and recommendation. *F *F FlyBase entry 'Dlar' is mostly correct, except that the cytological location *F of the gene should be 38A1. It might be useful to add that the screw gene *F (scw) is located within the Dlar gene (within one of the Dlar intron), which *F is mentioned in reference FBrf0076510 by Arora, Levine, and O'Conner. *F *F FlyBase entry 'Ptp61F' has many problems. I understand that you do not like *F the prefix D for Drosophila genes, so calling this gene Ptp61F must be OK. *F In Function(s) of product, it should be 'cytosolic and nuclear protein *F tyrosine phosphatase', and delete the current description. *F In Synonym(s), you should only keep dPTP61F (i.e., delete DPTP, Pptp, and *F Ptp). *F In DNA/RNA AC no(s), M27699 must be removed. *F In Protein AC no(s), both PIR/B36182 and SWP/P16620 should be removed. *F In Reference, the first reference (FBrfOO50872) should be entirely removed. *F FBrf0058795 is correct. *F In gene Reference(s), FBrf0050872 should be removed. *F *F Then there will be several other DPTP entries (DPTP4E, 10D, 64D [= original *F DPTP], and 99A) that must be added. For 10D and 99A, please contact Kai *F Zinn at Caltech (Phone 818-395-8352; e-Mail zinnk@starbase1.caltech.edu). For *F 4E, contact William Chia at National University of Singapore (e-Mail *F mcbwchia@leonis.nus.sg). I will supply the information about 64D. Is there *F any on-line (or off-line) format that I can fill-in ? *F *F I added our names to FlyBase People today. *F *F Haruo Saito *F *F *F *F >From haruo_saito@macmailgw.dfci.harvard.edu Fri Jun 23 21:54:45 1995 *F Date: 23 Jun 1995 16:42:22 -0500 *F From: 'Haruo Saito' *F Subject: PTP69D *F To: 'FlyBase Project Members' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 2894 *F *F Reply to: PTP69D *F *F Dear Beverley, *F *F I missed 4E, 10D, etc, because I am used to think of them as DPTP rather than *F PTP, but obviously species designation is meaningless within the Drosophila *F data base. In any case, followings are the relevant information concerning *F PTP69D. I will follow the format as shown in the database. *F *F Gene symbol: Ptp69D. *F Synonym: DPTP, DPTP69D. *F Full name: protein tyrosine phosphatase 69D. *F Cytological map position: 69D by in situ hybridization. *F Function(s) of product: Immunoglobulin and fibronectin-type-III-repeat *F containing protein. *F Enzyme name/number: protein-tyrosine-phosphatase == EC 3.1.3.48. *F DNA AC no: M27699 (Genbank/EMBL). *F Protein AC no(s): PIR/B36182 and SWP/P16620. *F Reference FBrf0050872 reports the following: *F cDNA clone length: 4.8 *F Open reading frame size: 1462 aa *F Phenotypic information: PTP69D is a member of a family of receptor linked *F protein tyrosine phosphatases. *F *F Gene Reference(s): FBrf0050872. *F *F *F Thank you very much for your effort. *F *F *F *F -------------------------------------- *F Date: 6/23/95 3:58 PM *F To: Haruo Saito *F From: FlyBase Project Members *F Dear Dr. Saito, *F *F Thank you very much for your prompt response to my query. We will *F incorporate your communication in a future update. *F *F > Then there will be several other DPTP entries (DPTP4E, 10D, 64D [= original *F > DPTP], and 99A that must be added. *F *F Actually there are gene entries in FlyBase for Ptp10D (FBgn0004370), *F Ptp4E (FBgn0004368), and Ptp99A (FBgn0010251). I'm not sure how you are *F accessing FlyBase data but a very easy way to get to these is to use *F the FlyBase WWW page (http://morgan.harvard.edu). Select FlyBase Data *F Access and then choose the SymbolSearch option. If you search for Ptp*, *F you will get all the genes I have mentioned. If you choose the genes *F option from the data access page you can search for tyrosine *F phosphatase to get a more extensive list of Drosophila genes encoding *F tyrosine phosphatases. Thanks in advance for any more corrections you *F may have. *F *F > I will supply the information about 64D. Is there *F > any on-line (or off-line) format that I can fill-in ? *F *F There is no form to fill out. Just send another note to *F flybase-updates@morgan.harvard.edu. Thanks again. We really appreciate *F your help. *F *F Beverley Matthews for FlyBase # *U FBrf0080712 *a Kaufman *b T.C. *t 1995.6.28 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Mon Jun 26 21:36:03 1995 *F *F Dp(3;3)Antp1: groan. We have no Tp(3;3)Antp1. We do have in abs: *F *F - In(3R)Antp[rv1P] from L&Z, which looks like the same mutation in view *F of the footnote about the material being Tp'd. *F - In(3R)Antp1P which was called Df(3R)Antp1P in L&Z and looks like our *F required segregant *F - A separate Df(3R)Antp1P (synonym RIP -- I wish) curated from three *F papers, which doesn't mention any inversion but looks suspicious *F *F Putting all this together, miraculously there is only one interpretation *F that's moderately consistent with everything (even almost the cyto), viz: *F *F original was 4-break: 84B1-84B2 (or 84B3);84D1-84D4;85C2-85C3 (or 84D);87B *F new order 61 - 84B1 | 85C2 - 84D4 | 85C3 - 87B | (84B2 - 84D1) | 87B - 100 *F separeated by recombination around region 86 *F *F Is this right? If so then TK do you know the cyto any better, eg which *F way round the insertion is; also should that original chromosome be *F called a Tp or an In ?? (Either way the segregants will be called Df *F and Dp.) Also should the suffix be 1, 1P, rv1P, or some other rune? If *F you can tell us which Antp allele was the progenitor for this, In(3R) *F rv2 and T(2;3) rvA1 (Abbott 1986) we would be even happier! *F *F Cheers, Aubrey *F *F From kaufman@sunflower.bio.indiana.edu Wed Jun 28 21:08:59 1995 *F Mime-Version: 1.0 *F Date: Wed, 28 Jun 1995 15:14:38 -0500 *F To: ag24 *F From: kaufman@sunflower.bio.indiana.edu (T. Kaufman) *F Subject: Re: outstanding ab things *F Content-Length: 2380 *F *F Aubrey: *F The following is my reconstruction of the Antp1 business: *F *F The parent chromosome was Antp[73b]. This was a spontaneous allele of Antp *F found by Mel Green. It is associated with an inversion *F In(3R)84B1,2-3;84C5. *F *F New order 81-84B1,2|84C5-84B3|84C6-100 *F *F Mel shortly after finding this mutant also found two apparent spontaneous *F revertants of the original dominant and sent all three stocks here where *F the cytology was done. One of these is relevant to your query and we *F called it Antp[73b+R1] using terminaology suggested by Dan Lindsley at the *F time. Cytological analysis revealed that the parent inversion was still *F there but that now a bit of 84CD had been transposed to 87B. The cytology *F should read (I guess) In(3R)84B1,2-3;84D1,2-3,4 + *F Tp(3;3)84C6;84D1,2-3,4=>87B. It is very difficult to say whether any of *F the originally inverted material is involved in the transposed fragment. *F The genetics argue that not much if any is. *F *F New order 81-84B1,2|84C5-84B3|84D5-87B||84C6-84D3,4||87B-100 *F *F *F I then took this chromosome and recombined it with a marked normal sequence *F homologue to separate the transposed material from the inversion. The two *F products were called Antp[73b+R1P] and Antp[73b+R1D]. The P and D in the *F supers stand for Proximal and Distal respectively. The Antp[73b+R1P] *F chromosome carries the inversion and deletion while the Antp[73b+R1D] *F chromosome has the transposed material in 87B and a normal sequence in 84 *F and is thus duplicated for that interval. Thus Antp[73b+R1P] = *F In(3R)84B1,2-3;84D1,2-3,4 + Df(3R)84C3,4;84D3,4 and Antp[73b+R1D] = *F Dp(3;3)84C3,4-84D3,4 *F *F New order R1P 81-84B1,2|84C5-84B3|84D5-100 *F New order R1D 81-87B||84C6-84D3,4||87B-100 *F *F Thom Kaufman kaufman@sunflower.bio.indiana.edu *F --- Biology Dept., Indiana University, Bloomington, IN 47405 --- *F 812-855-3033/Office--812-855-7674/Lab--812-855-2577/FAX # *U FBrf0080713 *a Kaufman *b T.C. *t 1995.6.29 *T personal communication to FlyBase *u *F From kaufman@sunflower.bio.indiana.edu Wed Jun 28 23:08:13 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 28 Jun 1995 17:14:11 -0500 *F To: ag24 *F From: kaufman@sunflower.bio.indiana.edu (T. Kaufman) *F Subject: Re: another quick one *F Content-Length: 1241 *F *F >while implementing that one I discovered two In(3R)s which looks odd *F > *F >In(3R)Antp[a74] *F >In(3R)Antp[rA74] *F > *F >They are both in L&Z, p960 and 961. Are they REALLY different? The *F >cyto certainly is but if so then an absurder naming scheme I have *F >never seen, and I've seen a few. *F *F As far as I can tell these are the same. Antp[a74] was made by Mike Abbott *F and is associated with In(3R)84B2;87C. The [a74] allele designation is *F what we first called it. Dan then pointed out that Antp is a dominant and *F that the traditional nomenclature should now include an 'r' in the super to *F indicate that this allele is 'recessive' and not Dominant. Dan also *F requested that we renumber the recessive mutants so you will see that on *F page 33 there is a table that lists Antp[a74] as Antp[5]. I did not write *F the ab description material on pages 960-961. It seems clear to me however *F that the In(3R)Antp[r-A74] entry is in error and should be deleted. The *F In(3R)Antp[a74] entry is correct and matches the description on page 33 and *F what I know [a74] to be. --tk *F *F Thom Kaufman kaufman@sunflower.bio.indiana.edu *F --- Biology Dept., Indiana University, Bloomington, IN 47405 --- *F 812-855-3033/Office--812-855-7674/Lab--812-855-2577/FAX # *U FBrf0080715 *a Wasserman *b S. *t 1991.4 *T personal communication to FlyBase *u *F Letter from Sherry Alexander in Steve Wasserman's lab. to Michael Ashburner, *F together with stocks. April 1991. *F Copy available from FlyBase. # *U FBrf0080716 *a Engels *b W.R. *t 1995.6.27 *T personal communication to FlyBase *u *F Personal communication from: Bill Engels *F To: FlyBase *F Dated: 27 Jun 1995 *F Background: Bill was asked if had any information to provide for a new 'wild *F stocks' sections of FlyBase. *F Information communicated: *F ---------------- Pi[[2]] *F *F First described in FBrf0033167. *F *F Taken from a Madison wild population in 1976 and inbred by pair matings for *F many generations. It was selected from several such inbred lines for the *F high level of GD sterility it caused. *F *F P element sites on the X include 2F, 5E, 11A, 17C (FBrf0035947, *F FBrf0040973, FBrf0056156) *F *F Contains a mixture of autonomous and nonautonomous P elements *F (FBrf0041720, FBrf0038996) *F *F ----------------- Birmingham *F *F This is not really a wildtype strain since it has M5. It has many P elements, *F but no autonomous ones. *F *F P element sites include: *F X: 1C, 1D, 1F, 2D, 3B, 3E, 5D, 6D, 7B, 7D, 9E, 12F, 13C, 13E, 16A, 18C, *F 18D, 18F, 19A, 19C *F 2L: 21B, 23B, 25E, 28C, 28D, 30C, 38C, 39C *F 2R: 41B, 44B, 47A2-5, 47A13-16, 47C, 48F, 52D, 55B, 55C, 56E, 57A, 60BC *F 3L: 61E, 62A, 62D, 64DE, 67C, 69A, 73F, 75F, 76C, 78D *F 3R: 84E, 86E, 87E, 88B, 89A, 89C, 90E, 91A, 97A, 97EF, 100D *F 4: No sites *F Heterochromatin: No sites *F *F The origin of the P elements in this line is not known, and other M5 lines *F have no P elements. However, a good guess is that it was contaminated while *F in the Mather lab in Birmingham, England. # *U FBrf0080717 *a Collier *b S. *t 1995.7.4 *T personal communication to FlyBase *u *F Michael - I have checked the cytology and genetic data against Claire *F Henchcliffes notes in our flylab. All are correct for these records. *F *F Simon Collier *F 4 July 1995 *F *F \*a Df(2L)TE29Aa-15 *F \*z FBab0001571 *F \*H Not updated after 20 May 94 *F \*x Ashburner, unpubl. *F \*x FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*B 29A2-29B1;29F2-29F6 *F \*C Deficiency *F \*G bk1 << fy << bk2 *F \*i Df(2L)TE128X15 *F \*E FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*p No effect on wm4h position-effect variegation. *F \*i Df(2L)TE128x15 *F \# *F \*a Df(2L)TE29Aa-16 *F \*z FBab0001572 *F \*H Not updated after 20 May 94 *F \*x Ashburner, unpubl. *F \*x FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*x FBrf0065509 == rd1177.mg == Neuman-Silberberg and Schupbach, 1993, Cell 75(1): 165--174 *F \*B 28F2-29A1;29D2-29E1 *F \*C Deficiency *F \*G bk1 << fy << bk2 *F \*i Df(2L)TE128X16 *F \*E FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*p Enhances of wm4h position-effect variegation. *F \*i Df(2L)TE128x16 *F \*E FBrf0065509 == rd1177.mg == Neuman-Silberberg and Schupbach, 1993, Cell 75(1): 165--174 *F \*q Deletes or disrupts grk *F \*i Df(2L)TE128x16 *F \# *F \*a Df(2L)TE29Aa-38 *F \*z FBab0001575 *F \*H Not updated after 20 May 94 *F \*x Ashburner, unpubl. *F \*x FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*B 29A2-29B1;29F1-29F2 *F \*C Deficiency *F \*G bk1 << fy << bk2 *F \*i Df(2L)TE128X38 *F \*E FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*p No effect on wm4h position-effect variegation. *F \*i Df(2L)TE128x38 *F \# *F \*a Df(2L)TE29Aa-41 *F \*z FBab0001576 *F \*H Not updated after 20 May 94 *F \*x Ashburner, unpubl. *F \*x FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*x FBrf0065509 == rd1177.mg == Neuman-Silberberg and Schupbach, 1993, Cell 75(1): 165--174 *F \*B 29A1-29A2;29E1-29E2 *F \*C Deficiency *F \*G bk1 << fy << bk2 *F \*i Df(2L)TE128X41 *F \*E FBrf0050576 == ew265 == Wustmann et al., 1989, Molec. gen. Genet. 217: 520--527 *F \*p Enhances of wm4h position-effect variegation. *F \*i Df(2L)TE128x41 *F \*E FBrf0065509 == rd1177.mg == Neuman-Silberberg and Schupbach, 1993, Cell 75(1): 165--174 *F \*q Deletes or disrupts grk *F \*i Df(2L)TE148x41 *F \# *F \*a fy *F \*H Last updated 14 Nov 94 *F \*e fuzzy *F \*z FBgn0001084 *F \*b 2-24.1 *F \*c 29C1--29C2 *F \*w Ives, Jan. 1939 *F \*x FBrf0068246 == Collier and Gubb, 1994, A. Conf. Dros. Res. 35 : 281 *F \*x FBrf0063488 == Grell, 1969, D. I. S. 44: 46--47 *F \*x FBrf0045354 == Held et al., 1986, Roux Arch. dev. Biol. 195: 145--157 *F \*x Henchcliffe, pers.comm *F \*x FBrf0065666 == Ives, 1940, D. I. S. 13: 49--50 *F \*x FBrf0066905 == Lindsley and Zimm, 1992 *F \*x FBrf0078233 == ew1659 == Collier and Gubb, 1995, A. Conf. Dros. Res. 36: 32B *F \*x FBrf0078828 == rd1628 == Krasnow and Adler, 1995, A. Conf. Dros. Res. 36: 260A *F \*A fy1 *F \*z FBal0004915 *F \*o spontaneous *F \*k Hairs on abdomen and thorax irregular and directed *F \*k toward midline. Hairs on wing margins erect. Hairs on *F \*k legs also show abnormal polarities (Held, Duarte, and *F \*k Derakhshanian, 1986). Resembles fz. Fertility and viability *F \*k below normal. *F \*k RK2. *F \*A fy2 *F \*z FBal0004916 *F \*x FBrf0063488 == Grell, 1969, D. I. S. 44: 46--47 *F \# # *U FBrf0080761 *a Hursh *b D. *t 1995.7.5 *T personal communication to FlyBase *u *F From Hurshd@dc37a.nci.nih.gov Wed Jul 5 18:53:13 1995 *F From: 'Hursh, Deb' *F To: ma11@gen.cam.ac.uk *F Subject: help FlyBase please *F Date: Wed, 05 Jul 95 13:46:00 edt *F Encoding: 12 TEXT *F X-Mailer: Microsoft Mail V3.0 *F Content-Length: 781 *F *F Dear Michael, *F Yes, these data must have came off a poster in 1987. I was *F unaware they were even in there until years later. I probably cannot *F completely satisfy your request, unfortunately. The *F data are not correct. The sequence was subsequently identified as a putative *F homolog of Saccharomyces ribosomal protein L-25. The *F sequence is unpublished, and there is no Accession number, although I hope to *F remedy this soon. There is no bibliographic citation; only my sea urchin *F work showed up in my dissertation. Personal communication seems the only *F recourse. The cytology is correct, by in situ hybridization with cDNA clones. *F I toil on this in my spare time- you can see it has not moved too fast. I *F hope this (somewhat) clears up the record. Deb Hursh # *U FBrf0080762 *a Ashburner *b M. *t 1995.7.10 *T personal communication to FlyBase *u *F Tp(2;3)I709 was sent to us by Ising in the early 1970's. It was induced on In(3L)P *F and I did the cytology on 13-4-1971 & this was checked for Gary Struhl on 16-4-1981 *F \*B 31B2-5;34A1-7;34D6-8;63C;72E1-2;76B5-C1 *F \*N 21 - 31B2 | 34D8 - 60; 61 - 63C | 72E1 - 63C | 72E2 - 76B5 | 34A7 - 34D6 | 31B5 - 34A1 | 76C1 - 100 *F *F Tp(3;2)I702 was also from Ising and also on In(3L)P and I did the cytology on *F 13-4-1971. *F *F \*B 29F;64F;63C;72E1-2;77F *F \*N 21 - 29F | 77F - 72E2 | 63C - 64F | 29F - 60; 61 - 63C | 72E1 - 64F | 77F - 100 # *U FBrf0080763 *a Seeger *b M. *t 1995.7.6 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Mark Seeger, University of Oklahoma Health Sciences Center *F To: Bloomington Drosophila Stock Center *F Subject: Df(3L)fz-M21 *F Date: 6 July 1995 *F *F Information communicated: *F *F Df(3L)fz-M21 deletes commissureless, which is at 71E3-5. # *U FBrf0080775 *a Guild *b G. *t 1995.7.12 *T personal communication to FlyBase *u *F From gguild@sas.upenn.edu Wed Jul 12 15:01:02 1995 *F Posted-Date: Wed, 12 Jul 1995 09:51:02 -0400 *F X-Sender: gguild@postoffice.sas.upenn.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 12 Jul 1995 09:59:34 -0500 *F To: ma11 *F From: gguild@sas.upenn.edu (Greg Guild) *F Subject: 71E genes *F Content-Length: 1915 *F *F Hi Michael, *F *F I'm terribly sorry to take so long in responding to your question. Yes, we *F have cloned out a few more genes in the 71E region and sequenced the whole *F area. We will send the revised manuscript back to J. Mol. Biol. this month *F and I'll send a copy to you too. In any event, here is the conversion *F chart: *F *F New Name (1) Old Name (2) Genbank FlyBase *F L71-1 transcript-I U24095 Eig71Ea *F L71-2 transcript-II U24149 Eig71Eb *F L71-3 transcript-III U24242 Eig71Ec *F L71-4 transcript-IV U24243 Eig71Ed *F L71-5 transcript-V U24244 Eig71Ef *F L71-6 transcript-VI U24245 Eig71Eg *F I71-7 transcript-VII U24246 Eig71Ee *F L71-8 U24572 *F L71-9 U24573 *F L71-10 U24574 *F L71-11 U24575 *F *F (1) From a manuscript submitted to J. Mol. Biol. (L) Late gene; (I) *F Intermolt gene. We found 4 more late genes to the right of the old *F cluster. We are now revising the MS which contains all the sequence data *F and its analysis. *F *F Wright, L.G., Chen, T., Thummel, C.S. and Guild, G.M. Molecular *F characterization of the 71E late puff in Drosophila melanogaster reveals a *F family of novel genes. (submitted). *F *F (2) Restifo, L.L. and Guild, G.M. (1986). An ecdysterone-responsive puff *F site in Drosophila contains a cluster of seven differentially regulated *F genes. J. Mol. Biol. 188, 517-528. *F *F Hope this clears things up a bit. again, I apologize for the lateness of *F my response. *F *F Greg *F ______________________________________________________________________________ *F Greg Guild Phone: (215) 898-3433 *F Department of Biology FAX: (215) 898-8780 *F University of Pennsylvania E-mail: gguild@sas.upenn.edu *F Philadelphia, PA 19104-6018 USA # *U FBrf0081397 *a Baumgartner *b S. *t 1995.6.22 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed Jun 21 14:08:11 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 21 Jun 95 14:08:07 BST *F To: baumgart@fmi.ch *F Subject: Which Tenascin gene? *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 462 *F Dear S. Baumgartner, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F I am curating the abstracts from the fifth European meeting on the *F neurogenetics of Drosophila. Your abstract on page 18 discusses PCRs *F derived from EGF-like region of tenascin. *F Which tenascin gene are you using? *F This information is vital for completion of the abstract curation so *F your help would be invaluable. *F Many thanks, *F Eleanor Whitfield. *F From baumgart@fmi.ch Thu Jun 22 08:18:47 1995 *F To: Eleanor Whitfield (Genetics) *F Cc: baumgart@fmi.ch, baumgart@fmi.ch *F Subject: Which Tenascin gene? *F Date: Thu, 22 Jun 95 09:15:19 +0200 *F From: baumgart@fmi.ch *F X-Mts: smtp *F Content-Length: 450 *F \-------- *F Dear Eleanor, *F The primers were used from chicken tenascin, but since neurexin (nrx) has *F EGF-like repeats as well, therefore, it is considered an accidential *F amplification. The nrx gene resides at 68F3-6, gives a transcript of 5.0 kb, and *F encodes a protein of 1283 aa with an apparent MW of about 155 kD with a domain *F structure almost identical to vertebrate neurexin. *F Hope this helps to complete the abstract curation. *F Regards Stefan # *U FBrf0081398 *a Baumgartner *b S. *t 1995.7.4 *T personal communication to FlyBase *u *F Archived # *U FBrf0081489 *a Brand *b A. *t 1995.8.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Jul 11 11:44:30 1995 *F From: Rachel Drysdale (Genetics) *F Date: Tue, 11 Jul 95 11:39:49 BST *F To: ahb@mbuk.bio.cam.ac.uk *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk, eleanor@gen.cam.ac.uk *F Content-Length: 986 *F *F Hi Andrea! *F *F I bet you can help us. *F *F We have a question about your Brand and Perrimon 1993 Development *F 118:401-415 paper. *F *F In the Materials and Methods, in paragraph 2 of the section titles *F 'Enhancer Detection Screen' you mention an insertion on the X that you *F term 'GAL4-lethal'. *F *F We would like to record this element in FlyBase. Do you have any more *F information about this lethal insert? Such as its cytological location? *F Failing that, do you have an insertion designation that you use for *F it? *F *F Many thanks for your help. *F *F best wishes, *F Hope you are keeping cooler than we are. *F Rachel *F *F -------------------------------------------------------------- *F Rachel Drysdale. *F *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F -------------------------------------------------------------- *F From rd120@gen.cam.ac.uk Fri Jul 21 10:48:33 1995 *F From: Rachel Drysdale (Genetics) *F Date: Fri, 21 Jul 95 10:43:32 BST *F To: ahb@mbuk.bio.cam.ac.uk *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk, eleanor@gen.cam.ac.uk *F Content-Length: 2593 *F *F Hi Andrea, *F *F here's the deal. The paper is: *F *F \# *F \*U 76506 *F \*a Fuse *F \*b N. *F \*c S. *F \*d Hirose *F \*e S. *F \*f Hayashi *F \*t 1994 *F \*u Diploidy of Drosophila imaginal cells is maintained by a transcriptional repressor encoded by escargot. *F \*v *F \*w Genes Dev. *F \*x *F \*y 8 *F \*z 2270--2281 *F \*Y 19 *F \*M 95047371 *F \# *F *F In it they describe a salivary gland expression pattern (p2274) for a *F GAL4 insert. They give no data on which one. When we queried them (Hayashi) *F they said 'We used the line designated Gal4-lethal/FM7 which Brand and *F Perrimon used as a starting point for their enhancer trap screen' which *F seems fairly unambiguous. *F *F What do you reckon? *F *F Do you know that the insertion was responsible for the lethal *F phenotype? Were there revertants in your screen, or is that something *F that you didn't score? If the insert does cause the lethality we could *F call the insertion P{GAL4}l(1)BP[1] for 'Brand and Perrimon'. Since *F symbols for lethal mutations should reflect either a mutant phenotype *F or a cytological location, this l(1)BP gene would be renamed when either *F a cytological location or a phenotypic name when ever anyone published *F such a thing. If you don't know that the element caused the lethality, *F then we can should refer to the insert as P{GAL4}BP, or with a *F line/stock designation if you have managed to find one. *F *F Let me know what you think *F *F cheers *F *F Rachel *F *F From ahb@mole.bio.cam.ac.uk Tue Aug 8 12:48:34 1995 *F Date: Tue, 8 Aug 95 12:44:52 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: rd120 *F From: ahb@mole.bio.cam.ac.uk (Andrea Brand) *F Subject: Re: helping FlyBase *F Content-Length: 87 *F *F Hi Rachel, *F Sorry for the delay - P{GAL4} l(1)BP[1] is fine. *F Thanks, *F Andrea. # *U FBrf0081594 *a Chartoff *b E.H. *c J.J. *d Sekelsky *e W.M. *f Gelbart *t 1995.8.10 *T personal communication to FlyBase *u *F Identification of a Drosophila Gene With Sequence Similarity to the Predicted *F Human Gene pHPS[[1]]-2 *F *F Elena H. Chartoff, Jeff J. Sekelsky, William M. Gelbart *F *F As part of the molecular analysis of the Drosophila melanogaster gene Mothers *F against dpp (Mad), we cloned genomic DNA flanking the insertion site of *F P{walter}(23D) (1), a P element inserted into Mad (2). Two transcription units *F near P{walter}(23D) were identified by screening a cDNA library made from *F early embryonic RNA (3) and probing blots of early embryonic RNA. Germline *F transformation with rescue constructs indicated that one of these *F transcription units corresponds to Mad (2). From the other transcription unit, *F we sequenced a representative 2.4 kb cDNA, designated C24, that lies at *F position +17 on the molecular map of the Mad region, approximately 6 kb *F proximal to the P{walter}(23D) insertion point (2). We refer to the gene *F represented by C24 as anon-23D. *F *F The 2255 bp sequence of C24 contains one long open reading frame (ORF) from *F base 272 to 1465 (accession \# U29170). The codon frequency in this ORF is *F similar to that of other Drosophila melanogaster genes (4), and the sequence *F surrounding the putative translation start is a good match to the weak *F consensus for translation initiation in Drosophila (5). Conceptual translation *F of this ORF predicts a polypeptide with 398 amino acid residues. A search of *F protein databases using the Basic Local Alignment Search Tool (6) revealed one *F sequence, pHPS[[1]]-2 (accession \# P08910; 7), with strong similarity to C24. *F The Poisson probability of pHPS[[1-2]] and C24 sequences matching by chance *F alone was computed to be below 10[-125]. The pHPS[[1]]-2 sequence was *F predicted from the sequence of a human cDNA clone fortuitously isolated in a *F screen for G-protein-linked receptors (7). Although the function of *F pHPS[[1]]-2 is unknown, it shows weak similarity to G protein-linked *F receptors. C24 is 43% identical and 64% similar to pHPS[[1]]-2 over its entire *F length (Figure 1). Both sequences are extremely hydrophobic and have a signal *F secretion sequence and at least one region predicted to be membrane-spanning. *F *F This work was supported by a research grant from the NIH to W.M.G. *F *F 1 .............MSTAFLTLIAVIVCILFRILNVHSQPLKPSVWCLDAH *F 3 :3 :: :: 3 33::3 3 :: 3 *F 1 MNAMLETPELPAVFDGVKLAAVAAVLYVIVRCLNLKSPTAPPDLYFQDSG *F *F 38 FLDCLYKIAPVLREPYIPPRLWGFSGHVQTVLHSIVGRVRCPWPLGERVY *F 3 3 3:3 3333 :33 333:33 3 3333 3 3 3 3 : *F 51 LSRFLLKSCPLLTKEYIPPLIWGKSGHIQTALYGKMGRVRSPHPYGHRKF *F *F 88 MSLKDGSTLTYDLYQPLNEQ..EDDITVAICPGIANSSESVYIRTFVHLA *F ::: 33 3 3:33: 33 3 3333: 3333333 33 333333 3 *F 101 ITMSDGATSTFDLFEPLAEHCVGDDITMVICPGIANHSEKQYIRTFVDYA *F *F 136 QCNGYRCAVLNHIGALRSVQVTSTRIFTYGHTEDFAAMVEHLHQKYRQSR *F 3 3333333333:333 : :33 3:3333 3 :3:333 :: 3 : *F 151 QKNGYRCAVLNHLGALPNIELTSPRMFTYGCTWEFGAMVNYIKKTYPLTQ *F *F 186 IVAVGFSLGGNLVTKYMGEDQKTKPNKVIGGISICQGINAVEGTKWLLNW *F :3 33333333:3 33:33 3 33: :3:333 3: : : 3 *F 201 LVVVGFSLGGNIVCKYLGETQ.ANQEKVLCCVSVCQGYSALRAQETFMQW *F *F 236 QNFRRFYLYIMTENVKSIILRHRHILLSDEVKARHNLNEREI...IAAAT *F 3333 ::3 :3:3 333 33 3 3 33 3 : :: 3 : *F 250 DQCRRFYNFLMADNMKKIILSHRQALFGDHVKKPQSLEDTDLSRLYTATS *F *F 283 LPELDEAYTRRVYNFPSTQELYKWSSSLFYFDTIKKPMIFINAKDDPLIP *F 3 :3: 3: : 3 3 3 3 : 3 3 3:: :33 3333: *F 300 LMQIDDNVMRKFHGYNSLKEYYEEESCMRYLHRIYVPLMLVNAADDPLVH *F *F 333 EDLLHPIKEYATTRQNTAYVEVAHGGHLGFYEGGFLYPNPVTWLDRTLVA *F 3 33 3 3 3 :3 3333333:33 3:3 3:33:3: :3 *F 350 ESLLTIPKSLSEKRENVMFVLPLHGGHLGFFEGSVLFPEPLTWMDKLVVE *F *F 383 MVGSLVMMHEVGKVAP 399 *F : 3 3: *F 400 YANAIC.QWERNKLQCSDTEQVEADLE 425 *F *F Figure 1. Sequence similarity between C24 and pHSPS[[1]]-2. *F *F The predicted C24 sequence is aligned above that of pHSPS[[1]]-2. Solid lines *F indicate identical residues; dotted lines indicate similar residues. Gaps *F introduced into the sequences to maximize sequence similarity are indicated by *F periods. The initial alignment was produced with the GCG program GAP (8). The *F output was edited manually to reflect biochemical similarities rather than *F codon similarities. *F *F References *F *F 1. Gloor, G.B., N.A. Nassif, D.M. Johnson-Schlitz, C.R. Preston, and W.R. Engels *F (1991). Science 253: 1110-1117. *F *F 2. Sekelsky, J.J., S.J. Newfeld, L.A. Raftery, E.H. Chartoff, and W.M. Gelbart *F (1995). Genetics 139: 1347-1358. *F *F 3. Brown, N.H. and F.C. Kafatos (1988). J. Mol. Biol. 203: 425-437. *F *F 4. Ashburner, M. (1989). Drosophila - A Laboratory Handbook. Cold Spring Harbor *F Laboratory Press, Cold Spring Harbor, NY. *F *F 5. Cavener, D.R. and S.C. Ray (1991). Nucleic Acids Research 19: 3185-3192. *F *F 6. Altschul, S.F., W. Gish, W. Miller, E.W. Meyers, and D.J. Lipman (1990). J. *F Mol. Biol. 215: 403-410. *F *F 7. Rapiejko, P.J., S.T. George, and C.C. Malbon (1988). Nucleic Acids Res. 16: *F 8721. *F *F 8. Devereux, J., P. Haeberli, and O. Smithies (1984). Nucleic Acids Res. 12: *F 387-395. # *U FBrf0081986 *a Fehon *b R. *t 1995.7.10 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon Jul 10 08:36:55 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 10 Jul 95 08:36:48 BST *F To: rfehon@acpub.duke.edu *F Subject: 36th ADRC, abstract 17A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 562 *F *F Dear Dr. Fehon, *F *F I am curator of FlyBase working at the Cambridge, England, site with *F Prof M. Ashburner. *F *F I have curated your abstract 17A from the 36th ARDC and it has come to *F my attention that in the genes file we have: *F *F \*a Emr1 *F \*e Ezrin-moesin-radixin-1 *F \*d moesin-like *F \*d ezrin-like *F \*d radixin-like *F \*g U23798 *F *F \*a Emr2 *F \*e Ezrin-moesin-radixin-2 *F \*d moesin-like *F \*d ezrin-like *F \*d radixin-like *F \*g U23799 *F *F Could you tell me if the gene Dmoesin in your abstract corresponds to *F one of these or if it is a different gene altogether. *F *F Many thanks, *F Eleanor Whitfield *F *F From rfehon@acpub.duke.edu Mon Jul 10 15:19:11 1995 *F Date: Mon, 10 Jul 1995 10:15:50 -0400 *F X-Sender: rfehon@popserv.acpub.duke.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: rfehon@acpub.duke.edu *F Subject: Re: 36th ADRC, abstract 17A *F Content-Length: 1107 *F *F To Eleanor Whitfield: *F *F Thanks for your note. The sequence U23798 is a partial sequence of the *F Dmoesin gene that is described in abstract 17A from the 36th ARDC. We have *F published the complete sequence of this gene in genbank and its accession *F number is L38909. You didn't ask, but U23799 is a partial sequence of the *F Dmerlin gene that we describe in the same abstract. We have not yet *F submitted this sequence to genbank, but hope to do so shortly. *F *F I hope this clears up the confusion. *F Sincerely, Rick Fehon. *F *F From eleanor@gen.cam.ac.uk Mon Jul 10 15:36:45 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 10 Jul 95 15:36:43 BST *F To: rfehon@acpub.duke.edu *F Subject: Re: 36th ADRC, abstract 17A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 263 *F *F Dear Rick, *F *F Many thanks for your reply, it provided more information than I asked *F for and because of that probably prevented a further query! *F As a result of your information I shall now curate Dmoesin under Emr1 *F and Dmerlin under Emr2 *F *F Regards, *F Eleanor Whitfield *F *F From rfehon@acpub.duke.edu Mon Jul 10 19:31:00 1995 *F Date: Mon, 10 Jul 1995 14:27:41 -0400 *F X-Sender: rfehon@popserv.acpub.duke.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: rfehon@acpub.duke.edu *F Subject: Re: 36th ADRC, abstract 17A *F Content-Length: 601 *F *F To Eleanor Whitfield *F *F One more piece of information that you didn't ask for. Another partial *F Dmoesin sequence has been published by my colleague Dan Kiehart's group *F under accession \# U08218. To my knowledge, there are no other copies of *F that sequence in the database (ours is the only complete sequence though). *F Rick Fehon. *F *F From eleanor@gen.cam.ac.uk Tue Jul 11 09:20:51 1995 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 11 Jul 95 09:20:48 BST *F To: rfehon@acpub.duke.edu *F Subject: Re: 36th ADRC, abstract 17A *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1627 *F *F Hi Rick, *F *F >One more piece of information that you didn't ask for. Another partial *F Dmoesin sequence has been published by my colleague Dan Kiehart's group *F under accession \# U08218. *F *F Another layer of complexity! *F From your first message Dmoesin is Emr1 and therefore should become a *F synonym. *F \*a Emr1 *F \*e Ezrin-moesin-radixin-1 *F \*d moesin-like *F \*d ezrin-like *F \*d radixin-like *F \*g U23798 *F *F In this second message you say that accession number U08218 is a *F partial Dmoesin sequence, we have this accession number under Moe. *F \*a Moe *F \*e Moesin-like *F \*b 1-[27] *F \*c 8B *F \*d moesin-like *F \*d ezrin-like *F \*d radixin-like *F \*g U08218 *F \*g L38909 *F \*x FBrf0073018 == Edwards et al., 1994, Proc. natn. Acad. Sci. USA. 91(10): 4589--4593 *F \*E FBrf0073018 == Edwards et al., 1994, Proc. natn. Acad. Sci. USA. 91(10): 4589--4593 *F \*i D17 *F \*p A yeast phenotypic screen efficiently identifies conserved genes from *F \*p more complex organisms and sheds light on their potential in vivo functions. *F \*p Induced expression of @Moe@ proteins causes aberrant cell shapes reflecting *F \*p defects in cytokinesis and/or cell shape maintenance. *F *F The accession number U08218 is published in a 1994 paper, Emr1 will be *F in a 1995 paper (the sequence report states the paper has been *F submitted by P. Winge but gives no journal and we do not have any *F details yet). So because of the rule of precedence this is means: *F gene symbol: Moe *F synonyms: Emr1 *F Dmoesin *F *F Two genes have now successfully been merged - thank you very much. *F *F Eleanor Whitfield. # *U FBrf0082039 *a Gonzalez-Reyes *b A. *t 1995.8.1 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Acaimo Gonzalez-Reyes, Wellcome/CRC Institute *F To: Bloomington Drosophila Stock Center *F Subject: P{hsneo}l(3)neo60[1] *F Date: 1 August 1995 *F *F Information communicated: *F *F "According to Steve Wasserman, they have refined the mapping of this P-element to 97F (compared to 97E-F in FlyBase)." # *U FBrf0082054 *a Guastella *b J. *t 1995.8.25 *T personal communication to FlyBase *u *F Archived # *U FBrf0082163 *a Hultmark *b D. *t 1995.3.15 *T personal communication to FlyBase *u *F Archived # *U FBrf0082216 *a Kennison *b J. *t 1995.6.21 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Jim Kennison, NIH *F Subject: complementation data for assorted P{} insertions in the Bloomington collection *F Dated: 21 Jun 95 *F *F Information communicated: *F *F P{ry11}l(3)ry16[1] complements Df(3R)Scr and Df(3R)Antp17 *F P{ry11}l(3)ry160[1] complements Df(3R)Scr and Df(3R)Antp17 *F P{PZ}lab[01241] complements Df(3R)Antp17; fails to complement Df(3R)Scr and lab[1] *F P{PZ}srp[01549] fails to complement Df(3R)sbd105 and srp[3]; complements Df(3R)Po4, Df(3R)sbd45, and mor[1]. *F P{PZ}l(3)72Dd[03802] fails to complement Df(3L)th102, Df(3L)th117, Df(3L)st-e4, and l(3)72Dd[3]; complements Df(3L)brm11. *F P{PZ}twr[05614] fails to complement Df(3R)Scr and twr[4]; complements Df(3R)Antp17. *F P{PZ}l(3)08724[08724] viable but male sterile with Df(3R)sbd105; complements Df(3R)Po4, Df(3R)sbd45, mor[1], and srp[3]. *F P{PZ}ms(3)72D[03957] complements Df(3L)th102. *F P{PZ}ms(3)04895[04895] complements Df(3R)sbd105. *F P{lacW}l(3)j5C8[j5C8] fails to complement Df(3L)th102, Df(3L)th117, and th[4] (a lethal thread allele). *F P{lacW}l(3)s1939[s1939] complements Df(3L)th102. *F P{lacW}l(3)j5A4[j5A4] fails to complement Df(3L)th102, Df(3L)st4, Df(3L)st-e4, and l(3)72Dn[1]; complements Df(3L)brm11, Df(3L)st-b11, and l(3)72Do[1]. *F P{lacW}l(3)j3D4[j3D4] fails to complement Df(3L)kto2 and P958. *F P{PZ}l(2)06751[06751] complements kis[1]. # *U FBrf0082231 *a Klaembt *b C. *t 1995.8.1 *T personal communication to FlyBase *u *F From cklaemb@cipvax Tue Aug 1 14:34:13 1995 *F Date: Tue, 1 Aug 95 14:34:10 BST *F From: 'Christian Klaembt' *F Reply-To: 'Christian Klaembt' *F To: rd120@gen.cam.ac.uk *F Subject: GAL4 lines, your fax from the 12th July *F Content-Length: 528 *F Dear Rachel, *F The sca-GAL4 line is a GAL4 enhancer trap insertion in the scabrous gene. It was *F generated by Dr. U. Hinz, Koeln. It causes a very weak hypomorphic sca *F phenotype. *F The rho-GAL4 line is a promotor-GAL4 line generated in M.Levin«s lab. *F There are no further references to these lines to my knowledge. *F I hope this information is sufficient, *F best wishes Christian *F Dr. Christian Klaembt *F Institut fuer Entwicklungsbiologie *F Universitaet zu Koeln *F D 50923 Koeln *F Tel: (x)49-221-470-3130 FAX: (x)49-221-470-5164 # *U FBrf0082285 *a Lindsley *b D.L. *t 1995.9.6 *T personal communication to FlyBase *u *F From dlindsley@ucsd.edu Wed Sep 6 19:04:56 1995 *F Date: Wed, 6 Sep 1995 10:01:01 -0800 *F To: ma11 *F From: dlindsley@ucsd.edu (Dan Lindsley) *F X-Sender: redbook@jeeves.ucsd.edu *F Subject: Re: help please *F Content-Length: 570 *F *F In looking back at the computerized stock list that we prepared in 1982, I *F see that La Jolla had a stock labeled C(1)M3, y[2] bb/w[ch]; SM2/Su(w[ch]). *F Therefore, I suspect that the '2' in w[ch2] is an adventitious error in *F labeling. *F *F >Dan *F > *F >There is the following stock in Bloomington: *F > *F > *F >3706,'w[ch2]; SM1/Su(w[ch2])[1]','1;2','',9/01/1991,'Dan Lindsley','','' *F > *F >FlyBase has Su(w[ch]) but not Su(w[wh2]). We will assume that they are *F >different genes unless you tell us otherwise ! # *U FBrf0082331 *a Matthews *b K. *t 1995.8.18 *T personal communication to FlyBase *u *F Personal communication from: Kathy Matthews, Indiana University *F To: FlyBase *F Subject: betaTub85D[n] *F Dated: 18 Aug 95 *F *F Information communicated: *F betaTub85D[n] was recovered over Df(3R)by62, a deficiency that does not remove the betaTub85D locus. # *U FBrf0082380 *a Mottus *b R. *t 1995.7.20 *T personal communication to FlyBase *u *F Fax and email from Randy Mottus, University of British Columbia, July 20 1995. *F Copy available from FlyBase. *F From mottus@bcu.ubc.ca Fri Jul 21 17:26:59 1995 *F X-Host-Id: light.bcu.ubc.ca *F Date: Fri, 21 Jul 1995 08:32:15 -0700 (PDT) *F From: Randy Mottus *F Subject: Re: FlyBase *F To: ma11 *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 156 *F *F Dear Michael *F *F I believe that da20 aka 77-11 was recovered over Df(2L)J77. If this is *F not the case I will drop you a line. *F *F Glad to be of help *F *F Randy # *U FBrf0082395 *a Munoz *b E. *t 1992.12.16 *T personal communication to FlyBase *u *F Archived # *U FBrf0082406 *a Niznik *b H.B. *t 1995.8.24 *T personal communication to FlyBase *u *F Fax from H.B. Niznik 24 August 1995 to FlyBase stating that the Demchyshyn et *F al. sequence (U04808) and that of Corey et al. (U02296) are from the same gene, *F despite difference in translation at N terminus due to a missing C in U02296. *F *F Copy available from FlyBase. # *U FBrf0082535 *a Roote *b J. *t 1995.9.1 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Fri Sep 1 17:24:16 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 1 Sep 1995 17:20:07 +0100 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: help flybase please *F Content-Length: 1028 *F *F These are the extant Dp(2;2)Cam's. *F *F *F 2-216 y[+]Y; Dp(2;2)DTD8[L] S325[R]/In(2LR)O, Cy dp[lvI] pr cn bw *F *F Dp(2;2)Cam1 Dp 21F;23C-D *F *F *F 2-223 Dp(2;2)DTD24[L] DTD8[R], sp/In(2LR)O, Cy dp[lvI] pr cn bw *F Dp(2;2)Cam2 Dp *F 23C-D;26C12 *F *F *F 2-351 Dp(2;2)DTD111[L] DTD24[R], bw sp/In(2LR)O, Cy dp[lvI] pr cn[2] *F Dp(2;2)Cam3 Dp 26C12;29F *F *F *F 2-366 Dp(2;2)DTD107[L] DTD111[R], In(2L)DTD107, TE23CD vg/In(2LR)O, Cy *F dp[lvI] pr cn[2] *F Dp(2;2)Cam4 Dp 29F;32F *F *F *F 2-319 Dp(2;2)lt[G16L] Pu[LR], b bw/In(2LR)O, Cy dp[lvI] pr cn[2] *F Dp(2;2)Cam14 Dp(2;2)57F;60E *F *F *F 2-320 (y w); Dp(2;2)TE35B-GR226[L] TE35B-SZ4[R], b pr l(2)pwn/In(2LR)O, *F Cy dp[lvI] b rk pr cn[2] *F Dp(2;2)Cam11 Dp(2;2)43A;47B *F *F *F 2-53 Dp(2;2)C3[L] CH9-25[R], b/In(2L)Cy In(2R)Cy, al[2] Cy pr Bl cn[2] *F vg c sp[2] *F Dp(2;2)Cam8 Dp(2;2)36;40 *F *F *F 2-58 Dp(2;2)D29[L] CH9-25[R], b/In(2L)Cy In(2R)Cy, al[2] Cy pr Bl cn[2] *F vg c sp[2] *F Dp(2;2)Cam8a Dp(2;2)36;40 *F *F *F 2-302 In(2LR)DTD128[L] TE35B-GR226[R], net ho[2] TE23CD/In(2LR)O, Cy *F dp[lvI] pr cn[2] *F Dp(2;2)Cam13 Dp 47B;48C *F *F *F *F 2-203 C(2L)C3, b pr; C(2R)C1, sple *F origin: C3 and C1 from b pr sple irrad, X D29//VK1 *F David Gubb *F *F *F 2-403 LS(2)D5//DS(2)CH9-25, cn *F origin: CH9 from irrad S325//S325 X C3//C1 *F C. Henchcliffe, 89 # *U FBrf0082536 *a Roote *b J. *t 1995.9.4 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Sep 4 09:39:09 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 4 Sep 1995 09:34:57 +0100 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: abs *F Content-Length: 1286 *F *F I have an early draft of the S-U, Gelbart paper in which Table 6 list the *F DTD's with names of this type, plus the translation to the published name. *F *F 22.18 does not exist _but_ DTD25.18 = DTD121 = In(2LR)35B;41;42A;56F (i.e. *F the cytology that Flybase has for DTD22.18). *F *F DTD22.59 = DTD113 = T(2;3)24A1.2;68E1.2 *F DTD25.59 = DTD128 = In(2LR)35C;46A (Gelbart) or 35B1-3;48C6-8 (Ashburner). *F *F J # *U FBrf0082537 *a Roote *b J. *t 1995.9.6 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Sep 6 11:47:10 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 6 Sep 1995 11:43:09 +0100 *F To: Michael Ashburner *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: C(2L) etc. *F Content-Length: 956 *F *F Origin of Gubb's compound 2's: *F *F C(2L)C3, b pr from b pr sple gamma-irrad females X D29//VK1 males *F C(2R)C1, sple ditto *F C(2R)C2, sple ditto *F *F C(2L)C5, Cy from irrad C(1)RM; In(2L+2R)Cy/Df(2R)MS-10 X C(2L)C3//C(2R)C1 # *U FBrf0082623 *a Smolik *b S. *t 1995.7.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Jul 12 17:34:43 1995 *F From: Rachel Drysdale (Genetics) *F Date: Wed, 12 Jul 95 17:29:57 BST *F To: rd120@gen.cam.ac.uk *F Subject: Creb *F Cc: smoliks@ohsu.edu *F Content-Length: 361 *F Rachel, *F Jerry Yin's Creb is NOT the same one as \*a Creb currently in FlyBase. *F It is dCREB-B (aka (d)CREB-2) with different isoforms a, b, ...., *F (alternative splice variants) and this and a close relative maps to 17A *F and 17B. But which is which is not known. It is not even clear that *F the different isoforms all come from the same gene. *F Sarah Smolik # *U FBrf0082628 *a Spradling *b A. *t 1995.8.11 *T personal communication to FlyBase *u *F From: Rachel Drysdale (Genetics) *F Date: Wed, 9 Aug 95 20:40:39 BST *F Message-Id: <9508091940.AA19180@hmg1.gen.cam.ac.uk> *F To: spradling@mail1.ciwemb.edu *F Subject: helping flybase! *F Cc: rd120@gen.cam.ac.uk *F *F Hi Allan, *F I'm doing some work on your 1987 paper: *F Spradling et al., 1987, EMBO J. 6: 1045--1053 *F In Figure 1 you show the X chromosome Cp36 and Cp38 genes, and some *F other transcripts, A (1200), B (1650), C (960), E (1350) and K (3200). *F The numbers in parentheses refer to the bp length identifiers for these *F given in: *F Wakimoto et al., 1985, Gametogenesis and the Early Embryo. 44th *F Symposium of the Society of Developmental Biology, Toronto, Canada, *F June 13-15, 1985 (Ed. Gall,J.G.): 43--54 *F Can you tell me if any if these egg-chamber-specific transcripts *F correspond to any named gene? *F I found the following candidates in the Encyclopaedia: *F *F \*a fs(1)&ggr;a6 *F \*c 7E10--8A3 *F \*x FBrf0063948 == ew757 == Wakimoto and Spradling, 1982, Yb. Carnegie Inst., *F Wash. 81: 190 *F \# *F \*a fs(1)&ggr;a7 *F \*c 7E10--8A3 *F \*x FBrf0063948 == ew757 == Wakimoto and Spradling, 1982, Yb. Carnegie Inst., *F Wash. 81: 190 *F \*# *F \*a fs(1)&ggr;a8 *F \*c 7E10--8A3 *F \*x FBrf0063948 == ew757 == Wakimoto and Spradling, 1982, Yb. Carnegie Inst., *F Wash. 81: 190 *F \# *F \*a fs(1)2448 *F \*c 7E10--8A3 *F \*x FBrf0063948 == ew757 == Wakimoto and Spradling, 1982, Yb. Carnegie Inst., *F Wash. 81: 190 *F \# *F \*a fs(1)B4 *F \*c 7E10--8A3 *F \*x FBrf0063948 == ew757 == Wakimoto and Spradling, 1982, Yb. Carnegie Inst., *F Wash. 81: 190 *F \# *F \*a pt *F \*i l(1)7Fb *F \*i fs(1)M47 *F \*c Located in the 7F region since *F \*c included in Df(1)7F1-2;8C6; maps to the left of oc, *F \*c which is located at 8A1-2. *F \*c included in Df(1)KA14 *F \*x FBrf0063948 == ew757 == Wakimoto and Spradling, 1982, Yb. Carnegie Inst., *F Wash. 81: 190 *F \# *F *F Do any of these correspond to any of the transcripts in a one-to-one way? *F best wishes *F *F Rachel *F *F Dear Rachel, *F The transcripts define 3 new presumed low abundance chorion protein genes: *F 1. A ; 2. B and C ; 3. D and E *F These genes were never named or sequenced. *F The fourth gene defined by transcript K was later shown to be fs(1)otu. *F None of the genes defined in Barbara Wakimoto's EMS screen described in the 82 *F Yearbook have been followed up by us, although they were sent to several other *F labs. Best check with her on this one. *F I hope you're having a nice summer. *F Allan *F *F To: spradling@mail1.ciwemb.edu *F Subject: Re: helping flybase! *F Cc: rd120@gen.cam.ac.uk *F *F *F Hi Allan, *F You wrote *F >The transcripts define 3 new presumed low abundance chorion protein genes: *F 'Presumed low abundance', not 'presumed chorion protein' genes, right? *F I must give these A, B/C and D/E genes name(s) as single letter *F transcript names won't really do for a variety of reasons. If you do *F know they are chorion protein genes then I can name them accordingly, *F as Cp7Fa, Cp7Fb and Cp7Fc. *F If you don't know that they are chorion protein genes they will get *F (less beautiful) anon-7Fa, b, and c names, unless you suggest *F something nicer. *F many thanks for your help *F Rachel *F *F From spradling@mail1.ciwemb.edu Fri Aug 11 14:29:25 1995 *F Date: 11 Aug 1995 09:29:42 +0000 *F From: 'Allan Spradling' *F Subject: Re: helping flybase! *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 1753 *F *F Reply to: RE>>helping flybase! *F *F Since they reside in an amplified gene cluster and are detectably expressed *F only during the time of chorion formation, I would consider them chorion *F proteins. Leave out the 'low abundance'. Cp7Fa etc., is fine with me. The *F correct reference for these is Parks, Wakimoto and Spradling, 1986, Dev. Biol. *F 117: 294-305. The EMBO J. paper just cites this earlier work in fig. 1. # *U FBrf0082769 *a Welte *b M. *t 1995.8.7 *T personal communication to FlyBase *u *F From mwelte@watson.princeton.edu Mon Aug 7 05:01:33 1995 *F Date: Sun, 06 Aug 1995 23:59:33 EDT *F From: Michael Welte *F To: ma11@gen.cam.ac.uk *F Cc: mwelte@watson.princeton.edu *F Subject: RE: help FlyBase please *F Content-Length: 251 *F *F Dear Dr. Ashburner, *F *F Marya Postner, a former graduate student in Eric Wieschaus' lab, has indeed *F mapped halo to 22A1-A2. I am currently working on mapping it molecularly. *F Is this message enough for you to update Flybase? *F *F Sincerely, *F Michael Welte # *U FBrf0082790 *a Wohlwill *b A. *t 1995.8.22 *T personal communication to FlyBase *u *F From U55850@uicvm.cc.uic.edu Tue Aug 22 23:56:20 1995 *F Date: Tue, 22 Aug 1995 17:45:07 -0500 (CDT) *F From: ARTHUR WOHLWILL *F Subject: errors in cytology *F To: flybase@morgan.harvard.edu *F Content-Transfer-Encoding: 7BIT *F Content-Length: 391 *F Flybase project members: It has come to my attention that there are *F some errors in the cytology of some of the deletions that I recovered in *F the 63 region. The following are shown as the correct cytology *F Df(3L)HR370 63A1-D1 Df(3L)HR232 63C1-D3 Df(3L)HR277 63C1-64B12 *F Df(3L)HR298 63B6-64A6. The cytology listed in the 1992 Redbook (under *F l(3)63)is correct. Mea Culpa, Arthur Wohlwill *F *F From rd120@gen.cam.ac.uk Wed Aug 23 11:45:55 1995 *F From: Rachel Drysdale (Genetics) *F Date: Wed, 23 Aug 95 11:45:07 BST *F To: U55850 < *F Subject: Re: errors in cytology *F Cc: flybase@morgan.harvard.edu *F Content-Length: 1218 *F Hi Arthur, *F Given that there has been some confusion about the correct cytology for *F the Df(3L)HR chromosomes in your message to FlyBase, please could you *F indicate which of the alternatives are correct for each Df. *F Lindsley and Zimm, | Wohlwill and Bonner *F 1992, page 382 | Genetics 128: 763--775 *F \---------------------|------------------------ *F Df(3L)HR370 63A1;63D1 | 63A1;63D10 *F Df(3L)HR232 63C1;63D3 | 63C1;63D1 *F Df(3L)HR277 63B12-C1;64B12 | 63B6;64B12 *F Df(3L)HR298 63B6;64A6 | 63B1;64A6 *F Many thanks! *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From ADwohlwi@uicvm.cc.uic.edu Wed Aug 23 16:13:39 1995 *F X-Sender: ADwohlwi@POP3SERV4.cc.uic.edu *F X-Mailer: Windows Eudora Version 2.0.3 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 23 Aug 1995 10:04:09 -0500 *F To: rd120@gen.cam.ac.uk *F From: ADwohlwi@uic.edu (Arthur Wohlwill) *F Subject: errors in cytology *F Content-Length: 152 *F Dr. Drysdale: All of the enteries in Lindsley and Zimm are correct whereas *F the Genetics paper contains some errors. of the Sorry for the confusion. *F From ADwohlwi@uicvm.cc.uic.edu Wed Aug 23 16:33:49 1995 *F X-Sender: ADwohlwi@POP3SERV4.cc.uic.edu *F X-Mailer: Windows Eudora Version 2.0.3 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 23 Aug 1995 10:23:29 -0500 *F To: ma11@gen.cam.ac.uk *F From: ADwohlwi@uic.edu (Arthur Wohlwill) *F Subject: cytology errors *F Content-Length: 731 *F Dr. Ashburner: I am sorry that I did not make myself clear. All of the *F entries in Lindsley and Zimm are correct---mistakes were made during *F preparation of the paper. Your information on HR370 is correct (it is *F 63A1-63D1 The Genetics paper says the right breakpoint is D10. *F Other corrections *F HR232---should be 63C1-D3 not 63C1-63D1 *F HR277---should be 63C1-64B12 not 63B6-64B12 *F HR298---should be 63B6-64A6 not 63B1-64A6 *F Finally pdp7 duplicates vn (according to Garcia-Bellido etal 1994 PNAS 91 *F 10222-10226) whereas your entry, citing the same reference, says that it *F deletes or disrupts vn. *F Rachel Drysdale also contacted me about these errors---I sent her a similar *F message. Again, I am sorry for the errors, Arthur Wohlwill # *U FBrf0082873 *a Bier *b E. *t 1995.10.13 *T personal communication to FlyBase *u *F Archived # *U FBrf0083626 *a MacDougall *b L.K. *c S.J. *d Leevers *t 1995.10.6 *T personal communication to FlyBase *u *F From sallyl@ludwig.ucl.ac.uk Fri Oct 6 11:07:41 1995 *F X-Sender: sallyl@kestrel.ludwig.ucl.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 6 Oct 1995 11:06:54 +0100 *F To: rd120@gen.cam.ac.uk *F From: sallyl@ludwig.ucl.ac.uk (Sally Leevers) *F Subject: PI3 Kinases *F Content-Length: 942 *F Dear Rachel, *F I have now spoken to Lindsay MacDougall about our nomenclature, *F and we would like to name and position the genes by personal communication *F as follows: *F PI3K-59F 59F Lindsay K MacDougall & Claude Linassier *F PI3K-68D 68D1-3 Lindsay K MacDougall *F PI3K-92D 92D1-4 Lindsay K MacDougall & Sally J Leevers *F I hope that this is ok, if not don't hesitate to get in touch. *F Thankyou! *F Sally Leevers *F Sally J. Leevers *F Ludwig Institute for Cancer Research *F 91 Riding House St *F London W1 8BT *F Tel/Voice mail: 0171 878 4067 *F Fax: 0171 878 4040 # *U FBrf0083627 *a MacDougall *b L.K. *t 1995.10.6 *T personal communication to FlyBase *u *F From sallyl@ludwig.ucl.ac.uk Fri Oct 6 11:07:41 1995 *F X-Sender: sallyl@kestrel.ludwig.ucl.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 6 Oct 1995 11:06:54 +0100 *F To: rd120@gen.cam.ac.uk *F From: sallyl@ludwig.ucl.ac.uk (Sally Leevers) *F Subject: PI3 Kinases *F Content-Length: 942 *F Dear Rachel, *F I have now spoken to Lindsay MacDougall about our nomenclature, *F and we would like to name and position the genes by personal communication *F as follows: *F PI3K-59F 59F Lindsay K MacDougall & Claude Linassier *F PI3K-68D 68D1-3 Lindsay K MacDougall *F PI3K-92D 92D1-4 Lindsay K MacDougall & Sally J Leevers *F I hope that this is ok, if not don't hesitate to get in touch. *F Thankyou! *F Sally Leevers *F Sally J. Leevers *F Ludwig Institute for Cancer Research *F 91 Riding House St *F London W1 8BT *F Tel/Voice mail: 0171 878 4067 *F Fax: 0171 878 4040 # *U FBrf0083628 *a MacDougall *b L.K. *c C. *d Linassier *t 1995.10.6 *T personal communication to FlyBase *u *F From sallyl@ludwig.ucl.ac.uk Fri Oct 6 11:07:41 1995 *F X-Sender: sallyl@kestrel.ludwig.ucl.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 6 Oct 1995 11:06:54 +0100 *F To: rd120@gen.cam.ac.uk *F From: sallyl@ludwig.ucl.ac.uk (Sally Leevers) *F Subject: PI3 Kinases *F Content-Length: 942 *F Dear Rachel, *F I have now spoken to Lindsay MacDougall about our nomenclature, *F and we would like to name and position the genes by personal communication *F as follows: *F PI3K-59F 59F Lindsay K MacDougall & Claude Linassier *F PI3K-68D 68D1-3 Lindsay K MacDougall *F PI3K-92D 92D1-4 Lindsay K MacDougall & Sally J Leevers *F I hope that this is ok, if not don't hesitate to get in touch. *F Thankyou! *F Sally Leevers *F Sally J. Leevers *F Ludwig Institute for Cancer Research *F 91 Riding House St *F London W1 8BT *F Tel/Voice mail: 0171 878 4067 *F Fax: 0171 878 4040 # *U FBrf0083629 *a Shirras *b A. *t 1995.9.20 *T personal communication to FlyBase *u *F Archived # *U FBrf0083630 *a Schafer *b U. *t 1995.9.19 *T personal communication to FlyBase *u *F Archived # *U FBrf0083632 *a Lorenz *b L. *t 1995.10.18 *T personal communication to FlyBase *u *F From LORENZ@rascal.med.harvard.edu Wed Oct 18 16:28:51 1995 *F Date: Wed, 18 Oct 1995 11:17:51 -0400 (EDT) *F From: LORENZ@rascal.med.harvard.edu *F To: rd120@gen.cam.ac.uk *F Subject: RE: hel *F Content-Length: 344 *F Dear Rachel, thank you for your note regarding our helicase, hel. *F I believe I spoke to M. Semeriva after I spoke at the Drosophila *F Conference here last spring. He did tell me they cloned another *F helicase using the 1A122 enhancer trap, which Norbert (Perrimon) *F sent to them. Our helicases are different--ours is at 25E. *F Regards, Lori Lorenz # *U FBrf0083633 *a Costa *b M. *t 1995.10.30 *T personal communication to FlyBase *u *F FAX: 00-1-206-667-6522 *F Dear Michael, *F I have a question about an allele of fog you used in your Cell paper: *F \*x FBrf0068458 == Costa et al., 1994, Cell 76(6): 1075--1089 *F You call it fog[RA67]. *F Is it possibly the same chromosome as Df(1)RA67, from the Umea stock list? *F >U-#18350 Df(1)RA67 / FM7, y[31d] sc[8] w[a] sn[X2] v[Of] g[4] B[1] / y[+] *F > Ymal[106] *F >Breakpts: ?; 20B-D *F >Comments: *F >Center: Umea Stock Center Contact: Karin Ekstrom karin.ekstrom@genetik.umu.se *F Many thanks for your help, *F best wishes, *F Rachel. *F From mcosta@fred.fhcrc.org Mon Oct 30 23:01:50 1995 *F Date: Mon, 30 Oct 95 14:56:28 PST *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: rd120@gen.cam.ac.uk *F From: mcosta@fred.fhcrc.org *F Subject: fog[RA67] *F Content-Length: 1631 *F Dear Rachel, *F The RA67 allele of fog mentioned in my Cell paper is at least derived from *F the the stock called Df(1)RA67 and quite possibly identical to it. When I *F tested the Df(1)RA67 strain that Eric Wieschaus had in the lab, I found *F that it complemented all mutations tested on both the proximal and distal *F side of fog. Therefore, I started calling it fog[RA67] since there was no *F genetic evidence that our allele was a deficiency. I never checked *F cytologically for a deletion in this strain and never found any molecular *F evidence of a deletion. Len Kelly has also found that his stock labelled *F Df(1)RA67 also behaves genetically as a simple fog mutation, and it is not *F clear whether Eric got his allele from Len or the Umea stock center. In *F any case, either the original complementation tests were done wrong for *F this strain (as I suspect), or the strain that Eric and Len have has *F changed from a deficieny to a fog allele over time. Obviously, if you plan *F to use this strain you should do the complementation tests on the stock you *F have. *F Feel free to send an e-mail message if you need further info. *F Mike *F Mike Costa *F 1100 Fairview Ave. N. *F Fred Hutchinson Cancer Research Center *F Dept. of Basic Sciences, A3-013 *F Seattle, WA 98109 *F USA *F phone:(206) 667-4085 *F FAX: (206) 667-3650 # *U FBrf0083634 *a Eckstrom *b K. *t 1995.11.2 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Nov 1 09:35:39 1995 *F From: Rachel Drysdale (Genetics) *F Date: Wed, 1 Nov 95 09:35:34 GMT *F To: karin.ekstrom@genetik.umu.se *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 1872 *F Hi Karen, *F As you know, we are doing some work on your stock list for FlyBase, and I *F have a question about the Df(1)RA67 stock: *F U-#18350 Df(1)RA67 / FM7, y[31d] sc[8] w[a] sn[X2] v[Of] g[4] B[1] / y[+] *F Ymal[106] *F Breakpts: ?; 20B-D *F Comments: *F Center: Umea Stock Center Contact: Karin Ekstrom karin.ekstrom@genetik.umu.se *F Do you know where the breakpoint data 'Breakpts: ?; 20B-D' came from, or *F what it might mean? *F I ask because Michael Costa, then of the Weischaus lab, has published an *F allele they call fog[RA67] and when asked about whether it may be *F Df(1)RA67, he said *F >The RA67 allele of fog mentioned in my Cell paper is at least derived from *F >the the stock called Df(1)RA67 and quite possibly identical to it. When I *F >tested the Df(1)RA67 strain that Eric Wieschaus had in the lab, I found *F >that it complemented all mutations tested on both the proximal and distal *F >side of fog. Therefore, I started calling it fog[RA67] since there was no *F >genetic evidence that our allele was a deficiency. I never checked *F >cytologically for a deletion in this strain and never found any molecular *F >evidence of a deletion. Len Kelly has also found that his stock labelled *F >Df(1)RA67 also behaves genetically as a simple fog mutation, and it is not *F >clear whether Eric got his allele from Len or the Umea stock center. *F So you see it is not QUITE clear that they are the same thing, and we would *F like to be sure! *F Many thanks for your help, *F best wishes, *F Rachel *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Karin.Ekstrom@genetik.umu.se Thu Nov 2 11:29:46 1995 *F Date: Thu, 2 Nov 1995 12:24:43 +0100 *F X-Sender: Karin.Ekstrom@genetik.umu.se *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: rd120@gen.cam.ac.uk *F From: Karin.Ekstrom@genetik.umu.se v (Karin Ekstr\vm) *F Subject: *F X-Mailer: *F Content-Length: 424 *F Dear Rachel, *F About stock 18350.Df(1)RA67/ FM7, we received that stock *F from George Miklos Canberra October -87, with the *F following information Df; 114[-]-EA41[-]. *F I did send a copy to Len Kelly in November-88. He told me *F that he was already holding a copy of the strain but it was *F not behaving genetically as the original Df(1)RA67. That is *F all information I have. We have never been testing the stock. *F Best wishes, *F Karin # *U FBrf0083707 *a Coulter *b D. *t 1996.1.23 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Mon Jan 22 14:25:52 1996 *F From: Aubrey de Grey (Genetics) *F Date: Mon, 22 Jan 96 14:19:46 GMT *F To: Coultede@wpogate.slu.edu *F Subject: L126 question *F Cc: ag24@gen.cam.ac.uk, eleanor@gen.cam.ac.uk *F Content-Length: 741 *F *F Dear Doug, *F *F In your very helpful exchange with Michael in October about sob and (in the *F end) bowl, there was a reference to T(Y;2)L126 breaking in bowl. We have *F only just noticed that this conflicts with our data on L126, which says it *F is a T(Y;2) with the autosomal break in division 40. So: *F *F - Does L126 have a third break, unknown to us, with 24 to 40 inverted, or *F - does it have just one autosomal break, in 24C, contra Lindsley & Zimm, or *F - is it a completely different aberration than the one in Lindsley & Zimm? *F *F If the last, then please give us your best information as the the other *F break or breaks -- and the new order, if it is multi-break. *F *F Many thanks in advance if you can sort this one out. *F *F Cheers, Aubrey de Grey *F FlyBase *F *F From Coultede@wpogate.slu.edu Tue Jan 23 21:22:45 1996 *F X-Mailer: Novell GroupWise 4.1 *F Date: Tue, 23 Jan 1996 15:15:40 -0600 *F From: Douglas E. Coulter *F To: ag24@gen.cam.ac.uk *F Subject: L126 question -Reply *F Content-Length: 987 *F *F Dear Aubrey- *F Thanks for your note. The break we have *F identified in bowl is almost certainly *F associated with T(Y;2)L26 (which does *F have an appropriate cytology) rather than *F L126. The stock sent to us was labeled *F T(Y;2)L126 and also cited as such in a *F relevant thesis chapter (J. Steven C. de *F Belle , 1990, 'Genetic analysis of foraging: *F a behavioral gene in Drosophila', York *F University) and paper (De Belle, *F Sokolowski, and Hilliker, 1993, Genome *F 36: 94-101). However, it's obvious that the *F stock in question must be L26 an not L126, *F given that Steve's thesis and paper also list a *F cytology appropriate to L26 (h25D; *F 24C2-D1, based on his reanalysis) and *F references Gatti and Pimpinelli (1983, *F Chromosoma 88: 349-373), which would *F not be appropriate for L126 as far as I can *F tell. Unfortunately, we have been guilty of *F propagating the error. I hope undoing our *F mistakes doesn't create too many headaches *F for you! *F With much appreciation for your diligent *F sleuthing - *F Doug Coulter # *U FBrf0083708 *a Kreber *b R. *t 1995 *T personal communication to FlyBase *u *F Personal communication from: Bob Kreber *F To: Bloomington Drosophila Stock Center *F Background: This is information on an unpublished duplication that is present *F in a stock contributed to the Bloomington collection by the Ganetzky lab. Bob *F Kreber was queried by the stock center about the identity of this duplication. *F Information communicated: Dp(2;2)BG is the name Michael Ashburner gave *F to a tandem duplication Bob Kreber found in a stock in the Ganetzky lab. It is *F duplicated for approximately 41BC to 42BC and covers Df(2R)M41A at the *F base of 2R. This duplication is superimposed on In(2LR)Gla. # *U FBrf0083709 *a Lambertsson *b A. *t 1995.12.20 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Thu Dec 14 17:38:13 1995 *F From: Michael Ashburner (Genetics) *F Date: Thu, 14 Dec 95 17:32:50 GMT *F To: andrew.lambertsson@bio.uio.no *F Subject: Help FlyBase *F Cc: ma11@gen.cam.ac.uk *F Content-Length: 874 *F *F Dear Andrew *F *F I have just seen yr EMBL sequence record X91853. *F *F Two questions *F *F 1. You call this M(2)32A, but there is no such gene in Lindsley and Zimm *F or FlyBase. Is this a new Minute or a new name for an old one. Should this *F be M(2)30A ? *F *F 2. You say this encodes ribosomal protein S13. The sequence is _almost_ *F the same as that McNabb and I reported in NAR *F (McNabb and Ashburner, 1993, Nucleic Acids Res. 21(10): 2523) *F and mapped to 29A. I assume this are in fact the same genes *F *F *F yr sequence *F *F GRMHAPGKGI SQSALPYRRT VPSWLKLNAD DVKEQIKKAG QEGSDSLQIG IILRDSHGVA *F QVRFVNGNKI LRIMKSVGLK PDIPEDLYHM IKKAVAIRKH LERNRKDKDG KFRLILVESR *F IHRLARYYKT KSVLPPNWKY ESSTASALVA *F *F McNabb and Ashburner *F *F *F GRMHAPGKGI SQSALPYRRT VPSWLKLNAD DVKEQIKKLG KKGLTPSKIG IILRDSHGVA *F QVRFVNGNKI LRIMKSVGLK PDIPEDLYHM IKKAVAIRKH LERNRKDKDG KFRLILVESR *F IHRLARYYKT KSVLPPNWKY ESSTASALVA *F *F *F Best wishes *F *F Michael *F *F ================================================================= *F >From andrew.lambertsson@bio.uio.no Wed Dec 20 10:10:19 1995 *F X-Sender: andrewl@darwin.uio.no *F X-Mailer: Windows Eudora Version 1.4.3 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 20 Dec 1995 11:04:19 +0100 *F To: m.ashburner@gen.cam.ac.uk *F From: andrew.lambertsson@bio.uio.no (Andrew Lambertsson) *F Subject: RPS13 *F Content-Length: 3931 *F *F Dear Michael, *F *F Thanks for your message. Almost two years ago I isolated two Minutes in a *F small scale P{lacW} element mutagenesis screen. The one on the second *F chromosome was mapped by both in situ hybridization and deficiency *F mapping(see below) to 32A. And, as you say, there is no previously described *F Minute there, so we assume it is a new one. The other Minute was mapped (as *F above) to 66D; again there is no reported Minute in that region. This one *F encodes RPL14; the rat RPL14 sequence will be published next year by Y-L *F Chan-Leung in Wool's lab. *F *F Deficiency mapping of P{lacW}M(2)32A1: The cytogenetic position of the *F P{lacW}M(2)32A1 mutation is defined genetically by overlapping deficiencies on *F chromosome arm 2L and duplications (Figure 1). The transpositions *F Tp(2;3)prd2.27.3 and Tp(2;3)dph27 consist of Df(2L)prd2.27.3 and Dp(2;3)prd2.27.3, *F and Df(2L)dph27 and Dp(2;3)dph27, respectively. Both Dp(2;3)prd2.27.3 and Dp(2;3)dph27 *F produce Minute phenotype when combined with P{lacW}M(2)32A1/Df(2L)prd2.27.3 and *F P{lacW}M(2)32A1/Df(2L) dph27, respectively, and produce wild type flies with *F P{lacW}M(2)32A1/+. Deficiencies Df(2L)Prl, Df(2L)J2 and Df(2L)J27 all *F complement P{lacW}M(2)32A1, producing Minute flies. These results confirm the in situ *F and recombination data, and we therefore conclude that P{lacW}M(2)32A1 is *F located in 32A (for exact breakpoints of the aberrations used here, see LINDSLEY and *F ZIMM 1992). To our knowledge no previously described Minute has been mapped to this *F region before (LINDSLEY and ZIMM 1992), and the present Minute mutation is therefore *F named P{lacW}M(2)32A1. *F *F *F To answer your second question I enclose a page from a manuscript (Title: A *F Novel Drosophila Minute Locus Encodes Ribosomal Protein S13) we have sent *F for publication: *F *F Sequence and primer extension analyses: To identify the gene disrupted by *F the P element insertion, one of the isolated cDNA clones and 1.9 kb genomic *F DNA was sequenced. The sequence of the cDNA clone was then compared with the *F sequences in the EMBL and GeneBank data libraries. This search revealed a very high *F level of identity (98.9% in a 564 bp overlap) to the Drosophila melanogaster RP17 cDNA *F (MCNABB and ASHBURNER 1993), and to RPS13 cDNA sequences from M. domestica *F (76%; Z.H. ZHOU and M. SYVANAN, unpublished), R. rattus (73%; SUZUKI et al. *F 1990), H. sapiens (71%; CHADENEAU et al. 1993), Z. mays (68%; JOANIN et al. 1993) *F and B. pahangi (67%; ELLENBERGER et al. 1989), among others. The very high level of *F identity between the RP17 cDNA and the one reported here, and the observations that both *F derive from single copy genes (see above; MCNABB and ASHBURNER 1993), suggest *F that these cDNAs originated from the same gene. When comparing the two cDNAs we *F detected a 68 bp long sequence at the 5' end of RP17 that is absent from our *F cDNA, and which has no counterpart in our genomic sequence (data not shown; accession *F numbers: RP17 Z19052 and RPS13 X91854). A closer look at the RP17 cDNA identified *F this 68 bp sequence as an inverted repeat of a sequence near the 3' end. The remaining *F differences are caused by five single nucleotide alterations. Two of these *F are found in the coding region and cause an alternative reading frame involving 10 amino *F acids. While our amino acid sequence in this region matches the other RPS13 *F sequences, the RP17 is dissimilar. It appears that the RP17 cDNA contains sequence *F artifacts, especially the 68 bp 5' end. Also, Rp17 was cytologically mapped to 29A on the *F polytene chromosome map, which is inconsistent with the genetic data we *F present here. Based on the high level of identity to RPS13 cDNAs from various species we *F conclude that our cDNA corresponds to the eukaryotic RpS13 gene. This cDNA will be *F referred to as RPS13. *F *F If you want I will send you the manuscript. I welcome any comments you may *F have on the enclosed data. *F *F Andrew *F ==================================================== *F >From ma11@gen.cam.ac.uk Wed Dec 20 10:56:33 1995 *F From: Michael Ashburner (Genetics) *F Date: Wed, 20 Dec 95 10:51:54 GMT *F To: m.ashburner@gen.cam.ac.uk, andrew.lambertsson@bio.uio.no *F Subject: Re: RPS13 *F Content-Length: 480 *F *F Andrew *F *F Thanks. It looks if we made something of a mess of 'RP17' ! *F I agree they must be the same gene, which, by the way, is now *F called RpS13 in FlyBase. Now you have identified a mutant *F phenotype there comes the problem of what to call the gene. *F The alternatives are RpS13 and M(2)32A. I would favor calling *F it RpS13, on the grounds that a 'functional' name is better *F than one based on phenotype. The mutant allele would then be *F RpS13[1] - by far the simplest name ! *F *F Michael # *U FBrf0083710 *a Paululat *b A. *t 1995.11.16 *T personal communication to FlyBase *u *F From paululat@papin.hrz.uni-marburg.de Thu Nov 16 16:19:30 1995 *F Date: Thu, 16 Nov 1995 17:14:21 +0100 *F From: 'paululat' *F To: m.ashburner@gen.cam.ac.uk *F Subject: to M. Ashburner *F Content-Length: 1751 *F *F Dear Dr. Ashburner, *F *F concerning your message: *F 'I have seen yr EMBL submission U28641, described as a trypsin. *F *F What relationship does this have to the existing 8 trypsin genes in *F Drosophila in region 47DF ? These are all called Try with a greek prefix. *F I think it would be better to call this gene Try also with a distinguishing *F prefix or suffix. Note that the use of 'D' for Drosophila is *F not acceptable for gene symbols in Drosophila (see Lindsley and Zimm, 1992 *F and the nomenclature document in the March 1995 supplement to TIGS). *F Is there any map data for this gene ?' *F *F Dear Dr. Ashburner, *F thank you very much for your note. U28641 shows a strong similarity to the *F Drosophila trypsin genes in region 47DF, but the strongest homology is visible *F with some trypsin related proteins from Anopheles gambiae and related species *F which were identified as trypsins (e.g. accession number P35035). Because the *F conserved motifs, typical for trypsins, are well present in U28641 it makes *F sense to use the trypsin nomenclature with a greek prefix. U28641 is very likely *F a trypsin, but we have only the data from the sequence and not from biochemical *F experiments. I have submitted a very short note to 'Gene' and I hope I will get *F an answer during the next weeks. Nevertheless I will change the name of U28641 *F in the database as well as in the manuscript, if it is accepted. *F On another note, the cytological localization of U28641 is region 29F/30A as it *F was shown by polytene in situs in our lab. *F *F Sincerely, *F Achim Paululat *F *F and best regards from Renate Renkawitz-Pohl too. *F *F Dr. Achim Paululat *F University Marburg *F Dep. Biology/Genetics *F Karl-von-Frisch Str. *F 35032 Marburg *F Fax.: 06421-288971 *F e-mail: paululat@mailer.uni-marburg.de *F *F ...... *F From ma11@gen.cam.ac.uk Thu Nov 16 16:59:47 1995 *F *F Dear Dr Paululat *F *F Thanks for your note. The obvious name would be *F Trypsin-29F with the symbol Try29F. *F *F The alternative would be to go for the next avaiable greek symbol, *F but FlyBase discourages the use of greek - it makes it *F harder for computer searches ! *F *F Let me know what you decide. *F *F Michael Ashburner # *U FBrf0083711 *a Roote *b J. *t 1996.1.2 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Jan 2 12:28:08 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 2 Jan 1996 12:22:36 +0100 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: help *F Content-Length: 800 *F *F >John any idea what Dp(2;2)BG is: *F *F You found a Dp(2;2) in the nap9/Gla stock which we assumed was on the nap9 *F chromosome. Later Ganetzky told us that he had also spotted it and it was *F on Gla (his Gla chromosome - don't know whether it's on all Gla). *F I named it BG after Barry Ganetsky. *F The cytology I have is 41AB;42B, but this could be out of date. *F John # *U FBrf0083712 *a Roote *b J. *t 1995.12.18 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Dec 18 12:02:12 1995 *F X-Sender: jr32@mole.bio.cam.ac.uk (Unverified) *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 18 Dec 1995 11:55:54 +0100 *F To: ma11 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: wb pc *F Content-Length: 3849 *F *F Michael *F *F The origins of the wb alleles we have in Cambridge: *F *F John *F *F \*j BMW22 \*j Bodmer & Walker \*k wb \*e EMS \*l Adh[uf3]rd[s]pr cn \*m l(2)br1, *F l(2)34Fb \# # *U FBrf0083713 *a Sun *b Y.H. *t 1995.11.8 *T personal communication to FlyBase *u *F From MBYHSUN@ccvax.sinica.edu.tw Wed Nov 8 01:18:14 1995 *F Date: Wed, 08 Nov 1995 09:15:36 +0800 *F From: MBYHSUN@ccvax.sinica.edu.tw *F Subject: Re: Help FlyBase *F To: ma11@gen.cam.ac.uk *F X-Vms-To: IN%'ma11@gen.cam.ac.uk' *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 834 *F *F Dear Michael: *F *F The Genbank submissions U32626 and U23185 were sequence of two cDNA clones *F isolated from an imaginal disc library (Nick Brown). We had an enhancer trap *F line with the P[lacW] mapped to 69C1-2. The genomic fragment flanking P[lacW] *F was isolated by plasmid rescue. It was then used to screen lambda genomic *F clones. One of the clones was used for the cDNA screen and the two cDNA clones *F were isolated. Unfortunately, we found that the lambda clones carry a 4 kb *F fragment which is repeated about 20 times in the genome and apprently not only *F on the third chromosome. So we don't really know the map location of the cDNA *F clones. Their encoded protein also showed no recognizable structural motifs or *F homology to other things. At this stage, we don't have anything to add to the *F sequence file. *F *F Best wishes. *F *F Henry Sun # *U FBrf0083714 *a Meister *b M. *c A. *d Braun *t 1995.10 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Marie Meister and Anne Braun, CNRS, Strasbourg *F Subject: lacZ expression patterns for P{} insertions at Bloomington *F Date: 5 Dec 1995 *F *F Background: *F Marie Meister and Anne Braun have provided a large set of late third instar (wandering stage) lacZ expression pattern data for Berkeley *F Drosophila Genome Project enhancer-trap P insertion lines held at the Bloomington Stock Center. It is here made available for curation *F by FlyBase. The following is excerpted from Marie Meister's letter of transmittal to the Bloomington Stock Center: *F *F "The staining has been done without fixation by direct dissection into a drop of X-Gal solution (0.2% X-gal, 3.5 mM K[[4]]Fe(CN)[[6]], *F 3.5 mM K[[3]]Fe(CN)[[6]], 1 mM MgCl[[2]], 150 mM NaCl, 10 mM Na[[2]]HPO[[4]], 10 mM NaH[[2]]PO[[4]] and 30% Ficoll). The *F omission of the fixation step allows a more rapid screen, but it may result in some inaccuracy in the brain area staining (the distinction *F between ring gland, imaginal discs and brain is somewhat difficult when the staining is strong and leaking). The indications concerning *F the gut staining, especially in the case of weak colorations, may be misleading because there are variable levels and patterns of *F endogenous galactosidase activities in these tissues, depending on the genetic background. The results are given in levels of staining *F intensity: *F *F ++ strong staining *F + intermediate *F (+) weak *F ((+)) very weak *F *F The following abbreviations are used: *F *F ad : antennal discs *F AG : anterior gut *F BR : brain *F ed : eye discs *F FB : fat body *F fir : foregut imaginal ring *F gc : gastric caeca *F GD : gonadal discs *F HG : hindgut *F hir : hindgut imaginal ring *F ID : imaginal discs *F Integ : integument *F MG : midgut *F mih : midgut imaginal histoblasts *F MT : Malpighian tubules *F Oeno : oenocytes *F Oes : oesophagus *F patt : staining which exhibits a pattern *F pg : prothoracic gland *F pv : proventriculus *F RG : ring gland *F SG : salivary gland *F Trach : trachaea " *F *F The cv used in adding these data to the Bloomington stock list in some cases differed from that used by Marie (changes have been cleared with *F Marie). Differences follow: *F *F anterior gut --> esophagus, proventriculus *F eye discs --> eye of eye-antennal disc *F gastric caeca --> gastric caecum *F gonadal discs --> gonad *F imaginal discs --> imaginal disc *F integument --> cuticle, somatic muscle, epidermis *F midgut imaginal histoblasts --> midgut imaginal island *F Malpighian tubules --> Malpighian tubule *F oenocytes --> oenocyte *F oesophagus --> esophagus *F patt --> pat (this to save space) *F trachaea --> trachea *F *F The following list contains the Meister-Braun data for each stock: *F *F 'P1660','larva:' *F 'P1777','larva:' *F 'P1763','larva: brain ((+' *F 'P2366','larva: brain ((+)), gonad (+)' *F 'P2031','larva: brain ((+)), imaginal disc ((+))' *F 'P2154','larva: brain ((+)), imaginal disc ((+))' *F 'P1063','larva: brain (+)' *F 'P1194','larva: brain (+)' *F 'P1275','larva: brain (+)' *F 'P1329','larva: brain (+)' *F 'P1338','larva: brain (+)' *F 'P1377','larva: brain (+)' *F 'P1515','larva: brain (+)' *F 'P1524','larva: brain (+)' *F 'P1545','larva: brain (+)' *F 'P1604','larva: brain (+)' *F 'P1628','larva: brain (+)' *F 'P1693','larva: brain (+)' *F 'P1773','larva: brain (+)' *F 'P2122','larva: brain (+)' *F 'P2325','larva: brain (+)' *F 'P2329','larva: brain (+)' *F 'P2339','larva: brain (+)' *F 'P2367','larva: brain (+)' *F 'P2371','larva: brain (+)' *F 'P941','larva: brain (+)' *F 'P1537','larva: brain (+), gonad (+)' *F 'P2361','larva: brain (+), gonad (+)' *F 'P2372','larva: brain (+), gonad (+)' *F 'P2373','larva: brain (+), gonad (+)' *F 'P1750','larva: brain (+), gonad (+)?' *F 'P1776','larva: brain (+), gonad +' *F 'P2076','larva: brain (+), imaginal disc ((+)), gonad (+)' *F 'P1053','larva: brain (+), imaginal disc (+)' *F 'P1192','larva: brain (+), imaginal disc (+)' *F 'P1193','larva: brain (+), imaginal disc (+)' *F 'P1209','larva: brain (+), imaginal disc (+)' *F 'P1383','larva: brain (+), imaginal disc (+)' *F 'P1388','larva: brain (+), imaginal disc (+)' *F 'P1448','larva: brain (+), imaginal disc (+)' *F 'P1613','larva: brain (+), imaginal disc (+)' *F 'P1616','larva: brain (+), imaginal disc (+)' *F 'P1644','larva: brain (+), imaginal disc (+)' *F 'P1646','larva: brain (+), imaginal disc (+)' *F 'P1758','larva: brain (+), imaginal disc (+)' *F 'P2060','larva: brain (+), imaginal disc (+)' *F 'P2351','larva: brain (+), imaginal disc (+)' *F 'P2358','larva: brain (+), imaginal disc (+)' *F 'P1215','larva: brain (+), imaginal disc (+), esophagus, proventriculus +, midgut +, hindgut (+), salivary gland +' *F 'P2341','larva: brain (+), imaginal disc (+), eye of eye-antennal disc +, gonad (+)' *F 'P2319','larva: brain (+), imaginal disc (+), fat body ((+))' *F 'P1364','larva: brain (+), imaginal disc (+), gonad (+)' *F 'P1389','larva: brain (+), imaginal disc (+), gonad (+)' *F 'P2053','larva: brain (+), imaginal disc (+), gonad (+)' *F 'P2323','larva: brain (+), imaginal disc (+), gonad (+)' *F 'P1761','larva: brain (+), imaginal disc (+), gonad +' *F 'P2330','larva: brain (+), imaginal disc (+), Malpighian tubule (+)' *F 'P1034','larva: brain (+), imaginal disc (+), Malpighian tubule (+), fat body +' *F 'P1172','larva: brain (+), imaginal disc (+), midgut (+)' *F 'P1495','larva: brain (+), imaginal disc (+), midgut (+)pat, hindgut +, salivary gland (+), trachea +' *F 'P2340','larva: brain (+), imaginal disc (+), salivary gland +' *F 'P1779','larva: brain (+), imaginal disc (+), trachea (+)?' *F 'P1349','larva: brain (+), imaginal disc (+)?' *F 'P984','larva: brain (+), imaginal disc (+)?' *F 'P1413','larva: brain (+), imaginal disc (+)?, gonad (+)' *F 'P1331','larva: brain (+), imaginal disc +' *F 'P1655','larva: brain (+), imaginal disc +' *F 'P1792','larva: brain (+), imaginal disc +' *F 'P2368','larva: brain (+), imaginal disc +' *F 'P978','larva: brain (+), imaginal disc +' *F 'P1396','larva: brain (+), imaginal disc ++' *F 'P1281','larva: brain (+), imaginal disc ++, esophagus, proventriculus +, midgut ++, hindgut +, Malpighian tubule +, fat body +, gonad (+)?, trachea +' *F 'P2370','larva: brain (+), imaginal disc ++, trachea (+)' *F 'P2352','larva: brain (+), imaginal disc +, esophagus, proventriculus (+), midgut (+), hindgut (+), Malpighian tubule +, salivary gland (+), fat body ((+)), gonad (+)' *F 'P1579','larva: brain (+), imaginal disc +, esophagus, proventriculus +, midgut (+), hindgut +, salivary gland ((+)), fat body (+), cuticle, somatic muscle, epidermis +' *F 'P1612','larva: brain (+), imaginal disc +, fat body ((+))' *F 'P1341','larva: brain (+), imaginal disc +, gonad (+)' *F 'P1484','larva: brain (+), imaginal disc +, gonad (+)' *F 'P2109','larva: brain (+), imaginal disc +, gonad (+)' *F 'P1401','larva: brain (+), imaginal disc +, Malpighian tubule +, salivary gland +' *F 'P1795','larva: brain (+), imaginal disc +, salivary gland +, fat body (+), trachea (+)' *F 'P1183','larva: brain (+), midgut (+)' *F 'P1497','larva: brain (+), midgut (+)' *F 'P1463','larva: brain (+), proventriculus (+)' *F 'P2042','larva: brain (+), proventriculus (+), midgut (+)pat' *F 'P1556','larva: brain (+), ring gland (+), esophagus, proventriculus (+), midgut ++, hindgut +, Malpighian tubule (+), salivary gland ++, fat body ++, oenocyte +' *F 'P1753','larva: brain (+), ring gland ++, imaginal disc ++, hindgut +, trachea +' *F 'P1258','larva: brain (+), ring gland +, midgut (+), Malpighian tubule (+), trachea +' *F 'P1295','larva: brain (+), ring gland +?, imaginal disc ++, esophagus, proventriculus ++, midgut ++, hindgut ++, Malpighian tubule +, salivary gland ++, fat body +' *F 'P1705','larva: brain (+), ring gland, prothoracic gland +, imaginal disc (+), Malpighian tubule (+), salivary gland (+), gonad (+), trachea (+)' *F 'P2112','larva: brain (+), salivary gland (+)' *F 'P866','larva: brain +, imaginal disc (+), fat body (+), trachea (+)' *F 'P1741','larva: brain +, imaginal disc (+), hindgut (+), cuticle, somatic muscle, epidermis +' *F 'P1781','larva: brain +, imaginal disc (+), midgut (+), salivary gland (+)' *F 'P1049','larva: brain +, imaginal disc +' *F 'P1728','larva: brain +, imaginal disc +' *F 'P937','larva: brain +, imaginal disc ++, oenocyte +, trachea +' *F 'P2380','larva: brain +, imaginal disc ++, proventriculus +, midgut (+)pat, Malpighian tubule +, salivary gland +, trachea +' *F 'P1039','larva: brain +, imaginal disc +, esophagus, proventriculus (+), midgut +, hindgut +, Malpighian tubule (+), salivary gland +, fat body (+), gonad +, cuticle, somatic muscle, epidermis +' *F 'P1201','larva: brain +, imaginal disc +, esophagus, proventriculus +, midgut +, hindgut +, Malpighian tubule +, fat body (+), trachea +' *F 'P802','larva: brain +, imaginal disc +, esophagus, proventriculus +, salivary gland +, trachea +' *F 'P1691','larva: brain +, imaginal disc +, fat body ((+))' *F 'P1179','larva: brain +, imaginal disc +, fat body +' *F 'P1706','larva: brain +, imaginal disc +, gonad (+)' *F 'P1204','larva: brain +, imaginal disc +, gonad +' *F 'P1114','larva: brain +, imaginal disc +, midgut +' *F 'P936','larva: brain +, imaginal disc +, midgut +, salivary gland +' *F 'P1744','larva: brain +, midgut (+)pat' *F 'P1734','larva: brain +, midgut +' *F 'P1335','larva: brain +, ring gland ((+)), imaginal disc (+)' *F 'P2360','larva: brain +, ring gland ++, imaginal disc (+), trachea +' *F 'P2377','larva: brain +, ring gland ++, imaginal disc +, midgut (+)pat, hindgut (+)pat, fat body ++' *F 'P2335','larva: brain +, ring gland +, imaginal disc (+), esophagus, proventriculus (+), midgut +, hindgut (+), Malpighian tubule (+), salivary gland ((+))' *F 'P1765','larva: brain +, ring gland +, imaginal disc ++, esophagus, proventriculus +, midgut +, hindgut ++, Malpighian tubule ++, salivary gland ++, fat body +, gonad +, cuticle, somatic muscle,' *F 'P2336','larva: brain +pat' *F 'P2150','larva: cuticle, somatic muscle, epidermis (+)' *F 'P943','larva: cuticle, somatic muscle, epidermis (+)?' *F 'P949','larva: cuticle, somatic muscle, epidermis (+)?' *F 'P1037','larva: cuticle, somatic muscle, epidermis +' *F 'P2062','larva: cuticle, somatic muscle, epidermis +' *F 'P2132','larva: esophagus, proventriculus (+), gonad (+)' *F 'P1483','larva: esophagus, proventriculus (+), midgut (+)' *F 'P1333','larva: esophagus, proventriculus (+), midgut (+), hindgut (+)' *F 'P2039','larva: esophagus, proventriculus (+), midgut (+), hindgut (+), salivary gland (+)' *F 'P1411','larva: esophagus, proventriculus (+), midgut (+), Malpighian tubule (+)' *F 'P2135','larva: esophagus, proventriculus (+), midgut (+)pat' *F 'P1065','larva: esophagus, proventriculus (+), midgut ++, hindgut ++, Malpighian tubule ++' *F 'P2148','larva: esophagus, proventriculus (+), midgut +, hindgut (+), Malpighian tubule (+), salivary gland (+), fat body ((+))' *F 'P2061','larva: esophagus, proventriculus (+)pat, midgut (+)pat, gonad (+)' *F 'P1381','larva: esophagus, proventriculus ++, midgut ++, hindgut ++, Malpighian tubule ++, fat body (+), trachea (+)' *F 'P2128','larva: esophagus, proventriculus ++, midgut ++, hindgut +, Malpighian tubule ++, salivary gland +, gonad +' *F 'P2058','larva: esophagus, proventriculus +, midgut (+)pat, salivary gland (+)' *F 'P1030','larva: esophagus, proventriculus +, midgut +, hindgut +, Malpighian tubule +, salivary gland +, cuticle, somatic muscle, epidermis +, trachea +' *F 'P2040','larva: esophagus, proventriculus +, midgut +, hindgut +, Malpighian tubule +, salivary gland +, fat body ++' *F 'P1062','larva: esophagus, proventriculus +, midgut +, hindgut +, salivary gland +, fat body (+)' *F 'P2106','larva: esophagus, proventriculus +, midgut +, Malpighian tubule (+), salivary gland +, fat body +' *F 'P1572','larva: esophagus, proventriculus +, midgut +, Malpighian tubule +' *F 'P2079','larva: eye of eye-antennal disc (+)' *F 'P2063','larva: eye-antennal disc (+)' *F 'P2072','larva: eye-antennal disc +' *F 'P2073','larva: fat body ((+))' *F 'P1046','larva: fat body (+)' *F 'P1055','larva: fat body (+)' *F 'P1518','larva: fat body (+)' *F 'P1664','larva: fat body (+)' *F 'P1722','larva: fat body (+)' *F 'P1729','larva: fat body (+)' *F 'P1760','larva: fat body (+)' *F 'P2130','larva: fat body (+)' *F 'P1452','larva: fat body ++' *F 'P975','larva: fat body ++' *F 'P1200','larva: foregut imaginal ring +, hindgut, hindgut imaginal ring +' *F 'P2322','larva: foregut imaginal ring +, midgut (+)' *F 'P1111','larva: foregut imaginal ring +, midgut (+), Malpighian tubule (+)pat' *F 'P2101','larva: gastric caecum (+), midgut (+), salivary gland +, fat body (+)' *F 'P1490','larva: gastric caecum (+), midgut +, fat body +' *F 'P1409','larva: gastric caecum +, Malpighian tubule +' *F 'P1697','larva: gastric caecum +, midgut (+)pat, hindgut +, Malpighian tubule (+)' *F 'P2142','larva: gastric caecum +, midgut (+)pat, Malpighian tubule (+)pat, salivary gland (+)' *F 'P1606','larva: gastric caecum +, midgut +, hindgut (+), Malpighian tubule +' *F 'P2357','larva: gastric caecum +, midgut +, hindgut +, cuticle, somatic muscle, epidermis +' *F 'P2052','larva: gastric caecum, proventriculus (+), midgut +, Malpighian tubule (+), salivary gland +, fat body (+), trachea (+)' *F 'P2144','larva: gastric caecum, proventriculus ++, midgut +, hindgut (+), salivary gland +, fat body (+)' *F 'P2069','larva: gastric caecum, proventriculus +, midgut +, Malpighian tubule +, salivary gland (+), fat body +' *F 'P2091','larva: gonad ((+))' *F 'P1371','larva: gonad (+)' *F 'P1498','larva: gonad (+)' *F 'P1504','larva: gonad (+)' *F 'P1768','larva: gonad (+)' *F 'P2071','larva: gonad (+)' *F 'P2077','larva: gonad (+)' *F 'P2143','larva: gonad (+)' *F 'P1764','larva: gonad (+)?' *F 'P2126','larva: gonad (+)?' *F 'P1617','larva: gonad +' *F 'P1772','larva: gonad +' *F 'P938','larva: gonad ++, trachea (+)' *F 'P2326','larva: hindgut ((+))' *F 'P1493','larva: hindgut (+)' *F 'P2137','larva: hindgut (+)' *F 'P2354','larva: hindgut (+)' *F 'P1582','larva: hindgut (+), fat body (+)' *F 'P1774','larva: hindgut (+), gonad (+)' *F 'P2344','larva: hindgut (+), gonad (+)' *F 'P2151','larva: hindgut (+), Malpighian tubule +, salivary gland (+), cuticle, somatic muscle, epidermis +, trachea (+)' *F 'P1522','larva: hindgut (+), trachea (+)' *F 'P1076','larva: hindgut (+), trachea +' *F 'P1742','larva: hindgut (+)?, salivary gland +' *F 'P2043','larva: hindgut (+)pat' *F 'P1369','larva: hindgut +' *F 'P1690','larva: hindgut +' *F 'P1648','larva: hindgut +, Malpighian tubule (+)' *F 'P1642','larva: hindgut +pat' *F 'P1330','larva: hindgut imaginal ring +' *F 'P1543','larva: imaginal disc ((+))' *F 'P1647','larva: imaginal disc ((+))' *F 'P1679','larva: imaginal disc ((+))' *F 'P2165','larva: imaginal disc ((+)), Malpighian tubule (+)' *F 'P1585','larva: imaginal disc ((+)), midgut (+)pat' *F 'P1787','larva: imaginal disc ((+)), trachea (+)' *F 'P1051','larva: imaginal disc (+)' *F 'P1177','larva: imaginal disc (+)' *F 'P1339','larva: imaginal disc (+)' *F 'P1343','larva: imaginal disc (+)' *F 'P1350','larva: imaginal disc (+)' *F 'P1352','larva: imaginal disc (+)' *F 'P1382','larva: imaginal disc (+)' *F 'P1384','larva: imaginal disc (+)' *F 'P1392','larva: imaginal disc (+)' *F 'P1407','larva: imaginal disc (+)' *F 'P1482','larva: imaginal disc (+)' *F 'P1485','larva: imaginal disc (+)' *F 'P1487','larva: imaginal disc (+)' *F 'P1491','larva: imaginal disc (+)' *F 'P1544','larva: imaginal disc (+)' *F 'P1619','larva: imaginal disc (+)' *F 'P1625','larva: imaginal disc (+)' *F 'P1641','larva: imaginal disc (+)' *F 'P1668','larva: imaginal disc (+)' *F 'P1671','larva: imaginal disc (+)' *F 'P1677','larva: imaginal disc (+)' *F 'P1711','larva: imaginal disc (+)' *F 'P1717','larva: imaginal disc (+)' *F 'P1720','larva: imaginal disc (+)' *F 'P1769','larva: imaginal disc (+)' *F 'P2045','larva: imaginal disc (+)' *F 'P2048','larva: imaginal disc (+)' *F 'P2055','larva: imaginal disc (+)' *F 'P2066','larva: imaginal disc (+)' *F 'P2075','larva: imaginal disc (+)' *F 'P2078','larva: imaginal disc (+)' *F 'P2081','larva: imaginal disc (+)' *F 'P2095','larva: imaginal disc (+)' *F 'P2111','larva: imaginal disc (+)' *F 'P2119','larva: imaginal disc (+)' *F 'P2167','larva: imaginal disc (+)' *F 'P2315','larva: imaginal disc (+)' *F 'P2338','larva: imaginal disc (+)' *F 'P2355','larva: imaginal disc (+)' *F 'P2369','larva: imaginal disc (+)' *F 'P827','larva: imaginal disc (+)' *F 'P1670','larva: imaginal disc (+), esophagus, proventriculus (+), midgut +pat, Malpighian tubule +pat' *F 'P1796','larva: imaginal disc (+), esphagous +, midgut +pat, hindgut +pat, Malpighian tubule ((+))pat, salivary gland ++, cuticle, somatic muscle, epidermis +, trachea ++' *F 'P2363','larva: imaginal disc (+), eye of eye-antennal disc ++' *F 'P1565','larva: imaginal disc (+), fat body ((+))' *F 'P2356','larva: imaginal disc (+), fat body ((+)), oenocyte (+)?' *F 'P935','larva: imaginal disc (+), fat body (+)' *F 'P2088','larva: imaginal disc (+), gastric caecum (+), midgut (+), salivary gland (+)' *F 'P1360','larva: imaginal disc (+), gastric caecum +, midgut +, hindgut (+), fat body (+)' *F 'P2056','larva: imaginal disc (+), gastric caecum, proventriculus +, midgut +, salivary gland (+)' *F 'P1345','larva: imaginal disc (+), gonad (+)' *F 'P1410','larva: imaginal disc (+), gonad (+)' *F 'P1514','larva: imaginal disc (+), gonad (+)' *F 'P1600','larva: imaginal disc (+), gonad (+)' *F 'P1603','larva: imaginal disc (+), gonad (+)' *F 'P1754','larva: imaginal disc (+), gonad (+)' *F 'P2155','larva: imaginal disc (+), gonad (+)' *F 'P2332','larva: imaginal disc (+), gonad (+)' *F 'P1632','larva: imaginal disc (+), gonad +' *F 'P1701','larva: imaginal disc (+), gonad +' *F 'P1778','larva: imaginal disc (+), gonad +' *F 'P1672','larva: imaginal disc (+), hindgut ((+))' *F 'P2067','larva: imaginal disc (+), hindgut (+)' *F 'P1713','larva: imaginal disc (+), hindgut (+), gonad (+)' *F 'P1532','larva: imaginal disc (+), hindgut +' *F 'P1568','larva: imaginal disc (+), hindgut +' *F 'P1451','larva: imaginal disc (+), hindgut +, Malpighian tubule (+), gonad (+)' *F 'P1724','larva: imaginal disc (+), Malpighian tubule (+)pat' *F 'P1106','larva: imaginal disc (+), Malpighian tubule +, fat body +' *F 'P2375','larva: imaginal disc (+), midgut ((+)), gonad (+)' *F 'P1521','larva: imaginal disc (+), midgut (+)' *F 'P1073','larva: imaginal disc (+), midgut +' *F 'P1570','larva: imaginal disc (+), midgut ++, Malpighian tubule (+), fat body +' *F 'P1479','larva: imaginal disc (+), midgut +, fat body +' *F 'P1536','larva: imaginal disc (+), midgut +, gonad (+)' *F 'P1615','larva: imaginal disc (+), midgut +pat' *F 'P1386','larva: imaginal disc (+), midgut +pat, fat body (+)' *F 'P1340','larva: imaginal disc (+), oenocyte +' *F 'P1342','larva: imaginal disc (+), oenocyte +, trachea +' *F 'P1480','larva: imaginal disc (+), proventriculus (+), gonad +' *F 'P1731','larva: imaginal disc (+), proventriculus +' *F 'P1637','larva: imaginal disc (+), proventriculus +, midgut ((+)), fat body +, gonad (+)' *F 'P2348','larva: imaginal disc (+), salivary gland (+)' *F 'P2114','larva: imaginal disc (+), salivary gland (+), gonad (+)' *F 'P2133','larva: imaginal disc (+), salivary gland (+), gonad (+)' *F 'P1737','larva: imaginal disc (+), salivary gland ++' *F 'P1735','larva: imaginal disc (+), trachea ((+))' *F 'P1719','larva: imaginal disc (+), trachea (+)' *F 'P1390','larva: imaginal disc (+)?' *F 'P1502','larva: imaginal disc (+)?' *F 'P1736','larva: imaginal disc (+)?' *F 'P1759','larva: imaginal disc (+)?' *F 'P2166','larva: imaginal disc (+)?, midgut (+)pat, salivary gland (+), gonad (+)' *F 'P2333','larva: imaginal disc (+)?, salivary gland (+)' *F 'P2065','larva: imaginal disc (+)pat' *F 'P2115','larva: imaginal disc (+)pat' *F 'P2138','larva: imaginal disc (+)pat' *F 'P1244','larva: imaginal disc \*' *F 'P1176','larva: imaginal disc +' *F 'P1346','larva: imaginal disc +' *F 'P1359','larva: imaginal disc +' *F 'P1378','larva: imaginal disc +' *F 'P1379','larva: imaginal disc +' *F 'P1506','larva: imaginal disc +' *F 'P1554','larva: imaginal disc +' *F 'P1571','larva: imaginal disc +' *F 'P1575','larva: imaginal disc +' *F 'P1592','larva: imaginal disc +' *F 'P1633','larva: imaginal disc +' *F 'P1650','larva: imaginal disc +' *F 'P1654','larva: imaginal disc +' *F 'P1674','larva: imaginal disc +' *F 'P1676','larva: imaginal disc +' *F 'P1678','larva: imaginal disc +' *F 'P1684','larva: imaginal disc +' *F 'P1710','larva: imaginal disc +' *F 'P1733','larva: imaginal disc +' *F 'P2036','larva: imaginal disc +' *F 'P2374','larva: imaginal disc +' *F 'P1082','larva: imaginal disc ++' *F 'P1376','larva: imaginal disc ++' *F 'P1394','larva: imaginal disc ++' *F 'P1626','larva: imaginal disc ++' *F 'P1683','larva: imaginal disc ++' *F 'P2047','larva: imaginal disc ++' *F 'P981','larva: imaginal disc ++, cuticle, somatic muscle, epidermis (+)?' *F 'P1590','larva: imaginal disc ++, esophagus, proventriculus +, midgut +, Malpighian tubule +, fat body +' *F 'P1692','larva: imaginal disc ++, esophagus, proventriculus, gastric caecum +, midgut +, hindgut +, Malpighian tubule +, fat body +, cuticle, somatic muscle, epidermis +, trachea +' *F 'P1716','larva: imaginal disc ++, foregut imaginal ring +, hindgut, hindgut imaginal ring +, salivary gland ++, cuticle, somatic muscle, epidermis (+), trachea +' *F 'P1525','larva: imaginal disc ++, gonad ++' *F 'P2034','larva: imaginal disc ++, hindgut (+)pat, trachea (+)' *F 'P1629','larva: imaginal disc ++, hindgut imaginal ring +, trachea +' *F 'P1029','larva: imaginal disc ++, midgut +, trachea +' *F 'P1486','larva: imaginal disc ++, salivary gland (+)' *F 'P1651','larva: imaginal disc ++, trachea ++' *F 'P2379','larva: imaginal disc ++pat' *F 'P2041','larva: imaginal disc ++pat, hindgut (+)' *F 'P1718','larva: imaginal disc +, esophagus, proventriculus (+), midgut (+), hindgut (+), Malpighian tubule +pat, trachea +' *F 'P1564','larva: imaginal disc +, esophagus, proventriculus (+), midgut +, hindgut +, Malpighian tubule (+), salivary gland (+)' *F 'P1567','larva: imaginal disc +, esophagus, proventriculus ++, midgut ++, hindgut +, Malpighian tubule ++, salivary gland +, fat body +, gonad (+), trachea +' *F 'P1756','larva: imaginal disc +, esophagus, proventriculus +, midgut +, hindgut +, Malpighian tubule (+), fat body (+)' *F 'P2350','larva: imaginal disc +, esophagus, proventriculus +, midgut +, hindgut +, Malpighian tubule +, salivary gland +, fat body (+)' *F 'P1548','larva: imaginal disc +, esophagus, proventriculus +, midgut +, Malpighian tubule +, salivary gland (+), fat body +' *F 'P1405','larva: imaginal disc +, fat body (+)' *F 'P1562','larva: imaginal disc +, fat body (+)' *F 'P946','larva: imaginal disc +, fat body +, cuticle, somatic muscle, epidermis +' *F 'P1574','larva: imaginal disc +, foregut imaginal ring +, midgut imaginal island +, trachea (+)' *F 'P1681','larva: imaginal disc +, gastric caecum +, midgut (+), hindgut (+), Malpighian tubule (+), fat body +' *F 'P2378','larva: imaginal disc +, gastric caecum +, midgut (+), hindgut +, Malpighian tubule +, salivary gland (+), fat body ((+)), gonad (+)' *F 'P1783','larva: imaginal disc +, gonad (+)' *F 'P1040','larva: imaginal disc +, gonad +' *F 'P1782','larva: imaginal disc +, gonad +' *F 'P2074','larva: imaginal disc +, gonad +' *F 'P1511','larva: imaginal disc +, gonad +?' *F 'P1786','larva: imaginal disc +, hindgut (+), hindgut imaginal ring +, Malpighian tubule (+), trachea +' *F 'P1749','larva: imaginal disc +, hindgut (+), Malpighian tubule (+)pat' *F 'P1555','larva: imaginal disc +, hindgut (+), salivary gland +pat, cuticle, somatic muscle, epidermis +, trachea +' *F 'P1500','larva: imaginal disc +, hindgut (+), trachea (+)' *F 'P1785','larva: imaginal disc +, hindgut +, Malpighian tubule (+), salivary gland +, oenocyte (+)?' *F 'P1261','larva: imaginal disc +, hindgut imaginal ring +' *F 'P1698','larva: imaginal disc +, hindgut imaginal ring +, gonad (+)' *F 'P1662','larva: imaginal disc +, hindgut imaginal ring +, trachea +' *F 'P1542','larva: imaginal disc +, Malpighian tubule (+)' *F 'P1530','larva: imaginal disc +, Malpighian tubule (+), salivary gland (+)' *F 'P1762','larva: imaginal disc +, Malpighian tubule (+)?, gonad (+)' *F 'P1278','larva: imaginal disc +, Malpighian tubule +' *F 'P1569','larva: imaginal disc +, Malpighian tubule +pat' *F 'P1551','larva: imaginal disc +, midgut (+)' *F 'P1687','larva: imaginal disc +, midgut (+), Malpighian tubule (+), fat body (+)' *F 'P1373','larva: imaginal disc +, midgut (+), Malpighian tubule +' *F 'P1657','larva: imaginal disc +, midgut (+)pat' *F 'P1730','larva: imaginal disc +, midgut (+)pat, hindgut ++, Malpighian tubule (+), salivary gland +' *F 'P1494','larva: imaginal disc +, midgut (+)pat, trachea +' *F 'P1563','larva: imaginal disc +, midgut ++, fat body ++' *F 'P1609','larva: imaginal disc +, midgut +, gonad +' *F 'P1581','larva: imaginal disc +, midgut +, hindgut (+), Malpighian tubule +, fat body (+)' *F 'P1622','larva: imaginal disc +, midgut +, hindgut +, Malpighian tubule +, fat body (+)' *F 'P1370','larva: imaginal disc +, midgut +, Malpighian tubule +' *F 'P1336','larva: imaginal disc +, midgut +, Malpighian tubule ++, fat body (+)' *F 'P1594','larva: imaginal disc +, midgut +pat, Malpighian tubule (+), gonad +' *F 'P1663','larva: imaginal disc +, proventriculus +, salivary gland +, gonad (+)' *F 'P2134','larva: imaginal disc +, salivary gland +, fat body ((+))' *F 'P1507','larva: imaginal disc +, salivary gland +pat' *F 'P1292','larva: imaginal disc +, trachea (+)' *F 'P1185','larva: imaginal disc +pat' *F 'P1205','larva: imaginal disc +pat, foregut imaginal ring +, midgut ++, hindgut imaginal ring +, cuticle, somatic muscle, epidermis +' *F 'P2090','larva: Malpighian tubule ((+)), salivary gland (+), fat body (+)' *F 'P1614','larva: Malpighian tubule (+)' *F 'P2161','larva: Malpighian tubule (+)' *F 'P1725','larva: Malpighian tubule (+), fat body ++' *F 'P1605','larva: Malpighian tubule (+), salivary gland +' *F 'P1045','larva: Malpighian tubule (+)pat, oenocyte +' *F 'P1079','larva: midgut (+)' *F 'P1496','larva: midgut (+)' *F 'P1790','larva: midgut (+)' *F 'P1143','larva: midgut (+), cuticle, somatic muscle, epidermis (+)' *F 'P2068','larva: midgut (+), fat body (+)' *F 'P1740','larva: midgut (+), hindgut ((+))' *F 'P1513','larva: midgut (+), hindgut +, fat body (+)' *F 'P1509','larva: midgut (+), Malpighian tubule +' *F 'P1277','larva: midgut (+), Malpighian tubule +, trachea (+)' *F 'P1757','larva: midgut (+), salivary gland (+), trachea (+)' *F 'P1745','larva: midgut (+)pat' *F 'P1789','larva: midgut (+)pat' *F 'P1793','larva: midgut (+)pat' *F 'P2129','larva: midgut (+)pat' *F 'P1337','larva: midgut (+)pat, hindgut (+)pat, Malpighian tubule (+)pat' *F 'P1636','larva: midgut (+)pat, Malpighian tubule (+)' *F 'P1669','larva: midgut (+)pat, Malpighian tubule (+), salivary gland +, fat body +' *F 'P962','larva: midgut +, fat body (+)' *F 'P1068','larva: midgut +, hindgut (+), Malpighian tubule (+)' *F 'P1478','larva: midgut +, hindgut +, Malpighian tubule +, salivary gland +, fat body +, trachea (+)' *F 'P1056','larva: midgut +, Malpighian tubule +' *F 'P1520','larva: midgut +, Malpighian tubule +, fat body +' *F 'P944','larva: midgut +, Malpighian tubule +, fat body +' *F 'P1527','larva: midgut +pat' *F 'P2160','larva: midgut +pat, hindgut (+), salivary gland (+)' *F 'P2146','larva: midgut +pat, hindgut +, Malpighian tubule +' *F 'P1003','larva: no staining' *F 'P1026','larva: no staining' *F 'P1035','larva: no staining' *F 'P1140','larva: no staining' *F 'P1181','larva: no staining' *F 'P1190','larva: no staining' *F 'P1198','larva: no staining' *F 'P1211','larva: no staining' *F 'P1213','larva: no staining' *F 'P1214','larva: no staining' *F 'P1257','larva: no staining' *F 'P1265','larva: no staining' *F 'P1271','larva: no staining' *F 'P1279','larva: no staining' *F 'P1294','larva: no staining' *F 'P1344','larva: no staining' *F 'P1348','larva: no staining' *F 'P1351','larva: no staining' *F 'P1353','larva: no staining' *F 'P1361','larva: no staining' *F 'P1362','larva: no staining' *F 'P1363','larva: no staining' *F 'P1365','larva: no staining' *F 'P1367','larva: no staining' *F 'P1374','larva: no staining' *F 'P1375','larva: no staining' *F 'P1380','larva: no staining' *F 'P1385','larva: no staining' *F 'P1387','larva: no staining' *F 'P1391','larva: no staining' *F 'P1393','larva: no staining' *F 'P1399','larva: no staining' *F 'P1400','larva: no staining' *F 'P1402','larva: no staining' *F 'P1404','larva: no staining' *F 'P1408','larva: no staining' *F 'P1412','larva: no staining' *F 'P1449','larva: no staining' *F 'P1469','larva: no staining' *F 'P1476','larva: no staining' *F 'P1488','larva: no staining' *F 'P1489','larva: no staining' *F 'P1501','larva: no staining' *F 'P1505','larva: no staining' *F 'P1508','larva: no staining' *F 'P1510','larva: no staining' *F 'P1512','larva: no staining' *F 'P1523','larva: no staining' *F 'P1526','larva: no staining' *F 'P1529','larva: no staining' *F 'P1531','larva: no staining' *F 'P1533','larva: no staining' *F 'P1534','larva: no staining' *F 'P1538','larva: no staining' *F 'P1539','larva: no staining' *F 'P1541','larva: no staining' *F 'P1552','larva: no staining' *F 'P1553','larva: no staining' *F 'P1557','larva: no staining' *F 'P1558','larva: no staining' *F 'P1560','larva: no staining' *F 'P1561','larva: no staining' *F 'P1566','larva: no staining' *F 'P1577','larva: no staining' *F 'P1578','larva: no staining' *F 'P1583','larva: no staining' *F 'P1584','larva: no staining' *F 'P1586','larva: no staining' *F 'P1589','larva: no staining' *F 'P1597','larva: no staining' *F 'P1598','larva: no staining' *F 'P1599','larva: no staining' *F 'P1602','larva: no staining' *F 'P1607','larva: no staining' *F 'P1611','larva: no staining' *F 'P1618','larva: no staining' *F 'P1620','larva: no staining' *F 'P1621','larva: no staining' *F 'P1630','larva: no staining' *F 'P1631','larva: no staining' *F 'P1634','larva: no staining' *F 'P1635','larva: no staining' *F 'P1638','larva: no staining' *F 'P1643','larva: no staining' *F 'P1645','larva: no staining' *F 'P1656','larva: no staining' *F 'P1658','larva: no staining' *F 'P1661','larva: no staining' *F 'P1666','larva: no staining' *F 'P1667','larva: no staining' *F 'P1675','larva: no staining' *F 'P1682','larva: no staining' *F 'P1686','larva: no staining' *F 'P1688','larva: no staining' *F 'P1689','larva: no staining' *F 'P1694','larva: no staining' *F 'P1695','larva: no staining' *F 'P1699','larva: no staining' *F 'P1700','larva: no staining' *F 'P1703','larva: no staining' *F 'P1704','larva: no staining' *F 'P1712','larva: no staining' *F 'P1714','larva: no staining' *F 'P1715','larva: no staining' *F 'P1721','larva: no staining' *F 'P1723','larva: no staining' *F 'P1726','larva: no staining' *F 'P1738','larva: no staining' *F 'P1743','larva: no staining' *F 'P1746','larva: no staining' *F 'P1748','larva: no staining' *F 'P1751','larva: no staining' *F 'P1752','larva: no staining' *F 'P1755','larva: no staining' *F 'P1766','larva: no staining' *F 'P1767','larva: no staining' *F 'P1784','larva: no staining' *F 'P1791','larva: no staining' *F 'P1794','larva: no staining' *F 'P2035','larva: no staining' *F 'P2037','larva: no staining' *F 'P2044','larva: no staining' *F 'P2057','larva: no staining' *F 'P2059','larva: no staining' *F 'P2070','larva: no staining' *F 'P2080','larva: no staining' *F 'P2082','larva: no staining' *F 'P2084','larva: no staining' *F 'P2085','larva: no staining' *F 'P2086','larva: no staining' *F 'P2087','larva: no staining' *F 'P2089','larva: no staining' *F 'P2092','larva: no staining' *F 'P2096','larva: no staining' *F 'P2097','larva: no staining' *F 'P2100','larva: no staining' *F 'P2104','larva: no staining' *F 'P2105','larva: no staining' *F 'P2107','larva: no staining' *F 'P2108','larva: no staining' *F 'P2110','larva: no staining' *F 'P2116','larva: no staining' *F 'P2117','larva: no staining' *F 'P2118','larva: no staining' *F 'P2123','larva: no staining' *F 'P2124','larva: no staining' *F 'P2136','larva: no staining' *F 'P2145','larva: no staining' *F 'P2152','larva: no staining' *F 'P2157','larva: no staining' *F 'P2159','larva: no staining' *F 'P2162','larva: no staining' *F 'P2164','larva: no staining' *F 'P2316','larva: no staining' *F 'P2320','larva: no staining' *F 'P2324','larva: no staining' *F 'P2331','larva: no staining' *F 'P2337','larva: no staining' *F 'P2342','larva: no staining' *F 'P2345','larva: no staining' *F 'P2346','larva: no staining' *F 'P2362','larva: no staining' *F 'P2381','larva: no staining' *F 'P942','larva: no staining' *F 'P976','larva: no staining' *F 'P979','larva: no staining' *F 'P989','larva: no staining' *F 'P999','larva: no staining' *F 'P2050','larva: proventriculus (+)' *F 'P2051','larva: proventriculus (+)' *F 'P2049','larva: proventriculus (+), fat body (+)' *F 'P1788','larva: proventriculus (+), salivary gland (+)' *F 'P1601','larva: proventriculus +, midgut (+), salivary gland +' *F 'P1775','larva: proventriculus +, midgut (+)pat, Malpighian tubule +' *F 'P1652','larva: proventriculus +, midgut +, hindgut (+), Malpighian tubule (+), fat body +, trachea (+)' *F 'P1528','larva: proventriculus, foregut imaginal ring +, midgut imaginal island +, hindgut +pat, hindgut imaginal ring +, cuticle, somatic muscle, epidermis +, trachea +' *F 'P1610','larva: ring gland (+), imaginal disc (+)' *F 'P2103','larva: ring gland (+), salivary gland (+), fat body ((+)), oenocyte (+), gonad (+)' *F 'P1702','larva: ring gl # *U FBrf0083715 *a Christensen *b A. *t 1996.1.19 *T personal communication to FlyBase *u *F From achristensen@crcvms.unl.edu Fri Jan 19 21:27:32 1996 *F Date: Fri, 19 Jan 1996 15:05:34 -0600 *F From: achristensen@crcvms.unl.edu (Alan C. Christensen, Ph.D.) *F Subject: Revised FB entry for Tpl *F To: flybase-updates@morgan.harvard.edu *F Mime-Version: 1.0 *F Content-Type: multipart/mixed; boundary='========================_64989128==_' *F Content-Transfer-Encoding: 7BIT *F Content-Length: 11959 *F Status: RO *F X-Lines: 292 *F *F *F *F --========================_64989128==_ *F Content-Type: text/plain; charset='us-ascii' *F *F Dear Colleagues, *F *F I have attached a revised version of the FB entry for Tpl. The revisions *F consist of correction of errors that date back to the DIS version of *F Lindsley and Zimm, as well as corrections from new data recently published *F (Dorer et al, Dec. 1995, Genetics 141(3): 1037-1042. The revision was *F constructed by downloading the current entry as text, and then editing it. *F I have noted lines that need to be added or changed from the current entry *F with a \*, lines that need to be deleted with a >, and lines that consist of *F my comments (not to be included in the entry, but to explain to you why a *F change is necessary) with a ~. If you have any questions or if my mailer *F does not attach the file properly to this message please let me know. *F *F Sincerely, *F Alan Christensen *F *F *F *F --========================_64989128==_ *F Content-Type: text/plain; name='Tpl'; charset='iso-8859-1' *F Content-Transfer-Encoding: quoted-printable *F Content-Disposition: attachment; filename='Tpl' *F *F ~Revised FB entry for Tpl. *F ~Please note: *F ~Lines beginning with a \* indicate additions or changes to the current entry *F ~Lines beginning with a ~ indicate comments from me that explain why somethi= *F ng *F ~should be changed or deleted *F ~Lines beginning with a > indicate lines in the current entry that should *F ~be deleted *F *F Gene symbol Tpl *F \*Full name Triplo-lethal *F =46lyBase ID number FBgn0003739 Graphic map *F Synonym(s) tpl *F Date 13 Oct 95 *F \*Genetic map position very close to Ki because deletions of Tpl *F \* also delete Ki (Roehrdanz and Lucchesi, 1980), *F \* and because no deletions of Ki were obtained *F \* presumably due to the close proximity of Tpl *F \* (Kaufman, 1978) *F *F Cytological map position *F Located in 83D5-E1 because included in Df(3R)Tpl4 =3D *F Df(3R)83D4-5;83E1-2 and Df(3R)Tpl7 =3D Df(3R)83D4-5;83E1-2 (Keppy *F and Denell, 1979). *F Revised cyto. map posn. 83E1--83E2 *F *F Phenotypic information *F Unique dosage-sensitive locus at 83D-E; first detected by *F segmental aneuploidy, using the Y-autosome translocations *F T(Y;3)L132 and Tp(Y;3)A109 (Lindsley, Sandler, Baker, Carpenter, *F Denell, Hall, Jacobs, Miklos, Davis, Gethmann, Hardy, Hessler, *F Miller, Nozawa, Parry and Gould-Somero, 1972). Lethal when *F present in either one (Tpl/Df) or three doses (Tpl/Dp) in an *F otherwise diploid individual. These individuals do not survive *F to the adult stage, but a few larvae with three doses of 83D-E *F develop to the third instar. The surviving larvae are also *F hyperploid for the X chromosome (as in 3X;2A metafemales); they *F can be produced in genotypes duplicated for 7C and 7D-E *F \* (Roehrdanz and Lucchesi, 1979; Roehrdanz and Lucchesi 1981). This *F \* interaction is due to the Isis locus (Dorer et al., 1993). Flies *F \* with a deficiency for 83D-E in one chromosome 3 and a duplication for *F the region in the other (two doses in all) are viable (Denell, *F \* 1976). Crosses of these Df/Dp flies to wild type *F \* fail to produce viable adults (Keppy and Denell, 1979). *F \* When wild-type flies *F \* are treated *F with EMS or gamma-rays (Keppy and Denell, 1979; Roehrdanz and *F \* Lucchesi, 1980), new deficiencies of Tpl and mutations in the tightly li= *F nked *F \* Su(Tpl) locus are obtained (Dorer et al., 1995). No function in measurin= *F g *F \* the X/A ratio has been observed in Tpl; Tpl-aneuploids are not rescued *F by mutations in *F \* the sex-determining genes Sxl and da (Christensen and *F Lucchesi, 1988). *F ~Delete the entire paragraph of Miscellaneous info, as it is incorrect *F >Miscellaneous info... *F *F Data from ref. FBrf0051978 *F Phenotypic information *F \* Hybrid dysgenesis has been used to mutate the Tpl locus, but no amorph= *F ic *F \* alleles were generated, suggesting Tpl may be a complex locus. *F ~Delete this sentence because it really refers to Su(Tpl) *F > A higher mutation rate with hybrid dysgenesis than with *F > radiation or chemicals suggests a peculiar genetic *F > organization of Tpl. *F *F Data from ref. FBrf0058587 *F Phenotypic information *F \* Increased dosage of the Is locus suppresses the triplo-lethal, but *F \* not the haplo-lethal phenotype of Tpl. Altered dosage of Is in *F the presence of two copies of Tpl has no obvious effects. Is *F probably does not directly repress Tpl expression, but rather *F acts downstream of the Tpl triplo-lethal phenotype. *F *F Data from ref. FBrf0066351 *F Phenotypic information *F Flies with three or one dose of Tpl die as late embryos or early *F first instar larvae. Lethality can be rescued by dosage of the *F \* Is locus, or mutations of the Su(Tpl) locus *F *F ~Delete this allele, because it is an allele of Su(Tpl), listed in that entr= *F y *F >Allele Tpl[J34] *F > FlyBase ID number FBal0030331 *F > *F > References *F > Dorer and Christensen, 1990, Genetics 125: 795--801 *F > [FBrf0051978] *F > *F > Data from ref. FBrf0051978 *F > Mutagen PM hybrid dysgenesis *F > Associated aberration(s) P{}Tpl[J34] *F > Phenotypic information Allele class: hypomorph *F *F ~Rename because this is NOT a revertant. That was an error in Lindsley and *F ~Zimm. I suggest keeping the name 10d77-6. It is most likely to have been *F ~a small deficiency,but it has unfortunately been lost, so we'll never know. *F \*Allele Tpl[10d77-6] *F Synonym(s) 10d77-6 *F FlyBase ID number FBal0016998 *F Mutagen ethyl methanesulfonate *F *F References *F Keppy and Denell, 1979, Genetics 91: 421--441 [FBrf0033180] *F *F ~Rename because this is NOT a revertant. That was an error in Lindsley and *F ~Zimm. I suggest keeping the name 18i77. It may be a small deficiency, *F ~so it will probably need to be renamed again, but it's better to wait until *F ~we know what it really is. *F \*Allele Tpl[18i77] *F Synonym(s) 18i77 *F FlyBase ID number FBal0016999 *F Mutagen formaldehyde *F *F References *F Keppy and Denell, 1979, Genetics 91: 421--441 [FBrf0033180] *F Dorer et al., 1993, Genetics 134(1): 243--249 [FBrf0058587] *F *F ~Delete this, and wait until we know what it really is *F > Data from ref. FBrf0058587 *F > Synonym(s) Df(3R)l8i77 *F *F ~Delete this allele for two reasons: (1) because it is an allele of Su(Tpl), *F ~listed in that entry, and (2) because it is NOT a revertant - that was an *F ~error in Lindsley and Zimm, and the renaming of it as Tpl[rv3] was *F ~inappropriate and incorrect. *F >Allele Tpl[rv3] *F > Synonym(s) tpl[10] *F > FlyBase ID number FBal0017000 *F > Mutagen ethyl methanesulfonate *F > *F > References *F > Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 *F > [FBrf0034851] *F > Dorer and Christensen, 1989, Genetics 122: 397--401 *F > [FBrf0049901] *F > Dorer and Christensen, 1990, Genetics 125: 795--801 *F > [FBrf0051978] *F > *F > Data from ref. FBrf0051978 *F > Molecular biology data *F > Insertion of an unidentified mobile element. *F > Mutagen insertion *F > Phenotypic information *F > Allele class: hypomorph *F > Phenotypic class: lethal | recessive *F > Site specific increase in recombination frequency and premeiotic *F > recombination in males. *F > Synonym(s) Tpl[10] *F *F ~Delete this allele for two reasons: (1) because it is an allele of Su(Tpl), *F ~listed in that entry, and (2) because it is NOT a revertant - that was an *F ~error in Lindsley and Zimm, and the renaming of it as Tpl[rv4] was *F ~inappropriate and incorrect. *F >Allele Tpl[rv4] *F > Synonym(s) tpl[17] *F > FlyBase ID number FBal0017001 *F > Mutagen ethyl methanesulfonate *F > Phenotypic information *F > Causes an increase in recombination between flanking markers *F > of 6.5-10.5 times, while recombination in other adjacent *F > regions is not changed. *F > *F > References *F > Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 *F > [FBrf0034851] *F > Dorer and Christensen, 1989, Genetics 122: 397--401 *F > [FBrf0049901] *F > Dorer and Christensen, 1990, Genetics 125: 795--801 *F > [FBrf0051978] *F > *F > Data from ref. FBrf0051978 *F > Synonym(s) Tpl[17] *F > Molecular biology data *F > Insertion of an unidentified mobile element. *F > Mutagen insertion *F > Phenotypic information *F > Allele class: hypomorph *F > Site specific increase in recombination frequency and premeiotic *F > recombination in males. *F *F ~Delete this allele for two reasons: (1) because it is an allele of Su(Tpl), *F ~listed in that entry, and (2) because it is NOT a revertant - that was an *F ~error in Lindsley and Zimm, and the renaming of it as Tpl[rv5] was *F ~inappropriate and incorrect. *F >Allele Tpl[rv5] *F > Synonym(s) tpl[38] *F > FlyBase ID number FBal0017002 *F > Mutagen ethyl methanesulfonate *F > Phenotypic information *F > Causes an increase in recombination between flanking markers *F > of 6.5-10.5 times, while recombination in other adjacent *F > regions is not changed. *F > *F > References *F > Roehrdanz andLucchesi, 1980, Genetics 95(2): 355--366 *F > [FBrf0034851] *F > Dorer and Christensen, 1989, Genetics 122: 397--401 *F > [FBrf0049901] *F > Dorer and Christensen, 1990, Genetics 125: 795--801 *F > [FBrf0051978] *F > *F > Data from ref. FBrf0051978 *F > Synonym(s) Tpl[38] *F > Molecular biology data *F > Insertion of an unidentified mobile element. *F > Mutagen insertion *F > Phenotypic information *F > Allele class: hypomorph *F > Site specific increase in recombination frequency and premeiotic *F > recombination in males. *F *F References *F Lindsley et al., 1972, Genetics 71: 157--184 [FBrf0023910] *F Denell, 1974, D. I. S. 51: 124 [FBrf0026052] *F Denell, 1976, Genetics 84: 193--210 [FBrf0028747] *F \* Kaufman, 1978, Genetics 90(3): 579--596 [Fbrf0031649] *F Keppy and Denell, 1979, Genetics 91: 421--441 [FBrf0033180] *F Roehrdanz and Lucchesi, 1979, Genetics 91(4/2): s105 [FBrf0033226] *F Lucchesi and Roehrdanz, 1979, Genetics 91(4/2): s71 [FBrf0033295] *F Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 *F [FBrf0034851] *F Roehrdanz and Lucchesi, 1981, Dev. Genet. 2(2): 147--158 *F [FBrf0036224] *F Kennison and Russell, 1987, Genetics 116: 75--86 [FBrf0046404] *F Christensen and Lucchesi, 1988, D. I. S. 67: 15 [FBrf0047737] *F Dorer and Christensen, 1989, Genetics 122: 397--401 [FBrf0049901] *F Dorer and Christensen, 1990, Genetics 125: 795--801 [FBrf0051978] *F Dorer et al., 1990, Nucleic Acids Res. 18: 5489--5494 *F [FBrf0052735] *F Dorer et al., 1993, Genetics 134(1): 243--249 [FBrf0058587] *F Christensen et al., 1993, Int. Congr. Genet. 17 Abstr.: 179 *F [FBrf0058726] *F Christensen and Dorer, 1989, A. Conf. Dros. Res. 30: 15 *F [FBrf0066351] *F Lindsley and Zimm, 1992 [FBrf0066905] *F Barry and Christensen, 1994, A. Conf. Dros. Res. 35: 319 *F [FBrf0068319] *F Christensen et al., 1995, A. Conf. Dros. Res. 36 Suppl.: 286A *F [Fbrf0078212] *F \* Dorer et al., 1995, Genetics 141(3): 1037-1042 *F *F *F *F *F *F --========================_64989128==_ *F Content-Type: text/plain; charset='us-ascii' *F *F \***************************************************************** *F \* Alan C. Christensen, Ph.D. '...there are those rare \* *F \* School of Biological Sciences characters who study the \* *F \* University of Nebraska unknown product of an \* *F \* Lincoln, NE 68588-0118 unknown gene. These we \* *F \* Phone 402-472-0681 call lunatics.' \* *F \* FAX 402-472-2083 -Sidney Brenner \* *F \***************************************************************** *F *F *F *F --========================_64989128==_-- *F *F *F From achristensen@crcvms.unl.edu Fri Jan 19 21:32:22 1996 *F Date: Fri, 19 Jan 1996 15:08:10 -0600 *F From: achristensen@crcvms.unl.edu (Alan C. Christensen, Ph.D.) *F Subject: Su(Tpl): revised FB entry *F To: flybase-updates@morgan.harvard.edu *F Mime-Version: 1.0 *F Content-Type: multipart/mixed; boundary='========================_64989128==_' *F Content-Transfer-Encoding: 7BIT *F Content-Length: 4794 *F Status: RO *F X-Lines: 144 *F *F *F *F --========================_64989128==_ *F Content-Type: text/plain; charset='us-ascii' *F *F Dear Colleagues, *F *F I have attached a revised version of the FB entry for Su(Tpl). This *F accompanies my previous update of Tpl. The symbols I have used for *F comments, additions or deletions are as described. If you have any *F questions or if my mailer does not attach the file properly to this message *F please let me know. *F *F Sincerely, *F Alan Christensen *F *F *F *F --========================_64989128==_ *F Content-Type: text/plain; name='Su(Tpl)'; charset='iso-8859-1' *F Content-Transfer-Encoding: quoted-printable *F Content-Disposition: attachment; filename='Su(Tpl)' *F *F ~Revised FB entry for Su(Tpl). *F ~Please note: *F ~Lines beginning with a \* indicate additions or changes to the current entry *F ~Lines beginning with a ~ indicate comments from me that explain why somethi= *F ng *F ~should be changed or deleted *F ~Lines beginning with a > indicate lines in the current entry that should *F ~be deleted *F *F Gene symbol Su(Tpl) *F =46lyBase ID number FBgn0014037 *F Date 13 Oct 95 *F *F \*Genetic map position 3-46.5 (Dorer et al., 1995); *F \* between st and ri. *F *F Cytological map position *F \* Located in 76B-D because included in Df(3L)kto[2] (Dorer et al., 1995) *F *F ~A better reference for this information would be Dorer et al, 1995 *F ~which I do not know the Fbrf# of (see below) *F Data from ref. FBrf0058587 *F Phenotypic information *F Mutations at Su(Tpl) block the triplo-lethal effect of Tpl. *F Several alleles previously thought to be hypomorphic mutations *F of Tpl are dominant mutations of the tightly linked Su(Tpl). *F \* Su(Tpl) mutations are also recessive lethal. *F *F Allele Su(Tpl)[10] *F Synonym(s) Tpl[10] *F Tpl[rv3] *F FlyBase ID number FBal0034034 *F \* Mutagen ethyl methanesulfonate *F \* *F \* References *F \* Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 *F \* [FBrf0034851] *F \* Dorer and Christensen, 1989, Genetics 122: 397--401 *F \* [FBrf0049901] *F \* Dorer and Christensen, 1990, Genetics 125: 795--801 *F \* [FBrf0051978] *F \* Dorer et al., 1995, Genetics 141(3): 1037-1042 *F \* [FBrf???????] *F *F \* Data from ref. FBrf0051978 *F \* Molecular biology data *F \* Possible insertion of an unidentified mobile element. *F \* Phenotypic information *F \* Site-specific increase in recombination frequency and premeiotic *F \* recombination in males. *F *F \*Allele Su(Tpl)[17] *F \* Synonym(s) Tpl[17] *F \* Tpl[rv4] *F \* FlyBase ID number FBal??????? *F \* Mutagen ethyl methanesulfonate *F \* *F \* References *F \* Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 *F \* [FBrf0034851] *F \* Dorer and Christensen, 1989, Genetics 122: 397--401 *F \* [FBrf0049901] *F \* Dorer and Christensen, 1990, Genetics 125: 795--801 *F \* [FBrf0051978] *F \* Dorer et al., 1995, Genetics 141(3): 1037-1042 *F \* [FBrf???????] *F *F \* Data from ref. FBrf0051978 *F \* Molecular biology data *F \* Possible insertion of an unidentified mobile element. *F \* Phenotypic information *F \* Site-specific increase in recombination frequency and premeiotic *F \* recombination in males. *F *F \*Allele Su(Tpl)[38] *F \* Synonym(s) Tpl[38] *F \* Tpl[rv5] *F \* FlyBase ID number FBal??????? *F \* Mutagen ethyl methanesulfonate *F \* *F \* References *F \* Roehrdanz and Lucchesi, 1980, Genetics 95(2): 355--366 *F \* [FBrf0034851] *F \* Dorer and Christensen, 1989, Genetics 122: 397--401 *F \* [FBrf0049901] *F \* Dorer and Christensen, 1990, Genetics 125: 795--801 *F \* [FBrf0051978] *F ~I don't know the Fbrf# of this, or if it has one yet *F \* Dorer et al., 1995, Genetics 141(3): 1037-1042 *F \* [FBrf???????] *F *F \* Data from ref. FBrf0051978 *F \* Molecular biology data *F \* Possible insertion of an unidentified mobile element. *F \* Phenotypic information *F \* Site-specific increase in recombination frequency and premeiotic *F \* recombination in males. *F *F \*Allele Su(Tpl)[J34] *F \* Synonym(s) Tpl[J34] *F \* FlyBase ID number FBal??????? *F *F \* Data from ref. Fbrf0058587 and Dorer at al., 1995 *F Phenotypic information *F \* Phenotypic class: suppressor | dominant, lethal | recessive *F Suppresses the triplo-lethal effect of Tpl. *F \* Data from ref. FBrf0051978 *F \* Mutagen PM hybrid dysgenesis *F \* Associated aberration(s) P{}Tpl[J34] *F *F References *F Dorer et al., 1993, Genetics 134(1): 243--249 [Fbrf0058587] *F ~I don't know the Fbrf# of this, or if it has one yet *F \* Dorer et al., 1995, Genetics 141(3): 1037-1042[FBrf???????] *F --========================_64989128==_-- # *U FBrf0084556 *a Thaker *b H. *t 1995.11.7 *T personal communication to FlyBase *u *F Archived # *U FBrf0084558 *a Thaker *b H. *t 1995.12.4 *T personal communication to FlyBase *u *F Date: Mon, 4 Dec 95 10:03:30 -0800 *F From: thaker@cmgm.stanford.edu *F To: Eleanor Whitfield (Genetics) *F Subject: Re: correction *F Cc: thaker@cmgm.stanford.edu *F Content-Type: TEXT/plain; charset=US-ASCII *F Content-Length: 227 *F Hello Eleanor: *F I have word from Doug Kankel's lab about the surviving lethals from my *F study. *F They are: *F l(1)TK110, 111, 132, 136, 225, 236, 237, 302, 308, 309, 313, 410 and 411. *F The rest have been lost. *F Hope this helps, *F Harsh Thaker # *U FBrf0084560 *a Wright *b T.R.F. *t 1995.12.1 *T personal communication to FlyBase *u *F Date: Fri, 1 Dec 1995 11:33:42 -0500 *F From: 'Theodore R. Wright' *F The cytology on Dp(2;2)M(2)m+ was never done in my lab and *F I don't know that anyone else has ever done it. Genetically it *F appears to be fairly large since it rescues dominant Fs alleles of *F dl (Fs7606 = dl7) at 36C and a dominant Fs allele of l(2)37Cc which *F is also a recessive lethal allele at the locus = l(2)37Cc13 at 37C. *F We picked the duplication up accidently when it rescued the Minute *F phenotype of one of our deficiencies that deletes the Minute in the *F 36F region. Subsequently we used it in numerous stocks that delete *F this Minute to enhance the viability of the stocks. It also rescues *F Df(2L)M36F-S6 and Df(2L)M36F-S5. There is no information on the size *F of the duplication nor its location on the CyO chromosome. I hope *F someone feels like doing the cytology and lets me know the details. # *U FBrf0084561 *a Matthews *b K. *t 1995.11.17 *T personal communication to FlyBase *u *F From: Kathy Matthews, Indiana University *F To: FlyBase *F Subject: Variants of TM6B, TM3 and CyO *F Date: 17 Nov 95 *F *F Background: These new balancer variants now in the Bloomington Stock Center need to be added to FlyBase. *F *F Variants of TM6B *F Genotype: P{35UZ} *F Genotype short name: TM6B-UZ *F Pattern info: strong lacZ expression in epidermis, nerve cord PS 6-12, midgut PS 7, stages 11-17 (pc to Bloomington Stock Center *F from David Bilder, possibly published by K. Irvine) *F *F Variants of TM3 *F Genotype: P{HZ2.7} *F Genotype short name: TM3-DZ *F Pattern info: strong lacZ expression in maxillary segment stages 11-17 (pc to Bloomington Stock Center from David Bilder, likely published *F by W. McGinnis) *F *F Variants of CyO *F Genotype: P{lArB}Cg25c[A109] *F Genotype short name: CyO-CZ *F Pattern info: lacZ expression essentially ubiquitous *F Genotype: P{en.96-HZ73(ry)}wg[en11] *F Genotype short name: CyO-WZ *F Pattern info: strong lacZ expression in epidermal stripes, PS 8 midgut, proventriculus, weak nerve cord stages 10-17 (pc to Bloomington *F Stock Center from David Bilder, but may be published elsewhere.) *F *F These 'Genotype short names' are those used by the donor of the stock. # *U FBrf0084562 *a Artero *b R.D. *t 1995.11.17 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Ruben D. Artero, Valencia University (Universitat de Valencia) *F To: Bloomington Drosophila Stock Center *F Subject: no reversions for l(2)01038[01038] and l(2)07129[07129] *F Date: 17 November 1995 *F *F Information communicated: *F *F "I am sending this message to report our results with two P element insertions from the Berkeley Drosophila genome project after *F transposase movilize them. *F *F 1. P976 (l(2)01038). After movilization we did not recover any viable revertant and concluded that there must be another lethal in this *F chromosome (the strain behaves as lethal). The same result was obtained, independently, by Gerrit Begemann (Marek Mlodzik's *F group; personal communication). We are about to 'clean' this insertion. *F *F 2. P2336 (l(2)07129). We made this movilization at the same time as the one for P976 but we did not recover any revertant at all. *F Maybe there is some problem with this insertion? (we could not plasmid rescue this strain either) Both strains are kept here since *F three years ago,more or less. We hope this could be of interest for the Drosophila genome project." *F # *U FBrf0084563 *a Martin *b P. *t 1995.12.5 *T personal communication to FlyBase *u *F Archived # *U FBrf0084564 *a Skaer *b R.J. *t 1995.12.11 *T personal communication to FlyBase *u *F Archived # *U FBrf0084566 *a Andres *b A. *t 1996.1.5 *T personal communication to FlyBase *u *F From aan838@lulu.acns.nwu.edu Fri Jan 5 01:58:27 1996 *F Date: Thu, 04 Jan 1996 19:53:20 -0600 *F From: aan838@lulu.acns.nwu.edu (Andrew Andres) *F Subject: 63F map update *F To: flybase-updates@morgan.harvard.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-Transfer-Encoding: 7BIT *F Content-Length: 1254 *F Please update the following for the 63F region. *F Eip63Fb should be called Eip63F2. Its synonym (E63-2) is correct, but it *F does not encode a putative Ca2+-binding protein. It produces a single *F transcript of 1.25 kb, but it's function at present is unknown, ie there *F are no significant matches in the database. The accession number is *F correct, but the correct reference for its cloning and sequencing is: A. J. *F Andres and C. S. Thummel, Development 121:2667-2679 (1995). *F Eip63Fa should be called Eip63F1. Its synonym is E63-1. and it encodes a *F 193 aa Ca2+-binding protein with four putative Ca2+-binding domains. It is *F most similar to calmodulins with approx. 35% similarity overall. It *F produces three transcripts of 2.8, 1.7, and 1.1 kb. All three are induced *F by ecdysone in the salivary gland, all three share the same 5' ends, and *F all three encode the same open reading frame. The accession number for *F Eip63F1 is U25882, and the reference for its cloning and sequencing is the *F same as above: A. J. Andres and C. S. Thummel, Development 121: 2667-2679 *F (1995). *F Thanks for updatining the flybase info on these two genes. *F Andrew Andres *F Dept of Molec Pharm & Biol Chem *F Northwestern University *F 303 E. Chicago Ave *F Chicago, IL 60611 *F 312-503-0082 *F From rd120@gen.cam.ac.uk Fri Jan 5 11:43:58 1996 *F Date: Fri, 05 Jan 1996 11:37:57 +0000 (GMT) *F From: Rachel Drysdale (Genetics) *F Subject: Re: 63F map update *F To: aan838@lulu.acns.nwu.edu *F Cc: flybase-updates@morgan.harvard.edu *F Content-Transfer-Encoding: 7BIT *F Content-Length: 879 *F Dear Andrew, *F Thank you for your updates about Eip63Fa and Eip63Fb. *F Unfortunately there is a small (though not insurmountable!) problem with the *F symbols you suggest. *F Eip63F1 suggests that the gene maps to band 63F1, and Eip63F2 suggests that *F the gene maps to band 63F2. If this is not the case, and you do not know *F the cytological locations of these genes to that degree of accuracy, may I *F suggest the symbols Eip63F-1 and Eip63F-2, which avoids that problem. *F best wishes, *F Rachel *F From aan838@lulu.acns.nwu.edu Fri Jan 5 15:25:04 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 5 Jan 1996 09:20:05 -0600 *F To: Rachel Drysdale (Genetics) *F From: aan838@lulu.acns.nwu.edu (Andrew Andres) *F Subject: Re: 63F map update *F Content-Length: 1018 *F Dear Rachel, *F I agree with your analysis and think that Eip63F-1 and Eip63F-2 are much *F better names. *F Thanks # *U FBrf0084567 *a Adam *b G. *t 1996.1.17 *T personal communication to FlyBase *u *F Transposon information for two lines submitted to the Bloomington Stock *F Center, stocks and information from Geza Adam, Friedrich Miescher Institute, *F Basel, Switzerland. *F From: adam@fmi.ch *F Date: Wed, 17 Jan 96 13:17:18 +0100 *F This is the requested information about the UAS-M2C48C (MAP2c) and *F UAS-TAU59A (tau) lines. The MAP2c sequences were from rat (Doll, T. *F et al. 1990, Nucl. Acid Res. 18, 361) and the tau (htau23) sequences *F were from human (Goedert, M. et al. 1989, Neuron 3, 519-526) source. *F Both sequences were cut off from pBluescript vector with NotI and *F Bsp 120I restriction endonucleases and built in the NotI site of pUAST *F vector. MAP2c and tau constructs were refered to as pUAST-MAP2c and *F pUAST-htau23, respectively. *F Both constructs were injected into yw/yw strain (w allele was w[1118]). *F The insertion site of the transgene were not determined, in both cases *F they are on the second chromosome. Both transgenic lines are viable as *F homozygotes and after induction no mutant phenotype was observable. # *U FBrf0084568 *a Doberstein *b S. *t 1996.1.23 *T personal communication to FlyBase *u *F From steve@well.com Mon Jan 22 23:48:35 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-Mailer: Eudora Pro 2.1.3 *F Date: Mon, 22 Jan 1996 15:39:38 -0800 *F To: Rachel Drysdale (Genetics) *F From: steve@well.com (Steve Doberstein) *F Subject: Re: FlyBase Help Mail - nomenclature *F Content-Length: 1194 *F Dear Rachel: *F I wrote you a few months ago about using the names blown fuse *F and/or sour grapes as mutant names. We decided to go with blown fuse, blow *F for short, to rename l(2)43Eb as isolated by Heitzler et al. The mutant *F phenotype is that myoblasts fail during fusion to form myotubes. We've *F cloned this gene, which appears to be totally novel. *F Steve *F From steve@well.com Mon Jan 22 23:48:35 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-Mailer: Eudora Pro 2.1.3 *F Date: Mon, 22 Jan 1996 15:39:38 -0800 *F To: Rachel Drysdale (Genetics) *F From: steve@well.com (Steve Doberstein) *F Subject: Re: FlyBase Help Mail - nomenclature *F Content-Length: 1194 *F >For everything to be perfect, it would be nice if you told me the allele *F >that the mutant phenotype goes with. Is it the original l(2)43Eb[1] or *F >have you made another one? *F Both l(2)43Eb[1] and one other allele we've generated here in the lab. *F We're calling them like so: l(2)43Eb[1] = blow[1] *F BB034 (our original name) = blow[2] *F thanks *F Steve # *U FBrf0084569 *a Schulze *b K.L. *t 1996.1.24 *T personal communication to FlyBase *u *F Date: 1-24-96 *F Name: Karen L. Schulze *F Address: *F Division of Neuroscience, Baylor College of Medicine, *F One Baylor Plz., Houston TX 77030 USA *F Telephone No.: (713) 798-5273 *F FAX No.: (713) 798-8515 *F E-mail address: kschulze@imgen.bcm.tmc.edu *F Corrections to FlyBase ID No. FBgn0013343 *F Gene Symbol syx1A *F Data from references FBrf0078115 and NEW *F Phenotypic info: *F Broadie et al., 1995, Neuron 15:663-673 (NEW reference) *F Syx1A is absolutely required for spontaneous vesicle *F fusions, for these events are absent in syx1A null mutants. *F However, mature, docked synaptic vesicles are observed by *F EM at release sites in the presynaptic terminals of syx1A *F mutant synapses. Therefore, syntaxin functions downstream *F of vesicle docking and participates in fusion. *F Data from references FBrf0080377 and FBrf0079352 *F Phenotypic info: *F The syx1A gene product plays a role in general secretion and *F is required for exocytosis from a variety of secretory *F tissues. Syx1A is absolutely required for evoked neuro- *F transmission as lack of Syx1A entirely abolishes evoked *F neurotransmitter release. *F Data from references FBrf0080377 and NEW *F synonym(s): *F syx1A *F syx *F \*** *F Karen Schulze # *U FBrf0084570 *a Gould *b A. *t 1996.1.24 *T personal communication to FlyBase *u *F Date: Wed, 24 Jan 1996 19:52:54 +0000 *F From: a-gould@nimsn41.nimr.mrc.ac.uk (Alex Gould) *F Here's what I know about i71 (or just 71), most of which was only *F described in my thesis and never published properly. *F I isolated 71 which is homozygous viable, along with three other *F insertions, in a PlacZ screen for segmentally-modulated enhancer traps. *F Expression occurs in PS3-13 but is more intense in PS4-5 (I have X-gal *F pictures). This pattern is reminiscent of the Antp P2 transcript *F distribution. The transposon used was from the plasmid pLacA92 as first *F described by O'Kane and Gehring. This was mobilised by jumpstart from an *F X-insertion line called c49. We did some chromosome in situs and thought *F the 71 insertion was probably at 40F (as did Michael Ashburner). However, *F given that the expression pattern was so similar to teashirt we sent the *F stock to Steve Kerridge. He did some Southerns and I think he failed to *F find the transposon within his teashirt genomic walk. I am not sure whether *F he redid the lacZ chromosome in situs. # *U FBrf0084571 *a Kerridge *b S. *t 1996.1.25 *T personal communication to FlyBase *u *F Date: Thu, 25 Jan 96 09:52:09 +0100 *F From: 'Stephen KERRIDGE' *F Concerning Alex Gould's insert P{A92}i71: *F We've cloned around 150kb around tsh and the only insert we failed to *F localize on this walk (we have about 10 others) was Alex's. However I *F cannot guarentee we were 100% critical about these southerns, especially *F as this P element has no plasmid rescue element. The beta-gal pattern of *F P{A92}i71 is certainly similar, and in some cases identical, to all the *F other P{tsh} insertions. Genetically it behaves like the others, in that *F over a deletion such as Df(2L)TW161, the proximal leg segments in adults *F are affected. Finally we did in situs to localize Alex's insertion; it is *F definately in 40A and not 40F. In other words I'm sure its nearby (to tsh) *F somewhere! *F Second message: *F P{A92}71 has been looked over a tsh null. All P elements behave the same for *F example over a null like tsh, but the phenotype is weaker than over Df(2L)TW161. *F This (I believe) can be explained by the fact that tsh8 has regulatory elements *F that can act in trans on the insertion chromosome to make tsh 'more' active. *F Df161 does not have these regulatory elements. Molecularly we don't know where P{A92} inserts with respect to tsh, in P{A92}71. # *U FBrf0084572 *a Roote *b J. *t 1996.1.11 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Thu Jan 11 18:46:44 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 11 Jan 1996 18:41:05 +0100 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: changes to flybase *F Content-Length: 2943 *F Additions: *F \*a Df(2L)el94 *F \*o spontaneous *F \*G l(2)35Aa << bk1 << el << pu << bk2 << noc *F \*w Roote *F \*a Df(2L)H60-3 *F \*w Tower *F \*o male recombination *F \*G l(2)35Fb, l(2)35Fc << bk1 << l(2)35Ff << cact << bk2 << chif, l(2)35Fe *F nBR100 \*w Lee \*o tritium \*G l(2)34Fa << bk1 << wb << Adhr << bk2 << l(2)35Bb *F nBR102 \*w Lee \*o tritium \*G elA << bk1 << noc << esg << bk2 << l(2)35Da *F nBR103 \*w Lee \*o tritium \*G l(2)34Fa << bk1 << wb << l(2)35Dc << bk2 << *F l(2)35Dd *F nBR105 \*w Lee \*o tritium \*G noc << bk1 << osp << stc << bk2 << l(2)35Cc, rd *F nBR106 \*w Lee \*o tritium \*G elA << bk1 << noc << ck << bk2 << l(2)35Cf *F (comment: breaks within noc) *F nBR107 \*w Lee \*o tritium \*G noc << bk1 << osp << ck << bk2 << l(2)35Cf *F nBR118 \*w Lee \*o tritium \*G elA << bk1 << noc << esg << bk2 << l(2)35Da *F nBR119 \*w Lee \*o tritium \*G elA << bk1 << noc << Adhr << bk2 l(2)35Bb *F (comment: breaks within noc) *F nBR120 \*w Lee \*o tritium \*G l(2)34Fa << bk1 << wb << l(2)35Ea << bk2 << *F ms(2)35Eb *F nBR121 \*w Lee \*o tritium \*G noc << bk1 << osp << Adhr << bk2 << l(2)35Bb *F nBR122 \*w Lee \*o tritium \*G noc << bk1 << osp << stc << bk2 << l(2)35Cc, rd *F nBR125 \*w Lee \*o tritium \*G l(2)34Fa << bk1 << wb << esg << bk2 << l(2)35Da *F nBR126 \*w Lee \*o tritium \*G elA << bk1 << noc << BicC << bk2 << l(2)35Fa *F (comment: breaks within noc) *F nBR127 \*w Lee \*o tritium \*G noc << bk1 << osp << Adhr << bk2 << l(2)35Bb *F nBR128 \*w Lee \*o tritium \*G noc << bk1 << osp << stc << l(2)35Cc, rd *F nBR129 \*w Lee \*o tritium \*G elA << bk1 << noc << l(2)35Cd << bk2 << esg *F nBR130 \*w Lee \*o tritium \*G l(2)34Da << bk1 << l(2)34Db << sna << bk2 << lac *F nBR131 \*w Lee \*o tritium \*G rk << bk1 << l(2)34Fa << stc << bk2 << l(2)35Cc, rd *F Changes: *F chif64 \*G fzy << bk1 << cact << dac << bk2 (breaks within cact) *F T(Y;2)el[4D]A80[P] \*G l(2)35Aa << bk1 << elB << pu << bk2 << elA *F (comment: breaks within elB) *F T(Y;2)el[5D]A80[P] \*G l(2)35Aa << bk1 << elB << pu << bk2 << elA *F (comment: breaks within elB) *F T(Y;2)el[4D]R15[P] \*G l(2)35Aa << bk1 << elB << l(2)35Bg, Su(H) << bk2 << *F ck (comment: breaks within elB) *F In(2LR)el[6L]A379[R] \*G l(2)35Aa << bk1 << elB << Adhr << bk2 << osp *F (comment: breaks within elB) *F T(2;3)dpp[s19D]el24[P] \*G l(2)35Aa << bk1 << elB << elA << bk2 << noc *F (comment: breaks within elB) *F fn12 \*G bk1 << rk << ck << bk2 (i.e. not mapped precisely) *F fn15 \*G bk1 << el << ck << bk2 ditto *F GT5 \*G bk1 << b << noc << bk2 ditto *F T(Y;2)GT2[D]A80[P] \*G l(2)35Aa << bk1 << elB << pu << bk2 << elA *F noc11 \*G j << bk1 << rk << l(2)35Cd << bk2 << esg (comment: breaks within rk) *F nNXF1 \*G elA << bk1 << noc << stc << bk2 << l(2)35Cc,rd (comment: *F breaks within noc) *F nNXF2 \*G elA << bk1 << noc << stc << bk2 << l(2)35Cc,rd (comment: *F breaks within noc) # *U FBrf0084573 *a Zimm *b G.G. *c D.L. *d Lindsley *t 1996.1.25 *T personal communication to FlyBase *u *F From zimm@jeeves.ucsd.edu Fri Jan 26 05:06:57 1996 *F Date: Thu, 25 Jan 1996 21:00:24 -0800 (PST) *F From: Georgianna Zimm *F To: flybase-updates@morgan.harvard.edu *F Content-Transfer-Encoding: 7BIT *F Content-Length: 4047 *F Zimm, G.G. and D.L Lindsley. Department of Biology, University of *F California, San Diego, La Jolla, CA 92093-0322. *F Upturned revisited with description of a new allele. *F Among the progeny of phenotypically wild-type males treated with ethyl *F methanesulfonate, one fly with curly wings was recovered; the phenotype was *F subsequently inherited as a dominant mutation that segregated with *F chromosome 2; crossing over with al b c px sp was normal, and no *F straight-winged recombinants with c (2-75.5) were recovered among 161 *F testcross progeny. This result places the new mutation in the vicinity of *F Upturned (U). The allele U[1] is associated with a pericentric inversion *F with breakpoints at 40F and 53A according to Bridges and Li (Morgan, *F Bridges, and Schultz, 1935). The genetic map position originally given for *F U was 70+/- based on the mapping of a putative second allele, U[H20], *F isolated by Tanaka (1937); more recently Ashburner has revised this *F position to 2-[76], based on the 53A breakpoint of In(2LR)U[1], (FlyBase, *F 1994). In(2LR)U[1] is homozygous lethal, and U[H20], now lost, was *F homozygous viable. The new mutation is also homozygous lethal, but it *F survives in heterozygous combination with U[1]. If the new mutation is an *F allele of U, then at least one of the above two is not a lethal allele. To *F clarify this ambiguity, we generated an X-ray-induced revertant of U[1] and *F found that it is lethal in combination with the new mutant; thus we *F conclude that the new mutation is a lethal allele of U, whereas U[1] is a *F viable allele with a lethal mutation elsewhere on the chromosome; we *F designate the new allele U[2]. U[1]/U[2], U[1]/Cy , and U[2]/Cy flies *F show an additive effect of the curly-wing phenotype. *F We have been unsuccessful in finding a deficiency that uncovers the *F lethality of U[2]. The fact that neither four deficiencies for c at 52D1-7 *F (Saxton, pers. comm.; FlyBase, 1994) extend far enough to the right to *F include 53A, coupled with our inability to establish stocks of nearly all *F Xray-induced revertants of either U[1] or U[2], implies the existence of *F haplo-insufficiency that is coincident with or closely linked to Upturned. *F No duplications are available to cover the 53A1 breakpoint of In(2LR)U[1]. *F The viability of U[1] over Df(2L)C', a deficiency for the 2L *F heterochromatin (Hilliker and Holm, 1975), indicates that the lethal *F mutation in In(2LR)U[1] is not associated with the breakpoint at 40F. *F We were unable to cover the lethality of U[1] in male homozygotes with *F Dp(2;Y)G, a duplication that includes the 40F salivary region. *F In our hands U[1] (but not U[2]) exhibits reduced penetrance, with its *F expression depending on genetic background and in general being less *F reliable in males than in females. In the original description (Bridges, *F 1935), U[1] was said to cause, in addition to upwardly curled wings, *F crossed posterior scutellar bristles, darker than normal body color with *F dark waxy wings, and eyes mottled with light flecks. Of these phenotypes *F only upturned wings, crossed posterior scutellars, and darker than normal *F body color are currently observed in U[1] and U[2] heterozygotes and *F U[1]/U[2] heteroalleles. Since U[1] is associated with a pericentric *F inversion with one break in proximal 2L heterochromatin, we speculated that *F perhaps U[1] was originally recovered in combination with a lt-bearing *F homologue and that the eye mottling was variegation associated with removal *F of the lt locus from its normal heterochromatic to a distal euchromatic *F environment in In(2LR)U[1]; U[1]/lt flies exhibit variegated eyes in *F addition to upturned wings, crossed postscutellar bristles, and darker body *F color. *F References: *F Bridges, 1935, DIS 3: 5-19; *F Bridges, 1937, DIS 7: 5-17; *F Davis and MacIntyre, 1988, Genetics 120: 755-66; *F FlyBase, 1994, Nucleic Acids Res. 22: 3456-58; *F Hilliker and Holm, 1975, Genetics 81: 905-21; *F Morgan et al., 1935-36 , Year Book - Carnegie Inst. Washington 35:289-97; *F Saxton, pers. comm.; *F Tanaka, 1937, DIS 8: 11. # *U FBrf0084574 *a Roote *b J. *t 1996.1.30 *T personal communication to FlyBase *u *F Date: Tue, 30 Jan 1996 16:27:52 +0100 *F To: Rachel Drysdale (Genetics) *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: synonyms *F I think Carlos (Flores) was a little premature (see Flores and Engels, *F 1995, A. Conf. Dros. Res. 36 Suppl.: 204A [FBrf0078419]) *F spel1 is adjacent to 35Aa, but not the same. It has no phenotype when *F deleted. *F I wrote the following to him: *F 'Do I understand correctly the following: *F 1) spel1 is proximal of 35Aa *F 2) both 35Aa and spel1 are in the overlap of GW7 and b84h1 *F 3) the fragment -245 -- -250 rescues 35Aa/35Aa and GW7/b84h1 but does not *F include spel1 - therefore spel1[-] has no phenotype?' *F He replied YES! *F So the story is that he rescued the lethality of the overlapping deletions *F TE35B-7 and b84h1 with a fragment that included 35Aa but not spel1. The *F overlap includes 35Aa and spel1. # *U FBrf0084575 *a Kerridge *b S. *t 1996.1.31 *T personal communication to FlyBase *u *F Date: Wed, 31 Jan 96 14:01:02 +0100 *F From: 'Stephen KERRIDGE' *F P{wA[R]}4-3 localizes to the 3' end of the tsh gene. # *U FBrf0086241 *a Feyereisen *b R. *c B.C. *d Dunkov *t 1995.11.2 *T personal communication to FlyBase *u *F Archived. # *U FBrf0086242 *a Coulter *b D. *t 1995.9.27 *T personal communication to FlyBase *u *F From Coultede@wpogate.slu.edu Wed Sep 27 20:28:17 1995 *F X-Mailer: Novell GroupWise 4.1 *F Date: Wed, 27 Sep 1995 14:23:44 -0600 *F From: Douglas E. Coulter *F To: m.ashburner@gen.cam.ac.uk *F Subject: Flybase: odd related genes *F Content-Length: 2995 *F *F Michael *F Forgive my delay in responing to your request for odd related gene info. *F The two genes you have listed as odd-skipped related (org) 1 and 2 we have *F renamed as #sob# and bwl#, respectively. Each name is an acronym: sob = *F sister of odd and bwl; bwl = brother of odd with entrails limited. Both *F genes encode proteins with five Cys-His zinc fingers, with the four *F N-terminal fingers of each matching (with 86-88% amino acid identity) the *F four fingers of odd. (Over all five fingers, sob and bwl show 95% amino *F acid identity.) *F *F By the way, Flybase and Lindsley and Zimm include a listing for #Df(2L)odd# *F that I suspect is a phantom (if not, we may have been wasting our time with *F mutageneses). The reference given is the 1984 Roux#s Archives 2nd *F chromosome paper of Nusslein-Volhard et al, but no such chromosome is *F listed there. The confusion might have stemmed from the use of a synthetic *F deficiency for the region (23E-24B) generated by crossing T(2;3)odd[5.1] *F and T(1;2)odd[1.10] that was cited in a later Nusslein-Volhard paper. The *F gene that is distal to sob (org1) that Steve Cohen has mentioned to you is *F undoubtedly Alp, rather than bowel (org2). bowel maps between distal *F breakpoints of Df(2L)sc[19-8] and Df(2L)ed-dp[h1], which are app. 30 kb *F apart on our molecular map, and T(Y;2)L126 breaks within the second bwl *F intron. This definitely puts bwl proximal to odd and sob, nominally in *F 24C2-5. *F *F Doug Coulter # *U FBrf0086243 *a Gelbart *b W.M. *t 1995.10.13 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Fri Oct 13 13:56:47 1995 *F Date: Fri, 13 Oct 95 08:53:27 EDT *F From: gelbart@morgan.harvard.edu (William Gelbart) *F To: eleanor@gen.cam.ac.uk, mashburner@morgan.harvard.edu *F Subject: Re: HELP - held out alleles *F Cc: gelbart@morgan.harvard.edu *F Content-Length: 1423 *F *F Nellie & Michael, *F *F In the 1982 Spencer et al paper in Cell (our first paper on *F dpp), we describe an allele that we called dpp[ho2] and which was *F transmogrified to dpp[d-ho2] when we revamped the nomenclature of *F the gene to describe the different major cis-regulatory domains. *F dpp[d-ho2] was generated as a heldout allele over a chromosome *F that was designated 'ast dpp[d-ho] ed dp cl'. We didn't have *F rearranged chromosomes with dpp[d-ho] to use as testers at that *F time. Several of the early papers on dpp indeed refer to dpp[d-ho2] *F as a distinct allele. However, when we finally cloned dpp and *F characterized dpp[d-ho] and dpp[d-ho2], we found that they were *F molecularly identical at the restriction map level, carrying the *F same deletion of ~112 to 114.8 on our molecular map. While we did *F not sequence over the deletion breakpoints to absolutely confirm *F this, we now consider them identical and refer to them both as *F dpp[d-ho]. In retrospect, there was probably a crossover between *F dpp[d-ho] and ed to produce an unmarked chromosome bearing the *F dpp[d-ho] allele and we misguidedly saved it as a new allele. (We *F learned far after the fact that this 'ast dpp[d-ho] ed dp cl' *F chromosome is actually ast[+].) Thus, dpp[d-ho2] = dpp[d-ho]. *F *F I believe dpp[ho2c] comes from Table 1 on page 288 of *F Smolik-Utlaut, 1987. Because of the way the journal typeset the footnotes, *F the footnote [c] looks like it's part of the allele name dpp[ho2]. *F The footnote [c] simply is a reference to the original citation for *F this allele (Spencer et al., 1982). *F *F Have you guys also created a synonym dpp[4c], which should really *F be dpp[4] with the same [c] footnote? If not, why not? ;-) *F *F In other words, chuck dpp[ho2c]. *F There you have it. *F *F Bill # *U FBrf0086244 *a Perrimon *b N. *t 1995.8.30 *T personal communication to FlyBase *u *F From perrimon@rascal.med.harvard.edu Wed Aug 30 12:24:14 1995 *F Date: Wed, 30 Aug 95 12:24:03 BST *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: rd120@gen.cam.ac.uk *F From: perrimon@rascal.med.harvard.edu (Norbert Perrimon) *F Content-Length: 428 *F *F Dear Rachel, *F We recovered two independent insertions at the wg locus (28A) in the *F Kassis et al. 1992 study. Both of them have the wg embryonic lethal *F phenotype and expresses lacZ in the wg pattern. *F One is called 1en-11 and is inserted on a CyO chromosome. We write it CyO, *F wg1en11 *F The other one is 17en40 and is inserted on a wild type chromosome. *F Let me know if you need further information. *F *F Best wishes, *F *F Norbert Perrimon # *U FBrf0086245 *a Roote *b J. *t 1995.9.26 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Sep 25 18:44:04 1995 *F Mime-Version: 1.0 *F Date: Mon, 25 Sep 1995 18:39:48 +0100 *F To: Eleanor Whitfield (Genetics) *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: 43 lethals *F *F *F Eleanor, *F *F Attached is a list of 43 lethals - all the info that I have. *F *F J *F *F nec *F nece3 pke3, In(2R)nec3 pk3, 41; 43A1.2, necv pk bwD, EMS *F necx5 pkx5, In(2R)nec5 pk5, 41BC; 42F1.2-43A1, necv pk cn bw sp, EMS *F GJ63-6, T(2;3) nec14, 43A;80;87, b necv pk-splev cn, X-Rays *F GJ60-48, nec15, +, b nec cn bwv, X-Rays *F DE33, nec16, +, b nec cn, EMS *F GJ61-4, nec17, +, b nec cn, X-Rays *F GJ63-1, nec18, +, b nec cn, X-Rays *F 7, DE25, EMS, cn bw sp *F 8, DE33, EMS, cn bw sp *F 9, DE43, EMS, cn bw sp *F 10, DE49, EMS, cn bw sp *F 11, DE51, EMS, cn bw sp *F *F l(2)43Ba *F 1, Ew3, EMS, bw[D] *F 2, Ew8, EMS, bw[D] *F 3, NCX3, X, cn bw sp *F 4, DE21, EMS, cn bw sp *F 5, DE26, EMS, cn bw sp *F 6, DE29, EMS, cn bw sp *F 7, DE37, EMS, cn bw sp *F 8, DX2, X, cn bw sp *F *F pwn *F 6, DE18, EMS, cn bw sp *F 7, DE38, EMS, cn bw sp *F *F l(2)43Bb *F 1, Ew4, EMS, bw[D] *F 2, Ew24, EMS, bw[D] *F 4, DE32, EMS, cn bw sp *F 5, DE56, EMS, cn bw sp *F 6, PZ04614, P, cn *F 7, DX11 (Tp(2;3)41-43B1.3;80), X, cn bw sp *F *F l(2)43Bc *F 1, Ew2, EMS, *F 2, Ew10, EMS, *F 3, DX4 (In(2R)41;43B1.3), X, cn bw sp *F 4, DE22, EMS, cn bw sp *F 5, DE27, EMS, cn bw sp *F 6, DE28, EMS, cn bw sp *F 7, DE39, EMS, cn bw sp *F 8, DE44, EMS, cn bw sp *F 9, DE46, EMS, cn bw sp *F 10, DE47, EMS, cn bw sp *F 11, DE54, EMS, cn bw sp *F 12, DE59, EMS, cn bw sp *F 16, DE59, EMS, cn bw sp *F 17, DX8, X, cn bw sp *F 19, PZ05518, P, cn *F *F cos *F 14, DE23, EMS, cn bw sp *F 15, DE31, EMS, cn bw sp *F 16, DE52, EMS, cn bw sp *F *F l(2)43Bd *F 1, Ew1, EMS, bw[D] *F 2, Ew11, EMS, bw[D] *F 4, Ew9, EMS, bw[D] *F 5, DE19, EMS, cn bw sp *F 6, DE50, EMS, cn bw sp *F 7, k13522, P, + *F *F humilis *F 3, Ew11, EMS, bw[D] *F 8, DE24, EMS, cn bw sp *F *F so *F 5, 2AA, EMS, b cn *F 6, DE30, EMS, cn bw sp *F *F l(2)43Ca *F 1, Ew15, EMS, bw[D] *F 3, T(2;3)H36, X, *F *F l(2)43Cb *F 1, NCX6, X, cn bw sp *F *F l(2)43Cc *F 4, NCX7, X, cn bw sp *F *F l(2)43Da *F 1, Ew6, EMS, bw[D] *F *F l(2)43Db *F 1, Ew7, EMS, bw[D] *F *F l(2)43Ea *F 1, Ew23, EMS, bw[D] *F *F l(2)43Eb *F 1, Ew22, EMS, bw[D] *F *F l(2)43Ec (=scraps) *F 7, NCX1, X, cn bw sp *F 8, NCX2, X, cn bw sp *F *F l(2)43Ee *F 1, Ew18, EMS, bw[D] *F *F l(2)43Ef *F 1, Ew5, EMS, bw[D] *F *F cn *F EW31, Ew31, EMS, bw[D] *F EW32, Ew32, EMS, bw[D] # *U FBrf0086246 *a de Celis *b J.F. *t 1995.6.30 *T personal communication to FlyBase *u *F From jdc@mole.bio.cam.ac.uk Thu Jun 29 17:54:10 1995 *F Date: Thu, 29 Jun 1995 17:57:22 +0059 (BST) *F From: 'Jose de Celis (Genetics)' *F Subject: Re: dfr and vvl *F To: ag24 *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 189 *F Status: RO *F X-Lines: 7 *F *F Hi! *F The paper in which vvl and dfr are identified as being the same thing is now *F submitted to Development. In the mean time you can perfectly used de *F Celis personal communication! *F jose # *U FBrf0086247 *a Ashburner *b M. *t 1995.4.11 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Tue Apr 11 19:27:05 1995 *F From: Michael Ashburner (Genetics) *F Date: Tue, 11 Apr 95 19:29:18 BST *F To: ag24@gen.cam.ac.uk *F Subject: g u *F Cc: ma11@gen.cam.ac.uk *F Content-Length: 319 *F X-Lines: 15 *F Status: RO *F *F Aubs *F *F Please curate this as *F \*x FBrf... == M. Ashburner, Personal communication to FlyBase, 11 April 1995 *F *F \*a l(2)35Cf *F \+ *F \*A l(2)35Cf[1] *F \*i l(2)35Cf[481] *F \*x FBrf... == M. Ashburner, Personal communication to FlyBase, 11 April 1995 *F \*R Induced on bw *F \*w M. Goldsmith *F \*o P-element insertion \PM-hybrid dysgenesis *F \*O &pgr;2 # *U FBrf0086248 *a Roote *b J. *t 1994.1.4 *T personal communication to FlyBase *u *F From JR32@phx.cam.ac.uk Tue Jan 4 16:32:37 1994 *F Date: Tue, 04 Jan 94 16:28:52 GMT *F From: JR32@phx.cam.ac.uk *F To: ag24@phx.cam.ac.uk, ma11@phx.cam.ac.uk *F Content-Length: 690 *F Status: RO *F X-Lines: 29 *F *F Aubrey, *F *F 2 alterations to the TE list that MA sent you a few weeks ago. *F The first is additional, the second is a correction. *F *F \*A Tp(1;2)TE35B-SR302 *F \*i TE35B-SR302 *F \*i TE146(Z:SR100:GZ3)SR302 *F \*b 2-50.0 *F \*c 35B1-35B2 *F \*p homozygous viable; mutant for noc *F \*O spontaneous derivative of Tp(1;2)TE35B(Z:SR100)GZ3 *F \*p Eye color on a w{-} background genotype: red, not suppressed by z{1} *F \*s FB[w{+} rst{+}] *F \*x *F \# *F \*A Tp(1;2)TE35B-SR401 *F \*i TE35B-SR401 *F \*i TE146(Z:SR100)SR401 *F \*b 2-50.0 *F \*c 35B1-35B2 *F \*p homozygous viable; mutant for noc *F \*O spontaneous derivative of Tp(1;2)TE35B(Z)SR100 *F \*p Eye color on a w{-} background genotype: red, not suppressed by z{1} *F \*s FB[w{+}, rst{+}]; 4 polytene bands *F \*x *F *F John # *U FBrf0086249 *a Ashburner *b M. *c J. *d Roote *t 1996.3.6 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Mar 6 10:04:26 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 6 Mar 1996 09:59:36 +0100 *F To: Michael Ashburner *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Tp pk *F Content-Length: 146 *F *F DC17-8, Df spleD4 Tp(2;2)pk-sple22 41E-F;42F3;43A1 *F *F pk-sple[81h] Tp(2;2)pk-sple[26] CyO+29F;58E-58B;42A-42F;45A-42F;29F *F ------------ *F Michael adds: *F *F According to my cytology notes (#15466) the new order of pk-sple[26] *F (which was made by Daren Coulson on CyO) is: *F *F 21 - 22D1 | 33F5 - 30F | 50D1 - 58A4 | 42A2 - 34A1 | 22D2 - 29F | 58E *F - 58B1 | 42A3 - 42F | 45A - 42F | 29F - 30E | 50C10 - 45F | 58E - 60 # *U FBrf0086251 *a Roote *b J. *t 1995.10.19 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Thu Oct 19 15:05:46 1995 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 19 Oct 1995 14:59:59 +0100 *F To: ag24 *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: abs update *F Content-Length: 3386 *F X-Lines: 82 *F Status: RO *F *F >> 8) Any mention of dgl should be changed to esg *F >is this because they are now allelic? If they are distinct but adjacent, *F >surely I should either change x << dgl to esg, or change dgl << x to esg, *F >but not both. If they are allelic, have you a reference? *F esg is complex. Some alleles complement each other. But dgl (=T(Y;2)J165) *F is an esg allele by any criterion - it just took me a long time to *F discover. Ref - me, I guess! # *U FBrf0086252 *a Matthews *b K. *t 1996.2.2 *T personal communication to FlyBase *u *F From: Kathy Matthews, Indiana University *F To: FlyBase *F Subject: Deficiencies for 44D *F Date: 2 Feb 96 *F *F Background: These not-yet-published deficiencies in the Bloomington Stock Center collection need to be added to FlyBase. They are described in the manuscript *F 'Identification and genetic analysis of wunen, a gene guiding Drosophila melanogaster germ cell migration' by Nian Zhang, Jiaping Zhang, Yan Chang, and *F Ken Howard. The following information comes from a preprint of that manuscript provided to the stock center along with the stocks. *F *F Df(2R)H3C1 43F;44D *F Df(2R)H3D3 44D;44F4-5 *F Df(2R)H3E1 44D;44F12 *F These three were generated by X-ray induced excision of a P insertion in ptc - 'ptc[H3], a viable P[lac, w[+]] insert at ptc (J. Hooper, personal *F communication)' - so all should overlap at the 44D bp. *F *F Df(2R)wun-GL 45C8;45D8 *F This one was isolated from In(2LR)GR15[L]L2[R]. It fails to complement l(2)06736 and l(2)03659 as well as wun. # *U FBrf0086253 *a Stronach *b B. *t 1996.2.12 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed Feb 7 08:19:20 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 7 Feb 96 08:19:20 GMT *F To: eleanor@gen.cam.ac.uk *F Subject: Help FlyBase: Lim genes *F Content-Length: 2588 *F Dear Beth, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F In our genes file we have 6 LIM-only genes. *F Find shortened entries below: *F \*a Lim1 *F \*i DmLIM-1 *F \*x FBrf0079460 == Stronach et al., 1995, A. Conf. Dros. Res. 36: 321B *F \*x FBrf0067663 == Stronach et al., 1994, A. Conf. Dros. Res. 35 Addendum: 16 *F \*a Lim2 *F \*i DmLIM-2 *F \*i Mlp1 *F \*x Cell 79, 221--231 (1994) *F \*x FBrf0079460 == Stronach et al., 1995, A. Conf. Dros. Res. 36: 321B *F \*x FBrf0067663 == Stronach et al., 1994, A. Conf. Dros. Res. 35 Addendum: 16 *F \*g X81192 *F \*a Lim3 *F \*i DmLIM-3 *F \*x FBrf0079460 == Stronach et al., 1995, A. Conf. Dros. Res. 36: 321B *F \*x FBrf0067663 == Stronach et al., 1994, A. Conf. Dros. Res. 35 Addendum: 16 *F \*a Lmo *F \*i Rho: Rhombotin *F \*c 17C1--17C2 *F \*x FBrf0052759 == Boehm et al., 1990, Oncogene 5: 1103--1105 *F \*x Oncogene 11, 1283-1290 (1995) *F \*g X83037 *F \*g X54988 *F \*a Mlp60A *F \*g X91244 *F \*i Mlp1 *F \*x Cell 79, 221--231 (1994) *F \*a Mlp84B *F \*g X91245 *F I was hoping you would be able to help with three main queries concerning *F these genes. If you could also consult Dr Beckerle about these queries I *F would be very grateful (we have no entry for Beckerle in the FlyBase people *F directory). *F 1) *F On a previous cruise of the LIM genes I read this statement from the *F introduction of Zhu et al, 1995, Oncogene 11: 1283: *F 'Lmo is the agreed new name of the LMO (LIM-only) genes as decided at the *F 'Workshop on LIM proteins and LIM domains' (Strasbourg, 1995)'. *F On the basis of this have you renamed your genes Lim-1, Lim-2 or Lim-3 to *F have Lmo designations? If you are considering this please keep in mind *F that we already have Lmo as a FlyBase accepted gene symbol (included in *F the above list). *F 2) *F We know that Lim-2 and Mlp60A are the same gene (from ADRC 36: 321B and *F amino acid homology). We have not merged these genes yet as we are unsure *F what to name the merged gene. *F Prof Ashburner prefers the Mlp designation as it distinguishes these genes *F from the non-muscle LIM-only proteins. But the Lim-2 symbol should be *F used if we conform to the rules of precedence. But if you are using Lmo *F designations then we should use this. *F HELP! *F On the same lines do you know if Lim-1 or Lim-3 are homologous to Lmo (on *F the basis of protein homology and cytological location) or if either are *F homologous to Mlp84B? *F 3) *F The sequence record for Mlp60A and Mlp84B states Stronach B.E., Siegrist *F S.E., Beckerle M.C. (Unpublished). Do you have the details for this *F publication now, if so could you please give me the information? *F Many thanks for any help you can offer, *F Eleanor Whitfield *F FlyBase *F From Beth.Stronach@m.cc.utah.edu Thu Feb 8 19:41:18 1996 *F Date: Thu, 8 Feb 1996 12:34:40 -0700 (MST) *F From: Ellen Beth Stronach *F To: Eleanor Whitfield *F Subject: Re: Help FlyBase: Lim genes *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 2735 *F Dear Eleanor *F Sorry it took so long to reply but I wanted to converse with my advisor *F before writing back. *F Here's how we see it. *F 1) Lim1=DmLIM-1... we isolated this sequence based on homology to chicken *F zyxin. It has three C-terminal LIM domains. It maps to 102EF. We were *F not sure if it is the true zyxin homolog, so referred to it as DmLIM-1 (for *F the first LIM gene we isolated). We don't particularly like this *F designation and I think it would not be wise to call it LIM1 because it *F might get confused with vertebrate LIM-1's which are LIM-homeodomain *F proteins, clearly not homologs of this gene. So we have a dilemma, *F nothing is published formally yet about this gene, perhaps you can *F advise... should we call it drosophila zyxin, or LIM102EF for convention , *F or something else like ZRP:zyxin related protein. We have no expression *F data yet. *F 2) DmLIM-2=Lim2=Mlp1=Mlp60A... this gene has a single LIM domain and is *F clearly expressed in muscle. We prefer the Mlp designation. We submitted *F sequence under the name Mlp60A, thinking this was similar to established *F conventions for naming genes in Drosophila...e.g.Actins plus their map *F positions. Our competitors, however, published first in CELL 79, 221-231, *F and chose to call the gene Mlp1... we have submitted our own paper and call *F the gene Mlp1 deferring to their name...Again, please advise. We are not *F interested in keeping the 'Lim2' name. Also, this gene does contain only *F LIM domains but is clearly quite different from the LMO/Rhombotin family, *F so we prefer not to use the LMO designation. *F 3) DmLIM-3=Lim3=Mlp84B....This gene is very similar to Mlp1 in sequence, *F but it has five LIM domains and clearly maps to a different location, *F 84BC. We believe that it is a family member with Mlp1, and it is *F definitely also expressed in muscle. We have chosen to submit the sequence *F as Mlp84B, like Mlp60A. This gene is also described in our paper which is *F submitted and being reviewed right now. I suppose we could call it Mlp2, *F but this adds just another name, so what do you think??? Stick with *F Mlp84B, NOT Lim3 or DmLIM-3? Again, we do not feel the LMO designation is *F appropriate as this gene is related to Mlp1 not Rhombotin. *F Well, I hope this is helpful. We are willing and eager to work out a good *F naming system for these LIM genes according to established practice. If *F you need any further information, please don't hesitate to write again. *F and perhaps we can decide on something for the 4th chromosome gene that *F is related to vertebrate zyxin. *F Thanx for considering this issue with us and for your patience. *F Sincerely, *F Beth Stronach *F Dept of Biology *F University of Utah *F Salt Lake City, Utah 84112 *F (801) 585-6905 *F From eleanor@gen.cam.ac.uk Mon Feb 12 09:10:31 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 12 Feb 96 09:10:27 GMT *F To: Beth.Stronach@m.cc.utah.edu *F Subject: Re: Help FlyBase: Lim genes *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 4347 *F Hi Beth, *F In answer to each section: *F >1) Lim1=DmLIM-1... we isolated this sequence based on homology to *F >chicken zyxin. It has three c-terminal LIM domains. It maps to 102EF. *F >We were not sure if it is the true zyxin homolog, so referred to it as *F >DmLIM-1 (for the first LIM gene we isolated). We don't particularly like *F >this designation and I think it *F >would not be wise to call it LIM1 because it might get confused with *F >vertebrate LIM-1's which are LIM-homeodomain proteins, clearly not *F >homologs of this gene. So we have a dilemma, nothing is published *F >formally yet about this gene, perhaps you can advise... should we call it *F >drosophila zyxin, or LIM102EF for convention , or something else like *F >ZRP:zyxin related protein. We have no expression data yet. *F A prefix to indicate that the gene is from Drosophila, e.g. D, Dm, Dmel or *F Dro is redundant in the database and is, therefore, not used in valid gene *F names. If you are concerned that the symbol Lim1 suggests homology to *F vertebrate Lim-1's then using the cytology as a suffix would be *F acceptable. In later formal publications the valid name would be Lim102EF, *F this would be the valid symbol in FlyBase with the synonyms Lim1 and *F DmLim-1. Please note the nomenclature: genes named after a protein product *F begin with an uppercase letter, i.e Lim102EF NOT LIM102EF. *F Also, could you please confirm that this gene, that does contain only LIM *F domains, is different from Lmo and therefore that you should not use an Lmo *F designation? If it is a member of the Lmo family the valid symbol could *F be Lmo102EF? *F >2) DmLIM-2=Lim2=Mlp1=Mlp60A... this gene has a single LIM domain and *F >is clearly expressed in muscle. We prefer the Mlp designation. We submitted *F >sequence under the name Mlp60A, thinking this was similar to established *F >conventions for naming genes in Drosophila...e.g.Actins plus their map *F >positions. Our competitors, however, published first in CELL 79, *F >221-231, and chose to call the gene Mlp1... we have submitted our own *F >paper and call the gene Mlp1 deferring to their name...Again, please *F >advise. We are not interested in keeping the 'Lim2' name. Also, this *F >gene does contain only LIM domains but is clearly quite different from *F >the LMO/Rhombotin family, so we prefer not to use the LMO designation. *F At present we have both Lim2 and Mlp60A. Mlp60A was used over and above *F Mlp1 as we did not want to infer homology to the vertebrate Mlp1 gene, so *F you were correct in using the Mlp60A symbol. Based on your information we *F shall merge these genes and the valid symbol for both will be Mlp60A (as *F you and also Prof. Ashburner prefer), synonyms Lim2 and DmLIM-2. If it is *F not too late maybe you could change the designation from Mlp1 to Mlp60A in *F your submitted paper? *F >3) DmLIM-3=Lim3=Mlp84B....This gene is very similar to Mlp1 in sequence, *F >but it has five LIM domains and clearly maps to a different location, *F >84BC. We believe that it is a family member with Mlp1, and it is *F >definitely also expressed in muscle. We have chosen to submit the *F >sequence as Mlp84B, like Mlp60A. This gene is also described in our paper *F >which is submitted and being reviewed right now. I suppose we could call *F >it Mlp2, but this adds just another name, so what do you think??? *F >Stick with Mlp84B, NOT Lim3 or DmLIM-3? Again, we do not feel the LMO *F >designation is appropriate as this gene is related to Mlp1 not Rhombotin. *F I'm sure you will know the answer to your question now based on my previous *F comments, please use Mlp84B for the valid symbol (not Mlp2). In FlyBase we *F have both Lim3 and Mlp84B, I shall merge these genes and the valid symbol *F will be Mlp84B with synonyms DmLIM-3 and Lim3. *F The only outstanding point from my original communication, which I shall *F revise based on information already given, is: *F Do you know if Lim1 and Lmo belong to the same family and therefore Lim1 *F should have an Lmo designation? *F (I have asked this in the above text for point 1) *F Also I am assuming the paper Stronach B.E., Siegrist S.E., Beckerle M.C. *F (Unpublished) is that which is being reviewed as we speak and therefore you *F do not know the publication details yet? *F Considering the size of my original message I have to thank you (and your *F advisor) for a very thorough coverage and I eagerly await your reply! *F Many thanks. *F Eleanor Whitfield *F From Beth.Stronach@m.cc.utah.edu Mon Feb 12 20:25:14 1996 *F Date: Mon, 12 Feb 1996 13:13:56 -0700 (MST) *F From: Ellen Beth Stronach *F To: Eleanor Whitfield *F Subject: Re: LIM gene nomenclature *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1083 *F Well, I believe Lim102EF not to be related to Lmo/rhombotin aside from *F having LIM domains...that is, it is part of a huge superfamily of LIM *F proteins but is not a close family member of rhombotin/Lmo. *F So in other words, we don't wish to refer to this gene with an Lmo *F designation, and we hereafter refer to it as Lim102EF *F also, to clarify, we don't have any further publication details on the *F Stronach et al paper. But perhaps any day I will hear back, and would be *F happy to update you with the information, also I will take into *F consideration your suggestion to call the gene Mlp60AB instead of Mlp1 in *F the paper. *F Thanks again, Beth Stronach *F From eleanor@gen.cam.ac.uk Tue Feb 13 08:41:56 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 13 Feb 96 08:41:50 GMT *F To: Beth.Stronach@m.cc.utah.edu *F Subject: Re: LIM gene nomenclature *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 431 *F Hi Beth, *F Thanks for your prompt reply. *F I shall curate the new information you have given me as a personal *F communication from yourself and look forward to receiving the publication *F information on the new paper. We curate 75 journals, of which we have a *F top ten that we are concentrating on getting upto date. I imagine your *F paper will be in one of these journals. *F Eleanor Whitfield # *U FBrf0086254 *a Raftery *b L. *t 1995.4.6 *T personal communication to FlyBase *u *F Archived # *U FBrf0086255 *a Goriely *b A. *t 1996.2.27 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon Feb 5 13:57:36 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 5 Feb 96 13:56:55 GMT *F To: goriely@rockvax.rockefeller.edu *F Subject: Help FlyBase - Gsc location *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 478 *F Hi Dr Goriely, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F Prof Ashburner has noticed that the location of Gsc that you give in the *F sequence record X95420: *F FT /map='21C-D' *F differs from that given in this abstract: *F FBrf0055353 == Stella et al., 1992, A. Conf. Dros. Res. 33: 58 *F which is 60C-D. *F Could you please clear up this confusion by telling us the correct *F location? *F Many thanks, *F Eleanor Whitfield *F FlyBase *F From ag73@miranda.umds.ac.uk Tue Feb 27 14:55:32 1996 *F Date: Tue, 27 Feb 1996 14:49:05 +0000 (GMT) *F From: Dr Anne Goriely *F To: eleanor@gen.cam.ac.uk *F Subject: gsc location (X95420) *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 762 *F Hi Eleanor, *F Sorry for the delay about your letter, but I only got it today as I moved *F to Uk a month ago. Concerning the discrepancy between a previous *F publication and our submission to Flybase of D-gsc location, I can confirm *F that D-Gsc is located at position 21C5-6. The previous mapping by Stella et *F al. was wrong (just the wrong chromosome arm!). The ms concerning D-gsc is *F in press in Development (and will come out in June 1996), there is another *F paper from Jackle's lab which is currently being reviewed about the gene as *F well. *F If you need any other information, please contact me at my new *F address in *F Department of Developmental Neurobiology *F Guy's Hospital *F London Bridge *F London SE1 9RT *F 0171-955 5000 (x5928) *F a.goriely@umds.ac.uk *F Anne Goriely # *U FBrf0086256 *a Berg *b C. *t 1996.2.28 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Feb 27 10:48:09 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 27 Feb 96 10:48:02 GMT *F To: berg@genetics.washington.edu *F Subject: Help FlyBase - 0482 insertion *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 975 *F Dear Dr. Berg, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F I am curating your paper: *F \*x FBrf0078675 == Horowitz and Berg, 1995, Genetics 139(1): 327--335 *F in which you mention a pipsqueak P{PZ} insertion allele 0482. *F In our genes file the allele 0482 is a mutation of lola. *F symbol: lola *F name: longitudinals lacking *F location: 47A11--47A12 by in situ to lola[00642] *F allele: lola[00482] *F insertion: P{PZ}lola[00482] *F mutagen: P-element insertion *F discoverer: A. Spradling *F reference: FBrf0067338 == BDGP Project Members, 1994-, Berkeley *F Drosophila Genome Project *F symbol: psq *F name: pipsqueak *F location: 47B1--47B4 *F 47A9--47A10 *F You mention that 0482 chromosome has two insertions, the second insertion *F into a neighbouring lethal complementation group. Is this complementation *F group lola? If the gene is not lola then are lola and psq the same gene and *F the insertion hits another gene? *F Regards, *F Eleanor Whitfield *F FlyBase *F From berg@calypte.genetics.washington.edu Tue Feb 27 18:35:05 1996 *F Date: Tue, 27 Feb 96 11:16:09 PST *F From: 'Celeste Berg' *F Reply-To: 'Celeste Berg' *F To: eleanor@gen.cam.ac.uk *F Subject: Re: Help FlyBase - 0482 insertion *F Content-Length: 3076 *F Dear Eleanor: *F At the time we submitted that paper (July 1994), we did not know that the second *F insertion was in lola. We now have confirmation from Ed Giniger (who is right *F down the street at the Fred Hutchinson Cancer Research Center) that the second *F insertion IS in lola. *F As a matter of fact, I created a deletion by excising both P elements in 0482, *F thus deleting the DNA starting in the big intron of psk (47A10-12) and heading *F distally to the site of the second insertion, which Ed Giniger tells me maps *F between the two promoters for lola (47A13-16). (These are my and Ed's *F mappings... probably not as accurate as Allan's!) That deletion is called *F Delta(Greek symbol)18. Ed and our analyses of this deletion show that psk and *F lola are transcribed in opposite directions, psk toward the centromere. *F Although both genes have BTB domains, they are definitely not the same gene. *F We have a paper in review (still waiting to hear - it seems like ages now...) *F that describes this deletion but not in as much detail as I've provided for you. *F It also states that lola is a hotspot for insertion (there were 10 alleles from *F the Spradling lab screen). I did complementation tests with all of our psk *F alleles and all of these lola alleles and only 0482 failed to complement psk and *F lola. Its clear there were two hits. *F Celeste # *U FBrf0086257 *a Gelbart *b W.M. *t 1996.3.6 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Fri Mar 1 14:05:40 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 1 Mar 96 14:05:16 GMT *F To: gelbart@morgan.harvard.edu *F Subject: Help FlyBase - Mad *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1012 *F Hi Bill, *F I have three questions concerning your paper: *F \*x FBrf0080380 == Sekelsky et al., 1995, Genetics 139(3): 1347--1358 *F 1) *F This aberration: Df(2L)DTD62 2[P]3[D];H7 3[P]2[D] *F is mentioned in Table 1 on pg1349. *F We do not have DTD62, is it a T(2;3), what are its breakpoints, do you have *F a reference for it? Any other information you can provide would be *F marvellous too! *F We have T(2;3)H7 but the breakpoints you list do not match those we have in *F the database, are you revising the breakpoints or citing breakpoints from a *F previous paper, if so which one? *F 2) *F This aberration: Dp(2;3)JS20 *F is mentioned in Figure 1 on pg 1350. *F We do not have this aberration in the database and yet you fail to give any *F details, please tell me as much as possible. Previous reference would be *F yummy! *F 3) *F Transcript: c24 *F is mentioned in Figure 5 on pg1353. *F Was just wondering if you have done more work on this transcript and *F possibly given it a gene symbol yet? One less anon in the database if you *F have. *F Many thanks, *F Nellie *F From gelbart@morgan.harvard.edu Tue Mar 5 17:31:21 1996 *F Date: Tue, 05 Mar 1996 12:07:50 -0500 (EST) *F From: gelbart@morgan.harvard.edu (William Gelbart) *F Subject: Re: Help FlyBase - Mad *F To: eleanor@gen.cam.ac.uk *F Cc: gelbart@morgan.harvard.edu *F Content-Transfer-Encoding: 7BIT *F Content-Length: 3576 *F Hi Nellie, *F Here's the information you requested. *F Bill *F ======================================================================== *F T(2;3)DTD62 == T(2;3) 23F ; 80F *F Progenitor chromosomes: 2 == dpp[d-ho] *F 3 == unknown wild-type *F Mutagen: X ray *F Discoverer: Gelbart *F Recovered on the basis of pseudolinkage between chromosomes 2 and 3. *F Subsequently shown to disrupt transvection in T(2;3)DTD62, dpp[d-ho] *F / dpp[hr4] individuals (Gelbart, unpublished). *F ======================================================================== *F T(2;3)HVD7 == T(2;3) 23D1-2 ; 80F *F Synonym: T(2;3)H7 *F Progenitor chromosomes: 2 == dpp[d-ho] *F 3 == unknown wild-type *F Mutagen: X ray *F Discoverer: Gelbart *F Recovered as a heldout fly in an experiment to identify dpp transvection- *F disrupting rearrangements (DTDs). Upon retesting, did not display a DTD *F effect, and so it is given the suffix 'HVD' for the consonants in Harvard *F according to proper Bostonian pronunciation. Arose during the mutageneses *F described in Gelbart, 1982 (FBrf0038632). *F ======================================================================== *F Df(2L)DTD62-HVD7 == Ts(2R;3L)DTD62 + Ts(2L;3R)HVD7 *F New order: 2Lt - 23D | 80F - 3Rt *F 2Rt - 23F | 80F - 3Lt *F Deficient for: 23D1-2 ; 23F *F Deleted for: Mad *F Synonym: Df(2L)DTD62 2[P]3[D];H7 3[P]2[D] *F Mutagen: Synthetic *F Creator: Sekelsky *F Progenitors: T(2;3)DTD62 *F T(2;3)HVD7 *F Produced by segregation in a fly initially heterozygous for T(2:3)DTD62 *F and T(2;3)HVD7. *F ======================================================================== *F Dp(2;3)JS20 == Dp(2;3) 23C ; 23D ; 94D1-2 *F uDp *F Citation: FBrf0070678, pages 75-76 *F Title Mothers against dpp: a gene required for decapentaplegic *F function in Drosophila melanogaster. *F Author(s) Sekelsky, J.J. *F Year 1993 *F Journal Ph.D. Thesis, Harvard University, MA *F Pages ix + 152pp *F Type thesis *F Flybase ID FBrf0070678 *F Progenitor chromosomes: 2 == dpp[d-ho] Tp(1;2)TE23CD *F 3 == unknown wild-type *F Mutagen: gamma ray *F Discoverer: Sekelsky *F Recovered as a red-eyed fly among yellow-eyed z[1] w[11E4] ; dpp[d-ho] *F Tp(1;2)TE23CD, w[+] / dpp[d-ho] Tp(1;2)TE23CD, w[+] dp siblings. Red eyes *F are due to the unpaired w[+] gene inserted into 94D1-2. The reciprocal *F deletion segregant, if it arose, was not recovered. *F ======================================================================== *F >Transcript: c24 *F >is mentioned in Figure 5 on pg1353. *F >Was just wondering if you have done more work on this transcript and *F >possibly given it a gene symbol yet? One less anon in the database if you *F >have. *F Sorry, I can't help you on this one. Not only do we have nothing new on *F c24, but if I play things right, we never will. Studying this gene at all *F was just a by-product of honing in on the Mad gene. So, unless you want *F me to call it one of these 'next door gene' cutesy names, like Almost Mad, *F which I think would be absurd, the best thing to do is leave it wallowing in *F anon-imity, and live with its naffiness until someone comes along and actually studies the blessed gene. *F ======================================================================== # *U FBrf0086258 *a Gausz *b J. *t 1996.3.14 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Wed Mar 6 10:38:21 1996 *F From: Michael Ashburner (Genetics) *F Date: Wed, 6 Mar 96 10:34:10 GMT *F To: d_horowicz@omega.lif.icnet.uk *F Subject: Help FlyBase please *F Cc: ag24@gen.cam.ac.uk, rd120@gen.cam.ac.uk, gm119@gen.cam.ac.uk, *F eleanor@gen.cam.ac.uk, ma11@gen.cam.ac.uk *F Content-Length: 1279 *F David *F We are working with the Umea stock Centre (which, btw, now has EU *F funding !) to ensure that all of the gene/aberration/allele symbols *F they use are 'valid' FB names. This will mean that we can then link *F FB genes, alleles and abs directly to the stock list. *F As you can imagine this is not easy. Umea have the following from you *F which _appear_ not to be in FB - at least not under a name we *F can recognise. If you could give us further information on them *F we can create records for these. *F We would like to know: *F present name - if different *F any synonyms *F references - if any *F cytology limits *F genetic limits *F mutagen *F and anything else anyone else might find useful. *F We will curate yr reply as a Personal Communication to FlyBase. *F 58000. Df(3R)P-16 from Ish-Horowicz *F 58200. Df(3R)P-58 87A4,5-6;87A9,from Ish-Horowicz *F 58900. Df(3R)T-01 from Ish-Horowicz.Derived from transposing element TE86F. *F 59100. Df(3R)T-05 from Ish-Horowicz.Derived from transposing element TE86F. *F 59300. Df(3R)T-25 from Ish-Horowicz.Derived from transposing element TE86F. *F 59400. Df(3R)T-29 from Ish-Horowicz.Derived from transposing element TE86F. *F 59700. Df(3R)T-43 from Ish-Horowicz.Derived from transposing element TE86F. *F 65600. l(3)87A-02 from Ish-Horowicz 4/83. *F Many thanks *F Michael *F From GAUSZ@everx.szbk.u-szeged.hu Thu Mar 14 16:22:25 1996 *F From: 'Janos Gausz' *F Organization: Biological Research Center *F To: m.ashburner@gen.cam.ac.uk *F Date: Thu, 14 Mar 1996 17:15:35 MET *F Subject: stocks *F Priority: normal *F X-Mailer: Pegasus Mail for Windows (v2.01) *F Content-Length: 884 *F Dear Mike, *F Yes, I got your message. Unfortunately, I do not have any of *F these stocks. I hope they still exist in Umea. If I am correct P58 is *F an X-ray induced deficiency which was isolated on the basis of w+ *F loss of TE-98 in our laboratory. I do not know anything about P-16 *F but based on the name it has to come from the same experiment. *F All the other T-labeled stocks are X-ray derivatives of TE-28 but *F they were not characterized by cytology because none of them had a *F genetically unique break in the 87 region. That is all what I know. *F Otherwise I hope that everything is fine with you. *F Biological Research Center *F Hungarian Academy of Sciences *F Institute of Genetics *F H-6701 Szeged, POB 521 *F HUNGARY *F Phone : 36-62-432232 *F Fax : 36-62-433503 *F 36-62-432576 *F 36-62-433188 *F E-mail: Gausz@everx.szbk.u-szeged.hu # *U FBrf0086260 *a Holmgren *b R. *t 1996.1.26 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Jan 8 09:52:58 1996 *F Date: Mon, 8 Jan 96 09:52:09 GMT *F To: holmgren@casbah.acns.nwu.edu *F Cc: rd120@gen.cam.ac.uk, crosby@morgan.harvard.edu *F Dear Dr. Holmgren, *F I am writing about an FRT-Hsp70-polyA-FRT cassette that you made, *F described in: *F \*x FBrf0076140 == Wilder and Perrimon, 1995, Development 121(2): 477--488 *F To describe this construct accurately in FlyBase we need to know from which *F of the five Hsp70 genes (Hsp70Aa, Hsp70Ab, Hsp70Ba, Hsp70Bb, or Hsp70Bc) *F the polyA was isolated. Can you enlighten us? *F From holmgren@casbah.acns.nwu.edu Mon Jan 29 22:16:57 1996 *F Date: Mon, 29 Jan 1996 16:14:41 -0600 *F To: Rachel Drysdale (Genetics) *F The poly A site was taken from the intergene region (therefore it is the poly A site of the first hsp70 gene) of clone 132E3 from 87C1. The reference is Karch et al. (1981) JMB 148:219-230. # *U FBrf0086261 *a Noll *b M. *t 1996.3.2 *T personal communication to FlyBase *u *F From noll@molbio2.unizh.ch Date: Sat, 2 Mar 1996 11:39:22 +0100 *F To: Rachel Drysdale (Genetics) *F From: noll@molbio2.unizh.ch (Markus Noll) *F Subject: Re: helping FlyBase *F Content-Length: 1491 *F Dear Rachel, *F I have gone back to the records of my diploma student *F Roland Rutschmann who has isolated nearly ten years ago genomic *F clones encoding four prd-type homeodomains. One of them was mapped *F to 57B5,6. Although we have isolated a cognate cDNA, it appears *F that it was never sequenced. Since the genomic sequence encoding *F the homeodomain seems to be interrupted by an intron after amino *F acid 44 or, more likely, after amino acid 37,we have only a partial *F homeodomain sequence of this gene, which we called PPH17 at the time. *F This sequence is different from both 57San and orthopedia/W26: *F KKKHRRNRTTFTTYQLHELERAFRKSHYPDVYSREELAMK(VNLPRGS). *F I seem to remember that I have compared our sequence a long time ago *F with a homeodomain sequence of a gene that was isolated by Bill McGinnis *F and that also mapped to 57B5,6. If I remember correctly, his sequence *F turned out to be identical with our sequence. Perhaps he knows more *F about this gene. Also Uwe Walldorf had a homeodomain gene in this region *F whose sequence was however different from ours. *F With best regards, *F Markus # *U FBrf0086262 *a McGinnis *b W. *t 1996.3.4 *T personal communication to FlyBase *u *F Date: Mon, 4 Mar 1996 09:21:27 -0800 *F To: Rachel Drysdale (Genetics) *F From: mcginnis@jeeves.ucsd.edu (Willam McGinnis) *F Subject: Re: 57B homeodomains *F Rachel, *F Here's what I know about E97. We got a bit of 3' sequence from the *F homeobox region, but none that overlapped with the PPH17 sequence. In *F addition, the sequence was done only once and that sloppily, so was highly *F inaccurate . However, Dyana Dalton, a grad student in my lab, did cross *F hybridize Markus Noll's genomic clone containing PPH17 and our genomic *F clone containing the E97 homeobox. They didn't give a signal indicating *F sequence identity (besides a weak signal due to the homeobox homology), so *F we concluded that they are likely to be different homeobox loci in 57B1-5. *F Bill McGinnis # *U FBrf0086263 *a Nusslein-Volhard *b C. *t 1996.3.6 *T personal communication to FlyBase *u *F Date: Wed, 6 Mar 1996 17:24:48 +0100 *F To: Rachel Drysdale (Genetics) *F From: cnv@mailer.mpib-tuebingen.mpg.de (Christiane Nuesslein-Volhard) *F Subject: Re: helping FlyBase *F Dear Dr Drysdale, the mutant Puff=E4rmel has never been described properly *F and therefore can not be in the fly base. It is a dominant with some wing *F duplication, the penetrance depends on background. It may be an allele of *F paired. *F Christiane Nuesslein-Volhard # *U FBrf0086264 *a Glover *b D.M. *t 1996.3.6 *T personal communication to FlyBase *u *F Date: Wed, 6 Mar 1996 15:55:10 +0000 (GMT) *F From: 'D.M. Glover Anatomy and Physiology ext 4793 ' *F To: ma11 *F Subject: Re: Help FlyBase *F Michael *F three rows is between 54F and 55A1 *F David # *U FBrf0086265 *a Roote *b J. *t 1996.3.6 *T personal communication to FlyBase *u *F Date: Wed, 6 Mar 1996 09:59:36 +0100 *F To: Michael Ashburner *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Tp pk *F DC17-8, Df spleD4 Tp(2;2)pk-sple22 41E-F;42F3;43A1 *F pk-sple[81h] Tp(2;2)pk-sple[26] CyO+29F;58E-58B;42A-42F;45A-42F;29F *F \------------ *F Michael adds: *F According to my cytology notes (#15466) the new order of pk-sple[26] *F (which was made by Daren Coulson on CyO) is: *F 21 - 22D1 | 33F5 - 30F | 50D1 - 58A4 | 42A2 - 34A1 | 22D2 - 29F | 58E *F - 58B1 | 42A3 - 42F | 45A - 42F | 29F - 30E | 50C10 - 45A | 58E - 60 # *U FBrf0086266 *a Baker *b N. *t 1996.3.7 *T personal communication to FlyBase *u *F Date: Thu, 07 Mar 1996 11:42:59 -0500 *F From: baker@aecom.yu.edu (Dr. Nicholas Baker) *F Subject: If/Kr *F To: flybase-updates@morgan.harvard.edu *F The Irregular facets mutation (If: 2-108; 60F3) causes misexpression of Kr *F protein in the eye disc and is probably allelic to Kr. See data in Baker *F et al. J. Neurogenet. vol.8 p 85-100, 1992. # *U FBrf0086267 *a Sanchez-Herrero *b E. *t 1996.3.7 *T personal communication to FlyBase *u *F Archived # *U FBrf0086268 *a Dickson *b B. *t 1996.3.29 *T personal communication to FlyBase *u *F Date: Fri, 29 Mar 1996 11:00:00 +0100 *F To: Rachel Drysdale (Genetics) *F From: barry@zool.unizh.ch (Barry Dickson) *F Dear Rachel, *F In my nomenclature for transgenic lines, 1010T2 and 1010T10 represent *F different insertions (T2 and T10) of the same plasmid (pBD1010). These *F numbers are therefore quite meaningless to anyone but me, but since I have *F unfortunately distributed these stocks to several people using these *F designations, I guess they will have to stick. *F Thanks, and best wishes, *F Barry *F >I am curating the genetic information about these constructs which you *F >kindly deposited with Kath in the stock center. To be sure we get this *F >right, could you confirm that 1010T[[2]] and 1010T[[10]] are insertion *F >identifiers, as opposed to a description of something that is inside the *F >transposon? (or otherwise set us straight!) *F > *F >> 1010T[[2]]: UAS-GFP[[S65T]], pCaSpeR3, II.chromosome homozygous viable *F >> insert, construct and transgene done by Barry Dickson (unpublished) *F >> using the GFP[[S65T]] mutant [Heim, R. and Tsien, R.Y. (1996) Current *F >> Biology 6, 178-182]. *F >> *F >> 1010T[[10]]: UAS-GFP[[S65T]], pCaSpeR3, III.chromosome homozygous viable *F >> insert, construct and transgene done by Barry Dickson (unpublished) *F >> using the GFP[[S65T]] mutant [Heim, R. and Tsien, R.Y. (1996) Current *F >> Biology 6, 178-182]. *F > *F >with best wishes, *F > *F >Rachel *F > # *U FBrf0086743 *a Roote *b J. *t 1996.4.17 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Apr 17 18:55:41 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 17 Apr 1996 18:50:45 +0100 *F To: Michael Ashburner (Genetics) *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: help FlyBase please *F Content-Length: 339 *F *F >John *F >You sent Kathy these - what is their origin etc ? *F >M *F ># *F >*a Df(2R)GJ66-47 *F >*a Df(2R)GJ68-36 *F *F Michael, *F *F Df(2R)GJ66-47, FRT42D cn sp/CyO (=Stan1) 46D7-9;47F15-16 *F *F Df(2R)GJ68-36, FRT42D cn sp/CyO (=Stan2) 46F1-2;47D1-2 *F *F Made by Glynnis, X-ray, Simon's cytology. *F *F The names Stan1 and Stan2 have been agreed by Adler. *F *F John # *U FBrf0086744 *a Dearolf *b C.R. *t 1996.5.2 *T personal communication to FlyBase *u *F Fax from C.R. Dearolf to M. Ashburner May 2 1996. *F Copy available from FlyBase. # *U FBrf0086745 *a Nambu *b J.R. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086746 *a Nakamura *b A. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0086747 *a Adler *b P. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086748 *a Kimbrell *b D.A. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086749 *a Jakubowski *b J.M. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086750 *a Rogina *b B. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086751 *a Kozopas *b K. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086752 *a White-Cooper *b H. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086753 *a de Nooij *b J.C. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086754 *a Doerflinger *b H. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086755 *a Knirr *b S. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0086756 *a Noselli *b S. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086757 *a Werner *b L.A. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086758 *a Morcillo *b L.A. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086759 *a Coyle-Thompson *b C. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0086760 *a Anne *b J. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086761 *a Butler *b S. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086762 *a Carney *b G. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086763 *a Carrera *b P. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086764 *a Conley *b C. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0086765 *a Dimlich *b D. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086767 *a Fattah *b M. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086768 *a Galewsky *b S. *c A. *d Scherer *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086769 *a Gellon *b G. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086770 *a Guichet *b A. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086771 *a Herman *b T. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086772 *a Ito *b H. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086773 *a Kiger *b A. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086774 *a Li *b C. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086775 *a Mardahl-Dumesnil *b M. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086776 *a McCormack *b A. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086777 *a Megraw *b T.L. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086778 *a Merli *b C. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086779 *a Rastelli *b L. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086780 *a Renn *b S. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086781 *a Richards *b S. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086782 *a Rodriguez *b A. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086783 *a Roulier *b E. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086784 *a Scully *b A. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086785 *a Sink *b H. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0086786 *a Sun *b Y. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086787 *a Tang *b T. *t 1996.4.29 *T personal communication to FlyBase *u *F Archived # *U FBrf0086788 *a Terracol *b R. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived # *U FBrf0086789 *a Zhang *b P. *t 1996.4.28 *T personal communication to FlyBase *u *F Archived # *U FBrf0086796 *a Katz *b F. *t 1996.5.13 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon May 13 10:58:56 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 13 May 96 10:58:52 BST *F To: katz@utsw.swmed.edu *F Subject: Help FlyBase - fj enhancer trap *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 474 *F Dear Dr Katz, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F I am curating your paper: *F \*x FBrf0084478 == Villano and Katz, 1995, Development 121(9): 2767--2777 *F in which you discuss the expression of an fj enhancer trap. Could you *F please tell me the insertion line designation for this enhancer trap. This *F information is essential to capture the expression pattern for the enhancer *F trap. *F Many thanks *F Eleanor Whitfield *F FlyBase *F From KATZ@utsw.swmed.edu Mon May 13 15:54:09 1996 *F Date: Mon, 13 May 1996 09:45:55 -0500 (CDT) *F From: KATZ@utsw.swmed.edu *F Subject: Re: Help FlyBase - fj enhancer trap *F To: eleanor@gen.cam.ac.uk *F X-Vms-To: IN%'eleanor@gen.cam.ac.uk' *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 167 *F Our designation for the fj enhancer trap (for curation of the paper you *F designate FBrf0084478) is 9-IIw+. It is an insertion of PlacW. *F Sincerely yours, *F Flora Katz # *U FBrf0086797 *a Roote *b J. *t 1996.5.20 *T personal communication to FlyBase *u *F From cesunkel@mail.telepac.pt Thu Jan 25 22:21:56 1996 *F Date: Thu, 25 Jan 96 22:52:30 PST *F Reply-To: cesunkel@mail.telepac.pt (claudio sunkel) *F From: cesunkel@mail.telepac.pt (claudio sunkel) *F To: M.ASHBURNER@gen.cam.ac.uk *F Subject: 35B *F Content-Length: 1790 *F Dear Mike, *F We have a P mutant that has a mitotic phenotype that is uncovered *F both by Df(2L)A220 35B01-02;35B09 and Df(2L)35B01-03;35BC04-05. *F The stock appears to have two insertions very close to one *F another. One at 35B and the other at 35C. The one we are *F interested is in 35B since that is lethal over Df(2L)A220 and has *F a mitotic phenotype. We cloned one side of the insertion at 35B by *F reverse PCR and have some 500 pb of sequence from a region some 1 *F kb away from the P-insertion. The reason why it is at some *F distance from the end of the P is complicated but we are sure that *F is the insertion site because it has internal P sequences as we *F expected from a very rough idea of the transposon and also by in *F situ using the cloned PCR product. These stocks *F were made by J.M.Dura but I have not been able to find him. Any *F way since we have the sequence I thought I could send you the P *F stock as well as the sequence to compare against the sequence that *F you have over the whole region. We have also isolated genomic *F clones and are in the process of screening a cDNA library. If the *F sequence matches somewhere in your sequence it would be great. It *F might even be possible to find right away what kind of gene is *F mutated. Can you do the search and let me know the results ?? *F Please let me know about all this and I will send you both the *F sequence by email and the p-stock. I cannot send you the seq at *F the moment because I am writing from home but I will do it *F tomorrow. *F Bye *F Claudio *F \****************** *F From cscariol@ncc.up.pt Tue Jan 30 08:50:24 1996 *F Date: Tue, 30 Jan 1996 09:44:14 +0100 *F From: Claudio Enrique Sunkel Cariola *F Subject: corrections *F To: flybase-updates@morgan.harvard.edu *F 1) There is an error in the map for pP{>w[hs]>} (FBmc0000756) and the *F associated transposon. Segment 7 is listed as being ca. 7070 bp, which *F should be ca. 707 bp. The sequence looks to be correct. *F 2) The entry for mei-41 (FBgn0004367) references the mapping to Banga et *F al., 1986 Chromosoma (FBrf0043953), but this reference does not deal with *F mei-41. Rather, the gene was mapped to 14B13 - 14D1,2 by Mason et al., *F 1981 Mutation Research (FBrf003713) and subsequently to 14C4-6 by Banga et *F al., 1995 MGG (FBrf0079877). *F 3) For homologs of MEI-41: There is a more complete sequence for ATM *F under GenBank Acc No U33841. There is a newly discovered human gene more *F similar to MEI-41, called FRP1 (U49844). The homolog in S. cerevisiae is *F Mec1 (U31109). *F 4) Now that Mad (FBgn0011648) is becoming so hot, the two most important *F references should be added to the gene entry: Sekelsky, 1993 thesis *F (FBrf0070768) and Sekelsky et al., 1995 Genetics (FBrf0080380). Both of *F these list the phenotype in detail, describe the cloning, give C. elegans *F homologs, and describe a dozen mutant alleles phenotypically, and for *F three molecularly. *F Thanks! *F Jeff # *U FBrf0086810 *a Kalionis *b B. *t 1996.5.7 *T personal communication to FlyBase *u *F Date: Tue, 7 May 1996 12:53:07 +0930 *F To: rd120@gen.cam.ac.uk *F From: BillK *F Subject: homeodomains and wombats *F Dear Rachel, *F Prof. Rob Saint asked me to reply to your email regarding the Flybase *F information *F I have independently cloned a homeobox gene, called bk24, identical in amino *F acid sequence within the homeodomain to W26. Like W26, the gene maps at 57B *F and is expressed in the hindgut. *F The reference for this paper is Kalionis, B. and O'Farrell, P.H. (1993) *F Mechanisms of Development 43, 57-70 *F In this paper you will also find the homeodomain sequences of wom (bk50) and *F emu (bk36) neither of them are related to W26. The chromosome locations, *F expression patterns and cDNA sequences of wom and emu are unpublished. *F My clone bk24, which is described in the above paper is identical at the DNA *F and amino acid sequence level to W26 in the homeodomain. I checked this with *F Walter Gehring several years ago. I confirmed that bk24 locates to 57B. *F Please let me know if you need further details. *F Regards, *F Dr. Bill Kalionis # *U FBrf0086811 *a Bray *b S. *t 1996.5.13 *T personal communication to FlyBase *u *F Date: Mon, 13 May 1996 12:13:41 +0100 *F To: Rachel Drysdale (Genetics) *F From: sjb32@mole.bio.cam.ac.uk (Sarah Bray) *F Subject: Re: helping FlyBase *F Dear Rachel, *F The only two I really know much about are grh 300 and thr. I did at one *F time have some correspondence with Rick and have made some notes below from *F that. I got two alleles from Umea, grh300 and grh370 so the details are *F below. *F >present name -grh300, grh370 *F >any synonyms-Elf-1, NTF-1, l(2)IM45 *F >references - we have a manuscript in preparation about grh370, which is larval/pupal lethal (with a few escapers). grh300 is embryonic lethal. *F >cytology limits-54F *F >genetic limits *F >mutagen-EMS *F >and anything else anyone else might find useful- can I update this section when we've finished the paper? We may have things to add . *F > *F >39762.grh[300(II)] grh 300 prox. from Rick Tearle-89 *F I hope this is of some help!!! Let me know if you want any more *F clarification. *F Best wishes, Sarah. # *U FBrf0086812 *a Tearle *b R. *t 1996.5.14 *T personal communication to FlyBase *u *F Archived # *U FBrf0086813 *a Engels *b W. *t 1996.5.15 *T personal communication to FlyBase *u *F Date: Wed, 15 May 1996 15:21:09 -0500 *F From: wrengels@facstaff.wisc.edu (Bill Engels) *F Subject: l(2)04615 and mam *F To: flybase-updates@morgan.harvard.edu *F Dear FlyBase, *F We have been working with a P element insertion named P#1383. This had *F been previously listed as defining a lethal named l(2)04615. I noticed that *F you have recently removed this lethal locus and called it an allele of *F mastermind, calling it mam[04615]. *F Our data confirm that P#1383 fails to complement mam. However, we have *F other lethals in the region that complement mam but fail to complement *F P#1383 ( FBrf0085771). Therefore, we propose that l(2)04615 be reinstated *F as a locus rather than an allele of mam. It seems that P#1383 actually *F knocks more than one locus, and is therefore probably not a simple *F insertion. *F Bill Engels *F \\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ William R. Engels : WREngels@facstaff.wisc.edu *F Genetics Department, 445 Henry Mall : office: (608) 263-2213 *F University of Wisconsin : lab: (608) 262-5578 *F Madison, WI 53706 : FAX: (608) 262-2976 *F http://www.wisc.edu/genetics/CATG/engels/ *F \---------------------------------------------------------------------- # *U FBrf0086814 *a Walldorf *b U. *t 1996.5.20 *T personal communication to FlyBase *u *F Archived # *U FBrf0086815 *a Clark *b D. *t 1996.6.15 *T personal communication to FlyBase *u *F >From nobody Wed Jun 19 18:06:51 1996 *F Date: Wed, 19 Jun 96 18:06:49 EDT *F From: clarkd@unb.ca (Denise Clark) *F Subject: sequence transposon pP{DM23} *F To: flybase-help@morgan.harvard.edu *F Content-Length: 822 *F *F clarkd@unb.ca (Denise Clark) sent the following *F comments to flybase-help: *F *F ------------------------------------------------------------ *F A sequence is inverted: i.e.the 7.2 kb HindIII rosy fragment *F is inverted (1-7288). The construct report states the *F progenitor is assumed to be pP{Car30A}. It must be *F pP{Car30B} (Mismer and Rubin 1987 just say Carnegie 30). *F I have two reasons to believe this: *F *F 1)SalI + XhoI digest gives 3 + 8 kb bands, *F which is what you'd predict for a Car30B *F derivative of DM23 *F *F 2) I made a primer from the rosy sequence *F and one from the fragment I inserted into the *F mcs. I got a PCR product which could only be *F explained with a Car30B derivative. *F ------------------------------------------------------------ *F Server protocol: HTTP/1.0 *F Remote host: 131.202.97.10 *F Remote IP address: 131.202.97.10 *F *F >From crosby Thu Jun 20 10:43:09 1996 *F *F Dear Denise, *F *F Thanks for the information concerning P{DM23}. This is exactly the *F kind of input we had hoped to get from the fly community. Do you *F have any objection to our retaining your e-mail message as a personal *F communication that we can cite in the pP{DM23} and P{DM23} reports? *F *F Thanks again for taking the time to contact us. *F *F Sincerely, *F *F Lynn Crosby *F FlyBase *F *F >From clarkd@unb.ca Thu Jun 20 12:04:44 1996 *F *F Dear Lynn, *F *F I have no objection to you using my e-mail message. Glad to be of help. *F Its so nice to have all those vector sequences compiled! No more cutting *F and pasting from Genbank sequences to put it together ourselves! *F *F Denise # *U FBrf0086856 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu May 9 15:12:57 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 9 May 96 15:12:52 BST *F To: lgvolk@weizmann.weizmann.ac.il *F Subject: Help FlyBase - P1749 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 571 *F *F Dear Dr. Volk, *F *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F *F FlyBase minimally curates information from the ADRC abstract book and we *F pay particular attention to new genes/alleles etc that are described. Your *F abstract (17B) discusses a new gene, P1749. *F We have noticed that P1749 is the Bloomington stock number for l(3)10567 *F (at cytological location 64F). Is this the line you used or is P1749 something *F completely different? If it is something different could you please tell *F me more. *F *F HELP!! *F Eleanor Whitfield *F FlyBase *F *F From lgvolk@weizmann.weizmann.ac.il Sun May 12 08:11:57 1996 *F *F Dear Eleanor, *F Yes P1749 is the P element from the Spardling collection l(3)10567 which is *F mapped to 64F. We confirmed this localization by chromosomal in situ with *F rescued genomic DNA. *F Best regards, *F Talila. # *U FBrf0086858 *a Volk *b T. *t 1996.5.12 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu May 9 15:12:57 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 9 May 96 15:12:52 BST *F To: lgvolk@weizmann.weizmann.ac.il *F Subject: Help FlyBase - P1749 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 571 *F *F Dear Dr. Volk, *F *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F *F FlyBase minimally curates information from the ADRC abstract book and we *F pay particular attention to new genes/alleles etc that are described. Your *F abstract (17B) discusses a new gene, P1749. *F We have noticed that P1749 is the Bloomington stock number for l(3)10567 *F (at cytological location 64F). Is this the line you used or is P1749 something *F completely different? If it is something different could you please tell *F me more. *F *F HELP!! *F Eleanor Whitfield *F FlyBase *F *F From lgvolk@weizmann.weizmann.ac.il Sun May 12 08:11:57 1996 *F *F Dear Eleanor, *F Yes P1749 is the P element from the Spardling collection l(3)10567 which is *F mapped to 64F. We confirmed this localization by chromosomal in situ with *F rescued genomic DNA. *F Best regards, *F Talila. # *U FBrf0087762 *a Vigue *b C.L. *t 1978 *T personal communication to FlyBase *u Procedure for the quantitative assay of enzymatic ectivity. *F From eleanor@gen.cam.ac.uk Thu May 9 15:12:57 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 9 May 96 15:12:52 BST *F To: lgvolk@weizmann.weizmann.ac.il *F Subject: Help FlyBase - P1749 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 571 *F Dear Dr. Volk, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F FlyBase minimally curates information from the ADRC abstract book and we *F pay particular attention to new genes/alleles etc that are described. Your *F abstract (17B) discusses a new gene, P1749. *F We have noticed that P1749 is the Bloomington stock number for l(3)10567 *F (at cytological location 64F). Is this the line you used or is P1749 something *F completely different? If it is something different could you please tell *F me more. *F HELP!! *F Eleanor Whitfield *F FlyBase *F From lgvolk@weizmann.weizmann.ac.il Sun May 12 08:11:57 1996 *F Dear Eleanor, *F Yes P1749 is the P element from the Spardling collection l(3)10567 which is *F mapped to 64F. We confirmed this localization by chromosomal in situ with *F rescued genomic DNA. *F Best regards, *F Talila. # *U FBrf0087764 *a Volk *b T. *t 1996.5.12 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu May 9 15:12:57 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 9 May 96 15:12:52 BST *F To: lgvolk@weizmann.weizmann.ac.il *F Subject: Help FlyBase - P1749 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 571 *F Dear Dr. Volk, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F FlyBase minimally curates information from the ADRC abstract book and we *F pay particular attention to new genes/alleles etc that are described. Your *F abstract (17B) discusses a new gene, P1749. *F We have noticed that P1749 is the Bloomington stock number for l(3)10567 *F (at cytological location 64F). Is this the line you used or is P1749 something *F completely different? If it is something different could you please tell *F me more. *F HELP!! *F Eleanor Whitfield *F FlyBase *F From lgvolk@weizmann.weizmann.ac.il Sun May 12 08:11:57 1996 *F Dear Eleanor, *F Yes P1749 is the P element from the Spardling collection l(3)10567 which is *F mapped to 64F. We confirmed this localization by chromosomal in situ with *F rescued genomic DNA. *F Best regards, *F Talila. # *U FBrf0087819 *a Thummel *b C. *t 1996.8.7 *T personal communication to FlyBase *u *F >From cthummel@qmserver.genetics.utah.edu Thu Jun 20 12:27:46 1996 *F Date: 20 Jun 1996 10:26:13 U *F From: 'CThummel' *F Subject: pCaSpeR vectors *F To: 'Madeline Crosby' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 905 *F *F REGARDING pCaSpeR vectors *F *F Dear Lynn, *F You may remember that you contacted me a year ago regarding the sequences of P *F element vectors that we made, together with Vince Pirrotta. We have been *F working on those sequences (very slowly!) and finally have some accession *F numbers for you. We also have the maps of these vectors (and accession *F numbers) on a WWW page *F (http://www-hhmi.genetics.utah.edu/thummel/pelement.html) in case you are *F interested. The numbers are as follows: *F pCaSpeR EMBL 81644 *F pCaSpeR2 GenBank U59054 *F pCaSpeR3 GenBank U59055 *F pCaSpeR4 EMBL 81645 *F pCaSpeR-hs GenBank U59056 *F pCaSpeR-hs/act GenBank U60735 *F pCaSpeR hs43 lacZ EMBL 81643 *F I hope that this helps! We will let you know if we find the energy to *F assemble sequences of any lacZ vectors. I think it would be nice to have *F pCaSpeR-AUG-bgal, but I can't get too excited about it right now! *F Best wishes, *F Carl Thummel *F *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From crosby Thu Jun 20 15:35:54 1996 *F Date: Thu, 20 Jun 96 15:35:49 EDT *F From: crosby (Madeline Crosby) *F To: cthummel@qmserver.genetics.utah.edu *F Subject: Re: pCaSpeR vectors *F Cc: crosby *F Content-Type: X-sun-attachment *F Content-Length: 29725 *F *F *F Carl, *F *F Many thanks for remembering my request! Your timing could not be *F better: FlyBase is planning on publishing maps of commonly used *F vectors, enhancer traps, etc., in an upcoming FlyBase issue of DIS. *F I am not familiar with the details on permission from authors, etc., *F but I hope we can include these vectors. *F *F In regard to the lacZ vectors: I already have essentially complete *F compiled sequences for these. (Complete in the case of CaSpeR-betagal; *F a few unknown nucleotides in the case of CaSpeR-AUG-betagal.) Would you *F like to look them over and see what you think (attached below)? I know *F that Bill would like to work out a system of joint submissions to GenBank; *F do you think this might be a reasonable option for these two vectors? *F *F Another question: below is a comment I have included in my compilation *F of the white sequences in the CaSpeR vectors. Do you know anything *F about these discrepancies? *F *F ' Sequence discrepancies (+1 bp at 2176; c to t at undetermined position) *F in sequence entry listed versus white (sequence entry X02974 *F [Genbank / EMBL] | 10873 | 22 Feb 1993). FlyBase-compiled sequence is *F based upon the latter and thus differs from sequence in entry X81644.' *F *F *F Thanks again. *F *F *F Sincerely, *F *F Lynn *F *F Lynn Crosby *F FlyBase *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From cthummel@qmserver.genetics.utah.edu Fri Jun 21 17:39:38 1996 *F Date: 21 Jun 1996 15:37:25 U *F From: 'CThummel' *F Subject: Re: pCaSpeR vectors *F To: 'Madeline Crosby' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Type: x-binhex4 *F X-Attachments: 'Casper sequences' (type: binhex4) *F Content-Length: 34414 *F *F RE>>pCaSpeR vectors 6/21/96 *F *F Hi Lynn, *F My, you have been busy! Needless to say, I did not expect that anyone would *F put the time and effort necessary into the compilation of these sequences, *F much less someone who had not actually made the clones themselves! There are *F a lot of little cloning tricks in there that are tricky to figure out - even *F for me!! Anyhow, the basic sequences you sent look OK. There are, however, a *F few problems. First, the length of both vectors is off by several kb - they *F should each be about 12 kb total. The sequence you are missing is in the *F Casper vector. You should replace your Casper sequences in any case, since *F there have been several recent small changes that I learned about from Vince *F Pirrotta. I have enclosed the most recent Casper sequence, along with a *F modified version of your Casper-bgal vector. You had a run of 4 Cs downstream *F from the polylinker at the 5' end of lacZ - this should have been 3 Cs. The *F sequence was correct in your Casper-AUG-bgal sequence (probably because there *F you could see that the Adh AUG needed to be in frame with lacZ). Otherwise, *F the only parts I could not confirm were the Casper-SV40, SV40-lacZ junctions *F in both sequences, and Casper-Adh-lacZ junction in Casper-AUG-bgal. I think *F it would be safest to sequence through these junctions. Did you actually do *F that, or is your sequence just a best guess based on the literature? Some of *F these junctions are impossible to predict without sequencing. We would be *F happy to do the sequencing, if you don't mind waiting a bit. Please let me *F know what you think! Thanks for all your help getting this together. *F Best wishes, *F Carl *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From crosby Fri Jun 21 18:12:49 1996 *F Date: Fri, 21 Jun 96 18:12:43 EDT *F From: crosby (Madeline Crosby) *F To: cthummel@qmserver.genetics.utah.edu *F Subject: Re: pCaSpeR vectors *F Cc: crosby *F Content-Length: 2372 *F *F *F Carl, *F *F We're making progress! *F *F >First, the length of both vectors is off by several kb - they *F >should each be about 12 kb total. *F *F The CaSpeR vector sequences are not included in these -- they *F are the transposon-only versions. A slightly cumbersome but *F occasionally useful convention we have adopted is to *F represent everything (except construction intermediates) in *F two ways: both the plasmid (or cosmid) form and the transposon *F form. I hesitated to send you the plasmid form because it is *F less obvious which is the best orientation and what is the *F best starting point. *F *F The sizes I have currently listed are: *F *F pP{CaSpeR-betagal} 11.978kb *F P{CaSpeR-betagal} 9.218kb *F *F pP{CaSpeR-AUG-betagal} ~12.126kb *F P{CaSpeR-AUG-betagal ~9.366kb *F *F I know the ~ with all significant digits looks a bit strange, but *F the size is derived automatically by adding up component segments. *F *F *F > ...is your sequence just a best guess based on the literature? *F *F Yes. *F *F >Some of these junctions are impossible to predict without sequencing. *F *F Very true! Initially, I was trying to give some sort of confidence *F rating on the compiled sequences, but that really isn't practical. *F For things that are REALLY dubious, I include disclaimers in the *F comments sections. It might be a good idea to include some sort of *F generic disclaimer, along with a request for corrections when errors *F are discovered. *F *F *F >We would be happy to do the sequencing, if you don't mind waiting a bit. *F *F That would be terrific! Presumably these small stretches can be *F done relatively quickly. *F *F *F >You should replace your Casper sequences in any case, since *F >there have been several recent small changes that I learned about from Vince *F >Pirrotta. *F *F Is Vince going to correct his GenBank/EMBL entries? Large stretches of *F sequence like this I have been pulling from GenBank; we store only the *F compiled sequences and these are re-compiled periodically to reflect any *F changes that have been submitted to GenBank. With only a few exceptions, *F the only original sequence that we store is that of junctions. *F *F *F Thanks for all the input. Let me know how the sequencing goes. *F *F --Lynn *F *F *F P.S. I get the distinct impression that you haven't looked at *F TransposonSearch on FlyBase. It can only be accessed through the *F Web, but if you can manage to take a look at what we've been doing, *F I would really appreciate your feedback. *F *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From cthummel@qmserver.genetics.utah.edu Mon Jun 24 12:00:49 1996 *F Date: 24 Jun 1996 09:59:42 U *F From: 'CThummel' *F Subject: Re: pCaSpeR vectors *F To: 'Madeline Crosby' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 4066 *F *F RE>>pCaSpeR vectors 6/24/96 *F *F Hi Lynn, *F Thanks for your response. I have browsed through Transposon search and am *F very impressed. I must confess that although I use FlyBase several times each *F day, I have not tried that handy little window! Why don't you go ahead and *F submit your sequences with a disclaimer. We will then go ahead and sequence *F the junctions that I mentioned to you earlier. Although it should be quick, *F it won't be. We have a bunch of other stuff going on and will fit these *F sequencing reactions in as we can. I will let you know once we have assembled *F a complete corrected sequence for both Casper-bgal and Casper-AUG-bgal. *F Thanks again, *F Carl *F P.S. Vince will be correcting his EMBL submissions, so your re-compilation of *F the sequence should incorporate his changes. Keep up the terrific work! *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From cthummel@qmserver.genetics.utah.edu Mon Jul 29 16:50:12 1996 *F Date: 29 Jul 1996 14:51:15 U *F From: 'CThummel' *F Subject: Casper-bgal sequences *F To: 'Lynn Crosby' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 19532 *F *F REGARDING Casper-bgal sequences *F *F Hi Lynn, *F We have finished sequencing the junctions on pCasper-bgal and *F pCasper-AUG-bgal. We did the lacZ-SV40 junctions on both vectors, the *F SV40-Casper junctions on both vectors, and the Casper-AUG-lacZ junctions on *F pCasper-AUG-bgal. As expected, there were small insertions and/or deletions *F to make in these regions. I have enclosed the modified sequences. I hope *F that these help! Please send me the accession numbers once you submit them to *F GenBank. Thanks again for your help in getting these sequences together! *F Best wishes, *F Carl *F *F *F <<<<<< Attached TEXT file named 'cas-aug-bgal' follows >>>>>> *F catgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgct *F tgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtggaataaaaaaaaatgaaata *F ttgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgccgaagtgtgctattaagagaaaattgtggg *F agcagagccttgggtgcagccttggtgaaaactcccaaatttgtgatacccactttaatgattcgcagtggaaggctgca *F cctgcaaaaggtcagacatttaaaaggaggcgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccaga *F aaagataaaagaaggctataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaaca *F ataacgtgactgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagta *F cgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaattaaatgtattc *F ttattattatagttgttatttttgatatatataaacaacactattatgcccaccatttttttgagatgcatctacacaag *F gaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactccgaacaggtcacaaaaaattaccttaaaaa *F gtcataatattaaattagaataaatatagctgtgagggaaatatatacaaatatattggagcaaataaattgtacataca *F aatatttattactaatttctattgagacgaaatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacg *F acagtcgctccaaggtcgtcgaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaa *F acgtggtgtggtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgtttt *F atgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatccaaagtctact *F tgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattggcacaaatataattacattag *F ctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaaaaaaaacaagcagttatttcggatatatgt *F cggctactccttgcgtcgggcccgaagtcttagagccagatatgcgagcacccggaagctcacgatgagaatggccagac *F ccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtg *F ttcgacgatgtgcagctaatttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaa *F ccatgagaggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaatg *F gtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatccgaaggacgtt *F gacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggcccgcagtccgatcatcggata *F ggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggtaattcggcaatcgttttgcccagaaagtatgtgtcac *F agcgataaagtcgacttcgggcctccctcataaaaactggcagctctgaggtgaacacctaaatcgaatcgattcattag *F aaagttagtaaattattaatatgcaaatgtattctaaacaagacttacatttatcgtggcaaagacgttttgaaaggtca *F tgttggtcaggaagaggaagatggctccgttgatattcatcacgcccacttgcgtgagttgttggcccaaaaagatgagg *F ccaatcaagatggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcatatcttcaacatgttcgcgag *F ttaaatgaaatttatttattttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcag *F gcaattctaactaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccactcac *F cgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgccacaggaccgcccgga *F actgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggcttctccaaatttttggtggccaac *F aactgctccatatcccgggctactttgctaatagcaaaattgtcgcatatcttggcgatccgatcacgggactcgatctc *F ccgtccgggcacaacggccaacacctgtacgtaaaagtccgccggattgtagttggtaggacactgggcacccacgctgg *F ataggagttgagatgtaatgtaatgctagatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttc *F gctgggagtgcccaagaaagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacg *F gctgatgaatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggtaaat *F gagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagtgcctcggaggcgaatgccagacgctt *F cctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgctgacatttgctgagcgaaagctcct *F ggatcacctgatccacgcgggccactcgctgccgataggtcagatgtcgtggcatccgcaccatggcctggaaaatcagg *F tgttccctggccgttagggagccgataaagaggtcatcctgctggacataggcgcacctggcctgcatctccttggcgtc *F cacaggttggccattgagcagtcgcatcccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattca *F gcagggtcgtctttccggcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagttt *F caaattggtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgagcaaa *F tgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctgccgccatccggagcc *F cggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttctcggctagttggccgctccctgaac *F cggagtcctccggcggactgggtggcaggagcgtgccgtagtttttggcctgcccgaagccctggttaatgcagctctgc *F gaagcgtccgctgtcaccctgcaatgataggggatctcaaatatcaactacaagcgttatgctcatctaaccccgaacaa *F aacgaagtatcctacgaagtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgtt *F atattttttgtaaaaaagaatgtagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatcttaaaaattgtct *F tatatagcgagcacagctaccagaataatctgtttcgtgtcactatttgtttgtgcgattgcggtttgggatttttgtgg *F gtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatcaagtgaacttaagatgtatgcaca *F tgtactactcacattgttcagatgctcggcagatgggtgtttgctgcctccgcgaattaatagctcctgatcctcttggc *F ccattgccgggatttttcacactttcccctgcttacccacccaaaaccaatcaccaccccaatcactcaaaaaacaaaca *F aaaataagaagcgagaggagttttggcacagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaa *F accgctgacaaagtgcaactgaaggcggacattgacgctaggtaacgctacaaacggtggcgaaagagatagcggacgca *F gcggcgaaagagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattttgag *F tgagaggtaatcgaaagaacctggtacttcaaatacccttggatcgaagtaaatttaaaactgatcagataagttcaatg *F atgtccagtgcagtaaaaaataaaaaaaaaatatgtttttttaaatctacattctccaaaaaagggttttattaacttac *F atacatactagaattcgagctcgcccggggatcctctagggtacggtaccgctagagtcgaccaattcccggggatcgaa *F agagcctgctaaagcaaaaaagaagtcaccatgtcgtttactttgaccaacaagaacgtgattttcgttgccggtctggg *F aggcattggtctggacaccagcaaggagctgctcaagcgcgatcccgtcgttttacaacgtcgtgagtgggaaaaccctg *F gcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgc *F ccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctg *F gctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatct *F acaccaacgtaacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcaca *F tttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtg *F gtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccg *F gagaaaaccgcctcgcggtgatggtgctgcgttggagtgacggcagttatctggaagatcaggatatgtggcggatgagc *F ggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatga *F tgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctt *F tatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgcc *F gatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggt *F tgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatg *F gtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtc *F atggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatcc *F gaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggca *F tggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgc *F gatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcg *F ctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattattt *F gcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgcta *F cctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactg *F gcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatg *F aaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctg *F gtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgg *F gcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatg *F gtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccg *F cagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggca *F catcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatc *F tgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttca *F cagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacat *F tggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaag *F cagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcagggg *F aaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggc *F gagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggat *F tagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtat *F accccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcgg *F cgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaag *F aaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggac *F cagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataataataaccgggcaggccatgtctgcccgt *F atttcgcgtaaggaaatccattatgtactatttaaaaaacacaaacttttggatgttcggtttattctttttcttttact *F tttttatcatgggagcctacttcccgtttttcccgatttggctacatgacatcaaccatatcagcaaaagtgatacgggt *F attatttttgccgctatttctctgttctcgctattattccaaccgctgtttggtctgctttctgacaaactcggcctcga *F ctctagaggatctttgtgaaggaaccttacttctgtggtgtgacataattggacaaactacctacagagatttaaagctc *F taaggtaaatataaaatttttaagtgtataatgtgttaaactactgattctaattgtttgtgtattttagattccaacct *F atggaactgatgaatgggagcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaagaaatgccatctagt *F gatgatgaggctactgctgactctcaacattctactcctccaaaaaagaagagaaaggtagaagaccccaaggactttcc *F ttcagaattgctaagttttttgagtcatgctgtgtttagtaatagaactcttgcttgctttgctatttacaccacaaagg *F aaaaagctgcactgctatacaagaaaattatggaaaaatattctgtaacctttataagtaggcataacagttataatcat *F aacatactgttttttcttactccacacaggcatagagtgtctgctattaataactatgctcaaaaattgtgtacctttag *F ctttttaatttgtaaaggggttaataaggaatatttgatgtatagtgccttgactagagatcataatcagccataccaca *F tttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgt *F tgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcattttttt *F cactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcgggcgagctcgaattcgtcga *F cctgcagcccaagctttgcgtactcgcaaattattaaaaataaaactttaaaaataatttcgtctaattaatattatgag *F ttaattcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactcaatacgacactcag *F aatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttgccgacgggaccaccttatgttat *F ttcatcatg *F *F *F <<<<<< Attached TEXT file named 'cas-bgal' follows >>>>>> *F catgatgaaataacataaggtggtcccgtcggcaagagacatccacttaacgtatgcttgcaataagtgcgagtgaaagg *F aatagtattctgagtgtcgtattgagtctgagtgagacagcgatatgattgttgattaacccttagcatgtccgtggggt *F ttgaattaactcataatattaattagacgaaattatttttaaagttttatttttaataatttgcgagtacgcaaagcttg *F ggctgcaggtcgactctagaggatccccgggcgagctcgaattcccggggatcccgtcgttttacaacgtcgtgagtggg *F aaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgc *F accgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgcc *F ggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatg *F cgcccatctacaccaacgtaacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttac *F tcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtt *F tcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttt *F tacgcgccggagaaaaccgcctcgcggtgatggtgctgcgttggagtgacggcagttatctggaagatcaggatatgtgg *F cggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcg *F ctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaa *F cagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggt *F ggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcg *F tgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcgga *F ttgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcat *F ggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttc *F gcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaa *F cccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatg *F gtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgc *F gctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccg *F atattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatgg *F ctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgc *F taaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgatta *F aatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatg *F aacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccg *F tttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtgg *F cgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcct *F gaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcaga *F agccgggcacatcagcgcctggcagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgcca *F tcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggc *F tttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctgga *F taacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattacc *F aggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcag *F catcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgt *F tgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaact *F ggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtca *F gacatgtataccccgtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacacca *F gtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgc *F acgcggaagaaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagta *F tcggcggaccagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataataataaccgggcaggccatg *F tctgcccgtatttcgcgtaaggaaatccattatgtactatttaaaaaacacaaacttttggatgttcggtttattctttt *F tcttttacttttttatcatgggagcctacttcccgtttttcccgatttggctacatgacatcaaccatatcagcaaaagt *F gatacgggtattatttttgccgctatttctctgttctcgctattattccaaccgctgtttggtctgctttctgacaaact *F cggcctcgactctagctagaggatctttgtgaaggaaccttacttctgtggtgtgacataattggacaaactacctacag *F agatttaaagctctaaggtaaatataaaatttttaagtgtataatgtgttaaactactgattctaattgtttgtgtattt *F tagattccaacctatggaactgatgaatgggagcagtggtggaatgcctttaatgaggaaaacctgttttgctcagaaga *F aatgccatctagtgatgatgaggctactgctgactctcaacattctactcctccaaaaaagaagagaaaggtagaagacc *F ccaaggactttccttcagaattgctaagttttttgagtcatgctgtgtttagtaatagaactcttgcttgctttgctatt *F tacaccacaaaggaaaaagctgcactgctatacaagaaaattatggaaaaatattctgtaacctttataagtaggcataa *F cagttataatcataacatactgttttttcttactccacacaggcatagagtgtctgctattaataactatgctcaaaaat *F tgtgtacctttagctttttaatttgtaaaggggttaataaggaatatttgatgtatagtgccttgactagagatcataat *F cagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatga *F atgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaat *F aaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcgggcgagc *F tcgaattctagtatgtatgtaagttaataaaacccttttttggagaatgtagatttaaaaaaacatattttttttttatt *F ttttactgcactggacatcattgaacttatctgatcagttttaaatttacttcgatccaagggtatttgaagtaccaggt *F tctttcgattacctctcactcaaaatgacattccactcaaagtcagcgctgtttgcctccttctctgtccacagaaatat *F cgccgtctctttcgccgctgcgtccgctatctctttcgccaccgtttgtagcgttacctagcgtcaatgtccgccttcag *F ttgcactttgtcagcggtttcgtgacgaagctccaagcggtttacgccatcaattaaacacaaagtgctgtgccaaaact *F cctctcgcttcttatttttgtttgttttttgagtgattggggtggtgattggttttgggtgggtaagcaggggaaagtgt *F gaaaaatcccggcaatgggccaagaggatcaggagctattaattcgcggaggcagcaaacacccatctgccgagcatctg *F aacaatgtgagtagtacatgtgcatacatcttaagttcacttgatctataggaactgcgattgcaacatcaaattgtctg *F cggcgtgagaactgcgacccacaaaaatcccaaaccgcaatcgcacaaacaaatagtgacacgaaacagattattctggt *F agctgtgctcgctatataagacaatttttaagatcatatcatgatcaagacatctaaaggcattcattttcgactacatt *F cttttttacaaaaaatataacaaccagatattttaagctgatcctagatgcacaaaaaataaataaaagtataaacctac *F ttcgtaggatacttcgttttgttcggggttagatgagcataacgcttgtagttgatatttgagatcccctatcattgcag *F ggtgacagcggacgcttcgcagagctgcattaaccagggcttcgggcaggccaaaaactacggcacgctcctgccaccca *F gtccgccggaggactccggttcagggagcggccaactagccgagaacctcacctatgcctggcacaatatggacatcttt *F ggggcggtcaatcagccgggctccggatggcggcagctggtcaaccggacacgcggactattctgcaacgagcgacacat *F accggcgcccaggaaacatttgctcaagaacggtgagtttctattcgcagtcggctgatctgtgtgaaatcttaataaag *F ggtccaattaccaatttgaaactcagtttgcggcgtggcctatccgggcgaacttttggccgtgatgggcagttccggtg *F ccggaaagacgaccctgctgaatgcccttgcctttcgatcgccgcagggcatccaagtatcgccatccgggatgcgactg *F ctcaatggccaacctgtggacgccaaggagatgcaggccaggtgcgcctatgtccagcaggatgacctctttatcggctc *F cctaacggccagggaacacctgattttccaggccatggtgcggatgccacgacatctgacctatcggcagcgagtggccc *F gcgtggatcaggtgatccaggagctttcgctcagcaaatgtcagcacacgatcatcggtgtgcccggcagggtgaaaggt *F ctgtccggcggagaaaggaagcgtctggcattcgcctccgaggcactaaccgatccgccgcttctgatctgcgatgagcc *F cacctccggactggactcatttaccgcccacagcgtcgtccaggtgctgaagaagctgtcgcagaagggcaagaccgtca *F tcctgaccattcatcagccgtcttccgagctgtttgagctctttgacaagatccttctgatggccgagggcagggtagct *F ttcttgggcactcccagcgaagccgtcgacttcttttcctagtgagttcgatgtgtttattaagggtatctagcattaca *F ttacatctcaactcctatccagcgtgggtgcccagtgtcctaccaactacaatccggcggacttttacgtacaggtgttg *F gccgttgtgcccggacgggagatcgagtcccgtgatcggatcgccaagatatgcgacaattttgctattagcaaagtagc *F ccgggatatggagcagttgttggccaccaaaaatttggagaagccactggagcagccggagaatgggtacacctacaagg *F ccacctggttcatgcagttccgggcggtcctgtggcgatcctggctgtcggtgctcaaggaaccactcctcgtaaaagtg *F cgacttattcagacaacggtgagtggttccagtggaaacaaatgatataacgcttacaattcttggaaacaaattcgcta *F gattttagttagaattgcctgattccacacccttcttagtttttttcaatgagatgtatagtttatagttttgcagaaaa *F taaataaatttcatttaactcgcgaacatgttgaagatatgaatattaatgagatgcgagtaacattttaatttgcagat *F ggttgccatcttgattggcctcatctttttgggccaacaactcacgcaagtgggcgtgatgaatatcaacggagccatct *F tcctcttcctgaccaacatgacctttcaaaacgtctttgccacgataaatgtaagtcttgtttagaatacatttgcatat *F taataatttactaactttctaatgaatcgattcgatttaggtgttcacctcagagctgccagtttttatgagggaggccc *F gaagtcgactttatcgctgtgacacatactttctgggcaaaacgattgccgaattaccgctttttctcacagtgccactg *F gtcttcacggcgattgcctatccgatgatcggactgcgggccggagtgctgcacttcttcaactgcctggcgctggtcac *F tctggtggccaatgtgtcaacgtccttcggatatctaatatcctgcgccagctcctcgacctcgatggcgctgtctgtgg *F gtccgccggttatcataccattcctgctctttggcggcttcttcttgaactcgggctcggtgccagtatacctcaaatgg *F ttgtcgtacctctcatggttccgttacgccaacgagggtctgctgattaaccaatgggcggacgtggagccgggcgaaat *F tagctgcacatcgtcgaacaccacgtgccccagttcgggcaaggtcatcctggagacgcttaacttctccgccgccgatc *F tgccgctggactacgtgggtctggccattctcatcgtgagcttccgggtgctcgcatatctggctctaagacttcgggcc *F cgacgcaaggagtagccgacatatatccgaaataactgcttgtttttttttttaccattattaccatcgtgtttactgtt *F tattgccccctcaaaaagctaatgtaattatatttgtgccaataaaaacaagatatgacctatagaatacaagtatttcc *F ccttcgaacatccccacaagtagactttggatttgtcttctaaccaaaagacttacacacctgcataccttacatcaaaa *F actcgtttatcgctacataaaacaccgggatatattttttatatacatacttttcaaatcgcgcgccctcttcataattc *F acctccaccacaccacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcaccttttgttcga *F cgaccttggagcgactgtcgttagttccgcgcgattcggttcgctcaaatggttccgagtggttcatttcgtctcaatag *F aaattagtaataaatatttgtatgtacaatttatttgctccaatatatttgtatatatttccctcacagctatatttatt *F ctaatttaatattatgactttttaaggtaattttttgtgacctgttcggagtgattagcgttacaatttgaactgaaagt *F gacatccagtgtttgttccttgtgtagatgcatctcaaaaaaatggtgggcataatagtgttgtttatatatatcaaaaa *F taacaactataataataagaatacatttaatttagaaaatgcttggatttcactggaactagaattaattcggctgctgc *F tctaaacgacgcatttcgtactccaaagtacgaattttttccctcaagctcttattttcattaaacaatgaacaggacct *F aacgcacagtcacgttattgtttacataaatgattttttttactattcaaacttactctgtttgtgtactcccactggta *F tagccttcttttatcttttctggttcaggctctatcactttactaggtacggcatctgcgttgagtcgcctccttttaaa *F tgtctgaccttttgcaggtgcagccttccactgcgaatcattaaagtgggtatcacaaatttgggagttttcaccaaggc *F tgcacccaaggctctgctcccacaattttctcttaatagcacacttcggcacgtgaattaattttactccagtcacagct *F ttgcagcaaaatttgcaatatttcatttttttttattccacgtaagggttaatgttttcaaaaaaaaattcgtccgcaca *F caacctttcctctcaacaagcaaacgtgcactgaatttaagtgtatacttcggtaagcttcggctatcgacgggaccacc *F ttatgttatttcatcatg *F *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From crosby Sun Aug 4 18:43:01 1996 *F Date: Sun, 4 Aug 96 18:42:59 EDT *F From: crosby (Madeline Crosby) *F To: cthummel@qmserver.genetics.utah.edu *F Subject: Re: Casper-bgal sequences *F Cc: crosby *F Content-Length: 1134 *F *F *F Carl, *F *F Thanks! (Sorry to be a bit slow to reply -- I've been on vacation.) *F *F These corrections should be incorporated into the FlyBase-compiled *F sequences within the next week or two. *F *F Two questions: *F *F 1) In pCaSpeR-AUG-Bgal your corrected junction between SV40 and CaSpeR *F includes an EcoRI site. If this is correct, EcoRI can not *F be used as a cloning site. Are there, indeed, two EcoRI sites?? *F *F 2) Is the junction between lacZ and SV40 in pCaSpeR-Bgal identical to *F that in CaSpeR-hs43-lacZ? *F *F *F For the GenBank submissions I think I would prefer not to use the *F standard FlyBase format (P transposons always presented P5' to P3' and *F vectors broken within the polylinker). I am inclined to prepare them in *F the orientation you have your sequences (with the lacZ 5' to 3'), *F starting with the P sequences and appending the vector sequences. So, *F unless I hear any objections.... *F *F Who should be included in the Thummel lab citation? I assume we *F should cite the Thummel et al. 1988 Gene paper, and for CaSpeR-Bgal, *F the DIN/DIS correction. Any others? *F *F *F I'm really pleased that we were able to pull this together! *F *F --Lynn *F *F *F ----- End Included Message ----- *F *F *F *F ----- Begin Included Message ----- *F *F Date: 5 Aug 1996 11:18:51 U *F From: 'CThummel' *F Subject: Re: Casper-bgal sequences *F To: 'Madeline Crosby' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 3289 *F *F RE>>Casper-bgal sequences 8/5/96 *F *F Thanks Lynn - I am glad to hear that you take vacations! I would imagine that *F staring at sequences gets to be a little tedious after awhile! Sorry about *F the error in the SV40 CaSpeR junction of pCaSpeR-AUG-Bgal. I was just *F focusing on the junction sequences. There is a base change in the CaSpeR *F sequence, immediately downstream from the junction that inactivates the EcoRI *F site. The sequence should read: ...GAATTGGTCGACC... Unfortunately, I found a *F few other single base changes further downstream in CaSpeR, but I do not want *F to get involved in proofing all 7.8 kb!! What a pain. *F *F The lacZ/SV40 junctions are different between CaSpeR-Bgal and *F CaSpeR-hs43-lacZ. Vince Pirrotta independently constructed the latter vector, *F using pCaSpeR4. That accounts for the 6 bp difference between the two *F junction sequences. *F *F I agree with you, that it would be best to have the vector sequences present *F in the GenBank submission, and have the lacZ sequences ordered 5' to 3'. For *F citations, I think that the Gene paper is sufficient (and DIN/DIS correction *F for pCaSpeR-Bgal). Thanks for all your help! I am so happy that this got *F done - I continue to get requests for these sequences. Please let me know *F when you have accession numbers, *F Carl *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From crosby Wed Aug 7 10:56:31 1996 *F Date: Wed, 7 Aug 96 10:56:29 EDT *F From: crosby (Madeline Crosby) *F To: cthummel@qmserver.genetics.utah.edu *F Subject: Casper-AUG-bgal sequences *F Cc: crosby *F Content-Length: 515 *F *F *F Carl, *F *F Sorry to be harrassing you, again, but this is a little disturbing: *F *F > Unfortunately, I found a *F >few other single base changes further downstream in CaSpeR, but I do not want *F >to get involved in proofing all 7.8 kb!! *F *F There could easily be errors within the polylinker sequence, which is *F only another 20-25bp. (Many necessary fill-ins, etc., performed during *F constructions never get mentioned.) These I would like to know about. *F The sequence beyond that should be okay (P3' published sequence). *F *F --Lynn *F *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From cthummel@qmserver.genetics.utah.edu Wed Aug 7 12:10:35 1996 *F Date: 7 Aug 1996 10:10:20 U *F From: 'CThummel' *F Subject: Re: Casper-AUG-bgal sequence *F To: 'Madeline # *U FBrf0087820 *a Thummel *b C.S. *t 1996.8.8 *T personal communication to FlyBase *u *F 08 Aug 1996 *F *F Personal communication from Carl Thummel: data curated from Web page *F maintained by Carl Thummel entitled 'P-element vector maps' at *F http://www-hhmi.genetics.utah.edu/thummel/pelement.html. Data *F downloaded and archived as hard copy 08 Aug 1996. *F ____________________________________________________________________ *F *F From crosby Wed Aug 7 14:53:39 1996 *F Date: Wed, 7 Aug 96 14:53:30 EDT *F From: crosby (Madeline Crosby) *F To: cthummel@qmserver.genetics.utah.edu *F Subject: citing Web page *F Cc: crosby *F Content-Length: 645 *F *F *F Carl, *F *F On a different subject: *F *F I would like to incorporate information from your P-element vector *F maps page into the relevant FlyBase reports. FlyBase is *F excruciatingly meticulous about atttributing all data we report. *F We are somewhat at a loss as to how to cite Web pages, given their *F dynamic, and often transient, nature. Our short-term fix is to *F download what we need, note the date, and archive a hard copy, which *F we treat as a personal communication. I just need to get confirmation *F from you that you do not object to having information from the current *F P-element vectors page cited as fact and attributed to you. :-) !! *F *F --Lynn *F *F ______________________________________________________________________ *F From cthummel@qmserver.genetics.utah.edu Wed Aug 7 15:24:10 1996 *F Date: 7 Aug 1996 13:26:04 U *F From: 'CThummel' *F Subject: Re: citing Web page *F To: 'Madeline Crosby' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 2278 *F *F RE>citing Web page 8/7/96 *F *F No problem Lynn. You should feel free to copy our P element Web page. I *F would like to disseminate this information more widely, and so FlyBase would *F only be a help. My main goal in getting this page set up was so that we could *F avoid mailing maps with each DNA sample. We now send DNA as spots on paper, *F and tell the recipient to access the Web page. We are, however, in the *F process of updating our page. The current format requires the user to *F download each map onto their hard disk, and then open it with a drawing *F program. We are going to modify this so that they can see and print the maps *F directly from their Web browser. You may want to wait until we have these *F modified files available (within the next week or so) *F Carl *F *F -------------------------------------- # *U FBrf0089302 *a Todo *b T. *t 1996.4.30 *T personal communication to FlyBase *u *F Archived. # *U FBrf0089303 *a Anderson *b K. *t 1996.6.13 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Jun 13 15:42:20 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 13 Jun 96 15:42:15 BST *F To: kathryn_anderson@maillink.berkeley.edu *F Subject: Help FlyBase - ea5022rx1 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 916 *F Dear Dr. Anderson, *F I am a curator of FlyBase working at the Cambridge, England site with Prof. *F M. Ashburner. *F During curation I have come across ea5022rx1. In papers this is used as *F a ea- mutation (in combination with ea4). And in: *F FBrf0083893 == Erdelyi et al., 1995, Nature 377(6549): 524--527 *F is stated to fail to complement TmII mutations. *F With this information I would like to make Df(3R)ea5022rx1. Currently *F Df(3R)ea5022rx1 is a synonym under eaD10 (L&Zs symbol for 5022). *F Could you please tell me if ea5022rx1 is broken in ea or completely *F deletes ea? *F How is this mutation related to ea5022 (a point mutation) is it *F a revertant? *F I have traced a lot of this information via papers from R. Chasan and *F references to her PhD thesis, but unfortunately I only have a snail mail *F address for her. If you would like to pass this query on to her then *F please do so. *F Many thanks, *F Eleanor Whitfield *F From anderson@mendel.berkeley.edu Thu Jun 13 17:31:44 1996 *F Date: Thu, 13 Jun 1996 09:22:11 -0700 (PDT) *F X-Mailer: Eudora Pro 2.1.3 *F To: Eleanor Whitfield (Genetics) *F From: anderson@mendel.berkeley.edu (Kathryn Anderson) *F Subject: Re: Help FlyBase - ea5022rx1 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-Length: 1778 *F Dear Dr. Whitfield, *F ea5022RX1 is an X-ray induced revertant of ea5022 made by J. Szabad. *F ea5022RX1 completely deletes easter; it also appears to delete all the DNA *F in the clone lambaTMR16 from the Eric Fyrberg walk through the region, but *F we have not mapped its breakpoints. *F I hope this is helpful. *F Sincerely, *F Kathryn Anderson # *U FBrf0089304 *a Miklos *b G. *t 1996.5.22 *T personal communication to FlyBase *u *F Fax message: *F Dear Dr. Miklos, *F I am a curator of FlyBase working at the Cambridge, England, site with *F Prof. M. Ashburner. *F I am curating your paper: *F FBrf0085507 == Maleszka et al., 1996, Proc. natn. Acad. Sci. USA. 93(1): *F 447--451 *F in which you discuss a gene tweety. The three letter symbol you have *F assigned to tweety, twe, has unfortunately already been used for twine. *F Therefore we cannot use twe for tweety. Do you have another three letter *F symbol that can be used? *F Apologies for having to bring this to your attention, *F Eleanor Whitfield *F FlyBase *F From miklos@nsi.edu Fri Jun 14 23:44:13 1996 *F Date: Fri, 14 Jun 1996 15:36:12 -0700 (PDT) *F X-Sender: glgm@popmail *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: eleanor@gen.cam.ac.uk *F From: miklos@nsi.edu (George L. Gabor Miklos) *F Subject: tweety *F Content-Length: 331 *F dear eleanor, sorry about the snafu with the symbols for tweety. could we *F call it twt ? yours sincerely george miklos *F From eleanor@gen.cam.ac.uk Mon Jun 17 08:16:07 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 17 Jun 96 08:16:02 BST *F To: miklos@nsi.edu *F Subject: Re: tweety *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 344 *F Hi, *F >could we call it twt ? *F Unfortunately not, twt is the symbol for twirled tips. *F Sorry about this, do you have another choice of symbol? *F When you have decided upon a gene symbol I shall change the genes file *F accordingly, then there will be no concerns about losing your symbol *F again. *F Thank you for your patience, *F Eleanor Whitfield *F FlyBase *F From miklos@nsi.edu Sat Jun 22 17:17:06 1996 *F Date: Sat, 22 Jun 1996 09:08:55 -0700 (PDT) *F X-Sender: glgm@popmail *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: miklos@nsi.edu (George L. Gabor Miklos) *F Subject: Re: tweety *F Content-Length: 430 *F Dear Eleanor, i am sorry for causing you so much grief. What about tty *F ?if not twy ? maybe you should have the honor of naming it, since you *F are in the best position to do so warmest regards george miklos *F From eleanor@gen.cam.ac.uk Mon Jun 24 07:58:38 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 24 Jun 96 07:56:07 BST *F To: miklos@nsi.edu *F Subject: Re: tweety *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 393 *F Hi, *F >i am sorry for causing you so much grief *F It is not a problem to me, I am concerned that you are having to change *F your gene symbol. *F >What about tty *F This was your first choice and it is available - so tty it is. *F The genes file shall be updated to reflect this change, these updates will *F be publically available in the next genes release. *F Many thanks for all your help, *F Eleanor Whitfield # *U FBrf0089305 *a Baker *b N. *t 1996.8.23 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Fri Aug 23 12:23:37 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 23 Aug 96 12:23:35 BST *F To: baker@aecom.yu.edu *F Subject: Help FlyBase - wgCX3 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1499 *F Hi Nicholas, *F I am a curator for FlyBase working at the Cambridge, England, site with *F Prof M. Ashburner. While curating a paper I came across wgCX3, of course *F now known as wgl-16. I went to all the references we have listed for *F wgCX3 and it soon became apparent that this chromosome is a little *F confusing. I was hoping you could help me to clear this up. *F \*x FBrf0046100 == Baker, 1987, EMBO J. 6(6): 1765--1774 *F reports a breakpoint between +10.8 and +15.0 of the wg molecular map, this *F is a complex rearrangement and not a deficiency. *F \*x FBrf0047884 == Baker, 1988, Development 102: 489--497 *F reports a breakpoint downstream of the transcription unit that brings other *F sequences close to the 3' terminus of @wg@. Deletion of other genes other *F than wg. *F \*x FBrf0066940 == van den Heuvel et al., 1993, EMBO J. 12: 5293--5302 *F reports a 17kb insertion of unknown DNA between 2-4kb from the *F transcriptional endpoint of wg. *F Could you please clarify the situation? *F Many thanks *F Eleanor Whitfield *F FlyBase *F From baker@aecom.yu.edu Fri Aug 23 15:32:57 1996 *F Date: Fri, 23 Aug 1996 10:23:58 -0400 (EDT) *F X-Sender: baker@mailserver.aecom.yu.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: baker@aecom.yu.edu (Dr. Nicholas Baker) *F Subject: Re: Help FlyBase - wgCX3 *F Content-Length: 2091 *F Dear Eleanor, *F Sorry that wgCX3 is so confusing. The allele was X-ray induced, *F and based on orcein squashes appeared to involve a deficiency ~28A-C. *F Homozygous embryos died before secreting cuticle, consistent with deletion *F of many genes. In trans to null alleles of wg, wgCX3 gave pupal lethality, *F indicating that it is not a null allele of wg, which cannot have been *F deleted in wgCX3. Southern blotting showed that most of the wg gene was *F unaltered but there was a breakpoint in the +14.6 - +16.3 interval. To *F test whether this was one end of a deficiency, blots were performed with *F wgCX3/wg1 DNA, since wg1 deletes some DNA right of +16.3, but this DNA was *F still present in the wgCX3 chromosome. From this I concluded that the 3' *F breakpoint in wgCX3 was not one end of a deficiency. The relationship of *F the wgCX3 lesion to the cytology of the chromosome was not clear; they *F might be related parts of a complex rearrangement, or might be independent *F events in the same chromosomal region. *F I understand the van den Heuvel et al to have shown a 17kb *F insertion into wgCX3. I assume this is the event in the +14.6-+16.3 *F interval that I identified in Southern blots. As I recall, their paper *F gives no further insight as to the cytological appearance of wgCX3, or to *F the apparent mutation of other genes required to account for the arrest of *F wgCX3 homozygotes prior to embryonic cuticle secretion. Marcel van den *F Heuvel (now with Phil Ingham??) might have further unpublished data, but *F unless he does so you can draw no inference of any deletion associated with *F the insertion, or anywhere else in the cloned wg region, and the reason for *F the altered cytology is unclear and very possibly unrelated to wg. *F hope this helps, *F regards *F Nick Baker *F From eleanor@gen.cam.ac.uk Fri Aug 23 16:06:10 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 23 Aug 96 16:05:50 BST *F To: baker@aecom.yu.edu *F Subject: Re: Help FlyBase - wgCX3 *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 952 *F Hi Nick, *F Thank you very much for your prompt reply, it is always appreciated. *F I am now clear about the complete picture of the chromosome rearrangements *F given in your papers, thank you. If you have no objections I would like to *F curate your correspondance as a personal communication to FlyBase. *F Many thanks for your help, *F Eleanor Whitfield *F From baker@aecom.yu.edu Fri Aug 23 16:55:40 1996 *F Date: Fri, 23 Aug 1996 11:46:39 -0400 (EDT) *F X-Sender: baker@mailserver.aecom.yu.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: baker@aecom.yu.edu (Dr. Nicholas Baker) *F Subject: Re: Help FlyBase - wgCX3 *F Content-Length: 2024 *F Dear Eleanor, *F Having checked in my thesis I see that I said wgCX3 was a *F deficiency in 28A. There is a photo there and it looks like it might go *F into 28B but I don't want to be quoted on this based on memory from a *F decade ago. Please can you leave it as 'small deficiency in 28A region' - *F unless anyone wants to do a careful cytological analysis. *F By all means include my message in fly base. Everything in it is *F in my thesis 'wingless: a gene required for segmentation in Drosophila', *F Univ. of Cambridge 1986, and this may be more appropriate than pers. comm. *F However, the details could also be summarised as: 'the wgCX3 *F mutation is associated with an insertion of unknown DNA just 3' to the *F transcription unit (Baker 1987; van den Heuvel 1993). In addition, the *F cytological appearance of a deletion in 28A and the early demise of wgCX3 *F homozygous embryos prior to cuticle secretion imply deletion or alteration *F of other genes, but the molecular basis has not been determined (Baker *F 1988).' *F regards *F Nick Baker # *U FBrf0089306 *a Banga *b S. *t 1996.5.2 *T personal communication to FlyBase *u *F From: Satnam Banga *F Subject: nomenclature of mutations *F To: rd120@gen.cam.ac.uk *F Date: Thu, 2 May 1996 11:46:33 -0400 (EDT) *F Ref: \*x FBrf0079877==Banga etal., 1995, Mol Gen. Genet. 246:148-155 *F Dear Dr. Drysdale: *F Sorry for replying late. The old designations in my manuscript are as follows: *F Old designation New Designation *F l(1)9-21 = l(1)14Ca *F l(1)9-38-1 = l(1)14Cc *F l(1)19-134 = l(1)14Cd *F l(1)k17a = l(1)14Cb *F l(1)14-12 = l(1)14Ce *F l(1)19-153 = l(1)14Cf *F I hope this will solve the confusion. If you have any questions, please *F let me know. *F With regards, *F Satnam Banga, PhD. # *U FBrf0089307 *a Kassis *b J. *t 1996.5.30 *T personal communication to FlyBase *u *F From: Rachel Drysdale (Genetics) *F Date: Wed, 29 May 96 14:42:43 BST *F To: kassis@helix.nih.gov *F Hi Judith, *F I have a question about an insertion line that you generated. It has been *F referred to in the literature as 17-en-52, an insertion of a P element that *F contains 'lacZ' into exon 1 of en. The paper I am curating is: *F \*x FBrf0084183 == Mellerick and Nirenberg, 1995, Dev. Biol. 171(2): 306--316 *F Have you published this insertion anywhere? I couldn't find it in your *F PNAS, Genes Dev or either of the Genetics papers. If not, please could you *F tell me which P element inserted into en to cause this allele? That way I *F can get the details more correct than vague... *F with best wishes, *F Rachel *F Ref: rd2353.h *F From kassis@helix.nih.gov Thu May 30 14:03:28 1996 *F Date: Thu, 30 May 1996 08:59:30 -0400 *F To: Rachel Drysdale (Genetics) *F Rachel: *F 17en52 was one of the insertions of Pen1(rosy) generated in our *F PNAS paper. We list 7 insertions into 48A in Table 1, but don't name the *F lines. *F Best wishes, *F Judy # *U FBrf0089308 *a Shearn *b A. *t 1996.6.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Jun 25 14:29:55 1996 *F From: Rachel Drysdale (Genetics) *F Date: Tue, 25 Jun 96 14:29:49 BST *F To: bio_cals@jhuvms.hcf.jhu.edu *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 1238 *F Hi Allen, *F I have a question about a marked balancer that you published in 1993. It *F is the y+ CyO chromosome published in Timmons et al., 1993, Dev. Biol. *F 158(2): 364--379. *F It was said to have been constructed by Elizabeth Woodhouse (Materials and *F Methods, bottom of page 365). *F Has this element been previously published? If so, can you tell me which *F y+ construct was used? We have records for many y+ constructs in *F FlyBase so its possible that we already have an entry for it, and I would *F like to link this balancer to the correct construct, if at all possible. *F If it is one that Elizabeth made herself, if you could let me know a couple *F of details, such as size of yellow genomic DNA fragment, and transformation *F vector, then that would be wonderful. *F many thanks for your help, *F with best wishes, *F Rachel. *F From bio_cals@jhu.edu Tue Jun 25 15:13:35 1996 *F Date: Tue, 25 Jun 1996 10:02:04 -0400 (EDT) *F Date-Warning: Date header was inserted by jhmail.hcf.jhu.edu *F From: bio_cals@jhu.edu (Allen Shearn) *F Subject: Re: helping FlyBase *F To: Rachel Drysdale (Genetics) *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-Transfer-Encoding: 7BIT *F Content-Length: 665 *F Dear Rachel, *F The y+ construct in a P-element vector was made by Pam Geyer at The *F University of Iowa. The insert we started with was on an otherwise unmarked *F 2nd chromosome. I am afraid that I do not know the details of the *F construct. We have subsequently transposed the same insert onto a TM3 *F chromosome that is marked with Ser and e but not Sb. The y+ has been *F completely stable on both balancers. *F Sincerely, *F Allen *F ----------------------------------------------------------------- *F ALLEN SHEARN *F Department of Biology Johns Hopkins University Baltimore, MD 21218 *F PHONE 410-516-7285 FAX 410-516-5213 # *U FBrf0089309 *a Drysdale *b R. *t 1996.6.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Jun 25 20:29:09 1996 *F From: Rachel Drysdale (Genetics) *F Date: Tue, 25 Jun 96 20:29:09 BST *F To: rd120@gen.cam.ac.uk *F Subject: mbc *F Content-Length: 218 *F Embryos homozygous for the mbcC1 chromosome show a variably expressed *F 'dorsal open' phenotype. Homozygous Df(3R)mbc-R1 embryos show a similar *F though typically more extreme dorsal open phenotype, as do *F mbcC1/Df(3R)mbc-R1 hemizygotes. # *U FBrf0089310 *a Doherty *b D. *t 1996.7.26 *T personal communication to FlyBase *u *F From dadoher@itsa.ucsf.edu Fri Jul 26 18:04:55 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 26 Jul 1996 09:57:18 -0700 *F To: Rachel Drysdale (Genetics) *F From: dadoher@itsa.ucsf.edu (Dan Doherty) *F Subject: Re: \*x FBrf0086385 == Doherty et al., 1996, Genes Dev. 10(4): 421--434 *F Content-Length: 468 *F Rachel, *F Here is the information you requested about the UAS nuclear lacZ *F construct. Let me know if you have any other questions. *F Take care, *F Dan *F >origin of nuclacZ: *F >vector D237 = Act5c>Draf+>nuc-lacZ from Gary Struhl (Struhl and Basler, *F >1992 Cell >72:527-540 where he describes the FLP-out technique) *F >transformation vec: pUAST white+ in flies and amp resistant in bacteria *F >how many Gal4 binding sites:5 *F >what promoter: hsp70. *F >published before: no # *U FBrf0089311 *a Noll *b M. *t 1996.8.17 *T personal communication to FlyBase *u *F >From noll@molbio2.unizh.ch Sat Aug 17 11:20:15 1996 *F X-Sender: noll@rzu-mailhost.unizh.ch *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Sat, 17 Aug 1996 12:11:59 +0100 *F To: Aubrey de Grey *F From: noll@molbio2.unizh.ch (Markus Noll) *F Subject: Re: DAPA/P110 *F Content-Length: 2409 *F Dear Aubrey, *F The gene that we have called DAPA 10 years ago (!) does *F not encode a protein mediating adhesion as we had thought initially. *F We have never published anything on it and have dropped the project *F long ago. Its transcript is located between 0 and - 10 kb on our *F published map (Justice et al., Genes & Dev. 9, 534-546, 1995). *F Homozygous deficiencies of the gene are fertile and viable and show *F no obvious phenotype. Therefore, I would suggest that the tentative *F name DAPA for this gene be eliminated. The product of the gene *F is a 110 kDa protein, which is homologous to prosaposin from vertebrates *F (rat, mouse, man). Therefore, a better name for the gene would be *F 'sap-r' for 'saposin-related', as we have called it later when the *F first vertebrate homologues were found. *F I hope this answers your questions. I am also sending this *F message to Peter Bryant to make sure that he will also refer to it *F in the future as sap-r. *F With best regards, *F Markus # *U FBrf0089312 *a Coates *b D. *t 1996.8.21 *T personal communication to FlyBase *u *F >From ag24@gen.cam.ac.uk Tue Aug 20 14:59:37 1996 *F From: Aubrey de Grey *F Date: Tue, 20 Aug 96 14:59:30 BST *F To: d.coates@leeds.ac.uk *F Subject: Ance etcetc *F Cc: ag24@gen.cam.ac.uk *F Content-Length: 1737 *F Dear David, *F FlyBase is just catching up on the story of this gene, and (as you are *F no doubt all too aware!) there is some work to do in sorting out a *F complete and consistent story from the literature. In FlyBase at the *F moment we have three separate genes: Ance, Race and l(2)34Eb. We have *F looked at all the references we know of, namely the GenBank records *F U25344 and U34599, the JBC paper from your group, the Mech. Dev. paper *F from Mike Levine's group, and your respective abstracts from the Atlanta *F fly meeting and the 1994 neurobiology meeting. It's not enough! *F Our understanding so far is as follows: *F \- Levine's group established the identity of 'Race' and l(2)34Eb *F \- They did not establish that 'Race' had the predicted proteolytic *F activity; hence the 'R'. *F \- You did establish that activity. *F \- They mapped their gene to 34E, you mapped yours to 34A. *F \- The sequences differ by just enough to make us nervous -- about ten *F differences, counting a 10bp-long frame shift as one. *F The question of priority is a very close call -- it probably depends *F on whether we count abstracts as publications, something where we have *F never had a very clear policy -- but we are strongly in favour of using *F Ance on the basis that ACE activity has been shown. However we would *F rather like to be sure that there are not really two genes -- and even *F that is not counting 'Acer' (GenBank X96913) from Alan Shirras! *F So .... is there any more recent evidence (published or not) that shows *F whether your and Levine's genes are the same? 34E is in the Ashburner *F deficiency universe and 34A is not, so we are pretty sure that if it is *F only one gene then it must be in 34E given Levine's data. Have you and *F they resolved the sequence conflicts? *F \----- End Included Message ----- *F \----- Begin Included Message ----- *F >From pab6dc@gps.leeds.ac.uk Wed Aug 21 12:48:58 1996 *F Comments: Authenticated sender is *F From: 'David Coates' *F To: Aubrey de Grey *F Date: Wed, 21 Aug 1996 12:38:16 +0000 *F Subject: Re: Ance etcetc *F Priority: normal *F X-Mailer: Pegasus Mail for Win32 (v2.42a) *F Content-Length: 1788 *F Dear Aubrey, *F The Ance/Race/l(2)43Eb story..... *F 1. We believe that all three are the same: one of the sequencing *F differences has been resolved - we had the frameshift, shown on *F resequencing to be an error. The other erros are clustered and look *F like sequencing errors - Tracy Williams in France resequenced much of *F Ance while doing mutagenesis experiments, so we are confident our cDNA *F is properly entered - the frameshift error has been corrected in the *F GenBank entry. The other differences may be varietal - the protein *F region affected at the N-terminus is not in a conserved part of the *F gene, but as far as I know the Levine clone has not been rechecked The *F C to S change may be important, or may be an error -at this stage we *F don't know. *F 2. Our in situ data is much less reliable than Levine's - *F we think the 34E location is right, not 34A *F 3. Acer is clearly different - maps to a different location, and is *F very different at the sequence level - we are currently expressing it to see *F whether is also is a peptidyl dipeptidase (1). *F 4. First _published_ reference to AnCE is *F is in Biochem Soc Transactions (2). *F 1. Taylor, C.A.M., Coates, D. & Shirras, A.D. Gene In press (1996). *F 2. Cornell, M.J., Coates, D. & Isaac, R.E. Biochemical Society *F Transactions 21, 243S (1993). *F Hope this clears things up - I'm off to France for 12 months, but this *F email address will still get me.. *F DavidC *F \********************************************************** *F David Coates *F Department of Biology *F The University of Leeds *F Leeds LS2 9JT *F UK *F Ph: +44 113 233 2891 e-mail: PAB6DC@BMB.LEEDS.AC.UK *F Fax:+44 113 233 2882 *F \********************************************************** # *U FBrf0089313 *a Coyne *b J.A. *t 1996.8.22 *T personal communication to FlyBase *u *F From jcoyne@pondside.uchicago.edu Thu Aug 22 15:08:47 1996 *F Date: Thu, 22 Aug 1996 09:01:44 -0600 *F To: flybase-updates@morgan.harvard.edu *F From: jcoyne@pondside.uchicago.edu (Jerry Coyne) *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 608 *F New mutation in Drosophila simulans. *F I have found a new mutation in Drosophila simulans which is identical in *F phenotype and gene (as determined by crossing) to the glass mutation of *F Drosophila melanogaster. I call this glass^1 (gl^1) and have sent a copy *F to the Species Center at Bowling Green. Please add this information to *F FlyBase. *F Thank you. *F Jerry Coyne *F __________________________________ *F Jerry Coyne *F Department of Ecology & Evolution *F The University of Chicago *F 1101 E. 57th Street *F Chicago, IL 60637 USA *F Phone: (312) 702-1105 or 702-1106 *F Fax: (312) 702-9740 *F email: jcoyne@pondside.uchicago.edu # *U FBrf0089314 *a Goriely *b A. *t 1996.8.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Aug 22 14:12:13 1996 *F From: Rachel Drysdale (Genetics) *F Date: Thu, 22 Aug 96 14:12:11 BST *F To: desplan@rockvax.rockefeller.edu *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F Content-Length: 1978 *F Dear Claude, *F I am writing about Anne's paper *F \*x FBrf0087230 == Goriely et al., 1996, Development 122(5): 1641--1650 *F which I am curating for FlyBase. *F You mention no cytological location for your D-gsc, but we know from Anne *F that 21C5-21C6 is the correct location of D-gsc (or Gsc, as it was named *F from its sequence record by FlyBase). *F FlyBase has gene entries for 60Mun1 (and 60Mun2) which record (correctly) *F their cytological location as being 60C (Dessain and McGinnis, 1993, Adv. *F Dev. Biochem. 2: 1--55). *F Since 60Mun1 = Gsc, the implication is that the original reported cytology *F for 60Mun1 was incorrect due to someone mistaking one end of chromosome 2 *F for the other. Could you (or Scott) confirm that my interpretation is *F correct? Does this also mean that 60Mun2 should have a cytological *F location of 21C-21D? The 60Mun1 gene record will be merged with the Gsc *F record as a result of your paper, but 60Mun2 will stay separate and I *F would like to be sure that FlyBase is reporting its cytological location *F accurately. *F . *F . *F . *F Many thanks for your help, *F with best wishes, *F Rachel. *F \-------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From anne.goriely@human-anatomy.oxford.ac.uk Thu Aug 22 17:02:13 1996 *F Date: Thu, 22 Aug 1996 16:53:05 +0100 (BST) *F From: Anne Goriely *F To: Rachel Drysdale *F Subject: Re: helping FlyBase *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 4191 *F Dear Rachel, *F I received your message through Claude who asked me to answer *F your queries. *F Your interpretation of the cytological location of the Mun genes is *F correct. It was a mistake made in the 1st report by Dessain & McGinnis. *F Both genes map very close to each other, actually on a common genomic *F phage, at approximately 10kB of each other. They are both located at 21 *F C5-6. This is also what Mounou Hahn and Herbert Jackle found and their *F paper should be coming soon or is already out in EMBO J. (they only *F worked on Gsc though). Claude and me are currently writing a short report *F about the other Mun gene, 60Mun2 that we simply call Munster and which is *F specifically expressed in Bolwig organs. *F I hope these few lines will answer your questions. *F Do not hesitate to get in touch with me again if necessary. *F Yours Sincerely *F Anne Goriely # *U FBrf0089315 *a Kress *b H. *t 1996.7.12 *T personal communication to FlyBase *u *F Also archived. *F The following information was received from Horst Kress on disc and *F in hard copy on 12 July 1996. The data were compiled by Kress on the request of *F FlyBase to revise the D. virilis gene records. This file is the *F reference to all original observations of H. Kress, and (except for *F minor reformating from the original PC files) is presented as received. *F There were minor corrections on the hard copy, which is available from FlyBase. *F ============================================================== *F updates.txt *F additions for genes already existing in the gene catalogue *F *F \*a Dvir\ac *F \*z FBgn0013102 *F \*e acute *F \*b 6-0.0 *F \*M ac *F \*p pointed wings *F \# *F \*a Dvir\bb *F \*z FBgn0013103 *F \*e bobbed *F \*b 1-170.5 *F \*M bb *F \*x Chino (unpublished observation) *F \*p in females bristles small, sclerites scaly *F \# *F \*a Dvir\br *F \*z FBgn0013106 *F \*e broad *F \*b 1-96.0 *F \*x Kikkawa (unpublished observation) *F \*p wings about 4/5 normal length, slightly broader; crossveins closer together, wing margins \*p scalloped *F \*M br *F \# *F \*a Dvir\exu *F \*z FBgn 0013077 *F \*c 50 C6,7 (Kress, unpublished observation) *F \# *F \*a Dvir\hb *F \*z FBgn0013116 *F \*e hunchback *F \*c 21C *F \*x FBrf0057925 == Lozovskaya et al., 1993, Chromosoma 102, 253-266 *F \*M hb *F \# *F Dvir\mam *F \*a \*z FBgn0013119 *F \*e mastermind *F \*c 59D *F \*x FBrf0057925 == Lozovskaya et al., 1993, Chromosoma 102, 253-266 *F \*M mam *F \# *F \*a Dvir\ro *F \*z FBgn 013136 *F \*e rough *F \*b 1-74.9 *F \*x Moses (unpublished observations) *F \*p small, rough eye; small marginal hairs on wings; female sterile *F \*M ro *F \# *F \*a Dvir\sc *F \*z FBgn0013139 *F \*e scute *F \*b 1-3.8 *F \*M sc *F \*x Metz (unpublished observations) *F \*p scutellar bristles are missing *F \# *F \*a Dvir\si *F \*z FBgn *F \*e singed *F \*b 1-50.0 *F \*M singed *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*c 7C *F \*x FBrf0057925 == Lozovskaya et al., 1993. Chromosoma 102, 253-266 *F \*p all bristles and hairs twisted and gnarled *F \# *F \*a Dvir\su(s) *F \*c 4D *F \*x FBrf0057925 == Lozovskaya et al., 1993, Chromosoma 102, 253-266 *F \# *F \*a Dvir\Lgp-1 *F \*b 1-144.4 *F \*q Recombination frequency between @ap@ and the proximally localized larval glue protein gene @Lgp-1@ is 3% (FBrf 0045117 == Kress, 1986, Naturwissenschaften 73: 180--187). Accordingly, recombination frequency between @ap@ and the proximally localized D. virilis secretion protein genes @vsp-2@ and @vsp-4@ has been reported to be 2.6% (FBrf0045947 == Aimanova et al., 1987, Dros. Inf. Service 66: 3-5). Recombination between the two genes was not observed and their genetic position calculated to be 144.4. Most probably the two genes are identical to the @Lgp-1@ and @Lgp-3@ genes which are about 2kb apart (FBrf0075968 == Lanio et al., 1994. Biochim Biophys Acta 1219, 576-580). *F \*c 17A1 *F \*x FBrf == 0068564 == Kress, 1993, Chromosoma 102, 734-742 *F ============================================================== *F cat.txt *F \# *F \*a Dvir\a *F \*z FBgn *F \*e approximated *F \*b 5-75.5 *F \*x FBrf0001262 == Metz et al., 1923, Carnegie Inst. Wash. Publ. 328: 1--94 *F \*p two crossveins close together *F \# *F \*a Dvir\ActA1 *F \*z FBgn *F \*c 11A *F \*e actinA1 *F \*M Act5C *F \*x FBrf == Loukas and Kafatos, 1986. Chromosoma 94, 297--308 *F \# *F \*a Dvir\ActD1 *F \*z FBgn *F \*c 38C *F \*e actinD1 *F \*M Act79B *F \*x FBrf == Loukas and Kafatos, 1986. Chromosoma 94, 297--308 *F \# *F \*a Dvir\ActC1 *F \*z FBgn *F \*c 50C *F \*e actinC1 *F \*M Act42A *F \*x FBrf == Loukas and Kafatos, 1986. Chromosoma 94, 297--308 *F \# *F \*a Dvir\ActC2 *F \*z FBgn *F \*c 50B *F \*e actinC2 *F \*M Act57A *F \*x FBrf == Loukas and Kafatos, 1986. Chromosoma 94, 297--308 *F \# *F \*a Dvir\ActE1 *F \*z FBgn *F \*c 27C *F \*e actinE1 *F \*M Act87E *F \*x FBrf == Loukas and Kafatos, 1986. Chromosoma 94, 297--308 *F \# *F \*a Dvir\ActE2 *F \*z FBgn *F \*c 22D *F \*e actinE2 *F \*M Act88F *F \*x FBrf == Loukas and Kafatos, 1986. Chromosoma 94, 297--308 *F \# *F \*a Dvir\ Ax *F \*z FBgn *F \*e Abruptex *F \*b 1-103.3 *F \*x FBrf == Chino, 1941, Dros. Inf. Serv. 14, 44 *F \*p heterozygous female with shortened L veins; wings expanded and thin; few hairs and bristles on head and thorax. Homozygous female and male more extreme *F \# *F \*a Dvir\ap *F \*z FBgn *F \*e apricot *F \*b 1-136.0 *F \*c Distal to 17A1. Recombination frequency between @ap@ and the proximally localized larval glue protein gene @Lgp-1@ is 3% (FBrf 0045117 == Kress, 1986, Naturwissenschaften 73: 180--187). This gene is localized in band 17A1 (FBrf0068564 == Kress, 1993, Chromosoma 102: 734-742). *F \*M g *F \*x FBrf0001836 == Chino, 1929, Jap. J. Genet. 4: 117-131 *F \*p yellowish pink eye *F \# *F \*a Dvir\ap(32) *F \*z FBgn *F \*e apricot-32 *F \*b 1-136.0 *F \*x Demerec (unpublished observation) *F \*p darker than ap *F \# *F \*a Dvir\ar *F \*z FBgn *F \*e abdomen-rotatum *F \*b 6-0.3 *F \*M ar *F \*x Demerec (unpublished observation) *F \*p abdomen rotated *F \# *F \*a Dvir\Aw *F \*z FBgn *F \*e Abnornal wing *F \*b 1-1.4 *F \*x Chino (unpublished observation) *F \*p abnormal flies like supermales of melanogaster; lethal homozygous *F \# *F \*a Dvir\B *F \*z FBgn *F \*e Branched *F \*b 5-141.0 *F \*M px *F \*x Fbrf0001262 == Metz et al., 1923, Carnegie Inst. Wash. Publ. 328:1--94 *F \*p extra veins; not dominant in all crosses *F \# *F \*a Dvir\b *F \*z FBgn *F \*e broken *F \*b 2-188.0 *F \*x FBrf0001262 == Metz et al., 1923, Carnegie Inst. Wash. Publ. 328:1--94 *F \*x FBrf == Chino, 1939, Dros. Inf. Serv. 11, 32 *F \*p both crossveins broken or missing *F \# *F \*a Dvir\Ba *F \*z FBgn *F \*e Baroid *F \*b 4-63.0 *F \*x FBrf == Chino, 1936, Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Japan. J. Genet. 12, 187-210 *F \*p small, rough eyes, homozygous lethal *F \# *F \*a Dvir\ba *F \*z FBgn *F \*e bat *F \*b 3-86.2 *F \*x FBrf0001836 == Chino, 1929, Jap. J. Genet. 4: 117-131 *F \*p wings down-curved *F \# *F \*a Dvir\Bb *F \*z FBgn *F \*e Barb *F \*b 2-80.0 *F \*x FBrf0002006 == Chino, 1930. Jap. J. Genet. 5, 190--197 *F \*p bristles with knot on tip *F \# *F \*a Dvir\Bd *F \*z FBgn *F \*e Beaded *F \*b 5-148.5 *F \*x Chino (unpublished observation) *F \*p wing margins scalloped *F \# *F \*a Dvir\Bh *F \*z FBgn *F \*e Barish *F \*b 4-160.0 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p eyes narrow and ovoid, rough texture, homozygous lethal *F \# *F \*a Dvir\bk *F \*z FBgn *F \*e brick *F \*b 2-248.0 *F \*M ca *F \*x FBrf0002006 == Chino, 1930. Jap. J. Genet. 5, 190--197 *F \*p pink eye colour *F \# *F \*a Dvir\Bl *F \*z FBgn *F \*e Bristle *F \*b 3-30.5 *F \*x FBrf == Chino, 1941. Dros. Inf. Serv. 14, 44 *F \*p wings slightly extended and curved; bristles short and stubby; eyes deformed; homozygous lethal *F \# *F \*a Dvir\bl *F \*z FBgn *F \*e black *F \*b 4-195.0? *F \*M b *F \*x Chino (unpublished observations) *F \*p blackish body color *F \# *F \*a Dvir\Br *F \*z FBgn *F \*e Bar *F \*b 2-64.5? *F \*x FBrf0001836 == Chino, 1929 Jap. J. Genet. 4, 117-131 *F \*p eyes narrow; homozygous lethal; associated with In2a *F \# *F \*a Dvir\Bx *F \*z FBgn *F \*e Beadex *F \*b 1-94.5 *F \*M Beadex *F \*x FBrf0001836 == Chino, 1929 Jap. J. Genet.4, 117-131 *F \*p wing margins scalloped *F \# *F \*a Dvir\C *F \*z FBgn *F \*e Confluent *F \*b 2-45.0 *F \*M Dl *F \*x FBrf0001262 == Metz et al., 1923, Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p L2 veins spread at margin of wing; eyes small, rough *F \*A Delta (Dl) *F \*k L veins fused at wing margin; eyes small, rough; homozygous lethal *F \# *F \*a Dvir\cv *F \*z FBgn *F \*e crossveinless *F \*b 125.0 *F \*M cv *F \*x FBrf0001262 == Metz et al., 1923, Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p posterior crossvein missing *F \*i c *F \# *F \*a Dvir\ca *F \*z FBgn *F \*e claret *F \*b 2-216.1 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5,22 *F \*x FBrf0003578 == Chino, 1936. Jap. J. Genet. 12, 187--210 *F \*p eye colour dark, brownish purple *F \# *F \*a Dvir\cc *F \*z FBgn *F \*e concave *F \*b 2-214.0 *F \*M cmp *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p arista hairs curled *F \# *F \*a Dvir\cd *F \*z FBgn *F \*e cardinal *F \*b 4-32.2 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p vermilion-like eyes *F \# *F \*a Dvir\cf *F \*z FBgn *F \*e coffee *F \*b 3-132.8 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p deep, brown eye colour *F \# *F \*a Dvir\ch *F \*z FBgn *F \*e cherry *F \*b 1-17.0 *F \*x Metz (unpublished observation) *F \*p deep, red eye colour *F \# *F \*a Dvir\cho *F \*z FBgn *F \*e chocolate *F \*b 2-227.3 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p darkish, brown eye colour *F \# *F \*a Dvir\Cl *F \*z FBgn *F \*e Clipped *F \*b 4-68.5? *F \*M dp *F \*x FBrf0002459, also in FBrf0002458 == Lebedeff, 1933. Am. Nat. 67, 69--70 *F \*p cut off wings *F \# *F \*a Dvir\cn *F \*z FBgn *F \*e cinnabar *F \*b 3-9.5 *F \*M scarlet *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p vermilion-like eyes *F \# *F \*a Dvir\ct *F \*z FBgn *F \*e cut *F \*b 1-112.4 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p short, narrow, notched wings *F \# *F \*a Dvir\co *F \*z FBgn *F \*e complex *F \*b 2-178.0 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p extra veins *F \# *F \*a Dvir\cu *F \*z FBgn *F \*e curved *F \*b 4-64.0? *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p wings down-curved *F \# *F \*a Dvir\cv-d *F \*z FBgn *F \*e crossveinless-d *F \*b 5-97.0 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p anterior and posterior crossveins missing *F \# *F \*a Dvir\d *F \*z FBgn *F \*e droop *F \*b 1-150.0 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 23, 1--94 *F \*p wings troop at tip *F \# *F \*a Dvir\dc *F \*z FBgn *F \*e dachsoid *F \*b 5-76.0 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p rounded wings; tarsus 4 jointed *F \# *F \*a Dvir\de *F \*z FBgn *F \*e declined *F \*b 1-34.4 *F \*M wy *F \*x Demerec (unpublished observation) *F \*p wavy wings *F \# *F \*a Dvir\D-cv *F \*z FBgn *F \*e D-crossveinless *F \*b 5-145.0 *F \*x FBrf0005500 == Chino, 1941. Japan. J. Genet. 17, 185-206 *F \# *F \*a Dvir\dh *F \*z FBgn *F \*e dahlia *F \*b 3-81.0 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p dark purplish eye colour *F \# *F \*a Dvir\Dl *F \*z FBgn *F \*e Delta *F \*b 2-45.0 *F \*M Dl *F \*x Lebedeff (unpublished information) *F \*p L veins fused at wing margin; eyes small, rough; homozygous lethal *F \*A Confluent (C) *F \*k L2 veins spread at margin of wing; eyes small, rough *F \# *F \*a Dvir\dl *F \*z FBgn *F \*e dishevelled *F \*b 3-145.0 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p all hairs irregularly directed *F \# *F \*a Dvir\dnc *F \*z FBgn *F \*e dunce *F \*c 13B4 *F \*M dnc *F \*x FBrf0068564 == Kress, 1993. Chromosoma 102, 734--742 *F \# *F \*a Dvir\Ds *F \*z FBgn *F \*e Dominant-short *F \*b 1-100.4 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p distal one-half of L5 obliterated; homozygous lethal *F \# *F \*a Dvir\ds *F \*z FBgn *F \*e dachsous *F \*b 4-173.0 *F \*M dachsous *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p rounded wings; crossveins closer together *F \# *F \*a Dvir\dt *F \*z FBgn *F \*e detached *F \*b 2-255.0 *F \*x Chino (unpublished observation) *F \*p longitudinal veins short *F \# *F \*a Dvir\dw *F \*z FBgn *F \*e dwarfex *F \*b 1-60.0 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Jap. J. Genet. 12, 187--210 *F \*p body and wings small in size *F \# *F \*a Dvir\dy *F \*z FBgn *F \*e dusky *F \*b 1-78-1 *F \*M dusky *F \*x FBrf0002006 == Chino, 1930. Japan. J. Genet. 5, 190--197 *F \*x FBrf0005500== Chino, 1941. Jap. J. Genet. 17, 185--206 *F \*p wings about 4/5 normal size; dusky colour *F \# *F \*a Dvir\eb *F \*z FBgn *F \*e ebony *F \*b 2-83.5 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p very dark body colour *F \# *F \*a Dvir\ec *F \*z FBgn *F \*e echinus *F \*b 1-8.7 *F \*M ec *F \*x FBrf0002006 == Chino, 1930. Japan. J. Genet. 5, 190--197 *F \*p rough eyes *F \# *F \*a Dvir\ep *F \*z FBgn *F \*e expanded *F \*b 3-130.5 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p wings broad, extended *F \# *F \*a Dvir\es *F \*z FBgn *F \*e eosinoid *F \*b 5-91.4 *F \*M brown *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*c 52C *F \*x FBrf0029909 == Evgen'ev, 1977. Dros. Inf. Serv. 52, 59--60 *F \*x FBrf0068564 == Kress, 1993. Chromosoma 102, 734--742 *F \*p dark, purplish eye colour *F \# *F \*a Dvir\ex *F \*z FBgn *F \*e extended *F \*b 5-103.0 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p spread wings *F \# *F \*a Dvir\f *F \*z FBgn *F \*e forked *F \*b 1-89.0 *F \*M f *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 23, 1--94 *F \*p bristles twisted and gnarled *F \# *F \*a Dvir\ft *F \*z FBgn *F \*e fat *F \*b 5-44.5 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p short, broad wings; tarsal joints shortened *F \# *F \*a Dvir\G *F \*z FBgn *F \*e Garnet *F \*b 3-8.0 *F \*M Hn *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 23, 1--94 *F \*p eyes dark purplish colour *F \# *F \*a Dvir\Gl *F \*z FBgn *F \*e Glass *F \*b 5-150.0? *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p facets fused; eye colour diluted; small, rough eyes *F \*A possibly Glued (158.2) *F \# *F \*a Dvir\gl *F \*z FBgn *F \*e glossy *F \*b 6-1.0 *F \*x Moses (unpublished observation) *F \*p small, rough eyes *F \# *F \*a Dvir\go *F \*z FBgn *F \*e golf *F \*b 5-176.5 *F \*x Chino (unpublished observation) *F \*p bristles with knot at tip *F \# *F \*a Dvir\Gp *F \*z FBgn *F \*e Gap *F \*b 6-0.4 *F \*x FBrf0002552 == Chino and Kikkawa, 1933. Genetics 18, 111--116 *F \*p L5 obliterated in middle part; homozygous lethal *F \# *F \*a Dvir\gp-L2 *F \*z FBgn *F \*e gap-L2 *F \*b 3-118.5 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p L2 vein gap *F \# *F \*a Dvir\h *F \*z FBgn *F \*e hunch *F \*b 3-31 *F \*M ascute *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p hunch-back appearance *F \# *F \*a Dvir\hk *F \*z FBgn *F \*e hooked *F \*b 4-62.0 *F \*x Fbrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p bristles barbed or hooked *F \# *F \*a Dvir\ho *F \*z FBgn *F \*e hooked-ocellar *F \*b 1-128.2 *F \*x FBrf001836 == Chino, 1929. Jap. J. Genet. 4, 117--131 *F \*p ocellar bristles hooked *F \# *F \*a Dvir\hp *F \*z FBgn *F \*e hump *F \*b 6-0.5 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p shortened thorax; dark glossy body colour *F \# *F Dvir\hsp82 *F \*z FBgn *F \*e heat shock protein 82 *F \*c 33E *F \*x FBrf0057925 == Lozovskaya et al., 1993, Chromosoma 102, 253-266 *F \*M hsp82 *F \# *F \*a Dvir\hy *F \*z FBgn *F \*e humpy *F \*b 2-135.5 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p thorax humped and glossy; extra wing veins *F \# *F \*a Dvir\i *F \*z FBgn *F \*e interrupted *F \*b 5-95.5? *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p posterior crossvein broken or missing *F \# *F \*a Dvir\in *F \*z FBgn *F \*e incomplete *F \*b 2-45.2 *F \*x FBrf0002006 == Chino 1930, Jap. J. Genet. 5, 190--197 *F \*p L veins, esp. L2, short *F \# *F \*a Dvir\ir *F \*z FBgn *F \*e irregular *F \*b 4-198.0 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p irregular direction of hairs on abdomen and wings *F \# *F \*a Dvir\ix *F \*z FBgn *F \*e intersex *F \*b 3-15.5 *F \*x FBrf0002609, also in FBrf0002608 == Lebedeff, 1934. Am Nat. 68, 68--69 *F \*x FBrf0002861 == Lebedeff, 1934. Proc. Natl. Acad. Sci. USA 20, 613--616 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Jap. J. Genet. 12, 187--210 *F \*p produces development of male system in homozygous females; has no effect on males *F \*i ix(m( (intersex-maleness) *F \*x FBrf0004683 == Lebedeff, 1939. Genetics 24, 553--586 *F \# *F \*a Dvir\Ix(B( *F \*z FBgn *F \*e Intersex-Blanco *F \*b 2-? *F \*x FBrf0005849 == Newby, 1942. Univ. Texas Publ. 4228, 113--145 *F \*x FBrf0005850 == Stone, 1942. Univ. Texas Publ. 4228, 146--152 *F \*p produces development of male system in homozygous females; has no effect on males *F \# *F \*a Dvir\Lb *F \*z FBgn *F \*e Lobster *F \*b 5-118.0 *F \*x Kikkawa (unpublished observation) *F \*p wings and abdomen slightly downcurved; homozygous lethal *F \# *F \*a Dvir\ll *F \*z FBgn *F \*e lanceolate *F \*b 4-3.9 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p narrow, pointed wings *F \# *F \*a Dvir\M *F \*z FBgn *F \*b 6-? *F \*x FBrf0002011 == Demerec, 1930. In: 'The Laws of Life', 45--56, Prague *F \*p increases rate of germinal reversions of @mt-3@ and @mt-5@ *F \# *F \*a Dvir\M-Ia *F \*z FBgn *F \*e Minute-Ia *F \*b 1-18.0 +/- *F \*x Kikkawa (unpublished observation) *F \*p bristles small *F \# *F \*a Dvir\M-2a *F \*z FBgn *F \*e Minute-2a *F \*b 2-218.2 *F \*x Kikkawa (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-2b *F \*z FBgn *F \*e Minute-2b *F \*b 2-220.6 *F \*x Kikkawa (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-2c *F \*z FBgn *F \*e Minute-2c *F \*b 2-243.5 *F \*x Kikkawa (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-2d *F \*z FBgn *F \*e Minute-2d *F \*b 2-168.0 *F \*x FBrf == Chino, 1940. 13, 62--63 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-2e *F \*z FBgn *F \*e Minute-2e *F \*b 2-256.6 *F \*x FBrf == Chino, 1940. 13, 62--63 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-2f *F \*z FBgn *F \*e Minute-2f *F \*b 2-257.6 *F \*x FBrf == Chino, 1940. 13, 62--63 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-3c *F \*z FBgn *F \*e Minute-3c *F \*b 3-84.2 *F \*x FBrf == Chino, 1940. 13, 62--63 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-4a *F \*z FBgn *F \*e Minute-4a *F \*b 4-36.0? *F \*x Kikkawa (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-4b *F \*z FBgn *F \*e Minute-4b *F \*b 4-161.0 *F \*x Chino (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-4c *F \*z FBgn *F \*e Minute-4c *F \*b 4-156.0 *F \*x Chino (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-4d *F \*z FBgn *F \*e Minute-4d *F \*b 4-22.6 *F \*x Chino (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-4e *F \*z FBgn *F \*e Minute-4eb *F \*b 4-158.8 *F \*x Kikkawa (unpublished observation) *F \*p minute bristles *F \# *F \*a Dvir\M-4f *F \*z FBgn *F \*e Minute-4f *F \*b 4-22.9 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Japan. J. Genet. 12, 187--210 *F \*p minute bristles *F \# *F \*a Dvir\M-4g *F \*z FBgn *F \*e Minute-4g *F \*b 4-82.5 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-4h *F \*z FBgn *F \*e Minute-4h *F \*b 4-165.0 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-4i *F \*z FBgn *F \*e Minute-4i *F \*b 4-25.1 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p minute bristles; homozygous lethal; extra veins near tip of L2, L5 *F \# *F \*a Dvir\M-4j *F \*z FBgn *F \*e Minute-4j *F \*b 4-153.2 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-4k *F \*z FBgn *F \*e Minute-4k *F \*b 4-17.3 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p minute bristles; homozygous lethal *F \# *F \*a Dvir\M-5a *F \*z FBgn *F \*e Minute-5a *F \*b 5-101.0 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Japan. J. Genet. 12, 187--210 *F \*p minute bristles *F \# *F \*a Dvir\M-5b *F \*z FBgn *F \*e Minute-5b *F \*b 5-144.2 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p minute bristles *F \# *F \*a Dvir\m *F \*z FBgn *F \*e magenta *F \*b 1-83.5 *F \*M rb *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p dark purplish eye colour *F \*A m(a( (magenta-alpha) *F \*x FBrf0001618 == Demerec, 1927. Proc. Natl. Acad. Sci. USA 13, 249-253 *F \*x FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*k unstable, reverts to normal *F \# *F \*a Dvir\mh *F \*z FBgn *F \*e mahogany *F \*b 5-140.4 *F \*x FBrf0002006 == Chino, 1930. Japan. J. Genet. 5, 190--197 *F \*p deep brown eye colour *F \# *F \*a Dvir\mi *F \*z FBgn *F \*e minus *F \*b 2-? *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p one or more thoracic bristles *F \# *F \*a Dvir\mo *F \*z FBgn *F \*e mottled *F \*b 2-89.0 *F \*x FBrf == Chino, 1941. Dros. Inf. Serv. 14, 44 *F \*p eye colour red with small dark spots; female sterile *F \# *F \*a Dvir\mr *F \*z FBgn *F \*e morula *F \*b 5-? *F \*x Kikkawa (unpublished observation) *F \*p rough eye; bristles occasionally missing *F \# *F \*a Dvir\Ms *F \*z FBgn *F \*e Missing *F \*b 4-63.0 *F \*x Chino (unpublished observation) *F \*p bristles and hairs on head and thorax missing; males sterile *F \# *F \*a Dvir\mt *F \*z FBgn *F \*e miniature *F \*b 1-78.0 *F \*M miniature *F \*x FBrf0001512 == Demerec, 1926. Proc. Natl. Acad. Sci. USA 12, 687--690 *F \*p miniature wings very dusky in colour, about 2/3 of normal size *F \*A mt(2( *F \*k wings as long as abdomen *F \*x Moses (unpublished observation) *F \*A mt(3( *F \*k unstable, reverts to normal; wings like mt1 *F \*E FBrf0001512 == Demerec, 1926. Proc. Natl. Acad. Sci. USA 12, 687--690 *F \*A mt(3a( (miniature(3(-alpha) *F \*k unstable in both somatic and germinal cells *F \*x FBrf0001512 == Demerec, 1929. Proc. Natl. Acad. Sci. USA 15, 870--876 *F \*x FBrf0005256 == Demerec, 1941. Cold Spring. Harb. Symp. Quant. Biol. 9, 145--150 *F \*A mt(3b( (miniature(3(-beta) *F \*k stable *F \*E FBrf0001512 == Demerec, 1929. Proc. Natl. Acad. Sci. USA 15, 870--876 *F \*A mt(3c( (miniature(3(-gamma) *F \*k unstable in soma only *F \*E FBrf0001512 == Demerec, 1929. Proc.Natl. Acad. Sci. USA 15, 870--876 *F \*A mt(4( (miniature(4() *F \*k wings about4/5 of normal size *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*A mt(5( (miniature(5() *F \*k unstable, reverts to normal; wings like mt(2( *F \*E FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*A mt(5a( (miniature(5(-alpha) *F \*k unstable as mt(3a( *F \*E FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*A mt(5b( (miniature(5(-beta) *F \*k unstable as mt(3b( *F \*E FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*A mt(5c( (miniature(5(-gamma) *F \*k unstable as mt(3c( *F \*E FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*A mt(6( (miniature(6() *F \*k wings about ½ normal size *F \*E FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*A mt(7( (miniature(7() *F \*k wings reduced in size as mt *F \*x Kikkawa (unpublished observation) *F \# *F \*a Dvir\N *F \*z FBgn *F \*e Notched *F \*b 1-102.9 *F \*M N *F \*x Chino (unpublished observation) *F \*p tips of wings broken; deficiency involving white locus; homozygous lethal *F \*c 13B7 *F \*x FBrf0068564 == Kress, 1993. Chromosoma 102, 734--742 *F \# *F \*a Dvir\N-3a *F \*z FBgn *F \*e Notched-3a *F \*b 3-83.0 *F \*x Chino (unpublished observation) *F \*p tips of wings broken; homozygous lethal *F \# *F \*a Dvir\na *F \*z FBgn *F \*e nail *F \*b 2-35.7 *F \*x FBrf == Chino, 1941. Dros. Inf. Serv. 14, 44 *F \*p all bristles short and hook or barb on tip *F \# *F \*a Dvir\nd *F \*z FBgn *F \*e needle *F \*b 1-10.4 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p prickly-like bristle *F \# *F \*a Dvir\ne *F \*z FBgn *F \*e nebulous *F \*b 2-227.0 *F \*x Kikkawa (unpublished observation) *F \*p thin wings with glitter *F \# *F \*a Dvir\ni *F \*z FBgn *F \*e nick *F \*b 4-19.7 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62-63 *F \*p tip of wings nicked *F \# *F \*a Dvir\norpA *F \*z FBgn *F \*e no receptor potential *F \*c 9C *F \*x FBrf0000057925 == Lozovskaya et al., 1993. Chromosoma 102, 253-266 *F \*M norpA *F \# *F \*a Dvir\p *F \*z FBgn *F \*e pink *F \*b 2-212.5 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p pink eye *F \# *F \*a Dvir\pc *F \*z FBgn *F \*e polychaeta *F \*b 5-113.0 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p extra bristles; eyes rough *F \# *F \*a Dvir\Pcp *F \*z FBgn *F \*e Pupal cuticle protein *F \*c 49A2 *F \*x FBrf0057925 == Lozovskaya et al., 1993, Chromosoma 102, 253-266 *F \*M Pcp *F \# *F \*a Dvir\pe *F \*z FBgn *F \*e peach *F \*b 5-203.0 *F \*x Moses (unpublished observation) *F \*p yellowish pink eye *F \# *F \*a Dvir\Pi *F \*z FBgn *F \*e Pincer *F \*b 2-79.6 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p wings short and bent inside; males more extreme; homozygous lethal *F \# *F \*a Dvir\pi *F \*z FBgn *F \*e pointed *F \*b 6-1.0 *F \*x FBrf == Chino, 1941. Dros. Inf. Serv. 14, 44 *F \*p wings narrow and pointed *F \# *F \*a Dvir\pk *F \*z FBgn *F \*e prickly *F \*b 5-27.0 *F \*x Chino (unpublished observation) *F \*p prickle-like bristles *F \# *F \*a Dvir\pr-b *F \*z FBgn *F \*e prickly-b *F \*b 5-0.0 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p similar to pk, but not allelic *F \# *F \*a Dvir\pm *F \*z FBgn *F \*e plum *F \*b 4-18.7 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p eye darkish brown *F \# *F \*a Dvir\po *F \*z FBgn *F \*e port *F \*b 5- 151.0 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p eye colour deep pink *F \# *F \*a Dvir\ps *F \*z FBgn *F \*e persimmon *F \*b 2-151.5 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p eye colour darkish purple *F \# *F \*a Dvir\Pu *F \*z FBgn *F \*e Puffed *F \*b 2-64.5 *F \*x Farrow (unpublished observations) *F \*p rough, small eyes *F \# *F \*a Dvir\pw *F \*z FBgn *F \*e pink-wing *F \*b 5-136.0 *F \*x Chino (unpublished observations) *F \*p wings shorter; deep pinkish eye colour *F \# *F \*a Dvir\px *F \*z FBgn *F \*e plexus *F \*b 4-0.0 *F \*M net *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p extra wing veins *F \# *F \*a Dvir\R *F \*z FBgn *F \*e Rounded *F \*b 2-214.0 *F \*x FBrf0001580 == Demerec, 1928. Int. Kong.Vererb. Supple.Bd. 1, 183--193 *F \*x FBrf0002434 == Lebedeff, 1932. Proc. Natl. Acad. Sci. USA 18, 343--349 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p wings retracted in marginal portion between L3 and L5 *F \# *F \*a Dvir\r *F \*z FBgn *F \*e rugose *F \*b 1-127.0 *F \*M lozenge *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p rough, slightly yellowish eye *F \# *F \*a Dvir\ra *F \*z FBgn *F \*e raspberry *F \*b 2-223.5 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p dark, ruby-like eye colour *F \# *F \*a Dvir\rc *F \*z FBgn *F \*e reduced *F \*b 4-176.5 *F \*M reduced *F \*z Chino (unpublished observations) *F \*p minute bristles; female sterile *F \# *F \*a Dvir\rd *F \*z FBgn *F \*e rudimentary *F \*b 1-122.6 *F \*M rudimentary *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p small wings *F \# *F \*a Dvir\re *F \*z FBgn *F \*e red *F \*b 3-63.0 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p reddish eye colour *F \# *F \*a Dvir\rg *F \*z FBgn *F \*e ragged *F \*b 1-160.0 *F \*M cut *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p wings notched in margin *F \# *F \*a Dvir\rl *F \*z FBgn *F \*e rolled *F \*b 3-0.0 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p wing edges rolled *F \# *F \*a Dvir\Rp49 *F \*z FBgn *F \*e ribosomal protein 49, large subunit *F \*c 22E *F \*x FBrf 0057925 == Lozovskaya et al., 1993, Chromosoma 102, 253-266 *F \# *F \*a Dvir\ro2a *F \*z FBgn *F \*e rough 2a *F \*b 2-230.0 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p eyes rough, slightly small in size *F \# *F \*a Dvir\ro2b *F \*z FBgn *F \*e rough 2b *F \*b 2-210.0 *F \*x Kikkawa (unpublished observations) *F \*p eyes small, rough *F \*k phenotypic data attached to an allele *F \# *F \*a Dvir\ro2c *F \*z FBgn *F \*e rough 2c *F \*b 2-231.0 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p eyes slightly rough *F \# *F \*a Dvir\ro5a *F \*z FBgn *F \*e rough 5a *F \*b 5-145.0 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p eyes rough, slightly larger *F \# *F \*a Dvir\rr *F \*z FBgn *F \*e retracted *F \*b 2-243.3 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p wings short and broad *F \# *F \*a Dvir\rs *F \*z FBgn *F \*e rose *F \*b 3-40.5 *F \*x Chino (unpublished observations) *F \*p purplish, pink eye colour; males sterile *F \# *F \*a Dvir\rt *F \*z FBgn *F \*e russet *F \*b 2-162.0 *F \*x FBrf == Chino, 1935. Dros. Inf. Serv. 3, 41 *F \*p dark eye colour *F \# *F \*a Dvir\ru *F \*z FBgn *F \*e ruffled *F \*b 5-44.2 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p dorso-central bristles and hairs are curled *F \# *F \*a Dvir\s *F \*z FBgn *F \*e short *F \*b 1-114.0 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p L5 is obliterated in distal part *F \# *F \*a Dvir\S-1 *F \*z FBgn *F \*b 2-? *F \*x FBrf0001863 == Demerec, 1929. Proc. Natl. Acad. Sci. USA 15, 834--838 *F \*p increases rate of somatic reversions of mt3 and mt5 *F \*i S1 *F \# *F \*a Dvir\s-2 *F \*z FBgn *F \*b 5-? *F \*x FBrf0001863 == Demerec, 1929. Proc. Natl. Acad. Sci. USA 15, 834--838 *F \*p same as S-1 *F \# *F \*a Dvir\S-3 *F \*z FBgn *F \*b 4 or 6-? *F \*x FBrf0001863 == Demerec, 1929. Proc. Natl. Acad. Sci. USA 15, 834--838 *F \*p same as S-1 *F \# *F \*a Dvir\S-4 *F \*z FBgn *F \*b 3 or 5-? *F \*x FBrf0005256 == Demerec, 1941. Cold Spring Harbor Symp. Quant. Biol. 9, 145--150 *F \*p same as S-1 *F \# *F \*a Dvir\sa *F \*z FBgn *F \*e small wings *F \*b 4-49.3 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5,22 *F \*x FBrf0003578 == Chino, 1936. Japan. J. Genet. 12, 187--210 *F \*p wings about 80% normal size *F \# *F \*a Dvir\sal *F \*z FBgn *F \*e salmon *F \*b 3-19.6 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p reddish eye colour *F \# *F \*a Dvir\Sb *F \*z FBgn *F \*e Stubble *F \*b 5-44.5 *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p bristles cut off; homozygous lethal *F \# *F \*a Dvir\sb *F \*z FBgn *F \*e small bristles *F \*b 1-131.5 *F \*M tiny *F \*x Metz (unpublished observations) *F \*p bristles and hairs small *F \# *F \*a Dvir\Sc *F \*z FBgn *F \*e Scutellar *F \*b 4-27.0 *F \*x Kikkawa (unpublished observations) *F \*p bristles, esp. scutellar, are missing *F \# *F \*a Dvir\se *F \*z FBgn *F \*e sepia *F \*b 1-0.1 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p dark eye colour *F \# *F \*a Dvir\sh *F \*z FBgn *F \*e shaggy *F \*b 5-62.5 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p hairs on abdomen and tarsus are irregularly directed *F \# *F \*a Dvir\sk *F \*z FBgn *F \*e spike *F \*b 4-8.2 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*p bristles short and stubby *F \# *F \*a Dvir\sl *F \*z FBgn *F \*e slender *F \*b 2-183.5 *F \*x Kikkawa (unpublished observations) *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p minute bristles *F \# *F \*a Dvir\sl-b *F \*z FBgn *F \*e slender-b *F \*b 2-0.0 *F \*x FBrf0005500 == Chino, 1941. Japan. J. Genet. 17, 185--206 *F \*x FBrf == Chino, 1941. Dros. Inf. Serv. 14, 44 *F \*p similar to sl *F \# *F \*a Dvir\sm *F \*z FB *F \*e semiplexus *F \*b 5-174.0 *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p extra veins *F \# *F \*a Dvir\sp *F \*z FBgn *F \*e spread *F \*b 3-106.5 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p spread wings *F \# *F \*a Dvir\sq *F \*z FBgn *F \*e squat *F \*b 4-20.3 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Japan. J. Genet. 12, 187-210 *F \*p small, rounded wings; homozygous sterile *F \# *F \*a Dvir\ss *F \*z FBgn *F \*e spineless *F \*b 4-40.0 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 7, 74--75 *F \*x FBrf == Chino, 1937. Dros. Inf. Serv. 8, 61 *F \*p all bristles short *F \# *F \*a Dvir\St *F \*z FBgn *F \*e Star *F \*b 4-84.0 *F \*x Kikkawa (unpublished observations) *F \*p small, rough eyes; homozygous lethal *F \# *F \*a Dvir\st *F \*z FBgn *F \*e scarlet *F \*b 5-67.5 *F \*M cinnabar *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117-131 *F \*c 51CD *F \*x FBrf0029909 == Evgen'ev, 1977. Dros. Inf. Serv. 52, 59--60 *F \*x FBrf0068564 == Kress, 1993. Chromosoma 102, 734--742 *F \*p vermilion-like eyes *F \# *F \*a Dvir\sv *F \*z FBgn *F \*e short veins *F \*b 3-24.5 *F \*M veinlet *F \*x Raleigh (unpublished observations) *F \*p all veins except L1 are short *F \# *F \*a Dvir\Sv-5 *F \*z FBgn *F \*e Short vein 5th *F \*b 5-127.5 *F \*x Raleigh (unpublished observations) *F \*p L5 short *F \# *F \*a Dvir\sw *F \*z FBgn *F \*e straw *F \*b 5-147.0 *F \*M straw *F \*x Chino (unpublished observations) *F \*p yellowish body colour *F \# *F \*a Dvir\sy *F \*z FBgn *F \*e stubby *F \*b 6-0.8 *F \*M shaven *F \*x FBrf0002552 == Chino and Kikkawa, 1933. Genetics 18, 111--116 *F \*p Stubble-like bristles *F \# *F \*a Dvir\T *F \*z FBgn *F \*e Triangle *F \*b 1-104.5 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p veins thickened and knotted *F \# *F \*a Dvir\t *F \*z FBgn *F \*e telescoped *F \*b 3-57.5 *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p shortened thorax; small eyes *F \# *F \*a Dvir\tb *F \*z FBgn *F \*e tiny bristles *F \*b 3-104.0 *F \*x Chino (unpublished observations) *F \*p minute bristles *F \# *F \*a Dvir\v *F \*z FBgn *F \*e vermilion *F \*b 1-25.5 *F \*M vermilion *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p bright eye colour *F \# *F \*a Dvir\va *F \*z FBgn *F \*e varnished *F \*b 2-231.5 *F \*M glass *F \*x FBrf == Chino, 1939. Dros. Inf. Serv. 11, 32 *F \*p small rough eye with reddish colour *F \# *F \*a Dvir\vg *F \*z FBgn *F \*e vestigial *F \*b 5-131.0 *F \*M vestigial *F \*x Chino (unpublished observations) *F \*p wings and balancers reduced to vestiges *F \# *F \*a Dvir\ve *F \*z FBgn *F \*e veinlet *F \*b 4-81.7 *F \*x FBrf == Chino, 1936. Dros. Inf. Serv. 5, 22 *F \*x FBrf0003578 == Chino, 1936. Japan. J. Genet. 12, 187--210 *F \*p L2, 4 and 5 wing veins short *F \# *F \*a Dvir\vl *F \*z FBgn *F \*e vestigial-like *F \*b 4-116.0? *F \*x FBrf0001836 == Chino, 1929. Japan. J. Genet. 4, 117--131 *F \*p rudimentary wings *F \# *F \*a Dvir\vs *F \*z FBgn *F \*e vesiculated *F \*b 1-27.0 *F \*M vesiculated *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*p vesicles in wings *F \# *F \*a Dvir\w *F \*z FBgn *F \*e white *F \*b 1-105.0 *F \*M w *F \*x Weinstein (unpublished observations) *F \*c 13C1 *F \*x FBrf0057925 == Lozovskaya et al., 1993. Chromosoma 102, 253-266 *F \*x FBrf0068564 == Kress, 1993. Chromosoma 102, 734-742 *F \*p white eye colour *F \# *F \*a Dvir\we *F \*z FBgn *F \*e wee *F \*b 1-72.0 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p wings thin, short and narrow *F \# *F \*a Dvir\y *F \*z FBgn *F \*e yellow *F \*b 1-2.9 *F \*M yellow *F \*x FBrf0001262 == Metz et al., 1923. Carnegie Inst. Wash. Publ. 328, 1--94 *F \*c 1D *F \*x FBrf0057925 == Lozovskaya et al., 1993. Chromosoma 102, 253-266 *F \*p yellow body colour; hairs and bristles gay *F \# *F \*a Dvir\yre-a *F \*z FBgn *F \*e reddish-alpha *F \*x FBrf0001699 == Demerec, 1928. Genetics 13, 359-388 *F \*x FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*p unstable; reverts to normal in heterozygous females. Body and hairs and bristles more yellowish than @y@ *F \# *F \*a Dvir\yre-1 *F \*z FBgn *F \*e reddish-1 *F \*x FBrf0001699 == Demerec, 1928. Genetics 13, 359-388 *F \*x FBrf0005256 == Demerec, 1941. Cold Spring Harb. Symp. Quant. Biol. 9, 145--150 *F \*p stable; colour like @yre-a@ *F \# *F \*a Dvir\Yh *F \*z FBgn *F \*e Yellowish *F \*b 4-115.0 *F \*x FBrf == Chino, 1940. Dros. Inf. Serv. 13, 62--63 *F \*p pale yellowish body colour; tip of bristles colourless; homozygous lethal *F \# *F # *U FBrf0089316 *a Calleja *b M. *t 1996.10.16 *T personal communication to FlyBase *u *F Archived. # *U FBrf0089317 *a Bunch *b T. *t 1996.10.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0089488 *a Ropp *b P. *t 1996.9.23 *T personal communication to FlyBase *u *F From ropp@niehs.nih.gov Mon Sep 23 15:34:24 1996 *F Date: Mon, 23 Sep 1996 10:22:12 -0400 *F From: 'Ropp.Philip' *F Subject: DNA Polymerase Gamma *F To: Michael Ashburner *F Mime-Version: 1.0 *F X-Mailer: Mail*Link SMTP-MS 3.0.2 *F Content-Type: TEXT/PLAIN; CHARSET=US-ASCII *F Content-Transfer-Encoding: 7BIT *F Content-Length: 510 *F *F Dear Dr. Ashburner, *F *F In response to your letter of September 13, 1996, the Drosophila melanogaster *F gene encoding DNA polymerase gamma that we describe in Genbank (accession *F number U60298) is the genomic sequence for the catalytic subunit (125 kDa) of *F the mitochondrial DNA polymerase. *F *F One note, your first reference that you included: *F *F DNApol-gamma 120 *F Chiang et al., 1993, Proc. Natl. Acad. Sci. USA 90(19): 9105-9109 *F *F is not for DNA polymerase gamma but is for DNA polymerase delta. *F *F Sincerely, *F Philip Ropp # *U FBrf0089922 *a Ariga *b H. *t 1996.9.17 *T personal communication to FlyBase *u *F >From hiro@yakugaku.pharm.hokudai.ac.jp Tue Sep 17 05:12:57 1996 *F Date: Tue, 17 Sep 1996 13:05:54 +0900 (JST) *F To: m.ashburner@gen.cam.ac.uk *F From: hiro@yakugaku.pharm.hokudai.ac.jp (hiroyoshi ariga) *F Subject: D43795 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset=iso-2022-jp *F X-Mailer: Eudora-J(1.3.8.5-J13) *F Content-Length: 471 *F *F Dr. Michael Ashburner *F University of Cambridge Department of genetics *F Downing Street, Cambridge, CB2 3EH *F England *F Sept. 19, 1996 *F *F Dear Dr. Ashburner, *F *F Thank you for your letter about our cDNA, D43795. *F Name of cDNA is CP-1. This has no structural motif we know so far. We say *F that CP-1 is a transcription factor recognizing CAAT motif. *F *F Thank you, *F Hiroyoshi Ariga *F Faculty of Pharmaceutical Sciences, Hokkaido University *F Kita 12, Nishi 6, Kita-ku, Sapporo 060 *F Japan # *U FBrf0089931 *a Kernan *b M. *t 1996.7.25 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Jun 25 15:15:59 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 25 Jun 96 15:15:55 BST *F To: mkernan@epo.som.sunysb.edu *F Subject: Help FlyBase - larval touch insensitive lines *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 749 *F Hi Dr. Kernan, *F I am curating this paper of yours for FlyBase: *F FBrf0073546 == Kernan et al., 1994, Neuron 12(6): 1195--1206 *F in which you list many larval touch insensitive lines in Table 1 (page *F 1198). *F Some of these you have assigned to complementation groups. My question is *F about the others. Have you assigned any of the remaining lines (e.g. 375, *F 70, 376 ...) to known complementation groups yet? *F If so, could you please provide me with the relevant gene symbols. *F If not, then we shall have to give these an arbitrary gene symbol, eg lbh1, *F lbh2 etc, (for larval behaviour gene 1, 2 etc) so I can capture data *F concerning the larval, adult and mosaic phenotypes. *F Many thanks for your help, *F Eleanor Whitfield *F FlyBase *F From mkernan@epo.som.sunysb.edu Wed Jul 24 20:39:30 1996 *F Date: Wed, 24 Jul 1996 15:30:44 -1000 *F From: Maurice Kernan *F Reply-To: mkernan@epo.som.sunysb.edu *F Organization: SUNY at Stony Brook *F X-Mailer: Mozilla 2.01 (Macintosh; I; 68K) *F Mime-Version: 1.0 *F To: 'Eleanor Whitfield (Genetics)' *F Subject: Re: Help FlyBase - larval touch insensitive lines *F Content-Type: text/plain; charset=us-ascii *F Content-Transfer-Encoding: 7bit *F Content-Length: 1172 *F Re: larval touch insensitives *F Hello Eleanor *F Sorry for the delay in replying to your inquiry - I have *F been away for 3 weeks teaching a summer course and am only *F now catching up on email. To answer your question about the *F lines mentioned in our Neuron paper - no more information, *F complementation or otherwise, is available than is presented *F in that table. (We have been concentrating on the unc and *F uncl -type mutations, for which we can define an *F electrophysiological phenotype.) If you wish to assign *F symbols to the lines (remembering that these are *F mutagenized chromosomes, rather than identified loci), how *F about til(touch-insensitive-larva)E, F, G...., for *F consistency with those that we have already named? tilA, by *F the way, has been lost. *F I appreciate all your efforts in curating the database - *F keep up the good work.... *F Maurice Kernan *F From eleanor@gen.cam.ac.uk Thu Jul 25 08:24:34 1996 *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 25 Jul 96 08:24:31 BST *F To: mkernan@epo.som.sunysb.edu *F Subject: Re: Help FlyBase - larval touch insensitive lines *F Cc: eleanor@gen.cam.ac.uk *F Content-Length: 1214 *F Hi Maurice, *F >If you wish to assign symbols to the lines, how about *F >til(touch-insensitive-larva)E, F, G...., for consistency with those that we *F >have already named? *F that is fine, so this is the designation I shall be giving each line: *F line 375 tilE *F line 70 tilF *F line 376 tilG *F line 75 tilH *F line 411 tilI *F line 366 tilJ *F line 147 tilK *F line 350 tilL *F line 310 tilM *F line 301 tilN *F line 317 tilO *F line 78 tilP *F each shall have an allele number 1 so the larval, adult and mosaic *F phenotypes can be captured. *F > I appreciate all your efforts in curating the database - *F >keep up the good work.... *F Thank you very much. *F I shall curate this correspondance as a personal communication from you. *F Look forward to hearing from you soon, *F Eleanor Whitfield # *U FBrf0089933 *a Roote *b J. *t 1996.9.11 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Sep 11 16:09:14 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 11 Sep 1996 16:01:45 +0100 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: update *F Content-Length: 210 *F Hi Aubrey, *F Here is a snippett of info: *F tup1 complements Df(2L)TW1, pr-A14 and DS8. *F Cheers, *F John # *U FBrf0089969 *a Ashburner *b M. *t 1992.5.27 *T personal communication to FlyBase *u *F Archived. # *U FBrf0089992 *a Roote *b J. *t 1996.9.18 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Tue Sep 17 15:25:50 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 17 Sep 1996 15:18:20 +0100 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: el94 *F Content-Length: 141 *F Aubrey, *F Df(2L)el94 is according to Siegrun associated with In(2L)35B;36D. The *F deletion is not visible. Presumably a 3 break event. *F el94 has not been mapped molecularly, but complementation pattern suggests *F it is at least pu- elA- (rather than elB- pu-). It may be elB- also, but *F we can't tell easily. *F John # *U FBrf0089993 *a Coyne *b J.A. *t 1996.9.20 *T personal communication to FlyBase *u *F From jcoyne@pondside.uchicago.edu Fri Sep 20 15:30:27 1996 *F Date: Fri, 20 Sep 1996 09:22:15 -0600 *F To: rd120@gen.cam.ac.uk *F From: jcoyne@pondside.uchicago.edu (Jerry Coyne) *F Subject: New mutation in D. mauritiana for FlyBase *F Content-Length: 846 *F Dear Rachel, *F I'd like to add a new D. mauritiana mutation to FlyBase. I've sent the *F stock to Kay Yoon at the Species Center in Bowling Green. *F I call the mutation 'rough eye 1' (re1). It maps roughly to position *F 28 on the X chromosome. The phenotype is irregular facets of the eyes, eyes *F frequently reduced, and often an indented or kidney shape to the eye. *F Stock very weak, probably because of low female fertility. Probably the *F same as D. melanogaster *F 'kidney eye' (ke; 1-28.6) which is also semisterile, but we couldn't *F determine this as ke has been lost in melanogaster. *F Thanks, *F Jerry *F __________________________________ *F Jerry Coyne *F Department of Ecology & Evolution *F The University of Chicago *F 1101 E. 57th Street *F Chicago, IL 60637 USA *F Phone: (312) 702-1105 or 702-1106 *F Fax: (312) 702-9740 *F email: jcoyne@pondside.uchicago.edu # *U FBrf0089994 *a van Doren *b M. *t 1996.9.20 *T personal communication to FlyBase *u *F Date: Mon, 12 Aug 1996 18:38:55 +30000 *F From: Mark Van Doren *F Use of the heat shock head involution defective (hid) conditional lethal *F chromosomes. The heat shock hid transgenes were generated by Meagen Grether *F in Herman Steller's lab (Genes Dev. 1995 9;1694). They were first adopted *F for use in genetic screens by Lisa Moore in Ruth Lehmann's lab, and were *F jumped onto balancer chromosomes by Mark Van Doren, also in Ruth's lab: *F CyO, P{w+; HShid4} *F TM3, Sb, P{w+; HShid14} *F In order to eliminate heat shock hid chromosome containing offspring, we *F have successfully used the following protocol: *F \-let parents lay for 5 days in vials at room temp or 25C *F \-remove parents *F \-heat shock for 2 hrs. at 37C in a water bath on day 5 *F \-repeat heat shock on day 6 *F Notes: *F Heat shocks must be done in a water bath. We have had no success with *F using air incubators. Large water baths can be made from virtually any *F large container along with a mountable heating circulation pump. *F We have done the heat shocks in plastic vials. Using glass or using *F bottles may require a slight modification of the heat shock protocol. *F We have not extensively altered heat shock conditions. For the *F particular insertion line and genetic background originally tested, heat *F shock on two successive days reduced the number of escapers compared to *F single heat shock. Other insertion lines and genetic backgrounds have *F proved even more effective, and it is possible that a single heat shock *F might be sufficient in some cases. *F Please contact Mark Van Doren with any *F questions or, importantly, with any suggestions or improvements on using *F these reagents. # *U FBrf0089995 *a Roote *b J. *t 1996.9.24 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Tue Sep 24 14:49:43 1996 *F Date: Tue, 24 Sep 1996 14:42:02 +0100 *F Hi folks, *F We found that A507 was mutant for chiffon. This doesn't quite match with *F the insertion site (chif is at 35F-36A), but close enough to be *F interesting. *F Insertion_name : P{ry+t7.2=lArB}A507.2M2 *F IU Stock : B-#P1033 *F Genotype : CyO, P{ry+t7.2=lArB}A507.2M2/b1 Adh* cn* l(2)*; *F ry506 *F Polytene : 35D-E *F A.K.A. : \#WG1033 *F Pattern : embryo: pole cells, brain, posterior spiracles; ovary: no *F staining *F at stock center : 8/01/1990 *F Donor : HHMI P Stock Center *F Donor'sSource : Walter Gehring *F John # *U FBrf0089999 *a Freeman *b M. *t 1996.10.3 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Matthew Freeman, MRC Laboratory of Molecular Biology *F To: Bloomington Drosophila Stock Center *F Subject: GMR-GAL4 *F Date: 3 October 1996 *F *F Background: Transposon insertion now at Bloomington but not in FlyBase. *F *F Information communicated: *F Freeman, M. (1996). Reiterative use of the EGF receptor triggers differentiation of all cell types in the Drosophila eye. Cell, in press. *F *F Symbol used by donor P[w[+], GMR-Gal4 #12] *F Map location chromosome 2 *F Phenotype Gal4 is driven by the glass enhancer, and is the strongest way of expressing things behind the furrow in the eye disc. *F Other info from donor: The GMR-Gal4 line we made by cloning the Gal4 gene into pGMR1 (made in Gerry Rubin's lab). Most transformant *F lines had rough eyes, even as heterozygotes, but line 12 is smooth-eyed when heterozygous and grown at 25o or below. # *U FBrf0090007 *a Zhang *b Y. *t 1996.10.14 *T personal communication to FlyBase *u *F >From yqz@mole.bio.cam.ac.uk Mon Oct 14 13:30:45 1996 *F Date: Mon, 14 Oct 1996 13:26:11 +0100 (BST) *F From: 'Yong Qing Zhang (Genetics)' *F To: m.ashburner@gen.cam.ac.uk *F Subject: remapping of histone H3.3 gene *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 277 *F The clone p82BR containing full-length H3.3B genomic gene from D. *F melanogaster(from Anna Akhmanova in Wolfgang Hennig labotatory) was in *F situed on D. melanogaster Canton S chromosomes. The band lit up is not 9D as *F described in Anna's paper(Genome,38:586--600), but at 8C-D. # *U FBrf0090024 *a Rutherford *b S.L. *t 1996.11.4 *T personal communication to FlyBase *u *F >From srutherf@midway.uchicago.edu Mon Nov 4 21:49:50 1996 *F Date: Mon, 4 Nov 1996 15:31:03 -0600 *F To: Michael Ashburner (Genetics) *F From: srutherf@midway.uchicago.edu (Suzanne Rutherford) *F X-Sender: srutherf@ucpopmail.uchicago.edu *F Subject: Re: Cyclophylin 1 *F Content-Length: 3290 *F Dear M- *F By in situ hybridization Cyp-1 maps to 14B11-12. Using my Cyp-1 *F mutant and measuring protein levels Dp(1;4)r+,Df(1)81h24b does not cover *F Cyp-1; Dp(1;4)r+,Df(1)81j23a does cover Cyp-1 (this is also very clear by *F the cytology. Based on molecular mapping by Jones and Rubin (1990) *F Df(1)l32 maps proximal to the coding region 5' of the gene but by Western *F blot and CsA binding this Df may not cover Cyp and would be the closest *F defining breakpoint. Df(1)E150 covers Cyp-1. The gene is transcribed from *F proximal to distal. There is a 1.5 Kb intron in the gene between codon *F 23 and 24 of the 165 aa of the protein. and at least two or (probably) *F three polyA sites used (0.9, 1.0 and 1.3 Kb)- the Northern published by *F Stamnes did not resolve the 0.9 and 1 Kb bands but I find the other polyA *F site in the sequence and Reynolds and Tanouye reported the three *F transcripts at the last mmm fly meeting in Chicago. The genomic sequence *F of 10Kb around Cyp-1 is in my thesis but may never have been submitted to *F Genbank. *F Jones, K.R. and Rubin, G.M. (1990) *F Molecular analysis of no-on-transient A, a gene required for normal vision *F in Drosophila. *F Neuron 4:711-23 *F I'll fax a detailed map of 14B and Cyp-1 and a complementation *F table from my thesis they may help you. Can you reference my thesis? It *F is on microfilm: *F 'The Genetic and Biochemical Characterization of the Major Cyclophilin *F Isoform in Drosophila melanogaster.' Suzanne L. Rutherford, 1995 - *F Biology UcSD. *F Madueno et al., 1995, Genetics 139(4): 1631--1647 have Cyp-1 proximal to *F nonA and it is distal (see the map I sent). *F Suzanne *F Suzanne Rutherford *F Howard Hughes Medical Institute *F University of Chicago *F 5841 S. Maryland Ave MC1028 *F Chicago, IL 60637 *F Ph : 773-702-4598 *F fax: 773-702-7254 # *U FBrf0090025 *a Calvi *b B. *t 1996.5.7 *T personal communication to FlyBase *u *F Archived. # *U FBrf0090026 *a Rio *b D.C. *t 1996.10.24 *T personal communication to FlyBase *u *F Archived. # *U FBrf0091269 *a Kerridge *b S. *t 1996.11.6 *T personal communication to FlyBase *u *F Date: Wed, 6 Nov 1996 10:56:29 +0100 *F From: 'kerridge' *F Df(2L)tsh8 is a molecular deletion specific to the tsh gene in 40A *F (Fasano et al., 1991). *F Df(2L)305, induced by B. Wakimoto on P{wAR}4-3, deletes tsh and *F four complementation groups (Kerridge, unpublished) on the telomere *F side of tsh; deletion of 39-40. *F Df(2L)TE48-2, from Marie-Laure Samson, deletes the same four complementation *F groups on telomere side of tsh but not the tsh gene itself (Kerridge, *F unpublished). *F COMPLEMENTATION DATA: *F P832','w1118; P{w+tAR ry+t7.2AR=wAR}4-3: localized on tsh DNA walk 3' to *F the transcription unit. Df(2L)305 induced on this chromosome B. Wakimoto. Shows *F position effect on w+; all w+ insertions (10 insertions localized on tsh walk) *F with this effect (so far) located in/ near the tsh gene. Weak tsh allele with *F tsh8 and Df(2L)305. Viable with Df(2L)TE48. *F P805','w1118; P{w+=*}51/CyO: weak tsh allele (halteres droop, wings held *F up), W+ position effect; escapers (18Cy+/45CyO) with Df(2L)tsh8, lethal with *F Df(2L)305 (107 Cy-) viable with Df(2L)TE48. *F P842','y* w*; P{w+mC=lacW}A3-2-66:viable with three deletions *F P897','y* w*; P{w+mC=lacW}l(2)B4-2-121/CyO: strong tsh allele, w+ *F position effect; lethal with tsh8 (225 Cy-), Df(2L)305 (128 Cy-), viable with *F Df(2L)TE48. *F P1370','P{ry+t7.2=PZ}l(2)0431904319 cn1/CyO: strong allele of tsh; lethal *F with tsh8 (60 Cy) and Df(2L)305 (80 Cy) ; viable with Df(2L)TE48. *F P1176','P{ry+t7.2=PZ}l(2)0207402074 cn1/CyO; ry506: lethal with Df(2L)305 *F (116 Cy) & Df(2L)TE48 (118 Cy). Viable with tsh8. *F P1352','P{ry+t7.2=PZ}l(2)0383203832 cn1/CyO; ry506: Lethal with *F Df(2L)TE48 (184 Cy) and Df(2L)305 (64 Cy); viable with tsh8 *F Steve Kerridge. *F LGPD, CNRS Case 907 *F Parc Scientifique de Luminy *F 13288 Marseille cedex 9 *F FRANCE *F Tel: 91 26 96 03 *F Fax: 91 82 06 82 # *U FBrf0091283 *a Klaembt *b C. *t 1996.12.4 *T personal communication to FlyBase *u *F >From cklaemb@novell.biolan.uni-koeln.de Wed Dec 4 10:11:34 1996 *F Df(3R)23D1 093F - 94F (fails to complement pnt alleles) *F Df(3R)5C1 093E-F; 094C-D (complements pnt alleles) *F Both deficiencies were generated by Sebastian Granderath in my lab, by *F reverting the rosy marker in the rC56 P-element. There is so far no paper *F on these lines. *F Dr. Christian Klaembt *F Institut fuer Entwicklungsbiologie *F Universitaet zu Koeln *F Gyrhofstr 17 *F D-50931 Koeln Germany *F Phone xx49 221 470 3130 *F FAX xx49 221 470 5164 # *U FBrf0091291 *a Bellen *b H.J. *t 1997.1.14 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon Jan 13 13:54:59 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 13 Jan 1997 13:54:59 +0000 *F To: tlitt@ligand.neusc.bcm.tmc.edu *F Subject: Help FlyBase - Nrx map *F Cc: eleanor@gen.cam.ac.uk *F Content-Type: X-sun-attachment *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 13 Jan 1997 13:55:24 +0000 *F Content-Length: 2530 *F Hi Troy, *F I am a curator of FlyBase and at present I am working on your paper: *F Baumgartner et al, 1996, Cell 87: 1059--1068 *F in which you list numerous complementation groups located in the Nrx region *F (Fig 4A, pg 1063). *F Do you know if these complementation groups are those already present in *F FlyBase (see attached file) or have you isolated mutations of new *F complementation groups? *F If these complementation groups are new I shall have to rename them *F continuing the 68F and 69A series: *F 68Fa - 68Fg *F 68Fb - 68Fh *F . *F . *F 69Aa - 69Ah *F 69Ab - 69Ai *F The answer to this question is important as you state that l(3)68Ff is *F allelic to Nrx, I do not want to incorrectly merge these two genes if your *F 68Ff is not the same complementation group as the 68Ff listed in FlyBase. *F If you have any problems with this query please let me know as soon as *F possible. *F Regards, *F Eleanor Whitfield *F FlyBase *F Attachment: *F Translation of star codes: *F \*a gene symbol *F \*c cytological location *F \*i synonym *F \*A allele symbol *F Genes present in FlyBase: *F \*a l(3)68Fa *F \*c 68E3--68F6 *F \*i l(3)F11 *F \*i l(3)V4-5 *F \*i l(3)rsg18 *F \*i rsg18: rose-gespleten region interval 18 *F \*A l(3)68Fa1 *F \*i l(3)FII *F \*A l(3)68Fa2 *F \*i l(3)V4-1 *F \*A l(3)68Fa3 *F \*i l(3)V4-5ts *F \*A l(3)68Fa4 *F \*i l(3)VII-5 *F \*a l(3)68Fb *F \*c 68E3--68F6 *F \*i l(3)rsg19 *F \*i rsg19: rose-gespleten region interval 19 *F \*A l(3)68Fb1 *F \*i l(3)49 *F \*a l(3)68Fc *F \*c 68E3--68F6 *F \*i l(3)rsg20 *F \*i rsg20 *F \*i l(3)68Fc: rose-gespleten region interval 20 *F \*A l(3)68Fc1 *F \*i l(3)BIP *F \*i l(3)BIP *F \*A l(3)68Fc2 *F \*i l(3)igh *F \*a l(3)68Fd *F \*c 68E3--68F6 *F \*i l(3)rsg21 *F \*i rsg21: rose-gespleten region interval 21 *F \*A l(3)68Fd1 *F \*i l(3)1B *F \*a l(3)68Fe *F \*c 68F3--68F6 *F \*i l(3)rsg22 *F \*i rsg22: rose-gespleten region interval 22 *F \*A l(3)68Fe1 *F \*i l(3)69 *F \*A l(3)68Fe2 *F \*i l(3)112.226 *F \*A l(3)68Fe3 *F \*i l(3)113 *F \*a l(3)68Ff *F \*c 68F3--68F6 *F \*i l(3)rsg23 *F \*i rsg23: rose-gespleten region interval 23 *F \*A l(3)68Ff1 *F \*i l(3)21-2 *F \*A l(3)68Ff2 *F \*i l(3)112.139 *F \*A l(3)68Ff3 *F \*i l(3)112.143 *F \*A l(3)68Ff4 *F \*i l(3)112.186 *F \*A l(3)68Ff5 *F \*i l(3)112.25 *F \*A l(3)68Ff6 *F \*i l(3)DII *F \*a l(3)69Aa *F \*c 68F3--69A1 *F \*i l(3)rsg24 *F \*i rsg24: rose-gespleten region interval 24 *F \*A l(3)69Aa1 *F \*i l(3)145 *F \*a l(3)69Ab *F \*c 68F3--69A1 *F \*i l(3)rsg25 *F \*i rsg25: rose-gespleten region interval 25 *F \*A l(3)69Ab1 *F \*i l(3)58-2 *F From hbellen@bcm.tmc.edu Mon Jan 13 20:33:38 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 13 Jan 1997 20:33:38 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 14 Jan 1997 04:41:39 +0800 *F To: eleanor@gen.cam.ac.uk *F From: hbellen@bcm.tmc.edu (Hugo Bellen) *F Content-Length: 1191 *F Hi Eleanor, *F Troy passed me your email. None of the mutations present in Flybase were *F available to us, even after repeatedly trying to obtain them. If you know *F where we can find them, I'll be glad to do the complementation tests to *F help clarify which one is which. We named them according to their *F cytological location and the deficiency they fail to complement. Based on *F our data we are almost sure that these new alleles are alleles of *F previously isolated mutants described by Hoogwerf et al., as we found as *F many complementation groups as they did within the respective areas. Since *F these previous mutations are not available anymore (to our knowledge) we *F did not want to name these genes differently. However, I understand that *F we have not shown that 68Fa in Flybase is nrx IV, and therefore that we *F should have chosen another name. Next time I will send you guys a paper *F before publication so there is no screw-up in the nomenclature. Is this *F sufficient information? *F Hugo *F Hugo Bellen, D.V.M., Ph.D. *F Howard Hughes Medical Institute *F Baylor College of Medicine *F One Baylor Plaza - HHMI - Room T 630 *F Houston, TX 77030 *F Tel 713-798-5272 *F Fax 713-798-8515 *F email hbellen@bcm.tmc.edu # *U FBrf0091325 *a Miklos *b G.L.G. *t 1997.1.29 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Mon Jan 20 14:29:26 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 20 Jan 1997 14:29:26 +0000 *F To: miklos@nsi.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Mon, 20 Jan 1997 14:31:43 +0000 *F Content-Length: 927 *F Dear Dr. Miklos, *F I am a curator working for FlyBase. I am currently curating a paper of yours : *F J. Neurogenetics 6: 133-151 (1990). (Miklos and de Couet). *F In the complementation map in Figure 1 (page 136) there is a gene symbol *F 'soz' (between fog and stn). I would be grateful if you could tell me the *F full name of this gene, and any other information about it such as whether *F it is known by any other synonym, as we do not have any information about *F this gene in FlyBase. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From miklos@nsi.edu Wed Jan 29 02:03:09 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 29 Jan 1997 02:03:09 +0000 *F Date: Tue, 28 Jan 1997 18:05:10 -0800 (PST) *F X-Sender: glgm@popmail *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Gillian Millburn (Genetics) *F From: miklos@nsi.edu (George L. Gabor Miklos) *F Subject: Re: FlyBase query sozzled soz *F Content-Length: 586 *F Dear Ms Millburn, *F The soz notation was given to the EA41 lethal complementation group which *F lies between fog and stoned ( Perrimon et al 1990; Genetics, 121,313-331). *F We called it sozzled because the escapers were unsteady on their feet. *F I hope this has not caused too much of a trauma. *F Please let me know how you wish to proceed. *F yours sincerely *F george.L.gabor miklos *F George L. Gabor Miklos | *F The Neurosciences Institute | *F 10640 John Jay Hopkins Drive | e-mail: miklos@nsi.edu *F San Diego, CA 92121 | Fax: 619-626-2199 # *U FBrf0091335 *a Berg *b C. *t 1996.12.4 *T personal communication to FlyBase *u *F From berg@mendel.genetics.washington.edu Wed Dec 04 03:00:14 1996 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 4 Dec 1996 03:00:14 +0000 *F Date: Tue, 3 Dec 1996 20:02:45 -0800 *F From: 'Celeste Berg' *F Reply-To: 'Celeste Berg' *F To: ma11@gen.cam.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Subject: Re: Help Michael *F Content-Length: 2329 *F Hi Michael (and Rachel): *F Please give my apologies to Rachel, who twice emailed me about this. I don't *F know how much information you need; the perfectionist that I am assumed I needed *F to submit the sequence to a database for it to be acceptable to you. *F At any rate, Doreen Gillespie and Todd Enderlein in my lab have sequenced the *F genomic DNA upstream from homeless and have identified an ORF with strong *F homology to the 23 kD subunit of bovine NADH dehydrogenase complex I. Over a *F 174 amino acid stretch there is 83% identity and 93% similarity and includes a *F core motif CysXXCysXXCysXXXCysPro known to bind iron through a sulfur linkage in *F ferrodoxins. The bovine gene is called 'NADH dehydrogenase' (ubiquinone) (EC *F 1.6.5.3). We assume this ORF is the Drosophila homolog of the mitochondrial *F enzyme. We have not sequenced the cDNA (we have had trouble subcloning the *F phage insert into Bluescript and the gene may be lethal in high copy in E. *F coli). The genomic DNA has at least one intron that interrupts the core motif. *F I feel confident saying this homology is significant enough to state the gene *F should be named 'NADH dehydrogenase'. *F If we need to submit the sequence to Genbank, I won't be able to get to it until *F Christmas or January even. *F Regards, *F Celeste *F In message writes: *F > Celeste - *F > *F > Rachel sent this recently. Can you give us any help ? *F > Thanks *F > *F > Michael Ashburner. *F > *F > Dear Celeste, *F > *F > I am writing about a gene you reported in: *F > \*x FBrf0083956 == Gillespie and Berg, 1995, Genes Dev. 9(20): 2495--2508 *F > *F > The gene in question is the gene adjacent to fs(3)hls that is related to *F > bovine NADH dehydrogenase. We are in the process of drawing new gene maps *F > of the genome, and for this all transcription units need to have a gene *F > name. Anonymous transcription units get symbols of the format 'anon-xyz' *F > but it seems a shame to confer such a symbol on a transcription unit you *F > know so much about. We have not been able to find this NADH *F > dehydrogenase gene in the sequence databases. Is anyone working on or about *F > to publish on it? Do they have a name/symbol for it? *F > *F > If not, please could you make a suggestion as to what you would like the *F > symbol to be. *F > *F > Many thanks, *F > *F > with best wishes, *F > *F > Rachel. # *U FBrf0091336 *a Roote *b J. *t 1996.12.3 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Tue Dec 03 16:50:45 1996 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 3 Dec 1996 16:50:45 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 3 Dec 1996 16:51:35 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Content-Length: 373 *F Aubrey, *F I have further mapped 2 of the Spradling collection. We have EMS alleles *F of both. *F PZ01289 *F nap4 18/50 *F Drlrv17 0/71 *F nap12 10/47 *F Drlrv3 0/154 *F Drlrv28 0/130 *F spleN3 45/119 *F pk78k 62/169 *F PZ04524 59/237 *F Dfpk-sple22 62/126 *F PZ04524 *F Drlrv3 0/170 *F nap2 0/128 *F Cheers, *F John *F From jr32@mole.bio.cam.ac.uk Wed Dec 04 17:35:17 1996 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 4 Dec 1996 17:35:17 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 4 Dec 1996 17:36:02 +0000 *F To: rd120@gen.cam.ac.uk *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: pc *F Content-Length: 739 *F >I am curating this, right now! What do you want to call the EMS alleles, *F >l(2)012891 and l(2)045241? *F > *F >Rach *F Well I'm being a little coy! I had named the genes l(2)42Ea and l(2)42Ed *F resp. for the benefit of myself and others working on genes in this region, *F but Ea is at odds with the insitu for 1289 (42C8-9) and the cytology of *F some of the dfs that define this gene is a bit dodgy. 42D is probably *F closer. *F Maybe until (if ever) our genetics is published they should be called *F l(2)01289 and l(2)04524 as you suggest? *F The EMS alleles that we have of 1289 are: *F 2A2 *F 2D10 *F 2F2 *F 2C4 *F 2B4 *F 1BH *F 1BI *F 1DG *F 483 (from Segal) *F 485 ( ' ) *F GJ63/7 *F and 04524: *F 1A4 *F 1D17 *F 1BA *F 1DL *F GJ63/9 *F ..so we could just number these 2 to 11, etc. *F J # *U FBrf0091337 *a Leevers *b S. *t 1996.12.5 *T personal communication to FlyBase *u *F From sallyl@ludwig.ucl.ac.uk Tue Dec 03 17:15:38 1996 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 3 Dec 1996 17:15:38 +0000 *F X-Sender: sallyl@kestrel.ludwig.ucl.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 3 Dec 1996 17:18:08 +0100 *F To: rd120@gen.cam.ac.uk *F From: sallyl@ludwig.ucl.ac.uk (Sally Leevers) *F Subject: Drosophila Dp110 *F Content-Length: 850 *F Dear Rachel, *F Just to update you on the Drosophila PI3K originally called PI3K-92D. We *F now have a paper in press in EMBO Journal describing this Drosophila PI3K *F which we have renamed Dp110 (to be consistent with the nomenclature for *F these enzymes in other organisms) and the phenotypes generated by *F over-expression of activated and dominant negative forms of the protein. *F Also, we have remapped the gene to 92E12-13 (making the original name even *F less appropriate!). *F The sequence should be released into EMBL shortly, under accession number *F Y09070. *F Please let me know if there is any other information that might be of use *F to you. *F with best wishes, *F Sally *F Sally J. Leevers *F Ludwig Institute for Cancer Research *F University College London Branch *F 91 Riding House St *F London W1P 8BT *F England *F Tel/Voice mail: 0171 878 4067 *F Fax: 0171 878 4040 # *U FBrf0091341 *a Kaufman *b T. *t 1997.1.2 *T personal communication to FlyBase *u *F From kaufman@sunflower.bio.indiana.edu Thu Jan 02 14:01:21 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 2 Jan 1997 14:01:21 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 2 Jan 1997 09:08:05 -0500 *F To: Rachel Drysdale (Genetics) *F From: kaufman@sunflower.bio.indiana.edu (T. Kaufman) *F Subject: Re: genetic variants *F Content-Length: 653 *F >Re: *F >*x FBrf0082042 == Gorman and Kaufman, 1995, Genetics 140(2): 557--572 *F >do you know which TM3 it is in TM3-lacZ-hg? *F The TM3 version into which this lac Z reporter landed is the one marked *F with Sb but not Ser. The recessive markers are those in the original TM3 *F except the y+ was recombined off. Is that what you wanted to know Rach? *F \--tk *F Thom Kaufman kaufman@sunflower.bio.indiana.edu *F \--- Biology Dept., Indiana University, Bloomington, IN 47405 --- *F 812-855-3033/Office--812-855-7674/Lab--812-855-2577/FAX # *U FBrf0091342 *a Prokopenko *b S. *c H.J. *d Bellen *t 1997.1.7 *T personal communication to FlyBase *u *F >From sp691305@imgen.bcm.tmc.edu Mon Jan 06 16:23:20 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Mon, 6 Jan 1997 16:23:20 +0000 *F From: sp691305@imgen.bcm.tmc.edu (Sergei Prokopenko) *F Subject: updates for FlyBase *F To: ag24@gen.cam.ac.uk *F Date: Mon, 6 Jan 1997 10:24:19 -0600 (CST) *F Reply-to: sp691305@imgen.bcm.tmc.edu *F Organization: Molecular and Human Genetics, Baylor College of Medicine *F Phone: (713)798-5273 *F x-fax-number: (713)798-8515 *F x-phone: (713)798-8841 *F X-Mailer: ELM version 2.4 PL23 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=US-ASCII *F Content-Transfer-Encoding: 7bit *F Content-Length: 2958 *F Dear Dr. de Grey, *F If you remember, we communicated some time ago about bunched and quo vadis. *F Some of the mutations identified by us on the second chromosome (Genetics *F 139: 1663-1678) turned out to be alleles of previously cloned and *F characterized genes. Therefore, Hugo has asked me to send you this *F information, so that the appropriate gene reports in FlyBase could be *F updated. *F 1. anarchist (anch) (allele 78/5) is allelic to three rows (thr) *F (Nusslein-Volhard et al., 1984). *F I have plasmid rescued the genomic fragments flanking the 78/5 insertion *F on both sides (using EcoRI and BamHI restriction enzymes) and sequenced *F their ends. The sequences of the proximal ends of both genomic rescues *F show the 100% match (over 454 and 196 nt.) to the published sequence of *F the thr cDNA (Philp et al., 1993; D'Andrea et al., 1993). *F In addition, the 78/5 allele of anch failed to complement thr1 allele *F (0/129). *F Finally, the 78/5 insertion (anch) was mapped by in situ to 54F (Kania et *F al., 1995) and thr maps at 54F3-6. *F 2. fondue (fond) (alleles 25/14 and 50/2) is allelic to cyclin E (cycE). *F I have plasmid rescued the genomic fragments flanking the 25/14 insertion *F on both sides. The distal end of the 7.0 kb BamHI rescue showed a 90% *F match (over 80 nt.) to the published sequence of the type II cyclin E mRNA *F (Richardson et al., 1993; Knoblich et al., 1994). This suggested that the *F 25/14 P element is inserted in the second intron of the type II cycE mRNA. *F In addition, a complementation test between the 25/14 allele of fond and *F CycE05206 allele indicated that the two mutations are allelic. Flies *F transheterozygous for the two mutations (60/169) are viable, but have rough *F eyes and wing venation defects. *F Finally, cyclin E maps at 35D5-7 and the two alleles of fondue (25/14 and *F 50/2) were mapped by BDGP to 35D3-4 and 35D1-2; 44D5-6, respectively. *F 3. Similarly, I have an independent molecular proof that the 81/4 *F insertion affects escargot, and that floater (allele 69/21) is allelic to *F Star. However, this has been already reported to FlyBase by the BDGP based *F on the results of genetic analyses. *F This information, if included in FlyBase, should be refered to as *F S.Prokopenko and H.J.Bellen, personal communication. *F Fell free to contact either myself or Hugo, if you have any questions or *F would like me to send more detailed information. *F Sincerely, *F Sergei Prokopenko # *U FBrf0091343 *a Carpenter *b A.T.C. *t 1997.1.10 *T personal communication to FlyBase *u *F From atc12@mole.bio.cam.ac.uk Fri Jan 10 14:05:08 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 10 Jan 1997 14:05:08 +0000 *F Date: Fri, 10 Jan 1997 14:07:01 +0000 (GMT) *F From: Adelaide T C Carpenter *F Reply-To: Adelaide T C Carpenter *F To: Rachel Drysdale , Aubrey deGrey *F Subject: Shilo's LG9 mutant *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 682 *F is dead as a doornail over Df(3L)fz-GS1a and over fz-GF3b (confirming *F Shilo) as well as over Df(3L)D-1rv16; completely viable over l(3)70Da1 *F and over dev1; completely viable and female-fertile over Df(3L)fz-M21; *F and completely lethal over D3 (loD3) and D7 (loD7). It is *F therefore an allele of loD (presumably to be called, for the nonce, *F loDLG9). I haven't completely finished the counts but I just did the *F last count on the first vial, the numbers are large and the conclusion is *F solid. I haven't yet done cytology so can't eliminate the possibility *F that it's a very small deletion, but the genetics certainly suggest *F nothing of the kind. *F Cheers, Adelaide # *U FBrf0091344 *a Bloomington Drosophila Stock Center *b ?. *t 1997.1.16 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Assorted alleles and aberrations at Bloomington that are not in FlyBase *F Date: 15 January 1997 *F *F Information communicated: *F The following aberrations or alleles that appear in the Bloomington Stock List are not in FlyBase. The aberration or allele symbol is followed by *F the stock record, followed by any additional relevant information provided by the donor. *F *F Df(3R)Dr-rv1 *F *F #669 w[*]; Df(3R)Dr-rv1, ry[506]/TM3, Sb[1] ry[RK] *F Breakpoints: 099A01-02;099B06-11 *F Donor: Adelaide Carpenter *F Comments: Progenitor carried P{w[+mC] sox[hs]}, w[+mC] still there, but don't know about the rest, A.T.C.C. *F Additional info: P\T-derived from ry[506] Dr[1] P{w[+mC] sox[hs]}F-13 P{Delta2-3}99B (valid symbols for sox allele and transposon construct unavailable) *F *F P{w[+mC]=lacW}M(3)66D[1] *F *F #2247 y[*] w[*]; P{w[+mC]=lacW}M(3)66D[1]/TM2 *F Donor: Andrew Lambertsson *F Comments: strong to extreme Minute, encodes ribosomal protein RPL14, A.L. *F Additional info: S. Saebow-Larssen, A. Lambertsson, in preparation. *F *F stil[3] *F *F #533 stil[3]/CyO *F Donor: Pamela Mulligan *F Comments: EMS-induced allele, Dev. Genet. 18: 316-326 (1996), P.M. *F *F T(2;3)CyO-TM6B *F *F #602 P{w[+t10.5]>=X6}Su(H)[M28]/T(2;3)CyO-TM6B, Tb[1] *F Donor: Michael Ashburner Donor's source: Claudio Sunkel *F Comments: originally from J.-M. Dura, Bloomington stock P1356, removed as uncharacterized, K.M. 8/19/96 # *U FBrf0091346 *a Kelly *b L. *t 1997.1.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Dec 11 15:12:26 1996 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 11 Dec 1996 15:12:26 +0000 *F To: len_kelly.genetics@mac.unimelb.edu.au *F Subject: helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F From: Rachel Drysdale (Genetics) *F Date: Wed, 11 Dec 1996 15:10:36 +0000 *F Content-Length: 3310 *F Dear Dr. Kelly, *F I am doing some work on your 'stoned is a dicistronic transcript' paper *F \*x FBrf0089585 == Andrews et al., 1996, Genetics 143(4): 1699--1711. *F We originally curated the data as though it all belonged under the *F 'stoned' gene, stn. But the biology of the situation dictates that *F we split the data into two new 'genes', stnA and stnB. To do *F this I must partition the alleles of the defunct 'stn' gene into the *F new stnA and stnB and this is no easy task. Here are the alleles we *F currently have for stn (with the \*a representing the valid symbol and *F the \*i lines synonyms), with an arrow denoting which of the two the *F allele should become, where I have been able to figure it out. *F \*A stn1 *F \*i l(1)8P1 *F \*i 8P1 *F \*i stn8P1 *F \*A stn10 *F \*i l(1)VA228 *F \*A stn11 *F \*i l(1)VE720 *F \*i stnVE720 *F \*A stn12 --> stnA *F \*i l(1)13-120 *F \*i stn13-120 *F \*A stn14 --> stnB *F \*i l(1)PH1 *F \*i stnPh1 *F \*i stnPH1 *F \*A stn15 --> stnA *F \*i stnC *F \*i stnC *F \*A stn2 *F \*i l(1)M143 *F \*A stn3 --> stnA and stnB double mutant phenotype, - polarity *F \*i l(1)R-9-10 or double mutant? *F \*i R-9-10 *F \*A stn4 *F \*i l(1)R-9-15 *F \*i R-9-15 *F \*A stn5 *F \*i l(1)X3 *F \*i stnX3 *F \*i X3 *F \*i X-3 *F \*A stn6 --> stnB *F \*i stnts1 *F \*A stn7 --> stnB *F \*i stnts2 *F \*A stn8 *F \*i l(1)C88 *F \*A stn9 *F \*i l(1)HC121 *F \*A stnR-1-10 *F \*A stnS64 *F \*A stnVE814 *F I have also looked at *F \*x FBrf0020833 == Lifschytz and Falk, 1969, Mutat. Res. 8: 147--155 *F and *F \*x FBrf0058577 == Petrovich et al., 1993, Genetics 133(4): 955--965 *F I notice that there is no chromosomal orientation for your Figure 1B. *F If there were I might (optimistically, perhaps) assign stn5 and stn4, *F since Lifschytz clearly distinguishes them (X3 and R-9-15 in his Figure 2). *F Perhaps you know the chromosomal orientation by now? *F Another problem is that, since these two proteins are encoded from the *F same transcript, the complementation data will never be simple (polarity *F effects etc), as I discovered from the Petrovich paper. The only firm *F 'yes/no' conclusion that I could draw from this paper was that the stn ts *F alleles fall into a different group from stn15 (stnC), which is backed *F up by your molecular findings. *F Do you have any molecular information that would allow me to assign stnA/stnB *F status to the remaining alleles stn1, stn10, stn11, stn2, stn4, *F stn5, stn8, stn9, stnR-1-10, stnS64 and stnVE814. Your paper *F suggests that there is nothing to be found (molecularly) for stn6 (ts1), *F stn7 (ts2), stn15 (C), stnS64 or stn1 (8P1). *F For any that must remain unassigned I will simply place them in stnA with a *F note saying that the allele may actually represent a lesion in stnB, and move *F them as/when the data becomes available. *F Any light you can shed on the sitation, including your opinion of my *F assignments to stnA and stnB above, would be most welcome, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From Len_Kelly@muwayf.unimelb.edu.au Wed Jan 22 22:15:07 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 22 Jan 1997 22:15:07 +0000 *F Date: Thu, 23 Jan 1997 09:15:05 +1100 *F From: Len Kelly *F Subject: Re: helping FlyBase *F To: Genetics *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-transfer-encoding: 7BIT *F Content-Length: 1306 *F Reply to: RE>helping FlyBase *F Dear Rachel, *F I am sorry I have been tardy in replying to your e-mail, *F but I have been overseas, and I have also been loathed to place data in 'fly *F base' without further experimental data confirming the location of the various *F stoned alleles. We now have MOLECULAR data SUGGESTING that both stnC and the *F stnts1 and stnts2 alleles MAY be within the stnA 'gene'. In fact the only *F stoned allele that we can now unambiguously place in the stnB gene is the *F stnPH1 allele, which is an insertion of an I factor in the second ORF. We *F really would like to obtain harder molecular data on the stnC and stnts *F alleles, as these alleles are central to an understanding of the *F complementation data in the Genetics paper (Andrews et al., 1996). *F At least temporarily, I would place all the behavioral and lethal alleles of *F stoned into stnA with the exception of stnPH1 which is certainly in stnB, but *F which may be having effects on the expression from the stnA cistron. *F I'm sorry I can't be of more help, but our data at the moment just doesn't *F allow for a complete clarification of the situation. If you can think of any *F other ways in which I might be able to help, please let me know. *F Again I'm sorry that I didn't get back to you sooner. *F Regards, *F Len Kelly # *U FBrf0091347 *a Roote *b J. *t 1997.2.3 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Mon Feb 03 16:59:09 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Mon, 3 Feb 1997 16:59:09 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 3 Feb 1997 17:01:23 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Content-Length: 191 *F Aubrey, *F Eine kleine new datum for In(2LR)b71k1A: *F Formalized genetic data l(2)34Db << bk1 << Sos << b << bk2 << l(2)34Dc << *F bk3 hits l(2)43Bc *F (i.e. we know where the 2R break is) *F John # *U FBrf0091348 *a Shestopal *b S.A. *t 1997.2.6 *T personal communication to FlyBase *u *F Archived. # *U FBrf0091349 *a Peifer *b M. *t 1997.2.10 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Mark Peifer, University of North Carolina *F To: Bloomington Drosophila Stock Center *F Subject: P{lArB}A251.1F3 is a puc allele *F Dated: 4 Feb 1997 *F *F Information communicated: *F Complementation tests show P{lArB}A251.1F3 to be an allele of puc. # *U FBrf0091478 *a Burtis *b K.C. *t 1996.2.19 *T personal communication to FlyBase *u *F >From kcburtis@ucdavis.edu Tue Feb 18 21:38:50 1997 *F X-Sender: fzburtis@boris.ucdavis.edu *F Mime-Version: 1.0 *F Date: Tue, 18 Feb 1997 18:37:29 -0800 *F To: crosby@morgan.harvard.edu (Madeline Crosby) *F From: kcburtis@ucdavis.edu (Ken Burtis) *F Subject: Re: pAct5CSRS *F *F Hi Lynn, *F *F It took a while, but I found my notebook entries on the construction of *F pACT5CSRS (March 1987). I'm not even sure how you tracked me down since *F the Han et al paper referenced K. Burgess. *F *F The genesis of the vector was as follows: *F *F The pUC18 vector was modified to remove the EcoRI site by end filling, so *F the new polylinker sequence around teh Ri site would be "GAATTAATTC". *F *F The 5' fragment of Act5C was obtained by digesting pUChsneo Act5C (obtained *F from Howard Lipshitz) by Bgl II + BamH I, and cloning the ~2.6 kb fragment *F obtained into the BamH I site of Larry Marsh's vector pIC20R (Reference: *F Gene 32:481-485 (1984)). The orientation of this clone was such that the 5' *F end of this fragment (relative to the direction of Act5C transcription) was *F adjacent to the Sal I site in the pIC20R vector, and the 3' end adjacent to *F the EcoRI site. The resulting subclone was then digested by Sal I and *F EcoRI to obtain an ~ 2.6 kb Sal-RI fragment. *F *F *F The 3' fragment of Act5C was obtained by digesting pUChsneo Act5C (obtained *F from Howard Lipshitz) by Sal I, and cloning the ~1.1 kb fragment into *F pIC20R digested with Sal I and Xho I. The orientation of this clone was *F such that the 3' end of this fragment (relative to the direction of Act5C *F transcription) remained a Sal I site. This subclone was then digested with *F EcoRI plus Sal I to obtain an ~ 1.1 kb RI-Sal fragment. *F *F These two fragments were mixed and ligated and then digested with Sal I. *F The fusion fragment (Sal-5'Act5C-RI-3'Act5C-Sal) was then cloned into the *F modified pUC18 vector with filled-in EcoRI site. The orientation of the *F insert relative to the pUC18 polylinker is *F HIII-PstI-SalI-5'Act5C-3'Act5C-SalI-XbaI-BamHI-SacI-EcoRI(deleted). *F *F This created the pACT5CSRS vector, which I would estimate to be about 6.4 *F kb (not 10363 bp as listed in the Flybase entry). *F *F Also, the report on the vector in the link on the transposon search page *F contains one incorrect reference to a paper from Holmgren's lab - this *F involved a completely different Act 5C vector. *F *F If you'ld like, you could list K. Burtis, personal communication as a *F reference since I never published the vector. *F *F Hope this was helpful. *F *F Best regards, *F *F Ken *F *F *F *F *F *F *F >Hi, Ken, *F > *F >FlyBase is compiling data and maps on some of the more useful *F >P transformation and cell culture vectors. We will be publishing *F >them very shortly in DIS. pAct5CSRS is on the list, and I would *F >appreciate some input from you if you can fill in some holes and/or *F >verify that what I've been able to piece together so far is correct. *F > *F >I've put together an annotated map and a fairly complete sequence *F >based on info in Han et al., 1989. You can access them through *F >TransposonSearch (at the top of the page is a "New - annotated maps" *F >button). If you would prefer, I can e-mail the sequence to you. *F > *F >If you spot any errors in any of the other vectors in the annotated *F >maps list, that information would also be most appreciated. *F > *F >Hope to hear from you soon, *F > *F > *F >--Lynn *F > *F > *F >Lynn Crosby *F >FlyBase *F *F *F *F *F ----- End Included Message ----- *F *F # *U FBrf0091566 *a Fokta *b F.J. *t 1997.3.8 *T personal communication to FlyBase *u *F ----- Begin Included Message ----- *F *F >From 5043foktaf@vms.csd.mu.edu Tue Oct 29 16:26:33 1996 *F Date: Tue, 29 Oct 1996 15:23:59 -0600 *F From: "Frank J. Fokta IV" <5043foktaf@vms.csd.mu.edu> *F Subject: *F To: 'Lynn Crosby' *F Mime-Version: 1.0 *F Content-Type: text/plain; charset="us-ascii" *F Content-Transfer-Encoding: quoted-printable *F Content-Length: 284 *F *F Dear Lynn, *F *F I just received the map of the pTURBO helper plasmid. Surprise! It's *F pUchsDelta2-3. Maybe this deserves mention in the alias list for *F delta2-3. Have you gotten any info on the size discrepancy between the *F 7.3KB on my map and the 8+ KB on your compilation? *F *F Frank *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From 5043foktaf@vms.csd.mu.edu Fri Nov 1 13:11:36 1996 *F Date: Fri, 01 Nov 1996 12:09:53 -0600 *F From: "Frank J. Fokta IV" <5043foktaf@vms.csd.mu.edu> *F Subject: *F To: 'Lynn Crosby' *F Mime-Version: 1.0 *F Content-Type: text/plain; charset="us-ascii" *F Content-Transfer-Encoding: quoted-printable *F Content-Length: 881 *F *F Dear Lynn, *F *F The following is a table of predicted fragment sizes from your *F compilation, and the actual sizes of fragments that I obtained from a *F given restriction enzyme. Please note that these are really crude *F estimates for my diagnostic purposes. See if you can make any sense *F out of these. If you need more accurate numbers, I can re-perform *F these experiments with the same or different enzymes if you see *F fit. Note also, that in estimating these fragment sizes, I assumed *F that the plasmid was 7.3 Kb which was reasonable on the gel system I *F used. Let me know if I can be of further assistance. *F *F Frank *F *F Predicted (bp) Actual (Kb) *F *F BamHI *F 5392 4.8 *F 2686 2.5 *F *F Eco RI *F 5838 4.8 *F 2240 2.5 *F *F HindIII *F 5709 3.5 *F 1531 3.0 *F 830 0.8 *F *F SalI* *F 4384 5.5 *F 3676 1.8 *F 18 *F *F XbaI *F 6916 7.3 *F 1162 *F *F * The third fragment (18bp) would have been missed on the gel system I used. *F *F ----- End Included Message ----- *F *F ----- Begin Included Message ----- *F *F From: Madeline Crosby[SMTP:crosby@morgan.harvard.edu] *F Sent: Friday, November 01, 1996 8:25 AM *F To: 5043foktaf@vms.csd.mu.edu *F Cc: crosby@morgan.harvard.edu *F Subject: Re: pUChsDelta2-3 *F *F Frank, *F *F Thanks for the info. Looks more screwed up than I had realized. *F One quick question: did you run the BamHI and EcoRI cuts on the same *F gel? In other words, do we know for sure that both cuts produce the *F same size fragments (whatever the actual size of those fragments *F may be)? *F *F I assume that you have used this thing and know that it works as *F advertised... *F *F --Lynn *F *F ----- End Included Message ----- *F *F ----- Begin Included Message ----- *F *F >From 5043foktaf@vms.csd.mu.edu Fri Nov 1 15:00:14 1996 *F Date: Fri, 01 Nov 1996 13:56:17 -0600 *F From: "Frank J. Fokta IV" <5043foktaf@vms.csd.mu.edu> *F Subject: RE: pUChsDelta2-3 *F To: 'Madeline Crosby' *F Mime-Version: 1.0 *F Content-Type: text/plain; charset="us-ascii" *F Content-Transfer-Encoding: quoted-printable *F Content-Length: 905 *F *F The BamHI and EcoRI digests were run on the same gel in adjacent *F lanes. Being that they were exactly the same size fragments, these *F would be candidate enzymes to repeat in the event that you can't make *F sense of these fragments. The plasmid described here has bestowed many *F hardy transformants upon me. Keep in touch. *F *F Frank *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F >From 5043foktaf!@vms.csd.mu.edu Thu Mar 6 10:58:25 1997 *F Date: Thu, 06 Mar 1997 10:03:37 -0600 *F From: "Frank J. Fokta IV" <5043foktaf!@vms.csd.mu.edu> *F Subject: puchspidelta2-3 *F To: 'Lynn Crosby' *F Mime-Version: 1.0 *F Content-Transfer-Encoding: quoted-printable *F Content-Type: text/plain; charset="us-ascii" *F Content-Length: 1499 *F *F Hi Lynn, *F *F I was able to do some more digests of the helper plasmid. The *F following is the list of fragment sizes (actual) vs expected from the *F compiled sequence: *F *F Eco RV *F Expected Actual *F 8078 7400 *F *F Hind III *F Expected Actual *F 5709 3450 *F 1531 3000 *F 838 1000 *F *F Pst I *F Expected Actual *F 3890 4950 *F 1511 1300 *F 1212 900 *F 797 760 *F 668 *F *F Sst I *F Expected Actual *F 5283 5375 *F 2795 2175 *F *F Xba I *F Expected Actual *F 6916 7400 *F 1162 *F *F Xho I *F Expected Actual *F 7103 7400 *F 975 *F *F Bam HI *F Expected Actual *F 5392 4750 *F 2686 2600 *F *F Eco RI *F Expected Actual *F 5838 4700 *F 2240 2600 *F *F Sal I *F Expected Actual *F 4384 5375 *F 3676 1700 *F 18 *F *F Pvu II *F Expected Actual *F 2364 2400 *F 1856 1900 *F 1595 1000 *F 896 800 *F 794 *one of the smaller must be a doublet *F 573 *F *F These sizes are approximations based on the results from DNA mobility *F analysis software, but by "eye", they look reasonable. Since you *F compiled the sequence, perhaps these numbers can give you an idea *F where the problem resides. I know time is short at this point, *F however, if you can find the problem with the sequence (or Map), and *F you need additional digests, or double digests etc., to verify the new *F compilation, let me know. *F *F Frank *F Frank J. Fokta IV *F Department of Biology *F Marquette University *F Milwaukee, WI *F 5043FoktaF@vms.csd.mu.edu *F *F ----- End Included Message ----- *F *F *F ----- Begin Included Message ----- *F *F From: Madeline Crosby[SMTP:crosby@morgan.harvard.edu] *F Sent: Thursday, March 06, 1997 2:07 PM *F To: 5043foktaf!@vms.csd.mu.edu *F Cc: crosby@morgan.harvard.edu *F Subject: Re: puchspidelta2-3 *F *F *F Frank, *F *F I've played around and get a good fit for your restriction data if I: *F *F 1) Reverse the orientation of the vector segment (thus reversing the *F restriction sites at either end of it). *F *F 2) Replace the pBR322-Hsp70 fragment with 800 n's (no sequence, *F 800bp long). *F *F I did number 2 because none of the sites that should be in this *F segment appear to be there: SalI, XhoI, XbaI, PstI. I predict that *F there IS a PvuII site. It really looks like this fragment is NOT *F what it is advertised to be. But.. it works...so what does that *F mean ??!!! *F *F May I cite our correspondence as a personal communication *F to FlyBase? I would like to attach some brief cautionary explanation *F to the map. *F *F *F --Lynn *F *F ----- End Included Message ----- *F *F ----- Begin Included Message ----- *F *F *F >From 5043foktaf@vms.csd.mu.edu Fri Mar 7 16:26:57 1997 *F Date: Fri, 07 Mar 1997 15:22:03 -0600 *F From: Frank J Fokta IV <5043foktaf@vms.csd.mu.edu> *F Subject: RE: puchspidelta2-3 *F To: 'Madeline Crosby' *F Mime-Version: 1.0 *F Content-Type: text/plain; charset="us-ascii" *F Content-Transfer-Encoding: quoted-printable *F Content-Length: 1462 *F *F Hi Lynn, *F *F I have to agree...something is screwed up. I'm going to test another *F source to see if the plasmid is the same (the third one!). I will also *F try a couple of double digests this weekend. Maybe this will shed some *F light on the situation. ... Yes, you may cite our correspondence. I'll *F keep you updated. *F *F Thanks, *F *F Frank *F *F ----- End Included Message ----- *F # *U FBrf0091567 *a Brand *b A.H. *t 1997.3.8 *T personal communication to FlyBase *u *F >From ahb@mole.bio.cam.ac.uk Fri Mar 7 14:30:36 1997 *F Mime-Version: 1.0 *F To: crosby@morgan.harvard.edu (Madeline Crosby) *F From: ahb@mole.bio.cam.ac.uk (Andrea Brand) *F Subject: Re: GAL4/UAS constructs *F Date: Fri, 7 Mar 1997 19:30:11 +0000 *F *F >sequence for the 5xUAS portion of pP{UAST}: *F *F BamHI site to XbaI site or so (includes 5xGAL4 binding sites, promoter, *F polylinker. *F *F GGATCCAAGCTTGCATGCCTGCAGGTCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCC *F GAGCGGAGTACTGTCCTCCGAGCGGAGTACTGTCCTCCGAGCGGAGACTCTAGCGAGCGCCGGAGTATAAATAGAGGCGC *F TTCGTCTACGGAGCGACAATTCAATTCAAACAAGCAAAGTGAACACGTCGCTAAGCGAAAGCTAAGCAAATAAACAAGCG *F CAGCTGAACAAGCTAAACAATCTGCAGTAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAGCAACCAAGTAAATC *F AACTGCAACTACTGAAATCTGCCAAGAAGTAATTATTGAATACAAGAAGAGAACTCTGAATAGGGAATTGGGAATTCGTT *F AACAGATCTtGCGGCCGCGCTCGAGGGTACCTCTAGAGG # *U FBrf0091568 *a Newfeld *b S. *t 1997.3.15 *T personal communication to FlyBase *u *F Archived # *U FBrf0091569 *a Freeman *b M. *t 1997.3.15 *T personal communication to FlyBase *u *F lastname : Freeman *F givenname : Matthew *F institution : MRC Laboratory of Molecular Biology *F email : mf1@mrc-lmb.cam.ac.uk *F author1 : M. Freeman *F year : 1996 *F journal : Cell *F volume : In press *F FBgu ID : FBgu0000002=20 *F date_updated: *F Sep 23 1996 7:47:44:176AM *F transposon: *F P(gal4-GMR) - Glass Multimer Reporter *F vector: *F pP(CaSpeR-hs) *F synthetic: *F 5 copies of glass binding site from Rh1 enhancer. *F Corresponds to construct 29-2 in Ellis et al. *F Development (1993) 119, 855 *F vector_comments: *F Has some hsp70 promoter sequences: toxic UAS constructs can usually *F be grown at 18=B0. *F visualization: *F UAS-lacZ *F expression: *F Expressed very strongly in all cells posterior to=20 *F morphogenetic furrow. Other expression not examined. *F insertion_symbol: *F P(GMR-Gal4) \#12 *F chromosome: *F 2 *F insertion_property: *F viable *F insertion_comments: *F This was the weakest insertion obtained. Most others had rough eyes *F presumably due to toxicity of massively overexpressed Gal4. This one is *F wild type as a heterozygote at 25oC and lower. As a homozygote, and at *F higher temperatures, individuals exhibit non-specific eye defects. *F date_updated: *F Sep 23 1996 7:47:44:176AM # *U FBrf0091570 *a Johnson *b R.L. *t 1997.3.15 *T personal communication to FlyBase *u *F lastname : Johnson *F givenname : Ron *F institution : Department of Developmental Biology, Stanford University *F email : rjohnson@cmgm.stanford.edu *F author1 : R L Johnson *F author2 : et al. *F year : 1995 *F journal : Development *F volume : 121 *F start_page : 4161 *F end_page : 4170 *F FBgu ID : FBgu0000019 *F date_updated: *F Oct 4 1996 1:55:17:983PM *F transposon: *F P(UAS-ptc) *F vector: *F pUAST-ptc *F cDNA: *F patched *F dbid: *F M28418 *F insert_size: *F 4 kb *F insertion_symbol: *F P(UAS-ptcB1) *F chromosome: *F 3 *F insertion_property: *F viable *F insertion_symbol: *F P(UAS-ptcB2) *F chromosome: *F 3 *F insertion_property: *F viable *F insertion_symbol: *F P(UAS-ptcB3) *F chromosome: *F 2 *F insertion_property: *F viable *F insertion_symbol: *F P(UAS-ptcC) *F chromosome: *F 1 *F insertion_property: *F viable # *U FBrf0091571 *a Kaneko *b M. *t 1997.3.14 *T personal communication to FlyBase *u *F lastname : Kaneko *F givenname : Maki *F institution : Brandeis University *F email : KANEKO@BINAH.CC.BRANDEIS.EDU *F FBgu ID : FBgu0000020 *F date_updated: *F Oct 4 1996 7:57:51:823PM *F transposon: *F P(GAL4-per.BS) *F vector: *F pP(CaSpeR-4) *F regulatory_gene: *F per/period *F insert_size: *F 4.2kb *F Additional comments about construction: *F 4.2kb untranscribed sequence immediately upstream of the transcription *F start site to +25 site (25 bp into per mRNA). *F visualization: *F via P(UAS-lacZ.B) *F expression: *F putative per-expressing neurons in the adult brain *F lateral neurons, dorsal neurons *F Hofbauer-Buchner cells *F Weak expression in some photoreceptors *F Specific neurons in the brain in which endogenous PER has not been detected, such as neurons in central complex and pars intercerebralis. *F insertion_symbol: *F P(GAL4-per.BS-2) *F chromosome: *F 2 *F insertion_property: *F lethal # *U FBrf0091572 *a Schubiger *b G. *t 1997.3.14 *T personal communication to FlyBase *u *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000003 *F date_updated: *F Sep 23 1996 6:19:43:930PM *F GawB: *F Yes *F insertion_symbol: *F 2xPE-1 GAL4 *F chromosome: *F 2 *F within_gene: *F Not located *F polytene: *F Not located *F expression: *F For PE expression pattern see Jiang and Levine, Cell 72:741-752, 1993. *F insert_comments: *F Bam HI and Xba I were used to remove Beta Gal and replace it with GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000004 *F date_updated: *F Sep 23 1996 6:23:19:536PM *F GawB: *F Yes *F insertion_symbol: *F 2xPE-2 GAL4 *F chromosome: *F 3 *F within_gene: *F Not located *F polytene: *F Not located *F expression: *F For PE expression pattern see Jiang and Levine Cell 72:741-752, 1993. *F insert_comments: *F Using Bam HI and Xba I to remove Beta Gal and replace GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000005 *F date_updated: *F Sep 23 1996 6:26:19:196PM *F GawB: *F Yes *F insertion_symbol: *F 2xPE-3 GAL4 *F chromosome: *F 3 *F within_gene: *F Not located *F polytene: *F Not located *F phenotype: *F Small white line in abdominal tergite in AP axis *F expression: *F For PE expression pattern see Jiang and Levine Cell 72:741-752, 1993. *F insert_comments: *F Using Bam HI and Xba I to remove Beta Gal and replace with GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000006 *F date_updated: *F Sep 23 1996 6:33:22:003PM *F GawB: *F No *F transposon: *F NEE::GAL4-1 *F chromosome: *F 2 *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000007 *F date_updated: *F Sep 23 1996 6:34:44:766PM *F GawB: *F No *F transposon: *F NEE::GAL4-2 *F chromosome: *F 3 *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000008 *F date_updated: *F Sep 23 1996 6:38:36:716PM *F GawB: *F No *F transposon: *F NEE::GAL4-3 *F chromosome: *F 3 *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000009 *F date_updated: *F Sep 23 1996 6:39:36:693PM *F GawB: *F No *F transposon: *F NEE::GAL4-4 *F chromosome: *F 1 *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000010 *F date_updated: *F Sep 23 1996 6:42:20:150PM *F GawB: *F No *F transposon: *F NEE::GAL4-5 *F chromosome: *F 2 and 3, multiple in *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000011 *F date_updated: *F Sep 23 1996 6:43:21:986PM *F GawB: *F No *F transposon: *F NEE::GAL4-6 *F chromosome: *F 3 *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 *F lastname : Schubiger *F givenname : Gerold *F institution : University of Washington *F email : gerold@u.washington.edu *F FBgu ID : FBgu0000012 *F date_updated: *F Sep 23 1996 6:44:38:880PM *F GawB: *F No *F transposon: *F NEE::GAL4-7 *F chromosome: *F 3 *F insert_comments: *F 550 bp of the rhomboid promoter (-2.2 to -1.5) was fused to GAL4 # *U FBrf0091573 *a Van Doren *b M. *t 1997.3.14 *T personal communication to FlyBase *u *F lastname : Van Doren *F givenname : Mark *F institution : Lehmann Lab NYU Medical Center Skirball Institute *F email : vandoren@saturn.med.nyu.edu *F FBgu ID : FBgu0000014 *F date_updated: *F Oct 1 1996 4:35:23:260PM *F transposon: *F PGAL4-nos.UTR *F vector: *F pPCaSpeR-4 *F regulatory_gene: *F nanos promoter and 5' and 3' UTR's *F insert_size: *F 5.1 kb *F known_reg: *F nanos promoter and 5' and 3' UTR's *F vector_comments: *F An identical construct is available using GAL4-VP16 in place of GAL4. GAL4-VP16 is a much more potent transcriptional activator that GAL4 (too good for some applications). *F visualization: *F via PUAS-lacZ.B and PUAS-GFPS65T *F expression: *F The nanos promoter should induce maternal nurse cell expression, and *F also zygotic germ cell expression after the end of embryogenesis. The nanos *F 5' and 3' untranslated regions should cause the GAL4 mRNA to be localized *F to the posterior pole and to be translated only at the posterior pole. The *F observed pattern of expression is consistent with this in that GAL4 *F activity is found preferentially in the pole cells. There is also a *F transient activity found in the posterior endoderm primordium. Expression *F of lacZ and GFP has been observed in the primordial germ cells (pole cells) *F from stg 8 (germ band extending) throughout embryogenesis and into larval *F stages. GAL4 activity also appears to be present in the germ cells of the *F male testes, but does not appear present in the nurse cells. *F There appears to be consistent 'background' staining later in *F development beginning after stg 12, and this is most likely due to aberrant *F zygotic expression from the truncated nanos promoter as it is seen in *F several lines. *F reporter_comments: *F An identical transposon was generated using GAL4-VP16 in place of GAL4. *F GAL4-VP16 is a much more potent activator than GAL4 (too good for some *F applications). *F insertion_symbol: *F PGAL4-nos.UTRB5-1 *F chromosome: *F 3 *F insertion_property: *F lethal *F insertion_symbol: *F PGal4-nos.UTRB13-1 *F chromosome: *F 3 *F insertion_property: *F viable # *U FBrf0091574 *a Yang *b M. *t 1997.3.14 *T personal communication to FlyBase *u *F lastname : Yang *F givenname : Mingyao *F institution : Division of Molecular Genetics, Univ of Glasgow, UK *F email : gbga61@udcf.gla.ac.uk *F fbrf : FBrf0082807 *F FBgu ID : FBgu0000015 *F date_updated: *F Oct 4 1996 9:49:29:616AM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}201Y *F chromosome: *F 2 *F polytene: *F 56D *F visualization: *F via P{UAS-lacZ} *F expression: *F specific staining in the Mushroom bodies of the brain. *F lastname : Yang *F givenname : Mingyao *F institution : Division of Molecular Genetics, Univ of Glasgow, UK *F email : gbga61@udcf.gla.ac.uk *F fbrf : FBrf0082807 *F FBgu ID : FBgu0000016 *F date_updated: *F Oct 4 1996 9:57:24:336AM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}30Y *F chromosome: *F 3 *F polytene: *F 70E *F expression: *F staining in the mushroom bodies of the brain *F lastname : Yang *F givenname : Mingyao *F institution : Division of Molecular Genetics, Univ of Glasgow, UK *F email : gbga61@udcf.gla.ac.uk *F fbrf : FBrf0082416 *F FBgu ID : FBgu0000017 *F date_updated: *F Oct 4 1996 10:09:24:570AM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}c232 *F chromosome: *F 3 *F polytene: *F 100B *F visualization: *F via P{USA-lacz} *F expression: *F specific staining in the ellipsoid body of the adult brain. *F lastname : Yang *F givenname : Mingyao *F institution : Division of Molecular Genetics, Univ of Glasgow, UK *F email : gbga61@udcf.gla.ac.uk *F FBgu ID : FBgu0000018 *F date_updated: *F Oct 4 1996 10:19:25:010AM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}c133 *F chromosome: *F 1 *F polytene: *F 11D *F visualization: *F via P{UAS-lacZ} *F expression: *F staining in the antennal lobe complex of the brain # *U FBrf0091575 *a Diez del Corral *b R. *t 1996.10.28 *T personal communication to FlyBase *u *F lastname : Diez del Corral *F givenname : Ruth *F institution : Centro de Biolog=EDa Molecular 'Severo Ochoa' UAM-CSIC *F email : rdiez@mvax.cbm.uam.es *F author1 : Jos=E9-Luis G=F3mez-Skarmeta *F author2 : et al *F year : 1996 *F journal : Cell *F volume : 85 *F start_page : 95 *F end_page : 105 *F FBgu ID : FBgu0000036=20 *F date_updated: *F Oct 28 1996 2:32:11:363PM *F transposon: *F P{UAS-ara} *F vector: *F pP{UAST} *F cDNA: *F ara/araucan *F dbid: *F x95179 *F insert_size: *F 2800 *F insertion_symbol: *F P{UAS-ara}29 *F chromosome: *F 3 *F insertion_property: *F viable *F insertion_symbol: *F P{UAS-ara}36 *F chromosome: *F 2 *F insertion_property: *F viable # *U FBrf0091576 *a Adler *b P. *t 1997.3.14 *T personal communication to FlyBase *u *F lastname : Adler *F givenname : Paul *F institution : University of Virginia *F email : pna@virginia.edu *F FBgu ID : FBgu0000037 *F date_updated: *F Nov 1 1996 4:17:56:670PM *F transposon: *F P{UAS-fz} *F vector: *F pP{UAST} *F cDNA: *F fz/frizzled *F insertion_symbol: *F P{UAS-fz.1} *F chromosome: *F 1 *F insertion_property: *F untested *F insertion_symbol: *F P{UAS-fz.2} *F chromosome: *F 2 *F insertion_property: *F viable *F insertion_symbol: *F P{UAS-fz.3} *F chromosome: *F 3 *F insertion_property: *F viable # *U FBrf0091591 *a Suter *b B. *t 1997.2.28 *T personal communication to FlyBase *u *F >From Beat_Suter@maclan.mcgill.ca Fri Feb 28 21:35:48 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 28 Feb 1997 21:35:48 +0000 *F Date: 28 Feb 1997 16:39:18 -0500 *F From: 'Beat Suter' *F Subject: Re: Help FlyBase please *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP/QM 3.0.0 *F Content-Length: 265 *F *F Reply to: RE>Help FlyBase please *F *F Dear Michael: *F *F > Do you have a name/symbol for yr new kinase in 38B ? *F > What should FlyBase call it ? *F *F --> we did find one today: The full name is 'loki' (LOKI), the short form *F 'lok'. *F *F Thanks for pointing it out! *F *F Beat # *U FBrf0092781 *a Beaton *b A. *t 1997.3.4 *T personal communication to FlyBase *u *F ========New deficiencies: *F Df(2L)Dweewo5 *F source Shelagh Campbell. *F 27C2-3;27C4-5 *F Cyto. based upon complementation pers. comm. S. Campbell *F Df(2L)S2590 *F 23D;23F *F Cyto. done by T. Laverty *F Df(2R)RD36 *F ; *F source P. Taghert *F Df(2R)RM2-1 *F source Felix Karim *F 54F2;56A1 *F Cyto. done by T. Laverty *F Df(2R)XE3030 *F source Felix Karim *F 57C2;58C *F Cyto. done by T. Laverty *F =========New genetics for existing Dfs *F Df(2R)AA21 *F 56F9-56F17;57D12 *F bk1 must be at least at 57B4. M. Leptin *F Df(2L)E110 *F complements l(2)k04917 *F Df(2L)GpdhA *F noncomps l(2)02439 *F Df(2L)J-H *F complements l(2)02107 *F Df(2L)N22-5 *F noncomps l(2)k02506 *F Df(2L)ast1 *F 21C7-21C8;23A1-23A2 *F complements l(2)03953 *F Df(2L)dp-79b *F complements l(2)s5379 *F Df(2R)B5 *F 46A1-46A4;46C3-46C12 *F complements l(2)k03111 *F complements l(2)k08601 *F Df(2R)P34 *F 55D2-55E1;55E3-55E4 *F complements l(2)k06602 *F noncomps l(2)02029 *F Df(2R)PC29 *F 55C1-55C2;56B1-56B2 *F complements l(2)k08810 *F Df(2R)pk78k *F complements l(2)04524 *F In(2R)pk78s *F 42C7;43F5-43F8 *F complements l(2)04524 # *U FBrf0092782 *a Beumer *b K. *t 1997.3.10 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Feb 13 10:56:18 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Feb 1997 10:56:18 +0000 *F To: golic@bioscience.utah.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Thu, 13 Feb 1997 10:58:50 +0000 *F Content-Length: 1068 *F Dear Dr. Golic, *F I am a curator working for FlyBase and I am presently curating a paper *F (Frise et al., 1996, Proc. Natl. Acad. Sci. USA 93(21): 11925-11932) in *F which a UAS-FLP construct obtained from you is used. *F I would be really grateful if you could let me know the details of how this *F construct was made, for example is the FLP coding region the same as in *F your heat shock construct and is the vector used pUAST ? Alternatively is *F there a paper in which these details are published? *F As this construct is new to FlyBase I would like to get some data from you *F and/or K. Bunier, and not just a secondary publication. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From GolicLab@bioscience.utah.edu Fri Feb 14 18:11:08 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Feb 1997 18:11:08 +0000 *F Date: 14 Feb 1997 11:15:14 U *F From: 'GolicLab' *F Subject: Re: FWD>FlyBase query *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.1 *F Content-Length: 2372 *F Reply to: RE>FWD>FlyBase query *F Gillian, I am the person who made this construct, and I'll be glad to send *F you details. I won't have time till next week, but want you to know I was on *F it. By the way, my name is badly misspelled in this paper. It is actually K *F Beumer, not Bunier. Kelly *F \-------------------------------------- *F Date: 2/13/97 9:42 AM *F To: GolicLab *F From: Golic *F Kelly - can you help this person out? *F Kent *F \-------------------------------------- *F Date: 2/13/97 4:03 AM *F From: Genetics *F Dear Dr. Golic, *F I am a curator working for FlyBase and I am presently curating a paper *F (Frise et al., 1996, Proc. Natl. Acad. Sci. USA 93(21): 11925-11932) in *F which a UAS-FLP construct obtained from you is used. *F I would be really grateful if you could let me know the details of how this *F construct was made, for example is the FLP coding region the same as in *F your heat shock construct and is the vector used pUAST ? Alternatively is *F there a paper in which these details are published? *F As this construct is new to FlyBase I would like to get some data from you *F and/or K. Bunier, and not just a secondary publication. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F \------------------ RFC822 Header Follows ------------------ *F Received: by bioscience.utah.edu with SMTP;13 Feb 1997 04:02:02 U *F Received: from admin-sun1.gen.cam.ac.uk 131.111.46.141 *F by lilac.csi.cam.ac.uk with smtp (Exim 1.58 \#1) *F id 0vuys8-0007Oq-00; Thu, 13 Feb 1997 10:58:36 +0000 *F Received: from eddy.gen.cam.ac.uk 131.111.46.10 *F by admin-sun1.gen.cam.ac.uk with smtp (Exim 1.58 \#2) *F id 0vuyps-0005qM-00; Thu, 13 Feb 1997 10:56:16 +0000 *F Received: from gm119 by eddy.gen.cam.ac.uk with local (Exim 1.58 \#1) *F id 0vuysL-00009z-00; Thu, 13 Feb 1997 10:58:50 +0000 *F To: golic@bioscience.utah.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F Message-Id: *F From: Gillian Millburn (Genetics) *F Date: Thu, 13 Feb 1997 10:58:50 +0000 *F From GolicLab@bioscience.utah.edu Mon Mar 10 23:16:01 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 10 Mar 1997 23:16:01 +0000 *F Date: 10 Mar 1997 16:21:33 U *F From: 'GolicLab' *F Subject: Re: FlyBase query to Kelly *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.1 *F Content-Length: 697 *F Reply to: RE>>FlyBase query to Kelly Beumer *F Gillian, *F I am sorry it took me so long to get back to you. I was meeting deadlines. *F My p UASFLP is marked with ry and is constructed as follows. FLP, with the 2 *F micron poly A tail was excised from pFV17, the same source as for hsFLP and *F inserted into pUAST as an Xba-Sal fragment. It was then lifted as a Bam *F fragment and put in an intermediate vector, pHSS6, which allowed excison as *F an Xba fragment and insertion into pDM23, a Carnagie vector derivative. I *F have a map in Macplasmap, if that would be helpful, that I could forward to *F you. *F Kelly # *U FBrf0092793 *a Hafen *b E. *t 1991.5.6 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Ernst Hafen, Universitat Zurich *F To: Bloomington Drosophila Stock Center *F Subject: P{sev4} *F Dated: 6 May 1991 *F *F Background: P{sev4} is an unpublished construct present in three transposon stocks contributed to the collection by Ernst Hafen. *F *F Information communicated: *F P{sev4} (progenitor pW8) carries a wild-type sev cDNA driven by two copies of the sev enhancer. # *U FBrf0092796 *a Hiromi *b Y. *c S.J. *d Butler *t 1997.3.8 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Mar 04 13:04:03 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 4 Mar 1997 13:04:03 +0000 *F To: yhiromi@lab.nig.ac.jp *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 4 Mar 1997 13:06:53 +0000 *F Content-Length: 1178 *F Dear Dr. Hiromi, *F I am curating your paper: *F Butler et al., 1997, Development, 124: 781--792 *F for FlyBase. *F FlyBase currently has the name of the gene that the H214 insertion is *F located in as h214. This name will be changed to klingon (klg). *F I am writing to ask you what the relationship between klingon and the gene *F E(sev)3D is. In your abstract from the 35th Annual Drosophila Research *F Conference 1994 (page 231) you say that h214 (i.e. klingon) and E(sev)3D *F may be identical genes, since recessive lethal mutations of h214 do not *F complement a recessive lethal allele of E(sev)3D. I would be grateful if *F you could tell me whether klingon and E(sev)3D are the same gene or whether *F they are in fact two separate genes ? *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From gm119@gen.cam.ac.uk Tue Mar 04 14:22:04 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 4 Mar 1997 14:22:04 +0000 *F To: yhiromi@lab.nig.ac.jp *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 4 Mar 1997 14:24:49 +0000 *F Content-Length: 1252 *F Dear Dr. Hiromi, *F I have another question about your paper: *F Butler et al., 1997, Development, 124: 781--792 *F In the paper you use S2 cells that express a Neuroglian construct (S2-Nrg *F cells) and you reference the paper: *F Hortsch et al., 1995, J. Biol. Chem. 270(32): 18809--18817 *F Three Neuroglian constructs are mentioned in the above paper. They are: *F 1) A construct in which the Neuroglian 167 splice variant is expressed *F under the control of a metallothionein promoter. *F 2) A construct in which the Neuroglian 180 splice variant is expressed *F under the control of a metallothionein promoter. *F 3) A construct in which Neuroglian sequences are fused to Fasciclin 1 *F sequences and expressed under the control of a metallothionein promoter. *F I would be grateful if you could tell me which construct was in the cells *F you used in your paper, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From yhiromi@lab.nig.ac.jp Sat Mar 08 00:00:55 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 8 Mar 1997 00:00:55 +0000 *F X-Mailer: Macintosh Eudora Pro Version 2.1.3-J *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='ISO-2022-JP' *F Date: Sat, 8 Mar 1997 09:03:20 +0900 *F To: Gillian Millburn (Genetics) *F From: yhiromi@lab.nig.ac.jp (Yasushi Hiromi) *F Subject: Re: FlyBase query *F Cc: sb457@columbia.edu *F Content-Length: 4022 *F Dear Dr. Millburn, *F Thank you very much for your mails regarding our paper: *F Butler et al., 1997, Development, 124: 781--792 *F (1) The relationship between klingon and the gene E(sev)3D. *F >I would be grateful if you could tell me whether klingon and E(sev)3D are t *F >he same gene or whether *F > they are in fact two separate genes ? *F A short answer to your query is that they are two separate genes. *F As is described in the 35th Annual Drosophila Research Conference 1994 (page *F 231), we had postulated that h214 (i.e. klingon) and E(sev)3D may be identic *F al genes based on the following observations: *F (1) The E(sev)3De0Q mutation enhances the R7-loss phenotype of a hypomorph *F ic allele of sevenless (sevB4). Ref. Simon et al. (1991). *F (2) The H214 enhancer trap strain and the klingon gene are expressed in the *F R7 neuron. *F (3) Deletions that remove part or all of the klingon gene fails to compleme *F nt the lethality of the E(sev)3De0Q chromosome (the screen done by Mike Si *F mon identified only one allele of this locus). *F However, we later found out that the lethality of E(sev)3De0Q (with respe *F ct to the failure to complement klingon mutations) can be separated from the *F enhancement of the sevB4 phenotype. Thus the E(sev)3De0Q chromosome con *F tains two unrelated hits, one that is uncovered by the deletion of the kling *F on gene (which we mapped to 3-78.1), and the other hit maps to the E(sev)3D *F locus (3-97.3). *F I would like to have the previous Flybase information deleted, or corrected *F citing the above mentioned recombination data (S.J. Butler and Y. Hiromi, *F unpublished, or S. J. Butler (1996), Ph. D. Thesis, Princeton University). *F (2) S2 cells that express a Neuroglian construct (S2-Nrg cells) *F The construct used in our work is: *F 2) A construct in which the Neuroglian 180 splice variant is expressed under *F the control of a metallothionein promoter. *F This variant is also called the 'neuron specific form'. We should have spec *F ified it clearly in our paper. *F Sincerely yours, *F Yasushi Hiromi *F ____________________________________________ *F At 10:06 PM 97.3.4, Gillian Millburn (Genetics) wrote: *F > Dear Dr. Hiromi, *F > *F > I am curating your paper: *F > *F > Butler et al., 1997, Development, 124: 781--792 *F > *F > for FlyBase. *F > *F > FlyBase currently has the name of the gene that the H214 insertion is *F > located in as h214. This name will be changed to klingon (klg). *F > *F > I am writing to ask you what the relationship between klingon and the gene *F > E(sev)3D is. In your abstract from the 35th Annual Drosophila Research *F > Conference 1994 (page 231) you say that h214 (i.e. klingon) and E(sev)3D *F > may be identical genes, since recessive lethal mutations of h214 do not *F > complement a recessive lethal allele of E(sev)3D. I would be grateful if *F > you could tell me whether klingon and E(sev)3D are the same gene or whether *F > they are in fact two separate genes ? *F > *F > I look forward to hearing from you, *F > *F > Gillian Millburn *F > I have another question about your paper: *F > *F > Butler et al., 1997, Development, 124: 781--792 *F > *F > In the paper you use S2 cells that express a Neuroglian construct (S2-Nrg *F > cells) and you reference the paper: *F > *F > Hortsch et al., 1995, J. Biol. Chem. 270(32): 18809--18817 *F > *F > Three Neuroglian constructs are mentioned in the above paper. They are: *F > *F > 1) A construct in which the Neuroglian 167 splice variant is expressed *F > under the control of a metallothionein promoter. *F > *F > 2) A construct in which the Neuroglian 180 splice variant is expressed *F > under the control of a metallothionein promoter. *F > *F > 3) A construct in which Neuroglian sequences are fused to Fasciclin 1 *F > sequences and expressed under the control of a metallothionein promoter. *F > *F > I would be grateful if you could tell me which construct was in the cells *F > you used in your paper, *F ____________________________________________________________ *F Yasushi Hiromi *F National Institute of Genetics *F 1111 Yata, Mishima *F Shizuoka 411, JAPAN *F phone: +81-559-81-6767 *F FAX: +81-559-81-6768 *F e-mail: yhiromi@lab.nig.ac.jp *F From gm119@gen.cam.ac.uk Mon Mar 10 09:27:53 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 10 Mar 1997 09:27:53 +0000 *F To: yhiromi@lab.nig.ac.jp *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Mon, 10 Mar 1997 09:30:48 +0000 *F Content-Length: 413 *F Dear Dr. Hiromi, thankyou for your e-mail, it was very helpful. I will *F curate the information in your e-mail about klingon and E(sev)3D as a *F personal communication from you and S.J. Butler, which will correct the *F information we currently have in FlyBase. The changes may not be visible *F on the web pages immediately, as the database is updated periodically. *F Thankyou once again for your help, *F Gillian Millburn # *U FBrf0092802 *a Klambt *b C. *t 1997.2.20 *T personal communication to FlyBase *u *F >>From rd120@gen.cam.ac.uk Mon Jan 20 11:23:44 1997 *F >Envelope-to: rd120@gen.cam.ac.uk *F >Delivery-date: Mon, 20 Jan 1997 11:23:44 +0000 *F >To: cklaemb@novell.biolan.uni-koeln.de *F >Subject: helping FlyBase: one more balancer *F >Cc: rd120@gen.cam.ac.uk *F >From: Rachel Drysdale (Genetics) *F >Date: Mon, 20 Jan 1997 11:21:05 +0000 *F >Content-Length: 911 *F > *F >Hello Christian, *F > *F >I'm almost finished the balancers job, but have one last question on the *F >subject for you. In *F >*x FBrf0053395 == Klambt et al., 1991, Cell 64: 801--815 *F >you describe an FM7c chromosome marked with a P{ry+;ftz/lacZ}. *F > *F Hi Rachel ... *F the FM7c P{ry+;ftz/lacZ} I got from Yash Hiromi and I think it is a *F P{FZ}but I am not absolutely sure on this. *F Christian # *U FBrf0092804 *a Marti-Subirana *b A. *c R. *d Holmgren *t 1997.2.18 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Feb 18 15:19:02 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Feb 1997 15:19:02 +0000 *F To: martia@physlog-po.physlog.uiowa.edu *F Subject: FlyBase query *F Cc: holmgren@casbah.acns.nwu.edu, gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 18 Feb 1997 15:21:37 +0000 *F Content-Length: 1323 *F Dear Dr. Marti-Subirana, *F I am curating a paper for FlyBase (Buescher and Chia, 1997, Development 124 *F (3) 673-681) in which a TaulacZ cassette obtained from you and R. Holmgren *F is used. *F I would be really grateful if you could let me know the details of how the *F cassette is constructed, for example, *F 1) what promoter drives expression of the taulacZ fusion gene? *F 2) what is the nature of the taulacZ fusion gene - is the tau gene used *F bovine tau, and which amino acids/domains from tau and lacZ are present? *F 3) what is the marker gene in the FRT cassette? *F 4) where is the FRT cassette located within the taulacZ gene - is it *F between the promoter and the taulacZ coding region? *F Also, is there a paper in which these details are published? *F As this construct is new to FlyBase I would like to get some data from you *F and/or R. Holmgren, and not just a secondary publication. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From r-holmgren@nwu.edu Tue Feb 18 16:02:00 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Feb 1997 16:02:00 +0000 *F X-Sender: holmgren@casbah.acns.nwu.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 18 Feb 1997 10:04:23 -0600 *F To: Gillian Millburn (Genetics) *F From: Robert Holmgren *F Subject: Re: FlyBase query *F Content-Length: 559 *F Dear Gillian Millburn, *F The 'flip-out construct is described in Buenzow and Holmgren (1995) *F Developmental biology 170:338-349. *F Our paper using this construct is being resubmitted for publication. *F 1) promoter: actin5C *F 2) The tau-lacZ is from Callahan and Thomas (1994) PNAS 91: 5972-5976. *F 3) There is no marker in the 'flip-out cassette', though there is a *F Not 1 cloning site for that purpose. *F 4) The 'flip-out' cassette is between the promoter and the coding *F sequences for tau-lacZ. *F \-- *F Robert Holmgren *F Northwestern University *F r-holmgren@nwu.edu *F From MartiA@physlog-po.physlog.uiowa.edu Tue Feb 18 16:20:48 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Feb 1997 16:20:48 +0000 *F From: MartiA@physlog-po.physlog.uiowa.edu *F To: Gillian Millburn (Genetics) *F Date: Tue, 18 Feb 97 10:21 CST *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Transfer-Encoding: QUOTED-PRINTABLE *F Content-Length: 2497 *F Dear Dr. Millburn, *F The fragment containing the in-frame fusion between the bovine tau cDNA and= *F the *F lacZ gene was obtained from Callahan and Thomas (PNAS 91: 5972-5976, 1994) = *F and *F cloned into the unique SalI site of the Carnegie20/Actin5C/FRT cassete vect= *F or *F (Buenzow and Holmgren, 1995, Dev. Biol. 170:338-349). *F To answer your specific questions: *F 1) the actin 5C promoter *F 2) refer to Callahan and Thomas (1994) *F 3) the A5C/FC/taulacZ is marked with ry+ *F 4) the FRT sites are located within the promoter and the reporter taulacZ g= *F ene *F You will find details of both in the references mentioned above. *F We have recently submitted a paper in which the construct is detailed and t= *F hat *F would be the reference for the construct. I guess it has to be referred to= *F as *F =22unpublished result=22 until it is published. I could send you the refere= *F nce for *F the paper when it is accepted for publication. *F Please let me know if I can be of further help, *F Anna Marti-Subirana *F ___________________________________________________________________________= *F ____ *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) at internet *F Date: 2/18/97 3:21 PM *F Dear Dr. Marti-Subirana, *F I am curating a paper for FlyBase (Buescher and Chia, 1997, Development 124 *F (3) 673-681) in which a TaulacZ cassette obtained from you and R. Holmgren *F is used. *F I would be really grateful if you could let me know the details of how the *F cassette is constructed, for example, *F 1) what promoter drives expression of the taulacZ fusion gene? *F 2) what is the nature of the taulacZ fusion gene - is the tau gene used *F bovine tau, and which amino acids/domains from tau and lacZ are present? *F 3) what is the marker gene in the FRT cassette? *F 4) where is the FRT cassette located within the taulacZ gene - is it *F between the promoter and the taulacZ coding region? *F Also, is there a paper in which these details are published? *F As this construct is new to FlyBase I would like to get some data from you *F and/or R. Holmgren, and not just a secondary publication. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119=40gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0092808 *a Olsen *b D. *c B. *d Jordan *e D. *f Chen *g D. *h Cavener *t 1997.2.14 *T personal communication to FlyBase *u *F From dcavener@ctrvax.Vanderbilt.Edu Fri Feb 14 17:02:39 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 14 Feb 1997 17:02:39 +0000 *F Date: Fri, 14 Feb 1997 10:52:06 -0500 *F From: 'Douglas R. Cavener' *F Subject: dGCN2 *F To: flybase-updates@morgan.harvard.edu *F Reply-to: Box@ctrvax.Vanderbilt.Edu, 1820@ctrvax.Vanderbilt.Edu, *F Station@ctrvax.Vanderbilt.Edu, B@ctrvax.Vanderbilt.Edu, *F of@ctrvax.Vanderbilt.Edu, Molecular@ctrvax.Vanderbilt.Edu, *F Biology@ctrvax.Vanderbilt.Edu, Vanderbilt@ctrvax.Vanderbilt.Edu, *F University@ctrvax.Vanderbilt.Edu, Nashville@ctrvax.Vanderbilt.Edu, *F TN@ctrvax.Vanderbilt.Edu, 37235@ctrvax.Vanderbilt.Edu *F Organization: Vanderbilt University *F MIME-version: 1.0 *F X-Mailer: Mozilla 2.01 (Macintosh; U; PPC) *F Content-Type: text/plain; charset=us-ascii *F Content-transfer-encoding: 7bit *F X-Url: *F http://flybase.bio.indiana.edu:82/docs/flydocs/flybase/.refman-html/refmankm-11.3.html *F Content-Length: 869 *F My lab has recently cloned and sequenced the Drosophila homolog of the *F eIF-2alpha kinase GCN2. It contains two major domains: a catalytic *F domain containing the hallmark unique residues of eIF-2alpha kinases and *F HisRS domain homologous to histidyl-tRNA synthetase. These two domains *F define yeast (S. cerevisiae) GCN2. We have named the Drosophila homolog *F of GCN2 as dGCN2. dGCN2 maps to 100C1-3 based upon in situ *F hybridization to salivary chromosomes and mapping to ordered P1 phage *F clones. Mapping to several P1 phage clones places dGCN2 in STS Dm2514. *F All of these data are unpublished; however you have my permission to *F include any or all of the above information in the appropriate FlyBase *F files prior to publication. The unpublished reference is Olsen, D., *F Jordan, B., Chen, D. and D. Cavener, Department of Molecular Biology, *F Nashville, TN 37235. # *U FBrf0092810 *a Pereira *b A. *c M. *d Heck *t 1997.3.18 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Andrea Pereira, University of California, and Margarete Heck, University of Edinburgh *F To: Bloomington Drosophila Stock Center *F Subject: Identity of Klp61F alleles *F Dated: 18 March 1997 *F *F Information communicated: *F Klp61F[1] = l(3)06345[06345] *F Klp61F[2] = l(3)06836[06836] *F Klp61F[3] = l(3)07012[07012] # *U FBrf0092813 *a Roote *b J. *t 1996.8 *T personal communication to FlyBase *u *F \*a Df(2L)II30 *F \*G l(2)35Fd << bk1 << l(2)35Fb,l(2)35Fc,cact,cni,fzy << bk2 << chif,l(2)35Fe,l(2)35Fg *F \*a Df(2L)III18 *F \*G l(2)35Fb,l(2)35Fc << bk1 << cact,cni,fzy << bk2 << chif,l(2)35Fe,l(2)35Fg *F \*a Df(2L)A47 *F \*G l(2)34De,l(2)34Dg << bk1 << l(2)34Eb << Adhr << bk2 << l(2)35Bb *F \*a Df(2L)ARR1 *F \*G pu << bk1 << elA << Su(H),l(2)35Bg << bk2 << ck *F \*a Df(2L)TE35D-2 *F \*G l(2)35Cd << bk1 hits esg << ms(2)35Eb << bk2 << BicC *F \*a Df(2L)b87e25 *F \*G bk1 << l(2)34Da << vas << bk2 << stc *F \*a Df(2L)b81f2 *F \*G l(2)34Db << bk1 << Sos << Adhr << bk2 *F \*a Df(2L)b80k *F \*G bk1 << l(2)34Da << l(2)35Bd << bk2 << Su(H),l(2)35Bg *F \*a Df(2L)b88h49 *F \*G bk1 << l(2)34Da << elB << bk2 << pu *F \*a Df(2L)b88g96 *F \*G l(2)34Da << bk1 << l(2)34Db << l(2)34Fa << bk2 << wb *F \*a Df(2L)b88f43 *F \*G bk1 << l(2)34Da << l(2)34Fa << bk2 << wb *F \*a Df(2L)b88c58 *F \*G bk1 << l(2)34Da << stc << bk2 << l(2)35Cc,rd *F \*a Df(2L)b88c25 *F \*G bk1 << l(2)34Da << Adhr << bk2 << l(2)35Bb *F \*a Df(2L)osp29 *F \*G Adhr << bk1 << osp,l(2)35Bb << BicC << bk2 << l(2)35Fa *F Tp(2;Y)b88b15 is a 3 break event (whether visible cytologically or not): *F one to the left of 34Da, one between rk and 34Fa, one between BicC and 35Fa *F and one on the Y (4 break event). Chromosome 2 has been deleted for *F 34Da-BicC, but 34Fa-BicC has been transposed onto the Y, therefore in the *F Tpb88b15 stock females are deleted for 34Da-BicC, males are deleted for *F 34Da-rk. *F \*a Df(2L)el94 *F \*o spontaneous *F \*G l(2)35Aa << bk1 << el << pu << bk2 << noc *F \*w Roote *F \*a Df(2L)H60-3 *F \*w Tower *F \*o male recombination *F \*G l(2)35Fb, l(2)35Fc << bk1 << l(2)35Ff << cact << bk2 << chif, l(2)35Fe *F Df(2L)Adh-nBR100 \*w Lee \*o tritium *F \*G l(2)34Fa << bk1 << wb << Adhr << bk2 << l(2)35Bb *F Df(2L)Adh-nBR102 \*w Lee \*o tritium *F \*G elA << bk1 << noc << esg << bk2 << l(2)35Da *F Df(2L)Adh-nBR103 \*w Lee \*o tritium *F \*G l(2)34Fa << bk1 << wb << l(2)35Dc << bk2 << l(2)35Dd *F Df(2L)Adh-nBR105 \*w Lee \*o tritium *F \*G noc << bk1 << osp << stc << bk2 << l(2)35Cc, rd *F Df(2L)Adh-nBR106 \*w Lee \*o tritium *F \*G elA << bk1 << noc << ck << bk2 << l(2)35Cf *F (comment: breaks within noc) *F Df(2L)Adh-nBR107 \*w Lee \*o tritium *F \*G noc << bk1 << osp << ck << bk2 << l(2)35Cf *F Df(2L)Adh-nBR118 \*w Lee \*o tritium *F \*G elA << bk1 << noc << esg << bk2 << l(2)35Da *F Df(2L)Adh-nBR119 \*w Lee \*o tritium *F \*G elA << bk1 << noc << Adhr << bk2 l(2)35Bb *F (comment: breaks within noc) *F Df(2L)Adh-nBR120 \*w Lee \*o tritium *F \*G l(2)34Fa << bk1 << wb << l(2)35Ea << bk2 << ms(2)35Eb *F Df(2L)Adh-nBR121 \*w Lee \*o tritium *F \*G noc << bk1 << osp << Adhr << bk2 << l(2)35Bb *F Df(2L)Adh-nBR122 \*w Lee \*o tritium *F \*G noc << bk1 << osp << stc << bk2 << l(2)35Cc, rd *F Df(2L)Adh-nBR125 \*w Lee \*o tritium *F \*G l(2)34Fa << bk1 << wb << esg << bk2 << l(2)35Da *F Df(2L)Adh-nBR126 \*w Lee \*o tritium *F \*G elA << bk1 << noc << BicC << bk2 << l(2)35Fa *F (comment: breaks within noc) *F Df(2L)Adh-nBR127 \*w Lee \*o tritium *F \*G noc << bk1 << osp << Adhr << bk2 << l(2)35Bb *F Df(2L)Adh-nBR128 \*w Lee \*o tritium *F \*G noc << bk1 << osp << stc << l(2)35Cc, rd *F Df(2L)Adh-nBR129 \*w Lee \*o tritium *F \*G elA << bk1 << noc << l(2)35Cd << bk2 << esg *F Df(2L)Adh-nBR130 \*w Lee \*o tritium *F \*G l(2)34Da << bk1 << l(2)34Db << sna << bk2 << lace *F Df(2L)Adh-nBR131 \*w Lee \*o tritium *F \*G rk << bk1 << l(2)34Fa << stc << bk2 << l(2)35Cc, rd *F Df(2L)chif64 \*G fzy << bk1 << cact << dac << bk2 (breaks within cact) *F T(Y;2)el4DA80P \*G l(2)35Aa << bk1 << elB << pu << bk2 << elA *F (comment: breaks within elB) *F T(Y;2)el5DA80P \*G l(2)35Aa << bk1 << elB << pu << bk2 << elA *F (comment: breaks within elB) *F T(Y;2)el4DR15P \*G l(2)35Aa << bk1 << elB << l(2)35Bg, Su(H) << bk2 << *F ck (comment: breaks within elB) *F In(2LR)el6LA379R \*G l(2)35Aa << bk1 << elB << Adhr << bk2 << osp *F (comment: breaks within elB) *F T(2;3)dpps19Del24P \*G l(2)35Aa << bk1 << elB << elA << bk2 << noc *F (comment: breaks within elB) *F Df(2L)fn12 \*G bk1 << rk << ck << bk2 *F Df(2L)fn15 \*G bk1 << el << ck << bk2 *F Df(2L)GT5 \*G bk1 << b << noc << bk2 *F T(Y;2)GT2DA80P \*G l(2)35Aa << bk1 << elB << pu << bk2 << elA *F Df(2L)noc11 \*G j << bk1 << rk << l(2)35Cd << bk2 << esg *F (comment: breaks within rk) *F Df(2L)nNXF1 \*G elA << bk1 << noc << l(2)35Cb << bk2 << l(2)35Cc,rd *F (comment: breaks within noc) *F \*a Df(2L)osp38 *F \*G l(2)34Fa << bk1 << wb << esg << bk2 << l(2)35Da *F \*a Df(2L)osp144 *F \*G elA << bk1 hits noc << osp << bk2 << Adh *F \*a Df(2L)RA5 *F \*G l(2)35Dh,l(2)35Ea << bk1 << ms(2)35Eb << chif, l(2)35Fe, l(2)35Fg << bk2 << dac *F \*w Y. Hiromi *F \*O cactE10 *F \*o radiation *F \*a Df(2L)rd9 *F \*G stc << bk1 << l(2)35Cc,rd << bk2 << l(2)35Cd *F \*O Sco *F \*a Df(2L)RI1 *F \*w Y. Hiromi *F \*O cactE10 *F \*o radiation *F \*a Df(2L)RM5 *F \*w Y. Hiromi *F \*O cactE10 *F \*o radiation *F \*a Df(2L)RN2 *F \*w Y. Hiromi *F \*a Df(2L)Scorv7 *F \*G Sos << bk1 << l(2)34Dc << ms(2)35Eb << bk2 << BicC *F \*a Df(2L)TE35BC-4 *F \*G Adhr << bk1 << l(2)35Bb << esg << bk2 << l(2)35Da *F \*a Df(2L)TE35BC-24 *F \*c 35B4-35B6;35E1-35E2 *F \*a T(2;4)TE35B-50 *F \*G bk1 hits noc *F \*a T(2;4)DTD22 *F \*G twe << bk2 << l(2)35Fh *F \*a Df(2L)TE35B-54L *F \*G elA << bk1 hits noc << lace << bk2 << CycE *F \*a Dp(2;3)17 *F \*q duplicates esc and l(2)34Da - l(2)34Dd, l(2)34Df *F \*a Df(2L)TE35B-14 *F \*G bk1 << elB << l(2)35Cc,rd << bk2 *F \*a In(2L)TE35B-13 *F \*G l(2)35Aa << bk1 << elB << l(2)35Ea << bk2 << ms(2)35Eb *F \*a Df(2L)TE35B-12 *F \*G elB << bk1 << pu << noc << bk2 << osp *F \*a In(2LR)TE35B-14LTE35B-4R *F \*q deleted for nec-pk *F \*a In(2LR)TE35B-14LScorv1R *F \*q deleted for noc-l(2)35Da and nec-cn. *F \*a Df(2L)TE35D-21 *F \*G l(2)35Cd << bk1 << esg << chif, l(2)35Fe, l(2)35Fg << bk2 << dac *F \*a Df(2L)TE35D-13 *F \*G l(2)35Cd << bk1 hits esg << l(2)35Fd << bk2 << l(2)35Fb *F \*a Df(2L)TE35D-19 *F \*G lace << bk1 << CycE << BicC << bk2 << l(2)35Fa *F Df(2L)b84h50 is 34Da+, 34Db-, stc-, 35Cc+. *F Df(2L): *F A217, A245, A260, A266, A445, A63 *F TE35B-11, TE35B-15, TE35B-48, TE35B-9 *F W *F b84a1 *F fn2, fn36, fn7 *F noc10, noc13 *F are all Adh-, osp-, 35Bb+ *F Df(2L)fn52 deletes Adh and osp but does not delete noc or 35Bb *F Df(2L)AntpCtxrv1 deletes l(2)35Bd and Su(H) but not l(2)35Be or l(2)35Bf *F osp << Df(2L)TE35BC-34.bk1 << l(2)35Bb *F Df(2L)b88c75.bk1 << l(2)34Da *F l(2)35Df,l(2)35Di,l(2)35Dg << Df(2L)b88c75.bk2 << l(2)35De *F l(2)35De << Df(2L)TE35BC-34.bk2 << l(2)35Dh,l(2)35Ea *F l(2)35De << In(2LR)Scorv1-DV7.bk2 << l(2)35Dh,l(2)35Ea *F Df(2L)TE35B-5 *F \*G bk1 << l(2)34Db << stc << bk2 *F Df(2L)do1 *F \*G bk1 << l(2)34Fc << sna << bk2 *F Df(2R)cnS6 *F \*G l(2)43Cb, l(2)43Cc << bk1 << l(2)43Da << fs(2)43Ei,cn << bk2 *F \*B 43C3-43C7;43F2-43F8 *F Df(2R)spleD3 *F \*G bk1 << nec << l(2)43Ea << bk2 << blow, scra *F \*B 42E3-7;43C3-7 *F Df(2R)cnS8 *F \*G l(2)43Ea << bk1 << blow, scra << fs(2)43Ei,cn << bk2 *F Df(2L)b84a8 *F \*G bk1 << l(2)34Db << noc << bk2 # *U FBrf0092820 *a Zhang *b N. *c K. *d Howard *t 1997.2.24 *T personal communication to FlyBase *u *F From yumi@panix.com Mon Feb 24 12:13:12 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 24 Feb 1997 12:13:12 +0000 *F Date: Mon, 24 Feb 1997 07:14:50 -0500 (EST) *F From: Ken Howard *F To: Rachel Drysdale *F cc: eleanor@gen.cam.ac.uk *F Subject: Re: wunen etymology *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1411 *F Here it is: *F Following is the Wunen story: *F Wunen was a marshal in the Heaven (Chinese One). Because of stealing *F God's food and harassing fairies, He was punished to the Earth as a pig. *F He lived in a tiny village and forced a village girl to married him (he was *F still very powerful compare with human). The story started from one *F daywhen a monk named Tang went to the West (India) to get the Buddhism *F bark to China, He had to pass the village where the pig occupied. *F Villagers cameout to tell the monk how bad and strong the pig was and *F hoped the monkcould do some thing about it. It happened the monk had *F student ( he is a monkey monk) named Wukong ( notice the 'Wu') who was a *F very clever, trickyand powerful monkey (all the Chinese kids love him). *F He fought with the pig using all his tricks and power and finally the pig *F surrendered. The pig agreed to go with the monk Tang and become a monk. *F monk Tong thus gave him a Buddhist name Wunen. This is his first name, *F his family name is Zhu (complicated, isn't is ?). One way to the West, *F Wunen supposed to behave himself otherwise he would be punished by the *F monkey. However, once he had a chance, he always disappeared to somewhere *F to steal food and chasing girls. Most of the time, he was falling into *F traps setting by those beautiful girl-look-like devils (I don't what is *F the English word for these creatures) and had to be rescued by the monkey. *F N. Zhang # *U FBrf0092912 *a Fehon *b R. *t 1997.3.25 *T personal communication to FlyBase *u *F >From rfehon@acpub.duke.edu Tue Mar 25 13:27:31 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 25 Mar 1997 13:27:31 +0000 *F X-Sender: rfehon@mail-rf.acpub.duke.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 25 Mar 1997 08:25:00 -0500 *F To: Michael Ashburner (Genetics) *F From: Rick Fehon *F Subject: Re: Help Flybase *F Content-Length: 1410 *F *F Michael. *F *F The sequences under accession \#'s *F U85024-34 are not Cdc42. They are for unidentified cDNA's that were *F selected by hybridization to a cosmid that served as a genetic duplication *F for a screen we performed (Fehon et al. 1997. Genetics, in press). In other *F words, they are partial sequences (EST's) that correspond to genes for *F which we are likely to have isolated mutants already. I put them in the *F database so that if anyone ever clones these cDNA's again they will know *F that we have already isolated mutations in the corresponding genes. *F *F Hope this makes sense. Let me know if it doesn't. *F *F Rick *F =========================== *F *F >From rfehon@acpub.duke.edu Tue Mar 25 14:06:50 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 25 Mar 1997 14:06:50 +0000 *F X-Sender: rfehon@mail-rf.acpub.duke.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 25 Mar 1997 09:06:00 -0500 *F To: Michael Ashburner (Genetics) *F From: Rick Fehon *F Subject: Re: Help Flybase *F Content-Length: 841 *F *F Michael. *F *F Here are the groupings for these clones (based on cross-hybridization). The *F one problem I have with the naming scheme for the clones that you mentioned *F is that we have already adopted similar names for the unknown genetic *F complementation groups from the screen (ie. l(1)18DEa, l(1)18DEb, *F l(1)18DEc) per FlyBase conventions. I am worried that if the clones are *F named similarly, then people will assume that clone-a corresponds to *F complementation group-a, etc. We do not know that this is the case! Is *F there a way to avoid this potential confusion? *F *F Group 1 *F >U85024 clone 14.511R *F >U85025 clone 2.4R *F >U85026 clone 2.4U *F >U85027 clone 5.42R *F *F Group 2 *F >U85028 clone 21.31R *F >U85029 clone 21.31U *F *F Group 3 *F >U85030 clone 6.4R *F >U85031 clone 6.4U *F *F Group 4 *F >U85032 clone 8.1R *F *F Group 5 *F >U85033 clone 9.421R *F >U85034 clone 9.421U *F *F Cheers, *F *F Rick. # *U FBrf0093303 *a Merriam *b J. *t 1997.5.9 *T personal communication to FlyBase *u *F Personal communication from: John Merriam *F To: Bloomington Drosophila Stock Center *F Dated: 09 May 1997 *F Background: Molecular and genetic information on a P construct carried *F by a stock donated to the Bloomington collection (described on a poster *F at the 1997 Drosophila Conference). *F Information communicated: *F Insert \#4, on the X chromosome, map position about 37. The insert is marked *F with y+. This insert has the Gal4-UAS sequence and promoter oriented *F to transcribe leftward out the 5' P end. *F Further description of the insert: *F Construct P 6.11 features a promoter and UAS-Gal4 oriented to transcribe *F leftward from the 5' P end. Inserts in flies are marked with genomic y+ *F expression. *F The restriction site map is *F 5'P BamHI/BglII EcoRI hsp-UAS BamHI SalI (5' y+ genomic) HindIII BglII *F PstI HindIII BamHI EcoRI PstI BglII BamHI (3' y+ genomic) SalI PstI *F HindIII 3'P *F The distance of hsp from 5'P is approximately 140 bp. The hsp-UAS insert *F from Brand and Perrimon's pUAST is about 400 bp. The genomic y+ insert, *F from Geyer and Corces, is 8 kb in length (between SalI sites) with *F transcription from left to right. 3'P is 223 bp from the right SalI *F site. # *U FBrf0093304 *a Clark *b D. *t 1997.5.23 *T personal communication to FlyBase *u *F >From clarkd@unb.ca Fri May 23 13:56:02 1997 *F X-Sender: clarkd@pop.unb.ca *F X-Mailer: Windows Eudora Light Version 1.5.4 (32) *F Mime-Version: 1.0 *F Date: Fri, 23 May 1997 14:54:56 -0300 *F To: flybase-help@morgan.harvard.edu *F From: Denise Clark *F Subject: transposon vector sequence *F *F Dear Flybase, *F A computer "restriction digest" of vector pP{DM23} shows it has 2 XbaI *F sites, although the annotations show that XbaI is a unique site in the *F polylinker (also stated in Mismer and Rubin 1987). In my experience, there *F is only one XbaI site in the polylinker (I have used it for cloning). The *F second "computer" XbaI site lies in the rosy sequence, and this is also in *F the Genbank rosy gene sequence. *F *F --Denise Clark *F *F Department of Biology *F University of New Brunswick *F Fredericton, NB *F Canada, E3B 6E1 *F *F # *U FBrf0093306 *a Perrimon *b N. *t 1997.6.4 *T personal communication to FlyBase *u *F From crosby@morgan Wed Jun 4 14:55:26 1997 *F Return-Path: *F Received: from muller.harvard.edu by morgan.harvard.edu.harvard.edu (4.1/SMI-4.1) *F id AA23252; Wed, 4 Jun 97 14:55:24 EDT *F Received: by muller.harvard.edu (SMI-8.6/SMI-SVR4) *F id OAA17216; Wed, 4 Jun 1997 14:55:08 -0400 *F Date: Wed, 4 Jun 1997 14:55:08 -0400 *F From: crosby@morgan (Madeline Crosby) *F Message-Id: <199706041855.OAA17216@muller.harvard.edu> *F To: joec@morgan *F Subject: FBrf0093306, pc from N. Perrimon *F Cc: crosby@morgan *F X-Sun-Charset: US-ASCII *F Status: R *F *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000021 *F date_updated: *F Oct 21 1996 1:42:36:090PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}h[lJ3] *F within_gene: *F hairy (h) *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -This transposon is expressed in the endogenous hairy expression pattern. *F -One should remember when using this line that there is at least a 15 minute delay between when endogenous hairy gene transcription begins and when you will get 1J3-driven Gal-4 expression via the UAS. *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000022 *F date_updated: *F Oct 21 1996 1:49:34:303PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}24B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Expressed in mesodermal territory from the time the ventral furrow is invaginating. *F -Expressed in all mesodermal derivatives during embryonic development. *F -Expression after embryonic stages in undocumented. *F insert_comments: *F -This insertion of P{GawB} is one of the earliest expressing lines identified thus far. *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000023 *F date_updated: *F Oct 21 1996 1:52:51:706PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}24B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Expressed in mesodermal territory from the time the ventral furrow is invaginating. *F -Expressed in all mesodermal derivatives during embryonic development. *F -Expressed in eye-antennal, haltere, leg and wing imaginal discs (no details regarding expression patterns available). *F insert_comments: *F -This insertion of P{GawB} is one of the earliest expressing lines identified thus far. *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000024 *F date_updated: *F Oct 21 1996 2:00:58:296PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}69B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Expressed during embryogenesis (for more info. see Brand and Perrimon [1993]). *F -Expressed widely in eye-antennal, haltere, leg and wing imaginal discs (for more info. see and Wilder and Perrimon [1995]; Development 121:477-488). *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000025 *F date_updated: *F Oct 21 1996 2:06:17:230PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}32B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct) *F expression: *F -Expressed during embryogenesis (see Brand and Perrimon[1993] for any further details). *F -Limited expression in the eye-antennal, and leg imaginal discs. *F -Expressed throughout wing blade region of wing imaginal discs. *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000026 *F date_updated: *F Oct 21 1996 2:12:41:380PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}71B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic expression is undocumented. *F -Expressed in part of dorsal and ventral, posterior wing pouch of wing imaginal discs (excluded from area around margin). *F -Very limited expression in eye-antennal imaginal discs. *F -Expressed in two dorsal spots in the leg imaginal discs. *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000027 *F date_updated: *F Oct 21 1996 2:18:23:936PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}30A *F chromosome: *F 2 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic expression is undocumented. *F -Expressed in dorsal sector of the leg imaginal discs. *F -Expressed primarily in the wing hinge region of the wing imaginal discs. *F -Expressed at dorsal and ventral edges of the eye imaginal discs. *F -Expressed in a single, central dot of the antennal discs. *F insert_comments: *F See also Ingham and Feitz[1995]Current Biology 5:432-440. *F lastname : Perrimon *F givenname : Norbert *F institution : HHMI at Harvard Medical School, Dept. of Genetics *F email : gal4@rascal.med.harvard.edu *F author1 : Andrea Brand *F author2 : Norbert Perrimon *F year : 1993 *F journal : Development *F volume : 118 *F start_page : 401 *F end_page : 415 *F FBgu ID : FBgu0000029 *F date_updated: *F Oct 21 1996 2:33:41:400PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}55B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F phenotype: *F -May be a supressor of D-raf (J.A. Kiger, personal communication) *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Embryonic expression is undocumented, but is thought not to be expressed during embryogenesis (based on the expression of UAS constructs which are generally lethal if expressed during embryogenesis). *F -Little or no expression in eye-antennal, haltere, leg or wing imaginal discs. *F -Expressed in the follicle cells of developing egg chambers. Anterior limit is just anterior to the anterior edge of oocyte. Posterior limit is >50% of the overall length of the oocyte. *F -See Brand and Perrimon (1994); Genes and Development 8:629-639 RE:oocyte expression. *F lastname : Perrimon *F givenname : Norbert *F institution : Harvard Medical School *F email : gal4@rascal.med.harvard.edu *F FBgu ID : FBgu0000041 *F date_updated: *F Nov 4 1996 1:25:50:316PM *F GawB: *F Yes *F insertion_symbol: *F P{GAwB}64B *F chromosome: *F 3 *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Expression during embryonic stages is undocumented. *F -At third instar, there is a large spot of expression near the center of the dorsal compartment. *F -In the wing disc, there is expression in the dorsal wing hinge and in much of the thoracic part of the disc. There may also be weak expression in the vein territories. *F -In the eye/antennal disc, there is little or no expression in the eye, and several small spots of expression in the antennal region. *F insert_comments: *F This insertion was generated by A. Brand *F lastname : Perrimon *F givenname : Norbert *F institution : Harvard Medical School *F email : gal4.rascal.med.harvard.edu *F FBgu ID : FBgu0000044 *F date_updated: *F Nov 22 1996 1:27:17:023PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}19B *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Expression during embryonic development is undocumented. *F -at third instar, there is broad expression in the eye/antennal and leg imaginal discs (no further details available) *F insert_comments: *F This insertion was generated by A. Brand *F lastname : Perrimon *F givenname : Norbert *F institution : Harvard Medical School *F email : gal4@rascal.med.harvard.edu *F FBgu ID : FBgu0000062 *F date_updated: *F Nov 12 1996 1:34:07:546PM *F GawB: *F Yes *F insertion_symbol: *F P{GawB}3B *F within_gene: *F unknown *F polytene: *F unknown *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic expression of this insertion has not yet been described. *F -At the third instar, there is no expression in the imaginal discs. *F insert_comments: *F This insertion was generated by A. Brand # *U FBrf0093307 *a Edwards *b K.A. *t 1997.6.18 *T personal communication to FlyBase *u *F From kedwards@hawaii.edu Thu Jun 19 00:04:31 1997 *F Date: Wed, 18 Jun 1997 18:02:54 -1000 *F From: Kevin A Edwards *F X-Sender: kedwards@uhunix3 *F To: Madeline Crosby *F Subject: Re: CaSpeR-hs *F Mime-Version: 1.0 *F Dear Lynn, *F Here is a sequence run off a GMR vector. The specific insert, a Src29A *F cDNA, was cloned after the RI site and is deleted here, so this sequence *F should be common to all GMRs. The primer was made to Casper-hs sequence *F upstream of the Xho site, pointing forward into the promoter. The sequence *F begins with a match to casper-hs up to the Xho site, about base 20. Then *F the glass pentamer begins after the Xho- all 5 repeats are perfect. This *F goes into a Sal site and polylinker (about base 200), and then the *F promoter sequence. It ends with the PCR primer B. Hay lists in *F Dev.120:2121, terminating in the EcoRI cloning site. Presumably the rest *F of GMR is identical to casper-hs. The sequence is SS but unambiguous. Hope *F it helps! If you have any trouble putting the whole plasmid together based *F on this let me know. *F GMR-Src29A promoter region, 497 bp: *F TTTGGCAGAAAGAAAAcTCGAGCCCAGTGGAAACCCTTGAAATGCCTTTAAGTCGAG *F CCCAGTGGAAACCCTTGAAATGCCTTTAAGTCGAGCCCAGTGGAAACCCTTGAAATG *F CCTTTAAGTCGAGCCCAGTGGAAACCCTTGAAATGCCTTTAAGTCGAGCCCAGTGGA *F AACCCTTGAAATGCCTTTAAGTCGACTCTAGAGGATCCGCGGCCGCGGGTACCGGGG *F GATCCGGCGAAAAGAGCGCCGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACA *F ATTCAATTCAAACAAGCAAAGTGAACACGTCGCTAAGCGAAAGCTAAGCAAATAAAC *F AAGCGCAGCTGAACAAGCTAAACAATCTGCAGTAAAGTGCAAGTTAAAGTGAATCAA *F TTAAAAGTAACCAGCAACCAAGTAAATCAACTGCAACTACTGAAATCTGCCAAGAAG *F TAATTATTGAATACAAGAAGAGAACTCTGAATAGGGAATTC *F Kevin Edwards *F =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= *F kedwards@hawaii.edu Yamamoto Behavior Genes Project *F fax 808-956-2440 CCRT, University of Hawaii *F lab 808-956-4909 Honolulu HI 96822 # *U FBrf0093814 *a Carpenter *b A.T.C. *t 1997.5.12 *T personal communication to FlyBase *u *F From atc12@mole.bio.cam.ac.uk Mon May 12 20:15:49 1997 *F To: Aubrey de Grey *F *F Replace with: *F *F Ultrastructural studies of pachytene reveal synaptonemal complex that is *F normal in structure and length and which undergoes the same changes in *F length as is observed in wild type as the cells progress through *F pachytene; chromocentral organization and chromatin condensation are *F also normal. However, no late recombination nodules were observed in the *F nine nuclei reconstructed which were between the developmental landmarks *F which demark their presence in wild type (Carpenter, personal *F communication). *F *F Thanks! *F *F On Mon, 12 May 1997, Aubrey de Grey wrote: *F *F > so should I just delete this whole sentence? if not please supply a *F > replacement *F > XX *F > *F > > \*k Ultrastructural studies of pachytene reveal an absence *F > > \*k of chromosome condensation and little synaptonemal *F > > \*k complex (Carpenter, cited in Baker et al., 1976). # *U FBrf0093815 *a Russell *b S.R.H. *t 1997.5.17 *T personal communication to FlyBase *u *F From sr120@mole.bio.cam.ac.uk Sat May 17 14:18:15 1997 *F Subject: Acetyl coA synthetase *F *F Dear Professor Ashburner *F *F Acetyl CoA synthetase maps 4Kb proximal to Eip78C. *F *F Yours obligingly *F -- *F Steve Russell *F Dept of Genetics Phone: (44) 1223 333970 *F University of Cambridge *F Downing Street Fax: (44) 1223 333992 *F Cambridge CB2 3EH # *U FBrf0093816 *a Crews *b S. *t 1997.4.8 *T personal communication to FlyBase *u *F From crews@email.unc.edu Tue Apr 08 20:12:59 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 8 Apr 1997 20:12:59 +0100 *F Date: Tue, 08 Apr 1997 15:12:01 -0400 *F From: Stephen Crews *F Subject: Tango gene *F X-Sender: crews@pop.unc.edu *F To: Flybase updates *F MIME-version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-transfer-encoding: 7BIT *F Content-Length: 660 *F I would like to update a gene name for a novel gene that is referenced as *F abstract 35B (M. Sonnenfeld et al.) in the 38th Annual Drosophila Research *F Conference volume. In the abstract we refer to the gene as Drosophila Arnt *F (darnt). We have since named it 'tango' since it is an obligate partner *F for a number of transcription factors. The acronym is 'tgo'. *F Cheers, *F Stephen Crews *F \-------------------------------------------------------------------------------- *F Department of Biochemistry and Biophysics *F The University of North Carolina at Chapel Hill *F CB#7260 FLOB *F Chapel Hill, NC 27599-7260 *F Tel: (919) 962-4380 Fax: (919) 962-3155 *F steve_crews@unc.edu # *U FBrf0093817 *a Roote *b J. *t 1997.4.9 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Apr 09 15:02:27 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Wed, 9 Apr 1997 15:02:27 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 9 Apr 1997 15:06:16 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Content-Length: 104 *F Aubrey, *F Df(1)AC2L ABR (=Df(1)13B;13F) is sd+, i.e. sd is proximal of the ABR *F breakpoint. *F John # *U FBrf0093818 *a Sur *b R. *t 1997.4.10 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093819 *a Mitchell *b K.J. *t 1997.2.17 *T personal communication to FlyBase *u *F Personal communication from: Kevin Mitchell *F To: Bloomington Drosophila Stock Center *F Dated: Feb 17, 1997 *F Background: Generation of the recombinant chromosome is described in *F Neuron 17, 203-215 (1996). *F Information communicated: In(1)N366.2LP363R has been generated by *F recombination between In(1)N366.2 and In(1)P363. The new aberration *F is deficient for 12E9-11;17C2 and has the inversion breakpoints *F 12F2-5;17C2. Another inversion, roughly In(1)2;10, is also present. *F This chromosome is named Df(1)NP5. *F Df(1)NP5 deletes Netrin-A (NetA), Netrin-B (NetB), and rutabaga (rut). *F It does not delete mushroom body defect (mud) or yolkless (yl). *F The lethality is rescued by Dp(1;f)LJ9 and Dp(1;f)LJ4. *F Hemizygous embryos display a range of phenotypes in the CNS, most notably *F a thinning or absence of the commissures in the ventral nerve cord. This *F defect can be rescued by restoring midline expression of either Netrin gene. # *U FBrf0093820 *a Mohler *b J. *t 1997.1.31 *T personal communication to FlyBase *u *F Personal communication from: Jym Mohler *F To: Bloomington Drosophila Stock Center *F Dated: Jan 31, 1997 *F Information communicated: Df(3R)GR2 breakpoints have been revised to *F 93F11-14;94D10-13 in a reanalysis of the hh-cnc region deficiencies. # *U FBrf0093821 *a Ruden *b D.M. *t 1997.4.29 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Apr 29 09:45:17 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 29 Apr 1997 09:45:17 +0100 *F To: druden@kuhub.cc.ukans.edu *F Subject: Help FlyBase - Klp38B *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 29 Apr 1997 09:45:40 +0100 *F Content-Length: 414 *F Hi, *F You may remember that we spoke after you gave your slide session at the *F Chicago meeting. In the talk you have mentioned several alleles of Klp38B *F and later when we were chatting that they were from the BDGP. We would be *F very interested in knowing which lines you have used and therefore which *F are inserted into/cause a mutant phenotype of Klp38B. *F Many thanks for the information, *F Eleanor Whitfield *F FlyBase *F From druden@kuhub.cc.ukans.edu Tue Apr 29 15:52:56 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 29 Apr 1997 15:52:56 +0100 *F Date: Tue, 29 Apr 1997 09:54:04 -0500 (UTC -5:00) *F Date-warning: Date header was inserted by KUHUB.CC.UKANS.EDU *F From: Douglas Ruden *F Subject: Re: Help FlyBase - Klp38B *F To: eleanor@gen.cam.ac.uk *F Content-Length: 807 *F Dear Eleanor: *F The P-alleles of KLP38B are: *F l(2)03552, l(2)01300, l(2)05217, l(2)02406, and l(2)05702 *F Two of them had 'k' after l(2), but I forgot which ones. *F Regards, *F Doug Ruden # *U FBrf0093822 *a Cenci *b G. *t 1997.5.2 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Apr 29 09:37:39 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 29 Apr 1997 09:37:39 +0100 *F To: santolamazza@axcasp.caspur.it *F Subject: Help FlyBase - pendolino *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 29 Apr 1997 09:38:07 +0100 *F Content-Length: 493 *F Hi, *F You may remember that I approached you at your poster at the Chicago *F meeting regarding the symbol pen for your gene pendolino. The symbol has *F already been used for penguin (first described in FBrf0064390 and assigned *F symbol pen in FBrf0085507). *F Have you decided upon another symbol? *F Please check your new symbol in FlyBase (http://flybase.bio.indiana.edu/, *F or you find it easier to you the mirror in the UK: *F http://www.embl-ebi.ac.uk/flybase/). *F Many thanks, *F Eleanor Whitfield *F FlyBase *F From cenci@axcasp.caspur.it Fri May 02 15:15:31 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 2 May 1997 15:15:31 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: eleanor@gen.cam.ac.uk *F From: cenci@axcasp.caspur.it (gianni cenci) *F Date: Fri, 2 May 1997 15:15:30 +0100 *F Content-Length: 273 *F Dear Eleanor, *F I looked through Flybase and decided to use the symbol 'peo' for my *F 'pendolino' gene. It seems like that nobody has used 'peo' so far. *F Let me know if it is OK with you *F Best regards *F Gianni Cenci *F Note that I switched to a new and persona e-mail address. # *U FBrf0093823 *a Frasch *b M. *t 1997.4.15 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Apr 10 12:02:47 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 10 Apr 1997 12:02:47 +0100 *F To: frasch@msvax.mssm.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Thu, 10 Apr 1997 12:06:12 +0100 *F Content-Length: 1487 *F Dear Dr. Frasch, *F I am curating the paper Cai and Levine, 1997, EMBO J. 16: 1732--1741 for *F FlyBase, in which a mod(mdg4) mutation obtained from you is used. *F This mutation is called E(var)93D-P142.4D15 in the EMBO paper, and is *F described in your paper: *F Azpiazu and Frasch, 1993, Genes Dev. 7: 1325--1340, where it is called *F P142delta15. *F I have a number of questions about this mutation. *F 1. In the Materials and Methods section of the 1993 paper (top of page *F 1338, second column) you state that 'delta15 and delta32 also do not *F complement the latter mutations' - the 'latter mutations' being in 'an *F uncharacterized gene upstream of tin'. I would be grateful if you could *F confirm whether this uncharacterized gene is in fact mod(mdg4). *F 2. Could you also confirm whether delta15, delta32, and the original *F P(w+)142 insertion alter the expression of both tinman and the *F uncharacterized gene (mod(mdg4)?), or if they just alter expression of one *F of the genes ? In addition, what sequences are deleted in the delta15 and *F delta32 mutants ? *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From frasch@msvax.mssm.edu Tue Apr 15 16:47:57 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 15 Apr 1997 16:47:57 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 15 Apr 1997 11:51:54 +0000 *F To: Gillian Millburn (Genetics) *F From: frasch@msvax.mssm.edu (Manfred Frasch) *F Subject: Re: FlyBase query - answers *F Content-Length: 1796 *F >Dear Dr. Frasch, *F > *F >I am curating the paper Cai and Levine, 1997, EMBO J. 16: 1732--1741 for *F >FlyBase, in which a mod(mdg4) mutation obtained from you is used. *F > *F >This mutation is called E(var)93D-P142.4D15 in the EMBO paper, and is *F >described in your paper: *F > *F >Azpiazu and Frasch, 1993, Genes Dev. 7: 1325--1340, where it is called *F >P142delta15. *F > *F >I have a number of questions about this mutation. *F > *F >1. In the Materials and Methods section of the 1993 paper (top of page *F >1338, second column) you state that 'delta15 and delta32 also do not *F >complement the latter mutations' - the 'latter mutations' being in 'an *F >uncharacterized gene upstream of tin'. I would be grateful if you could *F >confirm whether this uncharacterized gene is in fact mod(mdg4). *F It is mod(mdg4), which is also called E(var)3-93D (Dorn et al. 1993) *F > *F >2. Could you also confirm whether delta15, delta32, and the original *F >P(w+)142 insertion alter the expression of both tinman and the *F >uncharacterized gene (mod(mdg4)?), or if they just alter expression of one *F >of the genes ? In addition, what sequences are deleted in the delta15 and *F >delta32 mutants ? *F > *F P(w+)142 is inserted 27bp upstream of mod(mdg4) (based on the sequence of *F the longest (at 5') class of cDNAs obtained). delta 15 deletes from here *F into the transcribed region of mod(mdg). delta 32 deletes from here towards *F the tin transcription start site, i.e., most of the intergenic region *F between mod(mdg) and tin, but doesn't disrupt tin transcription unit - the *F breakpoint is ca. 100bp upstream of tin). Neither of the two deletions show *F an obvious reduction of tin expression, although they don't complement tin *F mutations. The expression of mod(mdg) was not tested. *F Please let me know in case you have additional questions. *F Sincerely, *F Manfred Frasch # *U FBrf0093824 *a Ashburner *b M. *t 1997.5.29 *T personal communication to FlyBase *u *F >From ma11@gen.cam.ac.uk Thu May 29 11:54:14 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 29 May 1997 11:54:14 +0100 *F To: moconnor@uci.edu, perrimon@rascal.med.harvard.edu, ma11@gen.cam.ac.uk *F Subject: Re: sugarless/kiwi/suppenkasper/UDPGDH *F Cc: flycam@gen.cam.ac.uk, flybase-harvard@morgan.harvard.edu, *F flybase-indiana@morgan.harvard.edu, flybase-ncbi@morgan.harvard.edu, *F birchler@biosci.mbp.missouri.edu, armenm@oci.utoronto.ca *F From: Michael Ashburner (Genetics) *F Date: Thu, 29 May 1997 12:10:03 +0100 *F Content-Length: 715 *F *F This from Norbert. He is correct - sugarless (sgl) it will be in FlyBase, *F since this name has publication priority. *F Michael *F ========================================================================== *F >From ma11@gen.cam.ac.uk Thu May 29 08:50:45 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 29 May 1997 08:50:45 +0100 *F To: moconnor@uci.edu, perrimon@rascal.med.harvard.edu, ma11@gen.cam.ac.uk *F Subject: sugarless/kiwi/suppenkasper/UDPGDH *F Cc: flycam@gen.cam.ac.uk, flybase-harvard@morgan.harvard.edu, *F flybase-indiana@morgan.harvard.edu, flybase-ncbi@morgan.harvard.edu, *F birchler@biosci.mbp.missouri.edu, armenm@oci.utoronto.ca *F From: Michael Ashburner (Genetics) *F Date: Thu, 29 May 1997 09:06:24 +0100 *F Content-Length: 1405 *F *F Folks *F *F I have now heard from all except Norbert. This is the situation: *F *F Four groups have sequenced the gene coding UDP-glucose dehydrogenase and given *F it four different names. *F *F Norbert (Chicago Abstract 21C): sugarless (sgl) *F Mike (Chicago Abstract 42C): suppenkasper (ska) *F Jim: Genbank: AF001310 (UDPGDH = l(2)05007) *F Armen (June issue of Development): kiwi (not, beard). *F *F FlyBase is quite agnostic. The rules say it should be *F sugarless (sgl) but we are willing to call it kiwi if Norbert *F agrees. If not, sugarless it will be, with kiwi as a synonym. *F *F As far as I can gather Jim does not care what it is called; *F Mike is happy with sgl, and we are too late for Armen to change *F in proof ! *F *F All I can say is that I hope the gene is worth the attention it *F is getting ! *F *F Michael *F ========================================================================== *F *F >From birchler@biosci.mbp.missouri.edu Tue May 27 16:37:10 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 27 May 1997 16:37:10 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 27 May 1997 10:46:46 -0600 *F To: Michael Ashburner (Genetics) *F From: Jim Birchler *F Subject: Re: FlyBase query *F Cc: moconnor@uci.edu, perrimon@rascal.med.harvard.edu, *F eleanor@gen.cam.ac.uk *F Content-Length: 2022 *F *F Dear Jim, Mike and Norbert, *F *F In the Chicago Abstracts there are abstracts from Norbert (21C) and Mike's *F (42C) groups on genes (there) called sugarless (sgl) and suppenkasper (ska) - *F both encoding UDP-glucose dehydrogenase. Eleanor determined earlier *F by consultation with Mike and Xinhua Lin in Norbert's group that *F these were the same. I now see Genbank accession AF001310 from Jim's *F group with a sequence for this enzyme and data showing it to map *F at 65D4-6 and with l(3)05007 as a mutant allele. There is a rumour of *F another group who have found a gene coding this enzyme and calling *F it beard (is this Jim's group?). *F *F Three questions: *F *F 1. Is Jim's gene = sugarless = suppenkasper ? *F *F WE JUST CALLED IT A BORING l(3)05007 or UDPGDH)--although we seriously *F considered a past suggestion of M. A. to call it Yatu (Yet Another Transcription *F Unit). We have communicated with Mike's (O'Connor) group and know that his *F insertion used to clone his version is different than l(3)05007, but the *F complete gene sequence is identical except for a few differences that are all *F compatible with being polymorphisms. However, the two insertion alleles *F complement for viability. Probing of a Southern blot with our sequence gives no *F evidence of duplicate genes. We believe that it is the same gene. *F *F 2. Can there be agreement on the name of the gene between the groups please - *F I have no view, both sugarless (sgl) and suppenkasper (ska) are available - *F sugarless (sgl, not sl, which is used for small-wing) has page number *F >priority ! beard (bea, NOT brd) would also be available, but we do not *F know of any puplication of this name. *F *F The decision is between the other two. *F *F 3. If Jim's gene is not that called beard, then there is a 4th group - *F {who?} *F *F >Michael Ashburner *F ========================================================================== *F >From moconnor@gandalf.bio.uci.edu Tue May 27 15:41:17 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 27 May 1997 15:41:17 +0100 *F X-Mailer: ccMail Link to SMTP R6.00.02 *F Date: Tue, 27 May 97 07:44:49 -0800 *F From: 'Michael O'CONNOR' *F To: *F Subject: Re: FlyBase query *F Mime-Version: 1.0 *F Content-Type: multipart/mixed; boundary='simple boundary' *F Content-Length: 1982 *F *F Dear Mike, *F The other group is Arman Manoukian in Toronto. He is calling it beard. I *F do not care what we call it sugerless is fine with me. We have a paper in press *F calling it suppenkasper and I will put a note in proof that they are all the *F same. *F *F Regards, *F *F Mike O'Connor *F ========================================================================== *F *F >From armenm@oci.utoronto.ca Wed May 28 23:57:38 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 28 May 1997 23:57:38 +0100 *F Date: Wed, 28 May 1997 18:44:54 -0400 (EDT) *F From: Armen Manoukian *F X-Sender: armenm@at *F To: Michael Ashburner *F Subject: Re: Help FlyBase *F Mime-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 525 *F *F Dear Michael *F Our paper is in press and thus limits our options on changing the name *F (it is due out in one of the June issues of Development). The name is *F 'kiwi' (Mike Levine talked us into changing the name from beard and we *F agreed). I discussed this with Mike O'Connor but he never got back to *F me. As for sugarless, the product of UDP-glucose dehydrogenase is *F UDP-glucuronate or UDP-glucuronic acid!! I don't think that the name *F sugarless is appropriate. Please let me know what I can do to help! *F Regards *F Armen *F ========================================================================== *F >From perrimon@rascal.med.harvard.edu Thu May 29 11:17:38 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 29 May 1997 11:17:38 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 29 May 1997 06:20:56 +0000 *F To: Michael Ashburner (Genetics) *F From: perrimon@rascal.med.harvard.edu (Norbert Perrimon) *F Subject: Re: sugarless/kiwi/suppenkasper/UDPGDH *F Content-Length: 969 *F *F Hi Mike, *F *F Sorry for the slow response, I was travelling. I am for following the *F rules and call it sugarless. *F *F Best wishes, *F *F Norbert # *U FBrf0093825 *a Roote *b J. *t 1997.6.2 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Mon Jun 02 14:03:26 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 2 Jun 1997 14:03:26 +0100 *F *F John, *F >Is shof (shut off) = esg ? *F Michael *F *F Yes. Gary Hime says '...I am now convinced that shof is a specific allele *F of esg. Most of the P alleles cluster just 5' of the esg message, in the *F region that Shigeo Hayashi has found a testis specific esg enhancer. Also *F esg is expressed in precisely the cell types that I see affected.......the *F ammunition P was esg[P2]' (P2 is the name that Hayashi et al. 1993 Dev *F 118:105-115 gave to an insertion from Bier et al. 1989 Genes Dev. 3 *F 1273-1287) *F *F John # *U FBrf0093826 *a Roote *b J. *t 1997.6.2 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Mon Jun 02 17:40:55 1997 *F Envelope-to: m.ashburner@gen.cam.ac.uk *F Delivery-date: Mon, 2 Jun 1997 17:40:55 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 2 Jun 1997 17:46:18 +0000 *F To: Michael Ashburner *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: data for FB *F Content-Length: 1457 *F *F 1. *F *F P element in rk. *F *F P{(w)A[R]}11P (new name rk[11P]) *F *F The stock came from Bob Levis (Seattle) who wrote '....w[1118]; *F P{(w)A[R]}11P/CyO. The direct parent of this transformant was one called *F w[1118]; P{(w)A[R]}0923 that was inserted in 41A in beta-het and gave a *F yellow-brown eye color. 11P is a red-eyed derivative in which the P[w] *F hopped to 34E. Todd Laverty did the in situs.....no hybridisation in 41A' *F *F Screen for excisions: *F w; rk[11P]/CyO; delta2-3 X w; Gla/CyO, rk -> *F total flies 24,384 *F precise excisions 522 *F imprecise excisions 54 *F *F 2. *F *F T(2;3)H147 breaks between 34Fa and wb (not a 34Fa allele) # *U FBrf0093827 *a Flores *b C. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093828 *a Shen *b C.P. *t 1997.4.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093829 *a Ruden *b D.M. *t 1997.4.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093830 *a Tabata *b T. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093831 *a Basu *b J. *t 1997.4.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093832 *a Rasooly *b R.S. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093833 *a Lukacsovich *b T. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093834 *a Clark *b R.F. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093835 *a Kuniyoshi *b H. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093836 *a Jones *b N. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093837 *a Chiu *b S.K. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093838 *a Chauvet *b S. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093839 *a Neal *b K.C. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093840 *a Clyne *b P. *t 1997.4.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093841 *a Bryant *b P.J. *t 1997.4.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093842 *a Foss *b M. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093843 *a Pearson *b A. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093844 *a Machado *b C. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093845 *a Chong *b E.E. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093846 *a Coen *b D. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093847 *a Paricio *b N. *t 1997.4.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093848 *a Hijal *b S. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093849 *a Nguyen *b T. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093850 *a Malhotra *b D. *t 1997.4.18 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093851 *a Mlodzik *b M. *t 1997.5.10 *T personal communication to FlyBase *u *F From Marek.Mlodzik@EMBL-Heidelberg.de Sat May 10 12:18:40 1997 *F *F Yes, it is l(2)07103 due to the P-element 07103. *F *F The deletion E16 is a 'jump out excision' generated by remobilising the *F P-element 07103. *F *F Hope this is helpful, *F Regards, *F Marek *F *F >Hi ! I am trying to curate your 14-3-3 zeta sequence for FlyBase. *F >I notice that it says *F > /note='integration P2-07103' *F > *F >Is this l(2)07103 at 46E4--46E8, due to P-element 07103 ? *F >Also what is deletion E16 ? *F > *F >Thanks *F > *F >Michael Ashburner, Phone: +44 1223 333969 *F >Department of Genetics, Fax: +44 1223 333992 *F >Downing Streeet, e-mail: m.ashburner@gen.cam.ac.uk *F >Cambridge, CB2 3EH, England. # *U FBrf0093852 *a Roote *b J. *t 1997.6.6 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Fri Jun 06 17:20:46 1997 *F *F Aubrey - this from John Roote, despite header. *F *F I have shown that E(var)189 is an allele of Sos, by failure *F of complementation. *F *F John Roote. # *U FBrf0093853 *a Roote *b J. *t 1997.6.3 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Jun 03 17:48:20 1997 *F *F The Formalized genetic data for deletions whose proximal breakpoints are: *F << l(2)34Dc << bk2 << *F should read: *F << l(2)34Dc << bk2 << l(2)34Dd, l(2)34Df *F *F These are: b79h1A, b81a, b83b22, b85b2, b82a3, b79d5 and b88b42 *F *F Similarly, all deletions like this: l(2)34Dg << bk1 << Ance *F should be: l(2)34De, l(2)34Dg << bk1 << Ance *F (these are TE35B-407, A47, el80i1, el82f1, AdhnBR55) *F *F and all deletions : l(2)34Dg << bk2 << Ance *F should be: l(2)34De, l(2)34Dg << bk2 << Ance *F (these are b36f, b85b1, b84a9, b83l1, b81l42, b88e45, b88g83). # *U FBrf0093854 *a Stronach *b B. *t 1997.6.18 *T personal communication to FlyBase *u *F >From Beth.Stronach@m.cc.utah.edu Tue Jun 17 23:09:23 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 17 Jun 1997 23:09:23 +0100 *F Date: Tue, 17 Jun 1997 16:13:11 -0600 (MDT) *F From: Ellen Beth Stronach *F To: Michael Ashburner *F Subject: Re: Help FlyBase please *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1579 *F *F *F Hello Dr. Ashburner: *F *F I checked my notes again, and this is correct, P725 fails to complement *F Df (4) G, 0/79. *F *F Beth Stronach # *U FBrf0093855 *a Guillemin *b K. *t 1997.6.19 *T personal communication to FlyBase *u *F >From guillemi@cmgm.Stanford.EDU Thu Jun 19 00:45:19 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 19 Jun 1997 00:45:19 +0100 *F Date: Wed, 18 Jun 1997 16:49:27 -0700 (PDT) *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Michael Ashburner (Genetics) *F From: Karen Guillemin *F Subject: Help FlyBase please *F Content-Length: 714 *F *F Dear Professor Ashburner, *F *F regarding the map position of misguided. The information you have is *F correct and you can attribute it to a personal communication form myself *F and Mark. *F *F Sincerely, *F Karen Guillemin *F *F >Dear Mark *F > *F >We are in the process of a major cleanup of all map data in FlyBase. *F > *F >We have a statement in FB that misguided maps to 25D7--26A9 & is included *F >in Df(2L)GpdhA. But this statement is unattributed (ie we have no *F >idea where it came from !). We do not like this ! I guess the *F >data is from your lab. Could you confirm it for me please (then *F >we can reference you as a personal communication). Of course, *F >if the data is published, then the reference would be great. *F > *F >Thanks *F > *F > *F >Michael # *U FBrf0093856 *a Czank *b A. *t 1997.5.14 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Apr 29 09:23:43 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 29 Apr 1997 09:23:43 +0100 *F To: czank@zool.unizh.ch *F Subject: Help FlyBase - 89B11 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 29 Apr 1997 09:24:12 +0100 *F Content-Length: 764 *F Hi, *F You may remember I approached you at your poster at the Chicago meeting *F concerning the two P-element insertions A108 and A381 into your gene *F 89B11. I wanted information about the insertions that you said you would *F send later as you did not have the details with you. *F The questions are: *F 1) What P-element vector is used for the insertions, a natural element or *F engineered element? If engineered please provide details eg is it a PZ or *F lacW insertion, did you get the lines from BGDP? *F 2) Where have the P-elements inserted relative to the gene 89B11? *F 3) Is 89B11 a temporary symbol for the gene, do you have another you want *F to use? *F And if there is anything else you think we should know then please send *F that too! *F Many thanks, *F Eleanor Whitfield *F FlyBase *F From czank@zool.unizh.ch Wed May 14 14:13:07 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 14 May 1997 14:13:07 +0100 *F Date: Wed, 14 May 97 15:16:15 +0200 *F X-Sender: czank@rzu-mailhost.unizh.ch *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Transfer-Encoding: quoted-printable *F To: Eleanor Whitfield (Genetics) *F From: czank@zool.unizh.ch (Andi Czank) *F Subject: Description of A108 and A381 *F Content-Length: 3419 *F Dear Eleanor, *F here the details about the stocks A108 and A381. Both carry a P-element *F insertion in the same gene in 89B (transheterozygotes show the same *F phenotype as homozygotes of either stock). The insertion sites are separated *F by about 5 kb. Their relative position to the gene is not known as no *F transcript has been identified so far. I am working on that. About 20 kb of *F the surrounding genomic DNA is cloned. The P1 clones DS03648 (89B11) and *F DS07922 (89B19-C1) do contain the DNA between the insertion sites. The *F insertion in A108 was mapped to 89B13-16 based on in situs on polytene *F chromosomes. The genetic mapping gave 3-58.2 which corresponds to 89B10. *F The P-element insertions show the same phenotype (see below) when placed *F over either Df(3R)sbd26 (89B9-10; 89C) or Df(3R)sbd104 (89B5; 89C) *F (Heitzler et al. (1996) Genetics 143: 1271-1286). My gene is not allelic to *F sbd. Taken together 89B11 seems to be quite accurate. *F As a new name for the gene I would like to propose sarah (abbreviation: *F sra), with the two alleles sraA108 and sraA381. To my knowledge no other *F gene is named sarah. I chose this name because Sarah, Abrahams wife, was *F infertile for many years but eventually had a child. This is also the fate *F of homozygous sraA381 females (see below). *F Genotypes (description of the P-elements see separate pages): *F A108: ry sraA108/TM3, Sb ry e *F A381: y w; lx sraA381 /TM3, Sb Ser *F A426: w; sraA381 /TM3, Sb ry e *F lx is an unidentified recessive lethal that maps to 3-47.7. To be able to *F study sraA381 homozygotes, the stock A426 was established. *F Phenotype: *F Mating induces an increase of the female's oviposition rate and reduces her *F receptivity. Sex-Peptide (SP), a male peptide pheromone transferred during *F copulation, is sufficient to elicit the two postmating reactions. *F The two sra alleles are recessive for female sterility and for the lack of *F increase of the oviposition rate after mating or SP application. The *F receptivity is slightly reduced. No effects on males have been observed. *F There are some traits unique to the individual alleles. *F sraA108: homozygous sraA108 females show a high mortality, 50% die within *F four days after eclosion. *F sraA381: homozygous sraA381 have a normal mortality, they are sterile but *F have a very few offspring if kept at 28=B0C (ten females have one or two *F offspring if left in the same tube for two weeks). *F The P-element construct in A381 and A426 *F The above figure is the only information I have. The original designation *F of the stock A381 was 288/30. This stock was made and kindly provided by *F Dr. De=E1k, Szeged, Hungary. The stocks from his collection have been *F screened for lethal P-element insertions. In my case lethality was not *F caused by the P-element which allowed the construction of the stock A426. *F The P-element construct in A108 *F The above figure is taken from Aigaki et al. (1991) Neuron 7: 557-563. A108 *F carries the third construct (YPhsSPg). Mature virgins from other stocks *F with this construct behave like mated females, i.e. laying a lot of eggs *F and refusing courting males. Mature A108 virgins do not behave this way. *F Their oviposition rate is the same as the rate of virgins without the *F construct. This is due to the P-element insertion into the sra gene. *F Note: the figures cannot be transmitted by e-mail. Please send me a fax *F number of your mailing address to provide you with the figures. *F All the best, Andy # *U FBrf0093857 *a Fogarty *b M. *t 1997.5.22 *T personal communication to FlyBase *u *F From spellman@leland.Stanford.EDU Thu May 22 19:55:44 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Thu, 22 May 1997 19:55:44 +0100 *F Date: Thu, 22 May 1997 11:52:50 +0000 *F From: Paul Spellman *F Subject: (no subject) *F To: flybase-updates@morgan.harvard.edu *F Organization: Stanford *F MIME-version: 1.0 *F X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) *F Content-Type: text/plain; charset=us-ascii *F Content-transfer-encoding: 7bit *F X-Url: *F http://flybase.bio.indiana.edu/docs/flydocs/flybase/.refman-html/refmankm-11.3.html *F Content-Length: 237 *F Dear Flybase, *F Please correct the map position of the gene one shot (osho) in your *F database. It does not map to the 36 region as stated on your web page, *F it actually maps to 38C-E by in situ hybridization. *F Thanks, Mignon Fogarty # *U FBrf0093858 *a Kopp *b A. *t 1997.5.28 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed May 28 08:35:50 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 28 May 1997 08:35:50 +0100 *F To: duncan@biodec.wustl.edu, kopp@wustlb.wustl.edu *F Subject: Help FlyBase - hhMir *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 28 May 1997 08:36:19 +0100 *F Content-Length: 669 *F Hi Ian and Artyom, *F FlyBase minimally curates from the abstracts presented at the Annual *F Drosophila Research Conference. In April, at the Chichago meeting, you *F presented a slide (21B) in which you discuss a new hh allele - Mir. *F Does this allele have any relationship to the gene Mir, Mirabile that is *F described in FlyBase and in the Red Book? *F I ask as the Red book states that Mir is not allelic to hh (other *F information section), but then you have an allele Mir of hh. If this is *F purely by chance then no problems, but if you are suggesting allelism between *F hh and Mir then could you please provide your evidence for this. *F Many thanks, *F Eleanor Whitfield *F FlyBase *F From kopp@wustlb.wustl.edu Wed May 28 20:04:18 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 28 May 1997 20:04:18 +0100 *F Date: Wed, 28 May 1997 13:58:43 -0500 (CDT) *F Mime-Version: 1.0 *F From: kopp@wustlb.wustl.edu *F To: WUGATE::'eleanor@gen.cam.ac.uk' *F Subject: RE: Help FlyBase - hhMir *F Content-Type: text/plain; charset=US-ASCII *F Content-Transfer-Encoding: 7bit *F Content-Length: 427 *F Dear Eleanor: *F hh-Mir is the same as Mir described in the Red Book, and it is indeed a gain-of- *F function allele of hh. It causes ectopic hh expression in the anterior *F compartment. Three reversions of hh-Mir have breaks at the hh locus and behave *F as hh nulls or hypomorphs. hh-Mir itself complements hh nulls, which probably *F led to the report that they are not allelic. Sorry we caused you this headache. *F Best wishes, *F Artyom # *U FBrf0093859 *a Laverty *b T. *t 1997.6.5 *T personal communication to FlyBase *u *F From: 'Kathy Matthews' *F Personal communication from: Todd Laverty, Berkeley Drosophila Genome Project *F To: Bloomington Drosophila Stock Center *F Subject: variant of CyO *F Date: 5 June 97 *F *F Information communicated: *F *F CyO-CR2, P{sevRas1.V12}* # *U FBrf0093860 *a Lung *b O. *t 1997.5.22 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu May 22 08:29:39 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Thu, 22 May 1997 08:29:39 +0100 *F To: yol1@cornell.edu *F Subject: Help FlyBase - spider neurotoxin *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 22 May 1997 08:30:00 +0100 *F Content-Length: 721 *F Hi Oliver, *F I have curated your abstract from the April Drosophila meeting in Chicago *F (45B) for FlyBase. You discuss sequence homology of Acp62F to a neurotoxin *F from the venom of Phoneutria nigriventer (Brazilian 'armed' spider). *F FlyBase maintains these homology statements through a link to the gene from *F the other species. To do this we need more information about the *F neurotoxin. I listened to your talk in which you stated Tx2 as the *F neurotoxin. A Swissprot search gives these results: *F AC P29423; *F DE NEUROTOXIN TX2-1. *F AC P29424; *F DE NEUROTOXIN TX2-5. *F AC P29425; *F DE NEUROTOXIN TX2-6. *F AC P29426; *F DE NEUROTOXIN TX2-9. *F Which neurotoxin is correct? *F Thanks for your help *F Eleanor Whitfield *F FlyBase *F From yol1@cornell.edu Fri May 23 00:30:21 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 23 May 1997 00:30:21 +0100 *F X-Sender: yol1@postoffice.mail.cornell.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 22 May 1997 19:33:37 -0400 *F To: Eleanor Whitfield (Genetics) *F From: Oliver Lung *F Subject: Re: Help FlyBase - spider neurotoxin *F Content-Length: 143 *F Hi Eleanor, *F Highest similarity is with Tx2-6. *F Oliver *F Oliver Lung *F Section of Genetics and Development *F Cornell University *F tel. (607)2544826 # *U FBrf0093861 *a O'Donnell *b J. *t 1997.6.2 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon Jun 02 11:07:35 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 2 Jun 1997 11:07:35 +0100 *F To: jodonnel@biology.as.ua.edu, elaine@mendel.berkeley.edu *F Subject: Help FlyBase - FBrf0049526 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 2 Jun 1997 11:07:56 +0100 *F Content-Length: 657 *F Hi, *F I have curated a paper of yours for FlyBase: *F FBrf0049526 == O'Donnell et al., 1989, Dev. Genet. 10: 273--286 *F in which you discuss numerous transcripts in Figure 9 (pg 281). *F Have you assigned symbols to any of these transcripts? *F The 1.7kb transcript is Pu but if you have no other symbols for the *F transcripts then they will be given the symbols anon-57C followed by the *F letter b through to q, eg anon-57Cb. *F As there are so many I was hoping you would have chosen symbols for them! *F If you have any problems with this query (as you did do the work back in *F 1989!) please let me know as soon as possible. *F Thanks for your help, *F Eleanor Whitfield *F FlyBase *F From JODONNEL@biology.as.ua.edu Mon Jun 02 15:19:09 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 2 Jun 1997 15:19:09 +0100 *F From: *F Organization: University of Alabama Dept. of Biol *F To: eleanor@gen.cam.ac.uk *F Date: Mon, 2 Jun 1997 8:29:30 CST6CDT *F Subject: Re: Help FlyBase - FBrf0049526 *F Priority: normal *F X-mailer: Pegasus Mail/Mac (v2.1.2) *F Content-Length: 1155 *F Good morning. I believe I can assist to some extent. The transcripts that *F I know about are as follows: *F 9 kb: This is a tudor transcript. *F 3, 1.75 and 1.7 all are Pu RNAs which encode different isoforms of GTP *F cyclohydrolase. The 3 kb, and the 1.75 kb are now known to share several *F 3' exons (the enzyme catalytic domain) with the 1.7 kb. The 1.75 kb is, in *F fact, two transcripts differing by a 60 bp internal exon. These are now *F known by us as Pu transcript a = GTPCH Isoform a = 1.7. Pu transcript b = *F GTPCH Isoform b = 1.75 (shorter version). Pu transcript c = GTPCH Isoform *F c = 1.75 (longer version), actually closer to 1.8. Pu transcript d = GTPCH *F Isoform d = 3.0 kb *F We have sequenced a full length cDNA corresponding to the 1.1 kb *F RNA. It has no strong similarities to products in data bases, so for the *F time being remains un-named. *F The 1 kb is a male-specific transcript, but we have no sequence *F information. We have no further data on any of the remaining transcripts *F except that none of them appear to share any exons with the GTPCH cDNAs. *F I hope this helps. If you have further questions, let me know. *F Janis O'Donnell *F From eleanor@gen.cam.ac.uk Mon Jun 02 16:15:43 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 2 Jun 1997 16:15:43 +0100 *F To: JODONNEL@biology.as.ua.edu *F Subject: Re: Help FlyBase - FBrf0049526 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 2 Jun 1997 16:16:10 +0100 *F Content-Length: 529 *F Good afternoon Janis! *F Thanks for all the information. *F I shall curate the data as a personal communication as there is loads of *F information that should be incorporated into FlyBase. *F I will have to generate 11 anon genes, but that is far better than the *F situation earlier today! *F For the 1 kb male-specific transcript, I shall give the gene the symbol *F Mst57Ba, meaning male specific transcript at 57B. This is a more informative *F symbol but if you prefer the anon symbol then please let me know. *F Thanks again, *F Eleanor Whitfield *F From JODONNEL@biology.as.ua.edu Mon Jun 02 18:49:22 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 2 Jun 1997 18:49:22 +0100 *F From: *F Organization: University of Alabama Dept. of Biol *F To: eleanor@gen.cam.ac.uk *F Date: Mon, 2 Jun 1997 11:59:38 CST6CDT *F Subject: Re: Help FlyBase - FBrf0049526 *F Priority: normal *F X-mailer: Pegasus Mail/Mac (v2.1.2) *F Content-Length: 433 *F Eleanor, Thanks for the information. Re: the male specific transcript, *F your designation is fine. More detailed descriptions of Pu transcripts a *F and b can be found in McLean et al., J. Biol. Chem. 268:27191-27197, *F 1993. We are refining sequence on trpt d, but hope to finally get a paper *F out on it and the c form by the end of the summer. I'll let you know if *F anything further comes out of the remaining sequencing. *F Janis # *U FBrf0093862 *a Paricio *b N. *t 1997.5.23 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Apr 25 13:17:52 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 25 Apr 1997 13:17:52 +0100 *F To: paricio@embl-heidelberg.de *F Subject: from FlyBase *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Fri, 25 Apr 1997 13:21:29 +0100 *F Content-Length: 1099 *F Dear Dr. Paricio, *F At the Drosophila research conference in Chicago I obtained some *F information from you about your new gene, muscleblind. The information was *F that the symbol for muscleblind should be mbd and that the cytological *F location of the gene is 54A1-54A3. *F Unfortunately I have discovered that the symbol mbd is already in use, for *F the gene mushroom body deranged, and I would therefore be grateful if you *F could suggest a new symbol for muscleblind. *F One possibility is mbl, which is not in use at the moment. *F Please could you check on FlyBase (http://flybase.bio.indiana.edu/) to *F check whether the symbol you decide on is already in use. *F I look forward to hearing from you, *F Gillian Millburn *F \------------------------------------------ *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \------------------------------------------ *F From paricio@mailserver.EMBL-Heidelberg.DE Fri May 23 14:14:06 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 23 May 1997 14:14:06 +0100 *F Date: Fri, 23 May 1997 15:17:46 +0100 *F Date-warning: Date header was inserted by Hera.EMBL-Heidelberg.DE *F From: paricio@mailserver.EMBL-Heidelberg.DE (Nuria Paricio) *F Subject: Re: from FlyBase *F X-Sender: paricio@pophost.embl-heidelberg.de *F To: Gillian Millburn (Genetics) *F MIME-version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-transfer-encoding: 7BIT *F Content-Length: 545 *F Dear Gillian, *F First of all, I am very sorry for the delay on my answer. Thank you for *F your message. Actually we knew about the mbd symbol just after the Chicago *F meeting. As you suggested, we have agreed to have mbl as symbol for our new *F gene, muscleblind. *F I hope we won t have any other problem in this respect. *F Best regards, *F Nuria Paricio *F Nuria Paricio *F EMBL, Developmental Biology Programm *F Meyerhofstrasse 1 *F Heidelberg D69117, Germany *F E-mail: paricio@embl-heidelberg.de *F Lab phone: (6221) 387388 *F Fax: (6221) 387516 # *U FBrf0093863 *a Roote *b J. *t 1995.5.13 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue May 13 20:00:39 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 13 May 1997 20:00:39 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 13 May 1997 20:05:25 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: new results *F Content-Length: 521 *F Aubrey, *F Aberration symbol Df(2L)el20 *F Full name Deficiency (2L) elbow *F Class of aberration Deficiency *F FlyBase id number FBab0001799 Graphic map *F Date 11 Feb 97 *F Breakpoints 34F4;??? 35C5 *F Discoverer(s) G. Johnson *F Mutagen or origin ethyl methanesulfonate *F Formalized genetic data l(2)34Fa << bk1 << wb << l(2)35Da << bk2 << *F sna *F should read rk << bk1 << l(2)34Fa << l(2)35Da << bk2 << sna *F Thanks, *F John # *U FBrf0093864 *a Roote *b J. *t 1997.6.3 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Jun 03 19:55:43 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 3 Jun 1997 19:55:43 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 3 Jun 1997 20:01:07 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Content-Length: 584 *F Aubrey, *F This is worth adding because it's the only ab that separates Dd, Df from De, Dg *F Dp(2;3)17 *F Formalized genetic data bk1 << esc << bk2 << bk3 << kuz << l(2)34Df *F << bk4 *F Formalized genetic data bk1 << esc << bk2 << bk3 << kuz << l(2)34Df, l(2)34Dd *F << bk4 l(2)34De, l(2)34Dg *F J *F ___________________________________________________________________ *F Michael Ashburner's lab Tel: 44 1223 333982 *F Department of Genetics Fax: 44 1223 333992 *F University of Cambridge email: j.roote@gen.cam.ac.uk *F Downing Street *F Cambridge *F CB2 3EH *F UK # *U FBrf0093865 *a Roote *b J. *t 1997.6.5 *T personal communication to FlyBase *u *F From: 'Kathy Matthews' *F Personal communication from: John Roote, University of Cambridge *F To: Bloomington Drosophila Stock Center *F Subject: Deficiency information for two recombinant inversion chromosomes *F Dated: 5 June 1997 *F *F Information communicated: *F *F In(2LR)DTD116[L]DTD24[R] is deficient for 26A4-6;26C1-2 *F In(2LR)DTD16[L]DTD42[R] is deficient for 23C;23E3-6 # *U FBrf0093866 *a Roote *b J. *t 1997.6.6 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Fri Jun 06 19:33:04 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Fri, 6 Jun 1997 19:33:04 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 6 Jun 1997 19:38:33 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: for fb updates *F Cc: ma11@gen.cam.ac.uk *F Content-Length: 2574 *F Aubrey, *F Here is a load of stuff that MA and I have just gone through: *F New alleles: *F rkKe1 PM allele from Kerbiniou P strain, discovered by Shelton *F 35BbAM10 .. call this 35Bb10 with AM10 as synonym. On Df(2L)b74c6, *F which was caffeine + gamma ray induced by Alexandrov. *F 35Bbk11524 from Kiss collection, mapped here *F l(2)35BfQL53 on BicCQL53, ref Schupbach and Weischaus 1991 Gen *F 129:1119, mapped here *F Su(H)E86 isolated and mapped by Cathy Shea (MIT), photoreceptor axon *F projection defects in optic lobe of 3rd instar larvae. (I don't know how it *F was induced.) *F Su(H)1356 ry+ P insert from Delattre et al. 1995 Genetics 141:1407 *F l(2)35BgPI2 PM allele from Shelton, using PI2 strain *F rdGJ3 from Glynnis Johnson (this lab), don't know how it was induced. *F gftGR18 on T(Y;2)TE35B-18 (gamma) *F esgflg: esgfleabag = fleabag, from Alan Shirras, Lancaster, PM induced *F from Athens 77Q strain *F l(2)35DfHL58 reference Schupbach and Weischaus 1986 Dev Biol 113:443, *F Table 5 Class 2Di - Tiny eggs - failure in transport. HL58/35Df- escapers *F are fs and have small bristles. *F tweDTD22 associated with T(2;4)DTD22 *F fzy8 on Scorv16 *F fs(2)35Ec no alleles, overlapping deletion phenotype *F These are leaky fs: RA5/el28, TE35B-2, TE35B-13, Scorv10, Scorv14, *F TE35D-24, A246, nBR120, pk-Drv2, pk-Drv3 *F These are completely fs: RA5/Scorv7, TE35BC-8, TE35D-3, TE35D-11, TE35D-17, *F TE35D-5, TE35D-12, nco4LD6R *F fs(2)35Ed no alleles, overlapping deletion phenotype *F leaky fs: RM5/Sco-7 *F fs: RM5/TE35D-6, TE35D-2, TE35D-14 *F Df(2L)TE35D-4 broken in stc; should be in FBrf0085996 *F needs an stc allele name - stcTE35D4 *F Cheers, *F John *F Date: Fri, 6 Jun 1997 12:15:46 +0000 *F To: Aubrey de Grey *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: update *F Content-Length: 507 *F Aubrey, *F Various things are coming out of our check on Adh region stuff in prep for *F Gerry's visit. Here's a start: *F kuzIK5. IK5 is an allele of kuz (=34Da), not 35Da *F l(2)k05224 recently mapped genetically and molecularly to Sos *F l(2)k06321 recently mapped molecularly to Sos *F l(2)k05605 recently mapped genetically and molecularly to l(2)35Dg *F rk84b1 induced at the same time as b84b1, gamma *F wb91g2 induced at the same time as osp91g2, EMS *F l(2)k05612 wb allele *F l(2)k00805 wb allele *F l(2)k13507 wb allele *F John *F From jr32@mole.bio.cam.ac.uk Mon Jun 09 15:04:50 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 9 Jun 1997 15:04:50 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 9 Jun 1997 15:10:26 +0000 *F To: Eleanor Whitfield (Genetics) *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: Re: for fb updates *F Content-Length: 1019 *F Ele. *F This is the story: *F TE35B-14 and TE35B-4 are 2 inversions derived from TE35B, so they *F both break in noc. -14 breaks in 42F, just proximal of nec (necrotic), -4 *F breaks in pk. The recombinant In(2LR)TE35B-14LTE35B-4R carries 3 *F copies of w+, 1 on the left break and 2 on the right and is deleted for *F nec. The stock has a zeste eye-colour in a zeste1 mutant background. We *F selected for red eyed derivatives of this and made stocks. One of these *F had lost the 2 copies from TE35B-4R and we called it SW250 (spontaneous *F white) - it had (has) a dominant prickle phenotype. We then foolishly *F made gamma-ray induced revertants of pkD. There are ~12 of these. I *F will send you what I know about them soon. *F J *F From jr32@mole.bio.cam.ac.uk Wed Jun 11 10:58:02 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 11 Jun 1997 10:58:02 +0100 *F X-Sender: jr32@mole.bio.cam.ac.uk (Unverified) *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 11 Jun 1997 11:00:39 +0000 *F To: Eleanor Whitfield (Genetics) *F From: jr32@mole.bio.cam.ac.uk (John Roote) *F Subject: pkDrv *F Content-Length: 1290 *F Name Cytology Genetics *F pkD1 In(2LR)TE35B-4LTE35B-250R noc pkD *F pkD1-rv1 In(2LR)+In(2R)43;49F noc pk-sple *F pkD1-rv2 In(2LR)+Df(2LR) Df(2LR)noc-fs(2)35Ec; pk-sple *F pkD1-rv3 In(2LR)+Df(2LR) Df(2LR)noc-fs(2)35Ec; pk-sple *F pkD1-rv4 In(2LR) noc pk *F pkD1-rv5 In(2LR) noc pk *F pkD1-rv6 In(2LR)+T(2;3)43;80 noc pk-sple *F pkD1-rv7 In(2LR)+In(2)het noc pk-sple *F pkD1-rv8 In(2LR)+Df(2LR) Df(2LR)noc-stc;pk-cn *F pkD1-rv9 In(2LR) noc pk-sple *F pkD1-rv10 In(2LR)+Df(2LR) Df(2LR)noc-l(2)35Da; pk-l(2)43Ef *F pkD1-rv11 In(2LR)SW251L+Df(2R) noc Df(2R)pk--scra *F pkD1-rv12 In(2LR)+Df(2R) noc Df(2R)pk--l(2)43Bb *F SW251 is a white eyed derivative of: *F In(2LR)TE35B-4L TE35B-SW250R *F (NB TE35B-4L not TE35B-14) # *U FBrf0093867 *a Tear *b G. *t 1997.6.13 *T personal communication to FlyBase *u *F From g.tear@ic.ac.uk Fri Jun 13 09:57:41 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 13 Jun 1997 09:57:41 +0100 *F X-Sender: gtear@biochem.bc.ic.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 13 Jun 1997 10:03:12 +0100 *F To: Michael Ashburner (Genetics) *F From: g.tear@ic.ac.uk (Guy Tear) *F Subject: Re: Help FlyBase please *F Content-Length: 939 *F Michael, *F Robo is in the process of being written up and will hopefully have a *F reference by the end of the year. *F Its location has been identified by the robo mutation being uncovered by *F the deficiencies Df(2R)X58-5, 58A3/B2 - 59A and Df(2R)X58-12 58D1,2 - *F 59A (Kerrebrock et al Cell 83 247), complemented by Df(2R)59AB 59A1/3 - *F 59B1/2 (Knoblich & Lehner, 1993, EMBO 12 65) and mapping distal to plexus *F by recombination. (Df(2R)59AB fails to complement both Df(2R)X58-5 and *F Df(2R)X58-12). *F All the best, *F Guy. *F ============================================================================= *F Dr. Guy Tear Office: (44) 171-594 5305 *F Department of Biochemistry Lab: (44) 171-594 5306 *F Imperial College FAX: (44) 171-594 5207 *F Exhibition Road *F London SW7 2AZ *F England *F ============================================================================== # *U FBrf0093868 *a Mount *b S. *t 1997.4.17 *T personal communication to FlyBase *u *F Archived *F consequent communications: *F From eleanor@gen.cam.ac.uk Fri Apr 25 15:31:19 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 25 Apr 1997 15:31:19 +0100 *F To: sm193@umail.umd.edu *F Subject: FlyBase Update form - E(Dfd) v B52 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 25 Apr 1997 15:31:53 +0100 *F Content-Length: 857 *F Hi Steve, *F I am curating information from the FlyBase update forms, you submitted *F information concerning B52, known as E(Dfd) in FlyBase. *F I have looked into this case in great detail and found that the symbol B52 *F does win over the symbol SRp55, given by the Roth group, by precedence. *F But then in the 1994 abstract: *F FBrf0068180 == Mount and Peng, 1994, A. Conf. Dros. Res. 35 : 247 *F you state *F 'We have shown that the abundant Drosophila SR protein B52/dSRp55 is encoded *F by the Enhancer-of-Deformed (E(Dfd)) gene (3-52; 87F).' *F And in the abstract you go on to call the protein B52/dSRp55 and the gene *F E(Dfd). *F Do you now wish the gene symbol to be B52, overruling what you stated in *F the abstract? *F Please confirm this as I will not act on the information provided by you in *F the update form until I have heard from you. *F Regards, *F Eleanor Whitfield *F FlyBase *F From smount@wam.umd.edu Fri Apr 25 17:59:24 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 25 Apr 1997 17:59:24 +0100 *F X-Authentication-Warning: rac2.wam.umd.edu: smount owned process doing -bs *F Date: Fri, 25 Apr 1997 13:02:20 -0400 (EDT) *F From: Stephen M Mount *F To: Eleanor Whitfield *F Subject: Re: FlyBase Update form - E(Dfd) v B52 *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 2811 *F > Hi Steve, *F Hi. *F > I am curating information from the FlyBase update forms, you submitted *F > information concerning B52, known as E(Dfd) in FlyBase. *F > *F > I have looked into this case in great detail and found that the symbol B52 *F > does win over the symbol SRp55, given by the Roth group, by precedence. *F There is also the matter of clarity - SRp55 in mammals is clearly *F homologous, but may not be a true homolog, as SRp40 and SRp75 are about *F equally similar in the RNA-binding domains. *F > But then in the 1994 abstract: *F > FBrf0068180 == Mount and Peng, 1994, A. Conf. Dros. Res. 35 : 247 *F > you state *F > 'We have shown that the abundant Drosophila SR protein B52/dSRp55 is encoded *F > by the Enhancer-of-Deformed (E(Dfd)) gene (3-52; 87F).' *F > And in the abstract you go on to call the protein B52/dSRp55 and the gene *F > E(Dfd). *F > *F > Do you now wish the gene symbol to be B52, overruling what you stated in *F > the abstract? *F Yes, the point is that Ring and Lis were the first to publish a mutation *F in the gene and examine it's phenotype. Therefore, we followed suit when *F we published a little while later. Although the abstract was clear about *F our intent to call the gene E(Dfd), we changed our mind while writing the *F paper because we thought that it would be best if all publications used a *F common gene name, and did not consider the abstract to have been *F precedent-setting (since it was not a refereed publication). The *F motivation behind 'E(Dfd)' was that it was a bit more interesting than *F 'B52' (which was Michael Ashburner's comment last week) and made *F reference to a phenotype, but we decided in favor of consistent *F nomenclature in publications. 'E(Dfd)' is used as a name solely in that *F abstract (and possibly one other), so changing the flybase name to B52 *F makes the most sense. I am confident that John Lis would agree. *F > Please confirm this as I will not act on the information provided by you in *F > the update form until I have heard from you. *F Now you have. I hope this has been clear. *F Thank you so much for your considerable effort. *F Steve Mount *F P.S. On a related matter: The work of several laboratories (Kelley, Haynes *F and my own) has established a gene order for sqd, Hrb87, and B52, which *F are tightly clustered (with Nts and tsr - contact Sue Haynes for details). *F The molecular map is shown in Peng and Mount. I also have pretty good *F meiotic recombination data in that paper. I mention these things because *F it might be possible to update *F http://www.ebi.ac.uk:7081/genes/by-map/3-049.-_to_3-054.-... *F to include this information. *F \########################################### *F Stephen M. Mount *F Cell Biology & Molecular Genetics *F Room 3202 Zoology Bldg. *F University of Maryland *F College Park, MD 20742-4415 *F Phone 301-405-6934 *F FAX 301-314-9358 *F email sm193@umail.umd.edu *F From eleanor@gen.cam.ac.uk Mon Apr 28 09:07:05 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 28 Apr 1997 09:07:05 +0100 *F To: smount@wam.umd.edu *F Subject: Re: FlyBase Update form - E(Dfd) v B52 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 28 Apr 1997 09:07:27 +0100 *F Content-Length: 1015 *F Hi, *F Thank you for your prompt reply. *F With the information you have provided FlyBase shall change the symbol from *F E(Dfd) to B52. *F >P.S. On a related matter: The work of several laboratories (Kelley, Haynes *F >and my own) has established a gene order for sqd, Hrb87, and B52. *F We have this information from curation of FBrf0084258 (Peng and Mount, *F 1995, Molec. Cell. Biol. 15(11): 6273--6282), unfortunately this data is *F not publically available yet but it should be soon. *F The field states: *F Gene order: sqd- Hrb87F- B52+ *F Let me know if this is incorrect or if you have any further information. *F >I also have pretty good *F >meiotic recombination data in that paper. *F If you could give me this data it could included as part of a personal *F communication from you to FlyBase (along with the information regarding B52 *F v E(Dfd)). From looking at the paper you discuss the recombination *F analysis but do not report the location of B52. *F >Thank you so much for your considerable effort. *F All part of my job! *F Eleanor Whitfield *F From smount@wam.umd.edu Wed May 07 04:40:27 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 7 May 1997 04:40:27 +0100 *F X-Authentication-Warning: rac9.wam.umd.edu: smount owned process doing -bs *F Date: Tue, 6 May 1997 23:43:46 -0400 (EDT) *F From: Stephen M Mount *F To: Eleanor Whitfield *F Subject: Re: FlyBase Update form - E(Dfd) v B52 *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 152 *F Eleanor *F I am out of town at the moment. The gene order is correct. I will look up *F my recombination data and forward it to you next week. *F Steve Mount *F From smount@wam.umd.edu Tue May 27 20:13:11 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 27 May 1997 20:13:11 +0100 *F X-Authentication-Warning: rac6.wam.umd.edu: smount owned process doing -bs *F Date: Tue, 27 May 1997 15:16:47 -0400 (EDT) *F From: Stephen M Mount *F To: Eleanor Whitfield *F Subject: Re: FlyBase Update form - E(Dfd) v B52 *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1312 *F Eleanor *F Thanks for nagging me. I have been impossibly busy with the things that *F accumulate at the end of an academic semester (many things involving *F students are to be done 'by the end of the term'). *F In any case, I have checked my original notes. In a cross of *F Df(3R)urd / TM3, Sb males X B52ED / ry506 sqdix50 cv-c sbd females *F a total of 13,980 Stubble progeny were counted. Because both B52ED and *F sqdix50 are lethal over Df(3R)urd, most of the progeny would carry TM3, *F Sb. The exceptions would be recombinants. *F A single 'Stubble +' fly was observed among these 13,980 Stubble progeny. *F It was verified as carrying Df(3R)urd in trans to ry+ sqd+ B52+ cv-c *F sbd. Thus, this one recombinant orients the molecular and genetic maps as *F shown in our MCB paper. *F While I carried out a number of other recombination experiments, and *F obtained recombinants between B52 and close markers (B52 and cv-c), I see *F that my numbers are not as good as I had thought, so I have no additional *F data to report. *F Steve Mount *F \########################################### *F Stephen M. Mount *F Depts. of Cell Biology & Molecular Genetics and Biology *F Room 3202 Zoology *F University of Maryland *F College Park, MD 20742-4415 *F Phone 301-405-6934 *F FAX 301-314-9358 *F email sm193@umail.umd.edu # *U FBrf0093869 *a Davis *b I. *t 1996.11.22 *T personal communication to FlyBase *u *F Archived. # *U FBrf0093870 *a Benes *b V. *t 1997.7.25 *T personal communication to FlyBase *u *F >From Vladimir.Benes@EMBL-Heidelberg.de Tue Jul 15 12:07:22 1997 *F Date: Tue, 15 Jul 1997 18:05:38 +0200 (MDT) *F X-Sender: benes@popserver.embl-heidelberg.de *F X-Mailer: Windows Eudora Version 1.4.4 *F Mime-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F From: Vladimir.Benes@EMBL-Heidelberg.de (Vladimir Benes) *F Subject: gap in sequence of pP(PZ) vector *F *F Dear Madam, dear Sir, *F in the frame of collaboration with the Marek Mlodzik's group here in EMBL I *F filled the gap in the sequence of pP(PZ) vector between bases 3901 and 4543. *F I would like to ask you about the best way to send the updated sequence to *F you or to a place you will indicate. Please let me know. *F With best regards. *F Yours, *F Vladimir Benes *F *F >From crosby@morgan Wed Jul 23 16:37:47 1997 *F Date: Wed, 23 Jul 1997 16:37:19 -0400 *F From: crosby@morgan (Madeline Crosby) *F To: Vladimir.Benes@EMBL-Heidelberg.de *F Subject: Re: sequence to fill the gap between pos. 3900 and 4550 (approx.) in the vector pP(pZ) and related vectors *F Cc: crosby@morgan *F X-Sun-Charset: US-ASCII *F Content-Length: 1246 *F *F *F Vladimir, *F *F Thanks so much for the sequence data. Definitely fills the gap for these *F vectors. *F *F This sequence is derived from Hsp70Ab and adds significantly to the *F publically-available sequence for that gene (AC# J01103). That sequence *F record ends at position 3901 in your sequence. In your data, the *F Hsp70Ab-derived sequence ends at 4543, which corresponds to the BglII site *F 3' of the Hsp70Ab gene (no longer a BglII site in pP{PZ} since was ligated *F to a BamHI site). Thus 3902-4543 is new 3' sequence for Hsp70Ab. You *F might consider submitting it to GenBank or EMBL as such. (See Moran et al., *F 1979, Cell 17:1-8 -- can verify that the appropriate restriction sites *F do indeed show up where they should in your sequence.) *F *F Carl Thummel's lab has actually sequenced a smaller portion of this 3' *F Hsp70Ab region as well; it is in AC# U59056 coordinates 225-38 (complement). *F Their sequence agrees with your insertion of a T at 3380. However, there *F are 3 additional insertions in your sequence versus theirs (3911, 3921, 3931). *F Are you relatively certain of these regions? They find a T at position 3992. *F *F Thanks for the input. Please let me know if you decide to submit anything *F to GenBank/EMBL. *F *F *F Sincerely, *F *F Lynn Crosby *F FlyBase *F *F >From Vladimir.Benes@EMBL-Heidelberg.de Thu Jul 24 05:22:00 1997 *F Date: Thu, 24 Jul 1997 11:20:23 +0200 (MDT) *F X-Sender: benes@popserver.embl-heidelberg.de *F X-Mailer: Windows Eudora Version 1.4.4 *F Mime-Version: 1.0 *F To: crosby@morgan.harvard.edu (Madeline Crosby) *F From: Vladimir.Benes@EMBL-Heidelberg.de (Vladimir Benes) *F Subject: Re: sequence to fill the gap between pos. 3900 and 4550 (approx.) in the vector pP(pZ) and related vectors *F *F Dear Madeline, *F I checked once more sequence in the positions you indicated in your mail. I *F am certain that the bases are correct. However, I omitted to correct the *F ambiguity at position 3992; it's a clear T (actually, there are three *F sequences over this area). Sorry for that. *F Surprisingly perhaps the missing part (most of it) has been already *F sequenced by somebody else (unknowingly?) and is in the database under accno *F U03466. *F Do you think it's really necessary to submit this piece into database? It *F really is rather by-product than the result of aimed effort. I guess it *F serves its purpose much better if it is in your database. Please let me know. *F With best regards. *F Yours, *F Vladimir *F *F >From Vladimir.Benes@EMBL-Heidelberg.de Mon Aug 4 07:13:27 1997 *F Date: Mon, 4 Aug 1997 13:11:42 +0200 (MDT) *F X-Sender: benes@popserver.embl-heidelberg.de *F X-Mailer: Windows Eudora Version 1.4.4 *F Mime-Version: 1.0 *F To: crosby@morgan.harvard.edu (Madeline Crosby) *F From: Vladimir.Benes@EMBL-Heidelberg.de (Vladimir Benes) *F Subject: Re: EMBL submission DS:30677 *F *F Dear Madeline, *F thank you very much for hadling the database matter so smoothly. I am *F sending you Acc# of the entry for the reference: Y14393. *F With best regards and wishes of nice summer. *F Yours, *F Vladimir *F *F *F >From Vladimir.Benes@EMBL-Heidelberg.de Wed Aug 6 12:03:39 1997 *F Date: Wed, 6 Aug 1997 18:01:53 +0200 (MDT) *F X-Sender: benes@popserver.embl-heidelberg.de *F X-Mailer: Windows Eudora Version 1.4.4 *F Mime-Version: 1.0 *F To: crosby@morgan.harvard.edu (Madeline Crosby) *F From: Vladimir.Benes@EMBL-Heidelberg.de (Vladimir Benes) *F Subject: another issue *F *F Dear Madeline, *F I have this thing I would like to discuss with you and have your opinion on *F it, eventually. As you know from previous mails there was some work done on *F pZ vector here in EMBL. Now I returned to the sequences in order to look at *F them if I could fill some more gaps. I loaded one more sequence to the *F project that was not there before. It's matching your flybase sequence of pZ *F but the sequences between two short gaps at positions 5884 and 6064 are *F different. Well not really! I've got it. That segment of your sequence is *F inverted when compared with how it really is in the vector. I inverted *F "your" segment and the sequences are identical! *F I am sending you the part of pZ between 5850 and 6100. It covers both gaps *F and corrects the orientation of the segment: *F *F 5851 CCCTGAGTGC TTGCGGCAGC GTGAAGCTAA TTCATGGTTA AATTTTTGTT *F 5901 AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT *F 5951 AAATCAAAAG AATAGCCCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA *F 6001 CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA *F 6051 CCGTCTATCA GGGCGATGGC CGGATCAGCT TATGCGGTGT GAAATACCGC *F *F With best regards and all good wishes. *F Yours, *F Vladimir *F *F P.S. Sorry for that narrative part at the beginning of this mail but I *F really sorted that out during writing you this mail. *F *F *F >From crosby@morgan Wed Aug 6 13:14:47 1997 *F Date: Wed, 6 Aug 1997 13:14:16 -0400 *F From: crosby@morgan (Madeline Crosby) *F To: Vladimir.Benes@EMBL-Heidelberg.de *F Subject: Re: another issue *F Cc: crosby@morgan *F X-Sun-Charset: US-ASCII *F Content-Length: 621 *F *F *F Vladimir, *F *F This is terrific! You will note at the top of the page of the P{PZ} *F FlyBase sequence, there is a caution that the orientation of this *F segment is not known and that the orientation presented is arbitrary. *F However, that will soon be past tense -- thank you, again. *F *F There is one remaining hole, if you should get around to it, at *F 6926..6931. *F *F We will want to save your message as a personal communication, but *F you will be relieved to know that we do store small stretches of *F "junction" sequences at FlyBase. So, no need to submit this bit *F to EMBL. *F *F Thanks being so generous with your findings. *F *F *F --Lynn *F *F *F >From Vladimir.Benes@EMBL-Heidelberg.de Wed Aug 6 13:54:00 1997 *F Date: Wed, 6 Aug 1997 19:52:09 +0200 (MDT) *F X-Sender: benes@popserver.embl-heidelberg.de *F X-Mailer: Windows Eudora Version 1.4.4 *F Mime-Version: 1.0 *F To: crosby@morgan.harvard.edu (Madeline Crosby) *F From: Vladimir.Benes@EMBL-Heidelberg.de (Vladimir Benes) *F Subject: Re: another issue *F *F Dear Madeline, *F that's exactly what I discovered when I checked on the web page with pZ *F vector (I mean the reference to arbitrary orientation of the segment). I am *F really glad it is not necessary to submit this piece to the database *F although it went quite smoothly with your help in the case of the previous *F sequence... *F Well, with the remaining gaps (I actually see one more at position 11846 and *F am not sure I'll ever get to sequence in that area) it depends whether I'll *F get rescue that doesn't start with XbaI site. I'll certainly keep you posted *F when there is a sequence closing this gap. *F With best regards. *F Yours, *F Vladimir *F *F >From crosby@morgan Wed Jul 16 11:29:40 1997 *F Date: Wed, 16 Jul 1997 11:29:10 -0400 *F From: crosby@morgan (Madeline Crosby) *F To: Vladimir.Benes@EMBL-Heidelberg.de *F Subject: Re: gap in sequence of pP(PZ) vector *F Cc: flybase-help@morgan *F X-Sun-Charset: US-ASCII *F Content-Length: 1261 *F *F *F Dear Vladimir, *F *F >in the frame of collaboration with the Marek Mlodzik's group here in EMBL I *F >filled the gap in the sequence of pP(PZ) vector between bases 3901 and 4543. *F >I would like to ask you about the best way to send the updated sequence to *F >you or to a place you will indicate. *F *F Absolutely terrific! This is the last major gap in the P{PZ} sequence *F (also fills major holes in P{FZ} and P{HZ}). *F *F You can simply e-mail the sequence to flybase-updates@morgan.harvard.edu. *F Any format, upper or lower case, is OK. Please include significant *F overlap with known sequence, so I can accurately insert it into the compiled *F sequence. *F *F If you picked up any discrepancies between your data and the compiled *F sequence, we would also be interested to know where and how significant *F the discrepancies are. There are other regions of uncertainty, especially *F in the sequence derived from pHSS7; if you any further information about *F any of these regions, we would be very interested. *F *F We will record and save your information as a personal communication from *F you to FlyBase, and you will be cited as the source of this sequence. *F *F Thanks for taking the time to inform us and for allowing other users *F to benefit from your efforts. *F *F *F Sincerely, *F *F Lynn Crosby *F FlyBase *F *F >From Vladimir.Benes@EMBL-Heidelberg.de Mon Jul 21 11:17:34 1997 *F Date: Mon, 21 Jul 1997 17:15:53 +0200 (MDT) *F X-Sender: benes@popserver.embl-heidelberg.de *F X-Mailer: Windows Eudora Version 1.4.4 *F Mime-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F From: Vladimir.Benes@EMBL-Heidelberg.de (Vladimir Benes) *F Subject: sequence to fill the gap between pos. 3900 and 4550 (approx.) in the vector pP(pZ) and related vectors *F *F 3851 GAAATTTAAA GTCAACTTGT CATTTAATGT CTTGTAGACT TTTGAAAGTC *F 3901 TTACGATACA ATTAGTATCT AATATACATG GGTTCATTCT ACATTCTATA *F 3951 TTAGTGATGA TTTCTTTAGC TAGTAATACA TTTTAATTAT AWTCGGCTTT *F 4001 GATGATTTTC TGATTTTTTC CGAACGGATT TTCGTAGACC CTTTCGATCT *F 4051 CATAATGGCT CATTTTATTG CGATGGACGG TCAGGAGAGC TCCACTTTTG *F 4101 AATTTCTGTT CGCAGACACC GCATTTGTAG CACATAGCCG GGACATCCGG *F 4151 TTTGGGGAGA TTTTCCAGTC TCTGTTGCAA TTGGTTTTCG GGAATGCGTT *F 4201 GCAGGCGCAT ACGCTCTATA TCCTCCGAAC GGCGCTGGTT GACCCTAGCA *F 4251 TTTACATAAG GATCAGCAGC AAAATTTGCC TCTGCTTCAT TGCCCGGAAT *F 4301 CACAGCAATC AGATGTCCCT TTCGGTTACG ATGGATATTC AGGTGCGAAC *F 4351 CGCACACAAA GCTCTCGCCG CACACTCCAC ACTGATATGG TCGCTCGCCC *F 4401 GTGTGGCGCC GCATATGGAT CTTAAGGTCG TTGGACTGCA CAAAGCTCTT *F 4451 GCTGCACATT TTGCAGGAGT ACGGCCTTTG ACCCGTGTGC AATCGCATGT *F 4501 GTCGCGCCAG CTTGTTCTGC GAAATAAACT TCTTGGAGCA GATCCGCGGC *F 4551 CGCCCGGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT *F *F Dear Madeline, *F There is 50 bases overlap on each side with the sequence of the vector *F pP(pZ). There is an additional T in pos. 3880 which is missing in the *F sequence of this vector in the database. *F Please let me know if the format is sufficient for complementing the gap. *F With best regards. *F Yours, *F Vladimir *F *F *F *F *F # *U FBrf0093872 *T personal communication to FlyBase *u *F Archived # *U FBrf0094082 *a Aza-Blanc *b P. *t 1997.8.14 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon Jul 14 11:21:23 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 14 Jul 1997 11:21:23 +0100 *F To: tomk@cgl.ucsf.edu *F Subject: Help FlyBase - P{Ub+>GAL4} *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 14 Jul 1997 11:25:53 +0100 *F Content-Length: 541 *F Hi, *F I am curating a paper of yours for FlyBase: *F Aza-Blanc et al., 1997, Cell 89(7): 1043-1053 *F in which you use the construct P{Ub+>GAL4}. *F We do not have this construct in FlyBase so could you please provide *F minimal details of its construction and references if you have them. *F Please include details of: *F Which gene the promoter fragment is taken from *F Length and ends of promoter fragment *F Length and ends of yellow fragment, cNDA or genomic DNA *F Origin of the stop signal within the FRT cassette. *F Many thanks, *F Eleanor Whitfield *F FlyBase *F From azablanc@cgl.ucsf.EDU Thu Aug 14 22:41:17 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Thu, 14 Aug 1997 22:41:17 +0100 *F Date: Thu, 14 Aug 1997 14:46:29 -0700 (PDT) *F From: Pedro Aza-blanc *F X-Sender: azablanc@socrates.ucsf.edu *F To: eleanor@gen.cam.ac.uk *F Subject: pUb>y+>GAL4 *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1076 *F regarding the plasmid pUb>y+>GAL4 (Aza-Blanc et al., 1997): *F made on W8 vector *F contains: poly-ubiquitin promoter; frt-y+-frt-ires cassette; GAL4 gene; *F tubulin3' utr *F Promoter used: poly-ubiquitin promoter, taken from pCa4GFPh27 (built by *F Ilan Davis). A Eco RI site was introduced by PCR substituting the first *F codon, and then the promoter was cut out with EcoRI. Approx. size: 2000bp *F y+-frt cassette: taken from pKB700 (from K. Basler). contains an approx. *F 7500bp genomic fragment including promoter and open reading frame of y+ *F flanked by FRT sequences (approx. 75bp each) It also contains an IRES *F (internal ribosomal entry site) after the second FRT. the cassette was *F taken out from pkb700 by cutting with KpnI (other KpnI sites within the y+ *F region have been destroyed, see pkb700) *F GAL4 was obtained from pGAWB, by cutting with NotI/partial HindIII;(there *F is another HindIII site right after the 5' NotI site, so GAL4 can be *F excised out with HindIII alone. *F Tubulin 3' UTR: from pKB700, by excision with EcoRI/XbaI; Approximate *F size: 800bp *F Pedro Aza-Blanc # *U FBrf0094188 *a Bloomington Drosophila Stock Center *b ?. *t 1997.7.17 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Cass lethals in 98D *F Date: 17 July 1997 *F *F Background: Cynthia Cass describes the recovery of lethals in 98D, 7 of which we now at Bloomington (see accompanying FAX from Cass). She named them M#, *F for example, M313. These have been through 3 rounds of renaming, the latest being l(3)98D-Ea[1], for example (complete list below for the 7 we have). *F *F Cass Name BG Name 1 BG Name 2 BG Name 3 *F M313 l(3)M3-13 l(3)98Da l(3)98D-Ea[1] *F M35 l(3)M3-5 l(3)98Db l(3)98D-Eb[1] *F M318 l(3)M3-18 l(3)98Dc l(3)98D-Ec[1] *F M33 l(3)M3-3 l(3)98Dd l(3)98D-Ed[1] *F M15 l(3)M1-5 l(3)98De l(3)98D-Ee[1] *F M37 l(3)M3-7 l(3)98Df l(3)98D-Ef[1] *F M312 l(3)M3-12 l(3)98Dg l(3)98D-Eg[1] # *U FBrf0094392 *a Cass *b C. *t 1997.7.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0094426 *a Chihara *b C. *t 1997.7.30 *T personal communication to FlyBase *u *F From chihara@usfca.edu Wed Jul 30 21:21:09 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 30 Jul 1997 21:21:09 +0100 *F X-Sender: chihara@pop.usfca.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 29 Jul 1997 13:31:28 -0800 *F To: Michael Ashburner (Genetics) *F From: chihara@usfca.edu (Carol Chihara) *F Subject: Re: Lcp genes again *F Cc: lmr@zoology.washington.edu *F Content-Length: 3535 *F *F Michael, *F *F ... *F *F LCP7 is listed as having been mapped to the 3-11 position. NOT SO. We *F don't know where 7 maps - and we do know now, that it is NOT at 65A. *F *F FlyBase lists LCP9, 10 and rho as the same protein. Not so. Rho was *F renamed as 10 by Flybase, that's O.K. But there is a protein at the position *F of LCP9 which was not discussed, although it can be seen on some of the *F figures in the 1985 paper. It is named as LCP9 in a second submitted paper *F with Lynn, and is NOT found in the 65A cluster. *F *F ... *F *F Carol # *U FBrf0094427 *a Chihara *b C. *t 1997.7.31 *T personal communication to FlyBase *u *F From chihara@usfca.edu Thu Jul 31 19:01:55 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 31 Jul 1997 19:01:55 +0100 *F X-Sender: chihara@pop.admin.usfca.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 31 Jul 1997 11:15:26 -0700 *F To: Michael Ashburner (Genetics) *F From: Carol Chihara *F Subject: Re: Lcp genes again *F Cc: lmr@zoology.washington.edu *F Content-Length: 2681 *F *F Michael, *F *F Sorry it was confusing, it is. *F *F SEE BELOW, the list helps. *F *F >This is the list of genes that I thought we had agreed: \*a is the FlyBase *F >gene symbol, \*i the synonym, \*g GenBank records and \*m TREMBL records *F >(protein sequences, derived from Genbank). Is this not correct. Duplicate *F >genes get different names ! Are 65Ab1 and 65Ab2 duplicate genes or alleles *F >of the same gene ??? Or are they what FlyBase has now as Acp65Ab and Lcp65Ab *F >(ie one an adult cuticle protein, other larval). *F > *F >I realise the problems about the alleles and if they cannot be assigned *F >we will add to one of the (new) genes arbitrarrily with notes that *F >some may belong to its duplicate sibling. *F *F O.K. *F *F 7 and 9 corrections O.K. *F *F I think it goes like this - adapted from the list you sent: *F *F *F O.K. *F >*a Acp65Aa *F >d adult-cuticle-protein *F >*g U84750; g1857602 *F >*m TREMBL/P91941 *F >*i DCP5 *F >*c 65A5-65A6 *F *F This is not correct as follows: [Corrections in square brackets.]errors *F surrounded by _underline_ *F We have to check the database \*s with Lynn, but Acp65Aa as above is the *F only adult cuticle protein gene in the cluster. *F *F >*a _Acp65Ab_ [should be Lcp65Ab1] *F >*d _Adult_ [Larval]-cuticle-protein *F >*y FBgn0002536 *F >*g U84746; g1857595;[U81550] *F >*m TREMBL/P92192 *F >*i DCP[3]-alpha *F >*i Lcp5 *F >*c 65A5-65A6 *F *F *F *F Next is O.K. *F ># *F >*a Lcp65Aa *F >*d larval-cuticle-protein *F >*g U84749; g1857600 *F >*m TREMBL/P91940 *F >*i DCP4 *F >*c 65A5-65A6 *F *F NOT O.K. *F ># *F >*a Lcp65Ab - [this should be Lcp65Ab2] This is a duplicate of Ab1 above *F >*d larval-cuticle-protein *F >*y FBgn0002536 *F >*g U84747; g1857597 *F >*m TREMBL/P92192 *F >*i DCP[3]-beta *F >*i Lcp5 *F >*c 65A5-65A6 *F *F The rest are O.K. *F ># *F >*a Lcp65Ac *F >*d larval-cuticle-protein *F >*g U84745; g1857593 *F >*g U84755; g1857612 *F >*m TREMBL/P92181 *F >*i DCP2 *F >*c 65A5-65A6 *F ># *F >*a Lcp65Ad *F >*d larval-cuticle-protein *F >*g U83247; g1857495 *F >*m TREMBL/P91939 *F >*i DCP1 *F >*c 65A5-65A6 *F ># *F >*a Lcp65Ae *F >*d larval-cuticle-protein *F >*g U84751; g1857604 *F >*m TREMBL/P91942 *F >*i DCP6 *F >*c 65A5-65A6 *F ># *F >*a Lcp65Af *F >*d larval-cuticle-protein *F >*g U84752; g1857606 *F >*g U84758; g1857618 *F >*m TREMBL/P92194 *F >*i DCP7 *F >*c 65A5-65A6 *F ># *F >*a Lcp65Ag1 *F >*d larval-cuticle-protein *F >*g U84753; g1857608 *F >*m TREMBL/P92201 *F >*i DCP8-alpha *F >*c 65A5-65A6 *F >*i Lcp8 *F ># *F >*a Lcp65Ag2 *F >*d larval-cuticle-protein *F >*g U84754; g1857610 *F >*m TREMBL/P92201 *F >*i DCP8-beta *F >*c 65A5-65A6 *F >*i Lcp8 *F ># *F *F Hope this clarifies it : *F *F The genes in the cluster are : *F From left to right; *F *F Acp65Aa *F Lcp65Aa *F Lcp65Ab1 (LCP5) *F Lcp65Aa (psuedo) *F Lcp65Ab2 (LCP5) *F Lcp65Ac *F Lcp65Ad *F Spacer *F Lcp65Ae *F Lcp65Af *F Lcp65Ag1 (LCP8) *F Lcp65Ag2 (LCP8) *F *F Carol *F *F Dr. Carol Chihara *F Department of Biology *F University of San Francisco *F 2130 Fulton St. *F S.F.CA 94117-1080 # *U FBrf0094478 *a Coen *b D. *t 1997.8.14 *T personal communication to FlyBase *u *F From dario.coen@emex.u-psud.fr Thu Aug 14 16:13:14 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 14 Aug 1997 16:13:14 +0100 *F X-Sender: coen@psisun.u-psud.fr *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 14 Aug 1997 17:25:01 +0200 *F To: Rachel Drysdale (Genetics) *F From: Dario Coen *F Subject: Re: Helping FlyBase crep and oap *F Content-Length: 1855 *F Dear Dr Drysdale, *F crep is indeed allelic to mirror, as well as D1, D3,Sail1and *F Sail2. We therefore call the various alleles mirrD1, mirrSai1 or *F mirrcre1, mirrcre2 etc... *F For oap, we have separated by recombination the lethality from the *F P insertion which is in araucam. We keep the name oap for the lethal which *F is located between caup amd mirror. We are trying to obtain other alleles *F of it. *F Very cordially *F Dario *F Dario COEN *F Embryologie Moleculaire et Experimentale *F Universite Paris Sud - Batiment 445 *F 91405 Orsay cedex FRANCE *F tel: 01 69 15 68 88 *F fax: 01 69 15 68 02 *F e-mail: dario.coen@emex.u-psud.fr # *U FBrf0094541 *a Crowley *b T. *t 1997.7.14 *T personal communication to FlyBase *u *F From tc45@columbia.edu Mon Jul 14 22:48:04 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 14 Jul 1997 22:48:04 +0100 *F Date: Mon, 14 Jul 1997 17:52:09 -0400 (EDT) *F From: Thomas Crowley *F Subject: Trf cluster *F X-Sender: tc45@pop.columbia.edu *F To: flybase-updates@morgan.harvard.edu *F MIME-version: 1.0 *F X-Mailer: Windows Eudora Light Version 1.5.4 (16) *F Content-Type: text/plain; charset='us-ascii' *F Content-transfer-encoding: 7BIT *F Content-Length: 2878 *F I would like to submit the following information regarding 2 genes of the *F Trf cluster on the left arm of the 2nd chromosome at polytene band 28DE. An *F entry for the Trf gene already exists in Flybase, so the info I am sending *F concerns the 2 flanking genes. *F The first Trf allele, PlacWTrf, arose in the enhancer-trap screen *F carried out in the Jan lab at UCSF in the late 80's, and is characterized by *F reporter gene expression in the embryonic CNS and spermatocytes in the adult *F testis. Two phenotypes are associated with this mutation: leg-shaking in *F ether-anesthetized adults and male-sterility. The 3 genes of the cluster lie *F on one side of the transposon insertion, with Trf in the middle. The *F insertion site is used as a point of reference for this locus, thus *F providing the name Trf-proximal (Trfp) for the gene between the insertion *F site and Trf. The gene most distal from the insertion site, Mcr, is named *F based on protein sequence similarity. *F Trfp: *F Transcription of this gene has only been observed in the *F spermatocytes of the adult testis. The cDNAs described below were obtained *F from a testis library. Transcript is at least 950 nt based on the length of *F the longest characterized cDNA, and an open reading frame (ORF) of 252 *F codons is found reading one strand of the sequence which is significantly *F longer than any ORF on the other strand. The hypothetical translation of *F this sequence yields an amino acid sequence, referred to as TRFP, with no *F similarity to any in the protein databases. *F Accession numbers *F nucleotide: EMBL y10975 *F protein: SPTREMBL P91641 *F Macroglobulin-related protein (Mcr): *F Transcribed in spermatocytes and in the embryonic PNS, cDNAs for *F this gene have been obtained from both embryo and testis libraries, the *F longest of which is 5.1 kb. The transcript detected on an embryo Northern is *F 8 kb, and the ORF of 1450 codons in the sequence of the longest cDNA extends *F to the 5' end of the clone, indicating that there is likely to be more *F protein sequence than that available in the characterized cDNAs. The *F predicted amino acid sequence, referred to as MRP, is similar to the *F mammalian serum proteinase-inhibitor, alpha-2-Macroglobulin, and contains a *F complement-type repeat which is found in several of the complement proteins *F of the mammalian immune system and the LDL receptor. *F Nucleotide accession number: EMBL y11116 *F Based on the finding of the ORFs described above, both Trfp and Mcr *F are transcribed towards the insertion site, while Trf is transcribed away. *F Excision of the transposon along with 0.5 kb of flanking chromosomal *F sequence is a larval-lethal mutation. *F Reference: *F Crowley TE, Hoey T, Liu JK, Jan YN, Jan L, Tjian R (1993) Nature 361:557-561 *F Thanks, *F Tom Crowley *F From eleanor@gen.cam.ac.uk Tue Jul 15 08:47:15 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 08:47:15 +0100 *F Date: Tue, 15 Jul 1997 08:51:16 +0100 *F From: Eleanor Whitfield (Genetics) *F Subject: Re: Trf cluster *F To: tc45@columbia.edu *F Cc: flybase-updates@morgan.harvard.edu *F Content-transfer-encoding: 7BIT *F Content-Length: 345 *F Hi Tom, *F Thanks for the information regarding the Trf cluster of genes. *F The information has been curated as a personal communication from yourself *F to FlyBase. All genetic and molecular data will be captured and available *F in the public files within a few months. *F Thanks again for taking the time to provide the data, *F Eleanor Whitfield *F FlyBase *F From eleanor@gen.cam.ac.uk Tue Jul 15 08:56:30 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 08:56:30 +0100 *F To: tc45@columbia.edu *F Subject: Re: Trf cluster *F Cc: eleanor@gen.cam.ac.uk, ma11@gen.cam.ac.uk, *F ag24@gen.cam.ac.uk, gm119@gen.cam.ac.uk, *F rd120@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 15 Jul 1997 09:01:04 +0100 *F Content-Length: 525 *F Hi again, *F Apologies for the multiple replies, Michael and I were working on your *F message in different offices at the same time! *F Anyway .... *F Trf symbol is in FlyBase and will remain unchanged. *F Mcr is the same as alpha-2-Mcr and anon-28DE so these two shall be *F merged, but we cannot use Mcr as the gene symbol as it is already being *F used for myosin rod protein. Do you have an alternative symbol we can *F use? *F Trfp is the same as l(2)28DE so the gene will be renamed to Trfp. *F Thanks again, *F Eleanor Whitfield *F FlyBase *F From tc45@columbia.edu Tue Jul 15 21:24:13 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 21:24:13 +0100 *F Date: Tue, 15 Jul 1997 16:28:41 -0400 (EDT) *F X-Sender: tc45@pop.columbia.edu *F X-Mailer: Windows Eudora Light Version 1.5.4 (16) *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: Thomas Crowley *F Subject: Mcr *F Content-Length: 991 *F Dear Eleanor: *F Thanks for your response. Since the distal gene of the Trf cluster codes for *F a protein with sequence similarity to both macroglobulin and some of the *F complement proteins, it could be named: Mcr for Macroglobulin *F complement-related. Hopefully this designation isn't already being used, if *F it is let me know and we'll try again. *F Tom # *U FBrf0094744 *a Fristrom *b J.W. *t 1997.8.6 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Jul 15 16:03:34 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 16:03:34 +0100 *F To: fristrom@mendel.berkeley.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 15 Jul 1997 16:08:09 +0100 *F Content-Length: 1683 *F Dear Dr. Fristrom, *F I am curating your paper for FlyBase: *F Prout et al., 1997, Genetics 146(1): 275-285 *F I have the following queries about some of the genes mentioned in this paper. *F 1. penguin *F FlyBase already has a record for a gene called penguin (symbol pen) which *F is located in 19F on the X chromosome. *F The penguin gene mentioned in your paper is stated to be on 2L, and *F therefore can not be the same gene. I would be grateful if you could *F confirm that the location of your penguin gene is on 2L (to confirm that it *F is a different gene from the one already in FlyBase). Also I would be *F grateful if you could suggest a new name and symbol for this gene. *F 2. steamer duck *F The symbol you have used for steamer duck (stk) is already in use (for *F 'sticking'). I would be grateful if you could suggest another symbol for *F this gene. *F 3. cassowary *F The symbol you have used for cassowary (cas) is already in use (for *F 'castor'). I would be grateful if you could suggest another symbol for this *F gene. *F To check whether the new symbols you have decided on are already in use, I *F suggest you use 'Symbol Search' on FlyBase, which can be found under *F 'Searches' on the home page at the following site: *F http://flybase.bio.indiana.edu:80/ *F I look forward to hearing from you, *F Gillian Millburn *F \------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \------------------------------------------------------------- *F From fristrom@mendel.Berkeley.EDU Tue Jul 15 16:21:54 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 16:21:54 +0100 *F Date: Tue, 15 Jul 1997 08:26:29 -0700 (PDT) *F From: Jim Fristrom *F To: gm119@gen.cam.ac.uk *F Subject: Re: FlyBase query *F Content-Length: 288 *F Gillian: Thanks for your message. I suggest cass for cassowary and stck for steamer duck. As to penquin, it is my understanding that penquin on the X does not actually exist, so I need to research this further. *F Sorry for the trouble. Jim Fristrom *F From fristrom@mendel.berkeley.edu Wed Aug 06 21:43:24 1997 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 6 Aug 1997 21:43:24 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-mailer: Eudora Pro 3.0 for Macintosh *F Date: Wed, 6 Aug 1997 13:51:08 -0800 *F To: Gillian Millburn (Genetics) *F From: fristrom@mendel.berkeley.edu *F Subject: Re: FlyBase query *F Content-Length: 323 *F Gillian: Still doubt penguin exists. However, let's rename penguin as *F pygoscelis (pyg). *F Also, rhea is not at 75A-C but is elsewhere on 3L, *F Finally, the deficiency that fails to complement moa is Df(2R)59AB, *F not Df(2R),AB as it appears in table 1. *F Thanks for your help! *F Jim # *U FBrf0094745 *a Frolov *b M. *t 1997.8.1 *T personal communication to FlyBase *u *F >From mfrolov@biosci.mbp.missouri.edu Fri Aug 01 19:59:07 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 1 Aug 1997 19:59:07 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 1 Aug 1997 14:06:14 -0500 *F To: Michael Ashburner (Genetics) *F From: Maxim Frolov *F Subject: Re: Help FlyBase please *F Content-Length: 562 *F *F >I see I misunderstood - yes FlyBase already has Gap1 at 67D2.3. *F > *F >FlyBase knows about the following genes coding for GTPase-activating *F >proteins in addition to Gap1: (these mostly seem to be Ras activating) *F > *F >K230 at 13E *F >pn at 2E *F >Rapgap1 - not mapped *F >RasGap - not mapped *F >rn at 84D *F > *F > *F >I would suggest your gene's symbol to be Gap69C. Would that be OK ? *F > *F >Michael *F *F Thanks for the information about Drosophila GAPs. *F Gap69C is an excellent name. *F Both G3-81 and G3-85 are deletions within Gap69C. I included their *F breakpoints into GenBank record. *F Maxim. # *U FBrf0094820 *a Green *b M.M. *t 1997.8.18 *T personal communication to FlyBase *u *F Personal communication from: Mel Green *F To: FlyBase *F Date: August 18, 1997 *F Background: by phone *F Information communicated: r maps to the left of if, based on one crossover # *U FBrf0094967 *a Hochman *b B. *t 1997.7.29 *T personal communication to FlyBase *u *F From bhochman@utk.edu Mon Jul 28 18:40:44 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 28 Jul 1997 18:40:44 +0100 *F Date: Mon, 28 Jul 1997 13:45:40 -0400 (EDT) *F X-Sender: bhochman@popserver.utk.edu (Unverified) *F X-Mailer: Windows Eudora Light Version 1.5.2 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: rd120@gen.cam.ac.uk *F From: Benjamin Hochman *F Subject: FlyBase Inquiry *F Content-Length: 1895 *F Dear Rachel, *F I found an old stocklist ( from 1968 ) that may clear things up a bit. When *F we first got chromosome 4 lethals ( found by our lab or sent by others ) *F they were given designations and then tested for allelism ( inter se and with *F lethal stocks already present). Those found in a 1963 Knoxville orchard were *F labeled K-1 to K-7, and those sent by Alice Kenyon were labeled AK-39, etc. *F After allelism tests were finished, the lethal designations were changed. *F The table below gives the correct symbols and their former designations. *F CORRECT NOMENCLATURE FORMER DESIGNATION *F l(4)1e K-7 *F 1(4)2h K-5 *F l(4)16 K-4 *F l(4)20 K-3 *F l(4)29b K-1 *F l(4)1f AK-376 *F l(4)5a AK-354 *F l(4)10a AK-209 *F l(4)16b AK-39 *F l(4)16c AK-60 *F l(4)33c AK-82 *F Unfortunately, I could find no K-2 or K-6. But at least this gives some *F evidence as to the possible correspondence of K-1 and K-3 to stocks you *F already have. As to the stocks you call 'Kenyon' lethals, some corrections *F are in order. 16a was not found by Kenyon. Nor were 10 and 33. She found 10a *F and 33c, as well as 1f, 5a, 16b and 16c. *F You could run allelism tests of the lethal you presently call K-2 against *F the other chromosome 4 stocks in your collection. I assume all of these *F lethals are still balanced over ciD. If so, just collect a bunch of virgin *F females from the K-2 stock and cross them separately to males from the other *F stocks. If non-ciD progeny appear, the lethals are non-allelic. *F I hope this helps you and Kathy Matthews. *F Best wishes, *F Ben # *U FBrf0095296 *a Jacq *b B. *t 1997.7.1 *T personal communication to FlyBase *u *F >From jacq@ibdm.univ-mrs.fr Tue Jul 01 17:39:29 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 1 Jul 1997 17:39:29 +0100 *F Date: Tue, 1 Jul 1997 19:43:46 +0200 *F From: 'Bernard jacq' *F Reply-To: 'Bernard jacq' *F To: ma11@gen.cam.ac.uk *F Subject: Re: Help Flybase please *F Content-Length: 3995 *F *F In message Michael Ashburner (Genetics) *F writes: *F > *F > Bernard, *F > *F > I knew there was something else I wanted to ask you ! What is the *F > relationship *F > between Ariga's gene they call CP-1 encoding a CAAT-motif transcription *F > factor *F > (GenBank D43795) and corto ? I have an email from sometime ago *F > from Amos Bairoch who says they are the same, despite differences in *F > sequence. *F > *F > *F > Michael *F > *F > *F > ----- End Included Message ---- *F > *F --------------------------------------------------------------------------- *F Dear Mike, *F *F The answer is simple: it is the SAME gene, but we noted a lot of differences *F between the 2 sequences which cannot be attributed only to polymorphism. *F *F In the coding region for instance, we noted 51 differences, nearly all them *F causing a frameshift. When Ariga's sequence appeared in Genbank, we went back to *F all of our sequencing gels. I am quite confident from the detailed analysis we *F performed that our sequence is very close to the good one. In fact, we think at *F the moment that no change has to be introduced in our coding sequence. The thing *F is that there are a lot of regions where one finds 3 or more consecutive Cs or *F Gs, causing compressions. In the great majority of cases, Inosine was used to *F solve the ambiguities. Also, we verified the Drosophila codon usage of ccf *F (corto), which was satisfactory for nearly all of the coding region. *F *F Finally, as far as the function of ccf is concerned, I am very suspicious about *F the claimed CAAT-motif transcription factor function for this gene. I looked *F recently in Medline PubMed, and was not able to find a publication on this *F aspect. I just think they performed an in vitro binding (or transcription) test *F in heterologous conditions, but do not have any data on the Drosophila function. *F *F We have a paper on ccf in preparation, which will be sent back to EMBO J. in *F august, after some more experiments asked for by two referees and writing *F corrections will be made. *F If you need more details on ccf, just ask me. # *U FBrf0095782 *a Moses *b K. *t 1997.8.12 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Kevin Moses, University of Southern California *F To: Bloomington Drosophila Stock Center *F Subject: gl[BS1] *F Dated: 12 August 1997 *F *F Background: The Bloomington stock list incorrectly showed the gl allele on TM6B in the stock w[1118]; hh[ts2] e[s]/TM6B, gl[BS1] Tb[1] (#1684) as gl[BX1]. Moses reports that the correct allele is gl[BS1], which is not currently in FlyBase. *F *F Information communicated: *F *F gl[BS1] - glass Berkeley Spontaneous # *U FBrf0095787 *a Mukai *b M. *t 1997.7.16 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Jul 15 14:41:28 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 14:41:28 +0100 *F To: skob@sakura.cc.tsukuba.ac.jp *F Subject: Help FlyBase - \#098 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 15 Jul 1997 14:46:00 +0100 *F Content-Length: 550 *F Hi Satoru, *F I am curating an abstract of yours (A249) from the 16th International *F Congress of Developmental Biology (published in Dev. Biol. 186(2)) for *F FlyBase in which you discuss a new gene '#098'. *F As this is a new gene I was hoping you could provide a few more details *F than the abstract gave, namely: *F is this a temporary gene symbol and if so have you decided upon another *F symbol yet? *F do you know the location (genetic or cytological) of the gene? *F and any further details you would like to give me! *F Many thanks, *F Eleanor Whitfield *F FlyBase *F From mmukai@tara.tsukuba.ac.jp Wed Jul 16 04:22:00 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 16 Jul 1997 04:22:00 +0100 *F Date: Wed, 16 Jul 1997 12:31:34 +0900 *F To: eleanor@gen.cam.ac.uk *F From: mmukai@tara.tsukuba.ac.jp (Masanori Mukai) *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=iso-2022-jp *F X-Mailer: Eudora-J(1.3.8.5-J13) *F Content-Length: 405 *F Dear Dr. Eleanor Whitfield, *F I am a postdoc in Dr. Kobayashi's lab. '#098' is a temporary name. We *F have renamed the gene as 'indora'. The location of indora is 42A. Indora *F transcript was identified by a differential display screening. We now start *F genetics on the locus to reveal the function in germ line development. *F Sincerely yours, *F Masanori Mukai *F c/o Dr. Satoru Kobayashi University of Tsukuba *F From eleanor@gen.cam.ac.uk Wed Jul 16 08:12:21 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 16 Jul 1997 08:12:21 +0100 *F To: mmukai@tara.tsukuba.ac.jp *F Subject: Re: Help FlyBase - \#098 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 16 Jul 1997 08:16:48 +0100 *F Content-Length: 464 *F Hi Masanori, *F Thanks for your reply. *F One question: *F Do you have a short 3 or 4 letter symbol for the gene indora or will this *F represent the name and symbol? *F Thanks, *F Eleanor Whitfield *F From mmukai@tara.tsukuba.ac.jp Thu Jul 24 09:39:26 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Thu, 24 Jul 1997 09:39:26 +0100 *F Date: Thu, 24 Jul 1997 17:48:21 +0900 *F To: Eleanor Whitfield (Genetics) *F From: mmukai@tara.tsukuba.ac.jp (Masanori Mukai) *F Subject: Re: Help FlyBase - \#098 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=iso-2022-jp *F X-Mailer: Eudora-J(1.3.8.5-J13) *F Content-Length: 394 *F Dear Dr. Eleanor Whitfield *F 'indora' is the gene name and we have a symbol 'idr' for the gene 'indora'. *F 'indora' is japaneses god's name. *F Thanks, *F Masanori Mukai # *U FBrf0095959 *a O'Tousa *b J. *t 1997.8.18 *T personal communication to FlyBase *u *F >From O'Tousa.1@nd.edu Wed Aug 13 17:45:10 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 13 Aug 1997 17:45:10 +0100 *F X-Sender: Joseph.E.O'Tousa.1@nd.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 13 Aug 1997 11:49:08 -0500 *F To: Michael Ashburner (Genetics) *F From: 'O'Tousa.1'@nd.edu *F Subject: Re: Help FlyBase please *F Content-Length: 1254 *F Michael: Yes our rab6 is the same as Ozaki's. Now his published cytological *F position at 33C/D correct for rab6. We had given a incorrect position in *F our fly meeting abstract, but we subsequently confirmed his placement. I *F hope this helps. Joe. *F Joe O'Tousa, Dept. Bio. Sci., Univ. Notre Dame, Notre Dame, IN 46556 USA *F (219) 631-6093, fax 219-631-7413, email: 'o'tousa.1'@nd.edu (keep quotes in *F address if message won't go through). # *U FBrf0096092 *a Posakony *b J. *t 1997.8.7 *T personal communication to FlyBase *u *F From jposakony@ucsd.edu Tue Aug 05 20:10:23 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 5 Aug 1997 20:10:23 +0100 *F X-Sender: posakony@biomail.ucsd.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 5 Aug 1997 12:16:35 -0700 *F To: Rachel Drysdale (Genetics) *F From: Jim Posakony *F Subject: Re: Helping FlyBase *F Content-Length: 958 *F >Our Ref: rd2836.hf *F > *F >Hi Jim, *F > *F >I am curating *F > *F >*x FBrf0076568 == Hodgetts et al., 1994, Genome 37(4): 526-534. *F > *F >Figure 2 shows vector pARP-11, made by yourself. *F > *F >In order to capture the details of this paper I need to know a little more *F >about pARP-11, such as does the Adh bit correspond to any of these *F >Adh alleles? *F Now we're really going back into ancient history (the map I have is dated *F 10/82). The Adh bit in pARP-11 is actually none of these. It is an *F approximately 4.6-kb EcoRI/XhoI fragment that is just slightly shorter on *F its 3' end than the 4.8-kb EcoRI/EcoRI fragment carried in Adh+t4.8. It's *F the same AdhF allele, though. *F >and is the ry+ the well used ry+t7.2 (as used in Carnegie 20) or *F >another version? *F Naturally, I had to do things my own way. I preferred to use a somewhat *F larger ry+ piece, an 8.2-kb SalI fragment. *F Good luck with your curating, Rachel. Let me know if you have other questions. *F Best wishes, *F Jim *F From jposakony@ucsd.edu Wed Aug 06 23:27:07 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 6 Aug 1997 23:27:07 +0100 *F X-Sender: posakony@biomail.ucsd.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 6 Aug 1997 15:33:11 -0700 *F To: Rachel Drysdale (Genetics) *F From: Jim Posakony *F Subject: Re: Helping FlyBase *F Content-Length: 524 *F Hi Rachel, *F >I'm 99.9% sure that what you describe as an 8.2-kb SalI fragment must be *F >the Rubin and Spradling one represented in FlyBase by the allele: *F >*A ry+t8.1 *F >Could you confirm for me that this is the same one? *F Yes, you're right - no question about it. *F Best wishes, *F Jim # *U FBrf0096156 *a Riddiford *b L. *t 1997.7.7 *T personal communication to FlyBase *u *F From lmr@zookeeper.zoology.washington.edu Mon Jul 07 17:39:06 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 7 Jul 1997 17:39:06 +0100 *F Comments: Authenticated sender is *F From: 'Lynn M. Riddiford' *F To: Michael Ashburner (Genetics) *F Date: Mon, 7 Jul 1997 09:46:08 +0000 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=US-ASCII *F Content-transfer-encoding: 7BIT *F Subject: Re: cuticle gene nomenclature *F Reply-to: lmr@u.washington.edu *F CC: chihara@usfca.edu, flycam@gen.cam.ac.uk, *F flybase-harvard@morgan.harvard.edu, flybase-indiana@morgan.harvard.edu, *F flybase-ncbi@morgan.harvard.edu *F Priority: normal *F X-mailer: Pegasus Mail for Win32 (v2.54) *F Content-Length: 533 *F *F Dear Mike, *F Thanks for the help on the nomenclature. Everything is now *F clear and we will change them accordingly. The only difference is *F that it is dcp5 (not dcp3) that is the adult cuticle protein (guess *F that my 3's and 5's look similar). *F Therefore, for the map from left to right, it will be *F Acp65Aa (dcp5) *F Lcp65Aa (dcp4) *F Lcp65Ab1 (dcp3[beta])=Lcp5 *F Lcp65A&PSgr; (dcp4[pseudo]) *F Lcp65Ab2 (dcp3[alpha])=Lcp5 *F Lcp65Ac (dcp2) *F The remainder are as you had them. *F Lynn # *U FBrf0096184 *a Roote *b J. *t 1997.7.23 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Wed Jul 23 11:49:31 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Wed, 23 Jul 1997 11:49:31 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 23 Jul 1997 11:53:16 +0000 *F To: Aubrey de Grey *F From: John Roote *F Content-Length: 175 *F Hi Aubrey, *F Just to be sure that I maintain the record for the number of pers comms in *F FB here is another: *F Trudi Schupbach's fs(2)ltoRN48 is a cactus allele. *F Cheers, *F John # *U FBrf0096185 *a Roote *b J. *t 1997.8.13 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Wed Aug 13 08:40:07 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 13 Aug 1997 08:40:07 +0100 *F To: jr32@gen.cam.ac.uk *F Subject: l(2)CA61VS *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Wed, 13 Aug 1997 08:45:17 +0100 *F Content-Length: 477 *F Hi John, *F Kathy asked for l(2)CA61VS to be entered into FlyBase. *F It is a new gene-allele for us and was apparently provided by you in this *F stock: *F b1 esg35Ce-1 pr1 cn1 bw1 l(2)CA61VS/SM5 *F quote from Kathy: *F 'which was placed in the Mid-America Stock Center by the Ashburner lab *F and is now in the Bloomington collection.' *F Is there anything you can tell us about this stock: *F mutagen *F phenotype (I know it is lethal but at what stage etc) *F discoverer *F etc *F thanks, *F Ele *F From jr32@mole.bio.cam.ac.uk Wed Aug 13 11:46:57 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 13 Aug 1997 11:46:57 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 13 Aug 1997 11:51:21 +0000 *F To: Eleanor Whitfield (Genetics) *F From: John Roote *F Subject: Re: l(2)CA61VS *F Content-Length: 914 *F Hi Ele, *F CA61 is an unmapped gene whose existence is inferred from the lack of *F complementation between VS2, VS8 (both esg alleles) and Df(2L)b80k, *F Df(2L)b88c25 (neither of which remove esg), i.e. all four stocks are *F mutant for CA61. *F Df(2L)b80k and Df(2L)b88c25 complement all other esg alleles. *F VS2 and VS8 are from the same screen of Pat Simpson. *F b80k and b88c25 are from Alexandrov (but 8 years apart). *F John *F From eleanor@gen.cam.ac.uk Fri Aug 15 08:20:04 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 15 Aug 1997 08:20:04 +0100 *F To: jr32@gen.cam.ac.uk *F Subject: l(2)35CcAM7 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 15 Aug 1997 08:25:16 +0100 *F Content-Length: 241 *F Kathy wrote: *F >I have l(2)35CcAM7 in a stock from Bowling Green, to them from the *F >Ashburner lab. Perhaps you could get a pc on this one too from JR32. *F How about it then John??!! *F We have the gene but know nothing about the mutation. *F Ele *F From jr32@mole.bio.cam.ac.uk Fri Aug 15 10:09:08 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 15 Aug 1997 10:09:08 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 15 Aug 1997 10:13:45 +0000 *F To: Eleanor Whitfield (Genetics) *F From: John Roote *F Subject: Re: l(2)35CcAM7 *F Content-Length: 423 *F The stock is rd9. *F The existence of 35Cc is inferred from the lethality of rd9. I guess rd9 *F cannot be both a deletion and a point mutation of 35Cc. *F Take your pick! *F John # *U FBrf0096186 *a Roote *b J. *t 1997.8.20 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Mon Aug 18 12:20:38 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Mon, 18 Aug 1997 12:20:38 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Mon, 18 Aug 1997 12:25:21 +0000 *F To: Aubrey de Grey *F From: John Roote *F Subject: Re: cyto of T(2;3)TE35B-3 *F Content-Length: 663 *F >Hi John, *F > *F >In DIS 77 you have T(2;3)TE35B-3 broken in '86F12-87A2'. Sorsa (at *F >least) denies the existence of an 86F12, though 86F11 exists. I am *F >thus torn between the equally common problems that (a) the cyto was *F >done wrt a map that had an 86F12 or (b) it was originally '86F1,2'. *F >Do you happen to have any notes that can illuminate this? *F > *F >Cheers, Aubrey *F Well its funny you should ask that. I've just discovered that MA's entry *F for GR3 into the original Adh cyt list had a mistake. It should have been *F 35B;85F12-86A2 - for 2 reasons: *F 1) another entry says 35B;85A1.2 *F 2) the segregants GR3 G16 and G16 GR3 are both healthy stocks. *F Sorry again, *F John # *U FBrf0096281 *a Settleman *b J. *c I. *d Hariharan *t 1997.8.8 *T personal communication to FlyBase *u *F From settleman@helix.mgh.harvard.edu Fri Aug 08 18:33:36 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 8 Aug 1997 18:33:36 +0100 *F Date: Fri, 08 Aug 1997 13:39:38 -0500 *F From: settleman@helix.mgh.harvard.edu (Jeffrey Settleman) *F Subject: map correction *F X-Sender: settlema@helix.mgh.harvard.edu *F To: flybase-updates@morgan.harvard.edu *F Content-transfer-encoding: 7BIT *F Content-Length: 256 *F We would like to correct the cytological map position of the Rho1 gene, *F which we previously reported at 3C1-3C12. The revised assignment is to map *F location 52E3-6, as reported by Strutt et al. Nature 387:292-294 (1997). *F Jeffrey Settleman *F Iswar Hariharan # *U FBrf0096282 *a Settleman *b J. *c K. *d Barrett *t 1997.8.8 *T personal communication to FlyBase *u *F From settleman@helix.mgh.harvard.edu Fri Aug 08 18:42:27 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 8 Aug 1997 18:42:27 +0100 *F Date: Fri, 08 Aug 1997 13:48:28 -0500 *F From: settleman@helix.mgh.harvard.edu (Jeffrey Settleman) *F Subject: submission to Flybase *F X-Sender: settlema@helix.mgh.harvard.edu *F To: flybase-updates@morgan.harvard.edu *F Content-transfer-encoding: 7BIT *F Content-Length: 397 *F We would like to submit a novel gene identification to Flybase. The gene *F has been named shar pei, and encodes a guanine nucleotide exchange factor *F for the Rho1 GTPase. The gene has been mapped cytologically to 53F. This *F gene was reported at the 1997 annual Drosophila Research Conference in *F abstract 12C. Please reply if more information is required. *F Sincerely, *F Jeffrey Settleman *F Kathy Barrett *F From rd120@gen.cam.ac.uk Mon Aug 11 11:12:19 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 11 Aug 1997 11:12:19 +0100 *F To: settleman@helix.mgh.harvard.edu *F Subject: Re: submission to Flybase *F Cc: rd120@gen.cam.ac.uk *F From: Rachel Drysdale (Genetics) *F Date: Mon, 11 Aug 1997 11:17:32 +0100 *F Content-Length: 658 *F Our Ref: rd2853 *F Hi Jeffrey and Kathy, *F we like to keep a note of the meaning of gene symbols in FlyBase, where we *F can. Could you let me know what 'shar pei' means, and I'll make sure an *F explanation is included in our files. *F Many thanks, *F Rachel. *F From settleman@helix.mgh.harvard.edu Mon Aug 11 13:05:01 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 11 Aug 1997 13:05:01 +0100 *F Date: Mon, 11 Aug 1997 08:11:00 -0500 *F To: Rachel Drysdale (Genetics) *F From: settleman@helix.mgh.harvard.edu (Jeffrey Settleman) *F X-Sender: settlema@helix.mgh.harvard.edu *F Subject: Re: submission to Flybase *F Content-Length: 268 *F Rachel, *F Thanks for handling our gene submission. The name 'shar pei' is the *F name of a breed of dog characterized by numerous skin folds, and reflects *F the inappropriate lateral folds of embryos lacking shar pei (generated as *F germline clones). *F Jeff Settleman *F From settleman@helix.mgh.harvard.edu Mon Aug 11 13:29:48 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 11 Aug 1997 13:29:48 +0100 *F Date: Mon, 11 Aug 1997 08:34:57 -0500 *F To: Rachel Drysdale (Genetics) *F From: settleman@helix.mgh.harvard.edu (Jeffrey Settleman) *F X-Sender: settlema@helix.mgh.harvard.edu *F Subject: Re: submission to Flybase *F Content-Length: 132 *F Rachel, *F We've been abbreviating it 'shar' since most of the obvious *F 3-letter names are being used already. *F Thanks, *F Jeff # *U FBrf0097863 *a Wolff *b T. *t 1997.7.16 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Jul 15 14:34:01 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 15 Jul 1997 14:34:01 +0100 *F To: twolff@uclink4.berkeley.edu *F Subject: Help FlyBase - strabismus *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 15 Jul 1997 14:38:32 +0100 *F Content-Length: 1357 *F Hi Tanya, *F I am curating an abstract of yours (S34) from the 16th International *F Congress of Developmental Biology (published in Dev. Biol. 186(2)) for *F FlyBase in which you discuss a new gene 'strabismus'. Unfortunately you *F use the short symbol 'stb' for this gene and this has already been used for *F 'short bristle' by Fahmy in 1958! *F Gerry is here at the moment (Uni. Camb) and he has suggested 'stab' as an *F alternative symbol. *F Are you happy with this? *F As this is a new gene to FlyBase would you also be prepared to provide a *F few more details that the abstract gave, namely: *F the location (genetic or cytological) of strabismus *F what does strabismus mean? *F allele designation for the mutant described in the abstract *F and anything else you would be prepared to declare! *F Many thanks for your help, *F Eleanor Whitfield *F FlyBase *F From twolff@uclink4.berkeley.edu Wed Jul 16 14:50:33 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 16 Jul 1997 14:50:33 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 16 Jul 1997 06:55:32 -0700 *F To: eleanor@gen.cam.ac.uk *F From: twolff@uclink4.berkeley.edu (Tanya Wolff) *F Subject: strabismus *F Content-Length: 901 *F Hi Eleanor, *F Thank you for your email message. I was aware that stb had already been *F used as an abbreviation for a mutant which, according to the red book, no *F longer exists. I wasn't sure whether it was legitimate to use this *F abbreviation or not, given there is no longer a mutant to go with the name, *F and was going to check with Gerry before submitting a paper and making it *F official, so I guess now I don't need to ask him! I would prefer to go with *F 'strb' as opposed to 'stab'. strb has been genetically mapped to 2-61 and *F cytologically mapped to 45A-B. The allele described in the abstract is strb *F 6, and the definition of strabismus is : a disorder in vision due to a *F deviation from normal orientation of one or both eyes; it is the medical *F term for cross-eyed. If you have any further questions, please feel free *F to ask. *F Sincerely, *F Tanya Wolff # *U FBrf0097956 *a Yamamoto *b D. *t 1997.8.25 *T personal communication to FlyBase *u *F Dear Dr. Yamamoto, *F I am writing about your lingerer line which you wrote about in *F \*x FBrf0092235 == Kuniyoshi and Yamamoto, 1997, A. Conf. Dros. Res. 38: 220B *F I have come across it as part of an editing job that I am currently *F engaged in, and I wonder whether you have a symbol for the insertion *F allele yet, also which P element has inserted in the allele? *F Many thanks for your help, *F Rachel. *F \--------------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \--------------------------------------------------------------------- *F From daichan@fly.erato.jst.go.jp Mon Aug 25 10:50:46 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 25 Aug 1997 10:50:46 +0100 *F Date: Mon, 25 Aug 1997 19:00:38 +0900 *F To: rd120@gen.cam.ac.uk *F From: daichan@fly.erato.jst.go.jp (YAMAMOTO, Daisuke) *F X-Sender: daichan@202.241.18.161 *F Subject: lingerer *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=iso-2022-jp *F X-Mailer: Eudora-JE(1.3.8-JE13) *F Content-Length: 771 *F Dear Rachel, *F We call the P-element induced allele lingerer. The *F abbreviated symbol is lig. The P-element inserted is Bm delta-w. A *F brief description about the lig mutation has been given in my review *F article appeared in Annu. Rev. Entomol. 42:551-585. Hope this information *F is useful for you. *F Best wishes, *F Daisuke *F \------------------------------------------------------ *F YAMAMOTO, Daisuke *F ERATO, Mitsubishi-Kasei Institute of Life Sciences, *F Minami-Ooya 11, 194 Machida-shi, Tokyo, Japan *F Tel: (81) 427-21-2334 *F Fax: (81) 427-21-2850 *F email : daichan@fly.erato.jst.go.jp *F \------------------------------------------------------ # *U FBrf0098056 *a Frasch *b M. *t 1997.7.23 *T personal communication to FlyBase *u *F From nobody Wed Jul 23 22:34:41 1997 *F Date: Wed, 23 Jul 97 22:34:40 EDT *F From: nobody *F To: crosby@morgan.harvard.edu, flybase-archive@morgan.harvard.edu, *F frasch@msvax.mssm.edu *F Subject: GAL4/UAS Submission to FlyBase - FBgu0000108 *F Content-Length: 1051 *F *F GAL4/UAS Submission Number: FBgu0000108 (Wed Jul 23 22:34:39 1997) *F *F lastname : *F Frasch *F givenname : *F Manfred *F institution : *F Mount Sinai School of Medicine *F email : *F frasch@msvax.mssm.edu *F author1 : *F Azpiazu *F author2 : *F et al. *F year : *F 1996 *F journal : *F Genes Dev. *F volume : *F 10 *F start_page : *F 3183 *F end_page : *F 3194 *F transposon : *F P{UAShh.1} *F vector : *F pP{UAST} *F cDNA : *F hedgehog *F insert_size : *F 2.3kb *F UAS_comments : *F contains full length hh cDNA #11 (from P. Beachy) *F insertion_symbol_1 : *F P{UAShh.1}III *F chromosome_1 : *F 3 *F insertion_property_1 : *F lethal *F *F +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ *F From nobody Wed Jul 23 22:41:57 1997 *F Date: Wed, 23 Jul 97 22:41:57 EDT *F From: nobody *F To: crosby@morgan.harvard.edu, flybase-archive@morgan.harvard.edu, *F frasch@msvax.mssm.edu *F Subject: GAL4/UAS Submission to FlyBase - FBgu0000109 *F Content-Length: 1018 *F *F GAL4/UAS Submission Number: FBgu0000109 (Wed Jul 23 22:41:55 1997) *F *F lastname : *F Frasch *F givenname : *F Manfred *F institution : *F Mount Sinai School of Medicine *F email : *F frasch@msvax.mssm.edu *F author1 : *F Azpiazu *F author2 : *F et al. *F year : *F 1996 *F journal : *F Genes Dev. *F volume : *F 10 *F start_page : *F 3183 *F end_page : *F 3194 *F transposon : *F P{UAS-wg.2} *F vector : *F pP{UAST} *F cDNA : *F wg/wingless *F UAS_comments : *F contains wg cDNA c14 (from N. Baker) *F insertion_symbol_1 : *F P{UAS-wg.2}III *F chromosome_1 : *F 3 *F insertion_property_1 : *F lethal *F *F +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ *F From nobody Wed Jul 23 22:48:54 1997 *F Date: Wed, 23 Jul 97 22:48:53 EDT *F From: nobody *F To: crosby@morgan.harvard.edu, flybase-archive@morgan.harvard.edu, *F frasch@msvax.mssm.edu *F Subject: GAL4/UAS Submission to FlyBase - FBgu0000110 *F Content-Length: 1026 *F *F GAL4/UAS Submission Number: FBgu0000110 (Wed Jul 23 22:48:53 1997) *F *F lastname : *F Frasch *F givenname : *F Manfred *F institution : *F Mount Sinai School of Medicine *F email : *F frasch@msvax.mssm.edu *F author1 : *F Frasch *F year : *F 1995 *F journal : *F Nature *F volume : *F 374 *F start_page : *F 464 *F end_page : *F 467 *F transposon : *F P{UAS-dpp.BEH1} *F vector : *F pP{UAST} *F cDNA : *F dpp *F UAS_comments : *F contains dpp cDNA BEH1 (St Johnston et al, 1990) *F insertion_symbol_1 : *F P{UAS-dpp.BEH1}5 *F chromosome_1 : *F 3 *F insertion_property_1 : *F semi-lethal ; visible_phenotype *F *F +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ *F # *U FBrf0098074 *a Ozaki *b K. *t 1997.8.1 *T personal communication to FlyBase *u *F From ozaki@chaos.bio.sci.osaka-u.ac.jp Fri Aug 01 22:34:27 1997 *F Envelope-to: m.ashburner@gen.cam.ac.uk *F Delivery-date: Fri, 1 Aug 1997 22:34:27 +0100 *F Date: Fri, 1 Aug 1997 20:30:15 +0900 *F To: m.ashburner@gen.cam.ac.uk *F From: ozaki@chaos.bio.sci.osaka-u.ac.jp (Koichi Ozaki) *F X-Sender: ozaki@chaos.bio.sci.osaka-u.ac.jp *F Subject: Re: Help FlyBase please *F Mime-Version: 1.0 *F Content-Type: text/plain; charset=iso-2022-jp *F X-Mailer: Eudora-J(1.3.8.6-J13) *F Content-Length: 3879 *F *F Dear Professor Ashburner: *F *F Thank you very much for your e-mail, and I would apologize to you for *F making your head confused. *F *F In our recent paper (1997 FEBS Letters 404:65--69), we described the *F complete cDNA sequence of Drosophila Rab proteins, *F *F DRab 1,2,6,8,10,11,14, *F DRabRP3, RP4, *F *F which we registered at DDBJ DNA databank (linking EMBL and Genebank) *F in April,1996 (except DRab10, we registered the sequence today to open *F immediately). I asked them to release these sequences, when our paper *F in FEBS Lett. was published. But they did not. Today, I again asked DDBJ *F to open these sequence information, and I hope they will be released *F in a few days. *F *F Before the paper, we presented partial sequences of those DRabs at the *F meetings of Zoological Society of Japan (ZSJ) in 1993 and 1994. We also *F presented the complete cDNA sequence of them in the 1995 meeting of ZSJ. *F The following is the abstract of one of these meetings, where 'Kohno' *F is the old name of 'Satoh', who changed her family name with marriage. *F *F >An abstract by Kohno et al (1994 Zool. Sci., Tokyo Suppl. 11: 99.) *F >also mentions: *F > *F >DRAB1,2,3,4,7,8,10,11,14 *F >& *F >DRABR1,2,3,4 *F *F Other abstracts are as follows: *F *F Kono, A., Tokunaga, F. and Ozaki, K., (1993). Molecular cloning of *F rab-family proteins from Drosophila retina. Zool. Sci., 10, supple., 119. *F *F Kono, A., Ozaki, K., Tokunaga, F. and Tanimura, T., (1995). Drosophila *F Rab proteins; Determination of nucleotide sequences and gene loci, and *F the production of their dominant negative mutants. Zool. Sci., 12, *F supple., 113. *F *F *F In the above, DRab3, 4, 7, and DRabRP1, RP2 were not completely sequenced, *F when the paper in FEBS Lett. was published, and we did not register *F them in a DNA databank. However, partial sequence of DRab3 is completely *F identical to that has been determined by Johnston et al.(1991, Neuron 7: *F 101--109). *F *F The DRab genes from Hotta's Lab (DRab2 and DRab11) are the same as ours, *F which were, however, independently sequenced. *F *F >Shetty et al., 1996, A. Conf. Dros. Res. 37: 360 *F >Shetty et al., 1997, A. Conf. Dros. Res. 38: 216A *F > *F >describe a rab6 *F *F I am sorry I do not know these reports, but I assume these may be from *F Joe O'Tousa's group. If so, above Rab6 must be identical to ours, because *F we checked our sequences each other, last December. *F *F *F >[Please note that, following Lindsley & Zill the Drosophila gene naming *F >rules make it illegal to have D (=Drosophila) as a gene symbol prefix. All *F >Drosophila genes would have this otherwise ! *F > *F >I wonder if the time has not come to have a rational naming, after the *F >cytological position. *F >eg Rab2 would become Rab42C *F >Rab6 (of Satoh) Rab33B *F >etc. *F *F On the naming of Drosophila Rab gene, I do not think it good way to use *F cytological position. In Rab protein, the number following to the word *F 'rab' has a significant meaning. For example, rab1 forms very conservative *F subfamily consisting of the members from yeast rab1 to human rab1. These *F membaers have significantly different amino acid sequences from other *F subfamilies of Rab (e.g. Rab2, Rab3 ........). Therefore, Rab protein *F named,for example, Rab42C, generally means a protein belonging to a *F Rab42 subfamily. This would cause undesirable confusion. I rather propose *F the usage of the name 'rab1', 'rab2', 'rab3' ....... (Of course, I know *F 'rab' is already used for 'rabbit', but 'rab1' et al. is usable, isn't it?) *F *F Thank you again for your kind e-mail. I would like to pay my sincere *F respect to you for your great effort to flybase. *F *F Sincerely yours, *F *F Koichi *F *F *F ************************************************** *F Koichi Ozaki, D.Sc. *F *F Department of Biology, Graduate School of Science, *F Osaka University *F Toyonaka, Osaka 560, Japan *F Ph & FAX : +81-6-850-5439 *F e-mail : ozaki@bio.sci.osaka-u.ac.jp *F WWW : http://www.bio.sci.osaka-u.ac.jp/~ozaki/ *F ************************************************** # *U FBrf0098091 *a Yang *b M. *t 1996.9.13 *T personal communication to FlyBase *u *F >From gbga61@udcf.gla.ac.uk Fri Sep 13 11:39:56 1996 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Fri, 13 Sep 1996 11:38:43 -0500 *F To: ma11@gen.cam.ac.uk *F From: gbga61@udcf.gla.ac.uk (Mingyao Yang) *F Subject: Re: Help FlyBase *F Content-Length: 1032 *F *F >From: Michael Ashburner (Genetics) *F >Date: Thu, 12 Sep 96 17:52:37 BST *F >To: k.kaiser *F >Subject: Help FlyBase *F >Cc: ma11@gen.cam.ac.uk *F > *F >You recently deposited the sequence X98402 is EMBL described *F >as an alkaline phosphatase mapping to 100B4-5. *F >FlyBase knows about: *F > *F >*a Aph-1 *F >*b 3-47.3 (between W and p) (Wallis and Fox). *F >& *F >*a Aph-2 *F >*b 2- not mapped. *F >& *F >*a Aph-3 *F >*b not mapped. *F > *F >Is it one of these ? I presume not given its map location. *F > *F >Michael *F *F No. We don't think our alkaline phosphatase is one of the above. We presume *F it is a new one. We may name as 'Aph-4' if you agree. *F Kim & Yang *F *F From: Dr. Mingyao Yang *F Division of Molecular Genetics *F University of Glasgow *F Church street, G11 5JS *F Tel: 0141 330 6234 *F Fax: 0141 330 5994 *F E-mail: gbga61@udcf.gla.ac.uk # *U FBrf0098092 *a Monastirioti *b M. *t 1996.5.15 *T personal communication to FlyBase *u *F >From monastirioti@nefeli.imbb.forth.gr Wed May 15 10:47:53 1996 *F Date: Wed, 15 May 96 10:47:41 BST *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Michael Ashburner (Genetics) (by way of *F inga@nefeli.imbb.forth.gr (Inga Siden-Kiamos)) *F From: monastirioti@nefeli.imbb.forth.gr *F Subject: Re: For Maria Monastirioti *F Content-Length: 1430 *F *F Dear Michael *F *F My EMBL sequence record Z70316 refers to a cDNA for Tyramine *F beta-hydroxylase gene- disruption of this gene results in blockage of *F octopamine biosynthesis. The deduced aa sequence of the encoded protein *F excibits homology to the evolutionarily related mamalian Dopamine *F beta-hdroxylase (in fact we have cloned Tbh cDNA using aa sequence *F information from mamalian DBH, as the annotation in the EMBL record says). *F It might sounds confusing but one could look in the reference: *F Monastirioti, Linn and White 1996 J.Neuroscience in press, for more *F information and/or justification on the function of the gene. *F *F As of the information already existing in the Flybase: I see that it relies *F on titles of abstracts I have sent to meetings during the past years and *F represent different stages of my work when my data were not such to clarify *F the function of the gene that I had cloned. *F Finally, at this point, I do not know whether the cloned Tbh gene or *F another gene, yet unknown, has Dopamine beta hydroxylase function. *F I hope I helped in 'resolving' the confusion. If there are more or *F unanswered questions, please feel free to contact me again. I will be glad *F to help. *F *F Regards *F Maria # *U FBrf0098093 *a Gelbart *b W.M. *t 1997.5.8 *T personal communication to FlyBase *u *F >From gelbart@morgan.harvard.edu Thu May 08 14:51:03 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 8 May 1997 14:51:03 +0100 *F Date: Thu, 08 May 1997 09:47:36 -0400 *F From: gelbart@morgan.harvard.edu (William Gelbart) *F Subject: X97775: bgcn -> Tgfbeta-60A *F To: flybase-updates@morgan.harvard.edu *F Cc: gelbart@morgan.harvard.edu *F Content-transfer-encoding: 7BIT *F Content-Length: 338 *F *F Michael, *F *F The accession X97775 contains the coding sequences of *F Tgfbeta-60A, not bgcn. There is no obvious NA sequence *F level alignment between this accession and the other one *F ascribed to bgcn (X97641). This latter one may indeed *F be bgcn, although there is nothing in the database entry *F to make one confident about this. *F *F Bill # *U FBrf0098116 *a Hotta *b Y. *t 1997.8.27 *T personal communication to FlyBase *u *F From yhotta@phys.s.u-tokyo.ac.jp Wed Aug 27 10:11:04 1997 *F Subject: Drosophila Rab *F *F E-mail from Yoshiki Hotta at Univ. of Tokyo. *F *F Dear Mike, *F *F Thank you very much for your fax asking about our small-G clones. *F I am sorry that my reply is so belated. I have been visiting Kyusyu *F Univ. to attend Japan Drosophila Meeting. *F *F The following is my reply to your mail based on the discussion with *F my graduate student, T. Sasamura who is the major investigator of our *F small-G project. I also discussed the matter with Dr. Ozeki to confirm *F that our replies do not contradict each other to confuse you. *F *F You mentioned about 8 genes as our cloned rab genes. However, only *F 3 are members belonging to rab family (Drab2, Drab5, and Drab11), and *F others are of rho family, whose function must be different from rab's. *F So the other 5 genes have nothing to do with the classification of *F Drosophila rab genes. *F *F Among our rab genes, Drab2 and Drab11 must be identical to Mr.Ozaki's *F Drab2 and Drab11, since their cytological positions are identical. *F We consider that our Drab5 is a new gene that has been not cloned by *F other groups. The sequences of these genes were recently deposited to *F GenBank. *F *F You proposed that the name of Drosophila rab gene should be changed *F by its cytogenetic position(e.g. Rab42C or Rab33B), however we do not *F think it is appropriate. The names of rab genes we as well as Ozaki's *F group used are derived from its mamalian counterpart (e.g. Drab2 is *F a homologue of mammalian rab2). There are so many mammalian rab genes, *F whose functions must be classified by their homology. If the names are *F changed as you suggested, the correspondence between Drosophila's and *F mammalian genes are totally lost. I understand that gene names should *F be neutral, but still we think that the immediate understanding of the *F correspondence is very convenient and necessary at present. *F *F *F With best wishes, *F *F Yoshiki Hotta *F ----------------------------------------------------- *F Yoshiki Hotta *F Dept. of Physics & Mol. Genetics Research Lab., *F Graduate School of Science, The University of Tokyo *F Hongo, Bunkyo-ku, Tokyo 113 JAPAN *F Tel: +81-3-3812-2111 ext.4144, Fax: +81-3-3814-9717 *F E-mail: yhotta@phys.s.u-tokyo.ac.jp *F ----------------------------------------------------- # *U FBrf0098117 *a Coyne *b J. *t 1997.1.2 *T personal communication to FlyBase *u *F Archived. # *U FBrf0098118 *a Levin *b L.R. *t 1997.9.28 *T personal communication to FlyBase *u *F From llevin@mail.med.cornell.edu Sun Sep 28 19:29:31 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Sun, 28 Sep 1997 19:29:31 +0100 *F Mime-Version: 1.0 *F Content-Type: text/enriched; charset='us-ascii' *F Date: Sun, 28 Sep 1997 14:36:36 -0500 *F To: Michael Ashburner (Genetics) *F From: 'Lonny R. Levin' *F Subject: Re: Help FlyBase please *F Content-Length: 2164 *F *F >Dear Lonny *F > *F >I am curating your sequence record AF005630 for FlyBase. *F >I note this maps to 35C7-9. FlyBase already has a gene called Ac34A *F >with these references: *F > *F >*x FBrf0078595 == Han and Davis, 1995, A. Conf. Dros. Res. 36: 91B *F >*x FBrf0055565 == Levin et al., 1992, Cell 68: 479--489 *F > *F >Are these the same ? And is 35C7-9 the correct map position ? *F > *F >Thanks *F > *F >Michael Ashburner. *F *F Dear Michael, *F *F These refer to distinct Adenylyl cyclase isozymes. *F *F The recently published DAC9 hybridized to a P1 clone which had been *F mapped to 35C7-9 by BDGP. My laboratory never attempted to confirm this *F map position independently. *F *F AC-34A was published (in the papers you refer to) as a genomic fragment *F with homology to mammalian ACs. Its map position was determined by *F in situ hybridization. We have recently confirmed that *F there is indeed an AC isoform encoded by this genomic locus and it is *F distinct from DAC9, but have not published its sequence or submitted it *F to Genbank yet. *F *F Thank you for your work in maintaining this database. *F *F Lonny R. Levin *F *F Department of Pharmacology *F Cornell University Medical College *F 1300 York Avenue Rm. E-505 *F New York, NY 10021 *F Office: 212-746-6752 *F Lab: 212-746-6750 *F FAX: 212-746-8835 *F llevin@mail.med.cornell.edu *F http://www-users.med.cornell.edu/~llevin/default.html *F http://www-users.med.cornell.edu/~llevin/GPIP/PharmProgram.html # *U FBrf0098588 *a Sun *b H. *t 1997.8.30 *T personal communication to FlyBase *u *F From mbyhsun@ccvax.sinica.edu.tw Sat Aug 30 02:46:59 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Sat, 30 Aug 1997 02:46:59 +0100 *F Date: Sat, 30 Aug 1997 09:53:18 +0800 *F From: Henry *F Subject: Re: mirr/Sai/crep nomenclature. *F To: 'Rachel Drysdale (Genetics)' *F Cc: kchoi@bcm.tmc.edu, chyang@leland.Stanford.EDU, atc12@gen.cam.ac.uk, *F dario.coen@emex.u-psud.fr, ma11@gen.cam.ac.uk *F MIME-version: 1.0 *F X-Mailer: Mozilla 3.01 (Macintosh; I; 68K) *F Content-Type: text/plain; charset=us-ascii *F Content-transfer-encoding: 7bit *F Content-Length: 998 *F Dear Rachel: *F The lethality of DH-1 and DH-2 (FBrf0084424) were not complemented by Sai1 and Sai2. *F Best wishes. *F Henry # *U FBrf0098589 *a Coen *b D. *t 1997.8.28 *T personal communication to FlyBase *u *F From dario.coen@emex.u-psud.fr Thu Aug 28 14:08:18 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 28 Aug 1997 14:08:18 +0100 *F X-Sender: coen@psisun.u-psud.fr *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 28 Aug 1997 15:20:50 +0200 *F To: Rachel Drysdale (Genetics) *F From: Dario Coen *F Subject: Re: mirr/Sai/crep nomenclature. *F Content-Length: 390 *F Dear Rachel, *F I have found that the lethality of DH and DH2 is allelic to the *F lethality of crep3. *F Best *F Dario *F Dario COEN *F Embryologie Moleculaire et Experimentale *F Universite Paris Sud - Batiment 445 *F F-91405 Orsay cedex FRANCE *F tel: 33 1 69 15 68 88 *F fax: 33 1 69 15 68 02 *F e-mail: dario.coen@emex.u-psud.fr # *U FBrf0098590 *a Choi *b K. *t 1997.8.28 *T personal communication to FlyBase *u *F From kchoi@bcm.tmc.edu Thu Aug 28 17:14:22 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 28 Aug 1997 17:14:22 +0100 *F X-Sender: kchoi@postoffice.bcm.tmc.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 28 Aug 1997 11:22:05 -0600 *F To: rd120@gen.cam.ac.uk *F From: kchoi@bcm.tmc.edu (kwang choi) *F Subject: crep/mirr/sai nomenclature *F Content-Length: 747 *F Dear Rachel, *F Thanks for your notes. I also have a thing just for the record: Choi et al *F (PNAS 93, 5737-5741, 1996) have described an enhancer trap strain B1-12, *F which turned out to be allelic to sai/mirr. *F We renamed B1-12 as sailB1-12 in a submitted manuscript, since we noticed *F that Adelaide's alleles were the first mutations, were described in *F FlyBase, and the name describes the recessive phenotype. Should we rename *F it to mirr? *F With regards, *F Kwang Choi *F \******************************************** *F Kwang-W. Choi *F Dept. of Cell Biology, M820 *F Baylor College of Medicine *F One Baylor Plaza *F Houston, TX 77030 *F Office: 713-798-8649 *F Lab: 713-798-8554 *F Fax: 713-798-8005 *F e-mail: kchoi@bcm.tmc.edu *F \******************************************** # *U FBrf0098592 *a Rodriguez *b A. *t 1997.9.8 *T personal communication to FlyBase *u *F From rodrig02@UTSW.SWMED.EDU Mon Sep 08 16:56:50 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 8 Sep 1997 16:56:50 +0100 *F Date: Mon, 08 Sep 1997 11:05:31 -0500 *F From: rodrig02@UTSW.SWMED.EDU (Antony Rodriquez) *F Subject: Dredd gene correction *F To: flybase-updates@morgan.harvard.edu *F MIME-version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-transfer-encoding: 7BIT *F Content-Length: 771 *F To Whom Concerned, *F We would like to correct a mistake in flybase. In the 1B13 cytological *F region, there is a listing for a Redd gene, instead the name and symbol for *F this gene should read Dredd (GenBank G2199582). Also the Dredd gene is the *F same as the anon-1BCa (our unpublished observation) gene referenced in *F Voelker et al., 1989, Genetics 122: 625-642. Additionally there is a *F mistake in the molecular map data of that region (Steinhauer, W., and *F Voelker, R. personal communication). The actual physical map should read as *F follows: M(1)1B (1kb transcript), l(1)1Bi (3.5kb transcript), Dredd, su(s), *F anon-1BCb, and l(1)1Bk. Thanks in advance. *F Antony Rodriguez *F UT Southwestern Med. Ctr *F Dept. of Cell Biology & Neuroscience *F 5323 Harry Hines Blvd. *F Dallas, TX 75235 # *U FBrf0098593 *a Carpenter *b A.T.C. *t 1997.9.12 *T personal communication to FlyBase *u *F >From atc12@mole.bio.cam.ac.uk Thu Sep 11 22:49:48 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Thu, 11 Sep 1997 22:49:48 +0100 *F Date: Thu, 11 Sep 1997 22:55:40 +0100 (BST) *F From: Adelaide T C Carpenter *F To: Aubrey de Grey *F Subject: Official to FlyBase *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 1102 *F The mirr/cre/DH/B12/Sai/sai alleles on the D1 and D3 chromosomes are *F different as judged by viability of heterozygotes with Henry Sun's DH-1 *F allele (which is itself hypomorphic for the viability effect *F since it is homozygous viable, though with recessive outheld wings and *F reduced alulae, once the extraneous lethal(s) had been crossed off). D1 *F on CxD/DH is completely lethal; D1 on DcxF gives 4% escapers/DH (the *F two D1 chromosomes differ in extent of head defects -- DcxF in most *F genotypes has none, but they become dramatic over DH). D3/DH is, on *F the other hand, nearly completely viable (85-96% relative to the two *F balancer sib classes), with milder head defects (D3 itself has reverted *F that phene) but with missing bristles (not a dominant phene of D3, is a *F recessive phene of some other allele). Since D3 is still lethal over *F other alleles (eg, Sai1 and Sai2) it still carries a mutant allele of *F the 69D gene, but that allele is clearly different from the one carried on *F the parent D1 chromosome. # *U FBrf0098594 *a O'Kane *b C. *t 1997.9.17 *T personal communication to FlyBase *u *F >From c.okane@gen.cam.ac.uk Wed Sep 17 18:21:21 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 17 Sep 1997 18:21:21 +0100 *F X-Sender: cok@mole.bio.cam.ac.uk *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 17 Sep 1997 18:27:21 +0000 *F To: Michael Ashburner (Genetics) *F From: Cahir O'Kane *F Subject: Re: Your RpL3 gene *F Content-Length: 1156 *F >Cahir *F > *F >I have just updated yr FlyBase for your sequence record in RpL3. *F > *F >Do you have any information of the relationship - if any - of this *F >to M(3)86D ? *F No - apart from the obvious similarity in map position. a combination of *F Yong's cytology and Edwin's P1 colony lifts suggests 86D8-10 for RpL3. *F It's essentially been a part-time hobby of Edwin's, and I think that it *F would probably take too much effort to do something like sequence the gene *F in the single mutant allele available. If someone handed us an RpL3 *F antibody on a plate we could try a Western, but the chances of identifying *F a change with only one mutant allele probably aren't high. *F Regards, *F Cahir # *U FBrf0098595 *a Brand *b A. *t 1997.6.30 *T personal communication to FlyBase *u *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000021 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}h[1J3] *F chromosome: *F 3 *F within_gene: *F hairy (h) *F phenotype: *F recessive; extra scutellar bristles *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -hairy expression pattern (Brand and Perrimon [1993] Development *F 118, 401-415. *F date_updated: *F Jun 27 1997 3:13:41:623PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000023 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}24B *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Embryonic mesoderm from stage 10 (Brand and Perrimon [1993] *F Development 118, 401-415. *F -Eye-antennal, haltere, leg and wing imaginal discs. *F date_updated: *F Jun 27 1997 3:16:44:106PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000024 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}69B *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic ectoderm (stripes) from stage 11 *F -Eye-antennal, haltere, leg and wing imaginal discs (Brand and Perrimon *F [1993]; Development 118:401-415; Wilder and Perrimon [1995]; *F Development 121:477-488). *F date_updated: *F Jun 27 1997 3:09:45:360PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000025 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}32B *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B} target construct) *F expression: *F Embryonic ectoderm (stripes), stage 11. *F Eye-antennal, leg and wing imaginal discs (Brand and Perrimon [1993] *F 118, 401-419). *F date_updated: *F Jun 27 1997 3:45:49:483PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000026 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}71B *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands. *F -Part of dorsal and ventral, posterior wing pouch of wing imaginal *F discs (excluded from area around margin); very limited expression in *F eye-antennal imaginal discs (Brand and Perimon [1993] Development 118, *F 401-415. NOTE: Figure 9, C and D [not A and B, as described in *F legend]). *F -Expressed in two dorsal spots in the leg imaginal discs. *F date_updated: *F Jun 27 1997 3:58:48:156PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000027 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}30A *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F Embryonic salivary glands, muscle attachment sites. *F Dorsal sector of the leg imaginal disc. *F Primarily in the wing hinge region of the wing imaginal disc; dorsal *F and ventral edges of the eye imaginal disc; single, central dot in *F antennal disc (Brand and Perrimon [1993] Development 118, 401-415. *F NOTE: Figure 9A and B [not C and D as described in legend]. *F insert_comments: *F See also Ingham and Feitz [1995] Current Biology 5:432-440. *F date_updated: *F Jun 27 1997 4:25:15:253PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000028 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}34B *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Embryonic salivary glands. *F -Posterior midgut during embryogenesis (unconfirmed). *F -Eye-antennal, haltere, leg and wing imaginal discs (details *F undocumented). *F date_updated: *F Jun 27 1997 4:30:19:963PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000029 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}55B *F chromosome: *F 3 *F phenotype: *F May be a supressor of D-raf (J.A. Kiger, personal communication). *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F Embryonic salivary glands. *F Follicle cells (patchy expression) at anterior of oocyte at stage 9 *F (Brand and Perrimon [1994], Genes and Development 8:629-639). *F date_updated: *F Jun 27 1997 4:43:10:736PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000030 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e22c *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F 'Ubiquitous' in embryos. *F 'Ubiquitous' in imaginal discs. *F Ovaries (no details available). *F larval brain, third instar (no details available). *F insert_comments: *F This line was generated by A. Brand and K. Yoffe. *F date_updated: *F Jun 27 1997 5:41:12:383PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000031 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}T80 *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Said to be expressed 'ubiquitously' in the imaginal discs at third *F instar. *F insert_comments: *F -Line was isolated by J. Urban and G. Technau. *F -Insertion is thought to be viable, but this is unconfirmed. *F date_updated: *F Jun 27 1997 5:44:48:340PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000032 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e16e *F chromosome: *F 2 *F within_gene: *F not determined (thought to be in/near engrailed) *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F engrailed expression pattern. *F insert_comments: *F This insertion was generated by A. Brand and K. Yoffe *F date_updated: *F Jun 27 1997 5:49:32:596PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000034 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}10B *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -No embryonic expression. *F -In the leg imaginal disc there is a very small spot of expression at *F the very center of the disc. *F -In the haltere imaginal disc there are 2-3 very small spots of *F expression along the A/P border, including one at the center of the *F disc. *F -In the wing imaginal disc there are numerous small spots of expression *F on either side of the D/V border. May include some of the regions *F where SMC's arise, but does not appear to be restricted to the regions *F where the SMCs arise. *F -In the eye/imaginal disc there is a very small spot of expression at *F the posterior end of the antennal portion of the disc. *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 27 1997 6:06:46:436PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000035 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}56B *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B} target construct. *F expression: *F -Embryonic salivary glands and oenocytes. *F -In the leg imaginal disc there is broad expression throughout, with *F the possible exception of distal, ventral, posterior regions. *F -in the eye/antennal imaginal disc, there is broad expression nearly *F throughout. *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 27 1997 6:04:09:433PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000038 *F *F GawB: *F Yes *F insertion_symbol: *F P{GaWB}38B *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands, proventriculus. *F -At third larval instar, there is a single small dot of expression *F toward the center of the leg disc. *F -In the wing disc there are three small spots of expression in the *F dorsal hinge region and two larger spots on either side of the A/P *F border in the thoracic region *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 27 1997 6:17:38:256PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000039 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}36B *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic mesoderm form stage 10. *F -During third instar there is expression in the leg discs (no details *F available). *F -In the wing disc, there is intense expression in the thoracic region, *F but little or no expression in the wing hinge or wing pouch. *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 27 1997 6:24:52:276PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000040 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}33A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands. *F -At third instar, there is little or no expression in the eye/antennal, *F wing, leg, and haltere discs *F -There is expression in the 3rd instar larval brain/ventral ganglion *F (no further details available) *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 27 1997 7:06:08:186PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000042 *F *F date_updated: *F Jun 30 1997 12:51:14:316PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000043 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}6B *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands. *F -During third instar, there is expression in the wing (dorsal wing *F pouch), leg, and eye/antennal discs. In the eye portion of the disc *F there is expression at the dorsal and ventral edges. In the antennal *F portion of the disc, about half the disc shows expression (further *F details not available) *F insert_comments: *F Generated by A. Brand *F date_updated: *F Jun 30 1997 1:00:13:853PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000045 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}30B *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands, pharynx. *F -At third instar, there is expression throughout the wing pouch and *F thoracic regions of the disc, except in limited portions of the wing *F hinge region. There is broad expression in the antennal part of the eye *F disc and in parts of the eye disc itself. In the leg discs, only the *F proximal regions do not show expression. *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 30 1997 3:26:50:566PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000046 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}54B *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands, oenocytes. *F -At third instar, there is restricted expression in the leg, wing, *F haltere and eye/antennal imaginal discs (no details available). *F insert_comments: *F Generated by A. Brand. *F date_updated: *F Jun 30 1997 3:30:12:943PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000047 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}13M *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic epidermis. *F -At third instar, there is expression in the leg and eye/antennal *F imaginal discs (no other details available). *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 3:45:43:106PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000048 *F *F chromosome: *F *F date_updated: *F Jun 30 1997 3:48:00:243PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000049 *F *F chromosome: *F *F date_updated: *F Jun 30 1997 4:24:09:543PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000052 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}78B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands. *F -During third instar, there is no expression in the imaginal discs. *F -Ovaries. *F insert_comments: *F Generated by A. Brand *F date_updated: *F Jun 30 1997 4:53:31:873PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000053 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}28A *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands, oenocytes. *F -At third instar, there is no expression in the imaginal discs. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:05:23:983PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000054 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}79B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands. *F -At third instar, there is no expression in the imaginal discs. *F insert_comments: *F Generated by A. Brand *F date_updated: *F Jun 30 1997 5:09:04:370PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000055 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}17M *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands, yolk. *F -At third instar, there is no expression in the imaginal discs. *F -Larval brain (no further details available). *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:14:10:210PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000056 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}46B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands. *F -At third instar, there is no expression in the imaginal discs. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:22:28:983PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000057 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}53B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands, antenno-maxillary complex? *F -During third instar, no expression in imaginal discs. *F -Ovary. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:30:57:180PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000058 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}40B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands, muscle attachment sites?. *F -At third instar, there is no expression in the imaginal discs. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:34:12:810PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000059 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}58B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands. *F -At third instar, no expression in the imaginal discs. *F -Ovary. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:37:50:403PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000060 *F *F date_updated: *F Jun 30 1997 5:39:47:996PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000061 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}12B *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands, mesoderm. *F -At third instar, no expression in imaginal discs. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 5:44:42:226PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000063 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}11A *F visualization: *F via P{UAS-lacZ.B} target construct *F expression: *F -Embryonic salivary glands, dorsal median cells, dorsal cells at *F closure. *F -At third instar, no expression in imaginal discs. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 6:31:08:040PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000064 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e33C *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F During embryogenesis there is expression along the midline of the *F central nervous system (no further details available.) *F At third instar there is uniform expression in the lymph glands, *F as well as expression in the trachea, Malpighian tubules gut, *F optic lobes and brain (no further details available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:40:28:006PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000065 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e45A *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis there are epidermal/mesodermal stripes. *F (No further details available). *F -At third instar, there is expression in the eye/antennal and *F leg discs, and in the brain (no further details available). *F insert_comments: *F Generated by A. Brand and K. Yoffe. *F date_updated: *F Jun 30 1997 6:39:34:986PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000066 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e43A *F chromosome: *F 2 *F within_gene: *F engrailed (en) *F polytene: *F 48A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis this insertion is expressed in the 'late' *F engrailed expression pattern (i.e. there is no expression early in *F embryogenesis) *F -Expression during larval/pupal stages has not yet been described. *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:42:31:206PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000067 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e74A *F chromosome: *F 2 *F within_gene: *F engrailed (en) *F polytene: *F 48A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis this insertion is expressed in the 'late' *F engrailed expression pattern (i.e. there is no expression at early *F stage). *F -At third instar, expression has not yet been determined. *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:44:49:006PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000068 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e76B *F chromosome: *F 2 *F visualization: *F P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis there is expression in epidermal stripes and *F in the gut (no further details are available). *F -At third instar, there is little or no expression in the imaginal *F discs. *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:46:15:406PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000069 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}eIT *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis there is expression in the mesoderm and in *F the central nervous sytem (no further details available). *F -Expression during third instar has yet to be determined. *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:47:56:606PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000070 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e6A *F visualization: *F P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis there is expression in the central nervous *F system (no further details available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:49:33:626PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000071 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e1A *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -There is expression during embryogenesis (no further details *F available). *F -At third instar, there is 'ubiquitous' expression in the imaginal *F discs, and expression in the brain (no further details available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:52:08:626PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000072 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e16A *F chromosome: *F 2 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F During embryogenesis there is expression in the central *F nervous system (no further details available). *F -At third instar there is little or no expression in the imaginal *F discs, but there is expression in the brain (no further details *F available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:54:04:226PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000073 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e29C *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis there is expression in the central nervous *F system and in the head (no further details are available). *F -At third instar this insertion is 'ubiquitiously' expressed in *F the imaginal discs and is also expressed in the brain (no further *F details available.) *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:56:07:226PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000074 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e15A *F chromosome: *F 2 *F within_gene: *F engrailed (en) *F polytene: *F 48A *F visualization: *F via P{UAS-lacZ.B)target construct *F expression: *F -During embryogenesis this insertion is expressed in the 'late' *F engrailed expression pattern (i.e. there is no expression early in *F embryogenesis). *F -At third instar there is expression in the eye/antennal, haltere, *F wing and leg imaginal discs (no details available), and in the *F larval brain (no details available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 6:57:34:626PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000075 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e21A *F chromosome: *F 2 *F within_gene: *F engrailed (en) *F polytene: *F 48A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis this insertion is expressed in the 'late' *F engrailed expression pattern (i.e. there is no expression early in *F embryogenesis). *F -At third instar this insertion is expressed in the endogenous *F engrailed pattern in the imaginal discs, and is expressed in the *F brain (no details available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 7:00:47:646PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000076 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e33A *F chromosome: *F 2 *F within_gene: *F engrailed (en) *F polytene: *F 48A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis this insertion is expressed in the 'late' *F engrailed expression pattern (ie there is no expression early in *F embryogenesis). *F -At third instar there are 'spots' of expression in the posterior *F compartment of the imaginal discs. There is also expression in the *F larval brain (no details available). *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 7:06:01:046PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000077 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e8A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -During embryogenesis there is expression in the central nervous *F system (no further details available) *F -At third instar there is expression in the cuticle, gut and brain *F (no further details available) *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 7:07:43:066PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000078 *F *F GawB: *F No *F transposon: *F P{en-GAL4} *F transposon_construct: *F Enhancer trap vector, generated by A. Brand. *F engrailed upstream sequence ligated to GAL4 coding sequence. *F insertion_symbol: *F P{en-GAL4}e13C *F chromosome: *F 3 *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -There is expression during embryogenesis (no further details *F available). *F At third instar, there are 'spots' of expression in the posterior *F compartment of the imaginal discs, as well as in the salivary glands *F fat bodies, Malpighian tubules and gut (no further details available) *F insert_comments: *F Generated by A.Brand and K.Yoffe. *F date_updated: *F Jun 30 1997 7:09:23:666PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000079 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}L *F chromosome: *F 1 *F expression: *F Embryonic salivary glands. *F insert_comments: *F This insertion was used as the 'starter' line for generating novel *F insertions. *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 7:12:21:043PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000080 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}19A *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands, epidermal stripes. *F -Third instar, expression anterior to the morphogenetic furrow in *F eye/antennal disc, in central ring of the antennal disc. *F In leg disc, expression in a central ring, a large dorsal patch, small *F dots scattered throughout an external ring (proximal leg fates). *F In wing discs, four patches of expression along the A/P border, at *F least 4 patches elsewhere in the wing region, and four patches in the *F thoracic region of the disc. *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 7:19:58:886PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000081 *F *F date_updated: *F Jun 30 1997 7:21:34:443PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000082 *F *F GawB: *F Yes *F insertion_symbol: *F P{GawB}76B *F visualization: *F via P{UAS-lacZ.B}target construct *F expression: *F -Embryonic salivary glands, ring gland?. *F -At third instar there is strong pattern expression in the lymph *F glands as well as expression in the salivary glands, trachea, optic *F lobes, brain, and imaginal discs (no further details available). *F insert_comments: *F Generated by A.Brand. *F date_updated: *F Jun 30 1997 7:28:50:703PM *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000094 *F *F transposon: *F P{UAS-Dras2 Val14} *F vector: *F pP{UAST} *F genomic: *F Ras2, Val14 *F vector_comments: *F Generated by A.Brand, described in Brand and Perrimon (1993) *F Development 118, 401-415. *F date_updated: *F Jun 30 1997 7:49:43:760PM *F *F ===== *F insertion_symbol: *F P{UAS-Dras Val14}B *F chromosome: *F 2 *F insertion_property: *F viable *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000095 *F *F transposon: *F P{UAS-cRaf1.gof} *F vector: *F pP{UAST} *F other_regulatory: *F human cRaf1, deletion aa 2-334 (constitutively active) *F vector_comments: *F Generated by A. Brand, described in Brand and Perrimon (1994) Genes *F and Development 8, 629-639. *F date_updated: *F Jun 30 1997 7:57:39:100PM *F *F ===== *F insertion_symbol: *F P{UAS-cRaf1.gof}X *F chromosome: *F 1 *F insertion_property: *F viable *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000096 *F *F transposon: *F P{UAS-raf.gof} *F vector: *F pP{UAST} *F cDNA: *F raf, deletion aa 2-431 (constitutively active) *F vector_comments: *F Generated by X.Lu, described in Brand and Perrimon (1994) Genes and *F Development 8, 629-639. *F date_updated: *F Jun 30 1997 8:04:51:366PM *F *F ===== *F insertion_symbol: *F P{UAS-raf.gof}F179 *F chromosome: *F 3 *F insertion_property: *F viable *F -------------------------------------------------- *F *F lastname : Brand *F givenname : Andrea *F institution : Wellcome/CRC Institute *F email : ahb@mole.bio.cam.ac.uk *F FBgu ID : FBgu0000099 *F *F transposon: *F P{UAS-lacZ} *F vector: *F pP{UAST} *F other_regulatory: *F Bacteria, lacZ *F vector_comments: *F Generated by A. Brand, described in Brand and Perrimon (1993) *F Development 118, 401-415. *F date_updated: *F Jun 30 1997 8:09:20:446PM *F *F ===== *F insertion_symbol: *F P{UAS-lacZ}Bg4-1-2 *F chromosome: *F 2 *F insertion_property: *F viable *F *F ===== *F insertion_symbol: *F P{UAS-lacZ}Bg4-2-4b *F chromosome: *F 3 *F insertion_property: *F viable # *U FBrf0098597 *a Zhimulev *b I.F. *c E.I. *d Volkova *e V.F. *f Semeshin *g E.S. *h Belyaeva *t 1997.9.29 *T personal communication to FlyBase *u *F >From zhimulev@ghost.bionet.nsc.ru Mon Sep 22 08:01:10 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 22 Sep 1997 08:01:10 +0100 *F Comments: Authenticated sender is *F From: 'Zhimulev I.F.' *F Organization: Institute of Cytology and Genetics *F To: ma11@gen.cam.ac.uk *F Date: Thu, 18 Sep 1997 20:18:04 +0600 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=US-ASCII *F Content-transfer-encoding: 7BIT *F Subject: stocks *F Reply-to: zhimulev@bionet.nsc.ru *F X-Confirm-Reading-To: zhimulev@bionet.nsc.ru *F X-pmrqc: 1 *F Return-receipt-to: zhimulev@bionet.nsc.ru *F Priority: normal *F X-mailer: Pegasus Mail for Windows (v2.52) *F Content-Length: 2354 *F Mutation Notes - D. melanogaster. *F Zhimulev I.F., Volkova E.I., Semeshin V.F., Belyaeva E.S. *F Institute Cytology and Genetics, Novosibirsk, 630090, Russia *F New cytological data on some chromosome rearrangements in D. *F melanogaster. *F Aberration Data in FlyBase New cytology *F Df(2R)E3363 47A; 47F 47A3; 47D12 *F Df(2R)Stan1 46D7-9; 47F15-16 44A1-2; 47F *F Df(2R)Stan2 46F1-2; 47D1-2 46F1; 47B9 *F Df(2R)X-58-5 58B1-2; 59A1 58B3; 58F8 *F Df(2R)X58-6 58A3-B2; 58E3-10 58A3-4; 58E1-4 *F Df(2R)X58-7 58A1-2; 58E4-10 58B1-2; 58E1-4 *F Df(2R)X58-8 58A1-2; 58F3-5 58B3; 59A1 *F Df(2R)X58-11 58A3-4; 58E3-7 58B1-2; 58E1-4 *F Df(3L)Aprt 14 - 62B3; 62E1-2 *F Df(3L)Aprt 27 62B5-7; 62D6-E1 62B6; 62D7 *F Df(3L)Aprt 32 62A2-B1; 62E6-F1 62B1-2; 62E3 *F Df(3L)Aprt 65 62B8-9; 62D6-E4 62B3; 62D4 *F Df(3L)Aprt 72 62B2-4; 62D6-E4 62B6; 62E7 *F Df(3L)Aprt 105 62B2-4; 62D6-E1 62B3; 62E1-2 *F Df(3L)Aprt 112 62A10-B1; 62D6-E1 62B2; 62E1-2 *F Df(3L)Aprt 113 62B8-9; 62D6-E2 62B11; 62E2 *F Df(3L)Aprt 123 - 62B4-5; 62E1-2 *F Df(3L)Aprt 124 62B8-9; 62D6-E4 62B9; 62E1-2 *F Df(3L)Aprt 144 - 62C1-2 *F Df(3L)Aprt 148 62A7-B1; 62E6-F1 62B11; 62E1-2 *F Df(3L)Aprt 156 62B2-4; 62E6-F1 62B6; 62E9 *F Df(3L)Aprt 166 - 62B9; 62E1-2 *F Df(3L)Aprt 175 62B8-9; 62D6-E4 62B6; 62E1-2 *F Df(3L)Aprt 177 62B2-4; 62D6-E1 62B9; 62E2 *F Df(3L)Aprt 185 - T(2;3)62B9; 49D *F Df(3L)BK10 71E1-2; 71F4-5 71C3; 71E5 *F Df(3L) brm11 71F1-4; 72D1-10 72A3-4; 72C1 *F Df(3L) D-5rv14 70C2-3; 72A 70D-E1-2;71C1-2 + *F In(3L) 71C3; 72D1-2 *F Df(3L)GN19 63E6-9; 64B2-4 63F3; 64A12 *F Df(3L)GN34 63F7; 64A7-B2 63E6; 64A10 *F Df(3L)GN50 63E1-2; 64B17 63E2-3; 64B17 *F Df(3L)HR119 63C6; 63E 63C2; 63F7 *F Df(3L)R-G7 62B8-9; 62F2-5 62B9; 62E7 *F Df(3L) th102 71F3-5; 72D12 72A2; 72E12 *F Dear Dr. Zhimulev, *F Thank you for the data for FlyBase that you sent to Michael. I am just now *F curating it, to incorporate it into our files. *F I have a two questions. *F Firstly, you wrote: *F 'Df(3L)Aprt 185 - T(2;3)62B9; 49D' *F Are you saying that this 'Df' is actually a translocation? We already have *F data that suggests it deletes 15 loci, which would be difficult to explain *F if it were indeed a translocation. It could of course be a deficiency as *F well as a translocation, in which case it should have three breakpoints. *F Can you shed any light on the situation? *F Secondly, you wrote *F 'Df(3L) D-5rv14 70C2-3; 72A 70D-E1-2;71C1-2 + *F In(3L) 71C3; 72D1-2' *F The data in our files suggest that Df(3L)D-5rv12 is a deletion, with breaks *F 70C2 and 72A1, superimposed on the progenitor chromosome, Df(3L)D5, with *F breaks 70D2 and 70D4. *F Are you suggesting that Df(3L)D5 was actually an inversion between 71C3 *F and 72D1-2 and that Df(3L)D-5rv14 then deleted 70D-E2 to 71C1-2? Or vice *F versa (more likely, from the Dichaete point of view). *F Many thanks for your help. *F with best wishes, *F Rachel. *F From zhimulev@ghost.bionet.nsc.ru Tue Sep 30 08:22:27 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 30 Sep 1997 08:22:27 +0100 *F Comments: Authenticated sender is *F From: 'Zhimulev I.F.' *F Organization: Institute of Cytology and Genetics *F To: rd120@gen.cam.ac.uk *F Date: Tue, 30 Sep 1997 00:33:41 +0600 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=US-ASCII *F Content-transfer-encoding: 7BIT *F Subject: Re: Helping FlyBase *F Reply-to: zhimulev@bionet.nsc.ru *F X-Confirm-Reading-To: zhimulev@bionet.nsc.ru *F X-pmrqc: 1 *F Return-receipt-to: zhimulev@bionet.nsc.ru *F Priority: normal *F X-mailer: Pegasus Mail for Windows (v2.52) *F Content-Length: 458 *F Dear Dr. Drysdale, *F thank you for your e-mail message. I conform that Df(3L)Aprt185 *F was found to be T(2;3)62B9; 49D. At least at the stock we *F received from authors. As for Df(3L) D-5rv14 I can say that it *F is deficiency with inversion in this region 70D-E1-2;71C1-2+ *F In(3L) 71C3; 72D1-2 I can say that it is deficiency with *F inversion in this region. Deficiency goes first and then goes *F inversion. *F Thank you very much for your attention. Igor Zhimulev. # *U FBrf0098598 *a Carpenter *b A.T.C. *t 1997.9.30 *T personal communication to FlyBase *u *F >From atc12@mole.bio.cam.ac.uk Mon Sep 29 15:13:22 1997 *F Subject: Revised cytology of Df(2L)DS* *F Revised cytology of DFs(2L)DS's. Base of 2L revised between Bridges low *F and high mag maps; in addition to not noting this, Lefevre made an error *F in assigning 40A1,2. I have checked my interpretation of 38-40 on Bridges *F high-mag map with Sorsa and we agree as to which band is which. All of *F these deficiencies \*were* induced on an isogenic b pr cn chromosome. *F Df(2L)DS5 (stock via David Nash) 38C7-10;39D3-E1 *F (right breakpoint agrees with Moore et al assuming map shift; *F left breakpoint does not) *F Df(2L)DS8 (stock via David Huen, presumably from Ashburner collection) *F 38E10-F3;39B3-C4 *F (both breakpoints agree with Moore et al assuming map shift) *F Df(2L)DS9 (stock via David Nash) 38C7-D2;39A1-3. Fusion band probably *F contains distal 38D1.2 plus proximal 39A1-3 *F (right breakpoint agrees with Moore et al assuming map shift; *F left breakpoint does not) *F The unattributed 'pr-' for DS5 comes from Lindsley and Zimm; given that *F the chromosome was already pr1 I think we can assume this was a *F stock-label error of someone lost in the mists of history. *F Adelaide TC Carpenter # *U FBrf0098604 *a Mistry *b H. *t 1997 *T personal communication to FlyBase *u *F From h.mistry@gen.cam.ac.uk Wed Oct 01 14:57:49 1997 *F Dear Rachel, *F I have renamed l(2)35Cd, guftagu (gft). 'guftagu' means 'private *F conversation' in Urdu. Mutations in gft suppress the phenotype caused by *F ectopic expression of activated G-alpha s. gft and G-alpha s regulated *F signalling pathways appear to be having a private conversation that I have *F 'eavesdropped' upon. *F Hemi. # *U FBrf0098700 *a Coen *b D. *t 1997.8.27 *T personal communication to FlyBase *u *F From dario.coen@emex.u-psud.fr Wed Aug 27 13:26:15 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 27 Aug 1997 13:26:15 +0100 *F X-Sender: coen@psisun.u-psud.fr *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 27 Aug 1997 14:38:47 +0200 *F To: Rachel Drysdale (Genetics) *F From: Dario Coen *F Subject: Re: Helping FlyBase crep and oap *F Content-Length: 553 *F *F Dear Rachel, *F I am not sure to understand your question. *F The name we gave to the alleles in the abstract were crep[1], *F crep[2]...etc and oap[1]. These name are no longer in use. I have to *F mention that what we called crep[2] is l(3)A5-3-42. *F I hope this will help *F Best *F Dario # *U FBrf0098701 *a Campbell *b S. *t 1997.3.15 *T personal communication to FlyBase *u *F The information regarding the following P inserts is misleading. They *F are the closest detectable insertions to the Dwee1 coding region, but *F there is no evidence that they are in fact alleles of Dwee1. *F *F P{PZ}wee[02647] *F P{PZ}wee[10280] *F P{PZ}wee[rF680] *F P{lacW}wee[k10413] *F P{lacW}wee[k06203] *F *F Thank you, *F *F Shelagh Campbell *F Dept of Biochemistry, UCSF, San Francisco # *U FBrf0098925 *a Mohler *b J. *t 1997.8.7 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Jym Mohler, Barnard College *F To: Bloomington Drosophila Stock Center *F Subject: Df(2R)knSA3 *F Dated: 7 August 1997 *F *F Information communicated: *F *F Df(2R)knSA3, 51B5-11;51F5-13, deletes kn. # *U FBrf0098931 *a Matthews *b K. *t 1997.10.8 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Coa[1] phenotype *F Date: 8 October 1997 *F *F Information communicated: *F Coa[1] produces short, thickened hairs on the surface of the wing blade, resulting in a leathery look to the wings. The hairs along the wing margin appear normal. Other hairs were not examined. # *U FBrf0098936 *a Gieseler *b K. *c J. *d Pradel *e A. *f Martinez Arias *t 1997.10.13 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Mon Jul 28 15:24:34 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 28 Jul 1997 15:24:34 +0100 *F To: ama11@cus.cam.ac.uk *F Subject: Help FlyBase - wg-GAL4 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 28 Jul 1997 15:29:26 +0100 *F Content-Length: 445 *F Hi Alfonso, *F I am a curating a paper for FlyBase: *F Glise and Noselli, 1997, Genes Dev. 11(13): 1738 *F in which they discuss wg-GAL4 provided by you. *F We do not have this constructs in FlyBase so could you please provide *F molecular details of the construct (and any references of there are *F any). I have also queried Stephane for this construct also but I thought *F there was no harm in asking you too! *F Thanks for your help, *F Eleanor Whitfield *F FlyBase *F From ama11@cus.cam.ac.uk Mon Oct 13 12:17:52 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 13 Oct 1997 12:17:52 +0100 *F Date: Mon, 13 Oct 1997 12:24:09 +0100 (BST) *F From: 'A. Martinez-Arias' *F To: Eleanor Whitfield *F Subject: Re: Help FlyBase - wg-GAL4 *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 501 *F The piece that you want to know about is 7.8kb of 5' untranslated control *F region of wg. This DNA provides all (ALL) functions for the embryonic *F pattern and contains only a very small and not clear pattern from the *F adult. *F Let me know if this is OK *F Alfonso # *U FBrf0098953 *a Maroy *b P. *t 1997.10.17 *T personal communication to FlyBase *u *F From maroy@sol.cc.u-szeged.hu Fri Oct 17 07:39:59 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Fri, 17 Oct 1997 07:39:59 +0100 *F Date: Fri, 17 Oct 1997 08:45:44 +0200 *F X-Sender: maroy@sol.cc.u-szeged.hu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Aubrey de Grey *F From: maroy@sol.cc.u-szeged.hu (Peter Maroy) *F Subject: Re: list *F X-Mailer: *F Content-Length: 823 *F Dear Aubrey, *F ZP1 is a deletion we isolated in my lab by Peter Zavorszky. This is a *F deletion with brake points 66A17-20;66C1-5. The mutagen is X-ray, and *F actually was isolated by marker loss irradiating the chromosome carrying one *F of Issing's TE insertion, the TE198. ZP1 is not complementing TE198, DTS4, *F and Df(3L)pblX1, Df(3L)66C-G28. ZP1 is not published so far. *F Well this is what I know. *F Cheers, *F Peter *F >Hi: *F > *F >I've been checking the deficiencies you used and there is one I can't *F >trace: 'ZP1(66A;66C)' Where did you get this Df? Any info that may *F >help me to trace it would be very helpful. *F > *F >Cheers, Aubrey *F > *F > *F Peter Maroy, Department of Genetics *F A.Jozsef University, Kozepfasor 52., H-6726 Szeged, Hungary *F email: MAROY@SOL.CC.U-SZEGED.HU *F tel:36-62-454025, fax:36-62-432485 # *U FBrf0099001 *a Prout *b M. *t 1997.3.19 *T personal communication to FlyBase *u *F Archived. # *U FBrf0099036 *a Omelyanchuk *b L. *t 1997.10.10 *T personal communication to FlyBase *u *F From ome@ghost.bionet.nsc.ru Fri Oct 10 04:07:45 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 10 Oct 1997 04:07:45 +0100 *F Comments: Authenticated sender is *F From: 'Emeljanchuk L.V.' *F Organization: Institute of Cytology and Genetics *F To: Rachel Drysdale (Genetics) *F Date: Fri, 10 Oct 1997 10:16:32 +0600 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset=US-ASCII *F Content-transfer-encoding: 7BIT *F Subject: inform *F Return-receipt-to: 'Emeljanchuk L.V.' *F Priority: normal *F X-mailer: Pegasus Mail for Windows (v2.54) *F Content-Length: 167 *F Dear Dr Drysdale *F In addition to the mutants described we discover an insertion of *F PlArB 36w showing partial female sterility and map it in 28A-B. *F L Omelyanchuk # *U FBrf0099037 *a Mozer *b B. *t 1997.11.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Oct 16 13:47:34 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 16 Oct 1997 13:47:34 +0100 *F To: bmozer@codon.nih.gov *F Subject: Helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F From: Rachel Drysdale (Genetics) *F Date: Thu, 16 Oct 1997 13:53:49 +0100 *F Content-Length: 1684 *F Dear Brian, *F I am curating the abstracts of the 1997 CSH Drosophila Neurobiology *F meeting, for the FlyBase genes file, and have a question for you about a *F symbol. *F On p133 you mention 'drFA30'. Is this an allele of Dr? dr is actually *F the symbol for the droopy locus, so I imagine you meant DrFA30. *F Is 'drFA30' related in any way to the l(3)FA30 gene? It looks like it *F may be the same thing from the symbol and map location. Here is our gene *F record for l(3)FA30: *F \*a l(3)FA30 *F \*x FBrf0080077 == Hamilton et al., 1995, Roux Arch. dev. Biol. 204(3): 187--192 *F \*c 99A7--99A7 *F \*c Left limit from molecular mapping relative to Ptp99A (FBrf0080077) *F \*c Right limit from molecular mapping relative to Ptp99A (FBrf0080077) *F \*z FBgn0020528 *F \*E FBrf0080077 == Hamilton et al., 1995, Roux Arch. dev. Biol. 204(3): 187--192 *F \*s Gene order: Overall orientation not stated: Ptp99A+ l(3)FA30? *F \*A l(3)FA30FA30 *F \*x FBrf0080077 == Hamilton et al., 1995, Roux Arch. dev. Biol. 204(3): 187--192 *F \*z FBal0061257 *F \*E FBrf0080077 == Hamilton et al., 1995, Roux Arch. dev. Biol. 204(3): 187--192 *F \*i FA30 *F \*k Phenotypic class: lethal | recessive *F \*o P-element activity *F \*G P{lacW}l(3)FA30FA30 *F \*O P{lacW}Y603 *F I'll curate your reply as a personal communication to FlyBase. *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From bmozer@codon.nih.gov Thu Oct 16 16:22:39 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 16 Oct 1997 16:22:39 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Thu, 16 Oct 1997 10:36:30 -0500 *F To: Rachel Drysdale (Genetics) *F From: bmozer@codon.nih.gov *F Subject: Re: Helping FlyBase *F Content-Length: 505 *F Dear Rachel, *F Yes, FA30 is an allele of Drop but recessive which is why I noted it as *F lower case. I didn't know there was a droopy locus so I guess I can't use *F the normal convention of upper case for dominant and lower case for the *F recessive. Do you have any suggestions? *F Regarding the FA30 P-element insertion isolated by Hamilton, yes this is *F what I was referring to however the insertion of the P element in this line *F is not the cause of the lethality, nor the Drop noncomplementation. *F Brian Mozer # *U FBrf0099038 *a Rooke *b J. *t 1997.11.3 *T personal communication to FlyBase *u *F On Mon, 3 Nov 1997, Rachel Drysdale wrote: *F > *F > Hi Jenny, *F > What a great story about kuzbanian. I'm writing to ask whether you would *F > mind me curating your bionet.drosophila posting for FlyBase. We like to *F > include etymology of gene names wherever we can, and I can treat your bionet *F > posting as a personal communication to FlyBase from you, if you like. *F > best wishes, *F > Rachel. *F Rachel, *F You're certainly welcome to include my posting in FlyBase. *F And I believe it will help reduce confusion surrounding my gene name *F choice. Thanks for asking! *F Jenny *F > Mail*Link=AE SMTP FWD>kuzbanian *F >=20 *F > Hello, does anybody know what is the meaning of kuzbanian (the name of a *F > new neurogenic gene) and in which language? *F >=20 *F > thank you very much *F The name 'kuzbanian' is a reference to members of an alien lifeform (?) of *F Muppets who appeared in about 4 episodes of the original Muppet Show by *F Jim Henson. The Koozbanians (gene spelling changed to avoid copyright *F infringement) dwell on the planet Koozbane and speak Koozbanese. They also *F have wildly uncontrollable hair sticking up on the tops of their heads, *F reminiscent of the striking supernumerary bristle phenotype in *F Drosophila mosaic for a null kuzbanian mutation -- and there's the *F derivation of the gene name. *F Jenny Rooke *F Yale Dept. of Genetics # *U FBrf0099039 *a Lin *b D. *t 1997.11.4 *T personal communication to FlyBase *u *F Hi Dave, *F I'm writing about a paper I am curating for FlyBase. *F Davis et al., Neuron 19: 561 *F Genetic analysis of the mechanisms controlling target selection: *F target-derived Fasciclin II regulates the pattern of synapse formation. *F In their materials and methods they describe two things made by you: *F UAS-FasII (PEST+) *F and *F UAS-B-galrep6 *F We have one UAS-Fas2 construct from you but it is not the same one (record *F included at the end, for your info). It would be marvellous if you could *F give me a line or two about its construction, then I can do a decent job of *F introducing the PEST+ version to FlyBase. I'll curate your reply as a *F personal communication to FlyBase. *F The same goes for the UAS-lacZ. *F Many thanks for your help, *F Rachel. *F From davelin@coreys.berkeley.edu Tue Nov 04 20:50:30 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 4 Nov 1997 20:50:30 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-Mailer: Eudora Pro 3.0 for Macintosh *F Date: Tue, 4 Nov 1997 13:02:18 -0800 *F To: rd120@gen.cam.ac.uk *F From: dave lin *F Subject: flybase *F Content-Length: 1538 *F Hi Rachel- *F UAS-rep6 (lacZ reporter construct) *F I did not make this construct. It was originally made by Janice Fischer *F and is described in her paper Nature, 1988 Apr 28, 332(6167):853-6. The *F parent UAS-type vector was created by Janice to see if GAL4 works at all in *F flies (the grandfather of all fly GAL4 papers), and is not the same as *F Andrea Brand's UAST construct. *F UAS-FasII PEST+ *F This was a little more complicated. I used a FasII PEST+ cDNA that Gregg *F Helt in Corey's lab had isolated from a cDNA library. I first modified the *F ends of the cDNA to remove all 5' and 3' untranslated sequences. At the 5' *F end: Not I, Xba I, AGA TAA AA ATG..... At the 3' end: TAA GG EcoRV *F HindIII. To create the UAST-FasII (PEST+) construct, the UAST vector was *F first digested with Asp718 and the 5' overhangs filled in with Klenow. It *F was then digested with NotI and CIAP treated. The FasII cDNA was cut with *F HindIII, the overhangs were filled in, and subsequently digested with NotI. *F The two fragments were then ligated together and the construct transformed. *F hope all of this is helpful! *F dave *F Dave Lin phone: 510-642-9887 *F 265 LSA FAX: 510-643-6791 *F UC Berkeley CA 94720 *F e-mail: davelin@coreys.berkeley.edu # *U FBrf0099060 *a Noll *b E. *t 1997.12.3 *T personal communication to FlyBase *u *F From crosby@morgan Mon Dec 1 11:37:24 1997 *F Date: Mon, 1 Dec 1997 11:37:16 -0500 *F From: crosby@morgan (Madeline Crosby) *F To: perrimon@rascal.med.harvard.edu *F Subject: GAL4 construct *F Cc: crosby@morgan *F X-Sun-Charset: US-ASCII *F Content-Length: 358 *F Norbert, *F FlyBase needs a bit more information concerning the GAL4 construct or *F insertion called 'rhlGAL4' in Axelrod, et al., 1996, Science 271:1826. *F Is this an insertion of P{GawB}? A GAL4 construct? If so, with regulatory *F sequences from what gene? To what does 'rhl' refer? *F Thanks for any enlightenment you can provide! *F \--Lynn Crosby *F FlyBase *F From noll@rascal.med.harvard.edu Wed Dec 3 14:26:15 1997 *F Date: Wed, 3 Dec 1997 14:26:15 -0500 *F Mime-Version: 1.0 *F To: crosby@morgan.harvard.edu *F From: noll@rascal.med.harvard.edu (Beth Noll) *F Subject: rhl gal4 *F Lynn, *F The rhl-gal4 is an pGawB insertion that was mapped by in situ to polytenes *F to the region where rhomboid lies. Based upon this, and the fact that it *F is expressed in the same pattern as rho, it was given the name *F 'rhomboid-like gal4' or 'rhl-gal4'. It is a viable insertion, and so it *F has likely not landed in the rhomboid gene itself, so we couched our naming *F of it to reflect that it's near rho, and is expressed like rho. *F I hope this info. is what you need. *F BN *F Elizabeth Noll *F Harvard Medical School-HHMI *F Department of Genetics *F Perrimon Lab *F 200 Longwood Ave. *F Boston, MA 02115 *F ph: (617)432-7571 *F fax: 432-7688 *F noll@rascal.med.harvard.edu # *U FBrf0099128 *a Bloomington Drosophila Stock Center *b ?. *t 1997.11.18 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: P{lArB}A215.1M3 and l(3)A215.1M3[A215.1M3] *F Date: 18 November 1997 *F *F Background: P{} insertion and lethal at Bloomington that is not in FlyBase. *F *F Information communicated: *F *F P{lArB}A215.1M3, l(3)A215.1M3[A215.1M3] was generated in the Gehring lab (Universitat Basel). Colocalization of P{} and lethality *F have not been tested; the aberration may or may not cause lethality. The site of the insertion is unknown. lacZ expression pattern in *F adult ovary: germarium (region 3), nurse cells (stage 10) (per Gehring lab). # *U FBrf0099129 *a Bloomington Drosophila Stock Center *b ?. *t 1997.11.20 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: T(3;4)Dubinin *F Date: 20 November 1997 *F *F Background: Translocation from the Mid-America stock collection, now at Bloomington, that is not in FlyBase. *F *F Information communicated: *F Aberration symbol T(3;4)Dubinin *F Comment from Mid-America stock list 'separated arms of 3 - T3L. 4L;4R. 3R' *F *F KM's comments: above seems to imply 3 centromere with 3L component and 4 centromere with 3R component, but I doubt that that is known. # *U FBrf0099171 *a Csink *b A. *t 1997.12.9 *T personal communication to FlyBase *u *F >From rd120@gen.cam.ac.uk Tue Nov 18 10:48:22 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 18 Nov 1997 10:48:22 +0000 *F To: steveh@howard.fhcrc.org *F Subject: Helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F From: Rachel Drysdale (Genetics) *F Date: Tue, 18 Nov 1997 10:55:12 +0000 *F Content-Length: 1040 *F Hi Steve, *F how are you? Well I hope. *F I'm writing about your latest Genetics paper: *F Martin-Morris et al, Genetics 147: 671--677 *F Heterochromatic trans-inactivation of white .... *F on page 674, column 1, under 'mini-white transgenes can be inactivated' it says *F 'These (three) transposon insertions are alleles of the Lighten-up gene *F (A.K. Csink, unpublished results).' *F I would like to know the allele symbols for these Lip mutations, so that we *F can record the P{lacW} insertions with symbols that will match their future *F publications, and so avoid duplicate entries in FlyBase. *F Many thanks for your help, *F with best wishes, *F Rachel. *F From acsink@fred.fhcrc.org Tue Dec 09 18:13:09 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 9 Dec 1997 18:13:09 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 9 Dec 1997 10:23:11 -0800 *F To: Rachel Drysdale (Genetics) *F From: acsink@fred.fhcrc.org (Amy Csink) *F Subject: Re: Helping FlyBase *F Content-Length: 802 *F Rachel, *F I'm responding (late, I apologized) to your e-mail to Steve Henikoff *F concerning the Lighten-up alleles described in our recent Genetics paper *F (October). I'm not certain how to designate the alleles. I received the *F insertions from the Berkeley Genome project and defined them as Lip alleles *F because they are 1) in the correct region 2) are lethal over other Lip *F alleles I've isolated and 3) dominantly enhance the wbl mutation. Is *F there any preferred standard for naming alleles from insertions collected *F by the the genome project? If not I would call them Lip3D2, Lip3D5 and *F Lip14E8. *F Sincerely, *F Amy Csink *F e-mail: acsink@fred.fhcrc.org *F Fred Hutchinson Cancer Research Center *F 1100 Fairview Avenue N, A1-162 *F P.O. Box 19024 *F Seattle, WA 98109-1024 USA *F (206)667-4509 *F (206)667-5889 (FAX) *F From rd120@gen.cam.ac.uk Wed Dec 10 09:15:28 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 10 Dec 1997 09:15:28 +0000 *F To: acsink@fred.fhcrc.org *F Subject: Re: Helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F From: Rachel Drysdale (Genetics) *F Date: Wed, 10 Dec 1997 09:22:42 +0000 *F Content-Length: 1251 *F Hi Amy, *F Thanks for your mail. *F >I received the *F >insertions from the Berkeley Genome project *F ah ha. Very interesting. I guess that the three alleles you have *F correspond to these three alleles of the gene FlyBase currently has as *F 'l(3)01086': *F l(3)01086j3D2 *F l(3)01086j3D5 *F l(3)01086j14E8 *F We prefer to keep the genome project allele designations, so we will rename *F this gene as Lip, using your message as the personal communication that *F indicated the identity of l(3)01086 with Lip, with the alleles being *F Lipj3D2 *F Lipj3D5 *F Lipj14E8 *F How's that? These are very close to your preferred symbols, and in line *F with the way we generally treat genome project alleles. *F Thankyou for the information. Its great when we can merge the rather *F anonymous genome project alleles with phenotypically exciting ones! *F with best wishes, *F Rachel. # *U FBrf0099222 *a Gelbart *b W. *t 1997.12.4 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Thu Dec 04 18:02:27 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Thu, 4 Dec 1997 18:02:27 +0000 *F Date: Thu, 04 Dec 1997 13:06:55 -0500 *F From: gelbart@morgan.harvard.edu (William Gelbart) *F Subject: Re: lynn.update.120397 *F To: crosby@morgan.harvard.edu *F Cc: curators@morgan.harvard.edu *F Content-transfer-encoding: 7BIT *F Content-Length: 879 *F dpp+t8 rescues only the embryonic phenotype. In a dpp null background, *F dpp+t8 animals die as larvae (shortvein region lethal phenotype). *F dpp+t20 rescues both the embryonic and shortvein region lethal phases, *F and in a null background, animals die during pupation with a dpp disk *F class V mutant phenotype. *F Bill # *U FBrf0099314 *a Kerridge *b S. *t 1997.11.26 *T personal communication to FlyBase *u *F From kerridge@ibdm.univ-mrs.fr Wed Nov 26 11:58:15 1997 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 26 Nov 1997 11:58:15 +0000 *F Date: Wed, 26 Nov 1997 13:04:03 +0100 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-Mailer: Eudora Light F3.1l *F To: Michael Ashburner (Genetics) *F From: kerridge *F Subject: Re: tsh3 *F Content-Length: 1086 *F Michael *F tsh3 was induced on an enhancer trap line (P(lac, ry+)A from *F O'Kane/Gehring) localized at the 5' end of tsh. The tsh3 revertant is a ry- *F derivative of the original ry+ P insertion. If things are simple the breaks *F should be in 34Db and the ry gene. Primers could therefore be tried from *F the 3' P element sequence or whatever (ask Cahir?) is at that end of the *F insertion from the ry gene. *F Let me know if you need more help. *F Steve *F Stephen Kerridge *F IBDM, CNRS Case 907 *F Parc Scientifique de Luminy *F 13288 Marseille Cedex 9 *F France *F Tel:(0)4 91 26 96 03 *F Fax: (0)4 91 82 06 82 # *U FBrf0099368 *a Lindsley *b D. *t 1997.10.13 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Dan Lindsley, University of California, San Diego *F To: Bloomington Drosophila Stock Center *F Subject: Aberrations in the Mid-America Stock List *F Date: 12 October 1997 *F *F Background: We asked Dan if he could identify any of the aberrations from the Mid-America stock list that weren't in FlyBase *F or provide any additional information on some that were in FlyBase. Information he provided that is relevant to FlyBase follows. *F *F Information communicated: *F *F 1. Dp(1;YL)sc[S1]3 *F *F "I found this Dp. It is an independent occurrence, but there's not much to recommend keeping it. As far as I knew it was long *F gone." *F *F 2. Dp(1;YL)y[+] T0 *F *F "I almost couldn't dredge up the identity of T0. It is the result of a recombinant between YS and the distal heterochromatin of *F In(1)sc[8]. It is from my thesis in Genetics 40 page 28." *F *F 3. Dp(1;YS)sc[V1] *F *F "Dp(1;YS)sc[V1] is an interesting chromosome. It was derived from an exchange between proximal YS and the proximal Xhet *F of the pericentric inversion, In(1LR)sc[V1] and therefore has y[+] and ac[+] on the other side of the centromere from YS." *F *F 4. Dp(1;YL)y[+]YL, also known as FR2 and y[+]YL. *F *F "The reason that Novitski called this a fragment is that it seems to be a derivative of only the centromere end of C(1;Y)1. The *F fact that it is bb[+] indicates that the exchange appending YL to In(1)EN involved the short arm of Y distal to bb[+], since *F In(1)EN carries bb at both ends." *F *F 5. YSX[.]P0 *F *F "Remarkably, this is another derivative from my thesis. It resulted from an exchange between YS and the distal heterochromatin *F of In(1)sc[8L]EN[R]. Genetics 40, page 28." *F # *U FBrf0099369 *a Lindsley *b D. *t 1997.12.1 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Dan Lindsley, University of California, San Diego *F To: Bloomington Drosophila Stock Center *F Subject: Aberrations in the Mid-America Stock List *F Date: 1 December 1997 *F *F Background: We asked Dan if he could identify any of the aberrations from the Mid-America stock list that weren't in FlyBase *F or provide any additional information on some that were in FlyBase. Information he provided that is relevant to FlyBase follows. *F *F Information communicated: *F *F 1. C(1;Y)112-17 & C(1;Y)129-11 *F "C(1;Y)112-17 & 129-11 are XYL.YS with the X in normal sequence: Like C(1;Y)6 I suppose." *F *F 2. C(1;Y)110-8 & C(1;Y)115-9 *F "C(1;Y)110-8 & 115-9 are XYS.YL with the X in normal sequence: Like C(1;Y)8?" *F *F 3. Dp(1;1)L *F "These are derivatives of a normal X with heterochromatin appended to the tip without the loss of any vital loci. One carries B[S] *F from the B[S]Y, one carries y[31d] from In(1)sc[8], and one carries y[+] from y[+]Y. They were made by me and used in the *F construction of Dp(1;1)B[S]TAG, which apparently no longer exists." *F *F 4. Dp(1;3)sc[J4] *F "Dp(1;3)sc[J4] is a useful chromosome; it carries y[+] appended to the end of 3L without the loss of any vital third chromosome *F loci. Useful in somatic mosaics as an alternative to mwh[+]." *F *F 5. Dp(1;Y)B[S]v[+]y[+] *F "Dp(1;Y)B[S]v[+]y[+] is the only Y carrying a normal allele of v." *F # *U FBrf0099485 *a Roote *b J. *t 1997.11.26 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Nov 26 14:08:41 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Wed, 26 Nov 1997 14:08:41 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Wed, 26 Nov 1997 14:15:36 +0000 *F To: Aubrey de Grey *F From: John Roote *F Subject: new kuz allele *F Content-Length: 236 *F I have a new osp allele which for want of better I've called ospPW. *F Local hop from 35Bck08808 - the new stock contains both k08808 and *F ospPW. *F John # *U FBrf0099486 *a Roote *b J. *t 1997.12.2 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Dec 02 19:26:41 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 2 Dec 1997 19:26:41 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 2 Dec 1997 19:33:41 +0000 *F To: Aubrey de Grey *F From: John Roote *F Subject: Re: l(2)35Fh *F Content-Length: 491 *F >Hi John, I have just discovered a gene 'l(2)35Fh' in FlyBase which has *F >only one ref, namely a short PNAS review by Spradling of the P screen. *F >No alleles, nothing. Have you heard of it? *F > *F >Cheers, Aubrey *F We use the name 35Fh for a gene inferred from the lethality of el18/RN2 - *F should only overlap for twe but twe is not a lethal. *F John # *U FBrf0099492 *a Russell *b R. *t 1997.11.25 *T personal communication to FlyBase *u *F Date: Tue, 25 Nov 1997 12:09:31 +1000 *F To: m.ashburner@gen.cam.ac.uk *F From: robynr@ento.csiro.au (Robyn Russell) *F Subject: esterase queries *F Content-Length: 30386 *F Dear Dr Ashburner *F John Oakeshott passed your esterase queries on to me. *F We note that FlyBase lists the cytological site of alphaE7 as 84D3 - we *F would like to change that to 84D3-10 (see Russell et al. 1996, Insect *F Biochem. Molec. Biol. 26: 235). *F >FlyBase is curating your paper on the a-esterase cluster. Can any of these *F >be tied to Ali, Est9, Mce yet? If not, we will have to create new genes *F >for the sequenced ones. *F The work of Campbell et al. (Biochemical genetics, 35:17-40, 1997, and *F Insect Biochemistry and Molecular Biology, in press) have shown that ALI *F and MCE in Lucilia cuprina are encoded by alleles of the LcaE7 gene (ie *F esterase E3 = ALI = MCE), the orthologue of DmaE7. Robin et al. (in prep) *F have confirmed by in vitro expression that DmaE7 encodes EST23 in D. *F melanogaster, which was shown by Spackman et al. (Biochemical Genetics *F 32:39, 1994) to be the likely homologue of esterase E3 in Lucilia. The *F expressed product of DmaE7 also had ali-esterase and MCE activities. As *F yet we have not identified the gene encoding EST9. *F Comments on the status of the following genes: *F Est20 (Campbell et al., 1992, Insect Biochem Molec. Biol. 22:665) *F Est20 in D. melanogaster is most probably the orthologue of the p-esterase *F in D. virilis. The latter may be a minor component of JHE activity *F (Klebodarova et al.,1996, Insect Biochem. Molec. Biol. 26:829) but EST20 is *F not JHE. *F Est9 of Loukas (1981, DIS 66:85) *F This gene maps to chromosome 2R and is the orthologue of Est9 in D. *F subobscura. The esterase is localised to the adult head and is detected *F only when 1-leucyl-b-naphthylamide is used as a substrate together with *F a-naphthyl acetate. As far as we know the gene has not been cloned, nor *F was it identified by Healy et al. (1991, Biochemical Genetics 29:365). The *F nomenclature is unfortunate but we refer to it as 'Est9 of Loukas'. *F EstA (Mizianty and Case, 1971, J. Hered, 62: 345) *F This gene is most probably Est18 of Healy et al. (1991, Biochemical *F Genetics 29: 365). As far we know Est18 has been neither mapped nor *F cloned. *F EstF (Chang and Lin, 1990, Bull. Inst. Zool. Acad. Sinica, Taiwan 29: 81 *F and 1995, Zool. Studies 34: 47) *F This gene encodes an esterase dimer in D. albomicans that is polymorphic in *F natural populations. We have no idea where it maps or which of the D. *F melanogaster esterases it might be orthologous to. *F Regards, Robyn Russell *F From ma11@gen.cam.ac.uk Tue Nov 25 08:57:25 1997 *F To: m.ashburner@gen.cam.ac.uk, robynr@ento.csiro.au *F Subject: Re: esterase queries *F Date: Tue, 25 Nov 1997 09:04:33 +0000 *F Content-Length: 401 *F Dear Robyn Russell *F Thank you very much for your detailed reply. Some questions: *F 1. You say that alphaE7 is 84D3-10; fine, but the others in this cluster *F are at 84D3, is that OK ? *F 2. I have merged the following three FlyBase records into one gene (called *F alphaE7, although the symbol Ali has priority it seems better to keep *F this more 'systematic' name and symbol): Ali, Est-23, Mce. *F Many thanks *F Date: Wed, 26 Nov 1997 09:55:15 +1000 *F To: Michael Ashburner (Genetics) *F From: robynr@ento.csiro.au (Robyn Russell) *F Subject: Re: esterase queries *F Dear Dr Ashburner *F I will put my replies under your queries: *F > *F >1. You say that alphaE7 is 84D3-10; fine, but the others in this cluster *F >are at 84D3, is that OK ? *F Not exactly. The original YAC clone containing the cluster maps to 84D3-10 *F by in situ hybridisation. We do not know exactly where in that 300kb the *F cluster is situated, so the best position we have for the 10 genes we have *F identified from that YAC clone is 84D3-10. *F > *F > *F >2. I have merged the following three FlyBase records into one gene (called *F >alphaE7, although the symbol Ali has priority it seems better to keep *F >this more 'systematic' name and symbol): Ali, Est-23, Mce. *F Agreed. *F > *F >Thanks and regards, Robyn *F ______________________________________ *F Robyn Russell, Principal Research Scientist *F Biotechnology Program, CSIRO Division of Entomology *F GPO Box 1700 CANBERRA ACT 2601 AUSTRALIA *F Tel: +61 6 246 4160 Fax: +61 246 4173 *F e-mail: robynr@ento.csiro.au *F ___________________________________ # *U FBrf0099633 *a Zak *b N. *c M. *d Crosby *t 1997.1.12 *T personal communication to FlyBase *u *F From crosby@morgan.harvard.edu Mon Dec 01 19:03:57 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 1 Dec 1997 19:03:57 +0000 *F Date: Mon, 01 Dec 1997 14:09:37 -0500 *F From: crosby@morgan.harvard.edu (Madeline Crosby) *F Subject: moira and 89B helicase *F To: flybase-updates@morgan.harvard.edu *F Cc: crosby@morgan.harvard.edu, zakn@gene.md.huji.ac.il *F Content-transfer-encoding: 7BIT *F X-Sun-Charset: US-ASCII *F Content-Length: 738 *F FlyBase, *F We would like to submit the following correction as a personal *F communication to FlyBase: *F We have determined that the identification of 89B helicase as the *F product of the moira gene was in error (FBrf0090578). Please *F remove all references to 89B helicase from the moira record; *F 89B helicase is a new, previously unidentified gene. *F The GenBank accession number cited in the moira record is that *F of 89B helicase; it should also be deleted from the moira record *F and moved to the 89B helicase record. *F Sincerely, *F Naomi Zak *F Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, *F Hebrew University-Hadassah Medical School *F Madeline Crosby *F Department of Molecular and Cellular Biology, Harvard University # *U FBrf0099648 *a Wasserman *b S. *t 19?? *T personal communication to FlyBase *u *F Unarchived. # *U FBrf0099662 *a Roote *b J. *t 1997.12.2 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Dec 02 10:35:31 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 2 Dec 1997 10:35:31 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 2 Dec 1997 10:42:22 +0000 *F To: Aubrey de Grey *F From: John Roote *F Subject: fs(2)lto1 *F Content-Length: 113 *F Aubrey, *F fs(2)lto1 = PN48 (35E6--36A7) is fertile with Df(2L)A48, r10 and chif64 *F (covering 35B2.3;36D). *F John # *U FBrf0099663 *a Bloomington Drosophila Stock Center *b ?. *t 1997.12.5 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Assorted alleles and aberrations not in FlyBase and some corrections *F Date: 5 December 1997 *F *F Information communicated: *F *F 1. E(gammaTub37C[1]) *F *F Allele symbol E(gammaTub37C[1])[1] *F Map location chromosome 2 *F Phenotype enhances gammaTub37C[1] *F Discoverer Ted Wright *F *F 2. l(2)N7-3[1] *F l(2)N7-6[1] *F l(2)N7-8[1] *F *F To Bowling Green from Daniel Kalderone; I have no further info on any of these. *F *F 3. sm[BS] *F Allele symbol sn[BS] *F Discoverer Bruce Sheldon *F Mutagen spontaneous *F Phenotype initially recessive female sterile, fertility improved over many years in stock *F *F 4. l(4)K-2[1] *F Nothing else known. *F *F 5. Please correct: *F Pgi[nNc80] --> Pgi[nNC80] *F *F 6. Please change the 'unspecified' allele designations for these gene to the following: *F l(3)72Ab[I24] (that's an uppercase letter I) *F l(3)72Ac[I10] *F l(3)72Ad[I25] *F l(3)72CDa[J12] *F l(3)72CDb[M3] *F l(3)72CDc[K5] *F l(3)72CDd[L2] *F l(3)72CDe[I1] *F l(3)72CDf[I6] *F *F 7. In(1)sc[8L]R(1)2[R] *F Aberration symbol In(1)sc[8L]R(1)2[R] *F Discoverer Dan Lindsley *F *F 8. R(1)5 *F progenitor: In(1)w[m4] *F Aberration symbol R(1;YL) *F Progenitors In(1)EN & R(YL) *F *F 9. T(3;4)Dubinin *F Aberration symbol T(3;4)Dubinin *F Breakpoints presumably 80-81;h59-102F8 (based on statement in Mid-America stock documentation 'separated arms of 3') # *U FBrf0099907 *a Roote *b J. *t 1997.12.23 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Tue Dec 23 11:48:56 1997 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 23 Dec 1997 11:48:56 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 23 Dec 1997 11:56:08 +0000 *F To: Michael Ashburner , *F Aubrey de Grey *F From: John Roote *F Subject: k08310 *F Content-Length: 63 *F k08310 is a twe allele (male sterile but female fertile). *F J # *U FBrf0099908 *a Levin *b L. *t 1997.12.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Dec 22 14:54:09 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 22 Dec 1997 14:54:09 +0000 *F To: llevin@Med.cornell.edu *F Subject: Helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F From: Rachel Drysdale (Genetics) *F Date: Mon, 22 Dec 1997 15:01:28 +0000 *F Content-Length: 1765 *F Hi Lonny, *F I'm just going through the 1997 abstract book for the CSH neurobiology *F meeting and have a question for you. On page 78 you describe two adenyl *F cyclase isoforms, 'DAC3' and 'DAC9'. FlyBase already has a gene for DAC9 *F (valid symbol Ac35C) but DAC3 is new to us. We have entries for *F three genes that may correspond to DAC3: *F \*a Ac34A *F \*e Adenylyl cyclase 34A *F \*z FBgn0004853 *F \*b 2-47 *F \*c 34A *F \*x FBrf0078595 == ew1786 == Han and Davis, 1995, A. Conf. Dros. Res. 36: 91B *F \*x FBrf0055565 == ewcell68.479f.mgUCLAKX == Levin et al., 1992, Cell 68: 479--489 *F \*F adenylate cyclase == EC 4.6.1.1 *F \*a Ac62D *F \*e Adenylyl cyclase 62D *F \*z FBgn0004851 *F \*b 3-1.5 *F \*c 62D *F \*x FBrf0078595 == ew1786 == Han and Davis, 1995, A. Conf. Dros. Res. 36: 91B *F \*x FBrf0055565 == ewcell68.479f.mgUCLAKX == Levin et al., 1992, Cell 68: 479--489 *F \*F adenylate cyclase == EC 4.6.1.1 *F \*a Ac76E *F \*e Adenylyl cyclase 76E *F \*z FBgn0004852 *F \*b 3-46 *F \*c 76E *F \*x FBrf0078595 == ew1786 == Han and Davis, 1995, A. Conf. Dros. Res. 36: 91B *F \*x FBrf0055565 == ewcell68.479f.mgUCLAKX == Levin et al., 1992, Cell 68: 479--489 *F \*F adenylate cyclase == EC 4.6.1.1 *F Do any of these encode DAC3? If not we will keep a new gene entry, Ac3 *F (with a synonym DAC3, we do not prefix Drosophila genes with a D), but if *F so we will make the DAC3 entry under the appropriate gene. *F With best wishes, *F Rachel. *F \--------------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \--------------------------------------------------------------------- *F From llevin@mail.med.cornell.edu Tue Dec 23 17:01:00 1997 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 23 Dec 1997 17:01:00 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 23 Dec 1997 12:09:09 -0500 *F To: Rachel Drysdale (Genetics) *F From: 'Lonny R. Levin' *F Subject: Re: Helping FlyBase *F Content-Length: 2080 *F Dear Rachel, *F DAC3 is distinct from the other Adenylyl cyclase genes already listed in *F Flybase. *F It has not yet been published. We have deposited its sequence into GENBANK, *F but it has not been released yet (we are awaiting its publication). *F For the sake of completeness, it maps to the interval 39E5-F3. *F While you are updating the AC entries in Flybase, would you please add the *F synonym 'DAC2' to the entry for Ac76E....we have completed cloning this *F cyclase and it is most closely related to mammalian AC2. As with the others *F I have mentioned, we are not quite ready to publish the cloning of this AC, *F but it would be nice for Flybase to be as up-to-date as we are. *F Thanks again. *F lrl *F _____________________________________________ _______ *F Lonny R. Levin *F Department of Pharmacology *F Cornell University Medical College *F 1300 York Avenue Rm. E-505 *F New York, NY 10021 *F Office: 212-746-6752 *F Lab: 212-746-6750 *F FAX: 212-746-8835 *F llevin@mail.med.cornell.edu *F http://www-users.med.cornell.edu/~llevin/default.html *F http://www-users.med.cornell.edu/~llevin/GPIP/PharmProgram.html *F _____________________________________________ _______ # *U FBrf0099909 *a Beaton *b A. *t 1997.12.27 *T personal communication to FlyBase *u *F >From abeaton@uclink4.berkeley.edu Sat Dec 27 00:26:42 1997 *F I had been suspecting that the combination of PxKr and Cy would make the *F wings unscorable and in fact I was right. I tested a few folks on the *F discrimination and.. well failure. So I rebalanced the In PxKr over a CyO *F we have with activated ras that makes rough eyes and rebalanced the Ps from *F the region over the T(2:3)SM6;TM6B, (which I can't find as an ab), so I *F could score the Tb. Anyway there were some conflicting data so I *F suppressed what I had had and entered the new. *F So, now In(2R)bwVDe2LPxKR *F Deletes or disrupts *F l(2)k06908 59E1-2 this one has a Bellen name chrw *F Dcp-1 59F2-3 *F l(2)02535 59F3-4 *F l(2)03041 59F3-4 *F l(2)k13108 60A3-4 *F l(2)k13214 60A3-4 *F l(2)02970 60A5-9 *F l(2)00628 60A8-11 *F l(2)k09025 60A8-11 *F Phm 60B1-2 *F bsl(2)03267 60C6-8 *F l(2)k08003 60D1-2 *F and complements these verifieds *F l(2)06496 59C1-4 *F l(2)06369 59F1-2 *F l(2)09049 59F1-2 apt *F l(2)05006 60B1-2 *F l(2)k04201 60B1-2 *F l(2)k04405 60B1-2 66E1-2 *F l(2)k13705 60B11-13 *F l(2)k12101 60B12-13 *F l(2)01296 60B4-5 *F l(2)k05633 60B4-5 *F l(2)k05318 60C1-2 *F l(2)k16102 60D5-6 *F l(2)01092 60E1-2 Dll *F l(2)10481 60E11-12 *F l(2)01155 60F1-3 gsb-d *F \---- End Included Message ----- # *U FBrf0099910 *a Beaton *b A. *t 1998.1.8 *T personal communication to FlyBase *u *F From abeaton@uclink4.berkeley.edu Thu Jan 08 17:47:17 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 8 Jan 1998 17:47:17 +0000 *F Date: Thu, 8 Jan 1998 09:47:12 -0800 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F X-mailer: Eudora Pro 2.1.4 *F To: Rachel Drysdale (Genetics) *F From: abeaton@uclink4.berkeley.edu (Amy Beaton) *F Subject: Re: In(2R)bwVDe2LPxKR complementation data *F Content-Length: 3055 *F . *F . *F I checked with Todd and he had a look at the slide and revised the *F position of 01862 to 59E3-4. So I think you could call Dcp-1 59E3-4. *F . *F . *F Amy # *U FBrf0099937 *a Alatortsev *b V.E. *t 1998.1.31 *T personal communication to FlyBase *u *F From Alatortsev@img.ras.ru Sat Jan 31 11:09:22 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Sat, 31 Jan 1998 11:09:22 +0000 *F From: Alatortsev *F To: ''Flybase'' *F Subject: correct description of the Vinc gene *F Date: Sat, 31 Jan 1998 14:16:49 +-300 *F MIME-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-Transfer-Encoding: quoted-printable *F Content-Length: 984 *F Dear Colleagues, *F We find that Drosophila has a single copy Vinculin (Vinc) gene, which is situated in the 2E region of the X chromosome *F ( Alatortsev V.E., Kramerova I.A., Frolov M.V., Lavrov S.A., *F Westphal E. D. (1997) Vinculin gene is non-essential in Drosophila *F melanogaster. FEBS Letters, v. 413, pp. 197-201.). *F I'd recommend to move off Flybase the Vin (84C8) gene report as *F uncorrect. This report is based only on data from abstract of the 32th *F Dros. Res. Conf., which did not supported by journal publication since *F 1991. All the data of Drs. Alatortsev and Kramerova mentioned in this *F report are related to the Vinc gene from the 2E region and must be moved *F to the Vinc gene report (gene symbol Vin2EF must be replaced by Vinc). *F Sincerely, *F Dr. Vladimir E. Alatortsev *F Institute of Molecular Genetics *F Russian Academy of Sciences *F Kurchatov Sq. 46 *F Moscow, RUSSIA *F Phone: 7 095 1965901 *F FAX: 7 095 1960221=20 *F Email: Alatortsev@img.ras.ru # *U FBrf0099938 *a Alexandrova *b M. *t 1998.1.26 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Jan 05 15:54:01 1998 *F To: alexandr@nusun.jinr.dubna.su *F Dear Rita, *F Happy New Year! *F Thank you very much for your wonderfully useful reply. I am sorry it has *F taken me so long to get back to you but I was on holiday until today. *F After going through your message I have only two remaining questions: *F >2050 In(2L)C163, l(2)br48[AM4]=l(2)35Eb, l(2)CA7/CyO *F Your listing suggests two mutant alleles, but we so far have no record of *F l(2)br48[AM4], l(2)35Eb or l(2)CA7. Do you know whether these mutations *F are caused by the inversion breaks, or are they just incidental lethals. The *F cytological data we have for In(2L)C163.41 suggests that 'l(2)35Eb[AM4]' *F might be caused by the proximal inversion break. What about 'l(2)CA7'? *F > Cy[329] = Cy[2004]. *F > See references for it in our stock 2004 (DIS 80) and *F > DIS (1987) 65:107. *F I am afraid I could not find what I needed in either of these places. So *F is this a new allele of Cy or just the allele represented on the In(2LR)Cy *F chromosome? *F Thank you for these last bits of information. *F with best wishes, *F Rachel. *F ---------------------------------------------------------------------- *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F ---------------------------------------------------------------------- *F From alexandr@nusun2.jinr.dubna.su Mon Jan 26 17:34:57 1998 *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F After some delay in answering your message of 5 Jan, *F I can say concerning two points put by you as follows: *F 2050 Here there is a typographical error. It will be *F correct: In(2L)C163.41. l(2)br48[AM4] = l(2)35Eb is, indeed, *F caused by the proximal inversion break (see DIS-64 (1986) *F 50). About l(2)CA7 I can say nothing. We received this chromosome *F from Dr. M.Ashburner in March, 1987 without the description of *F the l(2)CA7. If you will have some information about it let me *F please know. *F Cy[329] and Cy[2004] are the numbers of the same *F In(2LR)Cy chromosome - balancer with the appropriate *F markers (see our stock 2004 in DIS-80 (1997) 117). *F In fact, this is a short-cut name of this balancer. *F With best wishes, *F Rita. # *U FBrf0099981 *a Burtis *b K. *t 1998.1.13 *T personal communication to FlyBase *u *F From kcburtis@ucdavis.edu Tue Jan 13 22:08:04 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 13 Jan 1998 22:08:04 +0000 *F Date: Tue, 13 Jan 1998 14:16:23 -0800 *F From: kcburtis@ucdavis.edu (Ken Burtis) *F Subject: mus309 / Ypf1b / IRBP / Horka *F X-Sender: fzburtis@mailbox.ucdavis.edu *F To: flybase-updates@morgan.harvard.edu *F MIME-version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-transfer-encoding: 7BIT *F Content-Length: 2306 *F Dear Flybase, *F I wish to draw your attention to an error in the entry for the gene *F 'Ypf1b', as well as the need for additional links in that entry. The *F present entry as I downloaded it is at the bottom of this message. *F >From the sequence of Ypf1b, as well as its identity, it is clearly the same *F gene as the P element inverted repeat binding protein (IRBP), which Don Rio *F has clearly shown to be the same as mus309. All are the Drosophila homolog *F of the smaller 70 kd subunit of the Ku heterodimer. *F The error in this entry is the cytological location, which should be *F 87B1--87B2, the location of mus309, and not 34C1--34C7 as reported in *F FBrf0068542 (Jacoby & Wensink). Presumably their in situ hybridization gave *F erroneous results. *F A link should also be added to the entry for Ypf1b indicating its identity *F with mus309 and IRBP (and presumably to the entries for these genes as well *F indicating the connection to Ypf1b). *F Sincerely yours, *F Ken Burtis *F From rd120@gen.cam.ac.uk Wed Jan 14 10:29:25 1998 *F Hi Ken, *F Thank you for all this information. Your mail will be curated as a personal *F communication to FlyBase, and the changes it precipitates in the master *F file will become visible when that version of the master file reaches the *F public server, probably in a few weeks. *F The entries for the mus309 and Ypf1b genes will be merged, with mus309 *F being kept as the valid symbol, since the references for mus309 predate *F those for Ypf1b. We have already merged IRBP with mus309 so that is taken *F care of. *F I will see to it that the report of the '34C1--34C7' is sorted out. *F thanks for getting in touch, *F with best wishes, *F Rachel. # *U FBrf0100029 *a Frasch *b M. *t 1998.1.16 *T personal communication to FlyBase *u *F From frasch@msvax.mssm.edu Fri Jan 16 21:56:09 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 16 Jan 1998 21:56:09 +0000 *F Date: Fri, 16 Jan 1998 16:59:38 -0500 *F From: Manfred Frasch *F Subject: l(3)03852 *F To: flybase-updates@morgan.harvard.edu *F MIME-version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-transfer-encoding: 7BIT *F Content-Length: 396 *F We find that the above P-mutation is allelic to mod(mdg4), since it fails *F to complement our EMS allele mod(mdg4)20. The STS Dm0283 that was *F obtained from this insertion by the genome project corresponds to genomic *F sequences (intron sequences) ~1kb downstream of the presumed transcription *F start site of mod(mdg4). *F Please contact me if you have further questions. *F Sincerely, *F Manfred Frasch # *U FBrf0100224 *a Roote *b J. *t 1998.1.20 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Jan 20 12:14:45 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 20 Jan 1998 12:14:45 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Date: Tue, 20 Jan 1998 12:22:14 +0000 *F To: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: Re: Moscow stocks *F Content-Length: 256 *F Hi Rach, *F l(2)CA2 was invented to explain why 35BcHG1 was lethal with laceAM1 *F l(2)CA5 ditto laceHG34 and Su(H)LT3 *F l(2)CA8 ditto 34FaHG15 and Df(2L)PA4 *F We didn't do anything with them, so no idea where they map (other than *F which chromosome!). *F BFN, *F John # *U FBrf0100236 *a Schaefer *b M. *t 1998.1.22 *T personal communication to FlyBase *u *F From mschaef2@gwdg.de Thu Jan 22 08:00:12 1998 *F To: flybase-updates@morgan.harvard.edu *F Dear colleagues, *F Under the heading of synonyms I found also Mstprox, which I think is *F inappropriate. Mstprox refers to a different gene immediately adjacent to *F Mst84Da which encodes an interleukin-1 receptor-type protein and to my *F knowledge is not a male specific transcript (mst). The genes may overlap *F with their 3'ends (UTR sequences, see discussion in reference given *F above), since they are located on opposite strands and RNA size etc. is *F not described so far. *F Best regards, Mireille Schaefer. *F \-- *F Dr. Mireille Schaefer *F Abt. Molekulare Entwicklungsgenetik *F Humboldtallee 34A *F D-37073 GOETTINGEN, Germany *F e-mail: mschaef2@gwdg.de; phone/fax +49-551-392867 # *U FBrf0100265 *a Tomita *b K. *t 1998.2.2 *T personal communication to FlyBase *u *F >From tomita@kwl.t.u-tokyo.ac.jp Mon Feb 02 11:14:11 1998 *F Subject: Re: Help FlyBase *F Dear Dr Tomita *F I want to curate the following sequence records for FlyBase. Could you *F tell me which tRNA sequences each is ? Thankyou. *F Michael Ashburner *F OK. *F ACCESSION AB009831 for Drosophila melanogaster mitochondiral tRNAAsn *F ACCESSION AB009832 for Drosophila melanogaster mitochondiral tRNAIle *F ACCESSION AB009833 for Drosophila melanogaster mitochondiral tRNAMet1 *F ACCESSION AB009834 for Drosophila melanogaster mitochondiral tRNAMet2 *F ACCESSION AB009835 for Drosophila melanogaster mitochondiral tRNALys *F ACCESSION AB009836 for Drosophila melanogaster mitochondiral tRNASerGCU *F ACCESSION AB009837 for Drosophila melanogaster mitochondiral tRNATrp *F If you have some questions, do not hesitate to ask me again. *F Kozo Tomita *F Department of Chemistry and Biotechnology *F Faculty of Engineering *F University of Tokyo *F Bunkyo-ku, Tokyo 113, JAPAN *F Tel and Fax; +81 (0)3 5800-6950 *F E. mail; tomita@kwl.t.u-tokyo.ac.jp # *U FBrf0100323 *a Bloomington Drosophila Stock Center *b ?. *t 1998.1.13 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: synonyms for l(4) complementation groups and new alleles *F Date: 13 January 1998 *F *F Information communicated: *F *F 1. Please add the following synonyms: *F *F l(4)18[s] as a synonym for l(4)102ABe[1] *F l(4)7[g] as a synonym for l(4)102CDf[1] *F l(4)27[B] as a synonym for l(4)102CDm[1] *F l(4)28[C] as a synonym for l(4)102CDn[1] *F l(4)30[E] as a synonym for l(4)102CDo[1] *F l(4)31[F] as a synonym for l(4)102CDp[1] *F l(4)32[G] as a synonym for l(4)102CDq[1] *F *F The case of the letter superscripts is how these appear in the information we got from Mid-America - perhaps typos. *F *F 2. Please create records for the following alleles: *F *F l(4)102CDj[p] note that this might be the same as l(4)102CDj[1] (this was a Mid-America stock, now at Bloomington) *F l(4)102CDl[w] note that this might be the same as l(4)102CDl[1] (this was a Mid-America stock, now at Bloomington) *F l(4)102CDt[K] note that this might be the same as l(4)102CDt[1] (this was a Mid-America stock, now at Bloomington) *F # *U FBrf0100324 *a Bloomington Drosophila Stock Center *b ?. *t 1998.1.9 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Alleles and aberrations not in FlyBase and some corrections *F Date: 9 January 1998 *F *F Information communicated: *F *F 1. Please create records for the following alleles: *F *F brm[I21] to Mid-America from Daniel Kalderon *F Est-C[n] null allele, to Mid-America from Rollin Richmond *F *F 2. Please create records for the following gene and allele: *F *F l(3)7m62[1] to Bloomington from Adelaide Carpenter *F *F 3. Please create records for the following aberrations: *F *F C(1;1;YS)RM[.]YS *F probably YSIn(1)EN[.]In(1)ENYS, from attachment of C(1;Y)1, per Dan Lindsley *F C(1;1;YL)RA[.]YL *F one X wild-type, one In(1)sc[8L]EN[R], y[1], and YL carries y[+], centromere presumably from C(1;Y)1, per Dan L. *F F(YS)1 *F YS on an X chromosome centromere, bb[+], per Dan Lindsley *F C(1;Y)6 *F In(1)EN2[.]YS *F Ts(1Rt;4Rt)w[258-21] *F separable component of FBab0006552 *F R(1;YL)19-20d *F to Mid-America from J. Lucchesi *F R(Y)F(9) and R(Y)F(H2) *F presumably two of the 7 mentioned in the R(1)F record (FBab0005905) *F *F 4. Remove aberration Dp(1;Y)y[3M]sc[S1]. This is really Dp(1;YL)sc[S1], y[3M] *F Since this is a genotype there is nothing to keep the aberration symbol as a syn for, but it might help clarify things if you changed *F 'Y[L][.]sc[S1]' in the Miscellaneous Info field of the y[3M] record to Dp(1;YL)sc[S1]. # *U FBrf0100404 *a Matthews *b K. *t 1998.2.5 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: map data for GAL4 enhancer traps *F Date: 5 February 1998 *F *F Information communicated: *F The following enhancer-trap insertions described in FBrf0095642 have been mapped at the Bloomington Stock Center to the chromosomes indicated. *F *F Insertion Chromosome *F P{GawB}185Y 1 *F P{GawB}c323a 1 *F P{GawB}179Y 1 *F P{GawB}c381 4 *F P{GawB}c701b 1 *F P{GawB}c338 3 *F P{GawB}c698a 1 *F P{GawB}c368 2 *F *F # *U FBrf0100405 *a Schneider *b L. *t 1998.3.9 *T personal communication to FlyBase *u *F From leschnei@zoo.uvm.edu Mon Mar 9 15:50:57 1998 *F X-Sender: leschnei@pop.uvm.edu *F Mime-Version: 1.0 *F Date: Mon, 9 Mar 1998 15:53:22 -0400 *F To: bmatthew@morgan.harvard.edu (Beverley Matthews) *F From: leschnei@zoo.uvm.edu (Lynne Schneider) *F Subject: Re: Gprk2 map information request *F *F Berverly, *F Here is the information on the Gprk2 gene. *F The exon sizes are as follows: *F Exon 1: 688 bp *F Exon 2: 48 *F Exon 3: 430 *F Exon 4: 96 *F Exon 5: 121 *F Exon 6: 607 *F Exon 7: 106 *F Exon 8: 191 *F Exon 9: 249 *F Exon 10: 158 *F Exon 11: 210 *F Exon 12: 265 *F Exon 13: 382 *F *F I have information for introns 4-12. Here are the sizes: *F Intron 4: 59 bp *F Intron 5: 64 *F Intron 6: 138 *F Intron 7: 74 *F Intron 8: 442 *F Intron 9: 72 *F Intron 10: 58 *F Intron 11: 94 *F Intron 12: 61 *F *F The gap between exons 1 and 3 is very large (at least 15 kb, I estimate), *F and I have not been able to map exon 2 within that gap. Intron 3 is *F probably around 1 kb (again an estimate). *F *F >Dear Dr. Schneider, *F > *F >I am a molecular curator for FlyBase. I would like to enter map *F >information about Gprk2 into FlyBase but it would help to have some *F >more specific information than was provided in your Development *F >124:2591 paper. The exon sizes can be determined from the sequence in *F >the paper but as far as I can tell, the intron sizes are not provided. *F >It would help enormously if you could provide me with the exact exon *F >and intron sizes (insofar as you know them) so I can create an accurate *F >map for FlyBase. The information will be treated as a personal *F >communication from you to FlyBase. *F > *F >Thanks for your help. *F > *F >Beverley Matthews *F >for FlyBase *F >bmatthew@morgan.harvard.edu *F *F Lynne Schneider *F University of Vermont *F Department of Biology *F 311 Marsh Life Sciences *F Burlington VT 05405 *F *F Errata follows: *F *F From leschnei@zoo.uvm.edu Wed Mar 11 12:01:52 1998 *F X-Sender: leschnei@pop.uvm.edu *F Mime-Version: 1.0 *F Date: Wed, 11 Mar 1998 12:04:19 -0400 *F To: bmatthew@morgan.harvard.edu (Beverley Matthews) *F From: leschnei@zoo.uvm.edu (Lynne Schneider) *F Subject: Re: Gprk2 map information request *F *F Dear Beverley, *F Sorry, I was looking at an old version of the sequence. The following *F numbers are correct: *F *F exon 1 692 *F exon 6 606 *F exon 8 190 *F exon 12 264 *F exon 13 362 *F *F Exon 1 is an update to the paper. The other numbers are as I sent in my *F previous message. Sorry about the mix-up. *F Lynne *F *F >Dear Dr. Schneider, *F > *F >Sorry to bother you again but I've just noticed that some of the exon *F >sizes you gave me don't match the information I derived from Figure 3. *F >Are these real updates to the paper? Thanks. *F > *F > Fig. 3 personal communication *F > *F >exon 1 690 688 *F >exon 6 606 607 *F >exon 8 190 191 *F >exon 12 264 265 *F >exon 13 362 (to poly A) 382 *F > *F > *F > *F >Beverley *F *F Lynne Schneider *F University of Vermont *F Department of Biology *F 311 Marsh Life Sciences *F Burlington VT 05405 *F *F # *U FBrf0100486 *a Roote *b J. *t 1993.5.27 *T personal communication to FlyBase *u *F [original text unavailable] *F T(2;3)H47 is osp[-] *F T(2;3)H50 is sna[-] *F T(2;3)H67 is ck[-] *F T(2;3)H68 is wb[-] # *U FBrf0100702 *a Eberl *b D. *t 1997.2.16 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Thu Feb 12 15:10:19 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Thu, 12 Feb 1998 15:10:19 +0000 *F To: eberl@rascal.med.harvard.edu *F Subject: Help FlyBase - auditory mutants *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Thu, 12 Feb 1998 15:18:13 +0000 *F Content-Length: 1293 *F Hi, *F I am curating a paper of yours for FlyBase: *F FBrf0100015 == Eberl et al., 1997, Proc. Natl. Acad. Sci. USA. 94(26): *F 14837--14842 *F in which you describe many auditory mutants. *F You provide designations for the mutants: *F 5G23, 5M8, 5M38, 5N15, 5N18, 5N29, 5D10, 5E8, 5F3, 5M7, 5N17, 5N30, 5P1 and *F 5L3. *F Apart from 5G10 (which is pir) none of these are in FlyBase. *F I would like to generate a gene symbol for these mutants so that they form *F a gene series. Present examples are 'ms' for male sterile, 'l' for lethal. *F Would you be opposed to the above symbols being prefixed with an 'ad' for *F auditory defective (or another prefix of your choice)? *F An example would be: *F ad5G23 *F and where we know the chromosome location *F ad(2)5M8 *F Do you like this idea or would prefer the genes to be named simply after *F the line designation? *F Thanks for your help, *F Regards, *F Eleanor Whitfield *F FlyBase *F \-------------------------------------------------------------- *F Eleanor Whitfield. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email : eleanor@gen.cam.ac.uk *F Cambridge, CB2 3EH, Phone : 44 (0)1223-333963 *F UK. FAX : 44 (0)1223-333992 *F \-------------------------------------------------------------- *F From eberl@rascal.med.harvard.edu Fri Feb 13 21:26:27 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 13 Feb 1998 21:26:27 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Transfer-Encoding: binary *F Date: Fri, 13 Feb 1998 16:35:40 -0400 *F To: Eleanor Whitfield (Genetics) *F From: eberl@rascal.med.harvard.edu *F Subject: Re: Help FlyBase - auditory mutants *F Content-Length: 2762 *F Dear Eleanor, *F I am in principle in favor of using a systematic naming scheme for these *F mutants, but I need to confer with the other authors about what would work *F well. I can right away that ad (for auditory defective) may be misleading, *F because it implies that these mutants are defective in the sensory part of *F the auditory behavior pathway. In fact the majority are defective in *F downstream parts of the pathway (central or motor). So something like acd *F (auditory courtship defective) might be more appropriate. But I would like *F to have a chance to think about this a little more and discussing it with *F colleagues before locking myself in. As I'm sure you will agree, the name *F can be quite important. *F Though we haven't published it yet, we have already renamed one of the *F mutants (5P1) as beethoven (btv). I think this is not a problem because as *F we learn more about these mutations, it is likely that we would replace the *F generic name with a more descriptive name anyway. *F Finally, these mutants are all on the second chromosome. We have not *F screened any of the other chromosomes yet. *F How much of a hurry are you in to get this done? I hope to be able to get *F back to you in a week or so. *F Until later, *F Dan *F From eleanor@gen.cam.ac.uk Mon Feb 16 07:50:08 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 16 Feb 1998 07:50:08 +0000 *F To: eberl@rascal.med.harvard.edu *F Subject: Re: Help FlyBase - auditory mutants *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Mon, 16 Feb 1998 07:58:09 +0000 *F Content-Length: 2360 *F Hi Dan, *F >I am in principle in favor of using a systematic naming scheme for these *F >mutants, but I need to confer with the other authors about what would work *F >well. *F Marvellous, it seemed to me that I should offer you the chance to think *F about a naming system as all these mutants are new to the database. *F >I can right away that ad (for auditory defective) may be misleading, *F >because it implies that these mutants are defective in the sensory part of *F >the auditory behavior pathway. In fact the majority are defective in *F >downstream parts of the pathway (central or motor). So something like acd *F >(auditory courtship defective) might be more appropriate. *F I simply threw in ad (for auditory defective) for a suggestion, it is *F entirely up to the group what you decide upon. We will accept whatever you *F suggest. *F >Though we haven't published it yet, we have already renamed one of the *F >mutants (5P1) as beethoven (btv). I think this is not a problem because as *F >we learn more about these mutations, it is likely that we would replace the *F >generic name with a more descriptive name anyway. *F Indeed, as for pir also. *F Could I use the information that 5P1 is btv for curation of your paper? *F All data you submit that is over and above that present in the paper will *F be curated as a personal communication from yourself to FlyBase, then we *F have a source for the information you provide if anyone requests it. Is *F that OK with you? *F >How much of a hurry are you in to get this done? I hope to be able to get *F >back to you in a week or so. *F That is fine with me. If the delay will be longer please let me know. We *F have a monthly collection of data for loading into the public files. The *F next collection should be at the beginning of March so I would like this *F issue completely resolved by then so there is no delay in your data being *F released. *F Thanks for your help and I look forward to hearing from you soon. *F Regards, *F Eleanor Whitfield *F FlyBase *F From eberl@rascal.med.harvard.edu Mon Feb 16 17:54:19 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 16 Feb 1998 17:54:19 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Transfer-Encoding: binary *F Date: Mon, 16 Feb 1998 13:05:03 -0400 *F To: Eleanor Whitfield (Genetics) *F From: eberl@rascal.med.harvard.edu *F Subject: Re: Help FlyBase - auditory mutants *F Content-Length: 432 *F Dear Eleanor, *F It seems that all the authors are in agreement with acd as an appropriate *F symbol, for auditory courtship defective. *F Regarding beethoven, I think it should be fine to make the entry that 5P1 *F is renamed btv, and furthermore its map position can now be defined as 36E, *F on the basis of its inclusion in Df(2L)TW119 and Df(2L)TW201. It *F complements rdo, which is in the same genetic interval. *F Thank you very much. *F Dan # *U FBrf0100703 *a Gonzalez-Reyes *b A. *t 1997.2.17 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Fri Feb 13 14:56:12 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 13 Feb 1998 14:56:12 +0000 *F To: agr@mole.bio.cam.ac.uk *F Subject: Help FlyBase - fs(3)225-19 *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 13 Feb 1998 15:04:09 +0000 *F Content-Length: 922 *F Hi, *F I am curating a paper of yours for FlyBase: *F FBrf0100046 == Gonzalez-Reyes et al., 1997, Development 124(24): 4927--4937 *F in which you discuss mutation fs(3)225-19 (described by Tearle and *F Nusslein-Volhard) being allelic to the gene spn-D. *F The information in the Red Book suggests fs(3)225-19 is in fact allelic to *F spn-B (allele spn-B3). *F Have you made an error or are you correcting data in the Red Book? *F Please contact me as soon as possible regarding this matter, *F Regards, *F Eleanor Whitfield *F FlyBase *F \-------------------------------------------------------------- *F Eleanor Whitfield. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email : eleanor@gen.cam.ac.uk *F Cambridge, CB2 3EH, Phone : 44 (0)1223-333963 *F UK. FAX : 44 (0)1223-333992 *F \-------------------------------------------------------------- *F From agr@mole.bio.cam.ac.uk Tue Feb 17 10:54:35 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 17 Feb 1998 10:54:35 +0000 *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F To: Eleanor Whitfield (Genetics) *F From: Acaimo Gonzalez-Reyes *F Subject: Re: Help FlyBase - fs(3)225-19 *F Date: Tue, 17 Feb 1998 11:02:39 +0000 *F Content-Length: 647 *F Dear Eleanor, *F Following our telephone conversation, I am writing to you to confirm that *F fs(3)225-19 is indeed allelic to spn-D, and not to spn-B as stated in the *F Red Book. I may add that I have done the complementation tests of the *F different spn genes with 225-19 and found only spn-D to be allelic to *F 225-19. *F Best regards, *F Acaimo *F \--------------------------------------------------------- *F Acaimo Gonzalez-Reyes Tel: +44 (0)1223 334 113 *F Wellcome/CRC Institute Fax: +44 (0)1223 334 089 *F Tennis Court Road agr@mole.bio.cam.ac.uk *F Cambridge CB2 1QR (UK) *F \--------------------------------------------------------- # *U FBrf0100735 *a Bloomington Drosophila Stock Center *b ?. *t 1998.3.2 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Alleles from Carl Thummel *F Date: 2 March 1998 *F *F Information communicated: *F *F Allele symbol HR78[2] *F Map location chromosome 3 *F Donor of stock Carl Thummel *F *F Allele symbol l(3)E2[1] *F Map location chromosome 3 *F Donor of stock Carl Thummel *F Comments Complementation group near HR78 (78D4-5), per C. Thummel *F *F Allele symbol l(3)E22[1] *F Map location chromosome 3 *F Donor of stock Carl Thummel *F Comments Complementation group near HR78 (78D4-5), per C. Thummel *F # *U FBrf0100811 *a Philp *b A.V. *t 1998.2.3 *T personal communication to FlyBase *u *F From ajvphilp@scotlandmail.com Tue Feb 03 20:21:54 1998 *F Subject: l(2)k02807 allelic to ab *F \-- l(2)k02807 is allelic to abrupt (ab1D) by complementation testing *F (0/58) *F \+++++++++++++++++++++++++this is a message from+++++++++++++++++++++++ *F Alastair Valentine Philp MA (Cantab) PhD (Dundee) *F Mail Code 0683, Rm334 CMM-W *F Howard Hughes Med Institute/ Div of Cellular and Molecular Medicine *F University of California, San Diego *F La Jolla CA 92093-0683 *F Telephone:+1 619 534 9703 *F Telefax: +1 619 534 9701 *F preferred e-mail address: ajvphilp@scotlandmail.com *F www: http://www.geocities.com/CapeCanaveral/Lab/6100/ *F ================================================================== # *U FBrf0100812 *a Noll *b M. *t 1998.2.10 *T personal communication to FlyBase *u *F From noll@molbio2.unizh.ch Tue Feb 10 18:41:21 1998 *F To: Rachel Drysdale (Genetics) *F >Hi Marcus, *F > *F >FlyBase has been going back in time, over some older publications, *F >gathering some historic data for our genes files. In 1990 you published: *F >*x FBrf0051757 == Schneitz and Noll, 1990, Experientia 46(Abstr.): A82 *F > *F >in which you mentioned these paired type homeobox genes: *F >*a L13-4 *F >*a Pph13 *F >*a Pph25 *F > *F >My question is, have these been published since under different symbols? *F >If so we can amalgamate the information, and avoid having more FlyBase gene *F >records than there are genes. *F > *F >many thanks for your help, *F > *F >Rachel. *F > *F Dear Rachel, *F two of the three paired-type homeobox genes you mentioned are *F published: *F L13-4 is aristaless (al). The name L13-4 should actually be lambda13-4, *F which is the name of the genomic phage covering aristaless, as shown in our *F publication by Schneitz et al., Genes & Dev. 7, 114-129 (1993). The *F GenBank accession number of the published sequence is L08401. *F PPH25 is the same as D-goosecoid (D-gsc) and has been published by Hahn and *F Jaeckle, EMBO J. 15, 3077-3084 (1996). Its GenBank accession number is *F U52968. Our unpublished work locates this gene to chromosomal band 21D1,2 *F and orients it on the chromosome such that the direction of its *F transcription is oriented towards the telomere. *F PPH13 is still unpublished until someone will publish it for us! It is *F located immediately distal to PPH25 (in the neighbouring RI-fragment), i.e. *F D-gsc, and its transcription occurs in the opposite orientation to that of *F D-gsc, i.e. the 3' ends of D-gsc and PPH13 are oriented towards each other. *F The homeodomain of PPH13 is also of the paired-type. *F I hope this clarifies matters somewhat. *F With best regards, *F Markus # *U FBrf0100813 *a Meister *b M. *t 1998.2.10 *T personal communication to FlyBase *u *F >From meister@astorg.u-strasbg.fr Tue Feb 10 06:40:16 1998 *F Dear Kathy, *F P1587 is allelic to P1707 and maps at 62E6-7 (not at 66 as indicated): we *F checked it both by in situ hybridization and by complementation studies. *F Actually, we have cloned the corresponding gene and are about to publish it. *F Hope this is useful for you. *F With best wishes, *F Marie *F Marie Meister *F UPR 9022 du CNRS, IBMC *F 15 rue Rene Descartes *F 67084 Strasbourg cedex *F Tel 03 88 41 70 32 *F e-mail : meister@ibmc.u-strasbg.fr # *U FBrf0100814 *a Rodriguez *b A. *t 1998.2.14 *T personal communication to FlyBase *u *F From rodrig02@UTSW.SWMED.EDU Sat Feb 14 21:44:22 1998 *F Subject: Re: Dredd gene correction *F Hello Rachel, *F As you might recall I had communicated to you information regarding the *F Dredd gene at 1B12 some time ago. We have (just this month) submitted the *F first paper on Dredd, and have named the gene: Death related *F ced-3/Nedd2-like protein. Sorry for our initial mishap on GenBank using *F Drosophila as the prefix, but hopefully this will allow Dredd to remain as *F the symbol name instead of Redd. Thank you in advance. *F Antony *F Antony Rodriguez *F UT Southwestern Med. Ctr *F Dept. of Cell Biology & Neuroscience *F 5323 Harry Hines Blvd. *F Dallas, TX 75235 # *U FBrf0100815 *a Roote *b J. *c M. *d Ashburner *t 1998.2.17 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Thu Feb 12 15:22:03 1998 *F Subject: c163.41 *F Rachel, *F Michael had a quick chat re C163. *F He thinks it should go in as a beat allele and br48 and 35Eb should be beat *F synonyms. Not necessary to have 35Eb as a separate entry. *F BFN, *F John # *U FBrf0100816 *a Roote *b J. *t 1998.1.27 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Jan 27 10:47:22 1998 *F To: Rachel Drysdale (Genetics) *F >Hi John, *F > *F >*x FBrf0098381 == The Moscow Regional Drosophila melanogaster Stock *F >Center, Dubna, Russia, 1997, D. I. S. 80: 109--130 *F > *F >Mentions *F > *F >2050 In(2L)C163, l(2)br48AM4=l(2)35Eb, l(2)CA7/CyO *F > *F >all is OK bar the 'l(2)CA7' part. *F > *F >They (Moscow) know nothing about it (except that it came from here). Do *F >you? *F > *F >ta *F > *F >Rachel. *F Rachel, *F I think CA7 *F may have been the lethality of wbPA43/In(2L)C163.41 *F From rd120@gen.cam.ac.uk Tue Feb 17 10:53:44 1998 *F Are you happy for me to invent l(2)CA7PA43 and l(2)CA7C163 on the basis *F of what you wrote? *F Rachel *F From jr32@mole.bio.cam.ac.uk Tue Feb 17 11:16:17 1998 *F yes it's fine by me. # *U FBrf0100817 *a Roote *b J. *t 1998.2.17 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Tue Feb 17 12:59:40 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Me *F Rachel, *F We have observed that the Me phenotype of LS(3)267, Me//DS(3)P10, Ki is *F suppressed (but not wild-type), whereas LS(3)267, Me//DS(3)267 is *F full-blown Me. This suggests that Me is in the interval duplicated by *F 267L (LS(3)267) P10R (DS(3)P10). *F J # *U FBrf0100818 *a Bloomington Drosophila Stock Center *b ?. *t 1998.2.18 *T personal communication to FlyBase *u *F From: Rachel Drysdale (Genetics) *F Date: Wed, 18 Feb 1998 11:53:22 +0000 *F B-#1221 In(3LR)267/TM6B, Tb+ *F Breakpoints: 065D01-02;081F *F Donor: Caltech Stock Center Donor's source: Loring Craymer # *U FBrf0100819 *a Noll *b M. *c W. *d McGinnis *t 1998.2.19 *T personal communication to FlyBase *u *F From noll@molbio2.unizh.ch Wed Feb 11 15:37:54 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 11 Feb 1998 15:37:54 +0000 *F >Hi Markus, *F > *F >I had a thought. I wonder if your PPH13 corresponds to 60Mun2, which has *F >been molecularly mapped to be next to Gsc (=PPH25). *F > *F >Gsc corresponds to '60Mun1' published by Dessain and McGinnis *F >(FBrf0065373). In this paper they discuss '60Mun1'(=Gsc/PPH25) and 60Mun2, *F >on page 26. (They also say both genes are at 60C but this turned out to be *F >a case of mistaken telomeres). The FlyBase gene record for 60Mun2 is at *F >the bottom of this message. I can send you a copy of the relevant pages of *F >the article, or all of it if you like, if you don't have a copy. *F > *F >60Mun2 does not have a sequence accession number but the paper gives a *F >short amount of amino acid (not DNA, sadly) sequence. It is *F > *F >VWFK NRRAKWRKQ KREEQE *F > *F >and falls in the III/VI region of the homeodomain. *F > *F >The authors do not say that this is a prd class homeodomain, but I do not *F >know whether anyone could, on the basis of such a short sequence (I am not *F >a sequence expert by any stretch of the imagination, when I saw the *F >transcript of my PhD gene was going on for 10kb I put my pipettes down and *F >wrote up!). *F > *F >They do say *F >'The 60Mun1 (i.e. Gsc/PPH25) partial homeobox sequence maps 4kb 3' to the *F >60Mun2 homeobox, in the same 5' to 3' orientation.....' *F > *F >so that suggests that its going the opposite way. But I really don't know *F >how carefully this was done, especially given that no-one has published on *F >it since, and the cytology at least was apparently not done very *F >carefully. *F > *F >What do you think? *F >Is it possible to tell anything from such a short run of amino acid *F >sequence? Does it look familiar to you? Given what you know and what *F >Dessain and McGinnis say, it is possible that PPH13 could lie between Gsc *F >and 60Mun2, having escaped detection, or is it more likely that PPH13 *F >corresponds to 60Mun2 and that the statement about the orientation of *F >60Mun2 in FBrf0065373 is wrong? *F > *F >Hope this is not a nuisance, but if we can merge PPH13 with 60Mun2 its one *F >less problem on the genome map. Every little bit helps. *F > *F >Rachel. *F > *F > *F Dear Rachel, *F I will contact Bill McGinnis about your query. The sequence you *F sent me is identical to that of gsc, which is our PPH25, but different from *F PPH13. So Bill may have confused his two genes although it is true that the *F two 3' ends of PPH13 and gsc are only a few kbs apart. I will sort things *F out with Bill and let you know about the outcome. *F Over ten years ago, one of my diploma students, Roland Rutschmann, *F isolated the genomic region that included both PPH13 and PPH25 by low *F stringency hybridization with the homeobox of the prd gene (PvuII-PstI *F fragment; hence P(vuII-)P(stI)H(omology) 13 and 25!) and sequenced the *F regions that hybridized with it, the homeoboxes of PPH13 and PPH25. While *F he had sequenced the entire homeobox of PPH13 (it includes an intron after *F the fifth amino acid of the homeodomain), he was unable to locate the last *F 14 amino acids of PPH25, i.e. of gsc, because they are encoded on a *F separate exon and we had no cDNAs. Therefore, we do not know how close the *F two 3' ends of PPH13 and gsc are but they are closer than 3-4 kb, which is *F the distance of the portions of the two homeoboxes that we have sequenced. *F With best regards, *F Markus *F From noll@molbio2.unizh.ch Thu Feb 19 12:26:54 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Dear Rachel, *F A week ago, I have sent the following message to Bill McGinnis *F (together with a copy of your message to me): *F Dear Billy, *F Rachel Drysdale sent me this message yesterday. I promised to *F contact you to clear things up. Your sequence mentioned above (VWFK *F NRRAKWRKQ KREEQE) is identical to that of gsc, which is our PPH25, but *F different from PPH13. So, if this sequence was derived from your 60Mun2 *F gene, then it must be the same as gsc (PPH25) and, accordingly, your 60Mun1 *F gene would correspond to our PPH13 gene. *F Over ten years ago, one of my diploma students, Roland Rutschmann, *F isolated the genomic region that included both PPH13 and PPH25 by low *F stringency hybridization with the homeobox of the prd gene (PvuII-PstI *F fragment; hence P(vuII-)P(stI)H(omology) 13 and 25!) and sequenced the *F regions that hybridized with it, the homeoboxes of PPH13 and PPH25. While *F he had sequenced the entire homeobox of PPH13 (it includes an intron after *F the fifth amino acid of the homeodomain), he was unable to locate the last *F 14 amino acids of PPH25, i.e. of gsc, because they are encoded on a *F separate exon and we had no cDNAs. Therefore, we do not know how close the *F two 3' ends of PPH13 and gsc are but they are closer than 3-4 kb, which is *F the distance of the portions of the two homeoboxes that we have sequenced. *F We know for sure that the two homeoboxes are oriented in opposite *F directions with their 3' ends towards each other. Thus, the direction of *F transcription of the proximal PPH25 gene is oriented towards the telomere *F whereas that of the distal PPH13 gene is oriented towards the centromere. *F It seems clear to me that we are looking at the same genes, i.e., *F that PPH13 and PPH25 correspond to 60Mun1 and 60Mun2, because PPH25 is *F identical to gsc and one of your genes (which one?) while the other, PPH13 *F (which one of yours?), is located at the same distance from the 3' end of *F gsc although we seem to have a discrepancy in its orientation. *F The homeodomain sequence of PPH13 is: *F QRRYR TTFNTLQLQELERAFQRTHYPDVFFREELAVRIDLTEARVQVWFQNRRAKWRNEE *F with the space indicating the location of a 121 bp intron (after position *F 14 of the homeobox sequence). If you have sequenced both Mun1 and Mun2, it *F should be easy to verify which corresponds to PPH13 and which to gsc. *F Thank you very much for taking the time to look into that. *F With best regards, *F Markus *F Bill's answer: *F Mime-Version: 1.0 *F Date: Mon, 16 Feb 1998 12:07:38 -0800 *F To: Markus Noll *F From: mcginnis@biomail.ucsd.edu (William McGinnis) *F Subject: pph13 *F Status: *F Markus, *F Hi, I went back and looked over the sequence information we have on the *F 21C homeobox genes. *F It seems nearly sure that PPH13 is identical to what we called 60Mun1 in *F Dessain and McGinnis in 'Advances in Developmental *F Biochemistry' Vol. 2, pp. 1-55. The aa sequence that you sent matches *F 60Mun1 at every position from +6 of your sequence to beyond the WFQNRR *F motif so there seems little doubt. And we derived our sequence completely *F from genomic DNA so the presence of an intron near the 5' end would explain *F why the aa sequences diverge at that point. The homeobox sequences (i.e. *F entire 60Mun1 and the C terminal half of the 60Mun2 homeobox), mapped about *F 6 kb apart on Dessain's phage map. *F . *F . *F 60Mun2 is identical to goosecoid/PPH25, in fact Desplan et al used the *F 60Mun2 clone that we sent them to isolate the gene. *F Hope this is helpful to you, *F Bill *F In summary, it is clear that gsc is identical to our PPH25 and to *F Bill's 60Mun2 gene, which is the proximal gene of the pair of neighboring *F homeobox genes at 21C8/D1, and our PPH13 is identical to Bill's 60Mun1 (not *F 60Mun2!) gene. The two genes are convergently transcribed, i.e. the distal *F PPH13 gene from left to right, the proximal gsc from right to left. Their *F 3' ends are separated by less than about 4 kb. Bill's answer is clearly *F not consistent with what you wrote they have published in their paper in *F 'Advances in Developmental Biochemistry' Vol. 2, pp. 1-55. Since our *F library does not carry this journal, I could not check myself what they *F wrote on page 26. *F . *F . *F . *F If you like, you can submit our sequence of the PPH13 homeodomain *F as personal communication to FlyBase. Would you like to obtain the genomic *F DNA sequence of it (including the 121 bp intron after position 14)? *F With best regards, *F Markus # *U FBrf0100820 *a Gubb *b D. *t 1998.2.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Feb 25 10:14:46 1998 *F To: rd120@gen.cam.ac.uk, dg27@gen.cam.ac.uk *F Subject: pk update *F bits of prickle news for FlyBase: *F Tp(2;3)pk-sple-24 has no molecular lesions within the pk-sple gene therefore *F mutant phenotype implies a position effect. *F 'CyO-G': the chromosome represented by Tp(2;2)pk-sple26 is a nice second *F chromosome balancer being marked with pkpk-sple-26, an easy to score *F visible marker. Probably a better balancer than CyO because of its *F increased cytological complexity. *F The pk78a, pk78b, pk78d, pk78f, pk78g, pk78l *F and pk78m chromosomes are lost. *F pk78t = pk29 and is caused by T(2;3)pk78t. *F from David. # *U FBrf0100821 *a Russell *b S. *t 1998.3.2 *T personal communication to FlyBase *u *F From sr120@mole.bio.cam.ac.uk Mon Mar 02 09:57:27 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 2 Mar 1998 09:57:27 +0000 *F Dear Rachel, *F I have been perusing the genes entry for Dichaete in FlyBase and notice *F that the designation loD (lethal of Dichaete) is still in use. It is now *F absolutely clear that all alleles designated loD (loD3, loD4, loD6, *F loD7, loD8, loD10 and loDLG9 are alleles of Dichaete. For the *F dominant alleles (3,4,6,7 and 10) these are associated with breaks in 3' *F regulatory sequences which result in the tissue specific loss of Dichaete *F expression in the embryo. (loD10 = In(3L)D10 70A1-2;70D1-2D). Dr8 *F is a similar rearrangement with a 3' break but has no dominant phenotype. *F All of these alleles are lethal in combination with point alleles of *F Dichaete, e.g Dr72 and small deletions which only remove Dichaete *F sequences such as Dr513. *F The point allele Dr72, near null protein levels, is phenotypically null in *F segmentation. *F The small deletion Dr513 deletion from p-element P{PZ}rJ375 removes 5' half of *F Dichaete transcription unit. *F Best wishes *F Steve *F \-------------------------------------------------------------------------------- *F Dr Steven Russell sr120@mole.bio.cam.ac.uk *F Department of Genetics *F University of Cambridge Lab (+44) 01223 333970 *F Downing Street Office (+44) 01223 333967 *F Cambridge *F CB2 3EH Fax (+44) 01223 333992 *F \-------------------------------------------------------------------------------- # *U FBrf0100822 *a Bloomington Drosophila Stock Center *b ?. *t 1998.3.2 *T personal communication to FlyBase *u *F >From kcook@bio.indiana.edu Fri Feb 27 16:40:29 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Subject: More on Sxl alleles *F Rachel-- *F Lindsley and Zimm listed Sxlf4 and f5 as new names for fs(1)M1061 amd *F fs(1)M1062 and cited FBrf0040657 and FBrf0044470, but left it unstated *F that 1 = 12-222 = f4 and 2 = 13-558 = f5. *F The fs1 and fs2 names slipped into a couple of papers before Lindsley and *F Zimm came out, but not with the details needed to make the connections. *F The fs1 and fs2 names didn't make it into L and Z. *F As far as I am aware there is nothing published that says explicitly *F 'Mohler called 12-222 Sxlfs1 and 13-558 Sxlfs2', though he did. *F To summarise for f4: *F Sxlf4 = Sxlfs1 = fs(1)M10612-222 = fs(1)M1061 = Sxlfs#1 = Sxlfs *F Later, *F Kevin # *U FBrf0100823 *a Ashburner *b M. *t 1998.3.3 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Mon Mar 02 14:39:45 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Rachel *F A communication to FlyBase. *F I have just checked some pictures of Df(1)ras59 heterozygotes *F made by Yong Zhang. *F FB has: *F \*B 9E1;9F10-9F11 *F \*E FBrf0044450 == rd2164 == Janca et al., 1986, Genetics 112: 43--64 *F \*B ??? 9A;9F10-9F11 *F \*B ??? 9A;9F10-9F11 *F \*B 9E1;9F10-9F11 *F \*E FBrf0082455 == ew4069 == Pauli et al., 1995, D. I. S. 76: 107--124 *F \*B 9E1;9F10-9F11 *F my reading of the chromosomes is that: *F \*B 9A;9F10-9F11 *F is correct. *F Michael Ashburner # *U FBrf0100824 *a Beaton *b A. *t 1998.3.3 *T personal communication to FlyBase *u *F > From abeaton@uclink4.berkeley.edu Wed Nov 05 23:20:28 1997 *F > >From ag24@gen.cam.ac.uk Mon Oct 27 13:26:07 1997 *F > >>From abeaton@uclink4.berkeley.edu Sun Oct 26 01:45:44 1997 *F > >> >From ag24@gen.cam.ac.uk Fri Oct 17 23:15:02 1997 *F > *F > >> >PC-ness conflicts/queries *F > >> >01528 05287 PC bloom has both - reserve 01528? *F > >> *F > >> this one's a bitch. Lasko thinks 01528 lethal is elsewhere. like 39E. I *F > >> don't know. Roote says discard it. the problem was that k08613 noncomps *F > >> both 01528 and 05287. *F > > *F > >In that case I should get Kathy to put a note in the stock listing for *F > >01528 saying this. Do you mean Roote says discard 01528? *F > *F > Yes a note from Lasko, the lethality for the chromosome 'l(2)01528 looks *F > like 39E because outside TW84 but inside TW65 (and TW161)'. Roote or some *F > buddy of his David Huen, couldn't assign it in 38F so they too thought it *F > elsewhere. # *U FBrf0100825 *a Petitt *b M. *t 1998.3.3 *T personal communication to FlyBase *u *F From petitt@rascal.med.harvard.edu Mon Mar 02 19:24:22 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 2 Mar 1998 19:24:22 +0000 *F Subject: Flybase entry for 'fray' *F To Whom It May Concern- *F fray was reported originally in Perrimon et al. (1996) Genetics 144(4): *F 1681-1692 as a P induced mutation l(3)07551 and is allelic to a second P *F element lethal called s2427. *F Matt Petitt *F (Perrimon Lab) *F Dept. of Genetics *F Harvard Medical School # *U FBrf0100826 *a Roote *b J. *t 1998.3.4 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Mar 04 19:53:38 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Subject: b79h1A *F Rach, *F Update: b79h1A is deleted 34Db, Sos, black but is leaky for 34Dc (may be PEV). *F Rootles # *U FBrf0100827 *a Beaton *b A. *c T. *d Laverty *t 1998.3.6 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Fri Mar 06 10:43:42 1998 *F Subject: new cytology *F from Amy Beaton: *F >Computer folks have generated a list of Todd's cytology that are not kosher *F >Recent cytology revisions of importance to you: *F > *F >k10011 that Su(H) Todd says it is 35B8-10 not 35B10-11 (11 doesn't exist *F >in Sorsa) *F > *F >k03505 36F11-12 revised to 35F11-12 98.3.5 # *U FBrf0100828 *a Roote *b J. *t 1998.3.6 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Fri Mar 06 13:32:46 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 6 Mar 1998 13:32:46 +0000 *F >is fs(2)ltoRJ36 to become known as ? *F It's true, Denise Montell and her student Rebecca Tinker are working on *F courgette = fs(2)ltoRJ36. It was originally called no-relish because *F Rebecca thought it cleaved (clove?) gurken, but this is now not the case. *F It is genetically inseparable from vasa and so may be vig or the other gene *F nested within vasa. It and vasa are within the same ~4kb region. We have *F a P in vig (ep(2)0812) so will soon know if cgt = vig. *F John # *U FBrf0100829 *a Spradling *b A. *t 1997.8.22 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Allan Spradling *F to: Bloomington Drosophila Stock Center *F Subject: EP lines from BDGP *F Date: 22 August 1997 *F *F Information communicated: *F *F "w[1118] P{w}EP307/FM7a","9D1-2" *F "w[1118] P{w}EP308/FM7a","10F1-2" *F "w[1118] P{w}EP317/FM7a","16F7-8" *F "w[1118] P{w}EP319/FM7a","9F1-2" *F "w[1118] P{w}EP325/FM7a","7D16-17" *F "w[1118] P{w}EP334/FM7a","12F4-5" *F "w[1118] P{w}EP335/FM7a","2B16-18" *F "w[1118] P{w}EP345/FM7a","10E1-2" *F "w[1118] P{w}EP346/FM7a","19A1-2" *F "w[1118] P{w}EP347/FM7a","7C4-5" *F "w[1118] P{w}EP351/FM7a","3E1-2" *F "w[1118] P{w}EP355/FM7a","14B15-16" *F "w[1118] P{w}EP356/FM7a","1B7-8" *F "w[1118] P{w}EP362/FM7a","9E7-8" *F "w[1118] P{w}EP367/FM7a","19A1-2" *F "w[1118] P{w}EP369/FM7a","1C1-3" *F "w[1118] P{w}EP379/FM7a","9B11-12" *F "w[1118] P{w}EP382/FM7a","1D1-2" *F "w[1118] P{w}EP385/FM7a","12B1-2" *F "w[1118] P{w}EP390/FM7a","5C1-2" *F "w[1118] P{w}EP395/FM7a","10D4-5" *F "w[1118] P{w}EP399/FM7a","12F4-5" *F "w[1118] P{w}EP400/FM7a","14B5-8" *F "w[1118] P{w}EP404/FM7a","5B6-7" *F "w[1118] P{w}EP406/FM7a","10E1-2" *F "w[1118] P{w}EP425/FM7a","4D6-7" *F "w[1118] P{w}EP426/FM7a","2F1-2" *F "w[1118] P{w}EP427/FM7a","2C1-2" *F "w[1118] P{w}EP438/FM7a","17D5-6" *F "w[1118] P{w}EP439/FM7a","07D3-4" *F "w[1118] P{w}EP442/FM7a","05E4-5" *F "w[1118] P{w}EP443/FM7a","17C1-2" *F "w[1118] P{w}EP444/FM7a","05C5-8" *F "w[1118] P{w}EP452/FM7a","01B7-8" *F "w[1118] P{w}EP491/FM7a","13C5-6" *F "w[1118] P{w}EP495/FM7a","18C6-9" *F "w[1118] P{w}EP496/FM7a","05A10-13" *F "w[1118] P{w}EP514/FM7a","12B4-5" *F "w[1118] P{w}EP555/FM7a","19F1-2" *F "w[1118] P{w}EP556/FM7a","09B7-8" *F "w[1118] P{w}EP620/FM7a","14B5-8" *F "w[1118] P{w}EP760/FM7a","09F1-2" *F "w[1118] P{w}EP764/FM7a","12E4-5" *F "w[1118] P{w}EP766/FM7a","18D5-8" *F "w[1118] P{w}EP767/FM7a","02C1-2" *F "w[1118] P{w}EP770/FM7a","14B7-10" *F "w[1118] P{w}EP773/FM7a","01E3-4" *F "w[1118] P{w}EP779/FM7a","10D4-5" *F "w[1118] P{w}EP784/FM7a","12E5-6" *F "w[1118] P{w}EP790/FM7a","12C1-2" *F "w[1118] P{w}EP804/FM7a","03A5-6" *F "w[1118] P{w}EP805/FM7a","12B1-2" *F "w[1118] P{w}EP908/FM7a","19A5-6" *F "w[1118] P{w}EP911/FM7a","03E6-7" *F "w[1118] P{w}EP912/FM7a","08E10-11" *F "w[1118] P{w}EP950/FM7a","08F8-10" *F "w[1118] P{w}EP953/FM7a","15-A4-6" *F "w[1118] P{w}EP964/FM7a","01E3-4" *F "w[1118] P{w}EP970/FM7a","16F1-4" *F "w[1118] P{w}EP971/FM7a","18F4-5" *F "w[1118] P{w}EP973/FM7a","3C11-12" *F "w[1118] P{w}EP975/FM7a","13B1-2" *F "w[1118] P{w}EP977/FM7a","12F4-5" *F "w[1118] P{w}EP982/FM7a","09B7-8" *F "w[1118] P{w}EP991/FM7a","18C1-2" *F "w[1118] P{w}EP1007/FM7a","13C2-3" *F "w[1118] P{w}EP1016/FM7a","14B13-16" *F "w[1118] P{w}EP1023/FM7a","11F6-7" *F "w[1118] P{w}EP1029/FM7a","07C4-5" *F "w[1118] P{w}EP1030/FM7a","08C4-5" *F "w[1118] P{w}EP1031/FM7a","15F1-5" *F "w[1118] P{w}EP1033/FM7a","07D5-7" *F "w[1118] P{w}EP1036/FM7a","18D5-9" *F "w[1118] P{w}EP1071/FM7a","13F14-17" *F "w[1118] P{w}EP1073/FM7a","14B11-14" *F "w[1118] P{w}EP1075/FM7a","13F14-17" *F "w[1118] P{w}EP1083/FM7a","05D1-4" *F "w[1118] P{w}EP1088/FM7a","13F1-2" *F "w[1118] P{w}EP1090/FM7a","01E3-4" # *U FBrf0100830 *a Rorth *b P. *t 1998.4.4 *T personal communication to FlyBase *u *F From rorth@mail1.ciwemb.edu Sat Apr 4 17:33:15 1998 *F Date: 4 Apr 1998 17:30:52 -0500 *F From: "Pernille Rorth" *F Subject: Re: EP sequence *F To: "Madeline Crosby" *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 2454 *F *F RE>EP sequence 4/4/98 *F *F Dear Lynn *F *F I've indicated below answers/comments about the EP sequence. I can also fax *F you a handdrawn detailed map of the plasmid if you'd like. *F *F *F *F Segment 1: OK: the 5'end of P{CaSpeR-4}, including the white gene. (We have *F this sequence.) *F P: OK *F *F Segment 2: OK: the CaSper-4 polylinker up to (and including) the NotI site. *F (We have this sequence.) *F P: OK *F *F Segment 3: ? My guess: the pHSS7 sequences from P{PZ} and related *F constructs;excised by NotI. (We have this sequence.) *F ? Which orientation is it in?? *F P: Yes, this is pHSS7. Orientation is such that the Pst1 site in the kana *F gene is 1.7 kb from the unique EcoR1 site in EP *F *F (Segment 4: Starts with the CaSpeR-4 polylinker, picking up from NotI site. *F ? How far does it go (what did you use to cut the enhancer *F segment out of pBluescript?) *F ? Need details on ligation to GAGA-UAS sequences; where you put *F the StyI site in pBluescript, etc. *F *F Segment 5: ? Constructed enhancer ) *F *F P: The "enhancer" in EP starts from the NotI site (so no return to pCasper *F polylinker) and contains both GAGA sites and thereafter Gal4 sites. It ends *F in Sal1, which is then joined to XhoI in the next segment. In detail: *F GCGGCCGCTCTAGCCCCCCTCGAATGTTCTCTCTCTTCTCTTCTCTCTCTCTTTCTCGAATGTTCTCTCTCTTCT *F CTTCTCTCTCTCTTTCTCGAGGTCATCAAGCTTAGGCCTC(a-oligo)(b-oligo)(b-oligo)(b-oligo) *F (a-oligo)(b-oligo)(a-oligo)GTCGAG *F *F I've written the sequence as is, because the whole thing was made in an *F (overly) complicated way - the last 6 nt are the Sal1/Xho1 junction *F *F each a or b oligo has 2 Gal4 sites and spacer sequence: *F a-oligo:CAAGGCGGAGTACTGTCCTCCGGGCTGGCGGAGTACTGTCCTCCGG *F b-oligo:CAAGGTCGGAGTACTGTCCTCCGACACTAGAGGTCGGAGTACTGTCCTCCGACG *F *F For the next segment (hsp70 promoter), the promoter fragment was first cloned *F into another polylinker and then to pCasper thus adding a Kpn1 site upstream *F and and a Bgl2 site downstream of the promoter before Pst1, Hind3 in pCasper *F (at the 3'P end). *F The sequence goes: Sal1/Xho1 junction (see above)- *F GGTACC(GAGAGAGCGCCGGAGTAT.....CAAGCAAAGTGAACACGTCG)AGATCT-Pst1 *F The sequence in the parenthesis is the hsp70 promoter which you have the *F sequence for. *F *F *F *F Segment 9: OK: the 3' end of P{CaSpeR-4} (We have this sequence.) *F P: Yes, from the Pst1 site. *F *F *F Going through a lot of old notebooks to refresh.... good to get it done. Let *F me know if you need more info. *F *F regards *F *F Pernille Rorth *F # *U FBrf0102049 *a Merriam *b J. *c M.T. *d Yamamoto *e B. *f Stewart *g M.R. *h Rahman *i T. *j Nicolau *t 1998.4.24 *T personal communication to FlyBase *u X chromosome segmental aneuploidy and response to gene dosage in Drosophiola melanogaster. *F Archived. # *U FBrf0102051 *a Pollock *b J. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:51:58 1998 *F To: john.pollock@andrew.cmu.edu *F Subject: ADRC: 549A *F Dear John, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'helmsman Encodes A Complement-like Protein Involved in Cell Elongation in *F Tracheal and Neuronal Development.' *F You describe hlm, a gene new to FlyBase. Do you have a cytological *F location for hlm? It is nice if we can keep as many gene records anchored *F to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jp4o+@andrew.cmu.edu Tue Mar 10 13:15:01 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Subject: Re: ADRC: 549A *F Rachel: *F helmsman was previously called N63. It maps to 76C2-3. It encodes a *F transcript of 2177 nt encoding a peptide of 558 aa. *F For your information, a neighboring ORF found in the same lambda clone *F produces a transcript of 7 kb that is expressed in the embryonic *F salavary gland. No further analysis of this second ORF or its DNA has *F been carried out. *F Let me know if I can be of any further assistance. *F John *F John Archie Pollock, Ph.D. *F Associate Professor *F Department of Biological Sciences *F Carnegie Mellon University *F 4400 Fifth Avenue *F Pittsburgh, PA 15213 *F 412 - 268 - 6181 Office *F 412 - 268 - 6164 Laboratory *F 412 - 268 - 7129 FAX *F http://info.bio.cmu.edu/ # *U FBrf0102052 *a Riesgo-Escovar *b J. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:10:24 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 10 Mar 1998 11:10:24 +0000 *F To: juan@zool.unizh.ch *F Subject: ADRC: 032 *F Dear Juan, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'chico, a Drosophila homologue of mammalian insulin receptor substrate *F (IRS) genes, is required cell-autonomously for cell size.' *F You mention a gene that is new to FlyBase, chico. *F Do you have a map location for chico? It is nice if we can keep as many *F gene records as possible anchored to the map. Also, do you have allele *F designations for the mutants described in the abstract? *F Thank you for your help, *F with best wishes, *F Rachel. *F From juan@zool.unizh.ch Tue Mar 10 13:24:37 1998 *F Subject: Re: ADRC: 032 *F Hi Rachel: *F Indeed, chico is a new gene. I think the abbreviation will be chic, *F since any other combination is already taken. It maps to 31B, next to *F bsk. Also, we are calling the first allele we identified as chic 1, and *F it is a P element insertion; the other allele is a deficiency and already *F has a name. I hope all of this information helps. *F Sincerely: *F Dr. Juan R. Riesgo-Escovar # *U FBrf0102053 *a Omelyanchuk *b L. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:56:11 1998 *F To: ome@bionet.nsc.ru *F Subject: ADRC: 661C *F Dear Svetlana and Leonid, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'The insertion mutant 2g causing abnormal metaphases.' *F Can you tell us the symbol for your P{lArB} insertion? It is possible that *F we already have a record that includes it. Do you have a symbol/name for *F the gene defined by the 2g insertion? *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Svetlana's email address is not in FlyBase, so I'm sending it to both *F of you at Leonid's address. *F From ome@ghost.bionet.nsc.ru Tue Mar 10 14:03:22 1998 *F To: Rachel Drysdale (Genetics) *F Subject: ADRC: 661C - PlArB *F Dear Rachel, You write: *F 'The insertion mutant 2g causing abnormal metaphases.' *F Can you tell us the symbol for your P{lArB} insertion? It is *F possible that *F we already have a record that includes it. Do you have a *F symbol/name for *F the gene defined by the 2g insertion? *F Recent data show it is the insertion in the intron of cyclin B *F gene. Glad to help L Omelyanchuk # *U FBrf0102054 *a McKim *b K. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 12:04:32 1998 *F To: mckim@rci.rutgers.edu *F Subject: ADRC: 802C *F Dear Hao and Kim, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'mei-P22 encodes a novel protein required for the initiation of meiotic recombination.' *F You mention mapping two genes, mei-P22 and mei-W68 with P insertions. *F Could you tell us the symbols for the P insertions in both cases? That way *F we can capture the mapping data rigorously for FlyBase. It is nice if we can *F keep as many gene records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Hao's email address is not in FlyBase, so I'm sending it to both of you *F at Kim's address. *F From: 'Kim S. McKim' *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 802C *F Dear Rachel, *F with regards to your inquiry: *F > You mention mapping two genes, mei-P22 and mei-W68 with P insertions. *F > Could you tell us the symbols for the P insertions in both cases? *F mei-P22 is not mapped relative to any P insertions, it is itself a P *F insertion mutation mapping to 65E. We have also genetically mapped *F it between ru and h, at approximately map position 21.5. *F mei-W68 on the other hand was mapped relative to P insertion *F mutations (lethals from the genome project). The genetic order is as *F follows: *F l(2)k09810 [l(2)01103, l(2)k00705, l(2)k16914] mei-W68 l(2)k06323 *F [l(2)05338, emm]. *F The genes in brackets are not ordered relative to themselves. Some *F of this data is based on physical rather than genetic data. For *F example, the insertions 1103, k705, k16914 and k6323 are all on the *F P1 clones DS571 and DS7982. The rest of the insertions are not *F anywhere on the contig (DS00433) *F It has been reported that l(2)05338 is allelic to sm. *F Hope this covers the situation. *F Truly yours, *F Kim *F Kim S. McKim *F Waksman Institute *F Rutgers University *F 190 Frelinghuysen Rd. *F Piscataway NJ 08854-8020 *F 908 445-1164 # *U FBrf0102055 *a Benevolenskaya *b E. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:46:29 1998 *F To: mfrolov@biosci.mbp.missouri.edu *F Subject: ADRC: 406A *F Dear Elizaveta, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Identification of a novel modifier of white gene expression.' *F You describe a new gene identified as a dosage sensitive P-element *F insertion modifier of w allele expression. Do you have a gene symbol/name *F or cytological location for the new gene? Does it correspond to one of the *F genome project insertions? It is nice if we can keep as many gene records *F as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mfrolov@biosci.mbp.missouri.edu Tue Mar 10 16:19:26 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 406A *F Dear Rachel, *F My abstract 'Identification of a novel modifier of white gene expression.' *F describes a gene corresponding to a lethal P-element insertion l(2)10642. *F We named this gene Pow (Passion-of-white), since the gene is a dosage *F sensitive regulator of white gene expression. On the P1 phage map, *F l(2)10642 sequences belong to DS06061.2 clone. *F If you need any other information please don't hesitate to contact me. *F Sincerely yours, *F Elizaveta. # *U FBrf0102056 *a Andrew *b D. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:13:25 1998 *F To: debbie_andrew@qmail.bs.jhu.edu *F Subject: ADRC: 055 *F Dear Katya and Deborah, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Salivary gland formation: Regulation by SCR, EXD and the DPP signalling pathway.' *F You mention a gene that is new to FlyBase, sad. Unfortunately this symbol *F has already been taken for another gene, sad: shadow, however we can use *F Sad, in which case we should capitalise 'Sisters' in the full name. Do you *F have a map location for Sad? It is nice if we can keep as many gene *F records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Katya's email address is not in FlyBase, so I'm sending it to both of *F you at Deborah's address. *F From Debbie_Andrew@qmail.bs.jhu.edu Tue Mar 10 16:40:41 1998 *F Subject: Re: ADRC: 055 *F To: Genetics *F Reply to: RE>ADRC: 055 *F Dear Rachel, *F We would like to use the 'Sad' name and thus capitalize 'Sisters' in the full *F text. Currently the map location is 7D7-16. *F Thanks, *F Debbie # *U FBrf0102057 *a Andrew *b D. *t 1998.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 12:03:44 1998 *F To: debbie_andrew@qmail.bs.jhu.edu *F Subject: ADRC: 769C *F Dear Cristina and Deborah, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'The giant muscle protein TITIN localizes to condensed mitotic chromosomes.' *F You mention Titin, a gene that is new to FlyBase. We have a titin-like *F gene already, here is the top section from the record, with some of the *F most recent references: *F \*a bt *F \*e bent *F \*z FBgn0005666 *F \*y FBgn0005627 *F \*y FBgn0000232 *F \*y FBgn0004630 *F \*y FBgn0004518 *F \*c 102D1--102E7 *F \*c Left limit from non-inclusion within Df(4)M101-4 (FBrf0016176) *F \*c Right limit from non-inclusion within Df(4)G (citation unavailable) *F \*i Prj: Projectin *F \*i l(4)102CDa *F \*i l(4)2 *F \*i l(4)21 *F \*i l(4)23 *F \*i l(4)37 *F \*i l(4)38 *F \*d titin \like *F \*d twitchin \like *F \*d projectin *F \*J Type III fibronectin domain protein. *F \*J Immunoglobulin-C2-type-domain protein. *F \*j species == Caenorhabditis elegans; gene == unc-22 *F \*g S48824 *F \*g S49341 *F \*g M73433 *F \*g M73434 *F \*g M73435 *F \*g X66018; g8378 *F \*g L35899; g552082 *F \*g L35900; g552084 *F \*m PIR:A40985 *F \*m PIR:S24600 *F \*m SPTREMBL:Q24343 *F \*m SPTREMBL:Q24463 *F \*x FBrf0092104 == Kulp et al., 1997, A. Conf. Dros. Res. 38: 176A *F \*x FBrf0092105 == Southgate et al., 1997, A. Conf. Dros. Res. 38: 176B *F \*x FBrf0079869 == Ayme-Southgate et al., 1995, J. Cell Biol. 128(3): 393--403 *F \*x FBrf0094966 == Hochman, 1963, D. I. S. 37: 48--49 *F \*x FBrf0094967 == Hochman, 1997.7.29, personal communication *F \*x FBrf0079703 == Ziegler, 1994, Comp. Biochem. Physiol. A. 109(4): 823--833 *F \*x FBrf0057571 == Fyrberg et al., 1992, Proc. r. Soc. Biol. Sci. 249(1324): 33--40 *F \*x FBrf0088238 == Johnson and Quiocho, 1996, Nature 380(6575): 585--587 *F \*x FBrf0055012 == Ayme-Southgate et al., 1991, Proc. Natl. Acad. Sci. USA. 88: 7973--7977 *F \*x FBrf0056624 == Maroto et al., 1992, J. molec. Biol. 224: 287--291 *F \*x FBrf0084705 == Ayme-Southgate et al., 1995, Molec. Biol. Cell Suppl. 6: 150a *F \*x FBrf0084928 == Daley et al., 1995, Molec. Biol. Cell Suppl. 6: 150a *F \*x FBrf0098527 == Dickinson and Tu, 1997, Comp. Biochem. Physiol. A. Physiology 116(3): 223--238 *F \*x Lewis, Cited in Lindsley and Zimm, 1992 *F \*x FBrf0054417 == Fyrberg et al., 1991, J. Cell Sci. Suppl. 14: 27--29 *F \*x FBrf0093165 == Moore et al., 1997, Biophys. J. 72(2 Part 2): A279 *F \*x FBrf0052246 == Vigoreaux et al., 1990, J. Cell Biol. 111(5/2): 427a *F \*x FBrf0052247 == Ayme-Southgate et al., 1990, J. Cell Biol. 111(5/2): 427a *F Might this correspond to your Titin (we do not prefix symbols with D for Drosophila)? *F If not, do you have a cytological location for Titin? It is nice if we can *F keep as many gene records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Cristina's email address is not in FlyBase, so I'm sending it to both *F of you at Deborah's address. *F From Debbie_Andrew@qmail.bs.jhu.edu Tue Mar 10 16:52:05 1998 *F Subject: Re: ADRC: 769C *F Reply to: RE>ADRC: 769C *F Dear Rachel, *F Our gene is not the 'titin-like' gene encoded by the bent locus. D-Titin maps *F to 62C1,2 and has a number of characteristics suggesting that it is the *F Drosophila homologue of vertebrate muscle titin. We have a paper on our *F studies coming out soon in the Journal of Cell Biology where we have named the *F gene 'D-Titin', so we would like to keep that name if possible. There are *F other examples of D-genes in the literature. *F Thanks, *F Debbie *F P.S. D-Titin from posters in previous years has been given the names 'MCP' *F and 'non-CREST' at 62C. While those names do reflect the history of the *F isolation and characterization of the gene, they should probably be removed *F from the database. Thanks again. # *U FBrf0102058 *a Bhat *b M. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:21:16 1998 *F To: mbhat@bcm.tmc.edu *F Subject: ADRC: 126 *F Dear Manzoor, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'discs lost encodes an adherens and septate junction associated ligand of *F Neurexin IV, and is required for cell proliferation.' *F You mention a gene, dlt, that is new to FlyBase. Do you have a map *F location for this gene? It is nice if we can keep as many gene records as *F possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mbhat@bcm.tmc.edu Tue Mar 10 17:52:24 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Re: ADRC: 126 *F Dear Rachel, *F discs lost maps at 62B2-4. *F Thanks for asking. *F Manzoor # *U FBrf0102059 *a Nellesen *b D. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:50:10 1998 *F To: nellesen@biomail.ucsd.edu *F Subject: ADRC: 473B *F Dear David, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Cloning and functional characterization of the Drosophila homolog of the *F human CBF-1 Interacting Repressor (CIR).' *F You describe Cir, a gene new to FlyBase. Do you have a cytological *F location for Cir? It is nice if we can keep as many gene records anchored *F to the map as possible. Do you have symbols for the alleles of Cir that you mention? *F Could you tell us the valid symbol for the human CBF-1 Interacting *F Repressor (CIR), so that we can make a cross link to the appropriate place *F in the human databases? *F Thank you for your help, *F with best wishes, *F Rachel. *F From nellesen@biomail.ucsd.edu Tue Mar 10 17:59:29 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 473B *F Rachel, *F Glad to be of help. CIR is at 75E3. There's a P1 clone DS 02205 which *F covers the gene. I can't say yet whether any of the lesions in the area *F disrupt CIR so i'll wait on those. The human homolog is unpublished, so *F I can't say much about it yet. Thanks for your interest. *F Dave Nellesen *F nellesen@biomail.ucsd.edu # *U FBrf0102060 *a Datta *b S. *t 1998.3.10 *T personal communication to FlyBase *u *F Date: Tue, 10 Mar 1998 14:18:19 -0600 *F To: Rachel Drysdale (Genetics) *F From: sdatta@bioch.tamu.edu (Suma Datta) *F Subject: Re: ADRC: 291A *F Dear Rachel *F Since submitting the abstract it has become clear that the 640 mutation *F from the O'Brian reference is actually an allele of eve. We are writing a *F manuscript that renames 640 as 'eve5' since that seems to be the next *F number according to Flybase. Please let us know if would be the *F appropriate allele number. *F Best, Suma *F >Dear Youngji and Sumana, *F > *F > *F >I am writing in connection with your abstract for the upcoming Washington *F >DC ADRC: *F > *F >'Genetic analysis to isolate a enhancer of trol, a cell cycle activator.' *F > *F >You describe a gene, 'enhancer of troll' in 46C3--46C11. From the allele *F >designations and the O'Brien reference I know that this corresponds to the *F >'l(2)46Ce' locus in FlyBase. By what symbol are you referring to this *F >gene? The symbol that you give it ('e(trol)' would be suitable) will *F >replace the generic l(2)46Ce designation. *F > *F >Thank you for your help, *F > *F >with best wishes, *F > *F >Rachel. *F > *F >PS: Youngji's email address is not in FlyBase, so I'm sending it to both of *F >you at Sumana's address. *F > *F >---------------------------------------------------------------------- *F >Dr. Rachel Drysdale. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: rd120@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- *F Dr. Suma Datta *F Dept. Biochemistry and Biophysics *F Texas A&M University *F College Station, Tx 77843 *F 409-862-4641 Voice *F 409-845-6305 Fax *F From rd120@gen.cam.ac.uk Thu Mar 12 10:31:47 1998 *F To: sdatta@bioch.tamu.edu *F Subject: Re: ADRC: 291A *F Dear Suma, *F Thank you for the information. I will record the data about 640 under *F eve5, as you suggest, since that symbol has not been used. *F I will use eve6 for the 2751 mutation, since by implication it must also *F be an allele of eve. *F best wishes, *F Rachel. # *U FBrf0102061 *a Petitt *b M. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:39:48 1998 *F To: petitt@rascal.med.harvard.edu *F Subject: ADRC: 368C *F Hi Matt, *F I am writing in connection with your *F abstract for the upcoming Washington DC ADRC: *F 'Genes controlling cytoskeletal behavior in syncytial embryos.' *F You describe two genes that are new to FlyBase, quag and lag. Do you have *F cytological locations for them? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From petitt@rascal.med.harvard.edu Tue Mar 10 20:25:54 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 368C *F Hi Rachel- *F The gene name 'laggard' was my original name for 'fray'. It is required *F required maternally for normal mitoses in syncytial embryos. *F The gene I'm calling 'quagmire' maps to 94A8-B5 by a combination of *F deficiency mapping and meiotic mapping between P elements. It was reported *F previously as l(3)05089, which is one of the chromosomes from the Spradling *F collection of P lethals, and which is described as having a maternal effect *F in germline clonal analysis in Perrimon et al. (1996) Genetics. The P *F element maps to 91A, but itself is not associated with a lethal, even *F though it is inserted in the leader sequence of a known gene. The only *F lethal on that chromosome is the one I've mapped to 94A-B. It is very *F likely to be a mutation in the Drosophila homolog of mouse Tcp-1, although *F I haven't yet proven this. *F By the way, if quagmire and Tcp-1 turn out to be the same, do I have to go *F with the name Tcp-1? Mine would be the first mutation identified in the *F locus. It was only identified previously as a cDNA homologous to the mouse *F gene. *F That's about it. *F Best regards, *F Matt # *U FBrf0102062 *a Cline *b T. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 12:00:14 1998 *F To: sxlcline@uclink.berkeley.edu *F Subject: ADRC: 717A *F Dear Bryan and Tom, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'A novel Sex-lethal germline promoter?' *F You mention a new Sxl allele, SxlfPS1, and say *F Most useful was SxlfPS1, a 4.5 kb intragenic deletion generated by *F mobilization of a P-element in exon 2. Could you tell me the progenitor *F strain for SxlfPS1 so that I can record its provenance accurately? *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Bryan's email address is not in FlyBase, so I'm sending it to both of *F you at Tom's address. *F From sxlcline@uclink.berkeley.edu Tue Mar 10 23:07:16 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 717A *F RE *F >Most useful was SxlfPS1, a 4.5 kb intragenic deletion generated by *F >mobilization of a P-element in exon 2. Could you tell me the progenitor *F >strain for SxlfPS1 so that I can record its provenance accurately? *F Dear Rachel, *F Well, let's see. I'll have to make up a name. Let's call it SxlM1,fPw+c. *F That is a PlacW insertion into exon 2 that is a null. But perhaps I should *F point out that both SxlfPS1 and SxlM1,fPw+c are on a SxlM1 background *F (that's a gof 9.5 kb roo insertion between exons 3 and 4, hence it is still *F present in its original form in fPS1). *F Regards, *F Tom Cline # *U FBrf0102063 *a Smolik *b S. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 12:05:45 1998 *F To: smoliks@ohsu.edu *F Subject: ADRC: 819B *F Dear Brenda and Sarah, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Cloning and characterization of the D. melanogaster homologue of SIR2' *F You describe Sir2, a gene new to FlyBase. Do you have a cytological *F location for Sir2? It is nice if we can keep as many gene records anchored to *F the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Brenda's email address is not in FlyBase, so I'm sending it to both of *F you at Sarah's address. *F From smoliks@ohsu.edu Wed Mar 11 01:26:30 1998 *F To: rd120@gen.cam.ac.uk *F Subject: ADRC: 819B -Reply *F Hi Rachel, *F Good to hear from you .... you guys don't waste any time do you?! The SIR2 *F homologue maps to position 34A on the second chromosome. I suppose I should *F come up with a proper name before M. Ashburner re-names it ...yes? *F Sarah # *U FBrf0102064 *a Lu *b X. *t 1998.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:02:31 1998 *F To: Xlu@kuhub.cc.ukans.edu *F Subject: ADRC: 028 and 540A *F Dear Xiangyi, *F I am writing in connection with your abstracts for the upcoming Washington *F DC ADRC: *F 'A suppressor of Draf encodes Dsrc42A which shows unusual negative *F signaling role in the receptor tyrosine kinase (RTK) pathway.' *F and *F 'semang is required for the development of R1, R6, R7 and cone cells in the *F eye.' *F Concerning your 'Dsrc42A': FlyBase knows of a nearby gene called Src41. *F Here is an extract from FlyBase about this gene. *F \*a Src41 *F \*z FBgn0004603 *F \*y FBgn0010284 *F \*b 2- *F \*c 41B1--41C7 *F \*c Left limit from in situ hybridisation (FBrf0089086) *F \*c Right limit from in situ hybridisation (FBrf0089086) *F \*i Tk5: Tyrosine kinase 5 *F \*i Dsrc41 *F \*i Dtk5 *F \*F protein-tyrosine kinase == EC 2.7.1.112 *F \*j species == Mus; gene == Src; MGI:98397 *F \*j species == Homo sapiens; gene == SRC; GDB:120750; OMIM:190090 *F \*g D42125; g1536790 *F \*g S55977; g236022 *F \*m PIR:S18013 *F \*m SPTREMBL:Q26297 *F \*m SPTREMBL:Q94879 *F \*x FBrf0053997 == Shishido et al., 1991, FEBS Lett. 289: 235--238 *F \*x FBrf0089086 == Takahashi et al., 1996, Genes Dev. 10(13): 1645--1656 *F Is this gene the same as your 'Dsrc42A'? I realise that the cytological *F locations are slightly different, but since they are so close we felt we *F should check. *F Also, you mention another new gene, semang. Do you have a map location for *F semang? It is nice if we can keep as many gene records as *F possible anchored to the map. *F Do you have allele designations for the mutants of 'Dsrc42A' or semang that *F you describe? *F Thank you for your help, *F with best wishes, *F Rachel. *F From xlu@KUHUB.CC.UKANS.EDU Wed Mar 11 02:15:43 1998 *F Subject: Re: ADRC: 028 and 540A-reply *F To: rd120@gen.cam.ac.uk *F Dear Rachel at FlyBase: *F Dsrc42A cDNA differs with the published Dsrc41 by only one amino acid *F residue. Southern analysis of overlapping deficiency chromosomes suggest that *F there is only one Dsrc42A-hybridizing signal on 2R. Therefore, Dsrc42A=Dsrc41. *F Mutation in Dsrc42A is recessively lethal. Dsrc42A/Deficiency is also lethal. *F This allowed us to localize Dsrc42A between two deficiency chromosomes, *F Df(2R)nap9 and Df(2R)bwvDe2LCy2R. Thus, the cytological location of Dsrc42A is *F 42A1-2 to 42A2-4. We have many alleles which are included in a manuscript *F submitted recently. semang (sag) maps to polytene 54. We have not found a *F deficiency uncovering semang. You ask for allele designations, I am not sure *F what information do you want? *F Xiangyi Lu *F University of Kansas *F Lawrence, KS 66045 # *U FBrf0102065 *a Okano *b H. *c K. *d Tabuchi *t 1998.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:51:32 1998 *F To: okano@nana.med.osaka-u.ac.jp *F Subject: ADRC: 545C *F Dear Katsuhiko and Hideyuki, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Drosophila paired-like homeobox gene expressed in the subsets of *F developing neurons and epidermis.' *F You describe unc-4, a gene new to FlyBase. Do you have a cytological *F location for unc-4? It is nice if we can keep as many gene records anchored *F to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F PS: Katsuhiko's email address is not in FlyBase, so I'm sending it to both *F of you at Hideyuki's address. *F From okano@nana.med.osaka-u.ac.jp Wed Mar 11 02:24:10 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 545C *F Dear Rachel, *F Thank you for your e-mail. *F We are happy to announce the cytological location on the FlyBase, as a new g *F ene. *F The cytological location for Drosophila unc-4 is 16D1-2. *F Yours sincerely, *F Hideyuki Okano and Katsuhiko Tabuchi *F \**************************** *F Hideyuki Okano, M.D., Ph.D. *F Professor *F Department of Neuroanatomy *F Biomedical Research Center *F Osaka University Medical School *F 2-2 Yamadaoka, Suita, Osaka 565 Japan *F Phone 81-6-879-3581 *F FAX 81-6-879-3589 # *U FBrf0102066 *a Cribbs *b D. *t 1998.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:25:41 1998 *F To: bourbon@cict.fr *F Subject: ADRC: 171C *F Dear Henri-Marc, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'poils aux pattes (hairy legs), an evolutionarily conserved modifier of *F proboscipedia homeotic cell identity function' *F You discuss, but do not name, alleles of pap. Do you have symbols for *F these alleles? Also, do you have a map location for this *F gene? It is nice if we can keep as many gene records as possible anchored *F to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From cribbs@cict.fr Wed Mar 11 10:40:53 1998 *F To: rd120@gen.cam.ac.uk *F Subject: your enquiry to Henri Bourbon *F Dear Dr. Drysdale, *F In response to your query to Henri Bourbon concerning poils aux pattes (pap): *F pap is a previously undescribed gene isolated as a P insertion mutation. *F The original insertion is named papEP1. All other alleles were by *F imprecise excision, and we have quite a few of those. The original allele *F behaves as a null allele or nearly (provided that those excisions that *F delete the putative ATG delete the true ATG and represent true nulls). I am *F not sure the names of these alleles will be helpful, but please tell me if *F I am wrong... *F The cytological localization of the P insertion on polytenes is at 78A. The *F low recombination frequency with Ki supports this localization. *F Don't hesitate to let me know if you need additional information. *F With best regards, *F David # *U FBrf0102067 *a Chen *b J. *t 1998.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:58:03 1998 *F To: Laski@ewald.mbi.ucla.edu *F Subject: ADRC: 691C *F Dear Jiong and Frank, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Analysis of no terminal filament, a gene involved in ovarian morphogenesis.' *F You write about a new gene, ntf. Do you have a cytological location for *F ntf? It is nice if we can keep as many gene records anchored to the map as *F possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jchen@mbi.ucla.edu Thu Mar 12 23:13:01 1998 *F To: rd120@gen.cam.ac.uk *F Subject: ntf location *F Dear Dr. Drysdale, *F The ntf mutant was isolated from a P-element mutagenesis screen, but *F unfortunately the mutation was not caused by P-element insertion. I am *F currently mapping this gene, the approximate location is 60A-D. *F Sincerely yours, *F Jiong Chen # *U FBrf0102068 *a Lengyel *b J. *t 1998.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:59:22 1998 *F To: jlengyel@ucla.edu *F Subject: ADRC: 694C *F Hi Judith, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Genetic control of hindgut morphogenesis.' *F You write about a new gene, drumstick. Do you have a short symbol for *F drumstick? If not we will just use the whole name until you do. Do you *F have a cytological location for drumstick? It is nice if we can keep as *F many gene records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jlengyel@protos.lifesci.ucla.edu Wed Mar 11 22:41:58 1998 *F Subject: Re: ADRC: 694C *F To: 'Rachel Drysdale' *F abbreviation is 'drm'--this should not be redundant with any known gene. *F Map position at moment, crude, is 23DEF/24A *F Dr. Judith A. Lengyel # *U FBrf0102069 *a Jeong *b S.M. *t 1998.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 12:05:14 1998 *F To: njuni@fly.erato.jst.go.jp *F Subject: ADRC: 815A *F Dear Sang-Min and Naoto, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Drosophila melanogaster DQ1 is homologous to recQ gene family.' *F (Naoto - You were the only author for whom I could find an email address. *F Feel free to pass this query on to the most appropriate author, if it is *F not best for you to answer it.) *F FlyBase already knows of one RecQ, for which the gene is called gesta1. *F Here is the gene record for gesta1: *F \*a gesta1 *F \*z FBgn0015800 *F \*x FBrf0085400 == Kusano et al., 1996, A. Conf. Dros. Res. 37: 152 *F \*x FBrf0091897 == Kusano et al., 1997, A. Conf. Dros. Res. 38: 106C *F \*E FBrf0091897 == Kusano et al., 1997, A. Conf. Dros. Res. 38: 106C *F \*e genome stabilizer 1 *F \*i RecQ: RecQ-like *F \*F helicase *F \*j species == Escherichia coli; gene == recQ; CGSC:17959; ECOGENE:EG10833 *F \*j species == Homo sapiens; gene == BLM; GDB:135698; OMIM:210900 *F \*j species == Saccharomyces cerevisiae; gene == SGS1; SGDID:L0001877 *F \*E FBrf0085400 == Kusano et al., 1996, A. Conf. Dros. Res. 37: 152 *F \*i RecQ: RecQ-like *F Does this correspond to your Q1? *F If not, do you have a cytological location for Q1? It is nice if we can *F keep as many gene records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From: jeong@louise.riken.go.jp *F To: rd120@gen.cam.ac.uk *F Subject: reply for question of ADRC-815A *F Dear Dr. Rachel Drysdale, *F I have received the e-mail from Naoto Juni. *F DQ1 gene was located on chromosome 3L-70DE. *F We have examined Dq1 protein in vivo by immunofluoresence-modified anti-Dq1 *F antibody. *F We could not get the best result. Now we are planning the more detail *F experiment. *F It was estimated that Dq1 protein localize into the nucleus. *F (Dq1 has the putative NLS in amino terminal.) *F Thank you for your request, *F with best wishes, *F Jeong. # *U FBrf0102070 *a Botas *b J. *t 1998.3.13 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:24:45 1998 *F To: jbotas@bcm.tmc.edu *F Subject: ADRC: 161B *F Dear Cheng-Hsin and Juan, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'DLDB: a protein that interacts with AP LIM domains and is required for *F dorsoventral compartmentalization in the wing' *F You mention a gene, Ldb, that is new to FlyBase. Do you have a full name *F or a map location for this gene? It is nice if we can keep as many gene *F records as possible anchored to the map. You also mention mutants -- do *F you have symbols for these yet? *F Thank you for your help, *F with best wishes, *F Rachel. *F From jbotas@bcm.tmc.edu Fri Mar 13 05:07:48 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 161B *F Hi Rachel, *F The map location is 60A. As for the definitive name we are waiting to do *F some complementation tests with alleles of a gene called Chip. Based on map *F location and sequence, it seems likely that Ldb is the same as Chip. *F However we have been unable, so far, to obtain Chip alleles from the people *F working on this gene. *F Cheers *F Juan Botas # *U FBrf0102071 *a Prokopenko *b S. *c H. *d Bellen *t 1998.3.11 *T personal communication to FlyBase *u *F >From sp691305@bcm.tmc.edu Wed Mar 11 17:06:06 1998 *F Subject: update for FlyBase: mm = mbl *F To: ag24@gen.cam.ac.uk *F Dear Aubrey, *F I have another update for FlyBase. In the course of doing my molecular *F screen I have found that mindmelt (mm) (Kania et al., 1995) is allelic to *F muscleblind (mbl) (Begemann et al., 1997). *F I have plasmid rescued the genomic fragment flanking the 71/3 P element *F allele of mindmelt using EcoRI and sequenced both ends of the rescued *F fragment. The sequence of the proximal end of this fragment showed 98% *F identity (over 518 nt.) to the published sequence of the mbl cDNA *F (Begemann et al., 1997). *F In addition, the 71/3 allele of mm fails to partially complement (9/158) *F the strong mblE27 allele (P element excision which affects the coding *F region of the gene and is presumably a null allele (Begemann et al., *F 1997)). Escapers had wing blisters and wing venation defects; two flies *F had unexpanded wings. *F The 71/3 allele of mm complemented two weak alleles of mbl - mbl05507 (P *F element insertion allele) (26/97) and mblE127 (P element excision which *F does not affect the coding region of the gene) (42/155). *F Finally, the 71/3 mindmelt insertion was mapped by in situ hybridization *F to 54A (Kania et al., 1995) and the muscleblind gene was mapped to 54A1-3 *F (Begemann et al., 1997). *F Therefore, I concluded that mm is allelic to mbl. *F This information, when included in FlyBase, should be referred to as *F S.Prokopenko and H.J.Bellen, personal communication. *F Feel free to contact me at sp691305@bcm.tmc.edu, if you have any questions *F or need more detailed information. *F Best wishes, *F Sergei *F Sergei Prokopenko *F Bellen lab # *U FBrf0102072 *a Chen *b B. *c T. *d Chu *t 1998.3.13 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 13 11:47:40 1998 *F To: sid@pharm.sunysb.edu *F Subject: ADRC: 143 *F Dear Bin and Sidney, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'High resolution mapping of Drosophila mutations using site-specific male *F recombination.' *F John Roote mentioned to me that some of the mapping data for cortex that is *F presented in your abstract is in error. Please could you confirm that for *F me, and I will make sure we exclude anything that is potentially *F confusing. *F Many thanks for your help, *F Rachel. *F From chu@pharm.sunysb.edu Fri Mar 13 15:25:01 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 143 *F CC: sid@pharm.sunysb.edu, chenb@pharm.sunysb.edu *F Dear Dr. Drysdale: *F Cortex is now placed distal to black and distal to the 34A region, *F based on male recombination mapping utilizing additional P elements. *F Sincerely, *F Bin and Tehyen # *U FBrf0102073 *a Johnson *b R. *t 1998.3.13 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:42:43 1998 *F To: rjohnson@cmgm.stanford.edu *F Subject: ADRC: 375A *F Dear Ron, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Identification of a Drosophila homolog of NPC1- a protein related to Patched.' *F You describe 'NPC1', a gene that is new to FlyBase. Do you have a full *F name for this gene, or a cytological location for NPC1? It is nice if we *F can keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rjohnson@cmgm.stanford.edu Fri Mar 13 20:12:51 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 375A *F Dear Rachel, *F Thanks for your message. NPC1 stands for Niemann-Pick Type C1 disease *F which is a human disorder that involves progressive neurodegeneration and *F hepatosplenomegaly. It is caused by a cellular defect in cholesterol *F homeostasis. In our unpublished work, we have identified a fly EST which is *F related to the human and mouse NPC1 sequences. The EST appears to be full *F length for NPC1 and is chimeric (containing seq for ribosomal RNA and other *F sequences). The NPC1 sequence maps to 31B1-5 by in situ. The entire EST *F maps to the same place- even though its chimeric, 80% of the sequence codes *F for NPC1. Right now we are calling it NPC1 but that name my change *F depending on the mutant phenotype which we are still pursuing *F Sincerely, *F Ron Johnson, Ph.D. *F Laboratory of Matthew P. Scott *F Department of Developmental Biology *F B371, Beckman Center *F 279 Campus Drive *F Stanford University Medical Center *F Stanford, CA 94305-5329 *F phone (650) 725-7622 *F fax (650) 723-9878 # *U FBrf0102074 *a Russell *b S. *t 1998.3.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:24:12 1998 *F To: sr120@gen.cam.ac.uk *F Subject: ADRC: 149B *F Hi Steve and Samantha, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Molecular and Genetic Characterisation of Drosophila Sox Genes *F ' *F You mention two genes, SoxB and SoxE, that are new to FlyBase. Do you have *F a map location for these gene? It is nice if we can keep as many gene *F records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From sr120@mole.bio.cam.ac.uk Mon Mar 16 16:18:17 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 149B *F SoxB is at 100B and SoxE is at 50D/E (we are not sure if it is at the very *F end of D or the start of E) *F Steve # *U FBrf0102075 *a Subramaniam *b V. *t 1998.3.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:49:20 1998 *F To: vsubramaniam@bmg.bhs.uab.edu *F Subject: ADRC: 445A *F Dear Vaidyanathan, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'A novel gene that regulates Sex-lethal expression and Dosage Compensation.' *F You describe a mutation, fl338, defining a gene new to FlyBase. Is this *F the symbol by which you wish the gene to be known? What symbol should we *F use to denote the allele, fl3381, or fl338338? Do you have a *F cytological location for fl338? It is nice if we can keep as many gene *F records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From VSUBRAMANIAM@bmg.bhs.uab.edu Mon Mar 16 17:37:11 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Re: ADRC: 445A *F Dear Rachel, *F For the nonce, I would like the gene to be known as fl338. (When we know *F more about it, we might christen it appropriately). Also, fl3381 sounds *F like a good designation for the allele. I have mapped this mutation *F genetically to 1- 0.6 and cytologically to the 2B9-2C1 interval. *F If there is anything else I can help with, please do not hesitate to *F contact me. *F regards, *F vaidya. # *U FBrf0102076 *a Brill *b J. *c C. *d Wood *e R. *f Farkas *g M. *h Fuller *t 1998.3.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:18:45 1998 *F To: brill@cmgm.stanford.edu *F Subject: ADRC: 105 *F Dear Julie, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Ring canal morphogenesis in the male germ line.' *F You mention a gene, 'separation-anxiety', that is new to FlyBase. Do you *F have a symbol or a map location for this gene? It is nice if we can keep *F as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From brill@u.washington.edu Mon Mar 16 17:49:25 1998 *F To: Rachel Drysdale *F cc: Minx Fuller , *F Cricket Wood *F Subject: Re: ADRC: 105 *F Hi Rachel, *F separation anxiety (abbreviation yet to be determined - suggestions *F appreciated) is a new mutation identified from a screen for male sterile *F mutations conducted by Barbara Wakimoto et al. (see abstract number 106). *F I do not know the map position (you could get that information from Minx), *F but the mutation is located on chromosome 2. *F There is another gene (also from Barbara Wakimoto's male sterile *F screen), provisionally called 'four way stop' (abbrev. fws) that has a *F similar phenotype and has also been characterized and mapped by Rebecca *F and Cricket. It is also on chromosome 2 and is uncovered by a deficiency. *F There is one allele of separation anxiety and there are two alleles of *F fws. The phenotype for mutations in both of these genes is a failure in *F meiotic cytokinesis ('four wheel drive' phenotype), as well as a failure *F in proper polarization and bundling of the cysts of elongating spermatids. *F The end result is an accumulation of ovoid cysts containing multinucleate *F spermatids. The flies are male sterile. *F Hope this helps. *F Sincerely, *F Julie Brill *F P.S. I am forwarding a copy of this to Minx and also to Cricket in case *F either of them has time to provide additional information. *F From wood@cmgm.stanford.edu Thu Mar 19 03:23:11 1998 *F Subject: fwd like mutants *F To: rd120@gen.cam.ac.uk, brill@u.washington.edu, fuller@cmgm.Stanford.EDU *F Hi Minx, Julie and Rachel, *F I was away when you sent the emails about the fwd mutants. Here is the *F latest mapping information on them. *F 12-41 'separation anxiety' maps between al and dp by recombination and to *F 22D5-E1; 22F2 or 23C or 23E3-6;24A3-4 by deficiency *F Hope all this information is helpful! *F Cricket Wood *F Fuller Lab RA # *U FBrf0102077 *a Cho *b N. *t 1998.3.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:58:46 1998 *F To: cho@saturn.med.nyu.edu *F Subject: ADRC: 693B *F Dear Nam, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'waldo affects an early step in primordial germ cell migration.' *F You write about a new gene, wdo. Do you have a cytological location for *F wdo? It is nice if we can keep as many gene records anchored to the map as *F possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From cho@saturn.med.nyu.edu Mon Mar 16 18:13:08 1998 *F To: Rachel Drysdale *F Subject: Re: ADRC: 693B *F Dear Rachel, *F Thank you for your email regarding waldo. I am afraid the only definitive *F information regarding wdo's cytological location that I have is that it is *F located on 2L. I am currently mapping wdo. I will be sure to let you *F know once I have better information. *F Take care, Nam Cho # *U FBrf0102078 *a Amanai *b K. *t 1998.3.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:43:17 1998 *F To: kazuhito@jhuvms.hcf.jhu.edu *F Subject: ADRC: 379B *F Dear Kazuhito, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Molecular and genetic analysis of a Drosophila ubiquitin protein ligase *F encoded by hyperplastic discs' *F You describe 'Tabp', a gene that is new to FlyBase. Do you have a *F cytological location for Tabp? It is nice if we *F can keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kazuhito@JHUVMS.HCF.JHU.EDU Mon Mar 16 19:28:23 1998 *F Subject: Re: ADRC: 379B *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F We are not really sure about cytological location for 'Tabp' at this point, *F but chromosome in situ experiment suggests it's 85D-F. *F Sincerely yours, *F Kazuhito # *U FBrf0102079 *a Xie *b T. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:18:15 1998 *F To: xie@mail1.ciwemb.edu *F Subject: ADRC: 100 *F Dear Ting, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Communication between Soma and Germ Cells Is Critical for the Maintenance *F and Proliferation of Germline Cells in the Germarium.' *F You mention a gene, eel, that is new to FlyBase. Do you have a map *F location for this gene? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From xie@mail1.ciwemb.edu Tue Mar 17 07:44:48 1998 *F Subject: Re: ADRC- 100 *F To: 'Genetics' *F Reply to: RE>ADRC: 100 *F Sorry for the delay. It is located in 63D. *F Ting Xie # *U FBrf0102080 *a Hoang *b R. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:45:35 1998 *F To: rehoang@princeton.edu *F Subject: ADRC: 399A *F Hi Rachel, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'The control of cell shape changes during ventral furrow formation.' *F You describe 'Accordion', a gene that is new to FlyBase. Do you have a *F symbol or a cytological location for Accordion? It is nice if we can keep *F as many gene records as possible anchored to the map. *F Thank you for your help, *F all the best, *F Rachel. *F From rehoang@Princeton.EDU Tue Mar 17 13:50:13 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 399A *F Hi Rachel, *F I'm happy to supply the map information we have on Accordion (Aco). By deficiency mapping it's located *F in 24F1-4, though nailing it to a known locus in this region has been a *F little confusing so far. I'll keep you posted! *F Rachel. # *U FBrf0102081 *a Roote *b J. *t 1998.3.17 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Mar 17 13:58:09 1998 *F To: rd120@mole.bio.cam.ac.uk *F Dear Rachel, *F A few Adh region amendments/additions. *F 1) Df(2L)b81f2 is not the same as b81f2 (b allele on CyO). Call the *F deletion b81f2A? *F 2) T(2;3)b83l2 is Df(2L) 34Db -- 34Fa not 34Db -- 34Dg *F 3) Df(2L)TE35D-24 is wb-fs(2)35Ec not elA-fs(2)35Ec *F 4) new entry: *F In(2L)el94 *F Cytology: In(2L)35B;;36D *F base ch: dp fy2 pr sple *F spontaneous *F genetics: elB--elA- # *U FBrf0102082 *a Certel *b K. *t 1998.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:49:39 1998 *F To: kcertel@blue.weeg.uiowa.edu *F Subject: ADRC: 447C *F Dear Kaan, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Genetic dissection of POU-domain function: Characterization of second site *F modifiers of Ventral veinless/Drifter'. *F You describe En(vvl) (allele En(vvl)5), a gene new to FlyBase. Do you *F have a cytological location for En(vvl)? It is nice if we can keep as many *F gene records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kaan-certel@uiowa.edu Mon Mar 16 17:07:02 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 447C *F Hi Rachel, *F In the abstract, I talked about mutants En(vvl)5 and En(vvl)21 which are *F allelic to one another. Turns out En(vvl)5 and En(vvl)21 are alleles of Mad *F (Mothers against dpp). So, for at least now, this is the only information I *F can confidently give you about these mutants. *F Please contact me if you have any additional questions. *F Kaan *F From rd120@gen.cam.ac.uk Tue Mar 17 09:33:50 1998 *F To: kaan-certel@uiowa.edu *F Subject: Re: ADRC: 447C *F Hi Kaan, *F Many thanks for getting back to me. *F You abstract only discusses En(vvl)5 by name. But I will curate your *F message about En(vvl)5 and En(vvl)21 as a personal communication to FlyBase *F from you, to capture their allelism with Mad. *F Unfortunately there is already a Mad5... What would you like the symbols *F of these two new Mad alleles to be? You could go with MadE5 and *F MadE21, how about that? The fact that they enhance vvl alleles will be *F captured in the information about the mutants, and we need not try to *F encode that in the symbol. *F Looking forward to hearing from you, *F Rachel. *F From kaan-certel@uiowa.edu Tue Mar 17 16:26:17 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 447C *F Hi Rachel, *F >Unfortunately there is already a Mad5... What would you like the symbols *F >of these two new Mad alleles to be? You could go with MadE5 and *F >MadE21, how about that? The fact that they enhance vvl alleles will be *F >captured in the information about the mutants, and we need not try to *F >encode that in the symbol. *F Sounds great to me. As long as we don't confuse people with the symbols, *F they won't be Mad at us (I love this gene, there is no end to puns with the *F name! Makes great talk titles, too.). I like your idea for the symbols, it *F should work great. Thanks for the message. *F Kaan # *U FBrf0102083 *a Shih *b H. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:40:43 1998 *F To: peifer@unc.edu *F Subject: ADRC: 371C *F MARK FORWARDED MAIL to hshih@email.unc.edu *F Hi Mark, *F I am writing to you because neither Hsin-Pei nor John has an email address *F in FlyBase, but feel free pass the query on to whoever is most appropriate. *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Novel putative protein partners of Drosophila septins identified in a two- *F hybrid screen' *F You describe two genes 'sip1' and 'sip2', both new to FlyBase. *F Do you have a cytological location for either of them? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From hshih@email.unc.edu Tue Mar 17 18:49:22 1998 *F To: rd120@gen.cam.ac.uk *F Subject: cytological position of sip1 and sip2 *F Hi *F sip1-25F1/F2 *F sip2-66B5 or/and 66B11 *F these data are based on the P1 blot screening and PCR checking. *F Hsin # *U FBrf0102084 *a Rushlow *b C. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:28:51 1998 *F To: roth@gen.mpib-tuebingen.mpg.de, rushlow@is.nyu.edu *F Subject: ADRC: 244A and 245B *F Dear Anna, Christine and Siegfried, *F I am writing in connection with your abstracts for the upcoming Washington *F DC ADRC: *F 'brk, a component of the dpp pathway, affects patterning of the Drosophila *F appendages' *F and *F 'brinker, a negative regulator of the dpp pathway' *F You describe a gene, brk, that is new to FlyBase. Do you *F have a map location for brk? It is nice if we can keep as many gene *F records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rushlow@is.nyu.edu Tue Mar 17 19:50:50 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 244A and 245B *F brinker maps to the X chromosome at 19.6 # *U FBrf0102085 *a Belote *b J. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:46:02 1998 *F To: jbelote@mailbox.syr.edu *F Subject: ADRC: 404B *F Dear Kerrie-Ann and John, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Positional cloning of DTS7, encoding a proteasome beta-type subunit.' *F You describe 'Su(DTS)-1', a gene that is new to FlyBase. Do you have a *F symbol or a cytological location for Su(DTS)-1? It is nice if we can keep *F as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jbelote@mailbox.syr.edu Tue Mar 17 20:58:40 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 404B *F Dear Rachel, *F Su(DTS)-1: this is a dominant suppressor of the dominant *F temperature-sensitive lethal mutants, DTS5 (also known as Pros26 or *F l(3)73Ai1) and DTS7. These DTS mutants both encode altered proteasome *F subunits. We are currently trying to characterize this gene more *F thoroughly, and we don't have a good cytological position of it yet, but by *F recombination mapping it maps at 3-48.5, just to the right of pink. *F I hope this info suffices. *F John # *U FBrf0102086 *a Tanda *b S. *t 1998.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:29:20 1998 *F To: siali@wam.umd.edu *F Subject: ADRC: 254B *F Dear Syed, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Mutations in the obake gene lead to significant head deformities.' *F You describe a gene, obake, that is new to FlyBase. Do you have a map *F location for obake? It is nice if we can keep as many gene records as *F possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rd120@gen.cam.ac.uk Tue Mar 10 11:29:46 1998 *F To: epps@zool.umd.edu *F Subject: ADRC: 255C *F Dear Janet, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'hunchback-like (hbl) encodes a ubiquitin conjugating enzyme and *F potentially functions as a Gap gene.' *F You describe a gene, hbl, that is new to FlyBase. Do you have a map *F location for hbl? It is nice if we can keep as many gene records as *F possible anchored to the map. You also describe an hbl mutant allele *F in some detail (the PZ insertion). Do you have a symbol for this allele? *F Thank you for your help, *F with best wishes, *F Rachel. *F From rd120@gen.cam.ac.uk Tue Mar 10 11:30:10 1998 *F To: tanda@zool.umd.edu *F Subject: ADRC: 256A *F Dear Soichi, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'oroshigane (oro), a segment polarity gene, encodes a multiple pass *F transmembrane protein with conserved phosphorylation sites for both Protein *F Kinase A and Casein Kinase II.' *F You wrote: *F 'This protein has 52\% identity to the 52.8 KD hypothetical protein *F isolated from C. elegans, and 33\% identity to the 67 KD hypothetical *F protein isolated from Yeast.' *F We would be most grateful if you could tell us the names of the worm and *F yeast genes. *F Thank you for your help, *F with best wishes, *F Rachel. *F From TANDA@zool.umd.edu Tue Mar 17 21:12:26 1998 *F To: rd120@gen.cam.ac.uk *F Subject: ADRC: 254B, 255C, 256A *F Dear Rachel, *F I am responsible for three memo that you sent to my lab. *F 254B, obake mutation. *F We mapped this mutation in 48B2;48B7 based on our complementation tests *F using Df(2R)en spx31, Df(2R)enA and Df(2R)enB. Obake is not allelic to *F en based on our complementation tests using en1 and Df(2R)enE. *F 255C, hunchback-like. We renamed it to semushi. Its symbol is semi. *F Location:21C6-7. This is l(2)02858 in FlyBase. Bloomington stock# is *F P1214. The deduced semushi protein shows the highest homology with *F human ubiquitin-conjugated enzyme E2 (acc#P50550). *F 256A, oroshigane, *F They do not have names yet. They are the sons/daughters of genome *F projects. They are listed as hypothetical proteins. *F The C. elegans one is hypothetical 52.8 KD protein (Acc#; P49049) *F The yeast one is hypothetical 67.5 KD protein (Acc#;P34248) *F If you need more, please let me know. *F Best wishes. *F Soichi *F From rd120@gen.cam.ac.uk Wed Mar 18 13:06:23 1998 *F To: TANDA@zool.umd.edu *F Subject: Re: ADRC: 254B, 255C, 256A *F Dear Soichi, *F I will curate your mail as a personal communication to FlyBase, as the *F source of the map data etc. *F 254B: obake. Just checking the details -- I think the results will have *F been that Df(2R)en-A and Df(2R)en-B complement (do not delete) obake, and *F that Df(2R)en-SFX31 (which I presume corresponds to your 'Df(2R)en spx31') *F fails to complement (does delete) obake. Please could you confirm this for *F me? *F 255C: The 'hbl' allele will have the symbol semi02858 *F Very best wishes, *F Rachel. *F From TANDA@zool.umd.edu Wed Mar 18 14:42:26 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Re: ADRC: 254B, 255C, 256A *F Rachel, *F What you wrote is fine, but I would like to make sure that you change *F the name of semi02858 from hunchback-like to semushi (semushi means *F hunchback in Japanese). *F Thanks, *F Soichi *F From rd120@gen.cam.ac.uk Wed Mar 18 14:46:18 1998 *F To: TANDA@zool.umd.edu *F Subject: Re: ADRC: 254B, 255C, 256A *F Hi Soichi, *F >change *F >the name of semi02858 from hunchback-like to semushi *F Indeed, semi will be the valid symbol for the gene referred to as 'hbl: *F hunchback-like' in your abstract. *F >semushi means *F >hunchback in Japanese *F thank you for this little bit extra, I will add it to the data. *F Rachel. # *U FBrf0102087 *a Dobens *b L. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:11:21 1998 *F To: leonard_dobens@cbrc.mgh.harvard.edu *F Subject: ADRC: 034 *F Dear Leonard, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Dpp receptors Sax and Tkv have differential effects on follicle cell gene *F expression.'. *F You mention a gene that is new to FlyBase, A359. We were wondering you *F have a more descriptive symbol/name for this gene by now? Also, do you *F have a map location for A359? It is nice if we can keep as many gene *F records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From leonard_dobens@cbrc.mgh.harvard.edu Wed Mar 18 07:56:24 1998 *F Subject: RE>ADRC- 034 *F To: Rachel DrysdaleGenetics *F RE>ADRC: 034 03/17/98 10:12 AM *F Dear Rachel, *F 'A359' is our shorthand for A359.1M3 a P element enhancer trap from the Gehring screen. *F I think the insertion is not new to Flybase *F Hugo Bellen mapped the insertion to 68C, I believe, but please let me check that location and get back to you on it. *F All the best, *F Len *F From rd120@gen.cam.ac.uk Wed Mar 18 13:42:32 1998 *F To: leonard_dobens@cbrc.mgh.harvard.edu *F Subject: Re: RE>ADRC- 034 *F Hi Len, *F >'A359' is our shorthand for A359.1M3 a P element enhancer trap from the Gehring screen. *F >I think the insertion is not new to Flybase *F Odd though it seems, it is new to FlyBase. Can you confirm whether (or *F not!) it is a P{lArB} insertion? I'll curate your message as a personal *F communication to FlyBase, as a source for the mapping data. *F If you know of any publications where it has appeared then please could you *F send me the references. If we've missed it somewhere then I should go and *F find it. *F best wishes, *F Rachel. # *U FBrf0102088 *a Jarman *b A. *t 1998.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:51:06 1998 *F To: andrew.jarman@ed.ac.uk *F Subject: ADRC: 534A *F Dear Sarah and Andrew, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'New atonal-related genes in D. melanogaster.' *F You describe Ath-B, a gene new to FlyBase. Do you have a cytological *F location for Ath-B? It is nice if we can keep as many gene records anchored *F to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Andrew.Jarman@ed.ac.uk Wed Mar 18 12:48:26 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 534A *F Dear Rachel, *F Concerning our reference to a new gene, Dath-B in our abstract. We have *F located this gene to 53A. Also, we have decided to name this gene 'cousin *F of atonal' (cato). *F Best wishes *F Andy # *U FBrf0102089 *a McKearin *b D. *t 1998.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 12:02:21 1998 *F To: leon@utsw.swmed.edu, mckearin@utsw.swmed.edu *F Subject: ADRC: 736B 737C *F Dear Chitra, Arlene and Dennis, *F I am writing in connection with your abstracts for the upcoming Washington *F DC ADRC: *F 'Characterization of a novel Bam interacting protein, BITP 15.' *F 'Studies of Bam propose a role for organelle vesicle fusion in cystoblast differentiation.' *F You describe three new genes BITP-15, Tera and Plap. Do you have full *F names for any of these gene symbols? Do you have a cytological location for any *F of them? It is nice if we can keep as many gene records anchored to the map *F as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mckearin@utsw.swmed.edu Wed Mar 18 14:20:57 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 736B 737C *F Dear Rachel, *F dPLAP is the fly homolog of Phospholipase A2 Activator Protein. The gene is *F located at 22A. We don't have any mutations as yet so we don't have a *F phenotype-based name. *F Hope this helps. *F Regards, Dennis *F From mckearin@utsw.swmed.edu Wed Mar 18 14:54:06 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 736B 737C *F Rachel, *F If you're going to include the citations in Flybase, then you need *F the following information: *F dTERA = Drosophila transitional Endoplasmic Reticulum ATPase, located at 46D. *F Dennis # *U FBrf0102090 *a Mozer *b B. *t 1998.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 17 15:01:11 1998 *F To: bmozer@codon.nih.gov *F Subject: highway *F Dear Brian, *F I am writing about the highway mutant that you obtained from Hugo Stocker *F and Ernst Hafen. Where you have published with it you have referred to it *F as an allel of Drop. *F Hugo and Ernst have an abstract about it for the upcoming Washington DC *F ADRC, where they refer to it as stghwy. Given the regulatory *F interactions, simple complementation tests might not have simple *F interpretations. *F Do you have any molecular information concerning where the lesion maps *F (with respect to stg, Dr or (preferably) both)? That might help. I have *F asked Ernst the same question. *F If there really is no way to settle it for the moment I will have to create *F an allele of stg called stghwy for the database and cross reference it to *F Drhwy until the situation resolves itself. *F I look forward to hearing from you, *F With best wishes, *F Rachel. *F From bmozer@codon.nih.gov Wed Mar 18 16:20:22 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: highway *F Dear Rachel, *F Regarding your questions concerning the hwy mutant and Drop, things *F are not completely clear at the moment. I agree that hwy is an allele of *F stg, but genetically it is also is an allele of Drop, but I don't know if *F hwy is also a double mutant, like the other Dr alleles. As I may have *F described in some of my meeting abstracts, Drop mutants are always double *F hits in two genes, one of which is stg and the other is distal to stg, and *F I refer to it as Dr-distal. *F Best regards, *F Brian Mozer # *U FBrf0102091 *a Mogila *b V. *t 1998.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:28:04 1998 *F To: mogila@rascal.med.harvard.edu *F Subject: ADRC: 236B *F Dear Vladic, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Maternal products of two new genes, rasp and grater, are required for the *F segmentation of the Drosophila embryo.' *F You describe two genes rsp and grr, that are new to FlyBase. Do you *F have map locations for these genes? It is nice if we can keep as many gene *F records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mogila@rascal.med.harvard.edu Thu Mar 19 00:01:31 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Re: 39th ADRC: 236B *F Dear Rachel, *F In the abstract 236B for the 1998 Drosophila Meeting I described two new *F genes, rasp (rsp), and grater (grr). *F grater (grr) is located between 068A02-03 and 068B01-03 *F This location is based on the following complementation tests: *F non-complementing deficiencies: *F Df(3L)lxd6 Breakpoints: 067E01-02;068C01-02 *F Df(3L)vin2 Breakpoints: 067F02-03;068D06 *F Df(3L)vin5 Breakpoints: 068A02-03;069A01-03 *F complementing deficiencies: *F Df(3L)vin4 Breakpoints: 068B01-03;068F03-06 *F Df(3L)vin7 Breakpoints: 068C08-11;069B04-05 *F So, in the 'FlyBase format' the location can be specified as follows: *F Left limit from inclusion within Df(3L)vin5 (FBab0002457) *F Right limit from non-inclusion within Df(3L)vin4 (FBab0002456) *F rasp (rsp) is located between 063A01 and 063C01 *F This location is based on the following complementation tests: *F non-complementing deficiencies: *F Df(3L)M21 Breakpoints: 062F;063D *F Df(3L)HR370 Breakpoints: 063A01;063D01 *F complementing deficiencies: *F Df(3L)HR232 Breakpoints: 063C01;063D03 *F Df(3L)HR119 Breakpoints: 063C06;063E *F Again, in the 'FlyBase format' the location can be specified as follows: *F Left limit from inclusion within Df(3L)HR370 (FBab0002327) *F Right limit from non-inclusion within Df(3L)HR232 (FBab0002324) *F This is all reliable information I can provide at this point. As soon as I *F verify and double-check position of these genes on the 'molecular map' I *F will send you an email with all data suitable for FB. *F With best wishes, *F Vladic. # *U FBrf0102092 *a Gubb *b D. *t 1998.3.20 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Mar 19 13:50:40 1998 *F To: d.gubb@gen.cam.ac.uk *F Subject: ADRC: 372A *F Dear David, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Serine protease/serpin balance regulates expression of the antifungal *F peptide gene drosomycin in Drosophila.' *F You describe three genes 'Ser1', 'Ser2' and 'Ser3', all new to FlyBase. *F Do you have a cytological location for these that has been determined *F independently of nec (such as by chromosome in situ hybridisation)? *F Is there a one to one correspondence between any one of the Ser genes and *F nec? *F Thank you for your help, *F with best wishes, *F Rachel. *F From dg27@mole.bio.cam.ac.uk Fri Mar 20 08:24:28 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 372A *F The ser cluster maps at 43A1.2 by in situ hybridisation. There is some *F evidence for Genetic complexity (Heitler et al 1993) but P element rescue *F indicates that Ser3 is necrotic. Deletion of Ser2 alone gives no detectable *F phenotype, while overlapping deletions of all 3 Serpins survives as an *F adult for a short while before dying with an extreme necrotic phenotype. *F David Gubb # *U FBrf0102093 *a Shim *b K. *t 1998.3.26 *T personal communication to FlyBase *u *F Archived # *U FBrf0102094 *a Taylor *b M.V. *t 1998.3.26 *T personal communication to FlyBase *u *F Archived # *U FBrf0102095 *a Abmayr *b S. *t 1998.3.26 *T personal communication to FlyBase *u *F Archived # *U FBrf0102096 *a Loo *b L. *t 1998.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 10 11:48:45 1998 *F To: lloo@fhcrc.org *F Subject: ADRC: 424A *F Dear Leonora, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Characterization of the Drosophila Homolog of Mad, A Transcriptional *F Repressor of the Myc-Max-Mad Network.' *F You describe dMad, a gene new to FlyBase. Do you have a cytological *F location for dMad? It is nice if we can keep as many gene records as *F possible anchored to the map. We do not prefix gene symbols with D for *F Drosophila. Unfortunately the symbol Mad is already taken, for 'Mothers *F against dpp', so perhaps you could suggest an alternative? We can help you *F find something if you could tell us the full name of the gene in question. *F Thank you for your help, *F with best wishes, *F Rachel. *F From lloo@fred.fhcrc.org Mon Mar 23 23:15:29 1998 *F To: Rachel Drysdale *F Subject: Re: ADRC: 424A *F Dear Rachel, *F My apologizes for the delay in this response. *F The dMad gene that we are presently characterizing has been cytologically *F mapped to the 3E region. *F I look forward to possibly meeting with you at the meeting this week. *F Sincerely, *F Lenora # *U FBrf0102097 *a Han *b Z. *t 1998.4.1 *T personal communication to FlyBase *u *F FAX 508-856-4289 *F Zhiqiang Han *F Re: ADRC: 014 *F Dear Zhiqiang, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'A conserved p38 MAP kinase pathway regulates Drosophila immunity gene expression' *F You describe Mkk3 and Mkk4, both new to FlyBase *F Abstract 344C (Takaesu et al, 'A novel p38 MAPK cascade, *F DmMEKK1-DmMKK3-DmMPK2, may function in Dpp/TGF-beta signaling pathway in *F Drosophila') also describe an 'MKK3'. Do you know whether this is the same *F gene as your Mkk3? *F Also, FlyBase has this gene entry already: *F \*a Mek3 *F \*x FBrf0085669 == Noselli and Glise, 1996, A. Conf. Dros. Res. 37: 354 *F \*x FBrf0086756 == Noselli, 1996.4.28, personal communication *F \*x FBrf0100395 == Suzanne, 1997, Int. J. Dev. Biol. 41(5): 28S *F \*z FBgn0015763 *F \*c 11D1--11D2 *F \*c Left limit from (method unavailable) (FBrf0086756) *F \*c Right limit from (method unavailable) (FBrf0086756) *F \*E FBrf0086756 == Noselli, 1996.4.28, personal communication *F \*c 11D1--11D2 *F \*E FBrf0085669 == Noselli and Glise, 1996, A. Conf. Dros. Res. 37: 354 *F \*e MEK-kinase 3 *F \*i DMEK3 *F \*j species == Mus; gene == Prkmk1; MGI:102562 *F \*j species == Homo sapiens; gene == PRKMK1; GDB:136418; OMIM:176872 *F \*F protein kinase == EC 2.7.1.37 *F \*d MAP kinase kinase (MAPKK) *F \*E FBrf0100395 == Suzanne, 1997, Int. J. Dev. Biol. 41(5): 28S *F \*i DMEK3 *F Does this correspond to your Mkk3? *F Do you have a cytological location for Mkk3 or Mkk4? It is nice if we can *F keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Zhiqiang.Han@ummed.edu Thu Apr 02 04:02:18 1998 *F To: rd120@gen.cam.ac.uk *F Subject: gene: MKK3 and MKK4 *F Dear Rachel: *F I went to fly meeting but didn't get chance to talk to other *F people to know if the MKK3 that I have is the actually same as other *F people's. The D-MKK3 I have is about 1 kb away *F from hep, 5' to 5'. It is in the region 11C4-D3 on X chromosome. D-MKK4 *F is in the region of 85A2-A7 on 3rd chromosome. I checked Drosophila gene *F bank but didn't find any sequences for MKK3 except for some sequence tags. I *F would assume that my *F D-MKK3 is the same as the one Noselli and Takaesu have based upon they *F are all mostly homologous to human MKK3 and MKK (65%). But I don't have *F sequence alignment to confirm it. *F Best regards *F Zhiqiang Han *F From rd120@gen.cam.ac.uk Thu Apr 02 13:05:09 1998 *F To: Zhiqiang.Han@ummed.edu *F Subject: Re: gene: MKK3 and MKK4 *F Dear Zhiqiang, *F In view of the nature of the encoded product and cytological location I *F will treat your D-MKK3 as the same gene as Mek3 in FlyBase. *F I will curate your mail as a personal communication to FlyBase to serve as *F a source for the lovely mapping data. I am presuming that you used *F chromosome in situ hybridization to determine the cytological locations. *F Please let me know if this is not the case. *F With best wishes, *F Rachel. # *U FBrf0102099 *a Roote *b J. *t 1998.4.7 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Apr 07 17:58:20 1998 *F Date: Tue, 7 Apr 1998 18:07:01 +0000 *F Hi Rachel, *F In(2L)TE35B-GV9 carries a wb allele, which I've called wbGV9 *F J # *U FBrf0102100 *a Szabad *b J. *t 1998.4.8 *T personal communication to FlyBase *u *F From szabad@comser.szote.u-szeged.hu Wed Apr 08 09:24:46 1998 *F To: rd120@gen.cam.ac.uk *F Subject: mus309 et al. *F Dear Rachel, *F In deficiency mapping I made use of the following Df(3R)s: M-Kx1, T-32, *F T-45, P10, kar.D3, P21, T-10, T-55, T-47, -79, T-61, T-7, E-229, kar.D1, *F kar.Sz-12, cu and M86D. Here are the details: *F I used two alleles of mus309, 2 and 3 and monitored female *F sterility. Both mutant alleles are sterile in combination with the *F following Df(3R)s: M-Kx1,T-32, T-45, P10, kar.D3, P21, T-10, T-47, *F E-79 T-7. The mus309 alleles are viable and fertile in combination *F with E-229, kar.D1, kar.Sz-12, kar.H10, cu and M86D. (T-55 and T-61 *F were not included.) *F The ck12 lethal alleles (e77, e177 and e294) are lethal in combination *F with M-Kx1, T-32, T-45, kar.D3, P21, T-10, E-79 and E-229. The ck12 *F alleles are viable, female and male fertile in combination with the *F following Df(3R)s: P10, T-61, kar.Sz-12, kar.H10, cu and M86D. *F It is interesting that Df(3R)T-47 is lethal in combination with e177, *F viable, female fertile but male sterile with e77 and e294. T-55 and *F T-7 were not included. *F Best wishes, Janos. # *U FBrf0102101 *a Marygold *b S. *t 1998.4.16 *T personal communication to FlyBase *u *F Date: Thu, 16 Apr 1998 11:21:04 -0700 *F From: Steven.Marygold@ns1.nimr.mrc.ac.uk (Steven) *F Subject: P277 stock info *F Dear Kathy Matthew, *F In response to some queries, Allan Spradling has emailed me to say that the *F P277 stock (l(3)neo40 gene) 'was discarded from their P element *F collection some time ago because their own analysis determined that the *F mutant phenotype was not caused by the P insertion'. *F Although you may want to check with Allan first, I think that this *F info should be added to the Flybase records. *F Steven Marygold. # *U FBrf0102102 *a Brown *b N. *c T. *d Volk *t 1998.4.21 *T personal communication to FlyBase *u *F From nb117@mole.bio.cam.ac.uk Mon Apr 20 16:17:57 1998 *F To: Rachel Drysdale (Genetics) *F and *F From lgvolk@wiccmail.weizmann.ac.il Tue Apr 21 12:28:14 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Subject: RE: groovin/kakapo *F FlyBase currently has gene entries for both groovin and kakapo. 'groovin' *F was initially defined as the antigen recognised by a monoclonal antibody *F (in Volk and Vijay Raghavan, 1994, Development 120(1): 59--70), not a *F mutant. This monoclonal still stains embryos deficient for the kakapo *F gene, and homozyous mutant kakapo embryos. However the groovin monoclonal *F antibody was used to isolate a cDNA which turns out to be a kakapo cDNA, *F and the antibody recognizes an immunoprecipitated Kakapo protein, therefore *F the antibody appears to recognise kakapo as well as other proteins. # *U FBrf0102103 *a Gelbart *b W.G. *t 1994.7.29 *T personal communication to FlyBase *u *F >From gelbart@morgan.harvard.edu Fri Jul 29 16:09:35 1994 *F Hi Aubrey, *F re dpps2: *F The L&Z Genes entry on dpp is correct (since I wrote it). *F What happened was: *F The allele that had been called shv1 was renamed dpps1 *F during the dpp Grand Edit. *F The allele that had been T(2;3)shvS1 was renamed T(2;3)dpps2 *F during same. *F The alleles that were In(2L)shvS2 and In(2L)shvS4 proved to *F be molecularly absolutely identical and we decided that *F there must have been some stock keeping accident. We only *F kept these as one mutant called In(2L)dpps4. *F All other shvSx alleles became dppsx, with the numbers maintained *F in order, except that shvS16 proved to be a dpphr allele *F and was renamed accordingly. That's why there is no *F dpps16. By the way, the capital S for shv alleles was *F for Danny Segal, the discoverer; the lower case s for *F dpps alleles is for shortvein. *F I trust this is more than you wanted to know. *F Bill # *U FBrf0102104 *a Kalderon *b D. *t 1998.3.12 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Mar 11 12:50:20 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 11 Mar 1998 12:50:20 +0000 *F To: ddk1@columbia.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Wed, 11 Mar 1998 12:58:46 +0000 *F Content-Length: 1575 *F Dear Dr. Kalderon, *F I am curating the following paper for FlyBase: *F Davis et al., 1998, Neuron 20(2): 305--315 *F (Postsynaptic PKA controls quantal size and reveals a retrograde signal *F that regulates presynaptic transmitter release in Drosophila). *F in which they use two constructs containing 'a mutant PKA regulatory *F subunit fused to the UAS promoter' which they obtained from you (they also *F reference Li et al., 1995, Cell 80: 553--562 for these constructs). *F The two constructs are: ( = superscript) *F 1) UAS-PKAinh1 (stated to formerly be UAS-PKABDK22) which carries a *F single mutation in a cAMP binding site. *F 2) UAS-PKAinh2 (stated to formerly be UAS-PKABGD) which carries mutations *F in two cAMP binding sites. *F a) Could you confirm that these are constructs using the Pka-R1 gene rather *F than the Pka-R2 gene. *F b) FlyBase has a record of a single UAS-PKA regulatory subunit construct *F from Li et al., 1995, Cell 80: 553--562. This is called UAS-R* in that *F paper. I would be grateful if you could tell me whether either *F UAS-PKAinh1 or UAS-PKAinh2 correspond to UAS-R*, or whether they are 3 *F separate constructs. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From ddk1@columbia.edu Wed Mar 11 18:52:20 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 11 Mar 1998 18:52:20 +0000 *F Date: Wed, 11 Mar 1998 14:00:44 -0500 (EST) *F From: Daniel D Kalderon *F Sender: ddk1@columbia.edu *F To: Gillian Millburn *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 2721 *F Dear Gillian, *F All of the constructs used RI. The UAS-R* of Li et al really *F refers to both constructs as both gave the same results in these exp'ts *F although BDK (as opposed to BGD) was used much more frequently (and for *F example is used exclusively in our latest paper, Ohlmeyer et al., 1997 *F in Genes & Dev). From reading your summary I realize that there must *F also be a mistake in the Davis paper since BDK has two mutations and BGD *F only one. This is probably not very important since both constructs work *F as inhibitors; mutation of both sites slightly reduces affinity for *F catalytic subunit in vitro and makes R entirely insensitive to cAMP- the *F single site mutant can be activated in vitro but by cAMP concentrations *F higher than those likely to be seen in vivo. Probably none of these *F details will ever be published so I realize that the UAS-R* will remain *F incompletely documented in the literature. *F Sincerely, *F Daniel Kalderon *F From gm119@gen.cam.ac.uk Thu Mar 12 15:00:13 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 12 Mar 1998 15:00:13 +0000 *F To: ddk1@columbia.edu *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Thu, 12 Mar 1998 15:08:38 +0000 *F Content-Length: 2386 *F Dear Daniel, *F thankyou for your prompt reply, it was very helpful. I have a few more *F questions to sort out the details of the two UAS-Pka-R1 constructs, and *F then if it is OK with you I would like to include the information about the *F two constructs in FlyBase as a personal communication from you. *F 1. *F I would like to confirm that: *F a) the constuct with a mutation in 1 cAMP site is called BGD *F b) the construct with mutations in 2 cAMP sites is called BDK *F 2. *F Would you prefer the constructs to be called: *F P{UAS-Pka-R1.BGD} and P{UAS-Pka-R1.BDK} *F or, as used in the Davis paper: *F P{UAS-Pka-R1.inh1} - for the construct with a mutation in 1 cAMP site *F P{UAS-Pka-R1.inh2} - for the construct with mutations in 2 cAMP sites *F I look forward to hearing from you, *F Gillian Millburn *F From ddk1@columbia.edu Thu Mar 12 21:56:45 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 12 Mar 1998 21:56:45 +0000 *F Date: Thu, 12 Mar 1998 17:05:10 -0500 (EST) *F From: Daniel D Kalderon *F Sender: ddk1@columbia.edu *F To: Gillian Millburn *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Content-Length: 4821 *F On Thu, 12 Mar 1998, Gillian Millburn wrote: *F > Dear Daniel, *F > *F > thankyou for your prompt reply, it was very helpful. I have a few more *F > questions to sort out the details of the two UAS-Pka-R1 constructs, and *F > then if it is OK with you I would like to include the information about the *F > two constructs in FlyBase as a personal communication from you. *F > *F > *F > 1. *F > I would like to confirm that: *F > *F > a) the constuct with a mutation in 1 cAMP site is called BGD *F > b) the construct with mutations in 2 cAMP sites is called BDK *F That is correct. The BGD mutation is in the second cAMP binding domain *F (more C-terminal) changing G321 to D. BDK additionally has a mutation *F G196 to E in the analogous position in the first cAMP binding domain> *F > 2. *F > Would you prefer the constructs to be called: *F > *F > P{UAS-Pka-R1.BGD} and P{UAS-Pka-R1.BDK} *F > Above is better because the Davis paper is a potential source of *F confusion and when I send out stocks they always say BDK or BGD *F > or, as used in the Davis paper: *F > *F > P{UAS-Pka-R1.inh1} - for the construct with a mutation in 1 cAMP site *F > P{UAS-Pka-R1.inh2} - for the construct with mutations in 2 cAMP sites *F > # *U FBrf0102266 *a Bloomington Drosophila Stock Center *b ?. *t 1997.1.30 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Shelagh Campbell *F To: Bloomington Drosophila Stock Center *F Subject: Df(2L)Dwee-delta5 *F Date: 30 January 1997 *F *F Information communicated: *F *F Aberration symbol Df(2L)Dwee-delta5 ('delta' in the symbol represents a greek delta) *F Breakpoints 27A;28A *F Comments very weak stock, deficiency is dominant female sterile, per S. Campbell *F # *U FBrf0102466 *a de Belle *b J.S. *t 1998.5.5 *T personal communication to FlyBase *u *F From nobody@morgan.harvard.edu Wed May 06 03:00:07 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 6 May 1998 03:00:07 +0100 *F Date: Tue, 5 May 1998 22:06:18 -0400 *F From: debelle@ccmail.nevada.edu (JS de Belle) *F Subject: FlyBase Help Mail *F To: flybase-help@morgan.harvard.edu *F Content-Length: 447 *F debelle@ccmail.nevada.edu (JS de Belle) sent the following *F comments to flybase-help: *F \------------------------------------------------------------ *F Concerning the cutlet gene on 2L, *F Flybase ID number FBgn0015376, *F the allele designations should be: *F clI1 *F clI7 *F clI10 *F clI14 *F clB13 *F JS de Belle *F \------------------------------------------------------------ # *U FBrf0102478 *a Nakagoshi *b H. *t 1998.5.11 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Tue Apr 29 09:15:00 1997 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Tue, 29 Apr 1997 09:15:00 +0100 *F To: nakagoshi@ncnaxp.ncnp.go.jp *F Subject: Help FlyBase - defective proventriculus *F Cc: eleanor@gen.cam.ac.uk *F From: Eleanor Whitfield (Genetics) *F Date: Tue, 29 Apr 1997 09:15:32 +0100 *F Content-Length: 474 *F Hi, *F At the Chicago meeting I approached you at your poster concerning the *F symbol dep for your gene defective proventriculus. Unfortunately the *F symbol is already being used for the gene depressed isolated by Bridges in *F 1913. *F Have you chosen an alternative symbol for the gene? *F Please check your new symbol in FlyBase: *F http://flybase.bio.indiana.edu/ *F or you find it easier to you the mirror in Japan: *F http://shigen.lab.nig.ac.jp:7081/ *F Regards, *F Eleanor Whitfield *F FlyBase *F From nakagosi@ncnaxp.ncnp.go.jp Mon May 11 17:03:25 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Mon, 11 May 1998 17:03:25 +0100 *F Date: Tue, 12 May 1998 01:17:46 +0900 *F To: Eleanor Whitfield (Genetics) *F From: nakagosi@ncnaxp.ncnp.go.jp (Hideki Nakagoshi) *F Subject: Re: Help FlyBase - defective proventriculus *F Mime-Version: 1.0 *F X-Mailer: Eudora-J(1.3.8.5-J13) *F Dear Ms. Eleanor Whitfield *F I am very sorry for delaying the response. *F We have just submitted our paper about the *F 'defective proventriculus' gene. *F We decided to use the symbol 'dve'. *F Sincerely yours, *F Hideki Nakagoshi # *U FBrf0102535 *a Gelbart *b W. *t 1998.4.30 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Apr 30 11:40:12 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 30 Apr 1998 11:40:12 +0100 *F To: rd120@gen.cam.ac.uk, gelbart@morgan.harvard.edu *F Subject: dpp4 *F From: Rachel Drysdale (Genetics) *F Date: Thu, 30 Apr 1998 11:49:10 +0100 *F Content-Length: 56 *F dpp4 = dpphr4. *F dpp4c is a typesetting artefact! *F Bill. # *U FBrf0102536 *a Abmayr *b S. *t 1998.5.1 *T personal communication to FlyBase *u *F >From rd120@gen.cam.ac.uk Tue Mar 10 13:00:25 1998 *F To: sma1@psu.edu *F Subject: ADRC: 668A *F Dear Barbara and Susan, *F I am writing in connection with your abstract for the upcoming Washington *F DC ADRC: *F 'Genetic characterization of sticks and stones, a gene involved in myoblast fusion.' *F You describe the new gene, sns, and give 2-58 as its map location. Could *F you tell us which flanking mutations you used in determining this map *F location, or (or as well as) the cytological location of sns if you have *F determined it by chromosome in situ or by mapping against aberrations. *F This is all so that sns can be placed on the genome map. It is nice if we *F can keep as many gene records anchored to the map as possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F >From sma1@psu.edu Fri May 01 22:10:26 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC: 668A *F Rachel, *F 1. sns is uncovered by Df(2R)Np3, which is a stock in the collection. *F 2. sns is not uncovered by 3646 - In(2LR)P14LTE45FR. This is also a *F stock in the collection that has been mapped cytologically. It overlaps *F Df(2R)Np3 genetically, and establishes the proximal breakpoint of the *F region that uncovers sns. *F 3. sns is not uncovered by Df(2R)Np1. This is a third stock in the *F collection that has been mapped cytologically. It also overlaps Df(2R)Np3 *F genetically, and establishes the distal breakpoint of the region that *F uncovers sns. *F Additionally, before we generated or obtained deficiencies that uncovered *F sns, we mapped it by recombination frequency to genetic position 58 using *F both Bl and cn on the proximal side and L and sca on the distal side. *F Finally, you asked about our reference to the ryanodine receptor. Yes this *F the same thing as Rya-r44F (I think we used the terminology in the *F published papers). It is also missing in Df(2R)Np3 but present in 3646 and *F Df(2R)Np1. *F Susan *F Susan M. Abmayr, Ph.D. *F Asst. Prof. of Molecular Genetics *F Dept. of Biochemistry and Molecular Biology *F The Pennsylvania State University *F 459 North Frear Lab *F University Park PA.16802 *F Phone: 814-863-8254 *F FAX: 814-863-7024 *F email: sma1@psu.edu # *U FBrf0102537 *a Zeng *b C. *t 1998.4.21 *T personal communication to FlyBase *u *F From zeng@itsa.ucsf.edu Wed Apr 22 00:40:43 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F To: flybase-help@morgan.harvard.edu *F Subject: Gal4 line 109-68 *F We wish to add to the flybase some details about an enhancer trap Gal4 *F line 109-69 (FlyBase ID number: FBal0052395, transposon flybase ID *F number: FBti0004036). This line was isolated in Jan's lab, and was used in several *F publications. The most precise description of this line is by Dan *F Doherty's 1997 paper (Doherty, D., Jan, L.Y., Jan, Y.N. Development v124, *F 3881--3893. Flybase ID FBrf0099033), i.e. 'it is expressed in a subset of *F proneural cluster cells, SOP's and their daughters '. In our hands, 109-68 *F is expressed in some proneural cluster such as the ones in the scutellar *F region and predominantly (if not exclusively) in the SOP and their *F daughters for most of the bristles elsewhere in the notum. In making *F mosaics with UAS-flp, and 109-68, we can get clones that are only in the *F sense organ lineage (the surrounding epidermal cells were marked so we *F know). There are also cases in which the clones include nearby epidermal *F cells. In other publications, we have stated that 109-68 is SOP specific, *F which appears to be a over-simplification. We hope that by providing this *F information through the flybase, the potential confusion on this line can *F be minimized. *F Thank you. *F Chaoyang Zeng Ph. D. *F Jan's Lab *F University of California at San Francisco # *U FBrf0102538 *a Roote *b J. *t 1998.5.11 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon May 11 10:57:39 1998 *F To: rd120@mole.bio.cam.ac.uk *F Hello Rachel, *F Michael (Ashburner) refined this cytology in about 1985 - sorry I forgot to *F tell you sooner. *F Aberration symbol In(2L)b81f3 *F Breakpoints 34D5-34D8;35B10 *F It's now 34D3;35D5-7. *F John # *U FBrf0102539 *a Giniger *b E. *t 1998.5.13 *T personal communication to FlyBase *u *F From eginiger@fred.fhcrc.org Tue May 12 18:18:05 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase *F Dear Rachel, *F The UAS-Notch(deltaE) (also called ECN) in Doherty, and in Baker and *F Schubiger, is actually the one from Larkin, which I know because I made it *F and gave it to all of them. *F I hope that helps. *F Ed *F From rd120@gen.cam.ac.uk Wed May 13 08:55:30 1998 *F To: eginiger@fred.fhcrc.org *F Subject: Re: Helping FlyBase *F Hi Ed, *F A ha, I think we have captured two out of three of these as fusion genes *F with Dl, and the other as an allele of N. Are these the ones you mean? *F \*A Dl::NUAS.cDa *F \*x FBrf0086385 == Doherty et al., 1996, Genes Dev. 10(4): 421--434 *F \*A Dl::N&Dgr;ECN.UAS *F \*x FBrf0086321 == Baker and Schubiger, 1996, Development 122(2): 617--626 *F \*A N&Dgr;EN.UAS *F \*x FBrf0090659 == Larkin et al., 1996, Development 122(11): 3639--3650 *F If these are all the same I will merge the allele records so that they *F don't look like three separate alleles. *F Rachel. *F From eginiger@fred.fhcrc.org Wed May 13 18:05:10 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase *F Rachel, *F The three ECN references you picked are indeed the correct ones, and *F yes, they represent a gene fusion to the Dl signal sequence (which means *F that the final protein is actually NOT a fusion, since the connection is *F right at the signal cleavage site. But I digress). You'll also be seeing *F this construct appear one more time when Fuerstenberg and Giniger actually *F comes out in DB in a couple of months. That will finally describe the *F construction. Oh, and by the way, contrary to what some of the published *F descriptions say, the construct also does NOT express the complete Notch *F intracellular domain -- it picked-up a frameshift at nt 7448 (numbering of *F Wharton and Artavanis), causing the protein to truncate at lysine 2235, *F append the sequence RPPT, and then stop. As far as we can tell, this *F doesn't remove any biological function, and actually seems to make the *F protein a bit more active than a full-length ECN. All of this is described *F in the upcoming Fuerstenberg paper. *F Ed # *U FBrf0102540 *a Brookman *b J. *t 1998.5.13 *T personal communication to FlyBase *u *F From jjb@mole.bio.cam.ac.uk Wed May 13 14:13:36 1998 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase *F On Wed, 13 May 1998, Rachel Drysdale wrote: *F > Hi Jenny, *F > *F > seems like an age ago but we talked about this in DC. Here is what we have *F > for Ring. You were going to send me a refined cytological location, *F > determined by chromosome in situ, since this range is so large. *F > *F > *F > \*a Ring *F > \*i Ding *F > \*i Dring *F > \*i Rnf2 *F > \*g AJ001514; e340043 *F > \*c 98A1--99D9 *F > \*c Left limit from sequence databank entry AA001514 *F > \*c Right limit from sequence databank entry AA001514 *F > \*j species == Homo sapiens; gene == RNF2; GDB:6240419 *F > \*J Zinc finger, C3HC4 type, protein. *F > \*z FBgn0020911 *F > *F > Rachel. *F Dear Rachel, *F About the cytological location for Ring: *F The locations are approximately 98B and 99C as determined by chromosome *F in situs with a dEST sequence LD 6636. *F The EST probe definitely gave two bands. The 98B was very strong and the *F 99C just a little weaker. We quizzed the person who sequenced the probe *F and concluded that it may represent a repetitive sequence rather than a *F hybrid clone. *F Jenny # *U FBrf0102541 *a Munroe *b S. *c S. *d Paine-Saunders *e S. *f Stowers *g A. *h Rascle *t 1998.5.12 *T personal communication to FlyBase *u *F Archived. # *U FBrf0102542 *a Roote *b J. *t 1998.5.15 *T personal communication to FlyBase *u *F From: John Roote *F Date: Fri, 15 May 1998 10:09:08 +0100 *F Subject: Re: Alexandrov *F Df(2L)b89e12 is b- -- l(2)34De-, l(2)34Dg+ *F i.e. it orders the previously unordered De and Dg. *F John *F From rd120@gen.cam.ac.uk Fri May 15 10:24:34 1998 *F To: jr32@mole.bio.cam.ac.uk *F Subject: Re: Alexandrov *F we don't have a 'Df(2L)b89e12' *F we only have a *F \*a T(2;3)b89e12 *F Is this what you mean? *F R *F From jr32@mole.bio.cam.ac.uk Fri May 15 10:43:30 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Alexandrov *F 'Fraid so *F T(2;3)b89e12 is deleted for b, 34Dc, 34Dd, 34Df, 34De but not 34Dg. *F J *F From rd120@gen.cam.ac.uk Fri May 15 11:19:30 1998 *F To: jr32@mole.bio.cam.ac.uk *F Subject: Re: Alexandrov *F >T(2;3)b89e12 is deleted for b, 34Dc, 34Dd, 34Df, 34De but not 34Dg. *F So its a three break event in fact, a Deficient Translocation. *F Is the Df visible or does it still look like a simple two break event? *F The breaks we have are basically \*B 34D4;79C3, but if you could add a third *F break to that you'd make a curator happy. *F Rach *F From jr32@mole.bio.cam.ac.uk Fri May 15 11:27:46 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Alexandrov *F We haven't done the cytology - Alex just says 34D4;79C3 and I suspect Df *F 34D4; approx 34D6 would be easily missed within a T(2;3). *F J # *U FBrf0102637 *a Bloomington Drosophila Stock Center *b ?. *t 1998.6.2 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Ruf[1] *F Date: 2 June 1998 *F *F Information communicated: *F *F Gene symbol Ruf *F Allele symbol Ruf[1] *F Map location chromosome 2 *F Phenotype visible | dominant; slightly rough eye, leathery and warped wings, with some wing vein deltas *F Discoverer E.B. Lewis *F Comment This allele was in the Mid-America collection and has been transferred to Bloomington. # *U FBrf0102810 *a Das *b P. *c S.H. *d Cho *t 1998.6.17 *T personal communication to FlyBase *u *F From das_m_pradeep@raven.rutgers.edu Wed Jun 17 22:17:50 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 Jun 1998 22:17:50 +0100 *F Date: 17 Jun 1998 17:27:12 -0500 *F From: 'Das, Pradeep' *F Subject: Re. FlyBase query *F To: 'Gillian Millburn' *F Cc: 'rick' *F X-Mailer: Mail*Link SMTP-MS 3.0.2 *F Content-Length: 1031 *F Dear Gillian, *F The tkv* construct used for the experiments described in our paper (see below) *F is indeed downstream of UAS elements. However, the expression of the construct *F was not driven by a GAL4 line, but rather by enhancer piracy, and hence the *F promoter is unknown. The only information we are in a position to provide is *F that the line used is a jump of UAS-tkv* onto the TM3, Sb balancer chromosome. *F Please feel free to write to me for further clarifications. *F Regards, *F Pradeep Das *F >Dear Dr. Padgett, *F > *F >I am curating your paper for FlyBase: *F > *F >Das et al., 1998, Development 125(8): 1519-1528 *F > *F >and I have a question about the activated tkv* construct used in your *F >paper. *F > *F >Is this construct expressed using UAS regulatory sequences, and if *F >so, was expression driven using enGAL4 - as for the activated sax* *F >construct. If the tkv* construct is not expressed under the control *F >of UAS regulatory sequences, what promoter sequences drive its *F >expression ? *F > *F >I look forward to hearing from you, *F > *F >Gillian Millburn *F From gm119@gen.cam.ac.uk Thu Jun 18 10:46:12 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jun 1998 10:46:12 +0100 *F To: das'_m_pradeep@raven.rutgers.edu *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 18 Jun 1998 10:56:19 +0100 *F Content-Length: 503 *F Dear Pradeep, *F thanks for your reply, it was very helpful. *F I would just like to confirm whether the UAS-tkv* construct used to make *F your tkv* enhancer piracy line is the construct that was described in: *F Lecuit et al., 1996, Nature 381(6581): 387--393 *F I would like to record the details of your tkv* enhancer piracy line as a *F personal communication to FlyBase from you. Is that OK ? *F Also, do you have a particular designation for the line, as this helps in *F naming the construct in FlyBase. *F Gillian *F From das'_m_pradeep@raven.rutgers.edu Thu Jun 18 11:15:01 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jun 1998 11:15:01 +0100 *F Date: 18 Jun 1998 06:24:51 -0500 *F From: 'Das, Pradeep' *F Subject: RE: FlyBase query *F To: 'Genetics' *F Cc: 'rick' , *F 'Cho, Seo-Hee' *F X-Mailer: Mail*Link SMTP-MS 3.0.2 *F Content-Length: 1221 *F Dear Gillian, *F the UAS-tkv* construct described in our paper was made in our lab, so while the *F nature of the mutation may be the same as the one in Lecuit et al. (and in *F several other papers), the transgenic line itself is unique. The designation we *F use for this line is 'SC143' (TM3, Sb, tkv*). *F Please cite personal communication from Seo-Hee Cho - he's the student in our *F lab that made the lines and characterised them. *F Hope that was helpful! Please feel free to email me for further clarifications. *F Regards, *F Pradeep *F From gm119@gen.cam.ac.uk Thu Jun 18 13:09:48 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jun 1998 13:09:48 +0100 *F To: das'_m_pradeep@raven.rutgers.edu *F Subject: RE: FlyBase query *F Cc: padgett@waksman.rutgers.edu, cho@waksman.Rutgers.EDU, gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 18 Jun 1998 13:19:48 +0100 *F Content-Length: 512 *F Dear Pradeep, *F as you made the UAS-tkv* construct in your lab, I will make a new entry in *F FlyBase for it. Is the mutation Q199D, as described in the materials and *F methods of your paper for Ubi-tkv ? and is it in the pUAST vector ? *F As I've been writing to you, it seems fair if I cite the personal *F communication from both Seo-Hee Cho and you, and I'll record that the *F construct was made by him in the personal communication, is that OK ? *F Sorry to keep e-mailing you, and thanks for being so helpful ! *F Gillian *F From das'_m_pradeep@raven.rutgers.edu Thu Jun 18 15:04:33 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jun 1998 15:04:33 +0100 *F Date: 18 Jun 1998 10:13:44 -0500 *F From: 'Das, Pradeep' *F Subject: RE: FlyBase query *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-MS 3.0.2 *F Content-Length: 675 *F dear gillian, *F >FlyBase for it. Is the mutation Q199D, as described in the materials and *F >methods of your paper for Ubi-tkv ? and is it in the pUAST vector ? *F yes to both. *F regards, *F pradeep # *U FBrf0102814 *a Bullard *b B. *t 1998.5.11 *T personal communication to FlyBase *u *F Archived # *U FBrf0102816 *a Bloomington Drosophila Stock Center *b ?. *t 1998.5.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon May 11 17:47:41 1998 *F To: flybase-updates@morgan.harvard.edu *F Subject: mus113 and mus114 *F Hi folks-- *F I need you to make two new gene entries. We received a mus113RT3 and a *F mus114RT1 stock from Bowling Green that we will soon put in the *F Bloomington collection. These mutagen-sensitive mutations were generated *F by Jim Boyd, but I don't think they appear in any of his publications (I *F have to admit I haven't looked). They are X-linked. I have no other *F information about them. *F Thanks, *F Kevin # *U FBrf0102817 *a Sekelsky *b J. *t 1998.5.21 *T personal communication to FlyBase *u *F From jjsekelsky@ucdavis.edu Thu May 21 17:12:48 1998 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase *F Hi Rachel. I'm probably not going to be much help on these stocks. All I *F know is that they're from the Yamamoto et al., Mut Res. (1990) screen, *F although they're not published in that paper. *F Jeff *F > There are two stocks that its possible you could help us out with. *F > They are: *F > *F > BG stock number: 1153.6 *F > Stock: mus113RT3/C(1)DX, y* f1 *F > Chromosome 1 *F > Received from: J.B. Boyd *F > Date received: 5/28/91 *F > Comments: May not be right *F > *F > BG stock number: 1153.7 *F > Stock: mus114RT1/C(1)DX, y1 f1 *F > Chromosome 1 *F > Received from: J.B. Boyd *F > Date received: 5/28/91 *F > *F > The reason I am writing to you, particularly, is your *F > \*x FBrf0084352 == Sekelsky et al., 1995, Genetics 141(2): 619--627 *F > paper where you discuss 'mei-9RT1', and say that the work began in Boyd's *F > lab. I know that there are several such RT1 alleles, but in any case *F > you may be able to add something to the data we have for mus113 and *F > mus114. *F > *F > The kinds of information I am looking for are: *F > Do mus113 and mus114 correspond to genes known by any other means? *F > Has anything been published about them that we might have missed? *F > What is known about their map location? *F > What mutagen generated the alleles? *F > What is known about their mutant phenotype? *F > *F > with best wishes, *F > Rachel. # *U FBrf0102818 *a Bloomington Drosophila Stock Center *b ?. *t 1998.5.21 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: FM7k *F Date: 21 May 1998 *F *F Information communicated: *F *F Aberration symbol In(1)FM7 *F Markers on variant y[31d] sc[8] sn[X2] B[1] *F Variant symbol FM7k *F Reference Loring Craymer, personal communication to Bloomington Stock Center *F Comments female sterile, balancer # *U FBrf0102820 *a Bloomington Drosophila Stock Center *b ?. *t 1998.5.16 *T personal communication to FlyBase *u *F From matthewkfly.bio.indiana.edu Sat May 16 03:47:20 1998 *F To: flybasemorgan.harvard.edu *F Subject: balancer stuff *F Here is the list of balancer genotypes that need to be added or *F corrected. *F FBba0000014 (Basc) *F full genotype: sc8 scS1 wa B1 *F FBba0000015 (Bascy) *F full genotype: y82 sc8 scS1 wa B1 *F FBba0000064 (LVM) *F add genotype: l(3)LVML1 pe1 l(3)LVMR1 *F FBba0000010 (FM0) *F full genotype: y31d sc8 w1 vOf m2 f1 B1 *F FM0 is indeed w1. It was made beginning with a In(1)dl-49 marked with *F w1. *F It is only vOf. The v1 _is_ a misprint in L&Z (as is mei9L1 on M9). *F FBba0000011 (FM1) *F correct genotype: y31d sc8 wa lzs B1 *F (unless you know the L&Z record to be wrong about the presence of B1) *F FBba0000012 (M6) *F full genotype: y31d sc8 wa vOf f1 *F FBba0000013 (M9) *F full genotype: y31d sc8 wa mei-9L1 vb1 vOf f1 *F FBab0010487 (Biny) *F correct genotype: y31d sc- vOf f1 B1 *F (not sc1) *F FBba0000014 (Basc) *F full genotype: sc8 scS1 wa B1 *F FBba0000015 (Bascy) *F full genotype: y82 sc8 scS1 wa B1 *F FBba0000038 (SM5) *F correct genotype: al2 ds55 Cy1 ltv cn2 sp2 *F (ds allele per FlyBase) *F FBba0000039 (SM6a) *F correct genotype: al2 Cy1 dplvI cn2P sp2 *F FBba0000068 (MRS) *F full genotype: M(3)76A1 ry2 Sb1 *F FBba0000042 (TM1) *F correct genotype: Me1 ri1 Sbsbd-l *F FBba0000047 (TM3) *F correct genotype: ri1 pp sep1 l(3)89Aa1 Ubxbx-34e e1 *F (L&Z show y+, but many TM3's are no longer y+) *F FBba0000057 (TM6B) *F correct genotype: AntpHu e1 *F FBba0000072 (TMS) *F correct genotype: M(3)76A1 kar1 ry2 Sb1 P{Delta2-3} # *U FBrf0102823 *a Bloomington Drosophila Stock Center *b ?. *t 1998.6.2 *T personal communication to FlyBase *u *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Info for curation *F Hi folks-- *F Here's some info that came along with new stocks. *F 1. Df(2R)Chig230 *F Patrick Morcillo (p-morcillo@ski.mskcc.org) in Dale Dorsett's lab says this *F Df removes orange, but not speck. Also lethal over l(2)00628 and *F l(2)05006, but not l(2)01296. *F 2. Nancy Roche in Thom Kaufman's lab screened for EMS-induced lethals over *F Df(3R)96B and found 8 complementation groups. Df(3R)96B was isolated in *F Barry Ganetsky's lab and removes 96A21 to 96C2. The Df complements tolkein, *F but not tolloid (Nancy cited Nguyen et al., 1994 for this bit of compl. *F data). Nancy's 8 compl. groups are: *F tld 6 alleles; tld18 thru 23 *F l(3)96Ba 4 alleles; 1 thru 4 *F l(3)96Bb 2 alleles; 1 and 2 *F l(3)96Bc 1 allele; 1 *F l(3)96Bd 1 allele; 1 *F l(3)96Be 1 allele; 1 *F l(3)96Bf 1 allele; 1 *F l(3)96Bg 1 allele; 1 *F This work in described in her Ph.D. dissertation, Indiana U., 1998, Chapter *F 5. *F Muchos gracias!! *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 812-855-5782 *F Indiana University *F Bloomington, IN 47405-6801 # *U FBrf0102824 *a Paro *b R. *t 1998.6.8 *T personal communication to FlyBase *u *F To: Rachel Drysdale (Genetics) *F From: Renato PARO *F Subject: Re: Help FlyBase - sd mutation *F >I am curating a paper of yours for FlyBase: *F >Cavalli and Paro, 1998, Cell 94(4): 505 *F >in which you discuss an insertion of P{5F24} into upstream sequences of *F >gene sd, causing a mutation of sd (Figure 5, pg 510). *F > *F >Unfortunately you have not assigned a mutant designation for this allele. *F >Without a mutant designation I cannot capture the phenotypic data you have *F >provided. *F > *F >Could you please send me the designation you would like used. *F Dear Rachel Drysdale, *F I would like to give the following designation to the alleles created by *F the P-insertion in the scalloped gene: *F line fLW-1: sd CMM1 *F line FLW-1: sd CMM2 *F Both lines have an integration of the Fab-7 carrying transgene in the *F promoter region of sd and depict a sd phenotype. *F With best wishes, *F Renato PARO *F ZMBH *F University of Heidelberg *F Im Neuenheimer Feld 282 *F 69120 Heidelberg *F Germany *F Tel: +49-6221-54 68 78 *F Fax: +49-6221-54 58 93 # *U FBrf0102825 *a Reichhart *b J.M. *c D. *d Ferrandon *t 1998.6.8 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: J.-M.Reichhart and D. Ferrandon, UPR CNRS, Strasbourg *F To: Bloomington Drosophila Stock Center *F Subject: Green Balancers *F Date: 4 June 1998 *F *F Information communicated: *F We have used the S65T green fluorescent protein (GFP) as a vital reporter to introduce a dominant innocuous marker onto the balancers of the *F three major chromosomes of D. melanogaster. *F *F Construction : The drosomycin promoter contained in pJM802 was replaced by the distal actin 5C promoter as an EcoRI-NheI fragment originating from pPac *F in which an NheI linker was inserted into the polylinker. The P element mediated transformation plasmid derived from pCaSpeR contained the actin 5C *F promoter, followed by the S65T version of the GFP and the drosomycin terminator. The nucleotide sequence of the transformation vector is available *F upon request. Transgenic fly lines were established as described. One of the P element insertion obtained was remobilized using a Delta(2-3) source of *F transposase. Insertions in FM7 (FM7i : , CyO and TM3 balancer chromosomes were selected. *F *F The following stocks were sent to the Bloomington stock center : *F - FM7i-pAct-GFP : *F C(1)DX,f/FM7,y[93j],sc[8],w,oc,ptg,B,P[w+mC act::GFP =pActGFP] *F - CyO-pAct-GFP: *F w; In(2LR)noc[4L],Sco[rv9R],b / In(2LR)O,Cy,dp[lvI],pr,cn[1],P[w+mC act::GFP =pActGFP] *F - TM3-pAct-GFP : *F w; Sb[1] / In(3LR)TM3, ri,pp,sep,l(3)89Aa,bx34eSer,P[w+mC act::GFP =pActGFP] *F *F Expression pattern : *F Since their cuticle is transparent, third instar larvae carrying the marked balancers are easy to score under the fluorescent dissecting *F microscope. The main GFP expression pattern consists of a strong fluorescence in the salivary duct, the copper cells, the proventriculus and the visceral *F musculature of the midgut. A weaker signal can be detected in imaginal disks. In first instar larvae, the fluorescence appears to be restricted to the *F midgut . Adult flies carrying GFP balancers can be recognized by a deep pseudopupil type of expression in the eye, a mild fluorescence in the *F proboscis and a strong signal in the abdomen. Upon dissection, it appears that the abdominal fluorescence is due to : *F - GFP expression in the reproductive tract of the male ; *F - GFP expression in ovaries (yolk of mature stages and musculature of the ovary sheath) and in the seminal receptacle in females. In many animals, the visceral musculature of the midgut is also fluorescent. *F In the embryo, there is a strong maternal contribution which masks the zygotic expression until about stage 15 of development, when a weak signal *F can be detected in the midgut, as in first instar larvae. In the absence of this maternal contribution, the expression of GFP can first be detected *F around 12h after egg laying. Selected pictures showing these expression patterns can be viewed at http://ibmc.u-strasbg.fr/upr9022/GreenBalancers.html *F *F In conclusion, these green balancers constitute a highly useful tool to score living larvae, pupae, and adult flies, especially when working with mutations *F on the second chromosome. # *U FBrf0102826 *a Bloomington Drosophila Stock Center *b ?. *t 1998.6.3 *T personal communication to FlyBase *u *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: bonpotpmeltaaytrn *F Hi-- *F Can you add some synonyms for some of our new stocks? *F l(3)S048706 as a synonym for bonS048706 *F l(3)S114307 as a synonym for potpS114307 *F l(3)S144114 as a synonym for meltS144114 *F l(3)S042314 as a synonym for aayS042314 *F l(3)S064117 as a synonym for trnS064117 *F Reference: FBrf0099763 *F Thanks *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 812-855-5782 *F Indiana University *F Bloomington, IN 47405-6801 # *U FBrf0102827 *a Roote *b J. *t 1998.6.3 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Wed Jun 03 19:47:39 1998 *F To: Terence Davis , *F Michael Ashburner *F Subject: GT6 *F Cc: rd120@mole.bio.cam.ac.uk *F Looking back at comments in old data sheets I'm fairly certain that GT6 *F breaks within wb. It has a weak wb-like phenotype over wb- deletions - but *F not visible over wb alleles (actually only one). *F The synthetic deletion (GT6 GR18) has very blistered wings over wb- deletions. *F i.e. GT6 is a 'new' wb allele *F J # *U FBrf0102839 *a Ellis *b H. *t 1998.6.17 *T personal communication to FlyBase *u *F From: hellis@mail.ncifcrf.gov (Hilary Ellis) *F Subject: FlyBase Help Mail *F To: flybase-help@morgan.harvard.edu *F hellis@mail.ncifcrf.gov (Hilary Ellis) sent the following *F comments to flybase-help: *F \------------------------------------------------------------ *F We have recently shown that polychaetoid and tamou are the same gene. *F Two of our pyd mutations, pydC5 and pydJ2 map molecularly to the *F transcription unit described as tam. In addition Xierong Wei in my lab has *F shown that the heat shock driven tam cDNA (provided by Ryu Ueda) rescues *F the phenotype of pydC5. We have also shown that pydC5/pydC5 embryos lack *F reactivity with an anti-tam antibody. This data is currently unpublished, *F but will be published in the near future. *F Hilary Ellis *F \------------------------------------------------------------ *F Server protocol: HTTP/1.0 *F Remote host: 539saw2.ncifcrf.gov *F Remote IP address: 129.43.18.48 # *U FBrf0102853 *a Greenspan *b R. *t 1998.6.18 *T personal communication to FlyBase *u *F Archived # *U FBrf0102854 *a Matthews *b K. *t 1998.7.24 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: P{Cdk7[P140S]}SL1 and P{snf[+],dhd[+]}SL2 *F Date: 24 July 1998 *F *F Background: FlyBase requested information from us on map location, viability and fertility of these insertions *F *F Information communicated: *F *F These two insertions are described in Larochelle et al., Genes & Development 12(3):370-381. *F *F Symbol used by authors Pw+[Dmcdk7[P140S]] *F FlyBase symbol P{w[+mC]=Cdk7[P140S]}SL1 *F Map location chromosome 3 *F Phenotype Rescues Cdk7[-] at 18o but not at 27o. It is presumably homozygous viable on its own. *F *F Symbol used by authors Pw+[snf+,dhd+] *F FlyBase symbol P{w[+mC]=snf[+],dhd[+]}SL2 *F Map location chromosome 1 *F Phenotype Probably viable on its own, but I'm not certain of that. We have it on a chromosome with Df(1)JB254 (deletes Cdk7, snf, and dhd), which is recessive lethal. *F *F # *U FBrf0102855 *a Chiba *b A. *t 1998.5.20 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Akira Chiba *F To: Bloomington Drosophila Stock Center *F Subject: P{UAS-gapGFP} *F Date: 20 May 1998 *F *F Information communicated: *F *F "My lab has generated P(UAS-gapGFP) transformant flies. gapGFP is a modified GFP, a fusion of the myristylatioin sequence from GAP43 gene to GFP, that is designed to target the GFP to cell memebrane. At the recent CSH drosophila neurobiology meeting, we reported their use in visualizing the fine memebrane structures of various cell types such as neurons and muscles." *F *F Additional information provided by A. Chiba: *F The construct is a modification of P{GawB}, starting with pwG17 from the C. Goodman lab. The myristylation sequence is from the human GAP43 (as described and constructed by Moriyoshi et al. NEURON (1994) 16:255-260). The GFP itself has the S65A mutation (per Morisyoshi et al.) and is visible with a regular FITC filter set. *F # *U FBrf0102856 *a Laverty *b T. *t 1998.7.28 *T personal communication to FlyBase *u *F *F Personal communication from Todd Laverty to Bloomington Stock Center *F *F A Bloomington Stock Center user reported that Df(2L)s1402/CyO flies (stock *F 556) crossed to w flies produced red-eyed progeny and asked if a w[+] *F transgene was in the stock. The Df had been analyzed by Todd Laverty, so *F he was contacted for information. *F *F Todd Laverty's response on July 27, 1998 to my query was: *F "Df(2L)s1402 was/is part of the Scott lethal P collection and has a P *F insert at 30B9-10 [originally we called it l(2)s1402]. In mapping the P, I *F noticed an associated Df in the stock [thus the name change?]. Since the *F Scott collection is marked with P{w[+]} what [the user] wrote makes perfect *F sense." *F *F Consequently, an insertion entry should be created for P{w[+mC]=lacW}s1402 *F and the Bloomington stock 556 should be denoted Df(2L)s1402, *F P{w[+mC]=lacW}s1402/CyO if the P{w[+mC]=lacW}s1402 notation is consistent *F with FlyBase standards. *F *F Kevin Cook *F Bloomington Drosophila Stock Center *F July 29, 1998 *F *F # *U FBrf0103137 *a Bellen *b H. *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Hugo Bellen *F To: Bloomington Drosophila Stock Center *F Background: patterns of insertions of lacZ reporter constructs *F Information communicated: *F *F "Insertion","Embryo","Larva","Adult Ovary" *F "P{lArB}A215.1M3",,,"germarium (region 3), nurse cells (stage 10)" *F "P{lArB}A229.1F1",,"brain, all discs, uniform", *F "P{lArB}l(3)B48.1M3[1]","amnioserosa & dorsal epidermis boundary, double row of cells in dorsal epidermis",,"follicle cells (stage 9), epithelial sheath, muscular sheath, trachea" *F "P{lArB}puc[A251.1F3]","amnioserosa & dorsal epidermis boundry, double row of cells in dorsal epidermis",,"follicle cells (stage 10)" *F "CyO, P{lArB}A480.1M2","amnioserosa, heart",,"follicle cells (stages 12 & 13)" *F "P{lArB}A355.1F3","amnioserosa, heart",,"germarium (region 2), nurse cells (stage 8), oocyte nucleus (stage 10B), ooplasm (stage 13)" *F "P{lArB}A308.1M3","amnioserosa, heart, head, hindgut, CNS subset",,"no staining" *F "CyO, P{lArB}A350.1M2","CNS, anal pads (rim), salivary glands, head, amnioserosa & dorsal epidermis boundary",,"follicle cells, border cells" *F "CyO, P{lArB}A79.1M2","fat body","brain, leg disc, wing disc", *F "P{lArB}A49.1M2","fat body (stage 16), blood cells (stage 17)",, *F "CyO, P{lArB}A109.1F2","fat body, blood cells",,"follicle cells" *F "P{lArB}A374.2F1","hindgut, anal pads, head, epidermis",,"no staining" *F "P{lArB}A154.2M3","malpighian tubules, head",,"no staining" *F "P{lArB}A289.1F1","malpighian tubules, head, CNS subset, PNS",,"germarium, follicle cells, border cells" *F "CyO, P{lArB}A203.3F2","malpighian tubules, intersegmental pits",,"germarium (regions 1 & 2?)" *F "P{lArB}A409.1F3","mesoderm, trachea",, *F "P{lArB}A168.1F1","midgut subset, CNS subset, PNS, head",,"epithelial sheath" *F "CyO, P{lArB}A32.1M2","midgut subset, head, posterior spiracles",,"nurse cells (stages 9-11), ooplasm?" *F "P{lArB}A292.2F3","midgut subset, proventriculus, gastric caecae",,"germarium (regions 1-3), follicle cells (stages 1-14), squamous follicle cells (stages 9-14)" *F "P{lArB}B72.1M3","midgut subset, proventriculus, hindgut, posterior spiracles, anal pads",,"follicle cells (stages 9-14), squamous follicle cells (stages 12-14)" *F "P{lArB}A490.2M3","midgut, head",, *F "CyO, P{lArB}A336.1F2","midgut, head",,"germarium (region 2), border cells (stages 9 & 10)" *F "P{lArB}A119.1M3","midgut, head, epidermis",,"follicle cells (stage 8)" *F "P{lArB}A487.1M3","muscle",,"no staining" *F "P{lArB}B21.1M3","muscle",,"no staining" *F "P{lArB}A418.1F1","muscle, PNS, head",,"no staining" *F "P{lArB}l(3)A519.1F3[1]","nurse cells (stage 10), ooplasm (stage 14)",, *F "CyO, P{lArB}A507.2M2","pole cells, brain, posterior spiracles",,"no staining" *F "CyO, P{lArB}A495.1M2","pole cells, head, posterior spiracles","brain, leg disc", *F "CyO, P{lArB}A4.1M2","salivary glands, foregut, hindgut, pharynx, esophagus, posterior spiracles, head, gonads",,"gerarium (region 1), follicle cells (stages 2 or 3-14), squamous follicle cells (stages 9-14)" *F "CyO, P{lArB}A66.2F2","salivary glands, head",,"nurse cells (stage 10), follicle cells (stage 12), squamous follicle cells (stage 12), ovariole sheath muscle" *F "P{lArB}A189.2F3","salivary glands, pharynx, malphighian tubules, CNS subset, posterior spiracles, head, posterior midgut, foregut",,"no staining" *F "P{lArB}C40.1S3","unidentified cells",,"follicle cells, border cells" *F "CyO, P{lArB}A176.1M2","visceral mesoderm (stage 12)",,"no staining" *F "CyO, P{lArB}Fas3[A183.1F2]","visceral mesoderm, clypeolabrum, segment boundary",,"germarium (region 2), border cells, follicle cells" *F "CyO, P{lArB}B39.1M2","yolk",,"no staining" # *U FBrf0103138 *a Hartenstein *b V. *c J.N. *d Jan *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Volker Hartenstein & Juh Nung Jan *F To: Bloomington Drosophila Stock Center *F Background: patterns of lacZ expression *F Information communicated: *F *F "Insertion","Embryo" *F " P{lacW}C6-3-9","CNS" *F " P{lacW}E8-3-63","CNS (stage 10), trachea (stage 11)" *F " P{lacW}C5-2-5","CNS (stage 10), trachea (stage 11), gut (stage 10)" *F " P{lacW}E9-2-2","CNS (stage 11)" *F " P{lacW}l(3)B3-3-21[1]","CNS (stage 11), sensilla (stage 11)" *F " P{lacW}E2-3-27","CNS (stage 11), sensilla (stage 12), gut (stage 11), somatic muscle (muscle pioneers?, stage 12), histoblasts" *F " P{lacW}B8-3-38","CNS (stage 11), sensilla (stage 13), trachea (stage 13), visceral muscle (stage 13), external sensory organ support cells" *F " P{lacW}B1-3-40","CNS (stage 11), visceral muscle (stage 11)" *F " P{lacW}C8-3-5","CNS (stage 12)" *F " P{lacW}A3-3-25","CNS (stage 12), gut" *F " P{lacW}A6-3-2","CNS (stage 12), gut (stage 12), somatic muscle (stage 12)" *F " P{lacW}E10-3-40","CNS (stage 12), sensilla (stage 12)" *F " P{lacW}E6-3-7","CNS (stage 12), sensilla (stage 13), gut, garland cells" *F " P{lacW}E1-2-32","CNS (stage 12), sensilla (stage 13), trachea (stage 12)" *F " P{lacW}B3-2-32","CNS (stage 12), trachea (stage 13), somatic muscle (stage 12), dorsal fat body (stage 12)" *F " P{lacW}B11-3-11","CNS (stage 13)" *F " P{lacW}B12-2-6","CNS (stage 13)" *F " P{lacW}E10-2-41","CNS (stage 13)" *F " P{lacW}E6-3-50","CNS (stage 13)" *F " P{lacW}E10-2-26","CNS (stage 13), amnioserosa" *F " P{lacW}E6-2-17","CNS (stage 13), chordotonal organs?" *F " P{lacW}E9-3-31","CNS (stage 13), gut (stage 15), dorsal vessel (stage 13)" *F " P{lacW}E7-3-33","CNS (stage 13), hemocytes" *F " TM2, P{lacW}l(3)E7-3-58[1]","CNS (stage 13), sensilla (stage 11)" *F " P{lacW}B9-3-40","CNS (stage 13), somatic muscle (stage 11), dorsal vessel (stage 13)" *F " P{lacW}C5-3-22","CNS (stage 13), somatic muscle (stage 12), dorsal vessel (stage 13), hemocytes" *F " P{lacW}E8-3-57","CNS (stage 13), somatic muscle (stage 13), dorsal vessel (stage 13), gonad sheath, dorsal fat body" *F " P{lacW}l(3)B9-3-53[1]","CNS (stage 13), trachea (stage 13), gut (stage 13)" *F " P{lacW}l(3)B4-3-20[1]","CNS (stage 13), triplet cells (unknown structure)" *F " P{lacW}B10-2-13","CNS (stage 14)" *F " P{lacW}C2-2-26","CNS (stage 14)" *F " P{lacW}C6-2-36","CNS (stage 14), gonad sheath (stage 14)" *F " P{lacW}B11-3-6","CNS (stage 14), sensilla (stage 14)" *F " P{lacW}E7-2-17","CNS (stage 14), sensilla (stage 14)" *F " P{lacW}l(3)B7-3-32[1]","CNS (stage 14), sensilla (stage 14)" *F " P{lacW}l(3)B12-3-31[1]","CNS (stage 14), sensilla (stage 14), gonad sheath (stage 15)" *F " P{lacW}A6-3-69","CNS (stage 14), sensilla (stage 14), trachea (stage 14)" *F " P{lacW}B6-2-25","CNS (stage 14), somatic muscle (stage 14), triplet cells (unknown structure, stage 15)" *F " P{lacW}E10-3-33","CNS (stage 14), trachea (stage 13), somatic muscle (stage 13), visceral muscle (stage 13)" *F " P{lacW}l(3)A6-3-56[1]","CNS (stage 15), somatic muscle (stage 15), dorsal vessel (stage 15), oenocytes (stage 15)" *F " P{lacW}C3-2-9","CNS (stage 16), trachea (stage 14), gonads (germ line cells, stage 15)" *F " P{lacW}B9-3-52","CNS (stage 7), sensilla (stage 17), dorsal vessel (stage 13)" *F " P{lacW}E8-2-17","CNS glia (stage 13), sensilla (stage 12)" *F " P{lacW}C8-2-17","CNS glia (stage 13), trachea (stage 12)" *F " P{lacW}C3-2-21","CNS, gut, dorsal vessel, oenocytes" *F " P{lacW}how[E7-3-4]","CNS, somatic muscle (stage 11), visceral muscle (stage 11), dorsal vessel (stage 11), outer sheath of midgut" *F " P{lacW}B7-2-22","CNS, trachea, gut, somatic muscle" *F " P{lacW}mirr[cre2]","epidermis (stage 10), CNS (stage 10), gut (stage 11), oenocytes (stage 12)" *F " P{lacW}A1-2-54","epidermis (stage 10), CNS (stage 10), trachea (stage 10), gut (stage 10)" *F " P{lacW}C6-2-29","epidermis (stage 10), CNS (stage 10), trachea (stage 10), gut (stage 10)" *F " P{lacW}E10-3-1","epidermis (stage 10), CNS (stage 12)" *F " P{lacW}B12-3-5","epidermis (stage 11), CNS (stage 11), gut" *F " P{lacW}A6-3-11","epidermis (stage 11), CNS (stage 13)" *F " P{lacW}A5-2-6","epidermis (stage 11), CNS (stage 13), amnioserosa" *F " P{lacW}E7-3-49","epidermis (stage 11), CNS (stage 13), amnioserosa, triplet cells (unknown structure)" *F " P{lacW}E7-3-63","epidermis (stage 11), CNS (stage 13), dorsal vessel (stage 11), fat body, triplet cells (unknown structure, stage 12)" *F " P{lacW}A4-3-53","epidermis (stage 11), CNS (stage 13), sensilla (stage 11), transverse fiber cells (unknown structure)" *F " P{lacW}E10-2-40","epidermis (stage 11), CNS (stage 13), somatic muscle (?)" *F " P{lacW}A4-3-40","epidermis (stage 11), sensilla (lateral chordotonal organ, stage 12)" *F " P{lacW}l(2)A1-2-12[1]","epidermis (stage 11), sensilla (stage 12), trachea (stage 11)" *F " P{lacW}C8-3-37","epidermis (stage 11), trachea, degenerating cells (stage 12)" *F " P{lacW}A3-2-66","epidermis (stage 12), CNS (stage 12), visceral muscle (stage 13)" *F " P{lacW}C5-2-7","epidermis (stage 12), CNS (stage 13), gut (stage 15), visceral muscle (stage 13)" *F " P{lacW}C4-3-20","epidermis (stage 12), CNS (stage 13), sensilla (stage 13), gonad sheath" *F " P{lacW}E7-3-52","epidermis (stage 12), CNS (stage 13), triplet cells (unknown structure), imaginal discs" *F " P{lacW}E1-2-38","epidermis (stage 12), CNS (stage 13), visceral muscle (stage 12)" *F " P{lacW}A6-2-45","epidermis (stage 12), CNS (stage 9)" *F " P{lacW}A3-3-61","epidermis (stage 12), CNS, trachea (stage 12), gut (stage 12), visceral muscle (stage 12), dorsal vessel (stage 13)" *F " P{lacW}A1-3-10","epidermis (stage 12), trachea (stage 11), gut" *F " P{lacW}C3-3-30","epidermis (stage 13), CNS (stage 13), gut (stage 13)" *F " P{lacW}B1-3-49","epidermis (stage 13), CNS (stage 13), gut (stage 13), visceral muscle (stage 13), dorsal fat body (stage 15)" *F " P{lacW}C3-2-2","epidermis (stage 13), gut, visceral muscle, oenocytes" *F " P{lacW}B4-3-8","epidermis (stage 13), sensilla (stage 16)" *F " P{lacW}A3-3-47","epidermis (stage 13), trachea (stage 13), gut (stage 13)" *F " P{lacW}B4-3-31","epidermis (stage 14), sensilla (stage 14), trachea (stage 14), gut (stage 14)" *F " P{lacW}l(3)E8-3-54[1]","epidermis (stage 15), trachea (stage 13), gut (stage 15)" *F " P{lacW}B4-2-27","epidermis (stage 15), trachea (stage 15), somatic muscle (stage 14), oenocytes (stage 12)" *F " P{lacW}D3-3-11","epidermis (stage 7), CNS (stage 9), visceral muscle (stage 13)" *F " P{lacW}A1-3-1","epidermis (stage 9), CNS (stage 8), gut (stage 3), visceral muscle (stage 11)" *F " P{lacW}C7-3-34","epidermis, CNS (stage 14)" *F " P{lacW}B10-2-3","gonad sheath (stage 15)" *F " P{lacW}E8-2-18","hemocytes (stage 12)" *F " P{lacW}B9-3-25","sensilla (stage 13), gut (stage 9), somatic muscle (stage 9), visceral muscle (stage 9), dorsal vessel (stage 12)" *F " P{lacW}B6-2-30","somatic muscle (stage 12), visceral muscle (stage 12)" *F " P{lacW}A4-2-5","somatic muscle (stage 13)" *F " P{lacW}B6-3-23","somatic muscle (stage 13)" *F " P{lacW}B9-2-33","somatic muscle (stage 14)" *F " P{lacW}C3-3-36","trachea (stage 13), gonad sheath (stage 13)" *F " P{lacW}B6-3-18","visceral muscle (stage 11), gonad sheath" # *U FBrf0103139 *a Klambt *b C. *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Christian Klambt *F To: Bloomington Drosophila Stock Center *F Background: patterns of lacZ expression *F Information communicated: *F *F "Insertion","Embryo" *F "P{PZ}rC56","glial cells" *F "P{lwB}AA142","midline glial cells" # *U FBrf0103140 *a Hafen *b E. *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Ernst Hafen *F To: Bloomington Drosophila Stock Center *F Background: patterns of lacZ expression *F Information communicated: *F *F "Insertion","Larva" *F "P{lArB}47","eye disc, band behind morphogenetic furrow" *F "P{lArB}99","eye disc, band behind morphogenetic furrow" *F "P{lArB}166","eye disc, band behind the morphogenetic furrow" *F "P{lArB}175","eye disc, band behind the morphogenetic furrow" *F "P{lArB}112","eye disc, behind the morphogenetic furrow" *F "P{lArB}154","eye disc, behind the morphogenetic furrow" *F "P{lArB}103","eye disc, clusters behind the morphogenetic furrow" *F "P{lArB}58","eye disc, clusters behind the morphogenetic furrow" *F "P{lArB}195","eye disc, stronger staining behind than ahead of the morphogenetic furrow" # *U FBrf0103141 *a Blair *b S. *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Seth Blair *F To: Bloomington Drosophila Stock Center *F Background: patterns of lacZ expression *F Information communicated: *F *F "Insertion","Pupa" *F "P{lArB}A229.1F1","uniform" # *U FBrf0103142 *a Vosshall *b L. *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Leslie Vosshall *F To: Bloomington Drosophila Stock Center *F Background: patterns of lacZ expression *F Information communicated: *F *F "Insertion","Adult Brain" *F "P{PZ}l(3)08126[08126]"," central brain" *F "P{PZ}l(3)00720[00720]"," faint central brain" *F "P{PZ}l(3)05146[05146]"," fat body" *F "P{PZ}Atpalpha[00295]","3rd antennal segment" *F "P{PZ}cpo[01432]","3rd antennal segment" *F "P{PZ}l(2)08323[08323]","3rd antennal segment" *F "P{PZ}l(2)10523[10523]","3rd antennal segment" *F "P{PZ}l(3)00274[00274]","3rd antennal segment" *F "P{PZ}l(3)00506[00506]","3rd antennal segment" *F "P{PZ}l(3)00643[00643]","3rd antennal segment" *F "P{PZ}l(3)01319[01319]","3rd antennal segment" *F "P{PZ}l(3)01344[01344]","3rd antennal segment" *F "P{PZ}l(3)01688[01688]","3rd antennal segment" *F "P{PZ}l(3)02240[02240]","3rd antennal segment" *F "P{PZ}l(3)02248[02248]","3rd antennal segment" *F "P{PZ}l(3)02515[02515]","3rd antennal segment" *F "P{PZ}l(3)02937[02937]","3rd antennal segment" *F "P{PZ}l(3)03692[03692]","3rd antennal segment" *F "P{PZ}l(3)07621[07621]","3rd antennal segment" *F "P{PZ}l(3)10585[10585]","3rd antennal segment" *F " P{lacW}C3-2-2","3rd antennal segment, central brain" *F "P{PZ}l(3)05652[05652]","3rd antennal segment, dorsal central brain" *F "P{PZ}klu[10052]","3rd antennal segment, faint central brain" *F "P{PZ}l(2)03264[03264]","3rd antennal segment, faint central brain" *F "P{PZ}l(3)01814[01814]","3rd antennal segment, fat body" *F "P{PZ}l(2)00329[00329]","R1-6, optic lobe" *F "P{PZ}l(3)01470[01470]","R1-6, optic lobe, central brain" *F "P{PZ}l(2)01855[01855]","R1-8" *F "P{PZ}crp[00232]","R1-8, 3rd antennal segment" *F "P{PZ}l(2)05315[05315]","R1-8, dorsal cells at retina/lamina border" *F "P{PZ}l(2)10642[10642]","R1-8, lamina, fat body, 3rd antennal segment" *F "P{PZ}l(3)00707[00707]","R1-8, optic lobe, central brain" *F "P{PZ}l(3)01318[01318]","R1-8, optic lobe, central brain" *F "P{PZ}sm[05338]","R8" *F "P{PZ}gil[05845]","antennal lobe" *F "P{PZ}l(3)neo22[02281]","antennal lobe, 3rd antennal segment" *F "P{PZ}al[05142]","arista" *F "P{PZ}l(3)09904[09904]","arista, 3rd antennal segment" *F " P{lacW}A1-3-10","central brain" *F "P{PZ}Dl[05151]","central brain" *F "P{PZ}Ten-m[05309]","central brain" *F "P{PZ}l(2)00297[00297]","central brain" *F "P{PZ}l(2)01085[01085]","central brain" *F "P{PZ}l(2)01351[01351]","central brain" *F "P{PZ}l(2)01424[01424]","central brain" *F "P{PZ}l(2)03263[03263]","central brain" *F "P{PZ}l(2)03427[03427],","central brain" *F "P{PZ}l(2)03497[03497]","central brain" *F "P{PZ}l(2)03531[03531]","central brain" *F "P{PZ}l(2)03563[03563]","central brain" *F "P{PZ}l(2)04535[04535]","central brain" *F "P{PZ}l(2)05056[05056]","central brain" *F "P{PZ}l(2)09373[09373]","central brain" *F "P{PZ}l(3)00714[00714]","central brain" *F "P{PZ}l(3)02231[02231]","central brain" *F "P{PZ}l(3)03077[03077]","central brain" *F "P{PZ}l(3)03559[03559]","central brain" *F "P{PZ}l(3)05279[05279]","central brain" *F "P{PZ}l(3)05697[05697]","central brain" *F "P{PZ}l(3)05712[05712]","central brain" *F "P{PZ}l(3)06240[06240]","central brain" *F "P{PZ}lola[00642]","central brain" *F "P{PZ}lwr[05486]","central brain" *F "P{PZ}syx1A[06737]","central brain" *F "P{PZ}ttk[02667]","central brain" *F "P{PZ}Gsc[05341]","central brain (dorsal)" *F "P{PZ}l(2)08492[08492]","central brain , fat body" *F "P{PZ}hth[05745]","central brain, 3rd antennal segment" *F "P{PZ}l(2)06373[06373]","central brain, 3rd antennal segment" *F "P{PZ}l(2)07768[07768]","central brain, 3rd antennal segment" *F "P{PZ}l(3)09291[09291]","central brain, 3rd antennal segment" *F "P{PZ}l(3)10477[10477]","central brain, 3rd antennal segment" *F "P{PZ}sema-II[03021]","central brain, 3rd antennal segment" *F "P{PZ}tws[02414]","central brain, 3rd antennal segment" *F "P{PZ}l(3)01949[01949]","central brain, 3rd antennal segment," *F "P{PZ}l(3)00716[00716]","central brain, antennal lobe" *F "P{PZ}l(3)01453[01453]","central brain, antennal lobe" *F "P{PZ}l(3)09036[09036]","central brain, antennal lobe, 3rd antennal segment" *F "P{PZ}14-3-3[07103]","central brain, antennal lobe, mushroom body" *F "P{PZ}l(2)02321[02321]","central brain, bilateral" *F "P{PZ}Dll[01092]","central brain, fat body" *F "P{PZ}l(3)00451[00451]","central brain, fat body" *F "P{PZ}l(3)02521[02521]","central brain, fat body" *F "P{PZ}l(3)05137[05137]","central brain, fat body" *F "P{PZ}l(3)06536[06536]","central brain, fat body" *F "P{PZ}l(2)10481[10481]","central brain, mushroom body" *F "P{PZ}l(3)06490[06490]","central brain, mushroom body, antennal lobe" *F "P{PZ}mod(mdg4)[03852]","central brain, mushroom body, antennal lobe" *F "P{PZ}l(2)00681[00681]","central brain, optic lobe" *F "P{PZ}l(2)01848[01848]","central brain, optic lobe" *F "P{PZ}l(3)05884[05884]","central brain, optic lobe" *F "P{PZ}pros[10419]","central brain, optic lobe (lamina cartridges)" *F "P{PZ}l(2)06243[06243]","central brain, optic lobe, 3rd antennal segment" *F "P{PZ}l(3)09070[09070]","central brain, optic lobe, antennal lobe" *F "P{PZ}l(2)01466[01466]","central brain,ocelli, R1-8" *F "P{PZ}trx[00347]","cluster of cells in optic lobe" *F "P{PZ}Cam[03909]","discrete central brain" *F "P{PZ}kis[07812]","discrete central brain" *F "P{PZ}l(2)00629[00629]","discrete central brain" *F "P{PZ}l(2)01103[01103]","discrete central brain" *F "P{PZ}l(2)02637[02637]","discrete central brain" *F "P{PZ}l(2)04845[04845]","discrete central brain" *F "P{PZ}l(2)05836[05836]","discrete central brain" *F "P{PZ}l(2)10444[10444]","discrete central brain" *F "P{PZ}l(3)00073[00073]","discrete central brain" *F "P{PZ}l(3)09656[09656]","discrete central brain" *F "P{PZ}l(2)01810[01810]","discrete central brain and optic lobe" *F " P{lacW}C8-3-5","discrete central brain, 3rd antennal segment" *F "P{PZ}l(3)03699[03699]","discrete central brain, 3rd antennal segment" *F " P{lacW}C3-3-30","discrete optic lobe" *F "P{PZ}l(2)03996[03996]","discrete optic lobe and central brain" *F "P{PZ}l(3)02733[02733]","discrete optic lobe and central brain" *F "P{PZ}Hrb27C[02647]","discrete, faint central brain" *F "P{PZ}drk[10626]","discrete, faint central brain and optic lobe" *F " P{lacW}C7-3-34","eye, optic lobe, central brain" *F "P{PZ}sr[03999]","faint 3rd antennal segment and 2nd antennal segment" *F "P{PZ}l(3)06664[06664]","faint antennal lobe" *F "P{PZ}l(2)10521[10521]","faint central brain" *F "P{PZ}l(3)04026[04026]","faint central brain" *F "P{PZ}l(3)07217[07217]","faint central brain" *F "P{PZ}l(3)07238[07238]","faint central brain" *F "P{PZ}l(3)03022[03022]","faint central brain, 3rd antennal segment" *F "P{PZ}l(3)03076[03076]","faint central brain, 3rd antennal segment" *F "P{PZ}l(3)07615[07615]","faint central brain, optic lobe" *F "P{PZ}l(2)02502[02502]","faint global" *F "P{PZ}l(3)01658[01658]","faint lamina and central brain" *F "P{PZ}l(3)01673[01673]","faint lamina and central brain" *F "P{PZ}l(3)03928[03928]","faint ocelli" *F "P{PZ}l(3)05871[05871]","faint ocelli" *F "P{PZ}fray[07551]","faint, discrete central brain" *F "P{PZ}l(2)02970[02970]","faint, discrete central brain" *F " P{lacW}E10-2-40","fat body" *F "P{PZ}Stat92E[06346]","fat body" *F "P{PZ}l(2)01289[01289]","fat body" *F "P{PZ}l(2)01482[01482]","fat body" *F "P{PZ}l(2)06250[06250]","fat body" *F "P{PZ}l(3)02094[02094]","fat body" *F "P{PZ}l(3)05088[05088]","fat body" *F "P{PZ}l(3)06677[06677]","fat body" *F "P{PZ}neb[03552]","fat body" *F "P{PZ}l(2)05847[05847]","fat body, 3rd antennal segment" *F " P{lacW}B9-3-25","fat body, optic lobe" *F "P{PZ}l(3)05203[05203]","fat body, optic lobe" *F "P{PZ}Pka-C1[01272]","global" *F "P{PZ}l(2)00632[00632]","global" *F "P{PZ}l(2)01270[01270]","global" *F "P{PZ}l(2)03350[03350]","global" *F "P{PZ}l(2)10608[10608]","global" *F "P{PZ}scat[03550]","global" *F "P{PZ}Ef1alpha48D[01275]","global central brain" *F "P{PZ}Hsr93D[05241]","global central brain" *F "P{PZ}l(2)03105[03105]","global central brain" *F "P{PZ}l(2)05337[05337]","global central brain" *F "P{PZ}l(3)06439[06439]","global central brain" *F "P{PZ}trh[10512]","global central brain" *F "P{PZ}l(2)08269[08269]","global central brain and optic lobe" *F "P{PZ}spi[01068]","global central brain and optic lobe" *F "P{PZ}l(3)06142[06142]","global central brain, 3rd AS" *F "P{PZ}l(2)05351[05351]","global expression in central brain, optic lobe" *F "P{PZ}svp[07842]","global in optic lobe, fat body, 3rd antennal segment" *F "P{PZ}l(3)03463[03463]","global, fat body" *F "P{PZ}RhoGEF2[04291]","lamina" *F "P{PZ}guf[02833]","lamina" *F "P{PZ}l(2)00734[00734]","lamina" *F "P{PZ}l(2)01296[01296]","lamina" *F "P{PZ}l(3)05113[05113]","lamina" *F "P{PZ}pnt[07825]","lamina" *F "P{PZ}stg[01235]","lamina" *F "P{PZ}wg[02657]","lamina" *F " P{lacW}B9-3-40","lamina, 3rd antennal segment" *F "P{PZ}l(3)02640[02640]","lamina, 3rd antennal segment" *F "P{PZ}l(3)78Cb[00217]","lamina, 3rd antennal segment" *F "P{PZ}shg[10469]","lamina, 3rd antennal segment" *F "P{PZ}l(2)04440[04440]","lamina, 3rd antennal segment, antennal lobe" *F "P{PZ}l(3)05822[05822]","lamina, antennal lobe" *F "P{PZ}l(2)00231[00231]","lamina, central brain" *F "P{PZ}l(2)02353[02353]","lamina, central brain" *F "P{PZ}l(3)06658[06658]","lamina, central brain" *F "P{PZ}l(3)neo42[02404]","lamina, central brain" *F "P{PZ}eff[01462]","lamina, central brain, 3rd antennal segment" *F " P{lacW}E8-2-17","lamina, discrete central brain" *F "P{PZ}l(3)03773[03773]","lamina, fat body" *F "P{PZ}l(2)01094[01094]","lamina, medulla" *F "P{PZ}18w[00053]","lamina, medulla, 3rd antennal segment" *F "P{PZ}eld[00090]","lamina, medulla, central brain" *F "P{PZ}l(3)08232[08232]","lamina, medulla, central brain, 3rd antennal segment" *F "P{PZ}l(2)01857[01857]","maxillary palp" *F " P{lacW}C2-2-26","medulla" *F "P{PZ}l(3)00281[00281]","medulla" *F " P{lacW}E6-3-7","medulla, central brain" *F "P{PZ}l(2)03972[03972]","mushroom body, antennal lobe" *F " P{lacW}A1-2-54","no staining" *F " P{lacW}A1-3-1","no staining" *F " P{lacW}A3-2-66","no staining" *F " P{lacW}B10-2-13","no staining" *F " P{lacW}B10-2-3","no staining" *F " P{lacW}B11-3-11","no staining" *F " P{lacW}B11-3-6","no staining" *F " P{lacW}B12-2-6","no staining" *F " P{lacW}B12-3-5","no staining" *F " P{lacW}B9-3-52","no staining" *F " P{lacW}C3-2-21","no staining" *F " P{lacW}C3-2-9","no staining" *F " P{lacW}C3-3-36","no staining" *F " P{lacW}C5-2-5","no staining" *F " P{lacW}C5-2-7","no staining" *F " P{lacW}C5-3-22","no staining" *F " P{lacW}C6-2-36","no staining" *F " P{lacW}C8-3-37","no staining" *F " P{lacW}D3-3-11","no staining" *F " P{lacW}E1-2-38","no staining" *F " P{lacW}E10-2-26","no staining" *F " P{lacW}E10-2-41","no staining" *F " P{lacW}E10-3-1","no staining" *F " P{lacW}E10-3-33","no staining" *F " P{lacW}E10-3-40","no staining" *F " P{lacW}E2-3-27","no staining" *F " P{lacW}E6-2-17","no staining" *F " P{lacW}E6-3-50","no staining" *F " P{lacW}E7-2-17","no staining" *F " P{lacW}E7-3-33","no staining" *F " P{lacW}E7-3-49","no staining" *F " P{lacW}E7-3-63","no staining" *F " P{lacW}E8-2-18","no staining" *F " P{lacW}E8-3-63","no staining" *F " P{lacW}E9-2-2","no staining" *F " P{lacW}E9-3-31","no staining" *F " P{lacW}l(2)A1-2-12[1]","no staining" *F " P{lacW}l(3)B9-3-53[1]","no staining" *F " P{lacW}l(3)E8-3-54[1]","no staining" *F " TM2, P{lacW}l(3)E7-3-58[1]","no staining" *F "P{PZ}CycA[03946]","no staining" *F "P{PZ}CycE[05206]","no staining" *F "P{PZ}Dcp-1[01862]","no staining" *F "P{PZ}Dcp-1[02132]","no staining" *F "P{PZ}Doa[01705]","no staining" *F "P{PZ}E2f[07172]","no staining" *F "P{PZ}Hem[03335]","no staining" *F "P{PZ}Itp-r83A[05616]","no staining" *F "P{PZ}Klp61F[07012]","no staining" *F "P{PZ}Kr[00895]","no staining" *F "P{PZ}Tm1[02299]","no staining" *F "P{PZ}W[05014]","no staining" *F "P{PZ}abs[00620]","no staining" *F "P{PZ}alpha-Adaptin[06694]","no staining" *F "P{PZ}apt[09049]","no staining" *F "P{PZ}bnl[06916]","no staining" *F "P{PZ}bun[00255]","no staining" *F "P{PZ}chn[02064]","no staining" *F "P{PZ}ck[07130]","no staining" *F "P{PZ}cnc[03921]","no staining" *F "P{PZ}dap[04454]","no staining" *F "P{PZ}dev[00208]","no staining" *F "P{PZ}dpp[10638]","no staining" *F "P{PZ}fs(3)07084[07084]","no staining" *F "P{PZ}ftz-f1[03649]","no staining" *F "P{PZ}gsb-d[01155]","no staining" *F "P{PZ}kuz[03782]","no staining" *F "P{PZ}l(2)00064[00064]","no staining" *F "P{PZ}l(2)00248[00248]","no staining" *F "P{PZ}l(2)00572[00572]","no staining" *F "P{PZ}l(2)00628[00628]","no staining" *F "P{PZ}l(2)01038[01038]","no staining" *F "P{PZ}l(2)01265[01265]","no staining" *F "P{PZ}l(2)01288[01288]","no staining" *F "P{PZ}l(2)01349[01349]","no staining" *F "P{PZ}l(2)01361[01361]","no staining" *F "P{PZ}l(2)01410[01410]","no staining" *F "P{PZ}l(2)01433[01433]","no staining" *F "P{PZ}l(2)01501[01501]","no staining" *F "P{PZ}l(2)01738[01738]","no staining" *F "P{PZ}l(2)01863[01863]","no staining" *F "P{PZ}l(2)02029[02029]","no staining" *F "P{PZ}l(2)02045[02045]","no staining" *F "P{PZ}l(2)02047[02047]","no staining" *F "P{PZ}l(2)02074[02074]","no staining" *F "P{PZ}l(2)02107[02107]","no staining" *F "P{PZ}l(2)02306[02306]","no staining" *F "P{PZ}l(2)02448[02448]","no staining" *F "P{PZ}l(2)02535[02535]","no staining" *F "P{PZ}l(2)02695[02695]","no staining" *F "P{PZ}l(2)02836[02836]","no staining" *F "P{PZ}l(2)02858[02858]","no staining" *F "P{PZ}l(2)03050[03050]","no staining" *F "P{PZ}l(2)03068[03068]","no staining" *F "P{PZ}l(2)03091[03091]","no staining" *F "P{PZ}l(2)03221[03221]","no staining" *F "P{PZ}l(2)03235[03235]","no staining" *F "P{PZ}l(2)03405[03405]","no staining" *F "P{PZ}l(2)03605[03605]","no staining" *F "P{PZ}l(2)03610[03610]","no staining" *F "P{PZ}l(2)03659[03659]","no staining" *F "P{PZ}l(2)03697[03697]","no staining" *F "P{PZ}l(2)03709[03709]","no staining" *F "P{PZ}l(2)03728[03728]","no staining" *F "P{PZ}l(2)03771[03771]","no staining" *F "P{PZ}l(2)03775[03775]","no staining" *F "P{PZ}l(2)03788[03788]","no staining" *F "P{PZ}l(2)03832[03832]","no staining" *F "P{PZ}l(2)03845[03845]","no staining" *F "P{PZ}l(2)03953[03953]","no staining" *F "P{PZ}l(2)04008[04008]","no staining" *F "P{PZ}l(2)04012[04012]","no staining" *F "P{PZ}l(2)04065[04065]","no staining" *F "P{PZ}l(2)04154[04154]","no staining" *F "P{PZ}l(2)04227[04227]","no staining" *F "P{PZ}l(2)04329[04329]","no staining" *F "P{PZ}l(2)04418[04418]","no staining" *F "P{PZ}l(2)04431[04431]","no staining" *F "P{PZ}l(2)04493[04493]","no staining" *F "P{PZ}l(2)04524[04524]","no staining" *F "P{PZ}l(2)04530[04530]","no staining" *F "P{PZ}l(2)04643[04643]","no staining" *F "P{PZ}l(2)04820[04820]","no staining" *F "P{PZ}l(2)04884[04884]","no staining" *F "P{PZ}l(2)05006[05006]","no staining" *F "P{PZ}l(2)05070[05070]","no staining" *F "P{PZ}l(2)05091[05091]","no staining" *F "P{PZ}l(2)05287[05287]","no staining" *F "P{PZ}l(2)05327[05327]","no staining" *F "P{PZ}l(2)05488[05488]","no staining" *F "P{PZ}l(2)05642[05642]","no staining" *F "P{PZ}l(2)05643[05643],","no staining" *F "P{PZ}l(2)05714[05714]","no staining" *F "P{PZ}l(2)06214[06214]","no staining" *F "P{PZ}l(2)06225[06225]","no staining" *F "P{PZ}l(2)06253[06253]","no staining" *F "P{PZ}l(2)06270[06270]","no staining" *F "P{PZ}l(2)06320[06320]","no staining" *F "P{PZ}l(2)06339[06339]","no staining" *F "P{PZ}l(2)06369[06369]","no staining" *F "P{PZ}l(2)06424[06424]","no staining" *F "P{PZ}l(2)06444[06444]","no staining" *F "P{PZ}l(2)06496[06496]","no staining" *F "P{PZ}l(2)06708[06708]","no staining" *F "P{PZ}l(2)06736[06736]","no staining" *F "P{PZ}l(2)06825[06825]","no staining" *F "P{PZ}l(2)06860[06860]","no staining" *F "P{PZ}l(2)06949[06949]","no staining" *F "P{PZ}l(2)07022[07022]","no staining" *F "P{PZ}l(2)07054[07054]","no staining" *F "P{PZ}l(2)07129[07129]","no staining" *F "P{PZ}l(2)07206[07206]","no staining" *F "P{PZ}l(2)07214[07214]","no staining" *F "P{PZ}l(2)07806[07806]","no staining" *F "P{PZ}l(2)07837[07837]","no staining" *F "P{PZ}l(2)07838[07838]","no staining" *F "P{PZ}l(2)08014[08014]","no staining" *F "P{PZ}l(2)08307[08307]","no staining" *F "P{PZ}l(2)08685[08685]","no staining" *F "P{PZ}l(2)08717[08717]","no staining" *F "P{PZ}l(2)08770[08770]","no staining" *F "P{PZ}l(2)08774[08774]","no staining" *F "P{PZ}l(2)09639[09639]","no staining" *F "P{PZ}l(2)10424[10424]","no staining" *F "P{PZ}l(2)10425[10425]","no staining" *F "P{PZ}l(2)10505[10505]","no staining" *F "P{PZ}l(2)10685[10685]","no staining" *F "P{PZ}l(2)35Bd[10408]","no staining" *F "P{PZ}l(2)43Bb[04614]","no staining" *F "P{PZ}l(2)43Bc[05518]","no staining" *F "P{PZ}l(3)00035[00035]","no staining" *F "P{PZ}l(3)00082[00082]","no staining" *F "P{PZ}l(3)00096[00096]","no staining" *F "P{PZ}l(3)00534[00534]","no staining" *F "P{PZ}l(3)00543[00543]","no staining" *F "P{PZ}l(3)00564[00564]","no staining" *F "P{PZ}l(3)00827[00827]","no staining" *F "P{PZ}l(3)00835[00835]","no staining" *F "P{PZ}l(3)00864[00864]","no staining" *F "P{PZ}l(3)01029[01029]","no staining" *F "P{PZ}l(3)01086[01086]","no staining" *F "P{PZ}l(3)01107[01107]","no staining" *F "P{PZ}l(3)01152[01152]","no staining" *F "P{PZ}l(3)01207[01207]","no staining" *F "P{PZ}l(3)01239[01239]","no staining" *F "P{PZ}l(3)01456[01456]","no staining" *F "P{PZ}l(3)01618[01618]","no staining" *F "P{PZ}l(3)01640[01640]","no staining" *F "P{PZ}l(3)01969[01969]","no staining" *F "P{PZ}l(3)02069[02069]","no staining" *F "P{PZ}l(3)02102[02102]","no staining" *F "P{PZ}l(3)02255[02255]","no staining" *F "P{PZ}l(3)02267[02267]","no staining" *F "P{PZ}l(3)02331[02331]","no staining" *F "P{PZ}l(3)02540[02540]","no staining" *F "P{PZ}l(3)02619[02619]","no staining" *F "P{PZ}l(3)02732[02732]","no staining" *F "P{PZ}l(3)03477[03477]","no staining" *F "P{PZ}l(3)03644[03644]","no staining" *F "P{PZ}l(3)03670[03670]","no staining" *F "P{PZ}l(3)03675[03675]","no staining" *F "P{PZ}l(3)03834[03834]","no staining" *F "P{PZ}l(3)03844[03844]","no staining" *F "P{PZ}l(3)03847[03847]","no staining" *F "P{PZ}l(3)03881[03881]","no staining" *F "P{PZ}l(3)03988[03988]","no staining" *F "P{PZ}l(3)04053[04053]","no staining" *F "P{PZ}l(3)04063[04063]","no staining" *F "P{PZ}l(3)04091[04091]","no staining" *F "P{PZ}l(3)04093[04093]","no staining" *F "P{PZ}l(3)04210[04210]","no staining" *F "P{PZ}l(3)04220[04220]","no staining" *F "P{PZ}l(3)04226[04226]","no staining" *F "P{PZ}l(3)04281[04281]","no staining" *F "P{PZ}l(3)04449[04449]","no staining" *F "P{PZ}l(3)04498[04498]","no staining" *F "P{PZ}l(3)04521[04521]","no staining" *F "P{PZ}l(3)04556[04556]","no staining" *F "P{PZ}l(3)04629[04629]","no staining" *F "P{PZ}l(3)04684[04684]","no staining" *F "P{PZ}l(3)04696[04696]","no staining" *F "P{PZ}l(3)04708[04708]","no staining" *F "P{PZ}l(3)04713[04713]","no staining" *F "P{PZ}l(3)04837[04837]","no staining" *F "P{PZ}l(3)05043[05043]","no staining" *F "P{PZ}l(3)05057[05057]","no staining" *F "P{PZ}l(3)05089[05089]","no staining" *F "P{PZ}l(3)05408[05408]","no staining" *F "P{PZ}l(3)05461[05461]","no staining" *F "P{PZ}l(3)05592[05592]","no staining" *F "P{PZ}l(3)05634[05634]","no staining" *F "P{PZ}l(3)05820[05820]","no staining" *F "P{PZ}l(3)05967[05967]","no staining" *F "P{PZ}l(3)06404[06404]","no staining" *F "P{PZ}l(3)06442[06442]","no staining" *F "P{PZ}l(3)06465[06465]","no staining" *F "P{PZ}l(3)06487[06487]","no staining" *F "P{PZ}l(3)06497[06497]","no staining" *F "P{PZ}l(3)06524[06524]","no staining" *F "P{PZ}l(3)06713[06713]","no staining" *F "P{PZ}l(3)06803[06803]","no staining" *F "P{PZ}l(3)06811[06811]","no staining" *F "P{PZ}l(3)06906[06906]","no staining" *F "P{PZ}l(3)06924[06924]","no staining" *F "P{PZ}l(3)06945[06945]","no staining" *F "P{PZ}l(3)07013[07013]","no staining" *F "P{PZ}l(3)07086[07086]","no staining" *F "P{PZ}l(3)07882[07882]","no staining" *F "P{PZ}l(3)08310[08310]","no staining" *F "P{PZ}l(3)10112[10112]","no staining" *F "P{PZ}l(3)10418[10418]","no staining" *F "P{PZ}l(3)10547[10547]","no staining" *F "P{PZ}l(3)10621[10621]","no staining" *F "P{PZ}l(3)10631[10631]","no staining" *F "P{PZ}l(3)70Da[02402]","no staining" *F "P{PZ}l(3)72Dd[03802]","no staining" *F "P{PZ}l(3)neo25[07041]","no staining" *F "P{PZ}lab[01241]","no staining" *F "P{PZ}lmg[03424]","no staining" *F "P{PZ}mam[03505]","no staining" *F "P{PZ}mam[04615]","no staining" *F "P{PZ}mts[02496]","no staining" *F "P{PZ}nos[07117]","no staining" *F "P{PZ}oho23B[03575]","no staining" *F "P{PZ}put[10460]","no staining" *F "P{PZ}salm[03602]","no staining" *F "P{PZ}sgl[05007]","no staining" *F "P{PZ}shn[04738]","no staining" *F "P{PZ}sprd[05284]","no staining" *F "P{PZ}srp[01549]","no staining" *F "P{PZ}tdf[03041]","no staining" *F "P{PZ}tkv[04415]","no staining" *F "P{PZ}tsh[04319]","no staining" *F "P{PZ}twr[05614]","no staining" *F "P{PZ}vkg[01209]","no staining" *F "P{PZ}vn[10567]","no staining" *F "P{PZ}wb[09437]","no staining" *F "P{PZ}zfh1[00865]","no staining" *F "P{PZ}zip[02957]","no staining" *F "P{lacW}B8-3-38","no staining" *F "P{PZ}l(3)07207[07207]","ocelli" *F "P{PZ}tws[01436]","ocelli" *F "P{PZ}l(3)08724[08724]","ocelli, medulla" *F "P{PZ}l(3)05430[05430]","ocelli, central brain" *F "P{PZ}slp1[05965]","ocelli, lamina" *F "P{PZ}l(2)01467[01467]","ocelli, medulla, lamina" *F " P{lacW}C4-3-20","optic lobe" *F " P{lacW}E7-3-52","optic lobe" *F " P{lacW}E8-3-57","optic lobe" *F "P{PZ}Vha68-2[01510]","optic lobe" *F "P{PZ}emc[04322]","optic lobe" *F "P{PZ}fam[05475]","optic lobe" *F "P{PZ}l(2)10491[10491]","optic lobe" *F "P{PZ}l(3)00836[00836]","optic lobe" *F "P{PZ}l(3)00848[00848]","optic lobe" *F "P{PZ}l(3)01629[01629]","optic lobe" *F "P{PZ}l(3)09143[09143]","optic lobe" *F " P{lacW}C6-2-29","optic lobe and brain, 2nd and 3rd antennal segments" *F "P{PZ}bs[03267]","optic lobe, 2nd antennal segment" *F "P{PZ}l(3)01544[01544]","optic lobe, 3rd antennal segment, fat body" *F "P{PZ}InR[05545]","optic lobe, central brain" *F "P{PZ}Rca1[03300]","optic lobe, central brain" *F "P{PZ}dally[06464]","optic lobe, central brain" *F "P{PZ}l(2)02516[02516]","optic lobe, central brain" *F "P{PZ}l(2)07659[07659]","optic lobe, central brain" *F "P{PZ}l(3)06743[06743]","optic lobe, central brain" *F "P{PZ}l(3)07128[07128]","optic lobe, central brain" *F "P{PZ}l(3)09645[09645]","optic lobe, central brain" *F "P{PZ}msn[06946]","optic lobe, central brain" *F " P{lacW}C6-3-9","optic lobe, central brain, 2nd antennal segment" *F " P{lacW}E1-2-32","optic lobe, central brain, 2nd antennal segment" *F " P{lacW}C8-2-17","optic lobe, central brain, 3rd antennal segment" *F "P{PZ}h[08247]","optic lobe, central brain, 3rd antennal segment" *F "P{PZ}l(3)03342[03342]","optic lobe, central brain, 3rd antennal segment" *F "P{PZ}repo[03702]","optic lobe, central brain, 3rd antennal segment" *F "P{PZ}l(3)03429[03429]","optic lobe, central brain, antennal lobe" *F "P{PZ}l(3)06951[06951]","optic lobe, central brain, fat body, 3rd antennal lobe" *F "P{PZ}l(3)04069[04069]","optic lobe, faint central brain, 3rd antennal segment" *F " P{lacW}how[E7-3-4]","proboscis" *F "P{PZ}stc[05441]","proboscis" *F "P{PZ}l(2)05428[05428]","proboscis, fat body" *F " P{lacW}l(3)B12-3-31[1]","sparse optic lobe, 3rd antennal segment" *F "P{PZ}03349[03349]","weak central brain, antennal lobe" # *U FBrf0103143 *a Jackle *b H. *c W. *d Janning *t 1998.8.10 *T personal communication to FlyBase *u *F Personnal communication from: Herbert Jackle & Wilfried Janning *F To: Bloomington Drosophila Stock Center *F Background: patterns of lacZ expression *F Information communicated: *F *F "Insertion","Embryo" *F "P{PZ}l(2)01094[01094]"," CNS (pattern), foregut, hindgut, larval brain hemisphere, mesoderm, midgut, PNS, posterior midgut, segmental pattern, stomodeum" *F "P{PZ}Dll[01092]"," CNS (pattern), head, imaginal discs (?), PNS, segmental pattern, stripe anterior to cephalic furrow, thoracic segments (?)" *F "P{PZ}l(2)00632[00632]"," CNS (pattern), hindgut, larval brain hemisphere, lethal phenotype, mesoderm, midgut, PNS, segmental pattern, somatic muscles, stomodeum" *F "P{PZ}l(2)00681[00681]"," CNS (pattern), pharynx (PNS ?), PNS, segmental pattern (PNS)" *F "P{PZ}l(2)00734[00734]"," CNS, head, hindgut, larval brain hemisphere, mesoderm, pharynx, PNS (?), posterior midgut, proventriculus, segmental pattern (PNS ?), stomodeum" *F "P{PZ}slp1[05965]","14-15 stripes (segmental pattern), CNS (pattern), dorsal vessel (?), larval brain hemisphere, segmental pattern, stripe anterior to cephalic furrow" *F "P{PZ}lmg[03424]","3 stripes, CNS (pattern), larval brain hemisphere, mesoderm (weak), midgut, PNS (pattern), segmental pattern, stomodeal plate" *F "P{PZ}spi[01068]","CNS (pattern), connection hindgut / posterior midgut, foregut, hindgut, larval brain hemisphere (optic lobes ?), midgut, PNS, segmental pattern (PNS ?)" *F "P{PZ}14-3-3[07103]","CNS (pattern), dorsal vessel, hindgut, midgut, pharynx, segmental pattern, somatic muscles (pattern), visceral mesoderm" *F "P{PZ}l(2)03105[03105]","CNS (pattern), dorsal vessel, larval brain hemisphere, PNS, segmental pattern (epidermis)" *F "P{PZ}l(2)06850[06850]","CNS (pattern), epidermis (segmental pattern), head, larval brain hemisphere (pattern), pharynx, PNS?, posterior spiracles, segmental pattern, staining anterior to cephalic furrow, visceral mesoderm" *F "P{PZ}l(2)09639[09639]","CNS (pattern), epidermis (segmental pattern), larval brain hemisphere (pattern), pharynx, PNS" *F "P{PZ}l(2)06373[06373]","CNS (pattern), epidermis, foregut (weak), hindgut (2 rows), pharynx, PNS (?), proventriculus, segmental pattern (stripes), stomodeal plate" *F "P{PZ}l(2)07054[07054]","CNS (pattern), epidermis, foregut, hindgut, larval brain hemisphere, mesoderm, midgut, PNS, segmental pattern (PNS ?), stomodeum, trachea" *F "P{PZ}l(2)03235[03235]","CNS (pattern), epidermis, hindgut, larval brain hemisphere, PNS, segmental pattern" *F "P{PZ}l(2)06320[06320]","CNS (pattern), foregut, gastric caeca, head, hindgut (weak), lethal phenotype, midgut, PNS, segmental pattern, somatic muscles (pattern), stomodeal plate, trachea (?)" *F "P{PZ}l(2)01810[01810]","CNS (pattern), foregut, head, hindgut, larval brain hemisphere, mesoderm, midgut, oesophagus, PNS, segmental pattern, visceral mesoderm" *F "P{PZ}l(2)03531[03531]","CNS (pattern), gastric caeca, midgut, segmental pattern (neuroblasts ?)" *F "P{PZ}l(2)04111[04111]","CNS (pattern), gonadal mesoderm, larval brain hemisphere, visceral mesoderm" *F "P{PZ}l(2)10521[10521]","CNS (pattern), hindgut (weak), larval brain hemisphere, mesoderm, midgut, PNS, segmental pattern (PNS ?), segmental pattern (stripes), stomodeum" *F "P{PZ}l(2)01467[01467]","CNS (pattern), larval brain hemisphere (pattern), midgut, PNS (pattern), segmental pattern" *F "P{PZ}l(2)01738[01738]","CNS (pattern), larval brain hemisphere (pattern), midgut, pharynx, posterior midgut, segmental pattern" *F "P{PZ}l(2)04884[04884]","CNS (pattern), larval brain hemisphere, Malpighian tubules (?), pharynx (?), PNS, segmental pattern, somatic muscles (?), trachea (?)" *F "P{PZ}fam[05475]","CNS (pattern), larval brain hemisphere, larval brain hemisphere (pattern), pharynx (weak), PNS, posterior midgut, somatic muscles (weak)" *F "P{PZ}l(2)07206[07206]","CNS (pattern), larval brain hemisphere, mesectoderm (?), PNS, segmental pattern (PNS ?), segmental pattern (weak)" *F "P{PZ}l(2)07838[07838]","CNS (pattern), larval brain hemisphere, midgut, PNS, segmental pattern (PNS ?)" *F "P{PZ}l(2)07129[07129]","CNS (pattern), midgut, PNS (?), segmental pattern (PNS ?), visceral mesoderm" *F "P{PZ}dap[04454]","CNS (pattern), oenocytes, segmental pattern (PNS?), visceral mesoderm" *F "P{PZ}l(2)00248[00248]","CNS (pattern), segmental pattern" *F "P{PZ}l(2)05351[05351]","CNS (pattern: midline cells ?), mesoderm, posterior spiracles, segmental pattern, visceral mesoderm" *F "P{PZ}l(2)00231[00231]","CNS (single cells), germ cells, larval brain hemisphere (single cells)" *F "P{PZ}l(2)04065[04065]","CNS, PNS, epidermis (segmental pattern)" *F "P{PZ}l(2)03605[03605]","CNS, dorsal vessel (?), larval brain hemisphere, Malpighian tubules, mesoderm, PNS, proctodeum, stomodeum" *F "P{PZ}Dcp-1[01862]","CNS, ectoderm, hindgut, larval brain hemisphere, mesoderm, midgut (weak), stomodeum" *F "P{PZ}l(2)03728[03728]","CNS, ectoderm, mesoderm, PNS, segmental pattern (epidermis ?), stomodeum" *F "P{PZ}lwr[05486]","CNS, epidermis, larval brain hemisphere" *F "P{PZ}l(2)02074[02074]","CNS, epidermis, larval brain hemisphere, PNS (?), segmental pattern, somatic muscles (?)" *F "P{PZ}l(2)04329[04329]","CNS, larval brain hemisphere, PNS (?), segmental pattern, somatic muscles (?)" *F "P{PZ}l(2)01848[01848]","CNS, larval brain hemisphere, PNS, proctodeum (?), segmental pattern" *F "P{PZ}l(2)10424[10424]","CNS, larval brain hemisphere, larval brain hemisphere (pattern), hypopharyngeal lobe (?), PNS, segmental pattern (PNS), staining anterior to cephalic furrow" *F "P{PZ}l(2)05056[05056]","PNS (?)" *F "P{PZ}chn[02064]","PNS (?), pharynx (?)" *F "P{PZ}l(2)01482[01482]","all cells except pole cells (stage 05), from stage 08: amnioserosa, anterior midgut, CNS, ectoderm, epidermis (segmental pattern), larval brain hemisphere, hindgut, mesoderm, midgut, PNS, posterior midgut" *F "P{PZ}kis[07812]","all nuclei except pole cells (stage 05), amnioserosa, cephalic furrow, CNS (pattern), ectoderm, epidermis (pattern), foregut, hindgut, larval brain hemisphere, mesoderm, midgut, PNS, posterior midgut, proventriculus, segmental pattern, stomodeum" *F "P{PZ}l(2)02029[02029]","all tissues stained" *F "P{PZ}Kr[00895]","amnioserosa, CNS (pattern), dorsal vessel, larval brain hemisphere (pattern), lethal phenotype, PNS, segmental pattern (epidermis?)" *F "P{PZ}drk[10626]","amnioserosa, CNS (pattern), epidermis, foregut, hindgut, larval brain hemisphere, Malpighian tubules ?, mesoderm, midgut, PNS (pattern)" *F "P{PZ}l(2)00297[00297]","amnioserosa, anal plate (?), clypeolabrum, CNS (pattern), epidermis (segmental pattern), foregut, hindgut, mesoderm (?), PNS (pattern), trachea" *F "P{PZ}l(2)05642[05642]","amnioserosa, anterior midgut, CNS (pattern), mesoderm, PNS (pattern), segmental pattern, segmental pattern" *F "P{PZ}tdf[03041]","amnioserosa, midgut, CNS, dorsal vessel, foregut, gonadal mesoderm, head, hindgut, brain hemisphere, Malpighian tubules, midgut, PNS, post. midgut, proctodeal invagination, proctodeum, salivary glands, somatic muscles, stomodeum, trachea, ureter, seg. pat" *F "P{PZ}l(2)03497[03497]","anal plate (?), anterior midgut, CNS (pattern), dorsal ridge, dorsal vessel, hindgut, larval brain hemisphere, Malpighian tubules (?), midgut (weak), pharynx, PNS, posterior spiracle (?), row of cells: dorsal ridge" *F "P{PZ}18w[00053]","anal plate (?), cell groups in procephalic lobe, clypeolabrum, CNS (pattern), hindgut, Malpighian tubules, PNS, proctodeum, salivary gland, segmental pattern, stomodeum" *F "P{PZ}wb[09437]","anal plate (ring), dorsal vessel, epidermis (pattern), pharynx, PNS (?), visceral muscles" *F "P{PZ}l(2)03050[03050]","anal plate, CNS (pattern), dorsal vessel, larval brain hemisphere, midgut, pharynx, PNS, posterior spiracles, proventriculus (imaginal ring ?)" *F "P{PZ}l(2)02535[02535]","anal plate, CNS, hindgut, hindgut imaginal ring, foregut imaginal ring, larval brain hemisphere, oesophagus, pharynx, PNS (?), posterior stripe in maxillary segment, proventriculus (imaginal ring), salivary gland duct (?), visceral mesoderm of hindgut" *F "P{PZ}Pka-C1[01272]","anal plate, anterior spiracles, gastric caeca, hindgut, larval brain hemisphere (pattern), midgut, PNS (?), posterior spiracles, somatic muscles (pattern)" *F "P{PZ}al[05142]","anal region, larval brain hemisphere (optic lobes ?), mandibular segment, midgut (single cells), pharynx, pharynx (median tooth ?), PNS (?), posterior spiracles (?), segmental pattern, single cells (PNS ?), telson (?)" *F "P{PZ}shg[10469]","ant. midgut, chordotonal organ, CNS (patt., midline ?), dorsal vessel, ectoderm, epidermis (patt.), foregut, gastric caeca, hindgut, larval brain hemisphere, Malpighian tubules, mesectoderm ?, mesoderm ?, midgut, PNS, post. midgut, salivary gland, trachea" *F "P{PZ}l(2)04012[04012]","anterior midgut (?), anterior spiracles (?), gastric caeca, hindgut, larval brain hemisphere (weak), mesoderm (weak), midgut (weak), pharynx, PNS, segmental pattern, somatic muscles, trachea" *F "P{PZ}l(2)06270[06270]","anterior midgut, CNS (pattern), dorsal vessel, larval brain hemisphere, Malpighian tubules, PNS" *F "P{PZ}l(2)05643[05643],","anterior midgut, CNS (pattern), epidermis (segmental pattern), foregut, hindgut, larval anus, larval brain hemisphere, Malpighian tubules (?), mesoderm, midgut, PNS (seg. patt.), posterior midgut, seg. patt., anterior cephalic furrow, visceral mesoderm ?" *F "P{PZ}l(2)04154[04154]","anterior midgut, CNS (pattern), epidermis (segmental pattern), larval brain hemisphere, mesoderm" *F "P{PZ}dock[04723]","anterior midgut, CNS (pattern), epidermis (segmental pattern), larval brain hemisphere, oenocytes, pharynx, PNS, segmental pattern" *F "P{PZ}l(2)05070[05070]","anterior midgut, CNS (pattern), epidermis (segmental pattern), mesoderm, PNS (pattern)" *F "P{PZ}l(2)05836[05836]","anterior midgut, CNS (pattern), epidermis, foregut, hindgut, larval brain hemisphere, mesoderm, midgut, PNS (pattern), posterior midgut, stomodeum, trachea" *F "P{PZ}l(2)43Bc[05518]","anterior midgut, CNS (pattern), epidermis, larval brain hemisphere, imaginal disc precursors, midgut, pharynx, PNS, posterior midgut, segmental pattern, stripe anterior to cephalic furrow" *F "P{PZ}l(2)02637[02637]","anterior midgut, CNS (pattern), foregut, hindgut, lethal phenotype, Malpighian tubules, PNS (pattern), posterior midgut, segmental pattern, stomodeum" *F "P{PZ}CycE[05206]","anterior midgut, CNS (pattern), foregut, larval brain hemisphere, hindgut, mesoderm, posterior midgut, segmental pattern (PNS ?)" *F "P{PZ}l(2)06496[06496]","anterior midgut, CNS (pattern), gastric caeca, larval brain hemisphere, lethal phenotype, hindgut, mesoderm, pharynx, PNS, posterior midgut, segmental pattern" *F "P{PZ}l(2)00329[00329]","anterior midgut, CNS (pattern), hindgut, larval brain hemisphere, lethal phenotype, midgut constrictions, PNS (?), posterior midgut, proventriculus, segmental pattern (PNS ?)" *F "P{PZ}l(2)01103[01103]","anterior midgut, CNS (pattern), larval brain hemisphere, hindgut, mesoderm, PNS, segmental pattern" *F "P{PZ}l(2)04493[04493]","anterior midgut, CNS (pattern), larval brain hemisphere, mesoderm, PNS, segmental pattern" *F "P{PZ}l(2)08014[08014]","anterior midgut, CNS (weak), larval brain hemisphere (weak), posterior midgut" *F "P{PZ}Vha68-2[01510]","anterior midgut, CNS / PNS (?), hindgut, posterior midgut" *F "P{PZ}l(2)08269[08269]","anterior midgut, CNS, dorsal vessel, epidermis (segmental pattern), foregut, hindgut, larval brain hemisphere, Malpighian tubules, mesoderm, midgut, PNS, posterior midgut, segmental pattern, somatic muscles (?), trachea" *F "P{PZ}l(2)08717[08717]","anterior midgut, CNS, epidermis (segmental pattern), foregut, gastric caeca, hindgut, larval brain hemisphere, Malpighian tubules, mesoderm, midgut, PNS, posterior midgut, proventriculus, segmental pattern, somatic muscles, trachea (?)" *F "P{PZ}tsh[04319]","anterior midgut, CNS, epidermis, midgut, pharynx" *F "P{PZ}l(2)07768[07768]","anterior midgut, CNS, larval brain hemisphere, mesoderm (?), midgut (single cells), PNS (?), posterior midgut, segmental pattern (PNS), somatic muscles (weak)" *F "P{PZ}l(2)06214[06214]","anterior midgut, PNS (?), segmental pattern" *F "P{PZ}RhoGEF2[04291]","anterior midgut, anterior stripe, CNS (pattern), epidermis (segmental pattern), larval brain hemisphere, mesoderm, oesophagus, PNS, segmental pattern, segmental pattern, visceral mesoderm" *F "P{PZ}l(2)02448[02448]","anterior midgut, clypeolabrum, CNS, ectoderm, hindgut, larval brain hemisphere, Malpighian tubules, mesoderm, PNS, posterior midgut, proctodeum, stomodeum" *F "P{PZ}l(2)08770[08770]","anterior midgut, dorsal vessel, gastric caeca, midgut, pharynx, PNS, proventriculus" *F "P{PZ}l(2)03709[03709]","anterior midgut, epidermis (segmental pattern), foregut, hindgut, Malpighian tubules, mesoderm, posterior midgut" *F "P{PZ}l(2)04820[04820]","anterior midgut, epidermis (segmental pattern), foregut, hindgut, posterior midgut" *F "P{PZ}l(2)05428[05428]","anterior midgut, epidermis (segmental pattern), gastric caeca" *F "P{PZ}tkv[04415]","anterior midgut, epidermis (segmental pattern), hindgut, Malpighian tubules, posterior midgut, segmental pattern, trachea" *F "P{PZ}vkg[01209]","anterior midgut, fat body, gastric caeca, mesoderm, midgut, pericardial cells, posterior midgut, proventriculus, single cells (hemocytes ?)" *F "P{PZ}l(2)04418[04418]","anterior ring of cells, CNS, larval brain hemisphere, mandible (?), PNS (segmental pattern), segmental pattern" *F "P{PZ}l(2)01351[01351]","anterior spiracles (?), CNS (pattern), Malpighian tubules, midgut, pharynx, PNS (?), posterior spiracles, trachea (pattern)" *F "P{PZ}l(2)01501[01501]","anterior spiracles (?), CNS (pattern), larval brain hemisphere (pattern), lethal phenotype (?), midgut, PNS (?)" *F "P{PZ}dpp[10638]","anterior spiracles (?), CNS (pattern), larval brain hemisphere, PNS, segmental pattern (PNS ?)" *F "P{PZ}l(2)06736[06736]","anterior spiracles, clypeolabrum, CNS (pattern), larval brain hemisphere (pattern), midgut, pharynx, posterior spiracles, trachea (pattern)" *F "P{PZ}l(2)06955[06955]","apodemes, CNS (pattern), hindgut, larval brain hemisphere, mesoderm, midgut (weak), PNS (?), segmental pattern, somatic muscles, stomodeum, visceral mesoderm" *F "P{PZ}l(2)10481[10481]","cephalic furrow, clypeolabrum, CNS (pattern), larval brain hemisphere (pattern), PNS, segmental pattern" *F "P{PZ}S[07056]","clypeolabrum, CNS (pattern), foregut, head, hindgut, larval brain hemisphere (pattern), mesoderm (weak), pharynx, PNS, posterior midgut, posterior spiracles, proventriculus, segmental pattern (PNS), ventral midline, ventral surface" *F "P{PZ}l(2)05337[05337]","clypeolabrum, CNS, hindgut, Malpighian tubules, PNS, posterior spiracles (?), segmental pattern, stomodeum" *F "P{PZ}l(2)01857[01857]","clypeolabrum, pattern in maxillary segment (?), ring gland (?)" *F "P{PZ}l(2)00064[00064]","dorsal vessel, epidermis (segmental pattern), larval brain hemisphere, Malpighian tubules, midgut (?), pharynx, PNS (?)" *F "P{PZ}l(2)07022[07022]","hemisphere, pattern in larval brain hemisphere, PNS (?), posterior midgut (single cells), trachea (single cells)" *F "P{PZ}l(2)02107[02107]","hindgut (anterior), larval brain hemisphere, PNS (?)" *F "P{PZ}l(2)04227[04227]","hindgut, pharynx, PNS (?), proventriculus, somatic muscles" *F "P{PZ}l(2)04431[04431]","larval brain hemisphere (optic lobes), one single cell per hemisegment, PNS" *F "P{PZ}l(2)10642[10642]","larval brain hemisphere (pattern), pattern on CNS, pharynx (weak), PNS, posterior spiracles" *F "P{PZ}l(2)06444[06444]","larval brain hemisphere, lethal phenotype, PNS" *F "P{PZ}l(2)01361[01361]","lethal phenotype, no embryonic pattern (background)" *F "P{PZ}l(2)02047[02047]","lethal phenotype, no embryonic pattern (background)" *F "P{PZ}l(2)05488[05488]","lethal phenotype, no embryonic pattern (background)" *F "P{PZ}l(2)01038[01038]","no embryonic pattern (background ?)" *F "P{PZ}l(2)01085[01085]","no embryonic pattern (background ?)" *F "P{PZ}l(2)01288[01288]","no embryonic pattern (background ?)" *F "P{PZ}l(2)03068[03068]","no embryonic pattern (background ?)" *F "P{PZ}l(2)03845[03845]","no embryonic pattern (background ?)" *F "P{PZ}l(2)05006[05006]","no embryonic pattern (background ?)" *F "P{PZ}l(2)05287[05287]","no embryonic pattern (background ?)" *F "P{PZ}l(2)05327[05327]","no embryonic pattern (background ?)" *F "P{PZ}l(2)05714[05714]","no embryonic pattern (background ?)" *F "P{PZ}l(2)08492[08492]","no embryonic pattern (background ?)" *F "P{PZ}Dcp-1[02132]","no embryonic pattern (background)" *F "P{PZ}Eif4a[02439]","no embryonic pattern (background)" *F "P{PZ}mts[02496]","no embryonic pattern (background)" *F "P{PZ}l(2)01265[01265]","no embryonic pattern, cell death" *F "P{PZ}l(2)08774[08774]","pharynx, PNS, somatic muscles (background)" *F "P{PZ}l(2)02306[02306]","segmental pattern (PNS ?), anterior midgut, posterior midgut (weak)" *F "P{PZ}l(2)04530[04530]","weak staining" *F "P{PZ}l(2)10523[10523]","weak staining in all stages" *F "P{PZ}l(2)10685[10685]","weak staining in all stages, background (?)" # *U FBrf0103417 *a Pinter *b M. *t 1998.7.1 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Jun 26 12:27:03 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 26 Jun 1998 12:27:03 +0100 *F To: marianna@u.arizona.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 26 Jun 1998 12:37:02 +0100 *F Content-Length: 2149 *F Dear Dr. Pinter, *F I am curating your paper for FlyBase: *F Pinter et al., 1998, Insect Biochem. Molec. Biol. 28(2): 91--98 *F and I have some questions about the relationships between the various *F Drosophila calpain genes. *F TER94 corresponds to the Ca2+-activated neutral protease (CANP) activity *F purified and studied in Pinter et al, 1992, Biochemistry 31: 8201--8206. *F I would like to know what the relationship is between this CANP activity *F and the Ca2+-dependent proteolytic activities studied in Pinter and *F Friedrich, 1988, Biochem. J. 253: 467--473. *F In Pinter and Friedrich, 1988, Biochem. J. 253: 467--473, 2 *F Ca2+-dependent-proteinase activities are purified. These are a calpain *F II-like activity (rightmost peak in Figure 2.) and a calpain I-like *F activity (Figure 3). *F Is the calpain-II like activity in this paper the same as the CANP activity *F in Pinter et al, 1992, Biochemistry 31: 8201--8206 (i.e is it TER94) ? *F Do you know whether the calpain-I like activity in Pinter and Friedrich, *F 1988, Biochem. J. 253: 467--473 corresponds to any of the other known *F Drosophila calpain genes, which I list below, or is it a different calpain *F gene ? *F a) CalpA gene, at 56C-56D (this was cloned in J. Biol. Chem. *F 269: 25137--25142, where it is referred to as Dm-calpain). *F b) Calp14B, at 14C. This comes from a PhD thesis: *F Rutherford, 1995, Ph.D. Thesis, University of California at San Diego, CA *F 'The genetic and biochemical characterization of the major cyclophilin *F isoform in Drosophila melanogaster.' *F where a region of homology to a vertebrate calpain was found proximal to *F the Cyp-1 gene. *F c) sol - small optic lobes *F this gene also has homology to vertebrate calpains, and maps to 19F. *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From marianna@u.arizona.edu Sat Jun 27 04:16:44 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 27 Jun 1998 04:16:44 +0100 *F Date: Fri, 26 Jun 1998 20:27:01 -0700 (MST) *F From: Marianna Pinter *F Reply-To: Marianna Pinter *F To: Gillian Millburn *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F On Fri, 26 Jun 1998, Gillian Millburn wrote: *F Dear Gillian, *F I attempt to answer your questions. It might well be You won't *F find my answers satisfying, - You won't get simple yes or no. Though I try *F my best. And You are always wellcome to continue asking. *F Best regards, *F Marianna *F > Dear Dr. Pinter, *F > *F > I am curating your paper for FlyBase: *F > *F > Pinter et al., 1998, Insect Biochem. Molec. Biol. 28(2): 91--98 *F > *F > and I have some questions about the relationships between the various *F > Drosophila calpain genes. *F > *F > TER94 corresponds to the Ca2+-activated neutral protease (CANP) *F activity *F UNFORTUNATELY I CAN NOT SAY SO. I HAVE CLONED TER94 BY THE AIDS OF AN *F ANTI-CANP ANTIBODY. TER94 ITSELF SHOWS NO SIMILARITY TO CALPAINS OR ANY *F OTHER PROTEASES. IN SUCH A CASE ONE SHOULD PROVE THAT TER94 IS REALLY A *F PROTEASE. I HAVE NOT SUCCEEDED: THE EXPRESSED GST-TER94 DID NOT SHOW *F CA2+-ACTIVATED PROTEASE ACTIVITY. *F > TER94 corresponds to the Ca[2+]-activated neutral protease (CANP) activity *F > purified and studied in Pinter et al, 1992, Biochemistry 31: 8201--8206. *F > I would like to know what the relationship is between this CANP activity *F > and the Ca[2+]-dependent proteolytic activities studied in Pinter and *F > Friedrich, 1988, Biochem. J. 253: 467--473. *F CANP (BIOCHEMISTRY) HAS BEEN PURIFIED. THE TWO CA2+-ACTIVATED PROTEASE *F (BIOCHEM J.) HAS BEEN DETECTED RATHER, OR IF YOU LIKE: PARTIALLY PURIFIED. *F THE RESINS USED FOR LIQUID CHROMATOGRAPHY ARE DIFFERENT: IT DOES NOT HELP *F TO IDENTIFY CANP (BIOCHEMISTRY) AS ANY OF THE PREVIOUSLY DETECTED *F CA2+-ACTIVATED PROTEASE (BIOCHEM.J.). IN BOTH CASES (BIOCHEM. & *F BIOCHEM.J.) I HAVE USED CYTOSOL FRACTION TO START WITH AND THE *F CA2+-DEPENDENT PROTEASE (BIOCHEM.J.) WITH HIGHER CA2+ CONCENTRATION *F REQUIREMENT AND CANP (BIOCHEM.J.) SHOWS SIMILAR CA2+-SENSITIVITY. THESE *F TWO MAY BE THE SAME. *F ONE CAN SAY THAT THERE ARE TWO OR THREE CYTOSOLIC CA2+-DEPENDENT *F PROTEASES, WHICH HAVE BEEN IDENTIFIED IN DROSOPHILA: ONE OF THESE NEEDS *F LOW CA2+ CONCENTRATION TO BE ACTIVATED AND THE OTHER TWO (WHICH MAY BE THE *F SAME) NEED HIGH. *F > *F > In Pinter and Friedrich, 1988, Biochem. J. 253: 467--473, 2 *F > Ca[2+]-dependent-proteinase activities are purified. These are a calpain *F > II-like activity (rightmost peak in Figure 2.) and a calpain I-like *F > activity (Figure 3). *F > *F > Is the calpain-II like activity in this paper the same as the CANP activity *F > in Pinter et al, 1992, Biochemistry 31: 8201--8206 (i.e is it TER94) ? *F SEE MY PREVIOUS NOTE. *F > *F > Do you know whether the calpain-I like activity in Pinter and Friedrich, *F > 1988, Biochem. J. 253: 467--473 corresponds to any of the other known *F > Drosophila calpain genes, which I list below, or is it a different calpain *F > gene ? *F > *F > a) CalpA gene, at 56C-56D (this was cloned in J. Biol. Chem. *F > 269: 25137--25142, where it is referred to as Dm-calpain). *F PROBABLY NOT, SINCE CALPA IS NOT DETECTED IN THE CYTOSOLE WITH THE *F ANTIBODY WHICH I HAVE USED TO CLONE IT. *F > *F > b) Calp14B, at 14C. This comes from a PhD thesis: *F > *F > Rutherford, 1995, Ph.D. Thesis, University of California at San Diego, CA *F > *F > 'The genetic and biochemical characterization of the major cyclophilin *F > isoform in Drosophila melanogaster.' *F > *F > where a region of homology to a vertebrate calpain was found proximal to *F > the Cyp-1 gene. *F I HAVE NEVER HEARD ABOUT THIS THESIS, THOUGH I WOULD BE VERY INTERESTED TO *F READ. TO TELL THE TRUTH, I HAVE BEEN LOOKING FOR NEW PUTATIVE CALPAIN *F GENES IN THE DATABASE RECENTLY AND I COULD NOT FIND ANY. *F > *F > *F > c) sol - small optic lobes *F > *F > this gene also has homology to vertebrate calpains, and maps to 19F. *F > *F I HAVE CHECKED SOL STRAIN FOR CA2+-ACTIVATED PROTEASE ACTIVITY AND IT IS *F INDISTINGUISABLE FORM WILD TYPE STRAIN (CANTON S). THE EXPERIMENT HAS BEEN *F PERFORMED AS DESCRIBED IN THE BIOCEM.J. ARTICLE: CYTOSOLE FRACTION, PH4.5 *F FRACTIONATION, THEN ACTIVITY MEASUREMENT. *F THE SOL PROTEIN SHOULD BE A PROTEASE. THOUGH NEITHER SOL OR OTHER GENES *F WHICH SHOW HIGH SIMILARITY TO THE PROTEASE DOMAIN OF CALPAINS NOT *F NECESSARILY ENCODES CA2+-DEPENDENT PROTEASES. CA2+-DEPENDENT PROTEASE *F ACTIVITY HAS NOT YET PROVED IN THE CASE OF ANY OF THESE INTERESTING *F PROTEINS. PERSONALLY I DON'T SEE ANY REASON TO CALL THEM CALPAIN: CALPAINS *F ARE BY DEFINITION CA2+-ACTIVATED CYSTEINE PROTEASES. *F > *F > I look forward to hearing from you, *F > *F > Gillian Millburn *F > *F > -------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > -------------------------------------------------------------- *F > *F \--------------------- *F Marianna Pinter *F marianna@U.Arizona.EDU *F Department of Molecular &Cellular Biology *F The University of Arizona *F LSS Bldg, 1007 E. Lowell Street, PO bOx 210106 *F TUCSON AZ 85723-0106, USA *F From gm119@gen.cam.ac.uk Tue Jun 30 10:31:13 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 Jun 1998 10:31:13 +0100 *F To: marianna@U.Arizona.EDU *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 30 Jun 1998 10:41:35 +0100 *F Content-Length: 3496 *F Dear Marianna, *F thankyou for your reply, it was very helpful. I would like to curate the *F information in your e-mail as a personal communication from you to *F FlyBase. *F Here is what I propose: *F 1. We currently have the Ca[2+]-activated neutral protease (CANP) activity *F purified and studied in Pinter et al, 1992, Biochemistry 31: 8201--8206 as *F Ca-TPase. *F I will create 2 new genes for the Ca[2+]-dependent-proteinase activities *F studied in Pinter and Friedrich, 1988, Biochem. J. 253: 467--473. Is it OK *F if I call them CalpI and CalpII (for Calpain-I and Calpain-II) ? or would *F you rather I call them Ca-PaseI and Ca-PaseII (for Ca[2+]-dependent *F proteinase I and II) ? *F I will put a comment saying that the relationship between each of these *F genes and Ca-TPase is unknown. *F 2. I will put a comment saying that it is likely that Ca-TPase, and the 2 *F proteinases from Pinter and Friedrich, 1988, Biochem. J. 253: 467--473 are *F not the same as CalpA, as they are purified from the cytosol and CalpA is *F not detected in the cytosol with an anti-CalpA antibody. *F 3. I will also put a comment saying that @sol@[-] flies have *F Ca[2+]-activated protease activity that is indistinguishable from *F wild-type. *F I would be grateful if you could tell me whether the proposals I have made *F for naming the various Ca-dependent proteinases above are OK. Thankyou *F once again for helping to sort this out, *F Gillian *F From marianna@u.arizona.edu Tue Jun 30 22:31:29 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 Jun 1998 22:31:29 +0100 *F Date: Tue, 30 Jun 1998 14:41:29 -0700 (MST) *F From: Marianna Pinter *F X-Sender: marianna@orion *F Reply-To: Marianna Pinter *F To: Gillian Millburn *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Dear Gillian, *F I hope my answers on your points will help You in the work. *F Regards, *F Marianna *F On Tue, 30 Jun 1998, Gillian Millburn wrote: *F > Dear Marianna, *F > *F > thankyou for your reply, it was very helpful. I would like to curate the *F > information in your e-mail as a personal communication from you to *F > FlyBase. *F > *F > Here is what I propose: *F > *F > *F > 1. We currently have the Ca[2+]-activated neutral protease (CANP) activity *F > purified and studied in Pinter et al, 1992, Biochemistry 31: 8201--8206 as *F > Ca-TPase. *F I would suggest to use the name CANP. It stands fo Ca2+-activated neutral *F protease. To introduce a new name usually makes confusion. Here I don't *F see any point to do that. (In addition, Ca-TPase rhymes with ATPase and I *F don't find it especially good choice.) *F > *F > I will create 2 new genes for the Ca[2+]-dependent-proteinase activities *F > studied in Pinter and Friedrich, 1988, Biochem. J. 253: 467--473. Is it OK *F > if I call them CalpI and CalpII (for Calpain-I and Calpain-II) ? or would *F > you rather I call them Ca-PaseI and Ca-PaseII (for Ca[2+]-dependent *F > proteinase I and II) ? *F I think CalpI and CalpII would be a safe and traditional solution. *F > *F > I will put a comment saying that the relationship between each of these *F > genes and Ca-TPase is unknown. *F You might say a little more. CalpI is not the same as CalpII or CANP, - *F that's for sure. And the relation between CalpII and CANP is unknown. *F > *F > 2. I will put a comment saying that it is likely that Ca-TPase, and the 2 *F > proteinases from Pinter and Friedrich, 1988, Biochem. J. 253: 467--473 are *F > not the same as CalpA, as they are purified from the cytosol and CalpA is *F > not detected in the cytosol with an anti-CalpA antibody. *F That's fine, - but use CANP please instead os Ca-TPase. *F > *F > 3. I will also put a comment saying that @sol@[-] flies have *F > Ca[2+]-activated protease activity that is indistinguishable from *F > wild-type. *F That's OK. *F \--------------------- *F Marianna Pinter *F marianna@U.Arizona.EDU *F Department of Molecular &Cellular Biology *F The University of Arizona *F LSS Bldg, 1007 E. Lowell Street, PO bOx 210106 *F TUCSON AZ 85723-0106, USA *F From gm119@gen.cam.ac.uk Wed Jul 01 09:01:52 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 1 Jul 1998 09:01:52 +0100 *F To: marianna@U.Arizona.EDU *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 1 Jul 1998 09:12:10 +0100 *F Content-Length: 3626 *F Dear Marianna, *F > > 1. We currently have the Ca[2+]-activated neutral protease (CANP) activity *F > > purified and studied in Pinter et al, 1992, Biochemistry 31: 8201--8206 as *F > > Ca-TPase. *F > > *F > I would suggest to use the name CANP. It stands fo Ca2+-activated neutral *F > protease. To introduce a new name usually makes confusion. Here I don't *F > see any point to do that. (In addition, Ca-TPase rhymes with ATPase and I *F > don't find it especially good choice.) *F I will use the name CANP, for Ca2+-activated neutral protease. *F > > *F > > I will create 2 new genes for the Ca[2+]-dependent-proteinase activities *F > > studied in Pinter and Friedrich, 1988, Biochem. J. 253: 467--473. Is it OK *F > > if I call them CalpI and CalpII (for Calpain-I and Calpain-II) ? or would *F > > you rather I call them Ca-PaseI and Ca-PaseII (for Ca[2+]-dependent *F > > proteinase I and II) ? *F > *F > I think CalpI and CalpII would be a safe and traditional solution. *F > *F OK *F > > I will put a comment saying that the relationship between each of these *F > > genes and Ca-TPase is unknown. *F > *F > You might say a little more. CalpI is not the same as CalpII or CANP, - *F > that's for sure. And the relation between CalpII and CANP is unknown. *F I will add the extra information you suggest. *F > *F > > *F > > 2. I will put a comment saying that it is likely that Ca-TPase, and the 2 *F > > proteinases from Pinter and Friedrich, 1988, Biochem. J. 253: 467--473 are *F > > not the same as CalpA, as they are purified from the cytosol and CalpA is *F > > not detected in the cytosol with an anti-CalpA antibody. *F > *F > That's fine, - but use CANP please instead os Ca-TPase. *F I will use CANP. *F Is all that OK ? *F Gillian *F From marianna@u.arizona.edu Wed Jul 01 19:53:21 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 1 Jul 1998 19:53:21 +0100 *F Date: Wed, 1 Jul 1998 12:03:37 -0700 (MST) *F From: Marianna Pinter *F X-Sender: marianna@orion *F To: Gillian Millburn *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F That's all fine, Gillian. *F Regards, *F Marianna *F \--------------------- *F Marianna Pinter *F marianna@U.Arizona.EDU *F Department of Molecular &Cellular Biology *F The University of Arizona *F LSS Bldg, 1007 E. Lowell Street, PO bOx 210106 *F TUCSON AZ 85723-0106, USA # *U FBrf0103479 *a Van Daele *b E. *t 1998.8.11 *T personal communication to FlyBase *u *F From matthewk@fly.bio.indiana.edu Wed Aug 12 01:38:10 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Aug 1998 01:38:10 +0100 *F Date: Tue, 11 Aug 98 19:32:52 EST *F From: 'Kathy Matthews' *F To: flybase-updates@morgan.harvard.edu *F Subject: pc *F Content-Length: 359 *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Eddy Van Daele *F To: Bloomington Drosophila Stock Center *F Dated: 7 July 1998 and 11 Aug 1998 *F Information communicated: *F Df(2R)knSA3 complements scb01288 and boc1 *F Df(2R)knSA3 fails to complement l(2)k00103k00103 *F Df(2R)knSA3 fails to complement Df(2R)Jp1 and Df(2R)XTE-18 # *U FBrf0103497 *a Griffin-Shea *b R. *t 1998.8.12 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Ruth Griffin-Shea, CEA-Grenoble *F To: Bloomington Drosophila Stock Center *F Subject: Df(3R)rn20 *F Dated: 12 August 1998 *F *F Background: The current FlyBase record for Df(3R)rn20 indicates that one breakpoint may be within the rn gene, creating the allele rn[20]. *F *F Information communicated: *F Df(3R)rn20 removes the whole rotund region and nearby genes. # *U FBrf0103498 *a Carpenter *b A.T.C. *t 1998.8.17 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Thu Aug 13 17:21:18 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Aug 1998 17:21:18 +0100 *F To: gm119@gen.cam.ac.uk *F Subject: pc to curate *F Cc: ag24@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Aubrey de Grey *F Date: Thu, 13 Aug 1998 17:21:01 +0100 *F Content-Length: 144 *F hi *F From: Adelaide T C Carpenter *F Info communicated: l(2)1052310523 is an allele of bur. # *U FBrf0103510 *a Zhang *b Y. *t 1998.7.13 *T personal communication to FlyBase *u *F From yzhang@biology.utah.edu Mon Jul 13 18:34:16 1998 *F To: rd120@gen.cam.ac.uk *F Subject: correction of cytology of In(1)ombH31 *F Dear Rachel *F I am sending you the extracted communication between Dr Gert Pflugfelder *F and me about the cytology of In(1)ombH31. I think we should get the *F cytology of In(1)ombH31 corrected in Flybase. *F With regards/Yong *F >Dear Dr Gert Pflugfelder *F > *F >To my results, the proximal point of In(1)omb is not 12D2-E1, but 14D2-E1; *F >The distal breakpoint is consistent with previous data, at 4C6. Any *F >comments?? *F >With regards, Yong *F Dear Dr. Zhang, *F thank you for your information on the proximal breakpoint of In(1)ombH31. *F It looks like a typo was introduced into the literature some 20 years ago *F and stayed. *F Best regards Gert Pflugfelder # *U FBrf0103512 *a Cavener *b D. *t 1998.7.17 *T personal communication to FlyBase *u *F >From dcavener@ctrvax.vanderbilt.edu Fri Jul 17 14:16:55 1998 *F To: flybase-help@morgan.harvard.edu *F The eIF-2alpha gene is not on the main map (14C8-D1). *F Minor errors aside, FLYBASE has become a really terrific tool for our research. *F Thanks! *F Doug Cavener *F >From ag24@gen.cam.ac.uk Fri Jul 17 14:35:25 1998 *F To: dcavener@ctrvax.Vanderbilt.Edu *F Subject: Re: sb and Eif *F Hi Doug, *F > Eif2alpha ... (14C8-D1) *F Evidently you know something we don't... how do you know that? *F If this is your own localisation, please send full info (was it in *F situ or Df mapping, etc.) and we will curate it as a pers comm. *F Cheers, Aubrey *F >From dcavener@ctrvax.vanderbilt.edu Fri Jul 17 15:11:23 1998 *F From: dcavener@ctrvax.Vanderbilt.Edu (Douglas R. Cavener) *F Subject: Re: sb and Eif *F Aubrey, *F eIF-2alpha maps to 14C8-D1 based upon the following unpublished data: *F 1. In situ hybridization to salivary gland chromosomes. *F 2. Molecular mapping relative to two P1 clones that in situ map to 14C8-D1 *F 3. Molecular mapping relative to para; eIF-2alpha is within 20kb of *F the 3' end of para, which maps to 14 D1. The gene order is therefore *F eIF-2alpha - para - centromere. *F You can use the above information to update eIF-2alpha and reference as *F personal communication. *F Thanks, Doug *F >From ag24@gen.cam.ac.uk Fri Jul 17 15:25:11 1998 *F To: dcavener@ctrvax.Vanderbilt.Edu *F Subject: Re: sb and Eif *F Thanks. The data will be curated as a pers comm; could you also please *F tell me the names of the two P1 clones, and which way round eIF-2alpha *F is (ie tandem or convergent with para)? *F >From dcavener@ctrvax.vanderbilt.edu Fri Jul 17 15:53:44 1998 *F From: dcavener@ctrvax.Vanderbilt.Edu (Douglas R. Cavener) *F Subject: Re: sb and Eif *F eIF-2alpha corresponds to STS Dm2380, Contig DS06440. This information is *F actually in the E of D (now defunct). (I can't run the new version of the *F molecular maps for some reason.) para and eIF-2alpha transcription units *F are in tandem. *F Doug *F Douglas R. Cavener *F Department of Molecular Biology *F Box 1820 Station B *F Vanderbilt University *F Nashville, TN 37235 *F voice: 615:322-3418 *F fax: 615-343-6707 *F Shipping Address: *F MBIO/Stevenson 2527 *F 1161 21st Avenue South *F Vanderbilt University *F Nashville, TN 37232 # *U FBrf0103515 *a Stern *b M. *t 1998.7.24 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Jul 20 10:55:26 1998 *F To: stern@bioc.rice.edu *F Subject: Helping FlyBase: two RpL27A genes? *F Dear Michael, *F I am writing to you with my FlyBase hat on, for an opinion. *F Its about two papers, one of which is one of yours. *F \*x FBrf0067211 == Garwood and Lepesant, 1994, Biochem. biophys. Res. Commun. 198(2): 748--754 *F \*x FBrf0093217 == Soehnge et al., 1997, Gene 185(2): 257--263 *F Normal literature curation has generated two FlyBase entries for fly genes *F for 'ribosomal protein L27a', which we have termed Rp27Aa (at 87F--88A) *F Rp27Ab (at 24F). We created the two genes because the mapping data *F suggested two distinct genes, far apart in the genome. In the course of *F doing some sorting out and molecular curation we have had cause to check *F this out, and it seems clear from the sequence data that there is actually *F only one gene. *F So, where is it? *F I am writing to Garwood and Lepesant also, but would grateful for any *F input on this that you can give me. *F regards, *F Rachel. *F From stern@bioc.rice.edu Mon Jul 20 17:08:40 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: two RpL27A genes? *F If there is only one L27 gene it is definitely at 24F/25A because we have *F genomic sequence for that gene in a phage clone that also contains some *F inebriated sequence, which is unquestionably at 24F/25A. I can't really *F say whether there is or is not a second gene, although I suspect that there *F isn't. *F Regards, *F Michael. *F From rd120@gen.cam.ac.uk Tue Jul 21 10:58:15 1998 *F To: stern@bioc.rice.edu *F Subject: Re: Helping FlyBase: two RpL27A genes? *F Hi Mike, *F >However if there is only one L27 gene it is *F >definitely at 24F/25A because we have genomic sequence for that gene in a *F >phage clone that also contains some inebriated sequence, which is *F >unquestionably at 24F/25A. *F I detect a snippet of information here! Are you (quietly and shyly) *F telling me that the Gs1l gene is inebriated? *F Rachel. *F From stern@bioc.rice.edu Tue Jul 21 21:01:39 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: two RpL27A genes? *F Hi Rache, no, no, no! Gs1l isn't inebriated, inebriated is a *F neurotransmitter transporter that we published about two years ago. I can *F refer you to PNAS volume 93, pages 13262-13267, which is a reference I got *F out of flybase itself. *F From rd120@gen.cam.ac.uk Wed Jul 22 09:23:09 1998 *F To: stern@bioc.rice.edu *F Subject: Re: Helping FlyBase: two RpL27A genes? *F hiya, *F Your paper said RpL27A was next to Gs1l *F Your mail said (at least some of) RpL27A was in the same phage clone as (at *F least some of) ine *F therefore the order is probably Gs1l-RpL27A-ine *F ? *F bye for now, *F Rachel. *F From stern@bioc.rice.edu Thu Jul 23 19:41:13 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: two RpL27A genes? *F Hi, the order is: telomere-L27-GS1l-ine-centromere. *F Dr. Mikey # *U FBrf0103516 *a Bloomington Drosophila Stock Center *b ?. *t 1998.7.24 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: l(1)TW24 *F Date: 25 July 1998 *F *F Information communicated: *F *F Gene symbol l(1)TW24 *F Allele symbol l(1)TW24[1] *F Map location chromosome 1 *F Balancer variant FM7, l(1)TW24[1] *F Discoverer Ted Wright # *U FBrf0103517 *a Gatti *b M. *t 1998.8.3 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Fri Jul 31 16:59:26 1998 *F To: rd120@gen.cam.ac.uk *F Subject: pc *F mei-68 is a male sterile allele of UbcD1 (Cenci et al Genes Dev., *F 11:863-875, 1997). This mutant exhibits extensive chromosome breakage *F during male meiosis due to the lack of resolution of the telomeric fusions. *F In addition it shows a few telomeric attachments in brain cells. *F Ciao *F Maurizio *F Maurizio Gatti, *F Dipartimento di Genetica e Biologia Molecolare *F Universita' di Roma 'La Sapienza' *F P. A. Moro, 5 00185 Roma, Italy *F Tel: 39-6-49912842; Fax: 39-6-4456866 # *U FBrf0103518 *a Cavener *b D. *t 1998.8.4 *T personal communication to FlyBase *u *F >From dcavener@ctrvax.vanderbilt.edu Tue Aug 04 15:11:57 1998 *F To: Aubrey de Grey *F Aubrey, *F I have an update on the cytological position of bv (brevis). It is *F located within 100A2;100C2-100C3 based upon its failure to complement *F Df(3R)tlle. This is consistent with earlier mapping reported in Flybase. *F Cheers, Doug # *U FBrf0103519 *a McKearin *b D. *t 1998.8.4 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personnal communication from: Dennis McKearin, University of Texas, Southwestern Medical Center *F To: Bloomington Drosophila Stock Center *F Subject: new stonewall alleles *F Dated: 4 August 1998 *F *F Information communicated: *F *F 'My lab has checked two P-lethals at 70D, #P1227 and P2087, for allelism to the female sterile stonewall gene. Both carry P-elements in *F stonewall gene sequences. Both are viable but female sterile over Df(3L)fzM21 which removes stonewall. Both are also viable but sterile *F over previously identified stonewall alleles in our collection. Therefore, these are new stonewall alleles, almost certainly caused by the *F P-element inserts, on chromosomes that are mutant for a separate, essential locus of unknown identity.' *F *F Note from K.M.: *F stock P1227 carries P{lacW}l(3)s9999[s9999] *F stock P2087 carries P{lacW}l(3)j6C3[j6C3] *F *F # *U FBrf0103520 *a Bonini *b N. *t 1998.7.7 *T personal communication to FlyBase *u *F From nbonini@sas.upenn.edu Tue Jul 07 17:07:50 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase *F We have a lacZ near eya, but it is quite far from the gene and I have no *F idea if it is really eya. We have been trying to get evidence that it does *F reflect eya, but do not have it yet. It causes no phenotype. The *F insertion is of the P{lacW} enhancer trap element (as in as in Mozer and *F Benzer, Development 120: 1049-1058 (1994)) and the insertion identifer is *F Il-56B. *F Nancy # *U FBrf0103521 *a Kiss *b I. *t 1998.8.6 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Istvan Kiss, Hungarian Academy of Sciences *F To: Bloomington Drosophila Stock Center *F Dated: 6 August 1998 *F Subject: variegation of y[+] in P{Car20y}EW1 *F *F Information communicated: *F *F The y[+t7.7] allele carried by P{Car20y} is subject to variegation in the P{Car20y}EW1 insertion into CyO (CyO-vWb). *F Expression of the y[+] minigene is good in larvae, allowing straightforward selection of the balancer in an otherwise y[-] *F background. In adults, however, essentially all show some degree of variegation. # *U FBrf0103522 *a Beaton *b A. *t 1998.8.6 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Amy Beaton, Berkeley Drosophila Genome Project *F To: Bloomington Drosophila Stock Center *F Subject: y[+] variegation in CyO-vWb is background dependent *F Dated: 5 August 1998 *F *F Information communicated: *F *F The degree of variegation of y[+t7.7] in CyO-vWb is background dependent. In most BDGP insertion lines carrying this balancer, *F y[+] expression can be detected only in a few macrochaetae and in the presutural, super alar or post alar bristles. Some lines, *F however, have much more extensive y[+] expression in adults. # *U FBrf0103525 *a Hirsh *b J. *t 1998.8.7 *T personal communication to FlyBase *u *F From jh6u@virginia.edu Fri Aug 07 18:54:27 1998 *F To: flybase-help@morgan.harvard.edu *F Subject: P lethal info *F BDGP/Flybase: *F Some additional information on your P lethal l(2)k02104, =Bloomington stock *F P508: *F From the inverse PCR sequence (Genbank accession AQ025704), This P has *F inserted into the last exon of Ddc, & I find that it is non-complementing *F for Ddc. *F Jay Hirsh *F Professor *F Dept of Biology *F University of Virginia *F Charlottesville, VA 22903 *F FAX: 804-982-5626 *F Ph: 804-982-5608 *F jh6u@virginia.edu # *U FBrf0103526 *a Crowley *b T. *t 1998.8.9 *T personal communication to FlyBase *u *F From tc45@columbia.edu Sun Aug 09 19:51:24 1998 *F To: flybase-updates@morgan.harvard.edu *F Subject: Trf-proximal *F Re: FBgn 0013531 *F Recent work in Roger Kornberg's lab at Stanford, described in Jiang et al. *F PNAS 95:8538-8543, shows that the protein coded by the gene which until now *F I have referred to as Trf-proximal, is similar in sequence to a component of *F the mammalian Mediator complex. Mediator is composed of at least 15 *F subunits, is associated with the Carboxy Terminal Domain (CTD) of RNA *F Polymerase II and has been shown to stimulate basal level transcription, *F play a critical role in activated transcription and induce phosphorylation *F of the CTD by the general transcription factor TFIIH. The 41% amino acid *F sequence identity between the fly and mammalian proteins suggests that *F Trf-proximal codes for a component of Mediator in Drosophila, and since its *F expression has only been detected in spermatocytes, it may play a role in *F transcription regulation during spermatogenesis. *F I propose renaming this gene Transcription mediator-related (Tmr) *F and the encoded protein Transcription Mediator-Related (TMR). Please let me *F know if this name is acceptable and update the Flybase entry info as *F indicated above. Also, please add the following citation to this entry and *F to FBgn0010287 (Trf) and FBgn0020240 (Mcr): *F Crowley, T.E. (1998) *F Mutations near the Trf cluster cause a premeiotic defect in the Drosophila *F male germ line. DIS 81, in press. *F Thanks, *F Tom Crowley # *U FBrf0103527 *a Perrimon *b N. *t 1998.8.10 *T personal communication to FlyBase *u *F >From perrimon@rascal.med.harvard.edu Mon Aug 10 09:27:30 1998 *F To: gelbart@morgan.harvard.edu (William Gelbart) *F Subject: Re: Your unstrung gene record *F Bill, *F You can actually delete this. This is a long story but the initial name *F was given based on the revertant phenotype of an enhancer trap that ended *F up deleting more than the initial gene that we were interested in. We *F eventually published, under the name kekkon1 Musacchio, M., Perrimon, N. *F (1996) The Drosophila kekkon genes: novel members of both the leucine-rich *F repeat and immunoglobulin superfamilies expressed in the CNS. Dev Biol 178 *F 63-76., the gene that corresponded to the enhancer trap. *F Best wishes, *F Norbert *F >Norbert, *F > *F >I'm trying to clean up some old, questionable FlyBase gene records. *F > *F >FlyBase has the following gene record, for which the only reference *F >is your 1991 abstract. Is this a real gene or should we un-gene it? *F > *F >Any information you have would be helpful, and will be cited in FlyBase *F >as a personal communication from you. *F > *F >Thanks in advance for your help, *F > *F >Bill *F > *F > *F >*a uns *F >*e unstrung *F >*z FBgn0005766 *F >*b 2-22 *F >*c 27A *F >*x FBrf0053298 == ew981 == Musacchio and Perrimon, 1991, Greenspan, Palka, *F >1991: 123 *F >*E FBrf0053298 == ew981 == Musacchio and Perrimon, 1991, Greenspan, Palka, *F >1991: 123 *F >*p Mutants of @uns@ exhibit missing intersegmental longitudinal tracts. # *U FBrf0104602 *a Montell *b D. *t 1998.9.9 *T personal communication to FlyBase *u *F From Denise_Montell@bs.jhmi.edu Wed Sep 09 15:37:26 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 9 Sep 1998 15:37:26 +0100 *F Date: 9 Sep 1998 10:31:27 -0400 *F From: 'Denise Montell' *F Subject: RhoL and DRac3 *F To: 'flybase' *F X-Mailer: Mail*Link SMTP-QM 4.1.0 *F Content-Length: 635 *F To whom it may concern, *F We have become aware of an error in Flybase that is due to our own mistake and *F we would like to correct it to prevent confusion. The gene known as RhoL, *F which we cloned and published (Murphy and Montell, JCB 1996, v. 133, *F pp617-630) was incorrectly mapped to 67C on polytene chromosomes. This gene *F is identical in sequence to a gene that has been named Rac3 and which has been *F localized to 85D. We believe that the 85D localization is correct and the 67C *F localization is incorrect. We apologize for the error and hope that you can *F correct the Flybase entries as soon as possible. *F Sincerely, *F Denise Montell # *U FBrf0104709 *a Haenlin *b M. *t 1998.9.18 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Mon Sep 07 11:06:27 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 7 Sep 1998 11:06:27 +0100 *F To: haenlin@cict.fr *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 7 Sep 1998 11:06:37 +0100 *F Content-Length: 1350 *F Dear Dr. Haenlin, *F I am curating a paper for FlyBase (Baker and Yu, 1998, Mech. Dev. 74(1,2): *F 3--14) in which a Dl-enhancer trap construct isolated by you and J.A. *F Campos-Ortega was used. *F FlyBase has a record of a number of Dl-enhancer trap constructs but I don't *F think that that these correspond to your construct. I wrote to the *F corresponding author on the paper (N. Baker) and he suggested I write to *F you about the details of the construct. *F I would be grateful if you could tell me a few details of the construct so *F that I can confirm whether this construct is new to FlyBase. If it is new *F to FlyBase I would like to include the details in FlyBase as a personal *F communication from you, if possible. *F 1. is there a reference where this construct has been published. *F 2. is there a designation/line number for the construct e.g. A101, 64B *F 3. which enhancer trap vector, e.g. P{PZ}, P{lacW} is used *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From haenlin@cict.fr Thu Sep 17 18:37:21 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Sep 1998 18:37:21 +0100 *F X-Sender: haenlin@mail.cict.fr *F Mime-Version: 1.0 *F X-Mailer: Eudora F3.0 Demo *F Date: Thu, 17 Sep 1998 19:34:25 +0200 *F To: Gillian Millburn (Genetics) *F From: Marc Haenlin *F Subject: Re: FlyBase query *F >Dear Dr. Haenlin, *F > *F >I am curating a paper for FlyBase (Baker and Yu, 1998, Mech. Dev. 74(1,2): *F >3--14) in which a Dl-enhancer trap construct isolated by you and J.A. *F >Campos-Ortega was used. *F > *F >FlyBase has a record of a number of Dl-enhancer trap constructs but I don't *F >think that that these correspond to your construct. I wrote to the *F >corresponding author on the paper (N. Baker) and he suggested I write to *F >you about the details of the construct. *F > *F >I would be grateful if you could tell me a few details of the construct so *F >that I can confirm whether this construct is new to FlyBase. If it is new *F >to FlyBase I would like to include the details in FlyBase as a personal *F >communication from you, if possible. *F > *F >1. is there a reference where this construct has been published. *F >2. is there a designation/line number for the construct e.g. A101, 64B *F >3. which enhancer trap vector, e.g. P{PZ}, P{lacW} is used *F > *F Dear Gillian *F I sendet two lines; both of them are insertion of P{lacW} in the promotor *F region of Delta at about the same site (less as 1 kb). *F For the 8c3 (originating from the Jan's Lab as I know) the LacZ *F transcription orientation is the same as Delta (the Dl revF10 is a *F revertant of this enhancer trap line), and for the 1282 (from Seugnet and *F Haenlin) is in the opposite direction. *F It exist as I know no references for these lines. *F If you have further questions, no problem *F regards *F Haenlin Marc *F Centre de Biologie du Developpement *F Batiment 4R3 *F 118, route de Narbonne *F 31062 Toulouse *F Tel: (33) 5.61.55.82.85 *F Fax : (33) 5.61.55.65.07 *F From gm119@gen.cam.ac.uk Fri Sep 18 10:11:39 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 18 Sep 1998 10:11:39 +0100 *F To: haenlin@cict.fr *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 18 Sep 1998 10:12:03 +0100 *F Content-Length: 440 *F Dear Marc, *F thankyou for your reply, it was very helpful. *F I think I have found the 8c3 line in FlyBase - we have a P{lacW} insertion *F in Dl called j8C3 which comes from the Berkeley Genome Project - and I *F think the j means that it came from the Jan's lab. *F the 1282 line is new. *F I would like to include the details from your e-mail about these lines in *F FlyBase as a personal communication to FlyBase from you. *F Would that be OK ? *F Gillian *F From haenlin@cict.fr Fri Sep 18 11:36:29 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 18 Sep 1998 11:36:29 +0100 *F X-Sender: haenlin@mail.cict.fr *F Mime-Version: 1.0 *F X-Mailer: Eudora F3.0 Demo *F Date: Fri, 18 Sep 1998 12:33:36 +0200 *F To: Gillian Millburn (Genetics) *F From: Marc Haenlin *F Subject: Re: FlyBase query *F >Dear Marc, *F > *F >thankyou for your reply, it was very helpful. *F > *F >I think I have found the 8c3 line in FlyBase - we have a P{lacW} insertion *F >in Dl called j8C3 which comes from the Berkeley Genome Project - and I *F >think the j means that it came from the Jan's lab. *F > *F >the 1282 line is new. *F > *F >I would like to include the details from your e-mail about these lines in *F >FlyBase as a personal communication to FlyBase from you. *F > *F >Would that be OK ? *F > *F Dear Gillian *F no problem # *U FBrf0104710 *a Matthews *b K. *t 1998.8.27 *T personal communication to FlyBase *u *F Personal communication from: Kathy Matthews, Indiana University *F Subject: l(3)85E[1] *F Dated: 27 Aug 1998 *F *F Background: 'new' gene in 85E *F *F Gene symbol ms(3)85E *F Full name male sterile (3) 85E *F Allele symbol ms(3)85E[1] *F Phenotype male sterile *F Cyto. loc. 85E1--85E10 *F Df. comp. Included in Df(3R)by10, Df(3R)GB104, Df(3R)by62 *F Not included in Df(3R)by416 *F Other comp. Complements for viability and male fertility: hyd, knk, *F l(3)85Eb, l(3)85Ec, l(3)85Ed, l(3)85Ee, and Scm *F Synonym ms(3)KM27 *F Discoverer K. Matthews # *U FBrf0104711 *a Bloomington Drosophila Stock Center *b ?. *t 1998.8.25 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Variants of Payne *F Date: 25 August 1998 *F *F Background: Balancer variants in the Bloomington collection that are not in FlyBase. *F *F Information communicated: *F *F Payne, Dfd[1] *F Payne, Dfd[1] ca[1] *F Payne, Dfd[1] Sb[1] *F Payne, Me[1] # *U FBrf0104712 *a Bloomington Drosophila Stock Center *b ?. *t 1998.8.28 *T personal communication to FlyBase *u *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: F(3R)4, l(2)22Fc[H7] and l(2)35Fb[ck1] *F Date: 28 August 1998 *F *F Background: Aberrations and alleles not in FlyBase *F *F Information communicated: *F *F Aberration F(3R)4, se[1] e[s] *F Discoverer E.H. Grell *F *F Allele l(2)22Fc[H7] *F Per Hugo Bellen *F *F Allele l(2)35Fb[ck1] *F Per John Roote, 20 December 1997 # *U FBrf0104713 *a Botas *b J. *t 1998.8.26 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Juan Botas, Baylor College of Medicine *F To: Bloomington Drosophila Stock Center *F Subject: Df(2R)B80 *F Dated: 26 August 1998 *F *F Information communicated: *F *F Df(2R)B80 deletes or disrupts Kr. # *U FBrf0104714 *a Rawls *b J. *t 1998.7.3 *T personal communication to FlyBase *u *F Four alleles for four lethal complementation groups in cytogenetic region *F 93B have been sent. Each was isolated by Teresa Vincent and was reported in *F Molec. Gen. Genet. 222:1-8, 1990. Listed below are the names of these genes *F according to the current Flybase name. Following each, in <>, is given the *F gene name used in the original publication. *F l(3)93Bc1 eLE1 cd / TM3, ri pp Sb Ser e *F l(3)93Bd1 eLE1 cd / TM3, ri pp Sb Ser e *F l(3)93Bg1 eLE1 cd / TM3, ri pp Sb Ser e *F l(3)93Bi1 eLE1 cd / TM3, ri pp Sb Ser e # *U FBrf0104715 *a Gelbart *b W.M. *c D. *d Smith *e J. *f Wohlgemuth *t 1998.9.3 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Thu Sep 03 17:19:52 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Re: questionable LanA allele *F dpp151H: This insertion is molecularly into the dpp disk region (3' untranscribed, enhancer containing region of the gene, you might recall). However, it is phenotypically completely wild-type. # *U FBrf0104716 *a Grushko *b T. *c J. *d Vilkki *e H. *f Heikanen *g P. *h Portin *t 1998.9.7 *T personal communication to FlyBase *u *F Archived. # *U FBrf0104718 *a Roote *b J. *t 1998.8.26 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: John Roote, University of Cambridge *F To: Bloomington Drosophila Stock Center *F Subject: Variant of CyO *F Dated: 26 August 1998 *F *F Information communicated: *F *F Balancer variant CyO, b[81f2] rk[81f2] *F Discoverer David Gubb # *U FBrf0104719 *a Bloomington Drosophila Stock Center *b ?. *t 1998.9.14 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Alleles, transposons and insertions not in FlyBase *F Date: 14 September 1998 *F *F Background: An assortment of genes, alleles and transposon insertions at Bloomington but not in FlyBase are listed. *F *F Information communicated: *F *F Gene-alleles *F M(3)40l30[1] -- letter l, not number 1, in gene symbol; this is a Minute attributed to Ed Lewis. *F ms(3)nc14[1] -- nc stands for noncomplementer; this EMS-induced male sterile allele fails to complement recessive betaTub85D alleles but it does not map to that locus. Made by Ken Kemphues. *F ms(3)ry1[1] -- associated with the (unlocalized) insertion of P{ry11}, to us from Allan Spradling. *F ms(3)ry3[1] -- associated with the (unlocalized) insertion of P{ry11}, to us from Allan Spradling. *F Dsim\y[K] -- to us from John Roote of the Ashburner lab. This allele arose (was induced?) in the D. simulans wild-type strain C167.4. Fertile hybrid females are produced from crosses of Dsim\y[K] females to D. melanogaster In(1)AB males. *F *F Transposon insertions *F P{Acp70A[g.Yp1.hs]}G10 -- location of insertion unknown. Induces ovulation in virgin females; useful for recovering large numbers of unfertilized eggs. From Eric Kubli. *F P{Act5C-GAL4}17bFO1 -- insertion on chromosome 3. From Yash Hiromi. *F P{Act5C-GAL4}25FO1 -- insertion on chromosome 2. From Yash Hiromi. *F P{AyGAL4}25 -- insertion on chromosome 2. From Yash Hiromi. Might be the same as P{AyGAL4}2nd.I, but 25 is the insertion identifier used by the donor of the stock. *F P{HS:Ubx-Ia}22 -- insertion on chromosome 3. From Richard Mann. *F P{lacW}64A -- insertion in 64A. lacZ expressed in sensory organ precursor cells and PNS. From Hugo Bellen. *F P{GawB}c49 -- Unlocalized insertion. GAL4 expression pattern in oocyte: follicle cells strong at posterior pole, graded anteriorly. From Lynn Manseau. *F P{lacW}k13720 -- l(3)k13720 is unverified. The chromosome is therefore represented as P{lacW}k13720, l(3)k13720[k13720]. *F P{lacW}k07118 -- l(3)k07118 is unverified. The chromosome is therefore represented as P{lacW}k07118, l(3)k07118[k07118]. *F *F Transposons and insertions *F P{NGT}40 -- nanos promoter driving GAL4, 3' UTR from a maternally expressed alpha-tubulin, in pCaSpeR. Insertion on chromosome 2. From Peter Gergen. Note from KM: although he didn't specify, the tubulin is probably alphaTub67C, the only one that is exclusively maternal. *F P{UAS-NZ}20b -- SV40 T NLS-lacZ fusion in pUAST (beta-gal is localized to the nucleus). Insertion on chromosome 2. From Yash Hiromi and S. West, unpublished. *F P{UAS-NZ}J312 -- Insertion on chromosome 3. From Yash Hiromi and S. West, unpublished. *F P{prd-GAL4}RG1 -- References: FBrf0064375 and FBrf0083528. Insertion on chromosome 3. GAL4 expressed in a prd pattern. *F *F Synonyms *F Oab[1] is a synonym for Oab[SCG1] *F Opl[1] is a synonym for Opl[SC1] *F Roa[1] is a synonym for Roa[SCG1] # *U FBrf0104720 *a Littleton *b T. *t 1998.9.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Sep 16 09:36:07 1998 *F To: tjlittle@facstaff.wisc.edu *F Subject: Helping FlyBase *F Dear Troy, *F I am curating your Neuron paper for FlyBase: *F Temperature sensitive paralytic mutations demonstrate that synaptic *F exocytosis requires SNARE complex assembly and disassembly *F Neuron 21: 401-413 *F and I have a question for you. Figure 1 shows the effect of rescuing *F 'ts3-69' with a wild type copy of the syx1A gene. However you do not *F describe or give a reference for the wild type syx1A transgene. *F Is it this one? *F \*A syx1A+t6 *F \*z FBal0056009 *F \*x FBrf0091165 == Schulze and Bellen, 1996, Genetics 144(4): 1713--1724 *F \*E FBrf0091165 == Schulze and Bellen, 1996, Genetics 144(4): 1713--1724 *F \*i unnamed *F \*o in vitro construct | regulatory fusion *F \*I P{syx+6} *F \*k Mode of assay: In transgenic Drosophila *F \*Q Rescues: syx1A06737 *F \*Q Rescues: syx1A&Dgr;229 *F \*Q Lethality of @syx1A06737@ and @syx1A&Dgr;229@ is rescued. *F \*s 6.0kb NotI fragment encompassing the @syx1A@ open reading frame and *F \*s up and down sequences. *F Many thanks for your help, *F best wishes, *F Rachel. *F From tjlittle@facstaff.wisc.edu Wed Sep 16 16:58:51 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase *F Dear Rachel, *F The syntaxin rescue construct is actually P{syx+13}, but it encompasses *F the same syx1A reading frame as Psyx6. *F Thanks, Troy # *U FBrf0104722 *a Thummel *b C.S. *t 1998.8.31 *T personal communication to FlyBase *u *F Personal communication from: Carl Thummel, University of Utah *F To: Bloomington Drosophila Stock Center *F Subject: Ubiquitin-GFP-marked Balancers *F Dated: 31 August 1998 *F Information communicated: *F The ubiquitin-GFP-marked CyO and TM6B balancer stocks donated to the *F Bloomington Stock Center carry pPwum2. The ref is: Heck et al. (1993) *F 123:665-679. The GFP.S65T gene has been inserted into this vector. *F This was then used to transform flies (by J. Qiu in Kalpana White's *F lab) and then we (Paulo D'Avino in my lab) hopped it onto balancers. # *U FBrf0104838 *a Heilig *b J.S. *t 1998.10.3 *T personal communication to FlyBase *u *F >From heilig@samiam.colorado.edu Thu Sep 03 21:16:13 1998 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 3 Sep 1998 21:16:13 +0100 *F Date: Thu, 3 Sep 1998 14:16:18 -0600 (MDT) *F From: HEILIG JOSEPH S *F To: Michael Ashburner *F Subject: Re: can we beat your tric ? *F MIME-Version: 1.0 *F Dear Michael, *F I remain extremely certain that tric is beat. This is based on *F indistinguishable Bolwig Organ defects in our tric allele and Corey's beat *F alleles. Our allele does not affect the motoneurons, however, so *F reciprocity is not maintained. UAS-beat directed to the Bolwig Organs or *F optic lobes rescues the BO phenotype of tric and beat mutants. Reduction *F of Fas II expression rescues the BO phenotype too, just as it does in the *F motoneurons. tric and beat alleles fail to complement when Bolwig Organ *F phenotype is assessed. So, by those criteria they look pretty much the same. We *F sequenced the coding region of beat from our tric allele and found no *F mutations. Corey's group did not sequence their beat alleles. So, by *F most people's standards I think tric=beat and we do conclude *F that tric and beat are the same in a MS that we have submitted. *F Regards, *F Joe # *U FBrf0104845 *a Pointud *b J.C. *t 1998.9.23 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Sep 17 13:08:32 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Sep 1998 13:08:32 +0100 *F To: J-Christophe.POINTUD@inserm.u-clermont1.fr *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 17 Sep 1998 13:08:49 +0100 *F Content-Length: 1369 *F Dear Dr. Pointud, *F I am curating your abstract for FlyBase: *F Pointud et al., 1998, Int. J. Dev. Biol. 42(6): 29S *F 'Function of the bric a brac genes during adult ovary and leg *F morphogenesis.' *F in which you describe the isolation of two genes: bab-I and bab-II. *F At the moment FlyBase only has a record of one bab (bric a brac) gene. I *F would like to confirm whether either/both of your bab genes is the same as *F the bab already in FlyBase. Is the situation like polyhomeotic, where *F there are two duplicated copies of the polyhomoetic gene, ph-d (distal) and *F ph-p (proximal) ? If this is the case, I would be grateful for any *F information you may have about the molecular nature of the bab mutations *F already in FlyBase - do you know whether they are in bab-I or bab-II. I *F include a list of the bab mutations below ( = superscript), *F babE1 *F babP *F babPR10 *F babPR11 *F babPR23 *F babPR24 *F babPR30 *F babPR72 *F babPRDS *F I look forward to hearing from you, *F Gillian Millburn *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From J-Christophe.POINTUD@u-clermont1.fr Wed Sep 23 10:36:51 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 23 Sep 1998 10:36:51 +0100 *F X-Sender: pointud@mailhost.u-clermont1.fr *F X-Mailer: QUALCOMM Windows Eudora Light Version 3.0.5 (32) *F Date: Wed, 23 Sep 1998 11:31:37 +0200 *F To: Gillian Millburn (Genetics) *F From: Jean-Christophe Pointud *F Subject: Re: FlyBase query *F Mime-Version: 1.0 *F Content-Transfer-Encoding: quoted-printable *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Length: 411 *F Dear Dr. Millburn, *F The bab gene recorded in Flybase is the bab-I gene. *F The bab-II gene is at the same cytological location (61F) but is *F MOLECULARLY DIFFERENT of the bab-I gene. *F All the bab mutations in Flybase are in the bab-I gene. *F Sincerely yours, *F POINTUD Jean-christophe *F Unité INSERM U384 *F faculté de médecine *F 28, place Henri Dunant *F 63001 CLERMONT-FERRAND *F tel 04 73 60 80 24 *F fax 04 73 27 61 32 # *U FBrf0104846 *a Doane *b W.W. *t 1996.8.2 *T personal communication to FlyBase *u *F >From icwwd@asu.edu Fri Aug 2 01:28:46 1996 *F Date: Thu, 01 Aug 1996 17:02:57 -0700 (MST) *F From: 'Winifred W. Doane' *F Subject: Correction of error in FlyBase *F To: Genetic map position of adp *F Content-Transfer-Encoding: 7BIT *F Content-Length: 778 *F X-Lines: 17 *F Status: RO *F The genetic map position of the adipose (adp) locus remains 2-83.4, as *F originally published. An unfortunate typographical error appears in *F FBrf0072971 (Doane, W. W. 1994 DIS, 75:168), namely, the last two *F numbers were inverted so that 2-84.3 appeared in print. This error was *F duplicated in FBrf0073081 (Foehr, E. D. and Doane, W. W. 1994 DIS, 75: *F 168-169). The genetic data from this laboratory all indicates the adp *F locus is at 2-83.4, so the erroneous location noted should be removed *F from the FlyBase Gene Report and all other parts of FlyBase. *F With humble apologies, *F Winifred W. Doane *F Winifred W. Doane *F Department of Zoology *F Arizona State University *F Tempe, AZ 852870-1501 TEL: (602) 965-7189 *F e-mail: icwwd@asuvm.inre.asu.edu FAX: (602) 965-2519 # *U FBrf0104865 *a Georgiev *b P. *t 1998.9.28 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Sep 25 13:52:02 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 25 Sep 1998 13:52:02 +0100 *F To: pgeorg@biogen.msk.su *F Subject: Helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Fri, 25 Sep 1998 13:52:38 +0100 *F Content-Length: 2752 *F Dear Dr. Georgiev, *F I am writing about some alleles in a recent paper of yours: *F Belenkaya et al., 1998 *F P element sequences can compensate for a deletion in the yellow regulatory *F region in Drosophila melanogaster. *F Mol Gen Genet 259: 79-87. *F In your paper, on page 84, you discuss y+s2, a revertant of one of the *F 'y2s' alleles. We would like to make a formal link between this allele *F and its parental allele, but since you do not specify which particular *F 'y2s' allele is its parent we cannot. Please could you tell us the *F particular progenitor for each of y+s2, y+s3, y+s6, y+s7 and *F y+s8? *F With best wishes, *F looking forward to hearing from you, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From pgeorg@biogen.msk.su Mon Sep 28 07:42:51 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 28 Sep 1998 07:42:51 +0100 *F To: rd120@gen.cam.ac.uk *F Organization: Institute for Gene Biology of RAS *F From: 'Pavel G.Georgiev' *F Date: Mon, 28 Sep 98 06:22:26 +0400 *F X-Mailer: BML MS/DOS Beauty Mail v.1.36 *F Subject: Re: Helping FlyBase *F Lines: 24 *F Content-Length: 1186 *F Dear Rachel, *F The y+s2, y+s7 and y+s8 alleles were originated from *F the y2s14 allele. The y+s3 and y+s6 alleles were originated *F from the y2sA3. *F With best wishes, *F Pavel *F From rd120@gen.cam.ac.uk Mon Sep 28 09:35:15 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 28 Sep 1998 09:35:15 +0100 *F To: pgeorg@biogen.msk.su *F Subject: Re: Helping FlyBase *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 28 Sep 1998 09:35:48 +0100 *F Content-Length: 1364 *F Dear Pavel, *F Thank you for your reply. *F >The y+s2, y+s7 and y+s8 alleles were originated from *F >the y2s14 allele. The y+s3 and y+s6 alleles were originated *F >from the y2sA3. *F Marvellous. We will record this information as a personal communication *F from you. *F Many thanks for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0104866 *a Jacobs *b H. *t 1998.9.29 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Thu Oct 01 16:50:30 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Oct 1998 16:50:30 +0100 *F To: gm119@gen.cam.ac.uk *F Subject: A2(2nd)27 *F Cc: ag24@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Aubrey de Grey *F Date: Thu, 1 Oct 1998 16:51:10 +0100 *F Content-Length: 516 *F Dear Christian, *F We have just received the GenBank sequences AJ006207 and AJ006208, *F submitted by your group, which are described as flanking A2(2nd)27. *F We do not have a record of that insertion causing a mutation in *F any gene, but I see that the unpublished reference included in the *F Genbank records mentions blw in the title. It would be very helpful *F for our data integrity if you could tell me whether A2(2nd)27 is in *F fact an allele of blw (or of any other gene). *F Many thanks in advance, *F Aubrey de Grey *F FlyBase *F From Henning.Jacobs@uni-bayreuth.de Tue Sep 29 12:31:55 1998 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 29 Sep 1998 12:31:55 +0100 *F Date: Tue, 29 Sep 98 13:33:50 +0200 *F X-Sender: btb712@btr0x1.rz.uni-bayreuth.de *F Mime-Version: 1.0 *F To: ag24@gen.cam.ac.uk *F From: Henning.Jacobs@uni-bayreuth.de (Henning Jacobs) *F Subject: A2(2nd)27 *F Dear Dr. de Grey, *F I am a PhD-student in Christian Lehners Lab. Christian asked me to answer *F your mail about the A2(2nd)27 P-element insertion. This insertion indeed is *F an allele of blw, according to complementation tests that we have performed *F using lethal EMS-alleles (from a screen we have done in the 59AB-region) *F and various P-element lines including the original blw-allele blw(1). *F A(2)2nd27 and blw(1) are male sterile over themselves, over Df(2R)59AB and *F over two out of eleven lethal EMS-alleles comprising one of four *F complementation groups from our screen. (We just tested only two of the *F eleven alleles for complementation with the P-insertions). According to *F sequence comparisons, A(2)2nd27 seems to reside in the 5'-UTR of the gene *F encoding the alpha subunit of the mitochondrial ATP-synthase of Drosophila. *F (The reference included in the Genbank-records is in press meanwhile and *F will soon be published in 'Molecular and General Genetics'). *F I hope this information will be of some help, *F best regards, *F Henning Jacobs *F Henning Jacobs *F Department of Genetics *F Lehner-Lab *F University of Bayreuth *F D-95440 Bayreuth *F Germany *F phone: +49 921 55 2707 *F fax: +49 921 55 2710 *F e-mail: henning.jacobs@uni-bayreuth.de *F From gm119@gen.cam.ac.uk Thu Oct 01 17:25:04 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Oct 1998 17:25:04 +0100 *F To: Henning.Jacobs@uni-bayreuth.de *F Subject: Re: A2(2nd)27 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 1 Oct 1998 17:25:44 +0100 *F Content-Length: 2981 *F Dear Henning, *F thankyou for the information about the A2(2nd)27 insertion. Aubrey has *F given me your e-mail to curate as a personal communication to FlyBase from *F you - is that OK ? *F Also, at the end of your e-mail you say: *F 'A(2)2nd27 seems to reside in the 5'-UTR of the gene encoding the alpha *F subunit of the mitochondrial ATP-synthase of Drosophila' *F FlyBase has a record of a gene with the symbol ATPsyn-alpha, which is *F stated to encode the mitochondrial ATP synthetase alpha chain. The *F information we have about this gene is: *F Accession numbers: AA801788, Y07894, Q94512, P35381. *F References: *F Santaren et al., 1993, Exp. Cell Res. 206(2): 220--226 *F Talamillo et al., 1998, Molec. Biol. Rep. 25(2): 87--94 *F Do you know whether this ATPsyn-alpha is the same as the one encoded by *F blw? If it is, I can merge the 2 genes, keeping blw as the valid symbol, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F > From Henning.Jacobs@uni-bayreuth.de Tue Sep 29 12:31:55 1998 *F > Envelope-to: ag24@gen.cam.ac.uk *F > Delivery-date: Tue, 29 Sep 1998 12:31:55 +0100 *F > Date: Tue, 29 Sep 98 13:33:50 +0200 *F > X-Sender: btb712@btr0x1.rz.uni-bayreuth.de *F > Mime-Version: 1.0 *F > To: ag24@gen.cam.ac.uk *F > From: Henning.Jacobs@uni-bayreuth.de (Henning Jacobs) *F > Subject: A2(2nd)27 *F > *F > Dear Dr. de Grey, *F > *F > I am a PhD-student in Christian Lehners Lab. Christian asked me to answer *F > your mail about the A2(2nd)27 P-element insertion. This insertion indeed is *F > an allele of blw, according to complementation tests that we have performed *F > using lethal EMS-alleles (from a screen we have done in the 59AB-region) *F > and various P-element lines including the original blw-allele blw(1). *F > A(2)2nd27 and blw(1) are male sterile over themselves, over Df(2R)59AB and *F > over two out of eleven lethal EMS-alleles comprising one of four *F > complementation groups from our screen. (We just tested only two of the *F > eleven alleles for complementation with the P-insertions). According to *F > sequence comparisons, A(2)2nd27 seems to reside in the 5'-UTR of the gene *F > encoding the alpha subunit of the mitochondrial ATP-synthase of Drosophila. *F > (The reference included in the Genbank-records is in press meanwhile and *F > will soon be published in 'Molecular and General Genetics'). *F > *F > I hope this information will be of some help, *F > best regards, *F > *F > Henning Jacobs *F > *F > Henning Jacobs *F > Department of Genetics *F > Lehner-Lab *F > University of Bayreuth *F > D-95440 Bayreuth *F > Germany *F > *F > phone: +49 921 55 2707 *F > fax: +49 921 55 2710 *F > e-mail: henning.jacobs@uni-bayreuth.de *F > *F > *F > *F > *F > ----- End Included Message ----- *F > *F From Henning.Jacobs@uni-bayreuth.de Thu Oct 01 22:42:41 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Oct 1998 22:42:41 +0100 *F Date: Thu, 1 Oct 98 23:45:17 +0200 *F X-Sender: btb712@btr0x1.rz.uni-bayreuth.de *F Mime-Version: 1.0 *F To: Gillian Millburn (Genetics) *F From: Henning.Jacobs@uni-bayreuth.de (Henning Jacobs) *F Subject: Re: A2(2nd)27 *F >Dear Henning, *F > *F >thankyou for the information about the A2(2nd)27 insertion. Aubrey has *F >given me your e-mail to curate as a personal communication to FlyBase from *F >you - is that OK ? *F > *F >Also, at the end of your e-mail you say: *F > *F >'A(2)2nd27 seems to reside in the 5'-UTR of the gene encoding the alpha *F >subunit of the mitochondrial ATP-synthase of Drosophila' *F > *F >FlyBase has a record of a gene with the symbol ATPsyn-alpha, which is *F >stated to encode the mitochondrial ATP synthetase alpha chain. The *F >information we have about this gene is: *F > *F >Accession numbers: AA801788, Y07894, Q94512, P35381. *F > *F >References: *F > *F >Santaren et al., 1993, Exp. Cell Res. 206(2): 220--226 *F >Talamillo et al., 1998, Molec. Biol. Rep. 25(2): 87--94 *F > *F >Do you know whether this ATPsyn-alpha is the same as the one encoded by *F >blw? If it is, I can merge the 2 genes, keeping blw as the valid symbol, *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F Dear Gillian, *F \- To curate the information as a personal communication to FlyBase is o.k. *F \- The ATPsyn-alpha gene (accession number Y07894) described in Talamillo et *F al., 1998 indeed is the same gene. *F I hope this information is of some help. *F Henning # *U FBrf0104930 *a Gubb *b D. *t 1998.9.17 *T personal communication to FlyBase *u *F From dg27@mole.bio.cam.ac.uk Thu Sep 17 09:36:54 1998 *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F I have put everything back together again, which I suppose is one *F up on all the Kings horses etc. XX David *F The latest two Cam Dps now have stock numbers and appear on our FlyBase *F stock list: *F 4744 In(2LR)DTD99[L]RevB[R], dpp[d-ho] cn[1] bw[1]/CyO = Dp(2;2)Cam17 *F 4745 In(2LR)Pu[LyL]DTD99[R]/CyO = Dp(2;2)Cam18 *F I want to be sure all of the stocks are in good shape before I send out the *F announcement. I will temporarily expand the stocks, since we usually get a *F burst of orders whenever we post one of these announcements on *F bionet.drosophila. *F Look for the 'advertisement' in 2 to 3 weeks. *F Thanks for the stocks and helpful comments *F Kevin *F >The Cam Duplication Kit *F > *F >A set of large duplications covering >90% of the second chromosome is now *F >available from the Bloomington Drosophila Stock Center. These Dp(2;2) *F >stocks were generated and donated by David Gubb, Darin Coulson, Siegrun *F >Herrmann and John Roote of Cambridge University. These duplications are *F >useful in screening for dosage-dependent enhancers and suppressors of *F >mutant phenotypes. In addition, because the CAM duplications are nested *F >within inverted chromosomal segments, they act as balancer chromosomes for *F >the duplicated regions. This makes the CAM duplications ideal for *F >recovering (and maintaining) haplo-lethal, or haplo-sterile, mutations. *F > *F >Short name Stock # Duplicated region *F > *F >Dp(2;2)Cam1 3392 21E2 to 23D1 *F >Dp(2;2)Cam2 3394 23D1 to 26C1-2 *F >Dp(2;2)Cam3 3401 26C1-2 to 29E *F >Dp(2;2)Cam4 3403 29E to 32F *F >Dp(2;2)Cam5 4521 32F to 35B1-2 *F >Dp(2;2)Cam6 4518 35B1-2 to 36C *F >Dp(2;2)Cam8 2630 36C to 2Lh *F >Dp(2;2)Cam10 4520 2Rh to 43A1-2 *F >Dp(2;2)Cam11 2626 43A1-2 to 47B10-14 *F >Dp(2;2)Cam13 3391 47B10-14 to 48C *F >Dp(2;3)Cam14T 4519 49A to 51EF *F >Dp(2;2)Cam17 4744 52D10-E1 to 53F *F >Dp(2;2)Cam18 4745 53F to 57C4-6 *F >Dp(2;2)Cam16 2622 57C4-6 to 60E5-8> *F >The genotypes of these duplication stocks are given below as they appear in *F >the Bloomington stock list. (Note that the 'Dp(2;2)Cam' chromosome names *F >are used for convenience. The full designations of these aberrations *F >reflect their construction from recombinants between pairs of pericentric *F >inversions. *F > *F >3392 In(2LR)DTD8[L]S[325R]/CyO, bw[1] = Dp(2;2)Cam1 *F >3394 In(2LR)DTD24[L]DTD8[R], sp[1]/CyO, bw[1] = Dp(2;2)Cam2 *F >3401 In(2LR)DTD111[L]DTD24[R], bw[1] sp[1]/CyO, bw[1] = Dp(2;2)Cam3 *F >3403 In(2LR)DTD107[L]DTD111[R], Dp(1;2)TE23CD, vg[1]/CyO, bw[1] = *F >Dp(2;2)Cam4 *F >4518 In(2LR)S1[L]noc[4R], cn[1] bw[1]/CyO = Dp(2;2)Cam6 *F >4521 In(2LR)noc[4L]DTD107[R], bw[1] sp[1]/In(2LR)Gla, Gla[1] Bc[1] Egfr[E1] *F >ap[*] cn[1] bw[1] = Dp(2;2)Cam5 *F >2630 In(2LR)C3[L]CH9-25[R], b[1]/In(2L)Cy, In(2R)Cy, al[2] Cy[1] pr[1] *F >Bl[1] cn[2] vg[1] c[1] sp[2] = Dp(2;2)Cam8 *F >4520 In(2LR)TE35B-SZ4[L]noc[4R], (al[1]) dp[ov1] b[1] noc[TE35B] pr[1] *F >cn[1] bw[1]/CyO, Df(2L)noc20 = Dp(2;2)Cam10 *F >2626 (y[*] w[*]); In(2LR)TE35B-226[L]TE35B-4[R], b[1] pr[1] pwn[1]/CyO, *F >b[1] rk[1] = Dp(2;2)Cam11 *F >3391 In(2LR)DTD128[L]TE35B-226[R], Dp(1;2)TE23CD, net[1] dpp[d-ho]/CyO = *F >Dp(2;2)Cam13 *F >4519 Ts(2Lt;3Rt)6r23+Ts(2Rt;3Lt)H24, cn[1]/CyO = Dp(2;3)Cam14T (NB: *F >translocation segregants) *F 4744 In(2LR)DTD99[L]RevB[R], dpp[d-ho] cn[1] bw[1]/CyO = Dp(2;2)Cam17 *F 4745 In(2LR)Pu[LyL]DTD99[R]/CyO = Dp(2;2)Cam18 *F >2622 In(2LR)lt[G16L]Pu[LyR], b[1] bw[1]/CyO = Dp(2;2)Cam16 *F > *F >This list of stocks is also available from *F >http://flybase.bio.indiana.edu/stocks/stock-centers/bloomington/lk/cam-dp-kit # *U FBrf0104931 *a Bloomington Drosophila Stock Center *b ?. *t 1998.9.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Sep 22 19:31:28 1998 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Hi folks-- *F We recently received a set of stocks from Rod Nagoshi that had been *F generated in Dawson Mohler's lab. Among the stocks were two alleles of *F fs(2)A12 that were described in Bakken, Developmental Biology 33: 100-122, *F 1973. The stocks are: *F 4571 fs(2)A126-396/SM1 *F 4572 fs(2)A128-1062/SM1 *F I took a look at the ovaries of homozygotes and 6-396/8-1062 females *F and they have a classic dumpless phenotype. My complementation tests *F indicate that fs(2)A12 is the same as quail. fs(2)A128-1062 failed to *F complement qua1 and Df(2L)TW137 (Df for quail and kelch), but *F complemented Df(2L)M36F-S5 (Df for kelch) and Df(2L)GpdhA (Df for *F chickadee). *F Consequently, could you do your curatorial magic on the fs(2)A12 *F (FBgn0000942) and quail (FBgn0003187) entries? What do you want to name *F the new alleles? qua6-396 and qua8-1062? *F Best regards, *F Kevin # *U FBrf0104932 *a Bloomington Drosophila Stock Center *b ?. *t 1998.9.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Sep 23 21:05:56 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 23 Sep 1998 21:05:56 +0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: Windows Eudora Light Version 3.0.1 (32) *F Date: Wed, 23 Sep 1998 15:04:31 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: VA228 *F Mime-Version: 1.0 *F To: FlyBase updates *F From: Kevin Cook at the Bloomington Drosophila Stock Center (9/23/98) *F We recently acquired George Lefevre's line VA228 from Norbert Perrimon. *F VA228 was listed as both uncl10 and stn10 in Lindsley and Zimm. They *F cited Lefevre and Watkins (Genetics 113: 869-895, 1986) as the source of *F this information. This citation was used as a catch-all reference for *F information about Lefevre lethals that really came from correspondence with *F Lefevre. VA228 was never mentioned by name in the paper. *F Currently, FlyBase lists VA228 as a synonym for uncl10. It is not given *F as a synonym for stnA10, even though the mutation is shown originating *F with Lefevre. *F In Perrimon et al. (Genetics 121: 313--331, 1989) where lethals at the base *F of the X chromosome were described, VA228 was given as an allele of uncl, *F but not stn. Norbert Perrimon sent the stock as uncl10 and knew nothing *F about a stnA mutation. *F I crossed VA228/FM7 females to stnA1/Dp(1:Y)y+mal106 males and *F VA228/stnA1 females were viable. They showed no bang-sensitivity, *F paralysis or uncoordination at room temperature or after an hour at 29 *F degrees. *F uncl and stnA are tightly linked and the VA228 stock was maintained by *F Perrimon as it was received from Lefevre. No attempt was ever made to *F isolate a uncl mutation away from other linked lethals. *F I suspect that stnA10 never existed and that there was some problem *F incorporating Lefevre's notes into Lindsley and Zimm. The phenotypes of *F uncl and stnA are similar enough to cause confusion. We have chosen to *F list VA228 as uncl10/FM7 (stock 4734) in our stock list. *F I believe that Lefevre's correspondence with Dan Lindsley are archived at *F Cambridge and the source of the mistake might be apparent from examining *F those notes. Regardless, the gene entries for uncl and stnA should be *F modified. # *U FBrf0104933 *a Cowley *b G. *t 1998.9.24 *T personal communication to FlyBase *u *F From: cowley@helix.mgh.harvard.edu (Glenn Cowley) *F Subject: FlyBase Help Mail *F To: flybase-help@morgan.harvard.edu *F cowley@helix.mgh.harvard.edu (Glenn Cowley) sent the following comments to flybase-help: *F ------------------------------------------------------------ *F I have obtained plasmid rescue data from B-#P142 w1118 P{w+mC=EP}EP317. *F This insertion is within the first intron of the Ariadne gene (ari) located *F at 1-57.6-58.7 *F ------------------------------------------------------------ *F Server protocol: HTTP/1.0 Remote host: csc-6218h.mgh.harvard.edu *F Remote IP address: 132.183.130.33 # *U FBrf0104934 *a Bloomington Drosophila Stock Center *b ?. *t 1998.9.24 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: CyO and TM2 variants *F Date: 25 September1998 *F *F Information communicated: *F *F From the Gehring lab: *F CyO, P{lArB}A4.1M2 *F CyO, P{lArB}A32.1M2 *F CyO, P{lArB}A66.2F2 *F CyO, P{lArB}A79.1M2 *F CyO, P{lArB}A176.1M2 *F CyO, P{lArB}A203.3F2 *F CyO, P{lArB}A336.1F2 *F CyO, P{lArB}A350.1M2 *F CyO, P{lArB}A480.1M2 *F CyO, P{lArB}A495.1M2 *F CyO, P{lArB}B39.1M2 *F CyO, P{lArB}A109.1F2 *F CyO, P{lArB}A507.2M2 *F CyO, P{lArB}Fas3[A183.1F2] *F *F From the Jan lab: *F TM2, P{lacW}l(3)E7-3-58[1] # *U FBrf0104935 *a Ashburner *b M. *t 1998.9.30 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Wed Sep 30 11:28:01 1998 *F To: ag24@gen.cam.ac.uk *F Subject: new genes *F \*a pcdr *F > *F \*a Pdh *F these are the same *F M # *U FBrf0104938 *a Baumgartner *b S. *t 1998.10.5 *T personal communication to FlyBase *u *F From @nomina.lu.se:Stefan.Baumgartner@medkem.lu.se Fri Oct 02 17:10:59 1998 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Hem *F Dear Rachel, *F Allele l(3) 319.4 does no longer exist in our collection (the stock *F died), nor have we sent it to anybody else. So I do not know whether *F one should maintain this allele in Flybase. *F Thanks for the up-date *F Stefan *F \-- *F Stefan Baumgartner *F Lund University *F Dept. of Cell & Molecular Biology *F Section for Developmental Biology *F Box 94 *F S-22100 Lund *F SWEDEN *F Tel. Office 46 46 222 3893 *F Tel. Lab 46 46 222 3006 *F FAX 46 46 211 3417 # *U FBrf0104939 *a Okabe *b M. *t 1998.10.3 *T personal communication to FlyBase *u *F From maokabe@lab.nig.ac.jp Sat Oct 03 14:35:12 1998 *F To: flybase-help@morgan.harvard.edu *F Subject: comments about argos gene and pointed gene *F Dear Sirs, *F There appears to have been some errors made in informations about argos and *F pointed genes in flybase. *F argos: *F Our argos allele, argos257, is amorphic allele, not hypomorph, as published in Okabe et al.,1996. Recently, we also checked the argos locus in argos *F 257 by PCR and found it was deleted almost entire of the first exon including the translation initiation site. *F pointed: *F We used the allele name, pnt7848Delta78, in Okabe and Okano, 1997. However *F , It was our miss in this allele name. This allele is same as pnt7825Delta *F 78, so please change this in the information of flybase. *F Thank you. *F Masataka Okabe # *U FBrf0104941 *a Bedian *b V. *t 1998.10.2 *T personal communication to FlyBase *u *F Archived # *U FBrf0104943 *a Megraw *b T. *c T.C. *d Kaufman *t 1998.10.2 *T personal communication to FlyBase *u *F Archived # *U FBrf0104945 *a Schaeffer *b S.W. *t 1998.11.23 *T personal communication to FlyBase *u *F Archived # *U FBrf0105348 *a Heisenberg *b M. *t 1998.11.4 *T personal communication to FlyBase *u *F From eleanor@gen.cam.ac.uk Fri Oct 30 16:20:32 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Fri, 30 Oct 1998 16:20:32 +0000 *F To: Heisenberg@biozentrum.uni-wuerzburg.de *F Subject: Help FlyBase - mbm abstract *F Cc: eleanor@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Eleanor Whitfield (Genetics) *F Date: Fri, 30 Oct 1998 16:20:00 +0000 *F Content-Length: 1059 *F Hi Martin, *F I am curating an abstract of yours, from the Sixth European meeting on *F the Neurogenetics of Drosophila, for FlyBase: *F Albert et al, 1997, J. Neurogenetics 11: 141. *F I have a few questions I would like to ask you, your answers will *F clarify the data for loading into FlyBase. *F 1) In this abstract you discuss chromosome Df(2L)R70. *F Is this the deficiency segregant of Tp(2;Y)R70, the deficient region *F being between 21C3;21C5? *F If not, could you please describe the chromosome. *F 2) You describe a P-element insertion, P21BC. *F Is this a natural P-element or an engineered construct? Do you have *F any further information regarding insertion point? *F 3) Has the mbm mutation in line cn bw sp been previously described or *F is this new information? *F Have you written a paper since this abstract, maybe that will answer *F these questions? *F Many thanks for any help you can provide. *F On the advice of Stephan Schneuwly I have emailed you to help as I have *F neither a fax number or email address for S. Albert or T. Twardzik. *F Regards, *F Eleanor Whitfield *F FlyBase *F From heisenberg@biozentrum.uni-wuerzburg.de Wed Nov 04 11:49:17 1998 *F Envelope-to: eleanor@gen.cam.ac.uk *F Delivery-date: Wed, 4 Nov 1998 11:49:17 +0000 *F Comments: Authenticated sender is *F From: 'Martin Heisenberg' *F Organization: Dept. of Genetics, Univ. of Wuerzburg, Germ *F To: Eleanor Whitfield (Genetics) *F Date: Wed, 4 Nov 1998 12:50:39 +0001 *F MIME-Version: 1.0 *F Content-transfer-encoding: 7BIT *F Subject: Re: Help FlyBase - mbm abstract *F Priority: normal *F X-mailer: Pegasus Mail for Win32 (v2.52) *F Dear Eleanor: *F Thanks for your inquiry. I will go through your questions one by one. *F 1) Yes. This is the deficiency. We received it from Pasqual Heitzler, *F Stassbourg. *F 2)The P-element( PlacW 319/11) is from a screen conducted in *F collaboration with Ernst Hafen in Zurich. It contains a lacZ and a *F mini-white gene. It is inserted just to the right of a Hin dIII *F site in a 1.3kb H/E fragment in the genomic region of the phage BPal *F 14. This fragment contains an alternatively spliced exon of the plc21 *F gene. The insertion site is just to the left of the exon. All this is *F in a Doctoral Thesis of Susanne Albert which, however, is in German. *F 3) This is new and yet unpublished information. *F Part of the masses of data is in a paper by Albert et al. (1997) *F Isolation and characterization of the droPIK57 gene encoding a new *F regulatory subunit of phosphatidylinositol 3-kinase from D.m.. GENE *F 198, 181-189. (See also: Weinkove et al. (1997) p60 is an adaptor for *F the D. phosphoinositide 3-kinase, Dp110. J.Biol. Chem. 272, 14606-10). *F If you need any further information, do not hesitate to contact me *F again. *F All the best, Martin Heisenberg *F \------------ *F Prof. Dr. Martin Heisenberg *F Theodor-Boveri-Institut fuer Biowissenschaften *F Lehrstuhl fuer Genetik *F Am Hubland *F 97074 W u e r z b u r g *F Tel.: (49)-(0)931- 8884450 *F Fax.: (49)-(0)931- 8884452 *F email: heisenberg@biozentrum.uni-wuerzburg.de # *U FBrf0105373 *a Bloomington Drosophila Stock Center *b ?. *t 1998.11.2 *T personal communication to FlyBase *u *F Personal communication from: Kathy Matthews, Indiana University *F Subject: Cu[3] is a viable allele *F Date: 2 November 1998 *F *F Information communicated: *F Lindsley & Zimm states that Cu[3] (synonym Cd[2]) is a lethal allele. The reference cited, however, contains no information about Cu[3]. A stock carrying Cu[3] is in the Bloomington collection, to us from Ted Wright as Cd[2], and Cu[3] flies are homozygous viable. It seems likely that the lethal phenotype Lindsley & Zimm assigned to Cu[3] was a simple error in the allele table. *F # *U FBrf0105382 *a Pignoni *b F. *t 1998.11.9 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Oct 30 09:30:30 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 30 Oct 1998 09:30:30 +0000 *F To: francesca_pignoni@meei.harvard.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 30 Oct 1998 09:31:28 +0000 *F Content-Length: 1669 *F Dear Dr. Pignoni, *F I am curating a paper for FlyBase: *F Huang and Kunes, 1998, Development 125(19): 3753--3764 *F in which they used a 'flp-out' GAL4 line that was referenced to your paper: *F Pignoni and Zipursky, 1997, Development 124(2): 271--278 *F I wrote to Larry Zipursky with some questions about the line and he *F suggested I write to you as you made the construct. *F the line Huang and Kunes used in the paper was called 'PTub>CD2>GAL4'. *F FlyBase does not have a record of this construct - we have a similar *F construct where the promoter is Actin 5C - PActin>CD2>GAL4 (from your *F 1997 Development paper), but not tubulin. *F I would be grateful if you could tell me some details about the *F construct, so that I can include the details in a personal *F communication from you to FlyBase. *F 1. which particular tubulin promoter is used (i.e alpha1, beta2 etc.) *F 2. is the FRT cassette containing CD2 the same as used in the *F PActin>CD2>GAL4 construct from Pignoni and Zipursky, 1997, *F Development 124(2): 271--278 ? *F 3. what vector is the construct in, e.g. CaSpeR, Carnegie20 (so I can *F work out which marker gene is in the construct) ? *F 4. who made the construct (this helps in naming the construct in *F FlyBase) - I guess this was you? *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Francesca_Pignoni@meei.harvard.edu Mon Nov 09 19:15:52 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 9 Nov 1998 19:15:52 +0000 *F From: Francesca Pignoni *F To: ''Gillian Millburn'' *F Subject: RE: FlyBase query *F Date: Mon, 9 Nov 1998 13:47:25 -0500 *F X-Mailer: Internet Mail Service (5.0.1460.8) *F Content-Length: 602 *F Dear Gillian *F > 1. which particular tubulin promoter is used (i.e alpha1, beta2 etc.) *F Tubulin alpha 1 *F > 2. is the FRT cassette containing CD2 the same as used in the *F > PActin>CD2>GAL4 construct from Pignoni and Zipursky, 1997, *F > Development 124(2): 271--278 ? *F yes, identical *F > *F > 3. what vector is the construct in, e.g. CaSpeR, Carnegie20 (so I can *F > work out which marker gene is in the construct) ? *F CaSpeR 2 , mini-white *F > *F > 4. who made the construct (this helps in naming the construct in *F > FlyBase) - I guess this was you? *F Yes, I did *F Francesca # *U FBrf0105410 *a Gelbart *b W.M. *t 1998.10.20 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Tue Oct 20 18:16:51 1998 *F To: ag24@gen.cam.ac.uk *F Subject: Re: Tp(2;2)DTD48 report *F Cc: flybase-updates@morgan.harvard.edu *F Valid aberration name: Tp(2;2)DTD48 *F Synonym: 434.84 *F Induced upon: dppd-ho bearing second chromosome. dppd-ho is *F contained within transposed segment. *F Break 1: 22E2-4 *F Break 2: 22F4-23A1 *F Break 3: 53D1-2 *F New order: 2Lt - 22E | 23A - 53D | 22F - 22E | 53D - 2Rt. *F Comments: Deficiency segregant is haplo-lethal except in presence of *F duplication of functional copy of dpp-Hin region. Deficiency *F lacks dpp and oaf but is wild-type for l(2)22Fc and l(2)22Fd. *F Deficiency also lacks several vital loci distal to dpp. *F Duplication segregant wild-type for all dpp functions *F except dppd-ho. Duplication is wild-type for oaf and *F for several vital loci distal to dpp. The duplication *F covers all vital loci distal to dpp that are exposed *F by Df(2L)DTD2. # *U FBrf0105411 *a Bloomington Drosophila Stock Center *b ?. *t 1998.10.20 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: bnch[Delta58], fs(3)85Aa[e5] and H{PDelta2-3}HoP8 *F Date: 21 October 1998 *F *F Background: Alleles and a transposon insertion at Bloomington but not in FlyBase. *F *F Information communicated: *F *F bnch[Delta58] *F To us from Hugo Bellen. This is a null allele created by excision of the P-element reported in Kania et al., Genetics, 1995. *F *F fs(3)85Aa[e5] *F To us from Thom Kaufman, EMS-induced. *F *F H{PDelta2-3}HoP8 *F To us from Bill Gelbart, insertion on the X. # *U FBrf0105412 *a Sanchez-Herrero *b E. *t 1998.8.30 *T personal communication to FlyBase *u *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Ernesto Sanchez-Herrero *F To: Mid-America Drosophila Stock Center *F Subject: Lethals in 89 *F Date: 30 August 1989 *F *F Background: These lethals were obtained in the screen referred to in Sanchez-Herrero et al., Nature 313, 108-113 (1985), but are otherwise unpublished. They were induced with EMS on a mwh[1] jv[1] st[1] red[1] Sb[sbd-2] e[11] ro[1] ca[1] chromosome. A subset of these lethals are available in stock from the Bloomington or Umea stock centers. The gene *F synonyms included below are those in use by the Mid-America stock list when stocks were transferred to Bloomington. Complementation data were derived from a map dated 30 Aug 1989, personal communication from Ernesto Sanchez-Herrero to the Mid-America Drosophila *F Stock Center, received by Bloomington from Mid-America on 8 Dec 1997. New l(3)89-style gene names were assigned by the Bloomington stock center based on names available as of 21 Oct 1998. *F *F Information communicated: *F *F New genes and alleles: *F l(3)89Bu[M1511] *F gene synonym l(3)M1511 *F Fails to complement Df(3R)P115 and Df(3R)bxd100. *F Complements Df(3R)P10, Df(3R)Ubx109, Df(3R)P9 and Df(3R)C4. *F Is not included within Dp(3;3)S462. *F *F l(3)89Cg[M191] *F gene synonym l(3)M191 *F Fails to complement Df(3R)P115, Df(3R)bxd100 and Df(3R)P10. *F Complements Df(3R)Ubx109, Df(3R)P9 and Df(3R)C4. *F Is not included within Dp(3;3)S462. *F *F l(3)89Ch[M2912] *F l(3)89Ch[M291] *F gene synonym l(3)M2912 *F Fails to complement Df(3R)P115, Df(3R)bxd100 and Df(3R)P10. *F Complements Df(3R)Ubx109, Df(3R)P9 and Df(3R)C4. *F Is not included within Dp(3;3)S462. *F *F l(3)89Ci[M238] *F gene synonym l(3)M238 *F Fails to complement Df(3R)P115, Df(3R)bxd100 and Df(3R)P10. *F Complements Df(3R)Ubx109, Df(3R)P9 and Df(3R)C4. *F Is not included within Dp(3;3)S462. *F *F l(3)89Dd[M175] *F gene synonym l(3)M175 *F Fails to complement Df(3R)P115, Df(3R)bxd100 and Df(3R)P10. *F Complements Df(3R)Ubx109, Df(3R)P9 and Df(3R)C4. *F Included within Dp(3;3)S462. *F *F l(3)89De[M188] *F l(3)89De[M288] *F gene synonym l(3)M188 *F Fails to complement Df(3R)P115, Df(3R)bxd100, Df(3R)P10 and Df(3R)Ubx109. *F Complements Df(3R)P9 and Df(3R)C4. *F Included within Dp(3;3)S462. *F *F l(3)89Df[M2922] *F gene synonym l(3)M2922 *F Fails to complement Df(3R)P115, Df(3R)bxd100, Df(3R)P10 and Df(3R)Ubx109. *F Complements Df(3R)P9 and Df(3R)C4. *F Included within Dp(3;3)S462. *F *F l(3)89Em[M96] *F l(3)89Em[M2914] *F gene synonym l(3)M96 *F Fails to complement Df(3R)P115 and Df(3R)C4. *F Complements Df(3R)bxd100, Df(3R)P10, Df(3R)Ubx109 and Df(3R)P9. *F Included within Dp(3;3)S462. *F *F l(3)89En[M2511] *F gene synonym l(3)M2511 *F Fails to complement Df(3R)P115 and Df(3R)C4. *F Complements Df(3R)bxd100, Df(3R)P10, Df(3R)Ubx109 and Df(3R)P9. *F Included within Dp(3;3)S462. *F *F New alleles of genes already known to FlyBase: *F l(3)89Bm[M231] *F l(3)89Bm[M268] *F l(3)89Bm[M2918] *F l(3)89Bn[M154]> *F l(3)89Bn[M52] *F l(3)89Ce[ME21] *F l(3)89Cf[M203] *F l(3)89Cf[M233] *F gene synonym l(3)M203 *F l(3)89Ea[M182] *F l(3)89Ea[M251] *F gene synonym l(3)M182 *F l(3)89Ek[M163] *F l(3)89El[M171] *F l(3)89El[M297] *F gene synonym l(3)M171 # *U FBrf0105413 *a Matthews *b K. *t 1998.11.5 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: l(3)j1E4[j1E4] is semi-lethal *F Date: 5 November 1998 *F *F Information communicated: *F *F l(3)j1E4[j1E4] is a semi-lethal allele. *F # *U FBrf0105415 *a Bernards *b A. *t 1998.11.6 *T personal communication to FlyBase *u *F From abernard@helix.mgh.harvard.edu Fri Nov 06 16:36:49 1998 *F Subject: Flybase entry on NF1 *F To: *F Dear Sir: *F There are a few inaccuracies in the flybase entry on the Nf1 gene. The *F entry says that Nf1 is located at 96F11 and the map of the region places *F the gene telomeric to the E(spl) complex. In reality the Nf1 gene maps *F between the bride-of-sevenless and E(spl) loci at 96F (The et al., *F Science 276:791-794; 1997). The gene is transcribed from centromer to *F telomer (boss - 5' Nf1 3' - E(spl). Rather than mouse Nf1, the most *F obvious homolog of Nf1 is the human neurofibromatosis type 1 tumor *F suppressor gene. Biochemically, the human and fly NF1 proteins are GTPase *F activating proteins for Ras. However, Nf1 deficient phenotypes in *F Drosophila (reduced size, absence of a PACAP-induced rectifying potassium *F current at the larval neuromuscular junction) are not modified by *F manipulating Ras1 or Ras2 gene dosage, but rather are rescued by *F increasing signaling through the cAMP - PKA pathway. Thus, Nf1 functions *F either upstream of adenylyl cyclase or in a parallel pathway. Saying that *F Nf1 is a crucial component for activation of the cAMP pathway is *F overstating the case. *F I hope this was helpful. Please feel free to contact me if further *F information would be required. *F Best regards, *F Andre Bernards *F Andre Bernards Ph.D. *F Associate Professor of Medicine (Genetics) *F Massachusetts General Hospital Cancer Center *F Bldg. 149, 13th street *F Charlestown, MA 02129 *F Tel: (617) 726-5620 *F Fax: (617) 724-9648 # *U FBrf0105416 *a Roote *b J. *t 1998.11.16 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Nov 16 14:20:28 1998 *F To: rd120@mole.bio.cam.ac.uk *F Subject: new P alleles *F Rachel, *F I don't know which of these you already have a note of.. but anyway here is *F a list of P elements which have been mapped recently both genetically and *F by Berkeley sequence to genes in the Adh region: *F k02602 CycE 35D3-4 lethal over CycEk00807 *F k03505 crp 35F11-12 viable with r10 but *F narrow, pointed wings *F k07233 vasa 35C1-3 reduced female fertility over vas- *F deletions *F k07904 Su(H) 35B8-9 lethal over Su(H)1356 *F k08310 twe 35F1-2 male sterile *F k10011 l(2)35Bg 35B10-11 semi-lethal over *F 35BgOK5 *F k14422 35Df 35D5-7 cluster-mate of k14423 *F k16716 wb 35A1-2 wingblister over wbSF25 *F EP0613 twe 35F1-2 male ster,female fert over twe- *F deletion *F EP2039 elB 35B1-2 v.wk el over A217 *F EP2159 esg 35D1-2 lethal over TE35D-12 *F rJ571 osp osp- over osp76e *F EP0812 vasa 35B10-C1 female sterile over vas- deletion *F Also: *F Df(2L)k15711 35C1-3 deletion 35Bb-esg+/- *F female sterile esg allele. *F k04206 36A1-2 lethal with H20 *F John # *U FBrf0105417 *a Stiffler *b L. *t 1998.11.16 *T personal communication to FlyBase *u *F From lstiff@u.washington.edu Mon Nov 16 20:02:50 1998 *F To: Fly Bloomington *F Subject: neo114 is solved *F Dear Stock Center- *F I emailed you guys awhile ago regarding the origins of the neo114 line. *F Here is the response that I received from Spradling if you want to update *F your information. *F \---------- Forwarded message ---------- *F Date: 16 Nov 1998 14:02:01 -0500 *F From: Allan Spradling *F To: Lisa Stiffler *F Subject: Re: neo 114 stock *F Reply to: RE>neo 114 stock *F Dear Lisa: *F Yes, these neo 114 and neo 17 are different names for the same stock isolated *F in Lynn Cooley's 1987 single P element screen, and used by Edgar and O'Farrell *F to define the first CycA mutations. We changed the original strain numbers *F to consecutive numbers when the work was published in Science, but we had *F already distributed the lines under the old names to quite a few labs. When *F we sent the lines to Bloomington, we included the information on the synonyms, *F and it was listed for a while in old Hypercard versions of their P element *F stock list. With the advent of Flybase, this information was transfered to the *F synonym list, and I find that neo114 is currently listed as a synonym for *F CycA. Unfortunately, the entry does not make it clear enough that neo114 and *F neo17 are identical. *F Allan Spradling *F Dear Dr. Spradling, *F I have been trying to track down the source for the line neo114. I *F received the stock from Bruce Edgar, who I believe got it from Pat *F O'Farrell. I have been trying to determine if it is the same stock as the *F neo17 line published in Science some time ago. *F . *F . *F . *F Thank you for *F your time. *F Sincerely, *F Lisa Stiffler (Schubiger lab) *F \----- End Included Message ----- # *U FBrf0105422 *a Tavosanis *b G. *t 1998.11.24 *T personal communication to FlyBase *u *F From Gaia.Tavosanis@embl-heidelberg.de Tue Nov 24 12:40:41 1998 *F To: Rachel Drysdale (Genetics) *F Subject: Re: fs(2)TW1.1 *F Dear Rachel, *F thank you for your mail. The information regarding &ggr;Tub37C1 is the *F following: *F we identified a base pair substitution (G to A) in position 1027 of the *F CDS, from the ATG. This produces an amino acid substitution from W to STOP *F in position 352. The truncated protein lacks the last 105 C-terminal *F amino acids, and we think it is very likely to be rapidly degraded. *F Thank you in advance, *F regards, *F Gaia Tavosanis *F > Dear Gaia, *F > *F > Thank you for your mail about &ggr;Tub37C1 (&ggr;Tub37CDTW1.1). We *F > have the accession number AF081253 recorded for that allele but from *F > simply viewing the Genbank record it is not clear what the mutant *F > lesion is, with respect to the wild type sequence. For example is there *F > a base pair substitution leading to an amino acid substitution? If you *F > can summarise this for me in a few words I can incorporate this into *F > the allele record, citing your mail as a personal communication *F > including the data. *F > *F > Apologies for the delay in our reply to you - FlyBase have been at a *F > meeting this week. Normal service has now been resumed. *F > *F > Regards, *F > *F > Rachel. *F Gaia Tavosanis *F EMBL *F Cell Biology Programme *F Meyerhofstr. 1 *F Postfach 10 22 09 *F 69117 Heidelberg *F Tel. +49 6221 387 293 *F Fax: +49 6221 387 306 *F Tavosanis@embl-heidelberg.de # *U FBrf0105424 *a Matunis *b E. *t 1998.11.24 *T personal communication to FlyBase *u *F >Envelope-to: jr32@mole.bio.cam.ac.uk *F >Date: 24 Nov 1998 10:09:32 -0500 *F >From: 'Erika Matunis' *F >Subject: Re: ms(2)916 *F >To: 'John Roote' *F > *F > RE>ms(2)916 11/24/98 *F > *F >Dear John, *F > *F >Although it was found in a p-mutagenesis, it is *F >not tagged by the p and so therefore has not been mapped cytologically. Only *F >meiotically, which is much less reliable, especially since ms(2)916 is not *F >even a male sterile, so that all the experiments with this allele rely on *F >searching for the partially penetrant phenotype in dissected testes. And it *F >is not uncovered by any of the Df's in the chromosome II Df kit. *F > *F >-------------------------------------- # *U FBrf0105425 *a Bloomington Drosophila Stock Center *b ?. *t 1998.11.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Nov 24 16:59:50 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 24 Nov 1998 16:59:50 +0000 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: Windows Eudora Light Version 3.0.1 (32) *F Date: Tue, 24 Nov 1998 11:59:25 -0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: CyO, cl4 *F Mime-Version: 1.0 *F Hi Rachel-- *F Yet another thing: *F A new balancer variant CyO, cl4 in stock 4902 Gpon322/CyO, cl4 from *F Ross MacIntyre. *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 812-855-5782 *F Indiana University *F Bloomington, IN 47405-6801 # *U FBrf0105492 *a Lopez *b J. *t 1998.11.24 *T personal communication to FlyBase *u *F Date: Tue, 19 Jun 2001 12:12:30 -0500 (EST) *F From: Kathy Matthews *F Subject: Embryonic Expression Patterns of GAL4 Enhancer Trap Lines *F To: flybase-updates@morgan.harvard.edu *F *F Personal communication from: Javier Lopez, Carnegie-Mellon University *F To: Bloomington Drosophila Stock Center *F Subject: Embryonic Expression Patterns of GAL4 Enhancer Trap Lines *F Dated: 1 September 1995 *F *F Background: This is a scanned version of hardcopy provided to the stock *F center by Javier and to FlyBase by the stock center. That hardcopy was *F archived at FlyBase as FBrf0105492. This file is intended to replace *F the hardcopy version at FlyBase. The stock center will keep the original *F hardcopy on file. *F *F In all cases the transgene construct is P{GawB}. Cl-76D in the following *F list is also known as I-76-D. *F *F Information communicated: *F *F APPENDIX II *F *F SUMMARY OF EMBRYONIC EXPRESSION PATTERNS OF P[GAL4] ENHANCER TRAP LINES *F *F LINE \# TISSUES SHOWING EXPRESSION *F *F V1 SALIVARY GLANDS (SG) *F V2 SG *F V4 CNS, PNS *F V7A SG *F V8 SG *F V9B SG *F V11A CNS *F V12 SG *F V14 SG *F V16 SG *F V17 PNS *F V19 SG *F V24 SG *F V28 SG *F V30A SG *F V32A SG *F V34 EPIDERMIS, STRONG LATE EXPRESSION *F V39 SG *F V43 SG *F V44 SG *F V45 SG *F V46 SG *F V49 EPIDERMIS *F V51 PNS *F V54 SG *F V55 PNS *F V58 -- *F V59 MIDGUT, MESECTODERM *F V61 MIDGUT *F V65 -- *F V66 SG *F V67 SG *F V68 SG *F V70 MIDGUT *F V72 MIDGUT *F V73 SG *F V74 SG *F V76 SG *F V77 VISCERAL MESODERM OR ENDODERM *F V78 -- *F V82 LYMPH GLAND, MIDGUT, FATBODY *F V83 SG *F V84 SG *F V85 ALL NEURONS *F V86 LYMPH GLAND, MIDGUT, FATBODY *F V89 SG *F V90 SG *F V90B SG *F V91 SG *F V92 SG *F V94 SG *F V95 HINDGUT *F V98 CNS, PNS *F V100 MIDGUT *F V101 PHARYNX *F V103 SG *F V104 SG *F V107 SG *F V109 CNS, MESODERM (SEGMENTAL PATTERN) *F V110 SG *F V111 SG *F V113 SG *F V114 SG *F V115 SG *F V121A PNS *F V121B FATBODY, MIDGUT, LYMPH GLAND *F V124 SG *F V125 SG *F V126 SG *F V127 SG *F V1211 PNS, MUSCLE *F V1253 ' *F V1132 ' *F 5001 FAINT STAINING IN PS7 *F 5004 SG, SCATTERED CELLS IN THE EPIDERMIS *F 5007 SG *F 5011B SG *F 5012 SG, SCATTERED EPIDERMAL CELLS *F 5015 LYMPH GLAND *F 5016 SG *F 5018 SG *F 5020A SG *F 5023 SG *F 5024 SG *F 5025 SG, SOME EPIDERMAL CELLS *F 5026 SG, SOME EPIDERMAL CELLS *F 5028 PNS *F 5029 SG *F 5031 B PNS *F 5036 SG, SOME EPIDERMAL CELLS *F 5038 SG *F 5039 SG *F 5040A SG, SOME EPIDERMAL CELLS *F 5041 SG *F 5043 SG *F 5044 SG *F 5046 LYMPH GLAND *F 5048 SG *F 5049A LONGITUDINAL MUSCLES, SOME GUT MUSCLES *F 5049B EARLY SG, LYMPH GLAND *F 5050 PHARYNX *F 5051 SG, SOME EPIDERMAL CELLS *F 5052B FAINT STAINING IN VISCERAL MESODERM *F 5053A LONGITUDINAL MUSCLES, SOME CELLS IN GUT *F 5053B LONGITUDINAL MUSCLES, SOME CELLS IN GUT *F 5056 SG *F 5057 SG, SOME EPIDERMAL CELLS *F 5058 SG, SOME EPIDERMAL CELLS *F 5059 EARLY SG, SOME STAINING IN MIDGUT *F 5060 STRONG STAINING IN EPIDERMIS, HINDGUT *F 5061 STRONG STAINING IN EPIDERMIS, HINDGUT *F 5062 FAINT STAINING IN TRANSVERSE MUSCLES *F 5064 SG *F 5065 SG, LYMPH GLAND *F 5066 SG *F 5067 PATCH OF DORSAL MESODERM *F 5069 SPOTTY STAINING IN THE MIDGUT *F 5070 SG *F 5074 PNS OR MUSCLE *F 5075 FAINT STAINING IN MESODERM (NOT CONSISTENT) *F 5077 SG *F 5078 SG, LYMPH GLAND *F 5082 SG, LYMPH GLAND *F 5088 SG, LYMPH GLAND *F 5090 VERY EARLY SG, LYMPH GLAND *F 5093 LYMPH GLAND, VISCERAL MESODERM, ENDODERM *F 5094 -- *F 5095 PHARYNX *F 5096 PHARYNX *F 5097 MIDGUT *F 5099 SG *F 5103 MIDGUT IN LATE EMBRYOS *F 5104 SG *F 5105 EPIDERMIS (SEGMENTAL PATTERN), CNS, PNS *F 5108 LYMPH GLAND, ANTERIOR DORSAL VESSEL *F C13 PHARYNX *F C15 ENDODERM *F C18 SG *F C23 TRANSVERSE MUSCLES *F C25 SG *F C27 SG *F C35 LYMPH GLAND *F C36 PNS *F C37 LYMPH GLAND *F Cl-41 PART OF GUT, ANAL PLATES *F Cd-41 SG *F C43 AMNIOSEROSA *F C51 PNS *F C54 SG *F C58 SG *F C59 SG *F C65 SCATTERED CELLS THROUGHOUT THE EMBRYO *F C67 SG *F C72 -- *F Cl-76D EPIDERMIS *F C77 DORSAL VESSEL OR DORSAL CLOSURE *F C78 YOLK *F C83 SG *F C84 TRANSVERSE MUSCLES, PROVENTRICULUS *F C86 SOME CELLS IN THE LATERAL CNS *F C87 PHARYNX *F C89 SG *F C90 -- *F C91 SG *F C93 SG *F C96 ANTERIOR GUT, ATRIUM *F C97 SG *F C1003 CNS, EPIDERMIS, PNS *F Cl-1006 PNS *F C1007 SG, SCATTERED CELLS IN EPIDERMIS *F Cd-1008 PNS *F C1009 SG *F C1010 SG *F C1011 SG *F C1019 SG *F C1021 -- *F C1023 SG *F C1025 SG *F C1026 SG *F C1027 SG # *U FBrf0105493 *a Huet *b F. *c M. *d Crosby *e R. *f Baugh *t 1999.1.7 *T personal communication to FlyBase *u *F From crosby@morgan.harvard.edu Thu Jan 07 19:57:33 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: pc regarding yellow constructs *F To: FlyBase *F From: Francois Huet, Madeline Crosby, Ryan Baugh *F We wish to report as a personal communication the following *F information regarding pP{Car20y} and derived constructs. *F It has been previously reported (Nassif et al., 1994, MCB 14:1613) *F that the SalI y+ segment derived from pP{Car20y} is not 7.7kb as *F originally claimed, but approximately 7.9kb. It was established *F by Nassif et al. that the extra ~200bp are at the 3' end of the *F yellow segment. *F Using several constructs derived from pP{Car20y}, we have confirmed *F these results and have sequenced the unidentified fragment. The *F sequence is presented below, starting with the BglII site known to be *F at the 3'end of yellow, and ending with the SalI site used to clone *F into and out of pP{Car20y}. 19bp at the 3'end appear to be derived *F from a polylinker; the first 157bp are identical to sequence located *F 1.7kb downstream from the yellow fragment in cosmid clone DMC125H10 *F (accession number AL023873; 21264-21420). Ligation of the yellow *F fragment with the 157bp fragment appears to have occurred at the *F BglII site at the 3' end of yellow and the BglII site at the 5' end *F of the 157bp fragment. A new BamHI site has been created at 163-168. *F Sequence: *F agatcttgtt tgggtgcagg gaaagttcaa cttaatcgct caatttgaga tcgcctggtc *F gcttgagatt cgactgtaat tgaaattttt gcttttgatc ggagccagac ttcagacggg *F gcaaacaaaa agactttgtt ggtggtaggg taggatccgt tgacctgcag gtcgac *F This extra fragment may also be present in mini-yellow and other *F constructs derived from pP{Car20y}. *F From rd120@gen.cam.ac.uk Fri Jan 08 13:55:17 1999 *F To: crosby@morgan.harvard.edu *F Subject: Re: pc regarding yellow constructs *F Hi Lynn, *F It seems sensible to rename the allele y+t7.9. Do you suppose it *F also corresponds to y+t8? *F From crosby@morgan.harvard.edu Fri Jan 08 15:31:50 1999 *F To: rd120@gen.cam.ac.uk *F Subject: Re: pc regarding yellow constructs *F Rachel, *F >It seems sensible to rename the allele y+t7.9. Do you suppose it *F >also corresponds to y+t8? *F I believe they are all the same thing (this finding nicely explains the *F discrepancies). HOWEVER, y+t7.7 is the more correct symbol -- the extra *F 170bp may not be related to yellow. I think it must be due to some sort *F of cloning glitch; it is an apparently random fragment about 1.7kb away, *F probably present in the original genomic clone. *F y+t7.9 and y+8 are truly incorrect -- since the sequence order is not *F as found in the wild type. *F \--Lynn # *U FBrf0105494 *a Geyer *b P. *t 1999.1.9 *T personal communication to FlyBase *u *F From pamela-geyer@uiowa.edu Sat Jan 9 11:58:55 1999 *F To: Madeline Crosby *F Subject: Re: yellow constructs -- extra fragment *F Dear Madeline, *F Thanks for you message. Yes, I knew about this. The way the original *F construct was made, an approximately 2.0 kb Bgl II fragment in the 3' end *F of the yellow gene was removed. Thus, these sequences are all sequences of *F the region of yellow, there is just a discontinuity in these clones *F relative to the genomic DNA. When I distribute plasmids to anyone, I tell *F them of this change. However, people get the plasmids from other sources, *F and not all of the original information is passed on. *F Thus, I would not call this DNA an extra fragment, since it is still from *F the yellow region. *F I take it from your comments, you will enter a complete sequence of the *F yellow clones in Flybase??? *F Thanks for your information! *F Pam *F > From crosby@morgan.harvard.edu Thu Jan 7 15:15:51 1999 *F > To: pamela-geyer@uiowa.edu *F > Subject: yellow constructs -- extra fragment *F > *F > *F > Pam, *F > *F > Thought we should relay this to you. This extra fragment appears *F > to be in both pBSX and in yS/GpUC8 (obtained via Ting Wu). You *F > may know all about it already, but I wanted to get it officially *F > into FlyBase, in order to correct some compiled sequences. *F > *F > *F > --Lynn *F > *F > *F > ----- Begin Included Message ----- *F > *F > From crosby@morgan.harvard.edu Thu Jan 7 14:59:49 1999 *F > To: flybase-updates@morgan.harvard.edu *F > Subject: pc regarding yellow constructs *F > Cc: huet@morgan.harvard.edu, crosby@morgan.harvard.edu *F > X-Sun-Charset: US-ASCII *F > Content-Length: 1530 *F > *F > *F > *F > To: FlyBase *F > *F > From: Francois Huet, Madeline Crosby, Ryan Baugh *F > *F > *F > We wish to report as a personal communication the following *F > information regarding pP{Car20y} and derived constructs. *F > *F > It has been previously reported (Nassif et al., 1994, MCB 14:1613) *F > that the SalI y+ segment derived from pP{Car20y} is not 7.7kb as *F > originally claimed, but approximately 7.9kb. It was established *F > by Nassif et al. that the extra ~200bp are at the 3' end of the *F > yellow segment. *F > *F > Using several constructs derived from pP{Car20y}, we have confirmed *F > these results and have sequenced the unidentified fragment. The *F > sequence is presented below, starting with the BglII site known to be *F > at the 3'end of yellow, and ending with the SalI site used to clone *F > into and out of pP{Car20y}. 19bp at the 3'end appear to be derived *F > from a polylinker; the first 157bp are identical to sequence located *F > 1.7kb downstream from the yellow fragment in cosmid clone DMC125H10 *F > (accession number AL023873; 21264-21420). Ligation of the yellow *F > fragment with the 157bp fragment appears to have occurred at the *F > BglII site at the 3' end of yellow and the BglII site at the 5' end *F > of the 157bp fragment. A new BamHI site has been created at 163-168. *F > *F > Sequence: *F > *F > agatcttgtt tgggtgcagg gaaagttcaa cttaatcgct caatttgaga tcgcctggtc *F > gcttgagatt cgactgtaat tgaaattttt gcttttgatc ggagccagac ttcagacggg *F > gcaaacaaaa agactttgtt ggtggtaggg taggatccgt tgacctgcag gtcgac *F > *F > *F > This extra fragment may also be present in mini-yellow and other *F > constructs derived from pP{Car20y}. # *U FBrf0106031 *a Merdes *b G. *t 1998.12.20 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Dec 11 10:50:21 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 11 Dec 1998 10:50:21 +0000 *F To: g.merdes@mail.zmbh.uni-heidelberg.de *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 11 Dec 1998 10:51:47 +0000 *F Content-Length: 5167 *F Dear Gunter, *F in October you wrote to FlyBase, pointing out that the FlyBase gene *F with the ID number FBgn0008635 codes for beta-COP (we had it wrongly as *F beta-prime-COP) and that you had cloned the 'real' beta-prime-COP. *F Here is a copy of the e-mail you sent: *F > From g.merdes@mail.zmbh.uni-heidelberg.de Thu Oct 15 14:21:40 1998 *F > Envelope-to: gm119@gen.cam.ac.uk *F > Delivery-date: Thu, 15 Oct 1998 14:21:40 +0100 *F > From: 'Gunter Merdes' *F > To: flybase-updates@morgan.harvard.edu *F > Subject: FBgn0008635 *F > Date: Thu, 15 Oct 1998 15:24:32 +0200 *F > Content-Length: 1067 *F > *F > Dear colleagues, *F > *F > The gene 'beta-prime coatomer protein' with the FlyBase ID number FBgn0008635 *F > indeed codes for 'beta-COP' according to its homology to beta-COP proteins from *F > a variety of species and according to the orig. paper from Ripoche et al. 1994. *F > It should therefore be renamed. *F > *F > I have recently cloned the cDNAs for the 'real' Drosophila beta-prime subunit *F > and the alpha COP subunit: *F > *F > beta-prime COP: *F > *F > gene: Drosophila m. coatomer beta-prime subunit; beta'-COP; beta-prime COP *F > cytological localization: 34B5-34B9 *F > EMBL-Acc-No: AJ006523 (cDNA) *F > AJ006524 (genomic) *F > *F > *F > alpha COP: *F > *F > gene: Drosophila m. mRNA for coatomer alpha subunit; alpha-COP *F > cytological localization: 62A10-62B5 *F > EMBL-Acc-No: AJ011114 *F > *F > Please feel free to contact me if you have any questions or need any additional *F > information. *F > *F > With best wishes, *F > *F > Gunter Merdes *F > *F > *F > *F > *F > *F > *F > ZMBH *F > University of Heidelberg *F > Im Neuenheimer Feld 282 *F > 69120 Heidelberg *F > Germany *F > *F > Tel: 49/6221/546872 *F > Fax: 49/6221/545893 *F > email: g.merdes@mail.zmbh.uni-heidelberg.de *F > *F > *F When I was looking at our files and your e-mail, I realised that in *F your e-mail you have given cytological information for both *F beta-prime-COP and alpha-COP that we do not have in our files and is *F not in the DNA accession records for these genes. The reason I am *F writing to you is that I would like to include this information, *F specifically: *F beta-prime COP: cytological localization: 34B5-34B9 *F alpha COP: cytological localization: 62A10-62B5 *F as a personal communication from you to FlyBase, as it is always very *F useful to have cytological information about genes. Am I correct in *F thinking that you obtained the cytology by in situ hybridisation *F (rather than deficiency mapping) - as we like to include the method by *F which the cytology was determined, if possible. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From g.merdes@mail.zmbh.uni-heidelberg.de Sun Dec 20 22:10:03 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 20 Dec 1998 22:10:03 +0000 *F From: 'Gunter Merdes' *F To: gm119@gen.cam.ac.uk *F Subject: COP *F Date: Sun, 20 Dec 1998 23:15:56 +0100 *F Content-Length: 405 *F Dear Gillian, *F the cytology was indeed obtained by in situ hybridisation and verified by *F comparison of my sequences with the already sequenced P1 clones from BDGP. *F Gunter *F Gunter Merdes *F ZMBH *F University of Heidelberg *F Im Neuenheimer Feld 282 *F 69120 Heidelberg *F Germany *F Tel: 49/6221/546872 *F Fax: 49/6221/545893 *F email: g.merdes@mail.zmbh.uni-heidelberg.de # *U FBrf0106040 *a Struhl *b G. *t 1999.1.11 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Mon Jan 11 09:48:52 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 11 Jan 1999 09:48:52 +0000 *F To: struhl@cuccfa.ccc.columbia.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 11 Jan 1999 09:50:44 +0000 *F Content-Length: 1525 *F Dear Dr. Struhl, *F I am curating a paper for FlyBase (Campbell and Tomlinson, 1998, *F Development 125(22): 4483-4493) in which a UAS-GFP construct made by *F you is used. We do not have a record for this construct in FlyBase. *F I wrote to Gerard Campbell requesting some details about this construct *F and he sent me the information you sent them about this line: *F The UAS-GFP transgenes were in fact made by me. They're just a *F GFP(S65T?)-6XMYC-NLS coding sequence stuck into the pUAST vector. You *F can refer *F to them as Struhl, unpublished if you like. *F I am writing to check that it is OK for me to include this information *F in a personal communication from you to FlyBase, so that the molecular *F details of this construct make it into FlyBase. *F I have a couple of questions about the construct: *F a. is the GFP used the S65T version ? *F b. do you know what the nuclear localisation signal (NLS) is - in the *F constructs we have with NLS's, people have used 3 different sorts: *F 1. a 6 amino acid tag: KKKRKV *F 2. part of the large T antigen of SV40 *F 3. part of the glucocorticoid receptor from rat. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From struhl@cuccfa.ccc.columbia.edu Mon Jan 11 14:31:04 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 11 Jan 1999 14:31:04 +0000 *F Date: Mon, 11 Jan 1999 09:34:31 -0500 *F From: 'Gary Struhl' *F Reply-To: 'Gary Struhl' *F To: gm119@gen.cam.ac.uk *F Subject: Re: FlyBase query *F Content-Length: 1970 *F Dear Gillian, *F In answer to your questions: *F a - it is the S65T version of GFP *F b - it is the SV40 NLS PPKKKRKVED *F The same coding sequence was used in a hs-GFP-MYC-NLS transgene published in *F Jiang and Struhl, Nature, 1998. *F Hope that helps. *F Best wishes. *F Gary *F In message Gillian Millburn (Genetics) *F writes: *F > Dear Dr. Struhl, *F > *F > I am curating a paper for FlyBase (Campbell and Tomlinson, 1998, *F > Development 125(22): 4483-4493) in which a UAS-GFP construct made by *F > you is used. We do not have a record for this construct in FlyBase. *F > *F > I wrote to Gerard Campbell requesting some details about this construct *F > and he sent me the information you sent them about this line: *F > *F > The UAS-GFP transgenes were in fact made by me. They're just a *F > GFP(S65T?)-6XMYC-NLS coding sequence stuck into the pUAST vector. You *F > can refer *F > to them as Struhl, unpublished if you like. *F > *F > I am writing to check that it is OK for me to include this information *F > in a personal communication from you to FlyBase, so that the molecular *F > details of this construct make it into FlyBase. *F > *F > I have a couple of questions about the construct: *F > *F > a. is the GFP used the S65T version ? *F > b. do you know what the nuclear localisation signal (NLS) is - in the *F > constructs we have with NLS's, people have used 3 different sorts: *F > *F > 1. a 6 amino acid tag: KKKRKV *F > *F > 2. part of the large T antigen of SV40 *F > *F > 3. part of the glucocorticoid receptor from rat. *F > *F > I look forward to hearing from you, *F > *F > Gillian *F > *F > -------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > -------------------------------------------------------------- # *U FBrf0106079 *a Bloomington Drosophila Stock Center *b ?. *t 1998.11.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Nov 24 16:40:41 1998 *F To: rd120@gen.cam.ac.uk *F Subject: A couple more things *F Hi Rachel-- *F . *F . *F We received a stock from Norbert Perrimon, *F 4694 y1 sc1 lz9 rtv8/FM7a *F that is marked with an allele of lz not in FB, called lz9. Norbert got *F the stock from Billy Geer. *F Thanks, *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 812-855-5782 *F Indiana University *F Bloomington, IN 47405-6801 # *U FBrf0106080 *a Bender *b M. *t 1998.11.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 30 22:03:17 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Bender lethals *F >Return-Path: *F >Date: Mon, Nov 30, 1998 11:59 AM *F >From: 'michael bender' *F >To: kcook@bio.indiana.edu *F >Subject: Re: Question about lethals *F > *F >Kevin, *F > *F >1 will be fine for allele designations. The 4LL1 etc are the only *F designation *F >that I have given these. *F > *F >Michael *F > *F >In message <3.0.1.32.19981124114942.00f999b4@sunflower.bio.indiana.edu> *F Kevin *F >Cook writes: *F >> Hi-- *F >> *F >> One more question about the stocks you sent us. *F >> *F >> Did you assign allele numbers to the stocks of l(2)4LL1, l(2)5CC1 and *F >> l(2)6G1 you sent us? I called them all allele 1 in our stock list, but *F >> then I realized that you had isolated multiple alleles of 6G1 and 4LL1. *F >> I'd rather use your allele names if you have some; otherwise, I'll go with *F >> 1. Your DIS article didn't give allele names, so FlyBase doesn't have *F >> allele entries for the three loci. I'll communicate any info about allele *F >> names to FlyBase. *F >> *F >> Thanks for answering yet another question! *F >> *F >> Kevin *F >> _____________________________________________________________________ *F >Michael Bender, Ph.D. email bender@bscr.uga.edu *F >Assistant Professor tel (706) 542-0529 *F >Department of Genetics fax (706) 542-3910 *F >The University of Georgia *F >Athens, GA 30602 *F > *F > # *U FBrf0106081 *a Carpenter *b A.T.C. *t 1998.11.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Nov 24 15:25:35 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Info on Adelaide Carpenter's stocks *F Hi Rachel-- *F We recently received a batch of stocks from Adelaide Carpenter and there *F are details about the stocks that need to make it into FB. *F 1. Meiotic mutants *F We now have stocks of mei-1601, mei-381 and mei-991. None of these *F have FB entries. All come from Baker and Carpenter, 1972 (FBrf0023919) *F which I don't think has been curated (I think there are quite a few other *F mei mutations in the paper that aren't in FB). The stocks are: *F 4878 y1 mei-1601/C(1)DX, y1 f1/Dp(1;Y)y+; spapol *F 4881 y1 mei-381/C(1)DX, y1 f1/Dp(1;Y)y+; spapol *F 4882 y1 mei-991/C(1)DX, y1 f1/Dp(1;Y)y+; spapol *F 2. Jag *F The stock Adelaide sent is: *F 4913 Df(2R)17l, cn1 Jag2/CyO 2 041F03-04;042A03-09 *F Synonym : Df(2R)171 *F The missing wing end mentioned in FBab0022235 is most likely a second *F allele of Jag based on phenotype and map position; however, allelism has *F not been checked by complementation (does Jag1 even still exist?). *F 3. TM3, Sb D Ser ry balancer variant *F The stock is: *F 4910 cn1; TM3, In(3LR)D6, D6 Sb1 Ser1 ryRK/mwh1 *F P{ry+t7.2=ry11}l(3)ry31 ry506 *F Needs to be listed under TM3 entry. *F 4. l(3)78Ad1 *F 4904 Ab(3L;het)ME178, mwh1 l(3)78Ad1 red1 e1/TM2 *F This aberration fails to complement Dfs of 78A. l(3)78Ad1 gives a name *F to the mutation. *F 5. l(3)78Ae1 *F 4875 T(2;3)ME21, mwh1 l(3)78Ae1 red1 e1/TM3, Sb1 ryRK *F This T(2;3) fails to complement Dfs of 78A. l(3)78Ae1 gives a name to *F the mutation. *F 6. l(3)ry32 *F 4909 cn1; T(3;4)11d, mwh1 l(3)ry32 e1 ry506/TM3, Sb1 Ser1 *F This one is problematic. The T(3;4) comes from an experiment that mutated *F the ry+ in P{ry+t7.2=ry11}l(3)ry31 by X rays. T(3;4)11d (78A5-6;het) *F fails to complement Dfs of 78A, but there may be no second lethal mutation *F in addition to the lethal at l(3)ry3. Adelaide says that *F P{ry+t7.2=ry11}l(3)ry31 may not map to 78C as described. *F So how should we deal with this? We could say that the only mutation is an *F X ray-induced ry- derivative of P{ry+t7.2=ry11}l(3)ry31 called *F l(3)ry32, but that would entail moving l(3)ry3 from 78C to 78A. *F Alternatively, we could create a l(3)78Af1 mutation for the lethal in 78A *F (associated with the T(2;3) breakpoint) and create a l(3)ry32 for the *F ry- derivative. Please advise. *F 7. Dp(2;3)Me3 *F 4903 Dp(2;3)Me3/DcxF 060D01+;060E02-3;063A02+ *F Transposition of 60D2 to 60E1,2 into interband between 63A2 and 63A3. New *F order: 61A1-63A2/60E2-60D2/63A3-100F. Me3 phenotype weaker than Me1 *F and unscoreable in cn. Me3 lethal over Me1. *F 8. l(2)82Fk1 *F 4870 mwh1 l(3)82Fk1 red1 e4/TM3, Sb1 Ser1 *F ENU. leaky late pupal/eclosion lethal. *F Thanks for giving this your attention. *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 812-855-5782 *F Indiana University *F Bloomington, IN 47405-6801 *F From rd120@gen.cam.ac.uk Mon Nov 30 16:46:26 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 30 Nov 1998 16:46:26 +0000 *F To: kcook@bio.indiana.edu *F Subject: Re: Info on Adelaide Carpenter's stocks *F Cc: rd120@gen.cam.ac.uk, atc12@mole.bio.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 30 Nov 1998 16:47:48 +0000 *F Content-Length: 3386 *F Dear Kevin (or maybe Adelaide - I don't know who wrote which bit of this), *F >2. Jag *F >The stock Adelaide sent is: *F >4913 Df(2R)17l, cn[1] Jag[2]/CyO 2 041F03-04;042A03-09 *F > *F >The missing wing end mentioned in FBab0022235 is most likely a second *F >allele of Jag based on phenotype and map position; however, allelism has *F >not been checked by complementation (does Jag[1] even still exist?). *F I am not sure what you want me to record here. Is it that Df(2R)17l *F deletes Jag? If so, there is no need to infer an allele of Jag, I *F would simply record that Df(2R)17l 'deletes or disrupts' Jag. *F Alternatively one of the breaks might break IN Jag, in which case an *F allele would be in order, as well as the 'Df(2R)17l 'deletes or *F disrupts' Jag' statement. Alternatively again Df(2R)17l might have nothing *F to do with the jagged phenotype, in which case 'Df(2R)17l 'does not *F deletes or disrupt' Jag' and Jag[2] simply arose at the same time as *F Df(2R)17l. *F One reasonable (?) route is to record that 'Df(2R)17l 'deletes or *F disrupts' Jag' and NOT make an allele. An even safer route would be to *F make no map statements at all but simply record the phenotype - in fact *F that is the way that this data has been dealt with in the past. That *F is what I have done again (being a cautious sort). It all depends on *F how confident you are that the phenotype maps to the Jagged locus (your *F call not mine). *F Haven't a clue whether Jag[1] exists. It's not cropped up in any *F publication during FlyBase curation, which is suggestive that it is *F at least not very well .... *F >3. TM3, Sb D Ser ry balancer variant *F >The stock is: *F >4910 cn[1]; TM3, In(3LR)D[6], D[6] Sb[1] Ser[1] ry[RK]/mwh[1] *F >P{ry[+t7.2]=ry11}l(3)ry3[1] ry[506] *F > *F >Needs to be listed under TM3 entry. *F I have generated a balancer variant, TM3-D[6], Bal_short_name: TM3, *F Sb[1] D[6] Ser[1] ry[RK], under In(3LR)D[6], which is where it belongs, *F being a variant of a distinct aberration. *F >6. l(3)ry3[2] *F > *F >4909 cn[1]; T(3;4)11d, mwh[1] l(3)ry3[2] e[1] ry[506]/TM3, Sb[1] Ser[1] *F > *F >This one is problematic. The T(3;4) comes from an experiment that mutated *F >the ry[+] in P{ry[+t7.2]=ry11}l(3)ry3[1] by X rays. T(3;4)11d (78A5-6;het) *F >fails to complement Dfs of 78A, but there may be no second lethal mutation *F >in addition to the lethal at l(3)ry3. Adelaide says that *F >P{ry[+t7.2]=ry11}l(3)ry3[1] may not map to 78C as described. *F > *F >So how should we deal with this? We could say that the only mutation is an *F >X ray-induced ry[-] derivative of P{ry[+t7.2]=ry11}l(3)ry3[1] called *F >l(3)ry3[2], but that would entail moving l(3)ry3 from 78C to 78A. *F >Alternatively, we could create a l(3)78Af[1] mutation for the lethal in 78A *F >(associated with the T(2;3) breakpoint) and create a l(3)ry3[2] for the *F >ry[-] derivative. Please advise. *F In the absence of further info I would prefer not to invoke any new *F loci/alleles. There is just not enough here to go on, to choose *F between the various possibilities. If the translocation breaks at 78A, *F why is it ry[-]? If the translocation breaks at ry (in *F P{ry[+t7.2]=ry11}) why does the chromosome fail to comp Dfs at 78A? *F Either way we do not necessarily need a new allele of l(3)ry3. Are we *F absolutely sure that 78A6-78C are still there? We probably are .... *F Rachel. *F From atc12@mole.bio.cam.ac.uk Mon Nov 30 20:04:39 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 30 Nov 1998 20:04:39 +0000 *F Date: Mon, 30 Nov 1998 20:06:01 +0000 (GMT) *F From: Adelaide T C Carpenter *F To: Rachel Drysdale *F cc: Kevin Cook *F Subject: Re: Info on Adelaide Carpenter's stocks *F MIME-Version: 1.0 *F Dear Rachel, *F Re Jag (Df(2R)17l): I agree with conservatism (after all, that's *F why I didn't name the Df as Jag-something!). This is one of the many *F incidental things I picked up: I've done nothing but note the phenotype, *F done the cytology, and don't expect to do more. However, however this is *F handled there should be some sort of pointer under the Jagged gene entry *F \-- so that someone who \*does* become interested in Jag knows about this *F stock so they can order it and do the legwork on it! *F We didn't notice any haplo-sensitive phenotype in The Great *F Translocation Hunt down there, so presumably the dominant phenotype is due *F to a neomorphic mutation caused by one or both breakpoints -- or, of *F course, by an extraneous hit somewhere else on 2. Again, someone who *F cares would have to do an experiment. *F Re that damned P{ry[+t7.2]=ry11}l(3)ry3: I know a few more things *F about it: sorry, this issue came up back when my stocklist was being *F curated, I thought I'd given you all the necessary information! but *F apparently not -- and there was one additional cross I had to do (and *F did). 1) The original P insert is viable over deficiencies of 78C; 2) *F The original P insert is lethal over deficiencies of 78A; these two *F observations strongly suggest that the lethality, if not the P, is in 78A. *F Furthermore, 3) the X-ray induced knockout of the ry[+] of that insert *F has its breakpoint in 78A. This strongly suggests that the original *F insert was in 78A, not C! However, I have not bothered to check its *F location by in situ. *F Moreover, it's worth noting that this is one of the regions that *F Bridges revised; if whoever did its in situ cytology used the wrong map, *F and didn't try to to get its position closer than the nearest major bands, *F '78C' is consistent with 78A5-6. I think there's enough evidence here to *F move P{ry[+t7.2]=ry11}l(3)ry3 to 78A5-6. *F Hope this clears up the confusion! *F Cheers, Adelaide *F From rd120@gen.cam.ac.uk Tue Dec 01 14:44:15 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 1 Dec 1998 14:44:15 +0000 *F To: rd120@gen.cam.ac.uk, atc12@mole.bio.cam.ac.uk *F Subject: Re: Info on Adelaide Carpenter's stocks *F Cc: kcook@bio.indiana.edu *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Tue, 1 Dec 1998 14:45:38 +0000 *F Content-Length: 1335 *F Hi Adelaide and Kevin, *F thanks for your mail. *F Re: l(3)ry3 *F In view of what Adelaide has added about P{ry[+t7.2]=ry11}l(3)ry3 I *F have arranged for the cytology reported in FBrf0054167 (Berg and *F Spradling, 1991) to be marked as suspect which will prevent it from *F being used in map generation, and I have generated a new allele of *F l(3)ry3, l(3)ry3[2] (caused by T(3;4)11d, progenitor l(3)ry3[1]). *F Since T(3;4)11d has a 78A5-6 break, the new reported location for *F l(3)ry3 will become 78A5-6. The gene report for l(3)ry3 will include *F the following comment: *F 'l(3)ry3[1] is viable over deficiencies of 78C and lethal over *F deficiencies of 78A.' *F So Kevin, to tweak what you said, a better thing would be to say *F >cn[1]; T(3;4)11d, mwh[1] l(3)ry3[2] e[1] ry[506]/TM3, *F >Sb[1] Ser[1] *F > *F >with a comment that says 'The P{ry[+t7.2]=ry11}l(3)ry3[1] progenitor *F >chromosome has been mutated to ry[-] in this stock; T(3;4)11d fails to *F >complement Dfs of 78A.' *F Re: Df(2R)17l *F I have made this note under 'comments on cytology' for the Jag gene: *F 'Df(2R)17l shows a phenotype very similar to that reported for Jag[1], *F suggesting that Jag may be disrupted by the deficiency.' *F and under Df(2R)17l I have recorded that *F 'The missing wing end phenotype is possibly the result of disruption of *F Jag.' *F I am happy with all that - hope you are too! *F Rachel. # *U FBrf0106082 *a Laverty *b T. *t 1998.12.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 03 17:43:51 1998 *F To: rd120@gen.cam.ac.uk *F Subject: Df(2L)S2590 info *F Hi Rachel-- *F Here is a little information about Df(2L)S2590 that Todd said I could *F forward to you. We will be incorporating this Df strain into the B'ton *F collection soon. *F Best regards, *F Kevin *F >Return-Path: *F >Date: Wed, 2 Dec 1998 17:00:38 -0800 *F >To: Kevin Cook *F >From: tlaverty@uclink4.berkeley.edu (Todd Laverty) *F >Subject: Re: l(3)L2249 *F > *F >Hi Kevin, *F >Yes, we have l(3)L2249 and will send both you and Jeff a copy. *F > *F >On another note, I have some more info on Df(2L)S2590 that you were talking *F >to Amy about recently. I'm not sure what she has told you but I'll tell *F >you what I know and what I recently found out. First, it is not a Scott *F >line, it came from a Ras screen in the lab and was X-ray induced. The *F >cytology of the breakpoints 23D2; 23E3 should be pretty accurate since the *F >deficiency junction was cloned from the S2590 stock, labeled and hybridized *F >back to wild type chromosomes. The result was two signals, one at 23D1-2 *F >and the other 23E3-4. This was done with two independent clones. *F >Now this is what I learned recently and where it gets confusing for me and *F >clouds up the picture a bit. The deficiency junction was sequenced and it *F >breaks in the toucan gene on the 23D1-2 end, were the last ~4300 bp is *F >missing in S2590 stock. The confusing part is the gene mad, which is *F >distal to toucan, is also missing in S2590. *F > *F >l(2)k00237, which is a P allele of mad, fails to complement Df(2L)S2590 and *F >a southern with S2590 genomic DNA with a mad cDNA probe, shows mad is *F >missing in S2590. This implies that there is a small (not cytologically *F >visible) second aberration on the D(2L)S2590 chromosome. All this work was *F >done by Allan Wong, an undergraduate in the lab, and Marc Therrien, a *F >postdoc in the lab. They have stop working on this project since what *F >appeared at first to be a simple side project had grown in to a more *F >complicated project they chose not to pursue. *F > *F >As always, if you have any question, let me know and I try and find the *F answers. *F >Take care, *F >Todd *F > *F >Todd Laverty *F >Rubin Lab *F >tlaverty@uclink4.berkeley.edu # *U FBrf0106083 *a Roote *b J. *c Y. *d Zhang *t 1998.12.8 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Dec 08 11:52:05 1998 *F To: rd120@mole.bio.cam.ac.uk *F Subject: pers comm to FB *F Dear Rachel, *F Df(1)RJ7 obtained from D. Nash, Df(1)9B2;9E3.4 (cytology by Y. Zhang), *F deletes ras but not l(1)9Ec. *F John *F ___________________________________________________________________ *F John Roote *F Michael Ashburner's lab Tel: 44 1223 333982 *F Department of Genetics Fax: 44 1223 333992 *F University of Cambridge email: j.roote@gen.cam.ac.uk *F Downing Street *F Cambridge *F CB2 3EH *F UK # *U FBrf0106085 *a Auld *b V. *c J. *d Roote *t 1998.12.14 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Dec 14 18:17:05 1998 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 14 Dec 1998 18:17:05 +0000 *F Mime-Version: 1.0 *F Date: Mon, 14 Dec 1998 18:17:49 +0100 *F To: rd120@mole.bio.cam.ac.uk *F From: John Roote *F Subject: Re: Gli *F rachel, *F a little extra data on Gli alleles. This email from Vanessa Auld says that *F AE2 and J29 are viable alleles (not stated in FB). *F Also the excisions of AE2 - GliAE2&Dgr;45 and *F GliAE2&Dgr;4b - are lethal alleles derived from AE2; *F and GliAE2&Dgr;4a is a viable allele. *F J *F >Envelope-to: jr32@mole.bio.cam.ac.uk *F >X-Sender: auld@mailhost.zoology.ubc.ca *F >Mime-Version: 1.0 *F >Date: Wed, 18 Nov 1998 10:01:54 -0800 *F >To: John Roote *F >From: Vanessa Auld *F >Subject: Re: Gli *F > *F >Dear John, *F >The P induced Gli alleles were not lethal insertions. They are totally *F >viable because they are inserted in the first intron of the gene. The P *F >elements are the PlacZ, w+ element for AE2, J29 and the PlacZ, ry+ *F >element for rL82. I made a series of deletion alleles of gliotactin using *F >the Pelement insertion and these deletions were lethal and protein nulls *F >(called delta45, delta4b). As well at the same time I made a precise *F >excision using the same P element insertion and this allele is viable and *F >called delta4a. *F > *F >Hope this is helpful, *F >Vanessa. *F > *F > *F >==================================================================== *F >Dept. Zoology *F >6270 University Blvd. *F >University of British Columbia *F >Vancouver, B.C. *F >Canada V6T 1Z4 *F > *F >office: (604) 822-1977 *F >lab: (604) 822-0697 *F >fax: (604) 822-2416 *F > *F >email: auld@zoology.ubc.ca *F >web page: http://cord.ubc.ca/auld *F >==================================================================== *F > # *U FBrf0106086 *a Bloomington Drosophila Stock Center *b ?. *t 1998.12.15 *T personal communication to FlyBase *u *F Personal communication from: Andrea Brand, University of Cambridge *F To: Bloomington Drosophila Stock Center (via Thom Kaufman) *F Subject: P{GawB}AB1 *F Dated: 5 July 1995 *F *F Background: In the preparation of this personal communication Bloomington has used valid FlyBase nomenclature for *F the insertion symbol. The insertion phenotype has been inferred from the genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{GawB}AB1 *F Insertion phenotype viable | fertile *F Localization unknown (not on X) *F Other/Comments GAL4 in salivary gland, basal expression of P{GawB} *F *F # *U FBrf0106089 *a Michelson *b A.M. *t 1998.12.16 *T personal communication to FlyBase *u *F From michelson@rascal.med.harvard.edu Wed Dec 16 23:40:41 1998 *F To: rd120@gen.cam.ac.uk *F Subject: hello again + Flybase info. *F Dear Rachel, *F . *F . *F I'm writing now with a Flybase update. I *F wasn't sure who would be the best person to contact, but I figured that you *F could at least pass on the information to the appropriate party. It *F relates to a gene that we previously reported and that is listed in Flybase *F as 'heartbroken' (hbr). The original entry was based on an abstract in the *F 1997 U.S. Drosophila Conference. We have since published a paper on this *F gene -- Development 125, 4379-4389 (1998) but it's so recent that I have *F just seen it on the journal's website, not in print form. What is more *F interesting and not yet in the literature is that hbr is allelic to *F 'downstream of FGF receptor' (dof), a gene that was independently *F characterized (and in their case cloned) by Markus Affolter and Maria *F Leptin. The reference for their paper is Molecular Cell 2, 515-525 (1998). *F I checked Flybase but didn't see dof listed yet. When the curator gets *F around to this, I just wanted them to know that dof and hbr are indeed *F allelic and should be listed as such. (We exchanged alleles with Maria and *F Markus some time ago and all the labs have confirmed the results of the *F complementation tests.) *F . *F . *F . *F Alan *F Alan M. Michelson *F Howard Hughes Medical Institute *F Brigham and Women's Hospital *F 20 Shattuck Street *F Boston, MA 02115 *F Tel.: (617) 732-7411 (Office) 732-5208 (Lab.) 738-5575 (FAX) *F E-mail: michelson@rascal.med.harvard.edu # *U FBrf0106090 *a Whitfield *b E. *t 1999.1.7 *T personal communication to FlyBase *u *F From eleanor@ebi.ac.uk Thu Jan 07 11:21:13 1999 *F To: 'Rachel Drysdale (Genetics)' , *F Subject: FBrf0047944 *F Hi, *F This is one correction to make please for the EMBO paper! *F In gene l(3)89Ee you have an attributed comment (taken from the Web): *F >Other information de Lorenzi et al., 1988: The *F >pH200 transcript may stem from the l(3)89Ee gene. *F and I now know that, and can say with confidence, pH200 in fact codes *F for Ahcy89E. *F At present FBrf0047944 is not referenced under Ahcy89E. *F thanks, *F Ele # *U FBrf0106091 *a Portin *b P. *c M. *d Rantanen *t 1998.12.18 *T personal communication to FlyBase *u Heterologous disjunction of the extra Y chromosome and minichromosomes in the female Drosophila melanogaster meiosis is roughly but not completely dependent on the (heterochromatic) length of the minichromosome. *F Archived. # *U FBrf0106092 *a Peifer *b M. *t 1999.1.8 *T personal communication to FlyBase *u *F From bdgp-owner@fruitfly.bdgp.berkeley.edu Fri Jan 08 18:48:44 1999 *F From: Mark Peifer *F To: bdgp@fruitfly.bdgp.berkeley.edu *F Subject: bdgp addendum to P entry *F Hi there, *F I just thought you might want to add to the report for EP(3)1225 the *F fact that it is inserted in an intron of the puckered gene. I have *F included the match to the coding sequence of puckered below. *F >EP(3)1225 AQ025202 inserted at base 179 Both 5' and 3' ends of P element *F Inverse PCR *F Length = 244 *F Plus Strand HSPs: *F Score = 323 (48.5 bits), Expect = 3.3e-10, P = 3.3e-10 *F Identities = 123/176 (69%), Positives = 123/176 (69%), Strand = Plus / *F Plus *F Query: 580 *F GCGCCGTGTCTTCTGTTATTTATGCCAATCCAAAATACCAACAACAACAATCGCATTGGT 639 *F GCGCCGTGTCTTCTGTTATTTATGCCAATCCAAAATACCAACAACAACAATCGCATTGGT *F Sbjct: 1 *F GCGCCGTGTCTTCTGTTATTTATGCCAATCCAAAATACCAACAACAACAATCGCATTGGT 60 *F Query: 640 *F GCCAA-T-CAGAAGGA--CTATCCCAACAAGCGG-TCCAGGGAGAATCTGGCATGTGACG 694 *F G AA T CA AA GA C A AA AA C TC A GA AAT G AT GA *F Sbjct: 61 *F GGTAAGTACAAAAAGAAGCGAAAAAAAGAATCAAATCAACAGA-AATAAGCAATAAGAAA 119 *F Query: 695 AAGTG-ACA-TCGACAACGTCA-TCAT-CCACGGCAATGAACGGCGGC-GGACGGACG *F 747 *F AA G ACA T A A CA TCA CCA AA GA CGG G C GG GGACG *F Sbjct: 120 AATCGTACAATTTAAGATTACACTCAAACCAATCAAAAGAGCGG-GACAGGGAGGACG *F 176 *F Sincerely yours, *F Mark Peifer *F Department of Biology *F Coker Hall; CB#3280 *F University of North Carolina at Chapel Hill *F Chapel Hill NC 27599-3280 *F 919-962-2271 919-962-1625 (FAX) # *U FBrf0106094 *a Rong *b Y. *t 1999.1.20 *T personal communication to FlyBase *u *F From GolicLab@bioscience.utah.edu Subject: Re: FWD>Helping FlyBase *F To: 'Genetics' *F Dear Rachel: *F All the revertants deleted the proximal junction of *F In(3R)pugD. The two break points for In(3R)pugD are 86C3-4 and 95D1-6. The *F deleted segments of the revertants are as followed: *F rv7: 85E15-pugD-95C8. *F rv1: 86A1-pugD-95B3. *F rv17*: 86A8-pugD-94F3. *F rv10: 86B6-pugD-94F4. *F rv5: 86C3-4-pugD-95C8. *F rv15**: 86C3-4-pugD-95C5. *F rv2: 86C3-4-pugD-95B4. *F rv6: 86C3-4-pugD-95A5. *F rv4: 86C3-4-pugD-94F3. *F \*: the corresponding region is translocated to the Y chromosome giving rise *F to Dp(3;Y)pugD. *F \**: also contains an In(3R)82E3-4;91C3-4. *F Please feel free to contact me for further questions. *F Sincerely, Yikang Rong *F \-------------------------------------- *F Date: 1/20/99 10:21 AM *F To: GolicLab *F From: Golic *F Yikang - please respond. *F \-------------------------------------- *F Date: 1/20/99 8:12 AM *F From: Genetics *F Dear Kent, *F I have just started curating your Genetics paper *F Rong and Golic, 1998, Genetics 150(4): 1551--1566. *F Dominant defects ..... *F I notice that Figure 4 includes several cytologically defined *F deficiency revertants but unfortunately I do not see text descriptions *F of the break points. This means that the cytology is impossible for us *F to capture for FlyBase. (We do not infer cytologies from diagrams, as *F a policy, since it is too prone to differences of interpretation *F between author and curator). *F Would you be able to tell me with breakpoints for the following *F chromosomes from Figure 4? The aberration records that result in *F FlyBase will be so much more useful with the breakpoint information. *F rv7 *F rv1 *F rv17 *F rv10 *F rv5 *F rv15 *F rv2 *F rv6 *F rv4 *F With best wishes, *F Rachel. # *U FBrf0106095 *a Schwartz *b Y. *t 1999.1.18 *T personal communication to FlyBase *u *F From ys224@mole.bio.cam.ac.uk Mon Jan 18 12:27:04 1999 *F To: r.drysdale@gen.cam.ac.uk *F Subject: dor allels *F Hi, Rachel. *F Please, find enclosed the table of dor allels I made using the FlyBase *F information. *F I would like to inform you, that: *F dor8 = l(1)7 is the late larvae lethal as in this case dor9 and dor10 have *F to be. *F dor29 is late larvae lethal too. *F There is one allele dor lt148 I can't find in your data base. It is *F mentioned at least in Belyaeva E.S. et al. 1982. DIS 58:184-190 and *F Demakova O.V. et al. 1988. DIS 67: 21-27. *F Cheers, Yuri. *F From rd120@gen.cam.ac.uk Thu Jan 21 16:47:09 1999 *F To: ys224@mole.bio.cam.ac.uk *F Subject: Re: dor allels *F Hi Yuri, *F I am just dealing with your message. Just to be sure: *F >dor8 = l(1)7 is the late larvae lethal as in this case dor9 and dor10 have *F to be. *F are you saying that dor8, dor8 and dor8 are all late larval lethals? *F >There is one allele dor lt148 I can't find in your data base. *F We have this allele as 'dort148' with a synonym of 'dorlt148' but I *F see (now that we have been back to the old papers) that dorlt148 was *F used as a symbol before dort148 so I will edit the files so that *F dorlt148 becomes valid and dort148 secondary. *F Unfortunately you won't see these changes on the public server for a *F few weeks but they will be made in the master files after I get your *F reply. *F Thanks! *F Rachel. *F From ys224@mole.bio.cam.ac.uk Thu Jan 21 21:21:50 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: dor allels *F Hi Rachel, *F >are you saying that dor8, dor8 and dor8 are all late larval lethals? *F Yes, ofcaurse, all of them are late larvae lethals :). *F In fact, I'd never seen dor9 and dor10, but according to FlyBase *F description they have to be like dor8, so late larvae lethals. Also Elena *F Belyaeva wrote me recently that there are no adult lethal dor mutations *F known. *F Cheers, Yuri. # *U FBrf0106096 *a Roote *b J. *t 1999.1.18 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Mon Jan 18 17:49:19 1999 *F To: rd120@mole.bio.cam.ac.uk *F Subject: Scorv18 *F Hello Rach, *F A quickie: 1) that Scorv18 is broken in BicC (Associated Allele *F BicCSco-rv18) and 2) deletes beat (Genetic data about other aber. In *F Df(2L)Scorv18/Df(2L)RM5 transheterozygotes (beat- BicC-)) cannot both be *F true. *F The gene order is BicC beat. RM5 removes both and my genetics suggests *F that rv18 also removes both (semi-lethal with beatdpp36). i.e. the *F associated allele looks wrong. *F J # *U FBrf0106097 *a Salveterra *b P. *t 1999.1.20 *T personal communication to FlyBase *u *F From celniker@currant.lbl.gov Wed Jan 20 12:36:02 1999 *F To: gelbart@morgan.harvard.edu *F Dear Bill, *F Here is the response from Paul Salvaterra. I hope Flybase can be updated. *F Thanks Sue *F Dear Susan, *F Sorry for the late reply but I have been out of town and out of touch with my *F email. You are correct. We missinterpreted our in situ localization of the Nrv *F genes. They are at 27AB. We have recently published the corrected in situs in *F the last DIS (Williamson et al., Drosophila Information Services). By the way *F the Genome Project has been a great help to our research and I thank all those *F involved in this effort. *F Paul Salvaterra *F ____________________Reply Separator____________________ *F Subject: Nrv localizations *F Author: 'Susan E. Celniker' *F Date: 1/12/99 10:26 AM *F Dear Paul, *F >From our genomic sequencing it appears that the Nervana genes were *F mis-localized. Is it possible the insitu were incorrectly interpreted? *F We find they reside on DNA that maps to 27AB and not 92CD. Thank *F you in advance for confirming this localization. *F Susan Celniker *F Staff Scientist *F Co-Director Berkeley Drosophila Genome Project Sequencing Center *F (510)486-6258 # *U FBrf0106226 *a Biggin *b M. *t 1999.2.5 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Mon Feb 01 11:49:27 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 1 Feb 1999 11:49:27 +0000 *F To: mark.biggin@yale.edu *F Subject: Help FlyBase please *F Cc: ma11@gen.cam.ac.uk, gm119@gen.cam.ac.uk, ag24@gen.cam.ac.uk *F Content-Type: X-sun-attachment *F From: Michael Ashburner (Genetics) *F Date: Mon, 1 Feb 1999 11:51:36 +0000 *F Content-Length: 5148 *F FlyBase is curating your Nov Development paper (actually I am checking *F the curation done before Xmas). *F Some ''homology'' questions: *F If you can give me more data onall the matches to non-fly genes *F ie the organism and the accession number of the matched sequence, *F then I could improve the curation. *F ====== *F What is HN1 ? *F AI124289 *F DE melanogaster cDNA 5' similar to HN1, mRNA sequence. *F ====== *F Is this one of the Ef1alphas ? and not one of those already in FB *F AI124295 *F DE melanogaster cDNA 5' similar to eIF-1A, mRNA sequence. *F FB knows of *F Ef1&agr;100E *F Ef1&agr;100E *F ====== *F 1.69 was not in the Table, was this just an oversight ? It is *F AI124285 *F ====== *F What is VAP-33 *F AI124321 *F DE melanogaster cDNA 5' similar to VAP-33, mRNA sequence. *F ====== *F AI124338 *F DE melanogaster cDNA 5' similar to E(spl)m7/m8, mRNA sequence. *F do you know if this is E(spl) (aka m8) (Genbank X16549, X16550, X16553) *F or HLHm7 (Genbank U13069, X16553) ?? *F ====== *F I cannot find an accession number for clone 1.39, clone 1.62, clone 2.1, *F clone 2.40 *F I attach the annotation FB has now added to these records. *F \*a is the FB gene symbol, \*g the accession number, \*d any functional *F inference (on new genes only) \*J any structural inference (again on new *F genes only), \*j similar genes in other species (on new genes only). *F I would be grateful (very) if you could look this over and make sure I *F have not made errors. We have given the new genes temporary symbols *F anon-* where \* is your clone number. *F ==================================================================== *F Best wishes *F Michael *F Known genes: *F \*a sta *F \*g AI124333 *F \# *F \*a HmgZ *F \*g AI124283 *F \# *F \*a Bsg25A *F \*g AI124337 *F \# *F \*a HmgD *F \*g AI124252 *F \# *F \*a &agr;Tub84B *F \*g AI124284 *F \*g AI124313 *F \# *F \*a E(spl) *F \*g AI124338 *F \# *F \*a nmo *F \*g AI124322 *F \# *F \*a N *F \*g AI124254 *F \# *F \*i EF-1&agr;F *F \*g AI124257 *F \# *F \*a G&bgr;13F *F \*g AI124260 *F \# *F \*a Tis11 *F \*g AI124263 *F \# *F \*a scrt *F \*g AI124265 *F \# *F \*a dally *F \*g AI124335 *F \# *F \*a Ef2b *F \*g AI124274 *F \# *F \*a Ef1&agr;48D *F \*g AI124325 *F \# *F \*a HLHm&ggr; *F \*g AI124279 *F \# *F \*a fs(1)h *F \*g AI124291 *F \# *F \*a Wnt4 *F \*g AI124296 *F \# *F \*a ttk *F \*g AI124297 *F \# *F \*a Crc *F \*g AI124298 *F \*g AI124304 *F \# *F \*a awd *F \*g AI124310 *F \# *F \*a stg *F \*g AI124307 *F \# *F \*a pnut *F \*g AI124305 *F \# *F \*a Hsp83 *F \*g AI124331 *F \# *F \*a EB1 *F \*g AI124315 *F \*g AI124330 *F \# *F \*a RpS3A *F \*g AI124312 *F \# *F \*a Cys *F \*g AI124316 *F \# *F \*a msi *F \*g AI124317 *F \# *F \*a anon-1.1 *F \*g AI124249 *F \*d signal sequence receptor &bgr; \like *F \# *F \*a anon-1.11 *F \*g AI124251 *F \# *F \*a anon-1.13 *F \*g AI124334 *F \# *F \*a anon-1.14 *F \*g AI124255 *F \# *F \*a anon-1.16 *F \*g AI124256 *F \# *F \*a anon-1.18 *F \*d RNA helicase *F \*g AI124258 *F \# *F \*a anon-1.21 *F \*d pre-mRNA splicing helicase *F \*g AI124259 *F \# *F \*a anon-1.24 *F \*g AI124261 *F \# *F \*a anon-1.25 *F \*g AI124262 *F \# *F \*a anon-1.29 *F \*g AI124264 *F \# *F \*a anon-1.3 *F \*g AI124250 *F \# *F \*a anon-1.32 *F \*g AI124266 *F \# *F \*a anon-1.34 *F \*g AI124267 *F \# *F \*a anon-1.35 *F \*g AI124268 *F \# *F \*a anon-1.37 *F \*g AI124269 *F \# *F \*a anon-1.38 *F \*g AI124270 *F \# *F \*a anon-1.39 *F \# *F \*a anon-1.4 *F \*g AI124251 *F \# *F \*a anon-1.40 *F \*g AI124271 *F \# *F \*a anon-1.41 *F \*g AI124272 *F \# *F \*a anon-1.43 *F \*g AI124273 *F \# *F \*a anon-1.45 *F \*j species == Saccharomyces cerevisiae; gene == EMP24; SGDID:L0000549 *F \*d transport vesicle membrane protein \? *F \*g AI124275 *F \# *F \*a anon-1.46 *F \*g AI124276 *F \# *F \*a anon-1.47 *F \*g AI124277 *F \# *F \*a anon-1.48 *F \*j species == Saccharomyces cerevisiae; gene == SAR1; SGDID:L0001801 *F \*g AI124278 *F \*d GTP-binding protein \? *F \# *F \*a anon-1.52 *F \*g AI124280 *F \# *F \*a anon-1.53 *F \*g AI124281 *F \# *F \*a anon-1.56 *F \*g AI124282 *F \*d ribosomal-protein-S8 \like *F \# *F \*a anon-1.62 *F \# *F \*a anon-1.66 *F \*j species == Saccharomyces cerevisiae; gene == CDC47; SGDID:L0002760 *F \*g AI124336 *F \# *F \*a anon-1.69 *F \*g AI124285 *F \# *F \*a anon-1.72 *F \*g AI124286 *F \# *F \*a anon-1.74 *F \*g AI124287 *F \# *F \*a anon-1.75 *F \*g AI124288 *F \# *F \*a anon-1.76 *F \*g AI124289 *F \# *F \*a anon-1.78 *F \*g AI124339 *F \# *F \*a anon-1.79 *F \*g AI124290 *F \# *F \*a anon-1.80 *F \*d calponin \? *F \*g AI124340 *F \# *F \*a anon-1.82 *F \*g AI124292 *F \# *F \*a anon-1.83 *F \*g AI124293 *F \# *F \*a anon-2.1 *F \# *F \*a anon-2.12 *F \*g AI124299 *F \# *F \*a anon-2.13 *F \*g AI124300 *F \# *F \*a anon-2.14 *F \*g AI124301 *F \# *F \*a anon-2.15 *F \*F methionine--tRNA ligase == EC 6.1.1.10 \? *F \*g AI124302 *F \# *F \*a anon-2.16 *F \*g AI124303 *F \# *F \*a anon-2.19 *F \*g AI124306 *F \# *F \*a anon-2.2 *F \*g AI124294 *F \# *F \*a anon-2.23 *F \*g AI124308 *F \# *F \*a anon-2.24 *F \*g AI124309 *F \*d cyclin \? *F \# *F \*a anon-2.28 *F \*g AI124311 *F \# *F \*a anon-2.3 *F \*g AI124295 *F \# *F \*a anon-2.31 *F \*g AI124314 *F \# *F \*a anon-2.38 *F \*g AI124318 *F \# *F \*a anon-2.39 *F \*g AI124319 *F \# *F \*a anon-2.40 *F \# *F \*a anon-2.41 *F \*g AI124320 *F \# *F \*a anon-2.42 *F \*g AI124321 *F \# *F \*a anon-2.44 *F \*g AI124323 *F \# *F \*a anon-2.45 *F \*g AI124324 *F \# *F \*a anon-2.47 *F \*g AI124326 *F \# *F \*a anon-2.48 *F \*g AI124327 *F \*J Zinc finger protein. *F \# *F \*a anon-2.49 *F \*g AI124328 *F \# *F \*a anon-2.51 *F \*g AI124329 *F \# *F \*a anon-2.54 *F \*g AI124332 *F \# *F >From mark.biggin@yale.edu Mon Feb 01 19:40:02 1999 *F Return-path: *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 1 Feb 1999 19:40:02 +0000 *F Received: from lilac.csi.cam.ac.uk [131.111.8.44] (exim) *F by admin-sun1.gen.cam.ac.uk with smtp (Exim 1.58 #2) *F id 107PCU-0002P1-00; Mon, 1 Feb 1999 19:40:02 +0000 *F Received: from pantheon-po03.its.yale.edu ([130.132.143.34]) *F by lilac.csi.cam.ac.uk with esmtp (Exim 2.05 #3) *F id 107PEX-0004xs-00 *F for ma11@gen.cam.ac.uk; Mon, 1 Feb 1999 19:42:09 +0000 *F Received: from minerva.cis.yale.edu (biggin@minerva.cis.yale.edu [130.132.143.250]) *F by pantheon-po03.its.yale.edu (8.8.8/8.8.8) with ESMTP id OAA09369 *F for ; Mon, 1 Feb 1999 14:42:03 -0500 (EST) *F X-Authentication-Warning: minerva.cis.yale.edu: biggin owned process doing -bs *F Date: Mon, 1 Feb 1999 14:42:02 -0500 (EST) *F From: Mark Biggin *F X-Sender: biggin@minerva.cis.yale.edu *F To: Michael Ashburner *F Subject: Re: Help FlyBase please *F In-Reply-To: *F Message-ID: *F MIME-Version: 1.0 *F Status: RO *F X-Lines: 78 *F Dear Michael, *F I have three large folders with a section on each one of these *F homologies. Each section contains BLAST results, some medline abstracts, *F and a copy of at least one relevant paper. Is this information of any use *F to flybase? *F Also, please note that in Table 2, those genes given in italics *F are neither homologous to nor like the named Drosophila gene, they are *F identical to the named Drosophila gene (the DNA sequence match is *F perfect). *F In reply to your questions: *F 1. HN-1 is a mouse gene mapped to chromosome 11. It is expressed at *F highest levels in hemopoietic and brain tissues. The numbers associated *F with this sequence on the NCBI blast search are gi 1864165 or U90123. The *F paper describing HN1 is Tang et al (1997) Mammalian Genome 8, 165. (We *F did not note matches to other ESTs for which there was no biologically *F useful data. This clone is one of the most marginally informative *F homologies that we noted.) *F 2. eIF 1A is a translation initiation factor. It used to be called *F eIF-4C. There is no flybase entry for this gene. I think the Ef1alphas *F you refer to are translation elongation factors. (Fly base is using the *F old nomenclature, and still and calls the initiation factors it has *F entries for eIF-4B etc. Probably they should give both current and old *F names for all of these from now on. Your having the same problem I had: *F too many proteins with similar names.) Using BLAST we got matches to *F genes in Eukaryotes and Prokaryotes and Phage, but not in Drosophila. The *F numbers associated with the human homologue are gi 306725 or L18960 or sp *F P47813. The numbers associated with the S. cerevisiae homologue are *F Accession # 729814 or gi 533534 or sp P38912. A paper describing the *F Yeast protein is Wei et al (1995) J. Biol. Chem. 270, 22788-22794. *F 3. Clone 1.69 is actually one of the ribosomal RNAs. Two clones of the 99 *F clones that we performed in situs on gave no expression above background. *F Cone 1.69 was one of these clones. By chance, the probe was the correct *F strand to hybridize with the endogenous RNA, but we got no signal. I *F guess that the fast majority of the RNA is too crosslinked to the ribosome *F to be accessible. Since this is a technical artifact, I would prefer that *F you omit this ''result'' from Flybase. In the Table legend, we did note *F that two clones which gave no detectable expression were not included in *F the Table. *F 4. VAP-33 is a VAMP binding protein from aplysia that is required for *F Neurotransmitter release. (VAMP is an integral membrane protein that is *F something to do with synaptic vesicle fusion.) VAP-33Us numbers are: *F Accession 136258 or g1362584. A reference is Skehel et al (1995) Science *F 269 1580-1583. There is also a S. cerevisiae homologue (Accession 731508 *F or sp P40075) that is a suppressor of a choline sensitive mutant. *F 5. Clone 1.71 is E(spl) m7/m8 - aka m8 - Genbank X19553, not the other *F gene you mentioned. *F 6. The four clones without accession numbers did not give readable DNA *F sequence. Unfortunately, we never got the time to go back and redo these, *F so it is possible that we are missing a couple of homologies from our *F table. *F I have looked at the data you sent. I have only one correction *F anon 1.11 The accession number is wrong it should be AI124253, not *F AI124251 *F *F Thanks for the time you have taken *F Mark *F >From mark.biggin@yale.edu Fri Feb 05 01:17:59 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 5 Feb 1999 01:17:59 +0000 *F X-Authentication-Warning: minerva.cis.yale.edu: biggin owned process doing -bs *F Date: Thu, 4 Feb 1999 20:20:11 -0500 (EST) *F From: Mark Biggin *F X-Sender: biggin@minerva.cis.yale.edu *F To: Michael Ashburner *F Subject: Re: Help FlyBase please *F MIME-Version: 1.0 *F Dear Michael, *F I have tried to give you as many Swiss Prot numbers as possible. These are *F sp numbers. I am not sure what data bases the other numbers are from. *F All are protein sequences except for clone 1.56, which are DNA sequences. *F 1.1 Signal sequence receptor beta. There are many matches. Here are 3. *F sp P23438 Canis f. Signal sequence receptor beta *F sp P43308 Human Signal sequence receptor beta *F gi 1086874 C. elegans cDNA yk67f10.3 *F 1.18 RNA helicase. There are many matches. Here are 3. *F sp P53131 Yeast putative ATP dependent helicase *F sp P24384 Yeast pre mRNA splicing factor *F gi 2407195 (AF017153) M. musculus putative ATP dependent helicase *F 1.21 premRNA splicing helicase *F sp P32639 Yeast pre mRNA splicing helicase BRR2 *F 1.45 Emp24 Protein transport *F gi 1223892 (U418805) putative T1/ST2 receptor *F sp P49020 Cricetulus griseus COP coated vesicle membrane protein P24 *F gi 2275635 (AF014940) C. elegans ho,ologue of EMP24/GP25L *F 1.48 SAR-1 G protein. There are many matches. Here are 3. *F sp P36536 Mouse SARA GTP binding *F sp P20606 Yeast SAR1 GTP binding *F sp O01474 or Q01474 Arabidopsis *F 1.56 ribosomal protein S8. There are many matches. Here are 3. These are *F DNA sequences. *F emb X73829 MMRPS8 M. musculus *F gb U40799 CELF42C5 C. elegans cosmid *F emb X67247 HSRPS8 Human *F 1.66 CDC47/MCM2. There are many matches. Here are 4 *F sp P33993 Human DNA replication licensing factor *F sp P38132 Yeast cell division control protein *F gi 1469526 XMCM7 *F pir JC4580 mouse cell division protein *F 1.76 HNI *F gi 1864165 or U90123 mouse *F 1.80 Calponin. There are many matches. Here are 4 *F sp P51911 Human Calponin H1 *F sp O08092 Pig Calponin H1 *F sp O08091 Mouse Calponin H1 *F sp O08090 Rat Calponin H1 *F 2.3 eIF 1A translation initiation factor (AKA eIF-4C). There are many *F matches. Here are 3. *F sp P47813 Human eIF 1A *F sp P38912 S. cerevisiae eIF 1A *F sp P47815 Wheat eIF 1A *F 2.15 Met t-RNA synthetase. There are many matches. Here are 3. *F sp P00958 Yeast Met tRNA synthetase *F sp P00959 E. coli Met tRNA synthetase *F pir JC5224 Human methionine tRNA ligase *F 2.24 cyclin G2 *F gi 2228813 (AF005885) cyclin G2 M. musculus *F sp P51959 Human G2/mitotic specific cyclin G1 *F sp P39950 Rat G2/mitotic specific cyclin G1 *F 2.33 and 2.52 EB1. There are many matches. Here are 3. *F pir I52726 Human EB1 *F gi 1256434 (U51196) M. musculus EB1 homologue *F sp P40013 Yeast 38.4 KD hypothetical protein *F 2.42 VAP-33 *F pir A57245 VAP-33 *F sp P40075 S. cerevisiae *F gi 2394471 (AF024499) C. elegans homologue VAP-33 *F 2.48 A zinc finger. There are many matches. Here are 3. *F sp P51523 Human zinc finger *F sp P17141 Mouse zinc finger ZFP-37 *F sp P18727 Xenopus gastrula zinc finger protein XLCGF26 *F Thanks again *F Mark # *U FBrf0106235 *a Bloomington Drosophila Stock Center *b ?. *t 1999.1.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Jan 27 02:32:30 1999 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Bri *F Hi-- *F Please add *F Genetic Location 3- *F to Bri entry (FBgn0013754). The only allele is present on a TM6B balancer. *F Thanks, *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0106236 *a Bloomington Drosophila Stock Center *b ?. *t 1999.2.9 *T personal communication to FlyBase *u *F Personal communication from: Bloomington Drosophila Stock Center *F Background: Transposon that is in the Bloomington collection but is not *F in FlyBase *F Dated: 9th February 1999 *F Information communicated: *F Transposon P{w+mC=hs-ralA.N28} *F >From Denise Montell, contains mammalian ralA # *U FBrf0106309 *a Celniker *b S. *t 1998.11.30 *T personal communication to FlyBase *u *F >From nobody Mon Nov 30 16:09:20 1998 *F Date: Mon, 30 Nov 1998 16:09:17 -0500 (EST) *F From: celniker@currant.LBL.GOV (Sue Celniker) *F Subject: FlyBase Help Mail *F To: flybase-help@morgan.harvard.edu *F Content-Length: 531 *F celniker@bdgp.lbl.gov (Sue Celniker) sent the following *F comments to flybase-help: *F \------------------------------------------------------------ *F Dear Lynn and Bev, *F Our p1 D320 with accession \# AC005125 hits AF001784 not listed in *F Flybase and also hits U40077 with no location. The P1 maps *F to 30A7 - A8 by in situ hybridization. *F Sue *F \------------------------------------------------------------ *F Server protocol: HTTP/1.0 *F Remote host: gsue.lbl.gov *F Remote IP address: 131.243.192.35 # *U FBrf0106312 *a Certel *b K. *t 1999.2.9 *T personal communication to FlyBase *u *F >From kcertel@blue.weeg.uiowa.edu Tue Feb 09 16:08:36 1999 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 9 Feb 1999 16:08:36 +0000 *F Date: Tue, 9 Feb 1999 10:01:28 -0600 (CST) *F From: kaan certel *F X-Sender: kcertel@black.weeg.uiowa.edu *F To: Aubrey de Grey *F Subject: Re: Is ripcord the same gene as XBB70 Group VIII ? *F MIME-Version: 1.0 *F Dear Aubrey, *F You are absolutely correct, ripcord and l(3)65Da should be the *F same. I just checked the flybase records regarding l(3)65Da and it seems *F like this is the information about ripcord that was in our paper *F describing the original mutagenesis of the XBB70 region (before we named *F the complementation group VIII ripcord). After Mike identified these three *F complementation groups within XBB70, I started working on the ripcord *F stuff and presented a poster at the fly meeting. And that is the source of *F the ripcord info. So, these two should be merged to avoid redundancy. *F Please feel free to contact me if you have any further questions. *F Kaan *F On Mon, 8 Feb 1999, Aubrey de Grey wrote: *F > *F > Dear Kaan, *F > *F > I have just come across some communication between you and my colleague *F > Eleanor Whitfield from a few years ago, which gives some phenotypic *F > information about ripcord mutants and says that rip is within XBB70. *F > I see from Andersen et al., Genes & Dev. 9:123-137, that there are *F > only three known complementation groups in that deficiency, two of *F > which have real names already (vvl, aka dfr, and ple). Elimination *F > suggests that the third, ''Group VIII'', is ripcord. Is this so? If *F > so I will merge our records for the two genes and ripcord will get *F > some alleles at last! We currently list ''Group VIII'' as l(3)65Da. *F > *F > Cheers, Aubrey de Grey *F > FlyBase *F > *F \----- End Included Message ----- # *U FBrf0106723 *a Jongens *b T. *t 1999.2.10 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Jan 22 12:02:14 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 22 Jan 1999 12:02:14 +0000 *F To: jongens@mail.med.upenn.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 22 Jan 1999 12:04:14 +0000 *F Content-Length: 1478 *F Dear Dr. Jongens, *F I am curating your paper for FlyBase: *F Faulkner et al., 1998, Dev. Genet. 23(4): 264--274 *F I have a question about the P{lacW} insertion - pngcl - that you used *F to make excisions removing parts of the cmp44E gene. *F I would be grateful if you could tell me the identifier number of this *F line which you obtained from Gerald Rubin's lab, e.g. k02210, k03408 *F etc. as we probably already have a record of this line in FlyBase and I *F would like to be able to record the following information under the *F correct P{lacW} line. *F 1. This information will be useful in recording that this P-element *F insertion was the progenitor for the cmp44E alleles you created. *F 2. In addition, although this insertion does not cause a phenotype, I *F will create an allele of cmp44E for the insertion because it is *F inserted in the transcription unit (I will also record the information *F that the line does not cause a phenotype). *F 3. Also as this line originally had 2 P-element insertions, this will *F also be useful information to have for the line. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From jongens@mail.med.upenn.edu Wed Feb 10 22:18:07 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Feb 1999 22:18:07 +0000 *F Mime-Version: 1.0 *F Date: Wed, 10 Feb 1999 17:23:09 -0400 *F To: Gillian Millburn (Genetics) *F From: Tom Jongens *F Subject: Re: FlyBase query *F Dear Gillian, *F The line from which we obtained pngcl was k00114. We recombined *F away the lethality and the other P-element from the P-element insertion at *F 44E3,4(pngcl). If you have further questions don't hesitate to ask. *F Tom Jongens *F Thomas A. Jongens *F Dept. of Genetics *F University of Pennsylvania *F School of Medicine *F 709b Stellar-Chance Labs *F 422 Curie Blvd *F Philadelphia, Pa 19104-6100 *F tel:(215)573-9332 *F fax:(215)573-9411 # *U FBrf0107041 *a Oates *b A. *t 1999.2.2 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Feb 02 11:05:22 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 Feb 1999 11:05:22 +0000 *F To: aoates@molbio.princeton.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 2 Feb 1999 11:07:25 +0000 *F Content-Length: 987 *F Dear Dr. Oates, *F I am curating your paper for FlyBase: *F Oates et al., 1998, Mech. Dev. 78(1,2): 165--169 *F which is about the gene dnt (doughnut). *F there is already a gene in FlyBase with the name ''doughnut'' - it has *F the symbol ''dn'', is on the 3rd chromosome and is in Lindsley and Zimm. *F Would it be OK for the valid name of your gene dnt to be ''doughnut on *F 2'' as it is on the second chromosome, to distinguish it from the *F doughnut gene (dn) on chromosome 3. The symbol you used - ''dnt'' will *F stay as it is, as this symbol hasn't been used before. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From aoates@molbio.princeton.edu Tue Feb 02 16:04:36 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 Feb 1999 16:04:36 +0000 *F Mime-Version: 1.0 *F Date: Tue, 2 Feb 1999 11:19:17 -0500 *F To: Gillian Millburn (Genetics) *F From: Andrew Oates *F Subject: Re: FlyBase query *F Dear Gillian, *F yes, it is fine for the name to be changed to doughnut on 2. *F all the best, *F andy& *F Andy Oates *F Moffett Laboratories 323 *F Department of Molecular Biology *F Princeton University *F Washington Road *F Princeton *F New Jersey 08544 *F USA *F ph 609 258 3983 *F fax 609 258 1035 *F email aoates@molbio.princeton.edu # *U FBrf0107195 *a Russell *b S. *t 1999.1.25 *T personal communication to FlyBase *u *F From sr120@mole.bio.cam.ac.uk Mon Jan 25 18:40:46 1999 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 25 Jan 1999 18:40:46 +0000 *F Mime-Version: 1.0 *F Date: Mon, 25 Jan 1999 18:42:44 +0000 *F To: Rachel Drysdale (Genetics) *F From: Steve Russell *F Subject: Re: update framework *F Dear Rachel, *F Here are some data to update the entry for Dichaete Specifically, the *F statement in FBrf0075380; 'It is therefore unclear whether this lethality *F represents a vital function for the wild type allele of D itself as opposed *F to that of an adjacent gene.' is no longer correct. All of the lethal *F dominant D alleles are lethal over the EMS induced point allele Dr72 and *F the coding sequence deletion Dr513, indicating straightforward allelism. *F Furthermore, all of the lethal dominant alleles have been characterised *F with respect to the expression of Dichaete in the embryo, they all show *F tissue-specific loss of Dichaete expression and thus are regulatory *F mutations. The location of the breakpoints of each of the alleles is *F listed below, where 0 is the translational start of Dichaete. The range *F represents the uncertainty, thus D4 breaks in a restriction fragment *F between -9.8 and -12 and so on. *F T(2;3)D4 -9.8 to -12.4 *F In(3)D6 -6.5 to -9 *F T(2;3)D7 -16 to -18 *F In(3L)Dr8 -5.5 to -6.5 *F In(3L)D10 -21 to -24 *F There is some uncertainty in the designation of D1, D3 and D9 as dominant D *F alleles The wing phenotype of the dominant alleles is due to ectopic *F expression of Dichaete in the anlage of the hinge in the wing imaginal *F disc, the phenotype can be reproduced by driving UASDichaete in the wing *F hinge with the GAL4 drivers ms209 and 30A. both D3 and D9 are deletions of *F the Dichaete transcription unit, phenotypically they behave as null alleles *F when assayed in the embryo. *F D3 is an additional abberation on top of the D1 chromosome which results in *F the deletion of the 3' portion of the Dichaete transcription unit, it is a *F protein null when assayed in whole mount embryos with anti-D antisera. *F Since the wing phenotype of D3 is similar, if not identical to, D1 when *F assayed in outcrossed individuals, these data raise the possibility that D1 *F itself is not due to Dichaete. Dichaete protein is ectopically expressed *F in the wing discs of D1 individuals and reversion of D1 is associated with *F loss of Dichaete in the wing disc. It is possible that the D1 and D3 *F inversions reflect dominant mutations in Sail. *F D9 is a deletion which begins approximately 30 Kb 3' of the transcription *F unit and extends proximal, removing the transcription unit. The cause of *F the dominant phenotype in this case is not known. *F Steve # *U FBrf0107552 *a Schaefer *b U. *c H. *d Jackle *e Y. *f He *g H. *h Bellen *i T. *j Laverty *k G. *l Rubin *t 1999.3.29 *T personal communication to FlyBase *u *F Personal communication from: Ulrich Schaefer, Herbert Jackle, *F Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Dated: 29 March 1999 *F Background: The lethal alleles and P{lacW} insertions reported *F here were generated in the laboratory of Herbert Jackle *F (Goettingen) in a screen for lethal P{lacW} hops into the X *F chromosome. In situ hybridizations to map the P{lacW} insertions *F were carried out in the laboratory of Hugo Bellen (Houston). *F Hybridized chromosomes were read by both the Bellen lab and by *F Todd Laverty (Rubin lab, Berkeley). With the exception of *F ActnG0077, the assumption that lethality in single insertion *F lines is caused by the insertion has not been verified. lacZ *F expression patterns for these lines will be available from *F FlyView. *F *F Information communicated: *F *F Alleles (presumed to be caused by P{lacW} insertion) -- *F *F 'ActnG0077','2C1-2' *F 'l(1)G0007G0007','12E3-4' *F 'l(1)G0009G0009','5C5-8' *F 'l(1)G0018G0018','2B1-4' *F 'l(1)G0023G0023','3A1-4' *F 'l(1)G0028G0028','11B3-6' *F 'l(1)G0030G0030','5D3-6' *F 'l(1)G0035G0035','6F1-2' *F 'l(1)G0037G0037','1B7-8' *F 'l(1)G0039G0039','7E5-6' *F 'l(1)G0040G0040','8B1-4' *F 'l(1)G0043G0043','6F1-2' *F 'l(1)G0045G0045','5A5-6' *F 'l(1)G0050G0050','5D' *F 'l(1)G0051G0051','2B7-1' *F 'l(1)G0052G0052','1A3-5' *F 'l(1)G0054G0054','3F4-5' *F 'l(1)G0055G0055','3B1-2' *F 'l(1)G0056G0056','9E3-4' *F 'l(1)G0057G0057','13F1-2' *F 'l(1)G0058G0058','1C1-2' *F 'l(1)G0059G0059','6E4-5' *F 'l(1)G0061G0061','9E3-4' *F 'l(1)G0063G0063','5E1-2' *F 'l(1)G0066G0066','2C1-2' *F 'l(1)G0067G0067','8E3-4' *F 'l(1)G0071G0071','7E5-6' *F 'l(1)G0072G0072','9C3-4' *F 'l(1)G0074G0074','16D1-2' *F 'l(1)G0075G0075','9D' *F 'l(1)G0078G0078','? -- multi-insert line: 11D1-2, 17C1-4' *F 'l(1)G0079G0079','5C3-6' *F 'l(1)G0081G0081','4B1-2' *F 'l(1)G0083G0083','9F1-6' *F 'l(1)G0084G0084','18D5-8' *F 'l(1)G0095G0095','7F1-4' *F 'l(1)G0104G0104','12D' *F 'l(1)G0105G0105','1E3-4' *F 'l(1)G0108G0108','16C1-2' *F 'l(1)G0113G0113','? -- multi-insert line: 3F1-2, 7D1-2, 11A1-2' *F 'l(1)G0115G0115','1C2-3' *F 'l(1)G0117G0117','? -- multi-insert line: 5C1-2, 5D1-2' *F 'l(1)G0118G0118','6E1-2' *F 'l(1)G0119G0119','? -- multi-insert line: 8F4-5, 9A1-2' *F 'l(1)G0127G0127','9E1-2' *F 'l(1)G0129G0129','2B1-4' *F 'l(1)G0130G0130','2B1-2' *F 'l(1)G0132G0132','1D' *F 'l(1)G0136G0136','13E8-9' *F 'l(1)G0142G0142','1B' *F 'l(1)G0155G0155','7C' *F 'l(1)G0170G0170','2D1-1' *F 'l(1)G0178G0178','7F' *F *F Transposon insertions (from multi-insert lines) *F *F 'P{lacW}G0078a','11D1-2' *F 'P{lacW}G0078b','17C1-4' *F 'P{lacW}G0113a','3F1-2' *F 'P{lacW}G0113b','7D1-2' *F 'P{lacW}G0113c','11A1-2' *F 'P{lacW}G0117a','5C1-2' *F 'P{lacW}G0117b','5D1-2' *F 'P{lacW}G0119a','8F4-5' *F 'P{lacW}G0119b','9A1-2' # *U FBrf0107579 *a Ahmed *b Y. *t 1999.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:12:10 1999 *F To: yahmed@molbio.princeton.edu *F Subject: ADRC-374A *F Dear Yashi, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Function of Drosophila homologs of the APC tumor suppressor gene in *F the regulation of beta-catenin/Tcf.' *F You mention a gene that is new to FlyBase, Apc2 (we already have *F Apc:FBgn0015589 at 98F1-4 which I am presuming corresponds to your *F Apc1). *F Do you have a map location for Apc2? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From yahmed@molbio.princeton.edu Fri Mar 12 16:21:59 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-374A *F Dear Rachel, Sorry for the delay in getting back to you. Was on vacation... *F The map location for apc 2 is 95E. *F Regards, *F Yashi # *U FBrf0107581 *a Alphey *b L. *t 1993.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:03:57 1999 *F To: Luke.Alphey@zoo.ox.ac.uk *F Subject: ADRC-284A *F Dear Luke, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'A Drosophila homologue of the nuclear PP1 regulator NIPP-1' *F You mention a gene that is new to FlyBase, NiPp-1. *F Do you have a map location for NiPp-1? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From luke.alphey@zoology.oxford.ac.uk Wed Mar 03 11:53:48 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: ADRC-284A *F Dear Rachel, *F The fly homologue of NIPP-1 (Nuclear Inhibitor of Protein Phosphatase type 1) *F is at 53F, probably 53F4-5. We call it NIPP-1Dm, for obvious reasons, but if *F you (Flybase) are going to change the name, please let me know and I'll try *F to use the same name in our paper, which is currently in preparation. *F Best wishes, *F Luke *F \-- *F Luke Alphey is at the: *F Department of Zoology, University of Oxford *F South Parks Road, Oxford, OX1 3PS, UK *F Tel: +44 (0)1865-271157; Fax: +44 (0)1865-310447 *F E-mail: Luke.Alphey@zoo.ox.ac.uk # *U FBrf0107583 *a Andre *b L. *t 1999.4.14 *T personal communication to FlyBase *u *F >From lebivic@ibdm.univ-mrs.fr Wed Apr 14 18:18:18 1999 *F To: m.ashburner@gen.cam.ac.uk *F Subject: Bub3 *F Dear Dr. Ashburner *F you were right when you suggested to check our cytology. We have done it *F after reading the paper from Basu et al.and found the Bub3 gene mapping to *F 3R 99B3-5 in good agreement with this author. While our sequence and that *F of Basu are from the same gene we think that our sequence contains less *F sequencing errors since our peptide sequence is closer to other Bub3 genes *F than Basu's. This is obvious in the 167-189 region. The sequence you sent *F to me has something wrong since our reading farme goes all the way to the *F c-terminus QETKQK. I have to check the genebank entry again for mistakes in *F writing the sequence. *F Thanks for your comments. *F Yours sincerely *F Le Bivic Andre # *U FBrf0107584 *a Andrew *b D. *t 1999.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:40:27 1999 *F To: debbie_andrew@qmail.bs.jhu.edu *F Subject: ADRC-716A *F Dear Deborah, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Characterization of ET85D in the salivary gland and trachea.' *F You mention a gene that is new to FlyBase, characterized via an *F enhancer trap, ET85D. *F Do you have a gene symbol/name for the gene identified by the enhancer *F trap? Do you have a map location for the gene? It is nice if we can *F keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From dandrew@bs.jhmi.edu Wed Mar 03 14:19:26 1999 *F Subject: RE: ADRC-716A *F To: Rachel Drysdale *F Reply to: RE: ADRC-716A *F Dear Rachel, *F We are waiting to characterize the phenotype more fully before giving the *F gene a name. The enhancer insert maps to 85D13-14. We'll send a gene *F name as soon as we've settled on one. *F Thanks, *F Debbie *F From dandrew@bs.jhmi.edu Tue Mar 23 20:25:55 1999 *F Subject: gene name *F To: Rachel Drysdale *F Hi Rachel, *F We have decided on a gene name for the enhancer-line in region 85D (ET85D). We would like to call the gene pasilla, and abbreviate the gene name, ps. *F Thanks, *F Debbie # *U FBrf0107590 *a Arn *b E. *t 1999.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:54:58 1999 *F To: zoltany@stanford.edu *F Subject: ADRC-119 and 14W *F Dear Eric, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstracts: *F 'Characterization of protein complexes involved in bcd mRNA localization.' *F You mention a gene that is new to FlyBase, DEK, and say it is 'the *F Drosophila homolog of the DEK oncogene protein'. *F FlyBase already has a gene which is a homolog of human DEK, namely: *F \*a E(var)53E *F \*z FBgn0015217 *F \*b 2- *F \*c 53E *F \*j species == Homo sapiens; gene == DEK; OMIM:125264 *F \*x FBrf0083118 == Dorn et al., 1995, Europ. Dros. Res. Conf. 14: 326 *F \*E FBrf0083118 == Dorn et al., 1995, Europ. Dros. Res. Conf. 14: 326 *F \*e Enhancer of variegation 53E *F \*i E(var)2-53E *F \*p The @E(var)53E@ protein is related to the human DEK protein implicated *F \*p in acute myeloid leukemia. *F \*A E(var)53E1 *F \*z FBal0042525 *F \*x FBrf0083118 == Dorn et al., 1995, Europ. Dros. Res. Conf. 14: 326 *F \*E FBrf0083118 == Dorn et al., 1995, Europ. Dros. Res. Conf. 14: 326 *F \*i unnamed *F \*o P-element activity *F \*G FBti0012253 == P{hsneo.ry+}E(var)53E1 *F \*k Phenotypic class: enhancer | dominant { In(1)wm4h } *F \*k Phenotypic class: female sterile | recessive *F \*k Enhancer of PEV. Homozygotes are only semi-viable. Surviving females *F \*k are sterile. *F \*u Remobilisation of the P element reverts mutant phenotype. *F Is this the same gene as your 'dDEK'. *F If not, do you have a map location for DEK? It is nice if we can *F keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From zoltany@leland.stanford.edu Thu Mar 11 18:59:34 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-119 and 14W *F Hi Rachel, *F I'm sorry that I haven't gotten back to you yet, but the reason *F is that I have not yet been able to determine whether our DEK is the *F same as E(var)53E. The map position of our genomic sequence places it *F near the position reported by Dorn et al, but as we do not have *F their sequence we cannot be sure it is the same gene. I have emailed *F Dr. Reuter to request the sequence, but have yet to hear back. Do you *F have access to the abstract reporting E(var)53E? *F (FBrf0083118 == Dorn et al., 1995, Europ. Dros. Res. *F Conf. 14: 326) *F If you could forward the text of this abstract, it may contain *F information which would allow us to determine whether we are dealing *F with the same gene. I will be certain to let you know as soon as we *F have good evidence either way. *F best, *F Eric Arn *F From rd120@gen.cam.ac.uk Fri Mar 12 10:32:31 1999 *F To: zoltany@stanford.edu *F Subject: Re: ADRC-119 and 14W *F Dear Eric, *F I am faxing you a copy of FBrf0083118. It may be as well to keep the *F two records (E(var)53E and DEK) separate for now, but if you could give *F me the map position of your genomic sequence (which you indicated that *F you know) then that would be helpful. If you don't want to then that's *F fine too - just let me know, and we will be patient. We can always *F merge the E(var)53E and DEK records later, when things become clearer. *F Thanks for getting back to me, *F Rachel. *F From zoltany@leland.stanford.edu Fri Mar 12 18:39:03 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-119 and 14W *F Rachel, *F Our gene is placed in the Genome Project sequence at 53D. So, *F as before, I think it is likely to be the same as E(var)53E but I am *F awaiting a reply from Dr. Reuter to confirm this. Thanks for sending *F the fax - the abstract is interesting but unfortunately does not answer *F the identity question for us. Here's hoping you have a pleasant trip to *F the states. *F Best, *F Eric # *U FBrf0107592 *a Ashburner *b M. *t 1999.2.22 *T personal communication to FlyBase *u *F >From ma11@gen.cam.ac.uk Mon Feb 22 09:14:26 1999 *F To: ag24@gen.cam.ac.uk *F I have a copy of the draft Antp sequence paper and this lead to an *F exchange of email between Susan Celniker and I involving TK *F re his cc genes. On the basis of the attached we can rename *F cc1 .. cc7 and merge cc8 and Edg84A. *F \*a cc1 *F \> *F \*a Ccp84Aa *F \# *F \*a cc2 *F > *F \*a Ccp84Ab *F \# *F \*a cc3 *F \> *F \*a Ccp84Ac *F \# *F \*a cc4 *F \> *F \*a Ccp84Ad *F \# *F \*a cc5 *F \> *F \*a Ccp84Ae *F \# *F \*a cc6 *F \> *F \*a Ccp84Af *F \# *F \*a cc7 *F \> *F \*a Ccp84Ag *F \# *F \*a cc8 *F \+ *F \*a Edg84a *F \> *F \*a Edg84a *F \# *F >From ma11@gen.cam.ac.uk Thu Feb 18 18:47:29 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 18 Feb 1999 18:47:29 +0000 *F To: ma11@gen.cam.ac.uk, celniker@bdgp.lbl.gov *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Thu, 18 Feb 1999 18:49:45 +0000 *F Content-Length: 2907 *F I see that while these are mentioned in Kaufman et al., 1990, Adv. Genet. 27: 309--362 the note in FlyBase says that were un-named. Therefore *F I suggest that you do name these Ccp84A BUT NOT -1 to -7 but *F a .. g, ie Ccp84Aa .. Ccp84Ag, which is the standard way. *F Kaufman's paper describes 8 genes in this order: *F In direction of increasing cytology: cc1+ cc2+ cc3- cc4+ cc5+ cc6? cc7- cc8+ pb- zen2? *F where +/-/? means 5'-3', 3'-5' or unknown direction of transcription. *F You should reference this paper. Why you have 7 genes and TK had *F 8 I do not know (polymorphism ?). Perhaps you should discuss this with TK. *F >From celniker@currant.lbl.gov Thu Feb 18 19:33:25 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 18 Feb 1999 19:33:25 +0000 *F Mime-Version: 1.0 *F Content-Type: multipart/alternative; boundary='============_-1292755902==_ma============' *F Date: Thu, 18 Feb 1999 11:36:26 -0800 *F To: Michael Ashburner (Genetics) *F From: Susan Celniker *F Subject: *F Re: *F Content-Length: 11006 *F Dear Michael, *F We will use your suggested name with a-g. Thanks Sue *F >From kaufman@sunflower.bio.indiana.edu Fri Feb 19 13:03:08 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 19 Feb 1999 13:03:08 +0000 *F Mime-Version: 1.0 *F Date: Fri, 19 Feb 1999 08:05:31 -0500 *F To: Michael Ashburner (Genetics) *F From: kaufman@bio.indiana.edu (T. Kaufman) *F Subject: Re: cc genes *F Yes, they have the complete sequence and therefore have a much better *F picture than our individual clones. Especially since there is so much *F redundancy (sequence) among that set of transcripts. *F \--tk *F >Tom *F > *F >Kaufman et al., 1990, Adv. Genet. 27: 309--362 mention 8 cuticle *F >protein genes in the Antp cluster. Celniker et al have found 7 *F >which they would like to call Ccp84a..Ccp84g. Susan (who I *F >think will be sending you a preprint for comment) thinks *F >that the 8th is Edg84A, which would make sense. Sound OK to you ? *F > *F >Michael *F Thom Kaufman kaufman@sunflower.bio.indiana.edu *F \--- Biology Dept., Indiana University, Bloomington, IN 47405 --- *F 812-855-3033/Office--812-855-7674/Lab--812-855-2577/FAX # *U FBrf0107602 *a Baumgartner *b S. *t 1999.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:44:14 1999 *F To: Stefan.Baumgartner@medkem.lu.se *F Subject: ADRC-064 *F Dear Stefan, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'NIDOGEN:Sequence, expression and isolation of an ECM protein during *F Drosophila development.' *F You mention a gene that is new to FlyBase, Ndg. *F Do you have a map location for Ndg? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Stefan.Baumgartner@medkem.lu.se Wed Mar 10 09:03:12 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: ADRC-064 *F Dear Rachel, *F After discussions with the others, I can grant you the information below. *F ndg (nidogen) maps at 47A1,2, and the cDNA is hit by DS07871 (Dm4293). *F best wishes, and see you at the Conference *F Stefan *F \-- *F Stefan Baumgartner *F Lund University *F Dept. of Cell & Molecular Biology *F Section for Developmental Biology *F Box 94 *F 22100 Lund *F SWEDEN # *U FBrf0107615 *a Bloomington Drosophila Stock Center *b ?. *t 1999.2.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Feb 26 18:52:05 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: remRA74 *F Hi folks-- *F A little mistake that came from Lindsley and Zimm: *F rem1 was isolated as RA74, so a synonym should be remRA74. L & Z *F shortened the allele designation to remA instead of RA. *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0107616 *a Bloomington Drosophila Stock Center *b ?. *t 1999.3.2 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: MKRS and TM2 variants *F Date: 2 March 1999 *F *F Information communicated: *F *F MKRS, P{Delta2-3}99B *F TM2, ry[*] P{Delta2-3}99B # *U FBrf0107629 *a Brown *b N. *t 1999.2.14 *T personal communication to FlyBase *u *F >From ag24@gen.cam.ac.uk Sun Feb 14 16:26:20 1999 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Sun, 14 Feb 1999 16:26:20 +0000 *F To: nb117@mole.bio.cam.ac.uk, chle@uni-bayreuth.de *F Subject: Df(2R)59AD and moa *F Cc: ag24@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Aubrey de Grey *F Date: Sun, 14 Feb 1999 16:28:32 +0000 *F Content-Length: 454 *F Dear Nick and Christian, *F I am trying to sort out whether Df(2R)59AD does or does not delete moa. *F Nick reports that it ('sac') does; Christian reports that it ('l(2)59ABd') *F doesn't, but credits Nick for the info that 59ABd non-complements sac. *F (I speak of Genetics 150:791 and MGG 259:383, of course.) Could either *F of you comment on which of the usual range of possible explanations you *F favour? Many thanks in advance. *F Cheers, Aubrey de Grey *F FlyBase *F >From nb117@mole.bio.cam.ac.uk Sun Feb 14 18:54:34 1999 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Sun, 14 Feb 1999 18:54:34 +0000 *F Mime-Version: 1.0 *F Date: Sun, 14 Feb 1999 19:00:04 +0000 *F To: Aubrey de Grey , chle@uni-bayreuth.de *F From: Nick Brown *F Subject: Re: Df(2R)59AD and moa *F Dear Aubrey and Christian, *F My mistake, it should have been Df(2R)59AB that fails to complement moa/sac *F alleles, not Df(2R)59AD, which complements moa/sac alleles. Cheers, Nick *F Nick Brown *F Wellcome/CRC Institute *F Tennis Court Road *F Cambridge CB2 1QR *F England *F Tel 44-1223-334128 *F Fax 44-1223-334089 # *U FBrf0107630 *a Buff *b E. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:12:37 1999 *F To: buff@helix.mgh.harvard.edu *F Subject: ADRC-378B *F Dear Eugene, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Evidence for autonomous and non-autonomous mechanisms for the *F regulation of cell size by wolf and gargantua.' *F You mention two genes that are new to FlyBase, wlf and gar. *F Do you have map locations for wlf and gar? It is nice if we can keep as many *F gene records as possible anchored to the map. Do you have allele *F symbols for the mutant alleles, such as wlf1 and gar1? If so we can *F capture the data more rigorously than otherwise. *F Thank you for your help, *F with best wishes, *F Rachel. *F From buff@helix.mgh.harvard.edu Wed Mar 03 13:42:54 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-378B *F Dear Rachel, *F Unfortunately I do not have an exact location for the genes you mentioned. *F I know that they are on 2L arm (that's the one I screened) and I am trying *F to map them meiotically because they complement most of the deficiency kit *F for now. *F Eugene. *F __________________________________ *F Eugene Buff, MD, PhD *F MGH Cancer Center *F Bldg. 149, 13th Street *F Charlestown, MA 02129 *F ph.: (617) 724-9535 *F FAX: (617) 726-7808 *F buff@helix.mgh.harvard.edu *F __________________________________ # *U FBrf0107635 *a Camonis *b J. *t 1999.4.6 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Apr 01 15:50:32 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Apr 1999 15:50:32 +0100 *F To: Jacques.Camonis@curie.fr *F Subject: FlyBase query: ARDC40 - 860A *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 1 Apr 1999 15:53:12 +0100 *F Content-Length: 1135 *F Dear Dr. Camonis, *F I am curating the addendum book to the 40th Annual Drosophila Research *F Conference which was just held in Seattle. In your abstract: *F 'Searching the Ral GTPase function through two-hybrid and genetics.' *F you describe two genes which are new to FlyBase: *F 1. dRGL *F Do you have a map location for dRGL? It is nice if we can keep as many *F gene records as possible anchored to the map. *F 2. dRal *F As far as I know, dRal is new to FlyBase, although there is a 'Rala' *F (Ras-related protein) gene already in FlyBase. This is in the EMBL *F sequence database under accession number U23800. *F Is dRal the same as Rala ? If dRal is not the same as Rala, do you *F have a map location for dRal? *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Jacques.Camonis@curie.fr Tue Apr 06 16:44:01 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 6 Apr 1999 16:44:01 +0100 *F Mime-Version: 1.0 *F Date: Tue, 6 Apr 1999 16:41:36 +0100 *F To: Gillian Millburn (Genetics) *F From: Jacques Camonis *F Subject: Re: FlyBase query: ARDC40 - 860A *F Dear Gillian *F >1. dRGL *F This guy was located in 70C2-5, in a collaborative study with us by *F Christian Biemont, CNRS, Villeurbanne, France. *F >2. dRal *F > *F >As far as I know, dRal is new to FlyBase, although there is a 'Rala' *F >(Ras-related protein) gene already in FlyBase. This is in the EMBL *F >sequence database under accession number U23800. *F >Is dRal the same as Rala ? If dRal is not the same as Rala, do you *F >have a map location for dRal? *F No, you are right, this 'dRal' is what is called 'Rala' but it isn't clear *F that is more RalA than RalB. *F It is located at 3E, on the X *F There are two mistakes in the sequence that I found originally (2 years *F ago) in Genbank, that change two amino acids. But the sequence found in the *F EST section of BDGP is correct. *F Don't hesitate if I am not clear or if you want to know more. *F Jacques *F \------------------------------------------------------ *F Jacques H. Camonis, MD, PhD *F Directeur de Recherche CNRS *F Institut Curie, Section de Recherche, INSERM U248 *F 26 rue d'Ulm, 75248 Paris cedex 05 *F Tel.: 33-(0)142 34 66 54 *F Fax : 33-(0)142 34 66 50 *F E-mail : Jacques.Camonis@curie.fr *F http://www.curie.fr/ # *U FBrf0107637 *a Campbell *b G. *t 1999.3.29 *T personal communication to FlyBase *u *F From camp+@pitt.edu Mon Mar 29 15:59:37 1999 *F To: flybase-help@morgan.harvard.edu *F Subject: aristaless *F Hello, *F I am writing to correct a mistake in the file on the aristaless gene. *F The supposed al05142 allele is not an allele of aristaless, and is *F in fact a mutation in the dachsous gene. The P-element insert appears to *F map a long way from al - close to ds. The lacZ expression pattern of *F this line is superficially similar to al in the leg disc, but actually *F very closely resembles that reported for ds. *F Gerard Campbell *F Department of Biological Sciences *F University of Pittsburgh *F 215A Clapp Hall *F 4400 Fifth Avenue *F Pittsburgh, PA 15260 *F Tel: (412) 624 6812 *F Fax: (412) 624 4759 # *U FBrf0107638 *a Carpenter *b A. *t 1999.2.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 22 16:40:11 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(2L)pr-M1 *F Hi Folks-- *F We recently received a new deficiency stock from Adelaide Carpenter and a *F FB aberration entry needs to be created for it. *F The stock will be listed as *F 5084 Df(2L)pr-M1, dpov1 bw1/CyO *F in our next stock list update. *F All the following information is from Adelaide. *F Df(2L)pr-M1 *F Cytology: 038B03-06;040A03 *F X ray induced. Dominant, slightly rough eye phenotype associated with Df *F chromosome. Fails to complement l(2)k11226k11226, l(2)k09410k06410, *F l(2)k09410k09414 and l(2)0528705287. *F Thanks, *F Kevin *F ___________________________________________________________________________ *F From kcook@bio.indiana.edu Tue Feb 23 18:45:53 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: More on Df(2L)pr-M1 *F Cc: atc12@mole.bio.cam.ac.uk *F Hi folks-- *F Here is some additional complementation information for Df(2L)pr-M1 from *F Adelaide Carpenter. Df(2L)pr-M1 fails to complement the following mutations: *F 1. l(2)0705407054 *F The l(2)07054 locus is Acon according to Adelaide. *F 2. bur1, bur10523 and two other bur alleles *F 3. Two alleles of clumsy *F Mutations are viable, but cause uncoordination. The locus deficiency maps *F just proximal to bur. *F 4. pr1 *F Thanks, *F Kevin # *U FBrf0107653 *a Clark *b K. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:39:21 1999 *F To: K.A.Clark@m.cc.utah.edu *F Subject: ADRC-707A *F Dear Kathleen, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Mlp84B and D-PINCH are two LIM-domain proteins implicated in muscle *F cytoskeleton stability' *F You mention a gene that is new to FlyBase, dpin. We do not prefix gene *F symbols with d for drosophila, leaving pin, but pin is preoccupied *F (pin: pinto). How about pinch? It is short enough to be used as a *F symbol. *F Do you have a map location for 'dpin'? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From K.A.Clark@m.cc.utah.edu Wed Mar 03 18:29:51 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-707A *F Dear Rachel, *F The drosophila pinch gene is part of a gene family. Although there is no *F evidence now, there is likely to be one or two additional drosophila *F family members. So, if I could, I would like to use pin85A as the gene *F symbol for this gene. The sequence and some RNA localization studies are *F know published for this gene, and in the paper it was referred to as dpin *F and d-pinch.I think these should be referred to as alternate names in the *F final flybase description. The map location for the gene is 85A1-3, as *F determined by chromosome in situ hybridization. Right now this is *F unpublished, but I am planning to present the cytological location of the *F gene at the fly meeting, so feel free to include this information. *F Sincerely, *F Kathleen # *U FBrf0107660 *a Cohen *b S. *t 1999.4.9 *T personal communication to FlyBase *u *F Dear Marco *F *F FlyBase wants to curate the EMBL sequence records submitted by you - AJ010388 *F and DME010389: *F *F Could you please tell me what 'dttg' is ? *F and I do not understand why the Gupta et al reference is attached to the *F first of these records. *F *F Thankyou *F *F Michael Ashburner *F *F From Stephen.Cohen@embl-heidelberg.de Fri Apr 09 08:38:12 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 9 Apr 1999 08:38:12 +0100 *F Date: Fri, 9 Apr 1999 09:41:09 +0200 (MDT) *F X-Sender: cohen@popserver.embl-heidelberg.de *F Mime-Version: 1.0 *F To: Michael Ashburner (Genetics) *F From: Stephen.Cohen@EMBL-Heidelberg.de (Stephen Cohen) *F Subject: Re: Help FlyBase please *F *F Hi Michael *F *F We have no idea why it is called dttg. ttg was a name used for one of the *F vertebrate homologs long ago. the name has fallen out of use and been *F replaced by LMO. We think the gene should be called either dLMO (for *F clarity to the rest of the world) or Beadex (which you recall are gain of *F function alleles overexpressing the dLMO transcript). *F *F I have no idea why the gupta ref is appended. possibly they submitted the *F sequence of EP0443. we submitted 1306 and 1394 ac#'s AJ010388 and AJ010389. *F *F *F see you, *F *F steve *F [Gupta et al make no mention of Drosophila! I can find no mention of EP0443 *F in FlyBase - ma]. # *U FBrf0107661 *a Conley *b C. *t 1999.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:14:46 1999 *F To: caconley@macc.wisc.edu *F Subject: ADRC-403C *F Dear Catharine, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Identification of the Drosophila acyl-CoenzymeA oxidase gene.' *F You mention a gene that is new to FlyBase, acox. *F You indicate that acox maps to one of the BDGP P1 clones. Please could *F you tell us which one? It is nice if we can keep as many gene records *F as possible anchored to the physical map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From CACONLEY@macc.wisc.edu Fri Mar 05 19:35:57 1999 *F Subject: Correction on acox location *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F Please list the location of acox as: AC004434 = DS01261 (Phase I). *F Thank you. *F Catharine. # *U FBrf0107662 *a Bloomington Drosophila Stock Center *b ?. *t 1999.2.23 *T personal communication to FlyBase *u *F >From kcook@bio.indiana.edu Mon Feb 22 20:30:30 1999 *F Envelope-to: ag24@gen.cam.ac.uk *F Subject: Df(3L)BSC1 and Df(3L)BSC2 *F Personal communication from Kevin Cook, Bloomington Drosophila Stock *F Center, 2/22/99. *F The following describes two new deletions that were generated here at the *F Bloomington Stock Center. *F Males from the stock *F P2106 y* w*; P{w+mC=lacW}Phaj3D4/TM6B, AntpHu Tb1 *F were given 4000 rads from a cesium source, mated to females from the stock *F 3607 w1118; TM3, Sb1/CxD *F and white eyed progeny were recovered as potential chromosomal deletions. *F Two deletions, Df(3L)BSC1 and Df(3L)BSC2, were recovered. They have been *F incorporated into the Bloomington collection as stocks *F 5086 w1118; Df(3L)BSC1/TM3, Sb1 *F 5087 w1118; Df(3L)BSC2/TM3, Sb1 *F The deletions show the following complementation patterns: *F Df(3L)BSC1 complements *F kto1 (Stock 3618) *F Su(Tpl)10 (Stock 207) *F l(3)1191 (Stock P627) *F l(3)L3809L3809 (Stock P190) *F l(3)0167301673 (Stock P1543) *F l(3)L0090L0090 (Obtained from BDGP/Allan Spradling) *F Df(3R)rdgC-co2 (Stock 2052) *F and fails to complement *F Df(3R)kto2 (Stock 3617) *F l(3)L1243L1243 (Stock P196) *F Phaj3D4 (Stock P2106) *F Df(3L)BSC2 (Stock 5087) *F Df(3L)BSC2 complements *F l(3)1191 (Stock P627) *F l(3)L3809L3809 (Stock P190) *F l(3)0167301673 (Stock P1543) *F l(3)L0090L0090 (Obtained from BDGP/Allan Spradling) *F Df(3R)rdgC-co2 (Stock 2052) *F and fails to complement *F kto1 (Stock 3618) *F Df(3R)kto2 (Stock 3617) *F Su(Tpl)10 (Stock 207) *F l(3)L1243L1243 (Stock P196) *F Phaj3D4 (Stock P2106) *F Df(3L)BSC1 (Stock 5086) *F In polytene chromosome squashes, the distal breakpoints were easily *F determined, but proximal breakpoints are in a region of poor banding. The *F following are my best estimations of breakpoint cytology: *F Df(3L)BSC1 076D02-03:076D05-08 *F Df(3L)BSC2 076C;076F02-03 *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0107665 *a Coyne *b J. *t 1998.5.14 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Jerry Coyne, University of Chicago *F To: Bloomington Drosophila Stock Center *F Subject: 7-11HD *F Dated: 14 May 1998 *F *F Information communicated: *F *F Gene symbol 7-11HD *F Full Name 7,11-heptacosadiene modifier *F Map position between cu and kar *F Allele symbol 7-11HD[1] *F Phenotype changes the proportion of 7,11-heptacosadiene:5,9-heptacosadiene from high to low *F Origin collected in the Dominican Republic in 1993. The strain carrying this homozygous wild-caught 3rd chromosome, which also carries In(3L)P, is also known as Jaragua 3B. # *U FBrf0107670 *a Dearolf *b C. *t 1999.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:20:31 1999 *F To: cdearolf@partners.org *F Subject: ADRC-487C *F Dear Charles, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Cloning and expression of D-eIF4c and its relation to the Tum-l *F leukemia model.' *F You mention a gene that is new to FlyBase, eIF-4c. *F Do you have a map location for eIF-4c? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From CDEAROLF@partners.org Fri Mar 05 15:22:25 1999 *F To: 'Rachel Drysdale' *F Subject: RE: ADRC-487C *F Rachel, we have mapped the gene to 90E-91A, as determined by the *F recovery of multiple clones from P1 grids. Regards, Chuck Dearolf # *U FBrf0107677 *a Dingwall *b A. *t 1999.3.15 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:45:52 1999 *F To: akdingwa@mailbox.syr.edu *F Subject: ADRC-826C *F Dear Andrew, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Molecular genetic analysis of dENL/AF9: A gene involved in cell fate *F control and disease.' *F You mention a gene that is new to FlyBase, ENL/AF9. *F Do you have a map location for ENL/AF9? It is nice if we can keep as *F many gene records as possible anchored to the map. Were you intending *F to stick with ENL/AF9 as the gene symbol and name (we must drop the 'd' *F \- we do not prefix with d for Drosophila in FlyBase)? Or perhaps you *F have by now discovered that ENL/AF9 corresponds to a previously defined *F locus. *F Thank you for your help, *F with best wishes, *F Rachel. *F From akdingwa@mailbox.syr.edu Mon Mar 15 15:38:45 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-826C *F Hi Rachel: *F Our 'new' gene, dENL/AF9 is a homolog of two human genes named ENL and AF9. *F These names are essentially meaningless out of their context in humans *F (they have no relation to function or phenotypes), and we have not yet *F assigned a new name until we make more progress on the genetics. We have *F roughly mapped the gene to 88E-F, but without more molecular and genetic *F details we cannot assign a gene order in that region, so we have been *F hesitant to make any such assignments. *F I am not sure how you want to treat this information; we have been *F reluctant to re-name as we don't want to create any unnecessary confusion *F if it turns out to be a known locus. I hope this helps in some way. *F Regards, *F Andrew Dingwall # *U FBrf0107679 *a Doane *b W. *t 1999.3.16 *T personal communication to FlyBase *u *F From wdoane@asu.edu Tue Mar 16 19:50:34 1999 *F Subject: New name for an old gene *F To: flybase-help@morgan.harvard.edu *F I need some advice from the consortium of FlyBase on renaming the *F fs(2)lto5 gene locus, as well as the adpfs allele which was originally *F described as a mutant allele of the adipose gene. A paper is being *F prepared about this and before submitting it for publication I would *F like to have some general agreement on the names I am proposing. *F In 1960 I described a female sterility mutant that causes maternal *F effect lethality that generally ends in metaphase arrest during mitotic *F cycles 5 or 6, or earlier, in the preblastoderm embryo. I named it *F female sterile(2)adipose (fs(2)adp) because of the hypertrophied fat *F bodies associated with mutant homozygotes, which express twice the *F normal lipid content as wild-type flies. I subsequently isolated and *F described a fertile 'allele' of the original mutant and, on these *F grounds, the locus was renamed adipose (adp) by Lindsley and Grell *F (1968). Accordingly, the original female sterility mutant was dubbed *F adpfs and the fertile allele adp60 after the year in which it was *F isolated. *F Recently I reported in DIS that the adpfs mutation fails to compliment *F fs(2)lto5, one of the EMS-induced mutants isolated by Nusslein-Volhard *F and Weischaus in their saturation screen for female-sterile loci on *F chromosome 2. Now I have genetic recombination data *F that conclusively show that the female sterility gene locus in question *F is not the same gene *F as adp; indeed, it appears to be located about 1 cM distal to adp. *F I therefore propose that the fs(2)lto5 locus be renamed 'maternal *F metaphase arrest' with *F mama as its genetic symbol. Accordingly, my original fs(2)adp mutant *F allele (syn., adpfs) should be renamed mama1, because it was the *F first allele at this locus to *F be described, and the EMS-induced allele might be renamed mamalto5. *F Since I was *F the first to describe the mutant phenotype for this locus, I believe I *F am entitled to rename *F it...at least that is what Trudi Schubach told me. My papers describing *F various aspects of the mama mutant phenotype predate by more that 25 *F years the sparse description of *F fs(2)lto5 in FlyBase and in the Lindsley and Zimm's compilation. *F I propose that the name for the adipose gene be retained since this was *F the first 'obesity *F gene' described in Drosophila and this name fits the mutant phenotype. *F The fertile *F adp60 mutant allele is probably the same naturally occurring mutation *F that was linked to the above mama1 because both came from the Kaduna *F wild population maintained at Edinburgh. However, one can't be sure *F they are identical so I hesitate to suggest that adp60 be renamed *F adp1, which would be appropriate for the adp mutation originally *F linked to mama1. *F Please let me know if you think the new names I proposed above are *F suitable, as well as the genetic symbols for mama and adp mutant *F alleles. Also, if you have any suggestions about what journal would be *F most suitable for the paper I am preparing, I would welcome them. *F With many thanks, *F Winifred Doane *F Wdoane@asu.edu # *U FBrf0107680 *a Doane *b W. *t 1999.3.19 *T personal communication to FlyBase *u *F From wdoane@asu.edu Fri Mar 19 17:06:53 1999 *F Subject: Re: New name for an old gene *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F . *F . *F I don't think I *F mentioned that the adp locus is about 0.6 cM distal to stau and Pcl, so the *F gene order is stau-Pcl-pAbp-adp-mama. *F Best regards, *F Winifred # *U FBrf0107682 *a Dorsett *b D. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:16:52 1999 *F To: d-dorsett@ski.mskcc.org *F Subject: ADRC-434A *F Dear Dale, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Nipped-B, a Drosophila Homologue of Chromosomal Adherins, Participates *F in Activation by Remote Enhancers in the cut and Ultrabithorax Genes.' *F You mention two genes that are new to FlyBase, Nipped-A and Nipped-B. *F Do you have map locations for Nipped-A and Nipped-B? It is nice if we *F can keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From d-dorsett@ski.mskcc.org Wed Mar 03 15:05:47 1999 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 3 Mar 1999 15:05:47 +0000 *F X-Sender: ddorsett@pop.ski.mskcc.org *F Mime-Version: 1.0 *F Date: Wed, 3 Mar 1999 10:05:08 -0500 *F To: Rachel Drysdale (Genetics) *F From: d-dorsett@ski.mskcc.org (Dale DORSETT) *F Subject: Re: ADRC-434A *F Hi Rachel, *F Nipped-A and Nipped-B are in 41A, between l(2)41Af and straw. Nipped-A is *F allelic to l(2)41Ah, but Nipped-B has not been described before. *F Cheers, *F \-Dale *F \---------------------------------------------------------------------- *F Dale Dorsett *F Molecular Biology Program *F Box 73 *F Memorial Sloan-Kettering Cancer Center TEL 212 639 8498 *F 1275 York Ave FAX 212 717-3623 *F New York, NY 10021 d-dorsett@ski.mskcc.org *F \---------------------------------------------------------------------- # *U FBrf0107687 *a Dunkov *b B.C. *c J.H. *d Law *t 1999.3.20 *T personal communication to FlyBase *u *F >From dunkov@u.arizona.edu Sat Mar 20 01:08:29 1999 *F Envelope-to: m.ashburner@gen.cam.ac.uk *F Delivery-date: Sat, 20 Mar 1999 01:08:29 +0000 *F X-Sender: dunkov@pop.u.arizona.edu *F Mime-Version: 1.0 *F Date: Fri, 19 Mar 1999 18:20:25 -0700 *F To: m.ashburner@gen.cam.ac.uk *F From: Boris Dunkov *F Subject: Ferritin genes in FlyBase *F Cc: spradling@mail1.ciwemb.edu *F Dear Michael, *F Our interest in Drosophila iron metabolism resulted in description of two *F genes encoding ferritin subunits a couple of years ago. Unfortunately, then *F we did not know much about these subunits and consequently we helped create *F two entries in FlyBase that are not accurate any more. Here is a proposal *F for changes that in our view would improve the current situation and will *F prevent further perpetuation of our old confusion. *F Proposed changes in the nomenclature of the Drosophila ferritin genes *F Ferritin is the principal biological iron storage protein. Vertebrate *F ferritins are large spherical molecules that are composed of 24 subunits, *F generally of two types, designated H (heavy) and L (light), based upon *F rather small differences in the size of human subunits. Unfortunately, *F these differences in size are reversed in some animals (e. g. in Drosophila *F and in other insects), even among the vertebrates, but the terminology, now *F based upon sequence similarities, has been retained. Additional subunits *F are present in some animals. H and L chains are products of separate, but *F related, genes. Insects that have been studied so far also have two types *F of subunits, encoded by different genes. D. melanogaster ferritin is *F composed of three major subunits with apparent molecular masses of 25, 26, *F and 28 kDa that are products of two distinct genes (Charlesworth et al., *F Eur. J. Biochem., 247:470-475, 1997). The 25-kDa and 26-kDa subunits are *F derived from the same gene. Based on the apparent molecular masses of the *F subunits, we initially proposed a nomenclature for these genes which was *F adopted by FlyBase: Fer-H, for the 'heavy' 28-kDa chain, and Fer-L, for the *F 'light' 26-kDa and 25-kDa chains. When the complete sequences of both *F subunit types from D. melanogaster were obtained, it became clear that the *F sequence of the 'light' chain (FER-L, product of gene Fer-L) is more *F similar to the vertebrate H chains and that of the 'heavy' chain (FER-H, *F product of gene Fer-H) is closer to the vertebrate L chains. In order to *F avoid further confusion and at the same time to conform to the widely *F accepted terminology for vertebrate ferritins we adopted a more neutral *F designation of the Drosophila ferritin subunits and the corresponding *F genes. This is reflected in the GenBank entry for the first Drosophila *F ferritin sequence, the one for the 25-kDa and 26-kDa subunits, with *F accession number U91524. In this entry we designate the sequence as coding *F for the 'ferritin subunit 1', the protein as 'FER1', and the gene as *F 'Fer1'. This was adopted by other investigators and used in their GenBank *F entries with accession numbers Y15629, Y15630, Y15631, Y15632, and Y15633. *F The sequence of the 28-kDa subunit, derived from ESTs obtained by BDGP, we *F designate as 'ferritin subunit 2', the protein as 'FER2', and the gene as *F 'Fer2'. In these neutral names for the subunits of Drosophila ferritin (and *F similarly for the ferritin subunits of at least two other insect species) *F the important fact that they resemble the vertebrate H and L chains *F respectively is not evident. This may leave the impression that Drosophila *F ferritins are composed of two types of subunits but with sequences more *F similar to vertebrate H chains than to vertebrate L chains, as it is the *F case in the trematode worm Schistosoma mansoni (Dietzel et al., Molec. *F Biochem. Parasitol., 50:245-254, 1992) and in the nematode Caenorhabditis *F elegans (unpublished observation). Now we know that not only Drosophila, *F but other insects as well, have ferritins composed of at least two types of *F subunits homologous to vertebrate H and L chains. To indicate this *F important finding we propose the neutral names for the Drosophila subunits *F to be amended with the descriptions 'heavy chain homologue' and 'light *F chain homologue' abbreviated as 'HCH' and 'LCH' respectively. We have *F already used the name 'ferritin heavy chain homologue' and the *F abbreviations 'FER1 HCH' and 'Fer1 HCH' for the 'ferritin subunit 1' in a *F recent publication (Georgieva et al., Proc. Natl. Acad. Sci. USA, *F 96:2716-2721, 1999). We retain the number '1' in the name of the subunit *F for two reasons: (1) additional HCH or LCH subunits may be found and *F eventually given different numbers and (2) the name 'ferritin subunit 1' *F from our original GenBank entry was adopted and used by others, including *F in a number of EST entries generated by BDGP. *F We propose the following changes in the FlyBase entries for the ferritin genes: *F Current New *F _______________________________________________________________________________ *F Gene Fer-L Gene Fer1HCH *F Symbol Fer-L Fer1HCH *F Full name Ferritin-Light Ferritin 1 heavy chain *F homologue *F Function of product ferritin light chain ferritin heavy chain homologue *F Cytological location 99F6--11 99F1-2 *F _______________________________________________________________________________ *F Gene Fer-H Gene Fer2LCH *F Symbol Fer-H Fer2LCH *F Full name Ferritin-Heavy Ferritin 2 light chain *F homologue *F Function of product ferritin heavy chain ferritin light chain homologue *F Cytological location - 99F1-2 *F We believe that the proposed changes fully reflect the current knowledge *F on Drosophila and other insect ferritins. In our view the implementation *F and dissemination of the new names of the ferritin genes trough FlyBase *F will prevent further use of the old inaccurate names that were a result of *F our own confusion. We hope that FlyBase will find our arguments solid *F enough and will accept the proposed changes. *F Sincerely, *F Boris C. Dunkov John H. Law *F Department of Biochemistry Department of Biochemistry *F University of Arizona University of Arizona *F BSW 351 BSW 345 *F Tucson, AZ 85721 Tucson, AZ 85721 *F Tel: (520)621-3046 Tel: (520)621-8318 *F Fax: (520)621-9288 Fax: (520)621-3243 *F e-mail: dunkov@u.arizona.edu e-mail: jhlaw@u.arizona.edu # *U FBrf0107690 *a Edwards *b K. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:06:14 1999 *F To: kedwards@hawaii.edu *F Subject: ADRC-321B *F Dear Kevin, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Rhophilin, a putative effector for the cytoskeletal regulator rho, is *F not essential for development.' *F You mention a gene that is new to FlyBase, Rhp. *F There is another *F abstract concerning rhophilin: *F Program Number: 318B *F Signaling mechanisms controlling cell shape during gastrulation *F U. Haecker, U. Schaefer, H. Jaeckle and N.Perrimon. *F I imagine this is the same gene as yours. Do you know whether this is *F the case? If they are the same I need only create one new gene entry *F in FlyBase but if they are distinct (and the information concerning mutant *F phenotypes seems rather different) then I must create two new records. *F You mention that your rhophilin corresponds to a Berkeley EST, could *F you tell me which one? This is information that we like to track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kedwards@hawaii.edu Wed Mar 03 21:11:53 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-321B *F Dear Rachel, *F I just called Udo Hacker and he is withdrawing the work, believing *F his lethal *F phenotype is not due to rhophilin. We haved sequenced the same clone, *F LD12055. I just deposited the insert of this clone to genbank, acc# *F AF132025, soon to be *F released. Hacker confirms my cytology as 13EF. I believe it's on the 13E *F side since it is not removed by Df(1)sd<72b26> based on in situ. *F Thanks, *F Kevin Edwards *F =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= *F kedwards@hawaii.edu Yamamoto Behavior Genes Project *F fax 808-956-2440 CCRT, University of Hawaii *F lab 808-956-4909 Honolulu HI 96822 # *U FBrf0107694 *a Eizov *b M. *t 1999.4.10 *T personal communication to FlyBase *u *F From ems@uic.nnov.ru Sat Apr 10 14:54:15 1999 *F To: flybase-help@morgan.harvard.edu *F Subject: addition to the FlyBase report *F Dear Rachel, *F might I made an addition to the FlyBase report about *F leg-arista-wing-complex (lawc) gene ? As lawc and l(1)EF520 are allelic *F (see below), *F > The complementation test showed that there was no heteroallelic >complementation between EMS-induced embrionic lethal l(1)EF520 and lawcp1. *F > (Simonova et al., A. Dros. Res. Conf. 39: 333A) *F lawc localization should be changed from 7F1 to 7E4 cytological map *F position (Perrimon et al. 1989, Genetics, 121: 333-352). *F Besides, I would like to notes that phenotype of some mutations, which *F are at the same position of the genetic map, are resemble lawc *F phenotype, excluding homeotic transformation arista to tarsus. *F This mutations had been obtained by Neel and Fahmy (DIS-33: 81-94) and *F were lost now. Do compare: *F > Symbol scr1 *F > Discoverer Neel, 22nd Feb. 1941. *F > FlyBase ID FBal0015276 *F > Genetic location 1-22.0 *F > Phenotypic info. *F > *F > Hairs and bristles missing or doubled and deranged. Eyes small and *F > rough. Scutellum more convex than wild type. Wing margins, especially *F > posterior, often incised. Wings occasionally blistered. All characters *F > variable; a few flies appear normal. RK3. *F \----------------------------------------------------------------------- *F > Symbol lawc1 *F > FlyBase ID FBgn0005688 *F > Genetic location 1-23.0 *F > Phenotypic info. *F > *F > Wings have a ct phenotype, are wide apart and slightly *F > raised. Additional bristles are present on the head, *F > thorax and scutellum. Rough eye phenotype. *F > Transformation of arista in tarsus and distortion of distal *F > leg regions. *F \----------------------------------------------------------------------- *F > Symbol bwl (bow-legged) *F > FlyBase ID: no matches for querry *F > Genetic location 1-21.9+-0.6 *F > Phenotypic info. *F > *F > Inviable fly with shorter, divergent wings. Macrohaeta slightly tinner and > shorter. Legs shorter and one or both of yhe lohg joints (femur and/or tibia > bow-shaped. Males sterile. *F \----------------------------------------------------------------------- *F Best wishes, *F Max S. Eizov *F Graduate student *F Nizhnii Novgorod University *F Biology Faculty *F ems@uic.nnov.ru # *U FBrf0107696 *a Engels *b W. *t 1999.4.8 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Apr 07 17:54:14 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Apr 1999 17:54:14 +0100 *F To: wrengels@facstaff.wisc.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 7 Apr 1999 17:57:08 +0100 *F Content-Length: 1116 *F Dear Dr. Engels, *F I am curating your paper for FlyBase: *F Kusano et al., 1999, Genetics 151(3): 1027--1039 *F which is about Dmblm. *F I would be grateful if you could confirm that Dmblm is the same gene as *F the gene currently in FlyBase as 'gesta1'. *F This gene was mentioned as a RecQ homologue in Kusano et al., 1996, A. *F Conf. Dros. Res. 37: 152, and was called 'gesta1' in Kusano et al., *F 1997, A. Conf. Dros. Res. 38: 106C. *F If the 2 genes are the same, could you confirm that you wish the valid *F symbol and name of the gene to be 'blm' (we will remove the 'Dm' from *F the symbol as it stands for D. melanogaster) and 'bloom' respectively, *F so that I can change the entry in FlyBase. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From wrengels@facstaff.wisc.edu Thu Apr 08 20:32:04 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Apr 1999 20:32:04 +0100 *F Mime-Version: 1.0 *F Date: Thu, 8 Apr 1999 14:34:59 -0500 *F To: Gillian Millburn (Genetics) *F From: Bill Engels *F Subject: Re: FlyBase query *F Yes, Dmblm is the same gene as gesta1. We renamed it on the advice of the *F reviewers. *F -- Bill Engels *F \------------------------------------------------------------------------------- *F >Dear Dr. Engels, *F > *F >I am curating your paper for FlyBase: *F > *F >Kusano et al., 1999, Genetics 151(3): 1027--1039 *F > *F >which is about Dmblm. *F > *F >I would be grateful if you could confirm that Dmblm is the same gene as *F >the gene currently in FlyBase as 'gesta1'. *F > *F >This gene was mentioned as a RecQ homologue in Kusano et al., 1996, A. *F >Conf. Dros. Res. 37: 152, and was called 'gesta1' in Kusano et al., *F >1997, A. Conf. Dros. Res. 38: 106C. *F > *F >If the 2 genes are the same, could you confirm that you wish the valid *F >symbol and name of the gene to be 'blm' (we will remove the 'Dm' from *F >the symbol as it stands for D. melanogaster) and 'bloom' respectively, *F >so that I can change the entry in FlyBase. *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- # *U FBrf0107711 *a Ferrus *b A. *t 1999.3.4 *T personal communication to FlyBase *u *F Archived. # *U FBrf0107716 *a Flores *b C. *t 1999.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:32:37 1999 *F To: ccflores@facstaff.wisc.edu *F Subject: ADRC-652C *F Dear Carlos, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Drosophila DNA repair genes mre11 and nbs.' *F You mention a gene that is new to FlyBase, nbs. *F Do you have a map location for nbs? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From ccflores@facstaff.wisc.edu Thu Mar 11 21:29:56 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: nbs *F Dear Rachel, *F The nbs gene lies in region 67C10-D1. *F Sincerely, *F Carlos Flores # *U FBrf0107721 *a Galloni *b M. *t 1999.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:55:50 1999 *F To: mgalloni@fred.fhcrc.org *F Subject: ADRC-127 *F Dear Mireille, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Growth defective mutants of Drosophila melanogaster: insights into *F organismal growth regulation.' *F You mention two genes that are new to FlyBase, milou and bonsai. *F Do you have map locations for milou and bonsai? It is nice if we can *F keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mgalloni@fred.fhcrc.org Thu Mar 04 02:46:06 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-127 *F Dear Rachel, *F milou has been mapped at 60D1-D10 and bonsai at 58F. Please let me *F know if there is anything else I can provide you. *F Best wishes, *F Mireille Galloni. # *U FBrf0107726 *a Gassman *b A. *t 1999.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:08:45 1999 *F To: bsaxton@bio.indiana.edu *F Subject: ADRC-340C *F Dear William, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Identifying components of kinesin-based fast axonal transport.' *F You mention a gene that is new to FlyBase, EK4. *F Do you have a map location for EK4? It is nice if we can keep as many *F gene records as possible anchored to the map. If you have settled on a *F more informative gene symbol/name we could take this opportunity to *F record it in FlyBase..... *F Thank you for your help, *F with best wishes, *F Rachel. *F From agassman@sunflower.bio.indiana.edu Thu Mar 11 18:54:24 1999 *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F I writing you in response to your inquiry about the name and location of *F our mutation. In the lab it is called eek4 which is in flybase as *F E(Khc)ek4 and has a synonym Ek4. Its genetic location is 3-0.0. And this *F is the mutant allele so there are two alleles, the wild type and the *F mutant. *F Cheers Andrew # *U FBrf0107729 *a Gelbart *b W.M. *t 1999.2.26 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Feb 24 14:34:41 1999 *F To: gelbart@morgan.harvard.edu *F Subject: chromosome question *F Hi Bill, *F I am doing some work on the In(2L)dppd36 record - inspired by an *F update from John Roote. *F We currently have Dp(2;2)DTD48 as a segregant from Tp(2;2)DTD48. *F The record for Tp(2;2)DTD48 includes no mention of In(2L)dppd36. *F The record for In(2L)dppd36 includes no mention of Tp(2;2)DTD48. *F Your pc (FBrf0105410) mentions not In(2L)dppd36. *F The thing is that *F FBrf0098381 == The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Russia, 1997, D. I. S. 80: 109--130 *F mentions (page 118 stock number 2049) a chromosome that is basically *F both In(2L)dppd36 and Dp(2;2)DTD48. This matches with what you sent *F to John 'In(2L)dppd36, Dp(2;2)DTD48/In(2LR)Gla ' (in terms of its *F aberrations). *F My question is, was the DTD48 duplication induced on the inversion, or *F vice versa? *F Many thanks, *F Rachel. *F From gelbart@morgan.harvard.edu Fri Feb 26 22:22:41 1999 *F To: rd120@gen.cam.ac.uk *F Subject: Re: chromosome question *F Hi Rachel, *F The origin of Tp(2;2)DTD48 completely predates In(2L)dppd36. In(2L)dppd36 *F was induced on a genetic background of dpp+ Sp1 Bl1 Dp(2;2)DTD48 by *F Vivian Irish (her dissertation, 1986; Irish and Gelbart (Genes Dev. 1987 1:868--879). *F In(2L)dppd36 is a class V disk allele that is completely separable from *F the Dp(2;2)DTD48 by recombination. *F Bill # *U FBrf0107743 *a Green *b M. *t 1999.2.6 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Feb 10 21:47:49 1999 *F To: rd120@gen.cam.ac.uk *F Subject: bae *F Hi Rachel-- *F We recently (2/6/99) received from Mel Green a stock of a mutation he calls *F batone (bae). *F Here is what his note said: *F 'Enclosed is a copy of batone (bae). X chromosome at 54 +/-, just to the *F left of v. Maps within deficiency 14F6-15A1. Spontaneous on 68d22. Wings *F uninflated. Males and females viable and fertile. Non-autonomous in *F gynanders and fate maps to the head. I guess this is a brain mutant. The *F stock is y w bae/FM6.' *F No description of the mutation has been published. *F Thanks, *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0107744 *a Griffith *b L. *t 1999.2.26 *T personal communication to FlyBase *u *F From nobody@morgan.harvard.edu Fri Feb 26 14:55:44 1999 *F From: griffith@brandeis.edu (Leslie Griffith) *F Subject: FlyBase Help Mail *F To: flybase-help@morgan.harvard.edu *F griffith@brandeis.edu (Leslie Griffith) sent the following *F comments to flybase-help: *F \------------------------------------------------------------ *F I was browsing CaMKII and noticed that there is a link to Adf1 that was generated by an abstract we presented several years ago that a P element in Adf1 was an enhancer of CaMKII. We have subsequently found that the enhancer activity does NOT map to the P element- there was an additional unmarked mutation on the chromosome. Bottom line: Adf1 is NOT an enhancer of CaMKII. This should be corrected...Thanks- Leslie *F \------------------------------------------------------------ # *U FBrf0107751 *a Hales *b K. *t 1999.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:04:24 1999 *F To: peifer@unc.edu *F Subject: ADRC-292C and 3W *F Dear Mark, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Immunolocalization, structural studies, and genetic analysis of the Sep2 septin.' *F and *F 'The septin proteins of Drosophila: their roles in cytokinesis and other processes.' *F You mention two genes that are new to FlyBase, Sep4 and Sep5. *F Do you have map locations for Sep4 and Sep5? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kghales@email.unc.edu Fri Mar 12 19:49:30 1999 *F To: rd120@gen.cam.ac.uk *F Subject: Sep4 and Sep5 *F Dear Rachel, *F Mark Peifer asked me to respond to your inquiry regarding Sep4 and Sep5 *F (abstracts 292C and 3W). Sep4 has been characterized by Nick Brown and *F colleagues and is in 15A1-2. You should confirm that with Nick and cite *F him for the information. *F I have characterized Sep5 and found by P1 hybridization and P element *F insertion analysis that it is in 43F. *F Regards, *F Karen # *U FBrf0107761 *a Hayden *b A. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:05:09 1999 *F To: a.hayden@ucl.ac.uk *F Subject: ADRC-311A *F Dear Anne, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Investigating a possible role for the wunen homolog, DrPAP2G, in germ *F cell migration and axon guidance.' *F You mention a gene that is new to FlyBase, PAP2G. *F Do you have a map location for PAP2G? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From a.hayden@ucl.ac.uk Wed Mar 03 12:11:40 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: ADRC-311A *F Dear Rachel, *F We do not have in situ data for chromosome localisation but PCR analysis of *F genomic clones from the wunen contig suggests it lies next to wunen in 45D. *F We are currently sequencing the appropriate genomic clone to verify this. *F Anne # *U FBrf0107768 *a Hiromi *b Y. *t 1999.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:15:56 1999 *F To: yhiromi@lab.nig.ac.jp *F Subject: ADRC-417B *F Dear Yasushi, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Mutant analysis of a transcriptional coactivator MBF1' *F You mention a gene that is new to FlyBase, Mbf1. *F Do you have a map location for Mbf1? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From yhiromi@lab.nig.ac.jp Thu Mar 11 23:08:00 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-417B *F Cc: jmarek@lab.nig.ac.jp *F Dear Rachel, *F The mbf1 gene maps to 73A. Marek has the information on its location on the physical map, and can provide it to you if needed. *F FYI, Marek is a postdoc with Susumu HIROSE, who is the senior author on this poster. The Hirose lab had identified MBF1 as a factor from silkmoth that is required for the in vitro transcription of ftz. Marek has cloned the Drosophila homolog, and I'm helping him on the genetic analysis of mbf1. *F With best wishes, *F Yash Hiromi # *U FBrf0107779 *a Hyde *b D. *t 1999.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:29:08 1999 *F To: david.r.hyde.1@nd.edu *F Subject: ADRC-570B *F Dear David, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Retinal degeneration G (rdgG): a novel retinal degeneration mutation *F in Drosophila melanogaster.' *F You mention a gene that is new to FlyBase, rdgG. *F Do you have a map location for rdgG? It is nice if we can keep as many *F gene records as possible anchored to the map. Do you have an allele *F symbol for your rdgG mutant? *F Thank you for your help, *F with best wishes, *F Rachel. *F From hyde.1@nd.edu Fri Mar 12 22:57:35 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-570B *F Rachel, *F Our current data places rdgG most likely between map units 58.6-61.5 on the *F second chromosome. Using available deficiencies, rdgG most likely maps *F 45D-46A. We have designated the first allele as rdgG1. I hope that this *F helps. *F David # *U FBrf0107797 *a Jindra *b M. *t 1999.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:15:56 1999 *F To: yhiromi@lab.nig.ac.jp *F Subject: ADRC-417B *F Dear Yasushi, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Mutant analysis of a transcriptional coactivator MBF1' *F You mention a gene that is new to FlyBase, Mbf1. *F Do you have a map location for Mbf1? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jmarek@lab.nig.ac.jp Fri Mar 12 08:01:44 1999 *F To: rd120@gen.cam.ac.uk *F Subject: ADRC-417B *F Dear Dr. Drysdale, *F I reply to your inquiry about mbf1 gene, forwarded to me by Dr. Yasushi *F Hiromi. Sorry for not contacting you earlier. *F I would like to add to the information that Yash has already given you. *F mbf1 maps distal to tra, between RIGHT breakpoints of two deficiencies: *F Df(3L)st-e4 (mbf1 not included) and Df(3L)st7P (includes mbf1), i.e. *F between 73A5 and 73A7. *F Sincerely, *F Marek Jindra # *U FBrf0107806 *a Kennison *b J. *t 1999.2.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Feb 11 17:04:07 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Kennison stocks *F Hi folks-- *F We recently received a batch of stocks from Jim Kennison and there are a *F few things you should know. The information came from Kennison along with *F the stocks. I can fax the note if you want it. *F 1. Eip74EFv4 vtd4/TM3, st24 Sb1 *F This stock contains a new Eip74EF allele that was probably induced at the *F same time as vtd4. The mutation fails to complement Eip74EFneo24. *F The TM3, st24 Sb1 chromosome is a new balancer variant. Also marked *F with the standard TM3 markers ri1 pp sep1 l(3)89Aa1 bx34e e1. *F 2. Taf1101/TM6C, cu1 Sb1 ca1 *F Taf110 is allelic to l(3)72Dj. Taf1101 was isolated as l(3)72Dj1 and *F is listed under that name in FB now. *F 3. Df(3L)XS-533/TM6B, Sb1 Tb1 ca1 *F A Df isolated in the Rubin lab. Their cytology is 76B4;77B. *F TM6B, Sb1 Tb1 ca1 is a new balancer variant. Also marked with Hu and e. *F Thanks *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0107822 *a Krasnow *b M. *t 1999.3.3 *T personal communication to FlyBase *u *F From krasnow@cmgm.stanford.edu Wed Mar 03 18:20:42 1999 *F Date: Wed, 3 Mar 1999 10:20:09 -0800 *F To: Rachel Drysdale (Genetics) *F Rachel, Answer below... Mark *F >Dear Mark, *F > *F >We are currently curating the abstracts for the upcoming Bellevue *F >(Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F >writing in connection with your abstract: *F > *F >'stumps, a Drosophila gene required for FGF-directed migrations of *F >tracheal and mesodermal cells.' *F > *F >You mention a gene that is new to FlyBase, stumps. *F > *F >Do you have a map location for stumps? It is nice if we can keep as many *F >gene records as possible anchored to the map. *F We have a paper coming out on stumps in Genetics in May. From the *F manuscript... *F The stumps1 mutation was localized to cytological interval 88D3-4 to *F 88D14-15 on the right arm of chromosome III by complementation tests with a *F set of chromosomal deficiencies that remove different regions of the *F chromosome (Fig. 2B,C). No cytological abnormalities were detected in this *F region of the stumps1 chromosome (Fig. 2A), indicating that the mutation *F might be a small deletion or point mutation. Meiotic recombination mapping *F was used to localize stumps with respect to other genes in the region and *F to several RFLP markers that we identified. This analysis placed stumps *F 0.21 cM distal to crossveinless-c (cv-c) and 0.18 cM proximal to Pw+, *F lacZ3263 (Fig. 2D). *F > *F >Your last sentence 'Two recently described genes, (dof) and (hbr), *F >appear to correspond to the same locus' can be interpreted in more than *F >one way. We already know that hbr and dof are the same locus - are you *F >saying that stumps is the same as hbr as well? *F Yes, we have recently established this by complementation tests with hbr *F and dof; the dof allele derived from the same mutant chromosome as the *F stumps1 mutation. We propose retaining the name stumps because we used it *F in an earlier paper (Cell 87, 1091 (1996)) and also I believe in past *F abstract(s)/posters at the Annual Drosophila Conference. *F > *F >Thank you for your help, *F > *F >with best wishes, *F > *F >Rachel. *F > *F >---------------------------------------------------------------------- *F >Rachel Drysdale, Ph.D. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: rd120@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- # *U FBrf0107824 *a Krishnan *b K.S. *c A. *d Basole *t 1999.4.2 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Apr 01 16:01:12 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Apr 1999 16:01:12 +0100 *F To: ksk@tifr.res.in *F Subject: FlyBase query: ARDC40 - 872A *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 1 Apr 1999 16:03:55 +0100 *F Content-Length: 877 *F Dear Dr. Krishnan, *F I am curating the addendum book to the 40th Annual Drosophila Research *F Conference which was just held in Seattle. In your abstract: *F 'Characterization of novel, autosomal, temperature-sensitive paralytic *F mutants of Drosophila.' *F you describe two genes which are new to FlyBase, Kum and mkf. *F Do you have a map location for Kum and mkf? It is nice if we can keep *F as many gene records as possible anchored to the map. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From ksk@tifr.res.in Thu Apr 01 17:24:12 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Apr 1999 17:24:12 +0100 *F Date: Thu, 1 Apr 1999 21:59:40 +0530 (IST) *F From: K S Krishnan *F To: Gillian Millburn *F Subject: Re: FlyBase query: ARDC40 - 872A *F MIME-Version: 1.0 *F Dear Gillian, *F The polytene location of Kum is 60A1-7 , the lethality of homozygous Kum *F is uncovered by Df(2R)bw-S46 and Df(2R)or-BR11 but not by Df(2R)59AD or *F Df(2R)eg12 or Df(2R)Px1. *F We have done recombination mapping of mkf with rucuca chromosome and it *F is between stripe and ebony on the third chromosome. Recombonatipon *F mapping with P insertions in this region puts it at about 92-93 polytene *F region and since defeciencies Df(3R)D1-X12 or Df(3R) eR1 do not uncover *F it is most likely between 92D3 and 93B3. *F Since i am now on a visit to teh Us I have asked my student to write back *F to you if he has more updated information on mkf. *F Thanks very much *F krishnan *F On Thu, 1 Apr 1999, *F Gillian Millburn wrote: *F > Dear Dr. Krishnan, *F > *F > I am curating the addendum book to the 40th Annual Drosophila Research *F > Conference which was just held in Seattle. In your abstract: *F > *F > 'Characterization of novel, autosomal, temperature-sensitive paralytic *F > mutants of Drosophila.' *F > *F > you describe two genes which are new to FlyBase, Kum and mkf. *F > *F > Do you have a map location for Kum and mkf? It is nice if we can keep *F > as many gene records as possible anchored to the map. *F > *F > I look forward to hearing from you, *F > *F > Gillian *F > *F > -------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > -------------------------------------------------------------- *F > *F From amit@tifr.res.in Fri Apr 02 18:51:31 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 2 Apr 1999 18:51:31 +0100 *F Date: Fri, 2 Apr 1999 23:27:01 +0530 (IST) *F From: Amit Arun Basole *F To: gm119@gen.cam.ac.uk *F Subject: map positions of kum and mkf *F MIME-Version: 1.0 *F Dear Dr.Millburn, *F My name is Amit Basole and I work in Prof.Krishnan's lab at *F TIFR,Bombay. THe map positions of the two genes that we report are as *F given below- *F Kumbhakarna(Kum) (2-106%)- 60A1-7 (fails to complement *F Df(2R)bw-S46 and Df(2R)or-BR11, and complements Df(2R)eg12 ) *F mokaefe(mkf)-(3-70%) about 0.4% recombination units from the Atp *F alpha locus at 93B2, hence it probably lies in the 93A-B region *F Do let me know if you have any doubts about this. *F Sincerely, *F Amit Basole *F Dept. of Biological Sciences *F Tata Institute of Fundamental Research, *F Homi Bhabha Road, Colaba, *F Bombay-5, INDIA *F email- amit@tifr.res.in # *U FBrf0107835 *a Labourier *b E. *t 1999.3.19 *T personal communication to FlyBase *u *F Date: Fri, 19 Mar 1999 15:35:02 -0500 (EST) *F From: manucb@uclink4.berkeley.edu (Emmanuel Labourier) *F Subject: FlyBase Help Mail *F To: flybase-help@morgan.harvard.edu *F manucb@uclink4.berkeley.edu (Emmanuel Labourier) sent the following *F comments to flybase-help: *F \------------------------------------------------------------ *F Gene symbol Rox21 *F Full name Rox21 *F FlyBase ID number FBgn0011305 *F Report content Full Graphic map *F Synonym(s) ROX21 *F Date 03 Mar 99 *F Cytological location 31C1-31D6 (determined by in situ *F hybridization) *F ROX21 protein has been renamed RSF1 (repressor splicing factor 1) *F Ref: Emmanuel Labourier, Henri-Marc Bourbon, Imed-eddine Gallouzi, *F Maggy Fostier, Eric Allemand, and Jamal Tazi *F Antagonism between RSF1 and SR proteins for both splice-site *F recognition in vitro and Drosophila development *F Genes and Development (1999) 13(6) *F \------------------------------------------------------------ # *U FBrf0107839 *a Lasko *b P. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:20:55 1999 *F To: Paul_Lasko@maclan.mcgill.ca *F Subject: ADRC-490C *F Dear Paul, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'The Drosophila Homologue of yIF2 Interacts with VASA.' *F You mention a gene that is new to FlyBase, IF2. *F Do you have a map location for IF2? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F (ps Hi Paul, I would ordinarily have sent this to first author O. *F Johnstone but said O. Johnstone is not in the FlyBase people *F directory, so you are the lucky recipient. Will you be in Bellevue? I *F will look out for you.) *F From plasko1@maclan.mcgill.ca Wed Mar 03 14:01:38 1999 *F Subject: Re: ADRC-490C *F To: 'Rachel Drysdale' *F Hello Rachel, good to have word from you. We are calling this gene cIF2 *F for cytoplasmic IF2 to distinguish it from mitochondrial IF2. It is *F located at 63D2-3. I look forward to seeing you in Bellevue. All the *F best, Paul. # *U FBrf0107840 *a Lasko *b P. *t 1999.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:41:18 1999 *F To: Paul_Lasko@maclan.mcgill.ca *F Subject: ADRC-737A *F Dear Paul, *F We are (still) currently curating the abstracts for the upcoming *F Bellevue (Seattle) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of A11, a VAS-interacting protein' *F You mention a gene that is new to FlyBase, A11. *F Do you have a map location for A11? It is nice if we can keep as many *F gene records as possible anchored to the map. Have you settled on a *F nicer symbol than A11 for this gene (or maybe you think that A11 is a *F beautiful symbol in which case A11 it shall be .....) *F Thank you for your help, *F with best wishes, *F Rachel. *F From plasko1@maclan.mcgill.ca Wed Mar 03 14:07:48 1999 *F Subject: Re: ADRC-737A *F Date: Wed, 3 Mar 1999 09:14:26 -0400 *F Dear Rachel, We will call this gene gustavus (gus) since that was King *F Vasa's first name. Unfortunately the chromosomal location remains *F uncertain...we are trying to sort out a discrepancy between our cytology *F and the reported map location of an unfinished BDGP clone which we hope *F is chimeric. *F All the best, Paul. # *U FBrf0107850 *a Lewis *b E. *t 1999.2.9 *T personal communication to FlyBase *u *F From: Kevin Cook *F Subject: In(3LR)64;84 *F Hi Ed-- *F I recently received a stock from Jim Kennison that has a chromosome in it *F that you constructed. The chromosome was described in a paper from Ian *F Duncan (Hopmann et al., Genetics 1995 139(2):815--833, Transvection in the *F iab-5,6,7 region of the bithorax complex of Drosophila: Homology *F Independent interactions in trans) as *F Dp(3;3)S462 + In(3LR)64;84 + In(3R)C *F In a DIS note (D. I. S. 1980 55:207--208), you described Dp(3;3)S462 as an *F insertion of 89D1-2;90D1 into 64C-E, but didn't mention an inversion of *F 64;84 on the chromosome. Was the inversion induced at the same time as the *F Dp(3;3)? *F We try to resolve questions about stocks before we start distributing them. *F I hope you can help with this little problem. I'll update FlyBase with *F whatever information you can provide. *F Best regards, *F Kevin *F At 12:20 AM 2/9/99 -0800, you wrote: *F >Hi Kevin, Still trying to trace back the source of balancer. . I think it was *F >In(3lr) induced with x-rays on a DpS462 + 3R C chromosome and possibly with Sb *F >Tb markers. I no longer have the balancer and am glad to know it exists. *F >Does *F >Jim's stock have Sb Tb? *F >Ed *F > *F > *F > *F >Edward B. Lewis *F >Professor of Biology, Emeritus (ACTIVE) *F >Biology Division, 156-29, Caltech *F >Pasadena, CA 91125 *F >Phone: 626-395-4941 *F >FAX: 626-564-9685 # *U FBrf0107861 *a Lorenzen *b J. *t 1999.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:56:21 1999 *F To: Lorenzen@helix.mgh.harvard.edu *F Subject: ADRC-134 *F Dear James, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Corkscrew interacts with the putative MAPK nuclear import protein DmRanBP7.' *F You mention a gene that is new to FlyBase, RanBP7. *F Do you have a map location for RanBP7? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From lorenzen@helix.mgh.harvard.edu Thu Mar 04 22:42:42 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-134 *F Dear Rachel, *F With regards to your email asking about RanBP7, we have decided to *F rename the gene dim-7 which stands for D-Importin 7/RanBP7. *F dim-7 lies in the chromosomal region 66B8/9-B10. *F Best Wishes, *F James Lorenzen *F From rd120@gen.cam.ac.uk Wed Mar 10 11:53:04 1999 *F To: lorenzen@helix.mgh.harvard.edu *F Subject: Re: ADRC-134 *F Dear James, *F Thank you very much for your prompt and helpful reply. Your message *F will be curated as a personal communication from you to FlyBase. There *F is a problem however with the symbol. Renaming is fine but we cannot *F prefix symbols in FlyBase with d or D for Drosophila. Also, genes that *F are named for proteins generally have the first letter capitalized. *F This leaves us with Im-7:Importin-7 for the symbol:name. How does that *F sound? *F Best regards, *F Rachel. *F From lorenzen@helix.mgh.harvard.edu Thu Mar 11 18:36:01 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-134 *F Dear Rachel, *F We chose the name DIM-7 to describe the strikingly DIMinished *F activated MAP kinase immunostaining that we observe in embryos mutant for *F Importin 7/RanBP7. Therefore, we would very much like to keep this name; *F however, we also want to be consistent with fly base. Please advise. *F Thanks very much. *F James Lorenzen *F From rd120@gen.cam.ac.uk Fri Mar 12 10:02:07 1999 *F To: lorenzen@helix.mgh.harvard.edu *F Subject: Re: ADRC-134 *F Dear James, *F >We chose the name DIM-7 to describe the strikingly DIMinished *F >activated MAP kinase immunostaining that we observe in embryos mutant for *F >Importin 7/RanBP7. Therefore, we would very much like to keep this name *F That is a valiant effort of reasoning. I will record the gene symbol *F as dim-7 (as in your mail of last Thursday (4th)) and make a note in *F the Etymology as to why it has this symbol. *F Regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0107864 *a Lukacsovich *b T. *t 1999.3.15 *T personal communication to FlyBase *u *F >From lukacs@fly.erato.jst.go.jp Mon Mar 15 11:17:31 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 15 Mar 1999 11:17:31 +0000 *F Date: Mon, 15 Mar 1999 20:21:27 +0900 *F To: Michael Ashburner (Genetics) *F From: lukacs@fly.erato.jst.go.jp (Tamas Lukacsovich) *F X-Sender: lukacs@202.241.18.161 *F Subject: Re: Help FlyBase please *F MIME-Version: 1.0 *F X-Mailer: Eudora-JE(1.3.8-JE13) *F It's another Man-II. I mean a new putative golgi 1,3-1,6-alpha-mannosidase *F at 89A1-A5. *F May it be named as alpha-M-II ? *F (I am just considering to write a paper about the possible role of two *F separated genes with apparently identical function.) *F Regards, *F Tamas Lukacsovich *F At 8:27 AM 99.3.15 +0000, Michael Ashburner (Genetics) wrote: *F >I see you have sequenced a fly alpha-mannosidase (nucleic *F >acid accession number AB018079). *F > *F >Can you give this a gene symbol please ? *F > *F >FlyBase already knows about these mannosidases: *F > *F >&agr;-Man-4 *F >FBgn0022716 *F >alpha-mannosidase == EC 3.2.1.24 *F > *F > *F >&agr;-Man-I *F >FBgn0010338 *F >9B7--8 *F >mannosyl-oligosaccharide 1,2-alpha-mannosidase == EC 3.2.1.113 *F > *F >&agr;-Man-II *F >FBgn0011740 *F >85D14--17 *F >mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase == EC 3.2.1.114 *F > *F > *F > *F >Thanks for your help. *F > *F >Michael Ashburner # *U FBrf0107868 *a Maeland *b A. *c N. *d Brown *t 1999.3.15 *T personal communication to FlyBase *u *F From nb117@mole.bio.cam.ac.uk Mon Mar 15 20:16:52 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Sep4 *F Hi Rachel, *F I can confirm the location of Sep4 at 15A1-2, which is work done by Anne *F Maeland and myself. *F Best wishes, Nick *F Nick Brown *F Wellcome/CRC Institute *F Tennis Court Road *F Cambridge CB2 1QR *F England # *U FBrf0107878 *a Mattox *b W. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:46:28 1999 *F To: wmattox@molgen.mdacc.tmc.edu *F Subject: ADRC-19W *F Dear William, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'A role for protein phosphorylation in Drosophila sex determination.' *F You mention a gene that is new to FlyBase, SRPK (dSRPK, we must drop *F the 'd' - we do not prefix with d for Drosophila in FlyBase). *F Do you have a map location for SRPK? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From wmattox@molgen.mdacc.tmc.edu Wed Mar 03 16:34:28 1999 *F Subject: Re: ADRC-19W *F To: 'Genetics' *F Reply to: RE>ADRC-19W *F Dear Rachel, *F I'm happy to share the info. We have mapped Drosophila SRPK to the P1 clone DS03596 which is reported to be at 79D4. *F Bill # *U FBrf0107884 *a Megraw *b T. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:43:48 1999 *F To: tmegraw@bio.indiana.edu *F Subject: ADRC-062 *F Dear Tim, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'The centrosomin locus encodes four isoforms, one of which is testis *F specific and arrests cell division in mitosis when expressed *F ectopically.' *F You mention a gene that is new to FlyBase, dem. *F You say that dem is very tightly linked to cnn. I imagine that this *F map statement is based on molecular mapping, for example because the *F dem transcription unit maps next to the cnn transcription unit. Would *F you be able to confirm this for me, or otherwise give me a basis for *F the map statement? It is nice if we can keep as many gene records as *F possible anchored to the map, and I need to be able to formalise the *F map statement if we are to be able to usefully record the location of *F dem with respect to cnn. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tmegraw@bio.indiana.edu Wed Mar 03 21:32:58 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-062 *F Dear Rachel, *F Thanks for contacting me regarding 'dem', which I mentioned in my abstract. *F We (Thom and I) are still undecided on what to name this gene and dem *F (short for dementin) may be inappropriate considering what we now know *F about the gene's function. Thom will call you tomorrow (Thurs) to discuss. *F This gene maps VERY close to cnn physically. The two genes are divergently *F transcribed and the transcription start sites are separated by a mere 134 *F bp. *F Sincerely, *F Tim *F From rd120@gen.cam.ac.uk Mon Mar 08 16:29:27 1999 *F To: tmegraw@bio.indiana.edu *F Subject: Re: ADRC-062 *F Hi Tim, *F I gather after my phone conversation with Thom that this is what is to *F be 'centrosomin's beautiful sister'. Could you confirm this for me *F (sorry to be a nuisance - we have to have everything in writing *F otherwise chaos ensues....)(even more than otherwise ......) *F Rachel. *F From tmegraw@bio.indiana.edu Mon Mar 08 20:37:29 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-062 *F Hi Rachel, *F Sorry I forgot to get back to you til now. I am very much in favor of *F calling this new gene 'centrosomin's beautiful sister' (cbs), as *F opposed to the name 'dementin' which was mentioned in the abstract I *F submitted to the Drosophila Research Conference. I thank you much for *F you help in settling this issue for us. The new name is very *F appropriate. *F Tim # *U FBrf0107897 *a Murphy *b T. *t 1999.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:33:52 1999 *F To: tdmurphy@ucsd.edu *F Subject: ADRC-682C *F Dear Terence, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Analysis of the roles of a family of Drosophila skp1 homologs in cell *F cycle progression' *F You mention four genes that are new to FlyBase, skpA, B, C and D. *F Do you have a map location for any of these genes? It is nice if we *F can keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tdmurphy@ucsd.edu Wed Mar 10 19:23:53 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-682C *F Dear Rachel, *F Thanks for your interest in the Drosophila skp genes. A lot of the *F information for dskpA ties into the Berkeley database. EP(X)1423 is *F inserted into the 5' UTR of dskpA, originally indicated by end sequencing *F done by the BDGP. The sequence from EP(X)1423 also ties into the sequence *F of cosmid 115C2, in which the EDGP identified an ORF homologous to SKP1. *F There are also a bunch of ESTs, but I'm not sure you keep those on the *F Flybase map. I'll add that dskpA is located at 1B10-14 on the polytene *F map, so you can also anchor that cosmid sequence. Imprecise excisions of *F EP(X)1423 are lethal, and fail to complement: *F Df(1)svr *F T(1;Y)B106, y (the Df(1) half) *F Df(1)su(s)83 *F but are complemented by: *F Df(1)y74k24.1 *F The complementation data is consistent with the polytene localization. *F As far as the other homologs, I'll send along that information when I *F publish them, since I know other people are interested in the SKP1 genes *F and may not know about the other family members. I have no objections to *F you adding the above data to Flybase at this point, since almost all of it *F can be derived from the BDGP data anyway (except for the polytene *F localization). Let me know if you want any other pieces of data clarified. *F \-Terence *F From rd120 Thu Mar 11 12:36:50 1999 *F To: murphy@biomail.ucsd.edu *F Subject: Re: ADRC-682C *F Dear Terence, *F Thank you very much for the information about skpA. It is very useful *F indeed and allows me to rename the EG:115C2.4 gene (FBgn0025637) that *F is currently in FlyBase as skpA. I see no reason to doubt that they *F are the same thing. Not only that, but I can link the insertion we *F currently have as P{EP}EP1423 (FBti0007441) to an allele of skpA, which *F we should call skpAEP1423, to reflect the insertion into the 5' UTR *F (even if the insertion is phenotypically silent). *F Brilliant. *F We do indeed capture links between ESTs and genes so if it is easy for *F you to provide me with the relevant EST identifiers that would be the *F icing on the cake. *F Your mail will be curated as a personal communication to FlyBase. *F Many thanks, *F Rachel. # *U FBrf0107898 *a Murthy *b J. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:59:41 1999 *F To: murthy@morgan.harvard.edu *F Subject: ADRC-176A *F Dear Jay, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Identification and genetic characterization of Su(dpp)39CDE, a *F dominant maternal effect suppressor of decapentaplegic.' *F You mention a gene that is new to FlyBase, Su(dpp)39CDE. Do you have *F an allele symbol for the EMS allele e.g. Su(dpp)39CDE1? *F You mention a map location for Su(dpp)39CDE, but do not say how that *F location was determined. Would it be possible for you to give us a *F little more so that the mapping info can be stored more robustly in *F FlyBase? It looks like it was probably delimited by deficiency breaks, *F or similar. Could you tell us which aberrations were used? *F Thank you for your help, *F with best wishes, *F Rachel. *F From murthy@fas.harvard.edu Wed Mar 03 20:22:31 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-176A *F Hello Rachel, *F The genetics here are atypical. The mutant, Su(dpp)39CDE, is actually *F a dominant maternal effect suppressor of the dpp haploinsufficiency *F phenotype, but it turns out that homozygotes are perfectly viable and *F fertile on their own. *F The mapping data arises from the following peculiar observation: *F homozygous Su mothers will enhance 3X and 4X dpp copy number. That is, *F flies bearing 3 or 4 wildtype copies of dpp will die with greater *F frequency compared to their 2X sibs when their mother is Su homozygous. *F Astonishingly, and most relevant to the mapping discussion, Su/Df also *F gives the same enhancement result, even though Df/+ neither suppresses *F haploinsufficiency nor enhances >2X dpp. *F Therefore, the Su is probably a gain of function mutation, and Su/Df *F mothers behave as Su/Su mothers, at least with regard to enhancement of *F >2X dpp. My speculation is that the presence of a wildtype copy of this *F gene ameliorates the effects of the mutant on >2X dpp, hence the *F observation that Su/+ mothers do not enhance. *F The Df's over which the enhancement effect is observed are: *F TW161 *F TW84 *F TW65 *F The enhancement effect is NOT observed over: *F TW1 *F TW12 *F ...hence the estimated cytological position. *F As for an allele symbol, Su(dpp)39CDE1 makes sense to me. *F Best Regards, *F Jay. # *U FBrf0107912 *a Nitasaka *b E. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk To: enitascb@mbox.nc.kyushu-u.ac.jp *F Subject: ADRC-654B *F Dear Eiji, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'mgr, a novel gene involved in DNA repair and recombination.' *F You mention a gene that is new to FlyBase, mgr (mogura). Unfortunately *F the 'mgr' symbol is already taken (merry-go-round), so we will have to *F find a new symbol for mogura. mog is a possibility - how does that *F sound to you? *F Do you have a map location for mgr/mog? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From enitascb@mbox.nc.kyushu-u.ac.jp Wed Mar 03 11:43:35 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-654B *F Dear Rachel: *F Thank you very much for your information. We will change the mgr to mog. It sounds good. mog gene is located around 74C on the 3L. *F I appreciate again your info. *F Sincerely. *F \----------------------------------------- *F Eiji Nitasaka *F Department of Biology *F Faculty of Science, Kyushu University *F & PRESTO, JST *F Fukuoka 812-8581, Japan *F Phone: +81-92-642-2616 *F Fax: +81-92-642-2645 *F email: enitascb@mbox.nc.kyushu-u.ac.jp *F web: http://mg.biology.kyushu-u.ac.jp/ *F \----------------------------------------- # *U FBrf0107913 *a O'Brien *b N.W. *t 1999.3.25 *T personal communication to FlyBase *u *F Archived. # *U FBrf0107914 *a O'Connor *b M. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:10:58 1999 *F To: moconnor@gene.med.umn.edu *F Subject: ADRC-363 *F Dear Michael, *F We are (still) curating the abstracts for the upcoming Bellevue (Seattle) *F Annual Drosophila Research Conference, for FlyBase. I am writing in connection with *F your abstract: *F 'Isolation and characterization of dSARA, a D. melanogaster homologue *F of hSARA which enhances Smad2/TGF- receptor interactions.' *F You mention a gene that is new to FlyBase, Sara. *F Please could you let me know which EST is identified with Sara? *F If we know these things we can make the proper links in FlyBase. *F Thank you for your help, *F with best wishes, *F Rachel. *F From moconnor@lenti.med.umn.edu Wed Mar 03 15:32:25 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-363 *F Hi Rachel, *F The ESTs are LD13137 and ck01010 *F M. O'Connor # *U FBrf0107915 *a O'Connor *b M. *t 1999.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:10:46 1999 *F To: moconnor@gene.med.umn.edu *F Subject: ADRC-362A *F Dear Michael, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'The role of TAK1 in Drosophila development.' *F You mention a gene that is new to FlyBase, Tak1. *F Do you have a map location for Tak1? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From moconnor@lenti.med.umn.edu Wed Mar 03 15:34:35 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-362A *F Dear Rachel, *F Tak1 maps to 19E1-4 *F M. O'Connor # *U FBrf0107923 *a Page *b S. *t 1999.3.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:03:35 1999 *F To: slpage@ucdavis.edu *F Subject: ADRC-280C *F Dear Scott, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'mei-P26, a putative transcriptional regulator of meiosis and oogenesis.' *F You mention a gene that is new to FlyBase, mei-P26. *F Do you have a map location for mei-P26? It is nice if we can keep as *F many gene records as possible anchored to the map. Do you have a *F symbol for the mutant allele of mei-P26, such as mei-P261? Can you *F tell me what P element is inserted in the mutant (e.g. P{lacW}, or is *F it just a regular P element)? *F Thank you for your help, these details all help to make the information *F in FlyBase neat, tidy and complete. *F with best wishes, *F Rachel. *F From slpage@ucdavis.edu Thu Mar 11 18:13:02 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-280C *F Dr. Drysdale, *F >'mei-P26, a putative transcriptional regulator of meiosis and oogenesis.' *F > *F >You mention a gene that is new to FlyBase, mei-P26. *F > *F >Do you have a map location for mei-P26? It is nice if we can keep as *F >many gene records as possible anchored to the map. *F 8C10-8D3 *F left end based on left end of Df(1)18.1.15 *F right end based on left end of Df(1)10-70d *F > Do you have a *F >symbol for the mutant allele of mei-P26, such as mei-P261? *F mei-P261 *F > Can you *F >tell me what P element is inserted in the mutant (e.g. P{lacW}, or is *F >it just a regular P element)? *F mei-P261 is an insertion of P{lacW} # *U FBrf0107938 *a Philp *b A. *t 1999.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:34:21 1999 *F To: ajvphilp@scotlandmail.com *F Subject: ADRC-695A *F Dear Alastair, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'WHY WE FRET: THINK AGAIN ABOUT DROSOPHILA CENP-E PROTEINS IN MITOSIS' *F You mention two genes new to FlyBase, fret and thag. *F Do you have map locations for fret and thag? It is nice if we can keep *F as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From ajvphilp@scotlandmail.com Fri Mar 05 23:13:41 1999 *F To: rd120@gen.cam.ac.uk *F Subject: ADRC-695A (fret and pana?) *F Dear Rachel, *F Sorry to have taken so long to respond to your e-mail (reproduced *F below). The names used in my abstract look likely to be changed and I *F have been discussing with my advisor and collaborators what they will *F end up being. *F It is likely that they will be free thinker (fret; fails to align its *F chromosomes with the established position) and pollyanna (pana; many *F more anaphases in mutant brains) but we reserve the right to change *F this, for example to DmCENP-E1 and DmCENP-E2 on publication. Since I *F would prefer not to generate any additional confusion to the community *F (or conflict with my coauthors) it would be best if any record in *F flybase is marked as likely to change. I don't know what procedures you *F have in place in order to deal with these sorts of issues but would *F welcome your advice. *F Incidentally to answer your initial question both genes (both Drosophila *F homologues of mammalian CENP-E) are in polytene 32E. *F Alastair Philp # *U FBrf0107954 *a Rinehart *b J. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:31:56 1999 *F To: rinehart@eou.edu *F Subject: ADRC-612B *F Dear John, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Grooming defects and complex expression of an enhancer trap mutation *F in D. melanogaster.' *F You mention a gene that is new to FlyBase, D5. *F Do you have a map location for D5? It is nice if we can keep as many *F gene records as possible anchored to the map. Do you have a gene *F symbol/name and allele symbol yet for this gene? *F Thank you for your help, *F with best wishes, *F Rachel. *F From rinehart@eou.edu Wed Mar 03 15:34:44 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-612B *F Hi Rachel; *F The mutation maps to autosome III at position 53.8, as determined by *F recombination mapping to ru, th, and cu. We did have a name picked out for *F it but are not sure the name is really appropriate; we thought of agee as *F the gene symbol and the name antenno-glial expressed, but it has become *F clear that the expression in the antennomaxillary complex is not in glial *F cells, although there appears to be glial expression elsewhere. This name *F will probably stick but I'll let you know for sure when we make a final *F decision. *F John Rinehart # *U FBrf0107956 *a Roote *b J. *t 1999.3.10 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Wed Mar 10 16:27:00 1999 *F To: rd120@mole.bio.cam.ac.uk *F Rachel, *F Another factoid: *F In(2LR)b71k1A (34C7;43C2) is lethal with l(2)43BcEw2 - one might assume *F because b71k1A breaks in 43Bc. *F John # *U FBrf0107957 *a Roote *b J. *t 1999.3.18 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Thu Mar 18 16:52:40 1999 *F To: rd120@mole.bio.cam.ac.uk *F Dear Rachel, *F New alleles: *F k06520 carries a 2nd site wb allele (in situ maps to 36D1-36D2) *F k00418 carries a 2nd site esg allele (in situ maps to 33E9-33E10) *F Not sure how you handle this one: *F k07018 in situ maps to 50C, sequence maps to esg, lethal with esg. *F Also: *F k11112 carries an stc allele, but it's not revertable by P element excision *F \- in situ maps to 35D1-35D4, which is closer to esg (stc is 35C1), so *F presumably a 2nd site. *F I originally called the 2nd site alleles IK# when on Kiss inserts and AS# *F when on PZs. I think we got up to rkIK6 and rkAS3. *F John # *U FBrf0107972 *a Schaefer *b U. *t 1999.4.15 *T personal communication to FlyBase *u *F >From uschaef@gwdg.de Thu Apr 15 09:01:54 1999 *F To: Michael Ashburner (Genetics) *F Subject: Re: Help FlyBase please *F Hi Michael, *F Yes, I can confirm this although the actual test wasn't done by myself. *F Adam N. Harris in Paul Macdonald's lab at Stanford did it and informed me *F about the non-complementation of aubergine and sting. I got their *F permission to make this information public. *F . *F . *F Best regards, *F Ulrich *F Dr. Ulrich Schaefer # *U FBrf0107979 *a Scully *b A. *t 1999.3.7 *T personal communication to FlyBase *u *F From ascully@scripps.edu Sun Mar 07 18:43:48 1999 *F To: kcook@bio.indiana.edu *F Subject: l(4)102CDh lines *F Cc: flybase-updates@morgan.harvard.edu *F For the l(4)102CDh analysis, the following fly lines were used: l(4)102CDh *F \#2704 from BG (now listed as l(4)102CDh-1 \#4041 at Midamerica), l(4)102CDh2 *F \#87200, and l(4)102CDh* \#73680 both from the Umea stock center. *F I tested these l(4)102CDh lines for allelic complementation and found that *F all three lines survive over one another. Therefore, it is highly unlikely *F that the three lines represent the same complementation group. Line \#4041 *F does not survive over \#2704 confirming that these are the same line *F according to the stock center. *F My further complementation analysis used lines \#2704, \#87200 & \#73680. *F Complementation analysis of ey recessive alleles, eyR and ey2, with the *F l(4)102CDh lines demonstrate that the l(4)102CDh lines should not be listed *F as ey alleles without specific evidence showing that they are ey alleles. *F None of the l(4)102CDh lines show lethality or a reduced eye phenotype over *F these recessive ey alleles. In addition, all three lethal lines survive *F over eyD, a suspected dominant ey allele which is homozygous lethal, and do *F not exhibit any exacerbation of the eye phenotype normally seen with eyD/+. *F All three l(4)102CDh lines survive over Df(4)G. # *U FBrf0108001 *a Son *b W. *t 1999.3.8 *T personal communication to FlyBase *u *F >From rd120 Wed Mar 3 11:12:04 1999 *F Subject: ADRC-343C *F Dear Wonseok Son, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'collier, a Transcription Factor of the COE Family, Is Required for the *F Larval Olfaction in Drosophila' *F You mention an enhancer trap allele that is new to FlyBase, col85. *F Could you tell us which enhancer trap element is inserted in this *F allele? If it is one of the ones that originates in the BDGP or Szeged *F collections then what is the indertion identifier number? If we know *F these things we can make the proper links in FlyBase. *F I realise that you are not necessarily the best choice of author for me *F to contact, but you are the first author for this abstract whose address *F I found in the FlyBase people file. If you want to pass it on to someone *F more appropriate, then please go ahead. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jjanga@plaza1.snu.ac.kr Mon Mar 08 09:26:35 1999 *F To: ''rd120@gen.cam.ac.uk'' *F Subject: Dear, Dr. Drysdale. *F Dear, Dr. Drysdale. *F The enhancer trap element in col85 is PlArB. *F I think that it is'nt necessary to explain more about the famous P-element. *F The mutant was generated in the previous screening of genes which express in the olfactory system in our lab. *F Don't hesitate if any more question. *F Sincerely. *F Wonseok Son # *U FBrf0108011 *a Stowers *b S. *t 1999.4.1 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Apr 01 16:03:48 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Apr 1999 16:03:48 +0100 *F To: sstowers@cmgm.stanford.edu *F Subject: FlyBase query: ARDC40 - 873B *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 1 Apr 1999 16:06:36 +0100 *F Content-Length: 894 *F Dear Dr. Stowers, *F I am curating the addendum book to the 40th Annual Drosophila Research *F Conference which was just held in Seattle. In your abstract: *F 'Genetic screen using a novel genetic technique for generating eye *F clones identifies milton, an ERG on/off transient mutant.' *F you describe a gene that is new to FlyBase, 'milton'. *F Do you have a map location for milton? It is nice if we can keep as *F many gene records as possible anchored to the map. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From sstowers@cmgm.stanford.edu Thu Apr 01 19:48:48 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Apr 1999 19:48:48 +0100 *F Mime-Version: 1.0 *F Date: Thu, 1 Apr 1999 10:51:46 -0800 *F To: Gillian Millburn (Genetics) *F From: Steve Stowers *F Subject: Re: FlyBase query: ARDC40 - 873B *F Hello, *F Milton maps to the 27CD region of the genome. Moreover, the P-element *F alleles l(2)K06704 and l(2)K14514 are located at nearly the same position *F in the first intron of the milton gene. The only reservation I have about *F putting this info in Flybase is that after further phenotypic *F characterization and before publication we may decide to change the name of *F the gene to one that better describes what we think the gene is doing. I *F guess if that happens Flybase would just get updated at that point so that *F the gene name gets changed, so go ahead. *F Steve *F Steve Stowers *F Postdoctoral Fellow *F Department of Molecular and Cellular Physiology *F Beckman Center, Room 117 *F Stanford University School of Medicine *F Stanford, CA 94305 *F (650) 725-7773 # *U FBrf0108013 *a Stronach *b B. *t 1999.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:06:36 1999 *F To: bstronach@rascal.med.harvard.edu *F Subject: ADRC-324B *F Dear Beth, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'slipper, an X-linked locus required for dorsal closure.' *F You mention a gene that is new to FlyBase, slpr. *F Do you have a map location for slpr? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From bstronach@rascal.med.harvard.edu Thu Mar 04 16:13:48 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-324B *F Hello Rachel: *F Based on deficiency mapping, i can place slpr either, 7D13-14 or 7D21-7E1. *F This should become more firm in a few weeks since i am pursuing analysis of *F recombinant chromosomes between sn and slpr. (I am favoring the more proximal *F position of 7D21-7E1) *F Fails to complement Df(1)GE202 and Df(1)hl-a, but complements Df(1)HA11. *F Beth # *U FBrf0108041 *a Uemura *b T. *t 1999.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:01:53 1999 *F To: tuemura@take.biophys.kyoto-u.ac.jp *F Subject: ADRC-240B *F Dear Tadashi, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'The making of a leaf and a wing: roles of dAGO1, a homolog of *F Arabidopsis Argonaute1.' *F You mention a gene that is new to FlyBase, AGO1. *F Do you have a map location for AGO1? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tuemura@take.biophys.kyoto-u.ac.jp Fri Mar 12 00:58:33 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-240B *F Dear Rachel. *F >'The making of a leaf and a wing: roles of dAGO1, a homolog of *F >Arabidopsis Argonaute1.' *F > *F >You mention a gene that is new to FlyBase, AGO1. *F > *F >Do you have a map location for AGO1? *F Please note that our gene name is dAGO1, not AGO1. It is in 50C. *F I am sorry that I did not reply to you soon. I have been hectic and *F confused two emails from you. *F Sincerely, *F Tadashi # *U FBrf0108042 *a Uemura *b T. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:53:22 1999 *F To: tuemura@take.biophys.kyoto-u.ac.jp *F Subject: ADRC-099 and 213B *F Dear Tadashi, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstracts: *F 'Opposite activities of Flamingo and Frizzled, serpentine receptors of *F two distinct families, in specifying planar cell polarity.' *F and *F 'Serpentine receptors of two distinct families, Flamingo and Frizzled, *F exert opposite activities in specifying planar cell polarity.' *F You mention a gene that is new to FlyBase, fmi. *F Do you have a map location for fmi? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tuemura@take.biophys.kyoto-u.ac.jp Wed Mar 03 11:03:10 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-099 and 213B *F Dear Rachel, *F >You mention a gene that is new to FlyBase, fmi. *F > *F >Do you have a map location for fmi? It is nice if we can keep as many *F >gene records as possible anchored to the map. *F flamingo (fmi) is located in 47B. Thank you very much for your efforts. *F Sincerely, *F With best regards, *F Tadashi Uemura # *U FBrf0108057 *a Voigt *b W. *t 1999.3.25 *T personal communication to FlyBase *u *F Archived. # *U FBrf0108069 *a White-Cooper *b H. *t 1999.3.3 *T personal communication to FlyBase *u *F From helen.white-cooper@zoology.oxford.ac.uk Wed Mar 03 16:43:03 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-120 *F >Dear Helen, *F > *F >We are currently curating the abstracts for the upcoming Bellevue *F >(Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F >writing in connection with your abstract: *F > *F >'The meiotic arrest gene always early (aly) encodes a nuclear protein *F >that may regulate ras signalling in the Drosophila testis.' *F > *F >You mention two genes that are new to FlyBase, zaa and zab. *F > *F >Do you have map locations for zaa and zab? It is nice if we can keep *F >as many gene records as possible anchored to the map. *F > *F >Thank you for your help, *F > *F >with best wishes, *F > *F >Rachel. *F > *F zaa and zab are zuker-aly-like a and b. I don't necessarily want to use *F these as the final name for the genes. *F zaa is at 65.5 +/- 3.3 on the second chromosome based on recombination *F between cn and c. it is also uncovered by Df number 442 from the deficiency *F kit ( I don't have the name to hand). This places it in 49C-50C. *F zab is also on the second. I didn't calculate the position accurately by *F recombination, but it is around 96, based on recomb between c and px. It *F is uncovered by Df(2R)R1-8 and Df(2R)X58-7, but not X58-9. This places it *F in 58A1-B2. *F Hope this helps. *F Helen *F \-------------------------------------------------------------------------------- *F \-------------------------------------------------------------------------------- *F Helen White-Cooper *F Dept of Zoology *F University of Oxford *F South Parks Road *F Oxford *F OX1 3PS # *U FBrf0108071 *a Whittaker *b A.J. *t 1999.3.27 *T personal communication to FlyBase *u *F Archived. # *U FBrf0108076 *a Wilson *b C. *t 1999.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 10:41:28 1999 *F To: C.Wilson@ukc.ac.uk *F Subject: ADRC-035 *F Dear Clive, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'The Drosophila homologue of the human tumour suppressor PTEN regulates *F cell size and proliferation via the actin cytoskeleton.'. *F You mention a gene that is new to FlyBase, Pten. *F Do you have a map location for Pten? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F From C.Wilson@ukc.ac.uk Thu Mar 04 09:03:29 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-035 *F Dear Rachel *F DPTEN maps to 31B/C, possibly 31C1. It has very recently been sequenced by *F BDGP (AC005454). This P1 clone contains primarily proximal 2L sequence - *F Dror lies just a few kilobases distal to DPTEN. We are waiting to hear *F about the fate of a manuscript submitted on this gene, before submitting *F the cDNA sequence. *F If you require any further information, please let me know. *F Best wishes *F Clive # *U FBrf0108079 *a Wolf *b T. *t 1999.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:32:16 1999 *F To: zzwolf@acc.wuacc.edu *F Subject: ADRC-613C *F Dear Thomas, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'A mutational change affecting fluorescent facial patterns.' *F You say 'A recessive mutation which has pleiotropic *F effects has been induced in D. melanogaster using EMS.'. *F Do you have a gene symbol/name and allele symbol yet for your gene and *F its mutant form? We cannot make a record for it if it doesn't have *F some kind of name. *F Thank you for your help, *F with best wishes, *F Rachel. *F From zzwolf@washburn.edu Tue Mar 23 14:37:09 1999 *F To: Rachel Drysdale *F Subject: Re: ADRC-613C *F Rachel: *F The mutation we have found is an autosomal recessive which appears to *F produce a pronounced trident on the dorsal thorax. It appears to have *F variable expressivity (1-5, least to most) and at level 4 & 5 produces *F abnormal wing morphology and significantly reduces the intensity of the *F fluorescent facial patterns that we have been studying. The student who *F found the mutation has named it retiarius (ret). Preliminary studies *F suggest it may be on chromosome \# 4. *F Tom Wolf *F From rd120@gen.cam.ac.uk Wed Mar 31 12:09:58 1999 *F To: zzwolf@washburn.edu *F Subject: Re: ADRC-613C *F Dear Tom, *F Many thanks for getting back to me. *F Your message is full of useful data and I will curate it as a personal *F communication from you to FlyBase. One small problem - the symbol ret *F is preoccupied (reticulated) , as is reti (reticent) so I will use rets *F for retiarius unless you have another suggestion. *F Sorry to be so long in getting back to you - we were in Seattle for a *F FlyBase project meeting for days before the Research Conference started *F so I didn't get your message until I got back, yesterday. *F With best wishes, *F Rachel. # *U FBrf0108087 *a Yagi *b Y. *t 1999.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:28:28 1999 *F To: mack@nibb.ac.jp *F Subject: ADRC-560A *F Dear Makoto, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Analysis of Alzheimer related genes in Drosophila.' *F You mention two genes that are new to FlyBase, X11L and dp1. *F Do you have map locations for X11L and dp1? It is nice if we can keep *F as many gene records as possible anchored to the map. *F You say 'One clone tentatively named dp1..' Have you settled on a *F symbol/name for dp1 yet? *F Is X11L related to Human XLL, now known as APBA1? *F I realise that you are neither the first nor the last author for this *F abstract - however you are the only author whose contact information is *F in FlyBase. Feel free to pass this query on to one of the other *F authors, if that is more appropriate. *F Thank you for your help, *F with best wishes, *F Rachel. *F From yyagi@mayqueen.f.u-tokyo.ac.jp Mon Mar 08 04:10:56 1999 *F To: rd120@gen.cam.ac.uk *F Subject: Re: ADRC-560A *F Dear Dr Drysdale *F I have forwarded your e-mail for Dr Nakamura. *F I'm first author for the abstract. *F I answer your questions. *F dp1 is on 61F. Near Klp61F. We are trying to determine X11L locus but we *F have not *F determined yet. We can determine the X11L locus soon. *F >> You say 'One clone tentatively named dp1..' Have you settled on a *F >> symbol/name for dp1 yet? *F We have not. We do not found known mutation on 61F as dp1 mutant. *F >> *F >> Is X11L realted to Human XLL, now known as APBA1? *F >> *F Following to recent nomenclature, X11L is called APBA2. We used human APBA2 cDNA *F as a probe for screening. dX11L has homology with both APBA1 and 2 in PID *F (PTD) and *F PDZ domain. N terminal region homology is low. We don't have another clone *F and we cannot *F conclude dX11L is APBA1 homolog or APBA2 homolog, or only one homolog gene *F in fly. *F >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> *F Yoshimasa Yagi, PhD. *F Laboratory of Neurobiophysics *F School of Pharmaceutical Sciences *F The University of Tokyo *F Hongo, Bunkyo-ku, Tokyo 113-0033 *F Japan *F TEL +81-3-3812-2111 ext.4803 *F FAX +81-3-3814-6937 *F E-mail yyagi@mayqueen.f.u-tokyo.ac.jp *F <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< # *U FBrf0108093 *a Yokoyama *b H. *t 1999.3.15 *T personal communication to FlyBase *u *F >From k46425a@nucc.cc.nagoya-u.ac.jp Mon Mar 15 10:52:42 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 15 Mar 1999 10:52:42 +0000 *F Date: Mon, 15 Mar 1999 20:00:11 +0900 *F To: ma11@gen.cam.ac.uk *F From: k46425a@nucc.cc.nagoya-u.ac.jp (yokoyama) *F X-Sender: k46425a@nucc.cc.nagoya-u.ac.jp (Unverified) *F Subject: Re: Help FlyBase please *F X-Mailer: Eudora-J(1.3.3J7) *F MIME-Version: 1.0 *F Dear Dr. Ashburner *F I will answer your question. Our prefered symbol of this gene is Kaz1. *F Kaz1 encoded multiple splice variants of Kazal-type serine protease *F inhibitor-like proteins. Kaz1 consists of five exons and four *F alternatively retained intorons to produce six transcripts of type *F AB(AB017699), C1(AB017972), C2(AB017973), C3(AB017974), D(AB017975) and *F E(AB017976). The AB transcript contains two open reading frames of which *F the upstream one produces a polypeptide having a mitochondrial sorting *F signal. The down stream open reading fram of type AB transcript is shared *F partially with type C1, C2, C3, D and E. Type C1, C2, C3 transcripts differ *F only in 5'-noncoding sequence. Type C1, C2, C3 and E transcripts have a *F signal sequence at their amino-termini. While B and D transcripts lack this *F sequence region. *F Sincerely, *F Hiroaki Yokoyama (graduate student) *F Nagoya University Bioscience Center *F Chikusa,Nagoya 464-8601,Japan *F TEL.+81-52-789-5236 *F FAX.+81-52-789-5228 *F e-mail;k46425a@nucc.cc.nagoya-u.ac.jp # *U FBrf0108103 *a Zaffran *b S. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:37:33 1999 *F To: zaffrans@yahoo.com *F Subject: ADRC-702B *F Dear Stephane, *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'Identification and characterization of barbu, a novel E(spl)m4 related *F gene encoding a protein partner of the NK homeodomain protein Tinman.' *F You mention a gene that is new to FlyBase, barbu. *F Do you have a map location for barbu? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From zaffrans@yahoo.com Wed Mar 03 18:24:23 1999 *F Subject: Re: ADRC-702B *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F Like I'll write in my poster about the 'Identification and *F characterization of barbu, a novel E(spl)m4 related gene encoding a *F protein partner of the NK homeodomain protein Tinman.' this new gene *F barbu is located on the third chromosome at 2Kb of Bearded gene, so at *F 71A band. *F I hope that will be enough for you but let me know if you need more *F information. *F Best wishes, *F Stephane # *U FBrf0108109 *a Zhang *b Y. *t 1999.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Mar 03 11:29:33 1999 *F To: yzhang@biology.utah.edu *F Subject: ADRC-572A *F Dear Yong, *F Hi there and how are you doing? *F We are currently curating the abstracts for the upcoming Bellevue *F (Seattle) Annual Drosophila Research Conference, for FlyBase. I am *F writing in connection with your abstract: *F 'The Drosophila eye color mutant ruby encodes 3 subunit of AP3 complex *F and is involved in synaptic transmission.' *F You say that the rb subunit of 'AP3' was identified from an EST. *F Please can you tell me which EST, as these are the kinds of links we *F need to make? *F Thank you for your help, *F with best wishes, *F Rachel. *F From yzhang@biology.utah.edu Wed Mar 03 16:22:27 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-572A *F Dear Rachel *F These two overlapping ESTs are LD08782 and LD22852, and the EMBL entry for *F beta3 subunit of AP3 is AJ011778, plus a related sequence AJ131729, which *F is the distal breakpoint of In(1)rb-D1. The distal breakpoint of In(1)rb-D1 *F disrupts the coding region of beta3 subunit. *F With best wishes, Yong # *U FBrf0108120 *a Hartl *b D. *t 1999.4.13 *T personal communication to FlyBase *u *F Archived # *U FBrf0108121 *a Liqun *b L. *t 1999.3.23 *T personal communication to FlyBase *u *F From a 04:28 PM 3/23/99 letter from Luo: *F *F Also we have confirmed tub-GAL80 insertions at 2L, 3L (thus complete the *F whole MARCM set!). We will send the recombinant chromosomes (with FRT40A *F or FRT80B) to you soon. *F *F From a later letter: *F *F X-Sender: lluo@popserver4.stanford.edu *F Message-Id: *F In-Reply-To: <4.1.19990401141803.0094df00@sunflower.bio.indiana.edu> *F References: *F <4.1.19990322123724.0098ce00@sunflower.bio.indiana.edu> *F Mime-Version: 1.0 *F Content-Type: text/plain; charset="us-ascii" *F Date: Fri, 14 May 1999 18:10:32 -0800 *F To: Kevin Cook *F From: Liqun Luo *F Subject: Re: MARCM stocks *F *F Hi, Kevin, *F *F My technician will send you the three stocks that are recombinant of *F GAL80(2L) and GAL80 (3L) with FRTs next Monday. You guys have saved us *F lots of work of sending out stocks! Thanks very much. *F *F Liqun *F *F =================================================================== *F Liqun Luo, Ph. D. Tel: (650)723-6645 *F Assistant Professor Fax: (650)723-0589 *F Department of Biological Sciences Dept Fax: (650)723-6132 *F Herrin Labs 144A E-mail: lluo@stanford.edu *F 385 Serra Mall *F Stanford University *F Stanford, CA 94305-5020 *F ================================================================ *F # *U FBrf0108122 *a Strutt *b D. *t 1999.5.20 *T personal communication to FlyBase *u *F *F From crosby@morgan.harvard.edu Mon May 24 12:25:27 1999 *F Received: from beadle.harvard.edu (beadle [140.247.91.109]) *F by morgan.harvard.edu with ESMTPœ id MAA25128; *F Mon, 24 May 1999 12:25:26 -0400 (EDT) *F From: Madeline Crosby *F Received: by beadle.harvard.edu with id MAA09907 *F Date: Mon, 24 May 1999 12:26:39 -0400 (EDT) *F Message-Id: <199905241626.MAA09907@beadle.harvard.edu> *F To: joec@morgan.harvard.edu *F Subject: pc from David Strutt *F Cc: crosby@morgan.harvard.edu *F X-Sun-Charset: US-ASCII *F Content-Length: 1772 *F Status: R *F *F *F Date: Thu, 20 May 1999 19:05:31 +0100 (BST) *F From: David Strutt *F X-Sender: mb1dis@stoat *F To: Kevin Cook *F Subject: Re: P{w[+mC]=Ubi-GFP} insertions *F In-Reply-To: <4.1.19990520085110.0095b8e0@sunflower.bio.indiana.edu> *F Message-ID: *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F *F *F With regard to the Ubi-GFP insertions, this is what I know: *F *F The insertion on 3R does indeed map to polytene internal 83. This is a *F jump that I isolated myself from one of Ilan Davis' original lines. I *F told the lucky technician who mapped the jump not to bother defining the *F map position beyond a numbered division. *F *F I'm not so sure about the insertion(s) on 2L. I isolated 1 jump myself at *F 36, and mapped two of Ilan's original lines to 33 and 38. I made *F recombinants of these insertions with FRT40 in various combinations, and *F retained two lines, each with two insertions. One line was 33+38 and the *F other 36+38. And at one time I also had lines with only 1 Ubi-GFP *F insertion on the FRT40 chromosome. *F *F I think I sent either 33+38 FRT40 or 36+38 FRT40 to David Bilder, but I *F don't have any record of this. If he doesn't know either, then the *F information is lost. *F *F I'll happily send you a line which has insertions in known positions! *F *F I also have a line with an insertion on the X recombined with FRT18[neo], *F which is a natural partner to these other lines. I don't know where the *F insertion in this maps though... *F *F I hope this is of help. *F *F Best regards, *F *F David Strutt *F *F ----------------------------------------------------------------------------- *F *F Dr. David Strutt *F Developmental Genetics Programme *F University of Sheffield *F Firth Court *F Western Bank *F Sheffield *F S10 2TN *F England *F *F *F # *U FBrf0108355 *a Whitfield *b E. *c A. *d Tatarenkov *t 1999.4.12 *T personal communication to FlyBase *u *F Received: from ebi.ac.uk (dhcp56.ebi.ac.uk 193.62.196.149) *F by alpha1.ebi.ac.uk (8.8.7/8.8.7) with ESMTP id LAA14227; *F Mon, 29 Mar 1999 11:28:49 +0100 (BST) *F Message-ID: <36FF55EE.AD0D6810@ebi.ac.uk> *F Date: Mon, 29 Mar 1999 11:29:02 +0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: fjayala@uci.edu *F CC: Eleanor Whitfield *F Subject: Help Swiss-prot - Cs protein sequence *F Content-Type: text/plain; charset=us-ascii *F Content-Transfer-Encoding: 7bit *F Dear Dr. Ayala, *F I am an annotator (curator) at the SWISS-PROT protein sequence database *F (http://www.expasy.ch/sprot or http://www.ebi.ac.uk/sprot) *F and I have a question I am hoping you can answer. *F While curating the SWISS-PROT entry G3790084 *F (See *F http://www.expasy.ch/cgi-bin/get-full-entry?TREMBL_NEW-ID:'G3790084') *F I encountered the following problem: *F The sequence you submitted is very different from that submitted by *F Eveleth and Marsh (Molec. General Genet. 209: 290-298 (1987)). They *F suggest there are several potential open reading frames in the Cs *F region, none of which show a strong codon bias. Their sequence is only *F 245 amino acids but does have two regions that are identical to regions *F of your 504 amino acid sequence. *F Do you know the origin of the sequence discrepancies? Alternative *F splicing, frameshifts etc. *F I would be very grateful if you could spare the time to answer this *F question. *F With my best regards, *F Eleanor Whitfield. *F Received: from e4e.oac.uci.edu (antatare@e4e.oac.uci.edu 128.200.222.10) *F by alpha1.ebi.ac.uk (8.8.7/8.8.7) with ESMTP id DAA24695 *F for ; Wed, 31 Mar 1999 03:58:17 +0100 (BST) *F X-Authentication-Warning: e4e.oac.uci.edu: antatare owned process doing -bs *F Date: Tue, 30 Mar 1999 18:58:14 -0800 (PST) *F From: Andrei N TATARENKOV *F X-Sender: antatare@e4e.oac.uci.edu *F To: Eleanor Whitfield *F cc: 'Francisco J. Ayala' *F Subject: Re: Help Swiss-prot - Cs protein sequence *F In-Reply-To: <199903302201.OAA01357@e4e.oac.uci.edu> *F Message-ID: *F MIME-Version: 1.0 *F Content-Type: TEXT/PLAIN; charset=US-ASCII *F Dear Dr. Whitfield, *F Nucleotide sequence of Cs in D. melanogaster submitted by Eveleth and *F Marsh (1987) differs from our sequence (Tatarenkov, Saez, Ayala) by *F occurrence of several deletions and insertions (indels), with consequent *F shifts in reading frame. *F We decided to re-sequence Cs in D. melanogaster for verification purposes *F during our work with Scaptodrosophila lebanonensis, when we found that *F sequences of the two species are highly similar along 1.5 kb region at *F nucleotide level, but only in a few stretches at amino acid level. This *F region is an ORF in S. lebanonensis. Our Cs sequence of D. melanogaster *F differs from the published sequence by the occurrence of indels, which are *F predicted by the alignment of the previously published sequence with the *F Cs sequence of S. lebanonensis. Encoded peptide sequence obtained by *F translating the corrected sequence of Cs in D. melanogaster is 78% *F identical to that of S. lebanonensis. *F We also sequenced Cs in D. simulans, which is a closely related species to *F D. melanogaster. We found that ORF arrangement in D. simulans is identical *F to our sequence of D. melanogaster, but different from previously *F published sequence by Eveleth and Marsh. In both species we found a long *F ORF that extends for 1507 bp from the intron, determined in D. *F melanogaster by Eveleth and Marsh. The longest ORF previously proposed is *F 735 bp. Thus, the coding region of Cs is twice as long, as previously *F thought. *F Results of our study will be published in GENE under the title 'A compact *F gene cluster in Drosophila: the unrelated Cs gene is compressed between *F duplicated amd and Ddc' by A. Tatarenkov, A.G. Saez, and F.J. Ayala. *F Please, let me know if you have any other questions. *F Sincerely yours, Andrei Tatarenkov. *F \---------------------------------------------- *F On Tue, 30 Mar 1999, Francisco J. Ayala wrote: *F > Andrey: *F > *F > Will you please answer this? (Let me have a copy of your reply.) *F > *F > Thanks, *F > *F > Francisco *F > *F > ------------ *F > *F > >X-From_: eleanor@ebi.ac.uk Mon Mar 29 02:29:45 1999 *F > >Date: Mon, 29 Mar 1999 11:29:02 +0100 *F > >From: Eleanor Whitfield *F > >To: fjayala@uci.edu *F > >CC: Eleanor Whitfield *F > >Subject: Help Swiss-prot - Cs protein sequence *F > > *F > >Dear Dr. Ayala, *F > > *F > >I am an annotator (curator) at the SWISS-PROT protein sequence database *F > >(http://www.expasy.ch/sprot or http://www.ebi.ac.uk/sprot) *F > >and I have a question I am hoping you can answer. *F > > *F > >While curating the SWISS-PROT entry G3790084 *F > >(See *F > >http://www.expasy.ch/cgi-bin/get-full-entry?TREMBL_NEW-ID:'G3790084') *F > >I encountered the following problem: *F > > *F > >The sequence you submitted is very different from that submitted by *F > >Eveleth and Marsh (Molec. General Genet. 209: 290-298 (1987)). They *F > >suggest there are several potential open reading frames in the Cs *F > >region, none of which show a strong codon bias. Their sequence is only *F > >245 amino acids but does have two regions that are identical to regions *F > >of your 504 amino acid sequence. *F > >Do you know the origin of the sequence discrepancies? Alternative *F > >splicing, frameshifts etc. *F > > *F > >I would be very grateful if you could spare the time to answer this *F > >question. *F > > *F > >With my best regards, *F > >Eleanor Whitfield. *F > > *F > > *F > > *F > > *F > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F > Francisco J. Ayala *F > Donald Bren Professor of Biological Sciences *F > Department of Ecology and Evolutionary Biology *F > University of California *F > 321 Steinhaus Hall *F > Irvine, CA 92697-2525 *F > tel: 949-824-8293 *F > fax: 949-824-2474 *F > fjayala@uci.edu *F > http://ecoevo.bio.uci.edu/faculty/ayala/index.html *F > http://fpnt.acs.uci.edu/scripts/UCIFacultyProfiles/DetailDept.CFM?ID=2134 *F > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F > *F > *F From eleanor@ebi.ac.uk Wed Mar 31 09:20:28 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 Mar 1999 09:20:28 +0100 *F Date: Wed, 31 Mar 1999 09:22:55 +0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: 'Rachel Drysdale (Genetics)' , *F 'Gillian Millburn (Genetics)' *F CC: Eleanor Whitfield *F Subject: anons needed please. *F Content-Transfer-Encoding: 7bit *F Hi, *F Could you please add anon designations to a couple of lone ORFs shown in *F Fig 1, pg 291 of: *F Eveleth, D.D., Marsh, J.L. *F Overlapping transcription units in Drosophila: sequence and structure of *F the Cs gene. *F Molec. gen. Genet. 1987 209:290--298 *F FBrf0046992 *F I have written to an author of a subsequent paper and he has confirmed *F there are many sequencing errors in this paper, such that 3 of the 5 *F ORFs shown in Fig 1 are actually l(2)37Cs, the remaining 2 are not. *F Those two are the 4th and 5th ORF, if you read them from left to right *F (the remaining three include 2 that show the presence of an intron and *F the third is the longest shown). *F thanks *F Ele *F From gm119@gen.cam.ac.uk Mon Apr 12 14:27:14 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Apr 1999 14:27:14 +0100 *F To: eleanor@ebi.ac.uk *F Subject: Re: anons needed please. *F Cc: gm119@gen.cam.ac.uk, rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 12 Apr 1999 14:30:03 +0100 *F Content-Length: 1382 *F > Hi, *F > *F > Could you please add anon designations to a couple of lone ORFs shown in *F > Fig 1, pg 291 of: *F > Eveleth, D.D., Marsh, J.L. *F > Overlapping transcription units in Drosophila: sequence and structure of *F > the Cs gene. *F > Molec. gen. Genet. 1987 209:290--298 *F > FBrf0046992 *F > *F > I have written to an author of a subsequent paper and he has confirmed *F > there are many sequencing errors in this paper, such that 3 of the 5 *F > ORFs shown in Fig 1 are actually l(2)37Cs, the remaining 2 are not. *F > Those two are the 4th and 5th ORF, if you read them from left to right *F > (the remaining three include 2 that show the presence of an intron *F > and the third is the longest shown). *F OK. here is a picture of Fig1. in the paper to make sure I get this right: *F _/\ |_________________________ *F 1 2 *F _/\__________ |___ *F 3 4 *F |__________ *F 5 *F so I think that you're saying that 1, 2 and 3 are actually l(2)37Cs ? *F while 4 and 5 aren't ? are 4 and 5 actually open reading frames, but *F just not of l(2)37Cs ? *F I will make this a pc from you to FlyBase. *F The anons from Fig1. will be: *F anon-37Cb for transcript 5 *F and *F anon-37Cc for transcript 4 *F (unless I got them wrong and 4 and 5 aren't the anon ORFs) *F Gillian *F From eleanor@ebi.ac.uk Mon Apr 12 14:56:48 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Apr 1999 14:56:48 +0100 *F Date: Mon, 12 Apr 1999 15:00:19 +0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: 'Gillian Millburn (Genetics)' *F CC: rd120@gen.cam.ac.uk, Eleanor Whitfield *F Subject: Re: anons needed please. *F Content-Transfer-Encoding: 7bit *F >so I think that you're saying that 1, 2 and 3 are actually l(2)37Cs ? *F indeed *F >while 4 and 5 aren't ? are 4 and 5 actually open reading frames, but *F >just not of l(2)37Cs ? *F indeed *F >I will make this a pc from you to FlyBase. *F Well strictly it is information provided from Andrei Tatarenkov to me, *F is there anyway to represent that? *F I can forward you the emails if you wish. *F thanks again *F Ele # *U FBrf0108496 *a Timakov *b B. *t 1999.4.23 *T personal communication to FlyBase *u *F >From bet94001@uconnvm.uconn.edu Fri Apr 23 17:08:57 1999 *F Date: Fri, 23 Apr 1999 12:18:32 -0400 *F From: BEN TIMAKOV *F To: flybase-help@morgan.harvard.edu *F Hello fellow colleagues, *F The following has come to our attention. While searching the database *F with poe sequence, retrived via the flybase, the result was unexpected. *F It turns out that the available partial poe sequence appears to be *F identical to the pushover sequence, even at the animoacid level search. *F Are they the same gene or is there another answer? By the way, pushover *F is completely sequenced and has recently been posted on the net. *F Please reply, *F Ben Timakov..... *F > Dear Ben *F > *F > Thanks for your email. poe and pushover map to about the same place *F > (28E1 is what FlyBase says for pushover and 28E1-9 for poe). FlyBase *F > has a sequence for pushover, but not for poe, so I cannot confirm *F > that they are the same. Where did you get the poe sequence from ? *F > *F > If they are the same then we need to find out which name was used *F > first - that will be the valid name. Rachel Drysdale will look *F > into this and advise. *F > *F > Michael Ashburner. *F >From bet94001@uconnvm.uconn.edu Mon Apr 26 14:55:29 1999 *F Date: Mon, 26 Apr 1999 10:05:13 -0400 *F From: BEN TIMAKOV *F To: 'Michael Ashburner (Genetics)' *F Subject: poe & pushover *F Dera Michael *F Thank you for the reply. Yes, after noticing their map positions I was really inclined to write *F about this issue. Here is the accession number for ms(2)01659, a Drosophila melanogaster P lethal *F line : AQ026403. Also, ms(2)01659 is synonymus with poe, induced by P-element mutagenesis. I hope *F this helps out and I would like to know the outcome, if possible. *F Ben Timakov....... # *U FBrf0108497 *a Maroy *b P. *t 1999.4.20 *T personal communication to FlyBase *u *F >From maroy@sol.cc.u-szeged.hu Tue Apr 20 11:27:07 1999 *F To: Michael Ashburner (Genetics) *F Subject: Re: Help FlyBase please *F Dear Michael, *F I am sorry for the delay, but I was away. *F We just finished a manuscript regarding to the 70CD region. I think the best *F to answer to copy the appropriate sections following your questions. *F > *F >FB knows about Hsc70C-1 at 70C. Is 'hsc70Cb' a second Hsc70 in this region ? *F Yes. *F > *F > *F >Do I deduce from this that: *F >l(3)02402, l(3)s4868, l(3)084807 are alleles of the same gene ? If so *F > what should that gene.s name be ? *F I think it should be l(3)70Da. *F 'l(3)70Da may code for a helicase: All known mutations in the l(3)70Da gene *F (cytological location: 70D1) are only semi-lethals; they all give adult *F escapers, even over deficiencies, suggesting that this gene plays an almost *F dispensable role in development (A. T. C. Carpenter, pers. comm.). Moreover, *F homozygous third instar larvae show necrosis in salivary gland cells. *F Plasmid rescue fragment sequences show that the insertion in l(3)S084807 is *F 15 bp distal to the insertion site of l(3)02402 and 107 bp distal to *F l(3)s4868, which has been sequenced by the BDGP. A transcribed region, which *F may correspond to l(3)70Da, was identified by Klämbt, Glazer and Shilo *F (1992) less than 1 kb proximal to these insertions (see Fig. 3). Moreover, *F sequences distal to the plasmid rescue site of l(3)02402 (see Fig. 3) reveal *F an ORF of 135 amino acids, which displays low but significant similarities *F to the amino-terminal region of the yeast helicase RAD26 (Acc. no. L26910).' *F > *F >l(3)027714, l(3)j2E11, are alleles of the same gene ? Is 027714 = S027714 ? *F yes, 027714 = l(3)S027714 *F 'l(3)S027714: The l(3)S027714 insertion is L1 lethal. The sequences adjacent *F to its insertion site display similarities to those recovered from the 3' *F end of the lethal insertion l(3)j2E11 (Bier et al. 1989) and to the ESTs *F GH18550, GH21710 and GH21824, which all start 396 bp downstream of the *F l(3)S027714 insertion point. However, l(3)S027714 complements l(3)j2E11. The *F combined sequences indicate an ORF of 180+ amino acids, beginning at bp 454 *F and still open at the 3' end. BLASTX searches show that the putative gene *F product of l(3)S027714 is highly similar to Xenopus XPMC2 (Acc. No. U10185), *F a cell cycle gene that can complement mitotic catastrophe mutations in yeast *F (Su and Maller 1995), and to a hypothetical exonuclease YOL080c of *F Saccharomyces cerevisiae (Acc. no. Z74822). *F According to the Southern blots using the plasmid rescue fragment as a *F probe, the insertion on the l(3)S027714 chromosome is located between the *F l(3)70Da and l(3)70Ca/b loci. However, this result is in conflict with 1) *F the complementation of its lethal mutation with Df(3L)D-5rv6 (Fig. 1), 2) *F the position of the l(3)j2E11 insertion in 70C5-6 (BDGP), and 3) Southern *F blot experiments using genomic DNA (data not shown). It is, therefore, *F likely that the P-element insertion site and lethality do not overlap. We *F must consider the possibility that the Southern blot experiments were *F complicated by unrecognized cross-hybridizations and, therefore, we regard *F the molecular position of l(3)S027714 as uncertain.' *F > *F >l(3)134217, l(3)00082, l(3)07621 are all alleles of l(3)70Ca ? *F >Is 134217 = S134217 ? *F yes, 134217 = l(3)S134217 *F Here we have a complex situation. The P insertion mutants belonging to 70Ca *F and 70Cb complementation groups are not complementing with the members of *F the other groups, but the molecular data suggest, that they are disrupting *F the same transcriptional unite *F 'A novel hsp70 homologue: We determined the full sequence of the plasmid *F rescue clone deriving from l(3)S148513 (Acc. no. AJ132829). It measures 7728 *F bp and comprises the complete coding region of a novel hsp gene, which is *F located proximal to l(3)S148513 insertion site (Fig. 5). The sequence data *F indicate that, although we observed full complementation between mutations *F in the l(3)70Ca (Carpenter 1994) and l(3)70Cb (Karpen and Spradling 1992; *F Carpenter 1994) loci, these sites very likely affect the same coding region. *F The l(3)70Cb allele l(3)S134217 carries a P element inserted 1246 bp *F proximal to l(3)S148513, a mutation that fails to complement l(3)70Ca1 *F (Carpenter 1994). l(3)S148513 is likely inserted in the 5' control region of *F this gene, while all three known P elements defining the l(3)70Cb *F complementation group are located in the second intron: l(3)S134217 is *F inserted at bp1246, just 3 bp proximal to l(3)00082 (STS Dm0089), and 276 bp *F distal to l(3)07621 (bp 1522) (see Fig. 5). l(3)S134217 and l(3)S148513 *F exhibit similar lacZ staining patterns (Table 4), consistent with their *F transgenes being driven by the same enhancer. It is conceivable that either *F trans-splicing of the nascent mRNA molecules or the differential use of *F promoters is responsible for this rather surprising pattern of *F complementation. However, from our data we cannot exclude the possibility *F that l(3)S148513 is a non-lethal insertion that carries an additional *F mutation responsible for its lethality over l(3)70Ca1. At the 3' end (bp *F 5523 to 5846), the plasmid rescue clone overlaps with the distal end of the *F P1 phage DS01408, which gave rise to STS Dm2566. This confirms the *F localization and the orientation of this gene independent of the Southern *F blotting experiments (Fig. 2). *F Similarity searches show that this sequence is for long stretches almost *F identical to the EST defined by the clot 1489 (BDGP, unpublished). The P *F element of l(3)S148513 is inserted 47 bp upstream of the 5' end of the EST *F clot 1489 consensus, and 15 bp in front of the longest EST (GH12450). Since *F the ESTs correspond to cDNA, the exon-intron structure of the gene can be *F determined. Within the sequenced part, five introns of 341 bp (bp 89/114 to *F 454/457), 1316 bp (bp 728 to bp 2050), 64 bp (bp 3003 to 3066), 1573 bp (bp *F 3598 to 5169) and 88 bp (bp 5370 to 5457) were identified. The first exon *F does not code for any amino acids, but there are two alternative splice *F sites for the first intron. *F The coding region comprises 2412 bp that can be translated into 804 amino *F acids. It starts in the second exon at bp 621 and stops at bp 6078. There is *F a polyadenylation site present at bp 6241. The resulting polypeptide has a *F deduced molecular mass of 88.6 kDa. Database searches using BLASTX or *F TBLASTN show that this ORF displays strong similarities to members of the *F mammalian HSP70/110 family. We therefore named the new hsp gene tentatively *F hsc70Cb (heatshock protein cognate 70Cb). The highest similarity score was *F obtained with the rat ischemia responsive 94 kDa protein (Acc. no. *F AF077354). The l(3)70Ca/b sequence is not particularly related to the HSP70 *F cognate hsc70A1 (Rubin et al. 1993), which is also located in 70C (Craig, *F Ingolia and Manseau 1983); it is therefore very likely that there are at *F least two HSP70 homologues in this region. Both l(3)S148513 and l(3)S134217 *F are embryonic lethal as homozygotes, indicating an essential role of this *F gene in Drosophila development. A universal role of the gene product in the *F living animal is also supported by the observation that the ESTs derive from *F cDNA libraries of different stages and tissues.' *F > *F >What is the relationship between l(3)70Ca and 'hsc70Cb' and or Hsc70C-1 ? *F I think, the text above answers this, l(3)70Ca identifies 'hsc70Cb', and the *F sequence of hsc70Cb is different to Hsc70C-1. *F > *F I admit, the whole picture is complex. But this is what we have. *F With the best regards *F Peter *F Peter Maroy, Deptartment of Genetics and Molecular Biology *F A.Jozsef University, Kozepfasor 52., H-6726 Szeged, Hungary *F email: MAROY@SOL.CC.U-SZEGED.HU *F tel:36-62-454025, fax:36-62-432485 # *U FBrf0108499 *a Scholey *b J. *t 1999.5.5 *T personal communication to FlyBase *u *F From jmscholey@ucdavis.edu Wed May 05 23:24:17 1999 *F To: flybase-help@morgan.harvard.edu *F I wanted to point out that flybase contains references to both KRP130 and *F KLP61F. In fact, KRP130 is almost certainly the protein holoenzyme produced *F from the KLP61F gene (Kashina et al, 1996). We no longer refer to KRP130 *F (using KLP61F instead) and it would probably simplify things if this were *F also done in flybase and if information on KRP130 was combined with that on *F KLP61F in flybase as well. In addition we have two new papers on KLP61F *F (Sharp et al, 1999, J. Cell Biol, 144;125 and Sharp et al, 1999 Nature Cell *F Biology, 1, 51-54). Hope this is useful. *F Sincerely, *F Jonathan Scholey. # *U FBrf0108500 *a Moore *b D. *t 1999.4.29 *T personal communication to FlyBase *u *F Archived. # *U FBrf0108503 *a Birchler *b J. *t 1999.5.10 *T personal communication to FlyBase *u *F >From BirchlerJ@missouri.edu Mon May 10 18:43:22 1999 *F To: Michael Ashburner (Genetics) *F Subject: Re: Help FlyBase please *F >Jim *F > *F >Thanks. Yes I had a reply from Lisa, which was not very clear. *F > *F >My interpretation is that there are four genes on this sequence - as judged *F >by coding content: *F > *F >27..963 Nac&agr; *F >1138..2911 Dgk&egr; *F >3189..3519 Ucr9 *F ><4457..>4720 tafazzins *F > *F > *F >There is also the P-element associated with oxen1 at 3147 *F > *F >The confusing thing is that the record says: *F > *F > mRNA 27..963 *F > /gene='oxen' *F > /product='alpha NAC' *F > gene 27..963 *F > /gene='oxen' *F > *F > *F >which I take to mean that the product of oxen is Nac&agr; and I assume *F >you have some other evidence for this, given the fact that the ox1 P *F >insertion is between the 5' ends of Dgk&egr; and Ucr9. *F > *F >Correct ? *F > *F >Michael *F Michael: *F OK, let me see if I can explain the sequence of events. The first *F entry to Genbank several years ago reported the aNAC sequence. It was *F identified by rescue of the ox1 P element and probing a cDNA library with *F the surrounding sequences. The level of aNAC RNA is reduced in the ox1 *F mutation, but not in the revertant. For that reason, oxen was included in *F the gene entry. *F Further work over the years has revealed a morass of closely packed *F genes in the region. These data suggest that the primary lesion of oxen is *F not an effect on aNAC, although given the dense and overlapping *F organization of this region, it is possible that the lesion affects several *F genes. The updated entry to Genbank included a request to remove the gene *F entry as being oxen, but this did not occur. We have sent another *F communication making this request again. *F The ox1 mutation affects the RNA levels of aNAC, Dgk and Qcr9. *F Transformants of aNAC do not rescue the mutant phenotype of ox1. *F Transformants of Dgk do not rescue the mutant phenotype of ox1. *F Transformants of Qcr9 are in progress, so we don't know the answer yet. *F (Note that the orthologous gene in yeast that you abbreviated as Ucr9 is *F referred to as QCR9.) *F The bottom line is that we do not know definitively which of these *F genes corresponds to the ox1 mutation. It is even possible that the ox1 *F mutation affects several genes in this region and that the mutant phenotype *F is a combination effect. I believe that the best course of action is to *F hold in abeyance the assignment of a gene to the ox1 mutation. Can *F Flybase deal with that ambiguity for the time being? *F Let me know if you need further information. *F Regards, *F Jim *F James A. Birchler *F Professor of Biological Sciences *F University of Missouri *F 117 Tucker Hall *F Columbia, MO 65211 *F (573) 882-4905 (voice) *F (573) 882-0123 (FAX) # *U FBrf0108507 *a Harrison *b S. *t 1999.5.12 *T personal communication to FlyBase *u *F Archived. # *U FBrf0108508 *a Caggese *b C. *t 1999.5.14 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri May 14 16:20:12 1999 *F To: caggese@bioserver.uniba.it *F Subject: Helping FlyBase: Tctex *F Dear Dr. Caggese, *F We have been curating the DNA sequence records for FlyBase and have a *F question for you. You have recently submitted a sequence record, *F accession number AF123058; AAD30033 for the Tctex gene, which you say *F encodes a dynein light chain and maps to 90F9--91A2. We already have a *F record for a dynein light chain gene at 90F, and I was wondering *F whether you knew if this was the same gene as yours? I enclose what we *F have about the other gene below, for you to see. I will be querying *F the authors of the abstract to see if they can add anything more. *F \*a Dlc90F *F \*z FBgn0024432 *F \*c 90F *F \*c Left limit from (method unavailable) (FBrf0101355) *F \*c Right limit from (method unavailable) (FBrf0101355) *F \*x FBrf0101355 == Li et al., 1998, A. Conf. Dros. Res. 39: 318A *F \*E FBrf0101355 == Li et al., 1998, A. Conf. Dros. Res. 39: 318A *F \*e Dynein light chain 90F *F \*c 90F *F \*d dynein light chain *F \*p Lethal lines have been obtained by imprecise excision of a @P-element@ *F \*p inserted 800bp upstream of the transcription start site of @Dlc90F@. *F \*u Southern blot analysis indicates that @Dlc90F@ is encoded by a single *F \*u copy gene and is expressed as a single transcript throughout Drosophila *F \*u development. Western blot experiments show that the 14kD light chain is *F \*u co-purified with cytoplasmic dynein from embryos and there exist two *F \*u isoforms, suggesting a post-translational regulation of the light chain. *F Also, you annotated your sequence with three intriguing gene symbols *F > gene 1672..2476 *F > /note='EP(3)3634, l(3)04091, l(3)05089' *F This implies to me that you know that the flanking sequence of *F insertions in genome project lines EP(3)3634, l(3)04091 and l(3)05089 *F matches sequence corresponding to Tctex. This is of relevance because *F we (FlyBase) already have a gene entry for l(3)04091, with an allele *F l(3)0409104091. If that maps to Tctex I should merge the records *F for l(3)04091 and Tctex (keeping Tctex as the valid symbol) and record *F an allele Tctex04091 to correspond to what we have now as *F l(3)0409104091. Similarly, if the P insertion in line l(3)05089 maps *F to the Tctex gene, I need to record an allele Tctex05089 (we already *F have and allele of quag, quag05089, at 94A8--B5, but we know that the *F quag phenotype in the line is separable from the P insertion). Finally, if *F EP(3)3634 has an insertion in/affecting the Tctex transcription unit I *F would record it as an an allele of Tctex, Tctex3634. *F So, if you could give me this little bit more information, we would be *F most grateful. *F With best wishes, *F Rachel. *F From caggese@biologia.uniba.it Fri May 14 19:35:13 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: Tctex *F Dear Rachel, *F I isolated a cDNA encoding the putative Drosophila tctex-1 gene at 91F9-11 *F by a strategy for identification of nuclear genes encoding mitochondrial *F protein (accession number Y08968; Caggese et al.(1999). Mol. Gen. Genet. *F 261: 64-70). *F Developmental Northern blot analysis reveals a 0.85-kb transcript in all *F stages examined. This transcript is also present at high levels in the *F oocyte nurse cells. *F Some overlapping genomic clones were isolated and a subclone of 3115 *F nucleotides that contained the entire tctex-1 gene was sequenced (accession *F number AF123058). Sequence analysis revealed that no introns interrupt the *F tctex-1 cDNA sequence . *F A search in the GenBank and Expressed Sequence Tag database using genomic *F nucleotide sequences revealed that two unidentified ESTs (LD12970, *F accession numbers AA438652; CK01205, accession number AA141098) are also *F transcribed from the genomic subclone. *F EST LD12970 (clot 3142): 1262...540 exon; 539...1.. intron *F tctex: 1672...2176 *F EST CK01205: ..3115...2662 *F (the numbers correspond to positions of the genomic subclone) *F I received many available single P-element insertion mutants mapping at *F 90F-91A from Bloomington, Berkeley and Szeged stock collections. In order *F to identify mutant alleles of the tctex-1 gene we used PCR between *F divergent primers derived from the tctex-1 nucleotide sequence and a primer *F derived from the 31-bp terminal inverted repeat sequence of the P element. *F A specific amplification product was detected in the genomic DNA from: *F l(3)05822 single P-element insertion mutant mapping at 90F9-10. Sequence *F analysis revealed that a PZ element is present in this mutant stock at *F position 756-757 of the genomic clone and disrupts the EST sequence LD12970. *F EP(3)3634 single P-element insertion mutant mapping at 91F9-11;91A1. *F Sequence analysis revealed that a EP element is present in this mutant *F stock at position 1671-1672 of the genomic clone and disrupts the tctex-1 *F transcription unit. *F l(3)04091 single P-element insertion mutant mapping at 91F9-11; 91A1. *F Sequence analysis revealed that a PZ element is present in this mutant *F stock at position 1710-1711 of the genomic clone and disrupts the tctex-1 *F transcription unit. *F l(3)05089 single P-element insertion mutant mapping at 91F9-11; 91A1. *F Sequence analysis revealed that a PZ element is present in this mutant *F stock at position 1714-1715 of the genomic clone and disrupts the tctex-1 *F transcription unit. *F BLAST analysis of tctex-1 cDNA sequence showed that 35 nucleotides in the *F UTR are identical to the 35 nucleotides 5' flanking sequence of P element *F in the ms(3)05090 (sdl-2, seedless; Castrillon et al. (1993) Genetics *F 135:489-505) reported as single insertion line at 84F1-84F16 region. *F In reality, sdl-2 is a allele of tctex-1 at 91F9-11; 91A1 since specific *F amplification products were detected in the genomic DNA of sdl-2 mutant *F flies by using different primers derived from the tctex-1 nucleotide *F sequence and a primer derived from the 31-bp terminal inverted repeat *F sequence of the P element. Sequence analysis confirmed that in this mutant *F allele a P element disrupts the dtctex-1 transcription unit by insertion at *F positions 1721-22 of the genomic clone. *F I don't know if multiple inserts are present in this line, but a P element *F is certainly inserted in the tctex-1 gene at nucleotide position which is *F reported in Berkeley Fly Database. *F The phenotypic analysis of the EP(3)3634, l(3)04091, l(3)05089 and sdl-2 *F (ms(3)05090) mutations showed that tctex-1 is required for production of *F functional sperms. *F The manuscript entitled 'The Drosophila melanogaster homolog of tctex-1, a *F putative murine t-complex distorter encoding a dynein light chain, is *F required for production of functional sperm' will be submitted for *F publication as soon as possible. *F With best wishes, *F Corrado *F Prof. Corrado Caggese *F Istituto di Genetica *F Universita di Bari *F via Amendola 165/A *F 70126 Bari, Italia *F tel.+39 080 5443393 *F fax +39 080 5443386 *F e-mail caggese@biologia.uniba.it # *U FBrf0108509 *a Bloomington Drosophila Stock Center *b ?. *t 1999.5.18 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 18 19:54:02 1999 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 18 May 1999 19:54:02 +0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Tue, 18 May 1999 13:54:59 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: masP *F Mime-Version: 1.0 *F Hi folks-- *F Here's a little info to polish up the masP entry (FBal0044170), the *F P{white-un1} entry (FBtp0000017) and the P{wF}3 entry (FBti0001609). *F The isolation of masP is described in Levis et al. (FBrf0042436). It is *F listed in Table 1 as the P(w+ ry+)F insertion at 64B. In the *F Bloomington collection the stock is currently listed as P820 w1118; *F P{w+mF ry+t7.2=wF}3/TM3 (though the genotype needs to be updated). *F This info is also in Murugasu-Oei et al. (FBrf0089757). masP *F (FBal0044170) is the same as P{wF}3 (FBti0001609). *F In FBtp0000017, masP is listed as P{white-un1}masP. I think it should *F be P{w+mF ry+t7.2=wF}masP. I'm not sure how masP got listed as a *F P{white-un1} insertion. P{white-un1} isn't a synonym for P{w+mF *F ry+t7.2=wF} is it? *F Best regards, *F Kevin *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0108511 *a Maines *b J. *t 1999.5.19 *T personal communication to FlyBase *u *F >Date: Wed, 19 May 1999 11:56:04 -0500 (CDT) *F >From: 'Jean Maines' *F >Reply-To: 'Jean Maines' *F >To: kcook@bio.indiana.edu *F > *F >We recently obtaining the stock B-#P648 from your collection, reported to *F >carry *F >a p induced allele of bunched (y1 w67c23; P{w+mC=lacW}bunk06909/CyO). In the *F >course of our work, we discovered that the Berkeley site had information *F >suggesting that this was not an allele of bunched. According to the Berkeley *F >site, this allele, l(2)k06909 is complemented by a bunched allele, l(2)0255. *F >l(2)0255 fails to complement a number of other bunched alleles, suggesting *F >that *F >it is a true bunched allele. Furthermore, the sequence obtained by plasmid *F >rescue from l(2)k06909 demonstrates that the p lies within a neighboring *F gene, *F >elf-1, an elongation factor. While I am not certain where the initial *F >designation of l(2)k06909 as a bunched allele was made, these data suggest *F >that *F >it is, in fact, more likely to be an allele of elf-1. *F > *F >Thank you for your attention to this matter, *F > *F >Jean Maines *F > *F > *F >Jean Z. Maines *F > University of California San Diego *F > Center for Molecular Genetics 312 *F > 9500 Gilman Drive *F > La Jolla CA 92093-0634 *F > (619) 822-2409 *F > (619) 534-7073 (FAX) *F > jmaines@biomail.ucsd.edu *F > *F > or *F > *F > University of Texas Southwestern Medical Center *F > Department of Molecular Biology and Oncology *F > 5323 Harry Hines Blvd NA5-214 *F > Dallas TX 75235-9148 *F > (214) 648-4946 *F > (216) 648-1488 (FAX) # *U FBrf0108516 *a Mount *b S. *t 1999.5.23 *T personal communication to FlyBase *u *F From smount@wam.umd.edu Sun May 23 22:14:12 1999 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: 'snRNP70K' *F Rachel *F Sorry to be slow late in replying. I have had a chance to check the Kim *F and Baker reference. My specific answers follow. *F > We have these two snRNP records which I think must represent the same *F > gene. Could you confirm this for me please (you know us, we need *F > everything in writing!)? *F Yes, these are, without question, the same gene. *F The Kim and Baker reference (FBgn0016978) cites Mancebo et al. *F (FBgn0003458); it doesn't even report any new information. Yannoni and *F White reported results obtained using our antibody, so this is also a *F reference back to Mancebo et al. I believe that Paterson et al. describe *F the protein itself, and the connection to the gene is, once again, *F immunological. There is no evidence of any other gene for a U1 70K *F protein. In summary, the snRNP70K = snRNP27D = U1-70K *F The name we have used, and use informally, is 70K. The name snRNP70K is *F acceptable, and is far preferable to snRNP27D. It also allows us to use *F 70K informally without confusion. So, I'd like to recommend that when you *F fuse them you chose snRNP70K. *F Finally, YES l(2)0210702107 is the same thing as 70K1. Exactly. We *F stopped calling it 2107 and started calling it 70K1 after we showed that *F it could be rescued by 70K produced from a transgene. *F I hope this helps. It was a pleasure meeting you. *F Steve Mount *F \################################################ *F > *F > *F > \*a snRNP70K *F > \*z FBgn0016978 *F > \*x FBrf0059040 == Kim and Baker, 1993, Molec. Cell. Biol. 13(1): 174--183 *F > \*x FBrf0054858 == Paterson et al., 1991, Nucleic Acids Res. 19: 5877--5882 *F > \*x FBrf0093796 == Yannoni and White, 1997, Chromosoma 105(6): 332--341 *F > \*E FBrf0059040 == Kim and Baker, 1993, Molec. Cell. Biol. 13(1): 174--183 *F > \*p A sequence comparison and numerical analysis of the RRM-containing *F > \*p (RNA recognition motif) proteins suggests that functionally related *F > \*p RRM-containing proteins have significant sequence similarities in their *F > \*p RRMs. *F > \*E FBrf0093796 == Yannoni and White, 1997, Chromosoma 105(6): 332--341 *F > \*i snRNP *F > \# *F > \*a snRNP27D *F > \*e small nuclear ribonucleoprotein at 27D *F > \*z FBgn0003458 *F > \*b 2-21 *F > \*c 27D1--2 *F > \*c Left limit from in situ hybridisation (FBrf0052549) *F > \*c Right limit from in situ hybridisation (FBrf0052549) *F > \*g AA201872; BFD:LD03939; dbEST:840982 *F > \*g M31162; AAA28859 *F > \*g Z50206; dbSTS:24170 *F > \*m PIR:A36311 *F > \*m SWP:P17133 *F > \*J PS00030 == Eukaryotic putative RNA-binding region RNP-1 signature. *F > \*d RNA-binding-protein *F > \*d sn-ribonucleoprotein *F > \*x FBrf0064457 == Fukami-Kobayashi et al., 1993, FEBS Lett. 335(2): 289--293 *F > \*x FBrf0100572 == Henriksson and Pettersson, 1997, J. Autoimmun. 10(6): 559--568 *F > \*x FBrf0052549 == Mancebo et al., 1990, Molec. Cell. Biol. 10: 2492--2502 *F > \*x FBrf0054858 == Paterson et al., 1991, Nucleic Acids Res. 19: 5877--5882 *F > \*x FBrf0101502 == Tseng et al., 1998, A. Conf. Dros. Res. 39: 465C *F > \*x FBrf0054695 == Voelker et al., 1991, Molec. Cell. Biol. 11: 894--905 *F > \*x FBrf0093796 == Yannoni and White, 1997, Chromosoma 105(6): 332--341 *F > \*E FBrf0052549 == Mancebo et al., 1990, Molec. Cell. Biol. 10: 2492--2502 *F > \*i U1 70K *F > \*c 27D1--27D2 (determined by in situ hybridisation) *F > \*p @snRNP27D@ has been cloned and sequenced. *F > \*u Isolated from a genomic library using a human U1 70K snRNP cDNA clone as *F > \*u a probe. *F > \*E FBrf0054695 == Voelker et al., 1991, Molec. Cell. Biol. 11: 894--905 *F > \*i U1snRNP-70K *F > \*E FBrf0064457 == Fukami-Kobayashi et al., 1993, FEBS Lett. 335(2): 289--293 *F > \*i U1-70K *F > \*E FBrf0093796 == Yannoni and White, 1997, Chromosoma 105(6): 332--341 *F > \*i snRNP *F > \*E FBrf0054858 == Paterson et al., 1991, Nucleic Acids Res. 19: 5877--5882 *F > \*i 70K *F > \*E FBrf0101502 == Tseng et al., 1998, A. Conf. Dros. Res. 39: 465C *F > \*i U1 70K snRNP *F > \*A snRNP27D1 *F > \*z FBal0086333 *F > \*x FBrf0101502 == Tseng et al., 1998, A. Conf. Dros. Res. 39: 465C *F > \*E FBrf0101502 == Tseng et al., 1998, A. Conf. Dros. Res. 39: 465C *F > \*i 70K1 *F > \*o P-element activity *F > \*G P{}snRNP27D1 *F > \*k Phenotypic class: lethal | recessive *F > \*s @P-element@ insertion in the 5' untranslated region. *F > \*u Excision of the @P-element@ restores viability, as do transgenes *F > \*u carrying either a wildtype genomic fragment or cDNA under the control of *F > \*u the Hsp70 promoter. Some partial revertants show partial female-specific *F > \*u lethality and defects in oogenesis. A transgene construct lacking the *F > \*u arginine-rich domain also rescues the lethality. *F > \# *F > *F > You told me in Bellevue that l(2)02107 corresponds to snRNP27D (by hit *F > of flanking sequence for P{PZ} insert in l(2)0210702107 to snRNP27D *F > sequence). I will merge the record for l(2)02107 with that of *F > snRNP27D. Is '70K1' of FBrf0101502 the same allele as *F > l(2)0210702107 from the BDGP? Looks to be a distinct possibility. *F > *F > Many thanks for your help. I will curate your answer as a personal *F > communication from you to FlyBase. *F > *F > Best wishes, *F > *F > Rachel. # *U FBrf0108518 *a Bloomington Drosophila Stock Center *b ?. *t 1999.5.24 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: TM2 variant *F Date: 24 May 1999 *F *F Information communicated: *F *F TM2, ry[*] to us from John Merrian *F *F # *U FBrf0108522 *a Price *b J. *t 1999.5.25 *T personal communication to FlyBase *u *F Archived. # *U FBrf0108525 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.2 *T personal communication to FlyBase *u *F *************************************************************** *F *F Bloomington Stock Center records, June 1999. *F Second, fourth, and Y chromosome insertions (subset). *F *F >>> *F B-#P121 P{ry[+t7.2]=ry11}RY.1, Dp(1;Y)y[+]/C(1)*, y[1] v[1] f[1]; ry[506] *F Chromosome(s) affected: Y *F Insertion site: Y *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: JSK #10 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P122 P{ry[+t7.2]=ry11}RY.4, Dp(1;Y)y[+]/C(1)*, y[1] v[1] f[1]; ry[506] *F Chromosome(s) affected: Y *F Insertion site: Y *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: JSK #11 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P191 y[1] ac[1]; P{y[+t7.7] ry[+t7.2]=Car20y}25F; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 025F *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Victor Corces *F Comments: y and ac alleles not specified *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P193 y[1] ac[1]; P{y[+t7.7] ry[+t7.2]=Car20y}48?; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 048? *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Victor Corces *F Comments: mapping from E. H. Grell, site is 2-63.5 (8.7 mu to the right of Bl) *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P208 P{ry[+t7.2]=HBDelta-89}52B, Adh[fn6] cn[1]; ry[502] *F Chromosome(s) affected: 2 *F Insertion site: 052B *F Also known as: #JL208 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P210 P{ry[+t7.2]=HBDelta-89}31B Adh[fn6] cn[1]; ry[502] *F Chromosome(s) affected: 2 *F Insertion site: 031B *F Also known as: #JL210 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P221 P{ry[+t7.2]=Hsp70B+65}23A, Adh[fn6] cn[1]; ry[502] *F Chromosome(s) affected: 2 *F Insertion site: 023A *F Also known as: #JL221 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P322 P{ry[+t8.1]=ry1}R304.1; ry[42] *F Chromosome(s) affected: 2 *F Insertion site: 043C *F Also known as: #AS322 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P323 P{ry[+t8.1]=ry1}R305.1; ry[42] *F Chromosome(s) affected: 2 *F Insertion site: 042E *F Also known as: #AS323 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P324 P{ry[+t8.1]=ry1}R306.1; ry[42] *F Chromosome(s) affected: 2 *F Insertion site: 050B *F Also known as: #AS324 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P330 ry[42]; P{ry[+t8.1]=ry3}R401.1 *F Chromosome(s) affected: 4 *F Insertion site: 101h *F Also known as: #AS330 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P368 P{ry[+t7.2]=S6.9}9/CyO; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 052F *F Also known as: #AS368 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: semilethal *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P409 P{ry[+t7.2]=ry11}R704.1; ry[42] *F Chromosome(s) affected: 2 *F Insertion site: 043A, +? *F Also known as: #AS409 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P424 P{ry[+t8.1]=BS2.7B}11/CyO; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 028A *F Also known as: #AS424 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: semisterile *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >> *F B-#P426 P{ry[+t8.1]=BS2.7B}13/CyO; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 032B-C *F Also known as: #AS426 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: semilethal *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P512 y[1] w[67c23]; P{w[+mC]=lacW}k02501, l(2)k02501[k02501]/CyO *F Chromosome(s) affected: 2 *F Insertion site: 038D01-02 *F Date added: 10/16/97 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Istvan Kiss *F Comments: unverified (P{} may or may not cause the lethal), B.D.G.P. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P550 w[1118] sev[14]; P{w[+mW.hs]=sev3}218 *F Chromosome(s) affected: 2 *F Insertion site: 024C *F Also known as: T sev-Met2242.218 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #30; ATP binding site mutant (M3) in genomic sev; eye-color: *F red *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P585 P{ry[+t7.2]=lArB}58, l(2)**/CyO; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 049D *F Also known as: ET 58 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #179; expression pattern like sca according to E.H., but it *F complements sca1, K.M. 2/6/95 *F Larva-Hafen: eye disc, clusters behind the morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P636 w[1118] sev[14]; P{w[+mW.hs]=sev4}SC.1 *F Chromosome(s) affected: 2 *F Insertion site: 050B *F Also known as: Tsev-SC.1 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #201, 2xsev enhancer driving sev+ cDNA; mapped and *F characterized by Bea Christen (Diplomarbeit 90) *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P639 w[1118] sev[14]; P{w[+mW.hs]=sev4}SC.2 *F Chromosome(s) affected: 2 *F Insertion site: 042A-B *F Also known as: Tsev-SC.2 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #202, 2xsev enhancer driving sev+ cDNA; mapped and *F characterized by Bea Christen (Diplomarbeit 90). white-eyed flies *F seen in stock; isolated pure-breeding w+ culture 3/98 KC. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P640 w[1118] sev[14]; P{w[+mW.hs]=sev4}SC.4 *F Chromosome(s) affected: 2 *F Insertion site: 053DE *F Also known as: Tsev-SC.4 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #203, 2xsev enhancer driving sev+ cDNA; mapped and *F characterized by Bea Christen (Diplomarbeit 90) *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P656 y[1] w[67c23]; P{w[+mC]=lacW}k07118, l(2)k07118[k07118]/CyO *F Chromosome(s) affected: 2 *F Insertion site: 029C01-03 *F Date added: 12/19/97 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Istvan Kiss *F Comments: unverified (P{} may or may not cause the lethal), B.D.G.P. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P663 y[1] w[67c23]; P{w[+mC]=lacW}k07219, l(2)k07219[k07219]/CyO *F Chromosome(s) affected: 2 *F Insertion site: 038C05-06 *F Date added: 12/19/97 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Istvan Kiss *F Comments: unverified (P{} may or may not cause the lethal), B.D.G.P. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P698 y[*] w[*]; P{w[+mC]=lacW}B10-2-13 *F Chromosome(s) affected: 2 *F Insertion site: 050C *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: probably has more than 1 insert - at least two sublines have *F developed independently that include flies with intermediate eye *F color, K.M. 11/29/96 *F Embryo-Jan: CNS (stage 14) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P701 y[*] w[*]; P{w[+mC]=lacW}B12-2-6 *F Chromosome(s) affected: 2 *F Insertion site: 053A? *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P707 y[*] w[*]; P{w[+mC]=lacW}C3-2-9 *F Chromosome(s) affected: 2 *F Insertion site: 035C *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 16), trachea (stage 14), gonads (germ line cells, *F stage 15) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P731 y[*] w[*]; P{w[+mC]=lacW}C6-2-29 *F Chromosome(s) affected: 2 *F Insertion site: 049D-50D *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 10), CNS (stage 10), trachea (stage 10), gut *F (stage 10) *F Adult Brain-Vosshall: optic lobe and brain, 2nd and 3rd antennal segments *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P736 y[*] w[*]; P{w[+mC]=lacW}C8-2-17 *F Chromosome(s) affected: 2 *F Insertion site: 052B-D *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS glia (stage 13), trachea (stage 12) *F Adult Brain-Vosshall: optic lobe, central brain, 3rd antennal segment *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P754 P{ry[+t7.2]=A[[10]]O[[31]]}6; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 036C *F Also known as: #AS754 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P758 y[*] w[*]; P{w[+mC]=lacW}E7-2-17 *F Chromosome(s) affected: 2 *F Insertion site: 030B *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 14), sensilla (stage 14) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P769 P{ry[+t7.2]=A[[18]]O[[5]]}8; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 036B-C *F Also known as: #AS769 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P775 y[*] w[*]; P{w[+mC]=lacW}E8-2-17 *F Chromosome(s) affected: 2 *F Insertion site: 052C-D *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS glia (stage 13), sensilla (stage 12) *F Adult Brain-Vosshall: lamina, discrete central brain *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P798 w[1118]; P{w[+t11.7] ry[+t7.2]=wA}1-1 *F Chromosome(s) affected: 2 *F Insertion site: 047A *F Also known as: #GR798, A1-1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P800 w[1118]; P{w[aRsLTR]=w[aRsLTR]}V24-20D *F Chromosome(s) affected: 2 *F Insertion site: 052A *F Also known as: #GR800 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P806 w[1118]; P{w[aRsLTR]=w[aRsLTR]}V3 *F Chromosome(s) affected: 2 *F Insertion site: 047D *F Also known as: #GR806 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P807 z[1] w[1118] cv[1]; P{w[+t*]=white-un2}1.3/CyO *F Chromosome(s) affected: 2 *F Insertion site: 060C07-08 *F Also known as: #GR807 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P811 w[1118]; P{w[+mF] ry[+t7.2]=wF}4-1 *F Chromosome(s) affected: 2 *F Insertion site: 057B *F Also known as: #GR811, F4-1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P815 y[*] w[*]; P{w[+mC]=lacW}E10-2-40 *F Chromosome(s) affected: 2 *F Insertion site: 053D *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), CNS (stage 13), somatic muscle (?) *F Adult Brain-Vosshall: fat body *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P842 y[*] w[*]; P{w[+mC]=lacW}A3-2-66/CyO *F Chromosome(s) affected: 2 *F Insertion site: 039E-040A *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: expression of w+m limited to approximately 1/4 to 1/3 of the *F anterior portion of the eye, Daniel Barbash, 3/1/98 *F Embryo-Jan: epidermis (stage 12), CNS (stage 12), visceral muscle (stage *F 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P847 w[1118]; P{w[+t9.8] ry[+t7.2]=wD}1 *F Chromosome(s) affected: 2 *F Insertion site: 025C *F Also known as: #GR847, D1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P851 w[1118]; P{w[+t11.7] ry[+t7.2]=wA}3-1 *F Chromosome(s) affected: 2 *F Insertion site: 059B *F Also known as: #GR851, A3-1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P883 P{ry[+t*]=Delta0-1}36/CyO; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 038A *F Also known as: #GR883 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Comments: presumably deletion of P ORF0 & ORF1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P884 w[1118]; P{w[aRsLTR]=w[aRsLTR]}V24-35 *F Chromosome(s) affected: 2 *F Insertion site: 060D *F Also known as: #GR884 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P902 y[*] w[*]; P{w[+mC]=lacW}B6-2-25 *F Chromosome(s) affected: 2 *F Insertion site: 051D *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 14), somatic muscle (stage 14), triplet cells *F (unknown structure, stage 15) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P908 w[1118]; P{w[+t11.7] ry[+t7.2]=wA}4-30 *F Chromosome(s) affected: 2 *F Insertion site: 030C *F Also known as: #BL908, A4-30 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bob Levis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P914 w[1118]; P{ry[+t7.2]=wG}2 *F Chromosome(s) affected: 2 *F Insertion site: 037A *F Also known as: #BL914, G2 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bob Levis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P947 CyO, P{ry[+t7.2]=lArB}A103.1M2/b[1] Adh[*] cn[*] l(2)**; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 053C-D *F Also known as: #WG947 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P952 y[1] w[67c23]; P{w[+mC]=lacW}k09918, RpL30[k09918]/CyO *F Chromosome(s) affected: 2 *F Insertion site: 056F08-15 *F Date added: 12/19/97 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Istvan Kiss *F Comments: cytology from H.Bellen, B.D.G.P.; unverified plus -- P shown not to *F cause lethality, K.M. 12/8/98 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P974 CyO, P{ry[+t7.2]=lArB}A217.1M2/b[1] Adh[*] cn[*] l(2)**; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 045D-E *F Also known as: #WG974 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1000 CyO, P{ry[+t7.2]=lArB}A350.1M2/b[1] Adh[*] cn[*] l(2)**; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 059E-F *F Also known as: #WG1000 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: blue balancer, lacZ in Embryo: CNS, anal pads (rim), salivary glands, *F head, amnioserosa & dorsal epidermis boundary; in Adult Ovary: *F follicle cells, border cells, per Hugo Bellen, K.M. 8/10/98 *F Embryo-Bellen: CNS, anal pads (rim), salivary glands, head, amnioserosa & *F dorsal epidermis boundary *F Adult Ovary-Bellen: follicle cells, border cells *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1033 CyO, P{ry[+t7.2]=lArB}A507.2M2, chif[A507]/b[1] Adh[*] cn[*] *F l(2)**; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 035D-E *F Also known as: #WG1033 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: blue balancer, lacZ in Embryo: pole cells, brain, posterior *F spiracles; in Adult Ovary: no staining, per Hugo Bellen, K.M. 8/10/98 *F Embryo-Bellen: pole cells, brain, posterior spiracles *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1102 y[1] w[67c23]; P{w[+mC]=lacW}k13720, l(2)k13720[k13720]/CyO *F Chromosome(s) affected: 2 *F Insertion site: 026C02-03 *F Date added: 3/23/98 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Istvan Kiss *F Comments: unverified (P{} may or may not cause the lethal), B.D.G.P. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1170 P{ry[+t7.2]=lArB}A193.5F3; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 034D *F Also known as: #WG1170 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: site from Ueli Grossniklaus, 4/92 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1228 P{ry[+t7.2]=23.5}T54; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 053D10-11 *F Also known as: #DP1228 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T54+h23.5, site from Bob Hardy, 12/90 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1229 P{ry[+t7.2]=26.4}T154; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 058A *F Also known as: #DP1229 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T154+h26.4 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1233 P{ry[+t7.2]=23.5}T57; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 026A *F Also known as: #DP1233 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T57+h23.5 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1240 P{ry[+t7.2]=23.4}T13B; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 049A *F Also known as: #DP1240 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T13B+h23.4 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1249 P{ry[+t7.2]=26.4}T144; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 025A *F Also known as: #DP1249 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T144+h26.4 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1283 CyO, P{ry[+t7.2]=lArB}A130.1F2/b[1] Adh[*] cn[*] l(2)**; ry[506] *F Chromosome(s) affected: 2 *F Insertion site: 058E *F Also known as: #WG1283 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu # *U FBrf0108526 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.17 *T personal communication to FlyBase *u *F *F *********************************************** *F *F Bloomington stock center records, June 1999. *F First chromosome insertions (subset). *F *F >>> *F B-#P20 y[*] w[*] P{w[+mC]=lacW}3-76a *F Chromosome(s) affected: 1 *F Insertion site: 017-018 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Y. N. Jan *F Comments: good single element ammo *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P78 P{y[+t7.7] ry[+t7.2]=Car20y}SF8, y[*] w[*] *F Chromosome(s) affected: 1 *F Insertion site: 001A-B? *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: E. H. Grell *F Comments: site is 1-0.0, near sc *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P120 P{ry[+t7.2]=ry11}1F; cn[1]; mwh[1] ry[506] e[1] *F Chromosome(s) affected: 1 *F Insertion site: 001F *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: single element ammo (JSK #9) *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P194 y[*] w[*] P{y[+t7.7] ry[+t7.2]=Car20y}SF6 *F Chromosome(s) affected: 1 *F Insertion site: 016-017? *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: E. H. Grell *F Comments: mapped to 1-59, near os *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P202 P{ry[+t7.2]=HB$D-23}9E; Adh[fn6] cn[1]; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 009E *F Also known as: #JL202 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P209 P{ry[+t7.2]=HB$D-89}18F; Adh[fn6] cn[1]; ry[502] *F Chromosome(s) affected: 1 *F Insertion site: 018F04 (?) *F Also known as: #JL209 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Comments: site shown as 19A in ref, KM *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P213 P{ry[+t7.2]=HB$D-59}8E; Adh[fn6] cn[1]; ry[502] *F Chromosome(s) affected: 1 *F Insertion site: 008E *F Also known as: #JL213 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P304 P{ry[+t8.1]=S38Z.74}6; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 005D-E ?? *F Also known as: #AS304 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: G. Mardon says insert at 53F, no insert on X *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P314 P{ry[+t7.2]=R7.7}4; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 006C *F Also known as: #AS314 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P316 P{ry[+t7.2]=R7.7}7; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 017B *F Also known as: #AS316 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P319 P{ry[+t8.1]=ry1}R301.2; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 012D *F Also known as: #AS319 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P334 P{ry[+t8.1]=ry1}R403.1; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 007D *F Also known as: #AS334 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P336 P{ry[+t8.1]=ry1}R404.2; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 009A-D *F Also known as: #AS336 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P338 P{ry[+t8.1]=ry1}R501.1; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 004D *F Also known as: #AS338 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P364 P{ry[+t7.2]=S6.9}2; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 006F *F Also known as: #AS364 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P369 P{ry[+t7.2]=S6.9}10; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 009D *F Also known as: #AS369 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: G. Mardon found insert at 9D (originally shown as 2C or 9C), 3/93 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P370 P{ry[+t7.2]=S6.9}11; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 016B *F Also known as: #AS370 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P394 P{ry[+t7.2]=SRS3.9}2; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 012E01-02 *F Also known as: #AS394 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: M. Axton localized to 12E1-2 (originally shown as 12BC) *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P405 P{ry[+t7.2]=ry11}R701.1; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 009E *F Also known as: #AS405 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P407 P{ry[+t7.2]=ry11}R702.1; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 001F *F Also known as: #AS407 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P410 P{ry[+t7.2]=ry11}R704.2; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 001F *F Also known as: #AS410 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P411 P{ry[+t7.2]=ry11}R704.3; ry[42] *F Chromosome(s) affected: 1 *F Insertion site: 018A *F Also known as: #AS411 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P417 P{ry[+t8.1]=BS2.7A}3; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 018D *F Also known as: #AS417 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P423 P{ry[+t8.1]=BS2.7B}10; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 015D-E *F Also known as: #AS423 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P425 P{ry[+t8.1]=BS2.7B}12; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001CD *F Also known as: #AS425 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P425 P{ry[+t8.1]=BS2.7B}12; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001CD *F Also known as: #AS425 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P438 P{ry[+t8.1]=SB2.1B}6; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 013B09-C01 *F Also known as: #AS438 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: originally shown as SB2.1-2, 83BC; M. Axton localized to 13CD, T. *F Hsieh to 13B9-C1, topoI gene; must be SB2.1-6 - KM *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P441 P{ry[+t8.1]=SB2.1B}5; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 009B *F Also known as: #AS441 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P547 w[1118] sev[14] P{w[+mW.hs]=sev2}ch41 *F Chromosome(s) affected: 1 *F Insertion site: 007D1-2 *F Also known as: T ch41 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #16; first short exon of sev cDNA only; rescues when heat *F shocked *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P549 w[1118] sev[14] P{w[+mW.hs]=sev3}215 *F Chromosome(s) affected: 1 *F Insertion site: 020D *F Also known as: T sev-Met2242.215 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #29; ATP binding site mutant (M3) in genomic sev *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P554 P{ry[+t7.2]=lArB}47; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 003F *F Also known as: ET 47 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #177 *F Larva-Hafen: eye disc, band behind morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P588 P{ry[+t7.2]=lArB}103; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 012B *F Also known as: ET 103 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #182 *F Larva-Hafen: eye disc, clusters behind the morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P589 P{ry[+t7.2]=lArB}112; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 010B1-2 *F Also known as: ET 112 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #184 *F Larva-Hafen: eye disc, behind the morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P590 P{ry[+t7.2]=lArB}154; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 010B1-2 *F Also known as: ET 154 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #185 *F Larva-Hafen: eye disc, behind the morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P592 P{ry[+t7.2]=lArB}175; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001E03 *F Also known as: ET 175 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #187 *F Larva-Hafen: eye disc, band behind the morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P594 P{ry[+t7.2]=lArB}195; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 009E01-03 *F Also known as: ET 195 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #189 *F Larva-Hafen: eye disc, stronger staining behind than ahead of the *F morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P757 P{ry[+t7.2]=A[[17]]O[[15]]}3; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 004E-F *F Also known as: #AS757 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P783 P{ry[+t7.2]=A[[25]]O[[4]]}10; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 012C *F Also known as: #AS783 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P793 P{ry[+t7.2]=hsp0-1}8; st[1] ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 002B *F Also known as: #GR793 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Comments: presumably Hsp70:P-ORF0&1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P799 P{w[E] ry[+t7.2]=wE}1, w[1118] *F Chromosome(s) affected: 1 *F Insertion site: 019F *F Also known as: #GR799, E1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P809 P{w[E] ry[+t7.2]=wE}5 w[1118] *F Chromosome(s) affected: 1 *F Insertion site: 002A *F Also known as: #GR809, E5 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P813 w[1118] P{w[+t9.51] ry[+t7.2]=wH}4 *F Chromosome(s) affected: 1 *F Insertion site: 006A-B *F Also known as: #GR813, H4 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P817 z[1] w[1118] cv[1] P{w[+t*]=white-un2}9.3 *F Chromosome(s) affected: 1 *F Insertion site: 019E *F Also known as: #GR817 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P850 w[1118] P{w[E] ry[+t7.2]=wE}2 *F Chromosome(s) affected: 1 *F Insertion site: 012B *F Also known as: #GR850, E2 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P855 z[1] w[1118] P{w[+t14.3]=wB}1-2 *F Chromosome(s) affected: 1 *F Insertion site: 017D-E *F Also known as: #GR855, B1-2 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P856 w[*] P{w[+t*]=white-un2}20D *F Chromosome(s) affected: 1 *F Insertion site: 010A *F Also known as: #GR856 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P857 P{ry[+t7.2]=hsp0-1}22; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 009D *F Also known as: #GR857 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Comments: presumably Hsp70:P-ORF0&1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P870 w[1118] P{w[+tAR] ry[+t7.2AR]=wA[R]}024/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 020C *F Also known as: #GR870 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P911 w[1118] P{w[E] ry[+t7.2]=wE}sd[E3] *F Chromosome(s) affected: 1 *F Insertion site: 013E-F *F Also known as: #BL911, E3 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bob Levis *F Comments: = sdE3 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P923 P{ry[+t7.2]=wG}4; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 013E-F *F Also known as: #BL923 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bob Levis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P928 w[1118] P{w[+tAR] ry[+t7.2AR]=wA[R]}4-20/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 020D *F Also known as: #BL928, AR4-20 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bob Levis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P930 w[1118] P{w[+tAR] ry[+t7.2AR]=wA[R]}4-12/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 012B-C *F Also known as: #BL930, AR4-12 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bob Levis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1085 P{ry[+t7.2]=lArB}A317.1F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 019C *F Also known as: #WG1085 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1103 P{ry[+t7.2]=lArB}A461.1F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001A *F Also known as: #WG1103 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1131 P{ry[+t7.2]=lArB}A168.1F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001C *F Also known as: #WG1131 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: midgut subset, CNS subset, PNS, head *F Adult Ovary-Bellen: epithelial sheath *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1136 P{ry[+t7.2]=lArB}A374.2F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 012B *F Also known as: #WG1136 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: hindgut, anal pads, head, epidermis *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >> *F B-#P1152 P{ry[+t7.2]=lArB}A171.1F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001A *F Also known as: #WG1152 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1164 P{ry[+t7.2]=lArB}A297.1F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 002D *F Also known as: #WG1164 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: G. Mardon confirmed 002D - 3/93 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1167 P{ry[+t7.2]=lArB}A522.2F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 006D-E *F Also known as: #WG1167 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1168 P{ry[+t7.2]=lArB}A135.2F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 019A *F Also known as: #WG1168 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1219 P{ry[+t7.2]=23.3}T12; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001E-F *F Also known as: #DP1219 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T12+h23.3/1EF *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >> *F B-#P1220 P{ry[+t7.2]=26.2}T21; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 012F-013A *F Also known as: #DP1220 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T21+h26.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1223 P{ry[+t7.2]=23.1}T134; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 020B-C *F Also known as: #DP1223 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T134+h23.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1226 P{ry[+t7.2]=23.3}T72; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 017B *F Also known as: #DP1226 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T72+h23.3 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1238 P{ry[+t7.2]=26.1}T59; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 001B-C *F Also known as: #DP1238 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T59+h26.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1260 P{ry[+t7.2]=lArB}A418.1F1; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 003C01-07 *F Also known as: #WG1260 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: muscle, PNS, head *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1288 P{ry[+t7.2]=lArB}A106.1F1/FM6; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 007C *F Also known as: #WG1288 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1297 P{ry[+t7.2]=P-Sal}I16/C(1)DX, y[1] f[1]; bw[1]; st[1] *F Chromosome(s) affected: 1 *F Insertion site: 014C *F Also known as: #BE1297 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: XSalI-I16, produces P repressor, see Genetics 123:815 (1989) for *F repressor properties *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1298 P{ry[+t7.2]=P-Sal}I35/C(1)DX, y[1] f[1]; bw[1]; st[1] *F Chromosome(s) affected: 1 *F Insertion site: 003D *F Also known as: #BE1298 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: XSalI-I35, produces P repressor *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1299 P{ry[+t7.2]=P-Sal}I70; bw[1]; st[1] *F Chromosome(s) affected: 1 *F Insertion site: 016C *F Also known as: #BE1299 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: XSalI-I70, produces P repressor, B.E.; sent as C(1)DX, y f, but *F females no longer y f, assume stock has gone homozygous for P{P- *F Sal}I70, K.M. 2/8/95 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1302 w[*] P{w[+mC]=CaSpeR}Sh32.1a P{CaSpeR}SH32.1b/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 014B, 017C *F Also known as: #BE1302 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: Sh32.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1303 w[*] P{w[+mC]=CaSpeR}C16.1a P{CaSpeR}C16.1b/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 017C, 019C *F Also known as: #BE1303 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: C16.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1304 w[*] P{w[+mC]=CaSpeR}C19.1a P{CaSpeR}C19.1b/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 006A, 017C *F Also known as: #BE1304 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: C19.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1305 w[*] P{w[+mC]=CaSpeR}C34.3/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 011D *F Also known as: #BE1305 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: C34.3 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1306 w[*] P{w[+mC]=CaSpeR}C75.2/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 018F *F Also known as: #BE1306 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: C75.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1307 w[*] P{w[+mC]=CaSpeR}C100.2a P{CaSpeR}C100.2b/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 002B, 017C *F Also known as: #BE1307 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: C100.2; G. Mardon confirmed 002B site - 3/93 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1313 P{w[+mC]=CaSpeR}cx20.8/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 007D *F Also known as: #BE1313 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx20.8 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1314 P{w[+mC]=CaSpeR}cx20A.4/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 001B *F Also known as: #BE1314 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx20A.4 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1315 P{w[+mC]=CaSpeR}cx27.1/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 018F *F Also known as: #BE1315 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx27.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1316 P{w[+mC]=CaSpeR}cx30.2/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 001B *F Also known as: #BE1316 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx30.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1317 P{w[+mC]=CaSpeR}cx30A.1/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 005B *F Also known as: #BE1317 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx30A.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1318 P{w[+mC]=CaSpeR}cx31A.2a P{CaSpeR}cx31A.2b/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 001C, 016C *F Also known as: #BE1318 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx31A.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1319 P{w[+mC]=CaSpeR}cx31A.3/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 010E *F Also known as: #BE1319 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx31A.3; Rob Jackson says this stock carries a long-period circadian *F rhythm mutation that maps close to or to the left of w, probably a *F perL* allele, but complementation with per not done, K.M. 3/24/96 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1320 P{w[+mC]=CaSpeR}cx32.4/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 012E *F Also known as: #BE1320 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx32.4, pale yellow ovoid eyes *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1321 P{w[+mC]=CaSpeR}cx34.6/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 006D *F Also known as: #BE1321 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx34.6 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1322 P{w[+mC]=CaSpeR}cx34.11a P{CaSpeR}cx34.11b/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 003D, 006C-E *F Also known as: #BE1322 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx34.11 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1323 P{w[+mC]=CaSpeR}cx35.1/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 006E *F Also known as: #BE1323 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx35.1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1324 P{w[+mC]=CaSpeR}cx120.10/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 017C *F Also known as: #BE1324 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx120.10 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1325 P{w[+mC]=CaSpeR}cx120A.10/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 002A *F Also known as: #BE1325 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx120A.10 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1326 P{w[+mC]=CaSpeR}cx131.6/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 010E *F Also known as: #BE1326 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx131.6 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1327 P{w[+mC]=CaSpeR}cx134.13/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 006D *F Also known as: #BE1327 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx134.13 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1328 P{w[+mC]=CaSpeR}cx134.16/C(1)DX, y[1] w[1] f[1] *F Chromosome(s) affected: 1 *F Insertion site: 003D-E *F Also known as: #BE1328 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Bill Engels *F Comments: cx134.16 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1468 P{ry[+t7.2]=lArB}lz[A27.1F1]; ry[506] *F Chromosome(s) affected: 1 *F Insertion site: 008C-D *F Also known as: #WG1468 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1837 P{w[+mC]=lacW}G0147a w[67c23] P{lacW}G0147b, l(1)G0147[G0147]/FM7c *F Chromosome(s) affected: 1 *F Insertion site: 003A03, 018C01-02 *F Date added: 2/25/99 *F Donor: Ulrich Schaefer & Herbert Jackle *F Comments: in situ signal weak at 3A3, wgSp-1 may be segregating, chromosome *F is lethal, but which, if either, P{} is responsible is not known, *F U.S. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1842 P{w[+mC]=lacW}G0268a P{lacW}G0268b w[67c23], l(1)G0268[G0268]/FM7c *F Chromosome(s) affected: 1 *F Insertion site: 002B16-18, 003A03 *F Date added: 4/16/99 *F Donor: Ulrich Schaefer & Herbert Jackle *F Comments: chromosome is lethal, but which, if either, P{} is responsible is not *F known, U.S. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1847 P{w[+mC]=lacW}G0244a w[67c23] P{lacW}G0244b, l(1)G0244[G0244]/FM7c *F Chromosome(s) affected: 1 *F Insertion site: 003A01-04, 004D01-02 *F Date added: 4/16/99 *F Donor: Ulrich Schaefer & Herbert Jackle *F Comments: chromosome is lethal, but which, if either, P{} is responsible is not *F known, U.S. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F # *U FBrf0108527 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.19 *T personal communication to FlyBase *u *F *F Bloomington stock center records, June 1999. *F Third chromosome insertions (subset), including P{hsneo} lines. *F *F >>> *F B-#P1 P{hsneo}102 mwh[1] red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 061A-B *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P2 mwh[1] P{hsneo}103 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 063C *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P3 mwh[1] P{hsneo}104 red[]1 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 065E *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P4 mwh[1] P{hsneo}105 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 066B *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P5 mwh[1] P{hsneo}106 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 066D *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P6 mwh[1] P{hsneo}107 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 066E *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P7 mwh[1] P{hsneo}108 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 067B *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P8 mwh[1] P{hsneo}109 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 067C *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P9 P{ry[+t7.2]=PZ}rC56 *F Chromosome(s) affected: 3 *F Insertion site: 094B-C *F Date added: 10/24/95 *F Donor: Christian Klambt *F Comments: pattern identical to 3-109, C.K. *F Embryo-Klambt: glial cells *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Klambt == Christian Klambt *F *F >>> *F B-#P10 w[1118]; P{w[+mW.hs]=lwB}AA142 e[1]/TM3, Sb[1] *F Chromosome(s) affected: 3 *F Insertion site: 066D *F Date added: 10/24/95 *F Donor: Christian Klambt *F Comments: excellent marker for midline glial cells, C.K.; probably e1, might *F be e4, K.M.; homozygous viable, but does better over balancer, K.M. *F 7/2/98 *F Embryo-Klambt: midline glial cells *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Klambt == Christian Klambt *F *F >>> *F B-#P11 mwh[1] P{hsneo}112 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 068F *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P12 mwh[1] P{hsneo}113 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 068F *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P13 mwh[1] P{hsneo}114 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 070C *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P14 mwh[1] P{hsneo}115 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 071A *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P15 mwh[1] P{hsneo}116 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 071E *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P16 mwh[1] P{hsneo}117 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 075B *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P18 mwh[1] P{hsneo}119 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 075D *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P19 mwh[1] P{hsneo}120 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 075F *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P21 mwh[1] P{hsneo}122 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 077C *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P22 mwh[1] P{hsneo}123 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 078A *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P23 mwh[1] P{hsneo}124 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 079B-C *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P24 mwh[1] P{hsneo}125 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 082D *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P25 mwh[1] P{hsneo}126 red[1] e[1], l(3)*[]*/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 083B01-02 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P26 mwh[1] P{hsneo}127 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 085A *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P27 mwh[1] P{hsneo}128 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 086A *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P28 mwh[1] P{hsneo}129 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 086C *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P29 mwh[1] P{hsneo}130 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 087A *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P31 mwh[1] P{hsneo}132 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 087E *F Also known as: #AS31, neo132 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P32 mwh[1] P{hsneo}133 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 087F-88A *F Also known as: #AS32, neo133 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P33 mwh[1] P{hsneo}134 red[1] e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 088A *F Also known as: #AS33, neo134 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.ed *F *F >>> *F B-#P34 mwh[1] red[1] P{hsneo}135 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 088C *F Also known as: #AS34, neo135 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P35 mwh[1] red[1] P{hsneo}136 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 088E *F Also known as: #AS35, neo136 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P36 mwh[1] red[1] P{hsneo}137 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 088F *F Also known as: #AS36, neo137 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P37 mwh[1] red[1] P{hsneo}138 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 089B *F Also known as: #AS37, neo138 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P38 mwh[1] red[1] P{hsneo}139 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 089E *F Also known as: #AS38, neo139 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P39 mwh[1] red[1] P{hsneo}140 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 090D *F Also known as: #AS39, neo140 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P40 mwh[1] red[1] P{hsneo}141 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 091D *F Also known as: #AS40, neo141 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P41 mwh[1] red[1] P{hsneo}142 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 091E *F Also known as: #AS41, neo142 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P43 mwh[1] red[1] P{hsneo}144 e[1], l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 092A-B *F Also known as: #AS43, neo144 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P45 mwh[1] red[1] e[1] P{hsneo}146, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 095C *F Also known as: #AS45, neo146 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P46 mwh[1] red[1] e[1] P{hsneo}147, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 095F *F Also known as: #AS46, neo147 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P47 mwh[1] red[1] e[1] P{hsneo}148, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 096D *F Also known as: #AS47, neo148 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P48 mwh[1] red[1] e[1] P{hsneo}149, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 096F *F Also known as: #AS48, neo149 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P49 mwh[1] red[1] e[1] P{hsneo}150, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 097F *F Also known as: #AS49, neo150 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F *F >>> *F B-#P50 mwh[1] red[1] e[1] P{hsneo}151, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 099F *F Also known as: #AS50, neo151 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P51 mwh[1] red[1] e[1] P{hsneo}152, l(3)*[*]/TM3, Sb[1] Ser[1] *F Chromosome(s) affected: 3 *F Insertion site: 100A *F Also known as: #AS51, neo152 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F *F >>> *F B-#P192 y[1] ac[1]; P{y[+t7.7] ry[+t7.2]=Car20y}96E, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 096E *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Victor Corces *F Comments: y and ac alleles not specified *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P195 y[1] ac[1]; ry[506] P{y[+t7.7] ry[+t7.2]=Car20y}66B? *F Chromosome(s) affected: 3 *F Insertion site: 066B? *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Victor Corces *F Comments: mapping from E. H. Grell, site is 3-25.4 (1.1 mu to the left of h); *F this insert is unstable, K.M. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P201 Adh[fn6] cn[1]; P{ry[+t7.2]=HBDelta-194}84E ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 084E *F Also known as: #JL201 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P203 Adh[fn6] cn[1]; P{ry[+t7.2]=HBDelta-23}88A, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 088A *F Also known as: #JL203 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P224 Adh[fn6] cn[1]; P{ry[+t7.2]=Hsp70B-51}71AB ry[502] *F Chromosome(s) affected: 3 *F Insertion site: 071A-B *F Also known as: #JL224 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P225 Adh[fn6] cn[1]; P{ry[+t7.2]=hsp26-lacZ}84D ry[502] *F Chromosome(s) affected: 3 *F Insertion site: 084D *F Also known as: #JL225 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: John Lis *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P229 P{ry[+t7.2]=RP49}B1 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 080A *F Also known as: #JM229 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Comments: B1 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P232 P{ry[+t7.2]=RP49}F2 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 080A *F Also known as: #JM232 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Comments: F2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P325 P{ry[+t8.1]=ry1}R307.1 ry[42] *F Chromosome(s) affected: 3 *F Insertion site: 087A *F Also known as: #AS325 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P329 P{ry[+t8.1]=ry1}R311.1 ry[42] *F Chromosome(s) affected: 3 *F Insertion site: 086D *F Also known as: #AS329 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: could be P{ry3} *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >> *F B-#P383 ry[506] P{ry[+t8.1]=S38Z.247}3 *F Chromosome(s) affected: 3 *F Insertion site: 093A *F Also known as: #AS383 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P395 ry[506] P{ry[+t7.2]=SRS3.9}3 *F Chromosome(s) affected: 3 *F Insertion site: 099D *F Also known as: #AS395 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P396 P{ry[+t7.2]=SRS3.9}4 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 061E *F Also known as: #AS396 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P401 ry[42] P{ry[+t7.2]=ry10}R601.1 *F Chromosome(s) affected: 3 *F Insertion site: 096A-B *F Also known as: #AS401 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Comments: ref shows site as 95A, DC changed to 96A-B, no further info. *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P402 ry[42] P{ry[+t7.2]=ry10}R602.1 *F Chromosome(s) affected: 3 *F Insertion site: 098C *F Also known as: #AS402 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P412 ry[42] P{ry[+t7.2]=ry11}R705.1 *F Chromosome(s) affected: 3 *F Insertion site: 098C *F Also known as: #AS412 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P415 ry[506] P{ry[+t8.1]=BS2.7A}1 *F Chromosome(s) affected: 3 *F Insertion site: 098A *F Also known as: #AS415 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P427 P{ry[+t7.2]=S38M}1 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 071C *F Also known as: #AS427 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P429 ry[506] P{ry[+t7.2]=S38M}3 *F Chromosome(s) affected: 3 *F Insertion site: 096B *F Also known as: #AS429 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P432 ry[506] P{ry[+t7.2]=S38M}7 *F Chromosome(s) affected: 3 *F Insertion site: 097F *F Also known as: #AS432 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P436 P{ry[+t8.1]=S38Z.2}6 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 063C *F Also known as: #AS436 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Allan Spradling *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P548 w[1118] sev[14]; P{w[+mW.hs]=sev2}ch50 *F Chromosome(s) affected: 3 *F Insertion site: 093B *F Also known as: T ch50 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #18; first short exon of sev cDNA only; rescues when heat *F shocked *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P551 P{ry[+t7.2]=lArB}17, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 092F01-03 *F Also known as: ET 17 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #140 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P552 P{ry[+t7.2]=lArB}36, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 092D *F Also known as: ET 36 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #141 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P587 P{ry[+t7.2]=lArB}99, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 066B1-2 *F Also known as: ET 99 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #181 *F Larva-Hafen: eye disc, band behind morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P591 P{ry[+t7.2]=lArB}166, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 089F *F Also known as: ET 166 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #186 *F Larva-Hafen: eye disc, band behind the morphogenetic furrow *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Hafen == Ernst Hafen *F *F >>> *F B-#P753 y[*] w[*]; P{w[+mC]=lacW}E6-3-7/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 086E *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: CNS (stage 12), sensilla (stage 13), gut, garland cells *F Adult Brain-Vosshall: medulla, central brain *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P763 y[*] w[*]; P{w[+mC]=lacW}E7-3-49 *F Chromosome(s) affected: 3 *F Insertion site: 089C-D *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: epidermis (stage 11), CNS (stage 13), amnioserosa, triplet *F cells (unknown structure) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P764 y[*] w[*]; P{w[+mC]=lacW}E7-3-52 *F Chromosome(s) affected: 3 *F Insertion site: 079E *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 12), CNS (stage 13), triplet cells (unknown *F structure), imaginal discs *F Adult Brain-Vosshall: optic lobe *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P796 w[1118]; P{w[+mF] ry[+t7.2]=wF}2 *F Chromosome(s) affected: 3 *F Insertion site: 086C *F Also known as: #GR796, F2 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P821 z[1] w[11E4] f[5]; P{w[+t14.3]=wB}1-1 *F Chromosome(s) affected: 3 *F Insertion site: 089A *F Also known as: #GR821, B1-1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P833 w[1118]; P{w[+t11.7] ry[+t7.2]=wA}4-4 *F Chromosome(s) affected: 3 *F Insertion site: 100F *F Also known as: #GR833, A4-4 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P834 w[1118]; P{w[+mF] ry[+t7.2]=wF}4-3 *F Chromosome(s) affected: 3 *F Insertion site: 097B *F Also known as: #GR834, F4-3 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P862 z[1] w[11E4] f[5]; P{w[+t*]=white-un2}31/TM6 *F Chromosome(s) affected: 3 *F Insertion site: 063D *F Also known as: #GR862 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P872 w[1118]; P{w[+t9.51] ry[+t7.2]=wH}1 *F Chromosome(s) affected: 3 *F Insertion site: 078C-D *F Also known as: #GR872, H1 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P877 z[1] w[11E4] f[5]; P{w[+t*]=white-un2}22 *F Chromosome(s) affected: 3 *F Insertion site: 089B *F Also known as: #GR877 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P881 z[1] w[11E4] f[5]; P{w[+t*]=white-un2}30 *F Chromosome(s) affected: 3 *F Insertion site: 066C *F Also known as: #GR881 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Gerry Rubin *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P933 y[*] w[*]; ?/CyO; P{w[+mC]=lacW}B8-3-38 *F Chromosome(s) affected: 3 *F Insertion site: 067C *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: sent as B8-3-38, viable insert at 67C, but Cy balancer present (CyO *F is a guess), KM 1/95; checked location of insert by segregation and *F it is on 3, K.M. 4/10/96; eye-color: dark orange *F Embryo-Jan: CNS (stage 11), sensilla (stage 13), trachea (stage 13), *F visceral muscle (stage 13), external sensory organ support *F cells *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P950 P{ry[+t7.2]=lArB}A119.1M3 ry[506]/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 085B *F Also known as: #WG950 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present *F Embryo-Bellen: midgut, head, epidermis *F Adult Ovary-Bellen: follicle cells (stage 8) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P987 P{ry[+t7.2]=lArB}A265.2M3 ry[506]/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 069C *F Also known as: #WG987 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P993 ry[506] P{ry[+t7.2]=lArB}A321.3M3/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 081F *F Also known as: #WG993 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1002 ry[506] P{ry[+t7.2]=lArB}A353.2M3/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 096F05-08 *F Also known as: #WG1002 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present; site from Ueli *F Grossniklaus, 4/92 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1027 P{ry[+t7.2]=lArB}A490.2M3 ry[506]/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 068F *F Also known as: #WG1027 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: site from Ueli Grossniklaus, 4/92; whether P{} is responsible for *F lethality is unknown, but all adults are balanced, K.M. 3/5/96 *F Embryo-Bellen: midgut, head *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1052 P{ry[+t7.2]=lArB}A77.1M3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 070A *F Also known as: #WG1052 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: weak stock *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1064 P{ry[+t7.2]=lArB}B21.1M3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 070F *F Also known as: #WG1064 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: muscle *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1075 P{ry[+t7.2]=lArB}B72.1M3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 064D *F Also known as: #WG1075 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: site from Ueli Grossniklaus, 4/92 *F Embryo-Bellen: midgut subset, proventriculus, hindgut, posterior *F spiracles, anal pads *F Adult Ovary-Bellen: follicle cells (stages 9-14), squamous follicle cells *F (stages 12-14) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1088 P{ry[+t7.2]=lArB}A143.1F3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 086B *F Also known as: #WG1088 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1091 ry[506] P{ry[+t7.2]=lArB}A292.2F3 *F Chromosome(s) affected: 3 *F Insertion site: 088A-B *F Also known as: #WG1091 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: midgut subset, proventriculus, gastric caecae *F Adult Ovary-Bellen: germarium (regions 1-3), follicle cells (stages 1- *F 14), squamous follicle cells (stages 9-14) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1092 P{ry[+t7.2]=lArB}A140.1F3 ry[506]/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 085E *F Also known as: #WG1092 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1120 ry[506] P{ry[+t7.2]=lArB}A189.2F3 *F Chromosome(s) affected: 3 *F Insertion site: 095B *F Also known as: #WG1120 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: site from Ueli Grossniklaus, 4/92 *F Embryo-Bellen: salivary glands, pharynx, malphighian tubules, CNS subset, *F posterior spiracles, head, posterior midgut, foregut *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1151 ry[506] P{ry[+t7.2]=lArB}A256.2F3/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 091B *F Also known as: #WG1151 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1153 P{ry[+t7.2]=lArB}A179.4F3 ry[506]/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 071A-B *F Also known as: #WG1153 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: semilethal? not shown as lethal, but balancer present, site from Ueli *F Grossniklaus, 4/92 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1155 ry[506] P{ry[+t7.2]=lArB}A102.2F3 *F Chromosome(s) affected: 3 *F Insertion site: 100F *F Also known as: #WG1155 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1169 P{ry[+t7.2]=lArB}A131.1F3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 065C-D *F Also known as: #WG1169 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: site from Ueli Grossniklaus, 4/92 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1197 ry[506] P{ry[+t7.2]=lArB}A484.1M3 *F Chromosome(s) affected: 3 *F Insertion site: 094D *F Also known as: #WG1197 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1206 P{ry[+t7.2]=lArB}A55.1M3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 061A *F Also known as: #WG1206 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1221 P{ry[+t7.2]=26.2}T9 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 078E-F *F Also known as: #DP1221 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T9+h26.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1235 P{ry[+t7.2]=23.2}T125 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 061A *F Also known as: #DP1235 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T125+h23.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1242 ry[506] P{ry[+t7.2]=23.2}T99 *F Chromosome(s) affected: 3 *F Insertion site: 098E *F Also known as: #DP1242 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T99+h23.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1250 P{ry[+t7.2]=26.2}T1 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 077E *F Also known as: #DP1250 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T1+h26.2 *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1252 P{ry[+t7.2]=27.1}T109, ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 092C-D *F Also known as: #DP1252 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Daniel Pauli *F Comments: T109+h27.1, this line not described in ref, construct identity is an *F assumption, KM *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P1269 ry[506] P{ry[+t7.2]=lArB}C40.1S3 *F Chromosome(s) affected: 3 *F Insertion site: 100D *F Also known as: #WG1269 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: site from Ueli Grossniklaus, 4/92 *F Embryo-Bellen: unidentified cells *F Adult Ovary-Bellen: follicle cells, border cells *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P2032 P{ry[+t7.2]=lArB}A100.1M3 ry[506] *F Chromosome(s) affected: 3 *F Insertion site: 062A09-B2 *F Also known as: #WG946 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F # *U FBrf0108528 *a Stowers *b S. *t 1999.6.18 *T personal communication to FlyBase *u *F ===================================================================== *F From kcook@bio.indiana.edu Fri Jun 18 11:38:12 1999 *F X-Sender: kcook@sunflower.bio.indiana.edu (Unverified) *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Fri, 18 Jun 1999 10:35:46 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Stowers P{GMR-hid} and P{ey-GAL4.H} insertions *F Mime-Version: 1.0 *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Subject: P{GMR-hid} and P{ey-GAL4.H} insertions *F *F Steve Stowers, Department of Molecular and Cellular Physiology, Stanford *F University School of Medicine, communicated the following information *F concerning insertions of P{GMR-hid} and P{ey-GAL4.H} in stocks he donated *F to the Stock Center. The isolation of the insertions will be described in *F a Genetics paper in press. *F *F 1. P{w[+mC]=GMR-hid} *F *F Insertions on the X, 2R, 3L and 3R were obtained by mobilizing *F P{w[+mC]=GMR-hid]G1. The insertions were not mapped more precisely. *F *F 2. P{w[+*]=ey-GAL4.H} *F *F A third chromosome insertion of P{ey-GAL4.H} was obtained by mobilizing the *F second chromosome insertion described in Hazelett et al. 1998, Development *F 125: 3741-3751. The third chromosome insertion was not mapped more *F precisely. No insertion identifier was assigned to the second chromosome *F insertion of Hazelett et al. Stowers indicated that the visible marker on *F P{ey-GAL4.H} is w[+]. *F *F *F Stowers combined a P{FRT} insertion and a P{GMR-hid} insertion on each of *F the major chromosome arms. Each P{FRT} P{GMR-hid} chromosome was *F mutagenized to induce a linked modifier of the P{GMR-hid} phenotype. These *F modifier mutations are probably cell lethals. *F *F From the communication from Stowers (6/17/99): *F The cell lethal mutation on chromosome arm 2L was induced by EMS in a *F screen I did. Adding the recessive cell lethal to the GMR-hid chromosome *F arm significantly improves morphology of the resulting adult eye. The CL *F mutations on the other chromosome arms are only putative cell lethal *F mutations because I screened for these on the basis of the improved eye *F morphology phenotype by directly mutagenizing the GMR-hid chromosomes with *F gamma rays. I didn't generate recessive cell lethals and then recombine *F them onto the GMR-hid chromosome arms. I know nothing about the allelism *F of the putative cell lethals. *F *F *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 *F *F *F ----- End Included Message ----- *F *F # *U FBrf0108529 *a Schaefer *b U. *c H. *d Jackle *e Y. *f He *g H. *h Bellen *i T. *j Laverty *k G. *l Rubin *t 1999.6.15 *T personal communication to FlyBase *u *F Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Subject: Goettingen lethals - set 2 *F Dated: 15 June 1999 *F *F Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of *F Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map the *F P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read by *F both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines is *F caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView. *F This communication includes revised insertion sites for some of the lines included in FBrf0108119, followed by new *F alleles and transposon insertions. *F *F Alleles with revised insertion sites (alleles presumed to be caused by P{lacW} insertions): *F Original Revised *F l(1)G0028[G0028] 11B3-6 12E1-4 *F l(1)G0030[G0030] 5D3-6 6A *F l(1)G0035[G0035] 6F1-2 6E4-5 *F l(1)G0037[G0037] 1B7-8 1C *F l(1)G0040[G0040] 8B1-4 10C1-2 *F l(1)G0043[G0043] 6F1-2 6F *F l(1)G0054[G0054] 3F4-5 3F1-5 *F l(1)G0056[G0056] 9E3-4 9E1-2 *F l(1)G0057[G0057] 13F1-2 13E8-15 *F l(1)G0061[G0061] 9E3-4 5E1-2 *F l(1)G0063[G0063] 5E1-2 5E1-7 *F l(1)G0066[G0066] 2C1-2 2C *F l(1)G0067[G0067] 8E3-4 8B1-4 *F l(1)G0071[G0071] 7E5-6 7E3-11 *F l(1)G0072[G0072] 9C3-4 9F1-4 *F l(1)G0074[G0074] 16D1-2 16C1-2 *F l(1)G0075[G0075] 9D 10A4-9 *F Actn[G0077] 2C1-2 2C *F l(1)G0078[G0078] 11D1-2 11E (multi-insert line, no change in other insertion site) *F l(1)G0079[G0079] 5C3-6 5C3-9 *F l(1)G0081[G0081] 4B1-2 4B *F l(1)G0083[G0083] 9F1-6 9F *F l(1)G0104[G0104] 12D 12C *F l(1)G0105[G0105] 1E3-4 1F1-2 *F l(1)G0115[G0115] 1C2-3 1C1-3 *F l(1)G0117[G0117] 5C1-2, 5D1-2 5C3-9 (i.e., no longer judged to be a multi-insert line) *F l(1)G0118[G0118] 6E1-2 6E1-5 *F l(1)G0127[G0127] 9E1-2 9E *F l(1)G0130[G0130] 2B1-2 2B1-4 *F *F Revised transposon insertion conclusions (from multi-insert lines): *F *F Original Revision *F P{lacW}G0078a 11D1-2 11E *F P{lacW}G0117a 5C1-2 eliminate *F P{lacW}G0117b 5D1-2 eliminate *F *F New alleles (presumed to be caused by insertion of P{lacW}: *F *F Allele Insertion site *F l(1)G0003[G0003] 17C *F l(1)G0004[G0004] 19C *F l(1)G0012[G0012] 2A1-2 *F l(1)G0013[G0013] 18D1-2 *F l(1)G0014[G0014] 2C1-2 *F l(1)G0020[G0020] 8B1-4 *F l(1)G0022[G0022] semi-lethal 13F1-2 *F l(1)G0024[G0024] 3B *F l(1)G0025[G0025] 5D1-2 *F l(1)G0027[G0027] 13E7-15 *F l(1)G0036[G0036] semi-lethal 6F1-2 *F l(1)G0041[G0041] 18D1-4 *F l(1)G0044[G0044] 2B1-4 *F l(1)G0046[G0046] ? -- multi-insert line: 3A1-4, 12F1-5 *F l(1)G0060[G0060] 11B1-4 *F l(1)G0062[G0062] 19A1-2 *F l(1)G0065[G0065] 10B1-2 *F l(1)G0070[G0070] 17C1-2 *F l(1)G0076[G0076] 9E7-8 *F l(1)G0086[G0086] 19E *F l(1)G0090[G0090] 7A1-5 *F l(1)G0092[G0092] ? -- multi-insert line: 17C1-4, 17D1-4 *F l(1)G0094[G0094] semi-lethal ? -- multi-insert line: 4D1-2, 9E3-4 *F l(1)G0097[G0097] semi-lethal ? -- multi-insert line: 4E, 9C1-2, 12F1-3 *F l(1)G0099[G0099] 7F1-4 *F l(1)G0102[G0102] 10E1-4 *F l(1)G0103[G0103] 12E1-4 *F l(1)G0111[G0111] 10E *F l(1)G0112[G0112] semi-lethal 8F4-7 *F l(1)G0116[G0116] 14F1-3 *F l(1)G0120[G0120] 18F1-2 *F l(1)G0121[G0121] semi-lethal 12D1-2 *F l(1)G0123[G0123] semi-lethal 13E1-6 *F l(1)G0124[G0124] 11B-C *F l(1)G0125[G0125] 19E *F l(1)G0126[G0126] semi-lethal 9F1-2 *F l(1)G0128[G0128] semi-lethal 14B1-4 *F l(1)G0137[G0137] ? -- multi-insert line: 8F1-2, 9A1-3 *F l(1)G0143[G0143] 16B1-3 *F l(1)G0144[G0144] 2F *F l(1)G0147[G0147] ? -- multi-insert line: 3A3, 18C1-2 *F l(1)G0150[G0150] 18F *F l(1)G0153[G0153] 4C13-16 *F l(1)G0154[G0154] 7D14-21 *F l(1)G0156[G0156] 18D1-4 *F l(1)G0161[G0161] ? -- multi-insert line: 7E2-3, 7E5-8 *F l(1)G0164[G0164] 13E *F l(1)G0165[G0165] 17B1-C4 *F l(1)G0168[G0168] 13D1-2 *F l(1)G0169[G0169] 10B1-2 *F l(1)G0174[G0174] 3E *F l(1)G0177[G0177] 5C5-6 *F l(1)G0179[G0179] semi-lethal 19F *F l(1)G0186[G0186] 12D-E2 *F l(1)G0191[G0191] 12C *F l(1)G0193[G0193] 7D1-3 *F l(1)G0196[G0196] 9E1-4 *F l(1)G0198[G0198] 16C1-2 *F l(1)G0199[G0199] ? -- multi-insert line: 7D10-14, 18D10-E *F l(1)G0200[G0200] 8E1-4 *F l(1)G0201[G0201] 13C *F l(1)G0202[G0202] semi-lethal ? -- multi-insert line: 10C1-2, 12B *F l(1)G0203[G0203] semi-lethal 7E5-6 *F l(1)G0205[G0205] semi-lethal 17C-D *F l(1)G0207[G0207] 4C11-14 *F l(1)G0209[G0209] 18E-F *F l(1)G0211[G0211] 3A1-4 *F l(1)G0212[G0212] 14B5-8 *F l(1)G0216[G0216] 7C *F l(1)G0219[G0219] 7E4-8 *F l(1)G0220[G0220] semi-lethal 2B13-C2 *F l(1)G0221[G0221] 14C *F l(1)G0223[G0223] 18F *F l(1)G0228[G0228] 7E3-6 *F l(1)G0229[G0229] semi-lethal 13C1-2 *F l(1)G0233[G0233] 7D3-5 *F l(1)G0237[G0237] 10C1-2 *F l(1)G0239[G0239] 13E13-F2 *F l(1)G0241[G0241] 10D1-3 *F l(1)G0244[G0244] ? -- multi-insert line: 3A1-4, 4D1-2 *F l(1)G0245[G0245] 5C5-8 *F l(1)G0249[G0249] 8E1-4 *F l(1)G0250[G0250] semi-lethal 8E1-8 *F l(1)G0251[G0251] semi-lethal 3A3-4 *F l(1)G0252[G0252] semi-lethal 11C *F l(1)G0254[G0254] 6C *F l(1)G0259[G0259] 1B1-2 *F l(1)G0260[G0260] semi-lethal 14B3-8 *F l(1)G0262[G0262] 13F1-2 *F l(1)G0263[G0263] 3B1-2 *F l(1)G0266[G0266] 18D5-8 *F l(1)G0267[G0267] 10B3-10 *F l(1)G0268[G0268] ? -- multi-insert line: 2B13-18, 3A1-3 *F l(1)G0270[G0270] 9E4-10 *F l(1)G0271[G0271] 3A3-4 *F l(1)G0272[G0272] 14C3-8 *F l(1)G0276[G0276] 10B14-17 *F l(1)G0277[G0277] 12A1-2 *F l(1)G0278[G0278] 13B5-9 *F l(1)G0282[G0282] semi-lethal 18E-F *F l(1)G0283[G0283] 18D5-8 *F l(1)G0284[G0284] 2B1-8 *F l(1)G0286[G0286] 8F *F l(1)G0287[G0287] 5E-F *F l(1)G0290[G0290] 6C4-8 *F l(1)G0293[G0293] 4B1-4 *F l(1)G0296[G0296] 1E *F l(1)G0298[G0298] 11C *F l(1)G0301[G0301] semi-lethal 2B *F l(1)G0307[G0307] ? -- multi-insert line: 7C7-9, 12F1-4 *F l(1)G0309[G0309] 13F1-2 *F l(1)G0310[G0310] 2D *F l(1)G0311[G0311] ? -- multi-insert line: 3A1-4, 18C1-4 *F l(1)G0312[G0312] 5A *F l(1)G0315[G0315] ? -- multi-insert line: 3A1-4, 13F1-4 *F l(1)G0317[G0317] 6F1-4 *F l(1)G0318[G0318] 2B1-8 *F l(1)G0323[G0323] 8B1-4 *F l(1)G0324[G0324] semi-lethal ? -- multi-insert line: 3A1-4, 9E3-4 *F l(1)G0329[G0329] 1B7-10 *F l(1)G0330[G0330] 5C5-8 *F l(1)G0332[G0332] 7E *F l(1)G0345[G0345] 5F1-3 *F l(1)G0346[G0346] semi-lethal 8E1-4 *F l(1)G0355[G0355] semi-lethal 2C1-2 *F l(1)G0360[G0360] 2C7-D4 *F l(1)G0363[G0363] semi-lethal ? -- multi-insert line: 18B1-4, 19E1-2 *F l(1)G0384[G0384] 5A5-9 *F l(1)G0394[G0394] semi-lethal 12C4-5 *F l(1)G0395[G0395] semi-lethal 13E1-4 *F l(1)G0398[G0398] semi-lethal 12C *F l(1)G0409[G0409] 18D5-8 *F l(1)G0423[G0423] semi-lethal ? -- multi-insert line: 2C, 9E1-4 *F l(1)G0427[G0427] 18F *F l(1)G0428[G0428] 19E *F *F New transposon insertions (from multi-insert lines): *F *F P{lacW}G0046a 3A1-4 *F P{lacW}G0046b 12F1-5 *F P{lacW}G0092a 17C1-4 *F P{lacW}G0092b 17D1-4 *F P{lacW}G0094a 4D1-2 *F P{lacW}G0094b 9E3-4 *F P{lacW}G0097a 4E *F P{lacW}G0097b 9C1-2 *F P{lacW}G0097c 12F1-3 *F P{lacW}G0137a 8F1-2 *F P{lacW}G0137b 9A1-3 *F P{lacW}G0147a 3A3 *F P{lacW}G0147b 18C1-2 *F P{lacW}G0161a 7E2-3 *F P{lacW}G0161b 7E5-8 *F P{lacW}G0199a 7D10-14 *F P{lacW}G0199b 18D10-E *F P{lacW}G0202a 10C1-2 *F P{lacW}G0202b 12B *F P{lacW}G0244a 4D1-2 *F P{lacW}G0244b 3A1-4 *F P{lacW}G0268a 2B13-18 *F P{lacW}G0268b 3A1-3 *F P{lacW}G0307a 7C7-9 *F P{lacW}G0307b 12F1-4 *F P{lacW}G0311a 3A1-4 *F P{lacW}G0311b 18C1-4 *F P{lacW}G0315a 3A1-4 *F P{lacW}G0315b 13F1-4 *F P{lacW}G0324a 3A1-4 *F P{lacW}G0324b 9E3-4 *F P{lacW}G0363a 18B1-4 *F P{lacW}G0363b 19E1-2 *F P{lacW}G0423a 2C *F P{lacW}G0423b 9E1-4 # *U FBrf0108530 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jun 28 18:48:25 1999 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Mon, 28 Jun 1999 17:46:04 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{w[+mC]=Ubi-GFP}61EF *F Mime-Version: 1.0 *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Subject: P{w[+mC]=Ubi-GFP}61EF *F *F In April 1999, David Bilder (Norbert Perrimon's lab, Harvard Medical *F School) sent stocks with an insertion of P{w[+mC]=Ubi-GFP} at 61EF on 3L *F which he named P{w[+mC]=Ubi-GFP}61EF. The insertion was isolated after *F mobilization of P{w[+mC]=Ubi-GFP}ID-1. *F *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0108531 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.28 *T personal communication to FlyBase *u *F *F From kcook@bio.indiana.edu Mon Jun 28 20:56:15 1999 *F X-Sender: kcook@sunflower.bio.indiana.edu (Unverified) *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Mon, 28 Jun 1999 19:53:55 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{w[+mC]=lacW}Mvl[97] *F Mime-Version: 1.0 *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Subject: P{w[+mC]=lacW}Mvl[97] *F *F In Rodrigues et al. 1995 (EMBO J. 14(13):3007-3020), the Mvl[97f] allele *F was said to be induced by mobilization of P elements from "C(1)RM(w[+])8". *F This corresponds the the attached-X chromosome in Bloomington stocks *F *F 16 C(1)RM, y[1] P{w[+mC]=lacW}5-45fD w[*] P{lacW}4-5fP P{lacW}3-52d *F P{lacW}3-76a/0/C(1;Y)13, v[1] f[1] *F *F 3697 C(1)RM, y[1] P{w[+mC]=lacW}5-45fD w[*] P{lacW}4-5fP P{lacW}3-52d *F P{lacW}3-76a/+ *F *F 3711 C(1)RM, y[1] P{w[+mC]=lacW}5-45fD w[*] P{lacW}4-5fP P{lacW}3-52d *F P{lacW}3-76a/w[1118]/Y *F *F Champakali Ayyub confirmed in correspondence (May 18, 1999) that the *F Mvl[97f] allele is an insertion of P{w[+mC]=lacW}. *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 *F *F # *U FBrf0108532 *a Strutt *b D. *t 1999.7.12 *T personal communication to FlyBase *u *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Subject: P{w[+mC]=Ubi-GFP} insertions *F *F The following information accompanied stocks donated by David Strutt, *F University of Sheffield (6/99): *F *F 1. Ilan Davis' original insertion identifer for P{w[+mC]=Ubi-GFP}ID-1 was *F 33C2. *F *F 2. Ilan Davis' original insertion identifer for P{w[+mC]=Ubi-GFP}33 was 34H. *F *F 3. Ilan Davis' original insertion identifer for P{w[+mC]=Ubi-GFP}38 was 34IL. *F # *U FBrf0108533 *a Knirr *b S. *t 1999.7.12 *T personal communication to FlyBase *u *F *F The following data was provided by *F *F From: stefan_knirr@smtplink.mssm.edu *F Date: Mon, 12 Jul 99 10:27:24 -0500 *F Stefan Knirr *F Mount Sinai Medical Center *F New York, NY 10029 *F *F In the insc allele insc-delta 13 the P-element is inserted after the 13th base *F pair of the transcriptional start site. The sequence in accession U73489 starts *F with base 1 of the transcription. *F *F *F ____________________________________________________________________ *F Subject: Where does the P8 insertion map in insc? *F From: Beverley Matthews at SMTP-for-MSSM *F Date: 6/21/99 1:31 PM *F *F Dear Dr. Knirr, *F *F I am a molecular curator for FlyBase and I am working on the annotation *F of the fly genome. I was hoping you could help with me a question about *F insc. *F *F In your 1997 Mechanisms of Development paper, it says that the insc[P8] *F insertion maps 13 bases downstream of the transcription start of insc. *F My problem is that I don't know where the transcription start is. Does *F the cDNA sequence in accession U73489 start with base 1 of the transcript? *F *F The start of the sequence is as follows: *F *F ATCAGAAGTC TGAGTCGAAG *F *F Is the site of insertion before or after the 13th base (an A) or *F somewhere else? If you can clarify this for me, I'll include it in the *F annotation along with the mapping of insc[delta13] and both will be *F referenced to your paper. *F *F Thanks for your help, *F *F Beverley Matthews *F # *U FBrf0108534 *a Lukacsovich *b T. *c Z. *d Asztalos *t 1999.7.15 *T personal communication to FlyBase *u *F Archived. # *U FBrf0108609 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Jun 2 17:33:26 1999 *F To: crosby@morgan.harvard.edu *F Subject: Kornberg GFP balancers *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: new GFP balancers *F Based on correspondence received by the Bloomington Stock Center, June 1999, *F from Thomas Kornberg and David Casso, UCSF: *F Stocks carrying GFP balancers (valid symbols not yet assigned): *F Df(1)JA27/FM7c, P{w+?=Kr-GAL4}'a', P{w+mC=UAS-GFP.S65T}'e'--line 10 *F Df(1)JA27/FM7c, P{w+?=Kr-GAL4}'a', P{w+mC=UAS-GFP.S65T}'f'--line34 *F w*; L* Pin*/CyO, P{w+?=Kr-GAL4}'c', P{w+mC=UAS-GFP.S65T}'g'--line 19 *F w*; L* Pin*/CyO, P{w+?=Kr-GAL4}'d', P{w+mC=UAS-GFP.S65T}'h'--line 30 *F y1 w*; D* gl3/TM3, Sb1, P{w+?=Kr-GAL4}'b', *F P{w+mC=UAS-GFP.S65T}'i'--line 4 *F y1 w*; D* gl3/TM3, Sb1, P{w+?=Kr-GAL4}'b', *F P{w+mC=UAS-GFP.S65T}'j'--line 5 *F Kr-GAL4 construct confirmed to be P{GAL4-Kr.C}. *F From crosby@morgan.harvard.edu Thu Jun 3 13:20:39 1999 *F To: kcook@bio.indiana.edu *F Subject: Re: Kornberg GFP balancers *F Insertions a == DC1 *F b == DC2 *F c == DC3 *F d == DC4 *F P{UAS-GFP.S65T} insertions: *F e == DC5 *F f == DC6 *F g == DC7 *F h == DC8 *F i == DC9 *F j == DC10 # *U FBrf0108610 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.21 *T personal communication to FlyBase *u *F From crosby@morgan.harvard.edu Mon Jun 21 19:13:47 1999 *F To: curators@morgan.harvard.edu *F Subject: lynn.updates.062199 *F Hi, Camcur *F I've been going through various subsets of insertions *F at Bloomington and came across the following insertions *F on balancers for which we do not seem to have balancer *F records: *F P1308 CyO, P{w+mC=CaSpeR}B26.2-36.1a P{CaSpeR}B26.2-36.1b *F P1310 CyO, P{w+mC=CaSpeR}B26.2-33.1a P{CaSpeR}B26.2-33.1b *F P1309 CyO, P{w+mC=CaSpeR}B26.2-16.1a P{CaSpeR}B26.2-16.1b *F P1312 CyO, P{w+mC=CaSpeR}B26.2-4.2a P{CaSpeR}B26.2-4.2b *F P974 CyO, P{ry+t7.2=lArB}A217.1M2 *F P947 CyO, P{ry+t7.2=lArB}A103.1M2 *F P1283 CyO, P{ry+t7.2=lArB}A130.1F2 *F Need a lacZ allele for P{lArB}58 (stock number P585) # *U FBrf0108611 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 22 02:58:33 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Wharton 59F8;60A7 complementation groups *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Wharton 59F8;60A7 complementation groups *F Stocks described in Wharton et al. 1999, Genetics 152: 629-640 were sent by *F Kristi Wharton to the Stock Center on June 21, 1999. The following allele *F information accompanied the stocks. Compare to Table 2 of paper. *F Complementation group Alleles Generated by *F l(2)60A-O 3-68 Wharton et al. *F 3-184 Wharton et al. *F l(2)60A-N ac5 Reed, 1992 *F Ac-3 Reed, 1992 *F Unassigned in Interval 2 Aq-3 Reed, 1992 *F Unassigned in Interval 2 At-3 Reed, 1992 *F Unassigned in Interval 2 4-173 Wharton et al. *F l(2)60A-K P8-61 Wharton et al. *F P18-110 Wharton et al. *F l(2)60A-L P4-111 Wharton et al. *F l(2)60A-C P20-180 Wharton et al. *F Ad-4 Reed, 1992 *F l(2)60A-A P14-73 Wharton et al. *F P18-132 Wharton et al. *F l(2)60A-I Ax-3 Reed, 1992 *F Al-4 Reed, 1992 *F ET/141 (=Unassigned in interval 5) 12-68 Wharton et al. *F Ac-4 Reed, 1992 *F l(2)60A-G Ax Reed, 1992 *F Ae-2 Reed, 1992 *F l(2)60A-E 3-106 Wharton et al. *F 2-30 Wharton et al. *F l(2)60A-B P14-200 Wharton et al. *F P17-30 Wharton et al. *F l(2)60A-H Ac-2 Reed, 1992 *F Ad-2 Reed, 1992 *F l(2)60A-D 2-198 Wharton et al. *F 3-109 Wharton et al. *F Reed, 1992 is FBrf0064297. *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0108612 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Jun 23 19:09:00 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 23 Jun 1999 19:09:00 +0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Wed, 23 Jun 1999 13:10:35 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Irick and Cherbas 71CE lethals *F Cc: lcherbas@sunflower.bio.INDIANA.edu *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Region 71CE complementation groups of Holly Irick and Peter Cherbas *F A screen for EMS-induced mutations that fail to complement Df(3L)BK10 was *F made by Holly Irick in Peter Cherbas' lab in 1988. All mutations were *F induced in a red1 e1 chromosome. None of the mutations corresponded to *F previously-identified complementation groups except for new mrn mutations. *F The following representative alleles were donated to the Stock Center on *F 6/23/99. *F Locus Alleles Lethal phase *F l(3)71CDa E3 embryonic or larval lethal *F E71 embryonic or larval lethal *F Deleted by Df(3L)878.3 *F l(3)71CDb E36 embryonic or larval lethal *F E65 embryonic or larval lethal *F Deleted by Df(3L)878.3 *F l(3)71CDc E7 embryonic or larval lethal *F E81 embryonic or larval lethal *F Deleted by Df(3L)878.3 *F l(3)71DEa E12 embryonic or larval lethal *F E39 embryonic or larval lethal *F Deleted by Df(3L)Brd6 and Df(3L)Brd18. Not deleted by Df(3L)878.3. *F l(3)71DEb E15 embryonic or larval lethal *F E55 embryonic or larval lethal *F Deleted by Df(3L)Brd6 and Df(3L)Brd18. Not deleted by Df(3L)878.3. *F l(3)71DEc E86 embryonic or larval lethal *F Deleted by Df(3L)Brd6 and Df(3L)Brd18. Not deleted by Df(3L)878.3. *F l(3)71DEd E59 pupal lethal *F Deleted by Df(3L)Brd6 and Df(3L)Brd18. Not deleted by Df(3L)878.3. *F l(3)71EFa E42 pupal lethal *F E51 pupal lethal *F Deleted by Df(3L)fz-M21. Not deleted by Df(3L)Brd6 or Df(3L)Brd18 *F mrn E67 *F Deleted by Df(3L)BK10. Not deleted by Df(3L)878.3. *F The following alleles of marionnette originated in this screen: E35 *F (FBal0012435), E67 (FBal0012436), E72 (FBal0012437), E94 (FBal0012438) and *F E96 (FBal0012439). *F ___________________________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology kcook@bio.indiana.edu *F Jordan Hall 142 812-855-5782 *F Indiana University 812-855-2577 (fax) *F 1001 E. Third St. *F Bloomington, IN 47405-3700 # *U FBrf0108613 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jun 28 23:20:01 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Region 95A-D mutations from Susan Abmayr *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Region 95A-D mutations from Susan Abmayr *F In November, 1998, Susan Abmayr sent representative alleles from lethal *F complementation groups in region 95A-D described in Erickson et al., 1997 *F J.Cell Biol. 138: 589-603. The alleles are: *F l(3)95Bal6.3 *F l(3)95BCaF6.11 *F l(3)95BCbF11.5 *F l(3)95BCcF10.1 *F l(3)95BCd339 *F l(3)95CaS5 *F mbcD11.2 # *U FBrf0108614 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jun 28 23:26:34 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Region 95A mutations from Susan Abmayr *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Region 95A mutations from Susan Abmayr *F In November, 1998, Susan Abmayr sent representative EMS alleles from lethal *F complementation groups in region 95AB described in Keller et al., 1998 Dev *F Biol. 202: 157-171. The alleles are: *F l(3)95AxA1 *F l(3)95AyF1.19 *F l(3)95AzE2.33 *F l(3)01152C14.5 *F l(3)04684C14.4 # *U FBrf0108615 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 29 01:41:41 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Taf601 *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Taf60 allele from Jim Kennison *F In February, 1999, we received a stock of the EMS-induced Taf601 allele *F from Jim Kennison. It was isolated in a screen for lethals in region 76. # *U FBrf0108616 *a Bloomington Drosophila Stock Center *b ?. *t 1999.7.1 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 29 01:52:16 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{w+mC=lacW}Mvl97 *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: P{w+mC=lacW}Mvl97 *F In Rodrigues et al. 1995 (EMBO J. 14(13):3007-3020), the Mvl97f allele *F was said to be induced by mobilization of P elements from 'C(1)RM(w+)8'. *F This corresponds the the attached-X chromosome in Bloomington stocks *F 16 C(1)RM, y1 P{w+mC=lacW}5-45fD w* P{lacW}4-5fP P{lacW}3-52d *F P{lacW}3-76a/0/C(1;Y)13, v1 f1 *F 3697 C(1)RM, y1 P{w+mC=lacW}5-45fD w* P{lacW}4-5fP P{lacW}3-52d *F P{lacW}3-76a/+ *F 3711 C(1)RM, y1 P{w+mC=lacW}5-45fD w* P{lacW}4-5fP P{lacW}3-52d *F P{lacW}3-76a/w1118/Y *F Champakali Ayyub confirmed in correspondence (May 18, 1999) that the *F Mvl97f allele is an insertion of P{w+mC=lacW}. # *U FBrf0108617 *a Bloomington Drosophila Stock Center *b ?. *t 1999.7.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 29 16:14:28 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Karp and Cherbas deletions and point mutations *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Karp and Cherbas deletions and complementation groups in region 55-56 *F In 1984-85, Robert Karp in Peter Cherbas' lab generated deletions in the *F region of Eip55E by X ray treatment of Tp(1;2)TE55Ea. Three of the *F deletions recovered (with Karp's cytology) were *F Df(2R)Pu29 55C1,2;56B1,2 *F Df(2R)Pu66 55D2-4;55E4 *F Df(2R)Pu34 55D2-E1;56B2 *F Subsequently, Karp and Cherbas screened for lethal complementation groups *F that failed to complement Df(2R)Pu34. They defined five lethal *F complementation groups (A through E) with 2 to 11 EMS-induced alleles per *F group. Alleles include: *F Complementation group Allele *F A EMS38 *F EMS93 *F B EMS44 *F EMS58 *F C EMS27 *F EMS69 *F D EMS67 *F EMS104 *F E EMS81a *F EMS95 *F This information accompanied stocks donated to the Stock Center 6/29/99 by *F Peter Cherbas and Lucy Cherbas. # *U FBrf0108618 *a Bloomington Drosophila Stock Center *b ?. *t 1999.7.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 29 16:27:19 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Irick and Cherbas Ly region complementation groups *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: Ly region complementation groups of Holly Irick and Peter Cherbas *F A screen for EMS-induced mutations that fail to complement Df(3L)BK10 was *F made by Holly Irick in Peter Cherbas' lab in 1988. The Df(3L)BK10 *F chromosome was marked with Ly1 (=Df(3L)Ly); consequently, two lethal *F complementation groups were defined in the 70A region which Irick and *F Cherbas called Ly-1 and Ly-2. Alleles include: *F Complementation group Allele *F Ly-1 E1 *F E2 *F E53 *F E54 *F E58 *F E64 *F E69 *F Ly-2 E70 *F All mutations were induced in a red1 e1 chromosome. *F This information accompanied stocks donated to the Stock Center on 6/29/99 *F by Peter Cherbas and Lucy Cherbas. # *U FBrf0108619 *a Bloomington Drosophila Stock Center *b ?. *t 1999.6.20 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: TM2Z *F Date: 20 June 1999 *F *F Information communicated: *F *F Bloomington stock records indicate that the balancer TM2Z carries a mutant allele of ry. The specific mutant allele is unknown. The balancer short genotype is therefore TM2, ry[*] P{lArB}C40.1S3. # *U FBrf0108818 *a Johansen *b K. *t 1999.6.3 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Jun 03 16:16:07 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 3 Jun 1999 16:16:07 +0100 *F To: kristen@iastate.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 3 Jun 1999 16:19:53 +0100 *F Content-Length: 979 *F Dear Dr. Johansen, *F I am curating your paper for FlyBase: *F Walker et al., 1998, Molec. Biol. Cell 9(Suppl.): 445a *F 'Skeletor: An essential novel nucleoskeletal protein that forms a *F unique spindle early in cell division.' *F You mention that Skeletor was originally identified using the antibody *F mAb2A. *F In a previous reference (Jin et al., 1997, A. Conf. Dros. Res. 38: *F 168B) you state that antibody mAb2A recognises 2 antigens: JIL-1 and *F 2Ab3. *F I would be grateful if you could confirm whether Skeletor corresponds *F to one of these antigens, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From kristen@iastate.edu Thu Jun 03 16:38:35 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 3 Jun 1999 16:38:35 +0100 *F Mime-Version: 1.0 *F X-Sender: kristen@POP-1.IASTATE.EDU *F Date: Thu, 3 Jun 1999 10:42:16 -0500 *F To: Gillian Millburn (Genetics) *F From: Kristen Johansen *F Subject: Re: FlyBase query *F >Dear Dr. Johansen, *F > *F >I am curating your paper for FlyBase: *F > *F >Walker et al., 1998, Molec. Biol. Cell 9(Suppl.): 445a *F > *F >'Skeletor: An essential novel nucleoskeletal protein that forms a *F >unique spindle early in cell division.' *F > *F >You mention that Skeletor was originally identified using the antibody *F >mAb2A. *F > *F >In a previous reference (Jin et al., 1997, A. Conf. Dros. Res. 38: *F >168B) you state that antibody mAb2A recognises 2 antigens: JIL-1 and *F >2Ab3. *F > *F >I would be grateful if you could confirm whether Skeletor corresponds *F >to one of these antigens, *F > *F >I look forward to hearing from you, *F > *F >Gillian *F Dear Gillian, *F Yes, Skeletor is the antigen corresponding to 2Ab3 in the abstract you *F describe above. Another citation link is the paper Johansen (1996) J. Cell. *F Biochem. 60: 289-296, which mentions that mAb2A identified an antigen *F temporarily designated as 2Ab3, but we hadn't named it Skeletor at that *F point. *F There will be a paper in Molecular Cell next month reporting the JIL-1 *F antigen (the other of the mAb2A antigens), but I assume that will get *F organized with this information at a later time! *F Let me know if you need any other information, and thanks for your *F assistance with FlyBase. *F Best regards, *F Kristen Johansen *F Kristen M. Johansen, Ph.D. *F Associate Professor of Zoology and Genetics *F 3154 Molecular Biology Building *F Iowa State University *F Ames, Iowa 50011 *F phone:515-294-7959 *F fax: 515-294-0345 *F e-mail: kristen@iastate.edu # *U FBrf0108830 *a Kaufman *b T. *t 1999.6.7 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Mon Jun 07 15:40:28 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 7 Jun 1999 15:40:28 +0100 *F To: kaufman@bio.indiana.edu *F Subject: cnn query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 7 Jun 1999 15:44:05 +0100 *F Content-Length: 2094 *F Hi Thom, *F I am curating your centrosomin paper: *F Megraw et al., 1999, Development 126(13): 2829--2839 *F you mention at the beginning of the results that mutations *F previously reported to be in cnn (Li and Kaufman, 1996, Cell 85(4): *F 585--596) have since been determined to be in arrow. I thought I'd *F check that we have this right as I know there's been a problem with arr *F and cnn and sadly, all the alleles in this paper are under cnn and not *F arr. *F here are the 'cnn' alleles from that paper: *F \*A cnn1 *F \*A cnn4 *F \*A cnn12-1 *F \*A cnn16-1 *F can you confirm that they are all in fact alleles of arrow (I'll make *F that info a pc from you if that's the case) *F If the 4 above are arrow alleles, I'll have to make what is currently *F 'cnn1' something like ' arrL1' (L for Li) as there is already an *F arr1 in the database. Is that OK ? *F Gillian *F From kaufman@sunflower.bio.indiana.edu Mon Jun 07 17:40:13 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 7 Jun 1999 17:40:13 +0100 *F Mime-Version: 1.0 *F Date: Mon, 7 Jun 1999 11:44:04 -0500 *F To: Gillian Millburn (Genetics) *F From: kaufman@bio.indiana.edu (T. Kaufman) *F Subject: Re: cnn query *F Yes all of the things we called cnn in that paper are indeed really *F arr. The paper you are now curating has reported in it the only bona *F fide alleles of cnn. cnn does not mutate to9 lethality. *F \--tk *F Thom Kaufman kaufman@sunflower.bio.indiana.edu *F \--- Biology Dept., Indiana University, Bloomington, IN 47405 --- *F 812-855-3033/Office--812-855-7674/Lab--812-855-2577/FAX # *U FBrf0108925 *a McKim *b K. *t 1999.6.30 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Kim McKim, Rutgers University *F To: Bloomington Drosophila Stock Center *F Subject: f[K36] *F Dated: 1 November 1998 *F *F Background: forked allele in a Bloomington stock that is not in FlyBase *F *F Information communicated: *F *F f[K36], X-ray induced on Dp(1;4)r[+] (it is not known whether this was the long, medium or short variant of Dp(1;4)r[+]) # *U FBrf0109002 *a Philp *b A. *t 1999.7.14 *T personal communication to FlyBase *u *F From ajvphilp@scotlandmail.com Fri Jul 02 20:20:02 1999 *F To: rd120@gen.cam.ac.uk *F Subject: pollyanna and free thinker *F i fear the flybase entry on the gene pana will cause confusion. *F pollyanna (pana) and thag are not the same gene. the gene that was *F originally named free thinker was renamed pollyanna after we found that *F mutations in it elevate the number of anaphases. the gene that was *F originally called (in our original GSA abstract although not on the *F presented poster) think again (thag) was renamed (unfortunately) free *F thinker. free thinker mutants have chromosome alignment defects. *F . *F . *F please contact me again if you require further clarification. *F alastair valentine philp # *U FBrf0109036 *a Roote *b J. *t 1999.6.8 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Tue Jun 08 18:12:40 1999 *F To: rd120@mole.bio.cam.ac.uk *F Subject: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 *F Rachel, *F Another note for FlyBase: *F From the cytology one would predict that *F Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 was a deletion from the GT6 breakpoint *F to TE35B i.e. Df(2L)34F3;35B1.2. Genetically it should be deleted for wb, *F ms(2)34Fe, l(2)34Fc, l(2)34Fd, l(2)35Aa, el, pu and noc. *F In fact it is hypomorphic for wb, l(2)34Fc, l(2)34Fd, l(2)35Aa and *F wild-type for pu. *F Over el1 it is phenotypically el. *F Over ms(2)34Fe1715 it is male fertile - but hard to score degrees of male *F fertileness. *F I got a similar result from the strange segregant *F Ts(2Lt;4Lt)GT6+Ts(2Rt;Y)TE35B-18. *F GT6 itself is hypomorphic for wb. *F Therefore I assume that GT6 is a complex event which carries a *F heterochromatic duplication of wb-pu. *F John *F From rd120@gen.cam.ac.uk Wed Jun 09 09:58:16 1999 *F To: rd120@mole.bio.cam.ac.uk, j.roote@gen.cam.ac.uk *F Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 *F Thanks John, *F I'll curate your message as a pc from you to us - The fact that you *F get consistent results in your complementation tests suggests that the *F heterochromatic duplication is into the bit of the 4th that is included *F in Ts(2Lt;4Lt)GT6, doesn't it? *F Has anybody mapped the breaks molecularly? *F Rach *F From j.roote@gen.cam.ac.uk Wed Jun 09 12:04:33 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 *F > The fact that you get consistent results in your *F >complementation tests suggests that the heterochromatic duplication is *F >into the bit of the 4th that is included in Ts(2Lt;4Lt)GT6, doesn't it? *F Yes, indeed it does - well spotted. *F >Has anybody mapped the breaks molecularly? *F Yes. Terry D. will send me his best guess for this. *F Rootles *F From rd120@gen.cam.ac.uk Wed Jun 09 17:28:35 1999 *F To: j.roote@gen.cam.ac.uk *F Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 *F Hi John, *F me again. *F Have you, by any chance, complementation tested T(2;4)GT6 itself for *F hypomorphic-ness at l(2)34Fc, l(2)34Fd, l(2)35Aa? *F Rach *F From j.roote@gen.cam.ac.uk Wed Jun 09 18:03:59 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 *F Hiya, *F me again. *F >Have you, by any chance, complementation tested T(2;4)GT6 itself for *F >hypomorphic-ness at l(2)34Fc, l(2)34Fd, l(2)35Aa? *F I have - T(2;4)GT6 is hypomorphic for wb and nothing else. *F I think it must involve extra breaks. T(2;4)GT6 is broken (cytologically, *F molecularly and genetically) somewhere near wb. *F This putative variegating Dp only becomes 'noticeable' in the absence of *F Ts(2Rt;4Rt)GT6 (or should I say in the absence of 34Fc, 34Fd, etc.). *F J # *U FBrf0109185 *a Bloomington Drosophila Stock Center *b ?. *t 1999.7.5 *T personal communication to FlyBase *u *F Bloomington Stock Center records, July 1999. *F Subset of lacZ enhancer trap lines. *F *F >>> *F B-#P546 w[1118] sev[14]; P{w[+mW.hs]=sev2}ch21 *F Chromosome(s) affected: 2 *F Insertion site: 046E *F Also known as: T ch21 *F Date added: 5/01/91 *F Donor: Ernst Hafen *F Comments: Hafen #15; first short exon of sev cDNA only; rescues when heat *F shocked; eye-color: red *F Center: Bloomington Drosophila Stock Center Contact: flystocks@bio.indiana.edu *F *F >>> *F B-#P693 y[*] w[*]; P{w[+mC]=lacW}B9-3-25 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: note with stocks said this insert is on 2 despite the acquisition *F nomenclature, K.M. *F Embryo-Jan: sensilla (stage 13), gut (stage 9), somatic muscle (stage 9), *F visceral muscle (stage 9), dorsal vessel (stage 12) *F Adult Brain-Vosshall: fat body, optic lobe *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P694 y[*] w[*]; P{w[+mC]=lacW}B9-3-40 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), somatic muscle (stage 11), dorsal vessel *F (stage 13) *F Adult Brain-Vosshall: lamina, 3rd antennal segment *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P695 y[*] w[*]; P{w[+mC]=lacW}B9-3-52 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 7), sensilla (stage 17), dorsal vessel (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P697 y[*] w[*]; P{w[+mC]=lacW}B10-2-3 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: gonad sheath (stage 15) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P696 y[*] w[*]; P{w[+mC]=lacW}l(3)B9-3-53[1]/TM3 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), trachea (stage 13), gut (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F *F >>> *F B-#P699 y[*] w[*]; P{w[+mC]=lacW}B11-3-6 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 14), sensilla (stage 14) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P700 y[*] w[*]; P{w[+mC]=lacW}B11-3-11 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P702 y[*] w[*]; P{w[+mC]=lacW}B12-3-5 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), CNS (stage 11), gut *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P705 y[*] w[*]; P{w[+mC]=lacW}C2-2-26 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 14) *F Adult Brain-Vosshall: medulla *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P706 y[*] w[*]; P{w[+mC]=lacW}C3-2-2 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 13), gut, visceral muscle, oenocytes *F Adult Brain-Vosshall: 3rd antennal segment, central brain *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P708 y[*] w[*]; P{w[+mC]=lacW}C3-2-21 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS, gut, dorsal vessel, oenocytes *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P709 y[*] w[*]; P{w[+mC]=lacW}C3-3-30 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 13), CNS (stage 13), gut (stage 13) *F Adult Brain-Vosshall: discrete optic lobe *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P710 y[*] w[*]; P{w[+mC]=lacW}C3-3-36 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: trachea (stage 13), gonad sheath (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P727 y[*] w[*]; P{w[+mC]=lacW}C4-3-20 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 12), CNS (stage 13), sensilla (stage 13), *F gonad sheath *F Adult Brain-Vosshall: optic lobe *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P729 y[*] w[*]; P{w[+mC]=lacW}C5-2-7 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 12), CNS (stage 13), gut (stage 15), *F visceral muscle (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F *F >>> *F B-#P730 y[*] w[*]; P{w[+mC]=lacW}C5-3-22 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), somatic muscle (stage 12), dorsal vessel *F (stage 13), hemocytes *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F *F >>> *F B-#P732 y[*] w[*]; P{w[+mC]=lacW}C6-2-36 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: CyO floating, K.M. 1/27/95 *F Embryo-Jan: CNS (stage 14), gonad sheath (stage 14) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P734 y[*] w[*]; P{w[+mC]=lacW}C6-3-9 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: note with stocks says insert is on 2, despite the acquisition *F nomenclature *F Embryo-Jan: CNS *F Adult Brain-Vosshall: optic lobe, central brain, 2nd antennal segment *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P735 y[*] w[*]; P{w[+mC]=lacW}C7-3-34 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis, CNS (stage 14) *F Adult Brain-Vosshall: eye, optic lobe, central brain *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P737 y[*] w[*]; P{w[+mC]=lacW}C8-3-5 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 12) *F Adult Brain-Vosshall: discrete central brain, 3rd antennal segment *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P746 y[*] w[*]; P{w[+mC]=lacW}C8-3-37 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), trachea, degenerating cells (stage 12) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P747 y[*] w[*]; P{w[+mC]=lacW}D3-3-11 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 7), CNS (stage 9), visceral muscle (stage *F 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P748 y[*] w[*]; P{w[+mC]=lacW}E1-2-32 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 12), sensilla (stage 13), trachea (stage 12) *F Adult Brain-Vosshall: optic lobe, central brain, 2nd antennal segment *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P749 y[*] w[*]; P{w[+mC]=lacW}E1-2-38 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 12), CNS (stage 13), visceral muscle (stage *F 12) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P750 y[*] w[*]; P{w[+mC]=lacW}E2-3-27 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 11), sensilla (stage 12), gut (stage 11), somatic *F muscle (muscle pioneers?, stage 12), histoblasts *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P752 y[*] w[*]; P{w[+mC]=lacW}E6-2-17 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), chordotonal organs? *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P753 y[*] w[*]; P{w[+mC]=lacW}E6-3-7/TM3 *F Chromosome(s) affected: 3 *F Insertion site: 086E *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: CNS (stage 12), sensilla (stage 13), gut, garland cells *F Adult Brain-Vosshall: medulla, central brain *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P755 y[*] w[*]; P{w[+mC]=lacW}E6-3-50 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P761 y[*] w[*]; P{w[+mC]=lacW}howE7-3-4 *F Chromosome(s) affected: 3 *F Insertion site: 093F13 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS, somatic muscle (stage 11), visceral muscle (stage 11), *F dorsal vessel (stage 11), outer sheath of midgut *F Adult Brain-Vosshall: proboscis *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P762 y[*] w[*]; P{w[+mC]=lacW}E7-3-33/TM3 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: CNS (stage 13), hemocytes *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P773 y[*] w[*]; P{w[+mC]=lacW}E7-3-63 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: epidermis (stage 11), CNS (stage 13), dorsal vessel (stage *F 11), fat body, triplet cells (unknown structure, stage 12) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P776 y[*] w[*]; P{w[+mC]=lacW}E8-2-18 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: hemocytes (stage 12) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F *F >>> *F B-#P778 y[*] w[*]; P{w[+mC]=lacW}l(3)E8-3-54[1]/TM3 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 15), trachea (stage 13), gut (stage 15) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P779 y[*] w[*]; P{w[+mC]=lacW}E8-3-57 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), somatic muscle (stage 13), dorsal vessel *F (stage 13), gonad sheath, dorsal fat body *F Adult Brain-Vosshall: optic lobe *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P780 y[*] w[*]; P{w[+mC]=lacW}E8-3-63 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 10), trachea (stage 11) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F *F >>> *F B-#P782 y[*] w[*]; P{w[+mC]=lacW}E9-2-2 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: TM3 floating, K.M. 1/27/95 *F Embryo-Jan: CNS (stage 11) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P789 y[*] w[*]; P{w[+mC]=lacW}E9-3-31 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), gut (stage 15), dorsal vessel (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P792 y[*] w[*]; P{w[+mC]=lacW}E10-2-26 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), amnioserosa *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P822 y[*] w[*]; P{w[+mC]=lacW}E10-2-41 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P823 y[*] w[*]; P{w[+mC]=lacW}E10-3-1 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 10), CNS (stage 12) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P825 y[*] w[*]; P{w[+mC]=lacW}E10-3-40 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 12), sensilla (stage 12) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P826 y[*] w[*]; P{w[+mC]=lacW}l(2)A1-2-12[1]/CyO *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), sensilla (stage 12), trachea (stage 11) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P828 y[*] w[*]; P{w[+mC]=lacW}A1-2-54 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: Chin Chiang says there are two elements present in this stock *F Embryo-Jan: epidermis (stage 10), CNS (stage 10), trachea (stage 10), gut *F (stage 10) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P829 y[*] w[*]; P{w[+mC]=lacW}A1-3-1 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 9), CNS (stage 8), gut (stage 3), visceral *F muscle (stage 11) *F Adult Brain-Vosshall: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F >>> *F B-#P837 y[*] w[*]; P{w[+mC]=lacW}A1-3-10 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 12), trachea (stage 11), gut *F Adult Brain-Vosshall: central brain *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F Vosshall == Leslie Vosshall *F *F *F >>> *F B-#P843 y[*] w[*]; P{w[+mC]=lacW}A3-3-25 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 12), gut *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P846 y[*] w[*]; P{w[+mC]=lacW}A3-3-47 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 13), trachea (stage 13), gut (stage 13) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P849 y[*] w[*]; P{w[+mC]=lacW}A3-3-61 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 12), CNS, trachea (stage 12), gut (stage *F 12), visceral muscle (stage 12), dorsal vessel (stage 13) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P867 y[*] w[*]; P{w[+mC]=lacW}A4-2-5 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: somatic muscle (stage 13) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P868 y[*] w[*]; P{w[+mC]=lacW}A4-3-40 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), sensilla (lateral chordotonal organ, *F stage 12) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P871 y[*] w[*]; P{w[+mC]=lacW}A4-3-53 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), CNS (stage 13), sensilla (stage 11), *F transverse fiber cells (unknown structure) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P873 y[*] w[*]; P{w[+mC]=lacW}A5-2-6 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), CNS (stage 13), amnioserosa *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P888 y[*] w[*]; P{w[+mC]=lacW}A6-3-2 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 12), gut (stage 12), somatic muscle (stage 12) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P889 y[*] w[*]; P{w[+mC]=lacW}A6-3-11 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 11), CNS (stage 13) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P890 y[*] w[*]; P{w[+mC]=lacW}l(3)A6-3-56[1]/TM3 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 15), somatic muscle (stage 15), dorsal vessel *F (stage 15), oenocytes (stage 15) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P891 y[*] w[*]; P{w[+mC]=lacW}A6-3-69 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 14), sensilla (stage 14), trachea (stage 14) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P892 y[*] w[*]; P{w[+mC]=lacW}B1-3-40 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 11), visceral muscle (stage 11) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P893 y[*] w[*]; P{w[+mC]=lacW}B1-3-49 *F Chromosome(s) affected: 3? *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: insert may be on 2 *F Embryo-Jan: epidermis (stage 13), CNS (stage 13), gut (stage 13), *F visceral muscle (stage 13), dorsal fat body (stage 15) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P895 y[*] w[*]; P{w[+mC]=lacW}B3-2-32 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 12), trachea (stage 13), somatic muscle (stage *F 12), dorsal fat body (stage 12) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P898 y[*] w[*]; P{w[+mC]=lacW}B4-2-27 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: CyO floating, K.M. 1/27/95 *F Embryo-Jan: epidermis (stage 15), trachea (stage 15), somatic muscle *F (stage 14), oenocytes (stage 12) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F *F >>> *F B-#P899 y[*] w[*]; P{w[+mC]=lacW}B4-3-8 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 13), sensilla (stage 16) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P900 y[*] w[*]; P{w[+mC]=lacW}l(3)B4-3-20[1]/TM3 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS (stage 13), triplet cells (unknown structure) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P901 y[*] w[*]; P{w[+mC]=lacW}B4-3-31 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: epidermis (stage 14), sensilla (stage 14), trachea (stage *F 14), gut (stage 14) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P903 y[*] w[*]; P{w[+mC]=lacW}B6-2-30 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: somatic muscle (stage 12), visceral muscle (stage 12) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P904 y[*] w[*]; P{w[+mC]=lacW}B6-3-18 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: visceral muscle (stage 11), gonad sheath *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P905 y[*] w[*]; P{w[+mC]=lacW}B6-3-23 *F Chromosome(s) affected: 3 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: somatic muscle (stage 13) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P927 y[*] w[*]; P{w[+mC]=lacW}B7-2-22 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Embryo-Jan: CNS, trachea, gut, somatic muscle *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P934 y[*] w[*]; P{w[+mC]=lacW}B9-2-33 *F Chromosome(s) affected: 2 *F Date added: 8/01/92 *F Donor: Y. N. Jan *F Comments: possibly female sterile *F Embryo-Jan: somatic muscle (stage 14) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Jan == Volker Hartenstein & Juh Nung Jan *F *F >>> *F B-#P957 P{ry[+t7.2]=lArB}A154.2M3, ry[506] *F Chromosome(s) affected: 3 *F Also known as: #WG957 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: malpighian tubules, head *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1129 P{ry[+t7.2]=lArB}A355.1F3, ry[506] *F Chromosome(s) affected: 3 *F Also known as: #WG1129 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: amnioserosa, heart *F Adult Ovary-Bellen: germarium (region 2), nurse cells (stage 8), oocyte *F nucleus (stage 10B), ooplasm (stage 13) *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1165 P{ry[+t7.2]=lArB}A289.1F1; ry[506] *F Chromosome(s) affected: 1 *F Also known as: #WG1165 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: malpighian tubules, head, CNS subset, PNS *F Adult Ovary-Bellen: germarium, follicle cells, border cells *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1210 P{ry[+t7.2]=lArB}A308.1M3, ry[506] *F Chromosome(s) affected: 3 *F Also known as: #WG1210 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: amnioserosa, heart, head, hindgut, CNS subset *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F *F >>> *F B-#P1296 P{ry[+t7.2]=lArB}A229.1F1; ry[506] *F Chromosome(s) affected: 1 *F Also known as: #WG1296 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Comments: good marker for mitotic recombination due to uniform staining in *F imaginal and pupal tissues (Seth Blair) *F Larva-Bellen: brain, all discs, uniform *F Pupa-Blair: uniform *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F Blair == Seth Blair *F *F >>> *F B-#P1460 P{ry[+t7.2]=lArB}A487.1M3, ry[506] *F Chromosome(s) affected: 3 *F Also known as: #WG1460 *F Date added: 8/01/90 *F Donor: HHMI P Stock Center *F Donor's source: Walter Gehring *F Embryo-Bellen: muscle *F Adult Ovary-Bellen: no staining *F Center: Bloomington Stock Center Contact: flystocks@bio.indiana.edu *F Source(s) of pattern information: *F Bellen == Hugo Bellen *F # *U FBrf0109186 *a Micklem *b D. *t 1999.7.22 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase, 22 July 1999, from David Micklem, *F Dept. of Biochemistry, Stanford University, Stanford, CA, USA. *F *F Corrections and additions to compiled sequence of pPacPL. *F *F *F LOCUS PPAC-PL.GE 6429 BP SS-DNA CIRCULAR SYN 07-JAN-1993 *F DEFINITION pPacPL Vector for transient expression in Drosophila cell cultures *F ACCESSION - *F KEYWORDS - *F SOURCE Synthetic construct DNA. *F ORGANISM synthetic construct artificial sequence. *F FEATURES Location/Qualifiers *F frag 2612..2986 *F /note="Confirmed by sequencing" *F frag 4905..5316 *F /note="Confirmed by sequencing" *F source 1..2400 *F /note="/segment=""pUC18(S) (GB/L08752 287..2686)""" *F source 2401..2628 *F /note="/segment=""pUC18(N) (GB/L08752 1..229)""" *F source 2629..2634 *F /note="/segment=""J EcoRI""" *F source 2635..2662 *F /note="/segment=""Act5C_5' (R1,u)0.2 - now sequenced, and *F is only 27bp, not 200bp""" *F source 2663..4595 *F /note="/segment=""Act5C_P2.45 (GB/X15730 1..????)"" with *F minor changes from DRM sequencing" *F source 4596..4599 *F /note="/segment=""J filled in SpeI site"" (not in original *F flybase sequence)" *F source 4600..5121 *F /note="/segment=""Act5C_P2.45 (GB/X15730 *F ????..2449)"" " *F source 5122..5209 *F /note="/segment=""Act5C+0.088 (GB/X06382 205..292)""" *F source 5210..5274 *F /note="/segment=""J pacPL.mcs now sequenced" *F source 5275..6386 *F /note="=""Act5C+1.1(S) (GB/X06384 7..1118)"" " *F source 6387..6429 *F /note="/segment=""J 001193""" *F unsure 5164..5164 *F /note="C or G Both variants are present in the Krasnow *F lab. Avoid using this region for sequencing primers." *F unsure 3047..3047 *F /note="This A probably present(not in old flybase sequence *F but my sequence quality marginal)" *F variation 2892..2892 *F /note="Additional G (vs old flybase sequence)" *F variation 2853..2854 *F /note="Additional CA (vs old flybase sequence)" *F variation 2842..2842 *F /note="G (vs C in old flybase sequence)" *F variation 2840..2840 *F /note="Additional A (vs old flybase sequence)" *F variation 2664..2664 *F /note="Additional A (vs old flybase sequence)" *F variation 2620..2620 *F /note="Only single C (vs CC in old flybase sequence)" *F BASE COUNT 1814 A 1494 C 1442 G 1679 T 0 OTHER *F ORIGIN - *F 1 GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA *F 61 TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA *F 121 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT *F 181 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC *F 241 TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG *F 301 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT *F 361 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG *F 421 CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT *F 481 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA *F 541 TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT *F 601 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT *F 661 TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG *F 721 AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA *F 781 AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG *F 841 TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT *F 901 TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG *F 961 CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG *F 1021 AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA *F 1081 TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC *F 1141 TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC *F 1201 CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC *F 1261 GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG *F 1321 CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC *F 1381 GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC *F 1441 ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT *F 1501 AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC *F 1561 CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA *F 1621 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC *F 1681 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT *F 1741 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG *F 1801 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC *F 1861 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT *F 1921 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA *F 1981 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT *F 2041 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG *F 2101 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA *F 2161 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA *F 2221 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT *F 2281 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA *F 2341 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA *F 2401 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA *F 2461 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT *F 2521 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT *F 2581 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTCTATATT *F 2641 CTAAAAACAC AAATGATACT TCTAAAAAAA AATCATGAAT GGCATCAACT CTGAATCAAA *F 2701 TCTTTGCAGA TGCACCTACT TCTCATTTCC ACTGTCACAT CATTTTTCCA GATCTCGCTG *F 2761 CCTGTTATGT GGCCCACAAA CCAAGACACG TTTTATGGCC ATTAAAGCTG GCTGATCGTC *F 2821 GCCAAACACC AAATACATAA TGAATATGTA CACATTCGAG AAAGAAGCGA TCAAAGAAGC *F 2881 GTCTTCGGGC GGAGTAGGAG AATGCGGAGG AGAAGGAGAA CGAGCTGATC TAGTATCTCT *F 2941 CCACAATCCA ATGCCAACTG ACCAACTGGC CATATTCGGA GCAATTTGAA GCCAATTTCC *F 3001 ATCGCCTGGC GATCGCTCCA TTCTTGGCTA TATGTTTTTC ACCGTTACCC GGGGCCATTT *F 3061 TCAAAGACTC GTCGGCAAGA TAAGATTGTG TCACTCGCTG TCTCTCTTCA TTTGTCGAAG *F 3121 AATGCTGAGG AATTTCGCGA TGACGTCGGC GAGTATTTTG AAGAATGAGA ATAATTTGTA *F 3181 TTTATACGAA AATCAGTTAG TGGAATTTTC TACAAAAACA TGTTATCTAT AGATAATTTT *F 3241 GTTGCAAAAT ATGTTGACTA TGACAAAGAT TGTATGTATA TACCTTTAAT GTATTCTCAT *F 3301 TTTCTTATGT ATTTATAATG GCAATGATGA TACTGATGAT ATTTTAAGAT GATGCCAGAC *F 3361 CACAGGCTGA TTTCTGCGTC TTTTGCCGAA CGCAGTGCAT GTGCGGTTGT TGTTTTTTGG *F 3421 AATAGTTTCA ATTTTCGGAC TGTCCGCTTT GATTTCAGTT TCTTGGCTTA TTCAAAAAGC *F 3481 AAAGTAAAGC CAAAAAAGCG AGATGGCAAT ACCAAATGCG GCAAAACGGT AGTGGAAGGA *F 3541 AAGGGGTGCG GGGCAGCGGA AGGAAGGGTG GGGCGGGGCG TGGCGGGGTC TGTGGCTGGG *F 3601 CGCGACGTCA CCGACGTTGG AGCCACTCCT TTGACCATGT GTGCGTGTGT GTATTATTCG *F 3661 TGTCTCGCCA CTCGCCGGTT GTTTTTTTCT TTTTATCTCG CTCTCTCTAG CGCCATCTCG *F 3721 TACGCATGCT CAACGCACCG CATGTTGCCG TGTCCTTTAT GCGTCATTTT GGCTCGAAAT *F 3781 AGGCAATTAT TTAAACAAAG ATTAGTCAAC GAAAACGCTA AAATAAATAA GTCTACAATA *F 3841 TGGTTACTTA TTGCCATGTG TGTGCAGCCA ACGATAGCAA CAAAAGCAAC AACACAGTGG *F 3901 CTTTCCCTCT TTCACTTTTT GTTTGCAAGC GCGTGCGAGC AAGACGGCAC GACCGGCAAA *F 3961 CGCAATTACG CTGACAAAGA GCAGACGAAG TTTTGGCCGA AAAACATCAA GGCGCCTGAT *F 4021 ACGAATGCAT TTGCAATAAC AATTGCGATA TTTAATATTG TTTATGAAGC TGTTTGACTT *F 4081 CAAAACACAC AAAAAAAAAA ATAAAACAAA TTATTTGAAA GAGAATTAGG AATCGGACAG *F 4141 CTTATCGTTA CGGGCTAACA GCACACCGAG ACGAAATAGC TTACCTGACG TCACAGCCTC *F 4201 TGGAAGAACT GCCGCCAAGC AGACGATGCA GAGGACGACA CATAGAGTAG CGGAGTAGGC *F 4261 CAGCGTAGTA CGCATGTGCT TGTGTGTGAG GCGTCTCTCT CTTCGTCTCC TGTTTGCGCA *F 4321 AACGCATAGA CTGCACTGAG AAAATCGATT ACCTATTTTT TATGAATGAA TATTTGCACT *F 4381 ATTACTATTC AAAACTATTA AGATAGCAAT CACATTCAAT AGCCAAATAC TATACCACCT *F 4441 GAGCGATGCA ACGAAATGAT CAATTTGAGC AAAAATGCTG CATATTTAGG ACGGCATCAT *F 4501 TATAGAAATG CTTCTTGCTG TGTACTTTTC TCTCGTCTGG CAGCTGTTTC GCCGTTATTG *F 4561 TTAAAACCGG CTTAAGTTAG GTGTGTTTTC TACGACTAGC TAGTGATGCC CCTACTAGAA *F 4621 GATGTGTGTT GCACAAATGT CCCTGAATAA CCAATTTGAA GTGCAGATAG CAGTAAACGT *F 4681 AAGCTAATAT GAATATTATT TAACTGTAAT GTTTTAATAT CGCTGGACAT TACTAATAAA *F 4741 CCCACTATAA ACACATGTAC ATATGTATGT TTTGGCATAC AATGAGTAGT TGGGGAAAAA *F 4801 ATGTGTAAAA GCACCGTGAC CATCACAGCA TAAAGATAAC CAGCTGAAGT ATCGAATATG *F 4861 AGTAACCCCC AAATTGAATC ACATGCCGCA ACTGATAGGA CCCATGGAAG TACACTCTTC *F 4921 ATGGCGATAT ACAAGACACA CACAAGCACG AACACCCAGT TGCGGAGGAA ATTCTCCGTA *F 4981 AATGAAAACC CAATCGGCGA ACAATTCATA CCCATATATG GTAAAAGTTT TGAACGCGAC *F 5041 TTGAGAGCGG AGAGCATTGC GGCTGATAAG GTTTTAGCGC TAAGCGGGCT TTATAAAACG *F 5101 GGCTGCGGGA CCAGTTTTCA TATCACTACC GTTTGAGTTC TTGTGCTGTG TGGATACTCC *F 5161 TCCCGACACA AAGCCGCTCC ATCAGCCAGC AGTCGTCTAA TCCAGAGACC CCGGATCCGA *F 5221 TATCACTAGT TCTAGAGGTA CCGAGCTCGC GGCCGCGTTA ACCGATCCGT CGACCATGAA *F 5281 GATCAAGATC ATTGCCCCGC CAGAGCGCAA GTACTCTGTC TGGATCGGTG GCTCCATCCT *F 5341 GGCTTCGCTG TCCACCTTCC AGCAGATGTG GATCTCCAAG CAGGAGTACG ACGAGTCCGG *F 5401 CCCCTCCATT GTGCACCGCA AGTGCTTCTA AGAAGGATCG CTTGTCTGGG CAAGAGGATC *F 5461 AGGATCGGGA TGGTCTTGAT TCTGCTGGAG GAGGAGGAGG AGAAGTCGAG GAAGCAGCAG *F 5521 CGAAAGTGCA AGTGCGAGTG GTGGAAGTTT GGAGTGCAGC ACAACAAAAT CAACAACAAC *F 5581 ACCAACTACA AGATGAAAAG AGCGGAACCA CCTGCACACC ATCATCACTA TCATCATCGT *F 5641 TTTGGGCGCA TGTTGTGTGG TTCCAGCGTA TTAATATAAT TAATTTATTC CACATGAGAT *F 5701 ATGATATGAT ATACTATGTA TTTTTTGTTT TTTTTTTATT TGTAAACCTT TAATATAACA *F 5761 AGAACTACAA AAAAGTGAAA ATGAGCGAAA ATGCATATTC TGCCATTCCA CACACACACC *F 5821 AACAACACCC AACACACGCA CACCCACAAG CTTACACACA CACACATTCG CGGCATGACA *F 5881 AGGACATCAA GATAAAGAAG AACTTAAAGA AGATATTTCC CAAAGCGCAA AAAGAACACA *F 5941 CACACATTGC AAAACACAAA CAACACACTA GCGTTTTGTA CAATTCGTCA GCAACCTTAT *F 6001 GTATTATTTT TTAATTATGA TGTAATTATA AACAAAGTGA AACAAAAATA TGAAAACAAA *F 6061 AAGGAAAATC AAATCTGTCT TCTCTTTCTC CCGCTCTCCT CGCTCTCTGC TGCTAACCTC *F 6121 GCCCTCTCCT CTCTCATCTT TTTGTCTGTC TCTCTTCACA TTTTTGCCGG CCGGCAAAAT *F 6181 AATAACCCAC ACACACTCAC ACTTGGCTGC AGTTTCGCGT GCGATATTCA CACACATTCA *F 6241 AGCATACATA CATATGTATT TTTTTTTTAT TTGTACACTT TTCTAATTGC ATGCGTATCG *F 6301 ATTGATAAGT TTACGCTGAA AATGTTAATT AAAATGTGAA AATGCAACTG AAAAACTGAT *F 6361 GAAATGAAAC AACAACAAGC GAACAAGTCG ACTCTAGAGG ATCCCCGGGT ACCGAGCTCG *F 6421 AATTAATTC *F # *U FBrf0109187 *a Michelson *b A. *t 1999.8.9 *T personal communication to FlyBase *u *F *F From kcook@bio.indiana.edu Mon Aug 9 17:59:29 1999 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Mon, 09 Aug 1999 16:57:19 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: UAS constructs and insertions from Alan Michelson *F Mime-Version: 1.0 *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Subject: UAS constructs and insertions from Alan Michelson *F *F The following insertions were generated in the lab of Alan Michelson. *F Stocks containing these insertions were donated to the Stock Center July *F 22, 1999. *F *F P{w[+mc]=UAS-Egfr.B}32-26-1 Homozygous viable insertion on chromosome 3 *F P{w[+mC]=UAS-Egfr.DN.B}29-77-1 Homozygous viable insertion on chromosome 2 *F P{w[+mC]=UAS-Egfr.DN.B}29-8-1 Homozygous viable insertion on chromosome 3 *F P{w[+mC]=UAS-S.B}28-29-1 Homozygous viable insertion on chromosome 2 *F P{w[+mC]=UAS-htl.M}YYDFR-F16 Homozygous viable insertion on chromosome 3 *F P{w[+mC]=UAS-htl.DN.M}33-B40 Homozygous viable insertion on chromosome 2 *F P{w[+mC]=UAS-htl.DN.M}33-B61 Homozygous viable insertion on chromosome 3 *F P{w[+mC]=UAS-htl::lambda\cI.M}40-22-2 Homozygous viable insertion on *F chromosome 3 *F *F The following P{w[+mC]=UAS-Aos} construct and insertions have not yet been *F published: *F *F P{w[+mC]=UAS-Aos}30-102-1 Homozygous viable insertion on chromosome 1 *F P{w[+mC]=UAS-Aos}30-85-1 Homozygous viable insertion on chromosome 2 *F *F A P{w[+mC]=UAS-Aos}30-102-1; P{w[+mC]=UAS-Aos}30-85-1 stock showed a rough *F eye phenotype when crossed to an eye disk GAL4 driver. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F # *U FBrf0109188 *a Florence *b B. *t 1999.7.22 *T personal communication to FlyBase *u *F Archived. # *U FBrf0109189 *a Schaefer *b U. *c H. *d Jackle *e Y. *f He *g H. *h Bellen *i T. *j Laverty *k G. *l Rubin *t 1999.8.12 *T personal communication to FlyBase *u *F Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Subject: Goettingen lethals - set 3 *F Dated: 12 August 1999 *F *F Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of *F Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map *F the P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read *F by both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines *F is caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView. *F This communication includes revised insertion sites for some of the lines included in FBrf0108529, followed by new *F alleles and transposon insertions. *F *F Alleles with updated information (presumed to be caused by P{lacW} insertions): *F *F Original Update *F l(1)G0329[G0329] 1B7-10 ? -- multi-insert line, 1B7-10 and 9D *F l(1)G0044[G0044] 2B1-4 2A3-B2 *F l(1)G0012[G0012] 2A1-2 2B1-4 *F l(1)G0014[G0014] 2C1-2 2C1-8 *F l(1)G0025[G0025] 5D1-2 5C5-8 *F l(1)G0233[G0233] 7D3-5 7D5-9 *F l(1)G0203[G0203] 7E5-6 7E5-10 *F l(1)G0137[G0137] 8F1-2, 9A1-3 8F4-9A1 (formerly multi-insert line) *F l(1)G0126[G0126] 9F1-2 9E4-8 *F l(1)G0076[G0076] 9E7-8 9E5-8 *F l(1)G0065[G0065] 10B1-2 10A10-12 *F l(1)G0060[G0060] 11B1-4 11B14-C2 *F l(1)G0070[G0070] 17C1-2 17D *F l(1)G0147[G0147] 3A3, 18C1-2 18B (formerly multi-insert line) *F l(1)G0120[G0120] 18F1-2 18F *F l(1)G0062[G0062] 19A1-2 18F1-2 *F *F Updated transposon insertions (from multi-insert lines): *F *F Original Update *F P{lacW}G0097a 4E 4DE *F P{lacW}G0097b 9C1-2 9C *F P{lacW}G0097c 12F1-3 12F *F P{lacW}G0329a 1B7-10 (new) *F P{lacW}G0329b 9D (new) *F P{lacW}G0137a 8F1-2 eliminate *F P{lacW}G0137b 9A1-3 eliminate *F P{lacW}G0147a 3A3 eliminate *F P{lacW}G0147b 8C1-2 eliminate *F *F New Alleles (presumed to be caused by P{lacW} insertions): *F *F l(1)G0378[G0378] ? -- multi-insert line: 1B7-10, 4E1-2 *F l(1)G0389[G0389] 1C *F l(1)G0431[G0431] 2A *F l(1)G0042[G0042] 2B1-8 *F l(1)G0401[G0401] 2B1-8 *F l(1)G0406[G0406] 2B1-8 *F l(1)G0158[G0158] 2D1-2 *F l(1)G0385[G0385] semi-lethal 2E *F l(1)G0181[G0181] 3A1-4 *F l(1)G0374[G0374] 3A1-4 *F l(1)G0377[G0377] 3A1-4 *F l(1)G0445[G0445] ? -- multi-insert line: 3A1-4, 4C15-D2 *F l(1)G0152[G0152] ? -- multi-insert line: 3A1-4, 7E9-11 *F l(1)G0400[G0400] ? -- multi-insert line: 3A1-4, 8F *F l(1)G0380[G0380] ? -- multi-insert line: 3A1-4, 9E3-8 *F l(1)G0175[G0175] ? -- multi-insert line: 3A1-4, 10F *F l(1)G0434[G0434] ? -- multi-insert line: 3A1-4, 17C5-7 *F l(1)G0412[G0412] 3A3-4 *F l(1)G0375[G0375] semi-lethal ? -- multi-insert line: 3A3-4, 14B3-4 *F l(1)G0151[G0151] 3A3-6 *F l(1)G0335[G0335] 3B1-2 *F l(1)G0139[G0139] semi-lethal 3D1-4 *F l(1)G0354[G0354] 3D3-4 *F l(1)G0343[G0343] 3D5-E2 *F l(1)G0373[G0373] 3E1-4 *F l(1)G0167[G0167] semi-lethal 3E6-F2 *F l(1)G0299[G0299] semi-lethal 3F1-2 *F l(1)G0433[G0433] 3F1-2 *F l(1)G0336[G0336] 4B *F l(1)G0387[G0387] 4C5-8 *F l(1)G0273[G0273] 4C7-8 *F l(1)G0338[G0338] 4C15-D2 *F l(1)G0432[G0432] semi-lethal 4C15-D3 *F l(1)G0248[G0248] semi-lethal 4D1-2 *F l(1)G0334[G0334] 4D1-2 *F l(1)G0371[G0371] 4D1-2 *F l(1)G0420[G0420] 5C3-8 *F l(1)G0365[G0365] 5E1-2 *F l(1)G0159[G0159] 5F5-6 *F l(1)G0140[G0140] ? -- multi-insert line: 6A, 13E14-F1 *F l(1)G0149[G0149] 6B *F l(1)G0148[G0148] 6C1-2 *F l(1)G0255[G0255] 6C1-7 *F l(1)G0173[G0173] ? -- multi-insert line: 6E4-5, 13D1-2 *F l(1)G0011[G0011] semi-lethal 6F1-2 *F l(1)G0157[G0157] 6F1-2 *F l(1)G0281[G0281] 7D3-5 *F l(1)G0379[G0379] 7D20-22 *F l(1)G0356[G0356] 7E5-6 *F l(1)G0166[G0166] 7E5-10 *F l(1)G0424[G0424] 7E5-11 *F l(1)G0404[G0404] 8B1-4 *F l(1)G0415[G0415] 8B3-7 *F l(1)G0417[G0417] ? -- multi-insert line: 8B3-8, 14A *F l(1)G0397[G0397] 8D8-12 *F l(1)G0320[G0320] 8E3-11 *F l(1)G0232[G0232] 8F3-7 *F l(1)G0350[G0350] 8F6-7 *F l(1)G0230[G0230] 9B1-8 *F l(1)G0289[G0289] 9C *F l(1)G0388[G0388] 9E1-2 *F l(1)G0098[G0098] 9E1-4 *F l(1)G0351[G0351] 9E1-4 *F l(1)G0419[G0419] 9E1-4 *F l(1)G0436[G0436] 9E1-4 *F l(1)G0238[G0238] semi-lethal 9E3-4 *F l(1)G0391[G0391] 9E3-4 *F l(1)G0242[G0242] 9E3-6 *F l(1)G0247[G0247] 9E7-8 *F l(1)G0190[G0190] 10B1-2 *F l(1)G0342[G0342] 10B8-11 *F l(1)G0444[G0444] semi-lethal 10C *F l(1)G0407[G0407] semi-lethal 10E *F l(1)G0102[G0102] 10E1-4 *F l(1)G0292[G0292] 10E1-4 *F l(1)G0411[G0411] semi-lethal 10E1-4 *F l(1)G0361[G0361] 11C *F l(1)G0429[G0429] 11E1-4 *F l(1)G0182[G0182] 12A1-2 *F l(1)G0416[G0416] 12E1-2 *F l(1)G0392[G0392] 12F1-5 *F l(1)G0160[G0160] 13E1-4 *F l(1)G0437[G0437] 13E14-F2 *F l(1)G0414[G0414] 13E14-F4 *F l(1)G0438[G0438] 13F1-4 *F l(1)G0369[G0369] 13F1-11 *F l(1)G0430[G0430] 14B5-8 *F l(1)G0382[G0382] 14B7-10 *F l(1)G0006[G0006] 15A1-5 *F l(1)G0213[G0213] 15E, 15F *F l(1)G0316[G0316] 16B1-4 *F l(1)G0222[G0222] 16B6-11 *F l(1)G0358[G0358] 17C *F l(1)G0381[G0381] 18D5-8 *F l(1)G0264[G0264] 18E *F l(1)G0321[G0321] semi-lethal 18E *F l(1)G0367[G0367] 18E *F l(1)G0405[G0405] 18E *F l(1)G0441[G0441] 18E *F l(1)G0218[G0218] 18E1-2 *F l(1)G0353[G0353] 18F1-2 *F l(1)G0285[G0285] 19E *F l(1)G0393[G0393] 20AB *F *F New Transposon insertions (from multi-insert lines) *F *F P{lacW}G0378a 1B7-10 *F P{lacW}G0378b 4E1-2 *F P{lacW}G0445a 3A1-4 *F P{lacW}G0445b 4C15-D2 *F P{lacW}G0152a 3A1-4 *F P{lacW}G0152b 7E9-11 *F P{lacW}G0400a 3A1-4 *F P{lacW}G0400b 8F *F P{lacW}G0380a 3A1-4 *F P{lacW}G0380b 9E3-8 *F P{lacW}G0175a 3A1-4 *F P{lacW}G0175b 10F *F P{lacW}G0434a 3A1-4 *F P{lacW}G0434b 17C5-7 *F P{lacW}G0375a 3A3-4 *F P{lacW}G0375b 14B3-4 *F P{lacW}G0140a 6A *F P{lacW}G0140b 13E14-F1 *F P{lacW}G0173a 6E4-5 *F P{lacW}G0173b 13D1-2 *F P{lacW}G0417a 8B3-8 *F P{lacW}G0417b 14A *F P{lacW}G0213a 15E *F P{lacW}G0213b 15F *F *F # *U FBrf0109190 *a Nauber *b U. *t 1999.8.30 *T personal communication to FlyBase *u *F Personal communication to FlyBase from Ulrich Nauber, received 08/30/99. *F *F *F Concerning knirps constructs mentioned in Figure 2 of Lunde et al., 1998, *F Development 125:4145. *F *F In the legend of Figure 2 TWO constructs are mentioned: *F One is a genomic fragment tested for rescue, the other one is a kni-lacZ *F reporter construct. Both constructs and the resulting fly stocks were made *F in the lab of Herbert Jaeckle. *F *F The rescue construct consists of a 9.5 kb Sal I / Hind III fragment that *F contains about 5.5 kb of upstream sequences, the complete kni transcription *F unit (3 kb) and about 1 kb of downstream sequences. This genomic fragment *F was inserted into the Carnegie 20 vector and transformed into flies. A map *F of the construct is published in Pankratz et al., 1992 (Science 255, *F 986-989). The resulting fly stock is named P(ry+; kni-42) and is available *F from Herbert Jaeckle. *F *F The kni-lacZ reporter construct is a 4.4 kb Bam HI / Nru I upstream *F fragment of kni fused to the pCaSpeR AUG beta-gal. It is described in *F detail (with map) in Pankratz et al., 1989 (Nature 341, 337-340) and in *F Pankratz et al., 1992 (Science 255, 986-989). The fly stock is named P(w+; *F 4.4knilacZ/2-69) and is available from Herbert Jaeckle. *F *F **************************************************************** *F *F Dr. Ulrich Nauber *F Max-Planck-Institut fuer biophysikalische Chemie *F Abt. Molekulare Entwicklungsbiologie (Abt. 170) *F Am Fassberg 11 *F D-37077 Goettingen *F Tel.: ++49-551-201-1590 *F Fax: ++49-551-201-1755 *F eMail: unauber@gwdg.de *F *F **************************************************************** *F *F # *U FBrf0109191 *a Callan *b H.G. *c A. *d Klug *T personal communication to FlyBase *u *F Personal communication to FlyBase from Ulrich Nauber, received 08/30/99. *F *F *F Concerning knirps constructs mentioned in Figure 2 of Lunde et al., 1998, *F Development 125:4145. *F *F In the legend of Figure 2 TWO constructs are mentioned: *F One is a genomic fragment tested for rescue, the other one is a kni-lacZ *F reporter construct. Both constructs and the resulting fly stocks were made *F in the lab of Herbert Jaeckle. *F *F The rescue construct consists of a 9.5 kb Sal I / Hind III fragment that *F contains about 5.5 kb of upstream sequences, the complete kni transcription *F unit (3 kb) and about 1 kb of downstream sequences. This genomic fragment *F was inserted into the Carnegie 20 vector and transformed into flies. A map *F of the construct is published in Pankratz et al., 1992 (Science 255, *F 986-989). The resulting fly stock is named P(ry+; kni-42) and is available *F from Herbert Jaeckle. *F *F The kni-lacZ reporter construct is a 4.4 kb Bam HI / Nru I upstream *F fragment of kni fused to the pCaSpeR AUG beta-gal. It is described in *F detail (with map) in Pankratz et al., 1989 (Nature 341, 337-340) and in *F Pankratz et al., 1992 (Science 255, 986-989). The fly stock is named P(w+; *F 4.4knilacZ/2-69) and is available from Herbert Jaeckle. *F *F **************************************************************** *F *F Dr. Ulrich Nauber *F Max-Planck-Institut fuer biophysikalische Chemie *F Abt. Molekulare Entwicklungsbiologie (Abt. 170) *F Am Fassberg 11 *F D-37077 Goettingen *F Tel.: ++49-551-201-1590 *F Fax: ++49-551-201-1755 *F eMail: unauber@gwdg.de *F *F **************************************************************** *F *F # *U FBrf0109326 *a Bellen *b H. *t 1996.6.26 *T personal communication to FlyBase *u *F >From ag24@gen.cam.ac.uk Thu Jun 13 18:13:23 1996 *F > *F >Hi Hugo, *F > *F >. *F >. *F >. *F > *F >The difficulty is with the gene 'L30' that you suggest it hits. (I *F >gather you confirmed this in mail to Eleanor a few months back.) We *F >have no such gene, though we do have a gene RpL7 which Prosite say has *F >the 'Ribosomal protein L30 signature'. We have no cytology for that *F >gene. Is that the one whose sequence you matched (EMBL X15109) ? *F > *F >Cheers, Aubrey *F > *F > *F > *F >>From hbellen@bcm.tmc.edu Fri Jun 14 00:02:53 1996 *F > *F >> *F >>But the difficulty is with the gene 'L30' that you suggest it hits. (I *F >>gather you confirmed this in mail to Eleanor a few months back.) We *F >>have no such gene, though we do have a gene RpL7 which Prosite say has *F >>the 'Ribosomal protein L30 signature'. We have no cytology for that *F >>gene. Is that the one whose sequence you matched (EMBL X15109) ? *F > *F >I'll check again with the tech. The sequence of the cDNA is not deposited *F >and I could deposit it, I think. It's certainly L30 as it is very *F >conserved. *F > *F >Hugo *F >>From hbellen@bcm.tmc.edu Wed Jun 26 23:04:28 1996 *F > *F >Hi Aubrey, *F > *F >The story on 99/18 is complex and I don't know if we are not waisting time, *F >but for the record, here is what I found out. *F > *F >The CyO balancer carries a mutation in numb which is not associated with a *F >P-element. *F > *F >Second, the mapping data are ambiguous. Most slides show a signal at at *F >36E/F. However, one slide showed a signal at 56F. *F > *F >Third, the gene that we isolated is very homologous to L30 and is certainly *F >the true L30 protein of Drosophila. *F > *F >Fourth, the L30 maps to 36E/F. *F > *F . *F . *F . *F > *F >Hugo # *U FBrf0109360 *a Bloomington Drosophila Stock Center *b ?. *t 1999.7.19 *T personal communication to FlyBase *u *F From matthewk@fly.bio.indiana.edu Mon Jul 19 14:51:17 1999 *F To: curators@morgan.harvard.edu *F Subject: new TM6C balancer for curation *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Pascal Heitzler, IGBMC, Strasbourg, France *F To: Bloomington Drosophila Stock Center *F Dated: 19 July 1999 *F Background: Bloomington has a stock with this new version of TM6C, *F which is not in FlyBase. Balancer short genotype: TM6C, ryCB Sb1 Tb1. *F Information communicated: *F TM6C, ryCB Sb1 Tb1 was made from the extant TM6C and TM6B: *F ryCB from TM6B, ryCB was crossed onto the viable TM6C balancer, and then *F Sb1 and Tb1 but not cu1 from the TM6C, cu1 Sb1 Tb1 were added. # *U FBrf0109361 *a Bloomington Drosophila Stock Center *b ?. *t 1999.8.10 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: TM3 variant *F Date: 10 August 1999 *F *F Information communicated: *F *F A TM3 balancer in a Bloomington stock has been shown by Helen Francis-Lang, Exelixis Pharmaceuticals Inc., to carry a ca allele. The origin of this allele is unknown. The short genotype for the new balancer is: TM3, Sb[1] Ser[1] ca[*] *F # *U FBrf0109396 *a Bray *b S. *t 1999.7.30 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Jul 29 17:47:34 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 29 Jul 1999 17:47:34 +0100 *F To: sjb32@mole.bio.cam.ac.uk *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 29 Jul 1999 17:47:59 +0100 *F Content-Length: 1904 *F Dear Sarah, *F I am curating your paper for FlyBase: *F Jennings et al., 1999, Molec. Cell. Biol. 19(7): 4600--4610 *F In this you use a 'sal-Gal4' line which you reference to 2 papers: *F de Celis et al., 1997, Development 124(10): 1919--1928 *F Thomas et al., 1995, Genetics 139(1): 203--213 *F We do not have a record for a sal-Gal4 line in either of these papers, *F but both papers use the '459.2' Gal4 line. *F Is the 459.2 line actually an enhancer trap in a spalt gene which you *F have used in the Molec. Cell Biol. paper ? If so, do you know whether *F the insertion causes a mutation in spalt or is it just reflecting the *F expression of the spalt gene without causing a mutant phenotype ? *F Also, which spalt gene is it inserted in - spalt major, spalt adjacent *F or spalt related ? *F If the sal-Gal4 line you used in the Molec. Cell. Biol. paper is not *F 459.2, I would be grateful if you could tell me some details about the *F line. Is it an enhancer trap line or a promoter fusion in which a *F spalt promoter drives Gal4. If it is an enhancer trap, is it a P{GawB} *F insertion ? If it is a promoter fusion, which vector is it in e.g. *F CaSpeR, Carnegie 20 (this helps to work out the marker gene - w or ry *F in the construct). Again which spalt gene is it - spalt major, spalt *F adjacent or spalt related ? *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From sjb32@mole.bio.cam.ac.uk Fri Jul 30 14:36:23 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 30 Jul 1999 14:36:23 +0100 *F X-Sender: sjb32@131.111.36.9 *F Mime-Version: 1.0 *F Date: Fri, 30 Jul 1999 14:34:23 +0000 *F To: Gillian Millburn (Genetics) *F From: Sarah Bray *F Subject: Re: FlyBase query *F Dear Gillian, *F Re your query: *F > *F >Jennings et al., 1999, Molec. Cell. Biol. 19(7): 4600--4610 *F > *F >In this you use a 'sal-Gal4' line which you reference to 2 papers: *F > *F >de Celis et al., 1997, Development 124(10): 1919--1928 *F >Thomas et al., 1995, Genetics 139(1): 203--213 *F > *F >We do not have a record for a sal-Gal4 line in either of these papers, *F >but both papers use the '459.2' Gal4 line. *F > *F >Is the 459.2 line actually an enhancer trap in a spalt gene which you *F >have used in the Molec. Cell Biol. paper ? *F Yes. *F Insertion is homozygous viable: no phenotype observed. *F >Also, which spalt gene is it inserted in - spalt major, spalt adjacent *F >or spalt related ? *F No idea I'm afraid. Pattern looks like spalt major as far as I have seen, *F but we haven't really examined it in sufficient detail to be able to *F distinguish. As far as I know it isn't mapped moleclarly. *F bye for now, *F Sarah. *F Sarah Bray *F Dept. of Anatomy, *F Univ. of Cambridge, *F Downing Street. *F Cambridge, CB2 3DY *F England. *F Tel:44-1223-333792(office)/ 333751(lab) *F fax:44-1223-333786 *F From gm119@gen.cam.ac.uk Fri Jul 30 14:39:44 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 30 Jul 1999 14:39:44 +0100 *F To: sjb32@mole.bio.cam.ac.uk *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 30 Jul 1999 14:40:11 +0100 *F Content-Length: 449 *F Dear Sarah, *F thanks thats great ! I'll make the information that 459.2 is a sal-Gal4 *F line a personal communication from you to FlyBase. *F I'll put it under spalt major for now, *F Gillian # *U FBrf0109616 *a Doerig *b R. *t 1999.8.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Aug 10 02:09:16 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: P lethals from Paul Lasko *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: P lethals from Paul Lasko *F The following information was communicated by Ruth Doerig in Paul Lasko's *F lab, McGill University 7/99. *F 1. The P{w+mC=lacW}k09017a insertion (FBti0006603) at 39E1-4 is *F associated with a lethal mutation based on its noncomplementation with *F Df(2L)TW65 and Df(2L)TW161 and its complementation with Df(2L)C', *F Df(2L)DS5, Df(2L)DS6, Df(2L)DS8, Df(2L)TW84 and Df(2L)Acon-21. *F The P allele should probably be named P{w+mC=lacW}l(2)k09017ak09017a *F 2. The P{ry+t7.2=PZ}02660b insertion is associated with a lethal *F mutation based on its noncomplementation with Df(2L)OD15, Df(2L)TW3, *F Df(2L)TW50 and Df(2L)TW137. (This information is also available from the *F BDGP web site.) The l(2)02660r entry (FBgn0025775) refers to this P *F insertion. Lasko et al. refer to the lethal locus defined by the *F P{ry+t7.2=PZ}02660b insertion as l(2)37Ad. *F The P allele should probably be named P{ry+t7.2=PZ}l(2)37Ad02660b. # *U FBrf0109716 *a Flores *b C. *t 1999.7.25 *T personal communication to FlyBase *u *F >From ccflores@facstaff.wisc.edu Sun Jul 25 05:29:05 1999 *F To: Michael Ashburner *F Subject: RE: help FlyBase please *F Bill and Michael Ashburner, *F I found the microsatellite at 35F simply by searching the database for *F simple repeats. I did a search just now with the sequence from this area *F and found that the repeat lies in the 2nd intron of the crp (cropped) gene, *F A.K.A. l(2)35Fd. The region I amplified roughly corresponds to bases 66390 *F to 66490 of P1 clone DS02740. Berkeley's 'clone curator' made it easy to *F trace this down to the gene and region within the gene. This puts it very *F close to the insertion EP(2)0721. *F Sincerely, *F Carlos Flores *F >From ma11@gen.cam.ac.uk Sat Jul 24 18:13:17 1999 *F To: wrengels@facstaff.wisc.edu *F Subject: help FlyBase please *F Bill *F In Flores & Engels PNAS 96:2964 *F What is the gene corresponding to '35F' locus of a microsatellite ? *F Thanks *F Michael # *U FBrf0109729 *a Francis-Lang *b H. *t 1999.8.10 *T personal communication to FlyBase *u *F Personal communication from: Helen Francis-Lang, Exelixis Pharmaceuticals Inc. *F To: Bloomington Drosophila Stock Center *F Subject: sqd[j6E3], l(3)j6E3[j6E3] and EP(3)3631 *F Dated: 10 August 1999 *F *F Information communicated: *F *F sqd[j6E3] is fully viable over both Df(3R)urd (87F9-10;88B1-2) and Df(3R)l26c (87E1-2;87F11-12). Fertility of Df/sqd[j6E3] adults was not tested. Therefore, the chromosome known as l(3)j6E3 includes both P{lacW}sqd[j6E3] and l(3)j6E3[j6E3]. *F *F P{EP}EP(3)3631 is fully viable over Df(3R)urd but lethal over Df(3R)l26c. Fertility of Df(3R)urd/P{EP}EP(3)3631 adults was not tested. *F # *U FBrf0109761 *a Gelbart *b W.M. *t 1999.7.20 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Tue Jul 20 03:04:46 1999 *F To: rd120@gen.cam.ac.uk *F Subject: Re: BDGP lines *F Hi Rachel, *F Please curate this as a pc from me. *F ============================================================= *F Line(#) BDGP Cytology Final Conclusion *F \---------- --------------- ---------------------------------------------- *F l(2)k09624 22B1-22B2 No P phenotype *F l(2)k07918 22B6-22B7 No P phenotype *F l(2)k09932 22C1-22C2 No P phenotype *F l(2)03953 22D1-22D2 No P phenotype *F l(2)k11038 22D1-22D2 No P phenotype *F l(2)09639 22D1-22D2 No P phenotype *F l(2)s4989 22D3-22D4 No P phenotype *F l(2)00231 22E2-22E3 No P phenotype *F l(2)02321 22F3-22F4 No P phenotype *F l(2)k11021 22B1-22B2 No P phenotype *F l(2)k09903 22D1-22D2 No P phenotype *F l(2)k11704 22A3-22A4 OK - P lethality consistent with deletion data *F l(2)k08232 22E1-22E2 OK - P lethality consistent with deletion data *F l(2)10638 22F1-22F2 OK - P lethality and dpp allelism consistent *F with deletion data *F l(2)04111* 22A4-22A6 OK - P lethality consistent with deletion data *F \*NOTE: only the l(2)04111k13009 line was tested. *F \----- End Included Message ----- # *U FBrf0109775 *a Gibson *b G. *t 1999.7.26 *T personal communication to FlyBase *u *F >From ma11@gen.cam.ac.uk Sat Jul 24 19:02:27 1999 *F To: ggibson@unity.ncsu.edu *F Subject: Help FlyBase please *F Greg *F So FB can properly curate yr Gibson et al., 1999, Genetics 151(3): 1081--1091 *F can you tell me was Amy.2.6 from Amy-d or Amy-p ? *F Thanks *F Michael *F >From ggibson@unity.ncsu.edu Mon Jul 26 14:45:41 1999 *F To: 'Michael Ashburner (Genetics)' *F Subject: amy-p nor amy-d *F Michael, *F The short answer, regrettably, is neither. The microsat. we list as 2.6 was *F initially reported in Schlotterer, Vogl and Tautz (1997: Genetics *F 146:309-320) as a subclone derived from DS00062, which maps to 54AB in the *F Amy region. When I got your message on Saturday, I ran a BLAST search *F against FB, and the sequence actually maps to the complete sequence of *F DS00058, which has been localized to 31AB according to FB. *F So then I went back to my recombination map, which was assembled by *F MapMaker, but based on individual pairwise distances, marker 2.6 could lie *F close to marker 2.3 at 35B. There isn't a lot of recombination near the *F centromere, but there were several double recombinants in the data set. *F Since there were no significant QTLs in the region, the conclusions of the *F paper aren't altered, but I will send a corrigendum off to Genetics once I *F hear back from Christian Schlotterer. *F On a similar matter, we have just discovered that an ASO marker against a *F SNP in the Dopamine receptor that is supposedly localized at 35EF is *F genetically at cytological interval 88 or 89 (this time there is no *F ambiguity in the map - the recombination frequencies are perfectly in *F agreement with published data). There is no picture of the in situ in the *F original paper, and when you do a BLAST search, there is no match of DopR to *F a P1 even though 35EF is shown as almost finished sequence. *F Roger Hoskins and I found a few inconsistencies between mapped positions of *F STSs and ESTs as reported on FB while assembling our SNP map of 10 strains - *F which should be submitted in a few weeks. After 10 years in the business I *F should be more wary of published data, but given the volume of data I guess *F this is to be expected. Fly Base is a truly great resource, so thanks for *F all of your care and effort. *F Greg # *U FBrf0109798 *a Goodman *b C.S. *t 1999.8.19 *T personal communication to FlyBase *u *F >From goodman@uclink4.berkeley.edu Thu Aug 19 01:57:12 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 19 Aug 1999 01:57:12 +0100 *F Mime-Version: 1.0 *F Date: Wed, 18 Aug 1999 18:01:59 -0700 *F To: Michael Ashburner (Genetics) *F From: 'Corey S. Goodman' *F Cc: denise_montell@qmail.bs.jhu.edu *F Michael, *F Denise Montell and Meg Winberg in my lab have conferred, and we would like *F to formally change the name of Dtrk to 'off-track' with a designation *F 'otk'. *F Thanks very much *F Best wishes *F Corey *F Prof. Corey S. Goodman *F Howard Hughes Medical Institute *F Department of Molecular and Cell Biology *F 519 LSA *F University of California, Berkeley *F Berkeley, CA 94720 USA *F phone: 510 643-9949 *F office phone: 510 643-9948 *F lab phone: 510 642-9084 *F FAX: 510 643-5548 *F e-mail: goodman@uclink4.berkeley.edu # *U FBrf0109890 *a Heitzler *b P. *t 1999.7.20 *T personal communication to FlyBase *u *F From cy200@gen.cam.ac.uk Wed Jul 14 09:56:54 1999 *F To: pascal@igbmc.u-strasbg.fr *F Subject: FlyBase Query (W88) *F Dear Dr Heitzler, *F I am curating a paper for FlyBase: *F Franc et al. Science, 1999 284(5422) 1991--1994. *F I have been correspondence with Nathalie Franc about this paper. In *F response to the question below, she suggested I get in touch with *F you, since you had generated the W88 line. *F 1.Df(2L)TE99(Z)XW88 *F In your paper you use a Deficiency which you call W88. We have no *F record of this aberration and you did not describe its generation. *F Could you tell me where this aberration came from? *F If this line has been used in another paper, it would be useful to know *F which one and what name was used. If not, then any genetic information *F about this line would be good, mutagen, breakpoints etc. *F Any information that you give will be entered into FlyBase as a *F personal communication from you. *F Thank you for your help, *F Chihiro *F From pascal@titus.u-strasbg.fr Tue Jul 20 19:38:14 1999 *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (W88) *F Dear Dr Chihiro Yamada *F Thank you very much for your message, I will try to explain this *F complicated XW88 story. The TE99(Z) natural w+ transposon from Gunnar *F Ising is inserted in the 21C region. *F I found that this transposon is inserted within the 5' end regulatory *F region of the ushaped gene and is associated with a hypomorphic viable *F ush phenotype (Cubbada et al 1997 *F ). *F I performed X-ray w- derivatives from TE99(Z) (unpublished results). *F Among numerous putatives, TE99(Z)XW88 was quite unusual because it *F exhibits a new dominant phenotype due to ectopic ush expression *F (unpublished observations). The TE99(Z)XW88 chromosome is associated *F with the small deletion published in our Science paper. This chromosome is not ush- because Df(2L)TE99(Z)XW88/ush- flies show *F the viable ush loss of function phenotype like for the original *F TE99(Z)/TE99(Z) or TE99(Z)/ush- combinations. The best explanation for *F the new dominant phenotype is that in Df(2L)TE99(Z)XW88, the ush *F regulatory region juxtapose enhancer sequences of the ex gen e. *F I induced revertants of the dominant phenotype of Df(2L)TE99(Z)XW88. I *F found three revertants which as expected reduce further or abolish the *F ushaped function; of course they retained also the original deletion. Their *F characteristics are as following: *F \- In(2L)TE99(Z)XW88-DV1, *F X-ray induced, associated to an inversion 21C;23A, stronger viable ush *F allele as the original chromosome. *F \- TE99(Z)XW88-DV2, *F EMS-induced, ush- chromosome *F \- TE99(Z)XW88-DV3, *F EMS-induced, ush- chromosome *F I hope you have understand my XW88 story, please note that the deletion use *F in our Science paper is in fact the chromosome that carries the EMS-induced *F ushXW88-DV2 allele. Thank you for your attention. *F Yours sincerely, *F Pascal Heitzler. # *U FBrf0109912 *a Hewett-Emmett *b D. *t 1999.8.12 *T personal communication to FlyBase *u *F >From cdna-owner@fruitfly.bdgp.berkeley.edu Thu Aug 12 18:16:15 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 12 Aug 1999 18:16:15 +0100 *F X-Authentication-Warning: fruitfly.bdgp.berkeley.edu: majordom set sender to owner-cDNA@fruitfly.berkeley.edu using *-f *F Date: Thu, 12 Aug 1999 12:15:56 -0500 *F From: David Hewett-Emmett *F Subject: cdna AF160881 *F To: ''cdna@fruitfly.berkeley.edu'' *F MIME-version: 1.0 *F Content-Transfer-Encoding: 8bit *F X-MIME-Autoconverted: from quoted-printable to 8bit by fruitfly.bdgp.berkeley.edu id KAA04623 *F Sender: owner-cDNA@fruitfly.berkeley.edu *F I was very interested by the new complete D.melanogaster cds. AF160881 that you deposited in GenBank on 8/3/99. *F My interest is that it includes the coding region of a complete alpha-carbonic anhydrase (bp 287-1099 encode a 270 aa isozyme), which on the basis of analyzing a P1 genomic sequence (L39622) in 1995, we named CAH1 (Hewett-Emmett and Tashian 1996). At that time, we could not from the s *F There is however one problem with your cDNA clone and its current annotation. The translated sequence you describe (1420-2628) is from a transposon Tn10 (cf. GenBank entry AP000342, which gives the almost identical plasmid R100 sequence) which interrupts the 3' untranslated region of *F This is written because of my interest in the CAH1 gene and in seeing the annotations of it in the databases are as helpful to other users as possible. *F REF: D. Hewett-Emmett and R.E. Tashian (1996) Functional diversity, conservation, and convergence in the evolution of the alpha-, beta-, and gamma-carbonic anhydrase gene families. Mol. Phyl. Evol. 5: 50-77. *F In admiration for the wealth of Drosophila genome project data that is being made available and with best wishes *F David Hewett-Emmett Ph.D. *F Human Genetics Center, *F P.O. Box 20334, *F U.Texas - Houston, *F Houston, TX 77225-0334. *F TEL: 713-500-9835 *F FAX: 713-500-0900 *F email: davidhe@utsph.sph.uth.tmc.edu *F From: Michael AshburnerSMTP:ma11@gen.cam.ac.uk *F Sent: Thursday, August 12, 1999 12:40 PM *F To: cdna@fruitfly.bdgp.berkeley.edu; davidhe@utsph.sph.uth.tmc.edu *F Subject: Re: cdna AF160881 *F David - this CA is included in the Adh region that has been fully annotated *F and is discussed in a paper in press in GENETICS (Sept 99 issue). Indeed *F I returned the proofs just 4 hours ago. You can see the paper on *F the www.fruitfly.org web site as a PDF file. We call the gene BG:DS00941.1 *F in this paper but say that it encodes a CA. Here is the translation we used: *F >BG:DS00941.1 270 AAs *F MSHHWGYTEENGPAHWAKEYPQASGHRQSPVDITPSSAKKGSELNVAPLK *F WKYVPEHTKSLVNPGYCWRVDVNGADSELTGGPLGDQIFKLEQFHCHWGC *F TDSKGSEHTVDGVSYSGELHLVHWNTTKYKSFGEAAAAPDGLAVLGVFLK *F AGNHHAELDKVTSLLQFVLHKGDRVTLPQGCDPGQLLPDVHTYWTYEGSL *F TTPPCSESVIWIVFKTPIEVSDDQLNAMRNLNAYDVKEECPCNEFNGKVI *F NNFRPPLPLGKRELREIGGH *F I have just checked this with BLASTP at thee NCBI and it looks *F very convincing ! *F I cannot answer any other questions raised in yr email to Berkeley, but *F I am sure that they will be back to you. *F I missed your paper in MPE, indeed it seems not to be in FlyBase. *F Would it be possible for you to fax me a copy to +44 1223 333992 ? *F I will _try_ (but cannot promise) to add a note in proof to our paper. *F Michael Ashburner *F Michael Ashburner, Phone: +44 1223 333969 *F Department of Genetics, Fax: +44 1223 333992 *F Downing Streeet, e-mail: m.ashburner@gen.cam.ac.uk *F Cambridge, CB2 3EH, England. *F >From davidhe@utsph.sph.uth.tmc.edu Thu Aug 12 22:31:11 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 12 Aug 1999 22:31:11 +0100 *F Date: Thu, 12 Aug 1999 16:31:10 -0500 *F From: David Hewett-Emmett *F Subject: cdna AF160881 *F To: 'Michael Ashburner' *F MIME-version: 1.0 *F Content-transfer-encoding: quoted-printable *F Content-Type: text/plain; charset='us-ascii' *F Content-Length: 5840 *F Michael: *F Thanks for such a quick response. The 270 aa protein looks to be *F identical to what I see. I will FAX the paper this afternoon. Also, I *F will look at the website. *F I do need to make a relatively minor correction to one statement in my *F email. In the 1996 paper we named the Drosophila gene CAH as you will *F see. In the interim, I found another distinct Drosophila CAH -- a *F fragment coded by an EST (AA246259). About a year ago, I wrote a *F lengthy review for a book which will likely come out late this year or *F in 2000. In this later review, I used the nomenclature CAH1 and CAH2. *F I have just looked at AA246259 again and it has re-kindled my interest *F in CAH2! There are now several overlapping ESTs (e.g. AI109097, *F AI260114) giving a protein sequence that looks complete -- unlike CAH1, *F there appear to be no genomic sequence corresponding to them. *F Best Wishes *F David Hewett-Emmett *F From gm119@gen.cam.ac.uk Fri Aug 20 12:53:18 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 20 Aug 1999 12:53:18 +0100 *F To: davidhe@utsph.sph.uth.tmc.edu *F Subject: CAH1 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 20 Aug 1999 12:54:12 +0100 *F Content-Length: 1720 *F Dear Dr. Hewett-Emmett, *F Michael passed on your correspondence about CAH1 and also your paper *F 'Hewett-Emmett, Tashian, 1996 Molec. Phylog. Evol. 5: 50--77' for me to *F curate for FlyBase. *F I will curate the information that you wish the gene to be called CAH1 *F rather than CAH as you called it originally as a personal communication *F from you to FlyBase. *F Would it be OK for me to include the following information about CAH2 *F from your e-mail in the personal communication: *F >In the interim, I found another distinct Drosophila CAH -- a fragment *F >coded by an EST (AA246259). About a year ago, I wrote a lengthy review *F >for a book which will likely come out late this year or in 2000. In *F >this later review, I used the nomenclature CAH1 and CAH2. *F >I have just looked at AA246259 again and it has re-kindled my interest *F >in CAH2! There are now several overlapping ESTs (e.g. AI109097, *F >AI260114) giving a protein sequence that looks complete -- unlike CAH1, *F >there appear to be no genomic sequence corresponding to them. *F I would create a new gene called 'CAH2' and it would have the *F information that it corresponds to the EST's AA246259, AI109097 and *F AI260114 under it. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From davidhe@utsph.sph.uth.tmc.edu Fri Aug 20 18:01:53 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 20 Aug 1999 18:01:53 +0100 *F Date: Fri, 20 Aug 1999 12:02:06 -0500 *F From: David Hewett-Emmett *F Subject: RE: CAH1/CAH2 *F To: 'Gillian Millburn' *F MIME-version: 1.0 *F Content-transfer-encoding: 7bit *F That's fine to release it now. *F If you give me a day or so, I can give you the complete list of ESTs for CAH2 but I need to check them out individually. *F David *F From davidhe@utsph.sph.uth.tmc.edu Sat Aug 21 06:45:18 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 21 Aug 1999 06:45:18 +0100 *F Date: Sat, 21 Aug 1999 00:43:02 -0500 *F From: David Hewett-Emmett *F Subject: RE: CAH2 *F To: 'Gillian Millburn' *F MIME-version: 1.0 *F Content-transfer-encoding: quoted-printable *F Content-Type: text/plain; charset='us-ascii' *F Content-Length: 1972 *F Gillian: *F I have checked the ESTs. The first batch of 10 are almost identical (they vary a bit in length and where they start and finish). The central region and 3' end have only a single representative each. *F 5' end (encodes N-terminal region): *F AA246259, AI297502, AI404891 (longest EST), AI257332, AI113987, AA263558, AI517161, AA942429, AI109423, AI388709. *F Central: *F AI109097 *F 3' end (Encodes C-terminal region): *F AI26011 *F The encoded protein is probably 335 aa: *F MRRCRNTPFAIVIAPILICASLVLAQDFGYEGRHGPEHWSEDYARCSGKH 50 *F QSPINIDQVSAVEKKFPKLEFFNFKVVPDNLQMTNNGHTVLVKMSYNEDE 100 *F IPSVRGGPLAEKTPLGYQFEQFHFHWGENDTIGSEDLINNRAYPAELHVV 150 *F LRNLEYPDFASALDKDHGIAVMAFFFQVGDKSTGGYEGFTNLLSQIDRKG 200 *F KSVNMTNPLPLGEYISKSVESYFSYTGSLTTPPCSEEVTWIDFTTPIDIT 250 *F EKQLNAFRLLTANDDHLKNNFRPIQPLNDRTLYKNYIEIPIHNMGSIPLV 300 *F DAENAAGKWRAQAAAVLLPLVVLAALSRTSIFRGF* 335 *F NOTES: *F (1) Most active-site residues conserved in the active alpha- CA enzymes are conserved in this sequence. Exception *F is \#136 Asp (His in all active carbonic anhydrases except *F the prokaryotes Erwinia and Klebsiella which have Asn). *F (2) At residue \#233 (shown as 'P') which is also a conserved site (Pro) in active alpha-CAs: *F AI260114 encodes Pro (bp 229-231 of DNA sequence) *F AI109097 encodes Ser (late in the DNA sequence) *F (3) At residue \#177bp (shown as 'Q'), *F AI260114 encodes a 'stop' (bp 32-34 of the DNA sequence). *F AI109097 bp.408-410 encodes Gln. *F Examination of bp 1-34 of AI260114 shows no similarity *F with bp 377-410 of AI109097. *F Sequencing of additional ESTs and/or genomic sequence will eventually resolve whether the alternatives chosen in NOTES (2) and (3) are correct. *F David Hewett-Emmett Ph.D. *F Human Genetics Center, *F University of Texas-Houston HSC, *F P.O. Box 20334, *F Houston, TX 77225-0334, USA *F TEL: 713-500-9835 *F FAX: 713-500-0900 *F email: davidhe@utsph.sph.uth.tmc.edu # *U FBrf0110054 *a Kavaler *b J. *t 1999.8.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Jul 23 12:11:51 1999 *F To: jkavaler@email.vill.edu *F Subject: Helping FlyBase *F Dear Joshua, *F We have heard from Elspeth Bruford (HUGO Nomenclature Committee) that *F the gene symbol for Past-1 corresponds to 'Putative Achaete Scute *F Target 1'. We would like to capture this information for the FlyBase *F gene record. Is it OK if I cite you as the source for this *F information? *F With best wishes, *F Rachel. *F From jkavaler@email.vill.edu Mon Aug 02 18:24:54 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase *F Dear Rachel, *F Yes, you may cite me for this information. *F Josh # *U FBrf0110101 *a Kovalick *b G. *t 1999.7.28 *T personal communication to FlyBase *u *F From kovalick@msmail.muohio.edu Wed Jul 28 01:16:37 1999 *F To: 'Chihiro Yamada' *F Subject: RE: FlyBase Query *F The B118 deletion I referred to in my conference abstract is associated with *F the T(1;Y)B118 translocation. Kevin Mitchell, formerly of Corey Goodman's *F lab, had been doing some physical mapping in the region of the NetA and NetB *F genes, which are located in the 12E-F region. As part of his studies, he had *F mapped the approximate breakpoints of some X;Y translocations with previously *F reported breakpoints in the 12E-13A region. He indicated to me that *F T(1;Y)B118 had a breakpoint on the X chromosome between NetB and rut. The two *F genes I study are Agr and Agr2, both of which are located in 12F between NetB *F and rut. I was interested in positioning the breakpoint of this translocation *F in relation to Agr and Agr2. Many males carrying the translocation are both *F viable and fertile (actually about 50-60% seem to die during the larval *F stage), so as a first experiment, I simply extracted genomic DNA from these *F males and did a Southern, using labeled Agr and Agr2 cDNAs. To my surprise, *F the 3' half of Agr and all of Agr2 appears to be missing. I have since *F repeated this experiment several times, with identical results. I have tried *F to determine the extent of this deletion: One breakpoint occurs within the *F Agr gene; the other appears to be 20-25 kb away. I don't believe any of the *F rut gene is deleted, which is consistent with the literature. I also don't *F believe that any of the NetB coding region is deleted. I haven't precisely *F mapped the location of NetB or rut in relation to Agr or Agr2, so this *F information is really an educated guess. I hope to gather some more precise *F information once I've re-established my lab in Texas. If you need any *F additional information, please let me know. I can be reached at this email *F address until August 4; after August 15, I can be reached at *F kovalick_g@utpb.edu. I hope this information clarifies things for you. *F \----- Begin Included Message ----- *F Dear Dr Kovalick, *F I am curating your abstract for FlyBase: *F Kovalick and Ray, Society for developmental Biology, 58 1999 *F (as published in Developmental Biology 210(1) 209 1999) *F You mention a chromosomal aberration B118. We do have in our records *F a B118 translocation, T(1;Y)B118: *F \*a T(1;Y)B118 *F \*z FBab0006664 *F \*B 12F1;h1-h17 *F \*C Translocation *F \*c All limits from polytene analysis (citation unavailable) *F \*G l(1)dd4 << bk1 << NetA << rut << bk2 *F \*w Merriam and colleagues. *F \*o X ray *F \*O Dp(1;Y)BSYy+ *F \*p male viable *F \*x FBrf0066905 == Lindsley and Zimm, 1992, book *F \*x FBrf0102049 == Merriam et al., 1998.4.24, personal communication *F However your abstract seems to suggest that your 'B118' is new and *F distinct from this one. Is your 'B118' a straightforward deficiency? *F If so, is it about to be published anywhere? If not please could *F you send me a bit more information (discoverer, mutagen, breakpoints *F ...). This information is very useful to us as it impinges on the map *F data for the 'Agr2' gene. # *U FBrf0110154 *a Laverty *b T. *t 1998.6.4 *T personal communication to FlyBase *u *F Date: Thu, 4 Jun 1998 12:25:39 -0700 (PDT) *F From: Sima Misra *F To: Amy Beaton *F cc: Todd Laverty , *F Aubrey de Grey , flybase-update@morgan.harvard.edu, *F Sima Misra *F Subject: Re: Fwd: Confusion concerning l(2)04524 *F . *F . *F > >Date: Tue, 2 Jun 1998 11:12:44 -0700 *F > >To: abeaton@uclink4.berkeley.edu *F > >From: tlaverty@uclink4.berkeley.edu (Todd Laverty) *F > >Subject: Re: Fwd: Confusion concerning l(2)04524 *F > > *F > >Dear Ed, *F > >I remade the slide for l(2)04524 and it appears to me that it maps to *F > >42D1-2. This is consistent with what I thought after reanalyzing the *F > >original slide although it was of poor quality. *F . *F . *F > >Todd *F > > *F > >Todd Laverty *F > >Rubin Lab # *U FBrf0110360 *a Min *b K.T. *t 1999.7.28 *T personal communication to FlyBase *u *F From: Kyung-Tai Min *F To: c.yamada@gen.cam.ac.uk *F Subject: bubblegum information *F >3.Map position of bgm *F >On p1985 you write 'The genomic sequence corresponded to that located *F >at position 34F1-2...'. How did you establish this? Did you identify *F >an EST or P1 clone with the bgm sequence? If so it would be useful tous *F if we could link the appropriate EST or clone to bgm. Could you *F >give me the name or identifier of the EST/clone that you found so that *F >I can do this? *F The number of the EST clone is LD10778. # *U FBrf0110667 *a Rodriguez *b A. *t 1999.7.30 *T personal communication to FlyBase *u *F From rodrig02@utsw.swmed.edu Fri Jul 30 22:42:27 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 30 Jul 1999 22:42:27 +0100 *F Date: Fri, 30 Jul 1999 16:42:36 -0500 *F From: Antony Rodriguez *F Subject: gene update *F To: 'Gillian Millburn (Genetics)' *F MIME-version: 1.0 *F X-Mailer: Mozilla 4.61 (Macintosh; I; PPC) *F Content-Type: multipart/mixed; boundary='------------6EB8C1F9C8AEF7749A62F847' *F Content-transfer-encoding: 7BIT *F X-Accept-Language: en *F Content-Length: 3458 *F Dear Dr. Millburn, *F I just wanted to let you know that our paper has been published as follows *F so that you could curate our information, and most of the inforamtion that *F I sent you several weeks ago is in the paper below: *F Rodriquez,A., Oliver,H., Zou,H., Chen,P., Wang,X. and Abrams,J.M. Dark, a *F Drosophila Homolog of Apaf-1/Ced-4, functions in an Evolutionarily *F Conserved Death Pathway, Nature Cell Biology 1(5): 272-279 (1999). *F The only thing that is not mentioned in the paper is that the dark gene *F corresponds to 'anon-53Fa' in the 53F1-F2 genomic region. Thanks in *F advance and if you need more information just ask. # *U FBrf0110673 *a Roote *b J. *t 1999.7.7 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Wed Jul 07 11:09:18 1999 *F To: Michael Ashburner *F Subject: k08106 *F The k08106 story: *F 2 inserts, 35F4-5 and 35F11-12. *F Data from excision screen: *F 18 excisions, 14 lethal, 4 viable (over RA5). Of the 14 lethals 11 *F have very pale eye colour, weak crp phenotype and lethal over RA5. 3 are *F precise deletions from crp to 35Fg; 2 of which are w-, 1 is wvar. *F This suggests: *F 1) the 35F4-5 insert is a v weak crp allele (narrow, pointed wings) *F and has a weak w'+' phenotype. *F 2) the 35F11-12 insert is the l(2)35Fg allele. *F 3) l(2)35Fg is proximal of chif (all 3 deletions are chif-) *F 2nd message: *F We have 14 derivatives of k08106, 11 of which have a very pale eye colour, *F 1 is variegating and 2 are w-. *F The bit you need to know is this: *F The 2 white-eyed stocks are deletions extending from crp to l(2)35Fg. *F They partially complement crp; *F do not complement l(2)35Fb, 35Fc, 35Ff, cni, fzy, cact, 35Fe, chif, 35Fg; *F complement dac. *F They are called Df(2L)k08106-rv6 and rv15. *F John # *U FBrf0110741 *a Schloetterer *b C. *t 1999.8.20 *T personal communication to FlyBase *u *F >From christian.schloetterer@vu-wien.ac.at Fri Aug 20 08:30:41 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 20 Aug 1999 08:30:41 +0100 *F X-Sender: schlotc@i122server.vu-wien.ac.at *F Mime-Version: 1.0 *F Date: Fri, 20 Aug 1999 09:33:37 +0200 *F To: Michael Ashburner (Genetics) *F From: Christian Schloetterer *F Subject: Re: Drosophila Microsatellites *F Dear Michael, *F many thanks for your comments on our microsatellite list. I have gone *F through the comments concerning our web page. Below you find my *F explanations/comments. *F If you have further questions, please do not hesitate to ask! *F Best, *F Christian *F date_of_fb_update: 990726 *F \# *F microsatellite_symbol: DM73.alt ; reference: Vienna.990724.curated *F forward_primer_sequence: accaaagtgaaaacacaacaag ; reference: *F Vienna.990724.curated *F reverse_primer_sequence: acgctgctcccctcacacacac ; reference: *F Vienna.990724.curated *F temperature: 54 ; reference: Vienna.990724.curated *F . *F gene: MS:DM73.alt ; reference: FlyBase *F gene_comment: Same as MS:DM73 ?? *F pcr_product_size: 88-166 ; reference: Vienna.990724.curated *F number_of_alleles: 32 ; reference: Vienna.990724.curated *F observed_heterozygosity: 0.95 ; reference: Vienna.990724.curated *F variance: 52.56 ; reference: Vienna.990724.curated *F . *F IT IS THE SAME LOCUS, BUT ONE PRIMER SET AMPLIFIES TWO ADJACENT *F MICROSATELLITES (DM73 ALT), WHILE THE OTHER ONE AMPLIES ONLY A SINGLE *F MICROSATELLITE. *F date_of_fb_update: 990726 *F \# *F microsatellite_symbol: Antp1 ; reference: Vienna.990724.curated *F microsatellite_repeat: (GT)26 ; reference: Vienna.990724.curated *F microsatellite_symbol_comment: Why Vienna.990724.curated with two ms with *F same symbol ? *F forward_primer_sequence: cgttcgttcatgggcttttc ; reference: *F Vienna.990724.curated *F forward_primer_sequence_comment: Overlaps reverse_primer of Antp1a *F reverse_primer_sequence: ctctttaatatcggggttgg ; reference: *F Vienna.990724.curated *F temperature: 50 ; reference: Vienna.990724.curated *F . *F \# *F microsatellite_symbol: Antp1a ; reference: FlyBase *F microsatellite_symbol: Antp1 ; reference: Vienna.990724.curated *F microsatellite_symbol_comment: Why Vienna.990724.curated with two ms with *F same symbol ? *F microsatellite_repeat: (GT)26 ; reference: Vienna.990724.curated *F forward_primer_sequence: taatatcggggttgggggtg ; reference: *F Vienna.990724.curated *F reverse_primer_sequence: gttcgttcatgggcttttct ; reference: *F Vienna.990724.curated *F reverse_primer_sequence_comment: Overlaps forward_primer of Antp1 *F temperature: 55 ; reference: Vienna.990724.curated *F IT IS THE SAME LOCUS, HENCE SAME NAME (ANTP1 AND ANTP1A) *F . *F gene: FBgn0020307:dve ; reference: FlyBase *F cytogenetic_position: 58C2--5 ; reference: Vienna.990724.curated *F cytogenetic_position: ?? 58D1--2 ; reference: FBrf0100569 *F nucleic_ac_xfref: G00411 ; reference: FBrf0100569 *F pcr_product_size: 158-162 ; reference: Vienna.990724.curated *F pcr_product_size: 159-163 ; reference: FBrf0100569 *F number_of_alleles: 3 ; reference: Vienna.990724.curated *F observed_heterozygosity: 0.40 ; reference: Vienna.990724.curated *F variance: 0.23 ; reference: Vienna.990724.curated *F THE CHROMOSOMAL POSITION ON OUR WEB PAGE WAS TAKEN FROM AN OLD DATABANK *F ENTRY. WE WILL CORRECT OUR WEBPAGE *F . *F \# *F microsatellite_symbol: 6744 ; reference: Vienna.990724.curated *F microsatellite_repeat: (CT)8 ; reference: Vienna.990724.curated *F microsatellite_repeat: (GA) ; reference: FBrf0100569 *F forward_primer_sequence: cgctaagagtcgctctccat ; reference: *F Vienna.990724.curated *F forward_primer_sequence: ?? cgctctccatccccatctc ; reference: FBrf0100569 *F reverse_primer_sequence: aaattgtttgcccgtctcac ; reference: *F Vienna.990724.curated *F reverse_primer_sequence: ?? tgcttgacggctacataaac ; reference: FBrf0100569 *F temperature: 56 ; reference: Vienna.990724.curated *F temperature: 54 ; reference: FBrf0105317 *F temperature: 54 ; reference: FBrf0100569 *F gene: FBgn0001994:crp ; reference: FlyBase *F cytogenetic_position: 35E6--F5 ; reference: Vienna.990724.curated *F nucleic_ac_xfref: G01323 ; reference: FBrf0100569 *F pcr_product_size: ?? 90-94 ; reference: Vienna.990724.curated *F pcr_product_size: 191-193 ; reference: FBrf0100569 *F number_of_alleles: 3 ; reference: Vienna.990724.curated *F number_of_alleles: 2 ; reference: FBrf0100569 *F observed_heterozygosity: 0.26 ; reference: Vienna.990724.curated *F variance: 0.14 ; reference: Vienna.990724.curated *F gene: Dsec\crp ; reference: FlyBase *F number_of_alleles_sechellia: 2 ; reference: Vienna.990724.curated *F observed_heterozygosity_sechellia: 0.14 ; reference: Vienna.990724.curated *F variance_sechellia: 0.07 ; reference: Vienna.990724.curated *F pcr_product_size_sechellia: ?? 92-94 ; reference: Vienna.990724.curated *F pcr_product_size_sechellia: 191-196 ; reference: FBrf0100569 *F I DO NOT UNDERSTAND YOUR COMMENTS: *F I SEARCHED GENBANK AND FOUND 100% MATCH BETWEEN OUR PRIMER SEQUENCES AND *F THE SEQUENCE GIVEN IN GENBANK. HOWEVER, THE CHROMOSOMAL POSITION DIFFERED *F SLIGHTLY, WHICH MAY RESULT FROM THE FACT THAT WE DID NOT USED THE CLONE *F DS02740, BUT A STS TO DESIGN THE PRIMERS. *F . *F microsatellite_symbol: DS06335a ; reference: Cornell.990725.curated *F microsatellite_repeat: (AC)7 ; reference: Cornell.990725.curated *F microsatellite_repeat: (GT)15 ; reference: Vienna.990724.curated *F microsatellite_repeat: (GT) ; reference: FBrf0096241 *F microsatellite_repeat: (GT) ; reference: FBrf0100569 *F microsatellite_repeat: (AC) ; reference: FBrf0100631 *F forward_primer_sequence: actgtaattgctgttctatgt ; reference: *F Cornell.990725.curated *F reverse_primer_sequence: ctcacactgggacacaaaa ; reference: *F Cornell.990725.curated *F reverse_primer_sequence: ?? cgcacactgggacacaaaa ; reference: *F Vienna.990724.curated *F reverse_primer_sequence_comment: Single bp difference between *F Cornell.990725.curated, FBrf0100631 vs Vienna.990724.curated. *F temperature: 53 ; reference: Cornell.990725.curated *F MgCl2_concentation_mM: 1.5 ; reference: FBrf0100631 *F OUR SEQUENCE IS CORRECT *F __________________________________________________________________ *F Christian Schloetterer *F Institut fuer Tierzucht und Genetik *F Veterinaermedizinische Universitaet Wien *F Josef Baumann Gasse 1 *F 1210 Wien, Austria *F phone: +43-1-25077-5603 (office) *F +43-1-25077-5614 (lab) *F fax: +43-1-25077-5693 *F http://i122server.vu-wien.ac.at/ # *U FBrf0110766 *a Sekelsky *b J. *t 1999.6.22 *T personal communication to FlyBase *u *F >From sekelsky@email.unc.edu Tue Jun 22 17:27:32 1999 *F >To: rd120@gen.cam.ac.uk *F >Subject: UDPGT *F > *F >Hi Rachel. Scott forwarded your request about info on mei-P44 and *F >UDP-glucuronoslytransferase. I took another look at the sequence. It *F >seems to be a more complicated story than we thought when we submitted the *F >paper. The 86D P element is at 71,928 (first base of the 8-bp target *F >sequence) on the current AC006491 (BACR48M21) sequence. It seems there are *F >about a dozen UDP-GLUCURONOSYLtransferase and/or UDP-GLUCOSYLtransferase *F >genes between about 65,000 and 93,000 of this sequence, on both strands. *F >It now looks like our insertion is between two of them, rather than within *F >an intron of one. Two of these dozen sequences correspond to recently *F >submitted Drosophila glucosyltransferase sequences (Genbank AF116554-5). *F >One of the authors of that submission is one of the authors on the '96 fly *F >meeting abstract. You could perhaps contact him for more information on *F >whether these genes have additional designations. *F > *F >Good luck. *F > *F >Jeff Sekelsky # *U FBrf0110770 *a Seshaiah *b P. *c D.J. *d Andrew *t 1999.7.29 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Jun 01 10:51:19 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 1 Jun 1999 10:51:19 +0100 *F To: dandrew@jhmi.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 1 Jun 1999 10:54:52 +0100 *F Content-Length: 966 *F Dear Dr. Andrew, *F I am curating your paper for FlyBase: *F Seshaiah and Andrew, 1999, Molec. Biol. Cell 10(5): 1595-1608 *F In this paper you look at the expression of all 20 aminoacyl-tRNA *F synthetases. *F You state that you found Berkeley EST's which correspond to each of *F these tRNA synthetases. It would be very helpful to FlyBase if you *F could tell me the number of each EST (e.g. LD10023) which corresponds *F to each particular tRNA synthetase. This information would be recorded *F as a personal communication from you to FlyBase, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From dandrew@bs.jhmi.edu Thu Jul 29 14:50:39 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 29 Jul 1999 14:50:39 +0100 *F Date: 29 Jul 99 09:58:15 -0400 *F From: Debbie Andrew *F Subject: tRNA synthetases *F To: Gillian Millburn *F X-Mailer: QuickMail Pro 1.5.3b3 (Mac) *F X-Priority: 3 *F MIME-Version: 1.0 *F Reply-To: Debbie Andrew *F Content-Type: multipart/mixed; boundary='====49545055494957554950===1' *F Content-Length: 2415 *F Dear Gillian, *F Sorry for taking so long to get this information to you--its the info on which Berkeley ESTs correspond to the 20 AA-tRNA synthetases. The information should be recorded as a personal communication from both me and the graduate student who did the work (Partha Seshaiah and Deborah J. *F The information is in the enclosure. *F Cheers, *F Debbie *F Deborah J. Andrew *F Dept. of Cell Biology and Anatomy *F The Johns Hopkins University School of Medicine *F 725 N. Wolfe St. *F Baltimore, MD 21205-2196 *F Ph: 410-614-2722 *F FAX: 410-955-4129 *F email: dandrew@jhmi.edu *F ClassI *F ArgRS GM08880 *F CysRS LD07625 *F GlnRS LD25392 *F GluRS LD14109 *F IleRS LD11930 *F LeuRS GM06905 *F MetRS LD07463 *F TrpRS GH06221 *F TrpRS LD24552 *F TyrRS GM09071 *F ValRS GM09906 *F ClassII *F AlaRS LD07142 *F AsnRS GM04706 *F AspRS LD24891 *F GlyRS GM01134 *F HisRS GM05203 *F LysRS LD13687 *F PheRS LD05193 *F ProRS GM03563 *F SerRS HL02233 *F ThrRS LD06190 # *U FBrf0110939 *a Tiong *b S. *t 1999.8.10 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Stanley Tiong, Exelixis Pharmaceuticals Inc. *F To: Bloomington Drosophila Stock Center *F Subject: Df(3R)urd breakpoints *F Dated: 9 August 1999 *F *F Background: Some confusing complementation results with Df(3R)urd and P alleles led Helen Francis-Lang to ask Stanley Tiong to examine a polytene squash of Df(3R)urd. *F *F Information communicated: *F *F The breakpoints of Df(3R)urd are 87F9-10;88B01-02. # *U FBrf0111015 *a Wakimoto *b B. *t 1999.6.23 *T personal communication to FlyBase *u *F Date: Wed, 23 Jun 1999 21:15:11 -0700 (PDT) *F From: Barbara Wakimoto *F To: Leyla Bayraktaroglu *F Subject: Re: homer *F Hi Leyla, *F sorry to be so slow in getting back to you on this. Our homer is *F indeed a different entity from Xiao's homer. In order to eliminate the *F redundancy, we'll rename our homer since the published homer has *F precedence. We've decided to rename our gene 'kugi' *F instead, a Japanese name for nail, the shape of the sperm head in this *F paternal effect mutant. Can you record this change for us in FlyBase? *F Thanks, *F Barbara *F On Wed, 19 May 1999, Leyla Bayraktaroglu wrote: *F > Dear Dr. Wakimoto, *F > *F > There are two 'homer's in FlyBase, *F > 'homr' from your lab (Dros. Research Conf. 40:743A) and *F > 'homer' from Xiao et al. (Neuron 1998 21(4):707--716). I wanted *F > to make sure that these are indeed two distinct entities (they *F > do seem to be.) Can you confirm this for me? *F > *F > Thanks, *F > *F > Leyla Bayraktaroglu *F > Curator, FlyBase *F > leyla@morgan.harvard.edu # *U FBrf0111201 *a Casanova *b J. *t 1999.9.6 *T personal communication to FlyBase *u *F # *U FBrf0111202 *a Schupbach *b G. M. *t 1999.9.20 *T personal communication to FlyBase *u *F Date: 20 Sep 99 18:49:01 -0400 *F From: Gertrud M Schupbach *F Subject: RE: question from FlyBase *F To: Beverley Matthews *F *F Reply to: RE: question from FlyBase *F Dear Beverley, *F *F Well, I actually was able to go back to Robert Clifford's thesis, which has more *F data than what was published. In his thesis, he actually says that the basepair *F change affects bp 2469 and amino acid Cys 655 to Ser. According to your email *F this would make more sense. I have no idea why in the Table 2 of his paper Cys *F 662 is listed. The only thing I can imagine is that a typo was "corrected" along *F the way, making matters worse. Anyway, it should be 655 from all I can tell. And *F by the way, we also sent in another correction to the sequence in GenBank in *F recent years. But you probably know all that. *F Best wishes. *F Yours, Trudi. *F *F *F >Date: Mon, 20 Sep 1999 11:40:47 -0400 (EDT) *F >From: Beverley Matthews *F >Subject: question from FlyBase *F >To: gschupbach@molbio.princeton.edu *F *F >Dear Dr. Schupbach, *F > *F >I am a curator for FlyBase and am working on the annotation of the *F >Drosophila genome. I was marking the sites of mutations of Egfr and ran *F >across an ambiguity. Egfr[f37] (referred to as Egfr[4A] in your '94 *F >Clifford and Schupbach Genetics paper (137:531-550) is marked as having *F >a change from TGC to AGC at genomic position 2469 that leads to amino *F >acid change Cys 662 to Ser. The problem is that genomic position 2469 *F >does not correspond to amino acid 662. The change may have been at 2469 *F >(amino acid 655) or it may have been at 2490 (amino acid 662). The same *F >TGC codon occurs in both places. I'm not sure whether the nucleotide *F >position or the amino acid position was the typo. *F *F >I know it can be very difficult to track down details like this years *F >after the experiments were done but if you have any way of checking or *F >can tell me who to ask, I'd love to get the correct information for *F >the annotation. *F *F >Thanks for your help, *F >Sincerley, *F > *F >Beverley Matthews *F >FlyBase-Harvard *F # *U FBrf0111203 *a Bloomington Drosophila Stock Center *b ?. *t 1999.9.22 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase from Kevin Cook, 22 Sept. 1999 *F *F The stock center has obtained a homozygous viable and fertile X *F chromosome insertion of P{w[+mC]=UAS-dally.J} [FBal0083022]. The insertion *F originated from the work described in Jackson et al. 1997 [FBrf0099288] and *F the source for the P localization is Larry Marsh, UC Irvine. *F *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F # *U FBrf0111204 *a Bloomington Drosophila Stock Center *b ?. *t 1999.9.23 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase from Kevin Cook, 23 Sept. 1999 *F *F The Bloomington Stock Center has obtained a viable second chromosome *F insertion of P{w[+mC]=dj-GFP.S}. The construct and its transformation are *F described in Santel, A. et al. 1997 Mech. Dev. 64(1-2): 19--30 [FBrf0096230]. *F *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F *F # *U FBrf0111591 *a Chien *b C.T. *t 1999.9.15 *T personal communication to FlyBase *u *F > Dear Dr. Yamada: *F > *F > The asolo gene that I mentioned in my abstract ID65 is now renamed to amos. *F > This gene is also discoverd by A. Jarman's laborotory in U. of Edinburgh. *F > Their original name was rolo. Right now both sides agree to use the name, *F > amos. You can confirm with Dr. Jarman. The map position is at the junction *F > of 36E and 36F by polytene in situ hybridization. It is uncovered by *F > deficiencies TW203 and M36F-S5. Both fly strains are from Bloomington Stock *F > Center. Let me know if you more information from me! *F > *F > Cheng-Ting Chien, Ph. D. *F > *F > Institute of Molecular Biology *F > Academia Sinica *F > 128 Sec#2 Academia Road *F > Nankang, Taipei 11529 *F > Taiwan *F > Tel: 886-2-27899193 *F > Fax: 886-2-27826085 *F > *F Subject: EDRC99 - abstract ID65 *F e-mail: ctchien@ccvax.sinica.edu.tw *F author: Chien, C.-T. *F Dear Dr. Chien, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'The proneural gene asolo promotes multiple dendritic neuron formation *F in Drosophila peripheral nervous system' *F You mention a gene that is new to FlyBase, asolo. *F Do you have a map location for asolo? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Chihiro # *U FBrf0111592 *a Heitzeberg *b F. *t 1999.9.15 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID423 *F e-mail: Fabian@genetik.biologie.fu-berlin.de *F author: Heitzeberg, F. *F Dear Dr. Heitzeberg, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'The ATF-3-like bZIP-transcription factor A3-3 of Drosophila *F melanogaster is involved in development and female fertility.' *F You mention a gene that is new to FlyBase, A3-3. *F Is this gene different from the 'Bsg25D' gene (at 25D) which also has *F homology to the human fos gene ? *F Bsg25D has been described in: *F Boyer et al., 1987, Nucleic Acids Res. 15: 2309--2325 *F George and Terracol, 1997, Genetics 146(4): 1345--1363 *F If A3-3 is a new gene, do you have map location for it? It is nice if *F we can keep as many gene records as possible anchored to the map. *F Also, FlyBase has a record of a number of enhancer trap lines with *F names like A3-3-25, A3-3-47 etc. Was A3-3 first identified using one of *F these lines ? *F Finally, do you have an allele designation for the mutation, e.g. *F A3-31 ( = superscript) If so then we can store the information *F about it much more precisely. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F > Dear Dr. Chihiro Yamada! *F > 1. A3-3 is not an allel of Bsg25D *F > *F > 2. The location of A3-3 is X-1F *F > *F > 3. The A3-3 enhancer trap line was screened and analysed in our lab. There *F > is no relation to A3-3-25 and A3-3-47. A3-3 is a temporary lab name. We *F > will change the name as soon as the function of the gene is better known. *F > *F > 4. wild type allel: A3-3 mutation: A3-31 = (superscript) *F > *F > with best wishes *F > Fabian Heitzeberg *F > # *U FBrf0111593 *a Zinke *b I. *t 1999.9.15 *T personal communication to FlyBase *u *F > *F > Dear Dr. Yamada, *F > the map location of ppl is 78C. In the abstract we talk about one *F > allele, ppl(06913). But we have also more alleles of the ppl-gene. A *F > paper on the ppl-gene is in press (Zinke et al., Development). *F > If you have further questions, feel free to contact us. *F > Sincerly *F > Ingo *F > *F > *F Subject: EDRC99 - abstract ID372 *F e-mail: ingo.zinke@igen.fzk.de *F author: Zinke, I. *F Dear Dr. Zinke, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Control of food intake and growth in Drosophila larva by pumpless, a *F gene encoding an enzyme involved in amino acid metabolism' *F You mention a gene that is new to FlyBase, ppl. *F Do you have a map location for ppl? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Also, from your abstract it seems to me that you are talking about one *F particular mutation rather than several alleles of the same gene, is *F this correct ? Do you have an allele designation for the mutation, e.g. *F ppl1 ( = superscript) If so then we can store the information about *F it much more precisely. *F Thank you for your help, *F with best wishes, *F Chihiro # *U FBrf0111594 *a Ekengren *b S. *t 1999.9.15 *T personal communication to FlyBase *u *F > From sophia@molbio.su.se Wed Sep 15 14:17:47 1999 *F > Envelope-to: cy200@gen.cam.ac.uk *F > Delivery-date: Wed, 15 Sep 1999 14:17:47 +0100 *F > Mime-Version: 1.0 *F > X-Sender: sophia@nyctea.molbio.su.se *F > Date: Wed, 15 Sep 1999 15:22:35 +0200 *F > To: Chihiro Yamada *F > From: sophia@molbio.su.se *F > Subject: Re: EDRC99 - abstract ID324 *F > *F > Dear Dr. Chihiro Yamada *F > According to your request. *F > I have localized the gene to band 93A1-2 on the 3:rd chromosome. *F > After looking into the dictionary I also (visely I think) decided to have *F > the abbrivation Tot (instead of Turt) for Turandot... *F > Sincerely yours, *F > Sophia Ekengren *F > *F Subject: EDRC99 - abstract ID324 *F e-mail: sophia@molbio.su.se *F author: Ekengren, S. *F Dear Dr. Ekengren, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Turandot, a novel peptide bridging immune- and stress response in *F Drosophila melanogaster' *F You mention a gene that is new to FlyBase, Turt. *F Do you have a map location for Turt? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0111595 *a Deak *b P. *t 1999.9.15 *T personal communication to FlyBase *u *F Dear Chihiro, *F Indeed, mákos (mks) corresponds to cdc27. This gene was previously *F identified at cDNA level only. Now, mks has two mutant alleles, mks1 *F and mks2. mks1 was identified from our P collection and its *F identifier is l(3)092309. mks2 is a P-lacW insertion allele as mks1 *F but it comes from the BDGP and its available from Bloomington. The *F identifier number of mks2 is l(3)L7123 and the stock number is P168. *F The plasmid rescue sequences from these lines overlap with each other *F and with the incomplete cDNA sequence (U18298) in the database. The *F insertion has been localized to the 5'-UTR in both alleles and they are *F about 250 bp apart from each other, mks2 being closer to the 5'-end of *F the transcript. *F mks1 is recessive pharate adult lethal *F mks2 is recessive semilethal and female sterile *F In double mutant combinations, they show genetic interaction with *F several mitotic mutations, such as tws(or aar), polo, string, asp and *F bub1. *F There is a noticeable discrepancy between the localization of mks1 and *F l(3)L7123. I mapped mks1 to 65E7-12 but according to FlyBase, *F l(3)L7123 was mapped to 65F1-2. Because of that, I double checked my *F preparations and photos and I'm quite sure that the insertion is in 65E *F (the in situ signal and the 65F1-2 double band appear separated on well *F spread chromosomes and the signal is distal from 65F1-2. *F Cheers, *F Peter *F Hiya Peter, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Characterization of makos, a new mitotic gene encoding a subunit of *F the anaphase promoting complex in Drosophila melanogaster' *F Could you confirm that makos is the same as the gene in 'Cdc27' gene we already have in FlyBase (accession numbers: U18298, Q24021). I'm pretty sure they are the same, but just wanted to check. *F Also, what is the identifier number of the P-element mutation in makos *F \- is it one of the Szeged lines from your paper Deak et al., 1997, *F Genetics 147(4): 1697--1722? *F Thank you for your help, *F with best wishes, *F Chihiro # *U FBrf0111596 *a Struenkelnberg *b M. *t 1999.9.15 *T personal communication to FlyBase *u *F From martin@whisky.biologie.uni-freiburg.de Fri Sep 17 14:50:47 1999 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Fri, 17 Sep 1999 14:50:47 +0100 *F Date: Fri, 17 Sep 1999 15:56:32 +0200 (MDT) *F From: Martin Struenkelnberg *F To: Chihiro Yamada *F Subject: Re: EDRC99 - abstract ID119 (fwd) *F MIME-Version: 1.0 *F Dear Mr. Chihiro Yamado, *F . *F . *F Put in that kirre maps to 3C somewhere 5' of Notch, *F please. That would be cool. *F . *F . *F I will get back to you when I rechecked everything. Thank you *F very much! *F Yours, Martin. *F \-------------------------------------------------------------------------------- *F Martin Struenkelnberg *F email: struenke@mibm.ruf.uni-freiburg.de *F oder : martin@whisky.biologie.uni-freiburg.de *F Homepage: http://whisky.biologie.uni-freiburg.de/~martin/ *F \-------------------------------------------------------------------------------- *F On Thu, 16 Sep 1999, Chihiro Yamada wrote: *F > Dear Martin, *F > *F > Thank you for your prompt reply. I will enter your map data as a *F > personal communication, If thats O.K with you. The cDNA sequence can *F > be submitted to GENBANK if you wish, and as a consequence will work its *F > way into FlyBase records. As far as expression data are concerned, we *F > prefer to wait for them to be published, in order to avoid having to *F > curate them twice. *F > *F > Best Wishes, *F > *F > Chihiro *F > *F > *F > > Dear Dr. Yamada, *F > > *F > > the genomic sequence of kirre has been sequenced during the European *F > > Drosophila genome project and is located in *F . *F > >region 3C on the distal X-chromosome. *F . *F Notch is located .. 3' of kirre. The gene was denoted *F > > kirre, because it shows a very high homology to irreC-rst, or irre, as we *F > > usually call it. *F > > *F > > We also have expression data and, of course, a complete cDNA sequence. Can *F > > I submit that as well? *F > > *F > > Thank you very much, *F > > Martin. *F > > *F > > *F > > *F > > > ---------- Forwarded message ---------- *F > > > Date: Tue, 14 Sep 1999 18:03:46 +0100 *F > > > From: Chihiro Yamada *F > > > To: martin@whisky.biologie.uni-freiburg.de *F > > > Subject: EDRC99 - abstract ID119 *F > > > *F > > > Subject: EDRC99 - abstract ID119 *F > > > e-mail: martin@whisky.biologie.uni-freiburg.de *F > > > author: Struenkelnberg, M. *F > > > *F > > > Dear Dr. Struenkelnberg, *F > > > *F > > > We are currently curating the abstracts for the upcoming European *F > > > Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F > > > connection with your abstract: *F > > > *F > > > 'Kirre, a novel transmembrane Immunoglobulin protein, is highly related *F > > > to the IrreC-rst neural cell adhesion molecule.' *F > > > *F > > > You mention a gene that is new to FlyBase, kirre. *F > > > *F > > > Do you have a map location for kirre? It is nice if we can keep as *F > > > many gene records as possible anchored to the map. *F > > > *F > > > Thank you for your help, *F > > > *F > > > with best wishes, *F > > > *F > > > Chihiro *F > > > ---------------------------------------------------------------------- *F > > > Chihiro Yamada. *F > > > *F > > > FlyBase (Cambridge), *F > > > Department of Genetics, *F > > > University of Cambridge, *F > > > Downing Street, email: c.yamada@gen.cam.ac.uk *F > > > Cambridge, CB2 3EH, Ph : 01223-333963 *F > > > UK. FAX: 01223-333992 *F > > > ---------------------------------------------------------------------- *F > > > *F > > > *F > > > *F > > *F > # *U FBrf0111597 *a Davies *b J. *t 1999.9.16 *T personal communication to FlyBase *u *F >Subject: EDRC99 - abstract ID163 *F >e-mail: j.a.davies@sussex.ac.uk *F >author: Davies, J.A. *F > *F >Dear Dr. Davies, *F > *F >We are currently curating the abstracts for the upcoming European *F >Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F >connection with your abstract: *F > *F >'Two Drosophila innexins co-operate to form heteromeric gap junction *F >channels' *F > *F >You mention 2 innexin genes that are new to FlyBase, Ix2 and Ix3. *F > *F >Do you have a map location for Ix2 and Ix3? It is nice if we can keep *F >as many gene records as possible anchored to the map. *F > *F >Also, are these two genes different from shakB (at 19E) and ogre (at *F >6E), which are also innexins ? *F > *F >Thank you for your help, *F > *F >with best wishes, *F > *F >Chihiro *F Dear Chihiro *F 1. We have decided not to use the suffix ix to designate these genes, but *F to use inx instead. There have been a couple of recent publications that *F use this abbreviation and this is what the worm people do, too. The genes *F are now inx2 and inx3. *F 2. They are different from shak and ogre. inx2 is the same as prp33 (Curtin *F et al; Gene 232: 191-201.) We have used the nomenclature inx2 for this gene *F because the nomenclature is getting confusing as more members of this gene *F family are being discovered and for the reasons below: *F Ganforina et al (1999) have recently presented a Schistocerca americana *F protein, Sa-Inx(1), which is an orthologue of Drosophila Ogre. In the light *F of this, Dm-Inx1 must now be considered as an alternative nomenclature for *F Ogre. Additionally, Ganfornina et al have identified another grasshopper *F innexin, Sa-Inx(2). Subsequently, Curtin et al (1999) isolated a gene *F encoding its Drosophila orthologue (prp33). However, a partial sequence *F corresponding to this Drosophila gene had already been deposited in the *F BDGP EST database and it was from here that we obtained the cDNA clone for *F inx2. To keep the nomenclature congruent with other organisms and to ease *F cross-species comparisons, we decided that inx2 was the best nomenclature *F for this gene. inx3 which was also identified in the BDGP database has no *F known orthologues in other organisms. *F I'm sure that this is more detail than you want, but we are anxious to *F prevent the gratuitous proliferation of duplicate and confusing names for *F these loci, and it seems to us the best way is to put in place a logical *F system of nomenclature ASAP. This is discuseed in our forthcoming paper (J. *F Cell Biol., in press) and has been touched on in our TIGS review (TIGS 14: *F 348-349, 1998) *F 3. The locations are: *F inx2 - 6E4-5. This maps to cosmid 17F90 *F inx3 - 98E4-6. The P1 clones DS03216 & DS04968 contain inx3 *F The genes and their characterisation are described in Stebbings et al., J. *F Cell Biol. in press. *F Please get in touch if you need any more information *F Yours, *F Jane Davies *F Dr Jane Davies *F Sussex Centre for Neuroscience *F Sussex University *F Falmer *F Brighton BN1 9QG *F UK # *U FBrf0111599 *a Loppin *b B. *t 1999.9.16 *T personal communication to FlyBase *u *F >Subject: EDRC99 - abstract ID123 *F >e-mail: loppin@maccgmc.univ-lyon1.fr *F >author: Loppin, B. *F > *F >Dear Dr. Loppin, *F > *F >We are currently curating the abstracts for the upcoming European *F >Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F >connection with your abstract: *F > *F >'sesame, a maternal effect mutation affecting male pronucleus maturation *F >in Drosophila melanogaster' *F > *F >You mention a gene that is new to FlyBase, ssm. *F > *F >From your abstract it looks as though you are talking about a single *F >allele, is this the case ? Do you have an allele designation for the *F >ssm mutant, like ssm1? If so then we can store the information about *F >it much more precisely. *F > *F Dear Chihiro, *F We have at the moment a single EMS allele of the sesame (ssm) gene which is *F not cloned. I propose you to call it ssm185b for it was first isolated as *F fs(1)185b (Tatout et al., Int. J. Dev. Biol. 38: 27-33 (1994)). *F Best regards, *F Benjamin Loppin. *F CNRS UMR 5534 *F Centre de Génétique Moléculaire et Cellulaire *F Université Claude Bernard, Lyon I *F 43,Boulevard du 11 Novembre 1918, Bâtiment 741 *F 69622 VILLEURBANNE CEDEX, FRANCE *F Tel (33) (0)4 72 44 80 00 poste 3372 *F Fax : (33)(0)4 72 44 05 55 # *U FBrf0111600 *a Hoehne *b M. *t 1999.9.22 *T personal communication to FlyBase *u *F Dear Chihiro, *F the gene we call reggie-2 is identical to the already published gene *F flotillin-1 (Galbiati et al., Gene 210:229-237 (1998)). We decided to *F stick to the name reggie-2 since the homologous sequence (from Carassius *F auratus) was first published as reggie-2 (Schulte et al., Development *F 124:577-587 (1997)) and this name indicates the gene's function during *F neural regeneration (at least in the fish). *F Galbiati et al. also mention a gene they call flotillin-2 which might be *F identical to reggie-1, but we believe that reggie-1 codes for a protein *F 49aa longer (N-terminally) than the published flotillin-2. Our *F chromosomal-in-situ-hybridizations suggest that reggie-1 is located on the *F X-chromosom (region 13). *F Sincerely yours, *F Martin *F On Tue, 14 Sep 1999, Chihiro Yamada wrote: *F > Subject: EDRC99 - abstract ID253 *F > e-mail: marthoe@biologie.uni-freiburg.de *F > author: Hoehne, M. *F > *F > Dear Dr. Hoehne, *F > *F > We are currently curating the abstracts for the upcoming European *F > Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F > connection with your abstract: *F > *F > 'Characterization of Drosophila homologues of reggie, a gene family *F > associated with neural regeneration in vertebrates' *F > *F > You mention 2 genes that are new to FlyBase, reggie-1 and reggie-2. *F > *F > Do you have a map location for reggie-1 and reggie-2? It is nice if we *F > can keep as many gene records as possible anchored to the map. *F > *F > Also, do you have symbols for these genes ? Reg-2 is already in use *F > (for 'Rhythmically expressed gene 2' and Reg-1 (for 'Rhythmically *F > expressed gene 1') is a synonym for Adh, however, rgg-1 and rgg-2 are *F > not in use. *F > *F > Thank you for your help, *F > *F > with best wishes, *F > *F > Chihiro # *U FBrf0111601 *a Baum *b B. *t 1999.9.26 *T personal communication to FlyBase *u *F Dear Chihiro *F The gene I work on is a homologue of cap, I named it capulet. *F Since arriving back I find a P-element adjacent to Dcap was published in *F the Rubin paper (showing P element locations). *F It its at 21F, 2 allelic Ps are P367 and P2330 (stock numbers) *F Hope this is useful. *F Buzz *F Buzz Baum, *F Dept. of Genetics, *F Havard Med. School, HHMI, *F 200 Longwood Ave., *F Boston, *F MA 02115. *F tel:617 432 7548 *F fax:617 432 7688 *F Subject: EDRC99 - abstract ID200 *F e-mail: bbaum@rascal.med.harvard.edu *F author: Baum, B. *F Dear Dr. Baum, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Cap controls the global cellular organisation of actin filaments and *F is required for the correct polarity of the Drosophila oocyte' *F You mention a gene that is new to FlyBase, capulet. *F Do you have a map location for capulet? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Also, the symbol you use for capulet is already in use for another *F gene: *F 'Cap' = Chromosome-associated protein and 'cap' = capon *F do you have another symbol for this gene - 'capl' is not in use for *F example, *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0111602 *a Riesgo-Escovar *b J. *t 1999.9.23 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID98 *F e-mail: juan@zool.unizh.ch *F author: Riesgo-Escovar, J. *F Dear Dr. Riesgo-Escovar, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'piragua, a new gene involved in dorsal closure in Drosophila.' *F You mention a gene that is new to FlyBase, piragua. *F Do you have a map location for piragua? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Chihiro *F Dear Dr. Yamada: *F Indeed, I think we are characterizing a new gene, but unfortunately at *F the present time we have not yet confirmed rigorously its precise *F location. As a consequence, we would not like to venture that *F information now, because we could still be wrong. The gene is on the *F second chromosome, on 2L, according to our data, but we are still not *F sure as to its precise location. Hope you understand. As soon as we *F get definitive proof we will contact you. *F Sincerely, *F Dr. Juan R. Riesgo-Escovar # *U FBrf0111603 *a Monge *b I. *t 1999.9.24 *T personal communication to FlyBase *u *F From: Ignacio Monge *F Subject: Re: EDRC99 - abstract ID137 *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Length: 2039 *F Dear colleague, *F I actually answer to your email already. There was maybe a problem during *F the transfer and it did not come. *F The answer is YES: *F What I have called Df(3L)XS-5R is SR3-7XS-5R. It is the SAME LINE. *F Yours sincerely, *F Ignacio *F >Dear Monge, *F > *F >Just over a week ago, I sent you a query concerning your abstract for *F >the upcoming EDRC99 conference. I was wondering if you'd had a chance *F >to look at it. I enclose the mail below for your convenience. *F > *F >Thanks *F > *F >Chihiro *F > *F > *F >> From cy200 Tue Sep 14 18:05:10 1999 *F >> To: monge@pharma.unizh.ch *F >> Subject: EDRC99 - abstract ID137 *F >> X-Sun-Charset: US-ASCII *F >> Content-Length: 1086 *F >> *F >> Subject: EDRC99 - abstract ID126 *F >> e-mail: monge@pharma.unizh.ch *F >> author: Monge, I. *F >> *F >> Dear Dr. Monge, *F >> *F >> We are currently curating the abstracts for the upcoming European *F >> Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F >> connection with your abstract: *F >> *F >> 'Essential roles for Drosophila transcription factor AP-2 (dAP-2) in *F >>CNS, proboscis and leg development' *F >> *F >> You mention a chromosomal aberration that may be new to FlyBase, *F >> 'Df(3L)XS-5R'. *F >> *F >> We have a record of a mutant called 'SR3-7XS-5R' that was described *F >> in: *F >> *F >> Karim et al., 1996, Genetics 143(1): 315--329 *F >> *F >> do you know if this is the same chromosome ? *F >> *F >> Thank you for your help, *F >> *F >> with best wishes, *F >> *F >> Chihiro *F >> ---------------------------------------------------------------------- *F >> Chihiro Yamada. *F >> *F >> FlyBase (Cambridge), *F >> Department of Genetics, *F >> University of Cambridge, *F >> Downing Street, email: c.yamada@gen.cam.ac.uk *F >> Cambridge, CB2 3EH, Ph : 01223-333963 *F >> UK. FAX: 01223-333992 *F >> ---------------------------------------------------------------------- *F >> *F Ignacio Monge *F Pharmakologisches Institut *F Universität Zürich-Irchel *F Winterthurerstrasse 190 *F CH-8057 Zürich *F Switzerland *F Email: monge@pharma.unizh.ch *F Tel: 41-1-635 59 50 *F Fax: 41-1-635 57 08 # *U FBrf0111604 *a Runk *b A. *t 1999.9.23 *T personal communication to FlyBase *u *F Greetings, thank you for your patience. We are aware that the northern data *F does not coincide with the complementation analysis. However, we are *F currently repeating the northern analysis to determine if the 'extra' *F altered transcript in 2A is just an artifact. If you look at the northern, *F 83A appears to have a stronger signal at 4kb when compared to 2A. The *F complementation study does indeed point out that the mutation in 2A is in a *F different gene from that of 83A. We are confident that the mutation is in *F the 83A mutant. Thus, we are changing the following statement in the *F abstract ( = superscript): *F 'In addition, a 4 kb transcript was seen in both mutant lines suggesting an *F alteration.' *F to *F 'In addition, a 4 kb transcript was seen in the AT7683A mutant, which *F suggests an alteration.' *F We would like to use the symbol Ubc87F, the name Ubiquitin conjugating *F enzyme, and the map location 87F12 for this gene. *F I hope this clears any confusion, *F Alexander Runk *F Delivery-date: Fri, 24 Sep 1999 12:34:58 +0100 *F From: 'Alexander Runk' *F To: 'Chihiro Yamada' *F Subject: RE: EDRC99 - abstract ID140 addendum *F Ok, here's an up-to-date title and abstract that we will be using in the *F conference ( = indentation). *F Analysis of a Novel Muscle Ubiquitin Conjugating Enzyme in Drosophila *F Our goal is to characterize mutations in novel Drosophila genes that *F disrupt the structure and function of the neuromuscular system in a manner *F analogous to the known myopathies in humans. A homozygous enhancer-trap *F line, AT76, was generated resulting in a stable insertion of P{lwB} in the *F genome. Cytogenetic and P1 mapping placed the insertion in the region 87F12. *F These flies display wild-type flight behavior, which is consistent with the *F normal indirect flight muscle (IFM) morphology observed in electron *F micrographs. Excision of the enhancer trap resulted in two recessive lethal *F lines, AT7683A and AT762A, which fall within two complementation groups. *F Furthermore, AT7683A displays dominant flight-defective behavior. The IFM *F ultrastructure of the flight-defective flies is markedly disrupted. We *F isolated a 5.5 kb genomic fragment flanking the insertion, initiated a *F chromosomal walk, and cloned a 531 bp partial cDNA with a predicted amino *F acid sequence that has 75% identity to the human skeletal muscle ubiquitin *F conjugating enzyme, ube2g, an E2 class enzyme. RFLP analysis shows *F chromosomal rearrangement within the region of the cDNA. Northern blots *F probed with this cDNA reveal 850 bp, 1.3 kb, and 2 kb transcripts. In *F addition, a 4 kb transcript was seen in the AT7683A mutant, which suggests *F an alteration. A 9 kb genomic fragment containing this putative gene was *F cloned into pP{CaSpeR} to perform germline transformations. Currently, we *F are analyzing the transformants to determine whether the mutant phenotypes *F have been rescued. *F Sincerily, *F Alexander Runk *F \-----Original Message----- *F From: Chihiro Yamada mailto:cy200@gen.cam.ac.uk *F Sent: Thursday, September 23, 1999 1:57 AM *F To: arunk@mail.sdsu.edu *F Subject: Re: EDRC99 - abstract ID140 *F Dear Runk, *F Just over a week ago, I sent you a query concerning your abstract for *F the upcoming EDRC99 conference. I was wondering if you'd had a chance *F to look at it. I enclose the mail below for your convenience. *F Thanks *F Chihiro *F > From cy200 Tue Sep 14 18:06:03 1999 *F > To: arunk@mail.sdsu.edu *F > Subject: EDRC99 - abstract ID140 *F > X-Sun-Charset: US-ASCII *F > Content-Length: 1659 *F > *F > Subject: EDRC99 - abstract ID140 *F > e-mail: arunk@mail.sdsu.edu *F > author: Runk, A. *F > *F > Dear Dr. Runk, *F > *F > We are currently curating the abstracts for the upcoming European *F > Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F > connection with your abstract: *F > *F > 'Analysis of a Novel Muscle-Specific Ubiquitin-Conjugating Enzyme in *F > Drosophila' *F > *F > In you abstract you talk about two lines derived from the enhancer trap *F line AT76, which you call 'AT7683A' and 'AT7683A' ( = superscript). *F > *F > You state that these 'fall within two complementation groups.' which *F > suggests that they are in two separate genes. However, later in you *F > abstract you state 'a 4 kb transcript was seen in both mutant lines *F > suggesting an alteration in the mRNA.' which suggests that the two *F > lines are mutants in the same gene. *F > *F > Could you confirm whether these two mutant lines are mutations within *F > the same gene or two different ones. *F > *F > Also, for 'AT7683A' you state that the mutation is in a homologue of *F > 'human ubiquitin-conjugating enzyme (ube2g)'. Would you like the *F > symbol 'Ube2g' to be the valid symbol for this gene in FlyBase, or do *F > you have an alternative symbol for the gene that is mutated in *F > 'AT7683A' ? *F > *F > Thank you for your help, *F > *F > with best wishes, *F > *F > Chihiro *F > ---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: c.yamada@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > ---------------------------------------------------------------------- *F > # *U FBrf0111605 *a Hirai *b K. *t 1999.9.24 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID76 *F e-mail: hirai@drochan.bio.kit.ac.jp *F author: Hirai, K. *F Dear Dr. Hirai, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Cytogenetic characterization of a novel meiotic mutant mei-yh92 in the *F male of Drosophila melanogaster' *F You mention a gene that is new to FlyBase, mei-yh92. *F Do you have a map location for mei-yh92? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Do you have an allele designation for the mei-yh92 mutant, like *F mei-yh921? If so then we can store the information about it much *F more precisely. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Dear Chihiro Yamada, *F Thank you for your message. *F > 'Cytogenetic characterization of a novel meiotic mutant mei-yh92 in the *F > male of Drosophila melanogaster' *F > You mention a gene that is new to FlyBase, mei-yh92. *F > Do you have a map location for mei-yh92? It is nice if we can keep as *F > many gene records as possible anchored to the map. *F The mei-yh92 gene is on chromosome 2, but the precise location is not known now. *F > Do you have an allele designation for the mei-yh92 mutant, like *F > mei-yh921? If so then we can store the information about it much *F > more precisely. *F Yes, it is the original allele, so the designation is mei-yh921. *F Best wishes, *F Kazuyuki Hirai *F \-- *F Kazuyuki HIRAI *F Genetics Lab., Section of Biofunctions, *F Kyoto Institute of Technology *F Matsugasaki, Kyoto 606-8585, JAPAN *F e-mail: hirai@drochan.bio.kit.ac.jp *F phone : +81-75-724-7797 *F fax : +81-75-724-7760 # *U FBrf0111606 *a Fasulo *b B. *t 1999.9.15 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID298 *F e-mail: bfasulo@axcasp.caspur.it *F author: Fasulo, B. *F Dear Dr. Fasulo, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'doppio fuso (duo), a gene required for spindle pole assembly during *F Drosophila male meiosis' *F You mention a gene that is new to FlyBase, duo. *F Do you have a map location for duo? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Also, you state that the P-element causing the duo mutation 'lies in *F the 5' untranslated region of a gene that encodes a poly-A-binding *F protein'. *F FlyBase has a record of 3 genes encoding a poly-A-binding protein. *F These are 'pAbp' (at 55B), 'Rox2' (also known as 'polyA-binding protein *F II', at 44B), 'Rox8' (at 95D). Is the duo mutation in one of these 3 *F genes, or is it in a new poly-A-binding protein ? *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Dear Dr. Yamada, *F thanks for giving me the possibility to clarify my assertions: *F \- duo is the mutant's name. It describes the cytological phenotype of a *F polyA-binding protein allele. *F \- by in situ ibridization we have positioned the P insertion at 55B. *F Thank you, *F with best wishes, *F Barbara *F Barbara Fasulo *F Dipartimento di Genetica e Biologia Molecolare *F Universita' di Roma 'La Sapienza' *F Ple Aldo Moro 5 *F Roma 00185 *F ITALY *F Tel: 39-6-49912556 *F Fax: 39-6-4456866 *F Email: bfasulo@axcasp.caspur.it # *U FBrf0111607 *a Voelkner *b M. *t 1999.9.15 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID256 *F e-mail: voelkner@mpih-frankfurt.mpg.de *F author: Volkner, M. *F Dear Dr. Volkner, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Cloning and characterization of two newly identified glutamate *F receptor genes in Drosophila' *F You mention 2 glutamate receptor genes that are new to FlyBase, *F 'DGluR-IB' and 'DKaiR-IA'. We do not prefix with 'D' for Drosophila in *F FlyBase, so the 'D' at the beginning of the symbol must be dropped. *F Also, the format of the gene symbols for these type of receptors in *F FlyBase is 'Glu-RA', 'Glu-RI', 'Glu-RIIA' etc. Would it be OK with you *F if the valid symbols for you 2 genes in FlyBase were 'Glu-RIB' and *F 'Kai-RIA' to fit in with this format ? *F Does the 'Kai' stand for 'Kainate' - if so, the full name for this gene *F in FlyBase could be 'Kainate receptor IA' ? *F Also, do you have a map location for 'DGluR-IB' and 'DKaiR-IA'? It is *F nice if we can keep as many gene records as possible anchored to the *F map. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Dear Chihiro, *F Thank you for your interest. In principle for me it would be OK naming them Glu- *F RIB and Kai-RIA. It is also right that the Kai stands for Kainate but this only *F comes from sequence-homologies to vertebrate-receptor-sequences. So it might be *F better naming it Glu-RIIIA if possible. The location for Glu-RIB is '3L 67 A,B' *F and for Kai-RIA '3R 90C,D'. *F Best regards, *F Michaela. *F Michaela Voelkner *F Max Planck-Institute for Brain Research *F Neurochemistry *F Deutschordenstrasse 46 *F 60528 Frankfurt/Main *F Germany *F Tel.: ++49-69-96769-226 *F Fax.: ++49-69-96769-447 *F email: voelkner@mpih-frankfurt.mpg.de # *U FBrf0111608 *a Adam *b G. *t 1999.9.15 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID250 *F e-mail: adamg@nucleus.szbk.u-szeged.hu *F author: Adam, G *F Dear Dr. Adam, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Drosophila importin-alpha2 protein is required for both the slow and *F the rapid phases of nurse cell cytoplasm transport during oogenesis' *F You mention a gene that is new to FlyBase, 'imp-alpha2' (Dimp-alpha2, *F we must drop the 'D' - we do not prefix with D for Drosophila in *F FlyBase). *F Do you have a map location for imp-alpha2? It is nice if we can *F keep as many gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Dear Dr. Yamada, *F The Dimp alpha2 actually is not a new gene, it is identical with *F oho31 and pendulin. We renamed it for two reason: 1. It shows the *F greatest homology with the importin alpha2 proteins. 2. Meantime *F two other importin homologes imp alpha 1 and 3 was also identified *F in Drosophila. *F Best wishes, *F Geza *F Institute of Genetics *F Biological Research Center *F Hungarian Academy of Sciences *F P.O.Box 521 *F H-6701 Szeged *F Hungary *F Tel:36 62 432 232 *F Fax:36 62 433 503 *F 36 62 432 576 *F E-mail: adamg@everx.szbk.u-szeged.hu # *U FBrf0111609 *a Gangadharan *b U. *t 1999.9.16 *T personal communication to FlyBase *u *F Dear Dr. Gangadharan, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Identification and characterisation of a Drosophila homologue to the *F Ablphilin gene family' *F You mention a gene that is new to FlyBase, Ablphilin. *F Do you have a short symbol for Ablphilin (Abl and Abi are already in *F use, but Ablp is not) ? *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Dear Dr Yamada, *F The Abelson interacting (Abi) proteins have been re-named Ablphilin in *F Mouse and human. As these genes were initially identified in vertebrates I *F have kept to the same name. *F I have also just recently (after I submitted my abstract)identified from *F Flybase that our work overlaps with work carried out by Juang et al. He *F has shortened the Drosophila Abelson interacting protein gene to dAbi. *F I hope this answers your query. *F with best wishes *F Uma Gangadharan *F Dear Chihiro, *F The Juang et al., paper has not been published yet and I have been unable *F to get a pre-print - however from what I have gleaned from the database our *F sequences are identical - enough to confirm that they are the same gene *F without looking at mapping data. *F Uma # *U FBrf0111610 *a Peter *b A. *t 1999.9.24 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID263 *F e-mail: apeter@gwdg.de *F author: Peter, A. *F Dear Dr. Peter, *F We are currently curating the abstracts for the upcoming European Drosophila Research Conference in Zurich, for FlyBase. I am writing in connection with your abstract: *F 'Characterization of a gene encoding the kinesin associated protein 3 *F (KAP3) in Drosophila melanogaster' *F You mention a gene that is new to FlyBase, KAP3. *F Do you have a map location for KAP3? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Dear Dr. Yamada, *F I am sorry for the delayed answer on your request: *F The kinesin-associated protein 3 (KAP3)-gene was mapped to chromosomal band *F 10B1-2 by in situ hybridization. I hope this information is sufficient for *F you. *F Thank you for your patience, *F Annette Peter *F Annette Peter *F Max Planck-Institut für biophysikalische Chemie *F Abteilung Molekulare Entwicklungsbiologie *F Am Fassberg 11 *F 37077 Göttingen *F Tel.: 0551-201-1 *F Fax: 0551-201-1755 *F e-mail: apeter@gwdg.de # *U FBrf0111611 *a Chen *b S. *t 1999.9.26 *T personal communication to FlyBase *u *F From: 'Sa Chen' *F To: cy200@gen.cam.ac.uk *F Subject: Re: EDRC99 - abstract ID105 *F Date: Sun, 26 Sep 1999 01:09:17 PDT *F Dear Dr.Chihiro: *F The number of the EST is LD33764, which is in the chromosomal region of *F 47D-E.But we are not sure yet if this EST is the MAO gene, because the *F deduced protein with 20% identity and 35% similarity to human MAO B. *F >From: Chihiro Yamada *F >To: chen_sa@hotmail.com *F >Subject: EDRC99 - abstract ID105 *F >Date: Tue, 14 Sep 1999 18:03:13 +0100 *F > *F >Subject: EDRC99 - abstract ID105 *F >e-mail: chen_sa@hotmail.com *F >author: Chen, S. *F > *F >Dear Dr. Chen, *F > *F >We are currently curating the abstracts for the upcoming European *F >Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F >connection with your abstract: *F > *F >'Search for a monoamine oxidase gene in Drosophila' *F > *F >You mention a gene that is new to FlyBase, MAO. *F > *F >You state that you found an EST clone for this gene - could you tell me *F >the identifier number of this EST, e.g. LD10356, GH78935. If we know *F >these things we can make the proper links in FlyBase. *F > *F >Thank you for your help, *F > *F >with best wishes, *F > *F >Chihiro *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >--------------------------------------------------------------------- # *U FBrf0111612 *a Graba *b Y. *t 1999.9.27 *T personal communication to FlyBase *u *F Subject: EDRC99 - abstract ID327 *F e-mail: graba@lgpd.univ-mrs.fr *F author: Graba, Y. *F Dear Dr. Graba, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Chameau, a novel Drosophila putative acetyl transferase, functions in *F Pc-G gene silencing and bristle specification' *F You mention a gene that is new to FlyBase, chameau. *F Do you have a map location for chameau? It is nice if we can keep as *F many gene records as possible anchored to the map. *F Also, do you have a short symbol for chameau, such as 'cham' ? *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F Date: Mon, 27 Sep 1999 08:00:06 +0100 *F To: Chihiro Yamada *F From: Yacine GRABA *F Subject: Re: EDRC99 - abstract ID327 *F Dear Chihiro, *F Sorry for the delay in replying and thanks for recalling me. *F The gene was mapped by in situ hybridisation to 28A. *F The short symmbol for chameau could be chm if not already used. If so *F please let us know. *F If there is anything alse please do not hesitate to be in touch *F Yacine *F Dr. Yacine GRABA, *F IBDM, *F LGPD, CNRS Case 907 *F Parc Scientifique de Luminy *F 13288 Marseille cedex 9 *F FRANCE *F e-mail: graba@lgpd.univ-mrs.fr # *U FBrf0111613 *a Van Daele *b E. *t 1999.9.27 *T personal communication to FlyBase *u *F To: Chihiro Yamada *F Subject: Re: EDRC99 - abstract ID304 *F Content-Transfer-Encoding: 7bit *F Dear Chichiro, *F Sorry for answering so late. I had first to consult with people we are *F collaborating with and they were on holiday during the previous weeks. *F The phenotypes I describe in the poster are rescued by expression of *F Ptpa (gene already in Flybase). Recombination & deficiency mapping *F agree with that. The chromosome was called J8 when isolated, so we call *F the allele PtpaJ8 now. *F Eddy *F Chihiro Yamada wrote: *F > *F > Dear Dr Van Daele, *F > *F > Just over a week ago, I sent you a query concerning your abstract for *F > the upcoming EDRC99 conference. I was wondering if you'd had a chance *F > to look at it. I enclose the mail below for your convenience. *F > *F > Thanks *F > *F > Chihiro *F > *F > > From cy200 Wed Sep 15 09:53:37 1999 *F > > To: paper@biols.susx.ac.uk *F > > Subject: EDRC99 - abstract ID304 *F > > X-Sun-Charset: US-ASCII *F > > Content-Length: 1126 *F > > *F > > Subject: EDRC99 - abstract ID304 *F > > e-mail: evandaele@biols.susx.ac.uk *F > > author: Van Daele, E. *F > > *F > > Dear Dr. Van Daele, *F > > *F > > We are currently curating the abstracts for the upcoming European *F > > Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F > > connection with your abstract: *F > > *F > > 'A gene necessary for multiple steps in bristle development' *F > > *F > > You describe a new mutation affecting sensory organ development. Do *F > > you have a name/symbol for this mutation yet ? We need a symbol in *F > > order to be able to record its existence for FlyBase. *F > > *F > > Also, do you have a map location for the gene? It is nice if we can *F > > keep as many gene records as possible anchored to the map. *F > > *F > > Thank you for your help, *F > > *F > > with best wishes, *F > > *F > > Chihiro *F > > ---------------------------------------------------------------------- *F > > Chihiro Yamada. *F > > *F > > FlyBase (Cambridge), *F > > Department of Genetics, *F > > University of Cambridge, *F > > Downing Street, email: c.yamada@gen.cam.ac.uk *F > > Cambridge, CB2 3EH, Ph : 01223-333963 *F > > UK. FAX: 01223-333992 *F > > ---------------------------------------------------------------------- *F > > *F \-- *F Eddy Van Daele *F School of Biological Sciences *F University of Sussex *F Brighton UK *F BN1 9QG *F Email evandaele@biols.susx.ac.uk *F Tel. +44 01273 678978 *F Fax +44 01273 678433 # *U FBrf0111614 *a Dickson *b B. *t 1999.9.23 *T personal communication to FlyBase *u *F Dear Barry, *F Thanks you for your very prompt reply (you were the first back). Your *F abstract will now become a reference for flamingo. *F Best Wishes *F Chihiro *F > Dear Chihiro, *F > *F > The cadherin is the gene Flamingo, recently published by Tadashi Uemura's *F > group in Cell. *F .. *F > *F > With best wishes, *F > Barry *F > *F > *F >Dear Dr Dickson, *F > *F >Just over a week ago, I sent you a query concerning your abstract for *F >the upcoming EDRC99 conference. I was wondering if you'd had a chance *F >to look at it. I enclose the mail below for your convenience. *F > *F >Thanks *F > *F >Chihiro *F > *F >> From cy200 Tue Sep 14 17:49:13 1999 *F >> To: dickson@nt.imp.univie.ac.at *F >> Subject: EDRC99 - abstract ID44 *F >> X-Sun-Charset: US-ASCII *F >> Content-Length: 1150 *F >> *F >> Subject: EDRC99 - abstract ID44 *F >> e-mail: dickson@nt.imp.univie.ac.at *F >> author: Dickson, B *F >> *F >> Dear Dr. Dickson, *F >> *F >> We are currently curating the abstracts for the upcoming European *F >> Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F >> connection with your abstract: *F >> *F >> 'Genes controlling photoreceptor axon guidance in Drosophila' *F >> *F >> You mention a gene that are new to FlyBase, encoding a *F >> 'serpentine cadherin' *F .. *F >> *F >> Do you have a symbol and/or names for this gene? *F >> *F >> Also, do you have a map location for it? It is nice if we can keep *F >> as many gene records as possible anchored to the map. *F >> *F >> Thank you for your help, *F >> *F >> with best wishes, *F >> *F >> Chihiro *F >> ---------------------------------------------------------------------- *F >> Chihiro Yamada. *F >> *F >> FlyBase (Cambridge), *F >> Department of Genetics, *F >> University of Cambridge, *F >> Downing Street, email: c.yamada@gen.cam.ac.uk *F >> Cambridge, CB2 3EH, Ph : 01223-333963 *F >> UK. FAX: 01223-333992 *F >> ---------------------------------------------------------------------- *F >> *F \-------------------------------------------------- *F Barry Dickson *F Research Institute of Molecular Pathology (I.M.P.) *F Dr. Bohr-Gasse 7 *F A-1030 Vienna *F Austria *F Tel. +43 1 797 30 421 *F Fax. +43 1 798 71 53 *F Email. dickson@nt.imp.univie.ac.at *F \-------------------------------------------------- # *U FBrf0111615 *a Steinmann-Zwicky *b M. *t 1999.9.28 *T personal communication to FlyBase *u *F From cy200 Wed Sep 15 09:59:14 1999 *F To: paser@zool.unizh.ch *F Subject: EDRC99 - abstract ID399 *F X-Sun-Charset: US-ASCII *F Content-Length: 1145 *F Subject: EDRC99 - abstract ID399 *F e-mail: paser@zool.unizh.ch *F author: Sergeev, P. *F Dear Dr. Sergeev, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'Molecular characterization of falten, a gene required for correct *F morphogenetic movements during gastrulation' *F You mention a gene that is new to FlyBase, fal. *F In your abstract you state that 'parts of fal overlap with pipe'. *F Could you confirm that this means that the transcription units overlap *F ? Also, are the genes transcribed in the same or opposite direction and *F which ends of the transcripts overlap ? *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From cy200 Wed Sep 15 09:58:47 1999 *F To: aheller@rzu-mailhost.unizh.ch *F Subject: EDRC99 - abstract ID398 *F X-Sun-Charset: US-ASCII *F Content-Length: 1038 *F Subject: EDRC99 - abstract ID398 *F e-mail: aheller@rzu-mailhost.unizh.ch *F author: Heller, A. *F Dear Dr. Heller, *F We are currently curating the abstracts for the upcoming European *F Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F connection with your abstract: *F 'falten, a gene that is required for correct morphogenetic movements *F during gastrulation, acts independently of the fog pathway' *F You mention a gene that is new to FlyBase, fal. *F Do you have a map location for fal? It is nice if we can keep as many *F gene records as possible anchored to the map. *F Thank you for your help, *F with best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From mstzw@zool.unizh.ch Tue Sep 28 09:51:23 1999 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Tue, 28 Sep 1999 09:51:23 +0100 *F X-Sender: mstzw@rzu-mailhost.unizh.ch *F Mime-Version: 1.0 *F Date: Tue, 28 Sep 1999 13:35:19 +0200 *F To: cy200@gen.cam.ac.uk *F From: mstzw@zool.unizh.ch (Monica Steinmann-Zwicky) *F Subject: Your question *F Dear Chihiro *F Both Pavel Sergeev and Astrid Heller gave me your e-mails concerning a gene *F we call fal in our abstracts. I am sorry I could not answer earlier. I am *F very much uptaken with the organisation of the EDRC99. Here my answers: map *F location 76A, yes the transcription units overlap. The genes fal and pipe *F are transcribed in opposite directions with overlapping 3'ends. I hope this *F helps. Best wishes, *F PD Dr. Monica Steinmann-Zwicky *F Zoological Institute, University of Zurich *F Winterthurerstrasse 190 *F CH-8057 Zurich, Switzerland *F Tel: ++41 1 635 48 72 *F Fax: ++41 1 635 68 23 # *U FBrf0111616 *a Davies *b J. *c K. *d Curtin *t 1999.9.30 *T personal communication to FlyBase *u *F From cy200 Tue Sep 28 12:33:23 1999 *F To: flycam *F Subject: Re: prp33/innexins *F X-Sun-Charset: US-ASCII *F Content-Length: 3342 *F \----- Begin Included Message ----- *F From bafu6@central.susx.ac.uk Tue Sep 28 11:13:39 1999 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Tue, 28 Sep 1999 11:13:39 +0100 *F X-Sender: bafu6@mailhost.central.sussex.ac.uk *F Mime-Version: 1.0 *F To: Chihiro Yamada *F Subject: Re: prp33/innexins *F Date: Tue, 28 Sep 1999 11:13:58 +0100 *F From: J.A.Davies@sussex.ac.uk (Jane Davies) *F Dear Chihiro, Kathy Curtin and I have put our heads together about the *F naming of the innexins and this is the schemethat we have come up with: *F Name Synonym Orthologue *F Ogre Dm-inx1 Sa-inx(1) *F Dm-inx2 prp33 Sa-inx(2) *F Dm-inx3 (Stebbings et al, submitted to J. Cell Biol.) *F Dm-inx6 prp6 *F Dm-inx7 prp7 *F I know this leaves us minus Dm-inx 4 & 5 but I imagine the gap will soon be *F filled. *F I have enclosed a copy of my correspondence with Kathy, but please get in *F touch with her if you require independent confirmation. *F Best Wishes *F Jane Davies *F Envelope-to: bafu6@central.susx.ac.uk *F X-Authentication-Warning: mercury.cis.yale.edu: kdc4 owned process doing -bs *F Date: Wed, 22 Sep 1999 13:13:04 -0400 (EDT) *F From: Kathy Curtin *F X-Sender: kdc4@mercury.cis.yale.edu *F To: Jane Davies *F Subject: Re: innexins *F MIME-Version: 1.0 *F Status: *F Jane: *F This sounds fine and good to me. Tell Flybase to go ahead. *F Thanks, *F Kathy *F On Wed, 22 Sep 1999, Jane Davies wrote: *F > >Dear Jane: *F > > *F > > I have no real objection to renaming prp33 since the name is *F > >somewhat meaningless. But we should also then rename the two other prp *F > >genes 6 and 7. Does that sound reasonable? Then if someone finds an *F > >orthlogue in another speices they can follow the Drosophila nomenclature. *F > >Though I wonder how easy it will be to idntify orthologues with certainty *F > >when many family members remain unidentified. But perhaps we have to name *F > >as we go. I will talk to Bob about the other prp genes and write to the *F > >fellow at Flybase. We did agree to the innexin name if somewhat *F > >reluctantly. *F > > *F > >Kathy *F > > *F > > *F > > *F > >Dear Kathy *F > *F > Thanks for your prompt reply. I think that the best thing is to re-name all *F > the 'prp' genes if you and Bob agree, but have 'prp' as a synonym. I *F > realise that it is often difficult to identify orthologues but, as you say, *F > the best thing is just to name as we go and fine-tune the system as *F > necessary. *F > *F > This is my suggested scheme: *F > *F > Name Synonym Orthologue *F > *F > Ogre Dm-inx1 Sa-inx(1) *F > Dm-inx2 prp33 Sa-inx(2) *F > Dm-inx3 (Stebbings et al, submitted to J. Cell Biol.) *F > Dm-inx6 prp6 *F > Dm-inx7 prp7 *F > *F > I know this leaves us minus Dm-inx 4 & 5 but I imagine the gap will soon be *F > filled. If you think this system is OK, I'll forward this email to Flybase *F > and to Jocelyn Shaw. *F > *F > Best Wishes *F > *F > Jane Davies *F > *F > *F > Dr Jane Davies *F > Sussex Centre for Neuroscience *F > Sussex University *F > Falmer *F > Brighton BN1 9QG *F > UK *F > *F > Tel: 01273-606-755 ex 2746 (office) *F > 01273-678-055 (secretary) *F > 01273-678-512 (lab) *F > Fax: 01273-678-535 *F > email: j.a.davies@sussex.ac.uk *F > *F > *F Dr Jane Davies *F Sussex Centre for Neuroscience *F Sussex University *F Falmer *F Brighton BN1 9QG *F UK *F Tel: 01273-606-755 ex 2746 (office) *F 01273-678-055 (secretary) *F 01273-678-512 (lab) *F Fax: 01273-678-535 *F email: j.a.davies@sussex.ac.uk *F \----- End Included Message ----- *F >Dear Jane and Kathryn, *F > *F >I'm glad to see that you've managed to come to an agreement. There is *F >one point however, FlyBase has a policy of assuming that all genes in *F >its database are Drosophila genes and thus drops any 'D' or 'Dm' *F >suffixes as being superfluous. So the gene names in FlyBase will be:- *F > *F > *F >Name Synonym Orthologue *F > *F >Ogre Dm-inx1 Sa-inx(1) *F >inx2 prp33 Sa-inx(2) *F >inx3 (Stebbings et al, submitted to J. Cell Biol.) *F >inx6 prp6 *F >inx7 prp7 *F > *F >Obviously when referring to these genes in the context of the whole *F >family of genes, the 'Dm' prefix is necessary, but FlyBase does not *F >consider it to be part of the gene name. *F > *F >I hope this doesn't cause any problems. *F > *F >Best wishes, *F > *F >Chihiro. *F > *F Dear Chihiro *F That's fine! *F Jane *F Dr Jane Davies *F Sussex Centre for Neuroscience *F Sussex University *F Falmer *F Brighton BN1 9QG *F UK *F Tel: 01273-606-755 ex 2746 (office) *F 01273-678-055 (secretary) *F 01273-678-512 (lab) *F Fax: 01273-678-535 *F email: j.a.davies@sussex.ac.uk # *U FBrf0111622 *a Schafer *b M.A. *t 1999.9.30 *T personal communication to FlyBase *u *F Archived. # *U FBrf0111623 *a Siekhaus *b D. *t 1999.9.22 *T personal communication to FlyBase *u *F From siekhaus@uclink4.berkeley.edu Wed Sep 22 19:38:28 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 22 Sep 1999 19:38:28 +0100 *F Mime-Version: 1.0 *F X-mailer: Eudora Pro 3.0 for Macintosh *F Date: Tue, 21 Sep 1999 17:53:10 -0700 *F To: flybase-help@morgan.harvard.edu *F From: Daria Siekhaus *F Subject: update to info on amontillado *F Content-Type: multipart/mixed; boundary='=_AAArYQAALqg36esw' *F Content-Length: 1914 *F Dear Flybasers, *F Under the info for the gene amontillado or dPC2 which I cloned, you *F state that there are 2 references but you only list the one from the *F fly meeting, and not the recent paper that has finally emerged: *F Daria E.Siekhaus and Robert S. Fuller (1999). A Role for *F amontillado, the Drosophila Homolog *F of the Neuropeptide Precursor Processing Protease PC2, in Triggering *F Hatching Behavior. J. Neurosci. 19:6942-6954. *F I think the gene should also be renamed amontillado, *F with amon as its symbol, and PC2 and dPC2 put as *F synonyms. The full gene sequence is available at GenBank, Accession \# *F AF033117. *F There are also 3 ESTs which corresponds to the sequence: *F GH12584.5', GH22014.5' and HL03794.5' which are currently listed as *F corresponding to no known sequence. *F Under other info you might want to include the details on expression *F and link to behavior listed in the abstract for the paper above. *F Many thanks for all your great work!! *F Daria Siekhaus *F From gm119@gen.cam.ac.uk Thu Sep 23 09:28:04 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 23 Sep 1999 09:28:04 +0100 *F To: siekhaus@uclink4.BERKELEY.edu *F Subject: Re: update to info on amontillado *F Cc: flybase-help@morgan.harvard.edu *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 23 Sep 1999 09:29:26 +0100 *F Content-Length: 747 *F Dear Daria, *F further to what Aubrey wrote in reply to your e-mail about amontillado, *F I notice that in your e-mail you state that the 3 ESTs: *F GH12584.5', GH22014.5' and HL03794.5' *F correspond to amontillado. *F Since we do not have this information in FlyBase, I will record the *F above information as a personal communication from you to FlyBase. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0111624 *a McHugh *b B. *t 1999.10.4 *T personal communication to FlyBase *u *F Archived. # *U FBrf0111625 *a Thomas *b G. *c B. *d MacIver *t 1999.8.19 *T personal communication to FlyBase *u *F >From rd120@gen.cam.ac.uk Wed Aug 04 12:45:38 1999 *F To: gxt5@psu.edu *F Subject: Helping FlyBase: kst01318 *F Dear Graham, *F You may be able to help FlyBase out. In your Development paper: *F FBrf0102732 == Thomas et al., 1998, Development 125(11): 2125--2134 *F you mention revertants of kst01318 (P-element excisions), and say *F that their eye phenotype is reverted. Is the reduced viability seen *F for kst01318 restored to normal? The reason I ask is that Thuy *F Nguyen told us that the 'lethality' of the chromosome is not associated *F with the P{PZ}, but this may have been a misunderstanding about what *F we were asking. *F We need to know because it has an effect on how many mutations we *F associate with the 01318 chromosome. *F We'd be grateful for any help you can give us. *F Regards, *F Rachel. *F >From gxt5@psu.edu Wed Aug 04 14:46:44 1999 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 4 Aug 1999 14:46:44 +0100 *F X-Sender: gxt5@mail.psu.edu *F Mime-Version: 1.0 *F Date: Wed, 4 Aug 1999 09:48:31 -0400 *F To: Rachel Drysdale (Genetics) *F From: Graham Thomas *F Subject: Re: Helping FlyBase: kst01318 *F Dear Rachel, *F What she means is that the chromosome is homozygous lethal due to the *F presence of an ancillary lethal not associated with the P-element in karst. *F The karst allele associated with the P-element is actually unusually viable *F in combination with most other karst alleles and Df near 63. The lethal to *F which Thuy refers is somewhere in the 70's and is easily recombined away *F from the kst mutation. We roughly mapped this some time back with the Df *F kit I think. However, the postdoc who did this, and could tell you *F precisely which Df uncovers the lethal, is away on holiday right now, but I *F will copy him in on this response and he will fill you in. In the meantime *F don't put anything in the database based on the above just yet.... *F What brought this up? *F Cheers! *F Graham *F >From rd120@gen.cam.ac.uk Wed Aug 04 15:07:33 1999 *F To: gxt5@psu.edu *F Subject: Re: Helping FlyBase: kst01318 *F Dear Graham, *F When I hear from the post doc I will curate his and your *F mail as a personal communication from you and him to us, to get in the *F data that kst01318 is itself viable and was originally on the same *F chromosome as the l(3)... hit. If you could pass on this message to *F him that would be great. *F The reason that this came up was that one of the FlyBase folk (Kathy *F Matthews) noticed that the kst01318 record said both 'semi-lethal' *F and 'The lethality of the @kst01318@ chromosome is not associated *F with a closely linked @P{PZ}@ insertion.', and smelled a rat *F (kst01318 is in her Stock Center). *F best wishes, *F Rachel. *F >From ibm2@psu.edu Thu Aug 19 16:10:26 1999 *F To: rd120@gen.cam.ac.uk *F Subject: kst01318 and lethal locus *F Dear Rachel, *F With respect to the lethal associated with the kst01318 allele, I mapped *F the lethal to Df (3L) 2993 which, according to Flybase, uncovers *F 72C01-D01;73A03-04. Hope this is what you need. *F best regards *F Bryce MacIver # *U FBrf0111626 *a MacIver *b B. *t 1999.8.19 *T personal communication to FlyBase *u *F From ibm2@psu.edu Thu Aug 19 16:10:26 1999 *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F It is time I told someone *F that a gene I cloned while in Edinburgh is not where we thought it was. The *F gene is given the name NaPi-T in Flybase and is incorrectly mapped to the *F 43C region. Based on sequence homology it is a putative sodium dependent *F phosphate co-transporter, although some part time collaboration with a *F friend of mine failed to conclusively demonstrate that function in frog *F oocytes (meaning we need to address the problem on more than a part time *F basis). I was trying an in situ hybridisation to polytene chromosomes with *F cDNA before I left Edinburgh but could not get a result. However, the *F genomic DNA covering the region gave multiple locations most of which were *F on the third chromosome. So, it wouldn't surprise me if this one ends up *F being on the 3rd. We originally thought it was contiguous with the myosin V *F (didum) gene we cloned but it appears some odd cross hybridisation was *F going on during our screening and cloning. *F best regards *F Bryce MacIver # *U FBrf0111627 *a Shilo *b B. *t 1999.8.22 *T personal communication to FlyBase *u *F From rd120 Thu Aug 12 16:15:38 1999 *F To: lvshilo@weizmann.weizmann.ac.il *F Subject: Helping FlyBase: UAS-Egfr constructs *F Dear Dr. Shilo, *F You may be able to help me out about some wild type Egfr UAS *F constructs. The two papers this concerns are: *F \*x FBrf0102828 == Roch et al., 1998, Development 125(10): 1823--1832 *F and *F \*x FBrf0099737 == Wappner et al., 1997, Development 124(22): 4707--4716 *F The Materials and Methods of Roch et al. thank you for a wild type *F UAS-DER construct. *F Your own Wappner et al., 1997 mentions, again in Materials and Methods, *F a UAS-DER typeII, with no details about its construction. *F Is this UAS-DER the same as the one described in *F \*x FBrf0102731 == Buff et al., 1998, Development 125(11): 2075--2086 *F ? *F This is the one made in the Michelson lab and used by the Michelson and *F Freeman labs. *F If the one you used is a different one, made in your lab, please can *F you tell me a little about it (who made it, presumably it is on pUAST, *F but which bit of Egfr is it) so I can make an accurate record of it for *F our files. *F Many thanks for your help, *F Rachel. *F From lvshilo@weizmann.weizmann.ac.il Sun Aug 22 06:48:10 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: UAS-Egfr constructs *F Dear Rachel, *F The UAS-DER II construct we used was made by Myriam Golembo and *F Erez Raz in my lab. It contains the full length DER type II cDNA in pUAST. *F . *F . *F The type I and II DER cDNAs *F differ only in their 5' exon which encodes the signal peptide and a few *F residues following it. *F I hope this helps. *F Best wishes, *F Benny Shilo # *U FBrf0111628 *a Gergen *b P. *t 1999.8.23 *T personal communication to FlyBase *u *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Peter Gergen, SUNY *F To: Bloomington Drosophila Stock Center *F Subject: Scer\GAL4[nos.PG] *F Date: 23 August 1999 *F *F Background: additional information on Scer\GAL4[nos.PG], carried by P{GAL4-nos.NGT} *F *F Information communicated: *F The 3' UTR used in the Scer\GAL4[nos.PG] allele was derived from alphaTub84B. # *U FBrf0111629 *a Tully *b T. *t 1999.7.2 *T personal communication to FlyBase *u *F From tully@cshl.org Fri Jul 02 00:00:37 1999 *F To: flybase-help@morgan.harvard.edu *F Hi Rachel (?): *F FlyBase persists with a confusion between linotte and derailed, which *F should be corrected. Below is an attempt to clarify the issues, which I *F also have sent to Interactive Fly. *F . *F . *F . *F Here's the linotte story as we see it: *F 1. The linotte transcript is novel (i.e., not derailed), and hs-induced *F expression of a lio+ transgene in adults is sufficient to rescue fully the *F adult learning defect of lio1 mutants (Bolwig et al.,1995). *F 2. The original lio1 P element mutation (as described by Dura et al., *F 1993) is inserted 800 bp downstream of the linotte transcription unit, *F perhaps in the 5'UTR of derailed. In fact, a P element allele of derailed, *F isolated by John Thomas' lab, is inserted 6 bp away from our lio1 allele. *F Hence, one might expect the lio1 mutation to create some sort of derailed *F phenotype. *F 3. Dura's group has generated an excision-deletion of derailed by *F mobilizing lio1. They call this deletion allele, lio2. They have shown *F that (i) lio2 molecularly deletes derailed but not linotte and (ii) the *F anatomical defects of lio2 are more severe than those of lio1. *F 4. Most mutant analyses by Dura's group, whether they be anatomical or *F especially molecular (i.e. rescue), have been done on lio2, thereby *F complicating everything. *F 5. Lio2 produces a more severe learning defect than lio1. Since the *F anatomical defect of lio2 also is more severe than lio2, I assume that the *F lower learning score in lio2 is caused not by a mechanistic effect on *F learning but rather by and additional effect on the anatomy of mushroom *F bodies (and who knows were else). *F Hope this helps. *F Cheers, *F Tim # *U FBrf0111630 *a Mann *b R.S. *t 1999.8.31 *T personal communication to FlyBase *u *F From rsm10@columbia.edu Tue Aug 31 16:41:35 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: correction *F I would like to correct an error in the map position of the gene nervy, as *F described in a paper published in Genetics by my lab in 1995 (Feinstein, P., K. *F Kornfeld, D. Hogness, and R. S. Mann. (1995) Identification of Homeotic Target *F Genes in Drosophila melanogaster Including nervy, a Proto-Oncogene Homologue. *F Genetics 140, 573-586.). In that paper, nervy was mapped to 99F. However, we *F recently discovered that our original polytene in situ data mapped the gene to *F 60C. We believe that the 99F position was an error introduced during the writing *F of the paper. The 60C position for nervy was recently brought to my attention *F by Francois Karch, who was doing some sequencing in the 60C region, and has *F since been confirmed in my lab. *F We regret this error, and hope that Flybase will update its maps accordingly. *F Please don't hesitate to contact me if you need any clarification. For your *F information, I also notified the journal Genetics about this error, in case they *F would like to publish a correction. *F Sincerely, *F Richard S. Mann # *U FBrf0111631 *a Winberg *b M. *t 1999.9.3 *T personal communication to FlyBase *u *F From mwinberg@uclink4.berkeley.edu Fri Sep 03 20:20:57 1999 *F To: flybase-help@morgan.harvard.edu *F Subject: plexin synonym *F Hi there! *F Just thought I'd mention that the 'plex' gene reported by *F Locke et al (Genome Research, Feb 1999) is the same as *F the Plexin B gene in 101F. Maybe you could consolidate *F those files/pages? *F Thanks! *F Meg Winberg # *U FBrf0111632 *a Winberg *b M. *t 1999.9.4 *T personal communication to FlyBase *u *F >From mwinberg@uclink4.berkeley.edu Sat Sep 04 04:04:29 1999 *F To: Aubrey de Grey *F Subject: Re: plexin synonym *F The semaphorin-like gene identified by Yu et al (Genome *F Res 1997) was later named Sema1b? *F Best, *F Meg # *U FBrf0111633 *a Wassarman *b D. *t 1999.8.24 *T personal communication to FlyBase *u *F To: kaufman@bio.indiana.edu (T. Kaufman) *F Date: Tue, 24 Aug 1999 18:03:09 -0400 *F From: 'David A. Wassarman' *F Subject: Re: cell lethal mutants *F Dear Dr. Kaufman, *F By complementation analysis I have determined that cell lethal is allelic *F to TAF250. The cell1 allele (obtained from Jim Kennison) fails to *F complement all of my TAF250 alleles. Therefore, I would like to get your *F permission to change the name of cell lethal to TAF250 (i.e. cell1 would *F be TAF250cell-1). *F Sincerely, *F David # *U FBrf0111635 *a McKearin *b D. *t 1999.9.14 *T personal communication to FlyBase *u *F From bdgp-owner@fruitfly.bdgp.berkeley.edu Tue Sep 14 21:10:00 1999 *F From: dennis mckearin *F Subject: bdgp allelic designation of an EP line *F To: bdgp@fruitfly.bdgp.berkeley.edu *F FYI: *F line EP(3)0667 is inserted into the bag-of-marbles gene and acts as a *F strong bam allele. *F Dennis McKearin # *U FBrf0111636 *a Dow *b J. *t 1999.2.18 *T personal communication to FlyBase *u *F >From: Julian Dow *F >Date: Thu, 18 Feb 1999 07:12:45 -0800 *F >To: abeaton@uclink4.berkeley.edu *F >Subject: EP line request *F > *F . *F . *F >EP(3)3577, from the flanking DNA is an insertion in vha13, a plasma membrane *F >subunit of the vacuolar V-Type ATPase. *F > *F >With best wishes, Julian Dow # *U FBrf0111637 *a Ren *b X. *t 1999.9.17 *T personal communication to FlyBase *u *F From xren@nature.berkeley.edu Fri Sep 17 00:57:50 1999 *F Date: Thu, 16 Sep 1999 16:59:13 -0700 *F To: flybase-updates@morgan.harvard.edu *F Subject: New mutant name *F Hi, *F My name is Xiaoyun Ren from Mark Tanouye's lab at University of *F California-Berkeley. We would like to register a name for a new mutant: *F Phenotype: Bang-sensitive *F Location: Chromosome 2R-59A *F Name: jitterbug *F Abbreviation:jbug *F If you need more information please contact me. *F thanks *F \-una # *U FBrf0111638 *a Sekelsky *b J. *t 1999.9.21 *T personal communication to FlyBase *u *F From sekelsky@email.unc.edu Tue Sep 21 16:15:29 1999 *F Delivery-date: Tue, 21 Sep 1999 16:15:29 +0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: Ercc1 and mus210 *F I recently submitted a Genbank entry, AF146797, for the Drosophila gene *F encoding the homolog of the human nucleotide excision repair protein Ercc1. *F I had concluded that this protein is the product of the mus210 gene on the *F basis of: *F 1) The Ercc1 coding region maps to 51D1-2 by probing the P1 blot. *F 2) Hypersensitivity of mus210 mutants is uncovered by Df(2R)Jp1 (published *F breakpoints 51C3; 52F8-9 and Df(2R)XTE-18 (51D3-E1;52D1), but not by *F Df(2R)Jp4 (51F13;52F8-9). *F 3) The mus210B1 chromosome has a single nucleotide change (relative to *F other wild-type chromosomes) that changes a conserved Thr to Ala. *F I have since discovered that mus210 does not correspond to Ercc1. I was *F able to obtain a stock of an unrelated mus mutation that came out of the *F same screen as mus210B1, and discovered that the Ercc1 alteration in *F mus210B1 is a pre-existing polymorphism. I also learned that Df(2R)Jp1 *F and Df(2R)XTE-18 do not delete Ercc1. Df(2R)knSA3, however, does delete *F Ercc1, but does not uncover mus210. Complementation crosses with lethal P *F element inserts mapped by Todd Laverty suggests revisions for these Df *F breakpoints: *F Df(2R)knSA3 uncovers l(2)k08015 (51D3-5) *F Df(2R)knSA3 uncovers l(2)k06403 (51D7-8) *F Df(2R)knSA3 does not uncover boc (listed as 51D, but on a sequenced contig *F it is distal to chn, which is in 51E2) *F Thus, the distal breakpoint for Df(2R)knSA3 is probably 51D7-E2. *F Df(2R)Jp1 does not uncover l(2)k08015 (51D3-5) *F Df(2R)Jp1 uncovers l(2)k06403 (51D7-8) *F Thus, the proximal breakpoint for Df(2R)Jp1 is probably 51D3-8. *F Ercc1 maps to 51D1-2, is uncovered by Df(2R)knSA3, but not by Df(2R)Jp1 or *F Df(2R)XTE-18. There are no known mutations in this gene. I've sent an *F update request to Genbank to have them change the name associated with *F AF146797 from mus210 to Ercc1. *F So what is mus210? I've found that it corresponds to Xpcc, which encodes *F the nucleotide excision repair protein XPC, and which maps to 51F. The two *F published alleles are mus210B1 and mus210G1, formerly mus(2)201G1 or *F rad202G1. This latter allele is incorrectly listed in Flybase as an *F allele of mus201 (FBal0012621). Both alleles are nonsense mutations in *F Xpcc, and I've recovered two additional alleles (C1 and C2), which are also *F nonsense mutations (manuscript in prep). *F Jeff Sekelsky *F From rd120@gen.cam.ac.uk Wed Sep 22 14:55:24 1999 *F To: sekelsky@email.unc.edu *F Subject: Re: Ercc1 and mus210 *F >1) The Ercc1 coding region maps to 51D1-2 by probing the P1 blot. *F A ha - Jeff if you can tell me which particular P1 clone Ercc1 maps to *F I can usefully capture that info for FlyBase, too. *F Thanks very much, *F Rachel. *F From sekelsky@email.unc.edu Wed Sep 22 16:34:38 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Ercc1 and mus210 *F Hi Rachel. Ercc1 hybridizes to: *F DS09161 (51D1-2 by in situ) *F DS06422 (positive for Dm0765 from DS09161) *F DS06145 (positive for Dm0764 from DS09161; this STS was not used in contig *F building because of some ambiguous results, but clearly this P1 belongs on *F this contig). *F It does not hybridize to DS00158, which is also positive for Dm0765 and *F which has been placed in 51C1-D2 by in situ. *F So, Ercc1 is in the region of overlap between 09161, 06422, and 06145. # *U FBrf0111639 *a Klaembt *b C. *t 1999.9.23 *T personal communication to FlyBase *u *F From klaembt@uni-muenster.de Thu Sep 23 10:06:11 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Gene symbols *F Hi Rachel. *F while preparing some slides for a talk I noted a mistake in the genetic *F location of the gene schmalspur. In table 2 in Hummel et al., 1999 *F (Developmental Biology) we had placed schmalspur in 3-40. *F The correct location however is 3-2 cytologically it maps to 62A-B. *F Could you please change the mapping info in the Flybase schmalspur entry. *F many thanks for your help, *F christian # *U FBrf0111640 *a Langehans *b D. *t 1999.9.15 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Dirk Langehans, student in the laboratory of Christian Klaembt, Universitat Muenster. *F To: Bloomington Drosophila Stock Center *F Subject: Df(3L)Brd20 breakpoints *F Date: 15 September 1999 *F *F Background: Cytology on an aberration in the collection for which no cytology had been previously reported. *F *F Information communicated: *F *F The breakpoints of Df(3L)Brd20 have been cytologically mapped to 70D2-3;71E3-5. # *U FBrf0111641 *a Baumgartner *b S. *t 1999.9.23 *T personal communication to FlyBase *u *F From: Stefan Baumgartner *F To: EST@fruitfly.bdgp.berkeley.edu *F Subject: cdna GM10525 *F Hi, *F We just noticed that GM10525 can be assigned to the perlecan gene. We *F have cloned a portion of its cDNA independently, and it partially *F overlaps with some of our sequence. *F . *F . *F . *F Best wishes *F Stefan *F \-- *F From rd120@gen.cam.ac.uk Thu Sep 23 13:11:55 1999 *F To: Stefan.Baumgartner@medkem.lu.se *F Subject: Re: cdna GM10525 *F Hi Stefan, *F I saw your mail to the BDGP about GM10525. The correspondence between *F GM10525 and the perlecan gene is something I would like to capture for *F the FlyBase genes file, though we do not yet have a gene record for *F perlecan. Would it be OK for me to use your mail as a pc to FlyBase to *F serve as a source for the information? If so, do you have a symbol for *F perlecan? If not, I will simply use perlecan in full until you publish *F an abbreviated form. *F Many thanks. *F Best regards, *F Rachel. *F From Stefan.Baumgartner@medkem.lu.se Thu Sep 23 14:33:40 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: cdna GM10525 *F Hi Rachel, *F you can quote me; the gene name is somewhat difficult, all reasonable *F 3-letter names are already taken, except for 'pcn'. If 4 letters are *F allowed, then I would prefer 'pcan'. *F perlecan maps at 3A. You can also quote me on that, too. *F . *F . *F . *F Best wishes *F Stefan # *U FBrf0111642 *a Adler *b P. *t 1999.9.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Sep 22 20:49:17 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: koj and cant alleles *F *F The following information comes from correspondence with Paul Adler, *F University of Virginia, 9/22/99. *F *F koj[VAI51] and cant[WO21] were induced by EMS on FRT-bearing chromosomes. *F The cant mutation is homozygous lethal. The koj mutation is not lethal, *F but homozygotes are quite weak. *F *F koj maps to 47E and is allelic to shavenoid. shavenoid is a very *F misleading name since it implies similarity to shaven. In actuality, *F their phenotypes are completely different. shaven results in a lack of *F sensory bristles, but has no phenotype in trichomes while shavenoid has *F no phenotype in bristles but results in a dramatic loss of denticles *F and trichomes. # *U FBrf0111643 *a Bloomington Drosophila Stock Center *b ?. *t 1999.9.23 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: p[Xp] *F Date: 23 September 1999 *F *F Background: This allele is in the Bloomington collection but not in FlyBase. *F *F Information communicated: *F p[Xp], X-ray induced on TM6B-Tb *F phenotype is like p[p] *F generated in the Kaufman laboratory, Indiana University, sometime in the late 1970's or early 1980's # *U FBrf0111644 *a Bilder *b D. *t 1999.9.28 *T personal communication to FlyBase *u *F From bilder@rascal.med.harvard.edu Tue Sep 28 15:48:45 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: ADRC-331C *F Hi Rachel- *F We're getting ready to submit our paper on scrib next week so I *F thought I would ask you if you'd like to put the map location on Flybase. *F It's at 97B, and is in fact allelic to l(3)673 and l(3)j7B3. *F Thanks! *F D # *U FBrf0111645 *a Spana *b E. *t 1999.9.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Sep 27 20:20:07 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-EGFP} and P{UAS-srcEGFP} constructs and insertions from *F Eric Spana *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: P{UAS-EGFP} and P{UAS-srcEGFP} constructs and insertions from *F Eric Spana *F Eric Spana and Martin Zeidler, Harvard University have developed *F P{UAS-EGFP} and P{UAS-srcEGFP} lines. The following information from Eric *F Spana accompanied the stocks (September 1999). *F EGFP is a version of GFP from Clonetech that has two brightness/folding *F enhancing mutations and has been optimized for human codon usage. *F P{UAS-EGFP} constructs contain the Clonetech EGFP in the P{UAST} vector *F with the Drosophila translation initiation sequence substituted for the *F human initiation sequence upstream of the initiation codon. Insertions of *F P{UAS-EGFP} include: *F P{w+mC=UAS-EGFP}8 chromosome 1 localization unknown *F P{w+mC=UAS-EGFP}5a.2 chromosome 2 29C *F P{w+mC=UAS-EGFP}34 chromosome 3 86E13-18 *F Localization of P{UAS-EGFP}5a.2 and P{UAS-EGFP}34 was by P1 grid. *F The P{UAS-EGFP} constructs and insertions were made by Martin Zeidler *F P{w+mC=UAS-srcEGFP} constructs contain the 14 amino acid myristylation *F domain of v-src fused to the Clonetech EGFP with the consensus Drosophila *F translation start site in P{UAST}. Insertions include: *F P{w+mC=UAS-srcEGFP}M7A chromosome1 localization unknown *F P{w+mC=UAS-srcEGFP}M7E chromosome2 localization unknown *F The P{UAS-srcEGFP} constructs and insertions were made by Eric Spana. *F All insertions are homozygous viable and fertile. # *U FBrf0111648 *a Bloomington Drosophila Stock Center *b ?. *t 1999.8.10 *T personal communication to FlyBase *u *F >Date: Tue, 10 Aug 1999 18:23:59 -0500 *F >To: curators@morgan.harvard.edu *F >From: Kathy Matthews *F >Subject: upshot of Francis-Lang pc *F > *F >I'm concluding that *F > *F >l(3)j6E3 = P{lacW}sqdj6E3, l(3)j6E3j6E3 *F > *F >and reflecting this in the stock genotype. Let me know if I've missed *F >something here. *F > *F >-- K # *U FBrf0111649 *a Sekelsky *b J. *t 1999.10.10 *T personal communication to FlyBase *u *F From sekelsky@email.unc.edu Sun Oct 10 20:05:05 1999 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Sun, 10 Oct 1999 20:05:05 +0100 *F X-Sender: newtsky@imap-ns.med.unc.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.2.0.58 *F Date: Sun, 10 Oct 1999 15:06:50 -0400 *F To: flybase-updates@morgan.harvard.edu *F From: Jeff Sekelsky *F Subject: exciting data *F Mime-Version: 1.0 *F Dear Flybasers, *F ry506 is listed as a 3.4 kb deletion between +1.1 to +5.0. I sequenced a *F PCR product that spans this junction and determined that the deletion *F begins at +1397 (GTGCATTG...) and proceeds through +4885 (...TTATGAAAC). *F There is an insertion of a single A between the breakpoints (unless this A *F is a polymorphism on the ry+5 chromosome at +4885; I obtained the *F sequence between rosy and snake from Rob DeLotto, and I don't know the *F origin of his sequence). Enjoy. *F Jeff Sekelsky # *U FBrf0111650 *a Carpenter *b A.T.C. *t 1999.10.11 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Sat Oct 09 19:39:29 1999 *F To: rd120@gen.cam.ac.uk *F Subject: pc from Adelaide *F Information communicated: *F Df(3L)D-5rv14 is Df 70C14-15; 71C3-E5 plus In 71C3-E5; 72D3-12 *F Cheers A # *U FBrf0111651 *a BDGP Project Members *b ?. *t 1999.8.11 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in Arp53D *F Date: 11 Aug 1998 *F *F Request from FlyBase Curator: *F *F The alignment of Arp53D against the sequence in AC004341 shows a *F frameshift in the Berkeley sequence relative to the sequenced cDNA in *F GenBank (X78487). The G at 33174 of AC004341 is absent in X78487 and *F there is an A in X78487 between bases 33189 and 33190 of AC004341. That *F creates something like a 5 amino acid frameshift. Can you check *F this in the Berkeley sequence to see if perhaps there is a *F mistake? *F *F Response: *F *F I checked our sequence for the changes identified in the sequence of *F the cDNA. We have 3 subclones and 10 reads through the region, five *F forward reads and five reverse reads. All confirm the presence of the *F G and five As. I would assume the sequence for the cDNA is not as high *F quality. *F # *U FBrf0111652 *a BDGP Project Members *b ?. *t 1999.1.12 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in Ssb-c31a *F Date: 12 Jan 1999 *F *F Request from FlyBase Curator: *F *F In Ssb-c31a, a missing (?) base in the BDGP sequence leads *F to a CDS that is 13aa shorter than predicted in U18971). *F *F AC004532 20447 cggcaagaaggg (g?) catctccctat *F *F Response: *F *F This sequence is from one P1, one subclone 3 forward reads and 2 *F reverse reads all confirm the call only 3 G's. *F # *U FBrf0111653 *a BDGP Project Members *b ?. *t 1999.1.12 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in Pkc53E *F Date: 12 Jan 1999 *F *F Request from FlyBase Curator: *F *F In Pkc53E, an additional (?) base in the BDGP sequence *F leads to a change in the last 15aa of the protein and an extension of the *F protein by 31aa. *F *F AC004641 13921 aaagttggacacat (c?) cttgcggtgttt *F *F Response: *F *F Even though the accession is a build of 3 P1s this sequence is from one P1. *F Eight forward reads from one subclone and 9 reverse reads from 3 subclones *F all call 2 C's. *F # *U FBrf0111654 *a BDGP Project Members *b ?. *t 1999.9.8 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in Pcl *F Date: 8 Sep 1999 *F *F Request from FlyBase Curator: *F *F There is an extra base in the BDGP sequence relative to accession *F L35153 that leads to an extension of the Pcl CDS by almost 200 amino *F acids. Here's the sequence. The Accession is AC004296. *F *F tgtgatctatcatccga c? gagaattcaagcagcag *F *F Response: *F *F The c in our sequence is confirmed by 11 forward reads and 7 reverse reads. *F The region is spanned by two P1s so I am pretty confident that our sequence *F accurately reflects the genome. *F # *U FBrf0111655 *a BDGP Project Members *b ?. *t 1999.10.6 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in bsh *F Date: 6 Oct 1999 *F *F Request from FlyBase Curator: *F *F In bsh, there is a one base addition in the BDGP sequence relative to *F Acc. L06475 that leads to a 62aa extension of a CDS. Here's the *F surrounding sequence. *F *F AGCACAAAAAGCAGCT G(?) CGCCGGCGCGACAATG *F *F Response: *F *F The G is definitely there. As you can see the region is high GC and they *F probably had difficulty reading through the region with standard chemistry. *F The big dyes make a huge difference in our ability to get through these *F regions. I have 3 traces in the forward direction from two subclones and *F two traces in the reverse, all confirming the G. *F # *U FBrf0111656 *a BDGP Project Members *b ?. *t 1999.9.23 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in l(3)mbn *F Date: 23 Sep 1998 *F *F Request from FlyBase Curator: *F *F There are a number of sequence differences between the published *F sequence of l(3)mbn (Z47722) and the BDGP sequence. The first frame *F differences leads to a very early termination of the reading frame. In *F all there are three diffs that lead to frameshifts. The other *F differences involve the presence of 159 contiguous bases present in *F Z47722 but missing in AC004767 and 63 bases present in AC004767 but *F missing in Z47722. Both of those involve the presence or absence of *F repeat sequences and could be real strain differences. Their are a lot *F of repeats in the coding sequence. Here are some specifics. *F *F extra/missing bases *F *F 188138 tccgacttgg (g?) cgaaatacac (missing base between 188147 and 188148?) *F *F 188286 ggggtgtgtt (t?) cattctaagg (extra base at 188296?) *F *F 188607 tgctcctgtg (c?) accttgaccc (extra base at 188616?) *F *F *F insertions/deletions *F *F 191232 tccagttcaa (159 bases) ccaggtaagc (missing 159 bases between 191241 and *F 191242?) *F *F 191829 gggccttaa (63 bases) aaacattaat (extra 63 bases between 191837 and *F 191901) *F *F Response: *F *F I did investigate the sequence discrepancies and here is a summary *F report. I think we can explain the polymorphisms based on different *F strains. *F *F >extra/missing bases *F > *F >188138 tccgacttgg (g?) cgaaatacac (missing base between 188147 and 188148?) *F 5 forward, 5 reverse reads 9 subclones. All confirm no g *F *F >188286 ggggtgtgtt (t?) cattctaagg (extra base at 188296?) *F 4 forward, 4 reverse reads 4 subclones. All confirm extra t *F *F >188607 tgctcctgtg (c?) accttgaccc (extra base at 188616?) *F 6 forward 3 reverse. 6 subclones all confirm the c. *F *F *F >insertions/deletions *F > *F >191232 tccagttcaa (159 bases) ccaggtaagc (missing 159 bases between *F >191241 and 191242?) *F 6 forward 2 reverse. 4 subclones all confirm the deletion *F *F >191829 gggccttaa (63 bases) aaacattaat (extra 63 bases between 191837 and *F >191901) *F # *U FBrf0111657 *a BDGP Project Members *b ?. *t 1999.9.23 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Susan Celniker *F Co-Director Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in LanA *F Date: 23 Sep 1998 *F *F Request from FlyBase Curator: *F *F There's a base missing (?) that causes the LanA CDS to be terminated *F ~3/4 of the way into the coding region (it's only ~2600aa long rather *F than 3712aa!) *F *F The spot is in AC004767. Is there a base between 111131 and 111132? *F *F 111122 tcgagaactt (g?) aaggcccaag 111141 *F *F Response: *F *F I checked this discrepancy. 5 forward and 5 reverse reads confirm the *F sequence, from 7 subclones. Again it is in a region covered by only one *F P1. *F # *U FBrf0111658 *a Berkeley Drosophila Genome Project *b ?. *t 1999.10.27 *T personal communication to FlyBase *u *F Personal communication to FlyBase *F Received from: Berkeley Drosophila Genome Project *F Sequencing Center *F Subject: DNA Sequence check in klu *F Date: 27 Oct 1999 *F *F Request from FlyBase Curator: *F *F There is a one base addition in the BDGP sequence (clone D459, Acc. AC006562) *F relative to an accession (Y11066) that leads to a 53aa extension of a CDS. *F Here's the surrounding sequence. Please check. *F *F GAGGGCAAGGAGAGC C(?) GCGATTCGCGCAGGAG *F *F Response: *F *F Concerning D459: *F *F The C is confirmed by 9 reads, 3 forward and 6 reverse. They are derived *F from 4 different subclones. The BAC was completed independently of any *F other clones. *F # *U FBrf0111659 *a Bernstein *b S. *c S. *d Zhang *t 1999.11.18 *T personal communication to FlyBase *u *F Personal Communication from: Sandy Bernstein, Shuxing Zhang *F San Diego State University *F San Diego, CA *F Date: Nov. 18, 1999 *F *F *F Query from curator: *F *F I am working on the annotation of the Mhc genomic sequences from BDGP. *F I would like to be able to put as many complete mRNAs and CDSs on the *F sequence as possible but have only found the structures of six *F completely characterized mRNAs in the literature. *F *F I have found reference to the mRNAs containing the following variable exons *F (In the nomenclature of George et al. which I came accross first) in the *F literature *F *F From George et al. 1989 *F 1) 3b, 7a, 9b, 11d, 15b *F *F 2) 3b, 7d, 9b, 11b, 15a *F *F *F From Wells et al. 1996 *F 3) 3a, 7a, 9b, 11c, 15b *F *F 4) 3b, 7a, 9b, 11c, 15b *F *F 5) 3a, 7a, 9c, 11d, 15b *F *F 6) 3b, 7a, 9b, 11a, 15b *F *F All of the above use exons 17 and 19 and do not contain exon 18. *F *F In the Bernstein and Milligan JMB 1997 paper, it says that 12 *F combinations of alternative exons are known. If you could provide me *F with the structure of the additional complete forms, I will include *F them in the annotation. *F *F Also, a point of clarification: *F Am I interpreting the statement in Wells et al. "Note that the exon 11 *F series is designated in linear order as a-e, rather than by the order *F used in George et al. (1989)" correctly to mean that the *F correspondence between the exons in the Wells et al. and the George et *F al. papers is as follows? *F *F George et al. Wells et al. *F *F 11e 11a *F 11a 11b *F 11b 11c *F 11c 11d *F 11d 11e *F *F *F One last question. *F I am also including the positions of as many mutations, insertions, *F etc. as I can in the sequence. In Kronert et al. 1999. J.Cell Biol. *F 144(5):989-1000, the positions of the mutations in Table 1 are given in *F terms of the numbering of the chicken amino acid sequence. As a result, *F I am having a hard time locating them in the Drosophila sequence. In *F all cases, I have found the appropriate residue or residues within 4 *F amino acids of the stated position in the chicken sequence but don't *F know if these are the correct positions. In one case (Mhc[D45] there *F are two possible Ala codons (GCT) within three amino acid positions aa *F 261 (the stated position). I have tentatively mapped the mutations to *F the following Drosophila codons: *F *F Mhc mutation chick codon Drosophila codon (?) *F *F D1 625 623 *F D41 328 324 *F D45 261 258 0r 260 *F D62 638 (start) 637 (start) *F 5 200 200 *F 8 832 829 *F *F Can you either confirm these positions or point me to a good source for *F the chicken numbering you are using? *F *F *F Reply: *F *F 1) The isoform compilation is in Table 2 from a manuscript we are *F preparing. Note that some are derived from cDNAs, others are from in situ *F hybridization. You probably don't want to list all the details of the *F embryonic forms. Probably somatic, cardiac, visceral or pharyngeal muscle *F would be the best description. Listing the new ones as "personal *F communication" would be OK (the co-author on the manuscript is Shuxing *F Zhang). *F *F 2) Your exon 11 designations are correct. Note, however, that Charlie *F Emerson is adamant that we use the George et al designations since they *F were published first. We are now doing so. Thus the table I attached uses *F the George designations. I suggest you use George et al's nomenclature, *F since this is what I have agreed to do. *F *F 3) The numbers you cite for the Drosophila mutations are correct. D45 *F should be Drosophila codon 258. *F *F *F Text version of Table 2 *F Stage and Tissue-specific use of Mhc Alternatative Exons in *F Drosophila *F *F Alternative Exons used in Embyonic Muscles/ cDNAs *F *F Mhc form *F Exons 3a, 7a, 9b, 11c, 15b, 18 excluded *F Tissues/cDNA *F Dorsal Acute 3 *F Longitudinal Oblique *F Lateral Longitudinal 1 *F Lateral Oblique *F Ventral Longitudinal 1-4 *F Segment Border *F Dorsal Oblique 3, 4, 5 *F Ventral Oblique 1, 2, 4-6 *F Embryonic cDNA c21 *F *F Mhc form *F Exons 3b, 7a, 9b, 11c, 15b, 18 excluded *F Tissues/cDNA *F Ventral Acute 3 *F Dorsal Acute 3 *F Dorsal Oblique 1, 2, 3 *F Segment Border *F Embryonic cDNA c61 *F *F Mhc form *F Exons 3a, 7a, 9b, 11d, 15b, 18 excluded *F Tissues/cDNA *F Dorsal Acute 1, 2 *F Ventral Oblique 1-6 *F Heart Cardiobast *F *F Mhc form *F Exons 3b, 7a, 9c, 11d, 15b, 18 excluded *F Tissues/cDNA *F Transverse (all 6) *F Dorsal Acute 1, 2 *F *F Mhc form *F Exons 3a, 7a, 9c, 11d, 15b, 18 excluded *F Tissues/cDNA *F Dorsal Acute 1, 2 *F Embryonic cDNA c52 *F *F Mhc form *F Exons 3b, 7a, 9c, 11c, 15b, 18 excluded *F Tissues/cDNA *F Ventral Acute 1, 2 *F *F Mhc form *F Exons 3a, 7b, 9b, 11d, 15b. 18 excluded *F Tissues/cDNA *F Pharyngeal *F *F Mhc form *F Exons 3b, 7a, 9b, 11a, 15b, 18 excluded *F Tissues/cDNA *F Embryonic cDNA c51 *F *F *F Alternative Exons used in Pupal and Adult Muscles/ cDNAs *F *F Mhc form *F Exons 3b, 7d, 9a, 11e, 15a, 18 included *F Tissues/cDNA *F Indirect flight muscle *F *F Mhc form *F Exons 3b, 7d, 9a, 11b, 15a, 18 included *F Tissues/cDNA *F Jump muscle-small cells *F *F Mhc form *F Exons 3b, 7d, 9a,b 11b, 15a, 18 included *F Tissues/cDNA *F Jump muscle-large cells *F *F Mhc form *F Exons 3b, 7d, 9b 11b, 15a, 18 included *F Tissues/cDNA *F Direct flight muscle #51 *F *F Mhc form *F Exons 3b, 7a, 9b 11c, 15b, 18 excluded *F Tissues/cDNA *F Direct flight muscle #52 *F Esophagus (adult) *F *F Mhc form *F Exons 3 N.A., 7 N.A., 9b, 11 N.A., 15a,b, 18 excl./incl. *F Tissues/cDNA *F Leg *F *F Mhc form *F Exons 3 N.A., 7 N.A., 9b, 11 N.A., 15a,b, 18 excluded *F Tissues/cDNA *F Proboscis *F *F Mhc form *F Exons 3 N.A., 7 N.A., 9b or c, 11 N.A., 15b, 18 excluded *F Tissues/cDNA *F Body wall *F *F Mhc form *F Exons 3b, 7d, 9b 11b, 15a, 18 excluded *F Tissues/cDNA *F Pupal cDNA cD301 *F *F Mhc form *F Exons 3-, 7a, 9b 11d, 15b, 18 excluded *F Tissues/cDNA *F Pupal cDNA cD302 *F *F Table 2 legend: N.A., not analyzed. Embryonic tissue localization from *F the present study. Embryonic cDNAs from Edwards (1990) and Wells et *F al., 1996. Pupal/adult tissue localization from Kazzaz and Rozek *F (1989), Collier, et al. (1990), Edwards (1990), Hastings and Emerson *F (1991), Kronert et al. (1991). Pupal cDNA clones are from George et *F al., 1989. *F *F # *U FBrf0111660 *a Kramer *b H. *t 1999.11.14 *T personal communication to FlyBase *u *F *F Date: 14 Nov 1999 *F From: Helmut Kramer *F *F "I agree with the following corrections concerning the 4 hook alleles in *F Table 1 of Genetics 151:675-684 (FBrf0106787): *F *F hk[7] altered sequence position is at bp 1014, not 2014 *F hk[8] altered sequence position is at bp 1191, not 1991 *F hk[9] altered sequence position is at bp 1402, not 1403 *F hk[16] altered sequence position is at bp 1472, not 1572" *F # *U FBrf0111661 *a Kaufman *b T. *t 1999.12.1 *T personal communication to FlyBase *u *F *F Personal Communication from: *F *F Thom Kaufman *F Indiana University *F Bloomington, IN *F Dec. 1, 1999 *F *F Regarding definition at the molecular level of the Cnn rescue construct *F described in Li et al. J. Cell Biology paper, the distal end of the phage *F insert is located at approximately base 82238 of the BDGP 2R 50A1-50B1 *F sequence file and the proximal end is defined as the Sal I restriction site *F at base 100,005 of the same file. The BDGP 2R 50A1-50B1 sequence file has *F accession numbers AC007452, AC005052, AC006488, AC005986 and NID number *F g4731053. *F # *U FBrf0111662 *a Kim-Ha *b J. *t 1999.12.2 *T personal communication to FlyBase *u *F *F Personal communication from: *F *F Jeongsil Kim-Ha *F Sungkyunkwan University School of Medicine *F Center for Transcriptional Mediator *F Seoul, Korea *F Dec. 2, 1999 *F *F Query from Curator: *F *F I have a couple of questions with respect to the data in Kim-Ha et al., 1999, *F Molec. Cell. Biol. 19(4): 2505--2515. *F *F 1) Can you provide me the boundaries (restriction sites?) of the 17.1kb *F genomic fragment used for rescue so I can find it in the sequence? *F *F 2) Can you give me the exact location of the P{lacW}Rbp9[k12901] *F insertion so I can include it in the annotation. *F *F 3) When you say that other P elements have landed at a particular base *F (e.g. P{lacW}Rbp9[P1374] inserted at nucleotide 4668), do you mean that *F it inserted between 4667 and 4668 or between 4668 and 4669? *F *F *F Response: *F *F The informations you requested are as follows: 1) The 5' restriction *F enzyme site is SalI and we didn't sequence this region. The seq. of 3' *F site is 5'-ATGTGTGTAGTGCATAGAGAAACCG-3'/end 2) We do not know the exact *F location of the P{lacW} Rbp9[k12901] as we did not sequence the *F junction. We know that it is located roughly at nucleotide position *F 3400 by analyzing the PCR product. 3) P[2775], P[2690] and P[1374] are *F inserted between 7132-7133, 7197-7198 and 4668-4669, respectively. *F # *U FBrf0112118 *a Cornell lab *b ?. *t 1999.7.25 *T personal communication to FlyBase *u *F http://www.mbg.cornell.edu/ July 25 1999 *F *F *F *F Drosophila melanogaster *F *F MICROSATELLITES *F *F (Schug, et al. (ref. 5)) *F *F Note: Heterozygosities are based on various sample sizes and from population *F samples that differ dramatically in geographic distribution. They are not *F meant to be used as population estimates, but rather as a rough guide to *F gauge the potential usefulness of each marker. *F *F This page maintained by Malcolm Schug (mds25@cornell.edu) and Chip Aquadro *F (cfa1@cornell.edu) *F *F GenBank *F Locus Repeat Cyt. Genetic accession PCR Het. No. Anneal MgCl2 *F locationlocation *F no. Positionsize allelestemp. [mM] Primer (For./Rev.) Ref. *F X *F Chromosome *F DROACS2 (CT)7 1B1-5 0 M17119 444 141 0.51 3 57 1.5 cactcacttcgagttccctacc 4 *F ctaaaccctcgcgttatgtacc *F DROZFP (TATG)4 2D2-3 0 M64750 16629 148 0.04 2 53 2.5 tctcggcaataaaatccctagc 4 *F ttagttggttatttccctctttcg *F DROPCXGEN (AAAC)5 2E2 0.9 M74329 16080 145 0.70 5 57 1.5 actgtacgccttttccatgg 5 *F tgcatacactaaacgcagtcg *F DMZW3K25 (AT)14 3A 1 X54006 3068 101 0.85 8 55 1.4 attgtcattttattgctgccg 1 *F taacgaagagagttgcggaga *F DMSGG3 (CAG)11 3B1 1.3 X70862 2304 124 0.80 4 55 1.4 tccagcaatcacagcaaact 1 *F tcttttgaaattgcggttga *F DS06335a (AC)7 3C1-3C6 81-141 0.75 12 53 actgtaattgctgttctatgt 6 *F ctcacactgggacacaaaa *F DS06335b (GT) 3C1-3C5 134-147 0.31 6 50 gcacaatcacatcgtattcact 6 *F attgttgttgctgcgattt *F DMWHITE (TA)13 3C2 1.5 X02974 5729 238 0.88 13 55 2.5 cacatacacagatttattgagccc 4 *F acacacacttttatactctctccgc *F DS06577 (GT) 3C7 122-145 0.61 8 54 tttgcggcttcgtttgttta 6 *F gatccgcccacatacacact *F DELTEX (AGTT)6 6A1-2 17 U09789 2861 172 0.58 3 57 1.5 tacgcaataagttggcgta 5 *F aatcaggataatgcctaatactagt *F DROOTUA (TA)9 7F1 23.2 M30825 5039 134 0.76 4 55 1.4 tcacgaaatcaaagaaccacc 1 *F gtctctagccgaacagcgag *F DROSOR1 (TAT)6 8D 27.6 D13782 1600 111 0.16 2 57 1.5 tttgttcaatagagcgttgacg 4 *F agaacaccccatcatacatgg *F DROSEV2 (TTG)9 10A1-2 33.38 J03158 1890 100 0.72 7 53 1.5 catcttaatgaggataaatttgttat 1,5 *F aagcgacaagtttcaattaac *F DROSEV1 (GT)13 10A1-2 33.38 J03158 1183 145 0.50 2 57 1.5 atacaagatacatccgtgcgc 4 *F cccaactgaaaagcaactcc *F DMTENA (AT)14 11A6-9 38 X68794 3040 98 0.86 5 55 1.4 ctgttagtgcgcagggattc 1 *F acgtgtgcgtgttgtttctc *F DROYTD3 (ACG)5 12B-C 44 M15898 1720 201 0.72 10 53 1.5 atccggctatttgcaatc 5 *F tctggcttatatagctcc *F DRORUD4 (AATT)5 15A1-2 54.5 M24584 196 148 0.65 3 53 1.5 tggaaccatgttctatatgcc 5 *F tgaagatgtccagcagcg *F DROEXO2 (CATA)8 13F 58 L07660 1963 135 0.39 3 57 1.5 tgcagggcaccttctctc 4 *F gaacgcttgatttagatttggg *F DMTROPONI (CA)11 16F3-6 X58188 1231 99 0.75 5 55 1.4 caagagatcccgagagagaga 1 *F acgtgtgcgtgttgtttctc *F DMAC2 (AC)12 104 0.18 3 60 2.5 cccattgattttgttgtgtaag 2 *F gcacatgcatgtgggtgt *F DMAC4 (AC)9+10 170 0.67 6 60 2.5 agaattcgaagtgcaagacca 2 *F tgtgggcataaccaacacat *F *F 1. Goldstein D.B. and A.G. Clark (1995) Microsatellite variation in North *F American populations of Drosophila melanogaster. Nucleic Acids Res.23, *F 3882-3886. *F *F 2. England,R., D.A. Briscoe, and R. Frankham (1996) Microsatellite *F polymorphisms in a wild population of Drosophila melanogaster. Genet. Res., *F Camb.67, 285-290. *F *F 3. Michalakis, Y., and M. Veuille (1996) Length variation of CAG/CAA *F trinucleotide repeats in natural populations of Drosophila melanogaster and *F its relation to the recombination rate. Genetics 143, 1713-1725. *F *F 4. Schug, M.D., T.F.C. Mackay, and C.F. Aquadro (1997) Low mutation rates of *F microsatellite loci in Drosophila melanogaster. Nature Genetics,15:99-102. *F *F 5.Schug, M.D., K.A. Wetterstrand, M.S. Gaudette, R.H. Lim, C.M. Hutter, C.F. *F Aquadro (1998) The distribution and frequency of microsatellite loci in *F Drosophila melanogaster. Molecular Ecology 7:57-70. *F *F 6.Schlotterer, C., C. Vogl, D. Tautz (1997) Polymorphism and locus-specific *F effects on polymorphism at microsatellite loci in natural populations of *F Drosophila melanogaster populations. Genetics 146: 309-320. *F Chromosome *F II *F *F DROEXPAND (CAG)8 21C3 0.1 L14768 5140 90 0.81 5 55 1.4 gtgatcgatcccgctgtc 1 *F tccggtttccaattagcttg *F DROYANETSB (TG)19 22C 4 M97694 3624 106 0.91 9 55 1.4 taatggggaatgggtgaatg 1 *F gccgtgctcttttctcttacg *F ODD (CAG)4 24A 8 X57480 457 187 0.08 4 60 2.5 atgcgacacatggcccacta 3 *F ttgcggatccagtggttcat *F DROGPDHA (CT)7 25F5-26A17.8 J04567 1220 134 0.33 1.7 53 1.5 cattggaaaagtgagcggat 5 *F cggacaacaacaaatcgttg *F DRONINAC (AT)10 28A1-3 22 J03131 5673 139 0.41 1.9 56 1.5 tttgtcaatctctcacagcagg 5 *F gcccgagtacatttattcaagc *F BIB (CAG)5 30F 34.7 X53275 2293 151 0.72 6 60 2.5 tcgcaaggatcagcggtgac 3 *F ttgggcctcagcggcagaat *F DS06238 (GT) 35B1-B3 131-138 0.53 8 52 gggcattaatctgcattttcta 6 *F ttcattagtaattgcaggcact *F SU(H) (CAG)6 35B 50.8 M94383 635 259 0.63 6 60 2.5 aacggctcacccctcgatcc 3 *F tacttctccatggcgtcccg *F DROMHC (AC)13 36A7-C1 108 M61229 7136 109 0.8 4 57 1.5 aaacccacacaacaactgca *F gacattaccgatattggatgca *F DL (CAG)9 36C 52.9 M23702 1655 200 0.72 12 60 2.5 ctgatgtcgcccaaccatcc 3 *F ccgccaggattgccaaaggg *F DS08513 (GT) 37B8-C2 192-205 0.51 5 54 ctacaccatgccctgaaaag 6 *F gacggcgtgaccatgtctg *F CAD (CAA)10 38E 54 M21069 1655 200 0.77 9 60 2.5 tcgggctcggaaatctctag 3 *F aagtatggcggcttcgatgg *F DROTG121 (ATTC)6 42A 56 J01139 404 196 -- -- 57 1.5 tccaagcagtgcatcaactagc 4 *F cagtcctcgcttttgtatgagg *F ADF1 (CAG)4 42C 56 M37787 670 130 0 1 60 2.5 tatcgagagtcgctgctcgg 3 *F ctgccgttgaagggctgtgc *F DROGPAD (GT)19 47A 60 M31129 764 185 0.68 11 56 2.5 gaaataggaatcattttgaatggc 4 *F aattaaaaacaaaaaacctgagcg *F SU(Z)2 (CAG)7 49C 67 X56798 5085 181 0.53 8 60 2.5 atcaatagcctggtggtggg 3 *F gatgtgggcaaggttaggtc *F DMMP20 (CA)10 49F9-13 68 Y00795 656 93 0.77 4 55 1.4 catgcaaatgagcagtactttg 1 *F tattttcacacatttccaatcg *F DMS2ZSTM (AAT)5 49E-F 67 X56799 4654 133 0.08 1.2 56 2 ccaagcaaatttcaaaaatgc 5 *F gctttcgaaaatgccagc *F DMMASTER (CAG)8 50C 70.3 X54251 958 86 0.76 5 55 1.4 cagcagcagatccaagttca 1 *F gtttgcattgtagggcgagt *F MAM (CAG)8 50C 70.3 X54251 958 277 0.53 7 55 1.4 ggcggcctaccagttttcca 3 *F cctgttgctcccaggtttgc *F DS00062 (GT) 54A1-B2 158-174 0.71 12 53 acgggaacgccatctaac 6 *F agaagagaccctgcaacaca *F DS00361 (GT) 54B1-B2 132-159 0.59 10 52 caaccacccacaagcacac 6 *F caaccacccacaagcacac *F DMELF (CAG)7 54F 87 X15657 404 84 0.97 4 55 1.4 acagcaacaacggagcaac 1 *F tctgcaacctgggagtctg *F ELF1 (CAG)6 54F 87 X15657 2343 112 0.47 5 60 2.5 tccacgacaacgatctcgca 3 *F ctaacaatgtcgccgggatg *F DS08687a (GT) 57C5-D1 178-187 0.55 8 54 tgatttggactgagatcagg 6 *F gcccaacgaatcatttcac *F DS08687b (GT) 57C5-D1 155-174 0.72 13 52 tgtcgagagcagcagcagt 6 *F ttgccttccctgttacag *F TWI (CAG)3 59C-D 102 X12506 353 226 0.06 3 60 2.5 aaagtgctgctggacataag 3 *F ataccataggcggcgtactc *F SLOBO (CAG)4 60C-D [108] L00632 3100 311 0.65 5 60 2.5 atgcttaacatggagtcgcc 3 *F tgcagcatctgcttctccag *F DMAC1 (AC)12 II -- -- -- 139 0.65 5 60 2.5 tacacctgcgtgcccaac 2 *F ggggctaatggagcctagtg *F DMAC8 (AC)9 II -- -- -- 95 0.51 6 60 2.5 cccgcatcaccacaacac 2 *F caagtgtcacaatgtcgatgg *F DMAC9 (AC)13 II -- -- -- 167 0.71 10 60 2.5 ctatacgcgcatcgacgtc 2 *F tggctacactatccagcgc *F *F 1. Goldstein, D and A.G.Clark(1995) Microsatellite variation in North *F American populations of Drosophila melanogaster. Nucleic Acids Res.23, *F 3882-3886. *F *F 2. England,R., D.A. Briscoe, and R. Frankham (1996) Microsatellite *F polymorphisms in a wild population of Drosophila melanogaster. Genet. Res., *F Camb.67, 285-290. *F *F 3. Michalakis, Y., and M. Veuille (1996) Length variation of CAG/CAA *F trinucleotide repeats in natural populations of Drosophila melanogaster and *F its relation to the recombination rate. Genetics 143, 1713-1725. *F *F 4. Schug, M.D., T.F.C. Mackay, and C.F. Aquadro (1997) Low mutation rates of *F microsatellite loci in Drosophila melanogaster. Nature Genetics, 15:99-102. *F *F 5.Schug, M.D., K.A. Wetterstrand, M.S. Gaudette, R.H. Lim, C.M. Hutter, C.F. *F Aquadro (1998) The distribution and frequency of microsatellite loci in *F Drosophila melanogaster. Molecular Ecology 7:57-70. *F *F 6.Schlotterer, C., C. Vogl, D. Tautz (1997) Polymorphism and locus-specific *F effects on polymorphism at microsatellite loci in natural populations of *F Drosophila melanogaster populations. Genetics 146: 309-320. *F Chromosome *F III *F *F DMRHOb (AC)6 62A 32 X52454 2273 113 0.83 7 56 2.0 tatactaaagtcacttaatgcgttaca 4 *F gtttgtgtgaatttcactaaattatta *F DMCPDR (ACC)5 62B4-5 1.5 I32839 939 372 0.56 3 55 2.5 tatcctatgcaaacacaggcc 5 *F ggccataactgaaaagctatgc *F DMHSP82 (TA)5 63B9 5 X03810 4632 229 0.59 3 55 2.5 actaaagactacatacgcgccc 5 *F gagtgtccacggtggagg *F DMDSRC (TA)7 64B 15 K01043 1051 319 0.53 2 55 2.5 gacaacatttatcagctgctgc 5 *F agagttcatgtgtgttggttgg *F DMPOUC (AGC)6 65D1-3 22 M81959 2129 143 0 1 55 2.5 actatcagcaccaccatcacc 5 *F gccaaagctgttaaagtgcc *F DROHSP1 (CAGC)5 67B [35] M26267 473 149 0.23 5 57 2.5 tttgtagcttccaggcgg 4 *F ctgaatttccactgccgg *F DROLAMB2A (ATT)5 67C 28 M58417 4925 228 0.22 2 55 2.5 cgtaggaaggaaagaaatcgg 5 *F aatttgcagttgataggcagc *F DROECT (ATTT)9 67D8-10 38 M14740 746 315 0.49 2 57 1.5 gaagaaatagagaattgttaaacctt 4 *F ctaatgatgagtgtgagt *F DMSGS378 (AC)8 70C2 41.4 X10918 6205 128 0.42 5 53 1.5 gaaagagctccaaggcaatcagg 5 *F tgtttcccaggacaggataagcg 5 *F DMZ60MEX (TTC)8 71C-D 43 X58286 3348 95 0.79 6 55 1.4 aaatctgttgctcatactgccc 1 *F aaccggcgaaatgttcag *F DROTKABL3 (ACA)5 73B 41 M19692 3610 134 0.08 2 57 1.5 tcaagacccaaattccaacc 4 *F tttctgctgttgttgatgtgc *F DMCATHPO (ACC)6 75D-E1 46 X52286 1685 130 0.1 3 53 1.5 ttcgacggatcagacttggtttttggc 4 *F gcgttcgcctttcttagtcaatttcgg *F DRO15DC96Z(AAAT)5 84A1-2 47 L32725 60 206 0 1 57 1.5 attataatttcatgaccagtttc 4 *F taattaccttccacttaaatgtttcta *F DMANTPE1 (CCG)5 84B1-2 47 X03787 1242 171 0 1 57 1.5 ataagactttatttatatactactcgc 4 *F gtgtgatgttgatttctt *F DRO17CD2Z (CT)9 84 47 L32209 3409 131 0.61 9 53 1.5 ttcgtgcaaaggtgttttcc 5 *F atgcagataccagaaaccgc *F DRO17DC2Z (AC)14 84 47 L32209 1810 137 0.52 7 55 5.0 cacacaacgactggctgg 5 *F tctgtgtcacgtgcacaaatgg *F DRO17DC4Z (AC)9 84 47 L32208 2016 144 0.56 6 55 5.0 ttcgtttttgaattattgcgc 5 *F cctgttcgtccaactatgtgc *F DROSEQA (GA)4 84 47 M95823 2677 148 0 1 53 1.5 ttgtgttgttgttctttttccg 5 *F gccaacaacaaatcacatgc *F DRO13DC98Z (CA)8 84 47 L32633 2411 125 0 1 53 1.5 aaaaggatcctggcgacc 5 *F atgtccatgtccggttgc *F DRO17DC2Z (AAT)5 84 47 L32209 2393 145 0 1 53 1.5 atgctacactcaaacaaatggg 5 *F ttggaaccaaggatcttactgc *F DRO14DC95Z (GT)7 84 47 L32654 1507 117 0 1 55 5.0 agaacgtttagaatcaaccg 5 *F ttcgaactaccgctctttcg *F DRO11DC7Z (GAT)7 84 47 L32735 2238 149 0.27 3 53 1.5 ggaaagtctcaaggaaacgc 5 *F agacgctattatttccgacagc *F DRO15DC97Z (AG)12 84 47.4 L32726 2559 95 0.59 4 55 1.4 tgcattgctaatgatcgtgg 1 *F gctttttcttacacaattcgca *F DMPROSPER (GA)12 86E1 50 Z11743 5337 205 0.70 7 57 2.5 cggtacaaagtgtgtgttc 4 *F gacttttaaacatttaagattaattcc *F DROSVP (AGC)5 87B4-5 51 M28863 2326 145 0 1 55 2.5 agtcatgaccagctcgcc 5 *F tgccgtagttgttgatctgc *F DMTMII (CA)12 88F2-3 55 X76208 9365 244 -- -- 53 5.0 taattatcgctatgtagac 5 *F cttctacatttaatttgaa *F DROTROPI1 (TA)14 884F-5 55 K03276 437 268 0.86 17 57 2.0 aaatggatttagcgaggtcg 4 *F gttccccgtttgattttttg *F DROFASI (AGG)5 89E-F 58 M32311 1707 139 0.35 4 57 1.5 gatgattcggaactctctcagg 4 *F tttgcaccaactgtttctgc *F DROABDB (CA)19 89E1-2 59 L07835 49028 148 0.76 10 57 1.5 cttccaagtcacacggac 4 *F cacaccgacaacacaagacc *F DMEHAB (AGCC)5 90B1-2 59 X72303 218 361 0.27 5 56 2.5 atttattagttttttgctaatacttgc 4 *F agagttcttgttgtatttatac *F DMCPO17G (AGC)5 90D1-2 62 Z14311 1252 275 0.60 3 55 2.5 tcgcacgagtccaactcc 5 *F acggagtccatgctctgc *F DRONANOS (TA)18 90E-F 67 M72421 3143 130 0.88 23 55 2.0 cgcaagtattcatttcaacaca 1 *F tgctggcggttgtttcat *F DMGLASS (TG)8 91A1-2 63.1 X15400 5327 285 0.64 4 55 2.5 gcaccattgcacagatatgc 5 *F tcttccagggaatctgct *F DROHOXNK4 (AGC)5 91E1-3 72 M27292 510 143 0.13 2 55 2.5 ctgaagttgaagtccgagcc 5 *F tacatgtgctgcatctgttgc *F DMU1951 (TA)16 93C 82 X53543 1948 198 0.94 8 55 1.4 gggtctttctgcttcagttacc 1 *F ggaatacacgaatccccctt *F DMPOINT1Z9 (ATC)7 94F1-2 79 X69166 1270 129 0.56 2 55 2.5 caataacaattgccacacgg 5 *F aattggtgatgcggatgg *F DMSIDNA (GC)6 96E1-4 87 X79340 3334 126 0.42 3 55 2.5 tgcacatactcctttcaattcg 5 *F aacaggactcggaacaatgc *F DMALD (AGT)5 97B 91.5 X60064 2854 136 0.43 2 55 2.5 attattaccacccatgctttcg 5 *F cataacgagattttaggtccgg *F DROLMALK (CAA)6 98 98 L37323 855 165 0.91 6 55 1.4 gggtaatcccttgctaatatgg 1 *F aatggttgtcgctaaaagtt *F DROF1GA (AAC)5 100E 102 M11744 1610 308 0.25 2 55 2.5 gctgtgcgtgacatgagg 5 *F tgctgaaacatcatcttatcgg *F DMAC3 (AC)9 III -- -- -- 196 0.33 3 60 2.5 ctccacaatccaccctcg 2 *F ccatctaccacacaaccgc *F *F 1. Goldstein, D. and A.G.Clark(1995) Microsatellite variation in North *F American populations of Drosophila melanogaster. Nucleic Acids Res.23, *F 3882-3886. *F *F 2. England,R., D.A. Briscoe, and R. Frankham (1996) Microsatellite *F polymorphisms in a wild population of Drosophila melanogaster. Genet. Res., *F Camb.67, 285-290. *F *F 3. Michalakis, Y., and M. Veuille (1996) Length variation of CAG/CAA *F trinucleotide repeats in natural populations of Drosophila melanogaster and *F its relation to the recombination rate. Genetics 143, 1713-1725. *F *F 4. Schug, M.D., T.F.C. Mackay, and C.F. Aquadro (1997) Low mutation rates of *F microsatellite loci in Drosophila melanogaster. Nature Genetics, 15:99-102. *F *F 5.Schug, M.D., K.A. Wetterstrand, M.S. Gaudette, R.H. Lim, C.M. Hutter, C.F. *F Aquadro (1998) The distribution and frequency of microsatellite loci in *F Drosophila melanogaster. Molecular Ecology 7:57-70. *F *F 6.Schlotterer, C., C. Vogl, D. Tautz (1997) Polymorphism and locus-specific *F effects on polymorphism at microsatellite loci in natural populations of *F Drosophila melanogaster populations. Genetics 146: 309-320. *F *F Unlocalized *F DMAC6 (AC)13 -- -- -- -- 315 0 1 60 2.5 ctgttcttctgccgttgtca 2 *F gagctcggtacccctactcc *F DMAC7 (AC)10 -- -- -- -- 219 0.60 5 60 2.5 gtgattctcaacaaagcgca 2 *F gcagatgtttcaccccttgt *F DMAC10 (AC)13 -- -- -- -- 273 0 1 60 2.5 tgactacggtgccatttcaa 2 *F gagcccaacgcaattaatgt *F DMAC12 (AC)11 -- -- -- -- 111 0.25 5 60 2.5 gtgacttccggcaatgtttt 2 *F cggtacccctgctattacca *F DRO36DC51Z (AC)8 -- -- L77000 3169 151 -- -- 53 1.5 gatccccattggtagtcgtatttc 5 *F gcccgcagccaagatgttaaaaac *F DM28 (AC)12 -- -- -- -- 123 0.49 6 62 6.7 tttactcgtcgcgcagtctgtcg *F tatctatatgcacatgtcacc *F DM38 (AC)10 -- -- -- -- 111 0.59 6 53 2.5 accttttcattttccacccc *F ggcctgaaagtaggcaacac *F DM40 (AG)13 -- -- -- -- 239 0.85 9 62 1.5 acaatggttggatgtggatgtgg *F *F DM42 (AC)9 -- -- -- -- 401 0.48 5 53 1.5 tgctccgtctgtccgtgatacccg 5 *F ccattagactgattttgttgcg *F DM54 (AC)12 *F *F DM55 (AC)10 -- -- -- -- 161 0.47 5 53 1.5 agcgcgactcacagatactctgg 5 *F cgagactgaaatcgacgctgaag *F DM58 (AC)11 -- -- -- -- 276 0.69 8 57 2.5 tgaaagatttcgacgctcaa *F gctctatgcccgtctctgtc *F DM73 (AC)21 -- -- -- -- 272 0.95 15 53 1.5 ggaagaacaaggcttcagatacg 5 *F DM75 (AC)12 -- -- -- -- 127 0.68 7 62 2.5 tgtcccgtttacagcaacaa *F caactgaaatgcgaaacgtc *F tttcgcttcattcatgggcacgg *F DM78 (AC)10 -- -- -- -- 179 0.44 5 53 1.5 gaaagagctccaaggcaatcagg 5 *F tgtttcccaggacaggataagcg *F DM80 (AC)9 -- -- -- -- 257 0.56 6 57 1.5 tgattcccatgctctgccgactg 5 *F caaccgaactgaactctactgaacaaa *F DM86 (AC)9 -- -- -- -- 189 0.67 10 60 1.5 tccatcattgactggtcagc *F ggcctgaaagtaggcaacac *F DM87 (AC)10 -- -- --- -- 138 0.19 3 61 2.5 tggccctggacgtgctttgtatcc *F aaaggtaacctggtccaccc *F DM88 (AC)7 -- -- -- -- 109 0.77 8 60 1.5 agaaaagcgctcaagctgtc *F tttagaggcagccgaaaatgc *F DM89 (AC)14 -- -- -- -- 128 0.42 5 53 1.5 ctttaccgagtatctgtgcgc *F gagccgattcttgagattgc *F DM94 (AC)9 -- -- -- -- 168 0.85 12 60 1.5 gcccagtattcgtatctgaagg *F gatgagaaagtggaaggactcc *F DM95 (AC)10 -- -- -- -- 185 0.62 7 53 2.5 caggcaatcaaactccatca *F cacaacatttttattcgccc *F DM97 (AC)30 -- -- -- -- 157 0.82 12 58 1.5 aaataagcctcgcttcggtt *F ggagagctcttcacgctgat *F DM98 (AC)10 -- -- -- -- 189 0.62 6 58 2.5 gtcgactttacggcggtccagtgc *F aggaagaacaacaacggtcg *F DM100 (AG)14 -- -- -- -- 167 0.74 7 62 1.5 gaaaagcgctcaagctgtct *F gtctcggtatataaggagtcagc *F DM114 (AC)8 -- -- -- -- 260 0.78 6 53 1.5 ccaaagccagggacagactcaggc 5 *F aaccgttgggcaaatgttcaacg *F DM116 (AC)7 -- -- -- -- 233 0.13 4 53 2.5 atgagccgagttgctgttcgcagg 5 *F actcacatcctccattccgatgg *F DM122 (AC)12 -- -- -- -- 199 0.48 6 53 2.5 tcccgatttctcgcagttac *F ttccgagggagaagcaagta *F DM123 (AC)6 -- -- -- -- 142 0.61 6 60 1.5 gcattgtcagcggataacaga *F cagtgtgggttagctcagc *F *F *F 1. Goldstein and A.G.Clark(1995) Microsatellite variation in North *F Americanpopulations of Drosophila melanogaster. Nucleic Acids Res.23, *F 3882-3886. *F *F 2. England,R., D.A. Briscoe, and R. Frankham (1996) Microsatellite *F polymorphismsin a wild population of Drosophila melanogaster. Genet. Res., *F Camb.67, 285-290. *F *F 3. Michalakis, Y., and M. Veuille (1996) Length variation of *F CAG/CAAtrinucleotide repeats in natural populations of Drosophila *F melanogasterand its relation to the recombination rate. Genetics 143, *F 1713-1725. *F *F 4. Schug, M.D., T.F.C. Mackay, and C.F. Aquadro (1997) Low mutationrates of *F microsatellite loci in Drosophila melanogaster. NatureGenetics, 15:99-102. *F *F 5.Schug, M.D., K.A. Wetterstrand, M.S. Gaudette, R.H. Lim, C.M. Hutter, C.F. *F Aquadro (in review) The distribution and frequency of microsatellites in the *F genome of Drosophila melanogaster. *F *F *F http://www.mbg.cornell.edu/ July 25 1999 *F *F Drosophila pseudoobscura microsatellites *F *F All amplifications done with MgCl2 conc. 1.5mM *F *F Microsatellites were isolated by screening a genomic DNA library (DPS****) *F or through GenBank screens. *F *F _____________________________________________________________ *F *F Name... Chr.... Size..... Annealing Temp.... Primers *F *F _____________________________________________________________ *F *F *F *F DPSX001..X.......200..........61.0.......gaatctctctcctgttgcgg *F .........................................ccacactcgctttcccata *F *F DPSX003..X.......200..........61.0.......gcctacagtgagagctgcct *F .........................................tggggagtggacttatctcg *F *F DPSX004..X.......100..........58.0.......aagtacttcattttgtcttgg *F .........................................cgtgcgcgcttataattctt *F *F DPS2001..2.......200..........61.0.......caaagacagagccaaagcct *F .........................................tgggcattaaagtgcaatca *F *F DPS3002..3.......300..........61.0.......gagtccccaaaatccgaaac *F .........................................cccacaacggacagaaaaat *F *F DPS3003..3.......250..........58.0.......ggcccgaaaataaaacaaca *F .........................................ctgcactctctttccccctt *F *F DPS4001..4.......300..........61.0.......gtctgctgcgattaaaagcc *F .........................................cggcaggcggtataaaaata *F *F DPS4002..4.......100..........61.0.......taccgtatgcaacccagctt *F .........................................cggaatgcactctgctgata *F *F _____________________________________________________________ *F *F *F *F Back to Mohamed Noor homepage. # *U FBrf0112119 *a Cornell lab *b ?. *t 1999.7.27 *T personal communication to FlyBase *u *F http://www.mbg.cornell.edu/ July 25 1999 *F *F Drosophila simulans *F *F dinucleotide repeat microsatellites *F *F and assay conditions for PCR *F *F *F *F from Hutter, Schug, and Aquadro 1998. Microsatellite variation in *F Drosophila melanogaster and D. simulans: A reciprocal test of the *F ascertainment bias hypothesis. Mol. Biol. Evol. *F *F *F *F Primers 5' - 3' *F *F Forward *F *F Locus Repeat PCRSizeAnnealing Reverse *F (bp) temp. ( *F C) *F DSIM1 (GT)11 a e --- *F DSIM3 (TG)11 95 57 ttcacggcgatgtagacttg *F *F tgaggaaaggggtaagacga *F DSIM6a (GT)13 180 60 ccttggttgtcgtcgtcata *F *F ggcacgcatatccattctac *F DSIM6b (CT)6 180 60 tgctgttcttcccgtctctt *F *F ccaaaaaggaagcaggtgaa *F DSIM10 (GT)16CATC(TG)3 101 57 ttattgccctgctgctctctgct *F *F gatcgtgtgcattccacaga *F DSIM11 (CA)3CCAG(CA)11 b e --- *F DSIM13 (GT)11GA(GT)2 c e --- *F DSIM18 TGTA(TG)8 191 62 ttttgtcgatttttagggcg *F *F cgcggcactcttaacctatt *F DSIM25 (TG)12 199 62 tcgcatttccttggacattt *F *F gctccataaaggttttggca *F DSIM27 (TG)10TCTT(TG)3 110 e aaacaacattctttgcccgc *F *F tgaataatcaacaagccgca *F DSIM28a (TG)12GGTG 104 57 tgtttctggtttcctttggc *F *F ttcgattgtaaataccgcagc *F DSIM28b (TA)3GGTATG(TA)4GCG(TA)2 150 e gctgcggtatttacaatcgaa *F *F cagcaagcagcactagcaac *F DSIM29 (GT)11 151 62 attaagtatgcgcccgagtg *F *F cgcacaggtagttggtcctt *F DSIM30 (GT)17 182 60 tgaaatgaaatttatgccggt *F *F acaagcctttgacgggcatgtca *F DSIM35 compound 183 e taccacagccctaaggca *F *F cctcaacccatcttctacgt *F DSIM36 (TG)14 118 62 tgggtggcacattgtacttg *F *F atgcacgtagttggatggct *F DSIM42a (CA)7CG(CA)4TA(CA)2 217 e caataagcggcaaaacatgc *F *F tgtactgggaaaggacgagg *F DSIM42b (GT)12 130 60 cctcgtcctttcccagtaca *F *F cgccgaaaagcaagctaaatg *F DSIM43 compound 196 e cgtcatcgatttcagtggg *F *F caccgagcgactctaaacc *F DSIM44a compound 155 e ggttcaataacgcagcatga *F *F aaacccgtaaccccttgaac *F DSIM44b (CA)7 a e --- *F DSIM45 (TG)14 172 57 agaaaagtgcctgcattggt *F *F ggtaaaagtgatagtcgtttcca *F DSIM46 (CT)2CCGT(CT)15CC 146 e cgaagccggtttatttcaga *F *F gaagagaarggcgaaacagc *F DSIM47 (TG)12TATG 219 e ttcaggttgtgggctttagg *F *F acctattatggttgtgccgc *F DSIM48 compound 149 e cgtcagatgaagttgctcca *F *F ggcagccatctaccagcta *F DSIM49 compound 235 e ggcggtattagaactgttgc *F *F gtgtattgggttgtgagcct *F DSIM50 (GT)2CTCGTGTC(TG)16 138 62 a-gtaacgtggaacgcaagaca *F *F a-cgtgcacgaaacactcattt *F 130 e b-gacaaaagtcccagtgtctc *F *F b-aagtggagtgactgtgagtgga *F DSIM56a (CA)13 177 60 ttgcccggctaatacatttt *F *F cgcggcactcttaacctatt *F DSIM56b (AC)2AAAC(AT)7(AC)2AT(AC)4 103 e ggttgccgaggtactcacat *F *F aagggcaaagggagcaaaca *F DSIM57 (TG)6TA(TG)2(GT)5(TG)2 a e --- *F DSIM60 (TG)2TC(TG)2TATCG(GT)9 a e --- *F DSIM76 (CA)2TA(CA)5TA(CA)7 139 e gagagcttggggcagattta *F *F aactgcatgttacagcggc *F DSIM77 (TC)2(TG)5(TT)2(TG)8 c e --- *F DSIM86 (GT)10 142 62 ggcactcgacgacaacaac *F *F ttttgtaccgtttcggttcc *F DSIM96 (TG)2AG(TG)10GA(TG)2 c e --- *F DSIM97 (CA)11(CTCA)2 184 e ctgcagttctctggtgggta *F *F tgcagcaagcgaattagatg *F DSIM98 (TG)8(GT)3CT(GT)5 151 e atttcatggttttccctccc *F *F ctgtggcttaaaggccatgt *F DSIM99 (TG)2CG(TG)10(TGG)2 132 e cgccgtcaatatgattttcc *F *F gaactgcattgggaaacgat *F DSIM100 (TG)10TT(TG)3 141 e ggactgctctggttttc *F *F attatgggaaaggtcaggcg *F DSIM103 (CA)12 149 60 gccacactactcctacccca *F *F gagccgaggacagagacatc *F DSIM106 AG(TG)3(AG)6AT(AG)7 113 e ggaataatcctctactcatcaggc *F *F cataccgcctaaactaagccc *F DSIM111 (TG)15 183 e attagtgcaaacggcagtgg *F *F tggcatgagcacaatcaaat *F DSIM118 (CT)13TT(CT)3(AT)2(CT)7 b e --- *F DSIM119 (TG)11 132 62 ccgctcattatttacacacgg *F *F tcgacgtttacatcagcgac *F DSIM122 compound a e --- *F DSIM127 (TG)11 188 e gcccggagatatgtgaaatc *F *F attcggaggcagtgagtcag *F DSIM144 TG(GT)2(TG)14 211 e atttttaccgaaaccccctg *F *F tggtaaaagtgatagtcgtttcca *F DSIM145 (AC)4AG(AC)4CC(AC)6GC(AC)6 144 e gcgggtctcagttttgttgt *F *F tagtcagcgttgtttgcacc *F DSIM149 TG(TC)2(TG)14 173 e ggaaacgtaagctgtcgtc *F *F atgctcgtagttggatggct *F DSIM151 (CA)4(AC)2CT(CA)12 a e --- *F DSIM154 (TG)10TATG 161 59 gcgcttccttcaacctttgt *F *F gcccatgcagaagagttcaag *F DSIM155a (GA)5 194 e cccataagcgctatctctgc *F *F agtccggggtcttgagtttt *F DSIM155b compound 145 e cttcagaactcaagacccgc *F *F cagagtctcaggccgtcaat *F DSIM160 TGC(TG)5GG(TG)8GGTG 142 e gtaagcggccgtaacatttc *F *F gccggaatacatatgcaaaa *F DSIM192 (TG)2AG(TG)9 b e --- *F *F a) Primers were not able to be designed because the microsatellite was too *F close to the cloning site of the insert. *F *F b) Primers were not able to be designed because the flanking sequence was *F either too A/T rich or harbored too many other repeat sequences. *F *F c) Primers were not able to be designed because the sequence for the clone *F was not of sufficient quality. *F *F d) 'compound' refers to loci where either two microsatellite repeats are *F adjacent to one another or the repeat unit was a combination of repeat *F motifs. *F *F e) Annealing temperature not determined. # *U FBrf0112120 *a Cornell lab *b ?. *t 1999.11.30 *T personal communication to FlyBase *u *F http://www.mbg.cornell.edu/aquadro/microsatellites/correct.html *F *F Corrections to Table 1a *F *F ------------------------------------------------------------------------ *F *F Cytological location of DROMHC listed as 60E9 in Table 1. Correct *F cytological location of DROMHC is 36A7-C1. *F *F Cytological location of DROEXO2 listed as 16C-18B in Table 1. Correct *F cytological location of DROEXO2 is 13F. *F *F ------------------------------------------------------------------------ *F *F a Schug, M.D., K.A. Wetterstrand, M.S. Gaudette, R.H. Lim, C.M. Hutter and *F C.F. Aquadro. 1998. The distribution and frequency of microsatellite loci in *F Drosophila melanogaster. Molecular Ecology 7:57-70. *F *F ------------------------------------------------------------------------ *F *F Last Updated 3/8/98 *F *F Back to The Aquadro Lab # *U FBrf0112121 *a UCL lab *b ?. *t 1999.10.10 *T personal communication to FlyBase *u *F http://www.ucl.ac.uk/biology/goldstein/mlist1.htm *F *F 10 10 99 *F *F Chromosome X: *F *F locus name: AF047180 *F accession number: AF047180 *F cytogenetic position: 1B8 *F genetic position: 1-0 *F microsatellite repeat *F [position]: (AT)16 [1695] *F forward primer sequence *F [position]: taccttaggaaacccgaccc [1519] *F reverse primer sequence *F [position]: tcttgttgcgaattttgttca [1787] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.84; sim = 0.00; sec = *F 0.00 *F fragment length (bp): 317 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMZW3K25 *F accession number: X54006 *F cytogenetic position: 3B1 *F genetic position: 1-1.3 *F microsatellite repeat *F [position]: (AT)14 [3068] *F forward primer sequence *F [position]: attgtcattttattgctgccg [3040] *F reverse primer sequence *F [position]: taacgaagagagttgcggaga [3142] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 103 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DMC114E2 *F accession number: Z98254 *F cytogenetic position: 3D1-E8 *F genetic position: 1-3.5 *F microsatellite repeat *F [position]: (AT)17 [377] *F forward primer sequence *F [position]: caactgcagcagcaacaaat [145] *F reverse primer sequence *F [position]: attcgtaagttgcccgtctg [473] *F amplification *F properties: sim/sec - no amplification *F maps to clone: 114E2 *F heterozygosity: mel = 0.76 *F fragment length (bp): 329 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DMSEG0007* *F accession number: AL033125 *F cytogenetic position: 4A4-B2 *F genetic position: 1-6.5 *F microsatellite repeat *F [position]: (GT)10 [15803] *F forward primer sequence *F [position]: gagtcaacgagccagcaaagt [15843] *F reverse primer sequence *F [position]: aacaatacagagcagcacacg [15724] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 120 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DMU56661 *F accession number: U56661 *F cytogenetic position: 4F1-F2 *F genetic position: 1-11 *F microsatellite repeat *F [position]: (AC)15 [1098] *F forward primer sequence *F [position]: tatttcgctaacaaaccggc [914] *F reverse primer sequence *F [position]: aacgcgatcacaaacatcaa [1190] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.76; sim = 0.48; sec = *F 0.56 *F fragment length (bp): 277 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DROACT5CA *F accession number: M32055 *F cytogenetic position: 5C2-C5 *F *F genetic position: (TG)5 *F [211] *F microsatellite repeat *F [position]: (TG)5 [211] *F forward primer sequence *F [position]: tatacacacactcacgcgca [171] *F reverse primer sequence *F [position]: aaaccgactgaaagtggctg [459] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 289 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DMU09789 *F accession number: U09789 *F cytogenetic position: 6A1-A4 *F genetic position: 1-17 *F microsatellite repeat *F [position]: (AGTT)6 [2861] *F forward primer sequence *F [position]: acgcaataagttggcgta [2826] *F reverse primer sequence *F [position]: aatcaggataatgcctaat [2949] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 124 *F literature reference: modified from Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DRO12 *F accession number: D84425 *F cytogenetic position: 6F5 *F genetic position: 1-19.2 *F microsatellite repeat *F [position]: (CA)9 [428] *F forward primer sequence *F [position]: tcgggatctgcttagaaacaa [356] *F reverse primer sequence *F [position]: tgttgttgttggtactaggagttga [679] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: mel = 0.69 *F fragment length (bp): 324 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DMU96440 *F accession number: U96440 *F cytogenetic position: 7B3 *F genetic position: 1-20 *F microsatellite repeat *F [position]: (AG)14 [9269] *F forward primer sequence *F [position]: aatcttaacgcccagagggt [9242] *F reverse primer sequence *F [position]: ggttggaatgggatgatgac [9505] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: mel = 0.68; sim=0.00, sec=0.00 *F fragment length (bp): 264 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROOTUA *F accession number: M30825 *F cytogenetic position: 7F1 *F genetic position: 1-22.7 *F microsatellite repeat *F [position]: (TA)9 [5039] *F forward primer sequence *F [position]: tcacgaaatcaaagaaccacct [4950] *F reverse primer sequence *F [position]: gtctctagccgaacagcgag [5084] *F amplification *F properties: sec - no amplification *F maps to clone: - *F heterozygosity: sim = 0.00 *F fragment length (bp): 135 *F *F literature reference: modified from Goldstein & Clark *F 1995 *F date verified: 28.09.1999 *F *F locus name: G00630 *F accession number: G00630 *F cytogenetic position: 8B5-B8 *F genetic position: 1-25.5 *F microsatellite repeat *F [position]: (GT)12 [51] *F forward primer sequence *F [position]: gatcttttcatgtgttattt [1] *F reverse primer sequence *F [position]: ccgttttgtttggcaacttt [144] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 144 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004114 *F accession number: AC004114 *F cytogenetic position: 8E3-E4 *F genetic position: 1-28 *F microsatellite repeat *F [position]: (AC)13 [29018] *F forward primer sequence *F [position]: cacaaaggactcggcaagcac [28940] *F reverse primer sequence *F [position]: atcctccaaatgaaattacag [29107] *F amplification *F properties: fine *F maps to clone: DS05468 *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 168 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: AC004114 *F accession number: AC004114 *F cytogenetic position: 8E3-E4 *F genetic position: 1-28 *F microsatellite repeat *F [position]: (AC)14 [32429] *F forward primer sequence *F [position]: ttttattccagccatcaggc [32316] *F reverse primer sequence *F [position]: tgcggtcctttaccataagc [32535] *F amplification *F properties: fine *F maps to clone: DS05468 *F heterozygosity: mel = 0.51 *F fragment length (bp): 220 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROSEV *F accession number: J03158 *F cytogenetic position: 10A1-A2 *F genetic position: 1-33.4 *F microsatellite repeat *F [position]: (TTG)9 , (TA)7 [1888, 1721] *F forward primer sequence *F [position]: attaaagtgcaattaactat [1691] *F reverse primer sequence *F [position]: aagcgacaagtttcaattaac [1990] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.68; sim = 0.00; sec = *F 0.00 *F fragment length (bp): 300 *F literature reference: modified from Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DMTENA *F accession number: X68794 *F cytogenetic position: 11A6-A9 *F genetic position: 1-38 *F microsatellite repeat *F [position]: (AT)14 [3040] *F forward primer sequence *F [position]: ctcttagtgcgcagggattc [2999] *F reverse primer sequence *F [position]: tcaagagtcgctcaatggca [3101] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.56; sec = 0.00 *F fragment length (bp): 103 *F *F literature reference: modified from Goldstein & Clark *F 1995 *F date verified: 28.09.1999 *F *F locus name: DROYP3 *F accession number: M15898 *F cytogenetic position: 12B-C *F genetic position: 1-44 *F microsatellite repeat *F [position]: (ACG)6 [1720] *F forward primer sequence *F [position]: atccggctatttgcaatcaa [1603] *F reverse primer sequence *F [position]: tctggcttatatagctcc [1803] *F amplification *F properties: sim - no amplication *F maps to clone: - *F heterozygosity: mel = 0.44 *F fragment length (bp): 201 *F literature reference: modified from Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DMU18774 *F accession number: U18774 *F cytogenetic position: 13D *F genetic position: 1-51 *F microsatellite repeat *F [position]: (AC)12 [4395] *F forward primer sequence *F [position]: gatccttggcggcaggggagcgaa [4318] *F reverse primer sequence *F [position]: tatgcaactccttgcacaa [4508] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.63; sim = 0.64; sec = *F 0.48 *F fragment length (bp): 191 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DROEX02 *F accession number: L07660 *F cytogenetic position: 13F2 *F genetic position: 1-51.5 *F microsatellite repeat *F [position]: (CATA)8 [1963] *F forward primer sequence *F [position]: tgcagggcaccttctctcca [1882] *F reverse primer sequence *F [position]: gaacgcttgatttagatttggg [2016] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 135 *F literature reference: modified from Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DMRUDG5 *F accession number: X03879 *F cytogenetic position: 15A1 *F genetic position: 1-55 *F microsatellite repeat *F [position]: (AATT)5 [196] *F forward primer sequence *F [position]: tggaaccgtgttctatatgcc [171] *F reverse primer sequence *F [position]: tgaagatgtccagcagcg [319] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 149 *F literature reference: modified from Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DMARIADNE *F accession number: X98309 *F cytogenetic position: 16E2-17B1 *F genetic position: 1-58 *F microsatellite repeat *F [position]: (AC)14 [4943] *F forward primer sequence *F [position]: aacactgtccccatccacat [4896] *F reverse primer sequence *F [position]: tctgttcaactccttcggct [5021] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.66; sim = 0.32; sec = *F 0.56 *F fragment length (bp): 126 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMTROPONI *F accession number: X58188 *F cytogenetic position: 16F3-F6 *F genetic position: 1-57.6 *F microsatellite repeat *F [position]: (CA)11 [1231] *F forward primer sequence *F [position]: ccacagcgagaacaagagatccc [1193] *F reverse primer sequence *F [position]: tattttacgtgggcgacgtg [1319] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.69; sim = 0.56; sec = *F 0.00 *F fragment length (bp): 127 *F *F literature reference: modified from Goldstein & Clark *F 1995 *F date verified: 28.09.1999 *F *F locus name: DROPASSOV *F accession number: L13306 *F cytogenetic position: 19E3 *F genetic position: 1-64 *F microsatellite repeat *F [position]: (AT)14 [2590] *F forward primer sequence *F [position]: gtggaaatggcagaggagag [2418] *F reverse primer sequence *F [position]: gttgttcatttgtttagcgg [2675] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.48 *F fragment length (bp): 258 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AF017777 *F accession number: AF017777 *F cytogenetic position: 19F3-F6 *F genetic position: 1-65 *F microsatellite repeat *F [position]: (AT)14 [13656] *F forward primer sequence *F [position]: attagctaactccaagaacg [13537] *F reverse primer sequence *F [position]: aatcctctcagctcagcgta [13706] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.63; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 170 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F 10 10 99 *F *F Chromosome 2: *F *F locus name: AC004115 *F accession number: AC004115 *F cytogenetic position: 21B7-C2 *F genetic position: 2-0.7 *F microsatellite repeat *F [position]: (AG)17 [69178] *F forward primer sequence *F [position]: ttctgcccgcttaacctcta [69077] *F reverse primer sequence *F [position]: aagctaagtcagcatcccca [69261] *F amplification *F properties: fine *F maps to clone: DS02374 *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 185 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DROEXPAND *F accession number: L14768 *F cytogenetic position: 21C4 *F genetic position: 2-0.8 *F microsatellite repeat *F [position]: (CAG)8 [5140] *F forward primer sequence *F [position]: gtgatcgatcccgctgtc [5110] *F reverse primer sequence *F [position]: tccggtttccaattagcttg [5207] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 98 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: AC004441 *F accession number: AC004441 *F cytogenetic position: 22A3 *F genetic position: 2-3 *F microsatellite repeat *F [position]: (AT)20 [32238] *F forward primer sequence *F [position]: agaatacaaactcgattgcc [32139] *F reverse primer sequence *F [position]: tgaaagtcaaatgctggtgc [32328] *F amplification *F properties: sim/sec - no amplification *F maps to clone: DS00676 *F heterozygosity: mel = 0.85 *F fragment length (bp): 190 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROYANETSB *F accession number: M97694 *F cytogenetic position: 22D1-D2 *F genetic position: 2-5 *F microsatellite repeat *F [position]: (GT)20 [3625] *F forward primer sequence *F [position]: taatggggaatgggtgaatg [3596] *F reverse primer sequence *F [position]: gccgtgctctttctcttacg [3702] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 107 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DS01340 *F accession number: - *F cytogenetic position: 24A1-A2 *F genetic position: 2-10.5 *F microsatellite repeat *F [position]: (AG)9 *F forward primer sequence *F [position]: ggagcgcaatgctgtttaagt *F reverse primer sequence *F [position]: ggagtagtgcctgtctcggac *F amplification *F properties: sim - no amplification *F maps to clone: - *F heterozygosity: sec = 0.00 *F fragment length (bp): 179 *F literature reference: C. Schlotterer, unpublished *F date verified: 28.09.1999 *F *F locus name: AC005732 *F accession number: AC005732 *F cytogenetic position: 24C3-D2 *F genetic position: 2-12.5 *F microsatellite repeat *F [position]: (AC)13 [10245] *F forward primer sequence *F [position]: gaaaatccgaaatcggttga [10089] *F reverse primer sequence *F [position]: cttcttctctggctgctgct [10385] *F amplification *F properties: sim/sec - no amplification *F maps to clone: DS03998 *F heterozygosity: - *F fragment length (bp): 297 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC005732 *F accession number: AC005732 *F cytogenetic position: 24C3-D2 *F genetic position: 2-12.5 *F microsatellite repeat *F [position]: (TG)14 [20983] *F forward primer sequence *F [position]: taatttggcacaaaccacca [20875] *F reverse primer sequence *F [position]: gccgcataatggtcaaaagt [21113] *F amplification *F properties: fine *F maps to clone: DS03998 *F heterozygosity: sim = 0.72; sec = 0.00 *F fragment length (bp): 239 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: AC004373 *F accession number: AC004373 *F cytogenetic position: 24F1-F2 *F genetic position: 2-12.5 *F microsatellite repeat *F [position]: (AT)15 [56821] *F forward primer sequence *F [position]: aatgcgtgtgtttggatgaa [56770] *F reverse primer sequence *F [position]: gtcccagtctcccagtgaaa [56958] *F amplification *F properties: fine *F maps to clone: DS05273 *F *F heterozygosity: mel = 0.77; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 189 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004721 *F accession number: AC004721 *F cytogenetic position: 25E6 *F genetic position: 2-17.5 *F microsatellite repeat *F [position]: (CA)8 [60694] *F forward primer sequence *F [position]: caagcctctctttaccgcac [60667] *F reverse primer sequence *F [position]: agtttttgctgggtcccttt [60772] *F amplification *F properties: sim/sec - no amplification *F maps to clone: DS03308 *F heterozygosity: mel = 0.75 *F fragment length (bp): 106 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROGPDHA *F accession number: J04567 *F cytogenetic position: 26A1 *F genetic position: 2-18 *F microsatellite repeat *F [position]: (CT)7 [1228] *F forward primer sequence *F [position]: cattggaaaagtgagcggat [1137] *F reverse primer sequence *F [position]: ttggtttgcactccacacat [1315] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.32; sec = 0.00 *F fragment length (bp): 179 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004758 *F accession number: AC004758 *F cytogenetic position: 26A5-B5 *F genetic position: 2-18 *F microsatellite repeat *F [position]: (TG)11 [14703] *F forward primer sequence *F [position]: tgctttcgctttcggtatct [14620] *F reverse primer sequence *F [position]: aacggagtgcctatgcatt [14821] *F amplification *F properties: fine *F maps to clone: DS06106 *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 202 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC003052 *F accession number: AC003052 *F cytogenetic position: 27A2-B2 *F genetic position: 2-22 *F microsatellite repeat *F [position]: (AT)23 [6477] *F forward primer sequence *F [position]: atcgtcgaacgagaccgta [6404] *F reverse primer sequence *F [position]: tcgatttaattgcggtgtga [6691] *F amplification *F properties: fine *F maps to clone: DS06905 *F *F heterozygosity: mel = 0.82; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 288 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DRONINAC *F accession number: J03131 *F cytogenetic position: 28A1-A3 *F genetic position: 2-22 *F microsatellite repeat *F [position]: (AT)10 [5673] *F forward primer sequence *F [position]: tttgtcaatctctcacagcagg [5622] *F reverse primer sequence *F [position]: gcccgagtacatttattcaagc [5760] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 139 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: AC005555 *F accession number: AC005555 *F cytogenetic position: 29A1-C1 *F genetic position: 2-31.5 *F microsatellite repeat *F [position]: (TG)12 [46209] *F forward primer sequence *F [position]: ggttgctgggagaaagac [46086] *F reverse primer sequence *F [position]: gccacacattcgcatctc [46256] *F amplification *F properties: fine *F maps to clone: DS09038 *F *F heterozygosity: mel = 0.61; sim = 0.72; sec = *F 0.00 *F fragment length (bp): 171 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMBIBGENE *F accession number: X53275 *F cytogenetic position: 30F *F genetic position: 2-34.7 *F microsatellite repeat *F [position]: (CAG)5 [2293] *F forward primer sequence *F [position]: tcgcaaggatcagcggtgac [2248] *F reverse primer sequence *F [position]: ttgggcctcagcggcagcat [2399] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.32; sec = 0.00 *F fragment length (bp): 152 *F *F literature reference: modified from Michalakis & *F Veuille 1996 *F date verified: 28.09.1999 *F *F locus name: DMU12269 *F accession number: U12269 *F cytogenetic position: 31A1-A3 *F genetic position: 2-39 *F microsatellite repeat *F [position]: (AAC)7 [2415] *F forward primer sequence *F [position]: tgggatccgtggatcatagt [2234] *F reverse primer sequence *F [position]: attcgggaatgaggacactg [2470] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: mel = 0.78 *F fragment length (bp): 237 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DRODANS *F accession number: J03148 *F cytogenetic position: 31E *F genetic position: 2-41.3 *F microsatellite repeat *F [position]: (CAG)5 [2229] *F forward primer sequence *F [position]: tgcccagcatcacatgatac [2063] *F reverse primer sequence *F [position]: ggtttttatggaagagaggg [2342] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.32 *F fragment length (bp): 280 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC005117 *F accession number: AC005117 *F cytogenetic position: 32D1-D2 *F genetic position: 2-44.2 *F microsatellite repeat *F [position]: (CA)11 [32149] *F forward primer sequence *F [position]: agtgaagggatccgcactaa [31958] *F reverse primer sequence *F [position]: tgccacttgaactctgacca [32216] *F amplification *F properties: sim/sec - not tested *F maps to clone: DS04313 *F heterozygosity: - *F fragment length (bp): 259 *F literature reference: J. Gockel, unpublished *F date verified: 28.09.1999 *F *F locus name: G410 *F accession number: - *F cytogenetic position: 33E9-E10 *F genetic position: 2-47 *F microsatellite repeat *F [position]: (CT)11 *F forward primer sequence *F [position]: ttcggctctttgtttgcttg *F reverse primer sequence *F [position]: aagcttaaaccgatcgaaaac *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.32 *F fragment length (bp): 150 *F literature reference: C. Schlotterer, unpublished *F date verified: 28.09.1999 *F *F locus name: AC006302 *F accession number: AC006302 *F cytogenetic position: 34C4-D2 *F genetic position: 2-48.5 *F microsatellite repeat *F [position]: (AAT)11 [74562] *F forward primer sequence *F [position]: tgttttccatgccagctagt [74529] *F reverse primer sequence *F [position]: gcccggaaaattcttgttta [74780] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.71; sim = 0.64; sec = *F 0.32 *F fragment length (bp): 252 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004118 *F accession number: AC004118 *F cytogenetic position: 35B2-B3 *F genetic position: 2-50.6 *F microsatellite repeat *F [position]: (CT)15 [21425] *F forward primer sequence *F [position]: ccaacttgggcgagagaatt [21282] *F reverse primer sequence *F [position]: gcttaattgcctcactgtgc [21570] *F amplification *F properties: fine *F maps to clone: DS06238 *F *F heterozygosity: mel = 0.83; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 289 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: L49403 *F accession number: L49403 *F cytogenetic position: 35B2-B10 *F genetic position: 2-50.9 *F microsatellite repeat *F [position]: (AT)19 [26138] *F forward primer sequence *F [position]: aaacttgaggggttgattcat [26075] *F reverse primer sequence *F [position]: taaatggagcaaagatacat [26355] *F amplification *F properties: sim/sec - no amplification *F maps to clone: DS07721 *F heterozygosity: mel = 0.80 *F fragment length (bp): 281 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROMHC *F accession number: M61229 *F cytogenetic position: 36B1 *F genetic position: 2-52 *F microsatellite repeat *F [position]: (CA)13 [7135] *F forward primer sequence *F [position]: aaacccacacaacaactgca [7108] *F reverse primer sequence *F [position]: acattaccgatattggatgca [7215] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.72; sec = 0.00 *F fragment length (bp): 108 *F *F literature reference: modified from Goldstein & Clark *F 1995 *F date verified: 28.09.1999 *F *F locus name: DRODORSAL *F accession number: M23702 *F cytogenetic position: 36C *F genetic position: 2-52.9 *F microsatellite repeat *F [position]: (CAG)9 [1698] *F forward primer sequence *F [position]: ctgatgtcgcccaaccatcc [1533] *F reverse primer sequence *F [position]: ccgccaggattgccaaaggg [1777] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.44; sec = 0.32 *F fragment length (bp): 245 *F literature reference: Michalakis & Veuille 1996 *F date verified: 28.09.1999 *F *F locus name: AC002474 *F accession number: AC002474 *F cytogenetic position: 38D1-D2 *F genetic position: 2-54.2 *F microsatellite repeat *F [position]: (CA)13 [29169] *F forward primer sequence *F [position]: gatgctgtccttcggacttc [29046] *F reverse primer sequence *F [position]: aacaacaaagcccattctgc [29291] *F amplification *F properties: fine *F maps to clone: DS05709 *F heterozygosity: sim = 0.00; sec = 0.48 *F fragment length (bp): 246 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004759 *F accession number: AC004759 *F cytogenetic position: 38E1-E9 *F genetic position: 2-54.3 *F microsatellite repeat *F [position]: (CCT)11 [42152] *F forward primer sequence *F [position]: acagacggaaagccaaaatg [42006] *F reverse primer sequence *F [position]: cactccgcctcgtttcttac [42246] *F amplification *F properties: fine *F maps to clone: DS04217 *F *F heterozygosity: mel = 0.70; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 241 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC006472 *F accession number: AC006472 *F cytogenetic position: 45E1-46A2 *F genetic position: 2-61 *F microsatellite repeat *F [position]: (AG)16 [82287] *F forward primer sequence *F [position]: tcctccatgtaaagataaacgct [82235] *F reverse primer sequence *F [position]: aactcgcaaattgcctaacg [82531] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.67; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 297 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC005974* *F accession number: AC005974 *F cytogenetic position: 46E1-E9 *F genetic position: 2-62.1 *F microsatellite repeat *F [position]: (CT)9 [117669] *F forward primer sequence *F [position]: ttaagcgaactgctccgaat [117799] *F reverse primer sequence *F [position]: tgatttttgccagttcagca [117609] *F amplification *F properties: sim/sec - not tested *F maps to clone: DS05033 *F heterozygosity: mel = 0.72 *F fragment length (bp): 191 *F literature reference: J. Gockel, unpublished *F date verified: 28.09.1999 *F *F locus name: DM1433 *F accession number: Y12573 *F cytogenetic position: 46E4 *F genetic position: 2-61 *F microsatellite repeat *F [position]: (AG)11 [2647] *F forward primer sequence *F [position]: gcttgaagaatccctgcttg [2486] *F reverse primer sequence *F [position]: tgatttttgccagttcagca [2728] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.48; sec = 0.32 *F fragment length (bp): 243 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DROGPAD *F accession number: M31129 *F cytogenetic position: 47A *F genetic position: 2-60 *F microsatellite repeat *F [position]: (GT)9 [783] *F forward primer sequence *F [position]: gaaataggaatcattttgaatggc [699] *F reverse primer sequence *F [position]: aattaaaaacaaaaaacctgagcg [883] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.32; sec = 0.00 *F fragment length (bp): 185 *F literature reference: Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DMMP20 *F accession number: Y00795 *F cytogenetic position: 49F9-F13 *F genetic position: 2-68 *F microsatellite repeat *F [position]: (CA)10 [656] *F forward primer sequence *F [position]: catgcaaatgagcagtactttg [610] *F reverse primer sequence *F [position]: tattttcacacatttccaatcg [703] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.69; sim = 0.32; sec = *F 0.00 *F fragment length (bp): 94 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DMU19731 *F accession number: U19731 *F cytogenetic position: 51A1-A2 *F genetic position: 2-71 *F microsatellite repeat *F [position]: (TG)9 [4619] *F forward primer sequence *F [position]: acctttttctcgcagtgcat [4574] *F reverse primer sequence *F [position]: attgtggctggctgtttacc [4755] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.56; sim = 0.44; sec = *F 0.00 *F fragment length (bp): 182 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004306* *F accession number: AC004306 *F cytogenetic position: 51E1-E2 *F genetic position: 2-74 *F microsatellite repeat *F [position]: (GT)9 [54993] *F forward primer sequence *F [position]: gagacaccccttgacgagtg [55089] *F reverse primer sequence *F [position]: ctcaaaacaaacccagtctc [54966] *F amplification *F properties: fine *F maps to clone: DS01386 *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 124 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004516 *F accession number: AC004516 *F cytogenetic position: 52D2-D15 *F genetic position: 2-76 *F microsatellite repeat *F [position]: (AC)18 [17036] *F forward primer sequence *F [position]: tcgtcgcccgttaatata [16909] *F reverse primer sequence *F [position]: accgttcgtgggtcaaatag [17181] *F amplification *F properties: fine *F maps to clone: DS08018 *F *F heterozygosity: mel = 0.84; sim = 0.44; sec = *F 0.00 *F fragment length (bp): 273 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004248 *F accession number: AC004248 *F cytogenetic position: 52D2-D15 *F genetic position: 2-77 *F microsatellite repeat *F [position]: (CA)22 [76471] *F forward primer sequence *F [position]: caatttccctcgcactgacac [76435] *F reverse primer sequence *F [position]: cggaaacgaacgggcgataag [76600] *F amplification *F properties: sec - null alleles present *F maps to clone: DS03910 *F heterozygosity: sim = 0.56; sec = 0.8 *F fragment length (bp): 166 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004641 *F accession number: AC004641 *F cytogenetic position: 53D1-E2 *F genetic position: 2-81 *F microsatellite repeat *F [position]: (AC)22 [122708] *F forward primer sequence *F [position]: tcaagtagggggtgtcgttc [122538] *F reverse primer sequence *F [position]: aaccaacaactaattgcggc [122784] *F amplification *F properties: fine *F maps to clone: DS07321 *F *F heterozygosity: mel = 0.89; sim = 0.48; sec = *F 0.00 *F fragment length (bp): 247 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DS00361 *F accession number: - *F cytogenetic position: 54B1-B2 *F genetic position: 2-82 *F microsatellite repeat *F [position]: (AC)8 *F forward primer sequence *F [position]: caaccacccacaagcacac *F reverse primer sequence *F [position]: cctctccggttgggctac *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.68; sec = 0.00 *F fragment length (bp): 140 *F *F literature reference: modified from C. Schlotterer, *F unpublished *F date verified: 28.09.1999 *F *F locus name: AC004640 *F accession number: AC004640 *F cytogenetic position: 54F1-55A *F genetic position: 2-84.5 *F microsatellite repeat *F [position]: (AT)13 [16238] *F forward primer sequence *F [position]: ccgtaagcccataagcgtaa [16171] *F reverse primer sequence *F [position]: ggctacggctagagttcgtg [16427] *F amplification *F properties: fine *F maps to clone: DS07528 *F *F heterozygosity: mel = 0.70; sim = 0.56; sec = *F 0.00 *F fragment length (bp): 257 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMELF1 *F accession number: X15657 *F cytogenetic position: 55A1 *F genetic position: 2-86 *F microsatellite repeat *F [position]: (CAA)7 [317] *F forward primer sequence *F [position]: tgtaagcaaaggcccagagag [274] *F reverse primer sequence *F [position]: gagtctggagctgtaactgaa [451] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.56; sec = 0.48 *F fragment length (bp): 178 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: AC004307 *F accession number: AC004307 *F cytogenetic position: 56D11-E6 *F genetic position: 2-90 *F microsatellite repeat *F [position]: (AT)14 [29273] *F forward primer sequence *F [position]: atgtagtgcgagattcggct [29154] *F reverse primer sequence *F [position]: ggctgcactctacaaacgct [29397] *F amplification *F properties: fine *F maps to clone: DS02074 *F *F heterozygosity: mel = 0.74; sim = 0.00; sec = *F 0.00 *F fragment length (bp): 244 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DS08687a *F accession number: - *F cytogenetic position: 57C5-D1 *F genetic position: 2-93.5 *F microsatellite repeat *F [position]: (GT)11 *F forward primer sequence *F [position]: tgatttggactgagatcagg *F reverse primer sequence *F [position]: gcccaacgaatcatttcac *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.44; sec = 0.00 *F fragment length (bp): 184 *F literature reference: C. Schlotterer, unpublished *F date verified: 28.09.1999 *F *F locus name: AC004564 *F accession number: AC004564 *F cytogenetic position: 57E1-E2 *F genetic position: 2-95 *F microsatellite repeat *F [position]: (GT)12 [57223] *F forward primer sequence *F [position]: gaatttcaaaatgggcgaaa [57140] *F reverse primer sequence *F [position]: attcacgtgctatgtgcagc [57273] *F amplification *F properties: fine *F maps to clone: DS08012 *F *F heterozygosity: mel = 0.75; sim = 0.00; sec = *F 0.00 *F fragment length (bp): 134 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004365 *F accession number: AC004365 *F cytogenetic position: 58A4-B1 *F genetic position: 2-97.5 *F microsatellite repeat *F [position]: (AT)17 [55955] *F forward primer sequence *F [position]: gctttatcaatgcagcctcc [55867] *F reverse primer sequence *F [position]: ggccccaatatgtcctcgcc [56085] *F amplification *F properties: fine *F maps to clone: DS00642 *F *F heterozygosity: mel = 0.66; sim = 0.48; sec = *F 0.00 *F fragment length (bp): 219 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DS08011 *F accession number: - *F cytogenetic position: 59A1-B2 *F genetic position: 2-101 *F microsatellite repeat *F [position]: (GT)8 *F forward primer sequence *F [position]: agccacagccatgcgtttaac *F reverse primer sequence *F [position]: cacacgctgacaggatctact *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 107 *F literature reference: C. Schlotterer, unpublished *F date verified: 28.09.1999 *F *F locus name: DM92D10S *F accession number: Z50696 *F cytogenetic position: 59A1-B8 *F genetic position: 2-102 *F microsatellite repeat *F [position]: (AT)19 [46] *F forward primer sequence *F [position]: ccgaatcgatgtagttccttg [1] *F reverse primer sequence *F [position]: aaggctccggtccttgttag [155] *F amplification *F properties: fine *F maps to clone: 92D10 *F *F heterozygosity: mel = 0.86; sim = 0.48; sec = *F 0.48 *F fragment length (bp): 155 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F 10 10 99 *F *F Chromosome 3: *F *F locus name: DMU42699 *F accession number: U42699 *F cytogenetic position: 61C1-C2 *F genetic position: 3-1 *F microsatellite repeat *F [position]: (ACC)8 [3570] *F forward primer sequence *F [position]: taggtggtagacgaccaggg [3521] *F reverse primer sequence *F [position]: cactggatcccgctgatc [3623] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 103 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: AC004343 *F accession number: AC004343 *F cytogenetic position: 62A1-A2 *F genetic position: 3-0.5 *F microsatellite repeat *F [position]: (TC)12 [19883] *F forward primer sequence *F [position]: acggtaattgcggatgagac [19741] *F reverse primer sequence *F [position]: acgatggcaacaaggatctc [19962] *F amplification *F properties: sim - null alleles present *F maps to clone: DS02734 *F *F heterozygosity: mel = 0.73; sim = 0.72; sec = *F 0.00 *F fragment length (bp): 222 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMRHO *F accession number: X52454 *F cytogenetic position: 62A1-A3 *F genetic position: 3-0.1 *F microsatellite repeat *F [position]: (AC)10 [2273] *F forward primer sequence tatactaaagtcacttaatgcgttaca *F [position]: [2217] *F reverse primer sequence gtttgtgtgaatttcactaaattatta *F [position]: [2411] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.56; sec = 0.64 *F fragment length (bp): 195 *F literature reference: Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DROCDPR *F accession number: L32839 *F cytogenetic position: 62B11 *F genetic position: 3-1.5 *F microsatellite repeat *F [position]: (ACC)5 [939] *F forward primer sequence *F [position]: tatcctatgcaaacacaggcc [762] *F reverse primer sequence *F [position]: ggccataactgaaaagctatgc [1133] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 372 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DMHSP82 *F accession number: X03810 *F cytogenetic position: 63B3-B11 *F genetic position: 3-5 *F microsatellite repeat *F [position]: (TA)5 [4632] *F forward primer sequence *F [position]: actaaagactacatacgcgccc [4581] *F reverse primer sequence *F [position]: gagtgtccacggtggagg [4809] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 229 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: AC004658 *F accession number: AC004658 *F cytogenetic position: 63D2-E1 *F genetic position: 3-8 *F microsatellite repeat *F [position]: (AC)12 [87788] *F forward primer sequence *F [position]: atttggtccacgagagattt [87757] *F reverse primer sequence *F [position]: tgggaaaactgtgccacata [87878] *F amplification *F properties: fine *F maps to clone: DS00079 *F *F heterozygosity: mel = 0.71; sim = 0.56; sec = *F 0.48 *F fragment length (bp): 122 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMU36477 *F accession number: U36477 *F cytogenetic position: 64A *F genetic position: 3-10 *F microsatellite repeat *F [position]: (AT)14 [359] *F forward primer sequence *F [position]: cggcgagccaaactcttat [334] *F reverse primer sequence *F [position]: attatttgtggcaaaagcgg [454] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.48; sec = 0.32 *F fragment length (bp): 121 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DRODSRC *F accession number: K01043 *F cytogenetic position: 64B *F genetic position: 3-15 *F microsatellite repeat *F [position]: (TA)7 [1051] *F forward primer sequence *F [position]: gacaacatttatcagctgctgc [854] *F reverse primer sequence *F [position]: agagttcatgtgtgttggttgg [1172] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.56 *F fragment length (bp): 319 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: AC004767 *F accession number: AC004767 *F cytogenetic position: 65A6-B2 *F genetic position: 3-16.5 *F microsatellite repeat *F [position]: (TA)14 [149421] *F forward primer sequence *F [position]: attcacgcaacgtttagaat [149314] *F reverse primer sequence *F [position]: attttggcattgcttttgct [149620] *F amplification *F properties: sim/sec - no amplification *F maps to clone: DS01986 *F heterozygosity: mel = 0.80 *F fragment length (bp): 307 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: AC004352 *F accession number: AC004352 *F cytogenetic position: 65C5-D1 *F genetic position: 3-18 *F microsatellite repeat *F [position]: (AG)14 [66500] *F forward primer sequence *F [position]: tcctcggtgagaccgtaatc [66293] *F reverse primer sequence *F [position]: gggcagaggaaaagcactca [66583] *F amplification *F properties: fine *F maps to clone: DS02898 *F heterozygosity: sim = 0.44; sec = 0.00 *F fragment length (bp): 291 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMU14395 *F accession number: U14395 *F cytogenetic position: 65D1-D3 *F genetic position: 3-18 *F microsatellite repeat *F [position]: (TC)12 [2468] *F forward primer sequence *F [position]: gggcagaggaaaagcactca [2412] *F reverse primer sequence *F [position]: tcggtgagaccgtaatctgc [2697] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.72; sim = 0.8; sec = *F 0.32 *F fragment length (bp): 286 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC004351 *F accession number: AC004351 *F cytogenetic position: 65D4-E1 *F genetic position: 3-18.5 *F microsatellite repeat *F [position]: (CA)10 [29968] *F forward primer sequence *F [position]: tggaggcgtctctgaacttt [29843] *F reverse primer sequence *F [position]: ccaaattcggctaattgcat [30094] *F amplification *F properties: sim/sec - not tested *F maps to clone: DS06194 *F heterozygosity: - *F fragment length (bp): 252 *F literature reference: J. Gockel, unpublished *F date verified: 28.09.1999 *F *F locus name: DROHSP1 *F accession number: M26267 *F cytogenetic position: 67B1 *F genetic position: 3-35 *F microsatellite repeat *F [position]: (CAGC)5 [473] *F forward primer sequence *F [position]: tttgtagcttccaggcgg [451] *F reverse primer sequence *F [position]: ctgaatttccactgccgg [599] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 149 *F literature reference: Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DROLAMB2A *F accession number: M58417 *F cytogenetic position: 67C *F genetic position: 3-28 *F microsatellite repeat *F [position]: (ATT)5 [4925] *F forward primer sequence *F [position]: cgtaggaaggaaagaaatcgg [4774] *F reverse primer sequence *F [position]: aatttgcagttgataggcagc [5001] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.72; sec = 0.00 *F fragment length (bp): 228 *F literature reference: modified from Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DROGTPAAP *F accession number: M86655 *F cytogenetic position: 67D2-D3 *F genetic position: 3-32 *F microsatellite repeat *F [position]: (CAG)5 [3564] *F forward primer sequence *F [position]: ctgaaatacggcagcagaca [3444] *F reverse primer sequence *F [position]: taggcgttcatgttgctcgt [3673] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.48; sec = 0.00 *F fragment length (bp): 230 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DROECT *F accession number: M14740 *F cytogenetic position: 67D8-D10 *F genetic position: 3-38 *F microsatellite repeat *F [position]: (ATTT)9 [760] *F forward primer sequence *F [position]: gccgttttaggtcatctaat [728] *F reverse primer sequence *F [position]: ttactgtaatgacggaacag [1058] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 331 *F literature reference: modified from Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DROECT *F accession number: M14740 *F cytogenetic position: 67D8-D10 *F genetic position: 3-38 *F microsatellite repeat *F [position]: (ATTT)9 [760] *F forward primer sequence gaagaaatagagaattgttaaacctt *F [position]: [701] *F reverse primer sequence *F [position]: ctaatgatgagtgtgagt [1035] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 335 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: AC004438 *F accession number: AC004438 *F cytogenetic position: 68C2-C4 *F genetic position: 3-36.2 *F microsatellite repeat *F [position]: (GACT)6 [59760] *F forward primer sequence *F [position]: tgataaagggaccgaaacca [59698] *F reverse primer sequence *F [position]: cagttcgggtgcggtatagt [59979] *F amplification *F properties: sim/sec - not tested *F maps to clone: DS03786 *F heterozygosity: - *F fragment length (bp): 282 *F literature reference: J. Gockel, unpublished *F date verified: 28.09.1999 *F *F locus name: DMZ60MEX1 *F accession number: X58286 *F cytogenetic position: 71C3-D2 *F genetic position: 3-43 *F microsatellite repeat *F [position]: (TTC)8 [3348] *F forward primer sequence *F [position]: aaatctgttgctcatactgccc [3325] *F reverse primer sequence *F [position]: aaccggcgaaatgttcag [3419] *F amplification *F properties: sec - null alleles present *F maps to clone: - *F heterozygosity: sim = 0.00 *F fragment length (bp): 95 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DM22F11T *F accession number: Z83456 *F cytogenetic position: 73A1-B7 *F genetic position: 3-44 *F microsatellite repeat *F [position]: (AT)22 [181] *F forward primer sequence *F [position]: ggatgctcggataccaaaaa [67] *F reverse primer sequence *F [position]: tcgcctgtgacttagattgc [280] *F amplification *F properties: fine *F maps to clone: 22F11 *F *F heterozygosity: mel = 0.78; sim = 0.72; sec = *F 0.72 *F fragment length (bp): 214 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMCATHPO *F accession number: X52286 *F cytogenetic position: 75D4 *F genetic position: 3-47 *F microsatellite repeat *F [position]: (ACC)6 [1694] *F forward primer sequence *F [position]: ttcgacggatcagacttggttttt [1622] *F reverse primer sequence gcgttcgcctttcttagtcaatttcgg *F [position]: [1751] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.72; sec = 0.00 *F fragment length (bp): 130 *F literature reference: modified from Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DMU43583 *F accession number: U43583 *F cytogenetic position: 83A3-A5 *F genetic position: 3-46.5 *F microsatellite repeat *F [position]: (AT)22 [4112] *F forward primer sequence *F [position]: cgttaatcaggtgagcaat [4062] *F reverse primer sequence *F [position]: ggatctgtaaaactgccaagtg [4340] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: mel = 0.76 *F fragment length (bp): 279 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: AC001655 *F accession number: AC001655 *F cytogenetic position: 84C1-C4 *F genetic position: 3-47 *F microsatellite repeat *F [position]: (AC)12 [11835] *F forward primer sequence *F [position]: tcccacaaccaaataccgtt [11621] *F reverse primer sequence *F [position]: ggttccccttttcatccatt [11893] *F amplification *F properties: fine *F maps to clone: DS07700 *F heterozygosity: sim = 0.56; sec = 0.32 *F fragment length (bp): 273 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: AC001655 *F accession number: AC001655 *F cytogenetic position: 84C1-C4 *F genetic position: 3-47 *F microsatellite repeat *F [position]: (AC)9 [44111] *F forward primer sequence *F [position]: ttcgtttttgaattattgcgc [44006] *F reverse primer sequence *F [position]: cctgttcgtccaactatgtgc [44149] *F amplification *F properties: fine *F maps to clone: DS07700 *F heterozygosity: - *F fragment length (bp): 144 *F literature reference: Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DROPROSA *F accession number: D10609 *F cytogenetic position: 86E3 *F genetic position: 3-51 *F microsatellite repeat *F [position]: (CA)6, (GA)12 [5296, 5339] *F forward primer sequence *F [position]: caataaccacacgcattcca [5268] *F reverse primer sequence *F [position]: aaccacttcctgtttggcc [5391] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.60; sim = 0.56; sec = *F 0.00 *F fragment length (bp): 124 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMTRXIII *F accession number: Z50152 *F cytogenetic position: 88B3 *F genetic position: 3-54.2 *F microsatellite repeat *F [position]: (CT)23 [6913] *F forward primer sequence *F [position]: tttaccttttgcgcttgctt [6834] *F reverse primer sequence *F [position]: agacaatcggccaacaaaac [7149] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: mel = 0.91 *F fragment length (bp): 316 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DMTRXIII2 *F accession number: Z50152 *F cytogenetic position: 88B3 *F genetic position: 3-54.2 *F microsatellite repeat *F [position]: (AG)10 [10941] *F forward primer sequence *F [position]: acaaaagccgaacgagaaaa [10803] *F reverse primer sequence *F [position]: ctgctgcgttgtggctcctt [11083] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 281 *F literature reference: S. J. Macdonald, unpublished *F date verified: 28.09.1999 *F *F locus name: DMTRXIII3 *F accession number: Z50152 *F cytogenetic position: 88B3 *F genetic position: 3-54.2 *F microsatellite repeat *F [position]: (AC)9 [26425] *F forward primer sequence *F [position]: gaccgtttgtttgccttgat [26278] *F reverse primer sequence *F [position]: tgcctgtacaagtctgaccg [26496] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.32 *F fragment length (bp): 219 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DROTROPI2 *F accession number: K03277 *F cytogenetic position: 88F5 *F genetic position: 3-54.2 *F microsatellite repeat *F [position]: (TA)11 [1947] *F forward primer sequence *F [position]: gtacatcccgaatcccacac [1920] *F reverse primer sequence *F [position]: aatacactgaaactgttggggg [2016] *F amplification *F properties: sim - null alleles present *F maps to clone: - *F *F heterozygosity: mel = 0.73; sim = 0.48; sec = *F 0.00 *F fragment length (bp): 97 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: AC006414* *F accession number: AC006414 *F cytogenetic position: 89A1-A5 *F genetic position: 3-58.2 *F microsatellite repeat *F [position]: (AC)10 [74940] *F forward primer sequence *F [position]: gaaagagctccaaggcaatcagg [75068] *F reverse primer sequence *F [position]: tgtttcccaggacaggataagcg [74874] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.72; sec = 0.00 *F fragment length (bp): 195 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMU31961 *F accession number: U31961 *F cytogenetic position: 89E *F genetic position: 3-59.5 *F microsatellite repeat *F [position]: (AC)19 [49029] *F forward primer sequence *F [position]: atctgagtgcttgtgcaa [48965] *F reverse primer sequence *F [position]: cctcgacgaagttgcagg [49309] *F amplification *F properties: fine *F maps to clone: DS07696 *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 345 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROABDB *F accession number: L07835 *F cytogenetic position: 89E1-E2 *F genetic position: 3-59 *F microsatellite repeat *F [position]: (AC)19 [49029] *F forward primer sequence *F [position]: cttccaagtcaccaggac [49003] *F reverse primer sequence *F [position]: cacaccgacaacacaagacc [49150] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 148 *F literature reference: Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DROABDB *F accession number: L07835 *F cytogenetic position: 89E1-E2 *F genetic position: 3-59 *F microsatellite repeat *F [position]: (AC)19 [49029] *F forward primer sequence *F [position]: cagtgtacgtgtgcataacgc [48887] *F reverse primer sequence *F [position]: cgacaacatatccacatcgc [49178] *F amplification *F properties: sim/sec - not tested *F maps to clone: DS07696 *F heterozygosity: - *F fragment length (bp): 292 *F literature reference: J. Gockel, unpublished *F date verified: 28.09.1999 *F *F locus name: DMEHAB *F accession number: X72303 *F cytogenetic position: 90B1-B2 *F genetic position: 3-59 *F microsatellite repeat *F [position]: (AGCC)5 [218] *F forward primer sequence *F [position]: atttattagttttttgctaatacttgc [7] *F reverse primer sequence *F [position]: agagttcttgttgtatttatac [368] *F amplification *F properties: sim nd *F maps to clone: - *F heterozygosity: sim = 0.64; sec = 0.32 *F fragment length (bp): 362 *F literature reference: Schug et al. 1997 *F date verified: 28.09.1999 *F *F locus name: DRONANOS *F accession number: M72421 *F cytogenetic position: 91F13 *F genetic position: 3-66.2 *F microsatellite repeat *F [position]: (TA)18 [3143] *F forward primer sequence *F [position]: cgcaagtattcatttcaacaca [3115] *F reverse primer sequence *F [position]: tgctggcggttgtttcat [3244] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.77; sim = 0.72; sec = *F 0.00 *F fragment length (bp): 130 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DROHOXNK4 *F accession number: M27292 *F cytogenetic position: 93E1 *F genetic position: 3-72 *F microsatellite repeat *F [position]: (AGC)5 [510] *F forward primer sequence *F [position]: ctgaagttgaagtccgagcc [416] *F reverse primer sequence *F [position]: tacatgtgctgcatctgttgc [558] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.32; sec = 0.00 *F fragment length (bp): 143 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DMU25686 *F accession number: U25686 *F cytogenetic position: 93F *F genetic position: 3-73 *F microsatellite repeat *F [position]: (AT)15 [32] *F forward primer sequence *F [position]: cgataatttactctgtgctcc [9] *F reverse primer sequence *F [position]: cagctcacacaaaaggcaaa [161] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.76; sim = 0.00; sec = *F 0.00 *F fragment length (bp): 153 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMPOINT1A *F accession number: X69166 *F cytogenetic position: 94F1-F2 *F genetic position: 3-79 *F microsatellite repeat *F [position]: (ATC)7 [1270] *F forward primer sequence *F [position]: caataacaattgccacacgg [1187] *F reverse primer sequence *F [position]: aattggtgatgcggatgg [1315] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.00; sec = 0.00 *F fragment length (bp): 129 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DMU1951 *F accession number: X53543 *F cytogenetic position: 95C *F genetic position: 3-81 *F microsatellite repeat *F [position]: (TA)16 [1948] *F forward primer sequence *F [position]: gggtctttctgcttcagttacc [1890] *F reverse primer sequence *F [position]: ggaatacacgaatccccctt [2087] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.62; sim = 0.00; sec = *F 0.00 *F fragment length (bp): 198 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DMTF125 *F accession number: X98235 *F cytogenetic position: 95C6-C8 *F genetic position: 3-81.5 *F microsatellite repeat *F [position]: (CAG)6 [4889] *F forward primer sequence *F [position]: ctcgagcgggccatacaaga [4742] *F reverse primer sequence *F [position]: tgattgaagaggccactcaa [5046] *F amplification *F properties: fine *F maps to clone: - *F *F heterozygosity: mel = 0.84; sim = 0.72; sec = *F 0.00 *F fragment length (bp): 305 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 *F *F locus name: DMMSIDNA *F accession number: X79340 *F cytogenetic position: 96E1-E4 *F genetic position: 3-87 *F microsatellite repeat *F [position]: (GC)6 [3335] *F forward primer sequence *F [position]: tgcacatactcctttcaattcg [3273] *F reverse primer sequence *F [position]: aacaggactcggaacaatgc [3398] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.32; sec = 0.00 *F fragment length (bp): 126 *F literature reference: Schug et al. 1998 *F date verified: 28.09.1999 *F *F locus name: DROROUGH *F accession number: M23629 *F cytogenetic position: 97D5 *F genetic position: 3-91.1 *F microsatellite repeat *F [position]: (GCT)11 [5609] *F forward primer sequence *F [position]: aagcaatgccacacaatgag [5590] *F reverse primer sequence *F [position]: cggttattttttttccttggc [5685] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 96 *F literature reference: I. Colson, unpublished *F date verified: 28.09.1999 *F *F locus name: DROLMALK *F accession number: L37323 *F cytogenetic position: 98B *F genetic position: 3-98 *F microsatellite repeat *F [position]: (AAC)6 [855] *F forward primer sequence *F [position]: gggtaatcccttgctaatatgg [732] *F reverse primer sequence *F [position]: aatggttgtcgctaaaagtt [929] *F amplification *F properties: sim/sec - no amplification *F maps to clone: - *F heterozygosity: - *F fragment length (bp): 198 *F literature reference: Goldstein & Clark 1995 *F date verified: 28.09.1999 *F *F locus name: DMU43090 *F accession number: U43090 *F cytogenetic position: 99D6-D9 *F genetic position: 3-99 *F microsatellite repeat *F [position]: (CAG)8 [2911] *F forward primer sequence *F [position]: tgcacccagcaataccagta [2787] *F reverse primer sequence *F [position]: gctgttgtcgtggtgctg [2976] *F amplification *F properties: fine *F maps to clone: - *F heterozygosity: sim = 0.56; sec = 0.00 *F fragment length (bp): 190 *F literature reference: Colson et al. 1999 *F date verified: 28.09.1999 # *U FBrf0112122 *a Vienna lab *b ?. *t 1999.7.24 *T personal communication to FlyBase *u *F http://i122server.vu-wien.ac.at/Microsatellite%20Loci/Loci%20Titelpage.html *F July 24 1999 *F Last Updated on 14.07.1999 *F Converted from Excel files by Aubrey de Grey. *F *F *F D. melanogaster *F D.simulans *F D.sechellia *F D. mauritiana *F Locus Repeat cyt. location No. alleles Het. Variance *F Size range No. alleles Het. Variance Size range *F No. alleles Het. Variance Size range No. alleles *F Het. Variance Size range Annealing temp. Primer *F forward reverse internal reference *F DMC30B8 (AG)17 2F1-2F6 14 0.79 9.96 130-162 2 0.49 *F 0.99 103-105 1 \* \* 107 1 \* \* *F 105 53 atagcatttgccagtgccagta ttgctcgctcgctcgctcactc 799 / *F 800 *F DS06335a (GT)15 3C1-C6 8 0.76 5.57 87-107 3 *F 0.34 0.75 91-99 1 0.00 0.00 97 7 0.89 *F 5.09 93-105 53 actgtaattgctgttctatgt cgcacactgggacacaaaa *F 108 / 62 *F DS06335b (CA)12 3C1-C6 4 0.34 1.78 136-145 2 *F 0.06 0.03 126-128 1 0.00 0.00 126 4 0.27 *F 2.03 114-130 50 gcacaatcacatcgtattcact attgttgttgctgcgattt *F DS00036 (CA)10 4A3-A5 4 0.55 1.03 95-101 n.a. n.a. *F n.a. n.a. n.a. n.a. n.a. n.a. *F n.a. 48 ccccgcacacacacacacata tgcttattgtttaatttacct *F 639 / 640 *F DS00146 (GT)10 4A4-B2 2 0.26 0.13 117-119 3 0.34 *F 0.73 103-111 1 0.00 0.00 109 3 0.39 3.69 *F 99-113 53 gagtcaacgagccagcaaagt aacaatacagagcagcacacg 111 / *F 112 *F DS00589 (CA)11 5C1-C2 0.56 8.50 95-95 n.a. n.a. *F n.a. 78 n.a. n.a. n.a. 80 n.a. n.a. n.a. *F 78 52 cgttttttatttgcgggcag acatccctctctttcgcttc 163 / *F 164 *F DS06329 (GT)12 6C1-C2 4 0.68 7.63 78-92 n.a. n.a. *F n.a. n.a. n.a. n.a. n.a. n.a. *F n.a. 54 cctggttgctcccgctgc ttccgagatcacctgaga *F 637 / 638 *F DS04440 (GT)9 6E1-E2 3 0.54 6.91 112-128 2 0.10 *F 0.05 107-109 n.a. n.a. n.a. n.a. n.a. *F n.a. 55 ttctcccaccgtaacgccctat acacaacatccgttgctgctgt *F 227 / 228 *F sex lethal (AT)9 6F4-7B3 n.d. *F n.d. n.d. n.d. n.d. n.d. n.d. n.d. *F n.d. 44.5 ttttgtcgttttcgttatg tttgtatgttcctcacttta *F 3 / 2 *F DS09021 (GT)12 8B5-B8 8 0.81 36.19 148-180 3 0.57 *F 0.37 140-144 1 0.00 0.00 140 1 0.00 0.00 *F 146 52 ttcccgcatatgtgtgag tttcgtgtacttctcggtgc 105 / *F 106 *F DS01391 (GT)10 9A1-A2 4 0.32 4.31 127-141 1 0.00 *F 0.00 125-125 1 0.00 0.00 136 1 0.00 0.00 *F 125 57 gcctgctgcagtcgcatgtg ccagcggcatacgtgtaaac 633 / *F 634 *F DS00907 (AG)13 10A1-A2 3 0.60 1.15 232-238 n.a. n.a. *F n.a. n.a. n.a. n.a. n.a. n.a. *F n.a. 56 agatagagcggctggcaaca agcagagcagagccagcactt *F 238 / 292 *F DS01551 (CA)10 10D5-E4 2 0.26 0.13 197-199 3 0.18 *F 0.27 193-197 2 0.07 0.04 193-195 1 0.00 0.00 *F 193 51 caacaacaaaaacagcaacga tgccaactgcaaaaacacaga 145 / *F 146 *F Dmtena (TA)4CC(AT)14 11A6 6 0.70 1.98 91-101 2 *F 0.06 0.50 79-87 2 0.14 0.07 89-91 1 0.00 *F 0.00 87 55 ctcttagtgcgcagggattc gagtcgctcaatggcagg *F 66 / 67 *F DS00314 (TG)8 12D1-D2 1 0.00 0.00 163-221 1 0.00 *F 0.00 148-158 1 0.00 0.00 156 1 0.00 0.00 *F 150-158 48 gtgactgtgttgattccgtg ttggaaaagcgaaaagtaag 165 / *F 166 *F DS09020 (GT)8(GC)4 15A1-A4 4 0.47 0.75 138-144 4 *F 0.44 0.63 125-131 1 0.00 0.00 127 3 0.43 *F 2.32 125-134 51 ctaaacaggatgcaggacaac gcgatcaaggttaaatggttc *F 147 / 148 *F DS00265a (GA)11 16A1-A6 n.a. *F n.a. n.a. n.a. n.a. n.a. n.a. n.a. *F n.a. 58 agtgggtttcctcggcgtgc tttgcccactcagcttgcttcg *F 229 / 230 *F *F *F D. melanogaster *F D.simulans *F D.sechellia *F D. mauritiana *F Locus Repeat cyt. location No. alleles Het. Variance *F Size range No. alleles Het. Variance Size range *F No. alleles Het. Variance Size range No. alleles *F Het. Variance Size range Annealing temp. Primer *F forward reverse internal reference *F Cd36 (GA)17 21B7-21C3 1 0.00 0.00 156 2 *F 0.23 0.11 158-160 1 0.00 0.00 162 1 0.00 *F 0.00 158 49.5 tgtctaatcttacctaaatac atttatgctttaagctaagtc *F 346/347 *F Eno2 (TA)20 22A1-B2 17 0.86 26.19 *F 111-147 *F 45.6 gctggtgcgtttccaagaag aagatgctgtgagaaataag 720/721 *F Eno-TA (TA)9 22A2-A5 12 0.73 5.04 *F 146-169 *F 51.3 tgcattctggcgacgctgac aaatctcccacaaatgtcac 687/688 *F Eno-TC (TC)9 22A2-A5 6 0.20 3.30 *F 123-136 *F 46.8 tcataattccatgttccacac taaatagtcacttagcttgaaag 665/666 *F Eno-CA (CA)9 22A2-A5 6 0.62 12.74 *F 168-180 *F 51.3 taaaattgcacaacataacttg tccgtgtgcgtatgttcctgtg 618/619 *F Z50409 (GT)7 22B1-B9 3 0.67 7.39 126-138 3 0.39 *F 0.94 125-133 1 0.00 0.00 129 4 0.59 0.43 *F 125-129 50 gcaaaacggaaaagcaaaaga gtgtgttccacttccttctac 093/094 *F AC005749 (GT)4GG(GT)17 22B3-22C1 16 0.83 20.42 *F 129-171 3 0.65 2.39 138-142 1 \* \* 140 *F 2 \* \* 138-142 50 tacaaaaccgacgcataaaatac *F tagatacctagttcggctaag 924/925 *F Droyanetsb (TG)21 22C 15 0.74 16.69 84-122 3 *F 0.51 0.29 80-84 2 0.26 0.13 86-88 2 0.17 *F 0.09 82-84 55 taatggggaatgggtgaatg gccgtgctcttttctcttacg *F 151/152 *F Pkg-TA (TA)8 23A1-A3 10 0.56 3.38 *F 111-122 *F 48.3 aatcaaagcaacaacaacagtg tgcgtagccgtgccatactc 716/717 *F Pkg-TC (TC)9 23A1-A3 7 0.73 7.39 *F 104-118 *F 50.5 gaaaagcggaaaagccacac ttattttgttgccttctctg 446/447 *F Pkg-GT (GT)9 23A1-A3 11 0.62 45.50 *F 139-177 *F 53 cctcgtattcaccccatctc tggagacgacgacgaacttg 448/449 *F DS01340 (AG)9T(GA)3 24A1-A2 7 0.65 1.65 176-190 3 *F 0.69 0.62 159-163 1 0.00 0.00 165 5 0.74 *F 1.78 172-182 55 ggagcgcaatgctgtttaagt ggagtagtgcctgtctcggac *F 149/150 *F Dm0600-TA (TA)11 24C3-D1 10 0.61 3.98 *F 153-175 *F 50.6 ggaagaaagagagggagaatc tgtgggaaatgaaaacgaaag 681/682 *F Dm0600-TC (TC)9 24C3-D1 10 0.43 12.31 *F 112-132 *F 50 accgtcaacaaaaagcatac gaaagtcaaaagccgcacac 444/445 *F Dm0600-CA (CA)9 24C3-D1 9 0.63 10.35 *F 146-170 *F 50.8 tgcctccgaacctctgagac cctgcctctgtatctgtatc 442/443 *F ft-TA (TA)7 24E1-E4 5 0.10 25.68 *F 132-196 *F 53 acaggctaagcggcaacaac gcgggtgctacgtgtgactc 689/690 *F ft-TC (TC)9 24E1-E4 16 0.70 25.75 *F 154-178 *F 51.9 ctttgtcccctcattttctc aatggttggatgtggatgtg 434/435 *F ft-CA (CA)9 24E1-E4 5 0.46 18.90 *F 130-144 *F 54.9 gtgggttgaaggggaagaag cgcagtggaacagccagttg 436/437 *F AC005270 (AC)19 24E1-F1 13 0.82 11.22 125-149 6 *F 0.09 2.01 119-130 1 \* \* 125 2 \* *F \* 123-125 57.6 cggcagacgacacttgacac gagaccagccgccttgacta *F 858/859 *F Drogpdha (CT)7 25F5-26 A 3 0.47 0.08 132-134 *F 3 0.52 0.19 129-132 1 0.00 0.00 130 1 *F 0.00 0.00 130 53 cattggaaaagtgagcggat *F cggacaacaacaaatcgttg 153/154 *F DS00168 (GT)9 26A1-A2 1 0.00 0.00 112 3 0.57 *F 0.39 117-121 1 0.00 0.00 117 n.a. n.a. *F n.a. 57 ccgcttcctgtccgcttgc ggcgggaagagcatttgtt *F 643/644 *F Acp26Ab (CA)13 26B3-B5 5 0.65 1.30 160-168 4 0.62 *F 1.84 141-150 1 0.00 0.00 148 1 0.00 0.00 *F 142 50 cacaaaggactcggcaagcac atcctccaaatgaaattacag 350/351 *F Dm2337.2 (TA)23 26F1-27C2 14 0.76 22.96 *F 162-198 *F 48.3 cgtagctgaattttcatgtc cgaaatcagaggtcacttac 722/723 *F Dm2337.2 (TA)23 26F1-27C3 *F 3 0.25 0.74 128-134 4 \* \* 152-162 3 *F \* \* 130-136 52.8 tcgaacgagaccgtagctgaat *F gccgcactcaaaactgccactg 852/853 *F Dm2337 (CA)15 27A1-B1 6 0.79 2.34 150-160 4 0.55 *F 0.77 139-148 1 0.00 0.00 134 3 0.50 0.31 *F 144-148 57.7 ccatttttccgccccaatgtc acggtgggaaagcgtggaaag 341/343 *F Droninac (AT)10 28A1-A3 3 0.45 0.26 136-140 3 *F 0.42 1.22 133-140 n.a. n.a. n.a. 133 n.a. n.a. *F n.a. 130-140 56 tttgtcaatctctcacagcagg gcccgagtacatttattcaagc *F 155/156 *F su.var (TG)12 29A5-B4 6 0.68 4.18 164-176 6 0.65 *F 13.60 133-189 1 0.00 0.00 172 2 0.52 0.26 *F 162-164 56.2 ggttgctgggagaaagac gccacacattcgcatctc 414/415 *F DS08088 (TG)12 29C1-C4 7 0.43 1.54 127-141 4 0.37 *F 0.99 134-142 1 0.00 0.00 136 n.a. n.a. *F n.a. 54.3 gaggcgacaggaagttttac ttgtgtttcgttccggtttc *F 629/630 *F DS01054 (GT)7 30A1-A2 1 0.00 0.00 180 5 0.51 *F 1.42 164-174 2 0.07 0.01 163-164 n.a. n.a. n.a. *F 152-174 57 tcctgctcctcgggctttac aaaacagccacttcacacac 256/257 *F DS03018 (AC)8 30A3-A6 4 0.52 0.93 95-101 3 0.54 *F 0.41 97-101 1 0.00 0.00 97 4 0.52 1.27 *F 91-101 51.4 cgttcatgaccttgaaaagc gtgtgaaatgtaggggaaac 631/632 *F DS00058/2 (TG)13 31A1-A2 4 0.55 3.95 123-131 1 *F 0.00 0.00 115 1 0.00 0.00 115 n.a. n.a. *F n.a. 57 tatgggtgggtagctgtgac caacgggaacgccatctaac *F 623/624 *F DS04172 (CA)8G(CA)6 32A3-D4 13 0.91 10.03 75-101 3 *F 0.28 0.04 74-76 1 0.00 0.00 75 2 0.30 *F 0.04 74-75 55.3 gaacggaaatcattcttctg cactacccagcactgcaagg *F 627/628 *F G410 (TC)11(TG)4 33E9-E10 12 0.74 14.07 124-162 *F 5 0.56 0.68 122-128 2 0.09 0.39 126-132 3 *F 0.45 1.04 124-132 53 ttcggctctttgtttgcttg *F aagcttaaaccgatcgaaaac 43/45 *F L49403 (TA)19 35B2-B10 19 0.85 58.38 *F 139-235 *F 41.5 aacttgaggggttgattc gaagcgaagggggttttattc 854/855 *F AC004118 (TC)18 35B2-B3 16 0.78 7.28 109-141 1 *F 0.00 0.00 96 1 \* \* 96 1 \* *F \* 96 51.2 gcacacggcgttcgcactct cgagttcacttgacttgtct *F 842/843 *F Adh-TA (TA)9 35B2-B4 6 0.58 1.99 *F 163-173 *F 51.7 gcaactcaataaacccaatttc caaaaagccacaaatcgcagtc 675/676 *F Adh-TC (TC)11 35B2-B4 9 0.69 22.63 *F 132-158 *F 54.6 cagcaccagcatccaagtac agtctctgtggcagtgtgag 430/431 *F Adh-TG (TG)11 35B2-B4 4 0.15 0.35 *F 144-152 *F 51.2 ttgaaagtgttgtcgctctg acaaagaggaagagggcatc 432/433 *F 6744 (CT)8 35E6-F5 3 0.26 0.14 90-94 3 0.40 *F 0.23 90-94 2 0.14 0.07 92-94 2 0.25 0.12 *F 92-94 56 cgctaagagtcgctctccat aaattgtttgcccgtctcac 322/323 *F cact-TA (TA)9 35F1-F6 10 0.59 8.85 *F 146-178 *F 48.2 gcacgaaaacggaataacatag ggcaaaagaaaacaacggactg 677/678 *F cact-TC (TC)9 35F1-F6 5 0.15 0.47 *F 110-118 *F 54.2 aaccagacgtagcgaaaaac aagagagggagcacaacatc 426/427 *F cact-TG (TG)9 35F1-F6 5 0.54 1.28 *F 148-155 *F 53.1 ctatgaaacgatgggcaaag tgacattaaaagggcaaaac 428/429 *F L49408 (TA)25 35F5-F12 24 0.89 15.99 101-157 7 *F 0.83 28.25 100-116 1 \* \* 104 2 \* *F \* 102-104 47.3 tcagagacatatccaaacacc acacacatttccattcctcag *F 922/923 *F DS00762 (GT)6T(TG)7 37B1-B2 3 0.53 1.91 114-129 4 *F 0.52 0.66 114-120 1 0.00 0.00 117 7 0.83 *F 10.97 112-134 52 gacagttgcaggcgcaccaac gtagcgtgtgcatttgacact *F 320/321 *F DS09065/1 (GT)14 38A1-A2 0.70 5.57 n.a. *F n.a. n.a. n.a. n.a. n.a. n.a. n.a. *F n.a. 55.3 ggtttcattccagcagaggg ccttcatttggcagcgtca *F 199/200 *F Dm0332-TA (TA)9 38B5-C2 7 0.65 1.05 *F 130-137 *F 49 caacttcccgaacaccaattac cagcagttcattaaaccgacac 697/698 *F Dm0332-TC (TC)8 38B5-C2 4 0.36 0.94 *F 107-113 *F 54.6 tatcctgccaccactgaatc aactgtttgcgttgcgtgtg 656/657 *F Dm0332-CA (CA)9 38B5-C2 7 0.38 32.60 *F 118-146 *F 54.3 ggctctgtggaaaactcaac gtgtggacgtgcggcttttg 649/667 *F Cad-TA (TA)9 38D4-E1 8 0.72 11.68 *F 153-169 *F 49.8 caactcatccccgaaataag acgaaaccgtgaaaagattg 679/680 *F Cad-GA (GA)11 38D4-E1 7 0.47 4.64 *F 138-152 *F 55.7 aggcactctctgggcgaaac cgtcactaggtcggggtatc 450/451 *F Cad-CA (CA)11 38D4-E1 10 0.75 9.74 *F 130-154 *F 52.8 gaaccccaagccgaggattg cacagccagaccagatgtag 452/453 *F DS07289 (TG)8 41E3-E6 2 0.48 0.24 130-132 1 0.00 *F 0.00 122 1 0.00 0.00 122 1 0.00 0.00 *F 122 54 ctcgttctgacgtccgtatc cttctctaatggcaaacacg 550/551 *F tor-TA (TA)9 43B3-C5 6 0.27 1.61 *F 149-161 *F 48.6 atatgttcgcctggctacac atttctcttgtttggctatg 699/700 *F tor-TC (TC)8 43B3-C5 2 0.37 0.75 *F 109-111 *F 56.2 aacgggagcaagagtgtaag ctttttcgccgccccagcag 660/661 *F tor-GT (GT)11 43B3-C5 7 0.53 8.21 *F 146-160 *F 51.5 ccttgacatggcacagagtc tgatggaatgacaggctaag 658/659 *F tor (CA)13 43B3-C5 5 0.74 1.49 102-112 4 0.39 *F 0.47 102-110 2 0.07 0.04 108-110 2 0.17 0.09 *F 104-106 54 tgcagtcatcaatggctaatc tgatttcccccgtccgaagtg 418/419 *F 5915 (CT)21 44D5-E4 6 0.46 6.08 114-130 4 0.75 *F 1.32 104-110 1 0.00 0.00 104 4 0.58 3.09 *F 104-118 54 atgcccctgactctctccac gagccggttgcatgtaag 316/317 *F Drogpad (GT)9 47A 5 0.52 1.99 158-191 6 0.52 *F 1.14 157-165 1 0.00 0.00 148 2 0.17 0.35 *F 152-156 56 gaaataggaatcattttgaatggc *F aattaaaaacaaaaaacctgagcg 157/158 *F Dmmp20 (CA)10 49F9-F13 4 0.57 0.22 86-93 4 *F 0.62 7.88 71-88 2 0.14 2.48 76-88 3 0.71 *F 1.83 76-84 55 catgcaaatgagcagtactttg tattttcacacatttccaatcg *F 159/160 *F Dm0620 (GT)9 51E5-E8 4 0.53 0.79 133-143 4 0.73 *F 1.32 139-145 1 0.00 0.00 139 3 0.57 0.66 *F 139-143 55 gagacaccccttgacgagtg ctcaaaacaaacccagtctc 406/407 *F DS00541 (CA)10 52C1-C2 4 0.32 0.60 138-148 8 0.76 *F 21.10 132-158 2 0.21 1.64 130-138 4 0.49 0.41 *F 142-148 57 gcacgaggtatcgcacaaag tccgtcgttcgctgctgggt 625/626 *F Sca1.b (GT)18 52D1-D15 9 0.73 2.65 147-167 3 *F 0.26 1.25 142-146 1 \* \* 146 2 \* *F \* 142-146 54.2 aattcattccgtttttcaagtg accaaggtgtgggtgaagttgt *F 773/774 *F AC00556 (TA)20 52D1-D15 17 0.81 6.47 *F 150-188 *F 46.4 gagtaaaaagacgctcagt tctactaccacccgataac 846/847 *F AC004516 (CA)18 52D1-D15 17 0.78 10.42 90-126 *F 4 0.53 3.03 104-114 1 \* \* 112 1 *F \* \* 112 53 cttgctccacgcacttacac *F gcttgctgattttcgcatta 862/863 *F AC004248 (CA)22 52D2-D15 12 0.37 38.04 *F 98-140 *F 58 aatttccctcgcactgacac tgcagcccgcacttccttac 866/867 *F sli (CA)21 52D9-D15 2 0.13 0.06 134-136 4 *F 0.48 4.75 134-144 4 0.67 4.37 150-160 5 0.78 *F 2.68 144-154 57.5 caatttccctcgcactgacac cggaaacgaacgggcgataag *F 422/423 *F Pkc53E-TA (TA)8 53D1-D5 5 0.10 2.19 *F 124-137 *F 51 gtaacaacccatcccaagtg tcctcatggctcccgattac 693/694 *F Pkc53E-GA (GA)11 53D1-D5 12 0.50 8.08 *F 117-149 *F 50.9 cagagaacagagcgagtgac ccgtaagcaccttccatgtc 440/441 *F Pkc53E-CA (CA)11 53D1-D5 15 0.74 251.79 *F 120-144 *F 51.7 taatcaagtgcaatccaacaa tgcaaaacggaaccaggcagac 614/615 *F AC004641 (CA)22 53D1-E2 25 0.91 26.01 92-154 3 *F 0.44 11.93 96-108 1 \* \* 106 3 \* *F \* 98-108 51.4 atcacaactggaccctctat aatttcacaaccaacaacta *F 860/861 *F DS00361 (CA)8 54B1-B2 6 0.37 6.63 132-155 7 0.81 *F 6.96 143-159 1 0.00 0.00 151 6 0.79 1.81 *F 143-154 52 caaccacccacaagcacac cctctccggttgggctac no CS *F 917/918 *F DS00361c (TG)7 54B1-B2 4 0.33 0.55 120-128 3 *F 0.49 0.31 122-126 1 0.00 0.00 124 4 0.64 *F 0.66 122-127 51 cgttaagcgtcgaaaatag ctttagcactttgcattcc *F 554/555 *F DS00144 (CT)8 55A1-B1 3 0.10 0.02 115-117 3 0.66 *F 0.56 115-119 3 0.33 0.79 113-121 3 0.54 0.32 *F 115-119 52 gctcagacccaaatggcgtag aaactcaaactctccagcgag 91/92 *F Ote-TA (TA)8 55A2-B1 4 0.23 0.79 *F 141-147 *F 48.3 aaaggactacagcagcactc agacagcggctcagggtaag 691/692 *F Ote-GA (GA)11 55A2-B1 7 0.53 4.66 *F 106-118 *F 55.4 gttgattgcttgacaaattgcc ctgccccctgtcgccatttctc 620/621 *F Ote-CA (CA)12 55A2-B1 13 0.74 24.17 *F 162-198 *F 49.7 gcagtcgcaacgtagggagaag agtatgctacacgaatttgaag 616/617 *F Dpt-TA (TA)8 55F1-F6 15 0.80 12.47 *F 150-171 *F 49.7 agaaaagcggcaaccaagtc tctcacttccccctctttac 681/682 *F Dpt-TC (TC)9 55F1-F6 4 0.04 0.16 *F 174-180 *F 53.9 gcaactcttgcgtttttatc gaccacctcgctccctcttg 456/457 *F Dpt-GT (GT)9 55F1-F6 8 0.56 4.98 *F 126-139 *F 50.6 gcaaaccgttttccatttac cataactaactggggacttg 612/613 *F DS08687a (TG)11 57C5-D1 5 0.38 0.52 180-186 7 *F 0.72 7.19 168-186 3 0.41 3.54 176-188 4 0.71 *F 2.68 176-186 54 tgatttggactgagatcagg gcccaacgaatcatttcac *F 313/197 *F DS08687b (GT)9GC(GT)3 57C5-D1 10 0.79 2.75 156-173 *F 3 0.42 1.76 134-145 3 0.17 0.21 135-139 6 *F 0.83 10.94 114-143 52 tgtcgagagcagcagcagt *F ttgccttccctgttacag no CS 891/892 *F AC002446 (TC)18 58B1-B2 14 0.71 9.40 144-188 5 *F 0.74 5.76 136-144 3 \* \* 146-150 2 \* *F \* 138-142 51.2 tccttattcggtctacaaatct atacacatgcacatccgtatag *F 840/841 *F Dm1639-TA (TA)9 58B10-C4 8 0.65 4.98 *F 153-175 *F 50.3 aaacaaaagcctgtcaagtgtg tgtagtcaaggaggtttgagtg 730/731 *F Dm1639-TC (TC)12 58B10-C4 8 0.66 5.88 *F 102-120 *F 55.2 gcctcgctccgtcccgttcc cgattgtttccattgttcac 535/536 *F Dm1639-CA (CA)11 58B10-C4 13 0.58 39.01 *F 139-177 *F 52.9 cattggtttcttccgttttc cgtgccaggttgccttttag 533/534 *F Z32225 (GT)10 58C1-C7 4 0.16 5.67 n.d. n.d. *F n.d. n.d. n.d. n.d. n.d. n.d. *F n.d. 52 atggcaaccactgctgacac gcatcctggaagtcctttag *F 99/100 *F Z31849 (GT)10 58C1-C7 3 0.48 2.32 137-147 n.a. n.a. *F n.a. 1 0.00 0.00 134 2 0.19 0.09 *F 131-133 53 cccattaaggccgataagtc gagatagctctgtttgccag 101/102 *F G411 (CT)6(TG)4 58C2-C5 3 0.40 0.23 158-162 6 *F 0.77 3.10 156-168 2 0.24 0.27 160-163 4 0.78 *F 0.56 160-164 50.5 attgctgtttggagtttgttg ggtcgcagggacacaattcac *F 46/47 *F DS08011 (GT)8 59A1-B2 7 0.78 6.59 102-122 5 0.62 *F 6.18 100-112 1 0.00 0.00 104 1 0.00 0.00 *F 102 53 agccacagccatgcgtttaac cacacgctgacaggatctact 70/71 *F Dromhc (CA)13 60 E9 5 0.73 0.60 101-106 6 0.72 *F 3.02 89-101 1 0.00 0.00 91 4 0.72 2.16 *F 91-99 57 aaacccacacaacaactgca gacattaccgatattggatgca 161/162 *F *F *F D. melanogaster *F D.simulans *F D.sechellia *F D. mauritiana *F Locus Repeat cyt. location No. alleles Het. Variance *F Size range No. alleles Het. Variance size range *F No. alleles Het. Variance Size range No. alleles *F Het. Variance Size range Annealing temp. Primer *F forward reverse internal reference *F Antp-TA (TA)8 84A2-B2 4 0.47 1.06 *F 160-167 *F '46,4' agccgcaaatacttccaaac caagatttcgtcgttcatac 701/702 *F Antp-TC (TC)9 84A2-B2 5 0.25 1.62 *F 146-163 *F '50,0' gctttcgatcccatagatac cgggctcatctaatgatatg 663/664 *F Antp-GT (GT)8 84A2-B2 3 0.03 0.07 *F 135-139 *F '57,3' cccgccccttccctgctaac atatgtgtctgcgtcgtgtg 662/668 *F Antp 1 (GT)26 84B2-C2 29 0.93 51.07 *F 122-208 *F 50 cgttcgttcatgggcttttc ctctttaatatcggggttgg 728/729 *F Antp 1 (GT)26 84B2-C3 7 0.69 *F 14.21 101-113 1 \* \* 101 1 \* \* *F 101 55 taatatcggggttgggggtg gttcgttcatgggcttttct 864/865 *F DMTRXIII (CT)23 88B3 37 0.95 23.77 92-150 5 *F 0.65 5.99 100-110 1 \* \* 102 1 \* *F \* 102 55 aggtgtgtgcgtttgctctc gccgaacgaacacgaagata *F 859/857 *F *F *F D. melanogaster *F D.simulans *F D.sechellia *F D. mauritiana *F Locus Repeat cyt. location No. alleles Het. Variance *F Size range No. alleles Het. Variance Size range *F No. alleles Het. Variance Size range No. alleles *F Het. Variance Size range Annealing temp. Primer *F forward reverse internal reference *F M18 (GT)27 unmapped 33 0.95 53.48 152-234 5 *F 0.77 17.66 148-160 1 \* \* 156 3 \* *F \* 134-158 55.2 cccgtcaccaaagtgaaaac tcaggaatgttagcaagcga *F M12 (GT)25 unmapped 35 0.94 88.83 *F 166-288 *F 56 tccgtctcctttgtggctat cagtgcaaagaaaacggtga *F DM73.alt unmapped 32 0.95 52.56 *F 88-166 *F 54 accaaagtgaaaacacaacaag acgctgctcccctcacacacac *F DMC11F6 (TA)20 X-chr 1 0.00 *F 0.00 120 1 \* \* 122 2 \* \* *F 132-152 50 acttttagagggcggatg gactgaactgggggtatc *F *F *F *F *F sechellia: *F *F D. melanogaster *F D.simulans *F D.sechellia *F D. mauritiana *F Locus Repeat cyt. location No. alleles Het. Variance *F Size range No. alleles Het. Variance size range *F No. alleles Het. Variance Size range No. alleles *F Het. Variance Size range Annealing temp. Primer *F forward reverse internal reference *F sec 1 (AG)10 6 0.70 2.59 196-212 4 0.77 *F 1.28 200-206 2 0.07 0.04 202-204 2 0.45 0.23 *F 198-200 53 cggagagtggagattcgagag ggcccatgcgttcgtgtgtcg 294 / *F 295 *F sec 3 (GT)9 1 0.00 0.00 148 7 0.81 *F 17.89 146-174 1 0.00 0.00 151 6 0.85 5.49 *F 146-160 55 gtttccccaacgctgcgtgtg agtccaacttttgcctcagtc 298 / *F 299 *F sec 6 (AG)20 4 0.56 0.42 132-138 5 0.69 *F 4.51 122-143 8 0.83 9.86 138-166 2 0.09 0.05 *F 124-126 50 taaaataagggatcaaaagtc gcgcgtataatcacatatttc 300 / *F 301 *F sec 7 (GT)9 9 0.74 34.90 159-191 5 0.76 *F 4.26 145-155 1 0.00 0.00 149 4 0.64 2.76 *F 143-155 50 ggtatggtcgaaagaaatatc taactgtggctattttggtgac 302 / *F 303 *F sec 9 (GT)9 1 0.00 0.00 142 2 0.28 *F 0.04 138-139 2 0.07 0.14 137-141 2 0.26 0.03 *F 138-139 48 ttaataacattccaggagttg caaacaccaaaaaaccgtgta 356 / *F 357 *F sec 12 (GT)10 4 0.55 0.74 108-120 6 0.78 *F 2.79 106-120 4 0.27 1.38 106-115 3 0.69 0.76 *F 108-112 55.5 aaatcagcagggctgccaaac cgcacgcatacaaaagcatac 358 / *F 359 *F sec 13 (GT)7GG(GT)3 8 0.63 11.58 136-173 3 *F 0.23 0.32 136-142 2 0.52 0.26 134-136 4 0.40 *F 1.04 128-140 51 aatacatttaccatttgttag tacacagacacacaggattac *F 360 / 361 # *U FBrf0112123 *a Vienna lab *b ?. *t 1999.10.17 *T personal communication to FlyBase *u *F Last Updated on 21.09.1999 *F D. *F melanogaster *F D.simulans *F D.sechellia *F D. *F mauritiana *F Locus *F Repeat *F cyt. location *F No. *F alleles *F Het. *F Variance *F Size *F range *F No. *F alleles *F Het. *F Variance *F size *F range *F No. *F alleles *F Het. *F Variance *F Size *F range *F No. *F alleles *F Het. *F Variance *F Size *F range *F Annealing *F temp. *F Primer forward *F reverse *F internal *F reference *F Aprt.3 *F (GT)9 *F 61F3-62B11 *F 9 *F 0.74 *F 8.23 *F 133-151 *F 51.8 *F ctgggttgaatgtttgattggt *F accgaaaacaaaacgagaaaac *F 781/782 *F Mdr65.1 *F (TA)14 *F 64E11-65B5 *F 13 *F 0.86 *F 57.87 *F 88-120 *F 45.4 *F tgcgataacgtaatttactgac *F gctcgatcagatgaggattatc *F 783/784 *F Antp-TA *F (TA)8 *F 84A2-B2 *F 4 *F 0.47 *F 1.06 *F 160-167 *F 46.4 *F agccgcaaatacttccaaac *F caagatttcgtcgttcatac *F 701/702 *F Antp-TC *F (TC)9 *F 84A2-B2 *F 5 *F 0.25 *F 1.62 *F 146-163 *F 50.0 *F gctttcgatcccatagatac *F cgggctcatctaatgatatg *F 663/664 *F Antp-GT *F (GT)8 *F 84A2-B2 *F 3 *F 0.03 *F 0.07 *F 135-139 *F 57.3 *F cccgccccttccctgctaac *F atatgtgtctgcgtcgtgtg *F 662/668 *F Antp 1 *F (GT)26 *F 84B2-C2 *F 29 *F 0.93 *F 51.07 *F 122-208 *F 50.0 *F cgttcgttcatgggcttttc *F ctctttaatatcggggttgg *F 728/729 *F Antp 1 *F (GT)26 *F 84B2-C3 *F 7 *F 0.69 *F 14.21 *F 101-113 *F 1 *F \* *F \* *F 101 *F 1 *F \* *F \* *F 101 *F 54.6 *F taatatcggggttgggggtg *F gttcgttcatgggcttttct *F 864/865 *F DMTRXIII *F (CT)23 *F 88B3 *F 37 *F 0.95 *F 23.77 *F 92-150 *F 5 *F 0.65 *F 5.99 *F 100-110 *F 1 *F \* *F \* *F 102 *F 1 *F \* *F \* *F 102 *F 55.3 *F aggtgtgtgcgtttgctctc *F gccgaacgaacacgaagata *F 859/857 *F Last Updated on 21.09.1999 *F D. melanogaster microsatellites located on the X-chromosome *F D. *F melanogaster *F D.simulans *F D.sechellia *F D. *F mauritiana *F Locus *F Repeat *F cyt. *F location *F No. *F alleles *F Het. *F Variance *F Size *F range *F No. *F alleles *F Het. *F Variance *F Size *F range *F No. *F alleles *F Het. *F Variance *F Size *F range *F No. *F alleles *F Het. *F Variance *F Size *F range *F Annealing *F temp. *F Primer forward *F reverse *F internal *F reference *F DMC30B8 *F (AG)17 *F 2F1-2F6 *F 14 *F 0.79 *F 9.96 *F 130-162 *F 2 *F 0.49 *F 0.99 *F 103-105 *F 1 *F \* *F \* *F 107 *F 1 *F \* *F \* *F 105 *F 53 *F atagcatttgccagtgccagta *F ttgctcgctcgctcgctcactc *F 799 / 800 *F DS01001 *F (GT)9 *F 3A1-A4 *F 18 *F 0.71 *F 10.80 *F 208-241 *F 53 *F ccaacgcaacgcaaccag *F ctcccacccaaaatggaaata *F 258/259 *F DS06335a *F (GT)15 *F 3C1-C6 *F 8 *F 0.76 *F 5.57 *F 87-107 *F 3 *F 0.34 *F 0.75 *F 91-99 *F 1 *F 0.00 *F 0.00 *F 97 *F 7 *F 0.89 *F 5.09 *F 93-105 *F 53 *F actgtaattgctgttctatgt *F cgcacactgggacacaaaa *F 108 / 62 *F DS06335b *F (CA)12 *F 3C1-C6 *F 4 *F 0.34 *F 1.78 *F 136-145 *F 2 *F 0.06 *F 0.03 *F 126-128 *F 1 *F 0.00 *F 0.00 *F 126 *F 4 *F 0.27 *F 2.03 *F 114-130 *F 50 *F gcacaatcacatcgtattcact *F attgttgttgctgcgattt *F DS00036 *F (CA)10 *F 4A3-A5 *F 4 *F 0.55 *F 1.03 *F 95-101 *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F 48 *F ccccgcacacacacacacata *F tgcttattgtttaatttacct *F 639 / 640 *F DS00146 *F (GT)10 *F 4A4-B2 *F 2 *F 0.26 *F 0.13 *F 117-119 *F 3 *F 0.34 *F 0.73 *F 103-111 *F 1 *F 0.00 *F 0.00 *F 109 *F 3 *F 0.39 *F 3.69 *F 99-113 *F 53 *F gagtcaacgagccagcaaagt *F aacaatacagagcagcacacg *F 111 / 112 *F DS00589 *F (CA)11 *F 5C1-C2 *F 0.56 *F 8.50 *F 95-95 *F n.a. *F n.a. *F n.a. *F 78 *F n.a. *F n.a. *F n.a. *F 80 *F n.a. *F n.a. *F n.a. *F 78 *F 52 *F cgttttttatttgcgggcag *F acatccctctctttcgcttc *F 163 / 164 *F DS06329 *F (GT)12 *F 6C1-C2 *F 4 *F 0.68 *F 7.63 *F 78-92 *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F 54 *F cctggttgctcccgctgc *F ttccgagatcacctgaga *F 637 / 638 *F DS04440 *F (GT)9 *F 6E1-E2 *F 3 *F 0.54 *F 6.91 *F 112-128 *F 2 *F 0.10 *F 0.05 *F 107-109 *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F 55 *F ttctcccaccgtaacgccctat *F acacaacatccgttgctgctgt *F 227 / 228 *F sex lethal *F (AT)9 *F 6F4-7B3 *F n.d. *F n.d. *F n.d. *F n.d. *F n.d. *F n.d. *F n.d. *F n.d. *F n.d. *F 44.5 *F ttttgtcgttttcgttatg *F tttgtatgttcctcacttta *F 3 / 2 *F DS09021 *F (GT)12 *F 8B5-B8 *F 8 *F 0.81 *F 36.19 *F 148-180 *F 3 *F 0.57 *F 0.37 *F 140-144 *F 1 *F 0.00 *F 0.00 *F 140 *F 1 *F 0.00 *F 0.00 *F 146 *F 52 *F ttcccgcatatgtgtgag *F tttcgtgtacttctcggtgc *F 105 / 106 *F DS01391 *F (GT)10 *F 9A1-A2 *F 4 *F 0.32 *F 4.31 *F 127-141 *F 1 *F 0.00 *F 0.00 *F 125-125 *F 1 *F 0.00 *F 0.00 *F 136 *F 1 *F 0.00 *F 0.00 *F 125 *F 57 *F gcctgctgcagtcgcatgtg *F ccagcggcatacgtgtaaac *F 633 / 634 *F DS00907 *F (AG)13 *F 10A1-A2 *F 3 *F 0.60 *F 1.15 *F 232-238 *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F 56 *F agatagagcggctggcaaca *F agcagagcagagccagcactt *F 238 / 292 *F DS01551 *F (CA)10 *F 10D5-E4 *F 2 *F 0.26 *F 0.13 *F 197-199 *F 3 *F 0.18 *F 0.27 *F 193-197 *F 2 *F 0.07 *F 0.04 *F 193-195 *F 1 *F 0.00 *F 0.00 *F 193 *F 51 *F caacaacaaaaacagcaacga *F tgccaactgcaaaaacacaga *F 145 / 146 *F Dmtena *F (TA)4CC(AT)14 *F 11A6 *F 6 *F 0.70 *F 1.98 *F 91-101 *F 2 *F 0.06 *F 0.50 *F 79-87 *F 2 *F 0.14 *F 0.07 *F 89-91 *F 1 *F 0.00 *F 0.00 *F 87 *F 55 *F ctcttagtgcgcagggattc *F gagtcgctcaatggcagg *F 66 / 67 *F DS00314 *F (TG)8 *F 12D1-D2 *F 1 *F 0.00 *F 0.00 *F 163-221 *F 1 *F 0.00 *F 0.00 *F 148-158 *F 1 *F 0.00 *F 0.00 *F 156 *F 1 *F 0.00 *F 0.00 *F 150-158 *F 48 *F gtgactgtgttgattccgtg *F ttggaaaagcgaaaagtaag *F 165 / 166 *F 3451.2 *F (GGT)5 *F 14A3-A5 *F 9 *F 0.74 *F 19.27 *F 101-131 *F 55 *F gattttctcgttcagcacg *F cgctgttcaaagaagcact *F 21/22 *F DS09020 *F (GT)8(GC)4 *F 15A1-A4 *F 4 *F 0.47 *F 0.75 *F 138-144 *F 4 *F 0.44 *F 0.63 *F 125-131 *F 1 *F 0.00 *F 0.00 *F 127 *F 3 *F 0.43 *F 2.32 *F 125-134 *F 51 *F ctaaacaggatgcaggacaac *F gcgatcaaggttaaatggttc *F 147 / 148 *F DS00265a *F (GA)11 *F 16A1-A6 *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F 58 *F agtgggtttcctcggcgtgc *F tttgcccactcagcttgcttcg *F 229 / 230 *F DS00265b *F (AT)5 *F 16A1-A6 *F 7 *F 0.740 *F 9.480 *F 119-130 *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F n.a. *F 51 *F aacaatgctggcgacttcaag *F ctcctcgttctccgtcccttat *F 231/232 *F Last Updated on 21.09.1999 # *U FBrf0112136 *a Gao *b F.B. *t 1999.11.4 *T personal communication to FlyBase *u *F From fenbiao@itsa.ucsf.edu Wed Nov 03 22:25:06 1999 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Wed, 3 Nov 1999 22:25:06 +0000 *F Date: Wed, 3 Nov 1999 14:26:31 -0800 (PST) *F From: Fen-Biao Gao *F To: Chihiro Yamada *F Subject: flybase *F MIME-Version: 1.0 *F Dear Chihiro, *F .. *F By the way, there is a mistake in our Table 2. The cytological interval *F for tumbleweed is 49C-50D, and the interval for shrub is 44F-45E. The two *F was reversed in the Table. it would be nice if you could change that when *F you put the information into Flybase. *F Thanks. *F With best wishes. *F Fen-Biao *F Jan Lab *F \-- *F From cy200@gen.cam.ac.uk Wed Nov 03 15:05:12 1999 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Wed, 3 Nov 1999 15:05:12 +0000 *F To: ynjan@itsa.ucsf.edu *F Subject: FlyBase Query *F Cc: cy200@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Chihiro Yamada *F Date: Wed, 3 Nov 1999 15:07:20 +0000 *F Content-Length: 2721 *F Dear Dr Jan, *F I am currently curating your paper for FlyBase: *F Gao et al, 1999 Genes Dev. 13(19) 2549-2561. *F I have a few questions I was hoping you could answer for me. *F 1. yew allele *F In Table 2 you mention an allele of a your new gene yew. Do you have a *F name for this allele? (e.g. yew1) *F 2. short symbols, limber, ponderosa, cypress, saguaro, (yew) *F Also in table 2 you mention a number of new genes. Have you decided on short symbols for these genes? Here are a number of suggestions that haven't already been used, though if you want to use another symbol that hasn't been taken then that is, of course, no problem. I assume that 'ye *F limber *F (lim is already taken) *F lmb *F lmr *F lbr *F lmbr *F ponderosa *F (pon is already taken) *F pond *F pnd *F cypress *F (although cyp is not taken, Cyp1, Cyp4c3, and other similar *F symbols are already in use) *F cps *F cypr *F cyps *F saguaro *F (sag is already taken) *F sago *F sgu *F sgo *F sgr *F 3. UAS-GFP *F You use a UAS-GFP construct throughout your paper, there are a number *F in our records, could you tell me if the one you used in this paper is *F one of those below ( I have listed those that seem to be the most *F likely ones). If it is not, or you are not sure, could you tell me *F where you got the construct from, and any other papers you know that *F this construct has been used in before. *F Avic\GFPm6.Scer\UAS *F Brand, 1999, Methods Cell Biol. 58: 165--181 *F P{UAS-GFP.m6} *F UAS-mGFP6 *F J. Haseloff *F C.M. Davidson *F A.H. Brand *F Expression of GFP carrying amino acid substitutions (for *F brighter fluorescence, higher solubility and improved *F maturation and spectral properties) is governed by UAS *F regulatory sequences. *F Avic\GFPS65T.Scer\UAS *F Timmons et al., 1997, Dev. Genet. 20(4): 338--347 *F GFP is expressed under the control of UAS regulatory sequences *F Avic\GFPS65T.I167T.Scer\UAS *F Brand, 1999, Methods Cell Biol. 58: 165--181 *F P{UAS-GFP.S65T.I167T} *F UAS-GFP S65T/I167T *F C.M. Davidson *F A.H. Brand *F Expression of GFP carrying two amino acid substitutions for *F brighter fluorescence is governed by UAS regulatory sequences. *F Amino acid replacement: S65T. *F Amino acid replacement: I167T. *F Avic\GFPScer\UAS.cGa *F GFPUAS.cGa *F Santel et al., 1998, J. Cell Sci. 111(22): 3299--3309 *F Saccharomyces cerevisiae UAS construct a of Gonzalez-Gaitan *F P{UAS-GFP.G} *F M. Gonzalez-Gaitan *F UAS regulatory sequences drive expression of Avic\GFP *F Avic\GFPScer\UAS.cYa *F Yeh et al., 1995, Proc. Natl. Acad. Sci. USA 92(15): 7036--7040 *F Saccharomyces cerevisiae UAS construct a of Yeh *F P{UAS-GFP.Y} *F KpnI-EcoRI cDNA fragment of GFP is expressed under the *F transcriptional control of five GAL4 UAS sites. *F Thank you for answering these questions. *F Best Wishes, *F Chihiro # *U FBrf0112153 *a Ashburner *b M. *c J. *d Roote *e S.E. *f Lewis *g G.M. *h Rubin *t 1999.10.12 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Tue Oct 12 09:51:40 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 12 Oct 1999 09:51:40 +0100 *F To: gm119@gen.cam.ac.uk *F Subject: Adh *F Cc: ma11@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Tue, 12 Oct 1999 09:53:33 +0100 *F Content-Length: 190201 *F Gillian *F This is our working file. It can be taken as a personal communication to *F FlyBase from Ashburner, Misra, Roote, Lewis & Rubin. *F This version will have the new genes analysed since the paper was *F submitted. These should be curated. This file is authorative over the *F paper [except for the 'product' lines which were never u/dated]. *F Also ignore the 'cytology' & lines & refs - these were all unaltered from FB *F about a year ago. *F Ignore the .* suffixes on EST names. *F MA *F ========================================================================== *F ========================================================================== *F Region 34-36 genes. *F Michael Ashburner and John Roote and Sima Misra and Suzanna Lewis and *F Gerry M. Rubin. *F Version 18.1 *F 13 Sept. 1999 *F \+symbol = identified on sequence *F direction = direction of transcription *F + = distal to proximal *F - = proximal to distal *F ? = unclear since only P insertion *F product - often very very tentative *F BLAST results: expect < e-7 *F GB = GenBank/dbEST TBLASTX *F SP = SwissProt BLASTX *F TR = trEMBL BLASTX *F YPD = yeast protein database BLASTX *F genefinder scores > 20 *F genscan scores > 45 *F If only part of gene prediction used, indicated number of spans. *F \* = split gene prediction between 2 gene records. *F Results in parentheses less than the above cutoffs. *F =========================================================================== *F \+symbol: B4 *F \+formal_name: BG:DS07660.4 *F references: *F Sotillos et al., 1997 Development 124: 4769-4779 *F cDNA_sequence: AF022364; g2636727 *F pr_sequence_TREMBL:O16865 *F P_element_allele: *F PZ05337 sequenced *F EP2503 sequenced *F genefinder: 48(BACR48E02)/21(BACR48E02) *F genscan: 153(BACR48E02) *F product: *F match_to: SP P46591 e-9 O (Candida albicans) *F match_to: SP P08399 e-7 M (serine residues) *F EST: LD07105.5', LD33628.5' *F BAC: BACR48E02 *F direction: - *F MA_notes: This is a divergent transcript from kuz encoding a 964 aa protein *F with *F no matches. 5' ends of anon-B4 and kuz a few kb apart Sotillos et al., 1997. *F SM_notes: Translation from alignment to GenBank AF022364 (only 1-2314 of *F 4053). n(2)05337 maps to 187441 (in 4th exon), EP2503 maps to 151575 of *F BACR48E02 (in 1st exon). *F =========================================================================== *F \+symbol: BG:BACR48E02.1 *F \+formal_name: BG:BACR48E02.1 *F genefinder: 21 *F genscan: (31) *F product: *F match_to: *F EST: *F BAC: BACR48E02 *F direction: - *F Nested_in: B4 *F SM_notes: Translation from Genefinder. *F =========================================================================== *F \+symbol: BG:BACR48E02.2 *F \+formal_name: BG:BACR48E02.2 *F genefinder: (15) *F genscan: 63 *F product: *F match_to: *F EST: *F BAC: BACR48E02 *F direction: + *F Nested_in: B4 *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:BACR48E02.3 *F \+formal_name: BG:BACR48E02.3 *F genefinder: 23 *F genscan: 54 *F product: *F match_to: *F EST: *F BAC: BACR48E02 *F direction: + *F Nested_in: B4 *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: kuz *F \+formal_name: BG:DS07660.3 *F synonym: l(2)34Da *F synonym: l(2)03782 *F name: kuzbanian *F UID: FBgn0015954 *F references: *F FBrf0089802 == Rooke et al., 1996, Science 273(5279): 1227--1231 *F FBrf0090545 == Fambrough et al., 1996, Proc. Natl. Acad. Sci. USA. *F 93(23): 1 *F 3233--13238 *F Sotillos et al., 1997 Development 124: 4769-4779 *F cytology: 34C5--34C5 *F cytology_data: Left limit from inclusion within Df(2L)b88c75 (FBrf0092813) *F cytology_data: Right limit from in situ hybridisation (FBrf0067338) *F cDNA_sequence:U60591; g1531633 *F pr_sequence_TREMBL:Q94902 *F genefinder: 23/77(BACR48E02) *F genscan: 286/83(2 spans)(BACR48E02) *F product: metalloprotease/disintegrin family protein *F match_to: TR G2739037 (AF024614) e-128 W *F match_to: TR E1246683 (Z96047) e-128 W *F match_to: TR O14672 (AF009615) e-113 H *F match_to: TR O35598 (AF011379) e-112 M *F EST: LD04086.5', LD20268.5', LD04356.5' *F BAC: BACR48E02 *F direction: + *F TE_insert_allele: *F kuz1 (TE13=TE34Cb) *F kuz2 (TE60=TE34Ca) *F kuz3 (TE94=TE34Cc) *F P_element_allele: *F kuzk01403 sequenced l(2)k01405, cluster mate ? *F kuzk07601 sequenced *F kuzk12004 sequenced *F kuzk14817 sequenced *F kuzk09934 *F kuzk11804 *F kuzk14701 *F l(2)0378203782 (hypomorphic allele) sequenced *F rK028 sequenced *F P_element_derivative_allele: *F kuze29-4 *F kuzk01405Rev4 *F kuzk01405Rev11 *F kuzk01405Rev23 *F aberration_associated_allele: *F kuzTE34Cc-GV2 (In(2)TE34Cc-2) *F kuzTE34Cc-SV1 (In(2L)TE34Cc-1) *F kuzTE34Cc-GV1 (T(2;3)TE34Cc-1) *F kuzTE34Ca-GV1 (T(Y;2)TE34Ca-1) *F misc_allele: *F kuzIK5 *F kuzH143 *F phenotypic_class: vital. *F SM_notes: Translation from alignment to GenBank U60591 (edited 3' end of *F 2nd exon). *F rK028 maps to 151448 of BACR48E02. *F =========================================================================== *F \+symbol: BG:DS07660.1 *F \+formal_name: BG:DS07660.1 *F genefinder: 20(BACR48E02) *F genscan: 99(BACR48E02) *F product: Na-dependent transporter *F match_to: TR Q94886 (Y07720) e-56 D *F match_to: SP Q03567 e-72 W *F match_to: TR Q62634 (U07609) e-60 M *F match_to: SP Q61983 e-55 M *F match_to: SP Q14916 e-44 H *F match_to: TR G3582333 (AC005496) e-57 A *F match_to: LP07553.5' e-42 D *F EST: *F BAC: BACR48E02 *F direction: - *F Nested_in: kuz *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:BACR48E02.4 *F \+formal_name: BG:BACR48E02.4 *F genefinder: 42 *F genscan: 68 *F product: ras suppressor protein rsp-1 *F match_to: SP Q15404 e-69 H *F match_to: SP Q01730 e-68 M *F match_to: TR Q09497 (Z46996) e-58 W *F match_to: TR E1226129 (Y16045) e-15 A *F EST: LD13077.5', LD11984.5', LD18845.5', LP02171.5' *F BAC: BACR48E02 *F direction: - *F SM_notes: Translation from full-length LD13077. *F =========================================================================== *F \+symbol: BG:DS01368.1 *F \+formal_name: BG:DS01368.1 *F genefinder: 80(DS01368) *F genscan: 153(DS01368) *F product: *F match_to: TR Q18202 e-13 W *F EST: CK00026.3', LP04233.5' *F P1: DS01368 *F direction: + *F SM_notes: Translation from full-length CK00026. CK00026.3' matches but *F CK00026.5prime.contig sequence does not match; only nts 612-2123 of 2131 *F CK00026 full-length sequence align by sim4. *F LP04233.5' looks alternatively spliced, overlaps exon 4 and 6, not 5. *F =========================================================================== *F \+symbol: BG:DS08249.1 *F \+formal_name: BG:DS08249.1 *F genefinder: 36 *F genscan: 139(DS01368)/103(DS08249) *F product: similar to lachesin? *F match_to: SP Q26474 e-9 O (grasshopper) *F EST: *F P1: DS01368, DS08249, BACR48E02 *F direction: - *F SM_notes: Translation from Genscan on DS01368. match_to: GB Z81045;g1627647 *F e-8 W but by 1/99 this protein in the cosmid accession no longer exists. *F =========================================================================== *F \+symbol: BG:DS08249.2 *F \+formal_name: BG:DS08249.2 *F genefinder: 73 *F genscan: 163 *F product: mitochondrial glycerol 3-phosphate dehydrogenase *F match_to: GB U12424 e-87 H *F match_to: SP P43304 e-97 H *F match_to: GB U60987 e-87 M *F match_to: SP Q64521 e-100 M *F match_to: TR E1323155 e-97 W (EMBL; Z72516) *F match_to: GB Z73906 e-67 W *F match_to: TR P90795 e-78 W *F match_to: YPD 763191 e-61 Y *F match_to: SP P32191 e-52 Y *F EST: *F P1: DS08249 *F direction: + *F SM_notes: Genefinder used rather than Genscan for translation since Genscan *F adds *F on stuff at amino terminus. *F =========================================================================== *F \+symbol: BG:DS08249.3 *F \+formal_name: BG:DS08249.3 *F genefinder: 50 *F genscan: 91 *F product: *F match_to: TR G3342562 e-72 H (EMBL; AF077599) *F match_to: GB AA305709 e-29 H (Hum EST) *F match_to: GB AA212211 e-13 M (Mus EST) *F match_to: LD19320.5' e-16; LD28635.5' e-16 *F EST: *F P1: DS08249 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS08249.4 *F \+formal_name: BG:DS08249.4 *F genefinder: 38 *F genscan: 97 *F product: *F match_to: *F EST: *F P1: DS08249 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS08249.5 *F \+formal_name: BG:DS08249.5 *F genefinder: (18) *F genscan: 50 *F product: *F match_to: *F EST: *F P1: DS08249 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS00797.1 *F \+formal_name: BG:DS00797.1 *F synonym: l(2)k07245 *F UID: FBgn0022093 *F genefinder: 59 *F genscan: 185 *F product: similar to Yeast EMP70 (70kd endomembrane) gene product P24A *F protein precursor *F match_to: GB D87444 e-159 H (KIAA0255) *F match_to: TR Q92544 e-210 H (KIAA0255) *F match_to: TR Q94422 e-180 W *F match_to: TR E1247604 e-180 W *F match_to: TR Q12101 e-103 Y *F match_to: SP P32802 e-102 Y *F match_to: GB X67316 e-50 Y *F match_to: GB AA272456 e-37 M (Mus EST) *F match_to: GB AA117787 e-35 M (Mus EST) *F match_to: TR O04091 e-73 A *F match_to: GB T45159 e-24 A *F EST: HL07777.5'; HL07777.3'; LD32761.5'; GH02822.5' *F P_element_allele: *F l(2)k07245 sequenced *F P1: DS08284 *F direction: + *F SM_notes: k07245 inserted at 7502 of DS08284, LD32671.5' is from 7511-8360, *F extends further 5' than HL07777. Translation from full-length LD32761 sequence. *F =========================================================================== *F \+symbol: BG:DS00797.2 *F \+formal_name: BG:DS00797.2 *F genefinder: 39 *F genscan: 77 *F product: *F match_to: YPD 854436 e-16 Y *F match_to: GB C90076 e-15 O (Dictyostelium discoideum) *F EST: HL03175.5'; HL03175.3' *F P1: DS08284 *F direction: - *F SM_notes: Translation from full-length HL03175 sequence. *F =========================================================================== *F \+symbol: p38b *F \+formal_name: BG:DS00797.3 *F genefinder: 71 *F genscan: 173 *F product: MAP Kinase *F match_to: GB L35253 e-115 H *F match_to: GB L35264 e-115 H *F match_to: SP Q16539 e-136 H *F match_to: GB U10871 e-116 M *F match_to: SP P47811 e-136 M *F match_to: TR Q17466 e-116 W *F match_to: SP P32485 e-92 Y *F match_to: SP Q39026 e-86 A *F EST: GM01004.3'; GM01004.5'; GM04790.5'; GM06395.5'; HL03933.5'; LD24658.5' *F P1: DS08284 *F direction: + *F SM_notes: Translation from full-length GM01004. *F =========================================================================== *F \+symbol: BG:DS00797.4 *F \+formal_name: BG:DS00797.4 *F genefinder: 30 *F genscan: 69 *F product: *F match_to: TR Q17843 e-31 W *F match_to: GB Z81275 e-24 O (Scrofa EST) *F match_to: GB AA114025 e-19 H (Hum EST) *F match_to: GB R90322 e-16 A (Arabidopsis EST) *F EST: *F P1: DS08284 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS00797.5 *F \+formal_name: BG:DS00797.5 *F genefinder: 87 *F genscan: 296 *F product: ABC transporter *F match_to: GB X97187 e-82 H *F match_to: TR Q92473 e-102 H *F match_to: TR Q99758 e-102 H *F match_to: GB AF000149 e-71 M *F match_to: SP P41233 e-86 M *F match_to: TR O01790 e-69 W *F match_to: TR O22231 e-58 A *F match_to: SP P55476 e-31 B *F EST: CK01017.3'; CK01017.5'; LD33192.5' *F P1: DS08284 *F direction: + *F SM_notes: Translation from complete sequence of CK01017 at 5' end and *F LD33192 at 3' end--neither full-length. Because homology extends 5' of *F CK01017 and this library not full-length clones, gene may extend further 5' *F than translation. *F =========================================================================== *F \+symbol: BG:DS00797.6 *F \+formal_name: BG:DS00797.6 *F genefinder: 71 *F genscan: 170 *F product: *F match_to: TR Q25239 e-10 O (Lucilia cuprina) *F EST: *F P1: DS08284 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS00797.7 *F \+formal_name: BG:DS00797.7 *F genefinder: 218 *F genscan: 632 *F product: guanine nucleotide-exchange protein (SEC7) *F match_to: GB J03918 e-161 Y *F match_to: SP P11075 e-178 Y *F match_to: TR G2674107 0.0 O (Bos taurus) *F match_to: TR E1287857 e-181 A *F match_to: TR Q92538 e-51 H *F match_to: GB M85169 e-42 H *F match_to: TR O08967 e-44 M *F match_to: SP P34512 e-25 W *F EST: LD10472.3'; LD10472.5'; HL01681.5'; LD29171.5' *F P1: DS08284 *F direction: + *F MA_note: Bet this is anon-34Da *F SM_notes: Translation from full-length LD29171. *F =========================================================================== *F \+symbol: BG:DS00941.1 *F \+formal_name: BG:DS00941.1 *F genefinder: 56 *F genscan: 122 *F product: carbonic anhydrase *F match_to: GB U55177 e-50 O (Danio rerio) *F match_to: TR G2576335 e-53 O (Danio rerio) *F match_to: SP P23589 e-47 M *F match_to: GB X51971 e-40 M *F match_to: SP P43166 e-50 H *F match_to: GB L19297 e-40 H *F match_to: TR Q21614 e-28 W *F match_to: GB Z68118 e-14 W *F match_to: TR O04846 e-7 A *F EST: GH09688.5'; GH05601.3'; GH06111.3'; GH08627.5'; GH08470.5'; GH05849.5' *F P1: DS00941 *F direction: + *F SM_notes: Translation from full-length GH09688, but problem aligning *F 1321-2642 (internal exon) of GH09688; GH05601.3' and GH05601.3' align *F there so used these for middle of 3rd exon. *F =========================================================================== *F \+symbol: BG:DS00941.2 *F \+formal_name: BG:DS00941.2 *F genefinder: 55 *F genscan: 54 *F product: RNA editase *F match_to: GB X99383 e-23 H *F match_to: GB U76420 e-23 H *F match_to: SP P55265 e-18 H *F match_to: TR G2981097 e-16 M *F match_to: SP Q22618 e-15 W *F match_to: SP P53065 e-12 Y *F EST: *F P1: DS00941 *F direction: - *F SM_notes: Translation from Genscan. Missing key dsRNA binding domains. *F MA_notes: Liam Keegan (Edinburgh) named it Adat and think it is a *F tRNA adenosine deaminase *F =========================================================================== *F \+symbol: BG:DS00941.3 *F \+formal_name: BG:DS00941.3 *F genefinder: 44 *F genscan: 99 *F product: *F match_to: GB D76471 e-7 M (Mus EST) *F match_to: TR G2772565 e-12 H (Hum EST) *F EST: *F P1: DS00941 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F symbol: l(2)34Db *F name: lethal (2) 34Db *F UID: FBgn0001959 *F references: *F cytology: 34D3--34D3 *F cytology_data: Left limit from inclusion within Df(2L)b81a1 (FBrf0091422) *F cytology_data: Right limit from complementation mapping against In(2L)tsh3 *F (citation unavailable) *F ems_allele: *F l(2)34Db1 *F l(2)34Db2 *F l(2)34Db3 *F l(2)34Db4 *F l(2)34Db5 *F l(2)34Db6 *F l(2)34Db7 *F l(2)34Db8 *F l(2)34Db9 *F l(2)34Db10 *F l(2)34Db11 *F l(2)34Db12 *F misc_allele: *F l(2)34DbAS1 *F aberration_associated_allele: *F l(2)34Dbtsh3 (In(2L)tsh3) *F phenotypic_notes: escapers with rough eyes; etched wing margin; bristle *F loss etc; fertile both sexes. *F phenotypic_class: vital. *F JR_notes: OM's in situs of DS00797 suggest that this P1 is almost entirely *F distal to tsh3 break, ie that 34Db is probably either BG:DS00941.1, .2 or .3. *F One lethal excision (out of loads of viables) from k07245 is *F kuz-, 34Db+, Sos+, black+ i.e. 34Db is to the right of k07245. *F =========================================================================== *F symbol: anon-34Da *F synonym: transcript 7 *F references: *F FBrf0057637 == Bonfini et al., 1992, Science 255: 603--606 *F MA_note: Bet this is BG:DS00797.7 *F =========================================================================== *F \+symbol: Sos *F \+formal_name: BG:DS00941.4 *F name: Son of sevenless *F synonym: l(2)34Ea *F UID: FBgn0001965 *F references: *F FBrf0057637 == Bonfini et al., 1992, Science 255: 603--606 *F FBrf0053370 == Rogge et al., 1991, Cell 64: 39--48 *F FBrf0053392 == Simon et al., 1991, Cell 67: 701--716 *F FBrf0057544 == Bowtell et al., 1992, Proc. Natl. Acad. Sci. USA. 89: *F 6511--6515 *F FBrf0057536 == Rogge et al., 1992, Proc. natn. Acad. Sci. USA. 89: *F 5271--5275 *F cytology: 34D4--34D4 *F cytology_data: Left limit from complementation mapping against *F In(2LR)b71k1A (FBrf0091347) *F cytology_data: Right limit from non-inclusion within Df(2L)b77c (citation *F unavailable) *F product: guanine-nucleotide-exchange-factor *F motifs: PS00720 == Guanine-nucleotide dissociation stimulators CDC25 family *F signature. *F motifs: PS50003 == PH domain profile. *F genefinder: 230 *F genscan: 573 *F match_to: TR Q07889 e-254 H *F match_to: TR G303382 e-254 H *F match_to: TR Q62245 e-249 M *F match_to: GB L13857 e-232 H *F match_to: GB Z11574 e-229 M *F match_to: SP P04821 e-26 Y *F match_to: TR Q22503 e-14 W *F EST: GH01796.5' *F P1: DS00941 *F direction: + *F homology: species == Homo sapiens; gene == SOS2; GDB: 230041; OMIM: 601247 *F MEDLINE: 94102743 *F homology: species == Homo sapiens; gene == SOS1; GDB: 230004; OMIM: 182530 *F homology: species == Mus; gene == Sos1; MGD: MRK-14489 *F homology: species == Mus; gene == Sos2; MGD: MRK-14490 *F cDNA_sequence:M83931; g158485 *F genomic_sequence:M77501; g158471 *F pr_sequence_PIR:A41216 *F pr_sequence_SwissProt:P26675 *F ems_allele: *F Sos34Ea-1 *F Sos34Ea-2 *F Sos34Ea-3 *F Sos34Ea-4 *F Sos34Ea-5 *F Sos34Ea-6 *F Sos34Ea-7 *F Sos34Ea-8 *F Sos34Ea-9 *F Sos34Ea-10 *F Sos34Ea-11 *F Sos34Ea-12 *F Sos34Ea-13 *F Sos34Ea-14 *F Sos34Ea-15 *F Sos34Ea-16 *F Sos34Ea-17 *F Sos34Ea-18 *F Sos34Ea-19 *F SosF1 *F SoseCN6 *F SoseCS3 *F SoseDN4 *F SoseJ2 *F SoseN6 *F P_element_allele: *F E(var)3091 *F E(var)1891 *F l(2)k05224 sequenced *F l(2)k06321 *F l(2)k05705 *F misc_allele: *F SosJC2 *F SosSutor-157 *F SosSutor-405 *F Sose0D *F Sose1A *F Sose2H *F Sose4G *F Sose6C *F Sosex63 *F Sosx122 *F Sosx15 *F phenotypic_class: vital. *F phenotypic_notes: escapers with small rough eyes; wing vein gaps. *F SM_notes: Translation from alignment of GenBank M83931. *F =========================================================================== *F \+symbol: b *F \+formal_name: BG:DS00941.5 *F name: black *F synonym: Gad2 *F synonym: anon-34Db *F synonym: transcript 1 Bonfini *F UID: FBgn0000153 *F UID: FBgn0005622 *F references: *F FBrf0064653 == Phillips et al., 1993, J. Neurochem. Suppl. 61(4): *F 1291--1301 *F FBrf0082478 == Phillips et al., 1995, Abstr. Europ. Symp. Dros. *F Neurobiol. 5: 44--45 *F FBrf0053317 == Phillips et al., 1991, Greenspan, Palka, 1991: 97 *F cytology: 34D6--34D6 *F cytology_data: Left limit from complementation mapping against *F T(2;3)b79b6 (citation unavailable) *F cytology_data: Right limit from complementation mapping against *F T(2;3)b79b6 (citation unavailable) *F enzyme: glutamate decarboxylase == EC 4.1.1.15 *F cDNA_sequence:U01239; g402252 *F pr_sequence_TREMBL:Q24062 *F pr_sequence_PIR:JH0827 *F genefinder: 109 *F genscan: 259 *F match_to: GB X69936 e-109 H *F match_to: SP Q05329 e-115 H *F match_to: TR Q64611 e-127 O (Rattus norvegicus) *F match_to: TR O35119 e-114 M *F match_to: SP P48320 e-114 M *F match_to: GB L16980 e-110 M *F match_to: GB Z14925 e-14 W *F EST: HL08051.5'; HL07852.5' *F P1: DS00941 *F direction: + *F spontaneous_allele: *F b1 *F b14b28 *F b18c27 *F b23b2 *F b24b15 *F b29 *F b34g *F b39 *F b39j *F b40c *F b82c1 *F UV_allele: *F b50d *F b51f *F gamma_ray_allele: *F b661 *F b71k2 *F b75a *F b76e1 *F b76e2 *F b76f3 *F b77j *F b78a *F b79b1 *F b79d2 *F b79df2 *F b79dt3 *F b79g2 *F b79h2 *F b79h3 *F b80c1 *F b80i2 *F b80i3 *F b80j1 *F b80j2 *F b80k1 *F b80k2 *F b80l1 *F b80l2 *F b81a2 *F b81c17 *F b81c2 *F b81f2 *F b81h2 *F b81k *F b81l2 *F b81l40 *F b81l42 *F b83b1 *F b83b40 *F b83c20 *F b83c25 *F b83c26 *F b83c35a *F b83c35b *F b83c36 *F b83c47 *F b83f17 *F b83f18 *F b83f51 *F b83f52 *F b84b1 *F b84h70 *F b85b3 *F b85b4 *F caffeine/gamma_ray_allele: *F b74b2 *F b74b4 *F b74b5 *F b74c2 *F b74c4 *F b74c5 *F b74d2 *F b74d4 *F b74d6 *F b76b1 *F b76b2 *F actinomycin_D/gamma_ray_allele: *F b76j1 *F b76j2 *F b76j3 *F b76k1 *F b76k2 *F b77a1 *F b77a2 *F b77a3 *F b77a4 *F b77a5 *F NaF/gamma_ray_allele: *F b78f1 *F b78f2 *F b78g *F b78k1 *F b78k2 *F b78k3 *F b78k4 *F b78k5 *F neutrons/gamma_ray_allele: *F b79d6 *F b79d8 *F b79dt0 *F X_ray_allele: *F b77.1X *F b77.2X *F b77.5X *F b83fXD *F b84g *F b84h34 *F b84h40 *F neutron_allele: *F b79a1 *F b79a2 *F b79a3 *F b79b7 *F b79d4 *F b79dt1 *F b81c *F b82c16 *F b82c3 *F b82c54 *F b82c7 *F b83d29b *F b83d35 *F b83d36 *F b83l *F b85c3 *F ems_allele: *F b75f *F b77.3e *F b77.4e *F bA *F bA51 *F bA53 *F bA54 *F bA55 *F diepoxyoctane_allele: *F b78.1 *F misc_allele: *F b66h *F b81dt *F P_element_allele: *F bP *F aberration_associated_allele: *F b36f (Tp(2;3)dp) *F b79b6 (T(2;3)b79b6) *F b79d5 (In(2L)b79d5) *F b79d6 (T(2;3)b79d6) *F b79h1 (Tp(2;2)b79h1) *F b80c2 (In(2L)b80c2) *F b81a (In(2LR)b81a2) *F b81f1 (Df(2L)b81f1) *F b81f3 (In(2L)b81f3) *F b81l17 (In(2L)b81l7) *F b82c44 (In(2L)b82c44) *F b83b2 (In(2L)b83b22) *F b85c2 (T(2;3)b85c2) *F phenotypic_notes: black body color; puparial case light; lacks *F beta-alanine; presumably on beta-alanine pathway. *F phenotypic_class: visible; non_vital. *F misc_note: if GAD acts as an aspartate decarboxylase then b = Gad2. *F JR_notes: Marie Phillips (Melbourne) wrote (1991) that Gad2 was in *F Df(2L)b84a4 but not in Df(2L)b84a8. *F SM_notes: Translation from alignment of GenBank U01239. *F =========================================================================== *F \+symbol: tamas *F \+formal_name: BG:DS00941.6 *F name: lethal (2) 34Dc *F synonym: DNApol-&ggr;125 *F synonym: l(2)34Dc *F synonym: anon-34Dc *F synonym: transcript 2 Bonfini *F UID: FBgn0001960 *F UID: FBgn0004406 *F references: *F FBrf0090361 == Lewis et al., 1996, J. Biol. Chem. 271(38): 23389--23394 *F FBrf0092749 == Ropp and Copeland, 1996, Genomics 36(3): 449--458 *F FBrf0089488 == Ropp, 1996.9.23, personal communication *F FBrf0056420 == Olson and Kaguni, 1992, J. Biol. Chem. 267: 23136--23142 *F FBrf0050857 == Kaguni and Olson, 1989, Proc. natn. Acad. Sci. USA. *F 86(17): 6469--6473 *F FBrf0047578 == Wernette et al., 1988, Biochemistry 27: 6046--6054 *F FBrf0044677 == Wernette and Kaguni, 1986, J. Biol. Chem. 261(31): *F 14764--14770 *F FBrf0063558 == Kaguni et al., 1988, Cancer Cells 6: 425--432 *F FBrf0064748 == Williams et al., 1993, J. Biol. Chem. 268(33): 24855--24862 *F FBrf0079622 == Williams and Kaguni, 1995, J. Biol. Chem. 270(2): 860--865 *F FBrf0085690 == Olson et al., 1995, J. Biol. Chem. 270(48): 28932--28937 *F FBrf0090538 == Dusenbery and Smith, 1996, Mutat. Res. 364(3): 133--145 *F cytology: 34D6--34D8 *F cytology_data: Left limit from complementation mapping against *F In(2L)b79d5 (citation unavailable) *F cytology_data: Right limit from inclusion within Df(2L)b82a3 (citation *F unavailable) *F enzyme: mitochondrial DNA-directed DNA polymerase, catalytic subunit == EC *F 2.7.7.7 *F genefinder: 143 *F genscan: 337 *F match_to: TR Q92515 e-232 H *F match_to: GB U60325 e-215 H *F match_to: GB X98093 e-215 H *F match_to: SP P54099 e-216 M *F match_to: GB U53584 e-203 M *F match_to: SP P15801 e-129 Y *F match_to: GB Z75238 e-104 Y *F match_to: GB D35928 e-10 W *F EST: LD06421.5'; LD18609.5'; LD02053.5' *F P1: DS00941 *F direction: - *F genomic_sequence:U60298; g1401344 *F cDNA_sequence:U62547; g1552493 *F pr_sequence_SwissProt:Q27607 *F pr_sequence_TREMBL:Q94906 *F ems_allele: *F l(2)34Dc1 *F l(2)34Dc2 *F l(2)34Dc3 *F l(2)34Dc4 *F l(2)34Dc5 *F l(2)34Dc6 *F l(2)34Dc7 *F l(2)34Dc8 *F phenotypic_class: vital. *F JR_notes: Ann Regina Campos (Ontario) picked up as mutation in adult/larval *F visual system; B. Iyengar *F SM_notes: Translation from alignment of GenBank U62547. *F =========================================================================== *F \+symbol: Sop2 *F \+formal_name: BG:DS00941.7 *F name: Suppressor of profilin *F synonym: l(2)34Dd *F UID: FBgn0001961 *F references: *F to JR 13 March 1997. *F cytology: 34D6--34D8 *F cytology_data: Left limit from complementation mapping against In(2L)b79h1A *F (citation unavailable) *F cytology_data: Right limit from inclusion within Df(2L)b82a3 (citation *F unavailable) *F homology: species == S. pombe; gene == sop2 EMBO J. 15: 6426 *F genefinder: 75 *F genscan: 199 *F match_to: TR Q92747 e-106 H *F match_to: GB Y08999 e-102 H *F match_to: GB Z36103 e-59 Y *F match_to: SP P38328 e-60 Y *F match_to: GB W08597 e-49 M (Mus EST) *F match_to: GB AA408535 e-49 M (Mus EST) *F match_to: GB C12211 e-36 W *F EST: CK00388.3' *F P1: DS00941 *F direction: - *F ems_allele: *F l(2)34Dd1 *F l(2)34Dd2 *F l(2)34Dd3 *F phenotypic_notes: interallelic escapers. *F phenotypic_class: vital. *F JR_notes: Information on product & orders from Andrew Hudson (chez Cooley). *F SM_notes: Translation from alignment of cDNA from A. Hudson (from GM library). *F =========================================================================== *F \+symbol: Orc5 *F \+formal_name: BG:DS00941.8 *F name: Origin recognition complex subunit 5 *F synonym: l(2)34Df *F UID: FBgn0001963 *F references: *F FBrf0085120 == Gossen et al., 1995, Science 270(5242): 1674--1677 *F FBrf0086393 == Ehrenhofer-Murray et al., 1995, Science 270(5242): *F 1671--1674 *F cytology: 34D6--34E2 *F cytology_data: Left limit from non-inclusion within Df(2L)b88b42 (citation *F unavailable) *F cytology_data: Right limit from inclusion within Df(2L)b83l1 (citation *F unavailable) *F product: origin recognition complex subunit *F genefinder: 59 *F genscan: 166 *F match_to: TR G2906228 e-58 H *F match_to: TR G2739446 e-58 H *F EST: *F P1: DS00941 *F direction: + *F cDNA_sequence:U43505; g1136136 *F pr_sequence_SwissProt:Q24169 *F ems_allele: *F l(2)34Df1 *F l(2)34Df2 *F phenotypic_class: vital. *F JR_notes: Information on Orc5 from Michelle Pflumme (chez Mike Botchan). *F SM_notes: Translation from alignment of GenBank U43505. *F =========================================================================== *F \+symbol: MtPolB *F \+formal_name: BG:DS00941.9 *F synonym: l(2)34De *F UID: FBgn0020398 *F UID: FBgn0001962 *F cytology: 34D8--34E1 *F cytology_data: Left limit from non-inclusion within Df(2L)b85b2 (citation *F unavailable) *F cytology_data: Right limit from non-inclusion within Df(2L)A47 (FBrf0038050) *F references: *F FBrf0093778 == Wang et al., 1997, J. Biol. Chem. 272(21): 13640--13646 *F cDNA_sequence:U94702; g2114434 *F pr_sequence_TREMBL:O02005 *F enzyme: mitochondrial DNA-directed DNA polymerase, accessory subunit == EC *F 2.7.7.7 *F genefinder: 25/26 *F genscan: 86 *F match_to: SP P54098 e-231 H *F match_to: TR Q92515 e-232 H *F EST: GM03048.3'; GM03048.5' *F P1: DS00941 *F direction: - *F ems_allele: *F l(2)34De1 *F l(2)34De2 *F l(2)34De3 *F phenotypic_class: vital. *F JR_notes: Identity of 34De and MtPolB based on mapping with b89e12 ordering *F 34De and 34Dg. *F SM_notes: Translation from alignment of GenBank U94702. *F =========================================================================== *F \+symbol: RpII33 *F \+formal_name: BG:DS00941.10 *F name: lethal (2) 34Dg *F synonym: l(2)34Dg *F UID: FBgn0001964 *F references: *F cytology: 34D8--34D8 *F cytology_data: Left limit from non-inclusion within Df(2L)b85b2 (citation *F unavailable) *F cytology_data: Right limit from inclusion within Df(2L)b81l42 (citation *F unavailable) *F product: RNA polymerase II 33 KD subunit *F enzyme: DNA-directed RNA polymerase == EC 2.7.7.6 *F genefinder: 53 *F genscan: 161 *F match_to: GB AF008443 e-105 H *F match_to: TR O15161 e-107 H *F match_to: SP P19387 e-106 H *F match_to: GB D83999 e-100 M *F match_to: SP P97760 e-101 M *F match_to: TR Q93338 e-82 W *F match_to: GB Z81079 e-41 W *F match_to: GB M27496 e-53 Y *F match_to: SP P16370 e-56 Y *F match_to: TR Q39211 e-45 A *F EST: LD09978.3'; LD09978.5'; LD05121.5'; LD08178.5' *F P1: DS00941 *F direction: + *F ems_allele: *F l(2)34Dg1 *F l(2)34Dg2 *F P_element_allele: *F l(2)k05605 sequenced *F phenotypic_class: vital. *F JR_notes: Information on product & orders from Andrew Hudson (chez Cooley). *F SM_notes: Translation from full-length LD09978. *F =========================================================================== *F \+symbol: BG:DS00941.11 *F \+formal_name: BG:DS00941.11 *F genefinder: 47 *F genscan: 98 *F product: *F match_to: *F EST: *F P1: DS00941 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS00941.12 *F \+formal_name: BG:DS00941.12 *F genefinder: 58 *F genscan: 139 *F product: *F match_to: *F EST: *F P1: DS00941 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS00941.13 *F \+formal_name: BG:DS00941.13 *F genefinder: 50 *F genscan: 113 *F product: *F match_to: *F EST: LP05416.5' *F P1: DS00941 *F direction: + *F SM_notes: Translation from full-length LP05416. *F ========================================================================== *F \+symbol: BG:DS00941.14 *F \+formal_name: BG:DS00941.14 *F genefinder: 65 *F genscan: 145 *F product: *F match_to: *F EST: LP05733.5', LP01570.5', LP03350.5' *F P1: DS00941 *F direction: + *F SM_notes: Translation from full-length LP05733. *F ========================================================================== *F \+symbol: BG:DS00941.15 *F \+formal_name: BG:DS00941.15 *F genefinder: 32 *F genscan: (43) *F product: *F match_to: *F EST: LP04791.5' *F P1: DS00941 *F direction: + *F SM_notes: Translation from LP04791.5' at 5' end and Genscan at 3' end. *F ========================================================================== *F \+symbol: BG:DS08220.1 *F \+formal_name: BG:DS08220.1 *F P_element_allele: *F PZ06646 sequenced *F rN149 sequenced *F ms(2)k10802 sequenced not male-sterile *F genefinder: 127(DS08220) *F genscan: (22)(DS00941)/298(DS08220) *F product: *F match_to: GB D79989 e-103 H (KIAA0167) *F match_to: TR O00578 e-114 H *F match_to: GB C08626 e-16 W (worm EST) *F match_to: GB C08427 e-14 W (worm EST) *F match_to: TR O04097 e-11 A *F match_to: GB T04032 e-9 A *F EST: LD11783.5'; LD11783.3'; GM01069.5'; GM01069.3' *F P1: DS00941, DS08220 *F direction: + *F JR_notes: all excisions of 06646 viable; 2/44 excisions of rN149 are lethal *F but lethality not to this region (being mapped by JR). *F SM_notes: Translation from full-length LD11783. n(2)rN149 and PZ06646 map *F to ex *F actly the same nucleotide with the *F same orientation, 4552 of DS00941 (d>p revcomp 76272 of DS00941); *F ms(2)k10802 maps at 3710 of DS00941. *F LD11783.5' is from 3699-3125 (d>p revcomp 77325-77899), 1kb downstream of *F rN149/PZ06646, and 11bp downstream of ms(2)k10802. *F ========================================================================== *F \+symbol: anon-34Ea *F \+formal_name: BG:DS08220.2 *F references: *F FBrf0083469 == Tatei et al., 1995, Mech. Dev. 51(2-3): 157--168 *F P_element_allele: *F EP(2)2171 sequenced *F genefinder: 175 *F genscan: 505 *F product: *F match_to: *F EST: LD02558.3'; LD02558.5'; LD06162.3'; LD06162.5'; LD20278.5'; *F LD12350.5'; LD22235.5'; LD29084.5' *F P1: DS08220 *F direction: - *F misc_data: 16.5kb EcoRI fragment including the Ance and anon-34Ea genes. *F SM_notes: Translation from full-length LD02558. EP(2)2171 is inserted at *F 3159 of DS08220, LD29084.5' first exon *F is 3139-3164, so P is 5bp upstream of first intron. *F =========================================================================== *F \+symbol: Ance *F \+formal_name: BG:DS08220.3 *F synonym: l(2)34Eb *F name: Angiotensin converting enzyme *F UID: FBgn0012037 *F references: *F FBrf0081664 == Cornell et al., 1995, J. Biol. Chem. 270(23): 13613--13619 *F FBrf0083469 == Tatei et al., 1995, Mech. Dev. 51(2-3): 157--168 *F FBrf0087131 == Frank and Rushlow, 1996, Development 122(5): 1343--1352 *F FBrf0090455 == Biehs et al., 1996, Genes Dev. 10(22): 2922--2934 *F FBrf0092672 == Rusch and Levine, 1997, Development 124(2): 303--311 *F cytology: 34E2--34E2+ *F cytology_data: Left limit from inclusion within Df(2L)Adh-nBR55 (FBrf0083469) *F cytology_data: Right limit from inclusion within Df(2L)b74c6 (citation *F unavailable) *F enzyme: peptidyl-dipeptidase A == EC 3.4.15.1 *F homology: species == Mus; gene == Ace; MGD: MRK-1032 *F homology: species == Homo sapiens; gene == DCP1; GDB: 119840; OMIM: 106180 *F homology: species == Homo sapiens; gene == DCP1; GDB: 119840; OMIM: 106180 *F genefinder: 41(DS08220)/107(DS00180) *F genscan: 114(DS08220)/296*(DS00180) *F match_to: SP Q10715 e-71(DS08220)/e-208(DS00180) O (Haematobia irritans) *F match_to: GB L43965 e-66(DS08220)/e-206(DS00180) O (Haematobia irritans) *F match_to: SP P22966 e-16(DS08220)/e-101(DS00180) H *F match_to: GB M26657 e-13(DS08220)/e-99(DS00180) H *F match_to: GB A31567 e-13(DS08220)/e-99(DS00180) H *F match_to: GB X16295 e-13(DS08220)/e-99(DS00180) H *F match_to: GB J04144 e-13(DS08220)/e-173(DS00180) H *F match_to: GB A00914 e-13(DS08220)/e-173(DS00180) H *F match_to: SP P22967 e-16(DS08220)/e-106(DS00180) M *F match_to: SP P09470 e-190(DS00180) M *F match_to: GB M55333 e-14(DS08220)/e-104(DS00180) M *F match_to: GB J04946 e-151(DS00180) M *F match_to: TR Q18581 e-26(DS00180) W *F EST: LD11258.5'; LD11258.3'; LD06090.5'; LD17344.5'; GM02190.5'; GM01822.5' *F P1: DS08220, DS00180 *F direction: + *F cDNA_sequence:U25344; g1373009 *F cDNA_sequence:U34599; g1002909 *F pr_sequence_SwissProt:Q10714 *F pr_sequence_PIR:A57533 *F ems_allele: *F Ance34Eb-1 *F Ance34Eb-2 *F phenotypic_class: vital. *F SM_notes: Translation of 3' from aligned GenBank U25344 on DS00180. *F =========================================================================== *F symbol: l(2)34Ec *F JR_notes: predicted by non rescue by Ance transgene of some Ance- o/laps. *F =========================================================================== *F \+symbol: Acyp *F \+formal_name: BG:DS00180.1 *F UID: FBgn0025115 *F references: *F \*x FBrf0104497 == Pieri et al., 1998, FEBS Lett. 433(3): 205--210 *F enzyme: acyl phosphatase == EC 3.6.1.7 *F genefinder: 20 *F genscan: 296* (one span) *F match_to: SP P56375 e-15 M *F match_to: SP P07311 e-15 H *F match_to: GB X84194 e-13 H *F match_to: GB X84195 e-13 H *F match_to: GB AA285985 e-13 M (mus EST) *F EST: *F P1: DS00180 *F direction: + *F pr_sequence_SwissProt:P56544 *F SM_notes: Translation from Genefinder. *F ========================================================================== *F \+symbol: BG:DS00180.2 *F \+formal_name: BG:DS00180.2 *F genefinder: 69 *F genscan: 184 *F product: *F match_to: TR Q39721 e-49 O (Euglena gracilis) *F match_to: TR Q22807 e-9 W *F EST: *F P1: DS00180 *F direction: - *F SM_notes: Translation from Genefinder. *F ========================================================================== *F \+symbol: BG:DS00180.3 *F \+formal_name: BG:DS00180.3 *F genefinder: 66 *F genscan: 142/145(2 spans) *F product: articulin *F match_to: TR Q39721 e-49 O (Euglena gracilis) *F match_to: SP Q02817 e-21 H *F match_to: YPD 763364 e-19 Y *F match_to: SP P15265 e-8 M *F EST: HL02234.5'; HL02234.3' *F P1: DS00180 *F direction: - *F SM_notes: Translation from full-length HL02234. *F ========================================================================== *F \+symbol: BG:DS00180.5 *F \+formal_name: BG:DS00180.5 *F genefinder: 23/35 *F genscan: 201*(9 spans) *F product: *F match_to: SP Q10715 e-203 O *F match_to: SP P09470 e-190 M *F match_to: SP P12821 e-187 H *F match_to: TR Q18581 e-8 W *F EST: *F P1: DS00180 *F direction: + *F SM_notes: Translation from edited Genscan. *F ========================================================================== *F \+symbol: BG:DS00180.12 *F \+formal_name: BG:DS00180.12 *F genefinder: 32*(3 spans) *F genscan: 201*(3 spans) *F product: *F match_to: SP Q61554 e-43 M *F match_to: SP P35555 e-41 H *F match_to: TR Q23587 e-22 W *F EST: GH08194.5'; GH07762.5' *F P1: DS00180 *F direction: + *F SM_notes: Translation from full-length GH07762. *F ========================================================================== *F \+symbol: BG:DS00180.7 *F \+formal_name: BG:DS00180.7 *F genefinder: 67 *F genscan: 100 *F product: tenascin X-like repeat *F match_to: SP P24821 e-30 H *F match_to: TR Q08424 e-44 H *F match_to: TR O35452 e-43 M *F match_to: SP P98133 e-37 O (cow) *F match_to: SP P14585 e-13 W *F match_to: TR O18375 e-29 D *F EST: HL01444.5'; HL01444.3' *F P1: DS00180 *F direction: - *F SM_notes: Translation from full-length HL01444 *F =========================================================================== *F \+symbol: BG:DS00180.8 *F \+formal_name: BG:DS00180.8 *F genefinder: 62 *F genscan: 128 *F product: tenascin-like repeat *F match_to: SP P24821 e-30 H *F match_to: TR Q08424 e-44 H *F match_to: TR O18375 e-29 D *F match_to: TR Q64706 e-26 M *F match_to: TR O01343 e-16 W *F EST: CK00536.5'; CK00194.5' *F P1: DS00180 *F direction: - *F SM_notes: Translation from full-length CK00536. *F =========================================================================== *F \+symbol: BG:DS00180.9 *F \+formal_name: BG:DS00180.9 *F genefinder: 30 *F genscan: 85 *F product: tenascin/Notch-like repeat *F match_to: SP P10039 e-31 O (chicken) *F match_to: TR D1013803 e-15 H *F match_to: SP Q61554 e-31 M *F EST: *F P1: DS00180 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS00180.10 *F \+formal_name: BG:DS00180.10 *F genefinder: 21 *F genscan: 120 *F product: Notch-like repeat *F match_to: SP Q01705 e-26 M *F match_to: TR Q23587 e-24 W *F match_to: TR O18375 e-29 D *F match_to: HL01967.5' D (Dros EST) *F EST: LP05465.5' *F P1: DS00180 *F direction: - *F SM_notes: Translation from 5'EST and Genscan. *F ========================================================================== *F \+symbol: BG:DS00180.11 *F \+formal_name: BG:DS00180.11 *F genefinder: 91*(5 spans) *F genscan: 217*(6 spans) *F product: cytochrome P450 *F match_to: TR G2673981 e-153 O (D.mettleri) *F match_to: TR Q64407 e-18 M *F match_to: SP P33268 e-16 H *F match_to: HL05525.5' D (Dros EST) *F EST: *F P1: DS00180 *F direction: + *F SM_notes: Translation from edited Genscan. Cyp28a5 67% identical to Cyp28a1 *F ========================================================================== *F \+symbol: BG:DS00180.14 *F \+formal_name: BG:DS00180.14 *F genefinder: 91*(2 spans) *F genscan: 217*(3 spans) *F match_to: TR Q15567 e-32 H *F match_to: TR O18375 e-32 D *F EST: *F P1: DS00180 *F direction: + *F SM_notes: Translation from edited Genscan. *F ========================================================================== *F symbol: j *F name: jaunty *F UID: FBgn0001277 *F references: *F cytology: 34E3--34E3 *F cytology_data: Left limit from inclusion within Df(2L)fn26 (FBrf0030161) *F cytology_data: Right limit from non-inclusion within Df(2L)A376 (FBrf0038050) *F spontaneous_allele: *F j2 *F j49j *F j50e *F j58i *F ems_allele: *F j63 *F jSF7 *F misc_allele: *F j1 *F phenotypic_notes: wings upturned. *F phenotypic_class: visible; non_vital. *F =========================================================================== *F \+symbol: rk *F \+formal_name: BG:DS00180.13 *F name: rickets *F UID: FBgn0003255 *F references: *F cytology: 34E4--34E5 *F cytology_data: Left limit from inclusion within Df(2L)fn12 (FBrf0030161) *F cytology_data: Right limit from inclusion within Df(2L)b-L (FBrf0036112) *F genefinder: 87/12(DS00180)/26(DS01514) *F genscan: 207(DS00180)/59(DS01514) *F product: lutropin/choriogonadotropin receptor *F match_to: TR U47005 e-77(DS00180) D *F match_to: TR Q94979 e-77(DS00180) D *F match_to: GB M73746 e-79(DS00180) H *F match_to: GB M63108 e-79(DS00180) H *F match_to: SP P22888 e-82(DS00180) H *F match_to: SP P30730 e-82(DS00180) M *F match_to: GB M81310 e-77(DS00180) M *F match_to: TR Q18759 e-41(DS00180) W *F match_to: GB Z73971 e-19(DS00180) W *F match_to: TR O15421 e-9(DS01514) H *F EST: *F P1: DS00180, DS01514 *F direction: - *F spontaneous_allele: *F rk4 *F rk5 *F rkcyl *F X_ray_allele: *F rk6 *F gamma_ray_allele: *F rk81f2 *F rk84b1 *F UV_allele: *F rk1 *F rk2 *F rk3 *F ems_allele: *F rk1250 *F rk76 *F rk80l1 *F rk80l2 *F rkEMS *F PM_dysgenesis_allele: *F rkKe1 *F P_element_allele: *F rk11P sequenced *F misc_allele: *F rkAS3 *F rkIK6 *F aberration_associated_allele: *F rknoc11 (Df(2L)noc11) *F phenotypic_notes: legs 'crippled'; tarsal segements short etc. *F phenotypic_class: visible; non_vital. *F JR_notes: James Baker (chez Jim Truman) suspects rk to be involved in *F control of eclosion behaviour by peptide hormones. *F SM_notes: Translation 5' from Genscan on DS01514, 3' from Genscan on *F DS00180. rk11P maps to 9522 of DS01514, sequence from Baker is from *F 5576-1. *F =========================================================================== *F \+symbol: BG:DS01514.3 *F \+formal_name: BG:DS01514.3 *F genefinder: *F genscan: 49 *F product: *F match_to: *F EST: *F P1: DS01514 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS01514.1 *F \+formal_name: BG:DS01514.1 *F genefinder: *F genscan: *F tRNAscan-SE: 69 *F product: transfer RNA:gln *F match_to: GB X68534 e-5 O (rat) *F EST: *F P1: DS01514 *F direction: - *F Nested_in: BG:DS01514.3 *F ========================================================================== *F \+symbol: BG:DS01514.2 *F \+formal_name: BG:DS01514.2 *F synonym: 2a9hom *F P_element_allele: *F k09909 sequenced *F genefinder: 93(DS01514)/93(DS00131) *F genscan: 210(DS01514)/230(DS00131) *F product: long chain fatty acid CoA-ligase *F match_to: SP Q10776 e-35(DS01514)/e-42(DS00131) B (Mycobacterium tuberculosis) *F match_to: TR O30039 e-34(DS01514)/e-40(DS00131) B (Archaeoglobus fulgidus) *F match_to: GB Z74020 e-22(DS01514)/e-37(DS00131) B (Mycobacterium tuberculosis) *F match_to: SP P33121 e-28(DS01514)/e-31(DS00131) H *F match_to: GB D10040 e-23(DS01514)/e-29(DS00131) H *F match_to: SP P41216 e-27(DS01514)/e-32(DS00131) M *F match_to: GB U15977 e-31(DS00131) M *F match_to: TR Q18660 e-20(DS01514)/e-21(DS00131) W *F match_to: TR Q20264 e-16(DS01514) W *F match_to: TR G2736373 e-24(DS00131) W *F match_to: SP P30624 e-23(DS01514) Y *F match_to: SP P39518 e-23(DS00131) Y *F match_to: TR O22898 e-25(DS01514)/e-25(DS00131) A *F EST: LD10778.5'; LD10778.3'; LD02830.5'; HL05723.5'; GM08372.5'; *F GM14009.5'; GH01357.5'; GH02862.5'; GH02258.5' *F P1: DS01514, DS00131 *F direction: + *F SM_notes: Translation from aligned cDNA sequence from M. Leptin (expressed *F in anterior mesoderm, later in hemocytes) on DS01514 (5') and DS00131 (3'). *F ========================================================================== *F \+symbol: l(2)34Fa *F \+formal_name: BG:DS05899.2 *F name: lethal (2) 34Fa *F UID: FBgn0001967 *F cytology: 34F2+--34F2+ *F cytology_data: Left limit from inclusion within Df(2L)Adh-nBR41 (FBrf0091422) *F cytology_data: Right limit from inclusion within Df(2L)b88f43 (FBrf0092813) *F genefinder: 37(DS00131)/37(DS05899) *F genscan: 84(DS00131)/57(DS05899) *F product: *F match_to: *F EST: *F P1: DS00131, DS05899 *F direction: - *F ems_allele: *F l(2)34Fa1 *F l(2)34FaCH59 *F P_element_allele: *F l(2)34Fak00811 sequenced *F EP(2)2418 sequenced *F aberration_allele: Df(2L)el20 (hypomorphic) *F phenotypic_class: vital. *F SM_notes: Translation from Genscan prediction on DS00131. *F =========================================================================== *F \+symbol: BG:DS05899.1 *F \+formal_name: BG:DS05899.1 *F synonym: 2a9 *F genefinder: 87 *F genscan: 252 *F product: long chain fatty acid CoA-ligase *F match_to: GB AL021957 e-46 O (Mycobacterium tuberculosis) *F match_to: TR E1253260 e-54 O (Mycobacterium tuberculosis) *F match_to: TR D1032567 e-108 H *F match_to: SP P41215 e-25 H *F match_to: SP P41216 e-24 M *F match_to: TR Q19339 e-19 W *F match_to: SP P39518 e-16 Y *F match_to: TR O23268 e-28 A *F EST: LD28132.5' *F P1: DS05899 *F direction: + *F SM_notes: Translation from aligned cDNA sequence from M. Leptin (expressed *F in mesoderm and later in fatbody). *F =========================================================================== *F \+symbol: BG:DS05899.7 *F \+formal_name: BG:DS05899.7 *F genefinder: *F genscan: 55 *F product: repeated Leucine-rich segment found in many proteins *F match_to: TR Q18902 e-27 W *F match_to: SP P35858 e-21 H *F match_to: SP P70389 e-21 M *F match_to: SP P36047 e-14 Y *F EST: *F P1: DS05899 *F direction: - *F SM_notes: Translation from Genscan prediction. *F ========================================================================== *F \+symbol: BG:DS05899.6 *F \+formal_name: BG:DS05899.6 *F genefinder: *F genscan: 46 *F product: *F match_to: *F EST: *F P1: DS05899 *F direction: - *F SM_notes: Translation from Genscan prediction. *F =========================================================================== *F \+symbol: BG:DS05899.3 *F \+formal_name: BG:DS05899.3 *F genefinder: 42 *F genscan: 106 *F product: *F match_to: SP Q61554 e-8 M (fibrillin precursor) *F match_to: SP P41990 e-7 W (Apx-1 precursor) *F EST: *F P1: DS05899 *F direction: + *F SM_notes: Translation from Genscan prediction. *F =========================================================================== *F \+symbol: BG:DS05899.5 *F \+formal_name: BG:DS05899.5 *F genefinder: *F genscan: 94 *F product: *F match_to: GB L42311 e-12 D (shn) *F match_to: GB U42403 e-10 D (sr) *F EST: *F P1: DS05899 *F direction: + *F SM_notes: Translation from Genscan prediction. *F ========================================================================== *F \+symbol: BG:DS05899.4 *F \+formal_name: BG:DS05899.4 *F genefinder: (14) *F genscan: 64 *F product: neuronal acetylcholine receptor, &agr;-7 chain precursor *F match_to: TR E1246208 e-102 H *F match_to: SP P36544 e-103 H *F match_to: SP P49582 e-104 M *F match_to: GB L37663 e-75 M *F match_to: SP P48180 e-99 W *F match_to: GB X83887 e-73 W *F match_to: SP P09478 e-62 D *F match_to: HL02253.5' D (Dros EST) *F EST: *F P1: DS05899 *F direction: + *F SM_notes: Translation from edited Genscan. Not DopR. Region 6.5kb *F upstream and 2 regions downstream with good homology to receptor but no *F start codon (stop codon just after nearest M). Removed last 2 exons of *F Genscan prediction (low prob.) and used for translation. *F =========================================================================== *F \+symbol: BG:DS01759.1 *F \+formal_name: BG:DS01759.1 *F genefinder: 77 *F genscan: 192 *F product: proline-rich region like mucin *F match_to: TR Q29071 e-11 O (pig) *F match_to: SP Q02817 e-9 H *F match_to: SP P37370 e-7 Y *F EST: *F P1: DS01759 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS01759.2 *F \+formal_name: BG:DS01759.2 *F genefinder: (14) *F genscan: 45 *F product: *F match_to: *F EST: HL03474.5' *F P1: DS01759 *F direction: - *F SM_notes: Translation from full-length HL03474. *F ========================================================================== *F \+symbol: BG:DS01523.1 *F \+formal_name: BG:DS01523.1 *F genefinder: 112 *F genscan: 230 *F product: *F match_to: GB W26043 e-18 H (Hum EST) *F EST: *F P1: DS01523 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS01523.2 *F \+formal_name: BG:DS01523.2 *F genefinder: 153 *F genscan: 510 *F product: repeated segment found in mucin *F match_to: SP Q02817 e-65 H *F match_to: TR Q61002 e-21 M *F match_to: TR O17893 e-51 W *F match_to: SP P08640 e-46 Y *F match_to: TR O23054 e-28 A *F match_to: GB D49534 e-12 D (cno) *F match_to: LD02410.5' *F e-6;LD17667.5';LD12158.5';LD34053.5';GM09485.5';LD033260.5';LD23668.5' D *F (Dros EST) *F EST: *F P1: DS01523 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: smi35A *F \+formal_name: BG:DS01523.3 *F genefinder: 48/33 *F genscan: 217 *F product: Dyrk2 kinase *F match_to: TR Q92630 e-81 H *F match_to: SP Q13627 e-48 H *F match_to: TR Q61214 e-48 M *F match_to: GB U58497 e-28 M *F match_to: TR Q20604 e-61 W *F match_to: SP P14680 e-33 Y *F EST: CK02015.5'; LD10161.5'; LD10161.3'; LD20817.5'; LD20817.3'; *F HL01379.5'; HL01379.3'; GM04027.5'; LD02884.5'; LD15788.5'; LD33744.5' *F P1: DS01523, DS01652, DS03792 *F direction: - *F P_element_allele: *F rff(2)k11509 sequenced *F smi35A1 sequenced by Anholt *F rff(2)k06901 sequenced *F SM_notes: Translation from full-length LD10161 on DS01523 edited *F (542-3970), DS01652 (426-541), DS03792 (1-425). k11509 maps to 82600 of *F DS03792 (first exon), *F k16716 maps to 53181, smi35A to 53174, and k06901 to 53160 of DS03792. *F =========================================================================== *F \+symbol: wb *F \+formal_name: BG:DS03792.1 *F synonym: l(2)34Fb *F name: wing blister *F UID: FBgn0004002 *F references: *F FBrf0046354 == Hilliker and Trusis-Coulter, 1987, Genetics 117: 233--244 *F cytology: 34F5--34F5 *F cytology_data: Left limit from inclusion within Df(2L)TE35B-10 (FBrf0091422) *F cytology_data: Right limit from inclusion within Df(2L)b78j (citation *F unavailable) *F genefinder: (14)/(16)/79(DS03792);68/150(DS01068) *F genscan: 337/(41)(DS03792);565(DS01068) *F product: laminin-&agr;2 subunit (merosin) *F match_to: SP P24043 e-128(DS01068) H laminin-&agr;2 *F match_to: GB X58531 e-112(DS03792) H laminin-&agr;1 *F match_to: SP P25391 e-112(DS03792)/e-125(DS01068) H laminin-&agr;1 *F match_to: SP Q60675 e-128(DS01068) M laminin-&agr;2 *F match_to: GB J04064 e-114(DS03792) M laminin-&agr;1 *F match_to: SP P19137 e-114(DS03792)/e-122(DS01068) M laminin-&agr;1 *F match_to: TR E1247268 e-122(DS03792) W laminin-like *F match_to: SP Q21313 e-95(DS01068) W laminin-like *F match_to: SP Q00174 e-104(DS01068) D laminin-&agr; *F EST: CK00390.5'; CK02229.5'; CK02229.3'; CK01592.3' *F P1: DS03792, DS01068 *F direction: + *F cDNA_sequence: AF135118.1 *F ems_allele: *F wb1 *F wb3 *F wb4 *F wb91g2 *F wbBMW22 *F wbBMW32 *F wbCH18 *F wbCH20 *F wbCH38 *F wbCH41 *F wbCH57 *F wbCH67 *F wbCH70 *F wbCR1 *F wbDM10 *F wbGM2 *F wbHG10 *F wbHG14 *F wbHG16 *F wbHG17 *F wbHG18 *F wbHG19 *F wbHG24 *F wbHG26 *F wbHG7 *F wbHG9 *F wbL412 *F wbOK14 *F wbPA43 *F wbSF19A *F wbSF20 *F wbSF25 *F tem_allele: *F wbSF11 *F P_element_allele: *F wb09437 sequenced Baumgartner *F wb10002 sequenced Baumgartner *F wbk06511 cluster: k06520 *F wbk08909 *F wbk14208 *F wbk05612 *F wbk00805 *F wbk13507 *F wbH155 sequenced Baumgartner *F rL918 sequenced *F n(2)k16716 sequenced *F PM_dysgenesis_allele: *F wbPI5 *F X_ray_allele: *F wbSF30 *F gamma_ray_allele: *F wbGR301 *F aberration_associated_allele: *F wb6r28 (T(2;3)6r28) *F wbDTD121 (In(2LR)DTD121) *F wbH68 (T(2;3)H68) *F misc_allele: *F wbIK4 *F phenotypic_class: behavioural. *F SM_notes: Translation from alignment of GenBank AF135118.1 on DS03792 (5'; *F 1-3644) and *F on DS01068 (3'; 3645-10817). n(2)k16716 (viable over deletion but *F phenotypically wb) maps to 53181 of DS03792, smi35A to 53174, *F rff(2)k06901 to 53160, l(2)09437 to 15219, n(2)rL918 to 14850. *F phenotypic_notes: escapers or weak alleles with wing blister phenotype. *F phenotypic_class: vital. *F JR_notes: product data from Baumgartner & Prokop. *F =========================================================================== *F symbol: ms(2)34Fe *F name: male sterile (2) 34Fe *F synonym: ms(2)34Fh *F UID: FBgn0002822 *F references: *F FBrf0051916 == Davis et al., 1990, Genetics 126: 105--119 *F cytology: 35A1--35A4 *F cytology_data: Left limit from complementation mapping against T(2;3)GT10 *F (citat *F ion unavailable) *F cytology_data: Right limit from inclusion within Df(2L)b80e3 (FBrf0091422) *F misc_allele: *F ms(2)34Fe1 *F phenotypic_class: male_sterile. *F JR_notes: 34Fe = 34Fh because these classes male sterile *F (b78j/do-1,b78j/el15,b78j/ms(2)34Fe1715,do-1/ms(2)34Fe1715,el15/ms(2)34Fe1715) *F and these classes male fertile *F (b85f1/do-1,b79b4/do-1,b85f1/el15,b79b4/el15,b85f1/ms(2)34Fe1715,b79b4/ms(2)34 *F Fe1715) since ms(2)34Fh *F was inferred from b78j/do-1 and b78j/el15 being ms. *F =========================================================================== *F symbol: l(2)34Fc *F name: lethal (2) 34Fc *F UID: FBgn0001968 *F cytology: 35A1--35A4 *F cytology_data: Left limit from inclusion within Df(2L)A217 (FBrf0038050) *F cytology_data: Right limit from inclusion within Df(2L)b80e3 (FBrf0038047) *F gamma_ray_allele: *F T(2;3)GT10 *F ems_allele: *F l(2)34Fc1 *F l(2)34Fc2 *F l(2)34Fc3 *F l(2)34Fc4 *F l(2)34Fc5 *F l(2)34Fc6 *F l(2)34Fc7 *F l(2)34Fc8 *F l(2)34Fc9 *F l(2)34Fc10 *F l(2)34FcOK9 *F phenotypic_class: vital. *F JR_notes: the synthetic deletion GT10 TE35BC-GV8 is hypomorphic for 34Fc, *F thus GT10 breaks in 34Fc. *F JR_notes: 3/37 excisions of k08712=Rab14 lethal; all lethal excisions *F 34Fd- or 35Aa-; therefore gene order is 34Fe, 34Fd, Rab14, 35Aa. *F =========================================================================== *F symbol: l(2)34Fd *F name: lethal (2) 34Fd *F UID: FBgn0001969 *F references: *F FBrf0051916 == Davis et al., 1990, Genetics 126: 105--119 *F cytology: 35A1--35A4 *F cytology_data: Left limit from inclusion within Df(2L)A217 (FBrf0038050) *F cytology_data: Right limit from inclusion within Df(2L)b80e3 (FBrf0091422) *F ems_allele: *F l(2)34Fd1 *F l(2)34Fd2 *F l(2)34Fd3 *F l(2)34Fd4 *F l(2)34Fd5 *F l(2)34Fd6 *F l(2)34Fd7 *F l(2)34Fd8 *F l(2)34Fd9 *F l(2)34FdCH35 *F phenotypic_class: vital. *F JR_notes: 3/37 excisions of k08712=Rab14 lethal; all lethal excisions *F 34Fd- or 35Aa-; therefore gene order is 34Fe, 34Fd, Rab14, 35Aa. *F =========================================================================== *F \+symbol: BG:DS03792.2 *F \+formal_name: BG:DS03792.2 *F genefinder: (20) *F genscan: 109 *F product: *F match_to: LD23113.5' e-7 *F EST: *F P1: DS03792 *F direction: + *F Nested_in: wb *F SM_notes: Translation from Genscan. Could be ms(2)34Fe. *F =========================================================================== *F \+symbol: BG:DS01068.1 *F \+formal_name: BG:DS01068.1 *F genefinder: 100 *F genscan: 397 *F product: *F match_to: *F EST: *F P1: DS01068 *F direction: + *F SM_notes: Could be ms(2)34Fe or l(2)34Fc. *F ========================================================================== *F \+symbol: BG:DS01068.11 *F \+formal_name: BG:DS01068.11 *F genefinder: (17)/172*(1 span) *F genscan: 487*(2 spans) *F product: *F match_to: GB AA313794 e-16 H (Homo EST) *F match_to: GB AA087241 e-14 M (Mus EST) *F match_to: SP P34664 e-10 W *F match_to: LD22379.5' e-22; LD34776.5' e-21; LD31155.5' e-21; LD06669.5' *F e-21; LD16573.5' e-21;LD16570.5' e-21;LD10017.5' e-21; LD21139.5' *F e-21;LD17209.5' e-21;LD17733.5' e-21 D (Dros EST) *F EST: GH05919.3' *F P1: DS01068 *F direction: + *F SM_notes: Translation from edited Genscan on 5' end and G05919.3' on 3' *F end. Could be ms(2)34Fe or l(2)34Fc. *F ========================================================================== *F \+symbol: BG:DS01068.2 *F \+formal_name: BG:DS01068.2 *F genefinder: 172*(6 spans) *F genscan: 487*(6 spans) *F product: *F match_to: TR O01893 e-101 W *F match_to: TR P73619 e-63 B (Synechoscystis sp.) *F match_to: GB R13905 e-18 H (Homo EST) *F match_to: GB T08003 e-18 H (Homo EST) *F match_to: GB AA451516 e-15 M (Mus EST) *F match_to: GB T76924 e-11 A (Arab EST) *F EST: *F P1: DS01068 *F direction: + *F SM_notes: Translation from edited Genscan. Could be ms(2)34Fe or l(2)34Fc. *F ========================================================================== *F \+symbol: BG:DS01068.10 *F \+formal_name: BG:DS01068.10 *F genefinder: 38 *F genscan: 30 *F product: similar to trypsin (serine protease) *F match_to: SP P15948 e-24 M *F match_to: SP P42278 e-31 D *F match_to: SP P54628 e-31 O (Drosophila erecta) *F EST: *F P1: DS01068 *F direction: - *F SM_notes: Translation from Genscan. Could be ms(2)34Fe or l(2)34Fc. *F ========================================================================== *F \+symbol: BG:DS01068.4 *F \+formal_name: BG:DS01068.4 *F genefinder: 41 *F genscan: 92 *F product: *F match_to: *F EST: LD33443.5'; LD22904.5' *F P1: DS01068 *F direction: - *F SM_notes: Translation from full-length LD33443. Could be ms(2)34Fe or *F l(2)34Fc. *F ========================================================================== *F \+symbol: BG:DS01068.5 *F \+formal_name: BG:DS01068.5 *F genefinder: 35 *F genscan: 382* (2 spans) *F product: *F match_to: *F EST: *F P1: DS01068 *F direction: - *F SM_notes: Translation from Genefinder. Could be ms(2)34Fe, l(2)34Fc, or *F l(2)34Fd. *F ========================================================================== *F \+symbol: BG:DS01068.6 *F \+formal_name: BG:DS01068.6 *F genefinder: 112 *F genscan: 382* (4 spans) *F product: *F match_to: TR Q19974 e-108 W (worm EST) *F match_to: SP P48234 e-82 Y *F match_to: GB Z72930 e-74 Y *F match_to: GB AA107580 e-41 M (Mus EST) *F match_to: GB X83354 e-25 M (Mus EST) *F match_to: GB AA314293 e-33 H (Hum EST) *F EST: LD09509.3'; LD09509.5'; GM05483.5'; GM06063.5'; GM08820.5'; *F LD21252.5'; LD32056.5' *F P1: DS01068 *F direction: - *F SM_notes: Translation from full-length LD09509. Best bet for 34Fd. *F JR_notes: Was thought to be broken by T(2;3)GT10 by in situ with LD09509, *F but *F this data disagrees with genetic data showing GT10 breaks in 34Fc. *F =========================================================================== *F \+symbol: Rab14 *F \+formal_name: BG:DS01068.7 *F full name: Rab-protein 14 *F synonym: ESTS:57H4T *F UID: FBgn0015791 *F cytology: 36A--36B *F references: *F FBrf0098074 == Ozaki, 1997.8.1 *F FBrf0093208 == Satoh et al., 1997, FEBS Lett. 404(1): 65--69 *F FBrf0077724 == Kohno et al., 1993, Zool. Sci., Tokyo 10: 119 *F FBrf0080163 == Kohno et al., 1994, Zool. Sci., Tokyo 11: 99 *F FBrf0086498 == Kono et al., 1995, Zool. Sci., Tokyo 12: 113 *F P_element_allele: *F n(2)k08712 sequenced *F genefinder: 39 *F genscan: 55 *F product: Rab14 *F match_to: SP P35287 e-79 M (rat) *F match_to: TR Q93874 e-73 W *F match_to: SP P20338 e-48 H *F match_to: GB AA214461 e-30 H (Hum EST) *F match_to: SP P38555 e-36 Y *F match_to: TR O23657 e-24 A *F EST: LD03340.3'; LD03340.5'; LD32887.5'; LD29476.5'; LD26806.5' *F P1: DS01068 *F direction: + *F cDNA_sequence: D84316 *F pr_sequence_SPTREMBL: O18336 *F phenotypic_class: not vital *F JR_notes: 3/37 excisions of k08712 lethal; all lethal excisions 34Fd- or *F 35Aa-, *F indicating that Rab14 is between 34Fd and 35Aa. Since 35Aa- deletion is *F rescued by a *F 5-kb GalNac (=35Aa) fragment (from Flores), and P in 5' end of Rab 14, *F Rab14 not vital. *F SM_notes: Translation from full-length LD03340 (longer than GenBank *F D84316). n(2)k08712 inserted at 39329 of DS01068; Rab14 at 39278-42449. *F =========================================================================== *F \+symbol: l(2)35Aa *F \+formal_name: BG:DS01068.8 *F UID: FBgn0001970 *F references: *F FBrf0051916 == Davis et al., 1990, Genetics 126: 105--119 *F cytology: 35A3--35A4 *F cytology_data: Left limit from inclusion within Df(2L)TE35B-7 (FBrf0051916) *F cytology_data: Right limit from inclusion within Df(2L)b80e3 (FBrf0091422) *F enzyme: polypeptide N-acetylgalactosaminyltransferase == EC 2.4.1.41 *F genefinder: 107 *F genscan: 263 *F match_to: TR Q10471 e-91 H *F match_to: GB X85019 e-83 H *F match_to: TR G3047191 e-91 W *F match_to: TR G3047193 e-91 W *F match_to: TR G3047195 e-91 W *F match_to: TR O08912 e-89 M *F EST: LD24449.5' *F P1: DS01068 *F direction: + *F ems_allele: *F l(2)35Aa1 *F l(2)35Aa2 *F l(2)35Aa3 *F l(2)35Aa4 *F l(2)35Aa5 *F l(2)35Aa6 *F l(2)35Aa7 *F phenotypic_class: vital. *F phenotypic_notes: early pupal lethal. *F SM_notes: Translation from full-length LD24449. *F JR_notes: Product & transformation rescue information from Flores (chez *F Engels). *F =========================================================================== *F \+symbol: spel1 *F \+formal_name: BG:DS01068.9 *F name: spellchecker1 *F UID: FBgn0015546 *F references: *F FBrf0078419 == rd1552 == Flores and Engels, 1995, A. Conf. Dros. Res. *F 36: 204A *F cytology: 35A1--35A1 *F cytology_data: Left limit from inclusion within Df(2L)A217 (FBrf0038050) *F cytology_data: Right limit from sequence databank entry U17893 *F genefinder: 83(DS01068);33(DS06238) *F genscan: 211(DS01068);33(DS06238) *F match_to: SP P43246 e-97(DS01068)/e-70(DS06238) H *F match_to: GB U04045 e-84(DS01068)/e-64(DS06238) H *F match_to: SP P43247 e-92(DS01068)/e-68(DS06238) M *F match_to: TR O24617 e-60(DS01068)/e-56(DS06238) A *F match_to: SP P25847 e-50(DS01068)/e-60(DS06238) Y *F EST: LD33650.5'; GM13404.5'; LD14009.5' *F P1: DS01068, DS06238 *F direction: + *F motifs: PS00486 == DNA mismatch repair proteins mutS family signature. *F homology: species == Escherichia coli; gene == mutS; ECOGENE: EG10625 *F homology: species == Mus; gene == Msh2; MGD: MRK-18634 *F homology: species == Homo sapiens; gene == MSH2; GDB: 203983; OMIM: 120435 *F cDNA_sequence:U17893; g675456 partial *F pr_sequence_SwissProt:P43248 *F phenotypic_class: non_vital. *F SM_notes: Translation from alignment of GenBank U17893 on DS01068 & DS06238. *F =========================================================================== *F \+symbol: ppk *F \+formal_name: BG:DS06238.1 *F name: pickpocket *F synonym: mdNaC1 *F UID: FBgn0020258 *F cDNA_sequence: Y16225; e1237841 *F cDNA_sequence: AF043263; g2811254 *F pr_sequence_TREMBL:O46104 *F pr_sequence_TREMBL:O44940 *F genefinder: (9)/28 *F genscan: 132 *F product: epithelial sodium channel. *F match_to: TR AF043264 e-61 D *F EST: *F P1: DS06238 *F direction: - *F SM_notes: Translation from alignment of GenBank on AF043263. *F ========================================================================== *F \+symbol: elB *F \+formal_name: BG:DS06238.3 *F name: elbow B *F UID: FBgn0004858 *F references: *F FBrf0051916 == Davis et al., 1990, Genetics 126: 105--119 *F cytology: 35A4--35A4 *F cytology_data: Left limit from inclusion within Df(2L)TE35B-14 (FBrf0092813) *F cytology_data: Right limit from inclusion within Df(2L)b83d29a (FBrf0051916) *F genefinder: 74 *F genscan: 199 *F product: noc-like Zinc-finger protein *F match_to: TR Q24423 e-59 D *F match_to: TR Q41805 e-7 O (Zea mays) *F EST: *F P1: DS06238 *F direction: - *F spontaneous_allele: *F see elA *F ems_allele: *F see elA *F gamma_ray_allele: *F see elA *F elB8 *F X_ray_allele: *F aberration_associated_allele: *F elB4 (T(Y;2)el4) *F elB6 (In(2LR)el6) *F elB7 (T(2;3)dpps19) *F elB9 (In(2L)el9) *F elB5 (T(Y;2)a15) *F P_element_allele: *F EP(2)2039 sequenced (elbow over a deletion) *F EP(2)0965 sequenced *F k07706 sequenced *F phenotypic_notes: reduced posterior compartment in wing; very similar, but *F complements, elA. *F SM_notes: Translation from Genscan. EP(2)2039 & sequence from Cohen at *F 45657 of DS06238; EP(2)0965 at *F 45828; k07706 at 45738. *F ========================================================================== *F \+symbol: BG:DS06238.4 *F \+formal_name: BG:DS06238.4 *F genefinder: 30 *F genscan: 63 *F product: larval cuticle protein *F match_to: GB M71249 e-11 D (Edg84A) *F match_to: SP P04929 e-33 O (Plasmodium lophurae) (His-rich, repeat segment) *F match_to: TR Q92504 e-11 H (His-rich, repeat segment) *F match_to: TR D1012195 e-11 H (His-rich, repeat segment) *F match_to: SP Q31125 e-11 M (His-rich, repeat segment) *F match_to: TR O35957 e-11 M *F match_to: TR O18272 e-9 W *F EST: *F P1: DS06238 *F direction: - *F SM_notes: Translation from Genscan. *F MA_notes: Best bet for pu, since matches Edg84A. *F ========================================================================== *F \+symbol: BG:DS08340.1 *F \+formal_name: BG:DS08340.1 *F genefinder: (9) *F genscan: (42) *F product: *F match_to: GB AA419695 e-8 M (Mus EST) *F match_to: GB AA778038 e-8 H (Hum EST) *F match_to: GH12842.5' e-19; LP06079.5' e-19; LP02268.5' e-19; GH06541.5' *F e-19; GH10679.5' e-19 D *F EST: *F P1: DS08340 *F direction: - *F SM_notes: Translation from Genscan. *F MA_notes: Best bet for elA. *F ========================================================================== *F symbol: pu *F name: pupal *F UID: FBgn0003161 *F references: *F FBrf0051916 == Davis et al., 1990, Genetics 126: 105--119 *F cytology: 35B1--35A4+ *F cytology_data: Left limit from complementation mapping against T(2;3)GT3 *F (citation unavailable) *F cytology_data: Right limit from inclusion within Df(2L)b83d29a (FBrf0051916) *F spontaneous_allele: *F pu1 *F ems_allele: *F pu2 *F pu3 *F pu4 *F pu5 *F pu6 *F pu7 *F phenotypic_notes: wings unexpanded. *F phenotypic_class: visible; non_vital. *F =========================================================================== *F symbol: elA *F name: elbow A *F UID: FBgn0004857 *F references: *F FBrf0051916 == Davis et al., 1990, Genetics 126: 105--119 *F cytology: 35B1--35B1 *F cytology_data: Left limit from inclusion within Df(2L)ARR1 (FBrf0051916) *F cytology_data: Right limit from inclusion within Df(2L)b81a1 (FBrf0038047) *F spontaneous_allele: *F elA1 (25-kb deletion) *F ems_allele: *F elA2 *F elA3 *F elA21 *F elA22 *F elA23 *F elA27 *F elA29 *F elA30 *F gamma_ray_allele: *F elA8 *F elA10 *F elA11 *F elA12 *F elA25 *F elA26 *F aberration_associated_allele: *F elA24 (T(2;3)el24) *F phenotypic_notes: reduced posterior compartment in wing; very similar, but *F complements, elB. *F phenotypic_class: visible; non_vital. *F =========================================================================== *F \+symbol: noc *F \+formal_name: BG:DS04641.1 *F name: no ocelli *F UID: FBgn0005771 *F references: *F FBrf0066938 == Cheah et al., 1994, Molec. Cell. Biol. 14: 1487--1499 *F FBrf0043115 == Chia et al., 1985, J. molec. Biol. 186: 689--706 *F FBrf0087599 == McNabb et al., 1996, Genetics 143(2): 897--911 *F cytology: 35B1+--35B1+ *F cytology_data: Left limit from inclusion within Df(2L)A178 (FBrf0038047) *F cytology_data: Right limit from inclusion within Df(2L)b81a1 (FBrf0038047) *F motifs: Zinc finger protein. *F genefinder: 88 *F genscan: 179 *F match_to: TR Q90237 e-11 O (Anolis pulchellus) *F match_to: SP P32323 e-7 Y *F EST: GM07914.5'; LD28078.5'; LD28077.5' *F P1: DS04641 *F direction: + *F cDNA_sequence:L14009; g457883 *F pr_sequence_TREMBL:Q24423 *F pr_sequence_PIR:A55929 *F ems_allele: *F noc18 *F noc19 *F noc22 *F noc23 *F noc35Ba-1 *F noc35Ba-2 *F noc35Ba-3 *F noc35Ba-4 *F nocSco-rv15 *F TE_insert_allele: *F nocTE35B (TE146) *F X_ray_allele: *F nocSco-rv27 *F gamma_ray_allele: *F noc14 *F noc15 *F noc21 *F noc6 *F aberration_associated_allele: *F noc2 (In(2L)noc2) *F noc4 (In(2LR)noc4) *F noc7 (In(2LR)noc7) *F nocGT8 (T(2;3)GT8) *F nocSco (Tp(2;2)Sco) *F nocSco-rv1 (In(2LR)Scorv1) *F nocSco-rv2 (In(2L)Scorv2) *F nocSco-rv5 (In(2L)Scorv5) *F nocSco-rv8 (In(2L)Scorv8) *F nocSco-rv9 (In(2LR)Scorv9) *F nocSco-rv11 (In(2L)Scorv11) *F nocSco-rv12 (Tp(2;2)Scorv12) *F nocSco-rv13 (T(2;3)Scorv13) *F nocSco-rv17 (In(2L)Scorv17) *F nocSco-rv21 (In(2L)Scorv21) *F nocSco-rv23 (Tp(2;1)Scorv23) *F nocSco-rv24 (In(2L)Scorv24) *F nocSco-rv26 (In(2L)Scorv26) *F TE146 derivatives *F P_element_allele: *F EP(2)2000 sequenced *F EP(2)2173 sequenced *F phenotypic_notes: visible phenotype is loss of ocelli; lethal may show *F failure of head involution; Sco is antimorphic allele. *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank L14009. *F =========================================================================== *F \+symbol: BG:DS04641.2 *F \+formal_name: BG:DS04641.2 *F genefinder: *F genscan: *F tRNAscan-SE: 57 *F product: tRNA-pro *F match_to: (GB M26848 e-6 M) *F EST: *F P1: DS04641 *F direction: + *F ========================================================================== *F \+symbol: tRNA:G3:35Ba *F \+formal_name: BG:DS04641.3 *F name: transfer RNA:gly3: 35Ba *F UID: FBgn0011862 *F references: *F FBrf0048836 == Meng et al., 1988, Nucleic Acids Res. 16: 7189 *F cytology: 35B1--35B2 *F genefinder: *F genscan: *F tRNAscan-SE: 72 *F product: tRNA-gly3 *F match_to: (GB Z14135 e-6 O (Bombyx mori)) *F match_to: (GB Z15047 e-6 O (Bombyx mori)) *F EST: *F P1: DS04641 *F direction: + *F na_sequence:X07778 *F =========================================================================== *F \+symbol: tRNA:G3:35Bb *F \+formal_name: BG:DS04641.4 *F name: transfer RNA:gly3: 35Bb *F UID: FBgn0011863 *F references: *F FBrf0048836 == Meng et al., 1988, Nucleic Acids Res. 16: 7189 *F cytology: 35B1--35B2 *F genefinder: *F genscan: *F tRNAscan-SE: 72 *F product: tRNA-gly3 *F match_to: GB M13661 e-9 H *F match_to: GB Z49226 e-12 O (Bombyx mori) *F match_to: TR O17337 e-8 W *F EST: *F P1: DS04641 *F direction: - *F na_sequence:X07778 *F =========================================================================== *F \+symbol: tRNA:G3:35Bc *F \+formal_name: BG:DS04641.5 *F name: transfer RNA:gly3: 35Bc *F UID: FBgn0011864 *F references: *F FBrf0048836 == Meng et al., 1988, Nucleic Acids Res. 16: 7189 *F cytology: 35B1--35B2 *F genefinder: *F genscan: *F tRNAscan-SE: 72 *F product: tRNA-gly3 *F match_to: GB M13661 e-9 H *F match_to: GB Z49226 e-12 O (Bombyx mori) *F EST: *F P1: DS04641 *F direction: - *F na_sequence:X07779 *F =========================================================================== *F \+symbol: tRNA:G3:35Bd *F \+formal_name: BG:DS04641.6 *F name: transfer RNA:gly3: 35Bd *F UID: FBgn0011865 *F references: *F FBrf0048836 == Meng et al., 1988, Nucleic Acids Res. 16: 7189 *F cytology: 35B1--35B2 *F genefinder: *F genscan: *F tRNAscan-SE: 72 *F product: tRNA-gly3 *F match_to: GB M13661 e-9 H *F match_to: GB Z49226 e-12 O (Bombyx mori) *F EST: *F P1: DS04641 *F direction: - *F na_sequence:X07779 *F =========================================================================== *F \+symbol: tRNA:G3:35Be *F \+formal_name: BG:DS04641.7 *F name: transfer RNA:gly3: 35Be *F UID: FBgn0011866 *F references: *F FBrf0048836 == Meng et al., 1988, Nucleic Acids Res. 16: 7189 *F cytology: 35B1--35B2 *F genefinder: *F genscan: *F tRNAscan-SE: 68 *F product: tRNA-gly3 *F match_to: GB M13661 e-9 H *F match_to: GB Z49226 e-12 O (Bombyx mori) *F EST: *F P1: DS04641 *F direction: - *F na_sequence:X07779 *F ========================================================================== *F \+symbol: BG:DS04641.8 *F \+formal_name: BG:DS04641.8 *F genefinder: 27 *F genscan: 94 *F product: *F match_to: *F EST: *F P1: DS04641 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS01486.1 *F \+formal_name: BG:DS01486.1 *F genefinder: (8) *F genscan: (44) *F product: ubiquitin--protein ligase *F match_to: SP Q16781 e-60 H *F match_to: SP P52490 e-50 Y *F match_to: GB AA271547 e-45 M (Mus EST) *F match_to: GB U62483 e-26 M *F match_to: SP P35129 e-31 W *F match_to: GB S70118 e-44 D *F match_to: GB L20126 e-44 D *F match_to: SP P35132 e-30 A *F match_to: LD24448.5' e-43; LD16328.c e-43; GM02759.5' e-43;LD14534.5'e-40; *F LD31794.5' e-35 *F EST: *F P1: DS01486 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS01486.2 *F \+formal_name: BG:DS01486.2 *F genefinder: *F genscan: *F tRNAscan-SE: 57 *F product: transfer RNA:pro *F match_to: GB X55888 e-9 O (chicken) *F EST: *F P1: DS01486 *F direction: + *F ========================================================================== *F \+symbol: BG:DS01486.3 *F \+formal_name: BG:DS01486.3 *F genefinder: *F genscan: *F tRNAscan-SE: 57 *F product: transfer RNA:pro *F match_to: GB X55888 e-10 O (chicken) *F EST: *F P1: DS01486 *F direction: - *F ========================================================================== *F \+symbol: BG:DS01486.4 *F \+formal_name: BG:DS01486.4 *F genefinder: *F genscan: *F tRNAscan-SE: 57 *F product: transfer RNA:pro *F match_to: GB X55888 e-9 O (chicken) *F EST: *F P1: DS01486 *F direction: + *F ========================================================================== *F \+symbol: osp *F \+formal_name: BG:DS01486.7 *F synonym: BG:DS01486.5 *F synonym: BG:DS07721.2 *F name: outspread *F UID: FBgn0003016 *F references: *F FBrf0087599 == McNabb et al., 1996, Genetics 143(2): 897--911 *F cytology: 35B3--35B3 *F cytology_data: Left limit from complementation mapping against T(2;3)GT7 *F (citation unavailable) *F cytology_data: Right limit from inclusion within Df(2L)A178 (FBrf0038047) *F genefinder: 94/34(DS01486); 9(DS07721) *F genscan: 431(DS01486); (30)* (2 spans)(DS07721) *F match_to: TR P97434 e-18 M (p116Rip) *F match_to: GB AA118415 e-10 M (Mus EST) *F match_to: SP P11055 e-10 H (myosin heavy chain) *F match_to: SP P13535 e-10 H (myosin heavy chain) *F match_to: TR Q20042 e-10 W *F match_to: TR Q18082 e-9 W (myosin heavy chain) *F match_to: SP P25386 e-8 Y *F EST: LD14119.5'; LD14119.3'; LD13781.5'; CK02594.5'; LD15891.5'; LD15891.3' *F P1: DS01486, (DS09219), DS07721 *F direction: - *F na_sequence:M14802; g552081 *F na_sequence:M17837; g903720 *F na_sequence:Z00030; g7559 *F pr_sequence_TREMBL:Q24231 *F pr_sequence_TREMBL:Q27421 *F ems_allele: *F osp1 *F osp16 *F osp19 *F osp24 *F osp25 *F osp27 *F osp4 *F osp6 *F osp7 *F osp76e *F osp77e *F osp8 *F ospCH63 *F diepoxyoctane_allele: *F osp80.1D *F P_element_allele: *F osprJ571 sequenced *F l(3)00865 sequenced *F k13218 sequenced *F gamma_ray_allele: *F osp104 *F osp201 *F osp29 *F osp35 *F osp40 *F osp83 *F aberration_associated_allele: *F osp22 (In(2L)osp22) *F osp3 (Tp(2;3)osp3) *F osp29 (Df(2L)osp29) *F osp59 (In(2L)osp59) *F osp90 (T(2;3)osp90) *F ospH47 (T(2;3)H47) *F ospMpe (T(2;3)Mpe) *F osppb3 (T(2;3)pb3) *F ospH86 (In(2L)dppH86) *F misc_allele: *F ospalw *F phenotypic_notes: wings outspread; not vital. *F phenotypic_class: visible; non_vital. *F MA_notes: includes BG:DS01486.6; Note that k13218 is not a phenotypic osp *F allele. *F SM_notes: Appears to be alternatively spliced: translation of osp-LD14119 *F from complete sequence LD14119 (not full-length on 5' end?), doesn't use *F penultimate *F exon of LD15891 but does use last exon (longer 3'). *F Translation of osp-3prime from full-length LD15891 on DS01486 (838-5324), *F osp-5prime on DS07721. *F =========================================================================== *F \+symbol: Adh *F \+formal_name: BG:DS01486.8 *F name: Alcohol dehydrogenase *F UID: FBgn0000055 *F cytology: 35B3--35B3 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35B-8 (FBrf0087599) *F cytology_data: Right limit from inclusion within Df(2L)A178 (FBrf0038047) *F enzyme: alcohol dehydrogenase == EC 1.1.1.1 *F genefinder: *F genscan: 132* (1 span) *F match_to: SP P15428 e-11 H *F match_to: TR Q61106 e-10 M *F EST: HL01670.5'; HL05782.5'; GH01616.5' *F P1: DS01486 *F direction: + *F motifs: PS00061 == Short-chain alcohol dehydrogenase family signature. *F na_sequence:J01066 *F na_sequence:M14802; g156803 *F na_sequence:M17827; g156860 *F na_sequence:M17828; g156862 *F na_sequence:M17830; g156866 *F na_sequence:M17831; g156868 *F na_sequence:M17832; g156870 *F na_sequence:M17833; g156872 *F na_sequence:M17834; g156874 *F na_sequence:M17835; g156876 *F na_sequence:M17836; g156878 *F na_sequence:M17837; g156880 *F na_sequence:M17845; g156906 *F na_sequence:M19547; g156864 *F na_sequence:M22210; g156839 *F na_sequence:M29635 *F na_sequence:M29636; e124826 *F na_sequence:M36580; g156805 *F na_sequence:M57239; g156800 *F na_sequence:M71284 *F na_sequence:M73256 *F na_sequence:U20765; g684944 *F na_sequence:U35852 *F na_sequence:X04454 *F na_sequence:X15607 *F na_sequence:X60791; g8152 *F na_sequence:X60792; g8441 *F na_sequence:X60793; g8282 *F na_sequence:X66233 *F na_sequence:X78384; e315259 *F na_sequence:X78385 *F na_sequence:X78386 *F na_sequence:X78387 *F na_sequence:X78402 *F na_sequence:X78916 *F na_sequence:X78917 *F na_sequence:X98338; e248823 *F na_sequence:Z00030; g599727 *F pr_sequence_SwissProt:P00334 *F pr_sequence_TREMBL:Q27332 *F pr_sequence_TREMBL:Q27579 *F pr_sequence_TREMBL:Q27596 *F pr_sequence_PIR:A37092 *F pr_sequence_PIR:B93309 *F pr_sequence_PIR:A93713 *F pr_sequence_PIR:A93875 *F pr_sequence_PIR:A93309 *F pr_sequence_PIR:A93873 *F pr_sequence_PIR:S17083 *F pr_sequence_PIR:S17084 *F phenotypic_class: visible; non_vital. *F Nested_in: osp *F SM_notes: Translation from alignment of trEMBL Q27332. *F =========================================================================== *F \+symbol: Adhr *F \+formal_name: BG:DS01486.9 *F name: Adh-related *F UID: FBgn0000056 *F references: *F Brogna, EMBO J. 16: 2023-2031 *F cytology: 35B3--35B3 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35B-8 (FBrf0091422) *F cytology_data: Right limit from inclusion within Df(2L)A178 (FBrf0091422) *F genefinder: (15) *F genscan: 132* (2 spans) *F product: alcohol-dehydrogenase \like *F match_to: SP P15428 e-11 H *F match_to: TR Q61106 e-10 M *F EST: GM04306.5' *F P1: DS01486 *F direction: + *F na_sequence:M14802; g765036 *F na_sequence:Z00030 *F na_sequence:X78384; g483719 *F na_sequence:X98338; e248898 *F pr_sequence_TREMBL:Q24230 *F pr_sequence_TREMBL:Q24232 *F phenotypic_notes: complete deletion associated with malformation of gastric *F caecea. *F phenotypic_class: visible; non_vital. *F Nested_in: osp *F SM_notes: Translation from alignment of SwissProt P91615. *F ========================================================================== *F \+symbol: BG:DS09219.1 *F \+formal_name: BG:DS09219.1 *F genefinder: *F genscan: (41)(DS09219)/(34)(DS07721) *F product: *F match_to: *F EST: *F P1: DS09219, DS07721 *F direction: - *F Nested_in: osp *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS07721.1 *F \+formal_name: BG:DS07721.1 *F genefinder: *F genscan: *F product: *F match_to: *F EST: CK02594.5'; CK02594.3' *F P1: DS07721 *F direction: + *F Nested_in: osp *F SM_notes: Translation from full-length CK02594. *F =========================================================================== *F \+symbol: BG:DS07721.3 *F \+formal_name: BG:DS07721.3 *F genefinder: 21 *F genscan: 47/(44) *F product: *F match_to: LP02495.5' e-8; LP04185.5' e-8; LP03565.5' e-8; LP03436.5' e-8 *F EST: *F P1: DS07721 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS07721.6 *F \+formal_name: BG:DS07721.6 *F genefinder: *F genscan: 271 *F match_to: *F EST: *F P1: DS07721 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS00810.1 *F \+formal_name: BG:DS00810.1 *F genefinder: 25 *F genscan: 97 *F product: *F match_to: GB AA313794 e-14 H (Homo EST) *F match_to: SP P34664 e-14 W *F match_to: GB AA087241 e-10 M (Mus EST) *F match_to: LD20357.5' e-13;LD22379.5' e-12;LD34776.5' e-12;LD31155.5' *F e-12;LD16573.5' e-12;LD16570.5' e-12;LD06669.5' e-12;LD21139.5' *F e-12;LD28856.5' e-12;LD15550.5' e-12 *F EST: *F P1: DS00810 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS00810.2 *F \+formal_name: BG:DS00810.2 *F genefinder: (19) *F genscan: *F product: ribosomal protein 15a *F match_to: GB Z21673 e-47 D *F match_to: LD11847.3' e-25; GM02304.5' e-27; GM04832.5' e-25; GM06369.5' *F e-54; GM13983.5' e-48; HL07857.5' e-43; LD03678.5' e-25; LD04294.5' e-21; *F LD07181.5' e-50; LD14972.5' e-25; LD33594.5' e-48; GH04971.3' e-45 *F EST: LP03565.5'; LP04185.5'; LP02495.5'; LP04636.3'; LP06216.3'; LP03436.5' *F P1: DS00810 *F direction: - *F SM_notes: Translation from full-length LP03565. *F ========================================================================== *F \+symbol: BG:DS00810.3 *F \+formal_name: BG:DS00810.3 *F genefinder: (16) *F genscan: (14) *F product: *F match_to: SP Q25055 e-10 O *F match_to: SP P17816 e-12 O *F match_to: TR O02049 e-9 W *F EST: GH13704.5'; GH07880.5'; LP02868.5'; LP03582.5' *F P1: DS00810 *F direction: + *F SM_notes: Translation from complete sequence GH13704 at 5' (not full-length *F clone?) and GH07880.5' EST at 3'.. *F =========================================================================== *F \+symbol: BG:DS06874.1 *F \+formal_name: BG:DS06874.1 *F genefinder: 27 *F genscan: 59 *F product: *F match_to: *F EST: *F P1: DS06874 *F direction: + *F SM_notes: Translation from Genefinder. *F ========================================================================== *F \+symbol: BG:DS06874.2 *F \+formal_name: BG:DS06874.2 *F genefinder: (19) *F genscan: 111 *F product: *F match_to: SP P97834 e-58 (rat) *F match_to: GB X87885 e-57 (rat) *F match_to: SP Q13098 e-56 H *F match_to: SP P45432 e-37 A *F match_to: GB W65922 e-13 M (Mus EST) *F EST: *F P1: DS06874 *F direction: + *F SM_notes: Translation from Genscan. *F MA_notes: Fusca; GPS1 homolog see MCB 16: 6698-6706 *F =========================================================================== *F \+symbol: BG:DS06874.3 *F \+formal_name: BG:DS06874.3 *F genefinder: 44 *F genscan: 127 *F product: MSP1 homolog (TAT-binding) *F match_to: SP P54815 e-62 W *F match_to: YPD 1323004 e-62 Y *F match_to: GB X68055 e-56 Y *F match_to: GB X81069 e-58 Y *F match_to: TR G3193292 e-50 A *F match_to: GB AA028745 e-36 M (Mus EST) *F match_to: GB AA268776 e-36 M (Mus EST) *F match_to: TR G2984586 e-33 H *F match_to: TR G3283072 e-33 H *F match_to: SP P91638 e-33 D *F match_to: GH13949.5' e-58; LP05581.5' e-39; GH08677.5' e-39; LD20618.5' *F e-22;GM07738.5' e-12;GM05721.5' e-15;GM04824.5' e-12;CK00306.5' e-28 *F EST: *F P1: DS06874 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS06874.4 *F \+formal_name: BG:DS06874.4 *F genefinder: (16) *F genscan: 103*(2 spans) *F product: serine protease *F match_to: SP P40313 e-13 H *F match_to: TR D1029835 (AB015206) e-11 M *F match_to: SP P05049 e-10 D *F EST: *F P1: DS06874 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS06874.6 *F \+formal_name: BG:DS06874.6 *F genefinder: *F genscan: (35) *F product: serine protease *F match_to: TR D1029835 (AB015206) e-8 M *F match_to: SP P40313 e-13 H *F EST: *F P1: DS06874 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS06874.7 *F \+formal_name: BG:DS06874.7 *F genefinder: *F genscan: 119 *F product: *F match_to: *F EST: *F P1: DS06874 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS03431.1 *F \+formal_name: BG:DS03431.1 *F genefinder: 55 *F genscan: 111 *F product: proline transporter *F match_to: TR G3252836 e-115 O (tobacco hornworm) *F match_to: SP P51905 e-74 D *F match_to: SP Q99884 e-69 H *F match_to: TR Q99884 e-69 H *F match_to: SP P31659 e-63 M *F match_to: TR O17372 e-54 W *F match_to: LD18557.5' e-31 *F EST: *F P1: DS03431 *F direction: - *F SM_notes: Translation from Genscan. ether resistant P-element insert; may *F or may-not be this. *F =========================================================================== *F \+symbol: Mst35Ba *F \+formal_name: BG:DS03431.2 *F name: Male-specific-transcript-35Ba *F references: *F FBrf0058368 == Russell and Kaiser, 1993, Europ. Dros. Res. Conf. 13: n.p. *F cytology: 35B1--35B10 *F genefinder: *F genscan: *F product: protamine \like *F match_to: SP P17502 e-7 O (boll weevil) *F EST: *F P1: DS03431 *F direction: - *F cDNA_sequence:Z46790; g608696 *F genomic_sequence:Z46783; g608700 *F pr_sequence_TREMBL:Q24401 *F pr_sequence_TREMBL:Q24405 *F pr_sequence_PIR:S52155 *F pr_sequence_PIR:S52157 *F SM_notes: Translation from GenBank Z46790. *F =========================================================================== *F \+symbol: Mst35Bb *F \+formal_name: BG:DS03431.3 *F name: Male-specific-transcript-35Bb *F references: *F FBrf0058368 == Russell and Kaiser, 1993, Europ. Dros. Res. Conf. 13: n.p. *F cytology: 35B1--35B10 *F genefinder: (11) *F genscan: *F product: protamine \like *F match_to: *F EST: *F P1: DS03431 *F direction: - *F cDNA_sequence:Z46784; g608698 *F genomic_sequence:Z46785; g608702 *F pr_sequence_TREMBL:Q24402 *F pr_sequence_TREMBL:Q24406 *F pr_sequence_PIR:S52156 *F pr_sequence_PIR:S52158 *F SM_notes: Translation from GenBank Z46784. *F =========================================================================== *F symbol: ms(2)35Bi *F JR_notes: Inferred from deletion o/laps. *F =========================================================================== *F \+symbol: BG:DS03144.1 *F \+formal_name: BG:DS03144.1 *F genefinder: 49/(11) *F genscan: 123 *F product: *F match_to: *F EST: *F P1: DS03144 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS03323.1 *F \+formal_name: BG:DS03323.1 *F genefinder: 102(DS03323) *F genscan: 133(DS03144);405(DS03323) *F product: strawberry-notch-like *F match_to: GB U95760 e-197 D (sno) *F match_to: TR O01737 e-241 W *F match_to: TR G3152595 e-176 A *F match_to: GB AA413592 e-51 M (Mus EST) *F match_to: GB W25975 e-31 H (Hum EST) *F match_to: HL01650.5' e-24 *F EST: *F P1: DS03144, DS03323 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS01219.3 *F \+formal_name: BG:DS01219.3 *F genefinder: (10) *F genscan: 99 *F product: *F match_to: *F EST: *F P1: DS01219 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F symbol: l(2)35Bb *F name: lethal (2) 35Bb *F UID: FBgn0001972 *F references: *F FBrf0087599 == McNabb et al., 1996, Genetics 143(2): 897--911 *F cytology: 35B4--35B4 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-34 (FBrf0091422) *F cytology_data: Right limit from inclusion within Df(2L)b84a3 (FBrf0055544) *F spontaneous_allele: *F l(2)35Bb6 *F ems_allele: *F l(2)35Bb1 *F l(2)35Bb2 *F l(2)35Bb3 *F l(2)35Bb4 *F l(2)35Bb5 *F l(2)35Bb7 *F l(2)35Bb8 *F l(2)35Bb9 *F neutron_induced_allele: *F l(2)35Bb10 *F P_element_induced_allele: *F l(2)35Bbk11524 sequenced(DS00929) *F phenotypic_class: vital. *F SM_notes: k11524p inserted at 5037 of DS00929, in ORF of BG:DS00929.16, but *F BG:DS01219.1 ESTs from 1533-1359 of DS00929. *F =========================================================================== *F symbol: l(2)35Bf *F name: lethal (2) 35Bf *F UID: FBgn0001976 *F references: *F cytology: 35B4--35B4 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-4 (FBrf0038053) *F cytology_data: Right limit from inclusion within Df(2L)b84a3 (FBrf0055544) *F ems_allele: *F l(2)35Bf1 *F l(2)35Bf2 *F l(2)35Bf3 *F l(2)35Bf4 *F l(2)35Bf5 *F l(2)35Bf6 *F l(2)35Bf7 *F l(2)35Bf8 *F l(2)35BfQL53 *F phenotypic_class: vital. *F JR_notes: Excision of k08808=l(2)35Bc indicates gene order is 35Bb, 35Bf, *F 35Be, 35Bc, 35Bd. *F ========================================================================== *F symbol: l(2)35Be *F name: lethal (2) 35Be *F UID: FBgn0001975 *F references: *F cytology: 35B4--35B4 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-4 (FBrf0038053) *F cytology_data: Right limit from inclusion within Df(2L)b84a3 (FBrf0055544) *F ems_allele: *F l(2)35Be1 *F l(2)35Be2 *F l(2)35Be3 *F l(2)35Be4 *F l(2)35Be5 *F l(2)35Be6 *F l(2)35Be7 *F phenotypic_class: vital. *F JR_notes: Excision of k08808=l(2)35Bc indicates gene order is 35Bb, 35Bf, *F 35Be, 35Bc, 35Bd. *F =========================================================================== *F symbol: l(2)35Bc *F name: lethal (2) 35Bc *F UID: FBgn0001973 *F references: *F cytology: 35B4--35B4 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-4 (FBrf0038053) *F cytology_data: Right limit from inclusion within Df(2L)b84a3 (FBrf0055544) *F ems_allele: *F l(2)35Bc1 *F l(2)35Bc2 *F l(2)35Bc3 *F l(2)35Bc4 *F l(2)35Bc5 *F l(2)35Bc6 *F l(2)35Bc7 *F l(2)35Bc8 *F P_element_allele: *F l(2)35Bck08808 sequenced *F phenotypic_class: vital. *F SM_notes: yoyo is in intron of l(2)35Bb; P sequences map to yoyo LTRs *F (therefore nested in 35Bb).. *F The following P-elements match the sequence of the yoyo LTR but some may be *F from other sites: *F l(3)06264, l(2)01092, EP(x)1031, l(2)rH280, l(2)k00611, EP(2)0533, *F EP(3)0396, EP(x)1102, l(3)j2D5, l(3)rL061 *F JR_notes: Of 84 viable and 38 lethal excisions of k08808, 3 are deletions: *F 1 mutant for 35Be and 35Bc, 2 mutant for 35Bc and 35Bd. *F This suggests order is 35Bb, 35Bf, 35Be, 35Bc, 35Bd. PZ06264 is a 2nd site *F insertion. *F =========================================================================== *F \+symbol: BG:DS01219.1 *F \+formal_name: BG:DS01219.1 *F genefinder: 40/9 *F genscan: 53(2 spans)/37(1 span)(DS01219) *F product: *F match_to: SP P34298 e-11 W *F EST: LD16050.5'; LD16050.3'; LD13118.5'; LD13118.3'; LD14488.5'; *F LD17401.5'; LD27791.5' *F P1: DS01219, DS00929 *F direction: - *F SM_notes: Translation from full-length LD16050 3' on DS01219 (166-1993), *F 5' on DS00929 (1-165). k11524p (l(2)35Bb) inserted at 5037 of DS00929, *F ESTs from *F 1533-1359 of DS00929, so could be l(2)35Bb. *F =========================================================================== *F \+symbol: BG:DS00929.16 *F \+formal_name: BG:DS01219.16 *F genefinder: *F genscan: 71 *F product: *F match_to: *F EST: *F P1: DS00929 *F direction: + *F SM_notes: Translation from 5' edited Genscan (missing 5' end?). Good bet *F for l(2)35Bb because P element k11524p maps to 5037 of *F DS00929 and one ORF of genscan prediction is 4992-5160. *F JR_notes: Could also be l(2)35Bf. *F =========================================================================== *F \+symbol: BG:DS00929.1 *F \+formal_name: BG:DS00929.1 *F genefinder: 21 *F genscan: 49 *F product: *F match_to: GB AA221255 e-32 M (Mus EST) *F match_to: GB AA245336 e-32 M (Mus EST) *F match_to: GB AA166258 e-32 M (Mus EST) *F match_to: GB AA25934 e-27 H (Hum EST) *F match_to: GB AA004508 e-10 H (Hum EST) *F EST: *F P1: DS00929 *F direction: + *F SM_notes: Translation from Genscan. Could be l(2)35Bf or l(2)35Be. *F ========================================================================== *F \+symbol: BG:DS00929.2 *F \+formal_name: BG:DS00929.2 *F genefinder: 43 *F genscan: 75 *F product: ankyrin-like *F match_to: GB AA200445 e-17 M (Mus EST) *F match_to: GB AA200439 e-17 M (Mus EST) *F match_to: TR Q19822 e-14 W *F match_to: SP P16157 e-10 H *F match_to: TR Q24241 e-7 D *F EST: LD17234.5'; LD17234.3' *F P1: DS00929 *F direction: - *F SM_notes: Translation from full-length LD17234. Could be l(2)35Bf, *F l(2)35Be, or l(2)35Bc. *F =========================================================================== *F \+symbol: BG:DS00929.3 *F \+formal_name: BG:DS00929.3 *F genefinder: 44 *F genscan: 70 *F product: TATA-binding protein associated phosphoprotein *F match_to: SP Q01658 e-33 H *F match_to: GB M97388 e-31 H *F match_to: GB AA413894 e-31 M (Mus EST) *F match_to: TR E1246915 e-19 W *F match_to: SP P49592 e-17 A *F match_to: TR Q92317 e-10 Y *F match_to: YPD 1666487 e-16 Y *F EST: *F P1: DS00929 *F direction: + *F SM_notes: Translation from Genscan. Could be l(2)35Be or l(2)35Bc. *F ========================================================================== *F \+symbol: BG:DS00929.4 *F \+formal_name: BG:DS00929.4 *F genefinder: 29 *F genscan: 62 *F product: *F match_to: SP P53215 e-56 Y *F match_to: GB Z72809 e-55 Y *F match_to: GB T04261 e-29 A *F match_to: GB AA450700 e-23 M (Mus EST) *F match_to: GB W77781 e-7 H (Hum EST) *F EST: *F P1: DS00929 *F direction: - *F SM_notes: Translation from Genscan. Could be l(2)35Bc. *F =========================================================================== *F \+symbol: l(2)35Bd *F \+formal_name: BG:DS00929.5 *F name: lethal (2) 35Bd *F UID: FBgn0001974 *F references: *F cytology: 35B4--35B4 *F cytology_data: Left limit from inclusion within Df(2L)AntpCtxrv1 *F (FBrf0092813) *F cytology_data: Right limit from inclusion within Df(2L)b84a3 (FBrf0055544) *F genefinder: 49 *F genscan: 684*(2 spans) *F product: mRNA cap methyltransferase *F match_to: TR D1024575 e-72 H *F match_to: SP P32783 e-24 Y *F match_to: GB L12000 e-22 Y *F match_to: GB Z81038 e-11 W *F match_to: GB T21843 e-8 A *F EST: LD09819.3'; LD09819.5'; LD27566.5'; LD02165.5'; LD07429.5'; *F LD10977.5'; LD13245.5'; LD13885.5'; LD19803.5'; LD21076.5'; LD21952.5'; *F LD22017.5'; LD26541.5'; LD26968.5'; LD27714.5' *F P1: DS00929 *F direction: + *F ems_allele: *F l(2)35Bd1 *F l(2)35Bd2 *F l(2)35Bd3 *F l(2)35Bd4 *F l(2)35Bd5 *F l(2)35Bd6 *F P_element_allele: *F l(2)35Bd10408 sequenced *F aberration_associated_allele: *F l(2)35BdCtx (Tp(3;2)AntpCtx) *F phenotypic_class: vital. *F SM_notes: Translation from full-length LD09819. l(2)35Bd 17448-19229 *F (ESTs), l(2)10408 at ~17507 (plasmid rescue). *F =========================================================================== *F \+symbol: BG:DS00929.6 *F \+formal_name: BG:DS00929.6 *F genefinder: 8/31 *F genscan: 684*(16 spans) *F product: GABA receptor *F match_to: TR O08621 e-139 O (rat) *F match_to: GB Y10370 e-135 O (rat) *F match_to: GB W48985 e-30 M (Mus EST) *F match_to: GB AA036134 e-12 M (Mus EST) *F match_to: GB X90543 e-18 H (Hum EST) *F match_to: TR Q23442 e-15 W *F match_to: GB U58748 e-9 W *F EST: *F P1: DS00929 *F direction: + *F SM_notes: Translation from 5' and 3' edited Genscan (missing 5' end?). *F ========================================================================== *F \+symbol: BG:DS00929.7 *F \+formal_name: BG:DS00929.7 *F genefinder: *F genscan: *F product: ficolin *F match_to: SP P21520 e-23 D *F match_to: TR O18546 e-30 O (bloodfluke planorb) *F match_to: TR D1026054 e-28 M *F match_to: TR Q28763 e-27 H *F match_to: TR O17043 e-7 W *F EST: *F P1: DS00929 *F direction: - *F SM_notes: Translation from 3' edited BLASTX homology to GenBank P21520. *F ========================================================================== *F \+symbol: BG:DS00929.8 *F \+formal_name: BG:DS00929.8 *F genefinder: 66 *F genscan: 684*(4 spans) *F product: yellow-like *F match_to: SP P09957 e-74 D *F match_to: TR O18330 e-21 O (honey bee) *F EST: *F P1: DS00929 *F direction: + *F SM_notes: Translation from 5' edited Genscan. *F ========================================================================== *F \+symbol: l(2)35Bg *F \+formal_name: BG:DS00929.9 *F synonym: anon-35Ba *F name: lethal (2) 35Bg *F UID: FBgn0001977 *F UID: FBgn0015867 *F references: *F FBrf0055545 == Schweisguth and Posakony, 1992, Cell 69: 1199--1212 *F cytology: 35B10--35B10 *F cytology_data: Left limit from non-inclusion within Df(2L)b80k (FBrf0092813) *F cytology_data: Right limit from inclusion within Df(2L)ARR1 (FBrf0038053) *F ems_allele: *F l(2)35Bg1 *F l(2)35Bg2 *F PM_dysgenesis_allele: *F l(2)35BgPI2 *F P_element_allele: *F k10011 sequenced (semilethal) *F phenotypic_class: vital. *F genefinder: 41 *F genscan: 47 *F product: *F match_to: TR G3252826 e-14 H *F match_to: YPD YKR071C e-9 Y *F match_to: GB AA289143 e-7 M (Mus EST) *F match_to: SP P41847 e-7 W *F EST: GM08660.5'; GM08660.3';GM10279.5'; LD23249.5'; LD32407.5' *F P1: DS00929 *F direction: - *F SM_notes: Translation from full-length LD32407. cDNA overlaps BG:DS00929.8 *F but on opposite strand--does this mean BG:DS00929.8 not real gene? *F l(2)35Bg 46305-43777(ESTs), l(2)k10011 inserted 46297 DS00929. *F ========================================================================== *F \+symbol: Su(H) *F \+formal_name: BG:DS00929.10 *F synonym: l(2)35Bh *F name: Suppressor of Hairless *F UID: FBgn0004837 *F references: *F FBrf0054340 == Furukawa et al., 1991, J. Biol. Chem. 266: 23334--23340 *F FBrf0055544 == Furukawa et al., 1992, Cell 69: 1191--1197 *F FBrf0079374 == Schweisguth et al., 1994, Dev. Biol. 166(2): 812--814 *F FBrf0079375 == Schweisguth, 1995, Development 121(6): 1875--1884 *F FBrf0068637 == Posakony, 1994, Cell 76(3): 415--418 *F FBrf0074389 == Schweisguth and Posakony, 1994, Development 120(6): *F 1433--1441 *F FBrf0055545 == Schweisguth and Posakony, 1992, Cell 69: 1199--1212 *F FBrf0084115 == Lecourtois and Schweisguth, 1995, Genes Dev. 9(21): *F 2598--2608 *F cytology: 35B10--35B10 *F cytology_data: Left limit from non-inclusion within Df(2L)b80k (FBrf0092813) *F cytology_data: Right limit from inclusion within Df(2L)ARR1 (FBrf0038053) *F product: DNA-binding-protein *F genefinder: 80 *F genscan: 188 *F match_to: GB M81871 e-175 M *F match_to: GB X17459 e-175 M *F match_to: SP P31266 e-176 M *F match_to: SP Q06330 e-158 H *F match_to: GB L34543 e-157 H *F match_to: GB L34544 e-157 H *F match_to: TR Q27427 e-107 W *F match_to: GB U49794 e-98 W *F match_to: YPD 825569 e-10 Y *F EST: LD17227.5'; GM10826.5'; LD22806.5'; LD24729.5' *F P1: DS00929 *F direction: + *F homology: species == Mus; gene == Igkrsbp; MGD: MRK-11327 *F homology: species == Homo sapiens; gene == IGKJRB; GDB: 138293; OMIM: *F 147183 MEDLINE: 94010923 *F homology: species == Homo sapiens; gene == IMAGE: 32996; EMBL: R19314; DRES: 55 *F cDNA_sequence:X58393; g10957 *F cDNA_sequence:M94383; g158515 *F pr_sequence_SwissProt:P28159 *F pr_sequence_PIR:A41585 *F pr_sequence_PIR:A42770 *F spontaneous_allele: *F Su(H)1 *F Su(H)2 *F ems_allele: *F Su(H)3 *F Su(H)4 *F Su(H)5 *F Su(H)6 *F Su(H)7 *F Su(H)9 *F Su(H)AM1 *F Su(H)10 *F Su(H)11 *F Su(H)16 *F Su(H)IB115 *F Su(H)O5 *F Su(H)T4 *F Su(H)eBC11 *F Su(H)sA21 *F Su(H)sK2 *F Su(H)sO10 *F Su(H)E86 *F tem_allele: *F Su(H)8 *F PM_dysgenesis_allele: *F Su(H)12 *F Su(H)13 *F Su(H)14 *F Su(H)15 *F P{X6}Su(H)M28=Su(H)1356 *F P_element_allele: *F k07904 sequenced *F P_element_derivative_allele: *F Su(H)rv1 *F misc_allele: *F Su(H)E(H) *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank M94383. *F =========================================================================== *F \+symbol: ck *F \+formal_name: BG:DS00929.11 *F name: crinkled *F synonym: Mhc35BC; UID: FBgn0015260; *F synonym: anon-35Bb *F UID: FBgn0000317 *F references: *F FBrf0083719 == Agrawal et al., 1995, Dev. Biol. 172(1): 218--229 *F FBrf0054380 == Chen et al., 1991, J. Cell Biol. 115(3/2): 330a *F FBrf0089920 == Wright and Jackson, 1996, Trends Genet. 12(6): 206--209 *F FBrf0055545 == Schweisguth and Posakony, 1992, Cell 69: 1199--1212 *F cytology: 35B10+--35B10+ *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-3 (FBrf0038047) *F cytology_data: Right limit from inclusion within Df(2L)TE35BC-7 (FBrf0055544) *F product: myosin VIIA *F genefinder: 179 *F genscan: 630 *F match_to: GB U39226 0 H *F match_to: GB U81453 0 M *F match_to: TR P91443 0 W *F match_to: GB U80848 0 W *F match_to: TR G3142302 e-128 A *F match_to: SP P19524 e-112 Y *F match_to: GB Z75234 e-112 Y *F EST: LD10736.3'; LD10736.5'; LD14917.5'; LD14874.5' *F P1: DS00929 *F direction: - *F spontaneous_allele: *F ck1 note that the existing ck1 allele is l(2)35Fb *F ems_allele: *F ck2 *F ck3 *F ck4 *F ck5 *F ck6 *F ck7 *F ck8 *F ck9 *F ck10 *F ck11 *F ck12 *F ck13 *F ck14 *F ck15 *F ck16 *F ck17 *F ckA11 *F ckGJ2 *F P_element_allele: *F ck07130 sequenced *F EP2051 sequenced *F aberration_associated_allele: *F ckH67 (T(2;3)H67) *F misc_allele: *F ckIK3 *F TE_insert_allele: *F ckTE35BC (TE36) *F TE36 derivatives *F phenotypic_notes: cell polarity gene; affects denticle belts, microchaetae etc. *F phenotypic_class: visible; non_vital. *F SM_notes: Translation from alignment of cDNA from D. Kiehart (unfinished). *F PZ07130 at nt 65322, first exon of Kiehart's cDNA 65294-65143. *F EP2051 at 62404 of DS00929. *F =========================================================================== *F \+symbol: TfIIS *F \+formal_name: BG:DS00929.12 *F name: RNA polymerase II elongation factor *F synonym: l(2)35Cf *F synonym: DmS2 *F UID: FBgn0010422 *F UID: FBgn0001982 (l(2)35Cf) *F product: RNA-polymerase-II-elongation-factor *F motifs: PS00466 == TFIIS zinc ribbon domain signature. *F genefinder: 69 *F genscan: 128 *F match_to: TR E1309841 e-62 M *F match_to: TR Q15560 e-61 H *F match_to: SP P52652 e-45 W *F match_to: YPD 1322528 e-28 Y *F EST: LD32510.5'; LD34766.5'; LD33310.5'; LD15545.5'; GM02906.5'; *F GM03078.5'; GM04006.5'; GM09514.5'; LD18436.5' *F P1: DS00929 *F direction: - *F genomic_sequence:L26091; g416355 *F cDNA_sequence:X53670; g7921 *F pr_sequence_PIR:S12106 *F pr_sequence_PIR:S55899 *F pr_sequence_SwissProt:P20232 *F references: *F cytology: 35C1--35C1 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-3 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)64j (FBrf0051973) *F PM_dysgenesis_allele: *F l(2)35Cf1 *F l(2)35Cf2 *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank L26091.Genomic sequence *F has a stop codon in middle of gene--this is *F good sequence from 7 reads of 3 different subclones. *F Problem sequence: CGGGATTAGC'TAA'CGTGCCGCG *F =========================================================================== *F \+symbol: vas *F \+formal_name: BG:DS00929.14 *F name: vasa *F synonym: no-relish *F synonym: cgt *F UID: FBgn0003970 *F UID: FBgn0004805 *F references: *F FBrf0047652 == Hay et al., 1988, Cell 55: 577--587 *F FBrf0048768 == Lasko and Ashburner, 1988, Nature 355: 611--617 *F FBrf0051865 == Lasko and Ashburner, 1990, Genes Dev. 4: 905--921 *F FBrf0051573 == Hay et al., 1990, Development 109: 425--433 *F FBrf0052735 == Dorer et al., 1990, Nucleic Acids Res. 18: 5489--5494 *F FBrf0054956 == de Valoir et al., 1991, Proc. Natl. Acad. Sci. USA. 88: *F 2113--2117 *F FBrf0054123 == Schupbach and Wieschaus, 1991, Genetics 129: 1119--1136 *F cytology: 35C1--35C1 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-3 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)A267 (FBrf0048768) *F genefinder: 26(DS00929); 27(DS04929) *F genscan: 285*(3 spans)(DS00929); 131(DS04929) *F match_to: YPD 1430979 e-13(DS00929) Y *F match_to: TR O01378 e-122(DS04929) O (silkworm) *F match_to: SP Q61496 e-96(DS04929) M *F match_to: SP P06634 e-88(DS04929) Y *F match_to: TR O15523 e-87(DS04929) H *F match_to: SP P34689 e-69(DS04929) W *F match_to: TR O22907 e-52(DS04929) A *F EST: LD06084.5'; LD12044.5'; LD17083.5' *F P1: DS00929, DS04929 *F direction: + *F motifs: PS00039 == DEAD-box subfamily ATP-dependent helicases signature. *F homology: species == Caenorhabditis elegans; gene == glh-1 *F homology: species == Mus; gene == 'Vasa'; EMBL: D14859 *F genomic_sequence:X12945; g1054723 *F genomic_sequence:X12946 *F cDNA_sequence:M23560; g158796 *F pr_sequence_SwissProt:P09052 *F pr_sequence_TREMBL:Q24582 *F pr_sequence_PIR:A31922 *F pr_sequence_PIR:S01676 *F ems_allele: *F vas1 *F vas2 *F vas3 *F vas4 *F vas5 *F vas6 *F vasAQB3 *F vasD5 *F vasHE1 *F vasPW72 *F vasQS17 *F vasRG53 *F fs(2)ltoRJ36RJ36 *F vasPH165 *F misc_allele: *F vas3F *F vas4C *F vasAS *F vasKD *F vas88c25 *F P_element_allele: *F vasLYG2 *F EP(2)0812 sequenced *F k07233 sequenced *F PM_dysgenesis_allele: *F vasP808 *F phenotypic_class: female_sterile; non_vital. *F JR_notes: identity of vasa and courgette based on our complementation data *F and those of Montell. *F SM_notes: Translation of 5' from alignment of GenBank X12945 on DS00929, *F but with LD06084.5' at 5' end. Translation of 3' from alignment of GenBank *F M23560 on DS04929. *F k07233 maps to 74616 of DS00929. EP0812 maps to 72346, 2.3kb upstream. *F =========================================================================== *F \+symbol: vig *F \+formal_name: BG:DS00929.13 *F name: vasa intronic gene *F UID: FBgn0024183 *F genefinder: 81 *F genscan: 248 *F product: *F match_to: YPD 927699 e-8 Y *F match_to: TR O24106 e-7 O (tobacco) *F EST: LD07162.5'; LD07162.3'; HL01210.5'; LD20406.5'; *F P1: DS00929 *F direction: - *F Nested_in: vasa *F MA_notes: This is vig (vasa intronic gene) of Kevin Edwards. *F P-elements (EP(2)0812, k07233) that map within vig behave genetically as vasa *F alleles. *F SM_notes: Translation from full-length LD07162. *F =========================================================================== *F \+symbol: BG:DS00929.15 *F \+formal_name: BG:DS00929.15 *F genefinder: *F genscan: 285*(2 spans) *F product: *F match_to: *F EST: *F P1: DS00929 *F direction: + *F Nested_in: vasa *F SM_notes: Translation from 5' edited Genscan (missing 5' end?). *F Paul Lasko's ORF within 3rd vasa intron. Paul's coordinates are *F 76407-77600 and 77894-79528 on the same strand as vasa. *F =========================================================================== *F \+symbol: BG:DS04929.1 *F \+formal_name: BG:DS04929.1 *F genefinder: (11)/68 *F genscan: 302 *F product: *F match_to: GB R18707 e-11 H (Hum EST) *F match_to: GB W71312 e-17 M (Mus EST) *F EST: *F P1: DS04929 *F direction: + *F SM_notes: Translation from Genscan. This is Hawley's Axs-like gene. *F JR_notes: Hawley says in b87e25, but not in A267. *F ========================================================================== *F \+symbol: BG:DS04929.3 *F \+formal_name: BG:DS04929.3 *F genefinder: 54 *F genscan: 461* (3 spans) *F product: Zinc-finger protein (snail-like) *F match_to: TR G3098097 e-35 D *F match_to: SP P51814 e-49 H *F match_to: TR Q62512 e-49 M *F match_to: GB M92443 e-46 H *F match_to: GB D10627 e-46 M *F match_to: SP P47043 e-24 Y *F match_to: GB Z49331 e-24 Y *F match_to: TR Q22874 e-27 W *F match_to: TR O02265 e-27 W *F EST: *F P1: DS04929 *F direction: + *F SM_notes: Translation from 3' edited Genscan. *F ========================================================================== *F \+symbol: stc *F \+formal_name: BG:DS04929.4 *F name: shuttle craft *F synonym: l(2)35Cb *F UID: FBgn0001978 *F references: *F FBrf0085996 == Stroumbakis et al., 1996, Molec. Cell. Biol. 16(1): 192--201 *F FBrf0086298 == Aasland et al., 1995, Trends Biochem. Sci. 20: 56--59 *F cytology: 35C1--35C1 *F cytology_data: Left limit from inclusion within Df(2L)TE35BC-3 (FBrf0038047) *F cytology_data: Right limit from inclusion within Df(2L)TE35B-5 (FBrf0092813) *F genefinder: 114 *F genscan: 461*(6 spans) *F product: DNA-binding-protein-(single-stranded) *F match_to: TR Q18034 e-136 W *F match_to: SP Q12986 e-92 H *F match_to: GB U15306 e-90 H *F match_to: YPD 1301855 e-71 Y *F match_to: SP P53971 e-69 Y *F match_to: GB AA034687 e-9 M (Mus EST) *F match_to: GB AB005237 e-35 A *F EST: LD08727.5'; LD09946.5'; LD02285.5'; LD08763.5'; LD22726.5'; *F LD27525.5'; LD32773.5'; LD25467.5'; LD29364.5'; LD25490.5' *F P1: DS04929 *F direction: + *F motifs: RD-domain protein. *F motifs: Zinc finger, C4HC3 type (PHD finger), protein. *F homology: species == Homo sapiens; gene == 'NF-X1'; EMBL: U15306 *F cDNA_sequence:U09306; g487400 *F pr_sequence_SwissProt:P40798 *F ems_allele: *F stc1 *F stc2 *F stc3 *F stc4 *F stc5 *F P_element_allele: *F stc05441 sequenced this is aka PZ9 *F aberration_associated_allele: *F stc6 (In(2L)dpps22) *F Df(2L)Sco-rv4 hypomorphic *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank U09306. PZ05441 is in *F intron of DS04929.3. Only 9bp of sequence from *F k11112, which maps to DS07851 by in situ, but by sequence matches many Adh *F P1s. *F JR_notes: PZ05441 confirmed as stc allele, but k11112 unlikely to be stc *F allele. 10 excisions of k11112; all are lethal over stc-. *F =========================================================================== *F \+symbol: BG:DS03192.1 *F \+formal_name: BG:DS03192.1 *F genefinder: *F genscan: *F tRNAscan-SE: 66 *F product: transfer RNA:leu *F match_to: *F EST: *F P1: DS03192 *F direction: - *F ========================================================================== *F \+symbol: BG:DS03192.3 *F \+formal_name: BG:DS03192.3 *F genefinder: *F genscan: *F product: *F match_to: *F EST: HL02392.5'; HL02392.3' *F P1: DS03192 *F direction: + *F SM_notes: Translation from full-length HL02392. No good ORF in HL02392 *F cDNA, so short. *F ========================================================================== *F \+symbol: BG:DS03192.2 *F \+formal_name: BG:DS03192.2 *F genefinder: 82/(15) *F genscan: 301 *F product: chaoptin_like *F match_to: GB M19017 e-57 D *F match_to: TR Q18902 e-50 W *F match_to: SP O02833 e-28 H *F match_to: SP P70389 e-22 M *F match_to: YPD 1006714 e-17 Y *F match_to: TR Q42371 e-11 A *F match_to: TR G3075386 e-11 A *F EST: *F P1: DS03192 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS03192.4 *F \+formal_name: BG:DS03192.4 *F genefinder: *F genscan: *F product: *F match_to: *F EST: CK01083.5' *F P1: DS03192 *F direction: - *F Nested_in: BG:DS03192.2 *F SM_notes: Translation from full-length CK01083. No good ORF so short. *F CK01083.3prime sequence doesn't match. No good ORF. *F ========================================================================== *F \+symbol: BG:DS07295.1 *F \+formal_name: BG:DS07295.1 *F genefinder: 35 *F genscan: 66 *F product: Zinc transporter *F match_to: TR Q62941 e-49 O (rat) *F match_to: GB U50927 e-41 O (rat) *F match_to: TR Q22541 e-45 W *F match_to: TR O35149 e-27 M *F match_to: TR P97441 e-39 M *F match_to: TR O14863 e-27 H *F match_to: TR Q99726 e-35 H *F match_to: YPD 1420694 e-10 Y *F EST: GM01103.5'; GM01103.3'; GM10990.5'; GM010990.3'; GM02135.5'; *F GM03441.5'; GM04177.5'; GM07809.5' *F P1: DS03192 (was DS07295) *F direction: - *F SM_notes: Translation from full-length GM01103. Interesting that it is *F only in ovary cDNA library. *F =========================================================================== *F \+symbol: BG:DS07295.4 *F \+formal_name: BG:DS07295.4 *F genefinder: *F genscan: 76 *F product: *F match_to: *F EST: *F P1: DS03192 (was DS07295) *F direction: + *F Nested_in: BG:DS07295.1 *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS07295.2 *F \+formal_name: BG:DS07295.2 *F genefinder: 128 *F genscan: 261 *F product: *F match_to: *F EST: *F P1: DS03192 (was DS07295) *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS07295.3 *F \+formal_name: BG:DS07295.3 *F genefinder: 60 *F genscan: 139 *F product: *F match_to: YPD 486581 e-7 Y *F match_to: YPD 1323271 e-7 Y *F EST: LD09767.5'; LD09767.3'; LD17641.5'; GM09074.5' *F P1: DS03192 (was DS07295), (DS05639) *F direction: - *F SM_notes: Translation from full-length LD09767. *F ========================================================================== *F \+symbol: BG:DS07295.5 *F \+formal_name: BG:DS07295.5 *F genefinder: 26(DS03192) *F genscan: 96(DS03192) *F product: *F match_to: *F EST: *F P1: DS03192 (was DS07295), DS05639 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS05639.1 *F \+formal_name: BG:DS05639.1 *F genefinder: 22(DS03192)/59/26(DS05639) *F genscan: 64(DS03192)/205(DS05639) *F product: *F match_to: *F EST: *F P1: DS03192 (includes DS07295), DS05639, DS07851 *F direction: - *F SM_notes: Translation from genscan--was formerly 3' end of BG:DS07851.11. *F ========================================================================== *F \+symbol: BG:DS07851.11 *F \+formal_name: BG:DS07851.11 *F genefinder: 32(DS07851) *F genscan: 349* (2 spans)(DS07851) *F product: *F match_to: *F EST: GH14032.5' *F P1: DS05639, DS07851 *F direction: - *F SM_notes: Translation from full-length GH14032 on DS07851. *F ========================================================================== *F symbol: rd *F name: reduced *F UID: FBgn0003213 *F references: *F FBrf0092806 == Mohler, 1997, Mini-AIR 1997-02 *F cytology: 35C3+--35C3+ *F cytology_data: Left limit from inclusion within Df(2L)TE35D-15 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)A215 (FBrf0038050) *F spontaneous_allele: *F rd1 *F rds *F gamma_ray_allele: *F rd4 *F rd7 *F rd9 *F misc_allele: *F rdGJ3 *F phenotypic_notes: bristles short and thin. *F phenotypic_class: visible; non_vital. *F JR_notes: Rare male recombinants of gftPZ06430 are either rd+, 35Cc+, gft-, *F ms(2)35Ci- or rd+, 35Cc-, gft-, ms(2)35Ci+ i.e. we have separated rd *F from 35Cc (35Cc is real) and ordered rd, 35Cc and gft (in that order). *F =========================================================================== *F symbol: l(2)35Cc *F name: lethal (2) 35Cc *F UID: FBgn0001979 *F references: *F cytology: 35C3+--35C3+ *F cytology_data: Left limit from inclusion within Df(2L)TE35D-15 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)A263 (FBrf0055544) *F alleles: *F none known; associated with lethality of Df(2L)rd9. *F phenotypic_class: vital. *F JR_notes: Rare male recombinants of gftPZ06430 are either rd+, 35Cc+, gft-, *F ms(2)35Ci- or rd+, 35Cc-, gft-, ms(2)35Ci+ i.e. we have separated rd *F from 35Cc (35Cc is real) and ordered rd, 35Cc and gft (in that order). *F =========================================================================== *F \+symbol: gft *F \+formal_name: BG:DS07851.2 *F name: guftagu *F synonym: l(2)35Cd *F UID: FBgn0001980 *F references: *F FBrf0085597 == Mistry et al., 1996, A. Conf. Dros. Res. 37: 352 *F cytology: 35C3+--35C3+ *F cytology_data: Left limit from inclusion within Df(2L)TE35D-15 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)A263 (FBrf0038050) *F genefinder: 79 *F genscan: 349*(10 spans) *F product: CUL-3 homolog *F match_to: SP Q17391 e-152 W *F match_to: GB U58085 e-134 W *F match_to: TR G3139079 e-176 H *F match_to: TR O22770 e-43 A *F match_to: SP P53202 e-20 Y *F EST: LD10516.3'; LD10516.5'; LD03316.5'; LD03964.5'; LD34096.5'; LD29530.5' *F P1: DS07851 *F direction: - *F ems_allele: *F gft1 *F gft2 *F gft3 *F gft4 *F gft5 *F gft6 *F gft7 *F P_element_allele: *F gft06430 sequenced *F PM_dysgenesis_allele: *F gftd577 *F gamma_induced_allele: *F gftGR18 *F misc_allele: *F gftIK1 *F phenotypic_notes: lethal escapers with unexpanded wings, small eyes. *F phenotypic_class: vital. *F JR_notes: Rare male recombinants of gftPZ06430 are either rd+, 35Cc+, gft-, *F ms(2)35Ci- or rd+, 35Cc-, gft-, ms(2)35Ci+ *F SM_notes: Translation from full-length LD10516. *F =========================================================================== *F \+symbol: BG:DS07851.3 *F \+formal_name: BG:DS07851.3 *F genefinder: 22 *F genscan: *F product: perchloric acid soluble *F match_to: SP P52759 e-17 O (rat) *F match_to: SP P52760 e-23 M *F match_to: SP P52758 e-22 H *F match_to: SP Q10121 e-18 W *F match_to: SP P40185 e-10 Y *F match_to: SP P40037 e-7 Y *F EST: GM01181.3'; GM01181.5' *F P1: DS07851 *F direction: - *F SM_notes: Translation from full-length GM01181. *F =========================================================================== *F \+symbol: BG:DS07851.4 *F \+formal_name: BG:DS07851.4 *F genefinder: 22 *F genscan: 65 *F product: *F match_to: *F EST: *F P1: DS07851 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS07851.8 *F \+formal_name: BG:DS07851.8 *F genefinder: 22 *F genscan: 59 *F product: *F match_to: *F EST: *F P1: DS07851 *F direction: - *F SM_notes: Translation from Genscan. maps close to ms(2)02316=ms(2)35Ci *F (27661-29308 vs 30208) *F JR_notes: ms(2)46AB02316 maps within A263, osp18 and TE35D-6 but not *F noc11, nBR129, TE35D-2 or TE35D-21. *F Zuker's ms 14-107 maps to the same place i.e. it is ms over A263 *F (Df(2L)34E5-F1;35C3-5) and osp18. *F ms(2)46AB02316 is homozygous male fertile and fertile over ms *F 14-107, but could be a weak allele of the same gene. *F ms 14-107 is homozygous ms. *F ========================================================================== *F \+symbol: ms(2)35Ci *F \+formal_name: BG:DS07851.10 *F synonym: 14-107 *F genefinder: *F genscan: (35) *F product: *F match_to: *F EST: *F P1: DS07851 *F direction: - *F P_element_allele: *F ms(2)35Ci02316 *F SM_notes: Translation from Genscan. ms(2)02316 maps in intron of gene *F prediction (29377-33910 vs 30208) *F BG:DS07851.8 maps close to ms(2)02316=ms(2)35Ci (27661-29308 vs 30208) *F ms(2)02316 maps in intron of BG:DS07851.10 gene prediction(29377-33910 vs *F 30208) *F JR_notes: ms(2)46AB02316 maps genetically to the same region as the Zuker *F ms 14-107 i.e. it is ms over A263 (Df(2L)34E5-F1;35C3-5) and osp18 *F ms(2)46AB02316 is homozygous male fertile and fertile over ms *F 14-107, but could be a weak allele of the same gene.ms 14-107 is homozygous ms. *F JR_notes: Zuker EMS male-sterile mapped to gft-esg interval *F ms(2)46AB02316 maps within A263, osp18 and TE35D-6 but not *F noc11, nBR129, TE35D-2 or TE35D-21.Zuker's ms 14-107 maps to the same place *F i.e. it is ms over A263 *F (Df(2L)34E5-F1;35C3-5) and osp18. *F ms(2)46AB02316 is homozygous male fertile and fertile over ms *F 14-107, but could be a weak allele of the same gene. *F ms 14-107 is homozygous ms. *F ========================================================================== *F \+symbol: BG:DS07851.5 *F \+formal_name: BG:DS07851.5 *F genefinder: 31 *F genscan: 108 *F product: *F match_to: GB AA864782 e-8 H (Homo EST) *F match_to: TR Q94547 e-19 D *F match_to: TR Q10923 e-12 W *F match_to: YPD 1431262 e-7 Y *F EST: *F P1: DS07851 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS07851.6 *F \+formal_name: BG:DS07851.6 *F genefinder: (20) *F genscan: 60 *F product: *F match_to: *F EST: *F P1: DS07851 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F symbol: l(2)35Cg *F JR_notes: Predicted from deletion o/laps. *F ========================================================================== *F \+symbol: esg *F \+formal_name: BG:DS07851.7 *F name: escargot *F synonym: l(2)35Ce *F synonym: shof *F synonym: l(2)k08225 *F UID: FBgn0001981 *F references: *F FBrf0057128 == Whiteley et al., 1992, Mech. Dev. 36: 117--127 *F FBrf0055837 == Smith et al., 1992, Development 116(4): 1033--1039 *F FBrf0058108 == Hayashi et al., 1993, Development 118(1): 105--115 *F references l(2)k08225: *F FBrf0058618 == Torok et al., 1993, Genetics 135(1): 71--80 *F FBrf0080244 == Mechler, 1994, J. Biosci., Bangalore 19(5): 537--556 *F cytology: 35C4--35C5 *F cytology_data: Left limit from inclusion within Df(2L)TE35D-13 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)TE35B-1 (FBrf0091422) *F motifs: PS00028 == Zinc finger, C2H2 type, domain. *F genefinder: 65 *F genscan: 158 *F match_to: TR P97469 e-50 M *F match_to: GB U79550 e-49 M *F match_to: TR G2832266 e-50 H *F match_to: TR E1297902 e-28 W *F match_to: SP P47043 e-12 Y *F match_to: YPD 1008197 e-17 Y *F EST: *F P1: DS07851 *F direction: + *F homology: species == Homo sapiens; gene == IMAGE: 198754; EMBL: R95170; *F DRES: 51 *F homology: species == Xenopus laevis; gene == 'XSNA'; SWP: P19382 *F cDNA_sequence:M83207; g157367 *F pr_sequence_SwissProt:P25932 *F pr_sequence_PIR:S33639 *F ems_allele: *F esg35Ce-1 *F esg35Ce-2 *F esg35Ce-3 *F P_element_allele: *F esg05729 *F esg05730 sequenced *F esg07082 sequenced *F esgB7-2-22 *F esgG66 *F esgM5-4 *F esgP1 *F esgP2 *F esgk00109 *F esgk00418 *F esgk00606 *F esgk01107 *F esgk01502 *F esgk02706 *F esgk02811 *F k03506 *F esgk04408 *F esgk04802 *F esgk05210 *F esgk05517 *F esgk05818 *F esgk07808 *F esgk07902 *F esgk08104 *F esgk08418 *F esgk08505 *F esgk08610 *F esgk08910 *F esgk10811 cluster: k10817* in situ & seq at 50C, hit&run *F esgk14104 cluster: k14108 *F esgk14811 *F esgk15418 *F esgk15818 *F esgk16117 *F PZ06346 sequenced this a second site; PZ06346 per se Stat92E allele *F l(2)k08225k08225 sequenced *F n(2)k09122 sequenced *F n(2)k04509 sequenced *F n(2)k06817b sequenced *F n(2)k05652 sequenced *F n(2)k03802 sequenced *F n(2)k04207 sequenced *F n(2)k03503 sequenced *F n(2)k15310 sequenced *F EP(2)0633 sequenced *F EP(2)0683 sequenced *F EP(2)0684 sequenced *F EP(2)2408 sequenced *F EP(2)2009 sequenced *F EP(2)2159 sequenced *F n(2)k02405 sequenced *F l(2)k07018 sequenced *F l(2)k06211 *F k03012 *F k17032 *F P_element_derivative_allele: *F esgL2 *F esgL3 *F esgL6 *F esgL7 *F PM_dysgenesis_allele: *F esg148 *F esg80 *F esg94 *F esgflg *F aberration_associated_allele: *F esgGW2 (Df(2L)TE35D-2) *F esgGW13 (Df(2L)TE35D-13) *F esgGW21 (Df(2L)TE35D-21) *F esgdgl (T(Y;2)J165) *F k15711 sequenced this is a deletion 35Bb-esg, hypomorphic esg *F phenotypic_class: vital. *F phenotypic_notes: l(2)k08225 = neoplastic brain phenotype, vital. *F JR_notes: k15310, k09122 and k03503 have no esg phenotype - they are viable *F & fertile - over esg- *F JR_notes: k08225 viable and fertile with A48, b84a7 and r10 (reduced female *F fert with A48). Worrying because Torok et al states: P-element excision *F demonstrates the insertion to be the cause of lethality. *F SM_notes: Translation from alignment of GenBank M83207. *F ========================================================================== *F \+symbol: BG:DS03023.4 *F \+formal_name: BG:DS03023.4 *F genefinder: *F genscan: 194 *F product: *F match_to: *F EST: *F P1: DS03023 *F direction: - *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: wor *F \+formal_name: BG:DS03023.1 *F name: lethal (2) 35Da *F name: worniu *F synonym: l(2)35Da *F synonym: schnecke *F UID: FBgn0001983 *F references: *F cytology: 35D1--35D1 *F cytology_data: Left limit from inclusion within Df(2L)TE35D-2 (FBrf0051973) *F cytology_data: Right limit from complementation mapping against Tp(2;2)Sco *F (citation unavailable) *F product: Zinc-finger protein *F genefinder: 73 *F genscan: 147 *F match_to: GB M83207 e-51 D *F match_to: SP P25932 e-67 D *F match_to: TR G2832266 e-50 H *F match_to: TR P97469 e-50 M *F match_to: TR E1297902 e-28 W *F match_to: SP P47043 e-12 Y *F match_to: YPD 1008197 e-15 Y *F EST: *F P1: DS03023 *F direction: - *F ems_allele: *F l(2)35Da1 *F l(2)35Da2 *F l(2)35Da3 *F l(2)35Da4 *F misc_allele: *F l(2)35DaAB108 *F l(2)35DaAB114 *F l(2)35DaAB48 *F l(2)35DaAS79 *F phenotypic_notes: disrupted polarity in larval cuticle belts. *F phenotypic_class: vital. *F SM_notes: Translation from alignment of cDNA sequence from T. Ip (was *F called zfp). *F =========================================================================== *F symbol: l(2)35Ch *F synonym: l(2) *F phenotypic_class: lethal *F JR_notes: No alleles; overlapping deletion phenotype; predicted between *F l(2)35Da and sna. *F =========================================================================== *F \+symbol: BG:DS03023.2 *F \+formal_name: BG:DS03023.2 *F genefinder: 30 *F genscan: 127 *F product: *F match_to: LD26668.5' e-8 *F match_to: TR E1246803 e-53 W *F match_to: SP Q04371 e-22 Y *F match_to: GB AA450460 e-15 M (Mus EST) *F match_to: GB AA223779 e-7 H (Hum EST) *F EST: *F P1: DS03023 *F direction: + *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: sna *F \+formal_name: BG:DS01845.1 *F name: snail *F synonym: l(2)35Db *F UID: FBgn0003448 *F references: *F FBrf0053832 == Alberga et al., 1991, Development 111: 983--992 *F FBrf0047066 == Boulay et al., 1987, Nature 330: 395--398 *F FBrf0064312 == Boulay, 1988, Ph.D. Thesis, Louis Pasteur, Strasbourg., *F 107pp. *F FBrf0056115 == Ip et al., 1992, Genes Dev. 6: 1728--1739 *F FBrf0057499 == Sommer et al., 1992, Proc. natn. Acad. Sci. USA. 89(22): *F 10782--10786 *F FBrf0046065 == Carroll et al., 1987, Development 99: 327--332 *F FBrf0063478 == Grau et al., 1984, Genetics 108: 347--360 *F FBrf0057526 == Kasai et al., 1992, Proc. natn. Acad. Sci. USA. 89: *F 3414--3418 *F FBrf0051581 == Leptin and Grunewald, 1990, Development 110(1): 73--84 *F FBrf0054033 == Leptin, 1991, Genes Dev. 5: 1568--1576 *F FBrf0054034 == Rao et al., 1991, Genes Dev. 5: 1577--1588 *F FBrf0051595 == Sargent and Bennett, 1990, Development 109: 967--973 *F cytology: 35D1--35D2 *F cytology_data: Left limit from inclusion within Df(2L)Scorv16 *F (FBrf0039455) *F cytology_data: Right limit from inclusion within Df(2L)b80e4 (FBrf0051973) *F product: transcription-factor *F product: DNA-binding-protein *F genefinder: 73 *F genscan: 136 (2 spans) *F match_to: TR O16139 e-55 O (sea urchin) *F match_to: TR P97469 e-51 M *F match_to: GB U79550 e-50 M *F match_to: TR G2832266 e-51 H *F match_to: TR E1297902 e-31 W *F match_to: SP P47043 e-15 Y *F match_to: YPD 1008197 e-15 Y *F EST: *F P1: DS01845 *F direction: - *F motifs: PS00028 == Zinc finger, C2H2 type, domain. *F homology: species == Mus; gene == Sna; MGD: MRK-14460 MEDLINE: 93130772 *F cDNA_sequence:Y00288; g8630 *F pr_SwissProt:P08044 *F pr_sequence_PIR:S06222 *F pr_sequence_PIR:C46363 *F ems_allele: *F sna1 *F sna2 *F sna3 *F sna4 *F sna9 *F sna10 *F sna11 *F sna12 *F sna13 *F sna14 *F sna15 *F sna16 *F sna17 *F sna18 *F X_ray_allele: *F sna5 *F sna6 *F sna8 *F sna19 *F sna20 *F snaS1 *F misc_allele: *F snaAM15 *F snary40 *F aberration_associated_allele: *F sna7 (T(2;3)sna7) *F snaH50 (T(2;3)H50) *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank Y00288. *F =========================================================================== *F \+symbol: Tim17 *F \+formal_name: BG:DS01845.2 *F name: Translocase inner membrane 17 *F references: *F FBrf0090402 *F products: preprotein translocase of the inner mitochondrial membrane *F genefinder: (19) *F genscan: 80 *F match_to: LD02976.5' e-54 D *F match_to: SP Q99595 e-45 H *F match_to: GB X97544 e-43 H *F match_to: GB AA014040 e-40 M (Mus EST) *F match_to: YPD 1015553 e-32 Y *F match_to: SP P39515 e-31 Y *F match_to: TR G2702415 e-30 W *F match_to: GB T45278 e-21 A *F match_to: CK01513.3' e-26 D *F EST: *F P1: DS01845 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR products from GH(4) *F and LP(2) libraries. *F =========================================================================== *F \+symbol: lace *F \+formal_name: BG:DS01845.3 *F name: lace *F synonym: l(2)35Dc *F UID: FBgn0002524 *F references: *F FBrf0064312 == Boulay, 1988, Ph.D. Thesis, Louis Pasteur, Strasbourg., *F 107pp. *F cytology: 35D3--35D3 *F cytology_data: Left limit from in situ hybridisation (FBrf0067338) *F cytology_data: Right limit from complementation mapping against T(Y;2)b8 *F (citation unavailable) *F genefinder: 11/67 *F genscan: 147 *F product: serine palmitoyl transferase *F match_to: GB X95642 e-128 M *F match_to: TR P97363 e-130 M *F match_to: TR O15270 e-129 H *F match_to: TR D1026382 e-129 H *F match_to: TR Q20375 e-109 W *F match_to: YPD 798913 e-97 Y *F match_to: SP P40970 e-90 Y *F EST: LD17449.5'; LD17449.3' *F P1: DS01845 *F direction: + *F spontaneous_allele: *F lace1 *F ems_allele: *F lace2 *F lace4 *F lace5 *F lace6 *F lace7 *F lace8 *F lace9 *F lace10 *F lace11 *F lace12 *F lace14 *F lace15 *F lace16 *F lace17 *F P_element_allele: *F lacek00706 *F lacek02303 *F lacek05305 sequenced *F lacek07501 *F lacek08208 *F lacek11309 *F misc_allele: *F laceIRX6 *F lacea2 *F aberration_associated_allele: *F lace3 (T(Y;2)b8) *F phenotypic_notes: weak alleles escape with supernumerary wing vein fragments. *F phenotypic_class: vital. *F SM_notes: Translation from full-length LD17449. *F ========================================================================== *F \+symbol: BG:DS04862.2 *F \+formal_name: BG:DS04862.2 *F genefinder: *F genscan: 66 *F product: *F match_to: *F EST: *F P1: DS04862 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR products from GM(1) *F and GH(1) libraries. *F ========================================================================== *F \+symbol: kek3 *F \+formal_name: BG:DS04862.1 *F genefinder: 110 *F genscan: 252 *F product: kek1-like *F match_to: GB U42767 e-25 D *F match_to: TR P91643 e-68 D *F match_to: TR P70193 e-17 M *F match_to: TR O15335 e-14ry H *F match_to: TR O16781 e-9 W *F EST: GH12215.5', GH14053.5' *F P1: DS04862 *F direction: + *F JR_notes: From Jean-Marc's data Df(2L)75c removes this gene. Duffy is *F going to make 75c, EGFR- recombinants to see whether (like kek1) kek3 *F rescues/suppresses the various EGFR phenotypes. *F SM_notes: Translation 5' from Genscan/genefinder, middle and 3' from full *F sequence of GH12215. This clone not full-length? 5' end has kek homology. *F ========================================================================== *F \+symbol: BG:BACR44L22.1 *F \+formal_name: BG:BACR44L22.1 *F genefinder: 25 *F genscan: 64 *F product: peptidase family M12A (Zn metalloprotease); astacin subfamily *F match_to: SP P55112 e-33 W *F match_to: SP Q16820 e-30 H *F match_to: SP Q61847 e-26 M *F EST: *F P1: BACR44L22 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR products from GH(2) *F and LP(5) libraries. *F ========================================================================== *F \+symbol: BG:BACR44L22.8 *F \+formal_name: BG:BACR44L22.8 *F genefinder: *F genscan: 110* (2 spans) *F product: peptidase family M12A (Zn metalloprotease); astacin subfamily *F match_to: SP P55112 e-26 W *F match_to: SP Q16820 e-19 H *F match_to: SP P55113 e-19 W *F match_to: TR G238981 e-19 H *F match_to: SP P28825 e-15 M *F EST: *F P1: BACR44L22 *F direction: - *F SM_notes: Translation from edited Genscan (two spans). Kerrie reports PCR *F product from GH(1) library. *F ========================================================================== *F \+symbol: BG:BACR44L22.2 *F \+formal_name: BG:BACR44L22.2 *F genefinder: 23 *F genscan: 110* (2 spans) *F product: peptidase family M12A (Zn metalloprotease); astacin subfamily *F match_to: SP P55112 e-31 W *F match_to: SP Q16820 e-30 H *F match_to: SP P55113 e-19 W *F match_to: TR G238981 e-24 H *F match_to: SP P28826 e-23 M (rat) *F EST: *F P1: BACR44L22 *F direction: - *F SM_notes: Translation from edited Genscan (two spans). Kerrie reports PCR *F products from GH(2) and LP(2) libraries. *F ========================================================================== *F \+symbol: BG:BACR44L22.3 *F \+formal_name: BG:BACR44L22.3 *F genefinder: *F genscan: 53 *F product: peptidase family M12A (Zn metalloprotease); astacin subfamily *F match_to: SP P55112 e-33 W *F match_to: TR G238981 e-27 H *F match_to: SP Q61847 e-23 M *F EST: *F P1: BACR44L22 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR products from GH(1) *F and LP(1) libraries. *F ========================================================================== *F \+symbol: BG:BACR44L22.4 *F \+formal_name: BG:BACR44L22.4 *F genefinder: *F genscan: (29) *F product: peptidase family M12A (Zn metalloprotease); astacin subfamily *F match_to: SP P55112 e-28 W *F match_to: TR G238981 e-25 H *F match_to: SP P28825 e-22 M *F EST: *F P1: BACR44L22 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR product from GH(1) *F library. *F ========================================================================== *F \+symbol: BG:BACR44L22.5 *F \+formal_name: BG:BACR44L22.5 *F genefinder: *F genscan: 51 *F product: *F match_to: *F EST: *F P1: BACR44L22 *F direction: - *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:BACR44L22.6 *F \+formal_name: BG:BACR44L22.6 *F genefinder: 25 *F genscan: 59 (2 spans) *F product: peptidase family M12A (Zn metalloprotease); astacin subfamily *F match_to: SP P55112 e-35 W *F match_to: TR Q18439 e-23 W *F match_to: TR G238981 e-25 H *F match_to: SP Q16820 e-23 H *F match_to: SP Q61847 e-21 M *F EST: *F P1: BACR44L22 *F direction: + *F SM_notes: Translation from 5' edited Genscan and homology. *F ========================================================================== *F \+symbol: BG:DS07108.4 *F \+formal_name: BG:DS07108.4 *F genefinder: 46(BACR44L22)/20(DS07108) *F genscan: 155(BACR44L22)/71(DS07108) *F product: *F match_to: SP P02750 e-9 H *F match_to: TR D1033423 e-8 M (rat) *F match_to: TR G2668616 e-7 W *F EST: LP11031.5', LP08921.5' *F P1: BACR44L22, DS07108 *F direction: + *F SM_notes: Translation from Genscan at 5' and complete sequence of LP11031 *F at 3' (clone not full-length?). *F ========================================================================== *F \+symbol: BG:DS07108.2 *F \+formal_name: BG:DS07108.2 *F genefinder: 128 *F genscan: 309 *F product: DHP-sensitive Ca-channel *F match_to: TR O08532 e-51 M *F match_to: TR O08533 e-51 M *F match_to: TR O08534 e-51 M *F match_to: TR O08535 e-51 M *F match_to: TR O08536 e-51 M *F match_to: SP P54289 e-51 H *F match_to: TR G2781441 e-53 H *F match_to: SP P34374 e-24 W *F EST: *F P1: DS07108 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR products from LD *F A8-F8 (2) library. *F ========================================================================== *F \+symbol: BG:DS07108.1 *F \+formal_name: BG:DS07108.1 *F genefinder: *F genscan: 75 *F product: serine protease *F match_to: TR P91817 e-40 O (horseshoe crab) *F match_to: GB D87214 e-39 O (horseshoe crab) *F match_to: SP P21845 e-25 M *F match_to: SP Q05319 e-25 D *F match_to: SP P40313 e-23 H *F match_to: TR Q17800 e-19 W *F EST: *F P1: DS07108 *F direction: + *F JR_notes: Jean-Marc Reichhart has been assaying putative overlapping *F deletions in the lace-CycE *F interval for the absence of the serine proteases (DS07108.1 and .5). He *F has found that only 2 combinations Df(2L)75c/TE35D-17 and 75c/TE35D-19 *F remove these genes. None of the other 9 coming in from the left remove *F them and neither does the one other coming in from the right (TE35D-3). *F (This fits with the lace-DS07108.1 and DS07108.5-CycE distances.) *F SM_notes: Translation from Genscan. *F ========================================================================== *F \+symbol: BG:DS07108.5 *F \+formal_name: BG:DS07108.5 *F P_element_allele: *F PZ09259 sequenced *F genefinder: *F genscan: 25 *F product: serine protease *F match_to: TR P91817 e-34 O (horseshoe crab) *F match_to: GB D87214 e-33 O (horseshoe crab) *F match_to: TR E1319203 e-25 M *F match_to: SP P05981 e-22 H *F EST: *F P1: DS07108 *F direction: + *F SM_notes: Translation from Genscan. *F PZ09259 maps 3'(50257-50281) to this gene(37545-38529); allele ? *F ========================================================================== *F \+symbol: CycE *F \+formal_name: BG:DS07108.3 *F name: Cyclin E *F synonym: l(2)35Dd *F UID: FBgn0010382 *F references: *F FBrf0080145 == Kania et al., 1995, Genetics 139(4): 1663--1678 *F FBrf0073016 == Edgar, 1994, Curr. Biol. 4(6): 522--524 *F FBrf0077697 == Orr-Weaver, 1994, Trends Genet. 10(9): 321--327 *F FBrf0065548 == Richardson et al., 1993, Development 119(3): 673--690 *F FBrf0068557 == Knoblich et al., 1994, Cell 77(1): 107--120 *F FBrf0073011 == Duronio and O'Farrell, 1994, Development 120(6): 1503--1515 *F FBrf0073015 == Edgar et al., 1994, Development 120(11): 3131--3143 *F FBrf0081968 == Duronio and O'Farrell, 1995, Genes Dev. 9(12): 1456--1468 *F FBrf0082560 == Sauer et al., 1995, Genes Dev. 9(11): 1327--1339 *F FBrf0084309 == Richardson et al., 1995, Development 121(10): 3371--3379 *F FBrf0073016 == Edgar, 1994, Curr. Biol. 4(6): 522--524 *F FBrf0084488 == Weigmann and Lehner, 1995, Development 121(11): 3713--3721 *F FBrf0077261 == Finley and Brent, 1994, Proc. natn. Acad. Sci. USA. *F 91(26): 12980--12984 *F FBrf0091052 == Edgar and Lehner, 1996, Science 274(5293): 1646--1652 *F FBrf0092831 == Follette, , Cell 88: 309--314 *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)el80f1 (FBrf0039455) *F cytology_data: Right limit from inclusion within Df(2L)A377 (FBrf0038050) *F genefinder: 94 *F genscan: 193/(33) *F product: cyclin *F match_to: SP P47794 e-60 O (zebrafish) *F match_to: SP P24864 e-57 H *F match_to: TR Q61457 e-57 M *F match_to: TR O01501 e-30 W *F match_to: SP P30283 e-18 Y *F EST: LD17578.5'; LD12856.5'; LD13027.5'; LD15627.5'; LD02508.5'; *F LD16315.5'; LD16315.5'; LD19554.5'; LD22682.5'; LD24885.5'; LD26520.5'; *F LD27646.5'; LD29877.5'; LD29878.5' *F P1: DS07108, DS09217 *F direction: - *F motifs: Cyclins signature protein. *F homology: species == Homo sapiens; gene == CCNE; GDB: 128967; OMIM: 123837 *F cDNA_sequence:X75026; g429166 *F cDNA_sequence:X75027; g429168 *F pr_sequence_SwissProt:P54733 *F pr_sequence_TREMBL:Q24479 *F pr_sequence_PIR:S41755 *F pr_sequence_PIR:S41756 *F ems_allele: *F CycE2 *F CycE3 *F CycEAR95 *F P_element_allele: *F CycE01672 *F CycE05206 sequenced *F CycE05277 *F CycE05278 *F CycE10427 *F CycE25-14 *F CycEk00807 *F CycEk02602 sequenced *F CycEk05007 sequenced *F CycEk09109 *F misc_allele: *F CycE4 *F CycE50-2 *F TE_insert_allele: *F CycE5 (Tp(1;2)TE35D) *F aberration_associated_allele: *F CycE1 (T(2;3)G16) *F phenotypic_class: vital; hypomorphic alleles female sterile. *F SM_notes: Translation for type 2 from alignment of GenBank X75027 on *F DS09217(1-284) *F and DS07108, type 1 from GenBank X75026 on DS07108. *F =========================================================================== *F \+symbol: BG:DS09217.1 *F \+formal_name: BG:DS09217.1 *F genefinder: 36 *F genscan: 60 *F product: *F match_to: (TR Q23597 e-6 W) *F EST: HL02152.5'; GH01660.5' *F P1: DS09217 *F direction: - *F SM_notes: Translation from full-length GH01660. Evidence for alternative *F splicing *F since second exon of HL02152.5' extends further 3' than that of GH01660. *F =========================================================================== *F \+symbol: l(2)35Df *F \+formal_name: BG:DS09217.2 *F name: lethal (2) 35Df *F UID: FBgn0001986 *F references: *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35D-1 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)b88c75 (FBrf0092813) *F ems_allele: *F l(2)35Df1 *F l(2)35Df2 *F l(2)35Df3 *F l(2)35Df4 *F l(2)35DfHL58 *F P_element_allele: *F l(2)35Dfk14423 sequenced k14422 cluster *F genefinder: 161 (2 spans) *F genscan: 404 *F product: MTR4 ATP-dependent RNA helicase *F match_to: SP P42285 0 H *F match_to: GB D29641 0 H (KIAA0052) *F match_to: SP Q15477 e-141 H *F match_to: SP P47047 e-264 Y *F match_to: GB Z49325 e-262 Y *F match_to: SP Q23223 e-243 W *F match_to: TR G2944423 e-133 M *F match_to: TR O04538 e-40 A *F EST: LD10786.5'; LD10786.3'; GM09051.5'; LD02559.5'; LD05183.5'; *F LD12317.5'; LD12395.5'; LD20038.5'; LD27110.5'; LD28043.5' *F P1: DS09217 *F direction: + *F phenotypic_class: vital; escapers HL58 female sterile, small bristles. *F SM_notes: Translation from full-length LD10786 and LD27110.5', because *F something *F fishy with LD10786 (has intron where other cDNAs from ESTs don't). *F P is inserted at 6949 of DS09217. The cDNA for BG:DS09217.1 is GH01660, *F and the full-length sequence is from 5693 to 4243 of DS09217 (reverse strand). *F The cDNA for BG:DS09217.2 is LD10786, and its full length sequence is from *F 6962 to 10532 (forward strand). However, the EST LD02559 extends even further *F 5', and starts at 6936. *F =========================================================================== *F symbol: l(2)35Di *F name: lethal (2) 35Di *F UID: FBgn0001989 *F references: *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35D-1 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)b88c75 (FBrf0092813) *F ems_allele: *F l(2)35DiRAR8 *F phenotypic_notes: escapers with held out wings. *F phenotypic_class: vital. *F =========================================================================== *F \+symbol: Gli *F \+formal_name: BG:DS09217.3 *F name: Gliotactin *F synonym: l(2)35Dg *F UID: FBgn0001987 *F references: *F FBrf0081345 == Auld et al., 1995, Cell 81(5): 757--767 *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35D-1 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)b88c75 (FBrf0092813) *F product: gliotactin *F product: serine esterase \like *F genefinder: 100 *F genscan: 216 (7 spans) *F match_to: TR Q62888 e-53 O (rat) *F match_to: GB U22952 e-46 O (rat) *F match_to: TR Q20826 e-47 W *F match_to: SP P22303 e-43 H *F match_to: SP P21836 e-43 M *F match_to: LD07724.5' e-8; LD02542.5' e-6; LD30351.5' e-5 *F EST: *F P1: DS09217 *F direction: - *F motifs: Transmembrane domain protein. *F cDNA_sequence:L39083; g899062 *F pr_sequence_TREMBL:Q24350 *F pr_sequence_PIR:A56920 *F ems_allele: *F Gli1 *F Gli2 *F Gli3 *F Gli4 *F GliRAR77 *F P_element_allele: *F GliAE2 sequenced Auld *F GliJ29 sequenced Auld *F GlirL82 sequenced Auld *F k09033 sequenced *F EP(2)2322 sequenced *F EP(2)2416 sequenced *F EP(2)2306 sequenced *F EP(2)2463 sequenced *F EP(2)2615 sequenced *F P_element_derivative_allele: *F GliAE2&Dgr;45 *F GliAE2&Dgr;4a *F GliAE2&Dgr;4b *F GliAE2&Dgr;6a *F GliJ29&Dgr;7b *F phenotypic_class: vital. *F JR_notes: k09033 viable and fertile. *F SM_notes: Translation from alignment of GenBank L39083. Gli from 17386-10808. *F EP2322, EP2416 at 17422, EP2306, EP2463, & EP2615 at 17429, l(2)k09033 at *F 17487. *F =========================================================================== *F \+symbol: BG:DS09217.4 *F \+formal_name: BG:DS09217.4 *F genefinder: 64 *F genscan: 105 *F product: *F match_to: SP P46554 e-52 W *F match_to: GB AA461616 e-26 H (Hum EST) *F match_to: TR G3258571 e-16 A *F match_to: YPD 927710 e-8 Y *F match_to: GB AA118474 e-17 M (Mus EST) *F EST: LD08227.3'; LD08227.5' *F P1: DS09217 *F direction: + *F JR_notes: could be 35Di, 35De or 35Dh (see next record). *F SM_notes: Translation from full-length LD08227. *F =========================================================================== *F \+symbol: l(2)35Ea *F \+formal_name: BG:DS09217.5 *F name: lethal (2) 35Ea *F UID: FBgn0001990 *F references: *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35BC-34 *F (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)n78l3 (FBrf0051973) *F genefinder: 67 *F genscan: 137 *F product: Zinc finger protein *F match_to: TR D1024956 e-27 H *F match_to: TR Q62516 e-24 M *F match_to: TR Q22874 e-12 W *F match_to: TR O02265 e-12 W *F match_to: SP P47043 e-8 Y *F EST: LD02957.3'; LD02957.5'; LD22579.5'; LD22579.3' *F P1: DS09217 *F direction: - *F P_element: PZ05271 sequenced Gates *F ems_allele: *F l(2)35EaRAR14 *F misc_allele: *F l(2)35EaAM11 *F phenotypic_class: vital. *F SM_notes: Translation from full-length LD02957. *F Julie Gates in Thummel's lab sequenced l(2)05271 by plasmid *F rescue, first bp of 275bp fragment (insertion site) maps to 22957 *F (276=22638), 281bp upstream from start of LD02957 (22676-21313) and 5bp *F downstream from the start of LD22579.5' (22962-22362). *F JR_notes: 05271 viable and fertile over deletions of 34-36, including 35D, *F so P not associated with lethality in strain. *F All but one of the excisions and male recombinants are viable over r10; *F exception male recombinant is l(2)35Ea. *F Some excisions over progenitor crippled legs and disrupted wings, therefore *F DS09217.5 =l(2)35Ea and PZ05271 has a weak crippled leg and reduced wing *F phenotype when heterozygous with a deletion. *F =========================================================================== *F \+symbol: BG:DS09217.6 *F \+formal_name: BG:DS09217.6 *F genefinder: 97 *F genscan: 262 *F product: Ku ATP-dep. DNA helicase II, 86kD subunit *F match_to: SP P27641 e-20 M *F match_to: SP P13010 e-15 H *F EST: *F P1: DS09217 *F direction: - *F SM_notes: Translation from Genefinder. Kerrie reports hits from LD *F A8-F8(6) library. *F JR_notes: could be 35Di, 35De or 35Dh (see previous record). *F ========================================================================== *F symbol: l(2)35De *F name: lethal (2) 35De *F UID: FBgn0001985 *F references: *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35D-1 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)TE35BC-34 (FBrf0051973) *F aberration_associated_allele: *F l(2)35De1 (In(2L)NS) *F phenotypic_class: vital. *F status: uncertain. *F =========================================================================== *F symbol: l(2)35Dh *F name: lethal (2) 35Dh *F UID: FBgn0001988 *F references: *F cytology: 35D7--35D7 *F cytology_data: Left limit from non-inclusion within Df(2L)TE35BC-34 *F (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)n78l3 (FBrf0051973) *F ems_allele: *F l(2)35DhAS64 *F phenotypic_class: vital. *F =========================================================================== *F \+symbol: BG:DS02252.3 *F \+formal_name: BG:DS02252.3 *F genefinder: *F genscan: 376 *F product: ypt6 suppressor *F match_to: YPD 577221 e-7 Y *F EST: *F P1: DS02252 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR product from GH(1) *F library. *F ========================================================================== *F \+symbol: BG:DS02252.4 *F \+formal_name: BG:DS02252.4 *F genefinder: *F genscan: 71 *F product: *F match_to: SP P12036 e-8 H *F EST: *F P1: DS02252 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR product from GH(1) *F library. *F ========================================================================== *F \+symbol: BG:DS02252.1 *F \+formal_name: BG:DS02252.1 *F genefinder: 48 *F genscan: 129 *F product: *F match_to: *F EST: *F P1: DS02252 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR product from GH(2) *F and LP(3) libraries. *F ========================================================================== *F \+symbol: BG:DS02252.2 *F \+formal_name: BG:DS02252.2 *F genefinder: 40(DS02252)/61(DS00365) *F genscan: 86(DS02252)/189(DS00365) *F product: tektin A1 *F match_to: GB M97188 e-9(DS02252)/e-50(DS00365) O (sea urchin) *F match_to: TR Q26623 e-8(DS02252)/e-37(DS00365) O (sea urchin) *F match_to: TR G3005677 e-31(DS00365) H *F match_to: GB AA108986 e-8(DS02252)/e-18(DS00365) M (Mus EST) *F match_to: TR Q21641 e-14(DS00365) W *F EST: *F P1: DS02252, DS00365 *F direction: + *F SM_notes: Translation from Genscan on DS00365. Kerrie reports PCR products *F from GH(3) and LP(2) libraries. *F ========================================================================== *F \+symbol: BG:DS00365.1 *F \+formal_name: BG:DS00365.1 *F genefinder: (14) *F genscan: 96 *F product: aminopeptidase N *F match_to: SP P15684 e-52 O (rat) *F match_to: SP P15144 e-48 H *F match_to: SP P97449 e-43 M *F match_to: TR Q21673 e-34 W *F match_to: SP P32454 e-31 Y *F EST: *F P1: DS00365 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR product from GH(3) *F and LP(2) libraries. *F ========================================================================== *F \+symbol: BG:DS00365.2 *F \+formal_name: BG:DS00365.2 *F genefinder: 38 *F genscan: 253 *F product: alpha-macroglobulin *F match_to: TR E1247576 e-107 W *F match_to: TR E1247575 e-107 W *F match_to: TR Q60486 e-80 O (guinea pig) *F match_to: SP P20742 e-76 H *F match_to: GB X54380 e-75 H *F match_to: SP Q61838 e-66 M *F match_to: HL07316.5' e-23; HL04948.5' e-21 *F EST: *F P1: DS00365 *F direction: - *F M *F SM_notes: Translation from Genscan. Kerrie reports PCR product from LP(4) *F library. *F ========================================================================== *F \+symbol: BG:DS00365.3 *F \+formal_name: BG:DS00365.3 *F genefinder: 51 *F genscan: 170 *F product: carboxypeptidase *F match_to: TR E1247396 e-52 W *F match_to: SP P42660 e-37 O (mosquito) *F match_to: GB L46594 e-32 O (mosquito) *F match_to: GB AA117009 e-26 M (Mus EST) *F match_to: GB AA118637 e-26 M (Mus EST) *F match_to: TR O04084 e-17 A *F match_to: SP P32826 e-26 A *F match_to: SP P38109 e-19 Y *F match_to: SP P09620 e-15 Y *F match_to: SP P10619 e-13 H *F match_to: TR E1296581 e-13 H *F EST: *F P1: DS00365 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR product from GH(2) *F and LP(2) libraries. *F ========================================================================== *F \+symbol: beat-B *F \+formal_name: BG:DS00365.4 *F name: beaten path-B *F UID: *F references: *F Teg Pipes to JR 29 May 1997 *F genefinder: *F genscan: 127(DS00365)/(30)(DS07486) *F match_to: TR Q94534 e-41 D(DS00365) *F EST: *F P1: DS00365, DS07486 *F direction: - *F SM_notes: Translation from alignment of cDNA sequence from T. Pipes on DS00365 *F (3';1148-2590) and DS07486 (5';17-1147). *F =========================================================================== *F \+symbol: BG:DS07486.3 *F \+formal_name: BG:DS07486.3 *F genefinder: *F genscan: 300 *F product: serine protease *F match_to: TR P91817 e-31 O (horseshoe crab) *F match_to: GB D87214 e-29 O (horseshoe crab) *F match_to: SP P26262 e-22 M *F match_to: SP P40313 e-20 H *F match_to: TR Q17800 e-14 W *F EST: LP01559.5' *F P1: DS07486 *F direction: + *F Nested_in: beat-B *F SM_notes: Translation from full-length LP01559. *F ========================================================================== *F \+symbol: BG:DS07486.4 *F \+formal_name: BG:DS07486.4 *F genefinder: *F genscan: 70 *F product: *F match_to: *F EST: *F P1: DS07486 *F direction: + *F Nested_in: beat-B *F SM_notes: Translation from Genscan. Kerrie reports PCR products from GH(3) *F and LP(3) libraries. *F ========================================================================== *F \+symbol: BG:DS07486.5 *F \+formal_name: BG:DS07486.5 *F genefinder: *F genscan: 63 *F product: *F match_to: *F EST: *F P1: DS07486 *F direction: + *F Nested_in: beat-B *F SM_notes: Translation from edited Genscan (took out 2 exons--low *F probabilities). Kerrie reports PCR products from GH(3) and LP(3) libraries. *F =========================================================================== *F \+symbol: BG:DS07486.2 *F \+formal_name: BG:DS07486.2 *F genefinder: 24 *F genscan: 54 *F product: outer arm dynein light chain 2 *F match_to: TR D1025094 e-34 O (sea urchin) *F match_to: GB AA923426 e-12 H (Hum EST) *F match_to: YPD 486343 e-8 Y *F EST: *F P1: DS07486 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F symbol: fs(2)35Ec *F phenotypic_class: female_sterile *F JR_notes: No alleles; overlapping deletion phenotype. *F =========================================================================== *F symbol: ms(2)35Eb *F name: male sterile (2) 35Eb *F UID: FBgn0004075 *F references: *F FBrf0082309 == Mahone et al., 1995, EMBO J. 14(9): 2043--2055 *F cytology: 35E1--35E1 *F cytology_data: Left limit from inclusion within Df(2L)RA5 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)Sco7 (FBrf0051973) *F phenotypic_class: male_sterile. *F JR_notes: No alleles; overlapping deletion phenotype. *F =========================================================================== *F symbol: fs(2)35Ed *F phenotypic_class: female_sterile *F JR_notes: No alleles; overlapping deletion phenotype. *F =========================================================================== *F \+symbol: beat-C *F \+formal_name: BG:DS00913.1 *F name: beaten path-C *F UID: *F references: *F Teg Pipes to JR 29 May 1997 *F genefinder: 26(DS00913) *F genscan: 157(DS00913) *F product: *F match_to: TR Q94534 e-169 D *F EST: *F P1: DS08681, DS00913 *F direction: + *F MA_notes: TE35D-GW19/RM5 is viable; hence beat-C is not lethal; = fs(2)35Ed ?. *F SM_notes: Translation from alignment of cDNA sequence from T. Pipes on *F DS08681 (5';10-791) and on DS00913 (3';718-4031). *F =========================================================================== *F \+symbol: BicC *F \+formal_name: BG:DS00913.2 *F name: Bicaudal C *F UID: FBgn0000182 *F references: *F FBrf0082309 == Mahone et al., 1995, EMBO J. 14(9): 2043--2055 *F FBrf0079309 == Saffman et al., 1995, A. Conf. Dros. Res. 36: 128B *F FBrf0087607 == Micklem, 1995, Dev. Biol. 172(2): 377--395 *F cytology: 35E1--35E1 *F cytology_data: Left limit from non-inclusion within Df(2L)Sco7 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)fn26 (FBrf0051973) *F genefinder: 130 *F genscan: 270(10 spans) *F product: RNA-binding-protein *F match_to: TR Q21593 e-12 W *F EST: LD07303.5'; LD20765.5'; LD08973.5'; CK01824.3'; LD17549.5'; *F LD22511.5'; LD22888.5' *F P1: DS00913 *F direction: + *F motifs: KH domain protein. *F homology: species == Homo sapiens; gene == IMAGE: 25351; EMBL: R12003; DRES: 46 *F genomic_sequence:U15928; g1932822 *F pr_sequence_TREMBL:Q24009 *F pr_sequence_PIR:S55051 *F ems_allele: *F BicC1 *F BicC2 *F BicC3 *F BicC4 *F BicC5 *F BicCAA29 *F BicCAA4 *F BicCAB74 *F BicCAB79 *F BicCAR108 *F BicCAR72 *F BicCAR96 *F BicCPE37 *F BicCPX1 *F BicCQL53 *F aberration_associated_allele: *F BicCSco-rv18 (Df(2L)Scorv18) *F BicCTE35D-19 (Df(2L)TE35D-19) *F phenotypic_class: female_sterile. *F SM_notes: Translation from alignment of EMBL U15928 (curated by MA). *F JR_notes: Sco-rv18 is beat- according to Fambrough, therefore not BicC *F allele. *F =========================================================================== *F \+symbol: beat *F \+formal_name: BG:DS00913.3 *F name: beaten path *F synonym: tric *F UID: FBgn0013433 *F references: *F FBrf0057894 == van Vactor et al., 1993, Cell 73(6): 1137--1153 *F FBrf0091058 == Fambrough and Goodman, 1996, Cell 87(6): 1049--1058 *F FBrf0074804 == Wilson, 1993, Curr. Biol. 3: 536--539 *F FBrf0092815 == Tessier-Lavigne and Goodman, 1996, Science 274(5290): *F 1123--1133 *F FBrf0101922 == Holmes et al., 1998, Genetics 148: 1189--1201 *F cytology: 35E1--35E1 *F cytology_data: Left limit from inclusion within Df(2L)RM5 (FBrf0091058) *F cytology_data: Right limit from molecular mapping relative to BicC *F (FBrf0091058) *F genefinder: (16) *F genscan: 106 *F product: *F match_to: *F EST: *F P1: DS00913 *F direction: + *F cDNA_sequence:U67057; g1519540 *F pr_sequence_TREMBL:Q94534 *F ems_allele: *F beat1 *F beat2 *F beat3 *F beattric *F aberration_associated_allele: *F In(2L)C163.41 *F In(2L)dppd36 *F phenotypic_class: vital. *F MA_notes: email from Heilig says that tric=beat (see email: From *F heilig@samiam.colorado.edu Wed Apr 29 18:57:23 1998) *F SM_notes: Translation from alignment of GenBank U67057. *F =========================================================================== *F \+symbol: BG:DS04095.1 *F \+formal_name: BG:DS04095.1 *F genefinder: (10) *F genscan: 64 *F product: *F match_to: *F EST: GH09478.5' *F P1: DS04095 *F direction: + *F SM_notes: Translation from full-length GH09478 (different from Genscan at 3'). *F =========================================================================== *F \+symbol: BG:DS04095.2 *F \+formal_name: BG:DS04095.2 *F genefinder: (15) *F genscan: 53 *F product: *F match_to: TR O16053 e-7 D (D.yakuba) *F EST: *F P1: DS04095 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: BG:DS04095.3 *F \+formal_name: BG:DS04095.3 *F genefinder: *F genscan: *F product: *F match_to: *F EST: GH12941.3' *F P1: DS04095 *F direction: + *F SM_notes: Translation from Genscan. *F =========================================================================== *F \+symbol: Ca-&agr;1D *F \+formal_name: BG:DS02795.1 *F name: Ca2+-channel protein &agr;1 subunit D *F synonym: l(2)35Fa *F UID: FBgn0001991 *F references: *F FBrf0080516 == Zheng et al., 1995, J. Neurosci. 15(2): 1132--1143 *F FBrf0082309 == Mahone et al., 1995, EMBO J. 14(9): 2043--2055 *F cytology: 35F1--35F1 *F cytology_data: Left limit from sequence databank entry U00690 *F cytology_data: Right limit from inclusion within Tp(2;2)A446 (citation *F unavailable) *F genefinder: 74/37/56/(18) *F genscan: 626/(38) *F product: Ca2+-channel-protein-&agr;1-subunit *F match_to: TR Q18698 0 W *F match_to: SP P24827 e-7 B *F match_to: TR Q13931 0 H *F match_to: TR Q01815 0 M *F match_to: SP P50077 e-12 Y *F EST: GH02029.5'; GH02129.5'; HL01607.5' *F P1: DS02795 *F direction: + *F cDNA_sequence:U00690; g457927 *F pr_sequence_TREMBL:Q24270 *F ems_allele: *F Ca-&agr;1DAR66 *F Ca-&agr;1DX10 *F Ca-&agr;1DX7 *F misc_allele: *F Ca-&agr;1DIK2 *F phenotypic_class: vital. *F SM_notes: Translation from alignment of EMBL U00690 (curated). Many other *F Ca channels from other species showed high homology, *F but these are just a random selection. *F =========================================================================== *F \+symbol: BG:DS02795.3 *F \+formal_name: BG:DS02795.3 *F genefinder: *F genscan: 108(DS02795)/63(DS07473) *F product: *F match_to: *F EST: *F P1: DS02795, DS07473 *F direction: + *F SM_notes: Translation from 3' edited Genscan on DS02795. Kerrie reports *F PCR products from GH(1) and LP(2) libraries. *F =========================================================================== *F \+symbol: BG:DS07473.1 *F \+formal_name: BG:DS07473.1 *F genefinder: 104 *F genscan: 506 *F product: calcium channel *F match_to: TR O08536 e-62 M *F match_to: TR O08533 e-62 M *F match_to: TR O08535 e-62 M *F match_to: TR O08534 e-62 M *F match_to: TR O08532 e-62 M *F match_to: SP P54289 e-62 H *F match_to: SP P34374 e-48 W *F match_to: YPD 1015903 e-22 Y *F EST: *F P1: DS07473 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR products from LD *F A8-F8(3) library. *F ========================================================================== *F \+symbol: PRL-1 *F \+formal_name: BG:DS07473.3 *F name: putative prenylated protein tyrosine phosphatase *F UID: FBgn0024734 *F genefinder: (14) *F genscan: (39) *F product: protein tyrosine phosphatase (prenylated) *F match_to: TR Q15259 e-23 H *F match_to: TR Q12974 e-23 H *F match_to: TR G2992630 e-23 M *F match_to: TR G3152650 e-31 W *F match_to: TR Q22582 e-31 W *F match_to: GB U42846 e-11 W *F EST: LD12894.3'; LD12894.5'; CK00486.5'; GM07690.5'; GM14563.5'; *F GM14708.5'; GM14720.5'; LD18273.5'; LD20164.5'; GH03107.5'; LD33945.5'; *F LD33967.5'; LD33906.5'; LD34413.5' *F P1: DS07473 *F direction: - *F cDNA_sequence:AF063902; g3135664 *F enzyme: protein-tyrosine-phosphatase (prenylated) == EC 3.1.3.48 *F P_element_allele: *F l(2)PZ03264 sequenced *F k09834 sequenced *F EP(2)0311 sequenced *F EP(2)2048 sequenced *F phenotypic_class: not vital *F phenotypic_notes: wing phenotype over deletions (Df(2L)el18, Df(2L)RN2). *F MA_notes: l(2)03264 has two inserts; that associated with this gene in *F FlyBase (FBgn0010534) is the 27F insertion. *F JR_notes: all excisions of 03264 are viable. EP(2)0311 is female *F semi-sterile o *F ver certain deletions in the 35F vicinity. *F SM_notes: Translation from full-length LD12894. *F LD12894 is significantly longer at the 5' end (a little longer at 3' end too) *F than AF063902 in GenBank. PZ03264 maps at 3235, EP(2)0311 maps at 8524, *F EP(2)2048 at 8597, k09834 at 8497. PRL-1 exon 1 is 2857 to 3346, exon 2 *F is 10 *F 264 to 10341. *F ========================================================================== *F \+symbol: BG:DS07473.2 *F \+formal_name: BG:DS07473.2 *F genefinder: 48 *F genscan: 146 *F product: *F match_to: *F EST: LD25338.5'; LD29644.5' *F P1: DS07473, (DS02740) *F direction: + *F SM_notes: Translation from full-length LD25338. LD25338 full length sequence *F only 1-1323 of 3159 bp aligns to DS07473, and the 3' end is not on any *F other P1 *F (DS02740, DS09218, or DS02780). Could be a very, very long intron or *F chimeric clone. *F =========================================================================== *F \+symbol: twe *F \+formal_name: BG:DS02740.1 *F name: twine *F synonym:l(2)35Fh *F UID: FBgn0002673 *F UID: FBgn0016862 *F references: *F FBrf0055587 == Alphey et al., 1992, Cell 69: 977--988 *F FBrf0074608 == Thomas et al., 1994, Cell 77(7): 1003--1014 *F FBrf0073190 == Gonczy et al., 1994, Cell 77(7): 1015--1025 *F FBrf0055870 == Courtot et al., 1992, Development 116(2): 405--416 *F cytology: 35F1--35F7 *F cytology_data: Left limit from inclusion within Df(2L)RN2 (FBrf0055870) *F cytology_data: Right limit from non-inclusion within Df(2L)II30 (citation *F unavailable) *F enzyme: protein-tyrosine-phosphatase == EC 3.1.3.48 *F genefinder: 45 *F genscan: 123 *F product: protein-tyrosine-phosphatase *F match_to: SP Q29029 e-40 O (pig) *F match_to: SP P30307 e-39 H *F match_to: SP P48967 e-37 M *F match_to: SP P30606 e-33 M *F match_to: SP P30634 e-21 W *F match_to: SP P23748 e-18 Y *F EST: LD04211.5'; LD02913.5'; LD10764.5'; LD16907.5; LD19349.5'; LD19391.5'; *F LD20092.5'; LD23305.5' *F P1: DS07473, DS02740 *F direction: - *F homology: species == Schizosaccharomyces pombe; gene == cdc25 *F cDNA_sequence:M94158; g157056 *F cDNA_sequence:X69018; g8756 *F pr_sequence_SwissProt:Q03019 *F pr_sequence_PIR:A41910 *F pr_sequence_PIR:S26692 *F misc_allele: *F twe1 *F P_element_allele: *F k08310 *F EP(2)0613 sequenced *F aberration_associated_allele: *F tweDTD22 (T(2;4)DTD22) *F Df(2L)RN2 broken within twe *F phenotypic_class: maternal_effect_lethal; male_sterile. *F SM_notes: Translation from alignment of GenBank M94158. *F JR_notes: k08310 is female fertile. Synthetic deletion T(2;4)GV101 DTD22 *F is lethal with RN2. *F Both RN2 and DTD22 break in twe (RN2 has been molecularly mapped, DTD22 is *F mutant for twe), so the new synthetic and RN2 should only overlap for *F twe, therefore twe = 35Fh. *F =========================================================================== *F \+symbol: BG:DS02740.2 *F \+formal_name: BG:DS02740.2 *F synonym: MET30 *F genefinder: 20 *F genscan: 60 *F product: *F match_to: TR Q19986 e-43 W *F match_to: GB AA008890 e-15 M (Mus EST) *F match_to: GB AI147754 e-15 H (Hum EST) *F EST: *F P1: DS02740 *F direction: + *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F =========================================================================== *F \+symbol: crp *F \+formal_name: BG:DS02740.3 *F name: cropped *F synonym: lethal (2) 35Fd *F UID: FBgn0001994 *F references: *F FBrf0092259 == Chiu and Krasnow, 1997, A. Conf. Dros. Res. 38: 229A *F cytology: 35F1--35F2 *F cytology_data: Left limit from inclusion within Df(2L)RN2 (FBrf0051973) *F cytology_data: Right limit from inclusion within Df(2L)TE35D-13 (FBrf0092813) *F genefinder: (11)/94 *F genscan: 223 *F product: AP-4 *F match_to: SP Q01664 e-16 H *F match_to: SP P34474 e-11 W *F match_to: SP P26687 e-11 M *F EST: LD12417.3'; LD12417.5'; LD02349.5'; LD02353.5'; GM01378.5'; *F GM03832.5'; GM08317.5'; GM08864.5'; LD04473.5'; LD05189.5'; LD07249.5'; *F LD11116.5'; LD18457.5'; LD21475.5'; LD26263.5'; LD28118.5'; LD34528.5'; *F LD27601.5' *F P1: DS02740 *F direction: - *F ems_allele: *F l(2)35FdRAR46 *F P_element_allele: *F l(2)35Fd00232 sequenced *F l(2)35Fd03101 *F l(2)35Fd06872 *F l(2)35Fdk00809 *F k05211 *F l(2)35Fdk05519 cluster: k05527 *F l(2)35Fdk05601 cluster: *F k05635;k05648;k05608;k05616;k05625;k05640;k05642;k05649 *F l(2)35Fdk05814 *F l(2)35Fdk06207 *F l(2)35Fdk07829 sequenced *F l(2)35Fdk08007 *F l(2)35Fdk08806 *F l(2)35Fdk09214 cluster: k09225;k09232 *F l(2)35Fdk09704 *F l(2)35Fdk10415 *F l(2)35Fdk11024 *F l(2)35Fdk13611 *F l(2)35Fdk16129 *F l(2)35Fdk21602 *F EP(2)0721 sequenced *F EP(2)0814 sequenced in Su(Ste) sequence *F k03505 *F misc_allele: *F l(2)35Fd2 *F phenotypic_class: vital. *F phenotypic_notes: Required for tracheal branching (FBrf0092259); pupal *F lethal (k11024, BDGP); early-larval lethal (k09232, k05519, BDGP). *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F =========================================================================== *F \+symbol: BG:DS02740.4 *F \+formal_name: BG:DS02740.4 *F genefinder: 70 *F genscan: 236 *F product: un-1 (protein kinase A anchoring protein) *F match_to: TR G2707344 e-35 H *F match_to: TR Q10955 e-11 W *F EST: HL02329.5' *F P1: DS02740 *F direction: + *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F ========================================================================== *F \+symbol: BG:DS02740.5 *F \+formal_name: BG:DS02740.5 *F genefinder: 23 *F genscan: (33) *F product: *F match_to: *F EST: *F P1: DS02740 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR products from *F GM(1), GH(1), and LP(2) libraries. *F ========================================================================== *F \+symbol: l(2)35Fb *F \+formal_name: BG:DS02740.6 *F name: lethal (2) 35Fb *F UID: FBgn0001992 *F references: *F genefinder: 53 *F genscan: 142 *F product: cytochrome P450 *F match_to: GB U44753 e-41 D (Cyp18) *F match_to: SP Q95078 e-40 D (Cyp18) *F match_to: SP P00178 e-56 O (rabbit) *F match_to: SP P20813 e-51 H *F match_to: TR G2920650 e-51 M *F match_to: TR G2736526 e-42 W *F match_to: TR D1029479 e-25 A *F EST: *F P1: DS02740 *F direction: + *F cytology: 35F1--35F12 *F cytology_data: Left limit from inclusion within Df(2L)II30 (FBrf0092813) *F cytology_data: Right limit from inclusion within Df(2L)II30 (FBrf0092813) *F spontaneous_allele *F l(2)35Fbck *F ems_allele: *F l(2)35FbAS96 *F phenotypic_class: vital; escapers disorientated (Willingham). *F SM_notes: Translation from Genscan. Cyp2b homolog; no longer have any cDNA *F sequence (Ling & Damon couldn't find). *F =========================================================================== *F \+symbol: heix *F \+formal_name: BG:DS02740.7 *F name: heixuedian *F synonym: lethal (2) 35Fc *F synonym: l(2)35Fc *F synonym: ipa-6d *F UID: FBgn0001993 *F references: *F cytology: 35F6--35F7 *F cytology_data: Left limit from in situ hybridisation (FBrf0067338) *F cytology_data: Right limit from in situ hybridisation (FBrf0067338) *F genefinder: 43 *F genscan: 103 *F product: ipa-6d *F match_to: GB AA087043 e-41 M (Mus EST) *F match_to: GB W58989 e-15 M (Mus EST) *F match_to: GB AA306118 e-27 H (Hum EST) *F match_to: GB R52058 e-13 H (Hum EST) *F EST: LD08373.5'; CK02500.5'; LD06174.5'; LD08295.5'; LD09003.5'; *F LD10383.5'; LD13786.5'; LD14441.5'; LD14872.5'; LD15648.5'; LD16196.5'; *F LD18123.5'; LD18475.5'; *F P1: DS02740 *F direction: - *F ems_allele: *F l(2)35Fc1 *F P_element_allele: *F l(2)35Fck11403 sequenced *F l(2)35Fck12401 sequenced *F l(2)k07114 *F misc_allele: *F l(2)35Fc91k *F phenotypic_class: vital. *F phenotypic_notes: pupal lethal (k07114, BDGP). *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F =========================================================================== *F \+symbol: BG:DS02740.8 *F \+formal_name: BG:DS02740.8 *F genefinder: (11) *F genscan: 70 *F product: mkr2 (zinc finger) *F match_to: TR E1308763 e-26 D (Kr-H) *F match_to: TR E1308765 e-26 D (Kr-H) *F match_to: SP P51523 e-31 H *F match_to: TR Q92951 e-32 H *F match_to: SP Q13360 e-32 H *F match_to: TR Q62512 e-32 M *F match_to: SP P15620 e-29 M *F match_to: TR Q22874 e-23 W *F match_to: TR O02265 e-23 W *F match_to: SP P47043 e-18 Y *F EST: *F P1: DS02740 *F direction: + *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey (5' and 3' ends) and Genscan in middle. *F ========================================================================== *F \+symbol: BG:DS02740.9 *F \+formal_name: BG:DS02740.9 *F genefinder: (15) *F genscan: *F product: glial maturation factor *F match_to: TR D1019982 e-22 H *F match_to: SP P17774 e-23 H *F match_to: SP Q63228 e-28 O (rat) *F match_to: GB W50254 e-20 M (Mus EST) *F EST: *F P1: DS02740 *F direction: - *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F ========================================================================== *F \+symbol: BG:DS02740.10 *F \+formal_name: BG:DS02740.10 *F genefinder: *F genscan: 54 *F product: un-4 *F match_to: *F EST: GM03132.5'; GM03132.3'; CK01361.c *F P1: DS02740 *F direction: + *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey and GM03125.5and3 at 5' and 3' ends (extend longer). *F ========================================================================== *F \+symbol: anon-35Fa *F \+formal_name: BG:DS02740.11 *F synonym: ZK4118 *F UID: FBgn0015338 *F references: *F FBrf0082538 == Roth et al., 1995, Cell 81(6): 967--978 *F cytology: 35F6--35F12 *F cytology_data: Left limit from molecular mapping relative to cni (FBrf0082538) *F cytology_data: Right limit from inclusion within Df(2L)III18 (FBrf0082538) *F genefinder: 26 *F genscan: (36) *F product: ZK4118 *F match_to: TR E1318685 e-31 H *F match_to: GB AA139591 e-28 M (Mus EST) *F match_to: GB AA485358 e-14 H (Hum EST) *F match_to: TR Q23483 e-12 W *F EST: GM07085.5'; GM13502.5'; LD33770.5' *F P1: DS02740 *F direction: - *F FB_internal_note: Encodes 1kb transcript III in Fig 6A pg 973. *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F =========================================================================== *F \+symbol: Sed5 *F \+formal_name: BG:DS02740.12 *F synonym: lethal (2) 35Ff *F synonym: = anon-35Fb *F UID: FBgn0001996 *F UID: FBgn0015339 *F UID: FBgn0011708 *F references: *F FBrf0081366 == Banfield et al., 1994, J. Cell Biol. 127(2): 357--371 *F FBrf0082538 == Roth et al., 1995, Cell 81(6): 967--978 *F cytology: 35F1--35F12 *F cytology_data: Left limit from inclusion within Df(2L)RN2 (FBrf0051973) *F cytology_data: Right limit from non-inclusion within Df(2L)III18 (citation *F unavailable) *F genefinder: 53 *F genscan: 117 *F product: vesicle-targeting-protein *F match_to: SP Q13190 e-71 H *F match_to: SP Q20797 e-46 W *F match_to: GB AA072141 e-31 M (Mus EST) *F match_to: SP Q01590 e-22 Y *F match_to: TR G2981439 e-19 A *F EST: LD02623.3'; LD02623.5'; GM06766.5'; LD28045.5'; LD29042.5' *F P1: DS02740 *F direction: + *F homology: species == Saccharomyces cerevisiae; gene == SED5; SGDID: L0001861 *F cDNA_sequence:X78219; g467559 *F pr_sequence_TREMBL:Q24509 *F pr_sequence_PIR:S43101 *F ems_allele: *F l(2)35FfAR113 *F phenotypic_class: vital. *F FB_internal_note: Encodes 1.7kb transcript II in Fig 6A pg 973. *F SM_notes: Translation from alignment of GenBank X78219. *F =========================================================================== *F \+symbol: cni *F \+formal_name: BG:DS02740.13 *F name: cornichon *F UID: FBgn0000339 *F references: *F FBrf0082538 == Roth et al., 1995, Cell 81(6): 967--978 *F cytology: 35F6--35F12 *F cytology_data: Left limit from inclusion within Df(2L)III18 (FBrf0082538) *F cytology_data: Right limit from inclusion within Df(2L)II30 (FBrf0092813) *F homology: species == Homo sapiens; gene == IMAGE: 51056; EMBL: H18748; DRES: 82 *F genefinder: (11) *F genscan: *F product: *F match_to: TR Q22361 e-21 W *F match_to: GB Z78065 e-10 W *F match_to: TR O35372 e-29 M *F match_to: GB AA049525 e-28 M (Mus EST) *F match_to: GB AA309041 e-20 H (Hum EST) *F match_to: GB H18748 e-17 H (Hum EST) *F EST: *F P1: DS02740 *F direction: - *F cDNA_sequence:U28069; g886769 *F pr_sequence_PIR:A56724 *F pr_sequence_SwissProt:P49858 *F ems_allele: *F cni1 *F cniAA12 aka AA112 ? *F cniCF5 *F phenotypic_class: maternal_effect_lethal. *F SM_notes: Translation from alignment of GenBank U28069. *F =========================================================================== *F \+symbol: fzy *F \+formal_name: BG:DS02740.14 *F name: fizzy *F UID: FBgn0001086 *F references: *F FBrf0081900 == Dawson et al., 1995, J. Cell Biol. 129(3): 725--737 *F FBrf0058082 == Dawson et al., 1993, Development 117(1): 359--376 *F FBrf0084373 == Sigrist et al., 1995, EMBO J. 14(19): 4827--4838 *F FBrf0085992 == Stratmann and Lehner, 1996, Cell 84(1): 25--35 *F cytology: 35F6--35F12 *F cytology_data: Left limit from inclusion within Df(2L)III18 (FBrf0082538) *F cytology_data: Right limit from inclusion within Df(2L)II30 (FBrf0092813) *F homology: species == Saccharomyces cerevisiae; gene == cdc20; SGDID: L0000259 *F genefinder: 65 *F genscan: 114 *F product: *F match_to: TR Q12834 e-120 H *F match_to: TR G3088632 e-119 O (rat) *F match_to: GB AA008043 e-34 M (Mus EST) *F match_to: TR E1310059 e-83 A *F match_to: TR Q09649 e-71 W *F match_to: SP P53197 e-65 Y *F match_to: SP P26309 e-55 Y *F EST: LD08545.5'; CK02051.3'; GM02166.5'; GM10572.5'; LD08754.5'; *F LD11224.5'; LD12520.5'; LD13309.5'; LD14034.5'; LD18042.5'; LD18190.5'; *F LD22440.5'; LD22633.5'; LD22763.5'; LD23923.5'; LD24209.5'; LD24520.5'; *F LD24735.5'; LD33694.5'; LD26949.5'; LD30572.5'; LD31127.5'; GH02903.5'; *F LD32995.5'; LD34516.5' *F P1: DS02740 *F direction: + *F cDNA_sequence:U22419; g1109772 *F pr_sequence_TREMBL:Q24044 *F ems_allele: *F fzy1 *F fzy2 *F fzy3 *F X_ray_allele: *F fzyID *F misc_allele: *F fzy4 *F fzy5 *F fzy6 *F fzy7 *F fzy8 *F P_element_allele: *F EP(2)1028 sequenced *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank U22419. *F =========================================================================== *F \+symbol: cact *F \+formal_name: BG:DS02740.15 *F name: cactus *F UID: FBgn0000250 *F references: *F FBrf0053786 == Roth et al., 1991, Development 112: 371--388 *F FBrf0055573 == Geisler et al., 1992, Cell 71: 613--621 *F FBrf0055575 == Kidd, 1992, Cell 71: 623--635 *F cytology: 35F6--35F12 *F cytology_data: Left limit from inclusion within Df(2L)III18 (FBrf0082538) *F cytology_data: Right limit from inclusion within Df(2L)II30 (FBrf0092813) *F motifs: Ankyrin-repeat protein. *F genefinder: 51/(20) *F genscan: 143/(33) *F product: *F match_to: TR Q08353 e-11 O (pig) *F EST: LD10835.5'; CK01266.5'; CK01266.5'; HL04371.5'; HL04374.3'; *F LD03018.5'; LD03773.5'; LD05260.5'; LD08581.5'; LD10168.5'; *F LD10910.5'; LD13068.5'; LD14407.5'; LD14691.5'; LD15008.5'; *F LD16234.5'; LD17086.5'; LD19013.5'; LD19965.5'; LD21118.5'; *F LD23729.5'; LD24255.5'; LD26362.5'; LD30959.5'; LD30371.5'; LD33294.5'; *F LD33754.5'; LD33069.5'; LD31391.5'; LD34227.5'; LD28651.5'; LD29203.5' *F P1: DS02740 *F direction: - *F homology: species == Saccharomyces cerevisiae; gene == SWI6; SGDID: L0002254 *F homology: species == Homo sapiens; gene == NFKBI; GDB: 131399; OMIM: 164008 *F genomic_sequence:L03369 *F genomic_sequence:L03367; g157036 *F genomic_sequence:L03368; g157038 *F cDNA_sequence:L04964; g157041 *F pr_sequence_SwissProt:Q03017 *F pr_sequence_PIR:A44269 *F pr_sequence_PIR:A44268 *F pr_sequence_PIR:B44268 *F ems_allele: *F cact1 *F cact2 *F cact3 *F cact4 *F cact5 *F cact6 *F cact7 *F cact8 *F cact9 *F cact10 *F cact11 *F cact12 *F cact13 *F cact14 *F cact15 *F cact16 *F cact17 *F cact18 *F cactAA51 *F cactAA58 *F cactAB10 *F cactAB13 *F cactSu *F cactX6 *F cactY11 *F cactRN48 *F P_element_allele: *F cact255 *F k17027 sequenced *F k17003 sequenced *F PM_dysgenesis_allele: *F cact19 *F cact20 *F cact21 *F cact22 *F cactBQ *F cactE10 *F cactE13 *F cactE15 *F cactE17 *F cactE19 *F cactE2 *F cactE20 *F cactE25 *F cactE29 *F cactE4 *F cactE6 *F cactE8 *F cactEP *F cactP1 *F cactP3 *F cactP5 *F cactP6 *F cactP7 *F X_ray_allele: *F cactE10R01 *F cactE10RJ1 *F misc_allele: *F cact99 *F aberration_associated_allele: *F cactchif64 (Df(2L)cact-255rv64) *F Df(2L)H60-3 *F Df(2L)II30 *F Df(2L)III18 *F phenotypic_class: vital. *F SM_notes: Translation from alignment of GenBank L04964. *F k17027 & k17003 (66739) may be alleles of cact (66479-53838) since 260bp *F upstream. *F =========================================================================== *F \+symbol: anon-35F/36A *F \+formal_name: BG:DS02740.16 *F synonym: un5 *F UID: FBgn0014092 *F references: *F FBrf0055573 == Geisler et al., 1992, Cell 71: 613--621 *F cytology: 35F6--35F12 *F cytology_data: Left limit from molecular mapping relative to cni (FBrf0082538) *F cytology_data: Right limit from molecular mapping relative to cact *F (FBrf0055573) *F genefinder: 25 *F genscan: 50 *F product: un5 (NGG-1 interacting factor 3, NIF3) *F match_to: SP P53081 e-31 Y *F match_to: GB W63821 e-25 M (Mus EST) *F match_to: GB AA096836 e-25 M (Mus EST) *F match_to: GB H15210 e-15 H (Hum EST) *F match_to: GB H84119 e-15 H (Hum EST) *F EST: LD28566.5'; CK01378.3' *F P1: DS02740 *F direction: + *F JR_notes: John Tower calls this P2A; between 1kb and 2kb from 5' end of cact. *F Deletion excisions of k17027 are either cact- or 35Fe- (as predicted). *F Neither are 35Fg- i.e. DS02740.16 is not 35Fg. *F SM_notes: k17027 & k17003 (66739) may be alleles of this (67924-71177) but *F far (1185bp away). *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F =========================================================================== *F \+symbol: l(2)35Fe *F \+formal_name: BG:DS02740.17 *F name: lethal (2) 35Fe *F synonym: Rpl4 *F UID: FBgn0001995 *F references: *F cytology: 35F11--35F12 *F cytology_data: Left limit from in situ hybridisation (FBrf0067338) *F cytology_data: Right limit from in situ hybridisation (FBrf0067338) *F genefinder: 35 *F genscan: (42) *F product: RPl4 (50S ribosomal protein L4) *F match_to: TR O17005 e-47 W *F match_to: SP P38516 e-23 B (50S ribosomal protein L4) *F match_to: GB AA045589 e-21 H (Hum EST) *F match_to: GB AA307866 e-28 H (Hum EST) *F match_to: GB AA124364 e-31 M (Mus EST) *F match_to: TR E1240162 e-8 A *F match_to: TR E1240160 e-8 A *F EST: LD33485.5' *F P1: DS02740 *F direction: - *F ems_allele: *F l(2)35Fe1 *F P_element_allele: *F l(2)35Fek14608 sequenced *F phenotypic_class: vital; mitotic; polyploid cells Gatti. *F SM_notes: Translation from alignment of partial(?) cDNA sequence from Ling *F Hong *F & Damon Harvey. *F ========================================================================== *F \+symbol: BG:DS02740.18 *F \+formal_name: BG:DS02740.18 *F genefinder: *F genscan: 336 *F product: *F match_to: *F EST: *F P1: DS02740 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports no PCR products after 2 *F attempts. *F ========================================================================== *F \+symbol: BG:DS02740.19 *F \+formal_name: BG:DS02740.19 *F genefinder: *F genscan: 205 *F product: *F match_to: *F EST: *F P1: DS02740 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR products from GH(4) *F and LP(2) libraries. *F =========================================================================== *F \+symbol: BG:DS09218.1 *F \+formal_name: BG:DS09218.1 *F genefinder: 23 *F genscan: 52 *F product: *F match_to: *F EST: *F P1: DS09218 *F direction: - *F SM_notes: Translation from Genscan. Kerrie reports PCR products from LD *F A8-F8 library(6). *F ========================================================================== *F \+symbol: chif *F \+formal_name: BG:DS09218.2 *F name: chiffon *F UID: FBgn0000307 *F references: *F FBrf0078863 == Landis and Tower, 1995, A. Conf. Dros. Res. 36: 118A *F FBrf0054123 == Schupbach and Wieschaus, 1991, Genetics 129: 1119--1136 *F cytology: 35F6--36A2 *F cytology_data: Left limit from inclusion within Df(2L)cact-255rv64 *F (FBrf0058564) *F cytology_data: Right limit from inclusion within Df(2L)RA5 (FBrf0051973) *F genefinder: 44 *F genscan: 570 *F product: *F match_to: TR E1288127 e-7 O (S. pombe) *F EST: LD14373.3'; LD14373.5'; LD19808.5'; LD19808.3'; GM06264.5'; *F GM12481.5'; HL02831.5'; GH01539.3'; LD28038.5' *F P1: DS09218 *F direction: - *F ems_allele: *F chif1 *F chif2 *F chif3 *F chif4 *F chifDB23 *F chifPS55 *F chifQY42 *F P_element_allele: *F chifA507 *F n(2)k04216 sequenced *F phenotypic_class: female_sterile. *F JR_notes: k04216 is viable and fertile with chif. *F SM_notes: Translation from alignment of cDNA from G. Landis and J. Tower. *F k04216 inserted at 10147 of DS09218, LD19808.5' at 10154-9758, so P inserted *F in first exon 7bp downstream of EST start. *F =========================================================================== *F \+symbol: BG:DS09218.3 *F \+formal_name: BG:DS09218.3 *F genefinder: 39 (1 span) *F genscan: *F product: *F match_to: *F EST: LD12474.3'; LD12474.5'; LD27171.5'; LD33101.5'; *F P1: DS09218 *F direction: + *F SM_notes: Translation from full-length LD12474 (no good ORF). *F =========================================================================== *F \+symbol: BG:DS09218.4 *F \+formal_name: BG:DS09218.4 *F genefinder: 86 *F genscan: 179 *F product: protein disulfide isomerase *F match_to: SP Q11067 e-112 W *F match_to: SP Q15084 e-108 H *F match_to: TR G2702281 e-70 A *F match_to: GB W80331 e-42 M (Mus EST) *F match_to: GB M76982 e-14 Y *F match_to: GB X52313 e-14 Y *F match_to: GB X54535 e-14 Y *F EST: LD05503.3'; LD05503.5'; GM02162.5'; GM05173.5'; GM12483.5'; *F LD12134.5'; LD14462.5'; LD14683.5'; LD20301.5'; LD23109.5'; LD24243.5'; *F LD24557.5'; LD33982.5'; LD29677.5'; LD31351.5'; LD31057.5'; LD31108.5'; *F LD25852.5'; LD28038.5' *F P1: DS09218 *F direction: - *F SM_notes: Translation from full-length LD05503. *F =========================================================================== *F \+symbol: BG:DS09218.5 *F \+formal_name: BG:DS09218.5 *F genefinder: 22 *F genscan: 78 *F product: *F match_to: TR Q24622 e-12 O (D.pseudoobscura Toll) *F EST: *F P1: DS09218 *F direction: + *F SM_notes: Translation from Genscan. Kerrie reports PCR product from LP(1) *F library. *F ========================================================================== *F \+symbol: BG:DS02780.1 *F \+formal_name: BG:DS02780.1 *F genefinder: 38 *F genscan: 125 *F product: *F match_to: GB L25390 e-12 O (Dros. pseudoobscura Tl) *F EST: CK01518.3'; CK01518.5'; GH08155.5' *F P1: DS02780 *F direction: - *F SM_notes: Translation from full-length CK01518. *F =========================================================================== *F \+symbol: Idgf1 *F \+formal_name: BG:DS02780.5 *F genefinder: 31 *F genscan: 81 *F product: imaginal disc growth factor *F match_to: TR Q23997 e-121 D *F match_to: TR O15993 e-28 O (Penaeus japonicus) *F match_to: TR Q13231 e-25 H *F match_to: SP P36222 e-26 H *F match_to: TR Q15749 e-26 H *F match_to: SP Q62010 e-20 M *F match_to: TR Q61362 e-20 M *F match_to: SP Q11174 e-13 W *F match_to: LD25940.5' e-34; LD12729.5' e-34; LD22916.5' e-34; LD30495.5' *F e-34 etc. *F EST: GM09616.5'; GH07837.5'; GH03533.5'; GH07581.5'; GH04843.3' *F P1: DS02780 *F direction: + *F SM_notes: Translation from ESTs (5' & 3' ends) and Genscan. *F =========================================================================== *F \+symbol: Idgf2 *F \+formal_name: BG:DS02780.4 *F genefinder: 45 *F genscan: 101 *F product: imaginal disc growth factor *F match_to: TR Q23997 e-121 D *F match_to: TR O15993 e-28 O (Penaeus japonicus) *F match_to: TR Q13231 e-25 H *F match_to: SP P36222 e-26 H *F match_to: TR Q15749 e-26 H *F match_to: SP Q62010 e-20 M *F match_to: TR Q61362 e-20 M *F match_to: SP Q11174 e-13 W *F match_to: LD25940.5' e-34; LD12729.5' e-34; LD22916.5' e-34; LD30495.5' *F e-34 etc. *F EST: GH12581.5'; HL02712.5'; GH02092.5'; GH02192.5'; GH05540.5'; *F GH06632.5'; GH07990.5'; GH10214.5'; GH12262.5'; GH15212.5'; LP05630.3' *F P1: DS02780 *F direction: + *F SM_notes: Translation from full-length GH12581. *F =========================================================================== *F \+symbol: Idgf3 *F \+formal_name: BG:DS02780.2 *F genefinder: 45 *F genscan: 125 *F product: imaginal disc growth factor *F match_to: TR Q23997 e-121 D *F match_to: TR O15993 e-28 O (Penaeus japonicus) *F match_to: TR Q13231 e-25 H *F match_to: SP P36222 e-26 H *F match_to: TR Q15749 e-26 H *F match_to: SP Q62010 e-20 M *F match_to: TR Q61362 e-20 M *F match_to: LD25940.5' e-34; LD12729.5' e-34; LD22916.5' e-34; LD30495.5' *F e-34 etc. *F EST: CK00436.5'; CK00436.3'; GM05553.5'; GH07453.5'; GH09372.5'; GM04853.5' *F P1: DS02780 *F direction: + *F SM_notes: Translation from full-length CK00436. *F =========================================================================== *F symbol: l(2)35Fg *F UID: FBgn0015959 *F references: *F cytology: 35F6--36A2 *F cytology_data: Left limit from inclusion within Df(2L)cact-255rv64 *F (FBrf0067338) *F cytology_data: Right limit from inclusion within Df(2L)RA5 (FBrf0067338) *F P_element_allele: *F l(2)35Fgk08106 *F phenotypic_class: vital. *F JR_notes: Defined by l(2)k08106, but multiple insert (two inserts in 35F). *F =========================================================================== *F \+symbol: dac *F \+formal_name: BG:DS02780.3 *F name: dachshund *F synonym: l(2)36Ae *F UID: FBgn0005677 *F references: *F FBrf0075101 == Mardon et al., 1994, Development 120: 3473--3486 *F FBrf0087175 == Garrity et al., 1996, Cell 85(5): 639--650 *F FBrf0089691 == Huang and Kunes, 1996, Cell 86(3): 411--422 *F FBrf0091167 == Shen and Mardon, 1997, Development 124(1): 45--52 *F FBrf0092671 == Roush, 1997, Science 275(5300): 618--619 *F FBrf0092657 == Pignoni and Zipursky, 1997, Development 124(2): 271--278 *F cytology: 36A1--36A2 *F cytology_data: Left limit from in situ hybridisation (FBrf0067338) *F cytology_data: Right limit from in situ hybridisation (FBrf0067338) *F genefinder: 50 *F genscan: 123 *F product: *F match_to: *F EST: *F P1: DS02780 *F direction: - *F cDNA_sequence:U19269; g624298; g624297; g624300; g624296; g624299 *F pr_sequence_TREMBL:Q24027 *F pr_sequence_TREMBL:Q24028 *F pr_sequence_TREMBL:Q24029 *F pr_sequence_TREMBL:Q24030 *F pr_sequence_TREMBL:Q24031 *F misc_allele: *F dac2 *F dac3 *F dac5 *F dac6 *F dac7 *F dac8 *F dac9 *F P_element_allele: *F dacrK364 *F phenotypic_class: vital; escapers with crippled legs *F SM_notes: Only nucleotides 3037-4976 of gene. Translation from aligned *F GenBank sequence U19269 . *F =========================================================================== *F Genes whose status is uncertain, or have not been mapped though may be in *F region. *F =========================================================================== *F symbol: l(2)35Ab *F UID: FBgn0013532 *F references: *F FBrf0075330 == J. Roote, Personal communication to FlyBase, 16 November *F 1994 *F cytology: 35A3--35B1 *F cytology_data: Included in Df(2L)fn2 *F cytology_data: Not included in Df(2L)fn3 *F ems_allele: *F l(2)35Ab1 *F phenotypic_notes: GR17 lethality. *F status: uncertain. *F =========================================================================== *F symbol: snRNA:U5:35EF *F name: small nuclear RNA U5 at 35EF *F UID: FBgn0003936 *F references: *F FBrf0040068 == Saluz et al., 1983, Nucleic Acids Res. 11: 77--90 *F FBrf0048816 == Saluz et al., 1988, Nucleic Acids Res. 16: 3582 *F cytology: 35E1--35F12 *F cytology_data: Left limit from in situ hybridisation (FBrf0040068) *F cytology_data: Right limit from in situ hybridisation (FBrf0040068) *F product: snRNA-U5 *F =========================================================================== *F symbol: DopR *F name: Dopamine receptor *F synonym: Dop1 *F cytology: 35E--35F *F na_sequence:X77234; g479128 *F na_sequence:U22106; g722319 *F pr_sequence_SwissProt:P41596 *F pr_sequence_TREMBL:Q24038 *F pr_sequence_PIR:S68780 *F pr_sequence_PIR:S44275 *F MA_notes: No match with BDGP sequences of X77234 via their BLAST server. *F July 14 97.; Sima says may be DS05899.2.3 *F =========================================================================== *F symbol: Nc34CD *F name: Neural conserved at 34CD *F UID: FBgn0005690 *F references: *F FBrf0054219 == Kokoza et al., 1991, Genetika, Moscow 27: 51--60 *F FBrf0056302 == Perelygina et al., 1992, Genetika, Moscow 28(3): 98--104 *F cytology: 34C1--34D8 *F misc_notes: said to encode 2, 1.5 and 1.2-kb transcripts. *F status: uncertain. *F e=========================================================================== *F symbol: fs(2)lto1 *F UID: FBgn0005615 *F synonym: PN48 *F references: *F FBrf0049878 == Schupbach and Wieschaus, 1989, Genetics 121: 101--117 *F FBrf0054123 == Schupbach and Wieschaus, 1991, Genetics 129: 1119--1136 *F FBrf0089631 == Digilio et al., 1996, Dev. Biol. 178(1): 90--100 *F cytology: 35E6--36A7 *F ems_allele: *F fs(2)lto11 *F phenotypic_class: female_sterile. *F JR_notes: Fertile over A48, r10, osp29, cact225rv64 - the latter is Df *F 35F;36D *F =========================================================================== *F symbol: bls *F name: bractless *F cytology: 35E6--36A7 *F phenotypic_notes: Lacks bracts on bristles. *F JR_notes: Information from Lewis Held. *F =========================================================================== *F symbol: ifx *F references: *F Verheyen et al., 1996, Genetics 144(3): 1127--1141 *F \*c 34C1--34D4 *F JR_notes: probably distal to region *F JR_notes: semilethal over el88, complements b84h50 # *U FBrf0112155 *a Strigini *b M. *t 1999.10.27 *T personal communication to FlyBase *u *F From Maura.Strigini@embl-heidelberg.de Wed Oct 27 09:58:09 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 27 Oct 1999 09:58:09 +0100 *F X-Sender: strigini@popserver.embl-heidelberg.de *F Mime-Version: 1.0 *F Date: Wed, 27 Oct 1999 11:00:18 +0200 *F To: gm119@gen.cam.ac.uk *F From: Maura Strigini *F Subject: FlyBase query *F Dear Gillian, *F I got this message from Dirk Bohmann via Julia Zetlinger: *F >>Dear Dr. Bohmann, *F >> *F >>I am curating your paper for FlyBase: *F >> *F >>Zeitlinger and Bohmann, 1999, Development 126(17): 3947--3956 *F >> *F >>and I have a question about the GFP constructs you used. *F >> *F >>You used 2 constructs: *F >> *F >>UAS-GFP *F >>UAS-EGFP *F >> *F >> *F >>I would be grateful if you could tell me some more details about the *F >>UAS-EGFP construct, so that I can work out whether it corresponds to a *F >>UAS-GFP line we have already in FlyBase or whether it is new. *F >> *F >>Specifically, could you tell me: *F >> *F >>a. a reference where UAS-EGFP has been used. *F >>b. who you got the line from. *F >>c. presumably the 'EGFP' means that this is not the unmodified GFP - *F >>does EGFP have the S65T mutation, or some other mutation that enhances *F >>the fluorescence ? *F >> *F >>I look forward to hearing from you, *F >> *F >>Gillian *F It was simply done by cloning an EcoRI-NotI fragment (about 800 bp) from *F the pEGFP-N1 vector of Clontech into the pUAST vector (Brand and Perrimon). *F pEGFP-N is the one with the 'enhanced GFP', which contains two aa *F substitutions: *F Phe-64 to Leu and Ser65 to Thr. *F We got a few transformants, all with inserts on the second chromosome only. *F Maura # *U FBrf0112160 *a Zeidler *b M. *t 1999.11.3 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Nov 03 14:58:09 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 Nov 1999 14:58:09 +0000 *F To: zeidler@rascal.med.harvard.edu *F Subject: sba paper *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 3 Nov 1999 15:00:21 +0000 *F Content-Length: 1556 *F Dear Martin, *F In the 1993 abstract (Zeidler and Mlodzik, 1993, Europ. Dros. Res. *F Conf. 13: Poster D15) you mention an 'F121' transcription unit *F adjacent to F125 - from what you wrote on the update form at the Zurich *F meeting, I think that F121 = TfIIA-S - can you confirm that (I'll *F include this information in FlyBase as personal communication from you *F to FlyBase). *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From zeidler@rascal.med.harvard.edu Wed Nov 03 15:21:56 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 Nov 1999 15:21:56 +0000 *F Mime-Version: 1.0 *F Date: Wed, 3 Nov 1999 10:23:57 -0500 *F To: Gillian Millburn (Genetics) *F From: Martin Zeidler *F Subject: Re: sba paper *F Dear Gillian *F So just to confirm: *F the next transcript adjacent to sba is TFIIA-S which was also *F known as F121 (and variations on 121 such as Tf121). *F Hope that helps. *F Martin *F Martin Zeidler PhD *F Dept. of Genetics *F Harvard Medical School *F 200 Longwood Avenue *F Boston, MA 02115 *F USA *F Tel: (617) 432 7548 *F Fax: (617) 432 7688 *F E-mail: zeidler@rascal.med.harvard.edu # *U FBrf0112165 *a Selleck *b S.B. *t 1999.11.2 *T personal communication to FlyBase *u *F From selleck@u.arizona.edu Tue Nov 02 21:50:42 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 Nov 1999 21:50:42 +0000 *F X-Sender: selleck@pop.u.arizona.edu *F Mime-Version: 1.0 *F Date: Tue, 2 Nov 1999 14:56:42 -0700 *F To: flybase-help@morgan.harvard.edu *F From: 'Scott B. Selleck' *F Subject: FlyBase corrections *F gem is a dally allele, failing to complement all dally alleles we *F have tested. *F Sincerely, *F Scott B. Selleck, MD, PhD *F Associate Professor *F Dept. of Molecular & Cellular Biology *F University of Arizona *F Tucson, AZ 85721 *F Tel (520) 621-8663 *F FAX (520) 621-3709 *F email: selleck@u.arizona.edu # *U FBrf0112176 *a Bowman *b A. *t 1999.11.11 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Nov 10 11:48:21 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Nov 1999 11:48:21 +0000 *F To: lgoldstein@ucsd.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 10 Nov 1999 11:50:43 +0000 *F Content-Length: 1430 *F Dear Dr. Goldstein, *F I am curating your paper for FlyBase: *F Bowman et al., 1999, J. Cell Biol. 146(1): 165--180 *F I have a couple of questions about the 'l(2)k10408' line. *F In your paper you discovered that this line is actually a deficiency *F that takes out robl and several other genes. This line is one of the *F Berkeley P-element lines and according to our files has a P{lacW} *F insertion at 54C. Do you know where this P{lacW} insertion is in *F relation to the deficiency that you discovered in the line ? Is the *F P{lacW} insertion at one of the deficiency breakpoints or is the *F deficiency a separate event from the P{lacW} insertion ? Do you know *F which gene the P{lacW} insertion is in - from looking at Figure 1 of *F your paper, if the P{lacW} insertion is at one of the deficiency *F breakpoints it looks to me a though it might be in the transcription *F unit of 'GENE 1' ? *F Any information you provide about this line would be recorded in *F FlyBase as a personal communication from you to FlyBase, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From abowman@ucsd.edu Wed Nov 10 17:51:34 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Nov 1999 17:51:34 +0000 *F X-Sender: abowman@popmail.ucsd.edu *F Mime-Version: 1.0 *F Date: Wed, 10 Nov 1999 09:54:34 -0800 *F To: Gillian Millburn (Genetics) *F From: Aaron Bowman *F Subject: Re: FlyBase query *F Regarding your inquiry to our paper 'Bowman et al., 1999, J. Cell Biol. *F 146(1): 165--180' and the 'l(2)k10408' line. We found that the p-element *F is located at the deficiency break point. The p-element is oriented such *F that the sequence off the 3' end of the p-element (using the oientation *F defined by berkeley) is the sequence to the left of the breakpoint *F indicated in figure 1 of our paper. While the sequence flanking the 5' end *F of the p-element reads past the distal (right) break point (this break *F point is not shown in our paper). I will attempt to diagram this below *F (which is in the same orientation as figure 1): *F \--gene 1 --| (proximal/left break) 3' ..... l(2)k10408............5' *F (distal/right break) |---------- *F The closest gene we found to the right of the p-element (and thus also to *F the riht of the deficiecy) is a gene related to a '5'-nucleotidase *F precursor' and the closest gene at the right/distal break (which is within *F the deficiency) is p62GAP (which is also called QKR54B). *F The deficiency associated with robl k10408 is about 36,000 basepairs. And *F has the aproximate genomic structure as follows. *F gene 1, (breakpoint),-gene 2, gene 3, robl, gene 4, QKR54B, (breakpoint), *F 5' nucleotidase precursor, ...... *F Also I have the following information regarding the identity of these genes. *F gene 1 is a putative novel g-protein coupled receptor and is also encoded *F by EST (GM02553). Also the left breakpoint of l(2)k10408 is about 30 *F basepairs to the right of the putative start codon for this gene. *F gene 2 is a putative Trypsin-like Serine Protease *F gene 3 is a robl-like gene which we believe may be a pseudo-gene. *F gene 4 is a transposase-like gene which is represented by multiple ESTs. *F It is also unusual in that it appears to have a ~5kb insertion within the *F middle of the gene encoding a B104 retrotransposon and sequence which is *F found in multiple sites along the drosophila genome (e.g. 35A). This *F sequence appears to have been distributed throughout the drosophila genome *F perhaps by the B104 retrotranspon which appears to flank this sequence at *F all its locations within the genome. Gene 4 appears to splice over this *F unusual B104 retrotransposon containing sequence. *F QKR54B has been described in the literature. *F I hope this information helps. If you have any other questions let me know. *F Sincerely, *F Aaron Bowman *F > *F >>Dear Dr. Goldstein, *F >> *F >>I am curating your paper for FlyBase: *F >> *F >>Bowman et al., 1999, J. Cell Biol. 146(1): 165--180 *F >> *F >>I have a couple of questions about the 'l(2)k10408' line. *F >> *F >>In your paper you discovered that this line is actually a deficiency *F >>that takes out robl and several other genes. This line is one of the *F >>Berkeley P-element lines and according to our files has a P{lacW} *F >>insertion at 54C. Do you know where this P{lacW} insertion is in *F >>relation to the deficiency that you discovered in the line ? Is the *F >>P{lacW} insertion at one of the deficiency breakpoints or is the *F >>deficiency a separate event from the P{lacW} insertion ? Do you know *F >>which gene the P{lacW} insertion is in - from looking at Figure 1 of *F >>your paper, if the P{lacW} insertion is at one of the deficiency *F >>breakpoints it looks to me a though it might be in the transcription *F >>unit of 'GENE 1' ? *F >> *F >>Any information you provide about this line would be recorded in *F >>FlyBase as a personal communication from you to FlyBase, *F >> *F >>I look forward to hearing from you, *F >> *F >>Gillian *F >> *F >>-------------------------------------------------------------- *F >>Gillian Millburn. *F >> *F >>FlyBase (Cambridge), *F >>Department of Genetics, *F >>University of Cambridge, *F >>Downing Street, email: gm119@gen.cam.ac.uk *F >>Cambridge, CB2 3EH, Ph : 01223-333963 *F >>UK. FAX: 01223-333992 *F >>-------------------------------------------------------------- *F > *F > *F Aaron Bowman *F abowman@ucsd.edu *F UCSD School of Medicine *F 9500 Gilman Dr. mailcode - 0683 *F cmm-w 334 *F La Jolla, CA 92093-0683 *F PHONE \# (858) 534-9704 *F FAX \# (858) 534-9701 *F From gm119@gen.cam.ac.uk Thu Nov 11 11:16:33 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 11 Nov 1999 11:16:33 +0000 *F To: abowman@ucsd.edu *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 11 Nov 1999 11:18:58 +0000 *F Content-Length: 1727 *F Dear Aaron, *F thankyou for your reply, its really great and will help clarify things *F in the database. I will record the information in it as a personal *F communication from you to FlyBase. *F I have a few questions about the 'k10408' deficiency just to clarify *F things further. *F 1. do you have names/symbols for 'gene 1', 'gene 2' etc. that are in *F figure 1 of your paper. At the moment I have given them an 'anonymous' *F designation of the format 'anon-54Ba', 'anon-54Bb' etc. - this is what *F we do with transcripts that are shown on a molecular map but aren't *F really named (and then when they are named properly we change the name *F to the 'proper' name). However if you already have symbols for them *F then I could use those. *F Also do you have a symbol for the '5' nucleotidase precursor' that is *F to the right of the deficiency ? *F For gene 1, since you gave me an EST number, then I can call the gene *F 'BEST:GM02553' for 'Berkeley EST GM02553' as a temporary name until it *F gets a 'proper' symbol. *F 2. Could you tell me which ESTs match gene 4 ? *F 3. *F >QKR54B has been described in the literature. *F I'm not sure if I've managed to find which gene you mean - is it *F 'qrk54B' - 'quaking related 54B' which was described in: *F Fyrberg et al., 1998, Biochem. Genet. 36(1-2): 51--64 *F Gillian *F From abowman@ucsd.edu Thu Nov 11 19:15:45 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 11 Nov 1999 19:15:45 +0000 *F X-Sender: abowman@popmail.ucsd.edu *F Mime-Version: 1.0 *F Date: Thu, 11 Nov 1999 10:00:09 -0800 *F To: Gillian Millburn (Genetics) *F From: Aaron Bowman *F Subject: Re: FlyBase query *F Content-Type: multipart/mixed; boundary='=_AAAK1wAAL/w4MFXu' *F Content-Length: 6029 *F I've pasted each of yor questions below; with an answer below each. *F Aaron *F One note before I start, all the genes shown in the figure are *F contained (atleast in part) within the genomic sequence that has been *F submitted to genbank for the robl genomic interval (Accession \# *F AF141921) *F >1. do you have names/symbols for 'gene 1', 'gene 2' etc. that are in *F >figure 1 of your paper. At the moment I have given them an *F 'anonymous' *F >designation of the format 'anon-54Ba', 'anon-54Bb' etc. - this is *F what *F >we do with transcripts that are shown on a molecular map but aren't *F >really named (and then when they are named properly we change the *F name *F >to the 'proper' name). However if you already have symbols for them *F >then I could use those. *F I don't actually have any names or symbols for the genes. As they just *F show similarity to non-drosophila genes, but don't seem to be exact *F homologs of any specific named gene. Given the similarity of gene3 to *F roadblock, perhaps I should chose a name for this one. *F How about : roadblock similar 54B (robls54B) *F Also it should be noted that we have not identified transcripts for *F Gene2 and Gene3 (though we haven't really tried either); their *F existence as 'genes' is based soley on homology between genomic *F sequence and other genes. *F >Also do you have a symbol for the '5' nucleotidase precursor' that is *F >to the right of the deficiency ? *F I do not, but apparently other 5'-Nucleotidase Precursors (from Rat and *F Human and other species are given the symbol : (5'-NT) or (CD73 *F Antigen) or just (CD73)) *F >For gene 1, since you gave me an EST number, then I can call the gene *F >'BEST:GM02553' for 'Berkeley EST GM02553' as a temporary name until *F it *F >gets a 'proper' symbol. *F ok, please note I have sequenced this entire EST. This sequence has *F not been deposited anywhere. I'll paste the sequence of this gene at *F the end of this message. *F >2. Could you tell me which ESTs match gene 4 ? *F There are a total of 7 ESTs which I had identified. A group of 5 *F overlap with each other and are at the 5' of the gene (left most on my *F map in the paper). These then appear to overlap with another two ESTs *F which are at the 3' end of the gene (and are off the right side of my *F map). The ESTs are *F Group of 5 = LD10890, LD15733, LD09893, LD02955, LD03707 *F the other two are = LD13441 and GM03740 *F The order of the overlap is as follows : 5ESTS, LD13441, GM03740 *F sequencing of the 3' of LD03707 (on of the 5 ESTs) identified sequence *F overlapping with GM03740; which placed all these ESTs as belonging to *F the same gene. *F The EST sequence which seems to contain the most coding sequence (the *F basis of saying the gene encodes a transposase-like protein) is LD13441 *F so if you will be naming the gene based upon the ESTs this is probably *F the one to name the gene after. *F >3. QKR54B has been described in the literature. *F > *F >I'm not sure if I've managed to find which gene you mean - is it *F >'qrk54B' - 'quaking related 54B' which was described in: *F > *F >Fyrberg et al., 1998, Biochem. Genet. 36(1-2): 51--64 *F This is the correct gene. *F \-------------------------------------------------------------------------------------------- *F Sequence of EST GM02553 (1875 basepairs) *F CAGTCGGAAATGCGAATAGTTATTGGATCGTTCACCGCATTTCTTTTGCTGTTATTGCAAAACTCAAATGCCGAAATTCCCGGTTGCGACTTCTTCGACACCGTAGATATTTCAAAAGCGCCAAGATTCTCGAACGGATCGTACCTCTACGAAGGCTTGCTGATCCCCGCCCATTTGACAGCTGAATATGACTACAAGCTCCTGGCCGACGATTCGAAGGAGAAGGTGGCGAGCCACGTACGAGGATGTGCCTGCCACCTCAGGCCATGCATTCGGTTTTGTTGC *F Aaron Bowman *F abowman@ucsd.edu *F UCSD School of Medicine *F 9500 Gilman Dr. mailcode - 0683 *F cmm-w 334 *F La Jolla, CA 92093-0683 *F PHONE \# (858) 534-9704 *F FAX \# (858) 534-9701 *F From gm119@gen.cam.ac.uk Tue Nov 16 12:58:09 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 Nov 1999 12:58:09 +0000 *F To: abowman@ucsd.edu *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 16 Nov 1999 13:00:37 +0000 *F Content-Length: 1580 *F Dear Aaron, *F thanks for your reply about the names of the genes in figure 1 of your *F paper and the extra info about ESTs etc. Here's what I think is the *F best solution for naming each gene: *F 1. Gene 1 - this will be called 'BEST:GM02553' for the EST that matches *F it. This name will be replaced once it is named 'properly' by *F someone. *F 2. Gene 2 - since no EST has been identified for this, it will get the *F 'anonymous' name 'anon-54Ba' until it is named properly. *F 3. Gene 3 - since this is similar to robl, I think the symbol and name *F you suggested 'roadblock similar 54B (robls54B)' is fine. I will *F record as a comment in your paper that this may be a pseudogene. *F 4. Gene 4 - I will name this 'BEST:LD13441' as you suggested since the *F EST LD13441 contains the most coding sequence for it. *F 5. 5' nucleotidase precursor. I am reluctant to name this after the *F rat and human genes. I think at the moment I will give the gene the *F valid symbol '5'-nucleotidase-precursor' , i.e. the symbol will be the *F same as the full name - we do this as a temporary measure when people *F give long names to genes but not a short symbol. Then when the gene *F gets a symbol the symbol can change. *F I hope all the symbols are OK with you. *F I'll curate the information in your messages as a personal *F communication from you to FlyBase. *F that's it, thanks for taking so much time over this, *F Gillian # *U FBrf0112177 *a Orr-Weaver *b T. *t 1999.10.30 *T personal communication to FlyBase *u *F From tweaver@wi.mit.edu Sat Oct 30 03:40:22 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 30 Oct 1999 03:40:22 +0100 *F Date: 29 Oct 1999 22:46:00 -0400 *F From: 'Terry Orr-Weaver' *F Return-Receipt-To: 'Terry Orr-Weaver' *F Subject: naming our gene double parked *F To: 'Eileen Underwood' , *F 'Gillian Millburn' , *F 'Michael Ashburner' *F Cc: 'Allyson Whittaker' *F X-Mailer: Mail*Link SMTP for Quarterdeck Mail; Version 4.1.0 *F Content-Length: 1191 *F Dear Eileen, Gillian, and Michael, *F We identified a lethal complementation group and named it double parked (it *F blocks in two phases of the cell cycle- S and M). We found that a previously *F identified lethal mutation with the isolation number l(2)51Ec is an allele of *F dup. An adjacent female sterile, fs(2)PA77, complements l(2)51Ec, so these *F were assumed to be two different genes. In fact, fs(2)PA77 is an allele of *F dup, because it fails to complement our four lethal alleles which are stronger *F than the l(2)51Ec mutation. In addition, we now have cloned the gene and *F sequenced the PA77 mutation and shown it is causes an amino acid change in the *F open reading frame. *F Regards, *F Terry Orr-Weaver # *U FBrf0112241 *a McGinnis *b W. *t 1999.10.7 *T personal communication to FlyBase *u *F Date: Thu, 7 Oct 1999 18:18:33 -0700 *F To: Leyla Bayraktaroglu *F From: William McGinnis *F Re: apt41 *F The mutation that causes the Arginine to Cysteine substitution in *F the apt41 mutant protein is due to a C > T substitution (on the *F top or coding strand) that changes codon \#55 from CGC to TGC. *F # *U FBrf0112243 *a Greig *b S. *c C. *d O'Kane *t 1999.10.21 *T personal communication to FlyBase *u *F Archived. # *U FBrf0112244 *a Colson *b I. *t 1999.10.19 *T personal communication to FlyBase *u *F >From i.colson@ucl.ac.uk Tue Oct 19 12:34:28 1999 *F Envelope-to: m.ashburner@gen.cam.ac.uk *F Delivery-date: Tue, 19 Oct 1999 12:34:28 +0100 *F From: Isabelle Colson *F To: 'm.ashburner' *F Subject: 68C glue gene microsatellite *F Date: Tue, 19 Oct 1999 12:45:25 +0100 *F X-MSMail-Priority: Normal *F X-Priority: 3 *F X-Mailer: Microsoft Internet Mail 4.70.1155 *F MIME-Version: 1.0 *F Content-Transfer-Encoding: 7bit *F Dear Professor Ashburner, *F Thank you for pointing our error about the primer sequences of the 68c glue *F gene sequence.Our genbank entries have now been corrected. There is indeed *F a microsatellite in that gene, (GT)8 at position 6205 of the genbank *F sequence X01918, but we haven't got primers for it. *F Yours sincerely, *F Isabelle *F Isabelle Colson *F University College London, Department of Biology *F Wolfson House, 4 Stephenson way *F London NW1 2HE *F tel (44) 171 380 7429 *F fax (44) 171 3832048 *F email i.colson@ucl.ac.uk *F \* # *U FBrf0112245 *a Kaufman *b T. *t 1999.10.26 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Thom Kaufman, Indiana University *F To: Bloomington Drosophila Stock Center *F Subject: Progenitor of Antp[+R1] and Antp[+R2] *F Date: 26 October 1999 *F *F Information communicated: *F *F Antp[+R1] and Antp[+R2] are both spontaneous revertants of Antp[73b]. *F # *U FBrf0112246 *a Bloomington Drosophila Stock Center *b ?. *t 1999.10.28 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: P{GawB}V55 and P{GawB}V49 map to chromosome 2 *F Date: 28 October 1999 *F *F Background: No map information was available for P{GawB}V55 and P{GawB}V49 when they were donated to the stock center. We have roughly mapped these insertions at the stock center. *F *F Information communicated: *F P{GawB}V55 and P{GawB}V49 map to chromosome 2. A map location within chromosome 2 has not been determined. # *U FBrf0112247 *a Roote *b J. *t 1999.10.28 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Thu Oct 28 13:26:56 1999 *F To: rd120@mole.bio.cam.ac.uk *F Subject: random data *F Rachel, *F Complementation data for some of the l(1)G lines from Goettingen: *F HC133 v64f N110 *F G0196 l v, f v, f *F G0270 l v, f v, f *F G094 v, f v, f v, f *F G0127 l v, f v, f *F G0056 l v, f v, f *F G0072 l l v, f *F G0076 sl, sf v, f v, f *F G0423 v, f v, f v, f *F G0324 sl, f v, f v, f *F l = lethal *F v = viable *F f = fertile *F sl = semi-lethal *F sf = semi-fertile *F John # *U FBrf0112248 *a Jones *b B. *t 1999.11.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Nov 04 19:41:51 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{w+mC=UAS-gcm.J} insertions *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: P{w+mC=UAS-gcm.J} insertions *F The following homozygous viable P{w+mC=UAS-gcm.J} insertions were *F generated by Brad Jones at the University of California, Berkeley for the *F work described in FBrf0084071. *F P{w+mC=UAS-gcm.J}2 chromosome 2 *F P{w+mC=UAS-gcm.J}3 chromosome 3 *F This information accompanied stocks sent by Brad Jones to the Stock Center *F in October, 1999. # *U FBrf0112249 *a Bloomington Drosophila Stock Center *b ?. *t 1999.11.7 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: loomington Drosophila Stock Center *F Subject: Dp(1;4)r[+]* series are all f[+] *F Date: 26 October 1999 *F *F Information communicated: *F *F As a point of clarification, all three of the duplications Dp(1;4)r[+]s, Dp(1;4)r[+]m and Dp(1;4)r[+]l are duplicated for forked as well as rudimentary. *F # *U FBrf0112250 *a Saide *b J.D. *t 1999.10.29 *T personal communication to FlyBase *u *F From jsaide@bu.edu Fri Oct 29 18:44:30 1999 *F To: Rachel Drysdale (Genetics) *F From: 'Judith D. Saide, PhD' *F Subject: Re: Correction of info at *F http://fruitfly.berkeley.edu/sequence-archive.html *F Rachel- *F Thanks for responding. Z(210), which we call zetalin, and *F Z(400/600)are both Z-band proteins in Drosophila flight muscle. THE LOCI FOR *F THE GENES ENCODING THEM HAS NOT BEEN DETERMINED, AND NEITHER ONE OF THEM IS *F REPRESENTED BY GenBank accession \#U51473. The cDNA that localizes to 52D and *F has been partially sequenced (U51473) codes for a previously unidentified *F 225kD protein that is associated with the A-band of indirect flight muscle. *F It is unrelated to any protein, such as Z(210) or Z(400/600) mentioned in *F FBrf0050188 or FBrf0054488. *F The problem is that the clone we isolated and sequenced we thought *F coded for zetalin, but we were mistaken. The title of the unfortunate *F abstract 1997, Molec. Biol. Cell(FBrf0100232) should have read '225kD *F protein, an A-band associated component in Drosophila flight muscle, has *F repeating immunoglobulin C2-like domains' instead of 'Zetalin, a major *F Z-band component....'. We learned of our mistake after making POLYCLONAL *F antibodies to the protein expressed by our cDNA and found that THEY labelled *F neither the Z-band of indirect flight muscle nor the zetalin doublet on *F immunoblots of fly flight muscle separated be SDS-PAGE. THEY labelled the *F A-band region of myofibrils, and on immunoblots THEY bound to a single *F protein band, 225kD, that migrated extremely closely to the lower molecular *F weight component of zetalin. *F The point is that Z(210),aka zetalin,(FBrf0050188), and FBrf0054488 *F should NOT be linked to (U51473)/52D. The link is between a novel 225kD *F A-band associated protein and (U51473/52D). *F Judith D. Saide, Ph.D. *F Dept. of Physiology, L-713 *F Boston Univ. Sch. of Med. *F 80 E. Concord St. *F Boston, MA 02118 *F ph: (617)638-4388 *F FAX: (617)638-4273 # *U FBrf0112252 *a Sousa-Neves *b R. *t 1999.11.10 *T personal communication to FlyBase *u *F From asneves@visualnet.com.br Wed Nov 10 21:14:46 1999 *F To: *F Subject: T(1;4)sc H and Dp(1;Y;4) *F Dear Colleagues, *F The aberration T(1;4) sc H stated as viable in its report is actually homozygous lethal and fails to complement at least 2 genes of the fourth chromosome namely: *F spa-sv, pho. *F Presumably this is a translocation deficient for terminal genes on the fourth. *F About Dp(1;Y;4)spa pol I'd like to say that the y+ in this duplication variegates. Variegation can varies from a single yellow bristle to large portions of the body. *F Regards *F Rui *F From rd120@gen.cam.ac.uk Thu Nov 11 11:02:48 1999 *F To: flybase-updates@morgan.harvard.edu, asneves@visualnet.com.br *F Subject: Re: T(1;4)sc H and Dp(1;Y;4) *F Dear Rui, *F Thank you for your mail. I would like to incorporate your data into *F the relevant sections of the aberration records in FlyBase but need to *F clarify a couple of points first. *F T(1;4)scH (FBab0006547): *F When you say that this deletes sv and pho do you mean that the *F T(1;4)scH as a whole is deleted for sv and pho, or that one of the *F segregants, namely (Ts(1Rt;4Rt)scH: FBab0023009) deletes sv and pho? *F I think you are meaning the former, but I must check. *F Dp(1;4)193 (FBab0003153): *F I think this is the aberration you mean when you wrote 'Dp(1;Y;4)spa *F pol'. Please could you confirm this for me. *F Your mail will be archived as a personal communication to FlyBase, to *F serve as the source for the information that you have kindly sent. *F Many thanks for your help, *F Rachel. *F From asneves@visualnet.com.br Thu Nov 11 16:25:28 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: T(1;4)sc H and Dp(1;Y;4) *F Dear Rachael, *F With Dp(1;Y;4)spapol y+ I meant (FBab0003251). *F This aberration is homozygous viable (excellent viability and fertility) and *F y+ variegates. *F T(1;4)sc H I meant (FBab0006547) bloomington stock BL-4055. *F This stock is homozygous lethal and does not complement sv-spa and pho. It *F seems that there are some other distal genes not complemented by this *F translocation ( I'll send these data to you later if you want). *F T(1;4)sc H complements: *F ciD ( lethality), pangolin, and eyR. *F Another aberration: *F Tp(4;3)f (FBab0010095) BL895. It complements spa-sv but it does not *F complement the lethal combination ci D/pan3 *F Regards *F Rui Sousa-Neves # *U FBrf0112255 *a Roote *b J. *t 1999.11.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 22 20:48:02 1999 *F To: rd120@gen.cam.ac.uk *F Subject: frtz alleles and deletions *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: frtz alleles and deletions *F In correspondence with the Stock Center (9/99), John Roote of Cambridge *F University provided the following information. Deficiencies for frtz have *F the following breakpoints: *F Df(2L)frtz11 22A1;22B6-9 *F Df(2L)frtz14 22A3;22B3 *F Df(2L)frtz17 21E3-4;22B5-7 *F Df(2L)frtz19 22A2-3;22B7 *F Df(2L)frtz24 22A3-4;22A-B1 *F Df(2L)frtz25 22A3-4;22C1-2 *F Allele entries should be created for the following frtz alleles: *F frtz1 *F frtz3 *F frtz3 is a temperature-sensitive allele. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0112256 *a Jones *b B. *t 1999.11.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 22 19:48:32 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{PZ}gcmrA87 *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: P{PZ}gcmrA87 *F In correspondence with the Stock Center (10/99), Brad Jones, Skirball *F Institute, indicated that the P element construct in gcmrA87 is P{PZ}. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0112257 *a Carpenter *b A.T.C. *t 1999.11.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 22 19:40:11 1999 *F To: flybase-updates@morgan.harvard.edu *F Subject: Auricular gene, Au1 allele and TM3 balancer variant *F From: Kevin Cook, Bloomington Stock Center *F Subject: Auricular gene, Au1 allele and TM3 balancer variant *F The following information about a new dominant mutation called Auricular *F (Au1) was compiled from correspondence with Adelaide Carpenter, Cambridge *F University. *F Au1 was isolated on a TM3, Sb1 ryRK chromosome following X *F irradiation of mwh1 P{ry+=ry11}l(3)ry3 e1 ry506/TM3, Sb1 ryRK *F males. The Au1 mutation causes a dominant Bar-like eye phenotype. Eyes *F have a prominent anterior indentation. The portion of the eye dorsal to *F the indentation is larger than the portion ventral to the indentation *F giving an ear-like or 'auricular' shape to the eye. *F Some years subsequent to the induction of the Au1 mutation, the Sb1 *F mutation on the TM3, Sb1 ryRK, Au1 chromosome reverted, presumably *F spontaneously. Heterozygotes for this chromosome show slightly short, thin *F bristles but no other aspects of a Minute; this phenotype may reflect *F partial reversion of Sb1. The TM3, ryRK, Au1 chromosome represents a *F new balancer variant. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0112258 *a Zeitlinger *b J. *t 1999.9.17 *T personal communication to FlyBase *u *F Archived. # *U FBrf0112260 *a Tsai *b C.C. *t 1999.11.24 *T personal communication to FlyBase *u *F From tsai@salk.edu Thu Nov 25 00:07:06 1999 *F To: flybase-help@morgan.harvard.edu *F Subject: info. on SMRTER *F To whom it may concern, *F I am writing this to correct the information regarding the SMRTER *F gene in the fly base. The full name for SMRTER is not 'SANT domain *F protein'. In fact SMRTER is the full name, which should be *F abbreviated as SMR (symbol). It is related to two vertebrate *F co-repressors, SMRT and N-CoR. All three proteins share a conserved *F domain, named 'the SANT domain'. *F I would appreciate your help on making these changes!! *F Sincerely, *F Chih-Cheng Tsai # *U FBrf0122627 *a Bloomington Drosophila Stock Center *b ?. *t 1999.12.16 *T personal communication to FlyBase *u *F Date: 16 Dec 1999 *F *F Subject: P{w[+mW.hs]=GAL4-da.G32} insertion *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F In correspondence with Elisabeth Knust of Heinrich Heine University *F (November, 1999), she indicated that the insertion of *F P{w[+mW.hs]=GAL4-da.G32} used in Wodarz et al. 1995, Cell 82(1):67-76 *F (FBrf0082789) is located on the third chromosome and is homozygous viable. *F # *U FBrf0122628 *a Bloomington Drosophila Stock Center *b ?. *t 1999.12.21 *T personal communication to FlyBase *u *F Date: 21 Dec 1999 *F *F Subject: P{BS3.0} insertions *F *F From: Kevin Cook, Bloomington Stock Center *F *F Francoise Chanut, UCSF, informed me that two insertions of *F P{ry[+t7.2]=BS3.0} (FBtp0000694) are *F *F P{ry[+t7.2]=BS3.0}H1-1 chromosome 2, homozygous viable *F P{ry[+t7.2]=BS3.0}H1-2 chromosome 3, homozygous lethal *F *F The construct and transformation were described in Blackman et al. 1991 *F Development 111:657--666 (FBrf0053806). # *U FBrf0122629 *a Bloomington Drosophila Stock Center *b ?. *t 1999.12.21 *T personal communication to FlyBase *u *F *F Date: 21 Dec 1999 *F *F Subject: P{Hsf[+t8]}1 insertion *F *F From: Kevin Cook, Bloomington Stock Center *F *F A recessive lethal, third chromosome insertion of P{w[+mC]=Hsf[+t8]} *F (FBtp0007863) was named P{w[+mC]=Hsf[+t8]}1 by Mark Mortin, NIH/NCI, in *F November 1999 correspondence. *F # *U FBrf0122630 *a Bloomington Drosophila Stock Center *b ?. *t 1999.12.22 *T personal communication to FlyBase *u *F Date: 22 Dec 1999 *F *F Subject: P[BE1305][w[+]] *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Brower et al. 1995 Development 121(5):1311--1320 (FBrf0078125) described *F the isolation of mew[023] on a chromosome marked with "P[BE1305][w[+]]" *F (subscripted 'BE1305', superscripted '+'). November 1999 correspondence *F with Marc Brabant, Danny Brower's lab, University of Arizona, indicated *F that the insertion is a miniwhite-marked P element inserted within 200 kb *F of mew. *F # *U FBrf0122631 *a Bloomington Drosophila Stock Center *b ?. *t 1999.12.22 *T personal communication to FlyBase *u *F Date: 22 Dec 1999 *F *F Subject: P{EYE-lacZ.H} and P{GAL4-ey.H} insertions *F *F From: Kevin Cook, Bloomington Stock Center *F *F The following information was communicated by Walter Gehring, Universität *F Basel, December 1999. *F *F One insertion of P{ry[+t7.2]=EYE-lacZ.H} isolated in Halder et al. 1998 *F Development 125(12): 2181-2191 (FBrf0103272) was a recessive lethal *F insertion on the second chromosome called P{EYE-lacZ.H}1013. *F *F Two insertions of P{w[+m*]=GAL4-ey.H} isolated in Hazelett et al. 1998 *F Development 1998 125(18):3741-3751 (FBrf0104754) were: *F *F P{GAL4-ey.H}4-8 Strongest GAL4 expressing line. Second chromosome, *F recessive male sterile insertion. *F P{GAL4-ey.H}3-8 Moderate GAL4 expression. Second chromosome, viable, *F fertile insertion. *F # *U FBrf0122632 *a Wilson *b P. *t 1999.12.22 *T personal communication to FlyBase *u *F From: Patricia Wilson *F Date: December 22, 1999 *F 1. gammaTub37CDPL10 (gammaTub37C11) *F The amino acid replacement is E117K. *F 2. Complete sequence analysis of gammaTub37CDRU34 and partial *F sequence analysis of gammaTub37CDRS42 indicates that these alleles *F have the same mutation. # *U FBrf0122633 *a Rabinow *b L. *t 1999.12.15 *T personal communication to FlyBase *u *F Date: Wed, 15 Dec 1999 *F From: Leonard Rabinow *F 1) Doa clones are lethal in both the germ-line and *F soma; 2) there are 2 different protein isoforms of Doa, one of 55 and the *F other of 105 kD; the 55 is nuclear and the 105 is cytoplasmic; 3) protein *F and RNA expression are essentially ubiquitous throughout development, in *F all tissues, although differences in expression levels exist; 4) *F sex-specific expression of the kinase is essentially identical. # *U FBrf0122634 *a Axton *b M. *t 19?? *T personal communication to FlyBase *u *F Personal communication from: Myles Axton, Oxford University *F To: Bloomington Drosophila Stock Center *F Dated: ? *F *F Information communicated: *F *F Insertion symbol P{SRS3.9}2 *F Localization 12E1 -- 12E2 *F Basis for localization in situ # *U FBrf0122635 *a Berkeley Drosophila Genome Project *b ?. *t 1993.6.1 *T personal communication to FlyBase *u *F Personal communication from: Berkeley Drosophila Genome Project *F To: Bloomington Drosophila Stock Center *F Dated: 6/1/1993 *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and Allele symbols. *F *F Information communicated: *F *F Insertion symbol P{PZ}Dlc90F[04091] *F Allele symbol Dlc90F[04091] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}emc[04322] *F Allele symbol emc[04322] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}kst[01318] *F Allele symbol kst[01318] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{lacW}l(2)s1976[s1976] *F Allele symbol l(2)s1976[s1976] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{lacW}l(2)s4989[s4989] *F Allele symbol l(2)s4989[s4989] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)neo22[02281] *F Allele symbol l(3)neo22[02281] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have dark body color *F *F Insertion symbol P{PZ}quag[05089] *F Allele symbol quag[05089] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{lacW}l(2)s2978[s2978] *F Allele symbol l(2)s2978[s2978] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{lacW}l(2)s4831[s4831] *F Allele symbol l(2)s4831[s4831] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{lacW}l(3)j2B8[j2B8] *F Allele symbol l(3)j2B8[j2B8] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have eye defects *F *F Insertion symbol P{PZ}CycA[03946] *F Allele symbol CycA[03946] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}dally[06464] *F Allele symbol dally[06464] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have wing vein defects *F *F Insertion symbol P{PZ}emc[05592] *F Allele symbol emc[05592] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}fs(3)07084[07084] *F Allele symbol fs(3)07084[07084] *F Allele phenotype semi-lethal | recessive, female sterile | recessive *F *F Insertion symbol P{PZ}klu[10052] *F Allele symbol klu[10052] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(2)05282[05282] *F Allele symbol l(2)05282[05282] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(2)rG232[rG232] *F Allele symbol l(2)rG232[rG232] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes hold wings at a 45 degree angle *F *F Insertion symbol P{PZ}l(3)00096[00096] *F Allele symbol l(3)00096[00096] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)00534[00534] *F Allele symbol l(3)00534[00534] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have a multiple bristle phenotype *F *F Insertion symbol P{PZ}l(3)00714[00714] *F Allele symbol l(3)00714[00714] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)00716[00716] *F Allele symbol l(3)00716[00716] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)01618[01618] *F Allele symbol l(3)01618[01618] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)01949[01949] *F Allele symbol l(3)01949[01949] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)02515[02515] *F Allele symbol l(3)02515[02515] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)03644[03644] *F Allele symbol l(3)03644[03644] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)03685[03685] *F Allele symbol l(3)03685[03685] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)03699[03699] *F Allele symbol l(3)03699[03699] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have wing vein defect *F *F Insertion symbol P{PZ}l(3)03847[03847] *F Allele symbol l(3)03847[03847] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)03988[03988] *F Allele symbol l(3)03988[03988] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04026[04026] *F Allele symbol l(3)04026[04026] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04053[04053] *F Allele symbol l(3)04053[04053] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04069[04069] *F Allele symbol l(3)04069[04069] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04226[04226] *F Allele symbol l(3)04226[04226] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04281[04281] *F Allele symbol l(3)04281[04281] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04684[04684] *F Allele symbol l(3)04684[04684] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)04713[04713] *F Allele symbol l(3)04713[04713] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have notched wings *F *F Insertion symbol P{PZ}l(3)05043[05043] *F Allele symbol l(3)05043[05043] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)05057[05057] *F Allele symbol l(3)05057[05057] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)05146[05146] *F Allele symbol l(3)05146[05146] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)05279[05279] *F Allele symbol l(3)05279[05279] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)05652[05652] *F Allele symbol l(3)05652[05652] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have arch-like wings *F *F Insertion symbol P{PZ}l(3)05884[05884] *F Allele symbol l(3)05884[05884] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)06240[06240] *F Allele symbol l(3)06240[06240] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)06264[06264] *F Allele symbol l(3)06264[06264] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)06664[06664] *F Allele symbol l(3)06664[06664] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)06677[06677] *F Allele symbol l(3)06677[06677] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have etched tergites *F *F Insertion symbol P{PZ}l(3)06713[06713] *F Allele symbol l(3)06713[06713] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)06803[06803] *F Allele symbol l(3)06803[06803] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)06911[06911] *F Allele symbol l(3)06911[06911] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)07217[07217] *F Allele symbol l(3)07217[07217] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)07882[07882] *F Allele symbol l(3)07882[07882] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)09070[09070] *F Allele symbol l(3)09070[09070] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)09291[09291] *F Allele symbol l(3)09291[09291] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)09656[09656] *F Allele symbol l(3)09656[09656] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)10418[10418] *F Allele symbol l(3)10418[10418] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)10585[10585] *F Allele symbol l(3)10585[10585] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)10621[10621] *F Allele symbol l(3)10621[10621] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)10631[10631] *F Allele symbol l(3)10631[10631] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)70Da[02402] *F Allele symbol l(3)70Da[02402] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)neo28[00103] *F Allele symbol l(3)neo28[00103] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)rL061[rL061] *F Allele symbol l(3)rL061[rL061] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}msn[03349] *F Allele symbol msn[03349] *F Allele phenotype semi-lethal | recessive, *F homozygotes late to eclose *F *F Insertion symbol P{PZ}osp[rJ571] *F Allele symbol osp[rJ571] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have bent wings *F *F Insertion symbol P{PZ}Rga[03834] *F Allele symbol Rga[03834] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have incomplete posterior *F *F Insertion symbol P{PZ}Rpd3[04556] *F Allele symbol Rpd3[04556] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}sgl[05007] *F Allele symbol sgl[05007] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}sprd[05284] *F Allele symbol sprd[05284] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have bent wings *F *F Insertion symbol P{PZ}trh[10512] *F Allele symbol trh[10512] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}ttk[02667] *F Allele symbol ttk[02667] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have missing bristles and hairs # *U FBrf0122636 *a Bellen *b H. *c W. *d Gehring *t 1999.8.1 *T personal communication to FlyBase *u *F Personal communication from: Hugo Bellen, Baylor College of Medicine and Walter Gehring, *F Universitat Basel *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has substituted *F valid FlyBase nomenclature for insertion and allele symbols. Insertion and allele phenotypes *F have been, in most cases, inferred from the genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{lArB}A100.1M3 *F Insertion phenotype viable | fertile *F Localization 62A9 -- 62B2 *F Localization basis in situ *F *F Insertion symbol P{lArB}A102.2F3 *F Insertion phenotype viable | fertile *F Localization 100F *F Localization basis in situ *F *F Insertion symbol P{lArB}A103.1M2 *F Insertion phenotype unknown *F Localization 53C -- 53D *F Localization basis in situ *F Other/Comments insertion in CyO; blue balancer *F *F Insertion symbol P{lArB}A106.1F1 *F Insertion phenotype unknown *F Localization 7C *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A119.1M3 *F Insertion phenotype unknown *F Localization 85B *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A130.1F2 *F Insertion phenotype unknown *F Localization 58E *F Localization basis in situ *F Other/Comments insertion in CyO; blue balancer *F *F Insertion symbol P{lArB}A131.1F3 *F Insertion phenotype viable | fertile *F Localization 65C -- 65D *F Localization basis in situ *F *F Insertion symbol P{lArB}A135.2F1 *F Insertion phenotype viable | fertile *F Localization 19A *F Localization basis in situ *F *F Insertion symbol P{lArB}A140.1F3 *F Insertion phenotype unknown *F Localization 85E *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A143.1F3 *F Insertion phenotype viable | fertile *F Localization 86B *F Localization basis in situ *F *F Insertion symbol P{lArB}A154.2M3 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lArB}A168.1F1 *F Insertion phenotype viable | fertile *F Localization 1C *F Localization basis in situ *F *F Insertion symbol P{lArB}A171.1F1 *F Insertion phenotype viable | fertile *F Localization 1A *F Localization basis in situ *F *F Insertion symbol P{lArB}A179.4F3 *F Insertion phenotype unknown *F Localization 71A -- 71B *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A189.2F3 *F Insertion phenotype viable | fertile *F Localization 95B *F Localization basis in situ *F *F Insertion symbol P{lArB}A193.5F3 *F Insertion phenotype viable | fertile *F Localization 34D *F Localization basis in situ *F *F Insertion symbol P{lArB}A217.1M2 *F Insertion phenotype unknown *F Localization 45D -- 45E *F Localization basis in situ *F Other/Comments insertion in CyO; blue balancer *F *F Insertion symbol P{lArB}A229.1F1 *F Insertion phenotype viable | fertile *F Localization 1 -- 20 *F Localization basis segregation *F *F Insertion symbol P{lArB}A256.2F3 *F Insertion phenotype unknown *F Localization 91B *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A265.2M3 *F Insertion phenotype unknown *F Localization 69C *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A289.1F1 *F Insertion phenotype viable | fertile *F Localization 1 -- 20 *F Basis for Localization segregation *F *F Insertion symbol P{lArB}A292.2F3 *F Insertion phenotype viable | fertile *F Localization 88A -- 88B *F Localization basis in situ *F *F Insertion symbol P{lArB}A297.1F1 *F Insertion phenotype viable | fertile *F Localization 2D *F Localization basis in situ *F *F Insertion symbol P{lArB}A308.1M3 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lArB}A317.1F1 *F Insertion phenotype viable | fertile *F Localization 19C *F Localization basis in situ *F *F Insertion symbol P{lArB}A321.3M3 *F Insertion phenotype unknown *F Localization 81F *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A350.1M2 *F Insertion phenotype unknown *F Localization 59E -- 59F *F Localization basis in situ *F Other/Comments insertion in CyO; blue balancer *F *F Insertion symbol P{lArB}A353.2M3 *F Insertion phenotype unknown *F Localization 96F5 -- 96F8 *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lArB}A374.2F1 *F Insertion phenotype viable | fertile *F Localization 12B *F Localization basis in situ *F *F Insertion symbol P{lArB}A418.1F1 *F Insertion phenotype viable | fertile *F Localization 3C1 -- 3C07 *F Localization basis in situ *F *F Insertion symbol P{lArB}A461.1F1 *F Insertion phenotype viable | fertile *F Localization 1A *F Localization basis in situ *F *F Insertion symbol P{lArB}A484.1M3 *F Insertion phenotype viable | fertile *F Localization 94D *F Localization basis in situ *F *F Insertion symbol P{lArB}A487.1M3 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lArB}A507.2M2 *F Insertion phenotype unknown *F Localization 35D -- 35E *F Localization basis in situ *F Other/Comments insertion in CyO; blue balancer *F *F Insertion symbol P{lArB}A522.2F1 *F Insertion phenotype viable | fertile *F Localization 6D -- 6E *F Localization basis in situ *F *F Insertion symbol P{lArB}A55.1M3 *F Insertion phenotype viable | fertile *F Localization 61A *F Localization basis in situ *F *F Insertion symbol P{lArB}A77.1M3 *F Insertion phenotype viable | fertile *F Localization 70A *F Localization basis in situ *F *F Insertion symbol P{lArB}B21.1M3 *F Insertion phenotype viable | fertile *F Localization 70F *F Localization basis in situ *F *F Insertion symbol P{lArB}C40.1S3 *F Insertion phenotype viable | fertile *F Localization 100D *F Localization basis in situ *F *F Insertion symbol P{lArB}lz[A27.1F1] *F Allele phenotype viable | fertile *F Localization 8C -- 8D *F Localization basis in situ # *U FBrf0122637 *a Bloomington Drosophila Stock Center *b ?. *t 1999.12.30 *T personal communication to FlyBase *u *F Personal communication from: Bloomington Drosophila Stock Center *F Date: 12/30/99 *F Background: Corrections to previously reported allele phenotypes, *F based on current behavior in stock at Bloomington. *F *F Insertion symbol P{hsneo}l(3)neo35[1] *F Allele symbol l(3)neo35[1] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{lacW}Dad[j1E4] *F Allele symbol Dad[j1E4] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(2)00297[00297] *F Allele symbol l(2)00297[00297] *F Allele phenotype semi-lethal | recessive *F *F Insertion symbol P{PZ}l(3)05050[05050] *F Allele symbol l(3)05050[05050] *F Allele phenotype semi-lethal | recessive # *U FBrf0122638 *a Brand *b A. *t 1995.7.5 *T personal communication to FlyBase *u *F Personal communication from: Andrea Brand, University of Cambridge *F To: Bloomington Drosophila Stock Center *F Dated: 7/5/1995 (via T. Kaufman) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{GawB}AB1 *F Insertion phenotype viable | fertile *F Localization unknown *F Other/Comments GAL4 in salivary gland, basal expression of P{GawB} # *U FBrf0122639 *a Cooley *b L. *c A. *d Spradling *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Lynn Cooley, Yale School of Medicine, and Allan Spradling, *F HHMI/Carnegie Institute *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has substituted *F valid FlyBase nomenclature for insertion and allele symbols. Insertion and allele phenotypes *F have been, in most cases, inferred from the genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{hsneo}102 *F Insertion phenotype viable | fertile *F Localization 61A -- 61B *F Localization basis in situ *F *F Insertion symbol P{hsneo}103 *F Insertion phenotype viable | fertile *F Localization 63C *F Localization basis in situ *F *F Insertion symbol P{hsneo}104 *F Insertion phenotype viable | fertile *F Localization 66E *F Localization basis in situ *F *F Insertion symbol P{hsneo}105 *F Insertion phenotype viable | fertile *F Localization 66B *F Localization basis in situ *F *F Insertion symbol P{hsneo}106 *F Insertion phenotype viable | fertile *F Localization 66D *F Localization basis in situ *F *F Insertion symbol P{hsneo}107 *F Insertion phenotype viable | fertile *F Localization 66E *F Localization basis in situ *F *F Insertion symbol P{hsneo}108 *F Insertion phenotype viable | fertile *F Localization 67B *F Localization basis in situ *F *F Insertion symbol P{hsneo}109 *F Insertion phenotype viable | fertile *F Localization 67C *F Localization basis in situ *F *F Insertion symbol P{hsneo}112 *F Insertion phenotype viable | fertile *F Localization 68F *F Localization basis in situ *F *F Insertion symbol P{hsneo}113 *F Insertion phenotype viable | fertile *F Localization 68F *F Localization basis in situ *F *F Insertion symbol P{hsneo}114 *F Insertion phenotype viable | fertile *F Localization 70C *F Localization basis in situ *F *F Insertion symbol P{hsneo}115 *F Insertion phenotype viable | fertile *F Localization 71A *F Localization basis in situ *F *F Insertion symbol P{hsneo}116 *F Insertion phenotype viable | fertile *F Localization 71E *F Localization basis in situ *F *F Insertion symbol P{hsneo}117 *F Insertion phenotype viable | fertile *F Localization 75B *F Localization basis in situ *F *F Insertion symbol P{hsneo}119 *F Insertion phenotype viable | fertile *F Localization 75D *F Localization basis in situ *F *F Insertion symbol P{hsneo}120 *F Insertion phenotype viable | fertile *F Localization 75F *F Localization basis in situ *F *F Insertion symbol P{hsneo}122 *F Insertion phenotype viable | fertile *F Localization 77C *F Localization basis in situ *F *F Insertion symbol P{hsneo}123 *F Insertion phenotype viable | fertile *F Localization 78A *F Localization basis in situ *F *F Insertion symbol P{hsneo}124 *F Insertion phenotype viable | fertile *F Localization 79B -- 79C *F Localization basis in situ *F *F Insertion symbol P{hsneo}125 *F Insertion phenotype viable | fertile *F Localization 82D *F Localization basis in situ *F *F Insertion symbol P{hsneo}126 *F Insertion phenotype viable | fertile *F Localization 83B1 -- 83B2 *F Localization basis in situ *F *F Insertion symbol P{hsneo}127 *F Insertion phenotype viable | fertile *F Localization 85A *F Localization basis in situ *F *F Insertion symbol P{hsneo}128 *F Insertion phenotype viable | fertile *F Localization 86A *F Localization basis in situ *F *F Insertion symbol P{hsneo}129 *F Insertion phenotype viable | fertile *F Localization 86C *F Localization basis in situ *F *F Insertion symbol P{hsneo}130 *F Insertion phenotype viable | fertile *F Localization 91B *F Localization basis in situ *F *F Insertion symbol P{hsneo}132 *F Insertion phenotype viable | fertile *F Localization 87E *F Localization basis in situ *F *F Insertion symbol P{hsneo}133 *F Insertion phenotype viable | fertile *F Localization 87F -- 88A *F Localization basis in situ *F *F Insertion symbol P{hsneo}134 *F Insertion phenotype viable | fertile *F Localization 88A *F Localization basis in situ *F *F Insertion symbol P{hsneo}135 *F Insertion phenotype viable | fertile *F Localization 88C *F Localization basis in situ *F *F Insertion symbol P{hsneo}136 *F Insertion phenotype viable | fertile *F Localization 88E *F Localization basis in situ *F *F Insertion symbol P{hsneo}137 *F Insertion phenotype viable | fertile *F Localization 88F *F Localization basis in situ *F *F Insertion symbol P{hsneo}138 *F Insertion phenotype viable | fertile *F Localization 89B *F Localization basis in situ *F *F Insertion symbol P{hsneo}139 *F Insertion phenotype viable | fertile *F Localization 89E *F Localization basis in situ *F *F Insertion symbol P{hsneo}140 *F Insertion phenotype viable | fertile *F Localization 90D *F Localization basis in situ *F *F Insertion symbol P{hsneo}141 *F Insertion phenotype viable | fertile *F Localization 91D *F Localization basis in situ *F *F Insertion symbol P{hsneo}142 *F Insertion phenotype viable | fertile *F Localization 91E *F Localization basis in situ *F *F Insertion symbol P{hsneo}144 *F Insertion phenotype viable | fertile *F Localization 92A -- 92B *F Localization basis in situ *F *F Insertion symbol P{hsneo}146 *F Insertion phenotype viable | fertile *F Localization 95C *F Localization basis in situ *F *F Insertion symbol P{hsneo}147 *F Insertion phenotype viable | fertile *F Localization 95F *F Localization basis in situ *F *F Insertion symbol P{hsneo}148 *F Insertion phenotype viable | fertile *F Localization 96D *F Localization basis in situ *F *F Insertion symbol P{hsneo}149 *F Insertion phenotype viable | fertile *F Localization 96F *F Localization basis in situ *F *F Insertion symbol P{hsneo}150 *F Insertion phenotype viable | fertile *F Localization 97F *F Localization basis in situ *F *F Insertion symbol P{hsneo}151 *F Insertion phenotype viable | fertile *F Localization 99F *F Localization basis in situ *F *F Insertion symbol P{hsneo}152 *F Insertion phenotype viable | fertile *F Localization 100A *F Localization basis in situ *F *F Insertion symbol P{hsneo}l(3)neo35[1] *F Allele symbol l(3)neo35[1] *F Allele phenotype lethal | recessive *F Localization 84D -- 84E *F Localization basis in situ *F Other/Comment revertable (L. Cooley) *F *F Insertion symbol P{hsneo}l(3)neo38[1] *F Allele symbol l(3)neo38[1] *F Allele phenotype semi-lethal | recessive *F Localization 86F1 -- 86F11 *F Localization basis in situ *F Other/Comment Stable on Southern (L. Cooley) *F *F Insertion symbol P{hsneo}l(3)neo47[1] *F Allele symbol l(3)neo47[1] *F Allele phenotype semi-lethal | recessive *F Localization 89C *F Localization basis in situ *F Other/Comment Stable on Southern (L. Cooley) *F *F Insertion symbol P{hsneo}l(3)neo51[1] *F Allele symbol l(3)neo51[1] *F Allele phenotype semi-lethal | recessive *F Localization 92A *F Localization basis in situ *F Other/Comment semi-lethal in reversion test (L. Cooley) *F *F Insertion symbol P{hsneo}lap[1] *F Allele symbol lap[1] *F Synonym l(3)neo34[1] *F Allele phenotype semi-lethal | recessive *F Localization 84D1 -- 84D14 *F Localization basis in situ *F *F Insertion symbol P{hsneo}ms(2)neo4[1] *F Allele symbol ms(2)neo4[1] *F Allele phenotype male semi-sterile | recessive *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{hsneo}ms(3)neo5[1] *F Allele symbol ms(3)neo5[1] *F Allele phenotype male semi-sterile | recessive *F Localization 83D *F Localization basis in situ *F *F Insertion symbol P{ry11}1F *F Insertion phenotype viable | fertile *F Localization 1F *F Localization basis in situ *F *F Insertion symbol P{ry11}RY.1 *F Insertion phenotype viable | fertile *F Localization h1 -- h25 (Y chromosome) *F Localization basis segregation *F *F Insertion symbol P{ry11}RY.4 *F Insertion phenotype viable | fertile *F Localization h1 -- h25 (Y chromosome) *F Localization basis segregation *F *F Insertion symbol P{ry1}R305.1 *F Insertion phenotype viable | fertile *F Localization 42E *F Localization basis in situ *F *F Insertion symbol P{ry3}R401.1 *F Insertion phenotype viable | fertile *F Localization h59--h61 *F Localization basis in situ *F *F Insertion symbol P{S38M}7 *F Insertion phenotype viable | fertile *F Localization 97F *F Localization basis in situ *F *F Insertion symbol P{S38Z.247}3 *F Insertion phenotype viable | fertile *F Localization 93A *F Localization basis in situ *F *F Insertion symbol P{S38Z.74}6 *F Insertion phenotype viable | fertile *F Localization 5D--5E *F Localization basis in situ *F *F Insertion symbol P{S6.9}10 *F Insertion phenotype viable | fertile *F Other/Comment Cytological location of insertion is either 2C or 9C, based on *F in situ. *F *F Insertion symbol P{S6.9}2 *F Insertion phenotype viable | fertile *F Localization 6F *F Localization basis in situ # *U FBrf0122640 *a Engels *b W. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: William Engels, University of Wisconsin *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has substituted *F valid FlyBase nomenclature for insertion and allele symbols. Insertion and allele phenotypes *F have been, in most cases, inferred from the genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{CaSpeR}B26.2-16.1a *F Insertion phenotype unknown *F Localization 28E *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-16.1b *F Insertion phenotype unknown *F Localization 46C -- 46E *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-33.1a *F Insertion phenotype unknown *F Localization 28E *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-33.1b *F Insertion phenotype unknown *F Localization 39B *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-36.1a *F Insertion phenotype unknown *F Localization 26A *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-36.1b *F Insertion phenotype unknown *F Localization 28E *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-4.2a *F Insertion phenotype unknown *F Localization 28E *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}B26.2-4.2b *F Insertion phenotype unknown *F Localization 52C *F Localization basis in situ *F Other/Comment Insertion on CyO *F *F Insertion symbol P{CaSpeR}C100.2a *F Insertion phenotype viable | fertile *F Localization 2B *F Localization basis in situ *F *F Insertion symbol P{CaSpeR}C100.2a *F Insertion phenotype viable | fertile (male) *F Localization 2B *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C100.2b *F Insertion phenotype viable | fertile *F Localization 17C *F Localization basis in situ *F *F Insertion symbol P{CaSpeR}C100.2b *F Insertion phenotype viable | fertile (male) *F Localization 17C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C16.1a *F Insertion phenotype viable | fertile (male) *F Localization 17C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C16.1b *F Insertion phenotype viable | fertile (male) *F Localization 19C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C19.1a *F Insertion phenotype viable | fertile (male) *F Localization 6A *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C19.1b *F Insertion phenotype viable | fertile (male) *F Localization 17C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C34.3 *F Insertion phenotype viable | fertile (male) *F Localization 11D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}C75.2 *F Insertion phenotype viable | fertile (male) *F Localization 18F *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx120.10 *F Insertion phenotype viable | fertile (male) *F Localization 17C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx120A.10 *F Insertion phenotype viable | fertile (male) *F Localization 2A *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx131.6 *F Insertion phenotype viable | fertile (male) *F Localization 10E *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx134.13 *F Insertion phenotype viable | fertile (male) *F Localization 6D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx134.16 *F Insertion phenotype viable | fertile (male) *F Localization 3D -- 3E *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx20.8 *F Insertion phenotype viable | fertile (male) *F Localization 7D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx20A.4 *F Insertion phenotype viable | fertile (male) *F Localization 1B *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx27.1 *F Insertion phenotype viable | fertile (male) *F Localization 18F *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx30.2 *F Insertion phenotype viable | fertile (male) *F Localization 1B *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx30A.1 *F Insertion phenotype viable | fertile (male) *F Localization 5B *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx31A.2a *F Insertion phenotype viable | fertile (male) *F Localization 1C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx31A.2b *F Insertion phenotype viable | fertile (male) *F Localization 16C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx31A.3 *F Insertion phenotype viable | fertile (male) *F Localization 10E *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx32.4 *F Insertion phenotype viable | fertile (male) *F Localization 12E *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx34.11a *F Insertion phenotype viable | fertile (male) *F Localization 3D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx34.11b *F Insertion phenotype viable | fertile (male) *F Localization 6C -- 6E *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx34.6 *F Insertion phenotype viable | fertile (male) *F Localization 6D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}cx35.1 *F Insertion phenotype viable | fertile (male) *F Localization 6E *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}Sh32.1a *F Insertion phenotype viable | fertile (male) *F Localization 14B *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}Sh32.1b *F Insertion phenotype viable | fertile (male) *F Localization 17C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}Sh8.1a *F Insertion phenotype viable | fertile (male) *F Localization 13E9 -- 13F1 *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{CaSpeR}Sh8.1b *F Insertion phenotype viable | fertile (male) *F Localization 17C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{P-Sal}dm[P0] *F Insertion phenotype viable | fertile (male) *F Localization 3D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{P-Sal}I16 *F Insertion phenotype viable | fertile (male) *F Localization 14C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and fertility *F not specified. *F *F Insertion symbol P{P-Sal}I70 *F Insertion phenotype viable | fertile *F Localization 16C *F Localization basis in situ *F # *U FBrf0122641 *a Fischer *b J. *t 1998.11.25 *T personal communication to FlyBase *u *F Personal communication from: Janice Fischer, University of Texas at Austin *F To: Bloomington Drosophila Stock Center *F Dated: 11/25/1998 *F *F Information communicated: *F *F Insertion Symbol P{faf-lacZ.F}JF1 *F Insertion Phenotype viable | fertile *F Localization 1 -- 20 *F Basis for localization segregation *F # *U FBrf0122642 *a Gelbart *b W. *t 1997.1.17 *T personal communication to FlyBase *u *F Personal communication from: William Gelbart, Harvard University *F To: Bloomington Drosophila Stock Center *F Dated: 1/17/1997 *F *F Information communicated: *F *F Insertion Symbol H{PDelta2-3}HoP2.1 *F Insertion Phenotype unknown (chromosome is homozygous lethal) *F Localization 21 -- 60 *F Basis for localization segregation *F *F Insertion Symbol H{PDelta2-3}HoP3 *F Insertion Phenotype unknown (chromosome is homozygous lethal) *F Localization 21 -- 60 *F Basis for localization segregation *F *F Insertion Symbol H{PDelta2-3}HoP8 *F Insertion Phenotype viable | fertile *F Localization 1 -- 20 *F Basis for localization segregation *F # *U FBrf0122643 *a Grell *b E.H. *c V. *d Corces *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: E.H. Grell and Victor Corces *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: Recombination mapping data from E.H. Grell via V. Corces, *F the rest from V. Corces. In the preparation of this personal communication *F Bloomington has substituted valid FlyBase nomenclature for insertion and *F allele symbols. Insertion and allele phenotypes have been, in most cases, *F inferred from the genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{Car20y}25F *F Insertion phenotype viable | fertile *F Localization 25F *F Basis for localization in situ *F *F Insertion symbol P{Car20y}48 *F Insertion phenotype viable | fertile *F Localization 2-63.5 *F Basis for localization recombination *F Other/Comments 8.7 mu to the right of Bl *F *F Insertion symbol P{Car20y}66B *F Insertion phenotype viable | fertile *F Localization 3-25.4 *F Basis for localization recombination *F Other/Comments 1.1 mu to the left of h *F *F Insertion symbol P{Car20y}96E *F Insertion phenotype viable | fertile *F Localization 96E *F Basis for localization in situ *F *F Insertion symbol P{Car20y}SF6 *F Insertion phenotype viable | fertile *F Localization 1-59 *F Basis for localization recombination *F Other/Comments near os *F *F Insertion symbol P{Car20y}SF8 *F Insertion phenotype viable | fertile *F Localization 1-0.0 *F Basis for localization recombination *F Other/Comments near sc # *U FBrf0122644 *a Grossniklaus *b U. *t 1992.4.10 *T personal communication to FlyBase *u *F Personal communication from: Ueli Grossniklaus, University of Basel *F To: Bloomington Drosophila Stock Center *F Dated: 4/10/92 *F *F Information communicated: *F *F Insertion symbol P{lArB}A490.2M3 *F Insertion phenotype unknown *F Localization 68F *F Basis for localization in situ *F Other/Comment chromosome is homozygous lethal *F *F Insertion symbol P{lArB}B72.1M3 *F Insertion phenotype viable | fertile *F Localization 64D *F Basis for localization in situ *F # *U FBrf0122645 *a Hafen *b E. *t 1991.5.1 *T personal communication to FlyBase *u *F Personal communication from: Ernst Hafen, Universitat Zurich *F To: Bloomington Drosophila Stock Center *F Dated: 5/1/1991 *F *F Background: In the preparation of this personal communication Bloomington *F has substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{lArB}17 *F Insertion phenotype viable | fertile *F Localization 92F1-3 *F Localization basis in situ *F *F Insertion symbol P{lArB}36 *F Insertion phenotype viable | fertile *F Localization 92D *F Localization basis in situ *F *F Insertion symbol P{lArB}47 *F Insertion phenotype viable | fertile *F Localization 3F *F Localization basis in situ *F *F Insertion symbol P{lArB}99 *F Insertion phenotype viable | fertile *F Localization 66B1-2 *F Localization basis in situ *F *F Insertion symbol P{lArB}103 *F Insertion phenotype viable | fertile *F Localization 12B *F Localization basis in situ *F *F Insertion symbol P{lArB}112 *F Insertion phenotype viable | fertile *F Localization 10B1-2 *F Localization basis in situ *F *F Insertion symbol P{lArB}154 *F Insertion phenotype viable | fertile *F Localization 10B1-2 *F Localization basis in situ *F *F Insertion symbol P{lArB}166 *F Insertion phenotype viable | fertile *F Localization 89F *F Localization basis in situ *F *F Insertion symbol P{lArB}175 *F Insertion phenotype viable | fertile *F Localization 1E3 *F Localization basis in situ *F *F Insertion symbol P{lArB}195 *F Insertion phenotype viable | fertile *F Localization 9E1-3 *F *F Insertion symbol P{lArB}sca[p58] *F Allele symbol sca[p58] *F Localization 49D *F Localization basis in situ *F Other/Comments lacZ expression pattern like sca, complements sca[1] *F *F Insertion symbol P{sev3}215 *F Insertion phenotype viable | fertile *F Localization 20D *F Localization basis in situ *F *F Insertion symbol P{sev3}218 *F Insertion phenotype viable | fertile *F Localization 24C *F Localization basis in situ *F *F Insertion symbol P{sev2}ch21 *F Insertion phenotype viable | fertile *F Localization 46E *F Localization basis in situ *F *F Insertion symbol P{sev2}ch41 *F Insertion phenotype viable | fertile *F Localization 7D1 -- 7D2 *F Localization basis in situ *F *F Insertion symbol P{sev2}ch50 *F Insertion phenotype viable | fertile *F Localization 93B *F Localization basis in situ *F *F Insertion symbol P{sev4}SC.1 *F Insertion phenotype viable | fertile *F Localization 50B *F Localization basis in situ *F *F Insertion symbol P{sev4}SC.2 *F Insertion phenotype viable | fertile *F Localization 42A -- 42B *F Localization basis in situ *F *F Insertion symbol P{sev4}SC.4 *F Insertion phenotype viable | fertile *F Localization 53D -- 53E *F Localization basis in situ *F *F Insertion symbol P{sev5}S11.1 *F Insertion phenotype viable | fertile *F Localization 18 *F Localization basis in situ *F # *U FBrf0122646 *a Jackson *b R. *t 1996.3.24 *T personal communication to FlyBase *u *F Personal communication from: Rob Jackson, Tufts University School of Medicine *F To: Bloomington Drosophila Stock Center *F Dated: 3/24/96 *F *F Information communicated: *F *F Insertion Symbol P{CaSpeR}cx31A.3 *F Other/Comment The chromosome carrying this insertion also carries a *F long-period circadian rhythm mutation, possibly a per[L] *F mutation. *F # *U FBrf0122647 *a Jan *b Y.N. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Yuh Nung Jan, HHMI/University of California at *F San Francisco *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{lacW}A1-2-54 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}A1-3-1 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A1-3-10 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A3-3-25 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A3-3-47 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A3-3-61 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A4-2-5 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}A4-3-40 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A4-3-53 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A5-2-6 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}A6-2-45 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}A6-3-11 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A6-3-2 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}A6-3-69 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}apt[tdf-C5-2-5] *F Allele symbol apt[tdf-C5-2-5] *F Allele phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B1-2-49 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B1-3-40 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B10-2-13 *F Insertion phenotype viable | fertile *F Localization 50C *F Localization basis in situ *F *F Insertion symbol P{lacW}B10-2-3 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B11-3-11 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B11-3-6 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B12-2-6 *F Insertion phenotype viable | fertile *F Localization 53A? *F Localization basis in situ *F *F Insertion symbol P{lacW}B12-3-5 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B3-2-32 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B4-2-27 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B4-3-31 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B4-3-8 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B6-2-25 *F Insertion phenotype viable | fertile *F Localization 51D *F Localization basis in situ *F *F Insertion symbol P{lacW}B6-3-18 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B6-3-23 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B8-3-38 *F Insertion phenotype viable | fertile *F Localization 67C *F Localization basis in situ *F *F Insertion symbol P{lacW}B9-2-33 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B9-3-25 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}B9-3-40 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}B9-3-52 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C2-2-26 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}C3-2-2 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}C3-2-21 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}C3-2-9 *F Insertion phenotype viable | fertile *F Localization 35C *F Localization basis in situ *F *F Insertion symbol P{lacW}C3-3-30 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C3-3-36 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C4-3-20 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C5-2-7 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}C5-3-22 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C6-2-29 *F Insertion phenotype viable | fertile *F Localization 49D -- 50D *F Localization basis in situ *F *F Insertion symbol P{lacW}C6-2-36 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}C6-3-9 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}C7-3-34 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C8-2-17 *F Insertion phenotype viable | fertile *F Localization 52B -- 52D *F Localization basis in situ *F *F Insertion symbol P{lacW}C8-3-37[C8-3-37] *F Allele symbol C8-3-37[C8-3-37] *F Allele phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}C8-3-5 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}caps[E2-3-27] *F Allele symbol caps[E2-3-27] *F Allele phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}D3-3-11 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}E1-2-32 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E1-2-38 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E10-2-26 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E10-2-40 *F Insertion phenotype viable | fertile *F Localization 53D *F Localization basis in situ *F *F Insertion symbol P{lacW}E10-2-41 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E10-3-1 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}E10-3-40 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}E6-2-17 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E6-3-50 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}E6-3-7 *F Insertion phenotype unknown *F Localization 86E *F Localization basis in situ *F Other/Comment chromosome is lethal *F *F Insertion symbol P{lacW}E6-3-7 *F Insertion phenotype viable | fertile *F Localization 86E *F Localization basis in situ *F *F Insertion symbol P{lacW}E7-2-17 *F Insertion phenotype viable | fertile *F Localization 30B *F Localization basis in situ *F *F Insertion symbol P{lacW}E7-3-33 *F Insertion phenotype unknown *F Localization 61 -- 100 *F Localization basis segregation *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lacW}E7-3-49 *F Insertion phenotype viable | fertile *F Localization 89C -- 89D *F Localization basis in situ *F *F Insertion symbol P{lacW}E7-3-52 *F Insertion phenotype viable | fertile *F Localization 79E *F Localization basis in situ *F *F Insertion symbol P{lacW}E7-3-63 *F Insertion phenotype unknown *F Localization 61 -- 100 *F Localization basis segregation *F Other/Comments chromosome is semi-lethal | recessive *F *F Insertion symbol P{lacW}E8-2-17 *F Insertion phenotype viable | fertile *F Localization 52C -- 52D *F Localization basis in situ *F *F Insertion symbol P{lacW}E8-2-18 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E8-3-57 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}E8-3-63 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}E9-2-2 *F Insertion phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{lacW}E9-3-31 *F Insertion phenotype viable | fertile *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{lacW}esg[B7-2-22] *F Allele symbol esg[B7-2-22] *F Allele phenotype viable | fertile *F Localization 35C3 *F Localization basis in situ *F *F Insertion symbol P{lacW}how[E7-3-4] *F Allele symbol how[E7-3-4] *F Allele phenotype viable | fertile *F Localization 93F13 *F Localization basis in situ *F *F Insertion symbol P{lacW}tsh[A3-2-66] *F Allele symbol tsh[A3-2-66] *F Localization 39E -- 40A *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{lacW}vimar[B6-2-30] *F Allele symbol vimar[B6-2-30] *F Allele phenotype viable | fertile *F Localization 21 -- 60 *F Localization basis segregation *F # *U FBrf0122648 *a Klaembt *b C. *t 1995.10.24 *T personal communication to FlyBase *u *F Personal communication from: Christian Klaembt, Universitat Muenster *F To: Bloomington Drosophila Stock Center *F Dated: 10/24/1995 *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion Symbol P{lacZ-un1}loco[rC56] *F Allele Symbol loco[rC56] *F Allele Phenotype viable | fertile *F Localization 94B-C *F Basis for localization in situ *F *F Insertion Symbol P{lwB}AA142 *F Insertion Phenotype viable | fertile *F Localization 66D *F Basis for localization in situ *F Other/Comments excellent marker for midline glial cells *F # *U FBrf0122649 *a Levis *b R. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Robert Levis, Syracuse University *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{wA}4-30 *F Insertion phenotype viable | fertile *F Localization 30C *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-0923+4-49a *F Insertion phenotype viable | fertile *F Localization 41A *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-0923+4-49b *F Insertion phenotype viable | fertile *F Localization 49C *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-0923+4-52a *F Insertion phenotype viable | fertile *F Localization 41A *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-0923+4-52b *F Insertion phenotype viable | fertile *F Localization 52C *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-12 *F Insertion phenotype viable | fertile (male) *F Localization 12B -- 12C *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and *F fertility not specified. *F *F Insertion symbol P{wA[R]}4-20 *F Insertion phenotype viable | fertile (male) *F Localization 20D *F Localization basis in situ *F Other/Comment Maintained in compound-X stock; female viability and *F fertility not specified. *F *F Insertion symbol P{wA[R]}4-3+4-45a *F Insertion phenotype viable | fertile *F Localization 40A *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-3+4-45b *F Insertion phenotype viable | fertile *F Localization 45D *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-3+4-49a *F Insertion phenotype viable | fertile *F Localization 40A *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-3+4-49b *F Insertion phenotype viable | fertile *F Localization 49D *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-34 *F Insertion Phenotype unknown *F Localization 34E *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{wA[R]}4-46 *F Insertion Phenotype unknown *F Localization 46C *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{wA[R]}4-82 *F Insertion phenotype viable | fertile *F Localization 82F *F Localization basis in situ *F *F Insertion symbol P{wA[R]}4-921 *F Insertion Phenotype unknown *F Localization 92B *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{wA[R]}4-94 *F Insertion Phenotype unknown *F Localization 94E *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{wA[R]}4-95 *F Insertion phenotype viable | fertile *F Localization 95F *F Localization basis in situ *F *F Insertion symbol P{wE}sd[E3] *F Insertion phenotype viable | fertile *F Localization 13E -- 13F *F Localization basis in situ *F *F Insertion symbol P{wG}2 *F Insertion phenotype viable | fertile *F Localization 37A *F Localization basis in situ *F *F Insertion symbol P{wG}4 *F Insertion phenotype viable | fertile *F Localization 13E -- 13F *F Localization basis in situ *F # *U FBrf0122650 *a Lis *b J. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: John Lis, Cornell University *F To: Bloomington Drosophila Stock Center *F Subject: assorted P{} insertions *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has substituted valid FlyBase nomenclature for insertion *F and allele symbols. Insertion and allele phenotypes have been, in most cases, inferred from the genotype of the stock. *F *F Information communicated: *F *F Insertion Symbol P{HBDelta-194}84E *F Insertion Phenotype viable | fertile *F Localization 84E *F Localization basis in situ *F *F Insertion Symbol P{HBDelta-23}9E *F Insertion Phenotype viable | fertile *F Localization 9E *F Localization basis in situ *F *F Insertion Symbol P{HBDelta-23}88A *F Insertion Phenotype viable | fertile *F Localization 88A *F Localization basis in situ *F *F Insertion Symbol P{HBDelta-59}8E *F Insertion Phenotype viable | fertile *F Localization 8E *F Localization basis in situ *F *F Insertion Symbol P{HBDelta-89}18F *F Insertion Phenotype viable | fertile *F Localization 18F4 *F Localization basis in situ *F *F Insertion Symbol P{HBDelta-89}31B *F Insertion Phenotype viable | fertile *F Localization 31B *F Localization basis in situ *F *F Insertion Symbol P{HBDelta-89}52B *F Insertion Phenotype viable | fertile *F Localization 52B *F Localization basis in situ *F *F Insertion Symbol P{hsp26-lacZ}84D *F Insertion Phenotype viable | fertile *F Localization 84D *F Localization basis in situ *F *F Insertion Symbol P{Hsp70B+65}23A *F Insertion Phenotype viable | fertile *F Localization 23A *F Localization basis in situ *F *F Insertion Symbol P{Hsp70B-51}71AB *F Insertion Phenotype viable | fertile *F Localization 71A-B *F Localization basis in situ *F # *U FBrf0122651 *a Mardon *b G. *t 1993.3.1 *T personal communication to FlyBase *u *F Personal communication from: Graeme Mardon, Baylor College of Medicine *F To: Bloomington Drosophila Stock Center *F Dated: 3/1/93 *F *F Background: Corrections to previously reported localization of insertions. *F *F Information communicated: *F *F Insertion Symbol P{S6.9}10 *F Localization 9D *F Basis for localization in situ *F *F Insertion Symbol P{SRS3.9}2 *F Localization 9D *F Basis for localization in situ *F *F --Watch_of_Nightingales_589_000-- *F # *U FBrf0122652 *a Merriam *b J. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: John Merriam, University of California at *F Los Angeles *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/90 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion Symbol P{RP49}B1 *F Insertion Phenotype viable | fertile *F Localization 80A *F Basis for localization in situ *F *F Insertion Symbol P{RP49}F2-80A *F Insertion Phenotype viable | fertile *F Localization 80A *F Basis for localization in situ *F # *U FBrf0122653 *a Pauli *b D. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Daniel Pauli, University of Geneva *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion Symbol P{23.1}T134 *F Insertion Phenotype viable | fertile *F Localization 20B -- 20C *F Localization basis in situ *F *F Insertion Symbol P{23.2}T125 *F Insertion Phenotype viable | fertile *F Localization 61A *F Localization basis in situ *F *F Insertion Symbol P{23.2}T99 *F Insertion Phenotype viable | fertile *F Localization 98E *F Localization basis in situ *F *F Insertion Symbol P{23.3}T12 *F Insertion Phenotype viable | fertile *F Localization 1E -- 1F *F Localization basis in situ *F *F Insertion Symbol P{23.3}T72 *F Insertion Phenotype viable | fertile *F Localization 17B *F Localization basis in situ *F *F Insertion Symbol P{23.4}T13B *F Insertion Phenotype viable | fertile *F Localization 49A *F Localization basis in situ *F *F Insertion Symbol P{23.5}T54 *F Insertion Phenotype viable | fertile *F Localization 53D10 -- 53E11 *F Localization basis in situ *F *F Insertion Symbol P{23.5}T57 *F Insertion Phenotype viable | fertile *F Localization 26A *F Localization basis in situ *F *F Insertion Symbol P{26.1}T59 *F Insertion Phenotype viable | fertile *F Localization 1B-C *F Localization basis in situ *F *F Insertion Symbol P{26.2}T1 *F Insertion Phenotype viable | fertile *F Localization 77E *F Localization basis in situ *F *F Insertion Symbol P{26.2}T21 *F Insertion Phenotype viable | fertile *F Localization 12F -- 13A *F Localization basis in situ *F *F Insertion Symbol P{26.2}T9 *F Insertion Phenotype viable | fertile *F Localization 78E -- 78F *F Localization basis in situ *F *F Insertion Symbol P{26.4}T144 *F Insertion Phenotype viable | fertile *F Localization 25A *F Localization basis in situ *F *F Insertion Symbol P{26.4}T154 *F Insertion Phenotype viable | fertile *F Localization 58A *F Localization basis in situ *F *F Insertion Symbol P{27.1}T109 *F Insertion Phenotype viable | fertile *F Localization 92C -- 92D *F Localization basis in situ *F # *U FBrf0122654 *a Rubin *b G. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Gerald Rubin, HHMI/University of California at *F Berkeley *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{Delta0-1}36 *F Insertion phenotype unknown *F Localization 38A *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{hsp0-1}22 *F Insertion phenotype viable | fertile *F Localization 9D *F Localization basis in situ *F *F Insertion symbol P{hsp0-1}8 *F Insertion phenotype viable | fertile *F Localization 2B *F Localization basis in situ *F *F Insertion symbol P{wA}1-1 *F Insertion Phenotype viable | fertile *F Localization 47A *F Localization basis in situ *F *F Insertion symbol P{wA}3-1 *F Insertion Phenotype viable | fertile *F Localization 59B *F Localization basis in situ *F *F Insertion symbol P{wA}4-4 *F Insertion Phenotype viable | fertile *F Localization 100F *F Localization basis in situ *F *F Insertion symbol P{wA[R]}024 *F Insertion phenotype viable | fertile (male) *F Localization 20C *F Localization basis in situ *F Other/Comments Maintained in compound-X stock; female viability and *F fertility not specified. *F *F Insertion symbol P{wA[R]}4-24 *F Insertion Phenotype viable | fertile *F Localization 24C -- 24D *F Localization basis in situ *F *F Insertion symbol P{wA[R]}B133-0923 *F Insertion phenotype viable | fertile *F Localization 41A *F Localization basis in situ *F *F Insertion symbol P{w[aRsLTR]}V24-20D *F Insertion Phenotype viable | fertile *F Localization 52A *F Localization basis in situ *F *F Insertion symbol P{w[aRsLTR]}V24-35 *F Insertion Phenotype viable | fertile *F Localization 60D *F Localization basis in situ *F *F Insertion symbol P{w[aRsLTR]}V3 *F Insertion Phenotype viable | fertile *F Localization 47D *F Localization basis in situ *F *F Insertion symbol P{wB}1-1 *F Insertion Phenotype viable | fertile *F Localization 89A *F Localization basis in situ *F *F Insertion symbol P{wB}1-2 *F Insertion Phenotype viable | fertile *F Localization 17D -- 17E *F Localization basis in situ *F *F Insertion symbol P{wD}1 *F Insertion Phenotype viable | fertile *F Localization 25C *F Localization basis in situ *F *F Insertion symbol P{wE}1 *F Insertion Phenotype viable | fertile *F Localization 19F *F Localization basis in situ *F *F Insertion symbol P{wE}2 *F Insertion Phenotype viable | fertile *F Localization 12B *F Localization basis in situ *F *F Insertion symbol P{wE}5 *F Insertion Phenotype viable | fertile *F Localization 2A *F Localization basis in situ *F *F Insertion symbol P{wF}2 *F Insertion Phenotype viable | fertile *F Localization 86C *F Localization basis in situ *F *F Insertion symbol P{wF}4-1 *F Insertion Phenotype viable | fertile *F Localization 57B *F Localization basis in situ *F *F Insertion symbol P{wF}4-3 *F Insertion Phenotype viable | fertile *F Localization 97B *F Localization basis in situ *F *F Insertion symbol P{wH}1 *F Insertion Phenotype viable | fertile *F Localization 78C -- 78D *F Localization basis in situ *F *F Insertion symbol P{wH}4 *F Insertion Phenotype viable | fertile *F Localization 6A -- 6B *F Localization basis in situ *F *F Insertion symbol P{white-un2}1.3 *F Insertion phenotype unknown *F Localization 60C7 -- 60C8 *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{white-un2}20D *F Insertion phenotype viable | fertile *F Localization 10A *F Localization basis in situ *F *F Insertion symbol P{white-un2}22 *F Insertion phenotype viable | fertile *F Localization 89B *F Localization basis in situ *F *F Insertion symbol P{white-un2}30 *F Insertion phenotype viable | fertile *F Localization 66C *F Localization basis in situ *F *F Insertion symbol P{white-un2}31 *F Insertion phenotype unknown *F Localization 63D *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{white-un2}9.3 *F Insertion phenotype viable | fertile *F Localization 19E *F Localization basis in situ *F *F Insertion symbol P{Delta0-1}48 *F Insertion phenotype unknown *F Localization 2C? *F Localization basis in situ *F Other/Comments chromosome is lethal *F *F Insertion symbol P{w[aRsLTR]}V21 *F Insertion phenotype unknown *F Localization 81F *F Localization basis in situ *F Other/Comments chromosome is lethal *F # *U FBrf0122655 *a Spradling *b A. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Allan Spradling, HHMI/Carnegie Institute *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{A[[10]]O[[31]]}6 *F Insertion phenotype viable | fertile *F Localization 36C *F Localization basis in situ *F *F Insertion symbol P{A[[17]]O[[15]]}3 *F Insertion phenotype viable | fertile *F Localization 4E-F *F Localization basis in situ *F *F Insertion symbol P{A[[18]]O[[5]]}8 *F Insertion phenotype viable | fertile *F Localization 36B-C *F Localization basis in situ *F *F Insertion symbol P{A[[25]]O[[4]]}10 *F Insertion phenotype viable | fertile *F Localization 12C *F Localization basis in situ *F *F Insertion symbol P{BS2.7A}1 *F Insertion phenotype viable | fertile *F Localization 98A *F Localization basis in situ *F *F Insertion symbol P{BS2.7A}3 *F Insertion phenotype viable | fertile *F Localization 18D *F Localization basis in situ *F *F Insertion symbol P{BS2.7B}10 *F Insertion phenotype viable | fertile *F Localization 15D -- 15E *F Localization basis in situ *F *F Insertion symbol P{BS2.7B}12 *F Insertion phenotype viable | fertile *F Localization 1C -- 1D *F Localization basis in situ *F *F Insertion symbol P{BS2.7B}mfs(2)28Aa[11] *F Allele symbol mfs(2)28Aa[11] *F Allele phenotype semi-sterile | recessive *F Localization 28A *F Localization basis in situ *F *F Insertion symbol P{R7.7}4 *F Insertion phenotype viable | fertile *F Localization 6C *F Localization basis in situ *F *F Insertion symbol P{R7.7}7 *F Insertion phenotype viable | fertile *F Localization 17B *F Localization basis in situ *F *F Insertion symbol P{ry10}R601.1 *F Insertion phenotype viable | fertile *F Localization 96A -- 96B *F Localization basis in situ *F *F Insertion symbol P{ry10}R602.1 *F Insertion phenotype viable | fertile *F Localization 98C *F Localization basis in situ *F *F Insertion symbol P{ry11}1F *F Insertion phenotype viable | fertile *F Localization 1F *F Localization basis in situ *F *F Insertion symbol P{ry11}flp[ry4] *F Allele symbol flp[ry4] *F Allele phenotype semi-lethal | recessive, female sterile | recessive, *F male semi-sterile | recessive *F Localization 31B *F Localization basis in situ *F *F Insertion symbol P{ry11}fs(3)302[1] *F Allele symbol fs(3)302[1] *F Allele phenotype semi-lethal | recessive, female sterile | recessive *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{ry11}l(2)ry41[1] *F Allele symbol l(2)ry41[1] *F Allele phenotype semi-lethal | recessive *F Localization 21 -- 60 *F Localization basis segregation *F *F Insertion symbol P{ry11}l(3)ry119[1] *F Allele symbol l(3)ry119[1] *F Allele phenotype semi-lethal | recessive, visible | recessive, *F homozygotes have tiny or no eyes *F Localization 76D *F Localization basis in situ *F *F Insertion symbol P{ry11}ms(3)303[1] *F Allele symbol ms(3)303[1] *F Allele phenotype semi-lethal | recessive, male sterile | recessive *F Localization 61 -- 100 *F Localization basis segregation *F *F Insertion symbol P{ry11}R701.1 *F Insertion phenotype viable | fertile *F Localization 9E *F Localization basis in situ *F *F Insertion symbol P{ry11}R702.1 *F Insertion phenotype viable | fertile *F Localization 1F *F Localization basis in situ *F *F Insertion symbol P{ry11}R704.1 *F Insertion phenotype viable | fertile *F Localization 43A, +? *F Localization basis in situ *F Other/Comments might be multi-insertion line *F *F Insertion symbol P{ry11}R704.2 *F Insertion phenotype viable | fertile *F Localization 1F *F Localization basis in situ *F *F Insertion symbol P{ry11}R704.3 *F Insertion phenotype viable | fertile *F Localization 18A *F Localization basis in situ *F *F Insertion symbol P{ry11}R705.1 *F Insertion phenotype viable | fertile *F Localization 98C *F Localization basis in situ *F *F Insertion symbol P{ry11}RY.1 *F Insertion phenotype viable | fertile (male) *F Localization Y or 1A1 -- 1B2 *F Localization basis segregation *F Other/Comment Insertion into Dp(1;Y)y[+]. Maintained in compound-X *F stock; female viability and fertility not specified. *F *F Insertion symbol P{ry11}RY.4 *F Insertion phenotype viable | fertile (male) *F Localization Y or 1A1 -- 1B2 *F Localization basis segregation *F Other/Comment Insertion into Dp(1;Y)y[+]. Maintained in compound-X *F stock; female viability and fertility not specified. *F *F Insertion symbol P{ry1}R304.1 *F Insertion phenotype viable | fertile *F Localization 43C *F Localization basis in situ *F *F Insertion symbol P{ry1}R305.1 *F Insertion phenotype viable | fertile *F Localization 42E *F Localization basis in situ *F *F Insertion symbol P{ry1}R306.1 *F Insertion phenotype viable | fertile *F Localization 50B *F Localization basis in situ *F *F Insertion symbol P{ry1}R307.1 *F Insertion phenotype viable | fertile *F Localization 87A *F Localization basis in situ *F *F Insertion symbol P{ry1}R311.1 *F Insertion phenotype viable | fertile *F Localization 86D *F Localization basis in situ *F *F Insertion symbol P{ry1}R403.1 *F Insertion phenotype viable | fertile *F Localization 7D *F Localization basis in situ *F *F Insertion symbol P{ry1}R404.2 *F Insertion phenotype viable | fertile *F Localization 9A -- 9D *F Localization basis in situ *F *F Insertion symbol P{ry1}R501.1 *F Insertion phenotype viable | fertile *F Localization 4D *F Localization basis in situ *F *F Insertion symbol P{ry1}Ste[R301.2] *F Allele symbol Ste[R301.2] *F Insertion phenotype viable | fertile *F Localization 12D *F Localization basis in situ *F *F Insertion symbol P{ry3}R401.1 *F Insertion phenotype viable | fertile *F Localization h59 -- h61 (4th chromosome heterochromatin) *F Localization basis in situ *F *F Insertion symbol P{S38M}7 *F Insertion phenotype viable | fertile *F Localization 97F *F Localization basis in situ *F *F Insertion symbol P{S38Z.247}3 *F Insertion phenotype viable | fertile *F Localization 93A *F Localization basis in situ *F *F Insertion symbol P{S38Z.74}6 *F Insertion phenotype viable | fertile *F Localization 5D -- 5E *F Localization basis in situ *F *F Insertion symbol P{S6.9}10 *F Insertion phenotype viable | fertile *F Localization 2C or 9C *F Localization basis in situ *F *F Insertion symbol P{S6.9}11 *F Insertion phenotype viable | fertile *F Localization 16B *F Localization basis in situ *F *F Insertion symbol P{S6.9}2 *F Insertion phenotype viable | fertile *F Localization 6F *F Localization basis in situ *F *F Insertion symbol P{S6.9}l(2)dC9[9] *F Allele symbol l(2)dC9[9] *F Allele phenotype semi-lethal | recessive *F Localization 52F *F Localization basis in situ *F *F Insertion symbol P{S3.8}mfs(3)1[1] *F Allele symbol mfs(3)1[1] *F Allele phenotype male sterile | recessive, female sterile | recessive *F Localization 96B, 84B -- 84C *F Localization basis in situ *F *F Insertion symbol P{S38M}1 *F Insertion phenotype viable | fertile *F Localization 71C *F Localization basis in situ *F *F Insertion symbol P{S38M}3 *F Insertion phenotype viable | fertile *F Localization 96B *F Localization basis in situ *F *F Insertion symbol P{S38Z.2}6 *F Insertion phenotype viable | fertile *F Localization 63C *F Localization basis in situ *F *F Insertion symbol P{SB2.1B}5 *F Insertion phenotype viable | fertile *F Localization 9B *F Localization basis in situ *F *F Insertion symbol P{SB2.1B}6 *F Insertion phenotype viable | fertile *F Localization 13B9 -- 13C1 *F Localization basis in situ *F *F Insertion symbol P{SRS3.9}2 *F Insertion phenotype viable | fertile *F Localization 12B -- 12C *F Localization basis in situ *F *F Insertion symbol P{SRS3.9}3 *F Insertion phenotype viable | fertile *F Localization 99D *F Localization basis in situ *F *F Insertion symbol P{SRS3.9}4 *F Insertion phenotype viable | fertile *F Localization 61E *F Localization basis in situ *F # *U FBrf0122656 *a Wasserman *b S. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Steven Wasserman, University of California at *F San Diego *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion symbol P{lacW}Y061 *F Insertion phenotype viable | fertile *F Localization 87B -- 87C *F Localization basis in situ *F *F Insertion symbol P{lacW}Y1553 *F Insertion phenotype viable | fertile *F Localization 79F *F Localization basis in situ *F *F Insertion symbol P{lacW}Y237 *F Insertion phenotype viable | fertile *F Localization 95 -- 96 *F Localization basis in situ *F *F Insertion symbol P{lacW}Y257 *F Insertion phenotype viable | fertile *F Localization 79C -- 79D *F Localization basis in situ *F *F Insertion symbol P{lacW}Y443 *F Insertion phenotype viable | fertile *F Localization 65F *F Localization basis in situ *F *F Insertion symbol P{lacW}Y565 *F Insertion phenotype viable | fertile *F Localization 88F *F Localization basis in situ *F *F Insertion symbol P{lacW}Y596a *F Insertion phenotype viable | fertile *F Localization 72 *F Localization basis in situ *F *F Insertion symbol P{lacW}Y596b *F Insertion phenotype viable | fertile *F Localization 82 -- 83 *F Localization basis in situ *F *F Insertion symbol P{lacW}Y603 *F Insertion phenotype viable | fertile *F Localization 99B *F Localization basis in situ *F *F Insertion symbol P{lacW}Y615 *F Insertion phenotype viable | fertile *F Localization 85E *F Localization basis in situ *F *F Insertion symbol P{lacW}Y618a *F Insertion phenotype viable | fertile *F Localization 64 *F Localization basis in situ *F *F Insertion symbol P{lacW}Y618b *F Insertion phenotype viable | fertile *F Localization 96A *F Localization basis in situ *F *F Insertion symbol P{lacW}Y640 *F Insertion phenotype viable | fertile *F Localization 85D *F Localization basis in situ *F *F Insertion symbol P{lacW}Y745 *F Insertion phenotype viable | fertile *F Localization 65C -- 65D *F Localization basis in situ *F # *U FBrf0122657 *a Wohlwill *b A. *t 1990.8.1 *T personal communication to FlyBase *u *F Personal communication from: Arthur Wohlwill, University of Illinois Medical Center *F To: Bloomington Drosophila Stock Center *F Dated: 8/1/1990 (via D. Cribbs, HHMI P-element Stock Center) *F *F Background: In the preparation of this personal communication Bloomington has *F substituted valid FlyBase nomenclature for insertion and allele symbols. *F Insertion and allele phenotypes have been, in most cases, inferred from the *F genotype of the stock. *F *F Information communicated: *F *F Insertion Symbol P{Dpse82}A1 *F Insertion Phenotype unknown *F Localization 85E *F Basis for localization in situ *F Other/Comments chromosome is lethal | recessive and visible | dominant, *F wings held semi-raised *F # *U FBrf0122658 *a Axton *b M. *t 19?? *T personal communication to FlyBase *u *F Personal communication from: Myles Axton, Oxford University *F To: Bloomington Drosophila Stock Center *F Dated: ? *F *F Information communicated: *F *F Insertion symbol P{SRS3.9}2 *F Localization 12E1 -- 12E2 *F Basis for localization in situ # *U FBrf0122659 *a Kiger *b A. *c M. *d Fuller *t 2000.1.10 *T personal communication to FlyBase *u *F Personal communication to FlyBase *F Received from: Amy Kiger, Margaret Fuller *F Department of Developmental Biology *F Stanford University *F Subject: Nup154 DNA sequence annotation *F Date: Jan 10, 2000 *F > >First, I would like to include the positions of all of the P elements *F > >that you mapped. I can't quite discern the exact insertion positions *F > >from the 1999 Kiger et. al. Genetics paper. Could you possibly *F > >provide these? *F TTAGCAGAA (nup-6) GGCGGGAG (nup-4) C (nup-1) GTGGGTAC (nup-5) *F ATTGAGCATCAGTCGACCAAATCTTCAGAT (tlp-1) TAG (tlp-2 and nup-3) TTT *F The insert positions are shown in parentheses. There's a SalI site between *F the nup-5 and tlp-1 insert positions, to orient you. Let me know if you *F need more information. *F > >Second, I would like to include the rescue fragment in the annotation. *F > >>From the methods description it looks like the rescue fragment is a *F > >PstI to XmaI genomic fragment which is said to include 857 bases *F > >upstream and 648 bases downstream of the Nup154 gene. My problem is *F > >that the nearest PstI site that I find in the sequence is about 400 *F > >bases further upstream than what I expected from that statement. *F > >Perhaps there is a polymorphism in the sequence. If you could send me *F > >additional info such as sequence from the 5' end of the rescue *F > >fragment, that would be very helpful. *F You caught an error in our manuscript. The first partial cDNA we isolated *F extended about 400 bases further 5' than the one we confirmed by RACE and *F reported in the paper. We erroneously reported the distance from the PstI *F site to the 5' end of this different cDNA rather than the confirmed start *F site. Instead, as you discovered, the distance is more like 1286 bp *F upstream of the likely nup154 start site. Thanks for catching that. *F > >Do you have any more information about the possible alternatively *F > >spliced intron? It's interesting that it does not use a consensus *F > >splice acceptor site. *F No more information on the suspicious intron. It is possible that this *F intron is an artifact of the cDNA we sequenced (with multiple coverage, so *F we believe that was real). We felt it was fair to mention, however, since *F we reported the \*complete* sequence of one full length cDNA (and it *F happened to contain this difference from the partial cDNAs...). # *U FBrf0122660 *a Blanton *b J. *t 2000.1.20 *T personal communication to FlyBase *u *F *F Personal communication to FlyBase *F Received from: Jason Blanton *F Princeton University *F Subject: dwg sequence annotation *F Date: Jan 20, 2000 *F *F Query from curator: *F *F I have a question from the Gaszner et. al. 1999 Genes and Dev. *F paper. I'd like to check on the molecular changes associated with the *F dwg[90] allele. In Figure 3, the first change is reported as S104P *F while in the text it is reported as S100P. Both of those amino acid *F changes would require two nucleotide substitutions (from AGC to CCN). I *F noticed that the Ser at 99 requires only a single base change to get to *F Pro (TCC to CCC). Can you please tell me which is correct. *F *F The second change is reported as H436Y in the text and H438T in the *F figure. Can you confirm that the change is H438Y. *F *F Reply: *F *F In reference to dwg[90] (Gazner et al 1999 G&D), you are correct. It *F should be S99P and H438Y. *F *F # *U FBrf0122948 *a Apweiler *b R. *t 1999.12.27 *T personal communication to FlyBase *u *F From apweiler@ebi.ac.uk Mon Dec 27 20:09:25 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 27 Dec 1999 20:09:25 +0000 *F Date: Mon, 27 Dec 1999 20:11:32 +0000 *F From: Rolf Apweiler *F Organization: EMBL-EBI *F X-Mailer: Mozilla 4.04 [en] (Win95; I) *F MIME-Version: 1.0 *F To: 'Michael Ashburner (Genetics)' *F CC: apweiler@ebi.ac.uk *F Subject: Re: SWP curiosity *F Content-Transfer-Encoding: 7bit *F *F Hello Michael, *F *F > This is a very curious annotation ! *F > What is the evidence for the change of function ? *F *F Sequence analysis of the whole family. See the Pfam entry and the papers *F mentioned in there as well as the attached Fasta result. *F (http://www2.ebi.ac.uk/servicestmp/25815946324628.html) *F *F Cheers *F *F Rolf *F *F > ID PSD3_DROME STANDARD; PRT; 494 AA. *F > AC P25161; *F > DE PROBABLE 26S PROTEASOME REGULATORY SUBUNIT S3 (DIPHENOL OXIDASE A2 *F > DR PFAM; PF01399; PCI; 1. *F > KW Proteasome. *F > // *F *F FASTA searches a protein or DNA sequence data bank *F version 3.2t09 December 7, 1999 *F Please cite: *F W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 *F *F /net/nfs0/vol1/production/webadmin/tmp/25815946324: 494 aa *F >/net/nfs0/vol1/production/webadmin/tmp/25815946324628.fasta3_t.res.a *F [Unknown form], 494 bases, BE70F752 checksum. *F vs SWISS-PROT All library *F searching /ebi/services/idata/fastadb/swall library *F *F 120063421 residues in 380234 sequences *F statistics extrapolated from 50000 to 380032 sequences *F Expectation_n fit: rho(ln(x))= 5.8847+/-0.000534; mu= 4.9671+/- 0.030; *F mean_var=76.2557+/-14.915, 0's: 147 Z-trim: 24 B-trim: 0 in 0/65 *F *F FASTA (3.28 September 1999) function [optimized, +1/-3 matrix (15:-5)] ktup: 2 *F join: 37, opt: 25, gap-pen: -12/ -2, width: 16 *F Scan time: 117.760 *F The best scores are: initn init1 opt z-sc E(380032) *F *F SWALL:PSD3_DROME P25161 PROBABLE 26S PRO ( 494) 3135 3135 3135 3592.6 1e-192 *F SWALL:O61470 O61470 DIPHENOL OXIDASE-A2. ( 496) 2245 1990 2315 2653.5 2.1e-140 *F *F SWALL:CAB61220 Cab61220 26S PROTEASOME R ( 500) 1976 1942 2234 2560.7 3e-135 *F SWALL:PSD3_MOUSE P14685 26S PROTEASOME R ( 529) 1757 1638 1837 2105.7 6.7e-110 *F *F SWALL:PSD3_HUMAN O43242 26S PROTEASOME R ( 534) 1749 1640 1836 2104.5 7.8e-110 *F *F SWALL:PSD3_TOBAC P93768 26S PROTEASOME R ( 488) 782 459 1328 1523.4 1.8e-77 *F SWALL:PSD3_DAUCA Q06364 26S PROTEASOME R ( 488) 964 391 1171 1343.6 1.9e-67 *F SWALL:PSD3_CAEEL Q04908 PROBABLE 26S PRO ( 504) 1053 797 1122 1287.2 2.6e-64 *F SWALL:PSD3_SCHPO O42897 PROBABLE 26S PRO ( 436) 1057 665 1118 1283.6 4.1e-64 *F SWALL:SUN2_YEAST P40016 26S PROTEASOME R ( 523) 709 505 738 847.3 8.4e-40 *F SWALL:Q9ZSM3 Q9zsm3 PUTATIVE 21D7 PROTEI ( 188) 461 316 566 657.2 3.2e-29 *F SWALL:O95325 O95325 PROTEASOME SUBUNIT P ( 115) 483 483 505 590.6 1.6e-25 *F SWALL:O88543 O88543 COP9 COMPLEX SUBUNIT ( 423) 101 66 195 226.9 3e-05 *F SWALL:O43191 O43191 SIGNALOSOME SUBUNIT ( 403) 101 66 191 222.6 5.2e-05 *F SWALL:AAD41247 Aad41247 COP9 COMPLEX SUB ( 423) 101 66 191 222.3 5.4e-05 *F SWALL:Q40112 Q40112 HYPOTHETICAL 28.4 KD ( 250) 77 69 162 192.6 0.0024 *F SWALL:Q9X4K9 Q9x4k9 PUTATIVE TRANSPOSASE ( 390) 67 67 123 145.0 1.1 *F SWALL:O96149 O96149 PINT DOMAIN PROTEIN ( 459) 55 55 122 142.7 1.5 *F SWALL:AAF00052 Aaf00052 HYPOTHETICAL 31. ( 278) 54 54 111 133.5 4.8 *F SWALL:O25892 O25892 HYPOTHETICAL 26.5 KD ( 224) 86 56 109 132.7 5.3 *F SWALL:Q23320 Q23320 ZC443.1 PROTEIN. 11/ ( 343) 47 47 110 130.9 6.6 *F SWALL:BAA87057 Baa87057 UNCONVENTIONAL M (2167) 79 50 120 130.0 7.5 *F *F >>SWALL:PSD3_DROME P25161 PROBABLE 26S PROTEASOME REGULA (494 aa) *F initn: 3135 init1: 3135 opt: 3135 Z-score: 3592.6 expect() 1e-192 *F Smith-Waterman score: 3135; 100.000% identity in 494 aa overlap (1-494:1-494) *F *F 10 20 30 40 50 60 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQEIREQIRQIEKGVASKESRFILRV *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQEIREQIRQIEKGVASKESRFILRV *F 10 20 30 40 50 60 *F *F 70 80 90 100 110 120 *F /net/n LRNLPNTRRKLNGVVFRNLAQSIYPAGADREAAVALMPAVEKDATELPDVPKKQVATKAP *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: LRNLPNTRRKLNGVVFRNLAQSIYPAGADREAAVALMPAVEKDATELPDVPKKQVATKAP *F 70 80 90 100 110 120 *F *F 130 140 150 160 170 180 *F /net/n IAEVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFSRVAE *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: IAEVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFSRVAE *F 130 140 150 160 170 180 *F *F 190 200 210 220 230 240 *F /net/n LKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSVYPES *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: LKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSVYPES *F 190 200 210 220 230 240 *F *F 250 260 270 280 290 300 *F /net/n ASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLL *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: ASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLL *F 250 260 270 280 290 300 *F *F 310 320 330 340 350 360 *F /net/n GNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRLR *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: GNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRLR *F 310 320 330 340 350 360 *F *F 370 380 390 400 410 420 *F /net/n HNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFMR *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: HNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFMR *F 370 380 390 400 410 420 *F *F 430 440 450 460 470 480 *F /net/n SKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERREREQQDL *F :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: *F SWALL: SKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERREREQQDL *F 430 440 450 460 470 480 *F *F 490 *F /net/n ELAKEMAEDDEDGF *F :::::::::::::: *F SWALL: ELAKEMAEDDEDGF *F 490 *F *F >>SWALL:O61470 O61470 DIPHENOL OXIDASE-A2. 11/99 (496 aa) *F initn: 2245 init1: 1990 opt: 2315 Z-score: 2653.5 expect() 2.1e-140 *F Smith-Waterman score: 2315; 74.793% identity in 484 aa overlap (11-494:17-496) *F *F 10 20 30 40 50 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQEIREQIRQIEKGVASKES *F ::::: .:: ::. .. ::::::.. :::.:.:.::: *F SWALL: MVSQTAAAAAPADPIVDVEME---SAEDAEAAKKDAELLAVQEIRDHARQIDKAVVSKEP *F 10 20 30 40 50 *F *F 60 70 80 90 100 110 *F /net/n RFILRVLRNLPNTRRKLNGVVFRNLAQSIYPAGADREAAVALMPAVEKDATELPDVPKKQ *F ::::::::.::.::::: :: :.:: ..:::: .:.. .: . : : :..:. . *F SWALL: RFILRVLRSLPTTRRKLALVVVRSLAVQLYPAGPERDGIMAYIEDYPAGAQE-PELPRPR *F 60 70 80 90 100 110 *F *F 120 130 140 150 160 170 *F /net/n VATKAPIAEVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFY *F .: :.:. ::::::.:::::.:.: .:: .: .. ::::. ::::.::::.:::::: *F SWALL: AAIKSPVPEVDAYFHLLLLVRLLDKNDLPKATKCSQDLMAKVVGQNRRSLDLIAAKSYFY *F 120 130 140 150 160 170 *F *F 180 190 200 210 220 230 *F /net/n FSRVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKK *F ::::::.:.::.::::::.::::::::::::::::::::::::::::.:::::::::.: *F SWALL: HSRVAELNNDLESIRSFLHSRLRTATLRNDFEGQAVLINCLLRNYLHYSLYDQADKLVNK *F 180 190 200 210 220 230 *F *F 240 250 260 270 280 290 *F /net/n SVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLII *F ::.::.::::: :::::::::::::::::: :::.:::::::.::.::.:::::::::.: *F SWALL: SVFPETASNNECARFLYYLGRIKAAKLEYSVAHKQLVQALRKAPQQAAVGFRQTVQKLVI *F 240 250 260 270 280 290 *F *F 300 310 320 330 340 350 *F /net/n VVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFT *F :::::::.:::: :::::.::.::: :::::::::.:::.:::.:. ..: .:. ::::: *F SWALL: VVELLLGDIPERKVFRQAALRRSLGPYFQLTQAVRMGNLQRFGEVLVNFGEQFRQDHTFT *F 300 310 320 330 340 350 *F *F 360 370 380 390 400 410 *F /net/n LIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDP *F :::::::::::::::::::.::::::::::..: ::: :::::::.:::::::::::::: *F SWALL: LIIRLRHNVIKTAIRSIGLAYSRISPQDIARKLGLDSPEDAEFIVAKAIRDGVIEATLDP *F 360 370 380 390 400 410 *F *F 420 430 440 450 460 470 *F /net/n AQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERRE *F ...::.:::::::::::::::::.::::::.::::::::::::::::::.::::::::: *F SWALL: EKGYMRTKESTDIYSTREPQLAFHQRISFCLDLHNQSVKAMRYPPKSYGKELESAEERRE *F 420 430 440 450 460 470 *F *F 480 490 *F /net/n REQQDLELAKEMAEDDEDGF *F ::::::::::::::.:.::: *F SWALL: REQQDLELAKEMAEEDDDGF *F 480 490 *F *F >>SWALL:CAB61220 Cab61220 26S PROTEASOME REGULATORY SUBU (500 aa) *F initn: 1976 init1: 1942 opt: 2234 Z-score: 2560.7 expect() 3e-135 *F Smith-Waterman score: 2234; 73.620% identity in 489 aa overlap (11-494:19-500) *F *F 10 20 30 40 50 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQEIREQIRQIEKGVASK *F ::::: ::: ::. .. ::::::.. :::.:.:.:: *F SWALL: MVSQTATPTATAASEPIVDVEMESAEDAET---AKKDAELLAVQEIRDHARQIDKAVVSK *F 10 20 30 40 50 *F *F 60 70 80 90 100 *F /net/n ESRFILRVLRNLPNTRRKLNGVVFRNLAQSIYPAGADRE----AAVALMPAVEKDATELP *F : ::::::::.::.::::: :: .:: ..:::: .:. : . .:: .. : *F SWALL: EPRFILRVLRSLPTTRRKLALVVVGSLAVQLYPAGPERDGNQWAYIEDYPAGAQEPEGLH *F 60 70 80 90 100 110 *F *F 110 120 130 140 150 160 *F /net/n DVPKKQVATKAPIA-EVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLI *F : : .. :.:.: ::::::.:::::.:.: .:: .: .. ::::: ::::.:::: *F SWALL: D-PGSD---KSPFAQEVDAYFHLLLLVRLLDKNDLPKATKCSQDLMAKIVGQNRRSLDLI *F 120 130 140 150 160 170 *F *F 170 180 190 200 210 220 *F /net/n GAKSYFYFSRVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQ *F .:: ::: :::.::.:.::.::::::.::::::::::::::::::::::::::::.:::: *F SWALL: AAKCYFYHSRVSELNNDLESIRSFLHSRLRTATLRNDFEGQAVLINCLLRNYLHYSLYDQ *F 180 190 200 210 220 230 *F *F 230 240 250 260 270 280 *F /net/n ADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQ *F :::::.:::.::.::::: :::::::::::::::::: :::.:::::::.::.::.:::: *F SWALL: ADKLVNKSVFPETASNNECARFLYYLGRIKAAKLEYSVAHKQLVQALRKAPQQAAVGFRQ *F 240 250 260 270 280 290 *F *F 290 300 310 320 330 340 *F /net/n TVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKF *F :::::.::::::::.:::: :::::.::.::: :::::::::.:::.:::.:. ..: .: *F SWALL: TVQKLVIVVELLLGDIPERKVFRQAALRRSLGPYFQLTQAVRMGNLQRFGEVLENFGEQF *F 300 310 320 330 340 350 *F *F 350 360 370 380 390 400 *F /net/n QLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGV *F . ::::::::::::::::::::::::.::::::::::..: ::: :::::::.::::::: *F SWALL: RQDHTFTLIIRLRHNVIKTAIRSIGLAYSRISPQDIARKLGLDSPEDAEFIVAKAIRDGV *F 360 370 380 390 400 410 *F *F 410 420 430 440 450 460 *F /net/n IEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLE *F :.::::: ...::.:::::::::::::::::.::::::.::::::::::::::::::.:: *F SWALL: IDATLDPEKGYMRTKESTDIYSTREPQLAFHQRISFCLDLHNQSVKAMRYPPKSYGKELE *F 420 430 440 450 460 470 *F *F 470 480 490 *F /net/n SAEERREREQQDLELAKEMAEDDEDGF *F :::::::::::::::::::::.:.: : *F SWALL: SAEERREREQQDLELAKEMAEEDDDDF *F 480 490 500 *F *F >>SWALL:PSD3_MOUSE P14685 26S PROTEASOME REGULATORY SUBU (529 aa) *F initn: 1757 init1: 1638 opt: 1837 Z-score: 2105.7 expect() 6.7e-110 *F Smith-Waterman score: 1837; 59.175% identity in 485 aa overlap (15-494:47-528) *F *F 10 20 30 40 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQEIREQIRQ *F : : : . ... :......:.:..:: *F SWALL: PPGGEQEPPPPAPQDVEMKEEAAAGSGSTGEGDGKAAATEHSQRELDTVTLEDIKEHVRQ *F 20 30 40 50 60 70 *F *F 50 60 70 80 90 100 *F /net/n IEKGVASKESRFILRVLRNLPNTRRKLNGVVFRNLAQSIYPAG-ADREAAVALMPAVEKD *F .::.:..:: ::.::.:: ::.: :.:: :. . ..... .. : :. :.: .:. *F SWALL: LEKAVSGKEPRFVLRALRMLPSTSRRLNHYVLYKAVHGFFTSNNATRDF---LLPFLEEP *F 80 90 100 110 120 130 *F *F 110 120 130 140 150 *F /net/n ATELPDV---PKKQVATKAPI-AEVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQ *F :. :. :..::. ::.::. ::... :.... :.: .: :: ::: : *F SWALL: MDTEADLQFRPRTGKAASAPLLPEVEAYLQLLMVIFLMNSKRYKEAQKISDDLMQKISTQ *F 140 150 160 170 180 190 *F *F 160 170 180 190 200 210 *F /net/n NRRTLDLIGAKSYFYFSRVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNY *F :.:.:::..:: :.: .:: :. ..:. .::::::::::::::.: .:::.:.: ::::: *F SWALL: NHRALDLVAAKCYYYHARVYEFLDKLDVVRSFLHARLRTATLRHDADGQATLLNLLLRNY *F 200 210 220 230 240 250 *F *F 220 230 240 250 260 270 *F /net/n LHYALYDQADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQ *F :::.:::::.:::.:::.::.:.::::::.::: ::::: .::::.:.. ...::::.:: *F SWALL: LHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSEARRTMTNALRKAPQ *F 260 270 280 290 300 310 *F *F 280 290 300 310 320 330 *F /net/n HAAIGFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDV *F :.:.::.:::.::.::::::::.::.:. ::: .:..:: :: :::::: ::: .:..: *F SWALL: HTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQV *F 320 330 340 350 360 370 *F *F 340 350 360 370 380 390 *F /net/n VSQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIV *F ..:.: ::: : :.:::::::::::::..: :.::::::: :::..:.::: ::::::: *F SWALL: LDQFGEKFQTDGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPEDAEFIV *F 380 390 400 410 420 430 *F *F 400 410 420 430 440 450 *F /net/n SKAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPP *F .::::::::::... .....::: :::::::::::::.::::::..::.::::::.:: *F SWALL: AKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPP *F 440 450 460 470 480 490 *F *F 460 470 480 490 *F /net/n KSYGKDLESAEERREREQQDLELAKEMAEDDEDGF *F :::.::::::::::::::::::.::::::::.:.: *F SWALL: KSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP *F 500 510 520 *F *F >>SWALL:PSD3_HUMAN O43242 26S PROTEASOME REGULATORY SUBU (534 aa) *F initn: 1749 init1: 1640 opt: 1836 Z-score: 2104.5 expect() 7.8e-110 *F Smith-Waterman score: 1836; 58.130% identity in 492 aa overlap (8-494:45-533) *F *F 10 20 30 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQE *F :.. : . .: .. ... :...... *F SWALL: AKPPPGGGEQEPPPPPAPQDVEMKEEAATGGGSTGEADGKTAAAAVEHSQRELDTVTLED *F 20 30 40 50 60 70 *F *F 40 50 60 70 80 90 *F /net/n IREQIRQIEKGVASKESRFILRVLRNLPNTRRKLNGVVFRNLAQSIYPAG-ADREAAVAL *F :.:...:.::.:..:: ::.::.:: ::.: :.:: :. . .:... .. : :. : *F SWALL: IKEHVKQLEKAVSGKEPRFVLRALRMLPSTSRRLNHYVLYKAVQGFFTSNNATRDF---L *F 80 90 100 110 120 130 *F *F 100 110 120 130 140 150 *F /net/n MPAVEKDATELPDV---PKKQVATKAPI-AEVDAYFYLLLLVKLIDASDLKRAGISADAL *F .: .:. :. :. :...:. ::.::. ::... ..... :.: .: : *F SWALL: LPFLEEPMDTEADLQFRPRTGKAASTPLLPEVEAYLQLLVVIFMMNSKRYKEAQKISDDL *F 140 150 160 170 180 190 *F *F 160 170 180 190 200 210 *F /net/n MAKISIQNRRTLDLIGAKSYFYFSRVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLI *F : ::: ::::.:::..:: :.: .:: :. ..:. .::::::::::::::.: .:::.:. *F SWALL: MQKISTQNRRALDLVAAKCYYYHARVYEFLDKLDVVRSFLHARLRTATLRHDADGQATLL *F 200 210 220 230 240 250 *F *F 220 230 240 250 260 270 *F /net/n NCLLRNYLHYALYDQADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQ *F : ::::::::.:::::.:::.:::.::.:.::::::.::: ::::: .::::.:.. ... *F SWALL: NLLLRNYLHYSLYDQAEKLVSKSVFPEQANNNEWARYLYYTGRIKAIQLEYSEARRTMTN *F 260 270 280 290 300 310 *F *F 280 290 300 310 320 330 *F /net/n ALRKSPQHAAIGFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGN *F ::::.:::.:.::.:::.::.::::::::.::.:. ::: .:..:: :: :::::: :: *F SWALL: ALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGN *F 320 330 340 350 360 370 *F *F 340 350 360 370 380 390 *F /net/n LKRFGDVVSQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSA *F : .:..:..:.: ::: : :.:::::::::::::..: :.::::::: :::..:.::: *F SWALL: LAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSP *F 380 390 400 410 420 430 *F *F 400 410 420 430 440 450 *F /net/n EDAEFIVSKAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSV *F :::::::.::::::::::... .....::: :::::::::::::.::::::..::.:: *F SWALL: EDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSV *F 440 450 460 470 480 490 *F *F 460 470 480 490 *F /net/n KAMRYPPKSYGKDLESAEERREREQQDLELAKEMAEDDEDGF *F ::::.:::::.::::::::::::::::::.::::::::.:.: *F SWALL: KAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP *F 500 510 520 530 *F *F >>SWALL:PSD3_TOBAC P93768 26S PROTEASOME REGULATORY SUBU (488 aa) *F initn: 782 init1: 459 opt: 1328 Z-score: 1523.4 expect() 1.8e-77 *F Smith-Waterman score: 1328; 45.825% identity in 491 aa overlap (11-494:4-488) *F *F 10 20 30 40 50 *F /net/n MTNATDIGANDVEM--EVDPTAETLADEKKNQDVAAVQEIREQIRQIEKGVASKESRFIL *F :::: .. : ...:.. . ....: :: :. ..: : : *F SWALL: MTQDVEMKEQAAPPSNSLSSTAPS----IFHHLKEIASLIETGAYAREVRRIS *F 10 20 30 40 *F *F 60 70 80 90 100 110 *F /net/n RVLRNLPNTRRKLNGVVFRNLAQSIYPAGADREAAVA-LMPAVEKDATELPDVPK-KQVA *F :..: :.::.. . . . . :.. .. .. ..: ... :. . . :. *F SWALL: RAVRLTMALRKKLKASSLSAFLNYVLVPGSEVHSRLSSFLPKEDEQDMEVDTATSGAQAP *F 50 60 70 80 90 100 *F *F 120 130 140 150 160 170 *F /net/n TKAPIAEVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFS *F : :. :.. : :::.:. ::: . ..: ..: .:... ::::.:..... :::.: *F SWALL: IKNPLPELEIYCYLLVLIFLIDQKKYNEAKACSSASIARLKTVNRRTVDVLASRLYFYYS *F 110 120 130 140 150 160 *F *F 180 190 200 210 220 230 *F /net/n RVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSV *F :: ..: ::..: : : ::::.: :: .:.: :::::::: :::::.:: .:. *F SWALL: LCYELTGDLAEIRGYLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAP *F 170 180 190 200 210 220 *F *F 240 250 260 270 280 290 *F /net/n YPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVV *F :. ::....:.:.:::.:.. .:::.::.. :.:: ::.:: ::.::: .: :.: *F SWALL: RFEAHSNQQFSRYLFYLGKIRTIQLEYTDAKESLLQAARKAPQ-AALGFRVQCNKWAIIV *F 230 240 250 260 270 280 *F *F 300 310 320 330 340 350 *F /net/n ELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLI *F .::::.::::.:: : :....: ::.::.:::.:.:. : :. ... :. : : .:: *F SWALL: RLLLGEIPERTVFMQKGMEKALRPYFELTNAVRIGDLELFRKVAEKFSSTFSSDGTNNLI *F 290 300 310 320 330 340 *F *F 360 370 380 390 400 410 *F /net/n IRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAE---DAEFIVSKAIRDGVIEATLD *F .:::::::.:..:.:..:::::: :.::.: ::: . ::: :::::::::.:.:::: *F SWALL: VRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPVADAESIVSKAIRDGAIDATLD *F 350 360 370 380 390 400 *F *F 420 430 440 450 460 470 *F /net/n PAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERR *F :...: :::. ::::: :::.::. ::.::::.::..:.:.:.::.:. :. ::::.:: *F SWALL: HANGWMVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRALRFPPNSH-KEKESAEKRR *F 410 420 430 440 450 460 *F *F 480 490 *F /net/n EREQQDLELAKEMAEDDEDGF *F ::.::. ::::..::.:.: : *F SWALL: ERQQQEQELAKHIAEEDDDDF *F 470 480 *F *F >>SWALL:PSD3_DAUCA Q06364 26S PROTEASOME REGULATORY SUBU (488 aa) *F initn: 964 init1: 391 opt: 1171 Z-score: 1343.6 expect() 1.9e-67 *F Smith-Waterman score: 1171; 44.196% identity in 491 aa overlap (11-494:4-488) *F *F 10 20 30 40 50 60 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVAAVQEIREQIRQIEKGVASKESRFILRV *F ::::. : : . .. ...:...: ::.:. ..: : :::. *F SWALL: MTQDVEMKEVP-APAPSNSVTAATPSTLQHLKEIASLIESGAYAREVRRILRA *F 10 20 30 40 50 *F *F 70 80 90 100 110 *F /net/n LRNLPNTRRKLNGVVFRN-LAQSIYPAGADREAAVA-LMPAVEKDATELPDVPKKQVAT- *F .: :.:::. : : :. : :.. .: .: .: .. :. :. . ..: *F SWALL: VRLTIALRKKLNASVVNAFLNFSLVP-GSEVHARLASYLPKEDEHDMEV-DTAMSATTTL *F 60 70 80 90 100 110 *F *F 120 130 140 150 160 170 *F /net/n -KAPIAEVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFS *F : . :.. : :::.:. ::: . ..: ..: .:... ::::....... :::.: *F SWALL: AKHSLPELEIYCYLLVLIFLIDQKKYSEAKACSSASIARVKNLNRRTVEVLASRLYFYYS *F 120 130 140 150 160 170 *F *F 180 190 200 210 220 230 *F /net/n RVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSV *F :: ..: ::. : : : ::::.: :: .:.: :::::::: :::::.:: .:. *F SWALL: LSYELTGDLAEIRGNLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAP *F 180 190 200 210 220 230 *F *F 240 250 260 270 280 290 *F /net/n YPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVV *F :. ::... :.:.:::.:.. .:::.:: :. . : :: .. : . .:.. *F SWALL: RFEAHSNQQFCRYLFYLGKIRTIQLEYTDA-KESCSKLPVSPCCSSW-FPSPMQQVGGDS *F 240 250 260 270 280 *F *F 300 310 320 330 340 350 *F /net/n ELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLI *F ::::.::::.:: : :....: ::.: . .:: . . :..... : :.: .:: *F SWALL: TLLLGEIPERTVFMQKGMEKALRPYFDLQMLYELGIWSSLELVADKFASTFTADRTHNLI *F 290 300 310 320 330 340 *F *F 360 370 380 390 400 410 *F /net/n IRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAE---DAEFIVSKAIRDGVIEATLD *F .:::::::.:..:.:..:::::: :.:..: ::: . ::: :::::::::.:.::.: *F SWALL: VRLRHNVIRTGLRNISISYSRISLVDVARKLRLDSPNPVADAESIVSKAIRDGAIDATID *F 350 360 370 380 390 400 *F *F 420 430 440 450 460 470 *F /net/n PAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERR *F :...: :::. ::::: ::: ::. ::.::::.::..:.:.:.: .:. :: ::::.:: *F SWALL: HANGWMVSKETGDIYSTNEPQAAFNSRIAFCLNMHNEAVRALRFPANSH-KDKESAEKRR *F 410 420 430 440 450 460 *F *F 480 490 *F /net/n EREQQDLELAKEMAEDDEDGF *F ::.::. ::::..::.:.: : *F SWALL: ERQQQEQELAKHIAEEDDDEF *F 470 480 *F *F >>SWALL:PSD3_CAEEL Q04908 PROBABLE 26S PROTEASOME REGULA (504 aa) *F initn: 1053 init1: 797 opt: 1122 Z-score: 1287.2 expect() 2.6e-64 *F Smith-Waterman score: 1194; 42.323% identity in 508 aa overlap (4-494:2-504) *F *F 10 20 30 40 50 *F /net/n MTNATDIGANDVE-MEVDPTAETL--ADEKKNQDVAAVQEIREQIRQIEKGVASKESRFI *F : : . :: :::: . . . .. :. .. ::..:.::. ..:: : ..: *F SWALL: MAPKTGETVVEKMEVDEAKQDVPATEPAKDLNAIAVENIKEQLAALDKG----EEHLI *F 10 20 30 40 50 *F *F 60 70 80 90 100 110 *F /net/n LRVLRNLPNTRRKLNGVVFRNLAQSIYPAGAD-REAAVALMPAVEKDATELPDVPKKQVA *F :::. ::.::...: :. .:..: . :. :. . . . ::. . ..: *F SWALL: TRVLQVLPKTRKQINDNVLYKLVSSHLSSDAQFAEGMLKYLHYTPAADTEVQPMDTSNVK *F 60 70 80 90 100 110 *F *F 120 130 140 150 160 *F /net/n TKAPIA---------EVDAYFYLLLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLI *F ::.: : : :. ::.:..: . .: .. ...: .::::: . *F SWALL: TKSPKKGVKPVFASPESDCYLRLLVLLHLYAQKKNTEALALGENQLTSIYNFDRRTLDGL *F 120 130 140 150 160 170 *F *F 170 180 190 200 210 220 *F /net/n GAKSYFYFSRVAELKNSLEGIRSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQ *F .::. ... . : .. : ..::..::::::::: :.::::: ::: :: :.. *F SWALL: AAKTLYFLCVIYEREGRLFDHQGFLNSRLRTATLRNFSESQAVLICWLLRCYLINRQYQS *F 180 190 200 210 220 230 *F *F 230 240 250 260 270 280 *F /net/n ADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQ *F : .::.: ..:..::::. ::..:: ::::: .:.:..: ...:: ::.::..::::.: *F SWALL: AAHLVSKVAFPDNASNNDLARYMYYQGRIKALQLDYNSAAGYFLQAQRKAPQEGAIGFKQ *F 240 250 260 270 280 290 *F *F 290 300 310 320 330 340 *F /net/n TVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKF *F .::: ..:. :: :.::.: :::: :. :. :..:...:: :.. ::. . :. .: *F SWALL: AVQKWVVVIGLLQGEIPDRSVFRQPIYRKCLAHYLDLSRGVRDGDVARFNHNLEQFKTQF *F 300 310 320 330 340 350 *F *F 350 360 370 380 390 400 *F /net/n QLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGV *F . : :.:::.:::.:::::::..:.:.:::: .::::.:.. . ..:.::.::: ::. *F SWALL: EADDTLTLIVRLRQNVIKTAIKQISLAYSRIYIKDIAKKLYITNETETEYIVAKAIADGA *F 360 370 380 390 400 410 *F *F 410 420 430 440 450 460 *F /net/n IEATLDP----AQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYG *F :.:.. . .:.:.:..::: : ::: : :: .::.::::.:::.:::::. *F SWALL: IDAVITSDVRDGPRYMQSSETADIYRTSEPQAHFDTRIRYCLELHNQAVKALRYPPKKKI *F 420 430 440 450 460 470 *F *F 470 480 490 *F /net/n KDLESAEERREREQQDLELAKEMAEDDEDGF *F .:. :. ::::::.::.:::.:..:.: : *F SWALL: A-VETIEQAREREQQELEFAKELADEDDDDF *F 480 490 500 *F *F >>SWALL:PSD3_SCHPO O42897 PROBABLE 26S PROTEASOME REGULA (436 aa) *F initn: 1057 init1: 665 opt: 1118 Z-score: 1283.6 expect() 4.1e-64 *F Smith-Waterman score: 1118; 45.287% identity in 435 aa overlap (61-491:2-430) *F *F 40 50 60 70 80 *F /net/n DVAAVQEIREQIRQIEKGVASKESRFILRVLRNLPNTRRKLNGVVFRNLAQSIYP-AGAD *F ::. : ..::. :. .: .. : .. *F SWALL: SLRTTSNICHRLNADVLGQLIKKYYSFDNSL *F 10 20 30 *F *F 90 100 110 120 130 140 *F /net/n REAAVAL--MPAVEKDATELPDVPKKQVATKAPIAEVDAYFYLLLLVKLIDASDLKRAGI *F .. . : :: :.. .. . . : : ::: :. ::: . : . .. *F SWALL: KNELLELIDMPQNGDDSST--SITNGNGNTIFP--EVDMYLQLLLSMTLYYNEKYEVGAE *F 40 50 60 70 80 *F *F 150 160 170 180 190 200 *F /net/n SADALMAKISIQNRRTLDLIGAKSYFYFSRVAELKNSLEGIRSFLHARLRTATLRNDFEG *F ..:... .::::: :.:: :::. : : :. : . :::.::.: : *F SWALL: YIKKVIARLQSYDRRTLDQIAAKLYFYYILFFEKCNRSVECRNTLLSVHRTASLRHDSET *F 90 100 110 120 130 140 *F *F 210 220 230 240 250 260 *F /net/n QAVLINCLLRNYLHYALYDQADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAH *F ::.... :::::... ::::::.::.:. . .:::: :. ::::::.: .:.:. :: *F SWALL: QAMVLTLLLRNYIQFNLYDQADRLVSKTSFLTNASNNLAIRYQYYLGRIRAIQLDYTTAH *F 150 160 170 180 190 200 *F *F 270 280 290 300 310 320 *F /net/n KHLVQALRKSPQHA-AIGFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQ *F .:::.:.::.:. . :. : ..: :: :::.::.:.:::: .::: .:...: :....: *F SWALL: EHLVSAIRKAPNTVYAVQFLEAVYKLHIVVQLLMGEIPERRIFRQKSLEKTLVPYLRISQ *F 210 220 230 240 250 260 *F *F 330 340 350 360 370 380 *F /net/n AVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKR *F :::.:.: : :..:.: .:..: .::: ::::.::::..: :.::::::: .:. . *F SWALL: AVRIGDLCAFTDALSKYEAEFRFDGLYTLICRLRHTVIKTGLRMISLSYSRISLRDVCIK *F 270 280 290 300 310 320 *F *F 390 400 410 420 430 440 *F /net/n LMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLN *F : ::: :.::.::.:.::::::.:..: .. :: :.:. :::::..:: ::::::.::: *F SWALL: LGLDSEESAEYIVAKGIRDGVIDASIDHSNAFMASNEAMDIYSTEQPQQAFHERIQFCLA *F 330 340 350 360 370 380 *F *F 450 460 470 480 490 *F /net/n LHNQSVKAMRYPPKSYGKDLESAEERREREQQDLELAKEMAEDDEDGF *F :::.:.:.:::: .. ..::..:: :.: .: :.:. .::: *F SWALL: LHNDSIKSMRYPMDAHKSELEGVEEARRR--MDKEMAEADLDDDEPDLGEF *F 390 400 410 420 430 *F *F >>SWALL:SUN2_YEAST P40016 26S PROTEASOME REGULATORY SUBU (523 aa) *F initn: 709 init1: 505 opt: 738 Z-score: 847.3 expect() 8.4e-40 *F Smith-Waterman score: 837; 32.752% identity in 516 aa overlap (7-492:1-513) *F *F 10 20 30 40 50 *F /net/n MTNATDIGANDVEMEVDPTA--ETLADEKKNQDVAAVQEIREQIRQIEKGVASKESRFIL *F .... : :.:: .. . .::: . :::. . . .: : . . . :.: *F SWALL: MASTAVMMDVDSSGVNDLHHSEKKYAEEDQVQELLKVLNEISKTTLTLDPRYIW *F 10 20 30 40 50 *F *F 60 70 80 90 100 110 *F /net/n RVLRNLPNTRRK--LNGVVFRNLAQSIYPAGAD-REAAVALMPAVEKDAT----ELPD-V *F : :..: . : . ::. .. .. .:: ... .. . .. . .:... :: . *F SWALL: RSLKDLSSLRNQELLNAETLCFTVNVLYPDSSSFKKNLLKFITSNHKSSVPGSAELRNSY *F 60 70 80 90 100 110 *F *F 120 130 140 150 160 *F /net/n PKK--QVAT-KAPI---AEVDAYFYLLLLVKLIDASDLKR-AGISADALMAKI-SIQNRR *F : . .: : : : ::.. ...::. . : :...:.. . .. ... .. : : *F SWALL: PASFYSVNTEKKTIEVTAEINCFMHLLVQLFLWDSKELEQLVEFNRKVVIPNLLCYYNLR *F 120 130 140 150 160 170 *F *F 170 180 190 200 210 *F /net/n TLDLIGAKSYFY-------FSRVAELKNSLEG---IRSFLHARLRTATLRNDFEGQAVLI *F .:.::.:: .:: ..: .: :: . .:: . :. :.:..: : .:.:: *F SWALL: SLNLINAKLWFYIYLSHETLARSSEEINSDNQNIILRSTMMKFLKIASLKHDNETKAMLI *F 180 190 200 210 220 230 *F *F 220 230 240 250 260 270 *F /net/n NCLLRNYLHYALYDQADKLVKKSVYPES-ASNNEWARFLYYLGRIKAAKLEYSDAHKHLV *F : .::..:. . :.:. ...: ::.. .:.. ::...::..:.: .:.:: :..... *F SWALL: NLILRDFLNNGEVDSASDFISKLEYPHTDVSSSLEARYFFYLSKINAIQLDYSTANEYII *F 240 250 260 270 280 290 *F *F 280 290 300 310 320 330 *F /net/n QALRKSPQHA-AIGFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRL *F :.::.:... ..:: : .:: ..::.:.::: :.:.....:: :..::.::.: *F SWALL: AAIRKAPHNSKSLGFLQQSNKLHCCIQLLMGDIPELSFFHQSNMQKSLLPYYHLTKAVKL *F 300 310 320 330 340 350 *F *F 340 350 360 370 380 390 *F /net/n GNLKRFGDVVSQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLD *F ..::.: .....: . : :. : .::: :::::.:: :.:.:..:: .:: .: :: *F SWALL: SDLKKFTSTITKYKQLLLKDDTYQLCVRLRSNVIKTGIRIISLTYKKISLRDICLKLNLD *F 360 370 380 390 400 410 *F *F 400 410 420 430 440 450 *F /net/n SAEDAEFIVSKAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQ *F : . .:..::.::::::::: .. ..:... : .::....:: .: :::.: .::.. *F SWALL: SEQTVEYMVSRAIRDGVIEAKINHEDGFIETTELLNIYDSEDPQQVFDERIKFANQLHDE *F 420 430 440 450 460 470 *F *F 460 470 480 490 *F /net/n SVKAMRYPPKSYGKDLESAEERREREQQDLELAKEMAEDDEDGF *F . .:::: : .. :. .. :..: : .. .: : *F SWALL: YLVSMRYPED---KKTQQNEKSENGENDDDTLDGDLMDDMSDISDLDDLGFL *F 480 490 500 510 520 *F *F >>SWALL:Q9ZSM3 Q9zsm3 PUTATIVE 21D7 PROTEIN (FRAGMENT). (188 aa) *F initn: 461 init1: 316 opt: 566 Z-score: 657.2 expect() 3.2e-29 *F Smith-Waterman score: 566; 52.128% identity in 188 aa overlap (314-494:3-188) *F *F 290 300 310 320 330 340 *F /net/n GFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQL---TQAVRLGNLKRFGDVV *F : ..: :. .: ..:::.:.:. : :. *F SWALL: LTLTKDLTAFTSLLSPSNAVRIGDLSFSGLVA *F 10 20 30 *F *F 350 360 370 380 390 *F /net/n SQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDS---AEDAEF *F ... :. :.: .::.: .. ..:.:..:::::: .:.::.: ::: . ::: *F SWALL: EKFSTTFSSDRTTNLIVRY-DTMWFGGLRNISISYSRISLSDVAKKLRLDSDNPVADAES *F 40 50 60 70 80 90 *F *F 400 410 420 430 440 450 *F /net/n IVSKAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRY *F ::.::::::.:.::.: :...: :::. ::::: ::::::: ::.::::.::..:::.:. *F SWALL: IVAKAIRDGAIDATIDHANGWMISKETGDIYSTNEPQLAFHSRIAFCLNMHNEAVKALRF *F 100 110 120 130 140 150 *F *F 460 470 480 490 *F /net/n PPKSYGKDLESAEERREREQQDLELAKEMAED-DEDGF *F ::.:. :. ::::.::::..:. ::::..::. :.: : *F SWALL: PPNSH-KEKESAEKRRERQHQEQELAKHIAEEEDDDDF *F 160 170 180 *F *F >>SWALL:O95325 O95325 PROTEASOME SUBUNIT P58. 5/99 (115 aa) *F initn: 483 init1: 483 opt: 505 Z-score: 590.6 expect() 1.6e-25 *F Smith-Waterman score: 505; 75.258% identity in 97 aa overlap (398-494:18-114) *F *F 370 380 390 400 410 420 *F /net/n IRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFMRSKESTDI *F ...::::::::::... .....::: :: *F SWALL: MTSPRSCSWIAPKMQSSLLAKAIRDGVIEASINHEKGYVQSKEMIDI *F 10 20 30 40 *F *F 430 440 450 460 470 480 *F /net/n YSTREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERREREQQDLELAKEMA *F :::::::::::.::::::..::.::::::.:::::.::::::::::::::::::.::::: *F SWALL: YSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMA *F 50 60 70 80 90 100 *F *F 490 *F /net/n EDDEDGF *F :::.:.: *F SWALL: EDDDDSFP *F 110 *F *F >>SWALL:O88543 O88543 COP9 COMPLEX SUBUNIT 3. 5/99 (423 aa) *F initn: 101 init1: 66 opt: 195 Z-score: 226.9 expect() 3e-05 *F Smith-Waterman score: 195; 20.859% identity in 326 aa overlap (161-471:94-413) *F *F 140 150 160 170 180 190 *F /net/n LLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFSRVAELKNSLEGIRS *F : . : ... . . ..: :. :.:: *F SWALL: VKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGI *F 70 80 90 100 110 120 *F *F 200 210 220 230 240 *F /net/n FLHA----RLRTATLRN---DFEGQAVLINCLLRNYLHYALYDQADKLVKKSVYPESASN *F . .: .. : : . :. .: .:. . : : :. : ....: . *F SWALL: LKQAIDKMQMNTNQLTSVHADLCQLCLLAKCF-KPALPYLDVDMMDICKENGAY----DA *F 130 140 150 160 170 *F *F 250 260 270 280 290 300 *F /net/n NEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLLGNI *F ... . :: : : .. .. : ::. . .. . .. .: :.: .:::.. *F SWALL: KHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKV *F 180 190 200 210 220 230 *F *F 310 320 330 340 350 *F /net/n PE--RVVFRQAG--LRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRL *F . . . . .: .. .:: .:.:. .: ... ..::... : :... :. . *F SWALL: QQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVSKHSETFTRDNNMGLVKQC *F 240 250 260 270 280 290 *F *F 360 370 380 390 400 410 *F /net/n RHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFM *F .. : :. . .. .: ::.:.:..:.. ..:: : . :.:: : :... .... *F SWALL: LSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMV *F 300 310 320 330 340 350 *F *F 420 430 440 450 460 470 *F /net/n RSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAM----RYPPKSYGKDLESAEERRER *F ... . :.. . ... :..: .. .::: :. :.. : :. *F SWALL: SFHDNPEKYNNPAMLHNIDQEMLKCIEL-DERLKAMDQEITVNPQFVQKSMGSQEDDSGN *F 360 370 380 390 400 410 *F *F 480 490 *F /net/n EQQDLELAKEMAEDDEDGF *F *F SWALL: KPSSYS *F 420 *F *F >>SWALL:O43191 O43191 SIGNALOSOME SUBUNIT 3. 5/99 (403 aa) *F initn: 101 init1: 66 opt: 191 Z-score: 222.6 expect() 5.2e-05 *F Smith-Waterman score: 191; 20.245% identity in 326 aa overlap (161-471:74-393) *F *F 140 150 160 170 180 190 *F /net/n LLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFSRVAELKNSLEGIRS *F : . : ... . . ..: :. :.:: *F SWALL: VKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGI *F 50 60 70 80 90 100 *F *F 200 210 220 230 240 *F /net/n FLHA----RLRT---ATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSVYPESASN *F . .: .. : .... :. .: .:. . : : :. : ....: . *F SWALL: LKQAIDKMQMNTNQLTSIHADLCQLCLLAKCF-KPALPYLDVDMMDICKENGAY----DA *F 110 120 130 140 150 *F *F 250 260 270 280 290 300 *F /net/n NEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLLGNI *F ... . :: : : .. .. : ::. . .. . .. .: :.: .:::.. *F SWALL: KHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKV *F 160 170 180 190 200 210 *F *F 310 320 330 340 350 *F /net/n PE--RVVFRQAG--LRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRL *F . . . . .: .. .:: .:.:. .: ... ..:.... : :... :. . *F SWALL: QQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQC *F 220 230 240 250 260 270 *F *F 360 370 380 390 400 410 *F /net/n RHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFM *F .. : :. . .. .: ::.:.:..:.. ..:: : . :.:: : :... .... *F SWALL: LSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMV *F 280 290 300 310 320 330 *F *F 420 430 440 450 460 470 *F /net/n RSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAM----RYPPKSYGKDLESAEERRER *F ... . :.. . ... :..: .. .::: :. :.. : :. *F SWALL: SFHDNPEKYNNPAMLHNIDQEMLKCIEL-DERLKAMDQEITVNPQFVQKSMGSQEDDSGN *F 340 350 360 370 380 390 *F *F 480 490 *F /net/n EQQDLELAKEMAEDDEDGF *F *F SWALL: KPSSYS *F 400 *F *F >>SWALL:AAD41247 Aad41247 COP9 COMPLEX SUBUNIT 3. 9/99 (423 aa) *F initn: 101 init1: 66 opt: 191 Z-score: 222.3 expect() 5.4e-05 *F Smith-Waterman score: 191; 20.245% identity in 326 aa overlap (161-471:94-413) *F *F 140 150 160 170 180 190 *F /net/n LLLVKLIDASDLKRAGISADALMAKISIQNRRTLDLIGAKSYFYFSRVAELKNSLEGIRS *F : . : ... . . ..: :. :.:: *F SWALL: VKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGI *F 70 80 90 100 110 120 *F *F 200 210 220 230 240 *F /net/n FLHA----RLRT---ATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSVYPESASN *F . .: .. : .... :. .: .:. . : : :. : ....: . *F SWALL: LKQAIDKMQMNTNQLTSIHADLCQLCLLAKCF-KPALPYLDVDMMDICKENGAY----DA *F 130 140 150 160 170 *F *F 250 260 270 280 290 300 *F /net/n NEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLLGNI *F ... . :: : : .. .. : ::. . .. . .. .: :.: .:::.. *F SWALL: KHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKV *F 180 190 200 210 220 230 *F *F 310 320 330 340 350 *F /net/n PE--RVVFRQAG--LRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRL *F . . . . .: .. .:: .:.:. .: ... ..:.... : :... :. . *F SWALL: QQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQC *F 240 250 260 270 280 290 *F *F 360 370 380 390 400 410 *F /net/n RHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFM *F .. : :. . .. .: ::.:.:..:.. ..:: : . :.:: : :... .... *F SWALL: LSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMV *F 300 310 320 330 340 350 *F *F 420 430 440 450 460 470 *F /net/n RSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAM----RYPPKSYGKDLESAEERRER *F ... . :.. . ... :..: .. .::: :. :.. : :. *F SWALL: SFHDNPEKYNNPAMLHNIDQEMLKCIEL-DERLKAMDQEITVNPQFVQKSMGSQEDDSGN *F 360 370 380 390 400 410 *F *F 480 490 *F /net/n EQQDLELAKEMAEDDEDGF *F *F SWALL: KPSSYS *F 420 *F *F >>SWALL:Q40112 Q40112 HYPOTHETICAL 28.4 KD PROTEIN. 5/99 (250 aa) *F initn: 77 init1: 69 opt: 162 Z-score: 192.6 expect() 0.0024 *F Smith-Waterman score: 162; 25.503% identity in 149 aa overlap (287-430:33-181) *F *F 260 270 280 290 300 310 *F /net/n KAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLLG----NIPERVV-FRQ *F .. .: :.: . :: ..:. . : *F SWALL: CIGQKQFRKALELLHNVVTAPMSTLNAIAVEAYKKYILVSLIHLGQFSTSFPKYTSSVAQ *F 10 20 30 40 50 60 *F *F 320 330 340 350 360 370 *F /net/n AGLRQSLGAYFQLTQAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRLRHNVIKTAIRSI *F .:.. :..:... : .... :. ::. :... :.... .. : :. . *F SWALL: RNLKNFSQPYLELSNSYGTGRISELETFVQTNKEKFESDNNLGLVMQVVSSMYKRNIQRL *F 70 80 90 100 110 120 *F *F 380 390 400 410 420 430 *F /net/n GLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFMRSKESTDIYSTR *F .: .: ::::. ..: . ..::. : . :.:: : ::.. ....: :. . :.: *F SWALL: TQTYLTLSLQDIANTVQLRGPKQAEMHVLQMIEDGEIYATINQKDGMVRFLEDPEQYKTC *F 130 140 150 160 170 180 *F *F 440 450 460 470 480 490 *F /net/n EPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERREREQQDLELAKEMAEDDE *F *F SWALL: GMIEHIDSSIKRLMAVSKKLTSMDELMSCDPMYLGKVGRGETEIWILMILTVSLRNSLSE *F 190 200 210 220 230 240 *F *F >>SWALL:Q9X4K9 Q9x4k9 PUTATIVE TRANSPOSASE. 11/99 (390 aa) *F initn: 67 init1: 67 opt: 123 Z-score: 145.0 expect() 1.1 *F Smith-Waterman score: 128; 24.000% identity in 175 aa overlap *F (219-387:185-343) *F *F 190 200 210 220 230 240 *F /net/n RSFLHARLRTATLRNDFEGQAVLINCLLRNYLHYALYDQADKLVKKSVYPESASNNEWAR *F .. .. . .:. . . . :.. :. *F SWALL: SLSGTNYPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSI *F 160 170 180 190 200 210 *F *F 250 260 270 280 290 300 *F /net/n FLYYLGR--IKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLLGNIPER *F :. :: . .:...: ::::: ::.:.::: .:. : : . .. ....::.. *F SWALL: FFEYLKERGLKGTELIISDAHKGLVSAIRKSFTNASW---QRCQ--VHFLRNIFSSIPKK *F 220 230 240 250 260 *F *F 310 320 330 340 350 360 *F /net/n VVFRQAGLRQSLGAYFQLT--QAVRLGNLKRFGDVVSQYGPKFQLDHTFTLIIRLRHNVI *F . .:... : :..: . .: .. :. ..: ::. : .. : . *F SWALL: ---NSKPFREAVKAIFKFTDIELARTAKNALVGEYIDQ--PKY------TKACEILDNGF *F 270 280 290 300 310 *F *F 370 380 390 400 410 420 *F /net/n KTAIR--SIGLSYSRISPQDIAKRLMLDSAEDAEFIVSKAIRDGVIEATLDPAQNFMRSK *F . :.. :: :..:.. .. .:: *F SWALL: EDAFQYTVIGNSHNRLKSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEW *F 320 330 340 350 360 370 *F *F >>SWALL:O96149 O96149 PINT DOMAIN PROTEIN (PROTEASOMAL S (459 aa) *F initn: 55 init1: 55 opt: 122 Z-score: 142.7 expect() 1.5 *F Smith-Waterman score: 122; 21.951% identity in 123 aa overlap *F (249-371:257-373) *F *F 220 230 240 250 260 270 *F /net/n YLHYALYDQADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSP *F : ::.. :::: :..... :. .: *F SWALL: NNMQITSSFLKIINSTDINYSYIPTSFIVLFKNQLGKLYLQKLEYEKAESEFIWAFSNSN *F 230 240 250 260 270 280 *F *F 280 290 300 310 320 330 *F /net/n QHAAIGFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLGAYFQLTQAVRLGNLKRFGD *F . . : ::. . . .:...: : : . ... . .:. :... ... ::. ... *F SWALL: K-SKIEFRKIILESLITIRLNKGLYPPKKLLQ----KYKLSIYIDIIYSIKRGNIFLYNN *F 290 300 310 320 330 340 *F *F 340 350 360 370 380 390 *F /net/n VVSQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFI *F :..... : . . .. :. : ..: . .: *F SWALL: VMNNFSSYF-FHKGLNECIEQIHFIVKRNLIKIVVDWWNKMVQENNQQNKLYKVPIYLFH *F 350 360 370 380 390 400 *F *F 400 410 420 430 440 450 *F /net/n VSKAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYP *F *F SWALL: HIFKWAHITQHHSYLETICIITSLILFRYINAYISYDNNILVLSKNDPFPSLSHNQGPR *F 410 420 430 440 450 *F *F >>SWALL:AAF00052 Aaf00052 HYPOTHETICAL 31.5 KD PROTEIN. (278 aa) *F initn: 54 init1: 54 opt: 111 Z-score: 133.5 expect() 4.8 *F Smith-Waterman score: 111; 24.878% identity in 205 aa overlap (28-220:47-242) *F *F 10 20 30 40 50 *F /net/n MTNATDIGANDVEMEVDPTAETLADEKKNQDVA-AVQEIREQIRQIEKGVASKESRF *F ...:: .....: . . :. :.. : *F SWALL: KIFGDHPIPQYEMNARTTEILYHLSERNRVRDRDVNLVIEDLRPKASEYESE-AKRLEDF *F 20 30 40 50 60 70 *F *F 60 70 80 90 100 110 *F /net/n ILRVLR----NLPNT-RRKLNGVVFRNLAQSIYPAGADREAAVALMPAVEKDATELPDVP *F ... . :: :: : ::..: .: : : : ...:::. ...: . *F SWALL: LMESVNFSPANLSNTGSRFLNALVDSAIALEI----KDTSLA-SFIPAVNDLTSDLFRTK *F 80 90 100 110 120 130 *F *F 120 130 140 150 160 *F /net/n KKQVATKAPIAEVDAYFYLLLLVKLIDASDLKRAGI--SADALMAKISIQNRRTLDLIGA *F .:. : ..... . :... :::.: . ::. :. .:: .:.. : *F SWALL: SKSEEIKLELGKLEKNLTATLVLEKCLREDLKKADVHLSAERAKAEGRLQN---MDFLKA *F 140 150 160 170 180 *F *F 170 180 190 200 210 220 *F /net/n KSY-FYFS-RVAELKNSLEGIR-SFLHARLRTATLR-NDFEGQAVLINCLLRNYLHYALY *F :. : :. :.:: . : .:. :. : : . . . .... :.. .. :..:: *F SWALL: KAAEFRFGIRAAEEQLSSRGMDASLSHRSLVALSDKLSELKQQTIPLKKKLESYLDLMPN *F 190 200 210 220 230 240 *F *F 230 240 250 260 270 280 *F /net/n DQADKLVKKSVYPESASNNEWARFLYYLGRIKAAKLEYSDAHKHLVQALRKSPQHAAIGF *F *F SWALL: PSLAQVKIEEAKRELDAIEAELTKKVDMMEL *F 250 260 270 *F *F >>SWALL:O25892 O25892 HYPOTHETICAL 26.5 KD PROTEIN. 11/9 (224 aa) *F initn: 86 init1: 56 opt: 109 Z-score: 132.7 expect() 5.3 *F Smith-Waterman score: 109; 36.232% identity in 69 aa overlap (371-435:155-221) *F *F 350 360 370 380 390 *F /net/n SQYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAEFI-V *F :::. . .. . ......: :. :..:. . *F SWALL: KKLFNRRISIEEFQNDDFIYHAKQKGVDIKIGLDIATLALKKLVQKIVLISG-DSDFVPA *F 130 140 150 160 170 180 *F *F 400 410 420 430 440 450 *F /net/n SKAIR-DGVIEATLDPAQNFMRS--KESTDIYSTREPQLAFHERISFCLNLHNQSVKAMR *F :: : .:.: :::: : .: :: : .:: ::. *F SWALL: SKLARVEGIIF-TLDPMGNHIREDLKEHIDYLTTRLPQFKQQ *F 190 200 210 220 *F *F 460 470 480 490 *F /net/n YPPKSYGKDLESAEERREREQQDLELAKEMAEDDEDGF *F *F >>SWALL:Q23320 Q23320 ZC443.1 PROTEIN. 11/99 (343 aa) *F initn: 47 init1: 47 opt: 110 Z-score: 130.9 expect() 6.6 *F Smith-Waterman score: 112; 22.156% identity in 167 aa overlap *F (313-477:145-301) *F *F 290 300 310 320 330 340 *F /net/n IGFRQTVQKLIIVVELLLGNIPERVVFRQAGLRQSLG-AYFQLTQAVRLGNLKRFGDVVS *F :: ...: . :. .: ::. :... .: *F SWALL: IPASTKDDGSFRSDVKVEDIWRGFEKVYGLGLTKAIGVSNFNESQIVRIMNIQKVPIHAS *F 120 130 140 150 160 170 *F *F 350 360 370 380 390 400 *F /net/n QYGPKFQLDHTFTLIIRLRHNVIKTAIRSIGLSYSRISPQDIAKRLMLDSAEDAE-FIVS *F : .. : . . .::.. :: ..: : .:.: : ...:....: . . *F SWALL: QLELHLYLPQKAHRELCKKHNILITAYATLG-SPGRMSVVGSNGRPLFESTQNSENEMND *F 180 190 200 210 220 230 *F *F 410 420 430 440 450 460 *F /net/n KAIRDGVIEATLDPAQNFMRSKESTDIYSTREPQLAFHERISFCLNLHNQSVKAMRYPPK *F : .. . . . ::: ..:. : :. . ::.. .:. . ... . *F SWALL: KHVKALAQKYSKTPAQILLRATVEMGIIVI--PKTTNPERMKENINIFDFNISNAEV--- *F 240 250 260 270 280 *F *F 470 480 490 *F /net/n SYGKDLESAEERREREQQDLELAKEMAEDDEDGF *F .: :.:: ..:. *F SWALL: ----NLLEAHERTKQERLFWWPKYFFLSNKNDTEHYFSALLIILKIHSRHRDNKCPKEI *F 290 300 310 320 330 340 *F *F >>SWALL:BAA87057 Baa87057 UNCONVENTIONAL MYOSIN HEAVY CH (2167 aa) *F initn: 79 init1: 50 opt: 120 Z-score: 130.0 expect() 7.5 *F Smith-Waterman score: 120; 27.570% identity in 214 aa overlap *F (286-491:171-368) *F *F 260 270 280 290 300 310 *F /net/n IKAAKLEYSDAHKHLVQALRKSPQHAAIGFRQTVQKLIIVVELLLGNIPERVVFRQAGLR *F .: .: : .: .:. :: : .:. *F SWALL: DAAYKAMMEEMKSQAILVSGESGAGKTETTKQIMQYLAFVGGRTVGD--ERSVEQQVLQS *F 150 160 170 180 190 *F *F 320 330 340 350 360 370 *F /net/n QSLGAYFQLTQAVRLGNLKRFGDVVS-QYGP-KFQLDHTFT-LIIRLRHNVIKTAIRSIG *F . : : ...:: .: .::: : :.. :.. . : :. : : . :.. :. *F SWALL: NPLLEAFGNAKTVRNNNSSRFGKFVEIQFNNGKISGAAVRTYLLERSRVTQISSPERNYH *F 200 210 220 230 240 250 *F *F 380 390 400 410 420 *F /net/n LSYSRI---SPQDIAKRLMLDSAEDAEFIV-SKAIRDGVIEATLDPAQNFMRSKESTDIY *F :. . ::.: :.:: : .. ... :: .. :.: : .... ..:. :: *F SWALL: CFYQLVAGASPED-AERLKLGPPDSFHYLNQSKCVEVGAI----DDCKEYQLTREAMDIV *F 260 270 280 290 300 310 *F *F 430 440 450 460 470 480 *F /net/n S-TREPQLAFHERISFCLNLHNQSVKAMRYPPKSYGKDLESAEERREREQQDLELAKEMA *F . : : : :. . :. :.: : . :.. ...: :. . :. : :: *F SWALL: GITTEEQEAIFRTIAAVLHLGNIEFDS--------GES-DASEVSTEKSKFHLKAAAEML *F 320 330 340 350 360 *F *F 490 *F /net/n EDDEDGF *F ::. *F SWALL: MCDEQMLEKSLTTRIMKATRTESITKILNKSQATDNRDSIAKTIYAKLFDWLVNKVNKSI *F 370 380 390 400 410 420 *F *F *F *F 494 residues in 1 query sequences *F 120063421 residues in 380234 library sequences *F Tcomplib (4 proc)[version 3.2t09 December 7, 1999] *F start: Mon Dec 27 19:57:11 1999 done: Mon Dec 27 19:57:57 1999 *F Scan time: 117.760 Display time: 0.680 *F *F Function used was FASTA # *U FBrf0125015 *a Davis *b I. *t 1999.12.9 *T personal communication to FlyBase *u *F From ilan@holyrood.ed.ac.uk Thu Dec 09 11:00:22 1999 *F Date: Thu, 9 Dec 1999 11:04:30 +0000 *F To: Chihiro Yamada *F From: Ilan Davis *F Subject: Re: FlyBase Query (cy269) *F Dera Chihiro, *F The 44 copies were oligomerised in vitro. They originate from an *F unpublished RNA sequence that binds to rhodamine on a column (but not in *F vivo in flies) that was in vitro selected (SELEX). It is 44 copies of *F approx. 100bp sequence which is non-coding and originally made as DNA *F oligonucleotides. *F I hope this answers your questions. *F with best wishes *F ilan *F >Dear Dr Davis, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Wilkie et al. 1999 Current Biology 9(21) 1263-1266. *F > *F >I have a question I was hoping you could answer for me. *F > *F >hb-X44 *F > *F >In fig 4. and the supplementary material, you mention a construct X44, *F >that consists of three bcd-binding sites from a 0.75kb fragment of *F >the hb promoter driving the expression of 44 tandem non-coding *F >repeats and lacking polyadenylation and transcription termination *F >sequences. *F > *F >Could you tell me where the 44 tandem non-coding repeats came from? *F >Are they a known class of repeat (e.g. opa repeats)? Were the 44 *F >repeats found as a whole from genomic DNA, or were they oligomerised *F >in vitro? *F > *F >Thanks *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- *F ____________________________________________ *F Dr Ilan Davis *F Wellcome Institute for Cell Biology *F ICMB, King's Buildings, Edinburgh University *F Mayfield Road, Edinburgh EH9 3JR, Scotland *F Office: 0131 650 7115 Lab: 0131 650 7110 *F Fax: 0131 650 7031 *F email: ilan.davis@ed.ac.uk *F Web page: http://www.ed.ac.uk/~ilan *F ______________________________________________ # *U FBrf0125024 *a Spradling *b A. *t 1999.12.9 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Oct 07 12:46:09 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Oct 1999 12:46:09 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase with P-element paper *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:47:54 +0100 *F Content-Length: 1835 *F Dear Allan, *F I have curated your P-element paper from Genetics (Genetics 153(1): *F 135--177) for FlyBase and have several questions about some of the *F lines in the various Tables - although given the amount of data in the *F paper there are relatively few questions. *F I have tried to divide the questions into groups, and I will be sending *F each group of questions in a separate e-mail with a different subject *F line. Hopefully this will make the questions more manageable and will *F stop us getting confused - if you can reply with the same subject line *F in the e-mail that would be a great help. *F Your answers will be recorded as a personal communication from you to *F FlyBase and will be linked to the Genetics paper in FlyBase so that *F people can easily see the extra information about the lines in your *F paper. *F In the following e-mails I have used the following *F abbreviations/syntax: *F 'BFD' signifies the 'Berkeley Fly Database'. *F '' in allele designations signifies superscript. *F I will be sending the following e-mails: *F Subject: Helping FlyBase (P-element) - possible typographical errors. *F Subject: Helping FlyBase (P-element) - cytology questions. *F Subject: Helping FlyBase (P-element) - nomenclature questions. *F Subject: Helping FlyBase (P-element) - confirming the identity of alleles. *F Subject: Helping FlyBase (P-element) - separable lethals ? *F Subject: Helping FlyBase (P-element) - miscellaneous *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From gm119@gen.cam.ac.uk Thu Oct 07 12:46:22 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Oct 1999 12:46:22 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - cytology questions. *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:09 +0100 *F Content-Length: 8343 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: cytology questions. *F 1. the cytology (as given in FlyBase and the BFD) of the reserve strain *F does not match the cytology of the primary strain (I am defining 'does *F not match' as any instance where the cytology does not overlap - even *F if the cytologies are only a band apart) given in Table 4 or 5. I have *F included a table of these below. This is a problem for us because we *F do not know where to place the lethal mutation on the chromosome. *F In these cases: *F a. is the cytology of the reserve line as we have it in FlyBase and the *F BFD correct ? *F b. Which of the reserve insertions have been verified ? *F if the cytology of the reserve lines is correct it seems likely that a *F second mutation on the chromosome is what is allelic to the primary *F line (at least for the top 2 lines) as the cytology of the inserts is *F so different - in that case I will make new alleles that are not *F associated with the insertions for the reserve lines. For the others *F are the cytologies close enough to presume that they are in the same *F gene ? *F Primary | cytology of | Reserve | cytology of Reserve | *F | primary line | | in FlyBase+BFD | *F \-------------------------------------------------------------- *F l(2)k06502 | 25F3-4 | l(2)02839 | 53B1--2 | *F \-------------------------------------------------------------- *F l(2)k10617 | 27C6-8 | l(2)k11018 | 47C3--4 | *F \-------------------------------------------------------------- *F l(2)k08316 | 58E1-2 | l(2)k08134 | 59B1--2 | *F \-------------------------------------------------------------- *F l(3)02240 | 67C4-5 | l(3)rK145 | 67B4--5 | *F \-------------------------------------------------------------- *F From spradling@mail1.ciwemb.edu Mon Oct 11 23:16:29 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 11 Oct 1999 23:16:29 +0100 *F Date: 11 Oct 1999 18:15:38 -0400 *F From: 'Allan Spradling' *F Subject: Re: Helping FlyBase (P-eleme *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 11905 *F Reply to: RE>Helping FlyBase (P-element) - cytology questions. *F \-------------------------------------- *F Date: 10/7/99 7:59 AM *F To: Allan Spradling *F From: Genetics *F Received: by mail1.ciwemb.edu with SMTP;7 Oct 1999 07:48:44 -0400 *F Received: from gilly.gen.cam.ac.uk (131.111.46.170) *F by mauve.csi.cam.ac.uk with smtp (Exim 3.03 \#1) *F id 11ZC1u-00023G-00; Thu, 07 Oct 1999 12:48:14 +0100 *F Received: from gm119 by gilly.gen.cam.ac.uk with local (Exim 1.58 \#2) *F id 11ZC1p-0000uG-00; Thu, 7 Oct 1999 12:48:09 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - cytology questions. *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F Message-Id: *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:09 +0100 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: cytology questions. *F 1. the cytology (as given in FlyBase and the BFD) of the reserve strain *F does not match the cytology of the primary strain (I am defining 'does *F not match' as any instance where the cytology does not overlap - even *F if the cytologies are only a band apart) given in Table 4 or 5. I have *F included a table of these below. This is a problem for us because we *F do not know where to place the lethal mutation on the chromosome. *F Welcome to the field of cytology. The database reports the data as it was *F actually done. Since allelism was not determined until later, it represents *F completely unbiased data on cytology. At least a few of the differences are *F probably real, since P elements mutating the same gene can be separated by *F more than 50kb if the gene is large, and could reside truly in different *F bands. Your problem is just to report the data. I note many entries in *F Flybase genes where some of the cytology is contradictory. Users understand *F that there are errors in localizing breakpoints and in situ sites. It is *F worthwhile to look at the cytology of all the P alleles of a gene when there *F are more than 2. Often, only one is an outlier. We have redone some of these *F when the discrepancy was large and corrected the data in earlier versions of *F the database. Other differences we consider too minor to worry about. *F In these cases: *F a. is the cytology of the reserve line as we have it in FlyBase and the *F BFD correct ? *F Usually, but you do not have the latest version of the P element database and *F a few corrections were made. *F b. Which of the reserve insertions have been verified ? *F All reserve insertions of lines with a phenotype are verified. *F if the cytology of the reserve lines is correct it seems likely that a *F second mutation on the chromosome is what is allelic to the primary *F line (at least for the top 2 lines) as the cytology of the inserts is *F so different - in that case I will make new alleles that are not *F associated with the insertions for the reserve lines. For the others *F are the cytologies close enough to presume that they are in the same *F gene ? *F Primary | cytology of | Reserve | cytology of Reserve | *F | primary line | | in FlyBase+BFD | *F \-------------------------------------------------------------- *F l(2)k06502 | 25F3-4 | l(2)02839 | 53B1--2 (error) | l(2)02839 is *F not 53B1-2; this was a mixup with 02836 which is at that site *F \-------------------------------------------------------------- *F l(2)k10617 | 27C6-8 | l(2)k11018 | 47C3--4 (error) | l(2)k11018 is *F at 27D1-2 *F \-------------------------------------------------------------- *F l(2)k08316 | 58E1-2 | l(2)k08134 | 59B1--2 | one of two *F must be wrong, not resolved *F \-------------------------------------------------------------- *F l(3)02240 | 67C4-5 | l(3)rK145 | 67B4--5 | not certain *F that rK145 is actually an allele of l(3)02240; retained in reserve due to *F possible geentic interaction; someone studying this locus will have to figure *F this out- *F \-------------------------------------------------------------- *F From gm119@gen.cam.ac.uk Thu Oct 07 12:46:36 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Oct 1999 12:46:36 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - confirming the identity of alleles. *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:23 +0100 *F Content-Length: 1818 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: confirming the identity of alleles. *F 1. fs(2)10089 is presumably either hts1 or hts2 as you give the *F reference FBrf0056130 == Yue and Spradling, 1992, Genes Dev. 6: *F 2443--2454 for this line and both hts1 and hts2 are described in *F this reference. Do you know which allele fs(2)10089 corresponds to ? *F From spradling@mail1.ciwemb.edu Thu Oct 21 15:56:18 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 21 Oct 1999 15:56:18 +0100 *F Date: 21 Oct 1999 10:56:38 -0400 *F From: 'Allan Spradling' *F Subject: Re: Helping FlyBase (P-eleme *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 3275 *F Reply to: RE>Helping FlyBase (P-element) - confirming the identity *F of... *F \-------------------------------------- *F Date: 10/7/99 7:59 AM *F To: Allan Spradling *F From: Genetics *F Received: by mail1.ciwemb.edu with SMTP;7 Oct 1999 07:48:58 -0400 *F Received: from gilly.gen.cam.ac.uk (131.111.46.170) *F by mauve.csi.cam.ac.uk with smtp (Exim 3.03 \#1) *F id 11ZC28-00023Q-00; Thu, 07 Oct 1999 12:48:28 +0100 *F Received: from gm119 by gilly.gen.cam.ac.uk with local (Exim 1.58 \#2) *F id 11ZC23-0000uK-00; Thu, 7 Oct 1999 12:48:23 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - confirming the identity of alleles. *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F Message-Id: *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:23 +0100 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: confirming the identity of alleles. *F 1. fs(2)10089 is presumably either hts1 or hts2 as you give the *F reference FBrf0056130 == Yue and Spradling, 1992, Genes Dev. 6: *F 2443--2454 for this line and both hts1 and hts2 are described in *F this reference. Do you know which allele fs(2)10089 corresponds to ? *F Yes. fs(2)10089 is hts2 and is from the Karpen and Spradling, 1992 screen; *F hts1 was recovered from the from a small pilot screen the preceeded the K *F and S screen- its original name was fs(2)PZ276. *F From gm119@gen.cam.ac.uk Thu Oct 07 12:46:47 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Oct 1999 12:46:47 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - miscellaneous *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:34 +0100 *F Content-Length: 1804 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: miscellaneous *F l(2)01528 and l(2)05287. *F we currently have two lethals assigned to the 'l(2)01528' chromosome - *F one which we think is allelic to l(2)05287 and one which is not - which *F we call l(2)39Ea01528. These have been created because of problems *F with the complementation data in the original P-element file we got *F from Berkeley. Please could you send me all the information you have *F about the following 4 lines in terms of their complementation with *F regard to each other, complementation with deficiencies, existence of *F secondary lethals etc., so that I can sort out what to do with these *F lines. The lines are: *F l(2)01528 *F l(2)05287 *F l(2)k16804B *F l(2)k08613 *F From spradling@mail1.ciwemb.edu Fri Oct 29 19:12:44 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 29 Oct 1999 19:12:44 +0100 *F Date: 29 Oct 1999 14:13:01 -0400 *F From: 'Allan Spradling' *F Subject: Re: Helping FlyBase (P-eleme *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 4751 *F Reply to: RE>Helping FlyBase (P-element) - miscellaneous *F >Subject: miscellaneous *F l(2)01528 and l(2)05287. *F we currently have two lethals assigned to the 'l(2)01528' chromosome - *F one which we think is allelic to l(2)05287 and one which is not - which *F we call l(2)39Ea01528. These have been created because of problems *F with the complementation data in the original P-element file we got *F from Berkeley. Please could you send me all the information you have *F about the following 4 lines in terms of their complementation with *F regard to each other, complementation with deficiencies, existence of *F secondary lethals etc., so that I can sort out what to do with these *F lines. The lines are: *F l(2)01528 *F l(2)05287 *F l(2)k16804B *F l(2)k08613 *F I don't see any problem with the complementation data. There are two separate *F complementation groups: *F l(2)01528 includes: l(2)k13715 , l(2)k08922, l(2)k08613 *F and *F l(2)05287 includes: l(2)k16804b *F we have no evidence of any overlap between these sets of alleles: l(2)05287 *F noncomplements only l(2)k16804b and complements l(2)01528 *F l(2)01528 only noncomplements members of its complementation group; both *F complement various deficiencies. *F From gm119@gen.cam.ac.uk Thu Oct 07 12:46:44 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Oct 1999 12:46:44 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - separable lethals ? *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:29 +0100 *F Content-Length: 4476 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: separable lethals ? *F This e-mail is about lines whose prefix (e.g. 'v(2)' ) in Tables 4 and *F 5 differs from what we have in FlyBase, so there may be separable *F second site lethals on the chromosome. *F 1. Lines which have the prefix 'n(2)' or 'n(3)' in Tables 4 and 5, but *F are currently listed as 'l(2)' or 'l(3)' in FlyBase. For each of these *F lines could you please confirm whether the original line was lethal *F when isolated i.e. is there a secondary lethal mutation on the *F chromsome which is separable from the insertion. Any information you *F have about the position of these secondary lethals on the chromosome *F would also be very useful. The lines are: *F n(2)k04512 *F n(2)05337 *F n(2)06253 *F n(2)k04810 *F n(2)k07110 *F n(2)k07332 *F n(2)k09217 *F n(2)k13807 *F n(2)k17003 *F n(2)k07245 *F n(3)s2681 *F n(3)01949 *F n(3)03884 *F n(2)k09033 *F n(2)k07236 *F reserve lines: *F n(2)k11702 *F n(2)k09303 *F n(2)k10209 *F 2. lines which are 'v(2)' or 'v(3)' in Tables 4 and 5 but currently *F listed as lethals in FlyBase. Please can you confirm for each line *F whether the original line was lethal when isolated i.e. is there a *F secondary lethal mutation on the chromsome which is separable from the *F insertion which is causing the visible phenotype. Again, any *F information on the location of the secondary lethal would be helpful. *F v(2)k09107 *F v(2)rG232 *F v(2)k06408 *F v(2)k03514 *F v(2)k16105 *F v(2)k15606 *F v(3)03847 *F Reserve: *F v(2)k11209 *F v(2)k15819 *F 3. n(2)09967 - this is given the prefix n(2) in Table 4 but is listed *F as ms(2) in FlyBase. Please could you confirm whether the original *F line was male sterile when isolated i.e. is there a secondary male *F sterile mutation on the chromosome separable from the insertion. *F 4. n(3)05241 - we have this as an allele of Hsromega (FBgn0001234) *F which is stated to be semilethal. *F Do you have any evidence that the insertion is in Hsromega - the reason *F we have it as an allele of Hsromega is that in the original data we got *F from Berkeley for the P-element project it states that the insert is in *F Hsromega, is this still true (as in Table 5 you do not say that *F n(3)05241 is in Hsromega) ? *F Does the semi-lethality of the chromosome map to Hsromega or elsewhere *F \- I think the latter given that you have given it an 'n(3)' designation *F and the P-element maps to the same place as Hsromega, and there is also *F a line in the BFD ' the semilethality maps elsewhere and can be *F recombined off (Pardue Aug 1998) '. Any info on the location of the *F semi-lethality (if separate) would be great. *F 5. ms(3)08724 - we have l(3)08724 in FlyBase. Please could you confirm *F whether there is a secondary lethal on the chromosome which is separate *F from the male sterility caused by the insertion. *F 6. lines which are 'fs(2)' or 'fs(3)' in Tables 4 and 5 but currently *F listed as lethals in FlyBase. Please can you confirm for each line *F whether the original line was lethal when isolated i.e. is there a *F secondary lethal mutation on the chromsome which is separable from the *F insertion which is causing the female sterility phenotype. Again, any *F information on the location of the secondary lethal would be helpful. *F fs(2)k09833 *F fs(2)k10816 *F 7. v(2)rJ571. The insertion is in osp. In FlyBase we have that this *F allele is semi-lethal. Is there a separable semi-lethal on the *F chromosome ? *F 8. n(2)k10237 *F is there a secondary lethal on this chromosome (as above) ? *F The reserve line for this is stated to be 'l(2)k16510'. *F What is the basis of the statement of allelism of n(2)k10237 and *F l(2)k16510 given that n(2)k10237 has no phenotype ? *F Has l(2)k16510 been verified (i.e. does the insert cause the lethality) *F or is there a secondary lethal on the chromosome ? *F From spradling@mail1.ciwemb.edu Wed Dec 08 23:16:19 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 8 Dec 1999 23:16:19 +0000 *F Date: 8 Dec 1999 18:13:57 -0500 *F From: 'Allan Spradling' *F Subject: Re: Helping FlyBase (P-eleme *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 4655 *F Reply to: RE>Helping FlyBase (P-element) - separable lethals ? *F Lines indicated below as 'l' were changed from l(2) or l(3) to n(2) or n(3) *F when complementation studies with deficiencies or other P element lines *F indicated that the P element had no associated phenotype. We did not attempt *F to map the associated lethal(s) on the original chromosome and consider them *F irrelevant. If we happen to cross off the background lethality we will *F substitute the clean stock, just as one would for any other stock that arrived *F with a background mutation on it. I do not guarantee that any of these stocks *F still have a background lethal on them. The lines were isolated because of *F their P elements, not because of the lethals. It should be noted that the *F indicated stock may contain a background mutation on the P element-bearing *F chromosome. All were saved because molecular analysis indicates that the P *F elements disrupt an interesting transcript or open-reading frame. *F n(2)k04512 l *F n(2)05337 l *F n(2)06253 l *F n(2)k04810 l *F n(2)k07110 l *F n(2)k07332 l *F n(2)k09217 l *F n(2)k13807 l *F n(2)k17003 l *F n(2)k07245 l *F n(3)s2681 l *F n(3)01949 l designation of n is based on placement of B52 to left of *F insertion by cytology *F n(3)03884 l *F n(2)k09033 l *F n(2)k07236 l ; should probably be v(2), since there is a very weak eye *F phenotype over Df(2R)Jp1 *F reserve lines: *F n(2)k11702 l ; note, not tested for sterility over Df, so n designation *F provisional *F n(2)k09303 l *F n(2)k10209 l *F 2. lines which are 'v(2)' or 'v(3)' in Tables 4 and 5 but currently *F listed as lethals in FlyBase. Please can you confirm for each line *F whether the original line was lethal when isolated i.e. is there a *F secondary lethal mutation on the chromsome which is separable from the *F insertion which is causing the visible phenotype. Again, any *F information on the location of the secondary lethal would be helpful. *F v(2)k09107 l *F v(2)rG232 l *F v(2)k06408 l *F v(2)k03514 l my record says this should be n(2) *F v(2)k16105 l *F v(2)k15606 l *F v(3)03847 l *F Reserve: *F v(2)k11209 l *F v(2)k15819 l *F 3. n(2)09967 - this is given the prefix n(2) in Table 4 but is listed *F as ms(2) in FlyBase. Please could you confirm whether the original *F line was male sterile when isolated i.e. is there a secondary male *F sterile mutation on the chromosome separable from the insertion. *F Castrillon et al. stated was allelic to ms(2)42D, but was not male sterile *F over a Df for region; I did not test to see if both lines had the same *F background male sterile *F 4. n(3)05241 - we have this as an allele of Hsromega (FBgn0001234) *F which is stated to be semilethal. *F Do you have any evidence that the insertion is in Hsromega - the reason *F we have it as an allele of Hsromega is that in the original data we got *F from Berkeley for the P-element project it states that the insert is in *F Hsromega, is this still true (as in Table 5 you do not say that *F n(3)05241 is in Hsromega) ? *F No. Insertion is located upsream from hsr-omega and the pardue lab did not *F find that the insertion disrupted the gene; *F Does the semi-lethality of the chromosome map to Hsromega or elsewhere *F \- I think the latter given that you have given it an 'n(3)' designation *F and the P-element maps to the same place as Hsromega, and there is also *F a line in the BFD ' the semilethality maps elsewhere and can be *F recombined off (Pardue Aug 1998) '. Any info on the location of the *F semi-lethality (if separate) would be great. *F Yes, our data agrees with Pardue. *F 5. ms(3)08724 - we have l(3)08724 in FlyBase. Please could you confirm *F whether there is a secondary lethal on the chromosome which is separate *F from the male sterility caused by the insertion. *F I have no record of any lethality associated with this stock. *F 6. lines which are 'fs(2)' or 'fs(3)' in Tables 4 and 5 but currently *F listed as lethals in FlyBase. Please can you confirm for each line *F whether the original line was lethal when isolated i.e. is there a *F secondary lethal mutation on the chromsome which is separable from the *F insertion which is causing the female sterility phenotype. Again, any *F information on the location of the secondary lethal would be helpful. *F fs(2)k09833 l *F fs(2)k10816 l *F 7. v(2)rJ571. The insertion is in osp. In FlyBase we have that this *F allele is semi-lethal. Is there a separable semi-lethal on the *F chromosome ? *F It was semi-lethal in our tests; I have no evidence that the semi-lethality is *F separable. Calling a line v(2) does not address the question of *F semilethality. *F 8. n(2)k10237 *F is there a secondary lethal on this chromosome (as above) ? Yes *F The reserve line for this is stated to be 'l(2)k16510'. *F What is the basis of the statement of allelism of n(2)k10237 and *F l(2)k16510 given that n(2)k10237 has no phenotype ? *F Simply the fact that the P elements in these lines are inserted 7bp apart. *F Obvioously, they are not allelic in a strict genetic sense. However, we need *F some way to indicate this molecular definition of allelism. Many such line *F pairs will eventually be found to share a subtle phenotype, such as an effect *F on the production of some transcript. *F Has l(2)k16510 been verified (i.e. does the insert cause the lethality) *F or is there a secondary lethal on the chromosome ? Both lines must have *F secondary lethals, otherwise their lethality would not complement. *F From gm119@gen.cam.ac.uk Thu Oct 07 12:46:15 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Oct 1999 12:46:15 +0100 *F To: spradling@mail1.ciwemb.edu *F Subject: Helping FlyBase (P-element) - possible typographical errors. *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 7 Oct 1999 12:48:02 +0100 *F Content-Length: 3042 *F Re: P-element paper (Genetics 153(1): 135--177) *F Subject: possible typographical errors. *F 1. in the entry for the l(3)05014 line, the deficiency '29' (Df(3L)Cat) *F appears in both the Non-comp and Comp columns of the Table - which is *F correct ? *F 2. in the entry for the l(3)06743 line, the deficiency '64' *F (Df(3R)01215) appears in both the Non-comp and Comp columns of the *F Table - which is correct ? *F 3. in the entry for the l(3)07207 line, the deficiency '58' *F (Df(3R)crb87-4) appears in both the Non-comp and Comp columns of the *F Table - which is correct ? *F 4. for line l(3)01688, the same designation is in the reserve column. *F Could you tell me what the reserve line is for l(3)01688. *F From spradling@mail1.ciwemb.edu Thu Dec 09 15:33:09 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 9 Dec 1999 15:33:09 +0000 *F Date: 9 Dec 1999 10:30:45 -0500 *F From: 'Allan Spradling' *F Subject: Re: Helping FlyBase (P-eleme *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 6231 *F Reply to: RE>Helping FlyBase (P-element) *F Subject: possible typographical errors. *F 1. in the entry for the l(3)05014 line, the deficiency '29' (Df(3L)Cat) *F appears in both the Non-comp and Comp columns of the Table - which is *F correct ? *F Non-comp is correct. The other entry was from a much earlier experiment with *F a Df(3L)Cat stock that we now presume was bad. This should not have been in *F the comp table *F 2. in the entry for the l(3)06743 line, the deficiency '64' *F (Df(3R)01215) appears in both the Non-comp and Comp columns of the *F Table - which is correct ? *F We ment to only have the non-comp entry. However, the line is only *F semi-lethal and it is a difficult call. This might be a leadky allele of *F l(3)01235. *F 3. in the entry for the l(3)07207 line, the deficiency '58' *F (Df(3R)crb87-4) appears in both the Non-comp and Comp columns of the *F Table - which is correct ? *F non-comp is correct. *F 4. for line l(3)01688, the same designation is in the reserve column. *F Could you tell me what the reserve line is for l(3)01688. *F reserve line is fs(3)02003 *F From ma11@gen.cam.ac.uk Thu Nov 25 19:00:03 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 Nov 1999 19:00:03 +0000 *F To: spradling@mail1.ciwemb.edu *F Subject: Help Michael Please *F Cc: ma11@gen.cam.ac.uk, ag24@gen.cam.ac.uk, *F gm119@gen.cam.ac.uk, gerry@fruitfly.berkeley.edu *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Thu, 25 Nov 1999 19:02:13 +0000 *F Content-Length: 465 *F Allan *F I have just finished annotating your new genes in Table 7 and *F putting in the correct database cross-references to 'homologs' and GO. *F There are several accession numbers in the right hand column which must be wrong *F \- they are neither GenBank nor SwissProt accession numbers. Can you help *F determining what they should have been please ? *F Michael *F =========================== *F Aats-ile I59314 *F Dhh1 1431254 *F Hrr25 1370424 *F Msp1 1323004 *F Zfrp8 should be: U10903 *F >From spradling@mail1.ciwemb.edu Sun Nov 28 16:15:49 1999 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Sun, 28 Nov 1999 16:15:49 +0000 *F Date: 26 Nov 1999 16:11:43 -0500 *F From: 'Allan Spradling' *F Subject: Re: Help Michael Please *F To: 'Genetics' *F X-Mailer: Mail*Link SMTP-QM 3.0.2 *F Content-Length: 5587 *F Reply to: RE>Help Michael Please *F Hi Michael, *F I presume that these are protein database numbers, because these matches were *F found by BLASTX type searches. Here is what I could come up with from the *F highly truncated BLAST output records that were saved from the searches: *F =========================== *F >Aats-ile I59314 *F sp|P41252|SYI_HUMAN ISOLEUCYL-TRNA SYNTHETASE, CYTOPLASMIC (ISOLEUCINE--TRNA *F LIGASE) (ILERS) pir||I59314 Isoleucyl tRNA Synthetase - human gnl|PID|d1006382 *F (D28473) isoleucyl-tRNA synthetase Homo sapiens Length = 1266 *F >Dhh1 1431254 *F LOCUS SCRNAH 1824 bp DNA PLN 17-AUG-1993 *F DEFINITION S.cerevisiae gene for RNA-helicase. *F ACCESSION X66057 *F NID g4352 *F VERSION X66057.1 GI:4352 *F KEYWORDS helicase. *F SOURCE baker's yeast. *F ORGANISM Saccharomyces cerevisiae *F Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; *F Saccharomycetaceae; Saccharomyces. *F REFERENCE 1 (bases 1 to 1824) *F AUTHORS Strahl-Bolsinger,S. *F TITLE Direct Submission *F JOURNAL Submitted (13-MAY-1992) S. Strahl-Bolsinger, Universitaet *F Regensburg, Lehrstuhl fuer Zellbiologie und Pflanzen, *F Universitaetsstrasse 31, Postfach 397, 8400 Regensburg, FRG *F REFERENCE 2 (bases 1 to 1824) *F AUTHORS Strahl-Bolsinger,S. and Tanner,W. *F TITLE A yeast gene encoding a putative RNA helicase of the 'DEAD'-box *F family *F JOURNAL Yeast 9 (4), 429-432 (1993) *F MEDLINE 93289822 *F FEATURES Location/Qualifiers *F source 1..1824 *F /organism='Saccharomyces cerevisiae' *F /db_xref='taxon:4932' *F /clone_lib='lambda gt11 genomic' *F gene 198..1718 *F /gene='DHH1' *F CDS 198..1718 *F /gene='DHH1' *F /codon_start=1 *F /product='RNA-helicase of the DEAD-BOX family' *F /protein_id='CAA46853.1' *F /db_xref='PID:g4353' *F /db_xref='GI:4353' *F /db_xref='SWISS-PROT:P39517' *F /translation='MGSINNNFNTNNNSNTDLDRDWKTALNIPKKDTRPQTDDVLNTK *F GNTFEDFYLKRELLMGIFEAGFEKPSPIQEEAIPVAITGRDILARAKNGTGKTAAFVI *F PTLEKVKPKLNKIQALIMVPTRELALQTSQVVRTLGKHCGISCMVTTGGTNLRDDILR *F LNETVHILVGTPGRVLDLASRKVADLSDCSLFIMDEADKMLSRDFKTIIEQILSFLPP *F THQSLLFSATFPLTVKEFMVKHLHKPYEINLMEELTLKGITQYYAFVEERQKLHCLNT *F LFSKLQINQAIIFCNSTNRVELLAKKITDLGYSCYYSHARMKQQERNKVFHEFRQGKV *F RTLVCSDLLTRGIDIQAVNVVINFDFPKTAETYLHRIGRSGRFGHLGLAINLINWNDR *F FNLYKIEQELGTEIAAIPATIDKSLYVAENDETVPVPFPIEQQSYHQQAIPQQQLPSQ *F QQFAIPPQQHHPQFMVPPSHQQQQAYPPPQMPSQQGYPPQQEHFMAMPPGQSQPQY' *F BASE COUNT 598 a 362 c 321 g 543 t *F >Hrr25 1370424 *F a search on the EST (LD08007) yields matches to the amino terminus of many *F CK-1 related genes (see below). In a previous search, Hrr25 gave the best *F match, but I don't see it now and it seems likely that the gene name should be *F something more general, such as 'CKI-related' *F emb|CAB60309.1| (AL032656) similar to Eukaryotic protein ki... 140 2e-32 *F sp|Q62763|KC13_RAT CASEIN KINASE I, GAMMA 3 ISOFORM (CKI-GA... 140 2e-32 *F sp|Q62761|KC11_RAT CASEIN KINASE I, GAMMA 1 ISOFORM (CKI-GA... 140 2e-32 *F ref|NP_004375.1|PCSNK1G3| casein kinase 1, gamma 3 >gi|4590... 139 5e-32 *F gb|AAD26526.1|AF049090_1 (AF049090) casein kinase I gamma 3... 139 5e-32 *F sp|P35509|KC13_BOVIN CASEIN KINASE I, GAMMA 3 ISOFORM (CKI-... 138 1e-31 *F ref|NP_001310.1|PCSNK1G2| casein kinase 1, gamma 2 >gi|3024... 135 5e-31 *F sp|Q62762|KC12_RAT CASEIN KINASE I, GAMMA 2 ISOFORM (CKI-GA... 127 2e-28 *F example: *F sp|Q62763|KC13_RAT CASEIN KINASE I, GAMMA 3 ISOFORM (CKI-GAMMA 3) *F >gi|1363273|pir||C56711 casein kinase I (EC 2.7.1.) *F gamma-3 - rat >gi|854737 (U22321) casein kinase 1 gamma 3 *F isoform Rattus norvegicus *F Length = 448 *F Score = 140 bits (349), Expect = 2e-32 *F Identities = 66/89 (74%), Positives = 79/89 (88%) *F Frame = +1 *F Query: 814 KSSSNNMYSTRQSVSTTTGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMEPM 993 *F + S + +STR + S+++GVLMVGPNFRVGKKIGCGNFGELRLGKNLY NE+VAIK+EPM *F Sbjct: 17 RPSGRSGHSTRGTGSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPM 76 *F Query: 994 KSKAPQLHLEYRFYKLLGSHAEGVPEVYY 1080 *F KS+APQLHLEYRFYK LGS +G+P+VYY *F Sbjct: 77 KSRAPQLHLEYRFYKQLGS-GDGIPQVYY 104 *F Msp1 1323004 *F sp|P54815|MSP1_CAEEL MSP1 PROTEIN HOMOLOG >gi|3878242|emb|CAA93516.1| *F (Z69664) Similarity *F to Yeast MSP1 protein (TAT-binding homolog 4) *F (SW:MSP1_YEAST) Caenorhabditis elegans *F Length = 357 *F Score = 272 bits (687), Expect = 6e-72 *F Identities = 139/311 (44%), Positives = 214/311 (68%), Gaps = 15/311 (4%) *F Frame = +1 *F Query: 109 KGQIFQVLVRLSVASLITYYSVKWMMNQMDPTSKNKKKAKVLAEEQLKRLAEQEGFKLRG 288 *F + ++ V +R+ A+ +++ SV++++ +DP +++K +++ \*+L + G R *F Sbjct: 4 RNELIGVAIRVVAAAAVSFLSVRYLVKYLDPNYSVNEESK----KKVAQLFHELGID-RQ 58 *F Query: 289 QEFSDYELMIASHLVVPADITVSWADIA---------------GLDSVIQELRESVVLPI 423 *F E S++E+ IA+ V D+ W \*+I G + ++ EL++ ++LP+ *F Sbjct: 59 IELSEHEIRIATQFVGGEDVGADWDEIGRTENCFAKKKKNFTGGCEELVAELKDRIILPL 118 *F Query: 424 QHKDLFKHSKLWQAPKGVLLHGPPGCGKTLIAKATAKEAGMRFINLDVAILTDKWYGESQ 603 *F + S L P+G+LL+GPPGCGKTL+AKA A+ AG RFINL V+ LTDKWYGESQ *F Sbjct: 119 RFASQ-SGSHLLSPPRGILLYGPPGCGKTLLAKAVARAAGCRFINLQVSNLTDKWYGESQ 177 *F Query: 604 KLTSAVFSLASRIEPCIIFIDEIDSFLRSRNMNDHEATAMMKTQFMMLWDGLSTNANSTV 783 *F KL +AVFS+A + \*+P IIFIDEIDSFLR R +DHE+TAMMK QFM LWDG S++ + + *F Sbjct: 178 KLAAAVFSVAQKFQPTIIFIDEIDSFLRDRQSHDHESTAMMKAQFMTLWDGFSSSGDQ-I 236 *F Query: 784 IVMGATNRPQDLDKAIVRRMPAQFHIGLPSETQRKDILKLILQSEEVSQDVDLNRLSKLT 963 *F IVMGATNRP+D+D AI+RRM A+F + +P+ QR IL +IL++E+++ V+L +++ *F Sbjct: 237 IVMGATNRPRDVDAAILRRMTARFQVPVPNAKQRSQILNVILRNEKINNTVNLGEIAQAA 296 *F Query: 964 NGFSGSDLREMCRNASVYRMRQLITS 1041 *F G SGSDL+E+CR A + R + + S *F Sbjct: 297 EGLSGSDLKEVCRLALLARAKATVAS 322 *F Zfrp8 should be: U10903 *F this was a typo truncation, as can been seen by inspection of the table # *U FBrf0125032 *a Beaton *b A. *t 1999.12.12 *T personal communication to FlyBase *u *F >From abeaton@uclink4.berkeley.edu Fri Nov 12 01:40:49 1999 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Fri, 12 Nov 1999 01:40:49 +0000 *F Mime-Version: 1.0 *F X-mailer: Eudora Pro 2.1.4 *F Date: Thu, 11 Nov 1999 18:49:35 -0700 *F To: Aubrey de Grey *F From: abeaton@uclink4.berkeley.edu (Amy Beaton) *F Subject: Re: Fwd: Re: Action Items Progress Report *F Aubrey, *F I do have the files that list the alleles of the lines in the P-element *F paper. *F Amy *F fs(2)01313 2 l(2)k10816 *F fs(2)01355 3 fs(2)06890, fs(2)04506 *F fs(2)06034 7 fs(2)04701, fs(2)04144, fs(2)01460, fs(2)04701, fs(2)05812, fs(2)02257 *F fs(2)06843 2 fs(2)P-1 *F fs(2)j13b6 4 fs(2)02403, fs(2)00115, fs(2)08109 *F l(2)00232 11 l(2)03101, l(2)06872, l(2)k00809, l(2)k05519, l(2)k05527, l(2)k05640, l(2)k05814, l(2)k09232, l(2)k05640, l(2)k10415 *F l(2)00255 20 l(2)01550, l(2)02687, l(2)03504, l(2)05479, l(2)06475, l(2)06903, l(2)07209, l(2)07692, l(2)k02603, l(2)k06713, l(2)k08813, l(2)k09018, l(2)k14715, l(2)k16604, l(2)rH541, l(2)rI043, l(2)rI482, l(2)rK499, l(2)k00612 *F l(2)00632 2 l(2)k05431 *F l(2)00642 24 l(2)00349, l(2)00482, l(2)03078, l(2)06218, l(2)06503, l(2)06686, l(2)06807, l(2)07046, l(2)k09901, l(2)10435, l(2)k00801, l(2)k01012, l(2)k02708, l(2)k03001, l(2)k03109, l(2)k03314, l(2)k07402, l(2)k10214, l(2)k11603, l(2)rE028, l(2)rH268, l(2)s3697, l(2)03089, l(2)09972 *F l(2)00681 10 l(2)01901, l(2)k06619, l(2)05282, l(2)k00115, l(2)k00812, l(2)k01106, l(2)k11904, l(2)k14904, l(2)k03617 *F l(2)00895 2 l(2)k05826 *F l(2)01068 6 l(2)07132, l(2)10681, l(2)k05808, l(2)s3547, fs(2)00233 *F l(2)01085 2 l(2)k14703 *F l(2)01094 9 l(2)07112, l(2)k02002, l(2)k02102a, l(2)k03404, l(2)k05311, l(2)k10314, l(2)k16705, l(2)rH623 *F l(2)01209 13 l(2)k00236, l(2)k09511, l(2)01018, l(2)01158, l(2)01864, l(2)06072, l(2)07776, l(2)k13802, l(2)k07138, l(2)k01701, l(2)k16502, l(2)k16721 *F l(2)01265 7 l(2)03255, l(2)04531, l(2)k00308, l(2)k10117, l(2)k13302, l(2)k14615 *F l(2)01270 5 l(2)k01108, l(2)02278, l(2)k05915, l(2)k12913 *F l(2)01272 13 l(2)06353, l(2)07162, l(2)k00228, l(2)k00804, l(2)k06108, l(2)k08008, l(2)k08918, l(2)k16718, l(2)rM692, l(2)rN167, l(2)s4402, l(2)01242 *F l(2)01275 8 l(2)04829, l(2)k06102, l(2)k07507, l(2)k10501, l(2)k17014, l(2)k07303, l(2)k17014 *F l(2)01288 2 l(2)k17029 *F l(2)01289 2 l(2)07769 *F l(2)01296 2 l(2)10530 *F l(2)01349 2 l(2)k09919 *F l(2)01351 6 l(2)k15101, l(2)02347, l(2)k00810, l(2)k08502, l(2)k13426 *F l(2)01361 4 l(2)k08224, l(2)k08105, l(2)05527 *F l(2)01410 9 l(2)05009, l(2)07463, l(2)03972, l(2)05604, l(2)k00212, l(2)02847, ms(2)01504, l(2)k04409 *F l(2)01467 9 l(2)08882, l(2)k00119, l(2)k00602, l(2)k01202, l(2)k05623, l(2)k06605, l(2)k16315, l(2)08827 *F l(2)01501 4 l(2)10432, l(2)k07717, l(2)k08204 *F l(2)01510 5 l(2)07007, l(2)k07404, l(2)s4214, l(2)k02508 *F l(2)01528 4 l(2)k08613, l(2)k08922, l(2)k13715 *F l(2)01738 7 l(2)01493, l(2)01704, l(2)01829, l(2)05084, l(2)06432, l(2)k06515 *F l(2)01863 2 l(2)rF111 *F l(2)02064 2 l(2)k04218 *F l(2)02074 2 l(2)05230 *F l(2)02132 6 l(2)08859, l(2)k05606, l(2)k14408, l(2)02121, l(2)01862 *F l(2)02353 8 l(2)05865, l(2)k00604, l(2)k01002, l(2)k06015, l(2)k07511, l(2)rK357, l(2)rP247 *F l(2)02439 11 l(2)k01501, l(2)06689, l(2)k01801, l(2)k05206, l(2)k07120, l(2)k07519, l(2)k14518, l(2)k14610, l(2)k09234 *F l(2)02448 9 l(2)02697, l(2)06652, l(2)k00501, l(2)k00704, l(2)k05313, l(2)k06130, l(2)k10504, l(2)s1534 *F l(2)02496 4 l(2)s5286, l(2)k06009, l(2)k09822 *F l(2)02502 3 l(2)rN498, l(2)rQ348 *F l(2)02516 2 l(2)k14026 *F l(2)02535 2 l(2)05096 *F l(2)02647 6 l(2)k02814, l(2)10280, l(2)k10413, l(2)k16203, l(2)rF680 *F l(2)02657 6 l(2)04894, l(2)03324, l(2)rO216, l(2)rQ639, l(2)rO505 *F l(2)02833 5 l(2)k11803, l(2)k09029, l(2)k09512, l(2)k13101 *F l(2)02836 3 l(2)03875, l(2)k14103 *F l(2)02957 2 l(2)k15609 *F l(2)02970 9 l(2)k11035, l(2)00628, l(2)05029, ms(2)00331, l(2)08253, l(2)k09828, l(2)k08507, l(2)03907 *F l(2)03021 3 l(2)03474, l(2)k11240 *F l(2)03033 4 l(2)k05115, l(2)09261, l(2)05351 *F l(2)03041 3 l(2)07010, l(2)k15608 *F l(2)03235 3 l(2)s2201, l(2)neo7 *F l(2)03267 2 l(2)k07909 *F l(2)03350 4 l(2)k13601, l(2)k15612, l(2)k10325 *F l(2)03405 9 l(2)03876, l(2)05809, l(2)k00203, l(2)k01016, l(2)k07246, l(2)k07517, l(2)k12102, l(2)s3484 *F l(2)03427 2 l(2)k09008 *F l(2)03505 11 l(2)03751, l(2)04215, l(2)04615, l(2)07470, l(2)08940, l(2)k10403, l(2)rH755, l(2)s5501, l(2)k06306, l(2)04218 *F l(2)03552 5 l(2)01300, l(2)s4158, l(2)k05702, l(2)05217 *F l(2)03575 2 l(2)k168014 *F l(2)03610 2 l(2)05467 *F l(2)03775 2 l(2)k15502 *F l(2)03832 2 l(2)k05429 *F l(2)03909 6 l(2)02828, l(2)06372, l(2)k04213, l(2)k09533, l(2)k11802 *F l(2)04111 4 l(2)k13009, l(2)k13909, l(2)rN141 *F l(2)04418 8 l(2)06470, l(2)10639, l(2)k05205, l(2)k08217, l(2)k14612, l(2)s2346, l(2)k09625 *F l(2)04440 10 l(2)03432, l(2)04525, l(2)k00702, l(2)k03304, l(2)k08415, l(2)k10126, l(2)k10315, l(2)k11204, l(2)k13817 *F l(2)04454 3 l(2)k07309, l(2)rQ447 *F l(2)04493 8 l(2)k09603, l(2)10636, l(2)k01211, l(2)k04501, l(2)k06307, l(2)04841, l(2)k09930 *F l(2)04535 7 l(2)05862, l(2)k01601, l(2)k02710, l(2)k09819, l(2)k14807, l(2)04543 *F l(2)04723 3 l(2)k13421, l(2)k16610 *F l(2)04738 7 l(2)05595, l(2)k00401, l(2)k01307, l(2)k04412, l(2)k16131, l(2)rP005 *F l(2)05056 3 l(2)k07917, l(2)k07839 *F l(2)05206 6 l(2)05277, fs(2)01672, l(2)k02602, l(2)k05007, l(2)k09109 *F l(2)05287 2 l(2)k16804b *F l(2)05441 2 l(2)k11112 *F l(2)05475 8 l(2)03679, l(2)k02517, l(2)k07505, l(2)k07701, l(2)k10907, l(2)k12405, l(2)k13706 *F l(2)05486 2 l(2)01519 *F l(2)05643 3 l(2)k07513, l(2)k11110 *F l(2)05836 3 l(2)k12603, l(2)s1883 *F l(2)05847 5 l(2)07447, l(2)k05512, l(2)k10313, l(2)k16116 *F l(2)06243 8 ms(2)08318, l(2)k04604, l(2)k07602, l(2)k13638, l(2)k09402, l(2)k06338, k(2)k09030, l(2)k13510 *F l(2)06444 3 l(2)k06005, l(2)k09720 *F l(2)06496 2 l(2)k14618 *F l(2)06825 6 l(2)k08801, l(2)k16002, l(2)k01021, l(2)02418, l(2)k08216 *F l(2)06850 3 l(2)rH247, l(2)s2140 *F l(2)06860 5 l(2)k04703, l(2)rAO104, l(2)k09230, l(2)k09702 *F l(2)06949 3 l(2)k06203, l(2)rO238 *F l(2)06955 2 l(2)k01217 *F l(2)07022 2 l(2)k01109 *F l(2)07054 9 l(2)07876, l(2)k00403, l(2)k00504, l(2)k02301, l(2)k04806, l(2)k07708, l(2)k14603a, l(2)k16302 *F l(2)07056 10 l(2)k06921, l(2)k09312, l(2)k09530, l(2)k10232, l(2)k14005, l(2)k15907, l(2)k17024, l(2)rK134, l(2)05671 *F l(2)07082 14 l(2)05729, l(2)k00109, l(2)k00606, l(2)k08910, l(2)k01502, l(2)k02811, l(2)k04408, l(2)k04802, l(2)k05210, l(2)k05818, l(2)k06211, l(2)k10817, l(2)k14811 *F l(2)07214 3 l(2)k07836, l(2)k03307 *F l(2)07812 27 l(2)k13416, l(2)02532, l(2)03303, l(2)04256, l(2)05148, l(2)05841, l(2)07815, l(2)10464, l(2)k08319, l(2)k08814, l(2)s4771, l(2)k04513, l(2)k05630, l(2)k05708, l(2)k05827, l(2)k06022, l(2)k06119, l(2)k06919, l(2)s3527, l(2)k09023, l(2)k09105, l(2)rL432, l(2)k09846, l(2)k1 *F l(2)08269 6 l(2)k02703, l(2)k07401, l(2)k09715, l(2)k05415, l(2)k08919 *F l(2)08323 2 l(2)k13606 *F l(2)08492 2 l(2)k10320 *F l(2)08770 4 l(2)02302, l(2)k04808, l(2)k14404 *F l(2)08774 3 l(2)k10909, l(2)rG034 *F l(2)09437 7 l(2)10002, l(2)k16716, l(2)k13507, l(2)k06511, l(2)k05612, l(2)k08909 *F l(2)10403 2 l(2)08639 *F l(2)10424 2 l(2)k06801 *F l(2)10469 3 l(2)k03401, l(2)k10220 *F l(2)10481 2 l(2)03263 *F l(2)10523 4 l(2)k07130, l(2)k12909, l(2)k14029 *F l(2)10607 5 ms(2)06491, l(2)07878, ms(2)05158, l(2)03264 *F l(2)10608 2 l(2)k10215 *F l(2)10626 6 l(2)k02401, l(2)k03106, l(2)k13913, l(2)k15709, l(2)k13809 *F l(2)10642 5 l(2)k04411, l(2)05208, l(2)k05905, l(2)k16802 *F l(2)10685 3 l(2)k00420, l(2)k05810 *F l(2)k00103 2 l(2)k14707 *F l(2)k00116 2 l(2)k00413 *F l(2)k00202 2 l(2)k04301a *F l(2)k00206 2 l(2)k05610 *F l(2)k00237 3 l(2)k00223, l(2)k05807 *F l(2)k00405 6 l(2)k03009, l(2)k03102, l(2)k09824, l(2)k16812, l(2)k13420 *F l(2)k00705 11 l(2)k06027, l(2)k00806, l(2)k01802, l(2)k05105, l(2)k07108, l(2)k08203, l(2)k10233, l(2)k11202, l(2)k12304, l(2)k15406 *F l(2)k01215 3 l(2)k06507, l(2)k04603 *F l(2)k01403 7 l(2)k07601, l(2)k09934, l(2)k11804, l(2)k12004, l(2)k14817, l(2)03782 *F l(2)k02507 3 l(2)rH075, l(2)rH081 *F l(2)k02512 3 l(2)k07907, l(2)k11212 *F l(2)k02701 2 l(2)00053 *F l(2)k03002 2 l(2)k04601 *F l(2)k03007 2 l(2)k13104 *F l(2)k03010 4 l(2)k03405, l(2)k05405, l(2)k06601 *F l(2)k03107 2 l(2)k13811 *F l(2)k03110 3 l(2)k04002, l(2)k02203 *F l(2)k03111 2 l(2)k02003 *F l(2)k03610 2 l(2)k03703 *F l(2)k04203 2 l(2)k04910 *F l(2)k04204 2 l(2)k05803 *F l(2)k04508 3 l(2)k05408, l(2)k16309 *F l(2)k04704 2 l(2)k06704 *F l(2)k04907 2 l(2)k06618 *F l(2)k04917 5 l(2)k05435, l(2)k06011, l(2)k08406, l(2)03647, l(2)k13707 *F l(2)k05106 2 l(2)k08036 *F l(2)k05123 2 l(2)k08405 *F l(2)k05305 3 l(2)k02303, l(2)k07501 *F l(2)k05318 2 l(2)k06308 *F l(2)k05428 3 l(2)k00108, l(2)k06708 *F l(2)k05633 9 l(2)k00508, l(2)k01208, l(2)k05403, l(2)k09317, l(2)k12103, l(2)k03101, l(2)k13409, l(2)00026 *F l(2)k05644 2 l(2)k13306 *F l(2)k05815 2 l(2)k05501 *F l(2)k05901 4 l(2)k09602, l(2)k09713, l(2)k03801 *F l(2)k06028 2 l(2)k11538 *F l(2)k06109 2 l(2)k03113 *F l(2)k06113 2 l(2)k08034 *F l(2)k06210 3 l(2)k04314, l(2)k04504 *F l(2)k06502 4 l(2)02839, l(2)k14514, l(2)s5211 *F l(2)k06602 2 l(2)k12907 *F l(2)k06709 2 l(2)k13717 *F l(2)k06710 2 l(2)k07112 *F l(2)k07005 2 l(2)k08218 *F l(2)k07104 4 l(2)k04901, l(2)k05712, l(2)k07607 *F l(2)k07135 2 ms(2)04138 *F l(2)k07215 2 l(2)k16009 *F l(2)k07612 4 l(2)k07721, l(2)k13233, l(2)k13624 *F l(2)k07624 2 l(2)k07409 *F l(2)k07824 2 l(2)k16901 *F l(2)k07826 2 l(2)k08704 *F l(2)k08003 2 l(2)k16724 *F l(2)k08108 3 l(2)k00904, l(2)k07921 *F l(2)k08110 2 l(2)k11104 *F l(2)k08115 2 l(2)k15716 *F l(2)k08121 2 l(2)k16601 *F l(2)k08316 2 l(2)k08134 *F l(2)k08815 2 l(2)k14202 *F l(2)k08901 3 l(2)k14501, l(2)k01209 *F l(2)k09003 3 l(2)k16615, l(2)k09306 *F l(2)k09010 2 l(2)k08408 *F l(2)k09410 2 l(2)k06410 *F l(2)k09507 3 l(2)k10201, l(2)k15909 *F l(2)k09614 2 l(2)k15708 *F l(2)k09801 2 l(2)k15821 *F l(2)k09905 4 l(2)k06110, l(2)k07302, l(2)k14705 *F l(2)k09923 3 l(2)k09847, l(2)k09239 *F l(2)k10307 3 l(2)k10801, l(2)k01110 *F l(2)k10308 4 l(2)k01017, l(2)k09242, l(2)k09838 *F l(2)k10617 2 l(2)k11018 *F l(2)k11403 3 l(2)k12401, l(2)k07114 *F l(2)k11511 3 l(2)04643, l(2)02459 *F l(2)k12303 3 l(2)k07214, l(2)k11320 *F l(2)k12914 2 l(2)k14509 *F l(2)k13321 13 fs(2)01320, l(2)05205, ms(2)00135, ms(2)01112, ms(2)02818, ms(2)04205, ms(2)07772, l(2)k02707, ms(2)08893, fs(2)04612, fs(2)06356, fs(2)09951, fs(2)neo1 *F l(2)k13702 2 l(2)k03509 *F l(2)k14014 7 l(2)k00310, l(2)k04805, l(2)k04912, l(2)k08103, l(2)k13903, l(2)k00802 *F l(2)k14019 2 l(2)k03202 *F l(2)k14504 2 l(2)06751 *F l(2)k14710 2 l(2)k15603 *F l(2)k14804 4 l(2)rI255, l(2)rQ802, l(2)k14312 *F l(2)k15815 4 l(2)k06202, l(2)k11507, l(2)k15512 *F l(2)k16109 2 l(2)k16218 *F l(2)k16715 2 l(2)k06303 *F l(2)k16912 2 l(2)k07737 *F l(2)k17002 2 l(2)k11534 *F l(2)s1859 2 l(2)k08920 *F l(2)s1878 2 l(2)rN173 *F l(2)s5379 2 l(2)k08027 *F n(2)k07110 2 l(2)k07103 *F n(2)k10237 2 l(2)k16510 *F sl(2)01103 6 fs(2)10089, fs(2)k14523, fs(2)k06121, fs(2)PZ276, l(2)00634 *F v(2)k05224 3 l(2)k05705, l(2)k06321 *F v(2)k15606 2 v(2)k15819 *F v(2)rG232 2 v(2)k11209 *F fs(3)02024 7 fs(3)03217, fs(3)03414, fs(3)04711, l(3)j6C3, l(3)10467, l(3)s9999 *F l(3)00035 14 l(3)00823, l(3)04050, l(3)02545, l(3)s2696, l(3)neo60, l(3)06701, l(3)04244, l(3)04095, l(3)neo63, l(3)07078, l(3)07016, l(3)j2A3, l(3)s2083 *F l(3)00073 2 l(3)10098 *F l(3)00082 2 l(3)06704 *F l(3)00090 7 l(3)j9C3, l(3)06726, l(3)s3263B, l(3)L6530, l(3)04539, l(3)04662 *F l(3)00103 4 l(3)05637, l(3)10470, l(3)neo28 *F l(3)00208 3 l(3)04727, l(3)j2e11 *F l(3)00217 2 l(3)06913 *F l(3)00295 3 l(3)04295, l(3)05415 *F l(3)00305 2 l(3)01413 *F l(3)00347 12 l(3)00700, l(3)08851, l(3)03430, l(3)s5452, l(3)01459, l(3)04733, l(3)04720, l(3)j3A2, l(3)01699, l(3)04863, l(3)j14A6 *F l(3)00451 4 l(3)02038, l(3)04693, l(3)09408 *F l(3)00564 2 l(3)04680 *F l(3)00620 3 l(3)04505, l(3)06862 *F l(3)00848 3 l(3)02288, l(3)rG347 *F l(3)00864 2 l(3)rG084 *F l(3)01086 9 l(3)02279, l(3)04136, l(3)10577, l(3)rG338, l(3)s5196, l(3)j14E8, l(3)j3D2, l(3)j3D5 *F l(3)01235 10 l(3)05473, l(3)j3A1, l(3)02499, l(3)neo62, l(3)neo61, l(3)s2213, l(3)j10B9, l(3)j1D3, l(3)j1E3 *F l(3)01432 37 l(3)j4A1, l(3)00435, l(3)00469, l(3)01824, l(3)01918, l(3)03241, l(3)03258, l(3)03603, l(3)04219, l(3)04261, l(3)05124, l(3)05339, l(3)05664, l(3)06015, l(3)06130, l(3)rO320, l(3)08435, l(3)00014, l(3)06647, l(3)rJ378, l(3)rN250, l(3)rQ383, l(3)rI739, l(3)00023, l(3)rK699 *F l(3)01453 20 l(3)j6A4, l(3)07008, l(3)01278, l(3)00462, l(3)04492, l(3)j5B13, l(3)10407, l(3)01856, l(3)03803, l(3)01164, l(3)j3B7, l(3)j3B2, l(3)j6B7, l(3)03924, l(3)05810, l(3)07097, l(3)04694, l(3)rM454, l(3)04270 *F l(3)01456 2 l(3)02466 *F l(3)01462 15 l(3)06535, l(3)03527, ms(3)01295, ms(3)neo2, ms(3)neo3, ms(3)02068, ms(3)03085, ms(3)08633, ms(3)01607, ms(3)06932, ms(3)neo6, l(3)s1782, l(3)j2C8, l(3)L7012 *F l(3)01549 2 l(3)neo45 *F l(3)01658 2 l(3)j2B12 *F l(3)01673 2 l(3)06089 *F l(3)01688 10 fs(3)02003, fs(3)04806, fs(3)04277, fs(3)06897, l(3)04338, l(3)10625, l(3)rK404, fs(3)03203, fs(3)07098 *F l(3)01705 2 l(3)04743 *F l(3)01814 4 l(3)rN672, l(3)j4A5, l(3)L1520 *F l(3)02069 4 l(3)06806, l(3)j9A6, l(3)j4B8 *F l(3)02094 2 l(3)03042 *F l(3)02231 2 l(3)j6B6 *F l(3)02281 8 l(3)01895, l(3)03229, l(3)04106, l(3)03234, l(3)06519, l(3)neo22, l(3)neo21 *F l(3)02299 9 l(3)09965, l(3)10617, l(3)L4120, l(3)s2958, l(3)j13E8, l(3)s2047, l(3)s3601, l(3)L7042 *F l(3)02404 4 l(3)07310, l(3)j5C3, l(3)neo42 *F l(3)02414 6 l(3)06848, l(3)04017, l(3)j11C8, l(3)01436, l(3)s1801 *F l(3)02619 2 l(3)j2E3 *F l(3)02640 5 l(3)04808, l(3)04563, l(3)03544, l(3)j4E3 *F l(3)02667 11 l(3)00219, l(3)10556, l(3)01316, l(3)j6C7, l(3)03540, l(3)05125, l(3)05311, l(3)05420, l(3)j2A1, l(3)j7B8 *F l(3)02733 2 l(3)10619 *F l(3)02937 3 l(3)05121, l(3)rM632 *F l(3)03342 3 l(3)09312, l(3)sA3484 *F l(3)03429 3 l(3)j11B9, l(3)s3441 *F l(3)03477 7 l(3)02731, l(3)07560, l(3)03479, l(3)j1D1, l(3)j1E11, l(3)j4C7 *F l(3)03550 5 l(3)j7A4, l(3)L3929, l(3)j1C1, l(3)L4083 *F l(3)03559 2 l(3)04410 *F l(3)03675 2 l(3)03750 *F l(3)03702 3 l(3)00692, l(3)02138 *F l(3)03719 2 l(3)j3B3 *F l(3)03773 2 l(3)02312 *F l(3)03802 3 l(3)j3B4, l(3)j7A1 *F l(3)03852 6 l(3)07038, l(3)04143, l(3)j2B7, l(3)L3101, l(3)L6202 *F l(3)03881 6 l(3)05117, l(3)07870, l(3)05584, l(3)05472, l(3)j1C5 *F l(3)03921 3 l(3)05134, l(3)05561 *F l(3)03928 5 l(3)01323, l(3)rO116, l(3)j5A3, l(3)rO154 *F l(3)03946 9 l(3)04335, l(3)01558, l(3)j1E5, l(3)j3C8, l(3)neo17, l(3)01545, l(3)02461, l(3)rO545 *F l(3)03999 3 l(3)06948, l(3)06915 *F l(3)04053 2 l(3)j8B2 *F l(3)04093 3 l(3)j8B3, l(3)j6B10 *F l(3)04220 2 l(3)j11E7 *F l(3)04322 5 l(3)07237, ms(3)03970, l(3)j4E11, l(3)05592 *F l(3)04629 2 l(3)05275 *F l(3)04696 2 l(3)04712 *F l(3)05146 2 l(3)L1602 *F l(3)05151 6 l(3)06606, l(3)03980, l(3)03935, l(3)j8C3, l(3)L9254 *F l(3)05203 6 l(3)07636, l(3)05702, l(3)08705, l(3)00934, l(3)08235 *F l(3)05284 3 l(3)rJ407, l(3)j3C3 *F l(3)05309 9 l(3)02017, l(3)00844, l(3)00876, l(3)02218, l(3)05301, l(3)rL201, l(3)rP126, l(3)rJ307 *F l(3)05408 2 l(3)j1B3 *F l(3)05461 2 l(3)s1926 *F l(3)05616 3 l(3)j5B4, l(3)L2262 *F l(3)05712 2 l(3)07484 *F l(3)05745 2 l(3)06762 *F l(3)05842 3 l(3)06656, l(3)s5349 *F l(3)05845 6 l(3)03639, l(3)05959, l(3)03032, l(3)rJ472, l(3)j5E11 *F l(3)05967 3 l(3)06318, l(3)00315 *F l(3)06240 2 l(3)rH321 *F l(3)06346 2 l(3)j6C8 *F l(3)06439 5 l(3)03676, l(3)05264, l(3)05295, l(3)04746 *F l(3)06536 2 l(3)j2E5 *F l(3)06677 3 l(3)s1747, fs(3)05703 *F l(3)06737 5 l(3)j4D9, l(3)05618, l(3)j5E6, l(3)10660 *F l(3)06916 3 l(3)00857, l(3)neo52, l(3)neo50 *F l(3)06946 8 l(3)06286, l(3)03349, l(3)00817, l(3)05221, l(3)06420, l(3)06683, l(3)10640 *F l(3)07012 3 l(3)06345, l(3)06836 *F l(3)07041 22 l(3)j12E8, l(3)05538, l(3)03338, l(3)07343, l(3)04871, l(3)rH705, l(3)j5E1, l(3)00683, l(3)02093, l(3)03554, l(3)02548, l(3)10450, l(3)j11A6, l(3)j6B3, l(3)00225, l(3)07716, l(3)05870, l(3)03247, l(3)neo25, l(3)j3A6, l(3)L5242 *F l(3)07117 5 l(3)01536, l(3)j4B6, fs(3)05678, l(3)j3B6 *F l(3)07128 4 l(3)05259, l(3)00144, l(3)06934 *F l(3)07172 4 l(3)rM729, l(3)j3B1, l(3)j3C2 *F l(3)07207 2 l(3)07883 *F l(3)07217 3 l(3)02067, l(3)08223 *F l(3)07551 2 l(3)s2427 *F l(3)07825 12 l(3)03520, l(3)04877, l(3)03680, l(3)04263, l(3)03805, l(3)05484, l(3)rP229, l(3)rH719, l(3)j1B7, l(3)rQ492, l(3)rG324 *F l(3)07842 6 l(3)05138, l(3)rL069, l(3)rA028, l(3)j2E10, l(3)j2E2 *F l(3)08126 2 l(3)j2B3 *F l(3)08232 2 l(3)j2A6 *F l(3)08247 9 l(3)00045, l(3)00682, l(3)00257, l(3)01620, l(3)06531, l(3)06613, l(3)02537, l(3)rM384 *F l(3)08310 4 l(3)10503, l(3)s2466, l(3)j3E11 *F l(3)09036 3 l(3)10052, l(3)rJ903 *F l(3)10419 19 l(3)00609, l(3)03345, l(3)05126, l(3)j6E2, l(3)rK137, l(3)02526, l(3)03261, l(3)04749, l(3)rI160, l(3)rO534, l(3)j12C8, l(3)07821, l(3)03103, l(3)06866, l(3)rK204, l(3)rL433, l(3)rJ806, l(3)rH013 *F l(3)10460 2 l(3)j5A5 *F l(3)10534 3 l(3)rL424, l(3)j4B2 *F l(3)10547 2 l(3)j9E8 *F l(3)10567 2 l(3)rF264 *F l(3)j11B2 2 l(3)j3E5 *F l(3)j1C2 2 l(3)neo33 *F l(3)j1D7 2 l(3)04860 *F l(3)j2A4 2 l(3)L8700 *F l(3)j3A4 2 l(3)j7E8 *F l(3)j3B9 2 l(3)rK215 *F l(3)j3D4 2 l(3)01058 *F l(3)j4B4 2 l(3)j8E7 *F l(3)j4D1 2 l(3)j5D1 *F l(3)j5A6 4 l(3)j7C8, l(3)j7A3, l(3)j4A3 *F l(3)j5B5 2 l(3)j5D5 *F l(3)j6B12 6 l(3)j9B8, l(3)neo37, l(3)j9B9, l(3)j9C8, l(3)rI061 *F l(3)j6B8 4 l(3)j8A6, l(3)s2898, l(3)s2818 *F l(3)j6E3 2 fs(3)00048 *F l(3)j8C8 3 l(3)j13A6, l(3)j5B1 *F l(3)L2049 2 l(3)L3879 *F l(3)L4092 2 l(3)L4091 *F l(3)neo32 2 l(3)neo31 *F l(3)rI075 2 l(3)L6731 *F l(3)rJ413 3 l(3)neo56, l(3)neo57 *F l(3)rK561 2 l(3)rL537 *F l(3)s2149 2 l(3)s3582 *F ms(3)00414 2 ms(3)00713 *F ms(3)02245 2 ms(3)05090 *F ms(3)06411 2 ms(3)08366 *F ms(3)06746 2 ms(3)06840 *F ms(3)10515 2 ms(3)07446 *F n(3)05241 2 v(3)neo55 # *U FBrf0125041 *a Valcarcel *b J. *t 2000.1.3 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Dec 16 15:42:45 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 16 Dec 1999 15:42:45 +0000 *F To: juan.valcarcel@embl-heidelberg.de *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 16 Dec 1999 15:45:35 +0000 *F Content-Length: 1769 *F Dear Dr. Valcarcel, *F I am curating your paper for FlyBase: *F Merendino et al., 1999, Nature 402(6763): 838--841 *F and I have a question about one of the constructs you used. *F pRm-A(3)m (Figure 1c). *F You state that this was generated by recloning the insert of pA(3)m *F into pRmHa-3. Does this mean that it only contains the msl-2 sequences *F downstream of the Metallothionein promoter, or are GFP sequences also *F present donstream of the msl-2 sequences - as in msl2wt-GFP (which I *F think may be the same as pRm-msl-2 (Figure 1c) from Gebauer et al., *F 1998, RNA 4(2): 142--150, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F >From juan.valcarcel@embl-heidelberg.de Mon Jan 03 20:38:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 3 Jan 2000 20:38:57 +0000 *F X-Sender: jvalcarc@popserver.embl-heidelberg.de *F Mime-Version: 1.0 *F Date: Mon, 3 Jan 2000 21:39:26 +0100 *F To: Gillian Millburn (Genetics) *F From: juan.valcarcel@EMBL-Heidelberg.de (juan valcarcel) *F Subject: Re: FlyBase query *F Dear Gillian, *F Concerning your question about our recent paper: *F >pRm-A(3)m (Figure 1c). *F > *F >You state that this was generated by recloning the insert of pA(3)m *F >into pRmHa-3. Does this mean that it only contains the msl-2 sequences *F >downstream of the Metallothionein promoter, or are GFP sequences also *F >present donstream of the msl-2 sequences - as in msl2wt-GFP (which I *F >think may be the same as pRm-msl-2 (Figure 1c) from Gebauer et al., *F >1998, RNA 4(2): 142--150, *F This plasmid is identical to pRm-msl-2 (described in the previous paper as *F msl2wt-GFP) except that the sequence between the polypyrimidine tract and *F the 3' splice site of msl-2 WT has been substituted by that shown in Figure *F 1b in the Nature paper. *F Best regards, *F Juan. *F \------------------------------------------------------------------- *F Juan Valcarcel *F Gene Expression Programme *F European Molecular Biology Laboratory *F Meyerhofstrasse 1 *F 69117 Heidelberg *F Germany *F Tel. 49 6221 387 156 *F Fax 49 6221 387 518 or 306 *F email juan.valcarcel@embl-heidelberg.de # *U FBrf0125042 *a Tower *b J. *t 1999.12.23 *T personal communication to FlyBase *u *F From jtower@rcf.usc.edu Thu Dec 23 00:32:00 1999 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 23 Dec 1999 00:32:00 +0000 *F Mime-Version: 1.0 *F Date: Wed, 22 Dec 1999 16:48:16 -0800 *F To: Gillian Millburn (Genetics) *F From: John Tower *F Subject: Re: Helping FlyBase *F Dear Gillian, *F Yes you may consider this information a personal communication to *F flybase. We know PW62 chromosome is WT for cactus, chiffon and l(2)35Fe, *F but is lethal over dfRA5. We also know PW62 is lethal over dfChif64. *F Since chif64 distal breakpoint is in cactus first intron, that suggests a *F lethal mutation on PW62 proximal to cactus. However, what that might be *F and whether it might be the PW62-2 P insertion we do not know. Hope this *F helps, let me know if any Qs. Best wishes, John *F >Dear Dr. Tower, *F > *F >I have been curating your paper for FlyBase: *F > *F >Landis and Tower, 1999, Development 126(19): 4281--4293 *F > *F >and I have also been talking to John Roote about the complicated (!) *F >'PW62' chromosome to try and make sure that we have the data correctly *F >in our files. *F > *F >John said that he had written to you about the paper and showed me the *F >e-mail he'd sent you and your reply. It would be really helpful if I *F >could curate the information in your e-mail as a personal communication *F >from you to FlyBase, as it has new information and corrections of some *F >of what was in the paper. *F > *F >I have included the copy of the mail John sent me below. I would be *F >grateful if you could write and tell me whether its OK for me to curate *F >this mail as a personal communication from you to FlyBase, *F > *F >In addition, do you know whether the 'PW62-2' insertion (that is in the *F >1.8kb transcript on your map in Figure 5 of the paper) causes lethality *F >? *F > *F >FlyBase currently has a record of one of the inserts on the PW62 *F >chromosome (also called 'k08106') as causing a lethal allele of *F >'l(2)35Fg' and this lethal maps in the region between cact and chif, so *F >I am wondering whether our 'l(2)35Fg' is the same as your 'PW62-2'. *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F > *F >-------------------------------------------------------------- *F > *F >>From jr32@mole.bio.cam.ac.uk Thu Dec 09 10:24:21 1999 *F >Envelope-to: gm119@gen.cam.ac.uk *F >Delivery-date: Thu, 9 Dec 1999 10:24:21 +0000 *F >Mime-Version: 1.0 *F >Date: Thu, 9 Dec 1999 10:26:08 +0000 *F >To: Michael Ashburner , *F > 'Gillian Millburn (Genetics)' *F >From: John Roote *F >Subject: chiffon *F > *F >>Envelope-to: jr32@mole.bio.cam.ac.uk *F >>Mime-Version: 1.0 *F >>Date: Wed, 8 Dec 1999 16:36:52 -0800 *F >>To: John Roote *F >>From: John Tower *F >>Subject: Re: *F >> *F >>Hi John, *F >> *F >>>Dear John, *F >>> *F >>>Good to see your chiffon paper in Development. *F >>>I have a couple of questions: *F >>> *F >>>1) is the distal RA5 breakpoint (Fig 5A) a typo? We know that RA5 extends *F >>>far proximal of cactus - beyond BicC (into 35E). *F >> *F >>**Yes, that is a mistake - it was supposed to be off to the left with hash *F >>marks through it... *F >> *F >>> *F >>>2) how did you determine that PW62 (=k08106) was mutant for cactus? Is *F >>>this published or did you find it to be lethal or sterile with a cac- *F >>>deletion? i'm worried because our k08106 stock appears to be wildtype over *F >>>cac alleles and deletions (e.g. II30 and III18). *F >> *F >>**This is also a mistake - we find it is WT for cactus, text was supposed *F >>to say it was lethal over RA5. (chifETBE3 is also cactus plus). *F >> *F >> *F >>> *F >>>3) is the PW62-1 insert in cact coding region? *F >> *F >>the cactus intron. *F >> *F >>> *F >>>4) have you looked for transcripts between cactus and chiffon, other than *F >>>the 3 shown on the map? And do you happen to know which correspond to the *F >>>predictions in the region: DS02740.16, l(2)35Fe (=DS02740.17), DS02740.18, *F >>>DS02740.19 and DS09218.1? *F >> *F >>** We indicate one transcript between cactus and chiffon, of 1.8 kb near *F >>chiffon, contained in the trasnsformation constructs. Several genomic *F >>fragments between chiffon and cactus hybridized to approx. 1.8 kb messages *F >>in ovary RNA - these were spread over the whole region and Gary concluded *F >>there must be more than one gene between chiffon and cactus yeilding approx *F >>1.8 kb sized transcripts, but that is a far as we took it. If it would be *F >>useful I can have Gary give you more detailed info on what fragments *F >>between chiffon and cactus hybridized on Northerns. However, I know we *F >>didn't put much effort into mapping the transcripts or trying to correlate *F >>them with the potential ORFs identified by the genome project. *F >> *F >>John *F >> *F >> # *U FBrf0125043 *a Siekhaus *b D. *t 2000.1.3 *T personal communication to FlyBase *u *F From siekhaus@uclink4.berkeley.edu Mon Jan 03 19:54:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 3 Jan 2000 19:54:39 +0000 *F Mime-Version: 1.0 *F Date: Mon, 3 Jan 2000 12:03:15 -0800 *F To: flybase-help@morgan.harvard.edu *F From: Daria Siekhaus *F Subject: 97D1-2 region *F Dear Curators *F While checking out the map of genes in the 97D1-2 region on fly base, *F I noticed that there was listed a gene called PPP (prohormone *F processing protease) as well as the gene amon. PPP is just an *F earlier version (before I had characterized the mutant phenotype) of *F the amon gene which you entered from an abstract of a meeting I went *F to. Could you guys delete the PPP, to avoid confusion? *F Many thanks! *F Daria Siekhaus # *U FBrf0125046 *a Shirras *b A. *t 2000.1.5 *T personal communication to FlyBase *u *F >From a.shirras@lancaster.ac.uk Tue Jan 04 10:46:22 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 4 Jan 2000 10:46:22 +0000 *F From: 'Shirras, Alan' *F To: 'Michael Ashburner' *F Subject: RE: Help FlyBase please *F Date: Tue, 4 Jan 2000 10:49:35 -0000 *F X-Mailer: Internet Mail Service (5.5.2448.0) *F Content-Length: 1769 *F P element transformation experiments with Orct failed to rescue the lmg *F phenotype and we subsequently discovered an adjacent gene, containing the *F l(2)03424 P element insertion in its 5' untranslated region. *F The current state of affairs is summarised in the abstract of the poster at the *F Zurich meeting, a slightly updated version of which I attach below: *F Mutations in the lemming (lmg) gene lead to abnormal mitoses and apoptosis in *F imaginal cells. Over-condensed chromosomes and polyploid nuclei are observed *F in larval neuroblasts. Lagging chromosomes are observed during anaphase in *F abdominal histoblasts undergoing their second mitosis following pupariation. *F These cells appear to exit from mitosis into a G1-like binucleate state or *F undergo apoptosis directly from mitotic arrest. Widespread apoptosis is also *F observed in imaginal discs of lmg mutant larvae. *F The lmg gene was cloned by virtue of a P element insertion in the 5' *F untranslated region (l(2)03424). lmg mRNA is found uniformly in early embryos, *F becoming restricted to the central nervous system in older embryos, and *F imaginal discs in larvae. The gene is also expressed in ovarian nurse cells in *F adult females. Alternative transcripts are observed at different developmental *F stages. The predicted protein coded by the lmg gene is homologous to the *F APC11p component of the anaphase promoting complex (APC) of Saccharomyces *F cerevisae and has homologues in humans and the nematode Caenorhabditis elegans. *F These proteins belong to a larger family of small, highly conserved RING zinc *F finger-containing proteins. *F Best wishes, *F Alan *F From gm119@gen.cam.ac.uk Tue Jan 04 14:42:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 4 Jan 2000 14:42:39 +0000 *F To: a.shirras@lancaster.ac.uk *F Subject: RE: Help FlyBase please - Orct and lemming *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 4 Jan 2000 14:45:43 +0000 *F Content-Length: 1696 *F Dear Dr. Shirras, *F I am a curator working for FlyBase. Michael has passed on to me your *F e-mail concerning Orct and lmg, so that I can record the information in *F your e-mail as a personal communication from you to FlyBase, and so *F that I can correct the information in the database. *F I have some questions which arise from Figure 1. in your paper: *F Taylor et al., 1997, Gene 201(1-2): 69--74 *F At the moment lmg and Orct are in FlyBase as one gene and I want to *F make sure that I get the accession numbers and functions etc. with the *F right genes when I make the corrections in the database to make them 2 *F separate genes. *F 1. Presumably Acer (which is in Fig. 1 of the Gene paper) is not the *F lmg gene, as in your e-mail to Michael you state that the l(2)03424 *F insertion which causes the lmg phenotype is in the 5' untranslated *F region of the lmg gene ? *F 2. In Figure 1. of the Gene paper, you show 2 cDNAs - R3 (accession *F number Y12399) and 7d (accession number Y12400) which are Orct cDNAs. *F I would just like you to confirm that these are still thought to be *F Orct cDNAs and not lmg ones. Also do you still think that the 5' ends *F of these cDNAs map upstream of Acer, so that it is within an intron of *F Orct (in which case lmg must also be in an intron of Orct)? *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From a.shirras@lancaster.ac.uk Wed Jan 05 10:48:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Jan 2000 10:48:37 +0000 *F From: 'Shirras, Alan' *F To: 'Gillian Millburn' *F Subject: RE: Help FlyBase please - Orct and lemming *F Date: Wed, 5 Jan 2000 10:51:53 -0000 *F X-Mailer: Internet Mail Service (5.5.2448.0) *F Content-Length: 1215 *F > I have some questions which arise from Figure 1. in your paper: *F Dear Gillian, *F > 1. Presumably Acer (which is in Fig. 1 of the Gene paper) is not the *F > lmg gene, as in your e-mail to Michael you state that the l(2)03424 *F > insertion which causes the lmg phenotype is in the 5' untranslated *F > region of the lmg gene ? *F > *F - Correct. *F > 2. In Figure 1. of the Gene paper, you show 2 cDNAs - R3 (accession *F > number Y12399) and 7d (accession number Y12400) which are Orct cDNAs. *F > *F > I would just like you to confirm that these are still thought to be *F > Orct cDNAs and not lmg ones. Also do you still think that the 5' ends *F > of these cDNAs map upstream of Acer, so that it is within an intron of *F > Orct (in which case lmg must also be in an intron of Orct)? *F > *F Yes, R3 and 7d are still thought to be Orct cDNAs and the 5' ends map *F upstream of Acer. Both Acer and lmg lie in an intron of the R3 form of Orct, *F lmg being transcribed in the opposite direction. Thanks to the genome *F sequencing projects, we have found that the structure of the 7d transcript *F shown in the paper is wrong - what we have shown as the downstream exon of 7d *F is in fact another organic cation transporter gene. *F Hope this helps. *F Alan *F From gm119@gen.cam.ac.uk Wed Jan 05 13:35:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Jan 2000 13:35:31 +0000 *F To: a.shirras@lancaster.ac.uk *F Subject: RE: Help FlyBase please - Orct and lemming *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 5 Jan 2000 13:38:40 +0000 *F Content-Length: 861 *F Dear Alan, *F thanks for your reply, it was very helpful. I have one more question: *F you wrote: *F >Thanks to the genome *F >sequencing projects, we have found that the structure of the 7d transcript *F >shown in the paper is wrong - what we have shown as the downstream exon of 7d *F >is in fact another organic cation transporter gene. *F do you have a name for this second organic cation transporter gene, *F e.g. Orct2, so that I can include the information that this is a *F separate gene. Also, this presuambly means that the accession numbers *F Y12400 (Genbank) and O01384 (SwissProt) contain sequences belonging to *F both Orct and the second organic cation transporter gene. *F Gillian *F From a.shirras@lancaster.ac.uk Wed Jan 05 15:46:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Jan 2000 15:46:15 +0000 *F From: 'Shirras, Alan' *F To: 'Gillian Millburn' *F Subject: RE: Help FlyBase please - Orct and lemming *F Date: Wed, 5 Jan 2000 15:49:29 -0000 *F X-Mailer: Internet Mail Service (5.5.2448.0) *F Content-Length: 1128 *F Dear Gillian, *F > do you have a name for this second organic cation transporter gene, *F > e.g. Orct2, so that I can include the information that this is a *F > separate gene. *F > *F No, we have not characterised this gene and have not given it a name, *F though Orct2 would be appropriate. *F > Also, this presuambly means that the accession numbers *F > Y12400 (Genbank) and O01384 (SwissProt) contain sequences belonging to *F > both Orct and the second organic cation transporter gene. *F > *F No, it was not a hybrid cDNA so the sequences are fine. The confusion *F arose because of cross-hybridisation on Southerns. *F While you're about it, you may wish to know that there is a gene, which *F we have called anon-29Da, situated between the downstream Orct R3 exon and *F Orct2 and transcribed in the same direction as Orct. We have submitted two *F sequences of alternative transcripts, accession numbers AJ249493 and AJ249494. *F Alan # *U FBrf0125049 *a Kramer *b H. *t 1999.12.2 *T personal communication to FlyBase *u *F From leyla@morgan.harvard.edu Thu Dec 02 16:57:55 1999 *F Subject: FBrf0111660 *F To: rd120@gen.cam.ac.uk *F FBrf0111660 *F Personal Communication from Helmut Kramer *F Date: 14 Nov 1999 *F From: Helmut Kramer *F 'I agree with the following corrections concerning the 4 hook alleles in *F Table 1 of Genetics 151:675-684 (FBrf0106787): *F hk7 altered sequence position is at bp 1014, not 2014 *F hk8 altered sequence position is at bp 1191, not 1991 *F hk9 altered sequence position is at bp 1402, not 1403 *F hk16 altered sequence position is at bp 1472, not 1572' # *U FBrf0125050 *a Pak *b W. *t 1999.12.2 *T personal communication to FlyBase *u *F From wpak@bilbo.bio.purdue.edu Thu Dec 02 14:48:50 1999 *F To: 'Rachel Drysdale (Genetics)' *F Subject: RE: Helping FlyBase: inaF alleles *F Dear Rachel, *F inaF is not an allele of any other gene. It is a totally new locus. The P *F element we used for mutagenesis is P{w+ Walter} that we got from Bill *F Engels. *F Bill *F \-----Original Message----- *F From: Rachel Drysdale (Genetics) mailto:rd120@gen.cam.ac.uk *F Sent: Thursday, December 02, 1999 5:00 AM *F To: wpak@bilbo.bio.purdue.edu *F Cc: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: inaF alleles *F Dear Bill, *F I am curating your inaF paper for FlyBase: *F Proc. Natl. Acad. Sci. USA 96 (23): 13474--13479 *F and I have a question about the inaFP105p allele. You suggest (by *F your references) that this may be a BDGP allele (despite being on the *F X). Is this so? If so, please could you tell me which line it is, and *F if not which flavour of P element is the inserted transposon. *F Many thanks for your help, *F Best wishes, *F Rachel. # *U FBrf0125051 *a Boulianne *b G.L. *t 1999.12.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Dec 08 14:26:19 1999 *F To: gboul@sickkids.on.ca *F Subject: Helping FlyBase: P4 element *F Dear Gabrielle, *F I am curating your J. Neurosci. paper *F Guo et al., 1999 (J. Neurosci. 19(19): 8435--8442) *F and have a question for you. You mention (Fig 1) a P-element insertion *F that you call P4. Could you tell me a little more about this insertion *F please, such as what kind of element is inserted, and does it cause a *F mutant phenotype due to the disruption of the L15 locus? Is it one of *F the BDGP insertions? If so it will have an insertion identifier which *F would be very useful in my curation so please could you let me know *F what it is. *F Many thanks for your help, *F with best wishes, *F Rachel. *F From gboul@sickkids.on.ca Wed Dec 08 19:33:37 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: P4 element *F Dear Rachel, *F The P4 line was originally obtained from Peter Deak who is now in *F Cambridge, not BDGP. His original number for this line is 750/2 and it is a *F placw insertion. The original line is male and female sterile but it is *F not clear if the sterility is associated with the P element insertion. The *F Pelement itself is not in L15 and does not appear to disrupt the gene *F although, as aforementioned, it remains possible that the sterility could *F be associated with the Pelement insertion and affect this gene. *F Best wishes, *F Gabrielle *F Gabrielle L. Boulianne, Ph.D. *F Senior Scientist *F Program in Developmental Biology *F Hospital for Sick Children & *F Associate Professor *F Dept. Medical Genetics *F University of Toronto *F 555 University Avenue *F Toronto, Ontario *F Canada M5G 1X8 *F Ph: 416-813-8701 *F Fax: 416-813-5086 *F email: gboul@sickkids.on.ca # *U FBrf0125052 *a Bailey *b A. *t 1999.12.9 *T personal communication to FlyBase *u *F From adina@fruitfly.bdgp.berkeley.edu Wed Dec 08 18:26:34 1999 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase *F Dear Rachel, *F The sevenless-GAL4 transgene I made is constructed as follows. (I *F named it sevEP-GAL4 to distinguish it from the Dickson construct called *F sE-GAL4). A single copy of the sevenless enhancer, defined as a 1.2 kb XhoI *F fragment, was placed 5' to a 1.05 kb EcoRI/EcoRV fragment containing the *F endogenous sevenless promoter. A GAL4 cDNA (XbaI/HindIII) was placed *F immediately downstream. A poly A signal is provided by a 170 bp BamHI *F fragment. The transformation vector used is pw8, a Pw+ vector. *F UAS-lacZ experiments confirm that this driver is much more cell *F type-specific than the sE-GAL4 construct. That is, it does not appear to *F exhibit the enhancer-independent, leaky activity of the Hsp promoter in the *F sE-GAL4 construct. Since sE-GAL4 tends to yield more robust phenotypes *F than sevEP-GAL4 I think that it is usually favored unless someone has a *F particular concern about the basal, sev-independent activity of the *F construct. *F . *F . *F . *F Sincerely, *F Adina # *U FBrf0125053 *a Mueller *b J. *t 1999.12.15 *T personal communication to FlyBase *u *F From juerg.mueller@tuebingen.mpg.de Wed Dec 15 19:52:57 1999 *F To: flybase-updates@morgan.harvard.edu *F Dear flybase curator, *F we would like to put some data as personal communication into flybase: *F We recently published a paper describing the molecular lesions in the gene *F dMi-2 (listed under the name 'Pha' in flybase): Kehle et al. (1998): dMi-2, *F a Hunchback-interacting protein that functions in Polycomb repression. *F Science 282, pp. 1897-1900. *F We now know the molecular lesion of the allele dMi-21, a recessive lethal *F allele of dMi-2: a base substitution (G to A) changes the wildtype 'TGG' *F codon for Trp 794 into the termination codon 'TGA'. *F Could you please add this information to the gene report. Thanks very much! *F . *F . *F . *F Best wishes, *F Juerg *F Juerg Mueller *F MPI fuer Entwicklungsbiologie *F Spemannstr. 35/III *F 72076 Tuebingen *F GERMANY *F phone: +49 7071 601491 *F fax: +49 7071 601384 # *U FBrf0125054 *a Schaefer *b U. *c H. *d Jackle *e Y. *f He *g H. *h Bellen *i T. *j Laverty *k G. *l Rubin *t 1999.12.10 *T personal communication to FlyBase *u *F Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Subject: Goettingen lethals - set 5 *F Dated: 10 December 1999 *F *F Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of *F Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map *F the P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read *F by both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines *F is caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView. *F *F New Alleles (presumed to be caused by P{lacW} insertions): *F l(1)G0452G0452 1D *F l(1)G0234G0234 semi-lethal 2B7-10 *F l(1)G0458G0458 2E *F l(1)G0466G0466 ? -- multi-insert line: 3A, 10E-F *F l(1)G0183G0183 3A8-10 *F l(1)G0449G0449 4D4-6 *F l(1)G0106G0106 4F *F l(1)G0303G0303 5D *F l(1)G0033G0033 6A *F l(1)G0481G0481 semi-lethal 7D14-22 *F l(1)G0482G0482 9E1-4 *F l(1)G0107G0107 11D1-4 *F l(1)G0479G0479 13D-E4 *F l(1)G0476G0476 13E1-4 *F l(1)G0473G0473 14B *F l(1)G0455G0455 14E *F *F New Transposon insertions (from multi-insert lines): *F P{lacW}G0466a 3A *F P{lacW}G0466b 10E-F # *U FBrf0125055 *a Kennison *b J. *t 1999.12.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Dec 20 14:41:48 1999 *F To: rd120@gen.cam.ac.uk *F Subject: T(Y;3)ST1 breakpoint revision *F Subject: T(Y;3)ST1 breakpoint revision *F From: Kevin Cook, Bloomington Drosophila Stock Center *F In November 1999 correspondence, Jim Kennison, NIH, stated that *F Ts(YSt;3Lt)ST1 rescues Trl (70F1-2), but not dev (70D2-3). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0125056 *a Schaefer *b U. *c H. *d Jackle *e Y. *f He *g H. *h Bellen *i T. *j Laverty *k G. *l Rubin *t 1999.12.14 *T personal communication to FlyBase *u *F Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Subject: Goettingen lethals - set 6 *F Dated: 14 December 1999 *F *F Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of *F Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map the *F P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read by *F both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines is *F caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView. *F This communication includes revised insertion sites for two of the lines included in FBrf0108529 in addition to new *F alleles and transposon insertions. *F *F Alleles with updated information (presumed to be caused by P{lacW} insertions): *F Original Update *F l(1)G0090G0090 7A1-5 ? -- multi-insert line: 6F5-6, 7A1-5 *F l(1)G0092G0092 17C1-4, 17D1-4 17C-D *F *F Updated transposon insertions (from multi-insert lines): *F Original Update *F P{lacW}G0090a 6F5-6 (new) *F P{lacW}G0090b 7A1-5 (new) *F P{lacW}G0092a 17C1-4 eliminate *F P{lacW}G0092b 17D1-4 eliminate *F *F New Alleles (presumed to be caused by P{lacW} insertions): *F l(1)G0141G0141 semi-lethal 11A7-8 *F l(1)G0145G0145 semi-lethal ? -- multi-insert line: 1F, 9E3-4, 18D5-11 *F l(1)G0146G0146 semi-lethal 17E *F l(1)G0172G0172 9C1-2 *F l(1)G0227G0227 5F1-2 *F l(1)G0236G0236 semi-lethal 8F1-7 *F l(1)G0269G0269 semi-lethal 19E *F l(1)G0280G0280 ? -- multi-insert line: 1A, 3A1-4 *F l(1)G0327G0327 1B7-10 *F l(1)G0333G0333 2D1-2 *F l(1)G0364G0364 6F1-8 *F l(1)G0372G0372 7E1-6 *F l(1)G0399G0399 1B3-4 *F l(1)G0418G0418 4C7-8 *F l(1)G0435G0435 12C *F l(1)G0448G0448 2B1-4 *F l(1)G0453G0453 8D *F l(1)G0456G0456 10B8-11 *F l(1)G0462G0462 ? -- multi-insert line: 3A, 10E-F *F l(1)G0492G0492 semi-lethal 11B13-16 *F *F New Transposon insertions (from multi-insert lines): *F P{lacW}G0145a 1F *F P{lacW}G0145b 9E3-4 *F P{lacW}G0145c 18D5-11 *F P{lacW}G0280a 1A *F P{lacW}G0280b 3A1-4 *F P{lacW}G0462a 3A *F P{lacW}G0462b 10E-F # *U FBrf0125057 *a Deak *b P. *t 1999.12.22 *T personal communication to FlyBase *u *F From: P. Deak. *F Date: 1999.12.22 *F Subject: P element flanking sequence data *F >l(3)S030003/86E14-20/Pharate adult lethal P-lacW insertion/BACR24C01 *F AATCGGGCCATGAATGACCGAAGCGATGCGTACAACAAAACCGATAGCCACAGTAGTAATTAAAGGCAATAACCGAAGCG *F TGGCGCATCGTAGTTTTTGTTACGGGTTTCCGTTCATAGTAATGCCTTTTGTTGTTGCTGTCTTTCGGTCAGTTTCTGCG *F TCGTCGGCAAAAATTTTCGGTCGCTGACGTCACACCTAGGCGCCACAAAGCTCCCCCCACTACCCACTGTCATCCCCCCC *F TTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCCGGGGTTCTATGTATCCCGTTTGTTTGTATATTGCAACATGCGTGGG *F CGTTATACTCCAACTGTATCGGTGTGAGTGAGCAAGTTTGGCTGTGTGAGGTCAGTGTTGCGTTGAACTTTTCGCATTGG *F AATGATGATGTACTAACTTTTGGCGCCCACTGTTCAATATTCCCTTTATTGCGCCTATTACGACTATCTATCTCTGCCCA *F TTTTCACTTGCGCTAATTACGCCGTTGCATGAACACTGTACTCGGGACTGAATGGAATGTTAATTGGAATTTGCAATTTT *F ATTTATCCGAATGGTCATAA *F >l(3)S026316/87E1-2/BACR48D15/pic allele/L1 lethal P-lacW insertion *F TCCTGGACAGTGTGCAGTCATGTCGCATCACTACGTGGTGACGGCGCAAAAGCCGACGGCCGTTGTTGCCTGCCTAACAG *F GTAAGAACAAGAGAGCGTGTGAAAGAGAGGGGCTCCCTATGCGGTCTCCCCCGGCGAAAACAGAAAACAAGCATGTTTGC *F CGGTCGCCGGATTACACAAGCCCATGAAACAGATTTGTTTGCTTAGCACCGGCTTCTTTGGTATGCTCCTCAGGCAACTT *F CACCTCGCCCACGGATCTCAATTTGATCATTGCCCGGAACAATCAGGTGGAAATCGATCTGGTCACGCCGGAGGGGACTG *F CGTCCCCTAAAAGAGATCAACATAAATGGCACCATTGCCGTGATGCGCCACTTCCGACCGCCCGACAGCAACAAGGATTT *F GCTCTTCATACTGACGCGTCGTTACAACGTCATGATCCTCGAAGCCCCGTATGGTCAACGACGTCATCACGGTGGTTGAC *F CAAGGCCAATGGTAATGTTTCCGACTCGGTGGGCATCCCCTCCGAAAGGAAGAATTATTGCGGCCATCGATCCCAAGGCT *F AAGGTCCATC *F >l(3)S061617/87E/BACR06H18/l(3)87Eg allele/L2 lethal P-lacW insertion *F CGCTGACGAAAACTTCTAGAAACTGTGTGTATGTGTGCGTGCGAAAGAGAAGCGGTTGTTGTCGAAAGGGGGAAAGAGAG *F CGTCAGCTGCTCGCCGTATTGAAATACGAAAATTGTTCTAGAAAATCGACTAGAAAACGTCGCAGTTATTTTCGCGAACA *F CATGGCGGCGTAACGACCTTGAATCCCGGGCTCTAGCTTCCGGCAATGCCTACTATTTTCCGTTGCCCCCCAAATTACNC *F GGAAAAAAATGGGTGATGGCATCAAGTCACAGCTGTAGAAATCAATAAACAAGAAAGGGTCATATGATCGAGTGGTTTCT *F GGATTTTGAAAAATGTATTTCTACAAAAAAAAAATGGGTAACAAAATATAGGTTACTGATTTTTGTTTACAATACTAGTT *F AGAGAGGAATCACCCTTTGAACTATGAAATGTCATTGGATGATGAAACTGAA *F >l(3)S011004/87F/BACR30G22; BACR25B05/semilethal P-lacW insertion *F GCGGGAGCGAGTGCAAAAGAGAACGATATACGGCAGGTGCGCTGCGCTCTTGATGGCACCATTCTCTTAGGCGAGTGGGT *F GAGAGCGAGTGGGGAGATGATCACGATCGTATTTTCATTCATTTCCAGGTAGAGCATATCTCTCGCTTTCGCTCTCTCTC *F TCTCTCTCCGCTCTCGCTCCCTCTCTCTCGCTCTGCTCACACCCACCTGGATTTCATTCACCAAACAGCTGTTTCTGATC *F GAGTGGCCTCGTTCTGCTCACACGTGTGTGAGGATCTTGTGTGTATGTGTGCGTTGGAGGAGCTCAGGTAAGAACTATCC *F TGGAATTCCGCGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATA *F ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATT *F CAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAAGTATGAGT *F >l(3)S011046/86F/BACR03D22/CoVa allele/Prepupal lethal P-lacW insertion *F GCACGCGTCAAGTTGAAGATGCAGTGTGACCGCAATTAAATCATCAAAAAATACCGCCTGGCAGTAGCCAGCATCAATGT *F GGACCGTTGAAAAAGAAACAAGGTTTGATTTTGATTTTTTTTTTGCTTTTTTTGGGCAAGATAGAAGAAATTAAATATAA *F GGAAAATGATAAACTAACTGTGATCTTACCCGAATTTGAAATATACTGAAGCAGAAACATTTTAAATATCTCACTGTTCC *F GTGACAGCGACAGTTATAAACGTGTCCATCCCTGGAAAAGCCAGTGTTGCCAACCATCACTCAGATCTGTCATACCCGTG *F TGAAAAGTAGCAAGAACAAGAAAAGTGAGTCGAGCTGTTACTTAACCAAATTTTTGCAATTAACAAGCATTTTACTGTTT *F TTAACGGCAGCATGTTGAGCATCACGGCCCCGTAACCTGGGCAAGCGCCCTCCGCAAGCAGCCTCCGTTCGGCACATCGT *F CCGCGCCGTTGGCCGCCCGTTGCGCTGTCTGCACGGAACCCGAAGAATCGGCGGAAGANTTCGACAAGCGCTACGAAAAA *F TTACTTCACCCGTGAAGGCATCGATGGCTGGGAAGATCCGCAAGGGCATGAACGATCTGCTGGGCATGGATCTGGTGCCC *F >l(3)S144201/86F4-9/EP(3)3738 allele/viable P-lacW insertion *F CACTTGGCCATTTGTCTCTCCATTCTCCATTTTTCGCGCCTCGCTCTCAATTCTCTGCGCTCAAATCCTCTAGCAATTCT *F AATTCGTATTCTCGCCGCCTCGCTTTGAACTTGAACTTTAAATGCACAAACCATAATCGTGTATGTTATGTTGTTGCTGG *F CCGAGGGCGTGCTCTCGCACTCTGGCAAACATGGGCTCTACGAGTTTGCTATATATACGCAGCGCAATCAGTTGCGAGGC *F AGCACTCGTTCCATTTGGGCGCTCGACAATCGCCCGCTGATCAGTTTTCGACTGGCTTGCAATTAATTCGGCTCTTGACG *F AGCCCCAAAAGTGAAAGTCGCGAGTGAAAGACGTGGCAGTTTTATATTAAAGAAAAATACGAAAACGGGCAGCAGATCAA *F ACATGAACAGTACGCAAAACACGAAATGCGAAACGGCGGCAACAAGTTAATAAATTAAGAAGGCAAACGAAAAAAATCCA *F GATTCCGAGCACTGCAAAGAAATTGGCACAAATGCTTTGCTTTTATCCGTANGAAATTCGCAAAAAATGTACAAATAAAA *F CGAAAAGAAAATTGCCACTATCAAATCCCACCGTTCTTTAACTATAGTTTCCTTCTAAATCTACCTCTAATAGGCTTTGT *F CTGTGCATTCGAAAGCCGATCAGA *F >l(3)S126113/94B5-10/BACR48C17/buttonless allele/semilethal P-lacW insertion *F CACCTTATGTGACCTCATCATGGCCGCACATATTTGGGCGATATGTGGATGCCCAGTTATCGCAGATTTGTACTAAATGT *F TTGTAAATTGTAAATTGTACTATTGCACTGGGGTTGGGAAGCATAAAAGTATTAATATATCGATAGATATCGGAAAAGTA *F TTTCTACCGTTGCTTCATTTCTATCGATAGATATATTTAAACTTCTTGTATACTTATTCTAGATTAAATGACATTTATTA *F GCTGACAAAGGAAAATAAAAAGAAATATATGAATAAATCTAGCCAAACAATAACTACACAGCGAAAGGAAAGC *F >l(3)S054907/87A/BACR03D22/l(3)86Fg allele/Embryo lethal P-lacW insertion *F CACCTTATGGGCCTTCATCATGAAGCTCGACTCACTTTTCTTGTTCTTGCTACTTTTCACACGGGTATGACAGATCTGAG *F TGATGGTTGGCAACACTGGCTTTTCCAGGGATGGACACGTTTATAACTGTCGCTGTCACGGAACAGGGGAGATAT *F >l(3)S097907/86E/EP(3)0430 allele/L3 lethal P-lacW insertion *F TGCCAGGTTAAGATCAGCGCTCTTTGTGGGTGCTCTCCAATGCTCTTGGTGGGCTCTCCATTACACTAGACTCTTTGCCA *F CTCTCTTTATATCGCTCTTTCATAGCACTACTCTCGATCTCTTTTGTTTGTGGGTTATTTGTTTAGTCTTTAGTGAGACT *F ACTGATAACCAGGTTAACTCATATCTTCCTCGTTTCGCGCGACACTCTCGCAGTA *F >l(3)S036916/86E10-13/BACR48M21/Human E2K homologue/Pharate adult lethal P-lacW *F insertion *F GGCGGACTGCAGAAAATCGACGTTGCTTTGCCTTGGCCGCTAACTCTGTTTTAAATTCGAGTGCTTTGACATAAATTGAA *F AGTAGAACAACCGTCGAACAGAATTTCGCGGACCAGAGTCGCAGCAATGACGGGAATAATTTCGATCGTC *F >l(3)S000512/87b3-5/BACR30N15/svp allele/Embryo lethal P-lacW insertion *F GTCGAAACGGTCGAGTCGAGTCGGGTCGGGTCGTGTACCTTTTGCCACGAATGTTGTGCCAGTCAGGCTCTAAAAGGCCT *F TACGAGAACAGCTGAGATTTCGCTGCTCCGCATGACGCAAAGATACCGAAATTGAAAAATTTTCAAGTCTTTAAGATATT *F ATATCTCGTGTGTGCGCCGAGCGGGAAATTAATAAGCAACATCGGAAAAATTCAAAAATTCAAAAATAAACCAAGTGTTT *F TATTAGTGAAGCCAGCTGAAATGTTAACAGCATATGCAGCTTAAAAGAAAATATTAAATTTATTTTAACATAAAGAAGAA *F GAATTATAACGCGGCTGACAAATACGAATAAAAGGATACAAGTTAAAAGCTAGAGCCGAGGACTCTATCGAAACCAAAGT *F >l(3)S028813/87b10-15/BACR28I14/semilethal P-lacW insertion *F TATTGAAGCCACGAGAAACGAAAACCGGTAAAAACCGACCAGAGGCCACCAAAAGACAGCAAACATTCGAATACAAAGTC *F AGCAAACATTGACCATTTATCAGAGGCACGCATTGAACTTGAAATTTGCCGCTGCTTTTGCCAATTTCTTGCGCGAAGGG *F AATGGACATCGTGGGAGCTTACACAAGAGCGAACGAGAGCGATAGTAAGCGCTAAGAGCAAGATGGAACGAGAGTAGTTT *F TAATTTCGTTATTGTTGTGGTCCGTTATCACGTTGCAAGAAGCGTGATGCTTCACTAAGATATTACACGCTGAGAAAACT *F GGAGCGCGTTCTTAAAGTTCANATGAACTGAATGATCTGTAATTTAAACAAAACTAATAGAACTGCTATATTCAAAATTC *F GGAATGTAAATAAAAGATTCTTCTGTCTTTAA *F >l(3)S120813/87C1-3/BACR07J06/Vha55 allele/L2 lethal P-lacW insertion *F ACACTTATGGGCCTCATCATGGTTCCAGGGCAATTTTTCATCGTACTCGCAACGACTTCGCAGGCCAAAATTTTGCGTGT *F GTGTCGCTCAGTACTGTTTCGTAGCTGAAAAATTGTACGTGCTTGTAAAAGACCACGTCCGAATAGTGGCTAAAAGCTAA *F AATCGTATCTAATTGCAGTTCGAAATTTGCAGTGCACTTTCAAGGTAGAGGCGCAGAAAAAGTCTATCCAAGATGAACGC *F CCAACAAGCCCAGCGGGAACATGT *F >l(3)S146507/87C/BACR07J06/l(3)05043 allele/Pupal lethal P-lacW insertion *F ACACTTATGTGACCGCACCATGCTTCAATTCGTTGCCCGGCTTGTAAGTCAATGCTAGTGTGATAGTAGTCTTCGGAAAA *F TCCGCAAAGTATTTTTTTTTAGTATTTTTTTAATTCGAATTCATGAAACAAGAAAATTTGTTAAAAAAACGCTAACACCA *F ACATTTTCGGAAAATGTA *F >l(3)S074205/87C/BACR07J06/nucleoporin (nup) gene/semilethal P-lacW insertion *F AAGCCGGGCAACGAATTGAAGCATAAACAAACGATGTCGCTCACCGATGTCTTGGAATTGAACAAAACGGAGTTGTTCGC *F GAAGATTCGCAATGGGTTGCCCGTGGTGCAAAGGACTCAGAACCTGCTGGACTGCAAGGACGATCTGCTCTTTGCCTGGC *F ACGCGAAGGACAGCTGTCTGTTGGTTCGCAACTGGCGCTCATCGCTGGCGGCAAAGGTGAATATCCAGTTCCAGACACTG *F ATTCCATCGAGCTTGGTGAGCCTGGAGGTGGACCGCGTGCTGGCCTCCAACGAAGGCTCCCTCGTGGCACTAATGGACCG *F CCGGCTTGTTCATAATGGAACTTGCCCCGCCGCTGGGGCCCCATTGG *F >l(3)S029910/87F/BACR03J04/Pupal lethal P-lacW insertion *F GTTTACGCAATCAGCTGCGAAAAAACACGAGAATGCTCATGTGTACATAAGTGTGTCCAAGTGCCGCACAGCGCCACAGA *F TTTCGTTTATTATTAATTTTTATGAATAACCAGCGGCCGCAGAAGGTGGC *F >l(3)S101804/87E8-12/L2 lethal P-lacW insertion *F CTCTCGACTTTCTCTCACCGCTCTCTTTGGCGGTCTCTTCTTGCGCAGCAGCACCAGCAGTTAACGGTGCATGTTGAAAA *F GTTCTCACACAAACGTCGTGAAAATCGAAATCGATAAGTAAGCAACGAATTTTAGCTGCCCAGAAAAAGACCACAAATTT *F CAGTGAAAACCCAGCGATAAGAATCCCAAAAAGTACTAATCCAGCTGAAAAACAACCATCTTAACCGGCCATGTCCAAAA *F AAAGTGTTAGCCAAGTGTTTTGAATAACGTAGTTGGTGTAAATGCTTAAAAAAAATAAGCTAGTCCGGGGCCAGAGAAAT *F CGATACGATACCCCCAAAAAAAGGGGGGCTGCTGCGTGGGCTGCCCGAGTTGAAAATTTCCAGCTAAAAATAGTACTAGA *F TTTGAGCTTGAAGAAAACCCTTGATTTCCTTTGCGTTTCGATGTCAGTTATCATTGCATTTCAGTTACATAATTTTTGAG *F >l(3)S104101/87F1-8/BACR03J04/EP(3)3732 allele/Embryo lethal P-lacW insertion *F GTCTGACCCCTTTTGTGTTCTCTCTCGCTTGCGCTCGTGCGTCGGAGTGTGTTTAAGTGTGTGTGTGTGAGGTGTCGGTG *F CGACTGTACTTCAGTCATATTAATAGAATCTTCGGGCCAAAATGCATCATCACAGTCTCGGGAGACATGGAAAATTTTCA *F ACGTTTTCCCCTGCACACACAAGCACTTTCCGCAATGAAAACCACACTCCACTTAACCTACCGACCCACACATTCTCCCC *F CTCTCGCAA *F >l(3)S008131/87E5-10/l(3)05137 allele/semilethal P-lacW insertion *F CGGTACACCCATTGAAGTCGAGCGTCAACATTAAACAACTGGTACTGGTGAAGAGCTTTTTATTGACAACGCAATCACTC *F ACCAAACTTTGTATGTGACTGTGTGTATGTGAGTGGTGATGGTGGTGCTGATGGTATCTATTGGCATAAGAAATTATAAC *F ACTCGGGAATTACGGTGTAATTGTGAGCTTTGCTAATATCTGGTATCCATTCAATGGCGCATTTCCCATATGGTCATGGG *F GTAATAGTCATGAAAATGCCATGTGCAAATGACTCAAATTTTCCATTCAGAAAACATTTATGTAATTTCAAGAAAATCCG *F GGCGTGCGTCTGCCGAGACCCATTAATCATTGTACCCTAATTAACGAAACATTATTATTTTGTGCCTAATTAAATCGATT *F GCGTTGTGTTAATGCACTTTTAAGTCAAGGTTTAATTAGAATGTTTTTGTTTGCCTGCACTTTGAGTAATTAACGCTTCG *F AAACGGAAAATCTGTTGCCTAAACGGTAACTTAGCCCGTGATAAGAATCCATAGCGAAGAATGAATGCGCCAATATTTCT *F CCCACTTGTATTACCATAATGCCTAATCCTAATTAAATATTTCTCGCACTTTTCCGTGCATGTCCCACCATAACACAATT *F GTTC *F >l(3)S076201/86E9-12/EP(3)0430 allele/viable P-lacW insertion *F GGACCACCTTATGTTATTTCATCATGGTCCGACTCGTTCTGACCCGATCCGCTCTCGGGCTCCACCTTAAATACATGTCA *F CTCTCTGGGTAGGTGTTGTGCCAGGTTAAGATCAGCGCTCTTTGTGGGTGCTCTCCAATGCTCTTGGTGGGCTCTCCATT *F ACACTAGACTCTTTGCCACTCTCTTTATATCGCTCTTTCATAGCACTACTCTCGATCTCTTTTGTTTGTGGGTTATTTGT *F TTAAGTCTTTAGTGAGACTAGTGATAAGCAGGTTAACTTATATTTCCCTCGTTTNGCGCAACACTTGCATAATTAACCAA *F CGTGTTAACAAT *F >l(3)S049215/86F/Dm2615/viable P-lacW insertion *F CACTATACTATACAACATTTACAATGTGGCTACACATACGGACAGACAATCGCCTGCAATGCACATGCAGGCACATGTGT *F CTGTATAACATAATCACTAAAAATTTATTTTATTTTTATCGATTTATTTTCGCCCGCTGCGGGCAGCGTCGCCGGCGTCA *F AAGCGTCAGGGGGTATACACTCTCACTCGAGCAGCGTCAGTGGCGGTGGTGGT *F >l(3)S050116/86F/BACR11G22/EP(3)3094 allele/viable P-lacW insertion *F GGCCACACGGATGAAATTCGTCGTCATTCGTCGGAATCATTCGAACTTTGAAAATGGATCGGTAGCTGGGAAGGAAACTT *F AAAGCGAAATACGCAAAGAAAACGGCTTTTGTCCGCTATTCAGCGATTTTTTTTGTGTTGTAATCAGCAGAGGAAATTTT *F AACGACCAACTCCACCGCCACACCAGCCATCTCCAGCAGCCCCGGAAAATAAAATAGAACTAAATTAACGCCACCATCAC *F TACAACAACCATCTCACCAACAACTACAAGAGCAACAACCACAGCAACAGCACTACTGCACCAAGCCCACAAAAAAAAAG *F TTGAAAACGCAATAATCGCAATACCCAAAGAAAAAAAACAAAAAAAT *F >l(3)S051601/87C1-3/BACR02G11/Alpha-gamma sequence/viable P-lacW insertion *F ACATCTACTTCCCCCCCGCTCCTACCCGCGACCAGACTACGTTCGGCAGAAAACTCTAACGGTTGTCTGCCGCGCATCAG *F AAAAATAAAAAGAGGCGTCTGCATGGCGCGTGCCAAGCAGAAACACGAAATTTCGTTTCATTTAAATGATCTGCGACATA *F TACATACATATTACATACATATTTTCAACACTCCTTCTCAATGCAATATGTCAACCCTTACGGATAATATTTTTATCTTA *F ATTCCAAAATCAAAATACATTTTTGTTATTTTAAACCCATCCCTTGGATACACTTTTCATGTTTAAATCTATTCAAATTC *F TTGGTTAGAAACATCTGCCATCATGTTTTCTGTGGGTACATAATTAACTTCTATTATATTGTTCTTATACAATTCTCTTA *F TATAATGATATTTTATATCTATATGCTTACTACGTGCATGGAAAGTTTAATTTTTCACCAAGCACTGGGAACTCTGGTTG *F TCGCTATAAACCTTAATTGCCTGTGTTTCACCAAAACCATTTCGTTGAACAATTTCCGAAACATACCCACTTCCTTCGCT *F GCCATGGAAAATGCCACATACTCTGA *F >l(3)S108408/100E/BACR48C22/awd allele/viable P-lacW insertion *F CTCCTGATGCTCGGCACAATTTTGGCATTCTTTTCTGTAATCTCGGCGACAATGGCGGCTAACAAGGAAGAGGACTTTCA *F TCATGGTCAAGCCCGATGGCGTCCAGCGCGGGCTCGTCGGCAAGATCATCGAGCGCTTCGAGCAGAAGGGCTTCAAGCTG *F GTCGCCCTGAAGTTCACCTGGGTAAGCGGGATAATTGAATTAGGAAAGAAAATCAATAGATATAAATACTGGGAAACGGG *F TTGGCCCCACCGGGGGTT *F >l(3)S125006/87D1-5/BACR14H21/viable P-lacW insertion *F GGTGAGTGCTATTTTAAGCACCCAAGATAATTGCAACAATAAAGCAAAATAGATCACTTTTGCATACGCGCGACACAACC *F GATCTCTATGAATGCAGATTTGCGACTTCTCCAATTTCACTAGTTTAGAAACTGTGCTCGTTTCAACAAAATTGAAAAAG *F GCAAGAGTGCAATTGCAGCAGCAGGTGAGGAAGTAAGCAGCCCCCAAGGACTCACCTTAAATACCTAGAAGCGACTTTGA *F AAAACTTCATTAAGGATACATCGACAGTGCACAGTAATAAGGATTGTAATAAACTAATCTGTTGACAATGTTCCGCCTTT *F GTCTGTCCAAGTACAGATAATATCATGCACTTATGCAGCTTAATGGTCTTTT *F >l(3)S011041/87F10-15/BACR30G22/EP(3)3183 allele/viable P-lacW insertion *F GCGGGATCGAGGTGCAAAAGAGATACGAGTATACGGCAGGTGCGCTGCGCTCTTGATTGGCACCATTCTCTTAGGCGATG *F TGGGTGAGATGCGATGTGGGGAGAATGATCACGATCGT *F >l(3)S130313/87F1-3/BACR06H18/viable P-lacW insertion *F CGTCAAGCTCTCTACAACAATCAACAGTTCGCGCTCAGCCACCGAAACCGCTAACAACACGACCGCCGTTCGTTACGTGC *F TGGCTATATAACTGCATTTATAAGAAAATTCTGAAGAACGAGAAGCCTGAAGATGAGTCACAAAATCGCCGCTGTGTGCC *F TCTTGATGAGCTGCCTGATTGCCACGGCTTATAGCGCCGCTAAGGTGAGTTTCCGGCTCGGAAAAACCCTTCGCCAAACT *F AGCGAAAAATCAGCCAACGGGCCCAACTAAGCCATTACACAACAGCCAAACGTGTCAGGGGCACTCCGAAATTGCTTTTA *F CCATTTAGGTGAANACGTGATTAAGTCGAATAACTAAATATGGGGGTTAGGAAAA *F >l(3)S025616/87D6-14/BACR14H21/CtBP allele/viable P-lacW insertion *F TGAACACCTTATGTGCCCCACCATGACTAAAACCTAAAAATCCGCCGTGTTCGTATTCCGCCATTTTGAAAGCTGCGAAG *F TGGAATTTGGACGCGTTGCTGGTAAATTAATTGTGGGAAAACTGCCCAAAAAGTGCATAAAATTTGTGAAAAGTGGTCGG *F CAACGGTGCATGAGACCCGCCGCCAAGTGTTTGTCCCCGAAAATTGTGTGTGGCCGCGTTTTTGGCACGTGAATTTCTTG *F CCCAATTGTGAACTCGCCCAAAAAGAA *F >l(3)S022122/87D9-14/BACR14H21/EP(3)3352 allele/viable P-lacW insertion *F GTACAGCAATGACGCTATGCGAACAGTGTGACCGCACACGGGTCGTTCGAAAATACCTCGGTGCGCGCAGACAAACTGCA *F AGAAATTATGGTATAGTTTGAAATGTTGGTATTGCAGAATTCCGCGGAATTAATTCTTGAAGACGAAAGGGCCTCGTGAT *F ACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCG *F GAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAAACAATAACCCTGATAAATGCTCCAT *F TATTTT # *U FBrf0125058 *a O'Connor *b C. *t 2000.1.4 *T personal communication to FlyBase *u *F From bdgp-owner@fruitfly.bdgp.berkeley.edu Tue Jan 04 22:32:33 2000 *F To: bdgp@fruitfly.bdgp.berkeley.edu *F Subject: bdgp Pcmt localization information *F Dear BDGP members, *F My lab originally identified the Pcmt gene in Drosophila and we have *F been doing some cross-hybridization experiments to develop a physical *F map of the region (hopefully as a start for a P element hop). We now *F have acquired quite a bit of information that I would like to share *F with you, in the hope that it will contribute to the mapping project. *F As a result of these various cross-hybridization experiments, described *F below, I think we can place Pcmt confidently in 83A5-6. *F We performed hybridization with cosmid contig 659. We found that both *F cDNA and genomic clones for Pcmt hybridized with cosmids 80E1 and 112E7 *F (which appears to be contained within 80E1. Cosmid 80E1 cross-hybridizes *F with cosmid 189F12, although the Pcmt gene is not included in 189F12. *F We have also analyzed the position of P element insertions (using *F plasmid rescue protocols with genomic DNA) on the cosmid clones. These *F have shown that P1597 (also referred to as l(3)03644) is contained *F within 80E1 and 189F12. The P element insertions P212 (also l(3)j5E2) *F and P1664 (also l(3)05616) are present on 189F12, but not on 80E1. If *F you would like a graphical depiction of the physical distances that we *F have estimated, I would be happy to FAX or mail this to you. *F In other experiments we have sequenced the 5-flanking region of the Pcmt *F gene and found an open reading frame corresponding to EST clone CK01830 *F about 473 bp upstream from the initiation codon of the Pcmt gene. *F In my computer search of your site, I found today that Pcmt is in EST *F clot 8838 and overlaps with AC013960. Since I was unable to find this *F last item or the Pcmt on the scaffold of 3R, I thought this information *F could be useful to you. I hope that this not superfluous by now. *F As a personal favor, would you acknowledge the receipt of this message? *F Sincerely, *F Clare O'Connor # *U FBrf0125059 *a McGinnis *b W. *t 2000.1.7 *T personal communication to FlyBase *u *F From mcginnis@ucsd.edu Fri Jan 07 17:21:25 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: spen allele *F Hi Rachel, *F I went back and looked at Gellon . . indeed we didn't list poc361 *F as one of the poc (now spen) alleles that we isolated. We said that *F we isolated six alleles of poc/spen, but didn't say that their lab *F numbers were 66, 231, 247, 259, 361, and 430. We sent the 231 and *F 361 alleles of poc/spen to Staehling-Hampton, and they used them in *F their paper. *F So you are right, the spen361 we mention in the results should *F properly be called spenpoc 361. *F Best, *F Bill *F >Dear Bill, *F > *F > *F >I am curating your Development paper for FlyBase *F > *F >*x FBrf0112078 == Wiellette et al., 1999, Development 126(23): 5373--5385 *F > *F > *F >you mention an allele, spenpoc361, that you say was reported in *F > *F >*x FBrf0098246 == Gellon et al., 1997, Development 124(17): 3321--3331 *F > *F >but I don't see it there. There is a spen361-6, published in *F > *F >*x FBrf0111491 == Staehling-Hampton et al., 1999, Genetics 153(1): 275--287. *F > *F >Is this the same allele as your spenpoc361? I think, when you use *F >the symbol spen361 in the text (e.g. first paragraph of Results) that *F >you mean the spenpoc361 mentioned in Materials and Methods - but if *F >you could confirm that for me I would be grateful. *F > *F >With best wishes, *F > *F >Rachel. *F > *F >---------------------------------------------------------------------- *F >Rachel Drysdale, Ph.D. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: rd120@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- # *U FBrf0125061 *a Bloomington Drosophila Stock Center *b ?. *t 2000.1.24 *T personal communication to FlyBase *u *F *F From kcook@bio.indiana.edu Mon Jan 24 14:01:28 2000 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Mon, 24 Jan 2000 14:00:04 -0500 *F To: crosby@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{w[+mC]=UAS-crb.wt}30.12e insertion *F Mime-Version: 1.0 *F Content-Transfer-Encoding: 8bit *F X-MIME-Autoconverted: from quoted-printable to 8bit by morgan.harvard.edu id OAA20850 *F *F From: Kevin Cook, Bloomington Drosophila Stock Center *F *F Subject: P{w[+mC]=UAS-crb.wt}30.12e insertion *F *F The P{w[+mC]=UAS-crb.wt} construct and its transformation were described in *F Wodarz et al., 1995, Cell 82(1): 67--76 (FBrf0082789). The *F P{UAS-crb.wt}30.12e insertion is a homozygous viable, second chromosome *F insertion sent to the Bloomington Stock Center in October 1999 by Elisabeth *F Knust, Heinrich Heine Universität. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F # *U FBrf0125062 *a Bloomington Drosophila Stock Center *b ?. *t 2000.2.7 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 7 10:08:44 2000 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Mon, 07 Feb 2000 10:07:25 -0500 *F To: crosby@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{w[+mC]=UAS-Dl.J} and P{w[+mC]=UAS-Dl.H} insertions *F Mime-Version: 1.0 *F *F From: Kevin Cook, Bloomington Stock Center *F *F Subject: P{w[+mC]=UAS-Dl.J} and P{w[+mC]=UAS-Dl.H} insertions *F *F The following information accompanied stocks from Marc Muskavitch, Indiana *F University (2/00). *F *F Marc Haenlin isolated a homozygous viable, second chromosome insertion of *F P{UAS-Dl.H}. *F *F Tom Jacobsen isolated a homozygous viable, X chromosome insertion of *F P{UAS-Dl.J}. *F *F The insertions will be denoted P{UAS-Dl.H}MH1 and P{UAS-Dl.J}TJ1, respectively. *F *F *F *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F # *U FBrf0125063 *a Bloomington Drosophila Stock Center *b ?. *t 2000.2.7 *T personal communication to FlyBase *u *F *F Personal communication from: Bloomington Drosophila Stock Center, Indiana *F University *F Dated: 7 February 2000 *F *F Background: The chromosomal location of the GAL4 insertions listed below was *F not known when these lines were added to the collection. We have now mapped *F these insertions to a chromosome via segregation. A more specific map *F position of the insertion is not known. *F *F Information communicated: *F *F Insertion Maps to chromosome *F *F P{GawB}V85 1 *F P{GawB}5015 2 *F P{GawB}5108 2 *F P{GawB}5053A 3 *F # *U FBrf0125064 *a Bloomington Drosophila Stock Center *b ?. *t 2000.2.7 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 7 11:03:50 2000 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Mon, 07 Feb 2000 11:02:31 -0500 *F To: crosby@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{w[+mC]=UAS-Dl.DN}TJ1 insertion *F Mime-Version: 1.0 *F *F From: Kevin Cook, Bloomington Stock Center *F *F Subject: P{w[+mC]=UAS-Dl.DN} insertion *F *F The following information accompanied stocks from Marc Muskavitch, Indiana *F University (2/00). *F *F Tom Jacobsen isolated an insertion of P{UAS-Dl.DN} on a TM3, Sb[1] *F balancer. The construct and its transformation were described in Huppert *F et al. 1997, Development 124(17):3283--3291 (FBrf0098270). *F *F The insertion will be denoted P{UAS-Dl.DN}TJ1. *F *F *F *F *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F # *U FBrf0125065 *a White *b K. *t 2000.2.23 *T personal communication to FlyBase *u *F From: 'Kevin P. White' *F To: Leyla Bayraktaroglu *F Date: Mon, 21 Feb 2000 14:19:57 -0800 *F Re: EST clones in Science 286:2179-2184 paper. *F After re-BLASTing the EST sequences against the whole genome sequence, we *F determined that there are 4 clones from Figure 3B that correspond to *F projectin (bt). They are *F GM13171 (mislabeled titin), GM10074 (mislabeled C-protein), HL02324 *F (mislabeled myosin LCK), and HL05966 (labeled correctly as projectin). *F The other clone IDs currently appear to still be up-to-date with the *F identity corresponding to a known Drosophila gene (such as HL03534 *F which is troponinI up and GM05163 which is a myophilin-type molecule *F that corresponds to the gene mp20). Alternatively, they are labeled with *F the name of the closest homolog in a BLAST search (such as GM14784 which *F is closest to troponinC and HL01080 which is related to telokin and titin). # *U FBrf0125066 *a Bloomington Drosophila Stock Center *b ?. *t 2000.3.2 *T personal communication to FlyBase *u *F 29 Feb 2000 *F *F Re: Communication from Bruce Edgar to Bloomington Stock Center *F *F Subject: P{Ubi-GFP(S65T)nls insertions *F *F The following information accompanied stocks donated to the Stock Center *F (1/00) by Bruce Edgar, Fred Hutchinson Cancer Research Center. *F *F 1. P{w[+mC]=Ubi-GFP(S65T)nls} *F *F From Bruce Edgar: *F "This is a new construct we made with the NLS on GFP(S65T). It has the same *F Ubiquitin promoter as the one from O'Farrell (which is not S65T). Welcome *F Bender made the transgenics. It is marked with w[+mC], but we don't have *F the chromosome locations." *F *F 2. P{w[+mC]=Ubi-GFP(S65T)nls} *F *F Edgar sent insertions on X, 2L, 2R, 3L and 3R. They are distal to the FRT *F insertions P{neoFRT}18A, P{neoFRT}40A, P{neoFRT}42D, P{neoFRT}80B and *F P{neoFRT}82B, respectively, but have not been localized precisely. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0125067 *a Brendza *b K. *t 2000.3.2 *T personal communication to FlyBase *u *F Personal Communication to FlyBase *F Received from: Katherine Brendza *F Washington University School of Medicine *F Department of Biochemistry and Molecular Biophysics *F Subject Khc gene annotation *F Date: March 2, 2000 *F *F Query from curator: *F *F I am currently working on Khc and wanted to include the sites *F of the mutations cited in the Brendza et. al. 1999. J.B.C. *F 274(44):31506-31514 publication in the annotation. I had a bit of *F trouble matching up the stated locations of the nucleotide and amino *F acid changes with the actual sequence. My best guess for the actual *F changes are below. Could you please confirm or correct these. *F *F mutation nt change nt change aa change aa change *F paper my guess paper my guess *F *F Khc[8] C955T C948T R219@ R210@ *F *F Khc[4] C1198T C1192T T291M same *F *F Khc[2] C1078T G1071A E251K same *F *F Khc[10] T1294A A1287T T323S same *F C1396T G1389A E357K same *F *F Khc[37] C1156T G1149A D277N same *F *F Khc[36] G1234A C1227T R303C same *F *F Khc[32] G720C G720A E134K same *F *F Khc[24] C513T same Q65@ same *F *F Khc[27] C513T same Q65@ same *F *F Khc[23] C817T G810A E164K same *F *F Khc[19] G856A G849A E177K same *F *F Khc[18] G565A G558A V80I same *F *F Khc[17] C1064T C1057T S246F same *F *F *F *F Reply: *F *F I spent the morning going through Joy Yang's sequence and looking at our *F changes and your guesses are correct based on Joy's published sequence (Yang *F et.al. 1989. Cell 56:879-889). I have listed below the changes that are *F correct.. *F *F mutation nt change aa change *F *F Khc[8] C948T R210@ *F *F Khc[4] C1192T T291M *F *F Khc[2] G1071A E251K *F *F Khc[10] A1287T T323S *F G1389A E357K *F *F Khc[37] G1149A D277N *F *F Khc[36] C1227T R303C *F *F Khc[32] G720A E134K *F *F Khc[24] C513T Q65@ *F *F Khc[27] C513T Q65@ *F *F Khc[23] G810A E164K *F *F Khc[19] G849A E177K *F *F Khc[18] G558A V80I *F *F Khc[17] C1057T S246F # *U FBrf0125077 *a Bahri *b S. *t 2000.2.3 *T personal communication to FlyBase *u *F From mcbsb@mcbsgs1.imcb.nus.edu.sg Thu Feb 03 06:43:40 2000 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Thu, 3 Feb 2000 06:43:40 +0000 *F X-Sender: mcbsb@imcb.nus.edu.sg *F X-Mailer: Windows Eudora Light Version 3.0.1 (32) *F Date: Fri, 24 Sep 1999 12:37:12 +0800 *F To: Chihiro Yamada *F From: samibahri *F Subject: Re: EDRC99 - abstract ID60 *F Mime-Version: 1.0 *F Dear Dr Yamada, *F Sorry for the delay in my response. The gene maps to 68F. *F Sami Bahri *F At 10:08 AM 9/23/99 +0100, you wrote: *F >Dear Dr Bahri, *F > *F >Just over a week ago, I sent you a query concerning your abstract for *F >the upcoming EDRC99 conference. I was wondering if you'd had a chance *F >to look at it. I enclose the mail below for your convenience. *F > *F >Thanks *F > *F >Chihiro *F > *F > *F >> From cy200 Tue Sep 14 17:54:51 1999 *F >> To: mcbsb@imcb.nus.edu.sg *F >> Subject: EDRC99 - abstract ID60 *F >> X-Sun-Charset: US-ASCII *F >> Content-Length: 1013 *F >> *F >> Subject: EDRC99 - abstract ID60 *F >> e-mail: mcbsb@imcb.nus.edu.sg *F >> author: Bahri, S. *F >> *F >> Dear Dr. Bahri, *F >> *F >> We are currently curating the abstracts for the upcoming European *F >> Drosophila Research Conference in Zurich, for FlyBase. I am writing in *F >> connection with your abstract: *F >> *F >> 'Characterization of Dsema3a, a Drosophila gene that codes for a new *F >> member of the subclass V semaphorins' *F >> *F >> You mention a gene that is new to FlyBase, Dsema3a. *F >> *F >> Do you have a map location for Dsema3a? It is nice if we can keep as *F >> many gene records as possible anchored to the map. *F >> *F >> Thank you for your help, *F >> *F >> with best wishes, *F >> *F >> Chihiro *F >> ---------------------------------------------------------------------- *F >> Chihiro Yamada. *F >> *F >> FlyBase (Cambridge), *F >> Department of Genetics, *F >> University of Cambridge, *F >> Downing Street, email: c.yamada@gen.cam.ac.uk *F >> Cambridge, CB2 3EH, Ph : 01223-333963 *F >> UK. FAX: 01223-333992 *F >> ---------------------------------------------------------------------- *F >> *F > *F > # *U FBrf0125079 *a Belote *b J. *t 2000.1.25 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: John Belote, Syracuse University *F To: Bloomington Drosophila Stock Center *F Subject: Df(3R)by62 is a simple deficiency *F Dated: 25 Jan 2000 *F *F Background: Df(3R)by62 was originally reported to be a deficient translocation. This pc provides updated cytology for this aberration. Whether the original report was in error or the translocation has healed is unknown. *F If the latter, it is possible that some stocks of Df(3R)by62 retain the translocation. John's cytology was done on a copy of the Bloomington stock. *F *F Information communicated: *F I have examined the polytenes of Df(3R)by62/+ larvae, and I don't see any evidence of a 2:3 translocation. The rearrangment at 85D-F looks like a typical, conventional deficiency with the breakpoints at ~85D10-11 or so, *F and 85F8-11 or so. It seems to me to be deleted for 85F6. I can see 85F12.13 bands but I'm not sure if 85F11 is deleted or not. # *U FBrf0125080 *a Blochlinger *b K. *t 2000.1.29 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Jan 04 20:02:14 2000 *F To: blochli@ix.netcom.com *F Subject: Helping FlyBase: cut-GAL4 *F Dear Karen, *F I think you can help me out here. I am curating a paper for FlyBase *F which uses a cut-GAL4 that we have no previous record of. The paper is *F Guo et al., 1999 *F J. Neurosci. 19(19): 8435--8442 *F and they use a cut-GAL4 for which they thank you. I will make a record *F for this transposon/insertion in FlyBase but would like to get a few *F details so I can do it accurately. Is it a promoter fusion construct *F or is it an enhancer trap insertion of P{GawB}? If it is a promoter *F fusion construct perhaps you could give me an idea of how much of the *F ct regulatory sequences were used in the fusion, or, if it is an *F enhancer trap insertion, please could you tell me whether or not a ct *F phenotype is caused and/or whether you know that the insertion lies *F within the ct transcription unit. Your reply will be curated as a *F personal communication to FlyBase. *F Many thanks for your help. *F With best wishes, *F Rachel. *F From blochli@ix.netcom.com Sun Jan 30 00:47:33 2000 *F From: Karen Blochlinger *F To: Rachel Drysdale (Genetics) *F Subject: ct-GAL4 *F Dear Rachel, *F I've just received the reprint and the description of cut-GAL4 isn't very *F informative. However, I know it can't be an enhancer trap line since we *F didn't do any enhancer trapping. Since it expresses GAL4 along the wing *F margin, I assume it is a GAL4 line we made using the 2.7kb fragment of the *F cut locus that had previously been shown to direct wing margin expression *F (Jack et al. Development 113:735--747, 1991). *F I hope this is helpful. *F Best wishes, *F Karen # *U FBrf0125081 *a Bloomington Drosophila Stock Center *b ?. *t 2000.1.21 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jan 21 21:36:15 2000 *F To: rd120@gen.cam.ac.uk, gm119@gen.cam.ac.uk, cy200@gen.cam.ac.uk *F Subject: CyO, sp* balancer variant *F From: Kevin Cook, Bloomington Drosophila Stock Center *F Subject: CyO, sp* balancer variant *F We received stocks from Ting Wu, Harvard University, balanced over a CyO *F chromosome marked with an unknown allele of speck. The allele is probably *F sp1 rather than a spontaneous sp allele, because the sp-marked CyO was *F recognized in stocks where the nonbalancer homologue was marked with sp1. *F It likely recombined onto CyO, since sp is distal to the most distal CyO *F breakpoint. # *U FBrf0125082 *a Bloomington Drosophila Stock Center *b ?. *t 2000.1.31 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Synonyms for P{ry11} lethal insertion series *F Dated: 31 January 2000 *F *F Background: The P insertions listed below, all of which are associated with lethal or semi-lethal alleles, were represented in the Bloomington stock list for several years with symbols different from those now used by FlyBase. The current symbol and the synonyms previously used at *F Bloomington are listed below. *F *F Information communicated: *F *F Insertion Symbol Previously Used Symbol *F *F 2nd chromosome insertions *F P{ry11}l(2)ry28[1] P{ry11}l(2)28[1] *F P{ry11}l(2)ry36[1] P{ry11}l(2)36[1] *F P{ry11}l(2)ry50[1] P{ry11}l(2)50[1] *F P{ry11}l(2)ry51[1] P{ry11}l(2)51[1] *F P{ry11}l(2)ry53[1] P{ry11}l(2)53[1] *F P{ry11}l(2)ry41[1] P{ry11}l(2)41[1] *F *F 3rd chromosome insertions *F P{ry11}l(3)ry16[1] P{ry11}l(3)16[1] *F P{ry11}l(3)ry59[1] P{ry11}l(3)59[1] *F P{ry11}l(3)ry69[1] P{ry11}l(3)69[1] *F P{ry11}l(3)ry78[1] P{ry11}l(3)78[1] *F P{ry11}l(3)ry82[1] P{ry11}l(3)82[1] *F P{ry11}l(3)ry85[1] P{ry11}l(3)85[1] *F P{ry11}l(3)ry93[1] P{ry11}l(3)93[1] *F P{ry11}l(3)ry103[1] P{ry11}l(3)103[1] *F P{ry11}l(3)ry119[1] P{ry11}l(3)119[1] *F P{ry11}l(3)ry122[1] P{ry11}l(3)122[1] *F P{ry11}l(3)ry133[1] P{ry11}l(3)133[1] *F P{ry11}l(3)ry141[1] P{ry11}l(3)141[1] *F P{ry11}l(3)ry160[1] P{ry11}l(3)160[1] # *U FBrf0125083 *a Bloomington Drosophila Stock Center *b ?. *t 2000.2.12 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: l(3)06713 is not lethal *F Dated: 12 February 2000 *F *F Information communicated: *F *F The BDGP P insertion line called l(3)06713 carries a lethal-free third chromosome. This insertion chromosome is maintained at Bloomington as a healthy stock of homozygotes. The Spradling lab maintains a heterozygous stock of this insertion chromosome over TM3, but *F they must select for heterozygotes to maintain TM3 in the stock (per Nicole Mozden, 2 February 2000), so the insertion chromosome in their stock is also viable rather than lethal. Thus, this insertion is appropriately described as P{PZ}06713 rather than P{PZ}l(3)06713[06713]. # *U FBrf0125084 *a Bloomington Drosophila Stock Center *b ?. *t 2000.2.24 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Df(3R)by62 is a transposition segregant *F Date: 24 February 2000 *F *F Background: There has been confusion over the cytology of Df(3R)by62 due to an error in a table in the initial publication of this aberration (FBrf0069831), which described the aberration as including a translocation. *F *F Information communicated: *F *F The description of Df(3R)by62 as a deficient translocation in FBrf0051508 was an error propagated from a typographical error in FBrf0069831. The original cytological description has been retrieved from the laboratory notebook of Ken Kemphues where the deficiency is clearly described as a transposition segregant. # *U FBrf0125085 *a Bloomington Drosophila Stock Center *b ?. *t 2000.2.7 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Mapping of some P{GawB} insertions *F Date: 7 February 2000 *F *F Background: The chromosomal location of the GAL4 insertions listed below was not known when these lines were added to the collection. We have now mapped these insertions to a chromosome via segregation. A more specific map *F position of the insertion is not known. *F *F Information communicated: *F *F Insertion Maps to chromosome *F P{GawB}V85 1 *F P{GawB}5015 2 *F P{GawB}5108 2 *F P{GawB}5053A 3 # *U FBrf0125086 *a Couso *b J.P. *t 2000.2.10 *T personal communication to FlyBase *u *F From j.p.couso@biols.susx.ac.uk Thu Feb 10 15:43:20 2000 *F To: flybase-help@morgan.harvard.edu *F Subject: ds05142 *F Hi, *F Just to add to Gerard's comments on this allele, we found the same and *F also that ds05142 escapers or pharate adults have a strong ds phenotype; *F in addition, ds05142 does not complement ds1 or ds55. The confusion on *F naming this allele 'al' could be due to the fact that an allele of ds is *F present in the Df(2L)al chromosomes, although this is not often *F reported. *F Juan Pablo Couso. *F \-- *F Please note my new address: *F Dr. Juan Pablo Couso *F School of Biological Sciences *F University of Sussex *F Falmer, Brighton BN1 9QG *F Phone: 44- (0)1273 877448 (direct line) *F fax: 44- (0)1273 678433 *F e-mail: J.P.Couso@biols.susx.ac.uk # *U FBrf0125087 *a Davis *b R. *t 2000.2.1 *T personal communication to FlyBase *u *F From richardd@bcm.tmc.edu Tue Feb 01 19:50:36 2000 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 1 Feb 2000 19:50:36 +0000 *F Mime-Version: 1.0 *F Date: Tue, 1 Feb 2000 14:01:42 -0600 *F To: rd120@gen.cam.ac.uk *F From: 'Richard J. Davis' *F Subject: Flybase Curating *F Rachel- *F >I have the information for curating the *F following paper for FlyBase: *F Genes Dev. 13(24): 3231--3243 *F Heanue et al 1999 *F Synergistic regulation of ...... *F 1) The 'dac-Gal4' driver is an enhancer trap is a phenotypic null allele of *F dac. *F 2) The identifier for the chromosome used in the paper is: dac-GAL4p7d#23, H1-1 *F . *F . *F The P{GAL4}dacp7d23 is a GawB element. Please attribute the allele to *F Graeme Mardon. *F Richard J. Davis, Ph.D. *F Department of Pathology, S242 *F Baylor College of Medicine *F Houston, TX 77030 *F Tel: (713) 798-8715 *F Fax: (713) 798-8920 *F email: richardd@bcm.tmc.edu # *U FBrf0125088 *a Eeken *b J. *t 2000.2.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Feb 29 19:38:11 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Region 23CD mutations *F From: Kevin Cook, Bloomington Stock Center *F Subject: Region 23CD mutations *F The following information accompanied fly stocks donated to the Stock *F Center (2/00) by Jan Eeken, Leiden University. *F An EMS screen for lethal mutations that fail to complement Df(2L)JS17 *F identified 14 complementation groups. The mutations were induced on a *F cn1 bw1 chromosome. *F Compl. group Allele Df mapping *F l(2)23Ca A16-1 Not included within Df(2L)JS7 or C28 *F l(2)23Cb A18-6 Not included within Df(2L)JS7 or C28 *F l(2)23Cc A17-2 Not included within Df(2L)JS7 or C28 *F l(2)23Cd A7-4 Not included within Df(2L)JS7 or C28 *F l(2)23Ce A6-2 Not included within Df(2L)JS7 or C28 *F l(2)23CDa A1-6 Included within Df(2L)JS7, but not Df(2L)C28 *F l(2)23CDb A1-5 Included within Df(2L)JS7, but not Df(2L)C28 *F l(2)23CDc A1-4 Included within Df(2L)JS7, but not Df(2L)C28 *F toc A1-1 Included within Df(2L)JS7, but not Df(2L)C28 *F l(2)23CDd A9-3 Included within Df(2L)JS7, but not Df(2L)C28 *F l(2)23CDe A8-1 Included within Df(2L)JS7, but not Df(2L)C28 *F l(2)23CDf A13-1 Included within Df(2L)JS7, but not Df(2L)C28 *F l(2)23Da A5-2 Included within Df(2L)C28, but not Df(2L)JS7 *F l(2)23Db A18-1 Included within Df(2L)C28, but not Df(2L)JS7 *F The relative positions of complementation groups within the three intervals *F is not known. *F l(2)23CaA16-1 is semilethal. *F This screen was described in Eeken, J.C., Kooistra, R., Lohman, P.H., *F Pastink, A. and Szakmary, A. 1997, Isolation and characterization of two *F MMS-sensitive mutants in region 23C-23D. A. Dros. Res. Conf. 38: 272A *F FBrf0092383. # *U FBrf0125090 *a Gatt *b M. *c M. *d Ashburner *t 2000.2.3 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Thu Feb 03 17:42:24 2000 *F To: ag24@gen.cam.ac.uk *F Subject: gene merges in X tip with a pc *F Personal communication from M. Gatt & M. Ashburner to FlyBase. *F 1. Please merge EG:115C2.8 and anon-1BCb, as EG:115C2.8. *F Position, direction, both wrt su(s) and transcript size *F are consistent with this being EG:115C2.8. *F 2. Please merge ESTS:99F6T and EG:BACR7A4.20 as EG:BACR7A4.20. *F Based on sequence identity. *F 3. Please merge A3-3 & EG:33C11 as A3-3. *F - size of protein, both 613aa *F - both have bZIP domains *F A3-3 said to be match to Human ATF3; BLASTP of this (P18847) against *F fly has EG:33C11.1 as its best match - though not impressive *F score = 121; expect 1.6e-11. BLASTP with Human FOS (P01100) hits *F EG:33C11.1 as second best. *F 4. Please merge anon-2Bd & EG:63B12.2 as EG:63B12.2. *F This is on the evidence that both are in the very short region between *F trr and arm; direction and size are consistent. *F 5. Please merge anon-2Ea & Cyp4e4. *F Identity by virtue of size, direction, position & restriction *F enzyme maps. *F 6. Please merge anon-2Eb & EG:152A3.1 as EG:152A3.1. *F Identity by virtue of size, direction, position & restriction *F enzyme maps. *F 7. anon-3Bd & EG:95B7.9 as EG:95B7.9. *F Identity by virtue of position, direction, size & restriction map. *F 8. anon-3Be & EG:95B7.7 as EG:95B7.7. *F Identity by virtue of position & restriction map. *F 9. dwg & EG:95B7.6 as dwg. *F Identity by sequence identity. *F 10. ptr & EG:BACH48C10.5 as ptr. *F Identity by virtue of position, direction, size and restriction map. *F 11. anon-1Eb & EG:BACR7A4.9 as EG:BACR7A4.9. *F anon-1Ec & CDC45L *F anon-1Ed & EG:BACR7A4.8 as EG:BACR7A4.8. *F All by virtue of restriction maps, direction and position. # *U FBrf0125091 *a Gatt *b M. *c M. *d Ashburner *t 2000.2.8 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Tue Feb 08 16:41:15 2000 *F To: rd120@gen.cam.ac.uk, ma11@gen.cam.ac.uk *F Subject: PC to FlyBase *F Personal communication to FlyBase from M. Gatt & M. Ashburner. *F anon-3Ba is EG:100G10.7 on basis of size & restriction map. *F anon-3Bb is EG:100G10.5 on basis of size, restriction map & direction. *F Note that the direction of transcription of anon-3Ba is opposite to that *F of EG:100G10.7. However, everything else fits (indeed the match between *F the restriction maps is excellent, and, the direction of transcription *F of anon-3B1.2 (as 0.9kb transcript) shown in the relevant figure, *F figure 2 of Reddy et al. (1984) Cell 38:701-710, is wrong, since *F even the database accession says that it is off the opposite strand to per *F and not the same (as in fig 2 here). So we have little concern that the *F authors got the direction of transcription of the 2.7-kb RNA (aka anon-3Ba) *F wrong. *F In conclusion the revised gene order statement should be: *F per+ anon-3B1.2- EG:100G10.7- EG:100G10.5- # *U FBrf0125092 *a Gatt *b M. *c M. *d Ashburner *t 2000.2.9 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Tue Feb 08 17:00:08 2000 *F To: rd120@gen.cam.ac.uk, ma11@gen.cam.ac.uk *F Subject: pc from ma and mg *F Personal communication to FlyBase from M. Gatt and M. Ashburner. *F anon-2Db is EG:152A3.7 by criteria of position & restriction map. *F Size of the EG:152A3.7 is a bit smaller than that reported for anon-2Db, *F but the EH sequence could well lack 5' &/or 3' utr's. # *U FBrf0125093 *a Gatt *b M. *t 2000.1.20 *T personal communication to FlyBase *u *F From mkg23@mole.bio.cam.ac.uk Thu Jan 20 14:50:21 2000 *F To: 'Michael Ashburner (Genetics)' *F Subject: From this morning... *F A comparison of *F a). the positions of predicted genes in the EDGP sequence and *F b). restriction maps from the EDGP sequence *F with the data provided by Juan (see below) indicates that *F l(1)EC4 = EG:165H7.3. *F FROM JUAN: *F Francisco González, in his Ph.D. thesis (González, 1989), *F started to characterize the region proximal from AS-C T1. He *F obtained a lambda phage (lsc7R) extending for ca. 12 kb *F proximally from the end of our walk in the AS-C (which is 9.5 *F kb proximally from the start of ase) and found that it *F hybridized to several transcripts (1.0, 0.8 and 5.5 kb). The *F 5.5 kb transcript was present in embryos, larva, early pupa *F and adults. It was lit up by each of the four fragments (Eco- *F Bam4.1 kb (fragment I), Bam-Bam4.2 kb (fragment II), Bam- *F Bam3.2 kb (fragment III), and Bam-Eco1.2 kb (fragment IV), *F distally to proximally) that comprised the 12 kb insert of *F lsc7R, suggesting a transcription unit that gives rise to a *F transcript which is subsequently spliced. He also found in *F southern blots the following modifications: Df(1)260.1 *F modifies frag. I. So prox. breakpoint of Df. at 9.5 to 13.5 *F kb to the left of start of ase. Df(1)scB57 modifies frag. II. *F So prox. breakpoint of Df. at 13.5 to 17.7 kb to the left of *F start of ase. l(1)EC4S1 modifies frag. I and l(1)EC4S211 *F modifies frag. II. So, we think that the transcript *F corresponds to the gene l(1)EC4 found by Kalpana White, as *F cited by Jimenez and Campos-Ortega (Jiménez and Campos- *F Ortega, 1987). Whether EG:165H7.3 or any of the more proximal *F predicted genes up to svr correspond to l(1)EC4, I do not *F know. You could probably find out by comparing the positions *F of the predicted genes with the above data....... *F The genetic analyses of Kalpana also placed *F l(1)EC5 between l(1)EC4 and svr. *F With regards to the data on l(1)EC4, the BamH1 site in *F between the 4.0 Eco-Bam and the 4.2 Bam-Bam fragments (the *F two fragments modified in the l(1)EC4S1 and l(1)EC4S211 *F alleles, respectively, which also contain the breakpoints for *F Df(1)260.1 and Df(1)scB57, respectively again) is at position *F 30.771 of the cosmid 165H7 sequence, acces. n. AL009188. *F Encarna dixit. It may help you in identifying one of the *F predicted genes with l(1)EC4 # *U FBrf0125094 *a Gatt *b M. *t 2000.1.20 *T personal communication to FlyBase *u *F From mkg23@mole.bio.cam.ac.uk Thu Jan 20 17:16:07 2000 *F To: 'Michael Ashburner (Genetics)' *F Subject: Re: From this morning... *F Sequence comparison between EDGP sequence and the published sequence *F from Begley D, Murphy AM, Hiu C, Tsubota SI (1995). Modifier of rudimentary *F p1, mod(r)p1, a trans-acting regulatory mutation of rudimentary. Mol *F Gen Genet Jul 22;248(1):69-78 indicates that EG:115C2.5 = mod(r) # *U FBrf0125095 *a Hirsh *b J. *t 1991.11.1 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Jay Hirsh, University of Virginia *F To: Bloomington Drosophila Stock Center *F Subject: map location of P{ry11}l(4)ry16[1] *F Date: 1 November 1991 *F *F Information communicated: *F *F The insertion site of P{ry11}l(4)ry16[1] is 102D, determined by in situ hybridization. # *U FBrf0125096 *a Lewis *b J. *t 2000.2.3 *T personal communication to FlyBase *u *F From jlewis@holyrood.ed.ac.uk Thu Feb 03 11:28:31 2000 *F To: Michael Ashburner (Genetics) *F Subject: Re: Question about Cbp20 *F Dear Michael, *F the cosmid that you are describing contains part of CBP80 *F which is accession number AJ238970 not AJ238969. I checked the CBP80 entry *F (AJ238970) and you are correct that the map data is wrong, it maps to *F 4C7-8. I'll notify the correction to EMBL. CBP20 maps to 90E1-2. *F . *F . *F Best wishes *F Joe *F Dr. Joe D. Lewis *F University of Edinburgh *F Institute of Cell and Molecular Biology *F Michael Swann Building *F The King's Buildings *F Mayfield Road *F Edinburgh EH9 3JR *F UNITED KINGDOM *F tel: (44) 131 650 7117/7103 *F Fax: (44) 131 650 7116 *F e-mail: joe.lewis@ed.ac.uk # *U FBrf0125097 *a Luo *b L. *t 2000.1.20 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Jan 20 11:25:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 20 Jan 2000 11:25:56 +0000 *F To: lluo@stanford.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 20 Jan 2000 11:29:21 +0000 *F Content-Length: 1485 *F Dear Dr. Luo, *F I am curating your DIS article for FlyBase: *F Luo et al., 1999, D. I. S. 82: 102--105 *F In this you describe an enhancer trap vector PFLP. I am writing to *F ask for some more details about this construct, such as the marker *F allele in the vector and the promoter that drives the FLP gene. This *F information would be recorded in FlyBase as a 'personal communication to FlyBase *F from you to FlyBase. *F You state that you 'inserted the open reading frame of the yeast FLP *F recombinase into an enhancer trap vector' which comes from: *F Giniger et al., 1993, Roux Arch. dev. Biol. 202: 112--122 *F The enhancer trap vector in that paper is 'P{KZ.TRAP}', which contains *F a kinesin-lacZ fusion gene expressed under the control of a P-element *F transposase promoter. *F Did you just replace the kinesin-lacZ fusion gene with FLP sequences to *F make your PFLP vector ? - then I will know that the promoter driving *F the FLP coding region is the P-element transposase promoter, and also *F that the marker gene is w, since this is the marker gene in the *F P{KZ.TRAP} vector, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From lluo@leland.stanford.edu Thu Jan 20 15:11:48 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 20 Jan 2000 15:11:48 +0000 *F Mime-Version: 1.0 *F X-Sender: lluo@popserver4.stanford.edu *F Date: Thu, 20 Jan 2000 07:14:43 -0800 *F To: Gillian Millburn (Genetics) *F From: Liqun Luo *F Subject: Re: FlyBase query *F Dear Dr. Millburn: *F >Did you just replace the kinesin-lacZ fusion gene with FLP sequences to *F >make your PFLP vector ? *F Yes we did insert FLP to replace KZ without changing anything else to *F the vector. *F >- then I will know that the promoter driving *F >the FLP coding region is the P-element transposase promoter, and also *F >that the marker gene is w, since this is the marker gene in the *F >P{KZ.TRAP} vector, *F That's correct. *F Best wishes, *F Liqun Luo *F =================================================================== *F Liqun Luo, Ph. D. Tel: (650)723-6645 *F Assistant Professor Fax: (650)723-0589 *F Department of Biological Sciences Dept Fax: (650)723-6132 *F Herrin Labs 144A E-mail: lluo@stanford.edu *F 385 Serra Mall *F Stanford University *F Stanford, CA 94305-5020 *F =================================================================== # *U FBrf0125098 *a Mlodzik *b M. *t 2000.1.26 *T personal communication to FlyBase *u *F From Marek.Mlodzik@embl-heidelberg.de Wed Jan 26 11:56:33 2000 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Wed, 26 Jan 2000 11:56:33 +0000 *F Date: Wed, 26 Jan 2000 12:59:59 +0100 (MET) *F Mime-Version: 1.0 *F To: Chihiro Yamada *F From: Marek Mlodzik *F Subject: Re: FlyBase Query (cy298) *F We have send them a sca-GAL4 line called T3 (it doesn't have a phenotype by *F itself). The 73-1 and 8-1 lines described in Lee et al. are *F loss-of-function sca alleles. *F Hope this is helpful, *F Regards, *F Marek # *U FBrf0125099 *a Portin *b P. *t 2000.2.3 *T personal communication to FlyBase *u Notch affects the development of Drosophila macrochaeta through lateral inhibition but that of wing veins through induction. *F Archived. # *U FBrf0125100 *a Posakony *b J. *t 2000.2.8 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Feb 02 14:28:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 2 Feb 2000 14:28:50 +0000 *F To: jposakony@ucsd.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 2 Feb 2000 14:32:23 +0000 *F Content-Length: 1868 *F Dear Dr. Posakony, *F I am curating your paper for FlyBase: *F Lai et al., 2000, Development 127(2): 291--306 *F and I have a question. *F . *F . *F 1. UAS-Bob *F I need to make a record for this construct in FlyBase and an allele of *F Bob to record the phenotypes caused by overexpression of Bob. *F However, I need to know which Bob gene (A,B, C or D) to put the allele *F under. In the materials and methods you state that you used 'PCR to *F amplify the coding regions and 8-10nt of 5' UTR sequence' of Bob. *F Since in the results it says that there are 'small differences in the *F transcribed regions of the different Bob genomic loci' does this mean *F that you can say which copy of Bob the coding region in UAS-Bob *F corresponds to, or can you say that for example it could be A,B, or C *F but definately isn't D. *F If you do not know which Bob coding region it corresponds to, I will *F probably put the allele for UAS-Bob under BobA, and include a note that *F it could actually correspond to BobB, BobC or BobD (?) since the coding *F regions are identical (at least for A-C). *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From jposakony@ucsd.edu Tue Feb 08 20:42:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 8 Feb 2000 20:42:15 +0000 *F Mime-Version: 1.0 *F X-Sender: posakony@biomail.ucsd.edu *F Date: Tue, 8 Feb 2000 12:49:17 -0800 *F To: Gillian Millburn (Genetics) *F From: Jim Posakony *F Subject: Re: FlyBase query *F >Dear Dr. Posakony, *F > *F >I am curating your paper for FlyBase: *F > *F >Lai et al., 2000, Development 127(2): 291--306 *F > *F >and I have a question. *F . *F . *F > *F > *F >1. UAS-Bob *F > *F >I need to make a record for this construct in FlyBase and an allele of *F >Bob to record the phenotypes caused by overexpression of Bob. *F > *F >However, I need to know which Bob gene (A,B, C or D) to put the allele *F >under. In the materials and methods you state that you used 'PCR to *F >amplify the coding regions and 8-10nt of 5' UTR sequence' of Bob. *F > *F >Since in the results it says that there are 'small differences in the *F >transcribed regions of the different Bob genomic loci' does this mean *F >that you can say which copy of Bob the coding region in UAS-Bob *F >corresponds to, or can you say that for example it could be A,B, or C *F >but definately isn't D. *F The UAS-Bob construct we used was made from a cDNA clone we recovered *F called Bob2. In the coding region (used to make the UAS construct), *F this clone matches our sequence of Bob A perfectly, and has a *F one-base conservative substitution difference from our sequences of *F Bob B and Bob C (i.e., the predicted protein product of the UAS *F transgene is identical to that of Bob A, B, or C). It definitely does *F not correspond to 'Bob D', which as we said in the paper just *F reflects the sequence of the CK EST. *F Best wishes, *F Jim Posakony # *U FBrf0125101 *a Preiss *b A. *t 2000.2.18 *T personal communication to FlyBase *u *F From preiss@uni-hohenheim.de Fri Feb 18 08:25:10 2000 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Fri, 18 Feb 2000 08:25:10 +0000 *F Date: Fri, 18 Feb 2000 09:29:02 +0100 (MEZ) *F From: Anette Preiss *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (cy360) *F Dear Chihiro Yamada, *F hopefully the answers enclosed will be helpful. *F 1. gro alleles. *F These 5 alleles are described in *F Preiss, A., D. Hartley and S. Artavanis-Tsakonas (1988). *F The molecular genetics of Enhancer of split, a gene required for embryonic *F neural development in Drosophila. EMBO J. 7: 3917-3928 *F In Lindsley and Zimm (1992), they were renamed r17 - r22, however, these *F names are not commonly used in the literature. *F All five are EMS induced alleles in an e4 tx background. They uncover the *F recessive phenotype of gro1 and are lethal in trans and over deficiencies. *F Therefore, they were named alleles of l(3) gro. *F The mutations all affect the transcription unit m9/m10 in the E(spl) *F region because they can be rescued with a respective trans-gene. *F I am not aware that any of these alleles affect the neighboring *F transcription unit E(spl) bHLH m8 and it seems rather unlikely because *F they do not show any obvious molecular or cytological aberrations. *F However, as long as there are no sequence data of these alleles available, *F a double hit in m8 cannot be excluded. As far as I can tell, mutations in *F m8 do not give any apparent phenotype. Therefore, a separation of *F mutations in the two genes can only occur at the molecular level. *F There is some confusion in naming the genes in the E(spl) region which is *F based on the problems in their original characterization. Two 'historical' *F alleles, gro and E(spl) have been name giving. E(spl) is a mutation in m8 *F which has little apparent phenotype on its own but enhances the eye *F roughening of the recessive split allele of Notch in a dominant manner *F (thus: E(spl)D). Revertants of E(spl)D were generated that are mostly *F structural mutations (deletions and translocations) which affect several *F genes in the region. Nearly all of them affect the neighboring gro gene *F with exception of the three mutations mentioned in the paper. This is why *F the name E(spl) was extended towards the whole region and the respective *F genes that are further identified by their transcription unit label (m8 - *F mdelta). *F .. *F Best regards, Anette Preiss *F \---------------------------------------------------------------------- *F Prof. Dr. Anette Preiss *F University of Hohenheim *F Department of Genetics (240) Ph: ++49-711-459 2206 *F 70593 Stuttgart FAX: ++49-711-459 2211 *F F.R. Germany e-mail: preiss@uni-hohenheim.de *F \---------------------------------------------------------------------- *F From cy200 Wed Feb 16 12:04:56 2000 *F To: preiss@uni-hohenheim.de *F Subject: FlyBase Query (cy360) *F Cc: me *F X-Sun-Charset: US-ASCII *F Content-Length: 1610 *F Dear Dr Preiss, *F I am currently curating your paper for FlyBase: *F Nagel et al., 1999, Dev. Genet. 25(2): 168--179 *F I have a few questions I was hoping you could help me with. *F 1. gro alleles. *F In your materials and methods section, you mention a number of gro *F alleles: E28, E48, E73, E75, E105. Looking through our records, we *F don't have all of these as alleles of gro, some of these appear as *F alleles of m8. Are these all definitely alleles of gro? Are they *F also alleles of m8? If they are alleles of both, are the gro and m8 *F phenotypes known to be separable? It is not clear from our records *F what the situation is for these alleles and it would be very helpful if *F you could help me sort this out. *F .. *F Any information you give would be entered as a 'personal communication to FlyBase *F from you to FlyBase, provided you are happy with that. *F Thanks and Best wishes, *F Chihiro *F \------------------------------------ *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0125102 *a Rebay *b I. *t 2000.2.25 *T personal communication to FlyBase *u *F From rebay@wi.mit.edu Fri Feb 25 13:42:59 2000 *F Subject: RE: Helping FlyBase: Df(2L)PMF47c *F To: 'Genetics' *F Rachel, *F The inversion allele is called spenXLS1000 and its breakpoints according to our cytology are In(2LR)21B;55E *F Ilaria # *U FBrf0125103 *a Schaefer *b U. *c H. *d Jackle *e Y. *f He *g H. *h Bellen *i T. *j Laverty *k G. *l Rubin *t 1999.12.2 *T personal communication to FlyBase *u *F Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Subject: Goettingen lethals - set 4 *F Dated: 1 December 1999 *F *F Background: The lethal and semi-lethal alleles and P{lacW} insertions reported here were generated in the laboratory of *F Herbert Jackle (Goettingen) in a screen for lethal P{lacW} hops into the X chromosome. In situ hybridizations to map the *F P{lacW} insertions were carried out in the laboratory of Hugo Bellen (Houston). Hybridized chromosomes were read by *F both the Bellen lab and by Todd Laverty (Rubin lab, Berkeley). The assumption that lethality in single insertion lines is *F caused by the insertion has not been verified. lacZ expression patterns for these lines will be available from FlyView. *F This communication includes revised insertion sites for four of the lines included in FBrf0108529, followed by new alleles *F and transposon insertions. *F *F Alleles with updated information (presumed to be caused by P{lacW} insertions): *F Original Update *F l(1)G0003G0003 17C 17D *F l(1)G0103G0103 12E1-4 12E *F l(1)G0228G0228 7E3-6 7E5-6 *F l(1)G0229G0229 13C1-2 13B6-9 *F *F New Alleles (presumed to be caused by P{lacW} insertions): *F l(1)G0257G0257 ? -- multi-insert line: 1B, 2C *F l(1)G0319G0319 1B7-10 *F l(1)G0471G0471 1B11-14 *F l(1)G0109G0109 1C *F l(1)G0422G0422 1C *F l(1)G0451G0451 1C *F l(1)G0192G0192 2B *F l(1)G0450G0450 2B *F l(1)G0500G0500 2C1-2 *F l(1)G0184G0184 ? -- multi-insert line: 2D1-2, 9E1-4 *F l(1)G0226G0226 ? -- multi-insert line: 2F, 4C7-8 *F l(1)G0475G0475 3A *F l(1)G0488G0488 ? -- multi-insert line: 3A, 7F *F l(1)G0362G0362 3A3-4 *F l(1)G0359G0359 3D1-4 *F l(1)G0279G0279 3E3-7 *F l(1)G0501G0501 3E5-F2 *F l(1)G0231G0231 3F -- semi-lethal *F l(1)G0048G0048 4AB *F l(1)G0032G0032 4B *F l(1)G0204G0204 4C7-8 *F l(1)G0326G0326 4C7-8 *F l(1)G0010G0010 5C5-8 *F l(1)G0486G0486 5C5-10 *F l(1)G0489G0489 5E *F l(1)G0114G0114 5F *F l(1)G0461G0461 6B-C *F l(1)G0087G0087 6E -- semi-lethal *F l(1)G0306G0306 7C -- semi-lethal *F mysG0091b 7D3-7 (from multi-insert line) *F l(1)G0348G0348 7D -- semi-lethal *F l(1)G0459G0459 7D -- semi-lethal *F l(1)G0093G0093 7D3-4 *F l(1)G0477G0477 7D14-E2 *F l(1)G0295G0295 7E -- semi-lethal *F l(1)G0425G0425 7E *F l(1)G0376G0376 7E5-10 *F l(1)G0413G0413 7F *F l(1)G0490G0490 8C4-10 *F l(1)G0464G0464 8F *F l(1)G0470G0470 8F4-7 -- semi-lethal *F l(1)G0197G0197 9B *F l(1)G0002G0002 9E1-4 *F l(1)G0386G0386 9F *F l(1)G0478G0478 ? -- multi-insert line: 10C1-2, 12C-D *F l(1)G0341G0341 10E *F l(1)G0214G0214 11A -- semi-lethal *F l(1)G0366G0366 11B5-12 *F l(1)G0208G0208 11D -- semi-lethal *F l(1)G0443G0443 11E-F *F l(1)G0122G0122 12A-B *F l(1)G0031G0031 12C *F l(1)G0495G0495 12C *F l(1)G0472G0472 12C-D -- semi-lethal *F l(1)G0487G0487 12C4-8 *F l(1)G0467G0467 12D *F l(1)G0308G0308 12E *F l(1)G0491G0491 12E *F l(1)G0176G0176 12E3-7 *F l(1)G0469G0469 12E3-7 *F l(1)G0185G0185 12E5-7 *F l(1)G0498G0498 12F3-7 *F l(1)G0344G0344 13A1-6 -- semi-lethal *F l(1)G0134G0134 13B08-9 *F l(1)G0442G0442 13E08-9 *F l(1)G0483G0483 13F1-2 *F l(1)G0347G0347 14B1-4 -- semi-lethal *F l(1)G0484G0484 15F *F l(1)G0297G0297 17C-D *F l(1)G0493G0493 18D *F l(1)G0447G0447 18D1-4 -- semi-lethal *F l(1)G0225G0225 18F -- semi-lethal *F l(1)G0337G0337 19E *F *F New Transposon insertions (from multi-insert lines): *F P{lacW}G0091a 3E1-2 *F P{lacW}G0257a 1B *F P{lacW}G0257b 2C *F P{lacW}G0184a 2D1-2 *F P{lacW}G0184b 9E1-4 *F P{lacW}G0226a 2F *F P{lacW}G0226b 4C7-8 *F P{lacW}G0478a 10C1-2 *F P{lacW}G0478b 12C-D *F P{lacW}G0488a 3A *F P{lacW}G0488b 7F # *U FBrf0125105 *a Sousa-Neves *b R. *t 2000.1.20 *T personal communication to FlyBase *u *F From asneves@visualnet.com.br Thu Jan 20 23:40:11 2000 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: M(1)18C *F Dear Rachel, *F Could you please add to Flybase report that T(1;4)sc H is duplicated for *F yellow ( that is something I forgot to tell you the last time because it *F seemed obvious). *F Could you also state that Dp(4;3)f fails to rescue pho? *F Best wishes *F Rui *F Rui Sousa-Neves *F Rua Almirante Alexandrino, 2609- apto 201 *F CEP 20241-261, Santa Teresa, *F Rio de Janeiro, RJ, Brazil # *U FBrf0125106 *a Talbert *b P.B. *c S. *d Henikoff *t 2000.1.29 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Jan 28 13:27:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Jan 2000 13:27:18 +0000 *F To: steveh@fhcrc.org *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 28 Jan 2000 13:30:49 +0000 *F Content-Length: 1553 *F Dear Dr. Henikoff, *F I am curating your paper for FlyBase: *F Talbert and Henikoff, 2000, Genetics 154(1): 259--272 *F and I have a question about the 'McT' stock. *F This is the stock that is In(1)wmMc,wmMc *F ( = superscript) *F On page 267, you say that the 'McT chromosome is actually an X.YL *F translocation with the point of exchange in the rDNA'. *F At the moment FlyBase has that In(1)wmMc is an inversion with *F breakpoints at 3C1-3C2 and 20F. *F What I need to know is whether you think *F 1. that the X.YL translocation that is in your McT stock has occurred *F 'on top of' the original In(1)wmMc inversion *F or *F 2. whether the original In(1)wmMc inversion was really a X.YL *F translocation that had not been noticed before. *F It makes a difference in FlyBase as to which is the case: *F if you think 1. is true then I will make a new aberration in FlyBase, *F called I guess T(1;Y)McT, with In(1)wmMc as the progenitor chromosome *F if you think 2. is true then I will not make a new chromosome, but will *F record that In(1)wmMc is actually a T(1;Y) (as well as the inversion) *F and will correct the breakpoints in FlyBase. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From steveh@muller.fhcrc.org Fri Jan 28 16:49:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Jan 2000 16:49:58 +0000 *F Date: Fri, 28 Jan 2000 08:53:00 -0800 *F From: steveh@muller.fhcrc.org (Steve Henikoff) *F To: gm119@gen.cam.ac.uk *F Subject: Re: FlyBase query *F Cc: ptalbert@fred.fhcrc.org *F Content-Length: 1106 *F Hi Gillian, *F Our evidence favors 1. You'll notice that the wmMc attributable to Oster *F (a student of Muller's) is just as described originally by Muller both *F in being an In(1) and showing a variegating phenotype stronger than wm4. *F The T(1;Y) that has been used in many labs and is the subject of other *F papers that we refer to is just what we would have expected if during *F stock maintenance an X;Y translocation had occurred on top of the In(1), *F and we speculated that maintenance alone would favor such events: *F X;Y translocations that occur between the X and Y rDNA happen frequently *F (that's how you detach attached XXs) and because there may be a selective *F advantage in suppressing the rst and/or other variegating phenotypes by *F added heterochromatin (the bulk of the Y chromosome). Paul Talbert did this *F work, and he can correct me if I'm in error on this. With respect to what *F to call it, I suppose that 'McT' is appropriate, because that would work *F whether or not you name it after Ken Tartof who first described the *F phenotype or Paul Talbert who described the genotype. *F Best regards, *F \--Steve Henikoff *F From paul@muller.fhcrc.org Fri Jan 28 20:11:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Jan 2000 20:11:49 +0000 *F Date: Fri, 28 Jan 2000 12:13:58 -0800 (PST) *F From: Paul Talbert *F X-Sender: paul@sparky.fhcrc.org *F Reply-To: Paul Talbert *F To: Steve Henikoff *F cc: gm119@gen.cam.ac.uk, ptalbert@fred.fhcrc.org *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Gillian and Steve: *F I missed the original question here, but let me try to make sure we're all *F in agreement about which stock is which. The stock that behaves like *F Muller's original description of In(1)w[mMc] is the In(1)w[m4L]w[mMcR],v / *F Df(YS)bb stock, which I called '4LMcR' in the paper. I received this stock *F as Mid-America stock #1130. Probably this stock was generated by Muller *F and is the basis of his claim that the left ends of In(1)w[m4] and *F In(1)w[mMc] can be exchanged without affecting their phenotypes. Muller *F also reported that both reciprocal recombinants were bb+, and this is *F consistent with the stock being kept over Df(YS)bb. The presumed *F reciprocal recombinant, originally labeled In(1)w[mMcL]w[m4R], f / *F Df(YS)bb (Mid-America stock #1121 or my '4f') I found to have both ends of *F w[m4]; presumably Muller isolated a double recombinant moving f onto *F In(1)w[m4]. *F The original (non-recombinant, non-translocated) In(1)w[mMc] seems to have *F been lost, although Art Hilliker and Tom Grigliatti both seem to have had *F it in the 80s. Art told me his stock definitely did not have the T(1;Y). I *F don't know whether Tom looked at the mitotic chromosomes of his stock, but *F the stock used by Sinclair et al. (1989) behaves genetically like what I *F expect for the original In(1)w[mMc]. Vett LLoyd told me that the *F Grigliatti lab did not maintain this stock; the stock later used in the *F Lloyd et al. 1997 paper was a different stock and behaves like the T(1;Y) *F chromosome. Vett said they got the latter stock from another lab (she *F wasn't sure which one). Probably her stock and Tartof's stock ('McT') both *F derive from the stock that is now Bloomington stock #4478 ('McB'). This *F stock was formerly a Mid-America stock from the Oster collection, i.e. it *F was the original In(1)w[mMc] stock which must have acquired the T(1;Y) *F prior to Tartof's report of it in 1984, probably around 1980 or so since *F the untranslocated stock still existed in other labs during the 80s. *F With respect to what *F > to call it, I suppose that 'McT' is appropriate, because that would work *F > whether or not you name it after Ken Tartof who first described the *F > phenotype or Paul Talbert who described the genotype. *F Shouldn't the proper name be something like 'X.YL, In(1)w[mMc], w[mMc]' *F or 'C(1;Y)1, In(1)w[mMc], w[mMc]'? *F Currently on Flybase C(1;Y)1 seems to cover this entire class of *F translocations. Presumably any of them would have the same effect on *F suppressing variegation, so naming this particular translocation based on *F its effect on w[mMc] (such as 'T(1;Y)McT, In(1)w[mMc], w[mMc]') seems *F superfluous to me. *F Paul Talbert *F Fred Hutchinson Cancer Research Center *F 1100 Fairview Avenue N, A1-162 *F Seattle, WA 98109 *F Phone: 206-667-4509 *F Fax: 206-667-5889 *F paul@muller.fhcrc.org *F From paul@muller.fhcrc.org Sat Jan 29 00:36:41 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 29 Jan 2000 00:36:41 +0000 *F Date: Fri, 28 Jan 2000 16:38:52 -0800 (PST) *F From: Paul Talbert *F X-Sender: paul@sparky.fhcrc.org *F Reply-To: Paul Talbert *F To: Steve Henikoff *F cc: gm119@gen.cam.ac.uk, ptalbert@fred.fhcrc.org *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Gillian: *F I think it should be clear from my previous message, but for *F purposes of annotating Flybase, I believe the following information is *F most likely to be true: *F Rearrangement: In(1)w[mMc] *F References: Muller 1946, DIS 20: 66-68 *F Hilliker and Appels 1982, Chromosoma 86: 469-490. *F Sinclair et al. 1989, Mol. Gen. Genet. 216: 328-333. *F Stocks: None known. *F Rearrangement: In(1)w[m4L]w[mMcR] *F References: Muller 1946, DIS 20: 66-68 *F Talbert and Henikoff 2000, Genetics 154:259-272. *F Stocks: Henikoff lab *F Rearrangement: In(1)w[mMcL]w[m4R] *F References: Muller 1946, DIS 20: 66-68 *F Stocks: None known *F Rearrangement: C(1;Y)1, In(1)w[mMc] *F References: Tartof et al. 1984, Cell 37: 869-878. *F Locke et al. 1988, Genetics 120: 181-198. *F Tartof et al. 1989, Dev. Genet. 10: 162-176. *F Martin-Morris et al. 1997, Genetics 147: 671-677 *F Lloyd et al. 1997, Genetics 145:945-959. *F Talbert and Henikoff 2000, Genetics 154:259-272. *F Stocks: Bloomington #4478 *F Rearrangements: C(1;Y)1, In(1)w[mMcL]w[m4R] and *F C(1;Y)1, In(1)w[m4L]w[mMcR] *F References: Lloyd et al. 1997, Genetics 145:945-959. *F Stocks: None? *F Paul Talbert *F Fred Hutchinson Cancer Research Center *F 1100 Fairview Avenue N, A1-162 *F Seattle, WA 98109 *F Phone: 206-667-4509 *F Fax: 206-667-5889 *F paul@muller.fhcrc.org # *U FBrf0125107 *a Wu *b T. *t 2000.1.28 *T personal communication to FlyBase *u *F From tobywu@u.washington.edu Fri Jan 28 05:47:01 2000 *F To: flybase-help@morgan.harvard.edu *F Subject: molecular data *F Dear FlyBase, *F My name is Toby Wu and I am an undergraduate working in Dr. Celeste Berg's *F laboratory at the University of Washington. Two years ago, I sequenced two *F alleles of Drosophila ras, Ras85DSutor-341 and Ras85DSutor-404, as part of *F my project. I have included the molecular data for these two alleles *F below. *F Ras85DSutor-341 consists of a nonsense mutation that affects amino acid *F \#131 (Arg to Stop). The insertion of a new stop codon 3bp upstream of the *F second intron results in truncation of the protein, thereby eliminating *F the CAAX box. *F Ras85DSutor-404 consists of a 5' splice site point mutation in intron 1, *F which changes the conserved GU to AU. The altered 5' splice site may no *F longer serve as a docking site for the U1snRNP complex of the spliceosome, *F and subsequently lead to incomplete RNA splicing. *F At your earliest convenience, please include this Molecular data into the *F FlyBase Report of these alleles. *F Thanks *F Toby # *U FBrf0125108 *a Xu *b E.Y. *t 2000.1.25 *T personal communication to FlyBase *u *F From eyxu@itsa.ucsf.edu Tue Jan 25 19:17:48 2000 *F To: Rachel Drysdale (Genetics) *F From: Eugene Yujun Xu *F Subject: Re: Helping FlyBase: xmas *F Dear Rachel, *F ... *F The xmas-1 and xmas-2 are two overlapping genes, xmas-1 being the *F 3kb transcript, corresponding the transcript g in Mckim et al. (1996). *F xmas-2 is a 5kb transcript proximal to xmas-1 but overlap with the first *F exon of xmas-1. *F The order of the genes in this region will be as follows: *F centromere ......bazooka xmas-2 xmas-1 M(1)15D mei218......telomere *F All the xmas alleles (xmasP, R1, R6 815-10 etc) affect both genes *F genetically only xmasP is known to disrupt xmas-1 transcript and insert a *F few bases upstream of xmas-2 start codon. *F I was slow in publishing the data, which result in such confusion. *F If you have any further questions feel free to contact me. Otherwise you *F should be able to see the papers on xmas soon as I am about to submit them *F now. *F best *F \-Eugene # *U FBrf0125109 *a White *b K.P. *t 2000.1.28 *T personal communication to FlyBase *u *F From kpwhite@cmgm.stanford.edu Fri Jan 28 20:58:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Jan 2000 20:58:00 +0000 *F X-Sender: kpwhite@cmgm.stanford.edu *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.1 *F Date: Fri, 28 Jan 2000 13:01:20 -0800 *F To: Gillian Millburn (Genetics) *F From: 'Kevin P. White' *F Subject: Re: FlyBase query *F Mime-Version: 1.0 *F Content-Type: multipart/alternative; boundary='=====================_169543306==_.ALT' *F Content-Length: 6746 *F Gillian, *F I just re-did a BLAST on that clone (HL02324), and it turns *F out not to be Mlc-k but instead bt a projectin. The error appears to be *F carried *F over from annotation assigned to this EST by the BDGP last year. Their records *F and those in my database are now updated. Thanks for pointing this out. *F (Incidentally, the number 2 BLAST hit still comes up as a Myosin LCK). If you *F are looking at any other ESTs from our paper (there were over 500 that we *F called significantly regulated during the onset of metamorphosis), I encourage *F you to re-BLAST them as well since updates to genbank come fast and furious. *F Kevin *F At 11:22 AM 1/28/00 +0000, you wrote: *F >Dear Dr. White, *F > *F >a couple of weeks ago I sent you a query regarding your paper White et *F >al., 1999, Science 286(5447): 2179--2184. I was wondering if you have *F >had a chance to look at it yet. I include a copy of the original *F >e-mail below: *F > *F >Gillian *F > *F >> From gm119@gen.cam.ac.uk Tue Jan 11 12:03:30 2000 *F >> Envelope-to: gm119@gen.cam.ac.uk *F >> Delivery-date: Tue, 11 Jan 2000 12:03:30 +0000 *F >> To: kpwhite@cmgm.stanford.edu *F >> Subject: FlyBase query *F >> Cc: gm119@gen.cam.ac.uk *F >> X-Sun-Charset: US-ASCII *F >> From: Gillian Millburn (Genetics) *F >> Date: Tue, 11 Jan 2000 12:06:40 +0000 *F >> Content-Length: 862 *F >> *F >> Dear Dr. White, *F >> *F >> I am curating your paper for FlyBase: *F >> *F >> White et al., 1999, Science 286(5447): 2179--2184 *F >> *F >> and I have a question about Figure 3B. *F >> *F >> One of the genes in this figure is given as 'myosin LCK' with the clone *F >> ID 'HL02324'. Could you confirm whether the 'myosin LCK' gene in your *F >> figure is the one we have in FlyBase as 'Mlc-k' or 'Myosin light chain *F >> kinase' which maps to 52D, or whether it is a different gene, *F >> *F >> I look forward to hearing from you, *F >> *F >> Gillian *F >> *F >> -------------------------------------------------------------- *F >> Gillian Millburn. *F >> *F >> FlyBase (Cambridge), *F >> Department of Genetics, *F >> University of Cambridge, *F >> Downing Street, email: gm119@gen.cam.ac.uk *F >> Cambridge, CB2 3EH, Ph : 01223-333963 *F >> UK. FAX: 01223-333992 *F >> -------------------------------------------------------------- *F >> *F \---------------------------------------------------------------------------- *F \-------------------------------------------------------- *F Kevin P. White *F Dept. of Biochemistry and *F Stanford DNA Sequencing and Technology Center *F 855 California Ave. *F Palo Alto, CA 94306 *F USA *F Ph: (650) 812-2008 *F FAX: (650) 812-1975 *F http://cmgm.stanford.edu/~kpwhite/ # *U FBrf0125110 *a Finkelstein *b R. *t 2000.1.31 *T personal communication to FlyBase *u *F From: Kevin Cook, Bloomington Stock Center *F Subject: Information from Bob Finkelstein *F The following information accompanied stocks donated by Bob Finkelstein, University of Pennsylvania, in November, 1999. *F 1. P{w[+mC]=oc-lacZ.7.6}QG1 insertion identifier *F The P{oc-lacZ.7.6} construct and its transformation were described in Gao *F and Finkelstein, 1998, Development 125(21): 4185--4193 (FBrf0104919). *F This third chromosome insertion was isolated from that study. The insertion *F chromosome is lethal, but whether the insertion is responsible is unknown. *F 2. P{w[+mC]=hsp70-otd.R}5A and P{w[+mC]=hsp70-otd.R}23C *F The P{hsp70-otd.R} construct and its transformation were described in Royet *F and Finkelstein, 1995, Development 121(11): 3561--3572 (FBrf0084326). The *F 5A insertion is on the third chromosome and the 23C insertion is on the X *F chromosome. Both insertions are homozygous viable and fertile. *F 3. l(2)31Ef[G78] and l(2)31Ef[E1-19] *F These are two of the three l(2)31Ef alleles described in Clegg et al., *F 1993, Genetics 134(1): 221--230 (FBrf0058585). No allele names were given *F in the paper. These two alleles came from the EMS screen described in the *F paper. *F 4. P{w[+mC]=UAS-oc} construct and insertions *F This construct, also known as P{UAS-otd}, and its transformation have not *F been published. The construct is a wild type ocelliless cDNA cloned into *F P{UAST}. A second and a third chromosome insertion were isolated. Both *F are homozygous viable and fertile. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0125111 *a Gornek *b M.A. *c K.P. *d Bogart *e K.R. *f Cook *t 2000.2.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Feb 04 16:39:24 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Dp(1;Y)s for the 10C to 11D Region *F Isolation and Characterization of Duplications of the 10C to 11D Region of *F the Drosophila X chromosome *F Millicent A. Gornek, Kevin P. Bogart and Kevin R. Cook *F Bloomington Drosophila Stock Center *F Indiana University *F Here we describe a screen for Y-linked duplications of the X chromosomal *F region distal to 11D3-8. *F In(1)sc260-14 with breakpoints 1B2-3;11D3-8 was recombined onto C(1;Y)6 *F to create C(1;Y)6, In(1)sc260-14. An attached-X stock of C(1;Y)6, *F In(1)sc260-14 was generated that lacked a free Y chromosome. Males from *F this stock were irradiated at 4000 rads using a cesium source and mated to *F homozygous y1 w1 females. 93,000 progeny (54,000 males) were screened *F for the presence of y+ w- males. Stocks were established by crossing *F these males to y1 w1 females. Ten out of thirty-nine y+ w- males *F were fertile and were used to make stocks. *F The screen was designed to recover deletions within C(1;Y)6, *F In(1)sc260-14 which extend from some point distal to the 11D3-8 *F breakpoint to X centric heterochromatin yielding a Dp(1;Y) chromosome with *F the following structure: the X tip to 1B1,2; a segment from 11D3-8 to a *F random breakpoint in region 10 or 11; a portion of X heterochromatin; a Y *F chromosome. The XLt to 1B1,2 segment carries a wild type allele of y, which *F allows the Dp(1;Y) chromosomes to be followed in crosses. *F Since the breakpoint of interest in these Dp(1;Y) chromosomes is juxtaposed *F to centric heterochromatin, determining precise breakpoints is impossible *F cytologically. Nevertheless, the bands visible in polytene preparations *F gave minimal extents of the duplicated regions: *F Dp(1;Y)BSC1 10C1,2;11D3-8 *F Dp(1;Y)BSC2 10E1,2;11D3-8 *F Dp(1;Y)BSC3 11B1,2;11D3-8 *F Dp(1;Y)BSC4 11B1,2;11D3-8 *F Dp(1;Y)BSC5 11B18;11D3-8 *F Dp(1;Y)BSC6 11B18;11D3-8 *F Dp(1;Y)BSC7 11B18;11D3-8 *F Dp(1;Y)BSC8 11C3-4;11D3-8 *F The polytene bands closest to the chromocenter in squashes appear to be *F from region 10 or 11. *F More precise placement of breakpoints for some, but not all Dp(1;Y) *F chromosomes came from comparing our cytology to information about the *F rescue of recessive mutations in regions 10 and 11 by the Dp(1;Y) chromosomes. *F Dp(1;Y)BSC1 rescued *F P{lacW}l(1)G0241G0241 10D1-3 *F P{lacW}l(1)G0102G0102 10E1-4 *F In(1)m38c, m38c 10E1-2;13B4-5 *F dy1 10E3-4 *F P{lacW}l(1)G0111G0111 10E *F qs1 10F1-7 *F fw34e 11A4 *F tsg2 11A2 *F P{lacW}l(1)G0060G0060 11B14-C2 *F We found no mutations distal to l(1)G0241 rescuable by Dp(1;Y)BSC1. The *F chromosomes with lethal mutations in 10BC we tested likely carried *F additional lethals. In this case, complementation tests did not allow us to *F refine the cytology. We suggest 10C1,2;11D3-8 as the provisional cytology *F of Dp(1;Y)BSC1 until a more precise distal breakpoint can be determined. *F Dp(1;Y)BSC2 rescued *F P{lacW}l(1)G0102G0102 10E1-4 *F In(1)m38c, m38c 10E1-2;13B4-5 *F dy1 10E3-4 *F P{lacW}l(1)G0111G0111 10E *F qs1 10F1-7 *F fw34e 11A4 *F tsg2 11A2 *F P{lacW}l(1)G0060G0060 11B14-C2 *F but did not rescue *F P{lacW}l(1)G0241G0241 10D1-3 *F These complementation tests are consistent with our cytological analysis *F which showed a breakpoint distal to 10E1,2, but do not provide any *F additional information for refining the position of the distal breakpoint. *F We suggest using 10E1,2;11D3-8 as provisional breakpoints for Dp(1;Y)BSC2. *F Dp(1;Y)BSC3, Dp(1;Y)BSC4, Dp(1;Y)BSC5, Dp(1;Y)BSC6, Dp(1;Y)BSC7 and *F Dp(1;Y)BSC8 all rescued P{lacW}l(1)G0060[G0060] (11B14-C2), but not tsg *F (11A2) or more distal mutations. This is consistent with the cytology of *F Dp(1;Y)BSC3 and Dp(1;Y)BSC4, but does not refine their distal 11B1,2 *F breakpoints; we suggest provisional breakpoints of 11B1,2;11D3-8. These *F complementation results allowed a more accurate placement of the distal *F breakpoints of Dp(1;Y)BSC5, Dp(1;Y)BSC6, Dp(1;Y)BSC7 and Dp(1;Y)BSC8; we *F suggest provisional breakpoints 11B14-C2;11D3-8. *F Two Dp(1;Y) chromosomes, Dp(1;Y)BSC9 and Dp(1;Y)BSC10, were recovered *F which appear to carry only X tip material; they retained the 1B1,2 *F band, but no bands from region 11 were apparent. They did not rescue *F P{lacW}l(1)G0060[G0060] (11B14-C2), the mutation closest to the 11D3-8 *F breakpoint in In(1)sc[260-14]. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0125112 *a Welte *b M. *t 2000.2.23 *T personal communication to FlyBase *u *F Date: Wed, 23 Feb 2000 10:54:03 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Region 22A lethals *F From: Kevin Cook, Bloomington Stock Center *F Subject: Region 22A lethals *F The following information accompanied stocks sent by Michael Welte from *F Eric Wieschaus' lab at Princeton (January 2000). *F Marya Postner screened for EMS-induced lethals that fall between the *F proximal breakpoints of Df(2L)S2 and Df(2L)S3. The mutations were induced *F in a cn1 bw1 sp1 chromosome. *F New locus name Original complementation group name Allele *F l(2)22Af T M260A *F lea lea M8 *F RFeSP H M73 *F l(2)22Ag A M1 *F l(2)22Ah B M11 *F l(2)22Ai D M16 *F l(2)22Aj E M105 *F l(2)04111 F M33 *F cpb J M143 *F l(2)22Ak G M101 *F Further deficiency mapping indicated the following order: *F distal (l(2)22Af) (lea) (RFeSP) (l(2)22Ag, l(2)22Ah, l(2)22Ai, l(2)22Aj, *F l(2)04111, cpb) (l(2)22Ak) proximal *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0125830 *a Nguyen *b D. *t 2000.1.20 *T personal communication to FlyBase *u *F From Duc Nguyen to Leyla Bayraktaroglu *F Jan 20, 2000 *F *F sidekick gene update: *F *F 1) EP(X)0369 insertion (accession # AQ025326) into exon 1 *F 2) additional introns found by comparison of cDNA sequence and the new *F genomic sequence from BDGP (clone BACR19J1, accession # *F AL132792), which tally up a total of 14 exons instead of the 10 exons *F described in the Development paper (FBrf0098319). *F 3) The 24 bp mini exon sequence is as follows: *F 5' AAA AAG GAG AGC CGT CAT TTG TTT 3' # *U FBrf0126706 *a van der Leij *b F.R. *t 2000.4.7 *T personal communication to FlyBase *u *F >From f.r.van.der.ley@med.rug.nl Fri Apr 07 22:51:15 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 22:51:15 +0100 *F Date: Fri, 07 Apr 2000 23:47:59 +0200 *F From: feike *F X-Mailer: Mozilla 4.5 [nl] (Win95; I) *F X-Accept-Language: nl *F MIME-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F Subject: cpt1 *F Content-Type: multipart/alternative; boundary='------------CBA3F3E8546ADE5181178610' *F X-Status: *F Content-Length: 1239 *F *F Hi *F *F Could you please correct the subcellular location assigned to CPT I *F (carnitine palmitoyltransferase I)? *F *F It is not in the mitochondrial inner membrane, but in the outer (cpt II *F is in the inner, but has not yet been functionally identified) *F *F http://flybase.bio.indiana.edu/.bin/fbidq.html?FBgn0027842 *F *F thank you *F *F Feike van der Leij, PhD *F Univ. Groningen, the Netherlands # *U FBrf0126707 *a Bayraktaroglu *b L. *t 2000.4.3 *T personal communication to FlyBase *u *F kn is CG10197 *F *F cas is CG2102 *F *F Msp-300 corresponds to parts of CG18252 and CG18251 (ie the two CGs will *F have to be merged and the structure fixed.) *F *F Ady43A is CG1851. *F *F Mcr is CG7586 (order from Crowley et al: Mcr- Trf+ Trfp-; Trfp is CG18267) *F *F fok (flegdling of Klp38B) mRNA has only very short putative ORFs that *F are possibly untranslated (but we do have a SEAN for it). *F *F Eip63F-2: it's not clear if it encodes a protein (putative 43 aa ORF.) *F *F Rbp's were short mRNAs with <50 amino acid CDS. # *U FBrf0126708 *a Bayraktaroglu *b L. *t 2000.4.7 *T personal communication to FlyBase *u *F Category 1: genes that we have no clue about - no sign of them in any jam *F page, no mention anywhere. *F *F Leyla's method: BLASTP (at BDGP) of entry in aa_embl_dros against the *F Predicted proteins (AA) set. Check match. If any ambiguity, do BLASTN *F at NCBI using relevant gene accession against scaffold accessions. *F If necessary view pairwise alignments using the SEAN tool (especially *F useful for aligning e.g clusters like and Met75Ca and Met75Cb annotated *F on genomic DNA with scaffold accessions.) *F *F *F Genes to which a CG can be assigned: *F *F Amy-p(FBgn0000079)=CG18640|FBan0018640|CT42306| *F *F BcDNA:GH03186(FBgn0028502)=CG6446|FBan0006446|CT20096| *F *F Cyt-P450-rBF6-2(FBgn0013773)=CG10240 *F (invalid gene symbol in GadFly Cyp6a22) *F *F EG:190E7.1(FBgn0024368)=CG18091|FBan0018091|CT40606|FBan0018091 *F *F EG:49E4.1(FBgn0025392)=CG3064|FBan0003064|CT10298| *F (intron-exon structure has to be corrected) *F *F Grip75(FBgn0026431)=CG6176|FBan0006176|CT19386|FBan0006176 *F *F Gtp-bp(FBgn0010391)=CG2522|FBan0002522|CT3545|FBan0002522 *F *F l(2)03659(FBgn0010549)=CG11803|FBan0011803|CT8643|FBan0011803 *F *F l(2)k10201 (FBgn0016970) =CG8803|FBan0008803|CT8653| *F (translation starts further upstream w/ CTG start than annotated on CG8803) *F *F l(2)tid(FBgn0002174)=CG5504|FBan0005504|CT17450|FBan0005504 *F (note: CG5504 is erroneously annotated as l(2)dtl in public view of GadFly; *F CG11295 is correctly annotated as l(2)dtl) *F *F Met75Cb(FBgn0028415)=CG18064|FBan0018064|CT40477|FBan0018064 *F (note:CG18064 is incorrectly identified as Met75Ca; sequence *F corresponding to Met75Ca exists but is unannotated. This is by *F alignment of Acc.AJ249253 (which has both genes) to AE003520.) *F *F msopa (FBgn0004414)=CG14560|FBan0014560|CT34291 *F (several indels give rise to frameshift in CG protein product.) *F *F Mst98Ca (FBgn0002865)=CG11719|FBan0011719|CT5056| *F *F Mst98Cb (FBgn0004171)=CG18396|FBan0018396|CT41800| *F *F Nckx30C (FBgn0028704)=CG4106|FBan0004106|CT13634| *F *F prc (FBgn0028573)=CG5700|FBan0005700|CT17960|FBan0005700 *F (intron-exon structure has to be corrected) *F *F Pros&agr;1 (FBgn0026781)=CG18495|FBan0018495|CT42044|CT42048| *F (note the 2 transcripts) *F *F Sgs7 (FBgn0003377)=CG18087|FBan0018087|CT40586| *F *F spen (FBgn0016977)=CG18497|FBan0018497|CT36331|CT42170| *F (CT36331 translation is very extremely truncated wrt known CDS, Missing *F from GadFly public view) *F *F ______________________________________________________________________________ *F Not as good a match as above, but good bet; save for reannotation if you wish: *F *F IM2 (FBgn0025583) is most likely CG18106|FBan0018106|CT40691 despite indel *F that causes difference in the middle of protein wrt translation of AF074003. *F This is one of 3 closely related genes close to each other (on AE003799.) *F *F ______________________________________________________________________________ *F Genes where there is a sequence match that is not annotated: *F *F BcDNA:GH02384 (FBgn0027612) there is matching sequence but is not annotated *F (matches AE003666: 223903(ATG)-222438(TAA) several exons; should be between *F CG11017 and CG2508) *F *F Orf5C (FBgn0011819) there is matching sequence but is not annotated *F (Orf5C is adjacent to Act5C) *F *F Ste (FBgn0003523) there is matching sequence but is not annotated *F (it should be in region next to ben (ben correctly annotated as CG18319 but *F no neighbors showing in GadFly; note:'Ste locus contains two major size classes *F of a tandemly repeated gene'; the 2 accessions in FlyBase align near each other) *F *F unknown-telomeric-protein-gene FBgn0027101 there is matching sequence but is not *F annotated *F (AF103941 aligns with coordinates 42123-42832 of AE003163) *F ______________________________________________________________________________ *F Ambiguous (no good GadFly BLAST hits, no good uninterrupted BLASTN alignment *F to NCBI contigs, should be rechecked when reannotating) *F *F Crg-1 no CG annotated *F 3' end of Crg-1 transcripts align to Acc. AE003327 *F (this is a tiny contig) *F *F *F Dbp80 no CG annotated *F *F BLAST hits fall on 3 short scaffolds (AE003058, AE002987, AE003078) *F 1 scaffold has annotation for another gene, other 2 have no annotations *F *F Lcp6 no unambiguous matches *F *F (note this from FB and paper:The Lcp6 protein may be encoded by an extra *F copy of the 'Lcp-b' gene (Lcp65Ab3) present in the Canton S strain. *F i.e. it may not be in all strains.) *F *F _____________________________________________________________________________ *F Genes where the symbol has changed (valid symbol already has a CGnumber): *F *F BcDNA:GH03016 is a synonym of Rh50 CG7499 *F BcDNA:GH04413 is a synonym of Appl CG7727 *F BcDNA:GH08312 is a synonym of Sap-r CG12070 *F _____________________________________________________________________________ *F Merges: *F Ser5 is probably a fragment of Ser99Da (Ser5 and Ser6 are PCR fragments). *F (Will send alignment separately to MA and AG) # *U FBrf0126709 *a Bayraktaroglu *b L. *t 2000.4.8 *T personal communication to FlyBase *u *F Category 2: genes that are known to be in a region where there are no CG's *F (often an intron of another gene). Also a few cases where there is a CG in *F the right place but there's more than one gene to match to it. If no *F CT/CG match can be made then log it for the time of reannotation. *F *F Leyla's method: BLASTP at Berkeley using FASTA from aa_embl.dros or aa_Adh.dros. *F If no good hits, BLASTN at NCBI using FASTA from na_embl.dros or na_Adh.dros *F (or FASTA from NCBI accession: e.g. EG:EG0007.5 translation missing from *F aa_embl.dros due to formatting glitch.) *F Alignments with SEAN as needed for verification. *F *F *F MATCHES: *F *F Bem46 FBgn0025109=CG18642|FBan0018642|CT42330| *F (adjacent to CG3736 okr) *F *F BG:DS00810.3=CG18456|FBan0018456|CT35228| *F *F BG:DS05899.7(548aa)=CG18095|FBan0018095|CT40625|(442) *F CG18095 different after aa 416 and truncates at aa 442. *F *F BG:DS07473.1(2190aa)=CG12455|FBan0012455|CT32536|(2172aa) *F Mostly correct! CG12455 missing 18 aa between aa 84-102 of published *F CDS. *F *F BG:DS07721.3=CG15282|FBan0015282|CT35230 *F aa 1-139 of CG15282 match aa 54-192 of BG:DS07721.3 from aa_Adh.dros. *F (but structure needs to be checked.) *F *F *F SPLIT between genes, to be merged/fixed: *F *F BG:DS00180.5: (no CG between BG:DS00180.3 and BG:DS00180.12) *F some of BG:DS00180 (734 aa) is split between CG8855|FBan0008855|CT25428| (832aa) *F (aa 394-620 align) and CG17988|FBan0017988|CT40163| (792aa) (aa 166-282 align) *F No good BLASTP match to the rest of the protein. *F *F *F PART OF HYBRID, to be split: *F *F BG:DS00180.7: *F CG16882|FBan0016882|CT37468(1035aa)|CT37470|(917aa) is a hybrid between *F BG:DS00180.7 (421aa) and BG:DS00180.8. (448 aa) *F Note two transcripts associated with CG16882: *F aa 8-421 of BG:DS00180.7 correspond to aa 504-917 of CT37470 and *F aa 622-1035 of CT37468 *F aa 1-448 of BG:DS00180.8 correspond to aa 1-448 of CT37468 *F aa 119-448 of BG:DS00180.8 correspond to aa 1-330 of CT37470 *F *F EG:34F3.2: *F CG12467|FBan0012467|CT32681| (1376aa) is a hybrid of EG:34F3.2 FBgn0025622 *F (1014aa) and EG:34F3.1 FBgn0025623 (504 aa) *F *F EG:EG0007.4: *F CG4857|FBan0004857|CT15601| (1911aa) is a hybrid of *F EG:EG0007.4 FBgn0026082 (911 aa) and EG:EG0007.12 FBgn0026083 (978 aa) *F *F *F ONE gene with 2 transcripts, one transcript corresponds to *F EG:140G11.3, the other corresponds to FcpC3!: *F *F EG:140G11.3=G4015|FBan0004015|CT41858| *F FcpC3=CG4015|FBan0004015|CT13332| *F *F *F TO BE ANNOTATED in the future, but the presence of annotatable sequence has *F been verified by BLASTN to scaffolds at NCBI: *F *F gdl-ORF39 no CG between Z600 and Eip71CD *F (aligns to AE003530) *F (end of gdl-ORF is only 68 bp from gdl ATG) *F *F l(2)rot no CG within l(2)not *F (aligns to AE003461) *F *F Lcp65Ab1 no CG between Lcp65Aa and Lcp65Ac *F (aligns to AE003563) *F *F Lcp65Ab2 no CG between Lcp65Aa and Lcp65Ac *F (aligns to AE003563) *F *F scw no CG within Lar *F (aligns to AE003663) *F (NB:translation of Lar CG10443|FBan0010443|CT29268| is severely *F truncated: 2029 aa in CDS set vs 74 aa from CT29268!) *F (scw should be between exon 4 and 5 of Lar) *F *F BG:DS02795.3 only CG12455/CT32536 between PRL-1 and Ca-&agr;1D *F (aligns to AE003650) *F *F BG:DS00810.1 only CG18456/CT35228 between BG:DS07721.6 and BG:DS06874.1 *F (aligns to AE003645) *F *F BG:DS00810.2 only CG18456/CT35228 between BG:DS07721.6 and BG:DS06874.1 *F (aligns to AE003645) *F *F BG:DS00929.15 no CG between vig and vas *F (aligns to AE003646) *F *F BG:DS01514.3 no CG between rk and bgm *F (aligns to AE003642) *F *F BG:DS03192.3 no CG between stc and BG:DS03192.2 *F (aligns to AE003646) *F *F BG:DS03192.4 no CG within BG:DS03192.2 *F (aligns to AE003646) *F *F BG:DS03792.2 no CG within wb aka CG15288/CT35236 *F (aligns to AE003643) *F *F BG:DS05899.6 only CG18095/CT40625 between BG:DS05899.1 and BG:DS05899.3 *F (aligns to AE003642) *F *F BG:DS07295.4 no CG within BG:DS07295.1 *F (aligns to AE003646) *F *F BG:DS07721.1 only CG15282/CT35230 between Adhr and BG:DS07721.6 *F (aligns to AE003644) *F *F BG:DS08249.1 only CG18507/CT42208 (nj) between BG:BACR48E02.4 and BG:DS08249.2 *F (aligns to AE003641) *F *F BG:DS08249.4 no CG between BG:DS08249.3 and BG:DS00797.1 *F (aligns to AE003641) *F *F BG:DS08249.5 no CG between BG:DS08249.3 and BG:DS00797.1 *F (aligns to AE003641) *F *F BG:DS09219.1 only CG15282/CT35230 between Adhr and BG:DS07721.6 *F (aligns to AE003644) *F *F EG:EG0007.5 no CG between EG:EG0007.12 and Fas2 *F (aligns to AE003430) # *U FBrf0126710 *a Misra *b S. *t 2000.4.5 *T personal communication to FlyBase *u *F Category 3: genes with >=two candidate CG's between their neighbouring *F genes; analyzed by taking translations from Adh region (aa_Adh.dros) and *F translations from GadFly (aa_gadfly.dros) and using ClustalW1.8; where *F necessary, using BLASTP against aa_gadfly.dros first (when gene did not *F correspond to either candidate CG) *F *F >beat-B=CG7644|FBan0007644|CT23325 *F missing 1-9aa and last 232-326aa *F not CG13244|FBan0013244|CT32494 *F *F >beat-C=CG4838|FBan0004838|CT15531 *F missing 1-15aa, including new 5aa at ~aa50, new 4aa at ~aa130 *F not CG13245|FBan0013245|CT32496 *F *F >BG:DS00365.2(1269aa)=CG18096|FBan0018096|CT40635(583aa) *F missing aa583-1269 *F not CG18629|FBan0018629|CT41822 *F *F >BG:DS00929.6(1713aa)=CG15274|FBan0015274|CT35221(1713aa) *F looks right *F not CG4182|FBan0004182|CT12287 or CG4180|FBan0004180|CT13516B *F *F >l(2)35Bg(248aa)=CG4180|FBan0004180|CT13516(248aa) *F looks right *F not CG4182|FBan0004182|CT12287 *F *F >BG:DS03023.4(1073aa)=CG18482|FBan0018482|CT42130(906aa) *F missing aa508-612, aa799-860 *F not CG15258|FBan0015258|CT35202 *F *F >BG:DS03144.1(715aa)=CG15276|FBan0015276|CT35223(227aa) *F missing aa1-280, aa508-715 *F not CG15277|FBan0015277|CT35224 *F *F >BG:DS06874.4(600aa)=CG15280|FBan0015280|CT35227(272aa) *F missing aa1-328 *F not CG4650|FBan0004650|CT14964 *F *F >BG:DS06874.6(299aa)=CG18420|FBan0018420|CT40737(292aa) *F missing aa1-7(this could be right) *F not CG18636|FBan0018636|CT42230 (nj) or CG15280|FBan0015280|CT35227 *F *F >BG:DS06874.7(706aa)=no gene in GadFly *F not CG18636|FBan0018636|CT42230 (nj) or CG18420|FBan0018420|CT40737 *F *F >BG:DS07486.3(781aa)=CG4793|FBan0004793|CT15407(875aa) *F G4793 has extra 5aa at beginning (not M), extra ~100aa at end *F not CG13244|FBan0013244|CT32494 *F *F >l(2)35Fb(503aa)=CG4163|FBan0004163|CT13756(503aa) *F looks right *F not CG13258|FBan0013258|CT32541 # *U FBrf0126711 *a Misra *b S. *t 2000.4.6 *T personal communication to FlyBase *u *F Category 4: genes that were in the penultimate XML but are now gone and *F for which I can't find a replacement in the final xml (but I did no seq *F alignment). *F *F Sima checked these by BLASTing protein sequence from aa_embl.dros at the *F BDGP against the latest version of aa_gadfly.dros. Those that did not *F match, I tried the nucleic acid sequence from na_embl.dros against *F na_gadfly.dros and then against genomic scaffolds at NCBI. *F *F *F >&bgr;ggt-II(347aa) *F =CG18627|FBan0018627|CT41437|FBan0018627 last_updated:000321(347aa) *F CT10156 == CG3023, gone *F *F >A(225)(711aa) *F =CG18255|FBan0018255|CT41348|FBan0018255 last_updated:000321(1277nt) *F (only found as nucleic acid, not as protein) *F CG8307 no jam, gone *F *F >Act87E(376aa) *F =CG10067|FBan0010067|CT28343|FBan0010067 last_updated:000321(376aa) *F CT34012 no CG and CT26437 CG9278 gone *F *F >Aprt(182aa) *F =CG18315|FBan0018315|CT41563|FBan0018315 last_updated:000321(182aa) *F CG1833 CT5556, gone *F *F >axo(1685aa) *F =CG18296|FBan0018296|CT41507|FBan0018296 last_updated:000321(482aa) *F CG7517 CT1277, gone *F *F >Bbbf1(638aa) =no gene in GadFly but matches Celera AE003608 (repeated *F many times in this scaffold sequence) *F CT39704 == CG15836, gone *F *F >BG:DS01368.1(514aa) *F =CG18507|FBan0018507|CT42208|FBan0018507 last_updated:000321(463aa) *F CG7153 CT22111 gone *F *F >Eip93F(1221aa) *F =CG18389|FBan0018389|CT41792|FBan0018389 last_updated:000321(1075aa) *F CG6505 CT20215, gone *F *F >Gprk1(700aa) *F =no gene in GadFly but matches Celera AE002943, AE002613, AE002814, *F AE003008, AE003017 (split among many scaffolds) *F CT39791 == CG17882 gone *F *F >Optix(60aa) *F =CG18455|FBan0018455|CT42028|FBan0018455 last_updated:000321(218aa) *F CG8718 CT9483, gone *F *F >Os9(159aa) *F =CG10658|FBan0010658|CT29854|FBan0010658 last_updated:000321 *F (only found as nucleic acid, not as protein) *F CG10656 CT29836, gone *F *F >Shark(950aa) *F =CG18247|FBan0018247|CT41326|FBan0018247 last_updated:000321(939aa) *F CG5801 and CG11991 both gone # *U FBrf0126712 *a Misra *b S. *t 2000.4.8 *T personal communication to FlyBase *u *F Category 5: genes that have a jam page but it doesn't give a CG, and *F for which I can't find a replacement in the final xml *F *F Sima analyzed by taking fasta of protein from aa_embl.dros and BLASTing at *F BDGP against aa_gadfly.dros. Those that did not match, I tried the *F nucleic acid sequence from na_embl.dros against na_gadfly.dros and then *F against genomic scaffolds at NCBI. *F *F *F >BcDNA:LD22218(4601bp) *F emb|AF132192|AF132192 BcDNA:LD22218 FBgn0027510 AF132192:117..4367 *F =no gene in GadFly, but matches Celera AE003809 63060-63161, 63247-65728, *F 65786-66076, 66164-67544 (no gene annotated at these coordinates) *F CT21748 no CG *F *F >bl(490aa) *F emb|AF142631|AF142631 bancal FBgn0015907 AF142631:220..1692 *F =CG13425|FBan0013425|CT40495|FBan0013425 last_updated:000321(499aa) *F CT32782 with no CG *F *F >Eip63F-1(582bp) *F emb|U25882|DM25882_2 Eip63F-1 FBgn0004910 SWISS-PROT:P48593 *F U25882:359..940 *F =CG15855|FBan0015855|CT40256|FBan0015855 last_updated:000321(222bp) *F matches Celera AE003479 246905-246870, 244623-244581, 244479-244436, *F 233654-233506, 227596-227440, 227366-227214, 225503-225480 *F CT36601 with no CG *F *F >Gprk2(714aa) *F emb|AF004674|DMAF4674_2 Gprk2 FBgn0004834 AF004674:1119..326 *F =CG17998|FBan0017998|CT40234|FBan0017998 last_updated:000321(714aa) *F CT4320 with no CG *F *F >Klp54D(133aa) *F emb|M74427|DMKINA Klp54D FBgn0004377 SPTREMBL:Q00082 M74427:1..399 *F =CG15844|FBan0015844|CT40108|FBan0015844 last_updated:000321(844aa) *F CT15768 no CG *F *F >lt(841aa) *F emb|AF034571|AF034571 lt FBgn0002566 AF034571:83..2608 *F =CG18028|FBan0018028|CT40348|FBan0018028 last_updated:000321(841aa) *F CT38727 with no CG *F *F >LysA(140aa, 423nts) *F emb|Z22223|DMLYSPREA_2 LysA FBgn0011201 SWISS-PROT:P37157 Z22223:2..424 *F =CG9118|FBan0009118|CT9902|FBan0009118 *F last_updated:000321(423nt)(406/409nt ids) *F CT9339 with no CG *F [note that very similar gene is *F >LysE *F emb|AC005847|FBpp0000780 LysE FBgn0004428 FLYBASE:FBpp0000780 AC005847:4355..4777 *F =CG1179|FBan0001179|CT2078|FBan0001179 *F last_updated:000321(452nt)(406/423nt ids) *F (confirmed with CLUSTALW comparing all Lys gene nt sequences)] *F *F >M(2)21AB(405aa) *F emb|X77392|DMSAMS_2 M(2)21AB FBgn0005278 SWISS-PROT:P40320 *F X77392:232..1449 *F =CG2674|FBan0002674|CT31899|FBan0002674 last_updated:000321(408aa) *F =CG2674|FBan0002674|CT31907|FBan0002674 last_updated:000321(408aa) *F CT9065 with no CG *F *F >mys(846aa) *F emb|J03251|DMMYSP_3 mys FBgn0004657 SWISS-PROT:P11584 J03251:140..2680 *F =CG1560|FBan0001560|CT40473|FBan0001560 last_updated:000321(845aa) *F CT4046 with no CG *F *F >noe(74aa) *F emb|AF077736|AF077736 noe FBgn0026197 AF077736:269..493 *F =no gene in GadFly, but matches Celera AE003524 93261-93387, but no gene *F annotated at these coordinates (in intron of blot gene) *F CT40002 no CG *F *F >Rya-r44F(5141aa) *F emb|D17389|DMRYRH_2 Rya-r44F FBgn0011286 SPTREMBL:Q24498 *F =CG10844|FBan0010844|CT30357|FBan0010844 last_updated:000321(5107aa) *F CT25270 no CG *F *F >shi(883aa) *F emb|X59449|DMDDYN4_2 shi FBgn0003392 SWISS-PROT:P27619 X59449:52..2703 *F =CG18102|FBan0018102|CT40657|FBan0018102 last_updated:000321(819aa) *F CT35729 with no CG and CT25562 with no CG # *U FBrf0126717 *a Ahmad *b ?. *t 2000.3.13 *T personal communication to FlyBase *u *F From kahmad@fred.fhcrc.org Fri Mar 03 19:07:01 2000 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 3 Mar 2000 19:07:01 +0000 *F Date: Fri, 3 Mar 2000 11:10:51 -0800 (PST) *F From: Kami Ahmad *F X-Sender: kahmad@fred *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-245A *F MIME-Version: 1.0 *F dear rachel - it is a pleasure to help *F flybase, especially on the matter of the *F the gene cid (for centromere identifier) *F was reported in Henikoff, S., K. Ahmad, *F J. S. Platero, and B. van Steensel (2000). *F Heterochromatic deposition of centromeric *F histone H3-like proteins. PNAS 97:716-721. *F the gene sequence is contained within a *F Berkeley Genome Project sequence, AC005652, *F and is at 50A on the cytological map, between *F the arr and drk genes (defined by sequence *F analysis and more recently by complementation *F with deficiencies in the region - this latter *F stuff is not yet published). is this the info *F that you need? -yours, kami # *U FBrf0126721 *a Bello *b B. *t 2000.3.15 *T personal communication to FlyBase *u *F Delivery-date: Wed, 15 Mar 2000 13:57:42 +0000 *F Date: Wed, 15 Mar 2000 14:04:29 +0100 *F From: Bruno Bello *F Subject: Re: FlyBase Query (cy456) *F To: Chihiro Yamada *F Dear Chihiro, *F I have indeed made the UAS-Antp construct and flies cited in Kurata et al. *F For this I isolated an Antp cDNA of 2,2 kb as a NotI fragment from pNHTA *F (Gibson et al,1990, Cell 62: 1087-1103) and inserted it in the pUAST vector *F of Andrea Brand (Brand and Perrimon, 1993, Development 118:401-415) at the *F same site, orientation selected by restriction digest. As far as I am aware *F this is the first time this construct appears in a published paper. *F Please let me know if you need further informations. *F Best regards, *F Bruno *F Bruno Bello *F Mammalian Development *F National Institute for Medical Research *F London Mill Hill NW7 1AA *F Tel: 44 (0) 181 959 3666 ext 2118 *F Fax: 44 (0) 181 913 8543 *F e-mail: bbello@nimr.mrc.ac.uk *F From cy200@gen.cam.ac.uk Tue Mar 14 18:36:09 2000 *F Delivery-date: Tue, 14 Mar 2000 18:36:09 +0000 *F To: bbello@nimr.mrc.ac.uk *F Subject: FlyBase Query (cy456) *F From: Chihiro Yamada *F Date: Tue, 14 Mar 2000 18:40:19 +0000 *F Dear Dr Bello, *F I am currently curating a paper for FlyBase: *F Kurata et al., 2000, Proc. Natl. Acad. Sci. 97(5): 2117--2122 *F The authors in this paper have cited you as the source for the UAS-Antp *F strain of flies. I have a question about these flies was hoping you *F could answer for me: *F UAS-Antp *F There are three UAS-Antp alleles in our files. Is the one you gave to *F Kurata et al one of these? If it is not, or you are not sure, could *F you provide me with some details about this construct. Did you make *F this construct? What are the molcular details of this contruct? Are *F there any other papers that you know of that this construct has been *F used? *F AntpScer\UAS.cCa *F Roy et al., 1997, Curr. Biol. 7(4): 222--227 *F Saccharomyces cerevisiae UAS construct a of Carroll *F provided by Sean Carroll. *F in vitro construct | regulatory fusion *F P{UAS-Antp} *F AntpScer\UAS.cKa *F Heuer et al., 1995, Development 121(11): 3861--3876 *F Li and McGinnis, 1999, Proc. Natl. Acad. Sci. USA 96(12): 6802--6807 *F Yao et al., 1999, Dev. Biol. 211(2): 268--276 *F Saccharomyces cerevisiae UAS construct a of Kalkbrenner *F in vitro construct | regulatory fusion *F P{UAS-Antp.K} *F AntpScer\UAS.cMa *F Azpiazu and Morata, 1998, Genes Dev. 12(2): 261--273 *F Casares and Mann, 1998, Nature 392(6677): 723--726 *F Ryoo and Mann, 1999, Genes Dev. 13(13): 1704--1716 *F Saccharomyces cerevisiae UAS construct a of Moreno *F Gift from Ecuador Moreno. *F in vitro construct | regulatory fusion *F P{UAS-Antp.M} *F Thanks for your time and best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0126737 *a Meier *b P. *t 2000.4.5 *T personal communication to FlyBase *u *F From meierp@icrf.icnet.uk Wed Apr 05 14:34:00 2000 *F Delivery-date: Wed, 5 Apr 2000 14:34:00 +0100 *F Date: Wed, 5 Apr 2000 14:38:01 +0100 *F To: cy200@gen.cam.ac.uk *F From: Pascal Meier *F Dear Chihiro, *F .. *F So here are my answers to all of your questions: *F pUAST-pro-dronc: Complete coding sequence from nt 369 to1721 *F pUAST-(Delta)N dronc: Dronc without its prodomain; coding sequence from nt *F 706 to1721. *F pUAST-pro-dronc C->A: Complete coding sequence of dronc (from nt 369 *F to1721) but with the catalytic Cysteine mutated to Alanine (Aa *F substitution). This protein is a catalytically inactive version of dronc *F and does not induce cell death. *F pUAST-(Delta)N dronc C->A: Dronc without its prodomain; coding sequence *F from nt 706 to1721 but with the catalytic Cysteine mutated to Alanine (Aa *F substitution). This protein is a catalytically inactive version of dronc *F and does not induce cell death. *F pUAST-dronc-CARD: Prodomain of dronc on its own; coding sequence form nt *F 369 to 786. *F .. *F Yours sincerely *F Pascal *F Dr. Pascal Meier *F Biochemistry of the Cell Nucleus *F Laboratory *F Imperial Cancer Research Fund *F 44 Lincoln's Inn Fields *F London WC2A 3PX *F Tel.: 020 7269 28 38 *F Fax: 020 7269 32 30 *F e-mail: meierp@icrf.icnet.uk *F ================================================================================ *F Dear Dr Evan, *F I am currently curating your paper for FlyBase: *F Meier at al., 2000 EMBO J. 19:598--611. *F I have a few questions I was hoping you could answer for me. *F \--DRONC constructs *F You use a number of DRONC constructs which you insert into pUAST and transform into flies. Could you give me some more details on the molecular details of these constructs? e.g. Is it a full length construct, what fragment of the cDNA was used (or has been deleted) in each construct? *F You call them: *F pro-dronc *F (Delta)dronc *F pro-dronc C->A *F (Delta)dronc C->A *F dronc-card *F I hope to hear from you soon, *F Best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0126754 *a Fehon *b R. *t 2000.3.8 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Mar 07 12:16:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 7 Mar 2000 12:16:53 +0000 *F To: rfehon@duke.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 7 Mar 2000 12:20:59 +0000 *F Content-Length: 816 *F Dear Dr. Fehon, *F I am curating your paper for FlyBase: *F McCartney et al., 2000, Development 127(6): 1315--1324 *F and I have a question about the 'P{w+,UpMer+}' construct you used in *F the clone experiments. *F I would be grateful if you could tell me what promoter is driving Mer *F expression - is it the polyubiquitin promoter (I think that's what the *F 'Up' is for but I wasn't sure) *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From rfehon@duke.edu Tue Mar 07 13:09:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 7 Mar 2000 13:09:35 +0000 *F X-Sender: rfehon@mail-rf.acpub.duke.edu *F Mime-Version: 1.0 *F Date: Tue, 7 Mar 2000 08:12:14 -0500 *F To: Gillian Millburn (Genetics) *F From: Rick Fehon *F Subject: Re: FlyBase query *F Yes, this is correct. In addition, the polyadenylation signal from the Adh *F gene was used at the 3' end. *F Thank you for taking care of this. *F Rick Fehon *F >Dear Dr. Fehon, *F > *F >I am curating your paper for FlyBase: *F > *F >McCartney et al., 2000, Development 127(6): 1315--1324 *F > *F >and I have a question about the 'P{w+,UpMer+}' construct you used in *F >the clone experiments. *F > *F >I would be grateful if you could tell me what promoter is driving Mer *F >expression - is it the polyubiquitin promoter (I think that's what the *F >'Up' is for but I wasn't sure) *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- # *U FBrf0126770 *a Bloomington Drosophila Stock Center *b ?. *t 2000.3.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sat Mar 11 00:20:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 11 Mar 2000 00:20:53 +0000 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Version 4.3 *F Date: Fri, 10 Mar 2000 19:21:39 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GAL4-Hsp70.PB} in FBrf0112022 *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{GAL4-Hsp70.PB} in FBrf0112022 *F Joel Eissenberg, Washington University, confirmed (3/00) that the *F Hsp70-GAL4 construct used in Rudolph et al. 1999, D. I. S. 1999 *F 82:99--100 FBrf0112022 is P{GAL4-Hsp70.PB}. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0126771 *a Eeken *b J. *t 2000.3.10 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Mar 10 22:35:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 10 Mar 2000 22:35:01 +0000 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Version 4.3 *F Date: Fri, 10 Mar 2000 17:35:45 -0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Information from Jan Eeken *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Stock Center *F Subject: Information from Jan Eeken *F The following information accompanied stocks donated to the Stock Center *F (2/00) by Jan Eeken, Leiden University. *F 1. v54k *F This v allele is present on a rud1 v54k tc1 sl2 smd1 chromosome. *F 2. Df(1)BA2-8 *F This Df for 4F5;5A13 was isolated on a Basc chromosome after X-irradiating *F males on the basis of its failure to complement cx1. *F 3. Df(1)M38-C5 and yM38-C5 *F This Df for 8B;8E was isolated after X-irradiating spermatids in wild type *F males. It was isolated along with a new y2-like mutation, yM38-C5. *F 4. Df(1)vN124B *F This Df or 9E3-F3;10A1-8 originally came from Abe Schalet and was likely *F induced by neutron irradiation. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0126814 *a Bowman *b A. *t 2000.3.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Mar 30 17:11:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Mar 2000 17:11:45 +0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Version 4.3.1 *F Date: Thu, 30 Mar 2000 11:12:56 -0500 *F To: Gillian Millburn (Genetics) *F From: Kevin Cook *F Subject: Df(2R)robl-c *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Stock Center *F Subject: Df(2R)robl-c *F The following information accompanied a Df(2R)robl-c stock from Aaron *F Bowman (2/00) in the lab of Larry Goldstein, University of California at *F San Diego. *F Aaron wrote: *F 'As for the breakpoints; let me give you some background info to this line. *F Roblc was derived from a large mass 'local hop' experiment in which 5 *F p-element lines (l(2)k08901, l(2)k11012, l(2)k06904, l(2)k07433, *F l(2)k11303) from the robl region were 'hopped' (with delta2-3) in mass (in *F one large cross) then the progeny were outcrossed and checked for *F complementation against robl. roblc was one of two robl alleles derived *F from this scheme (the other is called roblb). I characterized both lines *F by p-element rescue, inverse PCR rescue, and southern analysis. roblc is *F a deficiency over the robl region; the right breakpoint we indicate in the *F paper flanks the 3' end of the pLacW element. I have been able to get inv. *F pcr rescue off of the 5' end to determine the left breakpoint, but the *F analysis is difficult to interpret because there appear to be at least 2 *F different 5' p-element ends (i.e. I got 2 different sequences). One of *F these sequences appears to be from the parental p-element, while the other *F is not found within the genomic region around robl that we sequenced. Thus *F my best guess is that we got a local hop which left a small portion of the *F 5' end of the parental p-element at the original locus; and then had an *F insertion event which deleted a region around and including robl. We were *F able to confirm that at least the region from the left breakpoint of *F roblk to the right breakpoint of roblc are deleted by southern *F analysis. This is where our analysis of the deficiency ended (since we only *F cared that it broke at least to the left of the roblk breakpoint. You can *F refer to the genomic map I provided in the paper to see what I'm talking *F about. roblb appears to be derived from l(2)k08901 (it fails to *F complement this and only this parental p-element line). The 5' rescues off *F of roblb match one of the 5' rescues off of roblc; thus I believe this *F may be the parental 5' end; and that both alleles are derived from *F l(2)k08901. However, roblc complements l(2)k08901; so at the very least *F the parental p-element is no longer intact in roblc. Thus if I were to *F guess, the left breakpoint of roblc falls somewhere between the *F l(2)k08901 and the left break of roblk.' *F Note that 'roblk' is Df(2R)k10408 and that the paper he refers to is *F Bowman et al. J. Cell Biol. 1999 146(1):165--180 FBrf0109386. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0126832 *a Beaton *b A. *t 2000.1.31 *T personal communication to FlyBase *u *F From abeaton@uclink4.berkeley.edu Mon Jan 31 20:13:33 2000 *F To: flybase-updates@morgan.harvard.edu *F Dear FlyBase, *F Hi. I hope the work on these Ps can be included in your records. *F Respectfully submitted, *F Amy Beaton *F for BDGP *F BDGP P element Update *F 49 P element strains were selected for excision work. Their genomic *F flanks associate them with ESTs that have homologies with interesting *F transcripts in other organisms. *F l(2)00734 57E3-4 *F l(2)01501 32D1-2 *F l(2)02516 48C1-2 *F l(2)03775 46D1-2 *F l(2)04524 42F1-2 *F l(2)06444 48E10-11 *F l(2)07838 55D1-2 *F l(2)09373 60B10-11 *F l(2)10408 35B8-9 *F l(2)k00103 51C1-2 *F l(2)k00311 31E1-2 *F l(2)k00619 21F1-2 *F l(2)k03902 36B1-2 *F l(2)k04810 53E1-2 *F l(2)k05103 47F8-9 *F l(2)k06103 47F1-2 *F l(2)k06109 42A1-2 *F l(2)k06709 31D8-9 *F l(2)k06710 36C8-11 *F l(2)k07736 23F5-6 *F l(2)k07918 22B6-7 *F l(2)k08819 36A12-14 *F l(2)k09003 25C1-2 *F l(2)k09217 52D11-12 *F l(2)k09514 44C1-2 *F l(2)k10617 27C6-8 *F l(2)k10712 49D5-6 *F l(2)k13807 32D4-5 *F l(3)00848 99F10-11 *F l(3)01031 94F1-2 *F l(3)01418 64C9-10 *F l(3)02267 84C1-2 *F l(3)04210 89A1-2 *F l(3)05146 97D3-6 *F l(3)05614 84A4-5 *F l(3)10547 73D1-2 *F l(3)j10B2 77B6-7 *F l(3)j1C7 66B10-11 *F l(3)j1E6 82A3-5 *F l(3)j2B8 67B10-11 *F l(3)j2D9 96B19-20 *F l(3)j3A4 82D1-2 *F l(3)j3B9 100B2-4 *F l(3)L2249 65E10-11 *F l(3)L4032 89D1-2 *F l(3)L4910 94B4-5 *F l(3)rK561 66D10-13 *F l(3)rL562 67E1-4 *F l(3)s1939 72D8-9 *F 5 independent sublines (single chromosomes) of each strain were *F outcrossed to either w1118 or ry506 for six generations before *F rebalancing crosses were made to reestablish the stocks from single *F chromosomes. *F The following strains were found to bear viable insertions and should *F be changed to no P phenotype. *F l(2)k04810 53E1-2 homozygotes (k10209 and k04810 inserted one base apart) *F l(2)k05103 47F8-9 homozygotes *F l(2)k07736 23F5-6 homozygotes \** see note below regarding background lethals *F l(2)k09217 52D11-12 homozygotes *F l(3)05146 97D3-6 homozygotes. It seems likely that the *F l(3)05146 stock I started with had a problem because there are *F multiple alleles of the locus and another one l(3)L1602 has remained *F lethal through outcrossing. *F One line was not recovered after outcrossing: *F l(3)rK561 66D10-13 Rebalancing of outcrossed males via e *F ftz ry506/TM3, ryRK resulted in no male, ry+/TM3 progeny in 4 of *F 5 sublines. The 5th subline gave a mess in the F2. *F The remainder of the lines were still lethal after outcrossing. *F Excision work proceeded with lines that were either single insert *F lines or were verified only by means of noncomplementation with a *F deficiency. 50 independent excision events were followed per line *F (with the exception of l(3)j1C7 which needed 100 lines to get 6 *F viable lines). The following lines were successfully reverted and the *F frequency of reversion is indicated *F l(2)00734 08.6% *F l(2)07838 66.7% *F l(2)09373 35% *F l(2)10408 61.1% *F l(2)k00311 77.6% *F l(2)k00619 65% *F l(2)k06103 74.4% *F l(2)k09514 27.7% *F l(2)k10712 50% *F l(2)k13807 74% *F l(3)01031 19.35% *F l(3)01418 54.83% *F l(3)04210 11.76% *F l(3)j10B2 67.85% *F l(3)j1C7 06% *F l(3)j1E6 15.15% *F l(3)j2B8 39.28% *F l(3)j2D9 69.23% *F l(3)j3A4 80.00% *F l(3)L4032 67.56% *F l(3)L4910 18.75% *F l(3)rL562 44.73% *F Rebalancing of excision chromosomes failed to produce homozygous *F viable chromosomes in the following lines. *F l(2)k07918 no target site duplication *F l(3)L2249 *F l(3)s1939 *F l(3)02267 no target site duplication *F l(3)s1939 had 26 excisions and none produced homozygous viable chromosomes. *F l(3)02267 had 29 excisions and none produced homozygous viable chromosomes. *F The take home message from this work: Lethal P lines are likely to *F be lethal due to the insertion if they are still lethal after *F outcrossing for a number of generations. Excision work is relatively *F tedious and outcrossing itself does the job. *F >From rd120@gen.cam.ac.uk Thu Mar 02 16:18:43 2000 *F >To: abeaton@uclink4.berkeley.edu *F >Subject: Re: your pc *F Background lethals and l(2)k07736: *F You wrote: *F >The following strains were found to bear viable insertions and should *F >be changed to no P phenotype. *F .. *F >l(2)k07736 23F5-6 homozygotes *F .. *F so I am inclined to separate the lethality from the insertion i.e. *F P{lacW}Phas1k07736 will stay P{lacW}Phas1k07736 but will become *F marked as a viable insertion - presumbly the chromosome is still lethal *F so I will invent l(2)k07736k07736 with no associated P insertion, But *F (AND THIS IS THE PROBLEM) there are two other alleles of Phas1 that are *F also lethal, k07730 and k07739, both of these are said to fail to *F complement k07736. Is it likely that 3 inserts in Phas1 are all viable *F and all have a second hit in 'l(2)k07736'? Are these possibly a *F cluster with the same background lethal? The numbers are very close *F together, for what it is worth. *F From abeaton@uclink4.berkeley.edu Fri Mar 03 18:38:05 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: your pc *F that's exactly right. In l(2)k***** names the three digits following *F the k indicate the 'pool' number ie the bottle number of the cross *F and these need to be treated as a potential premeiotic cluster. The *F final two digits just count which one from the bottle. ... So *F yes all three k077* are a cluster with a shared background lethal. *F that's it, *F Amy # *U FBrf0126833 *a Dickson *b B. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:10:10 2000 *F To: dickson@nt.imp.univie.ac.at *F Subject: Helping FlyBase: ADRC-699B *F Dear Barry, *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Trio, Dock and Pak act in a common pathway controlling photoreceptor *F axon guidance.' *F You mention a gene that is new to FlyBase, Mtl. Does Mtl have a full *F name? Do you have a map location for Mtl? This is a data class we *F like to track. *F I am sending this to you because the first author of the abstract is *F not in FlyBase, but do feel free to pass it on to whoever is most *F approppriate. *F Thanks again for your help, *F with best wishes, *F Rachel. *F From dickson@nt.imp.univie.ac.at Fri Mar 03 13:37:46 2000 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 3 Mar 2000 13:37:46 +0000 *F Mime-Version: 1.0 *F Date: Fri, 3 Mar 2000 14:44:20 +0100 *F To: Rachel Drysdale (Genetics) *F From: Barry Dickson *F Subject: Re: Helping FlyBase: ADRC-699B *F Dear Rachel, *F Mtl is 'mig-2-like', and it maps to 98A. *F . *F . *F Thanks for your help, and your great work. *F Best wishes, *F Barry *F \-------------------------------------------------- *F Barry Dickson *F Research Institute of Molecular Pathology (I.M.P.) *F Dr. Bohr-Gasse 7 *F A-1030 Vienna *F Austria *F Tel. +43 1 797 30 421 *F Fax. +43 1 798 71 53 *F Email. dickson@nt.imp.univie.ac.at *F \-------------------------------------------------- # *U FBrf0126834 *a Simonelig *b M. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:47:43 2000 *F To: Martine.Simonelig@igh.cnrs.fr *F Subject: Helping FlyBase: ADRC-318B *F Dear Martine, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Functional analysis of the Drosophila Poly(A) *F polymerase gene required for mRNA 3'-end processing.' *F You mention a gene that is new to FlyBase, Pap. Do you have a map *F location for Pap? This is a data class we like to track. Is the P *F element insertion you mention a BDGP one? If so, please could you tell *F us the insertion identifier as this, too is a data class we like to *F keep track of. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Martine.Simonelig@igh.cnrs.fr Fri Mar 03 13:12:21 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-318B *F Dear Rachel, *F The gene I am describing in the abstract, that we called Pap, encodes *F Drosophila poly(A) polymerase. It is located in the P1 phage DS 06300. The *F P element inserted in the gene is l(2)k07618. I submitted the sequence of *F a Pap cDNA to Genbank recently. Accession number is: AF231704. *F . *F . *F I hope this is of any help. *F Best regards. *F Martine *F \----------------------------------------------------- *F Dr Martine SIMONELIG *F Genetique du Developpement de la Drosophile *F Institut de Genetique Humaine *F 141, rue de la Cardonille *F 34396 MONTPELLIER CEDEX 5. France. *F Tel: 33 4 99 61 99 59 *F Fax: 33 4 99 61 99 57 *F \----------------------------------------------------- # *U FBrf0126835 *a Dearolf *b C. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:46:17 2000 *F To: cdearolf@partners.org *F Subject: Helping FlyBase: ADRC-279B *F Dear Chuck, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Preferential transcriptional activation of *F Drosophila eukaryotic initiation factor 1A by hop Jak kinase *F in larval hemocytes.' *F And say 'We previously isolated and sequenced the gene for *F Drosophila translation initiation factor D-eIF1A (formerly D-eIF4c)'. *F The FlyBase record for this gene is below, and you abstract suggests *F that we should rename the gene to be 'eIF-1A'. We should also *F presumably change the \*Y lines to read: *F \*Y eukaryotic initiation factor 1A *F If you could confirm this for me I would be grateful. *F \*a eIF-4c *F \*z FBgn0026250 *F \*d cytosolic protein synthesis initiation ; GO:0006440 *F \*F cytosolic translation initiation factor ; GO:0003744 *F \*Y eukaryotic initiation factor 4C *F \*x FBrf0107670 == Dearolf, 1999.3.5, personal communication *F \*x FBrf0106997 == Myrick and Dearolf, 1999, A. Dros. Res. Conf. 40: 487C *F \*E FBrf0106997 == Myrick and Dearolf, 1999, A. Dros. Res. Conf. 40: 487C *F \*e Eukaryotic initiation factor 4c *F \*i D-eIF4c *F \*d cytosolic protein biosynthesis ; GO:0006416 *F \*F cytosolic translation initiation factor ; GO:0003744 *F \*Y eukaryotic initiation factor 4C *F \*E FBrf0107670 == Dearolf, 1999.3.5, personal communication *F \*c 90E--91A *F \*D Cytological location determined by hybridization to cytologically mapped *F \*D P1 clones. *F In looking into this I found another gene record that has 'eukaryotic *F initiation factor 1A' properties and wonder whether you have anything *F to say on whether this record could represent the same gene as eIF-1A *F (sadly no cytology in the FBgn0025959 record). *F \*a anon-EST:Liang-2.3 *F \*z FBgn0025959 *F \*g AI124295 *F \*d cytosolic protein synthesis initiation ; GO:0006440 | inferred from sequence similarity *F \*F cytosolic translation initiation factor ; GO:0003744 | inferred from sequence similarity *F \*Y eukaryotic initiation factor 1A-like *F \*j species == Homo sapiens; gene == EIF1A; GDB:126358; OMIM:603374 *F \*j species == Saccharomyces cerevisiae; gene == TIF11; SGDID:L0002306 *F \*j species == Triticum aestivum; gene == 'eIF-1A, translation initiation factor 1A'; SWP:P47815 *F \*x FBrf0106226 == Biggin, 1999.2.5, personal communication *F \*x FBrf0105875 == Liang and Biggin, 1998, Development 125(22): 4471--4482 *F \*E FBrf0106226 == Biggin, 1999.2.5, personal communication *F \*j species == Homo sapiens; gene == EIF1A; GDB:126358; OMIM:603374 *F \*j species == Saccharomyces cerevisiae; gene == TIF11; SGDID:L0002306 *F \*j species == Triticum aestivum; gene == 'eIF-1A, translation initiation factor 1A'; SWP:P47815 *F \*E FBrf0105875 == Liang and Biggin, 1998, Development 125(22): 4471--4482 *F \*i clone 2.3 *F \*d cytosolic protein biosynthesis ; GO:0006416 | inferred from sequence similarity *F \*F cytosolic translation initiation factor ; GO:0003744 | inferred from sequence similarity *F \*Y eukaryotic initiation factor 1A-like *F Thank you for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From CDEAROLF@partners.org Fri Mar 03 14:07:15 2000 *F To: 'Rachel Drysdale' *F Subject: RE: Helping FlyBase: ADRC-347A *F requests, *F 1) StatF is indeed stat92Efrankenstein *F 2) Yes, eIF-1A is the correct nomenclature, and it is the same gene as that *F identified in the paper from Mark Biggin's lab. My post-doc originally *F named it eIF-4C (its original name in the mammalian literature, before being *F changed to mammalian eIF-1A), but we then changed our nomenclature to *F conform with the current name. We have a paper (Myrick and Dearolf) that *F just appeared in Gene. *F Regards, *F Chuck Dearolf *F Charles Dearolf, Ph.D. *F Associate Professor *F Massachusetts General Hospital *F Jackson 1402, 55 Fruit St. *F Boston, MA 02114 *F Tel: 617-724-3165 *F Fax: 617-724-3248 *F Email: cdearolf@partners.org # *U FBrf0126836 *a Treisman *b J. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:42:01 2000 *F To: treisman@saturn.med.nyu.edu *F Subject: Helping FlyBase: ADRC-65 *F Dear Jessica, *F We are curating the abstracts for the upcoming Bellevue (Seattle) *F Annual Drosophila Research Conference, for FlyBase. I am writing (this *F time) in connection with your abstract: *F 'Actin-mediated changes in cell shape coordinate the timing of *F Drosophila photoreceptor differentiation.' *F You mention a gene that is new to FlyBase, acu. Do you have a map *F location for acu? This is a data class we like to track. *F Another abstract, number 441B: *F Act-up regulates actin organisation and cell polarity in *F Drosophila. B. Baum 1, Z. Wills 2, D. Van *F Vactor 2, N. Perrimon 1. 1) Dept Genetics, HHMI, *F Harvard Medical Sch, Boston, MA; 2) Dept Cell Biology, Harvard Medical Sch, *F Boston, MA. *F also mentions acu, is this the same acu as yours? *F Thank you for your help, *F with best wishes, *F Rachel. *F From rd120@gen.cam.ac.uk Fri Mar 03 10:03:11 2000 *F To: treisman@saturn.med.nyu.edu *F Subject: Helping FlyBase: ADRC-600B *F Dear Jessica, *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'blind spot is required for progression of differentiation across *F the Drosophila eye disc.' *F You mention a gene that is new to FlyBase, blind spot. Do you have a *F map location for blind spot? This is a data class we like to track. *F Unfortunately bls (the symbol you would like to use for blind spot) is *F already in use (bls: bractless, FBgn0028643). The symbol 'bli' is *F however available - would you like to use that for blind spot *F instead? *F Thank you for your help, *F with best wishes, *F Rachel. *F From treisman@saturn.med.nyu.edu Fri Mar 03 14:07:38 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-65 *F Dear Rachel, *F The map position of acu is 21F1-2 - it is the same gene studied by Norbert *F Perrimon's lab, and encodes a homologue of yeast cyclase associated *F protein. *F The map position of blind spot is 78A2, and I can switch to the *F abbreviation bli. *F Best wishes, *F Jessica Treisman *F Jessica Treisman *F Skirball Institute for Biomolecular Medicine *F Developmental Genetics Program *F NYU Medical Center *F 540 First Avenue *F New York, NY 10016 *F (212) 263-1031 *F (212) 263-7760 (FAX) # *U FBrf0126837 *a Odenwald *b W. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:12:05 2000 *F To: ward@codon.nih.gov *F Subject: Helping FlyBase: ADRC-730C *F Dear Ward, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'nerfin-1, a Zn-Finger Gene Dynamically Expressed *F During Neuroblast Lineage Development.' *F You mention a gene that is new to FlyBase, nerfin-2. *F Do you have a map location for nerfin-2? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From ward@codon.nih.gov Fri Mar 03 14:38:10 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: nerfin-2 map location *F Hi Rachel, *F nerfin-2 resides at 85F. *F Best Regards, *F Ward *F Ward F. Odenwald *F Neurogenetics Unit *F Laboratory of Neurochemistry *F Bld 36, Rm 4D-20, NINDS, NIH *F 36 Convent Dr MSC 4160 Phone 301-496-5940 *F Bethesda, Maryland 20892-4160 FAX 301-496-1339 # *U FBrf0126838 *a Theurkauf *b W. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:02:27 2000 *F To: William.Theurkauf@umassmed.edu *F Subject: Helping FlyBase: ADRC-536A *F Dear Bill, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'armitage: a novel axial patterning mutant affecting microtubule *F organization during Drosophila oogenesis.' *F You mention a gene that is new to FlyBase, armitage. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. Also, have you an abbreviation, or shall we *F just use 'armitage' as the symbol for now? *F Thank you for your help, *F with best wishes, *F Rachel. *F From william.theurkauf@umassmed.edu Fri Mar 03 14:43:51 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-536A *F Dear Rachel, *F The armitage mutation is located at 63D2 immediately adjacent to *F cyclin J. The abbreviation we are using is armi. *F Best wishes, *F Bill Theurkauf *F Program in Molecular Medicine *F University of Massachusetts Medical Center *F 373 Plantation Street *F Worcester, MA 01605 *F Phone: 508-856-4900 *F Fax: 508-856-4289 *F e-mail: william.theurkauf@ummed.edu # *U FBrf0126839 *a Edwards *b K. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:44:19 2000 *F To: kaedwar@ilstu.edu *F Subject: Helping FlyBase: ADRC-186B *F Dear Kevin, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Complex distribution of breakpoints following P element imprecise *F excision in the presence of a homologue.' *F You mention a gene that is new to FlyBase, Gef26. *F Do you have a map location for Gef26? This is a data class we like to track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kaedwar@ilstu.edu Fri Mar 03 14:47:07 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-186B *F Gef26 is at 26C. This comes from the known localization of l(2)k13720 *F http://flybase.bio.indiana.edu:82/.bin/fbidq.html?FBgn0021873, *F which is an allele of Gef26 by molecular and phenotypic criteria. *F I independently confirmed that l(2)k13720 in situs to 26C. *F Kevin Edwards *F \-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= *F Dr. Kevin A. Edwards *F Dept. of Biological Sciences *F Illinois State University *F Box 4120 *F Normal, IL 61790-4120 *F Office 309-438-7689 *F Fax 309-438-3722 *F Lab 309-438-5075 *F http://www.bio.ilstu.edu/Edwards/webproj/labhome.htm *F kaedwar@ilstu.edu *F \-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= # *U FBrf0126840 *a Seher *b T. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:39:17 2000 *F To: mleptin@uni-koeln.de *F Subject: Helping FlyBase: ADRC-24 *F Hi Maria, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The trouble without tribbles : Mitosis *F interfering with mesodermal morphogenesis.' *F You mention a gene that is new to FlyBase, tribbles. Is there an *F abbreviation that you are using for this gene? *F Do you have a map location for your tribbles? This is a data class we *F like to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From T.Seher@uni-koeln.de Fri Mar 03 14:53:39 2000 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Fwd: Helping FlyBase: ADRC-24 *F Dear Rachel Drysdale, *F the abbreviation for tribbles is trbl . It maps to 77C according to the *F phenotype (we look at) found in the two deficiencies rdgCco2 and ri79c. *F There are also two P-elements showing the phenotype which have been mapped *F to this region by in situ hybridization. *F I hope this helps, *F best regards, *F Thomas Seher. *F Thomas SEHER *F Universitaet Koeln *F Institut f. Genetik *F Abt. Prof. M. LEPTIN *F Weyertal 121 *F 50931 KOELN *F GERMANY *F phone number : 0049-221-470-3414 *F Fax : 0049-221-470-5264 *F email : T.Seher@Uni-Koeln.DE # *U FBrf0126841 *a Lehmann *b R. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:48:11 2000 *F To: lehmann@saturn.med.nyu.edu *F Subject: Helping FlyBase: ADRC-338A *F Dear Ruth, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterizing a gene involved in gonadogenesis.' *F You mention a gene that is new to FlyBase, but do not name it. Does it *F correspond to one of the CK ESTs? *F Do you have a map location for your gene? This is a data class we like *F to track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From lehmann@saturn.med.nyu.edu Fri Mar 03 15:17:36 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-338A and 85 *F Hi, there are a number of abstract from my lab, so I am not quite sure *F which one this is. *F One gene that should be entered into flybase is wunen-2 *F . *F . *F . *F The other gene may be in a report by Lilach Gilboa, is a transcript *F specifically expressed in gonadal mesoderm during all stage of development. *F This gene was identified from a Kopczynski/ Goodman CDNA library (Proc Natl *F Acad Sci U S A. 1998 Aug 18;95(17):9973-8.). The clone is called ck00048. *F The gene has no homology to any other gene in the fly or nay other data *F base. We do not have mutants yet. *F If you need more info, we are glad to provide it to you, best regards ruth *F lehmann. *F Dr. Ruth Lehmann *F HHMI and Skirball Institute *F Developmental Genetics Program (4th floor) *F New York University Medical School *F 540 First Ave *F New York, NY 10016 *F phone 212-263 8071 *F Fax 212-263 7760 *F Email lehmann@saturn.med.nyu.edu # *U FBrf0126842 *a Montell *b D. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:41:15 2000 *F To: dmontell@jhmi.edu *F Subject: Helping FlyBase: ADRC-52 *F Dear Denise, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'taiman (tai), a gene encoding a steroid receptor coactivator-like *F protein, is required for border cell migration in Drosophila ovaries.' *F You mention a gene that is new to FlyBase, tai. Do you have a map *F location for your gene? This is a data class we like to track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From dmontell@jhmi.edu Fri Mar 03 15:29:02 2000 *F Subject: Fwd: Helping FlyBase: ADRC-52 *F To: rd120@gen.cam.ac.uk *F The location is 30A. # *U FBrf0126843 *a Bejsovec *b A. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:02:32 2000 *F To: bejsovec@nwu.edu *F Subject: Helping FlyBase: ADRC-560A *F Dear Amy, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of blimp and zeppelin, two *F genes required for normal epidermal development.' *F You mention a gene that is new to FlyBase, zeppelin. Except that we *F are not completely sure that it is new, and I need to make sure. We have *F zep: zeppelin as a synonym for psq: pipsqueak. Since that particular *F zeppelin dates back to 1996 (FBrf0088214 == Horowitz and Berg, 1996, *F Development 122(6): 1859--1871) I sincerely doubt that it is the same *F as your zeppelin, but I have to check. *F Also, do you have a map location for zeppelin? You know what we are *F like about our map data .... *F Thank you for your help, *F Rachel. *F From bejsovec@lulu.acns.nwu.edu Fri Mar 03 15:58:21 2000 *F To: Rachel Drysdale (Genetics) *F From: bejsovec@lulu.acns.nwu.edu (Amy Bejsovec) *F Hi Sweetie, *F Our zeppelin is completely unrelated (unless there is some *F extremely weird cosmic coincidence). It maps to the centromeric *F heterochromatin on the third. blimp and zeppelin are both named for the *F extreme ballooning of the embryos when we squash them under coverslips in *F the course of doing cuticle preparations. They seem to result in some sort *F of stretchy-skin or -cuticle effect. *F \-- Amy # *U FBrf0126844 *a Montell *b D. *t 2000.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:08:25 2000 *F To: dmontell@jhmi.edu *F Subject: Helping FlyBase: ADRC-670C *F Dear Denise, *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of Rho family GTPases in Drosophila.' *F You mention a gene that is new to FlyBase, RhoX. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F You mention two human homologues of RhoX. Would you be able to tell us *F which human genes these are? Again, this is a very useful class of *F data to have in FlyBase. *F Thank you for your help, *F with best wishes, *F Rachel. *F From dmontell@jhmi.edu Fri Mar 03 16:26:34 2000 *F Subject: Re: Helping FlyBase: ADRC-670C *F To: Rachel Drysdale *F The cytology is 77B6-9 as determined by Todd Laverty. The human homologs are KIAA0740 and KIAA0717 proteins which were identified based on sequencing of cDNAs from a random sequencing project. # *U FBrf0126845 *a Bourbon *b H.M. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:59:28 2000 *F To: cribbs@cict.fr *F Subject: Helping FlyBase: ADRC-512A *F Dear David, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Developmental roles of the mediator complex.' *F You mention a gene that is new to FlyBase, Trap80. *F Do you have a map location for Trap80? This is a data class we like to *F track. You say 'Via the Genome Project we identified a second *F Drosophila TRAP subunit homologue, dTRAP80, and examined P *F insertion-derived mutations of this gene' which suggests to us that a *F genome project insertion mutant falls in Trap80. Could you tell us the *F insertion identifier for the line(s) in question? Also if Trap80 *F corresponds to any ESTs that is also very useful information for us to *F have. These correspondences are all important for bringing together *F the various data-bits about a gene into one gene record. *F Thank you for your help, *F with best wishes, *F Rachel. *F From bourbon@cict.fr Fri Mar 03 16:46:31 2000 *F To: rd120@gen.cam.ac.uk *F Subject: TRAP80 *F Dear Rachel, *F I am writing to you on behalf of David Cribbs about the abstract (#512A) *F we submitted to the 41th ADRC. TRAP80 is located at 90F1-2. The insertion *F identifier for this gene is l(3)s2956. The identifiers for two available *F ests are CK00124 and SD10038. *F I hope these informations will be sufficient to anotate TRAP80 on FlyBase. *F Best regards *F Henri-Marc Bourbon, phD *F Dr. Henri-Marc Bourbon, phD *F Centre de Biologie du Développement *F UMR5547 du C.N.R.S. *F Université Paul Sabatier Toulouse III *F 118 Route de Narbonne *F 31062 Toulouse *F France *F e-mail: bourbon@cict.fr *F phone: (33) 05 61 55 82 88 *F Fax: (33) 05 61 55 65 05 # *U FBrf0126846 *a Duronio *b R. *t 2000.2.2 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:54:29 2000 *F To: duronio@med.unc.edu *F Subject: Helping FlyBase: ADRC-429B *F Dear Bob, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila stem loop binding protein (dSLBP) coordinates accumulation of histone mRNA with cell cycle progression. ' *F You mention a gene that is new to FlyBase, Slbp. Do you have a map location for your gene? This is a data class we track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From duronio@med.unc.edu Fri Mar 03 17:17:38 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-429B *F Hi Rachel, *F Absolutely, I have a map position for you. Slbp is at 98F on 3R. It's on *F cosmid 1H12 from EDGP. *F Let me know if you need anything else. *F Best wishes, *F Bob *F Bob Duronio *F Department of Biology *F Box 3280 *F University of North Carolina *F Chapel Hill, NC 27599-3280 *F Phone: (919) 962-7749 *F FAX: (919) 962-8472 *F email: duronio@med.unc.edu # *U FBrf0126847 *a McGinnis *b W. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:40:16 2000 *F To: mcginnis@biomail.ucsd.edu *F Subject: Helping FlyBase: ADRC-38 *F Dear Bill, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A role for the Drosophila tubby-like protein (Dtulp) in *F olfactory sensory transduction.' *F You mention a gene that is new to FlyBase, tulp. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F We already have a record for a gene that is a homolog of the mouse *F tubby gene, do you think it is the same gene as yours or is it *F something different? *F \*a king-tubby *F \*z FBgn0015721 *F \*e king tubby *F \*i ESTS:87D3S *F \*c 57B--C *F \*c Left limit from in situ hybridisation (FBrf0071734) *F \*c Right limit from in situ hybridisation (FBrf0071734) *F \*g Z50688; dbSTS:24647 *F \*j species == Homo sapiens; gene == TUB; GDB:4204602; OMIM:601197 *F \*j species == Mus; gene == tub; MGI:98868 *F \*r This gene was first recognised by a match of an *F \*r STS sequence from a European Drosophila Genome Mapping Project cosmid. *F \*x FBrf0071734 == EDGP Project Members, 1994-, computer file *F \*x FBrf0106792 == Kuhnlein and Jackle, 1999, A. Dros. Res. Conf. 40: 558B *F \*x FBrf0098301 == Louis et al., 1997, Gene 195(2): 187--193 *F \*E FBrf0106792 == Kuhnlein and Jackle, 1999, A. Dros. Res. Conf. 40: 558B *F \*E FBrf0071734 == EDGP Project Members, 1994-, computer file *F \*i ESTS:87D3S *F \*c 57B--C (determined by in situ hybridisation) *F \*j species == Homo sapiens; gene == TUB; GDB:4204602; OMIM:601197 *F \*j species == Mus; gene == tub; MGI:98868 *F \*E FBrf0098301 == Louis et al., 1997, Gene 195(2): 187--193 *F \*i ESTS:87D3S *F \*c 57B--C (determined by in situ hybridisation) *F Thank you for your help, *F with best wishes, *F Rachel. *F From mcginnis@ucsd.edu Fri Mar 03 17:18:50 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-38 *F Hi Rachel, *F Yes, our D-tulp is the same as what Jackle has been calling king *F tubby. We used D-tulp because it conforms to the mammalian *F nomenclature, which was in place years before the fly gene cloning *F and characterization. In mammals, tubby was the founding gene, and *F the additional paralogs (3 in number) that exist in mice and humans *F have been designated tulp1, tulp2, and tulp3, because their genetic *F function is unknown. Tulp stands for genes that encode tubby-like *F proteins. *F The cytogenetic location of the gene is 57C1, and the 3' end of the *F D-tulp transcription unit is approximately 3.5 kb from the 5' end of *F the mago nashi transcription unit. D-tulp maps more proximal on 2R *F than does mago nashi. *F . *F . *F Bill *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F William McGinnis Phone 858-822-0458 *F Department of Biology 0349 FAX 858-822-0460 *F University of California, San Diego email: mcginnis@biomail.ucsd.edu *F 9500 Gilman Drive http://www.biology.ucsd.edu/labs/mcginnis *F La Jolla, CA 92093-0349 *F NOTE NEW AREA CODE FOR UCSD, 858- versus 619- *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ # *U FBrf0126848 *a Stevaux *b O. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:52:27 2000 *F To: stevaux@helix.mgh.harvard.edu *F Subject: Helping FlyBase: ADRC-399B *F Dear Olivier, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of RBF2, a novel pocket protein in Drosophila *F melanogaster.' *F You mention that Rbf2 is represented by two ESTs. Are these BDGP *F ESTs? If so please could you tell me which ones - we track this class *F of data. You also mention three P-elements. Are these BDGP/Szeged *F insertions? If so could you let me know their identifiers - we track *F this class of data too. *F Thank you for your help, *F with best wishes, *F Rachel. *F From stevaux@helix.mgh.harvard.edu Fri Mar 03 17:34:35 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-399B *F Dear Rachel, *F The three P-elements are thew following ones: P-1732 or l(3)08724 (BDGP), *F P-518 or l(3)S028404 (Szeged) and P-2000 or l(3)125011 (Szeged). P-2000 is *F actually not a lethal, but a male sterile at 25-C and perfectly alive and *F fertile at 18-C. They all map 5' of Moira in the 89B1 region. *F The two ESTs I refer to are from the BDGP and they are LD 02737 and LD *F 15806. They are both full lenght clones and LD 02737 has the exact same *F sequence than the genomic sequence released by Celera. *F Best regards, *F Olivier # *U FBrf0126849 *a Hashimoto *b C. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:00:18 2000 *F To: carl.hashimoto@yale.edu *F Subject: Helping FlyBase: ADRC-527A *F Dear Dr. Hashimoto, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of a serine protease inhibitor in the serpin family from *F Drosophila.' *F You mention a gene that is relatively new to FlyBase, sp6. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Carl.Hashimoto@yale.edu Fri Mar 03 18:24:07 2000 *F Subject: Re: Helping FlyBase: ADRC-527A *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F We did not map the gene for sp6, but in the paper we report that an EST *F matching sp6 (GM04155) is contained within a P1 clone that roughly maps to *F 55A-B. I think we couldn't be more specific about the map location because *F of some ambiguity in the database about the endpoints of the P1 clone. *F The P1 clone is DS03544. Hope this information is helpful. *F Best wishes, *F Carl # *U FBrf0126850 *a Chase *b B. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:13:00 2000 *F To: Bruce_Chase@unomaha.edu *F Subject: Helping FlyBase: ADRC-747B *F Dear Dr. Chase, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Drosophila Gene Mind-Meld Encodes A Novel ADAM *F Protein Highly Expressed During CNS Development.' *F You mention a gene that is new to FlyBase, mind-meld. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. Also, do you have a short symbol in mind for *F this gene? *F We wonder whether your mind-meld is the same as the 'mm: mindmelt' gene *F (FBgn0015514) which is already in FlyBase, since there are similarities *F in the biology as well as in the name. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F From Bruce_Chase/CAS/UNO/UNEBR@unomail.unomaha.edu Fri Mar 03 18:24:58 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-747B *F Hi Rachel, *F The mind-meld gene is newly described in the abstract, and is yet not published. *F hasn't been taken) would be just fine as a symbol. We will be submitting the *F nearly full-length cDNA sequence we have to GENBANK very soon .... Bruce # *U FBrf0126851 *a Ahmad *b K. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:45:24 2000 *F To: kahmad@fred.fhcrc.org *F Subject: Helping FlyBase: ADRC-245A *F Dear Kami, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Centromeres coincide with the first set of heterochromatic replication origins.' *F You mention a gene that is new to FlyBase, Cid. *F Do you have a map location for your gene? This is a data class we like to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kahmad@fred.fhcrc.org Fri Mar 03 19:07:01 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-245A *F dear rachel - it is a pleasure to help *F flybase, especially on the matter of the *F the gene cid (for centromere identifier) *F was reported in Henikoff, S., K. Ahmad, *F J. S. Platero, and B. van Steensel (2000). *F Heterochromatic deposition of centromeric *F histone H3-like proteins. PNAS 97:716-721. *F the gene sequence is contained within a *F Berkeley Genome Project sequence, AC005652, *F and is at 50A on the cytological map, between *F the arr and drk genes (defined by sequence *F analysis and more recently by complementation *F with deficiencies in the region - this latter *F stuff is not yet published). is this the info *F that you need? -yours, kami # *U FBrf0126852 *a Stewart *b R. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 14:54:04 2000 *F To: rodney.stewart@yale.edu *F Subject: Helping FlyBase: ADRC-683A *F Dear Rodney, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of the lats tumor suppressor signaling pathway: *F lats gets flik-ed.' *F You mention a gene that is new to FlyBase, flik. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rodney.stewart@yale.edu Fri Mar 03 19:10:51 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-683A *F Dear Rachel, the flik mutation maps to cytological position 86E and *F is uncovered by the Df(3R)M-Kx1 deletion. I hope this information is *F useful. Regards, *F Rod Stewart. # *U FBrf0126853 *a Pallanck *b L. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:13:19 2000 *F To: pallanck@genetics.washington.edu *F Subject: Helping FlyBase: ADRC-756B *F Dear Leo, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of a putative Drosophilahomolog of the gene responsible *F for Spinocerebellar Ataxia Type 2.' *F You mention a gene that is new to FlyBase, Sca2. Do you have a map location for your gene? This is a data class we like to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From pallanck@genetics.washington.edu Fri Mar 03 19:13:29 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-756B *F Hi Rachel, *F The Drosophila SCA2 gene maps by in situ hybridization to 88F-89A1. *F This localization is confirmed by in situ hybridization carried out by the *F BDGP using BAC probes that contain the SCA2 gene. We also have an abstract *F describing the Drosophila parkin gene. We haven't mapped the Parkin *F gene, but it is represented by bac clones that hybridize to 78B2-78D2. *F Best regards, *F \-Leo *F Leo Pallanck *F Department of Genetics, Box 357360 *F University of Washington *F Seattle, WA 98195 *F office ph: (206) 616-5997 *F lab ph: (206) 616-8570 *F FAX: (206) 543-0754 # *U FBrf0126854 *a Rennebeck *b G. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:46:32 2000 *F To: grenne2@pop.uky.edu *F Subject: Helping FlyBase: ADRC-290A *F Dear Gaby, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'dSOCS as a negative regulator of the JAK/STAT signaling *F cascade in flies.' *F You mention a gene that is new to FlyBase, Socs. *F Do you have a map location for Socs? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From grenne2@pop.uky.edu Fri Mar 03 19:18:17 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-290A *F Here is some information about dSOCS. *F It maps to 36E1-36E4. *F A P1 clone called DS04489 contains dSOCS gene, however the limits of the cDNAs *F Please let me know if you need more information. *F Thank you for your email. *F Til later, *F Gaby # *U FBrf0126855 *a Gates *b J. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:02:51 2000 *F To: jgates@howard.genetics.utah.edu *F Subject: Helping FlyBase: ADRC-569A *F Dear Julie, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A screen for ecdysone-regulated genes required for the *F morphogenesis of adult appendages during metamorphosis.' *F You mention two BDGP P insertion mutations - one identifying an hnRNP K *F homologue and the other a putative septate junction component. Please *F could you tell me the identifiers for these insertions? - we track this *F class of data. FlyBase already has a gene record for HnRNP-K. Is this *F the same hnRNP K gene that you have identified or is yours a second, *F distinct one (or perhaps you do not yet know)? *F Thank you for your help, *F with best wishes, *F Rachel. *F From jgates@howard.genetics.utah.edu Fri Mar 03 20:17:47 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-569A *F Rachel, *F l(2)k08305=hnRNP K, bancal *F l(2)07022=putative septate junction component, vulcan *F I will be submitting a paper describing the screen I did within the next *F month or so. The hnRNP K has already been cloned by Steven Kerridge's *F group and they called it bancal. I have decided to call the putative *F septate junction component vulcan. If you need any further information *F please let me know. *F Julie # *U FBrf0126856 *a Schupbach *b T. *t 2000.3.3 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:08:48 2000 *F To: gschupbach@molbiol.princeton.edu *F Subject: Helping FlyBase: ADRC-676C *F Dear Dr. Schupbach, *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification and characterization of gritz, a *F novel putative ligand for the Drosophila Egf receptor.' *F You mention a gene that is new to FlyBase, gritz. Do you have a map *F location for gritz? This is a data class we like to track, if *F possible. *F I am writing to you because the first author does not have an address *F in FlyBase - please feel free to pass my query on if there is someone better *F placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From gschupbach@molbio.princeton.edu Fri Mar 03 22:47:32 2000 *F To: 'Rachel Drysdale' *F Subject: RE: Helping FlyBase: ADRC-676C *F Dear Rachel, *F We localized gritz by polytene in situ hybridization to 74EF, it is deleted *F by the Deficiency Df(3L)81k19. *F We don't have a mutant phenotype yet, so have not localized it by *F traditional genetic mapping. *F I hope this helps. *F Yours, *F Trudi Schupbach # *U FBrf0126857 *a Schupbach *b T. *t 2000.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:50:17 2000 *F To: gschupbach@molbiol.princeton.edu *F Subject: Helping FlyBase: ADRC-366B *F Dear Dr. Schupbach, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Targets of dpp and Egfr signalling during *F oogenesis.' *F You mention a gene that is new to FlyBase, *F CTP:phosphocholine-cytidylyltransferase. Do you have a map location *F for your gene? This is a data class we like to track, if possible. *F Do you have a short symbol by which you refer to it? *F I am writing to you because the first author does not have an address *F in FlyBase - please feel free to pass my query on if there is someone better *F placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From gschupbach@molbio.princeton.edu Fri Mar 03 22:55:45 2000 *F To: 'Rachel Drysdale' *F Subject: RE: Helping FlyBase: ADRC-366B *F Dear Rachel, *F this gene is contained in a P1 clone called DS05969, that lies proximal to *F DS02734 which has been mapped to 62A1-2, so the gene should be in 62A. It is *F deleted in the Df(3L)Ar14-8. *F . *F . *F Yours, *F Trudi Schupbach. # *U FBrf0126858 *a Prokopenko *b S. *t 2000.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:11:11 2000 *F To: sp691305@imgen.bcm.tmc.edu *F Subject: Helping FlyBase: ADRC-727C *F Dear Sergei, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'From phenotype to gene function: A molecular screen for novel genes *F required for the development of the peripheral nervous system.' *F Your abstract suggests that Calreticulin and pot pourri are allelic, *F but since we have somewhat different map locations for these geens in *F FlyBase (potp: 85E and Crc:86B--86C) I wanted to be sure that is what *F you meant before I merge the gene records. *F Thank you for your help, *F with best wishes, *F Rachel. *F From sp691305@imgen.bcm.tmc.edu Sat Mar 04 19:54:50 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-727C *F Dear Rachel, *F > Your abstract suggests that Calreticulin and pot pourri are allelic, *F > but since we have somewhat different map locations for these geens in *F > FlyBase (potp: 85E and Crc:86B--86C) I wanted to be sure that is what *F > you meant before I merge the gene records. *F Yes, this is exactly what I meant. I have isolated plasmid rescues from *F the 1143/7 potp P element and found that their sequence is identical to *F the published mRNA sequence of calreticulin. Plasmid rescues where mapped *F to the same cytological location as the P insertion (85E). *F In addition, we mapped the LD07621 calreticulin cDNA (BDGP) to 85E1. *F Therefore, I concluded that the published mapping position for *F calreticulin (86B-C) is incorrect. *F Please, let me know if you have any further question. If it can be of help *F for you, I would be glad to send you a copy of the manuscript on molecular *F screen when we submit it to Genetics by the end of March. *F Best wishes, *F Sergei *F Sergei N. Prokopenko *F Program in Developmental Biology *F Baylor College of Medicine *F Rm. T634, Mail stop BCM235 *F One Baylor Plaza *F Houston, TX 77030, USA *F (713) 798-5273 *F (713) 798-8515 fax *F sp691305@bcm.tmc.edu *F ================================ # *U FBrf0126859 *a Eldon *b E. *t 2000.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:04:33 2000 *F To: eldon.1@nd.edu *F Subject: Helping FlyBase: ADRC-623A *F Dear Elizabeth, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Testing the usefulness of attacin reporter *F constructs as monitors of 18-wheeler activity in the humoral immune *F response.' *F You mention a gene that is new to FlyBase, AttB. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Another abstract, 627B (Variation in Immune Response in Drosophila *F melanogaster at the Molecular and Phenotypic Levels, by Lazzaro and *F Clark) describes an attacin B. Do you know whether this is the same *F gene as your AttB? If they are the same gene they should be *F represented by one gene record in FlyBase, but if they are not they *F should be represented by two records - which is why I ask. *F Thank you for your help, *F with best wishes, *F Rachel. *F From elizabeth.d.eldon.1@nd.edu Sun Mar 05 01:19:43 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-623A *F Rachel, *F ...The attacin B gene to which our abstract refers is closely linked (ca. 7 kb *F downstream) to the attacin A gene, and its sequence has been submitted to *F genbank. The 5' regulatory sequence is AF220546, and the transcription unit *F (including intron) is AF220547. *F . *F . *F . *F Sincerely, *F Beth *F Elizabeth D. Eldon, Ph.D. *F Department of Biological Sciences *F University of Notre Dame *F Notre Dame, IN 46556 *F (219) 631-5494 *F eldon.1@nd.edu *F (219) 631-7413 (fax) # *U FBrf0126860 *a Funakoshi *b Y. *t 2000.3.4 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:01:23 2000 *F To: yokofuna@ims.u-tokyo.ac.jp *F Subject: Helping FlyBase: ADRC-538C *F Dear Yoko, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'disc6 encodes a novel wing patterning gene which regulates *F expression of the major Dpp receptor, thick veins.' *F You mention a gene that is new to FlyBase, disc6. Do you have a map *F location for disc6, as map location is a data class that we are keen to track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From yokofuna@ims.u-tokyo.ac.jp Sun Mar 05 05:42:02 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-538C *F Dear Rachel, *F . *F . *F . *F By the way, we called the new gene as disc6 tentatively when I wrote *F abstruct for the meeting, but recently I named it as 'master of thickveins *F (mtv for abbrviation)' because this gene downregulates thckveins expression *F in wing discs. *F Thank you for your contribution to Flybase. Take care. *F n n YOKO FUNAKOSHI *F (^o^)/ yokofuna@ims.u-tokyo.ac.jp *F /( ) IMCB, Univ. of Tokyo *F & & telephone: 81-3-5841-7866 # *U FBrf0126861 *a Tang *b A. *t 2000.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:42:26 2000 *F To: amy_tang@uclink4.berkeley.edu *F Subject: Helping FlyBase: ADRC-69 *F Dear Amy, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A Novel Transcriptional Regulator, Lilliputian, is Required for Cell *F Size Control in Drosophila Development.' *F You mention a gene that is new to FlyBase, lilli. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From amy_tang@uclink4.berkeley.edu Sun Mar 05 07:08:49 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-69 *F Dear Rachel: *F The gene is located at *F 23C1-2. It has been cloned and sequenced. If you need further *F information about it, please do not hesitate to contact me. *F Thanks. *F Best regards, *F Amy *F ________________________________________ *F Amy Tang, Ph. D *F Gerald M. Rubin lab *F Room 539, Life Science Addition Building *F Dept. of Molecular and Cell Biology *F University of California *F Berkeley, CA 94720 *F Tel: (510) 643-9943 or (510) 643-9944 *F Fax: (510) 643-9947 *F E.mail: amy_tang@uclink4.berkeley.edu *F ________________________________________ # *U FBrf0126862 *a Kramer *b H. *t 2000.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:55:02 2000 *F To: kramer@utsw.swmed.edu *F Subject: Helping FlyBase: ADRC-432B *F Dear Helmut, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Classic Drosophila eye color mutations as new tools to study *F membrane trafficking.' *F You mention a gene that is new to FlyBase, Vps16. Do you have a map *F location for your gene? This is a data class we like to track, if *F possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kramer@utsw.swmed.edu Sun Mar 05 18:17:59 2000 *F Subject: Re: Helping FlyBase: ADRC-432B *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F The gene encoding the Drosophila homolog of he yeast protein Vps16p is *F located at 85D8 based on its inclusion in the the BAC clone CSC:AC014256 *F next to the Drosophila Tryptophanyl-tRNA synthetase, abbreviated *F 'Aats-trp'. *F We have not yet identified a mutation in this gene. *F sincerely *F Helmut # *U FBrf0126863 *a Bellen *b H. *t 2000.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:14:32 2000 *F To: hbellen@bcm.tmc.edu *F Subject: Helping FlyBase: ADRC-791A *F Hi Hugo, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'HRS regulates endosomal trafficking.' *F You mention a gene that is new to FlyBase, hrs. *F Do you have a map location for your gene? You know us and map data .... *F Thank you for your help, *F with best wishes, *F Rachel. *F From hbellen@bcm.tmc.edu Sun Mar 05 21:32:12 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-791A *F 23A, close to synaptotagmin *F Hugo J. Bellen *F Howard Hughes Medical Institute *F Charles Darwin Professor in Genetics *F Director, Program in Developmental Biology *F Baylor College of Medicine T630 *F Houston, TX 77030 *F Tel. 713-798-5272 *F Fax. 713-798-8515 *F email. hbellen@bcm.tmc.edu # *U FBrf0126864 *a Karim *b F. *t 2000.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 14:47:04 2000 *F To: felixk@exelixis.com *F Subject: Helping FlyBase: ADRC-397C *F Dear Felix, *F I do hope this gets to you at Exelixis - your email address is out of *F date in FlyBase and I made a guess at your Exelixias address *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila p53 Displays Structural and Functional *F Conservation with Human p53.' *F You mention a gene that is new to FlyBase, p53. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From felixk@exelixis.com Mon Mar 06 06:35:37 2000 *F Subject: Re: Helping FlyBase: ADRC-397C *F To: 'Rachel Drysdale' *F Rachel, *F The gene we are calling Dmp53 (The Drosophila homolog of p53) maps to *F 94D. ..... *F Felix *F \------------------------------ *F Felix Karim, Ph.D. *F Genetics Department *F Exelixis Inc. *F 260 Littlefield Ave *F So. San Francisco, CA 94080 *F phone: (650) 825-4247 *F fax: (650) 825-4240 *F felixk@exelixis.com # *U FBrf0126865 *a Schejter *b E. *t 2000.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:12:19 2000 *F To: lveyal@weizmann.weizmann.ac.il *F Subject: Helping FlyBase: ADRC-742C *F Dear Eyal, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila homologs of the mammalian WASP/SCAR protein *F family mediate various aspects of neurogenesis.' *F You mention a gene that is new to FlyBase, Scar. *F Do you have a map location for your gene or a symbol for your mutant *F allele? These are all data items that we track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Eyal.Schejter@weizmann.ac.il Mon Mar 06 12:48:28 2000 *F Subject: Re: Helping FlyBase: ADRC-742C *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F The Drosophila gene, which is a homolog of the evolutionarily conserved SCAR *F genes (both Dicty and human homologs have been characterized), maps to 32C. *F .... *F All the best, *F Eyal Schejter *F Dept. of Molecular Genetics *F Weizmann Institute of Science *F 76100 Rehovot ISRAEL *F Tel: 972-8-9342207 *F Fax: 972-8-9344108 *F e-mail: Eyal.Schejter@weizmann.ac.il # *U FBrf0126866 *a Silverman *b N. *t 2000.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 14:46:33 2000 *F To: nsilverm@fas.harvard.edu *F Subject: Helping FlyBase: ADRC-128 *F Dear Neal, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The role of a Drosophila IkB kinase in the insect immune response.' *F You mention a gene that is new to FlyBase, IKKgamma. *F Do you have a map location for IKKgamma? This is a data class we like *F to track. *F Thank you for your help, *F with best wishes, *F Rachel. *F From nsilverm@fas.harvard.edu Mon Mar 06 14:31:14 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-128 *F Hi Rachel, *F Thank you for including my abstract in flybase. Yes, I do know the map *F location for DmIKKgamma- 60E. ... *F Thanks *F Neal *F Neal Silverman *F Harvard University *F 7 Divinity Ave *F Cambridge MA 02138 *F nsilverm@fas.harvard.edu # *U FBrf0126867 *a Jones *b R. *t 2000.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:45:06 2000 *F To: rjones@mail.smu.edu *F Subject: Helping FlyBase: ADRC-235C *F Dear Richard, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'dSAP18 is an E(Z) interacting protein.' *F You mention a gene that is new to FlyBase, Sap18. *F Do you have a map location for Sap18? This is a data class we like *F to track, if possible. *F I am writing to you because the first author does not have an address *F in FlyBase - please feel free to pass my query on if there is someone better *F placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rjones@mail.smu.edu Mon Mar 06 22:08:41 2000 *F Subject: Re: Helping FlyBase: ADRC-235C *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F dSAP18, which is 58% identical to human SAP18, is located ~43 kb distal to *F Sb/sbd within the 89B9-10 region. Human SAP18 was originally identified as *F a component of SIN3 complexes. It was also described in an abstract from *F Steve Hanes' lab at the 1999 Ann. Dros. Conf. (Zhu and Hanes, Program No. *F 070). However, they referred to the protein as Bin1 (Bicoid Interacting *F Protein \#1). *F Best regards, *F Rick *F \------------------------------------- *F Richard S. Jones *F Dept. of Biological Sciences *F Fondren Science Building *F Southern Methodist University *F Dallas, TX 75275-0376 *F TEL: 214-768-3810 *F FAX: 214-768-3955 *F email: rjones@mail.smu.edu *F \------------------------------------- # *U FBrf0126868 *a Uemura *b T. *t 2000.2.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:06:11 2000 *F To: tuemura@take.biophys.kyoto-u.ac.jp *F Subject: Helping FlyBase: ADRC-643C *F Dear Tadashi, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A Novel MAP Kinase Phosphatase Controls Reorganization of Actin *F Cytoskeleton.' *F You mention a gene that is new to FlyBase, encoding a novel MKP. The *F thing is that we already have a gene called Mkp in FlyBase and I am *F wondering whether or not it corresponds to the Mkp you describe. Here *F is what we have for the Mkp that we already know about: *F \*a Mkp *F \*z FBgn0015764 *F \*F protein serine/threonine phosphatase ; GO:0004722 ; EC:3.1.3.16 *F \*Y MAP kinase-specific phosphatase *F \*j species == Homo sapiens; gene == DUSP2; GDB:139200 *F \*x FBrf0083847 == Cornelius and Engel, 1995, Cell. Signal. 7(6): 611--615 *F \*E FBrf0083847 == Cornelius and Engel, 1995, Cell. Signal. 7(6): 611--615 *F \*e MAP kinase-specific phosphatase *F \*i D-mkp *F \*F protein serine/threonine phosphatase ; GO:0004722 ; EC:3.1.3.16 *F \*Y MAP kinase-specific phosphatase *F \*j species == Homo sapiens; gene == DUSP2; GDB:139200 *F \*r A MAP kinase-specific phosphatase activity was found to be activated by *F \*r heat-shock to Schneider 2 cells. The gene was cloned using a @H.sapiens@ *F \*r PAC1 probe. *F If this isn't your gene then we need to settle on a distinguishing *F symbol and if you could tell me a map location than that would help *F keep the records straight. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tuemura@take.biophys.kyoto-u.ac.jp Tue Mar 07 08:12:47 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-643C *F Dear Rachel, *F Thank you for your email of March 3. *F . *F . *F The accession \# of our gene is the following (a part of the description in *F the Genebank) and the cytology is 96B. *F ACCESSION AB036834 *F KEYWORDS MAP kinase phosphatase. *F SOURCE Drosophila melanogaster cDNA to mRNA. *F ORGANISM Drosophila melanogaster *F REFERENCE 1 (bases 1 to 4417) *F AUTHORS Niwa,R., Takeichi,M. and Uemura,T. *F TITLE a novel MAP kinase phosphatase (Drosophila) *F JOURNAL Published Only in DataBase (2000) In press *F Under these circumstances, should we still give you a symbol other than *F MKP? If so, we would think of a tentative symbol. *F Thank you for your time. *F Sincerely, *F With best regards, *F Tadashi Umeura *F From rd120@gen.cam.ac.uk Thu Mar 09 14:03:57 2000 *F To: tuemura@take.biophys.kyoto-u.ac.jp *F Subject: Re: Helping FlyBase: ADRC-643C *F Dear Tadashi, *F Thank you for your prompt reply *F . *F . *F The information you sent me is very useful. It has allowed me (thorugh *F the accession number) to discover that we already have a record for *F your gene in FlyBase, though it is very short: *F \*a MAP-kinase-phosphatase *F \*z FBgn0029157 *F \*g AB036834; BAA89534 *F \*E FBrf0124785 == Niwa, 2000.1.12, GenBank/EMBL/DDBJ: AB036834 *F \*F protein phosphatase ; GO:0004721 ; EC:3.1.3.- | inferred from sequence similarity *F \*Y MAP kinase phosphatase *F \*s Identified with: LD17262 *F 'MAP-kinase-phosphatase' is very long for something that is supposed to *F be a short symbol. Might I suggest Mkph as an abbreviation? That would *F distinguish it from Mkp (FBgn0015764). *F . *F . *F With best regards, *F Rachel. *F From tuemura@take.biophys.kyoto-u.ac.jp Fri Mar 10 04:14:44 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-643C *F Dear Rachel, *F Thank you for your reply. *F We would accept Mkph as an tentative abbreviation of our gene. As soon as *F we determine a formal name of the locus and publish our first paper, we *F will let you know. *F With best regards, *F Tadashi Uemura # *U FBrf0126869 *a Berg *b C. *t 2000.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:49:57 2000 *F To: berg@genetics.washington.edu *F Subject: Helping FlyBase: ADRC-364C *F Dear Celeste, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Genetic and molecular characterization of twin peaks, a novel *F allele of tramtrack with an effect on dorsal follicle cell migration *F in oogenesis.' *F You mention a new allele of ttk, which you call twin peaks. This is *F new to FlyBase and we need a symbol for it. Unless you have another *F symbol in mind I will use ttktp as the allele symbol, keeping 'twin *F peaks' as the allele name. *F Thank you for your help, *F with best wishes, *F Rachel. *F From berg@genetics.washington.edu Tue Mar 07 15:00:02 2000 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-364C *F Dear Rachel: *F After conferring with my student, Rachael French, we prefer the symbol *F ttk-twp, if possible. This particular female sterile line came from the *F Spradling collection, the big screen of 1989 in which I participated as *F a postdoc. Thus, the insertion is a PlacZ, ry+ element (P{PZ}), and the *F Spradling lab number is fs(3)07223. *F Thank you for all your efforts. *F Celeste # *U FBrf0126870 *a Gould *b A. *t 2000.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:59:07 2000 *F To: a-gould@nimr.mrc.ac.uk *F Subject: Helping FlyBase: ADRC-499C *F Dear Alex, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A mutation affecting the migration of larval oenocytes.' *F You write about don, but don't give us a map location - if you could *F that would be nice, as we like to keep things anchored to the map. (We *F ask this map data question to all discoverers of map-position-less new *F genes). Also, we can do a lot more with the data about the mutant if *F you give us a symbol for the mutant. Is it perhaps a BDGP/Szeged line *F and if not is it a PZ/PlacW or what insertion? *F Thank you for your help, *F with best wishes, *F Rachel. *F From agould@nimr.mrc.ac.uk Wed Mar 08 15:51:38 2000 *F Subject: Re: Helping FlyBase: ADRC-499C *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F Phil Elstob (first author of the ADRC 2000 abstract) in the lab has shown *F that the original down-and-out (don) allele is infact a P{A92} insertion *F into svp and fails to complement svpe22 and svpH162. This insertion was *F originally recovered many years ago as the S10 line from an enhancer trap *F screen carried out by myself in collaboration with Rob White and John *F Merriam (unpublished but described in my PhD thesis). We would like to call *F our svp allele svpdon1 with svpS10 as a synonym. *F . *F . *F Best wishes, Alex Gould # *U FBrf0126871 *a VanBuskirk *b C. *t 2000.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:50:55 2000 *F To: cvanbuskirk@watson.princeton.edu *F Subject: Helping FlyBase: ADRC-383A *F Dear Cheryl, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Isolation of a gene involved in the regulation of female germline *F mitosis using a two-hybrid screen with Encore.' *F You mention a gene that is new to FlyBase, identified by a lethal *F P-element line with an insertion site in the 5'UTR. *F Do you have a symbol, name or map location for your gene, and a symbol *F for your mutant allele? Is it one of the large scale genome project *F insertions? These are all data items that we track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From cvanbuskirk@molbio.princeton.edu Thu Mar 09 14:50:41 2000 *F To: Rachel Drysdale (Genetics) *F Subject: ADRC-383A *F Rachel, *F We are naming the new gene from my abstract 'half pint' (no *F hyphen), in keeping with the phenotype. We'll use three letter code of *F 'hfp', if appropriate- this is not used yet in the red book. The map *F location is 62A2-62A4. Do you need any other information? If so, let me *F know. Thanks for prompting me to find a gene name, and thanks for your *F Best Wishes, *F Cheryl # *U FBrf0126872 *a Shi *b W. *t 2000.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:11:35 2000 *F To: jskeath@genetics.wustl.edu *F Subject: Helping FlyBase: ADRC-729B *F Dear Jim, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of flanker, a novel Drosophila gene, expressed in the *F lateral column of the developing CNS.' *F You mention a gene that is new to FlyBase, flanker. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From wshi@sequencer.wustl.edu Wed Mar 08 23:41:24 2000 *F To: Rachel Drysdale *F Subject: Re: Fwd: Helping FlyBase: ADRC-729B *F Dear Rachel, *F This is Weiyang Shi, grad in Jim's lab. The flanker gene (tentative name) *F mapped at the cytological position 65A1-2 and on Celera contig AC014819. *F . *F . *F Best. *F Weiyang # *U FBrf0126873 *a Lazzaro *b B. *t 2000.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:05:08 2000 *F To: bplazzaro@psu.edu *F Subject: Helping FlyBase: ADRC-627B *F Dear Brian, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Variation in Immune Response in Drosophila *F melanogaster at the Molecular and Phenotypic Levels.' *F You mention two genes that are new to FlyBase, AttB and AttC. *F Do you have a map locations for AttB and AttC? These are data items *F that we track, if possible. *F Another abstract, 623A (Testing the usefulness of attacin reporter *F constructs as monitors of 18-wheeler activity in the humoral immune *F response, by Eldon et al.) describes an attacin B. Do you know whether *F this is the same gene as your AttB? If they are the same gene they *F should be represented by one gene record in FlyBase, but if they are *F not they should be represented by two records - which is why I ask. *F Thank you for your help, *F with best wishes, *F Rachel. *F From bplazzaro@psu.edu Thu Mar 09 20:18:50 2000 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-627B *F Dear Rachel, *F I would be happy to provide you with information about Attacin B and *F Attacin C. I first recovered the sequence that I am referring to as *F Attacin B by directly sequencing the product of a low-stringency *F genomic PCR on a line deleted for Attacin A. A BLAST of the recovered *F sequence against the BDGP database showed that the recovered sequence *F lies 1136 bases downstream of the known Attacin A gene. BDGP also *F returned a hit, which I am referring to as Attacin C, in an independent *F contig. BLASTS against the Celera sequence confirmed the sequences of *F the two novel genes and suggested that there are no other Attacin genes *F in the D. melanogaster genome. The BDGP clone containing Attacin C *F apparently maps around cytological position 50A-51A. Att A and Att B *F are arranged head to tail: AttA 5'->3' -- <1.1 kb> -- AttB 5'->3'B. I *F don't know the orientation of AttC relative to AttA/AttB. *F . *F . *F . *F Brian Lazzaro *F _______________________________________________________________________ *F Brian P. Lazzaro (814) 863-7045 office *F Department of Biology (814) 865-2715 lab *F Penn State University (814) 865-9131 fax *F University Park, PA 16802 bplazzaro@psu.edu *F ______________________________________________________________________ # *U FBrf0126874 *a Hou *b S. *t 2000.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:09:25 2000 *F To: shou@mail.ncifcrf.gov *F Subject: Helping FlyBase: ADRC-685C *F Dear Steven, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A novel WD-domain protein organizes FOS/JUN *F transcription complex in regulating dpp expression and epithelial *F cell sheet movement in Drosophila.' *F You mention a gene that is new to FlyBase, medo. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From shou@mail.ncifcrf.gov Thu Mar 09 21:12:22 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-685C *F It was mapped to 28E. *F Steven Hou # *U FBrf0126875 *a Baum *b B. *t 2000.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:55:37 2000 *F To: bbaum@rascal.med.harvard.edu *F Subject: Helping FlyBase: ADRC-441B *F Dear Buzz, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Act-up regulates actin organisation and cell polarity in *F Drosophila.' *F You mention a gene that is new to FlyBase, acu. Do you have a map location for your gene? This is a data class we like to track, if possible. *F Another abstract, number 65: *F Actin-mediated changes in cell shape coordinate the timing of *F Drosophila photoreceptor differentiation. J.E. Treisman , A. *F Benlali , I. Draskovic , D.J. Hazelett. Skirball Institute and Dept. of *F Cell Biology, New York University School of Medicine, New York, NY *F 10016. *F also mentions acu, is this the same acu as yours? *F Thank you for your help, *F with best wishes, *F Rachel. *F From bbaum@rascal.med.harvard.edu Thu Mar 09 21:12:59 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-441B *F Dear Rachel, *F Act-ually, act-up is a name I decdided against. *F I previously presented the gene at the European Drosophila conference as *F capulet. It is the same gene as the Treisman act-up. It is a homologue of *F the *F Drosophila cyclase associated protein, located at 21F. *F Buzz *F Re: the gene symbol -- Since cap is taken I thought *F D-cap or perhaps capt. *F Buzz Baum, *F Dept. of Genetics, *F Harvard Med. School, HHMI, *F 200 Longwood Ave., *F Boston, *F MA 02115. *F tel:617 432 7548 *F fax:617 432 7688 # *U FBrf0126876 *a Cavener *b D. *c P. *d Zhang *e N. *f Geething *t 2000.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:41:44 2000 *F To: dcavener@ctrvax.vanderbilt.edu *F Subject: Helping FlyBase: ADRC-60, 311A and 632A *F Dear Doug, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstracts: *F 'PERK, a newly discovered eIF-2 alpha kinase is a ER *F transmembrane protein.' *F 'ER stress induces phosphorylation of eIF-2 alpha, *F repression of protein synthesis, and induction of ER chaperone genes.' *F and *F 'Signal transduction pathway mediating the endoplasmic *F reticulum unfolded protein response: IRE1 and the ER chaperones.' *F You mention two genes that are new to FlyBase, Perk and Ire. *F Do you have a map locations for Perk and Ire? This is a data class we *F like to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From douglas.cavener@vanderbilt.edu Fri Mar 10 14:32:01 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-60, 311A and 632A *F Rachel, *F PERK is located at 83A1-3; Reference, Unpublished Zhang, P. and Cavener, D.R. *F IRE1 is located at 92B1-B7; Reference unpublished Geething, N. and *F Cavener, D.R. *F We should be submitting sequence/transcript mapping data to Genbank shortly. *F Cheers, Doug *F Douglas R. Cavener *F Department of Molecular Biology *F Box 1820 Station B *F Vanderbilt University *F Nashville, TN 37235 # *U FBrf0126877 *a Liu *b Y. *t 2000.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:49:21 2000 *F To: yliu@welchlink.welch.jhu.edu *F Subject: Helping FlyBase: ADRC-352C *F Dear Yuru, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'jing, a new locus that functions in the *F slbo pathway to control the border cell epithelial to migratory *F transition.' *F You mention a gene that is new to FlyBase, jing. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From yliu@mail.jhmi.edu Fri Mar 10 16:02:37 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-352C *F Hi, Rachel, *F The map location of jing gene is 42B. *F Yuru # *U FBrf0126878 *a Bentley *b M. *t 2000.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 14:49:27 2000 *F To: m-bentley@nwu.edu *F Subject: Helping FlyBase: ADRC-402B *F Dear Meg, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Mutations in a novel gene, ida, cause prepupal lethality *F and defects in chromosome condensation and mitosis.' *F You mention a gene that is new to FlyBase, ida. *F Do you have a map location for ida? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From m-bentley@nwu.edu Fri Mar 10 16:31:09 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Helping FlyBase: ADRC-402B *F Rachel *F it turns out that ida maps to 63Fb. the genetics for this locus are in *F Flybase, but no molecular information. it also turns out that ida has high *F similarity to a subunit of the anaphase promoting complex. i discovered *F this similarity the week following abstract submissions. if you need any *F more information, just ask. *F thanks *F meg bentley *F <><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> *F Meg Bentley *F Graduate Fellow *F Northwestern University Medical School *F 303 East Chicago Avenue *F Molecular Pharmacology & Biological Chemistry, Searle 8-557 *F Chicago, Illinois 60611 *F email: m-bentley@nwu.edu *F phone: 312-503-0963 *F fax: 312-503-5349 # *U FBrf0126879 *a Graham *b P. *t 2000.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:47:21 2000 *F To: pschedl@molbio.princeton.edu *F Subject: Helping FlyBase: ADRC-310C *F Dear Paul, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of Sxl mediated translational control.' *F You mention a gene that is new to FlyBase, Bka. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. Also, does Bka have a full name? If so, now *F would be a good time to record it. *F I am writing to you because the first author does not have an address *F in FlyBase - please feel free to pass my query on if there is someone better *F placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From pgraham@molbio.princeton.edu Fri Mar 10 17:15:48 2000 *F To: ''rd120@gen.cam.ac.uk'' *F Subject: new gene *F Dear Rachel, *F You (relatively) recently wrote to Paul concerning a gene we mention in *F the abstract entitiled 'Analysis of Sxl mediated translational control.' *F The gene was identified based upon a P insertion strain (BL-P1209). The *F only information we have about the map position is the cytological location *F reported by FlyBase for the P insert (30E1-2). We have called it Bka (or *F Bekka) because the undergraduate who identified this insert as something of *F interest is Rebecca Middleton. I suppose Bekka would be considered the full *F name. *F I hope this answers your questions. If you'd like more information, *F please write or call. *F Sincerely, *F Patricia Graham *F Princeton University *F Laboratory of Molecular Biology *F Lewis Thomas Labs *F Princeton, NJ 08540 *F (609) 258-5003 *F pgraham@molbio.princeton.edu # *U FBrf0126880 *a Hiller *b M. *t 2000.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:40:50 2000 *F To: fuller@cmgm.stanford.edu *F Subject: Helping FlyBase: ADRC-49 *F Dear Minx, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A tissue-specific TAF homolog is required for developmentally *F regulated transcription and spermatid differentiation.' *F You mention a gene that is new to FlyBase, nht. Do you have a map location for your gene? This is a data class we like to track, if possible. *F I have addressed this query to you because the first author of the *F abstract is not in FlyBase, but feel free to pass it along if that is *F more appropriate. *F Thank you for your help, *F with best wishes, *F Rachel. *F From hiller@cmgm.stanford.edu Fri Mar 10 21:32:17 2000 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-49 *F Hello, *F I'm the first author for the abstract. nht (no hitter) maps to *F position 35C... *F Mark *F \-------------------------------------------------------------------- *F Mark Hiller, PhD *F Dept. of Developmental Biology *F 279 Campus Dr. *F Beckman B-300 MC5329 *F Stanford University Med.School *F Stanford CA 94305-5329 *F phone: 650-725-7602 fax: 650-725-7739 *F hiller@cmgm.stanford.edu # *U FBrf0126881 *a Starz-Gaiano *b M. *t 2000.3.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 06 13:28:18 2000 *F To: lehmann@saturn.med.nyu.edu, starz@saturn.med.nyu.edu, gilboa@saturn.med.nyu.edu *F Subject: Re: Helping FlyBase: ADRC-338A and 85 *F Dear Ruth, Michelle and Lilach, *F Thank you for your messages. This reply falls into three parts: *F 1: wun2 *F \------- *F I think wun2 corresponds to the gene we already have in FlyBase as *F l(2)k10201. l(2)k10201 maps next to wun and they are transcribed *F towards each other. In the k10201 insertion mutant: *F \*s @P{lacW}@ is inserted into the @l(2)k10201@ transcription unit, approximately *F \*s 2kb downstream of the @wun@ transcription unit, and transcribed in the *F \*s same direction as @l(2)k10201@, thus the opposite direction to @wun@. The *F \*s product of @l(2)k10201@ is truncated and the expression of @wun@ is down-regulated. *F l(2)k10201 is the B transcription unit in Fig 1 of *F \*x FBrf0091204 == Zhang et al., 1997, Nature 385(6611): 64--67 *F whereas the C transcription unit in Fig 1 is wun. *F Please could you confirm that wun2 = l(2)k10201 for me, then I will *F rename l(2)k10201 as wun2. *F 2: G protein linked receptor *F \---------------------------- *F This is the second gene in Michelle's abstract. I am still not sure *F what to call it. The 'predicted gene Melatonin Receptor (AL031765)' is *F in FlyBase as EG:22E5.11, I believe. But I am reluctant to use this as *F the gene symbol unless you are sure that this is the gene being *F misexpressed. If you let me know the EP identifier for your *F misexpression line then I can lodge the data in a placeholder gene *F called 'EPxyz' for now, making a note that EPxyz may correspond to *F EG:22E5.11. We can merge them later, or not, when things become clearer. *F 3: CK00048 *F \---------- *F I will make a gene record for 'BEST:CK00048' to accommodate the *F existence of this gene. We have other such 'BEST' gene records. BEST *F stands for 'Berkeley EST'. This gene symbol serves as a place holder *F and will be overwritten with your 'proper' symbol when you publish *F further work on the gene. *F Thank you all very much for your help, it is much appreciated. *F Regards, *F Rachel. *F From starz@saturn.med.nyu.edu Fri Mar 10 22:01:10 2000 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-338A and 85 *F Hi, *F In fact wunen 2 is NOT l(2)k10201; wun2 lies on the other side of wunen, *F (about 4kb away) and they are transcribed away from each other. So, wun2 *F is off the map in the Nature paper (but would be to the right). The CE *F allele in the Nature paper, as well the deficiencies in their Genetics *F paper (Zhang et al, 143: 1231-1241, 1996) all appear to affect BOTH *F transcripts. We believe that they are functionally redundant. *F About the G-protein linked receptor: the misexpression line that we are *F using is called EP(1)1631. It turns on a downstream gene which we have *F cloned and not yet named. This gene overlaps with the predicted gene that *F I mentioned, but the two are not identical; in particular, a few pieces of *F the predicted gene are spliced out. It still is predicted to be a *F 7 transmembrane protein and has the same homologies to the G-protein *F coupled receptors. ... *F thanks for your help, *F sincerely, *F michelle # *U FBrf0126882 *a Roote *b J. *t 2000.3.14 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Mar 06 17:58:36 2000 *F Subject: Dp(3;3)Cam's *F To: rd120@mole.bio.cam.ac.uk *F Hiya Rach, *F Kevin Cook asked me send you this info: *F Dp(3;3)Cam31 In(3LR)P93L LD3R, pp 61F7-8;64B10-12 *F Dp(3;3)Cam32 In(3LR)LD12L P93R 64B10-12;64E5-9 *F Dp(3;3)Cam33 In(3LR)P10L LD12R, Ubxbx-34e 64E5-9;65C5-9 *F Dp(3;3)Cam34 In(3LR)LD31L P10R, mwh 65C5-9;67C-D *F Dp(3;3)Cam35 In(3LR)274L LD31R, Ubxbx-34e 67C5-11;69A4-5 *F Dp(3;3)Cam36 In(3LR)P42L 274R, ri in cu sr es ca 69A;70F1-2 *F Dp(3;3)Cam37 In(3LR)ME24L P42R 70F1-2;73C3-D7 *F Dp(3;2)Cam30T Ts(2Lt;3Lt)el-24 + Ts(2Rt;3Rt)TE35B-28, pr pwn cn 90C;93C *F BFN, *F J # *U FBrf0126883 *a Lagueux *b M. *t 2000.3.15 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 10:04:51 2000 *F To: jhoff@ibmc.u-strasbg.fr *F Subject: Helping FlyBase: ADRC-626A *F Dear Jules, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Jak and Toll pathways are both involved in the induction of novel *F complement C3/alpha2-macroglobulin like molecules during the immune *F response of Drosophila.' *F You mention six genes that are new to FlyBase, TepI-TepVI. Do you have *F map locations for any of your Tep genes? This is a data class we like *F to track. *F I am writing to you because the first author does not have an address *F in FlyBase - please feel free to pass my query on if there is someone better *F placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From M.Lagueux@ibmc.u-strasbg.fr Wed Mar 15 08:02:40 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-626A *F Dear Rachel *F .... yes I know the chromosome location for all genes; I *F will send the sequences of cDNAs to EMBL this week; these results are not *F published yet *F Gene P1 clone Chromosome localization *F dTEP I DS00365 35F1-F4 *F dTEPII DS02501 28B1-B4 *F dTEPIII DS02501 28B1-B4 *F dTEPIV DS08491 37F1-F2 *F dTEPV DS08491 37F1-F2 *F dTEPVI: is allready in database has a cDNA NCBI ac.number:Y1116, Crowley *F et al unpublished results. *F Marie Lagueux # *U FBrf0126884 *a McGinnis *b W. *t 2000.3.15 *T personal communication to FlyBase *u *F From mcginnis@ucsd.edu Wed Mar 15 02:11:29 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: The Rx gene *F Rachel, *F You might be interested to know, and curate, that the Drosophila *F prd-type homeobox gene called Rx, reported in *F Eggert et al., 1998 Isolation of a Drosophila homolog of the *F vertebrate homeobox gene Rx and its possible role in brain and eye *F development. Proc. Natl. Acad.Sci. USA 95(5): 2343--2348 *F corresponds to the 'genes' that are designated as E97, and wombat, in *F Flybase. The evidence for this is in the nucleotide and amino acid *F sequences of the homeobox regions of those respective clones. They *F are all identical. *F Although I am always reluctant to comment on matters of nomenclature, *F in my opinion the Rx name deserves to be the real one, since Eggert *F et al actually did some serious characterization of the gene, and all *F of the previous lab names e.g. E97, bk50, PPH17, wombat were based on *F highly fragmentary information...... *F Best, Bill *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F William McGinnis *F Professor of Biology and *F Associate Dean of Natural Sciences Phone 858-822-0458 *F Department of Biology 0349 FAX 858-822-0460 *F University of California, San Diego email: mcginnis@biomail.ucsd.edu *F 9500 Gilman Drive http://www.biology.ucsd.edu/labs/mcginnis *F La Jolla, CA 92093-0349 *F NOTE NEW AREA CODE FOR UCSD, 858- versus 619- *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ # *U FBrf0126885 *a Buchman *b V.L. *t 2000.3.15 *T personal communication to FlyBase *u *F From vlb@st-andrews.ac.uk Wed Mar 15 13:11:32 2000 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 15 Mar 2000 13:11:32 +0000 *F Date: Wed, 15 Mar 2000 13:22:41 +0000 *F From: Vladimir Buchman *F Reply-To: vlb@st-andrews.ac.uk *F Organization: Uni of St Andrews *F X-Mailer: Mozilla 4.5 (Macintosh; I; PPC) *F X-Accept-Language: en,ru *F MIME-Version: 1.0 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-721C *F Content-Transfer-Encoding: 7bit *F Content-Transfer-Encoding: 7bit *F Dear Rachel, *F ... our suggestions about symbols and localisation data (by FISH): *F Dubi-d4 (Drosophila ubi-d4/requiem gene): 2R, 41E *F Dneuro-d4 (Drosophila neuro-d4 gene): X, 12A *F Best wishes, *F Vladimir *F 'Rachel Drysdale (Genetics)' wrote: *F > Dear Vladimir, *F > *F > We are currently curating the abstracts for the upcoming 41st *F > (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F > I am writing in connection with your abstract: *F > *F > 'Members of the d4 gene family in Drosophila melanogaster.' *F > *F > You mention two genes that are new to FlyBase. *F > *F > Do you have symbols or map locations for your genes? Map information *F > is a data class we like to track. We cannot record the existence of *F > your genes without them having symbols. *F > *F > I am writing to you because you are the only author for whom I could *F > find an email address - none of the others have email addresses in the *F > FlyBase people directory (nor do you but I managed to find you in the *F > St. Andrews directory) - but do feel free to pass this along to one of the other authors, if that is more appropriate. *F > *F > Thank you for your help, *F > *F > with best wishes, *F > *F > Rachel. *F > *F Dr. V.L. Buchman *F School of Biology *F University of St Andrews *F Bute Medical Building *F St. Andrews, Fife, KY16 9TS *F Tel: 01334 463525 *F Fax: 01334 463600 *F e-mail: vlb@st-and.ac.uk # *U FBrf0126887 *a van de Vosse *b E. *t 2000.3.16 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Fri Mar 03 09:51:16 2000 *F To: boswell@beagle.colorado.edu *F Subject: Helping FlyBase: ADRC-384B *F Dear Robert, *F We are currently curating the abstracts for the upcoming 41st *F (Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'hoepel is downstream of gurken signaling'. *F You mention a gene that is new to FlyBase, hoepel. *F Do you have a map location for your gene? This is a data class we like *F to track, if possible. *F Thank you for your help, *F with best wishes, *F Rachel. *F From esther@spot.colorado.edu Thu Mar 16 17:00:02 2000 *F To: rd120@gen.cam.ac.uk *F Subject: helping flybase: ADRC-384B *F Dear Rachel, *F In answer to your email to Bob Boswell regarding our abstract titled: *F 'hoepel is downstream of gurken signaling' *F the gene hoepel (hoe) is located in 25B1-25C1 *F sincerely, *F Esther van de Vosse *F Department of Molecular, Cellular and Developmental Biology *F University of Colorado *F Boulder, Co 80309-0347 *F USA *F tel: +1-303-492-2259 *F fax: +1-303-492-6465 # *U FBrf0126888 *a Podos *b S. *t 2000.3.18 *T personal communication to FlyBase *u *F From spodos@midway.uchicago.edu Sat Mar 18 17:05:51 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-123 *F Dear Rachel, *F I answer your questions below: *F >Do you have a map location for your gene? This is a data class we like *F >to track, if possible. *F dSmurf1 maps to 54C-D. *F . *F . *F Steve *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Steven Podos, Ph.D. *F Department of Molecular Genetics and Cell Biology *F University of Chicago *F 920 E. 58th Street *F Chicago, IL 60637 *F (773) 702-2848 # *U FBrf0126889 *a Hariharan *b I. *t 2000.3.17 *T personal communication to FlyBase *u *F From hariharan@helix.mgh.harvard.edu Fri Mar 17 23:05:28 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-19 *F > *F >Dear Iswar, *F > *F >We are currently curating the abstracts for the upcoming 41st *F >(Pittsburgh) Annual Drosophila Research Conference, for FlyBase. *F >I am writing in connection with your abstract: *F > *F >'The rocky gene regulates cell size in vivo and encodes *F >the homolog of the human TSC1 (Tuberous Sclerosis Complex 1) *F >tumor-suppressor gene.' *F > *F >You mention a gene that is new to FlyBase, rocky. Do you have a map *F >location for rocky? This is a data class we like to track, if *F >possible. *F > *F >We already know of a fly homologue of human TSC1, called Tsc1 *F >(FBgn0026317, maps to 95E4--95E5). Do you know that rocky is distinct *F >from Tsc1? *F > *F > *F rocky mutations are loss of function alleles of the Tsc1 gene. *F Iswar Hariharan *F Massachusetts General Hospital Cancer Center *F Building 149, 13th Street *F Charlestown MA 02129, USA *F Phone 617-724-9534 *F FAX 617-726-7808 # *U FBrf0126890 *a Bloomington Drosophila Stock Center *b ?. *t 2000.4.13 *T personal communication to FlyBase *u *F Date: Thu, 13 Apr 2000 11:14:36 -0500 *F To: flybase-updates@morgan.harvard.edu *F *F From: Kevin Cook, Bloomington Stock Center *F *F Subject: P{w[+mW.hs]=sevEP-GAL4.B}7 insertion *F *F The following information accompanied stocks from Todd Laverty, UC Berkeley *F (3/00). *F *F P{w[+mW.hs]=sevEP-GAL4.B}7 is a homozygous viable and fertile insertion on *F the second chromosome. The construction and transformation are described *F in FBrf0125052. This reference also notes that the transposon was *F constructed in pP{W8}, hence the w[+mW.hs] marker. # *U FBrf0126891 *a Bloomington Drosophila Stock Center *b ?. *t 2000.4.13 *T personal communication to FlyBase *u *F Date: Thu, 13 Apr 2000 14:51:38 -0500 *F To: flybase-updates@morgan.harvard.edu *F *F From: Kevin Cook, Bloomington Stock Center *F *F Subject: aop construct insertions *F *F The following info accompanied stocks sent by Todd Laverty, UC Berkeley *F (3/00). *F *F Insertion site homozygous *F P{UAS-aop.ACT}IIa 2 lethal *F P{UAS-aop.WT}Ia 2 lethal *F P{sev-yan[ACT]}IVb 3 viable and fertile *F P{sev-yan[WT]}Ib 3 viable and fertile *F P{GMR-yan[ACT]}VIa 2 viable and fertile *F P{GMR-yan[WT]}Ia 3 viable and fertile *F *F These constructs and transformation are described in Rebay and Rubin, 1995, *F Cell 81(6): 857--866 [FBrf0082515]. # *U FBrf0126892 *a Bloomington Drosophila Stock Center *b ?. *t 2000.4.14 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Nicholas Brown, University of Cambridge *F To: Bloomington Drosophila Stock Center *F Subject: Synonyms, phenotype and map information for UAS-CD2 and twi-CD2 insertions *F Date: 23 January 1995 *F *F Information communicated: *F *F Symbol used by N. Brown Symbol used by Bloomington *F P[UAS-CD2.5, w+] P{UAS-CD2}5 *F P[UAS-CD2.6, w+] P{UAS-CD2}6 *F P[twi-CD2, ry+] P{twi-CD2}*, later changed by FlyBase to *F P{twi-CD2}ODB1 *F *F P[UAS-CD2.5, w+] is a homozygous viable insertion on chromosome 2. *F P[UAS-CD2.6, w+] is a homozygous viable insertion on chromosome 3. *F P[twi-CD2, ry+] is a homozygous viable insertion on chromosome 2. # *U FBrf0126893 *a Bloomington Drosophila Stock Center *b ?. *t 2000.4.14 *T personal communication to FlyBase *u *F Date: Fri, 14 Apr 2000 *F To: flybase-updates@morgan.harvard.edu *F *F From: Kevin Cook, Drosophila Stock Center *F *F Subject: P{GMR-ttk88} and P{sev-ttk88} insertions *F P{UAS-Ras85D.K}5-1 *F P{sevhs-Ras1[N17]}JA1 insertion *F *F The following information accompanied stocks from Todd Laverty, UC Berkeley *F (3/00). *F *F P{GMR-ttk88}AT1 and P{sev-ttk88}AT1 are homozygous viable and fertile third *F chromosome insertions. *F The construction and transformation of these constructs was described in *F FBrf0098364. *F *F P{UAS-Ras85D.K}5-1 is a homozygous viable and fertile second chromosome *F insertion. *F The construct and its transformation are described in FBrf0100097. *F *F The P{sevhs-Ras1[N17]}JA1 insertion is a homozygous viable and fertile *F third chromosome insertion. *F The construct and its transformation are described in FBrf0086863. *F # *U FBrf0126894 *a Bloomington Drosophila Stock Center *b ?. *t 2000.4.17 *T personal communication to FlyBase *u *F Date: Mon, 21 Feb 2000 09:58:04 -0500 *F From: Kevin Cook *F *F From: Kevin Cook, Bloomington Stock Center *F *F Subject: P{UAS-cli.B.I}55.2 and P{UAS-cli.B.II}14 insertions *F *F The following information accompanied stocks donated (2/00) by Nancy *F Bonini, University of Pennsylvania. *F *F The P{UAS-cli.B.I}55.2 is a viable and fertile insertion on chromosome 3. *F *F The P{UAS-cli.B.II}14 is a viable and fertile insertion on chromosome 3. # *U FBrf0126895 *a Bloomington Drosophila Stock Center *b ?. *t 2000.5.15 *T personal communication to FlyBase *u *F From: Kevin Cook, Bloomington Stock Center *F Date: Wed, 03 May 2000 *F *F Subject: UAS constructs from M. Muskavitch *F *F The following information accompanied stocks from Marc Muskavitch, Indiana *F University (4/00). *F *F 1) P{w[+mC]=UAS-kuz.F}DF1 is a homozygous viable and fertile third chromosome *F insertion. *F *F The construction and transformation of P{UAS-kuz.F} were described in *F Fambrough et al., Proc. Natl. Acad. Sci. USA 1996 93(23):13233--13238 *F (FBrf0090545). *F *F 2) P{w[+mC]=UAS-fng.K}JK1 is a homozygous viable and fertile third chromosome *F insertion. *F *F The construction and transformation of P{UAS-fng.K} were described in Kim *F et al. Cell 1995 82(5):795--802 (FBrf0083225). *F *F 3) P{w[+mC]=UAS-Ser.mg5603}SS1 is a homozygous viable and fertile third *F chromosome insertion. *F *F The construction and transformation of P{UAS-Ser.mg5603} were described in *F Speicher et al., 1994 Development 120(3): 535--544 (FBrf0074510). *F *F 4) P{w[+mC]=UAS-Dl::N.act}B2a2 is a homozygous viable and fertile second *F chromosome insertion. *F *F The construction and transformation of P{UAS-Dl::N.act} were described in *F Doherty et al., 1996 Genes Dev. 10(4): 421--434 (FBrf0086385). # *U FBrf0126911 *a Misra *b S. *t 2000.4.12 *T personal communication to FlyBase *u *F Category 6: genes for which there is no hint in any jam page but which *F can be uniquely assigned on the basis of neighbouring genes. NOTE that *F I have not confirmed any of these by clustal etc. Plus three that were *F provided by Leyla last night. *F *F Sima analyzed by taking translation from aa_Adh.dros for all except *F alpha-TRY from aa_embl.dros, deltaCOP from SP, BG:DS04095.3 from NCBI gi) *F and blasting against aa_gadfly.dros on BDGP BLAST server. Those that did *F not match, I tried the nucleic acid sequence from na_embl.dros against *F na_gadfly.dros and then against genomic scaffolds at NCBI. *F *F *F >l(2)35Df *F (1055aa) *F =CG4152|FBan0004152|CT12671|FBan0004152 last_updated:000321(1055aa) *F CG4152 CT12671 from neighbours *F *F >BG:BACR48E02.2(398aa) *F =CG16852|FBan0016852|CT35802|FBan0016852 last_updated:000321(162aa) *F CG16852 CT35802 from neighbours *F *F >BG:DS00180.11(505aa) *F =CG8864|FBan0008864|CT25460|FBan0008864 last_updated:000321(505aa) *F CG8864 CT25460 from neighbours *F *F >BG:DS00180.9(284aa) *F =CG16873|FBan0016873|CT35248|FBan0016873 last_updated:000321(185aa) *F CG16873 CT35248 from neighbours *F *F >BG:DS00929.16(1068bp) *F =no gene in Gadfly, but matches 526-1099 of AE003646 & 257241-257511, *F ~259889-260058, 260801-260852 of AE003645, nothing annotated on these coordinates *F CG15268 CT35215 from neighbours *F *F >BG:DS00929.7(565bp) *F =no gene in Gadfly, but matches 32644-32080 of AE003646, in intron of *F CG15274, n *F o gene annotated there *F CG15274 CT35221 from neighbours *F *F >BG:DS01219.3(449aa) *F =CG15275|FBan0015275|CT35222|FBan0015275 last_updated:000321(102aa) *F CG15275 CT35222 from neighbours *F *F >BG:DS02252.4(332aa) *F =CG18518|FBan0018518|CT42262|FBan0018518 last_updated:000321(332aa) *F CG18518 CT42262 from neighbours (no jam page) *F *F >BG:DS02740.18(6849bp) *F =no gene in GadFly, but matches 238895-237298, *F ~237660-236134, 235733-234208, 240693-239523, 239464-238909, *F 236098-235786, 241038-240969 of AE003650, no gene annotated in this *F region, between CG4440 and BG:DS02740.19 *F CG4440 CT14444 from neighbours *F *F >BG:DS04095.3(381bp) *F =CG4959|FBan0004959|CT15918|FBan0004959 last_updated:000321(458bp)--not by *F blastp *F CG4959 CT15918 from neighbours (NB not in paper only GB) *F *F >BG:DS04862.2(444aa) *F =CG15256|FBan0015256|CT35200|FBan0015256 last_updated:000321(385aa) *F CG15256 CT35200 from neighbours *F *F >BG:DS05639.1(751aa) *F =CG12635|FBan0012635|CT35210|FBan0012635 last_updated:000321(739aa) *F =CG12635|FBan0012635|CT42136|FBan0012635 last_updated:000321(344aa) *F CG12635 CT42136/CT35210 from neighbours *F *F >BG:DS07108.4(540aa) *F =CG18480|FBan0018480|CT42128|FBan0018480 last_updated:000321(550aa) *F CG18480 CT42128 from neighbours *F *F >BG:DS07108.5(294aa) *F =CG18478|FBan0018478|CT42124|FBan0018478 last_updated:000321(294aa) *F CG18478 CT42124 from neighbours *F *F >BG:DS07486.5(1101bp) *F =CG18063|FBan0018063|CT40483|FBan0018063 last_updated:000321(1212bp)--not *F by blastp *F CG18063 CT40483 from neighbours *F *F >BG:DS08340.1(306aa) *F =CG15283|FBan0015283|CT35231|FBan0015283 last_updated:000321(91aa) *F CG15283 CT35231 from neighbours *F *F >l(2)34Fa(270aa) *F =CG16880|FBan0016880|CT35243|FBan0016880 last_updated:000321(224aa) *F CG16880/CT35243 from neighbours *F *F >l(2)35Aa(632aa) *F =CG7480|FBan0007480|CT22993|FBan0007480 last_updated:000321(632aa) *F CG7480/CT22993 from neighbours *F *F >l(2)35Ea(418aa) *F =CG4148|FBan0004148|CT12971|FBan0004148 last_updated:000321(470aa) *F CG4148/CT12971 from neighbours *F *F >l(2)35Fe(296aa) *F =CG5818|FBan0005818|CT18240|FBan0005818 last_updated:000321(296aa) *F CG5818/CT18240 from neighbours *F *F >ms(2)35Ci(382aa) *F =CG18483|FBan0018483|CT42134|FBan0018483 last_updated:000321(382aa) *F CG18483/CT42134 from neighbours *F *F >Tim17(478aa) *F =CG15257|FBan0015257|CT35201|FBan0015257 last_updated:000321(413aa) *F CG15257 CT35201 from neighbours *F *F >wb(3367aa) *F =CG15288|FBan0015288|CT35236|FBan0015288 last_updated:000321(3319aa) *F CT35236 CG15288 from neighbours *F *F >&agr;Try(253aa) *F emb|U04853|DM04853_2 &dgr;Try FBgn0010358 SWISS-PROT:P42276complement(U04853:24 *F 1..1002) *F =CG18444|FBan0018444|CT42017|FBan0018444 last_updated:000321(256aa) *F CG18444 says LB, CT42017 no jam *F *F >&dgr;COP(40aa) *F =CG14813|FBan0014813|CT34626|FBan0014813 last_updated:000321(532aa) *F CT34626 == CG14813 says LB # *U FBrf0126912 *a Bayraktaroglu *b L. *t 2000.4.12 *T personal communication to FlyBase *u *F Here is my Category 10 report; the methods are the same *F as used for the previous categories. *F *F Category 10:genes that were not properly linked even in the *F penultimate XML, but for which a candidate is pretty clear. *F *F CanB FBgn0010014 170 aa = CT13882 CG4209 170 aa *F *F MAPk-Ak2 FBgn0013987 359aa =CT10332 CG3086 359aa *F *F nej FBgn0015624 3190 aa U88570=CT35306 CG15319 3275 aa *F (C-terminus diverges at aa 3146 of nej, needs to be checked/fixed) *F *F Pp2A-29B FBgn0005776 591aa=CG17291|FBan0017291|CT32725| 591aa *F *F Adf1 FBgn0000054 253 aa = CG15845|FBan0015845|CT37731| 253 aa *F *F br FBgn0000210 877 aa=CG11491|FBan0011491|CT36317 880 aa *F (there are mismatches in the middle section, to be fixed.) *F (br has several isoforms) *F *F comt FBgn0000346 745 aa=CG1618|FBan0001618|CT41661| 737 aa *F (7 aa missing from CT41661 between aa34-35 wrt CDS from U09373) *F *F ct FBgn0004198 2175 aa =CG11387|FBan0011387|CT31792| 53 aa *F (by nucleotide alignment to AE003441; translation is severely truncated) *F (Aubrey, you mentioned CG12690; CG12690|FBan0012690|CT35562| is the *F neighboring gene, by alignment CHES-1-like FBgn0029504 120 aa is *F likely to be CG12690|FBan0012690|CT35562| 1161 aa *F CHES-1-like is a partial CDS) *F *F e(r) FBgn0011586 104 aa=CG1871|FBan0001871|CT29800|CT5770| 104 aa *F (Two mRNAs that have alternately spliced 5' UTRs but encode identical *F proteins) *F *F *F lola FBgn0005630 MERGE CG12052 and CG18376 *F lola FBgn0005630 467aa=CG12052|FBan0012052|CT40982| 465aa *F lola FBgn0005630 894 aa=CG18376|FBan0018376|CT40707| 442 aa *F (CT40982 of CG12052corresponds to short form encoded by accession U07606, *F longer by 2 aa but rest perfect match.) *F (The other 4 transcripts of CG12052 differ only in the 5' UTR and all encode a *F 787 aa protein the C-terminus of which is suspect; *F (CT40707 CG18376 corresponds to the C-terminus of the 894 aa long isoform of lola *F (accession U07607), to be fixed.) *F *F *F sol FBgn0003464 1597 aa = CG1391|FBan0001391|CT30735| 671 aa AND *F CG1391|FBan0001391|CT40937| 718 aa *F N-terminus of both translations match sol but both are truncated; structures *F need to be fixed (these two transcripts may need to be merged: have to see *F if there is EST evidence before deciding.) Neither match the short (395 aa) *F isoform of sol. *F *F Sxl FBgn0003659 366aa= CG18350|FBan0018350|CT40521 334aa *F (translation start site is further upstream than the one in CT40521) *F *F jim FBgn0027339 820aa=CG11352|FBan0011352|CT31349| 820 aa *F (check alternate transcript CT31353 when reannotating) *F (Aubrey, you said 'see CT23590.jam': where can I find CT23590.jam?) *F *F SNF1A FBgn0023169 582 aa =CG3051|FBan0003051|CT10258| 582 aa *F *F myo-inositol-1-phosphate-synthase FBgn0025885 565 aa=CG11143|FBan0011143|CT31151 565 aa *F (Aubrey, what is 9bg in your original list?) *F *F &bgr;Try FBgn0010357 253 = CG18211|FBan0018211|CT41196| 247aa *F *F &egr;Try FBgn0010425 256 aa=CG18681|FBan0018681|CT42649| 256 aa *F *F BcDNA:GH06348 FBgn0027580 1181 aa=CG1516|FBan0001516|CT3885|CT40220|CT40222 1181 aa *F (CT3885|CT40220|CT40222 differ only in their 5' UTRs and encode the same CDS.) *F *F BcDNA:GH10333 FBgn0027553 594 aa=CG12152|FBan0012152|CT8453 594 aa *F *F _______________________________________________________________________ *F (BcDNA:LD08743 is a synonym of Eb1) *F (Aubrey and MA: Eb1 and BcDNA:LD08743 have to be split) *F *F There are two adjacent genes annotated as Eb1 (see accession AE003789): *F CG3265 and CG3267. *F *F BcDNA:LD08743 (Acc. AF132560) =CG3265|FBan0003265|CT10989 291aa |CT37737 291aa *F *F 'Eb1' (Acc. AF006654) 93 aa = CG3267|FBan0003267|CT10957| 578 aa *F *F Acc. AF006654 has only a partial CDS that aligns with the last 93 aa *F of CT10957 (CG3267); it is not possible for me to tell if the overall *F structure of CT10957 (CG3267) is correct. *F *F BcDNA:LD08743 must have been automatically assigned to Eb1 because it *F hits the third accession under Eb1, AF006645, which is for 'l(2)4524 PZ *F element flanking sequence.' *F _______________________________________________________________________ *F *F BcDNA:LD14270 is a synonym of Cas *F Cas FBgn0027064 975 aa =CG13281|FBan0013281|CT32567|975 aa *F *F BcDNA:LD24527 is not a valid FlyBase gene, since sequence AF145685 *F was deleted from GenBank (although it was retained in the CDS set.) *F Might be best to keep CG9638 as the valid name. *F (The gene formerly known as BcDNA:LD24527 FBgn0027502) 418 aa = CG9638| *F FBan0009638|CT27244| 418 aa *F *F BcDNA:LD32148 FBgn0027494 163 aa = CG12275|FBan0012275|CT17676| 117 aa *F (CT17676 is 46 aa longer at the N terminus than BcDNA:LD32148) *F *F BG:DS07108.1FBgn0028864 464aa= CG18477|FBan0018477|CT42122| 464aa *F *F bgcn FBgn0004581 245aa=CG17611|FBan0017611|CT38864| 245aa *F *F EG:103B4.2 FBgn0023550 475 aa=CG18031|FBan0018031|CT40356| 491 aa *F (one internal region of mismatch; CG18031 is also 16 aa longer at C-terminus.) *F *F EG:115C2.6 FBgn0025635 467 aa=CG17829|FBan0017829|CT39573| 467 aa *F *F EG:132E8.3 FBgn0024986 160 aa= CG3719|FBan0003719|CT12473| 160 aa *F *F EG:80H7.1 FBgn0025385 281 aa = no match *F (The version of EG:80H7.1 in the CDS set out of date.) *F (EG:80H7.1 CDS aligns to coordinates (8957-8481)(8413-8050) of AE003421, *F There is no gene annotated there. CDS of CG14778/CT34588 is at coordinates *F (9138..9273,9335..9417,9477..9659,9722..9880) of AE003421; The EDGP cosmid *F AL031027 does not have a gene annotated at the corresponding coordinates) *F *F EG:BACN32G11.1 FBgn0027796 410aa= CG18531|FBan0018531|CT42308| 410aa *F *F Elongin-B FBgn0023212 118 aa=CG4204|FBan0004204|CT13840 6aa!!! *F (By alignment of AB007692 to AE003731, Elongin-B=CG4204 but transcript *F and translation have to be fixed. Severe truncation of CT13840!) *F *F kin17 FBgn0024887 244 aa = CG5649|FBan0005649|CT17834| 390 aa *F (kin17 CDS is partial) *F *F Mst57Da FBgn0011668 55 aa=CG9074|FBan0009074|CT26012| 75 aa *F (verified by alignment of Z33647 to AE003753; likely that expanded repeat *F region gave rise to size difference) *F *F WD-40-family-member FBgn0026012 730 aa=CG7392|FBan0007392|CT22739| 734 aa *F (4 aa insert in CG7392; rest matches) *F *F Calo MERGE with poe *F (Alignments sent to AG and MA) *F Calo/poe 5322 aa=CG14472|FBan0014472|CT34171| 5322 aa) *F *F Cdic FBgn0013761 653 aa =CG18000|FBan0018000|CT40242| 246 aa *F (note: part of Cdic is homologous to Sdic) *F *F dlt FBgn0024246 871 aa=CG12021|FBan0012021|CT1747| 871 aa *F (note: JTBR FBgn0025820 152 aa = CG1935|FBan0001935|CT5993| 152 aa, *F dlt and JTBR mRNAs overlap at their 3' ends, maybe this caused *F confusion) *F *F Hrb87F FBgn0004237 386 aa = CG12749|FBan0012749|CT27250|385 aa *F (note:this is also the only CG and only scaffold that Hrb85CD aligns *F with so far, but Hrb87F is correct by gene order; is it possible *F that Hrb85CD and Hrb87F are the same thing? Their DNAs align perfectly. *F Will investigate further.) *F *F *F l(2)37Cc FBgn0002031 203 aa = CG10691|FBan0010691|CT29956| 276 aa *F (CT29956 starts further upstream than known l(2)37Cc CDS, has extra aa in *F one matching stretch (intron-exon boundary needs fixing), and two proteins *F are mismatched at their C termini.) *F *F l(3)70Da FBgn0013563 1006 aa = CG6760|FBan0006760|CT20911| 1006 aa *F *F *F l(3)82Fd FBgn0013576 1270 aa = CG10199|FBan0010199|CT9007| 1325 aa *F (CT9007 has an extra stretch of aa s immediately after aa 1078 of AF125384 *F CDS but rest matches well.) *F *F *F l(3)87Df FBgn0002354 73 aa = CG7620|FBan0007620|CT23225| 110 aa *F (Aubrey: Warning: CDS set sequence S77927 is WRONG: it is a psq-l(3)87Df *F fusion protein!)(CG7620 has additional aa at C terminus wrt CDS of Acc. *F S41484.) *F *F PebIII FBgn0011695 158 aa = CG11390|FBan0011390|CT31804| 124 aa *F (better match than CG9358) *F *F rl FBgn0003256 376 aa = CG12559|FBan0012559|CT34260 65 aa| CT39192 55 aa *F (N terminus only, rest not annotated-heterochromatin region gene) *F *F Scp1 FBgn0020908 185 aa = G15848|FBan0015848|CT40161 172 aa *F (intron-exon structure needs to be fixed, ATG is further 5') *F *F ETH FBgn0028738 203 aa = CG18105|FBan0018105|CT40683| 203 aa *F *F Fer2LCH FBgn0015221 227 aa = CG1469|FBan0001469|CT3604| 227 aa *F *F sut2 FBgn0028562 491 aa = G17975|FBan0017975|CT40087| 438 aa *F (CT40087 starts at 3rd Met wrt CDS of acc. AF199484, missing aa 266-299) *F *F sut3 FBgn0028561 476 aa = CG17976|FBan0017976|CT40089| 476 aa *F *F anon-3Ca FBgn0014096 56 aa = CG18089|FBan0018089|CT40590| 56 aa *F *F aralar1 FBgn0028646 682 aa = CG2139|FBan0002139|CT6974| 682 aa *F *F arginase FBgn0023535 351 aa = CG18104|FBan0018104|CT40671| 235aa *F (diverge after aa 197, fix structure.) *F *F (BcDNA:GH10148 is a synonym of mbf1) *F mbf1 FBgn0026208 145aa = CG4143|FBan0004143|CT13720| 145aa *F *F BG:DS00180.14 FBgn0028939 648 aa = CG18146|FBan0018146|CT40902| 701 aa *F (CT40902 has extra aa between aa21-22 of BG:DS00180.14) *F *F BG:DS02252.3 FBgn0028901 1931 aa = CG18109|FBan0018109|CT40741| 1379 aa *F (CT40741 starts at 2nd Met of BG:DS02252.3, and is missing *F >500 aa at the C terminal) *F *F Dsk FBgn0000500 128 aa =CG18090|FBan0018090|CT40598| 128 aa *F *F EG:152A3.2 FBgn0023541 507 aa=CG3540|FBan0003540|CT11900|507 aa *F *F fs(1)K10 FBgn0000810 463 aa=CG3218|FBan0003218|CT10801|463 aa *F *F Met75Ca no match (see note below) *F >From Category 1: *F Met75Cb(FBgn0028415)=CG18064|FBan0018064|CT40477|FBan0018064 *F (note:CG18064 is incorrectly identified as Met75Ca; sequence *F corresponding to Met75Ca exists but is unannotated. This is by *F alignment of Acc.AJ249253 (which has both genes) to AE003520.) *F *F *F OrfKD FBgn0011820 194 aa=CG18103|FBan0018103|CT40669| 69 aa *F (assignment by DNA alignment, structure has to be fixed; OrfKD is a *F partial CDS derived by PCR) *F *F poe MERGE with Calo *F (Alignments sent to AG and MA) *F *F Sdic FBgn0025801 517 aa = CG9580|FBan0009580|CT17580| 215 aa *F (CG9850 corresponds to the C terminus of Sdic) *F (N terminus of Sdic is homologous to part of AnnX, and C terminus *F is homologous to part of Cdic. The Sdic gene is tandemly duplicated *F approximately 10 times. So this region is a bit tricky.) *F *F *F Snap25 MERGE CG17884|FBan0017884|CT39799 (exon 5 of Snap25) AND *F CG17676|FBan0017676|CT39055 (exon 6 of Snap25) *F (The exons of Snap24 extend over 120 kb acc. to FBrf0098336; I BLASTed the *F pieces of DNA that have individual exons annotated on them and got the following: *F exon 2 U81147 aligns to AE003395 (first coding exon) *F exon 3 U81148 aligns to AE003204 *F exon 4 U81149 aligns to AE003242 *F exon 5 U81150 aligns to AE003379 CG17676 *F exon 6 U81151 aligns to AE002931 CG17884 *F exon 7 U81152 aligns to AE002931 *F exon 8 U81153 aligns to AE003013 *F (All scaffolds above are short.) *F *F *F AlstR FBgn0028961 394 aa= CG2872|FBan0002872|CT9822| 106 aa (EG:121E7.2) *F (not a good BLASTP alignment, but by BLASTN this is the correct region; *F CG2872 is too short; AF163775 aligns to 159497(ATG)-178653(@) *F of AE003428 but GC2872 is annotated for <175964..>178557 of same *F scaffold) *F (MERGE AlstR and GR, will send alignment to MA and AG) *F *F *F Cht4 (incomplete CDS) aligns to the same region of AE003452 *F (complement(284130-284527)) *F as CG3986|CT12499 (complement(join(284288..284314,284377..284505)) *F but there is no amino acid alignment. *F Aubrey: Do you put correspondences in when the structure is so obviously off? # *U FBrf0126913 *a Bayraktaroglu *b L. *t 2000.4.13 *T personal communication to FlyBase *u *F Method: same as Categories 1 and 2; most were resolvable by ClustalW. *F *F Category 9: genes which were properly linked in the penultimate XML but *F are not linked in the final one, and where the CG itself is still present *F and seems as though it should indeed be linked *F *F These should be easy by Clustals of proteins. *F Tho we may need advice on why the link went from the last xml. *F *F *F Abd-B FBgn0000015 =CG10291|FBan0010291|CT28893|CT40560| *F *F acj6 FBgn0000028=CG9151|FBan0009151|CT26176| *F *F anon-67Ea = no match *F hay FBgn0001179 =CG8019|FBan0008019|CT24084| *F (anon-67Ea is an ORF of 170 amino acids that begins immediately *F upstream of hay and overlaps the N-terminal coding region of hay) *F *F Apc FBgn0015589 =CG1451|FBan0001451|CT3529| *F *F Atp&agr; FBgn0002921 =CG5670|FBan0005670|CT17822|CT36459|CT36488|CT36562| *F *F bsk FBgn0000229 =CG5680|FBan0005680|CT40896| *F *F Ca-P60A FBgn0004551 =CG3725|FBan0003725|CT12479|CT40312|CT40314|CT40316 *F *F csw FBgn0000382 =CG3954|FBan0003954|CT37554|CT13063 *F *F eIF-4E FBgn0015218 =CG4035|FBan0004035|CT39424|CT13384 *F *F Fer1HCH FBgn0015222 =CG2216|FBan0002216|CT40862|CT40864|CT40866| *F *F for FBgn0000721 =CG10033|FBan0010033|CT42452| *F (align over 561 aa, most of the C terminus of long isoform not here) *F *F Gel FBgn0010225 =CG1106|FBan0001106|CT40886|CT40884|CT1647 *F (CT40886,CT1647 identical CDS, 6aa longer than Gel in CDS set *F CT40884 starts at aa 51 of Gel in CDS set) *F *F Hnf4 FBgn0004914 =CG9310|FBan0009310|CT26497|CT40906 *F (CT40906= Hnf4 in CDS set, CT26497 is 217 aa longer at the N terminus and *F is missing a stretch of amino acids (no blast match of the 217 aa to known *F proteins)) *F *F Hs2st FBgn0024230 =CG10234|FBan0010234|CT28767| *F (good match for first 296 aa, mismatch at C terminus, to fix.) *F *F ImpL3 FBgn0001258 =CG10160|FBan0010160|CT28577| *F *F mts FBgn0004177 =CG7109|FBan0007109|CT21977| *F *F ogre FBgn0004646 =CG3039|FBan0003039|CT9674|CT40095| *F *F Pak FBgn0014001 =CG10295|FBan0010295|CT28905|CT40904| *F *F Pep FBgn0004401 = CG6143|FBan0006143|CT41974|CT19128| *F (CT19128 CDS starts at aa 24 of CT41974 CDS.) *F (Aubrey/Chris? Note:CT40332 is blank in aa_gadfly.dros) *F *F Pkc53E FBgn0003091 =CG6622|FBan0006622|CT20486|CT42082| *F (CT42082 has an extra 9 aa compared to CT20486) *F *F Pp1&bgr;-9C FBgn0003131 =CG2096|FBan0002096|CT6778| *F (CT6778 CDS starts at 3rd Met of Pp1&bgr;-9C from CDS set.) *F *F Pp1-87B FBgn0004103 =CG5650|FBan0005650|CT17842| *F *F PpY-55A FBgn0003140 =CG10930|FBan0010930|CT30615| *F *F Pten FBgn0026379 =CG5671|FBan0005671|CT40892|CT40894|CT17882 *F *F Ptp99A FBgn0004369 =CG2005|FBan0002005|CT6383| *F (CT6383 missing 4aa in midsection, starts at 2nd Met of Ptp99A) *F _____________________________________________________________________ *F A MESS, ONE CG that has 3 CTs that belong to 3 separate genes: *F all 3 in aa_gadfly.dros *F *F repo FBgn0011701 =CG8045|FBan0008045|CT24072| *F BUT: *F CG8045|FBan0008045|CT24092| matches 14-3-3epsilon FBgn0020238 *F CG8045|FBan0008045|CT24072| matches repo FBgn0011701 *F CG8045|FBan0008045|CT24102| matches nothing known *F *F (all 3 are annotated as '14-3-3epsilon' in GenBank.) *F _____________________________________________________________________ *F *F Shc FBgn0015296 =CG3715|FBan0003715|CT12443| *F *F Src64B FBgn0003501 =CG7524|FBan0007524|CT40878|CT1253| *F *F Trfp FBgn0013531 =CG18267|FBan0018267|CT41393| *F *F ttk FBgn0003870 =CG1856|FBan0001856|CT5673|CT36468|CT36466| *F (CT36466 corresponds to the 643 aa short isoform) *F *F tws FBgn0004889 =CG6235|FBan0006235|CT19500| *F (CT36963 is 57 aa and doesn't match CDS in CDS set; *F check when annotating) *F *F Vha16 FBgn0004145 =CG3161|FBan0003161|CT40117|CT12409|CT10607| (all same CDS) *F *F pk FBgn000309 =CG11084|FBan0011084|CT42406| *F (good alignment for first 1050 aa, last 200 or so need fixing/checking) *F *F Abl FBgn0000017 =CG4032|FBan0004032|CT13380| *F *F Acp95EF FBgn0002863 =CG17924|FBan0017924|CT41862| *F (CG missing first 6 aa) *F *F Actn3 FBgn0015008 =CG8953|FBan0008953|CT25722| *F (incomplete CDS in CDSset) *F *F anon-88Bd FBgn0025554 =CG3321|FBan0003321|CT11157| *F *F CanB2 FBgn0015614 =CG11217|FBan0011217|CT31322| *F *F cpo FBgn0000363 =CG18434|FBan0018434|CT41984| *F (aa 6-450 match well, rest need fixing, cpo is incomplete CDS) *F *F _________________________________________________________________ *F WARNING:inconsistency in pnr CGs: *F pnr FBgn0003117 =CG3978|FBan0003978|CT41948| *F NOTE: CG3978 product in GenBank is 531 aa and matches pnr. *F CG3978 product in aa_gadfly.dros is only 32 aa and does not match pnr. *F Aubrey:CT13235 listed by you is not in aa_gadfly.dros. *F _________________________________________________________________ *F *F Pp1-13C FBgn0003132 =CG9156|FBan0009156|CT26196| *F *F Ret FBgn0011829 =CG1061|FBan0001061|CT1245| *F (Ret incomplete CDS in CDSset, CG ORF longer and starts with the amino *F acids MSATAN.) # *U FBrf0126914 *a Misra *b S. *t 2000.4.14 *T personal communication to FlyBase *u *F Category 7: genes which I think are correctly linked already but there *F is a discrepancy between symbol and FBgn (eg one is missing) *F *F Sima analyzed by taking sequence from aa_embl.dros and from aa_gadfly.dros *F and doing pairwise comparisons at ExPasy's SIM alignment site. FBgns were *F checked at FlyBase. *F *F >Ag5r2(254aa) *F emb|AF077304|AF077304 Ag5r2 FBgn0020508 AF077304:37..801 *F =CG9540|FBan0009540|CT26988|FBan0009540 last_updated:00032(254aa) *F CG9540 CT26988 symbol but no FBgn *F =FBgn0030579, add FBgn0020508 *F *F >Cbp80(800aa) *F emb|AJ238970|DME238970 Cbp80 FBgn0022942 AJ238970:269..267 *F =CG7035|FBan0007035|CT21764|FBan0007035 last_updated:000321(800aa) *F CG7035 CT21764 in final xml as EG:84H4.3 *F =FBgn0022942, FBgn0025614 *F *F >Cyp4p1(126aa) *F emb|U34327|DM34327 Cyp4p1 FBgn0015037 SPTREMBL:Q24125 U34327:2..379 *F =CG10842|FBan0010842|CT30367|FBan0010842 last_updated:000321(513aa) *F CG10842 CT30367 symbol but no FBgn *F =FBgn0033396, add FBgn0015037 *F *F >g(783aa) *F emb|U31351|DM31351_2 g FBgn0001087 complement(U31351:397..2748) *F =CG11197|FBan0011197|CT27980|FBan0011197 last_updated:000321(568aa) *F (match only over 430 of 568aa by SIM) *F CG11197 CT27980 symbol but no FBgn *F =FBgn0030516, add FBgn0001087 *F *F >Lcp65Af(100aa) *F emb|U84752|DMU84752_2 Lcp65Af FBgn0020639 SPTREMBL:P92194 *F join(U84752:425..528,U84752:587..785) *F =CG10533|FBan0010533|CT29553|FBan0010533 last_updated:000321(100aa) *F CG10533 CT29553 symbol but wrong FBgn *F =FBgn0035682, add FBgn0020639 *F *F >Nmdmc(357aa) *F emb|L07958|DMNMDMC_2 Nmdmc FBgn0010222 SWISS-PROT:Q04448 L07958:121..1194 *F =CG18466|FBan0018466|CT40203|FBan0018466 last_updated:000321(303aa) *F CG18466 CT40203 symbol but no FBgn *F =FBgn0037658, add FBgn0010222 *F *F >Oamb(637aa) *F emb|AF065443|AF065443 Oamb FBgn0024944 SPTREMBL:O61730 AF065443:929..2842 *F =CG3856|FBan0003856|CT12841|FBan0003856 last_updated:000321(637aa) *F CG3856 CT12841/CT31666 *F see mail to Akira; Ocr is CG7485/CT22999 *F =change from FBgn0004514 to FBgn0024944 *F *F >RpS14b(151aa) *F emb|M21045|DMRGPS14_3 RpS14b FBgn0004404 SWISS-PROT:P14130 *F join(M21045:1572..1808,M21045:1868..2086) *F =CG1527|FBan0001527|CT3937|FBan0001527 last_updated:000321(151aa) *F CG1527 CT3937 final xml says RpS14a *F =change from FBgn0004403 to FBgn0029982/FBgn0004404 *F *F >plu(174aa) *F emb|AC004321|FBpp0001023 plu FBgn0003114 FLYBASE:FBpp0001023 *F join(AC004321:3606..3621,AC004321:3679..4083,AC004321:4138..4241) *F =CG9183|FBan0009183|CT26230|FBan0009183 last_updated:000321(174aa) *F CG9183 CT26230 symbol but no FBgn *F =FBgn0034492, add FBgn0003114 *F *F >Lsp2(718aa) *F emb|X97770|DMLSP2GEN_5 Lsp2 FBgn0002565 SWISS-PROT:Q24388 X97770:686..2842 *F =CG6806|FBan0006806|CT21107|FBan0006806 last_updated:000321(701aa) *F CG6806 CT21107 currently a duplicate anon-68Ed *F =FBgn0036251, add FBgn0002565 # *U FBrf0126915 *a Nelson *b D.R. *t 2000.4.10 *T personal communication to FlyBase *u *F >From dnelson@utmem.edu Mon Apr 10 14:53:50 2000 *F *F ... *F *F I have been teaching a bioinformatics course here at UT and one task we took *F on as a course was the discovery and assembly of all the mitochondrial *F carriers in Drosophila. We found 44, and there is a report in Genbank of *F another very distant member, but it is very weak. I sent this information *F to Marian and Mark Adams, but it was not updated in the paper, which claims *F only 38 mitochondrial carriers. I attach these sequences for you. *F *F ... *F *F [Note the correspondence between these and CG's was established by *F MA April 15 2000 by matches with aa_gadfly.dros0321] *F =============================================================================== *F March 7, 2000 *F *F This is the completed Drosophila mitochondrial carrier set. There are 44 mito carriers in *F Drosophila. The mito carrier motif is P(hyd)(E,D)(hyd)(hyd)(K,R)X(K,R)(hyd)Q *F where (hyd) = a hydrophobic amino acid. Some variation is allowed as in the second *F motif of seq. 3 below. *F *F *F Mito carrier motifs in the sequences are underlined. There should be three, *F spaced about 100 amino acids apart. *F *F 1. AC014984 comp(11159-11678, 9307-9415, 9050-8759) *F two introns ESTs AI107181, AA698455, AI388998, *F AA696852, AA441566 Sun Young Moon *F >CG1628|FBan0001628|CT4364|FBan0001628 last_updated:000321 *F *F MHGGGTGNNINFVEGLIDFLAGSLGGAAQVYVSQPLDTVKVKLQTFPEAYRGMLDCFLST *F YRKDGVLRGLYAGSVPAVFANVAENSVLFAAYGGCQKFVAFCVGKETAGDLTTVQNACAG *F SLAACFSTLTLCPTELIKCKLQALREMKNFVEPAHPQDIRTPWTLTRYIWRTEGIRGFYR *F GLSSTFLREMPGYFFFFGSYEGTRELLRRDDQSKDDIGPLRTMIAGAIGGVCLWTSTFPA *F DVIKSRIQVKNLNESMFAVGADIVRREGVLALYRGLLPSVLRTIPATATLFVVYEYTKRA *F LSATL* *F *F 2. AC013977 comp(58168-59166) = AC008140, AC009219, *F AC009984, AC009741 no introns ESTs AI258994, AI258107 *F David Nelson *F >CG6608|FBan0006608|CT20552|FBan0006608 last_updated:000321 *F *F MAVTTGSTSEATTTTTPVPRRKHSTREQLHQMLAGGLSAAITRSTCQPLDVLKIRFQLQV *F EPLGKNAAKEGPGALTSKYTSIGQAVKTIYREEGMLAFWKGHNPAQVLSIMYGICQFWTY *F EQLSLMAKQTSYLADHQHLSNFLCGAAAGGAAVIISTPLDVIRTRLIAQDTSKGYRNATR *F AVSAIVRQEGPRGMYRGLSSALLQITPLMGTNFMAYRLFSDWACAFLEVSDRSQLPTWTL *F LGLGASSGMLSKTIVYPFDLIKKRLQIQGFESNRQTFGQTLQCHGVWDCLRLTVRQEGVR *F GLYKGVAPTLLKSSMTTALYFSIYDKLKQVRF* *F *F 3. AC019974 comp(31626-32546) = AC009392 no introns *F ESTs AI513226, AI258372, AI135368, AI106672, AI514125, *F AA803450, AI064545, AI405779, AI388401, AI133912, *F AI063819, AI106827, AI064563, AI135996, AI108894, *F AI107113 David Nelson *F *F colt gene Y12495 = AC019974, AC009392 *F *F Hartenstein,K., Sinha,P., Mishra,A., Schenkel,H., Torok,I. and *F Mechler,B.M. *F The congested-like tracheae gene of Drosophila melanogaster encodes *F a member of the mitochondrial carrier family required for *F gas-filling of the tracheal system and expansion of the wings after *F eclosion *F Genetics 147 (4), 1755-1768 (1997) *F *F MTTTENVSTERKANPVKSFLTGGFGGICNVLSGHPLDTIKVRLQTMPRPAPGEQPLYRGT *F FDCAAKTIKNEGVRGLYKGMSAPLTGVAPIFAMCFAGYALGKRLQQRGEDAKLTYPQIFV *F AGSFSGLFSTLIMAPGERIKVLLQTQQGQGGERKYNGMIDCAGKLYKEGGLRSVFKGSCA *F TMLRDLPANGLYFLVYEALQDVAKSKSETGQISTASTIFAGGVAGMAYWILGMPADVLKS *F RLQSAPEGTYKHGIRSVFKDLIVKDGPLALYRGVTPIMLRAFPANAACFFGIELANKFFN *F IVAPNF* *F *F 4. AC020113 comp(29610-29643, 29538-29076, 28424-29000) *F = AC009849 two introns ESTs AI135358, AI135651, AI404820, *F AI513234, AI944743, AI945049 SUN YOUNG MOON *F >CG4995|FBan0004995|CT16036|FBan0004995 last_updated:000321 *F >CG18626|FBan0018626|CT41430|FBan0018626 last_updated:000321 *F *F MVVDFVAGLLGGAAGVLVGHPFDTVKVHLQTDDPRNPKYKGTFHCFRTIVQRDKFIGLYRG *F ISSPMGGIGLVNAIVFGVYGNVQRLSNDPNSLTSHFFAGSIAGVAQGFVCAPMELAKTRL *F QLSTQVDSGIKFTGPIHCLKYIVKTEGIRGAFKGLTATILRDIPGDYFVSFEYLMRQVET *F PGVAYTLMAGGCAGMSSWLACYPIDVVKTHMQADALGANAKYNGFIDCAMKGFRNEGPQY *F FFRGLNSTLIRAFPMNAACFFVVSWVLDICNAKGGMDSVMHSDQPLTLVNLDNKSQADLE *F ATAPTVEEVVRKIITDNAMSHQYVSTPKDVVHSHYTSSTINIPKESKARLASDCNLK* *F *F 5. AC010580 COMP (45288-45333, 45386-45652, 45716-46131, *F 46244-46409) THREE introns = AC017540, AC010579. *F ESTs AA141259, AA141260 Jianning Tao *F >CG4743|FBan0004743|CT15283|FBan0004743 last_updated:000321 *F *F MAAELGLESAAGSVAIKMQEPVNKLKFFHALVAGGVAGMVVDIALFPIDTVKTRLQSELG *F FWRAGGFRGIYKGLAPAAAGSAPTAALFFCTYECGKQFLSSVTQTKDSPYVHMAAASAAE *F VLACLIRVPVEAKQRSQTLQGNKQSGLQILLRAYRTEGLKRGLYRGFGSTIMREIPFSLI *F QFPLWEYFKLQWTPLTGFDSTPFSVALCGAVAGGISAGLTTPLDVVKTRIMLAERESLNR *F RRSARRILHGIYLERGFSGLFAGFVPRVLWITLGGAFFFGFYDLTTRILGATSTDH* *F *F 6. AC013100 20082-20482, 20573-21011 = AC008360 one intron *F Note: this gene is adjacent to AC012807 (2 genes on fragment) *F ESTs AI238956, AI404603, AI063021, AA695373, AI404222, AI401861, *F AA141396 Yuan Gao *F >CG8790|FBan0008790|CT25340|FBan0008790 last_updated:000321 *F *F MPHQERKSMWFFGGLASVGAAMVTHPLDLIKVTLQTQQGHLSVAQLIPKLAREQGVLVFY *F NGLSASVLRQLTYSTARFGVYEAGKKYVNTDSFGGKVALAGASGLVGGIVGTPADMVNVR *F MQNDVKLPPQQRRNYNNAFDGLVRVYRQEGFKRLFSGATAATARGILMTIGQIAFYDQTK *F IYLLATPYFQDNLVTHFTASLVAGTIATTLTQPLDVLKTRSMNAKPGEFNGLWDIVKHTA *F KLGPLGFFKGYVPAFVRLGPHTIITFVFLEQLRLKFGTLN* *F *F 7. AC017347 comp(23830-24078, 23632-23768, 22686-23213) *F = AC007588, AC007852 two introns no ESTs David Nelson *F >CG8323|FBan0008323|CT24577|FBan0008323 last_updated:000321 *F *F MATSDFVLGGLASVGATFFTNPIEVIKTRIQLQGELAARGTYVEPYKGIVNAFITVAKND *F GITGLQKGLAPALYFQFIINSFRLSIYSEAMERRWMHNRKGEVSYGMGLLWGAIGGVVGC *F YFSSPFFLIKTQLQSQAAKQIAVGYQHAHTSMTDALRQIYSRNGVRGLWRGSVAALPRAA *F LGSGAQIATFGKTKALLVQYDLVTQPTLNSFSAGLIAGSIMSVAITPPDVITTRLYNQGV *F DAEGRGLLYRGWLDCFVKILRSEGVYGMYKGFWANYLRIAPHSTLVLLFFDELVAVRTKY *F SNQ* *F *F 8. AC017347 comp(22017-22264, 21802-21937, 21164-21694 *F = AC007588, AC007852 two introns no ESTs David Nelson *F >CG18327|FBan0018327|CT41605|FBan0018327 last_updated:000321 *F *F MATSDFVLGGVAAMGAGVFTNPVEVIKTRIQLQGELAARGSHAQPYKSVFQAFVTVAKND *F GILGLQKGLAPALCFQFVINSFRLSIYTHAVEKGWVHNNKGEISFAKGMLWGALGGVVGS *F YCASPFFLIKTQLQAQAAKQIAVGYQHQHASMSDAIRKIYRKNGVFGLWRGSLANVSRAT *F VASAVQIAVFGQAKSLLKENGVVTHPTILSFCSGLAAGSFVSLAITPLDVVTTRLYNQGV *F DAQGRGIYYRGWLDCVLTILRSEGVYGLYKGFWPIYLRSAPYSTLVLLFFDELIALREKY *F DLHY* *F *F 9. AC017347 comp(20255-20502, 19249-19788, 19836-19971) *F = AC007588, AC007852 two introns *F ESTs AI530949, AI530942, AI259350 first two ESTs have *F retained an intron David Nelson *F >CG18324|FBan0018324|CT41583|FBan0018324 last_updated:000321 *F *F MTKSDFVLGGTAAMGAVVFTNPIDVVKTRMQLQGELAARGTYVKPYRHLPQAMLQIVLND *F GLLALEKGLAPALCYQFVLNSVRLSVYSNALELGYLQNADGSISFYRGMFFGALGGCTGT *F YFASPFYMIKAQQHAQAVQSIAVGFQHKHTSMMDALLHIYRTNGISGFWRAALPSLNRTL *F VASSVQIGTFPKAKSLLKDKGWITHPVLLSFCAGLSSGTLVAVANSPFDVLTTRMYNQPV *F DEKGRGLMYKGLVDCFTKIWRTEGIHGMYKGFWPIYFRSAPHTTLTFVFFEKLLHLRDRY *F VFSQRRN* *F *F 10. AC017377 comp(130619-131276, 130194-130549) one intron *F no ESTs David Nelson *F >CG18340|FBan0018340|CT41647|FBan0018340 last_updated:000321 *F *F MGKGVNTVFRPAEWDNSEEKERPKLEYLVTNKKTPPVELYLTAFASACSAEIVGYPFDMC *F KTRMQIQGEIASRVGQKAKYRGLLATAMGIVREEGLLKLYGGISAMLFRHSLFSGIKMLT *F YDYMREKMIVPDEDGRPQLSFLGSCISGVLAGATASVLTNPTELIKIQMQMEGQRRLRGE *F PPRIHNVLQALTSIYRTGGVVGLWKGTVPNTWRSALVTIGDVSCYDFCKRFLIAEFDLVD *F NREVQFVAAMTAGVADAILSLPADVVKSRIMNQPTDEQGRGIHYKGSLDCLSRLVREEGF *F LAMYKGFIPYWMRVGPASVVFWMTFEQIRRFRGSEGY* *F *F 11. AC017377 comp(131488-132495) no introns *F EST AI947102 David Nelson *F >CG9064|FBan0009064|CT26034|FBan0009064 last_updated:000321 *F *F MDKAERDYWHLRSLEIEEEPRFPPTNVADPLTARNLFQLYVNTFIGANLAESCVFPLDVA *F KTRMQVDGEQAKKTGKAMPTFRATLTNMIRVEGFKSLYAGFSAMVTRNFIFNSLRVVLYD *F VFRRPFLYQNERNEEVLKIYMALGCSFTAGCIAQALANPFDIVKVRMQTEGRRRQLGYDV *F RVNSMVQAFVDIYRRGGLPSMWKGVGPSCMRACLMTTGDVGSYDISKRTFKRLLDLEEGL *F PLRFVSSMCAGLTASVLSTPADVIKSRMMNQPVDESGKNLYYKNSLDCVRKLVREEGVLT *F LYKGLMPTWFRLGPFSVLFWLSVEQLRQWEGQSGF* *F *F 12. AC020205 8898-9190, 9260-10024, 10091-10257, = AC006496 two introns 33 *F ESTs *F AI517048, AI517037, AI404051, AI238330, AI389263, AI063177 *F AI292677, AI387048, AI404547, AI294348, AI389567, AI388110 *F AI293132, AI403941, AI389245, AI114161, AI387833, AI293024 *F AI386902, AI064405, AI063393, AI403869, AI388005, AI292968 *F AI514217, AI402309, AI134761, AA949677, AA817529, AA948886 *F AI515546, AI533469, AI401873 Robert Moxley *F >CG9090|FBan0009090|CT25968|FBan0009090 last_updated:000321 *F *F MFKSLFDAAQNSTFKSPFTSVNCQSATPTSAPTSTAVVTPTLKDVAPRQLTRNHNIAAAA *F VAEGDSCEFGSNHYFLLCGLGGIISCGSTHTMVVPLDLVKCRLQVDPAKYKSVFTGFRIS *F LAEEGVRGLAKGWAPTFIGYSMQGLCKFGLYEVFKKVYGDAIGEENAFLYRTGLYLAASA *F SAEFFADIALAPMEAAKVKIQTTPGFAKTLREALPKMTAQEGVTAFYKGLVPLWMRQIPY *F TMMKFACFERTVELLYKYVVPKPRADCTKGEQLVVTFAAGYIAGVFCAIVSHPADTVVSK *F LNQAKGASALDVAKQLGWSGLWGGLVPRIVMIGTLTAAQWFIYDAVKVFLRMPRPPPPEM *F PESLKKKLGVTGEQ* *F *F 13. AC018265 gene 1 8643-8701, 9520-9654, 9713-10198, *F 10259-10402, 10456-10600 = AC007889 4 introns *F ESTs AI108449, AI531531, AA804104, AI404803 these cover *F up to the third motif at PFDVVK, and the 3 prime UTR. *F The end of this gene matches a C.elegans EST T01651 at 78% *F identity, thus confirming the end of this gene. David *F Nelson *F >CG18347|FBan0018347|CT41675|FBan0018347 last_updated:000321 *F *F MSSSATIATPLPQPQHQQFALLPKIINGGIAGIIGVTCVFPLDLVKTRLQNQQIGPNGER *F MYNSMFDCFRKTYKAEGYFGMYRGSGVNILLITPEKAIKLTANDYFRHKLTTKDGKLPLT *F SQMVAGGLAGAFQIIVTTPMELLKIQMQDAGRVAAAAKLAGKTVEKVSATQLASQLIKDK *F GIFGLYKGIGATGLRDVTFSIIYFPLFATLNDLGPRRNDGSGEAVFWCSFLAGLAAGSTA *F ALAVNPFDVVKTRLQAIKKADGEKEFKGISDCIT*KTLKHEGPTAFFKGGLCRMIVIAPL *F FGIAQTVYYLGVAEGLLGYQKK* *F *F 14. AC018265 11201-11256, 11319-11453, 11525-12293 *F gene 2 = AC007889, AF145637 ESTs AI945223, *F AI946852 David Nelson *F >CG12201|FBan0012201|CT10663|FBan0012201 last_updated:000321 *F *F MLEQVEQKNQEQKKPQKFNVFPKIINGGVAGIIGVACVYPLDMVKTRLQNQTIGPNGERM *F YTSIADCFRKTIASEGYFGMYRGSAVNIVLITPEKAIKLTANDFFRYHLASDDGVIPLSR *F ATLAGGLAGLFQIVVTTPMELLKIQMQDAGRVAAADRAAGREVKTITALGLTKTLLRERG *F IFGLYKGVGATGVRDITFSMVYFPLMAWINDQGPRKSDGSGEAVFYWSLIAGLLSGMTSA *F FMVTPFDVVKTRLQADGEKKFKGIMDCVNRTLKEEGISAFFKGGLCRIMVLAPLFGIAQM *F FYFLGVGEKILGIERTKSV* *F *F 15. AC009385 97280-97481, 97544-97787, 97852-97984, *F 98056-98388 = AC012807 three introns, no ESTs *F Robert Moxley *F >CG18363|FBan0018363|CT41706|FBan0018363 last_updated:000321 *F *F MPVFDDHFLGDCHDEPEGLLPRWWFGGFASMCVAFAVAPIDIVKTHMQIQRQKRSILGTV *F KRIHSLKGYLGFYDGFSAAILRQMTSTNIHFIVYDTGKKMEYVDRDSYLGKIILGCVAGA *F CGSAFGIPTDLINVRMQTDMKEPPYKRRNYKHVFDGLIRIPKEEGWKALYKGGSVAVFKS *F SLSTCSQIAFYDIIKTEVRKNISVNDGLPLHFLTSLGTSIISSAITHPLDVVRTIMMNSR *F PGEFRTVFQASVHMMRFGVMGPYRGFVPTIVRKAPATTLLFVLYEQLRLHFGICSLGGEK *F YN* *F *F 16. AC012788, AC008286, AC008299 mRNA seq in Genbank *F Y18197 ARALAR1 = AC012788, AC008286, AC008299, AC008317, AC008336 *F >CG2139|FBan0002139|CT6974|FBan0002139 last_updated:000321 *F *F MPLTKSLPNSPSLLKRAGTEKLREVFLKYASIQKNGEHYMTSEDFVRKFLGLFSESAFND *F ESVRLLANIADTSKDGLISFSEFQAFEGLLCTPDALYRTAFQLFDRKGNGTVSYADFADV *F VQKTELHSKIPFSLDGPFIKRYFGDKKQRLINYAEFTQLLHDFHEEHAMEAFRSKDPAGT *F GFISPLDFQDIIVNVKRHLLTPGVRDNLVSVTEGHKVSFPYFIAFTSLLNNMELIKQVYL *F HATEGSRTDMITKDQILLAAQTMSQITPLEIDILFHLAGAVHQAGRIDYSDLSNIAPEHY *F TKHMTHRLAEIKAVESPADRSAFIQVLESSYRFTLGSFAGAVAPTVVYPIDLVKTRMQNQ *F RAGSYIGEVAYRNSWDCFKKVVRHEGFMGLYRGLLPQLMGVAPEKAIKLTVNDLVRDKLT *F DKKGNIPTWAEVLAGGCAGASQVVFTNPLEIVKIRLQVAGEIASGSKIRAWSVVRELGLF *F GLYKGARACLLRDVPFSAIYFPTYAHTKAMMADKDGYNHPLTLLAAGAIAGVPAASLVTP *F ADAIKTRLQVVARSGQTTYTGVWDATKKIMAEEGPRAFWKGTAARVFRSSPQFGVTLVTY *F ELLQRLFYVDFGGTQPKGSEAHKITTPLEQAAASVTTENLDHIGGYRAAVPLLAGVESKF *F GLYLPRFGRGVTAASPSTATGS* *F *F 17. AC018185 = 81% identical to AC020205 mRNA = AF137371 *F >CG4994|FBan0004994|CT15958|FBan0004994 last_updated:000321 *F *F MFSSFFETARNSPFRTPMSMARCDAAAPVVEPQPVEGRQIAAAATPVANQQDSCEFGSTK *F YFALCGIGGILSCGTTHTFVVPLDLVKCRLQVDQAKYKNLVHGFKVTVAEEGARGLAKGW *F FPTLLGYSAQGLCKFGLYELFKVKYAEIIGEENAYLYRTSLYLAASASAEFFADIVLAPF *F EAAKVKIQTIPGYANNFREAVPKMLKEEGVNAFYKGLVPLWMRQIPYTMMKFACFERTVE *F LLYKYVVPKPRADCTKGEQLIVTFAAGYIAGVFCAVVSHPADVVVSKLNQAKGASAISVA *F KSLGFSGMWNGLTPRIIMIGTLTALQWFIYDGVKVALGIPRPPPPEMPASLKAKQH* *F *F 18. AC012929 = Y10618 *F join(5191..5487,5563..5690,5768..6242) *F alternative splicing of 5 prime UTR annotated gene in *F Genbank *F >CG16944|FBan0016944|CT37582|FBan0016944 last_updated:000321 *F *F MGKDFDAVGFVKDFAAGGISAAVSKTAVAPIERVKLLLQVQHISKQISPDKQYKGMVDCF *F IRIPKEQGFSSFWRGNLANVIRYFPTQALNFAFKDKYKQVFLGGVDKNTQFWRYFAGNLA *F SGGAAGATSLCFVYPLDFARTRLAADTGKGGQREFTGLGNCLTKIFKSDGIVGLYRGFGV *F SVQGIIIYRAAYFGFYDTARGMLPDPKNTPIYISWAIAQVVTTVAGIVSYPFDTVRRRMM *F MQSGRKATEVIYKNTLHCWATIAKQEGTGAFFKGAFSNILRGTGGAFVLVLYDEIKKVL* *F *F 19. AC012929 = Y10618 *F join(7347..7667,7744..7871,8169..8643) *F alternative splicing of 5 prime UTR annotated gene in *F Genbank *F >CG1683|FBan0001683|CT4708|FBan0001683 last_updated:000321 *F *F MGDEGGGGGHGKGDLKSFLMDFMMGGVSAAIAKTAVAPIERVKLILQVQEVSKQIAADQR *F YKGIVDCFIRIPKEQGFSSFWRGNLANVIRYFPTQALNFAFKDVYKSVFLGGVDKHKQFW *F RHFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGGNREFNGLIDCLMKVIKSDGPI *F GLYRGFIVSVQGIVIYRAAYFGFYDTCRDFLPNPKSTPFYVSWAIAQVVTTVAGIASYPF *F DTVRRRMMMQSGLKKSEMVYKNTAHCWLVIAKQEGIGAFFKGALSNIIRGTGGALVLALY *F DEMKKYF* *F *F 20. AC018177 COMP(38745-38861, 38252-38684, 38006-38182, *F 37773-37945) three introns ESTs AI110167, AA979538, *F AA951938 Ying Shen *F >CG3476|FBan0003476|CT11691|FBan0003476 last_updated:000321 *F *F MEEVEISTEKKSNPVKSFIAGGVGGMCNVLVGHPLDTIKVRLQTMPTPPPGQPPRYKGVI *F DCAARTFRYEGFRGFYRGISAPLVGVTPIYAVDFAVYAAGKRLFQTDDHIRLTYPQIFAA *F GALAGVCSALVTVPTDRIKVLLQTQTVSNGPLLYNGTIDTAAKLYRQGGIRSLFKGTCAC *F ILRDSPTGFYFVTYEFLQELARKKSANGKISTTSTILSGGTAGIVFWTLAVPFDVLKSRL *F QSAPEGTYKHGIRSVFRNLMATEGPKALFRGILPILLRAFPSTAAVFFGVELTNDLLKA* *F *F 21. AC005709 = AC006243, AC009183, AC009354, AC007884, *F AC018192 Muthiah Kumaraswami no introns no ESTs *F >CG2857|FBan0002857|CT9770|FBan0002857 last_updated:000321 *F *F MPENSVVVQLMQAVGGGIAGAATRTITQPLDVLKIRFQMQVEPVTNHKGSKYRGVIHAFK *F SVYAEEGMRGMFRGHNSGQVLSISYALVQFWSYEQLRSMAHQFDYWRERPFLMFFICGGI *F AGCLGAVAAQPFDVVRTQMVAADPSSRRSQMNTFTGLRKVYKMEGWMGLSRGLPFTLVQV *F FPLVGANFLFYKYLNAAVLMAKPPDQRQEIHGAFLFLNGALSGVLAKMIVYPADLLKKRI *F QLMAFKQERKTFGRNPECPTILGCITTTFREEGIGGFYKGMLPTLLKAGLMSAVYFSIYD *F MFKRHYIAPMKEAEKNRQKLGKH* *F *F 22.AC020327 13228-13367, 13433-14314 one intron *F Swapna Menon *F >CG4392|FBan0004392|CT14260|FBan0004392 last_updated:000321 *F *F MNVPDDFTQKEMQTGLWWRHLVAGGIAGAVSRTCTAPLDRIKVYLQVQTQRMGISECMHI *F MLNEGGSRSMWRGNGINVLKIAPETAFKFAAYEQMKRLIRGDDGSRQMSIVERFYAGAAA *F GGISQTIIYPMEVLKTRLALRRTGQYAGIADAAVKIYKQEGVRSFYRGYVPNILGILPYA *F GIDLAVYETLKRRYIANHDNNEQPSFLVLLACGSTSSTLGQLCSYPLALVRTRLQAQGKT *F IIQGHNWFYRNAIIILPFSLAAAETIANQKRKTQIPLKSSDAHSGEETMTGLFRKIVRQE *F GLTGLYRGITPNFLKVLPAVSISYVVYEYTSRALGIKMS* *F *F 23. AC020102 one intron 2 ESTs *F comp(33652-34401, 35642-35870) Ajit Kulkarni *F >CG5254|FBan0005254|CT16777|FBan0005254 last_updated:000321 *F *F MAGQQHDISHAKRAAFQVLAGGSAGFLEVCIMQPLDVVKTRIQIQATPAPNAAALGEVHY *F NGVFDCFAKMYRHEGISSYWKGIMPPILAETPKRAIKFLVFEQTKPLFQFGSPTPTPLTF *F SLAGLTAGTLEAIAVNPFEVVKVAQQADRQKKMLSTFAVAKGIIQQDGLGFSGLNKGITA *F TMGRNGVFNMVYFGFYHSVKNVVPEYKESHLEFLRKVTIGFLAGTLACFVNIPFDVAKSR *F IQGPQPVPGQIKYRGTLSSMGIVYREEGFRALYKGLVPKIMRLGPGGAILLLVFEYYYDY *F LLHNYS* *F *F 24. AC017981 17489-17642, 19697-19850, 19909-20263, 20652- *F 20814, 21800-21942 four introns no ESTs Allen Sickmier *F >CG8026|FBan0008026|CT6130|FBan0008026 last_updated:000321 *F *F MNPIKAQSTGSPKKFNVFAHVKYEHLVAGVSGGVVSTLILHPLDLIKIRFA*VNDGRTAT *F VPQYRGLSSAFTTIFRQEGFRGLYKGVTPNVWGSGSSWGLYFML*YNTIKTFIQGGNTTM *F PLGPTMNMLAAAESGILTLLLTNPIWVVKTRLCLQCDAASSAEYRGMIHALGQIYKEEGI *F RGLYRGFVPGMLGVSHGAIQFMTYEELKNAYNEYRKLPIDTKL*ATTEYLAFAAVSKLIA *F AAATYPYQVVRARLQDHHHRYNGTWDCIKQTWRYERMRG*FYKGLKASLTRVVPACMVTF *F LVYENVSHFLLARRKRIETKEDASDV* *F *F 25. AC020252 comp(11793-12137, 12455-12941) one intron *F EST AI294955 Ji Ma *F >CG4323|FBan0004323|CT14141|FBan0004323 last_updated:000321 *F *F MSYNQRRIARWYFGGLASSMAAMVTHPIDLIKVLIQTQAEKLSVFQTTRKIVKEQGPLAM *F YNGISASMLRQYTYTLARFGIYSVGSGAMDTSTMAGKTCLAAIAGGIGGFVGAPADLINV *F RLQNDVKLPPEKRRNYKHAIDGLVRITREEGWKNLFNGSSMIALRGAFMTVGQIAFYEQS *F KSQMIKLGMPDYMGTYILASMISSVVATTLTQPIDVVKTRRMNAAPGEYSGLGDVFVKTS *F KEGPLAFFKGYVPSLSRLLPHTVLLFLGLEYLRTHFGYLPEPKQTGAFYRYDDVDD* *F *F 26. AC008182 comp(95417-96317) AC017552 AC009523 no intron *F Ji Ma *F >CG9582|FBan0009582|CT27052|FBan0009582 last_updated:000321 *F *F MATRSEETVRSLAHWQFLAGGLSGFIEIICFHPLDVVKTRMQIQGAHPFGGEVVYTCPLD *F AIVKIYRYEGLSSLWKGIVPPICVETPKRGGKFLMYESLKPYFQFGAPQPTPLTHAMSGS *F MAAILESFLVNPFEVVKITQQAHRGKRLKTLSVVKYIIKHDGYGIKGLYRGITALVARNA *F VFHFGFFGFYNALKDIVPSPEDKTYNILRKVIIAGLASSLACVMSVTLDMAKCRIQGPQP *F VKGEVKYQWTISTIKSTFKEEGFRSLFKGLGAMILRVGPGGAMLLVTYEYLFEFLKSQNI *F \* *F *F 27. AC018172 comp(9696-10092, 10536-11242) one intron *F EST AI946682 Yongkai Mo *F >CG2616|FBan0002616|CT8885|FBan0002616 last_updated:000321 *F *F MGRGPRPRSFTGGGGGGGTGGSGGSGGTSGGNNLKPERSAREDDAINRLTDSKSSHRKLL *F SDPRFQIRPLQQVISACTGAMITACFMTPLDVIKTRMQSQQSPAHKCFFYSNGLMDHLFA *F SGPNGSELASLRQRPQFSSSWDALMKISRHEGLAALWSGLGPTLVSALPSTIIYFVAYEQ *F FKARYLQIYESHYNKRFTGLNVFHTSRDTKKSLPSVVPMMSGVTARICAVTVVSPIELVR *F TKMQAQRQTYAQMLQFVRSVVALQGVWGLWRGLRPTILRDVPFSGIYWPIYESLKQNLGH *F GSQPSFSLSFLAGVMAGTVAAIVTTPFDVVKTHEQIEFGERVIFTDSPARDFGKKSTFSR *F LTGIYRTHGVRGLFAGCGPRLLKVAPACAIMISTFEYSKSFFFHYNVRHHNEALLLDNPK *F DTTVEDDDIE* *F *F 28. AC015137 = AC009740 comp(10280-10858,10969-11292) *F one intron ESTs: AA978681, AA104694, AI389649, AI294952, *F AI294952, AI514635, AI239104, AI402446 Ryan Kendall *F >CG6782|FBan0006782|CT21037|FBan0006782 last_updated:000321 *F *F MDRSAFASLVSPYRRRPWMTEHGAAAADSGQVGLKGIVAGGITGGIEICITYPTEYVKTQ *F LQLDEKGAAKKYNGIFDCVKKTVGERGFLGLYRGLSVLVYGSIPKSAARFGAFEFLKSNA *F VDSRGQLSNSGKLLCGLGAGVCEAIVAVTPMETIKVKFINDQRSGNPKFRGFAHGVGQII *F KSEGISGIYKGLTPTILKQGSNQAIRFFVLESLKDLYKGDDHTKPVPKLVVGVFGAIAGA *F ASVFGNTPLDVVKTRMQGLEASKYKNTAHCAVEILKNEGPAAFYKGTVPRLGRVCLDVAI *F TFMIYDSFMDLFN* *F *F 29. AC010671 gene 3 introns shown by \* *F EST AI389787 *F >CG14208|FBan0014208|CT33821|FBan0014208 last_updated:000321 *F *F MDPLRLTTLILSADPRYRIKPMQQVVSALVGGLITTFV*VTPLEVVKTRVQTQHAIRQRP *F TVSKLCYVYHNGLMTHVCRSSDICVPKPGRDPQNLRPLRGAM*DAFVKIVCTSGFSGLWA *F GLSPTLVSALPSTIIYFLTYEYIKNSLSHIYLVSQYYVPMASGICSRTIVVTAITPIEMV *F RIKMQSEYMTYAELWRVLRSLIRQHGILGLWRGWPPTVMRDAPFSGTYWAVYEAIKRAFS *F VTEPTFLFSFLTGAISGAVATFVTMPFDLITTHTQIELGQDVL*PSVLSRMRQIYRLQGV *F RGLYVGVMPRMLRVVPACAIMISTFEYSKSFFFHYNLDLQEAGTYYVKCQ* *F *F 30. AC010671 gene 3 introns shown by \* *F EST AI402433 *F >CG14209|FBan0014209|CT33822|FBan0014209 last_updated:000321 *F *F MAAASSQNPSKATMTDPRFRIRPLQQVASACTGAMVTACF*MTPLDVIKTRLQAQQQALL *F SNKCFLYCNGLMDHICPCGPDTPNPAAAKPAPRFSGTI*DAFIKISRTEGIGSLWSGLSP *F TLISALPSTIIYFVAYEQFKARFTDIHHPIPFLVPLLAGVSGRILAVTCVSPVELIRTKM *F QSQRMTHAEMFGTIRQVVQSQGVLGLWRGLPPTILRDVPFSGIYWTCYEYLKSSFGVVEP *F TFSFSFAAGAISGSVAATITTPFDVVKTHEQIEFGEKFIFS*DNPPKQVATKSVAMRLAS *F IYRMGGVPAIFSGLGPRLFKVAPACAIMISSFEYGKSFFYHYNIDQHNRSNQATKGPGS* *F *F 31. AC019585 = AC009388, AC009249 introns shown by \* *F ESTS AI064276, AA439218, AI386586, AA698575, AI946686, *F AI945569 David Nelson *F >CG4963|FBan0004963|CT15898|FBan0004963 last_updated:000321 *F *F MNIDDYESLPTTSVGVNMTAGAIAGVLEHVVMYPLDSVKVR*MQSLSPPTKNMNIVSTLR *F TMITREGLL*RPIRGASAVVLGAGPAHSLYFAAYEMTKELTAKFTSVRNLNYVI*SGAVA *F TLIHDAISSPTDVIKQRMQMYNSPYTSVVSCVRDIYKREGFKAFYRAYGTQLVMNLPYQT *F IHFTTYEFFQNKMNLERKYNPPVHMAAGAAAGACAAAVTTPLDVIKTLLNTQETGLTRGM *F IEASRK*IYHMAGPLGFFRGTTARVLYSMPATAICWSTYEFFKFYLCGLDADQYKSSITG *F SSEPRKADYVLPRTTDEEQIDQEREAAKEKDTTATLHSAPTSVNASGAIKTVCELSTRPA *F GPTINLHTRHTDVKSPYERGFST* *F *F 32. AC012986 30851-31404, 31468-31597, 31665-31979 *F = AC007886, AC009733 two introns ESTs AI404722, *F AI516330, AI292585, AI542291 David Nelson *F >CG7943|FBan0007943|CT5716|FBan0007943 last_updated:000321 *F *F MKDDGAPNVAKTTDPPPPKRFPSGRAHSPHGDGEAGKLLHGSVFSKRFFGSFQWEEFACG *F CGAAFVNIAVTYPIYKMIFRQMLHGVPITSAFAQLRHEGLGFLYRGMLPPLAQKTISLSI *F MFGVVDGTRRYLVEDYRLNDYGAKVLAAVVAGSAESILLPFERVQTLLADSKFHQHFSNT *F QNAFRYVVSHHGYRELYRGLEPVFWRNGLSNALFFVLREEASVRLPKRKSVSTRTVQEF *F IAGAVIGASISTIFYPLNVIKVSLQSEMGQRSEGSWQACKRIYVERDRRIGNFYRGCPFN *F TGRSFISWGIMNTAYENLKKLMQQQPPLPLASE* *F *F 33. AC017153 = AC004442 EST AI259170 5 introns *F David Nelson *F >CG18317|FBan0018317|CT41569|FBan0018317 last_updated:000321 *F *F MAQNTADTLIHLIAGG*SAGTVGAVVTCPLEVVKTRLQSSTAFMTPSRLAENAGGGPANG *F GQSELLRPEQRRKLSTTILRNRSQPQ*IMAISHCGISSTTPKSMSIVQCL*RHIVQNEGP *F RALFKGLGPNLVGVAPSRAIYFCTYSQTKNTLNSLG*FVERDSPLVHIMSAASAGFVSST *F ATNPIWFVKTRMQLDYNSKVQMTVRQCIERVYAQGGVAAFYKGITASYFGICETMVHFVI *F YEFIKSKL*LEQRNQRHTDTKGSRDFLEFMMAGAVSKTIASCIPYPHEVARTRLREEGNK *F YNSFWQTLHTVWKEEGRAGLYR*GLATQLVRQIPNTAIMMATYEAVVYVLTRRFNNKSNE *F FYDF* *F *F 34. AC005445 COMP(19104-19517, 18486-18939) one intron *F EST AI944701 Brian Bothner *F >CG11196|FBan0011196|CT31268|FBan0011196 last_updated:000321 *F *F MGEDSSRRLPRWWFGGVCAAIAVTGTHPIDLIKVQLQTQSQADRKTVGEILKGIHERSGI *F LGFYNGISASWFRQLTYTTTRFALYEAGKDYVDTQKVSSKMALATFAGIVGGIVGVPGDV *F VTVRLQNDVKLPEEKRRSYKHVFDGLFRIYKEEGVSSLFRGTVPAVSRAVLLTIGTNAAY *F DQVKQMLKIATGAGEGVPLHFATSTIAGCIAVVITQPLDVIKTTFMNAQPGEFSGIGGAF *F LSTAKQGPLAFYKGFIPALIRVSPNTIITFVLYEQARMRFGYLPPDK* *F *F 35. AC019775 comp(52852-53040, 53240-53367, 54125-53426) *F = AC008205 2 introns ESTs AA697685, AA697686, AI402846, *F AI402855, AI403013 David Nelson *F >CG5805|FBan0005805|CT18202|FBan0005805 last_updated:000321 *F *F MTESSSSTKSRLAVAPTGVGGAAEGATYIRTIEWDMMNKTKFFPLSMLSSFSVRCCLFPL *F TVIKTQLQVQHKSDVYKGMVDCAMKIYRSEGVPGLYRGFWISSVQIVSGVFYISTYEGVR *F HVLNDLGAGHRMKALAGGGCASLVGQTIIVPFDVISQHAMVLGMSAHAGSKGDINPLGIK *F SWPGRSRLHISMDIGREIMRRDGFRGFYRGYTASLMAYVPNSAMWWAFYHLYQQELFRIC *F PVWSHLFIQCVAGSLGGFTTTILTNPLDIVRARLQVHRLDSMSVAFRELWQEEKLNCFFK *F GLSARLVQSAAFSFSIILGYETIKRIAVDEQYKHQIRW *F *F 36. AC017782 comp(21596-21725, 20691-21524) = AC007856 *F AC008218 one intron shown by \* ESTs AI512269, AI405068, *F AI260461, AI518659, AA697477, AI403621, AI297023, *F AI455634, AA803073, AA697231 David Nelson *F >CG1907|FBan0001907|CT2867|FBan0001907 last_updated:000321 *F *F MSATSVQEAPKKAVATNAIKFLFGGLSGMGATMVVQPLDL*VKTRMQISGAGSGKKEYRS *F SLHCIQTIVSKEGPLALYQGIGAALLRQATYTTGRLGMYTYLNDLFREKFQRSPGITDSM *F AMGTIAGACGAFIGTPAEVALVRMTSDGRLPVAERRNYTNVANALARITREEGLTALWRG *F SLPTVGRAMVVNMTQLASYSQFKTYFRHGPLQMEEGIKLHFCASMLSGLLTTITSMPLDI *F AKTRIQNMKMVDGKPEYRGTADVLLRVARQEGVFALWKGFTPYYCRLGPHTVLTFIILEQ *F LNQGYNKYVLGSNKSTGL* *F *F 37. AC020238 = AC007756 AC007757 three introns shown by \* *F no ESTs David Nelson *F >CG4241|FBan0004241|CT13930|FBan0004241 last_updated:000321 *F *F MSLRLFIHSRAFGYECILRFSIHFLPFTISVRQKIDQVVISLISGAAAGALAKTVIAPLD *F RTKINFQIRNDVPFSFRASLRYLQNTYANEGVLALWRGNSATMARIVPYAAIQFTAHEQW *F RRILHVDKDGT*NTKGRRFLAGSLAGITSQSLTYPLDLARARMAVTDRYTGYRTLRQVFT *F KIWVEEGPRTLFRGYWATVLGVIPYAGTSFFTYETLKREYY*EVVGNNKPNTLVSLAFGA *F AAGAAGQTASYPLDIVRRRMQTMRVNTAGGDRYPTILETLVKIYRC*EGVKNGFYKGLSM *F NWIKGPIAVGISFSTYDLIKAWLTELANLRRVEK* *F *F 38. AC014153 GENE 1 comp(17446-18381) = AC010665 *F ESTs AI405292, AI134015, AI389947 *F >CG18418|FBan0018418|CT41906|FBan0018418 last_updated:000321 *F *F MALVYGVEKKTVPTHMKFVMGGTSGMLATCIVQPLDLLKTRMQISGTLGTREYKNSFEVL *F SKVLKNEGILSLYNGLSAGLLRQATYTSAKMGVYQMELDWYRKNFGNYPSMVASMTMGIV *F AGAFGAMCGNPAEVALIRMMSDNRLIPEDRRNYKNVGDAFVRIVKDEGVVALWRGCLPTV *F GRAMVVNMVQLASYSLMKNQLHGYLSEGIPLHLTAALVSGLLTSVTSMPLDMAKTRIQQM *F KVIDGKPEYSGTIDVLKKVLKNEGAFAVWKGFTPYLMRMGPHTIFSFVFLEQMNKAYSKH *F MLSDSLSDSVP* *F *F 39. AC014153 GENE = 2 comp(16188-17093) no ESTs *F >CG7514|FBan0007514|CT2166|FBan0007514 last_updated:000321 *F *F MAYSIEKKSIPGYMMYINGGLAGMLGTCIVQPLDLVKTRMQISATTGEYKSSFDCLLKVF *F KNEGILALYNGLSAGLMRQATYTTARMGFYQMEIDAYRKQFNAPPTVLASMGMGILAGAF *F GAMFGNPAEVALIRMMSDNRLPPAERRNYTGVLNAFVRIVKDEGVITLWKGCMPTVGRAM *F IVNMVQLASYSQLKAAFSEYFSGLSLHIAAAMMSGLLTTIASMPLDMAKTRIQQQKTAEY *F KGTMDVLMKVSKNEGIASLWKGFTPYLCRLGPHTVFAFIFLEQLTKAYKHIVLGDDSESN *F I* *F *F AC009216 120925-121173, 122098-122207, 122345-122834, *F 122899-123071 = AC012693 45% TO CE5 no ESTs 3 introns shown *F by \* *F >CG6492|FBan0006492|CT20203|FBan0006492 last_updated:000321 *F *F MAAKTDESSPAVASSTSSNPAPSSGRHQLRPVKFDYADSFACTYIVSVVAASIAELATYP *F LDLTKTRLQIQGEGAAHSAGKSN*MQYRGMVATAFGIAREEGALKLWQGVTPALYRHVVY *F \*SGVRICSYDLMRKEFTQNGTQALPVWKSALCGVTAGAVAQWLASPADIVKVQIQMEGRR *F RLMGEPPRVHSAGHAFRQIVQRGGIKGLWKGSIPNVQRAALVNLGDLTTYDTIKHLIMNR *F LQMPDCHTVHVLASVCAGFVAAIMGTPADVVKTRIMNQPTDENGR*GLLYRGSVDCLRQT *F VSKEGFVALYKGFLPCWIRMAPWSLTFWLSFEQIRKMIGASGY* *F *F AC019525 200827-200898, 200975-201035, 201914-202725 *F 2 introns shown by \* = AC010700 *F ESTs AI512559, AA697689, AI259003, AI532563 *F >CG1326|FBan0001326|CT2942|FBan0001326 last_updated:000321 *F *F MVAPSKLSQVLSYQNFVHAVSGAA*GGCIAMSTFYPLDTVRSRLQL*EEAGDVRSTRQVI *F KEIVLGEGFQSLYRGLGPVLQSLCISNFVYFYTFHALKAVASGGSPSQHSALKDLLLGSI *F AGIINVLTTTPFWVVNTRLRMRNVAGTSDEVNKHYKNLLEGLKYVAEKEGIAGLWSGTIP *F SLMLVSNPALQFMMYEMLKRNIMRFTGGEMGSLSFFFIGAIAKAFATVLTYPLQLVQTKQ *F RHRSKESDSKPSTSAGSTPRTESTLELMISILQHQGIRGLFRGLEAKILQTVLTAALMFM *F AYEKIAGTVGMLLKRN* *F *F AC014955 = AC006492 4 introns shown by \* ESTs AA949318, *F AA142277, AI405082, AI114037, AA695733, AI402226, *F >CG7314|FBan0007314|CT22571|FBan0007314 last_updated:000321 *F *F MGEVKDWRPFVYGGVASITAEF*GTFPIDTTKTRLQIQGQKIDQSFSQLRYRGMTDAFVK *F ISREEGLRALYSG*IWPAVLRQATYGTIKFGTYYTLKKLANERGLLINEDGSERVWSNIL *F CAAAAGAISSAIANPTDVLKVRMQVHGKGQHKGLLGCFGEIYKYEGVRGLWRGVGPTAQR *F AVVIASVELPVYDFCKLQLMNAFGDHVGNHFI*SSFIASLGSAIASTPIDVIR*TRLMNQ *F RPVSITMNGVVTAAATPKLYSGSLDCAVQTIRNEGLPALYKGFIPTWVRMGPWNIIFFIT *F YEQLKKY* *F *F AC007817 = AC019578 4 introns shown by \* ESTs AA440846, *F AI542106 *F >CG5646|FBan0005646|CT17838|FBan0005646 last_updated:000321 *F *F MKWENYCDFVAGCFG*GACGVLVAHPLDTIKVWQQASNSSVVTAIQQIYSRNNG*VNGFY *F RGMFFPFISTGAINSLLFGIYGNHLRQLRKVCHSDYQREQLEYHNMFLAGSVAGFVQSFI *F ACPMELIKVRLQTAT*YYSDYLYGQRRTAFGTFKRILKTDGISGLYRGLLPM*IDVLPYG *F IYMLAYRQGVDYMDRRDFVRRRRSQSDGSSVNLLVTTLAGAWAGVISWVCVIPFDVVKTL *F MQADENHKYRGIFHCVRVQYRAYGWRSIFRGSWMLVARAVPFNAATFLGYEYALEWCQRW *F NGTVY* *F *F AC014256 21309-21745, 21866-22307 = AC008356, AC008234 *F one intron shown by \* no ESTs *F >CG16736|FBan0016736|CT32113|FBan0016736 last_updated:000321 *F *F MNNSDAGSSIEQFIKMPVYVGLLIKTTAQLLSHPMELVRVNMQANVIHHSRLSINHMFRL *F MARHGLPGFYYGIVAACLRCTVHTMSTYTLFYNLQDNKYVLMLQPYNTSMVLGITGFWGG *F VLATPFAKLAVIRQADLTRGSYERRK*NYRNFWRGLKCMYAKGGFTYLFTGWKINSISST *F AVAVLYTPISDKVHTVISWFHRLDEPWLSDLITMALTGSIITVIMTPVDALATLTLNESS *F HYGRTSYPYLYRKIIRKHGYKGFFFGWKPALMALIPHTVLATFVYRFLLDRYIT* # *U FBrf0126922 *a Eeken *b J. *t 2000.3.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Mar 29 18:59:35 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Eeken mutations *F From: Kevin Cook, Bloomington Stock Center *F Subject: Eeken mutations *F The following information accompanied stocks donated to the Stock Center *F (3/00) by Jan Eeken, Leiden University. *F 1. l(1)1Bm8-12-2 *F This is a spontaneus, recessive lethal mutation isolated on a mei-9a *F chromosome which maps to 1B5-10. It complements mutations in l(1)1Bb, *F l(1)1Bc, svr, elav and vnd. *F 2. sw20-14 *F This is a spontaneous, recessive lethal mutation in sw isolated on a *F mei-9a chromosome. *F 3. FM7c, ctS balancer variant *F The ctS mutation is present on an FM7c chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0126923 *a Bayraktaroglu *b L. *t 2000.4.17 *T personal communication to FlyBase *u *F Category 8: genes which have been deliberately separated from their CG's *F because there was more than one such CG and we had no time to sort them *F out earlier. Here, then, the requirement is to decide which CG NOT to *F merge with the gene. I have only made a preliminary analysis. *F *F These should be easy by Clustals of proteins. *F *F >Ag5r FBgn0015010 = CG9538|FBan0009538|CT26984| *F *F >And FBgn0011273 = CG17769|FBan0017769|CT39380|FBan0017769 *F DO NOT MERGE *F (And FBgn0011273 does not align with CG17770|FBan0017770|CT39382|. *F CG5024|CT16147 is NOT And by alignment, but all 3 seem to be *F Calmodulin-related) *F *F *F >Cnx99A FBgn0015622 = CG11958|FBan0011958|CT4036| *F (CG9906|FBan0009906|CT27896| most likely Cnx14D; it's clearly related to *F but not the same as Cnx99A) *F *F >drl FBgn0015380 = CG10758|FBan0010758|CT30136| AND *F CG17348|FBan0017348|CT30099| *F MERGE *F *F >Fur1 FBgn0004509 = CG10772|FBan0010772|CT29938| *F (CG17049 has no homology to known fly genes (by BLASTN and BLASTP at NCBI)) *F *F >fz FBgn0001085 581 aa = CG3646|FBan0003646|CT12089|329aa (fz aa 1-329) AND *F CG17697|FBan0017697|CT39176| 166aa (fz aa 416-581) *F MERGE *F (aa 330-415 still missing! fz spans over 90 kb, 60 kb gap OK, some of the *F missing aas are probably in the gap) *F *F >lds FBgn0002542 974aa = CG2684|FBan0002684|CT9083| 1061aa *F DO NOT MERGE *F (CG10445|FBan0010445|CT29324 is not lds, see Clustal; *F Aubrey, the two CG's don't seem to be overlapping in the GenBank annotation, *F do you have additional info?) *F *F *F >Lk6 FBgn0017581 = CG6929|FBan0006929|CT21460| AND CG17342|FBan0017342|CT21464| *F MERGE *F *F *F >Mgstl FBgn0025814 = CG1742|FBan0001742|CT5060| *F Mgstl must be CG1742: CG12628/CT35150 is on wrong arm *F (CG12628 is probably a related protein) *F *F >Pdp1 FBgn0016694 = CG17888|FBan0017888|CT39809| *F (Rab-RP4 FBgn0015794 =CG3129|FBan0003129|CT10657|) *F *F >ras FBgn0003204 = CG11485|FBan0011485|CT36321| AND CG1799|FBan0001799|CT5398| *F MERGE *F *F >RpS14a FBgn0004403 = CG1524|FBan0001524|CT31809| *F (RpS14b FBgn0004404 = CG1527|FBan0001527|CT3937|) *F *F >RpS15A *F ?? *F 'RpS15A must be CG12324: CT6271/CT5068 CG2033 wrong arm' *F CONFLICT: both are good matches to RpS15A. By nucleotide alignment *F RpS15A cDNAs align better to where CG2033 is on scaffold. Could cytology *F have been derived from location of CG12324? See Clustal. *F *F >ScpX FBgn0015808 = CG17320|FBan0017320|CT29258|CT29260 best match *F DO NOT MERGE *F (CG17597 looks like it's a adjacent related gene, but both ScpX cDNAs *F in GB match CG17320 better by nucleotide alignment) *F *F >sif FBgn0019652 = CG5256|FBan0005256|CT16789| AND CG5406|FBan0005406|CT17116 *F MERGE *F *F >Taf80 FBgn0010356 = CG7704|FBan0007704|CT23475|F *F DO NOT MERGE *F (CG6751 also annotated as Taf80 probably because of nucleotide sequence *F overlap, its protein does not align with GB Acc. U06460 for Taf80) *F *F >term FBgn0003683=CG4216|FBan0004216|CT13826| *F DO NOT MERGE,SUSPECT *F (term matches CG4216 throughout its length (428) but matches only the *F first 261 aa of CG7271 which is on the other strand. Adjacent *F related gene?) *F *F *F >anon-68Ed FBgn0017424 = CG11538|FBan0011538|CT17864| *F *F >BcDNA:GH07188 FBgn0027578 = CG14526|FBan0014526|CT34253| *F DO NOT MERGE *F (By nucleotide alignment CG14526 is BcDNA:GH07188; this is probably *F a multigene family) *F *F *F >BcDNA:LD21735 834 = CG18617|FBan0018617|CT22945| AND *F CG7679|FBan0007679|CT24090 |CT30413 *F MERGE *F *F *F >BG:DS00929.8 FBgn0028922 = CG4182|FBan0004182|CT12287| *F (l(2)35Bg FBgn0001977 = CG4180|FBan0004180|CT13516|) *F *F >JTBR FBgn0025820 152 aa = CG1935|FBan0001935|CT5993| 152 aa *F >dlt FBgn0024246 871 aa = CG12021|FBan0012021|CT1747| 871 aa *F DO NOT MERGE *F (from Category 1 list) *F *F >l(2)dtl FBgn0013548 = CG11295|FBan0011295|CT31521| *F DO NOT MERGE *F l(2)tid(FBgn0002174) = CG5504|FBan0005504|CT17450|FBan0005504 *F (note: CG5504 is erroneously annotated as l(2)dtl in public view of GadFly; *F CG11295 is correctly annotated as l(2)dtl) *F (from Category 1 list) *F *F *F >Lnk FBgn0028717 = CG5908|FBan0005908|CT18543| AND CG17367|FBan0017367|CT38370| *F MERGE *F *F >Pp4-19C = CG1596|FBan0001596|CT3517 AND CG1459|FBan0001459|CT3537| *F MERGE *F *F >RpL8 FBgn0024939 = CG1263|FBan0001263|CT2579|CT37695 *F DO NOT MERGE *F CG18334|FBan0018334|CT37687 is NOT RpL8!!! *F *F Uch FBgn0010288 = CG4265|FBan0004265|CT10917| *F *F rost FBgn0011705 = CG9552|FBan0009552|CT27002| *F (CG9555 is adjacent, related, do not merge.) *F *F Rbp9 = CG3151|FBan0003151|CT10570|CT38165| *F (CT10570 extends 229 aa beyond Rbp9 from CDS set, check when annotating.) *F (CG5808 is not Rbp9.) *F *F fw FBgn0001083 = CG1500|FBan0001500|CT3745| *F (CG1500 has more than 150 aa upstream of the start of fw in CDS set, *F and is missing some of the C terminus of the fw in CDS set. *F CG9095 has some identity with fw but is not fw.) *F *F &agr;-Adaptin FBgn0015567 = CG4260|FBan0004260|CT13966| *F (CG9113 is not &agr;-Adaptin.) *F *F Acp33A FBgn0023414 = CG15841|FBan0015841|CT40022| AND CG6555|FBan0006555|CT20399| *F (MERGE: Acp33A has 2 possible ORFs on one mRNA, these are the 2 ORFs.) *F *F nAcR&bgr;-64B FBgn0000038 = CG12606|FBan0012606|CT34874| AND CG11348|FBan0011348|CT31654| *F (MERGE; CG11348 and CG12606 start at the same ATG but CG11348 doesn't splice out *F the first intron and CDS truncates within the intron) *F *F Mst84Db FBgn0004173 = CG17934|FBan0017934|CT39960| *F (There happens to be an ORF on the opposite strand: CG14599. Do not merge. *F The translation of CG17934 matches that of Mst84Db annotated in Acc. X67703) *F *F I think there is a bit of a mixup between LIP1 and Lip1, *F they both have the same accession (Y14366). That accession *F belongs to Lip1 (Lipase 1), I don't think there is an accession *F associated with LIP1 (LAR-interacting protein 1). *F ... *F Lip1 FBgn0023496=CG7279|FBan0007279|CT22413|FBan0007279 *F (CG7279 is now wrongly annotated as LIP1 FBgn0024747) *F *F HDAC3 FBgn0025825 438 aa = CG2128|FBan0002128|CT6896|438 aa *F (Aubrey: Protein ID is missing from gene report: AAC83649.1 *F This was missing from the CDS set) *F *F mit(1)15 FBgn0004643 = CG9900|FBan0009900|CT25082| # *U FBrf0126924 *a Misra *b S. *t 2000.4.12 *T personal communication to FlyBase *u *F Category 12: genes located way away from their published cytology. *F Could be (a) mis-assignment of CG to FBgn, (b) community bloopers *F or (c) gross assembly errors. I could only find three cases where *F a mis-assignment seemed very clear. (Note that I excluded cases *F that have already been discussed/sorted, namely Tak1, Ocr/Oamb and *F Hrb87F/85DE.) *F *F Sima analyzed by ClustalW of protein sequences from aa_embl.dros (except *F RpS9 protein from NCBI, fragileX nucleotide sequence from NCBI) and *F aa-gadfly.dros, to rule out mis-assignment. Then analyzed community *F nucleotide sequence for gene f rom GenBank by BLASTN at NCBI against *F Drosophila genome to check matches to scaf fold. Looked at nearby genes *F on scaffold to see if these localizations made sen se, but didn't have a *F way to really distinguish community mistakes from assembly errors. For *F Fragile X related, BLASTed at BDGP against na_gadfly.dros to find correct *F assignment. *F *F from Leyla: *F *F >CG4265/Uch 22D3 from genome sequence but community says 98F *F matches gb|AE003584.1|AE003584 Drosophila melanogaster genomic scaffold *F 142000013386046 ~14721-14574, 17052-18539 *F *F from Sima: *F *F >emb|AF137270|AF137270 inx6 FBgn0027107 AF137270:163..1479(438aa) *F =CG2977|FBan0002977|CT9644|FBan0002977 last_updated:000321(438aa) *F matches AE003439 Drosophila melanogaster genomic scaffold 142000013386054 *F section 23 of 35, ~151333-150929, 150635-149925, 149854-149366 *F cm at 6E7, inx2 at 6E4-5 *F >emb|AF137271|AF137271 inx7 FBgn0027106 AF137271:106..612(169aa) *F =CG17063|FBan0017063|CT35571|FBan0017063 last_updated:000321(481aa) *F significant N-terminal addition and short C-terminal addition as well *F matches AE003572 Drosophila melanogaster genomic scaffold 142000013386037 *F section 5 of 5, ~41005-41616 *F Rab10 at 19C4, Peritrophin-A at 19C4 *F CG2977/inx6 and CG17063/inx7 are in each other's community locations (6E, *F 19C) *F *F >emb|D17389|DMRYRH_2 Rya-r44F FBgn0011286 SPTREMBL:Q24498 D17389(5126aa) *F =CG10844|FBan0010844|CT30357|FBan0010844 last_updated:000321(5107aa) *F >emb|Z18536|DMDRYA Rya-r76CD FBgn0010268 SPTREMBL:Q24321 *F Z18536:2..1528(509aa) *F matches AE003835 Drosophila melanogaster genomic scaffold 142000013386047 *F section 45 of 52, ~193616-193057, 193039-191190, 191184-189835, 189792-181015, *F 180998-177611, 177552-177389, 177328-174689, 174671-173654, 173637-172177, *F 172105-167929 *F =CG10844|FBan0010844|CT30357|FBan0010844 last_updated:000321(5107aa) *F Z18536 missing first ~4618aa, has different last ~30aa, but otherwise same *F matches AE003835 Drosophila melanogaster genomic scaffold 142000013386047 *F section 45 of 52, ~169947-168416 *F Pgi at 44F7, Ggamma1 at 44F6 *F Rya-r76CD looks like Rya-r44F *F *F >emb|AF080266|AF080266 Sptr FBgn0014032 AF080266:42..827(261aa) *F =CG12117|FBan0012117|CT7144|FBan0012117 last_updated:000321(261aa) *F matches AE003444 Drosophila melanogaster genomic scaffold 142000013386054 *F section 28 of 35, ~48658-47758 *F org-1 at 7E6, Es2 at 7E6, Cp36 at 7E7 *F CG12117/Sptr 7E6 from genome sequence but community says 15A *F *F >emb|AB023511|AB023511 6-phosphofructo-2-kinase FBgn0027621 *F AB023511:403..2553(716aa) *F =CG3400|FBan0003400|CT11373|FBan0003400 last_updated:000321(717aa) *F matches AE003512 Drosophila melanogaster genomic scaffold 142000013386053 *F section 29 of 30, ~100293-100443, 100487-100802, 108405-110824 *F pcm at 18D3, Grip84 at 18D8 *F CG3400/6-phosphofructo-2-kinase 18D3 from genome but community says *F 17C--D *F *F >emb|AF006639|AF006639 Ser4 FBgn0020906 SPTREMBL:O16101 *F AF006639:232..1029(265aa) *F =CG8867|FBan0008867|CT25434|FBan0008867 last_updated:000321(266aa) *F matches AE003608 Drosophila melanogaster genomic scaffold 142000013386055 *F section 1 of 63, ~14881-14438, 14424-13601, 12510-12454, 10015-9950, 9931-9810 *F betaggt-I at 25C1, Trip1 at 25C1 *F CG8867/Ser4 25C1 from genome sequence but community says 56E3 *F *F >emb|X77926|DMBBBC1_2 RpL13 FBgn0011272 SWISS-PROT:P41126 X77926(218aa) *F =CG4651|FBan0004651|CT15029|FBan0004651 last_updated:000321(218aa) *F matches AE003626 Drosophila melanogaster genomic scaffold 142000013386055 *F section 19 of 63, ~256361-256379, 256636-257512 *F Cyp4e3 at 30C9, sop at 30E1, Dref at 30E4 *F CG4651/RpL13 30E4 from genome sequence but community says 29E--F *F *F >emb|X15497|DMPROS35_2 Pros35 FBgn0003151 SWISS-PROT:P12881 *F X15497:71..910(279aa) *F =CG4904|FBan0004904|CT15762|FBan0004904 last_updated:000321(279aa) *F matches AE003627 Drosophila melanogaster genomic scaffold 142000013386055 *F section 20 of 63, ~245241-245264, 245332-245963, 246021-246315 *F Sur at 31A2, RpL7 at 31A2, NPC1 at 31A3 *F CG4904/Pros35 31A3 from genome sequence but community says 89F--90A *F *F >emb|U70979|U70979 slv FBgn0025469 U70979:283..963(226aa) *F =CG8717|FBan0008717|CT3863|FBan0008717 last_updated:000321(226aa) *F matches AE003838 Drosophila melanogaster genomic scaffold 142000013386047 *F section 48 of 52, ~225463-225818, 225894-225973, 226330-226560, 226624-226802, *F 226871-227274 *F sut1 at 44A4 *F CG8717/slv 44A4 from genome sequence but community says 76A--B *F *F >emb|D17551|DMDPR2 PR2 FBgn0013955 SPTREMBL:Q24316 D17551:1..2300(765aa) *F =CG3969|FBan0003969|CT13197|FBan0003969 last_updated:000321(1238aa) *F D17551 has extra 70aa at N-terminus, CG3969 missing ~436-462aa, has extra *F C-terminus from 766-1238aa *F matches AE003819 Drosophila melanogaster genomic scaffold 142000013386047 *F section 29 of 52, ~181638-181677, 181756-182155, 182211-183088, 183038-183294, *F 183361-184093, 184151-184373, 188353-188600 *F Mp20 at 49F15, Dp at 49F11-13, lat at 49F10, Nrk at 49F7, TppII at 49F7, *F CG3969/PR2 49F6 from genome sequence but community says 31F1--2 *F *F >emb|Y07720|DMNADCOTR_2 NaPi-T FBgn0016684 SPTREMBL:Q94886 *F Y07720:439..2013(524aa) *F =CG10207|FBan0010207|CT28711|FBan0010207 last_updated:000321(524aa) *F matches AE003813 Drosophila melanogaster genomic scaffold 142000013386047 *F section 23 of 52, ~48414-48860, 48990-49206, 49323-49569, 49622-50075, *F 50134-50268, 50339-50580, 50645-51573 *F Ercc1 at 51D2, SMC2 at 51D2, BEAF-32 at 51C4 *F CG10207/NaPi-T 51D2 from genome sequence but community says 43B--C *F *F >gi|6503205|gb|AF205596.1|AF205596 Drosophila melanogaster clone LD09557 *F Fragile X related mRNA, complete cds(3250bp) *F =CG6203|FBan0006203|CT17036|FBan0006203 last_updated:000321(2455bp) *F not CG6727! *F matches AE003685 Drosophila melanogaster genomic scaffold 142000013386035 *F section 10 of 105, ~173318-173164, 172260-172043, 169463-169139, 169073-168906, *F 168719-168418, 168361-168227, 168147-167638, 166951-166753, 166693-166432, *F 166340-166221, 166169-165817, etc. *F CG6203 at 85F11 *F CG6727/Fragile-X-related 58D1 from genome sequence but community says *F 85F12 *F *F >emb|U65105|DMU65105_2 Reg-5 FBgn0015801 SPTREMBL:Q94913 *F U65105:538..1434(298aa) *F =CG2928|FBan0002928|CT9939|FBan0002928 last_updated:000321(288aa) *F disagree at C-termimus from 236aa on *F matches AE003465 Drosophila melanogaster genomic scaffold 142000013386038 *F section 14 of 15, ~96933-96960, 102290-101678, 98057-97866, 97737-96895 (messed *F up assembly?) *F Orc4 at 60D12, Dll at 60D13--14 *F CGC2928/Reg-5 60D12 from genome sequence but community says 100C *F *F >emb|U34329|DM34329 Cyp4d8 FBgn0015033 SPTREMBL:Q24127 *F U34329:2..385(128aa) *F =CG4321|FBan0004321|CT14135|FBan0004321 last_updated:000321(515aa) *F CG4321 has extra 314aa at N terminus and extra 65aa at C terminus *F matches AE003558 Drosophila melanogaster genomic scaffold 142000013386050 *F section 45 of 54, ~258714-258940, 259001-259158 *F lqf at 66A4--5 *F CG4321/Cyp4d8 66A2 from genome sequence but community says 2E *F *F >gi|4688682|emb|CAB41492.1| ribosomal protein [Drosophila *F melanogaster](197aa) *F =CG3395|FBan0003395|CT11393|FBan0003395 last_updated:000321(195aa) *F only 65% match *F matches AE003551 Drosophila melanogaster genomic scaffold 142000013386050 *F section 38 of 54, ~251646-251897, 252308-252474, 252793-253057 *F LanB2 at 67B5--6 *F CG3395/RpS9 67B2 from genome sequence but community says 69 *F *F >emb|U18973|DM18973_2 Pdi FBgn0014002 SWISS-PROT:P54399 *F U18973:10..150(496aa) *F =CG6988|FBan0006988|CT21638|FBan0006988 last_updated:000321(496aa) *F matches 75160-75318, 78094-79886 *F ind at 71A4 *F CG6988/Pdi 71B2 from genome sequence but community says 74 *F *F >emb|AF154418|AF154418 fau FBgn0020439 AF154418:95..490(131aa) *F =CG6544|FBan0006544|CT41976|FBan0006544 last_updated:000321(163aa) *F only share first 25aa *F =CG6544|FBan0006544|CT41978|FBan0006544 last_updated:000321(217aa) *F only share first 25aa, shares first 35aa with CT41980 *F =CG6544|FBan0006544|CT41980|FBan0006544 last_updated:000321(98aa) *F only share first 25aa *F matches AE003688 Drosophila melanogaster genomic scaffold 142000013386035 *F section 13 of 105, 132431-132261, 129103-128697, 128674-128632, 128613-128423 *F Takr86C at 86C9, TfIIFbeta at 86C10, Rbp1 at 86C12 *F CG6544/fau 86C11 from genome sequence but community says 7C--D *F *F >emb|X73134|DMGCR20_2 anon-Pen50DE FBgn0003060 SPTREMBL:Q24347 *F X73134(127aa) *F =CG9757|FBan0009757|CT27573|FBan0009757 last_updated:000321(127aa) *F matches AE003701 Drosophila melanogaster genomic scaffold 142000013386035 *F section 26 of 105, ~11886-11323 *F sqd at 87F9 *F CG9757/anon-Pen50DE 87F7 from genome sequence but community says 50D--E *F *F >emb|AF132535|AF132535 ldlCp FBgn0026634 AF132532(710aa) *F =CG6177|FBan0006177|CT19390|FBan0006177 last_updated:000321(710aa) *F matches AE003708 Drosophila melanogaster genomic scaffold 142000013386035 *F section 33 of 105, 162093-161811 *F Surf4 at 88F4 *F CG6177/ldlCp 88F4 from genome sequence but community says 36C *F *F >emb|M71250|DMEDG91A_3 Edg91 FBgn0004554 SWISS-PROT:P27781 M71250(159aa) *F =CG7539|FBan0007539|CT23087|FBan0007539 last_updated:000321(159aa) *F matches AE003718 Drosophila melanogaster genomic scaffold 142000013386035 *F section 43 of 105, ~173019-172592, 146376-144875 BUT first 458 bp of M71250 *F repeated many times in genome *F Sgs5 at 90B3, osa at 90B7 *F CG7539/Edg91 90B3 from genome sequence but community says 91A *F *F >emb|AF061507|AF061507 PyK FBgn0003178 SPTREMBL:O62619 *F AF061507:198..1799(533aa) *F =CG7070|FBan0007070|CT21861|FBan0007070 last_updated:000321(533aa) *F matches AE003738 Drosophila melanogaster genomic scaffold 142000013386035 *F section 63 of 105, ~174023-174259, 175470-176861, 176919-177076, 177163-177428 *F CG7070/PyK 94A14 from genome sequence but community says 11E--12A *F *F >emb|Y12400|DMORCT2_2 Orct FBgn0019952 Y12400:310..1956(548aa) *F =CG6331|FBan0006331|CT19810|FBan0006331 last_updated:000321(548aa) *F matches AE003747 Drosophila melanogaster genomic scaffold 142000013386035 *F section 72 of 105, ~27398-29458 *F jar at 95F5, nAcRalpha-96Aa at 96A1 *F CG6331/Orct 95F6 from genome sequence but community says 29D--E *F *F >emb|Z33647|DMMACGP_6 Mst57Db FBgn0011669 SPTREMBL:Q24391 *F Z33647:2149..2271(40aa) *F =CG5016|FBan0005016|CT16092|FBan0005016 last_updated:000321(40aa) *F matches AE003753 Drosophila melanogaster genomic scaffold 142000013386035 *F section 78 of 105, ~180457-180127, 171000-169973, 169992-167604 *F Mst57Dc at 96F3 *F CG5016/Mst57Db 96F3 from genome sequence but community says 57D *F *F >emb|AF142325|AF142325 Mst57Dc FBgn0011670 AF142325:210..518(103aa) *F =CG4986|FBan0004986|CT15987|FBan0004986 last_updated:000321(103aa) *F matches AE003753 Drosophila melanogaster genomic scaffold 142000013386035 *F section 78 of 105, ~168229-167711 *F MstDb at 96F3 *F CG4986/Mst57Dc 96F3 from genome sequence but community says 57D *F *F >emb|Z33647|DMMACGP_5 Mst57Da FBgn0011668 SPTREMBL:Q24390 *F Z33647:1204..1371(55aa) *F =CG9074|FBan0009074|CT26012|FBan0009074 last_updated:000321(75aa) *F CG9074 has extra 20aa at C-terminus *F matches AE003753 Drosophila melanogaster genomic scaffold 142000013386035 *F section 78 of 105, ~180457-180127, 171000-169973, 169992-167604 *F Mst57Dc at 96F3, MstDb at 96F3 *F CG9074/Mst57Da doesn't yet have a CG or genome cyto but was in Category *F 10 *F *F >emb|L27654|DMPGLY_4 Pglym78 FBgn0014869 SPTREMBL:Q24450 L27654(192aa) *F =CG1721|FBan0001721|CT4904|FBan0001721 last_updated:000321(199aa) *F CG1721 has extra 7aa at N-terminus *F matches AE003768 Drosophila melanogaster genomic scaffold 142000013386035 *F section 93 of 105, ~115128-117252 *F yemalpha at 99A3, stg at 99A7 *F CG1721/Pglym78 99A4 from genome sequence but community says 78A--B *F *F >emb|AF005843|AF005843 anon-EST:fe1A3 FBgn0022349 SPTREMBL:O16040 *F AF005843(341aa) *F =CG1910|FBan0001910|CT5892|FBan0001910 last_updated:000321(489aa) *F AF005843 missing first ~147aa at N-terminus *F matches AE003779 Drosophila melanogaster genomic scaffold 142000013386035 *F section 104 of 105, ~159116-160079, 160135-160497 *F awd at 100E2, anon-100EF-D3 at 100E3 *F CG1910/anon-EST:fe1A3 100E2 from genome sequence but community says 71A # *U FBrf0126925 *a Dunkov *b B. *t 2000.6.29 *T personal communication to FlyBase *u *F Table of the D. melanogaster cytochrome P450 genes, their *F cytological locations and annotated Celera transcripts (CTs). *F *F Cytological locations of the P450 genes were determined from the known *F location of adjacent genes. When available, the localization data for *F individual P450 genes obtained by in situ hybridization were taken into *F account. A single location is given for clustered genes. Inversely, *F genes (or gene clusters) listed separately with the same location are *F not clustered. Only tandem groupings of two or more P450 genes in any *F orientation, not separated by other genes, were considered as clusters. *F All genes, including the individual genes in a cluster, are listed in *F the order of increasing cytology. *F *F cytology , gene , CT *F unmapped , Cyp307a2p , none *F 1B , Cyp4g1 , 13187 *F 2D6-E1 , Cyp4ae1 , 30144 , *F 2D6-E1 , Cyp4d1 , 12233, 12253 *F 2D6-E1 , Cyp4d14 , 11900 *F 2D6-E1 , Cyp4d2 , 11675 , *F 10B8-C2 , Cyp4g15 , 36755 *F 11A , Cyp28c1 , 5856 , *F 11A , Cyp311a1 , 3715 *F 11A , Cyp318a1 , 31700 *F 13A2-C1 , Cyp4s3 , 26062 *F 17C , Cyp18a1 , 20826 *F 17C , Cyp306a1 , 20454 *F 17C , Cyp308a1 , 20468 *F 19E2-3 , Cyp6v1 , 5576 *F 19F4 , Cyp6t1 , 4488 *F 22F , Cyp309a1 , 28097 *F 22F , Cyp309a2 , 10919 *F 25D6 , Cyp28d1 , 30336 *F 25D6 , Cyp28d2 , 17788 *F 25D6 , Cyp4ac1 , 33591 *F 25D6 , Cyp4ac2 , 40069 *F 25D6 , Cyp4ac3 , 33590 *F 25F1 , Cyp6a16 , 22353 *F 28B1-2 , Cyp4d21 , 20878 *F 30C5-7 , Cyp4e3 , 13600 *F 34E4-5 , Cyp28a5 , 25460 *F 35F6-12 , Cyp303a1 , 13756 *F 36F , Cyp310a1 , 29164 *F 39C1-3 , Cyp6t2p , none *F 42A , Cyp6w1 , 4868 *F 42D , Cyp6a2 , 11585 *F 42E , Cyp6u1 , 11986 *F 43A1-2 , Cyp9b1 , 14604 *F 43A1-2 , Cyp9b2 , 14594 *F 44D , , Cyp6a13 , 7990 *F 44D , Cyp4ad1 , 6880 *F 44D , Cyp4e1 , 6692 *F 44D , Cyp4e2 , 6686 *F 44D , Cyp6a14 , 6361 *F 44D , Cyp6a15p , 34554 *F 45C , Cyp4p1 , 30367 *F 45C , Cyp4p2 , 6078 *F 45C , Cyp4p3 , 30369 *F 47A , Cyp49a1 , 32039 *F 47E , Cyp12d1 , 26082 *F 48E12-F , Cyp6g2 , 25452 *F 48E12-F , Cyp6t3 , 24763 *F 48E12-F1 , Cyp6g1 , 24751 *F 49B1-2 , Cyp301a1 , 24987 *F 49D , Cyp9h1 , 33386 *F 51C , Cyp317a1 , 31993 , *F 51C , Cyp6a17 , 28783 , *F 51C , Cyp6a19 , 28789 , *F 51C , Cyp6a20 , 28791 , *F 51C , Cyp6a21 , 28795 , *F 51C , Cyp6a22 , 28781 *F 51C , Cyp6a23 , 28787 , *F 51C , Cyp6a8 , 28799 , *F 51C , Cyp6a9 , 28793 , *F 52D2 , Cyp4aa1 , 20080 , *F 55F1-2 , Cyp12b2 , 34952 , *F 58E , Cyp6d2 , 14177 , *F 60D13-16 , Cyp9c1 , 12139 *F 62E , Cyp4d20 , 8030 *F 64A5 , Cyp302a1 , 2045 *F 64E , Cyp307a1 , 29702 *F 66A5 , Cyp316a1 , 15730 *F 66A5 , Cyp4d8 , 14135 *F 70E4-5 , Cyp314a1 , 32843 , *F 75C1-2 , Cyp312a1 , 16459 *F 75D4-5 , Cyp12c1 , 13666 *F 76B4 , Cyp305a1 , 25219 *F 85A1-10 , Cyp313b1 , 27472 *F 86B6-C1 , Cyp12e1 , 34465 *F 86F-87A1 , Cyp315a1 , 34521 *F 87B-C , Cyp9f2 , 17908 *F 87B-C , Cyp9f3p , 39772 *F 87B4 , , Cyp313a3 , 28411 *F 87B4 , Cyp313a2 , 28413 *F 87B4 , Cyp313a5 , 28421 *F 87C , Cyp313a4 , 21009 *F 87D , Cyp304a1 , 22337 *F 88A-B , Cyp6d5 , 10252 *F 88C , Cyp313a1 , 11271 *F 91F1-13 , Cyp12a4 , 18860 *F 91F1-13 , Cyp12a5 , 33912 *F 94C , Cyp6d4 , 38889 *F 98A , Cyp6a18 , 33531 *F 100B1-2 , Cyp4c3 , 3417 # *U FBrf0126926 *a Bayraktaroglu *b L. *t 2000.4.18 *T personal communication to FlyBase *u *F mod(mdg4) all 3 adjacent but CG15802 on opposite strand *F *F This is because part of the Celera sequence is in the wrong orientation *F WRT surrounding sequence. *F *F Technically, this is a merge, but do we want to merge CGs on *F opposite strands? *F *F > mod(mdg4) FBgn0002781 = CG15802|FBan0015802|CT40910|CT20301|CT40908| *F AND CG7836|FBan0007836|CT23629| *F AND CG8076|FBan0008076|CT23762| *F The problem is in the region around 136941-138566 of AE003734 (these are *F approximate coordinates based on cDNA alignment, I am not sure where the *F exact flip is). I think it is the Celera sequence that's wrong and not the *F cDNAs in GenBank because the cDNAs from different labs agree with one another. # *U FBrf0126927 *a Nelson *b D.R. *t 2000.4.17 *T personal communication to FlyBase *u *F >From dnelson@utmem.edu Mon Apr 17 14:21:19 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 14:21:19 +0100 *F Date: Mon, 17 Apr 2000 08:25:58 -0500 *F From: David Nelson *F Subject: Re: Drosophila CytP450's & FlyBase *F To: 'Michael Ashburner (Genetics)' *F MIME-version: 1.0 *F X-Mailer: Microsoft Outlook Express Macintosh Edition - 4.5 (0410) *F Content-transfer-encoding: 7bit *F X-Priority: 3 *F *F Dear Michael, *F *F 6t2p has three in frame stops that make it a pseudogene compared to its *F close cousins 6t1 and 6t3 shown below *F *F 6t1 AC012831 comp(42975-44564) no introns *F MIAVFSLIAAALAVGSLVLLPVVLRGGCLLVVTIVWLWQILHFWHWRRLGVPFVPAAPFV *F GNVWNLLRGACCFGDQFRELYESKEAAGRAFVGIDVLHNHALLLRDPALIKRIMVEDFAQ *F FSSRFETTDPTCDTMGSQNLFFSKYETWRETHKIFAPFFAAGKVRNMYGLLENIGQKLEE *F HMEQKLSGRDSMELEVKQLCALFTTDIIASLAFGIEAHSLQNPEAEFRRMCIEVNDPRPK *F RLLHLFTMFFFPRLSHRVGTHLYSEEYERFMRKSMDYVLSQRAESGENRHDLIDIFLQLK *F RTEPAESIIHRPDFFAAQAAFLLLAGFDTSSSTITFALYELAKNTTIQDRLRTELRAALQ *F SSQDRQLSCDTVTGLVYLRQVVDEVLRLYPPTAFLDRCCNSRTGYDLSPWNGGSPFKLRA *F GTPVYISVLGIHRDAQYWPNPEVFDPERFSAEQRQQHHPMTYLPFGAGPRGCIGTLLGQL *F EIKVGLLHILNHFRVEVCERTLPEMRFDPKAFVLTAHNGTYLRFVKNSL *F *F 6t2p AC014742 1042-2504 *F MVIAFFIFL*CAALAVGSVVLLPLIALLAVWLWQRRHFRIWRRLGVPYLPAAPVLGNVLN *F VETAACCFGDQFRELYERKEAAGRAIVGINVLHSHALLLRDPALIRRILVEDFPEFSSSF *F KSTDAIRDTMGSGNLLFTKYKTWWETHKIFAIRLGGRRIRSLLYGLLERI*QNLEAHMAQ *F KLNGAESVELEVKQLCALFTTDIFAKFALQSLQNPEAEFRPMCIEVNDPKPKRLSHHLFT *F GFTPPIYRVRTHLYSEEYERFMRKSMNYVLAQRAEN*EKRYDLIDMFLQMHRTETAEGII *F HRPDFYVAQAAFLLLAGFDTSSSFALYELAKNPTIEHRLQAELRVDLQSSHNHQLSYDTL *F TGLVYLRQVLEDLPFGAGPRGCIGTLLGQLGIKVGLLHTLKHFRVELCERTLPEMRFDP* *F ASVLTAHNGTFLRFVRNSL* *F *F 6t3 AC015208 comp(17123-18689) *F MLLIWLLLLTIVTLNFWLRHKYDYFRSRGIPHLPPSSWSPMGNLGQLLFLRISFGDLFRQ *F LYADPRNGQAKIVGFFIFQTPALMVRDPELIRQVLIKNFNNFLNRFESADAGDPMGALTL *F PLAKYHHWKESRQCMSQLFTSGRMRDVMYSQMLDVASDLEQYLNRKLGDRLERVLPLGRM *F CQLYTTDVTGNLFYSLNVGGLRRGRSELITKTKELFNTNPRKVLDFMSVFFLPKWTGVLK *F PKVFTEDYARYMRHLVDDHHEPTKGDLINQLQHFQLSRSSNHYSQHPDFVASQAGIILLA *F GFETSSALMGFTLYELAKAPDIQERLRSELREAFISTATLSYDTLMTLPYLKMVCLEALR *F LYPAAAFVNRECTSSASEGFSLQPHVDFIVPPGMPAYISILGLHRDERFWPEPCVFDPER *F FGPERSRHIHPMTYIPFGAGPHGCIGSRLGVLQLKLGIVHILKQYWVETCERTVSEIRFN *F PKSFMLESENEIYLRFCRSSL *F *F 6a15p is short and has stops and frameshifts. Compare to 6a16 below *F *F 6a15p this is a pseudogene on AC020451 2595-3373 with in frame stops and *F frame shifts *F AFTVTNSKLAKKLKMKILRDDLTDFFLSVVKPALSGMTLWTSPPSGGRSSRQGGSKFDLS *F HNWTLEQMAAQAIVFFLAGFETSSSTMSSCKYELALQPEI*NQIRDEIERVLEGNAITYD *F ALAKINYPEQVLSETLRKHPIQLIKFLLETQESFRVRNTELIVEKGTSLLIPVHSVHYDP *F HLYPHPKLFDSSRLKAYKSNSRHPFAYLPFGTFGPRSCIGLRFGKMQAKIGIVSLCQRFK *F FGDSDLTDIPL *F *F 6a16 AC004721 comp(2956-5070) *F MDFTLLLLTSLLSFLLGYLRYRFTYWELRGIPQLRPHFLFGHFFRLQSVHYSELLQETYD *F AFRGSAKVAGTYVFLRPMAVVLDLDLVKAVLIRDFNNFVDRRSFHGDPLTANLFNLQGEE *F WRNLRTKLSPTFTSGKMKYMFGTVSTVAQQLGGTFDELVGSQGAVLELHDLMARYTTDVI *F GSCAFGTECSSLREPQAEFRQVGRRIFRNSNRSIRWRIFKMTYLSSLAKLGLPVRILHPD *F ITKFFNRIVRETVELRERENIRRNDFMDLLLDLRRKGLTMEQMAAQAFVFFVAGFETSSS *F NMSYALFELAKNQDVQQKLRMEINDSIGKHGKLTYEAMMEMPYLDQTITETLRKYPALSS *F LTRLASEDYEIPSPDGGDPVVLEKGTSVHIPVLAIHYDPEVYPEPHEFRPERFAPDACRE *F RHPTAFLGFGDGPRNCIGLRFGRMQVKVGLITLLRRFRFSLPPGSPTQLKVTKRNLILLP *F SDGVRLQVDPVESRLM *F *F 307a2p is a short N-terminal fragment of a P450. It is missing nearly all *F of the seq. compare to 307a1. *F *F 307a1 AC014810 comp(54284-52654) also AC007840 *F MLAALIYTILAILLSVLATSYICIIYGVKRRVLQPVKTKNSTEINHNAYQKYTQAPGPRP *F WPIIGNLHLLDRYRDSPFAGFTALAQQYGDIYSLTFGHTRCLVVNNLELIREVLNQNGKV *F MSGRPDFIRYHKLFGGERSNSLALCDWSQLQQKRRNLARHCCEEMEHWNRELGNQLVPGE *F PINIKPLILKACANMFSQYMCSLRFDYDDVDFQQIVQYFDEIFWEINQGHPLDFLPWLYP *F FYQRHLNKIINWSSTIRGFIMERIIRHRELSVDLDEPDRDFTDALLKSLLEDKDVSRNTI *F IFMLEDFIGGHSAVGNLVMLVLAYIAKNVDIGRRIQEEIDAIIEEENRSINLLDMNAMPY *F TMATIFEVLRYSSSPIVPHVATEDTVISGYGVTKGTIVFINNYVLNTSEKFWVNPKEFNP *F LRFLEPDNEKLQLKRNIPHFLPFSIGKRTCIGQNLVRGFGFLVVVNVMQRYNISSHNPST *F IKISPESLALPADCFPLVLTPREKIGPL *F *F 307a2p AC019383 comp(3284-3706) AL078186 *F MLTSVFYVLFAIAITIILISYVFLLLKCKQKAFVVIGLLYQEKKYQCFDQAPGPHPWPII *F GNINLLGRFQYNPFYGFGTLTKKYGDIYSLSLGHTRCIVVNNVDLIKEVLNKNGKYFGGR *F PDFFRYHKLFGGDRNNCKFIXXLRF *F *F 9f3p is missing an exon or two at the active site. This includes the heme *F binding cys that is essential for activity. *F *F 9f2 AC017132 AC017240 AC009741 AC017170 AC007594 PLUS ESTs *F MLWEFFALFAIAAALFYRWASANNDFFKDRGIAYEKPVLYFGNMAGMFLRKRAMFDIVCD *F LYTKGGSKKFFGIFEQRQPLLMVRDPDLIKQITIKDFDHFINHRNVFATSSDDDPHDMSN *F LFGSSLFSMRDARWKDMRSTLSPAFTGSKMRQMFQLMNQVAKEAVDCLKQDDSRVQENEL *F DMKDYCTRFTNDVIASTAFGLQVNSFKDRENTFYQMGKKLTTFTFLQNMKFILLFALKSL *F NKILKVEIFDRKSTQYFVRLVLDAMKYRQEHNIVRPDMINMLMEARGIIQTEKTKASAVR *F EWSDRDIVAQCFVFFFAGFETSAVLMCFTAHELMENQDVQQRLYEEVQQVDQDLEGKELT *F YEAIMGMKYLDQVVNEVLRKWPAAIAVDRECNK DITFDVDGQKVEVKKGDVIWLPTCGFH *F RDPKYFENPMKFDPERFSDENKESIQPFTYFPFGLGQRNCIGSRFALL EAKAVIYYLLKD *F YRFAPAKKSCIPLELITSGFQLSPKGGFWIKLVQRN *F *F 9f3p AC017132 AC017240 AC009741 AC017170 AC007594 PLUS ESTs *F MLWEFLALFAIATALFYRWASANNDFFKDRGIAYEKPVLYLGNMAGMFLRKRAMLDIVCD *F LYTKGGSGKFFGIFAQRQPLLMVRDPDLIKQITIKDFDHFINHRNEFDTSSDDDPHDMSN *F LFSSSLFSMRDARWKDMRSTLSPAFTGSKMRQMFQLMNQVAKEAVDCLKQDDSRVQENEL *F DMKDYCTRFTNDVIASTAFGLQVNSFKDRENTFYQMGKKLTTFTFLQSMKFMLFFALKGL *F NKILKVELFDRKSTQYFVRLVLDAMKYRQEHNIVRPDMINMLMEARGIIQTEKTKASAVR *F EWSDRDIVAQCFVFFFAGFETSAVLMCFTAHELMENQDVQQRLYEEVQQVDQDLEGKELK *F YLDQVVSEVLRKWPPAIAFDRECNK (see seq above for missing piece) *F EAKAVIYYLLKDYRFAPAKKSCIPLELISSGFQLS *F PKGGFWIKLVQRN *F *F By my definition of not producing a functioning P450 protein these are all *F pseudogenes. The def. of a psuedogene is a bit slippery. Some may say that *F these proteins are made into mRNA since some have ESTs and maybe into a *F protein, but it won't work as a P450. *F *F David *F *F ---------- *F >From: Michael Ashburner (Genetics) *F >To: ma11@gen.cam.ac.uk, dnelson@utmem.edu *F >Subject: Re: Drosophila CytP450's & FlyBase *F >Date: Sat, Apr 15, 2000, 8:29 AM *F > *F *F > David *F > *F > Three CytP450 gene symbols end in 'p'. Two of these have no CT. *F > Are these pseudogenes ? *F > *F > '87B-C','Cyp9f3p','39772' *F > '44D','Cyp6a15p','34554' *F > '39C1-3','Cyp6t2p','none' *F > 'unmapped','Cyp307a2p','none' *F > *F > Michael # *U FBrf0126928 *a Kennison *b J. *c J. *d Mueller *t 2000.4.25 *T personal communication to FlyBase *u *F >From kennisoj@dir6.nichd.nih.gov Mon Apr 24 18:48:59 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 24 Apr 2000 18:48:59 +0100 *F From: 'Kennison, James (NICHD)' *F To: 'Michael Ashburner' *F Subject: RE: Ch3d and Mi-2 - help FlyBase please *F Date: Mon, 24 Apr 2000 13:53:37 -0400 *F MIME-Version: 1.0 *F X-Mailer: Internet Mail Service (5.5.2651.58) *F *F Michael, *F *F CHD3 and Mi-2 are two different, but related genes. The map positions are *F correct, that is, CHD3 is in 76B and Mi-2 is in 76D. There are ESTs for *F both of these proteins, and the real CHD3 protein does appear to be *F significantly shorter than Mi-2 (Juerg Muller has sequenced enough of the *F CHD3 clones to verify that the real protein stops where the Celera predicted *F protein stops). There do not appear to be any problems with the genomic *F sequences for either of these two genes, although the annotated sequence for *F Mi-2 lacks the 5' published exon (it is about 20 kb more proximal than the *F other exons). *F *F Some of the confusion results from the CHD3 protein that was published by *F Woodage et al. (1997). This protein is a PCR artifact that fused the real *F amino terminus of CHD3 to the carboxy-terminus of Mi-2. Trevor Woodage is *F now at Celera, and he and I looked at these proteins in the genomic sequence *F during the annotation jamboree last November. He was going to contact *F Genbank about correcting the sequence last November, but I do not know *F whether he has done that yet. *F *F Jim Kennison *F ----------------------------------------------------------------------------- *F >From juerg.mueller@tuebingen.mpg.de Tue Apr 25 01:34:31 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 25 Apr 2000 01:34:31 +0100 *F X-Sender: juerg@mailer.tuebingen.mpg.de *F Mime-Version: 1.0 *F Date: Tue, 25 Apr 2000 02:37:54 +0200 *F To: Michael Ashburner (Genetics) *F From: Juerg Mueller *F Subject: Re: Ch3d and Mi-2 - help FlyBase please *F *F Dear Michael, *F *F thanks for the note. Yes, dMi-2 and CHD-3 are two different genes and *F there is a serious problem with the sequence published as AF007780. *F AF007780 is a hybrid sequence assembled from partial cDNAs and RT-PCR *F fragments (Woodage et al., PNAS 94, p. 11472). The first 2,4 kb of *F AF007780 are from a CHD3/CHD4 family member which we mapped to 76B whereas *F the 3' part of AF007780 is identical to 'our' dMi-2 which maps in 76D. *F dMi-2 is another CHD33/CHD4 family member. *F *F We isolated several dMi-2 cDNAs (Kehle et al., 1998), one of which is *F nearly fullength; we sequenced the genomic sequence of several dMi-2 *F alleles and there is no doubt that the published dMi-2 cDNA sequence is *F correct (AF119716). *F *F At the time we were puzzled by the AF007780 sequence and wondered whether *F it might be an alternatively spliced mRNA containing only the 3' portion of *F dMi-2. However, analysis of ESTs matching the AF007780 sequence made it *F clear that Woodage, the guy who published AF007780 and called it 'CHD-3' *F made an error. We partially sequenced a CHD3 EST (LD06032). The *F LD06032 sequence deviates from AF007780 at the same position where AF007780 *F starts to match the dMi-2 sequence. There is no doubt that Woodage patched *F together sequences from two different genes; i.e. CHD3 and dMi-2. *F *F I contacted Woodage at the time and mentioned that there is a problem with *F his sequence but he did not seem to bother to correct his entry in genbank. *F *F I hope that this helps to sort out the problem. Please let me know if you *F need more information. Is there a way to eliminate the AF007780 sequence *F from the databases? *F *F Best wishes, *F *F Juerg *F *F Juerg Mueller *F MPI fuer Entwicklungsbiologie *F Spemannstr. 35/III *F 72076 Tuebingen *F GERMANY *F phone: +49 7071 601491 *F fax: +49 7071 601384 *F *F *F ----------------------------------------------------------------------------- *F Dear colleague, *F *F I am sending this to those whose email addresses I can *F easily find. If necessary, please forward to the best person *F for an answer. *F *F *F There seems to be considerable confusion concerning the relationship between *F the Drosophila genes Mi-2 and Chd3. As authors on one or other *F of the relevant papers I enclose the analyses we have done with the *F available sequences from your labs and from Celera. *F *F I note that Mi-2 was said to map at 76D4 and Chd3 at 76B1. *F *F Any light you can throw on this problem would be greatly *F appreciated ! *F *F Our interpretation, which may be wrong, is that there has been an *F error in the assembly of AF007780 *F *F Thanks *F *F Michael Ashburner *F *F *F \**A are the EMBL records of these sequences; both are cDNAs. *F \**B is an alignment of these sequences. *F There is considerable overlap between these, and I suspect one or other groups has *F a cloning artifact, but cannot be sure. *F \**C are the protein sequences of these and of CG9594 & CG8103, the two related Celera genes. *F \**D is the same as \**C, but in fasta format *F \**E is a clustal of the Chd3 and Mi-2 proteins. *F they differ at N-term but are same at C-term, but the alignment is odd because over *F a long stretch there is considerable simialrity but far too many diffs for these to be *F due to polymorphisms. *F \**F is a clustal of the Mi-2 sequence and that of CG8103. *F Mi-2 is much longer than CG8103, but they are N-term identical, apart from slightly *F different methionine. *F \**G is a clustal of Chd3 sequence and that of CG8103. They are not similar. *F \**H is a clustal of Mi-2 sequence and that of CG9594. They are similar, but only 67%. *F \**I is a clustal of Chd3 sequence and that of CG9594. *F Mi-2 is much longer than CG9594, but they are N-term identical, apart from slightly *F different methionine. *F \**J is a clustal of all 4 proteins, with this tree: *F *F ( *F ( *F O97159:-0.22840, *F AAF49099.1:0.24084) *F :0.43090, *F O16102:-0.00965, *F AAF49162.1:0.07533); *F *F \**A *F ID AF119716 standard; RNA; INV; 6475 BP. *F XX *F AC AF119716; *F XX *F SV AF119716.1 *F XX *F DT 11-MAR-1999 (Rel. 59, Created) *F DT 03-MAR-2000 (Rel. 62, Last updated, Version 2) *F XX *F DE Drosophila melanogaster dMi-2 protein (dMi-2) mRNA, complete cds. *F XX *F KW . *F XX *F OS Drosophila melanogaster (fruit fly) *F OC Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; Pterygota; *F OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; *F OC Drosophilidae; Drosophila. *F XX *F RN [1] *F RP 1-6475 *F RX MEDLINE; 99055400. *F RA Kehle J., Beuchle D., Treuheit S., Christen B., Kennison J.A., Bienz M., *F RA Muller J.; *F RT 'dMi-2, a hunchback-interacting protein that functions in polycomb *F RT repression'; *F RL Science 282(5395):1897-1900(1998). *F XX *F RN [2] *F RP 1-6475 *F RA Mueller J.; *F RT ; *F RL Submitted (12-JAN-1999) to the EMBL/GenBank/DDBJ databases. *F RL MPI fuer Entwicklungsbiologie, Spemannstr. 35, Tuebingen 72076, Germany *F XX *F DR SPTREMBL; O97159; O97159. *F XX *F FH Key Location/Qualifiers *F FH *F FT source 1..6475 *F FT /chromosome='III' *F FT /db_xref='taxon:7227' *F FT /organism='Drosophila melanogaster' *F FT /map='76D' *F FT /clone_lib='Brown, N.H. and Kafatos, F.C., J.Mol.Biol. 203, *F FT p.425 (1988)' *F FT CDS 132..6080 *F FT /codon_start=1 *F FT /db_xref='SPTREMBL:O97159' *F FT /note='DNA-stimulated ATPase domain; chromodomains; *F FT PHD-finger motifs; similar to HMG-domain; similar to c-myb *F FT repeat; similar to the product encoded by GenBank Accession *F FT Number AF007780' *F FT /gene='dMi-2' *F FT /product='dMi-2 protein' *F FT /protein_id='AAD17276.1' *F FT /translation='MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDY *F FT DPEDSRRKKKGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRARKR *F FT KEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQ *F FT KENPKIAAPKLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSS *F FT RSSRNEKPDDIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRK *F FT RDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAP *F FT VVRKKAKTKIGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAY *F FT HLVCLEPELDEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSA *F FT YHTFCLNPPLDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSN *F FT SRVREYFIKWHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTR *F FT YKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWR *F FT ELPYDKSTWEEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDE *F FT DRPVKHYTPPPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTIL *F FT ADEMGLGKTIQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIG *F FT DKDSRAVIRENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAV *F FT LVVDEAHRLKSNQSKFFRILNSYTIAYKLLLTGTPLQNNLEELFHLLNFLSRDKFNDLQ *F FT AFQGEFADVSKEEQVKRLHEMLGPHMLRRLKTDVLKNMPSKSEFIVRVELSAMQKKFYK *F FT FILTKNYEALNSKSGGGSCSLINIMMDLKKCCNHPYLFPSAAEEATTAAGGLYEINSLT *F FT KAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKMLDILEDFLEGEQYKYERIDGGITGTLR *F FT QEAIDRFNAPGAQQFVFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIG *F FT QANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLVVRPGMGGKGANFTKQELDDILRFGT *F FT EDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEE *F FT ETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTT *F FT RDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARV *F FT GGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRH *F FT LCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKP *F FT CEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSE *F FT KEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKE *F FT ETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEKPSASSPKD *F FT QKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEKAAVPGREY *F FT EIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEIKNKFLARR *F FT FKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLSKESLAGNK *F FT PANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSRLAATAGNA *F FT SNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV' *F XX *F SQ Sequence 6475 BP; 1702 A; 1632 C; 1867 G; 1274 T; 0 other; *F gatatttgtt gaagccgctt acaattgact atttttttct cgtcgtgtct gtgtgtgctt 60 *F aattatttgt tgtggaaatt aagacaattt cgtgtgctca aacagtgtaa aagttgcatt 120 *F cgagtgctac aatggcatcg gaggaagaga atgacgataa tttccaggaa gaagaagagg 180 *F cccaggagga caacgcacct gcggcggagc tgtccaacga ttccgacgcc cctctgaagc 240 *F cgaataacga cgaagacgac gactacgatc cggaggacag tcgcaggaag aagaagggca 300 *F agaagcgcaa gaccaggaag ggcgaagaga agggtcgcaa gaagaagaag cgcaagaaga 360 *F acgagagcga agaggacagc gactttgtcc agcacgatga ggaggtggag taccccagca 420 *F cctccaagcg cgctcgcaag cgcaaggagg agaaacaggc cgccaaggag aaggagtcgg 480 *F catcatccgg aatgccatct gtggaagacg tgtgctcagc ctttagcgtc tgcaatgtgg 540 *F agatcgagta cagtgaggag gagctgcaga gcctaacgac ctacaaggct ttcatgcatc 600 *F atgttcgtcc gattctgcag aaggaaaacc ccaagattgc ggcaccaaag ctagtgatgc 660 *F tggtggcagc caagtggcgt gagttctgcg agagcaatcc gcacatccag caggagggtg 720 *F gtgccgccgg ctccggtgga tccgccggac aggcacgcag tgtgacaggc gatgagccgg 780 *F aggaacctcg atcatcgcgt tcctcgcgca acgagaaacc ggatgacatt tacgaggagg 840 *F ccgtcgagga ggaggaagaa gaggaggagg aggagaagaa acctcgtcgt aagcgcagtg 900 *F gacgtggcaa gaaggggcgt cgtccctccg gcaaggtgcc cactcttaag attaagctgc 960 *F tcggcaagcg caaacgcgat agttcggatg aggagcaaga tgctagcggt gcatcggaac 1020 *F gcgattcgga tcttgagttt gaacggatgc ttcagaaatc ggatgacagt gccgatgaaa 1080 *F aggaggctcc tgtctcctct aaggcggaca actcagcacc cgctgcccag gacgatggat 1140 *F caggagctcc agtggtgcgc aagaaggcca agaccaagat cggcaacaag ttcaagaaga 1200 *F agaataaact caagaaaacg aagaacttcc cggagggcga agatggcgag catgagcacc 1260 *F aggactactg cgaggtgtgc cagcaaggag gtgagatcat cctgtgcgac acctgccctc 1320 *F gggcatatca cttggtgtgc ctggagccag aactggatga accgccagag ggcaagtggt 1380 *F cgtgtccgca ctgcgaggct gacggaggtg ctgctgagga agaggacgat gatgagcacc 1440 *F aggaattctg ccgcgtgtgc aaggacggtg gcgagttgct gtgctgcgac tcatgtccct 1500 *F ccgcctatca cacattctgc ttgaatccgc ctctggacac tatacccgat ggcgattggc 1560 *F gctgtcctcg ttgcagctgc cctccgctca ctggcaaggc tgagaagatc atcacctggc 1620 *F gatgggcaca gcgtagcaat gacgacggtc cctccacctc gaagggatca aagaacagta 1680 *F actcccgggt gcgcgagtac tttatcaagt ggcacaacat gtcctactgg cactgcgaat 1740 *F gggtgcccga agtgcaactg gacgtccatc atccgctcat gattcgttcg ttccagcgca 1800 *F agtacgacat ggaagaaccg cccaagttcg aggagtcgct ggatgaggcg gacactcgtt 1860 *F acaagcgtat tcagcgacac aaggacaagg ttggcatgaa ggcaaacgat gatgccgaag 1920 *F tgctggagga gcggttctac aagaatggcg tcaagccaga gtggctcatt gttcagcgcg 1980 *F taatcaacca tcgtacggca agagatggca gcaccatgta tctggtgaag tggcgcgagt 2040 *F tgccctatga caagtccacc tgggaagaag agggtgatga catacagggc ctgcggcaag 2100 *F ccatcgatta ctatcaggat ctgcgtgcgg tttgcacctc ggagacaact cagtcgcgca 2160 *F gcaaaaagag caagaagggt cgcaagtcca agctcaaggt cgaggacgac gaggatcgcc 2220 *F cggtcaagca ctacacaccg ccgccggaaa agccaactac tgatctgaag aagaagtatg 2280 *F aggatcagcc ggctttcttg gagggcactg gcatgcaatt gcatccctac cagatcgagg 2340 *F gcattaactg gttgcgctac agctggggcc agggcatcga caccatcctg gccgacgaga 2400 *F tgggtctggg taagaccatt cagacggtca ccttcctgta ctctttgtac aaggagggcc 2460 *F attgccgcgg acccttcctc gtggccgtgc cactctcgac gctagtgaac tgggagcgtg 2520 *F agtttgagct gtgggctccg gacttttact gcatcacgta tatcggtgac aaggactcga 2580 *F gagcggttat acgggagaac gagctgagct tcgaggaggg cgccattcgt ggtagcaagg 2640 *F tttcgcgttt gcggaccacc cagtataaat tcaacgtact gctgactagc tacgagctga 2700 *F tctccatgga cgctgcttgc cttggcagca ttgactgggc cgtgctcgtg gtggacgagg 2760 *F cccatcgctt gaagagcaac cagagcaagt tcttccgtat cctgaacagc tacactattg 2820 *F cctacaagct tctgctcacc ggcacaccgc tgcagaacaa tctcgaggag ctgttccatc 2880 *F tgctcaactt cttgagccgc gacaagttca atgatctgca ggccttccag ggcgaattcg 2940 *F ctgacgtttc gaaggaggag caggttaagc gtctgcacga gatgcttgga ccgcacatgc 3000 *F tgcgtcgttt gaagacggat gtgctgaaga acatgccgtc caagtctgag tttatcgtgc 3060 *F gcgtcgagct gtcggccatg cagaaaaagt tctacaagtt catcttgacc aagaactacg 3120 *F aggctttgaa ttcgaagagc ggtggtggct cttgctcact gatcaacatt atgatggacc 3180 *F taaagaagtg ctgcaaccat ccgtatctct tcccctcggc cgccgaggag gcaaccaccg 3240 *F ctgccggtgg tctctacgag attaactcgc tgaccaaggc tgctggcaag ctggtcctgc 3300 *F tatccaagat gctgaagcag ctgaaggcac aaaaccatcg tgtcttgatt ttctcccaga 3360 *F tgaccaagat gttggacatt ctcgaggact tcctcgaggg cgagcagtac aagtacgaac 3420 *F gaattgacgg tggaatcaca ggaacattgc gtcaggaagc cattgatagg ttcaatgcac 3480 *F ccggagcgca gcagtttgtc ttcttgctga gtactcgagc cggaggattg ggtattaatt 3540 *F tggccacggc cgacactgtc attatttatg actccgattg gaatccccac aacgatattc 3600 *F aggccttctc ccgagcccat cgtattggcc aggctaacaa ggtgatgatc tatcgatttg 3660 *F ttactcgaaa ttctgtggag gagcgcgtta cccaggtggc caagcgtaag atgatgttga 3720 *F ctcatcttgt ggtccgtccg ggaatgggcg gtaaaggcgc taactttaca aagcaagaac 3780 *F tggacgatat ccttcgtttc ggtaccgagg atctgttcaa ggaggatgac aaggaggaag 3840 *F ctatccacta tgacgacaag gcggtggccg agctgctcga cagaaccaac cgcggtatcg 3900 *F aggagaaaga gtcctgggcc aacgagtatc tgtcctcgtt taaggtggct tcgtacgcca 3960 *F ctaaagaaga ggaggaggag gaagagacgg agattattaa acaggatgcc gagaactctg 4020 *F atccggcgta ctgggtgaag ctgctgcggc atcactatga acagcaccag gaagatgtgg 4080 *F gtcgcagcct gggcaagggc aagcgcgtcc gcaaacaggt caactacacg gatggcggcg 4140 *F ttgtggcggc tgataccacg cgggatgatt ccaactggca ggacaacggc agcgagtata 4200 *F actccgagta ttcagctggc tccgatgagg atggcggtga cgatgacttc gatgaccaga 4260 *F atggcgcaga gagaaaggcc aagcggcgat tggagcgtcg cgacgatcgt ccattgcctc 4320 *F cactgttggc ccgcgtgggt ggaaacatcg aggtgctcgg ttttaatgcc cgtcaacgca 4380 *F agagcttcct caatgccatt atgcggtacg gaatgccgcc gcaagatgcc ttcaactcgc 4440 *F aatggctagt ccgggatttg cgtggtaaat cggagcgcaa ttttaaggcg tatgtttctc 4500 *F tgtttatgcg gcatctttgc gagccgggag cggacaatgc ggaaaccttt gccgatggtg 4560 *F tgccgcgcga aggactatcc cgccagcatg tgctcacccg gatcggagtg atgtcgctaa 4620 *F ttcgaaagaa ggtacaggaa ttcgagcata ttaacggata ctacagcatg ccggagctca 4680 *F ttctcaagcc gtgcgagccc gtacgatcag ccctgaaaca ggatgtggcg gcacttgaag 4740 *F ctccgcctac cggtggcaat gtcgacaaga gcgctactac tagcaatagc gtcactccag 4800 *F ccaccagtgc ggctcccagt ccagcgccag cctctgaaaa aggtgaagac aaagacaagg 4860 *F actccgaaaa ggaaaaggat aagacctcgg cggagaagag cgaggtgaag caagaacaag 4920 *F aggctgaaga ggacaagaag cctggggatg tcaagcagga gaaccctgtg gaagaagctg 4980 *F ccggcgacac aaagcccagc gacgcggagg tcaaaaccga ggtggctaaa acagaaccca 5040 *F aggaagagac caaggacccg gaggtaaagg aggagcccaa gaccgaagag aaggaaaaag 5100 *F aaaaggtgga tgacaagaaa ccaataccac cgacgacagt aattgacgac gatgatgacg 5160 *F atgtgatgat tgtcaaggag gacggtgagc tagaaaagcc cagtgccagt tcacccaagg 5220 *F atcaaaaggc agtcgccgct gctacgtctg ctgcaactgg agccactggc aagggagcgg 5280 *F aggacagctt ggaggtgcta aagcgcaaat ttatgttcaa catcgccgac ggcgggttca 5340 *F ccgaactgca cactttgtgg ctcaacgagg agaaggctgc cgttcctggg cgagaatacg 5400 *F agatctggca ccgtcgccac gactactggc tgttggccgg aattgtgacc catggctatg 5460 *F gccgttggca ggatatacaa aacgatatcc gtttcgcgat cataaacgaa cctttcaaga 5520 *F tggacgttgg taagggcaac tttctggaaa tcaaaaacaa gttcctggcc cggcgtttta 5580 *F agttgctgga gcaggccctg gtcatcgagg agcagctgcg gcgagccgcc taccttaacc 5640 *F tcgcccaaga ccccagccac ccggcaatgt cgctaaacgc ccgtttcgca gaggtcgaat 5700 *F gcctggctga atcccatcag catctgagca aggaatcgct ggccggtaac aagcccgcaa 5760 *F acgctgtttt gcacaaggtg ctcaaccagc tcgaagagct gctctcggac atgaagagcg 5820 *F atgtgtcccg gctgccagct acactggctc gcatcccacc tgtggcgcaa cggcttcaga 5880 *F tgtccgagag atcgattctc tcccgactgg cggccaccgc cggaaatgcc tccaatgcag 5940 *F ctcaattgat ggcacaattc ccggctggat tccagggcac aacattgcca gcattcacga 6000 *F gcggaccggc tggcaacttt gccaactttc ggccacagtt ctcggtgccc ggccagctat 6060 *F cgaataattc cggcgtctag gcatctcctc aatatctatg ttaagagcat tcgtattcca 6120 *F tgcaaatata ctttactcaa gctaagaatt taaatttcta cacggtaaca cacccgcaca 6180 *F cccacacacc cacaacatat atttatcata atcatttgca atgaaatcca gatatcaaga 6240 *F aacaaaaaat ggacccacgt cgcgttcaca caaataatta atgcaaaacg tttgacactt 6300 *F ggcgaatggc gagttcggtg tttccaggct tcggtttctg ctcctggggc ggatcggatc 6360 *F tcagcagagc acgactcgcg gtcagttcac atacgacaag tacaatagtt cttattaact 6420 *F tctctactat gtgattacat aatctagata caaatataaa tgtatttaat tccat 6475 *F // *F // *F ID AF007780 standard; RNA; INV; 4912 BP. *F XX *F AC AF007780; *F XX *F SV AF007780.1 *F XX *F DT 27-NOV-1997 (Rel. 53, Created) *F DT 30-NOV-1997 (Rel. 53, Last updated, Version 2) *F XX *F DE Drosophila melanogaster CHD3 mRNA, partial cds. *F XX *F KW . *F XX *F OS Drosophila melanogaster (fruit fly) *F OC Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; Pterygota; *F OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; *F OC Drosophilidae; Drosophila. *F XX *F RN [1] *F RP 1-4912 *F RX MEDLINE; 97470991. *F RA Woodage T., Basrai M.A., Baxevanis A.D., Hieter P., Collins F.S.; *F RT 'Characterization of the CHD family of proteins'; *F RL Proc. Natl. Acad. Sci. U.S.A. 94(21):11472-11477(1997). *F XX *F RN [2] *F RP 1-4912 *F RA Woodage T.; *F RT ; *F RL Submitted (10-JUN-1997) to the EMBL/GenBank/DDBJ databases. *F RL Laboratory of Gene Transfer, National Human Genome Research Institute, *F RL National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892-4442, *F RL USA *F XX *F DR FLYBASE; FBgn0023395; Chd3. *F DR SPTREMBL; O16102; O16102. *F XX *F FH Key Location/Qualifiers *F FH *F FT source 1..4912 *F FT /db_xref='taxon:7227' *F FT /organism='Drosophila melanogaster' *F FT CDS <1..4558 *F FT /codon_start=2 *F FT /db_xref='FLYBASE:FBgn0023395' *F FT /db_xref='SPTREMBL:O16102' *F FT /gene='CHD3' *F FT /product='CHD3' *F FT /protein_id='AAB87384.1' *F FT /translation='SNSMSSKRGADPDWKTPGKASKDKRPKTNAKKQKFRDEEYCKVCS *F FT DGGDLLCCDSCPSVYHRTCLSPPLKSIPKGDWICPRCIPLPGKAEKILSWRWALDRSVE *F FT LRTSKGEKRREYFIKWHGMSYWHCEWIPEGQMLLHHASMVASFQRRSDMEEPSLEELDD *F FT QDGNLHERFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIP *F FT GLNQAIALYKKLRSSNKGRQRDRPAPTIDLNKKYEDQPVFLKEAGLKLHPFQIEGVSWL *F FT RYSWGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRGPFLISVPLSTLTNWERELEL *F FT WAPELYCVTYVGGKTARAVIRKHEISFEEVTTKTMRENQTQYKFNVMLTSYEFISVDAA *F FT FLGCIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKLLLTGTPLQNNLEELFHLLNFL *F FT SSGKFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRLKADVLKSMPPKSEFIVRVEL *F FT SSMQKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKCCNHPYLFPSAAEEATISPS *F FT GLYEMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKMLNILEHFLEGEGYQYDRI *F FT DGSIKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATADTVIIFDSDWNPHNDVQ *F FT AFSRAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLVVRPGMGGMTTNFSKDE *F FT LEDILRFGTEDLFKDGKSEAIHYDDKAVADLLDRTNRGIEEKESWANEYLSSFKVASYA *F FT TKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSLGKGKRVRKQVNYTDG *F FT GVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAERKAKRRLERRDDRP *F FT LPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLVRDLRGKSERNFKA *F FT YVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHINGYYS *F FT MPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSAAPSPAPASEKG *F FT EDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDTKPSDAEVKTE *F FT VAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMIVKEDGELEK *F FT PSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELHTLWLNEEK *F FT AAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVGKGNFLEI *F FT KNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAESHQHLS *F FT KESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSERSILSR *F FT LAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNSGV' *F XX *F SQ Sequence 4912 BP; 1322 A; 1234 C; 1340 G; 1016 T; 0 other; *F ctcaaatagc atgtcgtcta agagaggggc tgatccagac tggaaaacac ctggcaaggc 60 *F ttccaaggac aagcggccga aaaccaatgc caagaagcag aagtttcgcg acgaagaata 120 *F ctgcaaagtt tgtagtgatg gaggagatct gctgtgctgc gattcctgcc cttccgttta 180 *F ccacagaacc tgtctgagtc ctcccttgaa atcgattccc aaaggcgatt ggatctgccc 240 *F gcgctgcatt cccttgcccg gaaaagccga gaagatattg tcatggcgct gggccctcga 300 *F tcgaagcgtg gagcttcgta catctaaagg tgaaaagcgg cgcgagtact ttatcaagtg 360 *F gcatggcatg tcctactggc actgcgaatg gattcccgaa ggtcaaatgc tcttgcacca 420 *F tgcctcaatg gttgccagct tccagagaag gagcgacatg gaagagccaa gtctcgagga 480 *F gctcgatgat caggacggca atctgcacga gcgattctac agatacggca ttaagccgga 540 *F gtggctcctt gtccagcggg ttatcaatca tagtgaggag cccaatggag ggactatgta 600 *F cttggtgaaa tggcgtgagt tgtcttataa cgatagcagc tgggaaagag aaagcgacag 660 *F catcccgggt ctaaaccagg ctatcgctct ctacaagaaa ctacggtcca gtaataaggg 720 *F acgtcaaaga gataggcctg cacccacaat agacctaaat aagaagtacg aggatcagcc 780 *F agtattcttg aaggaggctg gccttaaatt gcatcccttt cagatagagg gcgtcagttg 840 *F gcttcgctat agttggggcc aaggcatccc caccatactg gccgacgaga tgggtctggg 900 *F caagaccatt cagaccgtgg tttttcttta ctcactattt aaggagggac attgccgcgg 960 *F acccttcctc atatccgtgc cgctttccac actgaccaat tgggagcgag agctggagct 1020 *F ctgggctcca gaactatact gtgtcaccta tgtgggcggc aagactgcta gagcggttat 1080 *F ccggaaacat gagataagct tcgaggaggt aaccaccaaa accatgcgcg aaaaccaaac 1140 *F ccagtataag tttaacgtaa tgctgaccag ctacgagttt atctctgtgg acgcagcctt 1200 *F tctcgggtgc atcgattggg cggcgctggt cgtggacgag gcccatcggc taaggagcaa 1260 *F ccagagcaag ttttttagga tcctgagcaa gtaccgcatc ggctacaagc tgctgctgac 1320 *F cggcactccg cttcagaaca atctcgagga gctgttccat ctgctcaatt tcctgagctc 1380 *F tggaaagttt aacgacctgc agaccttcca ggccgagttc actgacgttt cgaaggaaga 1440 *F gcaagtgaag cgactgcacg agatcttgga accgcatatg ctccgtcgcc ttaaggcgga 1500 *F tgtattgaag agcatgcccc cgaagtcgga gttcattgtg cgcgtggagc tgtcgtctat 1560 *F gcaaaaaaaa ttctacaagc atattttgac caaaaacttc aaggccttaa atcagaaagg 1620 *F cggcggtcgc gtgtgttcgc tgctcaacat catgatggac ctgagaaagt gctgcaatca 1680 *F tccgtacctg ttcccctccg ccgccgagga ggccactatc tcgccaagtg gtctttacga 1740 *F gatgagttcg ctgaccaaag cttccggcaa gctagattta ctgtcaaaga tgctaaaaca 1800 *F gctaaaggcg gacaatcaca gggtcctttt attctcccag atgaccaaga tgctgaatat 1860 *F tctcgagcac ttcctcgaag gggagggata ccagtacgat cgcatcgacg ggtcgatcaa 1920 *F aggcgattta cgtcagaagg ccattgacag attcaatgat cccgtatcgg agcactttgt 1980 *F cttcctgctg agcactcgtg caggcggact gggtatcaat ttggcaacag ccgacaccgt 2040 *F cattattttc gactcggact ggaatccgca caacgacgtt caggcctttt cccgagccca 2100 *F tcgcatgggg cagaaaaaga aggtaatgat ctaccgattt gtgacccaca actcggtgga 2160 *F ggagcgcatt atgcaggttg ccaagcacaa gatgatgctc acccatcttg tggtgcgccc 2220 *F gggaatgggt ggcatgacaa caaatttctc aaaggacgag ctcgaagaca tccttcgctt 2280 *F tggcaccgag gatcttttta aggacggcaa gagtgaggct attcactacg acgacaaagc 2340 *F ggtggccgat ctgcttgacc gcaccaatcg tggcattgaa gagaaggagt cttgggccaa 2400 *F cgagtatctt tcgtcgttta aggtggcttc gtacgccact aaagaagagg aggaggagga 2460 *F agagacggag attattaaac aggatgccga gaactctgat ccggcgtact gggtgaagct 2520 *F gctgcggcat cactatgaac agcaccagga agatgtgggt cgcagcctgg gcaagggcaa 2580 *F gcgcgtccgc aaacaggtca actacacgga tggcggcgtt gtggcggctg ataccacgcg 2640 *F ggatgattcc aactggcagg acaacggcag cgagtataac tccgagtatt cagctggctc 2700 *F cgatgaggat ggcggtgacg atgacttcga tgaccagaat ggcgcagaga gaaaggccaa 2760 *F gcggcgattg gagcgtcgcg acgatcgtcc attgcctcca ctgttggccc gcgtgggtgg 2820 *F aaacatcgag gtgctcggtt ttaatgcccg tcaacgcaag agcttcctca atgccattat 2880 *F gcggtacgga atgccgccgc aagatgcctt caactcgcaa tggctagtcc gggatttgcg 2940 *F tggtaaatcg gagcgcaatt ttaaggcgta tgtttctctg tttatgcggc atctttgcga 3000 *F gccgggagcg gacaatgcgg aaacctttgc cgatggtgtg ccgcgcgaag gactatcccg 3060 *F ccagcatgtg ctcacccgga tcggagtgat gtcgctaatt cgaaagaagg tacaggaatt 3120 *F cgagcatatt aacggatact acagcatgcc ggagctcatt ctcaagccgt gcgagcccgt 3180 *F acgatcagcc ctgaaacagg atgtggcggc acttgaagct ccgcctaccg gtggcaatgt 3240 *F cgacaagagc gctactacta gcaatagcgt cactccagcc accagtgcgg ctcccagtcc 3300 *F agcgccagcc tctgaaaaag gtgaagacaa agacaaggac tccgaaaagg agaaggataa 3360 *F gacctcggcg gagaagagcg aggtgaagca agaacaagag gctgaagagg acaagaagcc 3420 *F tggggatgtc aagcaggaga accctgtgga agaagctgcc ggcgacacaa agcccagcga 3480 *F cgcggaggtc aaaaccgagg tggctaaaac agaacccaag gaagagacca aggacccgga 3540 *F ggtaaaggag gagcccaaga ccgaagagaa ggaaaaagaa aaggtggatg acaagaaacc 3600 *F aataccaccg acgacagtaa ttgacgacga cgatgacgat gtgatgattg tcaaggagga 3660 *F cggtgagcta gaaaagccca gtgccagttc acccaaggat caaaaggcag tcgccgctgc 3720 *F tacgtctgct gcaactggag ccactggcaa gggagcggag gacagcttgg aggtgctaaa 3780 *F gcgcaaattt atgttcaaca tcgccgacgg cgggttcacc gaactgcaca ctttgtggct 3840 *F caacgaggag aaggctgccg ttcctgggcg agaatacgag atctggcacc gtcgccacga 3900 *F ctactggctg ttggccggaa ttgtgaccca tggctatggc cgttggcagg atatacaaaa 3960 *F cgatatccgt ttcgcgatca taaacgaacc tttcaagatg gacgttggta agggcaactt 4020 *F tctggaaatc aaaaacaagt tcctggcccg gcgttttaag ttgctggagc aggcgctggt 4080 *F catcgaggag cagctgcggc gagcggccta ccttaacctc gcccaggacc ccagccaccc 4140 *F ggcaatgtcg ctaaacgccc gtttcgcaga ggtcgaatgc ctggctgaat cccatcagca 4200 *F tctgagcaag gaatcgctgg ccggtaacaa gcccgcaaac gctgttctgc acaaggtgct 4260 *F caaccagctc gaagagctgc tctcggacat gaagagcgat gtgtcccggc tgccagctac 4320 *F actggctcgc atcccacctg ttgcgcaacg gcttcagatg tccgagagat cgattctctc 4380 *F ccgactggcg gccaccgccg gaaatgcctc caatgcagct caattgatgg cacaattccc 4440 *F ggctggattc cagggcacaa cattgccagc attcacgagc ggaccggctg gcaactttgc 4500 *F caactttcgg ccacagttct cggtgcccgg ccagctatcg aataattccg gcgtctaggc 4560 *F atctcctcaa tatctatgtt aagagcattc gtattccatg caaatatact ttactcaagc 4620 *F taagaattta aatttctaca cgatatcaag aaacaaaaaa tggacccacg tcgcgttcac 4680 *F acaaataatt aatgcaaaac gtttgacact tggcgaatgg cgagttcggt gtttccaggc 4740 *F ttcggtttct gctcctgggg cggatcggat ctcagcagag cacgactcgc ggtcagttca 4800 *F catacgacaa gtacaatagt tcttattaac ttctctacta tgtgattaca taatctagat 4860 *F acaaatataa atgtatttaa ttccattgca aaaaaaaaaa aaaaaaaaaa aa 4912 *F // *F \**B *F AF007780 ------------------------------------------------------------ *F AF119716 GATATTTGTTGAAGCCGCTTACAATTGACTATTTTTTTCTCGTCGTGTCTGTGTGTGCTT 60 *F *F *F AF007780 ------------------------------------CTCAAATAGCAT------------ 12 *F AF119716 AATTATTTGTTGTGGAAATTAAGACAATTTCGTGTGCTCAAACAGTGTAAAAGTTGCATT 120 *F \****** \** \* *F *F AF007780 ------------------------------------------------------------ *F AF119716 CGAGTGCTACAATGGCATCGGAGGAAGAGAATGACGATAATTTCCAGGAAGAAGAAGAGG 180 *F *F *F AF007780 ----------------------------GTCGTCTAA----------------------- 21 *F AF119716 CCCAGGAGGACAACGCACCTGCGGCGGAGCTGTCCAACGATTCCGACGCCCCTCTGAAGC 240 *F \* \*** \** *F *F AF007780 ------------------------------------------------------------ *F AF119716 CGAATAACGACGAAGACGACGACTACGATCCGGAGGACAGTCGCAGGAAGAAGAAGGGCA 300 *F *F *F AF007780 --------------------------GAGAGGGGCTG----------------------- 32 *F AF119716 AGAAGCGCAAGACCAGGAAGGGCGAAGAGAAGGGTCGCAAGAAGAAGAAGCGCAAGAAGA 360 *F \**** \*** \* *F *F AF007780 ------------------------------------------------------------ *F AF119716 ACGAGAGCGAAGAGGACAGCGACTTTGTCCAGCACGATGAGGAGGTGGAGTACCCCAGCA 420 *F *F *F AF007780 ------------------------------------------------------------ *F AF119716 CCTCCAAGCGCGCTCGCAAGCGCAAGGAGGAGAAACAGGCCGCCAAGGAGAAGGAGTCGG 480 *F *F *F AF007780 ----ATCCAGACTG---------GAAAACACCTG-------------------------- 53 *F AF119716 CATCATCCGGAATGCCATCTGTGGAAGACGTGTGCTCAGCCTTTAGCGTCTGCAATGTGG 540 *F \**** \** \** \*** \** \** *F *F AF007780 ------------------------------------------GCAAGGCTTCCA------ 65 *F AF119716 AGATCGAGTACAGTGAGGAGGAGCTGCAGAGCCTAACGACCTACAAGGCTTTCATGCATC 600 *F \******** \** *F *F AF007780 ---------------------AGGA----------------------------------- 69 *F AF119716 ATGTTCGTCCGATTCTGCAGAAGGAAAACCCCAAGATTGCGGCACCAAAGCTAGTGATGC 660 *F \**** *F *F AF007780 ----------CAAGCGGC----------CGAAAAC------------------------- 84 *F AF119716 TGGTGGCAGCCAAGTGGCGTGAGTTCTGCGAGAGCAATCCGCACATCCAGCAGGAGGGTG 720 *F \**** \*** \*** \* \* *F *F AF007780 -------------------------------------CAATGC----------------- 90 *F AF119716 GTGCCGCCGGCTCCGGTGGATCCGCCGGACAGGCACGCAGTGTGACAGGCGATGAGCCGG 780 *F \** \** *F *F AF007780 ------------------------------------------------------------ *F AF119716 AGGAACCTCGATCATCGCGTTCCTCGCGCAACGAGAAACCGGATGACATTTACGAGGAGG 840 *F *F *F AF007780 ------------------------------------------------------------ *F AF119716 CCGTCGAGGAGGAGGAAGAAGAGGAGGAGGAGGAGAAGAAACCTCGTCGTAAGCGCAGTG 900 *F *F *F AF007780 -------CAAGAAG---------------------------------------------- 97 *F AF119716 GACGTGGCAAGAAGGGGCGTCGTCCCTCCGGCAAGGTGCCCACTCTTAAGATTAAGCTGC 960 *F \******* *F *F AF007780 ------------------------------------------------------------ *F AF119716 TCGGCAAGCGCAAACGCGATAGTTCGGATGAGGAGCAAGATGCTAGCGGTGCATCGGAAC 1020 *F *F *F AF007780 ------------------------------------------------------------ *F AF119716 GCGATTCGGATCTTGAGTTTGAACGGATGCTTCAGAAATCGGATGACAGTGCCGATGAAA 1080 *F *F *F AF007780 ------------------------------------------------------------ *F AF119716 AGGAGGCTCCTGTCTCCTCTAAGGCGGACAACTCAGCACCCGCTGCCCAGGACGATGGAT 1140 *F *F *F AF007780 CAGAAGTTTC------GCG----------------------------------------- 110 *F AF119716 CAGGAGCTCCAGTGGTGCGCAAGAAGGCCAAGACCAAGATCGGCAACAAGTTCAAGAAGA 1200 *F \*** \** \* \* \*** *F *F AF007780 -----------------ACGAAGAA----------------------------------- 118 *F AF119716 AGAATAAACTCAAGAAAACGAAGAACTTCCCGGAGGGCGAAGATGGCGAGCATGAGCACC 1260 *F \******** *F *F AF007780 -----TACTGCAAAGTTTGT---------------------------------------- 133 *F AF119716 AGGACTACTGCGAGGTGTGCCAGCAAGGAGGTGAGATCATCCTGTGCGACACCTGCCCTC 1320 *F \****** \* \** \** *F *F AF007780 ------------------------------------------------------AGTGAT 139 *F AF119716 GGGCATATCACTTGGTGTGCCTGGAGCCAGAACTGGATGAACCGCCAGAGGGCAAGTGGT 1380 *F \**** \* *F *F AF007780 -------------------------------------GGAGGAGATC------------- 149 *F AF119716 CGTGTCCGCACTGCGAGGCTGACGGAGGTGCTGCTGAGGAAGAGGACGATGATGAGCACC 1440 *F \*** \*** \* *F *F AF007780 ------------------------------------TGCTGTGCTGCGATTCCTGCCCTT 173 *F AF119716 AGGAATTCTGCCGCGTGTGCAAGGACGGTGGCGAGTTGCTGTGCTGCGACTCATGTCCCT 1500 *F \************* \** \** \** \* *F *F AF007780 CCGTTTACCACAGAACCTGTCTGAGTCCTCCCTTGAAATCGATTCCCAAAGGCGATTGGA 233 *F AF119716 CCGCCTATCACACATTCTGCTTGAATCCGCCTCTGGACACTATACCCGATGGCGATTGGC 1560 *F \*** \** \**** \* \*** \*** \*** \** \** \* \* \** \*** \* \********* *F *F AF007780 TCTGCCCGCGCTGCA--TTCCCTT-GCCC---GGAAAAGCCGAGAAGAT-ATTGTCATGG 286 *F AF119716 GCTGTCCTCGTTGCAGCTGCCCTCCGCTCACTGGCAAGGCTGAGAAGATCATCACC-TGG 1619 *F \*** \** \** \**** \* \**** \** \* \** \** \** \******** \** \* \*** *F *F AF007780 CGCTGGGCCCTCGATCGAAGCGTGGA-GCTTCGTACATCTAAAGGTGA--AAAG------ 337 *F AF119716 CGATGGGCACAGCGTAGCAATGACGACGGTCCCTCCACCTCGAAGGGATCAAAGAACAGT 1679 *F \** \***** \* \* \* \* \* \** \* \* \* \* \** \** \* \* \** \**** *F *F AF007780 ------CGG---CGCGAGTACTTTATCAAGTGGCATGGCATGTCCTACTGGCACTGCGAA 388 *F AF119716 AACTCCCGGGTGCGCGAGTACTTTATCAAGTGGCACAACATGTCCTACTGGCACTGCGAA 1739 *F \*** \*********************** \********************** *F *F AF007780 TGGATTCCCGAAGGTCAAATGCTCTTGCACCATGCCTCAATGGTT-GCCAGCTTCCAGAG 447 *F AF119716 TGGGTGCCCGAAGTGCAACTGGACGTCCATCATCCGCTCATGATTCGTTCG-TTCCAGCG 1798 *F \*** \* \******* \*** \** \* \* \** \*** \* \*** \** \* \* \****** \* *F *F AF007780 AAGGAGCGACATGGAAGAGCC----AAGTCTCGAGGAG---CTCGATGA----------- 489 *F AF119716 CAAGTACGACATGGAAGAACCGCCCAAGT-TCGAGGAGTCGCTGGATGAGGCGGACACTC 1857 *F \* \* \************ \** \**** \******** \** \***** *F *F AF007780 -------------TCAG-----------------------GACGGCAATC---------- 503 *F AF119716 GTTACAAGCGTATTCAGCGACACAAGGACAAGGTTGGCATGAAGGCAAACGATGATGCCG 1917 *F \**** \** \***** \* *F *F AF007780 ------TGCACGAGCGATTCTACAGATACGGCATTAAGCCGGAGTGGCTCCTTGTCCAGC 557 *F AF119716 AAGTGCTGGAGGAGCGGTTCTACAAGAATGGCGTCAAGCCAGAGTGGCTCATTGTTCAGC 1977 *F \** \* \***** \******* \* \*** \* \***** \********* \**** \**** *F *F AF007780 GGGTTATCAATCATAGTGAGGAGCCCAATGGAGGGACTATGTACTTGGTGAAATGGCGTG 617 *F AF119716 GCGTAATCAACCATCGTACGGCAAGAGATGGCAGCACCATGTATCTGGTGAAGTGGCGCG 2037 *F \* \** \***** \*** \** \** \**** \* \** \***** \******* \***** \* *F *F AF007780 AGTTGTCTTATAACGATAGCAGCTGGGAAAGAGAAAGCGACAGCATCCCGGGTCTAAACC 677 *F AF119716 AGTTGCCCTATGACAAGTCCACCTGGGAAGAAGAGGGTGATGACATACAGGGCCTGCGGC 2097 *F \***** \* \*** \** \* \** \******* \*** \* \** \*** \* \*** \** \* *F *F AF007780 AGGCTATCGCTCTCTACAAGAAACTACG---------------------------GTC-- 708 *F AF119716 AAGCCATCGATTACTATCAGGATCTGCGTGCGGTTTGCACCTCGGAGACAACTCAGTCGC 2157 *F \* \** \**** \* \*** \** \* \** \** \*** *F *F AF007780 -CAGTAATAAG-----------------------------------GGACGTCAAAGA-- 730 *F AF119716 GCAGCAAAAAGAGCAAGAAGGGTCGCAAGTCCAAGCTCAAGGTCGAGGACGACGAGGATC 2217 *F \*** \** \*** \***** \* \* \** *F *F AF007780 -----GATAGGC-CTGCACCC-----------------ACAATAGACCTAAATAAGAAGT 767 *F AF119716 GCCCGGTCAAGCACTACACACCGCCGCCGGAAAAGCCAACTACTGATCTGAAGAAGAAGT 2277 *F \* \* \** \** \*** \* \** \* \** \** \** \******* *F *F AF007780 ACGAGGATCAGCCAGTATTCTTGAAGGAGGCTGGCCTTAAATTGCATCCCTTTCAGATAG 827 *F AF119716 ATGAGGATCAGCCGGCTTTCTTGGAGGGCACTGGCATGCAATTGCATCCCTACCAGATCG 2337 *F \* \*********** \* \****** \*** \***** \* \************ \***** \* *F *F AF007780 AGGGCGTCAGTTGGCTTCGCTATAGTTGGGGCCAAGGCATCCCCACCATACTGGCCGACG 887 *F AF119716 AGGGCATTAACTGGTTGCGCTACAGCTGGGGCCAGGGCATCGACACCATCCTGGCCGACG 2397 *F \***** \* \* \*** \* \***** \** \******** \****** \****** \********** *F *F AF007780 AGATGGGTCTGGGCAAGACCATTCAGACCGTGGTTTTTCTTTACTCACTATTTAAGGAGG 947 *F AF119716 AGATGGGTCTGGGTAAGACCATTCAGACGGTCACCTTCCTGTACTCTTTGTACAAGGAGG 2457 *F \************* \************** \** \** \** \***** \* \* \******* *F *F AF007780 GACATTGCCGCGGACCCTTCCTCATATCCGTGCCGCTTTCCACACTGACCAATTGGGAGC 1007 *F AF119716 GCCATTGCCGCGGACCCTTCCTCGTGGCCGTGCCACTCTCGACGCTAGTGAACTGGGAGC 2517 *F \* \********************* \* \******* \** \** \** \** \** \******* *F *F AF007780 GAGAGCTGGAGCTCTGGGCTCCAGAACTATACTGTGTCACCTATGTGGGCGGCAAGACTG 1067 *F AF119716 GTGAGTTTGAGCTGTGGGCTCCGGACTTTTACTGCATCACGTATATCGGTGACAAGGACT 2577 *F \* \*** \* \***** \******** \** \* \***** \**** \*** \* \** \* \**** *F *F AF007780 CTAGAGCGGTTATCCGGAAACATGAGATAAGCTTCGAGGAGGTAACCAC---------CA 1118 *F AF119716 CGAGAGCGGTTATACGGGAGAACGAGCTGAGCTTCGAGGAGGGCGCCATTCGTGGTAGCA 2637 *F \* \*********** \*** \* \* \*** \* \************* \*** \** *F *F AF007780 AAACCATGCGC--GAAAACCAAACCCAGTATAAGTTTAACGTAATGCTGACCAGCTACGA 1176 *F AF119716 AGGTTTCGCGTTTGCGGACC--ACCCAGTATAAATTCAACGTACTGCTGACTAGCTACGA 2695 *F \* \*** \* \*** \*********** \** \****** \******* \******** *F *F AF007780 GTTTATCTCTGTGGACGCAGCCTTTCTCGGGTGCATCGATTGGGCGGCGCTGGTCGTGGA 1236 *F AF119716 GCTGATCTCCATGGACGCTGCTTGCCTTGGCAGCATTGACTGGGCCGTGCTCGTGGTGGA 2755 *F \* \* \***** \******* \** \* \** \** \**** \** \***** \* \*** \** \***** *F *F AF007780 CGAGGCCCATCGGCTAAGGAGCAACCAGAGCAAGTTTTTTAGGATCCTGAGCAAGTACCG 1296 *F AF119716 CGAGGCCCATCGCTTGAAGAGCAACCAGAGCAAGTTCTTCCGTATCCTGAACAGCTACAC 2815 *F \************ \* \* \****************** \** \* \******* \** \*** *F *F AF007780 CATCGGCTACAAGCTGCTGCTGACCGGCACTCCGCTTCAGAACAATCTCGAGGAGCTGTT 1356 *F AF119716 TATTGCCTACAAGCTTCTGCTCACCGGCACACCGCTGCAGAACAATCTCGAGGAGCTGTT 2875 *F \** \* \********* \***** \******** \***** \*********************** *F *F AF007780 CCATCTGCTCAATTTCCTGAGCTCTGGAAAGTTTAACGACCTGCAGACCTTCCAGGCCGA 1416 *F AF119716 CCATCTGCTCAACTTCTTGAGCCGCGACAAGTTCAATGATCTGCAGGCCTTCCAGGGCGA 2935 *F \************ \*** \***** \* \***** \** \** \****** \********* \*** *F *F AF007780 GTTCACTGACGTTTCGAAGGAAGAGCAAGTGAAGCGACTGCACGAGATCTTGGAACCGCA 1476 *F AF119716 ATTCGCTGACGTTTCGAAGGAGGAGCAGGTTAAGCGTCTGCACGAGATGCTTGGACCGCA 2995 *F \*** \**************** \***** \** \***** \*********** \* \* \****** *F *F AF007780 TATGCTCCGTCGCCTTAAGGCGGATGTATTGAAGAGCATGCCCCCGAAGTCGGAGTTCAT 1536 *F AF119716 CATGCTGCGTCGTTTGAAGACGGATGTGCTGAAGAACATGCCGTCCAAGTCTGAGTTTAT 3055 *F \***** \***** \* \*** \******* \****** \****** \* \***** \***** \** *F *F AF007780 TGTGCGCGTGGAGCTGTCGTCTATGCAAAAAAAATTCTACAAGCATATTTTGACCAAAAA 1596 *F AF119716 CGTGCGCGTCGAGCTGTCGGCCATGCAGAAAAAGTTCTACAAGTTCATCTTGACCAAGAA 3115 *F \******** \********* \* \***** \***** \********* \** \******** \** *F *F AF007780 CTTCAAGGCCTTAAATCAGAAAGGCGGCGGTCGCGTGTGTTCGCTGCTCAACATCATGAT 1656 *F AF119716 CTACGAGGCTTTGAATTCGAAGAGCGGTGGTGGCTCTTGCTCACTGATCAACATTATGAT 3175 *F \** \* \**** \** \*** \*** \**** \*** \** \** \** \*** \******* \***** *F *F AF007780 GGACCTGAGAAAGTGCTGCAATCATCCGTACCTGTTCCCCTCCGCCGCCGAGGAGGCCAC 1716 *F AF119716 GGACCTAAAGAAGTGCTGCAACCATCCGTATCTCTTCCCCTCGGCCGCCGAGGAGGCAAC 3235 *F \****** \* \*********** \******** \** \******** \************** \** *F *F AF007780 TATCTCGCCAAGTGGTCTTTACGAGATGAGTTCGCTGACCAAAGCTTCCGGCAAGCTAGA 1776 *F AF119716 CACCGCTGCCGGTGGTCTCTACGAGATTAACTCGCTGACCAAGGCTGCTGGCAAGCTGGT 3295 *F \* \* \* \* \******* \******** \* \*********** \*** \* \******** \* *F *F AF007780 TTTACTGTCAAAGATGCTAAAACAGCTAAAGGCGGACAATCACAGGGTCCTTTTATTCTC 1836 *F AF119716 CCTGCTATCCAAGATGCTGAAGCAGCTGAAGGCACAAAACCATCGTGTCTTGATTTTCTC 3355 *F \* \** \** \******** \** \***** \***** \* \** \** \* \*** \* \* \***** *F *F AF007780 CCAGATGACCAAGATGCTGAATATTCTCGAGCACTTCCTCGAAGGGGAGGGATACCAGTA 1896 *F AF119716 CCAGATGACCAAGATGTTGGACATTCTCGAGGACTTCCTCGAGGGCGAGCAGTACAAGTA 3415 *F \**************** \** \* \********* \********** \** \*** \*** \**** *F *F AF007780 CGATCGCATCGACGGGTCGATCAAAGGCGATTTACGTCAGAAGGCCATTGACAGATTCAA 1956 *F AF119716 CGAACGAATTGACGGTGGAATCACAGGAACATTGCGTCAGGAAGCCATTGATAGGTTCAA 3475 *F \*** \** \** \***** \**** \*** \** \****** \* \******** \** \***** *F *F AF007780 TGATCCCGTATCGGAGCACTTTGTCTTCCTGCTGAGCACTCGTGCAGGCGGACTGGGTAT 2016 *F AF119716 TGCACCCGGAGCGCAGCAGTTTGTCTTCTTGCTGAGTACTCGAGCCGGAGGATTGGGTAT 3535 *F \** \**** \* \** \**** \********* \******* \***** \** \** \*** \******* *F *F AF007780 CAATTTGGCAACAGCCGACACCGTCATTATTTTCGACTCGGACTGGAATCCGCACAACGA 2076 *F AF119716 TAATTTGGCCACGGCCGACACTGTCATTATTTATGACTCCGATTGGAATCCCCACAACGA 3595 *F \******** \** \******** \********** \***** \** \******** \******** *F *F AF007780 CGTTCAGGCCTTTTCCCGAGCCCATCGCATGGGGCAGAAAAAGAAGGTAATGATCTACCG 2136 *F AF119716 TATTCAGGCCTTCTCCCGAGCCCATCGTATTGGCCAGGCTAACAAGGTGATGATCTATCG 3655 *F \********** \************** \** \** \*** \** \***** \******** \** *F *F AF007780 ATTTGTGACCCACAACTCGGTGGAGGAGCGCATTATGCAGGTTGCCAAGCACAAGATGAT 2196 *F AF119716 ATTTGTTACTCGAAATTCTGTGGAGGAGCGCGTTACCCAGGTGGCCAAGCGTAAGATGAT 3715 *F \****** \** \* \** \** \************ \*** \***** \******* \******** *F *F AF007780 GCTCACCCATCTTGTGGTGCGCCCGGGAATGGGTGGCATGACAACAAATTTCTCAAAGGA 2256 *F AF119716 GTTGACTCATCTTGTGGTCCGTCCGGGAATGGGCGGTAAAGGCGCTAACTTTACAAAGCA 3775 *F \* \* \** \*********** \** \*********** \** \* \* \** \** \***** \* *F *F AF007780 CGAGCTCGAAGACATCCTTCGCTTTGGCACCGAGGATCTTTTTAAGGA---CGGCAAGAG 2313 *F AF119716 AGAACTGGACGATATCCTTCGTTTCGGTACCGAGGATCTGTTCAAGGAGGATGACAAGGA 3835 *F \** \** \** \** \******** \** \** \*********** \** \***** \* \**** *F *F AF007780 TGAGGCTATTCACTACGACGACAAAGCGGTGGCCGATCTGCTTGACCGCACCAATCGTGG 2373 *F AF119716 GGAAGCTATCCACTATGACGACAAGGCGGTGGCCGAGCTGCTCGACAGAACCAACCGCGG 3895 *F \** \***** \***** \******** \*********** \***** \*** \* \***** \** \** *F *F AF007780 CATTGAAGAGAAGGAGTCTTGGGCCAACGAGTATCTTTCGTCGTTTAAGGTGGCTTCGTA 2433 *F AF119716 TATCGAGGAGAAAGAGTCCTGGGCCAACGAGTATCTGTCCTCGTTTAAGGTGGCTTCGTA 3955 *F \** \** \***** \***** \***************** \** \******************** *F *F AF007780 CGCCACTAAAGAAGAGGAGGAGGAGGAAGAGACGGAGATTATTAAACAGGATGCCGAGAA 2493 *F AF119716 CGCCACTAAAGAAGAGGAGGAGGAGGAAGAGACGGAGATTATTAAACAGGATGCCGAGAA 4015 *F \************************************************************ *F *F AF007780 CTCTGATCCGGCGTACTGGGTGAAGCTGCTGCGGCATCACTATGAACAGCACCAGGAAGA 2553 *F AF119716 CTCTGATCCGGCGTACTGGGTGAAGCTGCTGCGGCATCACTATGAACAGCACCAGGAAGA 4075 *F \************************************************************ *F *F AF007780 TGTGGGTCGCAGCCTGGGCAAGGGCAAGCGCGTCCGCAAACAGGTCAACTACACGGATGG 2613 *F AF119716 TGTGGGTCGCAGCCTGGGCAAGGGCAAGCGCGTCCGCAAACAGGTCAACTACACGGATGG 4135 *F \************************************************************ *F *F AF007780 CGGCGTTGTGGCGGCTGATACCACGCGGGATGATTCCAACTGGCAGGACAACGGCAGCGA 2673 *F AF119716 CGGCGTTGTGGCGGCTGATACCACGCGGGATGATTCCAACTGGCAGGACAACGGCAGCGA 4195 *F \************************************************************ *F *F AF007780 GTATAACTCCGAGTATTCAGCTGGCTCCGATGAGGATGGCGGTGACGATGACTTCGATGA 2733 *F AF119716 GTATAACTCCGAGTATTCAGCTGGCTCCGATGAGGATGGCGGTGACGATGACTTCGATGA 4255 *F \************************************************************ *F *F AF007780 CCAGAATGGCGCAGAGAGAAAGGCCAAGCGGCGATTGGAGCGTCGCGACGATCGTCCATT 2793 *F AF119716 CCAGAATGGCGCAGAGAGAAAGGCCAAGCGGCGATTGGAGCGTCGCGACGATCGTCCATT 4315 *F \************************************************************ *F *F AF007780 GCCTCCACTGTTGGCCCGCGTGGGTGGAAACATCGAGGTGCTCGGTTTTAATGCCCGTCA 2853 *F AF119716 GCCTCCACTGTTGGCCCGCGTGGGTGGAAACATCGAGGTGCTCGGTTTTAATGCCCGTCA 4375 *F \************************************************************ *F *F AF007780 ACGCAAGAGCTTCCTCAATGCCATTATGCGGTACGGAATGCCGCCGCAAGATGCCTTCAA 2913 *F AF119716 ACGCAAGAGCTTCCTCAATGCCATTATGCGGTACGGAATGCCGCCGCAAGATGCCTTCAA 4435 *F \************************************************************ *F *F AF007780 CTCGCAATGGCTAGTCCGGGATTTGCGTGGTAAATCGGAGCGCAATTTTAAGGCGTATGT 2973 *F AF119716 CTCGCAATGGCTAGTCCGGGATTTGCGTGGTAAATCGGAGCGCAATTTTAAGGCGTATGT 4495 *F \************************************************************ *F *F AF007780 TTCTCTGTTTATGCGGCATCTTTGCGAGCCGGGAGCGGACAATGCGGAAACCTTTGCCGA 3033 *F AF119716 TTCTCTGTTTATGCGGCATCTTTGCGAGCCGGGAGCGGACAATGCGGAAACCTTTGCCGA 4555 *F \************************************************************ *F *F AF007780 TGGTGTGCCGCGCGAAGGACTATCCCGCCAGCATGTGCTCACCCGGATCGGAGTGATGTC 3093 *F AF119716 TGGTGTGCCGCGCGAAGGACTATCCCGCCAGCATGTGCTCACCCGGATCGGAGTGATGTC 4615 *F \************************************************************ *F *F AF007780 GCTAATTCGAAAGAAGGTACAGGAATTCGAGCATATTAACGGATACTACAGCATGCCGGA 3153 *F AF119716 GCTAATTCGAAAGAAGGTACAGGAATTCGAGCATATTAACGGATACTACAGCATGCCGGA 4675 *F \************************************************************ *F *F AF007780 GCTCATTCTCAAGCCGTGCGAGCCCGTACGATCAGCCCTGAAACAGGATGTGGCGGCACT 3213 *F AF119716 GCTCATTCTCAAGCCGTGCGAGCCCGTACGATCAGCCCTGAAACAGGATGTGGCGGCACT 4735 *F \************************************************************ *F *F AF007780 TGAAGCTCCGCCTACCGGTGGCAATGTCGACAAGAGCGCTACTACTAGCAATAGCGTCAC 3273 *F AF119716 TGAAGCTCCGCCTACCGGTGGCAATGTCGACAAGAGCGCTACTACTAGCAATAGCGTCAC 4795 *F \************************************************************ *F *F AF007780 TCCAGCCACCAGTGCGGCTCCCAGTCCAGCGCCAGCCTCTGAAAAAGGTGAAGACAAAGA 3333 *F AF119716 TCCAGCCACCAGTGCGGCTCCCAGTCCAGCGCCAGCCTCTGAAAAAGGTGAAGACAAAGA 4855 *F \************************************************************ *F *F AF007780 CAAGGACTCCGAAAAGGAGAAGGATAAGACCTCGGCGGAGAAGAGCGAGGTGAAGCAAGA 3393 *F AF119716 CAAGGACTCCGAAAAGGAAAAGGATAAGACCTCGGCGGAGAAGAGCGAGGTGAAGCAAGA 4915 *F \****************** \***************************************** *F *F AF007780 ACAAGAGGCTGAAGAGGACAAGAAGCCTGGGGATGTCAAGCAGGAGAACCCTGTGGAAGA 3453 *F AF119716 ACAAGAGGCTGAAGAGGACAAGAAGCCTGGGGATGTCAAGCAGGAGAACCCTGTGGAAGA 4975 *F \************************************************************ *F *F AF007780 AGCTGCCGGCGACACAAAGCCCAGCGACGCGGAGGTCAAAACCGAGGTGGCTAAAACAGA 3513 *F AF119716 AGCTGCCGGCGACACAAAGCCCAGCGACGCGGAGGTCAAAACCGAGGTGGCTAAAACAGA 5035 *F \************************************************************ *F *F AF007780 ACCCAAGGAAGAGACCAAGGACCCGGAGGTAAAGGAGGAGCCCAAGACCGAAGAGAAGGA 3573 *F AF119716 ACCCAAGGAAGAGACCAAGGACCCGGAGGTAAAGGAGGAGCCCAAGACCGAAGAGAAGGA 5095 *F \************************************************************ *F *F AF007780 AAAAGAAAAGGTGGATGACAAGAAACCAATACCACCGACGACAGTAATTGACGACGACGA 3633 *F AF119716 AAAAGAAAAGGTGGATGACAAGAAACCAATACCACCGACGACAGTAATTGACGACGATGA 5155 *F \********************************************************* \** *F *F AF007780 TGACGATGTGATGATTGTCAAGGAGGACGGTGAGCTAGAAAAGCCCAGTGCCAGTTCACC 3693 *F AF119716 TGACGATGTGATGATTGTCAAGGAGGACGGTGAGCTAGAAAAGCCCAGTGCCAGTTCACC 5215 *F \************************************************************ *F *F AF007780 CAAGGATCAAAAGGCAGTCGCCGCTGCTACGTCTGCTGCAACTGGAGCCACTGGCAAGGG 3753 *F AF119716 CAAGGATCAAAAGGCAGTCGCCGCTGCTACGTCTGCTGCAACTGGAGCCACTGGCAAGGG 5275 *F \************************************************************ *F *F AF007780 AGCGGAGGACAGCTTGGAGGTGCTAAAGCGCAAATTTATGTTCAACATCGCCGACGGCGG 3813 *F AF119716 AGCGGAGGACAGCTTGGAGGTGCTAAAGCGCAAATTTATGTTCAACATCGCCGACGGCGG 5335 *F \************************************************************ *F *F AF007780 GTTCACCGAACTGCACACTTTGTGGCTCAACGAGGAGAAGGCTGCCGTTCCTGGGCGAGA 3873 *F AF119716 GTTCACCGAACTGCACACTTTGTGGCTCAACGAGGAGAAGGCTGCCGTTCCTGGGCGAGA 5395 *F \************************************************************ *F *F AF007780 ATACGAGATCTGGCACCGTCGCCACGACTACTGGCTGTTGGCCGGAATTGTGACCCATGG 3933 *F AF119716 ATACGAGATCTGGCACCGTCGCCACGACTACTGGCTGTTGGCCGGAATTGTGACCCATGG 5455 *F \************************************************************ *F *F AF007780 CTATGGCCGTTGGCAGGATATACAAAACGATATCCGTTTCGCGATCATAAACGAACCTTT 3993 *F AF119716 CTATGGCCGTTGGCAGGATATACAAAACGATATCCGTTTCGCGATCATAAACGAACCTTT 5515 *F \************************************************************ *F *F AF007780 CAAGATGGACGTTGGTAAGGGCAACTTTCTGGAAATCAAAAACAAGTTCCTGGCCCGGCG 4053 *F AF119716 CAAGATGGACGTTGGTAAGGGCAACTTTCTGGAAATCAAAAACAAGTTCCTGGCCCGGCG 5575 *F \************************************************************ *F *F AF007780 TTTTAAGTTGCTGGAGCAGGCGCTGGTCATCGAGGAGCAGCTGCGGCGAGCGGCCTACCT 4113 *F AF119716 TTTTAAGTTGCTGGAGCAGGCCCTGGTCATCGAGGAGCAGCTGCGGCGAGCCGCCTACCT 5635 *F \********************* \***************************** \******** *F *F AF007780 TAACCTCGCCCAGGACCCCAGCCACCCGGCAATGTCGCTAAACGCCCGTTTCGCAGAGGT 4173 *F AF119716 TAACCTCGCCCAAGACCCCAGCCACCCGGCAATGTCGCTAAACGCCCGTTTCGCAGAGGT 5695 *F \************ \*********************************************** *F *F AF007780 CGAATGCCTGGCTGAATCCCATCAGCATCTGAGCAAGGAATCGCTGGCCGGTAACAAGCC 4233 *F AF119716 CGAATGCCTGGCTGAATCCCATCAGCATCTGAGCAAGGAATCGCTGGCCGGTAACAAGCC 5755 *F \************************************************************ *F *F AF007780 CGCAAACGCTGTTCTGCACAAGGTGCTCAACCAGCTCGAAGAGCTGCTCTCGGACATGAA 4293 *F AF119716 CGCAAACGCTGTTTTGCACAAGGTGCTCAACCAGCTCGAAGAGCTGCTCTCGGACATGAA 5815 *F \************* \********************************************** *F *F AF007780 GAGCGATGTGTCCCGGCTGCCAGCTACACTGGCTCGCATCCCACCTGTTGCGCAACGGCT 4353 *F AF119716 GAGCGATGTGTCCCGGCTGCCAGCTACACTGGCTCGCATCCCACCTGTGGCGCAACGGCT 5875 *F \************************************************ \*********** *F *F AF007780 TCAGATGTCCGAGAGATCGATTCTCTCCCGACTGGCGGCCACCGCCGGAAATGCCTCCAA 4413 *F AF119716 TCAGATGTCCGAGAGATCGATTCTCTCCCGACTGGCGGCCACCGCCGGAAATGCCTCCAA 5935 *F \************************************************************ *F *F AF007780 TGCAGCTCAATTGATGGCACAATTCCCGGCTGGATTCCAGGGCACAACATTGCCAGCATT 4473 *F AF119716 TGCAGCTCAATTGATGGCACAATTCCCGGCTGGATTCCAGGGCACAACATTGCCAGCATT 5995 *F \************************************************************ *F *F AF007780 CACGAGCGGACCGGCTGGCAACTTTGCCAACTTTCGGCCACAGTTCTCGGTGCCCGGCCA 4533 *F AF119716 CACGAGCGGACCGGCTGGCAACTTTGCCAACTTTCGGCCACAGTTCTCGGTGCCCGGCCA 6055 *F \************************************************************ *F *F AF007780 GCTATCGAATAATTCCGGCGTCTAGGCATCTCCTCAATATCTATGTTAAGAGCATTCGTA 4593 *F AF119716 GCTATCGAATAATTCCGGCGTCTAGGCATCTCCTCAATATCTATGTTAAGAGCATTCGTA 6115 *F \************************************************************ *F *F AF007780 TTCCATGCAAATATACTTTACTCAAGCTAAGAATTTAAATTTCTACACG----------- 4642 *F AF119716 TTCCATGCAAATATACTTTACTCAAGCTAAGAATTTAAATTTCTACACGGTAACACACCC 6175 *F \************************************************* *F *F AF007780 --------------------------------------------------------ATAT 4646 *F AF119716 GCACACCCACACACCCACAACATATATTTATCATAATCATTTGCAATGAAATCCAGATAT 6235 *F \**** *F *F AF007780 CAAGAAACAAAAAATGGACCCACGTCGCGTTCACACAAATAATTAATGCAAAACGTTTGA 4706 *F AF119716 CAAGAAACAAAAAATGGACCCACGTCGCGTTCACACAAATAATTAATGCAAAACGTTTGA 6295 *F \************************************************************ *F *F AF007780 CACTTGGCGAATGGCGAGTTCGGTGTTTCCAGGCTTCGGTTTCTGCTCCTGGGGCGGATC 4766 *F AF119716 CACTTGGCGAATGGCGAGTTCGGTGTTTCCAGGCTTCGGTTTCTGCTCCTGGGGCGGATC 6355 *F \************************************************************ *F *F AF007780 GGATCTCAGCAGAGCACGACTCGCGGTCAGTTCACATACGACAAGTACAATAGTTCTTAT 4826 *F AF119716 GGATCTCAGCAGAGCACGACTCGCGGTCAGTTCACATACGACAAGTACAATAGTTCTTAT 6415 *F \************************************************************ *F *F AF007780 TAACTTCTCTACTATGTGATTACATAATCTAGATACAAATATAAATGTATTTAATTCCAT 4886 *F AF119716 TAACTTCTCTACTATGTGATTACATAATCTAGATACAAATATAAATGTATTTAATTCCAT 6475 *F \************************************************************ *F *F AF007780 TGCAAAAAAAAAAAAAAAAAAAAAAA 4912 *F AF119716 -------------------------- *F *F \**C *F >CG8103 |FBan0008103|CT24204|FBan0008103 last_updated:000321 *F AC AE003515; AE002602; *F XX *F SV AE003515.1 *F FT mRNA join(<162750..162759,162846..162922,162985..163228, *F FT 163356..165654,165950..168806,168872..169409) *F FT /note='Nucleotide sequence of the Celera sequence differs *F FT from the published sequence for this transcript.' *F FT /gene='Mi-2' *F FT /product='CT24204' *F FT CDS join(162750..162759,162846..162922,162985..163228, *F FT 163356..165654,165950..165974) *F FT /codon_start=1 *F FT /db_xref='FLYBASE:FBan0008103' *F FT /db_xref='FLYBASE:FBgn0013591' *F FT /note='Mi-2 gene product; Nucleotide sequence of the Celera *F FT sequence differs from the published sequence for this *F FT transcript' *F FT /gene='Mi-2' *F FT /protein_id='AAF49099.1' *F FT /translation='MAHEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKK *F FT KGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKE *F FT KESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAP *F FT KLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPD *F FT DIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQD *F FT ASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTK *F FT IGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPEL *F FT DEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPP *F FT LDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIK *F FT WHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKD *F FT KVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTW *F FT EEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTP *F FT PPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKT *F FT IQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIR *F FT ENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRL *F FT KSNQSKKSSTSSS' *F // *F >CG9594|FBan0009594|CT27128|FBan0009594 last_updated:000321 *F AC AE003517; AE002602; *F XX *F SV AE003517.1 *F FT CDS join(complement(7300..9814),complement(7131..7267)) *F FT /codon_start=1 *F FT /db_xref='FLYBASE:FBan0009594' *F FT /db_xref='FLYBASE:FBgn0023395' *F FT /note='Chd3 gene product; Nucleotide sequence of the Celera *F FT sequence differs from the published sequence for this *F FT transcript' *F FT /gene='Chd3' *F FT /protein_id='AAF49162.1' *F FT /translation='MSSKRGADPDWKTPGKASKDKRPKTNAKKQKFRDEEYCKVCSDGG *F FT DLLCCDSCPSVYHRTCLSPPLKSIPKGDWICPRCIPLPGKAEKILSWRWALDRSVELRT *F FT SKGEKRREYFIKWHGMSYWHCEWIPEGQMLLHHASMVASFQRRSDMEEPSLEELDDQDG *F FT NLHERFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLN *F FT QAIALYKKLRSSNKGRQRDRPAPTIDLNKKYEDQPVFLKEAGLKLHPFQIEGVSWLRYS *F FT WGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRGPFLISVPLSTLTNWERELELWAP *F FT ELYCVTYVGGKTARAVIRKHEISFEEVTTKTMRENQTQYKFNVMLTSYEFISVDAAFLG *F FT CIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKLLLTGTPLQNNLEELFHLLNFLSSG *F FT KFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRLKADVLKSMPPKSEFIVRVELSSM *F FT QKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKCCNHPYLFPSAAEEATISPSGLY *F FT EMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKMLNVLEHFLEGEGYQYDRIDGS *F FT IKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATADTVIIFDSDWNPHNDVQAFS *F FT RAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLVVRPGMGGMTTNFSKDELED *F FT ILRFGTEDLFKDGKSEAIHYDDKAVADLLDRTNRGIEEKESWANEYLSSFKVASYATKE *F FT DHEEHDDYNNDAENTDPFYWENLMGKTVAAVPGGRREHHGKGQANPKGNRLLQSISLSK *F FT SSHPKQYSINVR' *F // *F ID O16102 PRELIMINARY; PRT; 1518 AA. *F AC O16102; *F DT 01-JAN-1998 (TrEMBLrel. 05, Created) *F DT 01-JAN-1998 (TrEMBLrel. 05, Last sequence update) *F DT 01-MAY-1999 (TrEMBLrel. 10, Last annotation update) *F DE CHD3 (FRAGMENT). *F GN CHD3. *F OS Drosophila melanogaster (Fruit fly). *F OC Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F OC Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; *F OC Ephydroidea; Drosophilidae; Drosophila. *F RN [1] *F RP SEQUENCE FROM N.A. *F RX MEDLINE; 97470991. *F RA Woodage T., Basrai M.A., Baxevanis A.D., Hieter P., Collins F.S.; *F RT 'Characterization of the CHD family of proteins.'; *F RL Proc. Natl. Acad. Sci. U.S.A. 94:11472-11477(1997). *F DR EMBL; AF007780; AAB87384.1; -. *F DR FLYBASE; FBgn0023395; Chd3. *F DR PFAM; PF00271; helicase_C; 1. *F DR PFAM; PF00628; PHD; 1. *F DR PFAM; PF00176; SNF2_N; 1. *F FT NON_TER 1 1 *F SQ SEQUENCE 1518 AA; 172028 MW; 3873D071395AFA55 CRC64; *F SNSMSSKRGA DPDWKTPGKA SKDKRPKTNA KKQKFRDEEY CKVCSDGGDL LCCDSCPSVY *F HRTCLSPPLK SIPKGDWICP RCIPLPGKAE KILSWRWALD RSVELRTSKG EKRREYFIKW *F HGMSYWHCEW IPEGQMLLHH ASMVASFQRR SDMEEPSLEE LDDQDGNLHE RFYRYGIKPE *F WLLVQRVINH SEEPNGGTMY LVKWRELSYN DSSWERESDS IPGLNQAIAL YKKLRSSNKG *F RQRDRPAPTI DLNKKYEDQP VFLKEAGLKL HPFQIEGVSW LRYSWGQGIP TILADEMGLG *F KTIQTVVFLY SLFKEGHCRG PFLISVPLST LTNWERELEL WAPELYCVTY VGGKTARAVI *F RKHEISFEEV TTKTMRENQT QYKFNVMLTS YEFISVDAAF LGCIDWAALV VDEAHRLRSN *F QSKFFRILSK YRIGYKLLLT GTPLQNNLEE LFHLLNFLSS GKFNDLQTFQ AEFTDVSKEE *F QVKRLHEILE PHMLRRLKAD VLKSMPPKSE FIVRVELSSM QKKFYKHILT KNFKALNQKG *F GGRVCSLLNI MMDLRKCCNH PYLFPSAAEE ATISPSGLYE MSSLTKASGK LDLLSKMLKQ *F LKADNHRVLL FSQMTKMLNI LEHFLEGEGY QYDRIDGSIK GDLRQKAIDR FNDPVSEHFV *F FLLSTRAGGL GINLATADTV IIFDSDWNPH NDVQAFSRAH RMGQKKKVMI YRFVTHNSVE *F ERIMQVAKHK MMLTHLVVRP GMGGMTTNFS KDELEDILRF GTEDLFKDGK SEAIHYDDKA *F VADLLDRTNR GIEEKESWAN EYLSSFKVAS YATKEEEEEE ETEIIKQDAE NSDPAYWVKL *F LRHHYEQHQE DVGRSLGKGK RVRKQVNYTD GGVVAADTTR DDSNWQDNGS EYNSEYSAGS *F DEDGGDDDFD DQNGAERKAK RRLERRDDRP LPPLLARVGG NIEVLGFNAR QRKSFLNAIM *F RYGMPPQDAF NSQWLVRDLR GKSERNFKAY VSLFMRHLCE PGADNAETFA DGVPREGLSR *F QHVLTRIGVM SLIRKKVQEF EHINGYYSMP ELILKPCEPV RSALKQDVAA LEAPPTGGNV *F DKSATTSNSV TPATSAAPSP APASEKGEDK DKDSEKEKDK TSAEKSEVKQ EQEAEEDKKP *F GDVKQENPVE EAAGDTKPSD AEVKTEVAKT EPKEETKDPE VKEEPKTEEK EKEKVDDKKP *F IPPTTVIDDD DDDVMIVKED GELEKPSASS PKDQKAVAAA TSAATGATGK GAEDSLEVLK *F RKFMFNIADG GFTELHTLWL NEEKAAVPGR EYEIWHRRHD YWLLAGIVTH GYGRWQDIQN *F DIRFAIINEP FKMDVGKGNF LEIKNKFLAR RFKLLEQALV IEEQLRRAAY LNLAQDPSHP *F AMSLNARFAE VECLAESHQH LSKESLAGNK PANAVLHKVL NQLEELLSDM KSDVSRLPAT *F LARIPPVAQR LQMSERSILS RLAATAGNAS NAAQLMAQFP AGFQGTTLPA FTSGPAGNFA *F NFRPQFSVPG QLSNNSGV *F // *F ID O97159 PRELIMINARY; PRT; 1982 AA. *F AC O97159; *F DT 01-MAY-1999 (TrEMBLrel. 10, Created) *F DT 01-MAY-1999 (TrEMBLrel. 10, Last sequence update) *F DT 01-MAY-1999 (TrEMBLrel. 10, Last annotation update) *F DE DMI-2 PROTEIN. *F GN DMI-2. *F OS Drosophila melanogaster (Fruit fly). *F OC Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F OC Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; *F OC Ephydroidea; Drosophilidae; Drosophila. *F RN [1] *F RP SEQUENCE FROM N.A. *F RX MEDLINE; 99055400. *F RA Kehle J., Beuchle D., Treuheit S., Christen B., Kennison J.A., *F RA Bienz M., Muller J.; *F RT 'dMi-2, a hunchback-interacting protein that functions in polycomb *F RT repression.'; *F RL Science 282:1897-1900(1998). *F RN [2] *F RP SEQUENCE FROM N.A. *F RA Mueller J.; *F RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases. *F DR EMBL; AF119716; AAD17276.1; -. *F SQ SEQUENCE 1982 AA; 224213 MW; 078A13FFA00A1813 CRC64; *F MASEEENDDN FQEEEEAQED NAPAAELSND SDAPLKPNND EDDDYDPEDS RRKKKGKKRK *F TRKGEEKGRK KKKRKKNESE EDSDFVQHDE EVEYPSTSKR ARKRKEEKQA AKEKESASSG *F MPSVEDVCSA FSVCNVEIEY SEEELQSLTT YKAFMHHVRP ILQKENPKIA APKLVMLVAA *F KWREFCESNP HIQQEGGAAG SGGSAGQARS VTGDEPEEPR SSRSSRNEKP DDIYEEAVEE *F EEEEEEEEKK PRRKRSGRGK KGRRPSGKVP TLKIKLLGKR KRDSSDEEQD ASGASERDSD *F LEFERMLQKS DDSADEKEAP VSSKADNSAP AAQDDGSGAP VVRKKAKTKI GNKFKKKNKL *F KKTKNFPEGE DGEHEHQDYC EVCQQGGEII LCDTCPRAYH LVCLEPELDE PPEGKWSCPH *F CEADGGAAEE EDDDEHQEFC RVCKDGGELL CCDSCPSAYH TFCLNPPLDT IPDGDWRCPR *F CSCPPLTGKA EKIITWRWAQ RSNDDGPSTS KGSKNSNSRV REYFIKWHNM SYWHCEWVPE *F VQLDVHHPLM IRSFQRKYDM EEPPKFEESL DEADTRYKRI QRHKDKVGMK ANDDAEVLEE *F RFYKNGVKPE WLIVQRVINH RTARDGSTMY LVKWRELPYD KSTWEEEGDD IQGLRQAIDY *F YQDLRAVCTS ETTQSRSKKS KKGRKSKLKV EDDEDRPVKH YTPPPEKPTT DLKKKYEDQP *F AFLEGTGMQL HPYQIEGINW LRYSWGQGID TILADEMGLG KTIQTVTFLY SLYKEGHCRG *F PFLVAVPLST LVNWEREFEL WAPDFYCITY IGDKDSRAVI RENELSFEEG AIRGSKVSRL *F RTTQYKFNVL LTSYELISMD AACLGSIDWA VLVVDEAHRL KSNQSKFFRI LNSYTIAYKL *F LLTGTPLQNN LEELFHLLNF LSRDKFNDLQ AFQGEFADVS KEEQVKRLHE MLGPHMLRRL *F KTDVLKNMPS KSEFIVRVEL SAMQKKFYKF ILTKNYEALN SKSGGGSCSL INIMMDLKKC *F CNHPYLFPSA AEEATTAAGG LYEINSLTKA AGKLVLLSKM LKQLKAQNHR VLIFSQMTKM *F LDILEDFLEG EQYKYERIDG GITGTLRQEA IDRFNAPGAQ QFVFLLSTRA GGLGINLATA *F DTVIIYDSDW NPHNDIQAFS RAHRIGQANK VMIYRFVTRN SVEERVTQVA KRKMMLTHLV *F VRPGMGGKGA NFTKQELDDI LRFGTEDLFK EDDKEEAIHY DDKAVAELLD RTNRGIEEKE *F SWANEYLSSF KVASYATKEE EEEEETEIIK QDAENSDPAY WVKLLRHHYE QHQEDVGRSL *F GKGKRVRKQV NYTDGGVVAA DTTRDDSNWQ DNGSEYNSEY SAGSDEDGGD DDFDDQNGAE *F RKAKRRLERR DDRPLPPLLA RVGGNIEVLG FNARQRKSFL NAIMRYGMPP QDAFNSQWLV *F RDLRGKSERN FKAYVSLFMR HLCEPGADNA ETFADGVPRE GLSRQHVLTR IGVMSLIRKK *F VQEFEHINGY YSMPELILKP CEPVRSALKQ DVAALEAPPT GGNVDKSATT SNSVTPATSA *F APSPAPASEK GEDKDKDSEK EKDKTSAEKS EVKQEQEAEE DKKPGDVKQE NPVEEAAGDT *F KPSDAEVKTE VAKTEPKEET KDPEVKEEPK TEEKEKEKVD DKKPIPPTTV IDDDDDDVMI *F VKEDGELEKP SASSPKDQKA VAAATSAATG ATGKGAEDSL EVLKRKFMFN IADGGFTELH *F TLWLNEEKAA VPGREYEIWH RRHDYWLLAG IVTHGYGRWQ DIQNDIRFAI INEPFKMDVG *F KGNFLEIKNK FLARRFKLLE QALVIEEQLR RAAYLNLAQD PSHPAMSLNA RFAEVECLAE *F SHQHLSKESL AGNKPANAVL HKVLNQLEEL LSDMKSDVSR LPATLARIPP VAQRLQMSER *F SILSRLAATA GNASNAAQLM AQFPAGFQGT TLPAFTSGPA GNFANFRPQF SVPGQLSNNS *F GV *F // *F \**D *F >O97159 *F MASEEENDDN FQEEEEAQED NAPAAELSND SDAPLKPNND EDDDYDPEDS RRKKKGKKRK *F TRKGEEKGRK KKKRKKNESE EDSDFVQHDE EVEYPSTSKR ARKRKEEKQA AKEKESASSG *F MPSVEDVCSA FSVCNVEIEY SEEELQSLTT YKAFMHHVRP ILQKENPKIA APKLVMLVAA *F KWREFCESNP HIQQEGGAAG SGGSAGQARS VTGDEPEEPR SSRSSRNEKP DDIYEEAVEE *F EEEEEEEEKK PRRKRSGRGK KGRRPSGKVP TLKIKLLGKR KRDSSDEEQD ASGASERDSD *F LEFERMLQKS DDSADEKEAP VSSKADNSAP AAQDDGSGAP VVRKKAKTKI GNKFKKKNKL *F KKTKNFPEGE DGEHEHQDYC EVCQQGGEII LCDTCPRAYH LVCLEPELDE PPEGKWSCPH *F CEADGGAAEE EDDDEHQEFC RVCKDGGELL CCDSCPSAYH TFCLNPPLDT IPDGDWRCPR *F CSCPPLTGKA EKIITWRWAQ RSNDDGPSTS KGSKNSNSRV REYFIKWHNM SYWHCEWVPE *F VQLDVHHPLM IRSFQRKYDM EEPPKFEESL DEADTRYKRI QRHKDKVGMK ANDDAEVLEE *F RFYKNGVKPE WLIVQRVINH RTARDGSTMY LVKWRELPYD KSTWEEEGDD IQGLRQAIDY *F YQDLRAVCTS ETTQSRSKKS KKGRKSKLKV EDDEDRPVKH YTPPPEKPTT DLKKKYEDQP *F AFLEGTGMQL HPYQIEGINW LRYSWGQGID TILADEMGLG KTIQTVTFLY SLYKEGHCRG *F PFLVAVPLST LVNWEREFEL WAPDFYCITY IGDKDSRAVI RENELSFEEG AIRGSKVSRL *F RTTQYKFNVL LTSYELISMD AACLGSIDWA VLVVDEAHRL KSNQSKFFRI LNSYTIAYKL *F LLTGTPLQNN LEELFHLLNF LSRDKFNDLQ AFQGEFADVS KEEQVKRLHE MLGPHMLRRL *F KTDVLKNMPS KSEFIVRVEL SAMQKKFYKF ILTKNYEALN SKSGGGSCSL INIMMDLKKC *F CNHPYLFPSA AEEATTAAGG LYEINSLTKA AGKLVLLSKM LKQLKAQNHR VLIFSQMTKM *F LDILEDFLEG EQYKYERIDG GITGTLRQEA IDRFNAPGAQ QFVFLLSTRA GGLGINLATA *F DTVIIYDSDW NPHNDIQAFS RAHRIGQANK VMIYRFVTRN SVEERVTQVA KRKMMLTHLV *F VRPGMGGKGA NFTKQELDDI LRFGTEDLFK EDDKEEAIHY DDKAVAELLD RTNRGIEEKE *F SWANEYLSSF KVASYATKEE EEEEETEIIK QDAENSDPAY WVKLLRHHYE QHQEDVGRSL *F GKGKRVRKQV NYTDGGVVAA DTTRDDSNWQ DNGSEYNSEY SAGSDEDGGD DDFDDQNGAE *F RKAKRRLERR DDRPLPPLLA RVGGNIEVLG FNARQRKSFL NAIMRYGMPP QDAFNSQWLV *F RDLRGKSERN FKAYVSLFMR HLCEPGADNA ETFADGVPRE GLSRQHVLTR IGVMSLIRKK *F VQEFEHINGY YSMPELILKP CEPVRSALKQ DVAALEAPPT GGNVDKSATT SNSVTPATSA *F APSPAPASEK GEDKDKDSEK EKDKTSAEKS EVKQEQEAEE DKKPGDVKQE NPVEEAAGDT *F KPSDAEVKTE VAKTEPKEET KDPEVKEEPK TEEKEKEKVD DKKPIPPTTV IDDDDDDVMI *F VKEDGELEKP SASSPKDQKA VAAATSAATG ATGKGAEDSL EVLKRKFMFN IADGGFTELH *F TLWLNEEKAA VPGREYEIWH RRHDYWLLAG IVTHGYGRWQ DIQNDIRFAI INEPFKMDVG *F KGNFLEIKNK FLARRFKLLE QALVIEEQLR RAAYLNLAQD PSHPAMSLNA RFAEVECLAE *F SHQHLSKESL AGNKPANAVL HKVLNQLEEL LSDMKSDVSR LPATLARIPP VAQRLQMSER *F SILSRLAATA GNASNAAQLM AQFPAGFQGT TLPAFTSGPA GNFANFRPQF SVPGQLSNNS *F GV *F >O16102 *F SNSMSSKRGA DPDWKTPGKA SKDKRPKTNA KKQKFRDEEY CKVCSDGGDL LCCDSCPSVY *F HRTCLSPPLK SIPKGDWICP RCIPLPGKAE KILSWRWALD RSVELRTSKG EKRREYFIKW *F HGMSYWHCEW IPEGQMLLHH ASMVASFQRR SDMEEPSLEE LDDQDGNLHE RFYRYGIKPE *F WLLVQRVINH SEEPNGGTMY LVKWRELSYN DSSWERESDS IPGLNQAIAL YKKLRSSNKG *F RQRDRPAPTI DLNKKYEDQP VFLKEAGLKL HPFQIEGVSW LRYSWGQGIP TILADEMGLG *F KTIQTVVFLY SLFKEGHCRG PFLISVPLST LTNWERELEL WAPELYCVTY VGGKTARAVI *F RKHEISFEEV TTKTMRENQT QYKFNVMLTS YEFISVDAAF LGCIDWAALV VDEAHRLRSN *F QSKFFRILSK YRIGYKLLLT GTPLQNNLEE LFHLLNFLSS GKFNDLQTFQ AEFTDVSKEE *F QVKRLHEILE PHMLRRLKAD VLKSMPPKSE FIVRVELSSM QKKFYKHILT KNFKALNQKG *F GGRVCSLLNI MMDLRKCCNH PYLFPSAAEE ATISPSGLYE MSSLTKASGK LDLLSKMLKQ *F LKADNHRVLL FSQMTKMLNI LEHFLEGEGY QYDRIDGSIK GDLRQKAIDR FNDPVSEHFV *F FLLSTRAGGL GINLATADTV IIFDSDWNPH NDVQAFSRAH RMGQKKKVMI YRFVTHNSVE *F ERIMQVAKHK MMLTHLVVRP GMGGMTTNFS KDELEDILRF GTEDLFKDGK SEAIHYDDKA *F VADLLDRTNR GIEEKESWAN EYLSSFKVAS YATKEEEEEE ETEIIKQDAE NSDPAYWVKL *F LRHHYEQHQE DVGRSLGKGK RVRKQVNYTD GGVVAADTTR DDSNWQDNGS EYNSEYSAGS *F DEDGGDDDFD DQNGAERKAK RRLERRDDRP LPPLLARVGG NIEVLGFNAR QRKSFLNAIM *F RYGMPPQDAF NSQWLVRDLR GKSERNFKAY VSLFMRHLCE PGADNAETFA DGVPREGLSR *F QHVLTRIGVM SLIRKKVQEF EHINGYYSMP ELILKPCEPV RSALKQDVAA LEAPPTGGNV *F DKSATTSNSV TPATSAAPSP APASEKGEDK DKDSEKEKDK TSAEKSEVKQ EQEAEEDKKP *F GDVKQENPVE EAAGDTKPSD AEVKTEVAKT EPKEETKDPE VKEEPKTEEK EKEKVDDKKP *F IPPTTVIDDD DDDVMIVKED GELEKPSASS PKDQKAVAAA TSAATGATGK GAEDSLEVLK *F RKFMFNIADG GFTELHTLWL NEEKAAVPGR EYEIWHRRHD YWLLAGIVTH GYGRWQDIQN *F DIRFAIINEP FKMDVGKGNF LEIKNKFLAR RFKLLEQALV IEEQLRRAAY LNLAQDPSHP *F AMSLNARFAE VECLAESHQH LSKESLAGNK PANAVLHKVL NQLEELLSDM KSDVSRLPAT *F LARIPPVAQR LQMSERSILS RLAATAGNAS NAAQLMAQFP AGFQGTTLPA FTSGPAGNFA *F NFRPQFSVPG QLSNNSGV *F >AAF49099.1 *F MAHEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKK *F KGKKRKTRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKE *F KESASSGMPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAP *F KLVMLVAAKWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPD *F DIYEEAVEEEEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQD *F ASGASERDSDLEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTK *F IGNKFKKKNKLKKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPEL *F DEPPEGKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPP *F LDTIPDGDWRCPRCSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIK *F WHNMSYWHCEWVPEVQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKD *F KVGMKANDDAEVLEERFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTW *F EEEGDDIQGLRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTP *F PPEKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKT *F IQTVTFLYSLYKEGHCRGPFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIR *F ENELSFEEGAIRGSKVSRLRTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRL *F KSNQSKKSSTSSS *F >AAF49162.1 *F MSSKRGADPDWKTPGKASKDKRPKTNAKKQKFRDEEYCKVCSDGG *F DLLCCDSCPSVYHRTCLSPPLKSIPKGDWICPRCIPLPGKAEKILSWRWALDRSVELRT *F SKGEKRREYFIKWHGMSYWHCEWIPEGQMLLHHASMVASFQRRSDMEEPSLEELDDQDG *F NLHERFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLN *F QAIALYKKLRSSNKGRQRDRPAPTIDLNKKYEDQPVFLKEAGLKLHPFQIEGVSWLRYS *F WGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRGPFLISVPLSTLTNWERELELWAP *F ELYCVTYVGGKTARAVIRKHEISFEEVTTKTMRENQTQYKFNVMLTSYEFISVDAAFLG *F CIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKLLLTGTPLQNNLEELFHLLNFLSSG *F KFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRLKADVLKSMPPKSEFIVRVELSSM *F QKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKCCNHPYLFPSAAEEATISPSGLY *F EMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKMLNVLEHFLEGEGYQYDRIDGS *F IKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATADTVIIFDSDWNPHNDVQAFS *F RAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLVVRPGMGGMTTNFSKDELED *F ILRFGTEDLFKDGKSEAIHYDDKAVADLLDRTNRGIEEKESWANEYLSSFKVASYATKE *F DHEEHDDYNNDAENTDPFYWENLMGKTVAAVPGGRREHHGKGQANPKGNRLLQSISLSK *F SSHPKQYSINVR *F \**E *F O97159 MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRK 60 *F O16102 ------------------------------------------------------------ *F *F *F O97159 TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRARKRKEEKQAAKEKESASSG 120 *F O16102 ------------------------------------------------------------ *F *F *F O97159 MPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAA 180 *F O16102 ------------------------------------------------------------ *F *F *F O97159 KWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEE 240 *F O16102 ------------------------------------------------------------ *F *F *F O97159 EEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSD 300 *F O16102 -------------------------------------------------SNSMSSKRGAD 11 *F . \* :*:*.:* *F *F O97159 LEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKL 360 *F O16102 PDWKTPGKASKDKRPKTNAKKQKFRD---------------------------------- 37 *F ::: : \*.*. :.:* .. \* *F *F O97159 KKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPH 420 *F O16102 ------------------------------------------------------------ *F *F *F O97159 CEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPR 480 *F O16102 ----------------EEYCKVCSDGGDLLCCDSCPSVYHRTCLSPPLKSIPKGDWICP- 80 *F :*:*:**.***:*********.** \**.***.:**.*** \** *F *F O97159 CSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPE 540 *F O16102 -RCIPLPGKAEKILSWRWALDRSVELRTSK------GEKRREYFIKWHGMSYWHCEWIPE 133 *F \* \**.******::**** . : ::. ..: \********.********:** *F *F O97159 VQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEE 600 *F O16102 GQMLLHHASMVASFQRRSDMEEPS-----------------------LEELDDQDGNLHE 170 *F \*: :**. \*: \****: \*****. : :*: \*.* *F *F O97159 RFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDY 660 *F O16102 RFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIAL 230 *F \***: \*:*****:******* :*.**********.*:.*:**.*.*.* \**.*** *F *F O97159 YQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQP 720 *F O16102 YKKLRSSNK-----GRQRDR-------------------------PAPTIDLNKKYEDQP 260 *F \*:.**: . .*.:. \** \**:******* *F *F O97159 AFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRG 780 *F O16102 VFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRG 320 *F .**: :*::***:****:.********** \****************.*****:******* *F *F O97159 PFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRL 840 *F O16102 PFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVIRKHEISFEEVTTKTMREN-- 378 *F \***::******.*****:*****::**:**:*.* :*****::*:**** : : : . *F *F O97159 RTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKL 900 *F O16102 -QTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKL 437 *F \*******:*****:**:*** \**.****.*********:**********..* \*.*** *F *F O97159 LLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRL 960 *F O16102 LLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRL 497 *F \********************** .******:**.**:*************:* \******* *F *F O97159 KTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKC 1020 *F O16102 KADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKC 557 *F \*:****.**.***********:*******.*****::***.*.** \***:******:** *F *F O97159 CNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKM 1080 *F O16102 CNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKM 617 *F \*************** :..****:.*****:*** \***********:*****:******* *F *F O97159 LDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATA 1140 *F O16102 LNILEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATA 677 *F \*:***.***** \*:*:****.*.* \***:****** \* :::******************* *F *F O97159 DTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLV 1200 *F O16102 DTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLV 737 *F \*****:*********:********:** :*********:******: \****:******** *F *F O97159 VRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKE 1260 *F O16102 VRPGMGGMTTNFSKDELEDILRFGTEDLFK-DGKSEAIHYDDKAVADLLDRTNRGIEEKE 796 *F \******* :**:*:**:************ \*.*.***********:************* *F *F O97159 SWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSL 1320 *F O16102 SWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSL 856 *F \************************************************************ *F *F O97159 GKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAE 1380 *F O16102 GKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAE 916 *F \************************************************************ *F *F O97159 RKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLV 1440 *F O16102 RKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLV 976 *F \************************************************************ *F *F O97159 RDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKK 1500 *F O16102 RDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKK 1036 *F \************************************************************ *F *F O97159 VQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSA 1560 *F O16102 VQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSA 1096 *F \************************************************************ *F *F O97159 APSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDT 1620 *F O16102 APSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDT 1156 *F \************************************************************ *F *F O97159 KPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMI 1680 *F O16102 KPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMI 1216 *F \************************************************************ *F *F O97159 VKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELH 1740 *F O16102 VKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELH 1276 *F \************************************************************ *F *F O97159 TLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVG 1800 *F O16102 TLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVG 1336 *F \************************************************************ *F *F O97159 KGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAE 1860 *F O16102 KGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAE 1396 *F \************************************************************ *F *F O97159 SHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSER 1920 *F O16102 SHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSER 1456 *F \************************************************************ *F *F O97159 SILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNS 1980 *F O16102 SILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNS 1516 *F \************************************************************ *F *F O97159 GV 1982 *F O16102 GV 1518 *F \** *F \**F *F *F O97159 MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRK 60 *F AAF49099.1 ---------MAHEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRK 51 *F :************************************************ *F *F O97159 TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRARKRKEEKQAAKEKESASSG 120 *F AAF49099.1 TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSG 111 *F \****************************************.******************* *F *F O97159 MPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAA 180 *F AAF49099.1 MPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAA 171 *F \************************************************************ *F *F O97159 KWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEE 240 *F AAF49099.1 KWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEE 231 *F \************************************************************ *F *F O97159 EEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSD 300 *F AAF49099.1 EEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSD 291 *F \************************************************************ *F *F O97159 LEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKL 360 *F AAF49099.1 LEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKL 351 *F \************************************************************ *F *F O97159 KKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPH 420 *F AAF49099.1 KKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPH 411 *F \************************************************************ *F *F O97159 CEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPR 480 *F AAF49099.1 CEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPR 471 *F \************************************************************ *F *F O97159 CSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPE 540 *F AAF49099.1 CSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPE 531 *F \************************************************************ *F *F O97159 VQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEE 600 *F AAF49099.1 VQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEE 591 *F \************************************************************ *F *F O97159 RFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDY 660 *F AAF49099.1 RFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDY 651 *F \************************************************************ *F *F O97159 YQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQP 720 *F AAF49099.1 YQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQP 711 *F \************************************************************ *F *F O97159 AFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRG 780 *F AAF49099.1 AFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRG 771 *F \************************************************************ *F *F O97159 PFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRL 840 *F AAF49099.1 PFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRL 831 *F \************************************************************ *F *F O97159 RTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKL 900 *F AAF49099.1 RTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKKSSTSSS------- 884 *F \********************************************** .* *F *F O97159 LLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRL 960 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 KTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKC 1020 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 CNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKM 1080 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 LDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATA 1140 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 DTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLV 1200 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 VRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKE 1260 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 SWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSL 1320 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 GKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAE 1380 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 RKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLV 1440 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 RDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKK 1500 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 VQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSA 1560 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 APSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDT 1620 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 KPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMI 1680 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 VKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELH 1740 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 TLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVG 1800 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 KGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAE 1860 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 SHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSER 1920 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 SILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNS 1980 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O97159 GV 1982 *F AAF49099.1 -- *F \**G *F *F O16102 SNSMSSKRGADPDWKTPGKASKDKRPKTNAKKQKFRDEEYCKVCSDGGDLLCCDSCPSVY 60 *F AAF49099.1 -------------------------------------------MAHEEEEAQEDNAPAAE 17 *F :. : \*..*:. *F *F O16102 HRTCLSPPLKSIPKGDWICPRCIPLPGKAEKILSWRWALDRSVELRTSKGEKRREYFIKW 120 *F AAF49099.1 LSNDSDAPLK------------P---NNDEDDD-----YDPEDSRRKKKGKKRK------ 51 *F . ..*** .: \*. \* . . \*..**:**: *F *F O16102 HGMSYWHCEWIPEGQMLLHHASMVASFQRRSDMEEPSLEELDDQDGNLHERFYRYGIKPE 180 *F AAF49099.1 ---------------------TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEY----- 85 *F : .. : \*.. :. . \*. :*.* \*:. .* *F *F O16102 WLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIALYKKLRSSNKG 240 *F AAF49099.1 ----------PSTSKRG----RKRKEEKQAAKEKESASSGMPSVEDVCSAFSVCNVEIEY 131 *F .. .: \* \* :* . .. \* \*..:*.:::. : :. . . : *F *F O16102 RQRDRPAPTIDLNKKYEDQPVFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLG 300 *F AAF49099.1 SEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAAKWREFCESNP---------- 181 *F :.: : \* :. :*:: \** \* : \*: .: :. \* *F *F O16102 KTIQTVVFLYSLFKEGHCRGPFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVI 360 *F AAF49099.1 --HIQQ--------EGGAAG---------S--------------------G-GSAGQARS 201 *F \** . \* : \*.:.:* *F *F O16102 RKHEISFEEVTTKTMRENQTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSN 420 *F AAF49099.1 VTGDEPEEPRSSRSSRNEKPDDIYEEAVEEEEEEEEEEKKP-------------RRKRSG 248 *F . : . \* :::: \*:::.: :: : . \* . : :* \**. *F *F O16102 QSKFFRILSKYRIGYKLLLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEE 480 *F AAF49099.1 RGKKGRRPSGKVPTLKIKLLGK---------------------------------R---- 271 *F :.* \* \* \*: \* \*. *F *F O16102 QVKRLHEILEPHMLRRLKADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKG 540 *F AAF49099.1 -------------K-RDSSDEEQDASGASERDSDLEFERMLQKSDDSADEKEAPVSSKAD 317 *F \* .:* :. . \** :*:. \* :* . \*: . .: . *F *F O16102 GGRVCSLLNIMMDLRKCCNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQ 600 *F AAF49099.1 N--S-------------------APAAQDDGSGAP--------VVRKKAKTKIGNKFKKK 348 *F . \*:* ::.: :* :.: ..* .: .*: \*: *F *F O16102 LKADNHRVLLFSQMTKMLNILEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFV 660 *F AAF49099.1 NKLK------K-TKN--------FPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLV 393 *F \* . . \* \***. :::: \* . : : \* . \* : \*:* *F *F O16102 FLLSTRAGGLGINLATADTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVE 720 *F AAF49099.1 CLEP-------------------------------------------ELD--EP------ 402 *F \* . : *F *F O16102 ERIMQVAKHKMMLTHLVVRPGMGGMTTNFSKDELEDILRFGTEDLFKDGKSEAIHYDDKA 780 *F AAF49099.1 PE------GKWSCPHCEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHT---- 452 *F . \* .* . \* . : .::*: : : \* \* \* \*. ..* \* *F *F O16102 VADLLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKL 840 *F AAF49099.1 ---FCLNPPLDTIPDGDWRCPRCSCPPLTG---KAEK----------------------I 484 *F : .. . . .* \*. ::. \* \*: : *F *F O16102 LRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGS 900 *F AAF49099.1 ITWRWAQRSNDDGPSTSKGSKN------------SNSRVREYFIKWHNMSYWHCEWVPEV 532 *F : :: \*:.:* \* \* .**.: : . .*: .* \* ::.*: . *F *F O16102 DEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIM 960 *F AAF49099.1 QLDVHHP----------LMIRSFQRKYDMEEPPKFEES---------------------- 560 *F : \* . \* ::*: \* \** : . *F *F O16102 RYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSR 1020 *F AAF49099.1 -------------LDEADTR--YKRIQRHKDKVGMKANDDAEVLEERFYKNGVKPEWLIV 605 *F \* \* :* : .: \*: :. . : . : :** \* \* *F *F O16102 QHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNV 1080 *F AAF49099.1 QRVIN------------------------------------------------------- 610 *F \*:*:. *F *F O16102 DKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKP 1140 *F AAF49099.1 ------------HRTAR---------------DGSTMYLVKWRELPYDKSTWEEEGDDIQ 643 *F :* \*... . \* . \*. \* \* \*. *F *F O16102 GDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKP 1200 *F AAF49099.1 G-------LRQAIDYYQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPP 696 *F \* :.:* . : \* .:*.:::..*:..*. : \* : : :*.. \* \* *F *F O16102 IPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLK 1260 *F AAF49099.1 EKPTTDLKKKYEDQPAFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQT 756 *F \*** :... :* . \*. :: . . : : .. .. . : . *F *F O16102 RKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQN 1320 *F AAF49099.1 VTFLYSLYK----EGHCRG--PFLVAVPLSTLVNWEREFELWAPDFYCITYIG----DK- 805 *F .*::.: . \* \* .*** \*.*..: \* \* : *F *F O16102 DIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHP 1380 *F AAF49099.1 DSRAVIRENELSFEEG--------------AIRGSKVSRLRTTQYKFNVLLTSYELISMD 851 *F \* \* .* :: :.:: \* :: : : : \* : . \*. : \* *F *F O16102 AMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPAT 1440 *F AAF49099.1 AACLGSIDWAVLVVDEAHRLKSNQSKKSSTSSS--------------------------- 884 *F \* .*.: \* : \*:*: \*::* ....:. *F *F O16102 LARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFA 1500 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 NFRPQFSVPGQLSNNSGV 1518 *F AAF49099.1 ------------------ *F \**H *F *F O97159 MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRK 60 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRARKRKEEKQAAKEKESASSG 120 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 MPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAA 180 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 KWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEE 240 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 EEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSD 300 *F AAF49162.1 ----------------------------------------------------MSSKRGAD 8 *F :*:*.:* *F *F O97159 LEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKL 360 *F AAF49162.1 PDWKTPGKASKDKRPKTNAKKQKFRD---------------------------------- 34 *F ::: : \*.*. :.:* .. \* *F *F O97159 KKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPH 420 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 CEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPR 480 *F AAF49162.1 ----------------EEYCKVCSDGGDLLCCDSCPSVYHRTCLSPPLKSIPKGDWICP- 77 *F :*:*:**.***:*********.** \**.***.:**.*** \** *F *F O97159 CSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPE 540 *F AAF49162.1 -RCIPLPGKAEKILSWRWALDRSVELRTSK------GEKRREYFIKWHGMSYWHCEWIPE 130 *F \* \**.******::**** . : ::. ..: \********.********:** *F *F O97159 VQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEE 600 *F AAF49162.1 GQMLLHHASMVASFQRRSDMEEPS-----------------------LEELDDQDGNLHE 167 *F \*: :**. \*: \****: \*****. : :*: \*.* *F *F O97159 RFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDY 660 *F AAF49162.1 RFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIAL 227 *F \***: \*:*****:******* :*.**********.*:.*:**.*.*.* \**.*** *F *F O97159 YQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQP 720 *F AAF49162.1 YKKLRSSNK-----GRQRDR-------------------------PAPTIDLNKKYEDQP 257 *F \*:.**: . .*.:. \** \**:******* *F *F O97159 AFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRG 780 *F AAF49162.1 VFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRG 317 *F .**: :*::***:****:.********** \****************.*****:******* *F *F O97159 PFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRL 840 *F AAF49162.1 PFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVIRKHEISFEEVTT---KTMRE 374 *F \***::******.*****:*****::**:**:*.* :*****::*:**** : \*. \* *F *F O97159 RTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKL 900 *F AAF49162.1 NQTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKL 434 *F . \*******:*****:**:*** \**.****.*********:**********..* \*.*** *F *F O97159 LLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRL 960 *F AAF49162.1 LLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRL 494 *F \********************** .******:**.**:*************:* \******* *F *F O97159 KTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKC 1020 *F AAF49162.1 KADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKC 554 *F \*:****.**.***********:*******.*****::***.*.** \***:******:** *F *F O97159 CNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKM 1080 *F AAF49162.1 CNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKM 614 *F \*************** :..****:.*****:*** \***********:*****:******* *F *F O97159 LDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATA 1140 *F AAF49162.1 LNVLEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATA 674 *F \*::**.***** \*:*:****.*.* \***:****** \* :::******************* *F *F O97159 DTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLV 1200 *F AAF49162.1 DTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLV 734 *F \*****:*********:********:** :*********:******: \****:******** *F *F O97159 VRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKE 1260 *F AAF49162.1 VRPGMGGMTTNFSKDELEDILRFGTEDLFK-DGKSEAIHYDDKAVADLLDRTNRGIEEKE 793 *F \******* :**:*:**:************ \*.*.***********:************* *F *F O97159 SWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSL 1320 *F AAF49162.1 SWANEYLSSFKVASYATKEDHEEH--DDYNNDAENTDPFYWENLMGKTVAAVPGGRREHH 851 *F \*******************:.**. : ::****:** \** :*: : . . *F *F O97159 GKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAE 1380 *F AAF49162.1 GKG--------------------------------------------------------- 854 *F \*** *F *F O97159 RKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLV 1440 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 RDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKK 1500 *F AAF49162.1 -----------------------------------------------QANPKGNRLLQSI 867 *F :. \*::. *F *F O97159 VQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSA 1560 *F AAF49162.1 SLSKSSHPKQYSINVR-------------------------------------------- 883 *F . . \**: *F *F O97159 APSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDT 1620 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 KPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMI 1680 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 VKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELH 1740 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 TLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVG 1800 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 KGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAE 1860 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 SHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSER 1920 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 SILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNS 1980 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O97159 GV 1982 *F AAF49162.1 -- *F *F *F *F O16102 SNSMSSKRGADPDWKTPGKASKDKRPKTNAKKQKFRDEEYCKVCSDGGDLLCCDSCPSVY 60 *F AAF49162.1 ---MSSKRGADPDWKTPGKASKDKRPKTNAKKQKFRDEEYCKVCSDGGDLLCCDSCPSVY 57 *F \********************************************************* *F *F O16102 HRTCLSPPLKSIPKGDWICPRCIPLPGKAEKILSWRWALDRSVELRTSKGEKRREYFIKW 120 *F AAF49162.1 HRTCLSPPLKSIPKGDWICPRCIPLPGKAEKILSWRWALDRSVELRTSKGEKRREYFIKW 117 *F \************************************************************ *F *F O16102 HGMSYWHCEWIPEGQMLLHHASMVASFQRRSDMEEPSLEELDDQDGNLHERFYRYGIKPE 180 *F AAF49162.1 HGMSYWHCEWIPEGQMLLHHASMVASFQRRSDMEEPSLEELDDQDGNLHERFYRYGIKPE 177 *F \************************************************************ *F *F O16102 WLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIALYKKLRSSNKG 240 *F AAF49162.1 WLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIALYKKLRSSNKG 237 *F \************************************************************ *F *F O16102 RQRDRPAPTIDLNKKYEDQPVFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLG 300 *F AAF49162.1 RQRDRPAPTIDLNKKYEDQPVFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLG 297 *F \************************************************************ *F *F O16102 KTIQTVVFLYSLFKEGHCRGPFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVI 360 *F AAF49162.1 KTIQTVVFLYSLFKEGHCRGPFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVI 357 *F \************************************************************ *F *F O16102 RKHEISFEEVTTKTMRENQTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSN 420 *F AAF49162.1 RKHEISFEEVTTKTMRENQTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSN 417 *F \************************************************************ *F *F O16102 QSKFFRILSKYRIGYKLLLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEE 480 *F AAF49162.1 QSKFFRILSKYRIGYKLLLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEE 477 *F \************************************************************ *F *F O16102 QVKRLHEILEPHMLRRLKADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKG 540 *F AAF49162.1 QVKRLHEILEPHMLRRLKADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKG 537 *F \************************************************************ *F *F O16102 GGRVCSLLNIMMDLRKCCNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQ 600 *F AAF49162.1 GGRVCSLLNIMMDLRKCCNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQ 597 *F \************************************************************ *F *F O16102 LKADNHRVLLFSQMTKMLNILEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFV 660 *F AAF49162.1 LKADNHRVLLFSQMTKMLNVLEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFV 657 *F \*******************:**************************************** *F *F O16102 FLLSTRAGGLGINLATADTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVE 720 *F AAF49162.1 FLLSTRAGGLGINLATADTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVE 717 *F \************************************************************ *F *F O16102 ERIMQVAKHKMMLTHLVVRPGMGGMTTNFSKDELEDILRFGTEDLFKDGKSEAIHYDDKA 780 *F AAF49162.1 ERIMQVAKHKMMLTHLVVRPGMGGMTTNFSKDELEDILRFGTEDLFKDGKSEAIHYDDKA 777 *F \************************************************************ *F *F O16102 VADLLDRTNRGIEEKESWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKL 840 *F AAF49162.1 VADLLDRTNRGIEEKESWANEYLSSFKVASYATKEDHEEH--DDYNNDAENTDPFYWENL 835 *F \***********************************:.**. : ::****:** \** :* *F *F O16102 LRHHYEQHQEDVGRSLGKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGS 900 *F AAF49162.1 MGKTVAAVPGGRREHHGKG----------------------------------------- 854 *F : : . . \*** *F *F O16102 DEDGGDDDFDDQNGAERKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIM 960 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 RYGMPPQDAFNSQWLVRDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSR 1020 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 QHVLTRIGVMSLIRKKVQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNV 1080 *F AAF49162.1 ------------------------------------------------QANPK---GNRL 863 *F \* \*..: *F *F O16102 DKSATTSNSVTPATSAAPSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKP 1140 *F AAF49162.1 LQSISLSKSSHPKQYSINVR---------------------------------------- 883 *F :* : \*:* \* : *F *F O16102 GDVKQENPVEEAAGDTKPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKP 1200 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 IPPTTVIDDDDDDVMIVKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLK 1260 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 RKFMFNIADGGFTELHTLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQN 1320 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 DIRFAIINEPFKMDVGKGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHP 1380 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 AMSLNARFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPAT 1440 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 LARIPPVAQRLQMSERSILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFA 1500 *F AAF49162.1 ------------------------------------------------------------ *F *F *F O16102 NFRPQFSVPGQLSNNSGV 1518 *F AAF49162.1 ------------------ *F \**J *F *F O16102 ------------------------------------------------------------ *F AAF49162.1 ------------------------------------------------------------ *F O97159 MASEEENDDNFQEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRK 60 *F AAF49099.1 ---------MAHEEEEAQEDNAPAAELSNDSDAPLKPNNDEDDDYDPEDSRRKKKGKKRK 51 *F *F *F O16102 ------------------------------------------------------------ *F AAF49162.1 ------------------------------------------------------------ *F O97159 TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRARKRKEEKQAAKEKESASSG 120 *F AAF49099.1 TRKGEEKGRKKKKRKKNESEEDSDFVQHDEEVEYPSTSKRGRKRKEEKQAAKEKESASSG 111 *F *F *F O16102 ------------------------------------------------------------ *F AAF49162.1 ------------------------------------------------------------ *F O97159 MPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAA 180 *F AAF49099.1 MPSVEDVCSAFSVCNVEIEYSEEELQSLTTYKAFMHHVRPILQKENPKIAAPKLVMLVAA 171 *F *F *F O16102 ------------------------------------------------------------ *F AAF49162.1 ------------------------------------------------------------ *F O97159 KWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEE 240 *F AAF49099.1 KWREFCESNPHIQQEGGAAGSGGSAGQARSVTGDEPEEPRSSRSSRNEKPDDIYEEAVEE 231 *F *F *F O16102 -------------------------------------------------SNSMSSKRGAD 11 *F AAF49162.1 ----------------------------------------------------MSSKRGAD 8 *F O97159 EEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSD 300 *F AAF49099.1 EEEEEEEEKKPRRKRSGRGKKGRRPSGKVPTLKIKLLGKRKRDSSDEEQDASGASERDSD 291 *F :*:*.:* *F *F O16102 PDWKTPGKASKDKRPKTNAKKQKFRD---------------------------------- 37 *F AAF49162.1 PDWKTPGKASKDKRPKTNAKKQKFRD---------------------------------- 34 *F O97159 LEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKL 360 *F AAF49099.1 LEFERMLQKSDDSADEKEAPVSSKADNSAPAAQDDGSGAPVVRKKAKTKIGNKFKKKNKL 351 *F ::: : \*.*. :.:* .. \* *F *F O16102 ------------------------------------------------------------ *F AAF49162.1 ------------------------------------------------------------ *F O97159 KKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPH 420 *F AAF49099.1 KKTKNFPEGEDGEHEHQDYCEVCQQGGEIILCDTCPRAYHLVCLEPELDEPPEGKWSCPH 411 *F *F *F O16102 ----------------EEYCKVCSDGGDLLCCDSCPSVYHRTCLSPPLKSIPKGDWICP- 80 *F AAF49162.1 ----------------EEYCKVCSDGGDLLCCDSCPSVYHRTCLSPPLKSIPKGDWICP- 77 *F O97159 CEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPR 480 *F AAF49099.1 CEADGGAAEEEDDDEHQEFCRVCKDGGELLCCDSCPSAYHTFCLNPPLDTIPDGDWRCPR 471 *F :*:*:**.***:*********.** \**.***.:**.*** \** *F *F O16102 -RCIPLPGKAEKILSWRWALDRSVELRTSK------GEKRREYFIKWHGMSYWHCEWIPE 133 *F AAF49162.1 -RCIPLPGKAEKILSWRWALDRSVELRTSK------GEKRREYFIKWHGMSYWHCEWIPE 130 *F O97159 CSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPE 540 *F AAF49099.1 CSCPPLTGKAEKIITWRWAQRSNDDGPSTSKGSKNSNSRVREYFIKWHNMSYWHCEWVPE 531 *F \* \**.******::**** . : ::. ..: \********.********:** *F *F O16102 GQMLLHHASMVASFQRRSDMEEPS-----------------------LEELDDQDGNLHE 170 *F AAF49162.1 GQMLLHHASMVASFQRRSDMEEPS-----------------------LEELDDQDGNLHE 167 *F O97159 VQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEE 600 *F AAF49099.1 VQLDVHHPLMIRSFQRKYDMEEPPKFEESLDEADTRYKRIQRHKDKVGMKANDDAEVLEE 591 *F \*: :**. \*: \****: \*****. : :*: \*.* *F *F O16102 RFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIAL 230 *F AAF49162.1 RFYRYGIKPEWLLVQRVINHSEEPNGGTMYLVKWRELSYNDSSWERESDSIPGLNQAIAL 227 *F O97159 RFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDY 660 *F AAF49099.1 RFYKNGVKPEWLIVQRVINHRTARDGSTMYLVKWRELPYDKSTWEEEGDDIQGLRQAIDY 651 *F \***: \*:*****:******* :*.**********.*:.*:**.*.*.* \**.*** *F *F O16102 YKKLRSSNKGRQRDR------------------------------PAPTIDLNKKYEDQP 260 *F AAF49162.1 YKKLRSSNKGRQRDR------------------------------PAPTIDLNKKYEDQP 257 *F O97159 YQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQP 720 *F AAF49099.1 YQDLRAVCTSETTQSRSKKSKKGRKSKLKVEDDEDRPVKHYTPPPEKPTTDLKKKYEDQP 711 *F \*:.**: ... : \** \**:******* *F *F O16102 VFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRG 320 *F AAF49162.1 VFLKEAGLKLHPFQIEGVSWLRYSWGQGIPTILADEMGLGKTIQTVVFLYSLFKEGHCRG 317 *F O97159 AFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRG 780 *F AAF49099.1 AFLEGTGMQLHPYQIEGINWLRYSWGQGIDTILADEMGLGKTIQTVTFLYSLYKEGHCRG 771 *F .**: :*::***:****:.********** \****************.*****:******* *F *F O16102 PFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVIRKHEISFEEVTT---KTMRE 377 *F AAF49162.1 PFLISVPLSTLTNWERELELWAPELYCVTYVGGKTARAVIRKHEISFEEVTT---KTMRE 374 *F O97159 PFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRL 840 *F AAF49099.1 PFLVAVPLSTLVNWEREFELWAPDFYCITYIGDKDSRAVIRENELSFEEGAIRGSKVSRL 831 *F \***::******.*****:*****::**:**:*.* :*****::*:**** : \*. \* *F *F O16102 NQTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKL 437 *F AAF49162.1 NQTQYKFNVMLTSYEFISVDAAFLGCIDWAALVVDEAHRLRSNQSKFFRILSKYRIGYKL 434 *F O97159 RTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKFFRILNSYTIAYKL 900 *F AAF49099.1 RTTQYKFNVLLTSYELISMDAACLGSIDWAVLVVDEAHRLKSNQSKKSSTSSS------- 884 *F . \*******:*****:**:*** \**.****.*********:***** .. *F *F O16102 LLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRL 497 *F AAF49162.1 LLTGTPLQNNLEELFHLLNFLSSGKFNDLQTFQAEFTDVSKEEQVKRLHEILEPHMLRRL 494 *F O97159 LLTGTPLQNNLEELFHLLNFLSRDKFNDLQAFQGEFADVSKEEQVKRLHEMLGPHMLRRL 960 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 KADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKC 557 *F AAF49162.1 KADVLKSMPPKSEFIVRVELSSMQKKFYKHILTKNFKALNQKGGGRVCSLLNIMMDLRKC 554 *F O97159 KTDVLKNMPSKSEFIVRVELSAMQKKFYKFILTKNYEALNSKSGGGSCSLINIMMDLKKC 1020 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 CNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKM 617 *F AAF49162.1 CNHPYLFPSAAEEATISPSGLYEMSSLTKASGKLDLLSKMLKQLKADNHRVLLFSQMTKM 614 *F O97159 CNHPYLFPSAAEEATTAAGGLYEINSLTKAAGKLVLLSKMLKQLKAQNHRVLIFSQMTKM 1080 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 LNILEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATA 677 *F AAF49162.1 LNVLEHFLEGEGYQYDRIDGSIKGDLRQKAIDRFNDPVSEHFVFLLSTRAGGLGINLATA 674 *F O97159 LDILEDFLEGEQYKYERIDGGITGTLRQEAIDRFNAPGAQQFVFLLSTRAGGLGINLATA 1140 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 DTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLV 737 *F AAF49162.1 DTVIIFDSDWNPHNDVQAFSRAHRMGQKKKVMIYRFVTHNSVEERIMQVAKHKMMLTHLV 734 *F O97159 DTVIIYDSDWNPHNDIQAFSRAHRIGQANKVMIYRFVTRNSVEERVTQVAKRKMMLTHLV 1200 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 VRPGMGGMTTNFSKDELEDILRFGTEDLFK-DGKSEAIHYDDKAVADLLDRTNRGIEEKE 796 *F AAF49162.1 VRPGMGGMTTNFSKDELEDILRFGTEDLFK-DGKSEAIHYDDKAVADLLDRTNRGIEEKE 793 *F O97159 VRPGMGGKGANFTKQELDDILRFGTEDLFKEDDKEEAIHYDDKAVAELLDRTNRGIEEKE 1260 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 SWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSL 856 *F AAF49162.1 SWANEYLSSFKVASYATKEDHEEH--DDYNNDAENTDPFYWENLMGKTVAAVPGGRREHH 851 *F O97159 SWANEYLSSFKVASYATKEEEEEEETEIIKQDAENSDPAYWVKLLRHHYEQHQEDVGRSL 1320 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 GKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAE 916 *F AAF49162.1 GKG--------------------------------------------------------- 854 *F O97159 GKGKRVRKQVNYTDGGVVAADTTRDDSNWQDNGSEYNSEYSAGSDEDGGDDDFDDQNGAE 1380 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 RKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLV 976 *F AAF49162.1 ------------------------------------------------------------ *F O97159 RKAKRRLERRDDRPLPPLLARVGGNIEVLGFNARQRKSFLNAIMRYGMPPQDAFNSQWLV 1440 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 RDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKK 1036 *F AAF49162.1 ------------------------------------------------------------ *F O97159 RDLRGKSERNFKAYVSLFMRHLCEPGADNAETFADGVPREGLSRQHVLTRIGVMSLIRKK 1500 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 VQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSA 1096 *F AAF49162.1 -----------------------------------QANPKGNRLLQSISLSKSSHPKQYS 879 *F O97159 VQEFEHINGYYSMPELILKPCEPVRSALKQDVAALEAPPTGGNVDKSATTSNSVTPATSA 1560 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 APSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDT 1156 *F AAF49162.1 INVR-------------------------------------------------------- 883 *F O97159 APSPAPASEKGEDKDKDSEKEKDKTSAEKSEVKQEQEAEEDKKPGDVKQENPVEEAAGDT 1620 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 KPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMI 1216 *F AAF49162.1 ------------------------------------------------------------ *F O97159 KPSDAEVKTEVAKTEPKEETKDPEVKEEPKTEEKEKEKVDDKKPIPPTTVIDDDDDDVMI 1680 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 VKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELH 1276 *F AAF49162.1 ------------------------------------------------------------ *F O97159 VKEDGELEKPSASSPKDQKAVAAATSAATGATGKGAEDSLEVLKRKFMFNIADGGFTELH 1740 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 TLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVG 1336 *F AAF49162.1 ------------------------------------------------------------ *F O97159 TLWLNEEKAAVPGREYEIWHRRHDYWLLAGIVTHGYGRWQDIQNDIRFAIINEPFKMDVG 1800 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 KGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAE 1396 *F AAF49162.1 ------------------------------------------------------------ *F O97159 KGNFLEIKNKFLARRFKLLEQALVIEEQLRRAAYLNLAQDPSHPAMSLNARFAEVECLAE 1860 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 SHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSER 1456 *F AAF49162.1 ------------------------------------------------------------ *F O97159 SHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKSDVSRLPATLARIPPVAQRLQMSER 1920 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 SILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNS 1516 *F AAF49162.1 ------------------------------------------------------------ *F O97159 SILSRLAATAGNASNAAQLMAQFPAGFQGTTLPAFTSGPAGNFANFRPQFSVPGQLSNNS 1980 *F AAF49099.1 ------------------------------------------------------------ *F *F *F O16102 GV 1518 *F AAF49162.1 -- *F O97159 GV 1982 *F AAF49099.1 -- # *U FBrf0128114 *a White *b K. *t 2000.5.3 *T personal communication to FlyBase *u *F Date: Wed, 3 May 2000 10:15:22 -0700 *F To: Chihiro Yamada *F From: Kalpana White *F Subject: Re: FlyBase Query (cy548) *F > *F > *F >I am currently curating your paper for FlyBase: *F >Koushika et al., 2000, Molec. Cell. Biol. 20(5): 1836--1845 *F > *F >I have a few questions I was hoping you could answer for me. *F > *F ... *F > *F > *F >Later in your materials and methods you mention a construct you call *F >P{w+=elav)DmORF2. Is this the same rescue construct as mentioned *F >above? You cite: *F > *F >Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F YES- an insert on the second chromosome of P{elavDMORF} *F > *F >in which P{elavDmORF} and P{elavDmORF-120} are both mentioned. *F > *F >elavDmORF *F > Koushika et al., 1996, Curr. Biol. 6(12): 1634--1641 *F > Samson, 1998, Genetics 150(2): 723--733 *F > Yannoni and White, 1999, J. Cell Sci. 112(24): 4501--4512 *F > Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F > Yao et al., 1993, J. Neurobiol. 24(6): 723--739 *F > Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F > P{elavDmORF} *F > Provides 72% rescue of lethality of @elav5@. *F > Genomic DNA covering 7.5kb of the @elav@ locus, including the ORF, *F > fused to 800bp of @&agr;Tub84B@ trailer sequences. *F > *F > *F >elavDmORF-120 *F > Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F > P{elavDmORF-120} *F > Provides comparable rescue of lethality of @elav5@ to that *F > provided by @elavDmORF@. *F > Deletion of 120 nucleotides (encoding amino acids 35 to 74) *F > within the region corresponding to the homopolymeric *F > alanine/glutamine-rich domain. *F > *F > *F >Is P{w+=elav)DmORF2 one of these? If so does the '2' indicate a specific *F >insertion? *F > *F > *F >Thanks in advance for helping me sort this out, I hope to hear from you soon. *F > *F >Best wishes, *F > *F >Chihiro *F Kalpana White *F Biology Department MS008 *F Brandeis University *F P.O.Box 549110 *F Waltham MA 02454-9110 *F Ph \# 781 736 3175 *F FAX \# 781 736 3107 *F ============================================================================== *F To: white@binah.cc.brandeis.EDU *F Subject: FlyBase Query (cy548) *F From: Chihiro Yamada *F Date: Wed, 3 May 2000 12:02:27 +0100 *F Dear Dr White, *F I am currently curating your paper for FlyBase: *F Koushika et al., 2000, Molec. Cell. Biol. 20(5): 1836--1845 *F I have a few questions I was hoping you could answer for me. *F __elav genomic rescue constructs__ *F elav genomic rescue constructs are mentioned in a few different places *F in your paper, and it is not clear to me how many different constructs *F have been used, or whether different names have been given to the same *F construct in different places. *F In your materials and methods you mention an elav genomic rescue *F construct called P{w+=elav+} which was used to make the elavedr *F allele. You cite: *F Koushika et al., 1996, Curr. Biol. 6(12): 1634--1641 *F In which the rescue fragment P{elavDmORF} is mentioned. Is this the *F construct that is present in the elavedr allele? *F Later in your materials and methods you mention a construct you call *F P{w+=elav)DmORF2. Is this the same rescue construct as mentioned *F above? You cite: *F Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F in which P{elavDmORF} and P{elavDmORF-120} are both mentioned. *F elavDmORF *F Koushika et al., 1996, Curr. Biol. 6(12): 1634--1641 *F Samson, 1998, Genetics 150(2): 723--733 *F Yannoni and White, 1999, J. Cell Sci. 112(24): 4501--4512 *F Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F Yao et al., 1993, J. Neurobiol. 24(6): 723--739 *F Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F P{elavDmORF} *F Provides 72% rescue of lethality of @elav5@. *F Genomic DNA covering 7.5kb of the @elav@ locus, including the ORF, *F fused to 800bp of @&agr;Tub84B@ trailer sequences. *F elavDmORF-120 *F Yao and White, 1991, Molec. Cell. Biol. 11(6): 2994--3000 *F P{elavDmORF-120} *F Provides comparable rescue of lethality of @elav5@ to that *F provided by @elavDmORF@. *F Deletion of 120 nucleotides (encoding amino acids 35 to 74) *F within the region corresponding to the homopolymeric *F alanine/glutamine-rich domain. *F Is P{w+=elav)DmORF2 one of these? If so does the '2' indicate a specific *F insertion? *F Thanks in advance for helping me sort this out, I hope to hear from you soon. *F Best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0128126 *a Lammel *b U. *t 2000.5.19 *T personal communication to FlyBase *u *F Date: Fri, 19 May 2000 09:30:57 +0200 *F To: Chihiro Yamada *F From: uwe lammel *F Subject: Re: FlyBase Query (cy579) *F Dear Dr Yamada, *F the UAS-brk described in section 2.4 was generated by us with the described *F cDNA clone GM06062 from the BDGP and was full length cloned in the pUAST *F using NotI and KpnI. *F Best regards *F Uwe *F >Dear Dr Lammel, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Lammel et al., 2000, Mech. Dev.92(2): 179--191 *F > *F >I have a quick question I was hoping you could answer for me. *F > *F >In section 2.4 you mention a UAS-brk construct. I was wondering, did *F >you use the Jazwinska construct used in: *F > *F >Jazwinska et al., 1999, Cell 96(4): 563--573 *F >Jazwinska et al., 1999, Development 126(15): 3323--3334 *F > *F >brkScer\UAS.cJa *F >P{UAS-brk.J} *F >UAS-brk *F >Expression of brk cDNA is governed by UAS regulatory sequences. *F > *F >If this is not the case, could you give me some more information on the *F >allele used? Did you make it yourselves? If so could you give me some *F >more molecular details about this construct? *F > *F >I hope to hear from you soon. *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- # *U FBrf0128159 *a Inoue *b Y. *t 2000.5.9 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Apr 11 13:20:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Apr 2000 13:20:53 +0100 *F To: yhinoue@drochan.bio.kit.ac.jp *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 11 Apr 2000 13:25:26 +0100 *F Content-Length: 2005 *F Dear Dr. Inoue, *F I am curating your paper for FlyBase: *F Inoue et al., 2000, J. Cell Biol. 149(1): 153--165 *F and I have a question. *F 1. orbit1 mutant ( = superscript). *F You state that this mutant is one of the P-element mutations from the *F paper: *F Deak et al., 1997, Genetics 147(4): 1697--1722 *F I would be grateful if you could tell me the line number for this *F mutation, e.g. 0143/27, 0661/21 as we almost certainly have this line *F already in FlyBase, and if I know which line it is I can avoid *F duplication in the database. *F . *F . *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From yhinoue@drochan.bio.kit.ac.jp Tue May 09 13:14:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 13:14:32 +0100 *F X-Mailer: Macintosh Eudora Pro Version 2.1.3-J *F Mime-Version: 1.0 *F Date: Tue, 9 May 2000 21:29:37 +0900 *F To: Gillian Millburn (Genetics) *F From: yhinoue@drochan.bio.kit.ac.jp (Y. Inoue) *F Subject: Re: FlyBase query *F Dear Gillian, *F Thank you for your E-mail. I am sorry that I was not able to reply sooner to *F your first mail. *F I would like to answer your question. *F 1. orbit1 mutant ( = superscript). *F It corresponds to a line number 0686/07 in the P element collection described *F in Deak et al., 1997, Genetics 147(4): 1697--1722. *F . *F . *F Best wishes, *F Yoshi *F \----------------------------------- *F Yoshihiro H. Inoue PhD *F Drosophila Genetic Resource Center *F Kyoto Institute of Technology *F Matsugasaki, Sakyo-ku, *F Kyoto 606-8585, Japan *F Tel: +819018635827(mobile phone) *F Telfax: +81(75)724-7710 *F E-mail: yhinoue@drochan.bio.kit.ac.jp *F \----------------------------------- # *U FBrf0128160 *a Laverty *b T. *t 2000.4.13 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Apr 13 20:58:55 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GMR-eagle} insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{GMR-eagle} insertion *F The following information accompanied stocks from Todd Laverty, UC Berkeley *F (3/00). *F P{GMR-eagle}10 is a homozygous lethal insertion on the third chromosome. *F The construct and its transformation were described in Hay et *F al. 1997 PNAS 94(10): 5195--5200 FBrf0093501. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128161 *a Carthew *b R. *t 2000.4.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Apr 14 22:03:30 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: sina3 and sinaA16 *F From: Kevin Cook, Bloomington Stock Center *F Subject: sina3 and sinaA16 *F Richard Carthew confirmed in correspondence (4/14/00) that sinaA16 is a *F synonym for sina3. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128162 *a Gelbart *b W. *t 2000.4.17 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Mon Apr 17 12:35:19 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Smox - Sad merge - How Sad *F Cc: debbie_andrew@qmail.bs.jhu.edu, gelbart@morgan.harvard.edu *F FB-Cambridge folks, *F Sad (aka Sisters against dpp; FBgn0024304) and Smox (aka Smad on X; *F FBgn0025800) should be merged. Both names come from the Andrew *F lab. While Sad has historic precedence based on a pc and on a *F fly meeting abstract, I spoke with Debbie before she published her *F paper that included Smox and we agreed that Sad was inappropriate *F because (a) there was no evidence of a genetic interaction of any *F kind with dpp and hence, Sad would be misleading, and (b) the *F sequence similarity of the gene to vertebrate Smad2 suggests that *F it is more likely to be involved in the fly's activin pathway(s) *F than in its dpp/BMP pathways. *F Hence, I strongly feel that Smox should be the merged name. I *F have cc'd Debbie so that she can confirm/disagree. *F Bill # *U FBrf0128163 *a Laverty *b T. *t 2000.4.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 16:55:21 2000 *F To: Rachel Drysdale (Genetics) *F Subject: PTP-ER aberrations *F From: Kevin Cook, Bloomington Stock Center *F Subject: PTP-ER aberrations *F The following information accompanied stocks sent by Todd Laverty, UC *F Berkeley (3/00). *F 1. In(2)XE-2776 has breakpoints in 2R centric heterochromatin and 57F *F (within PTP-ER) , so it should be designated In(2R)XE-2776. *F 2. The PTP-ERXE-2900 mutation is a deletion of 57F2;58A1 rather than a *F point mutation in the PTP-ER gene. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128164 *a Chang *b H. *t 2000.4.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 17:19:07 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Sev-phyl} construct and insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{Sev-phyl} construct and insertion *F In reply to an inquiry about the visible marker and insertion site of the *F P{Sev-phyl} construct described in Chang et al., 1995 Cell 80(3): *F 463--472 (FBrf0079934), Henry Chang, Yale University wrote (4/17/00): *F 'Sev-phyl was constructed by fusing the phyllopod cDNA (generated by PCR) *F to the Sev enhancer/Sev promoter (described by David Bowtell). The *F transformation vector was pDM30 which carries a ry+ marker. I think the *F original insertion was on the third chromosome. So the genotype should be: *F \+; +; P{sev-phyl, ry+}, ry506/P{sev-phyl, ry+}, ry506' *F . *F . *F . *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128165 *a Laverty *b T. *t 2000.4.18 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 17:19:07 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Sev-phyl} construct and insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{Sev-phyl} construct and insertion *F . *F . *F . *F The insertion, P{ry+t7.2=Sev-phyl}HC1, is homozygous viable and fertile *F (Todd Laverty, UC Berkeley, 3/00). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128166 *a Laverty *b T. *t 2000.4.19 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 17:30:08 2000 *F To: Rachel Drysdale (Genetics) *F Subject: P{w+mC=GMR-phyl} insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{w+mC=GMR-phyl} insertion *F The following information accompanied stocks sent by Todd Laverty, UC *F Berkeley (3/00). *F An insertion of P{w+mC=GMR-phyl} generated by the work described in Chang *F et al. Cell 1995 80(3):463--472, 1995 (FBrf0079934) is a lethal insertion *F on the third chromosome. *F (Note that the visible marker is w+mC because the construct was made *F using pP{GMR}. See FBrf0079934.) *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128167 *a Laverty *b T. *t 2000.4.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 17:46:04 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Rhodopsin construct insertions *F From: Kevin Cook, Bloomington Stock Center *F Subject: Rhodopsin construct insertions *F The following information accompanied stocks sent by Todd Laverty, UC *F Berkeley (3/00). *F P{Rh3.343,lacZ}1 and P{Rh4.1900lacZ}11 are viable and fertile X chromosome *F insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128168 *a Laverty *b T. *t 2000.4.21 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 18:12:16 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GMR-DIAP1}1 and P{GMR-DIAP2}BH1 insertions *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{GMR-DIAP1}1 and P{GMR-DIAP2}BH1 insertions *F The following information accompanied stocks from Todd laverty, UC Berkeley *F (3/00). *F P{GMR-DIAP1}1 is a homozygous viable and fertile second chromosome insertion. *F P{GMR-DIAP2}BH1 is a homozygous viable and fertile third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128169 *a Laverty *b T. *t 2000.4.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 19:04:39 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GMRP35}X-1 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{GMRP35}X-1 insertion *F The following information accompanied stocks sent by Todd Laverty, UC *F Berkeley (3/00). *F P{GMRP35}X-1 is a homozygous viable and fertile X chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128170 *a Baum *b B. *t 2000.4.17 *T personal communication to FlyBase *u *F From bbaum@rascal.med.harvard.edu Mon Apr 17 19:57:47 2000 *F To: flybase-help@morgan.harvard.edu *F I wanted to let you know that the gene capulet and ACAP are one and the *F same. Also Jessica Treisman has a paper in press in Cell describing the *F isolation and characterisation of the gene, while my paper with Norbert *F Perrimon is currently under review. *F I hope this is helpful. *F Yours *F Buzz Baum *F Buzz Baum, *F Dept. of Genetics, *F Harvard Med. School, HHMI, *F 200 Longwood Ave., *F Boston, *F MA 02115. *F tel:617 432 7548 *F fax:617 432 7688 # *U FBrf0128171 *a Laverty *b T. *t 2000.4.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 17 21:52:57 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{sevroI}1 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{sevroI}1 insertion *F The following information accompanied stocks from Todd Laverty, UC Berkeley *F (3/00). *F P{sevroI}1 is a homozygous viable and fertile second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128172 *a Laverty *b T. *t 2000.4.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 18 20:55:55 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{hs.sev-PTP-ER.K}101 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{hs.sev-PTP-ER.K}101 insertion *F The following information accompanied stocks from Todd Laverty, UC Berkeley *F (3/00). *F P{hs.sev-PTP-ER.K}101 is a homozygous and hemizygous viable and fertile X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128174 *a Christensen *b A.C. *t 2000.4.27 *T personal communication to FlyBase *u *F From acc@biocomp.unl.edu Thu Apr 27 23:39:54 2000 *F To: flybase-help@morgan.harvard.edu *F Subject: annotation *F BcDNA:GH02976 and BEST:CK00178 are the same thing. # *U FBrf0128175 *a Muskavitch *b M. *t 2000.5.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed May 03 16:56:06 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Su(H).myc}SK1 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{Su(H).myc}l(2)SK1SK1 insertion *F The following information accompanied stocks from Marc Muskavitch, Indiana *F University (4/00). *F P{w+mC=Su(H).myc}l(2)SK1SK1 is a lethal second chromosome insertion. *F The construction and transformation of P{Su(H).myc} were described in Kidd *F et al. Genes Dev. 1998 12(23):3728--3740 (FBrf0105862). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128176 *a Bloomington Drosophila Stock Center *b ?. *t 2000.5.3 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Wed May 03 19:06:46 2000 *F Subject: rasE6 is a synonym for rasl1 *F To: flybase-updates@morgan.harvard.edu *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Dated: 3 May 2000 *F Information communicated: *F The allele rasl1 is known to many fly people as rasE6. Please add rasE6 as a *F synonym for rasl1. # *U FBrf0128177 *a Georgiev *b P. *t 2000.5.3 *T personal communication to FlyBase *u *F From gpg@mx.ibg.relarn.ru Wed May 03 08:53:14 2000 *F To: 'Michael Ashburner \(Genetics\)' *F Subject: X element *F Dear Dr. Ashburner, *F Sorry for the delay with answer. The X element described in Genetica *F 1992 was not a real mobile element. In Genetics 146:583-594 (1997) we *F described the structure of such hybrid P elements inserted in the yellow *F gene. The hybrid element was flanked by two identical copies of P elements. *F The central part of the inserted element consists of genomic sequences *F originating from different regions of the X chromosome. This chimeric *F element can transpose only by subsequent inversion-reinversions. Thus, such *F elements were not real mobile elements and we used X element as a temporal *F name. *F With the best wishes, *F Pavel Georgiev # *U FBrf0128178 *a O'Hare *b K. *t 2000.5.1 *T personal communication to FlyBase *u *F >From k.ohare@ic.ac.uk Mon May 01 15:39:12 2000 *F To: Michael Ashburner (Genetics) *F Subject: Is pogo complete ? *F I'm attaching the graphical output from searching the genomic sequence with *F the longest pogo sequence (X59837). It looks (satisfyingly) as we had found - the *F ~2.1 kb are the largest with a bunch of elements between 1.1 and 1.5 kb *F having different internal deletions, and lots and lots of tiny elements *F around 0.2 kb. *F I've not looked in detail at the sequence alignments - we did find some *F polymorphisms between individual elements anyway. The apparent *F discontinuity around 250-300 is not real - just BLAST taking out a region *F of relatively simple sequence (mostly a run of T). *F So I guess the 2.1 kb sequence can probably be called complete. David *F Finnegan did take these clones and express the encoded protein and showed *F that it bound the ends of pogo: *F Wang H, Hartswood E, Finnegan DJ (1999) Pogo transposase contains a *F putative helix-turn-helix DNA binding domain that recognises a 12 bp *F sequence within the terminal inverted repeats. Nucleic Acids Res 27:455-61. *F Kevin # *U FBrf0128179 *a O'Hare *b K. *t 2000.5.2 *T personal communication to FlyBase *u *F >From k.ohare@ic.ac.uk Mon May 01 13:07:24 2000 *F To: Michael Ashburner (Genetics) *F From: Kevin O'Hare *F Subject: a complete F *F Michael, *F I've made up a dummy genbank file for the F element (attached as a text *F file). I chose this one from the 4 that were 'complete' as it had the most *F obvious flanking duplication. If you need any more information, let me know. *F Kevin LOCUS FELEMENT 4708 bp DNA INV 01-MAY-2000 *F DEFINITION Complete F transposable element deduced from Drosophila *F melanogaster genomic DNA sequence. Includes gag-related and reverse *F transcriptase genes. *F ACCESSION FELEMENT *F KEYWORDS . *F SOURCE fruit fly. *F ORGANISM Drosophila melanogaster *F Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; *F Drosophilidae; Drosophila. *F REFERENCE 1 (bases 1 to 4708) *F AUTHORS O'Hare,K. *F TITLE F element from Drosophila melanogaster *F JOURNAL Unpublished *F REFERENCE 2 (bases 1 to 4708) *F AUTHORS O'Hare,K. *F TITLE Direct Submission *F JOURNAL Submitted (01-MAY-2000) Department of Biochemistry, Imperial *F College of Science, Technology & Medicine, London SW7 2AZ, UK *F FEATURES Location/Qualifiers *F source 1..4708 *F /organism='Drosophila melanogaster' *F /note='This sequence is from AC005198. Other complete *F elements exist in AC006414, AC006302, and AC007888. This *F extends at the 5' end the sequence of the FW element in *F M17214' *F CDS 192..1880 *F /note='From translation of DNA sequence' *F /codon_start=1 *F /product='gag-related' *F /translation='MSQNDTRAQRQREHDERRLSIQRNNAYFSYVSPTIPNADIERSI *F THSPGNLLLPTNQERARSCSPALLAPTEAPLPPTTTAGEGPAARSASSSAAPAHGLTK *F SAKAKPLAINGTAALPAKQNENVNKKAGSTWQTGMDRYITIKRKLSPENSDLGNKPKN *F TRDNSTLIKNVAPANTNRFALLVDTAEDVPLGSVDIEPKKTKPPPIYIREKSTSRLVN *F TLIGLIGKDSFHIIPLVRGTINEIKLQTKTEDDYRKVTNYFTAQKIGFYTYQLKSSKG *F LQVVLKGIESDVTPEEITEALKEKGFYAKNVFNIKNRNRQPQPLFKIELEPENKPPRK *F NEVHPIYKLQLLLHRRITVEEPHKRNAPVQCTNCQEYGHTRSYCTLRPVCVVCGDLHD *F SKQCQINKENACEKKCNNCGGNHTANYRGCPIYKELKIRLHKRMNTARAHQGSATLIP *F SETNPEVIFSKAASFAPWPTFNTNKTTFANVLKSGMTPPTQNSRTPHEVHTKLDTQQN *F YHPAAQQETKTEAMMQALQQSMMEFMTFMKTTIQDMMRNQNLLIQMLVAQQSNK' *F CDS 1880..4561 *F /note='From translation of DNA sequence' *F /codon_start=1 *F /product='reverse transcriptase' *F /translation='MATLRIATWNANGVSQRKLELAQFLHEKHIDVMLLSETHLTSKY *F NFQIRDYHFYGTNHPDGKAHGGTAILIRNRMKHHFYKEFAENHLQATSINIQLDDNTL *F LTLAAVYCPPRFTVLEAQFLDFFQALGPHFIAAGDYNAKHTHWGSRLVNPKGKQLYKT *F IIKATNKLDHVSPGSPTYWPSDLNKLPDLIDFAVTKNISRSLVKAECLPDLSSDHSPV *F LIHLRRYAENVKPPTRLTSSKTNWLRYKKYISSHIELSPKLNTESDIESCTCALQSIL *F TAAALTATPKITNNTINSKKTNVQIEQLVHVKRRLRREWQSSRSPTAKQKLKVATRKL *F ANALKQEEDDDQRRYIEQLTPTGTKQKSLWRAHSTLRPPTETVLPIRNSSGGWARSDE *F DRANTFAAHLQNVFTPNQATSTFALPSYPVNRHQQHTPIVFRPKEITKIIKDNLSPKK *F SPGYDLITPEMIIQLPHSAVRYITKLFNAITKLGYFPQRWKMMKIIMIPKPGKNHTVA *F SSYRPISLLSCISKLFEKCLLIRLNQHLIYHNIIPAHQFGFRESHGTIEQVNRITTEI *F RTAFEYREYCTAVFLDVSQAFDKVWLDGLMFKIKTSLPESTHKLLKSYLYDRKFAVRC *F NTATSTVHTIEAGVPQGSVLGPTLYLIYTADIPTNSRLTVSTFADDTAILSRSRSPIQ *F ATAQLALYLIDIEKWLSDWRIKVNEQKCKHVTFTLNRQDCPPLLLNSIPLPKADEVTY *F LGVHLDRRLTWRRHIEAKKTQLKLKANNLHWLINSGSPLSLDHKVLLYNSILKPIWTY *F GSQLWGNASNSNIDIIQRAQSKILRTITGAPWYVRSENIQRDLNIPSVTNAITELKEK *F YHSKLHTHPNHLARGLIQLSSRSRLRRKDLPTQRINY' *F BASE COUNT 1618 a 1242 c 857 g 991 t *F ORIGIN *F 1 aatcaattaa tcaattcgat cgccgacgtg tgaagacgtt tttatcgtgc tccgcacaaa *F 61 atcggttgtt ttgagtgaag tgaacgccaa ataaaataaa ctaaataaaa aatctgaaag *F 121 cgaaagagac gctctatgcg atgcaagatc gcttaaatac atagtgaatt gttatcttaa *F 181 ataataaaac tatgagtcag aatgacactc gcgcccagcg tcagcgcgag catgacgaac *F 241 gccgactctc aattcagcgc aacaacgcgt acttctccta cgtctcaccg acaatcccaa *F 301 acgcagacat cgagcggtca ataacccata gcccaggaaa ccttcttcta ccaacaaatc *F 361 aagaaagagc gcgctcctgc tctcccgctc tattggctcc gacagaagcc ccgctacctc *F 421 caacaacaac agctggagag ggaccggcag cccgctctgc ctcgtcatcg gctgcacccg *F 481 ctcacggtct gactaagtca gcgaaagcaa aaccgctagc aataaacggt actgctgcac *F 541 tgccagcaaa acaaaacgaa aacgtaaaca aaaaagctgg gtcgacctgg cagactggaa *F 601 tggaccgcta cattacaata aagcgaaagc tcagcccgga aaattcagat ttgggaaaca *F 661 agccgaaaaa tacacgcgat aactctacct tgatcaaaaa tgtagcccct gcaaatacca *F 721 acagatttgc cttgctggta gataccgctg aggacgtgcc gctgggatcc gttgatatcg *F 781 aaccgaagaa aacaaagcct ccgccaatat acatccgcga gaagagcaca agccgtcttg *F 841 taaatacttt gattggcctt attgggaaag atagctttca tataattccc ctcgtaagag *F 901 gtactatcaa cgaaatcaaa cttcagacga aaacggagga cgactacaga aaagtcacaa *F 961 actattttac cgcacaaaaa ataggcttct acacctacca gcttaaaagc agcaagggcc *F 1021 tgcaagtagt cctgaagggc attgagtctg atgttacgcc cgaagagata actgaggcgc *F 1081 taaaggaaaa gggattttac gccaaaaacg tgttcaatat caaaaacaga aacaggcagc *F 1141 cccaaccact cttcaagatt gagcttgaac cagaaaacaa gcctcctaga aaaaacgagg *F 1201 ttcacccaat ttacaaactc cagctccttt tgcaccgtag gatcacggta gaagagccgc *F 1261 acaaacgcaa cgctcctgta caatgtacaa actgccaaga gtatggccac acgaggtcat *F 1321 attgtacact tcgcccggtg tgcgtagtct gtggagatct ccacgactcc aaacagtgtc *F 1381 aaattaacaa agaaaatgca tgcgagaaaa aatgtaataa ctgcgggggc aatcacacag *F 1441 caaactacag aggctgtcca atctacaaag agctgaaaat ccgtcttcac aaaagaatga *F 1501 acacggcgcg ggcacaccaa ggatcagcta ccctgatacc atcagagaca aatcctgaag *F 1561 taattttctc gaaagcagct agtttcgctc cctggcctac attcaacact aacaagacaa *F 1621 catttgctaa cgttttaaaa tcaggtatga cgcctccaac ccaaaactcc cgaactccac *F 1681 atgaagtgca cacaaaatta gacacacaac aaaactatca cccagctgcg cagcaggaaa *F 1741 caaaaactga agctatgatg caagccttac aacagagcat gatggaattt atgacattta *F 1801 tgaagaccac cattcaagac atgatgcgta atcaaaacct tttgatacaa atgcttgtag *F 1861 cccaacaatc aaataaataa tggctacctt acgcatagct acgtggaacg ccaatggcgt *F 1921 ctcacagcgc aaacttgagc tagctcaatt cctacatgag aagcatatcg acgtaatgct *F 1981 tctttcggaa actcatctca caagcaaata caattttcaa ataagagact accatttcta *F 2041 cggtacaaat catcccgacg gaaaagcaca cggtggcacc gccatactca taaggaaccg *F 2101 tatgaagcac cacttttaca aagaatttgc ggaaaatcat cttcaggcca catctatcaa *F 2161 cattcagctg gatgacaaca ctctccttac actagcggcc gtatactgcc ccccccgttt *F 2221 cacagtatta gaagctcaat tcctggattt cttccaagca ctagggccac acttcattgc *F 2281 agcaggcgac tacaacgcta aacatactca ctggggatcg cgacttgtga acccaaaagg *F 2341 aaaacagctt tataagacga taataaaagc cactaataaa cttgaccatg tttcccccgg *F 2401 gagtcctaca tactggccat cagacctcaa taagctgcca gacctgatcg acttcgcagt *F 2461 tacgaaaaat atttcccgca gtttggttaa agctgaatgt ctgccggatc tctcatctga *F 2521 tcactcgcct gtactaattc acctccgccg atacgcagaa aacgtgaaac caccaaccag *F 2581 attgacctct agcaaaacaa actggctcag gtataaaaaa tatataagtt cacatattga *F 2641 gctaagccca aaactcaata ctgaatctga tatagagagc tgcacgtgtg cattgcaatc *F 2701 catccttact gcagcagctc ttactgcaac acccaaaata acaaataata caattaattc *F 2761 aaaaaagacc aacgtacaaa tcgagcaact cgtccacgta aaacgtcgct tacgcagaga *F 2821 atggcaatct tccagatccc caactgcaaa acaaaagcta aaagtagcca cacggaaact *F 2881 ggccaacgct ctgaaacaag aagaggacga cgatcagcgc cgatacatag agcaactcac *F 2941 accaacaggc acaaaacaaa agtcactgtg gcgagcccac tcaactcttc gcccaccgac *F 3001 tgaaaccgtt ttgccgataa ggaattcatc aggtggctgg gcccgtagtg atgaagacag *F 3061 agccaacaca tttgccgctc acctacaaaa tgtgttcacg ccaaaccagg ctactagcac *F 3121 attcgcgcta ccgtcctatc ccgtaaaccg ccatcagcaa cacaccccaa ttgtgtttcg *F 3181 tcctaaagaa ataactaaaa taatcaaaga caatctcagc ccgaaaaaat cccccggcta *F 3241 cgaccttata acaccggaaa tgatcatcca gctgccacat tctgcagttc gctacataac *F 3301 caagctcttt aatgccatca ccaaacttgg ttactttcca caacgatgga agatgatgaa *F 3361 gatcataatg attccaaagc ctggtaagaa ccacacagtc gcttcatctt acagaccaat *F 3421 aagtctactc tcatgcattt cgaaactatt cgaaaaatgc ctgctgatcc gacttaatca *F 3481 acatctgata taccacaata taatcccagc ccaccaattt ggatttcgcg aaagccacgg *F 3541 aaccattgaa caggtgaatc gtattacaac ggaaataaga actgcatttg aatatcgcga *F 3601 atactgtaca gcagtatttt tagacgtatc ccaagcattc gacaaagtct ggctcgacgg *F 3661 cctaatgttt aaaattaaaa catccctacc cgaaagcaca cacaaacttc taaagtctta *F 3721 cctctatgac agaaagtttg cagtgcggtg caacactgcc acttccactg ttcatacaat *F 3781 tgaggctgga gtcccccaag gcagcgttct tgggccaacc ttatacctca tctatacagc *F 3841 cgacatccct acaaatagtc gcttaacggt atccacattt gccgacgata cagctatcct *F 3901 tagccgttca aggtccccta tccaagctac agcacagttg gcactgtacc tcatcgacat *F 3961 tgagaagtgg ctctctgact ggcgaataaa agtaaacgag caaaaatgca agcacgtgac *F 4021 gtttacgcta aacagacaag actgtcctcc gctcttgttg aacagcatac cactcccgaa *F 4081 agcagacgag gtaacgtacc taggagtaca cctagacaga agactcacat ggcgcaggca *F 4141 cattgaagcc aaaaaaaccc aacttaaact caaagccaac aacttacact ggctcatcaa *F 4201 ctctggttct ccgctcagcc tagatcacaa ggtcttgctc tacaattcta tattgaaacc *F 4261 aatctggacc tatggctcac agttatgggg caatgccagc aacagcaata ttgacatcat *F 4321 tcagcgagca caatcaaaga ttctgagaac catcactggg gcaccgtggt acgttcggag *F 4381 tgaaaacatc caaagagact taaatatccc atcagttacc aacgcaatca cggaacttaa *F 4441 ggaaaaatac catagcaagc ttcacacgca ccccaaccac ctagcgcgag gtctaatcca *F 4501 gctcagcagc cgttcccgtc tccggcgaaa ggacctacca acccagcgaa taaattatta *F 4561 gggccgttta aacatagaac agttggaaaa ataatacaac tgttcaaaaa atacttgtta *F 4621 tagttaagat ttttaaactt attgttagtt cttatacaag aagattcaat aaataaaagc *F 4681 aaagtaaaat aaaaaaaaaa aaaaaaaa *F // # *U FBrf0128180 *a O'Hare *b K. *t 2000.3.8 *T personal communication to FlyBase *u *F >From k.ohare@ic.ac.uk Wed Mar 08 11:58:27 2000 *F To: m.ashburner@gen.cam.ac.uk *F Subject: X element *F Michael, *F I finally got around to preparing and submitting to Genbank the 'novel' *F LINE sequence we had been working on, on and off (more off than on). *F Although I sent it off to Genbank a couple of weeks ago they haven't done *F more than acknowledge my email and give an accession number. I guess they *F are rather busy ? *F Anyway, for what it's worth the accession number will be AF237761. And I'm *F attaching what I sent them as a text file. I have done some recent searches *F and there are clearly copies being sequenced but I only found one complete *F element - the other matches may be partial because they are genuinely *F incomplete elements or because the sequence was 'unfinished'. *F ... *F Kevin *F LOCUS ROXELEMENT 4740 bp DNA INV 22-FEB-2000 *F DEFINITION X element. *F ACCESSION DROXELEMENT *F KEYWORDS . *F SOURCE fruit fly. *F ORGANISM Drosophila melanogaster *F Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; *F Drosophilidae; Drosophila. *F REFERENCE 1 (bases 1 to 4740) *F AUTHORS O'Hare,K., Tudor,M., Davis,A.J., Feldman,M. and Grammatikaki,M. *F TITLE A novel LINE from Drosophila melanogaster, the X element *F JOURNAL Unpublished *F REFERENCE 2 (bases 1 to 4740) *F AUTHORS O'Hare,K., Tudor,M., Davis,A.J., Feldman,M. and Grammatikaki,M. *F TITLE Direct Submission *F JOURNAL Submitted (22-FEB-2000) Biochemistry Department, Imperial College *F of Science, Technology & Medicine, London SW7 2AZ, UK *F FEATURES Location/Qualifiers *F source 1..4740 *F /organism='Drosophila melanogaster' *F CDS 322..1827 *F /note='similar to retrovirus gag proteins with *F cysteine-rich motifs, possible nucleic acid binding *F protein' *F /codon_start=1 *F /product='translation of ORF1' *F /translation='MNTLNETAAADESLDTAFLSSPQCAAPQRFQKIKRKSRASPETE *F RKKPKSTIGKQGENPSATEPRYGGNSNRFGLLAHLTADKQVGNEIGDLYDQPSTSHQA *F AIAAAKRDAASAGTTSSAKRAQSKPPPIVMEGVDDVYLMMQSIENIVDLEKIEARASM *F SGVLRLYAADANTFRTIVNWLEIEEYEFHCYQLKEDRPYRVCVKGLHHSTLHHQIKDE *F LEKIGHKVLDIHTPLRRNEPGTSKASPVNMFFLNIAAAANNKEILAVKALCHMRVVIE *F PLRKRNAIVQCHRCQQIGHTAKYCRKAHICVKCAGEHPAKDCTRPRIELCTCYNCGGQ *F HPANYKGCSKLQAFLQRSRPRSGVAGRTEVSDRPTPRGLAGGKEIPSSRGGISYADVA *F RGSIHHKQPMSLTHQQQKQKQQPYDGSPSRQRSRSRTRASRGTLQRSTDASSSIEAIL *F QTLNENINSLRSIQEKQMELMMMMMKQQQQQSHQQGQIINLLTALQARQAP' *F CDS 1827..4553 *F /note='probable reverse transcriptase' *F /codon_start=1 *F /product='translation of ORF2' *F /translation='MMPLRILVWNADGVSTKLPEVECFVRRHEIDVLLLSETHCKGAE *F TPKLFGFVAYTANDPSGGNAKGGAAILIKNSLAHFPLTPIATAKVQLAPAVIETALGP *F ISFGAVYCPPRFAWTTDEFKDILEEFQTKFIVAGDWNASHWLWGAGRSNQRGIALANL *F VLNSEVDSLATGGPTRYPYGCRGSPGYIDFALTKGVLGIHANISAVVELSSDHLPLVI *F TLDAGAISYPKMERLITRRTNLEVFQSQLESTLPLNTAINSGQDVDDAIELLTNNIKS *F AARLATRSISRQPAADRIPIPREILLLIAEKRRLRTRWMRSRHPSDKTEWNRALSRLR *F CALVLHKAAWFDERLANTGVESEATHSLWKATRAIKRRCTRKAPLVDSNGTWCRTDLG *F QAEVFAAHLAERFQPFKLASLQQVEETQDQLNQALQMDMPITPFEPCEVAEVIVRQSN *F NKAPGHDVICNATLKALPRQAILYITLVFNAIVRLQYFPYQWKLGIISMIHKPGKPER *F EPASYRPISLLPSISKVFERLIAVRIVSIMEAQGITPEHQFGFRAGHCTVEQLHRVVE *F QILTAYDSKEYCNSLFLDIREAFDRVWHIGLQLKIKQTLPAPYFGLLKSYLEGRRFAV *F RFHSAISTEHNVAAGVPQGSVLGPLLYCLYSHDMPQPDVSLYGKSMLATFADDVCVTY *F RSRCEHDAADGIQDFAYRFSEWARRWNIGINSSKSNNVCFTLKRRTPPPVYIEEVPVP *F QPNAAKYLGVLLDRRLTFSKHVTDIRTRLRAKVAKHYWLLSSRSKLSLSNKLTIYKQI *F LAPNWKYGCQIWGLACDSHIKRIQAIQNKVARLITGCEWFVRNTTLHRDLKLATVFDE *F INKHSSRYHDRLERHRNRLASALNRSRPPRRLNRRQPRDLITRSPLTRVRRS' *F BASE COUNT 1336 a 1215 c 1183 g 1006 t *F ORIGIN *F 1 aatgttaaat aaaggttcgt gtctaacaat acgcacctga caaagtggat taagtgaaat *F 61 tagttttcgc ggtaataaac ttatggacaa gaccagaata ctggcacaca tagcaaatag *F 121 tgacccccca agtcactaac agtgaaataa tagtgaaacg aaaacatttt cattcaaaaa *F 181 tacaaagtta agtttctcga actggggctc cgctgcccag ctgccacgcg atcgcacaaa *F 241 cagctgtttg cgagcttaaa gctttctatc ccagggttca agttttggct agaaccctgg *F 301 tgatttggtg cacacttcaa tatgaacact ttaaatgaaa ccgctgcggc tgatgaatcg *F 361 ttggatactg cgtttctctc gagcccccaa tgtgctgccc cgcagcgctt tcaaaaaata *F 421 aagcgaaagt ctcgtgcttc tccggagact gaaaggaaaa aacccaaatc aaccatcggc *F 481 aaacaagggg aaaacccttc ggctacagaa cctagatatg gcggcaattc aaaccgattt *F 541 ggtttacttg cgcatctcac agctgacaaa caagtaggca atgaaattgg cgatctgtat *F 601 gaccagccca gtaccagtca tcaagctgca attgctgccg ctaagcggga tgcagcctcc *F 661 gctggtacca ctagctcagc caaaagagcg cagtccaaac cacctcctat agtaatggag *F 721 ggagtggacg acgtatacct gatgatgcag agcatcgaaa atatagtgga cctagaaaag *F 781 attgaggcta gggcgtcaat gagcggtgtc ctaaggcttt acgcggctga cgctaataca *F 841 tttcgcacca tagtgaactg gctcgagatc gaagagtatg agttccactg ctaccagctt *F 901 aaagaggaca ggccttacag ggtatgcgtg aaaggcctgc accacagtac gctacatcac *F 961 caaatcaagg atgagctgga aaagatcggg cacaaggttc tcgatattca cacaccgctt *F 1021 aggcgaaacg aaccgggtac ctcaaaagcg tcgccagtca atatgttctt cctaaatatt *F 1081 gctgctgcgg caaacaataa ggagatcctg gcggtaaagg cactatgcca tatgagagta *F 1141 gttattgagc ctctccgcaa gcgtaacgct attgtccagt gccatcgttg tcagcagatt *F 1201 ggccacacag ccaaatactg ccgtaaggcc cacatttgtg tgaaatgtgc cggcgaacac *F 1261 ccagccaagg actgtaccag gccacgcatc gagctgtgca cttgctacaa ctgtggcggc *F 1321 cagcatcctg caaactataa aggttgcagc aagctacaag cgttcctgca gcgatccaga *F 1381 cccagaagtg gagtggctgg aagaacagaa gtaagcgatc gaccaactcc acggggctta *F 1441 gctggaggta aggagatccc ctcttctcga ggcggaatat cttatgcaga tgtggctaga *F 1501 gggtccattc accacaagca accaatgagc ctgacgcacc agcaacagaa gcaaaagcaa *F 1561 cagccctatg atggaagccc cagtcgtcaa aggagccgca gccggacaag ggcgtctagg *F 1621 ggtacactcc agcgctcgac ggatgctagc agcagcattg aagccatcct gcagacgctt *F 1681 aatgagaaca ttaattcttt gcgctcgatt caagagaagc aaatggaatt aatgatgatg *F 1741 atgatgaagc aacagcaaca acagtcacat cagcaggggc agattatcaa tctgctcact *F 1801 gctctccaag cgcgtcaagc gccataatga tgccgctgcg catcctagtg tggaacgccg *F 1861 acggcgtatc cacgaagttg cctgaagtag agtgcttcgt gcgacgtcac gaaatcgatg *F 1921 tattactgct cagcgagaca cactgcaagg gggcagagac gcctaagcta ttcggatttg *F 1981 tagcctacac tgccaatgat ccgagtggtg gcaacgccaa aggcggagca gctatcttaa *F 2041 tcaaaaatag ccttgcccac tttccgctaa caccaatagc cactgccaag gtgcaacttg *F 2101 cgccggcggt tattgaaacg gcacttggtc ctataagctt tggagcggtc tactgcccac *F 2161 cgagatttgc atggactacg gacgagttta aggacatttt ggaagagttc cagacgaagt *F 2221 tcattgttgc aggcgattgg aacgcgtccc actggctctg gggtgcggga aggagcaacc *F 2281 aaagaggcat tgcattagcg aatctcgtcc taaattcgga ggtggactcg ctagcaacag *F 2341 gaggaccaac aagatacccg tacggctgta gaggctcacc agggtacatc gattttgcac *F 2401 tgacaaaggg tgtgctgggc atccacgcta acataagtgc ggttgttgag cttagctccg *F 2461 accacctgcc tctggtaatt acgctggatg cgggggcaat atcctaccct aagatggagc *F 2521 ggcttatcac taggcgtact aacctggagg tattccaatc gcaactggag tccacactgc *F 2581 ccctcaacac tgccataaac tctggacagg acgttgatga tgctatcgaa ctgctcacca *F 2641 acaatatcaa gtcagcagct agattggcaa ctcgcagcat atctcggcag cccgcggcag *F 2701 atcgaatccc aatacccagg gagatcctgc tgcttatagc tgagaagagg cgcttacgca *F 2761 ctaggtggat gaggtctcgg cacccgtcgg acaaaacgga atggaaccga gctctgagta *F 2821 ggctccgatg cgcgttggtg ctgcacaaag ccgcatggtt cgacgaaagg cttgccaata *F 2881 ccggagtcga aagcgaagcg acgcattcgc tgtggaaggc cacgcgcgca atcaaaaggc *F 2941 gttgcacgag gaaggcgcct ctagtcgata gcaacgggac atggtgtcgg accgacttgg *F 3001 gacaagcgga ggtattcgct gcgcacctcg ccgagcgatt tcaaccattc aagcttgcca *F 3061 gcctgcaaca ggttgaagaa actcaggacc agctgaacca agcgcttcaa atggatatgc *F 3121 caatcacgcc gtttgaaccc tgcgaggtag ccgaagtcat tgtgcgccag agtaacaaca *F 3181 aagcacctgg acatgacgtc atctgcaacg ccacattgaa ggccctgccc agacaagcga *F 3241 tcctctacat aacgttggtt ttcaacgcta ttgtgaggtt gcaatacttc ccttatcagt *F 3301 ggaagctcgg gataatctcc atgatccaca aacctggcaa gccggaaagg gagcccgcct *F 3361 cctaccggcc gatcagtctc ctcccttcaa tttcgaaggt gtttgagaga ctgattgctg *F 3421 tccggattgt aagcattatg gaagcccagg ggattacccc tgagcaccag ttcggtttcc *F 3481 gtgctggcca ctgtactgtc gagcagctcc atcgagtcgt cgagcaaatt ctgactgcct *F 3541 acgacagtaa ggaatattgt aacagcctct tcttggacat tcgagaagcg tttgatcgag *F 3601 tgtggcacat tggactccaa ctgaaaatca agcagacgct gcctgcccca tattttgggt *F 3661 tgctgaaatc gtacctggaa ggaaggaggt tcgctgtgcg ctttcattca gcaatttcca *F 3721 ccgagcacaa cgtggcagct ggtgttccac aaggtagtgt cctcggcccc ctgctctact *F 3781 gcctgtatag ccacgacatg ccgcagccag atgtaagcct ttacgggaaa tctatgttgg *F 3841 ccacatttgc cgatgacgtg tgcgtcacct acaggtcccg atgcgagcac gacgcagccg *F 3901 atggtatcca ggactttgca taccggttct cggaatgggc aagacgatgg aatattggca *F 3961 tcaatagcag taaatccaac aacgtctgct tcactttaaa gcggagaacg ccaccgcccg *F 4021 tctacatcga ggaagtcccc gtaccacagc cgaacgcagc aaagtacctt ggagtgcttc *F 4081 tggatcgcag actcacattt tccaagcatg tgaccgacat cagaacgcgc ctacgtgcta *F 4141 aggtggcgaa gcactactgg ctactttctt cgcgcagtaa attgtcgcta tccaacaagc *F 4201 tgacaattta caaacagatc ctagcaccaa actggaagta tgggtgccaa atctggggct *F 4261 tagcctgcga cagccacatc aaaaggatcc aggctattca aaataaggta gcaagactca *F 4321 tcaccggctg cgagtggttt gttcgaaaca ccaccctgca cagagacctg aaactcgcaa *F 4381 cggtatttga cgaaataaac aagcactcga gcagatacca tgacaggctg gagcgccaca *F 4441 gaaatcggct ggccagcgct ttaaacagat ctcgcccacc aaggaggctc aatagaaggc *F 4501 aaccgaggga tctcattacc cgatctcctt tgacaagggt ccgcagaagc tgacgcttat *F 4561 cttaaatcct atttgttata tgtgattgtt atgtaattgt agttaaatta ctgtaaattt *F 4621 gaaaaagcta actatagtta gccggcgagc ccaaatgggc tgaattaata gataagaagg *F 4681 acacaaaggg gcttcaagac ttccccgtat gccttaataa ataaattaaa taaaaaaaaa *F // # *U FBrf0128181 *a Roote *b J. *t 2000.5.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 09 16:01:49 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Roote pc for l(2)5CC11B2 *F From: Kevin Cook, Bloomington Stock Center *F Subject: l(2)5CC11B2 allele *F John Roote, Cambridge University, isolated an EMS-induced allele of *F l(2)5CC1 called l(2)5CC11B2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128182 *a Kaufman *b T. *t 2000.5.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 09 16:24:55 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-rpr.C} insertions *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{UAS-rpr.C} insertions *F The following information came from Thom Kaufman, Indiana University (5/00). *F P{w+mC=UAS-rpr.C}14 is a homozygous viable and fertile second chromosome *F insertion. *F P{w+mC=UAS-rpr.C}27 is a homozygous/hemizygous viable and fertile X *F chromosome insertion. *F The construction and transformation of P{UAS-rpr.C} was described in Aplin *F and Kaufman, 1997, Mech. Dev. 62(1): 51--60 (FBrf0093060). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128183 *a Bejsovec *b A. *t 2000.5.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 09 16:50:05 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-shi.K44A} insertions *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{UAS-shi.K44A} insertions *F The following information came from Amy Bejsovec, Northwestern University *F (5/3/00). *F P{w+mC=UAS-shi.K44A}3-10 is an insertion on a Sb+ TM3 balancer *F chromosome. When expressed using a uniform Gal4 driver, it provides a very *F high level of endocytotic disruption, comparable to the original shits1 *F mutation. It phenocopies the cell-lethal effect of shits1. *F P{w+mC=UAS-shi.K44A}4-1 is a homozygous/hemizygous viable and fertile X *F chromosome insertion. P{w+mC=UAS-shi.K44A}3-7 is a homozygous viable and *F fertile second chromosome insertion associated with a recessive curled wing *F phenotype. Together these insertions provide a moderate level of *F endocytotic disruption when expressed using a uniform Gal4 driver line. *F Separately they each provide a low level of disruption. *F The construction and transformation of P{UAS-shi.K44A} was described in *F Moline, M. M., Southern, C. and Bejsovec, A. (1999). Directionality of *F Wingless protein transport is essential for wild-type epidermal patterning *F in the Drosophila embryo. Development 126, 4375-4384 (FBrf0111433). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128184 *a Doughty *b G. *t 2000.5.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 09 18:52:58 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{PZ}E(nd)195ry+3550 *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{PZ}E(nd)195ry+3550 *F The following information was provided by Glenn Doughty, Yale University *F (5/9/00). *F The P element construct inserted in E(nd)195ry+3550 is P{PZ}. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128185 *a Luschnig *b S. *t 2000.5.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 09 19:25:10 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UbiGFP.nls} insertions *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{UbiGFP.nls} insertions *F The following information accompanied stocks from Stefan Luschnig, Max *F Planck-Institut für Entwicklungsbiologie (4/00). *F Stefan mobilized P{w+mC=UbiGFP.nls} from one of Ilan Deak's lines and *F obtained the following new insertions: *F P{UbiGFP.nls}X1 X chromosome *F P{UbiGFP.nls}2R1 2R *F P{UbiGFP.nls}2R2 2R distal to P{UbiGFP.nls}2R1 *F P{UbiGFP.nls}3L1 3L *F P{UbiGFP.nls}3L2 3L proximal to P{UbiGFP.nls}3L1 *F All are homozygous viable and fertile. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128186 *a Bloomington Drosophila Stock Center *b ?. *t 2000.5.12 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Sat May 13 00:44:25 2000 *F Subject: l(3)0369903699 is not lethal *F To: flybase-updates@morgan.harvard.edu *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Dated: 12 May 2000 *F Information communicated: *F l(3)0369903699 is better described as a recessive visible mutation than a recessive *F lethal. Homozygote viability is sufficient to drive TM3 from a balanced stock. The *F visible phenotype includes wing vein defects, most commonly a spur on the posterior *F crossvein, and incomplete abdominal tergites. Both phenotypes overlap wild-type. *F v(3)03699 and v(3)0369903699 would be more suitable symbols for this gene and allele. *F P{PZ}l(3)0369903699 should also be renamed P{PZ}v(3)0369903699. *F The assumption that the insertion is responsible for the mutant phenotype has not been *F verified. # *U FBrf0128187 *a Haenlin *b M. *c M. *d Muskavitch *t 2000.5.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu May 18 14:37:51 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{lacW}439 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{lacW}439 insertion *F The P insertion currently described as P{lacZ-un3}439 (FBti0003782) is an *F insertion of P{lacW} (from correspondence with Marc Haenlin, CNRS, *F Toulouse, 5/12/00). It is a homozygous viable and fertile third chromosome *F insertion (from information provided with stocks from Marc Muskavitch, *F Indiana University, 3/00). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0128188 *a Caggese *b C. *t 2000.5.11 *T personal communication to FlyBase *u *F From caggese@biologia.uniba.it Thu May 11 13:04:56 2000 *F To: flybase-help@morgan.harvard.edu *F Subject: CoVa gene *F Dear Sir, *F I am working on the isolation of mutations in the Drosophila gene *F encoding for cytochrome c oxidase subunit Va (CoVa). *F In the CoVa FlyBase Report you annotate three insertion line *F mutations (EP3183, l(3)S01104 and l(3)S011046) as alleles of the CoVa *F gene. *F Since blast analysis of the sequences flanking the insertion sites *F not recognizes the sequence of the CoVa gene and l(3)S011046 is an *F insertion in the adjacent gene CG6946, I would greatly appreciate *F receiving informations on the source of the annotations on the CoVa *F alleles. *F Thank you in advance. *F Yours sincerely, *F Corrado Caggese *F \-- *F Corrado Caggese *F Istituto di Genetica *F Universita di Bari *F via Amendola 165/A *F 70126 Bari, Italia *F tel.+39 080 5443338 *F fax +39 080 5443386 *F e-mail caggese@biologia.uniba.it *F From rd120@gen.cam.ac.uk Fri May 12 12:02:07 2000 *F To: caggese@biologia.uniba.it *F Subject: Re: CoVa gene *F Cc: flybase-help@morgan.harvard.edu *F Dear Corrado, *F Thank you for your mail. *F . *F . *F . *F I have run a BLAST search using the sequence provided by Peter as being *F flanking for l(3)S011046 and it identifies a hit for sequence record *F accession number Y09065. We have Y09065 under the record for CoVa. *F Y09065 describes mRNA and coding region sequence for CoVa, determined *F independently from l(3)S011046, by you - I note. I enclose the result *F at the bottom. *F You wrote: *F >l(3)S011046 is an *F >insertion in the adjacent gene CG6946, *F this is a bit of a puzzle to me - we did not previously know this and *F it seems to contradict the previous assignment of S011046 to the CoVa *F transcription unit. *F CG6946 and CoVa are divergently transcribed though the annotation does *F not yet suggest that they overlap (there are 317 bp between the 5' ends). *F What is the basis for your assigning l(3)S011046 to CG6946? Is the *F identification of CoVa as a BLAST hit with the flanking sequence for *F l(3)S011046 invalid? *F Thank you for writing to us about this - I will correct the errors about *F l(3)S011041 and EP(3)3183 now (though unfortunately the updates will *F take some weeks to reach the public view) and look forward to hearing *F from you about S011046/CG6946. *F With best wishes, *F Rachel. *F . *F . *F . *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- *F From caggese@biologia.uniba.it Fri May 12 17:48:43 2000 *F To: rd120@gen.cam.ac.uk *F Subject: CoVa *F Dear Rachel, *F I think that l(3)S011046 is not an allele of CoVa but it is in *F reality an allele of the CG6946 divergently transcribed gene since the *F insertion site of this mutation is at 24th nucleotide of a 5' *F trascribed sequence (LD29934) of the CG6946 gene. *F Best regards, *F Corrado *F \-- *F Corrado Caggese *F Istituto di Genetica *F Universita di Bari *F via Amendola 165/A *F 70126 Bari, Italia *F tel.+39 080 5443338 *F fax +39 080 5443386 *F e-mail caggese@biologia.uniba.it # *U FBrf0128189 *a Ronsseray *b S. *t 2000.5.15 *T personal communication to FlyBase *u *F Archived. # *U FBrf0128190 *a Bloomington Drosophila Stock Center *b ?. *t 2000.5.18 *T personal communication to FlyBase *u *F From: Kathy Matthews *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: chic[k13321] *F Date: 18 May 2000 *F *F Information communicated: *F Although Spradling et al., 1999 (FBrf0111489) describe chic[k13321] as l(2)k13321, the chromosome carrying this allele is viable. When the stock of this line was provided to Bloomington by the Berkeley Drosophila Genome Project it was noted (by Amy Beaton, 25 Feb 1998) that 'chi[k13321] is not a lethal'. The stock is homozygous for this second chromosome, indicating that chic[k13321] *F is viable and fertile. # *U FBrf0128191 *a Warr *b C. *c L. *d Vosshall *t 2000.5.19 *T personal communication to FlyBase *u *F From ag24@gen.cam.ac.uk Fri May 19 15:14:37 2000 *F To: rd120@gen.cam.ac.uk *F Subject: Odorant receptors *F Dear Aubrey, *F .. *F I'm a postdoc in John Carlson's lab, and in collaboration with Leslie *F Vosshall I have renamed all the Drosophila odorant receptor genes we know *F about according to an agreed upon nomenclature. A table of the 60 genes we *F know about is attached, showing previous names and the new names. 54 of the *F genes are annotated, 6 are not and Leslie is contacting Flybase about *F getting these annotated as she is publishing them in a paper. *F So all people we know about working on these genes have agreed to this *F scheme, and would like the Flybase/Gadfly records to use the new names as *F the primary name of the genes. *F .. *F If you have any queries please let me know. *F Regards, *F Coral Warr *F \------------------------- *F \# Carlson name Other names Annotation Location New name *F 1 1A.1 DOR68 CG17885 1A8 Or1a *F 2 2F.1 'DOR62, AN4' CG3206 2E1 Or2a *F 3 7D.1 DOR30 CG10759 7D14 Or7a *F 4 9E.1 DOR95 CG15302 9E.1 Or9a *F 5 10B.1 DOR92 CG17867 10B15 Or10a *F 6 13F.1 DOR38 CG12697 13F16-18 Or13a *F 7 19A.1 DOR37 none yet 19B3-19C Or19a *F 8 22A.1 (c) 'DOR53, AN11' CG12193 22A5 Or22a *F 9 22A.2 (c) 'DOR67, AN12' CG4231 22A5 Or22b *F 10 22C.1 DOR16 CG15377 22C1 Or22c *F 11 23A.1 'DOR64, AN5' CG9880 23A3 Or23a *F 12 24D.1 DOR48 CG11767 24E4 Or24a *F 13 30A.1 DOR56 CG13106 30A3 Or30a *F 14 33B.1 (c) 'DOR73, AN3' CG16960 33B10 Or33a *F 15 33B.2 (c) 'DOR72, AN1' CG16961 33B10 Or33b *F 16 33B.3 (c) 'DOR71, AN2' CG5006 33B10 Or33c *F 17 36E.1 DOR91 CG17868 35D1 Or35a *F 18 41E.2 (c) DOR117 CG17250 42A2 Or42a *F 19 41E.1 (c) DOR118 CG12754 42A2 Or42b *F 20 43B.1 'DOR87, AN14' CG1854 43A1 Or43a *F 21 25A.1/43 'AN7, DOR81' CG17853 43F5 Or43b *F 22 45C.1 DOR58 CG1978 45C5 Or45a *F 23 45F.1 DOR107 CG12931 45F1 Or45b *F 24 46F.1 (c) 'AN9, DOR20' CG17849 46E7-8 Or46a *F 25 46F.2 (c) 'DOR19, AN8' CG17848 46E7-8 Or46b *F 26 47E.1 'DOR24, AN10' CG13225 47F1 Or47a *F 27 47E.2 DOR25 CG13206 47F6 Or47b *F 28 49A.1 DOR10 CG13158 49A5 Or49a *F 29 49D.1 'AN13, DOR105' CG17584 49D1 Or49b *F 30 56E.1 DOR59 CG12501 56E1 Or56a *F 31 59D.1 (c) 'DOR46, AN6' CG9820 59E1 Or59a *F 32 59E.1 (c) DOR119 CG3569 59E1 Or59b *F 33 59E.2 (c) DOR120 CG17226 59E1 Or59c *F 34 63B.1 DOR28 CG9969 63B1 Or63a *F 35 LU27.2 DOR66 none yet 65A7-11 Or65a *F 36 LU27.1 DOR63 none yet 65A7-11 Or65b *F 37 LU27.3 DOR65 none yet 65A7-11 Or65c *F 38 67B.1 DOR84 CG12526 67B2 Or67a *F 39 67B.2 CG14176 67B10 Or67b *F 40 67D.1 DOR77 CG14156 67D2 Or67c *F 41 69F.1 DOR83 none yet 69E8-F1 Or69a *F 42 69F.2 DOR82 CG17902 69E8-F1 Or69b *F 43 71B.1 DOR14 CG17871 71B1 Or71a *F 44 74A.1 DOR26 CG13726 74A6 Or74a *F 45 DOR61 none yet 82A3-4 Or82a *F 46 83A.1 (c) DOR44 CG10612 83A6 Or83a *F 47 83A.2 (c) DOR45 CG10609 83A6 Or83b *F 48 83D.1 DOR116 CG15581 83D4 Or83c *F 49 85A.4 DOR31 CG7454 85A3 Or85a *F 50 85A.1 (c) DOR115 CG11735 85A9 Or85b *F 51 85A.3 (c) DOR78 CG17911 85A9 Or85c *F 52 85A.2 '85.2, DOR114' CG11742 85A11 Or85d *F 53 DOR104 85B.1 CG9700 85B2 Or85e *F 54 85D.1 DOR32 CG16755 85D15 Or85f *F 55 88A.1 DOR99 CG14360 88B1 Or88a *F 56 92E.1 DOR111 CG17916 92E8 Or92a *F 57 94D.1 (c) DOR108 CG17241 94D9 Or94a *F 58 94D.2 (c) DOR109 CG6679 94D9 Or94b *F 59 98B.1 DOR110 CG5540 98B5 Or98a *F 60 98C.1 DOR121 CG1867 98D4 Or98b # *U FBrf0128192 *a Levis *b R. *t 2000.6.23 *T personal communication to FlyBase *u *F Personal communication from Robert Levis, 23 June 2000 *F *F Subject: P{wA[R]} and P{wA} insertions *F *F The following insertions have multiple designations in FlyBase that need *F to be merged. They were originally described in Levis et al., 1985, Science *F 229:558--561, and are indicated below with the cytology-based designation *F derived from that reference. *F *F P{wA[R]}49D aka P{wA[R]}4-3+4-49b. This was an induced transposition *F from the 40A insertion P{wA[R]}4-3 to 49D that retained the 40A *F insertion. There is a stock with both insertions in the Bloomington *F stock center (BL-P912). This stock is noted in the P{wA[R]}4-3+4-49b *F record but not the P{wA[R]}49D record. The P{wA[R]}4-3+4-49b record *F lists only the 49D insertion site. *F *F P{wA[R]}45D aka P{wAR}4-3+4-45a. Again, this was an induced transposition *F from the 40A insertion P{wA[R]}4-3 to 45D. There is a stock with both *F insertions in the Bloomington stock center (BL-P924). This stock is *F noted in the P{wA[R]}4-3+4-45a record but not the P{wA[R]}45D *F record. The P{wA[R]}4-3+4-45a record lists only the 40A insertion *F site. *F *F P{wA[R]}91AB - I could not find P{wA[R]}91AB in the list of P insertions *F in the 91AB region. However, there are two insertions in the database that *F I am certain are the same as this one. They are designated P{wA[R]}fru[0-1] *F and P{wA[R]}fru[2]. Our original isolation name for this insertion was O-1 *F (letter O, number 1) and that would have been the name in the Rubin lab *F stocks. The reference for fru[O-1] is Moses, Ellis and Rubin (1989) *F (FBrf0050650). I haven't looked for this paper, but I infer from the *F allele name that after I left the Rubin lab, Kevin Moses et al. apparently *F determined that this was an insertion near fru. Either he or someone else *F named the allele fru[O-1]. The primary reference listed for fru[2] in Flybase *F is: Gailey and Hall, 1989 Genetics 121: 773--785 (FBrf0049929). The Materials *F & Methods of that paper states: "P(w[+])ARO-1 is a transposon...derived from *F moblilization of the transposon P[(w,ry)A[R]]4-3 and reinsertion at the *F cytogenetic region 91B1,2 (Levis, Hazelrigg and Rubin 1985; K. Moses, personal *F communication). This transposon is maintained in the stock w[1118], *F TM3/ARO-1, which was provided to us by K. Moses and G.M. Rubin." In this paper *F Gailey and Hall refer to the insertion as ARO-1, but never designate it as an *F allele of fru. It was evidently renamed fru[2]. The gene report for fru *F lists [O-1] and [2] as separate alleles even though it seems clear to me that *F they are the same. *F *F P{wA[R]}94E - I could not find P{wA[R]}94E in the list of 94E *F insertions. However, I did find P{wA[R]}4-94, which was the designation *F that I originally gave a stock with this inserion when I sent it to *F the Bloomington stock center. In addition, Flybase lists an *F insertion P{wA[R]}unk[OR20] that is almost certainly the same thing. *F Our original name for this insertion was "O20(1C)". The references *F for P{wA[R]}unk[OR20] are *F Mohler et al. 1991, 1992, 1995. I don't have copies of these papers *F or journals handy. But Mohler and Vasilakis 1992 (DIS 71: 250), in a *F paper about a daeh mutation on a TM3 chromosome, state "We obtained *F this variant TM3 chromosome,..., from B. Levis in a strain carrying *F a P(w,ry)A insertion in the unkempt locus in 94E (Levis et al. *F 1985)..." *F *F P{wA[R]}92B - In Levis et al. 1985 we reported two independent hops from *F AR4-3 to 92B. Flybase lists one of these insertions as *F P{wA[R]}4-921, which is the name I gave for one of these stocks when *F I sent it to the Bloomington stock center. I suggest designating the *F second P{wA[R]}4-922. *F *F P{wA}l(2)46C - The 46C insertion derived from transposition of P{wA}4-4 *F (as described by Levis et al. 1985) is designated P{wA[R]}4-46 in FlyBase. *F The [R] here is incorrect, this should be simply P{wA}4-46 (or P{wA}46C, *F if you prefer). The second record for this same insertion is *F P{wA}l(2)46Cb[N21]. Our original name for this insertion was N21 and *F the record for P{wA}l(2)46Cb[N21] lists Levis et al. 1985 as the *F reference for the cytogenetic location. The other reference for *F P{wA}l(2)46Cb[N21] is O'Brien et al. 1994, in which Levis et al. 1985 *F is referenced for the N21 insertion. *F *F P{wA[R]}34E - An unpublished insertion in 34E is designated in *F Flybase as P{wA[R]}4-34. This was a transposition from an *F unpublished 41A insertion originally called B133-0923. My original *F name for the 34E insertion was 11P. There is another 34E insertion *F in Flybase designated P{wA[R]}rk[11P]. The only reference given in *F the record for this insertion is Roote et al. 1997.6.2, which is a *F personal communication that I can't make sense out of (it may not be *F the right reference). The record for the rk[11P] allele lists *F P{wA[R]}B133-0923 as the progenitor and R. Levis as the discoverer. *F *F *F The following insertions I would prefer to change to a consistent *F designation: *F *F The insertion at 10D is currently designated P{wA[R]}038. I prefer *F P{wA[R]}4-10 or P{wA[R]}10D. *F *F You might also want to edit the Flybase records for the 41A insertion *F B133-0923 and two insertions derived by transpositions from it. The 41A *F insertion is designated P{wA[R]}B133-0923. This was the original *F isolation number for this stock. When I sent the stock to the *F Bloomington stock center, I called it P[(w,r)A[R]]4-0923 (not listed in *F Flybase). I suggest designating this insertion P{wA[R]}4-41 or P{wA[R]}41A. *F This insertion confers a yellow-red speckled eye color because of PEV on *F the white transgene. Sass & Henikoff 1998 (FBrf0102004) studied the *F modification of the eye color of this transgene insertion by Suvars & Evars. *F They refer to it simply as B133. You might want to add this reference and *F B133 and P{wA[R]}4-0923 as synonyms to the record for this insertion. The *F two insertions derived from B133-0923 are designated in Flybase as *F P{wA[R]}4-0923+4-49a and P{wA[R]}4-0923+4-52a. The records for each *F of these insertions list the only cytogenetic location as 41A. *F However, there are stocks for both in Bloomington and if you look up *F the stock records, you find that P{wA[R]}4-0923+4-49a has both a 41A *F insertion and a 49C insertion and that P{wA[R]}4-0923+4-52a has both *F a 41A insertion and a 52C insertion. As with some of the 40A-derived *F transpositions, these two transpositions from 41A retained the *F original insertion. There is no indication in these Flybase records *F that the 41A insertion in P{wA[R]}4-0923+4-49a and P{wA[R]}4-0923+4-52a *F is the same as the insertion designated P{wA[R]}B133-0923. You might want *F to consider renaming P{wA[R]}4-0923+4-49a, P{wA[R]}4-0923+4-52a, *F P{wA[R]}4-3+4-45a and P{wA[R]}4-3+4-49b. I suggest designating these *F P{wA[R]}49C, P{wA[R]}52C, P{wA[R]}45D and P{wA[R]}49D, respectively. *F *F _________________________ *F Robert W. Levis *F Syracuse University *F Department of Biology *F Biological Research Labs *F 130 College Place *F Syracuse, NY 13244-1220 *F *F PH - 1-(315)443-9948 *F FAX - 1-(315)443-2012 *F EMAIL rwlevis@syr.edu *F _________________________ # *U FBrf0128208 *a Erickson *b J. *t 2000.4.11 *T personal communication to FlyBase *u *F From jwe7@columbia.edu Wed Apr 12 01:41:11 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: l(1)G0169 identity *F We have performed complementation tests with the l(1)G0169 gene (Bloomington *F stock P-1874) and discovered that it is an allele of l(1)10Bb. The P-1874 *F stock fails to complement l(1)10Bb1 but the double mutant can be rescued by *F l(1)10Bb+ P element transgenes. The P insertion was originally reported to *F map at 10B1-2 whereas l(1)10Bb mapped to 10B4-5 (where it belongs); however, *F the sequencing project moved l(1)10Bb to 10B11. The reported yolk nuclear *F (vitellophage) staining of this enhancer trap is likely due to its proximity *F to the vitellophage-specific enhancer of the adjacent sisA gene. We do not *F have any molecular data on the insertion site. *F Jim Erickson *F James W. Erickson *F Assistant Professor *F Department of Biological Sciences *F Columbia University *F 600 Fairchild *F 1212 Amsterdam Ave Mail Code 2402 *F New York, NY 10027 *F Phone: 212-854-4625 *F FAX: 212-865-8246 # *U FBrf0128209 *a Mount *b S. *t 2000.5 *T personal communication to FlyBase *u FlyBase error report on April 5, April 28 and May 12 2000. *F From smount@wam.umd.edu Wed Apr 05 19:37:01 2000 *F Date: Wed, 5 Apr 2000 14:41:28 -0400 (EDT) *F From: Stephen M Mount *F To: Michael Ashburner *F Subject: RE: drosophila snRNAs (fwd) *F Message-ID: *F *F Michael, *F *F This is what I sent to Celera for the annotation. The Excel file is being *F sent separately. *F *F Steve *F *F ---------- Forwarded message ---------- *F Date: Mon, 28 Feb 2000 21:16:48 -0500 (EST) *F From: Stephen M Mount *F To: 'Skupski, Marian P.' *F Subject: RE: drosophila snRNAs *F *F Marian, *F *F This email contains, in the body of the text, NOT as attachments, *F *F 1) established Drosophila snRNA sequences for U1, U2, U4, U5 and U6 *F *F 2) hypothetical Drosophila snRNA sequences for U4atac, U6atac and U12 *F *F 3) updated versions of the summary files I sent yesterday *F *F The latter includes the three additional U2 genes that were missing *F yesterday. *F *F You may want to correlate these with existing (published) snRNA genes *F for U1, U2, U4 and U6. *F *F Note also that I found U5 genes that have variant 3' termini and may not *F be real genes at all. *F *F I hope this does it for you. If not, feel free to contact me again. *F *F Steve Mount *F *F \################################################## *F 1) established Drosophila snRNA sequences for U1, U2, U4, U5 and U6 *F *F What follows are established Drosophila snRNA sequences; most are based on *F RNA sequencing. U3 is not a spliceosomal RNA, and I did not investigate *F it. *F *F > U1 RNA ACCESSION X04257 *F atacttacctggcgtagaggttaaccgtgatcacgaaggcggttcctccggagtgaggcttggccattgcacctcggctg *F agttgacctctgcgattattcctaatgtgaataactcgtgcgtgtaatttttggtagccgggaatggcgttcgcgccgtc *F ccga *F *F >U2 RNA *F atcgcttctcggccttatggctaagatcaaagtgtagtatctgttcttatcagcttaacatctgatagttcctccattgg *F aggacaacaaatgttaaactgatttttggaatcagacggagtgctaggggcttgctccacctctgtcgcgggttggcccg *F gtattgcagtaccgccgggatttcggcccaac *F *F >U4 RNA ACCESSION K03095 *F agcttagcgcagtggcaataccgtaaccaatgaagcctccctgaggtgcggttattgctagttgaaaactttaaccaacc *F cacgccatgggacgtgaaataccgtccactacggcaatttttggaagcccttacgagggctaa *F *F > U5 RNA. ACCESSION K03096 *F atactctggtttctcttcaatgtcgaataaatctttcgccttttactaaagatttccgtggagaggaacactctaagagt *F ctaaaactaattttttagtcagtcttgtcgcaagactggggcca *F *F > U6 RNA. ACCESSION X06669 *F gttcttgcttcggcagaacatatactaaaattggaacgatacagagaagattagcatggccccagcgcaaggatgacacg *F caaaatcgtgaagcgttccacattttt *F *F \################################################## *F 2) hypothetical Drosophila snRNA sequences for U4atac, U6atac and U12 *F *F What follows are HYPOTHETICAL Drosophila snRNA sequences; based on the *F genomic sequence. I found these with blast searches using modified *F parameters. *F *F > hypothetical fly U4atac; What's depicted here is the region of *F similariy; the actual RNA may extend beyond these nucleotides. *F accttccttgtcttggggagcagaaatgttcaatgaacgtctagtgaggacattgctgctgacaccaatgatgacacccc *F cgctcgccgatcgttcgcgattggagttcggaatttttgga *F *F > hypothetical fly U6atac; *F gtggtccaaacgtgttgtttggaaggagagcaagttagcactcccctagacaaggatggaacacataaacggtcggctag *F gcacagacaaaagccgtccacaaattttt *F *F > hypothetical fly U12 RNA; matches vertebrate U12 snRNA at both *F ends. gtgcctcaaactaatgagtaaggaaaaccaatcagccttgctaatcgcttggcagtattggcttctaggcaggg *F gggcgt *F gtcccgcgccccttgaagctcaaatttttgcaagggcacaggtcgtcccctcctcctccgcgtgggtggcgttcggccga *F gcgaaccggcgcctactttgcgtccggctagcgaggatctctgggtgccatcccacggctgggtgttgcgatctgccc *F *F *F Support for U4atac: *F *F gb|AC013956.1|AC013956 Drosophila melanogaster, \*** SEQUENCING IN *F PROGRESS \***, in ordered *F pieces *F Length = 65679 *F *F Score = 52.1 bits (116), Expect = 5e-06 *F Identities = 88/129 (68%), Gaps = 13/129 (10%) *F Strand = Plus / Minus *F *F *F Query: 2 accatccttttcttggggttgcgctactgtccaatgagcgcatagtgagggcagtactgc *F 61 *F ||| ||||| |||||||| || | ||| |||||| || |||||||| || | |||| *F Sbjct: 1325 accttccttgtcttgggga-gcagaaatgttcaatgaacgtctagtgaggacattgctgc *F 1267 *F *F *F Query: 62 taacgcc--tgaacaacacacccgcatcaactagagcttttgc---tttattttggtgca *F 116 *F | || || || | |||| ||||| || | || | || || | | || || | *F Sbjct: 1266 tgacaccaatg-atgacacccccgc-tcgcc--gatcgttcgcgattggagttcgg---a *F 1214 *F *F *F Query: 117 atttttgga 125 *F ||||||||| *F Sbjct: 1213 atttttgga 1205 *F *F *F \################################################## *F 3) updated versions of the summary files I sent yesterday *F *F snRNA promoter hits: *F *F AC018327.1C taattcccaactagttctagttgcgccctcatggaaa U1-82.3 001 *F AC015109.1C caattcccaactggttttagctgctcagccatggaaa U1-95.1 002 *F AC019896.1C caattcccaactgcttctggccgtttgctcatggaag U2 003 *F AC019896.1C gaattcccaactgcttctggccgtttggtcatggaag U2 004 *F AC015154.1C taattcccaactggttctggctacttccctatggaga U1-95.2 005 *F AC015154.1C aaattcccaaacagttctggcagatctctcaaggaga U1-95.3 006 *F AC017493.1C taattcccaactgcttctggccatcagctcatggaaa U1-21.1 007 *F AC019965.1C taattcccaaatggttctggccgtttgcccatcgaga U2 008 *F AC015392.1C taattcccaactgctactggctgcgcttgcatggagt ?? 009 *F AC017832.1C taattcccaaatggttctggcttgctgtgaatggaat U4-1 010 *F AC014745.1C taattcccaactgcttctggcagcgccggcatggtat U4-2 011 *F AC019965.1C tgattcccaacatgttcaagctcgttctaaatgatcg U5 012 *F AC019603.1C taattcccaacgtgttaaagcagtcactgaatagagt U5 (RNA) 017 *F AC019905.1C gaattcccaaaaagttctatcacagaacgaatctagg U5 018 *F AC017727.1C tgattcccaacacgttcaagcaatttcttagtggtac U5 019 *F AC019732.1C aaattcccaactccttctggccaacactgatcctaga U5 var. 020 *F AC017832.1C taattcccaagcggttctattcaatattgagtatgga U5 var. 021 *F AC017832.1C aaattcccaattccttctggccaatactgatcctaga U5 var. 022 *F AC014181.1C tgattcccaaccggttctggttgcatggccatgagtt U12 013 *F AC018038.1C tgattcccaagtacatattctgcaagagtacagtata U6-1 014 *F AC018038.1C taattctcaactgctctttcctgatgttgatcattta U6-2 015 *F AC018038.1C taattctcaacttctttttccagactcagttcgtata U6-3 016 *F AC018217.1C aaattcccaagttctttttccgcatggagtgcttata U6atac 023 *F AC013956.1C taattcccaactagtactggccacttttgcttgaggt U4atac 024 *F AC017832.1C taattcccaactgattttagctgcagtcgcatgaagt U2 025 *F AC017832.1C taattctcaactgattttagctgcagtcgcatgaagt U2 026 *F AC019732.1C taattcccaactggtcttggctgcagtcgcatcaagt U2 027 *F *F Small nuclear RNA gene report. 2-28-00 *F *F I found 5 genes for U1, 6 genes for U2, 2 genes for U4, 7 genes for U5 (3 *F of which are quite divergent at the 3' end), 3 genes for U6, 1 gene for *F U12, 1 gene for U6atac and 1 gene for U4atac. *F *F Sequences for Drosophila U1, U2, U4, U5 and U6 RNAs were known. Finding *F the genes for U4atac, U6atac and U12 absolutely required varying the blast *F search parameters. -r 10 -q -11 -W 7 -G 15 -E 4; -r 7 -q -14 -W 7 *F -G 7 -E 3; and -r 4 -q -5 -W 8 -G 10 -E 2 were among the more *F successfull parameter sets. *F *F Details follow below. Unfortunately, I did these searches through GenBank, *F and so I have GenBank accession numbers rather than Celera numbers. *F *F That these are indeed snRNA genes is clear from their promoter *F sequences. ALL of these genes have a characteristic snRNA promoter *F properly placed upstream of the RNA, so I have a high confidence in *F them. I do not mention U11 because I could not find anything that was *F compelling; the nature of snRNA conservation is such that I could be *F missing something. *F *F U1 -- five genes, all of which were previously described: *F *F gb|AC018327.1|AC018327 Drosophila melanogaster, \*** SEQUENC... 327 *F 8e-89 *F my 001; U1-82.3 *F *F gb|AC017493.1|AC017493 Drosophila melanogaster, \*** SEQUENC... 311 *F 5e-84 *F my 007; U1-21.1 *F *F gb|AC015109.1|AC015109 Drosophila melanogaster, \*** SEQUENC... 311 *F 5e-84 *F my 002; U1-95.1 *F *F gb|AC015154.1|AC015154 Drosophila melanogaster, \*** SEQUENC... 311 *F 5e-84 *F my 005; U1-95.2 *F my 006; U1-95.3 *F *F Additional U1-related sequences at 82E: 82.1 and a pseudogene (82.2) are *F missing. *F Their absence may be a polymorphism (due to recombination); the published *F sequences of the real genes, 82.1 and 82.3 are identical, and they were *F known to be directly oriented on the phage clone. *F Alternatively, the tandem repeat could have caused an assembly error. *F *F *F U2 -- six genes and what it probably a pseudogene. I have yet to work out *F the relationship between these genes and what was previously published. *F *F gb|AC019896.1|AC019896 Drosophila melanogaster, \*** SEQUENC... 381 *F e-105 *F 2 genes (my 003, 004) *F *F gb|AC019965.1|AC019965 Drosophila melanogaster, \*** SEQUENC... 373 *F e-102 *F *F gb|AC017832.1|AC017832 Drosophila melanogaster, \*** SEQUENC... 373 *F e-102 *F 2 genes (my 025, 026). There is also a U4 gene and two U5 genes in *F this contig. *F *F gb|AC019732.1|AC019732 Drosophila melanogaster, \*** SEQUENC... 365 *F e-100 *F *F gb|AC014977.1|AC014977 Drosophila melanogaster, \*** SEQUENC... 52 *F 8e-06 *F A pseudogene ? *F *F *F U4 -- two genes, both previously published. *F *F gb|AC017832.1|AC017832 Drosophila melanogaster, \*** SEQUENC... 297 *F 6e-80 *F my 010; U4-1. There are also two U2 genes and two U5 genes in this *F contig. *F *F gb|AC014745.1|AC014745 Drosophila melanogaster, \*** SEQUENC... 238 *F 5e-62 *F my 011; U4-2 *F *F *F U5 -- 4 genes and 3 variant genes *F *F gb|AC019603.1|AC019603 Drosophila melanogaster, \*** SEQUENC... 143 *F 2e-33 *F This matches the published U5 RNA sequence for Drosophila U5. My *F number 017. *F *F *F gb|AC019965.1|AC019965 Drosophila melanogaster, \*** SEQUENC... 141 *F 7e-33 *F This differs from the published U5 RNA in the 3' end stem-loop. *F My number 017. *F *F gb|AC019905.1|AC019905 Drosophila melanogaster, \*** SEQUENC... 139 *F 3e-32 *F This differs from the published U5 RNA in the 3' end stem-loop. *F My number 018. *F *F gb|AC017727.1|AC017727 Drosophila melanogaster, \*** SEQUENC... 139 *F 3e-32 *F This differs from the published U5 RNA in the 3' end stem-loop. *F My number 019. *F *F gb|AC019732.1|AC019732 Drosophila melanogaster, \*** SEQUENC... 137 *F 1e-31 *F This differs greatly from the published U5 RNA in the 3' end *F stem-loop. *F My number 020. *F *F gb|AC017832.1|AC017832 Drosophila melanogaster, \*** SEQUENC... 139 *F 3e-32 *F These two genes differ greatly from the published U5 RNA in the 3' *F end stem-loop. *F My 021 and 022. There are also two U2 genes and a U4 gene in this *F contig. *F *F *F U6 -- 3 genes on a single contig. These were all previously described. *F *F gb|AC018038.1|AC018038 Drosophila melanogaster, \*** SEQUENC... 212 *F 2e-54 *F *F U11 -- MISSING! There is nothing in the genome that convincingly matches *F U11. However, given the difficulty of finding highly divergent snRNAs, *F this result is inconclusive. I tried hard, varying parameters and checking *F all sites that match the snRNA promoter consensus, but I did not find any *F convincing hits. *F *F U12 -- 1 gene. *F *F gb|AC014181.1|AC014181 *F nucleotides 16743-16506 (238 nt.). *F *F U6atac -- 1 gene. *F *F gb|AC018217.1|AC018217 Drosophila melanogaster, \*** SEQUENC... 74 *F 1e-12 *F *F U4atac -- 1 gene *F *F gb|AC013956.1|AC013956 Drosophila melanogaster, \*** SEQUENC... 52 *F 5e-06 *F *F \################################################## *F *F On Mon, 28 Feb 2000, Skupski, Marian P. wrote: *F *F > Steve *F > *F > The sequences will be good enough. We're spending the next week or so *F > getting the data ready to go to GenBank, and I can blast the sequences *F and *F > get coordinates ready if I have the sequences. I'll try to get the two *F > extra U2 sequences added to the count that you sent to Mark. *F > *F > marian *F *F *F \################################################## *F *F Stephen M. Mount *F Cell Biology and Molecular Genetics *F H. J. Patterson Hall *F University of Maryland *F College Park, MD 20742-5815 *F *F Phone 301-405-6934 *F FAX 301-314-9081 *F permanent email address sm193@umail.umd.edu *F *F \################################################## *F *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F From smount@wam.umd.edu Fri Apr 28 04:08:17 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 04:08:17 +0100 *F X-Authentication-Warning: rac9.wam.umd.edu: smount owned process doing -bs *F Date: Thu, 27 Apr 2000 23:13:05 -0400 (EDT) *F From: Stephen M Mount *F To: Michael Ashburner *F cc: flybase-help@morgan.harvard.edu, Helen Salz *F Subject: snRNA report. *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='-559023410-851401618-956891585=:3153' *F Content-Length: 21169 *F *F Michael, *F *F You suggested that I send you a report of snRNA positions. Here it is! *F *F I have not renamed ANYTHING. New genes have been named according to the *F system used for the old names. Notes relevant to this are at the bottom, *F below my assignments and above the reference RNA sequences. *F *F It is interesting that there are three or four clusters of snRNA genes: *F *F AE003639 at 34A -- three U2 genes and one U5 gene in 35 kb. *F *F AE03664 at 38AB -- two U2, one U4, and two U5 genes in 6 kb.!!! *F *F AE003501 at 14B -- a U2 gene and a U5 gene in 1 kb. *F *F AE003604 and AE003603 at 82E -- two U1 genes and a U4atac gene some *F unknown distance apart in beta heterochromatin. *F *F \################################################## *F *F On Sun, 2 Apr 2000, Michael Ashburner wrote: *F *F > Steve *F > *F > All the tRNAs are are in the xml, since they are displayed on Genescene. *F > Suzi might be able to provide a list with coordinates and (predicted) *F > cytology. *F > *F > We might be able to give you some help in correlating existing snRNA *F > genes with the Celera sequence. Aubrey can, I am sure, translate coordinates into *F > approximate cytology and that might help. *F > *F > I would like to avoid, if at all possible, the naming of snRNA/tRNA genes from *F > the Celera sequence independent of what FB has already. *F > *F > Michael *F *F \################################################## *F *F Gene name Promoter sequence and spacing to RNA Accession *F RNA location *F *F U1-21D taattcccaactgcttctggccatcagctcatggaaa 24 AE003588 *F 18,650 18,813 *F *F U1-82Ea taattcccaactagttctagttgcgccctcatggaaa 24 X53542 *F 2,592 2,755 *F *F U1-82Ec taattcccaactagttctagttgcgccctcatggaaa 24 AE003604 *F 196,233 196,396 *F *F U1-95Ca caattcccaactggttttagctgctcagccatggaaa 24 AE003745 *F 55,799 55,636 *F *F U1-95Cb taattcccaactggttctggctacttccctatggaga 24 AE003745 *F 23,856 24,027 *F *F U1-95Cc aaattcccaaacagttctggcagatctctcaaggaga 24 AE003745 *F 22,328 22,491 *F *F U2-14B taattcccaactggtcttggctgcagtcgcatcaagt 25 AE003501 *F 221,890 222,081 *F *F U2-34ABa caattcccaactgcttctggccgtttgctcatggaga 25 AE003639 *F 91,282 91,091 *F *F U2-34ABb gaattcccaactgcttctggccgtttggtcatggaga 25 AE003639 *F 95,005 95,196 *F *F U2-34ABc taattcccaaatggttctggccgtttgcccatcgaga 25 AE003639 *F 123,728 123,537 *F *F U2-38ABa taattcccaactgattttagctgcagtcgcatgaagt 25 AE003664 *F 83,768 83,577 *F *F U2-38ABb taattctcaactgattttagctgcagtcgcatgaagt 25 AE003664 *F 80,609 80,821 *F *F U4-38AB taattcccaaatggttctggcttgctgtgaatggaat 25 AE003664 *F 78,697 78,838 *F *F U4-39B taattcccaactgcttctggcagcgccggcatggtat 25 AE003669 *F 203,879 203,737 *F *F U4-25F taattctcaaaaggttttagcagactccgcatagaga 24 AE003610 *F 126,856 127,003 *F *F U5-14B aaattcccaactccttctggccaacactgatcctaga 26 AE003501 *F 221,335 221,226 *F *F U5-23D gaattcccaaaaagttctatcacagaacgaatctagg 25 AE003581 *F 87,935 87,805 *F *F U5-34A tgattcccaacatgttcaagctcgttctaaatgatcg 25 AE003639 *F 124,015 124,141 *F *F U5-35D tgattcccaacacgttcaagcaatttcttagtggtac 25 AE003648 *F 179,845 179,720 *F *F U5-38ABa taattcccaagcggttctattcaatattgagtatgga 24 AE003664 *F 80,037 79,911 *F *F U5-38ABb aaattcccaattccttctggccaatactgatcctaga 26 AE003664 *F 84,377 84,503 *F *F U5-63BC taattcccaacgtgttaaagcagtcactgaatagagt 24 AE003477 *F 26,662 27684 *F *F U6-96Aa tgattcccaagtacatattctgcaagagtacagtata 27 AE003748 *F 102,469 102,574 *F *F U6-96Ab taattctcaactgctctttcctgatgttgatcattta 26 AE003748 *F 103,072 103,178 *F *F U6-96Ac taattctcaacttctttttccagactcagttcgtata 26 AE003748 *F 103,595 103,701 *F *F U4atac-82E taattcccaactagtactggccacttttgcttgaggt 21 AE003603 *F 245,404 245,525 *F *F U6atac-29B aaattcccaagttctttttccgcatggagtgcttata 26 AE003621 *F 31,949 32,045 *F *F U12-73B tgattcccaaccggttctggttgcatggccatgagtt 25 AE003526 *F 210,776 210,539 *F *F *F Notes: *F *F U1-82Ea is not in the Celera sequence. That could be due to a strain *F difference or to an error in sequence assembly (the U1-82Ea and U1-82Ec *F genes are located nearby and are nearly identical [Lo & Mount. Nucleic *F Acids Res. 18: 6971-6979]). However, it should be noted that U1-82Ea *F encodes the variant U1b which differs from U1a by a single nucleotide and *F is known to be expressed in Kc cells and Oregon R flies. U1-82Eb is a *F pseudogene and so is not listed. It lies between U1-82Ea and U1-82Ec and *F is also missing from the Celera sequence. U1-95Cc encodes the U1c variant. *F *F I have used the term U4d for U4-25F to avoid confusion. The U4a, U4b and *F U4c sequences published by Guthrie and Patterson (A.R. Genetics 1988) are *F attributed to Kiss, unpublished, and the U4-38AB gene resembles both U4a *F and U4c (sometimes one and sometimes another) at points where they differ. *F *F No U5 gene entirely agrees with the published RNA sequence. Distances to *F the are cap site are therefore approximate. *F *F ------------------------------------ *F *F RNA sequences (for confirmation): *F *F >gi|174317|gb|K00787.1|DROUR1A D. melanogaster U1 small nuclear RNA *F GATACTTACCTGGCGTAGAGGTTAACCGTGATCACGAAGGCGGTTCCTCCGGAGTGAGGCTTGGCCATTG *F CACCTCGGCTGAGTTGACCTCTGCGATTATTCCTAATGTGAATAACTCGTGCGTGTAATTTTTGGTAGCC *F GGGAATGGCGTTCGCGCCGTCCCGA *F *F >U2 snRNA (from various) *F atcgcttctcggccttatggctaagatcaaagtgtagtatctgttcttatcagcttaacatctgatagttcctccattgg *F aggacaacaaatgttaaactcatttttggaatcagacggagtgctaggggcttgctccacctctgtcacgggttggcccg *F gtattgcagtaccgccgggatttcggcccaac *F *F >gi|174319|gb|K03095.1|DROUR4 D. melanogaster U4 small nuclear RNA *F AGCTTAGCGCAGTGGCAATACCGTAACCAATGAAGCCTCCCTGAGGTGCGGTTATTGCTAGTTGAAAACT *F TTAACCAACCCACGCCATGGGACGTGAAATACCGTCCACTACGGCAATTTTTGGAAGCCCTTACGAGGGC *F TAA *F *F >gi|174320|gb|K03096.1|DROUR5 D. melanogaster U5 small nuclear RNA, 3' end *F ATACTCTGGTTTCTCTTCAATGTCGAATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACA *F CTCTAAGAGTCTAAAACTAATTTTTTAGTCAGTCTTGTCGCAAGACTGGGGCCA *F *F >gi|8768|emb|X06669.1|DMU6 Drosophila melanogaster U6 snRNA *F NGTTCTTGCTTCGGCAGAACATATACTAAAATTGGAACGATACAGAGAAGATTAGCATGGCCCCAGCGCA *F AGGATGACACGCAAAATCGTGAAGCGTTCCACATTTTT *F *F > Drosophila U4atac -- hypothetical *F caataatgttataaataataaacaatttttaatttttagaaggaagtcaaaagtagagtgtaaatcgcttattacacttt *F atttacaaacgatattttagtgtatgcaatatttcccttgc *F *F > Drosophila U6atac -- hypothetical *F gtgttgtttggaaggagagcaagttagcactcccctagacaaggatggaacacataaacggtcggctaggcacagacaaa *F agccgtccacaaatttt *F *F > Drosophila U12 -- hypothetical *F gtgcctcaaactaatgagtaaggaaaaccaatcagccttgctaatcgcttggcagtattggcttctaggcaggggggcgt *F gtcccgcgccccttgaagctcaaatttttgcaagggcacaggtcgtcccctcctcctccgcgtgggtggcgttcggccga *F gcgaaccggcgcctactttgcgtccggctagcgaggatctctgggtgccatcccacggctgggtgttgcgatctgccc *F *F \################################################## *F *F Relevant text from Mount and Salz (manuscript in preparation for a special *F issue of the Journal of Cell Biology): *F *F The Drosophila genome contains multiple copies of the 5 UsnRNAs found in *F the major class of sliceosomes. We found five genes for U1, six genes for *F U2, three genes for U4, seven genes for U5, and three genes for U6. With *F the exception of U4-25F, and the U5 genes (which were previously known *F only by in situ hybridization), these genes had been described previously *F [Lo, 1990; Saba, 1986; Das, 1987; Alonso, 1984; Saluz, 1988]. The variant *F U4-25F gene presumably escaped detection because the predicted RNA has *F only 69% identity with the major form of fly U4 [Saba, 1986] and 68% with *F human U4. This degree of divergence is therefore quite high (human and fly *F U4 share 73% identity), yet the gene is likely to be functional because *F the promoter is conserved and some of the variation includes compensatory *F changes that allow formation of the conserved stem loop structures. *F *F The Drosophila genome also contains a minor class, or U12, introns [Hall, *F 1994; Tarn, 1996] and is therefore likely to contain the U12-type *F spliceosome. Identification of snRNAs for the U12-type spliceosomes was *F difficult because they had not been described previously, and were *F somewhat diverged. However, by modification of the standard parameters for *F blastn (see Methods), it was possible to find one gene for U12, one gene *F for U6atac and one gene for U4atac. These are almost certainly real genes, *F as critical sequences are conserved. In addition, the highly conserved *F snRNA promoter, including a 9/10 or perfect match to the PSE consensus *F TAATTCCCAA approximate 52 nucleotides upstream of the start [Lo, 1990 \#19; *F Jensen, 1998] is present in each case. No U11 gene was found. This may be *F due to divergence beyond what can be detected by blastn searches. However, *F it is striking that the one identified protein component unique to the *F U11/U12 snRNP (and minor spliceosome), the U11 35 kd. subunit [Will, 1999 *F \#20] cannot be found either. In fact, the U11 snRNP may not be required *F for splicing, as its role should be 5' splice site recognition, and the *F highly conserved minor splice site consensus is also complementary to *F U6atac snRNA. Thus, minor class 5 splice sites could be recognized by the *F U6atac snRNA alone, or by an unknown protein that acts during the early *F steps of splicing. This mechanism would be analogous to a situation seen *F in vitro where certain vertebrate introns can be processed in the absence *F of U1 snRNP if the 5 splice sites can be recognized by U6 snRNA *F [Crispino,1996 \#21]. *F *F \################################################## *F *F Stephen M. Mount *F Cell Biology and Molecular Genetics *F H. J. Patterson Hall *F University of Maryland *F College Park, MD 20742-5815 *F *F Phone 301-405-6934 *F FAX 301-314-9081 *F permanent email address sm193@umail.umd.edu *F *F \################################################## *F From smount@wam.umd.edu Fri May 12 19:25:14 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Fri, 12 May 2000 19:25:14 +0100 *F X-Authentication-Warning: rac3.wam.umd.edu: smount owned process doing -bs *F Date: Fri, 12 May 2000 14:30:13 -0400 (EDT) *F From: Stephen M Mount *F To: Michael Ashburner *F Subject: Re: snRNA report - some questions *F MIME-Version: 1.0 *F *F Michael, *F *F Sorry to take so long to reply. I had meant to go back and check all of *F the obscure Flybase snRNA entries (most of which are based on poor data), *F but I haven't finished yet, and I am about to fly to CA for Gerry's 50th. *F *F What follows is as far as I got. *F *F Steve *F *F \################################################## *F *F > Steve .. I am now updating the snRNAs in FB with your data. *F > I have some questions, which I need answered before I actually *F > pass the updates on to the database. *F > *F > 0. You made some other minor changes *F > to the names (ie the cytologies) .. were these on the basis of new evidence ? *F > *F > snRNA:U5:23DE > snRNA:U5:23D *F > snRNA:U5:34AB > snRNA:U5:34A *F > snRNA:U5:35EF > snRNA:U5:35D *F > snRNA:U5:63A > snRNA:U5:63BC *F *F The old evidence was in situ hybridization, and I suspect that some if it *F was fairly inaccurate (see below). The names I assigned were based *F entirely on the sequence. Specifically, on interpolation between nearby *F genes within that same sequence accession (e.g. snRNA:U5:63BC is near *F 'BtbVII,' which was assigned to 63B-C). I recognize that this has it's own *F problems. In this case, the BtbVII is over 50 kb. to the right, and the *F only mapped gene on the adjacent accession, Shab, ='CG1066,' 63A1-63A7,' *F is 130 kb. in the other direction. *F *F > 1. snRNA:U2:39B. & snRNA:U2:40AB. FlyBase had these genes but with NO *F > attached sequence. You have U2-38ABa and U2-38ABb. Do I assume one of *F > them is the U2:39B gene & the other is the U2:40AB gene ? *F *F Probably not. U2-38ABa and U2-38ABb are less than 3 kb. apart and would *F not have been distinguished by the method used (in situ hybridization). *F *F My response to the following questions is the same. The in situs in those *F old papers were just not that good. Remember, snRNAs make short *F probes. Your guess as to which is which is probably better than mine. *F *F > 2. FB had these genes with these accession numbers. Not on your lists: *F > snRNA:U2:84Ca, X04247 *F > snRNA:U2:84Cb, X04245, X04246 *F > *F > 3. FB has snRNA:U4:39B, AQ034021 .. is this your U4:38AB *F > *F > 4. FB has snRNA:U4:40AB, no sequence .. is this your U4:39B *F > *F > 5. FB has U5:39B with no sequence data. Is this your U5:38ABa ? *F > *F > 6. FB has these: *F > snRNA:U2:84Ca, X04247 *F > snRNA:U2:84Cb, X04245, X04246 *F > *F > Not on your list *F > *F > 7. I think I asked you this before: Any idea what these are ? *F > *F > \*a snRNA-a *F > \*z FBgn0003454 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26817 *F > \# *F > \*a snRNA-b *F > \*z FBgn0003455 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26818 *F > \# *F > \*a snRNA-c *F > \*z FBgn0003456 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26819 *F > \# *F > \*a snRNA-d *F > \*z FBgn0003457 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26820 *F > \# *F > \*a snRNA:K2a *F > \*z FBgn0016982 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > \*a snRNA:K2b *F > \*z FBgn0016981 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > \*a snRNA:K8 *F > \*z FBgn0016980 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > \*a snRNA:K9 *F > \*z FBgn0016979 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > *F > *F > \*g is the sequence accession and \*x the reference *F > *F > *F > Thanks for all the help. *F > *F > Michael *F > # *U FBrf0128767 *a Mount *b S. *t 2000.5.30 *T personal communication to FlyBase *u *F >From smount@wam.umd.edu Tue May 30 03:52:01 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 30 May 2000 03:52:01 +0100 *F X-Authentication-Warning: rac5.wam.umd.edu: smount owned process doing -bs *F Date: Mon, 29 May 2000 22:57:23 -0400 (EDT) *F From: Stephen M Mount *F To: Michael Ashburner *F Subject: Re: snRNA report - some questions *F MIME-Version: 1.0 *F *F Michael, *F *F Sorry to be so slow in responding. I think that I explained the basis of *F my naming and addressed all of the U-RNAs last time. The questions I did *F not address are below: *F *F > 7. I think I asked you this before: Any idea what these are ? *F > *F > \*a snRNA-a *F > \*z FBgn0003454 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26817 *F *F AE003241 446-506 is a pretty good match. This same sequence is in *F *F >gb|M21017.1|DRORGAB D.melanogaster 18S, 5.8S 2S and 28S rRNA genes, *F complete, and 18S rRNA gene, 5' end, clone pDm238. *F *F This is 95% identical to the 5' end of 18S rRNA. *F *F > \# *F > \*a snRNA-b *F > \*z FBgn0003455 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26818 *F > \# *F *F This sequence is not in the genome, but it is somewhat similar to U6 *F (82%). Could it just be U6 sequenced badly? E = 0.05. It is not supposed *F to be in the cytoplasm (except transiently). *F *F > \*a snRNA-c *F > \*z FBgn0003456 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26819 *F > \# *F *F This is not in the Celera genome, either, and doesn't resemble anything *F enough for comment. *F *F > \*a snRNA-d *F > \*z FBgn0003457 *F > \*x FBrf0042443 == Arrigo et al., 1985, EMBO J. 4: 399--406 *F > \*g M26820 *F > \# *F *F This is 90% identical to a piece of the mitochondrial genome, *F >gb|U37541.1|DMU37541 *F *F Query: 2 aaattttatatttaaattattatataaaaatgtaactcgagat-aaaaaactt 53 *F ||||||||||||||||||||||||||||||||||| | ||| | |||||| || *F Sbjct: 14648 aaattttatatttaaattattatataaaaatgtaatt-gaggttaaaaaattt 14597 *F *F ------------- *F *F The RNAs in this Wooley paper were not characterized sufficiently for *F positive identification. The only one in GenBank is K5, which is U1 (with *F two mistakes in 131 nucleotides, which is not bad for that methodology). *F *F > \*a snRNA:K2a *F > \*z FBgn0016982 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > \*a snRNA:K2b *F > \*z FBgn0016981 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > \*a snRNA:K8 *F > \*z FBgn0016980 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. Sci. USA 79(22): 6762--6766 *F > \# *F > \*a snRNA:K9 *F > \*z FBgn0016979 *F > \*x FBrf0038653 == gm626.h == Wooley et al., 1982, Proc. Natl. Acad. *F > Sci. USA 79(22): 6762--6766 *F > \# *F > *F *F Stephen M. Mount *F Cell Biology and Molecular Genetics *F H. J. Patterson Hall *F University of Maryland *F College Park, MD 20742-5815 *F *F Phone 301-405-6934 *F FAX 301-314-9081 *F permanent email address sm193@umail.umd.edu *F # *U FBrf0128894 *a Sarov-Blat *b ?. *t 2000.7.17 *T personal communication to FlyBase *u *F Delivery-date: Mon, 17 Jul 2000 14:47:52 \+0100 *F Date: Mon, 17 Jul 2000 10:57:17 \-0700 *F From: lea sarovblat *F To: Chihiro Yamada *F Subject: paper Sarov-Blat et al, 2000, Cell 10(6): 647--656 *F >Dear Dr Chihiro, *F > *F >about your questions on the paper Sarov-Blat et al, 2000, Cell 10(6): *F 647--656 *F 1. to1 is fine. *F >1. ry506 associated to allele. *F >================================ *F > *F >You mention in your paper an allele of to that is associated with the *F >ry506 allele. In order to properly curate your data, we need to *F >assign a unique symbol to this allele. Do you have a preferred symbol *F >FlyBase can use to identify your allele? (e.g. to1, where the *F >indicates superscript). *F 2. the accession number is AF261748 and should come out any day if you *F have problems I can foword you a copy that they sent me. *F >2. to accession number. *F >======================= *F > *F >I have been unable to find an accession number for the to gene in your *F >paper. Could you provide me with this information so that we can *F >place it on the genome *F > *F >Any new information you provide me with will be curated as a personal *F >communication from you and FlyBase, if that is fine with you. *F Please feal free to ask me if you have more questions *F sincerely, *F Lea Sarov-Blat *F \--- *F Dear Dr Rosbash, *F I am currently curating your paper for FlyBase: *F Sarov-Blat et al, 2000, Cell 10(6): 647--656 *F I have a couple of quick questions I was hoping you could answer for me: *F 1. ry506 associated to allele. *F ================================ *F You mention in your paper an allele of to that is associated with the *F ry506 allele. In order to properly curate your data, we need to *F assign a unique symbol to this allele. Do you have a preferred symbol *F FlyBase can use to identify your allele? (e.g. to1, where the *F indicates superscript). *F 2. to accession number. *F ======================= *F I have been unable to find an accession number for the to gene in your *F paper. Could you provide me with this information so that we can *F place it on the genome *F Any new information you provide me with will be curated as a personal *F communication from you and FlyBase, if that is fine with you. *F Best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0128895 *a Brown *b ?. *t 2000.7.20 *T personal communication to FlyBase *u *F Date: Wed, 19 Jul 2000 19:16:02 \+0000 *F To: Chihiro Yamada *F From: Nick Brown *F Subject: Re: FlyBase Query (cy721) *F Hiya Chihiro, *F .. *F To answer your questions: *F what we have called alphaPS4 does correspond approximately to CG16827 *F what we have called alphaPS5 does correspond approximately to CG5372 *F Our predicted alpha subunits are longer than these predicted ORFs at the *F N-terminus *F Our argument for calling them alphaPS's is that they are so similar to *F alphaPS3 we predict they will also form heterodimers with betaPS (mys). *F However there is no biochemical data to support that. As far as naming the *F genetic loci, as I indicated in the review I suspect that scab is a complex *F locus that encodes both alphaPS3 and alphaPS4, but I do not have any data *F to support this yet other then their close proximity and the weak phenotype *F of apparent alphaPS3 molecular nulls. We do not know of any mutants in *F alphaPS5 yet. You could just keep them as CG numbers until the mutants are *F found and the genes named. *F > *F Paxillin is currently annotated as two overlapping genes CG18576 and CG18061 *F We have a different prediction for the gene structure but have not done any *F experiments to confirm it. *F > *F To make the gene encoding the Filamin at 59A, combine exons from CG13525, *F CG3550 and CG11605 going 5' to 3' Again this is based on sequence gazing *F rather than additional data *F >Any new info you give us I'll curate as a personal communication from *F >you to FlyBase, if that's O.K. with you. *F > *F Thats fine. *F .. *F Dr Nick Brown *F Wellcome/CRC Institute *F Tennis Court Road *F Cambridge CB2 1QR *F England *F Tel 44-1223-334128 *F Fax 44-1223-334089 *F You can also reach me by contacting my Assistant Chris Stewart *F e-mail cls23@cus.cam.ac.uk *F Tel 44-1223-334143 (2-6pm GMT) *F To: nb117@mole.bio.cam.ac.uk *F Subject: FlyBase Query (cy721) *F From: Chihiro Yamada *F Date: Tue, 18 Jul 2000 17:11:52 \+0100 *F Content-Length: 2350 *F Hiya Nick, *F .. *F I'm curating a review of yours \- the Dev Biol one: *F Brown et al, 2000, Dev. Biol. 223(1) 1-16 *F I've got a couple of questions I hope you can help me with. *F 1.PS4&5 *F ============== *F You talk about PS4 and PS5. We have in our records 2 CGs *F CG16827, and CG5372, that have been assigned gene product names *F 'integrin, &agr;-subunit'. What you say about the relationship between *F PS4 and scab (on page 3) suggests that PS4 is CG16827 (having *F had a look on Genescene). Can you confirm this? Which leaves by a *F process of elimination PS5 being CG5372. Is this true? Or are *F there other units that FlyBase doesn't know about? *F Secondly these two don't have names (other than CGs) Do they have *F names and symbols yet? If not I'll call them PS4 and PS5 *F (where is the symbol alpha) though this would be a little odd *F as PS1-3 have the mutant names as their Flybase names, with the PS *F names as synonyms. *F 2. Paxillin *F ========== *F I can't find any gene attributed with 'Paxillin' in our records. Do you *F have a CG for this? Or if you don't know can you tell me where you *F found out about this and give me a reference, so I can check it out? *F 3. Filamin 59A *F ============== *F You mention a second filamin (not cheerio) AT 59A. I can't find any *F other genes attributed with 'Filamin' in our records. Do you have a CG *F for this? Or if you don't know can you tell me where you found out *F about this and give me a reference, so I can check this out as well? *F Any new info you give us I'll curate as a personal communication from *F you to FlyBase, if that's O.K. with you. *F Finally, FYI, whilst curating your review , I noticed that you didn't *F know (at the time of writing anyway) that PINCH and steamer duck are in *F fact the same gene. We have that merge attributed to: *F Clark and Beckerle, 2000, A. Dros. Res. Conf. 41: 646C *F Stay cool, say Hi to anyone I know. *F Chihiro, *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0128896 *a Bilder *b D. *t 2000.3.24 *T personal communication to FlyBase *u FlyBase error report for CG5462 on Fri Mar 24 05:06:17 2000. *F From FlyBase-error@whitefly.lbl.gov Fri Mar 24 13:02:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 24 Mar 2000 13:02:24 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 05:06:17 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F cc: nomi@bdgp.lbl.gov *F Subject: FlyBase error report *F Content-Length: 618 *F Error report from bilder@rascal.med.harvard.edu *F Gene or accession: CG5462 *F Missed gene *F Comments: This gene is 'scribble' (AF190774). See Bilder,D. and Perrimon,N. *F Localization of apical epithelial determinants by the basolateral PDZ protein *F Scribble. Nature 403 (6770), 676-680 (2000). I am not familiar with all the *F features of GAD but clearly there are 16 LRRs in the protein N-term which are *F not described. Additionally, the function should include something about *F scrib being a likely component of cell-cell junctions. *F Browser: Mozilla/4.7C-CCK-MCD (C-UDP; EBM-APPLE) (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128897 *a Brodsky *b M.H. *t 2000.3.25 *T personal communication to FlyBase *u FlyBase error report for CG10873 on Sat Mar 25 18:17:37 2000. *F From FlyBase-error@whitefly.lbl.gov Sat Mar 25 02:13:19 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 25 Mar 2000 02:13:19 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 18:17:37 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 573 *F Error report from brodsky@uclink4.berkeley.edu *F Gene or accession: CG10873 *F Missed gene *F Comments: cDNA sequence is currently available in genbank (accession \#s *F AF224713, AF224714). This sequence corrects the peptide sequence and *F intron-exon structure predicted from the genomic. *F References: AF224713, AF224714, *F Michael H. Brodsky, William Nordstrom, Garson Tsang, Elaine Kwan, Gerald M. *F Rubin, and John M. Abrams. (2000). Drosophila p53 Binds a Damage Response *F Element at the reaper Locus. Cell 101, 103-113. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128898 *a Kaplan *b C. *t 2000.3.24 *T personal communication to FlyBase *u FlyBase error report for CG5257 on Fri Mar 24 12:41:21 2000. *F From FlyBase-error@whitefly.lbl.gov Fri Mar 24 20:37:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 24 Mar 2000 20:37:07 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 12:41:21 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F cc: nomi@bdgp.lbl.gov *F Subject: FlyBase error report *F Content-Length: 619 *F Error report from ckaplan@fas.harvard.edu *F Gene or accession: CG5257 *F Gene annotation error *F Genes CG5257 and CG5253 were merged. *F Comments: CG5257 encodes a protein with a ribosomal S1 domain homologous to a *F prokaryotic family of proteins including B. pertussis Tex and several *F hypothetical conserved proteins. CG5253, directly upstream from CG5257 *F encodes a protein homologous to the this same bacterial family, but lacking an *F S1 domain. CG5253 and CG5257 by homology encode contiguous domains of the *F same protein, probably separated by a large intron. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128899 *a Good *b P. *t 2000.3.24 *T personal communication to FlyBase *u FlyBase error report for CG12478 on Fri Mar 24 14:47:04 2000. *F From FlyBase-error@whitefly.lbl.gov Fri Mar 24 22:42:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 24 Mar 2000 22:42:52 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 14:47:04 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 690 *F Error report from pgood@lsumc.edu *F Gene or accession: CG12478 *F Gene annotation error *F Genes CG12478 and CG10046 should be merged. *F Comments: The predicted protein sequence for CG124778 encodes the middle part *F of an RNA-binding protein in the Bruno/CUGBP/Etr family (ie U16800). The *F predicted protein sequence for CG10046, immediately downstream, encodes the *F carboxy terminal half of this protein. The amino terminal domain for this *F protein is still missing from the genomic sequence. The intron/exon *F boundaries and protein sequence similarities to the human genes strongly *F support this relationship (p. good, data not shown). *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128900 *a Good *b P. *t 2000.3.24 *T personal communication to FlyBase *u FlyBase error report for CG6319 on Fri Mar 24 15:44:42 2000. *F From FlyBase-error@whitefly.lbl.gov Fri Mar 24 23:40:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 24 Mar 2000 23:40:30 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 15:44:42 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 693 *F Error report from pgood@lsumc.edu *F Gene or accession: CG6319 *F Gene annotation error *F Gene CG6319 should be split. *F Comments: This gene should be split into two highly related genes of the *F Bruno/CUGBP/Etr family. See Webster et al., Genes & Dev. (FBrf0098905) for a *F definition of the arrest gene. To correct, the exon defined by nt *F 221089..221297 should be extended to 221082..221369 and an additional 3' exon *F at 229333..223208 (see accession U73846 for assignment of 3' utr exon). A *F new gene should start with the next exon. Unfortunately, I have not found any *F EST sequences in the database corresponding to the second gene. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128901 *a Kaplan *b C. *t 2000.3.24 *T personal communication to FlyBase *u FlyBase error report for CG8810 on Fri Mar 24 11:23:51 2000. *F From FlyBase-error@whitefly.lbl.gov Fri Mar 24 19:19:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 24 Mar 2000 19:19:40 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 11:23:51 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F cc: nomi@bdgp.lbl.gov *F Subject: FlyBase error report *F Content-Length: 1111 *F Error report from ckaplan@fas.harvard.edu *F Gene or accession: CG8810, Dspt4 *F Gene annotation error *F Gene Dspt4 has a mistake in the supporting evidence or functional assignment. *F Comments: CG8810 is annotated correctly as a gene on the (-) strand having *F homology to BolA protein, and is a predicted mevalonate kinase. Dspt4 is on *F the (+) strand annotated as the Drosophila homolog of yeast Spt4 (correct) but *F it is also annotated as a mevalonate kinase. The problem seems to lie in the *F overlapping transcription units of these genes. BDGP cDNA LD44495 (5' *F sequence) encodes the entire Dspt4 ORF but overlaps with 3' CK02143 and 3' *F GH04687. LD16567, which encodes part of CG8810, is downstream from Dspt4 on *F the opposite strand. Sequence data from our lab indicates that the LD44495 3' *F end ((+) strand) is downstream from the 5' end of LD16567 ((-) strand). *F Therefore the majority of CG8810 may be transcribed on the opposite strand as *F part of the Dspt4 transcription unit, confusing the annotation. If this *F explanation is unclear, please e-mail me. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Referred by: # *U FBrf0128902 *a Ashburner *b M. *t 2000.3.25 *T personal communication to FlyBase *u FlyBase error report for CG15288 on Sat Mar 25 13:26:19 2000. *F From FlyBase-error@whitefly.lbl.gov Sat Mar 25 21:21:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 25 Mar 2000 21:21:59 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Sat, 25 Mar 2000 13:26:19 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 326 *F Error report from M. Ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG15288 *F Gene annotation error *F Genes CG15288 and wb should be merged. *F Comments: This is clearly wb. Do we also have something identified as wb ? *F Gene model looks screwy. ma *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Referred by: query.pl # *U FBrf0128903 *a Abrams *b J. *t 2000.3.26 *T personal communication to FlyBase *u FlyBase error report for CG4319 on Sun Mar 26 09:58:43 2000. *F From FlyBase-error@whitefly.lbl.gov Sun Mar 26 18:54:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 26 Mar 2000 18:54:23 \+0100 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Sun, 26 Mar 2000 09:58:43 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 2014 *F Error report from John Abrams (abrams@utsw.swmed.edu) *F Gene or accession: CG4319 *F Gene annotation error *F Gene rpr and fas not homologs has a mistake in the supporting evidence or *F functional assignment. *F Comments: The report states that reaper and fas are homologs. This is highly *F unlikely. *F The proposition that RPR might represent an ancestral member of 'death *F domain' containing proteins and, as such, could share functions in common with *F death receptors (Cleveland and Ihle, 1995; Golstein et al., 1995; Golstein et *F al., 1995) was not supported by studies in vivo (Chen et al., 1996; Vucic et *F al., 1997). The lack of a homologous relationship between REAPER and of *F 'death domain' containing proteins was further bolstered by the recent *F structural determination of a death domain (Huang et al., 1996; Liang and *F Fesik, 1997). *F References *F Chen, P., Lee, P., Otto, L., and Abrams, J. M. (1996). Apoptotic activity of *F REAPER is distinct from signalling by the tumor necrosis factor receptor 1 *F death domain. J. Biol. Chem. 271, 25735-25737. *F Cleveland, J. L., and Ihle, J. N. (1995). Contenders in FasL/TNF death *F signaling. Cell 81, 479-82. *F Golstein, P., Marguet, D., and Depraetere, V. (1995). Fas bridging cell death *F and cytotoxicity: the reaper connection. Immunol. Rev. 146, 45-56. *F Golstein, P., Marguet, D., and Depraetere, V. (1995). Homology between Reaper *F and the cell death domains of Fas and TNFR1. Cell 81, 185-186. *F Huang, B., Eberstadt, M., Olejniczak, E. T., Meadows, R. P., and Fesik, S. W. *F (1996). NMR structure and mutagenesis of the Fas (APO-1/CD95) death domain. *F Nature 384, 638-41. *F Liang, H., and Fesik, S. W. (1997). THREE-DIMENSIONAL STRUCTURES OF PROTEINS *F INVOLVED IN PROGRAMMED CELL DEATH Review. Journal of Molecular Biology 274, *F 291-302. *F Vucic, D., Seshagiri, S., and Miller, L. K. (1997). Characterization of *F reaper- and fadd-induced apoptosis in a lepidopteran cell line. Molecular & *F Cellular Biology 17, 667-676. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128904 *a Kunes *b S. *t 2000.3.27 *T personal communication to FlyBase *u FlyBase error report for CG1862 on Mon Mar 27 08:09:42 2000. *F From FlyBase-error@whitefly.lbl.gov Mon Mar 27 17:05:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 27 Mar 2000 17:05:45 \+0100 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Mon, 27 Mar 2000 08:09:42 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 266 *F Error report from Sam Kunes (kunes@fas.harvard.edu) *F Gene or accession: CG1862 *F Missed gene *F Comments: This sequence has been previously posted by GenBank and should be *F referenced as accession AAF28394 *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128905 *a Park *b Y.S. *t 2000.3.27 *T personal communication to FlyBase *u FlyBase error report for CG18105 on Tue Mar 27 15:22:12 2000. *F From FlyBase-error@whitefly.lbl.gov Tue Mar 28 00:17:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Mar 2000 00:17:49 \+0100 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Mon, 27 Mar 2000 15:22:12 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 444 *F Error report from Yoonseong Park (ypark@ucrac1.ucr.edu) *F Gene or accession: CG18105 *F Missed gene *F Comments: CG18105 can be named 'eth', ecdysis triggering hormone, which *F appeared in AF170922 in genbank. Your connection of CG18105 to flybase *F correctly describes eth and genbank accession. Thus, only the correction of *F CG18105 to 'eth' may be enough. *F Thanks. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 95; DigExt) *F Referred by: query.pl # *U FBrf0128906 *a Perkins *b L. *t 2000.3.27 *T personal communication to FlyBase *u FlyBase error report for CG7935 on Mon Mar 27 20:53:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Mar 28 05:48:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Mar 2000 05:48:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 27 Mar 2000 20:53:00 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 566 *F Error report from Liz Perkins (perkins@helix.mgh.harvard.edu) *F Gene or accession: CG7935 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG7935. *F Comments: Congratulations on new genome data \- look great! *F Concerning transcript CT23948 (region CG7935), on 3L at ~66B8: this is *F listed as having homology to RanBP7 (aka Importin 7) which it does. This *F gene is already in flybase under the gene name DIMinished-7 (dim-7). Ref: *F Lorenzen et al., 1999. *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Referred by: query.pl # *U FBrf0128907 *a Leiserson *b W.M. *t 2000.3.28 *T personal communication to FlyBase *u FlyBase error report for CG7693 on Tue Mar 28 11:51:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Mar 28 20:47:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Mar 2000 20:47:36 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Mar 2000 11:51:42 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 597 *F Error report from William M. Leiserson (william.leiserson@yale.edu) *F Gene or accession: CG7693 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG7693. *F Comments: This gene (listed as Ste20-like) has been named fray and is listed *F in FlyBase. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 98; DigExt) *F Referred by: query.pl # *U FBrf0128908 *a Perkins *b L. *t 2000.3.28 *T personal communication to FlyBase *u FlyBase error report for CG3954 on Tue Mar 28 14:20:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Mar 28 23:16:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Mar 2000 23:16:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Mar 2000 14:20:57 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 317 *F Error report from Liz perkins (perkins@helix.mgh.harvard.edu) *F Gene or accession: CG3954 *F Missed gene *F Comments: The gene CG3954 is the gene Corkscrew (csw) *F The transcript CT10363 is csw Y1229 *F The transcript CT37554 is csw 4A *F Thanks! *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Referred by: query.pl # *U FBrf0128909 *a Burmester *b T. *t 2000.3.29 *T personal communication to FlyBase *u FlyBase error report for CG11538 on Wed Mar 29 01:48:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Mar 29 10:44:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 29 Mar 2000 10:44:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 29 Mar 2000 01:48:37 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 424 *F Error report from Thorsten Burmester (thorsten@uzomai.biologie.uni-mainz.de) *F Gene or accession: CG11538 *F Gene annotation error *F Gene CG11538 corresponds to FBgn0017424 *F Comments: anon-68Ed is LSP-2. As explained in Mousseron-Grall et al. (1997), *F the long *F anti-sense ORF of LSP-2 (i.e. anon-68Ed) does not code for any gene. The *F actual gene *F and transcript is LSP-2. *F Browser: Mozilla/4.7 en (Win95; I) *F Referred by: query.pl # *U FBrf0128910 *a Artero *b R. *t 2000.3.29 *T personal communication to FlyBase *u FlyBase error report for CG7449 on Tue Mar 28 17:54:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Mar 29 02:50:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 29 Mar 2000 02:50:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Mar 2000 17:54:39 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 630 *F Error report from Ruben Artero (r-artero@ski.mskcc.org) *F Gene or accession: CG7449 *F Gene annotation error *F Gene CG7449 corresponds to AF210316 (FBgn0029082) *F Comments: The sequence of a transcript from gene CG7449 has already been *F reported (see accesion number AF210316; mRNA from gene 'hibris') which gives a *F slightly larger protein (1235 aa) because of the use of an upstream exon *F approximately 24 kb from the first exon annotated in CG7449. Transcription *F size is 6.2 kb (experimental data). A proper link should be made to flybase *F gene FBgn0029082 ('hibris' gene). *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128911 *a Spring *b J. *t 2000.3.29 *T personal communication to FlyBase *u FlyBase error report for CG18144 on Wed Mar 29 02:31:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Mar 29 11:27:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 29 Mar 2000 11:27:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 29 Mar 2000 02:31:28 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1054 *F Error report from Jurg Spring (J.Spring@unibas.ch) *F Gene or accession: CG18144 *F Gene annotation error *F Gene CG18144 has incorrect exon/intron structure. *F Comments: The region reported to code for the 15 aa gene product CG18144 *F contains the *F bHLH (basic helix-loop-helix) Hand gene, equally similar to human paralogues *F HAND1 and HAND2. As predicted by FGenes, using one of the CG18144 splice sites, *F a 174 aa protein is assembled from 4 exons, with a poorly conserved (but similar *F size) N-terminus, a highly conserved bHLH domain and a highly conserved *F C-terminal *F Hand-specific second motif (KGRTWWPxVWAxEL). *F Predicted protein: *F >FGENES *F MFKNSVALTCEYSTMYYNSIYNTSNMFDMKHSESQVQQQIYNTSHLGYVPTSNTRIVKKR *F NTANKKERRRTQSINNAFSYLREKIPNVPTDTKLSKIKTLKLAILYINYLVNVLDGDLDP *F KGGFRAELKPVSRKICSEKKHCLKSEIQNVPLSTKGRTGWPQDVWASELIPEHN *F from AE003628: complement *F 1 \- 1 CDSl 55866 \- 55946 *F 1 \- 2 CDSi 57559 \- 57714 *F 1 \- 3 CDSi 57948 \- 58144 *F 1 \- 4 CDSf 58294 \- 58384 *F Kind regards, *F Jurg Spring *F Browser: Mozilla/4.5 en (WinNT; I) *F Referred by: query.pl # *U FBrf0128912 *a Brody *b T. *t 2000.3.30 *T personal communication to FlyBase *u FlyBase error report for CG6890 on Wed Mar 29 17:13:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 30 02:09:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Mar 2000 02:09:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 29 Mar 2000 17:13:55 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 236 *F Error report from Thomas Brody (brodyt@codon.nih.gov) *F Gene or accession: CG6890 *F Missed gene *F Comments: misclassification \- its a kinase not a gpcr *F Browser: Mozilla/4.0 (compatible; MSIE 4.01; MSN 2.5; Windows 98) *F Referred by: query.pl # *U FBrf0128913 *a Boehm *b S. *t 2000.3.30 *T personal communication to FlyBase *u FlyBase error report for CG5086 on Thu Mar 30 02:12:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 30 11:07:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Mar 2000 11:07:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 30 Mar 2000 02:12:18 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 479 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG5086 *F cDNA or EST error *F Comments: This ORF/gene is annotated as C2H2-zinc finger protein (5-6 fingers). *F In the aa_gadfly this entry FBan0005086|CT16303 with 517aa does not have any *F fingers.It seems to me that perhaps the C-terminal half (the second exon) is *F missing in the aa-gadfly entry.Is this the case ? *F bw Siegfried *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 95) *F Referred by: query.pl # *U FBrf0128914 *a Gindhart *b J. *t 2000.3.30 *T personal communication to FlyBase *u FlyBase error report for CG11059 on Thu Mar 30 06:28:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 30 15:23:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Mar 2000 15:23:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 30 Mar 2000 06:28:19 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 738 *F Error report from Joe Gindhart (joseph.gindhart@umb.edu) *F Gene or accession: CG11059 *F Missed gene *F Comments: Another close homolog of gene CG11059 is locus BAA34446, which *F corresponds to Homo sapiens KIAA0726 protein. The Score for the CG11059 vs. *F KIAA0726 comparison is 296, with an E value = 4e-80. *F Here's the information pasted *F from its Entrez entry: *F LOCUS BAA34446 968 aa PRI 16-JUN-1999 *F DEFINITION KIAA0726 protein Homo sapiens. *F ACCESSION BAA34446 *F PID g3882173 *F VERSION BAA34446.1 GI:3882173 *F DBSOURCE locus AB018269 accession AB018269.1 *F KEYWORDS . *F SOURCE human. *F Browser: Mozilla/4.06 enC-compaq (Win98; I) *F Referred by: query.pl # *U FBrf0128915 *a Kelley *b R. *t 2000.3.31 *T personal communication to FlyBase *u FlyBase error report for CG2913 on Thu Mar 30 15:46:38 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Mar 31 00:42:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 31 Mar 2000 00:42:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 30 Mar 2000 15:46:38 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 544 *F Error report from Rick Kelley (rkelley@bcm.tmc.edu) *F Gene or accession: CG2913 *F Missed gene *F Comments: The gene you have labelled 'yin' should be named 'opt1'. Yin was *F used only in abstracts before the function was determined. All peer reviewed *F publications refer to the gene as opt1. *F There is a noncoding RNA gene immediately 3' of opt1. The roX1 gene produces *F a ~3.7 kb noncoding RNA which is a component of the dosage compensation *F complex which coats the male X chromosome. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Referred by: query.pl # *U FBrf0128916 *a den Dunnen *b J. *t 2000.4.1 *T personal communication to FlyBase *u FlyBase error report for CG4976 on Sat Apr 1 05:55:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 01 14:50:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 1 Apr 2000 14:50:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 1 Apr 2000 05:55:00 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 527 *F Error report from Johan den Dunnen (ddunnen@lumc.nl) *F Gene or accession: CG4976 *F Missed gene *F Comments: The current annotation is; Drosophila NSD1 homolog (CT15944 product). *F It should be; Drosophila WHSC1 homolog (CT15944 product). *F The WHSC1 (MMSET) protein has over its full length a clear homology with *F this Drosophila, which is not true for the NSD1 protein. WHSC1 was *F published by Stec I et al. in Hum.Mol.Genet. 1998 7(7):1071-1082 *F Browser: Mozilla/4.7 en (Win98; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128917 *a Escher *b S.A. *t 2000.4.5 *T personal communication to FlyBase *u FlyBase error report for CG17975 on Wed Apr 5 06:00:23 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 05 13:55:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Apr 2000 13:55:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 5 Apr 2000 06:00:23 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 699 *F Error report from Stefan A Escher (stefan.a.escher@genetik.umu.se) *F Gene or accession: CG17975 *F Gene annotation error *F Gene CG17975 has incorrect exon/intron structure. *F Comments: We call this gene sut2, (it belong to the sut1,2 and 3 complex) and *F we have sequenced the corresponding EST (GH25507) and the sequence differ from *F your predicted sequence. *F 1. one exon is spliced differently, compare to our sequence AF199484 *F 2. we have predicted that the transcription start upstreams of yours, since *F this gives high homology to sut1 and sut3 in this part of the gene. *F All the best, *F Stefan *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 95; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128918 *a Escher *b S.A. *t 2000.4.5 *T personal communication to FlyBase *u FlyBase error report for CG17976 on Wed Apr 5 06:13:59 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 05 14:09:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Apr 2000 14:09:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 5 Apr 2000 06:13:59 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 476 *F Error report from Stefan A Escher (stefan.a.escher@genetik.umu.se) *F Gene or accession: CG17976 *F Missed gene *F Comments: We call this gene sut3 \- AF199484. *F It belong to the sugar transporter complex sut1, 2 and 3. This are sugar *F transporter genes with high similarity. *F We are looking into the other transcripts with sugar transporter motifs. *F All the best, *F Stefan *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 95; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128919 *a Nairz *b K. *t 2000.4.5 *T personal communication to FlyBase *u FlyBase error report for CG9936 on Wed Apr 5 09:56:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 05 17:52:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Apr 2000 17:52:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 5 Apr 2000 09:56:31 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 348 *F Error report from Knud Nairz (nairz@zool.unizh.ch) *F Gene or accession: CG9936 *F Gene annotation error *F Gene CG9936 corresponds to AF226855 *F Comments: Gene CG9936 corresponds to genbank-entry AF226855 coding for *F 'flytrap', the Drosophila homolog of TRAP240. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128920 *a Nairz *b K. *t 2000.4.5 *T personal communication to FlyBase *u FlyBase error report for CG9936 on Wed Apr 5 10:01:06 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 05 17:56:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Apr 2000 17:56:37 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 5 Apr 2000 10:01:06 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 427 *F Error report from Knud Nairz (nairz@zool.unizh.ch) *F Gene or accession: CG9936 *F Gene annotation error *F Gene CG9936 has incorrect exon/intron structure. *F Comments: The complete cDNA-sequence of gene CG9936 \- also called 'flytrap' *F \- has been determined (AF226855). The intron-exon structure predicted by *F genefinder is not entirely correct. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128921 *a Nairz *b K. *t 2000.4.5 *T personal communication to FlyBase *u FlyBase error report for CG9936 on Wed Apr 5 10:02:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 05 17:58:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 5 Apr 2000 17:58:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 5 Apr 2000 10:02:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 369 *F Error report from Knud Nairz (nairz@zool.unizh.ch) *F Gene or accession: CG9936 *F Gene annotation error *F Gene CG9936 has a mistake in the supporting evidence or functional assignment. *F Comments: Gene CG9936 \- also called 'flytrap' (AF226855) \- is a coactivator, *F but not a receptor. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128922 *a Peifer *b M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG7340 on Thu Apr 6 10:30:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 06 18:26:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 6 Apr 2000 18:26:26 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 10:30:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 5142 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG7340 *F Missed gene *F Comments: We have deposited the complete coding sequence of this gene, a *F putative cytosolic aminopeptidase, in GenBank AF218812 *F The corresponding amino acid sequence is in AAF32329. *F The entry is below. *F AF218812 . Drosophila melanog...gi:6942154 Protein *F LOCUS AF218812 1938 bp mRNA INV 09-FEB-2000 *F DEFINITION Drosophila melanogaster clone SD04712 putative cytoplasmic *F aminopeptidase mRNA, complete cds. *F ACCESSION AF218812 *F VERSION AF218812.1 GI:6942154 *F KEYWORDS . *F SOURCE fruit fly. *F ORGANISM Drosophila melanogaster *F Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; *F Muscomorpha; Ephydroidea; Drosophilidae; Drosophila. *F REFERENCE 1 (bases 1 to 1938) *F AUTHORS Kirkpatrick,C. and Peifer,M. *F TITLE A putative cytoplasmic aminopeptidase from Drosophila *F JOURNAL Unpublished *F REFERENCE 2 (bases 1 to 1938) *F AUTHORS Kirkpatrick,C. and Peifer,M. *F TITLE Direct Submission *F JOURNAL Submitted (23-DEC-1999) Biology, University of North Carolina, *F Coker Hall, CB 3280, Chapel Hill, NC 27599-3280, USA *F FEATURES Location/Qualifiers *F source 1..1938 *F /organism='Drosophila melanogaster' *F /db_xref='taxon:7227' *F /clone='SD04712' *F CDS 96..1772 *F /codon_start=1 *F /product='putative cytoplasmic aminopeptidase' *F /protein_id='AAF32329.1' *F /db_xref='GI:6942155' *F /translation='MRLLLCVQAIRNHLARTYSSARFKKMATDVKFNESLGCSDPQTH *F PVLIIGQLRHLNLLKFSHLETKLSPRVTEETFLNAVACLHPEPTDKVSLYLDVATVAA *F LPLKASRHNTASRAHAITRLVKNHVLNVSEESVVLVCERENLFASACAVVRAFPLYSR *F KTGNLLASSQPKLNLGCGDGNANSGRNVVNVEFVLINKDGCIESEPLTDDELNCLNET *F TRAIRMTARIVDMPCNEMNVDHFIQEVEDVGRELCITPKVIRGEELLEQGFGGIYGVG *F KAAAVPPALVVLSHEPKGAQETIALVGKGIVYDTGGLSIKAKTGMPGMKRDCGGAAAI *F LGTFYAAVQCGFRDNLHAVFCLAENSVGPNATRPDDIHTLYSGRTVEINNTDAEGRLV *F LADGVCYANKDLKANIILDMATLTGAQGVATGKYHGAILTNSETWEAKSLQAGRKSGD *F LLAPIIYCPELHFSEFASAIADMKNSVADRQNAQSSCAGLFIAAHLGFDYPGIWMHVD *F MATPVHCGERATGYGVALLLTLFGGHTDSKLLQSIASTDEEPPSKRWCRD' *F BASE COUNT 474 a 524 c 500 g 440 t *F ORIGIN *F 1 aaaaaattgc aagtcaaatt ggcagcaaaa taaaataaaa ataaaacgtc ggcacttttg *F 61 gcaaattttt gtcaaatttc tgaagcaggc acttcatgcg tttgctgctc tgcgtccagg *F 121 caatacgcaa ccatctcgct cgcacctaca gcagcgcccg cttcaaaaag atggccacgg *F 181 acgtaaagtt taacgagtcg ctaggctgca gtgatccgca gacacatccg gtcctgatca *F 241 tcggccagct gcgccatcta aacctactga agttcagtca tctggagacc aaactcagtc *F 301 cgcgcgtcac cgaggagacc ttcctgaatg ccgtcgcttg tctgcatccc gagcccaccg *F 361 ataaggtatc cctctatctc gatgtggcca ccgtggctgc cctgccattg aaggcatccc *F 421 gacacaatac ggcatcccgg gctcatgcca tcactcgtct ggtaaagaac catgtgctga *F 481 acgtttccga ggagagcgtg gtgctcgtct gcgaacgaga gaatctattc gccagcgcct *F 541 gtgcggtggt tcgcgctttt ccactctatt cccgcaaaac gggcaatctg ctggcctcaa *F 601 gtcagccaaa gctaaatctt ggatgtggag atggaaacgc aaattcagga cgcaacgtag *F 661 tcaatgttga gtttgtgcta atcaacaagg atggctgcat tgagagcgag cctttgacag *F 721 acgacgagct aaattgcctg aatgagacca cacgggcaat tcgaatgact gctcgcattg *F 781 tagatatgcc ctgcaacgaa atgaacgtgg accacttcat ccaggaagtt gaggatgtgg *F 841 gcagggagct gtgcattacg ccaaaagtaa tccgtggcga ggagctgctg gagcaaggat *F 901 ttggcggcat ttacggcgtg ggcaaagctg cggctgtgcc gcctgcccta gttgtgctct *F 961 cccatgagcc aaagggcgcc caggagacta tcgctctggt cggcaagggc atcgtctacg *F 1021 acaccggtgg cctcagcatc aaggccaaga ccggcatgcc cggcatgaag cgcgattgcg *F 1081 gcggagcggc ggcgattctc ggaacctttt acgcggctgt ccagtgtgga tttagggata *F 1141 acttgcatgc cgtcttctgc ttggcggaga actctgtcgg accaaatgcc actcgccccg *F 1201 atgacattca caccctttac tcgggccgca cagtggagat caataacacg gatgccgagg *F 1261 gtcgcttggt gctcgccgat ggcgtttgct acgccaacaa ggatctgaag gccaacatta *F 1321 ttctcgacat ggccacattg actggagcac agggcgttgc aacaggcaaa tatcatggtg *F 1381 ccatattgac aaactcggag acatgggagg caaagtcgtt gcaggctgga cgcaaatctg *F 1441 gcgatttatt ggcacccata atttattgcc ccgaattgca tttctcggaa ttcgcttcgg *F 1501 ccattgctga tatgaaaaac tcggtggcgg atcgtcagaa cgcccaatcc tcctgcgccg *F 1561 gccttttcat tgccgcccat cttggcttcg attatcccgg catctggatg cacgtcgata *F 1621 tggccacgcc cgttcattgt ggggagcgtg ccactggata cggcgttgcc ctgttgttga *F 1681 ccctcttcgg cgggcacact gactccaagc tgcttcaatc catcgcctcc acagacgagg *F 1741 agccgccctc gaagcgctgg tgccgcgatt aactcagcct gccttttact gtgccacgtg *F 1801 cctgactgga ttaacatttc catcattttc tttgcccaat tgcaatccgg catcagaagt *F 1861 caatttgtgt agccgacagg cattaaaaaa tcaatattat ttagaaatac aagaagtatg *F 1921 aaatttaaaa aaaaaaaa *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128923 *a Peifer *b M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG17484 on Thu Apr 6 10:46:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 06 18:41:41 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 6 Apr 2000 18:41:41 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 10:46:02 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 327 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG17484 *F Missed gene *F Comments: This represent the gene p120ctn (see Flybase for references). *F Our Genbank accession number is AF220496 *F Protein AAF33245 *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128924 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG17284 on Thu Apr 6 20:56:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 04:52:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 04:52:27 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 20:56:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 886 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG17284 *F Gene annotation error *F Gene CG17284 has incorrect exon/intron structure. *F Comments: This is a member of a small family of 13 OBP-like proteins (that is *F related to the odorant binding proteins) I have recognized in the genome *F sequences. These are secreted proteins, and the annotated protein does not *F have a signal peptide. By using a slighly 5' exon *F (ATGTATGTGTATAATCTGCTTTTCGTTGTAATAGTTTTTAGCTATTGTGCAAAATCCTTTAATTATACAAGCTGCGAT *F CACGCAAAGCAGCCAAAGTTT) and then including the current 5' end as a phase 0 *F intron (gtatgaaaacttcaaataaaatcgtgtttttattactacaactgaatatttggcag), the encoded *F protein now has a reasonable signal peptide. This intron placement is also *F shared by many of the other members of the family. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128925 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG13518 on Thu Apr 6 21:03:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 04:58:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 04:58:45 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:03:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 777 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13518 *F Gene annotation error *F Gene CG13518 has incorrect exon/intron structure. *F Comments: This is a member of a small family of 13 OBP-like proteins *F (resembling the odorant binding proteins) I have recognized in the genome *F sequences. They are secreted proteins and as annotated this member does not *F have a signal peptide. Addition of a 5' exon *F (ATGCTGAGAATTGGTTTCGTGATTTGTGTGATTATATCACTGCGCTTG) and intron *F (gtacgtactcataacaccgttttcaatttgttctggattggataatgttcgagggtgtttttaatggattatccacac *F tagatcggatttattgtacataataattttgaaagttcaaggtccatcggggatccgtattcgagctactttttaatcc *F tttaatatgtgcatatttctatgcag) provides one. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128926 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG12905 on Thu Apr 6 21:07:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:03:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:03:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:07:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 794 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12905 *F Gene annotation error *F Gene CG12905 has incorrect exon/intron structure. *F Comments: This is a member of a small family of OBP-like proteins (13 total; *F odorant binding proteins) I have recognized in the genome. They are secreted *F proteins, and the current annotated protein does not have a signal peptide. *F Inclusion of a 5' exon *F (ATGTGCTCCCAACTGTTCGCATTTCTGCTCCTCCTTTTGACCGCTTTTGTGACGGGTAGAAGTACTCCACCGGCGTTG *F GATGAAGACTGTGAACTGAATTCTGTAGATACAATG) and intron *F (gtgagttgtagacatggatggatttagagcagtatagaaacgaaagatgcgaaaatcagaataattatagcaaacaaa *F tagaactcgagagccagacaatgaaatcgtcatataaaatgatgatcgggtacaactttttattgca) provides *F one. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128927 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG13939 on Thu Apr 6 21:17:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:12:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:12:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:17:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 649 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13939 *F Gene annotation error *F Gene CG13939 has incorrect exon/intron structure. *F Comments: This is one of 13 members of a newly recognized OBP-like family *F (odorant binding proteins). They are all secreted proteins, and this one is *F missing a signal peptide, which is easily provided by inclusion of a new 5' *F exon *F (ATGCATAAATATATAATTTGTTTTGGTTTTTTACTAATTATTCTGGAATGTTCGTTGGCGTCATTTAACTGCTCAGCA *F CCGCCGAATTTCAATAACTT) and intron *F (gtaagaggaattaatcgtcgaagagtgcatgtaatataaactcaatcttgta). *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128928 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG13940 on Thu Apr 6 21:20:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:16:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:16:23 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:20:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1239 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13940 *F Gene annotation error *F Gene CG13940 should be split. *F Comments: The final exon of this annotated gene encodes most of an OBP-like *F protein, one of 13 I have recognized in the genome. I would recommend *F splitting the current annotation, and including a new 5' exon to provide a *F signal peptide for this secreted protein. My cDNA is as *F follows:ATGTCTTCGGTGCTGCATCTACTGGGTTTCTTGTGGCTTCCTTTGCTGGTTTATAGTGTATCCAATGATAT *F GGGAGGATTGCAGAAATGTACAGAACTTCTAAATACACATAAGTTGGTCTACTGTTGTGGCAAATCCTTTCTGGATAAG *F TTCCCCTTTGTTGGCAGCAATTGCACACCGTTTTGGGATGACTATGGTCCTTGCCGCTATGAATGTCTGTATAGGCACT *F GGGACCTCCTTGATCAGGATAACAAGATCAAAAAACCGGAGCTCTACCTGATGATCACCAGCCTCTACAGCCCCTTGAA *F TGGTTATGATAAATACGGGGCCGCTTTTAAGGCAGCTCACGAGACTTGCGAAGCTCTGGGCTCCAGACACGCCGACTTT *F CTGCTGCTCTACTCCAACCAGGTGGCGGATAAGATGGGCATGGCTTCCTCAACCTGTCTGCCATATGCCATGCTTCATG *F CACAGTGTACCATGGTATACCTAACCGCCAACTGTCCCCGTGAGAACTGGATAGATGATCCGAAGTGCAATAGTCTGCA *F AAAATTGCTGTCAAGTTGCACAAAGAAATTGGACGAAAAGACGAATGCACTTAAGGGAAAGGATGAGGAACTAACGGAC *F AACGGGTGTGGGCATATAGATTCAGAAGGATCCAATCTGCTCATGGCCTGTTTTCTCACACTGATGATCGCCAAGTTCA *F T! *F ATCGGATCATTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128929 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG8769 on Thu Apr 6 21:14:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:19:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:19:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:14:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1287 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG8769 *F Gene annotation error *F Gene CG8769 should be split. *F Comments: This is a member of a small family of OBP-like proteins (13 members; *F odorant binding protein-like) I have recognized in the genome sequences. I *F suggest two changes. First, the cDNA as predicted is correct and needs to be *F translated from nearer the 5' end. Second, the long third intron is probably *F not correct for several reasons. First, it is poorly predicted. Second, it *F is very long while almost all the introns in these proteins are very short. *F Third, this is the only member of the family predicted to have a long *F C-terminus. Fourth, this C-terminal region has a good EST match, which none *F of the others in the family have. I would recommend ending the OBP-lik *F protein shortly into the third intron, and then splitting the remainder off as *F a separate gene. My amino acid translation of the OBP-like protein is *F MLSKSQLLLLVVGFCLNAAVSADVDCSKRPSFVNPKTCCPMPDFVTAELKQKCIKFDMTPPPPPDGEASGSFESKRRHHH *F PHPPPCFFSCIFNETGIYQNRKLDEAKLNAYLQEVFEDSSDLQTTATQAFTTCATKVADFEANLPPRPAPSPPPGFPMCP *F HDAGHLMGCVFRNMMKNCPDSIRNDSQQCTDMKEFFTKCKPPRGPPPSAEDIVRHPRKILFVZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128930 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 6 21:24:40 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:20:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:20:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:24:40 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1134 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: New OBP-like protein *F Missed gene *F Comments: Near CG13939 there is another member of the small (13 member) family *F of OBP-like proteins I have recognized in the genome. My predicted cDNA is *F ATGGCCCGGCATATAGCCCTGCTGATCTGCTCATTGCTAGCGATGGCTGGCTGTGATCCAATCGATGTGGACTGTACTC *F GCAGACAGGATTTCAATATTGTCAAGGACTGCTGTGTCTATCCCACATTTAGGTTTGACCAGTTCAAAAGCCAGTGTGG *F TAAATATATGCCAGTTGGTGCTCCCAGAATTTCACCCTGCCTCTATGAATGCATTTTCAATAAGACCAACACAGTTGTG *F GACGGAGCTATTCATCCTGACAATGCCCGACTCATGCTGGAGAAGCTTTTCGGTAATCAGGACTTTGAAGAAGCCTATT *F TCAATGGTTTAATGGGCTGTTCGGATTCTGTGCAAGAGATGATTAGCAACAGGAGGTCACGGCCCCAAAGAAAAACAGA *F ACAATGCTCTCCATTCTCACTTTTCTATGGAATTTGTGCCCAGAGATATGTCTTCAACCATTGTCCATCATCCAGCTGG *F TCCGGCACTGAATCTTGCGAAATGGCCCGATTGCAGAACATGAACTGTTCGAAACCATCACGTGGTTCTAGTCATCGCC *F TTTAA *F and the encoded protein is *F MARHIALLICSLLAMAGCDPIDVDCTRRQDFNIVKDCCVYPTFRFDQFKSQCGKYMPVGAPRISPCLYECIFNKTNTVVD *F GAIHPDNARLMLEKLFGNQDFEEAYFNGLMGCSDSVQEMISNRRSRPQRKTEQCSPFSLFYGICAQRYVFNHCPSSSWSG *F TESCEMARLQNMNCSKPSRGSSHRL *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128931 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 6 21:27:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:22:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:22:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:27:08 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1107 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: Second new OBP-like protein near CG13939 *F Missed gene *F Comments: Near CG13939 there are three additional unannotated OBP-like *F proteins. This is the second of these. My predicted cDNA is *F ATGCGGACAGGGCGTATACTTGTTGCTTTGATTTTTCTAGGTTTAATAATTCCCTTTCGGGCTGCAAAGTGCAGGGCAG *F CGCCCAAATCTGTGCAAAATGTACACGTTTGTTGCTCAGCACCCCTGCCTAACTGGGGAGTTTTCAACAGAGAGTGTCA *F TAAATCGGCCATTCAAGCAAGTTGCCGTTTAGATTGCGATTTCAATGCCAGCTCGGTTCTGCAGGGAAATCGATTGATC *F CAAGCGAAAGTTCGACCCATGTTGGAGCGCGCCTTTTCCAACGAACCCACCATCGATGCATATGAGTCCAACTTTGCCA *F AATGTTCAACGGTGGTCAGGAGCAAATACCAGGAGCTATCTCCGCTGAGTCGTCAAAGCGACGCCTGTGACAGACACGC *F CCTCTTCTACAGCCTTTGTGCGTATGCTCGCTTGATTTTCACCTGTCCGGACAAAATGTGGCAAAGAAATAACAGGATG *F TGCCAGGAGGCCAAGGCCTATGCGAAAAAATGTCCTTGGCCAGCGCTAAAAATGTTTATGAGGAATACCTAA *F My translation is *F MRTGRILVALIFLGLIIPFRAAKCRAAPKSVQNVHVCCSAPLPNWGVFNRECHKSAIQASCRLDCDFNASSVLQGNRLIQ *F AKVRPMLERAFSNEPTIDAYESNFAKCSTVVRSKYQELSPLSRQSDACDRHALFYSLCAYARLIFTCPDKMWQRNNRMCQ *F EAKAYAKKCPWPALKMFMRNT *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128932 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 6 21:29:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:25:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:25:23 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:29:54 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1083 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: Third new OBP-like gene near CG13939 *F Missed gene *F Comments: This is the third of three new OBP-like proteins I recognize near *F CG13939 in this new small family of 13 proteins. My predicted cDNA is *F ATGCTTCACAAGCTGACTTGGGTTCTAATATTTATACCTGCTTTTCGAGCTGCCGATCCCATTTGCTCTCAAAGGCCGG *F ATGCCTTGAGAAACTGTTGCAAGCTGCCTAATCTGGACTTTTCGAGTTTCAACTCCAAGTGCAGTCAGTATTTGGTCAA *F TGGAGTCCACATATCACCTTGCAGCTTTGAGTGCATCTTTCGAGCGGCGAATGCATTAAATGGCACCCATTTGGTTATG *F GAAAATATCGAAAAGATGATGAAAACCATTTTGGGCTCTGATGAATTTGTGCACGTGTATCTGGATGGATTCAGGAGCT *F GCGGTAATCAGGAGAAGGTGCTCATTAAGGCAATGAAACGTCGCCGTGTTCCCATCACCGGAAAATGTGGCTCGATGGC *F GATAATGTATGGCCTGTGTGCCCATCGATATGTCTACCGTAATTGCCCGGAAAGCGTGTGGAGCAAGAGTGCCACCTGC *F AATGAGGCCAGGGAATACAGCATTCGCTGCGATGACATGTAA *F My predicted protein is *F MLHKLTWVLIFIPAFRAADPICSQRPDALRNCCKLPNLDFSSFNSKCSQYLVNGVHISPCSFECIFRAANALNGTHLVME *F NIEKMMKTILGSDEFVHVYLDGFRSCGNQEKVLIKAMKRRRVPITGKCGSMAIMYGLCAHRYVYRNCPESVWSKSATCNE *F AREYSIRCDDM *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128933 *a Robertson *b H.M. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG11732 on Thu Apr 6 21:34:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 05:29:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 05:29:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 21:34:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1174 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG11732 *F Gene annotation error *F Gene CG11732 has incorrect exon/intron structure. *F Comments: This is one of 13 members of a newly recognized family of OBP-like *F proteins (odorant binding protein). The current annotation is a single ORF, *F and by alignment with the other members of the family I am able to add an *F additiona 5' exon as in the following cDNA *F GACCTATCCATTAAATGCTGCCAGCTAACACGTCCCAGTTTAGATAAAGGCAATTCCGAATGTAGGAAAAGCCTCAACT *F TGCCTGCGCACCGGAAATTCAACTTTGCCGAACTATATACGATTAATATGTGCATTGAGGAGTGCAACTTCATAGGCTG *F TGGCTACATCGAAATCGATCCACCCTTTCGCTTGGATCTGGCCAACATTCGCACCAATCTGCAGACGATTGCACCCCAG *F CCACAGAATGAATCGATACCATTCCTGGTGGATGCGTATCGGAAATGCGAGCTATTCCGATCGTCGCACGGAAGACGAT *F TCACTCTCCACCTGCCAGATATTGAATTCATCGAGGAACCCTGCAATCCCTTTGCCTTACAAATCACCATATGCGTCCG *F CATCCATGCCATGCAAAAGTGTCCCAGCGAATTTTACGTGGACAGCGACGAGTGCCGATTGGCCAGGGAATACTTCACC *F CAGTGCGTCGGGGATATTGAAACCAATCTTGCCTAG *F Nevertheless, I have been unable to find the expected additional 5' exon that *F should encode about 30 amino acids, including a signal peptide. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128934 *a Goldstein *b L. *t 2000.4.7 *T personal communication to FlyBase *u FlyBase error report for CG12192 on Fri Apr 7 08:52:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 16:47:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 16:47:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 7 Apr 2000 08:52:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 347 *F Error report from larry goldstein (lgoldstein@ucsd.edu) *F Gene or accession: CG12192 *F Missed gene *F Comments: there is something wrong with translation. the predicted protein is *F far too short and is clearly missing relevant homologous domains to kinesins. *F Browser: Mozilla/4.51 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128935 *a Stewart *b M. *t 2000.4.7 *T personal communication to FlyBase *u FlyBase error report for CG10944 on Fri Apr 7 15:12:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 23:08:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 23:08:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 7 Apr 2000 15:12:43 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 515 *F Error report from Mary Stewart (mastewar@badlands.nodak.edu) *F Gene or accession: CG10944 *F Gene annotation error *F Gene RpS6 has incorrect exon/intron structure. *F Comments: Comparison of the published cDNA sequence and genomic sequence for *F RpS6 shows *F that the RpS6 gene has three exons that are transcribed. *F The genomic sequence shows that two potential alternate exons may exist *F for RpS6. See Genbank submission L02074 *F Browser: Mozilla/4.07 en (Win95; I ;Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128936 *a Tittiger *b C. *t 2000.4.10 *T personal communication to FlyBase *u FlyBase error report for CG16796 on Mon Apr 10 15:38:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 10 23:34:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 10 Apr 2000 23:34:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 10 Apr 2000 15:38:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 538 *F Error report from Claus Tittiger (crt@unr.edu) *F Gene or accession: CG16796 *F Gene annotation error *F Genes CG4311 and CG167796 should be merged. *F Comments: I've been looking at HMG-CoA synthase sequences. According to *F GeneScene, CG4311 and CG16796 represent alternatively spliced forms of the *F same locus. Not sure if they have a common start transcription site, but they *F splice to a common exon which encodes the HMG-S ORF. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 98; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128937 *a Fasano *b L. *t 2000.4.11 *T personal communication to FlyBase *u FlyBase error report for CG12630 on Tue Apr 11 03:27:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 11 11:23:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Apr 2000 11:23:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 11 Apr 2000 03:27:53 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 314 *F Error report from Fasano laurent (fasano@lgpd.univ-mrs.fr) *F Gene or accession: CG12630 *F cDNA or EST error *F Comments: Sequence of a cDNA shows that the first exon is missing. Check the *F GenBank file AF219383. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128938 *a Greene *b E.A. *t 2000.4.11 *T personal communication to FlyBase *u FlyBase error report for CG15844 on Tue Apr 11 15:26:15 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 11 23:21:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Apr 2000 23:21:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 11 Apr 2000 15:26:15 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 305 *F Error report from E A Greene (eagreene@fhcrc.org) *F Gene or accession: CG15844 *F Gene annotation error *F Gene CG15844 corresponds to FBgn0004377 *F Comments: The link from the gene name to the FlyBase symbol is missing *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128939 *a Greene *b E.A. *t 2000.4.11 *T personal communication to FlyBase *u FlyBase error report for CG6392 on Tue Apr 15:28:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 11 23:24:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Apr 2000 23:24:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 11 Apr 2000 15:28:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 310 *F Error report from E A Greene (eagreene@fhcrc.org) *F Gene or accession: CG6392 *F Gene annotation error *F Gene CG6392 corresponds to FBgn0040232 *F Comments: The links between the gene name and the FlyBase symbol are missing. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128940 *a Greene *b E.A. *t 2000.4.11 *T personal communication to FlyBase *u FlyBase error report for CG4831 on Tue Apr 11 15:31:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 11 23:26:44 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Apr 2000 23:26:44 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 11 Apr 2000 15:31:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 310 *F Error report from E A Greene (eagreene@fhcrc.org) *F Gene or accession: CG4831 *F Gene annotation error *F Gene CG4831 corresponds to FBgn0040233 *F Comments: The links between the gene name and the FlyBase symbol are missing. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128941 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG12840 on Thu Apr 13 09:21:30 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 17:16:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 17:16:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 09:21:30 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 709 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12840 *F Gene annotation error *F Gene see below has incorrect exon/intron structure. *F Comments: The annotated cDNA for this protein is correct, but for some reason *F the annotated translation does not employ the first start codon, thus removing *F the first TM domain of these tetraspanin proteins. The full translation *F should be *F MGCATGTIKYSLFLFNALWAILGILVLIFGGLGWGAMPDAYAIGILILGGTILVISLFGCCGAVRESPRMLWTYASLLLI *F LLLLIVAFIILNPKDVFKKYALQTVENQWELEQTKPGSMDIIQKTYYCCGRDSAQDYLDIKFWNNTVPSSCCKDDSCVNP *F LNLYVRGCLIKVEEAFADEATTLGYLEWGLLGFNAVILLLAIILAIHYTNRRRRYNY *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128942 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG8666 on Thu Apr 13 10:36:03 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 18:31:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 18:31:27 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 10:36:03 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1451 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG8666 *F Gene annotation error *F Gene CG8666 has incorrect exon/intron structure. *F Comments: By homology arguments, we identify an additional 5' exon for this *F gene that encodes the first TM domain of these tetraspanins, and also modify *F your 3' exon to better encode the C-terminus. *F Our proposed cDNA sequence is *F ATGGCATCCGGCGGCTTAACATGTGTAAAATACTTGACATTCTTTTGCAATCTTCTTTTTGCGCTCACTGGGCTTCTTAT *F TTTTCTGGTGGGAGGCATGGTCCAACTAAACTATGCACATTATTCGAACTTCGTTAGCGATCACGTCTGGACCGCTCCTA *F TCATACTTATGATTGTCGGTGCCGCTGTGGCAGTCATTTGCTTCTTGGGATGTTGTGGAGCCTTAAAAGAGAGCAGCTGT *F ATGATACTTAGCTTTGCCCTTCTTGCCGTCGTGATATTTTTGTTCGAGATCGGACTGGGCCTTGCTGGCTATGTGAAGCA *F TACCGGTCTTCATCAGATCATGGAGAGTCAGTTTAACTCGACCATGCAGCACTACAAGGAACGGGCCGACTATCGCGATG *F CTTGGACTTTGCTGCAAACCGAGCTAGATTGTTGCGGCATCAATGGGCCCAACGACTGGGAAACCGTTTACCGTAATAGT *F ACCCTACCTGCAGCCTGCTGCTCTGTAATTAATTTAAGCGAGGCCAAGGAATGTACAAATACTCACGCAACTCAGCATGG *F CTGCTTGCAGAAGCTCTTAGAGATTTTGGATTCAAAGACCTTAATTTTGGCCTCCGTTGTTTTGGGAGTGGCGGGTATAC *F AGATGCTCACTATACTGTTCGCTTGCTGCCTCTATCGTTCGTTTCGCAGGAGCTACGATCATGTTTAA *F Our translation is *F MASGGLTCVKYLTFFCNLLFALTGLLIFLVGGMVQLNYAHYSNFVSDHVWTAPIILMIVGAAVAVICFLGCCGALKESSC *F MILSFALLAVVIFLFEIGLGLAGYVKHTGLHQIMESQFNSTMQHYKERADYRDAWTLLQTELDCCGINGPNDWETVYRNS *F TLPAACCSVINLSEAKECTNTHATQHGCLQKLLEILDSKTLILASVVLGVAGIQMLTILFACCLYRSFRRSYDHV *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128943 *a Greene *b E.A. *t 2000.4.11 *T personal communication to FlyBase *u FlyBase error report for CG17461 on Tue Apr 11 15:38:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 11 23:33:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Apr 2000 23:33:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 11 Apr 2000 15:38:08 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 529 *F Error report from E A Greene (eagreene@fhcrc.org) *F Gene or accession: CG17461 *F Gene annotation error *F Genes CG17459 and CG17461 should be merged. *F Comments: A single kinesin motor domain would be formed by the translated *F product of the merger of the two adjacent predicted genes. Separately the *F predicted gene products could not function. Either these two together form *F one gene with a large intron, or we're looking at pseudogenes. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128944 *a Mechler *b B.M. *t 2000.4.12 *T personal communication to FlyBase *u FlyBase error report for CG5462 on Wed Apr 12 08:47:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 12 16:42:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Apr 2000 16:42:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 12 Apr 2000 08:47:00 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 3118 *F Error report from Bernard M. Mechler (dev.genetics@dkfz-heidelberg.de) *F Gene or accession: CG5462 *F Missed gene *F Comments: The CG5462 gene corresponds to the scibble gene described by Bilder *F and Perrimon and to the vartul gene that we have independently isolated. *F Bilder and Perrimon has identified a single transcript of 6.0 kb in size that *F they have submitted to GenBank/EMBL/NCBI whereas our studies showed that this *F gene encodes 3 transcripts of 7.2, 6.0 and 4.5 kb in size, respectively. The *F 7.2 (AC: AJ252084) and 6.0 transcripts differ by the length of their 3'UTR and *F are made of 23 exons distributed over 53-kb genomic DNA. The Celera genomic *F sequence is larger because it contains a Jocker repetitive element which is *F missing in the genomic DNA sequence that we have analyzed. The 4.6 kb *F transcript (AC: AJ271647) consists of 14 exons whose first 13 exons are *F identical with those of the largest 7.2 and 6.0 kb transcripts. The largest *F cDNAs contain an open reading frame of 5268 (without the termination codon) *F encoding a protein of 1756 amino acids in length with an estimated molecular *F mass! *F of 190.382. This protein is designated as Vartul1. The alternatively spliced *F 4.6 kb mRNA encodes the Vartul2 protein containing 1247 amino acids with an *F estimated molecular mass of 136.436. Both Vartul1 and Vartul2 share identical *F sequence over the first 1143 amino acids but differ by their C-extremity. Both *F proteins are recognised by affinity-purified antibodies raised against an *F N-terminal synthetic peptide and display a size of ~220K and ~140k on Western *F blots of Drosophila protein extracts. The N-terminal domain of both proteins *F contains 16 contiguous LRRs with a repeating 23-residue consensus sequence. *F Four PDZ domains are present in Vartul1 whereas only the first two PDZ domains *F are including in Vartul2. The highest sequence identity of Vartul1 is to the *F putative human protein KIAA0147 (AC: D63481). *F The following ESTs belongs to Vartul transcripts (7.2, 6.0, and 4.6-kb *F transcripts): LD28165,LD11253,LD43114,LD43989,LD15830,LD16092,LD13923, *F GH23755,and GH27616 (we have not sequenced these ESTs. GM12994, SD05339, *F LD37377, and CK00392 belong to the 7.2 and 6.0-kb class of transcripts (we *F have not sequences the 3' end of these ESTs). However we have sequenced *F completely LD26440, LD22510 and LD32992. These three ESTs belong to the 6.0-kb *F class of transcript with a short 3'UTR. The EST SD05630 3' sequence *F corresponds to the 3' end of the 7.2-kb transcript. The ESTs LD15830 and *F GM13360 belong to the 4.5-kb alternatively spliced transcript (we have *F completely sequences LD15830). *F For determining the exon-intron borders, I have aligned the three size-classes *F of transcripts with the Celera genomic sequences deposited in Dec. 1999 *F (AC013024 and AC012994) but have not yet made the alignment with the new *F contig. Theere are some sequence divergences reflecting the different *F libraries that we have used. Most of the divergences reflect silent *F substitution or lead to conservative amino acid substitution. *F Browser: Mozilla/4.05 de (WinNT; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128945 *a Mumford *b K. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG5067 on Thu Apr 13 04:31:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 12:26:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 12:26:47 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 04:31:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 460 *F Error report from KATIE MUMFORD (katie.mumford@port.ac.uk) *F Gene or accession: CG5067 *F Gene annotation error *F Gene CG5067 corresponds to aj252268 (FBgn) *F Comments: The accession number is for the EMBL database. The gene has *F published, *F its is called capicua and the paper ref. is : *F Jimenez, G.; Guichet, A.; Ephrussi,A. and Casanova, J. *F Genes and development 14:224-231 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128946 *a Whitfield *b E. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG9900 on Thu Apr 13 06:28:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 14:24:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 14:24:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 06:28:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 6331 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9900 *F Gene annotation error *F Genes CG990, FBgn0029627 and mit(1)15, FBgn0004643 should be merged. *F Comments: Sequence alignment of: *F 1) *F AE003424; AAF45794.1 *F (Celera full length 721aa sequence, strain y; cn bw sp) *F 2) *F X64390; CAB76122.1 *F (Williams et al, Medline: 92363920, full length 721aa sequence, strain *F unidentified) *F 3) *F AL138972; CAB72295.1 *F (EDGP full length 721aa sequence, strain Oregon-R) *F 4) *F AL121804; CAB65854.1 *F (EDGP C-terminal 461aa fragment, strain Oregon-R) *F demonstrates that CG9900 and mit(1)15 are in fact the same gene, clustal shown *F below. *F I was concerned that the map position of CG9900 is given as 3A4 and mit(1)15 *F as 3A9, but looking at the evidence for mit(1)15: *F from star coded flat file of genes *F \*c 3A9 *F \*c Left limit from inclusion within Df(1)z-deb81 (FBrf0049883) *F \*c Right limit from inclusion within Df(1)z-EM46 (FBrf0054120) *F and at the individual Df breakpoint positions, mit(1)15 is actually between *F 3A1 (Df(1)z-deb81) and 3A9 (Df(1)z-EM46), making 3A4 a possible position. *F I hope with this evidence you agree with me, *F Regards *F Eleanor Whitfield *F Swiss-Prot annotator *F EMBL-European Bioinformatics Institute Tel: \+44-(0)1223-494680 *F Wellcome Trust Genome Campus, Hinxton, Fax: \+44-(0)1223-494468 *F Cambridge CB10 1SD, UK e-mail: eleanor@ebi.ac.uk *F CLUSTAL W (1.8) multiple sequence alignment *F Celera sequence is represented by Swiss-prot accession number: Q9W4X9 *F CAB72295 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPN *F CAB65854 \------------------------------------------------------------ *F CAB76122 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFMPN *F Q9W4X9 MEEEAPRFNVLEEAFNGNGNGCANVEATQSAILKVLTRVNRFQMRVRKHIEDNYTEFLPN *F CAB72295 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV *F CAB65854 \------------------------------------------------------------ *F CAB76122 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV *F Q9W4X9 NTSPDIFLEESGSLNREIHDMLENLGSEGLDALDEANVKMAGNGRQLREILLGLGVSEHV *F CAB72295 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY *F CAB65854 \------------------------------------------------------------ *F CAB76122 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY *F Q9W4X9 LRIDELFQCVEEAKATKDYLVLLDLVGRLRAFIYGDDSVDGDAQVATPEVRRIFKALECY *F CAB72295 ETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE *F CAB65854 \------------------------------------------------------------ *F CAB76122 ETIKVKYHVQAYMLQQSLQERFARLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE *F Q9W4X9 ETIKVKYHVQAYMLQQSLQERFDRLVQLQCKSFPTSRCVTLQVSRDQTQLQDIVQALFQE *F CAB72295 PYNPARLAEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ *F CAB65854 \--------------------MRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ *F CAB76122 PYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAHVRPNYKQ *F Q9W4X9 PYNPARLCEFLLDNCIEPVIMRPVMADYSEEADGGTYVRLSLSYATKEPSSAQLRPNYKQ *F CAB72295 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS *F CAB65854 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS *F CAB76122 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS *F Q9W4X9 VLENLRLLLHTLAGINCSVSRDQHVFGIIGDHVKDKMLKLLVDECLIPAVPESTEEYQTS *F CAB72295 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD *F CAB65854 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD *F CAB76122 TLCEDAAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD *F Q9W4X9 TLCEDVAQLEQLLVDSFIINPEQDRALGQFVEKYETYYRNRMYRRVLETAREIIQRDLQD *F CAB72295 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS *F CAB65854 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS *F CAB76122 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS *F Q9W4X9 MVLVAPNNHSAEVANDPFLFPRCMISKSAQDFVKLMDRILRQPTDKLGDQEADPIAGVIS *F CAB72295 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT *F CAB65854 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT *F CAB76122 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT *F Q9W4X9 IMLHTYINEVPKVHRKLLESIPQQAVLFHNNCMFFTHWVAQHANKGIESLAALAKTLQAT *F CAB72295 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET *F CAB65854 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET *F CAB76122 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET *F Q9W4X9 GQQHFRVQVDYQSSILMGIMQEFEFESTHTLGSGPLKLVRQCLRQLELLKNVWANVLPET *F CAB72295 VYNATFCELINTFVAELIRRVFTLRHISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL *F CAB65854 VYNATFCELINTFVAELIRRVFTLRHISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL *F CAB76122 VYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL *F Q9W4X9 VYNATFCELINTFVAELIRRVFTLRDISAQMACELSDLIDVVLQRAPTLFREPNEVVQVL *F CAB72295 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI *F CAB65854 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI *F CAB76122 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI *F Q9W4X9 SWLKLQQLKAMLNASLMEITELWGDGVGPLTASYKSDEIKHLIRALFQDTDWRAKAITQI *F CAB72295 V *F CAB65854 V *F CAB76122 V *F Q9W4X9 V *F \* *F Differences between y; cn bw sp and Oregon-R (AL138972; CAB72295 and AL121804; *F CAB65854) *F 248 248 C \-> A *F 293 294 QL \-> HV *F 626 626 D \-> H *F Differences between y; cn bw sp and Williams et al (X64390; CAB76122) *F 58 58 L \-> M *F 203 203 D \-> A *F 293 294 QL \-> HV *F 366 366 V \-> A *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128947 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 13 09:01:06 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 16:56:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 16:56:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 09:01:06 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1843 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: I believe there is an unannotated gene encoding a tetraspanin (four *F transmembrane protein) in gb|AE003842.1. *F We have sequenced a BDGP cDNA encoding this protein, called GH05668, and it *F has also been submitted to GenBank by Rubin's group. The cDNA sequence is *F TTAGCATTGTCAACTGCTCACGAACGGTTCGAAAAGCGGAGCGCGCGTAAAATCATTCTGTAAATCATTCAAAAGGCGGA *F AAACTCAAGTTGCAGTTCTTCATCCATCTCCACCAGCAATCTCTGGCAAAACTCAGGCAAAATGAGCTGCGGAATCTCCA *F TGGTTAAATATATCCTATTTATATTTAATTTGCTCTGTTCGATATGCGGCATATTGCTGATCGTATTCGGAGCTCTGCTG *F TTCAGCAAAGTCCGTAACATGGATGACTTCGCGGAAGCCCTGCGAACCCAGCAGGTGCCCGTAACGATGATCATCCTGGG *F CACCATCATCCTGCTGATTTCCTGGTTCGGCTGCTGCGGAGCCATTCGGGAATCCTACTGCATGTCCATGACGTACTCGA *F TCTTGCTGATCGACCTGATGATTGGCCAACTGGCTTTGGTGATCTACATGTGGGTGCAGAAGGACAAGTACCTGGAGATC *F ATGGGCGACGTGGTCGAGAAGGCCTGGAACCATCGCACCAGTCGTTCCGACTACATGGACGCGATTCAGATCAGCATGAA *F ATGCTGCGGACGCAGTGGCTACACCGACTACGCCTACCAGGGCAAGTTCCCTCCCTCCTGCTGCAGCGACACCAACAACT *F GCCGCTGGGAGACCGTCTACCGGCGGGGATGCAAGGTCACCTTCGTTGAGTTCTGGGACAGGAACAGCGACATCATCAAG *F TATGCCGGTCTGGTCATCGCCGCCATCGAATTTGTGGGATTCGTTTTCGCCTGTTGCTTGGCGAACAGCATTCGGAACTA *F TAGACGCCGTGCGGAATATTAATCGACAAAGGACTAAGGCCTTGCACTAATTTTAATTGAAACCGAAAGTACGAATTATG *F TTGCCCAATTTTACGAATATTTACCTGATACAGATGGCCATTCAAATTTGCATAATCTCAAGCGTAAGCAGCAAATGCAG *F CAAATCCAATGACGAATGCGTAACGATCACTTTTGTAAGATCGTTTGTTCAAAGTTACACTGAATGTGCTAATATGTTTA *F ACTGTACAAAATAACTTATACTCCTGGAGATTGCAATAAACGGAGAAATTTATTTACAATTTAAAAAAAAAAAA *F The encoded protein is *F MSCGISMVKYILFIFNLLCSICGILLIVFGALLFSKVRNMDDFAEALRTQQVPVTMIILGTIILLISWFGCCGAIRESYC *F MSMTYSILLIDLMIGQLALVIYMWVQKDKYLEIMGDVVEKAWNHRTSRSDYMDAIQISMKCCGRSGYTDYAYQGKFPPSC *F CSDTNNCRWETVYRRGCKVTFVEFWDRNSDIIKYAGLVIAAIEFVGFVFACCLANSIRNYRRRAEY *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128948 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 13 09:15:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 17:11:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 17:11:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 09:15:53 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 2036 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: I believe there is an unannotated gene encoding a tetraspanin (four *F transmembrane protein) in gb|AE003842.1 and gb|AE003790.1. *F We have sequenced a BDGP cDNA, LP06288, with the sequence *F TCACATCGAACGAACGGATTATCCAGCTAGCCAACGAGAAACCCGAACAGAACCCAGCACCATGAACTGTCTATCCGCGA *F TGTTCAAGTACCTGCTGTACTTGCTCAACCTGGTGTTCGTGGCCGGTGGCATCCTGCTCATTGTGGTGGGCTCCATCATG *F CTCTCCACGATGGGCAACTTTACGGCCTTCGACGGAGGCGTTAACACCCAGACCATCCCGATCTGCATTATCGTCATCGG *F AAGTGTCACCTTCGTAGTGGCCTTCTTCGGATGCTGCGGCACCATTCGCGAGAACGCCTGCTGCACCACCATCTACGCCA *F TCTGCATGCTGATTCTGTTCGGCCTGCAACTGGCCCTCTCCATCTGGATCTTCGCGGCCAACGACAAGTTCCTGTCCAGC *F ATGGGCAAGGCAGTGGACAAGGCGTGGGATGAGAACAATGCCGCCCAGGGATACCCCATGGATGCCCTCCAGTTGGCCTT *F CTCTTGCTGTGGCAACACGGGATACCAACAGTATGAAACCGTGCCCAGCTCCTGCTGCGGCTACAAGGATCGCACCAAGG *F TGTGCGAAGCGGAGATCTACAGCCAGCGACCTGGCTGCCGGCAGGAGTTCGTCGATTTCTGGGCCTCCAATACGGACCTG *F ATTCGATGGAGCAGTCTGATCATCGCCCTCTTCGAGCTGGGCATCTTCATCATGTCGTGCTGCCTGGCCAGCGCGATGAG *F GAAGCGCTAGAGCCGAAGTCAGATACAATCCTTAGACGTAGCCCAGTTCATAGCATAAATCCAACACACGAATTCCTCAC *F TCACTCACTCACCGTAAATAAACCAGAATAGGTTAAGCCCAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAA *F It encodes a tetraspanin with sequence *F MNCLSAMFKYLLYLLNLVFVAGGILLIVVGSIMLSTMGNFTAFDGGVNTQTIPICIIVIGSVTFVVAFFGCCGTIRENAC *F CTTIYAICMLILFGLQLALSIWIFAANDKFLSSMGKAVDKAWDENNAAQGYPMDALQLAFSCCGNTGYQQYETVPSSCCG *F YKDRTKVCEAEIYSQRPGCRQEFVDFWASNTDLIRWSSLIIALFELGIFIMSCCLASAMRKR *F There seem to be problems with the Celera sequences here. They have two *F scaffolds that have this sequence, which would seem to imply that they overlap *F and therefore should be one scaffold. Secondly, the sequence in each of these *F two scaffolds seems to have a single base insertion causing a frameshift, *F immediately after the following twenty base pairs (CTGCAACTGGCCCTCTCCAT). *F This single base insert is also not present in the htgs sequence of *F gb|AC008288.7 and gb|AC009342.4 from the Rubin lab. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128949 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG12847 on Thu Apr 13 09:46:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 17:42:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 17:42:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 09:46:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1990 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12847 *F Gene annotation error *F Gene CG12847 *F MTGAYTSMVFVLFILQLVLTCWVFVNRSAFLGDMSNLVNLLWDSHDYTAMGVLEETFGCCGDTSYTNYNNIGLSVPGTC *F C GYLDRQATCNTPSVYQSRPGCSAKFEEFWNDNMDIIRWSGLGLCIFDLVVFLIAGALTNCMRSQNAGRQVYA *F has incorrect exon/intron structure. *F Comments: We have sequenced a BDGP cDNA, LP02831, encoding this tetraspanin *F (four transmembrane protein), and it shows an additional 5' exon separated by *F an intron of about 1100bp. This exon encodes the first of the four TM domains *F of these proteins. *F The cDNA sequence is *F TCCAATCGAACGACAGTAAACGACGCGAGTGCGCGATAAAATCAGAGACACCGACAACTCCTTTGGATAAAACAATAGTG *F TTTATTTAATTCTACTAAATACAGGAGCAAACTATATACTTTCAAAATGGGTTGTCTATCGGGAATAGTCAACTTTATTT *F TATATATTGTCAATATCGTGTTTTTGATCGTTGGCATCCTACTGATCGTGTTGGGCTCGATCATGCTGTCCGATCTGAGC *F CGCTTCGATGTCGCGGGGAGTGGGACGGACCCGAACACCATCCCCATCTGCGTCACCGTCCTGGGAGGCCTCATCTTCGT *F GGTGTCCTTCTTCGGGTGCTACGGCATTTTTCGGCAGAGTGTCTGCATGACCGGCGCGTACACCAGCATGGTTTTTGTGC *F TCTTTATCCTGCAACTGGTGCTTACGTGCTGGGTGTTCGTGAACCGATCTGCCTTCCTGGGCGATATGTCCAATCTGGTT *F AACTTGCTCTGGGACTCCCATGACTACACTGCCATGGGCGTTCTTGAGGAAACCTTCGGCTGCTGCGGTGATACGAGCTA *F TACCAACTACAACAACATCGGCCTTTCGGTTCCCGGAACCTGCTGCGGCTACCTGGACCGCCAGGCCACATGCAACACCC *F CCTCGGTCTACCAGTCGAGGCCCGGCTGCAGCGCCAAGTTCGAGGAGTTCTGGAACGACAACATGGACATCATCCGCTGG *F TCCGGCCTCGGCCTCTGCATCTTCGACCTGGTCGTCTTCCTCATCGCCGGCGCCCTGACCAACTGCATGCGCAGCCAGAA *F CGCAGGTCGCCAGGTGTACGCCTAAACTTGTGAGGAACAAGCCAAAGGCCAAAGGATCTACATATGTCTACTTATGTTAC *F CATATAACAAACTGTTTTTCGAGCCGTGCCAATATTAATATATACGTCTACATTTCGCCTATTTATCAGTTACTACTTAT *F GTTATTCTTTATACTCTTTTTGGAGCAATGCCAATATTAATATATACCGCTACAACATTTAAAAAAAAAAAAAAA *F and the encoded proteins is *F MGCLSGIVNFILYIVNIVFLIVGILLIVLGSIMLSDLSRFDVAGSGTDPNTIPICVTVLGGLIFVVSFFGCYGIFRQSVC *F MTGAYTSMVFVLFILQLVLTCWVFVNRSAFLGDMSNLVNLLWDSHDYTAMGVLEETFGCCGDTSYTNYNNIGLSVPGTCC *F GYLDRQATCNTPSVYQSRPGCSAKFEEFWNDNMDIIRWSGLGLCIFDLVVFLIAGALTNCMRSQNAGRQVYA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128950 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG12843 on Thu Apr 13 09:53:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 17:49:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 17:49:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 09:53:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1849 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12843 *F Gene annotation error *F Gene CG12843 has incorrect exon/intron structure. *F Comments: We have sequenced a BDGP cDNA, LP02708, which does not show the last *F intron/exon structure you annotate, and hence changes the C-terminus of this *F tetraspanin. *F Our cDNA sequence is *F AGCTGAACCACGGCGGTCGGACGTCGAAAGATCTTAGCGCGCCGCTGTATCCGAAAGATACGCATCTGAAACAGGCTGCT *F GGCCATATAGCCTTATATGTAAGGCCTCTGATGCACTCTACTGCGCGTTGCCTTCGTACATCTTAACAGTCGGTCTCCAG *F CCGCATCTACAGGTTGATTGATCCACAGTAATCATGGGTCTGGGCGCCACTACAGTGAAGCATGTGCTGCTTTTGCTGAA *F CTTTGTGTTTTCCGTGCTCGGGCTGGCGCTGATCGCCTTCGGAATTTTCTTTTTGATCTCCGCCGCTGAGAATGCAGTCA *F GCATTGGGAAAAATGCGGCCGGGGGCCTAATCATCGCCTTGGGCGTTGTCATCCTCATCATTGCGATCTTCGGCTGCCTG *F GCTGCCATCCATGAGGCTCCTGTGAGGCTCCTCATATATGTGGGAGCCGTGGTGCTGCTGATCCTCGCCCAGCTAATTTT *F TCTCGGCATGTCCTCACACGGCACCAAGGATGGAATCTCGGGCAGCATCAACGAGGGATTCGACCGTCTCTGGGAATCGG *F AGCGCAACCAAACAGGCGCCTTGAGCTACTACGAGTCCTGGCTGCAGTGCTGCGGGGTCAACAGTTCCGAGGACTACTGG *F ATCATCCATCATGGCATTCCGTCCAGCTGTTGTCCAGAAAGCAAGTGCATGGACACTCCGAGCAGGGTCTTCAAGACGGG *F CTGCAAGGCCGCATTCGTGAAGTATCTGGACGATAAGTTACTGGTGTTCAAAATCGTCTGCTGGTTGCTTGTCATCGGAG *F AGGCTGTGGGAGCTGTTTTCGGTTGGCTGCTCTACAGCAGCGTAAAGAACCAAAGCCGCCGCAACAATGCCGTCTGGATG *F TGAGGGGTTTTCTAAGTCATCTTAGTATTAATTAACAATATATTTGAATCTGTTTTCTAATCAAATGTTTCCTACTTAAA *F TATTTAATAACCGATTACTAAAGGCAAACCCAATCTGTACAAAGGGCTTAAGATTTTAGGTACGATTTTCGAGAAATTTG *F TAATGGGAATAATGTTCATATATTGATAACGTCTTTTTGATAACGATAGTCACATAAAGTTATTACTCTTTAACCAAAAT *F CTAAAAAAAAAAAAAAAAAAA *F So our translation is *F MGLGATTVKHVLLLLNFVFSVLGLALIAFGIFFLISAAENAVSIGKNAAGGLIIALGVVILIIAIFGCLAAIHEAPVRLL *F IYVGAVVLLILAQLIFLGMSSHGTKDGISGSINEGFDRLWESERNQTGALSYYESWLQCCGVNSSEDYWIIHHGIPSSCC *F PESKCMDTPSRVFKTGCKAAFVKYLDDKLLVFKIVCWLLVIGEAVGAVFGWLLYSSVKNQSRRNNAVWM *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128951 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG12846 on Thu Apr 13 09:57:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 17:53:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 17:53:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 09:57:43 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1560 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12846 *F Gene annotation error *F Gene CG12846 has incorrect exon/intron structure. *F Comments: We have sequenced a BDGP cDNA, GH07074, that has an additional 5' *F exon which encodes the appropriate TM1 domain of these tetraspanins. *F Our cDNA sequence is *F GACCACGAACGAAATCGCATCGAACGTCTTACTCTTAAATCGAACACAAGTGATACACAAGTACATCTCAGCAATGGATT *F GCGGCGGCGTTTTTGTGAAATATGTGCTGTTCATATTCAACATACTGTTTGTGATATGCGGCATTTTGCTTATCACCTTC *F GGCTCCATCATGGTGTCCACCATAAAGGACTTCTCGGGCGTTGGCGAGACCTTCACGGCAAACAGCGTGGCCATCATCAT *F CCTGGTCCTTGGCTGCGTAGTCTTCCTGGTAGCCTTCATGGGATGCTGCGGCGCCATACGCGAGAATTCCTGTGCTCTGA *F CCTCGTACTCTGTGGTCATGCTGGTGCTGTTGGTTAGTCAGCTAGCTCTCATTATCTACGTGTGGGTGGACCATGTGCAG *F ATACAGCAATCTTTGGAGAAGATCGTCCAGACCATATGGGATCAACGCAAGACCGATGCCCTCCTCATGGACACACTGCA *F GCGATCGTTCAAGTGCTGCGGCTTGAACGGCTTCGCTGATTACGGCATTACGTATCCCGCCTCCTGCTGCGACTCGCCCT *F CCAATGGAACCTGCGCACTAACCCAAGTCATGACGCGATCCAGTTGCCTGAAGGCCGTTGATTCCTTCTGGGACACCAAC *F GTGAGCATCATCAAGTACGCTGGCCTGGGTGTGACTGCTGTTGAGCTTGTGGCCTTCATTTTCGCCTGCTGCCTGGCCAA *F TCAGACCCGCAACTCGCAGAGACGCCAGAACTACTAAACGATGAAACGATGAATTAGTAGCATAGTATTAGGATTATCTC *F CCGACCCTCTTTAACGTCATAAAGTCAATATGAATAAATAAAACAAAGTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAA *F And our translation is *F MDCGGVFVKYVLFIFNILFVICGILLITFGSIMVSTIKDFSGVGETFTANSVAIIILVLGCVVFLVAFMGCCGAIRENSC *F ALTSYSVVMLVLLVSQLALIIYVWVDHVQIQQSLEKIVQTIWDQRKTDALLMDTLQRSFKCCGLNGFADYGITYPASCCD *F SPSNGTCALTQVMTRSSCLKAVDSFWDTNVSIIKYAGLGVTAVELVAFIFACCLANQTRNSQRRQNY *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128952 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG10742 on Thu Apr 13 10:05:24 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 18:00:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 18:00:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 10:05:24 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 971 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG10742 *F Gene annotation error *F Gene CG10742 has incorrect exon/intron structure. *F Comments: We have sequenced a BDGP cDNA, GH15125, which agrees with your *F annotated cDNA, however this is a strange cDNA because there are two start *F codons in other frames before the ORF. Furthermore, we propose that instead *F of starting translation with the first start codon of the ORF, you employ the *F third start codon. This provides a N-terminus that aligns in sequence and *F length much better with the other tetraspanins. Our translation is *F MHPHHFTYVSQCVKYMIFLLNFVFWLFGGLLLGIGVYAFRDKWEDANGSVRLENFYDVFLNISLVMILAGTDIFLVSFSG *F CVGALRENTFLLKFYSMCLLLFFLLEMAIAIVCFVCPQYMNTFLEKQFTHKIIHSYRDDPDLQNFIDFAQQEFKCCGLSN *F SGYQDWSKNEYFNCSSPSVEKCGVPYSCCINATDISSGLVNIMCGYGVQNAPVPEATKLIWTSGCIEIVRVWAEHNLYVI *F AGNALGIALIQLLVIYLAKTLEGQIELQKSRWLA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128953 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG9494 on Thu Apr 13 10:30:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 18:25:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 18:25:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 10:30:31 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1768 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG9494 *F Gene annotation error *F Gene CG9494 has incorrect exon/intron structure. *F Comments: We have sequenced a BDGP cDNA, LP06047, from this gene, and while it *F agrees with your annotation for the 5' end, it differs for the 3' end. Alos, *F we start the translation earlier, thus encoding the needed TM1 and TM2 domains *F of these tetraspanins. *F Our cDNA sequence is *F TTAAACTAAAATTGTCCACTTTCTGTGTGCCCGCATAAACTAAAACAAACAAGATGTCGCTTCTAACGGGCAGCGCCAAT *F GCTGTGAAGTATACGCTTTTCGGATTTAACTTAATTTTTTTGATCACTGGCATTATCTTGATTGCCGTGGGAGCCGGAGT *F TGGCGCCGTCTATACGGGCTATAAGCTCTTCCTGGCCGGAAAGTTCTTCTCGATCCCCACGTTCCTGATCGTGATTGGAT *F CGTTCATCATCATAATCTCTTTCTTTGGTTGCTGGGGTGCCCTGAAGGAGAACTATTGCCTGGTGCTCAGCTTCTCGGTC *F ATGCTGGCCATCATCTTCATCCTGGAGCTGGCTGCTGGCATCAGTGGCTATGTGCTGCGCAATGACGCCTCCGATTTGAT *F CAAAACTTCTCTGACTTACTCGCTGAACGAGTATAACAGTATCAATCCAAATGCGACCACGAAACTCTGGGATGACATCC *F AGGATGAGTTCGAGTGCTGTGGTGTGACCTCATACAACGACTGGATCACCGCCTTCCCTAACGGCGACCTGCCCATCTCC *F TGCTGCAACGTTCATGTCGGCGCAGTGGGCACATTCACCTGCAATAATGCTCAGTCCAGCGTGGCAGACCGGCACAAAGT *F TGGATGCCTCGACGGATTCTCCGGATACATTTCCGCCCATGCGGTCAGCCTAGGAGCTGCAGGGGTGGTCATTGCCATCC *F TCCAGTTCTTTGGCGTAATCTTTGCCTGCTACATTGCACGTGAGATTAAAATCCGTAATGGAATTACTGGTTTTATGTAG *F GTCCGGAGGAAATCGATTCTGTAGGATGAGATGAGCTAGATAAGAATGGTGCCGATATTTACTTTAAATTCAATGATGTT *F CTTGGACATATACTGACATTTACCCTTGCTTGTAAAAATAAATAAATATATTTCTAAACAAAAAAAAAAAAAAAAAA *F Our translation is *F MSLLTGSANAVKYTLFGFNLIFLITGIILIAVGAGVGAVYTGYKLFLAGKFFSIPTFLIVIGSFIIIISFFGCWGALKEN *F YCLVLSFSVMLAIIFILELAAGISGYVLRNDASDLIKTSLTYSLNEYNSINPNATTKLWDDIQDEFECCGVTSYNDWITA *F FPNGDLPISCCNVHVGAVGTFTCNNAQSSVADRHKVGCLDGFSGYISAHAVSLGAAGVVIAILQFFGVIFACYIAREIKI *F RNGITGFM *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128954 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG9033 on Thu Apr 13 10:47:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 18:42:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 18:42:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 10:47:08 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1458 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG9033 *F Gene annotation error *F Gene CG9033 has incorrect exon/intron structure. *F Comments: We propose a different 5' exon for this gene, which encodes a *F N-terminus for the protein that aligns better with other tetraspanins and also *F completes the first TM domain typical of these proteins. *F Our cDNA is *F ATGCGTTCTTGCGGTCCGAGTTTAATTAAATATGTCTTATTCGCATTTAATGTGCTGTTTGCGATCTCCGGCCTGGGAAT *F TCTTATTGCTGGTGCCGTTGTCCTGGCCGATGTGAATGAATTCAATCACTTTGTCGAAGGTCGAGTCCTTGCACCGCCAA *F TTGTCCTCATAGTCACGGGCCTGATCATATTCCTGATAGCTTCGCTGGGCTGTTTCGGAGCGATCAAGGAGTCTCCAACG *F CTTCTAATTACTTTTGCTGTCCTTTTGGCCGTCATCTTCATTGTGGAGCTGGCCGTCGGTATTGCGGCCAGCGTTTTCAA *F GAAGGATTTGGAGGGAATGGTGAAGAACTCGCTGCAGGAGTCCATCAAGCGATCGAACAGCGAAGACACCATGGCATGGG *F ACAACATCCAGCAGAAGTTGATGTGCTGCGGAGTCGACTCTCCGGCGGACTGGAGGACACTCAGTGCGAACAAAACGCTG *F CCGGGCAGCTGTTGTCAGCCGCAGTACATCGACTCGACCGTGGGTCACTGCCTGGAGTCACCGGCTCTCGGCAAGGATAA *F GTACTTCCAGGTCGGCTGTGTTGGCAAGCTGAAGGATCGAATCGAGAAGAACGCCATCATCCTGATCGGTGTGGGCATCG *F GCATTGCTTTTATCCAGATTTTGGGCATCGTTCTGGCCTGCTATCTGGCCAACTCCATTCGACAGGAGCGGGCCAAGTAA *F Our translation is *F MRSCGPSLIKYVLFAFNVLFAISGLGILIAGAVVLADVNEFNHFVEGRVLAPPIVLIVTGLIIFLIASLGCFGAIKESPT *F LLITFAVLLAVIFIVELAVGIAASVFKKDLEGMVKNSLQESIKRSNSEDTMAWDNIQQKLMCCGVDSPADWRTLSANKTL *F PGSCCQPQYIDSTVGHCLESPALGKDKYFQVGCVGKLKDRIEKNAIILIGVGIGIAFIQILGIVLACYLANSIRQERAK *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128955 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 13 10:53:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 13 18:49:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 18:49:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 10:53:50 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1793 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: In gb|AE003536.1 I propose a new gene for a tetraspanin (four *F transmembrane protein). The cDNa is *F ATGGCCTGCTGTTTTAATTACAAATTTGTGCTCAATCTATGTAACTTTTTGTTTTTGATATGTGGCTTGCTGCTGGTAGT *F ATCAGGTCTATATATCTTCTCCGATAACAAACGCATTCTGCTCTCTAGACTTTTGGCTGCATCCAGCGATCGGCTGAGCT *F CACTTCCGCAGCCGCTGCTTTTTTATATAGCACTGGGTGTGGCGATTGCGGGATTTGTGGCTACTCTGGCTGCCGTTGTG *F GGTTTCTGGGCCAGTTGCCTTCACACCTATTGCTTTCTAACCATATACTTCCTGAGCGTTGTGGTGCTCCTGCTGACCGA *F ATCGGTTTTGTGCCTGGCCATCACATTGTGGCCACATTGCCTGGGAATCAGCTTAGATGAGACTCAGATGGTGCGATCGT *F TGCAGAGCAACTATGGAGTTCCGGGGCAGGAGCAGTTTACCAATGCCTTGGATTTGGCACAGGTCCGGTTCGGATGTTGT *F GGGATGAGAAGTTCCTTGGACTACGACACATCATTGTGGAGATTACAGGGCTATGGACAAAGGAATTGGCCGGTGCCGCT *F AAGTTGCTGTTTTCTTAAGAATGCCGGACATTCCATGGCATATCTGGACCCCAAGCCGGCCAATGAATCGATGTGTCAGT *F CTCTGGAACGATTGTCCTACGAAAGGGAACGCCACACGGAGTCATGTCTGCCCCATCTGGACAACTGGTATCGCGAACAG *F TACAGCATCTTTCTTGGCGCCAGTCTAATATTGGCCATGATTGAATTTTGTGTCCTACTCGCCATTATCATGAGCTGCAC *F CGGACTTGCTTCGCAACGGGCTAGGCTTAAGAAACCTGTTCAGGAAATGCGGACTCAAAAAGTAAAATCGCGACAAACCC *F TAATCGAGAATATATACGAACCTGATGTTGAACTGAGAGAAAATTCCAATCACAGTGGAGACGGAATATATCTGGGACCC *F GCTAGCAGACATGTCAGCAGTGAGGATTTCAAGGAGTTGTATATAAAACCGAGAGACTTGTACAAACAACATAATTTAAG *F AACAAGCCCAGCCAACCGACCAACACAAATGAGGAATTATTTGGTCTAA *F The encoded protein is *F MACCFNYKFVLNLCNFLFLICGLLLVVSGLYIFSDNKRILLSRLLAASSDRLSSLPQPLLFYIALGVAIAGFVATLAAVV *F GFWASCLHTYCFLTIYFLSVVVLLLTESVLCLAITLWPHCLGISLDETQMVRSLQSNYGVPGQEQFTNALDLAQVRFGCC *F GMRSSLDYDTSLWRLQGYGQRNWPVPLSCCFLKNAGHSMAYLDPKPANESMCQSLERLSYERERHTESCLPHLDNWYREQ *F YSIFLGASLILAMIEFCVLLAIIMSCTGLASQRARLKKPVQEMRTQKVKSRQTLIENIYEPDVELRENSNHSGDGIYLGP *F ASRHVSSEDFKELYIKPRDLYKQHNLRTSPANRPTQMRNYLV *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128956 *a Gelbart *b W. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report for CG18290 on Thu Apr 13 16:56:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 00:51:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 00:51:52 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 16:56:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 517 *F Error report from William Gelbart (gelbart@morgan.harvard.edu) *F Gene or accession: CG18290 *F DNA error at position 54796. Upstream nucleotides: gtattgtcctcgactccggg *F Insertion of length 1: g. *F Corrected sequence: gtattgtcctcgactccgg. *F Comments: CG18290 is identical to the first 157 aa of Act87E (accession *F CAA30982.1). *F With the frameshift as indicated, the remaining 219 aa of Act87E are also *F identical to CG18290. *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128957 *a Robertson *b H. *t 2000.4.13 *T personal communication to FlyBase *u FlyBase error report on Thu Apr 13 23:55:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 07:50:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 07:50:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Apr 2000 23:55:08 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1051 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: There is a seemingly fine new gene encoding a small secreted protein *F related to the A10 or OS-D protein in a small family we call SAPs (sensory *F appendage proteins) of unknown function (the other two previously unknown *F members are well annotated, CG9358 and CG11390, although the latter should use *F the methionine start codon two codons upstream) in gb|AE003462.1. *F My cDNA is *F ATGCTACTTTTAAATAAAAACCGAGTGATTTCTTTGGTCGTTAATTTTATTTTTCTTATCATTCTCATTTCATCGAGTGT *F CCAAGCGGATGAGAGAAACATTAACAAGCTGCTGAACAACCAGGTGGTGGTCAGCCGCCAGATTATGTGCATCCTGGGCA *F AGAGCGAATGCGACCAACTGGGACTTCAACTCAAAGCTGCCCTGCCAGAAGTTATAACCAGGAAATGCAGGAACTGCTCC *F CCACAACAGGCTCAAAAAGCGCAAAAGCTGACCACATTTCTGCAAACTAGATACCCGGACGTATGGGCCATGCTTCTAAG *F GAAGTACGACAGTGCCTAA *F My translation is *F MLLLNKNRVISLVVNFIFLIILISSSVQADERNINKLLNNQVVVSRQIMCILGKSECDQLGLQLKAALPEVITRKCRNCS *F PQQAQKAQKLTTFLQTRYPDVWAMLLRKYDSA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128958 *a Lasko *b P. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG18078 on Fri Apr 14 06:32:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 14:28:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 14:28:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 06:32:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 532 *F Error report from Paul Lasko (Paul_Lasko@maclan.mcgill.ca) *F Gene or accession: CG18078 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG18078. *F Comments: From BLAST searching this is clearly another quaking-related RNA *F binding protein, *F but the predicted peptide only includes the N-terminal 37 amino acids. I have *F not sorted out whether there is a sequencing error or an intron-exon assembly *F error. *F Browser: Mozilla/4.5 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128959 *a Roos *b C. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG18247 on Fri Apr 14 08:03:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 15:59:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 15:59:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 08:03:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 392 *F Error report from Christophe Roos (christophe.roos@helsinki.fi) *F Gene or accession: CG18247 *F Gene annotation error *F Gene CG18247 corresponds to U37773 (FBgn0015295) *F Comments: This is EMBL AC U37773 \- Gene shark *F Aka.: Drosophila melanogaster tyrosine kinase mRNA, complete cds. *F In Flybase as FBgn0015295 *F Browser: Mozilla/4.72 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128960 *a Roos *b C. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG10758 on Fri Apr 14 10:58:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 18:54:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 18:54:23 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 10:58:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 428 *F Error report from Christophe Roos (christophe.roos@helsinki.fi) *F Gene or accession: CG10758 *F Missed gene *F Comments: CG10758 shares exons with CG17348. *F While CG17348 is correctly predicted (gene drl, AC L47260) CG10758 seems so *F far to be an erroneous Genie prediction, untill it is proven to be a real *F alternate splice variant of derailed. *F Browser: Mozilla/4.72 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128961 *a Poole *b S. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG6246 on Fri Apr 14 11:35:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 19:31:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 19:31:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 11:35:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1185 *F Error report from Stephen Poole (poole@lifesci.ucsb.edu) *F Gene or accession: CG6246 *F Gene annotation error *F Gene CG6246 has incorrect exon/intron structure. *F Comments: The annotation in the genomic sequence for nubbin is way off, as is *F the predicted coding sequence and predicted protein. Using the coordinates of *F the sequence scaffold that contains nubbin that is returned at NCBI if you do *F a fly genomic blast with nubbin sequence, AE003636, the correct intron/exon *F structure should be: *F (coordinates from AE003636) *F exon 1: 220761-221102 AGTTT...AATCGATgtaa.... (exon in caps, intron *F lowercase) *F exon 2: 224952-224956 tttcagATATCA...GGATGgtaa... *F exon 3: 227432-229160 ttccagCTTCC...CAGCAGgtgt... *F exon 4: 229401-230110 cttgcagGTTC...TTAATATCAA *F Note: the first ATG in the nub ORF is at 220973; the start site of exon 1 was *F derived from the longest embryonic cDNA clone sequence and confirmed by primer *F extension and RNase protection. *F Ng, M., Diaz-Benjumea, F.J., Cohen, S.M reported alternative first exon and *F splicing in the wing disk, but gave no sequence data. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128962 *a Robertson *b H. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG12832 on Fri Apr 14 11:42:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 19:38:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 19:38:23 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 11:42:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1379 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12832 *F Gene annotation error *F Gene CG12832 has incorrect exon/intron structure. *F Comments: When compared with the other 36 genes encoding tetraspanins, this *F gene needs an additional intron near the C-terminus, which also provides a *F final exon encoding an appropriate TM4 region. *F Our proposed cDNA is *F ATGAGCTGCGGCACGAAGGCATTGAAGGTGTCCTCCTTCGTTTTGGACTTTCTGTGCTGTGTTCTGGCGGCACTGACCAT *F CGCGGCTTGCTCCTATGCCCTGATTGCTTTTAGCCACAGCGTAGCCATCCGGGTGCCCAGCATCCTGGGCATCGTCCTGG *F GCGGCCTGCTGTTCTTCAGCACGATCTTCGGCTGCATTGCTGCCCTGCGGGAGAGTATTCGCATGACCTGGATCTATGCC *F GCAATACTGCTGGCCCTGGTTTTCAGTCAGATCACCGTCATCTTGGCCCAACCCATCAACTATGAGCTGCTGGCCAACGA *F GACCATTTACGACGCCTGGCAGGGACAACTTTACCACTCGGACAGAATGTCCTACTTTGAGATCAAGTATCACTGCTGCG *F GACAGACGGGGCCCGCAAACTATCCGGATAGTGGCCTGGTGATTCCGCAAAGCTGCTACTTCAACCAGAACGCCACGGTG *F ACTACGGATCTCTATACGGTTGGGTGTAACCATCAGTTGGCAGCGGCCTTCGTCAAGGGAACTCGCTGGGAGAAGATCAC *F GGATTGGTCCGTTGTGGGTGTGGAGATACTCACTGTCATCATCGCCGGACTCCTGGCTATAACCTTACAGAATGCCGAGC *F GCCGCCGTCTTTACCGATAA *F Our translation is *F MSCGTKALKVSSFVLDFLCCVLAALTIAACSYALIAFSHSVAIRVPSILGIVLGGLLFFSTIFGCIAALRESIRMTWIYA *F AILLALVFSQITVILAQPINYELLANETIYDAWQGQLYHSDRMSYFEIKYHCCGQTGPANYPDSGLVIPQSCYFNQNATV *F TTDLYTVGCNHQLAAAFVKGTRWEKITDWSVVGVEILTVIIAGLLAITLQNAERRRLYRZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128963 *a Robertson *b H. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG12844 on Fri Apr 14 11:49:48 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 19:45:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 19:45:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 11:49:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1412 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12844 *F Gene annotation error *F Gene CG12844 has incorrect exon/intron structure. *F Comments: In order to obtain a translation that aligns better with other *F tetraspanins, including having the four TM domain, we propose an alternative *F 3' splice site for the third intron. *F Our cDNA is *F ATGTACTGCACCACCCGACTGCTCAGATACGTTCTGTGTGTCATCAGTGGCATCTGTGCCCTAGGTGGCTGCCTGCTCAT *F CTGGTACGGCGCCTGGCTGCTGGATAGCCTCTCTGAGGAGCAGCGGATGCTGGGAATGGATCACGGTGAGGACCTGGCCG *F CCGTTCTCTGCGTCCTGCTGGGCACCGTCATCGTGGTGGCCAGCATTTTCGGATCGGTGGCTGTGGCCAAGGACTCCAGG *F GTGCTGTTGATCTGCTACGCCGTTTTGTTGGTGTTTCTCCTGATCGTTCAGATTGTATTGGTCAGCATAAGCTATGCCGC *F CAGCCGAGATTTCCTACCGGATTCTTTGAGGCAGGGACTTGACGACCTGTGGGACTTGCAACACGAGGGCAACAGCACAC *F TGAACACCTACGAGGAGTGGCTTCACTGCTGTGGCCGCAACAGTGCCGAGGACTACCTGCACTTGGAGCAGATGCCTCCT *F CCCAGTTGTTGTCTCAATCGGGACTGCACCAAGCATCTGAATCTTTTCATGACAGGCTGCGAAGTCAAGTTCAAGGAGTA *F TGTCGGCGCAAAGACAGCCAACTTCCACTCGCTAAGTTGGTTCCTTGTTATCTTTGAGTTCGCTGGCTCGGTGACCACCT *F GCTACCTGGTAGACAGCATTCGCAATCATCGCGACCGCATAAGATTCTATAATTAA *F and our translation is *F MYCTTRLLRYVLCVISGICALGGCLLIWYGAWLLDSLSEEQRMLGMDHGEDLAAVLCVLLGTVIVVASIFGSVAVAKDSR *F VLLICYAVLLVFLLIVQIVLVSISYAASRDFLPDSLRQGLDDLWDLQHEGNSTLNTYEEWLHCCGRNSAEDYLHLEQMPP *F PSCCLNRDCTKHLNLFMTGCEVKFKEYVGAKTANFHSLSWFLVIFEFAGSVTTCYLVDSIRNHRDRIRFYNZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128964 *a Robertson *b H. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG12838 on Fri Apr 14 13:41:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 21:37:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 21:37:07 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 13:41:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1368 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12838 *F Gene annotation error *F Gene CG12838 has incorrect exon/intron structure. *F Comments: To align better with both the intron/exon structure, and to provide *F the appropriate fourth TM domain of these tetraspanins, I suggest an *F additional intron/exon at the 3' end of this gene. *F My cDNA is *F ATGCCCACGGTTCGCGTGTGCCTACAATGGACCTCGGTTGTCTTCAGTACGCTGACGTTGATCGTCGGCGTCCTTGCGGC *F CCTGGCCGGCGTCTATGAGCTGGATAAGTTCAACGAGGGCTCTGCGGAGCACACAGAGAAGTTCGTCCAACTGGGAATGG *F CAGGTGCTCTGATCTTGGCCGGACTGGTCGGTTGCCTGGGTGCCATATTCGGTTCCATCAAGGTGATGGTGGTGAACTTG *F ATTCTGCTTCTGGCTCTGATAGCCTCGCACATCTGGAAGGTGTCCCACTACAATGAGACCAAACAGCTGGACGCCACTGA *F GGTGTACGTGATGGATCTCTGGATGAAGGAGCTGGTCCATCACGGCGCCATGCAAGACTTGCAGCAGGAGTACGAGTGCT *F GTGGCGACAAGGGATTCTCCGACTACACGAGCCTCAACATGAAGGTGCCTCGCAGCTGTTTCCACACCAAGGACGGCATT *F CACGCCTTGTATCCGTATGGGGAGGGCTGCATGGCTGCCGTGAAGCGGGCTTATCTGCAGATCTACCGATACGAGAAATG *F GGTCCACTGCGGACTTATTGGCTACGAGGTCGTAGGCATCATCTTGGGTATCACCTTGTGCTGCCAGCTGACCAACAAGA *F CTCGTCGCTACACCTACTAA *F My translation is *F MPTVRVCLQWTSVVFSTLTLIVGVLAALAGVYELDKFNEGSAEHTEKFVQLGMAGALILAGLVGCLGAIFGSIKVMVVNL *F ILLLALIASHIWKVSHYNETKQLDATEVYVMDLWMKELVHHGAMQDLQQEYECCGDKGFSDYTSLNMKVPRSCFHTKDGI *F HALYPYGEGCMAAVKRAYLQIYRYEKWVHCGLIGYEVVGIILGITLCCQLTNKTRRYTYZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128965 *a Robertson *b H. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report for CG16991 on Fri Apr 14 15:30:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 14 23:25:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Apr 2000 23:25:24 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 15:30:02 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1494 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG16991 *F Gene annotation error *F Gene CG16991 has incorrect exon/intron structure. *F Comments: This is part of a tetraspanin, for which we think we have more *F approriate N and C-termini, although we are not fully confident of our *F N-terminal annotation. Ours does have the advantage of having several more of *F the expected TM domains, however the amino acids do not align well with *F conserved residues in this family. *F Our proposed cDNA is *F ATGCTTATTATAAGCCTATCCCTGTCCATGATCTTTGTTTATGTAGCCATCGTCGTGGTGAAACTGATAATTCTGTTCGA *F ACACATGCCTTGCTGGGATTGTTTGTTCAGGTCCTACATGATAATCGCTCTAACCGTAAATGCCCTAATGGCGCCGCTGC *F TGATAGTCGGTTTTTTCTTCATATATTCGCATTTATGCCGCGAAATACGCATTGTAATTTATGCCACAGTACTCTTCCTG *F GCAACATGGTTGCAGATGATGCTGACTATACTGTTTGCACAACAGTACCAAATTGTTGGAGATGTACTTCGAATATGGAT *F GAATCGCAAGAGTTTGGAATTCTACGAAAGTCGCTGCCAGTGCTGCGGAGTTTTGGGACCTGACGACTACAAGTTGGGCG *F ATCTGAATATCCCAAAATCCTGTTACAAGAATGGCAGTGAAAGGGATGAGGACCTGTACCGATCAGGCTGCTCCACGCGC *F TCCATAAAGCCCGCGTCGCCCATCATCCATGTGATCTCCTTCGTAATTCAGTATGTTTTAGTCATATGCATTGAGGTATT *F CCTTATTATCCTGTTAAGGAGCAAATCCCAACCCACAAGTATGTGGTCTGAGCGGGTCACCGAAAGGTTTGGTAGTGTAA *F AAAAGTGA *F Our translation is *F MLIISLSLSMIFVYVAIVVVKLIILFEHMPCWDCLFRSYMIIALTVNALMAPLLIVGFFFIYSHLCREIRIVIYATVLFL *F ATWLQMMLTILFAQQYQIVGDVLRIWMNRKSLEFYESRCQCCGVLGPDDYKLGDLNIPKSCYKNGSERDEDLYRSGCSTR *F SIKPASPIIHVISFVIQYVLVICIEVFLIILLRSKSQPTSMWSERVTERFGSVKK *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128966 *a Robertson *b H. *t 2000.4.14 *T personal communication to FlyBase *u FlyBase error report on Fri Apr 14 22:48:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 15 06:43:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 15 Apr 2000 06:43:52 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Apr 2000 22:48:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1448 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: In gb|AE003782.1 there is a highly divergent member of the *F tetraspanin (four transmembrane protein) family. It has all four conserved *F introns of this family, and the protein has all four typical TM domains, with *F four conserved cysteines in a large extracellular loop. *F My cDNA is *F ATGGTAGAGGGCATGATAAAAAAGCTAACTGCTATTTTATTTGGGCTTGTATTGGCTGTGTTATTTCGAGTAATTATACC *F CTTTACTCTGCGACTTAAAAGCGCTGAAATAACCATTAATGTGCTTAACTGTTTTAATTTTCTAACAAGTACCGTTTCCA *F TTACCATTTGCCTGAATTTGGTCAGCCTATACTTAGCCAATTTGCGTGATGATTGGCTTGTTCTCGGGCTTATACCGCTG *F CAGATACTCGTCCTTCATTGGACTTACTTCACAATTTCAAAACAACTAGAAGCCATGAACAGGAGCTATAGTACATTCAT *F GGATAGCTTAAACCTTAAATGGCTGGCATACTCCAATTTTAACGAGACAGATTGGCCTTCGATTGAAAATGCGGTTTTAT *F GCTGTGGACTGGAGGGTCCCCGTTCTTATATGGATTATCTACAAGGGGTTCCAACCCACTGCTACCATCCCGACCTTATC *F ACCCAAGGTTGCAGTGACTTTGTTAAAAACATTTTTATGCCGATACAACATATAAGTCATTTGCAGCTCAGATTGGCTAT *F TTTTGTGGAACTGGTAATTTTACTTATTCTTGCTGCAACGCTTTTGAAGAAATGTATTTCTTTGATCGGCGAAAGGAAAC *F ATAAAAGAGTTATTACACACGGGCTTGCGGTACTAATAGATTTAGTTAAAAACACATTTTGA *F My translation is *F MVEGMIKKLTAILFGLVLAVLFRVIIPFTLRLKSAEITINVLNCFNFLTSTVSITICLNLVSLYLANLRDDWLVLGLIPL *F QILVLHWTYFTISKQLEAMNRSYSTFMDSLNLKWLAYSNFNETDWPSIENAVLCCGLEGPRSYMDYLQGVPTHCYHPDLI *F TQGCSDFVKNIFMPIQHISHLQLRLAIFVELVILLILAATLLKKCISLIGERKHKRVITHGLAVLIDLVKNTFZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128967 *a Ashburner *b M. *t 2000.4.15 *T personal communication to FlyBase *u FlyBase error report for CG18324 on Sat Apr 15 09:10:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 15 17:05:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 15 Apr 2000 17:05:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 15 Apr 2000 09:10:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 582 *F Error report from M. Ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG18324 *F Missed gene *F Comments: David Nelson (pc to FB 10 April 200) gives this translation: *F MTKSDFVLGGTAAMGAVVFTNPIDVVKTRMQLQGELAARGTYVKPYRHLPQAMLQIVLND *F GLLALEKGLAPALCYQFVLNSVRLSVYSNALELGYLQNADGSISFYRGMFFGALGGCTGT *F YFASPFYMIKAQQHAQAVQSIAVGFQHKHTSMMDALLHIYRTNGISGFWRAALPSLNRTL *F VASSVQIGTFPKAKSLLKDKGWITHPVLLSFCAGLSSGTLVAVANSPFDVLTTRMYNQPV *F DEKGRGLMYKGLVDCFTKIWRTEGIHGMYKGFWPIYFRSAPHTTLTFVFFEKLLHLRDRY *F VFSQRRN* *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128968 *a Ashburner *b M. *t 2000.4.15 *T personal communication to FlyBase *u FlyBase error report for CG12201 on Sat Apr 15 09:17:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 15 17:12:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 15 Apr 2000 17:12:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 15 Apr 2000 09:17:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 606 *F Error report from M.Ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG12201 *F Missed gene *F Comments: David Nelson, pc to FB April 10 2000 has a more 5' start of *F translation: *F MLEQVEQKNQEQKKPQKFNVFPKIINGGVAGIIGVACVYPLDMVKTRLQNQTIGPNGERM *F YTSIADCFRKTIASEGYFGMYRGSAVNIVLITPEKAIKLTANDFFRYHLASDDGVIPLSR *F ATLAGGLAGLFQIVVTTPMELLKIQMQDAGRVAAADRAAGREVKTITALGLTKTLLRERG *F IFGLYKGVGATGVRDITFSMVYFPLMAWINDQGPRKSDGSGEAVFYWSLIAGLLSGMTSA *F FMVTPFDVVKTRLQADGEKKFKGIMDCVNRTLKEEGISAFFKGGLCRIMVLAPLFGIAQM *F FYFLGVGEKILGIERTKSV* *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128969 *a Ashburner *b M. *t 2000.4.15 *T personal communication to FlyBase *u FlyBase error report for CG1326 on Sat Apr 15 09:28:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 15 17:24:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 15 Apr 2000 17:24:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 15 Apr 2000 09:28:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 869 *F Error report from M.Ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG1326 *F Missed gene *F Comments: I wonder if this is a fusion between two or more genes. David Nelson *F has this *F as a mitochondrial carrier (pc to FB 10 April 2000): *F AC019525 200827-200898, 200975-201035, 201914-202725 *F 2 introns shown by \* = AC010700 *F ESTs AI512559, AA697689, AI259003, AI532563 *F MVAPSKLSQVLSYQNFVHAVSGAA*GGCIAMSTFYPLDTVRSRLQL*EEAGDVRSTRQVI *F KEIVLGEGFQSLYRGLGPVLQSLCISNFVYFYTFHALKAVASGGSPSQHSALKDLLLGSI *F AGIINVLTTTPFWVVNTRLRMRNVAGTSDEVNKHYKNLLEGLKYVAEKEGIAGLWSGTIP *F SLMLVSNPALQFMMYEMLKRNIMRFTGGEMGSLSFFFIGAIAKAFATVLTYPLQLVQTKQ *F RHRSKESDSKPSTSAGSTPRTESTLELMISILQHQGIRGLFRGLEAKILQTVLTAALMFM *F AYEKIAGTVGMLLKRN* *F This CG is far too long to be a carrier and the IP carrier domain in C terminal *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128970 *a Robertson *b H. *t 2000.4.15 *T personal communication to FlyBase *u FlyBase error report for CG18436 on Sat Apr 15 23:26:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Apr 16 07:21:44 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 16 Apr 2000 07:21:44 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 15 Apr 2000 23:26:19 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 2343 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG18436 *F Gene annotation error *F Gene CG18436 has incorrect exon/intron structure. *F Comments: This is a large gene encoding a large multidomain extracellular *F protein which is the homolog of the moth Manduca sexta lacunin protein we *F described recently (GenBank AF078161) (Nardi, J. B., R. Martos, K. K. O. *F Walden, D. J. Lampe and H. M. Robertson. 1999. Expression of lacunin, a large *F multidomain extracellular matrix protein, accompanies morphogenesis of *F epithelial monolayers in Manduca sexta. Insect Biochemistry and Molecular *F Biology 29, 883-897.) *F By comparison with lacunin, I have three modifications to suggest to the *F annotation. I should emphasize that the two protein products you annotate are *F quite possible, indeed we found evidence for at least two alternative splicing *F events in the moth lacunin, and the nematode homolog is also alternatively *F spliced. *F Nevertheless, with three minor changes, each of which is fully justifiable as *F being more appropriate in terms of the introns involved, one can annotate it *F to encode a good homolog of the moth protein (it has one fewer lagrin domains, *F one more Kunitz domain, and one more immunoglobulin domain than the moth *F protein, but these are minor differences given the alignability of the rest of *F the protein, and the alternative splices in the moth involve addition of a *F Kunitz domain anyway). *F So, I would remove intron 4, which is not well predicted and is an open *F reading frame \- this encodes part of a lagrin domain. I would adjust intron *F 10 from phase 0 to phase 1, which most of the other introns are, and uses a *F better 5' splice site. I would then translate the rest of the cDNA as *F annotated, except that I don't believe the last three exons are part of the *F gene (the splice to them is very poor) and so would end the protein in intron *F 17, which aligns well with lacunin (for which we cloned many overlapping cDNAs *F to complete \- 13kbp!) *F Unfortunately when I try to send you my cDNA it says the 'URI is too long', so *F here is my intron 10 *F gtaacatatacagcgggcgggtctcgacttgggggtcaccatggagtcgccgggctaactggttcttctcttgcgtaag *F and I would end the cDNA barely into intron 17, *F GTGGCCTCGCCTCCTCTGCATCCCAATGCGCTTTACAATGTTTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128971 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG12665 on Sun Apr 16 18:25:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:21:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:21:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:25:42 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1148 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12665 *F Gene annotation error *F Gene CG12665 has incorrect exon/intron structure. *F Comments: This gene encodes a member of the odorant binding protein family, *F which are secreted proteins, it therefore needs a 5' exon encoding a signal *F peptide, and cDNA GH24525 indicates it is as below. *F My cDNA is *F ATGATGCGGAGATCACAGATCGGTTTGCTCAGCAGGCTGCTGCTGTTGCTGCTGGTGGTGGAACTGACGCCCCCTGCTAT *F TCCGGTGCCCATGCGATCCTCACCCCAATCGCTGGCCCTACTGCGAGCACGGGATCAGTGCGGCAGGGAGCTGACTGCTG *F CCCAGCGTCTGCAGCTGGACAGGATGCAATTCGAGGATGCTGCCCATGTGCGTCACTATCTCCATTGCTTCTGGTCACGG *F CTGCAGCTCTGGCTGGATGAGACCGGATTCCAGGCACAGCGCATCGTTCAGAGTTTCGGCGGCGAGAGGCGTCTCAATGT *F GGAGCAGGCACTGCCAGCCATCAACGGGTGCAATGCGAAAACGAGCTCCAGAGGATCGGGCGCTCAGACAGTGGTCGACT *F GGTGTTTCCGTGCCTTTGTCTGCGTGCTGGCCACTCCAGTCGGTGAGTGGTACAAGCGCCACATGTCCGATGTCATCAAT *F GGGAATGCCTAG *F My translation is *F MMRRSQIGLLSRLLLLLLVVELTPPAIPVPMRSSPQSLALLRARDQCGRELTAAQRLQLDRMQFEDAAHVRHYLHCFWSR *F LQLWLDETGFQAQRIVQSFGGERRLNVEQALPAINGCNAKTSSRGSGAQTVVDWCFRAFVCVLATPVGEWYKRHMSDVIN *F GNAZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128972 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG15129 on Sun Apr 16 18:32:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:27:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:27:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:32:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1437 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15129 *F Gene annotation error *F Gene CG15129 should be split. *F Comments: As annotated, this gene encodes two odorant binding proteins. My *F cDNAs are *F ATGGCATTGCTTTGCCTCACTCTTAGTGAATTCGTTTCTAAAGCATGGACCCGATCGCTTTCCGTCTCGCTGAACATGTC *F GATGACACGAACCCTGGTTCCAGATCCGCCAAATGGAACAGAAAACAAACTCAGCCAGGAGATGCTGAGGGCTTGTATGC *F GTAGGACCGAGATCTCAATGTCGCAACTGAAACTCTTTCACATGAGCCTGATGAACAGCGACTACAATAATGACAACGAT *F ATAGCCCCTACGCCAGTTCAATCCATTGGCGATGTAAATAACCTGGGTGATCTGGACTTCAATGGCAACTCGCAGATGCC *F ATATCTCGATCTGAAGCATAATGAGCCGCTGCAGTGCTTTGTGAGCTGTCTGTATGAGACCCTGGATTTGGATAGGTACA *F ATGTCCTGCTGGAGGAGGCCTTTAAGAATCAGGTGCAAACGATCATACAGCATGAGAAGGCGGAGATCAAGGAGTGTAGT *F GATCTTCAGGGCAAAACACGATGCGAGGCAGCCTACAAGCTGCACCTGTGCTACAATCACCTGAAAACTCTGGAGGCGGA *F GCAGCGTATCCGTGAGATACTTGAGCGGACCGAGGCGGAGAACGAGGGATTCGGTCCGGAGGGCAGCGACTTTATCGACG *F GCATCCAGCATTCCGGAGAAGCAATGACCACCGCTAAGTCGGAGTAA *F And *F ATGAAACTTATCTACTTGTTGGTTGTATTCCTAATTTTCGCTCTAAGCGAACTAGTAGCGGGCCAGTCAGCTGCGGAATT *F GGCAGCCTACAAGCAAATTCAACAGGCCTGCATCAAGGAGCTGAATATTGCTGCCAGTGATGCTAATTTGCTGACCACCG *F ACAAGGAGGTGGCGAATCCCTCTGAGTCGGTGAAGTGCTATCACAGCTGCGTCTACAAGAAACTGGGTCTCCTGGGTGAC *F GATGGAAAGCCCAATACTGATAAGATCGTTAAGTTGGCCCAGATCCGTTTCAGCAGTCTGCCGGTGGATAAGCTAAAGAG *F TTTGCTTACCAGCTGCGGAACCACAAAGTCAGCCGCCACCTGTGACTTTGTCTACAACTATGAAAAGTGTGTTGTTAAGG *F GTATTAGTGCCTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128973 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report on Sun Apr 16 18:35:04 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:30:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:30:24 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:35:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 809 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: AE003584.1 contains a gene encoding an odorant binding protein. My *F cDNA is *F ATGCGAGTGTTGCTGGCTTTTGTACTTCTGCTTGGCCTCTCAGTTTTGGCCACTAAGGAACCGGAAGAAGTTAAAATTGT *F AAGCGAGTGTGCCAAGGAGAACAATGTTCATAGGAAGAAGGCACTGGACCTTTTAATGAGCTATCGTTTGAAGAAGAAAA *F CCCACAACGTCATGTGCTTCATCAACTGCATCTTCGAGCGAACCAACATACTGCAGAAAGTTAAGGAAAAAGTTGTAAAG *F GAAAATCACAACTGCGACTCCATCAAGGACGCTGATAAGTGTGCAGAATCCTTCCAAAAATTTCAATGCTTGGTCAAGAT *F TGAGATGAAACGTGATAGTGCAGCAACGCGACATGTGCCACAAACAATGCCCAAACTTTAA *F My translation is *F MRVLLAFVLLLGLSVLATKEPEEVKIVSECAKENNVHRKKALDLLMSYRLKKKTHNVMCFINCIFERTNILQKVKEKVVK *F ENHNCDSIKDADKCAESFQKFQCLVKIEMKRDSAATRHVPQTMPKLZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128974 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report on Sun Apr 16 18:36:35 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:32:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:32:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:36:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 772 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new gene *F Missed gene *F Comments: AE003812.1 has a new gene encoding an odorant binding protein. My *F cDNA is *F ATGAAAGTATTCATTGGCCTGGTTCTGTTGTTAGCTGTCACTACGCTGTCATCCGCTTTATTCGAATCTGAAGCGAACGA *F ATGTGCTAAAAAACTGGGAATTACCCCAGATTACTTCGAAAATTTTCCGCACAGCAGTCGGGTGAAGTGCTTTTACCACT *F GCCAAATGGAAAAACTTGAAATAATTGCCAATGGTGTGGTAACACCATTCGATTTGAAAGTATTGAACATATCACCGGAG *F AGCTATGATAAGTATGGTGTAAAGGTAAAACCATGCCTCAAACTATCGCATCGCGACAAATGTGAGCTCGGTTACTTGGT *F GTTCCAGTGCTTGAAACGAGAATTTAACTTGTAA *F My translation is *F MKVFIGLVLLLAVTTLSSALFESEANECAKKLGITPDYFENFPHSSRVKCFYHCQMEKLEIIANGVVTPFDLKVLNISPE *F SYDKYGVKVKPCLKLSHRDKCELGYLVFQCLKREFNLZ *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128975 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG18111 on Sun Apr 16 18:42:35 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:38:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:38:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:42:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 689 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG18111 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG18111 *F ENIHQQLVGNHADHNEAFHASLAACVDKNEQGSNACEWAYRGATCLLKENLAQIQKSLAPKA . *F Comments: For some reason this accurate cDNA is not fully translated. It *F encodes an odorant binding protein, which are small secreted proteins, and the *F first exon encodes a signal peptide. My translation is *F MKVFVAICVLIGLACADYVVKNRHDMLAYRDECVKELAVPVDLVEKYQKWEYPNDAKTQCYIKCVFTKWGLFDVQSGFNV *F ENIHQQLVGNHADHNEAFHASLAACVDKNEQGSNACEWAYRGATCLLKENLAQIQKSLAPKA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128976 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG1670 on Sun Apr 16 18:44:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:40:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:40:09 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:44:46 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 700 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG1670 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG1670. *F Comments: This gene encodes an odorant binding protein, which are small *F secreted proteins. The annotated cDNa is probably too long at the 5' end (see *F GH13261) and the translation should therefore have a much shorter N-terminus, *F with a clean signal sequence. My translation is *F MTNLLLAVACAAVLMGSATADEEEGSMTVDEVVELIEPFGDACTPKPSRENIVEMVLNKEDAKHETKCFRHCMLEQFELM *F PEDQLQYNEDKTVDMINMMFPDREDDGRRIVKTCNEELKAEQDKCEAAHGIAMCMLREMRSSGFKIPEIKE *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128977 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG11748 on Sun Apr 16 18:47:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:42:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:42:47 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:47:19 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 597 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG11748 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG11748. *F Comments: This gene encodes an odorant binding protein, and the translation *F would be more appropriate if it started a little later, which gives a better *F signal peptide. Mine is *F MKFHLLLVCVAISLGPIPQSEAGVTEEQMWSAGKLMRDVCLPKYPKVSVEVADNIRNGDIPNSKDTNCYINCILEMMQAI *F KKGKFQLESTLKQMDIMLPDSYKDEYRKGINLCKDSTVGLKNAPNCDPAHALLSCLKNNIKVFVFP *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128978 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG15583 on Sun Apr 16 18:57:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 02:52:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 02:52:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 18:57:00 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 2248 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15583 *F Gene annotation error *F Gene CG15583 should be split. *F Comments: This gene as annotated encodes at least two odorant binding *F proteins. The first cDNA is *F ATGCAGTCCCAATCCCTCCTGCTGATCGTTGCAGCCGTTGCCACGTTTCTGGTGGCCCAGACTACGGCCAAGTTCCTGCT *F GAAGGACCACGCCGACGCAGAGAAGGCGTTCGAGGAGTGCCGTGAGGACTACTACGTGCCGGACGACATCTACGAGAAGT *F ACCTGAACTACGAGTTTCCCGCCCACCGGCGCACCAGCTGCTTCGTCAAGTGCTTCCTGGAGAAGCTTGAGCTGTTCTCG *F GAGAAGAAGGGATTTGACGAGCGCGCCATGATCGCCCAGTTCACCTCCAAGAGCAGCAAAGACCTGTCCACGGTCCAGCA *F CGGCCTGGAGAAGTGCATCGACCACAACGAGGCCGAGTCGGATGTCTGCACCTGGGCCAACCGAGTCTTCTCCTGCTGGC *F TGCCCATCAACCGCCACGTGGTGCGTAAGGTCTTCGCCTGA *F encoding *F MQSQSLLLIVAAVATFLVAQTTAKFLLKDHADAEKAFEECREDYYVPDDIYEKYLNYEFPAHRRTSCFVKCFLEKLELFS *F EKKGFDERAMIAQFTSKSSKDLSTVQHGLEKCIDHNEAESDVCTWANRVFSCWLPINRHVVRKVFAZ *F The second half is more problematic, as it appears to encode two odorant *F binding proteins fused together (there is no cryptic intron to split them *F apart). My best cDNA is *F ATGAGTTCCCCTCGTGCGGTTCTAGTCAGCCTGTTCCTGATCTGCAGTCAGGCACTAGCTGACCTTTCTGGTGATGCCCA *F GACTCTGGAAAAGTGCCTGCGGCAACTTAGTTCGCCGGAGAGCATTGCTGGCGATCTTCGAAAGCTGGAACGGTATTCAT *F CTTGGACGCGGGAGGAGGTACCTTGCCTGATGCGCTGCTTGGCCAGAGAAAAGGGCTGGTTCGACGTGGAGGAAAACAAG *F TGGAGGCTCAAGCAACTGACTGAGGACCTGGGCGCCGATGTCTATAACTACTGCAGATTCGAGCTGCGCCGGATGGGGTC *F CGATGGCTGCAGCTTCGCCTATCGGGGACTCAGGTGCCTGAAGCAGGCCGAGATGCATGCGGGCACCAGCCTGAGCACAC *F TGCTACAGTGTTCCCGCCAGCTGAACGCCACCAACGTGGAGCTGCTGCAGTACAGTAAGCTGAAGTCAAAGGAACCTATT *F CCCTGCCTCTTTCAGTGCTTTGCGGATGCCATGGGATTCTACGATCCCGATGGAAACTGGCGGCTGGAAAACTGGAAGCA *F GGCGTTTGGGCCTTCCGGAAATGAGGATCAGTCTTCTGGCGCTGACTACAGTGGCTGTCGACTAAGTGGGACTCAGCGGG *F AGGTGGCGCTAAGCAAGTGCTCGTGGATGTACCATGAGTACAAATGCTGGGAGCGAGTAAATGGGAATAAGCTAGTGGAG *F GACAACGAAGAGCAGTAG *F encoding *F MSSPRAVLVSLFLICSQALADLSGDAQTLEKCLRQLSSPESIAGDLRKLERYSSWTREEVPCLMRCLAREKGWFDVEENK *F WRLKQLTEDLGADVYNYCRFELRRMGSDGCSFAYRGLRCLKQAEMHAGTSLSTLLQCSRQLNATNVELLQYSKLKSKEPI *F PCLFQCFADAMGFYDPDGNWRLENWKQAFGPSGNEDQSSGADYSGCRLSGTQREVALSKCSWMYHEYKCWERVNGNKLVE *F DNEEQZ *F OBP structure has recently been resolved as a dimer, so perhaps this is a *F novel evolutionary arrangement that enforces a heterodimer. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128979 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG13429 on Sun Apr 16 19:25:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:21:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:21:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:25:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 814 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13429 *F Gene annotation error *F Gene CG13429 has incorrect exon/intron structure. *F Comments: I would add a 5' exon with a new N-terminus and remove much of the *F C-terminus from this small secreted odorant binding protein. *F My cDNA is *F ATGTTGGACCAACTTACACTGTGTTTGTTGCTAAATTTTCTGTGCGCAAATGTTCTCGCTAACACTTCAGTATTTAATCC *F GTGTGTTTCGCAAAATGAGTTATCCGAATATGAAGCCCACCAAGTGATGGAGAATTGGCCAGTTCCGCCCATCGATCGGG *F CTTACAAATGCTTTCTAACATGCGTCCTCTTGGATTTGGGTCTGATTGATGAACGGGGTAATGTGCAGATCGATAAGTAC *F ATGAAATCCGGAGTGGTGGACTGGCAATGGGTGGCAATAGAGTTGGTAACATGTCGCATAGAATTCAGCGACGAAAGGGA *F TCTGTGCGAGCTATCATATGGAATCTTCAACTGCTTCAAGGATGTGAAGCTTGCGGCCGAGAAGTATGTTTCAATTAGTA *F ATGCAAAGTAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128980 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG15883 on Sun Apr 16 19:30:40 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:26:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:26:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:30:40 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 784 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15883 *F Gene annotation error *F Gene CG15883 has incorrect exon/intron structure. *F Comments: I would replace the 5' exon of this odorant binding protein with a *F closer one that encodes an appropriate signal sequence. *F My cDNa is *F ATGAAGGTTGTGTGCAGCATAGCTGTACTATGGATTTGCTTGATAACTATGTGGCAATCAGCTGGCCGCGTTAACGCAGA *F GGGTTGCCTAAAGCACCACAATCTGACCAGTGCCCAAGTGCAGGCAGTGGCTCCATCCACTCCCGTTGCGGATGTTCCAG *F TGGCCGTTAAGTGCTATAGCCGGTGTCTGATCCAGGATTATTTCGGTGATGATGGGAAAATCGATCTGCAGAAGGTGGGA *F AAGCGAGGATCTCAAGAGGACCACGTGATTTTGTCCCAGTGTAAGCAGCAGTTCGATGGCGTCACCAATCTGGACACGTG *F CGACTATCCATACCTGATTCTCCAGTGTTATTTTAAGGGCAAGCAGAGTGGAACTATCGCCTCGTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128981 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG13873 on Sun Apr 16 19:32:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:27:34 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:27:34 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:32:00 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 754 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13873 *F Gene annotation error *F Gene CG13873 has incorrect exon/intron structure. *F Comments: I would add a 5' exon encoding the signal peptide for this odorant *F binding protein. *F My cDNA is *F ATGAGGGCTACATTCGCATTGACTCTGTTGCTCGGCTGCCTTTCAGGAATTTTGGCGCAAGCCAACATAGACAGTTCGGT *F GTCCAAGGAACTGGTGACGGATTGCCTCAAGGAGAACGGTGTCACTCCCCAGGATCTGGCTGACTTGCAATCGGGCAAGG *F TGAAGGCCGAGGATGCCAAGGACAATGTGAAGTGCTCCTCACAGTGCATTCTGGTCAAGAGCGGTTTCATGGACTCCACT *F GGCAAACTGCTGACCGACAAGATTAAGTCTTACTATGCGAACTCGAACTTTAAGGATGTCATCGAAAAGGATTTGGACAG *F GTGCAGCGCGGTCAAGGGGGCCAATGCCTGTGACACTGCCTTCAAGATATTATCCTGCTTCCAGGCAGCCAATTAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128982 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG13874 on Sun Apr 16 19:33:09 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:28:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:28:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:33:09 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 766 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13874 *F Gene annotation error *F Gene CG13874 has incorrect exon/intron structure. *F Comments: I would add a 5' exon nearby to add a signal peptide to this odorant *F binding protein. My cDNA is *F ATGAAGTTCACCCTATTCTGTATTGCTCTGGCAGCTTTTTTGTCCATGGGACAGTGTAATCCGGACTTTCGCCAAATAAT *F GCAACAGTGCATGGAGACCAACCAAGTGACCGAGGCTGATCTCAAGGAGTTCATGGCCAGCGGGATGCAGAGCAGTGCCA *F AGGAGAACCTCAAGTGCTACACCAAGTGCCTGATGGAGAAGCAGGGTCATCTCACCAATGGCCAGTTCAATGCTCAGGCT *F ATGCTCGACACTCTCAAAAATGTGCCTCAGATCAAGGACAAAATGGACGAGATTTCCTCGGGAGTGAATGCCTGCAAGGA *F CATCAAGGGAACCAACGATTGCGACACGGCCTTTAAGGTTACCATGTGCCTGAAGGAGCACAAGGCCATTCCAGGACATC *F ACTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128983 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG13874 on Sun Apr 16 19:33:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:28:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:28:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:33:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 766 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13874 *F Gene annotation error *F Gene CG13874 has incorrect exon/intron structure. *F Comments: I would add a 5' exon nearby to add a signal peptide to this odorant *F binding protein. My cDNA is *F ATGAAGTTCACCCTATTCTGTATTGCTCTGGCAGCTTTTTTGTCCATGGGACAGTGTAATCCGGACTTTCGCCAAATAAT *F GCAACAGTGCATGGAGACCAACCAAGTGACCGAGGCTGATCTCAAGGAGTTCATGGCCAGCGGGATGCAGAGCAGTGCCA *F AGGAGAACCTCAAGTGCTACACCAAGTGCCTGATGGAGAAGCAGGGTCATCTCACCAATGGCCAGTTCAATGCTCAGGCT *F ATGCTCGACACTCTCAAAAATGTGCCTCAGATCAAGGACAAAATGGACGAGATTTCCTCGGGAGTGAATGCCTGCAAGGA *F CATCAAGGGAACCAACGATTGCGACACGGCCTTTAAGGTTACCATGTGCCTGAAGGAGCACAAGGCCATTCCAGGACATC *F ACTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128984 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report on Sun Apr 16 19:35:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:31:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:31:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:35:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 697 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: New gene *F Missed gene *F Comments: I propose a new gene encoding an odorant binding protein in *F AE003791. My cDNA is *F ATGCCTGAAAAAATGTCTCTAAGACTTGTACCGCATCTGGCTTGTATTATTTTTATTTTGGAAATTCAATTTAGAATTGC *F CGATTCTAACGATCCGTGCCCCCATAATCAAGGAATAGACGAAGATATAGCCGAATCAATTCTAGGTGACTGGCCTGCAA *F ATGTGGATTTGACTAGCGTGAAAAGGTCCCACAAGTGTTATGTGACCTGTATTTTGCAATATTACAATATTGTGACCGCT *F TCTGGTGAGATATTTCTGGACAAGTACTACGATACTGGAGTCATTGATGAATTGGCGGTGGCACCCAAAATCAATCGATG *F CCGATATGAGTTTAGAATGGAAACAGATTATTGTAGCCGAATTTTTGCTATATTCAATTGTTTAAGGCAAGAAATATTAA *F CAAAGTCATAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128985 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report on Sun Apr 16 19:38:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:33:41 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:33:41 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:38:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 664 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: New gene *F Missed gene *F Comments: I propose a new gene encoding an odorant binding protein in *F AE003794.1 My cDNA is *F ATGAAAGTATTCCTGTTGTTCATTTTCATCTCTGCTATCTGGCTCCAAGCATTTTGTATGAAATCTTCTGAAAAAATAAA *F AGCCTGCTTGAAACGGCAGCTGGGGTATACAATTACAGAAAATACAAAATTTGATGCTAAAGAAGACTCTCTTCAAAGCA *F AGTGTTTTTATCACTGCTTACTGGAAGTGAAAGGTGTTATTGCAAATGATGCGATCAGTTCGGAGCAACCGAGGAAAGTA *F CTTGAAAAAAAGTATGGCATTACTGACACAGATGAATTGGAAAAGGCTGAAGAAAAGTGTCATTCCATCAAGGCTTCAGG *F AAAATGTGAATTGGGCTACGAAATCTTGAAATGCTATCAGTCCATTACCAAGCATTAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128986 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report on Sun Apr 16 19:41:25 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:36:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:36:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:41:25 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 709 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: New gene *F Missed gene *F Comments: I propose a new gene in AE003794 encoding an odorant binding protein. *F My cDNA is *F ATGCATTTTTTCACCTGCTGTGCATTATTGTTAGTCGTCGTTACATTACCAACATGTTTCGTACAAGCAGGTCCCATTAA *F GGATCAATGCATGGCGGCGGCGGGCATCACAGCACAAGATGTTGCGAATCGTCATGAGACCGACGACCCTGGCCATAGTG *F TCAAGTGCTTTTTCCGCTGTTTTCTAGAAAACATTGGCATTATCGCCGATAACCAGATAATACCCGGTGCTTTTGACCGA *F GTTCTAGGCCATATAGTTACCGCGGAAGCCGTAGAGCGAATGGAAGCGACGTGTAATATGATTAAGAGCGAGACATCCCA *F TGACGAGTCTTGTGAATTCGCCTGGCAAATCTCCGAGTGCTACGAAGGAGTAAGATTATCAGATGTTAAGAAGGGCCAAA *F GAACTCGAAATCACAGAGGATAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128988 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report on Sun Apr 16 19:43:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 03:39:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 03:39:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 19:43:43 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 803 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: New gene *F Missed gene *F Comments: I propose another new gene in AE003792 encoding an odorant binding *F protein. *F My cDNA is *F ATGTTCAACACTAGACTTGCCATTTTTTTGCTTCTTATCGTTGTTTCGCTTAGCCAAGCTAAGGAAAGCCAACCCTTTGA *F CTTTTTCGAAGGAACCTATGACGATTTTATTGATTGTCTGAGAATCAATAATATTACCATTGAAGAGTATGAGAAGTTTG *F ACGATACCGACAATTTGGATAATGTCCTCAAGGAAAATGTCGAACTGAAGCACAAGTGCAACATTAAGTGTCAACTGGAA *F AGAGAGCCAACCAAATGGCTAAATGCTCGGGGTGAAGTCGATCTGAAATCAATGAAAGCAACCAGTGAGACAGCGGTATC *F CATATCAAAGTGCATGGAGAAGGCTCCCCAAGAAACCTGTGCCTACGTCTATAAATTGGTAATATGTGCATTCAAATCCG *F GACATTCAGTCATCAAGTTCGATTCATATGAACAAATACAAGAGGAAACCGCTGGACTAATAGCTGAACAGCAGGCGGAT *F CTGTTTGATTACGATACCATCGATTTATAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128989 *a Robertson *b H. *t 2000.4.16 *T personal communication to FlyBase *u FlyBase error report for CG15582 on Sun Apr 16 20:16:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 04:12:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 04:12:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Apr 2000 20:16:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 674 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15582 *F Gene annotation error *F Gene CG15582 has incorrect exon/intron structure. *F Comments: This gene as annotated encodes two units resembling odorant binding *F proteins. It is conceivable that it indeed encodes a heterodimer of these *F OBPs. Alternatively it might involve alternative splicing to the two major *F exons from a new 5' exon encoding a signal peptide *F (ATGCAGATGAAAAGTGGAATATTAATAGCATTATGTCTATGCCTTTCGTTG). I cannot find a *F suitable 5' exon encoding a signal peptide for the second half of the gene. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128990 *a Millburn *b G. *t 2000.4.17 *T personal communication to FlyBase *u FlyBase error report for CG7347 on Mon Apr 17 03:30:25 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 11:26:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 11:26:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 17 Apr 2000 03:30:25 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 414 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG7347 *F Gene annotation error *F Gene CG7347 corresponds to AF224715 (mus304 (FBgn0002901)) *F Comments: CG7347 (FBgn0036776) corresponds to mus304 (FBgn0002901). *F See following e-mail for ClustalW alignment of AF224715 (mus304) and CG7347. *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Mon Apr 17 11:29:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 11:29:26 \+0100 *F To: flybase-help@morgan.harvard.edu *F Subject: *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 17 Apr 2000 11:33:46 \+0100 *F Content-Length: 15188 *F Here is the alignment of CG7347 (FBgn0036776) and mus304 (FBgn0002901). *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: AF224715 2670 bp *F Sequence 2: CG7347|FBan0007347|CT22653|FBa 2620 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 100 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/13691955967030.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:49780 *F Alignment Score 18892 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/13691955967030.aln *F CLUSTAL W (1.8) multiple sequence alignment *F AF224715 *F AAACAAAGCAAACCGGCAAAATAATCTACGACTTTCCATGGCCAAAAAGT 50 *F CG7347|FBan0007347|CT22653|FBa *F AAACAAAGCAAACCGGCAAAATAATCTACGACTTTCCATGGCCAAAAAGT 50 *F AF224715 *F AACGAAATATAGCGGAAACTGGAGGAACCATGGCCAAGCGCTTTTCTGCC 100 *F CG7347|FBan0007347|CT22653|FBa *F AACGAAATATAGCGGAAACTGGAGGAACCATGGCCAAGCGCTTTTCTGCC 100 *F AF224715 *F ATGAAAGACTTCGCGCGGTGCAAGAAACCGCGCCTGGATGTTAGCGTTAC 150 *F CG7347|FBan0007347|CT22653|FBa *F ATGAAAGACTTCGCGCGGTGCAAGAAACCGCGCCTGGATGTTAGCGTTAC 150 *F AF224715 *F CCGCGGATCAGCGCGACCCAGTCCACCGAGGAACAATTTCGATGGGATTC 200 *F CG7347|FBan0007347|CT22653|FBa *F CCGCGGATCAGCGCGACCCAGTCCACCGAGGAACAATTTCGATGGGATTC 200 *F AF224715 *F TCTGGGACGACGATGACGATGTCATCCTGATGGCCACCCAGCTGGCGGAG 250 *F CG7347|FBan0007347|CT22653|FBa *F TCTGGGACGACGATGACGATGTCATCCTGATGGCCACCCAGCTGGCGGAG 250 *F AF224715 *F GCGGAAATCGAGGCGGAGGAGCGGAAAAAGAAGGGCGGAACAGAGGTGGA 300 *F CG7347|FBan0007347|CT22653|FBa *F GCGGAAATCGAGGCGGAGGAGCGGAAAAAGAAGGGCGGAACAGAGGTGGA 300 *F AF224715 *F CATCGGCAACAGCGAGGTCACCTTCAGCGAATTTGCACCCACTTTTCAGG 350 *F CG7347|FBan0007347|CT22653|FBa *F CATCGGCAACAGCGAGGTCACCTTCAGCGAATTTGCACCCACTTTTCAGG 350 *F AF224715 *F GCTCAACCAGCACACAGCAAATGTTTCCGCCGCCACCGCCGCCACAAAAG 400 *F CG7347|FBan0007347|CT22653|FBa *F GCTCAACCAGCACACAGCAAATGTTTCCGCCGCCACCGCCGCCACAAAAG 400 *F AF224715 *F AAGCCTACTTCCCTGGATATGGATGCGATTTTCGCGGATGATGATGATTT 450 *F CG7347|FBan0007347|CT22653|FBa *F AAGCCTACTTCCCTGGATATGGATGCGATTTTCGCGGATGATGATGATTT 450 *F AF224715 *F CGATTTTCTGGCCGTTACCCTCATGGACAGTGAGCCACAAAAGATGCCGG 500 *F CG7347|FBan0007347|CT22653|FBa *F CGATTTTCTGGCCGTTACCCTCATGGACAGTGAGCCACAAAAGATGCCGG 500 *F AF224715 *F AGCCGAAGACCAGCACAAGTAGGATAACTACCAGCAGCATAAGTGTTCAG 550 *F CG7347|FBan0007347|CT22653|FBa *F AGCCGAAGACCAGCACAAGTAGGATAACTACCAGCAGCATAAGTGTTCAG 550 *F AF224715 *F CAGAAAACCACGACCACGACGACCATCAATGCCACGCAATCCCGCCAGCA 600 *F CG7347|FBan0007347|CT22653|FBa *F CAGAAAACCACGACCACGACGACCATCAATGCCACGCAATCCCGCCAGCA 600 *F AF224715 *F GGAGCATCAGCTAAAGTTTCTCATGGATAGGATTGAAGCCCTTAAGCGGG 650 *F CG7347|FBan0007347|CT22653|FBa *F GGAGCATCAGCTAAAGTTTCTCATGGATAGGATTGAAGCCCTTAAGCGGG 650 *F AF224715 *F AAAACGCGCAGCTGGAAAAGAATTTGGGCGACAGTAAGGAACGCAATGAG 700 *F CG7347|FBan0007347|CT22653|FBa *F AAAACGCGCAGCTGGAAAAGAATTTGGGCGACAGTAAGGAACGCAATGAG 700 *F AF224715 *F ATCAAGTCTGGTGAGGTTTCCTTACTTCGTGATGAGCTGAAGCACTTGCG 750 *F CG7347|FBan0007347|CT22653|FBa *F ATCAAGTCTGGTGAGGTTTCCTTACTTCGTGATGAGCTGAAGCACTTGCG 750 *F AF224715 *F CCAGCAGCTGCAGGCTAGCAAAATGGAAAAGCTGGCTTTGGCCGACGAAA 800 *F CG7347|FBan0007347|CT22653|FBa *F CCAGCAGCTGCAGGCTAGCAAAATGGAAAAGCTGGCTTTGGCCGACGAAA 800 *F AF224715 *F CGAATCGGGATTGCAACAAAAAAGTGGCAGAAGCGGCCAAACAAATTGCG 850 *F CG7347|FBan0007347|CT22653|FBa *F CGAATCGGGATTGCAACAAAAAAGTGGCAGAAGCGGCCAAACAAATTGCG 850 *F AF224715 *F GCCAAGGACATCGAGTTGAAGATTAAGAACGCCGAGTTCTCAAAGTTGAA 900 *F CG7347|FBan0007347|CT22653|FBa *F GCCAAGGACATCGAGTTGAAGATTAAGAACGCCGAGTTCTCAAAGTTGAA 900 *F AF224715 *F GACACAGCAAAAAGCCCATGAAAGAAGCATGAATTCCAGCATGAGCATTT 950 *F CG7347|FBan0007347|CT22653|FBa *F GACACAGCAAAAAGCCCATGAAAGAAGCATGAATTCCAGCATGAGCATTT 950 *F AF224715 *F TACAGGCTGCTCCAGATCCTTTGGAGAAACGCTTATCACTTCGCTTGAAT 1000 *F CG7347|FBan0007347|CT22653|FBa *F TACAGGCTGCTCCAGATCCTTTGGAGAAACGCTTATCACTTCGCTTGAAT 1000 *F AF224715 *F AGGCTTAACATACACAGATCTGTGCCCGGGCTGAAAACCGATAATGGCAG 1050 *F CG7347|FBan0007347|CT22653|FBa *F AGGCTTAACATACACAGATCTGTGCCCGGGCTGAAAACCGATAATGGCAG 1050 *F AF224715 *F TGTGTTCGAATACAGCGAAAATGAGGATCAGACTAAGAAACGAAGAAATC 1100 *F CG7347|FBan0007347|CT22653|FBa *F TGTGTTCGAATACAGCGAAAATGAGGATCAGACTAAGAAACGAAGAAATC 1100 *F AF224715 *F ATTTTGAACTGGAATTAAAGCAACTGCTACTTCATTACGCTCGGCTGCAG 1150 *F CG7347|FBan0007347|CT22653|FBa *F ATTTTGAACTGGAATTAAAGCAACTGCTACTTCATTACGCTCGGCTGCAG 1150 *F AF224715 *F GCAAAGCCGGAATCTGTGGATAACCTCCTGCCAAGAATACTCTCGTCGGT 1200 *F CG7347|FBan0007347|CT22653|FBa *F GCAAAGCCGGAATCTGTGGATAACCTCCTGCCAAGAATACTCTCGTCGGT 1200 *F AF224715 *F CGGCAAGGTATTCACGGAGTTTGCTTCGTATGCTCAAAGTTTGGACTTTC 1250 *F CG7347|FBan0007347|CT22653|FBa *F CGGCAAGGTATTCACGGAGTTTGCTTCGTATGCTCAAAGTTTGGACTTTC 1250 *F AF224715 *F CACACAACTGCATGCTGTATCCCTACAATCCCCACAACCTAGAGGAGGAA 1300 *F CG7347|FBan0007347|CT22653|FBa *F CACACAACTGCATGCTGTATCCCTACAATCCCCACAACCTAGAGGAGGAA 1300 *F AF224715 *F GTTCATCGCATTTCCCTGACCCATCAGAGCTGTTTGTATGATAACGAAAA 1350 *F CG7347|FBan0007347|CT22653|FBa *F GTTCATCGCATTTCCCTGACCCATCAGAGCTGTTTGTATGATAACGAAAA 1350 *F AF224715 *F GGCTGTTCCGCTGCGTCGCTTCATAGCTACCTTGGCTTTGATTTGCCGGC 1400 *F CG7347|FBan0007347|CT22653|FBa *F GGCTGTTCCGCTGCGTCGCTTCATAGCTACCTTGGCTTTGATTTGCCGGC 1400 *F AF224715 *F GAGAGGAAAGGATTTCTAGAGGTCTCACGGAGTGGAAAGAAAATGATCTG 1450 *F CG7347|FBan0007347|CT22653|FBa *F GAGAGGAAAGGATTTCTAGAGGTCTCACGGAGTGGAAAGAAAATGATCTG 1450 *F AF224715 *F GGACTGCTAGATATGGCTATTGAAGCTATCACAAAACTAGGATTTTCCTA 1500 *F CG7347|FBan0007347|CT22653|FBa *F GGACTGCTAGATATGGCTATTGAAGCTATCACAAAACTAGGATTTTCCTA 1500 *F AF224715 *F CGAGGTCGGCCAACACTTTGGATTGCTGGAAGCCTTGACTTCACTCCTAA 1550 *F CG7347|FBan0007347|CT22653|FBa *F CGAGGTCGGCCAACACTTTGGATTGCTGGAAGCCTTGACTTCACTCCTAA 1550 *F AF224715 *F ATAGCCTCCTACAAGAAAATGCCCTTCTTCAACATAACGAGGAGCTACTC 1600 *F CG7347|FBan0007347|CT22653|FBa *F ATAGCCTCCTACAAGAAAATGCCCTTCTTCAACATAACGAGGAGCTACTC 1600 *F AF224715 *F TTCGATCTGCTTAAGCAGTTAGTTTTTACTCGTCCCAGTCCTTGGGTTTT 1650 *F CG7347|FBan0007347|CT22653|FBa *F TTCGATCTGCTTAAGCAGTTAGTTTTTACTCGTCCCAGTCCTTGGGTTTT 1650 *F AF224715 *F TGCTGAGCTTAGCTCCTGCTTTTTAAGCTGCCTTAGACATCCCCAACTTA 1700 *F CG7347|FBan0007347|CT22653|FBa *F TGCTGAGCTTAGCTCCTGCTTTTTAAGCTGCCTTAGACATCCCCAACTTA 1700 *F AF224715 *F TGGATAAAATGTGTGTGAATAGTCCCAAGGACTGCTTTGTATCGGATCGC 1750 *F CG7347|FBan0007347|CT22653|FBa *F TGGATAAAATGTGTGTGAATAGTCCCAAGGACTGCTTTGTATCGGATCGC 1750 *F AF224715 *F GTTCGCTCCGTTTATCGATTTGGTCCCGACTCCTGTTTACTTCAGGTCTA 1800 *F CG7347|FBan0007347|CT22653|FBa *F GTTCGCTCCGTTTATCGATTTGGTCCCGACTCCTGTTTACTTCAGGTCTA 1800 *F AF224715 *F CGCGGGCCTACTCGAGCTATGCTTCTTTAGTGAAACTCCTCTGCGGCAGG 1850 *F CG7347|FBan0007347|CT22653|FBa *F CGCGGGCCTACTCGAGCTATGCTTCTTTAGTGAAACTCCTCTGCGGCAGG 1850 *F AF224715 *F ACTACTTTCAGTTGCTGCTCAAGATCGGAGGAAATCATGTGCGATTCGCG 1900 *F CG7347|FBan0007347|CT22653|FBa *F ACTACTTTCAGTTGCTGCTCAAGATCGGAGGAAATCATGTGCGATTCGCG 1900 *F AF224715 *F TTCGAGTGCTTCAAAAACCCACCGGATTTCATACTTGAAATGCTGCCTTA 1950 *F CG7347|FBan0007347|CT22653|FBa *F TTCGAGTGCTTCAAAAACCCACCGGATTTCATACTTGAAATGCTGCCTTA 1950 *F AF224715 *F CTTTGCCGACGATGGCGACGAGGACTCCAGCGATGGTACTCTGATGAAAA 2000 *F CG7347|FBan0007347|CT22653|FBa *F CTTTGCCGACGATGGCGACGAGGACTCCAGCGATGGTACTCTGATGAAAA 2000 *F AF224715 *F CAGGCACCAGTCTGAGCTTCAACTCCACAGGAGCTGTGCAGGGATCGGTC 2050 *F CG7347|FBan0007347|CT22653|FBa *F CAGGCACCAGTCTGAGCTTCAACTCCACAGGAGCTGTGCAGGGATCGGTC 2050 *F AF224715 *F TCCAATGGATCGACATCAGCGTCTGTTTCGAACCCGAATCAAAATTCAAA 2100 *F CG7347|FBan0007347|CT22653|FBa *F TCCAATGGATCGACATCAGCGTCTGTTTCGAACCCGAATCAAAATTCAAA 2100 *F AF224715 *F TTCAAGTACAACGCAGCGAGGAAAAGGCTGCGAGTGCTATGTAAAGTTGT 2150 *F CG7347|FBan0007347|CT22653|FBa *F TTCAAGTACAACGCAGCGAGGAAAAGGCTGCGAGTGCTATGTAAAGTTGT 2150 *F AF224715 *F GCCTCAGTGCGGTAACAATTGTCTTCCAGGTGATGCACCAATGGATGTTG 2200 *F CG7347|FBan0007347|CT22653|FBa *F GCCTCAGTGCGGTAACAATTGTCTTCCAGGTGATGCACCAATGGATGTTG 2200 *F AF224715 *F CACAGCAGGAAAGCAGGCACCGAAGAAGTTGGCGAGATCTCCCGCATCGC 2250 *F CG7347|FBan0007347|CT22653|FBa *F CACAGCAGGAAAGCAGGCACCGAAGAAGTTGGCGAGATCTCCCGCATCGC 2250 *F AF224715 *F AGTGCACCTTCTGTCCCTTGTCTTTCACGAGTATTACCTCACCTGTCTGT 2300 *F CG7347|FBan0007347|CT22653|FBa *F AGTGCACCTTCTGTCCCTTGTCTTTCACGAGTATTACCTCACCTGTCTGT 2300 *F AF224715 *F TTCGCGACTCCGAGGAGACGACCAAGCATTACCTTAGCCTCATATGCAAC 2350 *F CG7347|FBan0007347|CT22653|FBa *F TTCGCGACTCCGAGGAGACGACCAAGCATTACCTTAGCCTCATATGCAAC 2350 *F AF224715 *F TGGTGGAGCGAGCATGCGAACCTGCTTGGTTTTCAGTCAATACATTTGCG 2400 *F CG7347|FBan0007347|CT22653|FBa *F TGGTGGAGCGAGCATGCGAACCTGCTTGGTTTTCAGTCAATACATTTGCG 2400 *F AF224715 *F TTTGTTAAACCAACTGGTTAAAGCCCATTTCATGCTGAAACCCCTTCATC 2450 *F CG7347|FBan0007347|CT22653|FBa *F TTTGTTAAACCAACTGGTTAAAGCCCATTTCATGCTGAAACCCCTTCATC 2450 *F AF224715 *F TGGAGGCCAAGCCCAATAATCCAGTCAACGACCTCTCCGAATGGAAACGC 2500 *F CG7347|FBan0007347|CT22653|FBa *F TGGAGGCCAAGCCCAATAATCCAGTCAACGACCTCTCCGAATGGAAACGC 2500 *F AF224715 *F ATTGTCAAGAATGCGGATGACCAAAGGGCTGTGAAGTCGGCTGTGACAGT 2550 *F CG7347|FBan0007347|CT22653|FBa *F ATTGTCAAGAATGCGGATGACCAAAGGGCTGTGAAGTCGGCTGTGACAGT 2550 *F AF224715 *F TGATCCCAGCAAATTACTCAACACCGACTTCTTTAGTGCCCTTAAACGGG 2600 *F CG7347|FBan0007347|CT22653|FBa *F TGATCCCAGCAAATTACTCAACACCGACTTCTTTAGTGCCCTTAAACGGG 2600 *F AF224715 *F AGGAGAACACTTTTGAGTAGTTATAGTTAGCTTAAACGTTTTAGTAATAT 2650 *F CG7347|FBan0007347|CT22653|FBa *F AGGAGAACACTTTTGAGTAG------------------------------ 2620 *F AF224715 AAAAAAAAAAAAAAAAAAAA 2670 *F CG7347|FBan0007347|CT22653|FBa \-------------------- # *U FBrf0128991 *a Baehrecke *b E. *t 2000.4.17 *T personal communication to FlyBase *u FlyBase error report for CG18389 on Mon Apr 17 13:22:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 17 21:18:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Apr 2000 21:18:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 17 Apr 2000 13:22:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 581 *F Error report from Eric Baehrecke (baehreck@umbi.umd.edu) *F Gene or accession: CG18389 *F Gene annotation error *F Gene CG18389 corresponds to U25686 (FBgn) *F Comments: Annotation failed to recognize that CG18389 has been previously *F reported in Genbank (U25686) and published (Dev.Biol. 171: 85-97). The *F published gene structure was empirically determined and includes that this *F transcription unit is 9.5 kb long. This error is dwarfed by the quality of *F much of the data \- thanks for your effort! *F Browser: Mozilla/4.72 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128992 *a Roos *b C. *t 2000.4.18 *T personal communication to FlyBase *u FlyBase error report for CG6371 on Tue Apr 18 02:57:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 18 10:52:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Apr 2000 10:52:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 18 Apr 2000 02:57:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 387 *F Error report from Christophe Roos (christophe.roos@helsinki.fi) *F Gene or accession: CG6371 *F Gene annotation error *F Gene CG6371 corresponds to DME133105 (FBgn0028374) *F Comments: The hugin gene has been made public. *F This is an update, not an error *F Thank you for your effort to keep the DB updated. *F ChR *F Browser: Mozilla/4.72 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128993 *a Whitfield *b E. *t 2000.4.18 *T personal communication to FlyBase *u FlyBase error report for CG10844 on Tue Apr 18 04:08:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 18 12:04:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Apr 2000 12:04:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 18 Apr 2000 04:08:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 2117 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10844 *F Gene annotation error *F Gene CG10844 corresponds to Rya-r44F FBgn0011286, not Rya-r76CD FBgn0010268 *F has a mistake in the supporting evidence or functional assignment. *F Comments: From the star coded flat file of the genes file: *F \*a Rya-r44F *F \*x FBrf0126712 == Misra, 2000.4.8, personal communication to FlyBase *F \*E FBrf0126712 == Misra, 2000.4.8, personal communication to FlyBase *F \*q Source for merge of: Rya-r44F CG10844 *F Unfortunately the data in GADFly is wrong and links CG10844 to Rya-r76CD *F The data in the nucleotide accession AE003835 is also wrong. The nucleotide *F sequence is of genomic scaffold 142000013386047 section 45 of 52 and the *F protein_ID AAF59036.1 from this entry is linked to Rya-r76CD, when it should *F be to Rya-r44F. *F I believe the flat file is correct and the other datasets are incorrect *F because scaffold 142000013386047 is on chromosome arm 2R, not 3L, ie 44F *F not 76CD. And the sequence of protein_ID AAF59036.1 aligns to what *F Swiss-prot has for Rya-r76CD (TrEMBL entry Q24321, 509 aa fragment) with *F identical amino acid mismatches as the current entries SP has for Rya-r44F *F (TrEMBL entries Q24498, Q24499, Q24500, and Q24501, 4 alternatively spliced *F isoforms). Please note that I believe Celera have identified a fifth *F possible splice isoform, but with 26 known exons there may well be many more! *F I have not attached the clustals below as the protein is in excess of 5000 *F amino acids, to see the alignments please use this web tool: *F http://decypher.ebi.ac.uk/index_by_algo.htm, select Smith-Waterman *F protein-protein search, paste in the translation of protein_ID AAF59036.1, *F select SP as the dataset to search against and see the results for yourself. *F Because of this incorrect link the data on the FlyBase gene web pages for *F Rya-76CD (see http://flybase.bio.indiana.edu/.bin/fbidq.html?FBgn0010268) is *F wrong, includeing the location given as 44F3-44F5, when it should be 76C-76D. *F I think that is all! *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128994 *a Alvarez *b J. *t 2000.4.18 *T personal communication to FlyBase *u FlyBase error report for CG11763 on Tue Apr 18 08:04:15 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 18 15:59:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Apr 2000 15:59:47 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 18 Apr 2000 08:04:15 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 373 *F Error report from John Alvarez (alvarezj@mail.med.upenn.edu) *F Gene or accession: CG11763 *F cDNA or EST error *F Comments: There is a larger ORF at positions 980-3184 that shows strong *F homology to Genebank \#AF006465 B cell antigen receptor Ig beta associated *F protein 1 (mus musculus). *F Browser: Mozilla/4.72 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128995 *a Cheng *b M. *t 2000.4.18 *T personal communication to FlyBase *u FlyBase error report for CG4760 on Tue Apr 18 14:55:05 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 18 22:50:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Apr 2000 22:50:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 18 Apr 2000 14:55:05 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1225 *F Error report from Mickie Cheng (mcheng@biomail.ucsd.edu) *F Gene or accession: CG4760 *F Gene annotation error *F Gene CG4760 has incorrect exon/intron structure. *F Comments: CG4760 does correspond to the boule (FBgn0011206, U51858) gene. *F However, the annotated sequence is missing two regions encoded by the cDNA. *F The first is the first exon which can be found from 58755...59210. The second *F is the last exon which can be found from 86437...87614. The exon *F 81998...82200 should NOT be included as it does not correspond to any of the *F cDNA sequence. Furthermore, the boundaries of the notated exons 75618...76060 *F and 81731...81762 should be changed to 75619...76060 and 81731...81763 to *F accommadate the addition of the first and last exons to this gene structure. *F Finally, a 10 bp gap is present in the boule cDNA when it is compared to the *F notated exon 75618...76060. The extra 10 bp seems to be a small INTRON at *F 75725...75734. This would imply that exon 75618...76060 should be split into *F two exons: 75619...75724 and 75735...76060. Let me know if there are any *F problems with the corrections. Thanks for your time and attention! *F Browser: Mozilla/4.51 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128996 *a Misra *b S. *c G. *d Millburn *t 2000.4.20 *T personal communication to FlyBase *u FlyBase error report for CG10882 on Thu Apr 20 08:19:38 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 20 16:15:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 20 Apr 2000 16:15:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 20 Apr 2000 08:19:38 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report *F Content-Length: 1433 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG10882 *F Gene annotation error *F Gene CG10882 has incorrect exon/intron structure. *F Comments: > Hi Sima, *F > *F > in looking at the new improved GeneScene I discovered a problem with *F > CG10882: *F > *F > I have discovered a bug in the display of some genes in 2L. *F > The evidence bar in the GeneScene display (in this case orange and *F > green) is going across several genes at once, so instead of several *F > small genes, it looks like there is a big gene. The genes in question *F > are (going in order along the genome clicking on the blue exon/intron *F > boxes): *F > CT30485 = CG10882 <- I guess this is what is causing the problem *F > CT37745 = CG17006 *F > CT30487 = Ser12 *F > CT30489 = CG10882 <- I guess this is what is causing the problem *F > CT37749 = CG17012 *F > CT30467 = CG10882 <- I guess this is what is causing the problem *F > *F > I guess this is whay the evidence bar is going across the genes, its *F > because the different CT's have all been assigned to CG10882 (even *F > though there are other CG's in between them on the same strand) *F > *F > According to the GadFly page there is a 4th CT \- CT30493 that has also *F > been assigned to CG10882. \-ooh when I looked on the GeneScene display *F > its on the other strand (!) Presumably this is an annotation problem, *F > *F > Gillian *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4m) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0128997 *a Greene *b E.A. *t 2000.4.24 *T personal communication to FlyBase *u FlyBase error report for CG5300 on Mon Apr 24 13:39:24 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 24 21:34:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 24 Apr 2000 21:34:58 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 24 Apr 2000 13:39:24 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eagreene@fhcrc.org *F Subject: FlyBase error report *F Content-Length: 650 *F Error report from E A Greene (eagreene@fhcrc.org) *F Gene or accession: CG5300 *F Gene annotation error *F Gene CG5300 has incorrect exon/intron structure. *F Comments: The sequence of the mRNA for this gene is now available gb|AF247500. *F The intron predicted from 180891 to 180989 is actually a part of the *F transcript; rather than ending at 181351 the transcript is encoded in genomic *F sequence through 181766 and includes an exon 181340 to 181419 which is part of *F the CDS, as is the start of the final exon 181486 to 181766. The length of *F the protein is 1048 aas. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128998 *a Dunwell *b J.M. *t 2000.4.26 *T personal communication to FlyBase *u FlyBase error report for CG8000 on Wed Apr 26 04:09:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 26 12:04:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 26 Apr 2000 12:04:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 26 Apr 2000 04:09:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: J.Dunwell@reading.ac.uk *F Subject: FlyBase error report *F Content-Length: 1025 *F Error report from Jim M Dunwell (J.Dunwell@reading.ac.uk) *F Gene or accession: CG8000 *F Missed gene *F Comments: There is an error in designation of some of the intron boundaries. *F Use of EST AI517312 (gi|4420412) which comes from this gene suggests a *F probable four/five exon structure encoding a 178 AA protein most closely *F related to a 198 AA 'submergence induced' protein from rice (gi|3201969) and *F the human version, a 179 AA protein (gi|7023256). Suggested introns boundaries *F include 223822-224029, 224089-224182, 224340-224469, 224530-224609. The *F encoded protein is a member of the cupin gene family (see Dunwell et al, 2000, *F Microbiology and Molecular Biology Reviews 64:153-179) that include germins, *F germin-like proteins, and 7S and 11S seed storage proteins. The active site *F residues of this presumed enzyme comprise the three conserved histidines at *F positions 82,84, and 127 and the glutamate at 88 (using numbering of *F gi|7294807). *F Browser: Mozilla/4.7 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0128999 *a Whitfield *b E. *t 2000.4.27 *T personal communication to FlyBase *u FlyBase error report for CG9594 on Thu Apr 27 03:32:52 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 11:28:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 11:28:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 03:32:52 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 847 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9594 *F Gene annotation error *F Gene CG9594/Chd3 has incorrect exon/intron structure. *F Comments: Chd3 cDNA sequence is in AF007780. *F Ashburner wrote to nucleotide sequence submitter so it is known that *F only the N-terminus of AF007780 corresponds to Chd3 (the C terminus *F is a fragment of Mi-2). This cDNA is a C terminal fragment of Chd3, *F the initiating Met is not sequenced. Celera genomic sequence *F corresponds to position 4 of the cDNA and the upstream known 3 codons *F (SNS) are present in the genomic DNA. There is not a Met in the same *F frame upstream so data suggests the initiating Met is separated by *F intron(s). *F Please update the sequence to locate the true init Met *F thanks! *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129000 *a Whitfield *b E. *t 2000.4.27 *T personal communication to FlyBase *u FlyBase error report for CG8103 on Thu Apr 27 07:37:07 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 15:32:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 15:32:23 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 07:37:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 1051 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8103 *F Gene annotation error *F Gene CG8103/Mi-2 has incorrect exon/intron structure. *F Comments: Mi-2 cDNA has been published and sequenced in AF119716. *F Unfortunately the Celera genomic DNA only matches the cDNA for the first *F 886 amino acids of the cDNA, the cDNA continues to a total of 1982 amino *F acids. *F At the very N-terminus and after the first 886 amino acids there is some *F sequence difference. I have made a preliminary search for the missing *F amino acids but have not found the missing exons. The exons are either *F more kb away than I translated, or the cDNA is in error. *F Could you please attempt to find the missing translation, if you cannot I *F will assume the cDNA is in error. *F For now I will not be merging the TrEMBL entries (cDNA = O97159, Celera *F = Q9VW50), I will wait for reannotation of the region (though I am *F aware this is some months away as Mi-2 is in 76D!). *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129001 *a Russell *b S. *t 2000.4.27 *T personal communication to FlyBase *u FlyBase error report for CG12098 on Thu Apr 27 07:01:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 15:00:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 15:00:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 07:01:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: s.russell@gen.cam.ac.uk *F Subject: FlyBase error report *F Content-Length: 478 *F Error report from Steve Russell (s.russell@gen.cam.ac.uk) *F Gene or accession: CG12098 *F Gene annotation error *F Genes CG12098 and CG15552 should be merged. *F Comments: The C-terminal end of CG15552 corresponds to the N-terminal of *F CG12098, these should be merged to correspond with Seq Accession AJ251580, *F Sox100B. We have determined the sequence of cDNA clones and 5' Race *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129002 *a Bayraktaroglu *b L. *t 2000.4.27 *T personal communication to FlyBase *u FlyBase error report for CG11430 on Thu Apr 27 11:23:35 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 19:18:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 19:18:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 11:23:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 792 *F Error report from Leyla Bayraktaroglu (leyla@morgan.harvard.edu) *F Gene or accession: CG11430 *F Gene annotation error *F Gene CG11430 corresponds to AF188634 (FBgn) *F Comments: The gene olf186 has been split into two genes by FlyBase: olf186-F *F and olf186-M. *F olf186-M is in the first intron of olf186-F, but the CDS of olf186-M begins *F before the CDS of olf186-F. *F olf186-F (accession AF188634) (FBgn to be assigned at next FB update) *F corresponds to CG11430|CT31909. *F The CDS annotated on AE003801.1 has an extra exon and now reads: *F 'complement(join(213271..213807,215268..215423, *F 218822..219718,221605..221967))' *F The correct CDS is 'complement(join(213271..213807,215268..215423, *F 221605..221967))' *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129003 *a Bayraktaroglu *b L. *t 2000.4.27 *T personal communication to FlyBase *u FlyBase error report for CG14489 on Thu Apr 27 11:23:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 19:19:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 19:19:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 11:23:50 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 629 *F Error report from Leyla Bayraktaroglu (leyla@morgan.harvard.edu) *F Gene or accession: CG14489 *F Gene annotation error *F Gene CG14489 corresponds to AF188633 (FBgn0015522) *F Comments: The gene olf186 has been split into two genes by FlyBase: olf186-F *F and olf186-M. *F olf186-M is in the first intron of olf186-F, but the CDS of olf186-M begins *F before the CDS of olf186-F. *F olf186-M (accession AF188633) (FBgn0015522) corresponds to CG14489|CT34200| *F The CDS annotated on AE003801.1 for CG14489 is correct : *F 'complement(224869..225633)' *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129004 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG1063 on Fri Apr 28 03:14:59 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:10:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:10:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:14:59 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 550 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1063 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0010051; *F Itp-r83A; CG1063. *F Comments: Celera Sequence for Itp-r83A is AE003602; AAF52015.1 *F Annotation of DNA is missing 1 known isoform that splices out one exon *F 980-988; VEEETNAEA *F and splices an alternate exon *F 1393-1396; VQHF \-> GVGHSV *F for sequence of isoform see: AJ238949; CAB51853.1 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129005 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG9983 on Fri Apr 28 03:16:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:11:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:11:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:16:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 588 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9983 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0001215; *F Hrb98DE; CG9983. *F Comments: Celera Sequence for Hrb98DE is AE003766; AAF56800.1 and AE003766; *F AAF56801.1 *F Annotation of DNA is missing 2 known isoforms *F 1) *F 1-21; MVNSNQNQNGNSNGHDDDFPQ \-> MGGHDNWNNGQNEEQD *F for sequence of isoform see: M25545; AAA28622.1 *F 2) *F 18-21; MISSING *F for sequence of isoform see: M25545; AAA28621.1 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129006 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG10293 on Fri Apr 28 03:18:15 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:13:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:13:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:18:15 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 556 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10293 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0017397; *F how; CG10293. *F Comments: Celera Sequence for how is AE003737; AAF55952.1 *F Annotation of DNA is missing 1 known isoform *F 370-405; VGAIKQQRRLATNREHPYQRATVGVPAKPAGFIEIQ \-> GGLFAR *F for sequence of isoform see: AF003107; AAB60947.1 *F (please note this sequence is of only the alternative exon *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129007 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG10844 on Fri Apr 28 03:24:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:19:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:19:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:24:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 806 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10844 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0011286; *F Rya-r44F; CG10844. *F Comments: Celera Sequence for Rya-r44F is AE003835; AAF59036.1 *F Annotation of DNA is missing 4 known isoforms *F 1025-1059; VRTLLVYGYVLDPPTGEGTEALLAEAQRLKFAGFR \-> *F SANAPGLRICLGSSDGRRNGGTSGRGTTPQVRRIP *F ISOFORMS 2, 3, 4 AND 5. *F 1115-1145; VTKMHAGSIEHFGVRYEAGDVIGCFIDVKEQ \-> *F EEKVYGGVSESFGKQCGPGDIVGVFLDLADH *F ISOFORMS 3 AND 4 *F 1856-1869; MISSING *F ISOFORMS 4 AND 5). *F for sequence of isoforms see: D17389; BAA04212.1 *F D17389; BAA41469.1 *F D17389; BAA41470.1 *F D17389; BAA41471.1 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129008 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG2671 on Fri Apr 28 03:25:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:21:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:21:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:25:53 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 510 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2671 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0002121; *F l(2)gl; CG2671. *F Comments: Celera Sequence for l(2)gl is AE003590; AAF51570.1 *F Annotation of DNA is missing one further isoform *F 708-708; V \-> F IN ISOFORM P78 *F 709-761; MISSING IN ISOFORM P78 *F for sequence of isoform see: M17022; AAA28672.1 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129009 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG3352 on Fri Apr 28 03:27:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:22:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:22:37 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:27:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 491 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3352 *F Gene annotation error *F Gene ft, FBgn0001075, CG3352 has incorrect exon/intron structure. *F Comments: AE003577, protein_ID AAF51036 is for ft, FBgn0001075, CG3352 *F (CT11259). *F Presently this is annotated as an N-terminal fragment. If you simply extend *F the CDS feature from 112538 to 112541 you will include the stop codon. *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129010 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG4509 on Fri Apr 28 03:30:49 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 11:26:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 11:26:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 03:30:49 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 820 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4509 *F Gene annotation error *F Gene CG4509 has incorrect exon/intron structure. *F Comments: Two adjacent genes CG4509 and CG4655 have cadherin repeats. *F CG4509 has a CDS feature, but CG4655 does not. *F CG4655 has an androgen motif at the N terminus and cadherins at C term. *F CG4509 has cadherins at N term, and sugar transporter and nls motif at C *F term. *F I would like the exon/intron structure to be re-evaluated as I believe *F there may be an internal cadherin protein that overlaps the two currently *F existing genes. No known cadherins have an androgen or sugar transport *F motif so I believe these motifs should not be present on the resulting *F cadherin protein. *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129011 *a Whitfield *b E. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG6395 on Fri Apr 28 05:58:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 13:53:34 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 13:53:34 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 05:58:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 477 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6395 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0004179; *F Csp; CG6395. *F Comments: Celera Sequence for Csp is AE003597; AAF51816.1 and AAF51817.1 *F Annotation of DNA is missing 1 further isoform *F 154-174; MISSING *F for sequence of isoform see: AF057167; AAD09430.1 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129012 *a Millburn *b G. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG9414 on Fri Apr 28 07:19:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 15:14:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 15:14:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 07:19:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report *F Content-Length: 760 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG9414 *F Gene annotation error *F Gene CG9414 corresponds to 'dCAD' *F Comments: The gene referred to as 'dCAD' in Yokoyama et al., 2000, J. Biol. *F Chem. 275(17): 12978-12986 corresponds to CG9414 (Rep4, FBgn0028406). *F In the above paper 'dCAD' is said to correspond to EST HL03573. *F BLAST of the 'Drosophila genome' dataset at the NCBI with the HL03573 sequence *F (AA697985) gives only 1 significant alignment, *F which corresponds to part of the sequence of CG9414. *F Clustal alignment of the the HL03573 sequence (AA697985) and CG9414 sequence *F follows, *F in separate e-mail to flybase-updates *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Fri Apr 28 15:18:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 15:18:45 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: FB-error report: CG9414 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 28 Apr 2000 15:23:38 \+0100 *F Content-Length: 8093 *F Hi, *F here is the alignment of CG9414 (Rep4, FBgn0028406) and HL03573 *F sequence (AA697985) as referred to in FB-error report I just sent *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: AA697985.HL03573 793 bp *F Sequence 2: Rep4|FBgn0028406|CT26706|FBan0 1353 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 99 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/9284956681146.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:15007 *F Alignment Score 5734 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/9284956681146.aln *F CLUSTAL W (1.8) multiple sequence alignment *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F ATGATCAGCTACATTAGAGATGCAATCATGTCACCGACAACACGCAGCAG 50 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F CTCGAATAACAAGAAAAGCAGCCAGCCAACAGGCGAAGTTCTCAAGGATG 100 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F TGAGCTCATCCGAATCCAGGGGAACAGCAGGTGCTCCAATGCGTGGCTAC 150 *F AA697985.HL03573 *F \----------ACAACGAGCGAACGAGGAAATACGGAATCGGAGCCAATTC 40 *F Rep4|FBgn0028406|CT26706|FBan0 *F AAGGTTACTGACAACGAGCGAACGAGGAAATACGGAATCGGAGCCAATTC 200 *F AA697985.HL03573 *F CCTGGAAATGCTGATAGCCAAGGCAAAAAGCAAGTTCCCGCTTCTCGAGC 90 *F Rep4|FBgn0028406|CT26706|FBan0 *F CCTGGAAATGCTGATAGCCAAGGCAAAAAGCAAGTTCCCGCTTCTCGAGC 250 *F AA697985.HL03573 *F CACATTTGTATTTGGCTTCTGATGGCTTCGAGGTGTCCGACGATGAGTAC 140 *F Rep4|FBgn0028406|CT26706|FBan0 *F CACATTTGTATTTGGCTTCTGATGGCTTCGAGGTGTCCGACGATGAGTAC 300 *F AA697985.HL03573 *F TTAAAAAGCCTACCCGCCCAGACGCTGTTCATAGTGTCTGGCCCGGATGC 190 *F Rep4|FBgn0028406|CT26706|FBan0 *F TTAAAAAGCCTACCCGCCCAGACGCTGTTCATAGTGTCTGGCCCGGATGC 350 *F AA697985.HL03573 *F AGTCATTACAACAGATGCCGATTTTGAGTTTGAGAAGATGCGACAACAAT 240 *F Rep4|FBgn0028406|CT26706|FBan0 *F AGTCATTACAACAGATGCCGATTTTGAGTTTGAGAAGATGCGACAACAAT 400 *F AA697985.HL03573 *F CGCCTTTGCTAAAGGTGGCTGACATTTTCTACGATTTTATCGAACAGCAT 290 *F Rep4|FBgn0028406|CT26706|FBan0 *F CGCCTTTGCTAAAGGTGGCTGACATTTTCTACGATTTTATCGAACAGCAT 450 *F AA697985.HL03573 *F CCGGAAAAGTTCCGCCGCATGATAACGGAGTATGAACACCAAAAGCAGCG 340 *F Rep4|FBgn0028406|CT26706|FBan0 *F CCGGAAAAGTTCCGCCGCATGATAACGGAGTATGAACACCAAAAGCAGCG 500 *F AA697985.HL03573 *F ACGCGTCCTGGATAACAGCAAGACCCACCTCAGCTTAAAGGCCGAGCACG 390 *F Rep4|FBgn0028406|CT26706|FBan0 *F ACGCGTCCTGGATAACAGCAAGACCCACCTCAGCTTAAAGGCCGAGCACG 550 *F AA697985.HL03573 *F TGGAGTGGTTCACGGGCGGCGAGGAGC-CTTCCACTCTAAGGAGGAAGCT 439 *F Rep4|FBgn0028406|CT26706|FBan0 *F TGGAGTGGTTCACGGGCGGCGAGGAGCGCTTCCACTCTAAGGAGGAAGCT 600 *F AA697985.HL03573 *F ATGGCCACACGTGCCCAGACAACGTGTGCGAGGCTACTACTATAAGGCCA 489 *F Rep4|FBgn0028406|CT26706|FBan0 *F ATGGCCACACGTGCCCAGACA-CGTGTGCGAGGCTACTACTATAAGGCCA 649 *F AA697985.HL03573 *F AGGAGGAGCTGACCCGCAATCCCTTGTACCGCCAAAATGCCAAGGCCCGA 539 *F Rep4|FBgn0028406|CT26706|FBan0 *F AGGAGGAGCTGACCCGCAATCCCTTGTACCGCCAAAATGCCAAGGCCCGA 699 *F AA697985.HL03573 *F CAAGTGATAAACTCTGTGCTGGAGAAGTTTCGATACCTGCTCATCGGTTG 589 *F Rep4|FBgn0028406|CT26706|FBan0 *F CAAGTGATAAACTCTGTGCTGGAGAAGTTTCGATACCTGCTCATCGGTTG 749 *F AA697985.HL03573 *F CGACTTTTTCTCCATGATGTTTGACCGGAATTGCAAGCAAAAGCATGAAT 639 *F Rep4|FBgn0028406|CT26706|FBan0 *F CGACTTTTTCTCCATGATGTTTGACCGGAATTGCAAGCAAAAGCATGAAT 799 *F AA697985.HL03573 *F TCCTGAAGCAGCACTTGGGCGACGAGGAAACAGACGCTGGCAGGATACCC 689 *F Rep4|FBgn0028406|CT26706|FBan0 *F TCCTGAAGCAGCACTTGGGCGACGAGGAAACAGACGCTGGCAGGATACCC 849 *F AA697985.HL03573 *F AGCAAGAGACTGAGGCAGGTGATTAGGGAGTACACCAAGGAAAACTGCAT 739 *F Rep4|FBgn0028406|CT26706|FBan0 *F AGCAAGAGACTGAGGCAGGTGATTAGGGAGTACACCAAGGAAAACTGCAT 899 *F AA697985.HL03573 *F CCTCGACGAATGGTCCACTTCCTTGTGCTCCGACTTGGGTGACTTCTATT 789 *F Rep4|FBgn0028406|CT26706|FBan0 *F CCTCGACGAATGGTCCACTTCCTTGTGCTCCGACTTGGGTGACTTCTATT 949 *F AA697985.HL03573 *F GCCA---------------------------------------------- 793 *F Rep4|FBgn0028406|CT26706|FBan0 *F GCCAGGGCTCCTATTCGGAGAATGGCAACAGCTGCTCTAAGCAGCACACT 999 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F ATTAATCCGTACGCTTCGCGTGAGAATCTCATTTTGTTTCAGGTCTGGAA 1049 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F TCTGGACCACCAAATCGAACTGTGCCGCACAATCCTTCCCGCACTTGTTG 1099 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F CAAATGTGGAAGAACTTGTGAGTCATCCGCAGACCAAGTGTTCAATTCAT 1149 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F AAGAAGCAAGTGGTCGATATCTCAGTACTGGAGTACTTTCTAGAAATATT 1199 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F CTCCCTAAAGAATCTCAAACTAGTGCACATTGTCTGTCATGAGAAGGCGC 1249 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F AGCGCTCAAACCGTTCGAATGGTCGCCTACTGTGCTCCGACTGTCATGAG 1299 *F AA697985.HL03573 *F \-------------------------------------------------- *F Rep4|FBgn0028406|CT26706|FBan0 *F TATCGCATTGTGCAAGAGCTGATGGAAGTCCAGGTGGACAGTCTAGCCAC 1349 *F AA697985.HL03573 \---- *F Rep4|FBgn0028406|CT26706|FBan0 TTAA 1353 # *U FBrf0129013 *a Sierralta *b J. *t 2000.4.28 *T personal communication to FlyBase *u FlyBase error report for CG12021 on Fri Apr 28 08:47:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 28 16:42:51 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Apr 2000 16:42:51 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Apr 2000 08:47:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jsierral@machi.med.uchile.cl *F Subject: FlyBase error report *F Content-Length: 540 *F Error report from Jimena Sierralta (jsierral@machi.med.uchile.cl) *F Gene or accession: CG12021 *F Gene annotation error *F Gene CG12021 corresponds to AAD43031 (FBgn) *F Comments: The Gene JTBR (Hatakeyama, S, Fbgn 0025820) does not have PDZ *F domains in the predicted protein. The gene Discs Lost do have 4 PDZ domains as *F the report for CG12021 compile and is coded by the (+) strand. The gene JTBR *F correspond to the entry CG1935 that is code by the (-) strand *F Browser: Mozilla/4.72 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129014 *a Whitfield *b E. *t 2000.5.2 *T personal communication to FlyBase *u FlyBase error report for CG1634 on Tue May 2 08:06:07 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 02 16:01:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 May 2000 16:01:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 2 May 2000 08:06:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 621 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1634 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene FBgn0002968; *F Nrg; CG1634. *F Comments: Celera Sequence for Nrg is AE003444; AAF46387.1 *F Annotation of DNA is missing 1 further isoform that splices an *F alternative carboxy terminal exon: *F 1224-1239; QFTEDGSFIGQYVPGK \-> MNEDGSFIGQYGRKGL *F 1240-1302; MISSING *F the DNA is there but I did not work out exact coordinates. *F for sequence of isoform see: AF050085; AAC28614.2 *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129015 *a Kolmer *b M. *t 2000.5.1 *T personal communication to FlyBase *u FlyBase error report for CG8627 on Mon May 1 21:18:11 2000. *F From meelis.kolmer@ktl.fi Mon May 01 19:15:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 1 May 2000 19:15:07 \+0100 *F Date: Mon, 01 May 2000 21:18:11 \+0300 *F From: Meelis Kolmer *F Subject: error report submission FAILS *F To: flybase-help@morgan.harvard.edu *F Reply-To: meelis.kolmer@ktl.fi *F Mime-Version: 1.0 *F X-Mailer: Pegasus Mail for Win32 (v3.12b) *F Content-Transfer-Encoding: 7BIT *F Priority: normal *F . *F . *F . *F Error report from Meelis Kolmer (meelis.kolmer@ktl.fi) *F Gene or accession: CG8627 *F Missed gene *F Comments: *F Dear FlyBase Curators, *F Correct annotation would be: acyl-coa binding protein *F \- binds Acyl-CoA *F \- acts as a transporter and/or pool-former of Acyl-CoA *F Synonyms (aliases) for DBI: *F \- diazepam-binding protein (DBI) *F \- acyl-CoA binding protein (ACBP) *F \- endozepine *F _________________________________ *F 1. Many possible biological roles have been suggested for DBI *F \- binding of Acyl-CoA *F \- stimulation of steroidogenesis through peripheral benzodiazepine *F receptors (also known as mitochondrial benzodiazepine receptors) *F \- modulation of GABA receptor type A (DBI/ACBP protein was first *F purified and also named on the bases of its ability to inhibit *F diazepam binding to GABA receptors of type A) *F \- stimulation of insulin secretion *F \- antibacterial properties *F \- stimulation of cholecystokinin (CCK) secretion *F \- binding of lead (lead-binding protein) *F 2. However the binding of Acyl-CoA is the only function of DBI/ACBP *F which has been supported with remarkable body of experimental evidence. *F 3. Evidence: *F \- extensive biochemical data \- for a reviews: *F Kragelund BB et al. *F Biochim Biophys Acta. 1999 Nov 23;1441(2-3):150-61. Review. *F Knudsen J et. al. *F Mol Cell Biochem. 1999 Feb;192(1-2):95-103. Review. *F \- three-dimensional structure of ACBP/DBI and palmitoyl-CoA has *F been resolved by multidimensional nuclear magnetic resonance (NMR) *F spectroscopy *F Kragelund BB, et al. *F J Mol Biol. 1993 Apr 20;230(4):1260-77. *F \- knock-out experiments in S. cerevisiae *F Schjerling CK et. al. *F J Biol Chem. 1996 Sep 13;271(37):22514-21. *F \- please note that DBI/ACBP has been conserved throughout the *F evolution and is found in unicellular eukaryote S. cerevisiae and *F also in prokaryote Deinococcus radiodurans. Therefore it is highly *F unlikely that the real function of ACBP/DBI would be binding of *F diazepam..... *F 4. Our own studies have shown that in D. melanogaster the expression of *F ACBP/DBI is expressed in the following tissues: *F \- outer vacuolate epithelium of the stomodeal valve of the cardia *F \- in distal part of the anterior Malpighian tubules, *F \- fat body, *F \- gametes of both sexes *F \- pupal and larval brains (no expression in adult CNS). *F Localization and morphological characteristics of structures *F that do express DBI/ACBP suggest that these structures are most *F likely glial cells and their processes. *F Kolmer M, et. al. *F Mol Cell Biol. 1994 Oct;14(10):6983-95. *F 5. On the basis of the expression of DBI in some but not all tissues *F with high energy consumption, we proposed that in D. melanogaster, *F DBI is involved in energy metabolism in a manner that depends on *F the substrate used for energy production (most likely Acyl-CoA, *F however we have no direct experimental evidence so far). *F _________________________________________________ *F Finally, please also see a Swiss-Prot annotation line below *F (entry name: ACBP_BOVIN) *F FUNCTION: BINDS MEDIUM- AND LONG-CHAIN ACYL-COA ESTERS WITH VERY *F HIGH AFFINITY AND MAY FUNCTION AS AN INTRACELLULAR CARRIER OF *F ACYL-COA ESTERS. IT IS ALSO ABLE TO DISPLACE DIAZEPAM FROM THE *F BENZODIAZEPINE (BZD) RECOGNITION SITE LOCATED ON THE GABA TYPE A *F RECEPTOR. IT IS THEREFORE POSSIBLE THAT THIS PROTEIN ALSO ACTS AS A *F NEUROPEPTIDE TO MODULATE THE ACTION OF THE GABA RECEPTOR. *F _______________________________ *F Thank you for your time and for keeping FlyBase up and running. *F with all the best wishes, Meelis *F \--------------------------------------------------------------------- *F Meelis Kolmer phone: \+358-9-4744 8275 *F National Public Health Institute phone: \+358-9-4744 8394 *F Department of Human Molecular Genetics fax: \+358-9-4744 8480 *F Mannerheimintie 166 *F FIN-00300 Helsinki, Finland e-mail: meelis.kolmer@ktl.fi *F \--------------------------------------------------------------------- # *U FBrf0129016 *a Bayraktaroglu *b L. *t 2000.5.1 *T personal communication to FlyBase *u FlyBase error report for CG8919 on Mon May 1 14:39:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 01 22:35:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 1 May 2000 22:35:05 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 1 May 2000 14:39:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 651 *F Error report from Leyla Bayraktaroglu (leyla@morgan.harvard.edu) *F Gene or accession: CG8919 *F Gene annotation error *F Gene CG8919 should be split. *F Comments: CG8919|CT25618 should be split: the region from amino acids 1408-2122 *F of CG8919 corresponds to xmas-1 (FBgn0016080), and the region from aa 1-1359 *F corresponds to xmas-2 (Bgn0028974) (the intron-exon structure of each mRNA/CDS *F needs to be fixed). *F The ClustalW alignments of CG8919|CT25618 CDS to xmas-1 (AAF23814) *F and xmas-2 (AAF23815) CDSs follow, in a separate e-mail to flybase-updates. *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From leyla@morgan.harvard.edu Mon May 01 22:36:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 1 May 2000 22:36:15 \+0100 *F Date: Mon, 1 May 2000 17:41:52 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: FB-error report:CG8919,xmas-1,xmas-2 *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-MD5: gSX5UMeAL5dHLVFXAT+8SQ== *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Below are the ClustalW alignments of xmas-1 (AAF23814) and xmas-2 (AAF23815) *F to CG8919|CT25618. *F Leyla *F ______________________________________________________________________________ *F xmas-1 (FBgn0016080) aligned to CG8919|CT25618 *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: CG8919|FBan0008919|CT25618|FBa 2122 aa *F Sequence 2: gi|6685148|gb|AAF23814.1|AF216 736 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 96 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/27590957214667.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:11730 *F Alignment Score 4377 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/27590957214667.aln *F Your guide tree: *F 27590957214667.dnd *F (CG8919|FBan0008919|CT25618|FBa:0.01630,gi|6685148|gb|AAF23814.1|AF216:0.01630); *F Your Multiple Sequence Alignment: *F 27590957214667.aln *F CLUSTAL W (1.8) multiple sequence alignment *F CG8919|FBan0008919|CT25618|FBa *F MAEPRPGGYNYKTLLCRNIPELFLDKYVARSHFGRFGTLVNFVLRPRRMT 50 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F CTVSYASEDEAARALLDGASFQGHLFDISYADNETAPAQKTEEWVDPDIQ 100 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F AELSALQSGWRNEYGSGKPIKKPQNGSSGSGGSSMLPAIPVGPATAPVSR 150 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F DRTPAQLRDLENMMRRPAHTSEEKFSVLDARDKLLRLNRTQHKLSGATQG 200 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F HCADMCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQE 250 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRS 300 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F IRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENL 350 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPA 400 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F ELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRL 450 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F RVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQ 500 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F INEAGRVVLSRMHTVETEYKLPRQYELVEVKRVKSVGEVVSGEPLPPRDL 550 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F YLNHRPHNSFDDYGMLKSIAWTAKDQLAGMQQEEMQPQMPSQPPAVSKHS 600 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F DTLFKVPMQPDGTAAGFGVFAAAAVPPASSIDGFSFVLPKSRAQEFQEQA 650 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F PATQQRRAQEEAKHQALQVAIAAAKKREAELMAIHEAKVAEAERVRQQKL 700 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F RERQEQQRRQQQELEEQRQREQEKLQLEKERQLKLEQLFFVQQQEREAHK 750 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F QTRTLELYQEIFQDTLAEICQSEFMSHSRACRSYESMLDSITRDLVERQM 800 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F EQSIYELGVMRVCIRRWRKYRRTQQEKDTLFNQLPLSFGAENPEGVVNKR 850 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F SMEDSLRLSRRYRLGEPCDYGKLLAGLEEHSWLKLDLWHVLDKCLPVAQP 900 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F GARRFYKLLISLSGGQEGLQLNCDLDRGLLQQPQSPDARFVDGGYIRGFS 950 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F QGIGLSVMKIRDDDHDWKATDLAEANGIICLIGLDDIRLLPDRLKPLLQA 1000 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F SRCHDVAVIVQHPANTAFVQPDIPLQELCLRSFNIFRLRKSGNNRQRLMI 1050 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F ALESAVKFLAKATERKRVGHLHQVETREYLLVNLGSELFRRLKYAAEHDT 1100 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F AIRSDTQLNPQRCVDLFNEAVHRLQLVAGEDLSDWPQFPEELRVFVQPLP 1150 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F IESSLNTNRLEHFEPGWHLPERRQRIVQLLERCKLPKMPVLPRSSSLAEA 1200 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F QCQWVLDYAQISQQEDCVEQIALQAIKILQYDTDDYLNFVEYLAGERMQY 1250 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F ILRQERNPPQGIVYNTKTLKRRFLSAWYYEFREPQIYEPVPAEENAQMLE 1300 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F KQPSSQAEVQQLDFDEITSKAEAVLKRFHQRQDERHTLRELNRSHKSRKR 1350 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F RDASDHKHATLVTLQSTKQLRGDSIHLSKIFVFPFFFLFCFKVFFYIQNH 1400 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TKGFAWNMKSLVAQTECQLRPLKVSSLVSALKAARSDYQGKSSAVCRLVA 1450 *F gi|6685148|gb|AAF23814.1|AF216 *F \-------MKSLVAQTECQLRPLKVSSLVSALKAARSDHQGKSSAVCRLVA 43 *F CG8919|FBan0008919|CT25618|FBa *F QMVEADQVKYQWTPNLTSLIDRKDASTRHQWRPHVNHSTRHARKLPVQSI 1500 *F gi|6685148|gb|AAF23814.1|AF216 *F QMVEADQVKYQWTPNLTSLIDRKDASTRHQWRPHVNHSTRHARKLPVQSI 93 *F CG8919|FBan0008919|CT25618|FBa *F SFLSRCLAKKGEHAGLTSCVLSVKTRYAPKTLHGRELLIWRQSSMRPLLL 1550 *F gi|6685148|gb|AAF23814.1|AF216 *F SFLSRCLAKKGEHAGLTSCVLSVKTRYAPKTLHGRELLIWRQSSMRPLLL 143 *F CG8919|FBan0008919|CT25618|FBa *F PSSNLWTGVLSKRHYVPRLRPAEEDEEVERRAVPQLRITKEQSEEEEHQL 1600 *F gi|6685148|gb|AAF23814.1|AF216 *F PSSNLWTGVLSKRHYVPRLRPAEEDEEVERRAVPQLRITKEQSEEEEHQL 193 *F CG8919|FBan0008919|CT25618|FBa *F QRRKDGSRLRNECSEFYLPMGEQDYSEREEENRGHRQAYVPSELMTPELA 1650 *F gi|6685148|gb|AAF23814.1|AF216 *F QRRKDGSRLRNECSEFYLPMGEQDYSEREEENRGHRQPYVPSELMTPELA 243 *F CG8919|FBan0008919|CT25618|FBa *F TKWQTTSVTDRDRQLLNLEQQMKTQPSVRMTTQTWFSGNHRRYRSRRSGA 1700 *F gi|6685148|gb|AAF23814.1|AF216 *F TKWQTTSVTDRDRQLLNLEQQMKTQPSVRMTTQTWFSGNHRRYRSRRSGA 293 *F CG8919|FBan0008919|CT25618|FBa *F KRYHVVSELEGWRRSSNHQPVPVFQGQPGEQEHLRHASHKASARSMPDGQ 1750 *F gi|6685148|gb|AAF23814.1|AF216 *F KRYHVVSELEGWRRSSNHQPVPVFQGQPGEQEHLRHASHKASARSMPDGQ 343 *F CG8919|FBan0008919|CT25618|FBa *F E---------------------RCQVRMPRIRAAKSAVMMAQEARNKHHR 1779 *F gi|6685148|gb|AAF23814.1|AF216 *F ERCQMVWEQKKRSPWHRTEVTNSNKVRMPRIRAAKSAVMMAQEARNKHHR 393 *F \* *F CG8919|FBan0008919|CT25618|FBa *F LITKKLVYRPRRTVNAVQAEETEDQDTHHRHHGGGQKMSKRAPERALLKQ 1829 *F gi|6685148|gb|AAF23814.1|AF216 *F LITKKLVYRPRRTVNAVQAEETEDQDTHHRHHGGGQKMSKRAPERALLKQ 443 *F CG8919|FBan0008919|CT25618|FBa *F HLADLLAVSKPEDSFRIGYQPNAPTRQLLEQGKCYVSLRREQFIHLHPVR 1879 *F gi|6685148|gb|AAF23814.1|AF216 *F HLADLLAVSKPEDSFRIGYQPNAPTRQLLEQGKCYVSLRREQFIHLHPVR 493 *F CG8919|FBan0008919|CT25618|FBa *F PNSLILAVNLEVKESPRQPVTTLHRRDGAKRPRLVEDVVKPSLITVIRQS 1929 *F gi|6685148|gb|AAF23814.1|AF216 *F PNSLILAVNLEVKESPRQPVTTLHRRDGAKRPRLVEDVVKPSLITVIRQS 543 *F CG8919|FBan0008919|CT25618|FBa *F AATWDHNGRKVPAKKSNLVGMPSKEHAYQRRLVRNTSTLEAIVKAQAKPR 1979 *F gi|6685148|gb|AAF23814.1|AF216 *F AATWDHNGRKVPAKKSNLVGMPSKEHAYQRRLVRNTSTLEAIVKAQAKPR 593 *F CG8919|FBan0008919|CT25618|FBa *F SEPKSPSSATDYHRVASQKLPHVTSKADISKAADPFKDLDKPRIKEKEVA 2029 *F gi|6685148|gb|AAF23814.1|AF216 *F SEPKSPSSATDYHRVASQKLPHVTSKADISKAADPFKDLDKPRIKEKEVA 643 *F CG8919|FBan0008919|CT25618|FBa *F SSWKQAYVVRSKMYDAITPYRRRAYPGKKCITKDKTDGYFLAKRSSAAAA 2079 *F gi|6685148|gb|AAF23814.1|AF216 *F SSWKQAYVVRSKMYDAITPYRRRAYPGKKCITKDKTDGYFLAKRSSAAAA 693 *F CG8919|FBan0008919|CT25618|FBa *F GSSKKVKPPKQPVVSPKVQVPSVLHKKKSRESLGPQTTKTGKL 2122 *F gi|6685148|gb|AAF23814.1|AF216 *F GSSKKVKPPKQPVVSPKVQVPSVLHKKESRESLGPQTTKTGKL 736 *F ______________________________________________________________________________ *F xmas-2 (Bgn0028974) aligned to CG8919|CT25618| *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|6685149|gb|AAF23815.1|AF216 1184 aa *F Sequence 2: CG8919|FBan0008919|CT25618|FBa 2122 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 98 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/28081957214859.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:19364 *F Alignment Score 7329 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/28081957214859.aln *F Your guide tree: *F 28081957214859.dnd *F (gi|6685149|gb|AAF23815.1|AF216:0.00802,CG8919|FBan0008919|CT25618|FBa:0.00802); *F Your Multiple Sequence Alignment: *F 28081957214859.aln *F CLUSTAL W (1.8) multiple sequence alignment *F gi|6685149|gb|AAF23815.1|AF216 *F MAEPRPGGYNYKTLLCRNIPELFLDKYVARSHFGRFGTLVNFVLRPRRMT 50 *F CG8919|FBan0008919|CT25618|FBa *F MAEPRPGGYNYKTLLCRNIPELFLDKYVARSHFGRFGTLVNFVLRPRRMT 50 *F gi|6685149|gb|AAF23815.1|AF216 *F CTVSYASEDEAARALLDGASFQGHLFDISYADNETAPAQKTEEWVD---- 96 *F CG8919|FBan0008919|CT25618|FBa *F CTVSYASEDEAARALLDGASFQGHLFDISYADNETAPAQKTEEWVDPDIQ 100 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F AELSALQSGWRNEYGSGKPIKKPQNGSSGSGGSSMLPAIPVGPATAPVSR 150 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F DRTPAQLRDLENMMRRPAHTSEEKFSVLDARDKLLRLNRTQHKLSGATQG 200 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F HCADMCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQE 250 *F gi|6685149|gb|AAF23815.1|AF216 *F \--------------------------------PQSHMGDWFHFVWDRTRS 114 *F CG8919|FBan0008919|CT25618|FBa *F TPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRS 300 *F gi|6685149|gb|AAF23815.1|AF216 *F IRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENL 164 *F CG8919|FBan0008919|CT25618|FBa *F IRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENL 350 *F gi|6685149|gb|AAF23815.1|AF216 *F TKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPA 214 *F CG8919|FBan0008919|CT25618|FBa *F TKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPA 400 *F gi|6685149|gb|AAF23815.1|AF216 *F ELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRL 264 *F CG8919|FBan0008919|CT25618|FBa *F ELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRL 450 *F gi|6685149|gb|AAF23815.1|AF216 *F RVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQ 314 *F CG8919|FBan0008919|CT25618|FBa *F RVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQ 500 *F gi|6685149|gb|AAF23815.1|AF216 *F INEAGRVVLSRMHTVETEYKLPRQYELVEVKRVKSVGEVVSGEPLPPRDL 364 *F CG8919|FBan0008919|CT25618|FBa *F INEAGRVVLSRMHTVETEYKLPRQYELVEVKRVKSVGEVVSGEPLPPRDL 550 *F gi|6685149|gb|AAF23815.1|AF216 *F YLNHRPHNSFDDYGMLKSIAWTAKDQLAGMQQEEMQPQMPSQPPAVSKHS 414 *F CG8919|FBan0008919|CT25618|FBa *F YLNHRPHNSFDDYGMLKSIAWTAKDQLAGMQQEEMQPQMPSQPPAVSKHS 600 *F gi|6685149|gb|AAF23815.1|AF216 *F DTLFKVPMQPDGTAAGFGVFAAAAVPPASSIDGFSFVLPKSRAQEFQEQA 464 *F CG8919|FBan0008919|CT25618|FBa *F DTLFKVPMQPDGTAAGFGVFAAAAVPPASSIDGFSFVLPKSRAQEFQEQA 650 *F gi|6685149|gb|AAF23815.1|AF216 *F PATQQRRAQEEAKHQALQVAIAAAKKREAELMAIHEAKVAEAERVRQQKL 514 *F CG8919|FBan0008919|CT25618|FBa *F PATQQRRAQEEAKHQALQVAIAAAKKREAELMAIHEAKVAEAERVRQQKL 700 *F gi|6685149|gb|AAF23815.1|AF216 *F RERQEQQRRQQQELEEQRQREQEKLQLEKERQLKLEQLFFVQQQEREAHK 564 *F CG8919|FBan0008919|CT25618|FBa *F RERQEQQRRQQQELEEQRQREQEKLQLEKERQLKLEQLFFVQQQEREAHK 750 *F gi|6685149|gb|AAF23815.1|AF216 *F QTRTLELYQEIFQDTLAEICQSEFMSHNRACRSYVSVLDSITRDLVERQM 614 *F CG8919|FBan0008919|CT25618|FBa *F QTRTLELYQEIFQDTLAEICQSEFMSHSRACRSYESMLDSITRDLVERQM 800 *F gi|6685149|gb|AAF23815.1|AF216 *F EQSIYELGVMRVCIRRWRKYRRTQQEKDTLFNQLPLSFGAENPEGVVNKR 664 *F CG8919|FBan0008919|CT25618|FBa *F EQSIYELGVMRVCIRRWRKYRRTQQEKDTLFNQLPLSFGAENPEGVVNKR 850 *F gi|6685149|gb|AAF23815.1|AF216 *F SMEDSLRLSRRYRLGEPCDYGKLLAGLEEHSWLKLDLWHVLDKCLPVAQP 714 *F CG8919|FBan0008919|CT25618|FBa *F SMEDSLRLSRRYRLGEPCDYGKLLAGLEEHSWLKLDLWHVLDKCLPVAQP 900 *F gi|6685149|gb|AAF23815.1|AF216 *F GARRFYKLLISLSGGQEGLQLNCDLDRGLLQQPQSPDARFVEGGYIRGFS 764 *F CG8919|FBan0008919|CT25618|FBa *F GARRFYKLLISLSGGQEGLQLNCDLDRGLLQQPQSPDARFVDGGYIRGFS 950 *F gi|6685149|gb|AAF23815.1|AF216 *F QGIGLSVMKIRDDDHDWKATDLAEANGIICLIGLDDIRLLPDRLKPLLQA 814 *F CG8919|FBan0008919|CT25618|FBa *F QGIGLSVMKIRDDDHDWKATDLAEANGIICLIGLDDIRLLPDRLKPLLQA 1000 *F gi|6685149|gb|AAF23815.1|AF216 *F SRCHDVAVIVQHPANTAFVQPDIPLQKLCLRSFNIFRLRKSGNNRQRLMI 864 *F CG8919|FBan0008919|CT25618|FBa *F SRCHDVAVIVQHPANTAFVQPDIPLQELCLRSFNIFRLRKSGNNRQRLMI 1050 *F gi|6685149|gb|AAF23815.1|AF216 *F ALESAVKFLAKATERKRVGHLHQVETREYLLVNLGSELFRRLKYAAEHDT 914 *F CG8919|FBan0008919|CT25618|FBa *F ALESAVKFLAKATERKRVGHLHQVETREYLLVNLGSELFRRLKYAAEHDT 1100 *F gi|6685149|gb|AAF23815.1|AF216 *F AIRSDTQLNPQRCVDLFNEAVHRLQLVAGEDLSDWPQFPEELRVFVQPLP 964 *F CG8919|FBan0008919|CT25618|FBa *F AIRSDTQLNPQRCVDLFNEAVHRLQLVAGEDLSDWPQFPEELRVFVQPLP 1150 *F gi|6685149|gb|AAF23815.1|AF216 *F IESSLNTNRLEHFEPGWHLPERRQRIVQLLERCKLPKMPVLPRSSSLAEA 1014 *F CG8919|FBan0008919|CT25618|FBa *F IESSLNTNRLEHFEPGWHLPERRQRIVQLLERCKLPKMPVLPRSSSLAEA 1200 *F gi|6685149|gb|AAF23815.1|AF216 *F QCQWVLDYAQISQQEDYVEQIALQAIKILQYDTDDYLNFVEYLAGERMQY 1064 *F CG8919|FBan0008919|CT25618|FBa *F QCQWVLDYAQISQQEDCVEQIALQAIKILQYDTDDYLNFVEYLAGERMQY 1250 *F gi|6685149|gb|AAF23815.1|AF216 *F ILRQERNPPQGVVYNTKTLKRRFLSAWYYEFREPQIYEPVPAEENAQMLE 1114 *F CG8919|FBan0008919|CT25618|FBa *F ILRQERNPPQGIVYNTKTLKRRFLSAWYYEFREPQIYEPVPAEENAQMLE 1300 *F gi|6685149|gb|AAF23815.1|AF216 *F EQPSSQAEVQQLDFDEITSKAEAVLKRFHQRQDERHTLRELNRSHKSRKR 1164 *F CG8919|FBan0008919|CT25618|FBa *F KQPSSQAEVQQLDFDEITSKAEAVLKRFHQRQDERHTLRELNRSHKSRKR 1350 *F gi|6685149|gb|AAF23815.1|AF216 *F RDASDHKHASKHKRKRSKSK------------------------------ 1184 *F CG8919|FBan0008919|CT25618|FBa *F RDASDHKHATLVTLQSTKQLRGDSIHLSKIFVFPFFFLFCFKVFFYIQNH 1400 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TKGFAWNMKSLVAQTECQLRPLKVSSLVSALKAARSDYQGKSSAVCRLVA 1450 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F QMVEADQVKYQWTPNLTSLIDRKDASTRHQWRPHVNHSTRHARKLPVQSI 1500 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F SFLSRCLAKKGEHAGLTSCVLSVKTRYAPKTLHGRELLIWRQSSMRPLLL 1550 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F PSSNLWTGVLSKRHYVPRLRPAEEDEEVERRAVPQLRITKEQSEEEEHQL 1600 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F QRRKDGSRLRNECSEFYLPMGEQDYSEREEENRGHRQAYVPSELMTPELA 1650 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TKWQTTSVTDRDRQLLNLEQQMKTQPSVRMTTQTWFSGNHRRYRSRRSGA 1700 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F KRYHVVSELEGWRRSSNHQPVPVFQGQPGEQEHLRHASHKASARSMPDGQ 1750 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F ERCQVRMPRIRAAKSAVMMAQEARNKHHRLITKKLVYRPRRTVNAVQAEE 1800 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TEDQDTHHRHHGGGQKMSKRAPERALLKQHLADLLAVSKPEDSFRIGYQP 1850 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F NAPTRQLLEQGKCYVSLRREQFIHLHPVRPNSLILAVNLEVKESPRQPVT 1900 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F TLHRRDGAKRPRLVEDVVKPSLITVIRQSAATWDHNGRKVPAKKSNLVGM 1950 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F PSKEHAYQRRLVRNTSTLEAIVKAQAKPRSEPKSPSSATDYHRVASQKLP 2000 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F HVTSKADISKAADPFKDLDKPRIKEKEVASSWKQAYVVRSKMYDAITPYR 2050 *F gi|6685149|gb|AAF23815.1|AF216 *F \-------------------------------------------------- *F CG8919|FBan0008919|CT25618|FBa *F RRAYPGKKCITKDKTDGYFLAKRSSAAAAGSSKKVKPPKQPVVSPKVQVP 2100 *F gi|6685149|gb|AAF23815.1|AF216 \---------------------- *F CG8919|FBan0008919|CT25618|FBa SVLHKKKSRESLGPQTTKTGKL 2122 # *U FBrf0129017 *a Rabouille *b C. *t 2000.5.2 *T personal communication to FlyBase *u FlyBase error report for CG1250 on Tue May 2 04:31:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 02 12:26:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 May 2000 12:26:58 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 2 May 2000 04:31:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: C.rabouille@ed.ac.uk *F Subject: FlyBase error report *F Content-Length: 313 *F Error report from Rabouille (C.rabouille@ed.ac.uk) *F Gene or accession: CG1250 *F Missed gene *F Comments: I have submitted the predicted sequence of Drosophila Sec23A to *F EMBL. The accession number is AJ 276482 *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129018 *a Rabouille *b C. *t 2000.5.2 *T personal communication to FlyBase *u FlyBase error report for CG1422 on Tue May 2 07:21:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 02 15:16:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 May 2000 15:16:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 2 May 2000 07:21:31 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: C.Rabouille@ed.ac.uk *F Subject: FlyBase error report *F Content-Length: 325 *F Error report from RABOUILLE (C.Rabouille@ed.ac.uk) *F Gene or accession: CG1422 *F Missed gene *F Comments: I have submitted the sequence of the cloned cDNA and of the *F predicted protein of dp115 at EMBL under the accession number AJ272048 *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129019 *a Elphick *b M.R. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG10738 on Wed May 3 09:36:25 2000. *F From m.r.elphick@qmw.ac.uk Wed May 03 09:28:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 09:28:54 \+0100 *F X-Sender: ugbt136@alpha.qmw.ac.uk *F Mime-Version: 1.0 *F Date: Wed, 3 May 2000 09:36:25 \+0100 *F To: flybase-help@morgan.harvard.edu *F From: Maurice Elphick *F Subject: CG10738 *F I have performed a BLAST search for the putative protein product of the *F Drosophila CG10738 gene and it appears that there is an error in the *F Flybase report for this gene: *F 'D. melanogaster gene CG10738 is reported here . It encodes a product with *F the function G protein linked receptor involved in DNA replication which is *F a component of the cell , the centrosome , the *F cytoplasm , the cytosol , the intracellular , the nucleus , the plasma *F membrane , the ribosome and the unlocalised' *F BLAST analysis shows that the CG10738 displays no sequence similarity with *F G protein linked receptors. Rather it is most closely related to the family *F of receptor guanylyl cyclases exemplified by *F 1. the membrane-associated receptors expressed in sea urchin sperm cells *F that are activated by egg-derived peptides. *F and *F 2. mammalian atrial natriuretic peptide receptors. *F Dr. Maurice R. Elphick *F Senior Lecturer in Animal Physiology and Neuroscience *F School of Biological Sciences *F Queen Mary and Westfield College *F University of London *F London E1 4NS, UK *F Tel: \+44(0) 20 7882 5290 *F Fax: \+44(0) 20 8983 0973 *F E-mail: M.R.Elphick@qmw.ac.uk # *U FBrf0129020 *a Whitfield *b E. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG18009 on Wed May 3 03:42:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 11:37:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 11:37:23 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 03:42:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 7210 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18009 *F Gene annotation error *F Gene CG18009 corresponds to FBgn0026758; Trf2 *F Comments: Examination of CDS feature and alignment of translation of: *F AE003444; AAF46368.1 (Trf2, SPTREMBL:Q9W3F9) *F and *F AE003444; AAF46369.1 (CG18009, SPTREMBL:Q9W3F8) *F demonstrates these 2 genes are indeed the same, just different *F splice versions. *F thanks *F clustal below *F CLUSTAL W (1.8) multiple sequence alignment *F Q9W3F9 MGSAVKKGDGSPTRILEEYQVARDLRNEDLFPEHPVEHPDEDSDKENQSPNQSPNPRAIG *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 DCEQGPDIGKDTLNPNEADQFSSEEVEKNEANSSCNESGGDARNSRDRLSSSHSEDEQLN *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 QEYSTSSSGSTIGNREQGSDVGKDTSSRNEADQFSNEEVEKNEANSSGNESYGDACNSSD *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 RLSSSNSEDEQLHQEYSTSSRGRTREYAQSDLNPGYQDLNIGNQDLNQVNREYQESNNEY *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 QVSNQEYQHLNTEYQELNHGDQDSNDEYQELNHGNQDSNDEYQESNHGNQDSNDEYQELN *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 HSNQDSNDEYQELNHGDQDSSDEYQDLNHVNQDSNHEYQAWNHEYQDSNHEYQALNREYQ *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 YPESDEEEAARKRNIYTRLRSYACQVHLPRLGESEVDSDDRRHNPINQPFNQPINRPNQK *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 SDSSLESSSPERQDSESESRQASDEESSSGDSDYLIDPVTGWLSRRRETSTEREEKSDQS *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 QQESSEEITDEQKEESDDVEQQSPAEHTSSDRRDHSPDKQQNKRKSLGTGNSSYSKKPLL *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 DGDKGDPRSHLSSLGSRRSLKRKWPFDCTDSKKFKPNLQSEEDKSEEEKREDTESECSDE *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 EDSDSSSFDTDSNSDSDSNLDLDSDSDSHSDFESSSSSEESAQDPAPEGETDQDPAPEGE *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 ADQDPAPEGGVDQDPAPEGGVDQDPAPEGEVDQDPAPEGESDQDLAPEGGVDQDPAPEFG *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 TDQDPHPYYNDQDPAPYYDDQDPASYYDDHICDSEEDYKPFIFSKERKFLKFVTNPLGDD *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 KSSLDLSIQLYKVFGAYKLEDVYSSIDEEGQPEQDLQLKQQQKHLKKYQKLQLVVFWHQE *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 KFFSKEQLLLYEYLKLLLQKFFPQEQQEKRLQQEKLLQQEKLLQQEKRLQQEKRLQQEKR *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 LQQEKRLQQEKRLQQQKLLQQQKLLQQEKLLQQQKLLQQEKLLQQEKLLQQEKQLQQEKH *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 LPQERQLQQDKVFLQLRQLQQKKELEEQRERKRHLFEQQKQFVLEKLRQQKEQHRRQQNR *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 VKRERKLRQKKEKQRLEYERFLKEYREDCKRQLRLQREHYLIKIKQLEKASQRSLKGNRK *F Q9W3F8 \------------------------------------------------------------ *F Q9W3F9 HKLMQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTN *F Q9W3F8 \---MQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTN *F Q9W3F9 RQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLA *F Q9W3F8 RQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLA *F Q9W3F9 TGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDE *F Q9W3F8 TGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDE *F Q9W3F9 EESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGM *F Q9W3F8 EESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGM *F Q9W3F9 VTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL *F Q9W3F8 VTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVL *F Q9W3F9 GTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASV *F Q9W3F8 GTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASV *F Q9W3F9 NHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNI *F Q9W3F8 NHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNI *F Q9W3F9 FVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEK *F Q9W3F8 FVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEK *F Q9W3F9 ANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINA *F Q9W3F8 ANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINA *F Q9W3F9 ASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQ *F Q9W3F8 ASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQ *F Q9W3F9 QQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF *F Q9W3F8 QQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129021 *a Whitfield *b E. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG7061 on Wed May 3 06:28:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 14:23:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 14:23:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 06:28:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 672 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7061 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene BcDNA:LD23336 *F = CG7061. *F Comments: The BcDNA:LD23336 cDNA isolated by Rubin et al, 1999 (AF145682; *F AAD38657.1), corresponds to a probable third isoform not annotated in *F AE003634. *F Two isoforms are currently annotated, AAF53156.1 and AAF53157.1, *F and translation of sequence reveals the DNA is present to *F translate the third isoform (I did not work out coordinates, sorry) *F that matches 100% with the cDNA. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129022 *a Whitfield *b E. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG7945 on Wed May 3 06:00:33 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 13:55:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 13:55:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 06:00:33 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 706 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7945 *F Gene annotation error *F Genes CG7945 and CG17014 should be merged. *F Comments: Looking at CDS features and alignment of: *F AE003530; AAF49623.1 (CG17014) (longest sequence of 1310aa) *F AE003530; AAF49625.1 (CG7945) *F AE003530; AAF49626.1 (CG7945) *F it is obvious all three are splice variants of the same gene *F Comparison to AAF49623, longest isoform: *F AAF49625 is missing the first 1062 amino acids then aligns 100% *F for the remaining amino acids *F AAF49626 has a unique N-terminal exon, then aligns 100% for *F the remaining 181 amino acids *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129023 *a Whitfield *b E. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG1484 on Wed May 3 07:24:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 15:20:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 15:20:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 07:24:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 838 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1484 *F Gene annotation error *F Gene CG1484 has incorrect exon/intron structure. *F Comments: I believe there may be a sequence error or splicing error that *F terminates fliI protein at 1066 amino acids in the Celera sequence *F (AE003568; AAF50830.1). *F 3 other groups have sequenced fliI and isolated a 1256 amino acid *F protein, see TrEMBL Q24020. One of these groups is Rubin et al, 1999, *F submitting a sequence of a full length cDNA from the same strain *F (AF132184; AAD34772.1). *F Translating downstream sequences I found residues 1069-1256 that aligned *F perfectly with the current sequence. I failed to find residues 1067-1068 *F which is why I suspect a sequencing error. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129024 *a Whitfield *b E. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG3647 on Wed May 3 08:32:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 16:28:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 16:28:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 08:32:50 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 567 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3647 *F Gene annotation error *F Gene stc has incorrect exon/intron structure. *F Comments: Translation of stc starts at an earlier Met than that specified in the *F Celera CDS feature of AE003646; AAF53441.1. There is an upstream Met *F in the same frame 23 codons away. Use of this Met as the translation *F start is confirmed by the Berkeley cDNA submitted by Rubin et al, 1999, *F in AF145679; AAD38654.1 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129025 *a Wojtas *b K. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG1379 on Wed May 3 10:48:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 18:44:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 18:44:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 10:48:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kwojtas@life.bio.sunysb.edu *F Subject: FlyBase error report *F Content-Length: 469 *F Error report from Kathy Wojtas (kwojtas@life.bio.sunysb.edu) *F Gene or accession: AE003571 *F Gene annotation error *F Genes CG1379 and CG15453 should be merged. *F Comments: CG1379 contains a Runt domain, which should be followed at some *F point by a VWRPY, if this Runt domain protein is like all other known Runt *F domain proteins. The VWRPY is contained in the predicted gene CG15453. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129026 *a Wojtas *b K. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG15455 on Wed May 3 18:47:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 18:47:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 18:47:45 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 10:52:33 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kwojtas@life.bio.sunysb.edu *F Subject: FlyBase error report *F Content-Length: 431 *F Error report from Kathy Wojtas (kwojtas@life.bio.sunysb.edu) *F Gene or accession: AE003571 *F Gene annotation error *F Genes CG15455 and CG15454 should be merged. *F Comments: CG15455 contains a Runt domain, which should be followed at some *F point by a VWRPY, if this Runt domain gene is like all known Runt domain *F genes. CG15454 contains a VWRPY. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129027 *a Wojtas *b K. *t 2000.5.3 *T personal communication to FlyBase *u FlyBase error report for CG1379 on Wed May 3 11:00:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 03 18:55:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 3 May 2000 18:55:36 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 3 May 2000 11:00:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kwojtas@life.bio.sunysb.edu *F Subject: FlyBase error report *F Content-Length: 411 *F Error report from Kathy Wojtas (kwojtas@life.bio.sunysb.edu) *F Gene or accession: CG1379 *F Gene annotation error *F Gene CG1379 has incorrect exon/intron structure. *F Comments: RT-PCR evidence indicates that exon 4 of this gene is incorrect, and *F the correct exon 4 would begin at approximately nucleotide 19210 of the contig. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129028 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG7740 on Thu May 4 01:52:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 09:47:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 09:47:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 01:52:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 526 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7740 *F Gene annotation error *F Gene prominin-like has incorrect exon/intron structure. *F Comments: Previously submitted cDNA of prominin-like (AF127935; AAD22487.2 and *F AF197345; AAF07212.1) shows that the init Met defined in Celera *F sequence AE003477; AAF47716.1 is incorrect. The correct Met is the one *F encoded 18 amino acids upstream in the same frame. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 10:06:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 10:06:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 02:11:23 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 705 *F . *F . *F . *F PS I also checked for the previous update I sent for prominin-like and the *F codons are there too for the upstream 18 amino acid, I forgot to mention *F that \- sorry! # *U FBrf0129029 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG11992 on Thu May 4 02:11:23 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 10:06:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 10:06:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 02:11:23 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 705 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11992 *F Gene annotation error *F Gene Rel has incorrect exon/intron structure. *F Comments: Previously submitted cDNA of Rel (U62005; AAB17264.1) shows that the *F init *F Met defined in Celera sequence AE003681; AAF54333.1 is incorrect. The *F correct Met is the one encoded 2 amino acids upstream in the same frame. *F I have checked the translation and the codons are present. *F thanks *F . *F . *F . *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129030 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG17822 on Thu May 4 02:22:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 10:17:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 10:17:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 02:22:31 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 797 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17822 *F Gene annotation error *F Genes CG17822 and CG18476 should be merged. *F Comments: Looking at the CDS features for: *F CG17822 *F AE003692; AAF54676.1 *F FT CDS join(complement(153176..153523), *F FT complement(152536..153117),complement(151688..152464)) *F CG18476 *F AE003692; AAF54675.1 *F FT CDS join(complement(154618..154749), *F FT complement(154138..154550),complement(153176..154082), *F FT complement(152536..153117),complement(151688..152464)) *F it is obvious these 2 genes are, in fact, alternatively spliced isoforms of *F the same gene \- please merge them. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129031 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG4376 on Thu May 4 02:42:06 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 10:37:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 10:37:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 02:42:06 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 666 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4376 *F Gene annotation error *F Gene Actn has incorrect exon/intron structure. *F Comments: On this occassion there is no supporting evidence of a cDNA but surely *F the initiating Met should be the first one encoded in frame downstream *F of a stop codon within an exon? *F CDS features for Actn (2 spliced isoforms): *F AE003422; AAF45705.1 *F AE003422; AAF45706.1 *F should adjusted to encode the first Met in frame just 2 codons *F upstream. This update will adjust the sequence to match that *F submitted by EDGP. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129032 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG12548 on Thu May 4 03:01:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 10:56:25 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 10:56:25 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 03:01:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 722 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12548 *F Gene annotation error *F Genes CG12548 and CG14465 should be merged. *F Comments: CDS features for: *F CG12548 *F AE003781; AAF57216.1 *F FT CDS join(complement(32793..33192),complement(30136..30164), *F FT complement(29305..30046),complement(24480..24679), *F FT complement(21594..22485),complement(21319..21392), *F FT complement(20068..20412)) *F CG14465 *F AE003781; AAF57217.1 *F FT CDS complement(20068..20397) *F suggest these genes are in fact different isoforms of the same protein. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129033 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG17771 on Thu May 4 03:58:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 11:54:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 11:54:27 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 03:58:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 686 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17771 *F DNA error at position 114547. Upstream nucleotides: aacagtgatttctgctccgt *F Insertion of length 1: G. *F Corrected sequence: GGCTGCAGGCACGGAGCAGA. *F Comments: The sequence error reported is based on comparison to a submitted *F cDNA, U32626; AAA75448.1. *F The cDNA does not have the base 'G' as shown above and consequently *F alters the C terminal sequence and reads through to an earlier *F termination codon. *F Could you please check the Celera genomic sequence at this region to *F confirm if the cDNA is correct or not. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From eleanor@ebi.ac.uk Thu May 04 12:02:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 12:02:20 \+0100 *F Date: Thu, 04 May 2000 12:04:26 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: FlyBase-error@hedgehog.lbl.gov *F CC: flybase-updates@morgan.harvard.edu, Eleanor Whitfield *F Subject: Re: FlyBase error report \- CG17771 *F Content-Transfer-Encoding: 7bit *F Hi All, *F I have just noticed an error in the FlyBase error report I just set for *F CG17771 *F The CDS for CG17771 is reading the complement so I got a little confused! *F what I would like to replace is this line: *F Corrected sequence: GGCTGCAGGCACGGAGCAGA. *F with this line: *F Corrected sequence: ctccgtgcc *F then everything should be OK *F thanks *F Nellie # *U FBrf0129034 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG17771 on Thu May 4 04:25:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 12:20:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 12:20:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 04:25:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 864 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17771 *F Gene annotation error *F Genes CG17771 and CG17772 should be merged. *F Comments: Irrespective of the possible sequencing error I have reported for this *F gene, the CDS features suggest that these 2 genes are in fact the same: *F CG17771 *F AE003474; AAF47626.1 *F FT CDS complement(113452..115119) *F CG17772 *F AE003474; AAF47625.1 *F FT CDS join(complement(115838..116528), *F FT complement(115576..115736),complement(113452..115230)) *F alternative splicing again! *F The possible sequencing error is in the common exon. *F thanks *F I wasn't sure if I should only report one error at a time for each gene? *F But then I hope I do not find many cases of a frameshift and splicing! *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129035 *a Spring *b J. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG10497 on Thu May 4 05:27:21 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 13:22:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 13:22:37 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 05:27:21 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: J.Spring@unibas.ch *F Subject: FlyBase error report *F Content-Length: 1732 *F Error report from J. Spring (J.Spring@unibas.ch) *F Gene or accession: CG10497 *F Gene annotation error *F Gene CG10497 has incorrect exon/intron structure. *F Comments: /gene='Sdc' AE003454 CT27296 CG10497 AAF46724 gi:7291294 *F The 85 aa Sdc gene product (gi:7291294) does not make sense. With few splice *F changes the genomic data *F can be adjusted to the published full length cDNA sequence and to 15 ests, so *F that a 399 aa protein *F product that is similar to other syndecan (Sdc) family members. The chages are *F marked with asterisks; *F the first (23078 instead of 23080) leads to a frame shift, the second is in *F the 5'untranslated and *F the last corresponds to the 5' end of the longest cDNA clone (U03282) and do *F not affect the protein. *F The new product of 299 aa still differs at 3 places from the published protein *F of 295 aa (sw:sdc_drome). *F But these changes are in repetitive regions and could be polymorphisms. Only *F the short cytoplasmic *F domain of syndecans is highly conserved (which is now missing in gi:7291294). *F Antibodies raised *F against recombinant protein also confirmed a larger protein than 85 aa (Spring *F et al. 1994). *F In a mRNA spliced according to the following corrected numbers: *F mRNA *F complement(join(4890..5502,5645..5812,8832..9350,9942..10080,230*78*..23185,908 *F 59..911*24*,91782..919*36*)) *F the coding sequence would be: *F CDS complement(join(5330..5502,5645..5812,8832..9350,9942..10080,23078..23185,9 *F 0859..90951)) *F Reference: *F Spring J, Paine-Saunders SE, Hynes RO, Bernfield M. *F Drosophila syndecan: conservation of a cell-surface heparan sulfate *F proteoglycan. *F Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3334-8. *F Yours sincerely, *F J. Spring *F Browser: Mozilla/4.5 en (WinNT; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129036 *a Leptin *b M. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG5408 on Thu May 4 08:10:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 16:06:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 16:06:01 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 08:10:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mleptin@uni-koeln.de *F Subject: FlyBase error report *F Content-Length: 274 *F Error report from Maria Leptin (mleptin@uni-koeln.de) *F Gene or accession: CG5408 *F Gene annotation error *F Gene CG5408 corresponds to AF204688 (FBgn0028978) *F Comments: sequence identity *F Browser: Mozilla/4.6 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129037 *a Taghert *b P. *t 2000.5.2 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue May 02 13:12:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 May 2000 13:12:01 \+0100 *F To: taghertp@thalamus.wustl.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 2 May 2000 13:17:00 \+0100 *F Content-Length: 1022 *F Dear Dr. Taghert, *F I am curating your abstract for FlyBase: *F Hwang et al., 1999, Molec. Biol. Cell 10(Suppl.): 108a *F 'Prohormone convertase 2 and 7B2 from Drosophila melanogaster: Insect *F cell-specific expression of activity.' *F In this you mention a gene that is new to FlyBase: 'd7B2'. *F I would be grateful if you could tell me the EST ID number (e.g. *F HL03564, LD73454) that corresponds to this gene. *F Also, do you have a map location for it or do you know which 'CG' in *F FlyBase it corresponds to ? It is nice if we can keep as many gene *F records as possible anchored to the genome. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From taghertp@pcg.wustl.edu Tue May 02 15:03:10 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 May 2000 15:03:10 \+0100 *F Date: Tue, 2 May 2000 09:10:44 \-0500 (CDT) *F Mime-Version: 1.0 *F To: Gillian Millburn (Genetics) *F From: Paul Taghert *F Subject: Re: FlyBase query *F Dr. Milburn: *F Thank you for your message. The d7B2 sequence corresponds to *F CG1168 *F GH01053.5 *F Please let me know if you would like more information. *F sincerely, *F Paul Taghert *F Paul Taghert *F taghertp@thalamus.wustl.edu *F tel. 314 362-3641 *F fax 314 362-3446 *F Box 8108 *F Anatomy & Neurobiology *F Washington University Medical School *F 660 South Euclid Avenue *F Saint Louis, MO 63110 # *U FBrf0129038 *a Crane-Robinson *b C. *t 2000.5.7 *T personal communication to FlyBase *u FlyBase error report for CG12098 on Sun May 7 20:58:39 2000. *F From cranerobinsonc@hotmail.com Sun May 07 20:58:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 7 May 2000 20:58:39 \+0100 *F X-Originating-IP: 156.40.160.136 *F From: 'Colyn Crane-Robinson' *F To: flybase-help@morgan.harvard.edu *F Subject: CG12098 *F Date: Sun, 07 May 2000 21:02:44 BST *F Mime-Version: 1.0 *F CG12098 is indeed a sequence specific HMG box protein (in fact a Sox *F protein, and the frame is clearly correct). But the Met given as the *F initiation point (MNAF----) cannot be correct since it is located at a key *F point in the N-terminal extended segment of the HMG box, without which the *F HMG box would not fold properly. So the initiating Met must be earlier. *F You could find an in-frame one better than I! *F (I've been HMG boxing for years, which is why I spotted it). *F CC-R *F Prof. Colyn Crane-Robinson, *F Biophysics Laboratories, *F St. Michael's Building, *F University of Portsmouth, *F Portsmouth, PO1 2DT, UK. *F Tel: (44)-23-92842055 *F Fax: (44)-23-92842053 *F e-mail: colyn.crane-robinson@port.ac.uk or *F cranerobinsonc@hotmail.com # *U FBrf0129039 *a Crane-Robinson *b C. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG6419 on Tue May 9 19:50:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 19:50:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 19:50:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 11:55:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: colyn.crane-robinson@port.ac.uk *F Subject: FlyBase error report for CG6419 on Tue May 9 11:55:27 2000 *F Content-Length: 487 *F Error report from Colyn Crane-Robinson (colyn.crane-robinson@port.ac.uk) *F Gene or accession: CG6419 *F Missed gene *F Comments: The translation in FlyBase ends \-SKRLE but this is within the *F Sox-type HMG box and so it cannot be the finish. It is certain that the *F sequence continues: XXWX- where X I will not guess at but the W is as solid as *F a rock. This may help you decide the splicing etc. *F Browser: Mozilla/4.04 (Macintosh; I; PPC, Nav) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129040 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG12414 on Mon May 8 02:33:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:29:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:29:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:33:50 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 707 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12414 *F Gene annotation error *F Genes CG12414 and CG17552 should be merged. *F Comments: CDS feature for above genes show they share the same C terminal exons *F so should be merged and annotated as splice variants. *F CG12414 *F AE002665; AAF45409.1 *F FT CDS join(<7917..7918,14769..14886,31147..31256,42546..42730, *F FT 50635..50843,66364..66479,67455..67623,67690..67826, *F FT 67877..68020,82008..82182,87453..87650) *F CG17552 *F AE002665; AAF45408.1 *F FT CDS join(82057..82182,87453..87650) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129041 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG7446 on Mon May 8 02:36:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:32:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:32:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:36:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 767 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7446 *F Gene annotation error *F Gene Grd has incorrect exon/intron structure. *F Comments: Translation needs to be extended by 3 amino acids *F FT CDS join(complement(238796..239174), *F FT complement(237308..237375),complement(236635..236938), *F FT complement(230854..231219),complement(229820..230754)) *F amend first exon: *F complement(238796..239174) *F > *F complement(238796..239183) *F to extend translation to first Met. *F This sequence update is confirmed by cDNA isolated by Harvey et al, J. *F Neurochem. 62:2480-2483(1994), sequence accession: X78349; CAA55144.1 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129042 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG4199 on Mon May 8 02:38:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:34:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:34:09 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:38:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 572 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4199 *F Gene annotation error *F Gene EG:22E5.5 has incorrect exon/intron structure. *F Comments: Comparison to EDGP sequence (AL031765; CAA21128.1) reveals the *F Celera translation *F is 113 amino acids too short at the N terminus. I have translated the DNA and *F found *F the missing 2 exons that match exactly to the EDGP sequence (no coordinates, *F sorry). *F Please update the CDS feature of AE003423; AAF45717.1. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129043 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG3539 on Mon May 02:39:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:35:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:35:01 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:39:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 634 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3539 *F Gene annotation error *F Gene Slh has incorrect exon/intron structure. *F Comments: Genomic sequence from Merli et al, Genes Dev. 10:1260-1270(1996), *F sequence: *F U63852; AAC47550.1 identifies a possible upstream init_met that is present in *F the *F Celera sequence with 100% identity. *F please amend present CDS feature: *F FT CDS join(42341..42486,42544..43226,43303..44399) *F > *F FT CDS join(42225..42252,42327..42486,42544..43226,43303..44399) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129044 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG10328 on Mon May 8 10:40:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:40:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:40:54 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:44:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 888 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10328 *F Gene annotation error *F Gene nonA-l has incorrect exon/intron structure. *F Comments: nonA-l has been sequenced as part of the bithorax complex gene *F region, Martin *F et al, Proc. Natl. Acad. Sci. U.S.A. 92:8398-8402(1995), sequence U31961; *F AAA84417.1. *F This sequence has been annotated as a fragment but examination of the *F translation *F reveals that the codon immediately prior to the first amino acid of the protein *F fragment is a stop codon. Celera sequence begins at the first Met after the *F stop codon *F (13 amino acids downstream). Could you please examine the region again and *F decide if *F there are any exons further upstream as suggested by Martin et al, or if the *F Met you *F annotated is indeed the initiating Met. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129045 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG12598 on Mon May 8 02:43:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:41:51 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:41:51 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:43:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 576 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12598 *F Gene annotation error *F Gene EG:BACN35H14.1 has incorrect exon/intron structure. *F Comments: Comparison to EDGP sequence (AL035207; CAA22774.1) reveals the *F Celera translation *F is 21 amino acids too short at the N terminus. I have translated the DNA and *F found *F the missing exon that matches exactly to the EDGP sequence (no coordinates, *F sorry). *F Please update the CDS feature of AE003422; AAF45665.1. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129046 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG9390 on Mon May 8 02:51:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 10:47:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 10:47:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 02:51:54 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 865 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9390 *F DNA error at position 8017. Upstream nucleotides: ttgccctccctgaaacaatg *F Substitution: base T should be C. *F Comments: The current translation for AE003594; AAF51696.1 terminates after 10 *F amino acids. This *F isoform of AcCoAS has also been sequenced by Russell et al, submitted 1994, *F sequence *F Z46786; CAA86738.1. This sequence continues for another 571 amino acids to *F generate the *F full length isoform. The C terminal 576 aa of the sequence from Russell *F aligns with the *F second isoform annotated by Celera AE003594; AAF51695. *F I believe the different isoforms differ only at the N terminus due to *F alternative exon *F splicing, not due to premature termination of the sequence after 10 amino acids. *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129047 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG18414 on Mon May 8 04:11:13 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 12:08:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 12:08:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 04:11:13 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 1091 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18414 *F Gene annotation error *F Gene CG18414 has incorrect exon/intron structure. *F Comments: CG18414 aligns perfectly with the C-terminus of ph-p, as sequenced *F by EDGP (Z98269; *F CAB10975.1). The N terminus of ph-p seems to be defined by Celera in AE003423; *F AAF45726.1. *F CG18414 must be merged with ph-p and I think the CDS of Celera CG18414 and *F Celera ph-p *F should be merged to generate the full length ph-p. *F One concern is that the GadFly sequence entry for ph-p and the jam file for *F ph-p are of a *F different lengths and between them there are some sequence differences. Also, *F the *F match to a known ph-p cDNA (X63672; CAA45211.1) is poor in regions. *F The CDS for Celera ph-p has a note to say transcript structure is suspect *F (AE003423; *F AAF45726.1), I would have to agree and was hoping it could be cleared up in the *F reannotation. Also, intriguingly, ph-d appears to be missing from Celera *F annotation! *F Is it there? *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129048 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG9842 on Mon May 8 04:24:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 12:20:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 12:20:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 04:24:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 1885 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9842 *F Gene annotation error *F Gene CG9842 corresponds to FBgn0005783 *F Comments: Currently CG9842 is under Pp12-14D, but I believe it should be under *F PpD33. *F Celera sequence of CG9942 AE003502; AAF48622.1 aligns 100% with genomic *F sequence of *F PpD33 S40008; AAB22469.1 *F d33_xxxxx \--------------------------------------------------------CCAG *F cele_xxxxx GAGAAGACGATGATCGATATTGAGGCGCCGGTGACGGTGTGCGGTGATATCCACGGCCAG *F d33_xxxxx TTCTACGACCTGATGAAGCTGTTCGAAGTGGGCGGCTCGCCCGCGAGCACCAAGTATCTG *F cele_xxxxx TTCTACGACCTGATGAAGCTGTTCGAAGTGGGCGGCTCGCCCGCGAGCACCAAGTATCTG *F d33_xxxxx TTCCTGGGC--------------------------------------------------- *F cele_xxxxx TTCCTGGGCGACTACGTCGATCGGGGCTACTTCAGCATCGAGTGCGTCCTGTATTTGTGG *F the alignment to Pp12-14D is not as good: *F 14d_xxxxx \--------------------------------------------------------CCAG *F cele_xxxxx GAGAAGACGATGATCGATATTGAGGCGCCGGTGACGGTGTGCGGTGATATCCACGGCCAG *F 14d_xxxxx TTCTACGATCTGATGAAGCTATTCGAGATTGGGNNCTCGCCGGCGACCACCAAGTATCTG *F cele_xxxxx TTCTACGACCTGATGAAGCTGTTCGAAGTGGGCGGCTCGCCCGCGAGCACCAAGTATCTG *F 14d_xxxxx TTCCTGGNN--------------------------------------------------- *F cele_xxxxx TTCCTGGGCGACTACGTCGATCGGGGCTACTTCAGCATCGAGTGCGTCCTGTATTTGTGG *F Maybe other evidence was used to asign CG9842 to Pp12-14D but based on *F sequence I think *F it should be under PpD33. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129049 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG8402 on Mon May 8 05:06:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 13:03:19 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 13:03:19 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 05:06:46 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 450 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8402 *F Gene annotation error *F Genes CG8402 and PpD3; FBgn0005777 should be merged. *F Comments: Celera sequence for CG8492: *F AE003684; AAF54438.1 *F and genomic DNA for PpD3: *F S39960; AAB22463.1; *F align 100%, amino acids 268-292 of Celera sequence match the short *F published fragment. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129050 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG2699 on Mon May 8 05:33:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 13:51:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 13:51:45 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 05:33:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 725 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2699 *F DNA error at position 7676. Upstream nucleotides: gtggagcggggagggttgca *F Substitution: base C should be T. *F Comments: Celera sequence: *F AE003590; AAF51510.1 *F encodes a 496 aa protein. *F Published cDNAs, Weinkove et al, J. Biol. Chem. 272:14606-14610(1997): *F Y12498; CAA73100.1 *F and *F Y11143; CAA72030.1 *F have a 506 amino acid protein that extends 10 amino acids upstream from *F the Celera N-terminus. *F Could you please check the sequence as stated above to see if the Celera *F sequence really does have an upstream initiating Met that agrees with *F the cDNAs. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129051 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG5498 on Mon May 8 06:22:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 14:25:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 14:25:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 06:22:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 764 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5498 *F Gene annotation error *F Gene BcDNA:GH08385 has incorrect exon/intron structure. *F Comments: CDS feature for BcDNA:GH08385, AE003591; AAF51586.1 *F FT CDS join(complement(52956..53348),complement(52684..52903), *F FT complement(52413..52622),complement(51898..52343)) *F encodes for a protein 26 amino acids shorter than the cDNA sequenced *F from Berkeley, Rubin et al, 1999, AF145648; AAD38623.1. *F The DNA for this upstream 26 amino acids is present in the Celera *F sequence, pease amend the first coding exon: *F complement(52956..53348) *F becomes *F complement(52956..53426) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129052 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG6404 on Mon May 8 06:34:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 14:46:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 14:46:46 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 06:34:53 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 650 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6404 *F Gene annotation error *F Gene BcDNA:GH02220 has incorrect exon/intron structure. *F Comments: CDS feature for BcDNA:GH02220, AE003547; AAF50127.1 *F FT CDS join(2261..2299,2411..3467,3542..3726) *F encodes for a protein 15 amino acids shorter than the cDNA sequenced *F from Berkeley, Rubin et al, 1999, AF145596; AAD38571.1. *F The DNA for this upstream 15 amino acids is present in the Celera *F sequence, please amend the first coding exon: *F 2261..2299 *F becomes *F 2216..2299 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129053 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG4995 on Mon May 8 07:18:04 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 15:14:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 15:14:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 07:18:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 532 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4995 *F Gene annotation error *F Genes CG4995 and CG18626 should be merged. *F Comments: CDS features for CG4995 and CG18626 suggest the genes are different *F isoforms of the same gene, not separate genes. *F CG4995 *F FT CDS join(85877..85918,86755..86863,86932..87393,87463..88049) *F CG18626 *F FT CDS join(86830..86863,86932..87393,87463..88049) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129054 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG12223 on Mon May 8 08:24:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 16:20:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 16:20:09 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 08:24:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 748 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12223 *F Gene annotation error *F Gene bss has incorrect exon/intron structure. *F Comments: Celera CDS feature of bss (AE003502; AAF48594.1): *F FT CDS join(3631..3936,4044..4202,4279..4442,4574..4748, *F FT 4818..5000) *F should be extended to encode an upstream possible initiating Met to generate a *F longer protein. *F FT join(1583..1606,3460..3936,4044..4202,4279..4442, *F FT 4574..4748,4818..5815) *F this sequence will then perfectly match a genomic sequence X89811; *F CAA61938.1 from Canaple et al, Gene 184:285-290(1997). *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129055 *a Waggener *b J.M. *c P.J. *d DiMario *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG7421 on Tue May 9 12:48:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 20:43:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 20:43:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 12:48:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: pdimari@unix1.sncc.lsu.edu *F Subject: FlyBase error report for AF162774 or CG7421 on Tue May 9 12:48:39 2000 *F Content-Length: 918 *F Error report from John M. Waggener and Patrick J. DiMario *F (pdimari@unix1.sncc.lsu.edu) *F Gene or accession: AF162774 or CG7421 *F Missed gene *F Comments: Our cDNA sequence (acc. no. AF162774) corresponds to CT22845 from *F CG7421. The deduced Drosophila protein is closely related to vertebrate *F nucleo-phosphoprotein 140 (Nopp140) throughout most of its length. The *F Drosophila protein differs significantly from vertebrate Nopp140 in that it *F contains a prominent glycine, arginine, and phenylalanine rich domain near its *F carboxy tail. This tail domain is similar to the one found in vertebrate *F nucleolin proteins. When expressed as a GFP fusion, the Drosophila protein *F localizes to nucleoli, but not to Cajal (coiled) bodies. Please see Waggener *F and DiMario (1999) Mol. Biol. Cell (Suppl.) Vol. 10, pg 439a. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129056 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG11767 on Tue May 9 01:22:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 09:17:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 09:17:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 01:22:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11767 on Tue May 9 01:22:17 2000 *F Content-Length: 859 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11767 *F Gene annotation error *F Genes CG11767 and Or24D should be merged. *F Comments: Or24D is identified in Clyne et al, Neuron 22:327-338(1999). In the *F paper they *F provide the CDS base span for Or24D in AC004371 BDGP sequence entry. *F Also the base span they provide starts from an upstream Met that is in frame *F with the initiating Met defined by Celera AE003577; AAF51040.1. *F Please amend the CDS feature: *F FT CDS join(145574..145855,145910..146061,146143..146515, *F FT 146568..146666,146722..146874,146936..146986) *F to: *F FT CDS join(145541..145855,145910..146061,146143..146515, *F FT 146568..146666,146722..146874,146936..146986) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From eleanor@ebi.ac.uk Tue May 09 09:23:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 09:23:42 \+0100 *F Date: Tue, 09 May 2000 09:27:15 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: Re: FlyBase error report for CG11767 on Tue May 9 01:22:17 2000 *F Content-Transfer-Encoding: 7bit *F Apologies, I have just noticed a typo in my last message: *F Genes CG11767 and Or24D should be merged. *F should read: *F Genes CG11767 and Or24D.1 should be merged. *F For all mentions of Or24D please read Or24D.1. *F Sorry about that! *F thanks *F Nellie # *U FBrf0129057 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG8766 on Tue May 9 02:45:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 10:41:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 10:41:26 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 02:45:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG8766 on Tue May 9 02:45:22 2000 *F Content-Length: 783 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8766 *F DNA error at position 61375. Upstream nucleotides: tggatgtgcccaccggcgtg *F Insertion of length 1: g. *F Corrected sequence: GCGTGCGATG. *F Comments: FBrf0113255 == Benevolenskaya, 1997.8.7, GenBank/EMBL/DDBJ: AF017783 *F Sequence from this Ref is a cDNA that corresponds to the shorter isoform *F annotated by Celera AE003821; AAF58462.1 *F Sequence comparison reveals the cDNA has a, with respect to Celera, *F deletion of one base. This deletion causes a frameshift that identifies a *F different possible initiating Met for the shorter splice isoform. *F Could you please check the sequence to confirm which is correct. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129058 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG9983 on Tue May 9 03:00:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 10:56:17 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 10:56:17 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 03:00:42 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9983 on Tue May 9 03:00:42 2000 *F Content-Length: 1022 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9983 *F Gene annotation error *F Gene Hrb98DE has incorrect exon/intron structure. *F Comments: CDS feature for Hrb98DE *F AE003766; AAF56800.1 *F FT CDS join(30986..31033,31740..32229,33806..33981,34046..34352, *F FT 34642..34711,35095) *F position 34711 does not conform to the consensus splice site for the *F beginning of an intron. If you read through the 'splice site' at 34711 the *F protein encodes one further C terminal amino acid then reads a stop codon. *F please amend the CDS feature to read: *F FT CDS join(30986..31033,31740..32229,33806..33981,34046..34352, *F FT 34642..34715) *F This translated protein then agrees with M25545; AAA28622.1 from Haynes *F et al, Mol. Cell. Biol. 10:316-323(1990) *F The mRNA feature also needs to be amended to provide the correct splice site for *F the exon/intron boundary. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129059 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG13358 on Tue May 9 03:32:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 11:28:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 11:28:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 03:32:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG13358 on Tue May 9 03:32:14 2000 *F Content-Length: 550 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG13358 *F Gene annotation error *F Gene EG:34F3.10 has incorrect exon/intron structure. *F Comments: EG:34F3.10 was isolated by EDGP: *F AL031583; CAB41346.1 *F They define a further 2 upstream exons so their encoded protein is 58 amino *F acids longer. These exons are present in the Celera sequence and generate *F an identical protein. Please amend the CDS feature of AE003418; AAF45553.1. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129060 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG10138 on Tue May 9 03:37:36 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 11:32:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 11:32:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 03:37:36 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10138 on Tue May 9 03:37:36 2000 *F Content-Length: 483 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10138 *F Gene annotation error *F Genes CG10138 and PpD5 should be merged. *F Comments: CG10138 and PpD5 are the same gene: *F Celera sequence AE003456; AAF46787.1 and PpD5 sequence S39961; AAB22464.1 *F from Chen et al, FEBS Lett. 306:54-58(1992) match 100% for amino acids *F 83-106 with respect to the Celera translation. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129061 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG8822 on Tue May 9 05:23:38 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 13:23:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 13:23:58 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 05:23:38 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG8822 on Tue May 9 05:23:38 2000 *F Content-Length: 480 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8822 *F Gene annotation error *F Genes CG8822 and PpD6 should be merged. *F Comments: CG8822 and PpD6 are the same gene: *F Celera sequence AE003581; AAF51146.1 and PpD6 sequence S39995; CAB27426.1 *F from Chen et al, FEBS Lett. 306:54-58(1992) match 100% for amino acids *F 90-113 with respect to the Celera translation. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129062 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG4406 on Tue May 9 05:37:23 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 13:39:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 13:39:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 05:37:23 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4406 on Tue May 9 05:37:23 2000 *F Content-Length: 794 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4406 *F Gene annotation error *F Gene EG:133E12.3 has incorrect exon/intron structure. *F Comments: EG:133E12.3 was isolated by EDGP: *F AL009192; CAA15687.1 *F They define an upstream possible initiating Met so their encoded protein is 5 *F amino acids longer. These codons are present in the Celera sequence and *F generate an identical protein. Please amend the CDS feature of AE003422; *F AAF45703.1. *F FT CDS join(complement(243135..243709), *F FT complement(242939..243056),complement(242776..242886), *F FT complement(242550..242714)) *F amend first exon to read: *F complement(243135..243721) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129063 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG3396 on Tue May 9 06:40:05 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 14:35:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 14:35:15 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 06:40:05 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3396 on Tue May 9 06:40:05 2000 *F Content-Length: 585 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3396 *F Gene annotation error *F Genes CG3396 and CG5138 should be merged. *F Comments: CG5138 *F AE003532; AAF49692.1 *F FT CDS complement(241751..242200) *F CG3396 *F AE003532; AAF49691.1 *F FT CDS join(complement(242771..242863), *F FT complement(242446..242635),complement(241751..242325)) *F CDS features show these two genes are in fact alternatively spliced isoforms *F of the same gene. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129064 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG5277 on Tue May 9 07:14:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 15:11:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 15:11:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 07:14:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5277 on Tue May 9 07:14:41 2000 *F Content-Length: 545 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5277 *F Gene annotation error *F Gene Ip259 has incorrect exon/intron structure. *F Comments: CDS feature for Ip259: *F AE003628; AAF52940.1 *F FT CDS 171868..172629 *F please amend the base span to include the upstream possible initiating *F Met *F FT CDS 171850..172629 *F This longer sequence is confirmed by U56257; AAC32928.1 *F Mottus et al, Gene 198:229-236(1997) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129065 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG5442 on Tue May 9 07:59:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 16:17:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 16:17:16 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 07:59:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5442 on Tue May 9 07:59:27 2000 *F Content-Length: 421 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5442 *F Gene annotation error *F Genes CG5442 and SC35 should be merged. *F Comments: Celera translation of CG5442 AE003636; AAF53192.1 has 100% identity to *F SC35 (AF232775; AAF43415.1, unpublished by Bourbon et al). *F I guess these genes should be merged. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129066 *a Whitfield *b E. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG14795 on Tue May 9 08:46:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 16:42:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 16:42:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 08:46:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG14795 on Tue May 9 08:46:18 2000 *F Content-Length: 880 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG14795 *F Gene annotation error *F Gene EG:196F3.3 has incorrect exon/intron structure. *F Comments: Comparison to EDGP sequence (AL031024; CAA19831.1) reveals the *F Celera translation *F is 12 amino acids too short at the N terminus. I have translated the DNA and *F found *F the missing 2 exons that match exactly to the EDGP sequence. *F Please update the CDS feature of AE003421; AAF45641.1. *F FT CDS join(complement(51985..52090),complement(51737..51919), *F FT complement(51620..51681)) *F > *F FT CDS join(complement(52173..52175),complement(51985..52123), *F FT complement(51737..51919),complement(51620..51681)) *F please note mRNA feature will also need to be updated *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129067 *a Crane-Robinson *b C. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report for CG12098 on Tue May 9 11:59:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 19:54:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 19:54:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 11:59:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: colyn.crane-robinson@port.ac.uk *F Subject: FlyBase error report for CG12098 on Tue May 9 11:59:27 2000 *F Content-Length: 302 *F Error report from Colyn Crane-Robinson (colyn.crane-robinson@port.ac.uk) *F Gene or accession: CG12098 *F Missed gene *F Comments: CG12098 is Sox100B as reported in Mechanisms of Development (2000) *F 93, 185-188. *F Browser: Mozilla/4.04 (Macintosh; I; PPC, Nav) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129068 *a Whitfield *b E. *t 2000.5.10 *T personal communication to FlyBase *u FlyBase error report for CG7355 on Wed May 10 01:21:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 10 09:16:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 May 2000 09:16:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 May 2000 01:21:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG7355 on Wed May 10 01:21:17 2000 *F Content-Length: 699 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7355 *F DNA error at position 117962. Upstream nucleotides: aatctgatcttggtatttta *F Deletion of length 1: g. *F Corrected sequence: ggtattttagttgtg. *F Comments: Celera sequence AE003530; AAF49601.1 encodes a protein 95 amino *F acids long. *F Eig71Eb has also been sequenced by Wright et al, J. molec. Biol. 1996 255(3): *F 387--400. They sequenced a cDNA and genomic DNA and both DNA sequences are *F identical and encode a 105 amino acid protein. Celera, by comparison, has a *F deletion of a G base so reads through to an earlier stop codon. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129069 *a Whitfield *b E. *t 2000.5.10 *T personal communication to FlyBase *u FlyBase error report for CG11992 on Wed May 10 02:31:30 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 10 10:26:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 May 2000 10:26:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 May 2000 02:31:34 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11992 on Wed May 10 02:31:30 2000 *F Content-Length: 436 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11992 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene Rel. *F Comments: Could you please add a CDS feature for the known shorter isoform of *F Rel. *F It is published in Mol. Cell 4:827-837(1999) *F sequence is: AF186073; AAF07932.1 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129070 *a Whitfield *b E. *t 2000.5.10 *T personal communication to FlyBase *u FlyBase error report for CG2829 on Wed May 10 04:06:34 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 10 12:01:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 May 2000 12:01:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 May 2000 04:06:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2829 on Wed May 10 04:06:34 2000 *F Content-Length: 690 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2829 *F Gene annotation error *F Gene BcDNA:GH07910 has incorrect exon/intron structure. *F Comments: BcDNA:GH07910 cDNA has been sequenced by Berkeley, Rubin et al, Aug *F 1999 *F sequence AF181637; AAD55423.1. *F This cDNA differs at the N-terminus. *F It seems there may be alternative 5' exon(s). Could you please see if *F the Celera sequence can translate to make the cDNA. *F Also maybe the 3' end of the gene has been sequenced now? Celera CDS *F feature of AE003428; AAF45892.1 and AAF45893.1 is annotated as an N- *F terminal fragment. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129071 *a Whitfield *b E. *t 2000.5.10 *T personal communication to FlyBase *u FlyBase error report for CG18455 on Wed May 10 05:28:34 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 10 13:24:34 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 May 2000 13:24:34 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 May 2000 05:28:34 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18455 on Wed May 10 05:28:34 2000 *F Content-Length: 731 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18455 *F Gene annotation error *F Gene Optix has incorrect exon/intron structure. *F Comments: CDS feature for Optix is annotated as a fragment: *F AE003839; AAF59147.1 *F Comparison to Seo et al, Mech. Dev. 83:127-139(1999), identifies the 3' exon, *F sequence is AF099184; AAD39863.1 *F The Celera sequence does contain a base pair difference to AF099184 such that *F the stop codon in AF099184 translates to an E (Glu) and translation continues *F for a further 33 amino acids, the total aa length becoming 324 aa. *F Please amend the CDS feature to translate this 324 aa protein. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129072 *a Zinn *b K. *t 2000.5.11 *T personal communication to FlyBase *u FlyBase error report for CG9921 on Wed May 10 22:27:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 11 06:23:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 11 May 2000 06:23:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 May 2000 22:27:53 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: zinnk@its.caltech.edu *F Subject: FlyBase error report for CG9921 on Wed May 10 22:27:53 2000 *F Content-Length: 537 *F Error report from Kai Zinn (zinnk@its.caltech.edu) *F Gene or accession: CG9921 *F Gene annotation error *F Gene CG9921 has incorrect exon/intron structure. *F Comments: there may be a problem with CG9921 and CG9919; the entire protein *F sequence of CG9921 appears to be included in the N-terminus of CG9919. when *F you run the DNA sequence of CG9921, however, it only finds one sequence; so *F perhaps CG9921 is really just a part of CG9919. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129073 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG18608 on Mon May 15 03:05:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 11:00:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 11:00:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 03:05:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18608 on Mon May 15 03:05:37 2000 *F Content-Length: 2288 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18608 *F Gene annotation error *F Gene CG18608 has a mistake in the supporting evidence or functional assignment. *F Comments: prod has been sequenced by Celera as is shown to be gene CG15107 *F (AE003797; AAF57615). *F I believe the correct annotation for prod is gene CG18608 (AE003797; *F AAF57616) (the adjacent CDS on the same strand). *F Full length prod has been sequenced by Torok et al, Genetics 135:71-80 *F (1993) (sequence: U83596; AAC32727.1) and Itoh and Yu have sequenced *F a C-terminal fragment (sequences AB018794; BAA76307 and AB022040; *F BAA82621). *F These sequences align 100% to CG18608. *F Please update the annotation to correct the gene symbols *F clustal of CG18608 to U83596; AAC32727.1 *F Q 1 MNRRNKRTSYYQYEVYLDFMEENPPMSANKLSRTQDGKKWKELSDLLNKCSTGPTLSPEE *F MNRRNKRTSYYQYEVYLDFMEENPPMSANKLSRTQDGKKWKELSDLLNKCSTGPTLSPEE *F T 1 MNRRNKRTSYYQYEVYLDFMEENPPMSANKLSRTQDGKKWKELSDLLNKCSTGPTLSPEE *F Q 61 WRKRLNDWKNSTRSKYRRSINSDDKSNAMTPLENRALQIFSLEPNFREGISMRLHELMEE *F WRKRLNDWKNSTRSKYRRSINSDDKSNAMTPLENRALQIFSLEPNFREGISMRLHELMEE *F T 61 WRKRLNDWKNSTRSKYRRSINSDDKSNAMTPLENRALQIFSLEPNFREGISMRLHELMEE *F Q 121 QEELDEEENENLEELVDEEEEEEVQYQQFIAEPHEQANPTIINGHSAAKKLRLDGSSEII *F QEELDEEENENLEELVDEEEEEEVQYQQFIAEPHEQANPTIINGHSAAKKLRLDGSSEII *F T 121 QEELDEEENENLEELVDEEEEEEVQYQQFIAEPHEQANPTIINGHSAAKKLRLDGSSEII *F Q 181 YEVADVTSDQSAAKEPSAFYGEKIQEQLKRISDIHEASLHFKIARFKYNNPGFEYVPEL *F YEVADVTSDQSAAKEPSAFYGEKIQEQLKRISDIHEASLHFKIARFKYNNPGFEYVPEL *F T 181 YEVADVTSDQSAAKEPSAFYGEKIQEQLKRISDIHEASLHFKIARFKYNNPGFEYVPEL *F clustal of CG18606 to CG15107 *F Q 1 MNRRNKRTSYYQYEVYLDFMEENPPMSANKLSRTQD----GKKWKELSDLLNKCSTGPTL *F M+RR KRTS QY++Y+D ME \+P \+ \++ R D KKWKELSD LNKCS+GPTL *F T 5 MDRRKKRTSSEQYQMYIDMMESDPIFATGRVPRDYDLNYLTKKWKELSDRLNKCSSGPTL *F Q 57 SPEEWRKRLNDWKNSTRSKYRRSINSDDKSNAMTPLENRALQIFSLEPNFREGISMRLHE *F \+PEEWRKRLNDWKN+TR KYRRS+ S \+K \+MT \+E RAL \+F P \+ L *F T 65 TPEEWRKRLNDWKNTTRCKYRRSLLSTEKDISMTSVETRALDLFGKVPTTGGETMLNLKS *F Q 117 LMEEQEELDEEENENLEELVDEEEEEEVQ *F \+E \++ EE \+ \+E \+ V+ *F T 125 EKDEHDDEMEELGQRTSVAFQKELQAAVE *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129074 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG12372 on Mon May 15 03:48:49 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 11:44:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 11:44:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 03:48:49 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12372 on Mon May 15 03:48:49 2000 *F Content-Length: 641 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12372 *F Gene annotation error *F Gene spt4 has incorrect exon/intron structure. *F Comments: spt4 has been sequenced by FBrf0111836 == Chiang et al., 1999, *F Genetics 153(3): *F 1313--1316 (sequence, genomic and cDNA, AF109133; AAF14224 and AF108353; *F AAF14223). *F Celera sequence AE003821; AAF58482 begins at position 42 of the above sequence. *F Translation of Celera sequence reveals the upstream 41 codons are present. *F Please amend the CDS feature to encode the longer protein. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129075 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG9456 on Mon May 15 05:01:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 12:56:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 12:56:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 05:01:54 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9456 on Mon May 15 05:01:53 2000 *F Content-Length: 391 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9456 *F Gene annotation error *F Genes CG9456 and sp1 should be merged. *F Comments: Protein alignment of sp1 (AJ251744; CAB63096) and CG9456 (AE003790; *F AAF57408) is 100% identical, so I guess these genes should be merged! *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129076 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG9456 on Mon May 15 05:08:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 13:03:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 13:03:47 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 05:08:40 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9456 on Mon May 15 05:08:39 2000 *F Content-Length: 794 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9456 *F Gene annotation error *F Gene CG9456 has incorrect exon/intron structure. *F Comments: Second update for this gene! *F The published sequence for sp1 is longer than the Celera sequence by N-terminal *F 46 amino acids. These upstream amino acids can be found in the Celera sequence. *F The mRNA exon-intron structure is wrong so cannot be used to find the upstream *F sequence. Translation of upstream amino acids 6-46 are found in frame with *F Celera initiating Met and amino acids 1-5 are on an further upstream exon. *F I have not worked out coordinates (sorry). *F Sequences are: *F sp1: AJ251744; CAB63096 *F CG9456: AE003790; AAF57408 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129077 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG3939 on Mon May 15 05:46:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 13:41:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 13:41:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 05:46:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3939 on Mon May 15 05:46:18 2000 *F Content-Length: 384 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3939 *F Gene annotation error *F Genes CG3939 and EG:140G11.5 should be merged. *F Comments: CG3939 sequence (AE003426; AAF45851) and EG:140G11.5 sequence *F (AL035395; CAA23057) align 100% so I guess should be merged! *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129078 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG3939 on Mon May 15 05:51:48 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 13:47:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 13:47:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 05:51:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3939 on Mon May 15 05:51:48 2000 *F Content-Length: 555 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3939 *F Gene annotation error *F Gene CG3939 has incorrect exon/intron structure. *F Comments: CG3939 sequence (AE003426; AAF45851) is 12 amino acids shorter at the *F N-terminus than EG:140G11.5 sequence (AL035395; CAA23057). These amino *F acids are present in frame upstream of the Met defined by the CDS *F feature. The coordinates of the longer CDS are those the mRNA feature *F translates. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129079 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG3708 on Mon May 15 06:13:49 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 14:09:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 14:09:05 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 06:13:49 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3708 on Mon May 15 06:13:49 2000 *F Content-Length: 624 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3708 *F Gene annotation error *F Gene EG:BACR7A4.18 has incorrect exon/intron structure. *F Comments: EG:BACR7A4.18 corresponds to CG3708 (known in FlyBase genes file, but *F not in GadFly). *F EG:BACR7A4.18 sequence (AL109630; CAB65874) is 13 amino acids longer *F than CG3708 sequence (AE003419; AAF45565). The upstream aa are present *F so please amend the CDS feature: *F FT CDS complement(83014..84102) *F > *F FT CDS complement(83014..84141) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129080 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG14625 on Mon May 15 06:39:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 14:34:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 14:34:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 06:39:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG14625 on Mon May 15 06:39:26 2000 *F Content-Length: 582 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG14625 *F Gene annotation error *F Gene EG:BACR42I17.8 has incorrect exon/intron structure. *F Comments: EG:BACR42I17.8 corresponds to CG14625 (known in FlyBase genes file, *F but *F not in GadFly). *F EG:BACR42I17.8 sequence (AL121806; CAB65885) is 18 amino acids longer *F than CG14625 sequence (AE003420; AAF45592). The upstream aa are *F present in an upstream exon so please amend the CDS feature (no *F coordinates, sorry!). *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129081 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG4006 on Mon May 15 07:14:40 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 15:10:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 15:10:09 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 07:14:40 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4006 on Mon May 15 07:14:40 2000 *F Content-Length: 976 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4006 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene Akt1. *F Comments: Celera is missing the annotation for the longer isoform of Akt1. *F Andjelkovic et al., 1995 *F Developmental regulation of expression and activity of multiple forms of the *F Drosophila RAC protein *F kinase. J. Biol. Chem. 270(8): 4066--4075 FBrf0079853 *F submitted two sequences, one for each isoform. *F The shorter isoform, X83510; CAA58500, corresponds to CG4006, AE003711; *F AAF55275. The longer isoform is not annotated, and the sequence is *F present for correct translation (no coordinates, sorry). *F Please note that the N-terminal codon of the longer isoform, X83510; *F CAA58499 is annotated as a translation exception in the EMBL nucleotide *F database, and therefore the ACG enocdes a Met, not a Thr. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129082 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG10244 on Mon May 15 08:08:49 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 16:04:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 16:04:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 08:08:49 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10244 on Mon May 15 08:08:49 2000 *F Content-Length: 457 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10244 *F Gene annotation error *F Gene CG10244 has incorrect exon/intron structure. *F Comments: CG10244 has a cadherin domain at the N terminus ans a protein kinase *F domain at the C terminus. No known cadherin also carries a kinase *F domain so could you please re-evaluate the structure. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129083 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG15511 on Mon May 15 08:16:47 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 16:11:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 16:11:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 08:16:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG15511 on Mon May 15 08:16:47 2000 *F Content-Length: 617 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG15511 *F Gene annotation error *F Gene CG15511 has incorrect exon/intron structure. *F Comments: CG15511 has one cadherin repeat and is very short (258 aa). Most of *F the *F mammalian cadherins in Swiss-Prot are ~1000 aa and all of the other *F known Drosophila entries are anything from 1000-5000 aa. The adjacent *F gene, CG7805, has a number of cadherin repeats along its entire length *F so it seems likely that CG15511 and CG7805 could be a single gene. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129084 *a Jin *b Z. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG10569 on Mon May 15 17:29:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 01:24:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 01:24:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 17:29:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: zjin@ualberta.ca *F Subject: FlyBase error report for CG10569 on Mon May 15 17:29:46 2000 *F Content-Length: 827 *F Error report from Zhigang Jin (zjin@ualberta.ca) *F Gene or accession: CG10569 *F cDNA or EST error *F Comments: The size of CG10569 transcript is 2311. However, using the cDNA *F sequence based on our prediction as a probe, we only detected one band of *F approximately *F (smaller than) 2Kb from samples on different developmental stages. *F Our prediction has four exons instead of 7. The first three exons are exactly *F the same. The fourth of ours is from 36046 to 36472 (the numbers refer to that *F of AE003565 genomic DNA sequence), whereas the BDGP annotation predicts that *F it's from 36046 to 36177. The difference of this exon in turn determines the *F difference of the size of the transcript, because our prediction has a stop *F codon. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129085 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG12630 on Tue May 16 06:54:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 14:50:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 14:50:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 06:54:43 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12630 on Tue May 16 06:54:43 2000 *F Content-Length: 377 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12630 *F Gene annotation error *F Genes CG12630 and tiptop should be merged. *F Comments: Sequence is identical for tiptop (AF219383; AAF23183) and Celera *F CG12630 (AE003782; AAF57242), please merge the genes. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129086 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG12630 on Tue May 16 06:57:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 14:52:48 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 14:52:48 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 06:57:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12630 on Tue May 16 06:57:32 2000 *F Content-Length: 708 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12630 *F Gene annotation error *F Gene CG12630 has incorrect exon/intron structure. *F Comments: The submitted sequence for tiptop (AF219383; AAF23183) is longer *F than the *F Celera sequence for CG12630 (AE003782; AAF57242) by N-terminal 39 amino acids. *F These upstream amino acids can be found in the Celera sequence. Translation of *F upstream amino acids 18-39 are found in frame with Celera initiating Met and *F amino acids 1-17 are on an further upstream exon. *F I have not worked out coordinates (sorry). *F Please amend the CDS and mRNA feature. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129087 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG9943 on Tue May 16 07:29:35 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 15:24:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 15:24:57 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 07:29:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9943 on Tue May 16 07:29:35 2000 *F Content-Length: 373 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9943 *F Gene annotation error *F Genes Surf1 and CG9943 should be merged. *F Comments: Sequence is identical for Surf1 (AF182954; AAF19610) and Celera *F CG9943 (AE003560; AAF50632), please merge the genes. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129088 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG9943 on Tue May 16 07:35:03 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 15:30:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 15:30:09 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 07:35:03 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9943 on Tue May 16 07:35:03 2000 *F Content-Length: 923 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9943 *F Gene annotation error *F Gene Surf1 has incorrect exon/intron structure. *F Comments: The submitted sequence for Surf1 cDNA (AF182954; AAF19610) is longer *F than the *F Celera gene CG9943 (AE003560; AAF50632) by N-terminal 7 amino acids. *F These upstream amino acids can be found in the Celera sequence in frame with *F Celera initiating Met. *F Please amend the CDS and corresponding mRNA feature. *F FT CDS join(complement(250739..250921), *F FT complement(250549..250670),complement(250292..250486), *F FT complement(249854..250235)) *F > *F FT CDS join(complement(250739..250942), *F FT complement(250549..250670),complement(250292..250486), *F FT complement(249854..250235)) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129089 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG16857 on Tue May 16 07:46:36 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 15:41:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 15:41:58 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 07:46:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG16857 on Tue May 16 07:46:36 2000 *F Content-Length: 815 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG16857 *F Gene annotation error *F Gene BcDNA:GH11322 has incorrect exon/intron structure. *F Comments: Alignment of Berkeley cDNA BcDNA:GH11322 (AF181644; AAD55430) to *F Celera CG16857 *F (AE003576; AAF51028) identifies a possible upstream initiating Met 25 amino *F acids upstream and in frame of the Celera init Met. *F please amend the CDS feature: *F FT CDS join(complement(291117..291263), *F FT complement(286801..287308),complement(286326..286735), *F FT complement(285705..285933),complement(285284..285490), *F FT complement(284632..285215)) *F first exon should read: *F complement(291117..291338) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129090 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG4272 on Tue May 16 07:58:34 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 15:54:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 15:54:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 07:58:34 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4272 on Tue May 16 07:58:34 2000 *F Content-Length: 660 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4272 *F Gene annotation error *F Gene BcDNA:GH09817 has incorrect exon/intron structure. *F Comments: Alignment of Berkeley cDNA BcDNA:GH09817 (AF181640; AAD55426) to *F Celera CG4272 *F (AE003583; AAF51264) identifies a possible upstream initiating Met 20 amino *F acids upstream and in frame of the Celera init Met. *F please amend the CDS feature: *F FT CDS join(complement(166532..168364),complement(165764..166429) *F FT ) *F first exon should read: *F complement(166532..168424) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129091 *a Ashburner *b M. *c T. *d Benos *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG7981 on Tue May 16 11:03:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 18:58:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 18:58:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 11:03:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG7981 on Tue May 16 11:03:14 2000 *F Content-Length: 988 *F Error report from m. ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG7981 *F Missed gene *F Comments: This may be three genes, corresponding to EG:BACR25B3.11 (= pcan), *F EG:BACR25B3.10 and EG:BACR25B3.1. Itneeds to be sorted out. This *F from Takis Benos: *F >From benos@ebi.ac.uk Sat May 06 19:47:13 2000 *F That was the difficult gene i annotated in February; do you rember? My *F notes say that i'm not sure whether this is the correct structure of the *F locus. However, it is definetely NOT one gene. I cannot see the actual *F alignments with the protein hits right now (due to technicalities of EBI), *F but i remember that the same protein regions hit in more than one place the *F Drosophila sequence. *F Now, recalling how the CloneView connects all together, and the fact that *F is difficult to find out the correct regions through it, i am not surprised *F that Celera put all together in one gene. *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129092 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG18466 on Wed May 17 01:41:24 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 09:36:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 09:36:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 01:41:24 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18466 on Wed May 17 01:41:24 2000 *F Content-Length: 803 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18466 *F Gene annotation error *F Gene Nmdmc has incorrect exon/intron structure. *F Comments: Hedengren et al, Mol. Cell 4:827-837(1999) sequence AF186073; AAF07929 *F identify a longer isoform that translates a further 6 amino acids upstream. *F Celera sequence AE003681; AAF54332 translates the shorter isoform (isoform B). *F To translate the longer isoform (isoform A) a CDS feature should be defined *F which will show the mRNA feature should be amended as it does not include the *F upstream exon: *F longer isoform: *F FT CDS join(complement(141831..141844),complement(134319..135102), *F complement(134124..134251) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129093 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG2668 on Wed May 17 02:04:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 09:59:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 09:59:46 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 02:04:46 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2668 on Wed May 17 02:04:45 2000 *F Content-Length: 670 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2668 *F Gene annotation error *F Gene BcDNA:GH06048 has incorrect exon/intron structure. *F Comments: The mRNA feature of BcDNA:GH06048 (AE003466; AAF47319) defines a *F longer protein *F than that translated by the CDS feature. This longer translation is also *F provided by the Berkeley cDNA translation AF184225; AAD55736. *F please amend the CDS feature: *F FT CDS complement(200107..201120) *F > *F FT CDS join(complement(201287..201301),complement(200107..201225) *F FT ) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129094 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG8114 on Wed May 17 02:32:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 10:27:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 10:27:01 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 02:32:03 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG8114 on Wed May 17 02:32:02 2000 *F Content-Length: 832 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8114 *F Gene annotation error *F Gene pbl has incorrect exon/intron structure. *F Comments: Prokopenko et al, Genes Dev. 13:2301-2314(1999) haveisolated a cDNA *F that encodes *F a protein (AF136492; AAD52845) just 2 amino acids longer than that defined by *F Celera (AE003557; AAF50508). Please amend the CDS feature to extend to the *F more upstream possible initiating Met. *F FT CDS join(139835..139865,143949..144165,149012..149147, *F FT 149222..149328,149423..149672,152433..152564, *F FT 152634..152984,153048..153989,154055..154165, *F FT 154226..154375,154435..154563) *F replace first exon with: *F 139829..139865 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129095 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG5133 on Wed May 17 02:47:25 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 11:28:44 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 11:28:44 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 02:47:25 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5133 on Wed May 17 02:47:25 2000 *F Content-Length: 636 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5133 *F Gene annotation error *F Gene Dorsocross has incorrect exon/intron structure. *F Comments: FBrf0118195 == Murakami, 1999.12.1, GenBank/EMBL/DDBJ: AB035412 *F submitted a cDNA of dorsocross. *F Using this cDNA the Celera sequence of Dorsocross (AE003553; AAF50328) can be *F updated to translate the N terminus of the protein (it is currently annotated *F as a C terminal fragment). *F The sequence is there but I did not work out the coordinates for the upstream *F exon, sorry. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129096 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG6258 on Wed May 17 03:37:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 11:32:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 11:32:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 03:37:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6258 on Wed May 17 03:37:17 2000 *F Content-Length: 719 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6258 *F Gene annotation error *F Gene BcDNA:LD06837 has incorrect exon/intron structure. *F Comments: BcDNA:LD06837 cDNA sequence from Berkeley (AF160912; AAD46852) *F demonstrates the *F Celera translation (AE003631; AAF53076) is missing the N terminal 38 amino *F acids. *F Unfortunately the mRNA feature also has errors so cannot be used to make the *F correct translation. The DNA is there to translate the upstream amino acids, *F . *F . *F Please correct both the CDS and mRNA features *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129097 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG8614 on Wed May 17 03:50:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 11:45:17 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 11:45:17 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 03:50:08 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG8614 on Wed May 17 03:50:08 2000 *F Content-Length: 848 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8614 *F Gene annotation error *F Gene Neos has incorrect exon/intron structure. *F Comments: BcDNA:HL05936 (Neos) cDNA sequence from Berkeley (AF160904; AAD46844) *F demonstrates the Celera translation (AE003559; AAF50595) is missing the *F N terminal 80 amino acids. The amino acids are upstream in frame of *F the defined initiating Met, so the translationshould be extended to *F start from the first available Met to generate the longest protein. *F Please amend the CDS feature: *F FT CDS join(complement(236393..236574),complement(235636..236329) *F FT ) *F > *F FT CDS join(complement(236393..236814),complement(235636..236329) *F FT ) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129098 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG9124 on Wed May 17 03:56:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 11:52:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 11:52:05 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 03:56:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9124 on Wed May 17 03:56:58 2000 *F Content-Length: 850 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9124 *F Gene annotation error *F Gene BcDNA:GM14618 has incorrect exon/intron structure. *F Comments: BcDNA:GM14618 cDNA sequence from Berkeley (AF160896; AAD46836) *F demonstrates the *F Celera translation (AE003608; AAF52210) is missing the N terminal 2 amino *F acids. The amino acids are upstream in frame of the defined initiating Met, so *F the translation should be extended to start from the first available Met to *F generate the longest protein. *F Please amend the CDS feature: *F FT CDS join(complement(111166..111831),complement(110842..111099) *F FT ) *F > *F FT CDS join(complement(111166..111837),complement(110842..111099) *F FT ) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129099 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG11194 on Wed May 17 04:11:04 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 12:06:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 12:06:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 04:11:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11194 on Wed May 17 04:11:04 2000 *F Content-Length: 808 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11194 *F Gene annotation error *F Gene Hey has incorrect exon/intron structure. *F Comments: Hey cDNA sequence (AF151523; AAD46771, Kokubo et al, Biochem. Biophys. *F Res. Commun. 260:459-465(1999)) demonstrates the Celera translation *F (AE003839; AAF59152) is missing the N terminal 4 amino acids. The amino *F acids are upstream in frame of the defined initiating Met, so the *F translation should be extended to start from the first available Met to *F generate the longest protein. *F Please amend the CDS feature: *F FT CDS join(46250..46497,46911..47125,47867..48669) *F > *F FT CDS join(46258..46497,46911..47125,47867..48669) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129100 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG12846 on Tue May 16 05:15:13 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 13:10:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 13:10:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 05:15:13 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12846 on Tue May 16 05:15:13 2000 *F Content-Length: 404 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12846 *F Gene annotation error *F Genes CG12846 and BcDNA:GH07074 should be merged. *F Comments: Sequence is identical for Berkeley cDNA BcDNA:GH07074 (AF220042; *F AAF23826) and Celera *F CG12846 (AE003842; AAF59312), please merge the genes. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129101 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG12846 on Tue May 16 05:15:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 13:11:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 13:11:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 05:15:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12846 on Tue May 16 05:15:57 2000 *F Content-Length: 662 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12846 *F Gene annotation error *F Gene CG12846 has incorrect exon/intron structure. *F Comments: The submitted sequence for BcDNA:GH07074 is longer than the Celera *F sequence by *F N-terminal 32 amino acids. These upstream amino acids can be found in the *F Celera *F sequence. Translation of upstream amino acids 21-32 are found in frame with *F Celera initiating Met and amino acids 1-20 are on an further upstream exon. *F I have not worked out coordinates (sorry). *F Please amend the CDS and mRNA feature. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129102 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG9075 on Wed May 17 05:34:52 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 13:30:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 13:30:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 05:34:52 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9075 on Wed May 17 05:34:52 2000 *F Content-Length: 681 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9075 *F Gene annotation error *F Gene eIF-4a has incorrect exon/intron structure. *F Comments: Berkeley cDNA of eIF-4a (AF145621; AAD38596) encodes a longer *F protein than that *F translated by the Celera sequence (AE003612; AAF52317), defining 14 upstream *F amino acids. Translation of the N terminal exon defined by the mRNA feature *F translates 13 of the 14 amino acids and 1 base. The remaining 2 bases of the *F initiating Met are on an upstream exon, I do not have coordinates, sorry. *F Please update the CDS feature *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129103 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG5371 on Wed May 17 06:36:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 14:31:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 14:31:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 06:36:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5371 on Wed May 17 06:36:20 2000 *F Content-Length: 775 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5371 *F Gene annotation error *F Gene RnrL has incorrect exon/intron structure. *F Comments: The mRNA feature of RnrL (AE003628; AAF52913) defines a longer protein *F than that translated by the CDS feature. This longer translation is also *F provided by the Berkeley cDNA translation AF132143; AAD33590. *F please amend the CDS feature: *F FT CDS join(complement(90771..90788),complement(90361..90538), *F FT complement(88183..90302)) *F > *F FT CDS join(complement(90911..90962),complement(90771..90859), *F FT complement(90361..90538),complement(88183..90302)) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129104 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG5371 on Wed May 17 06:48:06 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 14:43:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 14:43:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 06:48:06 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5371 on Wed May 17 06:48:06 2000 *F Content-Length: 764 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5371 *F DNA error at position 90851. Upstream nucleotides: atcgaagtgcacctcctcct *F Substitution: base g should be c. *F Comments: There is a conflict between Berkeley cDNA translation of RnrL *F (AF132143; *F AAD33590) and Celera translation (AE003628; AAF52913). *F Conflict exists at amino acid 21 with respect to the cDNA sequence (this amino *F acid resides in the currently untranslated Celera sequence as the CDS feature *F needs to be updated, update already sent). *F Please check the nucleotide sequence at this position as the strain used for *F both sequences is the same \- one of you has got it wrong! *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129105 *a Whitfield *b E. *t 2000.5.19 *T personal communication to FlyBase *u FlyBase error report for CG3479 on Fri May 19 02:32:49 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri May 19 10:27:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 19 May 2000 10:27:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 19 May 2000 02:32:49 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3479 on Fri May 19 02:32:49 2000 *F Content-Length: 759 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3479 *F Gene annotation error *F Gene CG3479 has incorrect exon/intron structure. *F Comments: osp gene has been examined by Ashburner et al, Genetics *F 153:179-219(1999) and *F using genomic and cDNA data they have identified two alternatively spliced *F isoforms of osp: *F AE003410; AAF44880 (long) *F AE003410; AAF44881 (short). *F The sequence from the Celera entry AE003644; AAF53402 appears to be a *F combination of exons from both isoforms. As the same strain has been used *F for both sequences could you please update the CDS feature to represent *F the long isoform and add a CDS feature for the short. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129106 *a Millburn *b G. *t 2000.5.23 *T personal communication to FlyBase *u FlyBase error report for CG16917 on Tue May 23 09:06:38 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 23 17:02:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 23 May 2000 17:02:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 23 May 2000 09:06:38 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG16917 on Tue May 23 09:06:38 2000 *F Content-Length: 1330 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG16917 *F Gene annotation error *F Genes CG16917 and cana (CG4831) (maybe) should be merged. *F Comments: Hi, *F in the course of curating a FlyBase error report *F (E.A. Greene, 2000.4.11, personal communication to FlyBase \- FlyBase error *F report for CG4831 on Tue Apr 11 15:31:18 2000.) *F that merges cana (FBgn0040233) with CG4831 (FBgn0032356), I have noticed that *F it is possible that CG16917 should maybe also be merged with cana. *F The mRNA sequence for cana (AF220354) overlaps with the genomic scaffold *F sequence AE003631. *F The region of overlap corresponds to CG4831, and also (towards the 3' end of *F the cana *F mRNA sequence) to CG16917, so it is possible that CG16917 is in fact a 3' part *F of cana. *F However, the CG16917 transcripts overlap cana (AF220354) from approx bp 6620 *F of the cana *F sequence onwards. This is after the CDS of cana as annotated in AF220354 (CDS *F goes from *F 178..5973) and yet one of the transcripts of CG16917 does have a translation. *F I therefore wasn't sure whether cana and CG16917 should be merged or not. *F I will send to flybase-updates the ClustalW alignment of the 2 CG16917 *F transcripts *F and the cana mRNA sequence (AF220354). *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Tue May 23 17:04:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 23 May 2000 17:04:35 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: alignment for CG16917 FB-error report *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 23 May 2000 17:07:55 \+0100 *F Content-Length: 58037 *F Here is the alignment of cana (AF220354) and the CG16917 transcripts: *F Pairwise Scores: *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: AF220354 8006 bp *F Sequence 2: CG16917|FBgn0032357|CT37528|FB 973 bp *F Sequence 3: CG16917|FBgn0032357|CT37530|FB 898 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (2:3) Aligned. Score: 97 *F Sequences (1:2) Aligned. Score: 100 *F Sequences (1:3) Aligned. Score: 100 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/19798959097261.dnd *F Start of Multiple Alignment *F There are 2 groups *F Aligning... *F Group 1: Sequences: 2 Score:16581 *F Group 2: Sequences: 3 Score:17284 *F Alignment Score 21049 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/19798959097261.aln *F Your guide tree: *F 19798959097261.dnd *F ( *F AF220354:-0.01336, *F CG16917|FBgn0032357|CT37528|FB:0.01336, *F CG16917|FBgn0032357|CT37530|FB:0.01336); *F Your Multiple Sequence Alignment: *F 19798959097261.aln *F CLUSTAL W (1.8) multiple sequence alignment *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTGGCTCGCGCCTATTTACTGGTTCCGTATTTCGATCTGGTTCGCTTCCG 50 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGCGGTATTTGTTCGAATTTACAAAGAGAAACTAACATGTCGATCAAAC 100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TAGCTTTTATAAAACGTTAAAATCTGTTTGTGTCTGTTCATTGTGCATGT 150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CAAACACGTTTCCCACAAGTCGCAAAGATGTCTACGAAGAACGCCAGCTC 200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CATCCAGGTCTGCATCAAGGTGCGTCCCTGCGAGCCCGGGCTCACTTCAC 250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCTGGCAGGTGAAGGAGGGCCGCTCCATCCAGTTGGCGGACAGCCATGCG 300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAGCCCTATGTCTTCGACTATGTGTTCGACGAGGGCGCCAGCAACCAGGA 350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGTGTTCGACCGGATGGCCAAGCACATAGTGCACGCCTGCATGCAGGGCT 400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCAACGGAACTATTTTTGCCTACGGCCAGACGTCGTCGGGCAAGACGTAC 450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACCATGATGGGCGACGAACAGAATCCGGGCGTCATGGTGCTAGCCGCCAA 500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGAGATCTTTCAACAGATCTCCAGTGAGACGGAGCGGGACTTTCTGCTGC 550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GCGTGGGCTACATCGAGATCTACAACGAGAAGATCTACGATCTGCTGAAC 600 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGAAGAATCAGGACCTCAAGATCCATGAGTCCGGCAATGGGATTGTGAA 650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGTGAACTGCAAGGAGTCCATCGTAACCAGCGAGGACGACCTCCTGCGCC 700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGCTCTACATGGGCAACAAGGAGCGCGTCGTGGGCGAGACCAACATGAAC 750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAGCGGTCCAGTCGTTCGCACGCCATCTTCAGGATAATCATTGAGTCCCG 800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAAGTCGGACCACAGTGATAACGACACCGTAAAGCAGAGCGTACTGAGCC 850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGGTGGATCTGGCTGGATCCGAACAGGTGGACCCCGCGGACCACGCATCC 900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGCCTTATGATTTTTCGCAACTTGGTTAAGAGCCTCTCCGAGAGTGTAGA 950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TAGCAAGCCGAATAGCTTTCGCGATTCGAAACTTCCGCGCATCATGCTGC 1000 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTTCGCTGGGTGGAAATGTTTTGACCTCTATCATCTGTACAATTACGCCA 1050 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCGTTCGTGGAGGAGTCGTCATCCACTATAAGCTTCGGCACGTGTGCCAA 1100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAAAATTCGTTGCAAGCCGCAGGTCTGCAAGATAGACTCTGAAACCACGA 1150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGATGAGGCAACTTGATCGCGGGATCAGTATGTTGAAGGATAAGCTTGCT 1200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGAAGAAGATCAAGAATGAGAGCCAGCTGGTATTGCAGGAATTGGAAGG 1250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCGCATCAAGCGCGACATGTTGAAGATCGTTTCCAGCGCCTCGCTTGACG 1300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACTATCGCCTCCAGAAGCGTCGTCGTACTTGGACTTTAACCGCCTCAGGC 1350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCAGAAGGCGACGCTCCTGTTCTCGCATTGCCTGAGCCAGAGGAGTCCCG 1400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCTACCGCGTCCTTCGAAGCTGACTAACCTGCCGAAACCCTTGTTTCAGC 1450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGCGGGGGATTGCTCCCAAAGCCAAAGGCATTTGTAAAACCCTAAAGGAG 1500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAAAGGTTACAAACGGATAATATGGACACTATGCCTGGCCGGGCGAAACA 1550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GTTGGGAAGAGAGACAGCTAGCAGAATTCCTTCAGTAATGATGTCAAAAA 1600 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGTATCAGGAGTCGGTGCCGAACTGCGATGCCCCGCAGACCGAGATCTCT 1650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GCGCTTACTGCATCCAACCAGGTAGCAAAGGAGACGATCGAGAAGTACGA 1700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGAGCAGGTGAAGAGGCTAAAGGAGACAATTGAGCGGCTGGAGATGGAGA 1750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACGGCAAGGCAGTCAATCTAGGGGAGCAATTCGAAACACACAAGGCCAAG 1800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCGAAGCAAATGGAGGAAGAACTTCTCTCCTCCATCTCCGAAAAAGATTC 1850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GACCATAGTAAGTCTGCAACAGTCCCTTGAGGAGCTATCTCGGGATGTGT 1900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGCGAAACAGCAAGGAGGATCAAATGCGATCCATGTGCCCCGAGCTTGAG 1950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCCAGCTGCGAGCGGATCTGCAACAAGTGCCTGGAGTTGGAACGGCTACT 2000 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GCCCCTAGCGAGTGCCTCAGGACTGGACTCAGTCGCCTGCCAGTTTGATC 2050 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGTTGCGATCGGAGATTGCTGCCACCCGTATGAAGTTGGAAAGTATGCTG 2100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCTACTTTCAGCCATGCAAGCTGTGAGGTATCACAAAAGACCACTGATTG 2150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CAAACGGCTCTCCGAACAGATATCCACAGCGCATGATGACTTTGGACAAT 2200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGCAGGAAAAGTACAACAACCTGAAGCATAAGTGGTCTAGTCAAAAGCTG 2250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GCCATTGATACCATGCAGGTTGACTACAATACTATACAGCAAAAGTACCT 2300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACAGCTGCAGGACGAGTATAGGCATCTGGAACTCAGATCGGATGAACAGT 2350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GTCAGCAGCTGCAGGATGAAAATTCTAAACTGCAAGCCGAGATTGGAACT 2400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTCAAGGAGCGAGTGGAGGAGATCCATAGTGAGCTATTAGAAGTTCCCAA 2450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCCGGATACTCATCCAGAAGATATGGAGCTACAAAATCAAGAGCTGAAGA 2500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGCGCCTCTCCAAGTTGCAGTGGGAGTTTGACGAGATCCAACTGAATTAC 2550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAATGTCTATCCAACGAATTGATGAGTACTATTCAAGAGTGCGATGCACT 2600 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCGAGAGGAGCACAAGCAACGAACAACGAACTCCGATTTGGAATCCATGA 2650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGAGTTCGGGTGTAGGCACTGAGTGCAGTGATCCGGAAAACGAATTGGAC 2700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACTGATCTGCTGCAACAGTTCACCAAGCTGTCGAAGTCCATCCAACAGAT 2750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGAGCTCACCGACTACTCCGGTGGCAGGCGCCTCTTTATATATAACCATG 2800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCGAACAGGATCAAAGCGTGCCAAGCCTAAAACTTTGCCTTGAGCCTGCT 2850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGTATTTAGAGGGTGATGGTAAACAACATGATGCATCCGACTCCGTATT 2900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCTTAAAGGCTTTCTGAAATGCCAAAGATTTCAGATTGTCAAAATAAATC 2950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGGAACAGAATTTGGTGAAAGAGGAAGACCGAATGCGTGATATCATTTTC 3000 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CAACTGAAACAAGAAGTTGACGGGAAAAAGAATCTAATTGAGGAAGAGAA 3050 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGAAGTTATTAACAATTTGCGTGCACAAATTACCAGCCTGAATCAAATCG 3100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAACTATAAAAAATCAAAATGCCAAGACAAAAATATTGTGCGAGGAGTTG 3150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CAGACTAAAGATACCGTACAGACCGCTAACAAGCAGGAATCTCAGGAAGT 3200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCTTACTCTCAAGACATCCCTTGCACATCTGAAGTCCAAGGTGTGTGAAC 3250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGCAGAAAAAACTTGAAAAGCAGTCAGAGGACGAAAAAATATCCGAACTG 3300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CAGTCGGATATTGGAGAAATAAGCGAGTGTTGCTTATCCATGGAACTGAA 3350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GCTAGCAGACATTGTAAATTGGCAGGCGGAGGAGTTGCGCCCTCTAGATC 3400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGCTTCAAGAAAGCGGTGTCGAACTGCAGCACCATAGTACCACAGCTGAA 3450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAATCTCTAAATGTTGAGAAGCCTATCCAGGAACAAACTGAACGAACTCT 3500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TACCACCGAATATGAACGCCGAATCGAGCAACTTGAAGAATCATTACAAA 3550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGGCACAAGAGGAATTAAGTATTCTGGAGAAGCGAAAGACAGATGAGAAC 3600 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAAAGCCTTCAGCTTGAGTACATGGCGAAAATTGAGACGAGCGAGAATGA 3650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAACCGCTCCAAATTCCGTGCATACTGCCTGGATTTAAAGGAAACCCAGA 3700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AACGCTACGAAGAGCAGCTACAGCAGACCAATGAAAAGCTCGCCAGTGTT 3750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACGACTCAGTGTCAGGTGCATTTGGATGTCATTAAAAGATCTCTTCAAGA 3800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAAGATTACTCAGGCCGAGAAGGAAAGAAACGAATTGGCTGTCCGACACA 3850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGGCAGAGCTTGAAAAAATCAGGGAAACGCTGAAGGAGAAGGAATCTTCT 3900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TACAAGGAGAAACTAAGGCAAGCGGAGGAGGAGCGCGATAAGGAAATTTC 3950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TAGGCTTGAGGTGATGCGAAACACAATAGCAGAGCTGCATAAAACGAATT 4000 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTGATCGGGAAGTGGAACTCGAGGGAGTGAAAATGGAAAAATGCCAACTT 4050 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGAAGCTGTACGACAAAAGCATGTTGGAGCTAGAACAACTGCAATGCAC 4100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGCCGACCAGAAAAGTTCGGATTTGCTGCCAGGCTCGTCGAACGAGAACA 4150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCGATGACCTGCAAAAGAAGTGCGATCAATATGTTCAGGATTTGGAGCTG 4200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TTGAGGGGCGAGAAAGCAGAACTGCTATCGGAGATCCAGAAAATCAACGG 4250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACAGCACTCGAATACCATAAAAAAACTTGAGGAAATCGAAGCGGAAATGA 4300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TTACACTAACCACTCAAAAGGAGTTGGAGAGGTGTGAAATTGCAGAAAAG 4350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTGGAGACTTTCAAGTCCAAGGAGGCAGATATCAAAGAAGCTCTGCACTG 4400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGCCCAGCTCAGGCTTCATGCCTACGATAAGCTGGTGTGCGAGTACGAGC 4450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGCTAAAAGGCTGTCTTTCGGATTCAAATAAGTTGTCGGAAAATCTGCAG 4500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGAAAGTGGAGCGTTTGCATGCTGAACAGCTTGCTTTACAAGAAGGAAT 4550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TTCCGGCCGAGATTCAGAGATTAAACAGCTTCGCTCTGAGCTCAAGGACG 4600 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCATTGATGAAAATACAACAGTGAGAGAAGCAAAAGTAGGCCTGGAAAAT 4650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGTCTTAAAGCAGTACAGGAAAATATGTCTGCCCAGGAAAGTCAGTTTAA 4700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACAAAAGATTGCTGATATTAAGGGTTCAGTGGACGAGCTTCAGATTAAAC 4750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TAAAATCTCTGCAAGAAGTAAGAGACCATCTAGAGTCTAGAAATGAAGAG 4800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTTAAAAGAAAGCTAAAAGATGCTCAGGAACTACAGAACATGGTGGATAA 4850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGAGCGAAAGCTCAATTCCTCTCTACGAGAAGATTTTGACAAACTGGAAC 4900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAACCAAACTCGATCTCGAAGAGCAGCTTCGAGCCAAGAAAGTAGAAATC 4950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GATCGCCGATCGAAGGAGCTTGGCGAAGTGACCAAGGATTGCGAAAATAT 5000 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACGGTCCGATCTGGAAGCTCAAACTAATGATTTCCTGAAAGAAAGGGAAA 5050 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTTTGAACTTGACTATATCCGATCTTCGGTTGCATAATGAGCAATTGCTA 5100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAAACCTCGAAAAATTATCTATCAGACATAACAGCAGCAAATAATTTAAA 5150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCTCGAAATGAAGAAGAATCTTCACGATCTCACAAAAGAGTGCAAGAGCT 5200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TACGTTCCGATCGGCAATCCAAAGAAGAATATTTCCAAACGCAAAAGCAA 5250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTGTTGGATGAAACAATTTCTAATCTAAAGGAGGAAAACCGCAAGATGGA 5300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GGAAAAGTTGAGTTCGGGTAACAAGGCGCTCAACGAGGATTGCGAAAAGC 5350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCCGTTCAACATTGGAATCAAAGGAACTTATACTGCAACAGAATAAACAA 5400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAGTTAGAGGAAAGGCTGACTGTTATTAATGAGAAGAATGGGAAAAACGC 5450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACTACTTGACGCCCAGTTGAAATCCAATGAAACCGCCTTTAAATCACTGC 5500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAAAGGCATGGATTAAGCAAAGTTTAGCCATAGAAGCGGCCAATAAAAGA 5550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGCCTCGAGATGGAACAGATGGTTGATAAACGTACGAGGGAGTACGAAGA 5600 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACTACGATCAACTCTGAAAACCAGGGAAATCAACTTTCGATCGGAGAAGG 5650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AACGCATGGACGGCACCATCTCTAGCCTGTTAGAAGACAAGCGTAATCTT 5700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GAGGAGAAGTTGTGCACAGTCACCGAGCTCCTGGCTAAGCTAAAGCGCGA 5750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACTGCCGGCTTTACACACGCAAAAAGTGAACGGAGGCGATGTGTCCATTG 5800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGTTGAATTCCTCGAATGGATCGCCGACTCCTGCAGCTGTTCCAGCAACA 5850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AAGAAGCCCCTGGACTGCAATTCCGCTGAATGTGTGCCAAAGAAATCCAG 5900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CAGCCTAGAGACTGCGGAGCGGAAGAATCGACGGATGACGGCCTACGATG 5950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGAACCGTAAGCAGTTTTGCTGAAACGACTTACGTGACGGCGGAACTACG 6000 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACCGTTCCTTTGGTGGCAATTGCCACTGCGAGCAGCGGAGCTGCAAACAA 6050 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGACTGAGTTCGGACTGGAGGCAAAACGTTGAGTTCAACGATGATAAAAA 6100 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGATGATTAATGTTATCCAAGTATTAGGGATTTCTTTTGTTAGGTGTTAT 6150 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTTGCAAACGGTGGAATTCCACATCATAAAGTTTTCTATTTATATTTACG 6200 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCCTAAGTATTTGTCAAATACTATCAATACAAAAATTACAAAAACAATCG 6250 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TGATCGTAGTATTTAAATTTAAATCTGTATTATGCTGGTTTTTGTTTAAT 6300 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGTTAAATTCGATAAATAGTTAAATATTGTTAATTAAGAAATGTTTCCTT 6350 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CATCATGTGTAATAATATTTGGTTCATATGTATAGCATTGAATAAACTAT 6400 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TTACATTTTCTTAATGTTAAAATCAGATTCGCATAAGTTGCTGGTTCTAA 6450 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TAATAAAAGACATAAATTACTTAAATGTAAATCTAAATAAGCATAACGCT 6500 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F TCTTGTTTGAATTCCCGATTGCACCAATCGATAAGTCGACAGTAGCGCAA 6550 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ATACATATTCTAGTGTTGTGCATTGGAAAATTGACGGCCGCTTGAGTGTT 6600 *F CG16917|FBgn0032357|CT37528|FB *F \----------------------------GACGCAAAACTACGTAAAATTA 22 *F CG16917|FBgn0032357|CT37530|FB *F \----CGGCGTCAGTGTTGAATAGCTGCGGACGCAAAACTACGTAAAATTA 46 *F AF220354 *F GTGTCGGCGTCAGTGTTGAATAGCTGCGGACGCAAAACTACGTAAAATTA 6650 *F CG16917|FBgn0032357|CT37528|FB *F CGACAAAAAAACAGCGCCCCGCGAAAAAAGGCCATGAACTCGGCAATCAA 72 *F CG16917|FBgn0032357|CT37530|FB *F CGACAAAAAAACAGCGCCCCGCGAAAAAAGGCCATGAACTCGGCAATCAA 96 *F AF220354 *F CGACAAAAAAACAGCGCCCCGCGAAAAAAGGCCATGAACTCGGCAATCAA 6700 *F CG16917|FBgn0032357|CT37528|FB *F \-GGTGGGGGAGATATTCACGGCCGCCGGACAGGCGTTCAGCAGACTGGGC 121 *F CG16917|FBgn0032357|CT37530|FB *F \-GGTGGGGGAGATATTCACGGCCGCCGGACAGGCGTTCAGCAGACTGGGC 145 *F AF220354 *F AGGTGGGGGAGATATTCACGGCCGCCGGACAGGCGTTCAGCAGACTGGGC 6750 *F CG16917|FBgn0032357|CT37528|FB *F GATCTAACCATGCAGCTGCATCCCAATGCGGAGTCGCCATCGGGGAAGTG 171 *F CG16917|FBgn0032357|CT37530|FB *F GATCTAACCATGCAGCTGCATCCCAATGCGGAGTCGCCATCGGG------ 189 *F AF220354 *F GATCTAACCATGCAGCTGCATCCCAATGCGGAGTCGCCATCGGGGAAGTG 6800 *F CG16917|FBgn0032357|CT37528|FB *F GACGGACGAGGAGATCGACATGCTGCACTCGTCCATAATGCGCTTCTCCG 221 *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GACGGACGAGGAGATCGACATGCTGCACTCGTCCATAATGCGCTTCTCCG 6850 *F CG16917|FBgn0032357|CT37528|FB *F ACGACCTGACCAAGATCAGCCTAAGCATCAAGAACCGCACCGTTTCTCAG 271 *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------TTCTCAG 196 *F AF220354 *F ACGACCTGACCAAGATCAGCCTAAGCATCAAGAACCGCACCGTTTCTCAG 6900 *F CG16917|FBgn0032357|CT37528|FB *F ATCCGCCAGGCGCTGAAGAAGAAGGCCTTCGAGGACGCCGGCATTCCCGC 321 *F CG16917|FBgn0032357|CT37530|FB *F ATCCGCCAGGCGCTGAAGAAGAAGGCCTTCGAGGACGCCGGCATTCCCGC 246 *F AF220354 *F ATCCGCCAGGCGCTGAAGAAGAAGGCCTTCGAGGACGCCGGCATTCCCGC 6950 *F CG16917|FBgn0032357|CT37528|FB *F CAAGCAAGTGCCCGTCCAGCAGGTGCAACACGTTCTCCAGACGCTGCCAC 371 *F CG16917|FBgn0032357|CT37530|FB *F CAAGCAAGTGCCCGTCCAGCAGGTGCAACACGTTCTCCAGACGCTGCCAC 296 *F AF220354 *F CAAGCAAGTGCCCGTCCAGCAGGTGCAACACGTTCTCCAGACGCTGCCAC 7000 *F CG16917|FBgn0032357|CT37528|FB *F AACAGCAGCCGCAGCAGCCCCTTCAGCAGCCACCTCCTCAAGTATTCAAG 421 *F CG16917|FBgn0032357|CT37530|FB *F AACAGCAGCCGCAGCAGCCCCTTCAGCAGCCACCTCCTCAAGTATTCAAG 346 *F AF220354 *F AACAGCAGCCGCAGCAGCCCCTTCAGCAGCCACCTCCTCAAGTATTCAAG 7050 *F CG16917|FBgn0032357|CT37528|FB *F TCACAGCCAATCGTGCAGCACGTACAGCTGGTGGCGCAGCCAAAGCAGAT 471 *F CG16917|FBgn0032357|CT37530|FB *F TCACAGCCAATCGTGCAGCACGTACAGCTGGTGGCGCAGCCAAAGCAGAT 396 *F AF220354 *F TCACAGCCAATCGTGCAGCACGTACAGCTGGTGGCGCAGCCAAAGCAGAT 7100 *F CG16917|FBgn0032357|CT37528|FB *F CATCCTGCATCCACAGTCTAATACAACTACGACGGGCACGACGGTGACTG 521 *F CG16917|FBgn0032357|CT37530|FB *F CATCCTGCATCCACAGTCTAATACAACTACGACGGGCACGACGGTGACTG 446 *F AF220354 *F CATCCTGCATCCACAGTCTAATACAACTACGACGGGCACGACGGTGACTG 7150 *F CG16917|FBgn0032357|CT37528|FB *F TTAAGCAGTACCAGCAGATGCAGAAGGCTGCGGCATTAGCGGCAGCCCAG 571 *F CG16917|FBgn0032357|CT37530|FB *F TTAAGCAGTACCAGCAGATGCAGAAGGCTGCGGCATTAGCGGCAGCCCAG 496 *F AF220354 *F TTAAGCAGTACCAGCAGATGCAGAAGGCTGCGGCATTAGCGGCAGCCCAG 7200 *F CG16917|FBgn0032357|CT37528|FB *F GCTGCAGCCTCGACGGCCAACAACACGCCCACCATCACGGAGAACATCAT 621 *F CG16917|FBgn0032357|CT37530|FB *F GCTGCAGCCTCGACGGCCAACAACACGCCCACCATCACGGAGAACATCAT 546 *F AF220354 *F GCTGCAGCCTCGACGGCCAACAACACGCCCACCATCACGGAGAACATCAT 7250 *F CG16917|FBgn0032357|CT37528|FB *F TATCCAGCAGCCCACTTCGACTACGGCGACAGTGGTGCAGCAGCCACATA 671 *F CG16917|FBgn0032357|CT37530|FB *F TATCCAGCAGCCCACTTCGACTACGGCGACAGTGGTGCAGCAGCCACATA 596 *F AF220354 *F TATCCAGCAGCCCACTTCGACTACGGCGACAGTGGTGCAGCAGCCACATA 7300 *F CG16917|FBgn0032357|CT37528|FB *F TGGTGTCCACCTGCTCGTCGACGCAGATCGTGGTGCCCGTTGTGTCGGCA 721 *F CG16917|FBgn0032357|CT37530|FB *F TGGTGTCCACCTGCTCGTCGACGCAGATCGTGGTGCCCGTTGTGTCGGCA 646 *F AF220354 *F TGGTGTCCACCTGCTCGTCGACGCAGATCGTGGTGCCCGTTGTGTCGGCA 7350 *F CG16917|FBgn0032357|CT37528|FB *F GTGTCCACTGTTCCAACAGTACTCTCGCCCACGGTGGTGATTGCCGACGA 771 *F CG16917|FBgn0032357|CT37530|FB *F GTGTCCACTGTTCCAACAGTACTCTCGCCCACGGTGGTGATTGCCGACGA 696 *F AF220354 *F GTGTCCACTGTTCCAACAGTACTCTCGCCCACGGTGGTGATTGCCGACGA 7400 *F CG16917|FBgn0032357|CT37528|FB *F TGTGGTTAGCACCTCCAATCCTCCAGCGGCAGCGGCGACAGTAGGATCGC 821 *F CG16917|FBgn0032357|CT37530|FB *F TGTGGTTAGCACCTCCAATCCTCCAGCGGCAGCGGCGACAGTAGGATCGC 746 *F AF220354 *F TGTGGTTAGCACCTCCAATCCTCCAGCGGCAGCGGCGACAGTAGGATCGC 7450 *F CG16917|FBgn0032357|CT37528|FB *F CAGCAGGGGCGCCAGCAGGCTTAAAGTGCGGACCAGACGTGTCCATGACC 871 *F CG16917|FBgn0032357|CT37530|FB *F CAGCAGGGGCGCCAGCAGGCTTAAAGTGCGGACCAGACGTGTCCATGACC 796 *F AF220354 *F CAGCAGGGGCGCCAGCAGGCTTAAAGTGCGGACCAGACGTGTCCATGACC 7500 *F CG16917|FBgn0032357|CT37528|FB *F CTCAATCGCATCAATGTCCAGGAGAACGAGGTGGACGTGGAGGAGTGCCT 921 *F CG16917|FBgn0032357|CT37530|FB *F CTCAATCGCATCAATGTCCAGGAGAACGAGGTGGACGTGGAGGAGTGCCT 846 *F AF220354 *F CTCAATCGCATCAATGTCCAGGAGAACGAGGTGGACGTGGAGGAGTGCCT 7550 *F CG16917|FBgn0032357|CT37528|FB *F GCCCGCAGAGGTGGTCAAGCTGGATTTTGTCAGCGAGGAGGTGGCCGGGT 971 *F CG16917|FBgn0032357|CT37530|FB *F GCCCGCAGAGGTGGTCAAGCTGGATTTTGTCAGCGAGGAGGTGGCCGGGT 896 *F AF220354 *F GCCCGCAGAGGTGGTCAAGCTGGATTTTGTCAGCGAGGAGGTGGCCGGGT 7600 *F CG16917|FBgn0032357|CT37528|FB *F GA------------------------------------------------ 973 *F CG16917|FBgn0032357|CT37530|FB *F GA------------------------------------------------ 898 *F AF220354 *F GAGGTTCGAGCCCTCGGAACGGGCTGGACTTCTTCTACTAGATCTCCGGC 7650 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CGCAGAACGAATCACGTAACTTGGAACAGAACCGCTACAAATCCAGATCG 7700 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F AGAACTCTTAAGCAAAAAGGTTGAGATGCCGCCCTAGCCCTAAGTCTAAA 7750 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CTGTTAAGTGTTGTCATATTTGATATACCCTGAAATCACCTTGTTCCGGG 7800 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCCCGGATGACAAATGAATTGTGCGATGTGGAGGACGAGTCTGTGGATCG 7850 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F CCAGCCACAGAGCGTAGATATTAATTGCGGAGCGTATATGCGCCGCCTAG 7900 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F ACTTAAATTTACGCAAGACACAATAGTGCAAAGTACATAACGACGATCGT 7950 *F CG16917|FBgn0032357|CT37528|FB *F \-------------------------------------------------- *F CG16917|FBgn0032357|CT37530|FB *F \-------------------------------------------------- *F AF220354 *F GTAAGTTGAGAGTGAATAAACGAGCATTATTTTAAAAAAAAAAAAAAAAA 8000 *F CG16917|FBgn0032357|CT37528|FB \------ *F CG16917|FBgn0032357|CT37530|FB \------ *F AF220354 AAAAAA 8006 # *U FBrf0129107 *a Whitfield *b E. *t 2000.5.24 *T personal communication to FlyBase *u FlyBase error report for CG10199 on Wed May 24 08:20:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 24 16:16:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 24 May 2000 16:16:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 24 May 2000 08:20:09 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10199 on Wed May 24 08:20:08 2000 *F Content-Length: 903 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10199 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG10199. *F Comments: Celera has annotated 3 isoforms of l(3)82Fd: *F AE003603; AAF52045 *F AE003603; AAF52047 *F AE003603; AAF52048. *F A total of seven cDNAs, identifying seven different splice variants are *F identified by Stowers et al, Submitted FEB-1999: *F AF125384; AAD28508 *F AF125385; AAD28509 *F AF125386; AAD28510 *F AF125387; AAD28511 *F AF125388; AAD28512 *F AF125389; AAD28513 *F AF125390; AAD28514. *F Celera corresponds to 2 of these, so please add further CDS features to annotate *F the remaining 5. *F Also could you please recheck the splice variant identified as alt 3 as this *F splice variant has not be annotated by Stowers et al abd I wonder if it exists. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129108 *a Goldstein *b L. *t 2000.5.24 *T personal communication to FlyBase *u FlyBase error report for CG3339 on Wed May 24 16:28:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 25 00:23:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 May 2000 00:23:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 24 May 2000 16:28:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: LGOLDSTEIN@ucsd.edu *F Subject: FlyBase error report for CG3339 on Wed May 24 16:28:56 2000 *F Content-Length: 248 *F Error report from LARRY GOLDSTEIN (LGOLDSTEIN@UCSD.EDU) *F Gene or accession: CG3339 *F Missed gene *F Comments: TRANSLATION CLEARLY TRUNCATED OR ONLY A FRAGMENT *F Browser: Mozilla/4.51 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129109 *a Bayraktaroglu *b L. *t 2000.5.25 *T personal communication to FlyBase *u FlyBase error report for CG7528 on Thu May 25 10:16:04 2000. *F From leyla@morgan.harvard.edu Thu May 25 15:17:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 May 2000 15:17:36 \+0100 *F Date: Thu, 25 May 2000 10:16:04 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: merger of Uba2 and SAE2? *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Nest_of_Mice_707_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 27039 *F Hello Michael and the Cambridge curators, *F Michael, please take a look at the attached ClustalW and BLASTN *F alignments for Uba2 and SAE2. There are a few amino acid differences, *F but they could be due to sequencing 'differences' (errors and/or *F polymorphisms); I don't see evidence for a multigene family (BLAST *F hits only one region of one scaffold). *F What do you say? *F If you want to do a 3-way merger now, *F Uba2 FBgn0029113 = CG7528|FBgn0035840|CT23053| *F ClustalW of Uba2 and CG7528 is also attached. *F Leyla *F ================================================================================ *F Uba2 and SAE2 BLASTN alignments *F BLAST 2 SEQUENCES RESULTS VERSION BLASTN 2.0.11 Jan-20-2000 *F \------------------------------------------------------------------------ *F Sequence 1gi 6934295 Length 2328 (1 .. 2328) *F Sequence 2gi 6694273 Length 2352 (1 .. 2352) *F NOTE:The statistics (bitscore and expect value) is calculated based on the *F size of nr database *F Score = 4295 bits (2234), Expect = 0.0 *F Identities = 2282/2305 (99%), Positives = 2282/2305 (99%), Gaps = 2/2305 (0%) *F Query: 5 *F ggcggcgtcacttttctcggcttttccgtatcgcaattttgcaattttctcgccaaaaat 64 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 9 *F ggcggcgtcacttttctcggcttttccgtatcgcaattttgcaattttctcgccaaaaat 68 *F Query: 65 *F aagaaaaaggaaaacgtaaattcagaaaatggcagcagctatcaatggtgttttcccacc 124 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 69 *F aagaaaaaggaaaacgtaaattcagaaaatggcagcagctatcaatggtgttttcccacc 128 *F ubiquitin-like protein activati> 1 M A A A *F I N G V F P P *F Query: 125 *F cacattgcacgagctggtaaagaagtccaaggtgctggtcgtgggcgccggcggaatcgg 184 *F ||||||||| *F |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 129 *F cacattgcaggagctggtaaagaagtccaaggtgctggtcgtgggcgccggcggaatcgg 188 *F ubiquitin-like protein activati> 12 T L Q E L V K K S K V L V *F V G A G G I G *F Query: 185 *F ctgcgaggtgctcaaaaaccttgtgcttagcggctttacagacatcgaaattatcgatct 244 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 189 *F ctgcgaggtgctcaaaaaccttgtgcttagcggctttacagacatcgaaattatcgatct 248 *F ubiquitin-like protein activati> 32 C E V L K N L V L S G F T *F D I E I I D L *F Query: 245 *F ggacacaattgatcttagcaacttgaaccgacagttcctattccatcgcgagcacgttgg 304 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 249 *F ggacacaattgatcttagcaacttgaaccgacagttcctattccatcgcgagcacgttgg 308 *F ubiquitin-like protein activati> 52 D T I D L S N L N R Q F L *F F H R E H V G *F Query: 305 *F aaaatctaaggcacgggtggctagggaaagcgcactgagcttcaatccggacgctaagat 364 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 309 *F aaaatctaaggcacgggtggctagggaaagcgcactgagcttcaatccggacgctaagat 368 *F ubiquitin-like protein activati> 72 K S K A R V A R E S A L S *F F N P D A K I *F Query: 365 *F aaccgcttaccacgacagcgtaacatctactgattatggcgtcaacttctttaagaagtt 424 *F |||||||||||||||||| *F ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 369 *F aaccgcttaccacgacagtgtaacatctactgattatggcgtcaacttctttaagaagtt 428 *F ubiquitin-like protein activati> 92 T A Y H D S V T S T D Y G *F V N F F K K F *F Query: 425 *F cgatttggtgctcaacgccttggacaacagggccgacaggaatcatgtaaatcgcatgtg 484 *F |||||||||||||| |||||||||||||||||||| *F |||||||||||||||||||||||| *F Sbjct: 429 *F cgatttggtgctcagcgccttggacaacagggccgccaggaatcatgtaaatcgcatgtg 488 *F ubiquitin-like protein activati> 112 D L V L S A L D N R A A R *F N H V N R M C *F Query: 485 *F cctcaatgcggatgtcccgctgatcgagagcggcaccgccggctacaatggccaggtgga 544 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 489 *F cctcaatgcggatgtcccgctgatcgagagcggcaccgccggctacaatggccaggtgga 548 *F ubiquitin-like protein activati> 132 L N A D V P L I E S G T A *F G Y N G Q V E *F Query: 545 *F actgatcaagcgtggactcacccagtgctacgagtgcactccaaaagacaaacagcgaag 604 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 549 *F actgatcaagcgtggactcacccagtgctacgagtgcactccaaaagacaaacagcgaag 608 *F ubiquitin-like protein activati> 152 L I K R G L T Q C Y E C T *F P K D K Q R S *F Query: 605 *F cttcccgggctgcaccatacgcaatacgccctccgaaccgattcactgcattgtctgggc 664 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 609 *F cttcccgggctgcaccatacgcaatacgccctccgaaccgattcactgcattgtctgggc 668 *F ubiquitin-like protein activati> 172 F P G C T I R N T P S E P *F I H C I V W A *F Query: 665 *F caagcatctctttaatcaactgtttggtgagtccttggaagatgaggatatttctcctga 724 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 669 *F caagcatctctttaatcaactgtttggtgagtccttggaagatgaggatatttctcctga 728 *F ubiquitin-like protein activati> 192 K H L F N Q L F G E S L E *F D E D I S P D *F Query: 725 *F tgctgctgatcccgatgcaaaagaaaaagacggcggcgatggaaatggcgagcccaaagg 784 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 729 *F tgctgctgatcccgatgcaaaagaaaaagacggcggcgatggaaatggcgagcccaaagg 788 *F ubiquitin-like protein activati> 212 A A D P D A K E K D G G D *F G N G E P K G *F Query: 785 *F cgatgggaaggaaaaaggcgaagagtcaaaagaggaaaaggaggccaaggaggatactgc 844 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 789 *F cgatgggaaggaaaaaggcgaagagtcaaaagaggaaaaggaggccaaggaggatactgc 848 *F ubiquitin-like protein activati> 232 D G K E K G E E S K E E K *F E A K E D T A *F Query: 845 *F caacggcaacataatgcgcattaatactcgccaatgggccaaggactgcaactatgatgc 904 *F |||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||| *F Sbjct: 849 *F caacggcaacataatgcgcattaatactcgccaatgggccaaggacagcaactatgatgc 908 *F ubiquitin-like protein activati> 252 N G N I M R I N T R Q W A *F K D S N Y D A *F Query: 905 *F gggcaagctgttcaacaagttctttaacgaggacattacctatttgttgcgtatgtcgaa 964 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 909 *F gggcaagctgttcaacaagttctttaacgaggacattacctatttgttgcgtatgtcgaa 968 *F ubiquitin-like protein activati> 272 G K L F N K F F N E D I T *F Y L L R M S N *F Query: 965 *F tttgtggaagacccgcaaggcacccgtgcccgtgcagtgggataccctgctgcccgaagg 1024 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 969 *F tttgtggaagacccgcaaggcacccgtgcccgtgcagtgggataccctgctgcccgaagg 1028 *F ubiquitin-like protein activati> 292 L W K T R K A P V P V Q W *F D T L L P E G *F Query: 1025 *F atcctccggtgatcagaaggatgtggccaagcagcatcacaaggtgtggtccatcgagga 1084 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1029 *F atcctccggtgatcagaaggatgtggccaagcagcatcacaaggtgtggtccatcgagga 1088 *F ubiquitin-like protein activati> 312 S S G D Q K D V A K Q H H *F K V W S I E E *F Query: 1085 *F gtgcgctcaggtctttgccaattcattaaaagagttgagcgctaacttcctgaaactcga 1144 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1089 *F gtgcgctcaggtctttgccaattcattaaaagagttgagcgctaacttcctgaaactcga 1148 *F ubiquitin-like protein activati> 332 C A Q V F A N S L K E L S *F A N F L K L E *F Query: 1145 *F aggcgacgatactcttgcttgggacaaggacgaccagccagccatggatttcgtggcggc 1204 *F ||||||||||||||| *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1149 *F aggcgacgatactctggcttgggacaaggacgaccagccagccatggatttcgtggcggc 1208 *F ubiquitin-like protein activati> 352 G D D T L A W D K D D Q P *F A M D F V A A *F Query: 1205 *F ctgcgccaacgtgcgatcccacattttctacattgagccaaagtcaaggttcgagaataa 1264 *F |||||||||||||||||||||||||||| ||||||||| *F ||||||||||||||||| ||| *F Sbjct: 1209 *F ctgcgccaacgtgcgatcccacattttcgacattgagcgaaagtcaaggttcgagattaa 1268 *F ubiquitin-like protein activati> 372 C A N V R S H I F D I E R *F K S R F E I K *F Query: 1265 *F atcaatggcgggaaacattattcctgctattgccaccacaaatgcgattacggcgggaat 1324 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1269 *F gtcaatggcgggaaacattattcctgctattgccaccacaaatgcgattacggcgggaat 1328 *F ubiquitin-like protein activati> 392 S M A G N I I P A I A T T *F N A I T A G I *F Query: 1325 *F ttccgtgatgcgggctttcaaagtgctggaggccaagtgggagcagtgccaggccgtcta 1384 *F ||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 1329 *F ttccgtgatgcgggctttcaaagtgctggaggccaagtgggagcagtgcaaggccgtcta 1388 *F ubiquitin-like protein activati> 412 S V M R A F K V L E A K W *F E Q C K A V Y *F Query: 1385 *F tgcccgacttagaccaaatgcacgaaatcacttccttgtaccggacgcttctcttcctgg 1444 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1389 *F tgcccgacttagaccaaatgcacgaaatcacttccttgtaccggacgcttctcttcctgg 1448 *F ubiquitin-like protein activati> 432 A R L R P N A R N H F L V *F P D A S L P G *F Query: 1445 *F ccccaatcccaattgccatgtatgcgccagcgatccggccattactctcaagatcgatac 1504 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1449 *F ccccaatcccaattgccatgtatgcgccagcgatccggccattactctcaagatcgatac 1508 *F ubiquitin-like protein activati> 452 P N P N C H V C A S D P A *F I T L K I D T *F Query: 1505 *F gaagcgcatgcgtataaaggagctgcgtgacgaggtcctggttaagacgctcaacatgtt 1564 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1509 *F gaagcgcatgcgtataaaggagctgcgtgacgaggtcctggttaagacgctcaacatgtt 1568 *F ubiquitin-like protein activati> 472 K R M R I K E L R D E V L *F V K T L N M L *F Query: 1565 *F gaatccggatgtgactgtgcagagcaatggctccattttaatctcctcagaggagggcga 1624 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1569 *F gaatccggatgtgactgtgcagagcaatggctccattttaatctcctcagaggagggcga 1628 *F ubiquitin-like protein activati> 492 N P D V T V Q S N G S I L *F I S S E E G E *F Query: 1625 *F gaccgaatgcaatgacggcaagctcctaagcgaattaaacattgtggatggtgtgatcct 1684 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1629 *F gaccgaatgcaatgacggcaagctcctaagcgaattaaacattgtggatggtgtgatcct 1688 *F ubiquitin-like protein activati> 512 T E C N D G K L L S E L N *F I V D G V I L *F Query: 1685 *F caagtgcgacgacttcttccagaactacgagttgagtattatcatttcccacttcgatgc 1744 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1689 *F caagtgcgacgacttcttccagaactacgagttgagtattatcatttcccacttcgatgc 1748 *F ubiquitin-like protein activati> 532 K C D D F F Q N Y E L S I *F I I S H F D A *F Query: 1745 *F ggagcgcgatgaaaacttgttcgaagtcgtagcagatgcctcacagctgaagccaaagga 1804 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1749 *F ggagcgcgatgaaaacttgttcgaagtcgtagcagatgcctcacagctgaagccaaagga 1808 *F ubiquitin-like protein activati> 552 E R D E N L F E V V A D A *F S Q L K P K D *F Query: 1805 *F tgaggatcagaaggaggccgtgaaagacaaggaggatgaaccgaaatcggctaagaagcg 1864 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1809 *F tgaggatcagaaggaggccgtgaaagacaaggaggatgaaccgaaatcggctaagaagcg 1868 *F ubiquitin-like protein activati> 572 E D Q K E A V K D K E D E *F P K S A K K R *F Query: 1865 *F ttctaccaacggggaaggagactcaaaggacgatggaccctccacttcgaagcgcagccg 1924 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1869 *F ttctaccaacggggaaggagactcaaaggacgatggaccctccacttcgaagcgcagccg 1928 *F ubiquitin-like protein activati> 592 S T N G E G D S K D D G P *F S T S K R S R *F Query: 1925 *F gcccaacgaagtggttgaggaagatgacgatgactgtctggtaatcgaggaagacgaaga 1984 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1929 *F gcccaacgaagtggttgaggaagatgacgatgactgtctggtaatcgaggaagacgaaga 1988 *F ubiquitin-like protein activati> 612 P N E V V E E D D D D C L *F V I E E D E D *F Query: 1985 *F ccaagcagatgttgttgtcgtggccacagacaagctctctgtgcagagtcccccaaaatc 2044 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1989 *F ccaagcagatgttgttgtcgtggccacagacaagctctctgtgcagagtcccccaaaatc 2048 *F ubiquitin-like protein activati> 632 Q A D V V V V A T D K L S *F V Q S P P K S *F Query: 2045 *F gggctccaagcgcaagccatgtgaagtaatcgaggatgaggatatcaccgagattttgga 2104 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2049 *F gggctccaagcgcaagccatgtgaagtaatcgaggatgaggatatcaccgagattttgga 2108 *F ubiquitin-like protein activati> 652 G S K R K P C E V I E D E *F D I T E I L E *F Query: 2105 *F gtcgtctgacgacgaacccgcgggaccaaccaagtgcaaacgttcccgcctggacgattc 2164 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2109 *F gtcgtctgacgacgaacccgcgggaccaaccaagtgcaaacgttcccgcctggacgattc 2168 *F ubiquitin-like protein activati> 672 S S D D E P A G P T K C K *F R S R L D D S *F Query: 2165 *F gaaccccgtggcagtcatcagtatcgattaatnnnnnnnngaagtttttattcgaataag 2224 *F |||||||||||||||||||||||||||||||| *F || ||||||||||||||||| *F Sbjct: 2169 *F gaaccccgtggcagtcatcagtatcgattaataaaaaaaagatgtttttattcgaataag 2228 *F ubiquitin-like protein activati> 692 N P V A V I S I D ^^^ *F Query: 2225 *F ttctcaactgcgcactaaattatgctcttgattccttgtaatttcattcttgatttaaaa 2284 *F ||||||||||||||||||||||||||||||||||| *F ||||||||||||| |||||||||| *F Sbjct: 2229 *F ttctcaactgcgcactaaattatgctcttgattccctgtaatttcattc-tgatttaaaa 2287 *F Query: 2285 taaaaacacattttttgtggatgat 2309 *F |||||||||| |||||||||||||| *F Sbjct: 2288 taaaaacaca-tttttgtggatgat 2311 *F CPU time: 0.11 user secs. 0.06 sys. secs 0.17 total secs. Gapped Lambda K H *F 1.33 0.621 1.12 Matrix: blastn matrix:1 \-2 Gap Penalties: Existence: 5, *F Extension: 2 Number of Hits to DB: 14 Number of Sequences: 0 Number of *F extensions: 14 Number of successful extensions: 3 Number of sequences better *F than 10.0: 1 length of query: 2328 length of database: 706,884,452 effective *F HSP length: 24 effective length of query: 2304 effective length of database: *F 699,596,996 effective search space: 1611871478784 effective search space *F used: 1611871478784 T: 0 A: 0 X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 1 *F ================================================================================ *F SAE2 Uba2 CLUSTALW alignment *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|6694274|gb|AAF25197.1|AF193 700 aa *F Sequence 2: gi|6934296|gb|AAF31704.1|AF218 700 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 98 *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:11481 *F Alignment Score 4265 *F CLUSTAL W (1.8) multiple sequence alignment *F gi|6694274|gb|AAF25197.1|AF193 *F MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50 *F gi|6934296|gb|AAF31704.1|AF218 *F MAAAINGVFPPTLHELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50 *F gi|6694274|gb|AAF25197.1|AF193 *F LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100 *F gi|6934296|gb|AAF31704.1|AF218 *F LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100 *F gi|6694274|gb|AAF25197.1|AF193 *F TDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQV 150 *F gi|6934296|gb|AAF31704.1|AF218 *F TDYGVNFFKKFDLVLNALDNRADRNHVNRMCLNADVPLIESGTAGYNGQV 150 *F gi|6694274|gb|AAF25197.1|AF193 *F ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200 *F gi|6934296|gb|AAF31704.1|AF218 *F ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200 *F gi|6694274|gb|AAF25197.1|AF193 *F ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250 *F gi|6934296|gb|AAF31704.1|AF218 *F ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250 *F gi|6694274|gb|AAF25197.1|AF193 *F ANGNIMRINTRQWAKDSNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300 *F gi|6934296|gb|AAF31704.1|AF218 *F ANGNIMRINTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300 *F gi|6694274|gb|AAF25197.1|AF193 *F PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350 *F gi|6934296|gb|AAF31704.1|AF218 *F PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350 *F gi|6694274|gb|AAF25197.1|AF193 *F EGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPA 400 *F gi|6934296|gb|AAF31704.1|AF218 *F EGDDTLAWDKDDQPAMDFVAACANVRSHIFYIEPKSRFENKSMAGNIIPA 400 *F gi|6694274|gb|AAF25197.1|AF193 *F IATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLP 450 *F gi|6934296|gb|AAF31704.1|AF218 *F IATTNAITAGISVMRAFKVLEAKWEQCQAVYARLRPNARNHFLVPDASLP 450 *F gi|6694274|gb|AAF25197.1|AF193 *F GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500 *F gi|6934296|gb|AAF31704.1|AF218 *F GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500 *F gi|6694274|gb|AAF25197.1|AF193 *F GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550 *F gi|6934296|gb|AAF31704.1|AF218 *F GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550 *F gi|6694274|gb|AAF25197.1|AF193 *F AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600 *F gi|6934296|gb|AAF31704.1|AF218 *F AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600 *F gi|6694274|gb|AAF25197.1|AF193 *F DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650 *F gi|6934296|gb|AAF31704.1|AF218 *F DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650 *F gi|6694274|gb|AAF25197.1|AF193 *F SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID 700 *F gi|6934296|gb|AAF31704.1|AF218 *F SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID 700 *F ================================================================================ *F Uba2 FBgn0029113= CG7528|FBgn0035840|CT23053| *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: CG7528|FBgn0035840|CT23053|FBa 823 aa *F Sequence 2: gi|6694274|gb|AAF25197.1|AF193 700 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 99 *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:11503 *F Alignment Score 4275 *F CLUSTAL W (1.8) multiple sequence alignment *F CG7528|FBgn0035840|CT23053|FBa *F MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50 *F gi|6694274|gb|AAF25197.1|AF193 *F MAAAINGVFPPTLQELVKKSKVLVVGAGGIGCEVLKNLVLSGFTDIEIID 50 *F CG7528|FBgn0035840|CT23053|FBa *F LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100 *F gi|6694274|gb|AAF25197.1|AF193 *F LDTIDLSNLNRQFLFHREHVGKSKARVARESALSFNPDAKITAYHDSVTS 100 *F CG7528|FBgn0035840|CT23053|FBa *F TDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQV 150 *F gi|6694274|gb|AAF25197.1|AF193 *F TDYGVNFFKKFDLVLSALDNRAARNHVNRMCLNADVPLIESGTAGYNGQV 150 *F CG7528|FBgn0035840|CT23053|FBa *F ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200 *F gi|6694274|gb|AAF25197.1|AF193 *F ELIKRGLTQCYECTPKDKQRSFPGCTIRNTPSEPIHCIVWAKHLFNQLFG 200 *F CG7528|FBgn0035840|CT23053|FBa *F ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250 *F gi|6694274|gb|AAF25197.1|AF193 *F ESLEDEDISPDAADPDAKEKDGGDGNGEPKGDGKEKGEESKEEKEAKEDT 250 *F CG7528|FBgn0035840|CT23053|FBa *F ANGNIMRINTRQWAKDCNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300 *F gi|6694274|gb|AAF25197.1|AF193 *F ANGNIMRINTRQWAKDSNYDAGKLFNKFFNEDITYLLRMSNLWKTRKAPV 300 *F CG7528|FBgn0035840|CT23053|FBa *F PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350 *F gi|6694274|gb|AAF25197.1|AF193 *F PVQWDTLLPEGSSGDQKDVAKQHHKVWSIEECAQVFANSLKELSANFLKL 350 *F CG7528|FBgn0035840|CT23053|FBa *F EGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPA 400 *F gi|6694274|gb|AAF25197.1|AF193 *F EGDDTLAWDKDDQPAMDFVAACANVRSHIFDIERKSRFEIKSMAGNIIPA 400 *F CG7528|FBgn0035840|CT23053|FBa *F IATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLP 450 *F gi|6694274|gb|AAF25197.1|AF193 *F IATTNAITAGISVMRAFKVLEAKWEQCKAVYARLRPNARNHFLVPDASLP 450 *F CG7528|FBgn0035840|CT23053|FBa *F GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500 *F gi|6694274|gb|AAF25197.1|AF193 *F GPNPNCHVCASDPAITLKIDTKRMRIKELRDEVLVKTLNMLNPDVTVQSN 500 *F CG7528|FBgn0035840|CT23053|FBa *F GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550 *F gi|6694274|gb|AAF25197.1|AF193 *F GSILISSEEGETECNDGKLLSELNIVDGVILKCDDFFQNYELSIIISHFD 550 *F CG7528|FBgn0035840|CT23053|FBa *F AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600 *F gi|6694274|gb|AAF25197.1|AF193 *F AERDENLFEVVADASQLKPKDEDQKEAVKDKEDEPKSAKKRSTNGEGDSK 600 *F CG7528|FBgn0035840|CT23053|FBa *F DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650 *F gi|6694274|gb|AAF25197.1|AF193 *F DDGPSTSKRSRPNEVVEEDDDDCLVIEEDEDQADVVVVATDKLSVQSPPK 650 *F CG7528|FBgn0035840|CT23053|FBa *F SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAMSLAS 700 *F gi|6694274|gb|AAF25197.1|AF193 *F SGSKRKPCEVIEDEDITEILESSDDEPAGPTKCKRSRLDDSNPVAVISID 700 *F \*********************************************: . *F CG7528|FBgn0035840|CT23053|FBa *F RILQQGSRLWNGLSAARGFHLLTRPAAPAIVSIQSSQLVAATTGICQTSG 750 *F gi|6694274|gb|AAF25197.1|AF193 *F \-------------------------------------------------- *F CG7528|FBgn0035840|CT23053|FBa *F LLTPGSTLVQQVAGFKVKGRLKRRCKDCYIVVRQERGYVICPTHPRHKQM 800 *F gi|6694274|gb|AAF25197.1|AF193 *F \-------------------------------------------------- *F CG7528|FBgn0035840|CT23053|FBa SMKKRDYKSWILTHATQSKERGY 823 *F gi|6694274|gb|AAF25197.1|AF193 \----------------------- *F ================================================================================ # *U FBrf0129110 *a Boehm *b S. *t 2000.5.25 *T personal communication to FlyBase *u FlyBase error report for CG12299 on Thu May 25 09:56:07 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 25 17:51:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 May 2000 17:51:07 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 25 May 2000 09:56:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: boehm@mdc-berlin.de *F Subject: FlyBase error report for CG12299 on Thu May 25 09:56:07 2000 *F Content-Length: 1042 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG12299 *F Gene annotation error *F Gene CG12299 has incorrect exon/intron structure. *F Comments: Hi *F 1.the CG12299 translation contains 167aa and no C2H2 zinc finger motif in *F contrast to the 10 annotated fingers (1isolated and 9 tandem fingers) *F 2.obviously this is a fragment derived from a CDS(504bp) translated in frame+1 *F in pos.230725..231228 of AE003629 *F 3. the mRNA with 3305bp (pos.230034..230454,230563..233446)contains an ORF in *F frame+2 (pos.231134..232783) with 550aa containing 9 tandem fingers in large *F agreement with the annotation *F 4. The CDS (frame+1) and my proposed ORF (frame+2) overlap and have to be *F joint in some manner,but I am not an expert to create a correct gene prediction *F Please have a look to this gene annotation.It will be fine if you can inform *F me about your success in a corrected prediction. *F Best wishes Siegfried Boehm (MDC,Berlin) *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 95) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129111 *a Silverstein *b A.M. *t 2000.5.25 *T personal communication to FlyBase *u FlyBase error report for CG8402 on Thu May 25 16:09:59 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri May 26 00:04:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 26 May 2000 00:04:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 25 May 2000 16:09:59 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: adam.silverstein@email.swmed.edu *F Subject: FlyBase error report for CG8402 on Thu May 25 16:09:59 2000 *F Content-Length: 489 *F Error report from Adam M. Silverstein (adam.silverstein@email.swmed.edu) *F Gene or accession: CG8402 *F Missed gene *F Comments: This gene is the Drosophila homolog of mammalian Protein Phosphatase *F 5 and yeast PPT. To date, it is the only known phosphatase with an N-terminal *F tetratricopeptide repeat(TPR)domain. The Amino acid sequence is 57% *F identical to human and rat PP5. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows NT; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129112 *a Bryant *b P. *t 2000.5.26 *T personal communication to FlyBase *u FlyBase error report for CG5462 on Fri May 26 09:22:48 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri May 26 17:17:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 26 May 2000 17:17:46 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 26 May 2000 09:22:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: pjbryant@uci.edu *F Subject: FlyBase error report for CG5462 on Fri May 26 09:22:48 2000 *F Content-Length: 362 *F Error report from Peter Bryant (pjbryant@uci.edu) *F Gene or accession: CG5462 *F Gene annotation error *F Gene CG5462 corresponds to AJ252084 (FBgn0028975) *F Comments: Reported under the names Vartul (FBgn0028975 AJ252084) and *F Scribble(d) (FBgn0026178 AF190774) *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 98) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From FlyBase-error@hedgehog.lbl.gov Fri May 26 17:18:25 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 26 May 2000 17:18:25 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 26 May 2000 09:23:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: pjbryant@uci.edu *F Subject: FlyBase error report for CG5462 on Fri May 26 09:23:35 2000 *F Content-Length: 363 *F Error report from Peter Bryant (pjbryant@uci.edu) *F Gene or accession: CG5462 *F Gene annotation error *F Gene CG5462 corresponds to AF190774 (FBgn0026178 ) *F Comments: Reported under the names Vartul (FBgn0028975 AJ252084) and *F Scribble(d) (FBgn0026178 AF190774) *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 98) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129113 *a Mohr *b S.C. *t 2000.5.26 *T personal communication to FlyBase *u FlyBase error report for CG7842 on Fri May 26 19:28:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat May 27 03:24:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 27 May 2000 03:24:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 26 May 2000 19:28:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mohr@darwin.bu.edu *F Subject: FlyBase error report for CG7842 on Fri May 26 19:28:55 2000 *F Content-Length: 1065 *F Error report from Scott C. Mohr (mohr@darwin.bu.edu) *F Gene or accession: CG7842 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG7842. *F Comments: This is a CORRECTION to an earlier annotation that I submitted about *F 2 hours ago. I mistakenly submitted a new annotation for CT23794 on a form *F for CG6650; it should have been submitted on this form, of course, since *F CT23794 derives from CG7842. So, I will repeat my early contribution, viz., ' *F On the basis of its match to our sequence profile for YOR221C (S. cerevisiae, *F mitochondrial malonyl-CoA: ACP transacylase) and its position on a *F bootstrapped maximum *F parsimony phylogenetic tree we can confidently predict that CT23794 shares the *F function *F and subcellular location of YOR221C. It shares a branch with C. elegans gene *F C50D2_7 *F which we can similarly annotate as a third ortholog. I sincerely apologize *F for any confusion this mistake may have caused. Scott Mohr *F Browser: Mozilla/4.73 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129114 *a Crabtree *b G.R. *t 2000.5.27 *T personal communication to FlyBase *u FlyBase error report for CG11172 on Sat May 27 16:58:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun May 28 00:52:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 28 May 2000 00:52:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 27 May 2000 16:58:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: crabtree@cmgm.stanford.edu *F Subject: FlyBase error report for CG11172 on Sat May 27 16:58:10 2000 *F Content-Length: 859 *F Error report from Gerald R. Crabtree (crabtree@cmgm.stanford.edu) *F Gene or accession: CG11172 *F Missed gene *F Comments: The gene CG11172 is most related to human TonEBP (AAD18136), a *F transcription factor that mediates the activation of a large group of genes *F that controls the response to hypertonicity 4 : Rim JS, Atta MG, Dahl SC, *F Berry GT, Handler JS, Kwon HM. J Biol Chem. 1998 Aug 7;273(32):20615-21. *F You have mistakenly said that it is related to the NF-ATc family of *F transcription factors which have a role in growth and development and hence *F put it in the wrong category of genes. In fact CG11172 does not have the *F structural regions in the NF-ATc family that mediate its import and export *F from the nucleus and which distinquishes it from the TonEBP family. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129115 *a Boehm *b S. *t 2000.5.29 *T personal communication to FlyBase *u FlyBase error report for CG5086 on Mon May 29 05:46:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 29 13:41:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 29 May 2000 13:41:54 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 29 May 2000 05:46:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: boehm@mdc-berlin.de *F Subject: FlyBase error report for CG5086 on Mon May 29 05:46:54 2000 *F Content-Length: 906 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG5086 *F Gene annotation error *F Gene CG5086 has incorrect exon/intron structure. *F Comments: 1.the CG5086 translation has 517aa and no C2H2 zinc fingers as shown *F in the GadFly annotation (6 tandem fingers) *F 2.my translation in frame \-2 of AE003433 pos.54670 to 57479 (treated as one *F exon)results in a new ORF with 936aa containing 7 tandem fingers *F 3.therefore it seems to me that the two exons shown in the annotation have to *F be joint.The N-terminal part of the new ORF is (from aa 1-509) identical to the *F annotated translation.I am not sure about the true C-terminus of the proposed *F ORF. *F I would be glad about your reply to my proposal as in the case of my recent *F error report on CG12299. *F Cheers Siegfried Boehm (MDC,Berlin) *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 95) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129117 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG6727 on Tue May 16 08:19:59 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 16:15:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 16:15:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 08:19:59 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6727 on Tue May 16 08:19:59 2000 *F Content-Length: 370 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6727 *F Gene annotation error *F Genes gom and CG6727 should be merged. *F Comments: Sequence is identical for gom (AF045470; AAF18568) and Celera *F CG6727 (AE003456; AAF46808), please merge the genes. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129118 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG6727 on Tue May 16 08:31:44 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 16:26:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 16:26:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 08:31:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6727 on Tue May 16 08:31:44 2000 *F Content-Length: 936 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6727 *F Gene annotation error *F Gene gom has incorrect exon/intron structure. *F Comments: The submitted sequence for gom cDNA (AF045470; AAF18568) is longer *F than the *F Celera gene CG6727 (AE003456; AAF46808) by N-terminal 20 amino acids. *F These upstream amino acids can be found in the Celera sequence using *F the upstream exon as defined by the mRNA feature. *F Please amend the CDS feature to show the longest possible ORF. *F FT CDS join(148538..148853,148903..149444) *F > *F FT CDS join(148410..148455,148524..148853,148903..149444) *F Also, annotation of the nucleotide entry shows the gene is Fragile-X-related *F (FBgn0028734) but it is not as this is on the third chromosome. Please update *F the annotation to remove this gene and replace with gom. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129119 *a Whitfield *b E. *t 2000.5.18 *T personal communication to FlyBase *u FlyBase error report for CG17131 on Thu May 18 06:39:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 18 14:35:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 May 2000 14:35:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 18 May 2000 06:39:51 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17131 on Thu May 18 06:39:50 2000 *F Content-Length: 417 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17131 *F Gene annotation error *F Genes CG17131 and SP71 should be merged. *F Comments: Serano et al submitted sequence AF212322; AAF24495 of SP71 whose *F translation aligns 100% with Celera CG17131 sequence AE002567; AAF45388. *F Please merge the genes. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129120 *a Bayraktaroglu *b L. *t 2000.5.22 *T personal communication to FlyBase *u FlyBase error report for CG12276 on Mon May 22 13:44:29 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 22 21:41:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 22 May 2000 21:41:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 22 May 2000 13:44:29 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F Subject: FlyBase error report for CG12276 on Mon May 22 13:44:29 2000 *F Content-Length: 450 *F Error report from Leyla Bayraktaroglu (leyla@morgan.harvard.edu) *F Gene or accession: CG12276 *F Gene annotation error *F Gene CG12276 corresponds to AF193554 (FBgn0029512) *F Comments: CG12276|CT17770| corresponds to Aos1 (FBgn0029512). ClustalW *F alignments of *F the Aos1 and CG12276|CT17770| CDSs are being sent to flybase-updates in a *F separate message. *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From leyla@morgan.harvard.edu Mon May 22 21:43:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 22 May 2000 21:43:57 \+0100 *F Date: Mon, 22 May 2000 16:42:16 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: Aos1-CG12276|CT17770 ClustalW alignment *F To: flybase-updates@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-MD5: 51kkpUi+Le+Ioj2WuS9aeg== *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Aos1 corresponds to CG12276. *F Aos1 and CG12276|CT17770 ClustalW alignment. *F Pairwise Scores: *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|6694276|gb|AAF25198.1|AF193 337 aa *F Sequence 2: gi|7299631|gb|AAF54815.1| 337 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 99 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/1047959028373.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:5488 *F Alignment Score 2029 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/1047959028373.aln *F CLUSTAL W (1.8) multiple sequence alignment *F gi|6694276|gb|AAF25198.1|AF193 *F MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50 *F gi|7299631|gb|AAF54815.1| *F MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50 *F gi|6694276|gb|AAF25198.1|AF193 *F GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100 *F gi|7299631|gb|AAF54815.1| *F GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100 *F gi|6694276|gb|AAF25198.1|AF193 *F RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150 *F gi|7299631|gb|AAF54815.1| *F RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150 *F gi|6694276|gb|AAF25198.1|AF193 *F DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVS 200 *F gi|7299631|gb|AAF54815.1| *F DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVS 200 *F gi|6694276|gb|AAF25198.1|AF193 *F IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250 *F gi|7299631|gb|AAF54815.1| *F IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250 *F gi|6694276|gb|AAF25198.1|AF193 *F RDPSYKTREADLELLRGIRDELLPNSILGNEALGLIFAQISPAVAVVGGV 300 *F gi|7299631|gb|AAF54815.1| *F RDPSYKTREADLELLRGIRDELLPNSILGDEALGLIFAQISPAVAVVGGV 300 *F gi|6694276|gb|AAF25198.1|AF193 VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337 *F gi|7299631|gb|AAF54815.1| VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337 *F From leyla@morgan.harvard.edu Mon May 22 21:45:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 22 May 2000 21:45:27 \+0100 *F Date: Mon, 22 May 2000 16:43:59 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: Aos1 and SAE1 (plus CG12276) *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Rookery_of_Penguins_044_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 13678 *F Michael and the Cambridge curators, *F By alignment, Aos1 and SAE1 seem to be the same gene. *F The attached file contains the BLASTN and ClustalW alignments *F for the nucleotide and protein sequences for Aos1 and SAE1. *F I have just sent in a FB-updates/error report indicating *F that Aos1 corresponds to CG12276|CT17770. *F Leyla *F ================================================================================ *F SAE1 AF218863 AAF31703.1 *F Aos1 AF193554 AAF25198.1 *F SAE1 and Aos1 BLASTN alignment *F Sequence 1 gi 6934293 Length 1169 (1 .. 1169) (SAE1) *F Sequence 2 gi 6694275Length 1169 (1 .. 1169) (Aos1) *F Score = 2173 bits (1130), Expect = 0.0 *F Identities = 1134/1136 (99%), Positives = 1134/1136 (99%) *F Query: 4 *F ctgaaagttgttgcaagaaatttgtattaagttgtgaaaaatacattttaaacttgataa 63 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 9 *F ctgaaagttgttgcaagaaatttgtattaagttgtgaaaaatacattttaaacttgataa 68 *F Query: 64 *F ataaataatggtcgtggatatggacaccagcgagacagccgtggagctcactgaggcgga 123 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 69 *F ataaataatggtcgtggatatggacaccagcgagacagccgtggagctcactgaggcgga 128 *F ubiquitin-like protein activati> 1 M V V D M D T S E T A *F V E L T E A E *F Query: 124 *F gaacgagctgtacgacagacaaatccgactctggggtctggaatctcagaaacgccttcg 183 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 129 *F gaacgagctgtacgacagacaaatccgactctggggtctggaatctcagaaacgccttcg 188 *F ubiquitin-like protein activati> 19 N E L Y D R Q I R L W G L *F E S Q K R L R *F Query: 184 *F cacggccaagattctcatcgctggactgtgcggcctgggcgccgagataaccaagaacat 243 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 189 *F cacggccaagattctcatcgctggactgtgcggcctgggcgccgagataaccaagaacat 248 *F ubiquitin-like protein activati> 39 T A K I L I A G L C G L G *F A E I T K N I *F Query: 244 *F catcctgtccggagtgaactccgtgaagctgctggatgacaaggacgtaaccgaggagga 303 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 249 *F catcctgtccggagtgaactccgtgaagctgctggatgacaaggacgtaaccgaggagga 308 *F ubiquitin-like protein activati> 59 I L S G V N S V K L L D D *F K D V T E E D *F Query: 304 *F cttttgttcacaattccttgtcccccgtgaatcgctgaacaccaaccgggccgaagcatc 363 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 309 *F cttttgttcacaattccttgtcccccgtgaatcgctgaacaccaaccgggccgaagcatc 368 *F ubiquitin-like protein activati> 79 F C S Q F L V P R E S L N *F T N R A E A S *F Query: 364 *F attgacacgggcacgtgctctcaatcccatggtggacatctccgccgaccgcgagccctt 423 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 369 *F attgacacgggcacgtgctctcaatcccatggtggacatctccgccgaccgcgagccctt 428 *F ubiquitin-like protein activati> 99 L T R A R A L N P M V D I *F S A D R E P L *F Query: 424 *F gaaggagaagacctctgagttcttcggtcagttcgacgttgtggtggtcaatggcgcgac 483 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 429 *F gaaggagaagacctctgagttcttcggtcagttcgacgttgtggtggtcaatggcgcgac 488 *F ubiquitin-like protein activati> 119 K E K T S E F F G Q F D V *F V V V N G A T *F Query: 484 *F caacgaggaactgttgcgcattgacaccatttgccgggacctgggcgtcaagttcatagc 543 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 489 *F caacgaggaactgttgcgcattgacaccatttgccgggacctgggcgtcaagttcatagc 548 *F ubiquitin-like protein activati> 139 N E E L L R I D T I C R D *F L G V K F I A *F Query: 544 *F caccgatgtgtggggcaccttcgggttctactttgccagtctgcagaagcacagctacgt 603 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 549 *F caccgatgtgtggggcaccttcgggttctactttgccagtctgcagaagcacagctacgt 608 *F ubiquitin-like protein activati> 159 T D V W G T F G F Y F A S *F L Q K H S Y V *F Query: 604 *F cgaggatgttattaatcacaaggtcgtagccaattcggagaagaaaaagaagtatgaaac 663 *F ||||||||||||||| *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 609 *F cgaggatgttattaagcacaaggtcgtagccaattcggagaagaaaaagaagtatgaaac 668 *F ubiquitin-like protein activati> 179 E D V I K H K V V A N S E *F K K K K Y E T *F Query: 664 *F cgtgtccattccaacgcagcgtgatgtggattatcccggctactctgcctggcttgattt 723 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 669 *F cgtgtccattccaacgcagcgtgatgtggattatcccggctactctgcctggcttgattt 728 *F ubiquitin-like protein activati> 199 V S I P T Q R D V D Y P G *F Y S A W L D F *F Query: 724 *F cgatgttaccgagcctagttatttgcgaaaattgaagcgcaatggcccgggtgttttgct 783 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 729 *F cgatgttaccgagcctagttatttgcgaaaattgaagcgcaatggcccgggtgttttgct 788 *F ubiquitin-like protein activati> 219 D V T E P S Y L R K L K R *F N G P G V L L *F Query: 784 *F attaagtgtcctgcaaaagttccgcacaacccacaagcgggatcccagctacaagacccg 843 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 789 *F attaagtgtcctgcaaaagttccgcacaacccacaagcgggatcccagctacaagacccg 848 *F ubiquitin-like protein activati> 239 L S V L Q K F R T T H K R *F D P S Y K T R *F Query: 844 *F agaagctgacctggaattgcttcgcggaattcgcgacgaactgctgcccaactctatact 903 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 849 *F agaagctgacctggaattgcttcgcggaattcgcgacgaactgctgcccaactctatact 908 *F ubiquitin-like protein activati> 259 E A D L E L L R G I R D E *F L L P N S I L *F Query: 904 *F gggcgacgaagctctaggtctgatttttgcacagatctcgccagcggtcgctgttgtcgg 963 *F |||| *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 909 *F gggcaacgaagctctaggtctgatttttgcacagatctcgccagcggtcgctgttgtcgg 968 *F ubiquitin-like protein activati> 279 G N E A L G L I F A Q I S *F P A V A V V G *F Query: 964 *F cggcgttgtggcacaagaggtcatcaaggtggttactaaattggaggcgccacaccgtaa 1023 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 969 *F cggcgttgtggcacaagaggtcatcaaggtggttactaaattggaggcgccacaccgtaa 1028 *F ubiquitin-like protein activati> 299 G V V A Q E V I K V V T K *F L E A P H R N *F Query: 1024 *F tctgttcgtttttgacccagagacttgtgccggatacgttgaggccatcggagcgaagtg 1083 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1029 *F tctgttcgtttttgacccagagacttgtgccggatacgttgaggccatcggagcgaagtg 1088 *F ubiquitin-like protein activati> 319 L F V F D P E T C A G Y V *F E A I G A K ^^ *F Query: 1084 *F aatttaccacaaagggcttatttataaaaatctgaatatcaaataacaccagttgt 1139 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1089 *F aatttaccacaaagggcttatttataaaaatctgaatatcaaataacaccagttgt 1144 *F ubiquitin-like protein activati> 338 ^ *F CPU time: 0.11 user secs. 0.02 sys. secs 0.13 total secs. Gapped Lambda K H *F 1.33 0.621 1.12 Matrix: blastn matrix:1 \-2 Gap Penalties: Existence: 5, *F Extension: 2 Number of Hits to DB: 1 *F Number of Sequences: 0 Number of extensions: 1 Number of successful *F extensions: 1 Number of sequences better than 10.0: 1 length of query: 1169 *F length of database: 706,884,452 *F effective HSP length: 23 effective length of query: 1146 effective length of *F database: 692,976,559 effective search space: 794151136614 effective search *F space used: 794151136614 T: 0 A: 0 *F X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 12 (23.8 bits) S2: 19 (37.2 bits) *F _______________________________________________________________________ *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|6694276|gb|AAF25198.1|AF193 337 aa *F Sequence 2: gi|6934294|gb|AAF31703.1|AF218 337 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 99 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/23494959027282.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:5480 *F Alignment Score 2024 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/23494959027282.aln *F Your guide tree: *F 23494959027282.dnd *F (gi|6694276|gb|AAF25198.1|AF193:0.00297,gi|6934294|gb|AAF31703.1|AF218:0.00297); *F Your Multiple Sequence Alignment: *F 23494959027282.aln *F CLUSTAL W (1.8) multiple sequence alignment *F gi|6694276|gb|AAF25198.1|AF193 *F MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50 *F gi|6934294|gb|AAF31703.1|AF218 *F MVVDMDTSETAVELTEAENELYDRQIRLWGLESQKRLRTAKILIAGLCGL 50 *F gi|6694276|gb|AAF25198.1|AF193 *F GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100 *F gi|6934294|gb|AAF31703.1|AF218 *F GAEITKNIILSGVNSVKLLDDKDVTEEDFCSQFLVPRESLNTNRAEASLT 100 *F gi|6694276|gb|AAF25198.1|AF193 *F RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150 *F gi|6934294|gb|AAF31703.1|AF218 *F RARALNPMVDISADREPLKEKTSEFFGQFDVVVVNGATNEELLRIDTICR 150 *F gi|6694276|gb|AAF25198.1|AF193 *F DLGVKFIATDVWGTFGFYFASLQKHSYVEDVIKHKVVANSEKKKKYETVS 200 *F gi|6934294|gb|AAF31703.1|AF218 *F DLGVKFIATDVWGTFGFYFASLQKHSYVEDVINHKVVANSEKKKKYETVS 200 *F gi|6694276|gb|AAF25198.1|AF193 *F IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250 *F gi|6934294|gb|AAF31703.1|AF218 *F IPTQRDVDYPGYSAWLDFDVTEPSYLRKLKRNGPGVLLLSVLQKFRTTHK 250 *F gi|6694276|gb|AAF25198.1|AF193 *F RDPSYKTREADLELLRGIRDELLPNSILGNEALGLIFAQISPAVAVVGGV 300 *F gi|6934294|gb|AAF31703.1|AF218 *F RDPSYKTREADLELLRGIRDELLPNSILGDEALGLIFAQISPAVAVVGGV 300 *F gi|6694276|gb|AAF25198.1|AF193 VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337 *F gi|6934294|gb|AAF31703.1|AF218 VAQEVIKVVTKLEAPHRNLFVFDPETCAGYVEAIGAK 337 # *U FBrf0129121 *a Bayraktaroglu *b L. *t 2000.5.23 *T personal communication to FlyBase *u FlyBase error report for CG6815 on Tue May 23 15:18:24 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 23 23:13:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 23 May 2000 23:13:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 23 May 2000 15:18:24 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F Subject: FlyBase error report for CG6815 on Tue May 23 15:18:24 2000 *F Content-Length: 383 *F Error report from Leyla Bayraktaroglu (leyla@morgan.harvard.edu) *F Gene or accession: CG6815 *F Gene annotation error *F Gene CG6815 corresponds to FBgn0040237 *F Comments: CG6815|CT19832 corresponds to bor (FBgn0040237). ClustalW alignment *F is being sent separately to flybase-updates. *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From leyla@morgan.harvard.edu Tue May 23 23:15:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 23 May 2000 23:15:27 \+0100 *F Date: Tue, 23 May 2000 18:13:57 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: ClustalW alignment of bor and CG6815 CDSs *F To: flybase-updates@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-MD5: 0JTdempJ7d/uCR51bVcMwQ== *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F bor FBgn0040237 = CG6815|FBan0006815|CT19832| *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|7230578|gb|AAF43014.1|AF227 604 aa *F Sequence 2: CG6815|FBgn0038409|CT19832|FBa 599 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 95 *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:9512 *F Alignment Score 3437 *F CLUSTAL W (1.8) multiple sequence alignment *F gi|7230578|gb|AAF43014.1|AF227 *F MSWLLGRNRQQPQPDQTAGFSEGGGAADPEGRTAGEKSGDSQLSRAERKA 50 *F CG6815|FBgn0038409|CT19832|FBa *F MSWLLGRNRQQPQPDQTAGFSEGGGAADPEGRTAGEKSGDSQLSRAERKA 50 *F gi|7230578|gb|AAF43014.1|AF227 *F MEAYRFDSSALERAADAAKTLERSKHAREALELSKMQEATRQTEYNTKVK 100 *F CG6815|FBgn0038409|CT19832|FBa *F MEAYRFDSSALERAADAAKTLERSKHAREALELSKMQEATRQTEYNTKVK 100 *F gi|7230578|gb|AAF43014.1|AF227 *F EYEAHIEQAKVEQKRIDHEERRKTLIEETKQQQQRAQYQDQLSRKRYEDQ 150 *F CG6815|FBgn0038409|CT19832|FBa *F EYEAHIEQAKVEQKRIDHEERRKTLIEETKQQQQRAQYQDQLSRKRYEDQ 150 *F gi|7230578|gb|AAF43014.1|AF227 *F LLQQQRVQEENLRKQEESVQRQEAMRRQTIEHEIEMKEKNRLKLLEHELR 200 *F CG6815|FBgn0038409|CT19832|FBa *F LLQQQRVQEENLRKQEESVQRQEAMRRQTIEHEIEMKEKNRLKLLEHELR 200 *F gi|7230578|gb|AAF43014.1|AF227 *F AKARVDRENRDINLEKIRLKAQEHRTTVLEGIKTAGTVIGAGAEAMLTDW 250 *F CG6815|FBgn0038409|CT19832|FBa *F AKARVDRENRDINLEKIRLKAQEHRTTVLEGIKTAGTVIGAGAEAMLTDW 250 *F gi|7230578|gb|AAF43014.1|AF227 *F DKVLTAAGGLSLLALGVYTAKGATGVVSRYVEARIGKPTLVGETSRFAFL 300 *F CG6815|FBgn0038409|CT19832|FBa *F DKVLTAAGGLSLLALGVYTAKGATGVVSRYVEARIGKPTLVGETSRFAFL 300 *F gi|7230578|gb|AAF43014.1|AF227 *F DALKNPLHYLKRLRAKPTDALQGVVLNPKLEERLRDIAIATKNTRINKGM 350 *F CG6815|FBgn0038409|CT19832|FBa *F DALKNPLHYLKRLRAKPTDALQGVVLNPKLEERLRDIAIATKNTRINKGM 350 *F gi|7230578|gb|AAF43014.1|AF227 *F YRNVLMHGPPGTGKTMFAKKLAEHSGMDFAIMTGGDVAPMGKEGVTAIHK 400 *F CG6815|FBgn0038409|CT19832|FBa *F YRNVLMHGPPGTGKTMFAKKLAEHSGMDFAIMTGGDVAPMGKEGVTAIHK 400 *F gi|7230578|gb|AAF43014.1|AF227 *F VFDWSHTSRRGLLLFVDEADAFLRKRSSEKISEDLRAALNAFLYRTSEQN 450 *F CG6815|FBgn0038409|CT19832|FBa *F VFDWSHTSRRGLLLFVDEADAFLRKRSSEKISEDLRAALNAFLYRTSEQN 450 *F gi|7230578|gb|AAF43014.1|AF227 *F PKFMLVLASNTPEQFDYAINDRLDEMVEFTLPGLEERERLLRLYFDKYVL 500 *F CG6815|FBgn0038409|CT19832|FBa *F PKFMLVLASNTPEQFDYAINDRLDEMVEFTLPGLEERERLLRLYFDKYVL 500 *F gi|7230578|gb|AAF43014.1|AF227 *F QPAAAGAKRFKLDTFDYGKTCSKMAALCEGMSGREISKLGVSWQAAVYAS 550 *F CG6815|FBgn0038409|CT19832|FBa *F QPAAAGAKRFKLDTFDYGKTCSKMAALCEGMSGREISKLGVSWQAAVYAS 550 *F gi|7230578|gb|AAF43014.1|AF227 *F EDGLLTEKMVLDRCYSAAQQHKQKMAWLSDQERADHKSITGTAAPPLTLT 600 *F CG6815|FBgn0038409|CT19832|FBa *F EDGLLTEKMVLDRCYSAAQQHKQKRWPGFRIRSVLITNPSQAQLPHPSP- 599 *F \************************ . . .. : : *F \* : *F gi|7230578|gb|AAF43014.1|AF227 AKKL 604 *F CG6815|FBgn0038409|CT19832|FBa \---- *F From leyla@morgan.harvard.edu Tue May 23 23:16:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 23 May 2000 23:16:58 \+0100 *F Date: Tue, 23 May 2000 18:15:40 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: CP89BC and bor merge *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Sounder_of_Swine_836_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 20135 *F Dear Camcur, *F Sequence alignment indicates that CP89BC and bor *F are one and the same. *F First attachment shows the BLASTN alignment of a bor *F accession (AF227209) with the only CP89BC accession (AF229928) *F The second attachment is the ClustalW of their CDSs. *F I have just sent in a FB-updates/error report indicating *F that bor corresponds to CG6815|FBan0006815|CT19832. *F . *F . *F . *F Leyla *F ================================================================================ *F BLASTN alignment of bor (Acc.AF227209) and CP89BC (Acc. AF229928) *F NCBI Entrez BLAST 2 sequences *F BLAST 2 SEQUENCES RESULTS VERSION BLASTN 2.0.11 Jan-20-2000 *F Match: Mismatch: gap open: gap extension: *F x_dropoff: expect: wordsize: Filter *F \------------------------------------------------------------------------ *F Sequence 1gi 7230577 Length 2440 (1 .. 2440) *F Sequence 2gi 6960211 Length 2217 (1 .. 2217) *F 1 *F NOTE:The statistics (bitscore and expect value) is calculated based on the *F size of nr database *F Score = 4146 bits (2156), Expect = 0.0 *F Identities = 2202/2215 (99%), Positives = 2202/2215 (99%), Gaps = 6/2215 (0%) *F Query: 81 *F tacactgagtgaaaagcgcgagaagcgcaagccaacatattaggaaaatgtcgtggcttt 140 *F ||| ||||||||||||||||| ||| | *F |||||||||||||||||||||||||||| ||| *F Sbjct: 9 *F taccctgagtgaaaagcgcgaaaaggg-aagccaacatattaggaaaatgtcgtgg-ttt 66 *F Query: 141 *F tgggcaggaaccgccaacagcctcagccggatcagaccgccggcttttcggaaggaggag 200 *F ||||||||||||||||||||||| |||||||||| *F ||||||||||||||||| ||||||| *F Sbjct: 67 *F tgggcaggaaccgccaacagccttagccggatcaaaccgccggcttttcggacggaggag 126 *F Query: 201 *F gagctgccgatccggagggcaggacggccggcgagaagtccggggattcgcaactgagcc 260 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 127 *F \---ctgccgatccggagggcaggacggccggcgagaagtccggggattcgcaactgagcc 183 *F Query: 261 *F gggcggagcgcaaggccatggaagcgtaccgcttcgattcgtcggcgctggaacgtgcag 320 *F ||||||| *F |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 184 *F gggcgga-cgcaaggccatggaagcgtaccgcttcgattcgtcggcgctggaacgtgcag 242 *F cytoplasmic protein 89BC 1 M E A Y R F D S S A *F L E R A *F Query: 321 *F cggatgctgccaaaaccctggagcgctcaaaacacgcccgggaggccctcgagctgtcca 380 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 243 *F cggatgctgccaaaaccctggagcgctcaaaacacgcccgggaggccctcgagctgtcca 302 *F cytoplasmic protein 89BC 15 A D A A K T L E R S K H A R E A *F L E L S *F Query: 381 *F agatgcaggaggccacccgccaaacggagtacaacaccaaggtcaaggagtacgaagccc 440 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 303 *F agatgcaggaggccacccgccaaacggagtacaacaccaaggtcaaggagtacgaagccc 362 *F cytoplasmic protein 89BC 35 K M Q E A T R Q T E Y N T K V K *F E Y E A *F Query: 441 *F atatcgagcaggccaaggtcgagcagaagcgcatcgaccacgaggagcgccgcaaaactc 500 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 363 *F atatcgagcaggccaaggtcgagcagaagcgcatcgaccacgaggagcgccgcaaaactc 422 *F cytoplasmic protein 89BC 55 H I E Q A K V E Q K R I D H E E *F R R K T *F Query: 501 *F tcatcgaggagaccaagcagcagcagcagcgtgcccaataccaggatcagctgtcccgca 560 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 423 *F tcatcgaggagaccaagcagcagcagcagcgtgcccaataccaggatcagctgtcccgca 482 *F cytoplasmic protein 89BC 75 L I E E T K Q Q Q Q R A Q Y Q D *F Q L S R *F Query: 561 *F agcggtatgaggatcagctgttgcagcagcagcgcgtacaggaggaaaacctgcgcaagc 620 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 483 *F agcggtatgaggatcagctgttgcagcagcagcgcgtacaggaggaaaacctgcgcaagc 542 *F cytoplasmic protein 89BC 95 K R Y E D Q L L Q Q Q R V Q E E *F N L R K *F Query: 621 *F aggaggagagcgtccagcgtcaagaggccatgcggcgccagaccatcgagcacgagatcg 680 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 543 *F aggaggagagcgtccagcgtcaagaggccatgcggcgccagaccatcgagcacgagatcg 602 *F cytoplasmic protein 89BC 115 Q E E S V Q R Q E A M R R Q T I *F E H E I *F Query: 681 *F agatgaaggagaagaaccggcttaagcttctagaacacgaactgcgcgccaaggcacgag 740 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 603 *F agatgaaggagaagaaccggcttaagcttctagaacacgaactgcgcgccaaggcacgag 662 *F cytoplasmic protein 89BC 135 E M K E K N R L K L L E H E L R *F A K A R *F Query: 741 *F tggatcgcgagaaccgggatattaatttggagaagatccgtctcaaggcgcaggagcatc 800 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 663 *F tggatcgcgagaaccgggatattaatttggagaagatccgtctcaaggcgcaggagcatc 722 *F cytoplasmic protein 89BC 155 V D R E N R D I N L E K I R L K *F A Q E H *F Query: 801 *F gaacaaccgtactggagggcatcaaaaccgctggtactgttatcggtgccggtgctgagg 860 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 723 *F gaacaaccgtactggagggcatcaaaaccgctggtactgttatcggtgccggtgctgagg 782 *F cytoplasmic protein 89BC 175 R T T V L E G I K T A G T V I G *F A G A E *F Query: 861 *F ctatgcttaccgactgggacaaggtgctgaccgccgccggaggactttcgttgttggccc 920 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 783 *F ctatgcttaccgactgggacaaggtgctgaccgccgccggaggactttcgttgttggccc 842 *F cytoplasmic protein 89BC 195 A M L T D W D K V L T A A G G L *F S L L A *F Query: 921 *F tgggtgtgtacacggccaagggcgccactggagtggtctctcgatatgtggaggcgcgca 980 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 843 *F tgggtgtgtacacggccaagggcgccactggagtggtctctcgatatgtggaggcgcgca 902 *F cytoplasmic protein 89BC 215 L G V Y T A K G A T G V V S R Y *F V E A R *F Query: 981 *F ttggtaaacctacgttagttggcgagacgtcgcgctttgcttttctggacgcccttaaga 1040 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 903 *F ttggtaaacctacgttagttggcgagacgtcgcgctttgcttttctggacgcccttaaga 962 *F cytoplasmic protein 89BC 235 I G K P T L V G E T S R F A F L *F D A L K *F Query: 1041 *F acccgctgcactacctgaagaggctgcgcgccaagccgaccgatgcgctgcagggcgttg 1100 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 963 *F acccgctgcactacctgaagaggctgcgcgccaagccgaccgatgcgctgcagggcgttg 1022 *F cytoplasmic protein 89BC 255 N P L H Y L K R L R A K P T D A *F L Q G V *F Query: 1101 *F tgctaaatccgaagctggaggaacggcttcgtgacattgccatcgccacaaagaacacac 1160 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1023 *F tgctaaatccgaagctggaggaacggcttcgtgacattgccatcgccacaaagaacacac 1082 *F cytoplasmic protein 89BC 275 V L N P K L E E R L R D I A I A *F T K N T *F Query: 1161 *F gtatcaacaagggcatgtacaggaatgttttgatgcacgggccgccaggaacgggcaaga 1220 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1083 *F gtatcaacaagggcatgtacaggaatgttttgatgcacgggccgccaggaacgggcaaga 1142 *F cytoplasmic protein 89BC 295 R I N K G M Y R N V L M H G P P *F G T G K *F Query: 1221 *F ccatgttcgccaagaagctggccgaacactctggcatggactttgccatcatgaccggtg 1280 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1143 *F ccatgttcgccaagaagctggccgaacactctggcatggactttgccatcatgaccggtg 1202 *F cytoplasmic protein 89BC 315 T M F A K K L A E H S G M D F A *F I M T G *F Query: 1281 *F gcgatgtggcgcccatgggcaaggagggcgtaaccgccatccacaaggtgttcgactggt 1340 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1203 *F gcgatgtggcgcccatgggcaaggagggcgtaaccgccatccacaaggtgttcgactggt 1262 *F cytoplasmic protein 89BC 335 G D V A P M G K E G V T A I H K *F V F D W *F Query: 1341 *F cgcacacctcacgccgcggacttctgctctttgtggacgaggcggatgcattcttacgca 1400 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1263 *F cgcacacctcacgccgcggacttctgctctttgtggacgaggcggatgcattcttacgca 1322 *F cytoplasmic protein 89BC 355 S H T S R R G L L L F V D E A D *F A F L R *F Query: 1401 *F aacgttcttctgagaagatctcggaggatctgcgtgccgccctcaacgccttcctgtacc 1460 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1323 *F aacgttcttctgagaagatctcggaggatctgcgtgccgccctcaacgccttcctgtacc 1382 *F cytoplasmic protein 89BC 375 K R S S E K I S E D L R A A L N *F A F L Y *F Query: 1461 *F gcacttccgagcagaatcccaagttcatgcttgtgttggcctccaacactcccgagcagt 1520 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1383 *F gcacttccgagcagaatcccaagttcatgcttgtgttggcctccaacactcccgagcagt 1442 *F cytoplasmic protein 89BC 395 R T S E Q N P K F M L V L A S N *F T P E Q *F Query: 1521 *F ttgattatgccatcaacgatcgtctggatgaaatggtggagttcacgctgcctggcctgg 1580 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1443 *F ttgattatgccatcaacgatcgtctggatgaaatggtggagttcacgctgcctggcctgg 1502 *F cytoplasmic protein 89BC 415 F D Y A I N D R L D E M V E F T *F L P G L *F Query: 1581 *F aggaacgggaacgcctcttgcgcttgtacttcgacaaatatgtgctgcagcctgccgctg 1640 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1503 *F aggaacgggaacgcctcttgcgcttgtacttcgacaaatatgtgctgcagcctgccgctg 1562 *F cytoplasmic protein 89BC 435 E E R E R L L R L Y F D K Y V L *F Q P A A *F Query: 1641 *F cgggtgccaagcggttcaaactggacacctttgattacggaaagacgtgttcgaagatgg 1700 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1563 *F cgggtgccaagcggttcaaactggacacctttgattacggaaagacgtgttcgaagatgg 1622 *F cytoplasmic protein 89BC 455 A G A K R F K L D T F D Y G K T *F C S K M *F Query: 1701 *F ccgctctgtgcgagggtatgtcgggtcgagaaatctccaagctgggcgtgtcctggcagg 1760 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1623 *F ccgctctgtgcgagggtatgtcgggtcgagaaatctccaagctgggcgtgtcctggcagg 1682 *F cytoplasmic protein 89BC 475 A A L C E G M S G R E I S K L G *F V S W Q *F Query: 1761 *F cggcagtctatgcctccgaggatggtctgctcaccgagaagatggtcctggacaggtgct 1820 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1683 *F cggcagtctatgcctccgaggatggtctgctcaccgagaagatggtcctggacaggtgct 1742 *F cytoplasmic protein 89BC 495 A A V Y A S E D G L L T E K M V *F L D R C *F Query: 1821 *F actccgctgctcagcagcataagcagaagatggcctggctttcggatcaggagcgtgctg 1880 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1743 *F actccgctgctcagcagcataagcagaagatggcctggctttcggatcaggagcgtgctg 1802 *F cytoplasmic protein 89BC 515 Y S A A Q Q H K Q K M A W L S D *F Q E R A *F Query: 1881 *F atcacaaatccatcacaggcacagctgccccacccctcaccctaactgcaaagaaactgt 1940 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1803 *F atcacaaatccatcacaggcacagctgccccacccctcaccctaactgcaaagaaactgt 1862 *F cytoplasmic protein 89BC 535 D H K S I T G T A A P P L T L T *F A K K L ^ *F Query: 1941 *F aaagcgcggaggaaccgatgctgaagtagatgcaacctgcattcaaaacttgtttagttt 2000 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1863 *F aaagcgcggaggaaccgatgctgaagtagatgcaacctgcattcaaaacttgtttagttt 1922 *F cytoplasmic protein 89BC 555 ^^ *F Query: 2001 *F tctaatcacttaactttaagttggtacgcacagggctttatcttactaacgaccaccaac 2060 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1923 *F tctaatcacttaactttaagttggtacgcacagggctttatcttactaacgaccaccaac 1982 *F Query: 2061 *F cagcagccgagacaccaaaatagtgcatatcggttacgtgtatgcatttaacagcctgta 2120 *F |||||||||||||||||||||||||||||||||||| *F ||||||||||||||||||||||| *F Sbjct: 1983 *F cagcagccgagacaccaaaatagtgcatatcggttatgtgtatgcatttaacagcctgta 2042 *F Query: 2121 *F cataatcgttggcgggaagatgtgttcgtctatagatacaattaagtttgacccacagat 2180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2043 *F cataatcgttggcgggaagatgtgttcgtctatagatacaattaagtttgacccacagat 2102 *F Query: 2181 *F aattgtattataagcctacatccactgtctcaatagaatatacaataagaagtatatatt 2240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2103 *F aattgtattataagcctacatccactgtctcaatagaatatacaataagaagtatatatt 2162 *F Query: 2241 *F ttaactgctgtcgttgcatttcgtatgttaaagctttggacgtagactgtaaatt 2295 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2163 *F ttaactgctgtcgttgcatttcgtatgttaaagctttggacgtagactgtaaatt 2217 *F CPU time: 0.14 user secs. 0.03 sys. secs 0.17 total secs. Gapped Lambda K H *F 1.33 0.621 1.12 Matrix: blastn matrix:1 \-2 Gap Penalties: Existence: 5, *F Extension: 2 Number of Hits to DB: 17 Number of Sequences: 0 Number of *F extensions: 17 Number of successful extensions: 5 Number of sequences better *F than 10.0: 1 length of query: 2440 length of database: 706,884,452 effective *F HSP length: 24 effective length of query: 2416 effective length of database: *F 699,931,508 effective search space: 1691034523328 effective search space *F used: 1691034523328 T: 0 A: 0 X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 12 *F (23.8 bits) S2: 20 (39.1 bits) *F ================================================================================ *F Your ClustalW Results: *F Use JalView: *F \------------------------------------------------------------------------ *F Pairwise Scores: *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|7230578|gb|AAF43014.1|AF227 604 aa *F Sequence 2: gi|6960212|gb|AAF33404.1|AF229 554 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 100 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/11496959119374.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:9036 *F Alignment Score 3323 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/11496959119374.aln *F Your guide tree: *F 11496959119374.dnd *F (gi|7230578|gb|AAF43014.1|AF227:0.00000,gi|6960212|gb|AAF33404.1|AF229:0.00000); *F Your Multiple Sequence Alignment: *F 11496959119374.aln *F \------------------------------------------------------------------------ *F CLUSTAL W (1.8) multiple sequence alignment *F gi|7230578|gb|AAF43014.1|AF227 *F MSWLLGRNRQQPQPDQTAGFSEGGGAADPEGRTAGEKSGDSQLSRAERKA 50 *F gi|6960212|gb|AAF33404.1|AF229 *F \-------------------------------------------------- *F gi|7230578|gb|AAF43014.1|AF227 *F MEAYRFDSSALERAADAAKTLERSKHAREALELSKMQEATRQTEYNTKVK 100 *F gi|6960212|gb|AAF33404.1|AF229 *F MEAYRFDSSALERAADAAKTLERSKHAREALELSKMQEATRQTEYNTKVK 50 *F gi|7230578|gb|AAF43014.1|AF227 *F EYEAHIEQAKVEQKRIDHEERRKTLIEETKQQQQRAQYQDQLSRKRYEDQ 150 *F gi|6960212|gb|AAF33404.1|AF229 *F EYEAHIEQAKVEQKRIDHEERRKTLIEETKQQQQRAQYQDQLSRKRYEDQ 100 *F gi|7230578|gb|AAF43014.1|AF227 *F LLQQQRVQEENLRKQEESVQRQEAMRRQTIEHEIEMKEKNRLKLLEHELR 200 *F gi|6960212|gb|AAF33404.1|AF229 *F LLQQQRVQEENLRKQEESVQRQEAMRRQTIEHEIEMKEKNRLKLLEHELR 150 *F gi|7230578|gb|AAF43014.1|AF227 *F AKARVDRENRDINLEKIRLKAQEHRTTVLEGIKTAGTVIGAGAEAMLTDW 250 *F gi|6960212|gb|AAF33404.1|AF229 *F AKARVDRENRDINLEKIRLKAQEHRTTVLEGIKTAGTVIGAGAEAMLTDW 200 *F gi|7230578|gb|AAF43014.1|AF227 *F DKVLTAAGGLSLLALGVYTAKGATGVVSRYVEARIGKPTLVGETSRFAFL 300 *F gi|6960212|gb|AAF33404.1|AF229 *F DKVLTAAGGLSLLALGVYTAKGATGVVSRYVEARIGKPTLVGETSRFAFL 250 *F gi|7230578|gb|AAF43014.1|AF227 *F DALKNPLHYLKRLRAKPTDALQGVVLNPKLEERLRDIAIATKNTRINKGM 350 *F gi|6960212|gb|AAF33404.1|AF229 *F DALKNPLHYLKRLRAKPTDALQGVVLNPKLEERLRDIAIATKNTRINKGM 300 *F gi|7230578|gb|AAF43014.1|AF227 *F YRNVLMHGPPGTGKTMFAKKLAEHSGMDFAIMTGGDVAPMGKEGVTAIHK 400 *F gi|6960212|gb|AAF33404.1|AF229 *F YRNVLMHGPPGTGKTMFAKKLAEHSGMDFAIMTGGDVAPMGKEGVTAIHK 350 *F gi|7230578|gb|AAF43014.1|AF227 *F VFDWSHTSRRGLLLFVDEADAFLRKRSSEKISEDLRAALNAFLYRTSEQN 450 *F gi|6960212|gb|AAF33404.1|AF229 *F VFDWSHTSRRGLLLFVDEADAFLRKRSSEKISEDLRAALNAFLYRTSEQN 400 *F gi|7230578|gb|AAF43014.1|AF227 *F PKFMLVLASNTPEQFDYAINDRLDEMVEFTLPGLEERERLLRLYFDKYVL 500 *F gi|6960212|gb|AAF33404.1|AF229 *F PKFMLVLASNTPEQFDYAINDRLDEMVEFTLPGLEERERLLRLYFDKYVL 450 *F gi|7230578|gb|AAF43014.1|AF227 *F QPAAAGAKRFKLDTFDYGKTCSKMAALCEGMSGREISKLGVSWQAAVYAS 550 *F gi|6960212|gb|AAF33404.1|AF229 *F QPAAAGAKRFKLDTFDYGKTCSKMAALCEGMSGREISKLGVSWQAAVYAS 500 *F gi|7230578|gb|AAF43014.1|AF227 *F EDGLLTEKMVLDRCYSAAQQHKQKMAWLSDQERADHKSITGTAAPPLTLT 600 *F gi|6960212|gb|AAF33404.1|AF229 *F EDGLLTEKMVLDRCYSAAQQHKQKMAWLSDQERADHKSITGTAAPPLTLT 550 *F gi|7230578|gb|AAF43014.1|AF227 AKKL 604 *F gi|6960212|gb|AAF33404.1|AF229 AKKL 554 *F \------------------------------------------------------------------------ # *U FBrf0129122 *a Bayraktaroglu *b L. *t 2000.5.24 *T personal communication to FlyBase *u FlyBase error report on Wed May 24 15:40:26 2000. *F From leyla@morgan.harvard.edu Wed May 24 20:41:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 24 May 2000 20:41:53 \+0100 *F Date: Wed, 24 May 2000 15:40:26 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: BOK and debcl merge *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Descent_of_Woodpeckers_520_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 13429 *F Dear Cambridge curators, *F Please merge BOK and debcl. The BLASTN alignment *F of the BOK cDNA (acc. AF216752) and one of the debcl cDNAs *F (acc. AF149798) is attached. *F (All the debcl cDNAs align with one another.) *F Note that within debcl Accession AF222004 is the *F statement 'Potential Drosophila homologue of Bcl-2-related *F Ovarian Killer (BOK)'. *F Also, the BOK CDS is contained within CG12397 (ie *F CG12397 has to be split and the intron/exon structure *F corrected). BLASTP alignment of the 2 is attached. *F Leyla *F ================================================================================ *F BOKdebclBLN: *F BLAST 2 SEQUENCES RESULTS VERSION BLASTN 2.0.11 Jan-20-2000 *F \------------------------------------------------------------------------ *F Sequence 1 gi 6708481 Length 1618 (1 .. 1618) *F Sequence 2 gi 6724089 Length 1642 (1 .. 1642) *F NOTE:The statistics (bitscore and expect value) is calculated based on the *F size of nr database *F Score = 3009 bits (1565), Expect = 0.0 *F Identities = 1602/1620 (98%), Positives = 1602/1620 (98%), Gaps = 3/1620 (0%) *F Query: 1 *F gagagggtggtaggccgattccctctccccactgcccgttgaaattcagaatactaagct 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 *F gagagggtggtaggccgattccctctccccactgcccgttgaaattcagaatactaagct 60 *F Query: 61 *F ctcggttaaacgcggcgaaaaagaaagcaagctctgagcggctgnnnnnnnnntgaagtg 120 *F |||||||||||||||||||||||||||||||||||||||||||| *F ||||||| *F Sbjct: 61 *F ctcggttaaacgcggcgaaaaagaaagcaagctctgagcggctgaaaaaaaaatgaagtg 120 *F Query: 121 *F aaataaaactgggatcgcggcaccagcaacaagttttagtggctcttctttgtgcgtttc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 *F aaataaaactgggatcgcggcaccagcaacaagttttagtggctcttctttgtgcgtttc 180 *F Query: 181 *F gttcgtgtttgctgccctgcgctttgctcgccacattcgtcgccgacttttattctgttt 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 181 *F gttcgtgtttgctgccctgcgctttgctcgccacattcgtcgccgacttttattttgttt 240 *F Query: 241 *F tgcccattttatcagaatcggagcacctccaaaaaagcccaagacgagctgagcctcagc 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 *F tgcccattttatcagaatcggagcacctccaaaaaagcccaagacgagctgagcctcagc 300 *F Query: 301 *F tgcgtcgaggtgagctgatccactccgctcccctttcgtgcgctgcccaccgctccccac 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301 *F tgcgtcgaggtgagctgatccactccgctcccctttcgtgcgctgcccaccgctccccac 360 *F Query: 361 *F cgctcacacccgatcccatccaatccaatccgatccgctccgctccgagtgcatagtgca 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 361 *F cgctcacacccgatcccatccaatccaatccgatccgctccgctccgagtgcatagtgca 420 *F Query: 421 *F tgcaaagtcgcggggctgggctttcggaattacacaacccacatggggcagcgccaacaa 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 421 *F tgcaaagtcgcggggctgggctttcggaattacacaacccacatggggcagcgc-aacaa 479 *F Bcl-2 family member protein 1 M *F G Q R N K *F Query: 481 *F aggcctcagcagcaacaatagcgggcagccaagcattgccctgccctcgacttcgaccat 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 480 *F aggcctcagcagcaacaatagcgggcagccaagcattgccctgccctcgacttcgaccat 539 *F Bcl-2 family member protein 7 G L S S N N S G Q P S I A L P *F S T S T M *F Query: 541 *F ggctcccaccaccagtccgccacccaagctggccaagttcaagtcctcgtcgctggacca 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 540 *F ggctcccaccaccagtccgccacccaagctggccaagttcaagtcctcgtcgctggacca 599 *F Bcl-2 family member protein 27 A P T T S P P P K L A K F K S *F S S L D H *F Query: 601 *F cgagatctacacggccaatcgccgcggcaccattgccacggcctccagcgactggaaggc 660 *F |||||||||||||||||||||||||||||||||||| *F ||||||||||||||||||||||| *F Sbjct: 600 *F cgagatctacacggccaatcgccgcggcaccattgcaacggcctccagcgactggaaggc 659 *F Bcl-2 family member protein 47 E I Y T A N R R G T I A T A S *F S D W K A *F Query: 661 *F gctccgcggaggcgtcggtggaggagcaggaggacccggtagcgtacccaatccctctaa 720 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 660 *F gctccgcggaggcgtcggtggaggagcaggaggacccggtagcgtacccaatccctctaa 719 *F Bcl-2 family member protein 67 L R G G V G G G A G G P G S V *F P N P S N *F Query: 721 *F cggacgctcccttcacgccggcggacccatgacacgggccgcctccacatcctcgctggc 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 720 *F cggacgctcccttcacgccggcggacccatgacacgggccgcctccacatcctcgctggc 779 *F Bcl-2 family member protein 87 G R S L H A G G P M T R A A S *F T S S L A *F Query: 781 *F tagcagtacgcgcacgatgactaactaccaggagtacaaaatggatatcatcaaccaggg 840 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 780 *F tagcagtacgcgcacgatgactaactaccaggagtacaaaatggatatcatcaaccaggg 839 *F Bcl-2 family member protein 107 S S T R T M T N Y Q E Y K M D *F I I N Q G *F Query: 841 *F gaaatgtctgtgtggtcagtacatcagagcgcggctgcgacgggcaggagtcctcaaccg 900 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 840 *F gaaatgtctgtgtggtcagtacatcagagcgcggctgcgacgggcaggagtcctcaaccg 899 *F Bcl-2 family member protein 127 K C L C G Q Y I R A R L R R A *F G V L N R *F Query: 901 *F gaaggtgacacagcgtttgcgcaacatcctggaccccggctcctcgcacgtggtctatga 960 *F |||||||||||||||||||||||| *F ||||||||||||||||||||||||||||||||||| *F Sbjct: 900 *F gaaggtgacacagcgtttgcgcaatatcctggaccccggctcctcgcacgtggtctatga 959 *F Bcl-2 family member protein 147 K V T Q R L R N I L D P G S S *F H V V Y E *F Query: 961 *F agttttcccggcactgaacagcatgggcgaggaactggagcggatgcacccgcgggtgta 1020 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 960 *F agttttcccggcactgaacagcatgggcgaggaactggagcggatgcacccgcgggtgta 1019 *F Bcl-2 family member protein 167 V F P A L N S M G E E L E R M *F H P R V Y *F Query: 1021 *F cacaaacatatcgcgacagctgtcgagggccccgtttggcgagctggaggacagcgacat 1080 *F |||||| *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1020 *F cacaaatatatcgcgacagctgtcgagggccccgtttggcgagctggaggacagcgacat 1079 *F Bcl-2 family member protein 187 T N I S R Q L S R A P F G E L *F E D S D M *F Query: 1081 *F ggcgcccatgttgctcaacctagttgccaaggatctttttcgctccagcatcacctgggg 1140 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1080 *F ggcgcccatgttgctcaacctagttgccaaggatctttttcgctccagcatcacctgggg 1139 *F Bcl-2 family member protein 207 A P M L L N L V A K D L F R S *F S I T W G *F Query: 1141 *F caagataatctcgatatttgccgtatgcggcggctttgccatagactgcgtgcgccaggg 1200 *F |||||||||||||||||||||||||||||||||||||||| *F ||||||||||||||||||| *F Sbjct: 1140 *F caagataatctcgatatttgccgtatgcggcggctttgccgtagactgcgtgcgccaggg 1199 *F Bcl-2 family member protein 227 K I I S I F A V C G G F A V D *F C V R Q G *F Query: 1201 *F acatttcgactacctacagtgcctgattgacggtctggctgagatcat--aggacgacct 1258 *F |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 1200 *F acatttcgactacctacagtgcctgattgacggtctggctgagatcatagaggacgacct 1259 *F Bcl-2 family member protein 247 H F D Y L Q C L I D G L A E I *F I E D D L *F Query: 1259 *F ggtctactggctgatcgacaacggcggatggttgggcctgtcgcggcacatccgaccccg 1318 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1260 *F ggtctactggctgatcgacaacggcggatggttgggcctgtcgcggcacatccgaccccg 1319 *F Bcl-2 family member protein 267 V Y W L I D N G G W L G L S R *F H I R P R *F Query: 1319 *F ggtcggcgaatttacgttcttgggatggttgacgctgttcgtgactatctctgcaggcgc 1378 *F ||||||||||||||||||||||||||||||||||||||| *F |||||||||||||||||||| *F Sbjct: 1320 *F ggtcggcgaatttacgttcttgggatggttgacgctgtttgtgactatctctgcaggcgc 1379 *F Bcl-2 family member protein 287 V G E F T F L G W L T L F V T *F I S A G A *F Query: 1379 *F atatatggtctcaaacgtgtgtcggcgcattggaggtcaactgtattcgctgctgttcta 1438 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1380 *F atatatggtctcaaacgtgtgtcggcgcattggaggtcaactgtattcgctgctgttcta 1439 *F Bcl-2 family member protein 307 Y M V S N V C R R I G G Q L Y *F S L L F ^^ *F Query: 1439 *F gattcgcttgggatcgcgtcgttaagaaatacaatcgtaccatttagtcaatgagagctt 1498 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1440 *F gattcgcttgggatcgcgtcgttaagaaatacaatcgtaccatttagtcaatgagagctt 1499 *F Bcl-2 family member protein 326 ^ *F Query: 1499 *F caaatcattcctgcttccatgggcaccagtcgtttagtagtatgtaacggaccctgtttt 1558 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1500 *F caaatcattcctgcttccatgggcaccagtcgtttagtagtatgtaacggaccctgtttt 1559 *F Query: 1559 *F acgtataatattgttattccctttctcctctttttgtacatacaaggctattctaggcgc 1618 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1560 *F acgtataatattgttattccctttctcctctttttgtacatacaaggctattctaggcgc 1619 *F CPU time: 0.09 user secs. 0.07 sys. secs 0.16 total secs. Gapped Lambda K H *F 1.33 0.621 1.12 Matrix: blastn matrix:1 \-2 Gap Penalties: Existence: 5, *F Extension: 2 Number of Hits to DB: 5 Number of Sequences: 0 Number of *F extensions: 5 Number of successful extensions: 3 Number of sequences better *F than 10.0: 1 length of query: 1618 length of database: 706,884,452 effective *F HSP length: 24 effective length of query: 1594 effective length of database: *F 696,399,164 effective search space: 1110060267416 effective search space *F used: 1110060267416 T: 0 A: 0 X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 12 *F (23.8 bits) S2: 19 (37.2 bits) *F =============================================================================== *F BOK is contained within CG12397|FBan0012397|CT26692| (1st 3 exons, 3rd exon to *F be extended) (ie CG12397 has to be split) *F CG12397 annotation now reads: *F AE003789 mRNA join(157782..158619,161030..161173,161229..161606, *F 161958..161994,162414..163101,163158..163550, *F 163605..164151,164213..>164266) *F /gene='CG12397' *F By alignment to mRNA Acc. AF216752, annotation for BOK should be *F join(157782..158619,161030..161173,161229..161866) *F BLASTP alignment of BOK CDS with CG12397: *F >CG12397|FBan0012397|CT26692|FBan0012397 last_updated:000321 *F Length = 846 *F Score = 1232 (433.7 bits), Expect = 7.9e-127, P = 7.9e-127 *F Identities = 237/237 (100%), Positives = 237/237 (100%) *F Query: 1 MAPTTSPPPKLAKFKSSSLDHEIYTANRRGTIATASSDWKALRGGVGGGAGGPGSVPNPS 60 *F MAPTTSPPPKLAKFKSSSLDHEIYTANRRGTIATASSDWKALRGGVGGGAGGPGSVPNPS *F Sbjct: 1 MAPTTSPPPKLAKFKSSSLDHEIYTANRRGTIATASSDWKALRGGVGGGAGGPGSVPNPS 60 *F Query: 61 NGRSLHAGGPMTRAASTSSLASSTRTMTNYQEYKMDIINQGKCLCGQYIRARLRRAGVLN 120 *F NGRSLHAGGPMTRAASTSSLASSTRTMTNYQEYKMDIINQGKCLCGQYIRARLRRAGVLN *F Sbjct: 61 NGRSLHAGGPMTRAASTSSLASSTRTMTNYQEYKMDIINQGKCLCGQYIRARLRRAGVLN 120 *F Query: 121 RKVTQRLRNILDPGSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDSD 180 *F RKVTQRLRNILDPGSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDSD *F Sbjct: 121 RKVTQRLRNILDPGSSHVVYEVFPALNSMGEELERMHPRVYTNISRQLSRAPFGELEDSD 180 *F Query: 181 MAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEII 237 *F MAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEII *F Sbjct: 181 MAPMLLNLVAKDLFRSSITWGKIISIFAVCGGFAIDCVRQGHFDYLQCLIDGLAEII 237 *F ================================================================================ # *U FBrf0129123 *a Bayraktaroglu *b L. *t 2000.5.25 *T personal communication to FlyBase *u FlyBase error report on Thu May 25 15:54:54 2000. *F From leyla@morgan.harvard.edu Thu May 25 20:56:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 May 2000 20:56:16 \+0100 *F Date: Thu, 25 May 2000 15:54:54 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: RhoBTB and RhoGTPase merge please *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-MD5: rzzu6XGoWvYiC31bidvvMw== *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Hello again, *F It looks like RhoBTB and RhoGTPase are the same. *F A few mismatches at the beginning, but rest match well. *F Leyla *F BLASTN alignment of RhoBTB and RhoGTPase: *F Query= gi|6979920|gb|AF221547.1|AF221547 *F (3197 letters) *F >gb|AF217287.1|AF217287 Drosophila melanogaster G protein RhoBTB (RhoBTB) mRNA, *F complete cds *F Length = 3253 *F Score = 6223 bits (3139), Expect = 0.0 *F Identities = 3185/3194 (99%), Gaps = 6/3194 (0%) *F Strand = Plus / Plus *F Query: 8 gcgggctgtaactgcaatttattattttttaatttcatttatagtgcacctggtcagcga 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 25 gcgggctgtaactgcaatttattattttttaatttcatttatagtgcacctggtcagcga 84 *F Query: 68 gcactggctcatccgcagccatccgtcggcaaccagctcgcagcgatgttaaacttgtgc 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 85 gcactggctcatccgcagccatccgtcggcaaccagctcgcagcgatgttaaacttgtgc 144 *F Query: 128 cagccgtaaagatttcgcgtgtttgcgaaccacaaactgcgtcgccatcggtgtgtgggt 187 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 145 cagccgtaaagatttcgcgtgtttgcgaaccacaaactgcgtcgccatcggtgtgtgggt 204 *F Query: 188 gcagaaataaatgcaaattgttgtaaaatgccgtgctttggtgtgcgtgttgataagtgc 247 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 205 gcagaaataaatgcaaattgttgtaaaatgccgtgctttggtgtgcgtgttgataagtgc 264 *F Query: 248 aatacgcaaaagtaagaacattgaggcaaatccgtgcaattcgtgcaatccgtgggatca 307 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 265 aatacgcaaaagtaagaacattgaggcaaatccgtgcaattcgtgcaatccgtgggatca 324 *F Query: 308 ggcgttttcagagattccataagctggttctgctatttgtgcatgcccaaaatggacaac 367 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 325 ggcgttttcagagattccataagctggttctgctatttgtgcatgcccaaaatggacaac 384 *F Query: 368 gagcagccgcatcaagagctagtgaagtgcgtcctggtgggggacacggccgtgggcaag 427 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 385 gagcagccgcatcaagagctagtgaagtgcgtcctggtgggggacacggccgtgggcaag 444 *F Query: 428 acgcgactgatctgcgcgagggcctgcaacaaacacgtctcgctatctcagctcctctct 487 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 445 acgcgactgatctgcgcgagggcctgcaacaaacacgtctcgctatctcagctcctctct 504 *F Query: 488 acgcacgtgccgaccgtgtgggccattgaccagtatcgcatatataaggatgtactcgag 547 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 505 acgcacgtgccgaccgtgtgggccattgaccagtatcgcatatataaggatgtactcgag 564 *F Query: 548 cgatcatgggaggtggtcgatggagtgaatgtctcacttcgcctatgggacacatttggt 607 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 565 cgatcatgggaggtggtcgatggagtgaatgtctcacttcgcctatgggacacatttggt 624 *F Query: 608 gaccacgacaaggatcggcgctcc--atacggcagatccgatgtggtgctgctgggcttt 665 *F |||||||||||||||||||||| | |||||||||||||||||||||||||||| ||||| *F Sbjct: 625 gaccacgacaaggatcggcgcttcgcatacggcagatccgatgtggtgctgctgtgcttt 684 *F Query: 666 tccattgccagtccgatttccctgcgcaactgcaagatgatgtggtatcgggaaattcgc 725 *F ||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 685 tccattgccagtccgatttccctgcgcaactgcaagatgatgtggtatccggaaattcgc 744 *F Query: 726 cgcttttgtccggaatgttcccgtcaattctagttggctgcaagaacgacctgcgctaca 785 *F |||||||||||||| ||||||||||| ||||||||||||||||||||||||||||||||| *F Sbjct: 745 cgcttttgtccgga-tgttcccgtca-ttctagttggctgcaagaacgacctgcgctaca 802 *F Query: 786 tgtatcgcgacgagaactatctctcgtatttcggcgagaagggaacctttgttcgagccg 845 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 803 tgtatcgcgacgagaactatctctcgtatttcggcgagaagggaacctttgttcgagccg 862 *F Query: 846 cgttgaagagtgacctggttatgccggatgaggcgcgtgccgtcgccaaagagctaggag 905 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 863 cgttgaagagtgacctggttatgccggatgaggcgcgtgccgtcgccaaagagctaggag 922 *F Query: 906 tggcgtactacgagaccagtgttttcacatactttggggtgaacgaggtgtttgaaaacg 965 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 923 tggcgtactacgagaccagtgttttcacatactttggggtgaacgaggtgtttgaaaacg 982 *F Query: 966 ccatccgatccgcgttgattgcacgccgacagcagcgcttctggatgacaaacctgaaga 1025 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 983 ccatccgatccgcgttgattgcacgccgacagcagcgcttctggatgacaaacctgaaga 1042 *F Query: 1026 aggtgcagaagcccctgctgcaggcgccgttccggccaccgaagccaccaccaccggagg 1085 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1043 aggtgcagaagcccctgctgcaggcgccgttccggccaccgaagccaccaccaccggagg 1102 *F Query: 1086 tcaccgtcatggtcggcaactatcggcaggacatctacaacatgttcctgtcgcaggcct 1145 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1103 tcaccgtcatggtcggcaactatcggcaggacatctacaacatgttcctgtcgcaggcct 1162 *F Query: 1146 acaccgacctcgtcctggtcggggcgggtggcaccaagttcgccgtgcacaggttcatgc 1205 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1163 acaccgacctcgtcctggtcggggcgggtggcaccaagttcgccgtgcacaggttcatgc 1222 *F Query: 1206 tagccgccgcgtccagcatcttccagcgcctgcttagcactgagctgactgatatgggcg 1265 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1223 tagccgccgcgtccagcatcttccagcgcctgcttagcactgagctgactgatatgggcg 1282 *F Query: 1266 ggcggagcagcagcgaatctagcatggtcagctcaacattcggggaggccaccattgcgg 1325 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1283 ggcggagcagcagcgaatctagcatggtcagctcaacattcggggaggccaccattgcgg 1342 *F Query: 1326 actttaacgatgacacggaggccctgatccgctacgaatcacgcacacaacgaatgtggg 1385 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1343 actttaacgatgacacggaggccctgatccgctacgaatcacgcacacaacgaatgtggg 1402 *F Query: 1386 aacacttgaagcgccgctccagctaccaggcgttgcctctcatggagtctaagcggtcca 1445 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1403 aacacttgaagcgccgctccagctaccaggcgttgcctctcatggagtctaagcggtcca 1462 *F Query: 1446 acgatttgtacagggagctgcatcatccggtcctgcagagcattcgcctggtgcacgtgg 1505 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1463 acgatttgtacagggagctgcatcatccggtcctgcagagcattcgcctggtgcacgtgg 1522 *F Query: 1506 aaaaccatcggggtgtaaatggtctgcagacgattgtcacccttagcaaacttatctctc 1565 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1523 aaaaccatcggggtgtaaatggtctgcagacgattgtcacccttagcaaacttatctctc 1582 *F Query: 1566 cgcaagctctgcaccagtgcctgagatttatttataccggtaccattgacaaggattgcg 1625 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1583 cgcaagctctgcaccagtgcctgagatttatttataccggtaccattgacaaggattgcg 1642 *F Query: 1626 ataatatcgaggaaattcgagaagctgccgatctattagaactgccgcaactgactcagc 1685 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1643 ataatatcgaggaaattcgagaagctgccgatctattagaactgccgcaactgactcagc 1702 *F Query: 1686 tactttccaggcctcaaaccgttatggagaactccagcgatgagccaaatccgcacattt 1745 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1703 tactttccaggcctcaaaccgttatggagaactccagcgatgagccaaatccgcacattt 1762 *F Query: 1746 gcctgcgcatcaaagaaagcatggaacggcattgcattggtgacgggtgcttcagtgatg 1805 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1763 gcctgcgcatcaaagaaagcatggaacggcattgcattggtgacgggtgcttcagtgatg 1822 *F Query: 1806 tcacctttgaattggacgatggcttaatgaaggcacaccgcgctgtgttggttggtcgtt 1865 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1823 tcacctttgaattggacgatggcttaatgaaggcacaccgcgctgtgttggttggtcgtt 1882 *F Query: 1866 gtgatgtcatgcgcgcaatgttgttaggcgactttcgcgaggctcattccaatgtgatcg 1925 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1883 gtgatgtcatgcgcgcaatgttgttaggcgactttcgcgaggctcattccaatgtgatcg 1942 *F Query: 1926 tattccctggcgttaccatttacacattccacaagctgctgtgctatttgtacacagacc 1985 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1943 tattccctggcgttaccatttacacattccacaagctgctgtgctatttgtacacagacc 2002 *F Query: 1986 agattccgccaatctcggccgtcaagtgcctcaatctgctggagctggccaatagactct 2045 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2003 agattccgccaatctcggccgtcaagtgcctcaatctgctggagctggccaatagactct 2062 *F Query: 2046 gcctgccgcggctgctcaacctggttgaatgccgcgttattgaagatctcactctgattt 2105 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2063 gcctgccgcggctgctcaacctggttgaatgccgcgttattgaagatctcactctgattt 2122 *F Query: 2106 ctcagaacgaaaccaatgaaacggtggatcactgtctaaagctgctcgagccagtgaagc 2165 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2123 ctcagaacgaaaccaatgaaacggtggatcactgtctaaagctgctcgagccagtgaagc 2182 *F Query: 2166 tgcacaacgcacatcaattggccgagtggtgcatgtcatacctgtgcgtcaattacaacc 2225 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2183 tgcacaacgcacatcaattggccgagtggtgcatgtcatacctgtgcgtcaattacaacc 2242 *F Query: 2226 ttatttgtaagttttcactcaagggcctaaaggcgctacaccaggataatcaggagtact 2285 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2243 ttatttgtaagttttcactcaagggcctaaaggcgctacaccaggataatcaggagtact 2302 *F Query: 2286 tgcgcgagcaccgatggcctccagtctggtatctgaaagactatgattactatcagcgat 2345 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2303 tgcgcgagcaccgatggcctccagtctggtatctgaaagactatgattactatcagcgat 2362 *F Query: 2346 gcctgaacgagctgaacaaggagcttaagctaaagacttcgaggcgggaatcgcctagcg 2405 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2363 gcctgaacgagctgaacaaggagcttaagctaaagacttcgaggcgggaatcgcctagcg 2422 *F Query: 2406 atgacgagggctgtctgtgctttacgggcgtattctcttgctggctgttaggtaaatcga 2465 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2423 atgacgagggctgtctgtgctttacgggcgtattctcttgctggctgttaggtaaatcga 2482 *F Query: 2466 agcgaagcgctgatgtgagtggaaccacggataacagcaacgcagataatcagatcttca 2525 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2483 agcgaagcgctgatgtgagtggaaccacggataacagcaacgcagataatcagatcttca 2542 *F Query: 2526 acagcgccggcaactcgctcaaccacattgatctggaggcggaca-ggacctgaatctct 2584 *F ||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| *F Sbjct: 2543 acagcgccggcaactcgctcaaccacattgatctggaggcggacatggacctgaatctct 2602 *F Query: 2585 gacacccag-cctagggcaaggaagatcactgcgcttcattgtgatcctgccggtcctga 2643 *F ||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2603 gacacccagccctagggcaaggaagatcactgcgcttcattgtgatcctgccggtcctga 2662 *F Query: 2644 tctgttttatctgtacgattttaagtattttgatacttttccaaatccatacataacaaa 2703 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2663 tctgttttatctgtacgattttaagtattttgatacttttccaaatccatacataacaaa 2722 *F Query: 2704 cctcaagggcacgatttagagtggtcggggagataagggttggaaatggagggggcgtgg 2763 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2723 cctcaagggcacgatttagagtggtcggggagataagggttggaaatggagggggcgtgg 2782 *F Query: 2764 cgcgtctgcttttgacattacgcaatatatagagcgcaaatttgccttagtgattaacga 2823 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2783 cgcgtctgcttttgacattacgcaatatatagagcgcaaatttgccttagtgattaacga 2842 *F Query: 2824 ttaataagtccaacgtagctcgacccagaacaagggagcatactgcagagtgcttcggat 2883 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2843 ttaataagtccaacgtagctcgacccagaacaagggagcatactgcagagtgcttcggat 2902 *F Query: 2884 ttcttatatttatacgcctatataaaacgcaaagcatagacgagtatgtatgcacatgca 2943 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2903 ttcttatatttatacgcctatataaaacgcaaagcatagacgagtatgtatgcacatgca 2962 *F Query: 2944 cctcggcgctgcgaagcatttcgctgtattgccttacgatatatcggtgtacactcaact 3003 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2963 cctcggcgctgcgaagcatttcgctgtattgccttacgatatatcggtgtacactcaact 3022 *F Query: 3004 tgttctgcacgatccctcccatggaatgtggacagccgaaggacgcagacatgtgtatat 3063 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3023 tgttctgcacgatccctcccatggaatgtggacagccgaaggacgcagacatgtgtatat 3082 *F Query: 3064 ccatatatttgtacctttacctaagacatgtatgtatgtatatttttgaattcctactta 3123 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3083 ccatatatttgtacctttacctaagacatgtatgtatgtatatttttgaattcctactta 3142 *F Query: 3124 cggaaacatttgtctagccatagctctaagcaattctctacgataaggacgcattttgta 3183 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3143 cggaaacatttgtctagccatagctctaagcaattctctacgataaggacgcattttgta 3202 *F Query: 3184 cagaatgatagaat 3197 *F |||||||||||||| *F Sbjct: 3203 cagaatgatagaat 3216 # *U FBrf0129124 *a Boehm *b S. *t 2000.5.30 *T personal communication to FlyBase *u FlyBase error report for CG12258 on Tue May 30 01:48:06 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 30 09:42:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 May 2000 09:42:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 30 May 2000 01:48:06 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: boehm@mdc-berlin.de *F Subject: FlyBase error report for CG12258 on Tue May 30 01:48:06 2000 *F Content-Length: 603 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG12258 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG12258. *F Comments: CG12258 is a C2H2 zinc finger protein with 5 tandem fingers (pos.540 *F to 677), *F It has been already annotated by InterPro but not in GadFly. *F Any reply to my suggestion is welcome as to my former suggestions. *F Cheers *F Siegfried Boehm *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 95) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129125 *a Boehm *b S. *t 2000.5.30 *T personal communication to FlyBase *u FlyBase error report for CG7312 on Tue May 30 03:27:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 30 11:22:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 May 2000 11:22:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 30 May 2000 03:27:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: boehm@mdc-berlin.de *F Subject: FlyBase error report for CG7312 on Tue May 30 03:27:22 2000 *F Content-Length: 549 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG7312 *F Gene annotation error *F Gene CG7312 has incorrect exon/intron structure. *F Comments: The Gadfly translation for CG7312 has 405aa and 3 C2H2 ZFs,it seems *F to me,that the intron between exons 2 and 3 is wrong,there is a continues *F reading frame, *F My proposed CDS joins 268629..268640,270075..271898, the new ORF has 611aa and *F 6 tandem fingers *F Cheers *F Siegfried Boehm *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 95) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129126 *a Boehm *b S. *t 2000.5.30 *T personal communication to FlyBase *u FlyBase error report for CG9167 on Tue May 30 03:51:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 30 11:46:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 May 2000 11:46:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 30 May 2000 03:51:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: boehm@mdc-berlin.de *F Subject: FlyBase error report for CG9167 on Tue May 30 03:51:41 2000 *F Content-Length: 577 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG9167 *F Gene annotation error *F Gene CG9167 has incorrect exon/intron structure. *F Comments: The CG9167 translation in Gadfly differs significantly in the *F C-terminal part from a sequence in a very recent paper on Kr-h in *F Dev.Biol.2000,221,p53-67, *F The corresponding author of the paper is Dr.Geoff Richards (Strasbourg,France), *F E-mail: richards@igbmc.u-strasbg.fr *F Cheers *F Siegfried Boehm,MDC,Berlin *F Browser: Mozilla/4.0 (compatible; MSIE 5.01; Windows 95) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129127 *a Renault *b A. *t 2000.5.27 *T personal communication to FlyBase *u FlyBase error report for CG17419 on Sat May 27 10:34:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat May 27 18:29:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 27 May 2000 18:29:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 27 May 2000 10:34:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: andrew.renault@zoo.ox.ac.uk *F Subject: FlyBase error report for CG17419 on Sat May 27 10:34:32 2000 *F Content-Length: 1012 *F Error report from andrew renault (andrew.renault@zoo.ox.ac.uk) *F Gene or accession: CG17419 *F cDNA or EST error *F Comments: I believe that the coding seq is much larger than that given. *F We have a cDNA that begins at the same place as GM10035 but ends with the *F following seq. The predicted protein size therefore becomes 859aa rather than *F 347aa. *F TTTTTTTTTTTTTTTTTTTTTTTTTTATTTAACGTGTATAATTAATTATTCCGAAAGGGAAATAATCTATGACAAGGAG *F CCATTTCCACATTGTAGTACATTAAAGCAAACGGTGCATACTCTAACAGGTTTATTTATGCCGAATTTTAAAATGGGCA *F CATCGTTACAGGAACATTTAGAGCACAAGACCCGTCCACAATGCCGGCAATGATGTTTCCTCATAGTAATTGTAAATCT *F GTTTGTACAATGCTGGCAATGGTCAGACTCTGCCCACGGTGATTCCTGTGGCAACTGGTCCAATAAATTGTGTAGTAAT *F TGATCCGTGGCCAGCTTAAAGTTAAAAATATTAATTCCATCTTTATTTTCAGTACCAAGGCACGCTCCTGCCTTGACAA *F GAATTTTACAAAGTGGTGATTGGCCTCTCATAAATGAAAGTAACAGCGGTGTATTACCGTCCATATCAGGAATGTTAAT *F TGGATACTTCGGCATAGACTCAAGAAATAATTCACAAATAAGTCCGGCTGTACTATCTTCCACAACCCGG *F Hope this helps *F Browser: Mozilla/4.0 (compatible; MSIE 4.01; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129130 *a Bayraktaroglu *b L. *t 2000.5.26 *T personal communication to FlyBase *u FlyBase error report on Fri May 26 18:33:20 2000. *F From leyla@morgan.harvard.edu Fri May 26 23:34:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 26 May 2000 23:34:37 \+0100 *F Date: Fri, 26 May 2000 18:33:20 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: please merge bl (bancal) and Hrb57A *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Bevy_of_Otters_618_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 18996 *F Hello, *F Could you please merge bl and Hrb57A? *F I have attached the alignments for bl and Hrb57A. There are some *F differences, but I think they are referring to the same gene. *F A note added in proof in FBrf0126775 (associated with Hrb57A *F accession AJ238947) (Hovemann et al.2000) reads: *F 'Independently, the cloning of Hrb57A was reported recently *F by Charroux et al. (1999).' *F They are referring to FBrf0111834, which is the reference for accession *F AF142631 for bl. *F Leyla *F ================================================================================ *F blHrb57AClW: *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|6687391|emb|CAB64936.1| 496 aa *F Sequence 2: gi|6006748|gb|AAF00596.1|AF142 490 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 96 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/12778959377849.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:7799 *F Alignment Score 2901 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/12778959377849.aln *F CLUSTAL W (1.8) multiple sequence alignment *F gi|6687391|emb|CAB64936.1| *F MKREMNDEGEGPQDQKRNRRNEETVRILIPSSIAGAVIGKGGQHIQKMRT 50 *F gi|6006748|gb|AAF00596.1|AF142 *F MKREMNDEGEGPQDQKRNRRNEETVRILIPSSIAGAVIGKGGQHIQKMRT 50 *F gi|6687391|emb|CAB64936.1| *F QYKATVSVDDSQGPERTIQISADIESTLEIITEMLKYFEERDEDFDVRLL 100 *F gi|6006748|gb|AAF00596.1|AF142 *F QYKATVSVDDSQGPERTIQISADIESTLEIITEMLKYFEERDEDFDVRLL 100 *F gi|6687391|emb|CAB64936.1| *F IHQSLAGCVIGKGGQKIKEIRDRIGCRFLKVFSNVAPQSTDRVVQTVGKQ 150 *F gi|6006748|gb|AAF00596.1|AF142 *F IHQSLAGCVIGKGGQKIKEIRDRIGCRFLKVFSNVAPQSTDRVVQTVGKQ 150 *F gi|6687391|emb|CAB64936.1| *F SQVIEAVREVITLTRDTPIKGAIHNYDPMNFDRVYADEYGGYGTGSGSTR 200 *F gi|6006748|gb|AAF00596.1|AF142 *F SQVIEAVREVITLTRDTPIKGAIHNYDPMNFDRVYADEYGGYGTGSGSTR 200 *F gi|6687391|emb|CAB64936.1| *F PSQRGNNRNGGGAGGVAGGAAGGNGGGFNAGGARGNAGGRGGADRFGGAA 250 *F gi|6006748|gb|AAF00596.1|AF142 *F PSQRGNNRNGGGAGGVAGGAAG-NGGGFNAGGARGNAGGRGGRSAS-AAP 248 *F gi|6687391|emb|CAB64936.1| *F GGAVAGAGMGQRDNPFINPWANGGGGDVDGFGNNPGGAGGFAGNSFGGGA 300 *F gi|6006748|gb|AAF00596.1|AF142 *F GGAVAGAGMGQRDNPFINPWANGGGGDVDGFGNNAGGAGGFAGNSFGGGA 298 *F gi|6687391|emb|CAB64936.1| *F GGPFGGGSFGNNGFGGGPSDFGGNSGNFGQAQGTNSLPSLGSFSQNQGGT 350 *F gi|6006748|gb|AAF00596.1|AF142 *F GGPFGGGSFGNNGFGGGPSDFGGNSGNFGQAQGTNSLPSLGSFSQNQGGT 348 *F gi|6687391|emb|CAB64936.1| *F SSLPSLGSFGQNPGGPGGLGNGLSGGANGGVGTLGGANGAGGFNAVGGAN 400 *F gi|6006748|gb|AAF00596.1|AF142 *F SSLPSLGSFGQNPGGPGGLGNGLSGGANGGVGALGGANGAG-FNAVGGAN 397 *F gi|6687391|emb|CAB64936.1| *F GGPNGSPNAATNVPQGHDPNNSTQVTIPKELAGAIIGKGGGRIRRIRNES 450 *F gi|6006748|gb|AAF00596.1|AF142 *F GGPNGSPNAATNVPQGHDPNNSTQVTIPKELAGAIIGKGGGRIRRIRNES 447 *F gi|6687391|emb|CAB64936.1| *F SAYITIDEPLPNSNDRIITISGTPKQIQMAQYLLQQSVHENGRRNI 496 *F gi|6006748|gb|AAF00596.1|AF142 *F SAYITIDEPLPNSNDRIITISGTPKQIQMAQYLLQQRLVSQSE--- 490 *F \************************************ : .:.. *F ================================================================================ *F Hrb57AblBLN: *F BLAST 2 SEQUENCES RESULTS VERSION BLASTN 2.0.11 Jan-20-2000 *F Sequence 1gi 6006747 Length 2124 (1 .. *F 2124) *F Sequence 2gi 6687390 Drosophila melanogaster mRNA for Length 2040 (1 .. *F Hrb57A ribonucleoprotein 2040) *F 1 *F NOTE:The statistics (bitscore and expect value) is calculated based on the *F size of nr database *F Score = 2936 bits (1527), Expect = 0.0 *F Identities = 1605/1630 (98%), Positives = 1605/1630 (98%), Gaps = 12/1630 (0%) *F Query: 52 *F ctctagtgtgtttgtgctatacatattttgaataatattttaccagccagtgtttagtgg 111 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 *F ctctagtgtgtttgtgctatacatattttgaataatattttaccagccagtgtttagtgg 60 *F Query: 112 *F cggacaaaatcgtgttgaataccaattgaaagttaagtgattaagttcggtggcatcaaa 171 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 *F cggacaaaatcgtgttgaataccaattgaaagttaagtgattaagttcggtggcatcaaa 120 *F Query: 172 *F gtcttcgtagctgaaggaaaacaaaggcatcc-gatagagagactgagaatgaagcgtga 230 *F |||||||||||||||||||||||||||||||| *F ||||||||||||||||||||||||||| *F Sbjct: 121 *F gtcttcgtagctgaaggaaaacaaaggcatcccgatagagagactgagaatgaagcgtga 180 *F heterogeneous nuclear ribonucle> 1 *F M K R E *F Query: 231 *F aatgaacgatgaaggggagggaccacaggaccagaagcgcaatcgccgcaacgaggagac 290 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 *F aatgaacgatgaaggggagggaccacaggaccagaagcgcaatcgccgcaacgaggagac 240 *F heterogeneous nuclear ribonucle> 5 M N D E G E G P Q D Q K R *F N R R N E E T *F Query: 291 *F cgttcgcatcctgattccgagcagtattgctggggctgtcattggcaaaggaggacagca 350 *F ||||||||| *F |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 *F cgttcgcatactgattccgagcagtattgctggggctgtcattggcaaaggaggacagca 300 *F heterogeneous nuclear ribonucle> 25 V R I L I P S S I A G A V *F I G K G G Q H *F Query: 351 *F cattcagaagatgaggactcagtataaagccactgtgtctgtcgacgattcccaaggccc 410 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301 *F cattcagaagatgaggactcagtataaagccactgtgtctgtcgacgattcccaaggccc 360 *F heterogeneous nuclear ribonucle> 45 I Q K M R T Q Y K A T V S *F V D D S Q G P *F Query: 411 *F cgaacgaaccatccaaatatcggcggatatagagtccacattggagattatcaccgaaat 470 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 361 *F cgaacgaaccatccaaatatcggcggatatagagtccacattggagattatcaccgaaat 420 *F heterogeneous nuclear ribonucle> 65 E R T I Q I S A D I E S T *F L E I I T E M *F Query: 471 *F gctgaaatactttgaggagcgcgacgaggactttgatgtgcgtctacttatacaccagag 530 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 421 *F gctgaaatactttgaggagcgcgacgaggactttgatgtgcgtctacttatacaccagag 480 *F heterogeneous nuclear ribonucle> 85 L K Y F E E R D E D F D V *F R L L I H Q S *F Query: 531 *F cttggccggctgtgtcattggcaaaggtggacaaaagatcaaggagatccgcgatcgcat 590 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 481 *F cttggccggctgtgtcattggcaaaggtggacaaaagatcaaggagatccgcgatcgcat 540 *F heterogeneous nuclear ribonucle> 105 L A G C V I G K G G Q K I *F K E I R D R I *F Query: 591 *F cggctgccgctttttgaaggtcttctcgaatgtggcaccacagagcacagatcgagtggt 650 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 541 *F cggctgccgctttttgaaggtcttctcgaatgtggcaccacagagcacagatcgagtggt 600 *F heterogeneous nuclear ribonucle> 125 G C R F L K V F S N V A P *F Q S T D R V V *F Query: 651 *F gcagaccgtgggcaagcagagccaggtcatcgaagcggtgcgtgaggtgatcacacttac 710 *F ||||||||| *F |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 601 *F gcagaccgttggcaagcagagccaggtcatcgaagcggtgcgtgaggtgatcacacttac 660 *F heterogeneous nuclear ribonucle> 145 Q T V G K Q S Q V I E A V *F R E V I T L T *F Query: 711 *F acgggacactcccatcaagggggcgatacataactatgatcctatgaactttgatcgcgt 770 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 661 *F acgggacactcccatcaagggggcgatacataactatgatcctatgaactttgatcgcgt 720 *F heterogeneous nuclear ribonucle> 165 R D T P I K G A I H N Y D *F P M N F D R V *F Query: 771 *F atatgccgatgagtacggtggctatggcactggaagtggcagtacccgtccaagtcagcg 830 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 721 *F atatgccgatgagtacggtggctatggcactggaagtggcagtacccgtccaagtcagcg 780 *F heterogeneous nuclear ribonucle> 185 Y A D E Y G G Y G T G S G *F S T R P S Q R *F Query: 831 *F gggaaacaatcgtaacggaggaggcgctggaggtgttgccggcggcgcagc---tggcaa 887 *F ||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| *F Sbjct: 781 *F gggaaacaatcgtaacggaggaggcgctggaggtgttgccggcggcgcagccggtggcaa 840 *F heterogeneous nuclear ribonucle> 205 G N N R N G G G A G G V A *F G G A A G G N *F Query: 888 *F tggtggaggattcaatgctggcggtgcacgcggcaatgctggcggacgtggcgga--cga 945 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| || *F Sbjct: 841 *F tggtggaggattcaatgctggcggtgcacgcggcaatgctggcggacgtggcggagctga 900 *F heterogeneous nuclear ribonucle> 225 G G G F N A G G A R G N A *F G G R G G A D *F Query: 946 *F tcagcttcggcggcnc--ctggtggagctgtggccggtgctggaatgggacagcgcgaca 1003 *F || |||| |||||| | *F ||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 901 *F tc-gctttggcggcgccgctggtggagctgtggccggtgctggaatggggcagcgcgaca 959 *F heterogeneous nuclear ribonucle> 245 R F G G A A G G A V A G A *F G M G Q R D *F Query: 1004 *F atccgttcatcaatccgtgggccaatggcggtggcggtgatgtagatggttttggcaaca 1063 *F |||||||||| *F ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 960 *F atccgttcattaatccgtgggccaatggcggtggcggtgatgtagatggttttggcaaca 1019 *F heterogeneous nuclear ribonucle> 264 N P F I N P W A N G G G G D *F V D G F G N *F Query: 1064 *F acgctggtggtgctggtggatttgccggtaacagctttggcggtggngccggtggaccat 1123 *F || *F ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||| *F Sbjct: 1020 *F accctggtggtgctggtggatttgccggtaacagctttggcggtggcgccggtggaccat 1079 *F heterogeneous nuclear ribonucle> 284 N P G G A G G F A G N S F G *F G G A G G P *F Query: 1124 *F tcggaggtggtagctttggcaacaacggttttggaggtggacccagcgactttggcggaa 1183 *F ||||||||||||||||||||||||||||||| *F |||||||||||||||||||||||||||| *F Sbjct: 1080 *F tcggaggtggtagctttggcaacaacggtttcggaggtggacccagcgactttggcggaa 1139 *F heterogeneous nuclear ribonucle> 304 F G G G S F G N N G F G G G *F P S D F G G *F Query: 1184 *F attccggaaactttggacaggcccagggcactaacagtctgccaagcctaggtagcttca 1243 *F ||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 1140 *F attccggaaactttggacaggcccagggcactaacagtctgccaagccttggtagcttca 1199 *F heterogeneous nuclear ribonucle> 324 N S G N F G Q A Q G T N S L *F P S L G S F *F Query: 1244 *F gccagaaccagggtggcactagcagcctgcccagcttgggcagctttggtcagaatccag 1303 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1200 *F gccagaaccagggtggcactagcagcctgcccagcttgggcagctttggtcagaatccag 1259 *F heterogeneous nuclear ribonucle> 344 S Q N Q G G T S S L P S L G *F S F G Q N P *F Query: 1304 *F gcggacccggtggattgggtaacggactgagtggcggtgctaatggtggcgttggagccc 1363 *F |||||||||||||||||||||||||||||||||||||||||||||| ||||||||| ||| *F Sbjct: 1260 *F gcggacccggtggattgggtaacggactgagtggcggtgctaatggcggcgttggaaccc 1319 *F heterogeneous nuclear ribonucle> 364 G G P G G L G N G L S G G A *F N G G V G T *F Query: 1364 *F tgggtggtgctaatggagcc---ggctttaatgctgtcggtggagctaacggtgggccga 1420 *F |||||||||||||||||||| *F ||||||||||||||||||||||||||||||||||||| *F Sbjct: 1320 *F tgggtggtgctaatggagccggtggctttaatgctgtcggtggagctaacggtgggccga 1379 *F heterogeneous nuclear ribonucle> 384 L G G A N G A G G F N A V G *F G A N G G P *F Query: 1421 *F atggcagcccaaatgccgcgacaaatgtgcctcagggtcatgatcccaacaacagcacac 1480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1380 *F atggcagcccaaatgccgcgacaaatgtgcctcagggtcatgatcccaacaacagcacac 1439 *F heterogeneous nuclear ribonucle> 404 N G S P N A A T N V P Q G H *F D P N N S T *F Query: 1481 *F aggtcaccattccaaaagagctggctggtgccatcattggtaagggaggtggccgcatcc 1540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1440 *F aggtcaccattccaaaagagctggctggtgccatcattggtaagggaggtggccgcatcc 1499 *F heterogeneous nuclear ribonucle> 424 Q V T I P K E L A G A I I G *F K G G G R I *F Query: 1541 *F gtcgcatccgcaacgagtccagtgcgtacatcaccatcgacgagcccctgccaaactcga 1600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1500 *F gtcgcatccgcaacgagtccagtgcgtacatcaccatcgacgagcccctgccaaactcga 1559 *F heterogeneous nuclear ribonucle> 444 R R I R N E S S A Y I T I D *F E P L P N S *F Query: 1601 *F acgatcgtatcatcaccatctcgggcacgccgaagcaaatacaaatggcccagtatctgc 1660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1560 *F acgatcgtatcatcaccatctcgggcacgccgaagcaaatacaaatggcccagtatctgc 1619 *F heterogeneous nuclear ribonucle> 464 N D R I I T I S G T P K Q I *F Q M A Q Y L *F Query: 1661 tgcaacagag 1670 *F |||||||||| *F Sbjct: 1620 tgcaacagag 1629 *F heterogeneous nuclear ribonucle> 484 L Q Q S *F Score = 769 bits (400), Expect = 0.0 *F Identities = 410/415 (98%), Positives = 410/415 (98%) *F ImageImageImageImageImage *F ImageImageImageImageImage *F Query: 1692 *F agaacgtacacgagaatggcaggcgaaacatttaagtgggcaaggacttgaacagctaca 1751 *F ||| *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1626 *F agagcgtacacgagaatggcaggcgaaacatttaagtgggcaaggacttgaacagctaca 1685 *F heterogeneous nuclear ribonucle> 486 Q S V H E N G R R N I ^^^ *F Query: 1752 *F acagcaatagcaacaccattcgacaaaacaacaaattcaacaactacaataaccatcgag 1811 *F ||||||||||||||||||||||||| *F |||||||||||||||||||||||||||||||||| *F Sbjct: 1686 *F acagcaatagcaacaccattcgacataacaacaaattcaacaactacaataaccatcgag 1745 *F Query: 1812 *F aattgctgcgtttcacgtttaaatttaaatgtactaaaaggaagaactggcgaacgccca 1871 *F || ||||||||||||||||||||||||||||||||||||| *F | ||||||||||||||||| *F Sbjct: 1746 *F aagtgctgcgtttcacgtttaaatttaaatgtactaaaagaaggaactggcgaacgccca 1805 *F Query: 1872 *F gggcaagagagcatcctccaaatacgaaagaaatttatgtatttcttcactaaatttaag 1931 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1806 *F gggcaagagagcatcctccaaatacgaaagaaatttatgtatttcttcactaaatttaag 1865 *F Query: 1932 *F tgaatattatacgccccaaatggataagaaactggaaccgagttgccccaaccatcagcg 1991 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1866 *F tgaatattatacgccccaaatggataagaaactggaaccgagttgccccaaccatcagcg 1925 *F Query: 1992 *F cctgcatccccccattcatcctcgtcgtcaccagtttgcaatttgggggaaagtcaacat 2051 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1926 *F cctgcatccccccattcatcctcgtcgtcaccagtttgcaatttgggggaaagtcaacat 1985 *F Query: 2052 *F taaaactctgtatgataagaagaatgaacaaataaagtaaacataaacttgatat 2106 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1986 *F taaaactctgtatgataagaagaatgaacaaataaagtaaacataaacttgatat 2040 *F CPU time: 0.12 user secs. 0.04 sys. secs 0.16 total secs. Gapped Lambda K H *F 1.33 0.621 1.12 Matrix: blastn matrix:1 \-2 Gap Penalties: Existence: 5, *F Extension: 2 Number of Hits to DB: 17 Number of Sequences: 0 Number of *F extensions: 17 Number of successful extensions: 9 Number of sequences better *F than 10.0: 1 length of query: 2124 length of database: 706,884,452 effective *F HSP length: 24 effective length of query: 2100 effective length of database: *F 698,897,060 effective search space: 1467683826000 effective search space *F used: 1467683826000 T: 0 A: 0 X1: 6 (11.5 bits) X2: 26 (50.0 bits) S1: 12 *F (23.8 bits) S2: 19 (37.2 bits) *F ================================================================================ # *U FBrf0129131 *a Crane-Robinson *b C. *t 2000.5.27 *T personal communication to FlyBase *u FlyBase error report on Sat May 27 19:22:22 2000. *F From cranerobinsonc@hotmail.com Sat May 27 19:17:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 27 May 2000 19:17:38 \+0100 *F X-Originating-IP: 156.40.160.146 *F From: 'Colyn Crane-Robinson' *F To: flybase-updates@morgan.harvard.edu *F Subject: DSox14 *F Date: Sat, 27 May 2000 19:22:22 BST *F Mime-Version: 1.0 *F Content-Type: multipart/mixed; *F boundary='----=_NextPart_000_62484355_4b5e901$62ce8d31' *F Content-Length: 64177 *F I attach an annotated sequence of DSox14 (forget our DSox60B notation). It *F is an MS WORD file which I hope is OK for you. This gene is in FlyBase and *F is *F annotated in GadFly but corrections are needed, as follows. *F The genomic sequence at AE003462 corresponds to ours but the annotation at *F AE003462.1 is incorrect in that it acknowledges only the second (of 2) *F exons. *F The entry at AJ252125.1 (Cremazy et al) is a cDNA that is only partly *F correct. There is a sequencing error at 1621 (deletion of 2 bp) so that *F their subsequent 16 *F amino acids are incorreect and their stop codon is premature: the protein is *F much longer. *F Any replies to: colyn.crane-robinson@port.ac.uk *F Citation: Sparkes, A.C., Mumford, K., Crane-Robinson, C. & Newbury, S.F. *F Biophysics Laboratories, University of Portsmouth, PO1 2DT, UK *F To be published. *F I would be glad if you confirmed receipt of this message and attachment. *F Prof. Colyn Crane-Robinson, *F Biophysics Laboratories, *F St. Michael's Building, *F University of Portsmouth, *F Portsmouth, PO1 2DT, UK. *F Tel: (44)-23-92842055 *F Fax: (44)-23-92842053 *F e-mail: colyn.crane-robinson@port.ac.uk or *F cranerobinsonc@hotmail.com *F (Attached MS WORD file is archived.) # *U FBrf0129132 *a Gisselbrecht *b S. *t 2000.3.30 *T personal communication to FlyBase *u FlyBase error report for CG18455 on Thu Mar 30 18:34:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Mar 31 03:29:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 31 Mar 2000 03:29:52 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 30 Mar 2000 18:34:17 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 389 *F Error report from Stephen Gisselbrecht (steve@trillian.mit.edu) *F Gene or accession: CG18455 *F Gene annotation error *F Gene CG18455 corresponds to AF099184 (FBgn0025360) *F Comments: I stumbled across the identity of this predicted gene with the known *F gene *F 'Optix'; I hope I've recorded this correctly. *F Browser: Mozilla/4.01 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129133 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG10076 on Mon May 15 05:33:47 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 13:28:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 13:28:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 05:33:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10076 on Mon May 15 05:33:47 2000 *F Content-Length: 376 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10076 *F Gene annotation error *F Genes CG10076 and spir should be merged. *F Comments: CG10076 sequence (AE003666; AAF53884) and spir sequence (AJ238876; *F CAB62901) *F align 100% so I feel they should be merged. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129134 *a Whitfield *b E. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG10076 on Mon May 15 05:37:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 13:32:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 13:32:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 05:37:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10076 on Mon May 15 05:37:22 2000 *F Content-Length: 785 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10076 *F Gene annotation error *F Gene CG10076 has incorrect exon/intron structure. *F Comments: CG10076 sequence (AE003666; AAF53884) aligns with spir cDNA sequence *F (AJ238876; CAB62901) but is missing the first 403 amino acids. *F While translating the upstream exons as defined by the mRNA feature *F I found the remaining upstream 403 amino acids and also found an error *F in the mRNA feature. *F Position 108730 does not conform to the consensus intron/exon splice *F site, please amend to 108741, which does conform and allows translation *F of the remaining N terminal sequence to the longest possible initiating *F Met. *F thanks *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129135 *a Palmer *b R. *t 2000.5.27 *T personal communication to FlyBase *u FlyBase error report for CG8250 on Mon May 27 06:51:29 2000. *F From FlyBase-error@whitefly.lbl.gov Mon Mar 27 15:47:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 27 Mar 2000 15:47:09 \+0100 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Mon, 27 Mar 2000 06:51:29 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 390 *F Error report from Ruth Palmer (Ruth.Palmer@ucmp.umu.se) *F Gene or accession: CG8250 *F Missed gene *F Comments: The gene CG8250 at 53C/D is reported with the genebank accession *F number: AF23610 *F [FlyBase curator comment: AF23610 is a typo for AF236106.] *F Palmer,R.H., Scully,A. Jr., Edeen,P., Thomas,J., McKeown,M. Jr. and Hunter,T. *F 'Identification of a novel Drosophila Melanogaster RPTK: dALK' *F Browser: Mozilla/4.51 en (WinNT; I) *F Referred by: query.pl # *U FBrf0129136 *a Micklem *b D. *t 2000.5.27 *T personal communication to FlyBase *u FlyBase error report for CG9435 on Mon Mar 27 21:15:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Mar 28 06:10:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Mar 2000 06:10:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 27 Mar 2000 21:15:02 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 405 *F Error report from David Micklem (dmicklem@cmgm.stanford.edu) *F Gene or accession: CG9435 *F Gene annotation error *F Genes CG9435 and CG9432 should be merged. *F Comments: Genes CG9435 and CG9432 are both part of l(2)01289. *F See Genbank sequences AF216973-AF216977 for correct intron-exon structure *F and two of the many possible splice variants. *F Browser: Mozilla/4.08 (Macintosh; I; PPC, Nav) *F Referred by: query.pl # *U FBrf0129137 *a Salvaterra *b P. *t 2000.5.30 *T personal communication to FlyBase *u FlyBase error report for CG12345 on Tue May 30 15:03:05 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 30 22:57:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 May 2000 22:57:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 30 May 2000 15:03:05 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: psalv@coh.org *F Subject: FlyBase error report for CG12345 on Tue May 30 15:03:05 2000 *F Content-Length: 635 *F Error report from Paul Salvaterra (psalv@coh.org) *F Gene or accession: CG12345 *F Gene annotation error *F Gene CG12345 has a mistake in the supporting evidence or functional assignment. *F Comments: The transcript CT41182 contains the coding sequence for the *F vesicular acetylcholine transporter. *F I believe that this should be noted in the functional assignments. *F Supporting evidence can be found in: *F Kitamoto T, Wang W, Salvaterra PM. (1988) Structure and organization of the *F Drosophila cholinergic locus. J Biol Chem 273:2706-13 *F Browser: Mozilla/4.5 en (WinNT; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129138 *a Goldstein *b L. *t 2000.5.31 *T personal communication to FlyBase *u FlyBase error report for CG8339 on Tue May 30 17:01:09 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 31 00:55:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 May 2000 00:55:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 30 May 2000 17:01:09 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: LGOLDSTEIN@ucsd.edu *F Subject: FlyBase error report for CG8339 on Tue May 30 17:01:09 2000 *F Content-Length: 342 *F Error report from LARRY GOLDSTEIN (LGOLDSTEIN@UCSD.EDU) *F Gene or accession: CG8339 *F Gene annotation error *F Gene CG8339 should be split. *F Comments: CG8339 HAS TWO UNRELATED TRANSCRIPTS. ONE IS A NEW BETA-TUBULIN *F (CT42364). THE OTHER IS THE SFL GENE. *F Browser: Mozilla/4.51 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129139 *a Posakony *b J. *t 2000.3.25 *T personal communication to FlyBase *u FlyBase error report for CG5138 on Fri Mar 24 17:02:17 2000. *F From FlyBase-error@whitefly.lbl.gov Sat Mar 25 00:58:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 25 Mar 2000 00:58:03 \+0000 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Fri, 24 Mar 2000 17:02:17 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 371 *F Error report from jposakony@ucsd.edu *F Gene or accession: CG5138 *F Missed gene *F Comments: This predicted gene encodes a member of the Bearded (Brd) family of *F proteins. We have previously named it 'Ocho' eighth distinct family member *F identified in the genome; Lai et al. (2000) Development 127, 291-306. *F Browser: Mozilla/4.08 (Macintosh; U; PPC, Nav) *F Referred by: query.pl # *U FBrf0129140 *a Lengyel *b J. *t 2000.3.26 *T personal communication to FlyBase *u FlyBase error report for CG6741 on Sun May 26 16:37:08 2000. *F From FlyBase-error@whitefly.lbl.gov Mon Mar 27 01:32:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 27 Mar 2000 01:32:49 \+0100 *F From: FlyBase-error@whitefly.lbl.gov *F Date: Sun, 26 Mar 2000 16:37:08 \-0800 (PST) *F X-Authentication-Warning: whitefly.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 589 *F Error report from Judith Lengyel (jlengyel@ucla.edu) *F Gene or accession: CG6741 *F Missed gene *F Comments: The sequence CG6741, even though I could not find it anywhere *F (GeneScene is not working from my home computer) seems almost certainly from *F its map position and the fact that it has two PDZ domains to be the Drosophila *F arc gene. Our paper on arc (Liu and Lengyel) is in press in Developmental *F Biology. The NCBI accession numbers (multiple splice forms) are AF188473-5. *F Thank you for all your wonderful work. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Referred by: query.pl # *U FBrf0129141 *a Millburn *b G. *t 2000.5.31 *T personal communication to FlyBase *u FlyBase error report for CG13505 on Wed May 31 08:53:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 31 16:48:19 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 May 2000 16:48:19 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 31 May 2000 08:53:02 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG13505 on Wed May 31 08:53:02 2000 *F Content-Length: 591 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG13505 *F Gene annotation error *F Gene CG13505 corresponds to FBgn0000008 (FBgn0000008 (arc)) *F Comments: Hi all, *F in the course of curating a FlyBase error-report that merges a (arc, *F FBgn0000008) with CG6741 (FBgn0034702) I have discovered in addition *F that the N-terminal part of arc corresponds to CG13505. *F I will send the ClustalW alignments of arc and CG13505 in a following *F e-mail to flybase-updates, *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Wed May 31 16:49:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 May 2000 16:49:20 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: ClustalW for arc and CG13505 *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 31 May 2000 16:54:25 \+0100 *F Content-Length: 32933 *F Hi, *F here are the ClustalW alignemnts: *F 1. nucleotide: *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: AF188473 4644 bp *F Sequence 2: CG13505|FBgn0034701|CT32873|FB 1011 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 97 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/23214959787563.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:19072 *F Alignment Score 7797 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/23214959787563.aln *F Your guide tree: *F 23214959787563.dnd *F (AF188473:0.01088,CG13505|FBgn0034701|CT32873|FB:0.01088); *F Your Multiple Sequence Alignment: *F 23214959787563.aln *F CLUSTAL W (1.8) multiple sequence alignment *F AF188473 *F CGCGGCGGTCGCATCGGAGTCGAGAACTCGAAGTGAAGTAACCCATAAAA 50 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CTAACGACAATTCGCATAAACAAACATAATAAAGGAAACGTTAGTAAAAC 100 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AACAGAGTGTAAAAAAGGTTTAGCAACCGCGAAAACGACTTACGAATTTT 150 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CACAACAACTTGATGTTTTGTAACGGAGTTTCAAGAAACTAGTAAAAACT 200 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TTATTGTGAATTCTTCTGCTGCTTCTTCTTTGCAAGTGTGGCAAAAATTT 250 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TGTTGCTCTGCGGATTTATCGAGAACATCAACCACTCACGATTCTGGGCA 300 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCTCACTTCTAAGACTGGGAACCCATTAAGTGAAACACCAACATAGTATG 350 *F CG13505|FBgn0034701|CT32873|FB *F \-----------------------------------------------ATG 3 *F AF188473 *F CGTCTCTTCAAAACGCGCAAATCCACGGATACCTACAGCACACTAGCCGC 400 *F CG13505|FBgn0034701|CT32873|FB *F CGTCTCTTCAAAACGCGCAAATCCACGGATACCTACAGCACACTAGCCGC 53 *F AF188473 *F GCAGCAACAGCAACAGCAGCAGCAGCAACAACAACATCAAGCGGAAGGCA 450 *F CG13505|FBgn0034701|CT32873|FB *F GCAGCAACAGCAACAGCAGCAGCAGCAACAACAACATCAAGCGGAAGGCA 103 *F AF188473 *F GCAACATTTCCCACAGCAGCAACAGCAGCAGCAACAAGAGTCACACACCG 500 *F CG13505|FBgn0034701|CT32873|FB *F GCAACATTTCCCACAGCAGCAACAGCAGCAGCAACAAGAGTCACACACCG 153 *F AF188473 *F GCAACATGCAGCAACAGACTGAACAAGAGCATTGTGAGCAGCACCAGCAT 550 *F CG13505|FBgn0034701|CT32873|FB *F GCAACATGCAGCAACAGACTGAACAAGAGCATTGTGAGCAGCACCAGCAT 203 *F AF188473 *F ATCGTCATCGCTGCCTGATCTGCATGACAAGTCGCCCGTCATGATCCTCA 600 *F CG13505|FBgn0034701|CT32873|FB *F ATCGTCATCGCTGCCTGATCTGCATGACAAGTCGCCCGTCATGATCCTCA 253 *F AF188473 *F GCTGCACCACCCTGGCCAGCAATGGAGCCACCGCCACGGCAGCGGTCACA 650 *F CG13505|FBgn0034701|CT32873|FB *F GCTGCACCACCCTGGCCAGCAATGGAGCCACCGCCACGGCAGCGGTCACA 303 *F AF188473 *F GCAACAGCCACCGGCACAGCAGCAACATCTGGCGGCTCGCTGCAGCAGCA 700 *F CG13505|FBgn0034701|CT32873|FB *F GCAACAGCCACCGGCACAGCAGCAACATCTGGCGGCTCGCTGCAGCAGCA 353 *F AF188473 *F ACAACAGCAGCATCTGCAACACCAGCAGCAGCAGCAGCCGTTACGCACGG 750 *F CG13505|FBgn0034701|CT32873|FB *F ACAACAGCAGCATCTGCAACACCAGCAGCAGCAGCAGCCGTTACGCACGG 403 *F AF188473 *F CCACGCCCACGTGTCTGCTGAGTGGCCGTCAGACGCCATCGGCCATATCG 800 *F CG13505|FBgn0034701|CT32873|FB *F CCACGCCCACGTGTCTGCTGAGTGGCCGTCAGACGCCATCGGCCATATCG 453 *F AF188473 *F GTGATGTCGCTCCAAGAGGCCACCAGTCTGCACCGCCAGCAACAGCAGCC 850 *F CG13505|FBgn0034701|CT32873|FB *F GTGATGTCGCTCCAAGAGGCCACCAGTCTGCACCGCCAGCAACAGCAGCC 503 *F AF188473 *F ACACCAGCCACCCACCATCTACGTGCCGGTGCCTACGAAACTTGGCAACA 900 *F CG13505|FBgn0034701|CT32873|FB *F ACACCAGCCACCCACCATCTACGTGCCGGTGCCTACGAAACTTGGCAACA 553 *F AF188473 *F ATGTCAACACTGGCAACAGCTCGGCCACTCTGCTGCTCAGCTATGGCAGC 950 *F CG13505|FBgn0034701|CT32873|FB *F ATGTCAACACTGGCAACAGCTCGGCCACTCTGCTGCTCAGCTATGGCAGC 603 *F AF188473 *F ACCAGCAGCATCGCCAACCTGCAACAGCAGCAGCAGCAGCATGCCGCCCA 1000 *F CG13505|FBgn0034701|CT32873|FB *F ACCAGCAGCATCGCCAACCTGCAACAGCAGCAGCAGCAGCATGCCGCCCA 653 *F AF188473 *F GTACCAGCAGTATGTTGCACAGCGGCTGCACGCCGCTTCCAGCAGTTGTT 1050 *F CG13505|FBgn0034701|CT32873|FB *F GTACCAGCAGTATGTTGCACAGCGGCTGCACGCCGCTTCCAGCAGTTGTT 703 *F AF188473 *F TGTACGAGAAGGGGTCGAATGCCAGCGGTGGGGCGAGCAGCAACAAAAGC 1100 *F CG13505|FBgn0034701|CT32873|FB *F TGTACGAGAAGGGGTCGAATGCCAGCGGTGGGGCGAGCAGCAACAAAAGC 753 *F AF188473 *F AGTCTATCCCTGACCCCAAATGGTCACTTGCCCGACTACAAGTTGGTGAC 1150 *F CG13505|FBgn0034701|CT32873|FB *F AGTCTATCCCTGACCCCAAATGGTCACTTGCCCGACTACAAGTTGGTGAC 803 *F AF188473 *F AGCGATGCCAGTTGTTGTCCTGGACGATGAACACAAATCCAATTCATTGC 1200 *F CG13505|FBgn0034701|CT32873|FB *F AGCGATGCCAGTTGTTGTCCTGGACGATGAACACAAATCCAATTCATTGC 853 *F AF188473 *F CGGCCACTGAAGCGAGTCGCAACAGCAACAGCAGCAGCAACATGAACGGC 1250 *F CG13505|FBgn0034701|CT32873|FB *F CGGCCACTGAAGCGAGTCGCAACAGCAACAGCAGCAGCAACATGAACGGC 903 *F AF188473 *F AGCAGCAACAGCAACAGCCTTGACGTCAGCAACAGCAACTCGCATTCGGG 1300 *F CG13505|FBgn0034701|CT32873|FB *F AGCAGCAACAGCAACAGCCTTGACGTCAGCAACAGCAACTCGCATTCGGG 953 *F AF188473 *F GAGCTCCACTTCTTTGGCCAGCACCACGAGAAATGTTTTCACCTGGGGCA 1350 *F CG13505|FBgn0034701|CT32873|FB *F GAGCTCCACTTCTTTGGCCAGCACCACGAGAAATG------------GTA 991 *F \*\* *F AF188473 *F AGCGCATGAGTCGCAAACTGGATTTGCTGAAGCGGAGTGACTCGCCCGCC 1400 *F CG13505|FBgn0034701|CT32873|FB *F AGTAC-----------------TTTG------------------------ 1000 *F AF188473 *F GCCGCCCACAAATCGCATTCGGATTTGAGGAGTCTGTTCCACTCGCCAAC 1450 *F CG13505|FBgn0034701|CT32873|FB *F \--------------------------------------------GCCAA- 1005 *F AF188473 *F GCACCACAAGAGTGGATCCGGTGGATCCAGCGGACCCAGTTCGGCGAAGG 1500 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------A------------ 1006 *F \* *F AF188473 *F CATCGGCCTCACCCACTGGCGGCCATCAGAACAGCTCCGGCTCCACGACC 1550 *F CG13505|FBgn0034701|CT32873|FB *F \-----------------------------AATAG---------------- 1011 *F AF188473 *F AGCACCCTCAAGAAGTGCAAGTCGGGGCCCATCGAGACCATCAAGCAGCG 1600 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ACACCAGCAGCAGCAGCAGCAGCAGCAATCGGTTCAGGATGTGGGCACGG 1650 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GACAGAGCCAGAGTGCTCAGTCCACGCCCACGCATCAGTTCCAGGCGGCC 1700 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCCCGCCCACAGAAAGCGCTAAAGAACTTCTTCCATAGGATCGGGTCCAC 1750 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CGGCATGCTGAACCATCGCTCCCACAATCTCCTTAAGGCTTCGGAGGCGG 1800 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CTCAACAGGCCACCCCGGCAGCCACCACATTGTATAGGAGCAGCTCCACT 1850 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AGCCAGCTGTCCAGCAGCTCCTATGTGAAGTGCGACGATCCCACCGAGGG 1900 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ACTGAATCTTCAGAGGGAGCAGCGGGAACAGCGTCTTCCGCGGATCGCCA 1950 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCCTGAAGTCCAGTAGCTGCGATGACATAGCCAAGGTGAGCAGTTGCCTG 2000 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ACGGCCAGCACAAGTAGTGGCAGTGCCGCAGGCAGCTTGGGCTCTCCTCC 2050 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AAGTAGTGCAGCAGCTGGTGGAGGCGGAACTGCAAACAGCGGCCAACACG 2100 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ATCCCTCGCGTCGTGGTGCATTTCCTTACGCCTTCCTGCGATCACGTCTC 2150 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TCCGTTTTGCCAGAGGAGAACCACGGAAATGTACCAGGACACCTGAAGCA 2200 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ACAAATACAGCGGCAACGGGAGCAGCACCAGCAGCATCAGAGGGATCTCC 2250 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TCCAGCAGGAGCAGACATCGTCGCCCCTTCCCCAGCGCCGATCCCCCGAA 2300 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CAGGCGATGCTGAACAATGTGTCACGCAACGACAGCATCACCTCCAAGGA 2350 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CTGGGAACCACTTTACCAAAGATTAAGTAGTTGTCTAAGTTCAAACGAGT 2400 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CCGGCTACGACAGCGATGGGGGTGCGACGGGAGCCCGACTGGGCAATAAT 2450 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CTGAGCATCTCCGGCGGAGATACCGAATCTATTGCCTCGGGCACACTCAA 2500 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCGTAACTCGCTCATCTCCCTCAGCTCCTCGGAGGGCGTTGGAATGGGCA 2550 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TGGGCATGAGTCTGGGACTGGGTGCCCCATCGACGAGGAACAGCAGCATC 2600 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TGCAGTGCTCCCGTGTCGCTGGGTGGCTATAACTACGACTATGAGACGGA 2650 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GACGATACGGCGACGATTCAGGCAGGTTAAGCTGGAGCGCAAGTGCCAAG 2700 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AGGACTACATCGGAATTGTCCTGTCGCCCAAAACGGTGATGACCAATAGC 2750 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AATGAGCAGCAGTACAGGTATCTCATCGTGGAACTGGAACCCTATGGCAT 2800 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GGCCCAAAAGGATGGTCGCCTTCGCCTGGGTGACGAGATCGTCAACGTAA 2850 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ATGGAAAACACCTGCGAGGCATTCAATCCTTTGCAGAGGTTCAGCGCCTG 2900 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TTGAGCAGCTTTGTGGACAACTGTATCGACCTGGTGATTGCTCACGATGA 2950 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GGTGACGACGGTAACTGATTTCTACACCAAAATCCGTATCGATGGGATGA 3000 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCACGCAGCGCCATCGGCTGAGTTATGTGCAACGCACACAGAGCACAGAC 3050 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AGTCTGAGCAGCATGCAGAGTCTGCAGCTGCAGCAGGAGAGGATTCAGGG 3100 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TCACAATACGGAACAGGAGCAGGAGGCCCAGGGCGAGGATCAGTGCGATG 3150 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CGCGTTCAATGGCCAGCGTCAGCACAATGCCCACTCCGATGCCGCTGATG 3200 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CAGCATCGTCGGAGCTCCACGCCCAGGCACTCACTGGACGTCGGTGCGCC 3250 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GGAGCATGAGCTCCTCAGGAGGCGGGCGCGCAGCTCCTCAGGTCAGCGCA 3300 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCTTGGCTCTAACACCGACCCCACTCTTTGCCAGCGGCAGCAGCAGTTGC 3350 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TCCTCCTCCCCTAACCACCGGTTGCTGGATAACGAGAACGACCCTGCTAA 3400 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CGACACCGATTCCTATACGCCAGTGTATGCAAATCGGGCGGCAAGCGTGT 3450 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GCGTGGCCTCCTCCCTGGCGGACGATGAGAAGTGGCAGTTACTGGCCCGA 3500 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AAGCGCTGCTCGGAGGGTTCCGCCCTATCCGCTACACCGAACCCGCAGCA 3550 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ATTTGGCCAGCGCACTCACTACGCCAGAAACTCCATCAATCTGGCCAACT 3600 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CGCATTACCGCTCGCTCCGATTTGCCCACTCGCGGCTGAGTTCGTCTCGC 3650 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CTTAGTCTGTTCATGCAGGCACCGCCTAACAGTCTAACCGTCGGAGAAGG 3700 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AGTCGCTAACACCCCATCCTCTACAGCTACCACAACCACTGATCTCACTA 3750 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ACCAGCAGCAACAGCAGCAAAACCAGCAACAGACACACCAATCACTGTAC 3800 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F ATCAAGCACTCGCCAAAGAGCGTCTCATTGTTCTCGCCTAATCCCTATGT 3850 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TAACGCCTCATCCTCACCAGCTTCGGCATCCACATCAGCGGGTGCCGGCT 3900 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CCTCCCTGGCACCGCCAGCCGCTGCCCTAATGCATCACAGGCCATCGCTT 3950 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CCGGTGGCCAAGCTAACAATACGCGACGAGGAAATGGCGGAGGTCATCCG 4000 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TGCGTCGATGAGCGAGGGTAGTGGACGTTGCACCCCGAAGACTATAACCT 4050 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TCTTTAAGGGACCTGGACTGAAATCGTTGGGCTTCAGCATAGTGGGAGGT 4100 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CGAGATTCGCCAAAGGGCAACATGGGAATTTTTGTAAAGACCGTGTTTCC 4150 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CTCAGGCCAGGCAGCCGATGATGGCACACTGCAAGCGGGCGACGAGATTG 4200 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TAGAGATCAATGGAAACTCTGTGCAGGGCATGAGTCATGCCGAAACCATA 4250 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GGACTCTTTAAGAACGTAAGAGAGGGCACCATTGTGCTAAAAATCTTAAG 4300 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F AAGAAAATTACAGAAAGCTAAATCGATGGGTTGCTAGTGCTAGTTAATGC 4350 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CCGTATTTGACTTATTCCCATGGTAATACATAATTATGGACAACCTGCAT 4400 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CGTTTAACCAAGTTAAATGGTAGCTTCTCTAATTAAGTGAAGCCTTTTTT 4450 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F GTAATCGTACAAAGCCCCTTGCAAGAACTATACTTATACACAGACTCTAT 4500 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TTAGAAACTAGAATCCCAAATAGTATGTAAAGAATTGTATGGAGTTACAA 4550 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F TTTTAAATTATTTACCCATTCTAGACTAGGCTAAGGTTCGAAACGTTGTA 4600 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AF188473 *F CAAGTTCTATTTACATACAACCTTAATATATTTACCTGAAAAGC 4644 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------- *F 2. CDS *F CLUSTAL W (1.8) multiple sequence alignment *F AAF37816.arc *F MRLFKTRKSTDTYSTLAAQQQQQQQQQQQHQAEGSNISHSSNSSSNKSHT 50 *F CG13505|FBgn0034701|CT32873|FB *F MRLFKTRKSTDTYSTLAAQQQQQQQQQQQHQAEGSNISHSSNSSSNKSHT 50 *F AAF37816.arc *F PATCSNRLNKSIVSSTSISSSLPDLHDKSPVMILSCTTLASNGATATAAV 100 *F CG13505|FBgn0034701|CT32873|FB *F PATCSNRLNKSIVSSTSISSSLPDLHDKSPVMILSCTTLASNGATATAAV 100 *F AAF37816.arc *F TATATGTAATSGGSLQQQQQQHLQHQQQQQPLRTATPTCLLSGRQTPSAI 150 *F CG13505|FBgn0034701|CT32873|FB *F TATATGTAATSGGSLQQQQQQHLQHQQQQQPLRTATPTCLLSGRQTPSAI 150 *F AAF37816.arc *F SVMSLQEATSLHRQQQQPHQPPTIYVPVPTKLGNNVNTGNSSATLLLSYG 200 *F CG13505|FBgn0034701|CT32873|FB *F SVMSLQEATSLHRQQQQPHQPPTIYVPVPTKLGNNVNTGNSSATLLLSYG 200 *F AAF37816.arc *F STSSIANLQQQQQQHAAQYQQYVAQRLHAASSSCLYEKGSNASGGASSNK 250 *F CG13505|FBgn0034701|CT32873|FB *F STSSIANLQQQQQQHAAQYQQYVAQRLHAASSSCLYEKGSNASGGASSNK 250 *F AAF37816.arc *F SSLSLTPNGHLPDYKLVTAMPVVVLDDEHKSNSLPATEASRNSNSSSNMN 300 *F CG13505|FBgn0034701|CT32873|FB *F SSLSLTPNGHLPDYKLVTAMPVVVLDDEHKSNSLPATEASRNSNSSSNMN 300 *F AAF37816.arc *F GSSNSNSLDVSNSNSHSGSSTSLASTTRNVFTWGKRMSRKLDLLKRSDSP 350 *F CG13505|FBgn0034701|CT32873|FB *F GSSNSNSLDVSNSNSHSGSSTSLASTTRNGKYFGQK-------------- 336 *F \***************************** :*:: *F AAF37816.arc *F AAAHKSHSDLRSLFHSPTHHKSGSGGSSGPSSAKASASPTGGHQNSSGST 400 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F TSTLKKCKSGPIETIKQRHQQQQQQQQSVQDVGTGQSQSAQSTPTHQFQA 450 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F AARPQKALKNFFHRIGSTGMLNHRSHNLLKASEAAQQATPAATTLYRSSS 500 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F TSQLSSSSYVKCDDPTEGLNLQREQREQRLPRIASLKSSSCDDIAKVSSC 550 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F LTASTSSGSAAGSLGSPPSSAAAGGGGTANSGQHDPSRRGAFPYAFLRSR 600 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F LSVLPEENHGNVPGHLKQQIQRQREQHQQHQRDLLQQEQTSSPLPQRRSP 650 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F EQAMLNNVSRNDSITSKDWEPLYQRLSSCLSSNESGYDSDGGATGARLGN 700 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F NLSISGGDTESIASGTLKRNSLISLSSSEGVGMGMGMSLGLGAPSTRNSS 750 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F ICSAPVSLGGYNYDYETETIRRRFRQVKLERKCQEDYIGIVLSPKTVMTN 800 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F SNEQQYRYLIVELEPYGMAQKDGRLRLGDEIVNVNGKHLRGIQSFAEVQR 850 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F LLSSFVDNCIDLVIAHDEVTTVTDFYTKIRIDGMSTQRHRLSYVQRTQST 900 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F DSLSSMQSLQLQQERIQGHNTEQEQEAQGEDQCDARSMASVSTMPTPMPL 950 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F MQHRRSSTPRHSLDVGAPEHELLRRRARSSSGQRSLALTPTPLFASGSSS 1000 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F CSSSPNHRLLDNENDPANDTDSYTPVYANRAASVCVASSLADDEKWQLLA 1050 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F RKRCSEGSALSATPNPQQFGQRTHYARNSINLANSHYRSLRFAHSRLSSS 1100 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F RLSLFMQAPPNSLTVGEGVANTPSSTATTTTDLTNQQQQQQNQQQTHQSL 1150 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F YIKHSPKSVSLFSPNPYVNASSSPASASTSAGAGSSLAPPAAALMHHRPS 1200 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F LPVAKLTIRDEEMAEVIRASMSEGSGRCTPKTITFFKGPGLKSLGFSIVG 1250 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc *F GRDSPKGNMGIFVKTVFPSGQAADDGTLQAGDEIVEINGNSVQGMSHAET 1300 *F CG13505|FBgn0034701|CT32873|FB *F \-------------------------------------------------- *F AAF37816.arc IGLFKNVREGTIVLKILRRKLQKAKSMGC 1329 *F CG13505|FBgn0034701|CT32873|FB \----------------------------- # *U FBrf0129142 *a Whitfield *b E. *t 2000.5.31 *T personal communication to FlyBase *u FlyBase error report for CG10693 on Wed May 31 08:43:29 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 31 16:38:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 May 2000 16:38:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 31 May 2000 08:43:29 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10693 on Wed May 31 08:43:29 2000 *F Content-Length: 686 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10693 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG10693. *F Comments: The isoform of slo annotated by AE003748; AAF56324.1 is a known 1175 *F amino acid *F isoform. *F Adelman et al, Neuron 9:209-216(1992) isolate a longer isoform of 1236 amino *F acid. The sequence has not been submitted to a nucleotide database but is *F available from TrEMBL entry Q9TWA1. *F Please add a CDS feature for the longer isoform. *F The paper suggests there are at least 41 alternatively spliced forms! *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129143 *a Escher *b S.A. *t 2000.3.30 *T personal communication to FlyBase *u FlyBase error report for CG1086 on Thu Mar 30 05:30:29 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 30 14:26:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Mar 2000 14:26:15 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 30 Mar 2000 05:30:29 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 841 *F Error report from Stefan A Escher (stefan.a.escher@genetik.umu.se) *F Gene or accession: CG1086 *F Gene annotation error *F Gene CG1086; CT41950 has a mistake in the supporting evidence or functional *F assignment. *F Comments: I don't think that there are two transcripts of this gene, at least *F not based on EST GH08948. We have sequenced this from the other end, and it *F contains sequence up to exon 7 and then comes intron sequence and a short *F polyA tail (which is also found in intron 7). So a vital part of the protein *F will be missing. *F Also, the first exon of this EST is placed in AE003469.1 (which is several *F 100kb away, isn't it?), so I think this is a chimeric mRNA. *F I will later comment on the other transporters that we have looked at. *F All the best, *F stef *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 95; DigExt) *F Referred by: query.pl # *U FBrf0129144 *a Chihara *b C.J. *t 2000.3.31 *T personal communication to FlyBase *u FlyBase error report for CG9370 on Fri Mar 31 12:50:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Mar 31 21:45:51 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 31 Mar 2000 21:45:51 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 31 Mar 2000 12:50:11 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 288 *F Error report from CAROL J. CHIHARA (chihara@usfca.edu) *F Gene or accession: CG9370 *F Gene annotation error *F Gene CG9370 corresponds to FBgn0002997 *F Comments: This is an addition rather than an error. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129145 *a Seppo *b A. *t 2000.3.31 *T personal communication to FlyBase *u FlyBase error report for CG6890 on Fri Mar 31 12:05:35 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Mar 31 21:01:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 31 Mar 2000 21:01:15 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 31 Mar 2000 12:05:35 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 503 *F Error report from Antti Seppo (Antti.Seppo@Yale.edu) *F Gene or accession: CG6890 *F Gene annotation error *F Gene CG6890 (Tak1) corresponds to AF204158 (FBgn0029114) *F Comments: CG6890 (annotated as Tak1) appears to not share any homology with *F TGFb activated kinases, but is homologous to toll/18-wheeler family of *F membrane proteins and identical to tollo gene that was previously mapped to *F this cytogenetic location. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F [FlyBase curator comment: the annotation of CG6890 as Tak1 in GadFly was an *F error \- see Martinez Arias, 2000.4.7, personal communication to FlyBase and *F Millburn, 2000.4.12, personal communication to FlyBase.] # *U FBrf0129146 *a Martinez Arias *b A. *t 2000.4.7 *T personal communication to FlyBase *u FlyBase error report on Fri Apr 7 00:28:52 2000. *F From ama11@cus.cam.ac.uk Fri Apr 07 00:24:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 00:24:22 \+0100 *F Date: Fri, 7 Apr 2000 00:28:52 \+0100 (BST) *F From: 'A. Martinez-Arias' *F To: flybase-help@morgan.harvard.edu *F Subject: TAK-1 mapping *F MIME-Version: 1.0 *F I am a bit puzzled as to the mapping of a protein kinase, TAK-1. *F I the general information a genbe encoding this kinase is mapped at 71B5, *F however a personal communication from M. O'Connon puts (in the TAK-1 file) *F places it at 19E1-4. It would be good if this was sorted out and *F corrected. *F In GADFly and at 19E there is a protein kinase with homology to TAK-1. *F I hope that this is helpful *F Alfonso Martinez Arias *F From gelbart@morgan.harvard.edu Fri Apr 07 12:30:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 12:30:15 \+0100 *F Date: Thu, 6 Apr 2000 23:29:17 \-0400 (EDT) *F From: William Gelbart *F To: ama11@cus.cam.ac.uk *F Subject: Re: TAK-1 mapping *F Cc: flybase-help@morgan.harvard.edu *F Content-Length: 2700 *F Dear Alfonso, *F Nice to hear from you. Hope all is well. *F The sequence accession in the Tak-1 file indeed comes from *F 19E. *F There is a gene at 71, CG6890, which has been annotated as Tak1 *F as well. However, the translated product of CG6890 (GenBank *F protein accession AAF06815) has no significant similarity to the *F 19E Tak1 protein in pairwise BLAST. Hence, I don't know if it *F is an annotation glitch from the jamboree or some other problem. *F Here are the proteins from the two genes. *F Tak1 19E *F MATASLDALQAAYVDFSEITLREKVGHGSYGVVCKAVWRDKLVAVKEFFASAEQKDIEKEVKQLSRVKHP *F NIIALHGISSYQQATYLIMEFAEGGSLHNFLHGKVKPAYSLAHAMSWARQCAEGLAYLHAMTPKPLIHRD *F VKPLNLLLTNKGRNLKICDFGTVADKSTMMTNNRGSAAWMAPEVFEGSKYTEKCDIFSWAIVLWEVLSRK *F QPFKGIDNAYTIQWKIYKGERPPLLTTCPKRIEDLMTACWKTVPEDRPSMQYIVGVMHEIVKDYTGADKA *F LEYTFVNQQIVTKESDGTVAAQPDSLSSQEGELSPSSTQLTPTTAANANVNAIAISKTTTSSMTENTSST *F SSDITPTNSGQLDNNPLFYMVTNRWDAIPEEESNESRNDSFNLTSSAEATQRLETIRNGMILMACKPMEQ *F LTLDVEANGFDLSPSESSSSSTNAKSDGRERLTVTDTKPVMMTTDLSNNNGGIHAHSNGLLSHANGWQAR *F DEELQEQEHEQEIVNSLDVDVDPDEDENDGTEQSLAEILDPELQPEPPIPNDAESQLIYRDHRHMAKEYL *F SVDTNLYYAQDFKDKLIVQMDRTEREQKQELLRKMKDKEGLQSLYNNLQQQYASRQLAAGHHPQPHPHPH *F PNQLQHPHSHPPMHFLQDEGCGLLPGSVCGGSESVEEGWVVIPPHHNA *F CG6890 'Tak1' 71B *F MLATTHMLYVLIATCVIPIFGAALSKTVLYQAPDECRWSGGGEHDITLVCHLRTINSELENTNFSVIQPQ *F NTVRLRLECNDALFFQSSLSPDSFRSLVELRDLTIEYCKLGNLTDGSFRGLQELRNLTIRTHNGDWSTMS *F LEMASNSFVEFRQLERLDLSLNNIWLIPDGMVCPLKSLQHLNASYNKIQDISNFYFSASLSSRKARVCGS *F TLQSLDLSANKMVSLPTAMLSALGRLTHLNMAKNSMSFLADRAFEGLLSLRVVDLSANRLTSLPPELFAE *F TKQLQEIYLRNNSINVLAPGIFGELAELLVLDLASNELNSQWINAATFVGLKRLMMLDLSANKISRLEAH *F IFRPLASLQILKLEDNYIDQLPGGIFADLTNLHTLILSRNRISVIEQRTLQGLKNLLVLSLDFNRISRMD *F QRSLVNCSQLQDLHLNDNKLQAVPEALAHVQLLKTLDVGENMISQIENTSITQLESLYGLRMTENSLTHI *F RRGVFDRMSSLQILNLSQNKLKSIEAGSLQRNSQLQAIRLDGNQLKSIAGLFTELPNLVWLNISGNRLEK *F FDYSHIPIGLQWLDVRANRITQLGNYFEIESELSLSTFDASYNLLTEITASSIPNSVEVLYLNDNQISKI *F QPYTFFKKPNLTRVDLVRNRLTTLEPNALRLSPIAEDREIPEFYIGHNAYECDCNLDWLQKVNRESRTQP *F QLMDLDQIHCRLAYARGSSHVSLIEAKSDDFLCKYASHCFALCHCCDFQACDCKMECPDRCSCYHDQSWT *F SNVVDCSRASYEQTLPSHIPMDSTQLYLDGNNFRELQSHAFIGRKRLKVLHLNHSRIEVLHNRTFYGLLE *F LEVLQLQSNQLKALNGNEFQGLDNLQELYLQHNAIATIDTLTFTHLYHLKILRLDHNAITSFAVWNFLPS *F YLNELRLASNPWTCSCEFIDKLRDYINRHEYVVDKLKMKCDVISGNSTQQMVIYPGSGEPASLPVVQCSQ *F TLPLGLDNNFNYAEQAGGENASNATSTKMILNQPPKLDYIPILVAILTAFIFVMICISLVFIFRQEMRVW *F CHSRFGVRLFYNAQKDVDKNEREKLFDAFVSYSSKDELFVNEELAPMLEMGEHRYKLCLHQRDFPVGGYL *F PETIVQAIDSSRRTIMVVSENFIKSEWCRFEFKSAHQSVLRDRRRRLIVIVLGEVPQKELDPDLRLYLKT *F NTYLQWGDKLFWQKLRFALPDVSSSQRSNVAGQSCHVPINHASYHHHHHVHQQAMPLPHSVHHHQQQFML *F PPPPQQPGSFRRQPSLHQQQQQQQQIRGNNNTTQQQQQQQAALLMGGGSVGGPAPQMIPLAGGIQQQSLP *F LPPNQQPTPASRNLHM *F This message is being cc'd to the rest of the flybase-help group *F in case they can help resolve this. *F Best wishes, *F Bill *F From ama11@cus.cam.ac.uk Mon Apr 10 08:01:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 10 Apr 2000 08:01:24 \+0100 *F Date: Mon, 10 Apr 2000 08:05:46 \+0100 (BST) *F From: 'A. Martinez-Arias' *F To: William Gelbart *F cc: flybase-help@morgan.harvard.edu *F Subject: Re: TAK-1 mapping *F MIME-Version: 1.0 *F Dear Bill *F Thank you for your reply. *F Yes, I agree, that is why I was surprised that the location given for tak1 *F is 71B5 rather than 19E2. *F I take this opportunity to tell you and your colleagues over there what a *F great thing FlyBase is and how useful and interesting we find it in my *F lab. The genome, of course, has added a new (or maybe many a) dimension to *F it. *F With best wishes *F Alfonso *F From gm119@gen.cam.ac.uk Wed Apr 12 11:43:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Apr 2000 11:43:52 \+0100 *F To: ama11@cus.cam.ac.uk *F Subject: Re: TAK-1 mapping *F Cc: flybase-help@morgan.harvard.edu *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 12 Apr 2000 11:48:12 \+0100 *F Content-Length: 4245 *F Hi Alfonso, *F . *F . *F As Bill said, the basic problem is that the wrong predicted gene (CG6890) *F has been attached to the 'Tak1' gene (FBgn0026323) which we already had *F in FlyBase (from the literature before the genome sequence came out). *F I'd like to record your e-mail as a personal communication from you to *F FlyBase so that people can see how we knew there was a problem with the *F data for these genes. *F I did a BLAST using the sequence for the Tak1 gene at 19E (AF199466) *F and from the results its clear that the Tak1 gene at 19E corresponds to *F the predicted gene CG1388. *F I will fix all this in the database (although it will probably take a *F few weeks for this to filter through to the public view). *F . *F . *F . *F Gillian # *U FBrf0129147 *a Millburn *b G. *t 2000.4.12 *T personal communication to FlyBase *u FlyBase error report for CG1388 on Wed Apr 12 04:16:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 12 12:11:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Apr 2000 12:11:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 12 Apr 2000 04:16:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 504 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG1388 *F Gene annotation error *F Gene CG1388 corresponds to AF199466 (FBgn0026323) *F Comments: Tak1 (FBgn0026323, AF199466) corresponds to CG1388 (FBgn0031120) and *F NOT to CG6890 (as currently annotated). *F See separate accompanying e-mail from me for alignment of Tak1 and CG1388 (it *F won't let me send the alignment in this box). *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Wed Apr 12 12:12:43 2000 *F Envelope-to: flycam@gen.cam.ac.uk *F Delivery-date: Wed, 12 Apr 2000 12:12:43 \+0100 *F To: flycam@gen.cam.ac.uk, flybase-indiana@morgan.harvard.edu, *F flybase-harvard@morgan.harvard.edu, carolyn@ncbi.nlm.nih.gov, *F flybase-bdgp@morgan.harvard.edu, benos@ebi.ac.uk *F Subject: Re: FlyBase error report Tak1 *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 12 Apr 2000 12:17:17 \+0100 *F Content-Length: 19044 *F Here is the alignment of Tak1 and CG1388 as promised in FlyBase error report. *F Gillian *F Pairwise Scores: *F CLUSTAL W (1.8) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: AF199466 3349 bp *F Sequence 2: CG1388|FBan0001388|CT3172|FBan 2971 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 99 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/8735955537038.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:55933 *F Alignment Score 21964 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/8735955537038.aln *F CLUSTAL W (1.8) multiple sequence alignment *F AF199466 *F \-----------------CGGTCTTGTTAAATTATTAAAAAAAAAACAAGT 33 *F CG1388|FBan0001388|CT3172|FBan *F CACTAATGTATCGATGACGGTCTTGTTAAATTATTAAAAAAAAAACAAGT 50 *F AF199466 *F TTGTACTTGAAAAGTACGGCGTATATCTACAGCGAAGAACGCAAGAACCC 83 *F CG1388|FBan0001388|CT3172|FBan *F TTGTACTTGAAAAGTACGGCGTATATCTACAGCGAAGAACGCAAGAACCC 100 *F AF199466 *F GCCAAGGCAGCGTCCGATGCAGACGCCATCTGTTCAAGCATTGAGCCCGG 133 *F CG1388|FBan0001388|CT3172|FBan *F GCCAAGGCAGCGTCCGATGCAGACGCCATCTGTTCAAGCATTGAGCCCGG 150 *F AF199466 *F AATCCGGAGGAATCCCGGCCAGGATGTGATGTGCGCACTGGGCCCTCTCC 183 *F CG1388|FBan0001388|CT3172|FBan *F AATCCGGAGGAATCCCGGCCAGGATGTGATGTGCGCACTGGGCCCTCTCC 200 *F AF199466 *F GCTGGTCGATATAAACTCGTGCCCCCCTCTTCTAGCCACCTTCTTGCTGT 233 *F CG1388|FBan0001388|CT3172|FBan *F GCTGGTCGATATAAACTCGTGCCCCCCTCTTCTAGCCACCTTCTTGCTGT 250 *F AF199466 *F CAACCGCCTGCTCATTCTACCCCACAGTTCCAGTCCCAGTCCTGCACAGC 283 *F CG1388|FBan0001388|CT3172|FBan *F CAACCGCCTGCTCATTCTACCCCACAGTTCCAGTCCCAGTCCTGCACAGC 300 *F AF199466 *F AATCATC---GCCTGGTGAAGTCTACCGGTAGCACGAGTGGTGGAACCAG 330 *F CG1388|FBan0001388|CT3172|FBan *F AATCATCTTCGCCTGGAGAAGTCTACCGGTAGCACGAGTGGTGGAACCAG 350 *F AF199466 *F GCAGACTACAAAGGATACGGAGATCGCGGTGTCTGGGAAATCGGCTGAGC 380 *F CG1388|FBan0001388|CT3172|FBan *F GCAGACTACAAAGGACACGGAGACCGCGGTGTCTGGGAAATCGGCTGAGC 400 *F AF199466 *F ACGGAAACCGATCCACTAGGGTTAGCATCACGCACCACTTTGTCCAGGAA 430 *F CG1388|FBan0001388|CT3172|FBan *F ACGGAAACCGATCCACTAGGGTTGGCATCACGCACCACTTTGTCCAGGAA 450 *F AF199466 *F CCTGCAGCTGAGGAATCTAGATAACATCTGAAGTCTCCTGGGAACTGCGA 480 *F CG1388|FBan0001388|CT3172|FBan *F CCTGCAGCTGAGGAATCTAGATAACATCTGAAGTCTCCTGGGAACTGCGA 500 *F AF199466 *F AACACACCATATCTTAGCAAAATGACGTGGTAAAAGTTGTCAGTCGCAAT 530 *F CG1388|FBan0001388|CT3172|FBan *F AACACACCATATCTTAGCAAAATGACGTGGTAAAAGTTGTCAGTCGCAAT 550 *F AF199466 *F CTATACAAAGGCAAGAAACTTTATGATGAATCCAGGTCTAGATCTTGCAA 580 *F CG1388|FBan0001388|CT3172|FBan *F CTATACAAAGGCAAGAAACTTTATGATGAATCCAGGTCTAGATCTTGCAA 600 *F AF199466 *F AGATTCCGAAAACTCTGTCCCCAAGAGGGAACCAAAGGCACAGTTAGCCA 630 *F CG1388|FBan0001388|CT3172|FBan *F AGATTCCGAAAACTCTGTCCCCAAGAGGGAACCAAAGGCACAGTTAGCCA 650 *F AF199466 *F GCAATGAAATCGGGAACCCACGGAATCGTAGCCACTAAAAAAGGCGAAAT 680 *F CG1388|FBan0001388|CT3172|FBan *F GCAATGAAATCGGGAACCCACGGAATCGTAGCCACTAAAAA-GGCGAAAT 699 *F AF199466 *F CCCGGTAATTCTACCAAAATAGCGCACCACCTGGACACCATAGAAGAGAG 730 *F CG1388|FBan0001388|CT3172|FBan *F CCCGGTAATTCTACCAAAATAGCGCACCACCTGGACACCATAGAAGAGAG 749 *F AF199466 *F CCTTAAGGCAAGGAATCGGTGGCATACTTGGACAGTTGAAGAGAACTTTA 780 *F CG1388|FBan0001388|CT3172|FBan *F CCTTAAGGCAAGGAATCGGTGGCATACTTGGACAGTTGAAGAGAACTTTA 799 *F AF199466 *F AATCTGGTAATCAGCGGCGCCAAATGCAACTGCAACTGGTAAACTAGTAC 830 *F CG1388|FBan0001388|CT3172|FBan *F AATCTGGTAATCAGCGGCGCCAAATGCAACTGCAACTGGTAAACTAGTAC 849 *F AF199466 *F TCATTTGTAAGCCAAAACACTGACAAACAAAAGCTGCATCTGAAAAGCAA 880 *F CG1388|FBan0001388|CT3172|FBan *F TCATTTGTAAGCCAAAACACTGACAAACAAAAGCTGCATCTGAAAAGCAA 899 *F AF199466 *F GTAGAGAACACCAGGATACTTAGCGTTCCACGGAGATGGCCACAGCATCG 930 *F CG1388|FBan0001388|CT3172|FBan *F GTAGAGAACACCAGGATACTTAGCGTTCCACGGAGATGGCCACAGCATCG 949 *F AF199466 *F CTGGACGCACTGCAGGCAGCCTATGTGGACTTCAGTGAGATAACACTAAG 980 *F CG1388|FBan0001388|CT3172|FBan *F CTGGACGCACTGCAGGCAGCCTATGTGGACTTCAGTGAGATAACACTAAG 999 *F AF199466 *F AGAGAAAGTCGGCCATGGGTCCTACGGTGTGGTCTGCAAGGCCGTTTGGC 1030 *F CG1388|FBan0001388|CT3172|FBan *F AGAGAAAGTCGGCCATGGGTCCTACGGAGTGGTCTGCAAGGCCGTTTGGC 1049 *F AF199466 *F GCGACAAGCTGGTTGCCGTCAAGGAGTTCTTCGCCAGCGCCGAGCAGAAG 1080 *F CG1388|FBan0001388|CT3172|FBan *F GCGACAAGCTGGTTGCCGTCAAGGAGTTCTTCGCCAGCGCCGAGCAGAAG 1099 *F AF199466 *F GACATCGAGAAGGAGGTGAAGCAGTTGTCGCGCGTGAAGCACCCGAACAT 1130 *F CG1388|FBan0001388|CT3172|FBan *F GACATCGAGAAGGAGGTGAAGCAGTTGTCGCGCGTGAAGCACCCGAACAT 1149 *F AF199466 *F CATCGCTCTGCACGGGATATCCTCGTACCAGCAGGCCACCTACCTGATAA 1180 *F CG1388|FBan0001388|CT3172|FBan *F CATCGCTCTGCACGGGATATCCTCGTACCAGCAGGCCACCTACCTGATAA 1199 *F AF199466 *F TGGAGTTCGCCGAAGGTGGATCGCTGCACAACTTCCTTCACGGCAAGGTG 1230 *F CG1388|FBan0001388|CT3172|FBan *F TGGAGTTCGCCGAAGGTGGATCGCTGCACAACTTCCTTCACGGCAAGGTG 1249 *F AF199466 *F AAGCCGGCATATTCTCTGGCCCACGCCATGAGCTGGGCGCGCCAATGTGC 1280 *F CG1388|FBan0001388|CT3172|FBan *F AAGCCGGCATATTCTCTGGCCCACGCCATGAGCTGGGCGCGCCAATGTGC 1299 *F AF199466 *F AGAGGGTCTGGCATATTTGCATGCCATGACGCCAAAACCACTAATACATC 1330 *F CG1388|FBan0001388|CT3172|FBan *F AGAGGGTCTGGCATATTTGCATGCCATGACGCCAAAACCACTAATACATC 1349 *F AF199466 *F GCGACGTGAAGCCGCTGAACCTGCTCTTGACCAACAAGGGACGCAATCTG 1380 *F CG1388|FBan0001388|CT3172|FBan *F GCGACGTGAAGCCGCTGAACCTGCTCTTGACCAACAAGGGACGCAATCTG 1399 *F AF199466 *F AAGATATGCGACTTCGGCACGGTGGCGGACAAGTCGACCATGATGACCAA 1430 *F CG1388|FBan0001388|CT3172|FBan *F AAGATATGCGACTTCGGCACGGTGGCGGACAAGTCGACCATGATGACCAA 1449 *F AF199466 *F CAATCGCGGTAGTGCCGCTTGGATGGCGCCCGAGGTCTTCGAAGGCTCCA 1480 *F CG1388|FBan0001388|CT3172|FBan *F CAATCGCGGCAGTGCCGCTTGGATGGCGCCCGAGGTCTTCGAAGGCTCCA 1499 *F AF199466 *F AGTATACGGAGAAGTGTGACATTTTCAGCTGGGCCATTGTTCTATGGGAG 1530 *F CG1388|FBan0001388|CT3172|FBan *F AGTATACGGAGAAGTGTGACATTTTTAGCTGGGCCATTGTTCTATGGGAG 1549 *F AF199466 *F GTTCTGTCCAGGAAGCAGCCCTTTAAAGGCATCGACAATGCCTACACCAT 1580 *F CG1388|FBan0001388|CT3172|FBan *F GTTCTGTCCAGGAAGCAGCCCTTTAAAGGCATCGACAATGCCTACACCAT 1599 *F AF199466 *F CCAGTGGAAGATCTACAAGGGTGAACGCCCGCCGCTGCTGACCACTTGCC 1630 *F CG1388|FBan0001388|CT3172|FBan *F CCAGTGGAAGATCTACAAGGGTGAACGCCCGCCGCTGCTGACCACTTGCC 1649 *F AF199466 *F CCAAGCGCATCGAGGACCTGATGACCGCCTGCTGGAAAACGGTGCCCGAG 1680 *F CG1388|FBan0001388|CT3172|FBan *F CCAAGCGCATCGAGGACCTGATGACCGCCTGCTGGAAAACGGTGCCCGAG 1699 *F AF199466 *F GATCGCCCGTCGATGCAGTACATAGTGGGCGTTATGCACGAGATCGTCAA 1730 *F CG1388|FBan0001388|CT3172|FBan *F GATCGCCCGTCGATGCAGTACATAGTGGGCGTTATGCACGAGATCGTCAA 1749 *F AF199466 *F GGACTATACGGGGGCGGACAAGGCCCTGGAATACACATTTGTTAATCAAC 1780 *F CG1388|FBan0001388|CT3172|FBan *F GGACTATACGGGGGCGGACAAGGCCCTGGAATACACGTTTGTTAATCAAC 1799 *F AF199466 *F AGATTGTCACCAAAGAGAGCGACGGCACGGTGGCCGCTCAACCGGATAGC 1830 *F CG1388|FBan0001388|CT3172|FBan *F AGATTGTCACCAAAGAGAGCGACGGCACGGTGGCCGCTCAACCGGATAGC 1849 *F AF199466 *F CTCAGTTCGCAGGAGGGGGAACTGAGCCCCTCGTCCACACAGTTAACACC 1880 *F CG1388|FBan0001388|CT3172|FBan *F CTCAGTTCGCAGGAGGGGGAACTGAGCCCCTCGTCCACACAGTTAACACC 1899 *F AF199466 *F GACAACGGCGGCCAACGCCAATGTGAACGCGATAGCAATATCAAAAACAA 1930 *F CG1388|FBan0001388|CT3172|FBan *F GACAACGGCGGCCAACGCCAATGTGAACGCGATAGCAATATCAAAAACAA 1949 *F AF199466 *F CGACTAGCTCAATGACCGAAAATACCTCATCAACATCATCGGACATCACG 1980 *F CG1388|FBan0001388|CT3172|FBan *F CGACTAGCTCAATGACCGAAAATACCTCATCAACATCATCGGACATCACG 1999 *F AF199466 *F CCGACGAACTCGGGCCAACTGGACAATAATCCGCTATTCTATATGGTCAC 2030 *F CG1388|FBan0001388|CT3172|FBan *F CCGACGAACTCGGGCCAACTGGACAATAATCCGCTATTCTATATGGTCAC 2049 *F AF199466 *F CAATCGCTGGGACGCGATTCCCGAGGAGGAGAGCAACGAGAGCCGGAACG 2080 *F CG1388|FBan0001388|CT3172|FBan *F CAATCGCTGGGACGCGATTCCCGAGGAGGAGAGCAACGAGAGCCGGAACG 2099 *F AF199466 *F ATAGCTTCAACCTCACCTCGTCGGCTGAGGCCACTCAGCGCCTCGAAACG 2130 *F CG1388|FBan0001388|CT3172|FBan *F ATAGCTTCAACCTCACCTCGTCGGCTGAGGCCACTCAGCGCCTCGAAACG 2149 *F AF199466 *F ATCCGGAACGGCATGATCCTGATGGCCTGCAAGCCCATGGAGCAGCTCAC 2180 *F CG1388|FBan0001388|CT3172|FBan *F ATCCGGAACGGCATGATCCTGATGGCCTGCAAGCCCATGGAGCAGCTCAC 2199 *F AF199466 *F CCTCGACGTGGAGGCGAATGGCTTTGATCTGAGTCCCAGCGAAAGCAGCA 2230 *F CG1388|FBan0001388|CT3172|FBan *F CCTCGACGTGGAGGCGAATGGCTTTGATCTGAGTCCCAGCGAAAGCAGCA 2249 *F AF199466 *F GCAGCAGCACGAACGCAAAGAGCGATGGCCGCGAACGACTCACGGTGACG 2280 *F CG1388|FBan0001388|CT3172|FBan *F GCAGCAGCACGAACGCAAAGAGCGATGGCCGCGAACGACTCACGGTGACG 2299 *F AF199466 *F GACACCAAGCCGGTGATGATGACCACGGATCTGTCCAACAACAACGGCGG 2330 *F CG1388|FBan0001388|CT3172|FBan *F GACACCAAGCCGGTGATGATGACCACGGATCTGTCCAACAACAACGGCGG 2349 *F AF199466 *F CATCCACGCCCACTCGAACGGACTGCTGAGCCATGCGAATGGTTGGCAAG 2380 *F CG1388|FBan0001388|CT3172|FBan *F CATCCACGCCCACTCGAACGGACTGCTGAGCCATGCGAATGGTTGGCAAG 2399 *F AF199466 *F CAAGAGATGAGGAGCTGCAGGAGCAAGAGCATGAGCAGGAGATTGTCAAC 2430 *F CG1388|FBan0001388|CT3172|FBan *F CAAGAGATGAGGAGCTGCAGGAGCAAGAGCATGAGCAGGAGATTGTCAAC 2449 *F AF199466 *F TCGTTGGACGTCGACGTGGATCCCGACGAGGATGAGAACGACGGCACCGA 2480 *F CG1388|FBan0001388|CT3172|FBan *F TCGTTGGACGTCGACGTGGATCCCGACGAGGATGAGAACGACGGCACCGA 2499 *F AF199466 *F ACAGTCACTGGCCGAGATTCTTGATCCGGAGCTCCAGCCAGAGCCGCCGA 2530 *F CG1388|FBan0001388|CT3172|FBan *F ACAGTCACTGGCCGAGATTCTTGATCCGGAGCTCCAGCCAGAGCCGCCGA 2549 *F AF199466 *F TACCCAACGATGCCGAATCGCAGCTCATCTACCGGGACCACCGACACATG 2580 *F CG1388|FBan0001388|CT3172|FBan *F TACCCAACGATGCCGAATCGCAGCTCATCTACCGGGACCACCGACACATG 2599 *F AF199466 *F GCCAAGGAGTACCTGAGCGTCGACACGAACCTCTACTACGCGCAGGACTT 2630 *F CG1388|FBan0001388|CT3172|FBan *F GCCAAGGAGTACCTGAGCGTCGACACGAACCTCTACTACGCGCAGGACTT 2649 *F AF199466 *F CAAGGACAAGCTCATCGTGCAGATGGACCGAACCGAGCGCGAACAGAAGC 2680 *F CG1388|FBan0001388|CT3172|FBan *F CAAGGACAAGCTCATCGTGCAGATGGACCGAACCGAGCGCGAACAGAAGC 2699 *F AF199466 *F AGGAGCTTCTGCGCAAGATGAAGGACAAGGAGGGTCTTCAGAGTCTTTAC 2730 *F CG1388|FBan0001388|CT3172|FBan *F AGGAGCTTCTGCGCAAGATGAAGGACAAGGAGGGTCTTCAGAGTCTTTAC 2749 *F AF199466 *F AACAATCTGCAGCAGCAGTACGCTTCACGCCAACTTGCGGCCGGCCATCA 2780 *F CG1388|FBan0001388|CT3172|FBan *F AACAATCTGCAGCAGCAGTACGCTTCACGCCAACTTGCGGCCGGCCATCA 2799 *F AF199466 *F TCCGCAACCTCATCCTCATCCGCATCCAAATCAGCTTCAGCATCCACACT 2830 *F CG1388|FBan0001388|CT3172|FBan *F TCCGCAACCTCATCCTCATCCGCATCCAAATCAGCTTCAGCATCCACACT 2849 *F AF199466 *F CACATCCGCCTATGCATTTCCTGCAGGATGAGGGCTGTGGACTGCTGCCC 2880 *F CG1388|FBan0001388|CT3172|FBan *F CACATCCGCCTATGCATTTCCTGCAGGATGAGGGCTGTGGACTGCTGCCC 2899 *F AF199466 *F GGATCGGTGTGCGGAGGCTCTGAGTCCGTGGAAGAAGGCTGGGTGGTCAT 2930 *F CG1388|FBan0001388|CT3172|FBan *F GGATCGGTGTGCGGAGGCTCTGAGTCCGTGGAAGAAGGCTGGGTGGTCAT 2949 *F AF199466 *F CCCACCGCATCACAATGCGTAGTCTCGTGCGCTGCCGATTCGATCGATCG 2980 *F CG1388|FBan0001388|CT3172|FBan *F CCCACCGCATCACAATGCGTAG---------------------------- 2971 *F AF199466 *F ACTCTCTATGTCTATAGGCTAGGACTCTACCTTTAATCCGCAATATGTAA 3030 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 *F TATGTTATCTGTAATCTGTAATCCAATATTCGTAGTCTGTATCTGTACGA 3080 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 *F TTAGCCATAGCACTTTGTTTATTTCTATATCTATGACTATTTCGTTGTAA 3130 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 *F TCGTTTTTTGTTAAGTCTAAGCGTATTCAGTCCGTGTTTCTTTTAATTTA 3180 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 *F GCGAACAATTGTTTCGATCCATTAGCAGTCTCGCAAGCTTCGTTTAGGTT 3230 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 *F TTAATATTATGATTAAGCTTAAAACACACTTAGCATTAGCACATGTATCT 3280 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 *F GTTCGAGGAAGCTGACAAGGAGTAGACCAAAAATATATAAAAACCATCGA 3330 *F CG1388|FBan0001388|CT3172|FBan *F \-------------------------------------------------- *F AF199466 AAGCAACGACAAACAAAAA 3349 *F CG1388|FBan0001388|CT3172|FBan \------------------- # *U FBrf0129148 *a Taghert *b P. *t 2000.4.2 *T personal communication to FlyBase *u FlyBase error report for CG3090 on Sun Apr 2 14:27:36 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Apr 02 22:23:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 2 Apr 2000 22:23:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 2 Apr 2000 14:27:36 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 626 *F Error report from Paul Taghert (taghertp@pcg.wustl.edu) *F Gene or accession: CG3090 *F cDNA or EST error *F Comments: GH14320.5 is listed as a 5' EST for CG3090 (SOX14). I find that its *F sequence is the reverse complement to that encoding SOX14 protein sequence and *F to a short cDNA previously listed as SOX14. Also, the GH14320 sequence is not *F consistent with assignment of CG3090 to the \+ strand of AE003462. That *F assignment appears to be correct. Hence I expect GH14320 is either the *F reverse complement of a 5'EST, or it is a 3' EST. *F Browser: Mozilla/4.61 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129149 *a Mount *b S. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG7102 on Wed Apr 5 19:12:33 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 06 03:08:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 6 Apr 2000 03:08:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 5 Apr 2000 19:12:33 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 805 *F Error report from Steve Mount (sm193@umail.umd.edu) *F Gene or accession: CG7102 *F Gene annotation error *F Gene CG7102 has a mistake in the supporting evidence or functional assignment. *F Comments: The comments in GADfly for CG7102, including the gene function, *F protein motifs and homologs, DO NOT APPLY TO THIS GENE. They apply to *F CG18591, and NOT to CG7102. In other words, CG18591 Is SmE, and CG7102 is *F something else (related to kelch). The GenBank entries AAF52576 . CG18591 gene *F product, and AAF52575 . CG7102 gene product, are correct, as is the *F information in AE003619. *F This confusion results from the fact that the CG18591 coding information is *F unspliced and in an intron of the CG7102 gene. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129150 *a Goldstein *b L. *t 2000.4.1 *T personal communication to FlyBase *u FlyBase error report for CG18637 on Fri Mar 31 15:39:12 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 01 00:34:44 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 1 Apr 2000 00:34:44 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 31 Mar 2000 15:39:12 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 437 *F Error report from LARRY GOLDSTEIN (LGOLDSTEIN@UCSD.EDU) *F Gene or accession: CG18637 *F Missed gene *F Comments: THE RECORD FOR THIS GENE IS CLEARLY INCONSISTENT WITH GENBANK. IF *F YOU SEARCH GENBANK FOR CG18637, YOU GET TWO LONG SEQUENCES AND ONE SHORT. ONE *F OF THE LONG IS SHOT (KAKAPO). THIS IS NOT REFLECTED IN THIS GADFLY RECORD. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129151 *a Richardson *b H. *t 2000.4.1 *T personal communication to FlyBase *u FlyBase error report for CG3267 on Sat Apr 1 02:44:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 01 11:40:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 1 Apr 2000 11:40:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 1 Apr 2000 02:44:46 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 570 *F Error report from Helena Richardson (helena.richardson@adelaide.edu.au) *F Gene or accession: CG3267 *F Missed gene *F Comments: The rescued sequence of l(2)04524 reported by Harvey et al, which *F has sequence *F similarity to propionyl CoA carboxylase, does not agree with the Inverse *F PCR sequence from the BDGP which has sequence similarity to mammalian EB1 a *F protein *F that binds to APC. We have also obtained data on the sequence flanking *F l(2)04524 *F and it agrees with BDGP sequence. *F Browser: Mozilla/4.08 en (Win98; I ;Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 01 11:58:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 1 Apr 2000 11:58:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 1 Apr 2000 03:02:48 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 286 *F Error report from Helena Richardson (helena.richardson@adelaide.edu.au) *F Gene or accession: CG3265 *F Missed gene *F Comments: CG3265 = EB1 similarity *F CG3267 = propionyl-CoA carboxylase similarity *F Browser: Mozilla/4.08 en (Win98; I ;Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129152 *a Mount *b S. *t 2000.4.7 *T personal communication to FlyBase *u FlyBase error report for CG5808 on Thu Apr 6 18:14:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 07 02:09:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 7 Apr 2000 02:09:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 18:14:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 446 *F Error report from Steve Mount (sm193@umail.umd.edu) *F Gene or accession: CG5808 *F cDNA or EST error *F Comments: CG5808 is NOT rbp9. It is /gene='BcDNA:GH01073' on AE003749. *F /protein_id='AAF56342.1'. *F rbp9, on the other hand, is AAF51178, on AE003581. The comments for CG5808 *F seem to go with its sequence, but the name rbp9 is on the page. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129153 *a Martinez Arias *b A. *t 2000.4.12 *T personal communication to FlyBase *u FlyBase error report on Wed Apr 12 18:58:41 2000. *F From ama11@cus.cam.ac.uk Wed Apr 12 18:54:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Apr 2000 18:54:01 \+0100 *F Date: Wed, 12 Apr 2000 18:58:41 \+0100 (BST) *F From: 'A. Martinez-Arias' *F To: Gillian Millburn *F cc: flybase-help@morgan.harvard.edu *F Subject: Re: TAK-1 mapping *F MIME-Version: 1.0 *F Dear Gillian *F . *F . *F . *F There is a gene which appears as JNKK2 (FBgn0040310), which BLAST shows to *F be the same as MKK4 (CG9738; FBan0009738). It seems to me that MKK4 is a *F better name and, whenever possible, it should be corrected (I guess). *F . *F . *F . *F With best wishes *F Alfonso *F From gm119@gen.cam.ac.uk Thu Apr 13 11:51:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Apr 2000 11:51:43 \+0100 *F To: ama11@cus.cam.ac.uk *F Subject: Re: TAK-1 mapping *F Cc: flybase-help@morgan.harvard.edu *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 13 Apr 2000 11:56:04 \+0100 *F Content-Length: 3885 *F Dear Alfonso, *F > *F > There is a gene which appears as JNKK2 (FBgn0040310), which BLAST shows to *F > be the same as MKK4 (CG9738; FBan0009738). It seems to me that MKK4 is a *F > better name and, whenever possible, it should be corrected (I guess). *F thanks for this info. I will record it as a personal *F communication from you to FlyBase. When deciding which gene symbol *F 'wins' (and becomes the valid FlyBase symbol) when merging 2 genes, we *F go for the symbol that was published first (including in GenBank *F records). I checked and Mkk4 will be the valid symbol as it was used *F first in a GenBank record in 1997. *F . *F . *F . *F Gillian # *U FBrf0129154 *a Davis *b T. *t 2000.4.12 *T personal communication to FlyBase *u FlyBase error report for CG7688 on Wed Apr 12 01:47:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 12 09:43:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Apr 2000 09:43:15 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 12 Apr 2000 01:47:50 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 685 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: CG7688 *F Gene annotation error *F Genes cg7688 and cg7689 should be merged. *F Comments: gene cg7688 (clone ae003722) is part of the fruitless *F gene cg7689. See fruitless sequences u72492 and af039231. *F gene cg7688 is an alternate splice product of fru. *F The sequence is spliced from nucleotide 45381 of ae003722 *F the exon for cg7688 starts at position 31849 and ends *F at position 30026 of ae003722. This is a single exon. *F Stop codon at 31326. (See also Ryner et al. Cell 1996) *F all sequence positions are from complementary strand. *F Browser: Mozilla/2.02 (Win16; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129155 *a Davis *b T. *t 2000.4.6 *T personal communication to FlyBase *u FlyBase error report for CG7689 on Thu Apr 06:02:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 06 13:57:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 6 Apr 2000 13:57:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 06:02:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1559 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: Accession number AE003722 genes CG7689 and CG6790 *F Missed gene *F Comments: I work on the fruitless gene and have cloned this gene *F from several species. I have been examining the genome sequence *F in comparison with the known cDNAs for D. melanogaster and *F the known genome structure for D. heteroneura and D. silvestris. *F (Both in Genbank af051662-af51670 and in press) *F The structure given in AE003722 is mostly OK except for the *F 3'terminal exon and the 5' male specific peptide and exons. *F I have given below a detailed annotation based upon the *F published D. melanogaster sequence data. *F Coordinates are from AE003722 *F complimentary strand. *F Accession number AF039231 is male specific fru (Ryner et al cell *F 1996) *F Exons: *F 159917..159742 *F 129972..129681 *F (note start codon for male peptide 129961..129959) *F 50339..50192 *F 50119..49919 *F 49611..48784 *F 47367..47113 *F 45513..45381 *F 31849..31326 *F (note stop codon 31828..31826) *F (note this is gene CG6790 fused to CG7689) *F Accession number U72492 is female fru sequence (Ryner et al *F cell 1996) *F exons: *F 159917..159742 *F 129972..128080 *F 50339..50192 *F (note female start codon 50317..50315) *F rest as for male seq above. *F Accession number D84437 female fru (Ito et al. 1997 *F PNAS) *F exons: *F 50339..50192 *F (note start codon 50317..50315) *F 50119..49919 *F 49611..48784 *F 47367..47113 *F 45513..45381 *F 41715..40694 *F (note stop codon 40696..40694) *F (note that the sequence D84437 hReferer: *F Browser: Mozilla/2.02 (Win16; I) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Tue Jun 06 13:08:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 6 Jun 2000 13:08:54 \+0100 *F To: davist2@cardiff.ac.uk *F Subject: FlyBase error report for fru *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 6 Jun 2000 13:14:22 \+0100 *F Content-Length: 3332 *F Dear Dr. Davis, *F a while back (in April) you wrote to FlyBase with an error report for *F two CG's on the scaffold AE003722. I have included the mail you sent *F at the bottom of this e-mail. I am in the process of getting the *F information in your e-mail into FlyBase as a personal communication, so *F that when the re-annotation is done, it can be used by the molecular *F curators to correct the annotation of that part of the genome. I have *F a couple of questions: *F 1. you talk about the genes 'genes CG7689 and CG6790'. I think that *F 'CG6790' is a typo, and should read 'CG7690' \- please can you confirm *F whether this is the case. *F 2. I think that your message may have been truncated as it ends in the *F middle of a sentence. If that is the case and you have more *F information about these 2 genes, I would be grateful if you could send *F me the extra information and I will incorporate it into the personal *F communication. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F copy of mail you sent: *F >From FlyBase-error@hedgehog.lbl.gov Thu Apr 06 13:57:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 6 Apr 2000 13:57:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Apr 2000 06:02:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 1559 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: Accession number AE003722 genes CG7689 and CG6790 *F Missed gene *F Comments: I work on the fruitless gene and have cloned this gene *F from several species. I have been examining the genome sequence *F in comparison with the known cDNAs for D. melanogaster and *F the known genome structure for D. heteroneura and D. silvestris. *F (Both in Genbank af051662-af51670 and in press) *F The structure given in AE003722 is mostly OK except for the *F 3'terminal exon and the 5' male specific peptide and exons. *F I have given below a detailed annotation based upon the *F published D. melanogaster sequence data. *F Coordinates are from AE003722 *F complimentary strand. *F Accession number AF039231 is male specific fru (Ryner et al cell *F 1996) *F Exons: *F 159917..159742 *F 129972..129681 *F (note start codon for male peptide 129961..129959) *F 50339..50192 *F 50119..49919 *F 49611..48784 *F 47367..47113 *F 45513..45381 *F 31849..31326 *F (note stop codon 31828..31826) *F (note this is gene CG6790 fused to CG7689) *F Accession number U72492 is female fru sequence (Ryner et al *F cell 1996) *F exons: *F 159917..159742 *F 129972..128080 *F 50339..50192 *F (note female start codon 50317..50315) *F rest as for male seq above. *F Accession number D84437 female fru (Ito et al. 1997 *F PNAS) *F exons: *F 50339..50192 *F (note start codon 50317..50315) *F 50119..49919 *F 49611..48784 *F 47367..47113 *F 45513..45381 *F 41715..40694 *F (note stop codon 40696..40694) *F (note that the sequence D84437 hReferer: *F Browser: Mozilla/2.02 (Win16; I) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs *F From wpttd@forest.cf.ac.uk Wed Jun 07 09:55:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Jun 2000 09:55:35 \+0100 *F From: 'Terence DAVIS' *F To: Gillian Millburn (Genetics) *F Date: Wed, 7 Jun 2000 10:00:06 GMT0BST *F MIME-Version: 1.0 *F Content-transfer-encoding: 7BIT *F Subject: Re: FlyBase error report for fru *F Priority: normal *F X-mailer: Pegasus Mail for Windows (v3.12) *F To: davist2@cardiff.ac.uk *F Subject: FlyBase error report for fru *F Copies to: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date sent: Tue, 6 Jun 2000 13:14:22 \+0100 *F > Dear Dr. Davis, *F > *F > a while back (in April) you wrote to FlyBase with an error report for *F > two CG's on the scaffold AE003722. I have included the mail you sent *F > at the bottom of this e-mail. I am in the process of getting the *F > information in your e-mail into FlyBase as a personal communication, so *F > that when the re-annotation is done, it can be used by the molecular *F > curators to correct the annotation of that part of the genome. I have *F > a couple of questions: *F > *F > 1. you talk about the genes 'genes CG7689 and CG6790'. I think that *F > 'CG6790' is a typo, and should read 'CG7690' \- please can you confirm *F > whether this is the case. *F Yes you are correct. The CG7690 is the 5 prime end of the fru gene *F and is spliced to CG7689. *F > 2. I think that your message may have been truncated as it ends in the *F > middle of a sentence. If that is the case and you have more *F > information about these 2 genes, I would be grateful if you could send *F > me the extra information and I will incorporate it into the personal *F > communication. *F I am not sure what I said and I have not the original message. *F I will summarise the info. *F The gene fru is complex and has many splice forms. *F The male and female specific forms mentioned first are from Ryner *F et al. they include CG7690 spliced to CG7689. The male form is *F spliced in the middle of the second exon. The 3 prime terminal from *F Ryner is 31849..31326. *F The female form mentioned in Ito et al. has the same exons *F (including the exons in CG7690 though this is not in the Genbank *F sequence D84437) as Ryner et al. except the last exon is *F 41715..40694 and NOT 31849..31326. The female form of the *F protein is the same except for its 3 prime terminal. Note that this *F corrects the terminal part of CG7689. *F Note that the male proteins differs from the female proteins due to *F the addition of the extra 5 prime amino acids in cds CG7689. *F I hope that this is not confusing. If you can check the references it *F should become clearer. Check also Davis et al (2000) Gene 246, *F p143-149. This is the Drosophila heteroneura fru gene but the *F exons and splice sites are identical though I didn't find the male *F specific exons (CG7690) in heteroneura. *F There are sevaral other splice variants of this gene but I will need to *F talk to my colleagues before I can annotate them. *F If I have confused you please contact me again. It is quite difficult *F to be clear over the e.mail. *F Sincerely *F Terry Davis *F > I look forward to hearing from you, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- *F > *F > *F > copy of mail you sent: *F > *F > From FlyBase-error@hedgehog.lbl.gov Thu Apr 06 13:57:56 2000 *F > Envelope-to: gm119@gen.cam.ac.uk *F > Delivery-date: Thu, 6 Apr 2000 13:57:56 \+0100 *F > From: FlyBase-error@hedgehog.lbl.gov *F > Date: Thu, 6 Apr 2000 06:02:26 \-0700 (PDT) *F > X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F > To: flybase-updates@morgan.harvard.edu *F > Subject: FlyBase error report *F > Content-Length: 1559 *F > *F > Error report from Terence Davis (davist2@cardiff.ac.uk) *F > Gene or accession: Accession number AE003722 genes CG7689 and CG6790 *F > Missed gene *F > Comments: I work on the fruitless gene and have cloned this gene *F > from several species. I have been examining the genome sequence *F > in comparison with the known cDNAs for D. melanogaster and *F > the known genome structure for D. heteroneura and D. silvestris. *F > (Both in Genbank af051662-af51670 and in press) *F > *F > The structure given in AE003722 is mostly OK except for the *F > 3'terminal exon and the 5' male specific peptide and exons. *F > *F > I have given below a detailed annotation based upon the *F > published D. melanogaster sequence data. *F > Coordinates are from AE003722 *F > complimentary strand. *F > *F > Accession number AF039231 is male specific fru (Ryner et al cell *F > 1996) *F > *F > Exons: *F > *F > 159917..159742 *F > 129972..129681 *F > (note start codon for male peptide 129961..129959) *F > 50339..50192 *F > 50119..49919 *F > 49611..48784 *F > 47367..47113 *F > 45513..45381 *F > 31849..31326 *F > (note stop codon 31828..31826) *F > *F > (note this is gene CG6790 fused to CG7689) *F > *F > Accession number U72492 is female fru sequence (Ryner et al *F > cell 1996) *F > *F > exons: *F > 159917..159742 *F > 129972..128080 *F > 50339..50192 *F > (note female start codon 50317..50315) *F > rest as for male seq above. *F > *F > *F > Accession number D84437 female fru (Ito et al. 1997 *F > PNAS) *F > *F > exons: *F > 50339..50192 *F > (note start codon 50317..50315) *F > 50119..49919 *F > 49611..48784 *F > 47367..47113 *F > 45513..45381 *F > 41715..40694 *F > (note stop codon 40696..40694) *F > (note that the sequence D84437 hReferer: *F > *F > Browser: Mozilla/2.02 (Win16; I) *F > Accessed from: FBupdate, /www/hedgehog_8000/htdocs *F Dr Terence Davis *F Department of Pathology *F University of Wales College of Medicine *F Heath Park *F Cardiff CF44XN *F Wales *F phone \+44-29-20742134 *F fax \+44-29-20744276 *F e.mail davist2@cardiff.ac.uk *F From wpttd@forest.cf.ac.uk Wed Jun 07 10:59:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Jun 2000 10:59:05 \+0100 *F From: 'Terence DAVIS' *F To: Gillian Millburn (Genetics) *F Date: Wed, 7 Jun 2000 11:03:55 GMT0BST *F MIME-Version: 1.0 *F Content-transfer-encoding: 7BIT *F Subject: Re: FlyBase error report for fru *F Priority: normal *F X-mailer: Pegasus Mail for Windows (v3.12) *F To: DavisT2@Cardiff.ac.uk *F Subject: Re: FlyBase error report for fru *F Copies to: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date sent: Wed, 7 Jun 2000 10:18:04 \+0100 *F > Dear Terry, *F > *F > thanks for the e-mail with the extra information about the various fru *F > splice forms. I will include it with your original error report as a *F > personal communication to FlyBase and it will be used by the molecular *F > curators at FlyBase to correct the annotation for fru so that all the *F > correct splice forms are shown. It will be some time before the gene *F > structure is corrected, as we are working through the genome region by *F > region systematically to refine all of the annotations. Thanks again *F > for sending this information, *F > *F > Gillian *F Please also note that I sent a correction for the gene CG7688. This *F is actually one of the terminal ends of the fru gene (Ryner et al *F sequence) and is only a single exon (bases 31849..31326) in my *F previous message. *F I will try to get permission to annotate the other splice variants as *F there are several more (these are as yet unpublished and are not *F my data). *F Terry *F Dr Terence Davis *F Department of Pathology *F University of Wales College of Medicine *F Heath Park *F Cardiff CF44XN *F Wales *F phone \+44-29-20742134 *F fax \+44-29-20744276 *F e.mail davist2@cardiff.ac.uk # *U FBrf0129156 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG2094 on Tue May 16 07:08:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 15:03:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 15:03:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 07:08:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2094 on Tue May 16 07:08:18 2000 *F Content-Length: 367 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2094 *F Gene annotation error *F Genes PTB and CG2094 should be merged. *F Comments: Sequence is identical for PTB (AF211191; AAF22979) and Celera *F CG2094 (AE003780; AAF57208), please merge the genes. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129157 *a Whitfield *b E. *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG2094 on Tue May 16 07:11:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 15:06:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 15:06:57 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 07:11:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2094 on Tue May 16 07:11:45 2000 *F Content-Length: 552 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2094 *F DNA error at position 59361. Upstream nucleotides: aagttcgtcgcttccgcgct *F Substitution: base g should be a. *F Comments: Sequence for PTB (AF211191; AAF22979) is a cDNA that starts *F translation *F at a Met located 13 amino acids upstream of the init Met in Celera *F CG2094 (AE003780; AAF57208). *F There may be a sequencing error that is hiding this upstream possible *F initiating Met. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129158 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG4371 on Wed May 17 07:52:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 15:47:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 15:47:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 07:52:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4371 on Wed May 17 07:52:41 2000 *F Content-Length: 531 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4371 *F Gene annotation error *F Genes GstD26 and CG4371 should be merged. *F Comments: The sequence of GstD26, Toung et al, J. Biol. Chem. *F 268:9737-9746(1993) (no *F accession number available as the TrEMBL entry Q9TX87 is derived from GenBank *F journal scan for peptide sequencing) and Celera CG4371 (AE003695; AAF54792) are *F the same so the genes should be merged. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129159 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG4181 on Wed May 17 08:22:44 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 16:17:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 16:17:57 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 08:22:44 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4181 on Wed May 17 08:22:44 2000 *F Content-Length: 530 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4181 *F Gene annotation error *F Genes GstD21 and CG4181 should be merged. *F Comments: The sequence of GstD21, Toung et al, J. Biol. Chem. *F 268:9737-9746(1993) (no *F accession number available as the TrEMBL entry Q9TX91 is derived from GenBank *F journal scan for peptide sequencing) and Celera CG4181 (AE003695; AAF54787) are *F the same so the genes should be merged. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129160 *a Robinson *b M. *t 2000.4.27 *T personal communication to FlyBase *u FlyBase error report for CG10986 on Thu Apr 27 06:22:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 14:18:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 14:18:09 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 06:22:46 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: msr12@mole.bio.cam.ac.uk *F Subject: FlyBase error report *F Content-Length: 593 *F Error report from Margaret Robinson (msr12@mole.bio.cam.ac.uk) *F Gene or accession: CG10986 *F Assembly error *F Comments: I believe that CG10986 and CG11197 are the same gene, garnet. At *F present, CG11197 is listed as garnet, but not CG10986. Neither of the deduced *F protein sequences, from either CG10986 or CG11197, are complete. Also, *F CG10986 was reported to map to 12A9 (although yesterday it said 12A2), CG11197 *F to 12A8, and garnet (g) to 12B6-7, so this also needs to be corrected. *F Browser: Mozilla/4.0 (compatible; MSIE 4.01; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 27 15:01:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Apr 2000 15:01:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 27 Apr 2000 07:01:38 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: msr12@mole.bio.cam.ac.uk *F Subject: FlyBase error report *F Content-Length: 955 *F Error report from Margaret Robinson (msr12@mole.bio.cam.ac.uk) *F Gene or accession: CG10986 *F Assembly error *F Comments: I just sent a message about this gene and now I need to update it. *F The reported sequence for the garnet gene product is in fact wrong; the one *F you want is AAC14585 (AP-3 delta-adaptin subunit from Drosophila) \- this is *F the correct garnet gene product sequence. It corresponds exactly to the *F CG10986 sequence at the N-terminal end, but then at amino acid 596 the two *F diverge, and the AAC14585 sequence goes on much longer. The CG11197 sequence *F starts at amino acid 274 of the CG10986 and AAC14585 sequences and follows the *F AAC14585 sequence. However, after the next-to-last amino acid of CG11197, *F which corresponds to amino acid 840 of AAC14585, the two diverge again, with *F an extra 200 or so amino acids added to AAC14585. *F Browser: Mozilla/4.0 (compatible; MSIE 4.01; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129161 *a Whitfield *b E. *t 2000.4.4 *T personal communication to FlyBase *u FlyBase error report on Tue Apr 4 13:21:22 2000. *F From eleanor@ebi.ac.uk Tue Apr 04 13:19:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 4 Apr 2000 13:19:09 \+0100 *F Date: Tue, 04 Apr 2000 13:21:22 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: Aubrey de Grey , Chihiro Yamada , *F 'Rachel Drysdale (Genetics)' , *F 'Gillian Millburn (Genetics)' , *F Michael Ashburner *F CC: Eleanor Whitfield *F Subject: cmg vs Caki *F Content-Transfer-Encoding: 7bit *F Hi All, *F . *F . *F . *F Celera cmg (AE003736; AAF55920.1) and Celera Caki (AE003736; AAF55922.1) do *F not show the same sequence similarities as the published sequence of each *F gene (U53190 and X94264) and using GeneScene there even appears to be a *F gene between them: CG13413. The sequence of this central gene does not *F align to either published sequence either. *F . *F . *F . *F The Celera and published sequence of each gene are quite different. *F For cmg the Celera gene aligns only to the C terminal fragment, *F frameshifts accounting for the later Init Met and for Caki the Celera *F gene aligns to the N terminus, frameshifts providing an early *F termination codon. *F . *F . *F . *F thanks *F Eleanor # *U FBrf0129162 *a Whitfield *b E. *t 2000.4.26 *T personal communication to FlyBase *u FlyBase error report on Wed Apr 26 13:30:01 2000. *F From eleanor@ebi.ac.uk Wed Apr 26 13:26:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 26 Apr 2000 13:26:22 \+0100 *F Date: Wed, 26 Apr 2000 13:30:01 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: ds: dachsous \- Celera annotation *F Content-Transfer-Encoding: 7bit *F Hi All, *F I am writing to flybase-help about this (on Aubreys advise) as the problem I *F have encountered may be at various levels of computational/manual annotation *F of the Celera sequence. *F dachsous cDNA has been published by a lab and sequence is available: *F SPTREMBL: Q24292 *F EMBL: L08811; AAA79329 *F Celera sequence (AE003588; AAF51468, CT39575) translates a 637 aa protein *F (SPTREMBL: Q9VPS4). The Celera initiating Met is 53aa upstream of the cDNA *F Met in the same frame, I believe this should be updated to reflect the *F experimentally proven cDNA data. Then the Celera splice site for the first *F intron is incorrect and alignment to the cDNA becomes very poor over the *F last 10 or so amino acids. The correct translation data for the remaining *F C-terminus is present in the CT39575 'jam' flat file page. The flat file *F translation provides a 3380 aa protein translation that overlaps with the *F 637 aa translation to make the correct translation as shown in Q24292. *F So if the N-terminus of AAF51468 CDS feature could be updated to start from *F the correct Met (MLRSSLL not MKATLDS) and the splice site positions for the *F first intron and remaining exons/introns is taken from the jam page then the *F translation for ds (CT39575) would be spot on! *F . *F . *F . *F many thanks *F Nellie # *U FBrf0129163 *a Ashburner *b M. *t 2000.4.26 *T personal communication to FlyBase *u FlyBase error report for CG10791 on Wed Apr 26 03:59:36 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 26 11:54:51 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 26 Apr 2000 11:54:51 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 26 Apr 2000 03:59:36 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report *F Content-Length: 295 *F Error report from M.Ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG10791 *F Missed gene *F Comments: I suspect that this is dunce, or part thereof, given (a) *F map position and (b) 'homology'. *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129164 *a Natzle *b J.E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG7116 on Thu May 4 13:34:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 21:29:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 21:29:52 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 13:34:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jenatzle@ucdavis.edu *F Subject: FlyBase error report *F Content-Length: 1000 *F Error report from Jeanette E. Natzle (jenatzle@ucdavis.edu) *F Gene or accession: CG7116 *F Gene annotation error *F Gene CG7116 corresponds to AF217281 (FBgn) *F Comments: We have isolated a gene (from cDNA and genomic clones) in 66C that *F we call IMP-E1. The genbank accession \# is AF217281. Based on our cDNA *F sequence the Gadfly CG7116 represents the middle and 3' portion of IMP-E1. *F CG13668 contains part of the 5' portion of the gene but has apparently *F 'translated' part of what is really intron regions in front and in back of the *F exon. IMP-E1 actually starts about 7 kb in front of CG13688 (the whole *F transcription unit is about 18.8 kb). I believe the start of transcription is *F at about nuc#242922 in genbank AE003555 of the Celera genomic scaffold and the *F gene ends at about nuc#224148. If you cannot get it to match up from the *F IMP-E1 genbank accession information, please let me know. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 98; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129165 *a Henikoff *b S. *t 2000.4.2 *T personal communication to FlyBase *u FlyBase error report for CG13329 on Sun Apr 2 10:50:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Apr 02 18:46:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 2 Apr 2000 18:46:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 2 Apr 2000 10:50:31 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report *F Content-Length: 660 *F Error report from Steve Henikoff (steveh@muller.fhcrc.org) *F Gene or accession: gb|AE003818.1|AE003818 Drosophila melanogaster genomic *F scaffold 142000013386047 *F section 28 of *F 52 *F Gene annotation error *F Gene CG13329 corresponds to AE003818 (FBgn0033843) *F Comments: The entry AC007452 is correct. See Henikoff et al (2000) PNAS *F 97:716-721 for *F molecular analysis of this gene (cid). The resulting suspect transcript *F structure *F ought to correct itself (beyond Asp38 of Cid) when the frameshift error is *F corrected, as cid is an intronless gene. *F Browser: Mozilla/4.7 en (X11; I; Linux 2.2.12-20 i686) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0129166 *a Butler *b H. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG10913 on Mon May 15 09:56:01 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 17:52:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 17:52:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 09:56:01 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hb246@gen.cam.ac.uk *F Subject: FlyBase error report for CG10913 on Mon May 15 09:56:01 2000 *F Content-Length: 307 *F Error report from Heather Butler (hb246@gen.cam.ac.uk) *F Gene or accession: CG10913 *F Gene annotation error *F Gene CG10913 corresponds to FBgn0028983 *F Comments: CG10913 is not Sema-5c, it is sp6 (FBgn0028983) *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.7 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129167 *a Butler *b H. *t 2000.5.15 *T personal communication to FlyBase *u FlyBase error report for CG5661 on Mon May 15 09:59:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 15 17:54:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 May 2000 17:54:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 May 2000 09:59:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hb246@gen.cam.ac *F Subject: FlyBase error report for CG5661 on Mon May 15 09:59:17 2000 *F Content-Length: 286 *F Error report from Heather Butler (hb246@gen.cam.ac) *F Gene or accession: CG5661 *F Gene annotation error *F Gene CG5661 corresponds to FBgn0028679 *F Comments: CG5661 is Sema-5c (FBgn0028679) *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.7 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129168 *a Whitfield *b E. *t 2000.5.17 *T personal communication to FlyBase *u FlyBase error report for CG13480 on Wed May 17 08:54:47 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed May 17 16:50:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 17 May 2000 16:50:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 17 May 2000 08:54:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG13480 on Wed May 17 08:54:47 2000 *F Content-Length: 1287 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG13480 *F Gene annotation error *F Genes CG13480 and Leucokinin should be merged. *F Comments: Amino acid and nucleotide sequence of Celera CG13480 (AE003534; *F AAF49731) and *F Leucokinin (AF192342; AAF20066) are 100% identical so please merge the genes. *F Leucokinin translation is just 31 amino acids but based on identity and *F same cytological location (70E) I believe these are the same genes. Clustal of *F C terminal CG13480 nucleotide sequence and complete Leucokinin is below. *F cel_xxxxx TTCTCGCCCATCATCCGGGATGCGGTGATTGAAAGGTGCCGCATCAAATCCCAGCTGCAG *F leuc_xxxxx \-------------------------TGATTGAAAGGTGCCGCATCAAATCCCAGCTGCAG *F cel_xxxxx CGCGACGAGAAGCGCAACTCCGTCGTGCTGGGCAAGAAGCAGCGATTCCACTCGTGGGGC *F leuc_xxxxx CGCGACGAGAAGCGCAACTCCGTCGTGCTGGGCAAGAAGCAGCGATTCCACTCGTGGGGC *F cel_xxxxx GGCAAAAGGTCACCGGAACCACCGATCCTGCCGGACTACTAA *F leuc_xxxxx GGCAAAAGGTCACCGGAACCACCGATCCTGCCGGACTACTAA *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129169 *a Ashburner *b M. *t 2000.5.29 *T personal communication to FlyBase *u FlyBase error report for CG11514 on Mon May 29 06:41:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 29 14:35:44 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 29 May 2000 14:35:44 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 29 May 2000 06:41:00 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.cuk *F Subject: FlyBase error report for CG11514 on Mon May 29 06:41:00 2000 *F Content-Length: 241 *F Error report from m.ashburner (ma11@gen.cam.ac.cuk) *F Gene or accession: CG11514 *F Missed gene *F Comments: This is a C-terminal exon of broad *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129170 *a Ashburner *b M. *t 2000.5.29 *T personal communication to FlyBase *u FlyBase error report for CG11509 on Mon May 29 06:38:47 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 29 14:33:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 29 May 2000 14:33:36 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 29 May 2000 06:38:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG11509 on Mon May 29 06:38:47 2000 *F Content-Length: 237 *F Error report from m.ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG11509 *F Missed gene *F Comments: This is a C-terminal part of br *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129171 *a Ashburner *b M. *t 2000.5.29 *T personal communication to FlyBase *u FlyBase error report for CG11511 on Mon May 29 06:39:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 29 14:34:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 29 May 2000 14:34:15 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 29 May 2000 06:39:31 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG11511 on Mon May 29 06:39:31 2000 *F Content-Length: 237 *F Error report from m.ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG11511 *F Missed gene *F Comments: This is a C-terminal exon of br *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129172 *a Benos *b T. *t 2000.5.31 *T personal communication to FlyBase *u FlyBase error report for CG4199 on Wed May 31 14:26:37 2000. *F From benos@ebi.ac.uk Wed May 31 14:21:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 May 2000 14:21:23 \+0100 *F Date: Wed, 31 May 2000 14:26:37 \+0100 (BST) *F From: Takis Benos *F To: flybase-updates@morgan.harvard.edu *F cc: Eleanor Whitfield *F Subject: Re: Error report (CG4199) *F MIME-Version: 1.0 *F Error report from Takis Benos (benos@ebi.ac.uk) *F Gene or accession: CG4199 *F Gene annotation error *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG4199 *F > Gene annotation error *F > Gene EG:22E5.5 has incorrect exon/intron structure. *F > Comments: Comparison to EDGP sequence (AL031765; CAA21128.1) reveals the *F Celera translation *F > is 113 amino acids too short at the N terminus. I have translated the DNA *F and found *F > the missing 2 exons that match exactly to the EDGP sequence (no coordinates, *F sorry). *F > Please update the CDS feature of AE003423; AAF45717.1. *F Comment: *F CG4199 has incorrect splice sites. *F EST AI134307 confirms correct structure of gene EG:22E5.5. *F Also, gene EG:22E5.5 contains gene EG:22E5.6 in one of its introns. Gene *F EG:22E5.6 is further supported by ESTs AA141447 and AA141446. *F Best, *F takis benos *F EDGP # *U FBrf0129173 *a Benos *b T. *t 2000.5.31 *T personal communication to FlyBase *u FlyBase error report for CG12598 on Wed May 31 14:27:41 2000. *F From benos@ebi.ac.uk Wed May 31 14:22:41 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 31 May 2000 14:22:41 \+0100 *F Date: Wed, 31 May 2000 14:27:41 \+0100 (BST) *F From: Takis Benos *F To: flybase-updates@morgan.harvard.edu *F Subject: Re: Error report (CG12598) *F MIME-Version: 1.0 *F Error report from Takis Benos (benos@ebi.ac.uk) *F Gene or accession: CG12598 *F Gene annotation error *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG12598 *F > Gene annotation error *F > Gene EG:BACN35H14.1 has incorrect exon/intron structure. *F > Comments: Comparison to EDGP sequence (AL035207; CAA22774.1) reveals the *F Celera translation *F > is 21 amino acids too short at the N terminus. I have translated the DNA *F and found *F > the missing exon that matches exactly to the EDGP sequence (no coordinates, *F sorry). *F > Please update the CDS feature of AE003422; AAF45665.1. *F Comments: *F Gene CG12598 has incorrect splicing structure. *F Gene EG:BACN35H14.1 follows the structure of identical EST hit AA978800. *F This hit also extends 5' of the reported EG:BACN35H14.1, but in a region *F with stop codons in all three frames and no adequate start codon/splice *F site. Thus, i conclude that this is the 5' UTR of the gene (EDGP doesn't *F report UTRs). *F Best regards, *F takis benos *F EDGP # *U FBrf0129174 *a Crabtree *b J. *t 2000.5.20 *T personal communication to FlyBase *u FlyBase error report for CG11172 on Sat May 20 15:52:22 2000. *F From hf.grc@forsythe.stanford.edu Sat May 20 23:49:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 20 May 2000 23:49:37 \+0100 *F Mime-Version: 1.0 *F X-Sender: crabtree@cmgm.stanford.edu *F Date: Sat, 20 May 2000 15:52:22 \-0700 *F To: flybase-help@morgan.harvard.edu *F From: Jerry Crabtree *F The gene that you have identified as CG11172 is a homologue of a *F protein (TonEBP) identified as a regulator of the tonicity response by *F Kwon, Handler and colleagues several years ago. It binds to the *F regulatory elements of a large group of genes that are activated when *F cells are exposed in hyperosmotic solutions. TonEBP is distinquished *F from the NF-AT proteins in that they are not substrates for calcineurin, *F are not cytoplasmic and are not cyclosporin sensitive. However they do *F bind DNA with a similar rel domain. Outside of the rel domain they *F are have no similarity. Also TonEBP and CG11172 do not have the *F SP-repeats or the calcineurin binding sequence. The gene that you have *F found is likely to be a tonicity regulator and not a cell cycle regulator as *F you imply in your annotation. *F Gerald R. Crabtree *F Professor of Developmental Biology *F StanfordUniversity *F Stanford CA *F email crabtree@cmgm.stanford.edu *F Jerry Crabtree *F Rm B211 *F Stanford University School of Medicine *F 279 Campus Drive *F Stanford CA 94305-5323 *F phone 650 723 8391 *F fax 650 723 5158 *F Adminstrative Assistant, Jean Oberlindacher, 650 723 3685, *F hf.mjo@Forsythe.Stanford.EDU *F http://crablab.stanford.edu/ # *U FBrf0129175 *a Benos *b T. *t 2000.6.1 *T personal communication to FlyBase *u FlyBase error report on Thu Jun 1 01:44:41 2000. *F From benos@ebi.ac.uk Thu Jun 01 01:39:25 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Jun 2000 01:39:25 \+0100 *F Date: Thu, 1 Jun 2000 01:44:41 \+0100 (BST) *F From: Takis Benos *F To: Michael Ashburner *F cc: m.gatt@gen.cam.ac.uk, Eleanor Whitfield , *F flybase-updates@morgan.harvard.edu *F Subject: Re: Error reports *F MIME-Version: 1.0 *F EDGP's comments on some of the other corrections suggested by Nellie *F recently..... *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG13358 *F > Gene annotation error *F > Gene EG:34F3.10 has incorrect exon/intron structure. *F > Comments: EG:34F3.10 was isolated by EDGP: *F > AL031583; CAB41346.1 *F > They define a further 2 upstream exons so their encoded protein is 58 amino *F > acids longer. These exons are present in the Celera sequence and generate *F > an identical protein. Please amend the CDS feature of AE003418; AAF45553.1. *F It is not clear who are 'they', but...... *F Here are the two sequences. Clearly EDGP's EG:34F3.10 is longer (by two *F exons) from CG13358. Looking the whole genomic region, i couldn't find any *F reason why Celera excluded these two exons. Please, *F >EG:34F3.10 SPTREMBL:Q9XZT0 *F MPEVGWTPQKSMTKPNRRKSGSPAKPDLDLRQGPNYMDYLWYLAMELRAN *F RLHNTKSVMAGSVRATLLGLLMQILCNGLAGQQLLLTRPQRSLFETPLGH *F VEQVEQVPSLGWNSVGSGLGVGEWPYSPVGLGHMSKDELLTLLEAWKEVE *F EESATTTTASPADASTRRPPPPPLPPPPPPPPRPTPIPVSVAIPSPAVPP *F SGTPITVRLPAFVPVRLAAVFTPLAGGQADSAVPSANGVGGAGGDDLSTD *F DVTDDDGVSTRLFRFLPMPGARTRPQSNSQPRQQFVVRNTLDSVDAGTAP *F VRQQIKPEVQPKATAPPSIPKFPGHPTPFFRPRFGVPPSLANARFELVPS *F SQIIGDWRV *F >CG13358 SPTREMBL:Q9W5D1 *F MAGSVRATLLGLLMQILCNGLAGQQLLLTRPQRSLFETPLGHVEQVEQVP *F SLGWNSVGSGLGVGEWPYSPVGLGHMSKDELLTLLEAWKEVEEESATTTT *F ASPADASTRRPPPPPLPPPPPPPPRPTPIPVSVAIPSPAVPPSGTPITVR *F LPAFVPVRLAAVFTPLAGGQADSAVPSANGVGGAGGDDLSTDDVTDDDGV *F STRLFRFLPMPGARTRPQSNSQPRQQFVVRNTLDSVDAGTAPVRQQIKPE *F VQPKATAPPSIPKFPGHPTPFFRPRFGVPPSLANARFELVPSSQIIGDWR *F V *F > *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG14625 *F > Gene annotation error *F > Gene EG:BACR42I17.8 has incorrect exon/intron structure. *F > Comments: EG:BACR42I17.8 corresponds to CG14625 (known in FlyBase genes *F file, but *F > not in GadFly). *F > EG:BACR42I17.8 sequence (AL121806; CAB65885) is 18 amino acids longer *F > than CG14625 sequence (AE003420; AAF45592). The upstream aa are *F > present in an upstream exon so please amend the CDS feature (no *F > coordinates, sorry!). *F Again Celera's sequence is sorter with no evident reason. The structure i *F reported is a complete Genscan prediction. *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG14795 *F > Gene annotation error *F > Gene EG:196F3.3 has incorrect exon/intron structure. *F > Comments: Comparison to EDGP sequence (AL031024; CAA19831.1) reveals the *F Celera translation *F > is 12 amino acids too short at the N terminus. I have translated the DNA *F and found *F > the missing 2 exons that match exactly to the EDGP sequence. *F > Please update the CDS feature of AE003421; AAF45641.1. *F > FT CDS join(complement(51985..52090),complement(51737..51919), *F > FT complement(51620..51681)) *F > > *F > FT CDS join(complement(52173..52175),complement(51985..52123), *F > FT complement(51737..51919),complement(51620..51681)) *F > *F > please note mRNA feature will also need to be updated *F Again EDGP reported the longest prediction (this time was Genefinder), *F since there is no contradictory evidence. Genscan gave sorter gene *F prediction (probably the one reorted by Celera). *F > *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG3708 *F > Gene annotation error *F > Gene EG:BACR7A4.18 has incorrect exon/intron structure. *F > Comments: EG:BACR7A4.18 corresponds to CG3708 (known in FlyBase genes file, *F but *F > not in GadFly). *F > EG:BACR7A4.18 sequence (AL109630; CAB65874) is 13 amino acids longer *F > than CG3708 sequence (AE003419; AAF45565). The upstream aa are present *F > so please amend the CDS feature: *F > FT CDS complement(83014..84102) *F > > *F > FT CDS complement(83014..84141) *F Since this is a one exon prediction, i believe there is a frameshift *F somewhere. I cannot tell if it is EDGP's or Celera's though.... *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG3939 *F > Gene annotation error *F > Gene CG3939 has incorrect exon/intron structure. *F > Comments: CG3939 sequence (AE003426; AAF45851) is 12 amino acids shorter at *F the *F > N-terminus than EG:140G11.5 sequence (AL035395; CAA23057). These amino *F > acids are present in frame upstream of the Met defined by the CDS *F > feature. The coordinates of the longer CDS are those the mRNA feature *F > translates. *F That is strange. In this case both Genscan and Genfinder predictions *F agree. The prediction are further supported by EST matche(s). ACeDB shows *F two ATGs, with the first being 'stronger' (whatever that means for *F Genefinder). From what Nellie says, i assume that Celera probably chose *F the second ('weaker') one. Why? *F > Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F > Gene or accession: CG4406 *F > Gene annotation error *F > Gene EG:133E12.3 has incorrect exon/intron structure. *F > Comments: EG:133E12.3 was isolated by EDGP: *F > AL009192; CAA15687.1 *F > They define an upstream possible initiating Met so their encoded protein is 5 *F > amino acids longer. These codons are present in the Celera sequence and *F > generate an identical protein. Please amend the CDS feature of AE003422; *F > AAF45703.1. *F > FT CDS join(complement(243135..243709), *F > FT complement(242939..243056),complement(242776..242886), *F > FT complement(242550..242714)) *F > amend first exon to read: *F > complement(243135..243721) *F > *F Similar to the above case. Both Genefinder and Genscan agree to their *F prediction of choosing the first (stronger) ATG as start codon. Why did *F Celera report shorter gene? *F With my best regards, *F takis benos *F EDGP # *U FBrf0129176 *a Benos *b T. *c E. *d Whitfield *t 2000.6.2 *T personal communication to FlyBase *u FlyBase error report on Fri Jun 2 20:18:18 2000. *F From benos@ebi.ac.uk Fri Jun 02 20:13:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 2 Jun 2000 20:13:05 \+0100 *F Date: Fri, 2 Jun 2000 20:18:18 \+0100 (BST) *F From: Takis Benos *F To: Michael Ashburner *F cc: flybase-updates@morgan.harvard.edu, Eleanor Whitfield *F Subject: EG vs CG: some more... *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='0-371759018-959973498=:18695' *F Content-Length: 6565 *F Hello Michael and all: *F Here are some more cases you asked me to check: *F 1. EG:34F3.1 \+ EG:34F3.2 > CG12467 *F Comment: reported by Michael *F Takis : These genes are mere predictions. EDGP reported Genefinder *F predictions as such; Celera reported (presumambly) Genie ones. *F However, EG:34F3.1 contains motif PS00012 (PHOSPHOPANTETHEINE), whereas *F EG:34F3.2 contains PF00169 (PH domain) and PF00784 (domain in myosin *F and kinesin tails). Could the motifs in two genes be connected? (I *F don't know) *F 2. CG11381 \+ CG14624 compared to EG:BACR42I17.9 *F Comment: reported by Michael *F Takis : no evidence for either case. EDGP reports the longest gene. *F EDGP reports one gene that constitutes the intact Genefinder *F prediction. Celera reports two genes, presumambly two different *F Genie/Genscan predictions. EDGP also misses one exon at the 5' of the *F gene (reported by Celera). *F 3. CG18166 \+ CG18273 compared to EG:171D11.6 *F Comment: reported by Michael; Takis did the CLUSTAL analysis *F Takis : Celera's genes are wrongly reported. EDGP followed the *F predictions of Genscan and Genefinder as well as EST matches. Wherever *F the two predictions didn't agree, EDGP adapted the longest exon, if was *F agreeing with the EST hits. Based on that, the last exons were *F discarded, since the EST matches support presence of genes on the other *F strand. *F Most curiously, gene CG18166 seems to be part of gene CG18273, as it is *F shown in the alignment that is attached. *F All the best, *F t;) *F \------------------------------------------------------------------------------ *F -- *F CLUSTAL W (1.7) multiple sequence alignment *F >.............................................................................. *F ....... *F (truncatd part; Celera's genes do not match EG:171D11.6 *F >.............................................................................. *F ....... *F CG18166|FBan0018166|CT40990|FB *F \----------------------------------------MVRRSQEPEK *F CG18273|FBan0018273|CT41446|FB *F \-------------------------------------------------- *F EG_171D11.6 *F REEQKKRNESESSEKTKAEPKVDHKKKNRDPETAKIQELEDNGKQRQPKL *F CG18166|FBan0018166|CT40990|FB *F LLEENNSKTVRPLVTRLNYRDANATRLLNVALTHRQRLHLDPDEIEFVLS *F CG18273|FBan0018273|CT41446|FB *F \----------------LNYRDANATRLLNVALTHRQRLHLDPDEIEFVLS *F EG_171D11.6 *F IDENFRRICKIYIGHSLNYRDANATRLLNVALTHRQRLHLDPDEIEFVLS *F CG18166|FBan0018166|CT40990|FB *F SYWRQLNTDIEVGDTFASSALDCLEPAIKLILGYKTNEDFLLLLRRLSSQ *F CG18273|FBan0018273|CT41446|FB *F SYWRQLNTDIEVGDTFSSSALDCLEPAIKLIIGYKTNEDFLLLLHRLSSQ *F EG_171D11.6 *F SYWRQLNTDIEVGDTFSSSALDCLEPAIKLIIGYKTNEDFLLLLHRLSSQ *F CG18166|FBan0018166|CT40990|FB *F VDMLDVDIKHLISHGGSWQHRQPDCNSTTQ-------------------- *F CG18273|FBan0018273|CT41446|FB *F VEQMARPASQTEHTALQNVLTLLALFAKCSLSSVKGAMLNEHFEVISVSV *F EG_171D11.6 *F VEQMARPASQTEHTALQNVLTLLALFAKCSLSSVKGAMLNEHFEVISVSV *F \*: : .: . . :. . *F CG18166|FBan0018166|CT40990|FB *F \-------------------------------------------------- *F CG18273|FBan0018273|CT41446|FB *F ALRLPEPKDLAYSGHALRLLEAQRNLAGNRTVPLTGESLDCLLSSMLDID *F EG_171D11.6 *F ALRLPEPKDLAYSGHALRLLEAQRNLAGNRTVPLTGESLDCLLSSMLDID *F CG18166|FBan0018166|CT40990|FB *F \-------------------------------------------------- *F CG18273|FBan0018273|CT41446|FB *F IKHLISHGGSWQQFVDLYSALTDNLIVLLKQHSNLMSDRAAQLSVLCQDL *F EG_171D11.6 *F IKHLISHGGSWQQFVDLYSALTDNLIVLLKQHSNLMSDRAAQLSVLCQDL *F CG18166|FBan0018166|CT40990|FB *F \-------------------------------------------------- *F CG18273|FBan0018273|CT41446|FB *F IQAVVGYRAERKQTQDISETELDGLADLGLKLATVMATVRATQALAVKRV *F EG_171D11.6 *F IQAVVGYRAERKQTQDISETELDGLADLGLKLATVMATVRATQALAVKRV *F CG18166|FBan0018166|CT40990|FB *F \-------------------------------------------------- *F CG18273|FBan0018273|CT41446|FB *F APFLLIFTIRQMVATERPTTLFEKIKVHIVRVCHELIGICDHRAGHFILR *F EG_171D11.6 *F APFLLIFTIRQMVATERPTTLFEKVCFTLRERHWPSRSQNSSFAAFRRID *F CG18166|FBan0018166|CT40990|FB \----------------------------------- *F CG18273|FBan0018273|CT41446|FB SSNEAGARMYEGLVKDHEKYHKFRGKV-------- *F EG_171D11.6 QRFLFRVKKYMVLYSLRSNNCHFFILCVHVVYIFL *F \------------------------------------------------------------------------------ *F -- *F From eleanor@ebi.ac.uk Mon Jun 05 09:50:19 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 5 Jun 2000 09:50:19 \+0100 *F Date: Mon, 05 Jun 2000 09:50:29 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: Takis Benos *F CC: Michael Ashburner , flybase-updates@morgan.harvard.edu, *F Eleanor Whitfield *F Subject: Re: EG vs CG: some more... *F Content-Transfer-Encoding: 7bit *F Hi All, *F In response to Takis mail I only have one thing to add: *F >1. EG:34F3.1 \+ EG:34F3.2 > CG12467 *F > Comment: reported by Michael *F > Takis : These genes are mere predictions. EDGP reported Genefinder *F > predictions as such; Celera reported (presumambly) Genie ones. *F > However, EG:34F3.1 contains motif PS00012 (PHOSPHOPANTETHEINE), whereas *F > EG:34F3.2 contains PF00169 (PH domain) and PF00784 (domain in myosin *F > and kinesin tails). Could the motifs in two genes be connected? (I *F > don't know) *F In Swiss-prot there are no known genes that carry all three motifs mentioned *F above (apart from TrEMBL entry Q9W5D0 for CG12467!). Myosin proteins can *F carry ph domins so I suggest that this region remains annotated as two genes *F and CDS feature for CG12467 be split into two potential reading frames. *F thanks *F Nellie # *U FBrf0129177 *a Whitfield *b E. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report on Mon May 8 11:31:44 2000. *F From eleanor@ebi.ac.uk Mon May 08 11:27:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 11:27:16 \+0100 *F Date: Mon, 08 May 2000 11:31:44 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: 'Rachel Drysdale (Genetics)' , *F 'Gillian Millburn (Genetics)' , *F Chihiro Yamada *F CC: Aubrey de Grey , *F Michael Ashburner , *F Eleanor Whitfield *F Subject: EDGP gene merge: EG:EG0007.4 and EG:EG0007.12 *F Content-Transfer-Encoding: 7bit *F Hi All, *F Celera sequence: *F AE003430; AAF45924.1 *F has brought together the coding regions of two EDGP genes: *F EG:EG0007.4 *F EG:EG0007.12 *F into one CDS and and named the gene EG:EG0007.12. *F I guess these genes should be merged. I wrote to Takis about it the fact *F that Celera had merged the coding regions and he replied: *F >I'm afraid i don't *F >have any evidence to support one or the other. There are no strong *F >similarity hits and the EST hits are not continuing from one gene to the *F >other. I last annotated that clone in Jan.1999 and at the time i was using *F >only Genefinder. So i followed its predictions (due to lack of any other *F >supporting evidence). Celera used Genscan. Genefinder tends to split *F >genes; Genscan tends to connect them. There is not much to say. One *F >argument in favour of Celera's structure is that the two genes are very *F >close. One argument in support of EDGP structure is that there are only *F >weak splice sites in the area (according to the Genefinder's scoring *F >scheme, of course). *F I am taking Celera as the master sequence so I have to merge the sequences *F of the two proteins. Will you also be merging the genes? *F If yes, could you please tell me the symbol that will be valid? *F . *F . *F thanks *F Ele # *U FBrf0129178 *a Berg *b C. *t 2000.5.11 *T personal communication to FlyBase *u FlyBase error report for CG5067 on Thu May 11 13:21:03 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 11 21:16:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 11 May 2000 21:16:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 11 May 2000 13:21:03 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: berg@genetics.washington.edu *F Subject: FlyBase error report for CG5067 on Thu May 11 13:21:03 2000 *F Content-Length: 331 *F Error report from Celeste Berg (berg@genetics.washington.edu) *F Gene or accession: CG5067 *F Gene annotation error *F Gene CG5067 corresponds to FBgn0004884 *F Comments: bwk encodes four ovarian transcripts, one of which is represented by *F CG5067 *F Browser: Mozilla/4.08 en (Win95; I ;Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129179 *a Ashburner *b M. *c T. *d Benos *t 2000.5.16 *T personal communication to FlyBase *u FlyBase error report for CG14045 on Tue May 16 05:02:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 16 12:57:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 16 May 2000 12:57:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 May 2000 05:02:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG14045 on Tue May 16 05:02:16 2000 *F Content-Length: 653 *F Error report from m.ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG14045 *F Missed gene *F Comments: The translation of this CG matches that of EG:BACH7M4.1 (C-term of CG) *F and of EG:BACH7M4.2 (N-term of CG). Takis Benos thinks that the CG *F should be split: *F >From benos@ebi.ac.uk Sat May 06 19:40:05 2000 *F In this case, i must say that EDGP is right. There are two clear EST hits *F (not overlapping), one at the end of EG:BACH7M4.2 (AA141474) and one at the *F beginning of EG:BACH7M4.1 (AI533820). But EST AA141474 extends on the *F 3'UTR of EG:BACH7M4.2. *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129180 *a Ashburner *b M. *c T. *d Benos *t 2000.5.18 *T personal communication to FlyBase *u FlyBase error report for CG2766 on Thu May 18 06:30:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 18 14:26:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 May 2000 14:26:07 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 18 May 2000 06:30:59 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG2766 on Thu May 18 06:30:58 2000 *F Content-Length: 1325 *F Error report from m.ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG2766 *F Missed gene *F Comments: This CG should be merged with EG:BACN33B1.2 (aka CG2716). It was *F pointed out *F by Eleanor that CG2766 matches the N term of EG:BACN33B1.2 and CG2716 matches *F the C term. Takis looked at this with this reply: *F >From benos@ebi.ac.uk Wed May 17 16:55:58 2000 *F I had a look, indeed, at EG:BACN33B1.2 gene. Unfortunately, i don't have *F the Celera data on AceDB, but from what you say and from the other data i *F do have in ACeDB, it seems like Celera reported the predictions blindly. *F There are indeed two gene predictions in this area, which i connected *F together, based on EST hits. EG:BACN33B1.2 follows the splicing pattern of *F three perfect matched ESTs (AI106876, AI512291 and AA736166) and is based *F on Genscan and Genefinder predictions. The only exon that is not supported *F by ESTs is the last one; but it has good splice site, thus i included to *F the gene. According to the EST AI512291 there is at least one more exon at *F the upstream of EG:BACN33B1.2. However, i failed to find suitable ATG or *F splice site upstream; therefore, i declared it as UTR (but it is not *F reported; we never really stored 5'/3' UTRs in ACeDB). *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129181 *a Ashburner *b M. *t 2000.5.29 *T personal communication to FlyBase *u FlyBase error report for CG18503 on Mon May 29 14:25:07 2000. *F >From ma11@gen.cam.ac.uk Mon May 29 14:19:46 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Mon, 29 May 2000 14:19:46 \+0100 *F To: ag24@gen.cam.ac.uk *F Subject: gu *F Cc: ma11@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Mon, 29 May 2000 14:25:07 \+0100 *F Content-Length: 138 *F \*a CG18503 *F > *F \*a svr *F \# *F Looking at the EDGP annotation and Gadfly it is prtty *F clear that this CG is simply the alternative 5' exon of svr # *U FBrf0129182 *a Levis *b R. *t 2000.4.25 *T personal communication to FlyBase *u FlyBase error report for CG18352 on Tue Apr 25 00:08:40 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 25 08:03:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 25 Apr 2000 08:03:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 25 Apr 2000 00:08:40 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: rwlevis@syr.edu *F Subject: FlyBase error report *F Content-Length: 1443 *F Error report from Robert Levis (rwlevis@syr.edu) *F Gene or accession: CG18352 *F Gene annotation error *F Gene CG18352 has a mistake in the supporting evidence or functional assignment. *F Comments: This is not really a gene, it is a 5'-truncated copy of a novel *F transposon. According to the annotation, the limits of the gene in AE003546 *F are 62725-64364. The segment 62725-64380 is repeated with ~99% identity at at *F least 8 other sites in the genomic sequence, strongly suggesting that this is *F an interspered repetitive sequence. The putative protein CG18352 is the *F conceptual translation product of the reverse complement of 63507-64364. This *F sequence shows strong similarity to the predicted reverse transcriptase *F domain-containing proteins of a group of D. melanogaster non-LTR *F retrotransposons including jockey, BS, TART, Fex, Doc, and G. The similarity *F to the same group of retrotransposon proteins continues in the putative *F translation product of an overlapping ORF (the reverse complement of *F 62921-63622) of AE003546. Full-length copies of this jockey-like group of *F retrotransposons typically encode two ORFs, with the reverse transcriptase *F domain-containing ORF foun! *F d 3' of another ORF. From this I infer that the copy of the novel transposon *F in CG18352 is probably 5'-truncated, which is common for copies of non-LTR *F retrotransposons. *F Browser: Mozilla/4.72 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129183 *a Whitfield *b E. *t 2000.5.4 *T personal communication to FlyBase *u FlyBase error report for CG7689 on Thu May 4 08:39:12 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu May 04 16:34:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 May 2000 16:34:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 4 May 2000 08:39:12 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 2013 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7689 *F Gene annotation error *F Gene fru has incorrect exon/intron structure. *F Comments: fru turns out to be a complex alternatively spliced protein so I *F have tried to be *F as explicit as possible in this update \- I hope I have succeeded. *F Current Celera entry for fru: *F AE003722; AAF55564.1 *F is OK but annotation is missing 4 further isoforms. *F AE003722; AAF55564.1 translation starts at the female init Met (as defined by *F Ryner et al, Cell 87:1079-1089(1996) through to C terminal stop codon. *F Published cDNAs from Ryner et al *F AF039231; AAB96677.1 *F U72492; AAB92662.1 *F correspond to male and female isoforms with a different C terminus. This C *F terminus is present in the Celera sequence (didn't work out coordinates, sorry) *F so both isoforms should be annotated. *F At this stage there should be three isoforms. *F The male exon, as defined by Ryner et al, is present in Celera as a separate *F gene *F CG7690. This exon does not exist as a gene in its own right so CG7690 should be *F merged with fru and the CDS feature editted to splice the exon onto the CDS *F feature *F of Celera fru isoform (AE003722; AAF55564.1) *F CDS = complement(129673-129961) complement(50192-50339) ..then continue as is *F already *F annotated in the CDS feature. *F This CDS feature defines the intron and removes the stop codon of CG7690. *F At this stage there should be four isoforms. *F Finally, one further group isolated fru and defined a further isoform with a *F third *F unique C terminus. Again this translation is present in Celere sequence so *F should be *F annotated (again I have no coordinates), note there is one conflict in the *F translation *F between Celera and published sequence. *F The isoform sequence is in D84437; BAA12663.1, Ito et al, Proc. Natl. Acad. Sci. *F U.S.A. 93:9687-9692(1996). *F Now you should have 5 isoforms and I know of no further isoforms from the *F literature. *F thank you! *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129184 *a Chihara *b C.J. *t 2000.5.8 *T personal communication to FlyBase *u FlyBase error report for CG9370 on Mon May 8 11:49:40 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon May 08 19:44:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 19:44:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 11:49:40 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: chihara@usfca.edu *F Subject: FlyBase error report for CG9370 on Mon May 8 11:49:40 2000 *F Content-Length: 1209 *F Error report from Carol J. Chihara (chihara@usfca.edu) *F Gene or accession: CG9370 *F Gene annotation error *F Gene CG9370 has incorrect exon/intron structure. *F Comments: This gene is the sequence of the gene 'ome' already shown to be for *F DPPIV as reported in the last Drosophila Research Conference. *F The 5' end of the gene includes another exon at some distance from the exon *F shown in gadfly. It consists of 99 bases in AE03553.1 from 217416 to 217514. *F This sequence is found in your own sequence of LD21715 ) as well as in SD01802 *F 5'. According to the sequence of AE03553.1 there is thus a very large intron *F between exon 1 and 2 which goes from 217415 to 187760. This intron inculdes *F two possible genes according to Genescene, and overlaps with a possible gene *F at the exon 1 region named CG17705. *F These two ESTs are from different libraries and share the same intron-exon *F structure for the sequences regions. Thus I believe that the exon is real at *F the 5' end. There is a two base difference in the sequence between AE03353.1 *F and the EST sequence which is most probably a strain difference (or sequencing *F error). *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129185 *a Beckendorf *b S. *t 2000.5.9 *T personal communication to FlyBase *u FlyBase error report on Tue May 9 15:20:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue May 09 23:15:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 May 2000 23:15:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 9 May 2000 15:20:21 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: beckendo@uclink4.berkeley.edu *F Subject: FlyBase error report on Tue May 9 15:20:20 2000 *F Content-Length: 911 *F Error report from Steven Beckendorf (beckendo@uclink4.berkeley.edu) *F . *F . *F . *F Comments: CG7085 sequence is erroneously included in the Glued mRNA reported *F by Swaroop et al and included in the Genbank nr database. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129186 *a Millburn *b G. *t 2000.6.7 *T personal communication to FlyBase *u FlyBase error report for CG9842 on Wed Jun 7 13:52:09 2000. *F From gm119@gen.cam.ac.uk Wed Jun 07 13:46:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Jun 2000 13:46:58 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: possible scaffold assembly problem ? *F Content-Type: X-sun-attachment *F From: Gillian Millburn (Genetics) *F Date: Wed, 7 Jun 2000 13:52:09 \+0100 *F Content-Length: 66022 *F Hi All, *F I am trying to sort out an error report from Eleanor, see below: *F \------------------------------------------------------------------------------ *F --- *F >From FlyBase-error@hedgehog.lbl.gov Mon May 08 12:20:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 May 2000 12:20:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 May 2000 04:24:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report *F Content-Length: 1885 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9842 *F Gene annotation error *F Gene CG9842 corresponds to FBgn0005783 *F Comments: Currently CG9842 is under Pp12-14D, but I believe it should be under *F PpD33. *F Celera sequence of CG9942 AE003502; AAF48622.1 aligns 100% with genomic *F sequence of *F PpD33 S40008; AAB22469.1 *F d33_xxxxx \--------------------------------------------------------CCAG *F cele_xxxxx GAGAAGACGATGATCGATATTGAGGCGCCGGTGACGGTGTGCGGTGATATCCACGGCCAG *F d33_xxxxx TTCTACGACCTGATGAAGCTGTTCGAAGTGGGCGGCTCGCCCGCGAGCACCAAGTATCTG *F cele_xxxxx TTCTACGACCTGATGAAGCTGTTCGAAGTGGGCGGCTCGCCCGCGAGCACCAAGTATCTG *F d33_xxxxx TTCCTGGGC--------------------------------------------------- *F cele_xxxxx TTCCTGGGCGACTACGTCGATCGGGGCTACTTCAGCATCGAGTGCGTCCTGTATTTGTGG *F the alignment to Pp12-14D is not as good: *F 14d_xxxxx \--------------------------------------------------------CCAG *F cele_xxxxx GAGAAGACGATGATCGATATTGAGGCGCCGGTGACGGTGTGCGGTGATATCCACGGCCAG *F 14d_xxxxx TTCTACGATCTGATGAAGCTATTCGAGATTGGGNNCTCGCCGGCGACCACCAAGTATCTG *F cele_xxxxx TTCTACGACCTGATGAAGCTGTTCGAAGTGGGCGGCTCGCCCGCGAGCACCAAGTATCTG *F 14d_xxxxx TTCCTGGNN--------------------------------------------------- *F cele_xxxxx TTCCTGGGCGACTACGTCGATCGGGGCTACTTCAGCATCGAGTGCGTCCTGTATTTGTGG *F Maybe other evidence was used to asign CG9842 to Pp12-14D but based on *F sequence I think *F it should be under PpD33. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F \------------------------------------------------------------------------------ *F --- *F Note: Pp12-14D is a typo for Pp2B-14D (FBgn0011826) *F I tried sorting this out by aligning all the sequence accession numbers *F under PpD33 (FBgn0005783) and Pp2B-14D (FBgn0011826) to the genome *F sequence and seeing which (if any CGs) they correspond to. *F The results of some of these alignments are attached below *F ('alignments'). There is something complicated going on, which is too *F complicated for me to sort out without a nice graphical alignment *F tool. *F 1. parts of the sequence U30493 (Pp2B-14D RNA sequence, 4497bp) aligned *F to 2 regions on the same scaffold quite close to each other. *F basically the CDS of this RNA aligned to both coordinates *F 223979--222136 (the coordinates are going backwards cos the alignment *F is to the minus strand, just to make it harder to follow \!) and *F 232829--231243. These coordinates correspond roughly to CG9842 and *F CG9819 respectively. *F I got a similar result with the RNA sequence X77768 \- the CDS aligning *F to the above 2 regions. *F So perhaps these 2 RNAs are chimeric and there is a local duplication *F giving 2 protein phosphatases CG9842 and CG9819 ? *F However, given that the RNAs were submitted by 2 separate groups, *F perhaps the problem is with the assembly of the genomic sequence *F instead and there is no local duplication. *F Wasn't sure who needs to look into this (especially if the problem *F turns out to be in the assembly), hence I am sending this to *F flybase-updates (will curate this message as an error report so appears *F under Pp2B-14D and PpD33 for re-annotation). *F Gillian *F \------------------------------------------------------------------------------ *F --- *F Query= S40008 *F (73 letters) *F Database: Drosophila Genome *F 1181 sequences; 122,680,987 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaf... 145 1e-34 *F >gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaffold *F 142000013386053 section 19 of *F 30, complete sequence *F Length = 301929 *F Score = 145 bits (73), Expect = 1e-34 *F Identities = 73/73 (100%) *F Strand = Plus / Minus *F Query: 1 ccagttctacgacctgatgaagctgttcgaagtgggcggctcgcccgcgagcaccaagta 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223836 ccagttctacgacctgatgaagctgttcgaagtgggcggctcgcccgcgagcaccaagta *F 223777 *F Query: 61 tctgttcctgggc 73 *F ||||||||||||| *F Sbjct: 223776 tctgttcctgggc 223764 *F Score = 81.8 bits (41), Expect = 2e-15 *F Identities = 65/73 (89%) *F Strand = Plus / Minus *F Query: 1 ccagttctacgacctgatgaagctgttcgaagtgggcggctcgcccgcgagcaccaagta 60 *F |||||||||||| ||||||||||| ||||| | || |||||||| |||| ||||||||| *F Sbjct: 232404 ccagttctacgatctgatgaagctattcgagattgggggctcgccggcgaccaccaagta *F 232345 *F Query: 61 tctgttcctgggc 73 *F ||||||||||||| *F Sbjct: 232344 tctgttcctgggc 232332 *F Database: Drosophila Genome *F Posted date: Apr 10, 2000 4:44 PM *F Number of letters in database: 122,680,987 *F Number of sequences in database: 1181 *F Lambda K H *F 1.37 0.711 1.31 *F Gapped *F Lambda K H *F 1.37 0.711 1.31 *F Matrix: blastn matrix:1 \-3 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 2815 *F Number of Sequences: 1181 *F Number of extensions: 2815 *F Number of successful extensions: 734 *F Number of sequences better than 10.0: 17 *F length of query: 73 *F length of database: 122,680,987 *F effective HSP length: 17 *F effective length of query: 56 *F effective length of database: 122,660,910 *F effective search space: 6869010960 *F effective search space used: 6869010960 *F T: 0 *F A: 0 *F X1: 6 (11.9 bits) *F X2: 10 (19.8 bits) *F S1: 12 (24.3 bits) *F S2: 15 (30.2 bits) *F \------------------------------------------------------------------------------ *F -- *F Query= DM30493 (==U30493) *F (4497 letters) *F Database: Drosophila Genome *F 1181 sequences; 122,680,987 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaf... 3293 0.0 *F >gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaffold *F 142000013386053 section 19 of *F 30, complete sequence *F Length = 301929 *F Score = 3293 bits (1661), Expect = 0.0 *F Identities = 1805/1848 (97%), Gaps = 5/1848 (0%) *F Strand = Plus / Minus *F Query: 1157 ctcaagcagcacttcatcctggagggcaggatcgaggagagcg-ccgccctgcgcattat 1215 *F ||||||||||||||||| || |||||||| ||||||||| ||| |||||||| || || *F Sbjct: 223979 ctcaagcagcacttcattcttgagggcagaatcgaggag-gcgcccgccctgaaaatcat *F 223921 *F Query: 1216 ccaagagggcgccacgctattgcgcacggaaaagacgatgatcgacatcgaggcgccggt 1275 *F ||| || || ||| | || |||| ||| |||||||||||||| || ||||||||||| *F Sbjct: 223920 ccaggacggagccgccctgctgcgtcaggagaagacgatgatcgatattgaggcgccggt *F 223861 *F Query: 1276 gacggtgtgcggcgatatccatggccagttctacgatctgatgaagctattcgagattgg 1335 *F |||||||||||| |||||||| |||||||||||||| ||||||||||| ||||| | || *F Sbjct: 223860 gacggtgtgcggtgatatccacggccagttctacgacctgatgaagctgttcgaagtggg *F 223801 *F Query: 1336 gggctcgccggcgaccaccaagtatctgttcctgggcgactacgtcgatcggggctactt 1395 *F |||||||| |||| ||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223800 cggctcgcccgcgagcaccaagtatctgttcctgggcgactacgtcgatcggggctactt *F 223741 *F Query: 1396 cagcatcgagtgcgtcctgtatttgtggtcgctaaagatcacctatccgcagacgctgtt 1455 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223740 cagcatcgagtgcgtcctgtatttgtggtcgctaaagatcacctatccgcagacgctgtt *F 223681 *F Query: 1456 cctgctgcgcggcaaccacgagtgccggcacttaaccgagtacttcaccttcaagcagga 1515 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223680 cctgctgcgcggcaaccacgagtgccggcacttaaccgagtacttcaccttcaagcagga *F 223621 *F Query: 1516 gtgcaagatcaagtactcggagcgcgtgtacgacgcctgcatggacgcattcgactgcct 1575 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223620 gtgcaagatcaagtactcggagcgcgtgtacgacgcctgcatggacgcattcgactgcct *F 223561 *F Query: 1576 gccgctggcggcgctaatgaaccagcagttcctctgcgtgcacggcggtctgtcgccgga 1635 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223560 gccgctggcggcgctaatgaaccagcagttcctctgcgtgcacggcggtctgtcgccgga *F 223501 *F Query: 1636 gatacacgagctggaggacatccggcggctcgaccgcttcaaggagcctcccgcctttgg 1695 *F |||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| *F Sbjct: 223500 gatacacgagctggaggacatccggcggctcgaccgcttcaaggagccgcccgcctttgg *F 223441 *F Query: 1696 ccccatgtgcgacctgctgtggtccgatccgctagaggacttcggcaacgagaagaactc 1755 *F |||||||||||||||||||||||||||||| || |||||||| ||||||||||||||||| *F Sbjct: 223440 ccccatgtgcgacctgctgtggtccgatcccctggaggactttggcaacgagaagaactc *F 223381 *F Query: 1756 ggacttctacacgcacaactccgtgcgcggctgctcgtacttctacagctacgccgcctg 1815 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223380 ggacttctacacgcacaactccgtgcgcggctgctcgtacttctacagctacgccgcctg *F 223321 *F Query: 1816 ctgcgacttcctgcagaacaacaacctgctgtcgatcatccgggcgcacgaggcgcagga 1875 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223320 ctgcgacttcctgcagaacaacaacctgctgtcgatcatccgggcgcacgaggcgcagga *F 223261 *F Query: 1876 cgccggctaccgcatgtaccgcaagagccagaccaccggcttcccctcgctgatcaccat 1935 *F |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 223260 cgccggctaccgcatgtaccgcaaaagccagaccaccggcttcccctcgctgatcaccat *F 223201 *F Query: 1936 cttctcggcgcccaactatctcgacgtgtacaacaacaaggcggcggtgctgaagtacga 1995 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223200 cttctcggcgcccaactatctcgacgtgtacaacaacaaggcggcggtgctgaagtacga *F 223141 *F Query: 1996 gaacaacgtgatgaacatccggcaattcaactgctcgccgcacccgtactggctgcccaa 2055 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223140 gaacaacgtgatgaacatccggcaattcaactgctcgccgcacccgtactggctgcccaa *F 223081 *F Query: 2056 cttcatggacgtgttcacctggtcgctacccttcgtgggcgagaaggtcaccgagatgtt 2115 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223080 cttcatggacgtgttcacctggtcgctacccttcgtgggcgagaaggtcaccgagatgtt *F 223021 *F Query: 2116 ggtgaacgtgctgaacatctgctccgacgacgagctcatgaccgaggagagcgaggagcc 2175 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223020 ggtgaacgtgctgaacatctgctccgacgacgagctcatgaccgaggagagcgaggagcc *F 222961 *F Query: 2176 gctctccgacgacgaggcggcgctgcgcaaggaggtgatacgcaacaagatccgtgccat 2235 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222960 gctctccgacgacgaggcggcgctgcgcaaggaggtgatacgcaacaagatccgtgccat *F 222901 *F Query: 2236 tggcaagatggcgcgcgtcttctccgtgctgcgcgaggagtccgagtcggtgttgcagct 2295 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| *F Sbjct: 222900 tggcaagatggcgcgcgtcttctccgtgctgcgcgaggagtccgagtcggtgctgcagct *F 222841 *F Query: 2296 gaagggcctgacgcccacgggcgccctgcccctcggcgccctctccggcggcaagcagtc 2355 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222840 gaagggcctgacgcccacgggcgccctgcccctcggcgccctctccggcggcaagcagtc *F 222781 *F Query: 2356 gctcaagaacgccatgcagggcttctcgcccaatcacaagattacctcgttcgcggaggc 2415 *F ||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||| *F Sbjct: 222780 gctcaagaacgccatgcagggcttctcgcccaaccacaagattacctcgttcgcggaggc *F 222721 *F Query: 2416 caagggcctggatgccgtcaacgaacggatgccgccgcggcgggatcagccgcccacgcc 2475 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222720 caagggcctggatgccgtcaacgaacggatgccgccgcggcgggatcagccgcccacgcc *F 222661 *F Query: 2476 cagcgaggatccgaatcagcacagtcagcagggcggcaaaaatggggctggacatgggta 2535 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222660 cagcgaggatccgaatcagcacagtcagcagggcggcaaaaatggggctggacatgggta *F 222601 *F Query: 2536 agctgtgccatctagtgcggctgcaggacgaggatgcagatgcagatgcggcacggtgga 2595 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222600 agctgtgccatctagtgcggctgcaggacgaggatgcagatgcagatgcggcacggtgga *F 222541 *F Query: 2596 gtctggcgaggtctgaattggtctgaaggaggggctgagtggagcggagcggaggcaaca 2655 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222540 gtctggcgaggtctgaattggtctgaaggaggggctgagtggagcggagcggaggcaaca *F 222481 *F Query: 2656 caaagaacaccaaccgaacggaaccttaattaaacttaatgtggcagagcaacaacttaa 2715 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222480 caaagaacaccaaccgaacggaaccttaattaaacttaatgtggcagagcaacaacttaa *F 222421 *F Query: 2716 atactaaaaccaattctaattctaagtaattgtcataaccccctgcatttcgtatgtgat 2775 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222420 atactaaaaccaattctaattctaagtaattgtcataaccccctgcatttcgtatgtgat *F 222361 *F Query: 2776 cccgcaccagctccatgcccatgtctatgtccatggcccagagccttccaaaccgacctg 2835 *F ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| | *F Sbjct: 222360 cccgcaccagctccatgcccatgtctatgtccatggcccagagcc-tccaaaccgac--g *F 222304 *F Query: 2836 gcacggagcagagatttgtttttacgcaggcgcgcgcacattggatattagaattatgtg 2895 *F |||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 222303 gcacggagcagagatttgtttttacgcaggcgcgcgcacattggatattaaaattatgtg *F 222244 *F Query: 2896 tggcatactttggcgcgccggcccccaaaacggaaaattccctttcgcgatagaagaaaa 2955 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222243 tggcatactttggcgcgccggcccccaaaacggaaaattccctttcgcgatagaagaaaa *F 222184 *F Query: 2956 ccaacccataacgtaacccataacctagcttaagttgtaatgtataaa 3003 *F |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222183 ccaacccataacgtaacccataacctagcttaagttgtaatgtataaa 222136 *F Score = 3075 bits (1551), Expect = 0.0 *F Identities = 1578/1587 (99%) *F Strand = Plus / Minus *F Query: 875 aatcaaaaggccaatgtcaacaacacacacgacaacaagaatgccgcggcgacgacgggg 934 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232829 aatcaaaaggccaatgtcaacaacacacacgacaacaagaatgccgcggcgacgacgggg *F 232770 *F Query: 935 acagcggcgggatcgggatcaggcggagcggcaggctcggcgggcacacagcagcagggt 994 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232769 acagcggcgggatcgggatcaggcggagcggcaggctcggcgggcacacagcagcagggt *F 232710 *F Query: 995 cagggcggcactggaacctcgagcggtccatccagcccaaccaagcgcagcacaatatcg 1054 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232709 cagggcggcactggaacctcgagcggtccatccagcccaaccaagcgcagcacaatatcg *F 232650 *F Query: 1055 acaaaggagcgtgtgatcgacagcgtggcatttccgccgagccgtaaactcacctgcgcg 1114 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232649 acaaaggagcgtgtgatcgacagcgtggcatttccgccgagccgtaaactcacctgcgcg *F 232590 *F Query: 1115 gatgtgttcgacgcgcggaccggaaagccgcagcacgatgtcctcaagcagcacttcatc 1174 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232589 gatgtgttcgacgcgcggaccggaaagccgcagcacgatgtcctcaagcagcacttcatc *F 232530 *F Query: 1175 ctggagggcaggatcgaggagagcgccgccctgcgcattatccaagagggcgccacgcta 1234 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232529 ctggagggcaggatcgaggagagcgccgccctgcgcattatccaagagggcgccacgcta *F 232470 *F Query: 1235 ttgcgcacggaaaagacgatgatcgacatcgaggcgccggtgacggtgtgcggcgatatc 1294 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232469 ttgcgcacggaaaagacgatgatcgacatcgaggcgccggtgacggtgtgcggcgatatc *F 232410 *F Query: 1295 catggccagttctacgatctgatgaagctattcgagattgggggctcgccggcgaccacc 1354 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232409 catggccagttctacgatctgatgaagctattcgagattgggggctcgccggcgaccacc *F 232350 *F Query: 1355 aagtatctgttcctgggcgactacgtcgatcggggctacttcagcatcgagtgcgtcctg 1414 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 232349 aagtatctgttcctgggcgactacgtcgatcggggctacttcagcatcgagtgcgtactg *F 232290 *F Query: 1415 tatttgtggtcgctaaagatcacctatccgcagacgctgttcctgctgcgcggcaaccac 1474 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232289 tatttgtggtcgctaaagatcacctatccgcagacgctgttcctgctgcgcggcaaccac *F 232230 *F Query: 1475 gagtgccggcacttaaccgagtacttcaccttcaagcaggagtgcaagatcaagtactcg 1534 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232229 gagtgccggcacttaaccgagtacttcaccttcaagcaggagtgcaagatcaagtactcg *F 232170 *F Query: 1535 gagcgcgtgtacgacgcctgcatggacgcattcgactgcctgccgctggcggcgctaatg 1594 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 232169 gagcgcgtgtacgacgcctgcatggacgcattcgactgcctgccgctggcggcgctcatg *F 232110 *F Query: 1595 aaccagcagttcctctgcgtgcacggcggtctgtcgccggagatacacgagctggaggac 1654 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232109 aaccagcagttcctctgcgtgcacggcggtctgtcgccggagatacacgagctggaggac *F 232050 *F Query: 1655 atccggcggctcgaccgcttcaaggagcctcccgcctttggccccatgtgcgacctgctg 1714 *F ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232049 atccgacggctcgaccgcttcaaggagcctcccgcctttggccccatgtgcgacctgctg *F 231990 *F Query: 1715 tggtccgatccgctagaggacttcggcaacgagaagaactcggacttctacacgcacaac 1774 *F |||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231989 tggtccgatccgctggaggacttcggcaacgagaagaactcggacttctacacgcacaac *F 231930 *F Query: 1775 tccgtgcgcggctgctcgtacttctacagctacgccgcctgctgcgacttcctgcagaac 1834 *F ||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231929 tccgtgcgcggttgctcgtacttctacagctacgccgcctgctgcgacttcctgcagaac *F 231870 *F Query: 1835 aacaacctgctgtcgatcatccgggcgcacgaggcgcaggacgccggctaccgcatgtac 1894 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231869 aacaacctgctgtcgatcatccgggcgcacgaggcgcaggacgccggctaccgcatgtac *F 231810 *F Query: 1895 cgcaagagccagaccaccggcttcccctcgctgatcaccatcttctcggcgcccaactat 1954 *F ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231809 cgcaaaagccagaccaccggcttcccctcgctgatcaccatcttctcggcgcccaactat *F 231750 *F Query: 1955 ctcgacgtgtacaacaacaaggcggcggtgctgaagtacgagaacaacgtgatgaacatc 2014 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231749 ctcgacgtgtacaacaacaaggcggcggtgctgaagtacgagaacaacgtgatgaacatc *F 231690 *F Query: 2015 cggcaattcaactgctcgccgcacccgtactggctgcccaacttcatggacgtgttcacc 2074 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231689 cggcaattcaactgctcgccgcacccgtactggctgcccaacttcatggacgtgttcacc *F 231630 *F Query: 2075 tggtcgctacccttcgtgggcgagaaggtcaccgagatgttggtgaacgtgctgaacatc 2134 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231629 tggtcgctacccttcgtgggcgagaaggtcaccgagatgttggtgaacgtgctgaacatc *F 231570 *F Query: 2135 tgctccgacgacgagctcatgaccgaggagagcgaggagccgctctccgacgacgaggcg 2194 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 231569 tgctccgacgacgagctcatgaccgaggagagcgaggagccactctccgacgacgaggcg *F 231510 *F Query: 2195 gcgctgcgcaaggaggtgatacgcaacaagatccgtgccattggcaagatggcgcgcgtc 2254 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231509 gcgctgcgcaaggaggtgatacgcaacaagatccgtgccattggcaagatggcgcgcgtc *F 231450 *F Query: 2255 ttctccgtgctgcgcgaggagtccgagtcggtgttgcagctgaagggcctgacgcccacg 2314 *F ||||||||||||||||||||||||||||||||| ||||||||||||| |||||||||||| *F Sbjct: 231449 ttctccgtgctgcgcgaggagtccgagtcggtgctgcagctgaagggactgacgcccacg *F 231390 *F Query: 2315 ggcgccctgcccctcggcgccctctccggcggcaagcagtcgctcaagaacgccatgcag 2374 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231389 ggcgccctgcccctcggcgccctctccggcggcaagcagtcgctcaagaacgccatgcag *F 231330 *F Query: 2375 ggcttctcgcccaatcacaagattacctcgttcgcggaggccaagggcctggatgccgtc 2434 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231329 ggcttctcgcccaatcacaagattacctcgttcgcggaggccaagggcctggatgccgtc *F 231270 *F Query: 2435 aacgaacggatgccgccgcggcgggat 2461 *F ||||||||||||||||||||||||||| *F Sbjct: 231269 aacgaacggatgccgccgcggcgggat 231243 *F Score = 1096 bits (553), Expect = 0.0 *F Identities = 553/553 (100%) *F Strand = Plus / Minus *F Query: 1 gtaattttcagttaacaatataaccgtgcattttttcttattatttacgaacctaagaag 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252351 gtaattttcagttaacaatataaccgtgcattttttcttattatttacgaacctaagaag *F 252292 *F Query: 61 tcgtgttttattgcatcgaattaaattacaatattataagtttttaggtttaaattactg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252291 tcgtgttttattgcatcgaattaaattacaatattataagtttttaggtttaaattactg *F 252232 *F Query: 121 atttgttaaaaatttcaatccgatttgaataaaactgctagttgctttttgttccatccc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252231 atttgttaaaaatttcaatccgatttgaataaaactgctagttgctttttgttccatccc *F 252172 *F Query: 181 tattatattatatccaactgagagttttcatatttccatacaccgcgaaacggtcacacc 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252171 tattatattatatccaactgagagttttcatatttccatacaccgcgaaacggtcacacc *F 252112 *F Query: 241 gcaccgcattcagtccaatcggaatcgctcctcgataagcccagacaaatcacgcctcta 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252111 gcaccgcattcagtccaatcggaatcgctcctcgataagcccagacaaatcacgcctcta *F 252052 *F Query: 301 tcgatttcttataagaaaggaaaacgaacgagcgtgttatcgagtgcaacatttgcgatt 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252051 tcgatttcttataagaaaggaaaacgaacgagcgtgttatcgagtgcaacatttgcgatt *F 251992 *F Query: 361 tgccctgctgcgcgcgtgcactttttattaatctcccgtataaatcagatatccgtgtgc 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 251991 tgccctgctgcgcgcgtgcactttttattaatctcccgtataaatcagatatccgtgtgc *F 251932 *F Query: 421 atttcgaacgcgctccgtcaacacacacacaaaaattaagtaaaaagtttaacacgaatt 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 251931 atttcgaacgcgctccgtcaacacacacacaaaaattaagtaaaaagtttaacacgaatt *F 251872 *F Query: 481 gcgaatagcgaaaaaacaaaagcttaaaccgtcgagtgtgaaaatttcgagctaaaagca 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 251871 gcgaatagcgaaaaaacaaaagcttaaaccgtcgagtgtgaaaatttcgagctaaaagca *F 251812 *F Query: 541 aaacgaagttaag 553 *F ||||||||||||| *F Sbjct: 251811 aaacgaagttaag 251799 *F Score = 884 bits (446), Expect = 0.0 *F Identities = 464/470 (98%) *F Strand = Plus / Minus *F Query: 3002 aaatgggcaaattttaaaatggaaaatgaacaaaacggaaagagctaattgaaatccctc 3061 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222125 aaatgggcaaattttaaaatggaaaatgaacaaaacggaaagagctaattgaaatccctc *F 222066 *F Query: 3062 cccctgcgataatgcgataatttaagatgaaaaaccccaacagaatccccataatcccca 3121 *F ||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 222065 cccctgcgataatgcgataatttaagatgaaaaaccccagcagaatccccataatcccca *F 222006 *F Query: 3122 aaggcaagaaacactaatccaattgaagagatatgggaaacaaaatccagcgctaatctg 3181 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222005 gaggcaagaaacactaatccaattgaagagatatgggaaacaaaatccagcgctaatctg *F 221946 *F Query: 3182 ttccaaacatttagcagcgccaaaaaagaagcgtacatatatgtatacatatgattattt 3241 *F ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221945 ttccaaacatttagcagcggcaaaaaagaagcgtacatatatgtatacatatgattattt *F 221886 *F Query: 3242 agaaatatcgaggcgcagtaccgagatacaaccgaaagtgtaatgaaatttgaaatgagc 3301 *F ||||||||||||||||||| ||||||| |||||||||||||||||||||||||||||||| *F Sbjct: 221885 agaaatatcgaggcgcagtgccgagatgcaaccgaaagtgtaatgaaatttgaaatgagc *F 221826 *F Query: 3302 aaaggtcattttttgtaataatcgtaatccttgaactctagaaatgtaatcttgcataga 3361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221825 aaaggtcattttttgtaataatcgtaatccttgaactctagaaatgtaatcttgcataga *F 221766 *F Query: 3362 agaaacgaatgaaataagcaaacaaattcgaaaccatttgcaatatgtgccgcaatagtc 3421 *F |||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221765 agaaacgaatgaaacaagcaaacaaattcgaaaccatttgcaatatgtgccgcaatagtc *F 221706 *F Query: 3422 cataaatgttccttatacgaattaaacattgaaagtgaaatcagagttta 3471 *F |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221705 cataaatgttccttatacgaattaaacattgaaagtgaaatcagagttta 221656 *F Score = 741 bits (374), Expect = 0.0 *F Identities = 390/394 (98%), Gaps = 1/394 (0%) *F Strand = Plus / Minus *F Query: 3995 aaacttattttccttgacttagtatgagctaattgcaatgagattaagcgaatgagatgt 4054 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221137 aaacttattttccttgacttagtatgagctaattgcaatgagattaagcgaatgagatgt *F 221078 *F Query: 4055 atttaagtaagcataaggcatggaacacacgtgagaaacggatgagtttcggcgtttcag 4114 *F ||||||||||| ||||||||||| |||||| ||||||||||||||||||||||||||||| *F Sbjct: 221077 atttaagtaagtataaggcatggcacacacatgagaaacggatgagtttcggcgtttcag *F 221018 *F Query: 4115 atcaattcaatgataatgatggcaatcaagttctcaatttgcgcctacacctcttcccaa 4174 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221017 atcaattcaatgataatgatggcaatcaagttctcaatttgcgcctacacctcttcccaa *F 220958 *F Query: 4175 aaaccgatgatatcagcttctcctttatcactaatcacaaccactatcaatgcgaaatcg 4234 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 220957 aaaccgatgatatcagcttctcctttatcactaatcacaaccactatcaatgcgaaatc- *F 220899 *F Query: 4235 aaaagctataagatataccacatacatactcacgcaagaaatgccttcatcgagatcata 4294 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 220898 aaaagctataagatataccacatacatactcacgcaagaaatgccttcatcgagatcata *F 220839 *F Query: 4295 ttatccacgaaccaagaatattaacgctgtacttttgcttcgactcttcaaccaccaata 4354 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 220838 ttatccacgaaccaagaatattaacgctgtacttttgcttcgactcttcaaccaccaata *F 220779 *F Query: 4355 attgcattgaactctaatctaaatatttatacag 4388 *F |||||||||||||||||||||||||||||||||| *F Sbjct: 220778 attgcattgaactctaatctaaatatttatacag 220745 *F Score = 500 bits (252), Expect = e-139 *F Identities = 261/264 (98%) *F Strand = Plus / Minus *F Query: 552 agagcgcgtggaatagcaagcagtgagagaggggaataccacggacgaatcaggacatag 611 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 233152 agagcgcgtggaatagcaagcagtgagagaggggaataccacggacgaatcaggacatag *F 233093 *F Query: 612 acaaggacaaggagcaacagcagcagcagcatttggcggtggagcaggagctggcgcgtc 671 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 233092 acaaggacaaggagcaacagcagcagcagcatttggcggtggagcaggagctggcgcgtc *F 233033 *F Query: 672 tggagcagtgacacgtgatcaactcttacgggtgggaaggtcagcaaccagcagccagca 731 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 233032 tggagcagtgacacgtgatcaactcttacgggtgggaaggtcagcaaccagcagccagca *F 232973 *F Query: 732 gaagctccagaatcagcagcagtcaacagtcgcggacgcaggcggggcagtcggaggatc 791 *F ||||||||||||||||||||||||| |||| | ||||||||||||||||||||||||||| *F Sbjct: 232972 gaagctccagaatcagcagcagtcagcagttgaggacgcaggcggggcagtcggaggatc *F 232913 *F Query: 792 gggcaaccatgtcttcgccggcgg 815 *F |||||||||||||||||||||||| *F Sbjct: 232912 gggcaaccatgtcttcgccggcgg 232889 *F Score = 498 bits (251), Expect = e-139 *F Identities = 282/289 (97%), Gaps = 5/289 (1%) *F Strand = Plus / Minus *F Query: 3682 tccataagagatcacacaagatgataatcgttgtttgaggatcacagtctagctaattaa 3741 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221443 tccataagagatcacacaagatgataatcgttgtttgaggatcacagtctagctaattaa *F 221384 *F Query: 3742 taaattgatgaataggaatcgaatcgatttccattgatgtgaatcgattttgaacactga 3801 *F ||||||||||||||||| |||| ||||||||||||||||||||| |||||||||||||| *F Sbjct: 221383 taaattgatgaatagga-tcga-tcgatttccattgatgtgaatgcattttgaacactga *F 221326 *F Query: 3802 aagaacggctaatcgtttaccatttaaatagcaaatcaaatatttaactattaaattact 3861 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221325 aagaacgg---atcgtttaccatttaaatagcaaatcaaatatttaactattaaattact *F 221269 *F Query: 3862 gtgtaaacctaataacgtataatatatttctgcgtgtaaattgaactaaacgattaatta 3921 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221268 gtgtaaacctaataacgtataatatatttctgcgtgtaaattgaactaaacgattaatta *F 221209 *F Query: 3922 attaactaatttagcgaatcgatttttgttgagagagaaagagcgctag 3970 *F ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221208 attaactaatttagcgaatcgatttttgttgagagagaaagagcgctag 221160 *F Score = 240 bits (121), Expect = 2e-61 *F Identities = 127/129 (98%) *F Strand = Plus / Minus *F Query: 3484 aaatatatacttaaatatatgtatatgtattattgttgactaatctaacttttgacttaa 3543 *F |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 221642 aaatatatacttaaatatatgtatatgtattattgttgcataatctaacttttgacttaa *F 221583 *F Query: 3544 tatgttacattttacgtgtacgactgtgtgtgtataactaactatttgcgttttatcagt 3603 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221582 tatgttacattttacgtgtacgactgtgtgtgtataactaactatttgcgttttatcagt *F 221523 *F Query: 3604 agcttggcg 3612 *F ||||||||| *F Sbjct: 221522 agcttggcg 221514 *F Score = 168 bits (85), Expect = 7e-40 *F Identities = 85/85 (100%) *F Strand = Plus / Minus *F Query: 4413 ccgagttggcattggaaaacagttagttttagtgatctatgcacgtatcgtaacaatacc 4472 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 220724 ccgagttggcattggaaaacagttagttttagtgatctatgcacgtatcgtaacaatacc *F 220665 *F Query: 4473 aagattaattaaatcgtagctaaaa 4497 *F ||||||||||||||||||||||||| *F Sbjct: 220664 aagattaattaaatcgtagctaaaa 220640 *F Score = 38.2 bits (19), Expect = 1.8 *F Identities = 19/19 (100%) *F Strand = Plus / Minus *F Query: 623 gagcaacagcagcagcagc 641 *F ||||||||||||||||||| *F Sbjct: 232884 gagcaacagcagcagcagc 232866 *F Score = 38.2 bits (19), Expect = 1.8 *F Identities = 19/19 (100%) *F Strand = Plus / Plus *F Query: 624 agcaacagcagcagcagca 642 *F ||||||||||||||||||| *F Sbjct: 94404 agcaacagcagcagcagca 94422 *F Score = 36.2 bits (18), Expect = 7.1 *F Identities = 18/18 (100%) *F Strand = Plus / Plus *F Query: 625 gcaacagcagcagcagca 642 *F |||||||||||||||||| *F Sbjct: 3897 gcaacagcagcagcagca 3914 *F Score = 36.2 bits (18), Expect = 7.1 *F Identities = 18/18 (100%) *F Strand = Plus / Plus *F Query: 3500 atatgtatatgtattatt 3517 *F |||||||||||||||||| *F Sbjct: 78795 atatgtatatgtattatt 78812 *F Database: Drosophila Genome *F Posted date: Apr 10, 2000 4:44 PM *F Number of letters in database: 122,680,987 *F Number of sequences in database: 1181 *F Lambda K H *F 1.37 0.711 1.31 *F Gapped *F Lambda K H *F 1.37 0.711 1.31 *F Matrix: blastn matrix:1 \-3 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 436306 *F Number of Sequences: 1181 *F Number of extensions: 436306 *F Number of successful extensions: 49165 *F Number of sequences better than 10.0: 345 *F length of query: 4497 *F length of database: 122,680,987 *F effective HSP length: 20 *F effective length of query: 4477 *F effective length of database: 122,657,367 *F effective search space: 549137032059 *F effective search space used: 549137032059 *F T: 0 *F A: 0 *F X1: 6 (11.9 bits) *F X2: 10 (19.8 bits) *F S1: 12 (24.3 bits) *F S2: 18 (36.2 bits) *F \------------------------------------------------------------------------------ *F -- *F Query= DMPPP3C (==X77768) *F (3247 letters) *F Database: Drosophila Genome *F 1181 sequences; 122,680,987 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaf... 2081 0.0 *F >gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaffold *F 142000013386053 section 19 of *F 30, complete sequence *F Length = 301929 *F Score = 2081 bits (1050), Expect = 0.0 *F Identities = 1105/1121 (98%), Gaps = 3/1121 (0%) *F Strand = Plus / Minus *F Query: 373 gagagcgagagcgggagagcgagctagtg-aagtgggagcgggagaggagca--ccgatt 429 *F ||||||||||||||||||||||||||||| |||||||||||||||||||||| |||||| *F Sbjct: 224466 gagagcgagagcgggagagcgagctagtggaagtgggagcgggagaggagcagtccgatt *F 224407 *F Query: 430 ccgaatcccggaatagaaaccaggaaaaaggaaacgggaagcagtagccgtagaagcaga 489 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224406 ccgaatcccggaatagaaaccaggaaaaaggaaacgggaagcagtagccgtagaagcaga *F 224347 *F Query: 490 gccaaaaaccgaaacagaaacagaagtctcaaccatgtcttcgaataaccagagcagcag 549 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224346 gccaaaaaccgaaacagaaacagaagtctcaaccatgtcttcgaataaccagagcagcag *F 224287 *F Query: 550 cgttgcccaggcggcaacaagtgcccgcaccgtcagtgccggatcggcggaagccacaga 609 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224286 cgttgcccaggcggcaacaagtgcccgcaccgtcagtgccggatcggcggaagccacaga *F 224227 *F Query: 610 tgccaatagtaccgcctcgaacaataacaataatagcagcagtaccgctgcagcgggcaa 669 *F |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 224226 tgccaatagtaccgcctcgaacaacaacaataatagcagcagtaccgctgcagcgggcaa *F 224167 *F Query: 670 caacagcgacaacagcagtcccaccactggaacgggcacaggagcgagcactggaaagct 729 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224166 caacagcgacaacagcagtcccaccactggaacgggcacaggagcgagcactggaaagct *F 224107 *F Query: 730 gcacggcggccacacggcagtaaacaccaaggaacgggtggtggacagcgttcccttccc 789 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224106 gcacggcggccacacggcagtaaacaccaaggaacgggtggtggacagcgttcccttccc *F 224047 *F Query: 790 gcccagccacaagctgaccttggcggaggtgttcgaccaacgcaccggcaagcccaacca 849 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224046 gcccagccacaagctgaccttggcggaggtgttcgaccaacgcaccggcaagcccaacca *F 223987 *F Query: 850 tgagctgctcaagcagcacttcattcttgagggcagaatcgaggaggcgcccgccttgaa 909 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||| *F Sbjct: 223986 tgagctgctcaagcagcacttcattcttgagggcagaatcgaggaggcgcccgccctgaa *F 223927 *F Query: 910 aatcatccaggaaggagccgccctgctgcgtcaggagaagacgatgatcgatattgaggc 969 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223926 aatcatccaggacggagccgccctgctgcgtcaggagaagacgatgatcgatattgaggc *F 223867 *F Query: 970 cccggtgacggtgtgcggtgatatccacggccagttctacgacctgatgaagctgttcga 1029 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223866 gccggtgacggtgtgcggtgatatccacggccagttctacgacctgatgaagctgttcga *F 223807 *F Query: 1030 agtgggcggctcgccgcaaagcaccaagtatctgttcctgggcgactacgtcgatcgggg 1089 *F ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223806 agtgggcggctcgcccgcgagcaccaagtatctgttcctgggcgactacgtcgatcgggg *F 223747 *F Query: 1090 ctacttcagcatcgagtgcgtcctgtatttgtggtcgctaaagatcacctatccgcagac 1149 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223746 ctacttcagcatcgagtgcgtcctgtatttgtggtcgctaaagatcacctatccgcagac *F 223687 *F Query: 1150 gctgttcctgctgcgcggcaaccacgagtgccggcacttaaccgagtacttcaccttcaa 1209 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223686 gctgttcctgctgcgcggcaaccacgagtgccggcacttaaccgagtacttcaccttcaa *F 223627 *F Query: 1210 gcaggagtgcaagatcaagtactcggagcgcgtgtacgacgcctgcatggacgcattcga 1269 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223626 gcaggagtgcaagatcaagtactcggagcgcgtgtacgacgcctgcatggacgcattcga *F 223567 *F Query: 1270 ctgcctgccgctggcggcgctaatgaaccagcagttcctctgcgtgcacggcggtctgtc 1329 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223566 ctgcctgccgctggcggcgctaatgaaccagcagttcctctgcgtgcacggcggtctgtc *F 223507 *F Query: 1330 gccggagatacacgagctggaggacatccggcggctggaccgcttcaaggagcctcccgc 1389 *F |||||||||||||||||||||||||||||||||||| ||||||||||||||||| ||||| *F Sbjct: 223506 gccggagatacacgagctggaggacatccggcggctcgaccgcttcaaggagccgcccgc *F 223447 *F Query: 1390 ctttggccccatgtgcgacctgctgtggtccgatccgctagaggacttcggcaacgagaa 1449 *F |||||||||||||||||||||||||||||||||||| || |||||||| ||||||||||| *F Sbjct: 223446 ctttggccccatgtgcgacctgctgtggtccgatcccctggaggactttggcaacgagaa *F 223387 *F Query: 1450 gaactcggacttctacacgcacaactccgtgcgcggctgct 1490 *F ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223386 gaactcggacttctacacgcacaactccgtgcgcggctgct 223346 *F Score = 1816 bits (916), Expect = 0.0 *F Identities = 1036/1071 (96%), Gaps = 20/1071 (1%) *F Strand = Plus / Minus *F Query: 1486 ctgctgctacttcctgcagaacaacaacctgctgtcgatcatccgggcgcacgaggcgca 1545 *F ||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223323 ctgctgcgacttcctgcagaacaacaacctgctgtcgatcatccgggcgcacgaggcgca *F 223264 *F Query: 1546 ggacgccggctaccgcatgtaccgcaagagccagaccaccggcttcccctcgctgatcac 1605 *F ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| *F Sbjct: 223263 ggacgccggctaccgcatgtaccgcaaaagccagaccaccggcttcccctcgctgatcac *F 223204 *F Query: 1606 catcttctcggcgcccaactatctcgacgtgtacaacaacaaggcggcggtgctgaagta 1665 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223203 catcttctcggcgcccaactatctcgacgtgtacaacaacaaggcggcggtgctgaagta *F 223144 *F Query: 1666 cgagaacaacgtgatgaacatccggcaattcaactgctcgccgcacccgatctggctgcc 1725 *F ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 223143 cgagaacaacgtgatgaacatccggcaattcaactgctcgccgcacccgtactggctgcc *F 223084 *F Query: 1726 caacttcatggacgtgttcacctggtcgctgcccttcgtgggcgagaaggtcaccgagat 1785 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 223083 caacttcatggacgtgttcacctggtcgctacccttcgtgggcgagaaggtcaccgagat *F 223024 *F Query: 1786 gttggtgaacgtgctgaacatctgctccgacgacgagctcatgaccgaggagagcgagga 1845 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 223023 gttggtgaacgtgctgaacatctgctccgacgacgagctcatgaccgaggagagcgagga *F 222964 *F Query: 1846 gccactctccgacgacgaggcggccgtgcgcaaggaggtgatacgcaacaagatccgtgc 1905 *F ||| |||||||||||||||||||| |||||||||||||||||||||||||||||||||| *F Sbjct: 222963 gccgctctccgacgacgaggcggcgctgcgcaaggaggtgatacgcaacaagatccgtgc *F 222904 *F Query: 1906 cattggcaagatggcgcgcgtcttctccgtgctgcgcgaggagtccgagtcggtgctgca 1965 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222903 cattggcaagatggcgcgcgtcttctccgtgctgcgcgaggagtccgagtcggtgctgca *F 222844 *F Query: 1966 g---aagggcctgacgcccacgggcgccctgcccctcggcgccctctccggcggcaagca 2022 *F | |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222843 gctgaagggcctgacgcccacgggcgccctgcccctcggcgccctctccggcggcaagca *F 222784 *F Query: 2023 gtcgctcaagaacgccatgcagggcttctcgcccaaccacaagattacctcgttcgcgga 2082 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222783 gtcgctcaagaacgccatgcagggcttctcgcccaaccacaagattacctcgttcgcgga *F 222724 *F Query: 2083 ggccaagggcctggatgccgtcaacgaacggatgccgccgcggcgggatcagccgcccac 2142 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222723 ggccaagggcctggatgccgtcaacgaacggatgccgccgcggcgggatcagccgcccac *F 222664 *F Query: 2143 gcccagcgaggatccgaatcagcacagtcagcagggcggcaaaaatggggctggacatgg 2202 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222663 gcccagcgaggatccgaatcagcacagtcagcagggcggcaaaaatggggctggacatgg *F 222604 *F Query: 2203 gtaagctgtgccatctagtgccatctagtgcggctgcaggacgaggatgcagatgcagat 2262 *F ||||||| ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222603 gtaagct----------gtgccatctagtgcggctgcaggacgaggatgcagatgcagat *F 222554 *F Query: 2263 gcggcacggtggagtctggcgaggtctgaattggtctgaaggaggggctgagtggagcgg 2322 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222553 gcggcacggtggagtctggcgaggtctgaattggtctgaaggaggggctgagtggagcgg *F 222494 *F Query: 2323 agc--aggcaacacaaagaacaccaaccgaa-gcaaccttaattaaacttaatgtggcag 2379 *F ||| |||||||||||||||||||||||||| | |||||||||||||||||||||||||| *F Sbjct: 222493 agcggaggcaacacaaagaacaccaaccgaacggaaccttaattaaacttaatgtggcag *F 222434 *F Query: 2380 agcaacaacttaaatactaaaaccaattct-attctaagtaattgtcataa-tttttgca 2437 *F |||||||||||||||||||||||||||||| |||||||||||||||||||| |||| *F Sbjct: 222433 agcaacaacttaaatactaaaaccaattctaattctaagtaattgtcataaccccctgca *F 222374 *F Query: 2438 tttcgtatgtcatcccgcaccagctccatgcccatgtctatgtccatggcccagagcctc 2497 *F |||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222373 tttcgtatgtgatcccgcaccagctccatgcccatgtctatgtccatggcccagagcctc *F 222314 *F Query: 2498 caaaccgacggcacggagcagagatt--gttttacgcaggcgcgcgcacat 2546 *F |||||||||||||||||||||||||| |||||||||||||||||||||| *F Sbjct: 222313 caaaccgacggcacggagcagagatttgtttttacgcaggcgcgcgcacat 222263 *F Score = 1187 bits (599), Expect = 0.0 *F Identities = 637/649 (98%), Gaps = 3/649 (0%) *F Strand = Plus / Minus *F Query: 1486 ctgctgctacttcctgcagaacaacaacctgctgtcgatcatccgggcgcacgaggcgca 1545 *F ||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231891 ctgctgcgacttcctgcagaacaacaacctgctgtcgatcatccgggcgcacgaggcgca *F 231832 *F Query: 1546 ggacgccggctaccgcatgtaccgcaagagccagaccaccggcttcccctcgctgatcac 1605 *F ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| *F Sbjct: 231831 ggacgccggctaccgcatgtaccgcaaaagccagaccaccggcttcccctcgctgatcac *F 231772 *F Query: 1606 catcttctcggcgcccaactatctcgacgtgtacaacaacaaggcggcggtgctgaagta 1665 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231771 catcttctcggcgcccaactatctcgacgtgtacaacaacaaggcggcggtgctgaagta *F 231712 *F Query: 1666 cgagaacaacgtgatgaacatccggcaattcaactgctcgccgcacccgatctggctgcc 1725 *F ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 231711 cgagaacaacgtgatgaacatccggcaattcaactgctcgccgcacccgtactggctgcc *F 231652 *F Query: 1726 caacttcatggacgtgttcacctggtcgctgcccttcgtgggcgagaaggtcaccgagat 1785 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 231651 caacttcatggacgtgttcacctggtcgctacccttcgtgggcgagaaggtcaccgagat *F 231592 *F Query: 1786 gttggtgaacgtgctgaacatctgctccgacgacgagctcatgaccgaggagagcgagga 1845 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231591 gttggtgaacgtgctgaacatctgctccgacgacgagctcatgaccgaggagagcgagga *F 231532 *F Query: 1846 gccactctccgacgacgaggcggccgtgcgcaaggaggtgatacgcaacaagatccgtgc 1905 *F |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||| *F Sbjct: 231531 gccactctccgacgacgaggcggcgctgcgcaaggaggtgatacgcaacaagatccgtgc *F 231472 *F Query: 1906 cattggcaagatggcgcgcgtcttctccgtgctgcgcgaggagtccgagtcggtgctgca 1965 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231471 cattggcaagatggcgcgcgtcttctccgtgctgcgcgaggagtccgagtcggtgctgca *F 231412 *F Query: 1966 \---gaagggcctgacgcccacgggcgccctgcccctcggcgccctctccggcggcaagca 2022 *F |||||| |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231411 gctgaagggactgacgcccacgggcgccctgcccctcggcgccctctccggcggcaagca *F 231352 *F Query: 2023 gtcgctcaagaacgccatgcagggcttctcgcccaaccacaagattacctcgttcgcgga 2082 *F |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| *F Sbjct: 231351 gtcgctcaagaacgccatgcagggcttctcgcccaatcacaagattacctcgttcgcgga *F 231292 *F Query: 2083 ggccaagggcctggatgccgtcaacgaacggatgccgccgcggcgggat 2131 *F ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231291 ggccaagggcctggatgccgtcaacgaacggatgccgccgcggcgggat 231243 *F Score = 912 bits (460), Expect = 0.0 *F Identities = 523/544 (96%) *F Strand = Plus / Minus *F Query: 947 aagacgatgatcgatattgaggccccggtgacggtgtgcggtgatatccacggccagttc 1006 *F |||||||||||||| || ||||| ||||||||||||||||| |||||||| ||||||||| *F Sbjct: 232457 aagacgatgatcgacatcgaggcgccggtgacggtgtgcggcgatatccatggccagttc *F 232398 *F Query: 1007 tacgacctgatgaagctgttcgaagtgggcggctcgccgcaaagcaccaagtatctgttc 1066 *F ||||| ||||||||||| ||||| | || ||||||||| | |||||||||||||||| *F Sbjct: 232397 tacgatctgatgaagctattcgagattgggggctcgccggcgaccaccaagtatctgttc *F 232338 *F Query: 1067 ctgggcgactacgtcgatcggggctacttcagcatcgagtgcgtcctgtatttgtggtcg 1126 *F |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 232337 ctgggcgactacgtcgatcggggctacttcagcatcgagtgcgtactgtatttgtggtcg *F 232278 *F Query: 1127 ctaaagatcacctatccgcagacgctgttcctgctgcgcggcaaccacgagtgccggcac 1186 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232277 ctaaagatcacctatccgcagacgctgttcctgctgcgcggcaaccacgagtgccggcac *F 232218 *F Query: 1187 ttaaccgagtacttcaccttcaagcaggagtgcaagatcaagtactcggagcgcgtgtac 1246 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232217 ttaaccgagtacttcaccttcaagcaggagtgcaagatcaagtactcggagcgcgtgtac *F 232158 *F Query: 1247 gacgcctgcatggacgcattcgactgcctgccgctggcggcgctaatgaaccagcagttc 1306 *F |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 232157 gacgcctgcatggacgcattcgactgcctgccgctggcggcgctcatgaaccagcagttc *F 232098 *F Query: 1307 ctctgcgtgcacggcggtctgtcgccggagatacacgagctggaggacatccggcggctg 1366 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 232097 ctctgcgtgcacggcggtctgtcgccggagatacacgagctggaggacatccgacggctc *F 232038 *F Query: 1367 gaccgcttcaaggagcctcccgcctttggccccatgtgcgacctgctgtggtccgatccg 1426 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 232037 gaccgcttcaaggagcctcccgcctttggccccatgtgcgacctgctgtggtccgatccg *F 231978 *F Query: 1427 ctagaggacttcggcaacgagaagaactcggacttctacacgcacaactccgtgcgcggc 1486 *F || |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231977 ctggaggacttcggcaacgagaagaactcggacttctacacgcacaactccgtgcgcggt *F 231918 *F Query: 1487 tgct 1490 *F |||| *F Sbjct: 231917 tgct 231914 *F Score = 547 bits (276), Expect = e-154 *F Identities = 310/319 (97%), Gaps = 3/319 (0%) *F Strand = Plus / Minus *F Query: 2884 gatatgggaaacaaaatccagcgctaatctgttccaaacatttagcagcgccaaaaaaga 2943 *F |||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 221976 gatatgggaaacaaaatccagcgctaatctgttccaaacatttagcagcggcaaaaaaga *F 221917 *F Query: 2944 agcgt-catatatgtatacatatcattatttagaaatatcgaggcgcagtgccgagatgc 3002 *F ||||| ||||||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 221916 agcgtacatatatgtatacatatgattatttagaaatatcgaggcgcagtgccgagatgc *F 221857 *F Query: 3003 aaccgaaagtgtaatgaaatttcaaatgatggcaaaggtgcatttttgtaataatcgtaa 3062 *F |||||||||||||||||||||| |||||| |||||||| |||||||||||||||||| *F Sbjct: 221856 aaccgaaagtgtaatgaaatttgaaatga--gcaaaggtcattttttgtaataatcgtaa *F 221799 *F Query: 3063 tccttgaactctagaaatgtaatcttgcatagaagaaacgaatgaaacaagcaaacaaat 3122 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221798 tccttgaactctagaaatgtaatcttgcatagaagaaacgaatgaaacaagcaaacaaat *F 221739 *F Query: 3123 tcgaaaccatttgcaatatgtgccgcaatagtccataaatgttccttatacgaattaaac 3182 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221738 tcgaaaccatttgcaatatgtgccgcaatagtccataaatgttccttatacgaattaaac *F 221679 *F Query: 3183 attgaaagtgaaatcagag 3201 *F ||||||||||||||||||| *F Sbjct: 221678 attgaaagtgaaatcagag 221660 *F Score = 402 bits (203), Expect = e-110 *F Identities = 222/226 (98%), Gaps = 3/226 (1%) *F Strand = Plus / Minus *F Query: 2 acgtctgcagggccgaagcgaaaaaagtagcaaatactgaaacagagtgactatgattgt 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224858 acgtctgcagggccgaagcgaaaaaagtagcaaatactgaaacagagtgactatgattgt *F 224799 *F Query: 62 tgttaagtgaaagcagcacttttggaaaacggctaggccagcggc--aggc-acggaaga 118 *F ||||||||||||||||||||||||||||||||||||||||||||| |||| |||||||| *F Sbjct: 224798 tgttaagtgaaagcagcacttttggaaaacggctaggccagcggcgcaggccacggaaga *F 224739 *F Query: 119 aagcagcggagtcagcgttccaaaatcaaagcaaagccagcgcaaagtaaaggaaagcga 178 *F |||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||| *F Sbjct: 224738 aagcagcggagtcagcgttccaaaatcaaagcaaagccagcggaaagtaaaggaaagcga *F 224679 *F Query: 179 atcaaagctggaaggcgataaagcggctcaccaggcactccaacaa 224 *F |||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224678 atcaaagctggaaggcgataaagcggctcaccaggcactccaacaa 224633 *F Score = 317 bits (160), Expect = 9e-85 *F Identities = 172/176 (97%) *F Strand = Plus / Minus *F Query: 2709 gggcaaattttaaaatggaaaatgaacaaaacggaaagagctaattgaaatccctccccc 2768 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222121 gggcaaattttaaaatggaaaatgaacaaaacggaaagagctaattgaaatccctccccc *F 222062 *F Query: 2769 tgcgataatgcgataatttaagatgaaaaaccccaacagaatccccataatccccagagg 2828 *F ||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||| *F Sbjct: 222061 tgcgataatgcgataatttaagatgaaaaaccccagcagaatccccataatccccagagg *F 222002 *F Query: 2829 caagaaacactaatccaattgaagagtattgggaaacaaaatccagcgctaatctg 2884 *F |||||||||||||||||||||||||| ||||||||||||||||||||||||||| *F Sbjct: 222001 caagaaacactaatccaattgaagagatatgggaaacaaaatccagcgctaatctg 221946 *F Score = 77.8 bits (39), Expect = 1e-12 *F Identities = 53/57 (92%), Gaps = 3/57 (5%) *F Strand = Plus / Minus *F Query: 2647 cgcgatagaagaa---ccacccataacgtaacccataacctagcttaagttgtaatg 2700 *F ||||||||||||| | ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 222198 cgcgatagaagaaaaccaacccataacgtaacccataacctagcttaagttgtaatg 222142 *F Score = 63.9 bits (32), Expect = 2e-08 *F Identities = 41/43 (95%), Gaps = 2/43 (4%) *F Strand = Plus / Minus *F Query: 355 gaaacagaactgagccgtgagag--cgagagcgggagagcgag 395 *F ||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 224500 gaaacagaactgagccgtgagagagcgagagcgggagagcgag 224458 *F Score = 54.0 bits (27), Expect = 2e-05 *F Identities = 36/39 (92%) *F Strand = Plus / Minus *F Query: 857 ctcaagcagcacttcattcttgagggcagaatcgaggag 895 *F ||||||||||||||||| || |||||||| ||||||||| *F Sbjct: 232547 ctcaagcagcacttcatcctggagggcaggatcgaggag 232509 *F \------------------------------------------------------------------------------ *F -- *F Query= S40058 *F (73 letters) *F Database: Drosophila Genome *F 1181 sequences; 122,680,987 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaf... 129 8e-30 *F >gb|AE003502.1|AE003502 Drosophila melanogaster genomic scaffold *F 142000013386053 section 19 of *F 30, complete sequence *F Length = 301929 *F Score = 129 bits (65), Expect = 8e-30 *F Identities = 69/71 (97%) *F Strand = Plus / Minus *F Query: 1 ccagttctacgatctgatgaagctattcgagattgggnnctcgccggcgaccaccaagta 60 *F ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| *F Sbjct: 232404 ccagttctacgatctgatgaagctattcgagattgggggctcgccggcgaccaccaagta *F 232345 *F Query: 61 tctgttcctgg 71 *F ||||||||||| *F Sbjct: 232344 tctgttcctgg 232334 *F Score = 65.9 bits (33), Expect = 1e-10 *F Identities = 61/71 (85%) *F Strand = Plus / Minus *F Query: 1 ccagttctacgatctgatgaagctattcgagattgggnnctcgccggcgaccaccaagta 60 *F |||||||||||| ||||||||||| ||||| | || |||||| |||| ||||||||| *F Sbjct: 223836 ccagttctacgacctgatgaagctgttcgaagtgggcggctcgcccgcgagcaccaagta *F 223777 *F Query: 61 tctgttcctgg 71 *F ||||||||||| *F Sbjct: 223776 tctgttcctgg 223766 *F Database: Drosophila Genome *F Posted date: Apr 10, 2000 4:44 PM *F Number of letters in database: 122,680,987 *F Number of sequences in database: 1181 *F Lambda K H *F 1.37 0.711 1.31 *F Gapped *F Lambda K H *F 1.37 0.711 1.31 *F Matrix: blastn matrix:1 \-3 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 2198 *F Number of Sequences: 1181 *F Number of extensions: 2198 *F Number of successful extensions: 508 *F Number of sequences better than 10.0: 9 *F length of query: 73 *F length of database: 122,680,987 *F effective HSP length: 17 *F effective length of query: 56 *F effective length of database: 122,660,910 *F effective search space: 6869010960 *F effective search space used: 6869010960 *F T: 0 *F A: 0 *F X1: 6 (11.9 bits) *F X2: 10 (19.8 bits) *F S1: 12 (24.3 bits) *F S2: 15 (30.2 bits) # *U FBrf0129187 *a Boulianne *b G. *t 2000.6.6 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue May 02 13:21:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 2 May 2000 13:21:12 \+0100 *F To: gboul@sickkids.on.ca *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 2 May 2000 13:26:09 \+0100 *F Content-Length: 941 *F Dear Dr. Boulianne, *F I am curating your abstract for FlyBase: *F Xu et al., 1999, Molec. Biol. Cell 10(Suppl.): 299a *F 'Identification of Ubisnap, a ubiquitously expressed alphaSNAP-binding *F protein from Drosophila.' *F In this you mention a gene that is new to FlyBase: 'Ubisnap'. *F Do you have a short symbol for this gene ? *F Also, do you have a map location for it or do you know which 'CG' in *F FlyBase it corresponds to ? It is nice if we can keep as many gene *F records as possible anchored to the genome. *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From gboul@sickkids.on.ca Tue Jun 06 21:38:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 6 Jun 2000 21:38:33 \+0100 *F Date: Tue, 6 Jun 2000 16:40:10 \-0400 (EDT) *F X-Sender: gboul@resunix.sickkids.on.ca *F Mime-Version: 1.0 *F To: Gillian Millburn (Genetics) *F From: gboul@sickkids.on.ca (Gabrielle Boulianne) *F Subject: Re: FlyBase query *F Gillian, *F After a very long delay I finally have the answers to your questions. *F 1. short symbol for gene: usnp *F 2. The map location is 60A3-4 and the CG is CG11173. *F Gabrielle *F Gabrielle L. Boulianne, Ph.D. *F Senior Scientist *F Program in Developmental Biology *F Hospital for Sick Children & *F Associate Professor *F Dept. Medical Genetics *F University of Toronto *F 555 University Avenue *F Toronto, Ontario *F Canada M5G 1X8 *F Ph: 416-813-8701 *F Fax: 416-813-5086 *F email: gboul@sickkids.on.ca # *U FBrf0129195 *a Palter *b K. *t 2000.6.12 *T personal communication to FlyBase *u *F From palter@astro.ocis.temple.edu Mon Jun 12 20:03:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Jun 2000 20:03:38 \+0100 *F X-Sender: palter@astro.ocis.temple.edu (Unverified) *F Mime-Version: 1.0 *F Date: Mon, 12 Jun 2000 15:08:59 \+0800 *F To: Gillian Millburn (Genetics) *F From: palter@astro.ocis.temple.edu (Karen Palter) *F Subject: Re: FlyBase query *F >Dear Dr. Palter, *F > *F >I am curating your abstract for FlyBase: *F > *F >Kim et al., Molec. Biol. Cell 10(Supplement): 461a *F > *F >'Cloning and characterization of two hexosaminidase genes from *F >Drosophila.' *F > *F >In this you mention 2 genes which are new to FlyBase \- 'DmHex1' and *F >'DmHex2'. *F > *F >The symbols Hex1 and Hex2 are already in use in FlyBase for 2 *F >Hexokinase genes, do you have another symbol to use as the valid symbol *F >for these 2 genes in FlyBase, for example 'Hexo1' and 'Hexo2' or *F >'Hexos1' and 'Hexos2'. *F > *F >Also, do you have a map location for them or do you know which 'CG' in *F >FlyBase they correspond to ? It is nice if we can keep as many gene *F >records as possible anchored to the genome. *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F Dear Ms. Millburn, *F I looked up our sequences for the two hexosaminidases and Hexo1 is found *F on AE003480 between 293850 and 295050 bp at location 64B6. The *F corresponding cDNA clone is GH07901. *F Hexo2 is found on AE003444 at 294,355 to 295,955 at location 8A2. This *F corresponds to cDNA GH11303. *F If I can be of more assistance let me know. *F Karen Palter *F Karen Palter *F Department of Biology *F Temple University *F 12th St. and Norris St. *F Philadelphia, PA 19122 *F Ph: 215 204-8845 *F fax: 215 204-6646 *F email: palter@astro.ocis.temple.edu # *U FBrf0129196 *a Tellam *b R. *t 2000.6.8 *T personal communication to FlyBase *u FlyBase error report for CG13311 on Wed Jun 7 19:43:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 08 03:38:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Jun 2000 03:38:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 7 Jun 2000 19:43:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: Ross.Tellam@tag.csiro.au *F Subject: FlyBase error report for CG13311 on Wed Jun 7 19:43:26 2000 *F Content-Length: 379 *F Error report from Ross Tellam (Ross.Tellam@tag.csiro.au) *F Gene or accession: CG13311 *F Missed gene *F Comments: This protein sequence is probably a peritrophic matrix structural *F protein *F and NOT an alkaline phosphatase as presently annotated. This conclusion *F can be reached by BLAST searches. *F Browser: Mozilla/4.7 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129197 *a Boehm *b S. *t 2000.6.8 *T personal communication to FlyBase *u FlyBase error report for CG18262 on Thu Jun 8 06:55:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 08 14:51:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Jun 2000 14:51:06 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 8 Jun 2000 06:55:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: boehm@mdc-berlin.de *F Subject: FlyBase error report for CG18262 on Thu Jun 8 06:55:58 2000 *F Content-Length: 441 *F Error report from Siegfried Boehm (boehm@mdc-berlin.de) *F Gene or accession: CG18262 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG18262. *F Comments: CG18262 is a C2H2 zinc finger protein (ZFP) with 5 tandem fingers, *F it is already correct annotated in the InterPro collection of Fly-ZFPs, *F Cheers *F Siegfried Boehm *F Browser: Mozilla/4.7 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129198 *a Lasko *b P. *t 2000.6.8 *T personal communication to FlyBase *u FlyBase error report for CG12058 on Thu Jun 8 09:07:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 08 17:01:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Jun 2000 17:01:58 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 8 Jun 2000 09:07:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: Paul_Lasko@maclan.mcgill.ca *F Subject: FlyBase error report for CG12058 on Thu Jun 8 09:07:20 2000 *F Content-Length: 317 *F Error report from Paul Lasko (Paul_Lasko@maclan.mcgill.ca) *F Gene or accession: CG12058 *F Gene annotation error *F Gene CG12058 corresponds to FBgn0005639 *F Comments: The ORFs encoded by mxc (FBgn0005639) and CG12058 are identical. *F Browser: Mozilla/4.5 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129199 *a Tellam *b R. *t 2000.6.9 *T personal communication to FlyBase *u FlyBase error report for CG2666 on Thu Jun 8 23:11:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jun 09 07:06:25 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 9 Jun 2000 07:06:25 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 8 Jun 2000 23:11:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: Ross.Tellam@tag.csiro.au *F Subject: FlyBase error report for CG2666 on Thu Jun 8 23:11:32 2000 *F Content-Length: 467 *F Error report from Ross Tellam (Ross.Tellam@tag.csiro.au) *F Gene or accession: CG2666 *F Assembly error *F Comments: This is the chitin synthase, DmCS-1. The full length mRNA *F sequence has been incorrectly assembled. There are a *F number of small introns in the genomic sequence *F some of which are in frame and incorrectly read as exons while *F other small exons have not been included. *F Browser: Mozilla/4.7 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129200 *a Flores *b C. *t 2000.6.9 *T personal communication to FlyBase *u FlyBase error report for CG6754 on Fri Jun 9 13:16:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jun 09 21:10:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 9 Jun 2000 21:10:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 9 Jun 2000 13:16:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ccflores@facstaff.wisc.edu *F Subject: FlyBase error report for CG6754 on Fri Jun 9 13:16:10 2000 *F Content-Length: 262 *F Error report from Carlos Flores (ccflores@facstaff.wisc.edu) *F Gene or accession: CG6754 *F Missed gene *F Comments: This predicted gene is the same as 'nbs' FBgn0026198. *F Browser: Mozilla/4.07 (Macintosh; U; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129201 *a Sekelsky *b J. *t 2000.6.12 *T personal communication to FlyBase *u FlyBase error report for CG4549 and CG4555 on Mon Jun 12 07:37:48 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Jun 12 15:32:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Jun 2000 15:32:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 12 Jun 2000 07:37:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sekelsky@unc.edu *F Subject: FlyBase error report for CG4549 and CG4555 on Mon Jun 12 07:37:48 2000 *F Content-Length: 618 *F Error report from Jeff Sekelsky (sekelsky@unc.edu) *F Gene or accession: CG4549 and CG4555 *F Gene annotation error *F Gene CG4549 has incorrect exon/intron structure. *F Comments: We have sequence from one partial cDNA. Exons 1-4 are correctly *F assigned. *F The predicted exon 5 does not exist in our cDNA. Exons 5 and 6 should be: *F exon 5: 293,840 \- 293,962 *F exon 6: 297,105 \- >297,281 (predicted to be part of CG4555). *F The 3' end of exon 4, together with exons 5 and 6 encode a highly conserved *F kinase domain related to FRAP2, as indicated. *F Browser: Mozilla/4.72 en (Win98; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129202 *a Sekelsky *b J. *t 2000.6.12 *T personal communication to FlyBase *u FlyBase error report for CG7003 on Mon Jun 12 07:59:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Jun 12 15:54:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Jun 2000 15:54:37 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 12 Jun 2000 07:59:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sekelsky@unc.edu *F Subject: FlyBase error report for CG7003 on Mon Jun 12 07:59:46 2000 *F Content-Length: 736 *F Error report from Jeff Sekelsky (sekelsky@unc.edu) *F Gene or accession: CG7003 *F Gene annotation error *F Gene CG7003 has incorrect exon/intron structure. *F Comments: Predicted (left) and probable (right) exons are: *F exon 1: <59,131 \- 60,135 should be <59,131 \- 60,135 (correct) *F exon 2: 60,191 \- 60,520 should be 60,191 \- 60,520 (correct) *F exon 3: 60,585 \- 60,210 should be 60,585 \- 62,192 (incorrect 3') *F Two additional exons should be added: *F exon 4: should be 62,261 \- 62,750 *F exon 5: should be 62,807 \- >62,950 *F These are based on strong sequence similarities between CG7003 and Msh6; half *F of the ATPase domain was missing in the annotated version. *F Browser: Mozilla/4.72 en (Win98; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129205 *a Shulman *b J. *c K. *d McKim *t 2000.6.20 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Fri Jun 09 11:40:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 9 Jun 2000 11:40:11 \+0100 *F To: ds139@mole.bio.cam.ac.uk *F Subject: FlyBase query: par-1 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 9 Jun 2000 11:45:38 \+0100 *F Content-Length: 1593 *F Dear Daniel, *F I am curating your paper for FlyBase: *F Shulman et al., 2000, Cell 101(4): 377--388 *F and I have a couple of questions. *F 1. the lines 'l(2)k05603' and 'EP(2)1144'. *F which are stated to be in the par-1 locus in the Materials and Methods. *F My question is, are these two P-element insertions in the 5' UTR of the *F N1 transcription unit of par-1 ? (in the results it says that *F 'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 *F transcription unit' \- so I thought these 2 P-elements might be the *F other 2 that are not named). *F Also, do you know whether these P-elements affect the function of both *F par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' *F must affect both as we already have it as an allele of mei-W68, but I *F wanted to check. Is EP(2)1144 viable ? *F . *F . *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From jms78@hermes.cam.ac.uk Mon Jun 19 17:02:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Jun 2000 17:02:46 \+0100 *F User-Agent: Microsoft Outlook Express Macintosh Edition \- 5.01 (1630) *F Date: Mon, 19 Jun 2000 17:08:39 \+0100 *F Subject: FlyBase query: par-1 *F From: Joshua Shulman *F To: *F Mime-version: 1.0 *F Content-Type: multipart/alternative; *F boundary='MS_Mac_OE_3044279319_6650771_MIME_Part' *F Content-Length: 5535 *F Hi, *F daniel forwarded your questions to me. *F >1. the lines 'l(2)k05603' and 'EP(2)1144'. *F > *F >which are stated to be in the par-1 locus in the Materials and Methods. *F > *F >My question is, are these two P-element insertions in the 5' UTR of the *F >N1 transcription unit of par-1 ? (in the results it says that *F >'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 *F >transcription unit' \- so I thought these 2 P-elements might be the *F >other 2 that are not named). *F Your interpretation is correct--all three of these insertions are in the *F 5'UTR of the N1 transcription unit. I can't recall if this is stated in the *F paper, but you might want to make note of the fact that the original *F l(2)k06821 line, which I believe has been discarded public collections, was *F a double insert line--we recombined off the extraneous insert. *F > *F >Also, do you know whether these P-elements affect the function of both *F >par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' *F >must affect both as we already have it as an allele of mei-W68, but I *F >wanted to check. Is EP(2)1144 viable ? *F l(2)k05603 and EP(2)1144 are both viable, and weak, hypomorphic alleles of *F both genes. The mei-W68 allelism of 05603 was reported in McKim and *F Hayashi-Hagihara (1998) and for 1144 the allelism should be cited to Kim *F McKim (personal communication). *F . *F . *F take care, *F Josh *F \-- *F Joshua M Shulman *F Wellcome/CRC Institute *F Tennis Court Road *F Cambridge CB2 1QR *F England *F Tel: 44 1223 334-113 *F Fax: 44 1223 334-089 *F email: jms78@hermes.cam.ac.uk *F From gm119@gen.cam.ac.uk Tue Jun 20 11:35:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 20 Jun 2000 11:35:11 \+0100 *F To: mckim@rci.rutgers.edu *F Subject: FlyBase query: mei-W68 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 20 Jun 2000 11:40:50 \+0100 *F Content-Length: 9772 *F Dear Dr. McKim, *F I am writing to ask whether it is OK to include in FlyBase some *F information about 2 P-element insertion lines which I have obtained *F from Joshua Shulman, but which originally comes from you. *F I wrote to him about a couple of insertion lines from his paper: *F Shulman et al., 2000, Cell 101(4): 377--388 *F the lines are 'l(2)k05603' and 'EP(2)1144' which are in par-1 (and *F which it turns out also affect mei-W68) *F I wrote to ask him whether the 2 lines affect the function of both *F par-1 and mei-W68 or just one of the 2 genes, since the 2 genes share a *F promoter and 5' UTR. *F He replied that both lines affect both genes, but said that the *F information for the allelism of the 2 lines to mei-W68 should be cited *F as a personal communication from you, and that I should write and check *F that it it OK with you to include this information in FlyBase. *F If it is OK with you for this information to be included, then it seems *F to me that the simplest thing would be to include this information as a *F personal communication from both you and J. Shulman to FlyBase, *F I have included below the correspondence between me and Joshua for your *F information, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F here is the e-mail correspondence: *F >From gm119@gen.cam.ac.uk Fri Jun 09 11:40:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 9 Jun 2000 11:40:11 \+0100 *F To: ds139@mole.bio.cam.ac.uk *F Subject: FlyBase query: par-1 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 9 Jun 2000 11:45:38 \+0100 *F Content-Length: 1593 *F Dear Daniel, *F I am curating your paper for FlyBase: *F Shulman et al., 2000, Cell 101(4): 377--388 *F and I have a couple of questions. *F 1. the lines 'l(2)k05603' and 'EP(2)1144'. *F which are stated to be in the par-1 locus in the Materials and Methods. *F My question is, are these two P-element insertions in the 5' UTR of the *F N1 transcription unit of par-1 ? (in the results it says that *F 'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 *F transcription unit' \- so I thought these 2 P-elements might be the *F other 2 that are not named). *F Also, do you know whether these P-elements affect the function of both *F par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' *F must affect both as we already have it as an allele of mei-W68, but I *F wanted to check. Is EP(2)1144 viable ? *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F >From jms78@hermes.cam.ac.uk Mon Jun 19 17:02:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Jun 2000 17:02:46 \+0100 *F User-Agent: Microsoft Outlook Express Macintosh Edition \- 5.01 (1630) *F Date: Mon, 19 Jun 2000 17:08:39 \+0100 *F Subject: FlyBase query: par-1 *F From: Joshua Shulman *F To: *F Mime-version: 1.0 *F Content-Type: multipart/alternative; *F boundary='MS_Mac_OE_3044279319_6650771_MIME_Part' *F Content-Length: 5535 *F Hi, *F daniel forwarded your questions to me. *F >1. the lines 'l(2)k05603' and 'EP(2)1144'. *F > *F >which are stated to be in the par-1 locus in the Materials and Methods. *F > *F >My question is, are these two P-element insertions in the 5' UTR of the *F >N1 transcription unit of par-1 ? (in the results it says that *F >'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 *F >transcription unit' \- so I thought these 2 P-elements might be the *F >other 2 that are not named). *F Your interpretation is correct--all three of these insertions are in the *F 5'UTR of the N1 transcription unit. I can't recall if this is stated in the *F paper, but you might want to make note of the fact that the original *F l(2)k06821 line, which I believe has been discarded public collections, was *F a double insert line--we recombined off the extraneous insert. *F > *F >Also, do you know whether these P-elements affect the function of both *F >par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' *F >must affect both as we already have it as an allele of mei-W68, but I *F >wanted to check. Is EP(2)1144 viable ? *F l(2)k05603 and EP(2)1144 are both viable, and weak, hypomorphic alleles of *F both genes. The mei-W68 allelism of 05603 was reported in McKim and *F Hayashi-Hagihara (1998) and for 1144 the allelism should be cited to Kim *F McKim (personal communication). *F take care, *F Josh *F \-- *F Joshua M Shulman *F Wellcome/CRC Institute *F Tennis Court Road *F Cambridge CB2 1QR *F England *F Tel: 44 1223 334-113 *F Fax: 44 1223 334-089 *F email: jms78@hermes.cam.ac.uk *F >From gm119@gen.cam.ac.uk Tue Jun 20 09:44:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 20 Jun 2000 09:44:27 \+0100 *F To: jms78@hermes.cam.ac.uk *F Subject: Re: FlyBase query: par-1 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 20 Jun 2000 09:50:07 \+0100 *F Content-Length: 1615 *F Dear Josh, *F thanks for your reply to my questions. I would like to include the *F information about the various lines in the par-1 locus (the bit below) *F as a personal communication from you to FlyBase, as it is useful *F information. Is that OK with you ? *F Gillian *F > *F > >1. the lines 'l(2)k05603' and 'EP(2)1144'. *F > > *F > >which are stated to be in the par-1 locus in the Materials and Methods. *F > > *F > >My question is, are these two P-element insertions in the 5' UTR of the *F > >N1 transcription unit of par-1 ? (in the results it says that *F > >'l(2)k06821 is one of three P elements inserted in the 5' UTR of the N1 *F > >transcription unit' \- so I thought these 2 P-elements might be the *F > >other 2 that are not named). *F > *F > Your interpretation is correct--all three of these insertions are in the *F > 5'UTR of the N1 transcription unit. I can't recall if this is stated in the *F > paper, but you might want to make note of the fact that the original *F > l(2)k06821 line, which I believe has been discarded public collections, was *F > a double insert line--we recombined off the extraneous insert. *F > *F > > *F > >Also, do you know whether these P-elements affect the function of both *F > >par-1 and mei-W68 or just one of the genes ? I think that 'l(2)k05603' *F > >must affect both as we already have it as an allele of mei-W68, but I *F > >wanted to check. Is EP(2)1144 viable ? *F > *F > l(2)k05603 and EP(2)1144 are both viable, and weak, hypomorphic alleles of *F > both genes. The mei-W68 allelism of 05603 was reported in McKim and *F > Hayashi-Hagihara (1998) and for 1144 the allelism should be cited to Kim *F > McKim (personal communication). *F > *F >From jms78@hermes.cam.ac.uk Tue Jun 20 11:16:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 20 Jun 2000 11:16:57 \+0100 *F User-Agent: Microsoft Outlook Express Macintosh Edition \- 5.01 (1630) *F Date: Tue, 20 Jun 2000 11:22:53 \+0100 *F Subject: Re: FlyBase query: par-1 *F From: Joshua Shulman *F To: 'Gillian Millburn (Genetics)' *F Mime-version: 1.0 *F Content-transfer-encoding: 7bit *F Gillian, *F yes, this is of course, fine. But any reference to the mei-W68 allelism *F should be cited as personal communication from Kim McKim, you might want to *F check with him to get his permission directly, but I'm sure he wouldn't have *F a problem with this (he gave us permission to put all of this in the paper *F anyway). *F \--Josh *F . *F . *F . *F From mckim@rci.rutgers.edu Tue Jun 20 18:21:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 20 Jun 2000 18:21:50 \+0100 *F Date: Tue, 20 Jun 2000 13:27:33 \-0400 (EDT) *F From: Kim MCKIM *F X-Sender: mckim@amenti.rutgers.edu *F To: Gillian Millburn *F Subject: Re: FlyBase query: mei-W68 *F MIME-Version: 1.0 *F Gillian, *F In short, your plan sounds like a good way to handle the citation. *F The two insertions are hypomorphic alleles of mei-W68. *F Kim *F _____________________________________ *F Kim McKim, Ph.D. *F Waksman Institute, Rutgers University *F 190 Frelinghuysen RD *F Piscataway NY 08854 *F 908-445-1164 *F mckim@rci.rutgers.edu *F . *F . *F . # *U FBrf0129217 *a Mahaffey *b J. *t 2000.6.8 *T personal communication to FlyBase *u FlyBase error report for CG9223 on Thu Jun 8 09:46:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 08 17:41:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Jun 2000 17:41:36 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 8 Jun 2000 09:46:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jim_mahaffey@ncsu.edu *F Subject: FlyBase error report for CG9223 on Thu Jun 8 09:46:56 2000 *F Content-Length: 851 *F Error report from Jim Mahaffey (jim_mahaffey@ncsu.edu) *F Gene or accession: CG9223 *F Missed gene *F Comments: We have been working on genes in this region and have quite a bit of *F information to add concerning CG9223. This gene and CG9219 are parts of the *F same gene. We have submitted a publication describing the role of this gene, *F but it has not been excepted yet (and this could take a while as you are *F aware). We have the sequence of a large cDNA and some RACE data supporting our *F structure, but we do not have the complete mRNA sequence at this time. We *F have predicted the structure of the protein, and have sequence evidence for *F much of this. We call the gene disco-related. We also have phenotypic data *F that should go into flybase. How should we proceed? *F Browser: Mozilla/4.72 en (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129218 *a Whitfield *b E. *t 2000.6.22 *T personal communication to FlyBase *u FlyBase error report for CG4007 on Thu Jun 22 06:30:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 22 14:25:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Jun 2000 14:25:18 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Jun 2000 06:30:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4007 on Thu Jun 22 06:30:56 2000 *F Content-Length: 747 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4007 *F Gene annotation error *F Gene CG4007 has incorrect exon/intron structure. *F Comments: AF037164; AAD02091.1, genomic DNA submitted by Frith K.J. and Scott *F M., starts *F from an upstream initiating Met that is in-frame with current Celera defined *F init Met. *F Please update CDS and mRNA features of AE003819; AAF58420.1 to encode the *F longer translation: *F FT mRNA join(complement(172387..172707), *F > *F FT mRNA join(complement(172387..172755>), *F FT CDS join(complement(172387..172707), *F > *F FT CDS join(complement(172387..172755), *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129219 *a Whitfield *b E. *t 2000.6.22 *T personal communication to FlyBase *u FlyBase error report for CG3510 on Thu Jun 22 06:49:03 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 22 14:43:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Jun 2000 14:43:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Jun 2000 06:49:03 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3510 on Thu Jun 22 06:49:03 2000 *F Content-Length: 707 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3510 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG3510. *F Comments: Celera has annotated the longest isoform (isoform I): *F AE003458; AAF46904 *F Dalby and Glover, Development 115:989-997(1992), have identified two further *F isoforms: *F EMBL; AJ006773; CAA07239.1 *F EMBL; AJ006773; CAA07240.1 *F relative to the Celera sequence the isoform sequence changes are: *F 1-12: MVGTTLKMRGDE \-> MAALEK (IN ISOFORM II). *F 1-29: MISSING (IN ISOFORM III). *F Please add CDS and mRNA features for these 2 further isoforms *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129220 *a Whitfield *b E. *t 2000.6.22 *T personal communication to FlyBase *u FlyBase error report for CG5227 on Thu Jun 22 06:51:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 22 14:45:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Jun 2000 14:45:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Jun 2000 06:51:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5227 on Thu Jun 22 06:51:10 2000 *F Content-Length: 984 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5227 *F Gene annotation error *F Gene CG5227 has incorrect exon/intron structure. *F Comments: CDS feature of Celera sequence (AE003418; AAF45541) has a different *F splice *F site for the first exon/intron junction compared to EDGP annotation (AL132792; *F CAB65848) and sequence submission from Nguyen et al U88578; AAD09632.1. The *F differing splice sites means the length of the protein varies from 2221-2224 *F amino acids. *F EDGP MLKSAASSLRRRRPKTTITATLAIEMPSQPKLASLLAVLVLLCYCDSCFFCYADANLQQQ *F Nguyen MLKSAASSLRRRRPKTTITATLAIEMPSQPKLASLLAVLVLLCYCDSCFFCYADANLQQQ *F Celera MLKSAASSLRRRRPKTTITATLAIEMPSQPKLASLLAVLVLLCYCDSCFFY---ANLQQQ *F Could you please correct the splice site and amend the CDS and mRNA features. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129221 *a Whitfield *b E. *t 2000.6.22 *T personal communication to FlyBase *u FlyBase error report for CG18085 on Thu Jun 22 06:52:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 22 14:47:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Jun 2000 14:47:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Jun 2000 06:52:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18085 on Thu Jun 22 06:52:41 2000 *F Content-Length: 710 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18085 *F Gene annotation error *F Gene CG18085 has incorrect exon/intron structure. *F Comments: Celera sequence AE003484; AAF47992 defines the wrong initiating Met. *F There is a *F Met upstream and in frame of the present initiating Met that also falls within *F the mRNA feature. *F This upstream init Met is also supported by a cDNA sequenced by Bowtell et al, *F Genes Dev. 2:620-634(1988), X13666; CAB55310. *F Please amend the CDS feature: *F FT CDS join(complement(310884..311432), *F > *F FT CDS join(complement(310884..311564), *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129222 *a Whitfield *b E. *t 2000.6.22 *T personal communication to FlyBase *u FlyBase error report for CG5170 on Thu Jun 22 06:55:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 22 14:49:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Jun 2000 14:49:54 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Jun 2000 06:55:19 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5170 on Thu Jun 22 06:55:19 2000 *F Content-Length: 760 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5170 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG5170. *F Comments: Berkeley group defined a 1301 aa protein from this cDNA (AF132179; *F AAD34767). *F Celera annotation of this protein is a 1268 aa protein (AE003799; AAF57691). *F I think the difference is due to alternative splice site, Berkeley cDNA *F encoding an extra unique exon, *F . *F . *F Could you please look into this to decide upon alternative splicing or *F incorrect annotation. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129223 *a Whitfield *b E. *t 2000.6.29 *T personal communication to FlyBase *u FlyBase error report for CG8363 on Thu Jun 29 05:00:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 29 12:54:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 29 Jun 2000 12:54:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 29 Jun 2000 05:00:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG8363 on Thu Jun 29 05:00:22 2000 *F Content-Length: 996 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8363 *F Gene annotation error *F Gene CG8363 has incorrect exon/intron structure. *F Comments: Celera sequence for Paps (AE003515; AAF49102) has an incorrect *F splice site at *F the end of first intron beginning second exon junction. Error was noticed when *F the sequence was compared with a cDNA isolated and sequenced by Jullien et al, *F Mech. Dev. 68:179-186(1997) (Y12861; CAA73368). *F Please amend the CDS feature: *F FT CDS join(complement(202878..202922), *F FT complement(192940..193069),complement(191092..192617), *F FT complement(190821..191027)) *F > *F FT CDS join(complement(202878..202922), *F FT complement(192940..193054),complement(191092..192617), *F FT complement(190821..191027)) *F and also the corresponding position in the mRNA feature *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129226 *a Whitfield *b E. *t 2000.6.22 *T personal communication to FlyBase *u FlyBase error report for CG1862 on Thu Jun 22 08:28:47 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 22 16:23:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Jun 2000 16:23:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Jun 2000 08:28:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1862 on Thu Jun 22 08:28:47 2000 *F Content-Length: 863 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1862 *F Gene annotation error *F Gene CG1862 has incorrect exon/intron structure. *F Comments: Dai and Kunes submitted Ephrin sequence (AF216287; AAF28394.1) and *F this *F sequence differs from the Celera sequence (AE003843; AAF59334) by a *F contiguous stretch of amino acids. *F The difference appears to be in-frame with the last exon of the translation so *F I wonder if the Celera spice sites are correct? The Celera DNA translates to *F make an identical protein to that of Dai and Kune. Could you please check the *F splice sites? *F Also, intriguingly the Celera annotates 2 splice variants of Ephrin (alt 1 and *F 2) but the base location and translation is identical for both. Is there *F another isoform? *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129228 *a Huen *b D. *t 2000.6.29 *T personal communication to FlyBase *u FlyBase error report for CG14396 on Thu Jun 29 08:53:48 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jun 29 16:48:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 29 Jun 2000 16:48:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 29 Jun 2000 08:53:48 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: smh1008@cus.cam.ac.uk *F Subject: FlyBase error report for CG14396 on Thu Jun 29 08:53:48 2000 *F Content-Length: 582 *F Error report from David Huen (smh1008@cus.cam.ac.uk) *F Gene or accession: CG14396 *F cDNA or EST error *F Comments: cDNA sequences that span both genes can be found in:- *F AJ237970 DME237970 *F AJ237971 DME237971 *F AJ237972 DME237972 *F AJ237973 DME237973 *F The above are splice variants reported in Huen, Elsdon and Ponder (2000) 'The *F Drosophila Ret gene is transcribed in multiple alternatively spliced forms' *F Mol. Gen. Genet. in press. *F I have requested general release of these sequences. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129229 *a Bazinet *b C. *t 2000.7.10 *T personal communication to FlyBase *u FlyBase error report for CG7075 on Mon Jul 10 03:52:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Jul 10 11:47:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 10 Jul 2000 11:47:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 10 Jul 2000 03:52:54 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: bazinetc@stjohns.edu *F Subject: FlyBase error report for CG7075 on Mon Jul 10 03:52:53 2000 *F Content-Length: 760 *F Error report from Chris Bazinet (bazinetc@stjohns.edu) *F Gene or accession: CG7075 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG7075. *F Comments: This gene's product has been the subject of the Ph.D. thesis of Dora *F Bigler (1994) at the University of Zürich. By developmental Northern *F blotting, the gene is expressed only in the testes. Using antibodies prepared *F against a peptide from the predicted sequence, she showed that the protein *F appears to be localized to the nucleus in premeiotic spermatocytes, then in *F the nebenkern and mitochondrial derivatives of postmeiotic spermatocytes. No *F mutants have yet been identified. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129230 *a Whitfield *b E. *t 2000.7.12 *T personal communication to FlyBase *u FlyBase error report for CG4843 on Wed Jul 12 05:42:21 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Jul 12 13:36:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Jul 2000 13:36:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 12 Jul 2000 05:42:21 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4843 on Wed Jul 12 05:42:21 2000 *F Content-Length: 763 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4843 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG4843. *F Comments: Celera have annotated the thoracic isoform of Tm2 (AE003708; *F AAF55165.1), could *F you please also annotate the embryonic isoform. *F Both isoforms have been isolated by Basi and Storti, J. Biol. Chem. 261:817-827 *F (1986) and Karlik et al, Cell 37:469-481(1984) *F K03277; AAA28973 *F K03277; AAA28974 *F K02623; AAA28971 *F K02622; AAA28970 *F Relative to the thoracic isoform the sequence change is a C-terminal sequence *F change: *F 259-284: RLFNEKEKYKAICDDLDQTFAELTGY \-> ELGINKDRYKSLADEMDSTFAELAGY *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129231 *a Carthew *b R. *t 2000.7.12 *T personal communication to FlyBase *u FlyBase error report for CG9949 on Wed Jul 12 14:52:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Jul 12 22:47:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 12 Jul 2000 22:47:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 12 Jul 2000 14:52:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: carthew+@pitt.edu *F Subject: FlyBase error report for CG9949 on Wed Jul 12 14:52:54 2000 *F Content-Length: 488 *F Error report from Richard Carthew (carthew+@pitt.edu) *F Gene or accession: CG9949 *F Missed gene *F Comments: Function of gene product is in regulated ubiquitination of proteins *F to which sina directly or indirectly binds, resulting in their degradation by *F the proteasome. *F Sina product has no known sequence-specific DNA binding activity. *F see Li et al 1997 Cell 90, 469 and Tang et al 1997 Cell 90, 458 *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129232 *a Zhang *b Y. *t 2000.7.13 *T personal communication to FlyBase *u FlyBase error report for CG8014 on Thu Jul 13 14:37:36 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jul 13 22:31:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 13 Jul 2000 22:31:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Jul 2000 14:37:36 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: yz66@columbia.edu *F Subject: FlyBase error report for CG8014 on Thu Jul 13 14:37:36 2000 *F Content-Length: 533 *F Error report from Yinhua Zhang (yz66@columbia.edu) *F Gene or accession: CG8014 *F Gene annotation error *F Gene CG8014 has incorrect exon/intron structure. *F Comments: Exons appear to be predicted right, but the full predicted amino *F acid sequence is not available. Only amino acid sequence from the first two *F exons is available and the coding appears to end in the next intron. I have a *F prediction based on sequence homology to a C. elegans gene. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129233 *a Whitfield *b E. *t 2000.7.14 *T personal communication to FlyBase *u FlyBase error report for CG9167 on Fri Jul 14 02:15:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jul 14 10:09:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Jul 2000 10:09:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Jul 2000 02:15:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9167 on Fri Jul 14 02:15:27 2000 *F Content-Length: 1181 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9167 *F Gene annotation error *F Gene CG9167 has incorrect exon/intron structure. *F Comments: Celera sequence: AE003612; AAF52343 *F Translation is incorrect as annotation introduces as splice site that is not *F there. *F Please correct CDS feature (and mRNA) as shown *F FT CDS join(135762..135906,136657..137369,137455..137839, *F FT 137899..138524) *F > *F FT CDS join(135762..135906,136657..137369,137455..138524, *F FT 138900..139218) *F FT mRNA join(135093..135906,136657..137369,137455..137839, *F FT 137899..138815,138900..140157) *F > *F FT mRNA join(135093..135906,136657..137369,137455..138524, *F FT 138900..140157) *F translation then generates an 845 aa protein that is identical to that *F isolated by *F Pecasse et al, Dev. Biol. 221:53-67(2000) *F AJ005440; CAA06543 *F AJ005441; CAA06544 *F Please note they also annotate a second isoform that is missing in Celera. *F The isoform *F is lacking the N-terminal 54 amino acids. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129234 *a Whitfield *b E. *t 2000.7.14 *T personal communication to FlyBase *u FlyBase error report for CG6703 on Fri Jul 14 08:07:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jul 14 16:01:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 14 Jul 2000 16:01:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 14 Jul 2000 08:07:42 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6703 on Fri Jul 14 08:07:42 2000 *F Content-Length: 1184 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6703 *F Gene annotation error *F Gene CG6703 has incorrect exon/intron structure. *F Comments: CG6703 (cmg) and CG13412 (Caki) are the same gene. *F cmg cDNA is published by Dimitratos et al, Mech. Dev. 63:127-130(1997), *F sequence U53190; *F AAC80169 and Caki cDNA by Martin and Ollo, EMBO J. 15:1865-1876(1996), *F sequence X94264; *F CAA63940. X94264 has 2 frameshifts, but when corrected translation matches *F that of *F U53190. *F Therefore the Celera genes CG6703 and CG13412 should be merged to the same *F gene and the *F CDS features need to be amended to provide one translation of a 897aa protein. *F residues 1-444 of CG13412 (Caki) match the N-terminal 444aa of U53190 *F residues 41-461 of CG6703 (cmg) match the C terminal 477-897 residues of U53190 *F I cannot find the exon to encode the internal 444-477 residues. The reason *F for this is *F that when the cDNA maps to the genomic sequence it generates an internal *F intron in *F excess of 25,000 bp. *F Apologies for the complications of this update, I hope you find the missing *F translation. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129235 *a Whitfield *b E. *t 2000.7.17 *T personal communication to FlyBase *u FlyBase error report for CG15442 on Mon Jul 17 03:28:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Jul 17 11:22:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Jul 2000 11:22:46 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 17 Jul 2000 03:28:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG15442 on Mon Jul 17 03:28:45 2000 *F Content-Length: 780 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG15442 *F Gene annotation error *F Gene CG15442 has incorrect exon/intron structure. *F Comments: RpL27A translation in Celera (AE003576; AAF51006) is missing the *F N-terminal *F initiating Met. *F Genomic DNA provided in Soehnge et al, Gene 185:257-263(1997) (U66357; AAC47475) *F confirms this residue resides on an upstream exon. *F Please amend the CDS feature: *F FT CDS join(<158190..158507,158738..158866) *F > *F FT CDS join(157956..157958,158190..158507,158738..158866) *F also amend the mRNA feature to show this upstream exon, I do not know the 5' *F border of this exon so I cannot provide the update. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129236 *a Davis *b T. *t 2000.7.18 *T personal communication to FlyBase *u FlyBase error report on Tue Jul 18 10:55:24 2000. *F From wpttd@forest.cf.ac.uk Tue Jul 18 10:50:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Jul 2000 10:50:06 \+0100 *F From: 'Terence DAVIS' *F To: gm119@gen.cam.ac.uk *F Date: Tue, 18 Jul 2000 10:55:24 GMT0BST *F MIME-Version: 1.0 *F Content-transfer-encoding: 7BIT *F Subject: (Fwd) fruitless *F Priority: normal *F X-mailer: Pegasus Mail for Windows (v3.12) *F . *F . *F . *F Dear Gillian *F I have more information on possible fruitless alternative splice *F forms. *F Comparing melanogaster with the Hawaiian species heteroneura *F (AH009210 and silvestris (AH009214). *F There is an exon within the transcript CG7689 which is an *F alternative 3' end of fru in the Hawaiian species (see Gene *F 2462000 143-149). This sequence is conserved in melanogaster *F as follows: *F The exon is position 46583 to 45996 (complimentary AE003722) *F and would be spliced from position 47113. Stop codon is at 45998 *F to 45996 *F The additional peptide seq is attached below *F VKKSEAFLGSTGNKSMHQMLLHQAVEAQLKSFQLHYQNEGLDSA *F MHRLLAQQQQHQEQQQQHQQQPHHSLGKSQSPAIPS *F GSAGGSSRKSGRFRANWLYQFEWLQYDERANTMFCRHCRKWS *F GELADIRTSFVEGNSNFRLEIVNHHNKCKSHRMCYERE *F LQEQQQHPMPSGSAGGSSKRRSPEIITINVGKNSA *F sincerely *F Terry *F Dr Terence Davis *F Department of Pathology *F University of Wales College of Medicine *F Heath Park *F Cardiff CF144XN *F Wales *F phone \+44-29-20742134 *F fax \+44-29-20744276 *F e.mail davist2@cardiff.ac.uk # *U FBrf0129237 *a Whitfield *b E. *t 2000.7.19 *T personal communication to FlyBase *u FlyBase error report for CG2345 on Wed Jul 19 04:26:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Jul 19 12:20:33 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 19 Jul 2000 12:20:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 19 Jul 2000 04:26:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2345 on Wed Jul 19 04:26:32 2000 *F Content-Length: 1022 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2345 *F Gene annotation error *F Gene CG2345 has incorrect exon/intron structure. *F Comments: Celera sequence of Edg84A (AE003674; AAF54097) has an incorrect *F splice site at *F the 5' junction of the first intron. *F please amend the CDS feature: *F FT CDS join(complement(93721..93732),complement(93105..93665)) *F > *F FT CDS join(complement(93721..93732),complement(93105..93659)) *F and the mRNA feature: *F FT mRNA join(complement(93721..>93732),complement(<93105..93665)) *F > *F FT mRNA join(complement(93721..>93732),complement(<93105..93659)) *F This change means the splice site conforms to the consensus and also translates *F an identical protein to that from AE001574; AAD19810, Celniker et al submission *F of the complete Antp region genomic sequence. A result you would hope for as *F the strains sequenced were the same! *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129238 *a Bilder *b D. *t 2000.6.26 *T personal communication to FlyBase *u FlyBase error report for CG10255 on Mon Jun 26 13:17:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Jun 26 21:12:48 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 26 Jun 2000 21:12:48 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 26 Jun 2000 13:17:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: bilder@rascal.med.harvard.edu *F Subject: FlyBase error report for CG10255 on Mon Jun 26 13:17:39 2000 *F Content-Length: 332 *F Error report from David Bilder (bilder@rascal.med.harvard.edu) *F Gene or accession: CG10255 *F Missed gene *F Comments: This gene has been named dLAP1 (see Bilder et al. 'Collective *F nomenclature for LAP proteins' Nature Cell Biology (2000)vol.2(7)) *F Browser: Mozilla/4.04 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129239 *a Whitfield *b E. *t 2000.7.20 *T personal communication to FlyBase *u FlyBase error report for CG18640 on Thu Jul 20 03:22:21 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jul 20 11:16:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 20 Jul 2000 11:16:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 20 Jul 2000 03:22:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18640 on Thu Jul 20 03:22:21 2000 *F Content-Length: 654 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18640 *F Gene annotation error *F Gene CG18640 has incorrect exon/intron structure. *F Comments: CDS and mRNA features of Amy-p (AE003804; AAF57896) are annotated as *F fragments. *F By simply expanding both features to include the next 3 bases you translate the *F stop codon \- and then you have full length translation! *F FT mRNA 135574..>137071 *F > *F FT mRNA 135574..137074 *F FT CDS 135590..>137071 *F > *F FT CDS 135590..137074 *F it is as easy as that! *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129240 *a Sun *b H. *t 2000.7.10 *T personal communication to FlyBase *u FlyBase error report for CG17117 on Mon Jul 10 14:29:21 2000. *F From mbyhsun@ccvax.sinica.edu.tw Mon Jul 10 23:24:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 10 Jul 2000 23:24:38 \+0100 *F Date: Mon, 10 Jul 2000 14:29:21 \+0800 *F From: Henry Sun *F X-Mailer: Mozilla 4.7 en (Win98; I) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F Subject: annotation correction *F Content-Type: multipart/mixed; boundary='------------E118642991DC502B5DFEC381' *F Content-Length: 807973 *F Dear Flybase: *F I have a revised annotation of the homothorax locus (CG17117), based on *F comparison of the Celera/BDGP genomic seq and the following cDNA *F sequences: *F AF032865 (Kurant et al.; Salzberg in the attached figure) *F AF026788 (Rieckhof et al.; Mann in the figure) *F AF036584 (Pai et al.; \#5 in the figure) *F AF035825 (Pai et al., \#7 in the figure) *F \#4 cDNA (Sun, unpublished) *F The transcript should be 117,801 bp, encoding two protein isoform of 487 *F or 472 aa. The genes has 14 exons. One alternatively spliced exon is *F exon 8 and exon 8'. Exon 8' is only 3 bp. Both protein isoforms are *F represented by multiple cDNA clones. Several relevant files are *F attached. The two figures are in Canvas 6 and the two text files are in *F Word98. *F Henry Sun *F Institute of Molecular Biology *F Academia Sinica *F Nankang, Taipei 11529 *F Taiwan, Republic of china *F [FlyBase curator comment: attached figures and word files are archived.] # *U FBrf0129241 *a Pardue *b M.L. *t 2000.7.15 *T personal communication to FlyBase *u FlyBase error report on Sat Jul 15 21:18:20 2000. *F From bdgp-owner@fruitfly.bdgp.berkeley.edu Sat Jul 15 21:18:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 15 Jul 2000 21:18:20 \+0100 *F Mime-Version: 1.0 *F Date: Sat, 15 Jul 2000 16:27:38 \-0400 *F To: bdgp@fruitfly.bdgp.berkeley.edu *F From: Mary Lou Pardue *F Subject: bdgp correction for sequence in celera sequence AE003734 *F Sender: owner-bdgp@fruitfly.bdgp.berkeley.edu *F In looking through the recent release of sequence I realized that *F there is an error in the sequence of the 93D heat shock gene, Hsr-omega. I *F assume it is the result of screening to avoid repetition of sequences--but *F in this case the screening has deleted repeats that belong there. The *F Hsr-omega gene has a nuclear transcript that includes a well-conserved set *F of 284 bp repeats, flannked on either end by several fragments of the *F repeat. These repeats are transcribed and form part of a nucleus-limited *F transcript of unknown function. We have measured the numbers of 284 bp *F repeats and their variation and find that there are never less than 5 kb *F nor more than 16 kb in any fly (Hogan et al., 1995. Genetics *F 139:1611-1524.) The Celera sequence AE003734 matches well with our 5' end *F of this gene (U18307) from Celera 81973 to 85292 wuth some stuttering *F around 84K-85k. This stuttering is in the region where the gene has some *F partial 284 bp repeats and then begins the long string of good repeats. By *F 86518 the Celera sequence has picked up the non-284 bp repeat region that *F we have sequenced at the 3' end of the Hsr-omega RNA (U02277) Sequences *F for the 284 bp repeats are given in M14556 and M14578-M14583. *F Our studies indicate that the sequence of this region is best given by the *F sequence in U18307, followed by 5-16 kb of 284 bp repeats, followed by the *F sequence in U02277. The number of kb of repeats depends on the fly but *F 5-16 kb forms the limits we have seen in estensive studies of lab stocks. *F \--------------------------------------------------------------------------- *F To (UN)SUBSCRIBE, send e-mail to bdgp-request@fruitfly.bdgp.berkeley.edu *F with '(un)subscribe' as the first line in the BODY of the message. *F \--------------------------------------------------------------------------- # *U FBrf0129242 *a Myat *b A. *t 2000.7.21 *T personal communication to FlyBase *u FlyBase error report for CG7555 on Wed Jun 21 17:35:16 2000. *F From anna.myat@kcl.ac.uk Wed Jun 21 17:28:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 21 Jun 2000 17:28:18 \+0100 *F X-Sender: stty4970@mail.kcl.ac.uk *F Mime-Version: 1.0 *F Date: Wed, 21 Jun 2000 17:35:16 \+0100 *F To: flybase-help@morgan.harvard.edu *F From: Anna Myat *F Subject: CG7555 *F We have submitted a sequence to the EMBL database that corresponds to *F CG7555. Our accession number is: *F Accession#: AJ278468 *F Status: CONFIDENTIAL UNTIL 15-JUN-2001 *F Description: Drosophila melanogaster mRNA for putative ubiquitin ligase *F (DNedd4 gene) *F We have called this gene DNedd4 due to its homology with vertebrate Nedd4. *F We have submitted a paper entitled 'DNedd4, a ubiquitin ligase, is required *F for Commissureless to regulate levels of the Roundabout receptor during *F axon guidance.' Authors: Anna Myat, Veronica McCabe & Guy Tear. We will *F make the information available as soon as it is in press. *F Let me know if you require any further information *F Anna Myat *F Dr. Anna Myat *F MRC Centre for Developmental Neurobiology, *F 4th floor (North), *F New Hunts House, *F King's College London, *F Guy's Campus, Tel: 020 7848 6532 (office) *F London SE1 1UL 6554 (lab) *F U.K Fax: 020 7848 6550 # *U FBrf0129243 *a Whitfield *b E. *t 2000.7.20 *T personal communication to FlyBase *u FlyBase error report for CG17876 on Thu Jul 20 04:03:07 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jul 20 11:57:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 20 Jul 2000 11:57:07 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 20 Jul 2000 04:03:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17876 on Thu Jul 20 04:03:07 2000 *F Content-Length: 580 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17876 *F Gene annotation error *F Gene CG17876 has incorrect exon/intron structure. *F Comments: Amy-d translation (AE003804; AAF57894) stops just short of the stop *F codon. *F please update the CDS and mRNA feature: *F FT mRNA complement(<129579..>131060) *F > *F FT mRNA complement(129576..>131060) *F FT CDS complement(<129579..131060) *F > *F FT CDS complement(129576..131060) *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129244 *a Mueller *b J. *t 2000.7.21 *T personal communication to FlyBase *u FlyBase error report for CG1262 on Thu Jul 20 18:45:51 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jul 21 02:39:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 21 Jul 2000 02:39:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 20 Jul 2000 18:45:51 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jlm62@cornell.edu *F Subject: FlyBase error report for CG1262 on Thu Jul 20 18:45:51 2000 *F Content-Length: 497 *F Error report from Jacob Mueller (jlm62@cornell.edu) *F Gene or accession: CG1262 *F cDNA or EST error *F Comments: The prediction of CG1262 has an approx. 6.5 kb intron. I am a grad *F student in the Wolfner Lab that has done work on 62F *F and RT-PCR and cDNA sequences suggest that no such intron exists. see mRNA *F sequence U85763 and Wolfner et al. Insect Biochem. Molec. Biol. 27(10):825--834. *F thanks, jacob *F Browser: Mozilla/4.08 en (Win98; U ;Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129245 *a Whitfield *b E. *t 2000.7.21 *T personal communication to FlyBase *u FlyBase error report for CG10079 on Fri Jul 21 03:07:08 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jul 21 11:01:19 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 21 Jul 2000 11:01:19 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 21 Jul 2000 03:07:08 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10079 on Fri Jul 21 03:07:08 2000 *F Content-Length: 570 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10079 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG10079. *F Comments: Egfr is known to have 3 alternatively splice isoforms, as discussed *F by Schejter *F et al, Cell 46:1091-1101(1986): *F K03054; AAA51462 *F K03417; AAA51460 *F K03418; AAA51461 *F Celera has annotated only isoform type I (AE003454; AAF46732). *F could you please add CDS features for the remaining 2 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129246 *a Godt *b D. *t 2000.7.22 *T personal communication to FlyBase *u FlyBase error report for CG6977 on Fri Jul 21 20:47:21 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Jul 22 04:41:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 22 Jul 2000 04:41:26 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 21 Jul 2000 20:47:21 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: dgodt@zoo.utoronto.ca *F Subject: FlyBase error report for CG6977 on Fri Jul 21 20:47:21 2000 *F Content-Length: 14924 *F Error report from GODT, DOROTHEA (dgodt@zoo.utoronto.ca) *F Gene or accession: CG6977 *F Missed gene *F Comments: I obtained the complete sequence of EST-SD071473, which I would like *F to report. This EST is not a full-length cDNA clone but so far seems to be the *F largest EST available for this cadherin encoding gene. The EST-sequence *F together with an analysis of the putative intron-exon structure, and the *F putative protein sequences indicate that the previously predicted gene/protein *F structure is incorrect/incomplete. *F new CG6977 gene annotation: *F 1. EST SD071473 sequence *F GGTGGCCGAAGAGACAGACACCAATCCACGGCTATCCTCCACGGCAACGATTACCGTTAGTGTTTTGGATGCCAACGACA *F ATAAGCCAGTTTTCGAGCAGGAGAGCTATTCTGCATCTGTTTCGGAGGCGGCGCTTCCGGGTCAGTATATCGCCACCATT *F ACGGCTCGGGATGTGGACTCCGGAAGTTATGGTGACTCTGGCATTCGGTACAGCTTATCCGGAACCGGTGCTGAACTTTT *F CCATGTCAATGAGCAGACAGGTGTTATAAGCCTAGCCAATTGCCATGACAATGGGGAGAGCAATAGACGCGAGCGACGTG *F ATCTGAACGAGGATGAGCATGTCGAGGAGGACGATGGCGAGGGTCACCTGGAAATGCTCTCCATGGAGGCAGCAACTCGA *F GAAATTGGCACAGAGCCCACCGTCCAGTACACGTTAATCACCCAGGCGCCAGAGGAGCAGGCATCGTCGGTTCCACTACC *F AGCTCCTGTTCCACACGCAGCTCCATCTGGAGTTCCCGCAGCGACTGCTAATGACGACAAGGCACCGCAGACGTGTCTGG *F ACTACGAATCGGAGACCACGTACTTCTTGTCATACAAGGCCACCGACGATAACGGACGTGGATCCGCATCTGTAGTATCC *F CTACGGATTTCTGTAACTGATGCCAACGATTCCCCGCCTGTCTGCGAAAGTCCCCTGTATAGGGCGAGCGTGGATGAGGG *F AGCCGTGGTCTTTGATTCCCCGCTGATAGTCAAGGCAAGGGATGCGGACACCATGTCCAGAATTAGCTACAGAATCCGGG *F GCTCAGAGCAGGTCGAGAGCATTTTCGATATTGATCGTGAGACCGGGCAGATCATCATCAGGCCGAATGCCACACTAGAT *F GTGACCAATTTAAATAGCGATCAACTTATCTTCGCTGTGGAAGCCAACGATGGACTATTTACTGCTCACTGCGGCGTAAA *F CATCACCGTTCGGGATGTAAATAACCACGTACCCAATTTCGAGCAGCAGAGCTACAGTGCCGTCGTCGAGGAGAACTCGG *F AGATTGGAACGAGCGTGGAGCGGGTGCATGCAACCGACTTGGATACGGGCAAGAATGCCGAGCTACGCTACCGCATACAA *F CAGGGCAGCTTTGACGATTTTGGCATTGTTGAGACCACCGGCGAGGTGTTTGTTTCTCGAAAACTGGACTTCGATCGACG *F CAACACCTATCAGCTGCAGATTCAGGCTTCCGATCAGGGAACCCCAAGTCTAACGGGAACCGCCACCCTGACGATAAATG *F TTCAGAACAGTAATGATAAGGATCCGTATTTTGTGCCAGCTACACAGCATGCTGAGGTGCGTGCAGACGCTCCTCCCGGG *F CAATTGGTCTACACTCTAATCGCCCTGGATCCCGATGTGGCCAACCATAATGCTTTGGAATTTGCCGGCACCGACGACAT *F CACCGCAATCGATAAGGAAGGCAAGGAGTTGCCGCATTATGATCAGTTCAAGGAGTACTTTAAGATTTCCAGAAACGGAA *F AGGTTTCGGTTAACAAGCAGTTGGATCGCAATTTGTTTGCTGTGATGAGGATCAACGTTCTGGTTACGGACAGCACAGCT *F CCCAATGTTCAGCAGGGTCGTGGTTTGCTCATCATACAGATCATTGATGTCAACAAGAATCCACCGCGTTTCAATGCACC *F ATGGAGTGTGGAGCAGCCGCAAATCAAGCTGCAAATGGTGGAGGAGCAGCCTGTGGGCACCGTACTGACCACTCTTCAGG *F CCAACGATGAGGACTCCAGCATTGGCGAGTTTAATATCAGTGACAACGACTATTTCGCCATTAACCAGACCAGCGGAATG *F ATCTACACCATTGCCCGACTCGACTACGAAGTCGTAAAGGAGGTCAAGTTCCAAGTCACCGTCAGTGATACGGGTGTGCC *F TGCTTTAACAGCCACCGCTGATGTGGTTGTGGACATTATTAACCTGAACGATAATGACCCAAAGTTCTCTCAATCTGATT *F ATTACTTCAATGTTACGGAAAACTCACCCCGCGGCACGGTGGCGGGAAAAGTTGAGGCCCACGATGGCGATGTGGGCGTC *F TTTGGCGAAATCACTTACACGCTGATCGGCGAAAATAACAAGTACTTTAGCATTGATGCCTATACGGGCAACGTAATGGT *F GGCCAATTCCTCGATTCTCGATCGAGAGCAAATTAAGGAGCTCACGCTCTCCGTGGTGGCTCAGGATAAGGCTCCAGCTG *F CAGTCCAAAAATCGGCTACGGCAACGATCCACATAAACATTTTGGATGTCAATGATAATGCTCCCGTTTTTACACGAGAT *F GTATACAACTCTACGGTGGCGGAAAATGCCGCCTATCAGCCGCCTGCAGCATTGCTCCAAGTTCAGGCCATTGACCAGGA *F TGAGGGTCTATATGGAGATGTGCGGTATATCATTACAGCCGGCAATGAAATGGGACTCTTCAAGCTCGATGCGCAGTCTG *F GCATTGTGTACCCAGCGCAAAGTTTGTCCGGAAAACATGGAGCCTACGAGCTAACCATTTCGGCACGCGACACTCAAGGA *F TCGGGCACCATGGAAAGCACAACGAAAGCGATTATAACTGTACTAAGGGTTAACCGCCATAAGCCGGAGTTCGTCATACC *F CGCGCTGTCCAATGCCACCATTGAGATACCTGGTGATATTGTCCAGCCCGATTATCTACTGCTAACCGTCAGGGCAATGG *F ATAATGATACGGAGGAAAACGGCAAAGTTAGTTACCACCTGCAGGTGAACAATCGGAACGAGCAGCAGACCGGGGAATTC *F AAGATCGATGAAGTGACAGGAGAATTGCGAGCCAAGACGCAGCTAAACCGTAAAAACCGCGCCAACTACGATATAATACT *F AGTTGCCCGAGATGCAGGCAATCCTCCATTTGAATCCCTGCGTCTCCTCAGCGTCAGCATTGTGGATGCCAACGAGAACC *F GGCCGGAGTTTCCCGATGCCTCCAATCCTTATAAGGTGTCGATCAATGAGAATAGCGGTCGGGATGTGAAGATTGGACAC *F ATTCAAGCGGCCTCAAGAAGCAAGCACAACCGGGATATATTCTATTACATGCTGTTGGGCAATGAGGATGGTGCGTTTTA *F TGTGGACAAACTGACTGGGGATATTTACACAAACAAGAGCCTAGATCGCGAAGAAACGGATGTGTACACTTTATACATCC *F TAGCCAGCATCAAGGCGGATCTGCATATATCCGAAGAGGAACGCGCCTCATTCTCGATTAAGACCCTCAACCGGGATAAC *F ACAGTCGCAAAAGTTGCCATCACTGTCTTGGATGTTAACGACAATCCTCCCGTGTTTGAAAAGCCCATTTACTATGCCGG *F AGTAAACGCCAATGCCAAGATGGGCGCAGCTATTACGCTAGTAAATGCAACGGATGCAGATCAAGGCAAGAACGCAAAGA *F TCGAGTTTATGATCGTCGCGTCGAATCTGTATAAGTTTGGAGCCACCAAATCCACAGGATCGATCGTTCCCAGTCCGTTT *F GCCATTTCGCAGGATGGTCGCATCTCAGCAAACACTATCATGGCTGAGTACAACCAGGATCGTTTCGAACTGGAGATCGT *F GGCAAGAGAATTGGAGCAACCCCAGAGCTCAGCCAGTACTAAAGTGAATATCTGGGTCTTTGATGGCACGCAGCTGGTGC *F GCGTAATTTTGTCCCGTCCGCCGGAAGAGGTTTACCAGGAACAGGAGGAGATCATTGCTGAGTTGCGCAATGCCACCCAG *F CATCGCATCATCGTTGATGAAATAAGATTCCATTTGGACTCGATTGGACGCATACGTATGGACTGGTGTGACCTGTACTT *F CCATGCAGTTGATCCTCAGACCCAGCAAATTGCACCAGTTGATGAGATACTCAAGGATATCGACAGGAACTACGATTATC *F TAAAGGACTACTACGCTGGTTTTGCCATAGAGAACGTTGTGCCCGCCTACATAGCTATTGTGCAAGATGAGTTTGATCTA *F GCAGTGGCTGGATTGGTGGCGCTGGTCATCGTCCTTTTCGTTGGCGTTATTAGCTTTATTGTCCTGTGCTGCTGCCTAAA *F GCATTGGAATCTGTCGGTGCCCGTGGAGACTCGTCGCAAGGAGGCCCTAATTAAGAAACAGATTATAGAGGACCTAAACA *F CTACCGAAAATCCGCTGTGGATAGAACAAAAATTGAAACTGTATGAGGAACAGGAACTGACAATGCAGGTTTTCTCGGAG *F CCCGATCACATATCCAACTCTGAGGCACCGGGCCACCTCGACCATCGTAGCTCTCTTGAGCAGGTTCATCATGTGGGCCA *F GACGGTGGACAACACGTATGCCACCATTCAGCCGCGCAATAATCAAAATCGTCTTACTGGCGGCGGTGGTGCCGGCGGTG *F GGTCGATGCGAAGCGGGGGAGGTGCCAGCGCCGGAGGAGTGGGAGGTGCTGGTCTACTCCTGGCCCGCGTTGATCCGCAC *F ATGAATGAATTTGCGGATTATGCCACGTTGCGAAACAATAGAGCGCCATCGTTGTACGAGTTCACAGGATCCACATTCCA *F GGCTCCCATCCGGGACGGAGACGACGCCGTGGCCGAGCTGATCTAAGGGTCGATGGGAACTGTTGCCCAAAAAGATGTGT *F AAATTATGTAGTAGCTGTGTATCTGTGTTGTAGACAAGCCCTCGCCGTGGTTAAGTTCAACCGAATCTGTTCTGTTGCAC *F CTTTGAGTTCGTCGTTGCGTTTTCATCAACAATATTCTAGACACGTACCGTACATAGCACATATAAATACTTATATACAT *F AGCGTAGTTGTAACTGGTAAGCCAGCGTTTATCTAGAACTCAAGTAATCAGGATAGAGAGCCAGGAGCAGAAACAGAACT *F ACAAGAACCCATAGAGGAAATTCTCCCGCATAATTAATGACAATAATATATATGTACTATTATATATTGACAATGTCTAG *F CCGTAATGAAACGATATGAATATGTAAGAGTTACTAATGTACATCTACAATGGCATCTACACAATGCAGTCTTTACTCGA *F AAACTTCCGCGATATTAGTTTTAATGGCGATCCAAATCGTCTACAGCTTAAGTTGTACCACTAAAAAGACTAAATTATTT *F GCTCTTCCAATTCCGTAACACGTTTAGTTGTGTGTTAACAATTAGTTACTTAGATTCTAGTTTAATTAGCCAAACTTGCT *F CATACTTATAAGTTCAGAATTTAAGTTGGAGTGAATTGGAAATGTGCAACGAACTAAGTTACTTTTGACCGCTGTCCGAC *F TCCTCACGGGCAGAGCCAGAAACTCATTTATGTTTGCATTTACATTAAACATATACACATACAAAAAAAAA *F 2. predicted transcript (ATG \--- polyA) *F ATGAAGCTCCCCTTGGGGCTGTTGATGATCTGTTTGGGCTTGACCCTCGCTAAAGGAGAGACAAATCTGCCACCTGTATT *F CACGCAGACCCTGAACAATATTATTCTCTACGAGAATGTAACTGTGGGTACGGTAGTTTTTCGTCTAGAAGCGTACGATC *F CCGAAGGAAGTCCTGTTACCTATGGAGCCATCGGTGCGGATCACTTTAGCGTGGATCCGGTTTCGGGAAACATAACGCTG *F ATAAAACCACTAGATCGCGAGGAGAAGGACACCTTGAAGTTCCTGGTGTCGATAAGGGATCGCGTAGATCCCGAGGGAGA *F GTCGGAAAGGGATAATGTGGTCGAAGTGCCGATTACATTTATTATTCTTGATCTGAACGACAATCCACCAGAATTTCAAA *F ATACTCCCTACGAAGCGGATGTAAACGAGGATGCTGCGGTGGGAACAACTATATTTGATAAGATTACAGTCAAGGATAGG *F GATATAGTTGGCGAAAGTCTCGACTTGAAATGCTTGCCGCAACAGCAAAGTCCCGAAGCTTGCAGAAAATTCCGCTTGCA *F TATTATAAAGCGAGATGCCACAATTCTGGAAGCAGCAGTTGTGCTCAATGATACCCTGAACTACAATCAGCGGATGGTTT *F ACCACTTCCAAATCGAAGCCACCGATGGCCCGCACAAGACGCAAACAACGTTTGAGGCGAGGGTGAAGGATGTTCAGGAT *F AAGCCGCCCGTATTTCAGGGCTCCCTATCTACGGTTATTGATGAGGACAGTCCCATTAACACCTTGGTGCTTACTGTCCA *F CGCTAGGGATGGCGATACGGGCGAACCAAGGAAAATTGTCTATGATCTGCGGACAAATCCCAATGATTACTTCCTGCTGG *F ATGCTCAGACAGGAGAACTGCGTACAGCTAAACCATTAGATCGGGAAGCTTTGGAAGATTCCACTGGTATTATCTCACTG *F GTGATTCGCGCTCGTGAGCTTGTGAACGGAGTGCCCAGCGATGATCCCCTGACAAGTGCTACGGCCAAGGCAACGGTGAC *F CATTAGGGATGTAAACGACTCTCCACCGGTTTTTAATCACAAGGAGTACTCCGTGTCCCTTTTGGAGAACACTCTACCTG *F GCACACCGCTCGCACTGGACATGAGCGTCAGTGATGCTGACGTGGGCATTAATTCCAAATTTGCCCTGCGCCTGGATGAC *F GTTTCCGGTGTATTCGATGTTGAACCTAAGTTGGTCACCGGGTACTCGCAGGTCAATATTCGCGTCGCAAACGGCACTCT *F AGACTACGAGAATCCAAACCAGCGAAAGTTCATCGTTCTGGTGGTGGCCGAAGAGACAGACACCAATCCACGGCTATCCT *F CCACGGCAACGATTACCGTTAGTGTTTTGGATGCCAACGACAATAAGCCAGTTTTCGAGCAGGAGAGCTATTCTGCATCT *F GTTTCGGAGGCGGCGCTTCCGGGTCAGTATATCGCCACCATTACGGCTCGGGATGTGGACTCCGGAAGTTATGGTGACTC *F TGGCATTCGGTACAGCTTATCCGGAACCGGTGCTGAACTTTTCCATGTCAATGAGCAGACAGGTGTTATAAGCCTAGCCA *F ATTGCCATGACAATGGGGAGAGCAATAGACGCGAGCGACGTGATCTGAACGAGGATGAGCATGTCGAGGAGGACGATGGC *F GAGGGTCACCTGGAAATGCTCTCCATGGAGGCAGCAACTCGAGAAATTGGCACAGAGCCCACCGTCCAGTACACGTTAAT *F CACCCAGGCGCCAGAGGAGCAGGCATCGTCGGTTCCACTACCAGCTCCTGTTCCACACGCAGCTCCATCTGGAGTTCCCG *F CAGCGACTGCTAATGACGACAAGGCACCGCAGACGTGTCTGGACTACGAATCGGAGACCACGTACTTCTTGTCATACAAG *F GCCACCGACGATAACGGACGTGGATCCGCATCTGTAGTATCCCTACGGATTTCTGTAACTGATGCCAACGATTCCCCGCC *F TGTCTGCGAAAGTCCCCTGTATAGGGCGAGCGTGGATGAGGGAGCCGTGGTCTTTGATTCCCCGCTGATAGTCAAGGCAA *F GGGATGCGGACACCATGTCCAGAATTAGCTACAGAATCCGGGGCTCAGAGCAGGTCGAGAGCATTTTCGATATTGATCGT *F GAGACCGGGCAGATCATCATCAGGCCGAATGCCACACTAGATGTGACCAATTTAAATAGCGATCAACTTATCTTCGCTGT *F GGAAGCCAACGATGGACTATTTACTGCTCACTGCGGCGTAAACATCACCGTTCGGGATGTAAATAACCACGTACCCAATT *F TCGAGCAGCAGAGCTACAGTGCCGTCGTCGAGGAGAACTCGGAGATTGGAACGAGCGTGGAGCGGGTGCATGCAACCGAC *F TTGGATACGGGCAAGAATGCCGAGCTACGCTACCGCATACAACAGGGCAGCTTTGACGATTTTGGCATTGTTGAGACCAC *F CGGCGAGGTGTTTGTTTCTCGAAAACTGGACTTCGATCGACGCAACACCTATCAGCTGCAGATTCAGGCTTCCGATCAGG *F GAACCCCAAGTCTAACGGGAACCGCCACCCTGACGATAAATGTTCAGAACAGTAATGATAAGGATCCGTATTTTGTGCCA *F GCTACACAGCATGCTGAGGTGCGTGCAGACGCTCCTCCCGGGCAATTGGTCTACACTCTAATCGCCCTGGATCCCGATGT *F GGCCAACCATAATGCTTTGGAATTTGCCGGCACCGACGACATCACCGCAATCGATAAGGAAGGCAAGGAGTTGCCGCATT *F ATGATCAGTTCAAGGAGTACTTTAAGATTTCCAGAAACGGAAAGGTTTCGGTTAACAAGCAGTTGGATCGCAATTTGTTT *F GCTGTGATGAGGATCAACGTTCTGGTTACGGACAGCACAGCTCCCAATGTTCAGCAGGGTCGTGGTTTGCTCATCATACA *F GATCATTGATGTCAACAAGAATCCACCGCGTTTCAATGCACCATGGAGTGTGGAGCAGCCGCAAATCAAGCTGCAAATGG *F TGGAGGAGCAGCCTGTGGGCACCGTACTGACCACTCTTCAGGCCAACGATGAGGACTCCAGCATTGGCGAGTTTAATATC *F AGTGACAACGACTATTTCGCCATTAACCAGACCAGCGGAATGATCTACACCATTGCCCGACTCGACTACGAAGTCGTAAA *F GGAGGTCAAGTTCCAAGTCACCGTCAGTGATACGGGTGTGCCTGCTTTAACAGCCACCGCTGATGTGGTTGTGGACATTA *F TTAACCTGAACGATAATGACCCAAAGTTCTCTCAATCTGATTATTACTTCAATGTTACGGAAAACTCACCCCGCGGCACG *F GTGGCGGGAAAAGTTGAGGCCCACGATGGCGATGTGGGCGTCTTTGGCGAAATCACTTACACGCTGATCGGCGAAAATAA *F CAAGTACTTTAGCATTGATGCCTATACGGGCAACGTAATGGTGGCCAATTCCTCGATTCTCGATCGAGAGCAAATTAAGG *F AGCTCACGCTCTCCGTGGTGGCTCAGGATAAGGCTCCAGCTGCAGTCCAAAAATCGGCTACGGCAACGATCCACATAAAC *F ATTTTGGATGTCAATGATAATGCTCCCGTTTTTACACGAGATGTATACAACTCTACGGTGGCGGAAAATGCCGCCTATCA *F GCCGCCTGCAGCATTGCTCCAAGTTCAGGCCATTGACCAGGATGAGGGTCTATATGGAGATGTGCGGTATATCATTACAG *F CCGGCAATGAAATGGGACTCTTCAAGCTCGATGCGCAGTCTGGCATTGTGTACCCAGCGCAAAGTTTGTCCGGAAAACAT *F GGAGCCTACGAGCTAACCATTTCGGCACGCGACACTCAAGGATCGGGCACCATGGAAAGCACAACGAAAGCGATTATAAC *F TGTACTAAGGGTTAACCGCCATAAGCCGGAGTTCGTCATACCCGCGCTGTCCAATGCCACCATTGAGATACCTGGTGATA *F TTGTCCAGCCCGATTATCTACTGCTAACCGTCAGGGCAATGGATAATGATACGGAGGAAAACGGCAAAGTTAGTTACCAC *F CTGCAGGTGAACAATCGGAACGAGCAGCAGACCGGGGAATTCAAGATCGATGAAGTGACAGGAGAATTGCGAGCCAAGAC *F GCAGCTAAACCGTAAAAACCGCGCCAACTACGATATAATACTAGTTGCCCGAGATGCAGGCAATCCTCCATTTGAATCCC *F TGCGTCTCCTCAGCGTCAGCATTGTGGATGCCAACGAGAACCGGCCGGAGTTTCCCGATGCCTCCAATCCTTATAAGGTG *F TCGATCAATGAGAATAGCGGTCGGGATGTGAAGATTGGACACATTCAAGCGGCCTCAAGAAGCAAGCACAACCGGGATAT *F ATTCTATTACATGCTGTTGGGCAATGAGGATGGTGCGTTTTATGTGGACAAACTGACTGGGGATATTTACACAAACAAGA *F GCCTAGATCGCGAAGAAACGGATGTGTACACTTTATACATCCTAGCCAGCATCAAGGCGGATCTGCATATATCCGAAGAG *F GAACGCGCCTCATTCTCGATTAAGACCCTCAACCGGGATAACACAGTCGCAAAAGTTGCCATCACTGTCTTGGATGTTAA *F CGACAATCCTCCCGTGTTTGAAAAGCCCATTTACTATGCCGGAGTAAACGCCAATGCCAAGATGGGCGCAGCTATTACGC *F TAGTAAATGCAACGGATGCAGATCAAGGCAAGAACGCAAAGATCGAGTTTATGATCGTCGCGTCGAATCTGTATAAGTTT *F GGAGCCACCAAATCCACAGGATCGATCGTTCCCAGTCCGTTTGCCATTTCGCAGGATGGTCGCATCTCAGCAAACACTAT *F CATGGCTGAGTACAACCAGGATCGTTTCGAACTGGAGATCGTGGCAAGAGAATTGGAGCAACCCCAGAGCTCAGCCAGTA *F CTAAAGTGAATATCTGGGTCTTTGATGGCACGCAGCTGGTGCGCGTAATTTTGTCCCGTCCGCCGGAAGAGGTTTACCAG *F GAACAGGAGGAGATCATTGCTGAGTTGCGCAATGCCACCCAGCATCGCATCATCGTTGATGAAATAAGATTCCATTTGGA *F CTCGATTGGACGCATACGTATGGACTGGTGTGACCTGTACTTCCATGCAGTTGATCCTCAGACCCAGCAAATTGCACCAG *F TTGATGAGATACTCAAGGATATCGACAGGAACTACGATTATCTAAAGGACTACTACGCTGGTTTTGCCATAGAGAACGTT *F GTGCCCGCCTACATAGCTATTGTGCAAGATGAGTTTGATCTAGCAGTGGCTGGATTGGTGGCGCTGGTCATCGTCCTTTT *F CGTTGGCGTTATTAGCTTTATTGTCCTGTGCTGCTGCCTAAAGCATTGGAATCTGTCGGTGCCCGTGGAGACTCGTCGCA *F AGGAGGCCCTAATTAAGAAACAGATTATAGAGGACCTAAACACTACCGAAAATCCGCTGTGGATAGAACAAAAATTGAAA *F CTGTATGAGGAACAGGAACTGACAATGCAGGTTTTCTCGGAGCCCGATCACATATCCAACTCTGAGGCACCGGGCCACCT *F CGACCATCGTAGCTCTCTTGAGCAGGTTCATCATGTGGGCCAGACGGTGGACAACACGTATGCCACCATTCAGCCGCGCA *F ATAATCAAAATCGTCTTACTGGCGGCGGTGGTGCCGGCGGTGGGTCGATGCGAAGCGGGGGAGGTGCCAGCGCCGGAGGA *F GTGGGAGGTGCTGGTCTACTCCTGGCCCGCGTTGATCCGCACATGAATGAATTTGCGGATTATGCCACGTTGCGAAACAA *F TAGAGCGCCATCGTTGTACGAGTTCACAGGATCCACATTCCAGGCTCCCATCCGGGACGGAGACGACGCCGTGGCCGAGC *F TGATCTAAGGGTCGATGGGAACTGTTGCCCAAAAAGATGTGTAAATTATGTAGTAGCTGTGTATCTGTGTTGTAGACAAG *F CCCTCGCCGTGGTTAAGTTCAACCGAATCTGTTCTGTTGCACCTTTGAGTTCGTCGTTGCGTTTTCATCAACAATATTCT *F AGACACGTACCGTACATAGCACATATAAATACTTATATACATAGCGTAGTTGTAACTGGTAAGCCAGCGTTTATCTAGAA *F CTCAAGTAATCAGGATAGAGAGCCAGGAGCAGAAACAGAACTACAAGAACCCATAGAGGAAATTCTCCCGCATAATTAAT *F GACAATAATATATATGTACTATTATATATTGACAATGTCTAGCCGTAATGAAACGATATGAATATGTAAGAGTTACTAAT *F GTACATCTACAATGGCATCTACACAATGCAGTCTTTACTCGAAAACTTCCGCGATATTAGTTTTAATGGCGATCCAAATC *F GTCTACAGCTTAAGTTGTACCACTAAAAAGACTAAATTATTTGCTCTTCCAATTCCGTAACACGTTTAGTTGTGTGTTAA *F CAATTAGTTACTTAGATTCTAGTTTAATTAGCCAAACTTGCTCATACTTATAAGTTCAGAATTTAAGTTGGAGTGAATTG *F GAAATGTGCAACGAACTAAGTTACTTTTGACCGCTGTCCGACTCCTCACGGGCAGAGCCAGAAACTCATTTATGTTTGCA *F TTTACATTAAACATATACACATACAAAAAAAA *F 3. predicted cadherin protein *F RLICLLFADPKMKLPLGLLMICLGLTLAKGETNLPPVFTQTLNNIILYENVTVGTVVFRLEAYDPEGSPVTYGAIGADHF *F SVDPVSGNITLIKPLDREEKDTLKFLVSIRDRVDPEGESERDNVVEVPITFIILDLNDNPPEFQNTPYEADVNEDAAVGT *F TIFDKITVKDRDIVGESLDLKCLPQQQSPEACRKFRLHIIKRDATILEAAVVLNDTLNYNQRMVYHFQIEATDGPHKTQT *F TFEARVKDVQDKPPVFQGSLSTVIDEDSPINTLVLTVHARDGDTGEPRKIVYDLRTNPNDYFLLDAQTGELRTAKPLDRE *F ALEDSTGIISLVIRARELVNGVPSDDPLTSATAKATVTIRDVNDSPPVFNHKEYSVSLLENTLPGTPLALDMSVSDADVG *F INSKFALRLDDVSGVFDVEPKLVTGYSQVNIRVANGTLDYENPNQRKFIVLVVAEETDTNPRLSSTATITVSVLDANDNK *F PVFEQESYSASVSEAALPGQYIATITARDVDSGSYGDSGIRYSLSGTGAELFHVNEQTGVISLANCHDNGESNRRERRDL *F NEDEHVEEDDGEGHLEMLSMEAATREIGTEPTVQYTLITQAPEEQASSVPLPAPVPHAAPSGVPAATANDDKAPQTCLDY *F ESETTYFLSYKATDDNGRGSASVVSLRISVTDANDSPPVCESPLYRASVDEGAVVFDSPLIVKARDADTMSRISYRIRGS *F EQVESIFDIDRETGQIIIRPNATLDVTNLNSDQLIFAVEANDGLFTAHCGVNITVRDVNNHVPNFEQQSYSAVVEENSEI *F GTSVERVHATDLDTGKNAELRYRIQQGSFDDFGIVETTGEVFVSRKLDFDRRNTYQLQIQASDQGTPSLTGTATLTINVQ *F NSNDKDPYFVPATQHAEVRADAPPGQLVYTLIALDPDVANHNALEFAGTDDITAIDKEGKELPHYDQFKEYFKISRNGKV *F SVNKQLDRNLFAVMRINVLVTDSTAPNVQQGRGLLIIQIIDVNKNPPRFNAPWSVEQPQIKLQMVEEQPVGTVLTTLQAN *F DEDSSIGEFNISDNDYFAINQTSGMIYTIARLDYEVVKEVKFQVTVSDTGVPALTATADVVVDIINLNDNDPKFSQSDYY *F FNVTENSPRGTVAGKVEAHDGDVGVFGEITYTLIGENNKYFSIDAYTGNVMVANSSILDREQIKELTLSVVAQDKAPAAV *F QKSATATIHINILDVNDNAPVFTRDVYNSTVAENAAYQPPAALLQVQAIDQDEGLYGDVRYIITAGNEMGLFKLDAQSGI *F VYPAQSLSGKHGAYELTISARDTQGSGTMESTTKAIITVLRVNRHKPEFVIPALSNATIEIPGDIVQPDYLLLTVRAMDN *F DTEENGKVSYHLQVNNRNEQQTGEFKIDEVTGELRAKTQLNRKNRANYDIILVARDAGNPPFESLRLLSVSIVDANENRP *F EFPDASNPYKVSINENSGRDVKIGHIQAASRSKHNRDIFYYMLLGNEDGAFYVDKLTGDIYTNKSLDREETDVYTLYILA *F SIKADLHISEEERASFSIKTLNRDNTVAKVAITVLDVNDNPPVFEKPIYYAGVNANAKMGAAITLVNATDADQGKNAKIE *F FMIVASNLYKFGATKSTGSIVPSPFAISQDGRISANTIMAEYNQDRFELEIVARELEQPQSSASTKVNIWVFDGTQLVRV *F ILSRPPEEVYQEQEEIIAELRNATQHRIIVDEIRFHLDSIGRIRMDWCDLYFHAVDPQTQQIAPVDEILKDIDRNYDYLK *F DYYAGFAIENVVPAYIAIVQDEFDLAVAGLVALVIVLFVGVISFIVLCCCLKHWNLSVPVETRRKEALIKKQIIEDLNTT *F ENPLWIEQKLKLYEEQELTMQVFSEPDHISNSEAPGHLDHRSSLEQVHHVGQTVDNTYATIQPRNNQNRLTGGGGAGGGS *F MRSGGGASAGGVGGAGLLLARVDPHMNEFADYATLRNNRAPSLYEFTGSTFQAPIRDGDDAVAELIZ *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129247 *a Wodarz *b A. *t 2000.7.26 *T personal communication to FlyBase *u FlyBase error report for CG10261 on Wed Jul 26 03:55:01 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Jul 26 11:48:55 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 26 Jul 2000 11:48:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 26 Jul 2000 03:55:01 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: wodarz@uni-duesseldorf.de *F Subject: FlyBase error report for CG10261 on Wed Jul 26 03:55:01 2000 *F Content-Length: 256 *F Error report from Andreas Wodarz (wodarz@uni-duesseldorf.de) *F Gene or accession: CG10261 *F Gene annotation error *F P element l(2)k06403 hits gene CG10261. *F Comments: *F Browser: Mozilla/4.04 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129248 *a Tellam *b R. *t 2000.7.27 *T personal communication to FlyBase *u FlyBase error report for CG2666 on Wed Jul 26 20:15:25 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Jul 27 04:09:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 27 Jul 2000 04:09:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 26 Jul 2000 20:15:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: Ross.Tellam@tag.csiro.au *F Subject: FlyBase error report for CG2666 on Wed Jul 26 20:15:25 2000 *F Content-Length: 5651 *F Error report from Ross Tellam (Ross.Tellam@tag.csiro.au) *F Gene or accession: CG2666 *F cDNA or EST error *F Comments: I have determined a full length cDNA sequence for the homolog of *F CG2666 from Lucilia cuprina. *F >From this highly homologous sequence it is easy to delineate the exon/intron *F structure *F of the Drosophila sequence (CG2666). The latter has been called DmCS-1 and the *F former LcCS-1. *F You will find that some of your exon annotations at the 5'end of CG2666 are *F incorrect probably *F due to the presence of both small exons and small introns which confound the *F gene prediction programs. *F MSNIRHRPLAPPGTGDSDDNFTDDESTPLTQDIYGGSQRTIQETKGWDVF *F RDPPIKIETGSTANQECLELTVKILKIFAYIFTFIIVLLGGVVAKGCVLF *F MTSQIRKDKRIEYCNKDLGRDKTFVVKLPEEERIAWIWALIIAFSIPEVG *F SLIRSARICFFKTFKVPKTGHFLFMFIMESLNTFGTALLMFVVLPQLDAI *F QGAMLTNCLCVIPGILGLMSRTSKEGKRFAKMLIDLVAVAAQVTGFVIWP *F LLENRKELWVIPVACLMVSCGWWENYVTSQSSFALIRSMGRVKDDLKYTR *F YFSHMFLSIWKISLFLCSVLLIYWIQGEEPGNIFGLFSDAFGPHKISVDE *F LATTLTNSLPDTLDAADIDSIDIEASYNTVVYVLLIQIFGAYLCYIFGKF *F ACKILIQGFSYAFPVSLTVPVAVSLLIAACGIRIDDPCFFHDTIPDYLFF *F TSPSNFRSNDFVTNQMAWAWILWLLSQTWIGLHIWTPKCERLATTEKLFV *F KPMYCSLLIDQSMAMNRRRDDQADVKTEDLSEIEKEKGDEYYETISVHTD *F GSAIQSKPTIKSSDHITRIYSCATMWHVTKDEMIEFLKSIMRMDEDQCAR *F RVAQKYLRIVDPDYYEFETHTFFDDAFEISDHSDDDIQVNRFVKLLVATM *F DDAASEIHQTRIRIRPPKKYPTPYGGRLVWTLPGKTKFFAHLKDKDRIRH *F RKRWSQVMYMYYLLGHRLMELPIPVDRKDTIAENTYLLTLDGDIDFKPNA *F VTLLVDLMKKNKNLGAACGRIHPVGSGPMVWYQLFEYAIGHWLQKATEHM *F IGCVLCSPGCFSLFRGKALMDDNVMKKYTTQSDEARHYVQYDQGEDRWLC *F TLLLQRGYRVEYSAASDAYTHCPEGFNEFYNQRRRWVPSTIANIMDLLGD *F AKRTIKINDNISLLYIFYQMMLMGGTILGPGTIFLMLVGAFVAAFRIDNW *F TSFHYNIIPILGFMFICFTCKANIQLFVAQVLSTAYALIMMAVIVGTALQ *F LGEDGIGSPSAIFLISMVGSSFIAACLHPQEFWCISAGLIYLLSIPSMYL *F LLILYSIINLNAVSWGTREVVAKKTKKEMEAEKKAAEEAAKKAKQKSMLG *F FLQGGVGNNGDEEGSVEFSLAGLSRCIFCTHGKTSDEKQQLTTIAESLDT *F IKNRIDSIEQTINPHDQHSHRHGRRRTTSSGSKDHHLLSSVAEKSGDESE *F ESDTDTSVERKQERDFLTNPYWIEDPDLRKGEVDFLSSAELQFWKDLNDK *F YLFPIDNDPVEQARIAKDLKELRDSSVFFFFMVNALIVSIVFLLQLNKDN *F IHVKWPFGVRTNITYDESTQEVHISKDYLQLEPIGLVFVFFFALILVIQF *F TAMLFHRFGTLSHILASTELNFFKKKSEDLSQDALIDKHAVEIVKNLQRL *F QGIDGDYDNDSGSGPDRIARRKTIQNLEKARQPRRQIGTLDVAFKKRFMK *F LTAEAENNPSTPILTRRLTMRAETIRALEVRKNSVMAERRKSAMQTLGAK *F NEYGIAAVSTLNNNGGIPNVRSGRVSNAGINIKDVFNVNGGPSEQIYGSN *F NGAVNQGYEHVNEDDDGNSLRLTTRNPPQVTWGTYSSNTGRM *F CCATTTGTGTGTAACTAGGCTGATTTGGCCGATGGGGTGTGATCGTGTGT *F TGTGTTTGTGTGTAACCGTGGGATATAGAGAGAGATGTCTAATATAAGAC *F ATCGCCCTCTGGCCCCACCGGGTACGGGCGACAGTGATGACAACTTTACA *F GATGATGAGAGTACACCATTGACACAAGATATTTATGGCGGAAGCCAACG *F TACTATACAGGAAACAAAAGGCTGGGATGTATTCAGAGATCCACCCATTA *F AAATAGAAACAGGCTCAACGGCAAATCAAGAATGTCTAGAATTAACTGTA *F AAAATTTTAAAAATTTTCGCCTATATCTTTACGTTTATAATCGTATTGTT *F GGGAGGTGTGGTGGCAAAAGGCTGTGTATTATTTATGACATCACAAATCC *F GTAAAGATAAGAGAATAGAATACTGTAATAAAGATTTAGGTCGTGATAAA *F ACATTTGTGGTTAAATTACCCGAAGAAGAACGTATAGCCTGGATCTGGGC *F TTTAATTATAGCCTTCTCGATACCCGAAGTGGGTTCTCTTATACGTTCGG *F CTCGTATTTGTTTCTTCAAAACTTTTAAAGTTCCCAAGACGGGTCATTTT *F CTATTTATGTTTATTATGGAATCTTTGAATACCTTTGGTACTGCTCTTTT *F AATGTTTGTTGTTTTGCCGCAATTGGATGCCATCCAGGGAGCCATGTTAA *F CGAATTGTTTATGTGTTATACCCGGTATTTTAGGCTTGATGTCACGCACC *F AGCAAAGAGGGTAAACGTTTTGCCAAAATGTTAATTGATTTAGTGGCAGT *F AGCTGCTCAGGTAACCGGTTTTGTTATATGGCCTCTATTGGAGAATCGTA *F AAGAGTTGTGGGTTATACCAGTGGCTTGTCTAATGGTCTCGTGTGGCTGG *F TGGGAGAATTATGTTACTTCACAATCTTCATTTGCCTTAATAAGATCTAT *F GGGCCGAGTTAAGGATGATTTGAAATATACCAGATATTTCAGTCACATGT *F TTTTATCTATATGGAAAATTTCCCTATTCTTGTGTTCCGTGCTGTTGATC *F TATTGGATTCAGGGTGAGGAACCAGGCAATATCTTTGGTTTATTCAGTGA *F TGCTTTTGGGCCTCATAAGATATCTGTAGATGAATTGGCTACCACCTTGA *F CCAACAGTTTACCCGATACCCTAGATGCTGCCAATATAGATAGTATTGAT *F ATCGAGGCTTCCTATAATACAGTGGTCTATGTTTTGCTGATCCAAATATT *F CGGCGCTTATCTTTGTTATATTTTTGGCAAATTCGCTTGTAAGATTTTAA *F TCCAAGGTTTCAGTTATGCCTTTCCGGTCAGTTTGACCGTACCCGTTGCT *F GTGTCTTTGTTGATAGCGGCTTGCGGTATTAGAATAGATGATCCCTGCTT *F CTTCCATGATACCATACCAGATTATTTGTTCTTTACAAGTCCCTCCAACT *F TTAGATTTAATGATTTTGTTACTAATCAAATGGCTTGGGCTTGGATCTTG *F TGGTTATTGTCACAGACCTGGATTGGCTTACATATATGGACACCTAAATG *F TGAACGTTTAGCCACAACAGAGAAATTATTTGTAAAACCCATGTATTGCT *F CTCTGCTGATCGATCAATCTATGGCCATGAATAGGAGGAGAGATGATCAG *F GCTGATGTGAAAACTGAGGATAACAATTTAATTTTAAAACAATTTAACTA *F TTTACAGGATCTTTCGGAAATTGAAAAGGAAAAGGGAGACGAATACTATG *F AAACCATTTCTGTACACACCGATGGCTCGGCCATACAAAGTAAGCCCACC *F ATTAAGTCTTCCGATCATATAACCAGAATCTATTCTTGCGCTACTATGTG *F GCATGAAACAAAAGATGAGATGATAGAGTTTTTAAAGAGTATCATGCGTA *F TGGATGAAGATCAGTGTGCTCGTAGAGTTGCTCAGAAGTATCTGAGAATT *F GTCGATCCCGATTATTATGAATTTGAAGCTCACACTTTCTTCGATGATGC *F TTTTGAAATTTCCGATCACAGTGATGACGATATCCAGGTAAATCGTTTCG *F TCAAACTTTTGGTAGCTACCATGGATGATGCCGCCTCTGAAATTCATCAG *F ACCAGAATACGTTTGCGTCCGCCCAAGAAATATCCCACACCCTATGGTGG *F TCGTTTGGTTTGGACTCTACCCGGTAAAACAAAATTCATTGCTCATTTAA *F AAGACAAAGATCGTATTCGTCATCGTAAACGTTGGTCTCAAGTCATGTAC *F ATGTACTATCTGTTGGGTCATCGTCTCATGGAACTGCCTATACCCGAGGA *F TCGTAAAGATACCATAGCCGAAAATACTTATCTCTTGACCTTGGATGGTG *F ATATTGATTTCAAACCAAATGCTGTAACTTTACTGGTGGATTTGATGAAA *F AAGAACAAGAATTTAGGTGCTGCCTGTGGTCGCATTCATCCTGTGGGCTC *F TGGTCCTATGGTGTGGTATCAGCTGTTCAAATATGCTATTGGTCATTGGC *F CTCAAAAGGCCACCGAACATATGATTGGTTGTGTACTTTGTAGTCCCGGT *F TGTTTCTCACTTTTCCGTGGCAAAGCCTTGATGGACGATAATGTTATGAA *F GAAATATACCACGCAATCCGATGAAGCTCGTCATTATGTACAATACGATC *F AGGGAGAAGATCGTTGGTTGTGTACTTTGCTTTTGCAGCGTGGTTATCGT *F GTCGAATATTCAGCTGCCTCCGATGCCTACACTCACTGTCCCGAAGGTTT *F TAATGAATTCTACAATCAACGTCGTCGCTGGGTACCCTCGACCATTGCCA *F ATATTATGGATTTGCTAGGCGATGCCAAACGTACGATTAAGATTAATGAC *F AACATTTCACTGCTCTATATCTTCTATCAAATGATGTTAATGGGTGGTAC *F TATTCTTGGACCTGGTACGATTTTCCTTATGTTGGTGGGTGCTTTTGTGC *F TGCCTTCCGTATCGACAATTGGACTTCTTTCCATTACAATATCAATTCCG *F ATTCTGGGCTTCATGTTTATCTGTTTCACATGTAAGGCCAATATACAACT *F GTTTGTGGCTCAGGTTCTGTCAACGGCATATGCTCTCATTATGATGGCTG *F TCATTGTGGGTACGGCTTTGCAGTTAGGCGAGGATGGTATAGGTTCTCCG *F TCGGCCATTTTCTTGATATCTATGGTGGGGTCGTCCTTTATAGCGGCGTG *F TTTACATCCGCAGGAGTTTTGGTGTATATCAGCGGGTCTCATCATATTTA *F CGTGTCCAAACCAAACGACCACCATAATCAC *F Browser: Mozilla/4.7 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0129251 *a Rawson *b R. *t 2000.5.31 *T personal communication to FlyBase *u *F From rawson@utsw.swmed.edu Wed May 31 20:37:36 2000 *F Subject: Df(3L)ZN47 *F To: flybase-help@morgan.harvard.edu *F Greetings, *F I have received a couple of queries lately asking for my Df(3L)64C-65C line *F which is listed in flybase as having no stocks available. This deficiency *F is erstwhile stock ZN47 which has a separate entry in fly base. There is no *F cross reference or link, and data pertinent to ZN47 is listed only under *F Df(3L)64C-65C. I suppose I am the only person who knows that the two events *F are one in the same. Had I not my 1991 lab notes I am not sure that I would *F know it. *F I believe that you should update one record or the other and delete the *F remaining one. *F Thanks for your good efforts. *F Yours truly, *F Robert B. Rawson *F Assistant Professor *F Room L5.276 *F Department of Molecular Genetics *F University of Texas Southwestern Medical Center *F 5323 Harry Hines Blvd *F Dallas TX 75325-9046 *F Office: (214) 648-8663 *F Lab: (214) 648-8679 *F Fax: (214) 648-8804 # *U FBrf0129255 *a Bellen *b H.J. *c C.J. *d O'Kane *e C. *f Wilson *g U. *h Grossniklaus *i R.K. *j Pearson *k W.J. *l Gehring *t 2000 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Wed May 31 17:17:59 2000 *F Subject: lacZ expression patterns *F To: flybase-updates@morgan.harvard.edu *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Bellen, H.J., O'Kane, C.J., Wilson, C., Grossniklaus, U., Pearson, R.K., Gehring, *F W.J. *F To: HHMI P Stock Center *F Dated: Unknown *F Background: The following information was included in a preprint of Bellen et al., 1989, Genes Dev. 3:1288--1300 *F (FBrf0049801) and/or in tables of staining pattern information provided when the stocks were donated to the HHMI P *F Stock Center (subsequently transferred to the Bloomington Stock Center), but apparently did not appear in the *F published version of the paper. *F FlyBase has an insertion record and lacZ allele for each of these, but no lacZ expression pattern is associated *F with the lacZ allele. In one case, a mutant allele also needs to be created and the insertion renamed accordingly. *F Information communicated: *F 1. P{lArB}A106.1F1, FBti0005693 *F Gene symbol: l(1)A106.1F1 *F Gene synonym: A106.1F1.L *F Map position: 7C (based on in situ of associated insertion) *F Allele symbol: l(1)A106.1F1A106.1F1 *F Allele phenotype: recessive lethal *F Other info: Lethality is complemented by *F Tp(1;3)sn13a1 *F Tp(1;2)sn+72d *F Associated insertion: P{lArB}l(1)A106.1F1 (new symbol for FBti0005693) *F Insertion symbol: P{lArB}l(1)A106.1F1 (new symbol for FBti0005693) *F Associated allele: Ecol\lacZA106.1F1 (FBal0040637) *F lacZ staining pattern: *F embryo: preblastoderm *F ovary: nurse cells, stage 10 (faint) *F oocyte nucleus, stage 14 *F 2. P{lArB}A103.1M2 (FBti0005692) *F Associated allele: Ecol\lacZA103.1M2 (FBal0040636) *F lacZ staining pattern: *F embryo: anterior epidermis *F segment boundry *F midgut *F proventriculus *F denticle belts?, especially A2-A8/9 *F ovary: no staining *F 3. P{lArB}A217.1M2 (FBti0005703) *F Associated allele: Ecol\lacZA217.1M2 (FBal0040669) *F lacZ staining pattern: *F embryo: gonad, stage 17 *F ovary: no staining *F 4. P{lArB}A193.5F3 (FBti0005702) *F Associated allele: Ecol\lacZA193.5F3 (FBal0040660) *F lacZ staining pattern: *F ovary: germarium, region 2 *F nurse cells, stage 1 *F ooplasm *F follicle cells, stage 1 *F 5. P{lArB}B26.1M3 (FBti0005744) *F Associated allele: Ecol\lacZB26.1M3 (FBal0040861) *F lacZ staining pattern: *F third instar larva: CNS *F eye-antennal disc *F leg disc *F wing disc *F 6. P{lArB}A100.1M3 (FBti0005690) *F Associated allele: Ecol\lacZA100.1M3 (FBal0040634) *F lacZ staining pattern: *F embryo: epidermis, T2 and T3, anterior to segmental boundary *F posterior spiracles *F ovary: no staining *F 7. P{lArB}A143.1F3 (FBti0005699) *F Associated allele: Ecol\lacZA143.1F3 (FBal0040648) *F lacZ staining pattern: *F embryo: posterior spiracle, stage 13 *F Malpighian tubules, stage 12 *F head, labial segment?, antennomaxillary complex? *F ovary: no staining *F 8. P{lArB}A102.2F3 (FBti0005691) *F Associated allele: Ecol\lacZA102.2F3 (FBal0040635) *F lacZ staining pattern: *F embryo: repeated pattern: anterior to cephalic furrow and dorsolateral posterior epidermis *F of each segment *F CNS (all), stage 17 *F brain, stage 12 *F dorsal nerve cord, stage 12 *F PNS, *F head *F ovary: no staining # *U FBrf0129256 *a Roote *b J. *t 2000.6.5 *T personal communication to FlyBase *u *F To: rd120@mole.bio.cam.ac.uk, Aubrey de Grey *F From jr32@mole.bio.cam.ac.uk Mon Jun 05 20:03:55 2000 *F Subject: updates *F Hello folks, *F a few updates for abs in the Adh region \- probably not the last. *F John *F Df(2L)b88c25 deletes kuz-osp *F In(2L)b88e16 deletes kuz-osp (Adh not tested) (i.e. must a 3 break event) *F Df(2L)b89e96 deletes kuz-l(2)34Fa *F Df(2L)b89e80a deletes kuz-vasa *F Df(2L)b89e88a deletes kuz-l(2)35Ea, l(2)35De, l(2)35Dh *F Df(2L)b89e88b deletes kuz-osp (Adh not tested) *F Df(2L)b89e64b deletes kuz-l(2)34Fa *F Df(2L)b89e68 deletes kuz-osp (Adh not tested) *F Df(2L)b89e72 deletes kuz-l(2)34Fa *F In(2L)b88e45 deletes l(2)34Db-RpII33 *F Df(2L)b88g83 deletes l(2)34Db-RpII33 *F T(2;3)88g68 = b88g68 deletes l(2)34Db-osp (Adh not tested) *F In(2L)b89e100 deletes l(2)34Db-b *F Df(2L)b89e8 deletes Sos-l(2)34Fa *F T(2;3)b89e12 deletes Sos-MtPolB (=l(2)34De). *F Df(2L)b88g26 deletes l(2)34Db-l(2)34Fd *F Df(2L)b89e80b breaks within (is hypomorphic for) l(2)34Db and deletes *F Sos-b (which doesn't explain why the cytology is reported to be *F 34D4;35B10 \- we have not checked it.) *F Df(2L)b88f40 deletes l(2)34Db-l(2)34Fa *F In(2L)b91l9 breaks within (is hypomorphic for) l(2)34Db and deletes Sos-b *F Df(2L)b87e152 deletes Sos-b *F T(Y;2)b88g22, T(2;3)b88g22 deletes Sos-b # *U FBrf0129257 *a Brand *b A. *t 2000.6.5 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jun 05 20:29:54 2000 *F To: Rachel Drysdale (Genetics) *F Subject: P{UAS-tau-lacZ.B}2 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{UAS-tau-lacZ.B}2 insertion *F The following information accompanied a stock donated by Andrea Brand, *F Wellcome/CRC Institute (5/00). *F The P{UAS-tau-lacZ.B}2 insertion is a homozygous viable and fertile second *F chromosome insertion. *F The construct and its transformation were described in Hidalgo et al. 1995 *F Development 121(11):3703--3712 (FBrf0084024). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129258 *a Dickson *b B. *t 2000.6.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jun 08 21:56:20 2000 *F Envelope-to: rd120@gen.cam.ac.uk *F To: Rachel Drysdale (Genetics) *F Subject: yd2 *F From: Kevin Cook, Bloomington Stock Center *F Subject: yd2 allele *F We received stocks from Barry Dickson, University of Vienna, marked with *F yd2. Its phenotype is like y1, i.e. yellow bristles, wings, tarsal *F claws and cuticle. Origin unknown. *F Please create an allele entry. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129259 *a Eeken *b J. *t 2000.6.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jun 08 22:03:25 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: drw2 *F From: Kevin Cook, Bloomington Stock Center *F Subject: drw2 allele *F The drw2 allele was isolated in the lab of Jan Eeken, Leiden University, *F and was sent to us by him. It is homozygous viable with wings that tend to *F curl down. Mutagen unknown. *F Please create an allele entry. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129260 *a Laverty *b T. *t 2000.6.8 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Jun 08 23:22:38 2000 *F Subject: P{neoFRT}73D *F To: flybase-updates@morgan.harvard.edu *F From: Kathy Matthews matthewk@fly.bio.indiana.edu *F Personal communication from: Todd Laverty, Berkeley Drosophila Genome Project *F To: Bloomington Drosophila Stock Center *F Dated: 26 February 1996 *F Background: Two stocks with P{neoFRT} insertions at 80B, donated to the *F Bloomington *F collection by T. Xu and G. Rubin, were found to have an additional P{neoFRT} *F insertion. *F There is currently no record of this insertion in FlyBase. *F Information communicated: *F Insertion Symbol Insertion Site *F P{neoFRT}73D 73D # *U FBrf0129261 *a Roote *b J. *t 2000.6.13 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Tue Jun 13 19:18:51 2000 *F To: rd120@mole.bio.cam.ac.uk *F Subject: new P derivatives *F Dear Rachel, *F Here is a list of deletions that we have made over the last few years *F by P-element excision and male recombination - some very informative, *F some less so. All induced with &Dgr;2-3. *F Fondest regards, *F Rootles *F (pers comm that if you dare) *F k07245 BG:DS00797.1 *F k07245-rv1 kuz-, l(2)34Db+. *F rN149 BG:DS08220.1 *F rn149-rv3 RpII33+, Ance-, j-, rk-, l(2)34Fa-, wb-, *F ms(2)34Fe+ *F k11509 smi35A *F k11509-rv18 wb- *F k08712 *F k08712-rv2 l(2)34Fd-, semi-lethal, short bristles *F k08712-rv21 l(2)35Aa-, lethal, rescued by 35Aa 5kb rescue fragment *F k08712-rv34 l(2)34Fd-, lethal *F EP(2)2039 elB *F EP(2)2039-rv4 wb+, ms(2)34Fe-,l(2)34Fc-, l(2)34Fd-, *F l(2)35Aa-, elB-, pu+ *F EP(2)2039-rv3 elB-, pu- *F k13218 osp *F 225 w- revertants 7 were osp-, 3 were noc+/- osp- *F k11524 l(2)35Bb *F 2 out of 7 excisions revert the lethality. *F k08808 l(2)35Bc *F 121 excisions, 37 lethal over Df(2L)nBR107 (i.e. 84 precise *F excisions): 31 imprecise excisions l(2)35Bc-, 5 deletions: *F k08808-rv25 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+ *F k08808-rv52 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+ *F k08808-rv57 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+ *F k08808-rv63 l(2)35Be+, l(2)35Bc-, l(2)35Bd-, l(2)35Bg+ *F k08808-rv70 l(2)35Bf+, l(2)35Be-, l(2)35Bc-, l(2)35Bd+ *F Therefore the gene order is 35Bb, 35Bf, 35Be, 35Bc, 35Bd. *F PZ05271 l(2)35Ea *F 1 excision out of 14 (from Julie Gates, Utah) is lethal. *F PZ05271-rv54 l(2)35Ea- *F k09834 PRL-1 *F k09834rv1 Ca-a1+, twe-, crp-, l(2)35Fb+ *F k09834rv2 Ca-a1+, twe-, crp+ *F k17027 cact *F k17027rv1 fzy+, cact- (fs over H60-3), l(2)35Fe+ *F k17027rv2 cact+, l(2)35Fe-, chif+ *F k17027rv3 fzy+, cact- (fs over cactAR51.4), l(2)35Fe+ *F k04216 chif *F RN2 chifWD18 *F k04216-rv1 female sterile female fertile! *F k04216-rv2 female sterile female sterile *F k04216-rv3 female sterile female sterile *F k08106 multiple insert in 35F *F 14 excisions, selected as w-: 10 lethal, 4 semi-lethal and fs over *F RN2, 6 retain weak w+. *F k08106-rv4 cni+, fzy-, cact-, l(2)35Fe-, chif-, *F l(2)35Fg-, dac+; w+ (weak) *F k08106-rv6 cni+, fzy-, cact-, l(2)35Fe-, chif-, *F l(2)35Fg-, dac+; w- *F k08106-rv15 cni+, fzy-, cact-, l(2)35Fe-, chif-, *F l(2)35Fg-, dac+; w- *F Male recombinants - induced over dpov1 b1 cn1 bw1 *F k07245 BG:DS00797.1 *F k07245-mr2 b kuz+, l(2)34Db- - Ance-Š. *F k07245-mr9 dp kuz+/- *F k07245-mr11 b kuz+, l(2)34Db- - wb- *F k07245-mr28 dp kuz+/-, l(2)34Db+ *F k07245-mr31 b kuz+, l(2)34Db- - ms(2)35Ci-, esg+ *F k07245-mr32 b kuz+, l(2)34Db- - gft-, rd+ *F k05007 CycE *F k05007-rv1 cn bw CycE+, l(2)35Di-, l(2)35Df+ *F mr1 cn bw lace+, CycE-, l(2)35Di-, l(2)35Df+ *F mr2 cn bw lace+, CycE-, l(2)35Di-, *F l(2)35Df-, Gli-, l(2)35Ea+ *F mr3 cn bw lace+, CycE-, l(2)35Di-, *F l(2)35Df-, Gli-, l(2)35Ea+ *F mr6 cn bw lace+, CycE-, l(2)35Di-, *F l(2)35Df-, Gli-, l(2)35Ea+ *F mr9 cn bw lace+, CycE-, l(2)35Di-, *F l(2)35Df-, Gli-, l(2)35Ea+ *F mr13 cn bw lace+, CycE-, l(2)35Di-, *F l(2)35Df-, Gli-, l(2)35Ea+ *F mr15 cn bw lace+, CycE-, l(2)35Di-, *F l(2)35Df-, Gli-, l(2)35Ea-, l(2)35Dh-, l(2)35De- *F mr19 dp b vasa+, stc- - CycE-, l(2)35Di+ *F PZ07130 ck *F PZ07130-mr1 dp b kuz- - ck-, l(2)35Cf+ *F PZ07130-mr2 cn bw Su(H)+, ck- - vasa-, stc+ *F PZ07130-mr3 cn bw Su(H)+, ck-, l(2)35Cf+ *F PZ07130-mr5 cn bw Su(H)+, ck- - esg-, wor+ *F PZ07130-mr7 cn bw Su(H)+, ck- - vasa-, stc+ *F PZ07130-mr8 cn bw Su(H)+, ck-, l(2)35Cf+ *F PZ07130-mr9 dp b l(2)35Bg- - ck-, l(2)35Cf+ *F PZ06430 gft *F PZ06430-mr1 cn bw l(2)35Cc+, gft-, ms(2)35Ci+ *F mr2 dp b l(2)35Cc+, gft-, ms(2)35Ci+ *F mr3 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr4 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr5 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr7 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr8 cn bw l(2)35Cc+, gft-, ms(2)35Ci+ *F mr9 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr10 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr11 dp b l(2)35Cc+, gft-, ms(2)35Ci+ *F mr12 cn bw l(2)35Cc+, gft-, ms(2)35Ci-, esg+ *F mr13 cn bw l(2)35Cc+, gft-, ms(2)35Ci+ *F mr14 dp b rd+, l(2)35Cc, gft-, ms(2)35Ci+ *F PZ05271 l(2)35Ea *F PZ05271-mr15 dp b Gli+, l(2)35Ea-, l(2)35De-, *F l(2)35Dh-, BicC+ *F mr33 cn bw CycE+, l(2)35Di-, l(2)35Df-, *F Gli-, l(2)35Ea+ *F mr45 dp b l(2)35Ea- *F Note: The dp b deletion should go distally, cn bw proximally. However 35Di, *F 35Df and Gli are definitely distal of 35Ea. *F k09033 Gli *F k09033-mr9 dp b CycE+, l(2)35Di- - Gli-, l(2)35Ea+ *F k09033-mr19 dp b vasa+, stc- - Gli-, l(2)35Ea+ *F k14608 l(2)35Fe *F k14608-mr4 dp b l(2)35Fe- *F mr6 dp b l(2)35Di- - l(2)35Fe- *F mr15 dp b fzy- - l(2)35Fe- *F mr16 dp b twe- - l(2)35Fe- *F mr23 cn bw l(2)35Fe- - chif- *F mr27 dp b cact- - l(2)35Fe- *F mr29 dp b l(2)35Ff- - l(2)35Fe *F Double-P male recombinants - induced over dpov1 b1 cn1 bw1 *F k07245 - k05605 BG:DS00797.1 - RpII33 *F k07245L k05605R-mr1 kuz - RpII33 *F k07245L k05605R-mr3 kuz - RpII33 *F k07245L k05605R-mr5 l(2)34Db - MtPolB *F k07245L k05605R-mr9 l(2)34Db - MtPolB *F k07245L k05605R-mr6 l(2)34Db - l(2)34Fa *F k07245L k05605R-mr8 l(2)34Db - l(2)34Fa *F k07245L k05605R-mr10 l(2)34Db - RpII33 *F k07245L k05605R-mr12 l(2)34Db - RpII33 *F k07245L k05605R-mr7 l(2)34Db - RpII33 *F k07245 - k00811 BG:DS00797.1 - l(2)34Fa *F k07245L k00811R-mr2 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr3 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr4 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr5 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr11 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr13 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr14 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr6 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr15 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr7 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr8 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr10 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr16 l(2)34Db - l(2)34Fa *F k07245L k00811R-mr17 l(2)34Db - l(2)34Fa *F w11P - PZ07130 rk - ck *F rkw11PL PZ07130R-mr1 rk - ck *F rkw11PL PZ07130R-mr2 rk - ck *F rkw11PL PZ07130R-mr3 rk - ck *F rkw11PL PZ07130R-mr4 rk - ck *F rkw11PL PZ07130R-mr5 rk - ck *F rkw11PL PZ07130R-mr6 rk - ck *F rkw11PL PZ07130R-mr7 rk - ck *F rkw11PL PZ07130R-mr8 rk - ck *F rkw11PL PZ07130R-mr9 rk - ck *F rkw11PL PZ07130R-mr10 rk - ck *F rkw11PL PZ07130R-mr11 rk - ck *F rkw11PL PZ07130R-mr12 rk - ck *F rkw11PL PZ07130R-mr13 rk - ck *F rkw11PL PZ07130R-mr14 rk - ck *F rkw11PL PZ07130R-mr15 rk - ck *F rkw11PL PZ07130R-mr16 rk - ck # *U FBrf0129262 *a Collier *b S. *c J. *d Roote *t 2000.6.19 *T personal communication to FlyBase *u *F >From jr32@mole.bio.cam.ac.uk Mon Jun 19 13:39:29 2000 *F To: rd120@mole.bio.cam.ac.uk *F Subject: Re: this and that *F Dear Rachel, *F I can confirm that Simon's original frtz list has frtz19 as *F 22A2.3;22B1. However I also found the primary data sheet (an *F historic scrap of aged vellum) on which Simon has written *F 22A2-3;22B3. *F Glad to be able to clear up that one. *F J *F >On the subject, I noticed that the entry for frtz19 in Flybase *F >quotes the deletion as Df(2L)frtz19 22A2-3;22B7 whereas my original *F >cytology gave the proximal break as 22B1. Was there other *F >information? I mentioned this to Rach and she said you and me would *F >have to write her a little letter if we want her to change it. *F > *F >Thanks again, Simon *F >From jr32@mole.bio.cam.ac.uk Mon Jun 19 14:35:12 2000 *F To: rd120@mole.bio.cam.ac.uk *F Subject: Fwd: Re: this and that *F However: *F >From: 'Simon Collier' *F >To: John Roote *F >Date: Mon, 19 Jun 2000 14:25:32 GMT+1BST *F >Subject: Re: this and that *F > *F >I have my own fragment of parchment here that says 22B1, I guess *F >we'll have to leave it to the scholars to figure out which was the *F >original source. Still, it's not 22B7 and for some reason I was happy *F >with 22B1 from early on...... *F > *F >Dr. Simon Collier *F >University of Manchester *F >School of Biological Sciences *F >2.205 Stopford Building *F >Manchester M13 9PT, UK *F >Tel: 0161 275 3879/5061 *F >Fax: 0161 275 5082 *F >E-mail: simon.collier@man.ac.uk # *U FBrf0129263 *a Dura *b J.M. *t 2000.6.15 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jun 15 23:18:31 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Fauvarque and Dura Polycomb enhancers and suppressors *F From: Kevin Cook, Bloomington Stock Center *F Subject: Fauvarque and Dura Polycomb enhancers and suppressors *F I have been corresponding with Jean-Maurice Dura, CNRS Montpellier, *F concerning stocks described in his paper Fauvarque and Dura, Genes and *F Development 7:1508-20, 1993 (FBrf0058522). The following FlyBase entries *F need to be created or modified based on this correspondence. *F 1. E(Pc)14B *F a. The locus E(Pc)14B (FBgn0013933) was defined by the insertion *F P{418}E(Pc)14BT2.2.31 (FBti0012376/FBal0033632). Table 2 of the paper *F gives its position as 14B; however, Dura says that the insertion site is *F really 17B based on in situ hybridization and sequence data after PCR *F rescue. Consequently, the locus name should be changed to E(Pc)17B. *F b. The paper describes two alleles of E(Pc)17B: the original P{418} *F insertion allele T2.2.31 and an excision allele 2.2.31. There needs to *F be a FB allele entry created for 2.2.31. *F c. According to Dura's correspondence, the original E(Pc)17BT2.2.31 *F insertion allele did not act as an Enhancer of Polycomb, but the P excision *F allele E(Pc)17B2.2.31 did. It is the effect of E(Pc)17B2.2.31 that is *F described in Table 3 of the paper. *F d. Another excision of P{418}E(Pc)17BT2.2.31 not described in the paper, *F E(Pc)17B147, behaves the same as E(Pc)17B2.2.31. An allele entry *F should be created for E(Pc)17B147, since this allele will be in the *F Bloomington stock collection. *F 2. E(Pc)35CD and su(Pc)84D *F The E(Pc) and Su(Pc) names were not given in the paper. The mutations were *F probably assigned these names based on the effects described in Table *F 3. Unfortunately, two of the loci were misnamed: *F P{418}E(Pc)35CDT41.2 (FBgn0013934/FBal0033633/FBti0012373) is really a *F dominant suppressor of Pc, so its name should be changed to *F P{418}Su(Pc)35CDT41.2. *F P{418}su(Pc)84DT66.1 (FBgn0014389/FBal0035735/FBti0012375) is really a *F recessive enhancer of Pc, so its name should be changed to *F P{418}e(Pc)84DET66.1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129264 *a Laverty *b T. *t 2000.4.6 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Mon Jun 19 16:35:35 2000 *F Subject: in situ site correction for l(3)04837 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Todd Laverty, Berkeley Drosophila Genome Project *F To: Bloomington Drosophila Stock Center *F Dated: 6 April 2000 *F Information communicated: *F A reexamination of the in situ localization of l(3)04837 places the insertion *F at 88D5-6 rather than the *F originally reported 85D5-6. # *U FBrf0129265 *a Luo *b L. *t 2000.6.26 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jun 26 16:59:21 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GawB}OK107 insertion *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{GawB}OK107 insertion *F According to Liqun Luo, Stanford University, P{GawB}OK107 (FBti0004170) is *F inserted on the fourth chromosome near eyeless. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129266 *a Kaufman *b T. *t 2000.6.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jun 29 20:34:09 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{lacW}arrk08131 *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{lacW}arrk08131 *F The following information accompanied a stock donated by Thom Kaufman, *F Indiana University (6/00). The lethal P insertion *F P{lacW}l(2)k08131k08131 (FBal0064341) is an allele of arrow. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129267 *a Roote *b J. *t 2000.7.3 *T personal communication to FlyBase *u *F From jr32@mole.bio.cam.ac.uk Mon Jul 03 15:28:32 2000 *F To: rd120@mole.bio.cam.ac.uk, Aubrey de Grey *F Dear Rachel/Aubrey, *F I've just scampered through the first 100 Dfs from a search of Dfs *F in the 34-36 region. The following are wrong for various reasons \- *F mostly new-ish data. *F Cheers, *F John *F Df(2L)64j *F nub << bk1 << l(2)34Db << TfIIS << bk2 << vas *F kuz << bk1 << l(2)34Db << TfIIS << bk2 << vas *F Df(2L)A217 *F wb << bk1 << l(2)34Fc << Adhr << bk2 << l(2)35Bb *F wb << bk1 << ms(2)34Fe << Adhr << bk2 << l(2)35Bb *F Df(2L)b88f40 *F bk1 << b << bk2 *F kuz << bk1 << l(2)34Db << l(2)34Fa << bk2 << wb *F Df(2L)b91l5 *F bk1 << b << bk2 *F kuz << bk1 << l(2)34Db << rk << bk2 << l(2)34Fa *F Df(2L)b75 *F kuz << bk1 << l(2)34Db << wb << bk2 << l(2)34Fc *F kuz << bk1 << l(2)34Db << wb << bk2 << ms(2)34Fe *F Df(2L)b77c *F bk1 << l(2)34Db << l(2)34Fa << bk2 << pu *F kuz << bk1 << l(2)34Db << l(2)34Fa << bk2 << wb *F Df(2L)b78j *F kuz << bk1 << l(2)34Db << wb << bk2 << l(2)34Fc *F kuz << bk1 << l(2)34Db << wb << bk2 << ms(2)34Fe *F 'Df(2L)b79a3' *F bk1 hits b << bk2 hits l(2)35Ea *F does this imply a deletion from b \-> 35Ea? Actually it's not a *F deletion, just b- and 35Ea-. *F Df(2L)b79b4 *F kuz << bk1 << l(2)34Db << wb << bk2 << l(2)34Fc *F kuz << bk1 << l(2)34Db << wb << bk2 << ms(2)34Fe *F Df(2L)b80k *F bk1 << l(2)CA61 << l(2)35Bd << bk2 << l(2)35Bg *F kuz << bk1 << l(2)34Db << l(2)35Bd << bk2 << l(2)35Bg *F Df(2L)b81a2LA80R *F Sos << bk1 << b << pu << bk2 << elA *F In(2LR)b81a2LDTD43R *F Sos << bk1 << b << noc << bk2 << osp *F Df(2L)b81l42 *F kuz << bk1 << l(2)34Db << DNApol-gamma35 << bk2 << Ance *F kuz << bk1 << l(2)34Db << RpII33 << bk2 << Ance *F Df(2L)b82a3 *F Sos << bk1 << b << tam << bk2 << Orc5 *F Sos << bk1 << b << tam << bk2 << sop2 *F Df(2L)b83d29a *F kuz << bk1 << pu << bk2 << elA << bk3 *F kuz << bk1 << l(2)34Db << pu << bk2 << elA << bk3 *F Df(2L)b83l1 *F kuz << bk1 << l(2)34Db << DNApol-gamma35 << bk2 << Ance *F kuz << bk1 << l(2)34Db << RpII33 << bk2 << Ance *F Df(2L)b84a4 *F kuz << bk1 << b << noc << bk2 << osp *F kuz << bk1 << l(2)34Db << noc << bk2 << osp *F Df(2L)b84a8 *F b << bk1 << l(2)34Db << noc << bk2 << osp *F kuz << bk1 << l(2)34Db << noc << bk2 << osp *F Df(2L)b84a9 *F l(2)34Db << bk1 << Sos << DNApol-gamma35 << bk2 << Ance *F l(2)34Db << bk1 << Sos << RpII33 << bk2 << Ance *F Df(2L)b85b1 *F kuz << bk1 << l(2)34Db << DNApol-gamma35 << bk2 << Ance *F kuz << bk1 << l(2)34Db << RpII33 << bk2 << Ance *F Df(2L)b85b2 *F Sos << bk1 << b << tam << bk2 << Orc5 *F Sos << bk1 << b << tam << bk2 << sop2 *F Df(2L)b85f1 *F kuz << bk1 << l(2)34Db << wb << bk2 << l(2)34Fc *F kuz << bk1 << l(2)34Db << wb << bk2 << ms(2)34Fe *F Df(2L)b88b42 *F Sos << bk1 << b << tam << bk2 << Orc5 *F Sos << bk1 << b << tam << bk2 << sop2 *F Df(2L)b88c25 *F bk1 << l(2)CA61 << bk2 << l(2)35Bb *F bk1 << kuz << osp << bk2 << l(2)35Bb *F Df(2L)b88g83 *F kuz << bk1 << l(2)34Db << DNApol-gamma35 << bk2 << Ance *F kuz << bk1 << l(2)34Db << RpII33 << bk2 << Ance *F Df(2L)b89e68 *F bk1 << b << bk2 *F kuz << bk1 << l(2)34Db << osp << bk2 << l(2)35Bb *F Df(2L)b89e72 *F bk1 << b << bk2 *F kuz << bk1 << l(2)34Db << l(2)34Fa << bk2 << wb *F Df(2L)b89e8 *F bk1 << b << bk2 *F l(2)34Db << bk1 << Sos << l(2)34Fa << bk2 << wb *F Df(2L)b89e80a *F bk1 << b << bk2 *F bk1 << kuz << vasa << bk2 << stc *F Df(2L)b89e80b *F bk1 << b << bk2 *F bk1 within l(2)34Db << b << bk2 << tamas # *U FBrf0129268 *a Laverty *b T. *t 2000.7.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jul 06 17:23:31 2000 *F To: Rachel Drysdale (Genetics) *F Subject: P{lacW}l(2)k17019b insertion site *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{lacW}l(2)k17019b insertion site *F According to Todd Laverty (7/3/2000), UC Berkeley/BDGP, P{lacW}l(2)k17019b *F is inserted at 49E1-2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129269 *a Johnson *b W. *t 2000.7.6 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jul 06 18:43:02 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: l(3)65AEaN85 *F From: Kevin Cook, Bloomington Stock Center *F Subject: l(3)65AEaN85 *F The following information accompanied stocks donated to the Stock Center by *F Wayne Johnson, University of Iowa (6/00). The l(3)65AEaN85 mutation was *F isolated in the EMS mutagenesis screen for lethals failing to complement *F Df(3L)W5.4 described in Anderson et al. 1995, Genes Dev. 9(1):123--137 *F (FBrf0079852). It complemented all other lethals identified in the screen. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129566 *a Bloomington Drosophila Stock Center *b ?. *t 2000.8.17 *T personal communication to FlyBase *u *F Date: Wed, 09 Feb 2000 16:37:54 -0500 *F To: flybase-updates@morgan.harvard.edu *F *F From: Kevin Cook, Bloomington Stock Center *F *F Subject: Revised insertion site for P{UAS-GFP.nls}8 *F *F Information that accompanied the donation of our stock *F *F 4776 w[1118]; P{w[+mC]=UAS-GFP.nls}8 *F *F indicated that P{UAS-GFP.nls}8 (FBti0012493) is inserted in chromosome 2. *F Information from Stock Center users and my own remapping of the insertion *F indicate that it is really on chromosome 3. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0129567 *a Misra *b S. *t 2000.7.28 *T personal communication to FlyBase *u *F Here are the matches of the nc2 class to CGs where possible, and to the *F scaffolds, where there was not CG. *F *F cheers, *F sima *F *F > nc2: genes with no SWP but with either TREMBL or PIR (47). Quite a *F > few of these have a PIR entry and no nucleic acid one -- no idea *F > what that means. Again some of these (commented) have already been *F > looked at. *F *F > X-Sun-Data-Name: nc2 *F *F > Acp98AB - Cat 1 residue *F =new exon in first intron of CG12879 CT32023 FBan0012879 FBgn0039540 *F >gb|AE003762.1|AE003762 Drosophila melanogaster genomic scaffold *F 142000013386035 section 87 of *F 105, complete sequence *F Length = 230995 *F *F Score = 52.3 bits (123), Expect = 2e-07 *F Identities = 24/26 (92%), Positives = 24/26 (92%) *F Frame = +2 *F *F Query: 2 EFPNPVLSRIGRSLRTNKGTHYQRMT 27 *F E NPVLSRIGRSLRTNKGTHYQRMT *F Sbjct: 33911 EVTNPVLSRIGRSLRTNKGTHYQRMT 33988 *F *F Database: Drosophila Genome *F Posted date: Apr 10, 2000 4:44 PM *F Number of letters in database: 122,680,987 *F Number of sequences in database: 1181 *F *F > Acph-1 *F (438aa) *F *F =CG7899|FBan0007899|CT6619|FBan0007899 last_updated:000321 (438aa) *F 95% id over 438aa *F *F > anon-63BC-T3 *F O18384(31aa) by BLASTP against predicted proteins at BDGP *F =CG14965|FBan0014965|CT34811|FBan0014965 last_updated:000321(537aa) *F *F Score = 65 (22.9 bits), Expect = 0.049, P = 0.048 *F Identities = 11/11 (100%), Positives = 11/11 (100%) *F *F Query: 21 MHFFKFPVKDP 31 *F MHFFKFPVKDP *F Sbjct: 1 MHFFKFPVKDP 11 *F *F missing first 20aa in CG *F *F > anon-67Ea - Cat 9 residue, BLASTN finds CGless seq *F CAA48385(170aa) by BLASTP against predicted proteins at BDGP *F =no protein in GadFly *F GenBank:X68309(2578bp) by BLASTN against all at BDGP *F =gb|AE003547|Drosophila melanogaster genomic scaffold, 142000013386050 *F section 34 of 54, complete sequence *F *F very approximately (need sim4 alignment) *F ~1987-900 = 254926-256070, 163-865 = 256739-256036, 2568-1973 = 254288-254883, *F 164-68 = 256797-256893, 71-1 = 257060-257130 *F *F > Crlbp *F S65395(11aa) by Pattern Search against predicted proteins at BDGP *F =FBan0001668=CG1668|FBan0012467|CT4586=Pbprp2=FBgn0011280 *F *F > ect *F A61047(280aa) by BLASTP against predicted proteins at BDGP *F =CG11965|FBan0011965|CT37129|FBan0011965 last_updated:000321(429aa) *F *F Score = 168 (59.1 bits), Expect = 5.1e-19, Sum P(2) = 5.1e-19 *F Identities = 38/50 (76%), Positives = 38/50 (76%) *F *F Query: 1 MKFVIXXXXXXXXXXQIEASPLQRLSRSERAVSPQQTVNNEVAPAVRPAS 50 *F MKFVI QIEASPLQRLSRSERAVS QQTVNNEVAPAV PAS *F Sbjct: 1 MKFVILLCVLSALLLQIEASPLQRLSRSERAVSRQQTVNNEVAPAVAPAS 50 *F *F Score = 75 (26.4 bits), Expect = 5.1e-19, Sum P(2) = 5.1e-19 *F Identities = 17/41 (41%), Positives = 17/41 (41%) *F *F Query: 155 LDEXXXXXXXXXXXGISDGVDLPAESXXXXXXXXXXXXXDE 195 *F LDE GISDGVDLPAES DE *F Sbjct: 155 LDEVAPAAASSVSAGISDGVDLPAESGNAAEVLAAGNAADE 195 *F *F > EG:34F3.2 - part of CG12467 *F O77432(504aa) by BLASTP against predicted proteins at BDGP *F =CG12467|FBan0012467|CT32681|FBan0012467 last_updated:000321(1376aa) *F *F 77-524 of CG12467 corresponds to 38-485 of EG:34F3.2 *F 40-47 of ' 1-8 ' *F *F > Gprk3 *F C41615(55aa) by BLASTP against predicted proteins at BDGP *F =CG8224|FBan0008224|CT8241|FBan0008224 last_updated:000321(601aa) *F *F Score = 268 (94.3 bits), Expect = 8.0e-24, P = 8.0e-24 *F Identities = 51/55 (92%), Positives = 54/55 (98%) *F *F Query: 1 VVYRDLKSKNILVKSNLSCAIGDLGLAVRHVEKNDSVDIPSTHRVGTKRYMAPEI 55 *F + +RDLKSKNILVKSNLSCAIGDLGLAVRHVEKNDSVDIPSTHRVGTKRYMAPE+ *F Sbjct: 427 IAHRDLKSKNILVKSNLSCAIGDLGLAVRHVEKNDSVDIPSTHRVGTKRYMAPEV 481 *F *F > Gprk4 *F D41615(49aa) by BLASTP against predicted proteins at BDGP *F =CG3051|FBan0003051|CT10258|FBan0003051 last_updated:000321(582aa) *F Score = 222 (78.1 bits), Expect = 7.1e-19, P = 7.1e-19 *F Identities = 43/50 (86%), Positives = 46/50 (92%) *F *F Query: 1 VVYRDLKPKNLLLDHNMHAKIADFGLSNMMLDGEFLRTSA-SPNYMAPEV 49 *F +V+RDLKP+NLLLDHNMH KIADFGLSNMMLDGEFLRTS SPNY APEV *F Sbjct: 147 IVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEV 196 *F *F > GstD21 *F D46681(214aa) by BLASTP against predicted proteins at BDGP *F =CG4181|FBan0004181|CT13804|FBan0004181 last_updated:000321(215aa) *F *F 214/214 (100%) id *F *F > GstD23 *F E46681(214aa) by BLASTP against predicted proteins at BDGP *F =CG11512|FBan0011512|CT36385|FBan0011512 last_updated:000321(215aa) *F *F 213/214 (99%) id *F *F > GstD24 *F C46681(215aa) by BLASTP against predicted proteins at BDGP *F =CG12242|FBan0012242|CT13898|FBan0012242 last_updated:000321(210aa) *F *F 209/215 (97%) id (gap of 6aa in subject) *F *F > GstD25 *F B46681(214aa) by BLASTP against predicted proteins at BDGP *F =CG4423|FBan0004423|CT13946|FBan0004423 last_updated:000321(215aa) *F *F 214/214 (100%) id *F *F > GstD26 *F H46681(170aa) by BLASTP against predicted proteins at BDGP *F =CG4371|FBan0004371|CT13952|FBan0004371 last_updated:000321(224aa) *F *F 170/170 (100%) id *F *F > GstD27 *F JQ1378(212aa) by BLASTP against predicted proteins at BDGP *F =CG4421|FBan0004421|CT13954|FBan0004421 last_updated:000321(212aa) *F *F 211/212 (99%) id *F *F > Gta *F D46036(150aa) by BLASTP against predicted proteins at BDGP *F =CG4268|FBan0004268|CT13850|FBan0004268 last_updated:000321(952aa) *F *F Score = 705 (248.2 bits), Expect = 5.5e-71, P = 5.5e-71 *F Identities = 147/152 (96%), Positives = 147/152 (96%) *F *F Query: 1 VVYRAKDKRTNEIEALKRLKMEKEKEGFPITSRREINTLLKGGQHPNIVTVREIVVGSNM *F 60 *F VVYRAKDKRTNEI ALKRLKMEKEKEGFPITS REINTLLKG QHPNIVTVREIVVGSNM *F Sbjct: 571 VVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKG-QHPNIVTVREIVVGSNM *F 629 *F *F Query: 61 DKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQL-RAVAHLHDN-ILHRDLKTS *F 118 *F DKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQL RAVAHLHDN ILHRDLKTS *F Sbjct: 630 DKIFIVMDYVEHDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTS *F 689 *F *F Query: 119 NLLLSHKGILKVGDFGLAREYGSPIKK-TSLVV 150 *F NLLLSHKGILKVGDFGLAREYGSPIKK TSLVV *F Sbjct: 690 NLLLSHKGILKVGDFGLAREYGSPIKKYTSLVV 722 *F *F > Hmx *F Q24016(34aa) by BLASTP against predicted proteins at BDGP *F =CG5832|FBan0005832|CT18293|FBan0005832 last_updated:000321(263aa) *F *F Score = 169 (59.5 bits), Expect = 3.5e-14, P = 3.5e-14 *F Identities = 34/34 (100%), Positives = 34/34 (100%) *F *F Query: 1 FDLKRYLSSSERAGLAASLRLTETQVKIWFQNRR 34 *F FDLKRYLSSSERAGLAASLRLTETQVKIWFQNRR *F Sbjct: 158 FDLKRYLSSSERAGLAASLRLTETQVKIWFQNRR 191 *F *F > Hrb85CD - probably same gene as Hrb87F *F Q24486(386aa) by BLASTP against predicted proteins at BDGP *F =CG12749|FBan0012749|CT27250|FBan0012749 last_updated:000321(385aa) *F *F 198/198 (100%) id *F *F > ImpE3 *F A61046(331aa) by BLASTP against predicted proteins at BDGP *F =CG2723|FBan0002723|CT9257|FBan0002723 last_updated:000321(325aa) *F *F 188/191 (98%) id *F *F > l(2)rot - Cat 2 residue, BLASTN finds CGless seq *F S42089(542aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F X95246(2818bp) by BLASTN against all at BDGP *F =overlaps on opposite strand CG5504 *F CG5504|FBan0005504|CT17450|FBan0005504 last_updated:000321(1635bp) *F *F very approximately (need sim4 alignment) *F ~2632-1240, 1100-843 = 1-1365, 1378-1635bp of CG *F *F =gadfly|SEG:AE003461|gb|AE003461|Drosophila melanogaster genomic scaffold *F 142000013386038 section 10 of 15, complete sequence. *F *F 2818-1 = 228045-230862 of scaffold *F *F > l(3)73Ah *F PIR:JC4296(222aa) by BLASTP against predicted proteins at BDGP *F =CG4195|FBan0004195|CT13794|FBan0004195 last_updated:000321(222aa) *F *F 221/222 (99%) id *F *F > Lcp6 - Cat 1 residue, BLASTN finds CGless seq *F SPTREMBL:P92184(104aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F *F U84756(473bp) by BLASTN against all at BDGP *F =gb|AE003563|Drosophila melanogaster genomic scaffold 142000013386050 *F section 50 of 54, complete sequence. *F *F very approximately (need sim4 alignment) *F 330-13 = 242870-243193, poor but 462-334 = 27439-27567 *F *F > Lcp65Ab1 - Cat 2 residue, BLASTN finds CGless seq *F SPTREMBL:P92192(104aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F U84747(947bp) by BLASTN against all at BDGP *F =gb|AE003563|Drosophila melanogaster genomic scaffold 142000013386050 *F section 50 of 54, complete sequence. *F *F very approximately (need sim4 alignment) *F 946-1 = 242514-243462 *F *F > Lcp65Ab2 - Cat 2 residue, BLASTN finds CGless seq *F SPTREMBL:P92192(104aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F U84746(855bp) by BLASTN against all at BDGP *F =gb|AE003563|Drosophila melanogaster genomic scaffold 142000013386050 *F section 50 of 54, complete sequence. *F *F very approximately (need sim4 alignment) *F 855-1 = 245477-246316 *F *F > Mat89Ba *F SPTREMBL:Q27924(545aa) by BLASTP against predicted proteins at BDGP *F =CG6814|FBan0006814|CT21143|FBan0006814 last_updated:000321(689aa) *F *F CG has extra 27aa at N-term, a few polymorphisms *F *F > Mst40 - possibly untranslated? (Aubrey Apr 4) *F SPTREMBL:Q24437(23aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F Z22588(1387bp) by BLASTN against all at BDGP *F =gb|AE003466|Drosophila melanogaster genomic scaffold 142000013386038 *F section 15 of 15, complete sequence. *F *F very approximately (need sim4 alignment) *F 1-1387 = 161926-163314 *F 1-799 = 163309-164114, ~800-1387 = 164130-164722 *F (two closely related genes near each other) *F *F > NaCP37B - Cat 1 residue *F SPTREMBL:Q24308(113aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F X84408(384bp) by BLASTN against all at BDGP *F =CG9071|FBan0009071|CT24831|FBan0009071 last_updated:000321(7375bp) *F *F very approximately (need sim4 alignment) *F 1-240 = 6438-6677 of CG, 241-377 = 6700-6823 of CG *F *F > ph-d *F PIR:S23632(1589aa) by BLASTP against predicted proteins at BDGP *F =CG3895|FBan0003895|CT12875|FBan0003895 last_updated:000321(1211aa) *F from 130-594 of CG3895, CG missing exon, then a few other polymorphisms *F till end of CG, then *F =CG18414|FBan0018414|CT41888|FBan0018414 last_updated:000321(290aa) *F from 1300 to 1589 of S23632 for all of CG *F *F > Pk1 *F SPTREMBL:Q24057(36aa) by BLASTP against predicted proteins at BDGP *F =no proteins in GadFly *F U23827(110bp) by BLASTN against all at BDGP *F =gb|AE002760|Drosophila melanogaster genomic scaffold 142000013386034, complete sequence. *F *F 1-110 = 24362-24471 *F *F >PpD19 *F SPTREMBL:Q26247(24aa) by BLASTP against predicted proteins at BDGP *F =CG10930|FBan0010930|CT30615|FBan0010930 last_updated:000321(314aa) *F *F 24/24 (100%) id *F *F > PpD3 *F PIR:AAB22463(25aa) by BLASTP against predicted proteins at BDGP *F =CG8402|FBan0008402|CT24679|FBan0008402 last_updated:000321 *F *F 25/25 (100%) id *F *F > PpD33 *F PIR:AAB22469(24aa) by BLASTP against predicted proteins at BDGP *F =CG9842|FBan0009842|CT27780|FBan0009842 last_updated:000321(570aa) *F *F 24/24 (100%) id *F *F > PpD5 *F PIR:AAB22464(24aa) by BLASTP against predicted proteins at BDGP *F =CG10138|FBan0010138|CT10817|FBan0010138 last_updated:000321(346aa) *F *F 24/24 (100%) id *F *F > PpD6 *F PIR:AAB22465 (24aa) by BLASTP against predicted proteins at BDGP *F =CG8822|FBan0008822|CT25390|FBan0008822 last_updated:000321(336aa) *F *F 24/24 (100%) id *F *F >prd3 *F PIR:AAA28839(38aa) by BLASTP against predicted proteins at BDGP *F =no protein in GadFly *F M14551(114bp) by BLASTN against all at BDGP *F =CG10037|FBan0010037|CT28091|FBan0010037 last_updated:000321(2556bp) *F *F 1-114 = 2122-2235 *F *F > Rbp10 *F PIR:I48110(44aa) by BLASTP against predicted proteins at BDGP *F =CG3151|FBan0003151|CT38165|FBan0003151 last_updated:000321(68aa) *F =CG3151|FBan0003151|CT10570|FBan0003151 last_updated:000321(673aa) *F *F 43/43 (100%) id, 114-156 of CG *F *F > Rbp11 *F PIR:A47752(39aa) by BLASTP against predicted proteins at BDGP *F =no protein in GadFly *F *F closest match: *F CG17136|FBan0017136|CT38058|FBan0017136 last_updated:000321 *F Length = 135 *F *F Score = 152 (53.5 bits), Expect = 2.2e-12, P = 2.2e-12 *F Identities = 29/39 (74%), Positives = 30/39 (76%) *F *F Query: 1 FVGNLAPRRSKPRDRSAFAKYGPLRNVWVARNPPGFAFV 39 *F +VGNL SK AFAKYGPLRNVWVARNPPGFAFV *F Sbjct: 14 YVGNLGSSASKHEIEGAFAKYGPLRNVWVARNPPGFAFV 52 *F *F S51740(117bp) by BLASTN agianst all at BDGP *F =CG17136|FBan0017136|CT38058|FBan0017136 last_updated:000321(511bp) *F *F Plus Strand HSPs: *F *F Score = 490 (73.5 bits), Expect = 2.3e-16, P = 2.3e-16 *F Identities = 110/117 (94%), Positives = 110/117 (94%), Strand = Plus / Plus *F *F Query: 1 TTCGTGGGGAACCTGG-CTCCTCGGCGCTCCAAGCCACGAGATAGAAG-CGCATTTGCCA 58 *F T CGTGGG AACCTGG CTCCTCGGCG TCCAAGC ACGAGATAGAAG CGCATTTGCCA *F Sbjct: 93 TACGTGGGAAACCTGGGCTCCTCGGCG-TCCAAGC-ACGAGATAGAAGGCGCATTTGCCA 150 *F *F Query: 59 AATATGGACCCCTGCGAAACGTGTGGGTGGCCCGCAATCCACCAGGGTTCGCTTTCGTC 117 *F AATATGGACCCCTGCGAAACGTGTGGGTGGCCCGCAATCCACCAGG TTCGC TT GTC *F Sbjct: 151 AATATGGACCCCTGCGAAACGTGTGGGTGGCCCGCAATCCACCAGGTTTCGCCTTTGTC 209 *F *F > Rbp12 *F PIR:B47752(41aa) by BLASTP against predicted proteins at BDGP *F =CG5422|FBan0005422|CT17178|FBan0005422 last_updated:000321(464aa) *F =CG5422|FBan0005422|CT17194|FBan0005422 last_updated:000321(464aa) *F *F 39/41 (95%) id *F *F > Rbp2 *F PIR:B48110(43aa) by BLASTP against predicted proteins at BDGP *F =CG4429|FBan0004429|CT14414|FBan0004429 last_updated:000321(325aa) *F *F 40/43 (93%) id, 32-74 of CG *F *F > Rbp3 *F PIR:C48110(44aa) by BLASTP against predicted proteins at BDGP *F =CG17791|FBan0017791|CT39414|FBan0017791 last_updated:000321(378aa) *F *F 31/32 (96%) id, 149-180 of CG *F *F > Rbp5 *F PIR:E48110(48aa) by BLASTP against predicted proteins at BDGP *F =no protein in GadFly *F S51706(144bp) by BLASTN against all at BDGP *F overlaps opposite strand of *F CG3373|FBan0003373|CT11347|FBan0003373 last_updated:000321(2291bp) *F *F Minus Strand HSPs: *F *F Score = 635 (95.3 bits), Expect = 2.6e-22, P = 2.6e-22 *F Identities = 135/141 (95%), Positives = 135/141 (95%), Strand = Minus / Plus *F *F Query: 143 ACGAAACCGAA-CCCACTACCAAGACGACAACTACTACAACCACGCCAAAGCCCACTACC 85 *F AC A CCGAA CCCACTACCAAGACGACAACTACTACAACCACGCCAAAGCCCACTACC *F Sbjct: 1470 ACCACGCCGAAACCCACTACCAAGACGACAACTACTACAACCACGCCAAAGCCCACTACC 1529 *F *F Query: 84 ACAACGACAACCAAGAAGCCAACGACCACTACCACCACTACGACAACCACGCCGAAGCCA 25 *F ACAACGACAACCAAGAAGCCAACGACCACTACCACCACTACGACAACCACGCCGAAGCCA *F Sbjct: 1530 ACAACGACAACCAAGAAGCCAACGACCACTACCACCACTACGACAACCACGCCGAAGCCA 1589 *F *F Query: 24 ACAACTACTTAGTCCGCCAACA 3 *F ACAACTACT AG CCGCC ACA *F Sbjct: 1590 ACAACTACTAAG-CCGCCGACA 1610 *F *F > Rbp6 *F PIR:F48110(44aa) by BLASTP against predicted proteins at BDGP *F =no protein in GadFly *F S51715(132bp) by BLASTN against all at BDGP *F =gb|AE003524|Drosophila melanogaster genomic scaffold 142000013386050 section *F 11 of 54, complete sequence. *F *F Plus Strand HSPs: *F *F Score = 430 (64.5 bits), Expect = 9.1e-13, P = 9.1e-13 *F Identities = 90/95 (94%), Positives = 90/95 (94%), Strand = Plus / Plus *F *F Query: 30 TCCAGAGAGCTTACGCGATTACTTCGGACGTTACGGTGATATCTCAGAGGCTATGGTCAT 89 *F | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 314913 TTCAGAGAGCTTACGCGATTACTTCGGACGTTACGGTGATATCTCAGAGGCTATGGTCAT 314972 *F *F Query: 90 GAAGGATCCCACGACGCGCAGATCCAGAGGTTTTG 124 *F ||||||||||||||||||||||||||| | ||| *F Sbjct: 314973 GAAGGATCCCACGACGCGCAGATCCAGGTGAGTTG 315007 *F *F > Rbp7 *F PIR:G48110(44aa) by BLASTP against predicted proteins at BDGP *F =CG10377|FBan0010377|CT29098|FBan0010377 last_updated:000321(421aa) *F *F 44/44 (100%) id, 10-53 of CG *F *F > Rbp8 *F PIR:H48110(36aa) by BLASTP against predicted proteins at BDGP *F =CG10851|FBan0010851|CT41998|FBan0010851 last_updated:000321(132aa) *F =CG10851|FBan0010851|CT30371|FBan0010851 last_updated:000321(329aa) *F *F 31/36 (86%) id *F *F > repo - part of CG8045 *F PIR:A54282(612aa) by BLASTP against predicted proteins at BDGP *F =CG8045|FBan0008045|CT24072|FBan0008045 last_updated:000321(612aa) *F *F > Rya-r76CD - misassigned to CG10844 *F PIR:B49131(508aa) by BLASTP against predicted proteins at BDGP *F =CG10844|FBan0010844|CT30357|FBan0010844 last_updated:000321(5107aa) *F *F C terminal 4599-5107aa of CG *F *F Z18536(1528bp) by BLASTN against all at BDGP *F =CG10844|FBan0010844|CT30357|FBan0010844 last_updated:000321(15606bp) *F *F 1-1528 = 13794-15325 # *U FBrf0129568 *a Bayraktaroglu *b L. *t 2000.8.7 *T personal communication to FlyBase *u *F Here are the nc3 category gene matches to CGs (whenever possible) *F or scaffolds. *F In a few instances, scaffold coordinates were included to indicate *F the extent of the annotation changes necessary; the coordinates *F should be treated as approximate. *F *F Leyla *F *F &kgr;B-Ras CG1669|FBan0001669|CT4492| *F &zgr;COP CG3948|FBan0003948|CT13124| *F 312 CG9166|FBan0009166|CT8251| *F Aats-trp CG9735|FBan0009735|CT27510| *F ACXA CG17176|FBan0017176|CT38108| *F ACXB CG17174|FBan0017174|CT38106| *F ACXC CG5983|FBan0005983|CT18649| *F ACXD CG5712|FBan0005712|CT6304| *F ACXE CG17178|FBan0017178|CT38110| *F alpha-catenin-related CG2987|FBan0002987|CT10097| *F anon-69Ag MERGE CG14124|FBan0014124|CT33722| + CG14123|FBan0014123|CT33721| *F anon-71Aa CG13466|FBan0013466|CT32830| *F anon-Ryu CG3066|FBan0003066|CT10270| *F arouser CG4276|FBan0004276|CT37920| and |CT13981| *F bc10 CG4867|FBan0004867|CT15653| *F BcDNA:GH02384 matches CG-less sequence on AE003666 No good BLASTP match *F *F BcDNA:GH05668 unresolved, to be fixed during reannotation. *F Third exon of CG3364|FBan0003364|CT11313| overlaps with BcDNA:GH09668, but *F the predicted CG3364 CDS falls within an intron of BcDNA:GH05668, so *F there is no CDS overlap. *F *F BcDNA:LD16071 CG10106|FBan0010106|CT7946| *F BcDNA:LD22218 matches CG-less sequence on AE003809 No good BLASTP match *F BcDNA:LD37196 CG7242|FBan0007242|CT22341| *F best CG6264|FBan0006264|CT19608| *F BG:DS00004.11 CG2358|FBan0002358|CT7848| *F *F BG:DS00180.5 MERGE/SPLIT *F CG17988|FBan0017988|CT40163| + CG8855|FBan0008855|CT25428| *F aa 650-766 of CG17988 = aa 166-282 of 734 aa BG:DS00180.5 *F aa 66-292 of 832 aa CG8855 = aa 394-620 of 734 aa BG:DS00180.5 *F (aa 431-832 of CG8855 correspond to BG:DS00180.12) *F (no aa correspondence found to first 650 aa of CG17988) *F *F BG:DS00180.7 part of CG16882 *F CG16882 to be SPLIT: *F aa 8-421 of BG:DS00180.7 correspond to aa 504-917 of CG16882|CT37470 CDS *F aa 8-421 of BG:DS00180.7 correspond to aa 622-1035 of CG16882|CT37468 CDS *F aa 1-448 of CG16882 correspond to 448 aa BG:DS00180.8 CDS *F *F BG:DS00810.1 matches CG-less sequence on AE003645 No good BLASTP match *F BG:DS00810.2 matches CG-less sequence on AE003645 No good BLASTP match *F BG:DS00929.15 matches CG-less sequence on AE003646 No good BLASTP match *F BG:DS00929.16 matches CG-less sequence on AE003645 and AE003646 No good BLASTP match *F BG:DS00929.7 matches CG-less sequence on AE003646 No good BLASTP match *F BG:DS01514.3 matches CG-less sequence on AE003642 No good BLASTP match *F BG:DS02740.18 matches CG-less sequence on AE003650 No good BLASTP match *F BG:DS02795.3 matches CG-less sequence on AE003650 No good BLASTP match *F BG:DS03192.3 matches CG-less sequence on AE003646 No good BLASTP match *F BG:DS03192.4 matches CG-less sequence on AE003646 No good BLASTP match *F BG:DS03792.2 matches CG-less sequence on AE003643 No good BLASTP match *F BG:DS05899.6 matches CG-less sequence on AE003642 No good BLASTP match *F BG:DS06874.7 matches CG-less sequence on AE003645 No good BLASTP match *F BG:DS07295.4 matches CG-less sequence on AE003646 No good BLASTP match *F BG:DS07721.1 matches CG-less sequence on AE003644 No good BLASTP match *F BG:DS08249.1 matches CG-less sequence on AE003641 No good BLASTP match *F BG:DS08249.4 matches CG-less sequence on AE003641 No good BLASTP match *F BG:DS08249.5 matches CG-less sequence on AE003641 No good BLASTP match *F BG:DS09219.1 matches CG-less sequence on AE003644 No good BLASTP match *F *F bon CG5206|FBan0005206|CT16645| *F Bzd CG13761|FBan0013761|CT33242| *F c11.1 CG12132|FBan0012132|CT7740| (truncated) *F c12.1 CG12135|FBan0012135|CT7760| *F c12.2 CG12149|FBan0012149|CT8343| (truncated) *F Ca-&bgr; CG6320|FBgn0032353|CT19694 (fix) *F *F Caps MERGE CG17881 (BcDNA:GH07283) + CG18026 *F CG17881|FBan0017881|CT39782| (aa 190-910 of Caps) *F CG18026|FBan0018026|CT40344| (aa 715-1282 of Caps) *F (these 2 have been annotated as overlapping transcripts: they *F share all or parts of 3 exons; structure to be fixed) *F *F cathD CG1548|FBan0001548|CT3996 *F Catsup CG10449|FBan0010449|CT29330 *F ced-6 CG11804|FBan00011804|CT33506 *F CHORD CG6198|FBan0006198|CT19440 *F chrw CG3870|FBan0003870|CT12899 *F cIF2 CG10840|FBan0010840|CT30355 *F cngl CG9176|FBan0009176|CT26220 *F COQ7 CG14437|FBan0014437|CT34099 *F *F Crg-1 No BLASTP match, no 'CT' matches, but multiple BLASTN matches *F to contigs, may contain repeat, to be checked at reannotation. *F *F Crtp CG13580|FBan0013580|CT32962| *F dbo CG6224|FBan0006224|CT19492| *F desert matches CG-less sequence on AE003554 No good BLASTP match *F Dip1 CG15367|FBan0015367|CT35387| *F Dip2 CG9771|FBan0009771|CT27623| *F Dip3 CG12767|FBan0012767|CT30557| *F disp CG2019|FBan0002019|CT6508| *F DSH3PX1 CG6757|FBan0006757|CT20989| *F dynactin-subunit-p25 CG10846|FBan0010846|CT30375| *F Ef1&ggr; CG11901|FBan0011901|CT5034| *F EG:140G11.3 CG4015|FBan0004015|CT41858| *F EG:96G10.8 CG14265|FBan0014265|CT33887| *F EG:BACR25B3.1 SPLIT CG7981|FBan0007981|CT23996| (corresponds to N-terminus) *F EG:BACR25B3.10 SPLIT CG7981|FBan0007981|CT23996| (corresponds to C-terminus) *F EG:BACR25B3.4 CG8310|FBan0008310|CT24372| *F Important: No CDS (translation) is available for this gene. *F EG:BACR25B3.9 CG8590|FBan0008590|CT24639| *F EG:BACR37P7.5 CG12470|FBan0012470|CT32706| *F EG:BACR43E12.4 CG3526|FBan0003526|CT11880| (extend N-terminus of CG) *F EG:BACR43E12.5 CG14418|FBan0014418|CT34075| *F EG:BACR43E12.6 CG14417|FBan0014417|CT34074| *F EG:BACR7A4.2 CG3713|FBan0003713|CT12463| *F EG:EG0007.5 matches CG-less sequence on AE003430 No good BLASTP match *F eIF-3p66 CG10161|FBan0010161|CT28571| *F *F epsin-like part of CG13854|FBan0013854|CT33368| + CG13853|FBan0013853|CT33367| *F MERGE/SPLIT (note: CG13854 is 1370 aa and only the first 448 aa *F of that matches the published epsin-like mRNA (CDS 642 aa). Rest *F of epsin-like matches the neighboring CG13853) *F *F eso18E CG14233|FBan0014233|CT33849| *F espinas CG12833|FBan0012833|CT31965| *F Fad2 CG7923|FBan0007923|CT23928| *F fbl CG5725|FBan0005725|CT37729| (aa 112-512)(1st exon of CG5725 wrong, *F coordinates should be 55698-56257 on Acc. AE003591) *F FKBP59-bp1 CG4535|FBan0004535|CT14666| *F fne CG4396|FBan0004396|CT7563| (CG missing 2 exons, last exon truncated) *F FU12 CG9233|FBan0009233|CT26378| *F G&ggr;30A CG18511|FBan0018511|CT42246| (intron-exon structure to be fixed, *F coding exons should be 72488..72600,91049..91154 of AE003624) *F GCR(ich) CG5812|FBan0005812|CT17997| *F Glu-RIB CG4481|FBan0004481|CT14586| *F glycerol-3-phosphate-acyltransferase CG4625|FBan0004625|CT14810| *F GNBP1 CG6895|FBan0006895|CT21350| *F GNBP2 (SPLIT?) CG4144|FBan0004144|CT13694| (most of published complete 461 aa CDS contained *F in the N-terminus of 1189 aa CG4144; CG4144 will probably have to be split.) *F GNBP3 CG5008|FBan0005008|CT16088| *F *F graal MERGE (3-way merge): CG4821 + CG18403 + CG4948 *F (Tequila) CG4821|FBan0004821|CT15447| aa 1102-1462 of gr= 31-391 of CG4821 (391 aa) *F CG18403|FBan0018403|CT40927| aa 759-1046 of gr = aa 1-288 of CG18403 (290 aa) *F CG4948|FBan0004948|CT15880| aa 1-216 of gr = aa 1-216 of CG4948 (516 aa) *F *F grau CG3282|FBan0003282|CT11021| *F (N terminus of CG3282 CDS (1064 aa) corresponds *F to published grau complete CDS (544 of 570 aa); CG3282 to be split.) *F Gug CG6964|FBan0006964|CT21511| (small intron-exon differences) *F Hex CG5443|FBan0005443|CT17278| (all 3 Hex CDSs in here) *F hgo CG4779|FBan0004779|CT15337| *F HGTX MERGE CG13475|FBan0013475|CT32840| + CG4745|FBan0004745|CT15114| *F (1st 139 aa of 238 aa CG4745|CT15114 correspond to the C terminus of HGTX *F (not clear what the last ~100 aa of CG4745 correspond to) *F *F hoip CG3949|FBan0003949|CT13103| *F icln CG4924|FBan0004924|CT15822| *F Ilk CG10504|FBan0010504|CT29478| *F Jafrac1 CG1633|FBan0001633|CT4414| *F Jafrac2 CG1274|FBan0001274|CT2376| *F joey CG15736|FBan0015736|CT35978| *F *F Kai-RIA CG18039|FBan0018039|CT13538 *F Important: No CDS (translation) is available for this gene. *F Currently annotated as: *F AE003731 complement(join(<126216..126492,126550..126634, *F 126690..126893,126955..127185,127242..>127405)) *F correct CDS is: AE003731 complement(join(125064..125234,125299..125448,125508..125620, *F 125676..125901,125958..126159,126216..126492,126550..126634,126690..126893,126955..127185, *F 127242..127405,127466..127858,127917..128139,128197..128357,128440..128548)) *F *F katanin-60 CG10229|FBan0010229|CT28759| *F katanin-80 MERGE CG13956|FBan0013956|CT33508| + CG9910|FBan0009910|CT27906| *F Ku80 CG13241|FBan0013241|CT32491| *F kurtz-arrestin CG1487|FBan0001487|CT3719|FBan0001487 *F lack CG4943|FBan0004943|CT15876| *F larp CG14066|FBan0014066|CT33635| *F LRP6 CG5912|FBan0005912|CT15575| (missing first 66 aa) *F mab-2 CG4746|FBan0004746|CT15175| *F *F MAP-kinase-phosphatase CG6238|FBan0006238|CT19498| (first 319 aa only, missing *F rest of 1045 aa protein because of intron-exon annotation *F problems) *F CDS annotation on AE003750 reads: *F complement(join(3645..3843,3907..3972,4265..4656, 4722..4967,5864..5920)) *F should be: *F complement(join(1248..1250,1319..3227,3313..3582,3649..3843,3907..3972,4265..4656, 4722..4967,5864..5920)) *F *F mary CG13399|FBan0013399|CT32745| *F Mdh CG5889|FBan0005889|CT18483| *F mei-217 matches CG-less sequence on AE003504 No good BLASTP match *F Men CG10120|FBan0010120|CT38328| *F Menl-1 CG7964|FBan0007964|CT23838| *F Menl-2 CG7969|FBan0007969|CT23844|F *F meso18E CG14233|FBan0014233|CT33849| *F mig CG8367|FBan0008367|CT24629|F *F miple2 CG18321|FBan18321|CT41577| *F Mkrn1 CG7184|FBan0007184|CT22183| *F ms(1)15 CG12157|FBan0012157|CT8493| *F MTF-1 CG3743|FBan0003743|CT12477| *F Myo28B1 CG6976|FBan0006976|CT21549| *F Myt1 CG10569|FBan0010569|CT29656| (CG longer at C terminus than published Myt1 CDS) *F nerfin-1 CG13906|FBan0013906|CT33443 *F NHP2 CG5258|FBan0005258|CT16795| *F *F nkd MERGE? CG18224|FBan0018224|CT41252| + CG11614|FBan0011614|CT33667 *F (The exons marked for CG11614|CT33667 belong to nkd, but the CDSs of *F nkd and CG11614 do not match because of frameshift. *F *F nkd coding exons on AE003518 are at coordinates join(155094..155157, *F 188492..188955,191296..192108, 192628..193004,193559..194627) *F CG18224|FBan0018224|CT41252| CDS coordinates are 188524..188964 *F CG11614|FBan0011614|CT33667 exon coordinates are *F join(<191274..192108,192628..193004,193559..194627)) *F *F nmdyn-D7 CG8362|FBan0008362|CT24641| (aa 156-387 of 387 aa CG matches nmdyn-D7 CDS) *F noe matches CG-less sequence on AE003524 No good BLASTP match *F NtR CG6698|FBan0006698|CT20794| *F Ogt CG10392|FBan0010392|CT9123| *F onecut CG1922|FBan0001922|CT5912| *F orbit matches CG-less sequence on AE003593 No good BLASTP match *F p115 CG1422|FBan0001422|CT3352| *F *F Pap CG9854|FBan0009854|CT27790| (CG longer at the C terminus than published CDS) *F PpD14 CG1455|FBan0001455|CT3539| (note: PpD14 is a 73 bp PCR fragment) *F *F pins CG5692|FBan0005692|CT17944| *F pontin CG4003|FBan0004003|CT13291| *F POSH CG4909|FBan0004909|CT15776| *F PQBP-1 matches CG-less sequence on AE003678 No good BLASTP match *F Pros&bgr;5 CG12323|FBan0012323|CT22275| *F ptr CG2841|FBan0002841|CT9712| *F Rad CG5692|FBan0005692|CT17944| *F ran CG1404|FBan0001404|CT3258| *F raw MERGE CG9321|FBan0009321|CT26527| + CG12437|FBan0012437|CT32326| *F RecQ4 CG7487|FBan0007487|CT22997| *F REG CG1591|FBan0001591|CT4191|F *F reptin CG9750|FBan0009750|CT27557| *F retinin (SPLIT?) CG13057|FBan0013057|CT32276| (retinin annotated as *F complete CDS corresponds to aa 395-585 of 585 aa CG13057) *F *F Rgl CG8865|FBan0008865|CT25420| (intron-exon boundaries to be fixed) *F *F ROC2 CG16988|FBan0016988|CT37703| *F sbb CG5580|FBan0005580|CT17646| *F sbr CG1664|FBan0001664|CT4634|F *F SCAMP CG9195|FBan0009195|CT26276| *F scro CG18452|FBan0018452|CT38833| corresponds to most of exons 2 and 3 *F of scro (intron-exon boundaries to be fixed) *F aligns with AE003199 *F scylla CG7590|FBan0007590|CT23193| *F sds22 CG5851|FBan0005851|CT18355| *F secretory-granule-neuroendocrine-protein CG1168|FBan0001168|CT2051| (CG longer by 10 aa *F at N terminus than published CDS) *F *F sens CG10714|FBan0010714|CT30029 on AE003538 but transcript structure is incorrect *F CDS on AE003538 annotated as: join(47970..48089,48411..48506) *F Should be: join(47970..48129,48411..49492,51423..51573,51810..51927,52611..52724) *F *F serpin-27A CG11331|FBan0011331|CT31629| *F SF1 CG5836|FBan0005836|CT18309| (missing one section and too short) *F SF2 CG6987|FBan0006987|CT21609| *F sip1 CG7238|FBan0007238|CT22315| *F SMC1 CG6057|FBan0006057|CT18959| *F Sox50E CG8404|FBan0008404|CT24693| (1st and last exons to be fixed, aa 19-688 *F match published CDS) *F SoxN CG18024|FBan0018024|CT37434| (1st aa to be fixed) *F SP1029 CG11956|FBan0011956|CT39158| *F span CG12352|FBan0012352|CT23594| by nucleotide match only *F Important: No CDS (translation) is available for CG12352 in GadFly or GenBank. *F *F sp2 CG8137|FBan0008137|CT24318| (structure to be fixed: missing internal *F exon and truncated early) *F *F SP295 (SPLIT?)CG11326|FBan0011326|CT31613| aa 65-391 of 419 aa SP295 CDS match aa *F 1-355 of 1024 aa CG11326 (w/ gap) *F sp3 CG9334|FBan0009334| (extend CG at N-terminus) *F sp4 CG9453|FBan0009453|CT26788| *F sp5 CG18525|FBan0018525|CT42292| *F SP460 matches CG-less sequence on AE003576 No good BLASTP match *F SP512 CG1200|FBan0001200|CT2130| *F SP555 CG14041|FBan0014041|CT33600| *F SP558 CG1155|FBan0001155|CT2019| *F spag CG13570|FBan0013570|CT32952| *F Spt5 CG7626|FBan0007626|CT23221| *F Sr-CII CG8856|FBan0008856|CT25430| (intron-exon boundaries to be fixed) *F Sr-CIII matches CG-less sequence on AE003577 No good BLASTP match *F adjacent to Sr-CI (see accession AF221506) *F Sras CG4852|FBan0004852|CT15587| *F stich1 CG17100|FBan0017100|CT34464| *F Sucb CG10622|FBan0010622|CT29754| *F *F Sulf1 CG6725|FBan0006725|CT20881| (Sulf1 accession is an incomplete CDS) *F tantalus CG6586|FBan0006586|CT20496| (extend CG at N-terminus) *F tara CG6889|FBan0006889|CT19738| *F thioredoxin CG3864|FBan0003864|CT12877| *F *F TO42 matches CG-less sequence on AE002799 and AE003122 *F (1st 335 bp match CG-less sequence on AE002799, bp 643-1288 match AE003122 *F and overlap with CG17452|FBan0017452|CT34185| which is on the opposite strand *F (do NOT merge with CG17452)). *F *F Trio CG9208|FBan0009208|CT8909| *F Tunen CG8805|FBan0008805|CT3034| (CG missing N-terminus) *F unknown-telomeric-protein-gene matches CG-less sequence on AE003163 No good BLASTP match *F vanin-like CG3648|FBan0003648|CT12241| (C-terminus to be fixed) *F vav CG7893|FBan0007893|CT23780| *F veg CG6657|FBan0006657|CT20658| *F ventrally-expressed-protein-D matches CG-less sequence on AE003457, No good BLASTP match *F vhl CG13221|FBan0013221|CT32465| *F yip2 CG4600|FBan0004600|CT14747| *F yip3 CG13549|FBan0013549|CT32924| *F yip6 CG17489|FBan0017489|CT38667| (yip6 partial CDS) *F yip7 CG6457|FBan0006457|CT19127| (yip7 partial CDS) *F Zyx102EF matches CG-less sequence on AE003846, No good BLASTP match # *U FBrf0129569 *a Misra *b S. *t 2000.8.9 *T personal communication to FlyBase *u *F nc5: genes with no SWP/TREMBL/PIR entry or protein_id but which look *F like STSs to me (807). Spectacularly boring but presumably easy. *F The large majority are P-element STSs. *F *F First installment, 8/9/00 *F ---------- *F *F >Aats-gln AQ034065 insertion at base 569 in the 603bp *F =l(3)05461 by BLASTN analysis by Joe against GadFly *F =CG10506|FBan0010506|CT29352|FBan0010506 last_updated:000321(2327bp) *F *F (forward orientation at nt 50 of transcript) *F *F >Aats-ile AQ026160 insertion at base 596 in the 603 bp *F =l(3)00827 by BLASTN analysis by Joe against GadFly *F =CG11471|FBan0011471|CT36277|FBan0011471 last_updated:000321(3317bp) *F =CG11471|FBan0011471|CT36261|FBan0011471 last_updated:000321(3278bp) *F =CG11471|FBan0011471|CT36257|FBan0011471 last_updated:000321(3366bp) *F *F (reverse orientation at nt 30 of transcript) *F *F >Aats-thr AQ034143 insertion at base 503 in the 764 bp *F =l(2)k04203 by BLASTN analysis by Joe against GadFly and at BDGP BLAST *F =CG5353|FBan0005353|CT37701|FBan0005353 last_updated:000321(2345bp) *F *F (reverse orientation at nt 928 of GadFly gene region in first intron) *F *F >Aats-val AQ026133 insertion at base 001 in the 440 bp *F =l(2)rI255 by BLASTN analysis by Joe against GadFly *F =CG4062|FBan0004062|CT13504|FBan0004062 last_updated:000321(3224bp) *F *F (forward orientation 22bp upstream of transcript) *F *F >Acap AQ034171 insertion at base 237 in the 877 bp *F =l(2)k00619 by BLASTN analysis by Joe against GadFly *F =CG4775|FBan0004775|CT15363|FBan0004775 last_updated:000321(992bp)? *F *F (reverse orientation 75bp upstream of transcript; CG5118 starts 605bp *F upstream on opposite strand, 530bp from insert) *F *F >Acon AQ034137 insertion at base 294 in the 579bp *F =l(2)k02301 by BLASTN analysis by Joe against GadFly *F =CG9244|FBan0009244|CT5286|FBan0009244 last_updated:000321(2676bp) *F *F (forward orientation 18bp upstream of transcript) *F *F >Adk2-44B AQ026101 insertion at base 032 in the 39 bp *F =l(2)k16120 by BLASTN analysis by Joe against GadFly and BLAST at BDGP *F =CG3140|FBan0003140|CT10496|FBan0003140 last_updated:000321(1072bp)? *F (reverse orientation at nt 177 of transcript) *F Doesn't make sense since CG3140 at 60B1--mistake *F *F no matches to genomic sequence except at 60B1 *F *F >Adk2-60B AQ025735 insertion at base 057 in the 64 bp *F =l(2)k04201 by BLASTN analysis by Joe against GadFly *F =CG3140|FBan0003140|CT10496|FBan0003140 last_updated:000321(1072bp) *F *F (reverse orientation at nt 72 of transcript) *F *F >Aly AQ026178 insertion at base 053 in the 60 bp *F =l(3)02267 by BLASTN analysis by Joe against GadFly *F =CG1101|FBan0001101|CT1611|FBan0001101 last_updated:000321(1321bp) *F *F (reverse orienatation at nt 5 of transcript) *F *F >Bka AQ025597 insertion at base 190 in the 297 bp *F =l(2)02695 by BLASTN analysis by Joe against GadFly *F =CG4539|FBan0004539|CT14682|FBan0004539 last_updated:000321(672bp) *F *F (forward orientation at nt 337 of transcript) *F *F >cap AQ026423 insertion at base 119 in the 126 bp *F =ms(3)00940 by BLASTN analysis by Joe against GadFly *F =CG9748|FBan0009748|CT27543|FBan0009748 last_updated:000321(3138bp) *F *F (forward orientation at nt 255 of transcript) *F *F >capt AQ034171 insertion at base 237 in the 877 bp *F =l(2)k00619 by BLASTN analysis by Joe against GadFly *F =CG4775|FBan0004775|CT15363|FBan0004775 last_updated:000321(992bp) *F *F (reverse orientation 75bp upstream of transcript; CG5061 starts 605bp *F upstreamon opposite strand, 530bp away from insert) *F *F >cbx AQ026413 insertion at base 142 in the 149 bp *F =ms(2)05704 by BLASTN analysis by Joe against GadFly *F =CG10536|FBan0010536|CT29563|FBan0010536 last_updated:000321(883bp)? *F *F (reverse orientation 46bp upstream of transcript; CG1675 starts 120bp *F downstream on opposite strand, 74bp away from insert) *F *F >CKI-related AQ073361 insertion probably at base 1 of 298bp *F =ms(3)04895 by BLASTN analysis by Joe against GadFly *F =CG6963|FBan0006963|CT21414|FBan0006963 last_updated:000321(3033bp) *F *F (forward orientation probably at 1106 of transcript) *F *F >Coprox AQ025946 insertion at base 067 in the 416 bp *F =l(2)k11018 by BLASTN analysis by Joe against GadFly *F =CG3433|FBan0003433|CT11567|FBan0003433 last_updated:000321(1358bp) *F *F (reverse orientation at nt 58 of transcript) *F *F >dev AQ026309 insertion at base 001 in the 149 bp *F =l(3)j2E11 by BLASTN analysis by Joe against GadFly *F =CG6833|FBan0006833|CT21153|FBan0006833 last_updated:000321(980bp) *F *F (reverse orientation at nt 75 of transcript) *F *F >Dmn AQ026100 insertion at base 345 in the 352 bp *F =l(2)k16109 by BLASTN analysis by Joe against GadFly *F =CG8269|FBan0008269|CT7980|FBan0008269 last_updated:000321(1336bp) *F *F (reverse orientation at nt 10 of transcript) *F *F >DNAprim AQ026282 insertion at base 223 in the 723 bp *F =l(3)j10B2 by BLASTN analysis at BDGP against GadFly *F =CG5553|FBan0005553|CT17562|FBan0005553 last_updated:000321(1606bp) *F *F (reverse orientation at nt 22 of transcript) *F *F >domino AQ034011 insertion at base 377 in the 416 bp *F =l(2)k08108 by BLASTN analysis at BDGP against all *F =CG9696|FBan0009696|CT27330|FBan0009696 last_updated:000321(10116bp) *F *F (forward orientation at nt 589 of transcript) *F *F >Dot AQ025853 insertion at base 093 in the 100 bp *F =l(2)k08012 by BLASTN analysis at BDGP against all *F =no annotations or genomic sequence in GadFly *F *F >dpld AQ025871 insertion at base 001 in the 167 bp *F =l(2)k08815 by BLASTN analysis at BDGP against all *F =CG1624|FBan0001624|CT4340|FBan0001624 last_updated:000321 *F *F (forward orientation at nt 5 of transcript) *F *F >EfTuM AQ026265 insertion at base 016 in the 149 bp *F =l(3)L4569 by BLASTN analysis by Joe against GadFly *F =CG6050|FBan0006050|CT18977|FBan0006050 last_updated:000321(1576bp) *F *F (forward orientation at nt 488 of transcript) *F *F >eIF-3p40 AQ025877 insertion at base 112 in the 119bp *F =l(2)k09003 by BLASTN analysis by Joe against GadFly *F =CG9124|FBan0009124|CT26154|FBan0009124 last_updated:000321(1161bp) *F *F (reverse orientation at nt 51 of transcript) *F *F >Fatp AQ034026 insertion at base 137 in the 211 bp *F =l(2)k10307 by BLASTN analysis by Joe against GadFly *F =CG7400|FBan0007400|CT22721|FBan0007400 last_updated:000321(2681bp) *F *F (forward orienation at nt 448 of transcript) *F *F >fs(2)ltoPP43 AQ025856 insertion at base 078 in the 85 bp *F =l(2)k08115 by BLASTN analysis by Joe against GadFly *F =CG10528|FBan0010528|CT37301|FBan0010528 last_updated:000321(1216bp) *F *F (forward orientation at nt 92 of transcript) *F *F >Hmgs AQ025645 insertion at base 029 in the 151 bp *F =l(2)06214 by BLASTN analysis by Joe against GadFly *F =CG16796|FBan0016796|CT37345|FBan0016796 last_updated:000321(1939bp) *F *F (forward orientation at nt 60 of transcript) *F *F >Hsp60B AQ026417 insertion at base 320 in the 327 bp *F =ms(2)06619 by BLASTN analysis by Joe against GadFly *F =CG2830|FBan0002830|CT9652|FBan0002830 last_updated:000321(2043bp) *F *F (forward orientation at of nt 77 of transcript) *F *F >l(2)02637 AQ025596 insertion at base 162 in the 259 bp *F =l(2)02637 by BLASTN analysis by Joe against GadFly *F =CG10109|FBan0010109|CT28455|FBan0010109 last_updated:000321(1689bp) *F *F (reverse orientation at nt 171 of transcript) *F *F >l(2)03221 AQ025607 insertion at base 144 in the 151 bp *F =l(2)03221 by BLASTN analysis by Joe against GadFly *F =CG17753|FBan0017753|CT11457|FBan0017753 last_updated:000321(1226bp) *F *F (reverse orientation 78bp upstream of nt 14 in transcript, in first *F intron?; CG11866 upstream ends 160bp from 3' end of gene, CG11867 *F downstream starts 4.9kb away) *F *F >l(2)04227 AQ025624 insertion at base 222 in the 229 bp *F =l(2)04227 by BLASTN analysis by Joe against GadFly *F =CG8938|FBan0008938|CT25660|FBan0008938 last_updated:000321(1416bp)? *F *F (reverse orientation 124bp upstream of transcript; CG15613 downstream *F starts 5439bp away from start of CG8938, 5315bp away from insertion) *F *F >l(2)04431 AQ073281 insertion probably at base 4 in the 159bp *F =l(2)04431 by BLASTN analysis by Joe against GadFly *F =CG6392|FBan0006392|CT19928|FBan0006392 last_updated:000321(5868bp) *F *F (reverse orientation probably at nt 5592 of transcript) *F *F >l(2)05091 AQ025631 insertion probably at base 063 in the 475 bp *F =l(2)05091 by BLASTN analysis by Joe against GadFly *F =CG8946|FBan0008946|CT25678|FBan0008946 last_updated:000321(1782bp) *F =CG8946|FBan0008946|CT25696|FBan0008946 last_updated:000321(1746bp) *F *F (forward orientation at nt 271 of CT25678, 235 of CT25696) *F *F >l(2)05337 AQ025634 insertion probably at base 033 in the 40 bp *F =l(2)05337 by BLASTN analysis by Joe against GadFly *F =CG9239|FBan0009239|CT26270|FBan0009239 last_updated:000321(5404bp) *F *F (reverse orientation at nt 169 of transcript) *F *F >l(2)05510 AQ025639 insertion probably at base 611 in the 618 bp *F =l(2)05510 by BLASTN analysis by Joe against GadFly *F =CG13432|FBan0013432|CT32789|FBan0013432 last_updated:000321(1355bp) *F *F (forward orientation 371bp downstream of nt 80 of transcript, in first intron) *F *F >Mrp17 AQ034077 insertion at base 376 in the 869 bp *F =l(3)10534 using Joe's BLASTN analysis of GadFly and BLASTN at BDGP *F =CG5012|FBan0005012|CT16104|FBan0005012 last_updated:000321(642bp) *F *F (forward orientation 29bp upstream of transcript) *F *F >Nik AQ026296 insertion at base 098 in the 105 bp *F =l(3)j1E2 using Joe's BLASTN analysis of GadFly *F =CG16973|FBan0016973|CT37669|FBan0016973 last_updated:000321(4635bp) *F *F (reverse orientation at nt 5 of transcript) *F *F >Nnp-1 AQ034006 insertion at base 306 in the 531 bp *F =l(2)k07826 using Joe's BLASTN analysis of GadFly *F =CG12396|FBan0012396|CT26519|FBan0012396 last_updated:000321(2306bp) *F *F (forward orientation at nt 1 of transcript) *F *F >ox AQ026399 insertion at base 263 in the 270 bp *F =ms(2)00815 using Joe's BLASTN analysis of GadFly *F =CG8657|FBan0008657|CT25080|FBan0008657 last_updated:000321(1896bp)? *F *F (reverse orientation 143bp upstream of start of transcript; CG12373 ends *F 547bp upstream of transcript, 404bp away) *F *F >Pdsw AQ025920 insertion at base 014 in the 124 bp *F =l(2)k10101 using Joe's BLASTN analysis of GadFly *F =CG8844|FBan0008844|CT9259|FBan0008844 last_updated:000321(604bp) *F *F (forward orientation at nt 195 of transcript) *F *F >Pros26S-RS6A AQ026194 insertion at base 246 in the 253 bp *F =l(3)04210 using Joe's BLASTN analysis of GadFly *F =CG10370|FBan0010370|CT29122|FBan0010370 last_updated:000321(1773bp) *F *F (reverse orientation at nt 441 of transcript) *F *F >RFeSP AQ034029 insertion at base 019 in the 228 bp *F =l(2)k11704 using Joe's BLASTN analysis of GadFly *F =CG7361|FBan0007361|CT22681|FBan0007361 last_updated:000321(773bp) *F *F (reverse orientation nt 35 of transcript) *F *F >RpL30 AQ025911 insertion at base 197 in the 204 bp *F =l(2)k09918 using Joe's BLASTN analysis of GadFly *F =CG10652|FBan0010652|CT29824|FBan0010652 last_updated:000321(442bp)? *F *F (reverse orientation 120bp upstream of start of transcript; CG15171 is *F 1861bp away from CG10652 on opposite strand) *F *F >Rpp30 AQ034135 insertion at base 287 in the 624 bp *F =l(2)k01901 using Joe's BLASTN analysis of GadFly *F =CG11606|FBan0011606|CT33141|FBan0011606 last_updated:000321(925bp) *F *F (reverse orientation 26bp upstream of start of transcript) *F *F >Rs1 AQ025895 insertion at base 247 in the 318bp *F =l(2)k09514 using Joe's BLASTN analysis of GadFly *F =CG2173|FBan0002173|CT7100|FBan0002173 last_updated:000321(2389bp) *F *F (reverse orientation at nt 75 of transcript) *F *F >scat AQ026412 insertion at base 013 in the 505 bp *F =ms(2)05289 using Joe's BLASTN analysis of GadFly *F =CG3766|FBan0003766|CT12590|FBan0003766 last_updated:000321(3072bp) *F *F (reverse orientation at nt 1105 of transcript) *F *F >sdl AQ026431 insertion at base 028 in the 35 bp *F =ms(3)05090 using Joe's BLASTN analysis of GadFly *F =CG12363|FBan0012363|CT24202|FBan0012363 last_updated:000321(645bp) *F *F (reverse orientation at nt 58 of transcript) *F *F >sec13 AQ026165 insertion at base 052 in the 287 bp *F =l(3)01031 using Joe's BLASTN analysis of GadFly *F =CG6773|FBan0006773|CT21025|FBan0006773 last_updated:000321(1511bp) *F *F (forward orientation at nt 283 of trancript) *F *F >Sip1 AQ025652 insertion at base 184 in the 291bp *F =l(2)06373 using Joe's BLASTN analysis of GadFly *F =CG10939|FBan0010939|CT30645|FBan0010939 last_updated:000321(1697bp) *F *F (forward orientation, at nt 17 of transcript) *F *F >SrpR&bgr; AQ026363 insertion at base 351 in the 796 bp *F =l(3)rK561 using Joe's BLASTN analysis of GadFly *F =CG5950|FBan0005950|CT18686|FBan0005950 last_updated:000321(1557bp) *F *F (reverse orientation, around nt 832 of transcript) *F *F >Tom34 AQ073315 insertion at base 1 in the 426 bp? *F =l(2)03692 using Joe's BLASTN analysis of GadFly *F =CG2708|FBan0002708|CT9235|FBan0002708 last_updated:000321(3067bp) *F *F (forward orientation, probably at nt 27 of transcript) *F *F >Treh AF083508 632 bp *F =cDNA sequence using Joe's BLASTN analysis of GadFly *F =CG9364|FBan0009364|CT26593|FBan0009364 last_updated:000321(1892bp) *F =CG9364|FBan0009364|CT26591|FBan0009364 last_updated:000321(1948bp) *F =CG9364|FBan0009364|CT26595|FBan0009364 last_updated:000321(1813bp) *F *F >Vha36 AQ034158 insertion at base 354 in the 809 bp *F =l(2)k07207 using Joe's BLASTN analysis of GadFly *F =CG8186|FBan0008186|CT20335|FBan0008186 last_updated:000321(1054bp) *F *F (forward orientation at nt 59 of transcript) *F *F >Vha68-2 U59147 4405bp *F =cDNA sequence using Joe's BLASTN analysis of GadFly *F =CG3762|FBan0003762|CT37353|FBan0003762 last_updated:000321(2048bp) *F =CG3762|FBan0003762|CT12570|FBan0003762 last_updated:000321(2074bp) *F =CG3762|FBan0003762|CT37359|FBan0003762 last_updated:000321(2136bp) *F *F >Xbp1 AQ034031 insertion at base 585 in the 804bp *F =l(2)k13803 using Joe's BLASTN analysis of GadFly *F =CG9415|FBan0009415|CT26690|FBan0009415 last_updated:000321(2024bp) *F *F (reverse orientation at nt 88 of transcript) # *U FBrf0129570 *a Levis *b R. *t 2000.9.15 *T personal communication to FlyBase *u *F Personal communication from Robert Levis, 15 September 2000 *F *F Subject: synonym for P{wA[R]}4-12. *F *F P{w[+]}AR4-32 is almost certainly the same as P{wA[R]}4-12: *F *F O32(1AC) is the original designation for what was renamed the P{wA[R]}4-12 *F insertion, which we published as having an insertion site in 12BC. I think *F that the (1AC) just means that transformant O32 had more than one insert *F and that (1AC) was one of the single-insert transformants derived from it. # *U FBrf0129571 *a Beaton *b A. *t 2000.10.12 *T personal communication to FlyBase *u *F Personal communication to FlyBase from Amy Beaton *F *F Please add a second transposon for the BFD strain l(2)01482. The flank *F AQ025587 is from a second P at 43E11,16. A new slide was made, confirming *F the additional site. *F *F The original report lists one insertion at 29C. # *U FBrf0129572 *a Pimpinelli *b S. *c S. *d Pimpinelli *t 2000.10.10 *T personal communication to FlyBase *u *F Date: Tue, 3 Oct 2000 16:22:59 -0400 (EDT) *F From: Leyla Bayraktaroglu *F Subject: abnormal oocyte gene *F To: pimpinelli@axcasp.caspur.it *F Cc: leyla@morgan.harvard.edu *F *F Dear Dr. Pimpinelli, *F *F I am a molecular curator at FlyBase. We are trying to match known *F genes to genes predicted from genome project/Celera sequence. I am *F writing to find out if you can provide us with any sequence *F information on the abnormal oocyte (abo) gene. *F *F The only abo sequence information we now have in FlyBase *F comes from the Lavorgna et al. Genetics 123 (3), 485-494 (1989) *F paper (accessions X17392, X17393). *F *F I wrote to Dr. Tomkiel, and he referred me to you. *F *F Any information you send will be curated as a personal communication *F from you to FlyBase. *F *F Thank you very much for your time, *F *F Leyla Bayraktaroglu *F Curator, FlyBase *F *F Date: Tue, 10 Oct 2000 14:08:56 +0200 *F From: Pimpinelli Sergio *F To: Leyla Bayraktaroglu *F Subject: Re: abnormal oocyte gene *F *F Dear Leyla *F Here attached is the abo DNA and protein sequences. We send a manuscript on *F abo molecular analysis were we describe these sequences. *F Best Regards *F Sergio Pimpinelli *F *F [Curator's note: author's formatting of the protein and DNA sequences *F was lost during conversion of the BinHex file.] *F *F 74 GAATTCTTTCATTTATAAATAACGTTTTTTTTTAATCTTGAAAAGCATTAATATT *F *F 148 ATTATTATTATTATACTTTTTTTTACAACAAAACTTTTGTAGACAGAGTATATTTTTGTAATCTAACTGCGGTCA *F *F 205 CACTTTACTTTAGGTACCTTCCGATCGGAAGAAGAACCCGACTGACATTAGGAATAGGAACTTGGCAGAACTTTC *F *F 280 CCAGGATCTGTCAGAATGGCCTACAAGAAGCGCCTACAATCGCAGAACCTCGTGCATCTGCTGCAGAACCGCGAG *F 1 M A Y K K R L Q S Q N L V H L L Q N R E *F *F *F 355 TCCGGATACACCAACGTGGGCCAACAGCCGGGCAGGATCGGCTGTTCTCGGTACGAGCGTCTCTTCTACAAGTGC *F 21 S G Y T N V G Q Q P G R I G C S R Y E R L F Y K C *F *F *F 430 ATCACGCCTTGCCTGACAATTGACTCGATTACCATTCCGCCGATCTACCTGCGCAAGTTTACGCCGGATCGGAGA *F 46 I T P C L T I D S I T I P P I Y L R K F T P D R R *F *F *F 505 AAACTGTTGGCCTTCTCGCAGGATCAGCGCAGCCTGCTCATATACAGCTACGGGGGTTCTAGTTGTGCGGCGGTG *F 71 K L L A F S Q D Q R S L L I Y S Y G G S S C A A V *F *F *F 580 GGTGAACTGATTCGCCAGGCGGATGTAGGCAGTGGCGAGTGCTTCAGTAGCCAGGACACCATACTCAAGAGCAGG *F 96 G E L I R Q A D V G S G E C F S S Q D T I L K S R *F *F *F 655 ATCTTTGAACGCCTGTTTCCCACAAAAGAGACGCTGAATCTATGCCAAGGCGACTTTGGCCTCTACTATCTGCAC *F 121 I F E R L F P T K E T L N L C Q G D F G L Y Y L H *F *F *F 730 CGGGAGTTCAGTGTGTTCCTGGAAGAGGGTCGTTATGCCATGCTGGCTGCCATGACCGTTGTGCGCGGCGCACTG *F 146 R E F S V F L E E G R Y A M L A A M T V V R G A L *F *F *F 805 CCGGTCGATGACTACGTACGGTACCCAGATCTGTTCGACAAAGTGGACGCCTTCTCGTATGTGTTCTTTCTGGTG *F 171 P V D D Y V R Y P D L F D K V D A F S Y V F F L V *F *F *F 880 GATTTGAAGCTAGGCGTCGTCACAGATAGACTTATCCTGCCTAACGACTCCATCGTCATTGCCCACAATCATGGA *F 196 D L K L G V V T D R L I L P N D S I V I A H N H G *F *F *F 955 ATTTCCGTGTTTGGCTCAACAGTGATGATGATGTCCCGGCTGCATCAGTGCGTTTACGTCTATTGGGTGAATGAC *F 221 I S V F G S T V M M M S R L H Q C V Y V Y W V N D *F *F *F 1030 GGTAAGTTCCACCAGCAGGAGACTATTGGTCCAGGCCGAGGGACTTTATCGAGAAGGCTACCACAGACTTTGACA *F 246 G K F H Q Q E T I G P G R G T L S R R L P Q T L T *F *F *F 1105 ATCCTTGACGCCACCACAGTTTTGCCCATAACGCACATTAAACAGCGCGTCCTTAGCTTTCTGTACCGTAAAATT *F 271 I L D A T T V L P I T H I K Q R V L S F L Y R K I *F *F *F 1180 AATGATAAAAGTGGCAACCCGACGGAAAGTCAGAAGTCGTTTTATAAAAACTTTGAGTATGTAAGTAACATTATA *F 296 N D K S G N P T E S Q K S F Y K N F E Y *F *F *F 1255 AAGAGTGACAACGGTCTTCACATCCTTACATATCTCATTTAGATAGAACACATGATCATGGAGAGAATGCATCTG *F 316 I E H M I M E R M H L *F *F *F 1330 GTGAACAATGAACTTCTAATGTTGCGCTATGAAGAGCGGCCAAATGGTACCGATACCATGCCCATGGCTACATCA *F 327 V N N E L L M L R Y E E R P N G T D T M P M A T S *F *F *F 1405 CCGCGCCGCTTGTATGTATTCTACACCATAACTGGCGAAGAAGTGGTTGGCGTTTATCCGGAATATTCCGTCAAC *F 352 P R R L Y V F Y T I T G E E V V G V Y P E Y S V N *F *F *F 1480 CTGCTGCAAATCTTACTGCAATTCAATGACTACATGAGCAATGATCGATCGCTGCAATTCGGTGATGCACCTTCA *F 377 L L Q I L L Q F N D Y M S N D R S L Q F G D A P S *F *F *F 1555 TTGCCCATGCATTTTTTCCTAAGGCATACCTTTGCGGATTCCAATGAATCGGTCTCCGTCGATCGACATACGGCC *F 402 L P M H F F L R H T F A D S N E S V S V D R H T A *F *F *F 1630 TTGCGCTTCAACCCCAGCGTTCCACTCAGCTCCCAGAGCCTTTCCTCGTCGCCCTATCTAAAGTTCAACGAATTT *F 427 L R F N P S V P L S S Q S L S S S P Y L K F N E F *F *F *F 1705 AGATACGACAGTCGGTACGTGTTCCCTCTGGAGCAACCCCATCGCTGCTGCAACGATCCTATTGTGTTTCTTGAC *F 452 R Y D S R Y V F P L E Q P H R C C N D P I V F L D *F *F *F 1780 GTGCCACGGATTCAATCAAGTTTAGACTGCACGCTACGGCCGCCGGCATTTAAATCCTTTGGCGCCACGGGAATT *F 477 V P R I Q S S L D C T L R P P A F K S F G A T G I *F *F *F 1855 GTGCGCCTTTATTTGGCATCCCTTTGACCCACTAGTGCTCAGTATCCAGAAATGCATGAACAGCTACGTCTACCA *F 502 V R L Y L A S L * *F *F 1930 CGTGCACTTGTATAACCACAGCACAATAGTGGAAAAGTAGACTGCCTAATATTTACATACATGATTTTGTACATT *F *F 2005 TGTGTATAAAACATATTCAAGTACCTTTTATATTATGAAATATATGTGATACTAAAATATCTCGTTACCTTTTCA *F *F 2080 GTAATGTCTTTATTTACAATGGTAAGAGAACATTTCTTTCTTTTATTTATTCAAAGACTGTGAAAATTGTGAAAA *F *F 2155 TTCCAAGTACTGTTTAAATCTTTAATTCGACTTTGCTACATTGTATTGAAGGCCGACTTGAAACTTGAGGCCCAA *F *F 2230 TCCGACGTTTAAAGTTTCATGTAACATTGTATCTCGACTGCGACTACGTTGAAGATTGGTGGTGACCGTCCTCGT *F *F 2274 TGTATTCCTTGTTGACCTTAAGGGAATGATTAATAACGCTCGAG # *U FBrf0129573 *a Kidd *b T. *c T. *d Kidd *t 2000.10.26 *T personal communication to FlyBase *u *F Subject: Re: sun (stunted) gene *F Date: Thu, 26 Oct 2000 22:02:28 -0700 *F From: Tom Kidd *F To: "Leyla Bayraktaroglu" *F *F Dear Leyla, *F *F Stunted is the Drosophila ATP synthase epsilon subunit and corresponds *F to CG9032. We have a DNA change supporting this, and also have phenotypic *F analysis. The maternal effect leads to abnormalities which are most *F apparent in the cortical embryonic divisions. The zygotic effect is that *F the first instar larvae grow very slowly and survive for a number of days *F before dying. *F *F A manuscript prepared jointly between the Ish-Horowicz and the Sullivan *F labs, and co-ordinated by myself and Robin Abu-Shumays is to be submitted *F very soon. *F *F Incidentally, the gene we initially thought was stunted is UBL3, a *F Drosophila homologue of HCG genes and is annotated as such - I include *F this in case you have access to my original personal communication to *F Flybase. *F *F Chadwick BP, Kidd T, Sgouros J, Ish-Horowicz D, Frischauf AM. *F Cloning, mapping and expression of UBL3, a novel ubiquitin-like gene. *F Gene. 1999 Jun 11;233(1-2):189-95. *F *F Regards, *F Tom Kidd *F *F > Date: Wed, 25 Oct 2000 16:55:24 -0400 (EDT) *F > From: Leyla Bayraktaroglu *F > Subject: sun (stunted) gene *F > To: tkidd@exelixis.com *F > *F > Dear Dr. Kidd, *F > *F > I am a molecular curator at FlyBase. One of our current goals *F > is to match "known" Drosophila genes to genes predicted from *F > Celera/BDGP sequence. I am writing to ask if you have *F > any further information on the "stunted" gene described in the *F > 1995 Drosophila conference abstract *F > Kidd, T., Katzen, A., Pinchin, S.M., Jimenez, G., Ish-Horowicz, D. *F > stunted locus is required for cellularization and larval growth. *F > A. Dros. Res. Conf. 36 1995 :163B *F > *F > Thanks very much for your help. *F > *F > Leyla Bayraktaroglu *F > Curator, FlyBase *F > # *U FBrf0129623 *a Degtyarenko *b K. *t 2000.9.19 *T personal communication to FlyBase *u *F From fbserver@ebi.ac.uk Tue Sep 19 11:53:25 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 19 Sep 2000 11:53:25 +0100 *F Date: Tue, 19 Sep 2000 11:52:53 +0100 (BST) *F From: fbmailer@bio.indiana.edu *F Reply-to: kirill@ebi.ac.uk *F To: flybase-help@morgan.harvard.edu *F Subject: mitochondrial P450s in Drosophila *F Content-Length: 790 *F *F comments: CYP12A4, CYP12A5, CYP12B2, CYP12C1, CYP12D1, CYP12E1, CYP301A1 *F and CYP302A1 have more similarity to Musca domestica CYP12A1, *F which is a mitochondrial protein, and to mammalian mitochondrial *F P450s than to microsomal P450s. Therefore for these P450s *F 'mitochondrial' is more plausible cellular location *F *F Source of information: *F 1) Rene Feyereisen (personal communication) *F 2) BLAST searches of the above proteins *F 3) Guzov, V.M., Unnithan, G.C., Chernogolov, A.A. and Feyereisen, R. *F (1998) CYP12A1, a mitochondrial cytochrome P450 from the house fly. *F Arch. Biochem. Biophys. 359, 231-240. *F mailto: flybase-help@morgan.harvard.edu *F realname: Kirill Degtyarenko *F reply-to: kirill@ebi.ac.uk *F *F Sent from computer iralell.dhcp.ebi.ac.uk # *U FBrf0131019 *a Klaembt *b C. *t 2000.8.16 *T personal communication to FlyBase *u *F Date: Wed, 16 Aug 2000 13:40:49 \+0100 *F To: Chihiro Yamada *F From: Christian Klaembt *F Subject: Re: FlyBase Query (cy729) *F >Dear Dr Klaembt, *F > *F >I sent you a Query a few weeks ago. I was wondering if you have had a *F >chance to look at it yet? I enclose the email for your convenience. *F >Sorry I had completely forgotten to answer your email. *F >Hummel et al, 2000, Neuron 26(2): 357-370. *F > *F >I have a few questions I was hoping you could answer for me. *F > *F .. *F >2. ftz-GAL4 and 44-GAL4 *F >======================= *F >You mention 2 GAL4 lines in your paper, ftz-GAL4 and 44-GAL4, that you *F >state as being unpublished. Could you provide me with a few details *F >about these lines? Are they enhancer trap or promoter fusion lines? *F >3. P0163 *F >======== *F >You mention a GAL4 line P0163. Can you tell me what transposon was *F >used in your screen to generate this line? *F all lines are enhancer trap lines generated with the PGAL4, white *F transposon (X-chr.) from Andrea Brand. *F We have checked by in situ hybridization to polytene chr. that ftz-GAL *F carries an insertion in the ftz region. *F i hope this answers your questions, *F best wishes christian> *F > *F >Any new information that you give to me will be curated as a personal *F >communication from you to FlyBase, if thats O.K. with you. *F > *F >Best wishes, *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph: 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- *F > *F > *F >----- End Included Message \----- *F Dr. Christian Klämbt *F Institut fuer Neurobiologie *F Universität Münster *F Badestrasse 9 *F D-48149 Münster Germany *F Phone xx49 251 83 2 1122 *F FAX xx49 251 83 2 4686 *F email klaembt@uni-muenster.de *F ==== *F From cy200@gen.cam.ac.uk Fri Jul 28 15:09:38 2000 *F To: klaembt@mail.uni-muenster.de *F Subject: FlyBase Query (cy729) *F From: Chihiro Yamada *F Date: Fri, 28 Jul 2000 15:15:44 \+0100 *F Dear Dr Klaembt, *F I am currently curating your paper for FlyBase: *F Hummel et al, 2000, Neuron 26(2): 357-370. *F I have a few questions I was hoping you could answer for me. *F 1. UAS-MAP2c *F ============ *F In fig.6 you mention a UAS-MAP2c line. there is only one such *F construct in our records, could you confirm that this the same *F construct as the one I describe below? If it is not, could you give me *F some information about this line please? Where did you get this line *F from? Do you have any of the molecular details of this construct? *F Rnor\MAP2Scer\UAS.cAa *F Adam, 1996.1.17, personal communication to FlyBase *F Warrick et al., 1999, Nature Genetics 23(4): 425--428 *F P{UAS-MAP2.A} *F UAS sequences direct the expression of @Rnor\MAP2@ c-transcript *F coding region. *F 2. ftz-GAL4 and 44-GAL4 *F ======================= *F You mention 2 GAL4 lines in your paper, ftz-GAL4 and 44-GAL4, that you *F state as being unpublished. Could you provide me with a few details *F about these lines? Are they enhancer trap or promoter fusion lines? *F If they are the former, could you tell me what transposon was used to *F generate the lines? If they are the latter could you give me some *F molecular details for these constructs? *F 3. P0163 *F ======== *F You mention a GAL4 line P0163. Can you tell me what transposon was *F used in your screen to generate this line? *F Any new information that you give to me will be curated as a personal *F communication from you to FlyBase, if thats O.K. with you. *F Best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0131025 *a Hiromi *b Y. *t 2000.8.17 *T personal communication to FlyBase *u *F From yhiromi@lab.nig.ac.jp Thu Aug 17 11:42:06 2000 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 11:42:06 \+0100 *F Mime-Version: 1.0 *F X-Mailer: Macintosh Eudora Pro Version 4.0.1Jr1 *F Date: Thu, 17 Aug 2000 19:48:30 \+0900 *F To: Chihiro Yamada *F From: Yasushi Hiromi *F Subject: Re: FlyBase Query (cy735b) *F Dear Dr. Yamada, *F As Dr. Lu states, I provided the B38 strain to him. *F Both B38 and H214 are enhancer trap insertions into the klingon gene (1). *F However, they are not the same stock; B38 has an insertion of the plwB *F element (2), whereas H214 (3) carries the HZ element (4). B38 was generated *F in an enhancer trap screen done in Corey Goodman's laboratory, and we *F (Samantha Butler and Yasushi Hiromi) identified that it is an insertion in *F the klingon gene. Although we have not published about B38, we are happy to *F share the stock and the information with the fly community. It can be *F referred to as 'S. Butler and Y. Hiromi, personal communication'. *F 1. Butler SJ, Ray S, Hiromi Y (1997). klingon, a novel member of the *F Drosophila immunoglobulin *F superfamily, is required for the development of the R7 photoreceptor neuron. *F Development 124(4):781-92. *F 2. The reference is either Genes Dev. 1989 Sep;3(9):1301-13 or Bioessays. *F 1990 May;12(5):199-204. *F 3. Mlodzik M, Hiromi Y, Goodman CS, Rubin GM (1992). The presumptive R7 *F cell of the developing Drosophila eye receives positional information *F independent of sevenless, boss and sina. Mech Dev 37(1-2):37-42. *F 4. Mlodzik M, Hiromi Y, Weber U, Goodman CS, Rubin GM (1990). The *F Drosophila seven-up gene, a member of the steroid receptor gene superfamily, *F controls photoreceptor cell fates. Cell 60(2):211-24. *F I hope the information is useful to you. *F Sincerely, *F Yash Hiromi *F At 10:28 \+0100 00/08/17, Chihiro Yamada wrote: *F > Dear Dr Hiromi, *F > *F > I am currently curating for FlyBase: *F > *F > Zhang et al., 2000, Mech. Dev 95(1,2): 113--122 *F > *F > Having recently corresponded with Dr Xiangyi Lu, he suggested that I *F > talk to you about one of the questions I had relating to the paper *F > above. This is the question I was hoping you could answer for me. *F > *F > \--- *F > *F > I wrote: *F > > B38-lacZ *F > > ======== *F > > In your Experimental Procedures section you mention B38-lacZ. You *F > > state that it is 'allelic to H214-lacZ'. I'm not sure what you mean by *F > > this. Are the B38-lacZ and H214-lacZ are caused by the same insertion? *F > > Are they two identical insertions? Are they both insertions of the *F > > same transposon? I'd appreciate any information you could give about *F > > the relationship between B38-lacZ and H214-lacZ that might help me sort *F > > this out. *F > *F > Dr Lu wrote: *F > B38-lacZ WAS FROM Y. YIROMI'S UNPUBLISHED STOCK. WHAT I HAVE CITED IN THE *F >PAPER *F > IS WANT YIROMI COMMUNICATED TO ME. I DON'T HAVE A COPY OF THE PAPER IN FRONT *F >OF *F > ME SINCE ALL MY PAPERS HAVE BEEN SHIPPED TO BIRMINGHAM WHERE I AM MOVING TO *F >THIS *F > MONTH. THE BEST PERSON TO ASK IS DR. YIROMI. *F > *F > \--- *F > *F > If you could find the time to answer this question I would greatly *F > appreciate it. *F > *F > Best wishes, *F > *F > Chihiro *F > *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: c.yamada@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph: 01223-333963 *F > UK. FAX: 01223-333992 *F > \---------------------------------------------------------------------- *F Yasushi Hiromi *F National Institute of Genetics *F 1111 Yata, Mishima *F Shizuoka 411-8540, JAPAN *F phone: \+81-559-81-6767 *F FAX: \+81-559-81-6768 *F e-mail: yhiromi@lab.nig.ac.jp # *U FBrf0131038 *a Irvine *b K. *t 2000.9.4 *T personal communication to FlyBase *u *F Date: Fri, 01 Sep 2000 16:08:41 \-0400 *F Subject: Re: FlyBase Query (cy755) *F From: Ken Irvine *F To: Chihiro Yamada *F Chihiro, *F > D-Fng-Myc *F > ========= *F > In fig.5 you mention D-Fng-Myc. We have a similar construct in our *F > records, but it is not attributed to any of the authors of your paper. *F > *F We made our own Myc tagged UAS-fng. A single Myc tag is inserted after the *F last codon of the Fng ORF. *F > fng-GAL4 *F > ======== *F > You also mention a fng-GAL4 line in fig.5. I cannot find any fng-GAL4 *F > lines in our records. Could you give me some more information about *F > this line? Is it a promoter fusion or an enhancer trap? If it is the *F > former can you give me some molecular details about the transposon *F > used, if the latter can you tell me which transposon was used to make *F > this insertion, whether the line has been published previously, and any *F > name that has been used to describe this line. *F > *F fng-Gal4 is an enhancer trap insertion. I received it as a gift from Juan *F Botas. The reason I did not respond sooner is I have been waiting for him *F to get back to me as regards to the transposon, but he hasn't done so. It is *F marked with w+, so I guess it is probably GawB. To my knowledge it has never *F been published previously. *F Let me know if you need me to try to get your more information than that. *F Sincerely, *F Ken Irvine *F Howard Hughes Medical Institute *F Waksman Institute *F Rutgers, The State University of New Jersey *F 190 Frelinghuysen Rd *F Piscataway NJ 08854-8020 *F (732) 445-2332 office phone *F (732) 445-2872 lab phone *F (732) 445-5735 Institute FAX *F irvine@waksman.rutgers.edu *F To: irvine@waksman.rutgers.edu *F Subject: FlyBase Query (cy755) *F From: Chihiro Yamada *F Date: Mon, 7 Aug 2000 14:33:13 \+0100 *F Dear Dr Irvine, *F I am currently curating your paper for FlyBase: *F Moloney et al., 2000, Nature 406(6794): 369--375 *F I have a couple of questions I was hoping you could answer for me. *F D-Fng-Myc *F ========= *F In fig.5 you mention D-Fng-Myc. We have a similar construct in our *F records, but it is not attributed to any of the authors of your paper. *F fngScer\UAS.T:Hsap\MYC *F Munro and Freeman, 2000, Curr. Biol. 10(14): 813--820 *F Ju et al., 2000, Nature 405(6783): 191--195 *F P{UAS-fng.MYC} *F The @fng@ cDNA is driven by @Scer\UAS@ sequences and tagged *F with @T:Hsap\MYC@. *F Is this the one you used, or did you make your own Myc tagged UAS-fng? *F If the latter is the case can you give me some molecular details for *F this construct, and any other information you think might be useful to *F FlyBase users. *F fng-GAL4 *F ======== *F You also mention a fng-GAL4 line in fig.5. I cannot find any fng-GAL4 *F lines in our records. Could you give me some more information about *F this line? Is it a promoter fusion or an enhancer trap? If it is the *F former can you give me some molecular details about the transposon *F used, if the latter can you tell me which transposon was used to make *F this insertion, whether the line has been published previously, and any *F name that has been used to describe this line. *F Any new information that you give me will be curated as a personal *F communication from you to FlyBase, as long as you're happy with this. *F Best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0131042 *a Degtyarenko *b K. *t 2000.7.17 *T personal communication to FlyBase *u FlyBase error report for CG2140 on Mon Jul 17 03:18:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Jul 17 11:12:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 17 Jul 2000 11:12:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 17 Jul 2000 03:18:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kirill@ebi.ac.uk *F Subject: FlyBase error report for CG2140 on Mon Jul 17 03:18:10 2000 *F Content-Length: 1354 *F Error report from Kirill Degtyarenko (kirill@ebi.ac.uk) *F Gene or accession: CG2140 *F Missed gene *F Comments: The product of gene CG2140 is microsomal cytochrome b5. I presume the *F gene symbol should be CYB5. *F Cytochrome b5 is not an enzyme itself but an electron transfer protein *F in several enzyme systems, including P450 monooxygenase system. *F Although there is a number of other genes with cytochrome b5 homology, *F CG2140 product (TrEMBL: Q9V4N3) is a protein of exactly the same length *F (76% identity) as spectroscopically and functionally characterised *F microsomal cyt b5 from house fly [SWISSPROT:P49096; reference: *F Guzov et al. (1996) JBC 271: 26637-26645] *F BLAST result: *F Q9V4N3: 1 MSSEETKTFTRAEVAKHNTNKDTWLLIHNNIYDVTAFLNEHPGGEEVLIEQAGKDATENF 60 *F MSSE+ K FTRAEVAK+NT W \+IHNN+YDVTAFLNEHPGGEEVLIEQAGKDATE+F *F P49096: 1 MSSEDVKYFTRAEVAKNNTKDKNWFIIHNNVYDVTAFLNEHPGGEEVLIEQAGKDATEHF 60 *F Q9V4N3: 61 EDVGHSNDARDMMKKYKIGELVESERTSVAQKSEPTWSTEQQTEESSVKSWLVPLVLCLV 120 *F EDVGHS+DAR+MMK+YK+GELV ER++V \+KSEPTW+TEQ+TEESS+KSWL+P VL LV *F P49096: 61 EDVGHSSDAREMMKQYKVGELVAEERSNVPEKSEPTWNTEQKTEESSMKSWLMPFVLGLV 120 *F Q9V4N3: 121 ATLFYKFFFGGAKQ 134 *F ATL YKFFFG Q *F P49096: 121 ATLIYKFFFGTKSQ 134 *F Also see http://ag.arizona.edu/p450/CYTB5.html *F Browser: Mozilla/4.73 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131043 *a Siekhaus *b D. *t 2000.7.20 *T personal communication to FlyBase *u *F From siekhaus@uclink4.berkeley.edu Thu Jul 20 22:08:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 20 Jul 2000 22:08:46 \+0100 *F Mime-Version: 1.0 *F X-Sender: siekhaus@uclink4.berkeley.edu *F Date: Thu, 20 Jul 2000 14:14:30 \-0700 *F To: flybase-help@morgan.harvard.edu *F From: Daria Siekhaus *F Subject: update to info on amontillado *F Dear Flybasers, *F . *F . *F I had an update to the info on the amontillado gene (located at *F 97C3). A new paper has emerged which shows the gene actually has *F proteolytic activity, cleaving after KR sites. *F . *F . *F Best wishes, *F Daria *F Here's the info: *F J Biol Chem 2000 Jun 9;275(23):17886-9 *F Interaction of drosophila melanogaster prohormone convertase 2 and *F 7B2. INSECT CELL-SPECIFIC PROCESSING AND SECRETION Hwang JR, *F Siekhaus DE, Fuller RS, Taghert PH, Lindberg I *F . *F . # *U FBrf0131046 *a Misra *b S. *t 2000.8.11 *T personal communication to FlyBase *u *F From sima@fruitfly.bdgp.berkeley.edu Fri Aug 11 21:43:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 11 Aug 2000 21:43:54 \+0100 *F Date: Fri, 11 Aug 2000 13:50:11 \-0700 (PDT) *F From: Sima Misra *F X-Sender: sima@fruitfly *F To: Aubrey de Grey *F cc: curators@morgan.harvard.edu *F Subject: Re: Current list of possibly CG-able known genes *F MIME-Version: 1.0 *F > > > >Pros26S-RS6A AQ026194 insertion at base 246 in the 253 bp *F > > > =l(3)04210 using Joe's BLASTN analysis of GadFly *F > > > =CG10370|FBan0010370|CT29122|FBan0010370 last_updated:000321(1773bp) *F > > > (reverse orientation at nt 441 of transcript) *F > > *F > > This has cytology probs: Pros26S-RS6A is in 89A but Rpt5 = CG10370 *F > > in 95B. I see that Rpt5 is a 26S proteasome regulatory subunit 6A *F > > but the cytology of Pros26S-RS6A is from l(3)04210. Help! *F > *F > I've asked Todd to check this cytology, but it looks like while there is a *F > slide for l(3)04210, there is no cytology in BFD, which makes me think *F > the cytology at 89A is suspect. *F This is from Todd: *F \--- *F I looked at the the slide for l(3)04210 again and saw two sites. One *F at 89A1-2 as originally reported and another at 95B1-2. *F \--- *F So it looks like it really is 95B. How satisfying! *F cheers, *F sima # *U FBrf0131047 *a Kraut *b R. *t 2000.8.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Aug 14 15:12:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 14 Aug 2000 15:12:14 \+0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Version 4.3.2 *F Date: Mon, 14 Aug 2000 09:17:27 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Df(2R)D4 and Df(2R)E2 *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Stock Center *F Subject: Df(2R)D4 and Df(2R)E2 *F The following information accompanied stocks donated by Rachel Kraut, Cal *F Tech (6/00). *F Df(2R)D4 has the breakpoints 57A1-3;57B13. It complements shg mutations *F and Df(2R)F36, but fails to complement insc mutations. *F Df(2R)E2 has the breakpoints 57B1;57B13-14. It fails to complement shg and *F insc mutations, but complements Df(2R)F36. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0131048 *a Palter *b K. *t 2000.8.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Aug 14 16:29:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 14 Aug 2000 16:29:15 \+0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Version 4.3.2 *F Date: Mon, 14 Aug 2000 10:34:30 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Palter insertions *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Stock Center *F Subject: Palter insertions *F The following information accompanied stocks donated by Karen Palter, *F Temple University (7/00). *F 1. The following insertions are all on the second chromosome and are all *F homozygous viable and fertile: *F P{w+mC=UAS-Hsc70-3.D231S}D *F P{w+mC=UAS-Hsc70-4.D206S}E *F P{w+mC=UAS-Hsc70-4.K71S}G *F P{w+mC=UAS-Hsc70-3.K97S}D *F P{w+mC=UAS-Hsc70-3.WT}B *F P{w+mC=UAS-Hsc70-4.WT}B *F Their construction and transformation was described in Elefant and Palter, *F Molec. Biol. Cell 1999 10(7):2101--2117 (FBrf0109657). *F 2. P{Mhc-lacZ.H} insertions *F P{Mhc-lacZ.H}85-21-2 is a homozygous viable and fertile, X chromosome *F insertion. *F P{Mhc-lacZ.H}76-25-Y is a homozygous viable and fertile, third chromosome *F insertion. *F The construction and transformation of P{Mhc-lacZ.H} was described in Hess *F et al. (FBrf0066429). The vector was 'pi18' and the authors selected for *F transformants with G418, indicating that the vector must carry a neo *F resistance gene. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0131049 *a Steward *b R. *t 2000.8.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Aug 14 18:18:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 14 Aug 2000 18:18:15 \+0100 *F X-Sender: kcook@sunflower.bio.indiana.edu *F X-Mailer: QUALCOMM Windows Eudora Version 4.3.2 *F Date: Mon, 14 Aug 2000 12:23:28 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: l(2)W mutations *F Mime-Version: 1.0 *F From: Kevin Cook, Bloomington Stock Center *F Subject: l(2)W mutations *F The following information accompanied stocks donated by Ruth *F Steward, Rutgers University (7/00). *F The following EMS alleles were isolated in Liu and Steward, Development *F 126(20): 4477--4488, 1999 (FBrf0111416). *F Locus Alleles *F l(2)W1 1A2, 15C6, 15C10, 22F3, F19.10, J19.16, A22.4, D22.11 *F l(2)W2 4A5, D9.15, C18.10 *F l(2)W3 B *F shark 1 (shark1 was originally l(2)W4G) *F l(2)W5 11F5, 16G4, N, P, D6.8, H9.9, B10.5, E14.1, F15.2, C16.16 *F l(2)W6 24J11, 25B14, 26C14 *F l(2)W7 J10.3 *F Lis1 21C14, 23F2, D, G10.14 *F l(2)W9 B15.6, D19.8, D20.8 *F l(2)W10 7D14, 9A10, 25A6, 28A11, C, J, D6.4 *F l(2)W11 3B11, 12C14, 14E5, S, B1.1, B10.15, C10.3, G22.4 *F Khc** 6D10, 12C1, 10B12, 7J9, 16E8, A4.12, G5.8, K5.14, B14.5, I14.6, A20.6 *F l(2)W13 27E5, K, A16.14, F18.5, C23.5 *F l(2)W14 H, I1.14 *F l(2)W15 I *F l(2)W16 O *F l(2)W17 J3.8 *F l(2)W18 C20.16 *F l(2)W19 A10.15 *F \*\*l(2)W12 is a synonym of Khc, so the gene entries should be merged. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0131064 *a Misra *b S. *t 2000.8.21 *T personal communication to FlyBase *u *F From sima@fruitfly.bdgp.berkeley.edu Mon Aug 21 22:05:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 21 Aug 2000 22:05:18 \+0100 *F Date: Mon, 21 Aug 2000 14:11:47 \-0700 (PDT) *F From: Sima Misra *F X-Sender: sima@fruitfly *F To: flybase-updates@morgan.harvard.edu *F Subject: Rename gene *F MIME-Version: 1.0 *F We have discovered that the gene BcDNA:GH07346 is in fact *F BcDNA:GH07089--the clone that was sequenced full-length and whose sequence *F was submitted to GenBank with accession AF145640 was unfortunately not *F clone GH07346, but clone GH07089. We will be resubmitting this *F information to GenBank shortly. *F Thanks for your help correcting this information in FlyBase. *F Sima Misra *F FlyBase BDGP # *U FBrf0131065 *a Holdener *b B. *t 2000.7.28 *T personal communication to FlyBase *u FlyBase error report for CG1364 on Fri Jul 28 13:35:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Jul 28 21:29:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 28 Jul 2000 21:29:36 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 28 Jul 2000 13:35:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: holdener@life.bio.sunysb.edu *F Subject: FlyBase error report for CG1364 on Fri Jul 28 13:35:16 2000 *F Content-Length: 809 *F Error report from Bernadette Holdener (holdener@life.bio.sunysb.edu) *F Gene or accession: CG1364 *F Gene annotation error *F Gene CG1364 should be split. *F Comments: Although it is possible that the N-term and C-term 1/2s of this *F predicted protein are part of the same gene, similarity to two distinct genes *F in vertebrates suggest that CG1264 may merged two Drosophila genes. The *F N-terminal predicted amino acids (PAWAKKDRD.....NAKKDELCSTKK) are 48% *F identical to the novel human protein KIAA0081 and several mouse ESTs which are *F candidates for the mouse mesoderm development (mesd) mutation. While the *F C-term 1/2 of the protein (VQAKIGPSILNAD....KELRDVVHSYLK) is similar to *F ribulose-phosphate 3-epimerase from many species. *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131066 *a Lengyel *b J.A. *t 2000.8.1 *T personal communication to FlyBase *u FlyBase error report for CG13505 on Tue Aug 1 13:50:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Aug 01 21:44:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 1 Aug 2000 21:44:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 1 Aug 2000 13:50:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jlengyel@ucla.edu *F Subject: FlyBase error report for CG13505 on Tue Aug 1 13:50:58 2000 *F Content-Length: 421 *F Error report from Judith A. Lengyel (jlengyel@ucla.edu) *F Gene or accession: CG13505 *F Missed gene *F Comments: CG13505 and CG6741 together correspond to the arc gene. work *F defining a >5 kb transcript that contains both CG6741 and CG1305 together is *F published. please see Developmental Biology, 221 pp. 419-434 (2000) *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131067 *a Micklem *b D. *t 2000.8.2 *T personal communication to FlyBase *u FlyBase error report for CG11790 on Tue Aug 1 20:31:33 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 02 04:25:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 2 Aug 2000 04:25:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 1 Aug 2000 20:31:33 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: dmicklem@cmgm.stanford.edu *F Subject: FlyBase error report for CG11790 on Tue Aug 1 20:31:33 2000 *F Content-Length: 730 *F Error report from David Micklem (dmicklem@cmgm.stanford.edu) *F Gene or accession: CG11790 *F Gene annotation error *F Gene CG11790 has incorrect exon/intron structure. *F Comments: EST GH08893 appears to be a full-length EST for this gene. Based on *F this EST, the gene prediction *F algorithm missed one exon of CG11790. *F Based on the numbering of AE003750, the gene limits should be 109190 \- 110903. *F The exon limits are: *F 109234-109366 (unchanged) *F 109473-109797 (unchanged) *F 109890-110051 (unchanged) *F 110113-110238 (4bp shorter than old version) *F 110720-110903 (new exon). *F This gene is similar to the Celegans gene D2092.4 throughout its length. *F Browser: Mozilla/4.08 (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131068 *a O'Hare *b K. *t 2000.8.2 *T personal communication to FlyBase *u FlyBase error report for CG7697 on Wed Aug 2 07:19:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 02 15:13:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 2 Aug 2000 15:13:27 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 2 Aug 2000 07:19:33 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: k.ohare@ic.ac.uk *F Subject: FlyBase error report for CG7697 on Wed Aug 2 07:19:32 2000 *F Content-Length: 789 *F Error report from Kevin O'Hare (k.ohare@ic.ac.uk) *F Gene or accession: CG7697 *F Gene annotation error *F Gene CG7697 has incorrect exon/intron structure. *F Comments: We have sequenced this gene and a full-length cDNA \- see Hatton et al *F Nucleic Acids Research 28:520-526 and AF170082 for details. We find 6 single *F base differences between our genomic sequence and that in AE003723 \- the 4 that *F fall in coding are all silent so these are probably strain polymorphisms. *F Our analysis shows only one intron and not the two shown in Gadlfy and AE003723. *F Our data suggest that the annotation should correspond to: *F mRNA join(<51264..51467,51531..>52715) *F gene <51264..>52715 *F CDS join(51331..51467,51531..52653) *F Browser: Mozilla/4.73 [en] (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131069 *a McKearin *b D. *t 2000.8.3 *T personal communication to FlyBase *u FlyBase error report for CG17611 on Thu Aug 3 11:57:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 03 19:51:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 3 Aug 2000 19:51:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 3 Aug 2000 11:57:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mckearin@utsw.swmed.edu *F Subject: FlyBase error report for CG17611 on Thu Aug 3 11:57:32 2000 *F Content-Length: 480 *F Error report from dennis mckearin (mckearin@utsw.swmed.edu) *F Gene or accession: CG17611 *F Missed gene *F Comments: This transcript does not correspond to the bgcn gene. The correct *F bgcn lies approximately 10 kb away. See Ohlstein et al. (2000), Genetics 155: *F 1809-1819 for correct info about bgcn. I will also submit an annotation *F correction for the ESTs that represent bgcn. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131070 *a McKearin *b D. *t 2000.8.3 *T personal communication to FlyBase *u FlyBase error report for CG10330 on Thu Aug 3 12:29:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 03 20:25:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 3 Aug 2000 20:25:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 3 Aug 2000 12:29:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mckearin@utsw.swmed.edu *F Subject: FlyBase error report for CG10330 on Thu Aug 3 12:29:57 2000 *F Content-Length: 789 *F Error report from dennis mckearin (mckearin@utsw.swmed.edu) *F Gene or accession: CG10330 *F cDNA or EST error *F Comments: CG10330 and CG10331 are actually pieces a single transcript that *F represents the benign gonial cell neoplasm (bgcn) gene. The bgcn mRNA is ~4020 *F nts. and encodes a protein of 1215 amino acids. Bgcn contains domains related *F to RNA helicases and Ankyrin repeats but is missing critical residues for ATP *F hydrolysis and RNA unwinding. An accession number will be available shortly. *F The bgcn gene includes an intronic gene of unknown function. The sequences for *F this intronic gene are included in GadFly as part of CG10330. See Ohlstein et *F al., Genetics 155: 1809-1819. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131071 *a White-Cooper *b H. *t 2000.8.7 *T personal communication to FlyBase *u FlyBase error report for CG2075 on Mon Aug 7 07:12:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Aug 07 15:07:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 7 Aug 2000 15:07:13 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 7 Aug 2000 07:12:47 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: helen.white-cooper@zoo.ox.ac.uk *F Subject: FlyBase error report for CG2075 on Mon Aug 7 07:12:46 2000 *F Content-Length: 633 *F Error report from helen white-cooper (helen.white-cooper@zoo.ox.ac.uk) *F Gene or accession: CG2075 *F cDNA or EST error *F Comments: The annotation is missing a 5' non-coding exon which we have found *F using 5' RACE analysis. The full length transcript is 1.85kb, confirmed by *F Northern blot analysis. The sequence of the full length transcript has been *F submitted to embl database accession number AJ277307. *F Also the estimated chromosomal location is inaccurate, I have in situ-ed the *F transcript and it maps to 63A3-4. # *U FBrf0131072 *a Huen *b D. *t 2000.8.14 *T personal communication to FlyBase *u FlyBase error report for CG9155 on Mon Aug 14 03:16:30 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Aug 14 11:10:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 14 Aug 2000 11:10:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 14 Aug 2000 03:16:30 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: smh1008@cus.cam.ac.uk *F Subject: FlyBase error report for CG9155 on Mon Aug 14 03:16:30 2000 *F Content-Length: 1100 *F Error report from David Huen (smh1008@cus.cam.ac.uk) *F Gene or accession: CG9155 *F Gene annotation error *F Gene CG9155 has incorrect exon/intron structure. *F Comments: The splice donor of exon 2 disagrees with the experimentally derived *F sequence from N.S. Morgan et al (1994) J. Mol. Biol. 239:347 [U07596]. The *F consequence is a frameshift that disrupts the ORF. *F When corrected, the cDNA as reported by Morgan et al becomes *F join(90951..91009,92375..92519,92581..92696,92753..93215,93278..95541,95604..95 *F 700,95781..95999) *F and the CDS becomes *F join(92394..92696,92753..93215,93278..95541,95604..95700,95781..95792) *F U07596 may possibly have an error in that when an attempt is made to align it *F against the genome sequence, the only alignment consistent with splice site *F constraints requires the insertion replacement of a GA between exon 1 and 2 *F with just A. Doing so extends the ORF further upstream. *F Application of the changes to your alternate predicted transcript also yields *F a different ORF. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131073 *a Whitfield *b E. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG8201 on Wed Aug 16 02:35:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 10:29:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 10:29:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 02:35:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG8201 on Wed Aug 16 02:35:26 2000 *F Content-Length: 1346 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG8201 *F Gene annotation error *F Gene CG8201 has incorrect exon/intron structure. *F Comments: par-1 is now known to have at least 3 isoforms, N1, N2 and N3, as *F described *F in Shulman et al, Cell 101:377-388(2000). They have distinct N termini and *F shared kinase domain. *F Celera annotation of isoform N1: AE003795; AAF57549 is incorrect but the *F correct DNA sequence is present for the correct translation (as shown by *F AF258462; AAF69801, from Shulman et al). So please correct the mRNA and *F CDS features (no coordinates, sorry!) *F Celera translation AE003795; AAF57551 corresponds to isoform N2 (Shulman et *F al: AJ293622; CAC00683) *F Celera translation AE003795; AAF57550 corresponds to isoform N3 (Shulman et *F al: AJ293623; CAC00684) *F Please note that for isoforms N2 and N3 Shulman et al only submitted the unique *F N terminal sequences, the shared kinase domain is not annotated. The kinase *F domain is also missing from the Celera translations. *F Also AAF57551 corresponds to CG11960, so please merge this gene with par-1. *F I think that is all for now. *F Apologies for the lack of detail regarding the splice events but I haven't had *F time to do any more than identify the Celera mistakes. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131074 *a Robertson *b H. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG1670 on Wed Aug 16 11:48:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 19:42:11 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 19:42:11 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 11:48:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG1670 on Wed Aug 16 11:48:11 2000 *F Content-Length: 860 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG1670 *F Gene annotation error *F Gene CG1670 has incorrect exon/intron structure. *F Comments: I think the translation should start somewhat later, which *F provides a good signal sequence for this odorant binding protein. My *F coding region is *F ATGACGAACCTTCTGCTAGCAGTGGCCTGCGCCGCCGTGCTGATGGGATCGGCGACGGCGGACGAGGAGGAGGGGTCCAT *F GACCGTGGACGAGGTGGTGGAGCTGATCGAGCCCTTTGGCGACGCCTGCACGCCAAAGCCGTCGAGGGAGAACATCGTCG *F AGATGGTGCTGAACAAGGAGGACGCCAAGCACGAGACCAAGTGCTTCCGCCACTGCATGCTGGAGCAGTTCGAGCTGATG *F CCCGAGGATCAGTTGCAGTATAACGAGGACAAGACGGTCGATATGATCAACATGATGTTCCCGGATCGCGAGGACGACGG *F CAGGCGCATCGTCAAGACCTGCAACGAGGAGCTAAAGGCCGAGCAGGACAAGTGCGAGGCAGCCCACGGGATCGCTATGT *F GCATGCTGCGCGAGATGCGCTCTTCGGGCTTCAAGATTCCCGAGATCAAGGAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0131075 *a Robertson *b H. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG12665 on Wed Aug 16 11:55:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 19:49:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 19:49:16 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 11:55:42 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG12665 on Wed Aug 16 11:55:42 2000 *F Content-Length: 942 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12665 *F Gene annotation error *F Gene CG12665 has incorrect exon/intron structure. *F Comments: This annotation needs a 5' exon to encode a decent signal *F sequence typical of these odorant binding proteins. This construction *F is also supported by the cDNA GH24525. My coding region is *F ATGATGCGGAGATCACAGATCGGTTTGCTCAGCAGGCTGCTGCTGTTGCTGCTGGTGGTGGAACTGACGCCCCCTGCTAT *F TCCGGTGCCCATGCGATCCTCACCCCAATCGCTGGCCCTACTGCGAGCACGGGATCAGTGCGGCAGGGAGCTGACTGCTG *F CCCAGCGTCTGCAGCTGGACAGGATGCAATTCGAGGATGCTGCCCATGTGCGTCACTATCTCCATTGCTTCTGGTCACGG *F CTGCAGCTCTGGCTGGATGAGACCGGATTCCAGGCACAGCGCATCGTTCAGAGTTTCGGCGGCGAGAGGCGTCTCAATGT *F GGAGCAGGCACTGCCAGCCATCAACGGGTGCAATGCGAAAACGAGCTCCAGAGGATCGGGCGCTCAGACAGTGGTCGACT *F GGTGTTTCCGTGCCTTTGTCTGCGTGCTGGCCACTCCAGTCGGTGAGTGGTACAAGCGCCACATGTCCGATGTCATCAAT *F GGGAATGCC *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131076 *a Robertson *b H. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG15129 on Wed Aug 16 14:39:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 22:33:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 22:33:05 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 14:39:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG15129 on Wed Aug 16 14:39:32 2000 *F Content-Length: 1402 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15129 *F Gene annotation error *F Gene CG15129 should be split. *F Comments: Here are my coding cDNAs for the two gene, each encoding a *F reasonable odorant binding protein family member. *F ATGTATTTTCGAGCCAGTTTGATGGCATTGCTTTGCCTCACTCTTAGTGAATTCGTTTCTAAAGCATGGACCCGATCGCT *F TTCCGTCTCGCTGAACATGTCGATGACACGAACCCTGGTTCCAGATCCGCCAAATGGAACAGAAAACAAACTCAGCCAGG *F AGATGCTGAGGGCTTGTATGCGTAGGACCGAGATCTCAATGTCGCAACTGAAACTCTTTCACATGAGCCTGATGAACAGC *F GACTACAATAATGACAACGATATAGCCCCTACGCCAGTTCAATCCATTGGCGATTGCTTTGTGAGCTGTCTGTATGAGAC *F CCTGGATTTGGATAGGTACAATGTCCTGCTGGAGGAGGCCTTTAAGAATCAGGTGCAAACGATCATACAGCATGAGAAGG *F CGGAGATCAAGGAGTGTAGTGATCTTCAGGGCAAAACACGATGCGAGGCAGCCTACAAGCTGCACCTGTGCTACAATCAC *F CTGAAAACTCTGGAGGCGGAGCAGCGTATCCGTGAGATACTTGAGCGGACCGAGGCGGAGAACGAGGGATTCGGTCCGGA *F GGGCAGCGACTTTATCGACGGCATCCAGCATTCCGGAGAAGCAATGACCACCGCTAAGTCGGAG *F and *F ATGAAACTTATCTACTTGTTGGTTGTATTCCTAATTTTCGCTCTAAGCGAACTAGTAGCGGGCCAGTCAGCTGCGGAATT *F GGCAGCCTACAAGCAAATTCAACAGGCCTGCATCAAGGAGCTGAATATTGCTGCCAGTGATGCTAATTTGCTGACCACCG *F ACAAGGAGGTGGCGAATCCCTCTGAGTCGGTGAAGTGCTATCACAGCTGCGTCTACAAGAAACTGGGTCTCCTGGGTGAC *F GATGGAAAGCCCAATACTGATAAGATCGTTAAGTTGGCCCAGATCCGTTTCAGCAGTCTGCCGGTGGATAAGCTAAAGAG *F TTTGCTTACCAGCTGCGGAACCACAAAGTCAGCCGCCACCTGTGACTTTGTCTACAACTATGAAAAGTGTGTTGTTAAGG *F GTATTAGTGCC *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131077 *a Robertson *b H. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG12944 on Wed Aug 16 14:47:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 22:41:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 22:41:07 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 14:47:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG12944 on Wed Aug 16 14:47:32 2000 *F Content-Length: 978 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12944 *F Gene annotation error *F Gene CG12944 has incorrect exon/intron structure. *F Comments: I think an earlier start codon should be used to give a *F decent signal sequence for this secreted odorant binding protein family *F member, plus the splice acceptor site should be earlier. My coding *F cDNA is *F ATGAATCGAGTTCTAGTGCTATTGCTGGTGCTTAAAATGTTCGCTTTGAGCGAGCACATCTTCTGTCAGTCCCGTTTCGC *F CAAGATAAACATCAATCTGGGACTAACCGTGGCTGATGAATCCCCCAAAACGATCACCGAGGAAATGATTCGCCTGTGCG *F GAGATCAAACGGATATATCCCTCAGGGAGTTGAACAAGTTGCAAAGGGAGGACTTCTCGGATCCCTCGGAATCGGTCCAG *F TGTTTCACCCATTGCCTCTACGAGCAAATGGGTCTCATGCACGATGGTGTTTTTGTGGAACGCGATCTATTCGGGCTTCT *F TTCCGATGTCAGTAATACCGATTACTGGCCAGAACGTCAATGCCACGCGATTCGTGGCAATAACAAATGTGAGACGGCCT *F ACAGGATTCATCAATGCCAACAGCAGTTGAAACAACAGCAACAGAACTTATTGGCCACCAAGGAGGTTGAGGTCACCACC *F ACACCAGCTGGATCCGATGAAACAAAACCT *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131078 *a Robertson *b H. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report on Wed Aug 16 14:58:06 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 22:51:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 22:51:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 14:58:06 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for new on Wed Aug 16 14:58:06 2000 *F Content-Length: 707 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new *F Missed gene *F Comments: I believe there is an unannotated gene encoding a member of *F the odorant binding protein family in gb|AE003584.1 *F My coding cDNA is *F ATGCGAGTGTTGCTGGCTTTTGTACTTCTGCTTGGCCTCTCAGTTTTGGCCACTAAGGAACCGGAAGAAGTTAAAATTGT *F AAGCGAGTGTGCCAAGGAGAACAATGTTCATAGGAAGAAGGCACTGGACCTTTTAATGAGCTATCGTTTGAAGAAGAAAA *F CCCACAACGTCATGTGCTTCATCAACTGCATCTTCGAGCGAACCAACATACTGCAGAAAGTTAAGGAAAAAGTTGTAAAG *F GAAAATCACAACTGCGACTCCATCAAGGACGCTGATAAGTGTGCAGAATCCTTCCAAAAATTTCAATGCTTGGTCAAGAT *F TGAGATGAAACGTGATAGTGCAGCAACGCGACATGTGCCACAAACAATGCCCAAACTT *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0131079 *a Robertson *b H. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report on Wed Aug 16 15:00:15 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 22:53:51 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 22:53:51 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 15:00:15 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for new on Wed Aug 16 15:00:15 2000 *F Content-Length: 682 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new *F Missed gene *F Comments: I believe there is an unannotated gene encoding a fine member *F of the odorant binding protein family in >gb|AE003812.1 *F My coding cDNA *F ATGAAAGTATTCATTGGCCTGGTTCTGTTGTTAGCTGTCACTACGCTGTCATCCGCTTTATTCGAATCTGAAGCGAACGA *F ATGTGCTAAAAAACTGGGAATTACCCCAGATTACTTCGAAAATTTTCCGCACAGCAGTCGGGTGAAGTGCTTTTACCACT *F GCCAAATGGAAAAACTTGAAATAATTGCCAATGGTGTGGTAACACCATTCGATTTGAAAGTATTGAACATATCACCGGAG *F AGCTATGATAAGTATGGTGTAAAGGTAAAACCATGCCTCAAACTATCGCATCGCGACAAATGTGAGCTCGGTTACTTGGT *F GTTCCAGTGCTTGAAACGAGAATTTAACTTG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0131080 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG15583 on Thu Aug 17 12:02:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 19:56:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 19:56:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:02:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG15583 on Thu Aug 17 12:02:55 2000 *F Content-Length: 1674 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15583 *F Gene annotation error *F Gene CG15583 has incorrect exon/intron structure. *F Comments: I think the first two exons need to be split off as a *F separate odorant binding protein family gene. My coding cDNA is *F ATGCAGTCCCAATCCCTCCTGCTGATCGTTGCAGCCGTTGCCACGTTTCTGGTGGCCCAGACTACGGCCAAGTTCCTGCT *F GAAGGACCACGCCGACGCAGAGAAGGCGTTCGAGGAGTGCCGTGAGGACTACTACGTGCCGGACGACATCTACGAGAAGT *F ACCTGAACTACGAGTTTCCCGCCCACCGGCGCACCAGCTGCTTCGTCAAGTGCTTCCTGGAGAAGCTTGAGCTGTTCTCG *F GAGAAGAAGGGATTTGACGAGCGCGCCATGATCGCCCAGTTCACCTCCAAGAGCAGCAAAGACCTGTCCACGGTCCAGCA *F CGGCCTGGAGAAGTGCATCGACCACAACGAGGCCGAGTCGGATGTCTGCACCTGGGCCAACCGAGTCTTCTCCTGCTGGC *F TGCCCATCAACCGCCACGTGGTGCGTAAGGTCTTCGCC *F The last exon is more complicated. It appears to have the coding *F capacity for two odorant binding proteins, however they may be fused or *F alternatively spliced. In either case there may be a 5' exon missing *F that encodes a signal sequence characteristic of these proteins. My *F coding cDNA for the first part is *F ATGAGTTCCCCTCGTGCGGTTCTAGTCAGCCTGTTCCTGATCTGCAGTCAGGCACTAGCTGACCTTTCTGGTGATGCCCA *F GACTCTGGAAAAGTGCCTGCGGCAACTTAGTTCGCCGGAGAGCATTGCTGGCGATCTTCGAAAGCTGGAACGGTATTCAT *F CTTGGACGCGGGAGGAGGTACCTTGCCTGATGCGCTGCTTGGCCAGAGAAAAGGGCTGGTTCGACGTGGAGGAAAACAAG *F TGGAGGCTCAAGCAACTGACTGAGGACCTGGGCGCCGATGTCTATAACTACTGCAGATTCGAGCTGCGCCGGATGGGGTC *F CGATGGCTGCAGCTTCGCCTATCGGGGACTCAGGTGCCTGAAGCAGGCCGAGATGCATGCGGGCACCAGCCTGAGCACAC *F TGCTACAGTGTTCCCGCCAGCTG *F I don't know how to deal with the second part, perhaps you can annotate it as *F an alternatively spliced product. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131081 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG15582 on Thu Aug 17 12:11:12 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:05:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:05:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:11:12 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG15582 on Thu Aug 17 12:11:12 2000 *F Content-Length: 1491 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15582 *F Gene annotation error *F Gene CG15582 has incorrect exon/intron structure. *F Comments: This gene, like CG15583, may be a fusion of two genes, or it *F might involve alternative splicing, or it may even be correct and *F encoded an obligate heterodimer. In any case, I would propose a much *F closer 5' exon that encodes a suitable signal sequence typical of these *F odorant binding proteins. Thus my first coding cDNA is *F ATGCAGATGAAAAGTGGAATATTAATAGCATTATGTCTATGCCTTTCGTTGAACGAAGGCCTGGCCCTTCTGGAGCACGA *F AGGCGAGACCATCAACAGATGCATCCAAAACTATGGCGGACTTACTGCGGAAAATGCCGAACGTCTAGAACGATTCAAGG *F AATGGTCGGATAGCTACGAGGAAATCCCCTGCTTCACGCGCTGCTATTTGTCCGAGATGTTCGACTTTTACAATAACTTA *F ACGGGCTTCAATAAGGACGGAATTGTGGGCGTCTTTGGAAGACCCGTCTACGAAGCCTGCCGAAAGAAATTGGAACTGCC *F ATTCGAATCAGGCGAGAGCAGCTGCAAACATGCCTACGAGGGCTTCCACTGCATCACCAACGTGAGTAGGCATTTGAATT *F TCACTGATCTTCATAGGTGA *F And a possible second coding cDNA, unless this is alternative splicing *F or a real fusion, is *F ATGCGTATTTACACTACATTACTTAATATATTACAGATGGAAAGCCACCCGTTCACGGTTATTGACAACATGCCAAACAT *F ATCCCCGTCGGCCAAGGATGCAATGAAGGACTGCCTTCAGGATGTCCACCAGGACGAGTGGAAGAGCTTCGATGCCTTCG *F CCTACTATCCTGTCAATGAACCGATTCCGTGCTTCACCCGGTGCTTCGTGGACAAGCTGCATATCTTCGAGGAGAAAACG *F CGTCTTTGGAAACTGGAGGCGATGAAGCAAAACCTGGGCATTCCGGCCAAAGGAGCTCGCATAAGGACCTGCCATCGGCA *F CCGCGGCAGGGACCGATGTGCCACATATTACAAACAGTTCACCTGCTACGCGATGGCTGTTTAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131082 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG13429 on Thu Aug 17 12:15:01 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:08:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:08:58 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:15:01 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13429 on Thu Aug 17 12:15:01 2000 *F Content-Length: 850 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13429 *F Gene annotation error *F Gene CG13429 has incorrect exon/intron structure. *F Comments: This odorant binding protein family gene needs a new 5' exon *F to encode a suitable signal sequence, and removal of the second exon to *F shorten the C-terminus. My coding cDNA is *F ATGTTGGACCAACTTACACTGTGTTTGTTGCTAAATTTTCTGTGCGCAAATGTTCTCGCTAACACTTCAGTATTTAATCC *F GTGTGTTTCGCAAAATGAGTTATCCGAATATGAAGCCCACCAAGTGATGGAGAATTGGCCAGTTCCGCCCATCGATCGGG *F CTTACAAATGCTTTCTAACATGCGTCCTCTTGGATTTGGGTCTGATTGATGAACGGGGTAATGTGCAGATCGATAAGTAC *F ATGAAATCCGGAGTGGTGGACTGGCAATGGGTGGCAATAGAGTTGGTAACATGTCGCATAGAATTCAGCGACGAAAGGGA *F TCTGTGCGAGCTATCATATGGAATCTTCAACTGCTTCAAGGATGTGAAGCTTGCGGCCGAGAAGTATGTTTCAATTAGTA *F ATGCAAAGTAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131083 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG13873 on Thu Aug 17 12:26:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:20:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:20:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:26:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13873 on Thu Aug 17 12:26:18 2000 *F Content-Length: 788 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13873 *F Gene annotation error *F Gene CG13873 has incorrect exon/intron structure. *F Comments: This member of the odorant binding protein family needs a *F nearby 5' exon to encode a suitable signal sequence. My coding cDNA is *F ATGAGGGCTACATTCGCATTGACTCTGTTGCTCGGCTGCCTTTCAGGAATTTTGGCGCAAGCCAACATAGACAGTTCGGT *F GTCCAAGGAACTGGTGACGGATTGCCTCAAGGAGAACGGTGTCACTCCCCAGGATCTGGCTGACTTGCAATCGGGCAAGG *F TGAAGGCCGAGGATGCCAAGGACAATGTGAAGTGCTCCTCACAGTGCATTCTGGTCAAGAGCGGTTTCATGGACTCCACT *F GGCAAACTGCTGACCGACAAGATTAAGTCTTACTATGCGAACTCGAACTTTAAGGATGTCATCGAAAAGGATTTGGACAG *F GTGCAGCGCGGTCAAGGGGGCCAATGCCTGTGACACTGCCTTCAAGATATTATCCTGCTTCCAGGCAGCCAATTAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131084 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG13874 on Thu Aug 17 12:27:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:21:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:21:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:27:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13874 on Thu Aug 17 12:27:55 2000 *F Content-Length: 789 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13874 *F Gene annotation error *F Gene CG13874 has incorrect exon/intron structure. *F Comments: This member of the odorant binding protein family needs a *F nearby 5' exon to encode a signal sequence. My coding cDNA is *F ATGAAGTTCACCCTATTCTGTATTGCTCTGGCAGCTTTTTTGTCCATGGGACAGTGTAATCCGGACTTTCGCCAAATAAT *F GCAACAGTGCATGGAGACCAACCAAGTGACCGAGGCTGATCTCAAGGAGTTCATGGCCAGCGGGATGCAGAGCAGTGCCA *F AGGAGAACCTCAAGTGCTACACCAAGTGCCTGATGGAGAAGCAGGGTCATCTCACCAATGGCCAGTTCAATGCTCAGGCT *F ATGCTCGACACTCTCAAAAATGTGCCTCAGATCAAGGACAAAATGGACGAGATTTCCTCGGGAGTGAATGCCTGCAAGGA *F CATCAAGGGAACCAACGATTGCGACACGGCCTTTAAGGTTACCATGTGCCTGAAGGAGCACAAGGCCATTCCAGGACATC *F ACTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131085 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG15883 on Thu Aug 17 12:22:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:16:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:16:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:22:54 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG15883 on Thu Aug 17 12:22:54 2000 *F Content-Length: 781 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG15883 *F Gene annotation error *F Gene CG15883 has incorrect exon/intron structure. *F Comments: A much closer 5' exon makes for a decent signal sequence on *F this secreted odorant binding protein family member. My coding cDNa is *F ATGAAGGTTGTGTGCAGCATAGCTGTACTATGGATTTGCTTGATAACTATGTGGCAATCAGCTGGCCGCGTTAACGCAGA *F GGGTTGCCTAAAGCACCACAATCTGACCAGTGCCCAAGTGCAGGCAGTGGCTCCATCCACTCCCGTTGCGGATGTTCCAG *F TGGCCGTTAAGTGCTATAGCCGGTGTCTGATCCAGGATTATTTCGGTGATGATGGGAAAATCGATCTGCAGAAGGTGGGA *F AAGCGAGGATCTCAAGAGGACCACGTGATTTTGTCCCAGTGTAAGCAGCAGTTCGATGGCGTCACCAATCTGGACACGTG *F CGACTATCCATACCTGATTCTCCAGTGTTATTTTAAGGGCAAGCAGAGTGGAACTATCGCCTCGTAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131086 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report on Thu Aug 17 12:30:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:24:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:24:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:30:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for new on Thu Aug 17 12:30:58 2000 *F Content-Length: 729 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new *F Missed gene *F Comments: There is an unannotated gene encoding a member of the odorant *F binding protein family in gb|AE003791.1. My coding cDNA is *F ATGCCTGAAAAAATGTCTCTAAGACTTGTACCGCATCTGGCTTGTATTATTTTTATTTTGGAAATTCAATTTAGAATTGC *F CGATTCTAACGATCCGTGCCCCCATAATCAAGGAATAGACGAAGATATAGCCGAATCAATTCTAGGTGACTGGCCTGCAA *F ATGTGGATTTGACTAGCGTGAAAAGGTCCCACAAGTGTTATGTGACCTGTATTTTGCAATATTACAATATTGTGACCGCT *F TCTGGTGAGATATTTCTGGACAAGTACTACGATACTGGAGTCATTGATGAATTGGCGGTGGCACCCAAAATCAATCGATG *F CCGATATGAGTTTAGAATGGAAACAGATTATTGTAGCCGAATTTTTGCTATATTCAATTGTTTAAGGCAAGAAATATTAA *F CAAAGTCA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0131089 *a Robertson *b H. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report on Thu Aug 17 12:35:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 20:28:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 20:28:57 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 12:35:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for new on Thu Aug 17 12:35:22 2000 *F Content-Length: 729 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: new *F Missed gene *F Comments: There is an unannotated member of the odorant binding protein *F family in gb|AE003792.1 *F My coding cDNA is *F ATGTTCATCTACAGACTTGTATTTATTGCGCCTCTGATTTTGTTATTGTTCAGCTTGGCCAAGGCTCGCCACCCCTTTGA *F TATATTTCATTGGAATTGGCAAGACTTTCAGGAGTGTCTACAAGTTAATAATATTACCATAGGAGAATATGAGAAATACG *F CGCGACACGAAACTTTGGATTACCTGCTCAACGAGAAAGTCGACTTGAGGTACAAGTGCAATATTAAATGTCAGCTGGAA *F AGGGATTCAACGAAATGGTTGAATGCTCAAGGCAGAATGGATTTGGATTTGATGAATACGACCGATAAGGCATCCAAATC *F CATTACCAAGTGCATGGAGAAGGCTCCCGAAGAACTTTGTGCGTACAGTTTTAGACTGGTGATGTGTGCATTTAAGGCTG *F GCCATCCCGTAATTGATTCGGAATAA *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0131091 *a Yang *b Z. *t 2000.8.19 *T personal communication to FlyBase *u FlyBase error report for CG6657 on Sat Aug 19 19:25:34 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Aug 20 03:19:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 20 Aug 2000 03:19:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 19 Aug 2000 19:25:34 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: zhaohai@mail.med.upenn.edu *F Subject: FlyBase error report for CG6657 on Sat Aug 19 19:25:34 2000 *F Content-Length: 362 *F Error report from Zhaohai Yang (zhaohai@mail.med.upenn.edu) *F Gene or accession: CG6657 *F Gene annotation error *F Gene CG6657 corresponds to AF211892 (FBgn) *F Comments: It seems to be the Drosophila gene 'veg' (accession: AF211892) *F submitted by Prokopenko et al. *F Browser: Mozilla/4.61 [en]C-CCK-MCD NECCK (Win98; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131094 *a Manning *b G. *t 2000.8.8 *T personal communication to FlyBase *u FlyBase error report for CG16973 on Mon Aug 7 19:27:41 2000. *F From gerard-manning@sugen.com Tue Aug 08 03:22:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 8 Aug 2000 03:22:16 \+0100 *F X-Sender: gerard-manning@sugen1 *F X-Mailer: QUALCOMM Windows Eudora Pro Version 4.0 *F Date: Mon, 07 Aug 2000 19:27:41 \-0700 *F To: flybase-help@morgan.harvard.edu *F From: Gerard Manning *F Subject: Merging a CG gene record with a real one *F Mime-Version: 1.0 *F Hi, *F The flybase genes misshapen (msn) and CG16973 are the same. In addition, the *F reference sequences for msn don't cover the protein-coding region of the gene, *F but this is available from genbank protein record gi|7512005 (the record cites *F the msn cloning paper, and the sequence in that matches the sequence in the *F genbank record). This sequence is a clear match to the protein sequence of *F CG16973. *F Best regards, *F \-Gerard Manning. # *U FBrf0131095 *a Wodarz *b A. *t 2000.7.26 *T personal communication to FlyBase *u FlyBase error report for CG10261 on Wed Jul 26 04:39:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Jul 26 12:33:38 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 26 Jul 2000 12:33:38 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 26 Jul 2000 04:39:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: wodarz@uni-duesseldorf.de *F Subject: FlyBase error report for CG10261 on Wed Jul 26 04:39:45 2000 *F Content-Length: 1040 *F Error report from Andreas Wodarz (wodarz@uni-duesseldorf.de) *F Gene or accession: CG10261 *F Gene annotation error *F Gene CG10261 has incorrect exon/intron structure. *F Comments: We have sequenced EST clone HL05754 and 3' RACE products of *F embryonic mRNA. *F Our sequence shows that two exon-intron boundaries are not correctly annotated: *F The 5' boundary of exon 2 is at position 190401 (and not 190477) *F The 3' boundary of exon 9 is at position 205765 (and not 205720) *F Numbering corresponds to genomic scaffold 142000013386047 *F The altered 3' boundary of exon 9 changes the C-terminus of the predicted gene *F product. *F The correct cDNA and protein sequence can be found under GenBank accession no. *F AF288482 *F The reference for this sequence information is as follows: Wodarz, A., *F Ramrath, A., Grimm, A. and Knust, E. (2000). Drosophila atypical protein *F kinase C associates with Bazooka and controls polarity of epithelia and *F neuroblasts. J. Cell Biol., in press. *F Browser: Mozilla/4.04 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131096 *a Dow *b J. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG15520 on Wed Aug 16 01:22:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 09:16:10 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 09:16:10 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 01:22:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: j.a.t.dow@bio.gla.ac.uk *F Subject: FlyBase error report for CG15520 on Wed Aug 16 01:22:32 2000 *F Content-Length: 508 *F Error report from Julian Dow (j.a.t.dow@bio.gla.ac.uk) *F Gene or accession: CG15520 *F Gene annotation error *F Gene CG15520 corresponds to AF203878 (FBgn) *F Comments: This gene encodes two putative neuropeptides of the CAP2b family, *F and was submitted to genbank on 10 Nov 99. *F Drosophila melanogaster neuropeptide precursor (capa) mRNA, complete cds *F gi|7453550|gb|AF203878.1|AF2038787453550 *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131097 *a Shearn *b A. *t 2000.8.17 *T personal communication to FlyBase *u FlyBase error report for CG9088 on Thu Aug 17 11:47:35 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 17 19:41:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 17 Aug 2000 19:41:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 17 Aug 2000 11:47:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: bio_cals@jhu.edu *F Subject: FlyBase error report for CG9088 on Thu Aug 17 11:47:35 2000 *F Content-Length: 261 *F Error report from Allen Shearn (bio_cals@jhu.edu) *F Gene or accession: CG9088 *F Missed gene *F Comments: We have named this gene little imaginal discs (lid). *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131098 *a Celniker *b S. *t 2000.8.16 *T personal communication to FlyBase *u FlyBase error report for CG9930 on Wed Aug 16 12:39:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 16 20:33:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 16 Aug 2000 20:33:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 16 Aug 2000 12:39:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: celniker@bdgp.lbl.gov *F Subject: FlyBase error report for CG9930 on Wed Aug 16 12:39:56 2000 *F Content-Length: 449 *F Error report from Susan Celniker (celniker@bdgp.lbl.gov) *F Gene or accession: CG9930 *F Gene annotation error *F Gene CG9930 corresponds to FBgn0008646 *F Comments: *F I think CG9930 is E5 (FBgn0008646). Two fasta files *F appear for the CG. The first contains the homeobox for E5, the second should *F probably be deleted. Sue *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131099 *a Nash *b H.A. *t 2000.8.14 *T personal communication to FlyBase *u FlyBase error report for CG12047 on Mon Aug 14 14:28:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Aug 14 22:21:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 14 Aug 2000 22:21:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 14 Aug 2000 14:28:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: nash@codon.nih.gov *F Subject: FlyBase error report for CG12047 on Mon Aug 14 14:28:14 2000 *F Content-Length: 756 *F Error report from Howard A. Nash (nash@codon.nih.gov) *F Gene or accession: CG12047 *F Gene annotation error *F Gene CG12047 corresponds to FBgn0002873 *F Comments: As described in PNAS 97:8122, the mushroom body defect (mud) gene *F (FBgn0002873) *F occupies most of the the genome currently assigned to CG12047. The intron-exon *F boundaries observed in mud cDNAs (detailed in GenBank AF174134 and AF209068) *F differ significantly from those annotated in the GenBank submission for CG12047. *F Most importantly, the GadFly CG12047 omits the exon that we believe starts the *F gene and includes instead exons (those of cDNA GM07807) that appear to belong *F to a separate gene. *F Browser: Mozilla/4.08 (Macintosh; U; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131107 *a Theopold *b U. *t 2000.7.21 *T personal communication to FlyBase *u *F From leyla@morgan.harvard.edu Fri Aug 25 16:31:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 25 Aug 2000 16:31:56 \+0100 *F Date: Fri, 25 Aug 2000 11:38:11 \-0400 (EDT) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: Personal communication from Uli Theopold *F To: cambridge-curators@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Cete_of_Badgers_541_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 37838 *F Dear Cambridge Curators, *F Attached (and also included in the body of this email) is a personal *F communication from Uli Theopold from Mon, 21 Aug 2000, correlating the *F lectins from FBrf0110927 with the Celera predicted genes. *F Theopold, U., Rissler, M., Fabbri, M., Schmidt, O., Natori, S. *F Insect glycobiology: a lectin multigene family in Drosophila melanogaster. *F Biochem. biophys. Res. Commun. 1999 261(3):923--927 *F _____________________________________________________________________ *F lectin-24Da AC004279a N/A *F lectin-24Db AC004371a CG2958 *F lectin-24Fa AC004373a N/A *F lectin-24Fb AC004373b N/A *F lectin-29Ca AC004423a CG17799 *F accessory gland protein AC004423b CG17797 *F lectin-21Ca AC004573a CG2826 *F lectin-21Cb AC004573b CG13686 *F lectin-22C AC004716a CG15378 *F lectin-28C AC004723a CG7106 *F lectin-24A AC005149a CG3410 *F lectin-30A AC005889a CG17011 *F lectin-galC1 AC007082a CG9976 *F lectin-37Da AC007082b CG9978 *F lectin-37Db AC007082c CG9978 *F lectin-33A AC007083a CG16834 *F lectin-44Ca AC007330a CG1656 *F lectin-46Cb AC007330b CG1652 *F CK02422a CG7763 *F Incilarin-like GH10831 CG8343 *F N/A: not annotated *F AC004279a: in genomic clone AE 003578: around positions 44000-43000 *F AC004373a: in genomic clone AE 003576: around positions 205000-204000 *F AC004373b: in genomic clone AE003576: around positions 175000-174000 *F _____________________________________________________________________ *F Mime-Version: 1.0 *F X-Sender: uli@nyctea.molbio.su.se *F Date: Fri, 1 Sep 2000 10:31:01 \+0200 *F To: Leyla Bayraktaroglu *F From: Uli Theopold *F Subject: Re: Lectins *F Dear Leyla, *F I realised there was a problem and looked into the region between our *F two predicted lectins (37Da and 37Db), which had been put together *F into one lectin with two domains during the annotation. I think a *F small exon was missed, which adds a potential signal peptide to the *F lectin 37Db (between pos. 240829-241039 in clone AE003662). This exon *F is predicted by Genie (96 version, which I used from BDGP from Nomi *F Harris) but seems to have been missed by the later version. I *F experienced similar problems with lectin 38Da before but the signal *F peptide there seems ok. *F The predicted sequence after in silico slicing for the lectin 37Db would be: *F MVKLLLLFLVCWSALPLESSPLGNRYNLEIGEKQYYISLAKTNWFEASNHCRQNGGFLLN *F LESREELELLSPHLHPAYSYWLSINDLGERGVYVSEATGLEAPFLNWSAGEPDNSSGYDR *F CVELWLSTTSFQMNDLPCYSSVAFICQLN *F The lectin 37Db would correspond to the aminoterminal half of CG9978, *F maybe a bit longer since the first stop codon is downstream of the *F splice site used in the annotation: *F MLKTLVQLFLVVAGFAPGFGYDKYTTHIQNGNPYNLTVDMTPFIKINESY *F YVFGQTKVNWYVAYENCRRLQSELVTFETAEEFDAIAAFLNARGDRSEHW *F TSGNDLGKTGTHYWFSNAQLVTIKRWAPKQPDNAGGREHCIHLGYIYGYS *F TEFQLNDRPCHNHASSLFKYICEAPKQETVSIVVWEK *F Hope I could help you *F Uli *F Ulrich Theopold, PhD *F Dept. of Molecular Biology ph.: \+46-8-16 41 81 *F Stockholm University fax: \+46-8-15 23 50 *F S-106 91 Stockholm, Sweden e-mail: uli@molbio.su.se # *U FBrf0131112 *a Misra *b S. *t 2000.8.25 *T personal communication to FlyBase *u *F From sima@fruitfly.bdgp.berkeley.edu Fri Aug 25 18:55:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 25 Aug 2000 18:55:49 \+0100 *F Date: Fri, 25 Aug 2000 11:02:17 \-0700 (PDT) *F From: Sima Misra *F X-Sender: sima@fruitfly *F To: Aubrey de Grey *F cc: curators@morgan.harvard.edu *F Subject: nc5 report, second installment *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='-559023410-851401618-967225668=:13947' *F Content-Length: 33026 *F Hi Aubrey, *F Here is the second installment of nc5 curations. I had substantial help *F from Guochun, who has placed most of our P insertions on the genomic *F sequence. Guochun provided me with data that I include in my report--here *F is the key from Guochun of what the various fields are: *F p_name: P insertion name. *F name: CG name *F gene: if the CG is a known gene, then here is the gene name *F ct_name: is the CT of the above CG, and being used to decide the *F relation to P insertion *F relation: is the relation of P insertion and the CG. There are 3 *F values for this field: inside, front, and behind. 'inside' *F is obvious, and if a P is inside a CG, then inside_intron *F or inside_exon field will indicate which intron/exon it *F actually inserts. I'll use following diagram to explain *F 'front' and 'behind' relation. *F ===> is CG with orientation. *F \------- is genomic sequence. *F Normally there are 4 CG around a given P insertion site. *F P insertion site *F | *F | *F front | behind *F ==========> V =======> *F 5' \----------------------------------------------> 3' *F 3' <---------------------------------------------- 5' *F <====== <======== *F behind front *F so each P insertion will have TWO CG with relation 'front' *F or 'behind'. *F You also can go to my P insertion Genescene launch page *F 'http://weasel.lbl.gov:94/cgi-bin/pins/test.pl' to see *F some examples. It'll help to explain the relation. *F r_orientation: is the relative orientation of P insertion alignment *F and CG. e.g. if CG is on plus strand, and P aligned on *F minus strand, then r_orientation is '-'. *F inside_intron: indicates which intron the P insertion inserts. 0 means not *F inside. *F inside_exon: indicates which exon the P insertion inserts. 0 means not *F inside. *F dist5: gives the distance from 5' end of CT to the P insertion *F site *F dist3: gives the distance from 3' end of CT to the P insertion *F site *F My personal comments after looking at the BFD report and GeneSeen are in *F brackets []. *F I also looked at each line in GeneSeen, and looked at the BFD insertion *F line report to check the genetics. I found many cases where insertions *F mapped to the same place, or very close together, but surprisingly (to me, *F anyways) many times these insertions complemented each other. I'm not *F sure what to do in cases where insertions map to the same transcription *F unit but complement; I'm sure you all are experts at this sort of *F situation, and will annotate appropriately. *F . *F . *F Let me know if anything is unclear. *F \-sima *F \------------------------------------------------------------------------------ *F -- *F >l(2)01528,l(3)rJ880,l(3)j12B4 sequenced insertions in repeat in genome *F >l(2)00231=? *F p_name l(2)00231 *F name CG4272 *F gene BcDNA:GH09817 *F ct_name CT9361 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 351 *F dist3 3998 *F [l(2)00231 complements l(2)k08232, even though they are only 115bp away from *F each other; l(2)k08232 is 236bp upstream of BcDNA:GH09817, which is nearest *F gene to l(2)00231] *F >l(2)00232 sequence=CG7664 *F p_name l(2)00232 *F name CG7664 *F gene crp *F ct_name CT23425 *F relation inside *F r_orientation \- *F inside_intron 2 *F inside_exon 0 *F dist5 18013 *F dist3 5643 *F . *F . *F >l(2)00248=l(2)01275=l(2)k17014=l(2)k07303=Ef1alpha48D *F p_name l(2)00248 *F name CG8280 *F gene Ef1alpha48D *F ct_name CT24517 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 42 *F dist3 2850 *F [most likely Ef1alpha48D, since insertion is only 42bp upstream of *F transcription start at 6892926; hotspot for insertion since l(2)01275 22bp *F upstream of l(2)00248, l(2)k17014 17bp upstream, and l(2)k07303 14bp upstream; *F l(2)01275 and l(2)k17014 are known to be alleles of Ef1alpha48D and both are *F further away (more 5'); none ever tested for complementation] *F >l(2)00629=? *F p_name l(2)00629 *F name CG13438 *F gene CG13438 *F ct_name CT32796 *F relation front *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 5530 *F dist3 4790 *F [l(2)00629 insertion not near any annotations, but hotspot for insertion, *F since l(2)00629, l(2)k07001, and l(2)k06409 within 7bp of each other; amazing *F l(2)00629 was complemented by l(2)k06409 & l(2)k07001] *F >l(2)01038=mm *F p_name l(2)01038 *F name CG10941 *F gene mm *F ct_name CT30649 *F relation inside *F r_orientation \+ *F inside_intron 2 *F inside_exon 0 *F dist5 38086 *F dist3 57869 *F [gene has 4 introns, this insertion in middle of largest (huge) intron] *F >l(2)01085=CG15426 *F p_name l(2)01085 *F name CG15426 *F gene *F ct_name CT35488 *F relation inside *F r_orientation \+ *F inside_intron 0 *F inside_exon 6 *F dist5 20251 *F dist3 3841 *F >l(2)01094=? *F p_name l(2)01094 *F name CG9403 *F gene CG9403 *F ct_name CT9101 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 3149 *F dist3 8614 *F p_name l(2)01094 *F name CG15234 *F gene CG15234 *F ct_name CT35171 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 3130 *F dist3 3358 *F [possible that l(2)01094=l(2)k03204 since they are inserted 288bp away from *F each other, but never tested for complementation (unless typo and l(2)k03404 *F that non-complements l(2)01094 should be l(2)k03204)] *F >l(2)01296=CG3186 *F p_name l(2)01296 *F name CG3186 *F gene CG3186 *F ct_name CT10685 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 493 *F dist3 1150 *F >l(2)01351=? *F p_name l(2)01351 *F name CG13109 *F gene *F ct_name CT32343 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 22258 *F dist3 23996 *F [no annotations nearby; hotspot for 10 insertions 10.7kb to right] *F >l(2)01424=CG3845 *F p_name l(2)01424 *F name CG3845 *F gene CG3845 *F ct_name CT12829 *F relation inside *F r_orientation \- *F inside_intron 0 *F inside_exon 1 *F dist5 4 *F dist3 7047 *F >l(2)01466=ATPCL=CG8322 *F p_name l(2)01466 *F name CG8322 *F gene ATPCL *F ct_name CT18257 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 937 *F dist3 6500 *F >l(2)01810=CG5304 *F p_name l(2)01810 *F name CG5304 *F gene CG5304 *F ct_name CT16877 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 334 *F dist3 7870 *F >l(2)01848=? *F p_name l(2)01848 *F name CG2672 *F gene Tkr *F ct_name CT9053 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 514 *F dist3 13063 *F [l(2)01848 is 514bp upstream of Tkr, but l(2)03263 is inserted 17bp closer and *F remarkably they complement] *F >l(2)01857=l(2)k00107=l(2)00681=CG2140 *F p_name l(2)01857 *F name CG2140 *F gene CG2140 *F ct_name CT6982 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 645 *F dist3 1587 *F [inserted in first intron just 10bp downstream of l(2)00681; l(2)k00107 is *F located just upstream of start of transcription of CG2140; none tested for *F complementation; l(2)00681 must be multiple insert line since one insert maps *F to 51B5 and doesn't complement ttv, but sequence maps to CG2140 at 43D3] *F >l(2)02045=CG11546 *F p_name l(2)02045 *F name CG11546 *F gene CG11546 *F ct_name CT36453 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 4301 *F dist3 5002 *F [in intron of transcript CT36453; upstream of 2 other transcripts, CT36451 and *F CT9385] *F >l(2)02074=CG1512 *F p_name l(2)02074 *F name CG1512 *F gene CG1512 *F ct_name CT3821 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 24 *F dist3 4050 *F [only 24bp upstream of transcription start] *F >l(2)02836, l(3)03928, l(3)04069 sequences all map to repeat in genome *F p_name l(2)02836 *F name CG6983 *F gene CG6983 *F ct_name CT21627 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 1055 *F dist3 6919 *F [must be multiple insert line since genetically insertion is at 53B1, but *F sequence maps to 66D1, and other 2 insertions are supposed to be on third, but *F instead sequences map to identical nucleotide] *F >l(2)03050=CG9350? *F p_name l(2)03050 *F name CG9350 *F gene CG9350 *F ct_name CT26565 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 203 *F dist3 961 *F [CG9350 is 203bp downstream but only evidence for gene was homology to EST, no *F gene prediction] *F >l(2)03105=l(2)k16702=? *F p_name l(2)03105 *F name CG12464 *F gene CG12464 *F ct_name CT32655 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 13822 *F dist3 14253 *F p_name l(2)03105 *F name CG18369 *F gene CG18369 *F ct_name CT41749 *F relation front *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 26306 *F dist3 24529 *F [nothing nearby; l(2)k16702 inserted at identical nucleotide; these were never *F tested for complementation] *F >l(2)03497=wun? *F p_name l(2)03497 *F name CG8804 *F gene wun *F ct_name CT4876 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 164 *F dist3 9085 *F [seems to be a hotspot, with 4 insertions within 219bp of each other, just *F upstream of wun; complementation never tested with l(2)k09507, which is *F inserted in first exon of wun] *F >l(2)03563=? *F p_name l(2)03563 *F name CG17390 *F gene CG17390 *F ct_name CT33481 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 572 *F dist3 7041 *F [CG17390 is only gene close by, but 572bp downstream] *F >l(2)03605=l(2)03832=? *F p_name l(2)03605 *F name CG17952 *F gene CG17952 *F ct_name CT39996 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 990 *F dist3 3746 *F [CG17952 990bp downstream; l(2)03832 inserted 1bp upstream of l(2)03605, but *F these were not tested for complementation] *F >l(2)03709=CG15081 *F p_name l(2)03709 *F name CG15081 *F gene CG15081 *F ct_name CT42565 *F relation inside *F r_orientation \+ *F inside_intron 0 *F inside_exon 1 *F dist5 20 *F dist3 2334 *F >l(2)03771=CG18323=CG14028 *F p_name l(2)03771 *F name CG18323=CG14028 *F gene *F ct_name CT41595=CT33587 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 141 *F dist3 619 *F [nothing else around but CG18323=CG14028 141bp downstream] *F >l(2)03832=l(2)03605=? *F p_name l(2)03832 *F name CG17952 *F gene CG17952 *F ct_name CT39996 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 991 *F dist3 3747 *F [see record for l(2)03605; start of transcription of CG17952 is 991bp *F downstream] *F >l(2)03996=CG8258 *F p_name l(2)03996 *F name CG8258 *F gene CG8258 *F ct_name CT8297 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 7 *F dist3 2159 *F [CG8258 is just 7bp before the start of transcription] *F >l(2)04008=? *F p_name l(2)04008 *F name CG6320 *F gene *F ct_name CT19694 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 1499 *F dist3 8603 *F p_name l(2)04008 *F name CG16874 *F gene Vm32E *F ct_name CT19798 *F relation front *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 2850 *F dist3 2499 *F [not close to anything, 1.5kb from start of transcription of CG6320] *F >l(2)04111=? *F p_name l(2)04111 *F name CG10871 *F gene *F ct_name CT30433 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 20188 *F dist3 28966 *F [nearest annotation, CG10871, is 20kb away] *F >l(2)04154=CG5935 *F p_name l(2)04154 *F name CG5935 *F gene EG:EG0003.6 *F ct_name CT18411 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 252 *F dist3 3886 *F [but complemented by l(2)k0997, which is inserted in second intron, 769bp *F downstream] *F >l(2)04329=Nacalpha *F p_name l(2)04329 *F name CG8759 *F gene Nacalpha *F ct_name CT25274 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 7 *F dist3 1158 *F >l(2)04493=smt3 *F p_name l(2)04493 *F name CG4494 *F gene smt3 *F ct_name CT14617 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 33 *F dist3 832 *F [also shown to non-complement l(2)04841, which is inserted in smt3] *F >l(2)04530=CG9342 *F p_name l(2)04530 *F name CG9342 *F gene CG9342 *F ct_name CT3751 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 36 *F dist3 5986 *F [maps only 36bp upstream of gene] *F >l(2)04535=l(2)k16713=tkv *F p_name l(2)04535 *F name CG14026 *F gene tkv *F ct_name CT33585 *F relation inside *F r_orientation \+ *F inside_intron 2 *F inside_exon 0 *F dist5 5237 *F dist3 17291 *F [l(2)04535 must be multiple insert line since in situ said it mapped to *F 42C1-42C2; sequence of l(2)04535 and l(2)k16713 maps to large second intron of *F tkv, 211bp apart; l(2)04535 shown to be an allele of tkv genetically, *F l(2)k16713 never tested for complementation but is also a known tkv allele] *F >l(2)04723 sequence=dock *F p_name l(2)04723 *F name CG3727 *F gene dock *F ct_name CT42218 *F relation inside *F r_orientation \+ *F inside_intron 1 *F inside_exon 0 *F dist5 798 *F dist3 6434 *F p_name l(2)04723 *F name CG3727 *F gene dock *F ct_name CT12313 *F relation inside *F r_orientation \+ *F inside_intron 1 *F inside_exon 0 *F dist5 827 *F dist3 6434 *F . *F . *F >l(2)04845=l(2)k00208=AGO1? *F p_name l(2)04845 *F name CG6671 *F gene AGO1 *F ct_name CT20708 *F relation inside *F r_orientation \- *F inside_intron 2 *F inside_exon 0 *F dist5 1210 *F dist3 8908 *F [l(2)04845 maps to second intron of CT20708, l(2)k08121 maps to third intron *F of CT20708, second intron of CT42234, and l(2)k00208 maps to first intron of *F CT20708; l(2)k08121 surprisingly complemented l(2)04845 genetically but *F l(2)k00208 not tested] *F >l(2)05070=CG8392 *F p_name l(2)05070 *F name CG8392 *F gene CG8392 *F ct_name CT18263 *F relation inside *F r_orientation \- *F inside_intron 0 *F inside_exon 1 *F dist5 9 *F dist3 957 *F [l(2)05070 in first exon] *F >l(2)05095=? *F p_name l(2)05095 *F name CG10248 *F gene Cyp6a8 *F ct_name CT28799 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 5329 *F dist3 7009 *F p_name l(2)05095 *F name CG17453 *F gene Cyp317a1 *F ct_name CT31993 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 5185 *F dist3 6742 *F [must be multiple insert line since in situ at 39E1-39E2 and sequence maps to *F 51D3; sequenced insertion not really near any gene] *F >l(2)05248=? *F p_name l(2)05248 *F name CG8297 *F gene CG8297 *F ct_name CT20148 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 12775 *F dist3 13826 *F p_name l(2)05248 *F name CG8291 *F gene CG8291 *F ct_name CT21678 *F relation front *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 9789 *F dist3 1070 *F [original in situ for both l(2)k02205 and l(2)05248 at 52D1-52D2 but inferred *F genomic sequence map is 52D9; insertion sequence maps 50bp from l(2)k02205, *F which surprisingly complements l(2)05248; not really near any gene] *F >l(2)05287=CG12050 *F p_name l(2)05287 *F name CG12050 *F gene CG12050 *F ct_name CT3661 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 10 *F dist3 7350 *F [insertion maps 10bp upstream of gene; in situ was at 39A1-39A2 but inferred *F genomic sequence map is 39B1] *F >l(2)05428=l(2)k06503=Cdk4/6 *F p_name l(2)05428 *F name CG5072 *F gene Cdk4/6 *F ct_name CT16072 *F relation inside *F r_orientation \- *F inside_intron 3 *F inside_exon 0 *F dist5 4371 *F dist3 1562 *F p_name l(2)05428 *F name CG5072 *F gene Cdk4/6 *F ct_name CT15896 *F relation inside *F r_orientation \- *F inside_intron 3 *F inside_exon 0 *F dist5 3765 *F dist3 1562 *F [l(2)05428 and l(2)k06503 map to third intron of gene within 71bp of each *F other; never tested for complementation] *F >l(2)k00107=l(2)01857=l(2)00681=CG2140 *F p_name l(2)k00107 *F name CG2140 *F gene CG2140 *F ct_name CT6982 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 36 *F dist3 2268 *F [insertion is located 36bp upstream of start of transcription of CG2140; other *F insertions map to intron, within 10bp of each other; none tested for *F complementation; l(2)00681 must be multiple insert line since one insert maps *F to 51B5 and doesn't complement ttv, but sequence maps to CG2140 at 43D3] *F >l(2)k00208=l(2)04845=AGO1? *F p_name l(2)k00208 *F name CG6671 *F gene AGO1 *F ct_name CT20708 *F relation inside *F r_orientation \+ *F inside_intron 1 *F inside_exon 0 *F dist5 560 *F dist3 9558 *F p_name l(2)k00208 *F name CG6671 *F gene AGO1 *F ct_name CT42234 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 1849 *F dist3 9558 *F [l(2)04845 maps to second intron of CT20708, l(2)k08121 maps to third intron *F of CT20708, second intron of CT42234, and l(2)k00208 maps to first intron of *F CT20708; l(2)k08121 surprisingly complemented l(2)04845 genetically but *F l(2)k00208 not tested] *F >l(2)k02205=? *F p_name l(2)k02205 *F name CG8297 *F gene CG8297 *F ct_name CT20148 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 12825 *F dist3 13876 *F p_name l(2)k02205 *F name CG8291 *F gene CG8291 *F ct_name CT21678 *F relation front *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 9839 *F dist3 1120 *F [original in situ for both l(2)k02205 and l(2)05248 at 52D1-52D2 but inferred *F genomic sequence map is 52D9; insertion sequence maps 50bp from l(2)k02205, *F which surprisingly complements l(2)05248; not really near any gene] *F >l(2)k03204=? *F p_name l(2)k03204 *F name CG9403 *F gene CG9403 *F ct_name CT9101 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 3437 *F dist3 8902 *F p_name l(2)k03204 *F name CG15234 *F gene CG15234 *F ct_name CT35171 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 2842 *F dist3 3070 *F [possible that l(2)01094=l(2)k03204 since they are inserted 288bp away from *F each other, but never tested for complementation] *F >l(2)k06409=? *F p_name l(2)k06409 *F name CG13438 *F gene CG13438 *F ct_name CT32796 *F relation front *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 5523 *F dist3 4783 *F [l(2)k06409 insertion not near any annotations, but hotspot for insertion, *F since l(2)00629, l(2)k07001, and this one within 7bp of each other; amazing *F since l(2)k06409 was complemented by l(2)00629 & l(2)k07001] *F >l(2)k07001=? *F p_name l(2)k07001 *F name CG13438 *F gene CG13438 *F ct_name CT32796 *F relation front *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 5525 *F dist3 4785 *F [l(2)k07001 insertion not near any annotations, but hotspot for insertion, *F since l(2)00629, l(2)k07001, and l(2)k06409 within 7bp of each other; amazing *F since all 3 complement] *F >l(2)k08121=AGO1? *F p_name l(2)k08121 *F name CG6671 *F gene AGO1 *F ct_name CT20708 *F relation inside *F r_orientation \- *F inside_intron 3 *F inside_exon 0 *F dist5 4098 *F dist3 6020 *F p_name l(2)k08121 *F name CG6671 *F gene AGO1 *F ct_name CT42234 *F relation inside *F r_orientation \- *F inside_intron 2 *F inside_exon 0 *F dist5 1689 *F dist3 6020 *F p_name l(2)k08121 *F name CG6671 *F gene AGO1 *F ct_name CT42236 *F relation inside *F r_orientation \- *F inside_intron 0 *F inside_exon 1 *F dist5 5 *F dist3 6020 *F [l(2)04845 maps to second intron of CT20708, l(2)k08121 maps to third intron *F of CT20708, second intron of CT42234, and l(2)k00208 maps to first intron of *F CT20708; l(2)k08121 surprisingly complemented l(2)04845 genetically but *F l(2)k00208 not tested; hard to determine why these are all in AGO1 but 2 *F complement] *F >l(2)k16702=l(2)03105=? *F p_name l(2)k16702 *F name CG12464 *F gene CG12464 *F ct_name CT32655 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 13822 *F dist3 14253 *F p_name l(2)k16702 *F name CG18369 *F gene CG18369 *F ct_name CT41749 *F relation front *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 26306 *F dist3 24529 *F [nothing nearby but l(2)03105 at same nucleotide; not tested for *F complementation] *F >l(3)03928, l(2)02836, l(3)04069 sequences all map to repeat in genome *F p_name l(3)03928 *F name CG6983 *F gene CG6983 *F ct_name CT21627 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 1055 *F dist3 6919 *F [must be multiple insert line, since insertion maps genetically to third, but *F sequence maps to 66D1, and other 2 insertions' sequence likewise map in wrong *F position at identical nucleotide] *F >l(3)04069, l(3)03928, l(2)02836 sequences all map to repeat in genome *F p_name l(3)04069 *F name CG6983 *F gene CG6983 *F ct_name CT21627 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 1055 *F dist3 6919 *F [must be multiple insert line, since insertion maps genetically to third, but *F sequence maps to 66D1, and other 2 insertions' sequence likewise map in wrong *F position at identical nucleotide] *F >l(3)j12B4,l(2)01528,l(3)rJ880 inserted in repeat in genome *F >l(3)rJ880,l(2)01528,l(3)j12B4 inserted in repeat in genome *F \------------------------------------------------------------------------------ *F -- # *U FBrf0131113 *a Kaufman *b T. *t 1987.4.1 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Aug 31 19:09:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 31 Aug 2000 19:09:29 \+0100 *F Date: Thu, 31 Aug 2000 13:08:41 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: pbIB6, pbIB9 and zenMAS1 *F To: flybase-updates@morgan.harvard.edu *F X-Mailer: dtmail 1.3.0 CDE Version 1.3 SunOS 5.7 sun4m sparc *F Content-Type: text *F X-Sun-Text-Type: ascii *F Content-Length: 404 *F Personal communication from: Thom Kaufman, Indiana University *F To: Bloomington Drosophila Stock Center *F Subject: pbIB6, pbIB9 and zenMAS1 *F Dated: 1 April 1987 *F Background: Allele information from the Bloomington stock list. *F Information communicated: *F pbIB6, also known as pb41 *F pbIB9, also known as pb42 *F zenMAS1, also known as zen8 *F These are EMS-induced alleles made by Mark Seeger. # *U FBrf0131114 *a Wieland *b C. *t 1994.10.19 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Aug 31 21:12:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 31 Aug 2000 21:12:26 \+0100 *F Date: Thu, 31 Aug 2000 15:11:38 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: Df(1)18.1.15 *F To: flybase-updates@morgan.harvard.edu *F X-Mailer: dtmail 1.3.0 CDE Version 1.3 SunOS 5.7 sun4m sparc *F Content-Type: text *F X-Sun-Text-Type: ascii *F Content-Length: 399 *F Personal communication from: Claudia Wieland, Institut fur *F Entwicklungsbiologie, Koln *F To: Bloomington Drosophila Stock Center *F Subject: Df(1)18.1.15 *F Dated: 19 October 1994 *F Information communicated: *F Df(1)18.1.15 was made by X-ray mutagenesis of the A27.1F1 chromosome, a *F P(ry+) insertion in 8C/D described in Genes and Devel. 3: 1288-1300. It *F is deficient for bands 8C10-8E1,2 and P(ry+). # *U FBrf0131115 *a Bazinet *b C. *t 1997.10.3 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Aug 31 22:06:40 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 31 Aug 2000 22:06:40 \+0100 *F Date: Thu, 31 Aug 2000 16:05:53 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: Chc1 and Chc4 *F To: flybase-updates@morgan.harvard.edu *F X-Mailer: dtmail 1.3.0 CDE Version 1.3 SunOS 5.7 sun4m sparc *F Content-Type: text *F X-Sun-Text-Type: ascii *F Content-Length: 366 *F Personal communication from: Chris Bazinet, St. John's University *F To: Bloomington Drosophila Stock Center *F Subject: Chc1 and Chc4 *F Dated: 3 October 1997 *F Information communicated: *F Chc1 is a strong loss-of-function allele. *F Chc4 is a partial loss-of-function allele with a semi-dominant effect on *F viability; more males are produced at 21oC or lower. # *U FBrf0131116 *a Degtyarenko *b K. *t 2000.8.2 *T personal communication to FlyBase *u FlyBase error report for CG17875 on Wed Aug 2 09:03:18 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Aug 02 16:57:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 2 Aug 2000 16:57:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 2 Aug 2000 09:03:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kirill@ebi.ac.uk *F Subject: FlyBase error report for CG17875 on Wed Aug 2 09:03:18 2000 *F Content-Length: 421 *F Error report from Kirill Degtyarenko (kirill@ebi.ac.uk) *F Gene or accession: CG17875 *F Missed gene *F Comments: The sequence should be annotated as pseudogene (cf. FlyBase *F FBgn0038034) *F David Nelson says [FBrf0126927]: *F '9f3p is missing an exon or two at the active site. *F This includes the heme binding cys that is essential for activity' *F Browser: Mozilla/4.73 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131117 *a Blair *b S. *t 2000.8.24 *T personal communication to FlyBase *u FlyBase error report for CG15671 on Thu Aug 24 11:51:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 24 19:45:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 24 Aug 2000 19:45:22 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 24 Aug 2000 11:51:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ssblair@facstaff.wisc.edu *F Subject: FlyBase error report for CG15671 on Thu Aug 24 11:51:57 2000 *F Content-Length: 643 *F Error report from Seth S. Blair (ssblair@facstaff.wisc.edu) *F Gene or accession: CG15671 *F Gene annotation error *F Gene CG15671 corresponds to AF288223 (cv-2) *F Comments: 1) in our recent paper we provide evidence that CG15671 is *F crossveinless 2. See Conley, C.A., Silburn, R., Singer, M.A., Ralston, A., *F Rohwer-Nutter, D., Olson, D.J., Gelbart, W. and Blair, S.S. (2000). *F Crossveinless 2 contains cysteine-rich domains and is required for high levels *F of BMP-like activity during the formation of the cross veins in Drosophila. *F Development 127, 3947-3959. *F Browser: Mozilla/4.61 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131118 *a Blair *b S. *t 2000.8.24 *T personal communication to FlyBase *u FlyBase error report for CG15671 on Thu Aug 24 11:53:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Aug 24 19:46:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 24 Aug 2000 19:46:57 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 24 Aug 2000 11:53:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ssblair@facstaff.wisc.edu *F Subject: FlyBase error report for CG15671 on Thu Aug 24 11:53:32 2000 *F Content-Length: 868 *F Error report from Seth S. Blair (ssblair@facstaff.wisc.edu) *F Gene or accession: CG15671 *F Gene annotation error *F Gene CG15671 has incorrect exon/intron structure. *F Comments: cDNA, exon-intron structure. Based on the sequence of a cDNA, from *F an imaginal disc library, containing the complete CDS, the exon-intron *F structure for CG15671 appears in error (see our sequence at AF288223). We *F find no indication of the putative first 12 bp exon (in AE003453, bases *F 272,576-272,587). Rather, we find two additional 5' exons (in AE003453, at *F bases 280,096-279,916 and 277,040-276,952), for a total of six exons. The *F other exons and splice sites appear correct. This is also the splicing *F predicted by Fgene. However, we have not yet ruled out the possibility of *F other splice varients. *F Browser: Mozilla/4.61 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131124 *a Whitfield *b E. *t 2000.9.1 *T personal communication to FlyBase *u FlyBase error report for CG10243 on Fri Sep 1 05:39:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Sep 01 13:32:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 1 Sep 2000 13:32:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 1 Sep 2000 05:39:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10243 on Fri Sep 1 05:39:11 2000 *F Content-Length: 624 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10243 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG10243. *F Comments: In AE003813 you have an mRNA feature for CG10243 (CT28789) which *F corresponds *F to Cyp6a19, but no CDS feature. *F The mRNA encodes the exact protein (start to stop codon) that has all motifs *F consistent with a cytochrome p450 protein. Please add the CDS feature so the *F protein translation will have a protein_ID and be loaded into TrEMBL. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131125 *a Whitfield *b E. *t 2000.9.1 *T personal communication to FlyBase *u FlyBase error report for CG12028 on Fri Sep 1 07:28:07 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Sep 01 15:21:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 1 Sep 2000 15:21:26 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 1 Sep 2000 07:28:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12028 on Fri Sep 1 07:28:07 2000 *F Content-Length: 835 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12028 *F Gene annotation error *F Gene CG12028 has incorrect exon/intron structure. *F Comments: dib cDNA has been sequenced by Chavez et al (AF237560; AAF60174) and *F comparison *F to the Celera sequence (AE003480; AAF47831) reveals prediction of 4 false 5' *F exons and the 5' splice site of intron 6 is further downstream than predicted. *F For translation start: *F FT CDS join(63863..64521,64579..64988,65055..65167,65223..65672, *F FT 66302..66830,66971..67515,67632..67829,67916..68035) *F > *F FT CDS join(66281..66830,66971..67515,67632..67829,67916..68035) *F and position 67515 is wrong (splice site) *F please update mRNA feature too. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131126 *a Whitfield *b E. *t 2000.9.1 *T personal communication to FlyBase *u FlyBase error report for Cyp12d1 on Fri Sep 1 14:37:28 2000. *F From eleanor@ebi.ac.uk Fri Sep 01 14:30:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 1 Sep 2000 14:30:13 \+0100 *F Date: Fri, 01 Sep 2000 14:37:28 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 [en] (Win95; I) *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: FlyBase error report for CG12392 *F Content-Transfer-Encoding: 7bit *F >From the genes file: *F \*a Cyp12d1 *F \*z FBgn0041339 *F \*x FBrf0126925 == Dunkov, 2000.6.29, personal communication to FlyBase *F \*E FBrf0126925 == Dunkov, 2000.6.29, personal communication to FlyBase *F . *F . *F \*c 47E *F \*Y cytochrome P450 12d1 *F \*f microsome ; GO:0005792 *F \# *F . *F . *F Cyp12d1 DNA is present in AE003827 on the complement between positions *F 57072-55338. CDS is somewhere between these positions, with introns. *F Translation is 521 aa protein: *F MNTLSSARSVAIYVGPVRSSRSASVLAHEQAKSSITEEHKTYDEIPRPNKFKFMRAFMPG *F GEFQNASITEYTSAMRKRYGDIYVMPGMFGRKDWVTTFNTKDIEMVFRNEGIWPRRDGLD *F SIVYFREHVRPDVYGEVQGLVASQNEAWGKLRSAINPIFMQPRGLRMYYEPLSNINNEFI *F ERIKEIRDPKTLEVPEDFTDEISRLVFESLGLVAFDRQMGLIRKNRDNSDALTLFQTSRD *F IFRLTFKLDIQPSMWKIISTPTYRKMKRTLNDSLNVAQKMLKENQDALEKRRQAGEKINS *F NSMLERLMEIDPKVAVIMSLDILFAGVDATATLLSAVLLCLSKHPDKQAKLREELLSIMP *F TKDSLLNEENMKDMPYLRAVIKETLRYYPNGLGTMRTCQNDVILSGYRVPKGTTVLLGSN *F VLMKEATYYPRPDEFLPERWLRDPETGKKMQVSPFTFLPFGFGPRMCIGKRVVDLEMETT *F VAKLIRNFHVEFNRDASRPFKTMFVMEPAITFPFKFTDIEQ *F Maybe you have this information stored somewhere already? *F Please reinstate the CDS feature when you do the reannotation. *F thanks *F Eleanor # *U FBrf0131127 *a Whitfield *b E. *t 2000.9.1 *T personal communication to FlyBase *u FlyBase error report for Cyp309a2 on Fri Sep 1 15:53:28 2000. *F From eleanor@ebi.ac.uk Fri Sep 01 15:46:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 1 Sep 2000 15:46:15 \+0100 *F Date: Fri, 01 Sep 2000 15:53:28 \+0100 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 [en] (Win95; I) *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: FlyBase error report for CG3247 *F Content-Transfer-Encoding: 7bit *F >From the genes file: *F \*a Cyp309a2 *F \*z FBgn0041337 *F \*x FBrf0126925 == Dunkov, 2000.6.29, personal communication to FlyBase *F \*E FBrf0126925 == Dunkov, 2000.6.29, personal communication to FlyBase *F . *F . *F \*c 22F *F \*Y cytochrome P450 309a2 *F \*f microsome ; GO:0005792 *F \# *F . *F . *F Cyp309a2 DNA is present in AE003582 on the complement around positions *F 27000-25000 (that is vague!). CDS is somewhere between these positions, *F with introns. *F Translation is 507 aa protein: *F MYILASLALILLHLLVLPIYLYLTWHHKYWRKRGLVTARPLTLLGTYPGLLTRKSNLVFD *F VQKIYDKYKGKHRAVGVFVTRQPQILVLDPELAHEVLVSNFRCYKDSLQSSYLRHAKWDK *F YARLNPFWASGQSWRRLRTDAQAGISGSRLRQAYNIWEQGGQMLTEYMTQQVAEKNNILE *F TRDLCFRYTAHVMADFIWGIDAGTLTRPMEQPNKVQEMASKWTSYAFYMLTLFMATIVAP *F CSRLLLRFRFYPKETDEFFSNLTKESIELRLKAGDSTRTDYLSHLLQLRDQKQATHDDLV *F GHALTVMLDGYDTSGTALLHALYYLAENPAVQQKLRVEILSCMASEKSLDFEKLSSLQYL *F EQCFNESLRLSSLIPQYTKVCTLPTVIRLSESKSLDVEVGMTIMIPNYQFHHDKQYFPEP *F EAFKPERFDNGAYQELMRKGIFLPFSDGPRICMGVPLAMLTLKSALVHILSNFQVVRGRD *F RLIPKGDSGFGVVLQGDVNLEYRRFFR *F Maybe you have this information stored somewhere already? *F Please reinstate the CDS feature when you do the reannotation. *F thanks *F Eleanor # *U FBrf0131128 *a Hewes *b R. *t 2000.9.5 *T personal communication to FlyBase *u FlyBase error report for CG4187 on Tue Sep 5 12:51:38 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Sep 05 20:44:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 5 Sep 2000 20:44:52 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 5 Sep 2000 12:51:38 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hewesr@pcg.wustl.edu *F Subject: FlyBase error report for CG4187 on Tue Sep 5 12:51:38 2000 *F Content-Length: 996 *F Error report from Randy Hewes (hewesr@thalamus.wustl.edu) *F Gene or accession: CG4187 *F Gene annotation error *F Gene CG4187 has incorrect exon/intron structure. *F Comments: The predicted cDNA sequence for CG4187 (CT13764) differs from *F the corresponding genomic scaffold sequence in AE003491 by a single *F base which affects a predicted splice site between transmembrane region *F 6 and transmembrane region 7. The corresponding amino acid sequence *F for this junction is KLAALSG*CEISPDLY, where the \* indicates the *F splice site (between AAs 239 and 240). The amino acid sequence to *F either side of this junction matches other GPCR proteins well, *F indicating that this is likely a valid site. The AE003491 sequence *F shows a frameshift between the donor and acceptor splice sites, which *F would not allow generation of the above amino acid sequence. Please *F let us know how you resolve this discrepancy. *F Browser: Mozilla/4.0 (compatible; MSIE 4.01; Windows NT) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131129 *a Whitfield *b E. *t 2000.9.6 *T personal communication to FlyBase *u FlyBase error report for CG12153 on Wed Sep 6 08:18:04 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Sep 06 16:11:27 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 6 Sep 2000 16:11:27 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 6 Sep 2000 08:18:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12153 on Wed Sep 6 08:18:04 2000 *F Content-Length: 600 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12153 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG12153. *F Comments: Celera annotation of Hira (AE003441; AAF46267) is missing a splice *F variant. *F There is a shorter isoform described in Llevadot, Biochem. Biophys. Res. *F Commun. 249:486-491(1998), sequences of both isoforms from this paper are: *F AF071881; AAC64041 *F AJ222709; CAA10954 *F Please add a CDS feature for the shorter isoform *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131130 *a Whitfield *b E. *t 2000.9.6 *T personal communication to FlyBase *u FlyBase error report for CG5320 on Wed Sep 6 08:43:04 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Sep 06 16:36:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 6 Sep 2000 16:36:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 6 Sep 2000 08:43:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5320 on Wed Sep 6 08:43:04 2000 *F Content-Length: 604 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5320 *F Gene annotation error *F Gene CG5320 has incorrect exon/intron structure. *F Comments: I wonder if the splice sites in AE003745; AAF56209 (Gdh) are correct *F as the *F conflicts to a cDNA sequence submitted by Papadopoulou and Louis (Z29062; *F CAA82304) occur at the splice sites for introns 1, 4 and 6. *F Also Celera annotation is missing the longer isoform as shown in Y11314; *F CAA72173, please add a CDS feature for this isoform. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131131 *a Dow *b J.A.T. *t 2000.9.7 *T personal communication to FlyBase *u FlyBase error report for CG15520 on Thu Sep 7 04:56:09 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Sep 07 12:49:41 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Sep 2000 12:49:41 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 7 Sep 2000 04:56:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: j.a.t.dow@bio.gla.ac.uk *F Subject: FlyBase error report for CG15520 on Thu Sep 7 04:56:09 2000 *F Content-Length: 518 *F Error report from Julian Dow (j.a.t.dow@bio.gla.ac.uk) *F Gene or accession: CG15520 *F Gene annotation error *F Gene CG15520 corresponds to AF203878 (FBgn) *F Comments: This gene encodes a neurohormone prepropeptide, that contains two *F new members of the CAP2b family of diuretic peptides that act through nitric *F oxide (hence the name capability, abbrev. capa). It also contains a PBAN-like *F neuropeptide sequence. *F (MS in preparation) *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131132 *a Whitfield *b E. *t 2000.9.7 *T personal communication to FlyBase *u FlyBase error report for CG12181 on Thu Sep 7 08:43:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Sep 07 16:37:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Sep 2000 16:37:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 7 Sep 2000 08:43:43 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12181 on Thu Sep 7 08:43:43 2000 *F Content-Length: 382 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12181 *F Gene annotation error *F Gene CG12181 has incorrect exon/intron structure. *F Comments: Celera annotation of Sgs4 is in conflict with that determined by *F EDGP: AL024484; *F CAA19673. *F One of you must be right! *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131133 *a Whitfield *b E. *t 2000.9.7 *T personal communication to FlyBase *u FlyBase error report for CG10790 on Thu Sep 7 08:50:32 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Sep 07 16:44:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Sep 2000 16:44:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 7 Sep 2000 08:50:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10790 on Thu Sep 7 08:50:32 2000 *F Content-Length: 582 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10790 *F Gene annotation error *F Gene CG10790 has incorrect exon/intron structure. *F Comments: C terminus of Pig1 as annotated by Celera AE003427; AAF45859 is in *F conflict *F with that annotated by EDGP AL024484; CAA19669 and unfortunately no published *F sequence agrees with either sequence! *F see: *F Hofmann and Korge, Chromosoma 96:1-7(1987) \- M24138; AAA28893 *F Furia et al, Chromosoma 101:49-54(1991) \- X15760; CAA33767 *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131134 *a Whitfield *b E. *t 2000.9.8 *T personal communication to FlyBase *u FlyBase error report for CG14753 on Fri Sep 8 04:50:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Sep 08 12:43:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 8 Sep 2000 12:43:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 8 Sep 2000 04:50:16 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG14753 on Fri Sep 8 04:50:16 2000 *F Content-Length: 403 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG14753 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG14753. *F Comments: Cyp6a15&PSgr; is known to be a pseudogene so please add this *F information *F to the CDS feature of AE003836; AAF59077. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131135 *a Whitfield *b E. *t 2000.9.11 *T personal communication to FlyBase *u FlyBase error report for CG2125 on Mon Sep 11 06:34:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Sep 11 14:27:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 11 Sep 2000 14:27:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 11 Sep 2000 06:34:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2125 on Mon Sep 11 06:34:20 2000 *F Content-Length: 537 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2125 *F Gene annotation error *F Gene CG2125 has incorrect exon/intron structure. *F Comments: Celera have chosen the wrong N terminal coding exon for ci. The *F correct exon *F has been defined by Ahmed et al, Gene 197:367-373(1997) (U66884; AAC47752). *F The correct sequence is present but a little more downstream than the one *F currently defined (no coordinates, sorry). *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131136 *a Lipsick *b J. *t 2000.9.11 *T personal communication to FlyBase *u FlyBase error report for CG9045 on Mon Sep 11 14:13:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Sep 11 16:46:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 11 Sep 2000 16:46:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 11 Sep 2000 08:53:02 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: lipsick@stanford.edu *F Subject: FlyBase error report for CG9045 on Mon Sep 11 08:53:02 2000 *F Content-Length: 1469 *F Error report from Joe Lipsick (lipsick@stanford.edu) *F Gene or accession: CG9045 *F Missed gene *F Comments: There appears to be another gene just 5' of Myb (between Myb *F and G-beta) that was not identified by the genome project scanning *F programs. The complement of nt 148686-149581 of AE003500 encodes two *F adjacent open reading frames (separated by a probable intron) that *F predict a protein with significant homology to a family of AlkB-related *F proteins. The best hit thus far is an EST from pig (BE232761.1) that *F gives hits in the 7e-30 range with tblastx. There is a similarly *F related EST from humans and also less closely related ESTs from other *F species. In particular, there is a reasonably strong similarity at the *F protein level to a gene from E. coli called AlkB that is involved in *F DNA damage-induced repair of alkylated bases. There is no Drosophila *F EST for the predicted fly gene in your database. However, in E. coli *F this gene is likely DNA damage-inducible and the same may be true in *F flies. This example suggests that a segment by segment tblastx of the *F fly genome against the entire EST database may yield additional new *F genes. I couldn't end this note without congratulating everyone at *F BDGP and CELERA for an OUTSTANDING EFFORT! My own novice fly lab has *F benefited tremendously from the Drosophila genome sequence and *F annotations. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 95; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From FlyBase-error@hedgehog.lbl.gov Mon Sep 11 22:07:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 11 Sep 2000 22:07:05 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 11 Sep 2000 14:13:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: lipsick@stanford.edu *F Subject: FlyBase error report for CG9045 on Mon Sep 11 14:13:56 2000 *F Content-Length: 711 *F Error report from Joe Lipsick (lipsick@stanford.edu) *F Gene or accession: CG9045 *F Missed gene *F Comments: Further remarks on the existence of an AlkB homologue between G-beta *F and Myb on 13F: *F Actually, there is an EST in flybase (LD02396) that appears to cover this *F AlkB-related gene (I hadn't searched your EST database for a while). The *F EST sequence suggests that the mRNA goes from at least nt 149975 \-148622. *F The open reading frame is likely to include at least from 149027 \- 148686 *F and from 149581 \- 149088 (based on aa homology), interrupted by a single *F intron not present in the cDNA clone. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows 95; DigExt) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131137 *a Whitfield *b E. *t 2000.9.14 *T personal communication to FlyBase *u FlyBase error report for CG2707 on Thu Sep 14 04:02:50 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Sep 14 11:55:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 14 Sep 2000 11:55:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 14 Sep 2000 04:02:50 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2707 on Thu Sep 14 04:02:50 2000 *F Content-Length: 882 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2707 *F Gene annotation error *F Gene CG2707 has incorrect exon/intron structure. *F Comments: fs(1)Ya: Celera have annotated as having 2 splice variants: *F AE003425; AAF45816 *F AE003425; AAF45817 *F This information is not present anywhere in the literature and examination of *F the CDS features reveals that one of the 'variants' is due to incorrect 5' *F intron splice site, compare: *F FT CDS join(79042..79119,79178..81226) *F FT /note='fs(1)Ya gene product [alt 1]' *F and *F FT CDS join(79079..79120,79178..81226) *F FT /note='fs(1)Ya gene product [alt 2] *F The correct splice site is shown in alt 2. *F So please delete AE003425; AAF45816 as this translation is 'rubbish'! *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131138 *a Whitfield *b E. *t 2000.9.20 *T personal communication to FlyBase *u FlyBase error report for CG1464 on Wed Sep 20 06:09:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Sep 20 14:09:25 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 20 Sep 2000 14:09:25 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 20 Sep 2000 06:09:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1464 on Wed Sep 20 06:09:22 2000 *F Content-Length: 534 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1464 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG1464. *F Comments: Celera have only annotated 1 isoform of ey. *F AE003843; AAF59318 translates the embryonic isoform (as described by Quiring et *F al, Science 265:785-789(1994)) but is lacking the larval isoform. *F Please add a CDS feature to translate the second isoform. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131139 *a Whitfield *b E. *t 2000.9.20 *T personal communication to FlyBase *u FlyBase error report for CG12345 on Wed Sep 20 06:55:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Sep 20 14:56:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 20 Sep 2000 14:56:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 20 Sep 2000 06:55:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12345 on Wed Sep 20 06:55:56 2000 *F Content-Length: 622 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12345 *F Gene annotation error *F Gene CG12345 has incorrect exon/intron structure. *F Comments: Cha is annotated in Celera: *F AE003723; AAF55587 *F AE003723; AAF55588 *F but translation is missing 5 exons. *F Please compare to sequence shown in Swiss-prot entry P07668, this shows *F revisions to the original sequence (M63724; AAA28406, Itoh et al, Proc. *F Natl. Acad. Sci. U.S.A. 83:4081-4085(1986)) published in Sugihara et al, *F J. Biol. Chem. 265:21714-21719(1990) *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131140 *a Liu *b H. *t 2000.9.22 *T personal communication to FlyBase *u FlyBase error report for CG14827 on Thu Sep 21 22:07:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Sep 22 06:07:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 22 Sep 2000 06:07:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 21 Sep 2000 22:07:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: haoliu@rci.rutgers.edu *F Subject: FlyBase error report for CG14827 on Thu Sep 21 22:07:26 2000 *F Content-Length: 469 *F Error report from Hao Liu (haoliu@rci.rutgers.edu) *F Gene or accession: CG14827 *F cDNA or EST error *F Comments: It seems to me there are two insertions in the sequence. I have *F sequenced several genomic *F sequence sequence of mei-P22, all of them don't have the two stretches of *F insertions you have *F in the gene. *F It appears that mei-P22 contains no intron, it encodes a protein of 318AA *F Browser: Mozilla/4.75 [en] (Win98; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131141 *a Brody *b T. *t 2000.9.6 *T personal communication to FlyBase *u FlyBase error report for CG12397 on Wed Sep 6 10:08:07 2000. *F From brodyt@codon.nih.gov Wed Sep 06 15:00:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 6 Sep 2000 15:00:02 \+0100 *F Mime-Version: 1.0 *F X-Sender: brodyt@codon.nih.gov (Unverified) *F Date: Wed, 6 Sep 2000 10:08:07 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: tom *F Subject: debcl and CG12397 *F Dear FlyBasers: *F I would like to report an error in the definition of CG12397. The *F sequence in the database is a composite of two genes 1) debcl and 2) *F a zinc finger transcription factor just adjacent to the terminus of *F debcl. I have isolated an EST that starts 250 bases downstream of *F the defined debcl gene. This corresponds to the start of the zinc *F finger protein that is not part of debcl. The zinc finger coding *F gene is likely to terminate at the end of the defined CG12397, but *F CG12397 as currently defined also includes the upstream debcl *F sequence, identified in several labs. The error is reflected in *F sequences in GadFly and NCBI. The reason for this error is that the *F very end of the debcl sequence appears to have a splice site 'GT' *F start site that predicts that the 3' end of the debcl gene is spliced *F out. I believe there is enough evidence in the literature for the *F debcl terminus to conclude that this GT does not start an intron. If *F in fact it does, then debcl is an alternatively spliced gene that *F includes a C-terminal zinc finger. I don't think this is true, *F particularly based on my EST sequence, and therefore suggest that *F CG12397 be redefined. *F Sincerely, *F Tom Brody # *U FBrf0131142 *a Gelbart *b W. *t 2000.9.30 *T personal communication to FlyBase *u *F From gelbart@morgan.harvard.edu Sat Sep 30 19:54:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 30 Sep 2000 19:54:31 \+0100 *F Date: Sat, 30 Sep 2000 14:55:13 \-0400 (EDT) *F From: William Gelbart *F To: flybase@morgan.harvard.edu *F Subject: gene merge: prd3 & vvl *F Content-Length: 1859 *F Folks, *F Here is a gene merge based on sequence identity and on consistent polytene *F location. Both *F sequences are presence once in the assembled genome. *F Based on usage, I strongly urge that vvl be the valid symbol. *F Cheers, *F Bill *F =============================================================================== *F ============= *F vvl (FBgn0003995) and prd3 (FBgn0003146) are the same gene. *F Sequence 1 X58435 *F gi 7717 *F D.melanogaster CF1-a mRNA for a POU-domain protein *F Length 2215 *F Sequence 2 M14551 *F gi 158173 *F D.melanogaster PRD gene 3 encoding a segmentation protein, partial. *F Length 114 *F NOTE:The statistics (bitscore and expect value) is calculated based on the *F size of nr database *F Score = 173 bits (90), Expect = 8e-41 *F Identities = 90/90 (100%), Positives = 90/90 (100%) *F Query: 2125 *F catccgcaccacccgcatccgcatccgcaccagcatccgcacctgaatcccgcccaccat 2184 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 *F catccgcaccacccgcatccgcatccgcaccagcatccgcacctgaatcccgcccaccat 60 *F segmentation protein PRD 3 1 H P H H P H P H P H Q H P H L *F N P A H H *F Query: 2185 ccgcacctgttccacacgagcgatcagttg 2214 *F |||||||||||||||||||||||||||||| *F Sbjct: 61 ccgcacctgttccacacgagcgatcagttg 90 *F segmentation protein PRD 3 21 P H L F H T S D Q L *F Also consistent with reported cytologies: *F prd3 = 65D *F vvl = 65C5--65D1 # *U FBrf0131143 *a Whitfield *b E. *t 2000.10.5 *T personal communication to FlyBase *u FlyBase error report for CG11924 on Thu Oct 5 03:32:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 05 11:32:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 5 Oct 2000 11:32:47 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 5 Oct 2000 03:32:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11924 on Thu Oct 5 03:32:45 2000 *F Content-Length: 588 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11924 *F Gene annotation error *F Gene CG11924 has incorrect exon/intron structure. *F Comments: C terminal sequence of Celera translation for Cf2 (AE003575; AAF50966) *F is incorrect. *F If the splice sites are corrected translation of C-terminus is 100% *F identical to that from Hsu et al, Science 257:1946-1950(1992) *F (M97196; AAA28395 and M97196; AAA28396) and Shea et al, Genes Dev. *F 4:1128-1140(1990) (X53380; CAA37460). *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131144 *a Whitfield *b E. *t 2000.10.5 *T personal communication to FlyBase *u FlyBase error report for CG2621 on Thu Oct 5 03:46:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 05 11:46:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 5 Oct 2000 11:46:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 5 Oct 2000 03:46:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2621 on Thu Oct 5 03:46:55 2000 *F Content-Length: 709 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2621 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG2621. *F Comments: Celera has 1 translation for sgg (AE003425; AAF45801) that does not *F match a *F known isform. It nearly matches sgg46 but has wrong splice sites defined. *F Two are known and described by Ruel et al, EMBO J. 12:1657-1669(1993) *F X70863; CAA50213 (sgg39 isoform) *F X70864; CAA50214 (sgg46 isoform) *F and the other two by Siegfried et al, Nature 345:825-829(1990) *F X54005; CAA37951 (zygotic isoform) *F X54006; CAA37952 (maternal isoform) *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131145 *a Whitfield *b E. *t 2000.10.6 *T personal communication to FlyBase *u FlyBase error report for CG4205 on Fri Oct 6 02:15:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Oct 06 10:17:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 6 Oct 2000 10:17:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 6 Oct 2000 02:15:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4205 on Fri Oct 6 02:15:14 2000 *F Content-Length: 614 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4205 *F Gene annotation error *F Gene CG4205 has incorrect exon/intron structure. *F Comments: Celera translation of Fdxh (AE003552; AAF50293) reads through a *F C-terminal *F splice site as defined by Pauli and Tonka, J. Mol. Biol. 198:235-240(1987) *F (X06542; CAB55551). *F The correct C-terminal sequence is present in the DNA but shows 2 amino acids *F in conflict with translation from X06542. *F Please update the CDS and mRNA feature to show correct exons. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131146 *a Whitfield *b E. *t 2000.10.6 *T personal communication to FlyBase *u FlyBase error report for CG10223 on Fri Oct 6 08:25:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Oct 06 16:25:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 6 Oct 2000 16:25:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 6 Oct 2000 08:25:19 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10223 on Fri Oct 6 08:25:19 2000 *F Content-Length: 994 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10223 *F Gene annotation error *F Gene CG10223 has incorrect exon/intron structure. *F Comments: Celera translation of Top2 (AE003663; AAF53802) introduces an intron *F where one *F has not previously been identified. *F Wyckoff et al, J. Mol. Biol. 205:1-13(1989) (X61209; CAA43523) does not have *F this intron and reads in frame through the proposed splice sites to encode an *F extra 24 amino acids. *F Please amend CDS feature: *F CDS join(complement(10306..10863),complement(10075..10248), *F complement(9825..10002),complement(6721..9758), *F complement(6316..6639)) *F > *F CDS join(complement(10306..10863),complement(9825..10248), *F complement(6721..9758), complement(6316..6639)) *F intron between 10002 and 10075 removed, please make the appropriate change to *F mRNA feature too. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131147 *a Butler *b H. *t 2000.10.9 *T personal communication to FlyBase *u FlyBase error report for CG12345 on Mon Oct 9 10:36:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Oct 09 18:36:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 9 Oct 2000 18:36:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 9 Oct 2000 10:36:11 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hb246@gen.cam.ac.uk *F Subject: FlyBase error report for CG12345 on Mon Oct 9 10:36:11 2000 *F Content-Length: 403 *F Error report from Heather Butler (hb246@gen.cam.ac.uk) *F Gene or accession: CG12345 *F Gene annotation error *F Gene CT41182 corresponds to FBgn0015323 *F Comments: CT23399 and CT41182 do not belong to the same gene. *F CT23399 is correctly attached to CG12345 (FBgn0000303) but *F CT41182 should be FBgn0015323. *F Browser: Mozilla/4.7 [en] (X11; I; SunOS 5.7 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131148 *a Millburn *b G. *t 2000.10.11 *T personal communication to FlyBase *u FlyBase error report for PNBP on Wed Oct 11 02:52:51 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Oct 11 10:52:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 11 Oct 2000 10:52:47 \+0100 *F Date: Wed, 11 Oct 2000 02:52:52 \-0700 (PDT) *F From: FlyBase-error@hedgehog.lbl.gov *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for PNBP on Wed Oct 11 02:52:51 2000 *F Content-Length: 1297 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: PNBP *F Gene annotation error *F Gene PNBP (FBgn0026784) corresponds to bgcn (FBgn0004581) *F Comments: PNBP (FBgn0026784) corresponds to bgcn (FBgn0004581). *F PNBP is described in: *F FBrf0108232 == Lukacsovich et al., 1999, Genomics 57(1): 43--56 *F where it is stated to correspond to two stretches of accession number *F AB010261, nucleotides 28,356-28,844 and 31,159-13,671. *F ClustalW alignment of these 2 stretches of nucleotides to the bgcn *F sequence (AF255662) shows good alignments of the two chunks to bgcn (I *F will send the ClustalW alignments in a separate e-mail to *F flybase-help). *F In addition, in the paper: *F Ohlstein et al., 2000, Genetics 155(4): 1809--1819 *F In Figure 1, the gene 'wibg' is shown in an intron of bgcn, transcribed *F in the same direction, and it is stated in this paper that 'wibg' *F corresponds to the 'UD3' transcript from FBrf0108232. *F Looking at Figure 1B. from FBrf0108232, 'UD3' (FlyBase ID: FBgn0027071) *F is located in an intron of PNBP, transcribed in the same direction. *F Together, these pieces of evidence provide good evidence that PNBP *F (FBgn0026784) corresponds to bgcn (FBgn0004581). *F Gillian *F Browser: Mozilla/4.7 [en] (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Wed Oct 11 10:55:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 11 Oct 2000 10:55:29 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: ClustalW of PNBP and bgcn *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 11 Oct 2000 10:55:17 \+0100 *F Content-Length: 45938 *F Hi, *F ClustalW alignments of PNBP sequences to bgcn (AF255662) as promised in *F FlyBase-error report: *F 1. PNBP sequences (taken from AB010261): *F >PNBP,28356-28844 *F tcgctgagcgacgagatcttgcagtcattgtacgccagtcgggtgatcgtctataacgctgcgctttgctgggacaagt *F ccgtcttcctgcccctggtcattctggacgattgccggaacaagaagtccaacgtcaagattatgtgcatcgagcggca *F ggccattctggccacctacaacagccagcgaacggccaacttcttcggcgagcagctgggcgagacggtgggcatccag *F ttgccgtacttcagcgccgttagctccagcacttttattatttactcgacggcgcagtactttttgcgctcgctgacca *F gccagcagtttcgcaacatcagtcacctggtggtgaacgatgtccacctgcatgatccctacaccgacatcctgttgag *F cgagatacgcatggcgctaagtagccatcagaacctgagggtggtgctgctgtcgcaaatgggaaaccccaagaagttc *F accgacttctttggc *F ===== *F >PNBP,31159-31671 *F tgcttccgtctgataagcaaggaggcctacgaggagctaagcgagaccagtcagccaagcctgcagactatgcaactgg *F acaagatctgcctggcggtgaaactactctcacccaatacgattataagcgaatatctgggtattaccatctcaccgcc *F accgctgatcaacgtgcaccatgccgtgcagttcctcaagaaaatagatgtgctcgatgacgccgaggatgtgacctgg *F ctgggctgccgcctgatggacatcccggtctcctgtcaactcggccgcatgctgatcttcggcatcctgctgcgctgcc *F tggatccaatactcacgattgtcagctctctgtcgacggcggacccgttgggcattccgtttaccgaggacatcgacaa *F cttgtgggacaggttcaccatctacatccagaacagcatcaagaaagagcggacctacctgtccgacaatcagttttcc *F gatcacttcatcttcgtgcgcttgtacaaggagtggcaa *F ==== *F ClustalW alignments: *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|9651990|gb|AF255662.1|AF255 4021 bp *F Sequence 2: PNBP_28356-28844 489 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 100 *F Guide tree file created: *F [/net/nfs0/vol1/production/w3nobody/tmp/96764.27755.dnd] *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:9291 *F Alignment Score 3662 *F CLUSTAL-Alignment file created *F [/net/nfs0/vol1/production/w3nobody/tmp/96764.27755.aln] *F Your guide tree: *F 96764.27755.dnd *F (gi|9651990|gb|AF255662.1|AF255:0.00000,PNBP_28356-28844:0.00000); *F Your Multiple Sequence Alignment: *F 96764.27755.aln *F CLUSTAL W (1.81) multiple sequence alignment *F gi|9651990|gb|AF255662.1|AF255 *F CACGACGGTGGCAATAACGGAACTTGCAATTGCCGCGCGCATTGCGGCGC 50 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACTTCACTTAATTTAGTGTCAATCTGTTTGAAGCTAGCAGAAATGAACCA 100 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CATCATCCAGGACAAGTACATTCCGCAACAGCTGCTCTACTTCTTGGCGG 150 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GACGGCGCTGCTGCCAGCAGTTCCCGTGCACATTCCGCACCAGCGAGCAC 200 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GAAGCATTCGCCAACAACGCCCGCTCCTTGGGGCTGCGATCGCAGGTGGT 250 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCATGTCAACGGGAATAGCTGCGTGAAGGTGTACAAGCAGGCATGTCGCC 300 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACTACCTGGAGGAGCCCAAGACGCTGGTTTTGTCCTCGGGCGCTACGCTG 350 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AACATGTTTACGCTGCTGAGCAGGAAGTCCTTGATGGGCAAGGAGGATTT 400 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GGAGCTTTACGCGGACCTCGTCTCCATGAAGGCCAATGCCTCGGACCTAC 450 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGTCACTGCATCTGCCGCTGCCCGCCATACGTCCTCCTAACCTGCGCTTC 500 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGGACGGAGGCGCAGCTGAATTTCCTGACTGCTTTTCTGGGTCACTCGCT 550 *F PNBP_28356-28844 *F \---------------------------------------------TCGCT 5 *F gi|9651990|gb|AF255662.1|AF255 *F GAGCGACGAGATCTTGCAGTCATTGTACGCCAGTCGGGTGATCGTCTATA 600 *F PNBP_28356-28844 *F GAGCGACGAGATCTTGCAGTCATTGTACGCCAGTCGGGTGATCGTCTATA 55 *F gi|9651990|gb|AF255662.1|AF255 *F ACGCTGCGCTTTGCTGGGACAAGTCCGTCTTCCTGCCCCTGGTCATTCTG 650 *F PNBP_28356-28844 *F ACGCTGCGCTTTGCTGGGACAAGTCCGTCTTCCTGCCCCTGGTCATTCTG 105 *F gi|9651990|gb|AF255662.1|AF255 *F GACGATTGCCGGAACAAGAAGTCCAACGTCAAGATTATGTGCATCGAGCG 700 *F PNBP_28356-28844 *F GACGATTGCCGGAACAAGAAGTCCAACGTCAAGATTATGTGCATCGAGCG 155 *F gi|9651990|gb|AF255662.1|AF255 *F GCAGGCCATTCTGGCCACCTACAACAGCCAGCGAACGGCCAACTTCTTCG 750 *F PNBP_28356-28844 *F GCAGGCCATTCTGGCCACCTACAACAGCCAGCGAACGGCCAACTTCTTCG 205 *F gi|9651990|gb|AF255662.1|AF255 *F GCGAGCAGCTGGGCGAGACGGTGGGCATCCAGTTGCCGTACTTCAGCGCC 800 *F PNBP_28356-28844 *F GCGAGCAGCTGGGCGAGACGGTGGGCATCCAGTTGCCGTACTTCAGCGCC 255 *F gi|9651990|gb|AF255662.1|AF255 *F GTTAGCTCCAGCACTTTTATTATTTACTCGACGGCGCAGTACTTTTTGCG 850 *F PNBP_28356-28844 *F GTTAGCTCCAGCACTTTTATTATTTACTCGACGGCGCAGTACTTTTTGCG 305 *F gi|9651990|gb|AF255662.1|AF255 *F CTCGCTGACCAGCCAGCAGTTTCGCAACATCAGTCACCTGGTGGTGAACG 900 *F PNBP_28356-28844 *F CTCGCTGACCAGCCAGCAGTTTCGCAACATCAGTCACCTGGTGGTGAACG 355 *F gi|9651990|gb|AF255662.1|AF255 *F ATGTCCACCTGCATGATCCCTACACCGACATCCTGTTGAGCGAGATACGC 950 *F PNBP_28356-28844 *F ATGTCCACCTGCATGATCCCTACACCGACATCCTGTTGAGCGAGATACGC 405 *F gi|9651990|gb|AF255662.1|AF255 *F ATGGCGCTAAGTAGCCATCAGAACCTGAGGGTGGTGCTGCTGTCGCAAAT 1000 *F PNBP_28356-28844 *F ATGGCGCTAAGTAGCCATCAGAACCTGAGGGTGGTGCTGCTGTCGCAAAT 455 *F gi|9651990|gb|AF255662.1|AF255 *F GGGAAACCCCAAGAAGTTCACCGACTTCTTTGGCGAGGGATTGCAACTGA 1050 *F PNBP_28356-28844 *F GGGAAACCCCAAGAAGTTCACCGACTTCTTTGGC---------------- 489 *F gi|9651990|gb|AF255662.1|AF255 *F ATATGATTAAGCAGCCGGAGGTCGCACCACGTGTCTCCTACTTGAACGAG 1100 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTGCACAGCTGCATTGCCCTGGCCGGCATCCACAAGGGTCCGGACATCTA 1150 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAAGGAGATTCCGGAGGCTTTTCGCGCCAACAATCCGCGCAACGAGCAGA 1200 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGGACAAGTGCCTGCAGGCCTACGGAGAGTTGGGCACGGACGCCGCCCTA 1250 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGTCCCTTCCTGTACGCCGTAAACTACGACCTGGCTCCCGTAAACTACCG 1300 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCACTCGCTGACCGGCAAAACGGCGGTGCACTTCGCCTCCGAACTGAACA 1350 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AGGCCAATCATCTGCGCCTGCTGCTCTTCATGGGCGCTGATCCCTACATT 1400 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GTGGACCTGTTCCAGCAGAACGCCATCTCACTGGCGGCCATGAACGGCAA 1450 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCACGAGTGCATCGATGTCCTGAACAGCTACAGTCTGCACGGCTATGTGG 1500 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGAAGAGCGCCAAGCCGGATTTCGTCGACTATGATCTTATTATCGACATC 1550 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATGTATCTGCTGCGCACCAAACCGGAGTATTCGCCAGGTGAGTATTCGCC 1600 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AGGCAACATCCTCATCATTTTACCCACCTACTACCACATTGTCAAGCTGA 1650 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACTACATGATCCTGAGCCACTGCCTCACTGGCAGCCTGCAGGAGTGCTCC 1700 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATATTCCTGCTGTACGATAACATGAGGAATGACTACCTCCAGGCGTTGGT 1750 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAATGCCAGCGATGAGACGGTGAAGGTGGTTCTGGCCACGGATATTATCG 1800 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AATCGCTCTGCCTGAAGGTGCCGTTCAAGTACCAAATAGACACCGCCTGC 1850 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGGCTGAACAATGTTTACGATACCACGAGCTGCAGCGGCGATGATCGCTT 1900 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGAATGGGTGGCCAAGGACGCTCTTCTGCGAAGGGAGCTAATCCTTCAGC 1950 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCAATAAAGGGGATGTACAATGCTTCCGTCTGATAAGCAAGGAGGCCTAC 2000 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GAGGAGCTAAGCGAGACCAGTCAGCCAAGCCTGCAGACTATGCAACTGGA 2050 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAAGATCTGCCTGGCGGTGAAACTACTCTCACCCAATACGATTATAAGCG 2100 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AATATCTGGGTATTACCATCTCACCGCCACCGCTGATCAACGTGCACCAT 2150 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCCGTGCAGTTCCTCAAGAAAATAGATGTGCTCGATGACGCCGAGGATGT 2200 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GACCTGGCTGGGCTGCCGCCTGATGGACATCCCGGTCTCCTGTCAACTCG 2250 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCCGCATGCTGATCTTCGGCATCCTGCTGCGCTGCCTGGATCCAATACTC 2300 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACGATTGTCAGCTCTCTGTCGACGGCGGACCCGTTGGGCATTCCGTTTAC 2350 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGAGGACATCGACAACTTGTGGGACAGGTTCACCATCTACATCCAGAACA 2400 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCATCAAGAAAGAGCGGACCTACCTGTCCGACAATCAGTTTTCCGATCAC 2450 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TTCATCTTCGTGCGCTTGTACAAGGAGTGGCAAAACCGAATGCACAACAG 2500 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GACGCCGCCGCTATACCTTAAGGATGAGTATGAATTCATGCTGAATGGCC 2550 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGATGGAGCAACTCACCTCCATTCGTTCCGAGATCGTGAGCTCCTTGCGG 2600 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCAGCCAATTTGATTCACAGCCGAGGTAAGCTGTCGATGAACAATCTCAA 2650 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCAGATGTCCTGCAATTGGCACATGGTTAAGGCGGCCCTCACGGGCGGAA 2700 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGTATCCCAACATCTATGCCGTGGACACTAGAAAGAGTTCGCTAAAGTCG 2750 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCCTTCTCCGGGAACGTTTCCATGCATCCGAACACCGTGCTCCGCGATTT 2800 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTTGGAGCCACTGAATATTTCGGCACAGAGCTTCCGCACACCGTGGATTG 2850 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGTGCAACAGGCAGAAGAGCCACATTGTTTACGCCACCCTGGTCGTTCCT 2900 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTGGCTGTTGCCATGTTCTCGGGACATCCACGGATTCGCCTCTCCCCGAT 2950 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTGCGACAGTGAAATGAGTTTGACCGATCGCAACGTGAACGTCTTTATAG 3000 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACGAATGGATCTGGATGGTTATGTCCAAGGCTACGGCGGAGATGGTGATG 3050 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGCACGCGGTATTACTTCTTTAAAATGTACCACGACTTGCTGAAGCACTG 3100 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAGCGAGCTGGACATGTGGCGCCGAGACTGCGAGCCAGTTTCCCAATACA 3150 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCGTGCTGACTGATACGCTATCGAAGATCTTCGAGAGTGAGGATGGATTC 3200 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GTGGGCTTCTTCAAGCCTCCGCCCATAACCTTTCTACCGACGCCCCAGCT 3250 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCCATCCTTGTACCTCCTGTCCGTTAACGCCCACTTCAGTTGGGCCCGGG 3300 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AAGTGGAGGAGAACATGCTGTCGAAACCACACCATTTCAATTCGCACTTC 3350 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATCGAGAGGCAGTTCTTCGTGCTCTACGCAGGCGGCGACTGCGAGGAGTT 3400 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCACAGCAGGAACACGCCAGCCTTTATTGAAAGTGTCCTCGGCAAGTTTG 3450 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TCAGACCCATCGACACGCCCAACCGGCACATCTTTGTGATCCTGTATAGA 3500 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AAGGATCCCGATATGATGCTCAGCATTAGTCGAGCCAAATTCGTCAATGG 3550 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGTATTTATGTTGCAGGAATACTTCCGAAACAACATTCCGGTGTTTGAAA 3600 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TCTTAGATGCGTGTGTGTCGTTGAATGTGCAGACACCGGTGTTTGATGGC 3650 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGCCTGATGTCGGCGCTAATTGACAAGCGTGTCGGCAACTTGATTATGGA 3700 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATTGTTTGCCTTCCGCCACCACTGGATTCACAAGCGATAAAGCCCGCTTC 3750 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTCGCGCATATTGCCTGCTTCCCTACTAATTGAGTCCTAAAATAATTAAG 3800 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AATGATCCAAAGAAGCGTGCCGAAGTCCAAATAGGACTTGATTGTGCCTT 3850 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TTATTGCTGTGAAATGACTGTTAATTTTAAATGAGAGCTAACCAGAATCA 3900 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AAATACGTACTTTTAGTACCACATTGCGTCTATCATCGTTGTTTTGAAAT 3950 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATTTGAAATTTGTGCATCCCAATCATGAGTAGATGTGAGACAATAAAGAA 4000 *F PNBP_28356-28844 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 GAAGTATCTTTTAATACTAGC 4021 *F PNBP_28356-28844 \--------------------- *F \------------------------------------ *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: gi|9651990|gb|AF255662.1|AF255 4021 bp *F Sequence 2: PNBP_31159-31671 513 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 100 *F Guide tree file created: *F [/net/nfs0/vol1/production/w3nobody/tmp/981553.746551.dnd] *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:9747 *F Alignment Score 3839 *F CLUSTAL-Alignment file created *F [/net/nfs0/vol1/production/w3nobody/tmp/981553.746551.aln] *F Your guide tree: *F 981553.746551.dnd *F (gi|9651990|gb|AF255662.1|AF255:0.00000,PNBP_31159-31671:0.00000); *F Your Multiple Sequence Alignment: *F 981553.746551.aln *F CLUSTAL W (1.81) multiple sequence alignment *F gi|9651990|gb|AF255662.1|AF255 *F CACGACGGTGGCAATAACGGAACTTGCAATTGCCGCGCGCATTGCGGCGC 50 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACTTCACTTAATTTAGTGTCAATCTGTTTGAAGCTAGCAGAAATGAACCA 100 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CATCATCCAGGACAAGTACATTCCGCAACAGCTGCTCTACTTCTTGGCGG 150 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GACGGCGCTGCTGCCAGCAGTTCCCGTGCACATTCCGCACCAGCGAGCAC 200 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GAAGCATTCGCCAACAACGCCCGCTCCTTGGGGCTGCGATCGCAGGTGGT 250 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCATGTCAACGGGAATAGCTGCGTGAAGGTGTACAAGCAGGCATGTCGCC 300 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACTACCTGGAGGAGCCCAAGACGCTGGTTTTGTCCTCGGGCGCTACGCTG 350 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AACATGTTTACGCTGCTGAGCAGGAAGTCCTTGATGGGCAAGGAGGATTT 400 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GGAGCTTTACGCGGACCTCGTCTCCATGAAGGCCAATGCCTCGGACCTAC 450 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGTCACTGCATCTGCCGCTGCCCGCCATACGTCCTCCTAACCTGCGCTTC 500 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGGACGGAGGCGCAGCTGAATTTCCTGACTGCTTTTCTGGGTCACTCGCT 550 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GAGCGACGAGATCTTGCAGTCATTGTACGCCAGTCGGGTGATCGTCTATA 600 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACGCTGCGCTTTGCTGGGACAAGTCCGTCTTCCTGCCCCTGGTCATTCTG 650 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GACGATTGCCGGAACAAGAAGTCCAACGTCAAGATTATGTGCATCGAGCG 700 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCAGGCCATTCTGGCCACCTACAACAGCCAGCGAACGGCCAACTTCTTCG 750 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCGAGCAGCTGGGCGAGACGGTGGGCATCCAGTTGCCGTACTTCAGCGCC 800 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GTTAGCTCCAGCACTTTTATTATTTACTCGACGGCGCAGTACTTTTTGCG 850 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTCGCTGACCAGCCAGCAGTTTCGCAACATCAGTCACCTGGTGGTGAACG 900 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATGTCCACCTGCATGATCCCTACACCGACATCCTGTTGAGCGAGATACGC 950 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATGGCGCTAAGTAGCCATCAGAACCTGAGGGTGGTGCTGCTGTCGCAAAT 1000 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GGGAAACCCCAAGAAGTTCACCGACTTCTTTGGCGAGGGATTGCAACTGA 1050 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATATGATTAAGCAGCCGGAGGTCGCACCACGTGTCTCCTACTTGAACGAG 1100 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTGCACAGCTGCATTGCCCTGGCCGGCATCCACAAGGGTCCGGACATCTA 1150 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAAGGAGATTCCGGAGGCTTTTCGCGCCAACAATCCGCGCAACGAGCAGA 1200 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGGACAAGTGCCTGCAGGCCTACGGAGAGTTGGGCACGGACGCCGCCCTA 1250 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGTCCCTTCCTGTACGCCGTAAACTACGACCTGGCTCCCGTAAACTACCG 1300 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCACTCGCTGACCGGCAAAACGGCGGTGCACTTCGCCTCCGAACTGAACA 1350 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AGGCCAATCATCTGCGCCTGCTGCTCTTCATGGGCGCTGATCCCTACATT 1400 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GTGGACCTGTTCCAGCAGAACGCCATCTCACTGGCGGCCATGAACGGCAA 1450 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCACGAGTGCATCGATGTCCTGAACAGCTACAGTCTGCACGGCTATGTGG 1500 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGAAGAGCGCCAAGCCGGATTTCGTCGACTATGATCTTATTATCGACATC 1550 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATGTATCTGCTGCGCACCAAACCGGAGTATTCGCCAGGTGAGTATTCGCC 1600 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AGGCAACATCCTCATCATTTTACCCACCTACTACCACATTGTCAAGCTGA 1650 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACTACATGATCCTGAGCCACTGCCTCACTGGCAGCCTGCAGGAGTGCTCC 1700 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATATTCCTGCTGTACGATAACATGAGGAATGACTACCTCCAGGCGTTGGT 1750 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAATGCCAGCGATGAGACGGTGAAGGTGGTTCTGGCCACGGATATTATCG 1800 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AATCGCTCTGCCTGAAGGTGCCGTTCAAGTACCAAATAGACACCGCCTGC 1850 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGGCTGAACAATGTTTACGATACCACGAGCTGCAGCGGCGATGATCGCTT 1900 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGAATGGGTGGCCAAGGACGCTCTTCTGCGAAGGGAGCTAATCCTTCAGC 1950 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCAATAAAGGGGATGTACAATGCTTCCGTCTGATAAGCAAGGAGGCCTAC 2000 *F PNBP_31159-31671 *F \--------------------TGCTTCCGTCTGATAAGCAAGGAGGCCTAC 30 *F gi|9651990|gb|AF255662.1|AF255 *F GAGGAGCTAAGCGAGACCAGTCAGCCAAGCCTGCAGACTATGCAACTGGA 2050 *F PNBP_31159-31671 *F GAGGAGCTAAGCGAGACCAGTCAGCCAAGCCTGCAGACTATGCAACTGGA 80 *F gi|9651990|gb|AF255662.1|AF255 *F CAAGATCTGCCTGGCGGTGAAACTACTCTCACCCAATACGATTATAAGCG 2100 *F PNBP_31159-31671 *F CAAGATCTGCCTGGCGGTGAAACTACTCTCACCCAATACGATTATAAGCG 130 *F gi|9651990|gb|AF255662.1|AF255 *F AATATCTGGGTATTACCATCTCACCGCCACCGCTGATCAACGTGCACCAT 2150 *F PNBP_31159-31671 *F AATATCTGGGTATTACCATCTCACCGCCACCGCTGATCAACGTGCACCAT 180 *F gi|9651990|gb|AF255662.1|AF255 *F GCCGTGCAGTTCCTCAAGAAAATAGATGTGCTCGATGACGCCGAGGATGT 2200 *F PNBP_31159-31671 *F GCCGTGCAGTTCCTCAAGAAAATAGATGTGCTCGATGACGCCGAGGATGT 230 *F gi|9651990|gb|AF255662.1|AF255 *F GACCTGGCTGGGCTGCCGCCTGATGGACATCCCGGTCTCCTGTCAACTCG 2250 *F PNBP_31159-31671 *F GACCTGGCTGGGCTGCCGCCTGATGGACATCCCGGTCTCCTGTCAACTCG 280 *F gi|9651990|gb|AF255662.1|AF255 *F GCCGCATGCTGATCTTCGGCATCCTGCTGCGCTGCCTGGATCCAATACTC 2300 *F PNBP_31159-31671 *F GCCGCATGCTGATCTTCGGCATCCTGCTGCGCTGCCTGGATCCAATACTC 330 *F gi|9651990|gb|AF255662.1|AF255 *F ACGATTGTCAGCTCTCTGTCGACGGCGGACCCGTTGGGCATTCCGTTTAC 2350 *F PNBP_31159-31671 *F ACGATTGTCAGCTCTCTGTCGACGGCGGACCCGTTGGGCATTCCGTTTAC 380 *F gi|9651990|gb|AF255662.1|AF255 *F CGAGGACATCGACAACTTGTGGGACAGGTTCACCATCTACATCCAGAACA 2400 *F PNBP_31159-31671 *F CGAGGACATCGACAACTTGTGGGACAGGTTCACCATCTACATCCAGAACA 430 *F gi|9651990|gb|AF255662.1|AF255 *F GCATCAAGAAAGAGCGGACCTACCTGTCCGACAATCAGTTTTCCGATCAC 2450 *F PNBP_31159-31671 *F GCATCAAGAAAGAGCGGACCTACCTGTCCGACAATCAGTTTTCCGATCAC 480 *F gi|9651990|gb|AF255662.1|AF255 *F TTCATCTTCGTGCGCTTGTACAAGGAGTGGCAAAACCGAATGCACAACAG 2500 *F PNBP_31159-31671 *F TTCATCTTCGTGCGCTTGTACAAGGAGTGGCAA----------------- 513 *F gi|9651990|gb|AF255662.1|AF255 *F GACGCCGCCGCTATACCTTAAGGATGAGTATGAATTCATGCTGAATGGCC 2550 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGATGGAGCAACTCACCTCCATTCGTTCCGAGATCGTGAGCTCCTTGCGG 2600 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCAGCCAATTTGATTCACAGCCGAGGTAAGCTGTCGATGAACAATCTCAA 2650 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCAGATGTCCTGCAATTGGCACATGGTTAAGGCGGCCCTCACGGGCGGAA 2700 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGTATCCCAACATCTATGCCGTGGACACTAGAAAGAGTTCGCTAAAGTCG 2750 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCCTTCTCCGGGAACGTTTCCATGCATCCGAACACCGTGCTCCGCGATTT 2800 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTTGGAGCCACTGAATATTTCGGCACAGAGCTTCCGCACACCGTGGATTG 2850 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGTGCAACAGGCAGAAGAGCCACATTGTTTACGCCACCCTGGTCGTTCCT 2900 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTGGCTGTTGCCATGTTCTCGGGACATCCACGGATTCGCCTCTCCCCGAT 2950 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTGCGACAGTGAAATGAGTTTGACCGATCGCAACGTGAACGTCTTTATAG 3000 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ACGAATGGATCTGGATGGTTATGTCCAAGGCTACGGCGGAGATGGTGATG 3050 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGCACGCGGTATTACTTCTTTAAAATGTACCACGACTTGCTGAAGCACTG 3100 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CAGCGAGCTGGACATGTGGCGCCGAGACTGCGAGCCAGTTTCCCAATACA 3150 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCGTGCTGACTGATACGCTATCGAAGATCTTCGAGAGTGAGGATGGATTC 3200 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GTGGGCTTCTTCAAGCCTCCGCCCATAACCTTTCTACCGACGCCCCAGCT 3250 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F GCCATCCTTGTACCTCCTGTCCGTTAACGCCCACTTCAGTTGGGCCCGGG 3300 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AAGTGGAGGAGAACATGCTGTCGAAACCACACCATTTCAATTCGCACTTC 3350 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATCGAGAGGCAGTTCTTCGTGCTCTACGCAGGCGGCGACTGCGAGGAGTT 3400 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CCACAGCAGGAACACGCCAGCCTTTATTGAAAGTGTCCTCGGCAAGTTTG 3450 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TCAGACCCATCGACACGCCCAACCGGCACATCTTTGTGATCCTGTATAGA 3500 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AAGGATCCCGATATGATGCTCAGCATTAGTCGAGCCAAATTCGTCAATGG 3550 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TGTATTTATGTTGCAGGAATACTTCCGAAACAACATTCCGGTGTTTGAAA 3600 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TCTTAGATGCGTGTGTGTCGTTGAATGTGCAGACACCGGTGTTTGATGGC 3650 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CGCCTGATGTCGGCGCTAATTGACAAGCGTGTCGGCAACTTGATTATGGA 3700 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATTGTTTGCCTTCCGCCACCACTGGATTCACAAGCGATAAAGCCCGCTTC 3750 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F CTCGCGCATATTGCCTGCTTCCCTACTAATTGAGTCCTAAAATAATTAAG 3800 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AATGATCCAAAGAAGCGTGCCGAAGTCCAAATAGGACTTGATTGTGCCTT 3850 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F TTATTGCTGTGAAATGACTGTTAATTTTAAATGAGAGCTAACCAGAATCA 3900 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F AAATACGTACTTTTAGTACCACATTGCGTCTATCATCGTTGTTTTGAAAT 3950 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 *F ATTTGAAATTTGTGCATCCCAATCATGAGTAGATGTGAGACAATAAAGAA 4000 *F PNBP_31159-31671 *F \-------------------------------------------------- *F gi|9651990|gb|AF255662.1|AF255 GAAGTATCTTTTAATACTAGC 4021 *F PNBP_31159-31671 \--------------------- *F \-------- # *U FBrf0131149 *a Yang *b M. *t 2000.10.10 *T personal communication to FlyBase *u *F From fbserver@ebi.ac.uk Tue Oct 10 11:37:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 10 Oct 2000 11:37:59 \+0100 *F Date: Tue, 10 Oct 2000 11:37:30 \+0100 (BST) *F From: fbmailer@bio.indiana.edu *F Reply-to: m.yang@gen.cam.ac.uk *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail *F Content-Length: 420 *F comments: Dear Sir, *F Could you add more information into gene ORF1 (CG7417). There is a *F P{GAL4} insertion line (P{GawB}201Y, stock No. 4440), which has been used by *F several labs. We found this P element was inserted into the first intron of *F this gene. *F Dr. M Yang *F mailto: flybase-help@morgan.harvard.edu *F realname: M Yang *F reply-to: m.yang@gen.cam.ac.uk *F Sent from computer cok-mac1.gen.cam.ac.uk # *U FBrf0131154 *a Romero *b M.F. *c P.J. *d Harte *t 2000.10.11 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Aug 24 12:35:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 24 Aug 2000 12:35:01 \+0100 *F To: mfr2@po.cwru.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 24 Aug 2000 12:41:37 \+0100 *F Content-Length: 1061 *F Dear Dr. Romero, *F I am curating your paper for FlyBase: *F Romero et al., 2000, J. Biol. Chem. 275(32): 24552--24559 *F about ndae1. *F Towards the end of the paper you describe a P-element line from the *F BDGP that is inserted in the 5' untranslated region of ndae1, but you *F do not give the identifier number of this line, e.g. k12345. *F I would be grateful if you could tell me the insertion number of the *F P-element line, as this would enable me to merge in the record for that *F particular lethal insertion with the ndae1 gene record. *F The information would be recorded in FlyBase as a personal *F communication from you to FlyBase, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From mfr2@po.cwru.edu Wed Oct 11 17:31:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 11 Oct 2000 17:31:26 \+0100 *F X-Sender: mfr2@pop.cwru.edu *F X-Mailer: Windows Eudora Light Version 3.0.1 (32) *F Date: Wed, 11 Oct 2000 12:30:04 \-0400 *F To: Gillian Millburn (Genetics) *F From: 'Michael F. Romero, PhD' *F Subject: Re: FlyBase query *F Cc: pjh3@po.cwru.edu *F Mime-Version: 1.0 *F Dear Gillian, *F . *F . *F The P element strain we have is l(2)k10316. *F . *F . *F Sincerely, michael *F Michael F. Romero, PhD *F Dept. Physiology & Biophysics, Rm \#SOM-E563 *F Case Western Reserve University, School of Medicine, 2119 Abington Rd *F Cleveland, OH 44106-4970 *F office: 216-368-3180; lab: 216-368-3912; fax: 216-368-3952 *F email: mfr2@po.cwru.edu *F NEW WEB-site ==> http://physiology.cwru.edu *F http://physiology.cwru.edu/faculty/staff_electro/romero_main.html *F http://pharmacology.cwru.edu/Faculty/romero.htm *F http://expertise.cos.com/cgi-bin/exp.cgi?id=675455 *F check out our DPB Monday Seminars ==> *F http://physiology.cwru.edu/calendar.html *F '... and now for something completely different!....' Monty Python, 'The *F Flying Circus' # *U FBrf0131155 *a Whitfield *b E. *t 2000.8.29 *T personal communication to FlyBase *u FlyBase error report for CG17263 on Tue Aug 29 03:21:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Aug 29 11:14:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 29 Aug 2000 11:14:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 29 Aug 2000 03:21:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17263 on Tue Aug 29 03:21:10 2000 *F Content-Length: 859 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17263 *F Gene annotation error *F Gene CG17263 corresponds to FBgn0005612 *F Comments: Alignment of CG17263 to Sox14 protein sequence demonstrates these *F are the same *F genes (Celera failed to define C terminus of Sox14 due to missed splice sites). *F Q=Sox14, 530 aa long, protein sequence from FB annotated ref sequence page *F (http://fly.ebi.ac.uk:7081/.data/maps/sean/gifs/FBgn0005612.html#hit4) *F T=TrEMBL entry Q9W1E3 corresponding to CG17263 PROTEIN. *F Q 1 MIAKPNQATTEPPLSLRPGTVPTVPATTPARPATITIQRRHPAPKADSTPHTLPPFSPSP *F T 1 MIAKPNQATTEPPLSLRPGTVPTVPATTPARPATITIQRRHPAPKADSTPHTLPPFSPSP *F Q 61 SPASSPSPAPAQTPGAQKTQSQAAITHPAAVASPSAPV *F T 61 SPASSPSPAPAQTPGAQKTQSQAAITHPAAVASPSAPL *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131156 *a Lemaitre *b B. *t 2000.9.1 *T personal communication to FlyBase *u FlyBase error report for CG1857 on Thu Aug 31 23:59:09 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Sep 01 07:52:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 1 Sep 2000 07:52:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 31 Aug 2000 23:59:09 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: lemaitre@cgm.cnrs-gif.fr *F Subject: FlyBase error report for CG1857 on Thu Aug 31 23:59:09 2000 *F Content-Length: 478 *F Error report from Bruno Lemaitre (lemaitre@cgm.cnrs-gif.fr) *F Gene or accession: CG1857 *F Gene annotation error *F Comments: CG1857 correspond to one gene of the necrotic locus (spn43AC: *F flybase number FBgn0028676 ). *F The information on this gene is given in FBgn0002930 under the name necrotic. *F it exists some mutation of this gene (not mentioned in FBgn0028676 but in *F FBgn0002930) *F Browser: Mozilla/3.01 [fr] (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131157 *a Whitfield *b E. *t 2000.10.19 *T personal communication to FlyBase *u FlyBase error report for CG11895 on Thu Oct 19 04:19:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 19 12:19:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 19 Oct 2000 12:19:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 19 Oct 2000 04:19:43 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11895 on Thu Oct 19 04:19:42 2000 *F Content-Length: 556 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11895 *F Gene annotation error *F Gene CG11895 has incorrect exon/intron structure. *F Comments: Celera define the wrong C terminal exon for stan (AE003828; *F AAF58763) in the *F latest release of the sequence. *F The correct sequence is shown in Chae et al, Development 126:5421-5429(1999) *F (AF172329; AAF02618). *F Please update the CDS and mRNA features, the DNA is there for correct *F translation. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131158 *a Whitfield *b E. *t 2000.10.19 *T personal communication to FlyBase *u FlyBase error report for CG2140 on Thu Oct 19 04:36:24 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 19 12:36:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 19 Oct 2000 12:36:26 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 19 Oct 2000 04:36:24 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2140 on Thu Oct 19 04:36:24 2000 *F Content-Length: 769 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2140 *F Gene annotation error *F Gene CG2140 has incorrect exon/intron structure. *F Comments: The new release of Celera sequence has converted Cyt-b5 from a *F beautiful 134aa *F protein translation that matches so well with the same protein from other *F species into a 40aa N terminal truncation. *F Any chance you could update the CDS and mRNA feature to revert to the previous *F translation? *F AE003840; AAF59233 *F The old base span was join(complement(242697..242816), complement(240811.. *F 241095) but now you have reversed the DNA so Cyt-b5 reads on the other strand *F I don't know the positions. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131159 *a Whitfield *b E. *t 2000.10.20 *T personal communication to FlyBase *u FlyBase error report for CG3630 on Fri Oct 20 03:02:22 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Oct 20 11:02:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 20 Oct 2000 11:02:24 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 20 Oct 2000 03:02:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3630 on Fri Oct 20 03:02:22 2000 *F Content-Length: 635 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3630 *F Gene annotation error *F Gene CG3630 has incorrect exon/intron structure. *F Comments: The new translation of EG:152A3.3 (AE003423; AAF45738.2) no longer *F matches *F the prediction from EDGP (AL009194; CAA15697). Previously it was a 100% match *F so please update the mRNA and CDS features to revert back to the original *F translation. *F CDS join(101715..101759,104300..105454) *F change to *F CDS join(101715..101759,104315..104611,104681..105454) *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131160 *a Whitfield *b E. *t 2000.10.20 *T personal communication to FlyBase *u FlyBase error report for CG17332 on Fri Oct 20 05:01:54 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Oct 20 13:01:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 20 Oct 2000 13:01:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 20 Oct 2000 05:01:54 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17332 on Fri Oct 20 05:01:54 2000 *F Content-Length: 801 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17332 *F Gene annotation error *F Gene CG17332 has incorrect exon/intron structure. *F Comments: The new Celera release has two translations of VhaSFD that are *F annotated as *F splice variants, they are not splice variants but instead mistakes in the *F definition of the splice sites. *F DR EMBL; AE003652; AAF53555.2 *F is correct and should be kept *F DR EMBL; AE003652; AAF53556.2 *F is wrong at positions (111709, 111609 and 108354 (and therefore also the *F position of the stop codon. *F Please delete this second CDS as there is no published evidence of a splice *F variant and the translations also do not show one (no extra or deleted exons). *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131161 *a Morcillo *b P. *t 2000.9.28 *T personal communication to FlyBase *u FlyBase error report for CG4727 on Thu Sep 28 09:03:16 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Sep 28 17:03:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 28 Sep 2000 17:03:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 28 Sep 2000 09:03:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: p-morcillo@ski.mskcc.org *F Subject: FlyBase error report for CG4727 on Thu Sep 28 09:03:16 2000 *F Content-Length: 696 *F Error report from Patrick Morcillo (p-morcillo@ski.mskcc.org) *F Gene or accession: CG4727 *F Gene annotation error *F Genes CG4727 and CG4760 should be merged. *F Comments: The published sequence of boule (bol,FBgn0011206, CG4760) cDNA *F (U51858) overlaps CG4727. Furthermore, there is a P-element which has the bol *F phenotype inserted in the 5' region of CG4727 which is male sterile, and is *F annotated in the CG database. For this reason I believe that the two genes are *F the same. *F You all have done a great job in putting everything together. *F Please feel free to contact me if you need more information. *F Patrick *F Browser: Mozilla/4.72 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131162 *a Imler *b J.L. *t 2000.10.17 *T personal communication to FlyBase *u FlyBase error report for CG7250 on Tue Oct 17 07:07:38 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 17 15:07:48 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Oct 2000 15:07:48 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 17 Oct 2000 07:07:38 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: JL.Imler@ibmc.u-strasbg.fr *F Subject: FlyBase error report for CG7250 on Tue Oct 17 07:07:38 2000 *F Content-Length: 789 *F Error report from Jean-Luc Imler (JL.Imler@ibmc.u-strasbg.fr) *F Gene or accession: CG7250 *F Missed gene *F Comments: We have studied the role of Toll-related molecules in the regulation *F of antimicrobial peptide gene expression. Our paper (S. Tauszig et al. 2000, *F PNAS 97: 10520-10525) compares the sequence of the 9 Toll molecules encoded by *F the Drosophila genome, their capacity to activate reporter genes and reports *F their expression patterns. In this paper, as well as in two recent review *F articles (S.A. Wasserman, 2000 Curr Opin Gent Dev, 10: 497-502; R.S. Khush and *F B. Lemaitre 2000 TIG 16: 442-449), CG7250 is called Toll-6. The Genbank *F accession number for the sequence is AF247766. *F Browser: Mozilla/4.04 [fr] (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131163 *a Imler *b J.L. *t 2000.10.17 *T personal communication to FlyBase *u FlyBase error report for CG1149 on Tue Oct 17 07:13:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 17 15:13:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Oct 2000 15:13:24 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 17 Oct 2000 07:13:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: JL.Imler@ibmc.u-strasbg.fr *F Subject: FlyBase error report for CG1149 on Tue Oct 17 07:13:17 2000 *F Content-Length: 796 *F Error report from Jean-Luc Imler (JL.Imler@ibmc.u-strasbg.fr) *F Gene or accession: CG1149 *F Missed gene *F Comments: We have studied the role of Toll-related molecules in the regulation *F of antimicrobial peptide gene expression. Our paper (S. Tauszig et al. 2000, *F PNAS 97: 10520-10525) compares the sequence of the 9 Toll molecules encoded by *F the Drosophila genome, their capacity to activate reporter genes and reports *F their expression patterns. In this paper, as well as in two recent review *F articles (S.A. Wasserman, 2000 Curr Opin Gent Dev, 10: 497-502; R.S. Khush and *F B. Lemaitre 2000 TIG 16: 442-449), CG1149 is called Toll-3. The Genbank *F accession number for the incomplete cDNA is AF247769. *F Browser: Mozilla/4.04 [fr] (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131164 *a Whitfield *b E. *t 2000.10.23 *T personal communication to FlyBase *u FlyBase error report for CG12846 on Mon Oct 23 06:06:10 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Oct 23 14:06:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 23 Oct 2000 14:06:16 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 23 Oct 2000 06:06:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12846 on Mon Oct 23 06:06:10 2000 *F Content-Length: 498 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12846 *F Gene annotation error *F Gene CG12846 has incorrect exon/intron structure. *F Comments: Translation of BcDNA:GH07074 has been changed in the new release *F (AE003842; *F AAF59312) so that it no longer matches the cDNA translation from the BDGP group *F (AF220042; AAF23826). *F Please revert to the translation provided in release 1. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131165 *a Whitfield *b E. *t 2000.10.23 *T personal communication to FlyBase *u FlyBase error report for CG18104 on Mon Oct 23 04:55:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Oct 23 12:55:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 23 Oct 2000 12:55:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 23 Oct 2000 04:55:02 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18104 on Mon Oct 23 04:55:02 2000 *F Content-Length: 708 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18104 *F Gene annotation error *F Gene CG18104 has incorrect exon/intron structure. *F Comments: Predicted translation for arginase (AE003417; AAF45516.2) in the new *F release *F contains too many exons. *F Edit: *F FT CDS join(273612..273816,274010..274213,274275..274461, *F FT 290611..290710,291668..292027) *F to: *F FT CDS join(273612..273816,274010..274213,274275..274465) *F and you will have sequence that is a 100% match to that from Samson, submitted *F (FEB-1998) to the EMBL databases (AF047180; AAC03552). *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131166 *a Wehrli *b M. *t 2000.10.22 *T personal communication to FlyBase *u FlyBase error report for CG5912 on Sun Oct 22 08:14:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Oct 22 16:15:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 22 Oct 2000 16:15:03 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 22 Oct 2000 08:14:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mwehrli@mail.med.upenn.edu *F Subject: FlyBase error report for CG5912 on Sun Oct 22 08:14:57 2000 *F Content-Length: 2191 *F Error report from Marcel Wehrli (mwehrli@mail.med.upenn.edu) *F Gene or accession: CG5912 *F cDNA or EST error *F Comments: We have isolated two cDNA clones for the arrow gene where one is *F only 2nt shorter at the 5' end. A comparison with CG5912 shows that your *F sequence misses a 5' coding exon and at least one 5' non-coding exon. The ORF *F sequence missing encodes a the signal peptide and is separated from the bulk *F of the ORF by a 22,251 bp intron [coding sequence missing in exon3 i.e. *F immediately 5' to CG5912 is 'TTCAC']. The total of our additional 5' sequence is *F AGCACACTGTTCGAAAGAGCGAGACGAAAAACACGGCAACACGGCGGACGGAAAACTCAGTGAAAATTTTTCCGTGTGAT *F TATCATGTGCGCATAACAAAAGACAAACACGATCGGGTCGCGTCATCTGTTGTTTTTGCGCCAAATGTCTTAAAATTCCG *F GAGATAGTGCTTCCAAAATAAGATGGCTCTCGAGCCATACACAAAGTCTGCTGAAATCGAACCTCAATTGTGCCAAGTTA *F TAGATAATAATGAAGATGTGACAACGAAAGCGACGAAATGCGTGAAATCTAACAGCGGAGTGGGCTGGCGACAAATGCTG *F ATCGGCTTTCTACTGATTTGCTTTGGCATCTCAAACAGTTGGCAGTACAAAAATGTTCAC *F and the 3' UTR *F CATTTTGAGAGTTACATGTAAATTGAGAGGAGGAGCAACATATCACTTCTTTGCAAAGCTTTTCGTTTTATGAACTTTTT *F TTTTATTATTTTCCCAATTCTAATTGCTACAACTGATTTGTAAATATGTATGTGAGTTTAATTGGTCATTTAAGGCTTTA *F ACTAATTTTAATCATAGCTTAGAGAGAGCGTCCATGCCTGAAGGACTTATTCGATCTCGTTAAGTTTcATTTGCCAGATA *F GCCCGAAATAGATTGCAGTACAATGCTTTGCAGCTACCATACGCAACAATAGACTGCAGATTATTAGCAATTACCTTAGG *F TATAAATGATGTAATATTCGATAGGTGAGAGTCTTTTGAAATTCCGTGTGCCAAATTTTCTGAAACCTGACAGAAAATTC *F ACAATTGGTTAAAACCTAAGTACGCAATTTTGATTTAAATGCATTTAACGCCTAGACTTAGTTTAATATTTAGTTTAAAA *F GTGTAAATAGATAACTATATTATGAAGCAATATTGACGAATATGCGACTAATTCAACTGTCTTCTTAATTGTTATAAACA *F CCAAAAAGTGCCTCATTAATACGTGTGTTTTTTGTTTAATCTTTAATCCTCCTATATCATTTATTCATAGATAATACAAA *F TGAATAGTTAGTTATTCATACGACGTAGGTCTTTGCAATAAACAAATTGAAGTAAATTCATATTTATAGTTAATTTTAGT *F GAACGTAAAATGTAACAAACAATATTTTTAATTGTAAAATGCAGTATTTTTAAAAATTAAATATTGACTTTT *F Our analysis (Nature 407, 527-530) shows that arrow not only encodes and LDL *F receptor related protein closely related to mammalian LRP5 & LRP but is also a *F novel compenent in the wingless pathway. Arrow functions in the cell that *F receives the Wingless signal and probably is a co-receptor for Wingless, *F together with Frizzleds. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131167 *a Wehrli *b M. *t 2000.10.22 *T personal communication to FlyBase *u FlyBase error report for CG5912 on Sun Oct 22 08:15:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Oct 22 16:15:32 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 22 Oct 2000 16:15:32 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 22 Oct 2000 08:15:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mwehrli@mail.med.upenn.edu *F Subject: FlyBase error report for CG5912 on Sun Oct 22 08:15:26 2000 *F Content-Length: 345 *F Error report from Marcel Wehrli (mwehrli@mail.med.upenn.edu) *F Gene or accession: CG5912 *F Gene annotation error *F P element l(2)k08131 hits gene CG5912. *F Comments: other corrections pertaining to sequence and function of CG5912 *F submitted on a separate form *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131168 *a Imler *b J.L. *t 2000.10.17 *T personal communication to FlyBase *u FlyBase error report for CG5528 on Tue Oct 17 07:23:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 17 15:23:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Oct 2000 15:23:45 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 17 Oct 2000 07:23:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: JL.Imler@ibmc.u-strasbg.fr *F Subject: FlyBase error report for CG5528 on Tue Oct 17 07:23:37 2000 *F Content-Length: 425 *F Error report from JL.Imler (JL.Imler@ibmc.u-strasbg.fr) *F Gene or accession: CG5528 *F Missed gene *F Comments: Gene CG5528 is referred to as Toll-9 in three recent publications *F (S. Tauszig et al. 2000, PNAS 97: 10520-10525; S.A. Wasserman, 2000 Curr Opin *F Gent Dev, 10: 497-502; R.S. Khush and B. Lemaitre 2000 TIG 16: 442-449) *F Browser: Mozilla/4.04 [fr] (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131169 *a Sardiello *b M. *c G. *d Tripoli *t 2000.10.19 *T personal communication to FlyBase *u FlyBase error report for CG6105 on Thu Oct 19 02:57:34 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 19 10:57:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 19 Oct 2000 10:57:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 19 Oct 2000 02:57:34 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: m.sardiello@biologia.uniba.it *F Subject: FlyBase error report for CG6105 on Thu Oct 19 02:57:34 2000 *F Content-Length: 1521 *F Error report from Marco Sardiello and Gaetano Tripoli *F (m.sardiello@biologia.uniba.it) *F Gene or accession: CG6105 *F Gene annotation error *F Gene CG6105 should be split. *F Comments: In the course of our studies, we came to the notion that the gene *F CG6105 is composed by only 2 exons. Our observation is based on several ESTs *F found in the database. *F We think that the first and the second exons of current CG6105 in reality *F belong to another gene, which has opposed orientation. This is confirmed by *F ESTs SD02991, SD10808, SD01264, SD03496, LP11386 and others; these are all *F 5prime, and diverge from CG6105 coding. *F Here is our cDNA sequence: *F cDNA: *F cgcacactgaataggtaagttttccgcacatttcgttgatttcctcttgtataattccaactgcttgcagaaccaaattg *F aaaagatggcgagtttggctaccaagggatcaggacttgtgaacaggctcctcacacaggcgaggccccaactggacgtg *F ttcctgaagtacgccaaggtggaactgacgcccccgacgcccgccgatattccggccattcgccaaggactgggcaacat *F catcaagggagccaagaccggcgcctacaagaacctcacggttcgcgaggcctggcttaacaccctggtgaccgccgagg *F tcatcttctggttctacatcggcgagtgcatcggcaagcgtcacattgtaggctacaatgtctaagcttactatagtctc *F cgcttggcagtcactggaatgggcaacgtaatccctaacagatgtgtatatttatatgtctgcgaacatttcgactctga *F ataaagtgaaatagtaatttaaaattccgaaaattttcgaaaaatacattgttttttgaaaaccgttagaacgtttgcgc *F gggatttgtgtaagctaaagatgaggtgatgtaaaaccaagtttggaattaaaagttgacgatatttaatgataaaaatt *F aaaaaaatatatgtaatgttatacatcaaatgtttatgaaacggtgtcgtaatcaaagaggctaatggttcagaaataca *F taatatacttagagcatttaaaagca *F START: 86 STOP: 385 *F Browser: Mozilla/4.08 (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131170 *a Sardiello *b M. *c G. *d Tripoli *t 2000.10.19 *T personal communication to FlyBase *u FlyBase error report for CG1746 on Thu Oct 19 00:47:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 19 08:47:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 19 Oct 2000 08:47:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 19 Oct 2000 00:47:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: m.sardiello@biologia.uniba.it *F Subject: FlyBase error report for CG1746 on Thu Oct 19 00:47:27 2000 *F Content-Length: 7919 *F Error report from Marco Sardiello and Gaetano Tripoli *F (m.sardiello@biologia.uniba.it) *F Gene or accession: CG1746 *F Missed gene *F Comments: In the course of our studies, we came to the notion that the gene *F CG1746 is composed by almost 4 exons. Our observation is based on several ESTs *F found in the database. *F Moreover, the first exon of current CG1746 (corresponding to our second exon) *F has seven nucleotides at 5' not to be found in any EST. *F By aligning to the ESTs the cDNA we propose, it seems to match completely. *F Below, we report our cDNA sequence and our annotations to the genomic DNA. *F Our nucleotide \#1 in the gene sequence corresponds to nucleotide \#88547 of *F AE003777; our nucleotide \#5796 corresponds to nucleotide \#82748. *F Nucleotides 84465..84468 are absent on ESTs. *F We noticed that the P element in line EP(3)3264 is inserted in CG1746, as *F shown below *F cDNA: *F gttgtgaagggttcgttcgaaagccgtgcgctagcttcaattttgtcgtaagcaaaacaaaaaggaaattcaaccgaaaa *F tgttcgtgtcgacagtctctcgcattgccccagttgccaggagcgccttcctcgccaactccaagcagtacttgcgacca *F ttgagcagcgccatcatcagccagagccagactttggccgctcagaacacaacccccgttgcattgctgccacagatcag *F gtcattccagacctcgccagtcacgcgtgacattgactcggccgccaaattcattggtgctggtgccgcaacagtcggtg *F tcgctggatccggtgctggtatcggaacagtattcggttccctcatcatcggctacgccaggaacccatcgctgaaacag *F cagctgttctcctacgccattctgggcttcgccctgtccgaggccatgggtctgttctgtttgatgatggccttcctgct *F gctgttcgccttctaagcgcgcctgctgcacatcgattgctcgtccttgtttcctgcgatcgggcgcgtgcgccagtcgc *F atgcgaacgagaatagcaaccaaacaacacttcaaacaaacaaacaacaacaacaaccagaacaagcaacacctacgttc *F gttttcgattattaatgataaaatgattgattgattgattgatagcaaacaagaacatcctgtacaactatctaattata *F aataccaacaacaaccacataatacatgtaattttgtttttaaaaaacatgcgagcgaggagaagaccacacctacttta *F agttgaacaaccgaaatatcaggaggaaggaggattggattcaatcggattggactagccaacgctcacgcccataccca *F gacgacagacattaaaattagattttacttaaagcaagcttccggctcaccgaaaacacaacccaatcgatggcccgaac *F ga *F Gene: *F e1:1370..1390 e2:1878..1983 e3:2982..3186 e4:3749..4374 *F ATG 1936 TAA 3910 *F EP(3)3264:1379^1380 *F atgcaattgagggctgcaacattttggggggcgtaactgggtttaactgacttcaaattagcatcagtattatatttact *F tacagcattctgtgcttcgattcagttgcattcgcagttgccgctgggtaattaaaacataatttaagccgacgtgacgt *F agacacaaacaccgacgtcgaacgacgcgtaacgctgcgtttgtggtggtgtgcgtgactgtggtgagtttgcacgtacc *F agggctgcactgcattctcgggctcttagcgagtgcaccctcctaactaatttaagtctagcagatacaccttcctatag *F aacattaaatattttgagaatacaaacttatatttcgatgaaaatatgccggccggctctagttatttactaaggcatta *F ttccataagaaattattacaagaaataaataaaaaattttatattgtttattgcgtatagaattatgaaaagatgccaga *F gggctctagtcgtttccttttgtatatatagttccgtaaaaattaatgaaaacaataaataataattaattatatattat *F attatatattatatataatataaaattacacttttatttcaatttcgggataggcatacttgaagcttagctaaggttta *F ttaaaatgttaccatatatataatttttacatatgaatttaatattttccaccccatcaaatatattttaaaaagacagg *F gaaacttagggcacgatgaatattattacaactgaagtctggttatattttatatacataatttcatcaactatattgat *F gataaaaatatatttcacaatacatatgtatgtagttaaataaaagaatagattatgtgcatgtaaaacacaaacgatta *F tacatacttatttggccctacacttgccaaattcattgaaaacttatctaatttatctaattatctaattatctaattta *F tctattgcatttgctggttgaaaaattttcagaatgacccgtttttatggaccgttcgccaaagattttattttattaat *F ttgtagtataaataaataaacaaaattagaaagacgcagtttcgtgggaaagggctatttaattgattctacgtatccac *F tactgttatggtcacactgcgcctattgtgcaagggcaacctcctattctagcaaagactcgctcctggggcgcgtctct *F gctgaaaatgtgtagccaggacttgcaccgtggattgggttctcaaccaaaatggtatttctggtactttccagtattaa *F atcgacctatcgtcctttttcccagctcccgctgacggccacacttagcaccccccaaaagtcagcgaaattgtcaaatt *F cgttttactgttgtgaagggttcgttcgaagtgagttgctttttccgggtttttccgtccgctggcggagcacccccctt *F atcagcacccctctgttgggtaacagccgtgcaaacgcctcagatagccaaaaaacgcggaattagtgaagaatcgtgtg *F aaatttcgccaattaaagtgcgaaacgtgtttgataatgcgccgccatcttgcggcacactcgcacacacacacgtgtgc *F cattctggttttgcagtggtgtgcgtgtgtgcctcgagtcgttgcgtcactggaaaagttagaaaatcggcaaattcgca *F gaggaaaagccagcaaatcggtttcaattgatgactgagcacagatcgagctgagagtcgagtgaaaatccggtgcgagg *F gcagattttccgcatgttacgtaacttttctgaggcggcgtgggacttgatacaatgtattcttgggacagaaagtcagc *F ccagaactaatcgatatctccgtctatatctttgcagagccgtgcgctagcttcaattttgtcgtaagcaaaacaaaaag *F gaaattcaaccgaaaatgttcgtgtcgacagtctctcgcattgccccagttgccaggagcgccgtaagtaccccttcccc *F atttttatgtccgaattgtgcgagataaagttaaaatgtgcaggaaattacataattatcggaaaagtgcattgattttc *F ccgatctaaccatttgaatgccgccctggcgtggtgaacttccaatggccgcggtgagaaaacacggacggcacacagtt *F gcataactttgaggttatgtgtgcgcccagtggaatctactaattaaatgcgagaaattgtgaattatcgctcagcatct *F gtgcgtagaatttagtgagttcttttatttgcagtttcaaaggctatcccttcattgtataacacctgctttcaggtctg *F tggtgtgtgtctttgaggttagaaccggcgaaagctttccagtagggtgttgaaaaatgagaggggtgcggggtaataca *F aattgacaataattgacattgtttataaaactatagttgttaatatcgggccaccaacaactatgctagtagcctctgaa *F gcacaacaaacacagcagccaaagtgccaatcgagggaaaattacttaacgattgcataacttggcattacagcagagca *F atgaacttggtcggcactcgagggttaagcagcagtattagtgtatttgccaagatccggtcatgtgtgatctaaatgcc *F caagcactaagtttctatttcacacacaaatagttcttacttgtagtacgatgtgaaggaatttcgggtatttatggaac *F attttccatagcattcctatacttataaattgaattggtaaacatgataaaccatgaaaagattatatgaatcaattctc *F aatttttgttgtctataagctattagtagtagattagtagtggtaatgggggttaggtaaaatattatgaattttatgca *F taatatatctcttatcacggccagaaactttatctcaagcaatgtacacacggcgtggaataccgcttatataatcaacg *F aacgctcatctacctttctagttcctcgccaactccaagcagtacttgcgaccattgagcagcgccatcatcagccagag *F ccagactttggccgctcagaacacaacccccgttgcattgctgccacagatcaggtcattccagacctcgccagtcacgc *F gtgacattgactcggccgccaaattcattggtgctggtgccgcaacagtcggtgtcgctggatccggtatgtattggttt *F cgttgggcacctacatcattcagtcagaggggatccccagacgccttgggcttcttttcctccagtcagttgcaaactag *F catctggggcccacacaacgagtatctgattactccacaaaccattgccccgggaaggtctgagaatcggccgagccagc *F tgtttgttgcggcttcatttcccagcaggaaacctgtgtgattgcagggcgaaagtaccagaaatcctgctaccaggtgt *F tgccgttgcccccggtgaccgccgcctggttggcattgaaacctttcgtggccagcgtttttagtgcgatgtgcttgctg *F cctctaaggcagaactcaattcagactaatctgtgactgactacctggtgaaagaagctaaaggcgcgtcagggtgaccc *F atccattccccattgtccgaattccatttctcacttgcgagcttcggcgagaaccggagatgccaactgacttggacagg *F gtactaatgctttgatcttctttctcctcccaacttcgcgaccgaaacacaacgaaccaatacaacaggtgctggtatcg *F gaacagtattcggttccctcatcatcggctacgccaggaacccatcgctgaaacagcagctgttctcctacgccattctg *F ggcttcgccctgtccgaggccatgggtctgttctgtttgatgatggccttcctgctgctgttcgccttctaagcgcgcct *F gctgcacatcgattgctcgtccttgtttcctgcgatcgggcgcgtgcgccagtcgcatgcgaacgagaatagcaaccaaa *F caacacttcaaacaaacaaacaacaacaacaaccagaacaagcaacacctacgttcgttttcgattattaatgataaaat *F gattgattgattgatagcaaacaagaacatcctgtacaactatctaattataaataccaacaacaaccacataatacatg *F taattttgtttttaaaaaacatgcgagcgaggagaagaccacacctactttaagttgaacaaccgaaatatcaggaggaa *F ggaggattggattcaatcggattggactagccaacgctcacgcccatacccagacgacagacattaaaattagattttac *F ttaaagcaagcttccggctcaccgaaaacacaacccaatcgatggcccgaacgaactccagccatcctcgtgcactcggc *F ccattcggcggaggatctccatgcgcgcccaccaaggctctctctctccccgaagttcttggacatgcggcaggcatctc *F gtggagatcacctctgcgactaacatcttggatgggcgagtgtgtgggcgtggctgggcggagatgcggtggatcgttga *F gctcagcaacttttcgattttattgtggcgcgtactgaaagtctacgttttttcaagtattttgtattcgaaaaaatcag *F tttctttatgtaaaagctgtctttgttcattgccacttaatatttatgaagaaacttgtactaagagtagttgcaaacga *F aatgagccgaagagcgcagctgaaatctgaaataaaggagaaatgaatgtgtaatggaatcttggccaagtttgttttta *F tttaatgatttacttccttgataatttacacagaatcgtctaattttcggtagttcaaatttcggcgcccgaatgcagct *F ctgttgtgctaggaccgtagtcaccctgtaattggcagaacctatcgacacaagtagccctgtaataattgttagtgatg *F tgaaataatagatctgcttatcgataacaattgcgccgcacgtgcacaattgtttattttgatttttcacatttcttgtg *F atggatttcgccaagaagatactgggaaaatacggctggaaagagggcgacggattgggcaagaacaacaccggaatcgc *F agttcccttgaaggccgccttgaagttcgataacgcgggactgggagtggatcgcgcccaggaattcaatgaccattggt *F gggagcgctgctttaacgaggccgccagcaatgtggacgtccagattcagcaggacggacaggtgtccacctcccgcaga *F aagggcgaggaagcggtggagatatccactagaggattctccgcgcgcaagctgaagaaagcgaaggagcagcacgcaag *F cgatggaaaggccacctacgacaacttcctgcagacttcgctgctcacgcagagcggtggagaggttgagacctccgaac *F gcatcagggtggaggacattgaggtcaccaaggtggcggtactcacagatgcggaactcttcaaggcctgcggaggaagg *F actgcgcacaaaggagcacggcatggcctaaagctgagcggaaagatcgcccgcctggagcagcaggagcgcgagatgct *F ggagaagcttcagcgcaagctgaagactacgcctgaaaccgagcatcgagcagtggagtcagttgatgacagtgtggagc *F agtgtgatacgaaggcaaagaaaaagaaaaaatccatagcggaggagttttccgaggcatgtgaggtttcccaactggaa *F gagcccataaaatccaagaaaaagaagaaagacaag *F Browser: Mozilla/4.08 (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131171 *a Busseau *b I. *t 2000.10.18 *T personal communication to FlyBase *u *F From Isabelle.Busseau@igh.cnrs.fr Wed Oct 18 09:08:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 18 Oct 2000 09:08:26 \+0100 *F From: Isabelle.Busseau@igh.cnrs.fr *F Date: Wed, 18 Oct 2000 10:16:32 \+0200 *F Subject: Communication *F To: flybase-updates@morgan.harvard.edu *F X-Authenticated: *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='10415530--1619001344--1114114302=:9000' *F Content-Length: 44079 *F Dear Flybase, *F We have identified two new non-LTR retrotransposon in the genome of *F Drosophila melanogaster, Waldo-A and Waldo-B. They are described in an article *F which is in press in Mol. Biol. Evol.: *F Busseau I, Berezikov E and Bucheton A (2001) Identification of Waldo-A and *F Waldo-B, two closely related non-LTR retrotransposons in Drosophila. *F Mol. Biol. Evol., in press. *F We think that it would be useful to the Drosophila community that these *F elements appear in the databases. We are not sure about the way to do this. *F You will find attached two rtf files indicating the coordinates of the Waldo-A *F and Waldo-B elements in GenBank sequences accession numbers AC005734 and *F AC005847, that were produced by the BDGP. Please let me know if these files *F are useful to you, if we should provide additional information, if we should *F contact directly GenBank and/or BDGP. *F Sincerely yours, I Busseau. *F \------------------------------------------------------------------ *F Isabelle Busseau *F IGH CNRS *F 141 rue de la Cardonille *F 34396 Montpellier cedex 5 *F Tel 04 99 61 99 48 *F Fax 04 99 61 99 01 *F E-mail Busseau@igh.cnrs.fr *F \------------------------------------------------------------------ *F We have identified a new non-LTR retrotransposon in the genome of Drosophila *F melanogaster. We have named it Waldo-A. *F Reference: *F Busseau I, Berezikov E and Bucheton A (2001) Identification of Waldo-A and *F Waldo-B, two closely related non-LTR retrotransposons in Drosophila. *F Mol. Biol. Evol., in press. *F The Waldo-A element was identified by analyzing the sequence in GenBank *F accession number AC005734. Coordinates: 107957-102787. *F 107963-107958: putative Target Site Duplication. *F 107575-106079: ORF1. Similar to retrovirus gag proteins with cysteine-rich *F motifs; possible nucleic acid binding protein. Translation: *F MDSAVGGSPPDRNDPFRRSPRLSRSPTRGVGNPTHGDGISVPSVARGEHA *F GPQPPLTSDCSDAVEVENLAPTASTTATSQKTMDHIAVPSQKTGGKSPAG *F SPHRSSDLTAALQNEDLRGILTRMNGKISTLLSAFETQRHVTRETKGIAS *F ELSALNNRALELYKGTTSEHRLKSSATQTEVQKPTKKAPQNAQVPKVQVP *F KIQAPKPSNPTLGGNGEEKATSRPNESKPGSYASVAKNASTGVEWTKVKP *F TRLRKKPEALIVKKTGEASYAEMLRKLRSDPSLSELGSHVRKIRRTQKGE *F LLLEVEGKASESVPKFKSDLEAALNDLASVRTGAQRIALSCSGLDEATTA *F EELHSCLVAQFQGLQINPEDIRGLRRMRDGTQIASVLLNANVAIPVLKQG *F TITVGWSRCRITQDVRPTRCYRCLGYGHRSATCKNTDRADCCLRCGERGH *F KAKGCVAAPKCLICSSEVDRNHSTGSFACPTYRATLKEAKSHLNAHSY *F 106095-103132: ORF2. Probable reverse transcriptase. Translation: *F MHTHISVVQLNVNHCAAAQSLLAQTAAERNVDIMLLSEPYVSGSGQSSMI *F LDETGKAAIKCCSSLHVEELAALPMRGIAYAKLKHVHLYSCYAPPSDTPD *F QFEEFLEALVDHARGRSPKVIAGDFNAWAVEWGSRTSNTRGRAVIDAMGM *F LDLILLNDGRKPTFNNDRGTSFIDVTFVSRGLVDNNNWMVHDVMTLSDHA *F LISFSLSPEDMPRRRQSRAVGKAWDTRKIDEAMLAYQINSLEIPSGDAES *F MAAGLMNMLGRICDAIMPRKNKAQRKPPVYWWSASLSQLRSDCLRARRMA *F QRARGSTHHAELLEAFRRKRLEFKHGIAAAKARSFKELQDGVDSDTWGLA *F YKLVTKKLRRRAATPSDPGVLANIVGELFPKQTTLWRPTEAAPAPDFPCV *F TELEVAEAAKRIKPNKAPGLDGIPGAVIKAVALGRPEIFRATFQQCLLDG *F IFPTRWKSQKLVLLPKGKGPAHAANSYRPLCLLDIVGKLFERILYTRIEA *F ITESINGLGSHQYGFRKGKSTLDALSAVCNIAKTAISGDRWLGGRKEYCA *F IVTLDVRNAFNTARWPVILAAMYRMGIPEYLRIVVGSYFRDRVLWYDTED *F GPKRYRVSAGVPQGSVLGPILWNIMYDGILGINRPVGVELHCFADDVAIT *F AVSKTIAGLEDKCNSTIGAAIRWLEKAGLAIAAHKTEAVLLSSRKKVENM *F LVSVKGTQVTSQESLKYLGVMIDRRLSFKDHASHASKKAAITASSLARLM *F PNVGGPRHPARKLLVSVAKASLLYAAPVWSNATGRVSYLKGARSVLRSMS *F LRLIRGFRTISEDAALALAGLPPIDLEIKALSLMRSGASRQEAHEWLLGE *F WQSRWQTSRRGRWTYQLIPEMTVWAECQHKCLDYHLTQFLTDHGCFRAYL *F LRFRHVESAQCLFCVDGEETAEHVLMHCSRFTAEREQLKTLSGSPFSPSG *F LFAAMMANRGAWERGHSIIINMMKRVRSDEMANRVDV *F 102786-102781: putative Target Site Duplication. *F Full sequence of the Waldo-A element (5171 bp): *F GCTGCCAACTTGGCGCAGCGGGCGATCTGGAGAGGGAGGCGTTGCCAGAT *F CAACCGTGTGATCAGTGTTGTGTTGCGACAGTAGAGCGCGAAATTCAAAA *F TTAAACTTAAAATTAAACCGTTGAATTTTAATTTAATTTTAATTTTGATT *F CTGGTTACTGGGAGCGCACAAGTCTTTAAGAAAGGGGAAAAGGCACAAAG *F GTCGAGGACAGACCAAATATCATCCTCTCACCGTGCTGGTGCCTAAGCAG *F AGGCTCTGGTGTCCGAGTCGAGGCGGTACAAACCTCCATCCGTAAAAGCC *F AGGGGGAAATCCGTGGCCTACGTGGGAACCCCTTTCTAAGGCTGGTGCTT *F CGCCTAGGTAACCTTAGGCCATCTTTCAGTTGATGGACAGTGCAGTGGGG *F GGCAGTCCCCCTGATCGGAACGACCCGTTCAGGAGGAGCCCCCGACTTTC *F GAGATCCCCCACCAGAGGAGTCGGTAACCCGACCCATGGTGACGGAATCT *F CGGTCCCGTCGGTGGCAAGGGGTGAGCACGCAGGCCCCCAGCCGCCGTTG *F ACTAGCGACTGCAGCGATGCTGTAGAGGTGGAGAACTTGGCTCCTACAGC *F ATCTACGACCGCGACAAGTCAGAAGACGATGGACCACATCGCCGTGCCAT *F CGCAAAAGACGGGAGGGAAGTCCCCAGCTGGTTCCCCTCATCGGTCTTCG *F GACCTGACCGCGGCTCTCCAGAATGAGGACCTGAGGGGCATACTTACCAG *F GATGAATGGCAAGATCAGCACCTTGCTATCCGCATTCGAGACCCAACGGC *F ACGTGACACGAGAGACAAAGGGCATAGCTTCCGAACTCTCAGCCCTTAAC *F AACAGGGCGTTAGAGCTGTACAAAGGTACTACTAGCGAGCACCGCTTGAA *F GAGCAGTGCTACCCAGACGGAGGTACAAAAGCCTACCAAAAAGGCTCCTC *F AAAATGCACAGGTACCCAAGGTGCAAGTGCCAAAAATCCAGGCGCCCAAG *F CCGAGCAACCCTACGTTGGGTGGCAACGGTGAAGAAAAGGCAACATCCAG *F ACCAAACGAGTCCAAACCAGGCAGCTACGCATCTGTCGCCAAGAATGCTA *F GCACGGGTGTAGAGTGGACCAAGGTGAAGCCTACGCGCCTGCGTAAAAAA *F CCGGAGGCACTCATCGTAAAAAAGACAGGAGAGGCTTCGTACGCAGAGAT *F GCTTCGGAAGCTAAGATCGGACCCGAGCCTTAGCGAACTGGGCAGCCACG *F TGCGAAAAATCCGGAGAACGCAGAAAGGTGAGCTGTTGCTCGAGGTAGAG *F GGGAAAGCTTCGGAAAGCGTCCCCAAGTTTAAGAGCGACCTGGAAGCGGC *F GCTCAATGACTTGGCCTCTGTGCGCACAGGAGCGCAAAGAATAGCTCTAT *F CTTGCAGCGGATTGGACGAGGCTACGACAGCAGAGGAGCTCCACAGCTGC *F TTGGTCGCCCAATTCCAGGGCCTGCAGATAAATCCTGAAGATATCAGGGG *F CCTTCGCAGAATGCGGGATGGCACGCAAATAGCCTCAGTGCTGCTGAACG *F CGAACGTTGCGATACCAGTCCTTAAACAGGGCACCATAACCGTTGGATGG *F TCAAGATGTCGTATCACCCAGGACGTTCGACCCACGAGATGCTACAGGTG *F TCTCGGCTATGGGCATCGATCAGCAACCTGCAAGAACACTGACAGGGCAG *F ACTGCTGTCTTAGATGCGGTGAGCGTGGGCACAAGGCAAAGGGGTGCGTT *F GCAGCACCAAAATGCCTGATCTGCAGCAGCGAGGTGGACAGAAACCACTC *F GACGGGTAGCTTTGCGTGCCCGACCTACAGAGCGACCCTAAAAGAAGCCA *F AGAGCCACCTTAATGCACACTCATATTAGCGTAGTACAGCTCAATGTCAA *F TCATTGCGCAGCAGCTCAGAGCCTCCTGGCCCAGACTGCGGCTGAGCGCA *F ATGTAGACATCATGCTCCTAAGCGAACCCTACGTCTCTGGTAGCGGACAA *F TCGTCCATGATCCTTGACGAGACAGGTAAAGCAGCTATCAAATGCTGCAG *F CTCTCTCCACGTCGAGGAACTGGCTGCTTTACCTATGCGGGGTATCGCTT *F ATGCGAAGTTAAAACACGTGCACTTGTACAGCTGCTACGCTCCGCCGAGC *F GACACCCCCGATCAGTTCGAGGAGTTTCTGGAGGCGCTCGTGGACCATGC *F GAGAGGGCGAAGCCCGAAGGTCATTGCCGGCGACTTTAATGCCTGGGCAG *F TGGAATGGGGCAGCAGGACATCCAACACCAGAGGCCGAGCTGTGATTGAC *F GCCATGGGAATGCTGGACCTTATACTGCTGAACGACGGACGGAAGCCGAC *F GTTTAACAACGATAGGGGTACGTCCTTTATTGACGTTACCTTTGTCAGCA *F GAGGGCTAGTAGACAACAATAACTGGATGGTCCATGACGTCATGACGCTG *F AGCGACCACGCCCTGATCTCCTTCAGTCTCTCCCCGGAGGACATGCCCAG *F GAGACGGCAGAGTAGAGCAGTCGGGAAAGCATGGGACACCAGGAAGATCG *F ATGAGGCCATGCTGGCCTATCAGATCAATTCCCTGGAAATCCCAAGTGGG *F GACGCAGAGAGTATGGCGGCAGGCCTCATGAATATGCTGGGAAGAATCTG *F CGACGCAATCATGCCAAGGAAAAATAAGGCACAGCGCAAACCACCCGTTT *F ACTGGTGGAGCGCCTCCCTAAGCCAACTACGGTCTGATTGCCTCAGGGCT *F AGGAGAATGGCGCAACGAGCCAGAGGCAGTACCCACCACGCGGAACTCTT *F GGAGGCTTTCAGAAGGAAACGTCTAGAGTTCAAGCACGGCATCGCGGCTG *F CCAAAGCGCGGTCGTTTAAGGAGCTGCAGGATGGCGTAGACAGCGATACC *F TGGGGCCTCGCCTACAAGCTTGTTACCAAAAAGCTAAGGAGGAGAGCGGC *F AACCCCATCCGACCCGGGGGTCCTGGCTAACATAGTAGGGGAGCTATTCC *F CAAAGCAGACCACACTATGGAGGCCAACAGAGGCAGCCCCTGCCCCAGAT *F TTTCCGTGCGTCACAGAACTTGAAGTCGCCGAGGCAGCCAAGCGCATCAA *F ACCCAACAAAGCCCCTGGACTAGATGGTATTCCTGGAGCTGTTATAAAAG *F CAGTGGCGCTGGGTAGACCTGAAATCTTCAGGGCCACCTTCCAGCAATGC *F CTTCTGGACGGAATCTTCCCAACAAGGTGGAAAAGCCAGAAGCTAGTCCT *F GTTGCCGAAAGGCAAGGGACCAGCACATGCTGCAAACAGCTACCGCCCTC *F TATGCCTACTGGATATAGTAGGAAAACTGTTCGAACGTATCCTGTATACC *F AGAATAGAGGCAATCACCGAGAGCATCAACGGCCTGGGAAGTCATCAATA *F TGGCTTCCGGAAAGGTAAGAGCACTCTGGACGCTCTTTCGGCCGTTTGTA *F ACATCGCCAAGACCGCTATTTCTGGTGATAGATGGTTAGGGGGCAGGAAG *F GAATACTGCGCAATTGTGACTCTGGACGTAAGGAACGCTTTCAACACCGC *F CAGATGGCCCGTAATCCTCGCGGCCATGTACCGTATGGGGATCCCGGAGT *F ACCTAAGGATAGTCGTTGGCAGCTACTTTAGGGACCGGGTCCTATGGTAC *F GATACGGAAGATGGCCCAAAAAGATACCGAGTTTCGGCAGGTGTTCCCCA *F AGGATCGGTACTTGGACCAATCCTATGGAACATTATGTACGATGGGATCT *F TGGGCATCAACAGGCCCGTAGGAGTAGAGCTGCATTGTTTTGCTGACGAT *F GTGGCAATCACAGCTGTCTCGAAAACAATCGCAGGGTTGGAAGACAAATG *F CAACTCTACGATCGGTGCTGCCATCCGCTGGCTCGAGAAAGCCGGGCTAG *F CAATAGCGGCTCACAAGACCGAAGCAGTCCTACTAAGCAGCAGGAAAAAG *F GTGGAGAACATGCTGGTCTCCGTCAAGGGTACACAGGTGACCTCTCAAGA *F GTCCCTAAAGTACCTGGGGGTAATGATAGATCGCAGACTATCGTTCAAGG *F ACCACGCGAGCCACGCCAGCAAGAAGGCAGCAATCACAGCCTCTTCGTTG *F GCGAGGCTTATGCCCAACGTCGGAGGCCCAAGACACCCGGCCAGGAAACT *F GCTGGTGTCAGTAGCAAAGGCTTCGCTACTATACGCTGCACCAGTCTGGA *F GCAATGCCACTGGCAGGGTCTCATACCTGAAAGGAGCTCGTTCGGTGCTA *F CGGTCAATGTCTCTGAGGCTCATTAGAGGTTTCAGGACCATATCCGAAGA *F CGCGGCGCTAGCGCTGGCAGGCCTGCCGCCGATTGATCTGGAGATCAAGG *F CTCTCAGCCTAATGCGGAGTGGCGCTTCCAGGCAAGAGGCACACGAGTGG *F CTATTAGGTGAATGGCAGAGTAGATGGCAAACGTCGCGACGGGGGAGGTG *F GACTTATCAGCTCATCCCAGAGATGACGGTTTGGGCAGAGTGCCAACACA *F AATGCTTGGACTACCACCTAACCCAGTTCCTCACGGACCATGGCTGCTTC *F CGGGCCTATCTACTCCGGTTCCGTCACGTAGAGTCAGCCCAATGCTTGTT *F CTGCGTCGACGGTGAAGAAACAGCAGAACATGTGCTAATGCACTGCTCCA *F GGTTCACGGCGGAGAGAGAGCAGCTAAAGACGCTGTCAGGTTCCCCGTTC *F AGCCCTAGTGGCTTGTTCGCGGCTATGATGGCGAACAGGGGGGCTTGGGA *F GCGGGGACACAGCATTATCATTAATATGATGAAGCGTGTCCGATCAGACG *F AGATGGCCAACAGAGTGGATGTCTAAGCCCAAACTGGTGTCCTGGGTGAC *F GGCGGGCGAAGAATTCATCCTCAGCGTCCCCGGCTCGTCGTAAAAGGCGA *F CTAAAGGGTGGAAGGAGGAGCCCCCATGGACTACACTGAAGGAAGGGAGT *F GCGACCTGGCCTCACATCCTGCTCACCGAAGTCATACCTTGACTGGCAGT *F CCCGGTGAGCGAGCAAGGACTGTAGAGCACGCGGAGGTTTTTGTTTTAGT *F ACGTAGGCATAATTCCAATAGGGCTTATGAATCGTGCATGCCACCTACGG *F ACGGTAGGTGGTATCTTTAGAAGATTTTAATTTTCCTACCGTAAGTCAAA *F TAATAAAAAAAAAAAAAAAAA *F We have identified a new non-LTR retrotransposon in the genome of Drosophila *F melanogaster. We have named it Waldo-B. *F Reference: *F Busseau I, Berezikov E and Bucheton A (2001) Identification of Waldo-A and *F Waldo-B, two closely related non-LTR retrotransposons in Drosophila. *F Mol. Biol. Evol., in press. *F The Waldo-B element was identified by analyzing the sequence in GenBank *F accession number AC005847. Coordinates: 20184-15005. *F 20195-20185: putative Target Site Duplication. *F 19750-18284: ORF1. Similar to retrovirus gag proteins with cysteine-rich *F motifs; possible nucleic acid binding protein. Translation: *F MDRTGGGSAPDPNDPFRRSGRLSRSPIRGVGTQIQGGGGEQAGPPPPKAS *F DCSAAVEVETLATTVSTTATSLKTMDFISVPAQRAGAKSPSGSPHRSPEL *F TTTLQNEDLQGILDMMKAKITAILSSFETRRHVTSEDRGVLVDLSALNKR *F AIELQEGINKKPPPRSTATQTEAEKTKRSQVAPPRQALPNVQVRKTDNHR *F SAAKSTGKALPTTADSKPESYASVAKDANKDEEWAKVKPKRLRKKPEALI *F LKKTGEVTYSDMLRKMKAEPSLTEFGKHVRKIRRTQQGELLLELEGKASE *F VIPSFKNELEATLKEIASVRTGAHRTALICSGLDETTTAQDLHNSLVSQF *F QGIRLEPEDVRGLRRRRDGTQIASVLMCANDAIAVINRGVVTVGWSRCRI *F AQDVRPIRCFRCLEFGHRAPYCKSVDRSDCCLRCGEHGHKAKGCVAPPRC *F LICSSDVDKNHATGGFACPTYKANTKGANSRQNDARRN *F 18303-15334: ORF2. Probable reverse transcriptase. Translation: *F MMPEEINIIQLNVNHCAAAQNLLTQTAKERHADVVLLSEPYLPGVGNSGV *F LLDETGKAAIKCTSRLLVEEWDTVPMRGIAYAKIRGIHFYSCYAPPSDSP *F EQFEDMLEKLVNHASGRRPTVIGGDLNAWATEWGSRISNTRGRAVIDAMN *F LLDLVLLNDGFKPTFNNDRGTSFIDVTFVSRVLVAGSNWMVHEDITLSDH *F NLITFGARTMRPSPKQRRCALGPVWDIRKLDEDMLAYQIEGMESIIGHAE *F TMVTALMDRLRAMCDAVMPRKRNTKRKPPVYWWSDSLHQLRTECIKARRQ *F AQRSRGQPHHSQCIEVYKAKRTELKNGISAAKANAFKDLIDSVDDDPWGL *F AYKVVRKKLNSAGAGSPQDPAALANIVSELFPSQHTLWQPAVDPPASDFP *F CITSCEVVEAAKRIRPNKAPGIDGIPGVIVKAAATARPEVFRDTFQQCLL *F DGVFPKRWKKMKLVLLLKGKGPANVPRSYRPLCLLDIVGKLFERILYARI *F ELITESPTGLQGQQYGFRKGKSTLDALKSVTDAARKALDGNRWLGGSKKY *F CAIITLDVKNAFNTARWPIILGAMRNLGVPDYIRGVIGNYFRDRVLWYVT *F EDGPRSHQVSAGVPQGSVLGPILWNIMYDGILSISKPRGVELHCFADDVA *F ITAVAKTIPELQDISNVAITAAIEWLEKVGLKIAAHKTEVVLLSSRKSVE *F CMRVEVKGVEIASAETLKYLGVLIDRRLSFKAHARYASKKAAMTAAALAR *F IMPNVGGPRLPARRLLVAVSKATLLYAAPIWSCVSTKKTYLDSARAVSRT *F MALRLIRGFRTISDDAAHALSGITPIDLDIKGKYLASEGYTQLEIKEWIR *F GVWQTRWQESQRGRWTYKLIPQLTEWADCEHKTVDYHMTQFLTDHGCFRG *F YLCRFRHVDTAQCLYCTDAVETAEHILLHCSRFAEERAQLVALAGSPLSP *F RGLVAAMMADKIVWDGAHVIIVTMMKRVRKDEMANRNYR *F 15004-14994: putative Target Site Duplication. *F Full sequence of the Waldo-B element (5180 bp): *F CCGCGTATTTCGCACGTCTTTTTGCTGCGCTGCGCGAATTTCGTCATATA *F CGAGTCCGGTCAGCCGTAAACTGCAACATAAACCTGCAAATCCACTCTGG *F GGTTTGCGCCGCGTTTCTGAGCGTCACGGTGTCGTTCCGGAATTCACGAG *F TGATCCAGTAAACTGGTAATAAATCAGATCAGCTGCATCAGACAATACAG *F CTGATAGCATTGCCAACCTTTCGCAGTCGACGAGCTGGTTAGACTGGCGT *F TGCCAGATCAGCGGTGCGATCGTCATTCGGCCGCGCGCTCGTTGGGCGCG *F AAATTCGAATTCAAATTCAAATTTGAACTAATTAAGTTGAACAAAAATTT *F GAAATATAACCTAAGAACTGAAGCTAGTTCTATTTCCCACGGAGCGCTGG *F GGACCAACAAGCCAAAGTCCCCCTTTTATATCTGATGGACAGAACAGGGG *F GGGGCAGTGCCCCCGACCCCAACGACCCGTTTAGGAGGAGTGGTAGGCTA *F TCGAGATCCCCTATAAGAGGAGTCGGAACCCAAATCCAGGGAGGGGGAGG *F TGAGCAAGCAGGCCCCCCGCCGCCGAAAGCTAGCGACTGCAGTGCAGCTG *F TGGAGGTGGAGACTCTGGCCACTACAGTCTCCACGACAGCCACAAGCCTG *F AAGACAATGGATTTCATCAGCGTGCCGGCCCAAAGGGCGGGAGCGAAGTC *F CCCATCAGGATCGCCGCATCGCTCGCCAGAACTGACCACGACGCTCCAGA *F ATGAGGACCTGCAGGGCATCCTGGACATGATGAAAGCCAAGATCACCGCC *F ATTCTATCCTCGTTCGAGACTAGGCGTCACGTAACCAGCGAGGATAGGGG *F CGTGCTAGTGGACCTATCGGCGCTCAATAAGAGAGCGATTGAGTTACAGG *F AGGGTATCAACAAGAAGCCCCCACCAAGGAGTACTGCCACACAGACTGAG *F GCAGAAAAGACAAAGCGTAGCCAGGTGGCGCCACCCAGACAGGCATTACC *F GAATGTGCAGGTCCGCAAAACGGACAACCATCGATCTGCCGCGAAATCCA *F CCGGGAAGGCGTTACCCACGACGGCTGACTCCAAACCGGAAAGTTATGCG *F TCTGTCGCCAAGGACGCTAACAAGGACGAGGAATGGGCTAAGGTGAAGCC *F CAAGCGCCTGCGCAAAAAACCTGAGGCGCTAATCCTGAAAAAAACGGGTG *F AGGTTACGTACTCGGATATGCTCCGGAAGATGAAAGCAGAACCGAGTCTG *F ACCGAATTCGGCAAGCACGTGCGTAAAATAAGGAGGACGCAACAGGGAGA *F ACTACTTCTTGAATTAGAAGGTAAAGCCTCGGAGGTCATCCCCAGCTTTA *F AAAATGAGCTAGAAGCGACGCTCAAAGAGATTGCTTCGGTTCGCACGGGC *F GCGCATAGGACTGCGCTAATCTGCAGCGGACTAGACGAGACAACGACGGC *F TCAGGACCTTCACAATTCCCTGGTCTCCCAATTTCAGGGCATCCGCCTGG *F AACCAGAGGATGTAAGAGGCCTTCGCAGGAGGCGTGACGGGACCCAGATA *F GCCTCTGTGCTAATGTGCGCGAACGATGCCATTGCGGTCATCAACCGGGG *F CGTTGTAACTGTGGGATGGTCGCGTTGCCGCATAGCCCAAGACGTCCGCC *F CAATAAGATGCTTCAGATGCCTCGAATTCGGCCACCGAGCTCCCTACTGC *F AAGTCAGTCGATCGCTCTGACTGCTGCCTACGGTGCGGCGAGCATGGGCA *F TAAGGCAAAGGGCTGCGTAGCCCCACCAAGATGCCTGATCTGCAGCAGTG *F ACGTGGACAAGAACCACGCGACGGGTGGTTTTGCATGCCCCACCTACAAA *F GCCAACACCAAAGGAGCTAATAGCCGTCAAAATGATGCCAGAAGAAATTA *F ACATCATCCAGCTCAACGTTAACCATTGCGCAGCAGCACAGAACCTCCTG *F ACTCAAACAGCGAAGGAGCGCCATGCGGACGTAGTGCTTTTGAGTGAACC *F ATACCTACCTGGTGTGGGTAACTCAGGAGTGCTACTCGATGAGACAGGCA *F AGGCGGCCATTAAATGTACATCCAGGTTATTGGTAGAGGAGTGGGATACC *F GTACCAATGCGCGGCATAGCATACGCCAAAATTAGAGGAATCCACTTCTA *F CAGCTGCTATGCTCCACCTAGCGACAGTCCCGAGCAATTCGAGGACATGC *F TCGAAAAGCTGGTTAACCATGCAAGTGGGCGCAGACCAACAGTCATCGGA *F GGTGACCTCAATGCCTGGGCTACAGAATGGGGCAGTCGAATCTCTAACAC *F AAGAGGACGAGCAGTGATCGATGCCATGAACCTGCTAGATCTCGTATTGC *F TAAACGACGGGTTCAAACCGACGTTCAATAATGACAGAGGTACATCTTTC *F ATTGATGTCACTTTTGTTAGCAGAGTTCTAGTGGCTGGCTCGAACTGGAT *F GGTCCATGAGGACATAACGCTGAGCGACCACAACCTAATCACGTTCGGTG *F CCCGAACAATGAGGCCGTCACCCAAACAGCGAAGATGTGCGCTAGGTCCG *F GTATGGGACATTAGGAAACTGGATGAAGACATGCTAGCATACCAGATCGA *F GGGCATGGAGTCCATAATTGGACACGCGGAGACCATGGTGACAGCGCTCA *F TGGATAGGCTTAGAGCAATGTGTGATGCGGTGATGCCGAGAAAAAGGAAC *F ACGAAACGAAAGCCCCCTGTCTACTGGTGGAGTGACTCATTGCACCAGCT *F TCGGACGGAATGCATCAAGGCGAGGAGGCAAGCGCAGCGATCTAGAGGGC *F AACCGCACCACTCCCAGTGCATTGAGGTATATAAAGCGAAGCGAACTGAG *F CTTAAGAACGGCATATCAGCAGCAAAGGCGAATGCTTTTAAGGACCTGAT *F TGATAGCGTAGACGACGACCCCTGGGGTCTCGCCTACAAAGTCGTAAGGA *F AAAAACTCAATTCCGCTGGTGCAGGATCTCCTCAGGACCCAGCCGCCCTG *F GCCAACATCGTGTCGGAACTATTTCCAAGCCAACACACGTTATGGCAACC *F CGCTGTTGACCCCCCCGCCTCTGACTTCCCGTGCATCACGTCATGCGAAG *F TCGTCGAAGCAGCAAAGAGGATCAGACCGAACAAAGCTCCCGGCATTGAT *F GGTATCCCGGGCGTGATCGTCAAAGCAGCCGCCACCGCAAGACCGGAGGT *F CTTCAGGGATACATTCCAACAGTGTCTGCTGGACGGAGTCTTCCCCAAGC *F GCTGGAAAAAGATGAAGCTGGTCCTTCTGCTGAAAGGCAAGGGGCCCGCA *F AATGTTCCACGCAGTTACCGACCGTTGTGTCTGTTGGACATTGTTGGCAA *F GCTCTTTGAGCGCATACTGTACGCGCGCATTGAGCTAATCACTGAAAGTC *F CTACAGGCCTTCAGGGCCAACAGTATGGCTTCCGAAAGGGTAAAAGTACG *F CTCGACGCTCTCAAATCCGTAACAGACGCTGCCAGGAAAGCACTCGACGG *F TAACCGATGGTTAGGCGGCAGTAAGAAGTACTGTGCCATCATCACGCTAG *F ACGTCAAGAACGCATTCAATACAGCGAGATGGCCCATTATCCTCGGGGCT *F ATGCGCAACTTGGGCGTTCCCGACTACATACGAGGCGTAATTGGCAACTA *F CTTCAGGGACCGTGTGTTATGGTACGTAACAGAAGATGGTCCAAGAAGCC *F ACCAAGTCTCTGCAGGCGTTCCCCAAGGGTCGGTACTAGGACCGATCTTG *F TGGAACATTATGTATGATGGAATACTGAGCATTAGCAAGCCCAGAGGTGT *F GGAGCTGCACTGCTTCGCCGACGACGTTGCGATAACAGCGGTCGCCAAGA *F CAATACCGGAGCTCCAGGACATAAGTAACGTGGCAATCACGGCGGCCATA *F GAATGGCTCGAGAAAGTCGGACTTAAAATAGCTGCGCATAAGACCGAAGT *F AGTCCTGCTGAGCAGCAGAAAGTCCGTTGAGTGCATGCGTGTAGAAGTCA *F AAGGAGTTGAAATCGCCTCAGCAGAAACGTTGAAATACCTTGGTGTTCTA *F ATAGACCGAAGGCTCTCGTTCAAGGCTCACGCAAGGTATGCCAGCAAAAA *F AGCGGCAATGACAGCAGCAGCCTTGGCGAGAATCATGCCCAACGTGGGAG *F GACCCAGACTGCCGGCTAGGAGACTGTTAGTGGCGGTTTCAAAGGCAACG *F CTGCTTTATGCTGCGCCTATCTGGAGCTGCGTTTCCACAAAGAAAACCTA *F TCTAGATAGTGCCCGCGCAGTATCACGGACAATGGCTCTCAGGCTAATCA *F GAGGCTTTAGAACCATATCGGACGACGCAGCGCACGCTCTGTCAGGCATT *F ACACCCATTGACCTGGACATAAAGGGCAAATACCTTGCGAGTGAGGGATA *F CACTCAATTAGAGATCAAAGAGTGGATTCGAGGAGTATGGCAGACCAGGT *F GGCAAGAGTCACAACGGGGACGCTGGACTTACAAACTCATTCCGCAACTA *F ACGGAGTGGGCTGATTGCGAGCACAAAACGGTGGACTACCACATGACCCA *F GTTCCTCACGGACCATGGCTGTTTTCGAGGGTACCTATGTAGGTTCCGCC *F ACGTGGATACAGCCCAGTGCCTTTATTGCACAGACGCAGTGGAAACCGCA *F GAGCACATCCTACTGCACTGCTCCAGGTTCGCCGAGGAGAGGGCGCAACT *F CGTGGCGCTCGCTGGGTCACCTCTCAGCCCGAGAGGCTTGGTTGCTGCTA *F TGATGGCGGACAAAATCGTTTGGGATGGGGCTCACGTGATCATCGTCACC *F ATGATGAAGCGTGTCCGTAAGGACGAGATGGCCAATCGGAACTATAGATA *F AGGAGTACCCCCGTATGTTGGCGGGGCAAGAACTCTACGACTGGTACGCT *F CAGTTGGTCGTAAAAAGGCGCTGCTGTGCACCGCAAAAGAAGATGGTGTG *F CAACTTGGCACCACATCCTGCTCACCGATGAAATACCTTGACTGGCAGTC *F CCGGTGAGCTTGACAAGGACAGGAGAGAGAGCGGAGGTTTTTGTTTAGTA *F CGTAGGCATAAGCCCTCAGCTGAGGGTTATGAATCGTGCATGCCATCCAA *F GGACATTAGATGGTATCTTTAGAAGATTTCATTTTCCTGCCGTATATAAT *F AATAAAAAAAAAAAAAAAAAAAAAAAAAAA # *U FBrf0131172 *a Whitfield *b E. *t 2000.10.25 *T personal communication to FlyBase *u FlyBase error report for CG10580 on Wed Oct 25 02:25:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Oct 25 10:25:35 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Oct 2000 10:25:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Oct 2000 02:25:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10580 on Wed Oct 25 02:25:28 2000 *F Content-Length: 509 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10580 *F Gene annotation error *F Gene CG10580 has incorrect exon/intron structure. *F Comments: Please extend translation of fng (AE003592; AAF51658) to include the *F first Met *F encoded on the exon, ie extend the CDS feature by just 3 bases. *F The sequence is then a 100% match to Irvine and Wieschaus, Cell 79:595-606(1994) *F (L35770; AAA64525) *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131173 *a Whitfield *b E. *t 2000.10.24 *T personal communication to FlyBase *u FlyBase error report for CG7569 on Tue Oct 24 05:26:15 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 24 13:26:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 24 Oct 2000 13:26:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 24 Oct 2000 05:26:15 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG7569 on Tue Oct 24 05:26:15 2000 *F Content-Length: 940 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7569 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG7569. *F Comments: Celera genomic sequence has identified alternative splicing in DopR2. *F cDNAs from Feng et al, J. Neurosci. 16:3925-3933(1996) (U34383; AAC47161) and *F Han et al, Neuron 16:1127-1135(1996) (U61264; AAB08000) map to the genomic *F sequence and shows the cDNAs have alternate 3' exons. *F First release of Celera translated the longer isoform and the new release the *F sequence was changed to the other splice site to translate the shorter isoform. *F Please reinstate the first CDS feature: *F FT CDS join(complement(130944..132391), *F FT complement(130547..130628),complement(129334..129423)) *F so Celera shows translations of both isoforms. *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131174 *a Whitfield *b E. *t 2000.10.24 *T personal communication to FlyBase *u FlyBase error report for CG1658 on Tue Oct 24 04:34:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 24 12:34:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 24 Oct 2000 12:34:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 24 Oct 2000 04:34:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1658 on Tue Oct 24 04:34:39 2000 *F Content-Length: 631 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1658 *F Gene annotation error *F Gene CG1658 has incorrect exon/intron structure. *F Comments: Translation of Doa was perfect in the first Celera release *F (AE003767; AAF56832), *F now the translation in the new release has been truncated at the N terminus so *F it no longer encodes the first Met: *F please update *F FT CDS join(82151..82269,82390..82485,93209..93407, *F to *F FT CDS join(82133..82269,82390..82485,93209..93407, *F then all will be well *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131175 *a Whitfield *b E. *t 2000.10.24 *T personal communication to FlyBase *u FlyBase error report for CG17170 on Tue Oct 24 04:19:26 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 24 12:19:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 24 Oct 2000 12:19:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 24 Oct 2000 04:19:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17170 on Tue Oct 24 04:19:26 2000 *F Content-Length: 575 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17170 *F Gene annotation error *F Gene CG17170 has incorrect exon/intron structure. *F Comments: New translation of su(f) (AE002936; AAF45314) is now missing an *F exon, please *F revert translation to as it was in release 1 as this was a 100% match to a *F previous translation by Mitchelson et al, Genes Dev. 7:241-249(1993) (X62679; *F CAA44551). *F Translation now reads on the other strand \- just to help confusion! *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131176 *a Dolezal *b T. *t 2000.9.18 *T personal communication to FlyBase *u *F From gopher@firefly.bio.indiana.edu Mon Sep 18 07:52:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 18 Sep 2000 07:52:53 \+0100 *F Date: Mon, 18 Sep 2000 01:45:26 \-0500 (EST) *F From: fbmailer@bio.indiana.edu *F Reply-to: mladez@entu.cas.cz *F To: flybase-help@morgan.harvard.edu *F Subject: flanking sequence of l(2)k09935 *F Content-Length: 501 *F comments: Drosophila melanogaster P-element l(2)k09935 *F 5' flanking sequence is now available *F in Genbank with ACCESSION N. AF254926. *F Associated gene is 'mspo'. *F Tomas Dolezal *F Institute of Entomology *F Academy of Sciences of the Czech Republic *F Branisovska 31 *F Ceske Budejovice, Czech Republic *F phone: \+420-38-7775283 *F mailto: flybase-help@morgan.harvard.edu *F realname: Tomas Dolezal *F reply-to: mladez@entu.cas.cz *F Sent from computer yang.entu.cas.cz # *U FBrf0131177 *a Whitfield *b E. *t 2000.9.20 *T personal communication to FlyBase *u FlyBase error report for CG18372 on Wed Sep 20 08:29:28 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Sep 20 16:29:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 20 Sep 2000 16:29:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 20 Sep 2000 08:29:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18372 on Wed Sep 20 08:29:28 2000 *F Content-Length: 1073 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18372 *F Gene annotation error *F Genes CG18372 and AttB1 should be merged. *F Comments: Sequences differ by one residue, behold (Celera top line, published *F in Gene *F bottom line): *F MQKTSILILALFAIAEAVPTTGPIRVRRQVLGGSLASNPAGGADARLNLSKGIGNPNHNV *F MQKTSILILALFAIAEAVPTTGPIRVRRQVLGGSLASNPAGGADARLNLSKGIGNPNHNV *F VGQVFAAGNTQSGPVTTGGTLAYNNAGHGASLTKTHTPGVKDVFQQEAHANLFNNGRHNL *F VGQVFAAGNTQSGPVTTGGTLAYNNAGHGASLTKTHTPGVKDVFQQEAHANLFNNGRHNL *F DAKVFASQNKLANGFEFQRNGAGLDYSHINGHGGSLTHSNFPGIGQQLGLDGRANLWSSP *F DAKVFASQNKLANGFEFQRNGAGLDYSHINGHGASLTHSNFPGIGQQLGLDGRANLWSSP *F NRATTLDLTGSASKWTSGPFANQKPNFGAGLGLSHHFG *F NRATTLDLTGSASKWTSGPFANQKPNFGAGLGLSHHFG *F Celera: AE003813; AAF58214 *F Gene: EMBL; AF220546; AAF71234 *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131178 *a Teixeira *b L. *t 2000.10.14 *T personal communication to FlyBase *u FlyBase error report for CG11558 on Tue Oct 10 06:43:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 10 14:43:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 10 Oct 2000 14:43:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 10 Oct 2000 06:43:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: teixeira@embl-heidelberg.de *F Subject: FlyBase error report for CG11558 on Tue Oct 10 06:43:27 2000 *F Content-Length: 281 *F Error report from Luis Teixeira (teixeira@embl-heidelberg.de) *F Gene or accession: CG11558 *F Gene annotation error *F Genes CG11558 and CG1856 should be merged. *F Comments: They match same cDNAs *F Browser: Mozilla/4.61 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131179 *a Kunes *b S. *t 2000.10.23 *T personal communication to FlyBase *u FlyBase error report for CG8367 on Mon Oct 23 16:03:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 24 00:05:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 24 Oct 2000 00:05:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 23 Oct 2000 16:03:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kunes@fas.harvard.edu *F Subject: FlyBase error report for CG8367 on Mon Oct 23 16:03:41 2000 *F Content-Length: 429 *F Error report from SAM KUNES (kunes@fas.harvard.edu) *F Gene or accession: CG8367 *F Gene annotation error *F Genes CG8367 and combgap should be merged. *F Comments: We have found that the migraine gene (mig) is the same as the gene *F combgap. We will update Genbank with the correct identity of the cDNA *F sequence reported as the migraine sequence. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131180 *a Davis *b G. *t 2000.10.25 *T personal communication to FlyBase *u FlyBase error report for CG10052 on Wed Oct 25 10:33:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Oct 25 18:33:26 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Oct 2000 18:33:26 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Oct 2000 10:33:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gdavis@midway.uchicago.edu *F Subject: FlyBase error report for CG10052 on Wed Oct 25 10:33:20 2000 *F Content-Length: 364 *F Error report from Greg Davis (gdavis@midway.uchicago.edu) *F Gene or accession: CG10052 *F Missed gene *F Comments: In the FlyBase Report for Rx it is stated that the protein possesses *F a paired domain, which I don't believe it does (only a prd-like homeodomain). *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131181 *a Davis *b G. *t 2000.10.25 *T personal communication to FlyBase *u FlyBase error report for CG3935 on Wed Oct 25 10:28:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Oct 25 18:29:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Oct 2000 18:29:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Oct 2000 10:28:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gdavis@midway.uchicago.edu *F Subject: FlyBase error report for CG3935 on Wed Oct 25 10:28:56 2000 *F Content-Length: 363 *F Error report from Greg Davis (gdavis@midway.uchicago.edu) *F Gene or accession: CG3935 *F Missed gene *F Comments: In the FlyBase Report for al it is stated that the protein possesses *F a paired domain, which I don't believe it does (only a prd-like homeodomain). *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131183 *a Whitfield *b E. *t 2000.10.25 *T personal communication to FlyBase *u FlyBase error report for CG2033 on Wed Oct 25 02:01:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Oct 25 10:01:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Oct 2000 10:01:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Oct 2000 02:01:45 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2033 on Wed Oct 25 02:01:45 2000 *F Content-Length: 590 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2033 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG2033. *F Comments: I have noticed that the translation of CG2033 (AE003492; AAF48256) *F is 100% *F identical to that of RpS15A, Lavoie et al, submitted (FEB-1993) to the EMBL *F database (Z21673; CAA79771). *F Maybe the CG symbol should be replaced by a Ribosomal specific name? *F Just a suggestion, I don't know your policy for this *F thanks *F Browser: Mozilla/4.06 [en] (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131184 *a Millburn *b G. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG4207 on Thu Oct 26 06:56:00 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 14:56:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 14:56:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 06:56:00 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG4207 on Thu Oct 26 06:56:00 2000 *F Content-Length: 1811 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG4207 *F Gene annotation error *F Genes CG4207 and bonsai (FBgn0026261) should be merged. *F Comments: Hi, *F I think that CG4207 (FBgn0034749) and bonsai (FBgn0026261) are the same *F gene, because: *F 1. in FBrf0108515 == Galloni and Edgar, 1999, Development 126(11): *F 2365--2375 *F in Figure 4, bonsai is shown divergently transcribed from Cdk9 *F (FBgn0019949) in 58F, with the two transcription units 110bp apart: *F \-1 \+110 *F ________|__________|__________ *F | | *F <------ \--------> *F bonsai Cdk9 *F I looked at GeneSeen, and the predicted gene that is divergently *F transcribed upstream of Cdk9 is CG4207, and the gap between the start *F of the transcription units appears to be about 50-100bp: *F ________|_____|__________ *F | | *F <------ \--------> *F Cdk9 CG4207 *F 2. In FBrf0108515, bonsai is stated to 'encode a partial homolog of the *F prokaryotic ribosomal protein S15 and of the yeast mitochondrial *F ribosomal protein MRPS28. This suggests that bonsai encodes for a *F product involved in translation, potentially a mitochondrial ribosomal *F protein'. *F The evidence for CG4207 includes homology to: *F species == Aquifex aeolicus; gene == 'ribosomal protein S15'; EMBL:AE000679; *F gi:2982947; score == 68.7; expect == 5.e-11 *F and the predicted protein includes a 'Ribosomal_S15 domain' (IPR000589, *F PF00312) *F The GO terms for CG4207 include: *F \*F structural protein of ribosome ; GO:0003735 ; score == 54.7 | inferred *F from sequence similarity *F \*f mitochondrion ; GO:0005739 ; score == 54.7 | inferred from sequence *F similarity *F so I think that CG4207 and bonsai are the same gene, *F Gillian *F Browser: Mozilla/4.7 [en] (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131185 *a Ashburner *b M. *t 2000.8.5 *T personal communication to FlyBase *u FlyBase error report for CG15637 on Sat Aug 5 13:10:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Aug 05 21:04:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 5 Aug 2000 21:04:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 5 Aug 2000 13:10:42 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG15637 on Sat Aug 5 13:10:42 2000 *F Content-Length: 230 *F Error report from m.ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG15637 *F Missed gene *F Comments: This must be dumpy (dp). *F Browser: Mozilla/4.7 [en] (X11; I; SunOS 5.8 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131186 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG11173 on Thu Oct 26 00:04:59 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 08:06:24 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 08:06:24 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 00:04:59 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG11173 on Thu Oct 26 00:04:59 2000 *F Content-Length: 303 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG11173 *F Missed gene *F Comments: This is a homolog of the mammalian protein SNAP-29 and should be *F referred to as such. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131187 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG3279 on Thu Oct 26 00:45:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 08:45:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 08:45:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 00:45:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG3279 on Thu Oct 26 00:45:55 2000 *F Content-Length: 351 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG3279 *F Missed gene *F Comments: This gene was miscategorized as a motor protein. It is a SNARE with *F mammallian and yeast homolog. It should be referred to as vti1a. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131188 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG13626 on Thu Oct 26 00:49:07 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 08:49:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 08:49:53 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 00:49:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG13626 on Thu Oct 26 00:49:07 2000 *F Content-Length: 245 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG13626 *F Missed gene *F Comments: this should be syntaxin 18 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131189 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG1467 on Thu Oct 26 00:50:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 08:50:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 08:50:36 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 00:50:37 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG1467 on Thu Oct 26 00:50:37 2000 *F Content-Length: 243 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG1467 *F Missed gene *F Comments: this should be syntaxin 16 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131190 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG4109 on Thu Oct 26 00:57:56 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 08:58:00 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 08:58:00 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 00:57:57 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG4109 on Thu Oct 26 00:57:56 2000 *F Content-Length: 227 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG4109 *F Missed gene *F Comments: syntaxin 8 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131191 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG7736 on Thu Oct 26 01:00:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:00:21 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:00:21 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:00:20 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG7736 on Thu Oct 26 01:00:20 2000 *F Content-Length: 223 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG7736 *F Missed gene *F Comments: syn 6 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131192 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG1515 on Thu Oct 26 01:04:02 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:04:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:04:01 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:04:02 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG1515 on Thu Oct 26 01:04:02 2000 *F Content-Length: 221 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG1515 *F Missed gene *F Comments: ykt6 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131193 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG1599 on Thu Oct 26 01:05:17 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:05:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:05:20 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:05:18 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG1599 on Thu Oct 26 01:05:17 2000 *F Content-Length: 223 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG1599 *F Missed gene *F Comments: VAMP 7 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131194 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG7359 on Thu Oct 26 01:09:14 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:09:12 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:09:12 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:09:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG7359 on Thu Oct 26 01:09:14 2000 *F Content-Length: 223 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG7359 *F Missed gene *F Comments: sec22b *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131195 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG14084 on Thu Oct 26 01:11:09 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:11:08 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:11:08 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:11:09 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG14084 on Thu Oct 26 01:11:09 2000 *F Content-Length: 222 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG14084 *F Missed gene *F Comments: bet1 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131196 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG4780 on Thu Oct 26 01:25:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:25:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:25:57 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:25:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG4780 on Thu Oct 26 01:25:58 2000 *F Content-Length: 251 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG4780 *F Missed gene *F Comments: homolog of mammalian SNARE membrin *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131197 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG3539 on Thu Oct 26 01:22:55 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:34:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:34:37 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:22:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG3539 on Thu Oct 26 01:22:55 2000 *F Content-Length: 269 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG3539 *F Missed gene *F Comments: this is the fly homolog of sly1 in yeast and mammals *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131198 *a Courey *b A.J. *t 2000.6.28 *T personal communication to FlyBase *u *F From courey@chem.ucla.edu Wed Jun 28 18:42:14 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 28 Jun 2000 18:42:14 \+0100 *F Mime-Version: 1.0 *F Date: Wed, 28 Jun 2000 10:47:44 \-0700 *F To: flybase-help@morgan.harvard.edu *F From: Albert Courey *F Subject: HDAC4 *F Flybase lists two histone deacetylases in the 11E-12A region. One *F (CG1770) is based on the BDGP blast searches, while the other (HDAC4) *F appears to be based on an abstract from the last National fly meeting *F (Mallin et al. abstract \#203A). These seem to be one and the same *F gene. As far as I can tell, no cDNA sequences are available for HDAC4 *F and I can't find HDAC4 in the genomic sequence. CG1770 is highly *F homologous to human HDAC4. *F Al Courey *F ========================================================= *F Albert J. Courey *F UCLA *F Department of Chemistry and Biochemistry *F 405 Hilgard Avenue *F Los Angeles, CA 90095-1569 *F Phone: (310) 825-2530 *F FAX: (310) 206-4038 *F courey@chem.ucla.edu *F http://www.chem.ucla.edu/dept/Faculty/courey/index.html *F ======================================================== # *U FBrf0131199 *a Bornemann *b D. *t 2000.10.26 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Oct 26 09:50:34 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:50:34 \+0100 *F To: moconnor@mail.med.umn.edu *F Subject: FlyBase query: dHDAC4 *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 26 Oct 2000 09:50:34 \+0100 *F Content-Length: 1249 *F Dear Dr. O'Connor, *F I am a curator working for FlyBase. *F it was pointed out to us by a user, that the gene referred to as *F 'dHDAC4' in an abstract of yours may be the same as one of the *F predicted 'CG' genes in FlyBase. *F The abstract is: *F Mallin et al., 2000, A. Dros. Res. Conf. 41: 203A *F 'Genetic analysis of the Drosophila histone deacetylase dHDAC2.' *F In this, you state that 'dHDAC4' maps to 11F-12A. *F The predicted histone deacetylase is CG1770 (FBgn0030464), which is *F inferred to map to 11E1--3 from its position in the genomic sequence. *F This gene has homology to human 'histone deacetylase 4'. *F Do you know whether your 'dHDAC4' is the same as CG1770 \- if that is *F the case, then I can merge the two genes in the database (I would record *F this information as a personal communication from you to FlyBase), *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From dbornema@biosci.cbs.umn.edu Thu Oct 26 17:12:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 17:12:52 \+0100 *F Date: Thu, 26 Oct 2000 11:12:41 \-0500 (CDT) *F From: Doug Bornemann *F To: gm119@gen.cam.ac.uk *F Subject: HDAC4 *F MIME-Version: 1.0 *F Dear Gillian, *F My name is Doug Bornemann and I am working as a post doc in the O'Connor *F lab. You recently requested information about whether dHDAC4 on one of *F our abstracts corresponded with CG1770 which has homology to human HDAC4. *F Indeed our drosophila HDAC4 corresponds to CG1770, based on our sequencing *F of a dHDAC4 cDNA. The cDNA has some minor differences from the predicted *F sequence, and we are still working or resolving some ambiguities, which *F should be done soon. *F If you need any more information, let me know. *F Doug Bornemann # *U FBrf0131200 *a Wood *b V. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG6316 on Thu Oct 26 16:08:49 2000. *F >From val@sanger.ac.uk Thu Oct 26 16:08:43 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 16:08:43 \+0100 *F Sender: val@sanger.ac.uk *F Date: Thu, 26 Oct 2000 16:08:49 \+0100 *F From: Valerie Wood *F X-Mailer: Mozilla 4.7 [en] (X11; I; OSF1 V4.0 alpha) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: ma11@gen.cam.ac.uk *F CC: val@sanger.ac.uk *F Subject: gp error *F Content-Transfer-Encoding: 7bit *F Hi Michael, *F It looks like your CG6316 could be a a fusion of 2 proteins- see *F alignment of pombe SABC18A8.09 with human and drosophilla homologs *F below. Could you forward it to the appropriate person? *F cheers *F Val *F Q9Y5U9 HSPC039 PROTEIN. (82 aa) *F initn: 203 init1: 203 opt: 227 z-score: 334.4 E(): 3.7e-11 *F Smith-Waterman score: 227; 45.7% identity in 81 aa overlap *F 10 20 30 40 50 *F ;ID sp MFGFGNILYVTLLLLNAVAILSEDRFLGRIGWSQSAALG-FGDRQDTIKSRILHLIRAI *F : . ..: ..X: .::.:.: :.::: :::. . ..: ::. . :::....:::.. *F Q9Y5U9 MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGE-EPGIKSQLMNLIRSV *F 10 20 30 40 50 *F 60 70 80 *F ;ID sp RTVMTFPLIAINTIVIVYNLVLG *F :::: ::: .:.:.:X :..: *F Q9Y5U9 RTVMRVPLIIVNSIAIVLLLLFG *F 60 70 80 *F Q9VTE1 CG6316 PROTEIN. (550 aa) *F initn: 132 init1: 100 opt: 145 z-score: 208.1 E(): 0.0004 *F Smith-Waterman score: 145; 44.6% identity in 56 aa overlap *F 10 20 30 40 50 *F ;ID sp MFGFGNILYVTLLLLNAVAILSEDRFLGRIGWS-QSAALGFGDRQDTIKSRILHLI *F : ..::. :.. :: : :...:.X: *F Q9VTE1 EESNNLDRLERFLEQAAQSKEHTSDWGVWKLDKLGWGRQAGQQDFG--APTAKDQVLNLI *F 470 480 490 500 510 520 *F 60 70 80 *F ;ID sp RAIRTVMTFPLIAINTIVIVYNLVLG *F :.:::: .::: .: :.:...:.:X *F Q9VTE1 RSIRTVAKIPLIFLNIIAIIFKLLLG *F 530 540 550 *F \-- *F \------------------------------------------------------------------------------ *F ---- *F Valerie Wood Tel: 01223 494954 *F S. Pombe Genome Project Fax: 01223 494919 *F The Sanger Centre email: val@sanger.ac.uk *F Wellcome Trust Genome Campus http://www.sanger.ac.uk/Projects/S_pombe *F Cambridge *F CB10 1SA # *U FBrf0131201 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG8228 on Thu Oct 26 01:19:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:19:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:19:59 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:19:58 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG8228 on Thu Oct 26 01:19:58 2000 *F Content-Length: 237 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG8228 *F Missed gene *F Comments: fly homolog of vps45 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131202 *a Bock *b J. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG5127 on Thu Oct 26 01:24:44 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 09:24:44 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 09:24:44 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 01:24:44 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbbock@leland.stanford.edu *F Subject: FlyBase error report for CG5127 on Thu Oct 26 01:24:44 2000 *F Content-Length: 260 *F Error report from jason bock (jbbock@leland.stanford.edu) *F Gene or accession: CG5127 *F Missed gene *F Comments: homolog of yeast vps33 and mammalian vps33b *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131203 *a Cohen *b S. *t 2000.10.26 *T personal communication to FlyBase *u FlyBase error report for CG5031 on Thu Oct 26 02:34:41 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Oct 26 10:34:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Oct 2000 10:34:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Oct 2000 02:34:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: cohen@embl-heidleberg.de *F Subject: FlyBase error report for CG5031 on Thu Oct 26 02:34:41 2000 *F Content-Length: 329 *F Error report from stephen cohen (cohen@embl-heidleberg.de) *F Gene or accession: CG5031 *F cDNA or EST error *F Comments: CG5031 does not correspond to LD47466 as is presently indicated. *F It does match CK00242, though this is incomplete. *F Browser: Mozilla/4.08 (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131204 *a Levis *b R. *t 2000.10.25 *T personal communication to FlyBase *u FlyBase error report for CG3766 on Wed Oct 25 13:51:27 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Oct 25 23:54:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Oct 2000 23:54:54 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Oct 2000 13:51:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG3766 on Wed Oct 25 13:51:27 2000 *F Content-Length: 442 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG3766 *F Gene annotation error *F Gene CG3766 corresponds to FBgn0011232 *F Comments: The P element insertion ms(2)05289 is within the predicted exon of *F CG3766. This P insertion has been verified to be associated with a male *F sterile allele of the gene scattered (scat). *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0131205 *a Mount *b S. *t 2000.10.26 *T personal communication to FlyBase *u *F From smount@wam.umd.edu Fri Oct 27 01:00:06 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 27 Oct 2000 01:00:06 \+0100 *F X-Authentication-Warning: rac2.wam.umd.edu: smount owned process doing \-bs *F Date: Thu, 26 Oct 2000 20:00:07 \-0400 (EDT) *F From: Stephen M Mount *F To: flybase-help@morgan.harvard.edu *F Subject: Gene Hrb85CD *F MIME-Version: 1.0 *F Hi! *F The gene Hrb85CD gene does not exist. It was a mistaken name for *F Hrb87F/hrp36. You can easily verify this by blast searches, etc.. I *F pointed this out at the Jamboree, and there is no Hrb85CD in GadFly (which *F is a good thing). Do you think there is room for a new category: you could *F have separate lists for synonyms and misomers! *F Steve Mount *F \################################################## *F Stephen M. Mount *F Cell Biology and Molecular Genetics *F H. J. Patterson Hall *F University of Maryland *F College Park, MD 20742-5815 *F Phone 301-405-6934 *F FAX 301-314-9081 *F URL http://www.wam.umd.edu/~smount/ *F email sm193@umail.umd.edu *F \################################################## # *U FBrf0131206 *a Robertson *b H. *t 2000.10.17 *T personal communication to FlyBase *u FlyBase error report for CG18859 on Tue Oct 17 11:55:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 17 19:55:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Oct 2000 19:55:46 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 17 Oct 2000 11:55:39 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG18859 on Tue Oct 17 11:55:39 2000 *F Content-Length: 1556 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG18859 *F Gene annotation error *F Gene CG18859 has incorrect exon/intron structure. *F Comments: A different C-terminus is required to align well with related *F odorant receptors, and this protein is Or19a. My cDNA is *F ATGGACATATCGAAGGTGGATTCAACGAGGGCTCTGGTTAACCACTGGCGCATCTTCAGGATTATGGGAATCCATCCGCC *F GGGCAAGAGGACCTTCTGGGGTCGCCACTACACGGCGTACTCCATGGTGTGGAACGTAACCTTCCACATCTGCATCTGGG *F TGTCCTTTTCGGTCAATCTCCTGCAGTCCAATTCGCTGGAGACTTTCTGCGAGAGCCTCTGCGTGACCATGCCGCACACG *F CTCTACATGCTTAAGCTGATCAATGTCCGTCGGATGCGCGGCCAGATGATCAGCAGCCACTGGTTGCTCCGTCTCTTGGA *F CAAGCGGCTTGGCTGCGACGACGAACGCCAGATCATTATGGCCGGCATCGAGCGGGCCGAGTTCATATTCCGCACCATTT *F TTCGCGGCCTTGCGTGCACCGTCGTCCTTGGCATCATCTACATATCCGCGTCCAGCGAGCCCACGCTGATGTACCCCACC *F TGGATTCCCTGGAACTGGAGGGACAGCACCTCCGCCTACCTGGCCACCGCCATGCTGCACACGACCGCCCTCATGGCGAA *F TGCGACACTTGTCCTCAATCTGAGCTCCTATCCGGGCACCTACCTCATCCTGGTCAGTGTCCACACCAAGGCGCTCGCCC *F TGCGGGTCTCCAAATTGGGATATGGCGCGCCACTACCGGCGGTTCGGATGCAGGCCATTCTGGTTGGTTACATCCACGAC *F CACCAGATCATTTTGCGCCTCTTCAAGTCACTGGAGAGATCCCTTTCGATGACCTGCTTTCTGCAGTTCTTCAGCACGGC *F GTGTGCGCAGTGCACAATCTGCTACTTTCTACTCTTCGGGAACGTCGGGATCATGAGGTTCATGAATATGTTGTTCCTGC *F TGGTGATCCTCACCACGGAGACCCTTCTTCTCTGCTACACGGCGGAGCTACCTTGCAAGGAAGGGGAGAGCCTCCTGACC *F GCTGTCTACAGCTGCAACTGGCTGTCCCAGTCGGTAAACTTTCGGAGACTCCTGCTCCTGATGCTCGCACGCTGCCAGAT *F TCCAATGATCCTGGTCTCCGGCGTAATTGTGCCCATCAGCATGAAGACCTTCACGGTGATGATTAAGGGAGCGTACACCA *F TGCTTACTCTGCTGAATGAAATTCGTAAAACGTCCCTTGAATAG *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0131207 *a Ashburner *b M. *t 2000.10.30 *T personal communication to FlyBase *u FlyBase error report on Mon Oct 30 11:01:07 2000. *F From ma11 Mon Oct 30 09:43:18 2000 *F To: ma11@gen.cam.ac.uk, ag24@gen.cam.ac.uk *F Subject: FlyBase sequence errors for Heat shock Proteins 70 *F X-Sun-Charset: US-ASCII *F Content-Length: 1008 *F X-Lines: 33 *F Aubrey pointed this out. I replied that I was by no means surprised since *F the assembly algorithm is really unhappy with low copy-number repeats *F as are at both Hsp70A and Hsp70B. *F But I did a leetle investigation of v1.0 proteins: *F These are probably bits of Hsp70B (at 87C): *F >CG6489|FBan0006489|CT20131|FBan0006489 last_updated:000321 *F EKHRQRIPSRNALESYVFNVKQSVEQAPAGKLDEADKNSVLDKCNETIRWLDSNTTAEKE *F EFDHKMEELTRHCSPIMTKMHQQGAGAAGGPGANCGQQAGGFGGYSGPTVEEVD *F >CG5834|FBan0005834|CT18301|FBan0005834 last_updated:000321 *F MHQQGAGAAGGPGANCGQQAGGFGGYSGPTVEEITDYKMEPPFAPLSESCTKALGDKVYE *F KRKLASQEIRHPNWEM *F Michael # *U FBrf0131208 *a Maier *b D. *t 2000.8.3 *T personal communication to FlyBase *u *F From gopher@firefly.bio.indiana.edu Wed Aug 02 16:08:43 2000 *F From: fbmailer@bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail *F comments: Please, note that the Hairless def Df(3R)H89c.27 is no longer *F available. Since it is still in your list I get many of requests. *F It would be nice if you could correct this in the flybase. *F Many thanks *F Dieter Maier *F Dieter Maier *F maierdie@uni-hohenheim.de # *U FBrf0131209 *a Brill *b J. *t 2000.8.14 *T personal communication to FlyBase *u *F Archived. # *U FBrf0131210 *a Akam *b M. *t 1996.1.8 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Aug 22 00:48:12 2000 *F Subject: pc from Michael Akam *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Michael Akam, Wellcome/CRC Institute *F To: Bloomington Drosophila Stock Center *F Subject: UAS Ubx1A *F Dated: 8 January 1996 *F Note from Bloomington: This insertion is now known to FlyBase as *F P{UAS-Ubx.Ia.C}36.2. *F Information communicated: *F PUAS Ubx1A, w+36.2 is a homozygous viable insertion at 96D. # *U FBrf0131211 *a Perrimon *b N. *t 1995.5.3 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Aug 22 00:51:59 2000 *F Subject: pc from Norbert Perrimon *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Norbert Perrimon, Harvard University *F To: Bloomington Drosophila Stock Center *F Subject: FRT9.2 *F Dated: 3 May 1995 *F Note from Bloomington: This insertion is now known to FlyBase as *F P{FRT(whs)}9-2. *F Information communicated: *F P[mini w+;FRT][9-2] is a viable insertion at 18E. # *U FBrf0131212 *a Brown *b N. *t 1998.10.22 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Aug 22 00:56:00 2000 *F Subject: pc from Nicholas Brown *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Nicholas Brown, Wellcome/CRC Institute *F To: Bloomington Drosophila Stock Center *F Subject: GAL4 line 48Y *F Dated: 22 October 1998 *F Note from Bloomington: This insertion is now known to FlyBase as P{GawB}48Y. *F Information communicated: *F P[w+, GAL4] line 48Y is a viable insertion in 26C. It was mapped by in situ *F hybridisation. # *U FBrf0131213 *a Paloma Martin *b M. *t 1997.1.20 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Aug 22 01:06:29 2000 *F Subject: pc from M. Paloma Martin *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: M. Paloma Martin, Universidad Autonoma de *F Madrid, stock-keeper of Antonio Garcia-Bellido's lab *F To: Bloomington Drosophila Stock Center *F Subject: P{f+}37C *F Dated: 20 January 1997 *F Note from Bloomington: This insertion is now known to FlyBase as *F P{f+13}37C. *F Information communicated: *F P{f+}37C is an insertion in 37C. The chromosome is lethal. # *U FBrf0131214 *a Padgett *b R. *t 2000.8.24 *T personal communication to FlyBase *u *F X-Sender: padgett@waksman.rutgers.edu (Unverified) *F Date: Thu, 24 Aug 2000 13:12:27 \-0400 *F To: Leyla Bayraktaroglu *F From: 'Richard W. Padgett' *F Subject: Re: the tmp ('temporarily deranged') gene *F yes, it corresponds to dSmad2. *F >Dear Dr. Padgett, *F > *F >I am a molecular curator at the FlyBase-Harvard site. *F >I recently came across a FlyBase gene named 'tmp', curated *F >from an abstract from your lab from the 1998 Drosophila *F >Research Conference. I was wondering if tmp corresponds *F >to Smox (aka dSmad2). *F > *F >Thanks very much for your time. *F > *F >Leyla Bayraktaroglu *F >Curator, FlyBase *F \--------------------------------------------------------------------------- *F Richard W. Padgett *F Waksman Institute *F 190 Frelinghuysen Road *F Rutgers University *F Piscataway, NJ 08854-8020 *F padgett@waksman.rutgers.edu *F office:732-445-0251 *F FAX: 732-445-5735 *F http://waksman.rutgers.edu/padgett # *U FBrf0131215 *a Bellen *b H. *t 2000.8.28 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Mon Aug 28 17:00:24 2000 *F Subject: Df(2L)spdj2 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Hugo Bellen, HHMI/Baylor College of Medicine *F To: Bloomington Drosophila Stock Center *F Subject: Df(2L)spdj2 *F Dated: 28 August 2000 *F Information communicated: *F Nrv1 and Nrv2 are complemented by Df(2L)spdj2. # *U FBrf0131216 *a Kennison *b J. *t 1993.7.1 *T personal communication to FlyBase *u *F >From matthewk@mail-relay.indiana.edu Mon Sep 04 23:31:30 2000 *F Subject: complementation data *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Jim Kennison, National Institutes of Health *F To: Bloomington Drosophila Stock Center *F Subject: complementation data *F Dated: 1 July 1993 *F Information communicated: *F l(3)neo461 complements Df(3R)sbd105 *F l(3)ry1191 complements Df(3L)kto2 # *U FBrf0131217 *a Wasserman *b S. *t 1991.4.1 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Fri Sep 15 18:48:19 2000 *F Subject: Df(3L)GN19 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Steven Wasserman, U.T. Southwestern Medical Center *F To: Bloomington Drosophila Stock Center *F Subject: Df(3L)GN19 *F Dated: 1 April 1991 *F Information communicated: *F Df(3L)GN19 was generated by gamma-irradiation of P{lacW}Y392. # *U FBrf0131218 *a Verstreken *b P. *c H. *d Bellen *t 2000.9.4 *T personal communication to FlyBase *u *F Archived. # *U FBrf0131219 *a Cook *b K. *t 2000.10.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Oct 09 20:29:21 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{PZ}TlrK344 *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{PZ}TlrK344 *F The proximity of the P{PZ} insertion in the 'l(3)rK344' line (FBti0009958) *F to the Toll gene (BDGP data) suggested that it was a mutant Tl *F allele. Complementation tests against Df(3R)Tl-X, Df(3R)Tl-P, Df(3R)Tl-I *F and Df(3R)ME61 showed no lethality; however, females laid eggs that did not *F hatch. Complementation tests against Tlr3 and Tlr4 also produced *F sterile females. Examination of eggs from the Tlr3 complementation test *F showed dorsalized embryos. Consequently, the genotype of the 'l(3)rK344' *F chromosome should be changed from P{PZ}l(2)rK344rK344 to P{PZ}TlrK344, *F l(3)rK344rK344 to indicate that the P insertion is associated with Tl and *F that the lethality is not associated with the P insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0131220 *a Rees *b D. *t 1990.8.1 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Mon Oct 09 22:25:16 2000 *F Subject: vnc alleles *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Dianne Rees, Indiana University *F Subject: vnc alleles *F Dated: 1 August 1990 *F Background: The following information from Dianne Rees's Ph.D. *F thesis (FBrf0070514) describes alleles she isolated in a screen for *F female sterile mutation in the 67A \-- 67D interval. All *F observations included here are Dianne's. *F Information communicated: *F gene: *F vnc, variable nurse cells *F map location: *F 67B \-- 67D based on deficiency complementation; maps between h *F and th by recombination *F complementation: *F fails to complement Df(3L)AC1 *F complements Df(3L)29A6 *F complements RpS174 *F alleles: *F vnc2 and vnc14 *F allele class: *F hypomorphic, vnc2 slightly stronger than vnc14 *F phenotype: *F viable, female sterile *F The phenotypes associated with these mutations implicate the vnc *F product in the control of cystocyte divisions within the *F germarium. *F Hemizygous vnc2/Df(3L)AC1, vnc14/Df(3L)AC1 and *F homozygous vnc2 females produce no eggs. Homozygous vnc14 *F females produce a few flaccid eggs with abnormal chorions *F (abnormal numbers of chorionic filaments and thin chorionic *F membranes). vnc2/vnc14 heterozygotes produce eggs. *F Hemizygous vnc2/Df(3L)AC1 and vnc14/Df(3L)AC1 ovaries are *F small and comprised of tumorous egg chambers. Nuclei within these *F egg chambers appear undifferentiated. Homozygous vnc2 and *F vnc14 egg chambers range from too few through normal numbers to *F too many nurse cells. *F other info: *F X-ray-induced on kniri-1 e1. vnc2 and vnc14 were among 15 *F female sterile alleles of this gene recovered in a screen of *F 3,000 chromosomes (the other 13 alleles have been lost). No *F alleles of vnc were recovered in screens of 8,750 EMS-treated and *F 3,000 DEB-treated kniri-1 e1 chromosomes, raising the *F possibility that this gene is sensitive to X-rays. In all cases *F mutations were identified by their failure to complement *F Df(3L)AC1 for female fertility. Differences in oogenic defects *F among alleles argues against the 15 vnc alleles arising as a *F cluster from a spontaneous premeiotic event. *F Four additional female-sterile loci in the 67B \-- 67D interval (in *F addition to alphaTub67C) were identified in these screens, but all *F alleles of those four genes have been lost. # *U FBrf0131221 *a Freeman *b M. *c A. *d Dobritsa *e J. *f Carlson *t 2000.10.26 *T personal communication to FlyBase *u *F The following information was supplied to FlyBase by Marc Freeman, Anna *F Dobritsa and John Carlson in response to a molecular query about the dare gene *F by a FlyBase curator for use in genome annotation. *F *F 1. dare[1] *F Does the start of accession AF168685 correspond to the P *F insertion site in dare[1]? *F *F **Yes. We isolated a number of dare cDNAs from a Drosophila head cDNA *F library with 5' ends that mapped in genomic DNA 3' of the dare1 P insertion *F site. To determine the true 5' end of the dare transcript (or attempt to), *F we performed 5' RACE and obtained a number of products: the 5' end of some *F of these mapped to genomic DNA 5' of the dare^1 P insertion site, while *F others mapped 3' to the P insertion. EST LD17269 does indeed map 14 bp 5' *F of the P insertion site, and is the longest 5' end from any dare cDNAs, or *F RACE products. At present it is not clear if this is the true 5' end, or *F if the length of the 5' end of the dare transcript is heterogeneous, with *F LD17269 being the longest version isolated to date. *F *F 2. dare[34] *F Does the 3' deletion endpoint of dare[34] map to a 0.8 kb *F ClaI fragment? *F *F **Yes. As far as we could tell. *F *F 3. dare[5] *F Does the 3' deletion endpoint of dare[5] map to a 1.95 kb *F PstI fragment? *F *F **Yes. This is as precisely as we have defined it to date. *F *F 4. dare[4] *F What is the 3' deletion endpoint of dare[4]? The P insertion *F site of dare[1]? *F *F **We know that the 3' end of the PlacW element is present in dare^4 based *F on Southern analysis. Everything in the genomic region 3' of the P *F insertion *F appears to be intact. We do not know how much of the PlacW element is left *F in dare^4, although the 5'-most end of the P insertion appears to be gone. *F Our only other information is that the mini-white+ marker has been lost *F from PlacW. *F *F We also note that, the 5' end of the dare4 and dare5 deletions have not *F been determined. *F *F 5. dare[102] *F The start of the dare[102] deletion is said to begin 8 bp 3' *F of the P insertion site. Do you have a precise endpoint? *F *F ** Yes, we do. The sequence is intact up till the 9th bp after the dare^1 *F P insertion site. That is the sequence corresponding to the first eight *F nts in AF168685 is present in dare^102, then there is a 2054 bp deletion *F that removes the entire dare coding region (3' breakpoint corresponds to *F the nt 1562 in AF168685). Also, there is a 25 bp insertion of what *F appears to be a piece of the inverted repeat of the P element left *F behind (between nt 8 and nt 1562). *F *F 6. rescue construct "GF-AR" *F Do the restriction sites of the 6.5 kb BamHI-ClaI rescue *F fragment correspond to the first BamHI site and second ClaI site on *F the map in figure 2A? (I realize that a 0.5 kb SacI fragment was *F removed too.) *F *F **Referring to figure 2A: GF-AR begins at the second (not the first) BamHI *F site, and extends to the second (as you stated above) ClaI site. *F *F 7. dare transcript *F The first intron does not seem to be spliced out of EST *F LD17269: do you think it is simply an unprocessed transcript? *F *F **We have sequenced across the first intron in genomic DNA, as well as two *F independently isolated cDNAs. Both show the same splicing pattern as diagrammed *F in Fig. 2A and indicated by the cDNA sequence (AF168685) when it is compared *F to genomic DNA. The protein product predicted from this splicing pattern aligns *F very well with bovine, human, and yeast AR, and is therefore likely to be correct. *F (We also rescued many phenotypes with a hs-AR construct that had a fragment *F corresponding to AF168685.) # *U FBrf0131222 *a Lehmann *b R. *c M. *d Starz-Gaiano *t 2000.11.28 *T personal communication to FlyBase *u *F From Ruth Lehmann and Michelle Starz-Gaiano *F *F Date: Mon, 27 Nov 2000 16:32:57 -0400 *F To: leyla@morgan.harvard.edu *F From: Ruth Lehmann *F Subject: Re: wun2 question (fwd) *F Cc: starz@mcbi-34.med.nyu.edu *F *F Dear Leyla, *F *F Thank you for your inquiry into tunen/wunen-2. They are the same gene, *F although we have about 14 bases more at the 5' end in wunen-2 than in *F tunen, and a few other polymorphisms. We plan to submit this *F sequence to genbank. Thus, our clone maps to 101175 on the AE003833(.2) *F sequence and is longer than CG8805 as you mentioned. We would like *F to suggest that you choose wunen-2 as the name for the gene (CG8805), in *F order to make the similarity between the genes very clear. Here are our *F observations: *F 1) We have shown that ALL original wunen mutations described by Howard and *F colleagues, delete both genes. Thus at this point the most likely *F explanation is that wunen and wunen-2 are functionally redundant. *F Additional evidence supporting this notion are: *F 2) Both genes are expressed in the same pattern. *F 3) Both genes when misexpressed and overexpressed have the identical effect *F on germ cells. *F *F A manuscript describing this work from our lab has been accepted by *F Development. We think the name Tunen is confusing and was put into the *F database without any publication backing up any of the data. Furthermore, *F there are 6 additional wunen homologs in the data base and it may be a good idea to *F give them a uniform name (such as wunen-3, wunen-4 etc rather than *F thrunen? and fournen etc. *F We think for once fly nomenclature should try to simplify rather than *F further complicate already confusing data. *F *F Thank you very much and we would welcome further discussion of this issue. *F Best regards, *F Ruth Lehmann and Michelle Starz-Gaiano. *F *F >> *F >> > ---------- Forwarded message ---------- *F >> > Date: Mon, 20 Nov 2000 16:24:31 -0500 (EST) *F >> > From: Leyla Bayraktaroglu *F >> > To: starz@mcbi-34.med.nyu.edu *F >> > Subject: wun2 question *F >> > *F >> > Dear Dr. Starz-Gaiano, *F >> > *F >> > I am a molecular curator at FlyBase. *F >> > One of our current tasks is to try and match "known" *F >> > fly genes to the ones predicted from the Celera/BDGP *F >> > sequence. *F >> > *F >> > >From the available evidence, it looks like Tunen and *F >> > wun2 are the same thing. I wanted to double check this *F >> > with you, since I don't have any sequence information *F >> > on wun2 but thought that you might. *F >> > *F >> > In a personal communication to Rachel Drysdale, *F >> > you stated that "... wunen 2 is NOT l(2)k10201; wun2 lies *F >> > on the other side of wunen, (about 4kb away) and they are *F >> > transcribed away from each other." *F >> > The wun-like phosphatidate phosphatase Tunen (CG8805) is 4.933 kb *F >> > away from wun, and transcribed away from it. *F >> > (Celera annotation has missed the first exon of Tunen, *F >> > which should start at coordinate 101189 of GenBank accession *F >> > AE003833 rather than 103446). *F >> > *F >> > gene order annotated on Celera/BDGP sequence is: *F >> > *F >> > l(2)k10201 wun Tunen. *F >> > *F >> > *F >> > Thanks very much for your time. *F >> > *F >> > Leyla Bayraktaroglu *F >> > Curator, FlyBase *F >> # *U FBrf0131225 *a Bayraktaroglu *b L. *t 2000.11.2 *T personal communication to FlyBase *u *F From leyla@morgan.harvard.edu Thu Nov 02 01:19:12 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Thu, 2 Nov 2000 01:19:12 +0000 *F Date: Wed, 1 Nov 2000 20:18:24 -0500 (EST) *F From: Leyla Bayraktaroglu *F Reply-To: Leyla Bayraktaroglu *F Subject: Current list of possibly CG-able known genes *F To: ag24@gen.cam.ac.uk *F Cc: curators@morgan.harvard.edu, joel@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-Type: MULTIPART/mixed; BOUNDARY='Corps_of_Giraffes_186_000' *F X-Mailer: dtmail 1.2.1 CDE Version 1.2.1 SunOS 5.6 sun4u sparc *F Content-Length: 23749 *F *F Hi Aubrey, *F *F Attached are the CG-to-known gene correspondences for some *F of the genes in the nc4 and nc6 categories. *F *F nc4: genes with no SWP/TREMBL/PIR entry or protein_id but which look *F like ESTs (428). *F *F nc6: everything else with a nucleic acid entry (115). *F *F *F Joe Lemaire did batch BLAST of accessions attached to the gene *F against predicted transcripts (CTs) and Celera/BDGP scaffolds, *F and I scanned the output. Additional alignments were performed *F as necessary. *F *F Also ttached are the few genes that I have been able to *F resolve from the nc7 file. *F *F nc7: genes with no nucleic acid or protein accessions but which still *F feature in 'gene order' statements in the genes data (548). *F *F These fall into 2 categories: *F *F 1.sequence data was in paper but not in the sequence databanks *F (very straightforward) (nc7_done) *F *F 2. there was detailed molecular information that made it *F easy to place the genes. These are marked with 'curator inference' *F (nc7_inference). *F *F anon-EST:CL1c2 RhoL (CG9366|FBan0009366|CT26615|) *F *F anon-EST:CL2c12 Act57B (CG10067|FBan0010067|CT28343|) *F *F anon-EST:CL2d4 CG4677|FBan0004677|CT15069| *F *F anon-EST:CL32 CadN (CG7100|FBan0007100|CT21941|) 3'UTR *F *F anon-EST:CL57 CG-less at AE003457:118582..118646,118706..118748 *F between CG6044 and qkr58E-3 *F *F anon-EST:fe1A1 mt:lrRNA *F *F Query= gi|2130664|gb|AA433202.1|AA433202 EST1 Drosophila *F melanogaster Uni-ZAP XR library (Stratagene cat.#937602) Drosophila *F melanogaster cDNA clone 1A1 5'. *F (190 letters) *F *F >emb|X53506.1|DM16SR Drosophila mRNA to mitochondrial 16S ribosomal RNA gene *F Length = 1324 *F Score = 232 bits (117), Expect = 4e-59 *F Identities = 155/164 (94%), Gaps = 3/164 (1%) *F *F anon-EST:fe1A11 CG-less at AE003558:101280..101518 *F *F anon-EST:fe1A12 aligns to several scaffolds: repeat? *F *F anon-EST:fe1A2 CG7808|FBan0007808|CT5020| *F *F anon-EST:fe1A5 CG1475|FBan0001475|CT2508| (anon-EST:Posey125) *F *F anon-EST:fe1A7 CG7971|FBan0007971|CT6255| *F transcript structure to be fixed *F _______________________________________________________________________________ *F anon-EST:fe1B1 mt:Cyt-b *F *F BLAST of AA433193 against AF200828 *F *F Score = 360 bits (187), Expect = 5e-98 *F Identities = 245/262 (93%), Positives = 245/262 (93%), Gaps = 8/262 (3%) *F Query: 1 agctccaattaatatt--aagatnnnngaaattttngatcattacttggattatgtttaa 58 *F |||||||||||||||| ||||| |||||||| |||||||||||||||||||||||| *F Sbjct: 10557 agctccaattaatatttcaagatgat-gaaattttggatcattacttggattatgtttaa 10615 *F cytochrome b 24 A P I N I S S W W N F G S L L G L C L *F *F *F Query: 59 ttattcaaattttaaccggattatttttagctatacattacacagctgatattaatctag 118 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 10616 ttattcaaattttaaccggattatttttagctatacattacacagctgatattaatctag 10675 *F cytochrome b 43 I I Q I L T G L F L A M H Y T A D I N L *F *F *F Query: 119 ctttctatagtgttaatcatatttgtcgaaacgttaattatggttgattattacgaactt 178 *F ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 10676 ctttctatagtgttaatcatatttgtcgagacgttaattatggttgattattacgaactt 10735 *F cytochrome b 63 A F Y S V N H I C R D V N Y G W L L R T *F *F *F Query: 179 tatatgctaa-ggtgcatca--ttttcnnatttgtatttacttacatgtaggacgagg-a 234 *F || ||||||| ||||||||| |||| ||||||||||||||||||||||||||||| | *F Sbjct: 10736 tacatgctaacggtgcatcatttttttttatttgtatttacttacatgtaggacgaggaa 10795 *F cytochrome b 83 L H A N G A S F F F I C I Y L H V G R G *F *F *F Query: 235 tttattacgg-tcatataaatt 255 *F |||||||||| ||||||||||| *F Sbjct: 10796 tttattacggttcatataaatt 10817 *F cytochrome b 103 I Y Y G S Y K F *F *F _______________________________________________________________________ *F anon-EST:fe1B12 dpn (CG8704|FBan0008704|CT3681|) *F *F anon-EST:fe1B5 CG-less at AE003622:192526..192731 *F *F anon-EST:fe1B9 matches CG-less at AE003502:117020..117354 *F *F anon-EST:fe1C10 CG9354|FBan0009354|CT25494| *F *F anon-EST:fe1C12 CG4111|FBan0004111|CT13646| *F *F anon-EST:fe1C6 matches CG-les at AE003578:233366..233808 *F *F anon-EST:fe1D1 RpL13 (CG4651|FBan0004651|CT15029|) *F *F anon-EST:fe1D11 CG8589|FBan0008589|CT24993| *F *F anon-EST:fe1D11 CG8589|FBan0008589|CT24993| *F *F anon-EST:fe1D2 CG8857|FBan0008857|CT25442| *F *F anon-EST:fe1D4 CG10652|FBan0010652|CT29824| *F *F anon-EST:fe1E12 CG16912|FBan0016912|CT10134| *F *F anon-EST:fe1E4 CG-less at AE003790:179593..179374,171576..171357 *F *F anon-EST:fe1E6 CG3329|FBan0003329|CT10524| *F *F anon-EST:fe1E8 CG9354|FBan0009354|CT25494| *F *F anon-EST:fe1F11 CG-less at AE003455:29428..29123 *F *F anon-EST:fe1G6 CG3329|FBan0003329|CT10524| *F *F anon-EST:fe1G7 mbt (CG18582|FBan0018582|CT14490|) *F (AA433228 aligned to mbt) *F alignment with mbt acc. AJ011578 extends 3' end of *F last exon of mbt on AE003502 to 279638 *F *F anon-EST:fe1H3 CG8933|FBan0008933|CT25652|CT41767 *F *F anon-EST:fe1H6 CG5802|FBan0005802|CT18200| *F *F anon-EST:fe2A1 CG5820|FBan0005820|CT18251| *F *F anon-EST:fe2A11 CG6605|FBan0006605|CT20532| *F *F anon-EST:fe2A3 CG10305|FBan0010305|CT28945| *F *F MERGE: *F *F anon-EST:fe2A9 CG17467|FBan0017467|CT38621| (AA433262 aligned to AA433242) *F anon-EST:fe2E7 CG17467|FBan0017467|CT38621| *F *F anon-EST:fe2B1 trn (CG11280|FBan0011280|CT31487|) *F *F anon-EST:fe2B11 CG-less at AE003767:46459..46126 *F *F anon-EST:fe2B3 CG-less at AE003484:50065..49882 *F *F anon-EST:fe2B6 CG3800|FBan0003800|CT12687| *F *F anon-EST:fe2D1 RpL23a (CG7977|FBan0007977|CT6274|) *F *F *F MERGE: *F anon-EST:fe2D10 CG3773|FBan0003773|CT12578| (AA433250 aligned to AE003725) *F anon-EST:fe2D12 CG3773|FBan0003773|CT12578| (AA433251 aligned to AA433250) *F *F *F anon-EST:fe2D3 RpS18 (CG8900|FBan0008900|CT25560|) *F *F anon-EST:fe2E12 Trl (CG9343|FBan0009343|CT40931| CT26545|) *F *F anon-EST:fe2E2 alpha-Spec (CG1977|FBan0001977|CT6173|) *F *F anon-EST:fe2E3 CG10712|FBan0010712|CT30015| *F *F anon-EST:fe2E5 CG16803|FBan0016803|CT40743||CT34211| *F *F anon-EST:fe2E7 CG17467|FBan0017467|CT38621| *F *F anon-EST:fe2G4 CG1935|FBan0001935|CT5993| *F *F anon-EST:fe2H10 CG3186|FBan0003186|CT10685| *F *F anon-EST:fe2H6 CG7839|FBan0007839|CT23793| *F *F anon-EST:fe2H7 sina (CG9949|FBan0009949|CT28005|) *F *F anon-EST:fe3A1 CG2249|FBan0002249|CT7468| *F *F anon-EST:fe3A6 RpL9 (CG6141|FBan0006141|CT37413|) *F *F anon-EST:fe3B12 CG12859|FBan0012859|CT31999| *F *F anon-EST:fe3B4 CG8495|FBan0008495|CT24837| *F *F anon-EST:fe3C6 CG9177|FBan0009177|CT26238|CT41796 *F *F anon-EST:fe3C7 CG7088|FBan0007088|CT21889| *F *F anon-EST:fe3C9 Khc-73 (CG8183|FBan0008183|CT21931|) *F *F anon-EST:fe3D10 Ance (CG8827|FBan0008827|CT25364|) *F *F anon-EST:fe3D3 CG5738|FBan0005738|CT18040| *F *F anon-EST:fe3D7 Crc (CG9429|FBan0009429|CT26738|) *F *F anon-EST:fe3D9 CG8983|FBan0008983|CT25820| *F *F anon-EST:Liang-1.1 SsRbeta (CG5474|FBan0005474|CT17318|) *F *F anon-EST:Liang-2.38 CG6876|FBan0006876|CT21288| *F *F anon-EST:Liang-2.44 CG17836|FBan0017836|CT39616| *F *F anon-EST:Liang-2.45 CG7546|FBan0007546|CT15742| *F *F anon-EST:ParkEST009 CG8399|FBan0008399|CT18617| *F *F anon-EST:ParkEST060 CG7532|FBan0007532|CT23067| *F *F anon-EST:ParkEST066 CG7714|FBan0007714|CT21023| *F *F anon-EST:ParkEST107 CG2297|FBan0002297|CT7648| *F *F anon-EST:ParkEST130 CG9496|FBan0009496|CT26888| *F *F anon-EST:ParkEST153 CG9675|FBan0009675|CT27358| *F *F anon-EST:ParkEST161 graal (CG4948|FBan0004948|CT15880|) *F *F anon-EST:ParkEST167 CG7658|FBan0007658|CT23381| *F *F anon-EST:ParkEST180 Gasp (CG10287|FBan0010287|CT28895|) *F *F anon-EST:ParkEST188 CG7013|FBan0007013|CT21690| *F *F anon-EST:ParkEST240 CG7663|FBan0007663|CT23427| *F *F anon-EST:ParkEST264 Fer2LCH (CG1469|FBan0001469|CT3604|) *F *F anon-EST:ParkEST270 Sap-r (CG12070|FBan0012070|CT4748|) *F *F anon-EST:ParkEST360 CG7348|FBan0007348|CT22655| *F *F anon-EST:ParkEST398 CG7298|FBan0007298|CT22503| *F *F anon-EST:Posey10 CG6105|FBan0006105|CT19171| *F *F anon-EST:Posey100 CG11771|FBan0011771|CT33040| *F *F anon-EST:Posey103 CG6514|FBan0006514|CT20253| *F *F anon-EST:Posey104 CG5644|FBan0005644|CT16909| *F *F anon-EST:Posey105 CG12194|FBan0012194|CT10091| *F *F anon-EST:Posey109 CG13929|FBan0013929|CT33468| *F *F anon-EST:Posey113 CG10802|FBan0010802|CT10681| *F *F anon-EST:Posey114 CG13094|FBan0013094|CT32319| *F *F anon-EST:Posey116 CG2789|FBan0002789|CT9517| *F *F anon-EST:Posey12 CG7224|FBan0007224|CT22279| *F *F anon-EST:Posey120 CG10157|FBan0010157|CT28567| *F *F anon-EST:Posey121 Med (CG1775|FBan0001775|CT5334|) *F *F anon-EST:Posey125 CG1475|FBan0001475|CT2504| *F *F anon-EST:Posey127 CG15697|FBan0015697|CT35898| *F *F anon-EST:Posey132 CG4721|FBan0004721|CT15195| *F *F anon-EST:Posey135 CG9374|FBan0009374|CT26569| *F *F MERGE *F anon-EST:Posey137 CG8332|FBan0008332|CT24587| *F anon-EST:Posey185 CG8332|FBan0008332|CT24587| *F *F anon-EST:Posey14 scf (CG9148|FBan0009148|CT1311|) *F *F MERGE *F anon-EST:Posey140 CG8395|FBan0008395|CT18601| *F anon-EST:Posey187 CG8395|FBan0008395|CT18601| *F *F anon-EST:Posey141 CG4769|FBan0004769|CT15355| *F *F anon-EST:Posey143 CG11015|FBan0011015|CT30186| *F *F anon-EST:Posey148 EG:114D9.1 (CG11408|FBan0011408|CT31847|) *F *F anon-EST:Posey150 Cyp9f2 (CG11466|FBan0011466|) *F *F anon-EST:Posey151 MERGE with noe, no CG annotated *F *F anon-EST:Posey153 CG7630|FBan0007630|CT23291| *F *F anon-EST:Posey157 CG4111|FBan0004111|CT13646| *F *F anon-EST:Posey158 CG8415|FBan0008415|CT24703| *F *F anon-EST:Posey160 CG7646|FBan0007646|CT23353| *F *F anon-EST:Posey161 CG8369|FBan0008369|CT24651| *F *F anon-EST:Posey162 CG6549|FBan0006549|CT20403| *F *F anon-EST:Posey167 CG3560|FBan0003560|CT11966|CT13660| *F *F anon-EST:Posey169 CG9890|FBan0009890|CT27874| *F *F anon-EST:Posey170 CG2162|FBan0002162|CT7070| *F *F anon-EST:Posey173 CG9954|FBan0009954|CT28013| *F *F anon-EST:Posey177 CG7221|FBan0007221|CT22265| *F *F anon-EST:Posey179 Qm (CG17521|FBan0017521|CT38737|) *F *F anon-EST:Posey18 CG12775|FBan0012775|CT36295| *F *F anon-EST:Posey180 CG6617|FBan0006617|CT20578| *F *F anon-EST:Posey185 CG8332|FBan0008332|CT24587| MERGE *F *F anon-EST:Posey187 CG8395|FBan0008395|CT18601| MERGE *F *F anon-EST:Posey188 CG7283|FBan0007283|CT22467| *F *F anon-EST:Posey189 CG10186|FBan0010186|CT28647| *F aligned AE003663 and AF171833; EST predicts *F an additional exon at complement (<73055..73437) *F *F anon-EST:Posey2 CG7701|FBan0007701|CT23463| *F *F anon-EST:Posey20 CG16947|FBan0016947|CT37598| *F (aligned AF171768 to AE003613, alignment extends *F further than shown in our BLAST output) *F *F anon-EST:Posey203 miple (CG1221|FBan0001221|CT2033|) *F *F anon-EST:Posey205 CG9905|FBan0009905|CT27898| *F *F anon-EST:Posey213 vimar (CG3572|FBan0003572|CT12008|) *F *F anon-EST:Posey214 CG11752|FBan0011752|CT5038| *F *F anon-EST:Posey219 CG7361|FBan0007361|CT22681|CT31879| *F *F anon-EST:Posey224 CG1746|FBan0001746|CT5086| *F *F anon-EST:Posey227 CG9394|FBan0009394|CT26667| *F (extends CG at 3' end) *F *F anon-EST:Posey228 CG7630|FBan0007630|CT23291| MERGE *F *F anon-EST:Posey230 CG1552|FBan0001552|CT4006| *F (check intron-exon boundaries of CG) *F *F anon-EST:Posey231 CG17753|FBan0017753|CT11457| *F (aligned AF083312 to AE003830; alignment extends *F further than in our BLAST output) *F *F anon-EST:Posey234 CG6783|FBan0006783|CT21061| *F *F anon-EST:Posey235 BcDNA:GH07626 (CG3523|FBan0003523|CT11871|) *F *F anon-EST:Posey237 CG17515|FBan0017515|CT33382| *F (AF171847 aligned to AE003066, extends CG) *F *F anon-EST:Posey240 smt3 (CG4494|FBan0004494|CT14617|) *F *F anon-EST:Posey242 CG9282|FBan0009282|CT26439| *F *F anon-EST:Posey244 CG12840|FBan0012840|CT31972| *F *F anon-EST:Posey245 CG4169|FBan0004169|CT13760| *F *F anon-EST:Posey250 CG7239|FBan0007239|CT22327| *F *F anon-EST:Posey253 CG1837|FBan0001837|CT5608| *F *F anon-EST:Posey256 CG1545|FBan0001545|CT4000| *F *F anon-EST:Posey261 CG4561|FBan0004561|CT14730| *F *F anon-EST:Posey265 Transferrin (CG6186|FBan0006186|CT19250|) *F *F anon-EST:Posey266 CG3229|FBan0003229|CT10811| *F (differs from CG at 3' end but sequence matches scaffold *F look for alternate splice or correction of CG sequence) *F *F anon-EST:Posey267 CG9240|FBan0009240|CT26394| *F (extends CG at 3' end) *F *F anon-EST:Posey272 CG17737|FBan0017737|CT2632| *F *F anon-EST:Posey275 CG5537|FBan0005537|CT17516| *F *F anon-EST:Posey276 CG3203|FBan0003203|CT10544| *F *F anon-EST:Posey279 CG9473|FBan0009473|CT26832| *F *F anon-EST:Posey281 CG11943|FBan0011943|CT35760| *F *F anon-EST:Posey282 CG12014|FBan0012014|CT1651| *F *F anon-EST:Posey284 CG15494|FBan0015494|CT35596| *F *F anon-EST:Posey287 CG11388|FBan0011388|CT31798| *F *F anon-EST:Posey29 CG6673|FBan0006673|CT20732| *F *F anon-EST:Posey291 BM-40/SPARC (CG6378|FBan0006378|CT19876|) *F *F anon-EST:Posey293 RecQ5 (CG4879|FBan0004879|CT15495|) AF171784 aligned to RecQ *F *F anon-EST:Posey295 CG10423|FBan0010423|CT29278| *F *F anon-EST:Posey3 CG9686|FBan0009686|CT6802| *F (AF171762 aligned to AE003448) *F *F anon-EST:Posey31 CG8398|FBan0008398|CT24665| *F (AF171771 aligned to AE003562; extends last exon) *F *F anon-EST:Posey36 EG:115C2.12 (CG18451|FBan0018451|CT32691|) *F AF171773 aligned to AL031581 *F *F anon-EST:Posey39 CG2099|FBan0002099|CT6814| *F *F anon-EST:Posey4 eIF-1A (CG8053|FBan0008053|CT24166|) *F *F anon-EST:Posey40 CG4071|FBan0004071|CT13482| (EST extends CG4071) *F *F anon-EST:Posey42 CG5687|FBan0005687|CT8701| *F *F anon-EST:Posey43 BcDNA:GH07626 (CG3523|FBan0003523|CT11871|) *F *F anon-EST:Posey45 CG4692|FBan0004692|CT15135| *F *F anon-EST:Posey47 CG10320|FBan0010320|CT28984| *F merge with anon-EST:Posey84 *F *F anon-EST:Posey49 CG5972|FBan0005972|CT18757| *F *F anon-EST:Posey50 AP-50 (CG7057|FBan0007057|CT21823|) *F *F anon-EST:Posey54 CG11376|FBan0011376|CT31756| *F *F anon-EST:Posey62 CG3214|FBan0003214|CT10813| *F *F anon-EST:Posey63 CG8415|FBan0008415|CT24703| *F *F anon-EST:Posey66 CG5321|FBan0005321|CT16936| *F *F anon-EST:Posey73 RpL29 (CG10071|FBan0010071|CT28349|) (aligned AF083519 with U40226) *F *F anon-EST:Posey76 CG8309|FBan0008309|CT24557| *F *F anon-EST:Posey8 CG3792|FBan0003792|CT12669| *F *F anon-EST:Posey81 CG2176|FBan0002176|CT6332| *F *F anon-EST:Posey84 CG10320|FBan0010320|CT28984| AF171790 *F merge with anon-EST:Posey47 *F *F anon-EST:Posey85 CG15067|FBan0015067|CT34938| *F *F anon-EST:Posey87 CG5903|FBan0005903|CT18297| *F *F anon-EST:Posey91 CG8844|FBan0008844|CT9259| *F *F BEST:CK00325 Sur (CG5772|FBan0005772|CT18104|) *F *F BEST:CK00230 KdelR (CG5183|FBan0005183|CT16555|) *F *F BEST:CK00246 CG13920|FBan0013920|CT33459| *F *F BEST:CK00459 CG8083|FBan0008083|CT8107| *F *F BEST:CK00539 CG5912|FBan0005912|CT15575| *F *F BEST:CK01110 CG16982|FBan0016982|CT32695| *F *F BEST:CK01140 CG2165|FBan0002165|CT6738| *F *F BEST:CK01209 CG9085|FBan0009085|CT26050| *F *F BEST:CK01227 CG14709|FBan0014709|CT34500| *F *F BEST:CK01296 CG5885|FBan0005885|CT18469| *F *F BEST:CK01510 CG2768|FBan0002768|CT9403| *F *F BEST:CK01577 CG12789|FBan0012789|CT37157| *F *F BEST:CK02137 CG11163|FBan0011163|CT31188| *F *F BEST:CK02213 CG3814|FBan0003814|CT12775| *F *F BEST:CK02248 Tunen (CG8805|FBan0008805|CT3034|) *F *F BEST:CK02288 CG2165|FBan0002165|CT6738| *F *F BEST:CK02318 CG7144|FBan0007144|CT22073| *F *F BEST:CK02467 CG11592|FBan0011592|CT33137| *F *F BEST:LD04728 CG11546|FBan0011546|CT36453| *F *F BEST:LD04967 l(2)k05815(CG2207|FBan0002207|CT7302|) *F *F BEST:LD04971 CG11045|FBan0011045|CT28033| *F *F BEST:LD06340 yoyo transposon *F *F BEST:LD07107 AE003529.2:57569..58140 *F *F BEST:LD07122 CG9418|FBan0009418|CT26718| *F *F BEST:LD08487 CG12253|FBan0012253|CT15065| *F *F BEST:LD09360 nonA-l (CG10328|FBan0010328|CT28998|) (AA390491 aligned to nonA-l) *F *F BEST:LD12308 The 5' and 3' ends of this EST AA438512 align with CG3171 Trehalose *F BUT the middle has many mismatches. suspect. *F *F BEST:LD12957 CG10528|FBan0010528|CT37301| *F *F BEST:LD13681 CG16833|FBan0016833|CT15001| *F *F BEST:LD14744 CG7832|FBan0007832|CT23772| *F *F BEST:LD14959 CG8677|FBan0008677|CT5294| *F *F BEST:LD23852 CG3558|FBan0003558|CT11970| *F *F BEST:LD27171 BG:DS09218.3 (CG4455|FBan0004455|CT14482|) *F *F BEST:LD29214 CG8290|FBan0008290|CT24533| *F *F BEST:LD29743 CG4747|FBan0004747|CT15275| (mismatches in 5' 113 bp of EST, then OK) *F *F BEST:LD29847 CG9539|FBan0009539|CT26986| *F *F BEST:LD30049 CG5704|FBan0005704|CT2731| *F *F BEST:LD32772 Hsc70Cb (CG6603|FBan0006603|CT39144|) *F *F BEST:LD33989 CG5981|FBan0005981|CT18793| *F (this EST listed as evidence) *F *F Please add: *F BEST:LD13441 (Genbank acc. AA439017) CG14478|FBgn0034222|CT34189| *F ___________________________________________________________________ *F nc6 *F *F abo CG6093|FBgn0032323|CT19161| *F based on sequence sent by Dr. Pimpinelli (FBrf0129572). *F *F anon-18DEa CG14224|FBan0014224|CT33839| *F *F anon-18DEb CG14226|FBan0014226|CT33841| *F *F anon-18DEd CG14233|FBan0014233|CT33849| *F *F anon-18DEe CG14230|FBan0014230|CT33846| *F *F anon-61C this is 'interband DNA', shouldn't be assigned to a CG gene *F *F anon-85Da CG16750|FBan0016750|CT32111| (alignment extends CG at 5' end) *F *F anon-85Db this is DNA AE003682:113634..114395, 102059.. 103900,103923..104221 *F 1st segment flipped with respect to rest *F *F anon-a 1-173 of 250 matches CG-less sequence on AE003483 *F no CT immunoglobin switch region-like. *F *F anon-f CG1077|FBan0001077|CT1429| *F *F anon-FF CG6156|FBan0006156|CT19304|CT19330 *F *F anon-ft1 region of chromosomal breakpoint, DO NOT assign CG's, *F but can note that it aligns with the region containing *F CG3086 and CG15770 (AE003435:135304..137515) *F *F anon-Liu CG17270|FBan0017270|CT35903| *F *F anon-OV1 phr (CG11205|FBan0011205|CT31302|) *F (accession D26136 identical to accession S73530 listed under *F phr) *F *F anon-Pen16 clone may be chimeric *F [CG12136|FBan0012136|CT7794|] *F (1st 65 bp don't match CG12136, *F 1st 65 bp matches CG17251|FBan0017251|CT35403|) *F *F BcDNA:LD22118 CG4411|FBan0004411|CT14362| *F *F BcDNA:LP06355 Cyp304a1 (CG7241|FBan0007241|CT22337|) *F *F chitin-synthase CG7464|FBan0007464|CT22967| *F *F CycK CG15218|FBan0015218|CT35154 *F *F Dtf-1 TransFac entry only, not characterized molecularly *F (binds Antp promoter) *F *F EB1 CG3265|FBan0003265|CT10989| *F *F Ec3 AE003809:237082..237118 matches 1-37 *F AE003799:38783..38907 matches 38-160 *F *F EG:BACR7A4.7 CG11639|FBan0011639|CT34386| *F (following transcript and translation provided by Takis Benos, *F not in EMBL/GB entry AL109630: *F *F >BACR7A4.7 drosophila melanogaster (fruit fly). transcription initiation factor iia gamma chain (tfiia p14 subunit) (tfiia-14) (dtfiia-s) (tfiia-gamma). *F atgaactatcaacattacagggccacaacgctgggcagaacgctccagga *F cactttggatgagatgatggagaggggcgatataaccaagaagattgcta *F atttggttttactaagatatgacaagagcatttcaacagctctcaaggat *F catggcacgagcaatatgtcctttacggccgaaagactggaaacctttag *F atgctgcgacaatgtgtggactctgatcctaaaggacgcggagttccgcg *F aggatcagcattccttgaaggtcgatgtggtcaaaattgtggcctgtctt *F ggaactgacaatggaaatgaataa *F *F *F *F >BACR7A4.7 drosophila melanogaster (fruit fly). transcription initiation factor iia gamma chain (tfiia p14 subunit) (tfiia-14) (dtfiia-s) (tfiia-gamma). *F MNYQHYRATTLGRTLQDTLDEMMERGDITKKIANLVLLRYDKSISTALKD *F HGTSNMSFTAERLETFRCCDNVWTLILKDAEFREDQHSLKVDVVKIVACL *F GTDNGNE* *F *F Eip63F-2 matches CG-less sequence on AE003479 *F ('Coding sequence unknown, according to accession U27300') *F putative partial translation given in paper [FBrf0083007], *F no match to it among predicted proteins. *F *F fok - CG10746|FBan0010746|CT30111| *F possibly untranslated *F CT translation not available *F *F Gebf-I TransFac entry only, not characterized molecularly *F *F *F Hemagglutinin no matches in AE sequences, only other match is *F nt 74-219 matches 1-146 of Influenza A virus *F *F hrt P insertion site flank *F matches CG-less sequence at AE003677:143024..143516 *F *F LB27 MERGE with yoyo; matches yoyo retrotransposon LTR *F *F lectin-24Da CG-less (U. Theopold, personal comm. curated by Camb.) *F lectin-24Fa CG-less (U. Theopold, personal comm. curated by Camb.) *F lectin-24Fb CG-less (U. Theopold, personal comm. curated by Camb.) *F *F MS:DS01001 this is a collection of many accessions characterizing *F repeat regions *F *F Myc does not align with any other Drosophila DNA in GenBank, *F BLAST with nr (non-redundant) and dbEST databases. *F some alignment with unfiltered BLAST: possible repeat *F element? *F *F Pglym87 [CG17645|FBan0017645|CT28399] *F possible PSEUDOGENE as per FB gene report *F (Currie and Sullivan, 1994). *F *F Pk17E CG7001|FBan0007001|CT21577| *F *F Pk36A grp (CG17161|FBan0017161|CT14720|) *F *F Pk53C Cdk4/6 (CG5072|FBan0005072|CT15896|) *F *F Pk91C CG7719|FBan0007719|CT23435| *F *F RpL12 >CG3195|FBgn0034968| *F *F (PROBLEM: RpL12 has been mapped to 62E, CG3195 to 60B2-3) *F *F CG3195 and CG10485 are transcribed off opposite strands, *F RpL12 doesn't have a CDS annotated, check with other organisms: *F *F >CG3195|FBgn0034968 *F MPPKFDPTEVKLVYLRCVGGEVGATSSLAPKIGPLGLSPKKIGDDIAKAT *F SDWKGLKITVCLTIQNRQAAISVVPSAASLIIKALKEPPRDRKKQKNIKH *F SGNIGFEDILAIARVMRPRSMARELKGTCKEVLGTAQSVGCTVDGKHPHD *F VIDELNEGSIEVPAE *F *F >CG10485|FBgn0034969 *F MDLGLITRAMARMSSKPMFPLCLTSRIAGQYSLFFCFLRSRGGSFRALMI *F SEAAEGTTEMAACRFWMVRQTVIFRPFQSEVALAMSSPIFLGDYRETLLL *F ARIAQVNKPICMLTRPRGPIFGAREDVAPTSPPTQRRYTVIWVQKFVIED *F NPLL *F *F Mouse RpL12: *F >gi|398048|gb|AAA40066.1| ribosomal protein L12 *F MPPKFDPNEVKVVYLRCTGGEVGATSALAPKIGPLGLSPKKVGDDIAKAT *F GDWKGLRITVKLTIQNRQAQIEVVPSASGLIIKALKEPPRDRKKQKNIKH *F SGNITFDEIVNIARQMRHRSLARELSGTIKEILGTAQSVGCNVDGRHPHD *F IIDDINSGAVECPAS *F *F *F RpL31 CG1821|FBan0001821|CT5526| *F *F sw59 lola (CG12052|FBan0012052|) *F *F ted only accession listed is a short putatively intronic fragment *F of 70 bp (aligns to AE003672:152669..152738) *F *F *F _____________________________________________________________________ *F The correspondence of the following Ugt genes to CG genes were *F verified by Dr. Uli Theopold: *F *F Ugt58Fg CG4414 *F Ugt36Ba CG13270 *F Ugt36Bb CG13271 *F Ugt36Bc CG17932 *F Ugt37b1 CG9481 *F Ugt37c1 CG8652 (*CG8652 is shorter than Ugt37c1 at the N-terminus.) *F Ugt86Da CG18578 *F Ugt86Db CG6649 (probably a polymorphic variant of Ugt35b, see FBrf0110927) *F Ugt86Dc CG4739 *F Ugt86Dd CG6633 *F Ugt86De CG6653 *F Ugt86Df CG6644 (probably a polymorphic variant of Ugt35a, see FBrf0110927) *F Ugt86Dg CG17200 *F Ugt86Dh CG4772 *F Ugt86Di CG6658 *F Ugt86Dj CG15902 *F _____________________________________________________________________ *F *F Vha100-1 BcDNA:LD21248 (CG1709) *F Vha100-2 BcDNA:LD21735 (CG18617) *F Vha100-3 author queried Aug.25 (whole contig accession is given *F in paper; coordinates unknown) *F *F Vha16-2 author queried Aug.25 ' ' *F Vha16-3 author queried Aug.25 ' ' *F Vha16-4 author queried Aug.25 ' ' *F *F VhaAC39 CG2934|FBan0002934|CT9953| *F VhaM9.7-1 CG11589|FBan0011589|CT36548 *F VhaM9.7-2 CG7625|FBan0007625|CT23265 *F VhaPPA1-1 CG7007|FBan0007007|CT21672 *F VhaPPA1-2 author queried Aug.25 *F *F *F yip1 CG18497|FBan0018497|CT42170| *F *F yip4 matches CGless sequence on AE003519:250616..251056 *F *F Addenda to nc6 list *F >From FBrf0108062 *F *F gene accession Celera gene *F BEST:LD25345 AA941286 CG17322 *F *F BEST:GH06505 AI107184 CG17324 *F *F BEST:GH09393 AI109977 CG4302 *F *F BEST:GM04645 AA695862 CG18578 (matches Ugt86Da) *F *F AntP450 = Cyp6w1 (CG8345) *F *F AntP450 dentified with: GH06928 accession AI113367 *F which aligns perfectly with CG8345 corresponding to Cyp6w1 *F (AI113367 is listed among the evidence for Cyp6w1) *F *F Ugt37a1 CG11012 *F (DS51087 complement 6570..68193) *F *F EfSec CG9841 *F (Tujebajeva et al 2000 EMBO reports 1(2)158-163) *F *F robl22E CG10838|FBan0010838|CT30347| *F *F robl37BC CG15171|FBan0015171|CT35080| *F *F robl62A CG1014|FBgn0035222|CT1028| *F *F BEST:CK02567 CG5661|FBan0005661|CT17880| *F *F BEST:CK02623 CG15438|FBan0015438|CT35502| *F *F BEST:CK02656 CG3164|FBan0003164|CT10462| *F *F BEST:GM02209 CG3696|FBan0003696|CT12335| *F *F BEST:GM02553 CG6530|FBgn0034220|CT20339| accession AA695295 *F *F BEST:GM10514 CG10161|FBan0010161|CT28571| *F *F BEST:HL03644 CG5707|FBan0005707|CT2751| *F *F BEST:HL04053 CG1079|FBan0001079|CT1463| (extends CG at 5' end) *F *F BEST:LD02456 matches CG-less at AE003761:145689..145157 *F *F BEST:LD03274 lola (CG12052|FBan0012052|CT40980|) *F *F BEST:LD03829 homer (CG11324|FBan0011324|CT31607|) *F *F BEST:LD04728 CG11546|FBan0011546|CT36453| *F *F BEST:LD21971 CG11518 (please add accession AA817007) *F *F CaBP1 BG:DS09218.4 (CG5809|FBan0005809|CT18216|) *F (aa seq from FBrf0104763, matched by BLASTP) *F *F Las CG5231 *F by BLASTN alignment with AA820940 and AA201873 [FBrf0111379] *F *F RpL37a CG5827 *F sequence in [FBrf0111872], not submitted to GB, adjacent to qtc *F *F att CG4241|FBan0004241|CT13930| *F sequence in [FBrf0089733] but not in GenBank *F Celera translation is further 5' than the one in [FBrf0089733] *F *F primo-1 and primo-2 correspond to CG9599 (based on predicted peptide *F in FBrf0125124).CG9599 has to be split and intron-exon structure corrected. *F *F qtc CG14039|FBan0014039|CT33598| *F sequence in FBrf0127087 but not in GenBank *F *F Gyk CG18374 (curator inference) *F According to FBrf0103348 figure 3 NitFhit is in the *F intron of Gyk (opp.strand). BLASTP of predicted translation *F of CG18374 gives many hits to glycerol kinases (top 5 below) *F gb|AAF47346.1| (AE003467) CG18374 gene product [Drosophila ... 1038 0.0 *F ref|NP_034424.1| glucokinase activity, related sequence 2 >... 549 e-155 *F ref|NP_032220.1| glycerol kinase >gi|2493484|sp|Q64516|GLPK... 543 e-153 *F emb|CAB54858.1| (AJ252550) glycerol kinase [Homo sapiens] 542 e-153 *F sp|Q63060|GLPK_RAT GLYCEROL KINASE (ATP:GLYCEROL 3-PHOSPHOT... 542 e-153 *F *F Lip2 CG17116 (curator inference) *F [FBrf0102171] states Lip2 is about 1 kb away from Lip1 *F Lip2+ anon-32Aa- Lip2+ Lip1+ *F [FBrf0102171] states that Lip2 may be nonfunctional *F GHSQV is GHSQA in Celera sequence *F *F anon-32Aa CG6415 (curator inference) *F [FBrf0102171] states that there is an aminomethyltransferase *F subunit in the putative 3rd intron of Lip2 (FB symbol anon-32Aa). *F *F ND23 CG3944 (curator inference) *F (ND23 is a NADH dehydrogenase adjacent to spn-E *F order ND23- spn-E+) *F *F Sti1 Hop (CG2720) curator inference *F Hop is listed in the accession AF056198 *F as being a 'yeast Sti1p homolog' *F Detailed molecular map given in Current Biol 9:1019 [FBrf0111517] *F smo+ U2af38- Sti1+ Pi3K21B+ Plc21C+ *F CG2720 is between U2af38 and Pi3K21B. *F *F pkaap BG:DS02740.4 (CG4132) curator inference *F CG4132 has homology to protein kinase A anchoring proteins *F adjacent to crp and on opposite strand *F [FBrf0110073] order twe- crp- pkaap+ (additional gene predicted betw. *F twe and crp in Celera and BDGP sequences) *F *F snRNP69D CG10753 curator inference *F gene order from [FBrf0086377] snRNP69D- Ptp69D+ Klc- *F stated to be 1 kb upstream from and transcribed in opposite direction *F from Ptp69D. CG10753 shows homology to small nuclear ribonucleoproteins # *U FBrf0132094 *a Blair *b S. *t 2000.12.7 *T personal communication to FlyBase *u FlyBase error report on Wed Dec 6 17:32:42 2000. *F Delivery-date: Wed, 6 Dec 2000 23:35:19 \+0000 *F Date: Wed, 06 Dec 2000 17:32:42 \-0600 *F To: Chihiro Yamada *F From: 'Seth S. Blair' *F Subject: Re: FlyBase Query (cy901a) *F CG9841 contains obvious EFTU-like sequence, but we did worry that portions *F of the two CG's should perhaps be merged. We got conflicting evidence on *F this. As we said in the paper, running the region through the Sanger *F Genefinder (fgenesh) predicted a single gene instead of the two you listed *F (if you want I can send you the predicted exons). And while the portion of *F that prediction that corresponds to the 'second' gene (CG10795) is not *F obviously similar to EFTU, it does have some similarity to GTP-binding *F proteins, and EFTUs bind GTP. *F On the other hand, other gene predictors split this up into two different *F genes. And the LD27358 EST sequence corresponds only to CG10795, and *F contains what looks like 5' UTR sequence sitting between the two genes. So *F either there really are two genes, or LD27358 contains some unspliced *F intron sequence. There don't seem to be any other ESTs for this gene. *F Beyond that, we did not do anything to figure out which was right. *F Sorry, wish I could be of more help. Certainly not strong enough evidence *F to say your prediction is in error. *F Also, just curious about one thing. Do the cDNA Fasta's in Gadfly *F correspond only to the ORF? The release 1 CG10795 starts with an ATG, but *F there's a stop shortly after it, and the translation Fasta starts much later. *F Seth *F Seth S. Blair *F Department of Zoology *F University of Wisconsin *F 250 N. Mills St. *F Madison, WI 53706 *F (608) 262-1345, 2-1426, FAX 2-9083 *F ssblair@facstaff.wisc.edu # *U FBrf0132095 *a Klaembt *b C. *t 2000.11.3 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Oct 19 17:57:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 19 Oct 2000 17:57:43 \+0100 *F To: klaembt@mail.uni-muenster.de *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 19 Oct 2000 17:57:45 \+0100 *F Content-Length: 2984 *F Dear Dr. Klaembt, *F I wrote to A. Michelson about a construct he used in a paper (as I *F think it is a new construct that FlyBase doesn't have a record of yet) *F that he obtained from you and he suggest I write to you to confirm *F details of this construct. *F The paper was: *F Halfon et al, 2000, Cell 103(1): 63--74 *F and the construct is an activated UAS-pnt construct, which was called *F 'UAS-pntP2Act' or 'UAS-pntP2VP16' ( = superscript) in the paper. *F here is what I asked A. Michelson about the construct: *F > 2. UAS-pntAct ( = superscript) *F > *F > This construct is called 'UAS-pntP2VP16' in the Materials and Methods. *F > *F > I cannot find any record of an activated UAS-pnt construct in FlyBase, *F > so I think it must be new (if it has been published previously, please *F > could you tell me the reference). *F > *F > If it is new, I would be grateful if you could provide some details *F > about the construct (which I would curate as a personal communication *F > from you to FlyBase) so that users of FlyBase can see what the *F > construct consists of. *F > *F > Could you tell me: *F > *F > a. who made the construct (this helps in naming it) ? *F > b. is it the P2 isoform of pnt (as P2 was in the symbol) ? *F > c. what is the nature of the activating mutation \- presumably this is *F > what the VP16 stands for ? *F > d. what vector was used to make the construct, e.g. pUAST, pCaSpeR *F > (this helps in working out the marker allele in the construct) ? *F here is what he replied: *F > 2. UAS-PntP2VP16 = UAS-PntP2Act was constructed and transgenic lines *F > were generated by Christian Klambt; to my knowledge, the details of its *F > contruction have not been published; however, my understanding is that it *F > contains the strong transcriptional activation domain of the herpes simplex *F > virus VP16 protein fused to a part of the PntP2 coding region; you would *F > have to contact Christian if you really need to know the precise details of *F > the construct; in any case, you should verify this question with him since *F > he is the actual source of the materials *F > *F > 3. I assume that pUAST must have been the vector used to make *F > UAS-PntP2VP16, but again Christian would be the one to confirm that *F > question. *F I would be grateful if you could confirm these details, specifically *F whether it is the 'P2' isoform of pnt that is used, whether 'VP16' does *F mean the transcriptional activation domain of herpes VP16 protein, and *F whether the vector used was pUAST. *F The information about this construct would be recorded in FlyBase as a *F personal communication from you to FlyBase, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From klaembt@uni-muenster.de Thu Nov 02 13:55:03 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 2 Nov 2000 13:55:03 \+0000 *F X-Sender: klaembt@pop.uni-muenster.de *F Mime-Version: 1.0 *F Content-Transfer-Encoding: quoted-printable *F Date: Thu, 2 Nov 2000 14:54:08 \+0100 *F To: Gillian Millburn (Genetics) *F From: Christian Klaembt *F Subject: Re: FlyBase query *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Length: 2407 *F >Dear Dr. Klaembt, *F > *F >I wrote to A. Michelson about a construct he used in a paper (as I *F >think it is a new construct that FlyBase doesn't have a record of yet) *F >that he obtained from you and he suggest I write to you to confirm *F >details of this construct. *F > *F >The paper was: *F > *F >Halfon et al, 2000, Cell 103(1): 63--74 *F > *F >and the construct is an activated UAS-pnt construct, which was called *F >'UAS-pntP2Act' or 'UAS-pntP2VP16' ( = superscript) in the paper. *F > *F >here is what I asked A. Michelson about the construct: *F > *F >> 2. UAS-pntAct ( = superscript) *F >> *F >> This construct is called 'UAS-pntP2VP16' in the Materials and Methods. *F >> *F >> I cannot find any record of an activated UAS-pnt construct in FlyBase, *F >> so I think it must be new (if it has been published previously, please *F >> could you tell me the reference). *F >> *F >> If it is new, I would be grateful if you could provide some details *F >> about the construct (which I would curate as a personal communication *F >> from you to FlyBase) so that users of FlyBase can see what the *F >> construct consists of. *F >> *F >> Could you tell me: *F >> *F >> a. who made the construct (this helps in naming it) ? *F the construct was made by Klaus Dücker from the Hafen lab in Zürich, the *F transgenic flies were generated in my lab- *F >> b. is it the P2 isoform of pnt (as P2 was in the symbol) ? *F it is the pointed P2 isoform *F >> c. what is the nature of the activating mutation \- presumably this is *F >> what the VP16 stands for ? *F the activation domain is from VP16, it relaces the pointed domain including *F the MAPkinase phosphorylation site *F >> d. what vector was used to make the construct, e.g. pUAST, pCaSpeR *F the construct was inserted in pUAST and injected in a w1118 background. *F best wishes christian klaembt *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F Dr. Christian Klämbt *F Institut fuer Neurobiologie *F Universität Münster *F Badestrasse 9 *F D-48149 Münster Germany *F Phone xx49 251 83 2 1122 *F FAX xx49 251 83 2 4686 *F email klaembt@uni-muenster.de # *U FBrf0132113 *a Whitfield *b E. *t 2000.10.31 *T personal communication to FlyBase *u FlyBase error report on Tue Oct 31 16:26:11 2000. *F From eleanor@ebi.ac.uk Tue Oct 31 16:35:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 31 Oct 2000 16:35:16 \+0000 *F Date: Tue, 31 Oct 2000 16:26:11 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: FlyBase error report for CGs on Tue Oct 31 2000 *F Content-Transfer-Encoding: 7bit *F Error reports from Eleanor Whitfield (eleanor@ebi.ac.uk) *F 1) *F Gene or accession: CG18740 *F Gene annotation error *F Genes CG18740 and mor should be merged. *F Comments: In the new release the updated translation in protein_ID AE003711; *F AAF55259 is wrongly assigned a new CG symbol, CG18740. In release 1 the *F gene was stated to be mor (moira). It still is moira with a better *F (correct!) translation. *F thanks *F 2) *F Gene or accession: CG18572 *F Gene annotation error *F Genes CG18572 and r should be merged. *F Comments: In the new release the updated translation in protein_ID *F AE003503; AAF48639 is wrongly assigned a new CG symbol, CG18572. In release *F 1 the gene was stated to be r (rudimentary). It still is rudimentary with a *F better, but still incorrect, translation. *F thanks *F 3) *F Gene or accession: CG1706 *F Gene annotation error *F Genes CG1706 and CG11166 should be merged. . *F Comments: CG1706 no longer exists in Celera release 2. Translation for *F AE003841; AAF59266 was updated now is known to be a splice variant of *F CG11166. Will CG11166 be the valid symbol as CG1706 has been dropped in *F release 2 (ie the numerically first CG will not be the valid symbol)? *F thanks *F 4) *F Gene or accession: CG14767 *F Gene annotation error *F Genes CG8575 and CG14767 should be merged. . *F Comments: CG8575 no longer exists in Celera release 2. Translation for *F AE003836; AAF59050 was updated now is known to be a splice variant of *F CG14767. Will CG14767 be the valid symbol as CG8575 has been dropped in *F release 2 (ie the numerically first CG will not be the valid symbol)? *F thanks *F 5) *F Gene or accession: CG18438 *F Gene annotation error *F Genes CG18438 and brm should be merged. *F Comments: In the new release the translation in protein_ID AE003529; *F AAF49557 *F remains unchanged but the annotation is changed so that the correct gene *F symbol is annotated \- brm. Please merge CG18438 and brm. *F thanks *F 6) *F Gene or accession: CG10517 *F Gene annotation error *F Genes CG10167 and CG10517 should be merged. *F Comments: In the new release the translation and annotation in protein_ID *F AE003593; AAF51686 has been changed, and the annotation of AE003593; *F AAF51685. Please merge CG10167 and CG10517, they are splice variants of one *F gene. Will CG10517 be the valid symbol as CG10167 has been dropped in *F release 2 (ie the numerically first CG will not be the valid symbol)? *F thanks *F 7) *F Gene or accession: CG1540 *F Gene annotation error *F Genes CG1540 and CG18436 should be merged. *F Comments: In the new release the translation and annotation in protein_ID *F AE003765; AAF56794 has been changed, and the translation of AE003765; *F AAF56795. They are now annotated as being 2 genes when in fact they are *F splice variants of the same gene. Here I am guessing the numerically first *F symbol CG1540 will be valid as both symbols have the same 'status'. *F thanks *F Nellie # *U FBrf0132114 *a Zhu *b W. *t 2000.10.31 *T personal communication to FlyBase *u FlyBase error report for CG8276 on Tue Oct 31 08:35:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Oct 31 16:36:04 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 31 Oct 2000 16:36:04 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Oct 2000 08:35:46 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: zhu00006@mc.duke.edu *F Subject: FlyBase error report for CG8276 on Tue Oct 31 08:35:46 2000 *F Content-Length: 1582 *F Error report from Wencheng Zhu (zhu00006@mc.duke.edu) *F Gene or accession: CG8276 *F cDNA or EST error *F Comments: The full length cDNA for bin3 has been obtained using 5' RACE. The *F data has not deposited into Genbank. There are two isoforms for bin3 that is *F very likely to result from two promoter activity and alternative splicing as *F well. Thus bin3 has two different 5' UTRs. The sequence in Genbank is just *F the common sequence between these two different isoforms. *F Distal 5’ UTR *F 1 TGAG TTGTAAGTGT TTTCTTGCAG GAGACTGACG CAATTATTAG 44 *F 45 AGCTCCGCGA ACGGAATCAT ATTTTACGGT TTTTTTTTTT TTTATCAAGA GTGTGGCGAA *F TTTAATCGGC AACAACGAAA 124 *F 125 CGAAAAGTGA AAACAAAGTT CAAGTCGCCA CTGAGCAATC GTGGGGCTTT CTGGGCGACT *F CTTGCAGGGT ACATCTCAAC 224 *F Proximal 5’ UTR *F 1 AAACCCGCGA CGCATTCGGA CTTCAAGCAA GAGTCCGCTT TGCCGAGATA TAAAATTAAT *F AACGAGATCG AGTACCAGCT 80 *F 81 GCACACAGTG GAAATGAGAA AAGACCGACG GCAAAACAAT AGAACACACC CGATTAGTCG *F TGCGTAACCG ATTGACTAAA 160 *F 161 GCACGGGGCA GAGTCGATAG AAAAAATATA CAGTTTTAAA GCGCTTAATT AGGTGTTTTC *F TAACGTTGGT ACATCTCAAC 240 *F 241 GGAGTGGATA ACGAGAAGAG TGAAGGGAGG AGAACCATTG GCAAGAACAT ACTCACCAAA *F ATGGATAATT TCGATAAAAT 320 *F 321 ATTTAACAGT GAAAGTGAAA ACGGTTGAAG TTTTAAAATA AAAAGAAATA ACTCGTACGC *F CAAAGGATGC AATATTAAGT 400 *F For details, see Wencheng Zhu Ph.D dissertation, Appendix, SUNY-Albany. *F Mentor: Dr. Steven D. Hanes. *F If you have any more questions, please contact me. *F Browser: Mozilla/4.0 (compatible; MSIE 5.5; Windows NT 4.0) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132115 *a Whitfield *b E. *t 2000.11.1 *T personal communication to FlyBase *u FlyBase error report for CG2916 on Wed Nov 1 01:51:47 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Nov 01 09:51:54 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 1 Nov 2000 09:51:54 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 1 Nov 2000 01:51:47 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2916 on Wed Nov 1 01:51:47 2000 *F Content-Length: 401 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2916 *F Gene annotation error *F Genes CG2916 and Sep5 should be merged. *F Comments: CG2916 ad Sep5 should be merged as they are at the same location and *F have 100% *F identical protein translation: *F AE003839; AAG22304 *F AF167578; AAD49962 *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132116 *a Ruvinsky *b I. *t 2000.11.2 *T personal communication to FlyBase *u FlyBase error report on Thu Nov 2 13:49:32 2000. *F From bdgp-admin@fruitfly.bdgp.berkeley.edu Thu Nov 02 18:50:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 2 Nov 2000 18:50:50 \+0000 *F From: 'Ruvinsky, Ilya' *F To: ''bdgp@fruitfly.org'' *F Date: Thu, 2 Nov 2000 13:49:32 \-0500 *F MIME-Version: 1.0 *F X-Mailer: Internet Mail Service (5.5.2580.0) *F Subject: BDGP nompC annotation *F Sender: bdgp-admin@fruitfly.bdgp.berkeley.edu *F X-BeenThere: bdgp@fruitfly.BDGP.Berkeley.EDU *F X-Mailman-Version: 2.0beta6 *F List-Help: *F List-Post: *F List-Subscribe: , *F *F List-Id: BDGP mailing list *F List-Unsubscribe: , *F *F I've noticed that nompC is incorrectly annotated in BDGP. Compare to Walker et *F al. Science (2000) 287:2229-2234. There's an apparent frameshift in the database *F version. *F Ilya Ruvinsky. *F _______________________________________________ *F BDGP mailing list *F BDGP@fruitfly.BDGP.Berkeley.EDU *F http://fruitfly.BDGP.Berkeley.EDU/mailman/listinfo/bdgp # *U FBrf0132117 *a Whitfield *b E. *t 2000.11.3 *T personal communication to FlyBase *u FlyBase error report for CG17077 on Fri Nov 3 05:43:49 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Nov 03 13:44:07 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 3 Nov 2000 13:44:07 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 3 Nov 2000 05:43:50 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17077 on Fri Nov 3 05:43:49 2000 *F Content-Length: 566 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17077 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG17077. *F Comments: pnt is missing a splice variant. *F Klaembt, Development 117:163-176(1993) have identified two isoforms. *F Celera have translated isoform P2 (AE003742; AAF56125), but P1 is missing. *F Sequence of P1 has an alternative N-terminal exon (X69166; CAA48916) that is *F available in the DNA. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132118 *a Sisson *b J.C. *t 2000.11.4 *T personal communication to FlyBase *u FlyBase error report for CG6450 on Fri Nov 3 17:54:20 2000. *F From FlyBase-error@hedgehog.lbl.gov Sat Nov 04 01:54:19 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 4 Nov 2000 01:54:19 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 3 Nov 2000 17:54:20 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sisson@darwin.ucsc.edu *F Subject: FlyBase error report for CG6450 on Fri Nov 3 17:54:20 2000 *F Content-Length: 946 *F Error report from John C. Sisson (sisson@darwin.ucsc.edu) *F Gene or accession: CG6450 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG6450. *F Comments: In Dr. Bill Sullivan's lab I have identified the product of CG6450 *F as the Golgi body-associated protein Lava Lamp (LVA). The manuscript *F characterizing LVA will appear in the Nov. 13th issue of The JCB. We believe *F LVA functions on Golgi membrane to facilitate membrane trafficking and *F possibly interactions with microtubules. I can provide additional functional *F information upon request. However, I would like to report that we do not have *F evidence from our mass spectrometry analysis of LVA protein or from LVA cDNA *F sequence that the fifth predicted exon exists. Instead there appears to be a *F single small intron between the two large exons. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132119 *a Selby *b T.L. *t 2000.11.1 *T personal communication to FlyBase *u FlyBase error report on Wed Nov 1 11:11:50 2000. *F From bdgp-admin@fruitfly.bdgp.berkeley.edu Wed Nov 01 19:11:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 1 Nov 2000 19:11:31 \+0000 *F From: 'Thomas L. Selby' *F To: *F Date: Wed, 1 Nov 2000 11:11:50 \-0800 *F MIME-Version: 1.0 *F Content-Type: multipart/alternative; *F boundary='----=_NextPart_000_0087_01C043F4.883C2C60' *F X-Priority: 3 *F X-MSMail-Priority: Normal *F X-Mailer: Microsoft Outlook Express 5.50.4133.2400 *F X-MimeOLE: Produced By Microsoft MimeOLE V5.50.4133.2400 *F Subject: BDGP unknown vs psuedo genes *F Sender: bdgp-admin@fruitfly.bdgp.berkeley.edu *F X-BeenThere: bdgp@fruitfly.BDGP.Berkeley.EDU *F X-Mailman-Version: 2.0beta6 *F List-Help: *F List-Post: *F List-Subscribe: , *F *F List-Id: BDGP mailing list *F List-Unsubscribe: , *F *F Content-Length: 8105 *F Dear Drosophila database masters: *F I have a few general questions regarding what is being generated through the *F database and if there is a 'better' means of assessing some of the results. *F I am a protein chemist/structural biologist working on three drosophila *F proteins. The first protein is Roundabout-1 (Robo-1). This protein has been *F well characterized and demonstrates the common motifs found in cell adhesion *F proteins, and has thus been characterized as a cell adhesion protein in your *F database. This characterization is apparently wrong however because the Ig *F domains in robo are known to bind the slit protein and have no impact on cell *F adhesion. This leads to my first question about how experimental data is *F being incorporated into the databases that we currently have access to. I do *F understand that the task is difficult given the large number of sequences to *F be used. I feel some of this could be solved by integration with databases *F such as provided at the interactive fly: This would provide some experimental *F links and possibly help to identify homologous proteins. *F http://sdb.bio.purdue.edu/fly/aimain/1aahome.htm Is there some work in *F progress to link experimental data to the sequences? How or when can this be *F accessed? *F My second question is a bit more difficult to answer, but please provide any *F assistance that you think will be useful. As for the robo proteins, there are *F three members of the family robo-1,-2, and-3. Robo-1 has been characterized, *F but the other two have not. When the database is searched, robo-2 and robo-3 *F are found, but the identified cDNAs only include the extracellular portions of *F the proteins-which are composed of homologous Ig domains. The cytoplasmic *F tails (about 450 AA) are not found in the same entry, but are listed under the *F genomic scaffolds dumped into NCBI by Celera. Having worked on these domains, *F I know they are important for the function of the intact protein. The main *F problem (I believe) in identifying the protein is the absence of any *F homologous domains, which makes the translated cDNA appear to be a possible *F 'psuedo gene.' So my next question is given the large number of sequences of *F 'unknown' function, how does one tell if the protein produced is from a psuedo *F gene? Secondly, is there an entry or a compiled list of translated cDNA which *F are of 'unknown' function, but have been verified not to be a psuedo gene *F product? *F . *F . *F . *F Thanks for your assistance. You are doing a great job considering how much *F data is being generated. *F Sincerely, *F Thomas L. Selby, Ph. D. *F The Scripps Research Institute *F SR-101 *F 10550 North Torrey Pines Road *F La Jolla, CA 92037 *F Phone: 858-784-9411 *F FAX: 858-784-9483 # *U FBrf0132120 *a Millburn *b G. *t 2000.11.8 *T personal communication to FlyBase *u FlyBase error report for CG6419 on Wed Nov 8 09:24:46 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Nov 08 17:55:57 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 8 Nov 2000 17:55:57 \+0000 *F Date: Wed, 8 Nov 2000 09:24:46 \-0800 (PST) *F From: FlyBase-error@hedgehog.lbl.gov *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG6419 on Wed Nov 8 09:24:46 2000 *F Content-Length: 1431 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG6419 *F Gene annotation error *F Gene CG6419 corresponds to sox-like *F Comments: Hi, *F I believe that CG6419 (FBgn0036413) corresponds to the gene 'sox-like' *F (from FBrf0127030 == Burmester et al., 2000, Gene 246(1-2): 157--167) *F for the following reasons: *F 1. In Figure 2 of FBrf0127030, 'sox-like' is shown as being 25-30kb *F upstream of Fbp-1 and distal to it: *F \+5 0 \-5 \-10 \-15 \-20 \-25 (kb) *F | | | | | | | *F \------------------------------------- *F <-------- <--- *F Fbp-1 sox-like *F N.B. 'sox-like' has homology to the 'SOX subgroup of the HMG proteins' *F (pg.166 of FBrf0127030). *F Fbp-1 corresponds to CG17285. Inspection of GeneSeen of the region *F surrounding CG17285 shows that there is a CG approximately 25kb *F upstream of Fbp-1, transcribed in the correct direction which has *F homology to SOX proteins \- this CG is CG6419. *F 2. In addition, the STS sequence 'Dm1776' is shown within 'sox-like' in *F Figure 2. of FBrf0127030. *F BLAST against the Drosophila genome using the Dm1776 sequence produces *F one hit in the genome that includes all the sequence of Dm1776. This *F hit is within the sequence of the CG6419 gene (BLAST results will *F follow in e-mail to flybase-updates), *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Wed Nov 08 18:25:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 8 Nov 2000 18:25:56 \+0000 *F To: flybase-updates@morgan.harvard.edu *F Subject: re: Error report for CG6419 *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 8 Nov 2000 17:27:34 \+0000 *F Content-Length: 3733 *F Hi, *F here is BLAST results for STS Dm1776 sequence as promised: *F RID: 973702669-3828-8512 *F Query= DM4901.Dm1776 *F (325 letters) *F Database: Drosophila Genome *F 1181 sequences; 122,680,987 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003535.1|AE003535 Drosophila melanogaster genomic scaf... 591 e-168 *F Alignments *F >gb|AE003535.1|AE003535 Drosophila melanogaster genomic scaffold *F 142000013386050 section 22 of *F 54, complete sequence *F Length = 278748 *F Score = 591 bits (298), Expect = e-168 *F Identities = 319/325 (98%), Gaps = 1/325 (0%) *F Strand = Plus / Minus *F Query: 1 acttacccagccttttggatatctctgaattgtgcatcttaggattatcctgagctatct 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 243883 acttacccagccttttggatatctctgaattgtgcatcttaggattatcctgagctatct *F 243824 *F Query: 61 tgcgccgctgaagtctcgaccagaccatgaaagcgtgcattggacgttttatgtgctcct 120 *F |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| *F Sbjct: 243823 tgcgccgctgaagtctcgaccagaccatgaaagcgttcattggacgttttatgtgctcct *F 243764 *F Query: 121 cgnttgggcgctttgttgtgttctggatcatcagactaaagaagacatcgctgagaaata 180 *F || || |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 243763 cgttttggcgctttgttgtgttctggatcatcagactaaagaagacatcgctgagaaata *F 243704 *F Query: 181 ataggatagaaacgtgtgaatttaatataattctttacaagatgacatacaaaacttgtt 240 *F |||||||||||||||||||||||||| ||| |||||||||||||||||||||||||||| *F Sbjct: 243703 ataggatagaaacgtgtgaatttaat-taaatctttacaagatgacatacaaaacttgta *F 243645 *F Query: 241 tgcatgtaaaataatcttataaatcacgccacataattttaaaaacagttagttcatttg 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 243644 tgcatgtaaaataatcttataaatcacgccacataattttaaaaacagttagttcatttg *F 243585 *F Query: 301 aaattactcacctcgcgctgcttcc 325 *F ||||||||||||||||||||||||| *F Sbjct: 243584 aaattactcacctcgcgctgcttcc 243560 *F Score = 52.0 bits (26), Expect = 8e-06 *F Identities = 71/86 (82%) *F Strand = Plus / Minus *F Query: 18 gatatctctgaattgtgcatcttaggattatcctgagctatcttgcgccgctgaagtctc 77 *F ||||| || ||||||||||| || ||||| ||||| || || ||||| ||||| | | *F Sbjct: 262343 gatatttcagaattgtgcatttttggattgtcctgggccattttgcgacgctgcccccgc *F 262284 *F Query: 78 gaccagaccatgaaagcgtgcattgg 103 *F ||||||||||||||||||| |||||| *F Sbjct: 262283 gaccagaccatgaaagcgttcattgg 262258 *F Score = 50.1 bits (25), Expect = 3e-05 *F Identities = 82/101 (81%) *F Strand = Plus / Minus *F Query: 15 ttggatatctctgaattgtgcatcttaggattatcctgagctatcttgcgccgctgaagt 74 *F ||||| ||||| || ||||||||||| || ||||||| || |||| || ||||| || *F Sbjct: 192146 ttggagatctcagagttgtgcatcttggggttatccttggcgatctgacgacgctgcagg *F 192087 *F Query: 75 ctcgaccagaccatgaaagcgtgcattggacgttttatgtg 115 *F | |||||||||||||| |||| ||||||||| || ||||| *F Sbjct: 192086 cgggaccagaccatgaacgcgttcattggacgcttgatgtg 192046 # *U FBrf0132121 *a Millburn *b G. *t 2000.11.9 *T personal communication to FlyBase *u FlyBase error report for CG7901 on Thu Nov 9 05:21:42 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Nov 09 13:22:02 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 9 Nov 2000 13:22:02 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 9 Nov 2000 05:21:42 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG7901 on Thu Nov 9 05:21:42 2000 *F Content-Length: 1991 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG7901 *F Gene annotation error *F Gene CG7901 corresponds to AJ277140 (PP2A-B') *F Comments: PP2A-B' (accession number AJ277140, RNA sequence) corresponds to *F CG7901 *F (FBgn0038572) and also to CG7913 (FBgn0038573), because: *F 1. BLAST of whole genome sequence using AJ277140 overlaps with the *F region of the genome that includes CG7901 AND CG7913. *F 2. ClustalW alignment of CDS sequences of AJ277140 and CG7901 (both *F isoforms) gives a good alignment \- basically the whole of either *F isoform of CG7901 overlaps with the first 190 approx amino acid *F residues of the AJ277140 (PP2A-B') sequence. *F Nucleotides 1-650 approx of AJ277140 (PP2A-B') align with nucleotides *F 290-930 approx of CG7901 (CG7901 is 937 nucleotides long). *F I am therefore confident that CG7901 corresponds to the N-terminal part *F of PP2A-B', although the annotation of the CG7901 transcript is *F different from the structure of the relevant part of the PP2A-B' *F transcript. *F 3. I think that CG7913 corresponds to the C-terminal part of *F PP2A-B'. *F a. there is good nucleotide ClustalW alignment of nucleotides 650-2250 *F approx of AJ277140 (PP2A-B') with nucleotides 1680-3349 approx of *F CG7913 (CG7913 is 3349bp long) , suggesting that CG7913 corresponds to *F the C-terminal part of PP2A-B'. Also CG7913 is annotated as a protein *F phosphatase. *F I did a BLAST of the CDS sequence of PP2A-B' (Q9NFM7) and amino acid *F residues 190-369 approx of PP2A-B' align with amino acid residues *F 470-650 approx of CG7913 (CG7913 is 661 amino acids long). *F The transcript \+ CDS structure of CG7913 differs from that of the *F relevant part of PP2A-B'. *F CG7901 \+ CG7913 therefore need merging in to one annotation (which *F corresponds to PP2A-B'), and the intron/exon structure may well need *F changing, *F ClustalW alignments \+ BLAST results will follow in e-mail to *F flybase-updates. *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs *F From gm119@gen.cam.ac.uk Thu Nov 09 13:24:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 9 Nov 2000 13:24:49 \+0000 *F To: flybase-updates@morgan.harvard.edu *F Subject: re: FlyBase error report for CG7901 on Thu Nov 9 05:21:42 2000 *F Content-Type: X-sun-attachment *F From: Gillian Millburn (Genetics) *F Date: Thu, 9 Nov 2000 13:24:17 \+0000 *F Content-Length: 54995 *F here are ClustalW alignments \+ BLAST for error report, *F Gillian *F Pairwise Scores: *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: DME277140 2250 bp *F Sequence 2: >CG7913|FBgn0038573|cDNA 3349 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 70 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/933704.603436-16722.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:35637 *F Alignment Score 13114 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/933704.603436-16722.aln *F Your guide tree: *F 933704.603436-16722.dnd *F (DME277140:0.14644,>CG7913|FBgn0038573|cDNA:0.14644); *F Your Multiple Sequence Alignment: *F 933704.603436-16722.aln *F CLUSTAL W (1.81) multiple sequence alignment *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F ACCGTGAACGTCGTGTGCCCCGAAAACTTTAATATTAGTTGAAAATATTC 50 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GTTTCCCAAGTTAATTGCTCTCCTATCCTATTCCCTCATTTGTTCCCATT 100 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F CGAACCATTCTATAGTTTCATACTTAGCGTGCCCAAATTGAGTTTCTAGT 150 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GTAGTCTTTCTCATGTGTTGTTGTTGGTCGGCGGGGGCGGCTGGTAAAAA 200 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F AAACAAGCGTTCCGTATCAAGATTAAAACCGAGTGCCATTTGCTCCAAGT 250 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GCCGGCACTAATGAGATGGTCTTCGGTGCTATGTTGCTGACGGGCAACGG 300 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GCTCAAAGGTCCCAAACAGCAGCAACAGCAACATCAGCAGGAGCCGCAGC 350 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F AGCAGCAGCAGCAATCGCAACAGCAGCAGCAAGAGCAAAAGAAACCGGCG 400 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F CCCATTAAATCCTCCAGCAAGGAGCAGGAAACGCAGCCCATTGCAGCCGG 450 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GTATTATGCCCTCGGCATACGCATACCCAAATCTCCATCGTTTCACAGCG 500 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GCCTAGATCAGCTGGCCGACGACGAGTTGGACGATCAGGTGCAGGATCAG 550 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F CAGCAGGAGCAGCAACAGACCGGCAGCCGATTCAGGTTTAGCAGCGTGGA 600 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GGAGCTAAAGGCCAAGTTCGAGGGGCGGAAAACGCCCCTCTCACCGCCAG 650 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F AGGCAGCAGCAGGAGGAGCAGCCGGATCCGGAGCACCCACCACATCCTCC 700 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F TCACCATCGTCCTCGTCCACCAGCTCCAATTCGTCGGCCTCGGCCCTGTC 750 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F CTCGCTTTCACAGGCGGCCTCATCCTCGCTAGCCTCGGCGGCTGCTAACT 800 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F CCTCGGCCTCATCCTCGGCCGCCACATACGGTGGTGGAGCGAATTCGGCT 850 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F GCAGCTGCACTGATTGCCTCCTCGCCATTTGGATCGCAGTCCTCCACTTC 900 *F DME277140 *F \-------------------------------------------------- *F >CG7913|FBgn0038573|cDNA *F TTCGCTCTCGCTATCCTCGAATGCCGGCATGTCGAAGAACGCGGCAGGAG 950 *F DME277140 *F \-------------------------------------------TCGGTAT 7 *F >CG7913|FBgn0038573|cDNA *F CAGGATCTGGATCTGGATCTGGAACAACGTCAACGGGCGCTGCTAGCGGC 1000 *F \* \* *F DME277140 *F AAAAAACCAAAAACACACATAGAGG-AGAGCACATCCAAAGAGCCAAAAA 56 *F >CG7913|FBgn0038573|cDNA *F AGCAATTCACTTGTGTCCACTTTGGCACAACATTTCTCGGCGGCCACATC 1050 *F \**** \* *F DME277140 *F GAGT-CAGAACCGATTGCG--TATTATGGATAACGAGGCGTTAGATCCAA 103 *F >CG7913|FBgn0038573|cDNA *F CGCCGCAGCATCGGCGGCAGCCGCTGCGGCATCCTCTGCCTCGGCGGCGT 1100 *F \* \* \* *F DME277140 *F CAATAAAAAGCAGTACGTCGGCAGCGAC-GCCAACAGCAGCAGCATCAGA 152 *F >CG7913|FBgn0038573|cDNA *F CCTCCTCGTCAGCTTCCTCCTCATCCACCGCCACCAACAACGCAGCCAGC 1150 *F DME277140 *F AACGACAACAACAGCAGCATCTTCAGTTGTGGAAACCACAACAACAATCG 202 *F >CG7913|FBgn0038573|cDNA *F AGCTCCATCGCCGCCAGCAAGT-CACCTGTGAGCGCGGCCGCGGCCATAA 1199 *F \* \* \** \* \* \***** \* \** \**** \* *F DME277140 *F CGG-CGGCCACAGCCTCAGCGGCAGAATCAAAA--AACGAAACAACGGCC 249 *F >CG7913|FBgn0038573|cDNA *F AGAACATACTGAATGCCACCAAGAGTGTGGGTACTAGTGCTGCGGCGGCG 1249 *F \* \* \* \* \** \* \** \* \* \* *F DME277140 *F ACAAACAACAATAGCAACACAAGCGGCAGCATCAGCAGTAGTAGCAGCAA 299 *F >CG7913|FBgn0038573|cDNA *F GCAG-CAGCGGCGGCCACCTCCTCCTCATCATCCCCATCCTCTGCGGCGG 1298 *F DME277140 *F CAATATAGTCAT--ACCGGCATCG-----GCCACTAACGGTATCA--AAG 340 *F >CG7913|FBgn0038573|cDNA *F CTGTGCCGTCGTCCGCCGCCACCGCCGTCGCCGCTGTTCCCACCACCAGC 1348 *F \* \** \* *F DME277140 *F AGAGTAACAGTAACTTAAGTACAAC-AACAACAGC--AGCAGCAGT--AG 385 *F >CG7913|FBgn0038573|cDNA *F GATGTGCCCGCAGAGGAGATGCGTCCGACGGTGGCCCAGAATCTGGTGGG 1398 *F \** \* \* \* \* *F DME277140 *F CGGCAGCAACAACTGTAGAAGGAGTAGCTCCAGCAATAACGTC-CACAAT 434 *F >CG7913|FBgn0038573|cDNA *F CGGCATTAGCATCTCATTGGGAATTGGCCAGCGCAATGGCGGCGCAGCAC 1448 *F \** \* \** \* *F DME277140 *F CGTGGTGACCGGCGGCACT-----CCTCCATTG--AGCAGTTTGGCTAAC 477 *F >CG7913|FBgn0038573|cDNA *F CTGCGTCATCAGCAGTAATGGTGACACCTAACGCCACCATGGTGGTGACC 1498 *F \* \** \* \* \** \* \* \* \* \* \* \* *F \* \** \*** \* \* *F DME277140 *F AAACTCAAGGACAACACACCACCGTACGATGCACCGCCGCCCACGCCGAT 527 *F >CG7913|FBgn0038573|cDNA *F ACATCGAGCCTAAGCATCCGGCAGAACGGTGACATACTGCC-GGGTCATC 1547 *F \* \* \* \* \** \* \* \* \*** \** \* *F \*** \* \* *F DME277140 *F CAGCAAGGTCCTGAACATCACC---GGCACCCCGATCGTTCGCAAG---- 570 *F >CG7913|FBgn0038573|cDNA *F CGGCGGGTCTTCAGCCCTCGCCCCAGACGCTCCAACAGCTGACGGGCAGT 1597 *F \* \** \* \* \** \** \* \* \* \** \* *F \* \* \* \* *F DME277140 *F \----GAGAAGCGCCAGACCAGCGCCCGGTACA-ATGCCTCCAAGAACTGC 615 *F >CG7913|FBgn0038573|cDNA *F CCGGGAAGGGCTCGCGACCGGAATCTATTCTACACGCCACCCACCGCTTC 1647 *F \** \* \** \* *F DME277140 *F GAAC-TGACGGCCCTCATTCCGCTAAACGAGAAGACCGCCGCCAGTGAAC 664 *F >CG7913|FBgn0038573|cDNA *F GGTTGCCATGGCGCTGCCTGCACT--GCGAGA-GACCGCCGCCAGTGAAC 1694 *F DME277140 *F GTGAGGAGCTGTTCATACAGAAGATCCAGCAATGCTGCACACTGTTCGAC 714 *F >CG7913|FBgn0038573|cDNA *F GTGAGGAGCTGTTCATACAGAAGATCCAGCAATGCTGCACACTGTTCGAC 1744 *F DME277140 *F TTCTCCGAGCCGCTCAGCGACCTCAAGTTCAAGGAGGTGAAGCGGGCGGC 764 *F >CG7913|FBgn0038573|cDNA *F TTCTCCGAGCCGCTCAGCGACCTCAAGTTCAAGGAGGTGAAGCGGGCGGC 1794 *F DME277140 *F TCTGCACGAAATGGTCGATTTCCTCACCAACCAAAATGGCGTAATAACCG 814 *F >CG7913|FBgn0038573|cDNA *F TCTGCACGAAATGGTCGATTTCCTCACCAACCAAAATGGCGTAATAACCG 1844 *F DME277140 *F AAGTTATTTACCCGGAGGCGATCAATATGTTTGCTGTCAACCTTTTCCGA 864 *F >CG7913|FBgn0038573|cDNA *F AAGTTATTTACCCGGAGGCGATCAATATGTTTGCTGTCAACCTTTTCCGA 1894 *F DME277140 *F ACTCTGCCGCCATCTTCCAACCCGAATGGTGCCGAATTCGATCCGGAGGA 914 *F >CG7913|FBgn0038573|cDNA *F ACTCTGCCGCCATCGTCCAACCCGAATGGTGCCGAATTCGATCCGGAGGA 1944 *F DME277140 *F GGATGAGCCCACGTTGGAGTCCTCGTGGCCGCATCTGCAACTCGTTTACG 964 *F >CG7913|FBgn0038573|cDNA *F GGATGAGCCCACGTTGGAGTCCTCGTGGCCGCATCTGCAACTCGTTTACG 1994 *F DME277140 *F AGCTGTTCTTGCGCTTCTTGGAGTCACCAGATTTTCAACCAAGCATGGCA 1014 *F >CG7913|FBgn0038573|cDNA *F AGCTGTTCTTGCGCTTCTTGGAGTCACCAGATTTTCAACCAAGCATGGCA 2044 *F DME277140 *F AAACGTTTTATCGACCATCAATTTGTATTACAACTATTGGATTTATTCGA 1064 *F >CG7913|FBgn0038573|cDNA *F AAACGTTTTATCGACCATCAATTTGTATTACAACTATTGGATTTATTCGA 2094 *F DME277140 *F TTCGGAGGATCCACGTGAACGTGATTTCCTGAAGACTGTTTTACATCGCA 1114 *F >CG7913|FBgn0038573|cDNA *F TTCGGAGGATCCACGTGAACGTGATTTCCTGAAGACTGTTTTACATCGCA 2144 *F DME277140 *F TCTATGGAAAATTTTTGGGCTTGAGAGCATTTATTAGAAAGCAGATCAAC 1164 *F >CG7913|FBgn0038573|cDNA *F TCTATGGAAAATTTTTGGGCTTGAGAGCATTTATTAGAAAGCAGATCAAC 2194 *F DME277140 *F AATGTCTTTTACAGA--------TTTATTTATGAAACGGAGCATCATAAT 1206 *F >CG7913|FBgn0038573|cDNA *F AATGTCTTTTACAGACATTTAGATTTATTTATGAAACGGAGCATCATAAT 2244 *F DME277140 *F GGCATAGCCGAATTGTTGGAAATCCTGGGTAGCATTATCAATGGCTTTGC 1256 *F >CG7913|FBgn0038573|cDNA *F GGCATAGCCGAATTGTTGGAAATCCTGGGTAGCATTATCAATGGCTTTGC 2294 *F DME277140 *F TCTGCCGCTTAAGGAGGAGCATAAACAATTTTTACTTAAGGTATTGCTGC 1306 *F >CG7913|FBgn0038573|cDNA *F TCTGCCGCTTAAGGAGGAGCATAAACAATTTTTACTTAAGGTATTGCTGC 2344 *F DME277140 *F CATTGCACAAAGCCAAGAGCCTCTCGGTCTACCATCCGCAGCTCACCTAT 1356 *F >CG7913|FBgn0038573|cDNA *F CATTGCACAAAGCCAAGAGCCTCTCGGTCTACCATCCGCAGCTCACCTAT 2394 *F DME277140 *F TGTGTGGTGCAGTTCCTGGAGAAGGATCCTAGCTTATCGGAGGCGGTCAT 1406 *F >CG7913|FBgn0038573|cDNA *F TGTGTGGTGCAGTTCCTGGAGAAGGATCCTAGCTTATCGGAGGCGGTCAT 2444 *F DME277140 *F CAA----------------------------------------------- 1409 *F >CG7913|FBgn0038573|cDNA *F CAAGTGAGTAAAGAATTCCTTATTTTGCGCATTTTCTAATTAGCCTATCG 2494 *F DME277140 *F \-------------------------AAGCCTACTTAAATTTTGGCCCAAG 1434 *F >CG7913|FBgn0038573|cDNA *F CTGCCATATTTCAATATTCTTGCAGAAGCCTACTTAAATTTTGGCCCAAG 2544 *F DME277140 *F ACGCACAGTCCCAAGGAGGTTATGTTTTTGAACGAGCTGGAGGAGCTGTT 1484 *F >CG7913|FBgn0038573|cDNA *F ACGCACAGTCCCAAGGAGGTTATGTTTTTGAACGAGCTGGAGGAGCTGTT 2594 *F DME277140 *F GGACGTAATTGAGCCGGCCGAGTTCCAGAAGGTGATGGTGCCGCTGTTCC 1534 *F >CG7913|FBgn0038573|cDNA *F GGACGTAATTGAGCCGGCCGAGTTCCAGAAGGTGATGGTGCCGCTGTTCC 2644 *F DME277140 *F GCCAAATAGCCAAGTGCGTCTCTTCGCCTCATTTCCAGGTGGCCGAACGT 1584 *F >CG7913|FBgn0038573|cDNA *F GCCAAATAGCCAAGTGCGTCTCTTCGCCTCATTTCCAGGTGGCCGAACGT 2694 *F DME277140 *F GCGTTGTACTATTGGAACAACGAGTACATTATGTCGCTGATAACGGATAA 1634 *F >CG7913|FBgn0038573|cDNA *F GCGTTGTACTATTGGAACAACGAGTACATTATGTCGCTGATAACGGATAA 2744 *F DME277140 *F CTCGGCGGTGATATTACCCATTATGTTCCCAGCGCTCAATCGCAACTCAA 1684 *F >CG7913|FBgn0038573|cDNA *F CTCGGCGGTGATATTACCCATTATGTTCCCAGCGCTCAATCGCAACTCAA 2794 *F DME277140 *F AGACGCACTGGAACAAGAACATCCATGGTCTGATCTACAATGCACTCAAG 1734 *F >CG7913|FBgn0038573|cDNA *F AGACGCACTGGAACAAGACCATCCATGGTCTGATCTACAATGCACTCAAG 2844 *F DME277140 *F CTGTTCATGGAGATAGATCAGCGGCTTTTTGACGAGTGCAGCAAGAACTA 1784 *F >CG7913|FBgn0038573|cDNA *F CTGTTCATGGAGATAGATCAGCGGCTTTTTGACGAGTGCAGCAAGAACTA 2894 *F DME277140 *F CAAGCAAGAGAAGCAGATGGAGCGTGAGAAGCTGTCGCAAAGGGAGGAGC 1834 *F >CG7913|FBgn0038573|cDNA *F CAAGCAAGAGAAGCAGATGGAGCGTGAGAAGCTGTCGCAAAGGGAGGAGC 2944 *F DME277140 *F TCTGGCAACAGGTGGAGAGCTTGGCCAAGACCAACCCGGAGTGGACAAAG 1884 *F >CG7913|FBgn0038573|cDNA *F TCTGGCAACAGGTGGAGAGCTTGGCCAAGACCAACCCGGAGTGGACAAAG 2994 *F DME277140 *F GCGCGCCGGTTTAACGACTGCCTGCCGGTCAGCGACAGCCGGGCCCTGTG 1934 *F >CG7913|FBgn0038573|cDNA *F GCGCGCCGGTTTAACGACTGCCTGCCGGTCAGCGACAGCCGGGCCCTGTG 3044 *F DME277140 *F CGATCAATATAGTGAGAACAGCGATTCAGCGTATGATCAGAGCGAGCAGA 1984 *F >CG7913|FBgn0038573|cDNA *F CGATCAATATAGTGAGAACAGCGATTCAGCGTATGATCAGAGCGAGCAGA 3094 *F DME277140 *F GGGCGCGCCAGCCGCCGCCTCCGCTGCCGCCCCAGAAACAGGCGCACCAG 2034 *F >CG7913|FBgn0038573|cDNA *F GGGCGCGCCAGCCGCCGCCTCCGCTGCCGCCACAGAAACAGGCGCACCAG 3144 *F DME277140 *F GAGCCCCGAGAGGTGAGACAGGCACTTGCCACATTAACAACACTAAACAA 2084 *F >CG7913|FBgn0038573|cDNA *F GAGCCCCGAGAGGTGAGACAGGCACTTGCCACATTAACAACACTAAACAA 3194 *F DME277140 *F CTACTAATCCGCCACGTGTGATCCTGGCGTCGCTGTGAGCTGTGCCCAGC 2134 *F >CG7913|FBgn0038573|cDNA *F CTACTAATCCGCCACGTGTGATCCTGGCGTCGCTGTGAGCTGTGCCCAGC 3244 *F DME277140 *F TAAGGCCCACTCTGTTTCAACTCTAATTCTAATCGAACGAATGTCTGTTA 2184 *F >CG7913|FBgn0038573|cDNA *F TAAGGCCCACTCTGTTTCAACTCTAATTCTAATCGAACGAATGTCTGTTA 3294 *F DME277140 *F AAATAATCACACTCATGTCATTTAAAAAAAAAA---TACATATTTGTATA 2231 *F >CG7913|FBgn0038573|cDNA *F AAATAATCACACTCATGTCATTTAAAAAAAAAAAAATACATATTTGTATA 3344 *F DME277140 TCTTTAAAAAAAAAAAAAA 2250 *F >CG7913|FBgn0038573|cDNA TCTTT-------------- 3349 *F \------------------------------------------------------------------------------ *F -- *F Pairwise Scores: *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: DME277140 2250 bp *F Sequence 2: >CG7901|FBgn0038572|cDNA 937 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 68 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/274121.198283-26237.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:12284 *F Alignment Score 4773 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/274121.198283-26237.aln *F Your guide tree: *F 274121.198283-26237.dnd *F (DME277140:0.15582,>CG7901|FBgn0038572|cDNA:0.15582); *F Your Multiple Sequence Alignment: *F 274121.198283-26237.aln *F CLUSTAL W (1.81) multiple sequence alignment *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F GCAACGCCGCCGCACGCCTGCAGCCCTGGTAACTCTATTTTCTATTGAGC 50 *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F ACCGACAACGTTGCGTGTATAAGACAGTTTACATAAATTATTATTTACAA 100 *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F TTGCACAGAGCGTTGATGTTGTGCGTTCTAAGCGAAAAGTGCAAATATAA 150 *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F TTTTCAGATAGCAATCAACTGAGAGCGGTCCAGCCCGGCCTGTTGTATTT 200 *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F GTTTGTTTGTCCGGTTGTTTACGTTTTTTTCTCTCGTTCTCGCTGCTTTT 250 *F DME277140 *F \---------------------------------TCGGTATAAAAAACCAA 17 *F >CG7901|FBgn0038572|cDNA *F CATACGTATTTTATATTTTGTAAAAATAAAAAATCGTTATAAAAAACCAA 300 *F DME277140 *F AAACACACATAGAGGAGAGCACATCCAAAGAGCCAAAAAGAGTCAGAACC 67 *F >CG7901|FBgn0038572|cDNA *F AAACACACATAGAGGAGAGCACATCCAAAGAGCCAAAAAGAGTCAGAACC 350 *F DME277140 *F GATTGCGTATTATGGATAACGAGGCGTTAGATCCAACAATAAAAAGCAGT 117 *F >CG7901|FBgn0038572|cDNA *F GATTGCGTATTATGGATAACGAGGCGTTAGATCCAACAATAAAAAGCAGT 400 *F DME277140 *F ACGTCGGCAGCGACGCCAACAGCAGCAGCATCAGAAACGACAACAACAGC 167 *F >CG7901|FBgn0038572|cDNA *F ACGTCGGCAGCGACGCCAACAGCAGCAGCATCAGAAACGACAACAACAGC 450 *F DME277140 *F AGCATCTTCAGTTGTGGAAACCACAACAACAATCGCGGCGGCCACAGCCT 217 *F >CG7901|FBgn0038572|cDNA *F AGCATCTTCAGTTGTGGAAACCACAACAACAATCGCGGCGGCCACAGCCT 500 *F DME277140 *F CAGCGGCAGAATCAAAAAACGAAACAACGGCCACAAACAACAATAGCAAC 267 *F >CG7901|FBgn0038572|cDNA *F CAGCGGCAGAATCAAAAAACGAAACAACGGCCACAAACAACAATAGCAAC 550 *F DME277140 *F ACAAGCGGCAGCATCAGCAGTAGTAGCAGCAACAATATAGTCATACCGGC 317 *F >CG7901|FBgn0038572|cDNA *F ACAAGCGGCAGCATCAGCAGTAGTAGCAGCAACAATATAGTCATACCGGC 600 *F DME277140 *F ATCGGCCACTAACGGTATCAAAGAGAGTAACAGTAACTTAAGTACAACAA 367 *F >CG7901|FBgn0038572|cDNA *F ATCGGCCACTAACGGTATCAAAGAGAGTAACAGTAACTTAAGTACAACAA 650 *F DME277140 *F CAACAGCAGCAGCAGTAGCGGCAGCAACAACTGTAGAAGGAGTAGCTCCA 417 *F >CG7901|FBgn0038572|cDNA *F CAACAGCAGCAGCAGTAGCGGCAGCAACAACTGTAGAAGGAGTAGCTCCA 700 *F DME277140 *F GCAATAACGTCCACAATCGTGGTGACCGGCGGCACTCCTCCATTGAGCAG 467 *F >CG7901|FBgn0038572|cDNA *F GCAATAACGTCCACAATCGTGGTGACCGGCGGCACTCCTCCATTGAGCAG 750 *F DME277140 *F TTTGGCTAACAAACTCAAGGACAACACACCACCGTACGATGCACCGCCGC 517 *F >CG7901|FBgn0038572|cDNA *F TTTGGCTAACAAACTCAAGGACAACACACCACCGTACGATGCACCGCCGC 800 *F DME277140 *F CCACGCCGATCAGCAAGGTCCTGAACATCACCGGCACCCCGATCGTTCGC 567 *F >CG7901|FBgn0038572|cDNA *F CCACGCCGATCAGCAAGGTCCTGAACATCACCGGCACCCCGATCGTTCGC 850 *F DME277140 *F AAGGAGAAGCGCCAGACCAGCGCCCGGTACAATGCCTCCAAGAACTGCGA 617 *F >CG7901|FBgn0038572|cDNA *F AAGGAGAAGCGCCAGACCAGCGCCCGGTACAATGCCTCCAAGAACTGCGA 900 *F DME277140 *F ACTGACGGCCCTCATTCCGCTAAACGAGAAGACCGCCGCCAGTGAACGTG 667 *F >CG7901|FBgn0038572|cDNA *F ACTGACGGCCCTCATTCCGCTAAACGAGAGTGA-GTAG------------ 937 *F \***************************** \* \* *F DME277140 *F AGGAGCTGTTCATACAGAAGATCCAGCAATGCTGCACACTGTTCGACTTC 717 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TCCGAGCCGCTCAGCGACCTCAAGTTCAAGGAGGTGAAGCGGGCGGCTCT 767 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCACGAAATGGTCGATTTCCTCACCAACCAAAATGGCGTAATAACCGAAG 817 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TTATTTACCCGGAGGCGATCAATATGTTTGCTGTCAACCTTTTCCGAACT 867 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CTGCCGCCATCTTCCAACCCGAATGGTGCCGAATTCGATCCGGAGGAGGA 917 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TGAGCCCACGTTGGAGTCCTCGTGGCCGCATCTGCAACTCGTTTACGAGC 967 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TGTTCTTGCGCTTCTTGGAGTCACCAGATTTTCAACCAAGCATGGCAAAA 1017 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CGTTTTATCGACCATCAATTTGTATTACAACTATTGGATTTATTCGATTC 1067 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GGAGGATCCACGTGAACGTGATTTCCTGAAGACTGTTTTACATCGCATCT 1117 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F ATGGAAAATTTTTGGGCTTGAGAGCATTTATTAGAAAGCAGATCAACAAT 1167 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GTCTTTTACAGATTTATTTATGAAACGGAGCATCATAATGGCATAGCCGA 1217 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F ATTGTTGGAAATCCTGGGTAGCATTATCAATGGCTTTGCTCTGCCGCTTA 1267 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F AGGAGGAGCATAAACAATTTTTACTTAAGGTATTGCTGCCATTGCACAAA 1317 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCCAAGAGCCTCTCGGTCTACCATCCGCAGCTCACCTATTGTGTGGTGCA 1367 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GTTCCTGGAGAAGGATCCTAGCTTATCGGAGGCGGTCATCAAAAGCCTAC 1417 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TTAAATTTTGGCCCAAGACGCACAGTCCCAAGGAGGTTATGTTTTTGAAC 1467 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GAGCTGGAGGAGCTGTTGGACGTAATTGAGCCGGCCGAGTTCCAGAAGGT 1517 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GATGGTGCCGCTGTTCCGCCAAATAGCCAAGTGCGTCTCTTCGCCTCATT 1567 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TCCAGGTGGCCGAACGTGCGTTGTACTATTGGAACAACGAGTACATTATG 1617 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TCGCTGATAACGGATAACTCGGCGGTGATATTACCCATTATGTTCCCAGC 1667 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCTCAATCGCAACTCAAAGACGCACTGGAACAAGAACATCCATGGTCTGA 1717 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TCTACAATGCACTCAAGCTGTTCATGGAGATAGATCAGCGGCTTTTTGAC 1767 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GAGTGCAGCAAGAACTACAAGCAAGAGAAGCAGATGGAGCGTGAGAAGCT 1817 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GTCGCAAAGGGAGGAGCTCTGGCAACAGGTGGAGAGCTTGGCCAAGACCA 1867 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F ACCCGGAGTGGACAAAGGCGCGCCGGTTTAACGACTGCCTGCCGGTCAGC 1917 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GACAGCCGGGCCCTGTGCGATCAATATAGTGAGAACAGCGATTCAGCGTA 1967 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TGATCAGAGCGAGCAGAGGGCGCGCCAGCCGCCGCCTCCGCTGCCGCCCC 2017 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F AGAAACAGGCGCACCAGGAGCCCCGAGAGGTGAGACAGGCACTTGCCACA 2067 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TTAACAACACTAAACAACTACTAATCCGCCACGTGTGATCCTGGCGTCGC 2117 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TGTGAGCTGTGCCCAGCTAAGGCCCACTCTGTTTCAACTCTAATTCTAAT 2167 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CGAACGAATGTCTGTTAAAATAATCACACTCATGTCATTTAAAAAAAAAA 2217 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 TACATATTTGTATATCTTTAAAAAAAAAAAAAA 2250 *F >CG7901|FBgn0038572|cDNA \--------------------------------- *F \------------------------------------------------------------------------------ *F -- *F Pairwise Scores: *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: DME277140 2250 bp *F Sequence 2: >CG7901|FBgn0038572|cDNA 764 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 81 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/94514.17755-491.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:11994 *F Alignment Score 4628 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/94514.17755-491.aln *F Your guide tree: *F 94514.17755-491.dnd *F (DME277140:0.09424,>CG7901|FBgn0038572|cDNA:0.09424); *F Your Multiple Sequence Alignment: *F 94514.17755-491.aln *F CLUSTAL W (1.81) multiple sequence alignment *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F GCAACGCCGCCGCACGCCTGCAGCCCTGGTAACTCTATTTTCTATTGAGC 50 *F DME277140 *F \-------------------------------------------------- *F >CG7901|FBgn0038572|cDNA *F ACCGACAACGTTGCGTGTATAAGACAGTTTACATAAATTATTATTTACAA 100 *F DME277140 *F \------------TCGGTATAAAAAACCAAAAACACACATAGAGGAGAGCA 38 *F >CG7901|FBgn0038572|cDNA *F TTGCACAGAGCGTTGATGTTGTGCGTTCTAAGCGAAAA--GAGGAGAGCA 148 *F \* \* \* \* \** \* \* \* *F DME277140 *F CATCCAAAGAGCCAAAAAGAGTCAGAACCGATTGCGTATTATGGATAACG 88 *F >CG7901|FBgn0038572|cDNA *F CATCCAAAGAGCCAAAAAGAGTCAGAACCGATTGCGTATTATGGATAACG 198 *F DME277140 *F AGGCGTTAGATCCAACAATAAAAAGCAGTACGTCGGCAGCGACGCCAACA 138 *F >CG7901|FBgn0038572|cDNA *F AGGCGTTAGATCCAACAATAAAAAGCAGTACGTCGGCAGCGACGCCAACA 248 *F DME277140 *F GCAGCAGCATCAGAAACGACAACAACAGCAGCATCTTCAGTTGTGGAAAC 188 *F >CG7901|FBgn0038572|cDNA *F GCAGCAGCATCAGAAACGACAACAACAGCAGCATCTTCAGTTGTGGAAAC 298 *F DME277140 *F CACAACAACAATCGCGGCGGCCACAGCCTCAGCGGCAGAATCAAAAAACG 238 *F >CG7901|FBgn0038572|cDNA *F CACAACAACAATCGCGGCGGCCACAGCCTCAGCGGCAGAATCAAAAAACG 348 *F DME277140 *F AAACAACGGCCACAAACAACAATAGCAACACAAGCGGCAGCATCAGCAGT 288 *F >CG7901|FBgn0038572|cDNA *F AAACAACGGCCACAAACAACAATAGCAACACAAGCGGCAGCATCAGCAGT 398 *F DME277140 *F AGTAGCAGCAACAATATAGTCATACCGGCATCGGCCACTAACGGTATCAA 338 *F >CG7901|FBgn0038572|cDNA *F AGTAGCAGCAACAATATAGTCATACCGGCATCGGCCACTAACGGTATCAA 448 *F DME277140 *F AGAGAGTAACAGTAACTTAAGTACAACAACAACAGCAGCAGCAGTAGCGG 388 *F >CG7901|FBgn0038572|cDNA *F AGAGAGTAACAGTAACTTAAGTACAACAACAACAGCAGCAGCAGTAGCGG 498 *F DME277140 *F CAGCAACAACTGTAGAAGGAGTAGCTCCAGCAATAACGTCCACAATCGTG 438 *F >CG7901|FBgn0038572|cDNA *F CAGCAACAACTGTAGAAGGAGTAGCTCCAGCAATAACGTCCACAATCGTG 548 *F DME277140 *F GTGACCGGCGGCACTCCTCCATTGAGCAGTTTGGCTAACAAACTCAAGGA 488 *F >CG7901|FBgn0038572|cDNA *F GTGACCGGCGGCACTCCTCCATTGAGCAGTTTGGCTAACAAACTCAAGGA 598 *F DME277140 *F CAACACACCACCGTACGATGCACCGCCGCCCACGCCGATCAGCAAGGTCC 538 *F >CG7901|FBgn0038572|cDNA *F CAACACACCACCGTACGATGCACCGCCGCCCACGCCGATCAGCAAGGTCC 648 *F DME277140 *F TGAACATCACCGGCACCCCGATCGTTCGCAAGGAGAAGCGCCAGACCAGC 588 *F >CG7901|FBgn0038572|cDNA *F TGAACATCACCGGCACCCCGATCGTTCGCAAGGAGAAGCGCCAGACCAGC 698 *F DME277140 *F GCCCGGTACAATGCCTCCAAGAACTGCGAACTGACGGCCCTCATTCCGCT 638 *F >CG7901|FBgn0038572|cDNA *F GCCCGGTACAATGCCTCCAAGAACTGCGAACTGACGGCCCTCATTCCGCT 748 *F DME277140 *F AAACGAGAAGACCGCCGCCAGTGAACGTGAGGAGCTGTTCATACAGAAGA 688 *F >CG7901|FBgn0038572|cDNA *F AAACGAGAGTGA-GTAG--------------------------------- 764 *F \******** \* \* *F DME277140 *F TCCAGCAATGCTGCACACTGTTCGACTTCTCCGAGCCGCTCAGCGACCTC 738 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F AAGTTCAAGGAGGTGAAGCGGGCGGCTCTGCACGAAATGGTCGATTTCCT 788 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CACCAACCAAAATGGCGTAATAACCGAAGTTATTTACCCGGAGGCGATCA 838 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F ATATGTTTGCTGTCAACCTTTTCCGAACTCTGCCGCCATCTTCCAACCCG 888 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F AATGGTGCCGAATTCGATCCGGAGGAGGATGAGCCCACGTTGGAGTCCTC 938 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GTGGCCGCATCTGCAACTCGTTTACGAGCTGTTCTTGCGCTTCTTGGAGT 988 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CACCAGATTTTCAACCAAGCATGGCAAAACGTTTTATCGACCATCAATTT 1038 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GTATTACAACTATTGGATTTATTCGATTCGGAGGATCCACGTGAACGTGA 1088 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TTTCCTGAAGACTGTTTTACATCGCATCTATGGAAAATTTTTGGGCTTGA 1138 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GAGCATTTATTAGAAAGCAGATCAACAATGTCTTTTACAGATTTATTTAT 1188 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GAAACGGAGCATCATAATGGCATAGCCGAATTGTTGGAAATCCTGGGTAG 1238 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CATTATCAATGGCTTTGCTCTGCCGCTTAAGGAGGAGCATAAACAATTTT 1288 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TACTTAAGGTATTGCTGCCATTGCACAAAGCCAAGAGCCTCTCGGTCTAC 1338 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CATCCGCAGCTCACCTATTGTGTGGTGCAGTTCCTGGAGAAGGATCCTAG 1388 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CTTATCGGAGGCGGTCATCAAAAGCCTACTTAAATTTTGGCCCAAGACGC 1438 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F ACAGTCCCAAGGAGGTTATGTTTTTGAACGAGCTGGAGGAGCTGTTGGAC 1488 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GTAATTGAGCCGGCCGAGTTCCAGAAGGTGATGGTGCCGCTGTTCCGCCA 1538 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F AATAGCCAAGTGCGTCTCTTCGCCTCATTTCCAGGTGGCCGAACGTGCGT 1588 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TGTACTATTGGAACAACGAGTACATTATGTCGCTGATAACGGATAACTCG 1638 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCGGTGATATTACCCATTATGTTCCCAGCGCTCAATCGCAACTCAAAGAC 1688 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCACTGGAACAAGAACATCCATGGTCTGATCTACAATGCACTCAAGCTGT 1738 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TCATGGAGATAGATCAGCGGCTTTTTGACGAGTGCAGCAAGAACTACAAG 1788 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CAAGAGAAGCAGATGGAGCGTGAGAAGCTGTCGCAAAGGGAGGAGCTCTG 1838 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCAACAGGTGGAGAGCTTGGCCAAGACCAACCCGGAGTGGACAAAGGCGC 1888 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCCGGTTTAACGACTGCCTGCCGGTCAGCGACAGCCGGGCCCTGTGCGAT 1938 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CAATATAGTGAGAACAGCGATTCAGCGTATGATCAGAGCGAGCAGAGGGC 1988 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCGCCAGCCGCCGCCTCCGCTGCCGCCCCAGAAACAGGCGCACCAGGAGC 2038 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F CCCGAGAGGTGAGACAGGCACTTGCCACATTAACAACACTAAACAACTAC 2088 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F TAATCCGCCACGTGTGATCCTGGCGTCGCTGTGAGCTGTGCCCAGCTAAG 2138 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F GCCCACTCTGTTTCAACTCTAATTCTAATCGAACGAATGTCTGTTAAAAT 2188 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 *F AATCACACTCATGTCATTTAAAAAAAAAATACATATTTGTATATCTTTAA 2238 *F >CG7901|FBgn0038572|cDNA *F \-------------------------------------------------- *F DME277140 AAAAAAAAAAAA 2250 *F >CG7901|FBgn0038572|cDNA \------------ *F \------------------------------------------------------------------------------ *F -- *F PROTEIN: *F Pairwise Scores: *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: Q9NFM7.PP2A_B' 670 aa *F Sequence 2: CG7901|FBgn0038572 191 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 98 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/929511.617713-32028.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:3995 *F Alignment Score 1033 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/929511.617713-32028.aln *F Your guide tree: *F 929511.617713-32028.dnd *F (Q9NFM7.PP2A_B':0.00524,CG7901|FBgn0038572:0.00524); *F Your Multiple Sequence Alignment: *F 929511.617713-32028.aln *F CLUSTAL W (1.81) multiple sequence alignment *F Q9NFM7.PP2A_B' *F MDNEALDPTIKSSTSAATPTAAASETTTTAASSVVETTTTIAAATASAAESKNETTATNN 60 *F CG7901|FBgn0038572 *F MDNEALDPTIKSSTSAATPTAAASETTTTAASSVVETTTTIAAATASAAESKNETTATNN 60 *F Q9NFM7.PP2A_B' *F NSNTSGSISSSSSNNIVIPASATNGIKESNSNLSTTTTAAAVAAATTVEGVAPAITSTIV 120 *F CG7901|FBgn0038572 *F NSNTSGSISSSSSNNIVIPASATNGIKESNSNLSTTTTAAAVAAATTVEGVAPAITSTIV 120 *F Q9NFM7.PP2A_B' *F VTGGTPPLSSLANKLKDNTPPYDAPPPTPISKVLNITGTPIVRKEKRQTSARYNASKNCE 180 *F CG7901|FBgn0038572 *F VTGGTPPLSSLANKLKDNTPPYDAPPPTPISKVLNITGTPIVRKEKRQTSARYNASKNCE 180 *F Q9NFM7.PP2A_B' *F LTALIPLNEKTAASEREELFIQKIQQCCTLFDFSEPLSDLKFKEVKRAALHEMVDFLTNQ 240 *F CG7901|FBgn0038572 *F LTALIPLNE----SE--------------------------------------------- 191 *F Q9NFM7.PP2A_B' *F NGVITEVIYPEAINMFAVNLFRTLPPSSNPNGAEFDPEEDEPTLESSWPHLQLVYELFLR 300 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' *F FLESPDFQPSMAKRFIDHQFVLQLLDLFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQ 360 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' *F INNVFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKQFLLKVLLPLHKAKSLSVY 420 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' *F HPQLTYCVVQFLEKDPSLSEAVIKSLLKFWPKTHSPKEVMFLNELEELLDVIEPAEFQKV 480 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' *F MVPLFRQIAKCVSSPHFQVAERALYYWNNEYIMSLITDNSAVILPIMFPALNRNSKTHWN 540 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' *F KNIHGLIYNALKLFMEIDQRLFDECSKNYKQEKQMEREKLSQREELWQQVESLAKTNPEW 600 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' *F TKARRFNDCLPVSDSRALCDQYSENSDSAYDQSEQRARQPPPPLPPQKQAHQEPREVRQA 660 *F CG7901|FBgn0038572 *F \------------------------------------------------------------ *F Q9NFM7.PP2A_B' LATLTTLNNY 670 *F CG7901|FBgn0038572 \---------- *F \------------------------------------------------------------------------------ *F -- *F airwise Scores: *F CLUSTAL W (1.81) Multiple Sequence Alignments *F Sequence format is Pearson *F Sequence 1: Q9NFM7.PP2A_B' 670 aa *F Sequence 2: CG7901|FBgn0038572.alt2 191 aa *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 98 *F Guide tree file created: *F /net/nfs0/vol1/production/w3nobody/tmp/977453.525083-31845.dnd *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:3995 *F Alignment Score 1033 *F CLUSTAL-Alignment file created *F /net/nfs0/vol1/production/w3nobody/tmp/977453.525083-31845.aln *F Your guide tree: *F 977453.525083-31845.dnd *F (Q9NFM7.PP2A_B':0.00524,CG7901|FBgn0038572.alt2:0.00524); *F Your Multiple Sequence Alignment: *F 977453.525083-31845.aln *F CLUSTAL W (1.81) multiple sequence alignment *F Q9NFM7.PP2A_B' *F MDNEALDPTIKSSTSAATPTAAASETTTTAASSVVETTTTIAAATASAAE 50 *F CG7901|FBgn0038572.alt2 *F MDNEALDPTIKSSTSAATPTAAASETTTTAASSVVETTTTIAAATASAAE 50 *F Q9NFM7.PP2A_B' *F SKNETTATNNNSNTSGSISSSSSNNIVIPASATNGIKESNSNLSTTTTAA 100 *F CG7901|FBgn0038572.alt2 *F SKNETTATNNNSNTSGSISSSSSNNIVIPASATNGIKESNSNLSTTTTAA 100 *F Q9NFM7.PP2A_B' *F AVAAATTVEGVAPAITSTIVVTGGTPPLSSLANKLKDNTPPYDAPPPTPI 150 *F CG7901|FBgn0038572.alt2 *F AVAAATTVEGVAPAITSTIVVTGGTPPLSSLANKLKDNTPPYDAPPPTPI 150 *F Q9NFM7.PP2A_B' *F SKVLNITGTPIVRKEKRQTSARYNASKNCELTALIPLNEKTAASEREELF 200 *F CG7901|FBgn0038572.alt2 *F SKVLNITGTPIVRKEKRQTSARYNASKNCELTALIPLNE----SE----- 191 *F Q9NFM7.PP2A_B' *F IQKIQQCCTLFDFSEPLSDLKFKEVKRAALHEMVDFLTNQNGVITEVIYP 250 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F EAINMFAVNLFRTLPPSSNPNGAEFDPEEDEPTLESSWPHLQLVYELFLR 300 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F FLESPDFQPSMAKRFIDHQFVLQLLDLFDSEDPRERDFLKTVLHRIYGKF 350 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F LGLRAFIRKQINNVFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEH 400 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F KQFLLKVLLPLHKAKSLSVYHPQLTYCVVQFLEKDPSLSEAVIKSLLKFW 450 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F PKTHSPKEVMFLNELEELLDVIEPAEFQKVMVPLFRQIAKCVSSPHFQVA 500 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F ERALYYWNNEYIMSLITDNSAVILPIMFPALNRNSKTHWNKNIHGLIYNA 550 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F LKLFMEIDQRLFDECSKNYKQEKQMEREKLSQREELWQQVESLAKTNPEW 600 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' *F TKARRFNDCLPVSDSRALCDQYSENSDSAYDQSEQRARQPPPPLPPQKQA 650 *F CG7901|FBgn0038572.alt2 \-------------------------------------------------- *F Q9NFM7.PP2A_B' HQEPREVRQALATLTTLNNY 670 *F CG7901|FBgn0038572.alt2 \-------------------- *F \------------------------------------------------------------------------------ *F -------- *F OK *F BLASTP 2.0a19MP-WashU 05-Feb-1998 Build sol2.5-ultra 01:47:24 05-Feb-1998 *F Reference: Gish, Warren (1994-1997). unpublished. *F Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J. *F Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215:403-10. *F Query= Q9NFM7, 670 bases, 8FF7E193 checksum. *F (670 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG5643|FBan0005643|CT17830|FBan0005643 last_updated:000321 1505 9.3e-156 1 *F CG7913|FBan0007913|CT23900|FBan0007913 last_updated:000321 931 6.2e-95 1 *F CG4724|FBan0004724|CT15179|FBan0004724 last_updated:000321 452 8.0e-44 1 *F CG7901|FBan0007901|CT23882|FBan0007901 last_updated:000321 314 3.5e-29 1 *F CG7901|FBan0007901|CT42545|FBan0007901 last_updated:000321 314 3.5e-29 1 *F >CG7913|FBan0007913|CT23900|FBan0007913 last_updated:000321 *F Length = 661 *F Score = 931 (327.7 bits), Expect = 6.2e-95, P = 6.2e-95 *F Identities = 185/214 (86%), Positives = 192/214 (89%) *F Query: 156 ITGTPIVRKEKRQTSARYNASKNCELTALIPLNEKTAASEREELFIQKIQQCCTLFDFSE 215 *F \+TG+P \+++ AS L AL \+TAASEREELFIQKIQQCCTLFDFSE *F Sbjct: 441 LTGSPGRARDRNLFYTPPTASVAMALPAL----RETAASEREELFIQKIQQCCTLFDFSE 496 *F Query: 216 PLSDLKFKEVKRAALHEMVDFLTNQNGVITEVIYPEAINMFAVNLFRTLPPSSNPNGAEF 275 *F PLSDLKFKEVKRAALHEMVDFLTNQNGVITEVIYPEAINMFAVNLFRTLPPSSNPNGAEF *F Sbjct: 497 PLSDLKFKEVKRAALHEMVDFLTNQNGVITEVIYPEAINMFAVNLFRTLPPSSNPNGAEF 556 *F Query: 276 DPEEDEPTLESSWPHLQLVYELFLRFLESPDFQPSMAKRFIDHQFVLQLLDLFDSEDPRE 335 *F DPEEDEPTLESSWPHLQLVYELFLRFLESPDFQPSMAKRFIDHQFVLQLLDLFDSEDPRE *F Sbjct: 557 DPEEDEPTLESSWPHLQLVYELFLRFLESPDFQPSMAKRFIDHQFVLQLLDLFDSEDPRE 616 *F Query: 336 RDFLKTVLHRIYGKFLGLRAFIRKQINNVFYRFI 369 *F RDFLKTVLHRIYGKFLGLRAFIRKQINNVFYR \+ *F Sbjct: 617 RDFLKTVLHRIYGKFLGLRAFIRKQINNVFYRHL 650 # *U FBrf0132122 *a Yamada *b C. *t 2000.11.14 *T personal communication to FlyBase *u FlyBase error report for CG5887 on Thu Nov 9 06:09:05 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Nov 09 14:09:23 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 9 Nov 2000 14:09:23 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 9 Nov 2000 06:09:05 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: cy200@gen.cam.ac.uk *F Subject: FlyBase error report for CG5887 on Thu Nov 9 06:09:05 2000 *F Content-Length: 913 *F Error report from Chihiro Yamada (cy200@gen.cam.ac.uk) *F Gene or accession: CG5887 *F Gene annotation error *F Gene CG5887 should be split. *F Comments: I have reason to believe that CG5887 should be split, based on *F information in the paper: *F FBrf0129781 == Dallerac et al., 2000, Proc. Natl. Acad. Sci. USA 97(17): *F 9449--9454 *F Figure 5 shows two open reading frames for genes Dallerac et al. call *F desat1 and desat2. Both read in the same direction and are separated *F by 3.7kb of sequence including what they call a 'Putative desat1 *F promoter'. Fig 3 shows a protein alignment between the two and these *F correspond to the translations of two accessions AJ245747 & AJ271415, *F (as stated in the figure legend) representing desat1 and desat2 *F respectively. Currently both accessions are attributed to CG5887, *F Fad. *F Chihiro *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132123 *a Rawlings *b N. *t 2000.11.9 *T personal communication to FlyBase *u FlyBase error report on Thu Nov 9 15:56:00 2000. *F From neil.rawlings@bbsrc.ac.uk Thu Nov 09 16:00:15 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 9 Nov 2000 16:00:15 \+0000 *F Date: Thu, 09 Nov 2000 15:56:00 \+0000 *F From: Neil Rawlings *F Organization: Babraham Institute *F X-Mailer: Mozilla 4.6 en (WinNT; I) *F X-Accept-Language: en *F Mime-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F Subject: A new peptidase gene in Drosophila *F Content-Type: multipart/mixed; boundary='------------6794E935177240A8A992E3A4' *F Content-Length: 6451 *F If you are not the right person to contact in respect of annotation of *F the Drosophila genome, then I would be grateful if you would pass this *F message on to the correct person. *F I believe I have discovered a previously unannotated gene in the *F Drosophila genome. Sequence similarity was detected by a TBlastN search *F of the NR database at NCBI using the sequence of the Saccharomyces SUMO *F proteinase (peptidase family C48) as a probe. A match was found with *F GenBank entry AE003540, section 28 of 54 of the genomic scaffold *F 142000013386050 of Drosophila. The Drosophila Sumo homologue occupies *F bases 232218-233453 and is a continuous open reading frame from *F initiation methionine to stop codon: there are no introns. I attach a *F file (Test.Res) showing a TFastX-derived alignment between this *F Drosophila sequence (TEST) and BcDNA:GH02751 (AAD38583 \- another *F Drosophila Sumo homologue), and a file containing the complete amino *F acid sequence of the newly identified gene (AE003540.AA). *F In GenBank entry AE003540 the region 232218-233453 is part of an intron *F to an existing gene (BcDNA:LD21213). Is it possible that this gene has *F been incorrectly assembled? The derived protein sequence is unusually *F Pro, Gln and Gly-rich. *F ============================================================================== *F Dr Neil D. Rawlings *F MRC Molecular Enzymology Laboratory *F Babraham Institute *F Babraham Hall *F Babraham *F Cambridgeshire *F CB2 4AT *F UK *F Tel: \+1223 496649 *F Fax: \+1223 496023 *F mailto:neil.rawlings@bbsrc.ac.uk *F ============================================================================== *F Please visit the MEROPS database, the URL is: *F http://WWW.MEROPS.CO.UK/merops/Merops.htm *F ============================================================================== *F Test.Res: *F TFASTXY compares a protein to a translated DNA data bank *F version 3.2t06 July 29, 1999 *F Please cite: *F Pearson et al, Genomics (1997) 46:24-36 *F aaseq\AAD38583.aa, 674 aa *F vs test.dna library *F 297385 residues in 1 sequences *F TFASTX (3.26 July 1999) function optimized, BL50 matrix (15:-5) ktup: 2 *F join: 38, opt: 26, gap-pen: \-15/ \-2 shift: \-20, width: 16 reg.-scaled *F The best scores are: initn init1 opt *F TEST \- test (99312) f 960 883 888 *F >>TEST \- test (99312 aa) *F Frame: f initn: 960 init1: 883 opt: 888 *F Smith-Waterman score: 934; 47.097% identity in 310 aa overlap *F (365-674:232548-233450) *F 370 380 390 400 410 420 *F AAD385 HRRFANCIFLRNDFAENFKARANRRQLESMHLLGIAEQQANESKDERLAYEKKLREVMFR *F .::: .:::... :.:. ..... . . :. :.: ..: ::.: : . .. *F TEST NRRFRRSLFLRSNYIEQFRRWTDQKNKLNRKRLNEAHQLCLKAKHERIACEIDRYKKLIL *F 232560 232590 232620 232650 232680 232710 *F 430 440 450 460 470 480 *F AAD385 SGAPHRPFFEIGPLEQPEEKKETKLIPLTKEDHARFQEMTTIEVTTNLIFKYNLQITTDD *F . . : . . ..::::::: : :..:.. . .. :.::.: .: *F TEST KQSVH---------VIEDIRISSELIPLTKEHHDRLMELSKYPLQQVIVAKFNLDICGSD *F 232740 232770 232800 232830 232860 *F 490 500 510 520 530 540 *F AAD385 IFTFVDGEWLNDAIINFYMSMLTERSEKRAGELPATYAMNTFFMPRLLQAGYAGVRRWTR *F : ...: :::: ::::::..:.:::::: : .:..:::.:::.:::::.:. ::.:::: *F TEST IKILTSGGWLNDKIINFYMNLLVERSEKRPGTVPSVYAMSTFFVPRLLQSGFDGVKRWTR *F 232890 232920 232950 232980 233010 233040 *F 550 560 570 580 590 600 *F AAD385 KVDLFSKDIIPVPVHCGNVHWCMAIIHLRNKTIFYYDSMGRPNQPALDALVKYLHEESLD *F :::::: :.: :::: ::::..:: : ::..::.: :: . . ::::::. :: : *F TEST KVDLFSMDLILVPVHQMLVHWCLVIIDLPAKTMLYYNSRGRGDPNLMRALVKYLQMESED *F 233070 233100 233130 233160 233190 233220 *F 610 620 630 640 650 660 *F AAD385 KRKQPFDMTGFVVENAQNIPRQGNSSDCGVFSCMFAEYITRDVPITFSQAEMLYFRTKMA *F : .: . : .:.:::.:.: : .::::: ::::::.:::.:::::. .: ::::::. *F TEST KLGLCLDTSEFRIEDAQNVPQQDNMNDCGVFVCMFAEYLTRDAPITFSKKDMKYFRTKMV *F 233250 233280 233310 233340 233370 233400 *F 670 *F AAD385 LEIADGKLWQ *F ::.. .::. *F TEST LELTGDQLWK *F 233430 *F ============================================================================== *F AE003540.AA: *F >AE003540 \- C48 homologue, Drosophila melanogaster *F MLAFEKEIVEKSRYFDLITKECQDYPMAKTMPVLKQPENFQEQEEDVV *F MAVQEKLKFFRKLTSNGPAVPTPLKVVEKEDIGHKEIPKV *F VKKVDRKTKLLQAIPAVFKHSHNRRFRRSLFLRSNYIEQF *F RRWTDQKNKLNRKRLNEAHQLCLKAKHERIACEIDRYKKL *F ILKQSVHVIEDIRISSELIPLTKEHHDRLMELSKYPLQQV *F IVAKFNLDICGSDIKILTSGGWLNDKIINFYMNLLVERSE *F KRPGTVPSVYAMSTFFVPRLLQSGFDGVKRWTRKVDLFSM *F DLILVPVHQMLVHWCLVIIDLPAKTMLYYNSRGRGDPNLM *F RALVKYLQMESEDKLGLCLDTSEFRIEDAQNVPQQDNMND *F CGVFVCMFAEYLTRDAPITFSKKDMKYFRTKMVLELTGDQ *F LWK* *F ============================================================================== # *U FBrf0132124 *a Gatt *b M. *t 2000.11.14 *T personal communication to FlyBase *u FlyBase error report on Tue Nov 14 18:23:46 2000. *F >From mkg23@mole.bio.cam.ac.uk Tue Nov 14 18:14:58 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Tue, 14 Nov 2000 18:14:58 \+0000 *F Date: Tue, 14 Nov 2000 18:23:46 \+0000 *F From: Melanie Gatt *F X-Mailer: Mozilla 4.61 en (WinNT; I) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: Aubrey de Grey *F Subject: Change to FlyBase *F Content-Transfer-Encoding: 7bit *F Aubrey, *F I have noticed that there are seperate entries for futsch and EG:49E4.1 *F in FlyBase. In the paper that the EDGP are submitting we have written *F ... *F Another very large gene is futsch (EG:49E4.1), covering 18 Kb but *F encoding a protein of 5327 amino acids predicted to encode a *F micotubule-associated protein, on the basis of its similarity with human *F MAP1B (SWISS-PROT:P46821) which is only half the size. Recently Hummel *F and colleagues (2000) have shown that futsch encodes the well-known *F Drosophila neural antigen 22C10. *F Hummel, T., K. Krukkert, J. Roos, G. Davis, and C. Klambt. 2000. *F Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic *F and axonal development. Neuron 26: 357-370. *F ... *F cheers *F mel :-) # *U FBrf0132125 *a Scott *b M.J. *t 2000.11.9 *T personal communication to FlyBase *u FlyBase error report on Thu 9 Nov 09:53:42 2000. *F From bdgp-admin@fruitfly.bdgp.berkeley.edu Thu Nov 09 20:56:30 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 9 Nov 2000 20:56:30 \+0000 *F Mime-Version: 1.0 *F X-Sender: MJScott@mail.massey.ac.nz *F Date: Fri, 10 Nov 2000 09:53:42 \+1300 *F To: bdgp@fruitfly.org *F From: Max *F Content-Type: multipart/alternative; *F boundary='============_-1238319272==_ma============' *F Subject: BDGP rolled *F Sender: bdgp-admin@fruitfly.bdgp.berkeley.edu *F X-BeenThere: bdgp@fruitfly.BDGP.Berkeley.EDU *F X-Mailman-Version: 2.0beta6 *F List-Help: *F List-Post: *F List-Subscribe: , *F *F List-Id: BDGP mailing list *F List-Unsubscribe: , *F *F Content-Length: 1574 *F I noticed that the gene CG18732 in AE002642 is identical to *F nucleotides 784-1257 of rolled (gb|M95124.1|DROMAPKIN ) which has *F been called CG12559 in AE003090. *F \-- *F Max Scott PhD *F Senior Lecturer in Genetics *F Institute of Molecular BioSciences *F Massey University *F Private Bag 11222 *F Palmerston North *F New Zealand *F Fax: 64-6-350 5688 *F Phone: 64-6-350 5515 ext 2586 *F E-mail: M.J.Scott@massey.ac.nz *F research summary: http://imbs.massey.ac.nz/HTML/mscott.html # *U FBrf0132126 *a Whitfield *b E. *t 2000.11.17 *T personal communication to FlyBase *u FlyBase error report for CG15519 on Fri Nov 17 08:39:03 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Nov 17 16:39:18 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 17 Nov 2000 16:39:18 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 17 Nov 2000 08:39:03 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG15519 on Fri Nov 17 08:39:03 2000 *F Content-Length: 635 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG15519 *F Gene annotation error *F Gene CG15519 has incorrect exon/intron structure. *F Comments: Celera have introduced an intron into this translation \- there *F should be no *F intron, please compare to Yun and Davis, Mol. Cell. Biol. 9:692-700(1989) *F DR EMBL; M24379; AAB02552 *F DR EMBL; M24379; AAB02553 *F . *F . *F . *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132127 *a Crosby *b M. *t 2000.11.17 *T personal communication to FlyBase *u FlyBase error report for CG6050 on Fri Nov 17 12:29:11 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Nov 17 20:29:16 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 17 Nov 2000 20:29:16 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 17 Nov 2000 12:29:11 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: crosby@morgan.harvard.edu *F Subject: FlyBase error report for CG6050 on Fri Nov 17 12:29:11 2000 *F Content-Length: 438 *F Error report from Madeline Crosby (crosby@morgan.harvard.edu) *F Gene or accession: CG6050 *F Release: 1 *F Gene annotation error *F Genes CG6050 and EfTuM should be merged. *F Comments: Insertion site for P{lacW}EfTuML4569 maps to this gene. Note that *F stock *F for P{lacW}EfTuML4569 is balanced over CyO, despite reported localization *F to 89E. *F Browser: Mozilla/4.61 en (X11; U; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132128 *a Whitfield *b E. *t 2000.11.20 *T personal communication to FlyBase *u FlyBase error report for CG10223 on Mon Nov 20 04:41:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Nov 20 12:41:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 20 Nov 2000 12:41:56 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 20 Nov 2000 04:41:43 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10223 on Mon Nov 20 04:41:43 2000 *F Content-Length: 498 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10223 *F Release: 1 *F Gene annotation error *F Gene CG10223 has incorrect exon/intron structure. *F Comments: Celera define an extra intron in Top2 (AE003663; AAF53802). As the *F intron is *F in frame with the flanking exns readthrough produces a protein 100% identical *F to X61209; CAA43523, Wyckoff et al, J. Mol. Biol. 205:1-13(1989). *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132129 *a Dunkov *b B. *t 2000.10.12 *T personal communication to FlyBase *u FlyBase error report on Thu Oct 12 19:01:18 2000. *F >From dunkov@u.arizona.edu Thu Oct 12 02:59:39 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 12 Oct 2000 02:59:39 \+0100 *F X-Sender: dunkov@dunkov.inbox.email.arizona.edu *F Mime-Version: 1.0 *F Date: Wed, 11 Oct 2000 19:01:18 \-0700 *F To: ma11@gen.cam.ac.uk *F From: Boris Dunkov *F Subject: mitochondrial P450s *F Dear Michael, *F I was checking the ever-improving annotations of the P450s and I have some *F considerations that apply to 11 of them, these that are putative *F mitochondrial enzymes. While I detected the mitochondrial P450s at the *F jamboree and discussed some of them with David Nelson, only now I had the *F time to look at all of them and communicate my observations to FlyBase with *F a suggestion to correct the annotations. I thought that since the evidence *F below applies to all 11 P450s, I would rather write to you and ask you to *F communicate it to the FlyBase annotators (and file it as a personal *F communication if you wish) rather than using 11 'fix annotation' entries. *F In case it is more practical I will do the 'fix annotaions'. *F While most P450s are indeed microsomal, some are mitochondrial. Among the *F fly P450s 11 appear to be good candidates for mitochondrial enzymes. These *F are: all CYP12s \- CYP12A4, CYP12A5, CYP12B2, CYP12C1, CYP12D1, and CYP12E1, *F CYP49A1, CYP301A1, CYP302A1, CYP314A1, and CYP315A1. *F Several lines of evidence suggest that these are putative mitochondrial *F P450s: 1) They are most closely related to mitochondrial P450s from other *F organisms as judged by overall sequence similarity; 2) Presence of a *F putative mitochondrial targeting sequences at their N-termini \- *F amphipathic, rich in basic residues (mainly R and K) in contrast to the *F N-termini of microsomal P450s which are highly hydrophobic; 3) presence of *F a conserved pair of basic residues (with the exception of CYP314A1 where Q *F is present instead of the second basic residue)in the C-terminal half of *F the sequences, probably orthologous to the RxxxK pair of the putative *F adrenodoxin binding site of mammalian mitochondrial P450s. *F Additional strong functional evidence that these are mitochondrial P450s *F derives from two recent studies: *F 1) The first family 12 member, CYP12A1 (from the house fly Musca domestica) *F has been firmly established as a mitochondrial enzyme by localization in *F the mitochondria by immunocytochemistry and by functional characterization *F of the recombinant protein. The later was efficiently reduced only by the *F mitochondrial electron transfer partners adrenodoxin reductase and *F adrenodoxin and not by the microsomal cytochrome P450 reductase (Guzov et *F al., Arch.Biochem.Biophys. 359:231-249, 1998). Thus, the other CYP12s are *F more than likely to be also mitochondrial enzymes. *F 2) In the case of CYP302A1 (disembodied, Chavez et al., Development *F 127:4115-4126, 2000), all biochemical and genetic evidence points to a *F mitochondrial localization of the enzyme. Surprisingly, the GadFly summary *F incorrectly states that 'It encodes a cytochrome P450 (EC:1.14.14.1) *F putatively involved in metabolism which is a component of the microsome.', *F rather than of the mitochondrion (mitochondrial inner membrane). *F The distinction between microsomal and mitochondrial P450s is interesting *F and important and I suggest that it is reflected in the annotations of the *F sequences to avoid confusion \- now they are all listed as microsomal. Let *F me know if further justification of the proposed changes in the annotations *F is needed. *F P.S. I understand that David Nelson has communicated to you the corrections *F he made to the Drosophila P450 protein sequences. They are all correrct now *F on his site and I think it would be best if they are used for the *F annotations in GadFly. I still do not understand how 30 % of the P450 *F protein sequences given by Celera to GenBank are wrong.... *F Best regards, *F Boris *F Boris Dunkov *F Department of Biochemistry and Molecular Biophysics *F University of Arizona *F BSW \#351 *F 1041 E. Lowell St. *F P.O.Box 210088 *F Tucson, AZ 85721-0088 *F Tel: (520)621-3046 *F Fax: (520)621-9288 *F E-mail: dunkov@u.arizona.edu # *U FBrf0132130 *a Whitfield *b E. *t 2000.11.20 *T personal communication to FlyBase *u FlyBase error report for CG1315 on Mon Nov 20 04:52:57 2000. *F From FlyBase-error@hedgehog.lbl.gov Mon Nov 20 12:53:13 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 20 Nov 2000 12:53:13 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 20 Nov 2000 04:52:57 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1315 on Mon Nov 20 04:52:57 2000 *F Content-Length: 532 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1315 *F Release: 1 *F Gene annotation error *F Gene CG1315 has incorrect exon/intron structure. *F Comments: Celera have defined an extra C-terminal exon (AE003732; AAF55811) *F compared to *F the translation from Celniker et al, Submitted (JAN-1999) to the EMBL database, *F sequence of the ANTP region (AE001574; AAD19816). *F With this correction the sequences are identical. *F thanks *F Browser: Mozilla/4.06 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132131 *a Misra *b S. *t 2000.11.18 *T personal communication to FlyBase *u *F From sima@fruitfly.bdgp.berkeley.edu Sun Nov 19 01:11:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 19 Nov 2000 01:11:59 \+0000 *F Date: Sat, 18 Nov 2000 17:11:44 \-0800 (PST) *F From: Sima Misra *F X-Sender: sima@fruitfly *F To: Aubrey de Grey *F cc: curators@morgan.harvard.edu *F Subject: nc5 report, third installment *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='-559023410-851401618-974596304=:21591' *F Content-Length: 40164 *F Hi Aubrey and Gillian, *F Here is another installment of P insertions. Let me know if you *F have any questions about them. *F cheers, *F sima *F >l(2)05642=Uba1 *F p_name l(2)05642 *F name CG1782 *F gene Uba1 *F ct_name CT5340 *F relation inside *F r_orientation \+ *F inside_intron 0 *F inside_exon 1 *F dist5 22 *F dist3 5018 *F [maps at nt 22 of gene in same orientation as gene; EP(2)2375 maps 544bp *F downstream in same gene] *F >l(2)05643=Rpt1 *F p_name l(2)05643 *F name CG1341 *F gene Rpt1 *F ct_name CT3016 *F relation inside *F r_orientation \+ *F inside_intron 0 *F inside_exon 1 *F dist5 8 *F dist3 1389 *F [l(2)05643 and EP(2)2153 inserted at same nucleotide, 8nt downstream of Rpt1 *F gene, a gene nested in an intron of CG17985; insertion in same orientation as *F gene] *F >l(2)05714=CG8886 *F p_name l(2)05714 *F name CG3792 *F gene *F ct_name CT12669 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 6081 *F dist3 7323 *F p_name l(2)05714 *F name CG8891 *F gene *F ct_name CT25526 *F relation front *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 8023 *F dist3 7320 *F CG8886|FBan0008886|CT25498|FBan0008886 last_updated:000321 *F Length = 940 *F Score = 178 bits (90), Expect = 3e-45 *F Identities = 90/90 (100%), Positives = 90/90 (100%) *F Query 1 gcgccgagtaaccgtcattactagacgccagccagctggagagctccggatatttctccc 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct 290 gcgccgagtaaccgtcattactagacgccagccagctggagagctccggatatttctccc 231 *F Query 61 actctgtgcgatagcgttggtggtacagtc 90 *F |||||||||||||||||||||||||||||| *F Sbjct 230 actctgtgcgatagcgttggtggtacagtc 201 *F [not near any annotation by Guochun (not sure why); but by BLAST, inserted in *F opposite orientation at bp 208 of CG8886 (in LD03394.5')] *F >l(2)05836=CG7392=WD-40-family-member *F p_name l(2)05836 *F name CG7392 *F gene WD-40-family-member *F ct_name CT22741 *F relation inside *F r_orientation \+ *F inside_intron 1 *F inside_exon 0 *F dist5 359 *F dist3 7378 *F [l(2)05836 and EP(2)2514 are 1bp away from each other in intron of CG7392; *F l(2)05836 inserted in same orientation as gene, 53bp downstream of end of *F first exon] *F >l(2)05847=CG8732 *F p_name l(2)05847 *F name CG8732 *F gene CG8732 *F ct_name CT25221 *F relation inside *F r_orientation \+ *F inside_intron 1 *F inside_exon 0 *F dist5 3089 *F dist3 12768 *F [l(2)05847 inserted in intron of CG8732, in same orientation as gene, 473bp *F downstream of end of first exon; EP(2)2365 also inserted 286bp downstream of *F l(2)05847 in same intron; plasmid rescue flanking sequence probably needs *F trimming since only bp56-368 match genomic sequence (or polymorphic between *F strains)] *F >l(2)06225=CG6105 *F p_name l(2)06225 *F name CG6105 *F gene *F ct_name CT19171 *F relation inside *F r_orientation \- *F inside_intron 2 *F inside_exon 0 *F dist5 692 *F dist3 782 *F [inserted in opposite orientation in second intron of CG6105] *F >l(2)06270=CG8846=Phas1? *F p_name l(2)06270 *F name CG8846 *F gene Phas1 *F ct_name CT9265 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 20 *F dist3 1163 *F [l(2)06270 in hotspot for insertion: EP(2)2244 at 3396566, EP(2)1010, *F l(2)k00609 (but complemented), EP(2)2266, EP(2)2085, EP(2)2255, EP(2)2429, *F EP(2)2350, l(2)k13506 (but complemented), l(2)06270 at 3396689, EP(2)0818, *F EP(2)2550, and l(2)k07736 at 3396696 (all within 130bp); l(2)06270 only 20bp *F upstream of Phas 1 start in same orientation as gene] *F >l(2)06496=CG9893 *F p_name l(2)06496 *F name CG9893 *F gene CG9893 *F ct_name CT27878 *F relation inside *F r_orientation \+ *F inside_intron 0 *F inside_exon 1 *F dist5 18 *F dist3 622 *F [l(2)06496 inserted in same orientation as gene 18bp downstream of start of *F CG9893 transcription and in LD16285.5'] *F >l(2)06655=? *F [no computed analysis but BLASTing against All fly sequence: 3' sequence *F indicates inserted at 110608 minus orientation of AE003804.1, not near any *F annotation; 5' says inserted at 112619 in minus orientation of AE003804.1; *F polymorphism in strain or multiple inserts] *F 3' sequence: *F Score = 326 (48.9 bits), Expect = 4.2e-08, P = 4.2e-08 *F Identities = 70/76 (92%), Positives = 70/76 (92%), Strand = Minus / Plus *F Query: 76 GCGCCTTAGACCGCTCGGCCACGCTACCATGTCGTAGATTATGTCAAACGCACATGAAGA 17 *F |||||||||||||||||||||||||||||||| |||||||||||||||||| || |||| *F Sbjct: 110533 GCGCCTTAGACCGCTCGGCCACGCTACCATGTTGTAGATTATGTCAAACGCTCAACAAGA 11059 *F 2 *F Query: 16 CACAAGGCTAGGAAGC 1 *F || |||||||||||| *F Sbjct: 110593 GACGAGGCTAGGAAGC 110608 *F 5' sequence: *F Score = 411 (61.7 bits), Expect = 6.1e-12, P = 6.1e-12 *F Identities = 83/84 (98%), Positives = 83/84 (98%), Strand = Minus / Plus *F Query: 84 GGCGACCCTTTTGCCAGTACGCTTGTTGCCAGTTTCAAGTGTTTTTGTTGCACGTTGACA 25 *F ||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 112612 GGCGACCCTTTTGCCGGTACGCTTGTTGCCAGTTTCAAGTGTTTTTGTTGCACGTTGACA 11267 *F 1 *F Query: 24 GTCCGTTAAAGGTGACACAGGCGC 1 *F |||||||||||||||||||||||| *F Sbjct: 112672 GTCCGTTAAAGGTGACACAGGCGC 112695 *F >l(2)06708 inserted in repeat in genome *F >l(2)06850=grh *F [no computed analysis; BLASTN against All shows l(2)06850 inserted in minus *F orientation at 250845 of AE003801.1, in intron of grh in same orientation as *F gene; also in intron of LP11035.5'; may be polymorphism because no match from *F 122 to 249 of flanking sequence to genomic sequence] *F gadfly|SEG:AE003801|gb|AE003801.1|Drosophila melanogaster genomic scaffold *F 142000013386047 section 11 of 52, complete sequence.|AE003801.1 *F GI:7302679 *F Length = 272,650 *F Minus Strand HSPs: *F Score = 902 (135.3 bits), Expect = 9.1e-56, Sum P(2) = 9.1e-56 *F Identities = 186/192 (96%), Positives = 186/192 (96%), Strand = Minus / Plus *F Query: 441 CGAATTCCCCCAAAGGGAAGTGGNTCGATGTGTGACTGCGATGGTGCTATGTAGCGAGCT 382 *F CGAA TC C CAAAGG AAGTGG TCGATGTGTGACTGCGATGGTGCTATGTAGCGAGCT *F Sbjct: 250407 CGAACTCGCGCAAAGG-AAGTGGTTCGATGTGTGACTGCGATGGTGCTATGTAGCGAGCT 25046 *F 5 *F Query: 381 CTGTGCGGGAGCGAGAGCACCATCCAAATCACGTACGCACACATACGATCGTATAACAGC 322 *F CTGTGCGGGAGCGAGAGCACCAT CAAATCACGTACGCACACATACGATCGTATAACAGC *F Sbjct: 250466 CTGTGCGGGAGCGAGAGCACCATACAAATCACGTACGCACACATACGATCGTATAACAGC 25052 *F 5 *F Query: 321 GTGCGAGCGTATAACAGTTGACCGTGCAGTGGCAGCAGCAAAGGCTCATTGTTGTTGTTG 262 *F GTGCGAGCGTATAACAGTTGACCGTGCAGTGGCAGCAGCAAAGGCTCATTGTTGTTGTTG *F Sbjct: 250526 GTGCGAGCGTATAACAGTTGACCGTGCAGTGGCAGCAGCAAAGGCTCATTGTTGTTGTTG 25058 *F 5 *F Query: 261 CTCGCGTACATA 250 *F CTCGCGTACATA *F Sbjct: 250586 CTCGCGTACATA 250597 *F Score = 600 (90.0 bits), Expect = 9.1e-56, Sum P(2) = 9.1e-56 *F Identities = 120/120 (100%), Positives = 120/120 (100%), Strand = Minus / Plus *F Query: 121 ACGCTGATTCGTATTGGCTGCAGCCACTAATACTCATTGCTCACTCACACCAGCAATTGA 62 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 250726 ACGCTGATTCGTATTGGCTGCAGCCACTAATACTCATTGCTCACTCACACCAGCAATTGA 25078 *F 5 *F Query: 61 CAGACCAATTGCAGGTCTGTTAGACAACAGCAGCGGCAGCAACAACTACATCGCCCAGCA 2 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 250786 CAGACCAATTGCAGGTCTGTTAGACAACAGCAGCGGCAGCAACAACTACATCGCCCAGCA 25084 *F 5 *F >l(2)06949=CG8151? *F p_name l(2)06949 *F name CG8151 *F gene CG8151 *F ct_name CT24236 *F relation behind *F r_orientation \- *F inside_intron 0 *F inside_exon 0 *F dist5 35 *F dist3 2283 *F [l(2)06949 inserted 35bp upstream of CG8151 in opposite orientation of gene] *F >l(2)07129=CG10941=mm? *F p_name l(2)07129 *F name CG10941 *F gene mm *F ct_name CT30649 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 61 *F dist3 96016 *F [l(2)07129 inserted just 61bp upstream of start of mm; hotspot since same *F insertion position as l(2)k07110 (but complemented) and l(2)k04222b (but *F complemented)] *F >l(2)07806=? *F [no computed analysis but BLASTN against All indicated inserted in reverse *F orientation relative to AE003452.1 around 193215, not near any annotations] *F gadfly|SEG:AE003452|gb|AE003452.1|Drosophila melanogaster genomic scaffold *F GI:7291191 *F Length = 305,505 *F Minus Strand HSPs: *F Score = 1299 (194.9 bits), Expect = 4.7e-52, P = 4.7e-52 *F Identities = 267/276 (96%), Positives = 267/276 (96%), Strand = Minus / Plus *F Query: 277 TTGGCAAATTCTTGGTTATAGCACTTCTATAACTTGCATTTCAAATATCTTAAGAGATAG 218 *F || ||||||||||||||||||||||||| |||||||||||||||||||| |||||||||| *F Sbjct: 192940 TTTGCAAATTCTTGGTTATAGCACTTCTTTAACTTGCATTTCAAATATCATAAGAGATAG 19299 *F 9 *F Query: 217 CTAATTAAGTCTTAGCCCAACTTTTTAAGCCAAGCTTGGAAGCTCCCGCTCTCTTCCAAG 158 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 193000 CTAATTAAGTCTTAGCCCAACTTTTTAAGCCAAGCTTGGAAGCTCCCGCTCTCTTCCAAG 19305 *F 9 *F Query: 157 CTTTTTTCGCGCTCTTTGTGGAGCGCGGCTTTTGGTGGCTCGCCTGGCAATGCAATTCAG 98 *F |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| *F Sbjct: 193060 CTTTTTTCGCGCTCTTTGTGGAGCGCGGCTTTTGGTAGCTCGCCTGGCAATGCAATTCAG 19311 *F 9 *F Query: 97 TTGCCAAACCAAACGCGATCGGCTAACAACCTCGTACCGCTCGCTCGCAGCTTTAGCTTT 38 *F ||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 193120 TTGCCAAACAAAACGCGATCGGCTAACAACCTCGTACCGCTCGCTCGCAGCTTTAGCTTT 19317 *F 9 *F Query: 37 ACCGTTACGCCAGTGATTGAGCACTACTATATACCA 2 *F |||||||| |||||||||||||||||| |||| | | *F Sbjct: 193180 ACCGTTACACCAGTGATTGAGCACTACAATATCCGA 193215 *F >l(2)07837=? *F p_name l(2)07837 *F name CG3245 *F gene PpN58A *F ct_name CT10905 *F relation behind *F r_orientation \+ *F inside_intron 0 *F inside_exon 0 *F dist5 5491 *F dist3 6551 *F [l(2)07837 5.5kb upstream of PpN58A, in same orientation, but not near any *F other gene] *F >l(2)08014=? *F [no computed analysis for l(2)08014 but BLASTN of All indicates insertion in *F positive orientation at 186016 of AE003626.1; CG4539 starts at 186414 so *F ~400bp upstream in same orientation as gene] *F gadfly|SEG:AE003626|gb|AE003626.1|Drosophila melanogaster genomic scaffold *F 142000013386055 section 19 of 63, complete sequence.|AE003626.1 *F GI:7297545 *F Length = 265,236 *F Plus Strand HSPs: *F Score = 1502 (225.4 bits), Expect = 3.2e-61, P = 3.2e-61 *F Identities = 330/354 (93%), Positives = 330/354 (93%), Strand = Plus / Plus *F Query: 1 GCGCCAAGTCGAGAATTTGTTCCTTCAGTCTCGTCATCTGTTTCATCATTTGAGGCATAA 60 *F GCGCCAAGTCGAGAATTTGTTCCTTCAGTCTCGTCATCTGTTTCATCATTTGAGGCATAA *F Sbjct: 185960 GCGCCAAGTCGAGAATTTGTTCCTTCAGTCTCGTCATCTGTTTCATCATTTGAGGCATAA 18601 *F 9 *F Query: 61 CACTCTTCCATTTAAGCACTTTAATTCACTTGATGTAAAACTATTTACGAAGAGCACACA 120 *F CACTCTTCCATTTAAGCACTTTAATTCACTTGAT TAAAACTATTTACGAAGAGCACACA *F Sbjct: 186020 CACTCTTCCATTTAAGCACTTTAATTCACTTGATATAAAACTATTTACGAAGAGCACACA 18607 *F 9 *F Query: 121 ACTTACCATTTCGACTCTTGAAATAAAATGAACGAGTTTCGTCATTGCACGTGGAAGGAA 180 *F ACTTACCATTTCGA TCTTGAAATAAAATGAACGAGTTTCGTCAT GCACGTGGAAGGAA *F Sbjct: 186080 ACTTACCATTTCGAGTCTTGAAATAAAATGAACGAGTTTCGTCATCGCACGTGGAAGGAA 18613 *F 9 *F Query: 181 AAATTCTGTGAAAAGATGGCGGCCAACTATCGATGTCTCTGAATGCAACCATGGTAGTAT 240 *F AAATTCTGTGAAAAGATGGCGGCCAACTATCGATGTCTCTGAATGCAACCATGGTAGTAT *F Sbjct: 186140 AAATTCTGTGAAAAGATGGCGGCCAACTATCGATGTCTCTGAATGCAACCATGGTAGTAT 18619 *F 9 *F Query: 241 CGTATCGGAAAAATTGTTTGGTTTTTGCCTGATATATATTGTATAAGAATGTG-AATAAA 299 *F CGTATCGGAAAAATTGTTTGGTTTTTGCCTGATATATATTGTATAAGAATGTG AATAA *F Sbjct: 186200 CGTATCGGAAAAATTGTTTGGTTTTTGCCTGATATATATTGTATAAGAATGTGCAATAAT 18625 *F 9 *F Query: 300 TGATATT-CC-ATTTAAT--T-TTC-ATTAAATCATTCATAAAATACTTAATATA 348 *F T A T CC A T AAT T TTC ATT AAT TTCAT AAAT TT ATA A *F Sbjct: 186260 TAACTTAACCGAATAAATGATATTCCATTTAATT-TTCATTAAATCATTCATAAA 186313 *F >l(2)08307 line has insertion that maps to CG3971 (on III) *F p_name l(2)08307 *F name CG3971 *F gene CG3971 *F ct_name CT13185 *F relation inside *F r_orientation \+ *F inside_intron 1 *F inside_exon 0 *F dist5 296 *F dist3 11219 *F [l(2)08307 inserted in same orientation as CG3971 in first intron about 120bp *F downstream of end of first exon; element maps to 33A but gene maps to 73B; *F multiple insert strain or needs to be resequenced?; l(3)02281 and l(3)neo21 *F also appear to have insertions in this intron, as well as a number of 3rd *F chromosome EP lines] *F >l(2)08492=CG1952 *F p_name l(2)08492 *F name CG1952 *F gene CG1952 *F ct_name CT6120 *F relation inside *F r_orientation \+ *F inside_intron 0 *F inside_exon 2 *F dist5 685 *F dist3 3063 *F [l(2)08492 in second exon of CG1952, in same orientation as gene] *F >l(2)08717=CG15095 *F p_name l(2)08717 *F name CG15095 *F gene CG15095 *F ct_name CT34970 *F relation inside *F r_orientation \- *F inside_intron 1 *F inside_exon 0 *F dist5 1790 *F dist3 3335 *F [l(2)08717 in opposite orientation of gene in first long intron about 1.3kb *F downstream of end of first exon] # *U FBrf0132132 *a Levis *b R.W. *t 2000.11.20 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Mon Nov 20 22:47:56 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 20 Nov 2000 22:47:56 \+0000 *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Mon, 20 Nov 2000 17:48:20 \-0500 *F To: Gillian Millburn (Genetics) *F From: Robert Levis *F Subject: Re: Unannotated gap in genomic sequence and error in cn gene *F sequence *F Gillian, *F . *F . *F In the course of mapping the flanking sequence of a P insertion line *F on the genomic sequence, I found evidence for a 1.8 kb deletion *F disrupting the cinnabar (cn) gene in the BDGP/Celera sequence, *F relative to a previously published sequence of the same region *F (GenBank accession U56245). When I asked Dr. Susan Celniker of the *F BDGP about this discrepancy, she replied that she is confident that *F the BDGP/Celera sequence in this region is correct. She noted that *F the genomic DNA sequenced by BDGP/Celera was from a strain with a *F mutant cn allele (genotype y; cn bw sp). One may therefore infer *F that this deletion is responsible for the mutant cn phenotype. *F Here are the details of the putative cn1 lesion. U56245 is a 12.3 *F kb genomic sequence that includes the cn gene from a strain with a *F wildtype cn allele. The U56245 sequence partially overlaps the *F BDGP/Celera scaffold segment AE003839. A dot plot of U56245 (reverse *F complement) (Y axis) vs AE003839 nts 1-10000 (X-axis) (see attached *F Picture File of the output window) shows a deletion of ~1.8 kb in *F AE003839 between nucleotides 1863 and 1864. I also did a BLAST of *F the entire AE003839 vs U56245 (see attached html file of the output). *F The deletion occurs at position 457 of the cn gene predicted amino *F acid sequence. The deletion fuses the truncated cn ORF to an *F unrelated ORF. The cn protein predicted by the wild-type U56245 *F sequence (GenPept locus AAC47351) is 524 aa, while that predicted by *F the cn1 allele of the AE003839 sequence (GenPept locus AAF59196) is *F 504 aa. These two predicted proteins are nearly identical from 1-457 *F but then have no significant similarity beyond this point. *F Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210 *F phone: (410) 554-1238 *F fax: (410) 467-1147 *F email: levis@ciwemb.edu *F \------------------------------------ *F [FlyBase curator comment: attached Picture File and html file are archived.] # *U FBrf0132133 *a Butler *b H. *t 2000.11.13 *T personal communication to FlyBase *u *F From hb246@gen.cam.ac.uk Mon Nov 13 14:07:05 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 13 Nov 2000 14:07:05 \+0000 *F To: hb246@gen.cam.ac.uk, gm119@gen.cam.ac.uk *F Subject: Re: newgenes *F X-Sun-Charset: US-ASCII *F From: Heather Butler *F Date: Mon, 13 Nov 2000 14:08:16 \+0000 *F Content-Length: 12687 *F Hello, *F All can be matched to CGs (and their translations match too...at least *F to degree (I checked on exon of each)). *F Lovely! *F H *F \*a AC014407 *F \*E FBrf0128813 == gm8389.h == Liu and Kipreos, 2000, Molec. Biol. Evol. *F 17(7): 1061--1074 *F Note: *F This gene is a predicted cyclin-dependent kinase. They give the coordinates *F of the gene relative to AC014407 (Celera draft sequence): exons are *F at 22352-22122, 22098-21714, 21651-21501 and 21448-21277. They refer *F to the gene throughout the text as 'AC014407'. gm000821. *F > *F \*a CG6800 *F \*E FBrf0128813 == gm8389.h == hb001113.e == Liu and Kipreos, 2000, Molec. *F Biol. Evol. 17(7): 1061--1074 *F \*F cyclin-dependent protein kinase ; GO:0004693 | inferred from sequence *F similarity *F \*d protein phosphorylation ; GO:0006468 | inferred from sequence similarity *F 21448-21277 *F \----------- *F >CG6800|FBgn0038902|CT21089|FBan0006800 last_updated:000321 *F Length = 909 *F Minus Strand HSPs: *F Score = 860 (129.0 bits), Expect = 2.5e-33, P = 2.5e-33 *F Identities = 172/172 (100%), Positives = 172/172 (100%), Strand = Minus / Plus *F Query: 172 GTTTCCCAACTCCGTGGGCATTCACTGGGACAACTTGTTTCCCAGCTGCACACATGCGGT 113 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 738 GTTTCCCAACTCCGTGGGCATTCACTGGGACAACTTGTTTCCCAGCTGCACACATGCGGT 797 *F Query: 112 GGAAATCAATCTGGTCTCGAATCTGGTGGTCTACAATCCCAAAAACCGACTCAAGGCCAG 53 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 798 GGAAATCAATCTGGTCTCGAATCTGGTGGTCTACAATCCCAAAAACCGACTCAAGGCCAG 857 *F Query: 52 CGAGGTGGGTTCTGCTTCAAGTTTGACACCGGTTAGGTATTATAATTGGTAA 1 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 858 CGAGGTGGGTTCTGCTTCAAGTTTGACACCGGTTAGGTATTATAATTGGTAA 909 *F 22352-22122 *F \----------- *F >CG6800|FBgn0038902|CT21089|FBan0006800 last_updated:000321 *F Length = 909 *F Minus Strand HSPs: *F Score = 1021 (153.2 bits), Expect = 1.3e-40, P = 1.3e-40 *F Identities = 215/227 (94%), Positives = 215/227 (94%), Strand = Minus / Plus *F Query: 231 ATGGAGGACTATGCGCCCAGCCGCTATAAAATGCTTGAGAAAATCGGCGAAGGCGTCCAT 172 *F ATGGAGGACTATGCGCCCAGCCGCTATAAAATGCTTGAGAAAATCGGCGAAGGCGTCCAT *F Sbjct: 1 ATGGAGGACTATGCGCCCAGCCGCTATAAAATGCTTGAGAAAATCGGCGAAGGCGTCCAT 60 *F Query: 171 GGCTGCGTGTTTAAGGCCATCGATCTGCAGCGCAACAAGGAGGTGGCCATCAAGAAGGTG 112 *F GGCTGCGTGTTTAAGGCCATCGATCTGCAGCGCAACAAGGAGGTGGCCATCAAGAAGGTG *F Sbjct: 61 GGCTGCGTGTTTAAGGCCATCGATCTGCAGCGCAACAAGGAGGTGGCCATCAAGAAGGTG 120 *F Query: 111 GCCCTTAAGAACAAGTTCGGAAACATAGCCCTAAATACTTTGAGGGAAATCAAGACCCTG 52 *F GCCCTTAAGAACAAGTTCGGAAACATAGCCCTAAATACTTTGAGGGAAATCAAGACCCTG *F Sbjct: 121 GCCCTTAAGAACAAGTTCGGAAACATAGCCCTAAATACTTTGAGGGAAATCAAGACCCTG 180 *F Query: 51 CAGCTTTGCAAGTCTGAATATGTAG-GTATACCATATATCTATTTTC 6 *F CAGCTTTGCAAGTCTGAATAT T G A A CAT AT TAT T C *F Sbjct: 181 CAGCTTTGCAAGTCTGAATATATTCTGGACATCATTGATATATATCC 227 *F \# *F \*a AC017581 *F \*E FBrf0128813 == gm8389.h == Liu and Kipreos, 2000, Molec. Biol. Evol. *F 17(7): 1061--1074 *F Note: *F This gene is a predicted cyclin-dependent kinase. They give the coordinates *F of the gene relative to AC017581 (Celera draft sequence): exons are *F at 96285-96040, 95976-95588 and 95296-94819. They refer to the gene *F throughout the text as 'AC017581'. gm000821. *F > *F \*a CG7597 *F \*E FBrf0128813 == gm8389.h == hb001113.e == Liu and Kipreos, 2000, Molec. *F Biol. Evol. 17(7): 1061--1074 *F \*F cyclin-dependent protein kinase ; GO:0004693 | inferred from sequence *F similarity *F \*d protein phosphorylation ; GO:0006468 | inferred from sequence similarity *F 95296-94819 *F \----------- *F >CG7597|FBgn0037093|CT23101|FBan0007597 last_updated:000321 *F Length = 3644 *F Minus Strand HSPs: *F Score = 2370 (355.6 bits), Expect = 3.9e-102, P = 3.9e-102 *F Identities = 474/474 (100%), Positives = 474/474 (100%), Strand = Minus / Plus *F Query: 478 GGGTAAAGTAAAACTAGCTGACTTTGGGCTGGCACGACTATACAATGCGGACGATCGGGA 419 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3011 GGGTAAAGTAAAACTAGCTGACTTTGGGCTGGCACGACTATACAATGCGGACGATCGGGA 3070 *F Query: 418 ACGCCCCTACACGAACAAAGTAATTACCTTGTGGTATCGTCCTCCTGAGCTTTTGCTGGG 359 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3071 ACGCCCCTACACGAACAAAGTAATTACCTTGTGGTATCGTCCTCCTGAGCTTTTGCTGGG 3130 *F Query: 358 TGAGGAGCGTTACGGCCCGTCGATAGACGTGTGGTCCTGCGGGTGCATCCTCGGCGAACT 299 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3131 TGAGGAGCGTTACGGCCCGTCGATAGACGTGTGGTCCTGCGGGTGCATCCTCGGCGAACT 3190 *F Query: 298 CTTTGTGAAGCGGCCCCTGTTCCAGGCTAATGCGGAAATGGCGCAGCTTGAAACGATATC 239 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3191 CTTTGTGAAGCGGCCCCTGTTCCAGGCTAATGCGGAAATGGCGCAGCTTGAAACGATATC 3250 *F Query: 238 AAAGATCTGTGGATCCCCGGTTCCTGCCGTGTGGCCGAATGTAATTAAGCTGCCTCTGTT 179 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3251 AAAGATCTGTGGATCCCCGGTTCCTGCCGTGTGGCCGAATGTAATTAAGCTGCCTCTGTT 3310 *F Query: 178 CCACACTCTCAAACAAAAGAAGACGCACCGCCGCCGTCTACGAGAAGACTTTGAATTCAT 119 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3311 CCACACTCTCAAACAAAAGAAGACGCACCGCCGCCGTCTACGAGAAGACTTTGAATTCAT 3370 *F Query: 118 GCCAGCCCCTGCACTTGATCTGCTGGACAAAATGCTTGATTTAGATCCGGACAAGCGCAT 59 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3371 GCCAGCCCCTGCACTTGATCTGCTGGACAAAATGCTTGATTTAGATCCGGACAAGCGCAT 3430 *F Query: 58 CACAGCAGAAGACGCGCTCCGGTCACCGTGGCTAAGGAAAATCAACCCCGACGA 5 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3431 CACAGCAGAAGACGCGCTCCGGTCACCGTGGCTAAGGAAAATCAACCCCGACGA 3484 *F 95976-95588 *F \----------- *F >CG7597|FBgn0037093|CT23101|FBan0007597 last_updated:000321 *F Length = 3644 *F Minus Strand HSPs: *F Score = 1945 (291.8 bits), Expect = 2.5e-82, P = 2.5e-82 *F Identities = 389/389 (100%), Positives = 389/389 (100%), Strand = Minus / Plus *F Query: 389 GTTTACAAGGCGCGGGATCATCATACCAATGACATGGTAGCACTGAAGAAGGTTCGGCTG 330 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2622 GTTTACAAGGCGCGGGATCATCATACCAATGACATGGTAGCACTGAAGAAGGTTCGGCTG 2681 *F Query: 329 GAGCACGAGAAGGAGGGCTTTCCCATTACAGCTGTTCGTGAAATCAAAATTCTGAGACAA 270 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2682 GAGCACGAGAAGGAGGGCTTTCCCATTACAGCTGTTCGTGAAATCAAAATTCTGAGACAA 2741 *F Query: 269 CTTAACCATCGCAATATAGTCAACCTGCATGAAATTGTGACGGACAAGCAGGATGCTGTG 210 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2742 CTTAACCATCGCAATATAGTCAACCTGCATGAAATTGTGACGGACAAGCAGGATGCTGTG 2801 *F Query: 209 GAGTTCCGTAAAGACAAGGGATCCTTCTACTTGGTGTTTGAGTACATGGATCACGACTTG 150 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2802 GAGTTCCGTAAAGACAAGGGATCCTTCTACTTGGTGTTTGAGTACATGGATCACGACTTG 2861 *F Query: 149 ATGGGTCTGCTGGAGTCTGGCATGGTCGACTTCAACGAGGAAAACAACGCAAGCATTATG 90 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2862 ATGGGTCTGCTGGAGTCTGGCATGGTCGACTTCAACGAGGAAAACAACGCAAGCATTATG 2921 *F Query: 89 AAGCAGCTCTTGGACGGCCTCAACTACTGCCACAAAAAGAATTTTCTGCATCGAGACATC 30 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2922 AAGCAGCTCTTGGACGGCCTCAACTACTGCCACAAAAAGAATTTTCTGCATCGAGACATC 2981 *F Query: 29 AAGTGCTCGAACATTTTAATGAACAACAG 1 *F ||||||||||||||||||||||||||||| *F Sbjct: 2982 AAGTGCTCGAACATTTTAATGAACAACAG 3010 *F \# *F \*a AC018104 *F \*E FBrf0128813 == gm8389.h == Liu and Kipreos, 2000, Molec. Biol. Evol. *F 17(7): 1061--1074 *F Note: *F This gene is a predicted cyclin-dependent kinase. They give the coordinates *F of the gene relative to AC018104 (Celera draft sequence): exons are *F at 100085-99918 and 99860-98946. They refer to the gene throughout *F the text as 'AC018104'. gm000821. *F > *F \*a CG7236 *F \*E FBrf0128813 == gm8389.h == hb001113.e == Liu and Kipreos, 2000, Molec. *F Biol. Evol. 17(7): 1061--1074 *F \*F cyclin-dependent protein kinase ; GO:0004693 | inferred from sequence *F similarity *F \*d protein phosphorylation ; GO:0006468 | inferred from sequence similarity *F 99860-98946 *F \----------- *F >CG7236|FBgn0031730|CT22313|FBan0007236 last_updated:000321 *F Length = 1179 *F Minus Strand HSPs: *F Score = 4365 (654.9 bits), Expect = 9.5e-192, P = 9.5e-192 *F Identities = 873/873 (100%), Positives = 873/873 (100%), Strand = Minus / Plus *F Query: 915 AACCTGAAGCATCCGAACCTTGTCTCCCTGCTAGAAGTGTTCCGACGGAAGCGACGCCTC 856 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 169 AACCTGAAGCATCCGAACCTTGTCTCCCTGCTAGAAGTGTTCCGACGGAAGCGACGCCTC 228 *F Query: 855 CATCTGGTCTTCGAGTTCTGCGAGCTGACCGTGCTGCACGAACTGGAGCGGCATCCACAG 796 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 229 CATCTGGTCTTCGAGTTCTGCGAGCTGACCGTGCTGCACGAACTGGAGCGGCATCCACAG 288 *F Query: 795 GGCTGCCCGGAGCACCTGACCAAACAGATCTGCTACCAGACCCTGCTGGGCGTGGCCTAC 736 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 289 GGCTGCCCGGAGCACCTGACCAAACAGATCTGCTACCAGACCCTGCTGGGCGTGGCCTAC 348 *F Query: 735 TGCCACAAGCAGGGCTGCCTACACCGCGACATCAAGCCGGAGAACATTCTGCTGACGGCC 676 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 349 TGCCACAAGCAGGGCTGCCTACACCGCGACATCAAGCCGGAGAACATTCTGCTGACGGCC 408 *F Query: 675 CAGGGTCAGGTGAAGCTGTGTGACTTCGGCTTCGCCCGGATGCTCAGTCCGGGCGAGAAT 616 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 409 CAGGGTCAGGTGAAGCTGTGTGACTTCGGCTTCGCCCGGATGCTCAGTCCGGGCGAGAAT 468 *F Query: 615 TACACGGACTACGTGGCCACCAGATGGTACCGGGCGCCGGAGCTGCTGGTGGGCGACACT 556 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 469 TACACGGACTACGTGGCCACCAGATGGTACCGGGCGCCGGAGCTGCTGGTGGGCGACACT 528 *F Query: 555 CAGTACGGCACTCCGGTGGACGTCTGGGCCATCGGTTGCCTCTTCGCCGAGCTGGTCCGG 496 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 529 CAGTACGGCACTCCGGTGGACGTCTGGGCCATCGGTTGCCTCTTCGCCGAGCTGGTCCGG 588 *F Query: 495 GGCGAGGCCCTCTGGCCAGGACGTAGCGATGTGGATCAGCTCTATCTGATCCGCAAGACG 436 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 589 GGCGAGGCCCTCTGGCCAGGACGTAGCGATGTGGATCAGCTCTATCTGATCCGCAAGACG 648 *F Query: 435 CTCGGCGACCTGCTGCCGCGCCACATCCAGATCTTTGGACAGAACGAGTACTTTAAGGGC 376 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 649 CTCGGCGACCTGCTGCCGCGCCACATCCAGATCTTTGGACAGAACGAGTACTTTAAGGGC 708 *F Query: 375 ATCACGCTGCCAGTGCCGCCGACGCTGGAGCCGCTGGAGGACAAGATGCCGGCCAAGTCG 316 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 709 ATCACGCTGCCAGTGCCGCCGACGCTGGAGCCGCTGGAGGACAAGATGCCGGCCAAGTCG 768 *F Query: 315 CAGCAGAATCCCCTGACCATTGACTTTCTCAAGAAGTGCCTGGACAAGGATCCGACCAAG 256 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 769 CAGCAGAATCCCCTGACCATTGACTTTCTCAAGAAGTGCCTGGACAAGGATCCGACCAAG 828 *F Query: 255 CGCTGGTCCTGCGAGAAGCTCACAAAGCACTCCTACTTCGATGACTACATCGCTAAGCAG 196 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 829 CGCTGGTCCTGCGAGAAGCTCACAAAGCACTCCTACTTCGATGACTACATCGCTAAGCAG 888 *F Query: 195 CGCGAACTGGAGCACGTCAACAGCCTGGAGGCGGCCAATCTCCGCCAGCAGCAGCTCGCC 136 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 889 CGCGAACTGGAGCACGTCAACAGCCTGGAGGCGGCCAATCTCCGCCAGCAGCAGCTCGCC 948 *F Query: 135 TCCCAGCAGTTCATGCTGGCCACGGCGGCCCAGCAGCTCCAGACGGGTCCTGCTCAGGCG 76 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 949 TCCCAGCAGTTCATGCTGGCCACGGCGGCCCAGCAGCTCCAGACGGGTCCTGCTCAGGCG 1008 *F Query: 75 GCGGCCATCGCGGCGGCCCGGGATAAATCAAAG 43 *F ||||||||||||||||||||||||||||||||| *F Sbjct: 1009 GCGGCCATCGCGGCGGCCCGGGATAAATCAAAG 1041 *F 100085-99918 *F \------------ *F >CG7236|FBgn0031730|CT22313|FBan0007236 last_updated:000321 *F Length = 1179 *F Minus Strand HSPs: *F Score = 835 (125.3 bits), Expect = 2.6e-32, P = 2.6e-32 *F Identities = 167/167 (100%), Positives = 167/167 (100%), Strand = Minus / Plus *F Query: 167 TGGATCGCTATGAGAAGCTCAGTCGGCTGGGCGAGGGCTCCTACGGTGTGGTCTACAAGT 108 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2 TGGATCGCTATGAGAAGCTCAGTCGGCTGGGCGAGGGCTCCTACGGTGTGGTCTACAAGT 61 *F Query: 107 GCCGGGATCGGGAAACGGGTGCTCTGGTGGCGGTCAAGAGGTTTGTGGAGTCCGAGGATG 48 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 62 GCCGGGATCGGGAAACGGGTGCTCTGGTGGCGGTCAAGAGGTTTGTGGAGTCCGAGGATG 121 *F Query: 47 ATCCAGCGATTCGTAAAATTGCACTGCGAGAAATTAGGCTACTGAAG 1 *F ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 122 ATCCAGCGATTCGTAAAATTGCACTGCGAGAAATTAGGCTACTGAAG 168 # *U FBrf0132134 *a Whitfield *b E. *t 2000.11.21 *T personal communication to FlyBase *u FlyBase error report for CG5817 on Tue Nov 21 06:15:53 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Nov 21 14:16:09 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 21 Nov 2000 14:16:09 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 21 Nov 2000 06:15:53 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5817 on Tue Nov 21 06:15:53 2000 *F Content-Length: 697 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5817 *F Release: 1 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG5817. *F Comments: BG:DS09218.1 has been deleted from Celera release 2. It was *F previously present *F as an N-terminal fragment in release 1. *F Could you please include a CDS feature for this translation, unless you have *F other reasons to believe this translation should not be reinstated. *F The entire translation is present and 100% identical to that from Ashburner et *F al, Genetics 153:179-219(1999) (AE003416; AAF45010). *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132135 *a Whitfield *b E. *t 2000.10.30 *T personal communication to FlyBase *u FlyBase error report on Mon Oct 30 16:31:09 2000. *F From eleanor@ebi.ac.uk Mon Oct 30 16:26:36 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 30 Oct 2000 16:26:36 \+0000 *F Date: Mon, 30 Oct 2000 16:31:09 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.06 en (Win95; I) *F MIME-Version: 1.0 *F To: 'Gillian Millburn (Genetics)' *F CC: Eleanor Whitfield *F Subject: Re: list of potentially CGable genes *F Content-Transfer-Encoding: 7bit *F Gillian, *F A few hits for you, though not many \- sorry! *F a quick hour turned into half a day, I should know better by now! It took *F so long as I confirmed the alignments by updating the entries which is what *F took the time. *F nc1: *F CdsA = CG7962, 1 aa difference *F Dup99B \- no match to Celera *F Hsp70Aa \- only 2aa fragment, not enough for a unique match *F Hsp70Ba \- only 12aa fragment, not enough for a unique match *F Hsp70Bc \- only 11aa fragment, not enough for a unique match *F Nems \- no match to Celera *F nc2: *F Pk1 \- no match to Celera *F nc3: *F BobA = CG13465, 100% identical protein *F BobB = CG12487, 2aa difference *F BobC = *F BobA, B and C have identical protein translations *F Celera only has 2 genes (I really cannot find a third) and I cannot map *F which CG to which Bob *F order of increasing cytology CG13465- CG12487- *F BobA = CG12487 but not CG13465 *F BobB = CG13465 or CG12487 *F BobC = CG13465 but not CG12487 *F For the moment I have arbitrarily mapped BobA=CG12487, BobB=CG13465 *F EG:BACH7M4.2 \- no match to Celera *F Kisir \- CG9131, 100% identical protein *F mei-217 \- no match to Celera *F . *F . *F . *F Ele # *U FBrf0132145 *a Basler *b K. *t 2000.11.30 *T personal communication to FlyBase *u *F >From gelbart@morgan.harvard.edu Fri Sep 29 09:47 EDT 2000 *F >From: William Gelbart *F >Date: Fri, 29 Sep 2000 09:45:51 \-0400 (EDT) *F >To: basler@molbio.unizh.ch *F >Subject: help with type II receptor entries *F >Cc: gelbart@morgan.harvard.edu *F > *F > *F >Dear Konrad, *F > *F >So far as I can tell, the two FlyBase genes wit and Stk-D *F >are likely to refer to the same type II receptor gene. In *F >both cases, the existence of these genes is attributed to *F >papers or GenBank entries from your lab. *F > *F >If they are the same, I assume that you would prefer that *F >the Stk-D entry be withdrawn in favor of wit. *F > *F >If you could send me a note resolving the relationship of *F >these entries that I could send on as a personal communication *F >to FlyBase, I would appreciate it. *F > *F >Thanks and best wishes, *F > *F >Bill *F >From basler@molbio.unizh.ch Thu Nov 30 16:39 EST 2000 *F Mime-Version: 1.0 *F X-Sender: basler@mailhost.unizh.ch *F Date: Thu, 30 Nov 2000 22:39:01 \+0100 *F To: William Gelbart *F From: Konrad Basler *F Subject: Re: help with type II receptor entries *F Dear Bill, *F . *F . *F Yes, indeed the wit sequence *F I found on the web matches the STK-D sequence. I agree that it would *F be best to remove STK-D from Flybase. *F Best wishes! *F Konrad # *U FBrf0132146 *a O'Connor *b M.B. *t 2000.12.5 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Mon Dec 04 11:25:46 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 4 Dec 2000 11:25:46 \+0000 *F To: moconnor@gene.med.umn.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 4 Dec 2000 11:25:46 \+0000 *F Content-Length: 2669 *F Dear Dr. O'Connor, *F I am curating your paper for FlyBase: *F Chavez et al., 2000, Development 127(19): 4115--4126 *F . *F . *F . *F we have a record of a gene from your ADRC abstract: *F Marques et al., 1996, A. Dros. Res. Conf. 37: 195 *F 'New Drosophila receptor of the TGF-beta superfamily: the plot *F thickens.' *F In this abstract you called the gene 'SE20' and say that it is a new *F type II TGF beta receptor. *F I suspect that this may be the same gene as 'wit' (wishful thinking) *F given that one of the symbols of the rescue constructs used in your *F Development paper is 'SE20'. I would be grateful if you could confirm *F whether or not 'SE20' from your abstract is the same as 'wit' (if it is *F I will merge the two gene entries in FlyBase, keeping the valid symbol *F as 'wit'), *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From moconnor@lenti.med.umn.edu Tue Dec 05 16:20:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 5 Dec 2000 16:20:53 \+0000 *F Mime-Version: 1.0 *F Date: Tue, 5 Dec 2000 10:12:27 \-0800 *F To: Gillian Millburn (Genetics) *F From: 'Michael B. O'Connor' *F Subject: Re: FlyBase query *F Dear Gillian, *F . *F . *F . *F > *F >we have a record of a gene from your ADRC abstract: *F > *F >Marques et al., 1996, A. Dros. Res. Conf. 37: 195 *F > *F >'New Drosophila receptor of the TGF-beta superfamily: the plot *F >thickens.' *F > *F >In this abstract you called the gene 'SE20' and say that it is a new *F >type II TGF beta receptor. *F Yes wit = SE20. *F > *F sincerely, *F Mike O'Connor *F Michael B. O'Connor *F HHMI *F Department of Genetics, Cell Biology, and Development *F University of Minnesota *F Room 6-160 Jackson Hall, *F 321 Church Street SE *F Minneapolis, MN *F 55455 *F tel. 612-626-0642 *F fax 612-625-5402 *F moconnor@mail.med.umn.edu *F http://www.gcd.med.umn.edu/OConnor/ # *U FBrf0132151 *a Whitfield *b E. *t 2000.11.22 *T personal communication to FlyBase *u FlyBase error report for CG10714 on Wed Nov 22 02:58:45 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Nov 22 10:58:49 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 22 Nov 2000 10:58:49 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 22 Nov 2000 02:58:45 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10714 on Wed Nov 22 02:58:45 2000 *F Content-Length: 511 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10714 *F Release: 1 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG10714. *F Comments: This CG has been deleted from release 2 and is corresponds to Ly *F (FBgn0002573). *F Could it please be reinstated? *F (there is no internal note in genes.nice to state this CG was deleted so I was *F unsure of its status) *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132152 *a Whitfield *b E. *t 2000.11.24 *T personal communication to FlyBase *u FlyBase error report for CG9204 on Fri Nov 24 03:32:31 2000. *F From FlyBase-error@hedgehog.lbl.gov Fri Nov 24 11:32:45 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 24 Nov 2000 11:32:45 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 24 Nov 2000 03:32:31 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9204 on Fri Nov 24 03:32:31 2000 *F Content-Length: 950 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9204 *F Release: 1 *F Gene annotation error *F Gene CG9204 has incorrect exon/intron structure. *F Comments: Celera have annotated 3 variant for Ate1: *F AE003793; AAF57496 *F AE003793; AAF57497 *F AE003793; AAF57498 *F AAF57496 is the correct one, the other tow have incorrect splice sites. *F AAF57497 has an incorrect splice site at the beginning of exon 3, it is in *F frame and encodes for an extra 7 amino acids. *F AAF57498 has a wrong splice site at the end of exon 2, encodes a unique exon 3 *F but the remainder of the exons are out of phase suggesting the unique exon is *F wrong. *F Also, Kwon et al, Molec. Cell. Biol. 19(1):182-193(1999) failed to find an *F alternative exon in Drosophila that exists in human and mouse, suggesting that *F Drosophila does not have alternative splicing for this protein. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132153 *a Whitfield *b E. *t 2000.11.28 *T personal communication to FlyBase *u FlyBase error report for CG3903 on Tue Nov 28 02:23:39 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Nov 28 10:23:52 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Nov 2000 10:23:52 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Nov 2000 02:23:39 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3903 on Tue Nov 28 02:23:39 2000 *F Content-Length: 529 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3903 *F Release: 1 *F Gene annotation error *F Gene CG3903 has incorrect exon/intron structure. *F Comments: Celera annotation of Gli (AE003648; AAF53482) is missing the *F N-terminal exon. *F Please amend CDS and mRNA features. *F Please compare to Auld et al, Cell 81:757-767(1995) (L39083; AAC41579) and *F Ashburner et al, Genetics 153:179-219(1999) (AE003414; AAF44964) *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132154 *a Whitfield *b E. *t 2000.11.28 *T personal communication to FlyBase *u FlyBase error report for CG5868 on Tue Nov 28 03:28:12 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Nov 28 11:28:37 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Nov 2000 11:28:37 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Nov 2000 03:28:12 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5868 on Tue Nov 28 03:28:12 2000 *F Content-Length: 643 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5868 *F Release: 1 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG5868. *F Comments: In release 1 Celera had the correct 2 splice variants for Psn *F (agreeing 100% *F with published data and the gene was called Psn \- perfect, wonderful! *F In release 2 Celera has not annotated the shorter isoform and has named the *F remaining isoform CG18803, instead of Psn \- not so good. *F Could release 3 please be augmented to reflect release 1 data?! *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132155 *a Salz *b H. *t 2000.11.28 *T personal communication to FlyBase *u FlyBase error report for CG1341 on Tue Nov 28 11:13:43 2000. *F From FlyBase-error@hedgehog.lbl.gov Tue Nov 28 19:13:53 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 28 Nov 2000 19:13:53 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Nov 2000 11:13:44 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hks@po.cwru.edu *F Subject: FlyBase error report for CG1341 on Tue Nov 28 11:13:43 2000 *F Content-Length: 359 *F Error report from Helen Salz (hks@po.cwru.edu) *F Gene or accession: CG1341 *F Release: 1 *F cDNA or EST error *F Comments: CG1341 appears to be hit by P element insertions in l(2)05643 AND *F these P elements fail to complement EP(2)2153 and the EMS induced allele *F l(2)43Ed *F Browser: Mozilla/3.04Gold (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132156 *a Whitfield *b E. *t 2000.11.29 *T personal communication to FlyBase *u FlyBase error report for CG1634 on Wed Nov 29 04:39:25 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Nov 29 12:39:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 29 Nov 2000 12:39:43 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 29 Nov 2000 04:39:26 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1634 on Wed Nov 29 04:39:25 2000 *F Content-Length: 653 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1634 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG1634. *F Comments: A second longer isoform of Nrg exists, please add a CDS feature for *F it. *F Isoforms are discussed in Hortsch, Neuron 4:697-709(1990) (X76243; CAA53822 *F and X76244; CAA53823 for the alternative C terminal exons). *F A more recent paper is Zhao and Hortsch, Gene 215:47-55(1998) giving full length *F submissions of the splice forms (AF050085; AAC28613 and AF050085; AAC28614). *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0132157 *a Freeman *b M. *t 2000.11.29 *T personal communication to FlyBase *u FlyBase error report for CG4385 on Wed Nov 29 18:18:33 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Nov 30 02:18:39 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Nov 2000 02:18:39 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 29 Nov 2000 18:18:33 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: freeman@uoneuro.uoregon.edu *F Subject: FlyBase error report for CG4385 on Wed Nov 29 18:18:33 2000 *F Content-Length: 276 *F Error report from Marc Freeman (freeman@uoneuro.uoregon.edu) *F Gene or accession: CG4385 *F Release: 1 *F Missed gene *F Comments: l(2)k09538 appears to be an insertion in Star *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132158 *a Mount *b S. *t 2000.11.30 *T personal communication to FlyBase *u FlyBase error report for CG17818 on Thu Nov 30 12:26:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Nov 30 20:26:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Nov 2000 20:26:31 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 30 Nov 2000 12:26:19 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sm193@umail.umd.edu *F Subject: FlyBase error report for CG17818 on Thu Nov 30 12:26:19 2000 *F Content-Length: 587 *F Error report from Steve Mount (sm193@umail.umd.edu) *F Gene or accession: CG17818 *F Release: 1 *F cDNA or EST error *F Comments: There is a microexon (8 nt.) not annotated in AE003802.2 *F GI:10727480, but clearly present based on the sequence of AF160934. The exon *F occurs at positions 157,853 through 157,860, and has the sequence AATACACG *F (CGTGTATT on the complementary strand, which is represented by AE003802.2). *F Prediction of protein sequence AAD46874.1 requires that this microexon be *F accounted for. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132159 *a Robertson *b H.M. *t 2000.12.3 *T personal communication to FlyBase *u FlyBase error report for CG8415 on Sat Dec 2 17:18:33 2000. *F From FlyBase-error@hedgehog.lbl.gov Sun Dec 03 01:18:41 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 3 Dec 2000 01:18:41 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 2 Dec 2000 17:18:33 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG8415 on Sat Dec 2 17:18:33 2000 *F Content-Length: 461 *F Error report from Hugh M. Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG8415 *F Release: 1 *F Gene annotation error *F Gene CG8415 has incorrect exon/intron structure. *F Comments: This gene as annotated encodes the S23 ribosomal protein in it's *F C-terminus, but multiple ESTs and comparison with the human and other proteins *F shows the translation should only start at aa74. *F Browser: Mozilla/4.7 (Macintosh; U; PPC) *F Accessed from: FBupdate, /www/hedgehog_8000/htdocs # *U FBrf0132160 *a Whitfield *b E. *t 2000.12.6 *T personal communication to FlyBase *u FlyBase error report for CG5102 on Wed Dec 6 08:45:37 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Dec 06 16:46:17 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 6 Dec 2000 16:46:17 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 6 Dec 2000 08:45:37 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5102 on Wed Dec 6 08:45:37 2000 *F Content-Length: 560 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5102 *F Gene annotation error *F Gene CG5102 has incorrect exon/intron structure. *F Comments: If the CDS feature is extended from *F FT CDS 153170..155278 *F to: *F FT CDS 153146..155278 *F Celera translation of this protein would be an exact match for *F J03148; AAA28442, Caudy et al, Cell 55:1061-1067(1988) *F Y00221; CAA68368, Cronmiller et al, Genes Dev. 2:1666-1676(1988) *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0132161 *a Wicker *b C. *t 2000.12.7 *T personal communication to FlyBase *u FlyBase error report on Thu Dec 7 18:10:31 2000. *F From claude.wicker@ibaic.u-psud.fr Thu Dec 07 17:03:20 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Dec 2000 17:03:20 \+0000 *F Date: Thu, 07 Dec 2000 18:10:31 \+0100 *F From: Claude Wicker *F X-Mailer: Mozilla 4.51 fr (Win98; I) *F X-Accept-Language: fr *F MIME-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F Subject: clones fad and CG 5925 *F Content-Transfer-Encoding: 7bit *F I would like to send you a few comments about the desaturase genes in 87 *F BC: *F We have sequenced and studied the region in 87C around fad (or gene *F desat1). The gene noted CG5925 does not correspond to what we have *F obtained. In fact we have found the following open reading frame (AE *F 003696): *F CDS complement (6986..7232, 7299..7635, 7754..7939, 8009..8324) *F fad gene (desat1) encodes a delta 9 desaturase which preferentially uses *F palmitate as a substrate, leading to the synthesis of omega7 fatty *F acids. The other gene (desat2) encodes also a delta 9 desaturase but *F acts preferentially on myristate, leading to the synthesis of omega 5 *F fatty acids. *F desat1 is expressed in male and female adults of all D. melanogaster *F strains. desat2 is not expressed in Canton-S adults but is expressed *F in female adults from the Tai strain. *F In D. melanogaster cuticular pheromones consist of unsaturated *F hydrocarbons with one double bond in position 7 : 7 tricosene (T) in *F males but in position 7: 7,11 heptacosadiene (HD) in females of most *F strains and in position 5 :5,9 HD in many African populations like the *F Tai strain. We suggest, therefore, that desat2 might play a control role *F in the biosynthesis of 5,9 HD hydrocarbons in Tai females and could *F explain the dienic hydrocarbon polymorphism in D. melanogaster. *F DALLERAC R., JALLON J. M., LABEUR C., KNIPPLE D. C., ROELOFS W., *F WICKER-THOMAS C. 2000 A delta9 desaturase gene with a different *F substrate specificity is responsible for the cuticular diene hydrocarbon *F polymorphism in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA.97, *F 9449-9454. # *U FBrf0132162 *a Goldberg *b M.L. *t 2000.12.7 *T personal communication to FlyBase *u FlyBase error report for CG5981 on Thu Dec 7 10:25:36 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Dec 07 18:25:59 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 7 Dec 2000 18:25:59 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 7 Dec 2000 10:25:37 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mlg11@cornell.edu *F Subject: FlyBase error report for CG5981 on Thu Dec 7 10:25:36 2000 *F Content-Length: 1173 *F Error report from Michael L. Goldberg (mlg11@cornell.edu) *F Gene or accession: CG5981 *F Release: 1 *F Gene annotation error *F Gene CG5981 should be split. *F Comments: I believe CG5981 should be split into two genes. One gene is *F represented by ESTs LD33989 and GM04023. This gene starts at nucleotide *F 144063 of AE003612; of particular importance there seems to be a large intron *F from 145466 to 165435 of the same assembly. This gene encodes the fly homolog *F of stathmin/p18. The second gene is contained within the large intron and is *F represented by EST SD08412. It is transcribed in the same direction. The 5' *F end of this EST is at approximately 162175 of AE003612, and the 3' end (i.e., *F the 3' end of SD08412) is at roughly 164145. Splitting of the two genes is *F consistent with what I can make of the two different sets of cDNAs (each of *F which has both a 5' and a 3' sequence). Also, as it currently stands (with *F the genes connected), the fly stathmin protein is roughly 4 times larger than *F it is in other organisms. *F Please let me know if you agree with this suggested update. *F Browser: Mozilla/4.75 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8000/htdocs # *U FBrf0132163 *a Gray *b T. *t 2000.8.1 *T personal communication to FlyBase *u *F >Envelope-to: jr32@mole.bio.cam.ac.uk *F >Date: Tue, 1 Aug 2000 09:07:55 \-0400 (EDT) *F >X-Sender: tag6@pop.cwru.edu *F >To: jr32@mole.bio.cam.ac.uk *F >From: tag6@po.cwru.edu (Todd Gray) *F >Subject: molybdenum coenzyme synthase I *F > *F >Dear Dr. Roote, *F > *F >Your name has been forwarded to me as a provider of annotations for the *F >Drosophila genome. In this capacity, you may be interested to know that we *F >have recently published a paper that describes the alternative splicing of *F >the molybdenum coenzyme synthase I (MOCSI) locus in many species (see RNA *F >6:928-936). All of the species produce a bicistronic transcript containing *F >separate adjacent open reading frames for MOCS1A and MOCS1B, and all *F >species also produce mRNA splice forms that fuse the ORFs to encode a *F >single fused MOCS1A-MOCS1B protein. The present genomic annotation for the *F >Drosophila locus (AC006562.8) predicts the fused form, but not the *F >bicistronic form. If our GenBank depositions would be useful, they are *F >AF24021 for the Drosophila bicistronic splice form and AF24027 for the *F >fused splice form. *F > *F > *F >Yours sincerely, *F > *F >Todd A. Gray, Ph.D. *F >Department of Genetics *F >Case Western Reserve University *F >BRB 739 *F >2109 Adelbert Road *F >Cleveland, OH 44106-4955 *F >phone: 216-368-8830 *F >fax: 216-368-3432 # *U FBrf0132164 *a True *b J. *c A. *d Kopp *e S. *f Carroll *t 2000.10.19 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Oct 19 16:29:46 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-proPO} insertions *F From: Kevin Cook, Bloomington Stock Center *F Subject: P{UAS-proPO} insertions *F John True, Artyom Kopp and Sean Carroll will describe the construction of *F P{UAS-proPO} in detail in an upcoming publication. The following *F information accompanied P{UAS-proPO} stocks they donated to the Stock *F Center (9/00). This UAS construct in pUAST contains the cDNA for *F prophenoloxidase (proPO). Expression of proPO allows the production of *F melanin in the presence of dopamine and isopropanol. UAS-proPO is an *F alternative reporter construct to UAS-lacZ or UAS-GFP. There are three *F homozygous viable and fertile insertions, one for each major chromosomes: *F P{w+mC=UAS-proPO}X *F P{w+mC=UAS-proPO}2 *F P{w+mC=UAS-proPO}3 *F The insertions have not been mapped within each chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132165 *a Hsieh *b T.S. *t 2000.10.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Oct 29 19:30:36 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Top1+t10}5-3 insertion *F The following information accompanied stocks from Tao-Shih Hsieh, Duke *F University (9/00). P{Top1+t10}5-3 is a homozygous viable and fertile, *F second chromosome insertion. The construction of P{Top1+t10} in the *F P{CaSpeR} vector and its transformation were described in FBrf0059286. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132166 *a Bourbon *b H.M. *t 2000.10.31 *T personal communication to FlyBase *u *F From bourbon@cict.fr Tue Oct 31 15:54:08 2000 *F To: flybase-help@morgan.harvard.edu *F Subject: Flybase update *F Dear flybase keeper, *F I would like to inform you that l(3)03881 is allelic with tara. Could you *F please update flybase to incorporate this novel data. Could you also *F mention that taranis is a novel trithorax-group member (Calgaro et al., *F submitted). *F Sincerely yours. *F Henri-Marc Bourbon, phD *F Senior Scientist in D. Cribbs's laboratory *F Dr. Henri-Marc Bourbon, phD *F Centre de Biologie du Developpement *F UMR5547 du C.N.R.S. *F Universite Paul Sabatier Toulouse III *F 118 Route de Narbonne *F 31062 Toulouse *F France *F e-mail: bourbon@cict.fr *F phone: (33) 05 61 55 82 88 *F Fax: (33) 05 61 55 65 05 # *U FBrf0132167 *a Roote *b J. *t 2000.10.31 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Oct 31 19:44:04 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Region 42 complementation groups *F The following information accompanied stocks from John Roote, University of *F Cambridge (10/00). *F Complementation groups were defined in region 42 and representative alleles *F were donated to the Stock Center. These include *F l(2)42Aa1C2 *F l(2)42Ab1CC *F CyO, l(2)42Bapk81i *F l(2)42Be1DC *F l(2)42Bf1A13 *F l(2)42Bg1DA *F l(2)42Bh2CA *F l(2)42Bi1D18 *F l(2)42Ca2A3 *F l(2)42Cb1C4 *F l(2)42Cc1AB *F l(2)42Cd447 *F l(2)42Da1DE *F l(2)42Ea1BH *F Tp(2;2)pk-sple22, l(2)42Ebpk-sple-22 pkpk-sple-22 *F l(2)42Ec1DP *F l(2)42Fa1A10 *F Note that l(2)42Ebpk-sple-22 is the 42E breakpoint of Tp(2;2)pk-sple22. *F This sequence of gene names depends on l(2)6G11, l(2)5CC11 and *F l(2)4LL11 being renamed l(2)42Bb6G1, l(2)42Bc5CC1 and l(2)42Bd4LL1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132168 *a Bogart *b K. *c K. *d Cook *t 2000.11.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Nov 08 16:03:38 2000 *F To: Rachel Drysdale (Genetics) *F Subject: Isolation of Df(2R)BSC3 *F Isolation of Df(2R)BSC3 *F Kevin Bogart and Kevin Cook *F Bloomington Stock Center, Indiana University *F To provide deletion coverage of the 49AB region, we induced a new *F deficiency by P transposase-induced male recombination between *F P{PZ}Cam03909 (48F1-2) and P{lacW}k15501 (49B3-6; revised cytological *F localization estimated from mapping of P insertion to 24 nucleotides 5' of *F Sin3A mRNA (Prokopenko et al. Genetics, in press)). We outline here the *F steps used in generating the deficiency and its cytological characterization. *F First, the P{lacW}k15501 insertion was marked with bw1 sp1. We *F recovered a meiotic crossover event between the P{lacW}k15501 chromosome *F from stock \#11142 and the cn1 bw1 sp1 chromosome from stock \#4455 to *F create the stock P{lacW}k15501 unchk15501 bw1 sp1/SM6a. *F To induce the deletion, we first crossed wgSp-1/CyO; ry506 Sb1 *F P{Delta2-3}99B/TM6 (stock \#2535) females to P{lacW}k15501 unchk15501 *F bw1 sp1/SM6a males. P{lacW}k15501 unchk15501 bw1 sp1/CyO; ry506 *F Sb1 P{Delta2-3}99B/+ males were crossed to cn1 P{PZ}Cam03909/CyO; *F ry506 (stock \#11356) females to generate males of the genotype cn1 *F P{PZ}Cam03909/P{lacW}k15501 unchk15501 bw1 sp1; ry506 Sb1 *F P{Delta2-3}99B/ry506. Potential deletion-bearing (cn bw) or tandem *F duplication-bearing (cn+ bw+) progeny were recovered as Sb+ males *F from crosses of these males to cn1 bw1 (stock \#264) females and SM6a *F balanced stocks were established from crosses to cn1 bw1 Kr1/SM6a, *F bwk1 (stock \#3494) females. *F In this screen of ~9,500 progeny, we recovered 19 potential tandem *F duplications and 14 potential deletions in Sb+ males. The presence of a *F P-to-P deletion or tandem duplication was determined cytologically in *F polytene squash preparations of larvae from outcrossing males from a subset *F of the balanced stocks to Canton S females. One of eight potential tandem *F duplications tested was found to be duplicated cytologically. Five of ten *F potential deletions tested were found to be deleted (and four of these five *F deletions retained the miniwhite marker from P{lacW}). The remaining *F cytological preparations showed no cytologically visible aberrations. *F The single deletion line we retained has been named Df(2R)BSC3. It is *F maintained in a stock of genotype Df(2R)BSC3, w+mC unchk15501 cn1 *F bw1 sp1/SM6a, bwk1. By cytological criteria, it has the breakpoints *F 48E12-F4;49A11-B6. The precise molecular breakpoints in the regions of the *F two P insertions have not been determined; however, this deletion line *F retains the P{lacW} miniwhite marker, which could be used as a molecular *F marker for defining the sequence of the Df breakpoints. *F Note that the P{lacW}k15501 insertion is separable from the unchk15501 *F mutation based on the noncomplementation of unch and Df(2R)CX1. We *F confirmed the cytology of Df(2R)CX1 as 49C1-4;50C23-D2, which would leave *F at least six bands between the P insertion site and the proximal deletion *F breakpoint. Kania et al. (Genetics 139: 1663-1678, 1995) noted that the *F unch phenotype could not be reverted by excision of the P{lacW} insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132169 *a Sung *b C. *c S. *d Robinow *t 2000.11.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 27 20:56:03 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{EcR.GET-BD-GAL4}2 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Carl Sung and Steve Rabinow, University of Hawaii *F (11/00). P{EcR.GET-BD-GAL4}2 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132170 *a Andres *b A. *t 2000.11.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 27 21:18:37 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Sgs3-GFP} construct and insertions *F The following information accompanied stocks from Andy Andres, Northwestern *F U (11/00). The transgenic construct P{Sgs3-GFP} expresses a Sgs3-GFP *F fusion protein. P{Sgs3-GFP}2 and P{Sgs3-GFP}3 are homozygous viable and *F fertile insertions on the second and third chromosomes, respectively. The *F construct was described in the abstract FBrf0106146 and a full description *F is in press. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132171 *a Cook *b K. *t 2000.11.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Nov 28 18:10:46 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: In(3L)Na *F I examined the In(3R)Na deficient inversion chromosome from Bloomington stock *F 1443 In(3R)Na/Sb1 *F cytologically and saw a second inversion with breakpoints 66C9-E1;71B. It *F will be called In(3L)Na in our stocklist. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0132172 *a Bloomington Drosophila Stock Center *b ?. *t 2000.11.30 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Nov 30 14:38:43 2000 *F Subject: l(3)0054300543 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: l(3)0054300543 *F Dated: 30 November 2000 *F Information communicated: *F l(3)0054300543 is semi-lethal. # *U FBrf0132174 *a Moussian *b B. *t 2000.12.7 *T personal communication to FlyBase *u *F From bernard.moussian@tuebingen.mpg.de Thu Dec 07 14:29:04 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: l(3)j1B5 is a crb allele *F dear flybase, *F l(3)j1B5 (stock number 10331) is a new crumbs-allele, since it is not *F complemented by crb(2). *F Regards, *F \-- *F Dr. Bernard Moussian *F Max-Planck-Institut *F fuer Entwicklungsbiologie *F Abteilung III *F Spemannstr. 35 *F 72076 Tuebingen *F Germany *F Tel: \+49 7071 601492 *F Fax: \+49 7071 601 384 # *U FBrf0132176 *a Sackerson *b C. *t 2001.1.17 *T personal communication to FlyBase *u *F The following information was provided by Charles Sackerson on January 17 2001 *F in response to a curator query regarding the Adam and Apple genes: *F *F "CG12131 is "Adam" -- the sequence gives strong matches to eIF3. Although *F the end-points differ from those indicated by restriction maps of embryonic *F cDNAs, they are close, and intron-exon boundaries agree with the cDNAs. The *F 5' end maps to a constitutive DNaseI hypersensitive site. The region has *F been sequenced from a second species (Idiomyia grimshawi; Genbank U39659). *F *F The next gene, CG12134, was not detected in embryonic cDNAs (0-18 hr, *F Clontech). Both end-points map to constitutive hypersensitive sites, *F however. The predicted transcript overlaps an enhancer with pair-rule *F expression which has been proposed to be a redundant even-skipped late *F element. The predicted protein gives poor matches to other known proteins. *F *F The 5' end of eve is not predicted correctly; it has been mapped to *F nt131401. The 3' end (poly-A) and intron-exon boundaries are correct. *F *F Ter94 is the same as the transcript previously described as "Apple". The *F predicted 5' end is close to that indicated by sequences available in *F Genbank (AF047037 and AF202034), and maps to a DNaseI hypersensitive site *F found in embryos, adults, and S2 cells." # *U FBrf0132177 *a Gene Disruption Project members *b ?. *t 2001- *T personal communication to FlyBase *u *F BDGP Gene Disruption Project # *U FBrf0132178 *a Robertson *b K. *t 2001.1.31 *T personal communication to FlyBase *u *F Personal communication to FlyBase from Kathy Robertson *F *F Subject: P{UAS-GFP.nls}14 *F *F Date: Wed, 31 Jan 2001 09:29:48 -0500 *F From: Kathy Robertson *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-help@morgan.harvard.edu *F Subject: Mistake *F Content-Transfer-Encoding: 7bit *F *F Dear Sir/Madam, *F Please note the following mistake - which we discovered. *F *F According to your records - the stock: 4775 P{w[+mC]=UAS-GFP.nls}14 is in *F chromosomes 1; 3 *F *F It is actually on chromosomes 1;2. We have had written confirmation of *F this from Bruce Edgars Lab'. (see below). *F *F Thank you - Kathy Robertson. *F *F *F *F Subject: UAS-GFP nls 14 *F Date: Fri, 5 Jan 2001 12:26:43 -0800 (PST) *F From: Jung-Min Ko *F To: *F *F *F *F *F Hi, I'm a stock keeper at Edgar Lab. and have received your message which *F was forwarded to me from Bruce. I've checked out our stock list and found *F out that the line ( P (w+ UAS-GFP. nls)14 ) is on the second chromosome. I *F guess the stock center made a mistake. *F Thanks, *F Jungmin Ko # *U FBrf0132190 *a Whitfield *b E.J. *t 2000.12.15 *T personal communication to FlyBase *u *F From eleanor@ebi.ac.uk Fri Dec 15 10:34:14 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Fri, 15 Dec 2000 10:34:14 +0000 *F Date: Fri, 15 Dec 2000 10:34:49 +0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 [en] (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: Aubrey de Grey *F CC: Eleanor Whitfield , *F Michael Ashburner *F Subject: deleted protein_ids - part 1 *F Content-Transfer-Encoding: 7bit *F *F Hi, *F *F I have been working through the list of deleted protein_ids in the new *F release. In some cases when the gene/translation was deleted from *F release 1 it was not replaced by another gene/translation in release 2. *F For these we have deleted the TrEMBL entry as it is no longer supported *F by a translation. *F I have found that some of the protein_IDs still exist in the genes file *F so the following updates delete them. You may want to only delete the *F protein_ID and maintain the accession number - upto you, but please be *F sure to delete protein_ID and TrEMBL. *F For those cases where there is homology data are these lines being *F kept? I ask as it seems strange to keep them if you have no DNA or *F protein to generate the homology from, unless you want to keep it to *F maintain the history of the translation? *F *F updates follow: *F \*a CG15700 *F - *F \*g AE003808; AAF58058 *F \*m SPTREMBL:Q9V7J5 *F \# *F \*a CG16803 *F - *F \*g AE003800; AAF57743 *F \*m SPTREMBL:Q9V8C4 *F \# *F \*a CG17487 *F - *F \*g AE003752; AAF56486 *F \*m SPTREMBL:Q9VBP4 *F \# *F \*a CG15687 *F - *F \*g AE003731; AAF55787 *F \*m SPTREMBL:Q9VDK4 *F \# *F \*a CG11602 *F - *F \*g AE003677; AAF54186 *F \*m SPTREMBL:Q9VHW3 *F \# *F \*a CG11046 *F - *F \*g AE003677; AAF54182 *F \*m SPTREMBL:Q9VHW7 *F \# *F \*a CG17485 *F - *F \*g AE003667; AAF53900 *F \*m SPTREMBL:Q9VIL7 *F \# *F \*a CG4711 *F - *F \*g AE003652; AAF53550 *F \*m SPTREMBL:Q9VJJ5 *F \# *F \*a BG:DS02252.3 *F - *F \*g AE003648; AAF53486 *F \*m SPTREMBL:Q9VJN3 *F \# *F \*a CG16917 *F - *F \*g AE003631; AAF53090; AAF53091 *F \*m SPTREMBL:Q9VKH7 *F \*m SPTREMBL:Q9VKH8 *F not sure how Gillians pers comm for this gene should be treated as there *F is no adjacent gene to merge! *F \# *F \*a CG14278 *F - *F \*g AE003620; AAF52608 *F \*m SPTREMBL:Q9VLS6 *F \# *F \*a CG12353 *F - *F \*g AE003599; AAF51865 *F \*m SPTREMBL:Q9VNQ8 *F \# *F \*a CG18618 *F - *F \*g AE003598; AAF51839 *F \*m SPTREMBL:Q9VNT1 *F \# *F \*a CG15384 *F - *F \*g AE003584; AAF51287 *F \*m SPTREMBL:Q9VQ90 *F \# *F \*a CG3381 *F - *F \*g AE003577; AAF51039 *F \*m SPTREMBL:Q9VQX2 *F \# *F \*a CG10078 *F \*g AE003561; AAF50638; AAF50639 *F > *F \*g AE003561; AAF50639 *F - *F \*m SPTREMBL:Q9VRZ2 *F \# *F \*a CG18587 *F - *F \*g AE003546; AAF50097 *F \*m SPTREMBL:Q9VTF4 *F \# *F \*a CG13378 *F - *F \*g AE003519; AAF49232 *F \*m SPTREMBL:Q9VVS7 *F \# *F \*a CG18168 *F - *F \*g AE003519; AAF49217 *F \*m SPTREMBL:Q9VVU0 *F \# *F \*a CG11341 *F - *F \*g AE003481; AAF47888 *F \*m SPTREMBL:Q9VZD6 *F \# *F \*a CG9178 *F - *F \*g AE003471; AAF47488 *F \*m SPTREMBL:Q9W0G0 *F \# *F \*a CG10485 *F - *F \*g AE003462; AAF47153 *F \*m SPTREMBL:Q9W1B8 *F \# *F \*a CG18093 *F - *F \*g AE003460; AAF46996 *F \*m SPTREMBL:Q9W1Q7 *F \# *F \*a CG11478 *F - *F \*g AE003456; AAF46790 *F \*m SPTREMBL:Q9W2A2 *F \# *F \*a CG14433 *F - *F \*g AE003439; AAF46217 *F \*m SPTREMBL:Q9W3U6 *F \# *F \*a CG17679 *F - *F \*g AE003379; AAF45334 *F \*m SPTREMBL:Q9W5H3 *F \# *F \*a CG17452 *F - *F \*g AE003122; AAF45433 *F \*m SPTREMBL:Q9W5L5 *F \# *F *F thanks *F Ele *F *F From eleanor@ebi.ac.uk Fri Dec 15 10:40:38 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Fri, 15 Dec 2000 10:40:38 +0000 *F Date: Fri, 15 Dec 2000 10:41:13 +0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 [en] (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: Aubrey de Grey *F CC: Eleanor Whitfield , *F Michael Ashburner *F Subject: deleted protein_ids - part 2 *F Content-Transfer-Encoding: 7bit *F *F Hi again, *F *F These are the cases where a translation has been deleted in release 1 *F but has been replaced by a new/changed translation in release 2. *F In the genes file these are the simpler updates for this category of *F change - the protein_ID/TrEMBL accession may or may not be deleted so *F the updates complete the task. *F *F updates are: *F \*a CG18812 *F + *F \*m SPTREMBL:Q9V4R6 *F Q9V4R6 is now annotated to show CG18812 instead of CG12040 *F \# *F \*a CG8029 *F \*g AE003834; AAF58965; AAF58966 *F > *F \*g AE003834; AAF58966 *F - *F \*m SPTREMBL:Q9V555 *F splice variant associated with AAF58965 has been deleted by Celera. *F \# *F \*a CG18189 *F \*m SPTREMBL:Q9V627 *F > *F \*m SPTREMBL:Q9I7F3 *F \# *F \*a Vha100-2 *F - *F \*m SPTREMBL:Q9VE76 *F \*m SPTREMBL:Q9XZ27 *F secondary to Q9VE75 *F \*g AE003722; AAF55551; AAF55552 *F > *F \*g AE003722; AAF55552 *F AAF55551 is now dead and replaced by second version of AAF55552 *F \# *F \*a CG18749 *F + *F \*m SPTREMBL:Q9VHU6 *F \# *F \*a BG:DS09218.1 *F - *F \*g AE003650; AAF53529 *F \*m SPTREMBL:Q9VJL1 *F now secondary to Q9NK54 *F \# *F \*a CG18787 *F + *F \*m SPTREMBL:Q9I7N2 *F \# *F \*a CG10712 *F \*g AE003599; AAF51877; AAF51878 *F > *F \*g AE003599; AAF51877 *F AAF51878 protein_ID is deleted from the new release *F also why does this gene have 3 locations?! *F \*c 25C1 *F \*c Limits inferred from genome sequence *F \*c 79F5--6 *F \*c Limits inferred from genome sequence *F \*c 89E9--10 *F \*c Limits inferred from genome sequence *F \# *F \*a CG7611 *F \*g AE003594; AAF51739; AAG22180; AAG22181; AAG22182 *F > *F \*g AE003594; AAG22180; AAG22181; AAG22182 *F \# *F \*a slgA *F - *F \*m SPTREMBL:Q9VRH7 *F \*m SPTREMBL:Q9VRH9 *F secondary to Q9VRH8 *F \# *F \*a CG18768 *F \*m SPTREMBL:Q9VSA0 *F \*m SPTREMBL:Q9VSA1 *F > *F \*m SPTREMBL:Q9I7Q8 *F both are now secondary to Q9I7Q8 *F \# *F \*a Eip63F-1 *F \*m SPTREMBL:Q9VZL0 *F > *F \*m SPTREMBL:Q9I7T4 *F Q9VZL0 is secondary to Q9I7T4 *F \# *F \*a Sox14 *F - *F \*m SPTREMBL:Q9W1E3 *F secondary to Q9W1E2 *F \# *F \*a Caps *F - *F \*m SPTREMBL:Q9W5K5 *F secondary to Q9W5K6 *F \# *F \*a CG18769 *F \*m SPTREMBL:Q9VRX4 *F > *F \*m SPTREMBL:Q9I7Q7 *F Q9VRX4 is secondary to Q9I7Q7 *F \# *F *F thanks *F Ele *F *F From eleanor@ebi.ac.uk Fri Dec 15 10:50:18 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Fri, 15 Dec 2000 10:50:18 +0000 *F Date: Fri, 15 Dec 2000 10:50:52 +0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 [en] (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: Aubrey de Grey *F CC: Eleanor Whitfield , *F Michael Ashburner *F Subject: deleted protein_ids - part 3 *F Content-Transfer-Encoding: 7bit *F *F These are the more complicated updates from the previous category: *F translation has been deleted in release 1 but has been replaced by a *F new/changed translation in release 2. *F *F The genes file seems to have a few scenarios for the same category of *F change. *F 1) *F a CG has been 'eliminated' but it existed as a synonym of a known gene *F by previous work - these entries are fine, CG maintained, protein_ID and *F TrEMBL accession deleted in previous email *F 2) *F There are cases of a CG being eliminated and the new/changed translation *F has been assigned a new CG - for these cases I thought the original CG *F would become a synonym of the new CG? In some cases this appears to be *F true so for the following update I am simply reporting the problem but *F not suggesting the solution. *F They should either: *F look like this: *F \*a CG18474 *F \*z FBgn0037691 *F \*W Was AE003682; AAF54376; SPTREMBL:Q9VHD9 *F \*c 85D15--17 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)by416 (inferred from cytology) *F \*K Duplication: Dp(3;3)M86D[+]2 (inferred from cytology) *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F ie demoting \*g and \*m lines to \*W *F or become synonyms for those genes the translation matches - ie CG18474 *F could *F be a synonym of alpha-Man-II as AAF54375 and AAF54376 were splice *F variants of the same gene (although Celera incorrectly gave one of the *F variants a CG symbol)? *F *F the updates are: *F please note synonymy has been determined by sequence comparison or known *F splice variant. *F *F \*a CG17983 *F \*z FBgn0040781 *F \*c 43E4--5 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)tor-rx6 (inferred from cytology) *F \*K Duplication: Dp(2;Y)cn[+] (inferred from cytology) *F \*g AE003840; AAF59223 *F \*m SPTREMBL:Q9V4P3 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF59223 is now dead, replaced by second version of AAF59222 *F Q9V4P3 is now a secondary accession *F CG17983 become a synonym of BcDNA:GH02712? *F *F \*a CG2074 *F \*z FBgn0033200 *F \*c 43E5--7 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)tor-rx6 (inferred from cytology) *F \*K Duplication: Dp(2;Y)cn[+] (inferred from cytology) *F \*g AE003840; AAF59217 *F \*m SPTREMBL:Q9V4P8 *F \*j species == Arabidopsis thaliana; gene == 'putative ribotol *F dehydrogenase'; EMBL:AC004684; gi:3236237; score == 323; expect == *F 2.e-40 *F \*j species == Caenorhabditis elegans; gene == 'predicted using *F Genefinder; Similarity to dehydrogenases; cDNA EST'; EMBL:Z81035; *F protein_id:CAB02732; gi:3874345; score == 325; expect == 9.e-41 *F \*j species == Homo sapiens; gene == 'putative oxidoreductase'; *F gi:4758530; score == 128.4; expect == 1.e-09 *F \*j species == Mus musculus; gene == 'NAD(+)-dependent *F 15-hydroxyprostaglandin dehydrogenase'; EMBL:U44389; gi:1171436; score *F == 51.5; expect == 2.e-05 *F \*j species == Saccharomyces cerevisiae; gene == 'probable membrane *F protein YOR246c'; PIR:S67139; gi:2132926; score == 165.1; expect == *F 2.e-16 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*d lipid metabolism ; GO:0006629 ; score == 84 | inferred from *F electronic annotation *F \*F enzyme ; GO:0003824 ; score == 302 | inferred from electronic *F annotation *F \*f cell ; GO:0005623 ; score == 46.5 | inferred from electronic *F annotation *F \# *F AAF59217 is now dead, replaced by second version of AAF59216 *F Q9V4P8 is now a secondary accession *F CG2074 become a synonym of CG17986? *F *F \*a CG8698 *F \*z FBgn0033290 *F \*c 44C1 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)NCX10 (inferred from cytology) *F \*K Duplication: Dp(2;3)eve[1.18] (inferred from cytology) *F \*g AE003837; AAF59098 *F \*m SPTREMBL:Q9V4S7 *F \*j species == Caenorhabditis elegans; gene == 'gene unc-93 protein 2'; *F PIR:S23353; gi:102476; score == 52.7; expect == 2.e-06 *F \*j species == Homo sapiens; gene == 'dJ366N23.1 (putative C. elegans *F UNC-93 (protein 1, C46F11.1) LIK'; EMBL:AL021331; protein_id:CAA16149; *F gi:3355533; score == 57.8; expect == 5.e-08 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF59098 is now dead, replaced by second version of AAF59099 *F Q9V4S7 is now a secondary accession *F CG8698 become a synonym of CG2121? *F *F \*a CG13147 *F \*z FBgn0040757 *F \*c 49B7--8 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)vg-C (inferred from cytology) *F \*K Duplication: Dp(2;2)Y3b (inferred from cytology) *F \*g AE003821; AAF58473 *F \*m SPTREMBL:Q9V6F4 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF58473 is now dead, replaced by AAG22277 *F Q9V6F4 is now a secondary accession *F CG13147 become a synonym of CG8776? *F *F \*a CG13327 *F \*z FBgn0033796 *F \*c 49F6--7 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)vg-B (inferred from cytology) *F \*K Duplication: Dp(2;2)M14 (inferred from cytology) *F \*g AE003819; AAF58424 *F \*m SPTREMBL:Q9V6J9 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF58424 is now dead, replaced by second version of AAF58425 *F Q9V6J9 is now a secondary accession *F CG13327 become a synonym of CG17054? *F *F \*a CG13351 *F \*z FBgn0033892 *F \*c 50C22--23 *F \*c Limits inferred from genome sequence *F \*K Duplication: Dp(2;2)SMG45 (inferred from cytology) *F \*g AE003816; AAF58304 *F \*m SPTREMBL:Q9V6W4 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF58304 is now dead, replaced by AAG22270 *F Q9V6W4 is now a secondary accession *F CG13351 become a synonym of RN-tre? *F *F \*a CG12968 *F \*z FBgn0034043 *F \*c 52C7--8 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)WMG (inferred from cytology) *F \*K Duplication: Dp(2;2)SMG45 (inferred from cytology) *F \*g AE003809; AAF58106 *F \*m SPTREMBL:Q9V7F1 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF58106 is now dead, replaced by second version of AAF58107 *F Q9V7F1 is now a secondary accession *F CG12968 become a synonym of CG8242? *F *F \*a CG15900 *F \*z FBgn0033034 *F \*c 41F9 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2R)nap1 (inferred from cytology) *F \*K Duplication: Dp(2;2)BG (inferred from cytology) *F \*g AE003785; AAF57319 *F \*m SPTREMBL:Q9V9H0 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF57319 is now dead, replaced by second version of AAF57320 *F Q9V9H0 is now a secondary accession *F CG15900 become a synonym of CG11066? *F *F \*a CG5951 *F \*z FBgn0032989 *F \*c 40D2--3 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)lt2 (inferred from cytology) *F \*K Duplication: Dp(2;f)Bl (inferred from cytology) *F \*g AE003783; AAF57261 *F \*m SPTREMBL:Q9V9M5 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF57261 is now dead, replaced by second version of AAF57260 *F Q9V9M5 is now a secondary accession *F CG5951 become a synonym of CG3278? *F *F \*a CG10180 *F \*z FBgn0039082 *F \*c 95A2 *F \*c Limits inferred from genome sequence *F \*K Duplication: Dp(3;3)M95A[+]13 (inferred from cytology) *F \*g AE003743; AAF56140 *F \*m SPTREMBL:Q9VCL8 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF56140 is now dead, replaced by second version of AAF56139 *F Q9VCL8 is now a secondary accession *F CG10180 become a synonym of CG4370? *F *F \*a CG14287 *F \*z FBgn0038670 *F \*c 91E2--3 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)Cha9 (inferred from cytology) *F \*K Duplication: Dp(3;3)bxd[110] (inferred from cytology) *F \*g AE003724; AAF55619 *F \*m SPTREMBL:Q9VE14 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF55619 is now dead, replaced by second version of AAF55620 *F Q9VE14 is now a secondary accession *F CG14287 become a synonym of CG6003? *F *F \*a CG18479 *F \*z FBgn0037927 *F \*c 86E19--F1 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)M-Kx1 (inferred from cytology) *F \*K Duplication: Dp(3;3)M86D[+]2 (inferred from cytology) *F \*g AE003692; AAF54671 *F \*m SPTREMBL:Q9VGK6 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF54671 is now dead, the translation is overlapping with AAF54672 *F Q9VGK6 is now a secondary accession *F CG18479 become a synonym of CG14713? *F *F \*a CG9382 *F \*z FBgn0037706 *F \*c 85D23--25 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)by416 (inferred from cytology) *F \*K Duplication: Dp(3;3)M86D[+]2 (inferred from cytology) *F \*g AE003683; AAF54395 *F \*m SPTREMBL:Q9VHC1 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF54395 is now dead, replaced by second version of AAF54394 *F Q9VHC1 is now a secondary accession *F CG9382 become a synonym of CG9381? *F *F \*a CG11746 *F \*z FBgn0037598 *F \*c 85A3--4 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)CA3 (inferred from cytology) *F \*K Duplication: Dp(3;3)Tpl-J23 (inferred from cytology) *F \*g AE003679; AAF54253 *F \*m SPTREMBL:Q9VHP8 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF54253 is now dead, replaced by second version of AAF54252 *F Q9VHP8 is now a secondary accession *F CG11746 become a synonym of CG11745? *F *F \*a CG11743 *F \*z FBgn0037595 *F \*c 85A3--4 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)CA3 (inferred from cytology) *F \*K Duplication: Dp(3;3)Tpl-J23 (inferred from cytology) *F \*g AE003679; AAF54250 *F \*m SPTREMBL:Q9VHQ1 *F \*j species == Arabidopsis thaliana; gene == 'putative protein binding *F protein'; EMBL:AL035356; protein_id:CAA22992; gi:4220519; score == 294; *F expect == 6.e-79 *F \*j species == Caenorhabditis elegans; gene == F10B5.5; WP:CE01547; score *F == 240; expect == 2.e-62 *F \*j species == Homo sapiens; gene == 'HPV16 E1 protein binding protein'; *F EMBL:U96131; gi:2232019; score == 305; expect == 3.e-82 *F \*j species == Mus musculus; gene == Vcp; MGI:99919; score == 143.6; *F expect == 2.e-13 *F \*j species == Saccharomyces cerevisiae; gene == 'HYPOTHETICAL 60.5 KD *F PROTEIN IN MBA1-RPS13 INTERGENIC REGION'; SWP:P38126; gi:586317; score *F == 172; expect == 4.e-42 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126650 == Anonymous, 1999.11, curated genome annotation *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126650 == Anonymous, 1999.11, curated genome annotation *F \*F chaperone ; GO:0003754 | inferred from sequence similarity *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*d intracellular protein traffic ; GO:0006886 ; score == 262.6 | *F inferred from electronic annotation *F \*F endopeptidase ; GO:0004175 ; score == 212 | inferred from electronic *F annotation *F \*F enzyme ; GO:0003824 ; score == 262.2 | inferred from electronic *F annotation *F \*f cytosol ; GO:0005829 ; score == 587.7 | inferred from electronic *F annotation *F \# *F AAF54250 is now dead, replaced by second version of AAF54251 *F Q9VHQ1 is now a secondary accession *F CG11743 become a synonym of CG11744? *F *F \*a CG7553 *F \*z FBgn0037558 *F \*c 84F4--5 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3R)CA3 (inferred from cytology) *F \*K Duplication: Dp(3;3)D1 (inferred from cytology) *F \*g AE003678; AAF54203 *F \*m SPTREMBL:Q9VHU6 *F \*j species == Caenorhabditis elegans; gene == 'Y43F8B.4'; EMBL:AL032623; *F protein_id:CAA21512; gi:3947627; score == 165.4; expect == 8.e-19 *F \*j species == Homo sapiens; gene == 'prolyl 4-hydroxylase &agr; subunit *F (EC 1.14.11.2)'; EMBL:M24487; gi:190788; score == 132; expect == 3.e-30 *F \*j species == Mus musculus; gene == P4ha1; MGI:97463; score == 129; *F expect == 2.e-29 *F \*j species == Rattus; gene == 'PROLYL 4-HYDROXYLASE ALPHA SUBUNIT *F PRECURSOR'; SWP:P54001; gi:1709530; score == 130; expect == 8.e-30 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*F enzyme ; GO:0003824 ; score == 351 | inferred from electronic *F annotation *F \*f endoplasmic reticulum ; GO:0005783 ; score == 229 | inferred from *F electronic annotation *F \# *F AAF54203 is now dead, replaced with new translation AAG22134 *F Q9VHU6 now represents CG18749 *F CG7553 become a synonym of CG18749? *F *F \*a CG18092 *F \*z FBgn0032903 *F \*c 38F3--4 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)DS6 (inferred from cytology) *F \*K Duplication: Dp(2;f)Bl (inferred from cytology) *F \*g AE003668; AAF53944 *F \*m SPTREMBL:Q9VIH5 *F \*u Eliminated in the September 2000 Celera release of annotated sequence *F *F \# *F AAF53944 is now dead, replaced by second version of AAF53947 *F Q9VIH5 is now a secondary accession *F CG18092 become a synonym of CG18078? *F *F \*a CG18621 *F \*z FBgn0040997 *F \*c 38C6--8 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)pr76 (inferred from cytology) *F \*K Duplication: Dp(2;1)C239 (inferred from cytology) *F \*g AE003666; AAF53883 *F \*m SPTREMBL:Q9VIN4 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF53883 is now dead, replaced by second version of AAF53884 *F Q9VIN4 is now a secondary accession *F CG18621 become a synonym of spir? *F *F \*a CG10048 *F \*z FBgn0032792 *F \*c 37E4--5 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)E55 (inferred from cytology) *F \*K Duplication: Dp(2;1)C239 (inferred from cytology) *F \*g AE003663; AAF53810 *F \*m SPTREMBL:Q9VIV4 *F \*j species == Mus caroli; gene == 'RP2 protein, testosterone-regulated'; *F PIR:A39798; gi:109490; score == 127; expect == 1.e-28 *F \*j species == Mus musculus; gene == D7Rp2e; MGI:94203; score == 125; *F expect == 6.e-28 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF53810 is now dead, replaced by second version of AAF53809 *F Q9VIV4 is now a secondary accession *F CG10048 become a synonym of CG18094? *F *F \*a CG10441 *F \*z FBgn0032737 *F \*c 37B9 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)VA16 (inferred from cytology) *F \*K Duplication: Dp(2;1)C239 (inferred from cytology) *F \*g AE003661; AAF53737 *F \*m SPTREMBL:Q9VJ20 *F \*Y ATP-binding cassette transporter-like *F \*j species == Caenorhabditis elegans; gene == 'multidrug resistance *F related protein 1'; EMBL:U66260; gi:1518135; score == 1522.7; expect == *F 0 *F \*j species == Homo sapiens; gene == 'ABC transporter MOAT-B'; *F EMBL:AF071202; gi:3335173; score == 1967; expect == 0 *F \*j species == Mus musculus; gene == 'multidrug resistance protein'; *F EMBL:AF022908; gi:2511759; score == 1394; expect == 0 *F \*j species == Oryctolagus cuniculus; gene == 'multidrug *F resistance-associated protein 2'; EMBL:Z49144; protein_id:CAA89004; *F gi:1430907; score == 1612.4; expect == 0 *F \*j species == Saccharomyces cerevisiae; gene == 'cadmium resistance *F protein YCF1'; PIR:S51863; gi:1077042; score == 1341; expect == 0 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*x FBrf0126677 == Ketchum, 1999.11, curated genome annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*d small molecule transport ; GO:0006832 ; score == 998.6 | inferred *F from electronic annotation *F \*F ion channel ; GO:0005216 ; score == 3016.6 | inferred from electronic *F annotation *F \*f plasma membrane ; GO:0005886 ; score == 2818.9 | inferred from *F electronic annotation *F \*E FBrf0126677 == Ketchum, 1999.11, curated genome annotation *F \*F transporter ; GO:0005215 | inferred from sequence similarity *F \# *F AAF53737 is now dead, replaced by second version of AAF53736 *F Q9VJ20 is now a secondary accession *F CG10441 become a synonym of CG17338? *F *F \*a CG10666 *F \*z FBgn0032722 *F \*c 37B7--8 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)OD15 (inferred from cytology) *F \*K Duplication: Dp(2;1)C239 (inferred from cytology) *F \*g AE003661; AAF53721 *F \*m SPTREMBL:Q9VJ36 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF53721 is now dead, replaced by second version of AAF53722 *F Q9VJ36 is now a secondary accession *F CG10666 become a synonym of CG18397? *F *F \*a CG17937 *F \*z FBgn0032610 *F \*c 36A10 *F \*c Limits inferred from genome sequence *F \*K Deficiency: In(2L)b88e16 (inferred from cytology) *F \*K Duplication: Dp(2;2)SD5[rv3] (inferred from cytology) *F \*g AE003652; AAF53576 *F \*m SPTREMBL:Q9VJH3 *F \*Y diacylglycerol O-acyltransferase-like *F \*j species == Arabidopsis thaliana; gene == 'diacylglycerol *F O-acyltransferase'; EMBL:AJ131831; protein_id:CAB44774.1; gi:5050913; *F score == 224; expect == 5.e-58 *F \*j species == Caenorhabditis elegans; gene == 'cDNA EST yk453a2.3 comes *F from this gene; cDNA EST yk453a2.5 comes'; EMBL:Z75526; *F protein_id:CAA99773; gi:3874043; score == 277; expect == 5.e-74 *F \*j species == Homo sapiens; gene == 'ACAT related gene product 1'; *F EMBL:AF059202; gi:3746533; score == 282; expect == 2.e-75 *F \*j species == Mus musculus; gene == 'diacylglycerol acyltransferase'; *F EMBL:AF078752; gi:3859934; score == 279; expect == 2.e-74 *F \*j species == Saccharomyces cerevisiae; gene == 'acyl-CoA:sterol *F acyltransferase'; EMBL:U55383; gi:1389739; score == 113; expect == *F 1.e-24 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*x FBrf0126677 == Ketchum, 1999.11, curated genome annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*f plasma membrane ; GO:0005886 ; score == 41.8 | inferred from *F electronic annotation *F \*E FBrf0126677 == Ketchum, 1999.11, curated genome annotation *F \*F enzyme ; GO:0003824 | inferred from sequence similarity *F \# *F AAF53576 is now dead, replaced by second version of AAF53577 *F Q9VJH3 is now a secondary accession *F CG17937 become a synonym of CG17938? *F *F \*a CG14276 *F \*z FBgn0040956 *F \*c 28E4--7 *F \*c Limits inferred from genome sequence *F \*K Duplication: Dp(2;3)dp[h27] (inferred from cytology) *F \*g AE003620; AAF52602 *F \*m SPTREMBL:Q9VLT2 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF52602 is now dead, replaced by second version of AAF52603 *F Q9VLT2 is now a secondary accession *F CG14276 become a synonym of CG8683? *F *F \*a CG13774 *F \*z FBgn0031870 *F \*c 27C4--6 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)Dwee-&dgr;5 (inferred from cytology) *F \*K Duplication: Dp(2;2)C619 (inferred from cytology) *F \*g AE003615; AAF52459 *F \*m SPTREMBL:Q9VM66 *F \*j species == Caenorhabditis elegans; gene == 'F35D11.11 gene product'; *F EMBL:U29381; gi:868224; score == 255.9; expect == 2.e-21 *F \*j species == Entamoeba histolytica; gene == 'myosin heavy chain'; *F EMBL:L03534; gi:1850913; score == 283.6; expect == 2.e-27 *F \*j species == Homo sapiens; gene == 'myosin &bgr; heavy chain, cardiac *F and skeletal muscle'; PIR:S12458; gi:107132; score == 207.1; expect == *F 1.e-22 *F \*j species == Mus musculus; gene == Myhca; MGI:97255; score == 200.7; *F expect == 2.e-22 *F \*j species == Saccharomyces cerevisiae; gene == 'integrin homolog'; *F PIR:S30782; gi:320776; score == 112; expect == 3.e-23 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*d cytoskeleton organization and biogenesis ; GO:0007010 ; score == *F 2120.7 | inferred from electronic annotation *F \*F motor ; GO:0003774 ; score == 3255.5 | inferred from electronic *F annotation *F \*f cytoskeleton ; GO:0005856 ; score == 5960.9 | inferred from *F electronic annotation *F \# *F AAF52459 is now dead, replaced by second version of AAF52458 *F Q9VM66 is now a secondary accession *F CG13774 become a synonym of CG18304? *F *F \*a CG14576 *F \*z FBgn0037111 *F \*c 78E5--F1 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3L)XS1155 (inferred from cytology) *F \*g AE003595; AAF51759 *F \*m SPTREMBL:Q9VP03 *F \*j species == ACICA; gene == 'ALDOSE 1-EPIMERASE PRECURSOR *F (MUTAROTASE)'; SWP:P05149; gi:127542; score == 69.9; expect == 7.e-12 *F \*j species == Caenorhabditis elegans; gene == 'similar to aldose *F 1-epimerases (Pfam:PF01263, Score=216.9, E=3.1e'; EMBL:AF125952; *F protein_id:AAD14698; gi:4262570; score == 54.7; expect == 3.e-07 *F \*j species == Homo sapiens; gene == 'Ibd1'; EMBL:U11036; gi:836883; *F score == 47.3; expect == 4.e-05 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*d carbohydrate metabolism ; GO:0005975 ; score == 44.9 | inferred from *F electronic annotation *F \*F enzyme ; GO:0003824 ; score == 44.9 | inferred from electronic *F annotation *F \*f cytoplasm ; GO:0005737 ; score == 44.9 | inferred from electronic *F annotation *F \# *F AAF51759 is now dead, replaced by second version of AAF51760 *F Q9VP03 is now a secondary accession *F CG14576 become a synonym of CG12562? *F *F \*a CG17647 *F \*z FBgn0031363 *F \*c 22B2 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(2L)frtz11 (inferred from cytology) *F \*K Duplication: Dp(2;Y)odd[4.13] (inferred from cytology) *F \*g AE003585; AAF51340 *F \*m SPTREMBL:Q9VQ42 *F \*j species == Homo sapiens; OMIM:603076; score == 83.1; expect == 1.e-15 *F *F \*j species == Mus musculus; gene == Abc8; MGI:107704; score == 84.7; *F expect == 4.e-16 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*d small molecule transport ; GO:0006832 ; score == 84.7 | inferred from *F electronic annotation *F \*F enzyme ; GO:0003824 ; score == 84.7 | inferred from electronic *F annotation *F \*F ion channel ; GO:0005216 ; score == 84.7 | inferred from electronic *F annotation *F \*F ligand binding or carrier ; GO:0005488 ; score == 84.7 | inferred *F from electronic annotation *F \*f plasma membrane ; GO:0005886 ; score == 84.7 | inferred from *F electronic annotation *F \# *F AAF51340 is now dead, replaced by second version of AAF51341 *F Q9VQ42 is now a secondary accession *F CG17647 become a synonym of CG17646? *F *F \*a CG17981 *F \*z FBgn0036167 *F \*c 68C4--5 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3L)vin66 (inferred from cytology) *F \*K Duplication: Dp(3;3)M67C[+]4 (inferred from cytology) *F \*g AE003544; AAF50057 *F \*m SPTREMBL:Q9VTJ1 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F remove protein_ID and TrEMBL accession *F *F \*a CG14139 *F \*z FBgn0040819 *F \*c 68C7--8 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3L)vin66 (inferred from cytology) *F \*K Duplication: Dp(3;3)M67C[+]4 (inferred from cytology) *F \*g AE003544; AAF50048 *F \*m SPTREMBL:Q9VTK0 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF50048 is now dead, replaced by second version of AAF50047 *F Q9VTK0 is now a secondary accession *F CG14139 become a synonym of CG6100? *F *F \*a CG18230 *F \*z FBgn0036797 *F \*c 75D1 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3L)Cat (inferred from cytology) *F \*K Duplication: Dp(3;3)M35 (inferred from cytology) *F \*g AE003520; AAF49252 *F \*m SPTREMBL:Q9VVQ8 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF49252 is now dead, replaced by second version of AAF49251 *F Q9VVQ8 is now a secondary accession *F CG18230 become a synonym of CG13382? *F *F \*a CG6918 *F \*z FBgn0036957 *F \*c 77A4--B1 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3L)rdgC-co2 (inferred from cytology) *F \*K Duplication: Dp(3;3)M72 (inferred from cytology) *F \*g AE003514; AAF49049 *F \*m SPTREMBL:Q9VW99 *F \*j species == Caenorhabditis elegans; gene == 'cDNA EST yk257c2.5 comes *F from this gene; cDNA EST yk257c2.3 comes'; EMBL:Z79598; *F protein_id:CAB01869; gi:3979721; score == 54.3; expect == 2.e-06 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF49049 is now dead, replaced by second version of AAF49048 *F Q9VW99 is now a secondary accession *F CG6918 become a synonym of CG17233? *F *F \*a CG6266 *F \*z FBgn0036971 *F \*c 77B3--4 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(3L)rdgC-co2 (inferred from cytology) *F \*K Duplication: Dp(3;3)M72 (inferred from cytology) *F \*g AE003514; AAF49031 *F \*m SPTREMBL:Q9VWB5 *F \*Y serpin *F \*j species == Homo sapiens; OMIM:600517; score == 73; expect == 1.e-12 *F \*j species == Mus musculus; gene == At3; MGI:88095; score == 68.3; *F expect == 4.e-11 *F \*j species == Ovis aries; gene == 'ANTITHROMBIN-III PRECURSOR (ATIII)'; *F SWP:P32262; gi:416622; score == 62.8; expect == 2.e-09 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126651 == Ashburner, 1999.11, curated genome annotation *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126651 == Ashburner, 1999.11, curated genome annotation *F \*F serpin ; GO:0004868 | inferred from sequence similarity *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*F enzyme inhibitor ; GO:0004857 ; score == 342.9 | inferred from *F electronic annotation *F \# *F AAF49031 is now dead, replaced by second version of AAF49032 *F Q9VWB5 is now a secondary accession *F CG6266 become a synonym of CG6663? *F *F \*a CG1579 *F \*z FBgn0030335 *F \*c 10E2--3 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(1)HA85 (inferred from cytology) *F \*K Duplication: Dp(1;2)v[+]65b (inferred from cytology) *F \*g AE003487; AAF48096 *F \*m SPTREMBL:Q9VYU2 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \*x FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*E FBrf0126705 == FamiliarityBreedsContempt, 1999.11, automatic genome *F annotation *F \*F chaperone ; GO:0003754 ; score == 122.6 | inferred from electronic *F annotation *F \*F enzyme ; GO:0003824 ; score == 61.3 | inferred from electronic *F annotation *F \*f endoplasmic reticulum ; GO:0005783 ; score == 61.3 | inferred from *F electronic annotation *F \# *F AAF48096 is now dead, it was contained within AAF48095 *F Q9VYU2 is now a secondary accession *F CG1579 become a synonym of Hsc70-3? *F *F thanks *F Ele *F From eleanor@ebi.ac.uk Mon Dec 18 12:00:10 2000 *F Envelope-to: ag24@gen.cam.ac.uk *F Delivery-date: Mon, 18 Dec 2000 12:00:10 +0000 *F Date: Mon, 18 Dec 2000 12:00:45 +0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 [en] (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: Aubrey de Grey *F CC: Eleanor Whitfield *F Subject: Re: remaining eliminated genes *F Content-Transfer-Encoding: 7bit *F *F this update (and the earlier messages) should take care of all *F protein_IDs and TrEMBL accession in 'eliminated' genes. *F when you have done these could you do one last check? (sorry to be *F paranoid!) *F *F \*a BG:DS02252.4 *F + *F \*g AE003648 *F to treat the same as the others, ie delete protein_ID, keep accession *F number *F \# *F \*a BG:DS09218.1 *F \*g AE003650; AAF53529 *F > *F \*g AE003650 *F protein_ID is dead *F \# *F \*a Cyp318a1 *F \*g AE003488; AAF48137 *F > *F \*g AE003488 *F protein_ID is dead *F \# *F \*a CG13945 *F \*g AE003815; AAF58278 *F \*m SPTREMBL:Q9V6Y9 *F > *F \*g AE003815 *F protein_ID is dead *F \# *F AAF46284 is dead and the translation is exactly matched by AAF46285 *F so: *F \*a CG18624 *F \+ *F \*i CG15328 *F \*m SPTREMBL:Q9W3N7 *F \# *F \*a CG8810 *F \*g AE003821; AAF58480 *F \*m SPTREMBL:Q9V6E7 *F > *F \*g AE003821 *F protein_ID is dead *F \# *F *F \*a CG16994 *F \*z FBgn0029544 *F \*c 1C5--D1 *F \*c Limits inferred from genome sequence *F \*K Deficiency: Df(1)tR15 (inferred from cytology) *F \*K Duplication: Dp(1;2)E1 (inferred from cytology) *F \*g AE003419; AAF45567 *F \*m SPTREMBL:Q9W5C3 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F AAF45567 is now dead and replaced by AAG22365 *F please merge CG16994 and CG18830, deleting AAF45567 *F \*a CG18830 *F \*z FBgn0042153 *F \*W New in Celera 0007 dump. ag000818 *F \*c 1C5--D1 *F \*c Limits inferred from genome sequence *F \*g AE003419; AAG22365 *F \# *F *F \*a CG17937 *F \*g AE003652; AAF53576 *F \*m SPTREMBL:Q9VJH3 *F \*u Eliminated in the July 2000 Celera release of annotated sequence. *F \# *F from previous email: *F AAF53576 is now dead, replaced by second version of AAF53577 *F Q9VJH3 is now a secondary accession *F CG17937 become a synonym of CG17938? *F *F thanks *F Ele # *U FBrf0133187 *a Pradel *b J. *t 2001.1.25 *T personal communication to FlyBase *u *F Date: Thu, 25 Jan 2001 09:05:57 \+0100 *F To: Chihiro Yamada *F From: pradel *F Subject: Re: FlyBase Query (cy1009) *F Dear Chihiro, *F Thanks for your message. I like your suggestion to take rept for reptin and *F pont for pontin. *F Personal communication of Jacques Pradel to Flybase: *F I am writing about the symbols to be used in Flybase for the Drosophila *F genes reptin and pontin (Bauer et al. 2000, EMBO J. 19, 6121-6130). We did *F not taken rep and pon since these symbols are already used for Rab escort *F protein (Dong et al., 1999, Biochim. Biophys. Acta 1449, 194-198) and *F partner of numb (Lu et al. 2000, Cell 95, 225-235), respectively. Instead *F of 'dpon' and 'drep', which does not obey the Flybase policy to drop any *F prefixes denoting Drosophila and has unevenly been used in the EMBO paper, *F I suggest Flybase to adopt the symbols 'rept' and 'pont' for 'reptin' and *F 'pontin', respectively. *F Thanks again and best wishes, *F Jacques *F At 18:21 24/01/01 \+0000, you wrote: *F >Dear Dr Pradel, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Bauer et al., 2000, EMBO J. 19(22): 6121--6130 *F > *F >I have a question about the symbols you gave to your new Drosophila *F >genes, pontin and reptin. *F > *F >In your paper you either used the symbols drep and dpon or rep and *F >pon. I assume the 'd' denotes 'drosophila'. In FlyBase we have the *F >policy of dropping any prefixes denoting Drosphila when assigning *F >FlyBase symbols. I append an old posting from Thom Kaufman explaining *F >this policy. *F > *F >This leave the symbols rep and pon. Unfortunately the symbols Rep and *F >pon are already being used as FlyBase symbols. I hope you agree that *F >this is potentially very confusing. *F > *F >Rep *F >Rab escort protein *F >CG8432 *F >FBrf0114616 == Dong, 1998.11.7, GenBank/EMBL/DDBJ: AF105063 *F >FBrf0108170 == Dong et al., 1999, Biochim. biophys. Acta 1449(2): 194--198 *F > *F >pon *F >partner of numb *F >CG3346 *F >FBrf0105275 == gm5411.h == Lu et al., 1998, Cell 95(2): 225--235 *F >FBrf0117559 == Lu, 1998.9.10, GenBank/EMBL/DDBJ: AF091050 *F > *F >Although the (differently capitalised) symbols rep and Pon are not *F >taken, given the modern convention of capitalising the first letter *F >when referring to the gene products, we try to avoid having symbols *F >using the same letters but differently capitalised. *F > *F >So I writing to ask you whether you would consider changing the symbol *F >you use to describe the pontin and reptin genes. At the moment the two *F >genes have the FlyBase symbols 'pontin' and 'reptin', i.e. their *F >FlyBase gene names and their FlyBase gene symbols are the same. The *F >choice of new symbol is, of course, yours, but two symbols that I have *F >checked we do not have already are 'Pont' and 'Rept'. Whatever the *F >FlyBase symbol becomes the symbols 'pon' and 'rep' will be recorded in *F >FlyBase as being aliases, so anyone reading your EMBO Journal paper *F >will be able to find the genes based on their symbol. *F > *F >Could you email me to confirm your choice of symbol, I would archive *F >this email as a personal communication from you to FlyBase so that we *F >know why the symbols were chosen. *F > *F >Best wishes, *F > *F >Chihiro *F > *F Jacques Pradel *F Laboratoire de Genetique et Physiologie du Developpement *F IBDM, case 907 *F Campus de Luminy *F 13288 Marseille Cedex 9 *F FRANCE *F Tel: 33 (0)4 91 26 96 07 *F Secretariat (Mme Patricia Alleman): 33 (0)4 91 26 96 00 *F Fax: 33 (0)4 91 82 06 82 *F E-mail: pradel@lgpd.univ-mrs.fr *F http://ibdm.univ-mrs.fr # *U FBrf0133205 *a Wismar *b J. *t 2001.1.4 *T personal communication to FlyBase *u *F Dear Dr Wismar, *F I am currently curating your paper for FlyBase: *F Wismar et al., 2000, Dev. Biol. 226(1): 1--17 *F Does the EMS allele wocrdg the same allele as the single EMS allele *F mentioned in your abstract from 13th EDRC meeting in Crete? *F Wismar et al., 1993, Europ. Dros. Res. Conf. 13: Poster I6 *F Best wishes, *F Chihiro *F \--------- *F Date: Wed, 3 Jan 2001 14:24:05 \+0100 *F To: cy200@gen.cam.ac.uk *F From: Jasmine Wismar *F Subject: woc allele *F Dear Chihiro, *F The allel woc rgl is not the same as the one in the abstract of 1993 which *F was found in a P-element mutagenesis (not EMS allele as you wrote) but does *F not contain a P-lac W insertion. This allel is now called woc st1 as *F mentioned in materials and methods. *F Sincerely *F Jasmine *F \--------- *F To: cy200@gen.cam.ac.uk, wismar@mail.uni-mainz.de *F Subject: Re: woc allele *F From: Chihiro Yamada *F Date: Wed, 3 Jan 2001 13:59:10 \+0000 *F Dear Jasmine, *F Thanks for your reply. So if I understand you correctly, the wocst1 *F allele is one of the nine woc alleles found 'during an 'enhancer trap' *F mutagenesis' as mentioned in the 1993 abstract. Is this right? *F In the next sentence 'One is EMS induced', suggests that you also had *F at that time another single EMS induced woc allele. Does this still *F exist? Have you given it a name? The reason I ask is that in looking *F through our current records, up until your recent paper, we only had *F one woc allele recorded \- woc1. It seems that this was created by us *F in 1993, based on the statement that describes a single unnamed allele *F i.e. 'One is EMS induced'. The name was given arbitrarily by us at *F the time (we no longer name unnamed alleles in this way), I would like *F to change it to a name assigned by you. Do you have one I can used? *F Thanks *F Chihiro *F \------- *F Date: Thu, 4 Jan 2001 10:51:18 \+0100 *F To: Chihiro Yamada *F From: Jasmine Wismar *F Subject: Re: woc allele *F Dear Chihiro, *F I know it is confusing with the woc alleles. *F We have one EMS induced allel called woc rgl (Dev. Bio. 226, 2000) this was *F the one mentioned as 'one is EMS induced' in 1993. All the phenotype *F described in this abstract deals about the sterile alleles thus I thought *F you have been talking about these alleles in your letter. Sorry for this . *F The nine alleles from the enhancer trap mutagenesis seem to be just one *F allele as all the PlacW insertions have been at different places on the *F third chromosome. Separating the mutation (not yet identified on sequence *F level) from the PlacW insertions by crossing over all the 'alleles' show *F the same phenotype and have white eyes. Thus we suppose that the nine *F alleles are just one allele which we called woc st1, but this has to be *F confirmed by sequence analysis (on the way). *F I hope that now the situation is clear otherwise ask again. *F Sincerely *F Jasmine # *U FBrf0133211 *a Neufeld *b T. *t 2001.1.11 *T personal communication to FlyBase *u *F Date: Thu, 11 Jan 2001 09:34:24 \-0600 *F From: Thomas Neufeld *F Reply-To: neufeld@mail.ahc.umn.edu *F Organization: Department of Genetics, Cell Biology, and Development *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (cy972) *F Dear Chihiro, *F PTEN-MGH1 was generated in Iswar Hariharan's lab, and is unpublished. It *F is an ems allele, and fails to complement PTEN-DJ1. Please cite *F Hariharan as the source. *F Thanks, *F Tom *F _________________________ *F Thomas P. Neufeld, PhD *F Assistant Professor *F University of Minnesota *F Department of Genetics, Cell Biology, and Development *F Room 6-160 Jackson Hall *F 321 Church Street S.E. *F Minneapolis, MN 55455 *F TEL: 612-625-5158 *F FAX: 612-626-7031 *F Chihiro Yamada wrote: *F > *F > Dear Dr Neufeld, *F > *F > I am currently curating your paper for FlyBase: *F > *F > Zhang et al., 2000, Genes Dev. 14(21): 2712--2724 *F > *F > I have a quick question I was hoping you could answer for me. *F > *F > On p2716 you mention an allele of dPTEN you call MGH1. We do not have *F > an allele of dPTEN in our records with this name. Is it a new allele? *F > If not could you give me s a few details about this allele? e.g. Has it *F > been published before, if so where. If it is a new allele can you give *F > me some other details, e.g. how was it made etc. *F > *F > Any new information you give me will be curated as a personal *F > communication from you and FlyBase, if thats O.K. with you. *F > *F > Best wishes, *F > *F > Chihiro. *F > *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: c.yamada@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \---------------------------------------------------------------------- *F \-- # *U FBrf0133216 *a So *b C. *c L. *d Rabinow *t 2000.12.9 *T personal communication to FlyBase *u *F From Leonard.Rabinow@emex.u-psud.fr Wed Dec 20 09:21:50 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 20 Dec 2000 09:21:50 \+0000 *F X-Sender: rabino41@ultra.u-psud.fr *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Transfer-Encoding: quoted-printable *F Date: Wed, 20 Dec 2000 10:20:39 \+0100 *F To: Gillian Millburn (Genetics) *F From: Leonard Rabinow *F Subject: Re: FlyBase query *F Content-Length: 4305 *F Hello Gillian, *F >I am curating your paper for FlyBase: *F >FBrf0130184 == Yun et al., 2000, Genetics 156(2): 749--761 *F >3. FlyBase has a record of a gene called 'Msu' ('Mosaic suppressor') *F >from the paper: *F > *F >Csink et al., 1994, Genetics 136(2): 573--583 *F > *F >In that paper they describe 2 alleles 'Msu' and 'Msu2' which they say *F >were originally isolated in mutagenesis screens described in: *F > *F >Birchler et al., 1989, Genes Dev. 3: 73--84 *F >Rabinow et al., 1991, Genetics 129: 463--480 *F > *F >In the Csink Genetics paper 'Msu' and 'Msu2' are said to be on 3rd *F >chromosomes with a number of breaks, including one at 98F and one near *F >centric heterochromatin in both cases. The 98F breakpoint is suggested *F >to be responsible for the mutant phenotype. They test for *F >complementation and say that Doa and Msu are discrete complementation *F >groups. *F > *F >I am writing to ask whether you know if the 'Msu' and 'Msu2' *F >mutations from the Csink Genetics paper are the same mutations as the *F >Doa alleles you call 'Msu' and 'Msu2' respectively in Table 2 of your *F >2000 Genetics paper. *F Yes, these are all one and the same. We have irrefutable evidence that Msu *F and Msu(2) are position-effected alleles of Doa. ... Csink and Birchler *F did not test as wide a range of alleles as we did, and there are several *F Doa-Msu (and Rem, yet another class- hence the Rem(gammaA)) alleles which *F do NOT complement. We examined all the Rem and Msu alleles for breaks in *F Doa using Southerns- there are none. However, some but not all of the Doa *F RNAs are strongly reduced in both Msu and Rem alleles, and the effects of *F both are suppressed by suppressors of PEV. If you care to cite this as a *F personal communication to Flybase, please feel free, but it should be cited *F as from Chi So and Leonard Rabinow (or however you abbreviate first names). *F I interpret the differences in allelic specificity reported by Csink and *F Birchler of Msu alleles on other TE-induced white alleles as almost *F certainly due to PEV effects on other loci nearby to Doa also affected in *F the chromsomal rearrangements. *F >I think that given the location of the genes, the overlap of authors on *F >the papers and the fact that in the Materials and Methods of your 2000 *F >Genetics paper you say that 'DoaMsu2 complements the vast majority of *F >other Doa mutations' that Msu = DoaMsu and Msu2 = DoaMsu2, but I *F >wanted to check before merging the two genes in FlyBase, *F See above. It would seem reasonable. *F >I look forward to hearing from you, *F I hope this was helpful. Please don't hesitate if you need any further *F information or clarification, or I can provide any help in any way. *F Best wishes, *F Lenny Rabinow *F \-------------------------------------------------------------------- *F Leonard Rabinow *F Laboratoire d'Embryologie Moléculaire *F Bâtiment 445 *F Université Paris XI *F 91405 ORSAY CEDEX *F FRANCE *F Leonard.Rabinow@emex.u-psud.fr *F Phone: 1-69-15-74-65 *F FAX: 1-69-15-68-02 *F (from US, prefix: 011-33) # *U FBrf0133220 *a Drysdale *b R. *t 2000.12.12 *T personal communication to FlyBase *u FlyBase error report for CG8110 on Tue Dec 12 11:10:57 2000. *F From rd120@gen.cam.ac.uk Tue Dec 12 11:12:43 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 12 Dec 2000 11:12:43 \+0000 *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report for CG8110 on Tue Dec 12 11:10:57 2000 *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Tue, 12 Dec 2000 11:12:28 \+0000 *F Content-Length: 394 *F Error report from Rachel Drysdale (r.drysdale@gen.cam.ac.uk) *F Gene or accession: CG8110 *F Release: 1 *F Gene annotation error *F Genes CG8110 and syd should be merged. *F Comments: Location of CG8110 (FBgn0035826), orientation with respect to *F pbl (FBgn0003041), identity with GH19969 and sequence alignment of *F encoded protein with that in AF262045 indicate that CG8110 corresponds *F to syd (FBgn0024187). # *U FBrf0133221 *a Mount *b S. *c H. *d Mookherjee *t 2000.12.14 *T personal communication to FlyBase *u FlyBase error report for CG1693 on Thu Dec 14 13:11:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Dec 14 23:32:58 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 14 Dec 2000 23:32:58 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 14 Dec 2000 13:11:34 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sm193@umail.umd.edu *F Subject: FlyBase error report for CG1693 on Thu Dec 14 13:11:19 2000 *F Content-Length: 919 *F Error report from Steve Mount and Himan Mookherjee (sm193@umail.umd.edu) *F Gene or accession: CG1693 *F Gene annotation error *F Gene CG1693 has incorrect exon/intron structure. *F Comments: There is a microexon (12 nt.) not annotated in the genomic sequence *F AE003568.2 GI:10726967, but clearly present based on the sequence of the cDNA *F AF184227. The exon occurs at 105,896-105,907, and has the sequence *F GTCCCAATAATG (CATTATTGGGAC on the complementary strand, which is represented *F by AE003568.2). In addition, the 3' splice site annotation must be changed, so *F that *F ..105824, 106727.. becomes ..105814, 105896-105907, 106729.. *F Prediction of protein sequence AAD55738.1 requires that this microexon be *F accounted for. The sequence of AAF50831.1 (predicted from the genomic *F sequence) differs from AAD55738 (which is based on a cDNA). *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133222 *a Benos *b T. *t 2000.12.13 *T personal communication to FlyBase *u FlyBase error report on Wed Dec 13 14:35:56 2000. *F From benos@ebi.ac.uk Wed Dec 13 14:36:42 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 13 Dec 2000 14:36:42 \+0000 *F Date: Wed, 13 Dec 2000 14:35:56 \+0000 (GMT) *F From: Takis Benos *F To: flybase-updates@morgan.harvard.edu *F cc: Eleanor Whitfield *F Subject: EDGP-CG gene comparison.... *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='1053048513-1391734921-976718156=:45284' *F Content-Length: 63419 *F Hello, dear All: *F Please, find attached a text file with the results of the comparison we *F performed between EDGP genes and the ones reported by Celera in the version *F 1.0 of the Drosophila Genome. *F The differences between the genes are divided in the following categories: *F \# 0. less than 1% differences \--> IDENTICAL *F \# A. differences on ATG selection *F \# B. additional/missing internal exon *F \# C. differences on termination codon selection *F \# D. major differences indicating very different gene model *F Genes of category D were analysed in detail (as well as some of the other *F categories). File EDGPvsCG.takis.for_FB contains the results of the *F analysis. Note that some of the CG genes (category D) were found to be *F wrong, based on EST hits. Also two of the genes were 'splitted' between *F two EDGP clones; therefore their corresponding entries need to be joined. *F Best regards, *F takis ;) *F From benos@stemloop.wustl.edu Wed Dec 13 14:32:39 2000 *F Date: Wed, 13 Dec 2000 08:30:26 \-0600 *F From: Takis Benos *F To: benos@ebi.ac.uk *F \# *F \#23456789012345678901234567890123456789012345678901234567890123456789012345 *F \# *F \# This file is summarising the differences between EDGP and CG *F \# a.a. sequences, as reported by Melanie Gatt in the form of ClustalW *F \# output. THe categories of differences that are considered are: *F \# *F \# 0. less than 1% differences \--> IDENTICAL *F \# *F \# A. differences on ATG selection *F \# B. additional/missing internal exon *F \# C. differences on termination codon selection *F \# D. major differences indicating very different gene model *F \# *F \# Plus/minus symbols mean that EDGP gains/misses in sequence length over CG *F \# gene. *F \# *F \# *F \# PVB-000724 *F \# *F \# *F \# *F >CG17636|FBan0017636|CT38919|FBan0017636 last_updated:000321 *F >EG:23E12.1 *F >Diff : 0 *F >CG17617|FBan0017617|CT38862|FBan0017617 last_updated:000321 *F >EG:23E12.5 *F >Diff : 0 *F >CG17960|FBan0017960|CT40020|FBan0017960 last_updated:000321 *F >EG:23E12.2 *F >Diff : B- *F >CG17707|FBan0017707|CT39230|FBan0017707 last_updated:000321 *F >EG:23E12.3 *F >Diff : 0 *F >CG3038|FBan0003038|CT10170|FBan0003038 last_updated:000321 *F >EG:BACR37P7.1 *F >Diff : 0 *F >CG2995|FBan0002995|CT10081|FBan0002995 last_updated:000321 *F >EG:BACR37P7.2 *F >Diff : 0 *F >CG13377|FBan0013377|CT32709|FBan0013377 last_updated:000321 *F >EG:BACR37P7.9 *F >Diff : A- *F >CG13375|FBan0013375|CT32707|FBan0013375 last_updated:000321 *F >EG:BACR37P7.8 *F >Diff : B- *F >CG18104|FBan0018104|CT40671|FBan0018104 last_updated:000321 *F >EG:171D11.4 *F >Diff : C+ *F >CG17829|FBan0017829|CT39573|FBan0017829 last_updated:000321 *F >EG:115C2.6 *F >Diff : 0 *F >CG5254|FBan0005254|CT16777|FBan0005254 last_updated:000321 *F >EG:BACR19J1.2 *F >Diff : 0 *F >CG5273|FBan0005273|CT16821|FBan0005273 last_updated:000321 *F >EG:BACR19J1.3 *F >Diff : 0 *F >CG12467|FBan0012467|CT32681|FBan0012467 last_updated:000321 *F >EG:34F3.2 *F \#>Diff : A-- B- C+ *F >Diff : D *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are probably due to different prediction *F \# methods used: in this case gene CG12467 should be EG:34F3.1 \+ *F \# EG:34F3.2. *F \# B : CG12467 has one additional exon: this probably comes from Genie *F \# prediction. None of Genfinder/Genscan predictions support this exon. *F \# C : differences on the \-COOH are probably due to different prediction *F \# methods used. In this case, the two last exons of EG:34F3.2 (starting *F \# 'APSATPII...' should form another CG gene). *F \# *F \# There is no supporting evidence to favour of EDGP or CG model. *F \# (and the EST hits cannot enlighten us further on that) *F \# *F \#CONCLUSION: Ambigious *F \# *F >CG16785|FBan0016785|CT32689|FBan0016785 last_updated:000321 *F >EG:34F3.6 *F >Diff : A-- *F >CG11664|FBan0011664|CT34394|FBan0011664 last_updated:000321 *F >EG:BACR7A4.3 *F >Diff : 0 *F >CG3711|FBan0003711|CT12449|FBan0003711 last_updated:000321 *F >EG:BACR7A4.19 *F >Diff : 0 *F >CG3034|FBan0003034|CT10206|FBan0003034 last_updated:000321 *F >EG:BACR7A4.6 *F >Diff : 0 *F >CG3708|FBan0003708|CT12445|FBan0003708 last_updated:000321 *F >EG:BACR7A4.18 *F >Diff : A+ *F >CG3706|FBan0003706|CT12435|FBan0003706 last_updated:000321 *F >EG:BACR7A4.20 *F >Diff : 0 *F >CG11642|FBan0011642|CT36631|FBan0011642 last_updated:000321 *F >EG:BACR7A4.5 *F >Diff : 0 *F >CG3704|FBan0003704|CT12427|FBan0003704 last_updated:000321 *F >EG:BACR7A4.17 *F >Diff : 0 *F >CG3026|FBan0003026|CT10194|FBan0003026 last_updated:000321 *F >EG:BACR7A4.16 *F >Diff : 0 *F >CG3703|FBan0003703|CT12417|FBan0003703 last_updated:000321 *F >EG:BACR7A4.15 *F >Diff : A- *F >CG3699|FBan0003699|CT12411|FBan0003699 last_updated:000321 *F >EG:BACR7A4.14 *F >Diff : D- *F \# *F \#Comments: *F \# *F \#The \-COOH terminus (70-90 last a.a.) is completely different between the *F \#two genes. That is very strange, because EG:BACR7A4.14 is single-exon *F \#gene. Obviously, CG3699 has a splice site at some point which leads to *F \#different \-COOH sequence. *F \#However, ESTs AI293564 and AI294621 support the view that the exon *F \#predicted by EDGP is terminal. *F \# *F \#CONCLUSION: EG:BACR7A4.14 is more correct. *F \# *F >CG3690|FBan0003690|CT12383|FBan0003690 last_updated:000321 *F >EG:BACR7A4.13 *F >Diff : 0 *F >CG11638|FBan0011638|CT36619|FBan0011638 last_updated:000321 *F >EG:BACR7A4.12 *F >Diff : A++ *F >CG18091|FBan0018091|CT40606|FBan0018091 last_updated:000321 *F >EG:190E7.1 *F >Diff : 0 *F >CG3051|FBan0003051|CT10258|FBan0003051 last_updated:000321 *F >EG:132E8.2|FBgn0023169;SNF1A *F >Diff : 0 *F >CG3719|FBan0003719|CT12473|FBan0003719 last_updated:000321 *F >EG:132E8.3 *F >Diff : 0 *F >CG11448|FBan0011448|CT36231|FBan0011448 last_updated:000321 *F >EG:132E8.4 *F >Diff : 0 *F >CG18531|FBan0018531|CT42308|FBan0018531 last_updated:000321 *F >EG:BACN32G11.1 *F >Diff : 0 *F >CG3021|FBan0003021|CT10176|FBan0003021 last_updated:000321 *F >EG:BACR7A4.8 *F >Diff : C- *F >CG14630|FBan0014630|CT34388|FBan0014630 last_updated:000321 *F >EG:BACR7A4.9 *F >Diff : A- *F >CG14629|FBan0014629|CT34387|FBan0014629 last_updated:000321 *F >EG:103E12.2 *F >Diff : 0 *F >CG3655|FBan0003655|CT12291|FBan0003655 last_updated:000321 *F >EG:103E12.3 *F >Diff : 0 *F >CG11392|FBan0011392|CT31794|FBan0011392 last_updated:000321 *F >EG:BACR42I17.1 *F >Diff : B+ *F >CG11382|FBan0011382|CT31774|FBan0011382 last_updated:000321 *F >EG:BACR42I17.10 *F >Diff : 0 *F >CG11398|FBan0011398|CT31827|FBan0011398 last_updated:000321 *F >EG:BACR42I17.11 *F >Diff : 0 *F >CG14628|FBan0014628|CT34385|FBan0014628 last_updated:000321 *F >EG:BACR42I17.12 *F >Diff : 0 *F >CG11378|FBan0011378|CT31764|FBan0011378 last_updated:000321 *F >EG:BACR42I17.2 *F >Diff : 0 *F >CG11384|FBan0011384|CT31776|FBan0011384 last_updated:000321 *F >EG:BACR42I17.3 *F >Diff : 0 *F >CG11379|FBan0011379|CT31766|FBan0011379 last_updated:000321 *F >EG:BACR42I17.4 *F >Diff : 0 *F >CG14627|FBan0014627|CT34384|FBan0014627 last_updated:000321 *F >EG:BACR42I17.5 *F >Diff : A++ *F >CG14626|FBan0014626|CT34383|FBan0014626 last_updated:000321 *F >EG:BACR42I17.6 *F >Diff : A- *F >CG11380|FBan0011380|CT31768|FBan0011380 last_updated:000321 *F >EG:BACR42I17.7 *F >Diff : A++ *F >CG14625|FBan0014625|CT34382|FBan0014625 last_updated:000321 *F >EG:BACR42I17.8 *F >Diff : A+ *F >CG11491|FBan0011491|CT36317|FBan0011491 last_updated:000321 *F >EG:17A9.1_altrn_1|FBgn0000210;br /alternative *F >Diff : 0 *F >CG3791|FBan0003791|CT12681|FBan0003791 last_updated:000321 *F >EG:73D1.1 *F >Diff : 0 *F >CG14813|FBan0014813|CT34626|FBan0014813 last_updated:000321 *F >EG:63B12.10 *F >Diff : 0 *F >CG16903|FBan0016903|CT37506|FBan0016903 last_updated:000321 *F >EG:67A9.2 *F >Diff : C-- *F >CG3981|FBan0003981|CT13223|FBan0003981 last_updated:000321 *F >EG:67A9.1 *F >Diff : A- *F >CG3954|FBan0003954|CT37554|FBan0003954 last_updated:000321 *F >EG:BACN25G24.2|FBgn0000382;csw *F >Diff : 0 *F >CG3895|FBan0003895|CT12875|FBan0003895 last_updated:000321 *F >EG:BACN25G24.3|FBgn0004860;ph-d *F >Diff : A-- B+ C+ *F > \##### WARNING Known gene \! \##### *F >CG3540|FBan0003540|CT11900|FBan0003540 last_updated:000321 *F >EG:152A3.2 *F >Diff : 0 *F >CG10755|FBan0010755|CT30144|FBan0010755 last_updated:000321 *F >EG:152A3.6|FBgn0015036;Cyp4e4 *F >Diff : 0 *F >CG18031|FBan0018031|CT40356|FBan0018031 last_updated:000321 *F >EG:103B4.2 *F \#>Diff : B C- *F >Diff : D *F \# *F \#Comments: *F \# *F \# B : differences on internal exon(s) are due to different prediction *F \# EDGP followed Genefinder; Genscan (and \-presumambly Genie- supports *F \# one more exon. Similarity hits from both SW and TrEMBL though, *F \# favour EDGP's (a.k.a. Genefinder's) prediction. (although the hits *F \# are not very strong) *F \# C : differences in \-COOH terminus are probably due to different *F \# prediction methods used. There is no supporting evidence for any of *F \# the gene structures there. *F \# *F \#CONCLUSION: Ambigious or EG:103B4.2 is more correct. *F \# *F >CG3218|FBan0003218|CT10801|FBan0003218 last_updated:000321 *F >EG:30B8.5|FBgn0000810;fs(1)K10 *F >Diff : 0 *F >CG14050|FBan0014050|CT33609|FBan0014050 last_updated:000321 *F >EG:BACH48C10.1 *F >Diff : 0 *F >CG2854|FBan0002854|CT9762|FBan0002854 last_updated:000321 *F >EG:BACH48C10.2 *F >Diff : C- *F >CG14048|FBan0014048|CT33607|FBan0014048 last_updated:000321 *F >EG:BACH48C10.6 *F >Diff : 0 *F >CG2841|FBan0002841|CT9712|FBan0002841 last_updated:000321 *F >EG:BACH48C10.5 *F >Diff : A+ *F >CG14047|FBan0014047|CT33606|FBan0014047 last_updated:000321 *F >EG:BACH48C10.4 *F \#>Diff : A-- B- B+ B+ C- *F >Diff : D *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are probably due to incomplete analysis *F \# of EDGP. Gene EG:BACH48C10.4 is located at the end of BAC H48C10 *F \# (reverse orientation) and is probably continuing on BAC H7M4. In *F \# this case gene EG:BACH7M4.1 should match the NH2- terminus of *F \# CG14047. *F \# B : differences on internal exons should be due to different prediction *F \# algorithms used. Although, in this case, EDGP followed the Genscan *F \# prediction (which should be similar to Genie that Celera used), it is *F \# possible that the two software programs are utilising different exons. *F \# C : differences on the \-COOH are probably due to different prediction *F \# methods used. It applies the same as in (B). *F \# *F \# (There is no supportive evidence to favour one or the other prediction.) *F \# *F >CG13756|FBan0013756|CT33235|FBan0013756 last_updated:000321 *F >EG:BACH59J11.2 *F >Diff : B+ *F >CG14045|FBan0014045|CT33604|FBan0014045 last_updated:000321 *F >EG:BACH7M4.1 *F >Diff : A-- *F >CG14045|FBan0014045|CT33604|FBan0014045 last_updated:000321 *F >EG:BACH7M4.2 *F >Diff : A- C- *F >CG12496|FBan0012496|CT33234|FBan0012496 last_updated:000321 *F >EG:BACH7M4.4 *F >Diff : C- *F >CG12497|FBan0012497|CT33237|FBan0012497 last_updated:000321 *F >EG:BACR25B3.2 *F >Diff : A+ B+ *F >CG13758|FBan0013758|CT33238|FBan0013758 last_updated:000321 *F >EG:BACR25B3.3 *F \#>Diff : A+ B C+ *F >Diff : D *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are due to different prediction methods *F \# used. Although, in this case, EDGP followed the Genscan prediction *F \# (which should be similar to Genie that Celera used), it is possible *F \# that the two software programs are utilising different starting *F \# exons. There is no other evidence to favour one or the other *F \# version. *F \# B : differences on internal exons should be due to frameshift errors *F \# (most probably). *F \# C : differences in \-COOH terminus are due to different prediction methods *F \# used. Although, in this case, EDGP followed the Genscan prediction *F \# (which should be similar to Genie that Celera used), it is possible *F \# that the two software programs are utilising different end exons. In *F \# fact, the last exon that Genscan/EDGP predicts (not present in *F \# Genefinder prediction) is some 1.5 kb downstream of the previous one. *F \# There is no supportive evidence to favour one or the other prediction. *F \# *F \#CONCLUSION: Ambigious. *F \# *F >CG13759|FBan0013759|CT33239|FBan0013759 last_updated:000321 *F >EG:BACR25B3.5 *F >Diff : B+ *F >CG13760|FBan0013760|CT33241|FBan0013760 last_updated:000321 *F >EG:BACR25B3.6 *F >Diff : A-- *F >CG17437|FBan0017437|CT33240|FBan0017437 last_updated:000321 *F >EG:BACR25B3.7 *F >Diff : 0 *F >CG13761|FBan0013761|CT33242|FBan0013761 last_updated:000321 *F >EG:BACR7C10.4 *F >Diff : C+ *F >CG10742|FBan0010742|CT30103|FBan0010742 last_updated:000321 *F >EG:BACR7C10.6 *F >Diff : 0 (6/302) *F >CG9904|FBan0009904|CT27892|FBan0009904 last_updated:000321 *F >EG:BACR7C10.1 *F >Diff : 0 *F >CG13762|FBan0013762|CT33244|FBan0013762 last_updated:000321 *F >EG:BACR7C10.7 *F >Diff : B- *F >CG10260|FBan0010260|CT28483|FBan0010260 last_updated:000321 *F >EG:BACR7C10.2 *F >Diff : A+ B- B+ (over 2160 a.a.) *F >Diff : D *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are minor and involve different starting *F \# ATG selection (2 a.a. apart). *F \# B : the initial differences on internal exon should be due to different *F \# prediction algorithms used. EDGP follows Genefinder prediction, *F \# which in this case seems more trustworthy (Genscan predicts a tiny *F \# intron in the region). Both programs however, agree on the 'main *F \# body' of the prediction, i.e. region starting at 'ADKLCG...'. Thus, *F \# the absence of one region in CG10260 is unexplainable there. *F \# *F \#CONCLUSION: Ambigious or EG:BACR7C10.2 is more correct. *F \# *F >CG12498|FBan0012498|CT33246|FBan0012498 last_updated:000321 *F >EG:BACR43E12.1 *F >Diff : 0 *F >CG14416|FBan0014416|CT34073|FBan0014416 last_updated:000321 *F >EG:BACR43E12.7 *F >Diff : 0 *F >CG3588|FBan0003588|CT11992|FBan0003588 last_updated:000321 *F >EG:100G7.6 *F >Diff : A-- C+ *F >CG14424|FBan0014424|CT34081|FBan0014424 last_updated:000321 *F >EG:100G7.5 *F >Diff : 0 *F >CG7981|FBan0007981|CT23996|FBan0007981 last_updated:000321 *F >EG:BACR25B3.11 *F >Diff : D (identity starts at 3285 a.a. of CG) *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are due to different prediction methods *F \# used. EDGP follows the Genfinder prediction, whereas CG7981 seems to *F \# be similar to the Genscan one. In this case the NH2- terminus of the *F \# CG7981 should be EG:BACR25B3.10. *F \# C : differences in \-COOH terminus are also due to different prediction *F \# methods used. Genscan, suggests a highly unprobable 'long' gene *F \# structure (big introns, small exons), probably derived from its *F \# 'human' training set. I don't know whether Genie (the primary used *F \# Celera program) was trained on similar data set. *F \# *F \#CONCLUSION: Ambigious. *F \# *F >CG7981|FBan0007981|CT23996|FBan0007981 last_updated:000321 *F >EG:BACR25B3.10 *F >Diff : D (identity starts at 2448 a.a. of CG) *F \# *F >CG7981|FBan0007981|CT23996|FBan0007981 last_updated:000321 *F >EG:BACR25B3.1 *F >Diff : A++ C- *F \# *F \#================================================================== *F \#GENERAL Comments for EG:BACR25B3.1, EG:BACR25B3.10, EG:BACR25B3.11 *F \#================================================================== *F \# *F \#This was one of the most difficult regions to annotate (PVB did it during *F \#his visit to Cambridge, Feb. 2000). It seems like it has undergone *F \#multiple duplications. Genscan, predicts one very very very long gene *F \#there (biased from its Human training set, i suppose); so does Celera. *F \#(based on Genie; from which organism Genie's training set was derived?) *F \#This is 'the longest Drosophila gene' that is described on the paper. *F \#PVB has split the gene into three 'smaller' ones and XDrosDB comments say *F \#that 'TVB-990205: i'm not sure about this gene; ...'. *F \#However, based on similarity matches (considering the ranges of the hits), *F \#we can safely say that 'the longest Drosophila gene' does NOT exist (not *F \#as such, anyway). *F \# *F \#CONCLUSION: EG:BACR25B3.1, EG:BACR25B3.10, EG:BACR25B3.11 should be more *F correct. *F \# *F >CG12467|FBan0012467|CT32681|FBan0012467 last_updated:000321 *F >EG:34F3.1 *F \#>Diff : A++ C *F >Diff : D (CG extends 800 a.a. further down) *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are due to different selection of *F \# starting ATG. CG12467 chooses an in-frame ATG 39 a.a. downstream of *F \# the one of EG:34F3.1, for no obvious reason. *F \# C : differences in \-COOH terminus are probably due to different *F \# prediction methods used. EDGP follows the Genefinder prediction; *F \# Celera, follows Genie's one. I'm expecting that the 'missing' 800 *F \# a.a. are part of EG:34F3.2 gebe (which is downstream of EG:34F3.1). *F \# *F \# *F \#Mel_Comments: *F \# *F \#Takis you suggested that EG:34F3.1 and 34F3.2 equal CG12467 and I agree. *F \# *F \#34F3.1 starts at \+40 of the CG gene *F \# it has a perfect match until aa position 524 *F \# *F \#34F3.2 starts at \+525 of the CG gene *F \# one gap is present and the C terminus is messed up. *F \# *F \#You have suggested that the last two exons of 34F3.2 should form anothe *F \#CG gene but I have been unable to find another matching CG gene for *F \#their aa sequence. *F \# *F \#CONCLUSION: Ambigious or EG:34F3.1 is more correct. *F \# *F >EG:87B1.5|FBgn0004861_ph-p *F >EG:BACN25G24.3|FBgn0004860_ph-d *F >Diff : D *F > \##### WARNING Known gene!##### *F \# *F \#Comments: *F \# *F \# It seems like Celera has a mis-assembly on the genomic sequence, *F \# resulting in incorrect model. The mis-assembly is probably due to a *F \# genomic duplication that resulted into ph-p and ph-d. *F \# *F >CG4857|FBan0004857|CT15601|FBan0004857 last_updated:000321 *F >EG:EG0007.12; CAA21828.1 *F >Diff : D *F \# *F \#Comments: *F \# *F \#EG:EG0007.12 is a mere Genefinder prediction, although with very very high *F \#score (137.81, when our cutoff is 50 and BDGP's cutoff was 20). ESTs *F \#support practically the 2nd and 3rd exon; but there is no other evidence *F \#to favour EDGP's or CG's version. *F \# *F \#CONCLUSION: Ambigious. *F \# *F >CG4857|FBan0004857|CT15601|FBan0004857 last_updated:000321 *F >EG:EG0007.4; CAA21827.1 *F \#>Diff : B- C *F >Diff : D (CG extends 1000 a.a. further down) *F \# *F \#Comments: *F \# *F \# C : differences in \-COOH terminus are probably due to different *F \# prediction methods used. EDGP follows the Genefinder prediction; *F \# Celera, follows Genie's one. *F \# *F \#CONCLUSION: Ambigious. *F \# *F >CG2766|FBan0002766|CT9405|FBan0002766 last_updated:000321 *F >CG2766|FBan0002766|CT9405|FBan0002766 last_updated:000321 *F >EG:BACN33B1.2 *F \#>Diff : A+ *F >Diff : D *F \# *F \#Comments: *F \#The first 61 a.a. of EG:BACN33B1.2 are matching the first 61 a.a. of *F \#CG2766, whereas the rest of the gene matches CG2716. *F \# *F \#I checked the genomic structure and all data. Gene EG:BACN33B1.2 is a *F \#mere Genscan prediction. Genefinder predicts two genes (probably the *F \#equivalent of CG2766 and CG2766). However, EST AI512291, which covers the *F \#first 4 exons of the gene, supports EG:BACN33B1.2 over CG2766 \+ CG2766. *F \# *F \#CONCLUSION: EG:BACN33B1.2 is more correct. *F \# *F >CG2716|FBan0002716|CT9237|FBan0002716 last_updated:000321 *F >EG:BACN33B1.2 *F >Diff : D *F \# *F \# *F \#Comments: *F \# *F \#The first 61 a.a. of EG:BACN33B1.2 matches exactly the corresponding *F \#CG2766 region. The whole gene matches CT9237 (from CT9237:140 onwards). *F \#EG:BACN33B1.2 is a mere Genscan prediction supported by multiple ESTs on *F \#all but its last exon. *F \#I don't know where CG2716 comes from or why CG2716 is different than *F \#CT9237. *F \# *F \#CONCLUSION: Ambigious or EG:BACN33B1.2 is more correct. *F \# *F \# *F \#General Comments: *F \#Based on EST hit(s), genes CG2766 \+ CG2716 should concatanate to *F \#EG:BACN33B1.2. *F \# *F >CG3713|FBan0003713|CT12463|FBan0003713 last_updated:000321 *F >EG:BACR7A4.2 *F >Diff : 0 (suspicious... only 83 a.a. long) *F >CG4015|FBan0004015|CT41858|FBan0004015 last_updated:000321 *F >EG:140G11.3 *F >Diff : 0 *F >CG3526|FBan0003526|CT11880|FBan0003526 last_updated:000321 *F >EG:BACR43E12.4 *F >Diff : A++ *F >CG3939|FBan0003939|CT13113|FBan0003939 last_updated:000321 *F >EG:140G11.5 *F >Diff : A+ *F >CG14265|FBan0014265|CT33887|FBan0014265 last_updated:000321 *F >EG:96G10.8 *F >Diff : 0 *F >CG18166|FBan0018166|CT40990|FBan0018166 last_updated:000321 *F >EG:171D11.6 *F >Diff : D *F \# *F \#Comments: *F \# *F \#Completely different gene structure. *F \#EG:171D11.6 follows the 'consensus' prediction of Genefinder and Genscan *F \#(practically identical except the last exon(s)); and is supported by *F \#multiple ESTs. *F \#One thing that might misled the Celera annotators is that this region/gene *F \#seems to be duplicated (as shown by the multiple hits of some of the ESTs *F \#(e.g. AA438842). *F \# *F \#CONCLUSION: EG:171D11.6 is more correct. *F \# *F >CG18273|FBan0018273|CT41446|FBan0018273 last_updated:000321 *F >EG:171D11.6 *F >Diff : B+ C++ *F \# *F \#General Comments: *F \#Both genes CG18166 & CG18273 match gene EG:171D11.6, but in a 'weird' *F \#way. EG:171D11.6 follows both Genefinder and Genscan predictions, as well *F \#as the corresponding EST matches. If there is not a mis-assembly case, i *F \#don't know how to explain it... *F \# *F \#Mel_Comments: *F \#The two clustal analysis of this gene which you looked at were incorrect. *F \#I can only assume that Michael tried clustals on these two CG genes, *F \#CG18166 and CG18273, before he found the correct matching CG gene which is *F \#CG13372. *F \# *F \#This is matched perfectly until aa positon 1006, gapped for 50 aa and *F \#finally the CG gene misses the last 250aa of the EG gene. *F \# *F \#CONCLUSION: EG:171D11.6 is more correct. *F \# *F >CG18503|FBan0018503|CT42214|FBan0018503 last_updated:000321 *F >EG:171D11.3_altrn_1|FBgn0004648;svr /alternative *F >Diff : D *F \# *F \#Comments: *F \#This is the ALTERNATIVE transcript/protein, based on EST and protein *F \#hits. The two transcripts differ on their 5' exon(s). CG18503 attaches *F \#the 5'-most exon in a completely different gene. *F \#PVB believes that EG:171D11.3_altrn_1 is the correct gene structure for *F \#the alternatively spliced EG:171D11.3 gene. *F \# *F \#CONCLUSION: EG:171D11.3_altrn_1 (svr alternative) is correct. *F \# *F >CG14624|FBan0014624|CT34381|FBan0014624 last_updated:000321 *F >EG:BACR42I17.9 *F \#>Diff : A++ *F >Diff : D (CG extends 460 a.a. further up) *F \# *F \#Comments: *F \# *F \# A : differences in NH2- terminus are due to different prediction methods *F \# used. EDGP follows the Genefinder prediction. Probably, the first *F \# 460 a.a. are part of EG:BACR42I17.8 gene. *F \# *F \# *F \#Mel_Comments: *F \# *F \#There are two clustals of this gene. *F \#The clustal of 42I17.9 with CG11381 begins at \+25 of the CG gene \- if you *F \#look closely you notice that the first 6 aa of the EG gene match the *F \#begining of the CG gene. This then has a perfent match until position 446 *F \#and the CG gene misses the next ~260aa. *F \#In the clustal of 42I17.9 with CG14624 the CG gene begins at \+463 and is *F \#matched perfectly until the end. *F \# *F \#CONCLUSION: Ambigious. *F \# *F >CG11381|FBan0011381|CT31772|FBan0011381 last_updated:000321 *F >EG:BACR42I17.9 *F >Diff : A-- C+ *F >CG18089|FBan0018089|CT40590|FBan0018089 last_updated:000321 *F >EG:100G7.1|FBgn0014096;anon-3Ca *F >Diff : 0 (suspicious... only 56 a.a. long) *F >CG2945|FBan0002945|CT9961|FBan0002945 last_updated:000321 *F >EG:BACR37P7.3|FBgn0000316;cin *F >Diff : 0 *F >CG3114|FBan0003114|CT10322|FBan0003114 last_updated:000321 *F >EG:BACR37P7.7|FBgn0005427;ewg *F >Diff : B+ *F >CG12470|FBan0012470|CT32706|FBan0012470 last_updated:000321 *F >EG:BACR37P7.5 *F >Diff : 0 *F > \##### WARNING Cant find translation for EG:BACR37P7.5!##### *F \# *F \#Comments: *F \#Translation found and is 100% identical to CG12470. *F \# *F >CG3777|FBan0003777|CT12604|FBan0003777 last_updated:000321 *F >EG:125H10.1 *F >Diff : 0 *F >CG3757|FBan0003757|CT12485|FBan0003757 last_updated:000321 *F >EG:125H10.2|FBgn0004034;y *F >Diff : 0 *F >CG3796|FBan0003796|CT12661|FBan0003796 last_updated:000321 *F >EG:125H10.3|FBgn0000022;ac *F >Diff : 0 *F >CG3827|FBan0003827|CT12777|FBan0003827 last_updated:000321 *F >EG:198A6.1|FBgn0004170;sc *F >Diff : 0 *F >CG3839|FBan0003839|CT12815|FBan0003839 last_updated:000321 *F >EG:198A6.2|FBgn0002561;l(1)sc *F >Diff : 0 *F >CG13374|FBan0013374|CT32705|FBan0013374 last_updated:000321 *F >EG:EG0001.1|FBgn0011822;pcl *F >Diff : 0 *F >CG3258|FBan0003258|CT10959|FBan0003258 last_updated:000321 *F >EG:165H7.2|FBgn0000137;ase *F >Diff : 0 *F >CG3972|FBan0003972|CT13187|FBan0003972 last_updated:000321 *F >EG:165H7.1|FBgn0011757;ASC-T1 *F >Diff : 0 *F >CG3923|FBan0003923|CT13059|FBan0003923 last_updated:000321 *F >EG:165H7.3 *F \#>Diff : A+ C+ (probably different selection of starting exon) *F >Diff : D+ *F \# *F \#Comments: *F \# *F \#Only a.a. EG:165H7.3:40-190 match CG3923. *F \#EG:165H7.3 is the 'consensus' prediction of Genefinder and Genscan (aggree *F \#on all but one exon). I had added one more NH2- exon, following multiple *F \#EST hits (e.g. AA439864). *F \#I don't know where the rest of CG3923 comes from or why Celera missed this *F \#gene. *F \# *F \#CONCLUSION: EG:165H7.3 is correct. *F \# *F >CG13372|FBan0013372|CT32701|FBan0013372 last_updated:000321 *F >EG:171D11.6 *F >Diff : B+ C+ *F >CG3156|FBan0003156|CT10534|FBan0003156 last_updated:000321 *F >EG:171D11.2 *F >Diff : A- *F >CG17896|FBan0017896|CT39849|FBan0017896 last_updated:000321 *F >EG:171D11.1 *F >Diff : 0 *F >CG17896|FBan0017896|CT39849|FBan0017896 last_updated:000321 *F >EG:171D11.1_altrn_1 /alternative *F >Diff : A- *F >CG17778|FBan0017778|CT13856|FBan0017778 last_updated:000321 *F >EG:171D11.5 *F >Diff : 0 *F >CG4122|FBan0004122|CT41466|FBan0004122 last_updated:000321 *F >EG:171D11.3|FBgn0004648;svr *F \#>Diff : A+ B+ D *F >Diff : D *F \# *F \#Comments: *F \# *F \#Gene EG:171D11.3 is KNOWN (FBgn0004648;svr) and is (or should be) *F \#identical to U29591; apart from the last exon, where the original cloning *F \#should had contained a frameshift. *F \#I don't know why CG4122 doesn't match. *F \# *F \#CONCLUSION: EG:171D11.3 is correct. *F \# *F >CG18503|FBan0018503|CT42214|FBan0018503 last_updated:000321 *F >EG:171D11.3_altrn_1|FBgn0004648;svr /alternative, mismatched *F >Diff : D *F \# *F \#General Comments: *F \#Only the first 152 a.a. of EG:171D11.3a match the CG18503. *F \# *F \#This is the alternative svr gene, which corresponds to U29592. *F \# *F \#CONCLUSION: EG:171D11.3a is correct. *F \# *F >CG4262|FBan0004262|CT13920|FBan0004262 last_updated:000321 *F >EG:65F1.2|FBgn0000570;elav *F >Diff : 0 *F >CG4293|FBan0004293|CT14025|FBan0004293 last_updated:000321 *F >EG:65F1.1 *F >Diff : 0 *F >CG7727|FBan0007727|CT23451|FBan0007727 last_updated:000321 *F >EG:65F1.5|FBgn0000108;Appl *F >Diff : A+ *F >CG6172|FBan0006172|CT19338|FBan0006172 last_updated:000321 *F >EG:118B3.1|FBgn0003986;vnd *F >Diff : 0 *F >CG13366|FBan0013366|CT32693|FBan0013366 last_updated:000321 *F >EG:118B3.2 *F >Diff : 0 *F >CG17828|FBan0017828|CT39571|FBan0017828 last_updated:000321 *F >EG:115C2.5 *F >Diff : 0 *F >CG13369|FBan0013369|CT32698|FBan0013369 last_updated:000321 *F >EG:115C2.1 *F >Diff : 0 *F >CG18451|FBan0018451|CT32691|FBan0018451 last_updated:000321 *F >EG:115C2.12 *F >Diff : 0 *F \# *F \#WARNING: Only 64 a.a. long!? *F >CG7622|FBan0007622|CT23257|FBan0007622 last_updated:000321 *F >EG:115C2.7|FBgn0002579;RpL36 *F >Diff : 0 *F >CG6189|FBan0006189|CT19398|FBan0006189 last_updated:000321 *F >EG:115C2.2|FBgn0001341;l(1)1Bi *F >Diff : 0 *F >CG7486|FBan0007486|CT22949|FBan0007486 last_updated:000321 *F >EG:115C2.9|FBgn0020381;Dredd *F >Diff : B- *F >CG6222|FBan0006222|CT19476|FBan0006222 last_updated:000321 *F >EG:115C2.3|FBgn0003575;su(s) *F >Diff : 0 *F >CG13367|FBan0013367|CT32696|FBan0013367 last_updated:000321 *F >EG:115C2.8 *F >Diff : A- *F >CG16982|FBan0016982|CT32695|FBan0016982 last_updated:000321 *F >EG:115C2.11 *F >Diff : B+ *F >CG13363|FBan0013363|CT32690|FBan0013363 last_updated:000321 *F >EG:115C2.10 *F >Diff : C- *F >CG16983|FBan0016983|CT32694|FBan0016983 last_updated:000321 *F >EG:115C2.4 *F >Diff : 0 *F >CG5227|FBan0005227|CT16627|FBan0005227 last_updated:000321 *F >EG:BACR19J1.1|FBgn0021764;sdk *F >Diff : B+ *F >CG7434|FBan0007434|CT22861|FBan0007434 last_updated:000321 *F >EG:BACR19J1.4|FBgn0015288;RpL22 *F >Diff : 0 *F >CG7359|FBan0007359|CT22657|FBan0007359 last_updated:000321 *F >EG:34F3.8 *F >Diff : 0 *F >CG13358|FBan0013358|CT32683|FBan0013358 last_updated:000321 *F >EG:34F3.1059 *F >Diff : A+ *F >CG13359|FBan0013359|CT32684|FBan0013359 last_updated:000321 *F >EG:34F3.9 *F >Diff : B- *F >CG7413|FBan0007413|CT22763|FBan0007413 last_updated:000321 *F >EG:34F3.3|FBgn0015799;Rbf *F >Diff : 0 *F >CG16989|FBan0016989|CT32688|FBan0016989 last_updated:000321 *F >EG:34F3.4 *F >Diff : 0 *F >CG13360|FBan0013360|CT32685|FBan0013360 last_updated:000321 *F >EG:34F3.5 *F >Diff : 0 *F >CG12311|FBan0012311|CT21087|FBan0012311 last_updated:000321 *F >EG:34F3.7 *F >Diff : A- C- *F >CG3658|FBan0003658|CT12303|FBan0003658 last_updated:000321 *F >EG:BACR7A4.11|FBgn0026143;CDC45L *F >Diff : 0 *F >CG3019|FBan0003019|CT10162|FBan0003019 last_updated:000321 *F >EG:BACR7A4.10|FBgn0003638; su(wa) (/partial; this is the 5' terminus) *F >Diff : B- *F \#### WARNING: check the 3' terminus too.... *F \# identity up to EG:BACR7A4.10 1-224 *F >CG3638|FBan0003638|CT12157|FBan0003638 last_updated:000321 *F >EG:33C11.3 *F >Diff : A+ C-- *F >CG11403|FBan0011403|CT31837|FBan0011403 last_updated:000321 *F >EG:33C11.2 *F >Diff : 0 *F >CG11405|FBan0011405|CT31841|FBan0011405 last_updated:000321 *F >EG:33C11.1 *F >Diff : 0 *F >CG11408|FBan0011408|CT31847|FBan0011408 last_updated:000321 *F >EG:114D9.1 *F >Diff : 0 *F >CG14622|FBan0014622|CT34379|FBan0014622 last_updated:000321 *F >EG:114D9.2 *F >Diff : A-- *F >CG11411|FBan0011411|CT31859|FBan0011411 last_updated:000321 *F >EG:8D8.1 *F >Diff : 0 *F >CG11409|FBan0011409|CT31851|FBan0011409 last_updated:000321 *F >EG:8D8.2 *F >Diff : 0 *F >CG11412|FBan0011412|CT31863|FBan0011412 last_updated:000321 *F >EG:8D8.6 *F >Diff : B+ *F >CG11418|FBan0011418|CT31875|FBan0011418 last_updated:000321 *F >EG:8D8.8 *F >Diff : 0 *F >CG11415|FBan0011415|CT31869|FBan0011415 last_updated:000321 *F >EG:8D8.7 *F >Diff : 0 *F >CG12773|FBan0012773|CT31881|FBan0012773 last_updated:000321 *F >EG:8D8.3 *F >Diff : 0 *F >EG:8D8.4 *F >CG11417|FBan0011417|CT31873|FBan0011417 last_updated:000321 *F >Diff : 0 *F >CG11420|FBan0011420|CT31887|FBan0011420 last_updated:000321 *F >EG:8D8.5 *F >Diff : 0 *F >CG3056|FBan0003056|CT10284|FBan0003056 last_updated:000321 *F >EG:132E8.1 *F >Diff : 0 *F >CG3064|FBan0003064|CT10298|FBan0003064 last_updated:000321 *F >EG:49E4.1 *F \#>Diff : A- B- C+++ *F >Diff : D *F \# *F \#Comments: *F \# *F \#Gene EG:49E4.1 is a mere Genefinder prediction with very high score *F \#(404.95). Genscan prediction is practically the same, except it has a *F \#couple of additional NH2- terminus exons. Exons 3, 4, 5, 6, 8 and 9 are *F \#supported further by protein similarity hits (e.g. MAPB_HUMAN). *F \#I don't know where CG3064 comes from. *F \# *F \#CONCLUSION: EG:49E4.1 is more correct. *F \# *F >CG14785|FBan0014785|CT34595|FBan0014785 last_updated:000321 *F >EG:BACN32G11.2 *F >Diff : 0 *F >CG14786|FBan0014786|CT34596|FBan0014786 last_updated:000321 *F >EG:BACN32G11.3 *F >Diff : 0 *F >CG14787|FBan0014787|CT34597|FBan0014787 last_updated:000321 *F >EG:BACN32G11.4 *F >Diff : A- *F >CG14788|FBan0014788|CT34598|FBan0014788 last_updated:000321 *F >EG:BACN32G11.5 *F >Diff : 0 *F >CG14789|FBan0014789|CT34599|FBan0014789 last_updated:000321 *F >EG:BACN32G11.6 *F >Diff : A- *F >CG14777|FBan0014777|CT34587|FBan0014777 last_updated:000321 *F >EG:80H7.10 *F >Diff : 0 *F \##### WARNING: No corresponding CG to EG:80H7.1 \! *F \##### WARNING: Can't find translation of EG:80H7.2 \! *F >CG14780|FBan0014780|CT34590|FBan0014780 last_updated:000321 *F >EG:80H7.3 *F >Diff : 0 *F >CG14791|FBan0014791|CT34601|FBan0014791 last_updated:000321 *F >EG:80H7.4 *F >Diff : B- *F >CG14781|FBan0014781|CT34591|FBan0014781 last_updated:000321 *F >EG:80H7.11 *F >Diff : B+ *F >CG14782|FBan0014782|CT34592|FBan0014782 last_updated:000321 *F >EG:80H7.5 *F >Diff : 0 *F >CG14792|FBan0014792|CT34602|FBan0014792 last_updated:000321 *F >EG:80H7.6|FBgn0003517;sta *F \#>Diff : A- B C+ *F >Diff : A- B C+ *F >CG14793|FBan0014793|CT34603|FBan0014793 last_updated:000321 *F >EG:80H7.7 *F >Diff : D *F \# *F \#Comments: *F \# *F \#Differences in COOH- terminus are probably due to different prediction *F \#methods used. In this case, EG:80H7.7 follows the Genefinder prediction *F \#and protein similarity hits. *F \#Genscan prediction extends downstream to include two more exons, which in *F \#fact correspond to gene sta (or EG:80H7.6). *F \#If this is true, the \-COOH terminus of CG14793 should align with 80H7.6. *F \# *F \#CONCLUSION: EG:80H7.7 is more correct. *F \# *F >CG14795|FBan0014795|CT34605|FBan0014795 last_updated:000321 *F >EG:196F3.3 *F >Diff : A+ *F >CG14783|FBan0014783|CT34593|FBan0014783 last_updated:000321 *F >EG:196F3.2 *F >Diff : C+ *F >CG14796|FBan0014796|CT34607|FBan0014796 last_updated:000321 *F >EG:56G7.1 *F >Diff : 0 *F >CG11491|FBan0011491|CT36317|FBan0011491 last_updated:000321 *F >CG11509|FBan0011509|CT36379|FBan0011509 last_updated:000321 *F >CG11511|FBan0011511|CT34608|FBan0011511 last_updated:000321 *F >CG11514|FBan0011514|CT36387|FBan0011514 last_updated:000321 *F >EG:17A9.1|FBgn0000210;br *F >Diff : B-- *F \##### Note: There are alternatively spliced products for br. *F >CG3093|FBan0003093|CT10396|FBan0003093 last_updated:000321 *F >EG:171E4.1|FBgn0000482;dor *F >Diff : 0 *F >CG3740|FBan0003740|CT12509|FBan0003740 last_updated:000321 *F >EG:171E4.4 *F >Diff : D *F \# *F \#Comments: *F \# *F \#Only the first 55 a.a. of the two genes match. *F \# *F \#EG:171E4.4 is a small two-exon gene, located betwen EG:171E4.1 (dor) and *F \#EG:171E4.2 (on their opposite strand). It is a modified prediciton of *F \#both Genefinder and Genscan programs; but both give relatively low score. *F \#The reason it was reported is that matches EST AA142192 (on the correct *F \#orientation). *F \#I don't know where \-COOH terminus of gene CG3740 is located though. *F \# *F \#CONCLUSION: EG:171E4.4 is more correct. *F \# *F >CG3095|FBan0003095|CT10406|FBan0003095 last_updated:000321 *F >EG:171E4.2 *F >Diff : A+ C+ *F >CG3737|FBan0003737|CT12505|FBan0003737 last_updated:000321 *F >EG:171E4.3 *F >Diff : 0 *F >CG3100|FBan0003100|CT10412|FBan0003100 last_updated:000321 *F >EG:9D2.1|FBgn0024897;b6 *F >Diff : 0 *F >CG3783|FBan0003783|CT12641|FBan0003783 last_updated:000321 *F >EG:9D2.2 *F >Diff : D *F \##### Note: Only one exon of EG:9D2.2 matches CG3783! *F \# *F \#Comments: *F \# *F \#EG:9D2.2 is the intact Genscan prediction. The first 4 exons match the *F \#genomic sequence for gene a6 (Y16065), but i decided to leave them in *F \#because: (a) the ORF of a6 gene 700-800 bp upstream and (b) exon 4 matches *F \#EST AI403894, which further extends to exons 5 and 6. *F \#Maybe Celera didn't include the first 4 exons, because of their overlap *F \#with Y16065 *F \# *F \#CONCLUSION: EG:9D2.2 is more correct. *F \# *F >CG3771|FBan0003771|CT12608|FBan0003771 last_updated:000321 *F >EG:9D2.3|FBgn0023130;a6 *F >Diff : C- *F >CG3795|FBan0003795|CT12705|FBan0003795 last_updated:000321 *F >EG:9D2.4 *F >Diff : 0 *F >CG14808|FBan0014808|CT34621|FBan0014808 last_updated:000321 *F >EG:4F1.1 *F >Diff : 0 *F >CG12598|FBan0012598|CT34611|FBan0012598 last_updated:000321 *F >EG:BACN35H14.1 *F >Diff : A+ *F >CG17968|FBan0017968|CT40057|FBan0017968 last_updated:000321 *F >EG:137E7.1 *F >Diff : 0 *F \##### Note: Only 64 a.a. long! *F >CG14801|FBan0014801|CT34614|FBan0014801 last_updated:000321 *F >EG:131F2.2 *F >Diff : A- *F >CG14812|FBan0014812|CT34625|FBan0014812 last_updated:000321 *F >EG:131F2.3 *F >Diff : 0 *F >CG14814|FBan0014814|CT34627|FBan0014814 last_updated:000321 *F >EG:63B12.6 *F >Diff : A- *F >CG14802|FBan0014802|CT34615|FBan0014802 last_updated:000321 *F >EG:63B12.13 *F >Diff : 0 *F >CG14815|FBan0014815|CT34628|FBan0014815 last_updated:000321 *F >EG:63B12.5 *F >Diff : 0 *F >CG14803|FBan0014803|CT34616|FBan0014803 last_updated:000321 *F >EG:63B12.9 *F >Diff : B+ *F >CG14816|FBan0014816|CT34629|FBan0014816 last_updated:000321 *F >EG:63B12.4 *F >Diff : 0 *F >CG14804|FBan0014804|CT34617|FBan0014804 last_updated:000321 *F >EG:63B12.8 *F >Diff : 0 *F >CG14817|FBan0014817|CT34630|FBan0014817 last_updated:000321 *F >EG:63B12.11 *F >Diff : 0 *F >CG14805|FBan0014805|CT34618|FBan0014805 last_updated:000321 *F >EG:63B12.7 *F >Diff : B+ *F >CG14818|FBan0014818|CT34631|FBan0014818 last_updated:000321 *F >EG:63B12.12 *F >Diff : 0 *F >CG3848|FBan0003848|CT12853|FBan0003848 last_updated:000321 *F >EG:63B12.3 *F >Diff : B++ *F >CG3109|FBan0003109|CT10436|FBan0003109 last_updated:000321 *F >EG:63B12.2 *F >Diff : B+ *F >CG11579|FBan0011579|CT12773|FBan0011579 last_updated:000321 *F >EG:86E4.6|FBgn0000117;arm *F >Diff : A+ *F >CG3810|FBan0003810|CT36539|FBan0003810 last_updated:000321 *F >EG:86E4.2 *F >Diff : C+ *F >CG17766|FBan0017766|CT39345|FBan0017766 last_updated:000321 *F >EG:86E4.3 *F >Diff : A- *F >CG3480|FBan0003480|CT11715|FBan0003480 last_updated:000321 *F >EG:86E4.4 *F >Diff : 0 *F >CG3806|FBan0003806|CT12735|FBan0003806 last_updated:000321 *F >EG:86E4.1 *F >Diff : 0 *F >CG3573|FBan0003573|CT11908|FBan0003573 last_updated:000321 *F >EG:86E4.5 *F >Diff : 0 *F >CG11596|FBan0011596|CT12035|FBan0011596 last_updated:000321 *F >EG:39E1.1 *F >Diff : 0 *F >CG3857|FBan0003857|CT12883|FBan0003857 last_updated:000321 *F >EG:39E1.3 *F >Diff : 0 *F >CG3587|FBan0003587|CT12059|FBan0003587 last_updated:000321 *F >EG:39E1.2 *F >Diff : 0 *F >CG3600|FBan0003600|CT12107|FBan0003600 last_updated:000321 *F >EG:BACH61I5.1 *F >Diff : 0 *F >CG16902|FBan0016902|CT37504|FBan0016902 last_updated:000321 *F >EG:133E12.2 *F \#>Diff : B B- B+ C++ *F >Diff : D *F \# *F \#Comments: *F \# *F \#Gene EG:133E12.2 is a 'consensus' prediction of both Genfinder and Genscan *F \#programs. *F \# B : So, differences on internal exons should be due to the different *F \# prediction algorithms used. *F \# C : I had decided to not include the last exons (that both Genfinder and *F \# Genscan predict), because it was indicated by the protein similarity *F \# hits (e.g. O161228, THR4 gene). *F \# *F \#CONCLUSION: EG:133E12.2 is more correct. *F \# *F >CG4406|FBan0004406|CT14360|FBan0004406 last_updated:000321 *F >EG:133E12.3 *F >Diff : A+ *F >CG4399|FBan0004399|CT14236|FBan0004399 last_updated:000321 *F >EG:133E12.4 *F >Diff : 0 *F >CG4376|FBan0004376|CT14163|FBan0004376 last_updated:000321 *F >EG:133E12.1|FBgn0000667;Actn *F >Diff : 0 *F >CG4380|FBan0004380|CT14272|FBan0004380 last_updated:000321 *F >EG:22E5.1|FBgn0003964;usp *F >Diff : 0 *F >CG4325|FBan0004325|CT14153|FBan0004325 last_updated:000321 *F >EG:22E5.12 *F >Diff : 0 *F >CG4322|FBan0004322|CT14137|FBan0004322 last_updated:000321 *F >EG:22E5.11 *F >Diff : C+ *F >CG4313|FBan0004313|CT14076|FBan0004313 last_updated:000321 *F >EG:22E5.10 *F >Diff : 0 *F >CG4290|FBan0004290|CT14053|FBan0004290 last_updated:000321 *F >EG:22E5.8 *F >Diff : 0 *F >CG4281|FBan0004281|CT14023|FBan0004281 last_updated:000321 *F >EG:22E5.7 *F \#>Diff : A- B- C+ *F >Diff : D *F \# *F \#Comments: *F \# *F \#EG:22E5.7 is mainly a Genefinder prediction apart from the first exon. *F \#Both Genefinder and Genscan aggree on the prediction of the first 3-4 *F \#exons, but Genescan extends the gene further down (in the area of *F \#EG:22E5.8). *F \# *F \# A : I had decided to 'truncate' the first exon (appeared on both *F \# predictions), because this was suggested by EST hits *F \# (e.g. AI133907). Thus, i adopted the first ATG found in this EST, as *F \# the starting ATG for gene EG:22E5.7. *F \# CG4281 probably follows the Genscan prediction. *F \# B : I cannot explain gthe differences in the internal exon, since both *F \# Genefinder and Genscan predictions aggree on them. *F \# C : If Celera followed the Genscan (or Genie) prediction 'blindly', then *F \# the \-COOH of CG4281 should match part of EG:22E5.8. *F \# I believe EG:22E5.8 is a different gene, because of EST hits *F \# (e.g. AI108139) and protein similarity hits of different type. *F \# *F \#CONCLUSION: EG:22E5.7 is more correct. *F \# *F >CG4199|FBan0004199|CT13610|FBan0004199 last_updated:000321 *F >EG:22E5.5 *F >Diff : A+ *F >CG4194|FBan0004194|CT13604|FBan0004194 last_updated:000321 *F >EG:22E5.6 *F >Diff : 0 *F >CG4061|FBan0004061|CT13484|FBan0004061 last_updated:000321 *F >EG:22E5.3 *F >Diff : 0 *F >CG4045|FBan0004045|CT13392|FBan0004045 last_updated:000321 *F >EG:22E5.4 *F >Diff : C+ *F >CG4025|FBan0004025|CT13374|FBan0004025 last_updated:000321 *F >EG:22E5.9 *F >Diff : 0 *F >CG3835|FBan0003835|CT42116|FBan0003835 last_updated:000321 *F >EG:87B1.3 *F >Diff : 0 *F >CG3724|FBan0003724|CT12475|FBan0003724 last_updated:000321 *F >EG:87B1.4|FBgn0004654;Pgd *F >Diff : 0 *F >CG3717|FBan0003717|CT12461|FBan0003717 last_updated:000321 *F >EG:87B1.6|FBgn0013432;bcn92 *F >Diff : 0 *F >CG3707|FBan0003707|CT42114|FBan0003707 last_updated:000321 *F >EG:87B1.2|FBgn0004655;wapl *F >Diff : A+ *F \##### WARNING: Known gene \! *F >CG3656|FBan0003656|CT12233|FBan0003656 last_updated:000321 *F >EG:87B1.1|FBgn0005670;Cyp4d1 *F >Diff : 0 *F >CG3630|FBan0003630|CT12189|FBan0003630 last_updated:000321 *F >EG:152A3.3 *F >Diff : 0 *F >CG3621|FBan0003621|CT12175|FBan0003621 last_updated:000321 *F >EG:152A3.7 *F >Diff : 0 *F >CG3466|FBan0003466|CT11675|FBan0003466 last_updated:000321 *F >EG:152A3.4|FBgn0011576;Cyp4d2 *F >Diff : A- *F \##### WARNING: Known gene \!? *F >CG3461|FBan0003461|CT11665|FBan0003461 last_updated:000321 *F >EG:152A3.5|FBgn0003116;pn *F >Diff : 0 *F >CG3460|FBan0003460|CT11655|FBan0003460 last_updated:000321 *F >EG:152A3.1 *F >Diff : 0 *F >CG3457|FBan0003457|CT11649|FBan0003457 last_updated:000321 *F >EG:17E2.1 *F >Diff : B- *F >CG3456|FBan0003456|CT11643|FBan0003456 last_updated:000321 *F >EG:103B4.3 *F >Diff : A- *F >CG18033|FBan0018033|CT40358|FBan0018033 last_updated:000321 *F >EG:103B4.4 *F >Diff : 0 *F >CG3299|FBan0003299|CT11081|FBan0003299 last_updated:000321 *F >EG:103B4.1|FBgn0004397;Vinc *F >Diff : 0 *F >CG3443|FBan0003443|CT40368|FBan0003443 last_updated:000321 *F >EG:30B8.4|FBgn0003048;pcx *F >Diff : B-- *F >CG3228|FBan0003228|CT10831|FBan0003228 last_updated:000321 *F >EG:30B8.2|FBgn0001330;kz *F >Diff : 0 *F >CG3206|FBan0003206|CT10765|FBan0003206 last_updated:000321 *F >EG:30B8.7 *F >Diff : C *F >CG3193|FBan0003193|CT10256|FBan0003193 last_updated:000321 *F >EG:30B8.1|FBgn0000377;crn *F >Diff : 0 *F >CG3191|FBan0003191|CT10198|FBan0003191 last_updated:000321 *F >EG:30B8.3 *F >Diff : 0 *F >CG3078|FBan0003078|CT9973|FBan0003078 last_updated:000321 *F >EG:30B8.6 *F >Diff : D *F \# *F \#Comments: *F \# *F \#EG:30B8.6 is the intact prediction of both Genefinder and Genscan with *F \#good scores. The two first exons supported by EST hits and exon-2 has *F \#also some protein hits (not very strong though). *F \#CG3078 matches EG:30B8.6 only in the first 184 a.a. *F \#I don't know what happened with the rest of the prediction. *F \# *F \#CONCLUSION: Ambigious or EG:30B8.6 is more correct. *F \# *F >CG3071|FBan0003071|CT41361|FBan0003071 last_updated:000321 *F >EG:25E8.3 *F >Diff : B+ *F >CG2924|FBan0002924|CT42120|FBan0002924 last_updated:000321 *F >EG:25E8.2 *F >Diff : A+ C- *F >CG2918|FBan0002918|CT9894|FBan0002918 last_updated:000321 *F >EG:25E8.1 *F >Diff : 0 *F >CG2879|FBan0002879|CT9868|FBan0002879 last_updated:000321 *F >EG:25E8.6 *F >Diff : D *F \##### Note: EG:25E8.6:235-283 matches CG2879:1-49. *F \# *F \#Comments: *F \# *F \#EG:25E8.6 is a small gene predicted by both Genefinder and Genscan with *F \#marginally good scores. It contains small repeats, thus i am not sure *F \#about its true existence. *F \#There are no other supportive evidence for this gene (the protein *F \#similarity hits are not convincing, as it is written in XDrosDB). *F \#The start of CG2879 matches the end of EG:25E8.6. If CG2879 extends *F \#further down, it should match gene EG:25E8.1. *F \# *F \#CONCLUSION: Ambigious. *F \# *F >CG2865|FBan0002865|CT9798|FBan0002865 last_updated:000321 *F >EG:25E8.4 *F >Diff : 0 *F >CG2845|FBan0002845|CT9736|FBan0002845 last_updated:000321 *F >EG:BACH48C10.3|FBgn0003079;phl *F >Diff : B+ *F \##### WARNING: Known gene \! *F >CG7952|FBan0007952|CT23972|FBan0007952 last_updated:000321 *F >EG:BACH7M4.5 *F >Diff : 0 *F >CG7925|FBan0007925|CT23896|FBan0007925 last_updated:000321 *F >EG:BACH59J11.1|FBgn0003714;tko *F >Diff : 0 *F >CG7803|FBan0007803|CT23694|FBan0007803 last_updated:000321 *F >EG:BACH59J11.3|FBgn0004050;z *F >Diff : 0 *F >CG9659|FBan0009659|CT27300|FBan0009659 last_updated:000321 *F >EG:BACR25B3.8|FBgn0001404;egh *F >Diff : 0 *F >CG8590|FBan0008590|CT24639|FBan0008590 last_updated:000321 *F >EG:BACR25B3.9 *F >Diff : 0 *F >CG9900|FBan0009900|CT25082|FBan0009900 last_updated:000321 *F >EG:BACR7C10.3|FBgn0004643;mit(1)15 *F >Diff : 0 *F \##### NOTE: 100% match on the first 260 a.a. only! *F \##### WARNING: Known gene \! *F \# *F \#Comments: *F \#This was a partial version of the gene (splitted between two clones). The *F \#complete version sent to Melanie on 4-Sep-2000. Coparison with CG9900 *F \#showed (almost) 100% a.a. identity over the whole 721 a.a. *F \# *F >CG2621|FBan0002621|CT8875|FBan0002621 last_updated:000321 *F >EG:155E2.3|FBgn0003371;sgg (/partial; this is the 3' terminus) *F >Diff : A+ *F \# *F \#Comments: *F \#This was a partial version of the gene. The complete version sent to *F \#Melanie on 4-Sep-2000. Comparison with CG9900 showed (almost) 100% *F \#a.a. identity over the whole 721 a.a. *F \# *F \#After checking, i found that EG:155E2.3 is practically identical to Q27605 *F \#'PROTEIN KINASE SHAGGY, SGG46 ISOFORM (EC 2.7.1.-) (PROTEIN ZESTE-WHITE 3) *F \#(SGG46).' *F \# *F >CG2655|FBan0002655|CT8939|FBan0002655 last_updated:000321 *F >EG:155E2.2|FBgn0011276;HLH3B *F >Diff : 0 *F >CG2652|FBan0002652|CT8935|FBan0002652 last_updated:000321 *F >EG:155E2.5 *F >Diff : 0 *F >CG2647|FBan0002647|CT8963|FBan0002647 last_updated:000321 *F >EG:155E2.4|FBgn0003068;per *F >Diff : A- B+ *F \##### WARNING: Known gene \! *F \# *F \#Comments: *F \# *F \#There are four alternatively splice per genes reported by EDGP. *F \# *F \#CONCLUSION: No differences really. *F \# *F >CG2650|FBan0002650|CT8975|FBan0002650 last_updated:000321 *F >EG:155E2.1|FBgn0000092;anon-3B1.2 *F >Diff : B- *F >CG2658|FBan0002658|CT8999|FBan0002658 last_updated:000321 *F >EG:100G10.7 *F >Diff : 0 *F >CG2662|FBan0002662|CT9011|FBan0002662 last_updated:000321 *F >EG:100G10.6 *F >Diff : 0 *F >CG2675|FBan0002675|CT9063|FBan0002675 last_updated:000321 *F >EG:100G10.5 *F >Diff : A+ *F >CG2677|FBan0002677|CT9073|FBan0002677 last_updated:000321 *F >EG:100G10.3 *F >Diff : 0 *F >CG2680|FBan0002680|CT9081|FBan0002680 last_updated:000321 *F >EG:100G10.4 *F >Diff : B+ *F >CG2681|FBan0002681|CT9087|FBan0002681 last_updated:000321 *F >EG:100G10.2 *F >Diff : B- *F >CG2685|FBan0002685|CT9103|FBan0002685 last_updated:000321 *F >EG:100G10.1 *F >Diff : 0 *F >CG2694|FBan0002694|CT9121|FBan0002694 last_updated:000321 *F >EG:100G10.8 *F >Diff : 0 *F >CG2701|FBan0002701|CT9167|FBan0002701 last_updated:000321 *F >EG:95B7.9 *F >Diff : 0 *F >CG2706|FBan0002706|CT9201|FBan0002706 last_updated:000321 *F >EG:95B7.8|FBgn0000928;fs(1)Yb *F >Diff : 0 *F >CG2707|FBan0002707|CT9213|FBan0002707 last_updated:000321 *F >EG:95B7.4|FBgn0000927;fs(1)Ya *F >Diff : A- *F >CG2709|FBan0002709|CT9223|FBan0002709 last_updated:000321 *F >EG:95B7.5 *F >Diff : 0 *F >CG2711|FBan0002711|CT9225|FBan0002711 last_updated:000321 *F >EG:95B7.6 *F >Diff : 0 *F >CG2713|FBan0002713|CT9227|FBan0002713 last_updated:000321 *F >EG:95B7.3 *F >Diff : 0 *F >CG2712|FBan0002712|CT9229|FBan0002712 last_updated:000321 *F >EG:95B7.7 *F >Diff : 0 *F >CG2714|FBan0002714|CT9231|FBan0002714 last_updated:000321 *F >EG:95B7.2|FBgn0000376;crm *F >Diff : 0 *F >CG2715|FBan0002715|CT9233|FBan0002715 last_updated:000321 *F >EG:95B7.11 *F >Diff : 0 *F >CG2759|FBan0002759|CT9359|FBan0002759 last_updated:000321 *F >EG:BACN33B1.1|FBgn0003996;w *F >Diff : 0 *F >EG:BACR43E12.6 *F >CG14417|FBan0014417|CT34074|FBan0014417 last_updated:000321 *F >Diff : 0 *F >EG:BACR43E12.5 *F >CG14418|FBan0014418|CT34075|FBan0014418 last_updated:000321 *F >Diff : B+ *F >CG3591|FBan0003591|CT12085|FBan0003591 last_updated:000321 *F >EG:100G7.2|FBgn0014097;anon-3Cb *F >Diff : 0 *F >CG3598|FBan0003598|CT12109|FBan0003598 last_updated:000321 *F >EG:100G7.3 *F >Diff : 0 # *U FBrf0133223 *a Benos *b T. *t 2000.12.13 *T personal communication to FlyBase *u FlyBase error report on Wed Dec 13 15:13:37 2000. *F From benos@ebi.ac.uk Wed Dec 13 15:14:47 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 13 Dec 2000 15:14:47 \+0000 *F Date: Wed, 13 Dec 2000 15:13:37 \+0000 (GMT) *F From: Takis Benos *F To: flybase-updates@morgan.harvard.edu *F cc: Eleanor Whitfield *F Subject: CG_but_not_EG genes.... *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='1053048513-877754332-976720417=:45284' *F Content-Length: 23802 *F Sorry to bother you again.... *F Here is an anlysis i performed on div. 1-3 genes, reported by Celera (in *F the version 1.0 of the Drosophila genome); but with no corresponding EDGP *F prediction. *F Some are small and/or underpredictions. The rest are wrong (i think!). *F Please, let me know if there are any questions, etc... *F Best regards, *F takis ;) *F From benos@stemloop.wustl.edu Wed Dec 13 15:08:39 2000 *F Date: Wed, 13 Dec 2000 09:08:14 \-0600 *F From: Takis Benos *F To: benos@ebi.ac.uk *F \----- Begin Included Message \----- *F >From ma11@gen.cam.ac.uk Wed Oct 11 19:37:22 2000 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 11 Oct 2000 19:37:22 \+0100 *F To: benos@ebi.ac.uk *F Subject: CG but not EDGP *F Cc: ma11@gen.cam.ac.uk, m.gatt@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Wed, 11 Oct 2000 19:37:25 \+0100 *F Content-Length: 3583 *F Takis *F Mellanie and I have just realised that the CG genes not predicted *F by EDGP are non-random. There are runs of sequential or nearly *F sequential CG numbers, which means that the genes are very close. *F This strikes us as peculiar and odd and suspicious. Have you any ideas ? *F Some are indeed very small and must be wrong, but by NO means all \! *F M & M *F CG genes not obviously predicted by EDGP: *F (genes between '===' lines are tandemly arranged in the genome) *F ============================================================================== *F == *F CG13376 X CT32708 1 213 (-) GeneScene *F Start: cnt_1:25974 (frame=-2) *F PVB: CG13376 small (60-70 a.a.) with low Genefinder score (sc=11.28). *F Similarly low score for the Genscan prediction. *F ============================================================================== *F == *F CG13373 X CT32703 3 1038 (+) *F GeneScene *F Start: cnt_1:230772 (frame=+3) *F PVB: CG13373 is in a partially triplicated region. *F WARNING: possible mis-assembly in Celera's sequence; in JG, gene CG13373 *F was supposed to be close to CG13376 (see above)! *F CG18275 X CT41450 1 187 (-) GeneScene *F Start: cnt_1:231936 (frame=-1) *F PVB: CG18275 is in the same partially triplicated region. *F CG3176 X receptor CT10645 1 65 (+) GeneScene *F Start: cnt_1:235283 (frame=+2) *F PVB: CG3176 is in the same partially triplicated region. *F CG18273 X CT41446 3 1449 (-) *F GeneScene *F PVB: CG18273 is gene EG:171D11.6. *F CG18166 X CT40990 2 638 (-) GeneScene *F PVB: CG18166 is partial duplication of gene EG:171D11.6 (see above). *F ============================================================================== *F == *F CG13365 X CT32692 1 494 (-) GeneScene *F Start: cnt_1:365582 (frame=-2) *F PVB: CG13365 is in a partially duplicated region (as it shown from *F partially duplicated EST:AI294113). *F ============================================================================== *F == *F CG13362 X CT32687 1 768 (-) GeneScene *F PVB: No comment. I couldn't locate it. Too many partial hits. *F The 'gene' is obviously from repetitive region. *F >CG13362 *F MLPGAQYDPYGMTSYAAGRRHDSVSRQEVTAPTDLSNAPSSSRTTSTTP *F APTTTTTTTTTTTPAPTTRPSTTTTSTTPPPVPPPPQSTSSSFSEGPTS *F SLLRFGEYPAYNRRVNNLYNARPQYPYPDYFNYQPQQQTVVSEQGVSSN *F SRIQFVPCMCPVSMPSFVSSSTAATLPSQLSTSSTSFVSQPAARHIEGQ *F ELEAEVDGETDNEGEEEDEDEEQGQGQEQDQGQSQSHSLEGIAIKAQTE *F RQDITSDSPV *F CG13361 X CT32686 2 1335 (-) *F GeneScene *F Start: cnt_1:497318 (frame=-2) *F PVB: CG13361 probably corresponds to predictions BACR19J1.e (Genefinder; *F score=34.92) and/or BACR19J1.gs.3 (Genscan). EST:AA140953 which *F covers one of the predicted exons, extends into a region with stop *F codons in all three frames. This made me believe that the EST should *F be in a UTR region; thus there should be no (coding) gene CG13361. *F ============================================================================== *F == *F CG14635 X CT34396 1 360 (+) GeneScene *F Start: cnt_1:583083 (frame=+3) *F PVB: CG14635 is small and i have no prediction in this area (not in this *F strand anyway). *F ============================================================================== *F == *F CG14633 X CT34393 1 831 (-) GeneScene *F Start: cnt_1:619985 (frame=-2) *F PVB: CG14633 corresponds to prediction BACR7A4.q (Genefinder; *F score=33.27). It was not reported due to lack of additional evidence. *F CG11663 X CT34392 2 573 (+) GeneScene *F Start: cnt_1:632851 (frame=+1) *F PVB: The only prediction in the area is BACR7A4.u (Genefinder; sc=27.99). *F The prediction was not reported due to low score and lack of any *F other supportive evidence(s). Moreover, the conceptually translated *F peptide is of very very low complexity. *F >CG11663 *F MANPKSSGGNKSKGKGHQHRQSQQNSHQQQQQQQQQQSQQSQQPQMQTQI *F TPAPVASTNLNTPTATPLASHPSEDTLALAAAVAASIPAAPLARPLPDRR *F TTTPAVVTTTSNSSSETRNASENLATSRTASAAVAASENRRGILQRLFGW *F SS *F CG14632 X CT34391 4 1676 (-) *F GeneScene *F Start: cnt_1:632537 (part of it; frame=-2) *F PVB: No predictions in this area; but the gene is of low complexity. *F >CG14632 *F MSDEVPLGRLSHIFDTLTNLQQQQHLRSQEQLHSQQHPHSQLQPEPQQS *F SAEIRRRSASSSPSPSASASASTSGRATPSLGEVAGSGYLHTFPSHFYH *F HQVHHLQQHSQPPSLPTQLGAARGSQSLQGSPLLAKRATSFSGQIPLAQ *F GRFTASGTTAASGAIGLPASTPNSPRLLPRRAPRPPPIPAKPNQVKADQ *F QSKDAQARNSTTTTVQATVNPVLAALDAPDAPWPHFSTLTEHLDVHQVN *F NYGQALPQINWQERCLELQLELHRSKNQAGRIRDMLREKETLFS *F ============================================================================== *F == *F CG11639 X transcription CT34386 2 405 (-) GeneScene *F Start: cnt_1:749792 (frame=-2) *F PVB: CG11639 is gene EG:BACR7A4.7. *F CG14631 X CT34389 1 399 (+) GeneScene *F Start: cnt_1:750360 (frame=+3) *F PVB: CG14631 corresponds to prediction BACR7A4.ag (Genefinder sc=27.98). *F It was not reported due to low score and lack of other supportive *F evidence. *F ============================================================================== *F == *F CG11393 X CT31813 1 353 (-) GeneScene *F Start: cnt_1:881702 (frame=-2) *F PVB: CG11393 is a very small gene (52 a.a.). By the coordinates of the *F hit, it must be included in EG:BACR42I17.1 region. *F I am very confident about EG:BACR42I17.1. It is supported by protein *F and EST hits and it contains motif PS00813 (IF4E). I don't know *F what's wrong with Celera's prediction. *F ============================================================================== *F == *F CG11381 X transcription CT31772 1 1365 (+) *F GeneScene *F PVB: CG11381 is (part of) gene BACR42I17.9 (reported). *F ============================================================================== *F == *F CG14770 X CT34578 2 694 (+) GeneScene *F Start: cnt_1:1086807 (frame=+3) *F PVB: CG14770 corresponds to Genscan prediction 132E8.gs.2. The Genscan *F score is low; there is no Genefinder prediction in this region and *F no other supportive evidence. Thus, it was not reported. *F ============================================================================== *F == *F CG14771 X transcription CT34579 3 2318 (+) *F GeneScene *F CG14772 X CT34580 2 673 (+) GeneScene *F PVB: There are two huge predictions in this region *F (cnt_1:1095100-1120000). One from Genefinder and one from Genescan. *F Very untypical for Drosophila genes (many small exons, big introns). *F The two predictions do not agree in many of the exons; and none *F includes region indicated from an EST cluster (e.g. EST:AI062494). *F The general genome organisation/evidences, made me suspicious and *F thus i reported only the exons i was confident about, based on *F protein similarity hits (gene EG:132E8.4). *F ============================================================================== *F == *F CG14778 X CT34588 4 743 (+) GeneScene *F Start: cnt_1:1187350 (frame=+1) *F PVB: CG14778 corresponds to Genefinder prediction 80H7.i. It was not *F reported due to low score (sc=34.6) and lack of further supportive *F evidence(s). *F ============================================================================== *F == *F CG3080 X CT10352 3 2540 (+) *F GeneScene *F Start: cnt_1:1314702 (frame=+3) *F PVB: I am not sure what CG3080 corresponds to. It looks like it's the *F 'tail' of a Genscan prediction. But there is no supportive evidence *F in the whole region. *F CG3729 X CT12497 2 845 (-) GeneScene *F PVB: Small (101 a.a.) and repetitive. I cannot locate it. Should be *F Genscan prediction 25D2.gs.1; which has very low score and no *F suportive evidence. *F >CG3729 *F MLCYVSLTIRRLHSLAPHCQLDAALDAVHWPLAPGPCPPSAIWHPPSPL *F IQMLCRSAAIKITRQTTAAEQLKQKKKKKKEKEKRSGKRQQRKRKSGRG *F G *F ============================================================================== *F == *F CG14797 X CT34609 3 787 (+) GeneScene *F Start: cnt_1:1397298 (frame=+3) *F PVB: CG14797 probably corresponds to (non-reported) prediction 9D2.gs.1 *F (Genscan). This prediction as well as a similar (in all but one *F exons) from Genefinder (pred. gene 9D2.j; sc=27.95) were not reported *F due to their low score and lack of any other supportive evidences. *F ============================================================================== *F == *F CG14798 X CT34610 2 376 (+) GeneScene *F Start: cnt_1:1406487 (frame=+3) *F PVB: CG14798 probably corresponds to the (non-reported) predicted gene *F 9D2.n (Genefinder; sc=23.26). The prediction was not reported due to *F the low score, the absence of other supporting evidence(s) and the *F overlap of its third exon with (reported) gene EG:9D2.3 (on the *F reverse strand). *F ============================================================================== *F == *F CG14810 X CT34623 1 556 (-) GeneScene *F Start: cnt_1:1528333 (frame=-3) *F PVB: CG14810 probably corresponds to one of the (non-reported) predictions *F 30B7.gs.6 (Genscan) or 30B7.b (Genefinder; sc=19.37). The gene is *F small, with low score and no other supporting evidence. Moreover it *F is located in a region with a number of small tandem and inverted *F repeats (and remnants of transp. elements). *F CG14811 X CT34624 1 651 (-) GeneScene *F Start: cnt_1:1530386 (frame=-2) *F PVB: CG14811 probably corresponds to (non-reported) predictions 30B7.a *F (Genefinder; sc=26.47) and 30B7.gs.7 (Genscan). The prediction was *F not reported due to the low score and the absence of other supporting *F evidence(s). *F CG14799 X CT34612 2 1018 (+) *F GeneScene *F Start: cnt_1:1533897 (frame=+3) *F PVB: CG14799 corresponds to (non-reported) predictions 30B7.h (Genefinder; *F sc=8.34) and 30B7.gs.8 (Genscan). The prediction was not reported *F due to the low score and the absence of other supporting evidence(s). *F ============================================================================== *F == *F CG14800 X CT34613 3 1260 (+) *F GeneScene *F Start: cnt_1:1551750 (frame=+3) *F PVB: The closest prediction to CG14800 is 131F2.gs.1 (Genscan). Only the *F last exon(s) was reported from this prediction (EG:131F2.2), based on *F protein similarity hits (e.g. SW:BCT5_BOVIN). *F ============================================================================== *F == *F CG14806 X CT34619 3 663 (+) GeneScene *F Start: cnt_1:1588425 (frame=+3) *F PVB: The only predictions i can find in this area are 63B12.e (Genefinder; *F sc=15.36) and 63B12.gs.10. The predictions were not reported due to *F the low score and the absence of other supporting evidence(s). *F ============================================================================== *F == *F CG14819 X CT34632 5 2022 (-) *F GeneScene *F Start: cnt_1:1610637 (frame=-1) *F PVB: CG14819 is probably is 'merged' prediction of 86E4.s (Genefinder; *F sc=18.67) and 86E4.s (Genefinder; sc=18.94). The predictions were *F not reported due to the low score and the absence of other supporting *F evidence(s). Moreover, EST:AA695846 does not agree with prediction *F 86E4.s. *F ============================================================================== *F == *F CG18082 X CT40582 1 222 (+) GeneScene *F Start: cnt_1:1919235 (frame=+3) *F PVB: CG18082 is small (~70 a.a.), with low score and no supporting *F evidences (pred. gene 30B8.a; Genefinder sc=10.05). *F CG14052 X CT33613 3 1785 (+) *F GeneScene *F Start: cnt_1:1919541 (frame=+3) *F PVB: CG14052 corresponds to prediction 30B8.b (Genefinder; sc=24.88). The *F prediction was not reported due to the low score and the absence of *F other supporting evidence(s). Also, the predicted gene is of low *F complexity. *F CG18850 *F Start: cnt_1:1921912 (frame=-3) *F PVB: CG18850 corresponds to prediction 30B8.t (Genefinder; sc=18.05). The *F prediction was not reported due to the low score and the absence of *F other supporting evidence(s). Also, the predicted gene is of low *F complexity. *F WARNING! *F Most importantly, in EDGP sequence THE PREDICTION OVERLAPS WITH GENE *F EG:30B8.4 (also known as pecanex; pcx; FBgn0003048)! However, in *F the JG this gene is predicted between the previous two. *F ============================================================================== *F == *F CG3091 X binding or CT9997 6 1492 (-) *F GeneScene *F Start: cnt_1:1949751 (frame=-1) *F PVB: CG3091 is (partly) predicted gene 30B8.l (Genefinder; sc=34.63). *F This prediction was supported by EST(s), but it was not reported *F because it is consisted of a partial duplication of gene EG:30B8.3 *F (without the protein similarity thoug; different translation frame). *F ============================================================================== *F == *F CG3073 X enzyme CT9957 3 1834 (-) *F GeneScene *F Start: cnt_1:1957696 (frame=-3) *F PVB: CG3073 is probably consisted of the joint Genscan predictions *F 25E8.gs.1 and 30B8.gs.8. This gene is supported by EST hits, *F therefore I SHOULD HAD REPORTED IT. The reason i missed it, is that *F it is located in between two cosmids. *F ============================================================================== *F == *F CG14049 X CT33608 2 402 (-) GeneScene *F Start: cnt_1:2019302 (frame=-2) *F PVB: CG14049 corresponds to prediction BACH48C10.gs.3 and (partly) to *F prediction BACH48C10.e (Genefinder; sc=11.99). The predictions were *F not reported due to the low score and the absence of other supporting *F evidence(s). *F ============================================================================== *F == *F CG7894 X cell adhesion CT23737 3 6037 (-) *F GeneScene *F Start: cnt_1:2129993 (frame=-2) *F PVB: CG7894 is probably consisted of the joint Genefinder predictions *F BACR25B3.n (sc=37.86) and BACH59J11.gs.6 (sc=9.99). This gene is *F supported by EST hits and (weak) protein similarity hits, therefore I *F SHOULD HAD REPORTED IT. The reason i missed it, is that it is *F located in between two BACs. *F ============================================================================== *F == *F CG8310 X transporter CT24372 3 821 (-) GeneScene *F Start: cnt_1:2257964 (frame=-2) *F PVB: CG8310 is gene EG:BACR25B3.4. *F CG8636 X translation CT25021 2 959 (-) GeneScen *F Start: cnt_1:2288033 (frame=-2) *F PVB: CG8636 corresponds to prediction BACR7C10.n (Genefinder; sc=61.87). *F It is supported by (weak) protein hits (corresponding to translation *F factors) as well as ESTs. However, one of the EST hits (AA820554) is *F in close proximity (100bp) from the end of the transposable element *F Burdock (EG:BACR7C10.5) that was also found there. Both the nature *F of the protein hits and the close proximity with the transposable *F element, made me suspicious that this may not be a 'real' Drosophila *F gene. Thus, i did not report it. *F ============================================================================== *F == # *U FBrf0133224 *a Millburn *b G. *t 2000.11.22 *T personal communication to FlyBase *u FlyBase error report on Wed Nov 22 14:23:55 2000. *F From bdgp-admin@fruitfly.bdgp.berkeley.edu Wed Nov 22 02:09:29 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 22 Nov 2000 02:09:29 \+0000 *F To: bdgp@fruitfly.org *F Content-Type: X-sun-attachment *F From: Gillian Millburn (Genetics) *F Date: Tue, 21 Nov 2000 14:23:55 \+0000 *F Subject: BDGP EST GH02584 *F Sender: bdgp-admin@fruitfly.bdgp.berkeley.edu *F X-BeenThere: bdgp@fruitfly.BDGP.Berkeley.EDU *F X-Mailman-Version: 2.0beta6 *F List-Help: *F List-Post: *F List-Subscribe: , *F *F List-Id: BDGP mailing list *F List-Unsubscribe: , *F *F Content-Length: 41567 *F hello peeps, *F here's a complicated one (sorry!). *F I was trying to sort out a couple of merges between CGs and EG genes *F and came across this gnarly thing. *F The problem is in the region which the EST GH02584.5prime maps to (this *F is in 1B). *F Here is a picture of the relevant bit of GeneSeen: *F ................................................................................ *F | *F CG3176 | EST GH02584.3prime *F \-> v <--- *F \---------------------------------------//--///------------------------------- *F <-- <--------- <---- <------------- *F CG18275 CG18273 CG18166 CG13372 *F (annotated as EG:171D11.6) *F <-- *F EST GH02584.5prime *F ................................................................................ *F 1. If you look on GeneSeen, EST GH02584.5prime maps in between annotations. *F It is between CG18275 and CG18273 on the bottom strand. *F 2. However, if you look at the GadFly page for CG18166, homology to EST *F GH02584.5prime is given as evidence for this annotation. *F (http://fly.ebi.ac.uk:7081/cgi-bin/annot/gene?CG18166) *F (apologies for English URL \!) *F 3. However, if you look at the EST page for GH02584.5prime, CG3176 is given as *F the gene it corresponds to *F (http://www.fruitfly.org/cgi-bin/EST/community_query/cloneReport.pl?id_type=0&i *F d_value=GH02584&db_name=estlabtrack) *F Clone ID: GH02584 *F Gene: CG3176 (CG3176) *F 4. I figured I'd use the GH02584.5prime and GH02584.3prime sequences to *F BLAST against the genome to see what is going on, and it turns out that *F this region is repetitive (which might explain the gaps in the sequence *F I guess). This is scaffold AE003417.1 *F File of BLAST results for GH02584.5prime and GH02584.3prime (called *F 'BLAST' is attached). *F I think that the EST sequences are repeated at least 3-4 times in the *F region (both the 3' and 5' sequences are repeated) that spans *F approximately from coordinates 233851-250940 of AE003417.1 (this *F accession has an October date and is therefore release 2, so the *F sequence cannot have changed from release 1 as it has a .1 in the *F accession number). The repetitive region includes the following CGs: *F CG18275, CG3176, CG18273, CG18166, CG13372. *F This explains probably why CG18166 has GH02584.5prime as evidence and *F yet GH02584.5prime appears to map to the left of CG18166 on GeneSeen. *F It also probably explains why GH02584.3prime appears to be upstream of *F GH02584.5prime on GeneSeen :), GeneSeen is probably just showing a *F different repeat for the 5' and 3' EST sequences. *F I will curate this as an error report, as I guess care should be taken *F in annotating this repetitive region and in using EST GH02584 as *F evidence and I guess its possible the assembly is not correct. *F I have sent this to bdgp, because I think the EST page for GH02584 *F needs changing \- I don't think it should have the gene as 'CG3176' *F regardless of whether or not the region is repeated, because CG3176 is *F on the opposite strand from the EST. I thought that the direction of *F the match was being taken into account now when matching up ESTs and *F CGs ? *F Also, how are ESTs being filtered for display on GeneSeen \- if they *F match to more than one region, are they still being shown, as seems to *F be the case for this EST or are they not being displayed on GeneSeen *F (rather like the P-elements, where multiple inserts are not being *F displayed). Perhaps this one got through a filter because the repeat *F is WITHIN a scaffold rather than the EST hitting more than one scaffold *F ? *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F \------------------------------------------------------------------------------- *F RID: 974811863-24859-18732 *F Query= GH02584.5prime *F (613 letters) *F Database: D. melanogaster genomic nucleotide sequences *F 1170 sequences; 122,655,632 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003417.1|AE003417 Drosophila melanogaster genomic scaf... 650 0.0 *F Alignments *F >gb|AE003417.1|AE003417 Drosophila melanogaster genomic scaffold *F 142000013386054 section 1 of 35, *F complete sequence *F Length = 314661 *F Score = 650 bits (328), Expect = 0.0 *F Identities = 331/332 (99%) *F Strand = Plus / Minus *F Query: 194 aggaccctcttcatctttcgtggtccgcgggggtcgcccatttctgttcaagattgtctg 253 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237566 aggaccctcttcatctttcgtggtccgcgggggtcgcccatttctgttcaagattgtctg *F 237507 *F Query: 254 cccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagcttcta 313 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237506 cccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagcttcta *F 237447 *F Query: 314 ttcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaaggat 373 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237446 ttcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaaggat *F 237387 *F Query: 374 aacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtgatc 433 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237386 aacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtgatc *F 237327 *F Query: 434 aagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagagaac 493 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237326 aagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagagaac *F 237267 *F Query: 494 aattcgaaaactgttcgtcctcttggcactcg 525 *F ||||||||||||||||||||||||| |||||| *F Sbjct: 237266 aattcgaaaactgttcgtcctcttgtcactcg 237235 *F Score = 611 bits (308), Expect = e-174 *F Identities = 327/332 (98%), Gaps = 1/332 (0%) *F Strand = Plus / Minus *F Query: 194 aggaccctcttcatctttcgtggtccgcgggggtcgcccatttctgttcaagattgtctg 253 *F ||||||||||||||||||||||||| |||||||||| ||||||||||||||||||||||| *F Sbjct: 246212 aggaccctcttcatctttcgtggtctgcgggggtcggccatttctgttcaagattgtctg *F 246153 *F Query: 254 cccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagcttcta 313 *F |||||||| |||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 246152 cccatggt-ctcagctctgagctactccaggatgccctgcggtccagcaacaagcttcta *F 246094 *F Query: 314 ttcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaaggat 373 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 246093 ttcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaaggat *F 246034 *F Query: 374 aacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtgatc 433 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 246033 aacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtgatc *F 245974 *F Query: 434 aagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagagaac 493 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 245973 aagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagagaac *F 245914 *F Query: 494 aattcgaaaactgttcgtcctcttggcactcg 525 *F ||||||||||||||||||||||||| |||||| *F Sbjct: 245913 aattcgaaaactgttcgtcctcttgtcactcg 245882 *F Score = 593 bits (299), Expect = e-168 *F Identities = 305/307 (99%) *F Strand = Plus / Minus *F Query: 194 aggaccctcttcatctttcgtggtccgcgggggtcgcccatttctgttcaagattgtctg 253 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240648 aggaccctcttcatctttcgtggtccgcgggggtcgcccatttctgttcaagattgtctg *F 240589 *F Query: 254 cccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagcttcta 313 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240588 cccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagcttcta *F 240529 *F Query: 314 ttcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaaggat 373 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240528 ttcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaaggat *F 240469 *F Query: 374 aacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtgatc 433 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240468 aacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtgatc *F 240409 *F Query: 434 aagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagagaac 493 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 240408 aagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagaaaac *F 240349 *F Query: 494 aattcga 500 *F | ||||| *F Sbjct: 240348 atttcga 240342 *F Score = 420 bits (212), Expect = e-116 *F Identities = 254/268 (94%) *F Strand = Plus / Minus *F Query: 251 ctgcccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagctt 310 *F ||||||||||||||||| ||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 251207 ctgcccatggttctcagttctgagctgctccaggatgccttgcggtccagcaacaagctt *F 251148 *F Query: 311 ctattcgaatgctatggcagcctgatcagatgctatcaggagtgcgttgaacttcgcaag 370 *F |||||||||||||||||||||||| |||||||| |||| ||||||| |||||||||||| *F Sbjct: 251147 ctattcgaatgctatggcagcctgttcagatgccatcaaaagtgcgtcgaacttcgcaag *F 251088 *F Query: 371 gataacaaggatcggactaagagccaagactactgggatgcagagtttgtaggttctgtg 430 *F |||||||||||||||||| ||||||||||| ||||||||||||||||||||| | ||||| *F Sbjct: 251087 gataacaaggatcggactgagagccaagaccactgggatgcagagtttgtagttcctgtg *F 251028 *F Query: 431 atcaagcagctggcagagatggttcgccgctctcaggagcctgaaaagctgttagaagag 490 *F |||||||||||| ||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 251027 atcaagcagctgacagagatggttcgccgctctcaggagcctgaaaagcttttagaagag *F 250968 *F Query: 491 aacaattcgaaaactgttcgtcctcttg 518 *F ||||||||||||||||||||||||||| *F Sbjct: 250967 tacaattcgaaaactgttcgtcctcttg 250940 *F Score = 389 bits (196), Expect = e-107 *F Identities = 196/196 (100%) *F Strand = Plus / Minus *F Query: 1 tttattgccaatctcttcgtggggttatccccgtaaatttgtggtcgctgctttgctcca 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237798 tttattgccaatctcttcgtggggttatccccgtaaatttgtggtcgctgctttgctcca *F 237739 *F Query: 61 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237738 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa *F 237679 *F Query: 121 gcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctccattac 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 237678 gcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctccattac *F 237619 *F Query: 181 aggaagtaggagtagg 196 *F |||||||||||||||| *F Sbjct: 237618 aggaagtaggagtagg 237603 *F Score = 389 bits (196), Expect = e-107 *F Identities = 196/196 (100%) *F Strand = Plus / Minus *F Query: 1 tttattgccaatctcttcgtggggttatccccgtaaatttgtggtcgctgctttgctcca 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240880 tttattgccaatctcttcgtggggttatccccgtaaatttgtggtcgctgctttgctcca *F 240821 *F Query: 61 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240820 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa *F 240761 *F Query: 121 gcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctccattac 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 240760 gcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctccattac *F 240701 *F Query: 181 aggaagtaggagtagg 196 *F |||||||||||||||| *F Sbjct: 240700 aggaagtaggagtagg 240685 *F Score = 337 bits (170), Expect = 2e-91 *F Identities = 182/186 (97%) *F Strand = Plus / Minus *F Query: 1 tttattgccaatctcttcgtggggttatccccgtaaatttgtggtcgctgctttgctcca 60 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 246443 tttattgccaatctcttcgtggggttatcctcgtaaatttgtggtcgctgctttgctcca *F 246384 *F Query: 61 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 246383 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa *F 246324 *F Query: 121 gcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctccattac 180 *F |||||||||||||||||||||||||||||| ||||||||||| |||||||||||||||| *F Sbjct: 246323 gcccgacataactgcactctgagcggcgctgtctctccgatctcccacccgctccattac *F 246264 *F Query: 181 aggaag 186 *F |||||| *F Sbjct: 246263 aggaag 246258 *F Score = 337 bits (170), Expect = 2e-91 *F Identities = 182/186 (97%) *F Strand = Plus / Minus *F Query: 1 tttattgccaatctcttcgtggggttatccccgtaaatttgtggtcgctgctttgctcca 60 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 235311 tttattgccaatctcttcgtggggttatcctcgtaaatttgtggtcgctgctttgctcca *F 235252 *F Query: 61 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 235251 ttttttgttctcgtgtttaacggtttaaactgaactcatctttgcattgagcaacaacaa *F 235192 *F Query: 121 gcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctccattac 180 *F |||||||||||||||||||||||||||||| ||||||||||| |||||||||||||||| *F Sbjct: 235191 gcccgacataactgcactctgagcggcgctgtctctccgatctcccacccgctccattac *F 235132 *F Query: 181 aggaag 186 *F |||||| *F Sbjct: 235131 aggaag 235126 *F Score = 234 bits (118), Expect = 2e-60 *F Identities = 131/134 (97%), Gaps = 1/134 (0%) *F Strand = Plus / Minus *F Query: 392 agccaagactactgggatgcagagtttgtaggttctgtgatcaagcagctggcagagatg 451 *F ||||||||||||||| || ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 242941 agccaagactactggaat-cagagtttgtaggttctgtgatcaagcagctggcagagatg *F 242883 *F Query: 452 gttcgccgctctcaggagcctgaaaagctgttagaagagaacaattcgaaaactgttcgt 511 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 242882 gttcgccgctctcaggagcctgaaaagctgttagaagagaacaattcgaaaactgttcgt *F 242823 *F Query: 512 cctcttggcactcg 525 *F ||||||| |||||| *F Sbjct: 242822 cctcttgtcactcg 242809 *F Score = 210 bits (106), Expect = 3e-53 *F Identities = 125/130 (96%), Gaps = 1/130 (0%) *F Strand = Plus / Minus *F Query: 194 aggaccctcttcatctttcgtggtccgcgggggtcgcccatttctgttcaagattgtctg 253 *F ||||||||||||||||||||||||| |||||||||| ||||||||||||||||||||||| *F Sbjct: 235080 aggaccctcttcatctttcgtggtctgcgggggtcggccatttctgttcaagattgtctg *F 235021 *F Query: 254 cccatggttctcagctctgagctgctccaggatgccctgcggtccagcaacaagcttcta 313 *F ||||||| ||||||||||||||| ||||||||||||||||||||||||| |||||||||| *F Sbjct: 235020 cccatgg-tctcagctctgagctactccaggatgccctgcggtccagcaccaagcttcta *F 234962 *F Query: 314 ttcgaatgct 323 *F |||||||||| *F Sbjct: 234961 ttcgaatgct 234952 *F Score = 153 bits (77), Expect = 6e-36 *F Identities = 86/89 (96%) *F Strand = Plus / Minus *F Query: 525 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggggcttacccatcgtta 584 *F |||||||||||||||||||||||||||||||||||||||||||| ||| ||||||||| | *F Sbjct: 237172 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggcgctcacccatcgtca *F 237113 *F Query: 585 acgtttgcatttggatccggatgagatag 613 *F ||||||||||||||||||||||||||||| *F Sbjct: 237112 acgtttgcatttggatccggatgagatag 237084 *F Score = 153 bits (77), Expect = 6e-36 *F Identities = 86/89 (96%) *F Strand = Plus / Minus *F Query: 525 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggggcttacccatcgtta 584 *F |||||||||||||||||||||||||||||||||||||||||||| ||| ||||||||| | *F Sbjct: 248347 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggcgctcacccatcgtca *F 248288 *F Query: 585 acgtttgcatttggatccggatgagatag 613 *F ||||||||||||||||||||||||||||| *F Sbjct: 248287 acgtttgcatttggatccggatgagatag 248259 *F Score = 153 bits (77), Expect = 6e-36 *F Identities = 86/89 (96%) *F Strand = Plus / Minus *F Query: 525 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggggcttacccatcgtta 584 *F |||||||||||||||||||||||||||||||||||||||||||| ||| ||||||||| | *F Sbjct: 240249 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggcgctcacccatcgtca *F 240190 *F Query: 585 acgtttgcatttggatccggatgagatag 613 *F ||||||||||||||||||||||||||||| *F Sbjct: 240189 acgtttgcatttggatccggatgagatag 240161 *F Score = 153 bits (77), Expect = 6e-36 *F Identities = 86/89 (96%) *F Strand = Plus / Minus *F Query: 525 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggggcttacccatcgtta 584 *F |||||||||||||||||||||||||||||||||||||||||||| ||| ||||||||| | *F Sbjct: 245819 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggcgctcacccatcgtca *F 245760 *F Query: 585 acgtttgcatttggatccggatgagatag 613 *F ||||||||||||||||||||||||||||| *F Sbjct: 245759 acgtttgcatttggatccggatgagatag 245731 *F Score = 153 bits (77), Expect = 6e-36 *F Identities = 86/89 (96%) *F Strand = Plus / Minus *F Query: 525 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggggcttacccatcgtta 584 *F |||||||||||||||||||||||||||||||||||||||||||| ||| ||||||||| | *F Sbjct: 242744 gttgaactatcgcgatgctaatgcgacacgcttgctgaatgtggcgctcacccatcgtca *F 242685 *F Query: 585 acgtttgcatttggatccggatgagatag 613 *F ||||||||||||||||||||||||||||| *F Sbjct: 242684 acgtttgcatttggatccggatgagatag 242656 *F Score = 83.8 bits (42), Expect = 4e-15 *F Identities = 57/62 (91%) *F Strand = Plus / Minus *F Query: 453 ttcgccgctctcaggagcctgaaaagctgttagaagagaacaattcgaaaactgttcgtc 512 *F |||| |||||||||| |||||||||||||| ||||||| |||||||| |||||||||||| *F Sbjct: 234908 ttcgacgctctcaggggcctgaaaagctgtaagaagagtacaattcgcaaactgttcgtc *F 234849 *F Query: 513 ct 514 *F || *F Sbjct: 234848 ct 234847 *F Score = 69.9 bits (35), Expect = 7e-11 *F Identities = 56/63 (88%) *F Strand = Plus / Minus *F Query: 32 cgtaaatttgtggtcgctgctttgctccattttttgttctcgtgtttaacggtttaaact 91 *F |||||||| | |||||||||||||||| |||||||||||||| || |||| ||| ||||| *F Sbjct: 233471 cgtaaattagaggtcgctgctttgctcaattttttgttctcgcgtctaactgttcaaact *F 233412 *F Query: 92 gaa 94 *F ||| *F Sbjct: 233411 gaa 233409 *F Score = 61.9 bits (31), Expect = 2e-08 *F Identities = 37/39 (94%) *F Strand = Plus / Minus *F Query: 354 gcgttgaacttcgcaaggataacaaggatcggactaaga 392 *F |||||||| ||||||||||||| |||||||||||||||| *F Sbjct: 234947 gcgttgaatttcgcaaggataataaggatcggactaaga 234909 *F Score = 60.0 bits (30), Expect = 6e-08 *F Identities = 61/70 (87%), Gaps = 1/70 (1%) *F Strand = Plus / Minus *F Query: 115 caacaagcccgacataactgcactctgagcggcgcttcctctccgatctaccacccgctc 174 *F ||||||| ||| |||||||| |||||||||||||| |||||| |||| |||||| ||| *F Sbjct: 233403 caacaagtccgggataactgcgctctgagcggcgctatctctccaatctcccaccc-ctc *F 233345 *F Query: 175 cattacagga 184 *F |||||||||| *F Sbjct: 233344 cattacagga 233335 *F Database: D. melanogaster genomic nucleotide sequences *F Posted date: Nov 13, 2000 4:43 PM *F Number of letters in database: 122,655,632 *F Number of sequences in database: 1170 *F Lambda K H *F 1.37 0.711 1.31 *F Gapped *F Lambda K H *F 1.37 0.711 1.31 *F Matrix: blastn matrix:1 \-3 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 36288 *F Number of Sequences: 1170 *F Number of extensions: 36288 *F Number of successful extensions: 34 *F Number of sequences better than 10.0: 14 *F length of query: 613 *F length of database: 122,655,632 *F effective HSP length: 18 *F effective length of query: 595 *F effective length of database: 122,634,572 *F effective search space: 72967570340 *F effective search space used: 72967570340 *F T: 0 *F A: 0 *F X1: 6 (11.9 bits) *F X2: 10 (19.8 bits) *F S1: 12 (24.3 bits) *F S2: 17 (34.2 bits) *F ================================================================================ *F RID: 974814393-18387-30096 *F Query= GH02584.3prime *F (541 letters) *F Database: D. melanogaster genomic nucleotide sequences *F 1170 sequences; 122,655,632 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003417.1|AE003417 Drosophila melanogaster genomic scaf... 1072 0.0 *F >gb|AE003417.1|AE003417 Drosophila melanogaster genomic scaffold *F 142000013386054 section 1 of 35, *F complete sequence *F Length = 314661 *F Score = 1072 bits (541), Expect = 0.0 *F Identities = 541/541 (100%) *F Strand = Plus / Plus *F Query: 1 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 235866 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt *F 235925 *F Query: 61 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 235926 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg *F 235985 *F Query: 121 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 235986 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg *F 236045 *F Query: 181 aagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 236046 aagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac *F 236105 *F Query: 241 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 236106 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac *F 236165 *F Query: 301 attaagcaaaaatctttgatctattctttggaatgctagaaaacttaaatcatgaccttg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 236166 attaagcaaaaatctttgatctattctttggaatgctagaaaacttaaatcatgaccttg *F 236225 *F Query: 361 agttggccaatggcgtttccttaacgtaaaacacaccttttcgaacagcgtcgtgggcct 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 236226 agttggccaatggcgtttccttaacgtaaaacacaccttttcgaacagcgtcgtgggcct *F 236285 *F Query: 421 ttcggttgctaccatttggcgaatggtgaagataagcaggaagggtgcgacttttcacgg 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 236286 ttcggttgctaccatttggcgaatggtgaagataagcaggaagggtgcgacttttcacgg *F 236345 *F Query: 481 ccagtgcctgcgtggcgcgcactgtggccattacagtggccagtttcagacccaagtccg 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 236346 ccagtgcctgcgtggcgcgcactgtggccattacagtggccagtttcagacccaagtccg *F 236405 *F Query: 541 c 541 *F | *F Sbjct: 236406 c 236406 *F Score = 1072 bits (541), Expect = 0.0 *F Identities = 541/541 (100%) *F Strand = Plus / Plus *F Query: 1 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241436 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt *F 241495 *F Query: 61 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241496 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg *F 241555 *F Query: 121 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241556 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg *F 241615 *F Query: 181 aagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241616 aagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac *F 241675 *F Query: 241 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241676 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac *F 241735 *F Query: 301 attaagcaaaaatctttgatctattctttggaatgctagaaaacttaaatcatgaccttg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241736 attaagcaaaaatctttgatctattctttggaatgctagaaaacttaaatcatgaccttg *F 241795 *F Query: 361 agttggccaatggcgtttccttaacgtaaaacacaccttttcgaacagcgtcgtgggcct 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241796 agttggccaatggcgtttccttaacgtaaaacacaccttttcgaacagcgtcgtgggcct *F 241855 *F Query: 421 ttcggttgctaccatttggcgaatggtgaagataagcaggaagggtgcgacttttcacgg 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241856 ttcggttgctaccatttggcgaatggtgaagataagcaggaagggtgcgacttttcacgg *F 241915 *F Query: 481 ccagtgcctgcgtggcgcgcactgtggccattacagtggccagtttcagacccaagtccg 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241916 ccagtgcctgcgtggcgcgcactgtggccattacagtggccagtttcagacccaagtccg *F 241975 *F Query: 541 c 541 *F | *F Sbjct: 241976 c 241976 *F Score = 1059 bits (534), Expect = 0.0 *F Identities = 541/542 (99%), Gaps = 1/542 (0%) *F Strand = Plus / Plus *F Query: 1 gcgtgatggatctctgcgagg-agtgtggctactcctgcggcgagtgcgccaagtttatt 59 *F ||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||| *F Sbjct: 246999 gcgtgatggatctctgcgagggagtgtggctactcctgcggcgagtgcgccaagtttatt *F 247058 *F Query: 60 tgccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctag 119 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247059 tgccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctag *F 247118 *F Query: 120 ggaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaatt 179 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247119 ggaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaatt *F 247178 *F Query: 180 gaagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaa 239 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247179 gaagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaa *F 247238 *F Query: 240 caatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaa 299 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247239 caatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaa *F 247298 *F Query: 300 cattaagcaaaaatctttgatctattctttggaatgctagaaaacttaaatcatgacctt 359 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247299 cattaagcaaaaatctttgatctattctttggaatgctagaaaacttaaatcatgacctt *F 247358 *F Query: 360 gagttggccaatggcgtttccttaacgtaaaacacaccttttcgaacagcgtcgtgggcc 419 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247359 gagttggccaatggcgtttccttaacgtaaaacacaccttttcgaacagcgtcgtgggcc *F 247418 *F Query: 420 tttcggttgctaccatttggcgaatggtgaagataagcaggaagggtgcgacttttcacg 479 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247419 tttcggttgctaccatttggcgaatggtgaagataagcaggaagggtgcgacttttcacg *F 247478 *F Query: 480 gccagtgcctgcgtggcgcgcactgtggccattacagtggccagtttcagacccaagtcc 539 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247479 gccagtgcctgcgtggcgcgcactgtggccattacagtggccagtttcagacccaagtcc *F 247538 *F Query: 540 gc 541 *F || *F Sbjct: 247539 gc 247540 *F Score = 634 bits (320), Expect = 0.0 *F Identities = 326/328 (99%) *F Strand = Plus / Plus *F Query: 1 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 238354 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt *F 238413 *F Query: 61 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 238414 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg *F 238473 *F Query: 121 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 238474 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg *F 238533 *F Query: 181 aagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac 240 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 238534 aagaagaggaggatccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac *F 238593 *F Query: 241 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 238594 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac *F 238653 *F Query: 301 attaagcaaaaatctttgatctattctt 328 *F |||| ||||||||||||||||||||||| *F Sbjct: 238654 attaggcaaaaatctttgatctattctt 238681 *F Score = 605 bits (305), Expect = e-172 *F Identities = 311/313 (99%) *F Strand = Plus / Plus *F Query: 1 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 243924 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt *F 243983 *F Query: 61 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 243984 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg *F 244043 *F Query: 121 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 244044 gaaacccgttagtttaaaaatcaatcgaccatcatcttatttccagtggtaatcgaattg *F 244103 *F Query: 181 aagaagaggaggctccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac 240 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 244104 aagaagaggaggatccgctgtgcgagcactgccagatgttcctcagctagggcatcaaac *F 244163 *F Query: 241 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 244164 aatcaagcaaatgtcaaatgtattacgttttaaagaatatagagctatatagtatttaac *F 244223 *F Query: 301 attaagcaaaaat 313 *F |||| |||||||| *F Sbjct: 244224 attaggcaaaaat 244236 *F Score = 347 bits (175), Expect = 2e-94 *F Identities = 250/274 (91%), Gaps = 2/274 (0%) *F Strand = Plus / Plus *F Query: 270 ttaaagaatatagagctatatagtatttaacattaagcaaaaatctttgatctattcttt 329 *F |||||||||||||| | ||||| | |||||| || |||||||| ||||||||||||| *F Sbjct: 239143 ttaaagaatatagaaccatatacttcttaacacgaaacaaaaatcgttgatctattcttc *F 239202 *F Query: 330 ggaatgctagaaaacttaaatcatgaccttgagttggccaatggcgtttccttaacgtaa 389 *F |||||||| |||||| ||| |||| | || ||||||||||||||| ||||||||||| *F Sbjct: 239203 ggaatgctgcgaaacttgaattttgacttcgacttggccaatggcgttcccttaacgtaa *F 239262 *F Query: 390 aacacaccttttcgaacagcgtcgtgggcctttcggttgctaccatttggcgaatggtga 449 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239263 aacacaccttttcgaacagcgtcgtgggcctttcggttgctaccatttggcgaatggtga *F 239322 *F Query: 450 agataagcaggaagggtgcga--cttttcacggccagtgcctgcgtggcgcgcactgtgg 507 *F ||||||||||||||||||||| || |||||||||||||||||||||||||||||||||| *F Sbjct: 239323 agataagcaggaagggtgcgactctcttcacggccagtgcctgcgtggcgcgcactgtgg *F 239382 *F Query: 508 ccattacagtggccagtttcagacccaagtccgc 541 *F |||||||||| ||||||||||||||||||||||| *F Sbjct: 239383 ccattacagtagccagtttcagacccaagtccgc 239416 *F Score = 262 bits (132), Expect = 8e-69 *F Identities = 207/230 (90%), Gaps = 4/230 (1%) *F Strand = Plus / Plus *F Query: 1 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgcgccaagtttattt 60 *F ||||||||||||||||||||||||||||||||||||||||||||||| ||| ||| |||| *F Sbjct: 233851 gcgtgatggatctctgcgaggagtgtggctactcctgcggcgagtgctccaggttcattt *F 233910 *F Query: 61 gccgcaactgcgtgactttattgtgagttaagtcccagaaggaagaagcatccccctagg 120 *F |||||| |||||||||||||||||||||||||||| | ||||||||||||||| || *F Sbjct: 233911 gccgcagctgcgtgactttattgtgagttaagtccttaa---aagaagcatcccccttgg *F 233967 *F Query: 121 gaaacccgttagtttaaaaatcaatcgaccatcatctta-tttccagtggtaatcgaatt 179 *F ||| || || ||| ||||||||||||||||||||||| ||||||||||||||||| || *F Sbjct: 233968 gaacaccttttatttgaaaatcaatcgaccatcatcttattttccagtggtaatcgagtt *F 234027 *F Query: 180 gaagaagaggaggctccgctgtgcgagcactgccagatgttcctcagcta 229 *F ||||||||| ||| ||| |||||||||||||||||||||||| ||||||| *F Sbjct: 234028 gaagaagagaaggatcccctgtgcgagcactgccagatgttcttcagcta 234077 *F Score = 44.1 bits (22), Expect = 0.003 *F Identities = 25/26 (96%) *F Strand = Plus / Plus *F Query: 196 cgctgtgcgagcactgccagatgttc 221 *F ||||||||||| |||||||||||||| *F Sbjct: 238688 cgctgtgcgagtactgccagatgttc 238713 *F Score = 34.2 bits (17), Expect = 3.3 *F Identities = 17/17 (100%) *F Strand = Plus / Plus *F Query: 250 aatgtcaaatgtattac 266 *F ||||||||||||||||| *F Sbjct: 234161 aatgtcaaatgtattac 234177 *F Database: D. melanogaster genomic nucleotide sequences *F Posted date: Nov 13, 2000 4:43 PM *F Number of letters in database: 122,655,632 *F Number of sequences in database: 1170 *F Lambda K H *F 1.37 0.711 1.31 *F Gapped *F Lambda K H *F 1.37 0.711 1.31 *F Matrix: blastn matrix:1 \-3 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 39600 *F Number of Sequences: 1170 *F Number of extensions: 39600 *F Number of successful extensions: 82 *F Number of sequences better than 10.0: 18 *F length of query: 541 *F length of database: 122,655,632 *F effective HSP length: 18 *F effective length of query: 523 *F effective length of database: 122,634,572 *F effective search space: 64137881156 *F effective search space used: 64137881156 *F T: 0 *F A: 0 *F X1: 6 (11.9 bits) *F X2: 10 (19.8 bits) *F S1: 12 (24.3 bits) *F S2: 17 (34.2 bits) *F _______________________________________________ *F BDGP mailing list *F BDGP@fruitfly.BDGP.Berkeley.EDU *F http://fruitfly.BDGP.Berkeley.EDU/mailman/listinfo/bdgp *F \------------------------------------------------------------------------------- # *U FBrf0133225 *a Whitfield *b E. *t 2000.12.19 *T personal communication to FlyBase *u FlyBase error report on Tue Dec 19 16:05:01 2000. *F From eleanor@ebi.ac.uk Tue Dec 19 16:05:22 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 19 Dec 2000 16:05:22 \+0000 *F Date: Tue, 19 Dec 2000 16:05:01 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 en (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-update@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: error reports, the lazy way! *F Content-Transfer-Encoding: 7bit *F &agr;-Cat *F CG17947 *F Celera is missing N terminal exon (AE002656; AAF45466). *F please compare to: *F Oda et al, J. Cell Biol. 121:1133-1140(1993), D13964; BAA03067 *F RpS6 *F CG10944 *F Celera is missing N-terminal exon (AE003442; AAF46288) and second *F isoform. *F please compare to: *F Stewart and Denell, Mol. Biol. Evol. 10:1041-1047(1993), L02074; *F AAB05982; AAB05983 *F flw *F CG2096 *F Celera is missing N terminal exon (AE003450; AAF46583). *F please compare to: *F Raghaven et al, Curr. Biol. 10:269-272(2000), AJ249214; CAB59732 *F Mlp60A *F CG3220 *F Celera defines too many C-terminal exons, translation is only 92 aa, not *F 486 (AE003462; *F AAF47158). *F please compare to: *F Stronach et al, J. Cell Biol. 134:1179-1195(1996), X91244; CAA62626 *F thanks *F Nellie # *U FBrf0133226 *a Staatz *b W. *t 2000.12.21 *T personal communication to FlyBase *u FlyBase error report for CG4974 on Wed Dec 20 16:07:23 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Dec 21 01:55:31 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 21 Dec 2000 01:55:31 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 20 Dec 2000 16:07:23 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: wstaatz@u.arizona.edu *F Subject: FlyBase error report for CG4974 on Wed Dec 20 16:07:23 2000 *F Content-Length: 350 *F Error report from WILLIAM STAATZ, PH.D. (wstaatz@u.arizona.edu) *F Gene or accession: CG4974 *F Missed gene *F Comments: the gene product is NOT involved in cell adhesion. It is involved in *F signal transduction in the dpp and wg pathways # *U FBrf0133227 *a Roos *b C. *t 2000.12.27 *T personal communication to FlyBase *u FlyBase error report for CG17204 on Wed Dec 27 08:17:19 2000. *F From FlyBase-error@hedgehog.lbl.gov Wed Dec 27 16:17:28 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 27 Dec 2000 16:17:28 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 27 Dec 2000 08:17:19 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: christophe.roos@helsinki.fi *F Subject: FlyBase error report for CG17204 on Wed Dec 27 08:17:19 2000 *F Content-Length: 546 *F Error report from Christophe Roos (christophe.roos@helsinki.fi) *F Gene or accession: CG17204 *F Missed gene *F Comments: The CG17204 (munin, mun) deduced peptide shows a significant local *F similarity *F to the GDNF family receptor alpha (GFRa) precursors. Drosophila seems not to *F have *F any GDNF family factors despite the presence of a receptor with a Ret-like *F intracellular domain and this GFR-like receptor (cf. hugin, hug). Munin is *F expressed during embryogenesis. *F Browser: Mozilla/4.75 en (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133228 *a Whitfield *b E. *t 2001.1.5 *T personal communication to FlyBase *u FlyBase error report for CG5461 on Fri Jan 5 01:07:10 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jan 05 09:07:17 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 5 Jan 2001 09:07:17 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 5 Jan 2001 01:07:11 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5461 on Fri Jan 5 01:07:10 2001 *F Content-Length: 818 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5461 *F Gene annotation error *F Gene CG5461 has incorrect exon/intron structure. *F Comments: bun, CG5461 *F Celera sequence: AE003636; AAF53200, AAF53201 (2 isoforms) *F Celera have annotated a wrong internal exon, when translation is corrected it *F is a *F match to the previously isolated sequence from: *F Treisman et al, Development 121:2835-2845(1995), L42512; AAC41608 *F Celera have annotated 2 isoforms, the longest at 1211 aa (when translation *F corrected) *F is corroborated by Treisman et al, the second isoform is not confirmed by any *F published *F data. Annotation is missing an isoform 224 aa long, this isoforms sequence is *F from *F Treisman et al (L42511; AAC41607). *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133229 *a Halfon *b M.S. *t 2000.12.28 *T personal communication to FlyBase *u FlyBase error report for CG18485 on Thu Dec 28 13:03:30 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Dec 28 21:03:34 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 28 Dec 2000 21:03:34 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 28 Dec 2000 13:03:30 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: halfon@rascal.med.harvard.edu *F Subject: FlyBase error report for CG18485 on Thu Dec 28 13:03:30 2000 *F Content-Length: 469 *F Error report from Marc S. Halfon (halfon@rascal.med.harvard.edu) *F Gene or accession: CG18485 *F Gene annotation error *F Genes CG18485 and CG3375 should be merged. *F Comments: Gene CG18485 Blasts with perfect match to the reported transcript *F for Dof, transcript I. (see accession \#AJ010642). Thus, rather than a *F separate gene, it is the alternative splice form of Dof (CG3375). *F Browser: Mozilla/4.08 (Macintosh; U; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133230 *a Whitfield *b E. *t 2001.1.5 *T personal communication to FlyBase *u FlyBase error report for CG9907 on Fri Jan 5 01:08:14 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jan 05 09:08:20 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 5 Jan 2001 09:08:20 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 5 Jan 2001 01:08:14 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9907 on Fri Jan 5 01:08:14 2001 *F Content-Length: 796 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9907 *F Gene annotation error *F Gene CG9907 has incorrect exon/intron structure. *F Comments: para, CG9907 *F Celera sequence: AE003502; AAF48617 *F Celera have mispredicted a splice site and when corrected translation is a *F match for *F the sequence from Thackeray and Ganetzky, J. Neurosci. 14:2569-2578(1994), *F U26713- *F U26717. Celera have annotated the longest isoform but there are known to be *F 29 isoforms *F having different combinations of optional exons A, B, C, D, E and F. There is *F also *F some evidence of optional exons H and I from sequence similarity to D. *F virilis. I *F have not annotated all these in Swiss-prot, no idea if you want to! *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133231 *a Whitfield *b E. *t 2001.1.5 *T personal communication to FlyBase *u FlyBase error report for CG4167 on Fri Jan 5 01:09:15 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jan 05 09:09:18 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 5 Jan 2001 09:09:18 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 5 Jan 2001 01:09:15 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4167 on Fri Jan 5 01:09:15 2001 *F Content-Length: 526 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4167 *F Gene annotation error *F Gene CG4167 has incorrect exon/intron structure. *F Comments: Hsp67Ba, CG4167 *F Celera sequence: AE003552; AAF50287 *F Celera incorrectly defined an internal exon and read through the splice site *F for the *F C-terminal exon. When translation is corrected it is identical to Ayme and *F Tissieres, *F EMBO J. 4:2949-2954(1985), M26267; AAA28634. *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133232 *a Whitfield *b E. *t 2001.1.5 *T personal communication to FlyBase *u FlyBase error report for CG4190 on Fri Jan 5 01:10:05 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jan 05 09:10:23 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 5 Jan 2001 09:10:23 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 5 Jan 2001 01:10:05 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4190 on Fri Jan 5 01:10:05 2001 *F Content-Length: 472 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4190 *F Gene annotation error *F Gene CG4190 has incorrect exon/intron structure. *F Comments: Hsp67Bc, CG4190 *F Celera sequence: AE003552; AAF50292 *F Celera incorrectly define the C-terminal exon. When translation is corrected *F it is *F identical to Pauli and Tonka, J. Mol. Biol. 198:235-240(1987), X06542; CAA29788. *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133233 *a Whitfield *b E. *t 2001.1.5 *T personal communication to FlyBase *u FlyBase error report for CG5441 on Fri Jan 5 08:23:56 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jan 05 16:24:09 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 5 Jan 2001 16:24:09 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 5 Jan 2001 08:23:56 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5441 on Fri Jan 5 08:23:56 2001 *F Content-Length: 464 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5441 *F Gene annotation error *F Gene CG5441 has incorrect exon/intron structure. *F Comments: dei, CG5441 *F Celera sequence: AE003756; AAF56590 *F Celera translation reads through the splice site of the C-terminal exon. *F Please compare to: *F Armand et al, Mol. Cell. Biol. 14:4145-4154(1994), L33401; AAA28449 *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133234 *a Whitfield *b E. *t 2001.1.5 *T personal communication to FlyBase *u FlyBase error report for CG1771 on Fri Jan 5 08:26:39 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jan 05 16:26:45 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 5 Jan 2001 16:26:45 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 5 Jan 2001 08:26:39 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1771 on Fri Jan 5 08:26:39 2001 *F Content-Length: 436 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1771 *F Gene annotation error *F Gene CG1771 has incorrect exon/intron structure. *F Comments: mew, CG1771. *F Celera sequence: AE003491; AAF48242 *F Celera translation has missed an internal exon, please compare to: *F Wehrli et al, Mech. Dev. 43:21-36(1993), X73975; CAA52155 *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133235 *a Mount *b S. *t 2001.1.8 *T personal communication to FlyBase *u FlyBase error report for CG2082 on Mon Jan 8 13:56:20 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Jan 08 21:56:27 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 Jan 2001 21:56:27 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 8 Jan 2001 13:56:20 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sm193@umail.umd.edu *F Subject: FlyBase error report for CG2082 on Mon Jan 8 13:56:20 2001 *F Content-Length: 723 *F Error report from Steve Mount (sm193@umail.umd.edu) *F Gene or accession: CG2082 *F Gene annotation error *F Gene CG2082 has incorrect exon/intron structure. *F Comments: There is a microexon (9 nt.) not annotated in AE003601.2 *F GI:10727146, but clearly present based on the sequence of AF145604. The exon *F occurs at positions 23,235 through 23,243, and has the sequence GTTATGGAG *F (CTCCATAAC on the complementary strand, which is represented by AE003601.2). *F Prediction of protein sequence AAD38579.1 requires that this microexon be *F accounted for. We note that the microexon encodes amino acids 197-199, VME, *F which do not appear in AAF51958. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133236 *a Ashburner *b M. *t 2001.1.9 *T personal communication to FlyBase *u FlyBase error report for CG14670 on Tue Jan 9 18:19:09 2001. *F From ma11@gen.cam.ac.uk Tue Jan 09 18:19:09 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 Jan 2001 18:19:09 \+0000 *F To: flybase-update@morgan.harvard.edu *F Subject: FlyBase error report for CG14670 *F Cc: ma11@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Tue, 9 Jan 2001 18:18:48 \+0000 *F Content-Length: 295 *F CG14670 *F I have just done a BLAST on this. It shows a reasonable match with a *F series of biotin holocarboxylase synthetase like enzymes but ONLY over *F the C-terminal half; the N terminal matches lots of unrelated proteins. *F Could this be looked at as a possible case of two genes fused ? *F Michael # *U FBrf0133237 *a Hultmark *b D. *t 2001.1.10 *T personal communication to FlyBase *u FlyBase error report for CG4740 on Wed Jan 10 09:40:21 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Jan 10 17:40:32 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Jan 2001 17:40:32 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 Jan 2001 09:40:21 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: dan.hultmark@ucmp.umu.se *F Subject: FlyBase error report for CG4740 on Wed Jan 10 09:40:21 2001 *F Content-Length: 1920 *F Error report from Dan Hultmark (dan.hultmark@ucmp.umu.se) *F Gene or accession: CG4740 *F Missed gene *F Comments: The sequence for the mRNA of this gene, 'AttC', Accession number *F AF322249, was published recently. It shows that there is a mistake in the *F splicing pattern of CG4740. The predicted intron 1 does not exist and intron 2 *F is incorrectly spliced. In addition, the allele sequenced in the genome *F project has an eight base pair deletion, compared to the allele in Canton-S, *F which disrupts the reading frame. Thus, the former allele does probably not *F produce a functional protein. For comparison, the complete Canton-S mRNA *F sequence is given here: *F 1 atcgtcagtc aacagtcagc tgcttagtca agtagatcga aaatcaacag attaatacag *F 61 caagatgagc aaaattgttc tcctaattgt ggtcatcgtt ggcgtacttg gctcccttgc *F 121 ggtggccttg ccccaacgtc cgtataccca gccactgatc tactatcctc ctccaccgac *F 181 gccacccaga atctacagag cacgtcgcca ggtgttgggt ggatcactca catccaatcc *F 241 gagtggcggt gccgatgccc gattggatct aagcaaggcc gttggaactc ccgatcacca *F 301 tgtaattgga caagtgttcg cagcgggcaa cacgcagacc aaaccggtat ccactcccgt *F 361 aaccagcggc gccaccctgg gctacaacaa tcatggacac ggcctggaac tgaccaaaac *F 421 ccatacgccc ggtgtgcggg atagcttcca gcaaacggcc accgccaatt tgttcaacaa *F 481 cggtgttcac aatctggatg ccaaggcatt tgcctcgcag aatcagcttg ccaatggctt *F 541 caagttcgat agaaatggag ctgcactgga ctactcccac gtcaaaggtc atggagctac *F 601 cctgacgcac gccaatattc ccggattggg taagcaattg gagctgggtg ggcgtgccaa *F 661 tctttggcag tcgcaggatc gcaacacaag gctagatctg ggcagcacgg catccaagtg *F 721 gacaagtgga cccttcaagg gccagaccga tctgggtgcc aatctgggac tctcgcacta *F 781 ctttggataa aactatattc ttaaaaatat tttacaataa ctaagtgtta ttcaaaatat *F 841 ttattgacat aaattcaaac gaattttgtt gcttatttca aatgtgttaa aaatatatga *F 901 cttaactcaa aaaaaaaaaa aaaaa *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133238 *a Hultmark *b D. *t 2001.1.10 *T personal communication to FlyBase *u FlyBase error report for CG7629 on Wed Jan 10 09:52:03 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Jan 10 17:52:13 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Jan 2001 17:52:13 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 Jan 2001 09:52:03 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: dan.hultmark@ucmp.umu.se *F Subject: FlyBase error report for CG7629 on Wed Jan 10 09:52:03 2001 *F Content-Length: 1488 *F Error report from Dan Hultmark (dan.hultmark@ucmp.umu.se) *F Gene or accession: CG7629 *F cDNA or EST error *F Comments: The full-length mRNA sequence of this gene, 'AttD', Accession number *F AF322250, was recently published. The predicted splicing pattern for CG7629 is *F confirmed. The sequence (from Canton-S, differs in one position) is given *F below. It was determined by 5'- and 3'-RACE and should therefore be complete. *F 1 actcaaacta agagatagag tgcaagcaga aagacgaaat caaagaatta tataagatgg *F 61 aatgtcaggc ttcaggaaac ccaaagagcg gagcggcaac cgcccaatgc ggagtaaggg *F 121 tcggtgatga tcttgccaat gctcgagccg gagtattcgc ctccactcca ggcgctgggg *F 181 gtccggtcac caagggagtt tatggagcgg tcaacgccaa tggtcatgca ctctcactgc *F 241 agcatggcca catcgagggc gtgggcagca ctaccactgc cgcagcccaa gccaatctct *F 301 tccagagcaa taacgccgct ctgaatgcca ctgcatttca cagtcatagc cgatcgcacg *F 361 atcagtttgg cggaggactc aatttgcaaa ctggaacggg tcaccaggcg gcagtggggg *F 421 tcactagggt tcctcagttc ggcatgaccg ccgtccaggc ttctggcaca gcaaatctgt *F 481 atacctctcc aagtggcaat ctcagcctca acgccaccgg aagtgccaat catcacctca *F 541 ggggaccgat gcgcggcaag tccgatttcg gcaccggagt taacttgcga tataattttt *F 601 aaatccttta tagttttatt gaaactattc atagtcacat ttagtacttg cacgtagcca *F 661 agaaaagaaa caagtgccgt atttatatgc attatatcga agattaaata aaccatgcta *F 721 ttaaaagcgc tttctacttg gtaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133239 *a Hultmark *b D. *t 2001.1.10 *T personal communication to FlyBase *u FlyBase error report for CG10794 on Wed Jan 10 10:04:25 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Jan 10 18:04:31 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Jan 2001 18:04:31 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 Jan 2001 10:04:25 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: dan.hultmark@ucmp.umu.se *F Subject: FlyBase error report for CG10794 on Wed Jan 10 10:04:25 2001 *F Content-Length: 538 *F Error report from Dan Hultmark (dan.hultmark@ucmp.umu.se) *F Gene or accession: CG10794 *F Missed gene *F Comments: The full-length mRNA sequence of this gene, 'DptB', Accession number *F AF322251-AF322252 and AF334181-AF334182, was recently published. The predicted *F splicing pattern for CG10794 is confirmed. The sequence is given below (from *F Canton-S, differs in several positions). It was determined by 5'- and 3'-RACE *F and should therefore be complete. *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133240 *a Levis *b R. *t 2001.1.10 *T personal communication to FlyBase *u FlyBase error report for CG3168 on Wed Jan 10 17:56:05 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Jan 11 01:56:13 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 11 Jan 2001 01:56:13 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 Jan 2001 17:56:05 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG3168 on Wed Jan 10 17:56:05 2001 *F Content-Length: 851 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG3168 *F Gene annotation error *F Gene CG3168 has incorrect exon/intron structure. *F Comments: The annotation for CG3168 in scaffold segment AE003438.2 includes *F only a single transcript, CT10637. The 5' and 3' ends of this transcript are *F at positions 189387 and 181541 of AE003438.2 and these are listed as the *F limits of CG3168. However, there is a long cDNA, GH13883, which has a 5' exon *F from complement 199940-199444 and then splices to position 184371, which is *F within CG3168. The 3' end of this cDNA corresponds to position 180464. Thus *F this cDNA suggests a transcript with a 5' end 10.5 kb upstream and a 3' end *F 1.1 kb downstream of the presently annotated 5' and 3' ends of the gene. *F Browser: Mozilla/4.75 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133241 *a Leventis *b P. *t 2000.12.28 *T personal communication to FlyBase *u FlyBase error report for CG5387 on Thu Dec 28 15:05:58 2000. *F From FlyBase-error@hedgehog.lbl.gov Thu Dec 28 23:06:01 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 28 Dec 2000 23:06:01 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 28 Dec 2000 15:05:58 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: leventis@sickkids.on.ca *F Subject: FlyBase error report for CG5387 on Thu Dec 28 15:05:58 2000 *F Content-Length: 600 *F Error report from Peter Leventis (leventis@sickkids.on.ca) *F Gene or accession: CG5387 *F Gene annotation error *F Gene CG5387 corresponds to AF231134 (FBgn0026195) *F Comments: Not so much of an error as a duplication of information. *F Flybase has gene records for both Cdk5alpha (CG5387 above) and p35-31C, which *F is the regulatory subunit of cdk5. By BLASTN, these are the same, with over *F 99% identity. *F I would suggest that p35-31C links Cdk5alpha, assuming that you agree these *F genes are the same. *F Browser: Mozilla/4.0 (compatible; MSIE 5.5; Windows 98) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133242 *a Robertson *b H.M. *t 2001.1.8 *T personal communication to FlyBase *u FlyBase error report on Mo n Jan 8 14:31:14 2001. *F From hughrobe@life.uiuc.edu Mon Jan 08 20:33:23 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 8 Jan 2001 20:33:23 \+0000 *F Date: Mon, 08 Jan 2001 14:31:14 \-0600 *F From: hughrobe@life.uiuc.edu *F Subject: Re: more annotations *F To: Sima Misra *F cc: Gillian Millburn *F X-Authenticated: *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='10116516--1619001344--1107014221=:90 *F 00' *F Content-Length: 325836 *F Sima, *F . *F . *F Let me make sure you understand what I have done. These are the results from *F a small 600 EST project on a tephritid fruit fly, Rhagoletis suavis, conducte *F d *F by myself and Stewart Berlocher, and the large 20,000 honey bee EST project o *F f *F Gene Robinson's lab described in my email to Michael. The former yielded fou *F r *F unannotated genes, and the latter about twelve, but many more suggestions for *F improvements of existing annotations (it turned out that my previous estimate *F of tens to a hundred unannotated genes was exagerated when I went back *F carefully and tried to reconstruct each of them). We did BLASTX against *F Release 1 Drosophila proteins at E-05 and TBLASTX against the genome at E-06; *F for the ±9000 honey bee contigs and singletons this yielded about 150 TBLASTX *F hits without corresponding BLASTX hits. I've examined all of these carefully *F , *F and the ones I am sending you are the 50 or so that look interesting (others *F are proteins only in release 2, or artifacts). In addition I was sometimes *F curious about large neighboring unannotated regions in the genome and note *F some interesting unannotated genes in them. *F The two files are both text files, however the New Drosophila Genes file is i *F n *F PAUP format and hence can only usefully be viewed with that program (I've *F attached an old Mac version if you don't have it). Each annotation suggestio *F n *F is separated by a series of asterisks, following which is the name of the EST *F or contig and its sequence, so you can do the searches for yourself to see *F what I saw. *F The mRNA and protein FASTA sequences are together in the second file. *F I'm considering doing something similar for the other available insect EST *F projects, that is, the 15,000 Bombyx mori, 6000 Anopheles gambiae, and 1300 *F Aedes aegypti, as well as our own 1200 Manduca sexta ESTs, and perhaps the *F 17,000 Anopheles gambiae GSS sequences available. I wonder if you've already *F heard from the Bombyx mori and mosquito folks in this regard? *F Hugh *F . *F . *F Hugh M. Robertson *F Professor *F Department of Entomology *F University of Illinois at Urbana-Champaign *F 320 Morrill Hall, MC118 *F 505 S. Goodwin *F Urbana, IL 61801 *F Phone 217-333-0489 *F FAX 217-244-3499 *F Email hughrobe@life.uiuc.edu *F www.life.uiuc.edu/robertson/lab.html *F \----------------------------------------------------------------------------- *F \-- *F 'New Drosophila Genes' file *F [FlyBase curator comment: original PAUP version of this 'New Drosophila *F Genes' file is archived. What follows is a version containing the text *F of the file with the genomic and EST sequences that are shown aligned *F in the original file but does not show the actual alignments between *F these sequences.] *F New genes in the Drosophila genome identified by TBLASTX searches with tephri *F tid fly and honey bee ESTs that don't match Drosophila proteins by BLASTX *F Turns out most are really just problems with the current Drosophila annotatio *F n, where N and C-termini are not annotated *F \********R. suavis J3-A2 *F CAAAGTTTAAAAAAAAATCCAGCCTTAATTCCATTATATGTATGTGTTGGGGTTGGAGCGCTCGGTGCAGTTTTCTA *F TACTTTGCGCTTAGCTACACGAAACCCTGATGTAACATGGAATCGTTCATCGAATCCTGAACCTTGGCAAGAATACA *F AAGACAAACAATACAAGTTCTATTCACCGATAAGGGATTACAGCAACATTAAATCCCCAGCCCCTAAATATGAGGAA *F TAATTTAATAAAGATGTATCTTTCGGCTAGAACATTTCGTTAGCTAATGAAATTAAAAAAAAAAAAAAAAAAA *F Matches human and other organism NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT *F (COMPLEX I-MLRQ) at 52% over length of short 80aa protein *F TBLASTX match to Drosophila genome is an unannotated region of a small messy *F scaffold 142000013386032 *F AE002656.1 *F 176729-ATATAGTTCCATTCTGTTTTATTGGATTGAGTAAAGTTAGgtaattttttataaatgtgtttctttctta *F catattaaatacattttaattataacgtagACAAAATGCAAGGTCTTGGTCTGCAAAGTCTTAAAAAAAATCCAGCT *F gtaagttttatgattatattaagttatctgttgattgcataacaaaaatttgttcagTTAATTCCACTTTATGTGTG *F CGTTGGAGCGGGAGCTATTGGAGCCGTCTACTATATGGCTCGACTTGCTACTCGTAATCCCGATGTCACTTGGAATC *F GCACATCAAATCCCGAACCATGGCAAGAGTACAAAGAAAAGCAATACAAGgttagatttgctccttaatattcatat *F tctattttcatattttaatgggccaagaaaagccctcgaccgtggttttgttaataaccaaatttgcttatagatat *F atgtatttttgcaattttttacttttttggcgatttaaccgacatgccataaccattacacatatatatttcacgaa *F tatatactatgttgtagttgtagttatacccgttactcgtagagtaagatgttatactagattcattgaaaagtatg *F taataggtagaaggcagagttttcaccatatgaagcctatatattctcgatcaggatcaatatccgagtcgatctga *F cgctgtccgtccgtctgtatgaatgtcgagatctcaggaactatacaatctagaaggttgagattaagcatacagat *F tctagagaaatacccgcagcgcaagtttgttggcctatgttgccacgcccacaaatcttcaaaaactgccacatttt *F ttcacatttttattagttttttaaattttgattgatttcccaaaaattttattcaccaatatctatcgatatcccag *F acaaatcatgaaatttcgctatggcattttaactagctgaataacgggtatctgatagtcgaggaagtcgactattt *F tgtacagtgatactttttttcacttttattatttgtctcgcaaaacaattcccaataaccgtaaacaccgcctgtta *F acaatctctaaacggagatatttgcgtatatcaactagctaataacaaattattaaaacaccatactcaccattctt *F tatggcagaaaaaatgtatatatatatatatatatgggtaatttaggtaaataggtaaaataagtgaaattaagaaa *F tgatggggtgtcattagtgcgtattaaggaataattcaacccttaaataaaaaggaagagggatatgcttcaatatg *F tagtgattgcgacttaaacgccaatcaaaccctttaattttatcaattttataataatctagttatttaaaactatg *F aaaaaaaaataaagatcacaaaaatgttctaacatttaatattttatagcaattcggacgcaatgcgggagaactta *F tcatttattttgtcgacagtgactaaccagaaatgtagggatcgccagagatttacacagggtctaaaacgttcgct *F tcagataaatttctatcaaattctatcacttcactagaatagtagattctttaaaatgcatgcaaccagttaatgga *F tgccttcctgaccttaaaaattatacatattcttgaaccgagatgaacctcgtaaacctccagggaactattgaagc *F tagaaagttgagactaagcatgtgtatttaagggtcaactacgcagtgaaggtacataagttccattatattaccca *F caagccccgcaaacactcactgttacttaataatgttttaatttttttttgtttttgcctttcaaatttcttttgct *F aggtaaacaattgtttgcgtaagtaatgtaaaactgattgcgatcgttcagtgtcagtttactaaaatctaaaaata *F tttaaatctcttaattgctggtggacttataggtttactgtaattcgtgttttttagatgaaatatataatattata *F atataatataataatataatataataatataatataatataataatataatataatataatataatataataatata *F atataatataatataatataatatataatatattgaatcttgcattttttttcagTTTTATTCGCCTGTGAGGGATT *F ATTCCAAAACTAAGAGTGCTGCCCCAAACTTTGATGAATAAATTACGTTTCCCTAGCAGCTGCAAT *F GH04411.5prime *F TTAG------------------------------------------------------------ACAAAATGCAAGG *F TCTTGGTCTGCAAAGTCTTAAAAAAAATCCAGCT------------------------------------------- *F \--------------TTAATTCCACTTTATGTGTGCGTTGGAGCGGGAGCTATTGGAGCCGTCTACTATATGGCTCGA *F CTTGCTACTCGTAATCCCGATGTCACTTGGAATCGCACATCAAATCCCGAACCATGGCAAGAGTACAAAGAAAAGCA *F ATACAAG---------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \------------TTTTATTCGCCTGTGAGGGATTATTCCAAAACTAAGAGTGCTGCCCCAAACTTTGATGAATAAAT *F TACGTTTCCCTAGCAGCTGCAATTTAAAAATGTAAAATGAAATAACTTCAAATTATAAATAAACAT *F GM01687.5prime *F ATATAGTTCCATTCTGTTTTATTGGATTGAGTAAAGTTAG------------------------------------- *F \-----------------------ACAAAATGCAAGGTCTTGGTCTGCAAAGTCTTAAAAAAAATCCAGCT------- *F \--------------------------------------------------TTAATTCCACTTTATGTGTGCGTTGGA *F GCGGGA-CTATTGGAGCCGTCTACTATATGGCTCGACTTGCTACTCGTAATCCCGATGTCACTTGGAATCGCACATC *F AAATCCCGAACCATGGCAAGAGTACAAAGAAAAGCAATACAAG---------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \------------------------------------------------TTTTATTCGCCTGTGAGGGATTATTCCAA *F AACTAAGAGTGCTGCCCCAAACTTTGATGAATAAATTACGTTTCCCTAGCAGCTGCAATTTAAAAA *F translation *F M Q G L G L Q S L K K N P A \--------------------------0--------- *F \--------------------- L I P L Y V C V G A G A I G A V Y Y M *F A R L A T R N P D V T W N R T S N P E P W Q E Y K *F E K Q Y K \------------0----very long *F F Y S P V R D Y S K T K S A A P N F D *F E Z *F So, translation is correct, encoding 83aa protein with 56% almost colinear ma *F tch to full-length of mammalian proteins *F Drosphila ESTs indicate that there is an intron in the 5' UTR, no surprise th *F ere. *F All splice sites are predicted, except intron 2 acceptor; but there are sever *F al good acceptors within the long intron that are ignored. *F MQGLGLQSLKKNPALIPLYVCVGAGAIGAVYYMARLATRNPDVTWNRTSNPEPWQEYKEK *F QYKFYSPVRDYSKTKSAAPNFDE *F \*********an extra one! *F The long intron above has several potential ORF-like regions, and *F indeed one matches a short predicted protein, CG13301; *F Score = 45.1 bits (105), Expect = 0.003 Identities = 24/36 (66%), Positives = *F 27/36 (74%) *F So is on reverse strand, about 400bp in: *F AE002656.1 *F ATGCTTAATCTCAACCTTCTAGATTGTATAGTTCCTGAGATCTCGACATTCATACAGACGGACGGACAGCGTCAGAT *F CGACTCGGATATTGATCCTGATCGAGAATATATAGGCTTCATATGGTGAaaactctgccttctacctattacatact *F tttcaatgaatctagtataacatcttactctacgagtaacgggtataactacaactacaacatagtatatattcgtg *F aaatatatatgtgtaatggttatggcatgtcggttaaatcgccaaaaaagtaaaaaattgcaaaaatacatatatct *F ataagcaaatttggttattaacaaaaccacggtcgagggcttttcttggcccattaaaatatgaaaatagaatatga *F atattaaggagcaaatctaac *F translation *F M L N L N L L D C I V P E I S T F I Q T D G Q R *F Q I D S D I D P D R E Y I G F I W \* *F No EST matches for this or CG13301, however these is another something *F out there that is similar. *F Lots of room for a long 5' UTR, and a possible large intron in it. *F \*********R. suavis J3-D1 AGCAGTTCTTCTATGGTGGCTGTCAAGGAAATGATAATCGCTTTGACACCA *F AGGAAGAGTGTGAGAAAACATGCCTTTAAGTGATGCGATCAAGTTTTATTGATAAATTATATCGATAAATTTATCGT *F CGTTGGATTGAAGTCGAGGCATTTAGTTGTTGATGGCCTTTTGTAAATATGTGTTGTACCTTATGAAAATGTATTTG *F TTAAAAAATTATTGTATTATCTCCGTTAAAAACTGTGCATACTAAAATATAATTTATAAAATTTTAAAAAAAAAAAA *F AAAAAA *F Matches various Kunitz domains; best Drosophila genome match is an *F unannotated region of about 22kb! *F AE003623.1 *F aaaccactatggtggttcgcatcgagctttgccgtggttgttgttcgatgattttgggtatagcacacggcatgaag *F ttatatcgagacttaattaagtgccgggtccagaggacctaattcaactgggtcctggagctggaatggaaaatgat *F tgtacggcggttactaacaaattctaacggatatgggttttgtgtaacaaactgctagttgatttttgggttttaca *F tttaaatggaaaggaaaaaaaaaagaaaacaaatttaggtgctggaaaaacgtattttaagcaccactctgtgtaac *F ccaatattttctatggctttatctacatctcacacctgaaaatgattaatttaaaaatttcaatgtacttcgatata *F atctaggcgttcctcatcctccgctaaatatcaccattaatgtgcaataaagaattatgtagagacagttaataccc *F gcgagacaaaaagcggctggcttatcttagccaccaccccgaaattcgcgtgcaaaacaattttcaactcgcttcct *F tttggctgacgacgaaattttcgggatataccccgcctattcaggcatttcccccacattctgtctgccaatttacg *F ttatcgttttcaaattgaaaatttgcgtaatcacattttaagtgaaaatcgctttgcaaattaaattaaatcgattt *F tttcaagacgataaaatcgggctgagtgaaaatcgttctaccaaaagttgcagggcacgtaaatatacattatggcc *F ttctttttatttccgtttccggtttggcattagagctttaagctctgaaatatgcatctgcagttttactttggtgt *F ctgcatagtcgcgacattgcatctcaaagacccatcaacgtcatcaaatcgttagtcaaatacgagaaaagaaaaaa *F tatttaagttctttgctcttcgcgcaagtctctcgtggcaataaaaatgatgaagtactttgaaagtactagtagga *F ctgggctggaaaaataagagaagaagttagaaaagcttgcattcctggcatatcctttagtttttatgcatacccaa *F caatgaaaaaaagggtacattgagatttcgaacgatttgaatgattaagagtagttttgattcttatgatttaaaat *F aaataatagataaataaatttaaaaaaatcatttctagtaattcagctgatgtgaaatattaaaatatatttcacat *F attattctgtcatactagccttgaactttcattaaatgtaaacaaaacaatttataacttgtgtccgtaattttcaa *F atatttttatcATGTATATTTATTTTCCAACAATCTTTCTCTTATTTTTGTATCCAGTAGTAGCAGTTGTCCCTCAA *F GGATTTACAATTAAACAACgtaggtcttagaagaaccaaaaattgcatatgaaatttaaaattgtatatattcttag *F CAAAATGCTGGTATGTGGCAAACCCTGGACCCTGTGATGATTTTGTAAAAGTCTGGGGCTACGATTATTTGACTAAT *F CGTTGCATTTTCTTTTATTATGGAGGCTGTGGTGGAAATCCAAATCGATTTTATACGAAAGAGGAGTGCTTGAAAAC *F ATGCCGTGTGTACAGACCTCCAAATCgtaagaaaagggaagaaaatttggacgaagaagaggaggaagagtttgagg *F aagatattgacaactgggacaaatgggacagcgaatgggacaggatggatctatgaccatatcaatactaagggtat *F tcaagacccgacatgccgaatagacattggcttcaagttaacttttgattcgttgtgcaaacgcataatttcagttt *F gaaagaaagaacttcccgtcgagttggttgtaatgcgttttcttttagccgtctccactgcattttccatcttactt *F acgacggatatatatatatatccctctagACGTCTGTTTGCTGCCAATCTGGGCGACGGCCATTAAGTCAAACCGTT *F TGAAGCAATTTGAAAGCTACCCAGACTATGCAACATATATATTTTTACAGACTCCCTGGGTTATTTATCAACAATTT *F TATGTGGATAGCGTTGCGATTCTGACAATTTTTGACATGCAATTTGCCATTTTCCATCTGCTTCAGCCGTATTTTGG *F GTGTGGAATTTGGCATTTTTCCGCAGGCTGCAATAAGTTTTGGCAGCGAATGCAGATGAGGCTCAT *F translation *F M Y I Y F P T I F L L F L Y P V V A V V P Q G F T I *F K Q \--------------------------2-------------------------------P K C W *F Y V A N P G P C D D F V K V W G Y D Y L T N R C I F *F F Y Y G G C G G N P N R F Y T K E E C L K T C R V Y *F R P P N \-----1--------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \-----------------H V C L L P I W A T A I K S N R L K Q F *F E S Y P D Y A T Y I F L Q T P W V I Y Q Q F Y V D S *F V A I L T I F D M Q F A I F H L L Q P Y F G C G I W *F H F S A G C N K F W Q R M Q M R L M M R Z *F Can reconstruct a nice small gene with two introns, encoding 180aa *F protein with a single Kunitz domain in it. *F MYIYFPTIFLLFLYPVVAVVPQGFTIKQPKCWYVANPGPCDDFVKVWGYDYLTNRCIFFYYGGCGGNPNRFYTKEEC *F LKTCRVYRPPNHVCLLPIWATAIKSNRLKQFESYPDYATYIFLQTPWVIYQQFYVDSVAILTIFDMQFAIFHLLQPY *F FGCGIWHFSAGCNKFWQRMQMRLMMRZ *F Kunitz domain C G C WYYD C F YGGC GN N F T C *F E C *F Best BLASTP match is to PROTEASE INHIBITOR CARRAPATIN, a 69aa protein *F from ticks; 53% over 53aa. *F Neighboring genes are TOLL-4 and Or30a! *F No other BLASTX matches for this 3000bp region, although the entire *F region between the neighbouring genes is 22,000bp, so could be others *F in it, or long 5' UTR introns. *F No ESTs to help solve this one, even from the entire 22kb! *F \***********R. suavis J3-A7 *F ATCAGTTTACGTGGAAGCAATCAAATATTTGCCGAAAATAAGCGTTGATGTTTTAACTAATTACAGTCATTTTGATT *F GTTTCCAAACAAACAAAAAACAGCGAAATGATTGAAGTTACAGATTTACAAAAAATCGGTATTGGGCCTGGCCTGGA *F TTTGGTGTCTCATTCCTATTTTTGGGTGTTCTATTTCTGTTCGACAAAGGATTGCCTGGCCATAGGAAACTTGCTCT *F TCCTAAGTGGTCTGGCATGTGTAATTGGAGTTCAACGAACATTCAGGTTTTTCTTTCAACGGCACAAAGTTAAAGGT *F ACCACAGCATTTTTTGGCGGAATTTTTGTGGTTCTACTGGGATTTCCAATGATCGGAATGGTTATTGAGTTGTATGG *F ATTTTTTGTGCTGTTCAGCGGATTTTTCCCCGTGGCGGTAAATTTCTTGGGGCGAGTACCGGTGCTGGGATCAATTT *F TGAATACTCCATTGATTCAAAAGCTGGTACAAAAACTCGGTGGGGACGCGAATCGAACAACGGTATAGTAGAGATCC *F AAACAAACTTTCAACTAAGGATAATTTTAATTTAGTTTATTCCTCCGTGAAATTAAACTTAAAGCGCGTTTTTGTAC *F AAAAATACATAGATTCCACTTTCACATGA *F Encodes complete protein with excellent matches to 180aa proteins from *F all eukaryotes; 50% identity to CGI-141 protein Homo sapiens *F AE003501.2 RC *F atacgtattaaatggtaaaaatagttttatttaatacattttatcatttatttgcatccaaatggactgaatccaag *F aggttttaaataattaatcggcagcttgatcgctttacaacactaaagcgcttgcatccctgcaaacattgtttaca *F gtcgttagcacgtaactttgaatgaaagtcgaaatcagctgtttgtgctttgaaattgaattggtgtttacgtggat *F ttaattttttctgaaatcaaattaaagcagagccaaatacaaaATGATTGAAATATCAGATTTGCAGAgtaagtagc *F cattaatatgcgaaagccatcagcgcaactaaaccgcctattgaaatcttcccgcagAAATTGGCATCGGCTTGGCT *F GGTTTTGGCATTTTCTTTTTGTTTCTCGGCATGCTGCTGCTGTTCGATAAAGGACTGCTCGCCATTGGCAATgtacg *F ttacccacatgcacatgcaccatatgccccatatagcacacccttcgagctctataataatagcaatcgcctctttc *F ccgcagATTCTATTCATATCGGGCCTGGCCTGCGTCATTGGCGTGGAGCGCACGATGCGCTTTTTCTTCCAACGGCA *F CAAAGTCAAAGGCACAACGGCCTTCTTAGGGGGAATCGTCATCGTCCTGCTGGGATTCCCCATCTTCGGCATGATTA *F TTGAATCCTATGGATTTTTCGCACTCTTCAGgttcgtagcaccctggccaagtcccagtcggattcgtttaagacca *F tttgagcggggctaaccggttacgcactccacatggtcttttcgctttcccgctcttaatacaccattcgcaagtca *F gggcttggcagtcaaagttgctgtcagatgacatcctttagaaatattttatattttaattgaaagactgcaagtca *F tgtagatgggacaatttgacgctgtggcattaacagcaaataccaagtataattcttcagattcgcttacaaaaaaa *F gctcgatcagattttatagcatttgatcagagctaaagaaagcaaaaagtatgtccttcattataactattcgctgt *F ttctgtctgttttgttctgttctgtccttttatacattttttcctatctatacctctctccttttaccttttcaatt *F gcatgcttcttcataagttacgaccaaaggtgcaacttaataattaccctaatatatgtaggtctttttattcatta *F aaatatcttttaacgaacgttaaaaggtctgcttcatatatatcggattaatgaatggaaatctattcaaagtaggg *F aaaatattcagtgaatataagaatttaacttaacttcgaaacgtgcaagatggcaatacaagtggaagtaacttctt *F ttagtgatttgtcgtttacttattacttattaatgtccttctctttatattttcagCGGCTTCTTCCCCGTGGCCAT *F TAATTTCCTAGGCCGAGTGCCTGTTTTAGGATCGCTGTTTAATTTACCATTTATACAAAAGgtaaggtagcatggag *F cctcacaaaaaataaaaaatataacatagcaaatagcccatactcatgatcacttaacgcgctcatctcgccttgac *F gcgaaagcccattgaaataaactgaagggtccacattcccaaacgaccacgcagactcgatttcatagccaattttg *F gtatttatctaagctatgtatttcaacatttacaagtccaaagtagagggatttagtggcgcgtttagtggatcttt *F tcccagtcctcacgctcctcgcgctccttcacgacctccttgaggtagggttccagatacttgacgtcctcctcgta *F cttggtccactgctccttgggcagaatggtcttggtcatggacagatggagggccctcatgatgcggtagttacgct *F catcgtacagcttcctgggcaatcggcgcacggcctccttcacatcctcgttctcatacagacaatcatcgcgatgc *F agacctgcaaaaatcgatggcatcaagctcagctgttaggaatcactaggttatatagtagtcctaccgtattggtt *F gaatccggagagattgtaggcccatctgcccagatttgctgtggggaaaaattcgcattacatatttacgtaatcat *F aatattccggcgtactgcactacgaaatttgttgcatgcaacgtgagtccgattgttgcccgttcg *F translation *F M I E I S D L Q \--------------------------------1-------------------- *F \-------------K I G I G L A G F G I F F L F L G M L L L F *F D K G L L A I G N \----------------------------------------------0-- *F \--------------------------------------- I L F I S G L A C V I G V *F E R T M R F F F Q R H K V K G T T A F L G G I V I *F V L L G F P I F G M I I E S Y G F F A L F S--------2---- *F \--------- *F G F F P V A I N F L G R V P V L G S L F N *F L P F I Q K \---------0---------- *F Can deduce great little gene encoding 140aa protein *F Drosophila protein MIEISDLQKIGIGL-AGFGIFFLFLGMLLLFDKGLLAIGNILFISGLACVIG *F VERTMRFFFQRHKVKGTTAFLGGIVIVLLGFPIFGMIIESYGFFALFSGFFPVAINFLGRVPVLGSLFNLPFIQKIV *F QKLGGDGNRTTV *F J3-A7 MIEVTDLQKIGIGPGLDLVSHSYFWVFYFCSTKDCLAIGNLLFLSGLACVIG *F VQRTFRFFFQRHKVKGTTAFFGGIFVVLLGFPMIGMVIELYGFFVLFSGFFPVAVNFLGRVPVLGSILNTPLIQKLV *F QKLGGDANRTTV *F The region shown here is the RC strand between the forward strand eas *F and cas genes, with CG3560 being in the long 4th intron of this gene, *F on the forward strand \- and it is a real gene! *F No ESTs to help out with this one; although there are several for *F CG3560, which is a component of the cytochromes *F \***********R. suavis J3-B3 *F ATTGGCAGAACTGAATGTTGAAACAACGAATCAAGATGGACGTGTAAATCAATTAGTGAGTGCTTCGCAATACAAAG *F CAGGCATCTACAAGTTGCATTTCGATGTGGCATCATATAACGCAGAACGTGGAGTTAAAAGTTTTTATCCTTTTATT *F GAGATTGTCGTACAATGTGAACGGAATCAACATTATCATATTCCATTACTGTTAAATCCGTTTGGTTACACAACCTA *F TAGGGGTACTTGAATGTTAAGCTGATTAATGAATTATTAAACTAATATTTGAACACACATGGAAATAAATGATGCTT *F TTATATATAAAAAAAACATACCGTGACTCAAACAATGATAACGTGTAGATTTTTTTTTAAATATTCGTTATGTTTTT *F CGAGCTAAACTTTTTTTGTTATTTTTCTATAAAAACACCGCCCAAGCTTTGATCAGAAACATATTAACCACAGTTTC *F AAACCTTATCTAAAAATCATAAAAATTCAAGAAATTTTCATCGAAATCTAAACTGTGCGGAATGGTAGTGCGCACTT *F CCAACTACATCACAGCGCTCAGCACTTGTTAGTTATATGCATGAAAAAAGCAAACGA *F possible end of ORF encodes matches to end of yeast and C. elegans *F 120aa proteins; called probable transthyretin precursor \- fission yeast *F gcaggttgtgtgaccagatcctattaattacgctgttactaataccaaagacac *F tagaataatagaatattctagtgtctttgctaatactttaaacaaatactgaaacatacggcatcaaacgattttta *F tatttttaaaaactgttgcaaatttattcgtttatttggaattaatacttcattaaaaaaaagtatatatatgatcg *F ttccaatttatttccccaaacagatttttggcttacatattattttgatatcccacttatcagtgattttcattgtt *F aatgcaagatgcaatcaaagattaagataaagagtcccataaactggagcttttgctaccgtctgagataccagaga *F tatctacgatctttaatcttaaagttgccctcaagATGGATGCACGAAAGTTTTCTACCCACATATTGgtaactttg *F tttcaatttatttatatgtataaaatttttcgttaaccttttacagGATACTTCGGTGGGAAAGGCGGCAGCCAATG *F TGAGAGTAACAGTTTCCAGGCTGGACGAGATTCAGGAATGGAGATCCCTTCGGGCGGCCCAAACTGATGCGGATGGT *F CGCTGCCTGCTCTTGGAACCTGGTCAATTTCCCGGCGGGATCTATAAGCTGACCTTTCACGTGGGCGCCTATTACGC *F GGAGCGCAATGTGAGGACACTTTATCCAGCAATTGACTTGATTGTGGATTGCAGTGAGAATCAGAACTATCACATTC *F CTTTGTTACTCAATCCCTTTGGGTATTCCACATATCGTGGAACATAGCTCGGTTAAAACCGAATAATGGATGTTACA *F CAACTTACAAAAATGTAATTGATTTGAATAAAGGTTTTTTAAATGTTTATTTGTAACATCTCGGGATCGCTTTACAC *F TTCGTGCGATGTTCGTGCATGAGTAACCGTGTCATGAAATCAACTAACCTCACGCTCCC *F translation *F M D A R K F S T H I L \--------------------------0------------------ *F \---------- D T S V G K A A A N V R V T V S R L D E I Q *F E W R S L R A A Q T D A D G R C L L L E P G Q F P G *F G I Y K L T F H V G A Y Y A E R N V R T L Y P A I D *F L I V D C S E N Q N Y H I P L L L N P F G Y S T Y *F R G T \* *F Drosophila protein *F MDARKFSTHILDTSVGKAAANVRVTVSRLDEIQEWRSLRAAQTDADGRCLL-LEPGQFPGGIYKLTFHVGAYYAERN *F VRTLYPAIDLIVDCSENQNYHIPLLLNPFGYSTYRGT *F J3B3 LAELNVETTNQDGRVNQLVS *F ASQYKAGIYKLHFDVASYNAERGVKSFYPFIEIVVQCERNQHYHIPLLLNPFGYTTYRGT *F No ESTs for this one *F \*************A. mellifera Contig1006 *F GGAAAACATACGTGGAGTCGACGGTTACTACTGGGATCCAGATCTATACGTCAGAGACGTTGAAGCAAGCCGAAGGT *F GTGGTGAGCACAGTGAGGTGCGAGGGCAGGGAACATGCCCTCGGCCGCGGAATCAAGCGAAAGCTGGACTCCATCCA *F TTCCATGCATTCTACCCTGCATGAAGACCAAGATGTAGCCGAGGCAAAGTCGGAGGAGAAGAGCCAGAGGAAACTGG *F AGGTGGGTGAGCTAGTATGGGGCGCCGCAAGAGGAAGTCCGGCGTGGCCGGGCAAGGTCGAGTCTTTGGGCCCACCG *F GGCACCATGACGGTGTGGGTCCGTTGGTACGGGGGCGGGGGCGGTCGGAGCCAGGTCGAGGTCAAGGCTCTCAAGTC *F CCTCTCCGAAGGCCTCGAGGCGCACCACCGTGCGCGAAAAAAGTTTAGGAAAAGTCGTAAATTGAACATGCAGCTGG *F AGAACGCTATACAGGAGGCGATGGCTGAGCTGGACAAGGTGACGGAGTCGAGCAAGGAGCAGAAGGTCGGCGGGAAG *F TCGTGCAAGGTGTCGAGCGGAAGCAAGGAGTGCGGCAACGCAGGCTCGAAGCAGGATGGGAAGAGGTCGTCATCGAA *F GAAGGCGTCTGTCGGTTCTGTGAATCCTGTCGCGGCTGAACAGAAACAGTGTCGGTGATCCGTGTCGATGGATCCTC *F GTACCAGTCAATTTGATAGATCACGATGATGATGAAACGCACTGTTCCATCTGTGAACCATAATCAAACGTGTTAAT *F TTTGTGATTGAGAAACACCGACACCGAAAAACTTCCTCCACAACTCTTGTTTTTTAAAATTCTCGACTGACACGTAC *F ACACACACATGCGCATACATATATACATATACACACGGTGTTTCCCTTGATAACAGAAAAAAA *F Matches DNA cytosine-5 methyltransferase- mammal 40%; Dros EST 51%; *F Dros genome 37, 67%; seems to be in same region, but not same *F translation, as sba gene? *F So this one is tricky. First, it matches the extreme end of a 1498aa *F human protein KIAA1461, in a region known as a PWWP domain in CDD *F However in other DNA cytosine-5 methyltransferases this domain is *F around 300aa in 900aa proteins. Since our best match is clearly *F KIAA1461, and ours is clearly at the end of an ORF, *F try searching Drosophila genome with this protein. Hopeless, since get *F lots of matches, including to sba gene. So could represent an *F alternative end to sba gene. *F \***********A. mellifera Contig1287 *F CCAAGATTGAGAAGGCAGACGTCCAACTCGAGCTTGGACAACGTCGCGCTCAAGCAAATTTTACATTCCAGCGAGAA *F CGTCAATTCGGAAGGTGACACGTCCAAATTGGCCAGCTTCGCGAATCTGAGCAGGCAAAGCTCGGAGAAGGGGATCA *F ACTTGACGTACACGGAACAGGATCGAGATGACGGGAAATCGAATATGTCCGGTAAGAAGTTTGGCCAGACGAATGGT *F AATGGGAACGGTAATGAGAAGAAGACTACGTTCGCCACTCTGCCGAACACGACCACGTGGCAACAGCAGAGCAGCCA *F GCAATCCCAACAGGTGGAACAACATTCTGTTGATGAAAACGGTGGTAACACCATTATGGCCTCGCAACTGAATAACA *F TTAGATTGAAGCTGGAGGAGAAACGTCGGCACATAGAGAACGAGAAGAGGAGGATGGAGGTCGTGATGTCGAAACAG *F CGTCAAAAAGTTGGCAAGGCTGCGTTCCTGCAAGCTGTCACGAAGGGTAAGGTTAAATCTCCCTCTTCATCAACGTC *F TGGGGGGGACAGTCCGGCTGAAATTGGTCCCCCCACTTCTGTAACCTCCGGATCTTCGGGGGAGACCCCGACAAGTG *F TTTCCGAGACGACCCCTGTAACCCAACAACCCTCTCAAGAAAAACCACAGAGACCCTTCTCGCTCAAGGAAATTAGT *F GAAGATGTTCGAGATGTTGAACATAAATGGTTGGAACATGACGGAAATGCGCCATTTATTGAAACAAGACGTACTCC *F AGATATTGAGAACATGGATATTGAACAGTATCATCAATCCATATCACAAATGAATAACAGTCTTAGTGAAATTCAAG *F CTGACATACAACGTTTAGCAAATCGAGCAAATCAAATACAACAACAGCATCTAATGACCCAACACCAACAA *F complete ORF encodes 13% serine and 12% glutamine protein >300aa; *F BLASTX is to N-terminus of 856aa KIAA1078 protein Homo sapiens; *F 24% over 260aa and 5e-10; genomic match links CG18462, CG18459, and *F CG18460!; three ESTs, but not very useful *F So, this tripartite gene spans 10kb, and is probably beyond my *F abilities to reconstruct. Instead suggest they search with KIAA1078 *F protein and see how it spans these three genes. *F \**********A. mellifera Contig1312 *F AAAATCTTAAATTTTGGTTAGACATGTAATAAGTATGGTTTTTGCATTATTCTTTCACATATCTTCTTGTTTTTATT *F AATATCTTTAATAGTTTTTTAAAACTCTGTAAATACATATTCTTAAGATATTTAAAGTAATATCAAGTGTATATTTA *F TATATCTGATGTAAGATACAGGTTATAATTTTGAGATTATTAATACAATGGATTTATCAAAAATTCCAAATGATAAA *F AAATTATATCTTTGCAAATGGTATTTTAGAGCTGGATTTGTTTTTCTACCATTTCTTTGGGCTGTGAATGCTATTTG *F GTTTGCAAAAGAAGCTTTCGTTGAACCACATTATGAGGAACAAAAACAAATTAAAAGATATGTAATATTTTCTGCAA *F TTGGAGCAGCTATATGGTCAGCTGCTCTTTTAGCATGGATTGTTACATTTCAAACACAAAGAGCAGCATGGGGTGAA *F TTTGCAGACTCTATTAGTTACATAATTCCAACTGGCATTCCTTGATGTTAAAATATATATCTTTGTATATTAATAAG *F TGATTATTATAATATAAATTTTTATTGTACGAATTAAAATGAAATAAGACTATTCTTTGTCTTGGTATTTAATTAAA *F TAATAATTGTCTTTAAGATGTAAAAAATATATTATTTATTTAGATGTATTGATTTTAAATAAGATTAAAATTTGATA *F AAACATTCAAATTTTAATTAATTAAATATAATTGAACATAAAATGTAATATTTTATACAATTTATATCATTTATAAA *F AATTTAAGTTACAAATAAATATTTAAGAACTTAAAAAAAAAAAAAAAAAAAAAAAAGCAAC *F Could have internal ORF of 300bp, encoding 14% alanine 100aa protein; *F indeed excellent BLASTX to uncharacterized hematopoietic *F stem/progenitor cells protein MDS033 from human and another 100aa *F protein from C. elegans, 40% and e-18; *F NEW GENE in unannotated region; sadly no ESTs, but will be easy to *F annotate. *F AE003800.2 *F 204127-ATGGACATCTCAAAGGCACCAAATCCGCGAAAACTGGAGCTGTGTCGCAAATACTTCTTTGgtaagagtt *F actaccaatgagtaatgattggattttaaccaagttactttctatttgtctcgaacttagCTGGCTTTGCATTTCTG *F CCCTTTGTGTGGGCCATTAACGTTTGCTGGTTTTTCACGGAGGCCTTCCATAAGCCACCATTTTCGGAGCAGAGCCA *F AATAAAGAGATgtaagtcaatatatgaatagatgcccatgccatacagtctaatattccacaatttctttcctacct *F tcctccagATGTTATATACTCTGCAGTGGGGACTCTATTCTGGCTGATAGTACTAACTGCCTGGATAATAATATTCC *F AGACAAATCGCACAGCCTGGGGCGCCACAGCGGACTATATGAGCTTCATCATACCCCTAGGCAGTGCATAGACATAA *F CTAGATTAATTCGTTAGCA *F translation *F M D I S K A P N P R K L E L C R K Y F F \----------------- *F \-------------------1--------------------------------A G F A F L P F V *F W A I N V C W F F T E A F H K P P F S E Q S Q I K *F R \----------------------------------------1--------------------------------- *F Y V I Y S A V G T L F W L I V L T A W I I I F Q T N *F R T A W G A T A D Y M S F I I P L G S A \* *F bee *F MDLSKIPNDKKLYLCKWYFRAGFVFLPFLWAVNAIWFAKEAFVEPHYEEQKQIKRYVIFSAIGAAIWSAALLAWIVT *F FQTQRAAWGEFADSISYIIPTGIP *F fly *F MDISKAPNPRKLELCRKYFFAGFAFLPFVWAINVCWFFTEAFHKPPFSEQSQIKRYVIYSAVGTLFWLIVLTAWIII *F FQTNRTAWGATADYMSFIIPLGSA *F Neat little two phase 1 intron gene. *F \***********A. mellifera Contig1411 *F ACGTTTTCGTGTGTAATCAGTGAAATTTTTGTGAAAATGTTTTCCACTTCTACACTGAGTGTCCTATTAGGGACAAT *F TTTACTTATCTCTTCGATTCCCGATGCAGTATCTTTTAGCAAGTACGGGAGGACGTGCAAGGACATCGGTTGCATGA *F GGGATGAGGTCTGCGTGATGGCCGAGGATCCTTGTTCGATCTACCAACGAGATAACTGCGGTCGTTATCCGACTTGT *F ATGAAATCTCGTCCAGGCGAGGCTAATTGTGCCAGCACTCTGTGCGGTGAAAACGAATACTGCAAAACCGAGAATGG *F CGTCCCAACATGTGTGAAGAAATCAGCAGTAAATGGATTCGAGTCGGCGGGCGTTTCTTACGTGAACGGGCAGCGGG *F TGAACACGGACGAGAAGCAGCAGCTCGATAAGACGACGGCCAGCAACAGCGCTAGCAATTCGAACCCTTACGCCAAT *F GCTAATGCGCCACCTGCCCCCGCCGAGCCAGCGGGAGGGTATCGCCATCAAGTGAATTCCGCCACCAATTTGGGTTA *F TCCACCTTATCCTAGCTCCGACACGGAGCGTTCCAAGAGCGGTGGGTATCCATCGTATCCTGCTGGCTCCAGCAACG *F GGTATCCGCCTTATCCTTCCCCCAATCAAGGGAATCGCCAACAGGATTTAGGATACCCACCGTATCCAACGCACAAC *F AAAATGCCGATGCCCGGACAGTCGAATTATCCCACGTATCCCGGCCAACCGGGCCACTCCAATTA *F complete ORF encodes 250aa protein with 12% serine and 11% proline; *F lots of weak BLASTX matches involving cysteines in the first 100aa, *F hard to say anything about them; genomic match is similar but at 50%; *F then there are a bunch of ESTs, including two B.mori, and to same *F sequences as Genomic, so is a real gene; NEW GENE in unannotated region *F TFSCVISEIFVKMFSTSTLSVLLGTILLISSIPDAVSFSKYGRTCKDIGCMRDEVCVMAEDPCSIYQRDNCGRYPTC *F MKSRPGEANCASTLCGENEYCKTENGVPTCVKKSAVNGFESAGVSYVNGQRVNTDEKQQLDKTTASNSASNSNPYAN *F ANAPPAPAEPAGGYRHQVNSATNLGYPPYPSSDTERSKSGGYPSYPAGSSNGYPPYPSPNQGNRQQDLGYPPYPTHN *F KMPMPGQSNYPTYPGQPGHSN *F TTCCCGCGGGCGAAGAGTACGGCCGTGGATGCGGGGACATTGGCTGCCTGCCCA *F CCGAGGAGTGCGTCATCACCAGCGACTCGTGCAGCTACAACCAGCGTGACGGCAAGGATTGCGGCAACTATCCCACC *F TGCAAACGGCGCTCCGGCGGAGGATCATCCGCCTCGAACAGCAGCCCCAACTTGGCAGCCCCCTCGGCCAATCCGTC *F AGGTATTTAGCTTGGAACCCCCCACTTGATTActcattgtgcgcttccctgggggagttgtccaggcgattggcatc *F gtgtttcgtaattcgaatttcgaggtttggcgaccggcgattggcggctacatctttttggttcctcaaaacaagtt *F atttcgggttaaatccaacgaatttctgggggcgcaaaagctgacttcgggcgccgaagataacaatagcctctcag *F agacgagaaataacacgttgcattgaatacaattcaagaaataataattttctgaaatatacaaataatataacgct *F attgactgacctattgtcctaataatcacatcacgtattcacatactctttccaagcctcgaaactctgcaaggcta *F taagcttaattccaaattaccgaacttatttgtcgtttctttttgtttggccccaagtatttgttaagttttgtgat *F catttccatttatatgtatgtatctatgctatataactgaatgtaatatgttttatggcgatctaatacccacacca *F acacactgagcgcttccgctgactcaatcaatttaacactcaattcgactctcaatcaatcaatcaaccaatcactc *F gctcgctcgctcattcatcgcctcattcgctcaaggcttgcaaatattcgactgctaaccacccacccgcccccgcc *F tctccccctctccaccgatcacttgtgcatgttttaggaagcaacagtacagagagaaaaaaggcaacatgcaacac *F gtctatttacttctgtgtatataacatatatttcaaattcaaattcaactttattagcactaaaaatctttacaact *F gcataatagcttttgtattttaagcttacacaatctagcagctgaaatgaaaataataatttaagagcaaaaccaat *F ttcttaatgtccatgtcttaaaattaacatcaagaaggttacgagtacttgaattaaaatgctagaatcatgttgtt *F aaggaagcttgactggtgcctgacccatcatagattaacatatatat *F translation *F E Y G R G C G D I G C L P T E E C V I T S D S C S Y *F N Q R D G K D C G N Y P T C K R R S G G G S S A S *F 5' region ttgcaagcttgtaatgcgcttttgattggttacatagggcagatgcgttttttt *F ttttgtttagaagcaaactgccttcaaacttgttttaactcttacgcgaaagttggccaactgaaaaaaaagtattt *F ttccatcttgtactttgcagcaacttttgatccaagggcgtgacatcgatagcgagcaacaggatgctggcactgcc *F tttgctgactctggcggtcctcgccagctgcggctactccgtggacgcctactccagtacgtatacgtaatatttca *F ggtgggggttggctttcgtggacaccttaccaccaactattgtcctagaaagccatcaccccactgccatttgttgt *F gttgtgtaatcagtactcctgaaatgagcaccgatcccttggatcggtggtgaacctttcatgccttcatcaaccct *F tgcccctccatcattgacatactgaagccagatgcggtatccccgattttgagcagacttataatttgattttcttt *F tttttttccgtatgattttgacccacccactctgatccacaaaacacacaccgaaacccgcaatccgcaacccgaaa *F tccgaaatccgtaatccgcaacccgaaaccgtaaaccgtaatccgtaatccttgaacctaatcgaat *F This is going to be horrendous, with the ESTs showing a 5' end in the *F next file, about 70kb away with a bunch of gene inbetween! then *F linking CG1735 and CG1726. *F SIMA \- this one is rather complicated, suggesting that there is a huge *F gene linking CG1735 and CG1726, try working with the Bombyx mori ESTs *F identified using ours. *F \***********A. mellifera Contig1463 *F GCAATTATCCTCTTCCTTCCCGTTTTTGTTTTCGTTTTTTTTTCCTTTCTTTCCAGTTCGCTTTTTTTTTTTTTTTC *F ACTTCACTTCTCCCTCCTTGGGCATAAAGGTCAACTCGAAATTGGTTGTTCATTGTTTTTATTGTTTAACTCTCGAT *F CGATCGATCGTTCGTTCGTTCGTTCGTTCGTTCGTTCGTCATGCGTGATTGCGTGCGTACTTAAATGAGTGCGTGCG *F TGCGTATTCACTCACAGACTTGCAGAAGTTAGCTCCATGACTGGTAGGAGTCTTGGTACCATTCGTGATCATCGCTG *F GAAACACCGCCAAGAGTGGCGGTCCTACCTCCGAATCCTATGTCACCGCCGCCACCACCACCACTGCCACCATTCAC *F ACTCGCCAATCCGTACGCATTGCCCAGTGGTTGTTGGGCGAGGGGTTGGTTTCCCCAGTTGCTCTGGAAGCGACGCT *F TAGATTCCCCCTGGTTCTGGTGACCCCCGTCAAATTTTCGTTTACCTGCTGGTAAACTTCCCTTGGCGCGGACCCCC *F CCACGAGCGGACAGCCGCTGGACCCCACGAGCCCCTGAAGTTGCGGGGTTGCGTCCCCCCCTGACTGGCCCACGGAC *F TTGGGGGCCACCGACTCGGCCACGCGGCACTACTCCACGGCCACGTCCAGCCGGTTGAGGCTGCCTGCCTCTCCCTC *F TGGCAGGTGGCGGAGGCGGCGCGTAATCGAAATAGTAATCTTCGTATCGATAATAGTCATCGTAATATGGATCAC *F no obvious ORFs; but in RC end of ORF encodes 21% glycine and 11% *F arginine protein with 35% e-05 match to end of 600aa heterogeneous *F nuclear ribonucleoprotein R Homo sapiens; seems real to me; genomic *F match is to C-terminus of CG17838; provides real end of this protein. *F \***********A. mellifera Contig1481 *F TGTGCTTCCAATTTCTTTTTTCTTTTTTCAATTCTTTTATGCTTTGTACTTTTTAAATGAATGTTCCATTGAAATTC *F TCCAATAAATATCCTATCGCAAATATCACAAAAGTGTCTTTCCTCGTTGCTACTATCACTGAATTTTTTATTCTCTA *F TTGATTCGTTTAAAGGTTTTTGTTCAGGCTTTTCTCCTCTTAGCACAGCTTCGATTATTGCCACAGCTGGTTCATAT *F ACACAACTGTCCCATTGATTCACATCGGTAGAATCTAATACATAAATTGGTGGTACTTGTCTGTCACTGCGACGAAG *F TAGACGATTCATAACCCATTTTTTCTGTTTTTTCGCGTACCTCTTCGTGACCATTTTTAAGTCATCAATACCCCTTT *F GCAATAATTCTTGTCCTTTTTTCCCTCCCTTCTCTTCTTCTGGCAACACAAGGTAATCATGAAACTCTTTGAAGCCG *F ATACTTTGAAAAATGCCCTTCGTATAATCGACTGATGTGTTGGATTTAATCCGTTGCTTGTTGTATCTCCGATGAAA *F GTCAAGCAGTTCCTGAACCAGACCGGTCTCCACCATGTCGTCGACCCTTCTCTCCAACCGATCCTCGAGGACTTTCA *F TGTCACAATTGATCCATAATAGAATGGCATTGCGGTATCTCAAAGGACCTCCTAATCCAGAACCACCAGCTATCCTT *F TGAGCTTTTAGCAATTCTGAATGCTTCACACCATGTTGCTCGAACACTTCAAGTGATCGAATGATCTTCCTCCTATT *F GTTCGGATGAAATCTCTTCGCCATTTCCGGATCCACTTTAACCAACTCTTCGTAAAGCTCCTGGTTATCCTTTGTCA *F TCGATCGATCCAACTCGATCTTCATCCTCTTCGTACGCGACACATTCTCGTCCAACCGGTCATCATCGTCCTTGCCG *F ATCCCCGAGTCGTTCATCAGAACTTCCCAAAGGATGGACTCTATGTAATAGTTGGTGCCACCGACGATGATCGGGAG *F CTTCCTCCTCGCGAGAAGATCGTTGATAATAGGTATGGCAGCATCCCTGAATTGTACCACCGTGTAGCTAGGGTTCA *F GAGGGTCTACGATGTCCAACATGTGGTGAGCCGCCTTTGCTTGTTCCTCTTTCGTTACTTTCGCGGTCACGATGTCG *F AGGCCTTTGTACACCTGCATACTATCGGCTGAAATAATTTCTCCGAAGAATTTACAAGCTAATTCGATGGCCAAAC *F complete ORF in RC encodes tRNA isopentenylpyrophosphate transferase *F Homo sapiens; 47% full-length e-102; *F genomic match is 43%? But single ORF and unannotated! NEW GENE. One *F EST *F Match to human protein misses just 40aa on each end, a 467aa protein *F AE003749.2 TACCAATTACTTGTAAGCACAAAAAACAGCTGACGGCAACAAGTGGTTCGGTCC *F CCATCGGAATACACGTGCTCAAAACGTGTGGGTTTTATTTGCCTTAATTGACTTAAATTCACTCGCAATAAGTGGAA *F ATGATTCGAAAGGTGCCGCTAATTGTAGTCCTGGGCTCCACGGGCACCGGAAAGACGAAACTGTCTTTGCAACTGGC *F CGAACGCTTCGGAGGAGAAATAATCAGCGCTGACTCCATGCAGGTTTACACCCACCTGGACATCGCCACCGCCAAGG *F CAACCAAGGAGGAGCAGTCCCGGGCACGACATCATCTACTGGACGTGGCCACACCGGCCGAACCCTTCACAGTCACT *F CACTTTCGTAACGCAGCACTGCCCATTGTGGAGCGCCTGCTCGCCAAGGACACTTCTCCGATTGTGGTGGGCGGCAC *F GAATTACTACATAGAATCCCTACTTTGGGATATTCTGGTTGACTCGGATGTCAAGCCGGACGAAGGCAAACATTCGG *F GGGAGCATCTTAAGGATGCCGAACTGAATGCTTTGTCCACCCTCGAGCTGCATCAGCACCTTGCCAAGATCGACGCA *F GGTAGTGCCAACCGTATTCACCCCAACAACCGGCGCAAGATCATCCGGGCTATCGAAGTGTATCAGAGCACCGGGCA *F GACTTTGAGCCAGATGCTGGCGGAACAGCGGGCACAGCCGGGAGGAAACCGCCTGGGTGGACCCCTTCGCTATCCAC *F ACATCGTTCTCCTTTGGTTGCGTTGCCAGCAGGATGTTCTAAACGAGCGATTGGATTCCCGCGTAGATGGCATGCTG *F GCCCAAGGGCTGCTCCCTGAACTACGACAGTTTCACAATGCCCACCATGCTACCACTGTGCAAGCCTATACGTCGGG *F AGTTCTGCAGACGATTGGCTACAAGGAGTTTATTCCCTATCTGATCAAGTACGACCAGCAGCAGGACGAAAAGATAG *F AGGAGTACCTCAAAACCCATAGTTACAAGCTGCCAGGCCCAGAAAAACTGAAAGAAGAAGGTCTTCCAGATGGCTTG *F GAACTCCTACGCAATTGTTGCGAAGAACTAAAGTTAGTCACTCGCCGATACTCAAAGAAGCAGCTGAAGTGGATCAA *F CAATCGATTCCTGGCCAGCAAAGATCGTCAAGTGCCGGATCTCTACGAACTGGACACCAGTGATGTGTCAGCTTGGC *F AGGTGGCAGTCTACAAGCGGGCAGAGACCATCATAGAAAGCTATCGAAACGAAGAGGCTTGCGAGATACTACCAATG *F GCCAAGCGGGAGCATCCTGGAGCGGATTTGGATGAGGAGACTAGCCATTTTTGTCAAATATGCGAACGGCATTTCGT *F TGGGGAGTACCAATGGGGACTGCATATGAAGTCCAACAAACACAAGCGAAGAAAGGAGGGACAGCGCAAGCGGCAAA *F GGGATCACGAAACAATGCTCTCAACGGATCTAGCGAAGAAGCAAAAGGAGGAGAAAGAGGAGGCAGGAAAGGCGGAG *F ACTCAGCCACCACCCAGCCGAGTCAATGATACTGATAAGGCAATGtaacactagacgcggcttggcaataaatgaac *F ctacgtaaatttgagtcatttgttgttgttttgaatctcaatcccaccgttttgctgctgatgcaagcggcttgagg *F agtatctgataaccctacacctcgctaatggggaccacagaccgcaggggaggtcgttgcctagccagaaaagcgaa *F aacgcgtaaacatgtttgtgcaccgaacaaccagcccacacaatcgccatcgcccactgactgatctcgtctttcat *F ttgcatttcagttgcccagcggttcagacgcaattagagaaaccaat *F LD10347.5prime *F TACCAATTACTTGTAAGCACAAAAAACAGCTGACGGCAACAAGTGGTTCGGTCCCCATCGGAATACACGTGCTCAAA *F ACGTGTGGGTTTTATTTGCCTTAATTGACTTAAATTCACTCGCAATAAGTGGAAATGATTCGAAAGGTGCCGCTAAT *F TGTAGTCCTGGGCTCCACGGGCACCGGAAAGACGAAACTGTCTTTGCAACTGGCCGAACGCTTCGGAGGAGAAATAA *F TCAGCGCTGACTCCATGCAGGTTTACACCCACCTGGACATCGCCACCGCCAAGGCAACCAAGGAGGAGCAGTCCCGG *F GCACGACATCATCTACTGGACGTGGCCACACCGGCCGAACCCTTCACAGTCACTCACTTTCGTAACGCAGCACTGCC *F CATTGTGGAGCGCCTGCTCGCCAAGGACACTTCTCCGATTGTGGTGGGCGGCACGAATTACTACATAGAATCCCTAC *F TTTGGGATATTCTGGTTGACTCGGATGTCAAGCCGGACGAAGGCAAACATTCGGGGGAGCATCTTAAGGATGCCGAA *F CTGAATGCTTTGTCCACCCTCGAGCTGCATCAGCACCTTGCCAAGATCGACGCAGGTAGTGCCAACCGTATTCACCC *F CAACAACCGGCGCAAGATCATCCGGGCTATCGAAGTGTATCAGAGCACCGGGCAGACTT *F M I R K V P L I V V L G S T G T G K T K L S L Q L A *F E R F G G E I I S A D S M Q V Y T H L D I A T A K *F A T K E E Q S R A R H H L L D V A T P A E P F T V T *F H F R N A A L P I V E R L L A K D T S P I V V G G T *F N Y Y I E S L L W D I L V D S D V K P D E G K H S *F G E H L K D A E L N A L S T L E L H Q H L A K I D A *F G S A N R I H P N N R R K I I R A I E V Y Q S T G Q *F T L S Q M L A E Q R A Q P G G N R L G G P L R Y P *F H I V L L W L R C Q Q D V L N E R L D S R V D G M L *F A Q G L L P E L R Q F H N A H H A T T V Q A Y T S G *F V L Q T I G Y K E F I P Y L I K Y D Q Q Q D E K I *F E E Y L K T H S Y K L P G P E K L K E E G L P D G L *F E L L R N C C E E L K L V T R R Y S K K Q L K W I N *F N R F L A S K D R Q V P D L Y E L D T S D V S A W *F Q V A V Y K R A E T I I E S Y R N E E A C E I L P M *F A K R E H P G A D L D E E T S H F C Q I C E R H F V *F G E Y Q W G L H M K S N K H K R R K E G Q R K R Q *F R D H E T M L S T D L A K K Q K E E K E E A G K A E *F T Q P P P S R V N D T D K A M *F Amazingly is a single ORF! With no obvious 5' intron either. *F \*************A. melifera Contig1578 *F GTTGCTTTTTTTTTCAGAAGTAATTATATTCTGTTATATATAATTACTTCTGAAAAAAAATTTTTCAAAATAACATT *F ACCAAATCAATACATTTTTCTGTTCATTCGCAAACTGAAAATTCATAAAATTCAAAAAATGGGAATGTAAAGAGGCA *F AAATTTATAAATATTTTAAGTATTCAATTAAATAATGTTTTACTTAATTAAAATCATAATCCATATTTTATTATTGA *F TAATTTTTTTTTCACAAGGAGATATCAAATAGATACCTAATTTGTTCACAGGGCACCAAATGGATCAAGTTGAACTT *F GATTATTTTGTGGTTGCGCAATATTCTGCTGTGGTTGCTGCTGTGGTTGTTGTTGAAGGTTAGTACCCATCATCGAA *F TTTGAACTAGACATCATCATTGGTGCAGCTCCTCCTGTGACCATCATGTTACCAGGACCACCAGATATTGTGCTCAT *F CATAGGTCTTATGCCTTGCATACTTTGCATGCCTATTGGCACACCTTGCATTCCCATTGGACGATATCCAGCGCCAG *F TTGTAGCTGCCATAGGTTGCGGTGTCCATCCTCCAGCTGAGCCACCAGTTTTGGCAGCATTTTTAGGCGAATTCCAT *F TGCATACCTTTGACTTGTTGCTGAGCACTTTTGTTGATGGTCAAATTTTGAGCAAGACTAGCAAGACTGCTATCCAA *F ATCTCCAGTCAGGACTTTACCAGTAGACGCTGCATTCTGTTGTTGTCCTGCTACTGAAATCGGTTGCTTTGCCGGGG *F AACCGTACCCCGCCGGGACTTGGGTGGGGATACCGTAGGCCGACCATCGGTCCG *F possible end of ORF in RC encodes 130aa 14%G/Q protein; BLASTX match is *F 39% over 56aa, 5e-05 to clathrin assembly protein AP180 short form \- *F rat; and many others, indeed frog is best match; *F genomic is unannotated within an intron \- NEW GENE ; one EST to *F Anopheles gambiae of all things, but only 43%? *F Turns out is just an alternative C-terminus for the lap gene, with some *F extra exons within the final large intron of annotated gene. *F \*************A. mellifera Contig1637 *F CTGTGCACGTCGCACAGGCGCCAGCTGCTCGTCATGCTGCAGAACCACAACAAGCTGCGCGACATCAGGCGTAGGTG *F CACCAAGGCGAAGGAGGAGCTGTCCGTGAACATCTATCACCGGCTCAAGTGGATCATGTACGTGGAGAACAAGATGA *F TGGAGGTGGACGGCAAGTTGGTCATGTATCACGAGAGCCTGAAACGTCTGAGAAGGCACCTCGAGGTGTTGCAACAG *F ATCCATCTCGCGCCCCAGATGTACATGAACGCCGTGGCCGAGGTCGTTCGTAGGAGAACGTTCTCGCAAGCTTTCCT *F GGTCTGGGCGAGCAACCTGGCCTGCCAATTGCTCACCGTTCACAGCGAGGAATTGGCACGTAGAAGGGAGTTTCAGA *F GCAAATTCGACGGCCACTTCCTCAACACGTTGTTCCCAGGCCTCGAGGACACGCCACCGCCGTTCGCCACCCAGGCG *F CCGTCCGTTTTCGACAACGGATTGCCAAAGTTGACGGCCGAGGATATGGAATCTCTGAGATCTCAGCTACCCGATCT *F GGCGCTCACCATCTCGTCGCCAGATTTGAACAGCATCACCCAGTTCTTCCTGTCCAAGAGTCTCACCAGCACGGACG *F AGAACAACAAGGAGAAGGACGGCGCCTCGATGCGCGTGGAC *F complete ORF encodes at least 130aa 14% leucine protein; full-length *F 39% match at e-23 to KIAA0203 gene product Homo sapiens and C. *F elegans and arabidopsis; *F genomic match is to 46% to region of CG1347; but annotation needs *F fixing it seems \- NEW ANNOTATION; one good EST *F \************A. mellifera Contig1793 *F AGATTCCTAAAATCCTTTCATGGGGCCGCGGCCAGCCGGATGATATCAGTGCGATCAATCTAGGAGATGAGAAATTC *F GACCCTGACTCGGATAAAAAGCCGCGCGCAGGACAAATTCTATGGATCCGTGGTCTAACACGACTACAGACACAGGT *F AATAGGTGGCGAGTTGCAGGAACGTTTGATACCAGTACCCTACAGCAAGAGTTCGACAGACCAAGCTATCCGCGTAG *F TGAACGCATTTCGGCAGGGCCTAGACGCACGCTACACGAGCGAGCACAGCAGCACTACATTGGCGGAGGTACTGAGA *F AAACAGTCGTCCTTAAGCAAGCGGCTCTCGCAAACGAGCAGCATTGAATACGCCGATAACAACCCAGACGAACTGAC *F CATACCCGAGATAGATGTGGAAAGATTGTCAAGTCACAGTCATACAGAGACCGCTGTTTAGAATGGGAGAAGAATGG *F CGGGGTCAGCAGCGATATGTTTATCAAGCTGTGTTACGTTCGATCCTCTCTGACGATATGTAAACACGAAAAAGAAG *F TCATCTTCATCCTTGTTTATCTCGTGGCCAGCGCGTTCGGAGGAAAGGACAAAAAAAAAAAAAATAAATA *F long ORF encodes >130aa serine rich protein; BLASTX says is N-terminus *F of Ca2+-transporting ATPase (EC 3.6.1.38), plasma membrane isoform 1c *F from rat and C. elegans; 40% over 82aa; 4e-04; *F genomic match is 75% to unannotated region \- NEW GENE; several ESTs. *F AE003844.2 aataatatatttatttatttatatccttatattttagGTGGGGTCGCGGACATC *F CCGAGGAGTACACAGATGGTATGAATCTGGGTGAGGAACGCTTTGATTCAATTGATTCTGATAAAAAGCCTAGGGCT *F GGTCAAATTCTATGGATTCGTGGTCTAACTCGCTTGCAAACACAAgtaagtgtcaattcaacaacaacatcaacttg *F tcttaagaaactagaactaaacaataattacctaaaaggatcaacagttatatatcaatttgtttaaataatgactt *F ttttatgttgttgtgatatatttttataaatttctatttctattcattattcacatttattacattgatattttata *F tgatataatgatataattaattattaaataataatataatataatataaatataataaaatattatatatatatata *F tatacacatatatatacctacctactatatagannnnn---nnnnn11kb intron, at least,ttttatgac *F tattttactctttcccacttatattttctgtattactttctatctccctctctttctcttcattcttaaagGTAATA *F GGCGGCGAATTGCAAGAACGCTTGATTCCGGTCCCATATAGCAAGAGCAACACTGATCAAGCTgtaagccttaacaa *F ttttgttttatgttatttttacagttttaaaataagtcgaattattaaattctatctcggcaaaagcgtaactataa *F gaatgaacgatgatatacttgtagtacgtttgtctattcactaagaacacaatttttttaaatcgtctgtttgtccg *F taataataaggtaatgaaaggcaaggcaatttaataggcgtattgtgtgtcaaccacaagcttaatttaatatgccg *F aatttttaacccacctatatccaaaatatatatggttatatttcttttttaattatgatttagttggtttttcgtgc *F ccactggttttgctttaaacttccatcatgtagaagaacgatatacttagttttttaatgtgtttgttcgtcaccac *F ttaaataaaatcaaaaaaaggttgtcgcataatgcatgattaggcaaattaattttagattgctgattaattggtaa *F attaccgagctacagtcctccgaattatgaacaaaataagcgaaatattaaaaagaataagcaactcatatgaagtg *F ttgttactgattatttgtccgtctgaattaattggtatttggatatcccattattaacttaagatctatcttatcat *F ttatgtgtcgcatctgctcgtagtaagaattgtcaaaataatatttgtatttaatttgaactagaaatatacataaa *F agaaagtatgttcatatatgtataattggatctttagcttgaatgattaaagatgttcttcttatactattgtttat *F gtcccttttgtcactgttcttggtgttgttcttttctttttactaaatgtacgcttagATACGAGTGGTAAACGCAT *F TCCGCCAGGGTCTGGACGCCCGTTACGGTGATCACACCAACACATCCCTGGCAGAGGTACTGCGTAAGCAGACTTCG *F TTGAGCAAACGCCTTTCGGAAACGTCTTCCATTGAGTATGCCGATAATATACCTGATGAGCTGACCATACCCGAAAT *F TGATGTCGAACGTCTATCATCCCACAGTCACACTGAAACTGCAGTTTAAATTTCAGTGGCATCCATATCCATATAAA *F AATAAACCGCACACATTCTCAGAAATAACAATTCTAAgaaatccttagcacagcttggaatttttataaaaaaaggt *F tttgttcaggataacagcaatgtagctgtgaattggttaaaaagcattttgtaattcagcaaaaataatccgtaaaa *F aaaatgtaaatttctaattttttttgttagtatgtatgctaacaaaatatataaagtacttaatattaatataattg *F taatgcaagggcatacatacattgattataaccacctttaactcaaaatgtaagcggatcggttttgtctcgcacac *F tgaagccattaattaatattttatcgtcttacatgtaataaatgattcaatgaataaacatgttttatttacttaca *F cgtggaaaaggttagcactataataaatcgaccaaacggtgcaaaagaaacagaaaagcacggatc *F GH15464.5prime *F GTAAACGCAT *F TCCGCCAGGGTCTGGACGCCCGTTACGGTGATCACACCAACACATCCCTGGCAGAGGTACTGCGTAAGCAGACTTCG *F TTGAGCAAACGCCTTTCGGAAACGTCTTCCATTGAGTATGCCGATAATATACCTGATGAGCTGACCATACCCGAAAT *F TGATGTCGAACGTCTATCATCCCACAGTCACACTGAAACTGCAGTTTAAATTTCAGTGGCATCCATATCCATATAAA *F AATAAACCGCACACATTCTCAGAAATAACAATTCTAAGAAATCCTTAGCACAGCTTGGAATTTTTATAAAAAAAGGT *F TTTGTTCAGGATAACAGCAATGTAGCTGTGAATTGGTTAAAAAGCATTTTGTAATTCAGCAAAAATAATCCGTAAAA *F AAAATGTAAATTTCTAATTTTTTTTGTTAGTATGTATGCTAACAAAATATATAAAGTACTTAATATTAATATAATTG *F TAATGCAAGGGCATACATACATTGATTATAACCACCTTTAACTCAAAATGTAAGCGGATCGGTTTTGTCTCGCACAC *F TGAAGCCATTAATTAATATTTTATCGTCTTACATGTAATAAATGATTCA *F LP02848.3prime RC *F ACGCAT *F TCCGCCAGGGTCTGGACGCCCGTTACGGTGATCACACCAACACATCCCTGGCAGAGGTACTGCGTAAGCAGACTTCG *F TTGAGCAAACGCCTTTCGGAAACGTCTTCCATTGAGTATGCCGATAATATACCTGATGAGCTGACCATACCCGAAAT *F TGATGTCGAACGTCTATCATCCCACAGTCACACTGAAACTGCAGTTTAAATTTCAGTGGCATCCATATCCATATAAA *F AATAAACCGCACACATTCTCAGAAATAACAATTCTAAGAAATCCTTAGCACAGCTTGGAATTTTTATAAAAAAAGGT *F TTTGTTCAGGATAACAGCAATGTAGCTGTGAATTGGTTAAAAAGCATTTTGTAATTCAGCAAAAATAATCCGTAAAA *F AAAATGTAAATTTCTAATTTTTTTTGTTAGTATGTATGCTAACAAAATATATAAAGTACTTAATATTAATATAATTG *F TAATGCAAGGGCATACATACATTGATTATAACCACCTTTAACTCAAAATGTAAGCGGATCGGTTTTGTCTCGCACAC *F TGAAGCCATTAATTAATATTTTATCGTCTTACATGTAATAAATGATTCAATGAAT *F translation *F \-------1---------------- W G R G H P E E Y T D G M N L G E *F E R F D S I D S D K K P R A G Q I L W I R G L T R L *F Q T Q \---------------0-------------- *F V I G G E L Q E R L I P V P *F Y S K S N T D Q A \---------------0------------ *F I R V V N A F R Q G L D A R Y G D H *F T N T S L A E V L R K Q T S L S K R L S E T S S I E *F Y A D N I P D E L T I P E I D V E R L S S H S H T E *F T A V \* *F 5'region for 5' exon \- 4kb more available *F attcttaaatctatgtttgaggactatgaccgcgctaatcacctcccgcatggtcatatctcttgacacttcggcaa *F taggccgctgcaatcgtacttatctgtagttgccacttatgctgtccggtgacatgtttattgtagcccataaatag *F aacatttttatagtttgcctacttatcattatggaaatatcgacatggctagtgatcagtatcaataacatacaatt *F aactttatatcgtaggatatgcgtctttctattgccagaattgtttcttttaagtattctatgaatagtaaagggtt *F tattaacctgtagttttatcatatatgataattataccttttactcgtagaggaagcgcttccgacaatataaagta *F tatatatttctgaccaaaacaaccacacccccacttgtcttgccaatatcggtcggtatactaaatacacttttttt *F ttttaatatggctctgtggctgtccaattgattaaatgcgttcagttctcgtctttgaagagtggtttctgttctaa *F aatgatggtcctgatcaagaatatatatacttaatatggtctgaaaagtttccttctgcctgttaaatacttttcaa *F caaatctggtaattcttttactctcgcctaacgggtataattaattagtcatatcgggctactatatcatatagctg *F ccatatagcgatcggtctgcaataaagtgtttgtatggttggcagctgcccttctctggacctaaaaggaatgttca *F agaaattttataatttgctgcctatcacgtaacttcccgttgtttatttacactatgaatatgaattctactatctg *F ccccctgctggctgatggcctggcgacgcccttgacaaaatatatgtaaaaataatattacaaaatgttacaacaaa *F gtttgattgaagtttatttgtcttggttatctatcagtacagcaaaacattttagccgcgccccttccaaagcccac *F aagtcgctcaaaactgtcatgtcaacacgtttcaatatattattttctggccatatggaatctgatagtcaaggaac *F tcgactatagcattctctctttttttattcttatgttggtcatacgttatcaagttacacaa *F It turns out that this is a large 1200aa protein in others, and we have *F just the C-terminus. Indeed we need to add it to CG2165, providing the *F C-terminus for this gene *F \*************A. mellifera Contig253 TGTCCGTACTCGTTGAAACGCATAAATACACGCTAATGAA *F TAATTTTTACTGATGTATATAGGCACTTTTTTCTTCGTTCTCCTCTTTCTCTCTCTCTCTCTCCTCATTCTTTCCGT *F TTCTCACTTTCTCTCTCTCTCTCTCTCTCGTTTCATTCTTCTTACGCTCTCTCTTTCTCTCGCTCATATACTATTCC *F AAAACAACAAATTTGTTTATCAACCTTAAAAGGCTCCTTTTTTTTTTCCCGATAAGAAAAGCTTGCAGCTTCAAAAA *F CAGATATTTTTTTGTGTTTAGACGATCGTTTTCTTAAAAGCTAAAAAACATTATGAAATCGAATCAAAAATTCACGC *F CTATCATCATCTCAACGAAGAATCGGTCAAAAATCATCTTCGTTAACGGAAAGTCTTTTGATATATATAAAAAGGTA *F TCGAGACAGTGACAGAAGGAATTGAAAGCGGGTTGAAGGAAACATTATGAGAACAATTACGTGCCTCTTAACCTCTA *F CCAAGTCTTTTACATAGTCGCTTTGATATGGTAATAAATAGGAAATATTTCACGTTTCACGAGAGGATGTAATACAA *F TAATACAGTTTAGTTGGTTAGAAGAGAATAGAGAGCTGTATGAAAACAGAAGGTAGAGGTAGAAATAGGAAAAAGAT *F ATAACGACAGATAGAAGAATCTCGAGCGAGAGGCTGCTGTTACATTTTCGCATTTTCGTTCTGTTTCCGGACGTAGT *F AATTCGTACTTAC *F long ORF in RC encodes 17% arginine and 14% glutamic acid; 230aa *F protein; BLASTX match to eukaryotic translation initiation factor 4B *F Homo sapiens 40% e-08 and in yeast; genomic match is full-length and *F clear but low at e-09 to C-termianl annotated region of CG10837, so *F think have additional C-terminal region of this gene; one EST *F \*************A. mellifera Contig2709 TGAAAATGCGAAAAATACAATAAATGGTGGAAAATATAG *F CAATTTAAATATACCAGTAACATCACAACAAGGATTAGCGCCACTTAGTCCCTATTTAAATTTTGATCCTGCATATC *F TTCCTCCAAGCCAACCAGAATATATATTTCCGGAAGGAGCAGCAAAACAAAGAGGAAGATTTGAATTGGCTTTTAGT *F CAGATTGGTGCAGCATGTATTATAGGAGCTGGTATTGGAGGTGCTACTGGTTTGTATAGAGGCATTAAAGCAACATC *F TTTAGCTGACCAAACTGGGAAACTTAGAAGAACACAATTAATCAATCATGTTATGAAAAGTGGATCGTCGTTAGCAA *F ATACATTTGGAATAGTATCTGTGATGTATAGTGGATTTGGTGTGCTTTTATCTTGGGTCAGAGGTACAGATGATTCC *F TTAAATACATTAGCAGCAGCAACTGGAACAGGAATGTTGTTCAAATCTACAACTGGCTTAAAAAAATGTGCATTGGG *F TGGTTGTATAGGACTAGGAATAGCATCTGTATATTGCTTATGGACTAATCGAGAAGCCTTACTGGAATTGAGGCATC *F GCAATATAAATCCAGCGTAAGACTGTGTGTAACAGCAAAAACTCTGAATATTCCTAAATATTTTTCTTATAGTGATT *F TAAATTTCTTAGTAGTACTAACAAAGGAATGATAGAAGGGTTTGCATTCTGTAATTGATATAAATTATATATGAATG *F ATTATTTTTACAAAAATAAAAAAAAA *F long ORF encodes 13% glycine >200aa protein; BLASTX matches full-length *F at 43% and e-31 for 200aa translocase of inner mitochondrial membrane *F [Mus; *F genomic match is to a small 15kb contig; several ESTs, so annotation *F would be easy \- NEW GENE *F AE003403.2 TTGAACACAGATGTCACTTCTACAGGGGAAAAAAGTTTAAAAACA *F AGTAAATCACAGAAAACGTCGTTTCCTTTTGCTAATAGAGCGCCTGAATTCGGTGGAAATAGCAAAAATAATATATC *F ACCATTCTTGGGACTGCAAACAAATTCGAAAATGAGTGACAATTTTTCAAGAACACCATATTCTGATGGGCACGCTG *F CAACCCgtaagcaacaaagaatttggttacataattttattaattattaaatagattgctgttatcttttttctttg *F taaattgaaatgcaatacaatatgcagATGAGGAAGCATCAAAACCCCACTACACTACCACTACGAGTTCTTTTAGT *F AGAACTCCGGTCTCGCCGTACCTCAACTATGATTCGCGATATCTGCAGCAAGCACAGCCAGAGTTCATTTTTCCCGA *F AGGGGCCAACAAGCAGCGTGGACGCTTCGAGTTGGCCTTCTCTCAGATAGGCACTTCGGTAATGATTGGCGGTGGAA *F TTGGCGGCCTAGCAGGTGTTTATAATGGTTTAAAAGTCACAAAAGCACTCGAGCAGAAGGGAAAAGTTCGTCGAACA *F CAgtaagcaattggcggaattaaaattggttgcaacctcacaactttgactcaacacgtagGTTACTTAATCACATT *F ATGAAGCAAGGTTCCGGCACAGCTAACACATTAGGTACATTGACGGTGCTGTATTCGGCTTGTGGAGTTTTGCTGCA *F GTTTTTCCGCGGAGAAGATGATCATATAAACACAGTAATTGCGGGCTCTGCCACAGGACTATTATACAAGTCAACAG *F gttagctaaattttccatatatcgaaaaataatatttattaactagttgcattgtattttacagCTGGCCTTAGGAC *F GTGTGCTTTTGGTGGAGCTATTGGGCTGGGCATCTCGTCCCTCTATTGCTTATACCTAATAGCACAGGAAAACAGTT *F CGAACTCAAGTCCCAAATACCTATAGATGGCTGAAATATGTAGTACGCAGGCATTAATAGGATCACTCCTAGCCGAT *F TAAAATTATAAATACGAAGTTTTAATTTTATTTTGTTTTATTGCATTTTATACTAAGCATTTTTGCATTAACTTGCT *F GTTGTAGATAAAGCCATCACATTCCCCCACGCAATTTAGTTAGGAACCCAATTTCCAAACTCGCTAATAGTCCAAGT *F TTTTGGTATCGGCGCCTACCATGATTGCCCTGCTGCCCCCAGCCATTTCATTATTGGCCGGAGCTTCGCAGCTGGTA *F TCCTGTGTGGAGCACGTCTGGCAGTGACAGTTCACTGCCTCCATGTACTGGTACTTGCTTACGCTGTCCTCTGCTTT *F AGGGTGACAATTCTTTAGGATAGCTACTACCAGCTGCCGCTGGGCGTGGACACAAACAGGATGGAACGAACGCTTGT *F AAGGAAACTTCCAATCGGAAATTTCGCTTGAGTCGCATCGTCCCCAACACGACCAAACGCTTACATAGTCCCAACAC *F TCGTGTCCTTGAAGATCGGACTGTGTCACTTTATACGTGTATACACGACGATGGCATCCCAAAGGCGTCACGATATG *F TCCGTTGTTCATCGGCTTAATTTCCGACAAACTTGAAGATGAAACCGAAACAAGCACAACAGACGTACCTACAAAGA *F TAGCTAAAGTCCTGAAAAAAATTATTCTGAGCATAGATGAACAATGC *F LP07554.5prime *F TTGAACACAGATGTCACTTCTACAGGGGAAAAAAGTTTAAAAACAAGTAAATCACAGAAAACGTCGTTTCCTTTTGC *F TAATAGAGCGCCTGAATTCGGTGGAAATAGCAAAAATAATATATCACCATTCTTGGGACTGCAAACAAATTCGAAAA *F TGAGTGACAATTTTTCAAGAACACCATATTCTGATGGGCACGCTGCAACCC-------------------------- *F \------------------------------------------------------------------------ATGAG *F GAAGCATCAAAACCCCACTACACTACCACTACGAGTTCTTTTAGTAGAACTCCGGTCTCGCCGTACCTCAACTATGA *F TTCGCGATATCTGCAGCAAGCACAGCCAGAGTTCATTTTTCCCGAAGGGGCCAACAAGCAGCGTGGACGCTTCGAGT *F TGGCCTTCTCTCATATAGGCACTTCGGTAATGATTGGCGGTGGAATTGGCGGCCTAGCAGGTGTTTATAATGGTTTA *F AAAGTCACAAAAGCACTCGAGCAGAAGGGAAAAGTTCGTCGAACACA------------------------------ *F 2----------------------------GTTACTTAATCACATTATGAAGCAAGGTTCCGGCACAGCTAACACATT *F AGGTACATTGACGGTGCTGTATTCGGCTTGTGGAGTTTTGCTGCAGTTTTTCCGCGGAGAAGATGATCATATAAACA *F CAGTAATTGCGGGCTCTGCCACAGGACTATTATACAAGTCAACAG-------------------------------- *F 1-------------------------------CT *F AT20116.5prime *F GGCACGAGGCTTCTACAGGGGAAAAAAGTTTAAAAACAAGTAAATCACAGAAAACGTCGTTTCCTTTTGCTAATAGA *F GCGCCTGAATTCGGTGGAAATAGCAAAAATAATATATCACCATTCTTGGGACTGCAAACAAATTCGAAAATGAGTGA *F CAATTTTTCAAGAACACCATATTCTGATGGGCACGCTGCAACCC--------------------------------- *F \-----------------------------------------------------------------ATGAGGAAGCAT *F CAAAACCCCACTACACTACCACTACGAGTTCTTTTAGTAGAACTCCGGTCTCGCCGTACCTCAACTATGATTCGCGA *F TATCTGCAGCAAGCACAGCCAGAGTTCATTTTTCCCGAAGGGGCCAACAAGCAGCGTGGACGCTTCGAGTTGGCCTT *F CTCTCAGATAGGCACTTCGGTAATGATTGGCGGTGGAATTGGCGGCCTAGCAGGTGTTTATAATGGTTTAAAAGTCA *F CAAAAGCACTCGAGCAGAAGGGAAAAGTTCGTCGAACACA------------------------------2------ *F \----------------------GTTACTTAATCACATTATGAAGCAAGGTTCCGGCACAGCTAACACATTAGGTACA *F TTGACGGTGCTGTATTCGGCTTGTGGAGTTTTGCTGCAGTTTTTCCGCGGAGAAGATGATCATATAAACACAGTAAT *F TGCGGGCTCTGCCACAGGACTATTATAACAGTCAACAG--------------------------------1------ *F \-------------------------CTGGCCTTAGGACGTGTGCTTTTGGTGGAGCTATTGGGCTGGGCCATTTGTC *F CCTCTATTGC *F GH02609.5prime *F CAGGAATGCTTGAACACAGATGTCACTTCTACAGGGGAAAAAAGTTTAAAAACAAGTAAATCACAGAAAACGTCGTT *F TCCTTTTGCTAATAGAGCGCCTGAATTCGGTGGAAATAGCAAAAATAATATATCACCATTCTTGGGACTGCAAACAA *F ATTCGAAAATGAGTGACAATTTTTCAAGAACACCATATTCTGATGGGCACGCTGCAACCC----------------- *F \----------------------------------------------------------------------------- *F \----ATGAGGAAGCATCAAAACCCCACTACACTACCACTACGAGTTCTTTTAGTAGAACTCCGGTCTCGCCGTACCT *F CAACTATGATTCGCGATATCTGCAGCAAGCACAGCCAGAGTTCATTTTTCCCGAAGGGGCCAACAAGCAGCGTGGAC *F GCTTCGAGTTGGCCTTCTCTCAGATAGGCACTTCGGTAATGATTGGCGGTGGAATTGGCGGCCTAGCAGGTGTTTAT *F AATGGTTTAAAAGTCACAAAAGCACTCGAGCAGAAGGGAAAAGTTCGTCGAACACA--------------------- *F \---------2----------------------------GTTACTTAATCACATTATGAAGCAAGGTTCCGGCACAGC *F TAACACATTAGGTACATTGACGGTGCTGAATTCG *F translation *F M S D N F S R T P Y S D G H A A T \-------------------------- *F \--------------------------1---------------------------------------------H E *F E A S K P H Y T T T T S S F S R T P V S P Y L N Y D *F S R Y L Q Q A Q P E F I F P E G A N K Q R G R F E *F L A F S Q I G T S V M I G G G I G G L A G V Y N G L *F K V T K A L E Q K G K V R R T Q------------------------------ *F 2---------------------------- L L N H I M K Q G S G T A N T L *F G T L T V L Y S A C G V L L Q F F R G E D D H I N *F T V I A G S A T G L L Y K S T \-------------------------------- *F 1-------------------------------A G L R T C A F G G A I G L G *F I S S L Y C L Y L I A Q E N S S N S S P K Y L Z *F several intron splices not predicted, but cDNAs make it clear, as well *F as matches. Interesting 5' UTR, has no stop codons, but this is the *F first M and it aligns okay. *F \***********A. mellifera Contig764 GATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA *F TGCATGTCTCAGTACATGCCGAATTAAGGTGAAACCGCGAATGGCTCATTAAATCAGTTATGGTTCATTAGATCGTG *F GACACATTTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAACAGAATTCCTCTCAGAGATGGGAGGAA *F TGCTTTTATTAGATCAAAACCAATCGGTGGCGGACGGCTCGTCCGTTCGTCCATCGTTTGTTTTGGTGACTCTGAAT *F AACTTTGTGCTGATCGCATGGTCATCTAGCACCGGCGACGCATCTTTCAAATGTCTGCCTTATCAACTGTCGATGGT *F AGGTTCTGCGCCTACCATGGTTGTAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACAG *F CTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGGCACGGGGAGGTAGTGACGAAAAATAACGA *F TACGGGACTCATCCGAGGCCCCGTAATCGGAATGAGTACACTTTAAATCCTTTAA *F no obvious ORFs; WEIRD BLASTX match for RC full of stop codons, at 70% *F and many gaps and e-14 to human non-functional folate binding protein *F Homo sapiens; Needs four stop codons included! *F Is this a mouse pseudogene cDNA? No, has genomic match in Drosophila *F at e-37, but to a tiny incomplete 1000bp contig \- so will not be able *F to reconstruct this amazing gene; AND tons of 70% B. mori and some *F Drosophila ESTs with their own set of stop codons in them! What on *F earth is this gene? Mitochondrial code doesn't fix problem *F Contig 764 RC TTAAAGGATTTAAAGTGTACTCATTCCGATTACGGGGCCTCGGATGAGTCCCGT *F ATCGTTATTTTTCGTCACTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTA *F GCTGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTACAACCATGGTAGGCGCAGAACC *F TACCATCGACAGTTGATAAGGCAGACATTTGAAAGATGCGTCGCCGGTGCTAGATGACCATGCGATCAGCACAAAGT *F TATTCAGAGTCACCAAAACAAACGATGGACGAACGGACGAGCCGTCCGCCACCGATTGGTTTTGATCTAATAAAAGC *F ATTCCTCCCATCTCTGAGAGGAATTCTGTTTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAAATGTGT *F CCACGATCTAATGAACCATAACTGATTTAATGAGCCATTCGCGGTTTCACCTTAATTCGGCATGTACTGAGACATGC *F ATGGCTTAATCTTTGAGACAAGCATATGACTACTGGCAGGATC *F translation *F L K D L K C T H S D Y G A S D E S R I V I \# F V T T S *F P C R E W V I C A P A A F L G C G S C F S G S L S *F G I E P \* F P V T R Y N H G R R R T Y H R Q L I R Q *F T F E R C V A G A R \* P C D Q H K V I Q S H Q N K R *F W T N G R A V R H R L V L I \* \* K H S S H L \* E E *F F C L H V L A L E L P Q L S K \* *F AE003241.2 entire AGTGATCCACCGCTTAGAGTTTTATAATTCATTTTTATATAATGTCAATTATGT *F TTTTATTGAAAGAAATTAAAAATACACCATTTTACTGGCATATATCAATTCCTTCAATAAATGTATTTATATACCTA *F AAATAAATGTTGCGAAATGTCTTAGTTTCATATAAGCATTATGTATCATAATAATCTGGTTGGTTATGGGGTTTGCT *F ATTTTGGGTGACACATACTGCAATTTATATAAAACATTAACCTGATGGATGCCAGGTACAACATTGTTTATTTCAGG *F TTGTTGCATTAGCCAACGTATGCCCATAACTAAGATGAACAATACATATTCGCAACGCGTGTATAGTAATAAATACA *F CACAAATTTTAAAAATTAGTTAATATCTACCAATTATATTAACACTTATTTCGATGATTACCACACATTCGAAATTA *F TTTTATTTTGATTCGACTTCCACTTTCGAATTTTGTTTTTTCGATTTTCATGTTCGAAACATTATTTTTATAGGAAA *F CGCCGTTGTTGTAAGTACTCGCCACAAATACGCACAACATACATTAGAAATGTTAAAATCTTTTTATGAGGTTGCCA *F AGCCCCATCTTCGTTTTATTTTGATTTTAACTTTTTGTATGAAAAGATACAAGTATTTAATCACATATAAGAACTCC *F ACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG *F TTCAACTACGAACGTTTTAACCGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCA *F GACTTGCCCTCCAATTGGTCCTTGTTAAAGGATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCC *F TGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTG *F GTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTCCTAGA *F TACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGGCGTCGGTACAAGACCATACGATCTGCATGTTAT *F CTAG *F translation *F L K D L K C T H S N Y R A S D M S P V L L F *F F V T T S P S W E W V I Y A P A A F L R C G S R F S *F G S L S G I E P \* F P V T R C N H G S P R Y Y H Q *F K L I G Q T F E R S G V G T R P Y D L H V I \* *F Yikes, all the cDNA matches are backwards too, that is to the RC as *F with the honey bee, and even for the vertebrate clones? What on *F earth can this be? *F \************A. mellifera BB260003A10H2.F ACAGAGAGAAAAAGCAAGCTGCCTTACCTATCGCT *F GTCTTTGGAATGGAAATGGTGGAGAAATTCTATTCGAAACAATTCACGGATAAGGAAGAGGGTCTGATGCAATTGAA *F AGAAGAATTAAAGACGTTTGATCCAGAAGTTTCGAAACATTCCGCGAATAAAACGGCCAGAGCTGCGATTTTGTTGT *F TACACAGAGCCCTTAGGGACAAAGTTTTCAGTGTATACAGTCTAGCTGCACAATTGATTAGAATTTTTTTTTCAGAA *F TTTGCAACTAGGGTATCTTCTACGGAGATTGCGAGAAGTGTGGAAAGATTGCTCCCAGAATTATTGACTAAATCAGG *F GGATACCACTCCAAGGATTCATAACATGGCAGTCCACACGATACTCAGTATGGCAGATTGTAAATGTGTCCGAGAAT *F TGCACATTATACCAGTGCACTTAACAAGACCTGTCAGCAGTAGCACTCATCAAAGATTGGCGTTGAGTAGGTTAGAA *F ATGGTGGAGCAGTTGATTCTGAGCCATGGAATATCTACTGATAAACAAAGTGGGCTCACTTGTCGAACATTGTCGGA *F ATTGGGTTCTACGGGATTGCATCATCCGGCGGAAGCAGTGAGAAAAGTTTCGGAAAGAATTCTAGTGTTGGTGTACA *F AAGTGAATCCTAGATTGGTTCGCAAACAATTGCCTCCTGACGATGATATTACCAGGAGAAATTTATTGTATCGTCAA *F CTTTTTCACGAATTTGATGTTT *F full ORF; BLASTX glycine-, glutamate-, *F thienylcyclohexylpiperidine-binding protein [Rattus; 28%; 3-22; could *F be rest of CG10137; no ESTs] *F Indeed, there is a human protein, KIAA0562 , that is about 900aa long, *F and its first part matches CG10137, while its second matches our cDNA. *F Similar long proteins are known from C. elegans and Leishmania major, *F so the truncated rat protein above of 400aa, is aberrant. *F \*************A. mellifera BB260003A20B1.F CCTTAGATGGACCGATCTGATATGCAACCTAATA *F CAAATCAATTTACTGTATGGAGTCCTGCAGGCCAAGACATTAGTACGAGGGTCTCCTGGAATTCTGATCGACGACCA *F ACCGCCCGAGAGCCCGAGCGACAGTAACGAAACCGACGAGACGAATAAAAATTTCCCGTGGATGAGGGTGTTGGCCC *F AGTTCGCCAACTCGTTCAACTTTTACTGCTCCCATCAGAATTTCTGCCACCCGTATTGCCACAGGCGGCAGATGCGC *F GCGTGCAGCAGATTGATCAAGTCCATAAGGAAAATCTACGGGGAGGAGTTTGGCATATTGAACGGGACAGGGATATT *F CGACTTGGACACGGATAAGAAGGAGGCGAGCAAAAAGGAGAAACGGAGCCGAAAAGTTTCGGAGCAGGCCAGCACCC *F AAGTGTCCCCTGTGAGGAGGAAGGACAGCGTAGGGAAGAAGTACAAGTAACATTTATCTTCCTTAATTGATTCAGTG *F AAAACTGACTGTTCTAATAATTTCCTGAATAAATTTAAGATAAGATATTCTTCAAACACAATTTGGATTAACATCAA *F CATCTCTTCAAATTATTCTAAGATTATTTCTATATAATATATTTCTTATTATAAGATCTTATCTCTAAGATCTGTTA *F AAGAAATGCATGGCTCTATTTTATTAATATTTTAAATACAATCAAACTGATAACTGGTCTTGAAAANTTATTCAAAT *F GAAAAGTGGCATCTTGCGTTCCTTTCCTCTTCTTCGACAGGGTTGAAAAGAACATGGATGGGTCTTAATTAGGCAGG *F CTCGCTCAACGAGACTTGTC *F end of ORF encodes weak match to C. elegans and human proteins; *F methyl-CpG binding protein 1 [Homo; 32% over 84; e-0.27; unannotated *F NEW GENE; no ESTs *F The human protein is about 600aa long, with our matching in the middle; *F this region has two zing fingers, but the methyl-CpG binding domain is *F at the extreme N-terminus; *F This genomic match is very good at about 55% so see how far it can be *F extended, we have 14kb region to play with! *F AE003762.2 CACCTCGGAACAGGGCATGATCAGTGGTGGCGAGGAATCACCAGGAATTCTCAG *F TGACGATCAGCAGCCGGAGTCACCAACGGACTCGAATGAAAACGATGATACGGCCAAGAATATGCCGTGGCTAAAGG *F CAATTATCGATCTTATGTCCAGCTATAACTACTACTGCACCCATAAAGGATATTGCCATCCATTTTGCTATAAACGG *F CACATGCGATCCTGCACTCGCTTGGTCAAAGCCACCAGAAAGgtaagataccatttacggaagtcaaacttaagcca *F aaaaattcgattgtttcctagGTTTATGGCGAGGAGTTTGGATTCACCTTCGATGCAGACCATCCGAATGTGGAGCC *F CACTATCATCACCTCCAGTAAGCCACATACTTCTCGAGCTCGCTCCACTAGAAAAGTATCGGAGCAGAGTTCCACTC *F AGACATCTCCGTCCAAGCGAAAGGATAGCTTGTCACGCAAAGATCGGTGGGTAGTATTCATAGTTAGCCAAAACATA *F GCTTATATATACATGCTCACAGGA *F translation *F S P G I L S D D Q Q P E S P T D S N E N D D T A K N *F M P W L K A I I D L M S S Y N Y Y C T H K G Y C H P *F F C Y K R H M R S C T R L V K A T R K \------------------- *F \---0--------------------------------- V Y G E E F G F T F D A D *F H P N V E P T I I T S S K P H T S R A R S T R K V S *F E Q S S T Q T S P S K R K D S L *F Now figure out that is already recognized as CG18437, except for some *F reason the entire annotated mRNA is not translated into the protein *F CG18437? *F \**************A. mellifera BB260003B20H2.F GAAGATATAAAAATTATTTAACGAAAATCTAAA *F TTTTTGTCGAACGATTAAAATTTTGCGGAGTTAACCTATACGTTTACATTCAATTACCAATAACGACACATTATCAT *F AAAAGAATTTCCAACATTAATATCACGGAAAGATTAACGAGGTGGGGCAGAATTACTTTTCAATAAAAAAAAAAAGG *F CTCGAAGTAAACTTACCTGGAGTAAACGAATGGACTATACAAAAGCTACTTTTCTGGATAACAGATAATCTGTTAAA *F GGAACGACAGGAACTTTTTATACAAGGGGATACCGTGCGTCCAGGAATCTTGGTTCTTATAAATGACATCGATTGGG *F AATTATTGGGCGAAAGCGATTATAAAATAAAATCAGGCGATACTATATTATTCATATCCACTTTACACGGAGGATAA *F GAAAGATAAAAGTGGAAAAAGAAAAGATAAAAGTCGATGCAAAGAAGATCGAAGATAATACCGCATAACATAATATA *F AGTGGAATCCGAGAAAAACGTAATTCTTCAGAAAAACATAATTTCACGGAATGTTTGTACCGATGGTGAAATACGTG *F GATACGCGCGTAGCGTTAAAAAAAAAAAAAAAATAAACCGTTTATTCCTTCGTAAGATAAGTAACGATTTCTATTAG *F CCCCGCCGG *F no clear ORF, yet ubiquitin like protein; Urm1p [Saccharomyces *F cerevisiae]; 47% 3-11; has no start codon and a frameshift, but encodes *F full-length 99aa protein; *F TBLASTX is clear; unannotated in genome NEW GENE; no ESTs at all *F Only two kb available for this gene between annotated neighbors; this *F is the entire region *F AE003558.2 gtgttttttttaatttcataaattcacaacgaaatgtaaaatgtcttcttagag *F cacagatcataatatgctaatgaaaactttacctagcgttccattggaatgccacctgtttatgtttctcaatggga *F ataattataatgctgagtcccctcgtcgaaggtgaatggtaaaggtaaatttacgtacaaattataatttctttcaa *F aatgcaataatttttggaagtttagagcatgtggttgccagactttttagaaattaggattctatatttggtattat *F ttttgaATGGTGGATTTTTTTGAAAAGCTTAGACGCGGTCACACATTTATTTACATCGAACATATGATGGGCACGCC *F GGAATTAAAAATCATATTAGAATTCAGgtatataaagccttgttcgaattattatttccaataaatgagacaatttg *F taatttaatttcgtagTGCAGGGGCGGAGTTACTATTTGGTAACATAAAACGCCGTGAATTGAACTTGGACGGTAAA *F CAAAAATgtatgttctaaaagatgttttaaacttgagtgaaatcggttatgaattatatatattttaaagGGACTAT *F TGCTAATCTGCTTAAGTGGATGCATGCGAATATTTTAACGGAGCGTCCGGAACTTTTTCTTCAAGGAGATACTGTgt *F aagtttggcttaaactataagggatacatagtctatactttctattgtatgttttcagGCGACCTGGAATTTTAGTA *F CTCATAAATGATACAGACTGGGAATTGCTGgtaagtaagagtacagaatatggaattctaaaaactataattaactc *F aacttctagGGTGAACTGGACTACGAGCTGCAGCCCAACGACAATGTGTTGTTTATATCAACTTTACACGGTGGTTA *F AAAAACGTTCTGGAATCTAAATTATAGGAGAAAAGTTTATTTTTATACTACAACCCATTaaattgtattcaaaaaac *F aaacaaaaacagtttttgactgaatcagaattacgtccctttaccagaggcaaaggcccacacgatggttcgggtag *F taaagttttcatcatatcatctagagattgtaagctagcaggttttcgctatttataaaagcacagcattgaacaag *F cacttgggaattgtagggaagttaaaaatagaacaatccagatgcggtttggcgggtaattcgaagtaaggacaaca *F cgttagttttacattgaagcaacatttattgaatttaaattttcgcttaaaattatttatcgagttattatgaagta *F tagatatatttttttaatgttcgtttacgattattttaactataggcacttaggttacatatcaactaactgtacgt *F aatgaagttcgattcagatatgttgggcatcggccacgcccctttttcgggtgctgctcatggctcccatcgatttc *F tgtgtgtgtgtggtgcggatcgttcgattgtgtggactaagaactagatatgtatgaaacttgcttcgacattgaca *F cgctgaattcaataatttcgactgatttttccgataaacaaggcaaaacgaaaagcagcgactatgacaaattaacg *F aaaactaaaaatgtaataaataataaataacaaaaataatgaaataaaattagcgatcgaacgtaatacgatgatac *F tacatgggatccgatgaaaccgttctgctaaagctattaatggggatttatactatatctagatgcgat *F translation *F M V D F F E K L R R G H T F I Y I E H M M G T P E L *F K I I L E F S--------------------------------2------------------------ *F \--------- A G A E L L F G N I K R R E L N L D G K Q K *F \---------------------------------1-----------------------------W T I A N *F L L K W M H A N I L T E R P E L F L Q G D T V--------- *F \-------------------------2------------------------- R P G I L V L I *F N D T D W E L L \------------------------------0----------------------- *F \-- G E L D Y E L Q P N D N V L F I S T L H G G \* *F Lovely small compact gene with all intron boundaries predicted; encoded *F protein is 121aa *F Drosophila *F MVDFFEKLRRGHTFIYIEHMMGTPELKIILEFSAGAELLFGNIKRRELNLDGKQKWTIANLLKWMHANILTERPELF *F LQGDTVRPGILVLINDTDWELLGELDYELQPNDNVLFISTLHGG *F Honey bee *F RGGAELLFNKKKRLEVNLPGVNEWTIQKLLFWITDNLLKERQELFIQGDTVRPGILVLINDIDWELLGESDYKIKSG *F DTILFISTLHGG *F \**************A. mellifera BB260004B10A11.F GTTGCGCACTCCTAGAAACGCATCGGCAACGC *F GAAACAACTGGCATCAAGAAATTGTACAATAGCTTCTTCGTAATGTTCTAAGGATAACTCTTCCTTAAGGAAGTTCG *F GCCCTTTATTAACCGGTGGTCGAGTGATGCACCGTGCACCTCCGAGATCATTGTCGATCCCGATCGAGGAAAATACG *F CGGAGAAAACGCACAAAAGAAGGAGGACGTTTTGGTTAGTAAACGAAAGAAAAGGAACGAGCTGAGCGGAGGAAGCC *F GAGAAGCGGCGAAAACAAAGAGGAAAAAAAAAAAAAAAAAAAGCCAC *F tiny match 88% over 25aa at e-07. Could be end of an ORF. No BLASTX *F matches though, so novel protein; one Drosophila EST too. *F Match is to an unannotated region of about 5kb, so try to figure it out. *F AE003830.2 cgtaaacaactactatataatgtccgtcgccatctgacagtggtccaaacaacc *F agcacaaagagctagaagtcgccgcagccagtgacaagtttcaccacagcgagtgagacctgtaccgtagaaccaac *F acaagacacttaagcttgcaacacgggctaaccaattcagcgataaATGGAGAAATCTGAAATACGACTGCAACGCA *F TGTCTAATGAATATCAGTCGCAATCGAGCTATATGTACCTCCGGACCAAGATGCTGTTAAAAATCGAGAATACCCTA *F CTTCGAAGCCATCGTCAGCGCGAGACCACCGGTATCAAGAAACTATACAATTCGTTTTTCGTATTGTTTTAATTTGC *F CCCCCCGGCCAGT *F translation *F M E K S E I R L Q R M S N E Y Q S Q S S Y M Y L R T *F K M L L K I E N T L L R S H R Q R E T T G I K K L Y *F N S F F V L F \* *F This is all I can annotate, a simple little ORF, the second half of *F which matches our honey bee EST as well as one Drosophila EST (which *F has other problems including a piece in RC. *F No idea if it is real, no BLASTP matches. *F \*************A. mellifera BB260009A20B5.F TTTTTTTTTGAATTCTGTCCGAGATTCCATTCTT *F AAAAAAGAAAACAAAAGCTAAAAGAATTAATAAGAAAAAAAATATATATATACACATATATTAAATAGTATAAATAA *F ACATAACCTATAAATAGTAAAATATTCACATACATTTTAAAAGATTATTACTATTCTTAATAATAGACTTATAATGG *F TTCTTCGCTATATCAATAAATTCTTATATTATTTAAATAAAATTATAGATTGAAAAAAATGATTTAAGTGAAATTAA *F ATATCGAAAAAGAAAATAGAGATATCCACTTCCATACACAATATAAAGGGAAATGTAAATCGAAGTTAATTGTGATA *F CAAATGCATACAAGAGAAATTAAGAAATACAATCTTATTTCATTATCATGCTATTTAAAAATACATTATATGAAAGC *F AACTTTATTATGAAGTATCAAGAACTCCATTTTTATTCGTATTATATCCGGGACACATGGTTATTTTCTAACCTTAA *F TAATACGTTTTAGTTTGGGTAGTGATTATTTAGAAATATTGAATGGAATATAAGATAAAAAAATCATGTCAAAGCTT *F TTTGTTTTATTTGAACATGCTGCTGGCTATGCCATATTTTCTGTCAGAGAATTTGAAGAAGTGGGAATGTTATTGCC *F TCAAGTTGAAGCATCTGTAACAGATTTGTCTCGTTTTAACTCAAGTGTGAAATTAATNTGGATTTTCACCTTTTAAA *F ACTGGCTTAACAGCTCTAGAAAGTATAAATAATATTTCTGAAGGAATTGCCCACA *F no obvious ORFs; frameshifted ORF at end of sequence (long 5' UTR or *F chimeric?) has excellent match to nucleolar protein [Drosophila *F subobscura]; tons of ESTs; why is it not annotated? *F CG13849 is annotated for the same region, but the amino acids are *F different? No, is part of this gene CG13849. *F \*************A. mellifera BB260010A20C3.F GAAAAGGAAAACTCCATCAAAATTAAAGACCATG *F GGTAATCATGATGACTTTTTAAATCGTATATCTAAATCGCTTTATTATGCAAAACTGCCAGTGACTGATTGCCTCAG *F TTTACCTGTTACTGAATTGGCAGCAGAATTATTCACTGAAGTGAAGAGTGGTTATACACTTGAAAGATTAGATGTAG *F AAGAGGCTAGTAGAATTTCTAGAAATGCATGTGTATCACCATGTTCTCTTGTTTTGGCATTGTTATATTTGGAGAGA *F TTAAAAGATTGTAATCCAGAATATCTTCAACAAGTGGCACCTTCTGAGCTCTTCCTTGTTTCTTTGATGGTGGCTAG *F TAAATTTTTAAACGATGAGGGAGAAGATGATGAAGTTTTCAATACTGAATGGGCACAATCAGCTGATTTGACTATAT *F TACAAATAAATCGGTTAGAAAAAGATTTTCTTAAAGCTATTGATTGGACTGTTTTTGTTCATAATCAAGATTTTTGG *F GAAAGATTGCAGAAATTAGAAAGAGATATAGCTTATAAGGAAGCACAAAAAAGAGGCTGGTTTTCATATACAGAATT *F AAGTTGTCTAATGAATTCAATGCAATTAATTGCAGTAGCACATGCTGTAGTAAATGTATCATCTATTTGCTTAGCAA *F CATATACTGCANGAGTAGTTACTCTTTTAGTTCTGCTTTAGTTGCAAGCTATCTTCCAGGAACAGTACTTAACAATC *F CAAGACAAGTAACTAATTCTACAGATATTATGAAAGCAGATTTTAAATTCAAGATGGATATAACATCACCTATCGAA *F ATTTATCAGAAATGTTTTACAACAGATTTATATC *F long ORF encodes BLASTX match to CGI-57 protein Homo sapiens; e-37; *F and nematode protein; these are 400aa proteins, and match is from the *F N-terminus, our ORF may be frameshifted at end *F TBLASTX match is to five regions in tiny unannotated scaffold; one *F Drosophila EST and a Bmori EST *F AE003132.1 TTCGCATATTTCAGTTATTTATTTAGAAATGGGGCGATTTAAGTTATGTGCTTC *F GCCGAGAGAGgtatttaaaaagttttcacgaatatatgtattagcaaattttttaaatttccagGTTATGAAGTACG *F AAGACTTTATAAAACGCATTCGAAAAAGCCTCTACTATGGCGTTGGAACACCAGACACAGAAATGTCGGTCTCCTTA *F CCCTTTGCGGAGTACGCGGCAGATTTGTTTTCGGAGACTCATCGCGGGCATTCTTTGCATCGCCTAAGTTGCGTATC *F TGCTGCACAAGTACATGCCACGCCTTGCTCTTTAATTATGGCATTGATATACCTCGATCGCTTAAACGTCATCGACT *F CGGGCTATAGCTGCAGAATCACACCACAGCAGCTGTTTGTTGTGTCACTAgtaagtacgcactcctctataacttgc *F aaactaatgcaaacaacaatatgaacgcaccgtaaaaaagtacatggctataaatgtcgaaactgtagctgctgaaa *F caaatttccgttagtttcactgtcggctgaatgaaaaatgacgatgattttgatcagaaataaattgtaaaatttca *F cagcggcactcactgtgtctgtacatgcactcagtcagcaagaagttttgacgtggctaccattgcttgagtccgct *F ttaatacatatgtgattgtctgtttttaatatggtaattataagttgaataaatggtaattatctttacagATGATT *F TCCACAAAATTCTACGCGGGCCACGACGAACGGTTCTATCTGGAAGACTGGGCCAGTGACGCTTGTATGACGGAAGA *F TAGGCTCAAGGCAGTCGAGCTCGAATTTCTTTCCGCTATGgtaaactttacaatgtctaaaatacaaaaataaaata *F cgttttttctagGGTTGGAATATATACATATCCAATGAGCTATTCTTTGATAAGTTAAGAAACGTTGAACGTTCTTT *F GGCTGAACAGCAGGGACTGCGTAGAGGTTGGCTCACTTACAGTGAGCTCGTGCAGTTGCTGCCTAGCCTTGAATGGA *F CGAAATTCCTCGTTAACAGCCTGTCTGTACTATCTCTAAGCTATGCGGCAAGTATTATAACATTAGCCGGAGCTTTT *F TTTATTGCGAGCCAAGTTCCCGGTACGTTATGGCATCGGGATGTGGAAACTGCCTCAGATTTCACCATGACAATTAG *F CAGTCAGGTATCCGTTTCAAATGCATTAGAGTCCACACCTTTTATTAATGTCCAAGTATCCTCACTTTTACGTAAAA *F CGAGTAACGTGAATGTTGAATTGATGAATCTTGAGAAGACAAGCTGCGCCAGGGCAAGACTGAATAAAATTGAATAT *F AAGCATCCGCGCCATCAATCAGTACCTACGCTTTCATTCATAAGCACCTGTCCACAACTTGATTTATTGTATGCCCA *F AGATGGAACAAGGAATTGGCTAAATATTAAATCGCCCAACAGCGACTACAAAAACAACAGAAACCTTTCAATAACAG *F TTAGATCCGTACAACTAGAAGAGCAAAAGGCTGAAAATGATTCCGTTATTTGGCAAGCCAACACCGAAGCAATGCAG *F TAAttgtttttaccgcaaaacttaaagaggtgctaacaactgatataaaataaatatatttatattatatataatat *F caatataataatattgataacaaattaaccaagcgtacgagtaatataacatgcataacagtaatacgaaactgctt *F ttatttcttcacagaactaatgttcgctggcttaatcaaactgtcataaaaactataatagcacattattatatgtg *F cctagaggtggatactttggatgctaaactaaaatgaacaaaataagttagattgttctatattatattaaaataaa *F atgtttctgttgctctacatataaggaaatacttttttaaggaacaaaattatggccatcggacttttagttttccc *F ggactttcgtccactcgaagcgtttttccgagataaataagttcgattattatacatacaagctgtaatatgttgcg *F ctatacttcaaagttactgccttacactgaccgaaatcatttacaaaacaagagagaattctataatcaagttcccc *F aactgtaactcagctggtgcaaagacactagaataacaagatgcgtaacggccatacattggtttg *F HL02313.5prime *F CATATTTCAGTTATTTATTTAGAAATGGGGCGATTTAAGTTATGTGCTTCGCCGAGAGAG----------------- *F \-------------------------------------GTTATGAAGTACGAAGACTTTATAAAACGCATTCGAAAAA *F GCCTCTACTATGGCGTTGGAACACCAGACACAGAAATGTCGGTCTCCTTACCCTTTGCGGAGTACGCGGCAGATTTG *F TTTTCGGAGACTCATCGCGGGCATTCTTTGCATCGCCTAAGTTGCGTATCTGCTGCACAAGTACATGCCACGCCTTG *F CTCTTTAATTATGGCATTGATATACCTCGATCGCTTAAACGTCATCGACTCGGGCTATAGCTGCAGAATCACACCAC *F AGCAGCTGTTTGTTGTGTCACTA------------------------------------------------------ *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \----------------------------------------------------------------------------- *F \--------------------------------------------ATGATTTCCACAAAATTCTACGCGGGCCACGAC *F GAACGGTTCTATCTGGAAGACTGGGCCAGTGACGCTTGTATGACGGAAGATAGGCTCAAGGCAGTCGAGCTCGAATT *F TCTTTCCGCTATG-------------------------------------------------GGTTGGAATATATAC *F ATATCCAATGAGCTATTCTTTGATAAGTTAAGAAACGTTGAACGTTCTTTGGCTGAACAGCAGGGACTGCGTAGAGG *F TTGGCTCACTTACAGTGAGCTCGTGCAGTTGCTGCCTAGCCTTGAATGGACGAAATTCCTCGTTAACAGCCTGTCTG *F TACTATCTCTAAGCTATGCGGCAAGTATTATAACATTAGCCGGAGCTTTTTTTATTGCGAGCCAAGTTCCCGGTACG *F TTATGGCATCGGGATGTGGAAACTGCCTCAGATTTCACCATGACAATTAGCAGTCAGG *F translation *F M G R F K L C A S P R E \-------------------------0---------------- *F \------------ V M K Y E D F I K R I R K S L Y Y G V G T P *F D T E M S V S L P F A E Y A A D L F S E T H R G H *F S L H R L S C V S A A Q V H A T P C S L I M A L I Y *F L D R L N V I D S G Y S C R I T P Q Q L F V V S L \-- *F \--------------0-----------------------------------0-------------------------- *F \---------0-----------------------------------0------------------------------- *F \----0-----------------------------------0-----------------------------------0 *F \-----------------------------------0-----------------------------------0----- *F \------------------- M I S T K F Y A G H D E R F Y L E D W *F A S D A C M T E D R L K A V E L E F L S A M \------------ *F \-----0------------------------------- G W N I Y I S N E L F F D *F K L R N V E R S L A E Q Q G L R R G W L T Y S E L V *F Q L L P S L E W T K F L V N S L S V L S L S Y A A S *F I I T L A G A F F I A S Q V P G T L W H R D V E T *F A S D F T M T I S S Q V S V S N A L E S T P F I N V *F Q V S S L L R K T S N V N V E L M N L E K T S C A R *F A R L N K I E Y K H P R H Q S V P T L S F I S T C *F P Q L D L L Y A Q D G T R N W L N I K S P N S D Y K *F N N R N L S I T V R S V Q L E E Q K A E N D S V I W *F Q A N T E A M Q Z *F Nice gene, with all intron termini predicted *F This is the available 5' region *F tacgcataaaaaaaaatttatccaaatatctccaatagtttaggaggtattaatttttgtaaaaaaacaggccaaat *F gccccatagtgcagcggcgtcaccatcgccgtggtcctaattgtttatgggatcatccaaaacatccgttactgtcg *F agtcaaaagacgacaccccgactccagcatcgagatgacgatatcttcccgaaaagccacggacgactttaacctaa *F cgcggggaggagttaacacgctaactccacgataagaccggacgcgggcgcagtaacgtcagcgcgactgcaactaa *F gtcgcgaaatatgactcctgcattccaacatgcccggagcgtgtgaagcgcaatgtcagtattctgccgtgagcgct *F gcttcagaagacgggctacttcatattaagcttaagttctctgtctttagtttaaaaactcatcagaacgcgcatag *F tcgcataataaatctcaataattaaaattgtttgttaatttatataaggctttttattcacgttgtttctctttcca *F gctcttgacttaagcttctcgacctcgataacactatcgcttgttcttaagacaagacaattaattctatcgatata *F agtgttagctagtattttatatttatacaccgtgtactcttagtattttatatttatagacagcttacaaaacaaaa *F aatcgaaaacttggggtttgaattaaacatttaatagtcaattatttctatttggcatatccctttttagtttttac *F tgcgcttggtaacgcctaatggtgtgcattaccatacaaaaattgtatgaactaaaaaagagtgtttctcttctcat *F cgtttctaaaaacctctgcatggtaatgccggcagcttgacgatttttttaaaagtaattaaaaatttattagatcg *F gtatcggttttttttataggactaaatagttttatt *F \**************A. mellifera BB260017A10H4.F AGCTATCATAGGTTAGGAACGATACATGTTTCA *F AAACCTAGTATACAAAGACGAAGCGGAAGTGATCCTAGCGAAGTAGCAAATGTTACAACTTTAGGCGAAGCTATTGA *F AAAGTTGAATACTTCAAAAGATTCGACTTTGAGAAGAAACTCTGGTGGTCACGCAACGGTAACACGAAGTCATAGTA *F GCTTATATGGATTAACGAGGCCAACCGATGAACGTTTAATAACACATCCGTTAAATCGTACATCTTCTCATGGTCAC *F TTAAGTTTCGAGGAAATGTGTAAAGGAAATGAAATAAAATCAAAAGTATGGAATTCAGAAGTTATAGCTCCACCTGA *F TGATGTTCAGACTCGACTAGGAATAGAGATGCTTACGCAACGAGATTTAACCAAATTACAACCATTATTATGGCTTG *F AACTTACTGCGGTATTTGACAAATATAACGTACCATTAAAAAAACGCAAACCAAATAAACGTCGAACAAAAGCTGGA *F AATCTATTCGGGGTTTCATTATCAACTCTTTTACTTCGAGATAGTCAATTATCGTCCGAGGAGAGTAATATTCCATT *F AGTATTTCAAAAACTTTTTAACGAATTAACGAGACGTGGTGTTAAAAAAAAGGGTATTCTTCGCGTTGGAGGACATA *F AGCAGAAAGTTGAATCAATATGCATGCAGCTGGAAACGGATTTCTATTGTAACCT *F full ORF encodes >240aa leucine and serine-rich oprotein; 31% over *F 144aa; 3-11 BLASTX match to KIAA1314 protein Homo sapiens; *F genomic match is to an unannotated region of a short messy scaffold; *F indicates that other missing genes might be similar; no ESTs *F AE003032.2 cctattgtcatttaataatactaattttatgcaaaatttaatttgtttactaaa *F aggaacaagggacagttacacgctcacgaagtgccactccggattccctggactctttacaaatcgATGAAGCTTGG *F ACCAACAATTCTTTGTATGTATTTTCCAATCGCTTAAGCCCCGTTTCCTTACTTTAAATTTATTCAATAGACCGACT *F TTTGTAAATGTATATGAAAAAAATACCGAAACTGCCATACAATGTGTAGAACAATCAAACGAAATATATTTAAAACA *F AAACCTGCGACGAACCCCTAGCGCCCCGCCTAAAAGCGGCACCTATGCGGATATATTTCGTGGTTCGCAAGTGCGAT *F GTGATATACCTTTGTACTCGGCGGATGGTGTAGAATTGCTTGGATATTCACGGATTGGCACCATACAATTTCCTCGG *F AACCGATCCGTCTCTGATCCATTTTGTTCTATTGGGTATGTTATTTTTAATAATTTGAGGACTCCTAAGGGGTTTGC *F CTTTCTAAAGTTATGTGTTTTCAGTCGATCAAAGGAGTCAAGAAGTGAAAATGATGCACGATCACAAAAGAAAAAAT *F CGAGTGAAGTGCTGTCAGCCTCAGAGAATGAATGCGGTCGCCTTTTACCGATGCCCTACAATGTCCTGAGCTTTGAA *F AGTATTTGTCGAGATTCTTCTAGTCTTGATAGCTGCGAAGTCCTAGATACCTGTGATATCCCTTCAACGCTATTCAC *F AGATGTCGTATTAATAAGCGAAACTGACATGAAACGTTTACAGACAATACTTTGGTTAGAGTTAGCCACAATATTTG *F ATCGCAACAAAGTTTCTTTAGATAAAAGAAAACCTTTTAAGCGTCGTCGCAAAGAAGAGGGTAATCTTTTTGGGGTA *F TCTATAAACGCTCTTATTCGTCGGGATCAGCAAGTTACTGGCACTGACTCATCTTTGGTCCCACTATTTTTGGAAAA *F GCTAATTGGCGAACTTCTGCGACGTGGCTCTAGAGAAGAAGGATTACTTCGAATAGGTGGTCATAAACAAAAGgtta *F taataataaataatgttaataatgaaaataattggtaatacaattttaacaattttctattttcagACTGAATTACT *F TTATAATGAATTAGAATCAACATTTTATCAAAATCCAGATAATCTAGATAACCTCTTTCGCACAGCTACTGTTCATG *F AACTTAGTTCGTTGCTAAAACGATGGCTGCGCGAACTTCCTCAACCTTTGCTTACTAATGAGCTTATACAACTGTTT *F TATCAATGTCACACACTTCCATCAATAGATCAAATGAATGCACTATCGATTTTATGTCACCTGCTTCCGCCTGAAAA *F TAGAAACACATTACGTTCATTATTAAGCTTTTTTAATATTATAATTAATTTAAAAGATATAAACAAAATGAATGTGC *F ATAACGTAGCAACAATAATGGCACCGTCAATGTTTCCACCACGTTATATACATCCGAGTGACAATAACAGCATTGCA *F GAACAAGTAAGAATGGCCGCTCAGTGTTGCCGTTTGACGAATATTTTAATCCTACGTGGCGAAAAACTTTTCCAAGT *F ACCAAACAATTTAATTGTGGAGTCACAGAAAACAATGATGGTATGTGTTATTCTTGAGTTTTTAATTAACCGTAAAT *F ATATATGTTTCCATAGGGTAAGAAAGGATGGCATCGGCATCGGAATTCAAATGAAATTACGGCAAAACCAAGCGGAA *F AGGCGAGCAATGTCGGCGTTGGACACGACTCTACAGTTATAAATAAATACTCAACCAATTTAAAGCATTTACATCCA *F TTTGTTATTTAAACAAACGGCTTCTAACGGTGCTTAAGTTGTATTATATGTTGATAAAATATTTACCTATTATTAAA *F GAAAATATAAAGAATATCCTCTATAAACCGTCAAATTGAAAAAAATGTTCAATTTCAAACCAATTTCAAATGTTCAG *F ATTCAAACCAAAAATTAAGTGAAGCTCAACTGAATAGTTTTGAAAACCCTTTCTAACATTTTTTTTGTTCCTTTTCA *F AATTTTTGATCTTTCGAACTGCTTCAAACTCTACCTATTTTGCTGGTAAAATTATGGAAGAATATATATATT *F translation *F M Q N L I C L L K G T R D S Y T L T K C H S G F P G *F L F T N R Z S L D Q Q F F V C I Q S L K P R F L T *F L N L F N R P T F V N V Y E K N T E T A I Q C V E *F Q S N E I Y L K Q N L R R T P S A P P K S G T Y A D *F I F R G S Q V R C D I P L Y S A D G V E L L G Y S R *F I G T I Q F P R N R S V S D P F C S I G Y V I F N *F N L R T P K G F A F L K L C V F S R S K E S R S E N *F D A R S Q K K K S S E V L S A S E N E C G R L L P M *F P Y N V L S F E S I C R D S S S L D S C E V L D T C *F D I P S T L F T D V V L I S E T D M K R L Q T I L *F W L E L A T I F D R N K V S L D K R K P F K R R R K *F E E G N L F G V S I N A L I R R D Q Q V T G T D S S *F L V P L F L E K L I G E L L R R G S R E E G L L R *F I G G H K Q K \-----------------------------------1-------------------- *F \-------------- T E L L Y N ELESTFYQNPDNLDNLFRTATVHELSSLLKRWLRELPQPLLTNE *F LIQLFYQCHTLPSIDQMNALSILCHLLPPENRNTLRSLLSFFNIIINLKDINKMNVHNVATIMAPSMFPPRYIHPSD *F NNSIAEQVRMAAQCCRLTNILILRGEKLFQVPNNLIVESQKTMMVCVILEFLINRKYICFHRVRKDGIGIGIQMKLR *F QNQ A E R R A M S A L D T T L Q L Z *F This turns out to be a huge complicated gene, starting with the *F annotated CG17082, but extending far further over a section of nnnnns *F Here are the cDNAs that match *F LD04957.5prime AATTCGGCACCAAGGAAAAGAAGTTACAACCTGCTACCCAGTTGATGATTTTTTGTTG *F ATGAAACTAGTAGTTTTGCACATAAGCTGTGTCTAATTTACTTAACTTTTTATAATTAAAAAAAGTGTTTGTTTATT *F TTAATATGAAAAAACAACTAGATATGCGTGTTGTCATGGGTTCTTAGGTATTTTCTCCCATGGATACGAGAAAATTT *F GTTATAAAGAGACGTTTAAAATGAACAGCAATACAGATCTCCATCATTCAGATGATCAGGACTTTTCGGAATTTCTC *F AATGAGTATTATCTGCAAAGCAATTCCCAAAGTATCGAACCTGAAGCAAGTTACGAAGATGGAGAAATGGAAGCAGA *F GTGGCTAGTTTCTGCTGGTTATCCAGAATTGACAAAACCGTTTG *F LD16910.5prime CTGCTACCCAGTTGATGATTTTTTGTTG *F ATGAAACTAGTAGTTTTGCACATAAGCTGTGTCTAATTTACTTAACTTTTTATAATTAAAAAAAGTGTTTGTTTATT *F TTAATATGAAAAAACAACTAGATATGCGTGTTGTCATGGGTTCTTAGGTATTTTCTCCCATGGATACGAGAAAATTT *F GTTATAAAGAGACGTTTAAAATGAACAGCAATACAGATCTCCATCATTCAGATGATCAGGACTTTTCGGAATTTCTC *F AATGAGTATTATCTGCAAAGCAATTCCCAAAGTATCGAACCTGAAGCAAGTTACGAAGATGGAGAAATGGAAGCAGA *F GTGGCTAGTTTCTGCTGGTTATCCAGAATTGACATAACCGTGTGAACAAGGGTTAGA *F LD08837.5prime *F CTTTACACAAACATCTTGATATTT *F GCCTTAGTAAATCTTTAGTGTATAGGTGAAATATACTGAATTTCTGTGTATTTTCTCCCATGGATACGAGAAAATTT *F GTTATAAAGAGACGTTTAAAATGAACAGCAATACAGATCTCCATCATTCAGATGATCAGGACTTTTCGGAATTTCTC *F AATGAGTATTATCTGCAAAGCAATTCCCAAAGTATCGAACCTGAAGCAAGTTACGAAGATGGAGAAATGGAAGCAGA *F GTGGCTAGTTTCTGCTGGTTATCCAGAATTGACAAAACCGTTTGAACAAGGGTTAGAGGTTTCTAAAAAGGACTTGG *F AACCCATACTGACTACTTTATCGAAGCCCCATGCTGAAGCGATTGTACAACTAGTAAGGACTTTAAACAAAACAGTA *F CGGGTGCGCACAAAAAGTCGGCCAAAACGGAAACCTGATATAAGGGATGTATTTCGCGAATTTGATGAACAAGGGAC *F AGTTACACGCTCACGAAGTGCCACTCCGGATTCCCTGGACTCTTTACAAATCGATGAAGCTTGGACCAACAATTCCT *F TACCGACTTTTGTAAATGTATATGAAAAAAATACC *F LD34572.5prime *F TATTTTCTCCCATGGATACGAGAAAATTT *F GTTATAAAGAGACGTTTAAAATGAACAGCAATACAGATCTCCATCATTCAGATGATCAGGACTTTTCGGAATTTCTC *F AATGAGTATTATCTGCAAAGCAATTCCCAAAGTATCGAACCTGAAGCAAGTTACGAAGATGGAGAAATGGAAGCAGA *F GTGGCTAGTTTCTGCTGGTTATCCAGAATTGACAAAACCGTTTGAACAAGGGTTAGAGGTTTCTAAAAAGGACTTGG *F AACCCATACTGACTACTTTATCGAAGCCCCATGCTGAAGCGATTGTACAACTAGTAAGGACTTTAAACAAAACAGTA *F CGGGTGCGCACAAAAAGTCGGCCAAAACGGAAACCTGATATAAGGGATGTATTTCGCGAATTTGATGAACAAGGGAC *F AGTTACACGCTCACGAAGTGCCACTCCGGATTCCCTGGACTCTTTACAAATCGATGAAGCTTGGACCAACAATTCTT *F TACCGACTTTTGTAAATGTATATGAAAAAAATACCGAAACTGCCATACAATGTGTAGAACAATCAAACGAAATATAT *F TTAA *F LD27621.5prime *F CAGATCTCCATCATTCAGATGATCAGGACTTTTCGGAATTTCTC *F AATGAGTATTATCTGGCAAGCAATTCCCAAAGTATCGAACCTGAAGCAAGTTACGAAGATGGAGAAATGGAAGCAGA *F GTGGCTAGTTTCTGCTGGTTATCCAGAATTGACAAAACCGTTTGAACAAGGGTTAGAGGTTTCTAAAAAGGACTTGG *F AACCCATACTGACTACTTTATCGAAGCCCCATGCTGAAGCGATTGTACAACTAGTAAGGACTTTAAACAAAACAGTA *F CGGGTGCGCACAAAAAGTCGGCCAAAACGGAAACCTGATATAAGGGATGTATTTCGCGAATTTGATGAACAAGGGAC *F AGTTACACGCTCACGAAGGNCCACTCCGGATTCCCTGGACTCTTTACAAATCGATGAAGCTTGGACCAACAATTCTT *F TACCGACTTTTGTAAATGTATATGAAAAAAATACCGAAACTGCCATACAATGTGTAGAACAATCAAACGAAATATAT *F TTAAAACAAAACCTGCGACGAACCCCTAGCGCCCCGCCTAAAAGCGGCACCTATGCGGATATATTTC *F LD04154.5prime *F TGGCTAGTTTCTGCTGGTTATCCAGAATTGACAAAACCGTTTGAACAAGGGTTAGAGGTTTCTAAAAAGGACTTGG *F AACCCATACTGACTACTTTATCGAAGCCCCATGCTGAAGCGATTGTACAACTAGTAAGGACTTTAAACAAAACAGTA *F CGGGTGCGCACAAAAAGTCGGCCAAAACGGAAACCTGATATAAGGGATGTATTTCGCGAATTTGATGAACAAGGGAC *F AGTTACACGCTCACGAAGTGCCACTCCGGATTCCCTGGACTCTTTACAAATCGATGAAGCTTGGACCAACAATTCTT *F TACCGACTTTTGTAAATGTATATGAAAAAAATACCGAAACTGCCATACAATGTGTAGAACAATCAAACGAAATATAT *F TTAAAACAAAACCTGCGACGAACCCCTAGCGCCCCGCCTAAAAGCGGCACCTATGCGGATATATTTCGTGGTTCGCA *F GTGCGATGTGATATACCTTTGTA *F LD15784.5prime *F AAAAGTCGGCCAAAACGGAAACCTGATATAAGGGATGTATTTCGCGAATTTGATGAACAAGGGAC *F AGTTACACGCTCACGAAGGTCCACTCCGGATTCCCTGGACTCTTTACAAATCGATGAAGCTTGGACCAACAATTCTT *F TACCGACTTTTGTAAATGTATATGAAAAAAATACCGAAACTGCCATACAATGTGTAGAACAATCAAACGAAATATAT *F TTAAAACAAAACCTGCGACGAACCCCTAGCGCCCCGCCTAAAAGCGGCACCTATGCGGATATATTTCGTGGTTCGCA *F AGTGCTATGTGATATACCTTTGTACTCGGCGGATGGTGTAGAATTGCTTGGATATTCACGGATTGGCACCATACAAT *F TTCCTCGGAACCGATCCGTCTCTGATCCATTTTGTTCTATTGGTCGATCAAAGGAGTCAAGAAGTGAAAATGATGCA *F CGATCACAAAAGAAAAAATCGAGTGAAGTGCTGTCAGCCTCAGAGAATGAATGCGGTCGCCTTTTACCGATGCCCTA *F CAATGTCCTGAGCTTTGAAAGTATTTGTCGAGATTCTTCTAGTCTTGATAGCTGCGAGTCCTAGATACCTGTGATAT *F CCCTTCAACGCTATTCACAGATGTCGTATTAATAAGCGAAACTGACATGAAACGTTTACAGACAATACTTTGGTTAG *F AGTTAG *F Contig AATTCGGCACCAAGGAAAAGAAGTTACAACCTGCTACCCAGTTGATGATTTTTTGTTG *F ATGAAACTAGTAGTTTTGCACATAAGCTGTGTCTAATTTACTTAACTTTTTATAATTAAAAAAAGTGTTTGTTTATT *F TTAATATGAAAAAACAACTAGATATGCGTGTTGTCATGGGTTCTTAGGTATTTTCTCCCATGGATACGAGAAAATTT *F GTTATAAAGAGACGTTTAAAATGAACAGCAATACAGATCTCCATCATTCAGATGATCAGGACTTTTCGGAATTTCTC *F AATGAGTATTATCTGCAAAGCAATTCCCAAAGTATCGAACCTGAAGCAAGTTACGAAGATGGAGAAATGGAAGCAGA *F GTGGCTAGTTTCTGCTGGTTATCCAGAATTGACAAAACCGTTTGAACAAGGGTTAGAGGTTTCTAAAAAGGACTTGG *F AACCCATACTGACTACTTTATCGAAGCCCCATGCTGAAGCGATTGTACAACTAGTAAGGACTTTAAACAAAACAGTA *F CGGGTGCGCACAAAAAGTCGGCCAAAACGGAAACCTGATATAAGGGATGTATTTCGCGAATTTGATGAACAAGGGAC *F AGTTACACGCTCACGAAGGTCCACTCCGGATTCCCTGGACTCTTTACAAATCGATGAAGCTTGGACCAACAATTCTT *F TACCGACTTTTGTAAATGTATATGAAAAAAATACCGAAACTGCCATACAATGTGTAGAACAATCAAACGAAATATAT *F TTAAAACAAAACCTGCGACGAACCCCTAGCGCCCCGCCTAAAAGCGGCACCTATGCGGATATATTTCGTGGTTCGCA *F AGTGCTATGTGATATACCTTTGTACTCGGCGGATGGTGTAGAATTGCTTGGATATTCACGGATTGGCACCATACAAT *F TTCCTCGGAACCGATCCGTCTCTGATCCATTTTGTTCTATTGGTCGATCAAAGGAGTCAAGAAGTGAAAATGATGCA *F CGATCACAAAAGAAAAAATCGAGTGAAGTGCTGTCAGCCTCAGAGAATGAATGCGGTCGCCTTTTACCGATGCCCTA *F CAATGTCCTGAGCTTTGAAAGTATTTGTCGAGATTCTTCTAGTCTTGATAGCTGCGAGTCCTAGATACCTGTGATAT *F CCCTTCAACGCTATTCACAGATGTCGTATTAATAAGCGAAACTGACATGAAACGTTTACAGACAATACTTTGGTTAG *F AGTTAG *F Put these together to encode a 296aa protein at 14% serine. Seems *F good, but too hard to put all the introns together here. *F \*****************A. mellifera BB260019B20F2.F *F GGATCGTTATCCTTTCAATGACGAATAATTATTCTAAAATCGTATCAAAATTACTAACGAGTCAAAAATTTTACCTG *F TGCTCGAGAAACGCGCGTAGTATAAATAAAATTGGACGGATATCCTTTCAATGACGAATAATTATTCTAAAATCAAA *F ATTAACGAGTCAAAAATAAGCGTTGAAAGAGAAAGAAAATTTTAAGAGGAGAATGTAAATGGAATCGATAGATGACG *F GTGGCTGTCACGATGCTGCAAGGTCGACCGGAAGCGAGACGGGGTGTTCAATTTCCGCCGAATGAAACGAAGCTGTG *F CCCGTTTCAGGAACTCGCGCACAAGCTCTCGCGGAGCTTCGACATGCAGGAGGCTCAGCTCCTCGAGGAGGGTGGCT *F CTGGTGCCGCTACGGCGGGTGCTGGGGGCGCGGGTGGCCCGCCACGAAGACATCACAGCGCTCAGAGATTGGCCAGG *F AGCGAAATATCTGAGAGAAGGGAAGAGGACGGAGCGTTGGTGGTACCCGATCACCAGGGCAACCTGAGGATCACCGT *F GAAGAAGACCAAGTCGATTTTGGGCATTGCCATCGAGGGCGGTGCCAATACCAAACATCCACTGCCCAGGATCATCA *F ATATACACGATAATGGGGCAGCTTACGAGGCAGGTGGCCTCGAGGTCGGTCAACTGATCCTAGAAGTCGATGGACAC *F AAAGTGGAAGGTCTGCATCATCAGGAGGTGGCAAGACTGATCGCAGAGTCGTTCGCGAGGCGCGATCGCAACG *F long ORF after long 5' UTR encodes 11% G/A protein; weak but convincing *F BLASTX matches to several Drosophila proteins; especially CG7151 at *F e-05; strangely there is a BLASTX match to KIAA1526 protein [Homo *F sapiens] which is far longer and better at 32% over 175aa; e-18; *F genomic match is even better at 80% to unannotated region; NEW GENE!; *F but no ESTs? Most of it is a PDZ domain. *F There are three versions of the human protein annotated, 500, 700 and *F 900aa long, and in each we have the C-terminus, so perhaps our cDNA is *F unspliced? *F AE003536.2 tcgaaaccgaaactaaaattaaatgaataggagttggccacaaaactctcaaga *F agtttcgatATGAAGGAAGAAGGCGGCACATTGCTGGGCGATAAAGGTGTACGAAGGCATCAGgtgagtgctactcg *F aaaatagtgcaagtacactaaataaacagatatctatatcaacaaataataactgaatacagcacttgaatgccaac *F aagagaaaccagtatacgtttcagctcgaaattaacagagttaaatcatattatcataagactctcgtcggtttttc *F tattaaattctatacaattcggtcaccccagaacatttcatgattcattccaaacaaatcacacaaaagtttctact *F ttctatctctctaattctctttatacctaactcaaaaataaacagcaactgtaccattgattattttaaactagcaa *F tatactatgtaaatttgaattggcctaccaatagcttgaaccgtttcctattgtttgaacccacacgtaattaagta *F caagaacccaaaacagtatacatatacaaattccttgaatcaaccaattgttaaattgcttgcctttaccaaatcac *F agaatctattttatcagcttgaaccaagttacttttaatcaaaccaatacacaatttgaattgtaagctaatcctag *F aagtaaagccaatggaatatttttgatcaaaccggaaaaacctactaaaatctttaatttagtttaaagatttttca *F tgaacttggcctatttgtttttcagTCCATGCAGCGTCTGTCAGCGGAGCAGAATGGTGGTTCAACGACTGAACAAA *F CACATGAACACAATCCAAACGTCGTACCTGATCATAGAGGCAACTTACACATTACAGTTAAGAAAACCAAACCAATT *F TTAGGTATTGCTATCGAAGGTGGTGCTAATACAAAACACCCGCTCCCTAGGATAATCAATATCCATgtaagtacgca *F atagatcataaaatgactttttgagactcaaatgcctggcaaatagGAAAATGGTGCAGCATTTGAAGCGGGCGGCT *F TAGAAGTCGGCCAACTCATCCTGGAGGTAGATGGAACGAAAGTGGAGGGTCTGCATCATCAGgtatgttcgagctcc *F cttttttttttgagttattaatttgacggttttctttttgattttccagGAGGTTGCTCGACTAATAGCCGAATGCT *F TTGCTAATCGTGAAAAGGCTGAAATAACCTTCTTAGTTGTCGAAGCAAAAAAATCAAATTTGGAACCGAAGCCGACG *F GCGCTGATATTTTTAGAAGCCTAAcatttgcttgccctgccggccagtgacccattggaacgacaaaaactcgagtt *F cctttacgaatggggcatcgatttaacagagacgccaaaaccaatgccaacaccaataccgctaacgaaagccaaga *F atccgccgccactgccccatgagttgcacaacaacatcaacagccagtatggtagcagtgcggctctcagcaatcat *F caacctcatcagcatacgcatccacatccccagcagcagcagcagcaacaacaacattcaaacacaaaaacgcccaa *F cacgaacagcaacaaaacacaaggaacaccaacaacgggaacgggagctgcaacaactggcagcaaacaacaacagc *F aaccgggaaacaccaccaacacaccaacgaaggcgtcgcgtgaggcgactcccacaagggagcagcatc *F translation *F M K E E G G T L L G D K G V R R H Q \----------------0------- *F \------------------------0-------------------------------0-------------------- *F \-----------0-------------------------------0-------------------------------0- *F \------------------------------0-------------------------------0-------------- *F \-----------------0-------------------------------0--------------------------- *F \----0-------------------------------0-------------------------------0-------- *F \-----------------------0-------------------------------0--------------------- *F \----------0-------------------------------0-------------------------------0-- *F \-----------------------------0-------------------------------0--------------- *F \--------------- S M Q R L S A E Q N G G S T T E Q T H E H *F N P N V V P D H R G N L H I T V K K T K P I L G I *F A I E G G A N T K H P L P R I I N I H \--------------------- *F \-------0---------------------------- E N G A A F E A G G L E V G *F Q L I L E V D G T K V E G L H H Q \------------------------0 *F \--------------------------------------- E V A R L I A E C F A N R *F E K A E I T F L V V E A K K S N L E P K P T A L I *F F L E A Z *F This is highly speculative, and there's plenty of room for more 5' *F Drosophila *F MKEEGGTLLGDKGVRRHQSMQRLSAEQNGGSTTEQTHEHNPNVVPDHRGNLHITVKKTKPILGIAIEGGANTKHPLP *F RIINIHENGAAFEAGGLEVGQLILEVDGTKVEGLHHQEVARLIAECFANREKAEITFLVVEAKKSNLEPKPTALIFL *F EAZ *F Honey bee from below *F MPRTRAGCRRTPQHQVTTSDLALSNDDECDNQDYEDELENGRGRRHSSPGGSRGNPRDYGHHHLHPDNLELAHKLS *F RSFDMQEAQLLEEGGSGAATAGAGGAGGPPRRHHSAQRLARSEISERREEDGALVVPDHQGNLRITVKKTKSILGIA *F IEGGANTKHPLPRIINIHDNGAAYEAGGLEVGQLILEVDGHKVEGLHHQEVARLIAESFARRDRNEIEFLVVEAKKS *F NLEPKPTALIFLEA *F \****************extras \- the first 90kb of AE003536.2 above is unannotated \- *F but some possible ORFS in it, so try BLASTX searches, and find NOTHING! Trul *F y 90kb of nothing! *F \****************A. mellifera BB260020B10C4.F GGCATCCAGGATAGAGCAGCGACGATATCGC *F AATCGAGAGAGTGCAATCGATGAATGTCATCAAGAAGAATGACCAACTGTTCCTTTATAGGTTCCTCCTCGATCCGT *F CTCATAATTTGCCCCAGCCAATTGTTCAAATAAAGAGGATCAAACGAGGCGTCTTTGGGAAGATTTCCATCTAACGA *F GCCGGAAAGGAAGCCGACGTGTTGACAGAGTACGCGAAGCAGTTCGAGACTATACGCTGATCGAGGTGTAGACGCAC *F AGAGGCGAACAATCCTGAATGTGGGACAATCCAACCAGGTGGAAGCATCCTCGTAAATTCTTGACAGCAACGATGAT *F TTCCCGGAATATTTACCGCCTTTTATCAATATGGGGCCATGCTTCTGTGATTTACCCGATATTAAGATCTCCCTGAT *F CCTTTCAATATTCTCGTTGTTCTCGCAGGGTTTAATCTCCCTAAGAAGCGCCAGATGTACCAGACTCTCCGCATAGA *F TTTCCTGCACCATCTTCTTCTTGCTCTTGATTTCTGGATCCGCTTCTATAGATGCGTTCACCAAATTCTGCACGCGA *F TTCACCACCAAGCGACAAAGATGAGCCAGGTAGCCTTTATGGGATCTATTATCGGGATTAATGCCGCCTTGCTTCTG *F ATCCACATCGATGTTAATTACATTGTCGGATGGGAGACGGTTGTCCACCAAAGACTTCAACGAGGCAACTGCTCTTG *F TGCTCTTTTTGCCTTTCCATGTGCGTTTCACGGCTATTATGCCGTCACCTGTTTCG *F full ORF on RC encodes 260aa 13% leucine protein; only very weak BLASTX *F matches to Drosophila proteins; nothing more in nr; *F genomic match is near N-terminus of 1240aa protein BcDNA:GH04922 gene *F product; with two ESTs, so could be alternative splice? *F In fact end of this translation overlaps N-terminus of this protein, so *F is probably even longer. *F Also, weakly matches C. elegans T05C3.2 , which is 2340aa, from *F 900-1150, so Drosphila protein could be a lot longer. *F In fact, connects CG17671 to BcDNA:GH04922, but the two Drosophila ESTs *F are only for the start of the latter. I will not try to figure it all *F out. *F \*****************A. mellifera BB260022B10E6.F ATGCCCAGGACCAGAGCTGGGTGCAGGAGG *F ACGCCGCAGCACCAAGTGACCACCTCCGATCTCGCCCTCTCCAACGACGACGAGTGCGACAACCAGGATTACGAGGA *F CGAGTTGGAGAACGGGCGTGGACGTAGGCACAGCTCGCCAGGTGGCTCGAGGGGGAACCCCAGGGATTACGGCCATC *F ATCATCTTCATCCGGACAATTTGGAACTCGCGCACAAGCTCTCGCGGAGCTTCGACATGCAGGAGGCTCAGCTCCTC *F GAGGAGGGTGGCTCTGGTGCCGCTACGGCGGGTGCTGGGGGCGCGGGTGGCCCGCCACGAAGACATCACAGCGCTCA *F GAGATTGGCCAGGAGCGAAATATCTGAGAGAAGGGAAGAGGACGGAGCGTTGGTGGTACCCGATCACCAGGGCAACC *F TGAGGATCACCGTGAAGAAGACCAAGTCGATTTTGGGCATTGCCATCGAGGGCGGTGCCAATACCAAACATCCACTG *F CCCAGGATCATCAATATACACGATAATGGGGCAGCTTACGAGGCAGGTGGCCTCGAGGTCGGTCAACTGATCCTAGA *F AGTCGATGGACACAAAGTGGAAGGTCTGCATCATCAGGAGGTGGCAAGACTGATCGCAGAGTCGTTCGCGAGGCGCG *F ATCGCAACGAGATCGAGTTCCTGGTGGTCGAGGCGAAAAAAAGCAACCTGGAGCCGAAGCCGACAGCGCTGATATTC *F CTGGAGGCGTAGCAGGAATCTCCCACCTCCGAGCCGGAACCACCGTAGCCCAACCTGACCAGAGCTCGGCCCGCGAC *F ACACTTGGAGCGTGTCGACGCTGAACCGACGTCATGAGAAG *F long ORF encodes G/A rich protein with only weak BLASTX match to *F several Drosophila proteins; 46% e-20 match to KIAA1526 protein [Homo *F sapiens]; *F genomic match is 85% to at least three exons in unannotated region; NEW *F GENE; no ESTs *F This protein has a PDZ domain, so could be same as BB260019B20F2.F? *F Indeed it is. But differ in the 5' regions, which is probably an *F intron in that one. *F >BB260019B20F2.F 766 0 766 ABI GGATCGTTATCCTTTCAATGACGAATAA *F TTATTCTAAAATCGTATCAAAATTACTAACGAGTCAAAAATTTTACCTGTGCTCGAGAAACGCGCGTAGTATAAATA *F AAATTGGACGGATATCCTTTCAATGACGAATAATTATTCTAAAATCAAAATTAACGAGTCAAAAATAAGCGTTGAAA *F GAGAAAGAAAATTTTAAGAGGAGAATGTAAATGGAATCGATAGATGACGGTGGCTGTCACGATGCTGCAAGGTCGAC *F CGGAAGCGAGACGGGGTGTTCAATTTCCGCCGAATGAAACGAAGCTGTGCCCGTTTCAGGAACTCGCGCACAAGCTC *F TCGCGGAGCTTCGACATGCAGGAGGCTCAGCTCCTCGAGGAGGGTGGCTCTGGTGCCGCTACGGCGGGTGCTGGGGG *F CGCGGGTGGCCCGCCACGAAGACATCACAGCGCTCAGAGATTGGCCAGGAGCGAAATATCTGAGAGAAGGGAAGAGG *F ACGGAGCGTTGGTGGTACCCGATCACCAGGGCAACCTGAGGATCACCGTGAAGAAGACCAAGTCGATTTTGGGCATT *F GCCATCGAGGGCGGTGCCAATACCAAACATCCACTGCCCAGGATCATCAATATACACGATAATGGGGCAGCTTACGA *F GGCAGGTGGCCTCGAGGTCGGTCAACTGATCCTAGAAGTCGATGGACACAAAGTGGAAGGTCTGCATCATCAGGAGG *F TGGCAAGACTGATCGCAGAGTCGTTCGCGAGGCGCGATCGCAACG *F ATGCCCAGGACCAGAGCTGGGTGCAGGAGGACGCCGCAGCACCAAGTGACCACCTCCGATCTCGCCCTCTCCAACG *F ACGACGAGTGCGACAACCAGGATTACGAGGACGAGTTGGAGAACGGGCGTGGACGTAGGCACAGCTCGCCAGGTGGC *F TCGAGGGGGAACCCCAGGGATTACGGCCATCATCATCTTCATCCGGACAATTTG----------------------- *F \----------------------------------------------------------------------------- *F \-----------------------------------------------------------GAACTCGCGCACAAGCTC *F TCGCGGAGCTTCGACATGCAGGAGGCTCAGCTCCTCGAGGAGGGTGGCTCTGGTGCCGCTACGGCGGGTGCTGGGGG *F CGCGGGTGGCCCGCCACGAAGACATCACAGCGCTCAGAGATTGGCCAGGAGCGAAATATCTGAGAGAAGGGAAGAGG *F ACGGAGCGTTGGTGGTACCCGATCACCAGGGCAACCTGAGGATCACCGTGAAGAAGACCAAGTCGATTTTGGGCATT *F GCCATCGAGGGCGGTGCCAATACCAAACATCCACTGCCCAGGATCATCAATATACACGATAATGGGGCAGCTTACGA *F GGCAGGTGGCCTCGAGGTCGGTCAACTGATCCTAGAAGTCGATGGACACAAAGTGGAAGGTCTGCATCATCAGGAGG *F TGGCAAGACTGATCGCAGAGTCGTTCGCGAGGCGCGATCGCAACGAGATCGAGTTCCTGGTGGTCGAGGCGAAAAAA *F AGCAACCTGGAGCCGAAGCCGACAGCGCTGATATTCCTGGAGGCGTAGCAGGAATCTCCCACCTCCGAGCCGGAACC *F ACCGTAGCCCAACCTGACCAGAGCTCGGCCCGCGACACACTTGGAGCGTGTCGACGCTGAACCGACGTCATGAGAAG *F See above file. The N-terminus encoded by the 5' part of this contig *F does not have a Drosophila match, but rest is clearly a good match, so *F unclear which one is right or wrong. *F \******************A. mellifera BB260023A20H5.F TGTACGCGCATGTGACAAATAGAAAAGAA *F TTTATTTCTTATTTTTTTTATATTTTTTTTTTCTTACAATGAATCAATATAATATAATATCTATATAAAAATTTATT *F AAAAGATGGAATTCATTAATTTTAAAATATATACGATAGTGATTATAAGCCTCTATTGTTTGGGCGTTGTCGGACAA *F TATGAGTGGCAAGCTAGAGATGCTTTTGATGAAATCCGTTTAAAAATGGATAAGATTAATGAAGAGAATTGTCCTAT *F TCAACATATCGGAGACCTCTATCTACCGGAAGATACAGTCTCTCATTTACCTGACATTAAAGATATTAATATCAATC *F CTGTATTTCCAAATAGAACTGCTTTACTTCATCTTCATAATATGGCTCTTAGTAGATCATTTTTTTGGAGTTATATT *F TTACAATCTCGATTCATACGTCCAGCTATCAATGATACTTATGATCCAGGCATGATGTATTATTTTTTATCAACAGT *F TGCTGATGTTTCTGCAAATTCACATATAAATGCTTCTGCTATATATTTCTCACCAAATATGTCTTATTCTTCATCAT *F ATAGAGGTTTTTTTAATAAAACTATGCCCAGATTTGCTCCGAGAACTTTTAGAGCTGATGATNTTAATGATCCTATA *F CATTTAGAAAGAATATCCACAAGAAATACATTTAATGTGCAAGATCTTGGGGCATTTGCCAGTGGTAGTCTTGGTGA *F AGATTATACAACAGATTATTATCGTATAAATGAA *F Long complete ORF encodes secreted 180aa 12% isoleucine protein; *F genomic match is 80% full-length e-108 to single exon is unannotated *F region; NEW INSECT GENE; two ESTs *F From 6-42kb is empty in this accession! Check for other genes too. *F AE003732.2 RC ATATATAGAAGCAATAGCATTGAAATAGCAACATTCATTTTTTGTATTAATGTT *F CGATATCAGCCAGCAACCAATATAAACAATATATCGATACTATCGGACAGGTGGCCCATCTCTGTTTGAGGGCGTAG *F TTCCAACAAGTGCTGAGCATCACAATTTTCTATTACTAAGCCCAGCTTTGCGTTGGCGCGCCCCAGAATCTCATTTT *F ATATTTAGTTTCTGCCAGTTTAGTTAATTAGTTAGTTGATAGTGTTGTTTGTTTCTTCTGCAACAATTGTGTGCGAT *F AGGAGTCGGGCAAAATGTTCCCGTCGTCGATTTTGGGGCGCAGCTATTTGCTTTTTATGCTGGTGCTCGCCGTGGGC *F GTGTTCGCCCAACACGAGTGGCAGGCCCGGGATGCCTTTGATGAGATAAAGAGGCAGTTCGACAAGGTGAACGCGGA *F TAACTGCCCCATCCAACACCATTCGGACCTTTTCATGCCCATGGACGCGGTGTCCCACAAGCCGGACATCAAGGAGA *F TCAACGTGAATCCGGTGTTCCCCAACCGAACTGCCCTGCTGCATCTGCAGAATATGGCCCTTAGCAGAAGCTTCTTC *F TGGAGCTACATCCTCCAGTCGAGGTTTATTCGACCCGCCATCAACGACACCTACGATCCCGGCATGATGTACTACTT *F TCTGTCCACCGTAGCCGATGTATCCGCCAACCCACATATCAACGCCTCGGCCGTGTACTTCTCCCCCAACAGCTCGT *F ATTCGTCGTCGTATCGCGGCTTCTTCAATAAGACGTTCCCCAGATTCGGGCCAAGAACCTTCAGGCTGGACGACTTC *F AACGATCCCATTCATCTGCAGAAGATATCGACGTGGAATACTTTCGATGTTCAGGATCTGGGCGCCCATCACCCGGA *F CTCCATATCCAAGGACTACACCCACGACCTGTATAAAATAAACGAGTGGTACCGCGCCTGGCTACCAGACAACGTCG *F AGGGACGGCACGATACGAAGATCACCTACCAGGTGGAAATCCGCTATGCGAACAACACAAACGAGACGTATACCTTC *F CACGGACCGCCTGGCTCTGAAGAAAACCCTGGTCCGATTAAATTTACAAGGCCGTACTTCGATTGTGGCAGGTCCAA *F CAAGTGGCTGGTGGCCGCAGTAGTGCCAATTGCGGATATCTACCCCCGACACACGCAGTTCCGTCACATTGAGTATC *F CCAAgtaagataccttgaatatcccctgaataccctccttttatctactgtatcgcttttagATACACGGCCGTTTC *F GGTTCTTGAGATGGACTTCGAGCGTATCGACATAAACCAGTGTCCATTGGGTGAAGGCAACAAAGGACCTAATCACT *F TTGCGGATACGGCGCGGTGTAAAAAAGAAACGACAGAGTGTGAACCATTACAAGGCTGGGGCTTTAGGCGCGGTGGC *F TACCAGTGCCGTTGTAAGCCAGGTTTTCGGCTGCCCAACGTAGTGCGGCGACCTTATCTGGGCGAGATTGTGGAGCG *F CGCATCGGCAGAACAGTACTACAACGAGTACGACTGCCTTAAGATTGGCTgtatgttttaagtagcaatatgtaaaa *F gtatgagatttgactcttgatgtttttttttagGGATCCAAAAGCTTCCCATTCAGTGGGATAAGGCCTCCTACCAC *F ATTCGCCAAAAGTATCTGGACCGGCATCCGGAATATCGCAACTACACCACCGGCTCGCGATCACTTCATGCTGAGCA *F CTTAAATATTGATCAGGCGTTGAAGTATATTCATGGAGTCAACTATCGCACTTGCAAAAAgtaagacacatacaaaa *F cttatccagccaaggtcacttcaataaactgatcaattatgctatcgccttgacagCTTCCATCCGCAGGATCTGAT *F TCTTCGCGGTGATGTGAGCTTCGGCGCCAAGGAGCAGTTCGAGAACGAAGCCAAGATGGCCGTGAGACTGGCCAACT *F TTATTAGCGCCTTTCTGCAGgtaagcaaacgattcagagcaaaggattcccatcgccttcacgctaaatgaagagca *F ataattgataacccgacacctattaagagccttcgacgacggctcttgaaaacttctcaagtgtaaattataatttt *F ccacgcgtaattcaacttcctcgaatttcctgcattgccagtttctcggttcttaccgatgctgct *F LD18575.5prime *F CTCCGGTTTGAGGGCGTAG *F TTCCAACAAGTGCTGAGCATCACAATTTTCTATTACTAAGCCCAGCTTTGCGTTGGCGGGCCCAAGAATCTCATTTT *F ATATTAAGTTTCTGCAAGTTTAGTTAATTAGTTAGTTGATAGTGTTGTTTGTTTCTTCTGCAACAATTGTGTGCGAT *F AGGAGTCGGGCAAAATGTTGCCGTCGTCGATTTTGGGGCGCAG-TATTTGCTTTTTATGCTGGTGCTCGCCGTGGGG *F CTGTTCGCCATACACGAGTGGCAGGCCCGGGATGCCTTTGATGAGATAAAGAGGCAGTTCGACAAGGTGAACGCGGA *F TAACTGCCCCATCCAACACCATTCGGACCTTTTCATGCCCATGGACGCGGTGTCCCACAAGCCGGACATCAAGGAGA *F TCAACGTGAATCCGGTGTTCCCCAACCGAACTGCCCTGCTGCATCTGCAGAATAT *F LD21417.5prime *F CTAAGCT---NTNTGCGTTGGCGCGCCCCAGAATCTCATTTT *F ATATTTAGTTTCTGCCAGTTTAGTTAATTAGTTAGTTGATAGTGTTGTTTGTTTCTTCTGCAACAATTGTGTGCGAT *F AGGAGTCGGGCAAAATGTTCCCGTCGTCGATTTTGGGGCGCA-CTATTTGCTTTTTATGCTGGTGCTCGCCGTGGGC *F \-TGTTCGCC-AACACGAGTGGCAGGCCCGGGATGCCTTTGATGAGATAAAGAGGCAGTTCGACAAGGTGAACGCGGA *F TAACTGCCCCATCCAACACCATTCGGACCTTTTCATGCCCATGGACGCGGTGTCCCACAAGCCGGACATCAAGGAGA *F TCAACGTGAATCCGGTGTTCCCCAACCGAACTGCCCTGCTGCATCTGCAGAATATGGCCCTTAGCAGAAGCTTCTTC *F TGGAGCTACATCCTCCAGTCGAGGTTTATTCGACCCGCCATCAACGACACCTACGA *F LD13768.5prime *F CGCCCATCACCCGGA *F CTCCATATCCAAGGACTACACCCACGACCTGTATAAAATAAACGAGTGGTACCGCGCCTGGCTACCAGACAACGTCG *F AGGGACGGCACGATACGAAGATCACCTACCAGGTGGAAATCCGCTATGCGAACAACACAAACGAGACGTATACCTTC *F CACGGACCGCCTGGCTCTGAAGAAAACCCTGGTCCGATTAAATTTACAAGGCCGTACTTCGATTGTGGCAGGTCCAA *F CAAGTGGCTGGTGGCCGCAGTAGTGCCAATTGCGGATATCTACCCCCGACACACGCAGTTCCGTCACATTGAGTATC *F CCAA----------------------------------------------------------ATACACGGCCGTTTC *F GGTTCTTGAGATGGACTTCGAGCGTATCGACATAAACCAGTGTCCATTGGGTGAAGGCAACAAAGGACCTAATCACT *F TTGCGGATACGGCGCGGTGTAAAAAAGAAACGACAGAGTGTGAACCATTACAAGGCTGGGGCTTTAGGCGCGGTGGC *F TACCAGTGCCGTTGTAAGCCAGGTTTTCGGCTGCCCAACGTAGTGCGGCGACCTTATCTGGGCGAGATTGTGGAGCG *F CCCATCGGCAGAACAGTACTACAACGAGTACGACTGCCCTAAGATTGGCT--------------------------- *F \---------------------------------GGAT *F LD16802.5prime *F CGCCCATCACCCGGA *F CTCCATATCCAAGGACTACACCCACGACCTGTATAAAATAAACGAGTGGTACCGCGCCTGGCTACCAGACAACGTCG *F AGGGACGGCACGATACGAAGATCACCTACCAGGTGGAAATCCGCTATG-GAACAACACAAACGAGACGTATACCTTC *F CACGGACCGCCTGGCTCTGAAGAAAACCCTGGTCCGATTAAATTTACAAGGCCGTACTTCGATTGTGGCAGGTCCAA *F CAAGTGGCTGGTGGCCGCAGTAGTGCCAATTGCGGATATCTACCCCCGACACACGCAGTTCCGTCACATTGAGTATC *F CCAA----------------------------------------------------------ATACACGGCCGTTTC *F GGTTCTTGAGATGGACTTCGAGCGTATCGACATAAACCAGTGTCCATTGGGTGAAGGCAACAAAGGACCTAATCACT *F TTGCGGATACGGCGCGGTGTAAAAAAGAAACGACAGAGTGTGA *F HL02444.5prime *F CK00408.5prime *F GH23994.5prime *F Translation *F M F P S S I L G R S Y L L F M L V L A V G *F V F A Q H E W Q A R D A F D E I K R Q F D K V N A D *F N C P I Q H H S D L F M P M D A V S H K P D I K E *F I N V N P V F P N R T A L L H L Q N M A L S R S F F *F W S Y I L Q S R F I R P A I N D T Y D P G M M Y Y F *F L S T V A D V S A N P H I N A S A V Y F S P N S S *F Y S S S Y R G F F N K T F P R F G P R T F R L D D F *F N D P I H L Q K I S T W N T F D V Q D L G A H H P D *F S I S K D Y T H D L Y K I N E W Y R A W L P D N V *F E G R H D T K I T Y Q V E I R Y A N N T N E T Y T F *F H G P P G S E E N P G P I K F T R P Y F D C G R S N *F K W L V A A V V P I A D I Y P R H T Q F R H I E Y *F P K----------------------------------2----------------------- Y T A V S *F V L E M D F E R I D I N Q C P L G E G N K G P N H *F F A D T A R C K K E T T E C E P L Q G W G F R R G G *F Y Q C R C K P G F R L P N V V R R P Y L G E I V E R *F A S A E Q Y Y N E Y D C L K I G \--------------------------- *F \-------1-------------------------W I Q K L P I Q W D K A S Y H *F I R Q K Y L D R H P E Y R N Y T T G S R S L H A E H *F L N I D Q A L K Y I H G V N Y R T C K N----------------- *F \---------------------2---------------------------------- F H P Q D L I *F L R G D V S F G A K E Q F E N E A K M A V R L A N *F F I S A F L Q \-----------------------0--------------------------------- *F \---------0------------------------------------------0------------------------ *F \------------------0------------------------------------------0---- *F fly *F MFPSSILGRSYLLFMLVLAVGVFAQHEWQARDAFDEIKRQFDKVNADNCPIQHHSDLFMPMDAVSHKPDIKEINVNP *F VFPNRTALLHLQNMALSRSFFWSYILQSRFIRPAINDTYDPGMMYYFLSTVADVSANPHINASAVYFSPNSSYSSSY *F RGFFNKTFPRFGPRTFRLDDFNDPIHLQKISTWNTFDVQDLGAHHPDSISKDYTHDLYKINEWYRAWLPDNVEGRHD *F TKITYQVEIRYANNTNETYTFHGPPGSEENPGPIKFTRPYFDCGRSNKWLVAAVVPIADIYPRHTQFRHIEYPKYTA *F VSVLEMDFERIDINQCPLGEGNKGPNHFADTARCKKETTECEPLQGWGFRRGGYQCRCKPGFRLPNVVRRPYLGEIV *F ERASAEQYYNEYDCLKIGWIQKLPIQWDKASYHIRQKYLDRHPEYRNYTTGSRSLHAEHLNIDQALKYIHGVNYRTC *F KNFHPQDLILRGDVSFGAKEQFENEAKMAVRLANFISAFLQSMQTITRISSLQVSDPNEVYSGKRVADKPLTEDQMI *F GETLAIVLGDSKVWSATMLWERNKFTNRTYFAPYAYKTELNTRKFKVEDLARLNKTHELYTEKKYFKFLKQRWNTNF *F DDLETFYMKIKIRHNETGEYQQKYEHYPNSYRAANIKHGYWTQPQFDCDGYVKKWLVTYAVPFFGWDSLKVKLEFKG *F VVAVSMDMLQLDINQCPDWYYEPNAFKNTHKCDEQSSYCVPIMGRGYETGGYKCECLQGYEYPFEDLITYYDGQLVE *F AEYQNIVADVETRYDMFKCRLAGASGLQSALGLVVALIGLTLTLLYRFS *F honeybee *F MEFINFKIYTIVIISLYCLGVVGQYEWQARDAFDEIRLKMDKINEENCPIQHIGDLYLPEDTVSHLPDIKDININPV *F FPNRTALLHLHNMALSRSFFWSYILQSRFIRPAINDTYDPGMMYYFLSTVADVSANSHINASAIYFSPNMSYSSSYR *F GFFNKTMPRFAPRTFRADDLMILYI-RKNIHRNTFNVQDLGAFASGSLGEDYTTDYYRINE *F Amazing thing is that I can continue this gene for 3500bp with *F perfectly predicted introns and lovely exons, encoding over 800aa! *F Possible TM domain at end, if real. *F The only similar protein in NR is CG18679, which is 179aa and has a *F 5C5G region that matches twice in this one, presumably a extra-cellular *F disulfide-bonded domain. *F Also TBLASTN matches in 75-80% range for an A. gambiae and B. mori EST *F each. So is insect specific \- yet quite conserved! *F And now find multiple ESTs confirming beginning and end and several *F introns. *F At end, seems there may be another gene further on this strand, with *F some ORFs and good intron splices; *F But there are ESTs for genes on opposite strand, although could be *F within intron of this gene (other was around, see below) *F The honeybee EST translation could also be continued in alignment with *F two frameshifts, so indeed is simply N-terminus of this long gene. *F \****************A. mellifera The remaining 3kb at the end of this gene *F before the next annotation is strange. *F As noted above, one might be able to construct a gene going in the same *F RC direction as our new one, but there are about 30 Drosophila ESTs *F from all tissues to a single exon on the forward strand, but it is a *F region full of stop codons! *F Could it be an interesting RNA-coding gene \- No, is simply a spliced *F transcript for start of the next gene! *F These are some of these cDNAs, the AT ones are the new adult testes *F ESTs, with linkers attached! *F genomic, now forward caggaaggaacatttcagtattacaacatcaaccattctgaaattgttaaaa *F ttctaaaaggataaaaaaaatcatagtccaaattggaaattattcttgatatttcgtggatagaaagccgattgtga *F gccgttgaatagcgcgaacctattcaagacgagccaagcgatcgagttatcgcgaatatatataagatactaatact *F attggaggagaatttacgccgctcgacgattagacgggcgacgtgaatcgttttggagttttcaagaccttttgtaa *F tttgttttgttctctctaaagtatcacaaattgtgatatcatttagcacttttataatttctggaaaattcaagcaa *F cggattttgatctttgacctgtgcccttcgattgtaatacattcaaattgtaaagcgtgaagaaaacccacatattg *F acaaggatcagttcttttggaagcaccgaaaactaacgtctcaactaacgtcagaaacactcgcatgcaaaatgaat *F aagtttggtaagtcaatggggtttttactacaaaatcatatatttgaacattgtatatatggatggattgctttaaa *F attataagacacttaaattctagacattagactactcaagcaggttgtaaaagttattcgattttgtctcaaggcac *F ttatcactaattcagatatgtagatacataatacaaagtagactactcattggacggatgtttttgatatagtctgt *F tgtgttacaaatattcagtttaaggcacaatttacacattcgatttcttctcattgctttgtactgatctacaaata *F aattgcggaatgttcaggggggcaagacttccagaaacaaaaccaaaagcggacacggccagccactcgaacgtgtt *F ttagcagacgcgggcatttttccaaatggaaatggaggagttgcccgaatgctgagacagttactgcccaccgctgc *F tgccctgaaatgactatgaaaaatgtgtgaaaaagattttttctgcccctgtccagctacctatggctataaagttt *F taatgaaaaaagctggagatttctttttgccctggtagaagaccaagtggctgctaaactggttcctgcagcgcata *F gaaaaagttctcagggtacagataataaaaattcaagcgcatatgataatcaaggcgcaaaaaacaaaaagtggaaa *F aaacgccgcagcggcagcagcatcgacatacatatttaattcagcaaaaaaaatcgcagccaacagaccatcgacga *F tttaatataagaaaaatacgacggcaggcgttggattttttgtggcatccgttggtcggaaaaaaggtgtgtgtgcg *F ggcacaaacaaccctcagctaggacctggacgacctccccgatgggtgtaggtacgcctgggcttaactgggttccg *F atgttaacaggtttgcgatcgccgcacatacgcacacacagctgtgcgacttcacggacattagagaggaaggatct *F tccgaagaagaaaaatacgagctacacggcatttccgtaatctgagcgcagtaggcgcggctgtttgcgcttttctg *F acgatgctgctgcttttgcttcggctgctgctgctgctgcttggtcttctgccgcttttgatagaaatgacaaataa *F ccagattcattgtaatagattatgtctacaacttaatcgccttgcagat *F AT25734.5prime GGCACGAGG-CAGGAAGGAACATTTCAGTATTACAACATCAACCATTCTGAAATTGTTAAAA *F TTCTAAAAGGATAAAAAAAATCATAGTCCAAATTGGAAATTATTCTTGATATTTCGTGGATAGAAAGCCGATTGTGA *F GCCGTTGAATAGCGCGAACCTATTCAAGACGAGCCAAGCGATCGAGTTATCGCGAATATATATAAGATACTAATACT *F ATTGGAGGAGAATTTACGCCGCTCGACGATTAGACGGGCGACGTGAATCGTTTTGGAGTTTTCAAGACCTTTTGTAA *F TTTGTTTTGTTCTCTCTAAAGTATCACAAATTGTGATATCATTTAGCACTTTTATAATTTCTGGAAAATTCAAGCAA *F CGGATTTTGATCTTTGACCTGTGCCCTTCGATTGTAATACATTCAAATTGTAAAGCGTGAAGAAAACCCACATATTG *F ACAAGGATCAGTTCTTTTGGAAGCACCGAAAACTAACGTCTCAACTAACGTCGGAAACACTCGCATGCAAAATGAAT *F AAGTTTG------------------------TTTCCCACAACCTTGGTCGCGATCCATATCGGACCTTCAGCCCAGA *F AATGTACCCGTTATCAAGCCCATTGGGACCGCATGGAACTGAAATGGCGGAAGGTAATGGCGAACTGTTGGATGACA *F TTAACCAGAAAGCCGATGACCGTGGCGATGGCGAGCGTACAGAGGATTATCCCAAGCTGCTGGAATACGGTCTGGAC *F AAGAAGGTCGCCGGCAAACTGGATGAGATCTAC *F AT04521.5prime GGCACGAGG--------------------ATTACAACATCAACCATTCTGAAATTGTTAAAA *F TTCTAAAAGGATAAAAAAAATCATAGTCCAAATTGGAAATTATTCTTGATATTTCGTGGATAGAAAGCCGATTGTGA *F GCCGTTGAATAGCGCGAACCTATTCAAGACGAGCCAAGCGATCGAGTTATCGCGAATATATATAAGATACTAATACT *F ATTGGAGGAGAATTTACGCCGCTCGACGATTAGACGGGCGACGTGAATCGTTTTGGAGTTTTCAAGACCTTTTGTAA *F TTTGTTTTGTTCTCTCTAAAGTATCACAAATTGTGATATCATTTAGCACTTTTATAATTTCTGGAAAATTCAAGCAA *F CGGATTTTGATCTTTGACCTGTGCCCTTCGATTGTAATACATTCAAATTGTAAAGCGTGAAGAAAACCCACATATTG *F ACAAGGATCAGTTCTTTTGGAAGCACCGAAAACTAACGTCTCAACTAACGTCAGAAACACTCGCATGCAAAATGAAT *F AAGTTTGTTTCCCACAACCTTGGTCGCGATCCATATCGGACCTTCAGCCCAGAAATGTACCCGTTATCAAGCCCATT *F GGGACCGCATGGAACTGAAATGGCGGAAGGTAATGGCGAACTGTTGGATGACATTAACCAGAAAGCCGATGACCGTG *F GCGATGGCGAGCGTACAGAGGATTATCCCAAGCTGCTGGAATACGGTCTGGACAAG *F LP04990.5prime ATCAACCATTCTGAAATTGTTAAAA *F TTCTAAAAGGATAAAAAAAATCATAGTCCAAATTGGAAATTATTCTTGATATTTCGTGGATAGAAAGCCGATTGTGA *F GCCGTTGAATAGCGCGAACCTATTCAAGACGAGCCAAGCGATCGAGTTATCGCGAATATATATAAGATACTAATACT *F ATTGGAGGAGAATTTACGCCGCTCGACGATTAGACGGGCGACGTGAATCGTTTTGGAGTTTTCAAGACCTTTTGTAA *F TTTGTTTTGTTCTCTCTAAAGTATCACAAATTGTGATATCATTTAGCACTTTTATAATTTCTGGAAAATTCAAGCAA *F CGGATTTTGATCTTTGACCTGTGCCCTTCGATTGTAATACATTCAAATTGTAAAGCGTGAAGAAAACCCACATATTG *F ACAAGGATCAGTTCTTTTGGAAGCACCGAAAACTAACGTCTCAACTAACGTCAGAAACACTCGCATGCAAAATGAAT *F AAGTTTG *F GH18064.5prime CAACCATTCTGAAATTGTTAAAA *F TTCTAAAAGGATAAAAAAAATCATAGTCCAAATTGGAAATTATTCTTGATATTTCGTGGATAGAAAGCCGATTGTGA *F GCCGTTGAATAGCGCGAACCTATTCAAGACGAGCCAAGCGATCGAGTTATCGCGAATATATATAAGATACTAATACT *F ATTGGAGGAGAATTTACGCCGCTCGACGATTAGACGGGCGACGTGAATCGTTTTGGAGTTTTCAAGACCTTTTGTAA *F TTTGTTTTGTTCTCTCTAAAGTATCACAAATTGTGATATCATTTAGCACTTTTATAATTTCTGGAAAATTCAAGCAA *F CGGATTTTGATCTTTGACCTGTGCCCTTCGATTGTAATACATTCAAATTGTAAAGCGTGAAGAAAACCCACATATTG *F ACAAGGATCAGTTCTTTTGGAAGCACCGAAAACTAACGTCTCAACTAACGTCAGAAACACTCGCATGCAAAATGAAT *F AAGTTTGTTTCCCACAACCTTGGTCGCGATCCATATCGGACCTTCAGCCCAGAAATGTACCCGTTATCAAGCCCATT *F GGGACCGCATGGAACTGAAATGGCGGAAGGTAATGGCGAACTGTTGGATGACATTTACCAGAAAGCCGATGACCGT *F AT16285.5prime GGCACGAGG-----------------------------------ATTCTGAAATTGTTAAAA *F TTCTAAAAGGATAAAAAAAATCATAGTCCAAATTGGAAATTATTCTTGATATTTCGTGGATAGAAAGCCGATTGTGA *F GCCGTTGAATAGCGCGAACCTATTCAAGACGAGCCAAGCGATCGAGTTATCGCGAATATATATAAGATACTAATACT *F ATTGGAGGAGAATTTACGCCGCTCGACGATTAGACGGGCGACGTGAATCGTTTTGGAGTTTTCAAGACCTTTTGTAA *F TTTGTTTTGTTCTCTCTAAAGTATCACAAATTGTGATATCATTTAGCACTTTTATAATTTCTGGAAAATTCAAGCAA *F CGGATTTTGATCTTTGACCTGTGCCCTTCGATTGTAATACATTCAAATTGTAAAGCGTGAAGAAAACCCACATATTG *F ACAAGGATCAGTTCTTTTGGAAGCACCGAAAACTAACGTCTCAACTAACGTCAGAAACACTCGCATGCAAAATGAAT *F AAGTTTGTTTCCCACAACCTTGGTCGCGATCCATATCGGACCT *F So, figured it out, these start before our gene, and jump over it into *F the start of CG17838, so ours is in the first long 5'UTR intron of *F CG17838! *F Has tons of ESTs overlapping, so this annotation needs fixing. *F So I scan this 30kb region from our gene to CG17838 for anything else, *F beside one short exon in the middle that belongs to CG17838, and find *F some ESTs, but none coding. *F I can't believe there are not genes in here. There are several 500bp *F ORFs! See below for one! *F \***************extra2 GOOD GRIEF! in this 30kb region BLASTX reveals *F about in the middle of it a relative of the 300aa N-terminus of *F TGF-beta activated-kinase 1 homolog [Drosophila or CG18492 *F AE003732.2 aaataaaaattaatttgatttgcggggaagtcacaaagaataatattaagca *F tttttgataagttggaattgggtgaatgacggaattatttcttatcgcgtaccgataaggtggttttatctcactta *F actagcacttaatcacaactttcattgcaattcagttcacaaattgcacttcaaagcgatcgcacgtattttgctag *F agATGGTCAAGCAAGTGGATTTTGCGGAGGTGAAGCTCAGTGAGgtaggttttacttgaaatattgttaaggattca *F atgagacccccttttatgcttagAAATTTCTCGGAGCTGGATCTGGTGGAGCGGTGCGCAAAGCCACCTTTCAAAAT *F CAGGAGATTGCAGTAAAGATATTTGATTTCCTTGAGGAAACAATCAAAAAGAATGCAGAGAGGGAAATCACACATTT *F GTCGGAGATCGACCACGAAAACGTTATCAGGGTGATCGGGAGGGCCAGCAATGGAAAGAAGGACTACTTGTTGATGG *F AGTACCTGGAGGAGGGGTCCCTCCACAACTACCTCTATGGCGATGACAAGTGGGAGTACACCGTGGAGCAAGCGGTT *F CGCTGGGCACTCCAATGCGCCAAGgtaaagtgcaagatcgcctttccccacaatcagatacattttcggtgttttag *F GCCTTAGCATACTTGCATTCGTTGGATCGACCGATTGTTCACCGCGATATTAAGCCGCAAAACATGCTTTTATATAA *F TCAGCATGAAGACTTAAAGATTTGTGACTTTGGCCTGGCGACGGATATGTCCAATAATAAGACCGATATGCAAGGAA *F CATTGAGGTATATGGCTCCCGAGGCCATTAAGCACTTAAAGTATACGGCTAAGTGTGATGTGTACAGCTTTGGAATA *F ATGCTCTGGGAGCTGATGACACGTCAATTGCCATATAGTCACTTGGAAAACCCCAACAGCCAGTACGCCATTATGAA *F AGCTATCAGTTCAGgtaattattattatatacttattttaaatcataattccttaaatttacgttaattattataaa *F gaattattatattgtaagtatactccgtcccgatgaacttgagatctcggtacccaagagctgaaaggtgatatgca *F gattcctatgtcgtgcaagtttgtttgcgacgttcattataaaaatgtgtcaaaaaatattcgattttagaatagga *F atgatatttcttcaaatatagaaatatcaaattactaattttatttaaataaatttctgtttaatttcttttaaata *F tatatatatatacatatacatgaatacatatacatatgaatattttagGCGAAAAACTTCCAATGGAAGCAGTAAGA *F TCCGATTGCCCAGAGGGTATCAAGCAATTAATGGAATGTTGCATGGATATAAATCCCGAAAAGCGCCCCTCTATGAA *F GGAGATCGAAAAGTTCCTTGGCGAACAGTATGAATCCGGCACTGACGAGGACTTTATCAAGCCTTTGGATGAGGATA *F CCGTGGCTGTGGTGACCTACCATGTGGATTCGTCCGGCAGCAGGATAATGCGTGTTGATTTCTGGCGACATCAGTTG *F CCATCGATCCGCATGACTTTTCCGATAGTGAAACGGGAAGCCGAAAGATTGGGAAAGACCGTTGTCAGAGAAATGGC *F CAAGGCGGCGGCGGATGGAGATCGGGAAGTTCGGCGGGCTGAGAAGGACACGGAGCGTGAAACCTCGAGGGCTGCCC *F ACAATGGAGAGCGGGAAACGCGGAGAGCGGGTCAGGATGTGGGTCGTGAAACTGTACGGGCGGTCAAGAAAATAGGA *F AAGAAACTGCGCTTCTAACCAGAAATA *F translation *F M V K Q V D F A E V K L S E \------------------------------0----- *F \-------------------- K F L G A G S G G A V R K A T F Q N Q *F E I A V K I F D F L E E T I K K N A E R E I T H L S *F E I D H E N V I R V I G R A S N G K K D Y L L M E *F Y L E E G S L H N Y L Y G D D K W E Y T V E Q A V R *F W A L Q C A K \---------------------------------0------------------- A *F L A Y L H S L D R P I V H R D I K P Q N M L L Y N Q *F H E D L K I C D F G L A T D M S N N K T D M Q G T *F L R Y M A P E A I K H L K Y T A K C D V Y S F G I M *F L W E L M T R Q L P Y S H L E N P N S Q Y A I M K A *F I S S \----------1--------------------------1--------------------------1- *F \-------------------------1--------------------------1------------------------ *F \--1--------------------------1--------------------------1-------------------- *F \------1--------------------------1--------------------------1---------------- *F \----------1--------------------------1-------G E K L P M E A V R S *F D C P E G I K Q L M E C C M D I N P E K R P S M K E *F I E K F L G E Q Y E S G T D E D F I K P L D E D T *F V A V V T Y H V D S S G S R I M R V D F W R H Q L P *F S I R M T F P I V K R E A E R L G K T V V R E M A K *F A A A D G D R E V R R A E K D T E R E T S R A A H *F N G E R E T R R A G Q D V G R E T V R A V K K I G K *F K L R F \* *F So is a neat little kinase gene, with no ESTs, and only the N-terminal *F kinase domain having matches! *F So this is in the second intron of CG17838! Are there more genes in *F here or intron 1? I could do this endlessly, but without BLASTX or *F ESTs to guide it is useless. *F \***************A. mellifera BB260023B20H4.F AGTACGGGAGGACGTGCAAGGACATCGGTTGC *F ATGAGGGATGAGGTCTGCGTGATGGCCGAGGATCCTTGTTCGATCTACCAACGAGATAACTGCGGTCGTTATCCGAC *F TTGTATGAAATCTCGTCCAGGCGAGGCTAATTGTGCCAGCACTCTGTGCGGTGAAAACGAATACTGCAAAACCGAGA *F ATGGCGTCCCAACATGTGTGAAGAAATCAGCAGTAAATGATGACGGATTGTTCGCAGAGTTCGATGGAACAAGCAAC *F AGCTTATTGAGAAAAAGACGGGGGACCGGCGATGAAGCGGATTCCACATCGGATGACACGCAATCGGTTAAGACGAT *F GGACTCTCGATACCCATCCGGAAGTGGATATCCGTCCTCCGAAACTCGACCAAAAGCTGACACCTCGGTCAAATCAT *F CCGGTTACCCATCCAGTTCCGGTTATCCATCGAACTCTGGTTATCCCTCCACCGGCTCATCCGGTTATCCATCCAGT *F TCTGGTTACCCATCCAGATCGTCCGGATATCCTTCAGAATCGGCAAGTTCCGGTTACCCGTCGAGAAGCTCTGGCTA *F TCCTTCAGAGGCTGGATATCCGTCGAGAAGCTCGTCCGGTTATCCATCACAATCTGAATATCCTTCGAAAAGCTCTT *F CAGGTTATCCATCAGAGTCTGGCTACCCTTCGAGAAGCTCTTATCCATCAGGATCTAGTTATCCCAGTGGCTCATAT *F CCTTCATCAAGTTCAAGGGGATATCCATCGACCTCCGTCNATGAGGCAAGTGTGAAAAGGGTGGATGCAAACTCTTT *F AATGCTG *F long ORF encodes wacky protein with SSGRY repeats at end; weak BLASTX *F matchs to long Drosophila proteins; nothing better in nr; *F genomic match is weak, but to the least wacky part of the protein, and *F then there are many ESTs, and two good B.mori ESTs to this region, so I *F think it is a real protein; NEW INSECT GENE *F GH20482.5prime TGAAGGTGTCAGTTGGGCTCCCGACGGTTTAATTTTTAGCTCCCACAACGAA *F CGCAGTTCTCAGCGATTTTGACGCAAATTATAAACGTCAGCAACTTTTGATCCAAGGGCGTGACATCGATAGCGAGC *F AACAGGATGCTGGCACTGCCTTTGCTGACTCTGGCGGTCCTCGCCAGCTGCGGCTACTCCGTGGACGCCTACTCCAA *F GTACGGCCGTGGATGCGGGGACATTGGCTGCCTGCCCACCGAGGAGTGCGTCATCACCAGCGACTCGTGCAGCTACA *F ACCAGCGTGACGGCAAGGATTGCGGCAACTATCCCACCTGCAAACGGCGCTCCGGCGGAGGATCATCCGCCTCGAAC *F AGCAGCCCCAACTTGGCAGCCCCCTCGGCCAATCCGTCAGAGGTGAGCCATAACGCATACGCCCCGAATGCCCCAAG *F TGCCCCGAGTGCCCCGTTGCCGGAAGCGGATGCGAGCGGTGGTGCTGGCTACGGTGGTGCTGCGGGCGGTGGTGGAA *F GCGGCGGATATGGTG *F GH16618.5prime TGAAGGTGTCAGTTGGGCTCCCGACGGTTTAATTTTTAGCTCCCACAACGAA *F CGCAGTTCTCAGCGATTTTGACGCAAATTATAAACGTCAGCAACTTTTGATCCAAGGGCGTGACATCGATAGCGAGC *F AACAGGATGCTGGCACTGCCTTTGCTGACTCTGGCGGTCCTCGCCAGCTGCGGCTACTCCGTGGACGCCTACTCCAA *F GTACGGCCGTGGATGCGGGGACATTGGCTGCCTGCCCACCGAGGAGTGCGTCATCACCAGCGACTCGTGCAGCTACA *F ACCAGCGTGACGGCAAGGATTGCGGCAACTATCCCACCTGCAAACGGCGCTCCGGCGGAGGATCATCCGCCTCGAAC *F AGCAGCCCCAACTTGGCAGCCCCCTCGGCCAATCCGTCAGAGGTGAGCCATAACGCATACGCCCCGAATGCCCCAAG *F TGCCCCGAGTGCCCCGTTGCCGGAAGCGGATGCGAGCGGTGGTGCTGGCTACGGTGGTGCTGCGGGCGGTGGTGGAA *F GCGGCGGATATGGTGGTGGTTTCTCGGCTGGCGGCCACTCCCTGTACCCCAGCCTACCCAACTCAAACGGCGGCGGC *F GH10109.5prime AAGGTGTCAGTTGGGCTCCCGACGGTTTAATTTTTAGCTCCCACAACGAA *F CGCAGTTCTCAGCGATTTTGACGCAAATTATAAACGTCAGCAACTTTTGATCCAAGGGCGTGACATCGATAGCGAGC *F AACAGGATGCTGGCACTGCCTTTGCTGACTCTGGCGGTCCTCGCCAGCTGCGGCTACTCCGTGGACGCCTACTCCAA *F GTACGGCCGTGGATGCGGGGACATTGGCTGCCTGCCCACCGAGGAGTGCGTCATCACCAGCGACTCGTGCAGCTACA *F ACCAGCGTGACGGCAAGGATTGCGGCAACTATCCCACCTGCAAACGGCGCCCCGGCGGAGGATCATCCGCCTCGAAC *F AGCAGCCCCAACTTGGCAGCCCCCTCGGCCAATCCGTCAGAGGTGAGCCATAACGCATACGCCCCGAATGCCCCAAG *F TGCCCCGAGTGCCCCGTTGCCGGAAGCGGATGCGAGCGGTGGTGCTGGCTACGGTGGTGCTGCGGGCGGTGGTGGAA *F GCGGCGGATATGGTGGTGGTTTCTCGGCTGGCGGCCACTCCCTGTACCCCAGCCTACCCAACTCAAACGGCGGCTGC *F GGTGGTGCGGCTCCCTACAATCCATATGGCAATGGTGGCGGA *F This is outrageous! This transcript, and there are many more ESTs to *F confirm it, starts with 90bp 52994bp upstream in the next segment of *F the scaffold, jumps several genes, then has a series of exons with *F introns with more genes in them! I can't possibly put it all together! *F The C-terminus appears to be CG1726, however, so is not a new gene. *F fly *F MLALPLLTLAVLASCGYSVDAYSKYGRGCGDIGCLPTEECVITSDSCSYNQRDGKDCGNYPTCK-RRSGGGSSASNS *F SPNLAAPSANPSEVSHNAYAPNAPSAPSAPLPEADASGGAGYGGAAGGGGSGGYGGGFSAGGHSLYPSLPNSNGGG *F bee *F YGRTCKDIGCMRDEVCVMAEDPCSIYQRD--NCGRYPTCMKSRPGEANCASTLCGENEYCKTENGVPTCVKKSAVN *F DDGLFAEFDGTSNSLLRKRRGTGDEADSTSDDTQSVKTMDSRYPSGSGYPSSETRPKADTSVKSSGYPSSSGYPSNS *F GYPSTGSSGYPSSSGYPSRSSGYPSESASSGYPSRSSGYPSEAGYPSRSSSGYPSQSEYPSKSSSGYPSESGYPSRS *F SYPSGSSYPSGSYPSSSSRGYPSTSVMRQV *F So fly protein is a little longer at the N-terminus, that is, bee cDNA *F is truncated. Fly protein looks secreted. *F \***************A. mellifera BB270004B10G5.F GTCCCCCGCCGATTGATTTGGCGCGCGCGTGA *F CGGGAAAGAGGAACACGGGACTTTGGAGGTATTCCAAACGGCGGATATACACATCGTGGACGATGAAGTGGTGCTTA *F GTGATGCTGATTCTGGCCGGTGTCACGAGGGCCGACAATTCGGTCGACGCGGACTATTCGATCCTCAAGTGTCCGGA *F CCTGAATTCCCAAGAGGAGATCGATTTGAACGAGATAATGGGCAAGTGGTACGTGGTCGAGGTGTTGGAGCACAAAG *F TCGATCCATCGAAGCCCAACGGCTCGTACAAGGTCAATTCCTGCCCGATCGTCAAGCTGAGAGCGGTCGAGAACACG *F TCCAAGTACCTCTCCTCGTTGAGGCTGTTGTGGACCGAGGAGATCGGCGACCTCGAGTACACTTTCCGGATACCGGA *F CGTATCCAGGAAGCCGGGCTTTTGGATCTCCACCTCTGTGCAAAATGGCACACTGGTGGAGAGGGGGTACAAGCAAT *F TCAGCGGGAACGTGCACGTGATGAAGGCCGTCGCCTCGGACATGGTGCTGACATTCTGTTCCCGGAACCCGGACAAT *F CAGCTGTACTCGTTGCTACTCTCGCGGGAGCACATCTTGCAAAAGAGCGACAAGCGAGGGGTGCACAATCTGCTCGG *F CCGCCGCGGCCTCAAGATCGTCAATATCCGGG *F Long ORF encodes 200aa 13% leucine N-terminus; weak full-length match *F to insecticyanin A \- tobacco hornworm; BLASTX 22% over 197aa; p=3.2!; *F this is the genomic match, and one EST, and it is unannotated; NEW GENE. *F AE003553.2 TTCATCTGGAACAGACCGCATTCAGTGGCTCTTATCGGTAGAAACAGCAGCA *F CTTTTCCGAGATGTCTATCAAATCCTTGACATACGTTGCGATCTTTGGCCTTTTTTGGGGCTCAATTGCGGGAACTG *F TAGTTGATCAGTTTGGGATATATGGTGGTTCACCGATTACCACCACGGAAAGGAGTAATGCGGAGTTGCGCTGCATG *F AACATCAATCCGCAGAACTCGGTGGACTTGGAGCAGgtacgatatgataagataatatccttgagtgacaggaaata *F ttagctacacccaagatgaaaatgtaagctcatttgaatgcggagagctaatgcaattatggtcgatttgttctaat *F taaccctttgcgccctgagtgcgcccaatgtcaatgctgtacagatctgagccctgtttatttagttattttttttt *F ttcattgtgggcatttaattgaacgcacagcgagtggcatgcaaatagcacaaatgacccccattcgctcctacatg *F agtgtgtaataatgcccaacctctctgcatatatagATGATGGGACTCTGGTACGGCAGCGAGATTATCGTGCACAG *F CCAAGATTTTCCGGGCACCTACGAGTACGACTCATGTGTCATCATTCATCTGACCGATGCCACGGATCAGgtcagtt *F gccatcgaaattcaaaacatttagatactgattttaatcatatgaaatattacagATCCGTTTGAGCCAAGCAAATC *F GCGGCTATGGCTATGGAAATCAGGACTACAACCGTAACCAGAATAACTATGGACGCACCACCACCACTCAATCCTCC *F TATCCGGATAGCGATGAGTACCCGTTGAGATCGATTCAAAGCCAGCAGAAGTACCTACGTTTGATTTGGAGTGAGCG *F TGATAACAATCTGGAGTATACTTTCAACTATACCACCAGTGCACCTGGTCAGTGGTCCAACATCGGCGATCAGCGGG *F GATCCTTGGTCACCCTGAACACGTACACCCAGTTCACGGGCACTGTCCAGGTGGTGAAAGCGGTCAACGATCACCTG *F GTGCTGACCTTCTGCGGCAACGATGTTAAGAGCTCCATATACACAGTGGTTCTCACCCGCAATCGCCTTGGTCTCAG *F TTTAGATgtgagtaaagtagtcttctttcaatttcttttccagaaagttaaatctttgggtaattgtagtttgatta *F agtgtaaagaacactttttatttatctttaaatcagacctttttatttctcgtaaaatttgtttgtttcttaacaaa *F gcttaatatttgtttacaagctgcgtcaagtaataatatgcaaataatttttttagtaaatactgatagtgatgagc *F aaatctatatttgagaggtaaaaagaggttacattgtttacaattttacgtatatgctggtaacaaataagaatgag *F gattgtaggaatcgtatatgtatatattaaacctaattaatcattttttccaattttccagGAGCTGCGTAGCATCA *F GGAATCTGCTTTCCCGCCGTGGACTCTACACGGAGACCATTCGCAAGGTTTGCAATGGATGTGGGCGATTGGGTGGC *F AGCCTCTTCGCTCTTTTAGCCCTTTTGCTGGTCGTACGTTTGGCCTGGGGGCGTGGCCAGTGAGCTGGAGGGGATCG *F TCAGAGTGTCGAAGTACAGCCggcgtatttaatgagtccagccatattacgttaatttatgtataattttcccataa *F gcaatacacaagcgagatcgccgagtgctctccccgaaaactaactcacattgcctccgttttaattgctcgtcttg *F tcaattaaagtcaattacgaataaggcagggctgacttaagtgggcattagccgttggctagttgtaggcgttaagt *F gtttgcttaattcaattagcagggatctccacccgctcattggaatttcggtacaactaatgtcggaaaaaattcgg *F ttgatattgctgcaacgttcgcttcgtatggatctgcagaccccaaaagtaggcaacaaaaagtgtgctgctgaaat *F tgatacatataaattactcatacgccatggtgacacagtcacacacatcgaaacccaatgccacttgcctctgtctg *F attacgttgttaacatttcgtttttttttacactatcagcactaaccgaaaaacgagcagatgatc *F GH25183.5prime CTGGAACAGACCGCATTCAGTGGCTCTTATCGGTAGAAACAGCAGCA *F CTTTTCCGAGATGTCTATCAAATCCTTGACATACGTTGCGATCTTTGGCCTTTTTTGGGGCTCAATTGCGGGAACTG *F TAGTTGATCAGTTTGGGATATATGGTGGTTCACCGATTACCACCACGGAAAGGAGTAATGCGGAGTTGCGCTGCATG *F AACATCAATCCGCAGAACTCGGTGGACTTGGAGCAG---------------------0------------------- *F \---------------------------0----------------------------------------------0-- *F \--------------------------------------------0-------------------------------- *F \--------------0----------------------------------------------0--------------- *F \-------------------------------0----ATGATGGGACTCTGGTACGGCAGCGAGATTATCGTGCACAG *F CCAAGATTTTCCGGGCACCTACGAGTACGACTCATGTGTCATCATTCATCTGACCGATGCCACGGATCAG------- *F \---------------------------0---------------------------ATCCGTTTGAGCCAAGCAAATC *F GCGGCTATGGCTATGGAAATCAGGACTACAACCGTAACCAGAATAACTATGGACGCACCACCACCACTCAATCCTCC *F TATCCGGATAGCGATGAGTACCCGTTGAGATCGATTCAAAGCCAGCAGAAGTACCTACGTTTGATTTGGAGTGAGCG *F TGATAACAATCTGGAGTATACTTTCAACTATACCACCAGTGCACCTGGTCAGTGGTCCAACATCGGCGATCAGCGG *F translation *F M S I K S L T Y V A I F G L F W G S I A G T V V D Q *F F G I Y G G S P I T T T E R S N A E L R C M N I N P *F Q N S V D L E Q \---------------------0------------------------------ *F \----------------0----------------------------------------------0------------- *F \---------------------------------0------------------------------------------- *F \---0----------------------------------------------0-------------------------- *F \--------------------0---- M M G L W Y G S E I I V H S Q D F *F P G T Y E Y D S C V I I H L T D A T D Q \------------------ *F \----------------0--------------------------- I R L S Q A N R G Y G *F Y G N Q D Y N R N Q N N Y G R T T T T Q S S Y P D S *F D E Y P L R S I Q S Q Q K Y L R L I W S E R D N N *F L E Y T F N Y T T S A P G Q W S N I G D Q R G S L V *F T L N T Y T Q F T G T V Q V V K A V N D H L V L T F *F C G N D V K S S I Y T V V L T R N R L G L S L D \---- *F \--------------0------------------------------------0------------------------- *F \-----------0------------------------------------0---------------------------- *F \--------0------------------------------------0------------------------------- *F \-----0------------------------------------0---------------------------------- *F \--0------------------------------------0---------- E L R S I R N L L *F S R R G L Y T E T I R K V C N G C G R L G G S L F A *F L L A L L L V V R L A W G R G Q Z *F fly *F MSIKSLTYVAIFGLFWGSIAGTVVDQFGIYGGSPITTTERSNAELRCMNINPQNSVDLEQMMGLWYGSEIIVHSQDF *F PGTYEYDSCVIIHLTDATDQIRLSQANRGYGYGNQDYNRNQNNYGRTTTTQSSYPDSDEYPLRSIQSQQKYLRLIWS *F ERDNNLEYTFNYTTSAPGQWSNIGDQRGSLVTLNTYTQFTGTVQVVKAVNDHLVLTFCGNDVKSSIYTVVLTRNRLG *F LSLDELRSIRNLLSRRGLYTETIRKVCNGCGRLGGSLFALLALLLVVRLAWGRGQZ *F bee *F MKWCLVMLILAGVTRADNSVDADYSILKCPDLNSQEEIDLNEIMGKWYVVEVLEHKVDPSKPNGSYKVNSCPIVKLR *F AVENTSKYLSSLRLLWTEEIGDLEYTFRIPDVSRKPGFWISTSVQNGTLVERGYKQFSGNVHVMKAVASDMVLTFCS *F RNPDNQLYSLLLSREHILQKSDKRGVHNLLGRRGLKIVNIR *F So is a reasonably nice small gene, with several phase 0 introns. *F Protein at 250aa is a little long, and it doesn't match insecticyanin! *F Rapidly evolving insect proteins *F \***************A. mellifera BB270005A10H10.F GCAACGCTGGAAGATAATTTGCAAGTCGAAT *F CCAGACCAAGTTCCCAAGACGCGATTCAGTTTCTTCATCGGAGGACCACTATGCGGTGTCGTGGCATCGTCGAAGGC *F TCGCTCGTTCGACGAGGGGAACAGCGCTCGAGAGATTTCCGATAAGTTTGGCCGCGACGAGGATGGAGAGAATCCGC *F GGTCTGCCGGAACAGAGTTACGGAAATGCGCGCCGCCCCGGAACGCGACGACGACCTCGACGAGGCGACTGGCGTTC *F GTTTCGAGTTTCAAAGAGATCCCGGAGCGAGGTTCAAGTTCGCGTCGACCGGTGATTCGAGGATAAGGCACGGTTTG *F ATCGCGGCCCGCGTTGAAAATCGCTGAAAATCGTGAAGGATCGTGACCGATTGGATGGAAGATAGGGACGACGGGAC *F GATCGTGAAGAAGAGATGCCTGGTATCGTGGTATTCCGACGCCGATGGAGCGTCGGCAGTGACGACCTCGTCGTTCC *F CGGCGCTTTCCTCTTCATCCTTCATCTGATATGGATGACGGTATTGAGCGTTCTACTAGGGATATTTAAATGGGATT *F GCAATGTTATGTGCATCCTTCTACTGTGGAGATACATTGTCGGTTATTTGGTAATTTTCGTGATCTCCATGATCGTG *F GAGTTTTCCATCTGCTTTCTGGCCACTAGGGGTAGCATCCTGG *F unobvious internal ORF after long 5'UTR has e-05 match to N-terminus of *F a 680aa C. elegans predicted protein; this is the genomic match, and is *F to an unannotated region, but just before an annotated gene. *F No good ESTs, but some for same region to Brugia? Not sure about this *F one, would be hard to annotate. *F AE003493.2 ATGCCTGGACTTGTGGTCTTCAGACGTCGCTGGTCTGTGGGCTCTGATGATC *F TCGTGGTGCCGGGCGCATTTCTCCTGACGATTCATTTTATATGgtaagtagccacgtaccattttacttagtcatcg *F attattgttcataattctatttcgtgttttttttcttcttctttttcttcaacaacaaaataaaaacaaaaaaacga *F tgtgatttctatgacagttttgtgattgttagcgtctcgttggttatctttgagtataatacacgaattttaagcgt *F aaaattattgttctatcatctaataggctacttgttgatactattttgtaagtatactgaatcactttctgtggatt *F ctgtattgatattgatcctattttgcagtttcaatatgtgtagaaataggtatatgtgtgatctcgATGCGTGGCAG *F TATTCTGGATGCCGAGGCGCGCACCTCAATCAACATTTGGATATATCTCAAGAGCTGTAAGTTAGCACTAATAGAAT *F AATCCTATATGTATGTAGTATAC *F translation *F M P G L V V F R R R W S V G S D D L V V P G A F L L *F T I H F I W-------------------2------------------ *F bee MPGIVVFRRRWSVGSDDLVVPGAFLFILHLIWMTVLSVLLGIFKWDCNVMCILLLWRYIVGYLVIFVISMIV *F EFSICFLATRGSIL *F fly MPGLVVFRRRWSVGSDDLVVPGAFLLTIHFIW *F I can't really figure it out, but I think this is the first exon of *F gene CG11102 *F \***************A. mellifera BB270012B20H7.F ATGACACCAAAACCTAAGCAACAAAATATAAC *F GAATAAATCTAAAGAGAGATCCCCATCTATAGAAAAGCCAAAAGCGGAAGAAAAAGTAAAAATAACTAAAGTATTTG *F AATTTGCCGGTGAAGAAGTAAAAGTAGAAAAAGAAGTCTCTATAGATTCAGCAGAAGCAAGAATATCTCTATCCTCC *F GCTGAGAATTCTGAGAAAACAGGAAATTCTGGATCTCTCGCGGGTAGAGGATCTGGAAGAGGTAGAGGTTTCAAACG *F AGCTGGTTTAGGAGGTATTTCTTCTGTCCTTGGTCAATTAGGGAAGAAGGCGAAAATTAGTACGTTAGAAAAATCCA *F AACTAGATTGGGATAATTATAAGAAACAAGAGAATTTGGAGGAAGAAATTAGTACTCATAACAAAGGCAAGGATGGA *F TATTTAGAACGTCAAGATTTCTTACAAAGAGCAGATTTGCGACAATTTGAAATTGAAAAACAATTACGTAATGCAAA *F CAGACGTAGTACACGGTGAATTTATAATTTTATGTATATATATTTTATATATATATATCTTCCTTAATAATGAGAAC *F CAGAAATGGTATTGAGAAAAATATCTTATTAGAATTGCCAATTATTGGCGCTTGTAGCACATATTTACTCGAATTGT *F TTAAACTCTTACTAAATATCTCATGCCATGTATAAAAATGTGATTGCCTCGCTTGGCTTGACATCGGCTGG *F end of ORF encodes 14%K; 11%E 170aa; 50% match to end of 300aa *F craniofacial development protein 1 Mus musculus e-33; genomic match *F is to unannotated short scaffold; one Dros EST; NEW GENE *F AE003220.2 GACACAATGAACTCACAAAAAGAATACGTATCGGACTGCGAAACCGACGATG *F ATTATTATGTCGATTTGTTAACTTCAGGCAAGGGCAGTGATAAGAGTGAAAGTGATGTGTCGGACAAGTCTGAAAAT *F TATCCAGGCCTAAAATCAAAGCATACTGCGAAGGCATTGCGGAAAACAAGGCATTGTGACGGCGATAATAGGGAATA *F CAGGTCTAAGGAGTGCGACGACCTTCATTCCGAAGAGGAGTCTGAAAAATCGCGGTCGGATGCTTTATGGGCCGATT *F TTCTTGGCGACATTGATACTAAAAGCGTAATCAACCAAAAAACAGATTATACGGAGGGAAACGCAGCAAGTGCTACC *F AATACCAATACGCATGAGACTTGTAATAAATATGATAAAAACGATACGGCAATAATAAAAACTGCACAGCAATACGA *F TTCCAAAAGAACCACGCTTTCAGTTTCCACACTCGGAAAAATTAAACGATCATCCGCTGAAAAGAGTATCGGTACCA *F TGATAAATAAATTTGAAAAGAAGAAAAAATTGACAGTGCTTGAAAGGTCACAATTGGATTGGAAAATATTTAAACAA *F GACGAAGGCATAGACGAACTTCTGTGCTCGCATAACAAAGGCAAGGACGGgtgagtttggaagaagaagaagaagag *F tatttaaatggataaacttaaatttattacccaatgatttagGTATTTGGACCGTCAAGACTTTTTGGAGAGAACCG *F ATCTTAGGCAGTTTGAAATGGAAAAGAAGTTGCGGCTGTCTCGCAGGCCATACTAACGGCTTAACCAACG *F GH01620.5prime GACACAATGAACTCACAAAAAGAATACGTATCGGACTGCGAAACCGACGATG *F ATTATTATGTCGATTTGTTAACTTCAGGCAAGGGCAGTGATAAGAGTGAAAGTGATGTGTCGGACAAGTCTGAAAAT *F TATCCAGGCCTAAAATCAAAGCATACTGCGAAGGCATTGCGGAAAACAAGGCATTGTGACGGCGATAATAGGGAATA *F CAGGTCTAAGGAGTGCGACGACCTTCATTCCGAAGAGGAGTCTGAAAAATCGCGGTCGGATGCTTTATGGGCCGATT *F TTCTTGGCGACATTGATACTAAAAGCGTAATCAACCAAAAAACAGATTATACGGAGGGAAACGCAGCAAGTGCTACC *F AATACCAAT-CGCATGAGACTTGTAATAAATATGATAAAAACGATACGGCAATAATAAAAACTGCACAGCAATACGA *F TTCCAAAAGAACCACGCTTTCAGTTTCCACACTCGGAAAAATTAAACGATCATCCGCTGAAAAGAGTATCGGTACCA *F TGATAAATAAATTTGAAAAGAAGAAAAAATTGACAGTGCTTGAAAGGTCACAATTGGATTGGAAAATATTTAAACAA *F GACGAAGGCATAGACGAACTTCTGTGCTCGCATAACAAAGGCAAGGACGG--------------------------- *F \------------------------------------------GTATTTGGACCGTCAAG *F translation *F M N S Q K E Y V S D C E T D D D Y Y V D L L T S G K *F G S D K S E S D V S D K S E N Y P G L K S K H T A K *F A L R K T R H C D G D N R E Y R S K E C D D L H S *F E E E S E K S R S D A L W A D F L G D I D T K S V I *F N Q K T D Y T E G N A A S A T N T N T H E T C N K Y *F D K N D T A I I K T A Q Q Y D S K R T T L S V S T *F L G K I K R S S A E K S I G T M I N K F E K K K K L *F T V L E R S Q L D W K I F K Q D E G I D E L L C S H *F N K G K D G--------------------------------2-------------------------- *F \---------- Y L D R Q D F L E R T D L R Q F E M E K K L *F R L S R R P Y Z *F fly only 200aa *F MNSQKEYVSDCETDDDYYVDLLTSGKGSDKSESDVSDKSENYPGLKSKHTAKALRKTRHCDGDNREYRSKECDDLHS *F EEESEKSRSDALWADFLGDIDTKSVINQKTDYTEGNAASATNTNTHETCNKYDKNDTAIIKTAQQYDSKRTTLSVST *F LGKIKRSSAEKSIGTMINKFEKKKKLTVLERSQLDWKIFKQDEGIDELLCSHNKGKDGYLDRQDFLERTDLRQFEME *F KKLRLSRRPYZ *F bee *F MTPKPKQQNITNKSKERSPSIEKPKAEEKVKITKVFEFAGEEVKVEKEVSIDSAEARISLSSAENSEKTGNSGSLAG *F RGSGRGRGFKRAGLGGISSVLGQLGKKAKISTLEKSKLDWDNYKKQENLEEEISTHNKGKDGYLERQDFLQRADLRQ *F FEIEKQLRNANRRSTRZ *F There is about 15kb before this in this short contig; several EST *F matches, but only one is perfect, and then no ORFs, so seems is *F repetitive DNA. Indeed the sole perfect EST is for RT. *F \****************A. mellifera BB270013A20H11.F TTTTCCCGGTATGTGCTTTGCCTCGACAAG *F ATGTGCCACTATTGAACCAACAAAATCTTGGGAATTGACACCATTTTGTGGCCGTTCTACTTGCGTACCTGCTGATG *F ACAACTCTGGTCGACTTTTCGAACTTGTCGAAGACTGTGGACCACTTCCAAAAGCTAATCCGAAATGCAAACTCTCA *F GATAAAACTAATAAGACCGCTGCATTCCCTAATTGCTGTCCCATTTTCGAATGCGAAGAAGGAGCAAAACTTGAATA *F TCCAGAAATTCCAACTTTACCACCACCCACGGAAATTATAGAGACCGAGAAAACTTCAGAAGAAGTTCCGACAAAAG *F CTTAAATTCTAAAAAAACAGATTATAATCTTTACAAATTAAATTGAAAAATCGATTAAATTGAAACAGAAATTAAAG *F ATTTATTAATTATAATCTGAAATAATAAATTTAATTAAAAATATATATATATACTTCGTTAAAAAAAATATATTTTT *F ATCGAAAGTAAAAAAAAAATTTTACTTCTAACGAAAAATGTTATTTCATTCATTATATGTATACTGAAATATATAAA *F ATATATTTCTTATATTTATGCAATGATACAAATATAAAATTGCAAACTTACATTATATAAATAAATATATGCATACT *F AGTAAAATCATCAGAAACTTCGGGTATCCGTTCTAAAATATTGAATTTCTTCNATTTCCTAGTCCCGGAACC *F end of ORF encodes 13% proline; 11% glutamic acid; BLASTX match to *F Manduca sexta pMsmaD211! 77% and e-35; *F genomic match is similar and to unannotated region; ESTs from four *F insects! Clearly NEW INSECT-SPECIFIC YET REASONABLY CONSERVED GENE *F AE003844.2 RC CTTCATTTAGGCTGGTTAGGTGGTTAATTCCATTTGTCTTCGTTCTTTTGTA *F TTATTTTTACAAAGCGATAATATTTTAATCGTTTATGATTATTACAATATAACAAAAAGTTAACATCTTTGGAATCT *F TAAAAATGAGTTTTCATTTTGCTGTACTGACCCTTATTTTAACAGCCTTCACAGTTTCTCTGTGTGCTGAACAAAAA *F ATTACAAAGAGTGACGCAGGTGAAATACGAATTTTCAAACGTCTTATTCCTGCCGATGTTCTACGAGgtaagtatgg *F caatcatcagatttagaaattttccattattaaaagttacaagttcaatataagtatatctaaaacggcatgttgtt *F aaatcgggtgacacgcgtatagttttaagtaacataaaaggtatgggctagtgtaacgcaaaaaaaaaacaacaact *F aaatatccctctcctttctcaaggtattaattttggccacaaaaggtatcattcagcctatggtgaacatttatcga *F gtgtttttgcttttgatgtatacgtgatctattatatagtttccacagaaacagcccgaaaattaattggtctgtga *F gtgtattccaattattaacgtaggttcaatagtgtttcaaagctcgcgttttatctggccttgcggcttgaatattc *F cctcgcacttcctttcaaaacattttaataactcttcagATTTTCCGGGAATGTGCTTTGCTTCAACTCGATGTGCC *F ACTGTTGAGCCTGGAAAGTCGTGGGACCTTACTCCATTCTGCGGTCGATCTACTTGTGTTCAAAATGAGGAAAATGA *F TGCAAAgtaaacaaatttcagttaatatatatttaataaacaaatgcctaatatacattatttatagGCTATTCGAA *F CTCGTAGAAGACTGCGGCCCATTGCCACTGGCGAATGACAAATGTAAATTGGACACAGAGAAGACTAATAAAACCGC *F ATCGTTTCCTTATTGCTGCCCCATCTTTACATGTGACCCCGGTGTTAAATTGGAATACCCCGAGATCGGAAAGGATA *F ATGACAAAAAGAATTCTGAGTGATTCAAAACAAATATATTATGAAAACGTCTGTCAATACAATAAAAACATTTGTTG *F CTTTAGTCAAAAAGAACATTT *F LP07557.5prime CTTCATTTAGGCTGGTTAGGTGGTTAATTCCATTTGTCTTCGTTCTTTTGTA *F TTATTTTTACAAAGCGATAATATTTTAATCGTTTATGATTATTACAATATAACAAAAAGTTAACATCTTTGGAATCT *F TAAAAATGAGTTTTCATTTTGCTGTACTGACCCTTATTTTAACAGCCTTCACAGTTTCTCTGTGTGCTGAACAAAAA *F ATTACAAAGAGTGACGCAGGTGAAATACGAATTTTCAAACGTCTTATTCCTGCCGATGTTCTACGAG---------- *F \--------------1------------------------------------------1------------------- *F \-----------------------1------------------------------------------1---------- *F \--------------------------------1------------------------------------------1- *F \-----------------------------------------1----------------------------------- *F \-------1------------------------------------------1-------------------------- *F \----------------1----------------------ATTTTCCGGGAATGTGCTTTGCTTCAACTCGATGTGCC *F ACTGTTGAGCCTGGAAAGTCGTGGGACCTTACTCCATTCTGCGGTCGATCTACTTGTGTTCAAAATGAGGAAAATGA *F TGCAAA----------------------------------2--------------------------GCTATTCGAA *F CTCGTAGAAGACTGCGGCCCATTGCCACTGGCGAATGACAAATGTAAATTGGACACAGAGAAGACTAATAAAACCGC *F ATCGTTTCCTTATTGCTGCCCCATCTTTACATGTGACCCCGGTGTTAAATTGGAATACCCCGAGATCGGAAAGGATA *F ATGACAAAAAGAATTCTGAGTGATTCAAAACAAATATATTATGAAAACGTCTGTCAATACAATAAAAACATTT *F GH25016.5prime ATTCCATTTGTCTTCGTTCTTTTGTA *F TTATTTTTACAAAGCGATAATATTTTAATCGTTTATGATTATTACAATATAACAAAAAGTTAACATCTTTGGAATCT *F TAAAAATGAGTTTTCATTTTGCTGTACTGACCCTTATTTTAACAGCCTTCACAGTTTCTCTGTGTGCTGAACAAAAA *F ATTACAAAGAGTGACGCAGGTGAAATACGAATTTTCAAACGTCTTATTCCTGCCGATGTTCTACGAG---------- *F \--------------1------------------------------------------1------------------- *F \-----------------------1------------------------------------------1---------- *F \--------------------------------1------------------------------------------1- *F \-----------------------------------------1----------------------------------- *F \-------1------------------------------------------1-------------------------- *F \----------------1----------------------ATTTTCCGGGAATGTGCTTTGCTTCAACTCGATGTGCC *F ACTGTTGAGCCTGGAAAGTCGTGGGACCTTACTCCATTCTGCGGTCGATCTACTTGTGTTCAAAATGAGGAAAATGA *F TGCAAA----------------------------------2--------------------------GCTATTCGAA *F CTCGTAGAAGACTGCGGCCCATTGCCACTGGCGAATGACAAATGTAAATTGGACACAGAGAAGACTAATAAAACCGC *F ATCGTTTCCTTATTGGCTG *F translation *F M S F H F A V L T L I L T A F T V S *F L C A E Q K I T K S D A G E I R I F K R L I P A D V *F L R \------------------------1------------------------------------------1-- *F \----------------------------------------1------------------------------------ *F \------1------------------------------------------1--------------------------- *F \---------------1------------------------------------------1------------------ *F \------------------------1------------------------------------------1--------- *F \---------------------------------1----------------------D F P G M C F *F A S T R C A T V E P G K S W D L T P F C G R S T C V *F Q N E E N D A K----------------------------------2------------------- *F \------- L F E L V E D C G P L P L A N D K C K L D T E *F K T N K T A S F P Y C C P I F T C D P G V K L E Y P *F E I G K D N D K K N S E Z *F The first intron contains a set of NNNNNNNNNNs *F bee *F FPGMCFASTRCATIEPTKSWELTPFCGRSTCVPADDNSGRLFELVEDCGPLPKANPKC *F KL-SDKTNKTAAFPNCCPIFECEEGAKLEYPEIPTLPPPTEIIETEKTSEEVPTKA *F fly *F MSFHFAVLTLILTAFTVSLCAEQKITKSDAGEIRIFKRLIPADVLRDFPGMCFASTRCATVEPGKSWDLTPFCGRST *F CVQNEENDAKLFELVEDCGPLPLANDKCKLDTEKTNKTASFPYCCPIFTCDPGVKLEYPEIGKDNDKKNSEZ *F \************There is 20kb to the next gene, YIKES, each 10kb half *F contains at least one huge gene, one ORF is 5kb, the other is 7kb! *F Amazingly there are no ESTs, and the few BLASTX matches are poor *F BUT amazingly the 7kb one is to the entire TES domain of lacunin, but *F with nothing else, that is, no Kunitz or thrombospondins? What on *F earth is it? *F SIMA \- these have got to be genes, I can't see how one could have *F several kb of ORF without it encoding something selected. *F The latter TES region is a threonin/glutamic acid/serine rich region of *F a moth protein we described, but this is not it's ortholog in the fly *F genome, that is elsewhere and already annotated. *F \**************A. mellifera BB270014A20B4.F TATTCTACAGGTTCCACAGCACCGTTTGCTTGT *F GCGAGGTCTGGAAAAGAGCGTACAAGGTGAAGCCTACATACATGTGACGTAAGGGGTCGCGTGAGGTCGTGTGCAAG *F ACGGCGGCTGCTCCTCAACGACCGAAAACCCTGCCGGCCAGGCTGGACGCCATCGCGGAAAACGATCCGATTTACTT *F TATGTAAGAGGGAGAGGGAGAGATGCGAGATTCTCTTTCGCCTCTCTTCGTCGCTCGAGGTTCTTCAAGCTCTGCGA *F AACTGCGAATATATATATATATATATATATATATCGAGTATATCGTTCACCAGGCGTTGTTCCATAGATATTGATTC *F GTGCGACGAGCGATGGACGAGCCTCGAGTTTGAGAAGGAATGGCGGTTTGTTTATTATTATTCTTTTTTCTCGAGAA *F GTTGCGTTTATTTATATTATTTAGATAATANNAATGATTGTACAAGGTTNTATAGTCGTCCTCGCGGTCTATGGAGA *F GAAGAANAGATCCCCATGCGCGATGGTTTTTACATACACACTACCATACATACACCACACGGCGATT *F The match is barely the end of an ORF, which encodes the end of a *F family of proteins. The best genomic match is not properly annotated, *F but there is a gene in the region; there are many related lower matches *F in Drosophila and C. elegans, e.g. CG3332, and there is an EST for this *F one. *F Leave it for now *F \**************A. mellifera BB270014B20D9.F ATAATTTCTTCTTCTTCTCCATTTCCATTTCTT *F CGATGTCGAGGGTGTGATGCCGTGCGAGCCAGTGTCCGCGAGCGACTTTGTCGAATTTTCGAATTTCATGTCGAGGG *F ACCGGAAGTACTCGAGGCACTGCGGCCAACAGAAGGAGTTCGACGTGAACAGCGACAGAAAGTTCTTTCGCGTGACG *F TTCAAGAGCAACGATAGATACGACGGGACAGGATTCAACGCTAGCTACGTGTTCGTGGATGACGAGGGAAATTACAC *F GACGAAGCCGCCGACGAGTAACGCGTCAACGTTAAAAGGTGCAACGATGATGATGCTGCTGCTGCTGCTCGTGTTCA *F CGGATCCTCTTCTCCTCCGTTCCGGCCGAGTTTCACCACGCTTTAATCACGATCAGTAAGTTGGACGGGATGGTCTT *F GGCTCAATTTACAACGAACTATAACTTGGAAGACGGGGAGCCATCCGAAATCTTCCATTATACTCGGCTAGCCGAGG *F AAAGTGGCCGCATCGTTTGCATCGATGTGAAAATCGGGGGGACACATCTTCGAGGGACGCGTTCGTGATTCGTTTAT *F TATTATCATCGTTATCACGGGAACCTTGTCTCCAACCAAACGTTATTATTATTATTATTATCATCTCGTTCACGTTT *F CGTAGTCGTTGGTTGGGCGACAGAAGAACGACAAATATATATATATATATATATATAAATATAAATACTAT *F no obvious ORFs; BLASTX match to C. elegans C15A11.3 37% over 74aa; *F 7e-09; genomic match is same region at 50% and e-19; needs to be added *F on to CG4940? no EST unfortunately *F \**************A. mellifera BB270018B10D12.F GTAAGCCAACTTCATCGACCTTAAGCGGATTA *F CGACGCTGTCGAGCATTGTATGATTGCGAACAGATAACGAGGATGACTATCATTCCGGGAAGGAGAGGGATCTCGTA *F ACAAATGAACAAACCGACGACGACAACGGATGGAAGGCGCTCTTGAACGAGCGCCTGAAAGAAGAGGAATGTTCCCG *F ATTAGTTTCGTGCACATGTTGCAAGATTAACATTCGGTAAGCCTGAACTCATTGGCAAGTGTCTCCAGTACTGCCAG *F CTCCGTGAGCATTGCTAGTCTCGGCAGGAAATCTTGGACGAGGAAACCGAAAGATTGAACAGAAAGGATCAAGCATT *F CAGTTCCATAATTTTTAAAAAGTCGTGGATTCCTTCTCTTGAAATTATACGCAATTTGAAATAATTCCTCAATTTAT *F AACTTGAAACAATTCCAGAGAAGCACATTGATTTGCTAAAAGAAGAGATTTTAATAATTATTATATGAGAAAATACT *F AAAAAATATTTGTTTAAATTTGAGAGGAGGAAATCATAAATATTGAAGAATTCATTAAAAAAAAAAATAATATCTGA *F CTAAAAAAAATCAATATGAAAGTTGAGAAAGTTTGTCTTTGAGAATCATTAGAAACATCATAGAGAAGAGAAGTAGT *F TACTATAGCCTGAGCAAAATAAATTGGCTATTACAAGTTACAAATAGATAAACTCTCATCAT *F no obvious ORFs; short stretch matches end to ADP-ribosylation *F factor-directed GTPase activating protein in mammals; could be *F C-terminus of CG2226?; there is one Droso EST! *F Indeed this region is included in the annotation, but not the protein. *F SIMA \- This seems to be a common problem I don't understand, why are *F the annotated mRNAs not always completely translated? *F \*************A. mellifera BB270019A20H6.F TTTCCACTCGAATTTTTCTACGCTCGTGTACGGT *F CTTTTCACTCTCTCTCTCTATTTCTCTCTTTCTCTCTGTTTCTCCCCTTAATCGAGAGAAGTTGAAGTAGCGGACAG *F AACGTTTTTTTTATGGAATTTCCAGTTAAAAAATAATCGTTTTCAAACTCACCTCGTTGGTGTTCCATCGGTGCCTC *F TGAGTTGGAAAATGTTCAGCTCGTGGCAAGGTCTCCAAATTCTCCGGAAGTTTTATCGGATCTCCATCTAAACGGGG *F AGGAAAGAAAAAAAAGAATTCGATGAAAATCTGCTCTCGACCAATTCGAACGTTTTTCTTTCTTTCTTTTTCTTTTT *F TTTTCTCTCTTTTTCGAAACGCGTAAAATATACGAGTTTTTCGAAAAATTCAAGGAAATTTAATATCCTAATGGTCG *F AATCAATTATATCATTCAATTCGATTAACTCTTTCTCAATCGTTAATCCGATTATCGTGCGAGTTACTAATAATTTG *F CGCTCACATATATTCACTTCGACAAAAGCTTAGATGTTTGCATAACACATCATTAATCGTGTTTATATGCAACGGAT *F CGAAGTATGCTATCGATCGTAACACAACTCGTGTACGAGAGAAAGAAACAGTGGTGTTAAAAGTAATGCACTTTTCA *F CGTGCATAATATTACTTTCCCGGGATATGANACGCATATTTACTGTNNTAGAGAGNAAGNAAAGAAGA *F no obvious ORFs; no BLASTX hits?; genomic hit is from a short internal *F region of RC, to unannotated region; no Dros ESTs, but a few others for *F same region, could be a real protein? *F \*************A. mellifera BB270020A20G6.F AATACTTCTGTTGTTAAAGGTGTTGAAAGTATAT *F TATCAATTAAGTTTGATCATCCTCTTCTAAAAGAATTAGTCATTGTAGAGGAACCTACATCAACCCAGGAGCCAATT *F GTTTCCAATGCGGCAGTAGTCTCTGAATGTTATAAAGTGACTGCCGATGTTTTACCAGTACTATCAAAATTTGGATA *F TGAAAAAGGAGACATTATGAAAAGAGCTGAGATCAGAAAATGTTTTACTGAATACGTGAAAGCAGAGAATCTTCAAG *F ATGGAAGGATACTGAAACTGAACCCGCAACTCGCAGGTATTATGAAAACTAAAGCGAATGTGGAAACTGTAATGATG *F GAGGATGGAATAAACAAGTTTATTGGACGTATGACGCATATGCATGAAGTTACTTTAGCAGGAAATAAATTGTTACA *F CACGGGTAAATTGGAACCTATTGATATGAGAGTCACTGTTCGATCCGGCGGCAAAAAGGTAACGCTAGTAAATAATT *F TGGAAACATTTGGCATAAATGCTAAAGAATTTAGTAAAGAATGTCAGAATATTGGAGCGAGTGCAACAATTACGGAT *F GAACCAGGAAAAAAAACTCCTAGTGTTCTAGTTCAAGGAAATCAAATTTTATATATCTACAAATTACTTACAGAAAA *F ATATCANATTAAAAAAAACTATATAAGAGGATTAGAATTCGCTCCAAAGAAACAAGGTTC *F long ORF encodes 220aa 11% lysine end of protein; indeed is end of *F ligatin Drosophila melanogaster by BLASTXl e-18; not in Drosophila *F genome set for some reason; *F curiously vertebrate proteins are same or better identity, and C. *F elegans! Appears not to be annotated properly. *F \************A. mellifera BB270021B10C10.F ACAATTTCTATTTAAGAAAAAAAATCTAAAAACA *F TGAAATTTAGATTTTTAGGTGATGGCGATTGCCCTGATTGGTTNGCTAGCCGAAATCAACACATTGTCACGTATGAC *F ATCCATTAAAATTAAGATATTAGGACAAACGGTTGCAAAATATCTTACGGAAGGAGAACTCGATGAAGAAAAAATAA *F AAAAAATTACTCAAGATGCCAAGATTGAACTTAACGATGCAAAGGCTATGGTAGCAGCTCTTGAATTAATCTTTACA *F TCGTCTGCTCGATATGGCGTTTCCGCCGCCGATTTAAGCAATGAATTGCAGCAACTAGGACTCCCTCGTGAGCACAG *F CGCTGCAATTGCCAGATTGCATACGGATTATTGTCCTCAAATTACTGCTACGCTGTCTTCCCAATCCTTGAGAGTAA *F GCAGATTATCGTCGATTGAAGTTTTGTCCTGTGATAATTCGTCACCTTTCTCCACGGTATCTCTTAAATTAAAGAAA *F TTGGATGGAAATGTGGAAGATTCTATTATTAATATTTCAAAAAAAGATGTACACGTTCTATTGGCAGAATTACGAAG *F AGCCAAGTCATTGATGGAAAACCTTTGAATAAAATAATGTTCTACGTATTAAACACAATTATTTTTATTAAAATAAT *F AAAGTATATTAAAGTTATTATCTACAATAATAAAGTACTGGCTAAAAAAAAAAGTAAAATATAAAAAAAAAAA *F long ORF encodes 180aa 14% leucine 12% serine protein; 48% match over *F full-length to similar hypothetical protein FLJ20452 Homo sapiens; *F e-21; also similar C. elegans protein; *F unannotated NEW GENE; no Drosophila ESTs, but tons of others! Strange *F AE003534.2 taccgacatttggtttgccctttacagAAATTCCGCTTCTGTGGCGAAGGCG *F ATTGCCCCGATTGGGTCCTAGCTGAGATCATATCAACACTCTCGAACTTGAGCATTGAAAACTTGGAACAACTTAGC *F GATTTAGTGGCACAACGAATTTGTGGAGAGACATTTGAGgtttgtaattatttgtttgaaattcataaatatacaag *F acttttactttcagGAAGCGAAAATAAAATCGCTGACATCCACATTAACTAATGAAGGAAAAACCGCCGTGGCATGC *F ATCAATTTTATGCTGACCAGCGCAGCTCGCTATAGCTGTAGTGAAAGCATTTTTGGCGAGGAGATCCAGCAATTGGG *F ACTTCCCAAGGACCATGCCGCAGCCATGTGCAGAGTCCTCCAAAAGCATTCCGCCACCATAAGGCAAACACTTATAA *F ACAAATCTTTCAGAAgttagtggtctaaacacatattaagtcttatgtgctatcttattaaggcttatatttgcaga *F ttaatttgcttcaattttatatttattttagTTAACGAACTGACAAGCGTCCGAGACATATCTACGCCAGGGCAAAC *F GCCTCCAAACTACGCCACCTTGGAACTGAAGATCTCGCAAGAACTGGTCGATGGCCTACCGAAGGATACCACCCATG *F TCCTCAACATTGATCGCACCCAAATGAAGGCTCTGCTGGCGGAGCTGAAATTGGCACGTGATGTTATGCAAAAATAT *F GAAAATAAACCAGATTCCTAAAAATGTTATTAATA *F translation *F K F R F C G E G D C P D W V L A E I I S T L S N L S *F I E N L E Q L S D L V A Q R I C G E T F E \-------------- *F \-----------0-------------------------- E A K I K S L T S T L T N *F E G K T A V A C I N F M L T S A A R Y S C S E S I F *F G E E I Q Q L G L P K D H A A A M C R V L Q K H S *F A T I R Q T L I N K S F R \-------------------------------------- *F \--------1----------------------------------------------I N E L T S V R *F D I S T P G Q T P P N Y A T L E L K I S Q E L V D *F G L P K D T T H V L N I D R T Q M K A L L A E L K L *F A R D V M Q K Y E N K P D S Z *F Can't easily find the correct N-terminus for this, but anticipate that *F it will be short. Need an EST! *F bee *F MKFRFLGDGDCPDWLLAEINTLSRMTSIKIKILGQTVAKYLTEGELDEEKIKKITQDAKIELNDAKAMVAALELIFT *F SSARYGVSAADLSNELQQLGLPREH------SAAIARLHTDYCPQITATLSSQSLRVSRLSSIEVLSCDNSSPFSTV *F SLKLKKLDGNVEDSIINISKKDVHVLLAELRRAKSLMEN *F fly *F KFRFCGEGDCPDWVLAEIISTLSNLSIENLEQLSDLVAQRICGETFEE--AKIKSLTSTLTNEGKTAVACINFMLTS *F AARYSCSESIFGEEIQQLGLPKDHAAAMCRVLQKHSATIRQTLINKSFRINELTSVRDISTPGQTPPNYATLELKIS *F QELVDGLPKDTTHVLNIDRTQMKALLAELKLARDVMQKYENKPDSZ *F \************A. mellifera BB270025A20A3.F AGTTCTAGATCTTGCCACTGAAACTGCTACTGCTG *F TAAGAGAAACAAGTAGAAGTGCTCATCGTACGATACCAAAACGCGATAGACCTCCTCGTGTGGCAAGTGGTTCTGCT *F GGTCTATTACCACCCTATAATCGCCAACAAGCAGAGGGCCAAGAATTTCTTTATATAATAAATGAACATAATTATTC *F AGAATTATTTGTGGCATATGAGTGTTTACGTAGTGGAACGGAGAATCTAAGAATTCTTGTTTCTAATGAAAGAGTTC *F GAGTGATTTCCGGAGGTACCAAAGGAGTTGTAACCGAAGTCAGTCTAGCGGACTTATTATATTGTCAACCAATGCAT *F AAGCTAGAAAGTAATGGTGTTACTTTATACTATATTGAATTAATATCTAGATCAGATTCAACGATAACCGTTAACAT *F GGACGGTCCAGAACTTCTAAGAAGACCTAAAGTTCGATGTGACAATGAAGAAGTAGCCAAAAGAGTATCGCAGCAAA *F TTAATTACGCTAAAGGAATGCACGAGGAACGTAGCTTGACTCTTTCTTCTTCGGATAATATGTTAGATGATGTACAG *F TACTATAAGTAGTTACAAACAATCATATATGAAAATTTATTTTGTATTTGACAAAAGTTTGGAATCACTATGTTTTT *F ACAAAAAATTTTTATGGGAAATTAATGCATTAAAATATTTTCATTTCAATGTTAATTCC *F long ORF encodes normal protein; several weak matchs to Drosophila *F proteins, none convincing; but also several human proteins, especially *F KIAA0453 protein Homo sapiens at e-19; *F seems to be a missed exon of CG11003! Which is also one of the weak *F matches above for part of it. No ESTs to help, but I think it is part *F of CG11003. *F \**************A. mellifera BB270028A10H8.F GCCGTGGCCCGAATTTTATCAAGAACACAATGT *F CGGAAGATCAAGTAAATCCACCATCTCCAATCGATGGTATTTTACCGTTTTTGCAAAGTATTGAATGGAGAGATCCA *F TGGCTTGCATTATTATTAACTTTTCACATTGCTGTTACTTTGACTGCATTGATGACACGAAACCATGCCAATTTTCA *F AATTATGTTATTTCTTGCACTATTACCTCTGGTATATTTTTCTGAAAGTATTAATGAAGTTGCTGCATCTAATTGGA *F TGTTGTTCTCAAGACAGCAATATTTTGATTCCAATGGTCTCTTTATATCTGTAGTATTCTCTGTGCCTATCTTGATG *F AATTGTATGATCATGATTGCCAGTTGGCTTTATCAGTCTAGTCAATTAATGACCAGTTTGAAAAGAGCGCAATTAAG *F ACAACAAGCAAGAAATCAGGAAATAGGAAATGAATCAATAAATACAAATGGCACTGCTGCAAGAGAAAAACAGGAGT *F AATATTTCTAGTCCAAGAACAATGAGAAATGGAAAATACTCTATAAGTAGAGTCGTATATAACGGCATTGTAAAATT *F CGACGAATATTTTCAACATAGTATTTTTTTAAAAGATTACTGCCGACACTTGTTATCACTGTACTTCAAGTTGATTA *F ATTTCACTGTCAGTTAACTATATTTCCAACTTTATGCCGTATATACATATATATCGACTATTAGAA *F end of ORF; weak long match to OstStt3 gene product; 23% and e=1.3; but *F 48% and e-23 to end of 171aa hypothetical protein DKFZp434C1714.1 \- *F human (fragment); *F genomic match is unannotated short region, NEW GENE. Tons of ESTs *F from cow, plants and others, but not Drosophila! *F This is the entire available region, assuming flanking annotations are *F correct. *F AE003822.2 gaaactcccgccacaagcgctttagaacagagtcctagacgagtgtggtgca *F cggtagggtcggtggcccgtgccacatctaaggcgccccttttttcggattaccctgctcggctttagcttcgattg *F cttgctcacacgtcgcccgttcgacttaataacccgaatagatttgattcgccctaaaaactacaattttgactgtt *F ttaaaacgaattctttgtgatatttttcggatttgttaatgttgtctactgagtcagtgaaagcgttatcgacacgt *F tcagactgaatgacgggcagggcgactctgcacgacaagtcggggtgggtgagaatgaggtggcacaaaatttgtaa *F ttgcatttatatggtgagtaatacatactaaacgaaataatagtattttgatttatgttgttatatttagcccataa *F aatagtaagttaggtcttacaaacagcgctaccagatccagtcaaaattgaggaagcctgaacgtctataggcctcc *F caaaatggcgttgccATGAAAAATGACAGCTGTTCGCTGCGAATGGCTATTTATGTTTGTTTTGACTCGGCTTTCGA *F ATATATCGCAAAATATATACAGGAAACATTTATATTCACAAAAATCTGTACGATGCACCCAGGGCAAATTGAGGTCA *F ACGAGATCAATGGCTATTGGACATTTCTGCTGAGCgtaagatactcgcctatatacaatcaaaaatcaagaatccgg *F caagttgtcactatttttgcagATCGATTGGAAGGATCCCTGGCTTATTGGCCTTATTTTGGCGCATATCTTAACCA *F CCACCACTGCGCTGCTCAGCCGGAACAGCTCCAACTTCCAGGTTTTCCTCTTCCTAGTACTGTgtacgtggacttgg *F cggcttccttgacttacccgataaatgactctgatttttgcatgtgcttcatcttcctcagTGCTGGCAGTCTACTT *F CACCGAAAGCATCAATGAGTTCGCTGCTAACAACTGGAGTTCCTTTTCCAGACAACAATACTTCGATAGCAACGGCC *F TGTTTATCTCGACAGTTTTCTCAATACCTATTTTGCTTAATTGCATGCTTTTGATTgtaagttatagtgtttccact *F gcatgaagtgtgtatttatctttgcttatttgcagGGCACTTGGCTCTACAACTCCACGCAGCTGATGGTGACTCTA *F AAAACAGCGCAGCTCAAGGAGCGAGCTCGCAAGGAACGCCAGACTAAGGCGGATTCGGAATCCATAGCACATAAAAA *F GGCAGAGTAGaacttacgcctgtattacatgcagttaaaagcacaagtagagctgtgaaattatatgttatgcttta *F aatggattttcctgtcatctagatgtagtttgctgcacagctctcgtctttaaaataaatttaatttagtataatca *F aacttatagaatttgtaaatttaggctatttttacatcctgttttacttagcgaagttacaaacctaacatgccctt *F catattaagcaaaaaatcacaccagttaccgttgccaccttggtaaagcagtttttactgccacctaaaattttcta *F tatatatcacgtaatatgaactattttgatatttttgacgaaattaacattatagatccaatcagcttattgcctgt *F atcaatttctgatctgtgtgccaagactgtaatttcaaattagaagctcgttggacctgtgtcattttttagtacga *F attcaattgggagcccttcgtcgtctggtaacactgtccaacgattttgttgttgctggcttgtgggtgtcgaagca *F gtgtcgcggcgcaatgttggaagtggtttttgggtaa *F translation *F M K N D S C S L *F R M A I Y V C F D S A F E Y I A K Y I Q E T F I F T *F K I C T M H P G Q I E V N E I N G Y W T F L L S \----- *F \-------------------------0--------------------------------- I D W K D P *F W L I G L I L A H I L T T T T A L L S R N S S N F Q *F V F L F L V L \-----------------------------------1------------------ *F \---------------------L L A V Y F T E S I N E F A A N N W S *F S F S R Q Q Y F D S N G L F I S T V F S I P I L L N *F C M L L I \----------------------------------0--------------------- G T *F W L Y N S T Q L M V T L K T A Q L K E R A R K E R *F Q T K A D S E S I A H K K A E \* *F This is my best guess, because two intron boundaries are unpredicted. *F fly *F MKNDSCSLRMAIYVCFDSAFEYIAKYIQETFIFTKICTMHPGQIEVNEINGYWTFLLSIDWKDPWLIGLILAHI *F LTTTTALLSRNSSNFQVFLFLVLLLAVYFTESINEFAANNWSSFSRQQYFDSNGLFISTVFSIPILLNCMLLIGTWL *F YNSTQLMVTLKTAQLKERARKERQTKADSESIAHKKAE *F bee *F RGPNFIKNTMSEDQVNPPSPIDGILPFLQSIEWRDPWLALLLTFHIA *F VTLTALMTRNHANFQIMLFLALLPLVYFSESINEVAASNWMLFSRQQYFDSNGLFISVVFSVPILMNCMIMIASWLY *F QSSQLMTSLKRAQLRQQARNQEIGNESINTNGTAAREKQE *F Looks good from the alignment though. *F \***************A. mellifera BB270030B20G7.F ACTATTCTCACCTCCGGCCGATTTCACGCCGC *F GTAATTCTCATTTCTTTCGACAATCGAATATCCGTCGATCACAGTGATTATTATTTACGACTTGCTGGAATAACAAT *F CACGCGATTAATTTGTTAAGTTTCAGTATGGAGTGTCCTGAAGCGATGGAACGAGGCAGAAACTTTCGTTTGCTTGC *F CAAGGAAGAACTACCTAAACTCTTGGACTTCCTTGATGGCTATTTGCCCGAATCCTTAAAGTTCCATCAAACTTTGT *F TGACCTATATGAATGACAGGGTATGGGATTTTATTTTCTATGTGGCTAATGACTGGCCGGATGATGAGATCTGTTTA *F CATTTTCCAGGCATGACGTTAGCCACATAGAAAAAAAAAGCAAC *F possible internal ORF; weak match to CG5750; no better BLASTX matches; *F but convincing genomic match for same region at 70%, to unannotated *F region, but could be real N-terminus of CG15628? No ESTs to help *F \*************A. mellifera BB270032B20A6.F GGAAGGGTGCGTGTCAAAGTAGTAGACACACAAC *F TGCTAATCTCGTGGTTACATTTTATTTTCACGAATATCTTAGGAAATGTACTTTTTCGGCACATTGCTATGTAGCAC *F GTAAATGAAGCGACGGCGTATAGCGCGGTCGCATATCAAAAGAATACTACCTATAGGAACGATGAGAATGGCGCGCA *F AATGTTGCGTGCGTAGCTGTGAGGCTGATGTGCAAGATGCGCGTGCTAAAGGGTTACCGCTTCATAAATTTCCGAAA *F GATATTACTTTAAGAAACAAATGGTTGACTAGTGGTGGATTTGACGCGAATTTTAAACCTTCACCAGGTCAAGTTGT *F TTGTCACAGACATTTTAAACGAGCTGATTACGAAGCTGCTAAAGGACATAAATTACTTCTACGTAAAGGTAGTGTTC *F CGTCGGTTTTTGCAGATTATGACAATCATCCGGATCCTGTAATAATGTCTGTAAAATCATCAACTTCTTATGCACAA *F GAAGATTTAGATCTTATTAATTCTGAAATTTTGAATTTAGAACAATCCATATCTCCATTGAATTCTGGTGCCAGAAC *F ACCAAAATCCGATAGCTGTGGAGAAACATGTTCTTCTCGACCAGAATCATCAGCTGATTCTTTTAATTTATTAGATT *F CAACAGAATTAATTGATANATGGATGTAAAACTTTGAATATGAAAGAAGAGAATATATCTCCTATGA *F long ORF encodes 200aa 12% serine protein; repeated weak matches to *F huge protein CG10631; e-05; also weak match to dJ126A5.2.2 (novel *F protein) (isoform 2) Homo sapiens; short protein at e-04; *F genomic match seems to be to region of N-terminus of CG10042 gene *F product alt 1; several ESTs for this match too, so could be new gene, *F but hard to annotate. *F \----------------------------------------------------------------------------- *F \-- *F 'New Genes FASTA' file *F >Found with R. suavis J3-A2 *F ATATAGTTCCATTCTGTTTTATTGGATTGAGTAAAGTTAGACAAAATGCAAGGTCTTGGTCTGCAAAGTCTTAAAAA *F AAA *F TCCAGCTTTAATTCCACTTTATGTGTGCGTTGGAGCGGGACTATTGGAGCCGTCTACTATATGGCTCGACTTGCTAC *F TCG *F TAATCCCGATGTCACTTGGAATCGCACATCAAATCCCGAACCATGGCAAGAGTACAAAGAAAAGCAATACAAGTTTT *F ATT *F CGCCTGTGAGGGATTATTCCAAAACTAAGAGTGCTGCCCCAAACTTTGATGAATAAATTACGTTTCCCTAGCAGCTG *F CAA *F TTTAAAAATGTAAAATGAAATAACTTCAAATTATAAATAAACATAGTGGATTTGAAAGCGTA *F >Extra1 *F ATGCTTAATCTCAACCTTCTAGATTGTATAGTTCCTGAGATCTCGACATTCATACAGACGGACGGACAGCGTCAGAT *F CGA *F CTCGGATATTGATCCTGATCGAGAATATATAGGCTTCATATGGTGA *F >Found with R. suavis J3-D1 *F ATGTATATTTATTTTCCAACAATCTTTCTCTTATTTTTGTATCCAGTAGTAGCAGTTGTCCCTCAAGGATTTACAAT *F TAA *F ACAACCAAAATGCTGGTATGTGGCAAACCCTGGACCCTGTGATGATTTTGTAAAAGTCTGGGGCTACGATTATTTGA *F CTA *F ATCGTTGCATTTTCTTTTATTATGGAGGCTGTGGTGGAAATCCAAATCGATTTTATACGAAAGAGGAGTGCTTGAAA *F ACA *F TGCCGTGTGTACAGACCTCCAAATCACGTCTGTTTGCTGCCAATCTGGGCGACGGCCATTAAGTCAAACCGTTTGAA *F GCA *F ATTTGAAAGCTACCCAGACTATGCAACATATATATTTTTACAGACTCCCTGGGTTATTTATCAACAATTTTATGTGG *F ATA *F GCGTTGCGATTCTGACAATTTTTGACATGCAATTTGCCATTTTCCATCTGCTTCAGCCGTATTTTGGGTGTGGAATT *F TGG *F CATTTTTCCGCAGGCTGCAATAAGTTTTGGCAGCGAATGCAGATGAGGCTCATGATGAGATGA *F >Found with R. suavis J3-A7 *F ATGATTGAAATATCAGATTTGCAGAAAATTGGCATCGGCTTGGCTGGTTTTGGCATTTTCTTTTTGTTTCTCGGCAT *F GCT *F GCTGCTGTTCGATAAAGGACTGCTCGCCATTGGCAATATTCTATTCATATCGGGCCTGGCCTGCGTCATTGGCGTGG *F AGC *F GCACGATGCGCTTTTTCTTCCAACGGCACAAAGTCAAAGGCACAACGGCCTTCTTAGGGGGAATCGTCATCGTCCTG *F CTG *F GGATTCCCCATCTTCGGCATGATTATTGAATCCTATGGATTTTTCGCACTCTTCAGCGGCTTCTTCCCCGTGGCCAT *F TAA *F TTTCCTAGGCCGAGTGCCTGTTTTAGGATCGCTGTTTAATTTACCATTTATACAAAAGATTGTTCAAAAACTTGGTG *F GAG *F ACGGCAACCGAACTACAGTAtaa *F >Found with R. suavis J3-B3 *F ATGGATGCACGAAAGTTTTCTACCCACATATTGGATACTTCGGTGGGAAAGGCGGCAGCCAATGTGAGAGTAACAGT *F TTC *F CAGGCTGGACGAGATTCAGGAATGGAGATCCCTTCGGGCGGCCCAAACTGATGCGGATGGTCGCTGCCTGCTCTTGG *F AAC *F CTGGTCAATTTCCCGGCGGGATCTATAAGCTGACCTTTCACGTGGGCGCCTATTACGCGGAGCGCAATGTGAGGACA *F CTT *F TATCCAGCAATTGACTTGATTGTGGATTGCAGTGAGAATCAGAACTATCACATTCCTTTGTTACTCAATCCCTTTGG *F GTA *F TTCCACATATCGTGGAACATAG *F >Found with A. mellifera Contig1312 *F ATGGACATCTCAAAGGCACCAAATCCGCGAAAACTGGAGCTGTGTCGCAAATACTTCTTTGCTGGCTTTGCATTTCT *F GCC *F CTTTGTGTGGGCCATTAACGTTTGCTGGTTTTTCACGGAGGCCTTCCATAAGCCACCATTTTCGGAGCAGAGCCAAA *F TAA *F AGAGATATGTTATATACTCTGCAGTGGGGACTCTATTCTGGCTGATAGTACTAACTGCCTGGATAATAATATTCCAG *F ACA *F AATCGCACAGCCTGGGGCGCCACAGCGGACTATATGAGCTTCATCATACCCCTAGGCAGTGCATAG *F >Found with A. mellifera Contig1481 *F TACCAATTACTTGTAAGCACAAAAAACAGCTGACGGCAACAAGTGGTTCGGTCCCCATCGGAATACACGTGCTCAAA *F ACG *F TGTGGGTTTTATTTGCCTTAATTGACTTAAATTCACTCGCAATAAGTGGAAATGATTCGAAAGGTGCCGCTAATTGT *F AGT *F CCTGGGCTCCACGGGCACCGGAAAGACGAAACTGTCTTTGCAACTGGCCGAACGCTTCGGAGGAGAAATAATCAGCG *F CTG *F ACTCCATGCAGGTTTACACCCACCTGGACATCGCCACCGCCAAGGCAACCAAGGAGGAGCAGTCCCGGGCACGACAT *F CAT *F CTACTGGACGTGGCCACACCGGCCGAACCCTTCACAGTCACTCACTTTCGTAACGCAGCACTGCCCATTGTGGAGCG *F CCT *F GCTCGCCAAGGACACTTCTCCGATTGTGGTGGGCGGCACGAATTACTACATAGAATCCCTACTTTGGGATATTCTGG *F TTG *F ACTCGGATGTCAAGCCGGACGAAGGCAAACATTCGGGGGAGCATCTTAAGGATGCCGAACTGAATGCTTTGTCCACC *F CTC *F GAGCTGCATCAGCACCTTGCCAAGATCGACGCAGGTAGTGCCAACCGTATTCACCCCAACAACCGGCGCAAGATCAT *F CCG *F GGCTATCGAAGTGTATCAGAGCACCGGGCAGACTTTGAGCCAGATGCTGGCGGAACAGCGGGCACAGCCGGGAGGAA *F ACC *F GCCTGGGTGGACCCCTTCGCTATCCACACATCGTTCTCCTTTGGTTGCGTTGCCAGCAGGATGTTCTAAACGAGCGA *F TTG *F GATTCCCGCGTAGATGGCATGCTGGCCCAAGGGCTGCTCCCTGAACTACGACAGTTTCACAATGCCCACCATGCTAC *F CAC *F TGTGCAAGCCTATACGTCGGGAGTTCTGCAGACGATTGGCTACAAGGAGTTTATTCCCTATCTGATCAAGTACGACC *F AGC *F AGCAGGACGAAAAGATAGAGGAGTACCTCAAAACCCATAGTTACAAGCTGCCAGGCCCAGAAAAACTGAAAGAAGAA *F GGT *F CTTCCAGATGGCTTGGAACTCCTACGCAATTGTTGCGAAGAACTAAAGTTAGTCACTCGCCGATACTCAAAGAAGCA *F GCT *F GAAGTGGATCAACAATCGATTCCTGGCCAGCAAAGATCGTCAAGTGCCGGATCTCTACGAACTGGACACCAGTGATG *F TGT *F CAGCTTGGCAGGTGGCAGTCTACAAGCGGGCAGAGACCATCATAGAAAGCTATCGAAACGAAGAGGCTTGCGAGATA *F CTA *F CCAATGGCCAAGCGGGAGCATCCTGGAGCGGATTTGGATGAGGAGACTAGCCATTTTTGTCAAATATGCGAACGGCA *F TTT *F CGTTGGGGAGTACCAATGGGGACTGCATATGAAGTCCAACAAACACAAGCGAAGAAAGGAGGGACAGCGCAAGCGGC *F AAA *F GGGATCACGAAACAATGCTCTCAACGGATCTAGCGAAGAAGCAAAAGGAGGAGAAAGAGGAGGCAGGAAAGGCGGAG *F ACT *F CAGCCACCACCCAGCCGAGTCAATGATACTGATAAGGCAATGtaa *F >Found with A. mellifera Contig2709 *F TTGAACACAGATGTCACTTCTACAGGGGAAAAAAGTTTAAAAACAAGTAAATCACAGAAAACGTCGTTTCCTTTTGC *F TAA *F TAGAGCGCCTGAATTCGGTGGAAATAGCAAAAATAATATATCACCATTCTTGGGACTGCAAACAAATTCGAAAATGA *F GTG *F ACAATTTTTCAAGAACACCATATTCTGATGGGCACGCTGCAACCCATGAGGAAGCATCAAAACCCCACTACACTACC *F ACT *F ACGAGTTCTTTTAGTAGAACTCCGGTCTCGCCGTACCTCAACTATGATTCGCGATATCTGCAGCAAGCACAGCCAGA *F GTT *F CATTTTTCCCGAAGGGGCCAACAAGCAGCGTGGACGCTTCGAGTTGGCCTTCTCTCAGATAGGCACTTCGGTAATGA *F TTG *F GCGGTGGAATTGGCGGCCTAGCAGGTGTTTATAATGGTTTAAAAGTCACAAAAGCACTCGAGCAGAAGGGAAAAGTT *F CGT *F CGAACACAGTTACTTAATCACATTATGAAGCAAGGTTCCGGCACAGCTAACACATTAGGTACATTGACGGTGCTGTA *F TTC *F GGCTTGTGGAGTTTTGCTGCAGTTTTTCCGCGGAGAAGATGATCATATAAACACAGTAATTGCGGGCTCTGCCACAG *F GAC *F TATTATACAAGTCAACAGCTGGCCTTAGGACGTGTGCTTTTGGTGGAGCTATTGGGCTGGGCATCTCGTCCCTCTAT *F TGC *F TTATACCTAATAGCACAGGAAAACAGTTCGAACTCAAGTCCCAAATACCTATAG *F >Found with A. mellifera BB260003B20H2.F *F ATGGTGGATTTTTTTGAAAAGCTTAGACGCGGTCACACATTTATTTACATCGAACATATGATGGGCACGCCGGAATT *F AAA *F AATCATATTAGAATTCAGTGCAGGGGCGGAGTTACTATTTGGTAACATAAAACGCCGTGAATTGAACTTGGACGGTA *F AAC *F AAAAATGGACTATTGCTAATCTGCTTAAGTGGATGCATGCGAATATTTTAACGGAGCGTCCGGAACTTTTTCTTCAA *F GGA *F GATACTGTGCGACCTGGAATTTTAGTACTCATAAATGATACAGACTGGGAATTGCTGGGTGAACTGGACTACGAGCT *F GCA *F GCCCAACGACAATGTGTTGTTTATATCAACTTTACACGGTGGTTAA *F >Found with A. mellifera BB260004B10A11.F *F ATGGAGAAATCTGAAATACGACTGCAACGCATGTCTAATGAATATCAGTCGCAATCGAGCTATATGTACCTCCGGAC *F CAA *F GATGCTGTTAAAAATCGAGAATACCCTACTTCGAAGCCATCGTCAGCGCGAGACCACCGGTATCAAGAAACTATACA *F ATT *F CGTTTTTCGTATTGTTTTAA *F >Found with A. mellifera BB260010A20C3.F *F TTCGCATATTTCAGTTATTTATTTAGAAATGGGGCGATTTAAGTTATGTGCTTCGCCGAGAGAGGTTATGAAGTACG *F AAG *F ACTTTATAAAACGCATTCGAAAAAGCCTCTACTATGGCGTTGGAACACCAGACACAGAAATGTCGGTCTCCTTACCC *F TTT *F GCGGAGTACGCGGCAGATTTGTTTTCGGAGACTCATCGCGGGCATTCTTTGCATCGCCTAAGTTGCGTATCTGCTGC *F ACA *F AGTACATGCCACGCCTTGCTCTTTAATTATGGCATTGATATACCTCGATCGCTTAAACGTCATCGACTCGGGCTATA *F GCT *F GCAGAATCACACCACAGCAGCTGTTTGTTGTGTCACTAATGATTTCCACAAAATTCTACGCGGGCCACGACGAACGG *F TTC *F TATCTGGAAGACTGGGCCAGTGACGCTTGTATGACGGAAGATAGGCTCAAGGCAGTCGAGCTCGAATTTCTTTCCGC *F TAT *F GGGTTGGAATATATACATATCCAATGAGCTATTCTTTGATAAGTTAAGAAACGTTGAACGTTCTTTGGCTGAACAGC *F AGG *F GACTGCGTAGAGGTTGGCTCACTTACAGTGAGCTCGTGCAGTTGCTGCCTAGCCTTGAATGGACGAAATTCCTCGTT *F AAC *F AGCCTGTCTGTACTATCTCTAAGCTATGCGGCAAGTATTATAACATTAGCCGGAGCTTTTTTTATTGCGAGCCAAGT *F TCC *F CGGTACGTTATGGCATCGGGATGTGGAAACTGCCTCAGATTTCACCATGACAATTAGCAGTCAGGTATCCGTTTCAA *F ATG *F CATTAGAGTCCACACCTTTTATTAATGTCCAAGTATCCTCACTTTTACGTAAAACGAGTAACGTGAATGTTGAATTG *F ATG *F AATCTTGAGAAGACAAGCTGCGCCAGGGCAAGACTGAATAAAATTGAATATAAGCATCCGCGCCATCAATCAGTACC *F TAC *F GCTTTCATTCATAAGCACCTGTCCACAACTTGATTTATTGTATGCCCAAGATGGAACAAGGAATTGGCTAAATATTA *F AAT *F CGCCCAACAGCGACTACAAAAACAACAGAAACCTTTCAATAACAGTTAGATCCGTACAACTAGAAGAGCAAAAGGCT *F GAA *F AATGATTCCGTTATTTGGCAAGCCAACACCGAAGCAATGCAGTAA *F >Found with A. mellifera BB260019B20F2.F *F ATGAAGGAAGAAGGCGGCACATTGCTGGGCGATAAAGGTGTACGAAGGCATCAGTCCATGCAGCGTCTGTCAGCGGA *F GCA *F GAATGGTGGTTCAACGACTGAACAAACACATGAACACAATCCAAACGTCGTACCTGATCATAGAGGCAACTTACACA *F TTA *F CAGTTAAGAAAACCAAACCAATTTTAGGTATTGCTATCGAAGGTGGTGCTAATACAAAACACCCGCTCCCTAGGATA *F ATC *F AATATCCATGAAAATGGTGCAGCATTTGAAGCGGGCGGCTTAGAAGTCGGCCAACTCATCCTGGAGGTAGATGGAAC *F GAA *F AGTGGAGGGTCTGCATCATCAGGAGGTTGCTCGACTAATAGCCGAATGCTTTGCTAATCGTGAAAAGGCTGAAATAA *F CCT *F TCTTAGTTGTCGAAGCAAAAAAATCAAATTTGGAACCGAAGCCGACGGCGCTGATATTTTTAGAAGCCTAA *F >Found with A. mellifera BB260023A20H5.F *F CTCTGTTTGAGGGCGTAGTTCCAACAAGTGCTGAGCATCACAATTTTCTATTACTAAGCCCAGCTTTGCGTTGGCGC *F GCC *F CCAGAATCTCATTTTATATTTAGTTTCTGCCAGTTTAGTTAATTAGTTAGTTGATAGTGTTGTTTGTTTCTTCTGCA *F ACA *F ATTGTGTGCGATAGGAGTCGGGCAAAATGTTCCCGTCGTCGATTTTGGGGCGCAGCTATTTGCTTTTTATGCTGGTG *F CTC *F GCCGTGGGCGTGTTCGCCCAACACGAGTGGCAGGCCCGGGATGCCTTTGATGAGATAAAGAGGCAGTTCGACAAGGT *F GAA *F CGCGGATAACTGCCCCATCCAACACCATTCGGACCTTTTCATGCCCATGGACGCGGTGTCCCACAAGCCGGACATCA *F AGG *F AGATCAACGTGAATCCGGTGTTCCCCAACCGAACTGCCCTGCTGCATCTGCAGAATATGGCCCTTAGCAGAAGCTTC *F TTC *F TGGAGCTACATCCTCCAGTCGAGGTTTATTCGACCCGCCATCAACGACACCTACGATCCCGGCATGATGTACTACTT *F TCT *F GTCCACCGTAGCCGATGTATCCGCCAACCCACATATCAACGCCTCGGCCGTGTACTTCTCCCCCAACAGCTCGTATT *F CGT *F CGTCGTATCGCGGCTTCTTCAATAAGACGTTCCCCAGATTCGGGCCAAGAACCTTCAGGCTGGACGACTTCAACGAT *F CCC *F ATTCATCTGCAGAAGATATCGACGTGGAATACTTTCGATGTTCAGGATCTGGGCGCCCATCACCCGGACTCCATATC *F CAA *F GGACTACACCCACGACCTGTATAAAATAAACGAGTGGTACCGCGCCTGGCTACCAGACAACGTCGAGGGACGGCACG *F ATA *F CGAAGATCACCTACCAGGTGGAAATCCGCTATGCGAACAACACAAACGAGACGTATACCTTCCACGGACCGCCTGGC *F TCT *F GAAGAAAACCCTGGTCCGATTAAATTTACAAGGCCGTACTTCGATTGTGGCAGGTCCAACAAGTGGCTGGTGGCCGC *F AGT *F AGTGCCAATTGCGGATATCTACCCCCGACACACGCAGTTCCGTCACATTGAGTATCCCAAATACACGGCCGTTTCGG *F TTC *F TTGAGATGGACTTCGAGCGTATCGACATAAACCAGTGTCCATTGGGTGAAGGCAACAAAGGACCTAATCACTTTGCG *F GAT *F ACGGCGCGGTGTAAAAAAGAAACGACAGAGTGTGAACCATTACAAGGCTGGGGCTTTAGGCGCGGTGGCTACCAGTG *F CCG *F TTGTAAGCCAGGTTTTCGGCTGCCCAACGTAGTGCGGCGACCTTATCTGGGCGAGATTGTGGAGCGCGCATCGGCAG *F AAC *F AGTACTACAACGAGTACGACTGCCTTAAGATTGGCTGGATCCAAAAGCTTCCCATTCAGTGGGATAAGGCCTCCTAC *F CAC *F ATTCGCCAAAAGTATCTGGACCGGCATCCGGAATATCGCAACTACACCACCGGCTCGCGATCACTTCATGCTGAGCA *F CTT *F AAATATTGATCAGGCGTTGAAGTATATTCATGGAGTCAACTATCGCACTTGCAAAAACTTCCATCCGCAGGATCTGA *F TTC *F TTCGCGGTGATGTGAGCTTCGGCGCCAAGGAGCAGTTCGAGAACGAAGCCAAGATGGCCGTGAGACTGGCCAACTTT *F ATT *F AGCGCCTTTCTGCAGAGTATGCAAACTATAACACGAATATCCTCCTTACAGGTATCGGATCCCAACGAAGTGTACTC *F GGG *F CAAGCGTGTGGCCGACAAGCCGCTGACCGAGGATCAAATGATCGGCGAGACCCTTGCCATTGTCCTGGGCGACAGCA *F AGG *F TTTGGTCGGCCACAATGCTCTGGGAGCGCAACAAGTTTACCAATCGCACATATTTCGCACCCTATGCCTACAAAACT *F GAG *F CTCAACACAAGAAAGTTCAAGGTGGAGGACCTGGCGCGGCTCAACAAGACGCACGAACTCTACACGGAAAAGAAGTA *F CTT *F CAAGTTCCTGAAGCAGCGCTGGAACACCAACTTCGACGACCTGGAGACCTTCTACATGAAGATCAAGATCCGCCACA *F ATG *F AAACAGGTGAATACCAGCAGAAGTACGAGCACTACCCAAATTCGTACAGAGCGGCCAACATCAAGCACGGCTACTGG *F ACT *F CAACCACAATTCGACTGCGATGGATATGTGAAGAAGTGGCTGGTGACCTATGCGGTGCCCTTCTTCGGCTGGGACAG *F CCT *F GAAAGTCAAGCTGGAATTCAAGGGTGTGGTAGCTGTCTCCATGGACATGCTGCAGCTGGACATCAACCAGTGCCCGG *F ACT *F GGTACTACGAACCGAACGCCTTTAAGAACACACACAAGTGTGACGAGCAATCGTCCTACTGCGTTCCCATTATGGGT *F CGT *F GGCTATGAAACCGGAGGCTACAAGTGCGAGTGCCTGCAGGGATACGAGTATCCTTTCGAGGATCTGATTACCTACTA *F CGA *F TGGACAGCTCGTCGAGGCCGAGTACCAAAATATTGTGGCTGATGTCGAGACCCGCTACGATATGTTCAAGTGCCGAC *F TGG *F CCGGAGCTTCGGGTCTGCAATCCGCTTTGGGACTTGTGGTCGCTCTGATCGGGCTCACGCTCACCCTGCTGTATAGA *F TTT *F AGTTAA *F >extra2 *F ATGGTCAAGCAAGTGGATTTTGCGGAGGTGAAGCTCAGTGAGAAATTTCTCGGAGCTGGATCTGGTGGAGCGGTGCG *F CAA *F AGCCACCTTTCAAAATCAGGAGATTGCAGTAAAGATATTTGATTTCCTTGAGGAAACAATCAAAAAGAATGCAGAGA *F GGG *F AAATCACACATTTGTCGGAGATCGACCACGAAAACGTTATCAGGGTGATCGGGAGGGCCAGCAATGGAAAGAAGGAC *F TAC *F TTGTTGATGGAGTACCTGGAGGAGGGGTCCCTCCACAACTACCTCTATGGCGATGACAAGTGGGAGTACACCGTGGA *F GCA *F AGCGGTTCGCTGGGCACTCCAATGCGCCAAGGCCTTAGCATACTTGCATTCGTTGGATCGACCGATTGTTCACCGCG *F ATA *F TTAAGCCGCAAAACATGCTTTTATATAATCAGCATGAAGACTTAAAGATTTGTGACTTTGGCCTGGCGACGGATATG *F TCC *F AATAATAAGACCGATATGCAAGGAACATTGAGGTATATGGCTCCCGAGGCCATTAAGCACTTAAAGTATACGGCTAA *F GTG *F TGATGTGTACAGCTTTGGAATAATGCTCTGGGAGCTGATGACACGTCAATTGCCATATAGTCACTTGGAAAACCCCA *F ACA *F GCCAGTACGCCATTATGAAAGCTATCAGTTCAGGCGAAAAACTTCCAATGGAAGCAGTAAGATCCGATTGCCCAGAG *F GGT *F ATCAAGCAATTAATGGAATGTTGCATGGATATAAATCCCGAAAAGCGCCCCTCTATGAAGGAGATCGAAAAGTTCCT *F TGG *F CGAACAGTATGAATCCGGCACTGACGAGGACTTTATCAAGCCTTTGGATGAGGATACCGTGGCTGTGGTGACCTACC *F ATG *F TGGATTCGTCCGGCAGCAGGATAATGCGTGTTGATTTCTGGCGACATCAGTTGCCATCGATCCGCATGACTTTTCCG *F ATA *F GTGAAACGGGAAGCCGAAAGATTGGGAAAGACCGTTGTCAGAGAAATGGCCAAGGCGGCGGCGGATGGAGATCGGGA *F AGT *F TCGGCGGGCTGAGAAGGACACGGAGCGTGAAACCTCGAGGGCTGCCCACAATGGAGAGCGGGAAACGCGGAGAGCGG *F GTC *F AGGATGTGGGTCGTGAAACTGTACGGGCGGTCAAGAAAATAGGAAAGAAACTGCGCTTCTAA *F >Found with A. mellifera BB270004B10G5.F *F CTGGAACAGACCGCATTCAGTGGCTCTTATCGGTAGAAACAGCAGCACTTTTCCGAGATGTCTATCAAATCCTTGAC *F ATA *F CGTTGCGATCTTTGGCCTTTTTTGGGGCTCAATTGCGGGAACTGTAGTTGATCAGTTTGGGATATATGGTGGTTCAC *F CGA *F TTACCACCACGGAAAGGAGTAATGCGGAGTTGCGCTGCATGAACATCAATCCGCAGAACTCGGTGGACTTGGAGCAG *F ATG *F ATGGGACTCTGGTACGGCAGCGAGATTATCGTGCACAGCCAAGATTTTCCGGGCACCTACGAGTACGACTCATGTGT *F CAT *F CATTCATCTGACCGATGCCACGGATCAGATCCGTTTGAGCCAAGCAAATCGCGGCTATGGCTATGGAAATCAGGACT *F ACA *F ACCGTAACCAGAATAACTATGGACGCACCACCACCACTCAATCCTCCTATCCGGATAGCGATGAGTACCCGTTGAGA *F TCG *F ATTCAAAGCCAGCAGAAGTACCTACGTTTGATTTGGAGTGAGCGTGATAACAATCTGGAGTATACTTTCAACTATAC *F CAC *F CAGTGCACCTGGTCAGTGGTCCAACATCGGCGATCAGCGGGGATCCTTGGTCACCCTGAACACGTACACCCAGTTCA *F CGG *F GCACTGTCCAGGTGGTGAAAGCGGTCAACGATCACCTGGTGCTGACCTTCTGCGGCAACGATGTTAAGAGCTCCATA *F TAC *F ACAGTGGTTCTCACCCGCAATCGCCTTGGTCTCAGTTTAGATGAGCTGCGTAGCATCAGGAATCTGCTTTCCCGCCG *F TGG *F ACTCTACACGGAGACCATTCGCAAGGTTTGCAATGGATGTGGGCGATTGGGTGGCAGCCTCTTCGCTCTTTTAGCCC *F TTT *F TGCTGGTCGTACGTTTGGCCTGGGGGCGTGGCCAGTGA *F >Found with A. mellifera BB270012B20H7.F *F GACACAATGAACTCACAAAAAGAATACGTATCGGACTGCGAAACCGACGATGATTATTATGTCGATTTGTTAACTTC *F AGG *F CAAGGGCAGTGATAAGAGTGAAAGTGATGTGTCGGACAAGTCTGAAAATTATCCAGGCCTAAAATCAAAGCATACTG *F CGA *F AGGCATTGCGGAAAACAAGGCATTGTGACGGCGATAATAGGGAATACAGGTCTAAGGAGTGCGACGACCTTCATTCC *F GAA *F GAGGAGTCTGAAAAATCGCGGTCGGATGCTTTATGGGCCGATTTTCTTGGCGACATTGATACTAAAAGCGTAATCAA *F CCA *F AAAAACAGATTATACGGAGGGAAACGCAGCAAGTGCTACCAATACCAATACGCATGAGACTTGTAATAAATATGATA *F AAA *F ACGATACGGCAATAATAAAAACTGCACAGCAATACGATTCCAAAAGAACCACGCTTTCAGTTTCCACACTCGGAAAA *F ATT *F AAACGATCATCCGCTGAAAAGAGTATCGGTACCATGATAAATAAATTTGAAAAGAAGAAAAAATTGACAGTGCTTGA *F AAG *F GTCACAATTGGATTGGAAAATATTTAAACAAGACGAAGGCATAGACGAACTTCTGTGCTCGCATAACAAAGGCAAGG *F ACG *F GGTATTTGGACCGTCAAGACTTTTTGGAGAGAACCGATCTTAGGCAGTTTGAAATGGAAAAGAAGTTGCGGCTGTCT *F CGC *F AGGCCATACTAA *F >Found with A. mellifera BB270013A20H11.F *F CTTCATTTAGGCTGGTTAGGTGGTTAATTCCATTTGTCTTCGTTCTTTTGTATTATTTTTACAAAGCGATAATATTT *F TAA *F TCGTTTATGATTATTACAATATAACAAAAAGTTAACATCTTTGGAATCTTAAAAATGAGTTTTCATTTTGCTGTACT *F GAC *F CCTTATTTTAACAGCCTTCACAGTTTCTCTGTGTGCTGAACAAAAAATTACAAAGAGTGACGCAGGTGAAATACGAA *F TTT *F TCAAACGTCTTATTCCTGCCGATGTTCTACGAGATTTTCCGGGAATGTGCTTTGCTTCAACTCGATGTGCCACTGTT *F GAG *F CCTGGAAAGTCGTGGGACCTTACTCCATTCTGCGGTCGATCTACTTGTGTTCAAAATGAGGAAAATGATGCAAAGCT *F ATT *F CGAACTCGTAGAAGACTGCGGCCCATTGCCACTGGCGAATGACAAATGTAAATTGGACACAGAGAAGACTAATAAAA *F CCG *F CATCGTTTCCTTATTGCTGCCCCATCTTTACATGTGACCCCGGTGTTAAATTGGAATACCCCGAGATCGGAAAGGAT *F AAT *F GACAAAAAGAATTCTGAGTGA *F >Found with A. mellifera BB270028A10H8.F *F ATGAAAAATGACAGCTGTTCGCTGCGAATGGCTATTTATGTTTGTTTTGACTCGGCTTTCGAATATATCGCAAAATA *F TAT *F ACAGGAAACATTTATATTCACAAAAATCTGTACGATGCACCCAGGGCAAATTGAGGTCAACGAGATCAATGGCTATT *F GGA *F CATTTCTGCTGAGCATCGATTGGAAGGATCCCTGGCTTATTGGCCTTATTTTGGCGCATATCTTAACCACCACCACT *F GCG *F CTGCTCAGCCGGAACAGCTCCAACTTCCAGGTTTTCCTCTTCCTAGTACTGTTGCTGGCAGTCTACTTCACCGAAAG *F CAT *F CAATGAGTTCGCTGCTAACAACTGGAGTTCCTTTTCCAGACAACAATACTTCGATAGCAACGGCCTGTTTATCTCGA *F CAG *F TTTTCTCAATACCTATTTTGCTTAATTGCATGCTTTTGATTGGCACTTGGCTCTACAACTCCACGCAGCTGATGGTG *F ACT *F CTAAAAACAGCGCAGCTCAAGGAGCGAGCTCGCAAGGAACGCCAGACTAAGGCGGATTCGGAATCCATAGCACATAA *F AAA *F GGCAGAGTAG *F >Found with R. suavis J3-A2 *F MQGLGLQSLKKNPALIPLYVCVGAGAIGAVYYMARLATRNPDVTWNRTSNPEPWQEYKEKQYKFYSPVRDYSKTKSA *F APN *F FDE *F >Extra1 *F MLNLNLLDCIVPEISTFIQTDGQRQIDSDIDPDREYIGFIW *F >Found with R. suavis J3-D1 *F MYIYFPTIFLLFLYPVVAVVPQGFTIKQPKCWYVANPGPCDDFVKVWGYDYLTNRCIFFYYGGCGGNPNRFYTKEEC *F LKT *F CRVYRPPNHVCLLPIWATAIKSNRLKQFESYPDYATYIFLQTPWVIYQQFYVDSVAILTIFDMQFAIFHLLQPYFGC *F GIW *F HFSAGCNKFWQRMQMRLMMR *F >Found with R. suavis J3-A7 *F MIEISDLQKIGIGLAGFGIFFLFLGMLLLFDKGLLAIGNILFISGLACVIGVERTMRFFFQRHKVKGTTAFLGGIVI *F VLL *F GFPIFGMIIESYGFFALFSGFFPVAINFLGRVPVLGSLFNLPFIQKIVQKLGGDGNRTTV *F >Found with R. suavis J3-B3 *F MDARKFSTHILDTSVGKAAANVRVTVSRLDEIQEWRSLRAAQTDADGRCLLLEPGQFPGGIYKLTFHVGAYYAERNV *F RTL *F YPAIDLIVDCSENQNYHIPLLLNPFGYSTYRGT *F >Found with A. mellifera Contig1312 *F MDISKAPNPRKLELCRKYFFAGFAFLPFVWAINVCWFFTEAFHKPPFSEQSQIKRYVIYSAVGTLFWLIVLTAWIII *F FQT *F NRTAWGATADYMSFIIPLGSA *F >Found with A. mellifera Contig1481 *F MIRKVPLIVVLGSTGTGKTKLSLQLAERFGGEIISADSMQVYTHLDIATAKATKEEQSRARHHLLDVATPAEPFTVT *F HFR *F NAALPIVERLLAKDTSPIVVGGTNYYIESLLWDILVDSDVKPDEGKHSGEHLKDAELNALSTLELHQHLAKIDAGSA *F NRI *F HPNNRRKIIRAIEVYQSTGQTLSQMLAEQRAQPGGNRLGGPLRYPHIVLLWLRCQQDVLNERLDSRVDGMLAQGLLP *F ELR *F QFHNAHHATTVQAYTSGVLQTIGYKEFIPYLIKYDQQQDEKIEEYLKTHSYKLPGPEKLKEEGLPDGLELLRNCCEE *F LKL *F VTRRYSKKQLKWINNRFLASKDRQVPDLYELDTSDVSAWQVAVYKRAETIIESYRNEEACEILPMAKREHPGADLDE *F ETS *F HFCQICERHFVGEYQWGLHMKSNKHKRRKEGQRKRQRDHETMLSTDLAKKQKEEKEEAGKAETQPPPSRVNDTDKAM *F >Found with A. mellifera Contig2709 *F MSDNFSRTPYSDGHAATHEEASKPHYTTTTSSFSRTPVSPYLNYDSRYLQQAQPEFIFPEGANKQRGRFELAFSQIG *F TSV *F MIGGGIGGLAGVYNGLKVTKALEQKGKVRRTQLLNHIMKQGSGTANTLGTLTVLYSACGVLLQFFRGEDDHINTVIA *F GSA *F TGLLYKSTAGLRTCAFGGAIGLGISSLYCLYLIAQENSSNSSPKYL *F >Found with A. mellifera BB260003B20H2.F *F MVDFFEKLRRGHTFIYIEHMMGTPELKIILEFSAGAELLFGNIKRRELNLDGKQKWTIANLLKWMHANILTERPELF *F LQG *F DTVRPGILVLINDTDWELLGELDYELQPNDNVLFISTLHGG *F >Found with A. mellifera BB260004B10A11.F *F MEKSEIRLQRMSNEYQSQSSYMYLRTKMLLKIENTLLRSHRQRETTGIKKLYNSFFVLF *F >Found with A. mellifera BB260010A20C3.F *F MGRFKLCASPREVMKYEDFIKRIRKSLYYGVGTPDTEMSVSLPFAEYAADLFSETHRGHSLHRLSCVSAAQVHATPC *F SLI *F MALIYLDRLNVIDSGYSCRITPQQLFVVSLMISTKFYAGHDERFYLEDWASDACMTEDRLKAVELEFLSAMGWNIYI *F SNE *F LFFDKLRNVERSLAEQQGLRRGWLTYSELVQLLPSLEWTKFLVNSLSVLSLSYAASIITLAGAFFIASQVPGTLWHR *F DVE *F TASDFTMTISSQVSVSNALESTPFINVQVSSLLRKTSNVNVELMNLEKTSCARARLNKIEYKHPRHQSVPTLSFIST *F CPQ *F LDLLYAQDGTRNWLNIKSPNSDYKNNRNLSITVRSVQLEEQKAENDSVIWQANTEAMQ *F >Found with A. mellifera BB260019B20F2.F *F MKEEGGTLLGDKGVRRHQSMQRLSAEQNGGSTTEQTHEHNPNVVPDHRGNLHITVKKTKPILGIAIEGGANTKHPLP *F RII *F NIHENGAAFEAGGLEVGQLILEVDGTKVEGLHHQEVARLIAECFANREKAEITFLVVEAKKSNLEPKPTALIFLEA *F >Found with A. mellifera BB260023A20H5.F *F MFPSSILGRSYLLFMLVLAVGVFAQHEWQARDAFDEIKRQFDKVNADNCPIQHHSDLFMPMDAVSHKPDIKEINVNP *F VFP *F NRTALLHLQNMALSRSFFWSYILQSRFIRPAINDTYDPGMMYYFLSTVADVSANPHINASAVYFSPNSSYSSSYRGF *F FNK *F TFPRFGPRTFRLDDFNDPIHLQKISTWNTFDVQDLGAHHPDSISKDYTHDLYKINEWYRAWLPDNVEGRHDTKITYQ *F VEI *F RYANNTNETYTFHGPPGSEENPGPIKFTRPYFDCGRSNKWLVAAVVPIADIYPRHTQFRHIEYPKYTAVSVLEMDFE *F RID *F INQCPLGEGNKGPNHFADTARCKKETTECEPLQGWGFRRGGYQCRCKPGFRLPNVVRRPYLGEIVERASAEQYYNEY *F DCL *F KIGWIQKLPIQWDKASYHIRQKYLDRHPEYRNYTTGSRSLHAEHLNIDQALKYIHGVNYRTCKNFHPQDLILRGDVS *F FGA *F KEQFENEAKMAVRLANFISAFLQSMQTITRISSLQVSDPNEVYSGKRVADKPLTEDQMIGETLAIVLGDSKVWSATM *F LWE *F RNKFTNRTYFAPYAYKTELNTRKFKVEDLARLNKTHELYTEKKYFKFLKQRWNTNFDDLETFYMKIKIRHNETGEYQ *F QKY *F EHYPNSYRAANIKHGYWTQPQFDCDGYVKKWLVTYAVPFFGWDSLKVKLEFKGVVAVSMDMLQLDINQCPDWYYEPN *F AFK *F NTHKCDEQSSYCVPIMGRGYETGGYKCECLQGYEYPFEDLITYYDGQLVEAEYQNIVADVETRYDMFKCRLAGASGL *F QSA *F LGLVVALIGLTLTLLYRFS *F >extra2 *F MVKQVDFAEVKLSEKFLGAGSGGAVRKATFQNQEIAVKIFDFLEETIKKNAEREITHLSEIDHENVIRVIGRASNGK *F KDY *F LLMEYLEEGSLHNYLYGDDKWEYTVEQAVRWALQCAKALAYLHSLDRPIVHRDIKPQNMLLYNQHEDLKICDFGLAT *F DMS *F NNKTDMQGTLRYMAPEAIKHLKYTAKCDVYSFGIMLWELMTRQLPYSHLENPNSQYAIMKAISSGEKLPMEAVRSDC *F PEG *F IKQLMECCMDINPEKRPSMKEIEKFLGEQYESGTDEDFIKPLDEDTVAVVTYHVDSSGSRIMRVDFWRHQLPSIRMT *F FPI *F VKREAERLGKTVVREMAKAAADGDREVRRAEKDTERETSRAAHNGERETRRAGQDVGRETVRAVKKIGKKLRF *F >Found with A. mellifera BB270004B10G5.F *F MSIKSLTYVAIFGLFWGSIAGTVVDQFGIYGGSPITTTERSNAELRCMNINPQNSVDLEQMMGLWYGSEIIVHSQDF *F PGT *F YEYDSCVIIHLTDATDQIRLSQANRGYGYGNQDYNRNQNNYGRTTTTQSSYPDSDEYPLRSIQSQQKYLRLIWSERD *F NNL *F EYTFNYTTSAPGQWSNIGDQRGSLVTLNTYTQFTGTVQVVKAVNDHLVLTFCGNDVKSSIYTVVLTRNRLGLSLDEL *F RSI *F RNLLSRRGLYTETIRKVCNGCGRLGGSLFALLALLLVVRLAWGRGQ *F >Found with A. mellifera BB270012B20H7.F *F MNSQKEYVSDCETDDDYYVDLLTSGKGSDKSESDVSDKSENYPGLKSKHTAKALRKTRHCDGDNREYRSKECDDLHS *F EEE *F SEKSRSDALWADFLGDIDTKSVINQKTDYTEGNAASATNTNTHETCNKYDKNDTAIIKTAQQYDSKRTTLSVSTLGK *F IKR *F SSAEKSIGTMINKFEKKKKLTVLERSQLDWKIFKQDEGIDELLCSHNKGKDGYLDRQDFLERTDLRQFEMEKKLRLS *F RRP *F Y *F >Found with A. mellifera BB270013A20H11.F *F MSFHFAVLTLILTAFTVSLCAEQKITKSDAGEIRIFKRLIPADVLRDFPGMCFASTRCATVEPGKSWDLTPFCGRST *F CVQ *F NEENDAKLFELVEDCGPLPLANDKCKLDTEKTNKTASFPYCCPIFTCDPGVKLEYPEIGKDNDKKNSE *F >Found with A. mellifera BB270028A10H8.F *F MKNDSCSLRMAIYVCFDSAFEYIAKYIQETFIFTKICTMHPGQIEVNEINGYWTFLLSIDWKDPWLIGLILAHILTT *F TTA *F LLSRNSSNFQVFLFLVLLLAVYFTESINEFAANNWSSFSRQQYFDSNGLFISTVFSIPILLNCMLLIGTWLYNSTQL *F MVT *F LKTAQLKERARKERQTKADSESIAHKKAE *F \----------------------------------------------------------------------------- *F \-- # *U FBrf0133243 *a Couso *b J.P. *t 1998.6.18 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Jun 18 17:16:33 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jun 1998 17:16:33 \+0100 *F To: j.couso@rhbnc.ac.uk *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 18 Jun 1998 17:26:34 \+0100 *F Content-Length: 1214 *F Dear Juan Pablo, *F I am curating your paper for FlyBase: *F Couso and Bishop, 1998, Int. J. Dev. Biol. 42(3): 345--352 *F and I have a question about the rn-lacZ construct in Figure 4C and D. *F FlyBase doesn't have a record of any rn-lacZ constructs, so I'd be grateful *F if you could tell me some details of it. *F 1. is it an enhancer trap, or a promoter fused directly to lacZ ? *F \-- if it is an enhancer trap, what enhancer trap construct is it, eg. lacW, *F PZ, and does it have a particular line designation, e.g. 24B, A101. *F \-- if the construct is a promoter fused directly to lacZ, how large is the *F promoter fragment, what vector is used (e.g. CaSpeR, Carnegie-20), what *F marker gene is used, and who made the construct ? *F 2. are there any references where it has been used ? *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From j.couso@rhbnc.ac.uk Thu Jul 30 12:37:28 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Jul 1998 12:37:28 \+0100 *F Date: Thu, 30 Jul 1998 23:49:36 \+0000 *F From: Juan Pablo Couso *F X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Dear Gillian, *F The rn-lacZ is an enhancer trap that we found during our screens. It is *F a P{lArB}ry+ enhancer trap inserted in the citological location of *F rotund, it has a weak rotund phenotype when homozigous (although it is *F viable and fertile) and it does not complement rotund alelles. We are *F trying to map it molecularly now. *F Juan Pablo. *F From gm119@gen.cam.ac.uk Thu Jul 30 15:18:23 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 30 Jul 1998 15:18:23 \+0100 *F To: j.couso@rhbnc.ac.uk *F Subject: Re: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 30 Jul 1998 15:17:43 \+0100 *F Content-Length: 219 *F Hi Juan Pablo, *F thanks for your e-mail. Do you have a particular number/letter designation *F for the rn-lacZ line so that I can record that it was mentioned in the *F Int. J. Dev. Biol. paper, *F Gillian *F From j.couso@rhbnc.ac.uk Mon Aug 24 11:49:23 1998 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 24 Aug 1998 11:49:23 \+0100 *F Date: Mon, 24 Aug 1998 23:01:56 \+0000 *F From: Juan Pablo Couso *F X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F We call that rn-lacZ line rn89. *F Best regards again, *F Juan Pablo. # *U FBrf0133244 *a Akam *b M. *t 1996.2.1 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Jan 09 15:02:42 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 9 Jan 2001 15:02:42 \+0000 *F Date: Tue, 9 Jan 2001 09:54:27 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: twi-GAL4-108.4 *F To: flybase-updates@morgan.harvard.edu *F X-Mailer: dtmail 1.3.0 CDE Version 1.3 SunOS 5.7 sun4m sparc *F Content-Type: text *F X-Sun-Text-Type: ascii *F Content-Length: 395 *F Personal communication from: Michael Akam, University of Cambridge *F To: Bloomington Drosophila Stock Center *F Subject: twi-GAL4-108.4 *F Dated: 1 February 1996 *F Background: The stock center has some information on this insertion that is *F not included in FlyBase. *F Information communicated: *F The insertion that FlyBase now calls P{GAL4-twi.G}108.4 is a homozygous *F viable insertion on chromosome 1. # *U FBrf0133245 *a Millburn *b G. *t 2001.1.15 *T personal communication to FlyBase *u FlyBase error report on Mon Jan 15 16:55:57 2001. *F From gm119@gen.cam.ac.uk Mon Jan 15 16:56:13 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 15 Jan 2001 16:56:13 \+0000 *F To: flybase-updates@morgan.harvard.edu *F Subject: scaffold: AE003241.2 *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 15 Jan 2001 16:55:57 \+0000 *F Content-Length: 1043 *F hello, *F I think that the scaffold AE003241.2, which is currently unnannotated *F and I think unlocalised may correspond to a sequence for an rRNA gene. *F If you do a BLASTN of the whole scaffold (its only 1136bp) against the *F nr database at the NCBI you get good hits to 3 Drosophila accessions: *F Score E *F Sequences producing significant alignments: (bits) Value *F gb|AE003241.2|AE003241 Drosophila melanogaster genomic scaf... 2252 0.0 *F gb|M21017.1|DRORGAB D.melanogaster 18S, 5.8S 2S and 28S rRN... 1181 0.0 *F emb|X70692.1|DM18SPSA D.melanogaster 18S rRNA pseudogene 979 0.0 *F emb|X15707.1|DMCBSRDNA D.melanogaster DNA sequence (isolate... 854 0.0 *F M21017 and X15707 are currently listed under bb (FBgn0020557) in FlyBase *F X70692 is listed under 'bb-psi' (bb pseudogene, FBgn0020557) *F hits to other species are also to 18S rRNA sequences, so I guess this *F scaffold could represent part of bb, bb-psi or Ybb (FBgn0016760) *F sequences. *F Gillian # *U FBrf0133265 *a Whitfield *b E. *t 2001.1.18 *T personal communication to FlyBase *u FlyBase error report for CG12348 on Thu Jan 18 01:23:32 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Jan 18 09:25:08 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jan 2001 09:25:08 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 18 Jan 2001 01:23:32 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG12348 on Thu Jan 18 01:23:32 2001 *F Content-Length: 710 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG12348 *F Gene annotation error *F Gene CG12348 has incorrect exon/intron structure. *F Comments: Celera have misannotated the Shaker gene at the N and C terminal exons *F (AE003507; AAF48786). There are also known to be 4 isoforms (alpha, beta, delta *F and epsilon). *F Please compare to: *F alpha \- *F Pongs et al, EMBO J. 7:1087-1096(1988), X07131; CAA30143 *F beta \- *F Pongs et al, EMBO J. 7:1087-1096(1988), X07132; CAA30144 *F delta \- *F Pongs et al, EMBO J. 7:1087-1096(1988), X07133; CAA30145 *F epsilon *F Schwarz et al, Nature 331:137-142(1988), X06742; CAA29917 *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133266 *a Whitfield *b E. *t 2001.1.18 *T personal communication to FlyBase *u FlyBase error report for CG18000 on Thu Jan 18 02:28:29 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Jan 18 10:29:49 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 18 Jan 2001 10:29:49 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 18 Jan 2001 02:28:29 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18000 on Thu Jan 18 02:28:29 2001 *F Content-Length: 912 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18000 *F Gene annotation error *F Gene CG18000 has incorrect exon/intron structure. *F Comments: Nurminsky et al, Mol. Cell. Biol. 18:6816-6825(1998) provide *F translation *F of the full length Cdic protein and evidence of 12 alternatively spliced *F isoforms (AF070687-AF070699). *F Celeras translation of Cdic (AE003572; AAF50928) is a little short and is *F missing the spliced isoforms. *F The N-terminal 1-233 aa are encoded on AE003572 but then the DNA runs out *F so fails to encode the remainder of the protein. The C terminal 387-661aa *F are on AE002611. I cannot find the internal fragment for 234-386aa, could *F this region be targetted for resequence please. *F I guess the ends of these accession should be revised so a join for Cdic is *F not required. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133267 *a Whitfield *b E. *t 2001.1.30 *T personal communication to FlyBase *u FlyBase error report for CG4215 on Tue Jan 30 07:59:46 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jan 30 15:59:51 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 Jan 2001 15:59:51 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 30 Jan 2001 07:59:46 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4215 on Tue Jan 30 07:59:46 2001 *F Content-Length: 397 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4215 *F Gene annotation error *F Gene CG4215 has incorrect exon/intron structure. *F Comments: Celera defines an extra in frame exon which means the translation *F loses *F 63 amino acids. Otherwise the translation is tickety boo! *F thanks *F Ele *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0133268 *a Whitfield *b E. *t 2001.1.4 *T personal communication to FlyBase *u FlyBase error report on Thu Jan 4 16:31:21 2001. *F From eleanor@ebi.ac.uk Thu Jan 04 16:34:19 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 4 Jan 2001 16:34:19 \+0000 *F Date: Thu, 04 Jan 2001 16:31:21 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 en (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F Subject: Celera sequence for Hsp70 genes *F Content-Transfer-Encoding: 7bit *F I have been cleaning up the heat shock entries in Swiss-prot and TrEMBL *F and I found that Celera had only annotated one of the Hsp70 genes. *F CG18743 corresponds to Hsp70Aa, it is a better amino acid match to *F Hsp70Aa than Hsp70Ab, but if you believe differently please let me *F know. *F By translating the entire DNA sequence from AE003693 and AE003696 in all *F 6 frames I found bits and pieces of all 5 genes but both gene clusters *F were in regions of really low sequence quality, lots of Xs. I could *F translate a complete Hsp70B gene (all three have the same sequence so I *F am unsure which it corresponded to) but it involved 3 frameshifts when *F the translation should be contained wholly within 1 coding exon. *F I am not altogether sure of your strategy regarding any further *F sequencing but from reading *F http://www.fruitfly.org/sequence/assembly.html it seems the whole genome *F will be re-examined, not targetted sites. *F Anyway, the point of this message it to request that these two GenBank *F entries will have their sequence examined as the Hsp70s really should be *F annotated. *F Nellie # *U FBrf0133269 *a Whitfield *b E. *c G. *d Millburn *t 2001.2.1 *T personal communication to FlyBase *u FlyBase error report for Parp on Thu Feb 1 05:51:55 2001. *F From eleanor@ebi.ac.uk Tue Dec 19 10:01:34 2000 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 19 Dec 2000 10:01:34 \+0000 *F Date: Tue, 19 Dec 2000 10:01:06 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 en (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: Parp \- finding all the exons! *F Content-Transfer-Encoding: 7bit *F Hi, *F this is a good one \- I enjoyed figuring it out. *F Parp, currently is said to be at 81F and has synonym CG17718. *F Parp is 994 aa long and CG17718 (AE002892; AAF45445) corresponds to the *F C-terminal 375-994. Please note that the CDS feature only defines 557aa *F but the translation can be extended to cover 375-994. *F By sequence similarity I found the N-terminal 197aa in CG17696 *F (AE002935; AAF45400). *F Neither AE002892 or AE002935 has the internal fragment of aa198-374. So *F by BLASTn I found this last stretch on AE002666. *F so: *F AE002935 1-197 *F AE002666 198-374 *F AE002892 375-994 *F >From your reannotation proposal this is going to link up at least 3 *F contigs into one entry (so as not to have join statements and the *F maximum length of 350kb can be exceeded if one gene exceeds the *F length). I do not know of any intervening accession as I do not have an *F assembled map. *F Also, Parp is said to be at 81F by *F FBrf0102327 == Hanai et al., 1998, J. Biol. Chem. 273(19): 11881--11886 *F in situ hybridisation, data not shown. *F I wonder if they got the right chromosome arm as AE002935, AE002666 and *F AE002892 all map to 41C-D. (AE002892 does have two locations, 81F is *F also listed as it has annotation for CG17718). *F Ele *F From FlyBase-error@hedgehog.lbl.gov Thu Feb 01 13:51:58 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 1 Feb 2001 13:51:58 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 1 Feb 2001 05:51:55 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG17685 on Thu Feb 1 05:51:55 2001 *F Content-Length: 566 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG17685 *F Gene annotation error *F Gene CG17685 corresponds to D13806 (Parp (FBgn0010247)) *F Comments: Hi, *F further to what Eleanor found out about the Parp gene (see e-mail 19 *F Dec 2000 'Subject: Parp \- finding all the exons!') CG17685 *F (FBgn0039975) corresponds to the middle section of the Parp gene on *F scaffold AE002666. (Note, this CG is only present in release 1 NOT *F release 2), *F Gillian # *U FBrf0133270 *a Hanai *b S. *t 2001.1.11 *T personal communication to FlyBase *u FlyBase error report for AE002935 on Wed Jan 10 18:14:20 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Jan 11 02:14:28 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 11 Jan 2001 02:14:28 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 Jan 2001 18:14:20 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: shanai@md.tsukuba.ac.jp *F Subject: FlyBase error report for AE002935 on Wed Jan 10 18:14:20 2001 *F Content-Length: 1329 *F Error report from Shuji HANAI (shanai@md.tsukuba.ac.jp) *F Gene or accession: AE002935 *F Assembly error *F Comments: AE002935 is a part of PARP gene at 81F on 3R NOT 2R. PARP is *F terribly large gene more than 60 kb, and is singl copy gene: PMID: 9565614. *F CG17696 gene is exon 1, 2, 3 of PARP. I confirmed the map position on PARP by *F PCR. Embryo from Df(3R)10-65/blueBal clossed with Df(3R)4-75/blueBal was *F stained with X-gal. Df/Df developed normally until final stage of *F embryogenesis. PCR using PARP primers for each exons showed stained embryo, *F blueBal/blueBal and blueBal/Df ,have all exons and unstained embryo, Df/Df, *F lacks all exons of PARP. DNA extraction was confirmed by PCR using ftz primers *F as internal control. *F AE002666 have exon 4 of PARP at position 9658...10191 in sense direction. So *F that it must be on 3R NOT 2R. Distance between exon 3 and 4 is ~36 kb, so that *F there is a ~16 kb gap between AE002935(complement) and AE002666. *F AE002892.2 is have PARP gene as notes by BDGP, however predicted start *F position is not correct. It is mysterious that both map='41C-41D' *F map='81F-81F' are in 'source' of the note. Distance between exon 4 and 5 is *F >20 kb, so that there is a >5 kb gap between AE002666 and AE002892.2. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: FBupdate, /www/hedgehog_8001/htdocs # *U FBrf0133271 *a Goldin *b E. *t 2001.1.23 *T personal communication to FlyBase *u FlyBase error report for CG8743 on Tue Jan 23 13:33:16 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jan 23 21:33:21 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 23 Jan 2001 21:33:21 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 23 Jan 2001 13:33:16 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: goldin@codon.nih.gov *F Subject: FlyBase error report for CG8743 on Tue Jan 23 13:33:16 2001 *F Content-Length: 340 *F Error report from Ehud Goldin (goldin@codon.nih.gov) *F Gene or accession: CG8743 *F Missed gene *F Comments: CG8743 is probably homologous to human mucolipin NM_020533 (Sun et *F al. Hum. Mol. Gen. 2000, Vol. 9, No. 17 2471-2478 # *U FBrf0133272 *a Mazzarelli *b J. *t 2001.1.25 *T personal communication to FlyBase *u FlyBase error report for CG4252 on Thu Jan 25 09:51:58 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Jan 25 17:52:04 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 Jan 2001 17:52:04 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 25 Jan 2001 09:51:58 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mazz@snowball.pcbi.upenn.edu *F Subject: FlyBase error report for CG4252 on Thu Jan 25 09:51:58 2001 *F Content-Length: 281 *F Error report from Joan Mazzarelli (mazz@pcbi.upenn.edu) *F Gene or accession: CG4252 *F Missed gene *F Comments: sequence has similarity to ProDom protein domain (PD000781)which is *F a kinase family *F Browser: Mozilla/4.7 en (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0133273 *a Whitfield *b E. *t 2001.1.12 *T personal communication to FlyBase *u *F From eleanor@ebi.ac.uk Fri Jan 12 16:22:39 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 12 Jan 2001 16:22:39 \+0000 *F Date: Fri, 12 Jan 2001 16:23:50 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 en (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: LysA and LysC in Celera sequence *F Content-Type: multipart/alternative; *F boundary='------------46033709FE3ADEC0E95E1898' *F Content-Length: 3045 *F Hi peeps, *F Just to let you know my latest discovery! *F Currently LysA has no CG and I may have found the translation in *F AE003470. *F Between nucleotides 280215 and 279800 in frame 6 you can translate a *F 140aa protein: *F MKAFIVLVAL ALAAPALGRT MDRCSLAREM SNLGVPRDQL ARWACIAEHE SSYRTGVVGP *F ENYNGSNDYG IFQINDYYWC APPSGRFSYN ECGLSCNALL TDDITHSVRC AQKVLSQQGW *F SAWSTWHYCS GWLPSIDDCF *F Looking at the EMBL entry there is no CDS predicted at these positions. *F My only concern is that I do not know the order of the Lysozymes on the *F chromosome and therefore if this translation is in the correct place and *F orientation to be LysA. *F The translation does perfectly match the only other sequence for LysA *F from Daffre et al, Mol. Gen. Genet. 242:152-162(1994), Z22223; CAA80225. *F The one complication is that the translations for LysA and LysC are *F identical. LysC translation in *F Celera is incorrect and I cannot find the correct sequence (any idea why *F not? It is not obvious to me unless this is another case of low copy *F tandem repeats causing assembly problems?) so I currently I am unable to *F say for sure which translation belongs to which gene. *F I hope this info helps someone! *F thanks *F Nellie # *U FBrf0133274 *a Levis *b R. *t 2001.1.24 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Wed Jan 24 19:30:10 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 24 Jan 2001 19:30:10 \+0000 *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Wed, 24 Jan 2001 14:31:00 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: P-insertion in Pdk missing from GeneSeen *F Cc: Allan Spradling , *F *F The BDGP database contains a 100 nt flanking sequence for P-element *F insertion EP(2)0547, which gives a BLAST hit to the gene CG8808 (= *F Pdk). *F >CG8808|FBgn0017558|CT3054|FBan0008808 last_updated:000321 *F Length = 2560 *F Minus Strand HSPs: *F Score = 348 (52.2 bits), Expect = 2.4e-13, Sum P(2) = 2.4e-13 *F Identities = 74/78 (94%), Positives = 74/78 (94%), Strand = Minus / Plus *F Query: 77 TCGG-CGTTGGAGAATCCAGAGCCACCTGGAGTCGCATTCTCAATCCGAATTCCCAACCG 19 *F TCG C TTGGAGAATCCAGAGCCACCTGGAGTCGCATTCT AATCCGAATTCCCAACCG *F Sbjct: 46 TCGAACATTGGAGAATCCAGAGCCACCTGGAGTCGCATTCTTAATCCGAATTCCCAACCG 105 *F Query: 18 TTCGCGTCAAAGTCGCGC 1 *F TTCGCGTCAAAGTCGCGC *F Sbjct: 106 TTCGCGTCAAAGTCGCGC 123 *F Score = 155 (23.3 bits), Expect = 2.4e-13, Sum P(2) = 2.4e-13 *F Identities = 31/31 (100%), Positives = 31/31 (100%), Strand = Minus / Plus *F Query: 100 GCGCTCGCAGCTTTTAGGTAAAATCGGCGTT 70 *F ||||||||||||||||||||||||||||||| *F Sbjct: 12 GCGCTCGCAGCTTTTAGGTAAAATCGGCGTT 42 *F However, this P insertion is not displayed on the GeneSeen map of *F this region, which shows no P element insertions in the Pdk gene. My *F suspicion is that it has not been mapped on GeneSeen because the *F alignment of the flanking sequence with the genomic sequence has a *F discontinuity. Further support for this insertion site is that *F EP(2)0547 has been mapped by in situ hybridization to 45D8-9, which *F is consistent with the cytogenetic location of Pdk. *F Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210 *F phone: (410) 554-1238 *F fax: (410) 467-1147 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0133275 *a Da Lage *b J.L. *t 2001.1.24 *T personal communication to FlyBase *u *F >From Jean-Luc.Da_lage@pge.cnrs-gif.fr Wed Jan 24 12:26:17 2001 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 24 Jan 2001 12:26:17 \+0000 *F X-Sender: jldl@mailhost.pge.cnrs-gif.fr *F Mime-Version: 1.0 *F X-Mailer: Eudora Light F3.1l *F Date: Wed, 24 Jan 2001 13:29:27 \+0100 *F To: Michael Ashburner (Genetics) *F From: Jean-Luc Da Lage *F Subject: Re: Help FlyBase please *F >Dear Jean-Luc *F > *F >I wish to curate your new EMBL records AF238900 \- AF238977 for FlyBase. *F >But the records do not say which of the seven ananassae amylase genes *F >each corresponds to. Could you help me please. *F Dear Pr. Ashburner, *F .. *F The accession numbers AF238900 to *F AF238957 refer to Amy35 ; AF238958 to AF238977 refer to Amy58 (upstream *F regions and beginning of the genes in various strains) *F > *F > *F >Also could you tell me what the real names of these genes are: *F > *F >Dana\Amy1 37C *F >Dana\Amy2 37C *F >Dana\Amy4 37C *F >Dana\Amy3 81D *F I don't understand that well. Amy1 (or rather AMY1) is the protein encoded *F by Amy35; AMY2 by Amy58; both at position 37C; AMY3 is encoded by Amyi5; *F AMY4 by Amy4N, both at position 81D (see Da Lage et al, J. Mol Evol issue *F of october 2000). *F >Dana\AmyTBU118 AB003755; BAA22481 *F > *F AmyTBU118 is from Nobuyuki Inomata (Kyushu Univ, Fukuoka). It looks like *F Amy35 or Amy58 or Amy2E, but is slightly different from all of them. It is *F a partial sequence. *F > *F >These are the other genes in this family of ananassae amylases that *F >FB knows about. *F > *F >Dana\Amy2E 37C U53480; AAB69331 *F >Dana\Amy35 37C U31122; AAC47353;; *F >U53698; AAC79123 *F >Dana\Amy58 37C U53698; AAC79122 *F > *F >Dana\Amy4N 81D U31121; AAC47352;; *F >U53477; AAC48343 *F >Dana\Amyi5 81D U53478; AAB69330 *F > *F >Dana\Amyc1 74A U31123; AAC47354;; *F >U53699; AAC35243 *F >Dana\Amyc6=Amyrel 76C AF024691; AAC39090 *F > *F All these data are from my work and seem correct. *F > *F >Your help would be much appreciated. *F > *F >Thankyou *F > *F >Michael Ashburner *F Jean-Luc Da Lage *F Jean-Luc Da Lage *F UPR 9034 Populations, Genetique et Evolution *F Centre National de la Recherche Scientifique *F 91198 Gif sur Yvette Cedex *F France # *U FBrf0133281 *a Dickson *b B.J. *c K.A. *d Senti *t 2001.2.6 *T personal communication to FlyBase *u *F From dickson@nt.imp.univie.ac.at Tue Feb 06 17:02:22 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 6 Feb 2001 17:02:22 \+0000 *F Mime-Version: 1.0 *F Date: Tue, 6 Feb 2001 18:07:17 \+0100 *F To: Gillian Millburn (Genetics) *F From: Barry Dickson *F Subject: Re: FlyBase query *F Dear Gillian, *F a,b: Yes. Sema2b enhancer, hsp70 promoter, tau-myc coding seq. *F c: CaSpeR derivative (white+) *F Thanks, *F Barry *F >Dear Dr. Dickson, *F > *F >I am curating your paper for FlyBase: *F > *F >Rajagopalan et al., 2000, Cell 103(7): 1033-1045 *F > *F >and I have a question about the 'Sema2b-tau myc' cell marker you used *F >(page 1037). *F > *F >I would be grateful if you could tell me some details of this *F >construct, so that I can record it in FlyBase: *F > *F >a. what are the promoter sequences driving expression of this marker \- *F >are they Sema2b sequences ? *F > *F >b. what coding sequences are present \- presumably tau sequences and *F >also sequences encoding the myc tag ? *F > *F >c. what vector is the construct in e.g. CaSpeR, Carnegie-20 (so I can *F >work out what the marker allele is.) *F > *F >Any information you give me would be recorded as a personal *F >communication to FlyBase, and since this marker is cited as 'K.A. Senti *F >B.J. Dickson unpublished results', perhaps the best thing would be to *F >have both you and K.A. Senti as authors of this personal communication? *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F \-------------------------------------------------- *F Barry Dickson *F Research Institute of Molecular Pathology (I.M.P.) *F Dr. Bohr-Gasse 7 *F A-1030 Vienna *F Austria *F Tel. \+43 1 797 30 421 *F Fax. \+43 1 798 71 53 *F Email. dickson@nt.imp.univie.ac.at *F \-------------------------------------------------- # *U FBrf0133282 *a Lemaitre *b B. *t 2000.12.20 *T personal communication to FlyBase *u *F Archived. # *U FBrf0133285 *a Bellen *b H. *t 2000.12.25 *T personal communication to FlyBase *u *F From hbellen@bcm.tmc.edu Mon Dec 25 23:05:33 2000 *F To: rd120@gen.cam.ac.uk *F Subject: numerous errors in annotation of genes *F Cc: ma11@gen.cam.ac.uk *F Rachel, *F . *F . *F Re the Verstreken and Bellen p.c. *F . *F csp is not a proteolytic enzyme but a chaperone *F syt is not a transporter and is not a protein kinase *F syn is not involved in DNA repair *F bap is not a transporter *F sed5 is involved in vesicle fusion not targeting *F . *F n-syb is not a SNAp receptor , nor a transporter *F CAKI is not involved in the cell cycle to my knowledge *F CDK5 is thought to be involved in neurotransmitter release *F NSF2 is not a transporter *F rab2 and rab5 and rab10 and rab11 and rab14 are not involved in cell *F growth and ...intracellular what?... *F alpha adaptin is not a transporter *F AP2 is an adaptor protein , not a transcription factor. That is another AP2. *F AP47 is not a transporter *F . *F unc 13 is the name of the c. elegans gene and should be changed to *F Dunc13 to avoid confusion. It is not a protein Kinase and it is not *F required for cell growth *F amphiphysin is not involved in signalling in signal transduction. It *F is component that binds dynamin and plays probably a role in *F endocytosis *F We will stop here, but numerous others are wrongly annotated. We *F will continue when these are fixed. *F Hugo and Patrik *F Hugo J. Bellen *F Investigator, Howard Hughes Medical Institute *F Charles Darwin Chair in Genetics *F March of Dimes Chair in Developmental Biology *F Director, Program in Developmental Biology *F Baylor College of Medicine *F One Baylor Plaza, T630 *F Houston, TX 77030 *F Tel. 713-798-5272 *F Fax. 713-798-3694 *F email. hbellen@bcm.tmc.edu # *U FBrf0133286 *a Bodai *b L. *t 2000.12.22 *T personal communication to FlyBase *u *F From lbodai@uci.edu Fri Dec 22 20:08:54 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Sin3A-EcR interaction *F Dear Sir/Madam, *F I found a following genetic interaction in your Sin3A (and EcR) report: *F Tsai et al., 1999*: Only 14% of EcRE261st/Sin3Ak07401 flies derived from a *F cross of Sin3Ak07401 females to EcRE261st males survive (significantly *F lower than the expected 33.3%). Escapers show delayed development and wing *F defects; the wings are held horizontally at a 45-90o angle from the body *F axis. None of the EcRE261st/Sin3Ak07401 progeny of a cross of EcRE261st *F females to Sin3Ak07401 males survive to adulthood. *F I decided to check this interaction with different alleles therefore *F crossed EcRC300Y/BcGla females with Sin3A08269/Cyo,ry males. After scoring *F 288 F1 animals I couldn't see any reducement in the viability of *F EcRC300Y/Sin3A08269 progenies (102 flies with this phenotype emerged) and *F they don't have any visible phenotype. *F So it seems to me that the earlier reported interaction was caused by some *F background lethality. *F Best regards *F Laszlo Bodai *F Laszlo Bodai *F Dept. Developmental and Cell Biology *F University of California Irvine *F lbodai@uci.edu # *U FBrf0133287 *a Sung *b C. *c S. *d Robinow *t 2000.12.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Dec 22 20:54:55 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{EcR.GET-BD-GAL4}1 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Carl Sung and Steve Rabinow, University of Hawaii *F (12/00). P{EcR.GET-BD-GAL4}1 is a homozygous viable and fertile, second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0133288 *a Carpenter *b A. *c Y.H. *d Sun *t 2000.12.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Dec 22 21:11:19 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(3L)F10 and Df(3L)E44 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Adelaide Carpenter, University of Cambridge (12/00). *F Df(3L)F10 was isolated by Y. Henry Sun, Academia Sinica, from irradiation *F of the P{lacW}Eq-1 chromosome. Adelaide Carpenter examined polytene *F chromosomes and determined that it has breakpoints 69A2;69D1; the 69A1 and *F 69D2-3 bands are still present. *F Df(3L)E44 was also isolated by Y. Henry Sun. Adelaide Carpenter determined *F that it has breakpoints 69D2;69E3-5. *F Df(3L)F10 fails to complement Df(3L)E44. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0133289 *a Wharton *b R. *t 2000.12.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Dec 22 21:29:42 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: 66EF genes and alleles from Robin Wharton *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Robin Wharton, Duke University (12/00). *F A wild type third chromosome was mutagenized with EMS and lethal and female *F sterile mutations were isolated that failed to complement *F Df(3L)Scf-R6. Approximately 5000 chromosomes were screened. This screen *F was briefly described in Dahanukar et al. 1999 FBrf0111352 as the source *F of the smg1 mutation. The lethals and female steriles were placed into *F 13 complementation groups including smg. Alleles include *F l(3)66EFaLJF1 *F l(3)66EFbL1 *F l(3)66EFcL3 *F l(3)66EFdL4 *F l(3)66EFeL7 *F l(3)66EFfL9 *F l(3)66EFgL10 *F l(3)66EFhL16 *F l(3)66EFiL17 *F l(3)66EFjL27 *F fs(3)66EFaS17 *F fs(3)66EFbS18 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0133290 *a Bejsovec *b A. *t 2000.12.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 28 16:47:46 2000 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-wg.H.T:HA1}6C and P{UAS-wg.H.T:HA1}3C insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Amy Bejsovec, Duke University (12/00). *F P{UAS-wg.H.T:HA1}6C is a homozygous viable and fertile third chromosome *F insertion. *F P{UAS-wg.H.T:HA1}3C is a homozygous viable and fertile second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0133291 *a Luo *b L. *t 2000.1.9 *T personal communication to FlyBase *u *F From lluo@stanford.edu Tue Jan 09 05:02:13 2001 *F To: Michael Ashburner (Genetics) *F Subject: Re: genghis khan *F >Liqun *F > *F >I think that we are OK. *F > *F >We have now in FB: *F > *F >gek = CG4012, encoding a protein serine/threonine kinase *F > *F >and *F > *F >CG11290, encoding a probable histone acetyltransferase. *F > *F >If you can confirm in an email to me that CG11290 = enoki mushroom (enok) *F >and that this is probably a histone acetyltransferase then we *F >will curate the rest when you paper comes out. *F > *F >Many thanks *F > *F >Michael *F Dear Michael, *F CG11290 is indeed enoki mushroom. *F Thanks for your attention. *F Liqun *F =================================================================== *F Liqun Luo, Ph. D. Tel: (650)723-6645 *F Assistant Professor Fax: (650)723-0589 *F Department of Biological Sciences Dept Fax: (650)723-6132 *F Herrin Labs 144A E-mail: lluo@stanford.edu *F 385 Serra Mall *F Stanford University *F Stanford, CA 94305-5020 *F WWW: http://www.stanford.edu/group/luolab *F =================================================================== # *U FBrf0133292 *a Ghazi *b A. *t 2001.1.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Jan 22 11:29:17 2001 *F To: vijay@ncbs.res.in *F Subject: Helping FlyBase: srGAL4 *F Dear Vijay, *F I am curating this paper of yours, for FlyBase: *F \*x FBrf0131298 == Ghazi et al., 2000, Development 127(24): 5309--5318 *F I have a couple of questions, about the srGAL4 and the UAS-GFP you *F use. *F We have no record of an srGAL4 in FlyBase so I wondering whether you *F would be able to tell me whether this is an enhancer trap type *F insertion of P{GawB}? (If it is from the big Madrid screen then do you *F know the identifier number for it? We may already have a record of *F it, but not yet been told that the insertion falls in sr). *F Alternatively it could be a promoter fusion construct, in which case *F FlyBase would store the data in a different format \- which is why I ask *F for the clarification. *F Also, we have several UAS-GFP constructs. Which UAS-GFP is the one *F recombined onto the srGAL4 chromosome? If you could give me either a *F Bloomington stock number, or a reference to a paper where it has *F previously been used then I would be able to figure it out. *F Many thanks for your help, *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From aghazi@mailsvr.ncbs.res.in Mon Jan 22 12:41:28 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: srGAL4 *F Dear Dr. Drysdale, *F Thank you very much for your mail and for curating our publication for *F flybase. In answer to your queries, *F \-Yes, srGAL4 is from the big morata/madrid screen. Identification number *F is md710. *F \-However, the construct we've used in our paper is a RECOMBINANT OF *F 'SRGAL4 MD710' WITH UAS-GFP ON THE THIRD CHROMOSOME and NOT md710 in its *F original form. *F \-This recombinant was generated by Daniela Pistillo is the laboratory of *F Pat Simpson now at Cambridge and was given to us before *F publication. This is the reason you could not find it described anywhere. *F \-I'm afraid I DO NOT know the nature of the UAS-GFP used for the *F recombination but can ask Daniela if you think the information is crucial. *F I do hope I've been able to answer your questions satisfactorily. Please *F do let me know if I can be of any more help. Thanks once again for your *F efforts with our publication. *F Best regards, *F Arjumand # *U FBrf0133293 *a Giesen *b K. *t 2001.1.24 *T personal communication to FlyBase *u *F From KayGiesen@web.de Wed Jan 24 19:53:13 2001 *F To: *F The gene shroud (sro) is located in 99E5-F1 by inclusion of the deficiencies *F Df(3R)L127, A113 and R133. *F _________________________________________________ *F The gene spook (spo) is located in 64E by inclusion of the deficiencies *F Df(3L)vn-gamma3 and v65c and exclusion of vn-ry (64F). *F The deficiency Df(3L)vn-ry was provided to us by Linda Hall together with *F the deficiencies vn-gamma1, gamma2 and gamma3. I don't know if they're *F published but the cytology was confirmed by chromosome analysis. *F _________________________________________________ *F The gene shadow (sad) is located in 86F6-87A2 by exclusion of Df(3R)kar-H5. *F _________________________________________________ *F The gene haunted (hau) is located in 85D by P-Element-location and in *F 85C1-2;D8 by inclusion of the deficiency Df(3R)by10 and exclusion of *F Df(3R)pXT103. *F _________________________________________________ *F The gene shade(shd) is located in 70D5-E5 by inclusion of the deficiency *F Df(3L)fz-GS1a and exclusion of Df(3L)fz-GF3b. *F _________________________________________________ *F Dr. Kay Giesen # *U FBrf0133294 *a Struhl *b G. *c G. *d Campbell *t 2001.1.24 *T personal communication to FlyBase *u *F From struhl@cuccfa.ccc.columbia.edu Wed Jan 24 16:25:44 2001 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: UAS-al/AR143 *F Dear Rachel, *F Yes, I made this construct, and yes it is as Gerard described it with *F the >CD2,y+> flp-out cassette first described in the Jiang and Struhl, *F '95 and Zecca et al., 1995 papers. *F Best wishes. *F Gary *F 'Rachel Drysdale (Genetics)' wrote: *F > *F > Dear Gary, *F > *F > I enclose below a correspondence between myself and Gerard Campbell. I *F > am writing to you for confirmation that the flip-out cassette was *F > indeed the the CD2, y+ cassette (which I see discussed in Zecca et al., *F > 1995) that Gerard mentions \- if so I have all the info I need. *F > *F > With best wishes, *F > *F > Rachel. *F > *F > \---------------------------------------------------------------------- *F > Rachel Drysdale, Ph.D. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: rd120@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > *F > FlyBase: http://fly.ebi.ac.uk:7081/ *F > \---------------------------------------------------------------------- *F > *F > >From camp+@pitt.edu Wed Jan 24 13:33:02 2001 *F > Envelope-to: rd120@gen.cam.ac.uk *F > Delivery-date: Wed, 24 Jan 2001 13:33:02 \+0000 *F > Date: Wed, 24 Jan 2001 08:37:38 \-0500 *F > From: Gerard Campbell *F > Subject: Re: Helping FlyBase: UAS-al *F > To: 'Rachel Drysdale (Genetics)' *F > Reply-to: camp@pitt.edu *F > Organization: University of Pittsburgh *F > MIME-version: 1.0 *F > X-Mailer: Mozilla 4.7 (Macintosh; U; PPC) *F > Content-transfer-encoding: 7bit *F > X-Accept-Language: en,pdf *F > *F > Dear Rachel, *F > It was made by Gary Struhl \- as far as I can remember the original *F > construct is an al cDNA in pUAST with a flp out cassette between the UAS *F > sequence and the cDNA (although I am not absolutely sure it is pUAST, but *F > I don't think he uses anything else). The flp out cassette is y+ but is *F > probably the CD2, y+ cassette (original ref Jiang and Struhl 95 or Zecca *F > et al 95). Sorry I can't be more helpful, if this isn't good enough, you *F > could e-mail Gary \- I think his name for the construct is AR143. *F > Gerard *F > *F > 'Rachel Drysdale (Genetics)' wrote: *F > *F > > Dear Gerard, *F > > *F > > I am writing to you about a UAS-al construct that appears in *F > > \*x FBrf0131382 == Pueyo et al., 2000, Development 127(24): 5391--5402 *F > > with you stated to be the originator. *F > > *F > > We have no record of this construct in FlyBase and must conclude that *F > > it is new (i.e. has not yet been published) so I need to make a new *F > > transposon construct for it and what not. It would be best if the data *F > > I include in the records is accurate, Juan Pablo tells me it is a *F > > flipped out version of a bigger construct (with a y+ cassette?) but *F > > seems slightly unclear as to who made it. *F > > *F > > If you can fill me in on one or two details I would be most grateful. *F > > It is likely that we have records for related constructs (a y+ *F > > appears in P{&agr;Tub84B(FRT.y+)hh.B} from Basler and Struhl, 1994, *F > > Nature 368(6468): 208--214) so references to related publications would *F > > help me get it all correct. *F > > *F > > Hope all is going well with you, *F > > *F > > With best wishes, *F > > *F > > Rachel. *F > > *F > > \---------------------------------------------------------------------- *F > > Rachel Drysdale, Ph.D. *F > > *F > > FlyBase (Cambridge), *F > > Department of Genetics, *F > > University of Cambridge, *F > > Downing Street, email: rd120@gen.cam.ac.uk *F > > Cambridge, CB2 3EH, Ph : 01223-333963 *F > > UK. FAX: 01223-333992 *F > > *F > > FlyBase: http://fly.ebi.ac.uk:7081/ *F > > \---------------------------------------------------------------------- *F > *F > \-- *F > Gerard Campbell *F > Department of Biological Sciences *F > University of Pittsburgh *F > 215A Clapp Hall *F > Fifth Avenue at Ruskin *F > Pittsburgh, PA 15260 *F > *F > Tel: (412) 624 6812 *F > Fax: (412) 624 4759 *F \-- *F 212-305-3575 (phone); 212-740-2719 (fax) *F HHMI HHSC 1116, 701 W 168th St., NY, NY 10032 # *U FBrf0133295 *a Bloomington Drosophila Stock Center *b ?. *t 2001.1.27 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Sat Jan 27 18:03:42 2001 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Sat, 27 Jan 2001 18:03:42 \+0000 *F Date: Sat, 27 Jan 2001 12:55:31 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: more map data for GAL4 enhancer trap *F To: flybase-updates@morgan.harvard.edu *F MIME-Version: 1.0 *F Content-MD5: ulXdY9nsTIZOKWHiz5BS/g== *F X-Mailer: dtmail 1.3.0 CDE Version 1.3 SunOS 5.7 sun4m sparc *F Personal communication from: Kathy Matthews, Bloomington Drosophila *F Stock Center, Indiana University *F Subject: more map data for GAL4 enhancer traps *F Dated: 27 January 2001 *F Information communicated: *F The following enhancer-trap insertions described in FBrf0095642 *F have been mapped at the Bloomington Stock Center to the *F chromosomes indicated. *F Insertion Chromosome *F P{GawB}c315a 3 *F P{GawB}167Y 1 *F P{GawB}c306 1 *F P{GawB}198Y 4 *F P{GawB}c329b 3 *F P{GawB}c522 3 *F P{GawB}645b 3 *F P{GawB}c49 1 *F P{GawB}c355 1 # *U FBrf0133296 *a Pignoni *b F. *t 2001.1.26 *T personal communication to FlyBase *u *F From Francesca_Pignoni@meei.harvard.edu Fri Jan 26 19:53:37 2001 *F To: flybase-help@morgan.harvard.edu *F I wanted to inform you that sine oculis interacts genetically also with *F eyeless (ey2/ey2; so3/+ flies have more severely reduced eyes that ey2/ey2; *F \+/+ flies). regards, FP *F Francesca Pignoni, Ph.D. *F Dept. of Ophthalmology (Genetics) *F Harvard Medical School *F 243 Charles Street, MEEI 509 *F Boston, MA 02114 *F (617) 573-5552 (off.) *F (617) 573-3978 (lab) *F (617) 573-4290 (fax) # *U FBrf0133297 *a Laverty *b T. *t 2001.2.2 *T personal communication to FlyBase *u *F From tlaverty@uclink4.berkeley.edu Fri Feb 02 20:41:23 2001 *F To: Robert Levis , flybase-help@morgan.harvard.edu *F Subject: Re: Insertion site for l(3)L4111 *F Dear Bob, *F I made a booking keeping error in the cytology for l(3)L4111. The *F insertion site should be 68C1-4 NOT 66C1-4 that was reported before. *F Both the original slide and the image in BFD clearly show this. I *F made the changes on BFD already. Although this is not completely *F consistent with the flanking sequence (68B1), it is in the ballpark. *F I hope this helps you out. *F Take care, *F Todd *F At 2:39 PM \-0500 2/1/01, Robert Levis wrote: *F >There's a discrepancy in the insertion position of the P element *F >l(3)L4111 between the insertion position as determined by in situ *F >hybridization (66C1-4) and by flanking sequence (68B1). The *F >Berkeley Fly Database record shows an image of the in situ *F >hybridization. Todd, could you double check the reading of the in *F >situ? There is no genetic non-complementation data to verify that *F >the in situ localization is or is not consistent with the lethal *F >mutation. I think there is a stock for this in the Bloomington *F >Stock Center, but I can't connect at the moment to check it. *F >-- *F >------------------------------------ *F >Robert W. Levis, Ph.D. *F >Carnegie Institution of Washington *F >Department of Embryology *F >115 West University Parkway *F >Baltimore, MD 21210 *F > *F >phone: (410) 554-1238 *F >fax: (410) 467-1147 *F >email: levis@ciwemb.edu *F >------------------------------------ # *U FBrf0133298 *a Struhl *b G. *t 2001.2.5 *T personal communication to FlyBase *u *F From struhl@cuccfa.ccc.columbia.edu Mon Feb 05 14:00:49 2001 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: UAS-slp1 *F Dear Rachel, *F The original transgene is a UAS>CD2,y+>slp1. Manfred used a form of the *F transgene from which the >CD2,y+> was excised, so a residual FRT ('>') *F remains between the UASpromoter and slp1 sequences. The vector is *F pUAST. slp1 is a cDNA. *F Hope this is what you need. *F Best wishes. *F Gary *F 'Rachel Drysdale (Genetics)' wrote: *F > *F > Dear Gary, *F > *F > I am curating this paper of Manfred's for FlyBase: *F > *F > FBrf0131347 == Lee and Frasch, 2000, Development 127(24): 5497--5508 *F > *F > and have a question for you about the construct they refer to as *F > UAS-slp1 (insertion on third chromosome, 'gift from Gary Struhl'). We *F > have a record of a UAS-slp1 construct in FlyBase, FBtp0008246 == *F > P{UAS-slp1.R}, but it was published in *F > *F > FBrf0096157 == Riechmann et al., 1997, Development 124(15): 2915--2922 *F > *F > and there is no obvious relation to you in this reference. Manfred has *F > confirmed that this is not the same one as yours. So I need to make a *F > new transposon for your UAS-slp1. Is it simply a slp1 cDNA in pUAST? *F > *F > I will curate your reply as a personal communication to FlyBase, so *F > that this new transposon will be credited to you. *F > *F > All the best, *F > *F > Rachel. *F > *F > \---------------------------------------------------------------------- *F > Rachel Drysdale, Ph.D. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: rd120@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > *F > FlyBase: http://fly.ebi.ac.uk:7081/ *F > \---------------------------------------------------------------------- # *U FBrf0135153 *a DiAntonio *b A. *t 2001.2.1 *T personal communication to FlyBase *u *F Date: Wed, 21 Feb 2001 08:31:05 \-0600 *F To: Chihiro Yamada *F From: Aaron DiAntonio *F Subject: Re: FlyBase Query (cy1054/5) *F >Dear Chihiro, *F > *F >I made the elavGal4 lines you mentioned by mobilizing the elavGal4 *F >transgene that was generated by Luo. Luo fused the elav promoter to *F >the Gal4 gene. His insertion was on the second and was lethal. I *F >did a hop and selected for viable inserts on the 3rd. My lines were *F >not generated from C155 and so should not affect the endogenous elav *F >gene. I do not know where on the 3rd they have inserted. I *F >probably sent Rajagopalan the 3A insert. *F > *F > Yours, Aaron DiAntonio *F \-- *F \------------------------------------------------------------------------ *F Aaron DiAntonio, M.D., Ph.D. *F Assistant Professor *F Department of Molecular Biology and Pharmacology *F Washington University School of Medicine *F Campus Box 8103, 660 S. Euclid *F St. Louis, MO 63110 *F phone 314-362-9925 *F fax 314-362-7058 *F email dianton@molecool.wustl.edu *F http://pharmdec.wustl.edu/diantonio.html *F \------------------------------------------------------------------------ *F To: dianton@pcg.wustl.edu *F Subject: FlyBase Query (cy1054/5) *F From: Chihiro Yamada *F Date: Wed, 21 Feb 2001 11:32:28 \+0000 *F Dear Dr DiAntonio, *F I am currently curating two papers for FlyBase: *F Simpson et al., 2000, Neuron 28(3): 753--766 *F Rajagopalan et al., 2000, Neuron 28(3): 767--777 *F In these papers the authors name you as the source of elav-GAL4 lines. *F Simpson et al. state that they were made by mobilising the C155 *F elav-GAL4. and call them elav-GAL4 3A and 3E, while Rajagopalan only *F calls the line used elav-GAL4. *F We do not have these in our records \- have they been published before? *F If so what name was used to describe them. If they are new can you *F confirm that they were made by local hopping the P{GawB} element from *F C155 elav-GAL4? Do you have any more details about these lines? (e.g. *F are the new insertions within the elav gene? Do the insertions cause *F any mutant phenotype? ) Can you also tell me which line you sent to *F Rajagopalan et al.? *F Any new information will be curated as a personal communication from *F you to FlyBase if thats fine with you. *F Thanks and best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0135166 *a Yang *b X. *t 2001.4.17 *T personal communication to FlyBase *u *F Date: Mon, 16 Apr 2001 17:10:01 \+0800 *F To: Chihiro Yamada *F From: Yang Xiaohang *F Subject: Re: FlyBase Query (cy1076) *F Dear Chihiro *F We got the eve-tau-lacZ marker line from Chris Doe's lab (see the Materials *F and Methods in Wolf et al. (1998) Development, 125, 3853-3863). *F It is OK to quote my previous email as a personal communication to FlyBase. *F Sorry that I did not make these issues clear in the paper and hope that I *F have answered all your questions. *F Cheers *F Xiaohang *F At 11:46 AM 4/12/01 \+0100, you wrote: *F >Dear Xiaohang, *F > *F >Thanks for re-sending your reply, your original must have gone astray in *F >the electronic ether. *F > *F >I have another question I should have asked you the first time, In *F >figure 2 you show staining by and eve-tau-lacZ line for which you cite, *F >Fujioka et al., 1999, Development 126(11): 2527--2538. In this paper *F >there are a whole series of eve-lacZ line that are called in FlyBase: *F > *F >Ecol\lacZeve.F1 to Ecol\lacZeve.F40 *F > *F >Is the line you used one of these? *F > *F >Also is it OK for me to make your last email a personal communication *F >from you to FlyBase. The information you gave me about the 443/24 line *F >is potentially useful to users and not stated anywhere else. *F > *F >Thanks *F >Chihiro *F > *F > *F > > Dear Chihiro *F > > My apologies for not replying you earlier. I have to dig into my old *F > > notebooks dated sometime 1994 to answer your questions. Yes, I made a *F > > mistake and quoted wrongly in the paper the origin of the P1280 line. It *F > > was from the Keith collections (Hungarian lines) and the original *F > number is *F > > 443/24. You should be able to convert it to a P-line starting with the *F > > letter k (sorry I can not remember the rules for conversion now). The *F > > second question is easier for me. We used the Scer\GAL4elav-C155 *F > line in *F > > our experiment. *F > > Hope these answers are useful. *F > > Cheers *F > > Xiaohang *F > > *F > > >Dear Dr Yang, *F > > > *F > > >I am currently curating your paper for Flybase: *F > > > *F > > >Bahri et al., 2001, Mech. Dev. 100(2): 291--301 *F > > > *F > > >I have a couple of quick questions I was hoping you could answer for me. *F > > > *F > > >P1280 *F > > >----- *F > > >You describe a lacZ enhancer trap P insertion you call P1280 citing *F > > >Bloomington as your source. I cannot find a stock meeting this *F > > >description with the identifier P1280. Do you have any other *F > > >information about this line that might help me identify exactly which *F > > >insertion you used? Do you have a Berkeley identifier (a five figure *F > > >number possibly starting with a k, e.g.). Can you tell me which *F > > >transposon was used to make this line? (Was it a P{PZ} line or a *F > > >P{lacW} line). *F > > > *F > > > *F > > >elav-GAL4 *F > > >--------- *F > > >In your paper you mention an elav-GAL4 line. There are two these in *F > > >our records, could you tell me which one you used in your experiments? *F > > >If you are not sure could you give me any information you can so I can *F > > >work it out? For example, is it an enhancer trap line or a promoter *F > > >fusion? Where did get the line from? Do you know of any papers that *F > > >this line has been used in? *F > > > *F > > >Scer\GAL4elav.PLu *F > > > Luo et al., 1994, Genes Dev. 8(15): 1787--1802 *F > > > P{GAL4-elav.L} *F > > > GAL4 expression is driven by an @elav@ promoter. *F > > > *F > > >Scer\GAL4elav-C155 *F > > > Rebay and Rubin, 1995, Cell 81(6): 857--866 *F > > > P{GawB}elavC155 *F > > > Enhancer trap *F > > > *F > > > *F > > >Best wishes, *F > > > *F > > >Chihiro *F > > > *F > > >---------------------------------------------------------------------- *F > > >Chihiro Yamada. *F > > > *F > > >FlyBase (Cambridge), *F > > >Department of Genetics, *F > > >University of Cambridge, *F > > >Downing Street, email: c.yamada@gen.cam.ac.uk *F > > >Cambridge, CB2 3EH, Ph : 01223-333963 *F > > >UK. FAX: 01223-333992 *F > > >---------------------------------------------------------------------- *F > > > *F > > > *F > > >----- End Included Message \----- *F > > *F > > # *U FBrf0135167 *a Scully *b A. *t 2001.3.30 *T personal communication to FlyBase *u FlyBase error report for CG14751. *F From FlyBase-error@hedgehog.lbl.gov Thu Jan 25 02:08:47 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 25 Jan 2001 02:08:47 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 24 Jan 2001 18:08:42 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: audras2@usa.net *F Subject: FlyBase error report for CG14751 on Wed Jan 24 18:08:42 2001 *F Content-Length: 1480 *F Error report from Dr. Audra Scully (audras2@usa.net) *F Gene or accession: CG14751 *F Gene annotation error *F Genes CG14751 and CG8643 should be merged. *F Comments: I've sequenced the entire EST GH19651 and the cDNA encompasses both *F these 'genes'. My EST consensus sequence matches the genomic except in two *F places a 'g' has been substituted for 'a' both making amino acid changes. *F These two 'g's' are present also in sequence posted for an overlapping EST *F (GH06282) .. *F From audras2@usa.net Mon Feb 12 20:49:10 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Feb 2001 20:49:10 \+0000 *F Date: 12 Feb 2001 12:49:32 PST *F From: Audra Scully *F To: Gillian Millburn (Genetics) *F Subject: Re: Re: FlyBase error report for CG14751 on Wed Jan 24 18:08:42 2001 *F X-Mailer: USANET web-mailer (34FM.0700.15B.01) *F Mime-Version: 1.0 *F Content-Transfer-Encoding: quoted-printable *F Content-Type: text/plain; charset='US-ASCII' *F Content-Length: 4237 *F Dear Gillian, *F Here's an update. It appears that the EST GH27921 is a different splice *F variant compared to GH19651, and the area that is present in GH19651 that *F causes the two coding frames to be out of frame, is removed in GH27921. So in *F effect, GH27921 codes for both of those CG's as one protein. SD02665 and *F GH06282 appear to have other splice variations as well but I don't have all *F the sequence on those. I'll submit the sequence for GH27921 and GH19651 to *F genebank in the next month or so since I'm publishing a paper on this gene in *F the next few months. Thanks, Audra *F .. *F From audras2@usa.net Tue Feb 13 17:45:12 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 13 Feb 2001 17:45:12 \+0000 *F Date: 13 Feb 2001 09:45:13 PST *F From: Audra Scully *F To: Gillian Millburn (Genetics) *F Subject: update for FlyBase error report for CG14751 *F X-Mailer: USANET web-mailer (34FM.0700.15B.01) *F Mime-Version: 1.0 *F Content-Transfer-Encoding: quoted-printable *F Content-Type: text/plain; charset='US-ASCII' *F Content-Length: 1793 *F Dear Gillian, *F ..The bottom line now is that EST GH19651 is likely an incompletely processed *F transcript containing a small intron that disrupts the reading frame, while *F EST GH27921 has that intron spliced out. .. CG14751 and CG8643 .. do indeed *F code for one *F large C2H2 zinc finger protein from one transcript. I'm sequencing GH27921 in *F its entirety to verify the rest of the sequence with what I got for GH19651. *F At least now they'll know that CG14751 and CG8643 are one transcript and one *F continuous protein (minus the first MQ of CG6843). Once I have the sequence *F verified for GH27921 (in a couple of weeks), I'll submit the entire DNA and *F protein sequence to Genbank. *F Hope that helps, Audra *F .. *F From audras2@usa.net Wed Mar 21 18:12:22 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 21 Mar 2001 18:12:22 \+0000 *F Date: 21 Mar 2001 10:12:24 PST *F From: Audra Scully *F To: Gillian Millburn (Genetics) *F Subject: Re:update for FlyBase error report for CG14751 *F X-Mailer: USANET web-mailer (34FM.0700.16A.01) *F Mime-Version: 1.0 *F Content-Transfer-Encoding: quoted-printable *F Content-Type: text/plain; charset='US-ASCII' *F Content-Length: 1171 *F Hi Gillian, *F ..I have submitted the sequence to *F Genbank for the gene regular (rgr) corresponding to the locus that the EST *F GH27921 represents. That Genbank \# is AF353512 and is scheduled for release *F in June. From my other e-mail account (which handles attachments better) I'll *F send you the protein and DNA sequence for GH27921 I submitted to Genbank as *F well as the DNA sequence for GH19651 to help Flybase clarify this region. *F Those attachments will be in Word. I hope this helps, Audra *F .. *F From ascully@acadia-pharm.com Wed Mar 21 18:18:45 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 21 Mar 2001 18:18:45 \+0000 *F From: Audra Scully *F To: ''gm119@gen.cam.ac.uk'' *F Subject: files attached *F Date: Wed, 21 Mar 2001 10:16:20 \-0800 *F MIME-Version: 1.0 *F X-Mailer: Internet Mail Service (5.5.2653.19) *F Content-Type: multipart/mixed; boundary='----_=_NextPart_000_01C0B233.07C7E170' *F Content-Length: 95811 *F Dear Gillian, *F Here are the sequence files for the regular locus. Audra <> <> <> *F \------------------------------------------------------------------------------ *F -- *F <> *F MMPLQESPSPSWQLEDYAFFRKCGEITVSPDVQSFGFNCAFCPAICLQFSVFMDHIRVQHTQDVSRRYETEEERCPQTD *F LVIMELDCPEIYPPEAPRKCSWEADMDIQLMSLVPSPPAVHIAPLPPPKTTETPEIDLVYVVENPLPPSPPPSADISVD *F ELPGSSPQSFDLDPFAVLHEVVTMTPPTPIAPPPGPPTPNPTPTATPAPLPRYETRRVAMQRKLRQSQNEQPKDGAVDE *F VAVEEHKGTEPTGQPEKSAMELTPPATPPTPLPPTSPTSSTASCADFQSKSRRELERNHEFVGCLLREYERTEKLWNPR *F HPDYKYNAKRSAYGDLAGPLESICHRQLSGAEIFAVLKELRCRYRRELKKVNALGGKYKSRLWYFERMDFLRCVIENRR *F AEREAKISNESTESEKSCETDADSCGKSSLYHEVLSFILDAFKRQECLWNPQHYDYTTCCKTELFRDISVQLQEELNYE *F LSGEECCNEIQKLRTRYRKELRMVIKHKGLYLPKLWCYDEMEFLQPILQEQIFNKISKKIGVVGSNQKTKFIDASSIRF *F DNTEKQLQFVEIYHNCSALWDVDHPDFRSNTYRSQALGQMLDEINTTFHTSYTAERLEKTLFNLRKEFSAQKRKILTES *F EDSSSIPLLHAKLAEFLDQNLGPFRCDICSDLVKTCDQYKVHRSAHDGTQPFICTLCGKGFQMPCNLTVHIRRHRRDFP *F YSCEQCDKRFATSTEVAIHLRTHTGERPYICDLCGKSFKTWSFFDIHRRRHLNQSTFHCPICAKGFYEKNRFTDHMNSH *F WAIRKHLCTVCGKTFTTYGNLKKHTELHLAVKKYKCGTCGKRFAQFASLRWHKKREHSSVGQAGGK *F \------------------------------------------------------------------------------ *F -- *F <> *F cagcggcgttggttcggaaaagtgcagtctccgcgtcgcgagtgcgccaataacgagaaataacgagaattgaaaaagt *F tccttaagaggtggagtccagtaaagcgcagcagcaatgatgccgctgcaggagtcgccgtcgcccagttggcagctgg *F aggactacgctttcttccgcaagtgcggggaaatcaccgtctcgccggacgtgcagagcttcggcttcaactgcgcctt *F ctgtccggcgatctgcctgcagttctccgtgttcatggaccacatccgggtgcagcacacgcaggacgtgagccggcgc *F tacgagactgaggaggagaggtgtccgcaaaccgatctggtcatcatggaactcgactgccccgagatttatccgccgg *F aggcgcctaggaaatgcagctgggaggccgacatggacatccagctaatgagcctcgttccgtcgcctcccgcagtgca *F catagcacctctaccaccacctaaaacgaccgaaacaccagaaatagatttggtctatgtggttgaaaatccattacca *F ccctcgccgccgccctccgcggacatttcggtcgacgagctgccgggaagcagtccgcagtccttcgacctggatccct *F ttgccgtgctgcacgaggtagtaacgatgactccgcccactcccattgctccaccaccgggcccgccgacccccaatcc *F gactccaactgcgactccggccccattgccgcggtacgagacacgacgcgtggcaatgcagcgcaagcttcgtcagtcg *F caaaacgagcaacctaaagatggggcggtggacgaggtcgcggtggaggagcacaagggaacggaacccactggacagc *F cggagaagagtgctatggaactaacaccacctgccacgccgcccaccccactgccgcccacctcgcccacgagcagcac *F ggcaagctgtgcggatttccaatcgaaaagtcgcagggagctagaacgtaatcacgagttcgttggctgtctgctgagg *F gagtacgagcgcacggaaaagctgtggaatccccggcatccggactacaagtacaatgccaagcgcagtgcctacggtg *F atctggccggtccgctggagtccatttgccacaggcagctctccggagccgagatcttcgctgtgctaaaggagttgag *F gtgcagataccgccgcgagctgaaaaaggtgaacgccctgggtggaaagtacaagtcgcgtctgtggtactttgagagg *F atggactttctgcggtgtgtcatcgaaaacaggcgagccgaaagggaagccaagatttccaatgagagcacagagagtg *F aaaagtcgtgcgaaacggacgctgattcctgcggcaagtcatctttgtaccacgaggttctgagtttcattctggatgc *F cttcaagaggcaggaatgcctgtggaatccgcagcactacgactacaccacatgctgtaagacggaactgtttcgcgac *F atctccgtccagttgcaggaggagctcaactatgagctgagcggcgaggaatgctgcaacgagatccaaaaacttagga *F cacgttaccgcaaggagctgcgcatggttattaagcacaagggattgtacctacccaagttatggtgttacgatgaaat *F ggagttcctgcagcccatactgcaggagcagatcttcaacaagatcagcaagaaaatcggagtggtgggaagcaaccag *F aagaccaagttcatcgatgccagctcaattcgttttgacaatactgagaaacaactgcagtttgtagagatttaccaca *F actactcagctctgtgggatgttgaccatcccgacttccgatcgaatacgtatcgtagtcaggctttgggtcaaatgtt *F ggatgagataaacacaacctttcacacttcctacactgcggagcagttggaaaagaccttgttcaatctgcgcaaagaa *F ttctccgcccagaaacggaagatacttacggagtccgaagactccagcagcattccgctgctgcatgccaaactggcag *F agttcctagaccaaaatcttggtccttttcgttgcgatatatgctcggacctggtcaagacctgcgatcagtacaaggt *F gcatcgatccgcacacgatggcacccaacccttcatctgcactc *F \------------------------------------------------------------------------------ *F -- *F <> *F gccggttgtttggatcgcagattgaatcggaaagccagcggcgttggttcggaaaagtgcagtctccgcgtcgcgagtg *F cgccaataacgagaaataacgagaattgaaaaagttccttaagaggtggagtccagtaaagcgcagcagcaatgatgcc *F gctgcaggagtcgccgtcgcccagttggcagctggaggactacgctttcttccgcaagtgcggggaaatcaccgtctcg *F ccggacgtgcagagcttcggcttcaactgcgccttctgtccggcgatctgcctgcagttctccgtgttcatggaccaca *F tccgggtgcagcacacgcaggacgtgagccggcgctacgagactgaggaggagaggtgtccgcaaaccgatctggtcat *F catggaactcgactgccccgagatttatccgccggaggcgcctaggaaatgcagctgggaggccgacatggacatccag *F ctaatgagcctcgttccgtcgcctcccgcagtgcacatagcacctctaccaccacctaaaacgaccgaaacaccagaaa *F tagatttggtctatgtggttgaaaatccattaccaccctcgccgccgccctccgcggacatttcggtcgacgagctgcc *F gggaagcagtccgcagtccttcgacctggatccctttgccgtgctgcacgaggtagtaacgatgactccgcccactccc *F attgctccaccaccgggcccgccgacccccaatccgactccaactgcgactccggccccattgccgcggtacgagacac *F gacgcgtgcgccagcgattgtgtacgatttccagcaccacgataaaccccggacaagatcgtctggctgggcagcgctt *F atctcgcagagagcccaacactggcaacgttacaaatttatttatttatgcgaaatatttatacttactctatactcta *F tgcaggcaatgcagcgcaagcttcgtcagtcgcaaaacgagcaacctaaagatggggcggtggacgaggtcgcggtgga *F ggagcacaagggaacggaacccactggacagccggagaagagtgctatggaactaacaccacctgccacgccgcccacc *F ccactgccgcccacctcgcccacgagcagcacggcaagctgtgcggatttccaatcgaaaagtcgcagggagctagaac *F gtaatcacgagttcgttggctgtctgctgagggagtacgagcgcacggaaaagctgtggaatccccggcatccggacta *F caagtacaatgccaagcgcagtgcctacggtgatctggccggtccgctggagtccatttgccacaggcagctctccgga *F gccgagatcttcgctgtgctaaaggagttgaggtgcagataccgccgcgagctgaaaaaggtgaacgccctgggtggaa *F agtacaagtcgcgtctgtggtactttgagaggatggactttctgcggtgtgtcatcgaaaacaggcgagccgaaaggga *F agccaagatttccaatgagagcacagagagtgaaaagtcgtgcgaaacggacgctgattcctgcggcaagtcatctttg *F taccacgaggttctgagtttcattctggatgccttcaagaggcaggaatgcctgtggaatccgcagcactacgactaca *F ccacatgctgtaagacggaactgtttcgcgacatctccgtccagttgcaggaggagctcaactatgagctgagcggcga *F ggaatgctgcaacgagatccaaaaacttaggacacgttaccgcaaggagctgcgcatggttattaagcacaagggattg *F tacctacccaagttatggtgttacgatgaaatggagttcctgcagcccatactgcaggagcagatcttcaacaagatca *F gcaagaaaatcggagtggtgggaagcaaccagaagaccaagttcatcgatgccagctcaattcgttttgacaatactga *F gaaacaactgcagtttgtagagatttaccacaactgctcagctctgtgggatgttgaccatcccgacttccgatcgaat *F acgtatcgtagtcaggctttgggtcaaatgttggatgagataaacacaacctttcacacttcctacactgcggagcggt *F tggaaaagaccttgttcaatctgcgcaaagaattctccgcccag *F \------------------------------------------------------------------------------ *F -- # *U FBrf0135184 *a Dickson *b B. *t 2001.2.7 *T personal communication to FlyBase *u *F From dickson@nt.imp.univie.ac.at Wed Feb 07 11:59:01 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 11:59:01 \+0000 *F Mime-Version: 1.0 *F Date: Wed, 7 Feb 2001 13:03:58 \+0100 *F To: Gillian Millburn (Genetics) *F From: Barry Dickson *F Subject: Re: FlyBase query *F Dear Gillian, *F This is one we made ourselves quite some time ago. It is Chris Callahan and *F John Thomas's tau-lacZ (which they provided in pBluescript) subcloned into *F Andrea Brand's pUAST. It is probably identical (except maybe restriction *F sites) to the construct others are using. *F Best wishes, *F Barry *F >Dear Barry, *F > *F > *F >I have another question about your paper: *F > *F >Rajagopalan et al., 2000, Cell 103(7): 1033-1045 *F > *F >In Figure 5 you use a 'UAS-taulacZ' construct. *F > *F >Could you tell me who you got it from or a reference where it has been *F >used, as there are 2 very similar UAS-tau-lacZ constructs in FlyBase *F >and I need to figure out which one it is (if you got it from *F >Bloomington can you tell me the stock number), *F > *F >that's it \! *F > *F >Gillian *F \-------------------------------------------------- *F Barry Dickson *F Research Institute of Molecular Pathology (I.M.P.) *F Dr. Bohr-Gasse 7 *F A-1030 Vienna *F Austria *F Tel. \+43 1 797 30 421 *F Fax. \+43 1 798 71 53 *F Email. dickson@nt.imp.univie.ac.at *F \-------------------------------------------------- # *U FBrf0135185 *a Pirrotta *b V. *t 2001.2.28 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Feb 07 17:49:51 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 17:49:51 \+0000 *F To: vincenzo.pirrotta@zoo.unige.ch *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 7 Feb 2001 17:49:53 \+0000 *F Content-Length: 1145 *F Dear Dr. Pirrotta, *F I am curating your paper for FlyBase: *F Poux et al., 2001, Development 128(1): 75--85 *F and I have a question. *F 1. .. 'PBX+2212H1' transposon in Figure 7C. *F You reference the paper: *F Poux et al., 1996, EMBO J. 15(17): 4713--4722 *F for this construct. I would be grateful if you could tell me the name *F of the transposon in that paper, as FlyBase has a record of several *F constructs from that paper, and I need to work out which one it is, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From pirrotta@zoo.unige.ch Wed Feb 28 14:34:24 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 28 Feb 2001 14:34:24 \+0000 *F Date: Wed, 28 Feb 2001 14:56:55 \+0100 *F From: 'V. Pirrotta' *F Subject: Re: FlyBase query *F To: 'Gillian Millburn (Genetics)' *F MIME-version: 1.0 *F X-Mailer: Microsoft Outlook Express Macintosh Edition \- 4.5 (0410) *F Content-transfer-encoding: 7bit *F X-Priority: 3 *F Dear Ms Millburn *F .. *F The PBX+2212H1 transposon is not explicitly listed in the Poux et al. 1996 *F paper. It contains the same PBX and 2212H1 fragments that are used in the *F constructs in Table II of that paper but without the PRE fragment. The *F origin of the fragments is shown in Figure 1 of that paper. If you prefer, I *F can look up the exact positions defining these fragments in the published *F sequence of the Ubx gene. *F Prof. Vincenzo Pirrotta *F Department of Zoology *F University of Geneva *F 30 quai Ernest Ansermet *F CH1211 Geneva, Switzerland *F Tel. : (41 22) 702 6786 *F FAX : (41 22) 702 6776 *F Email: pirrotta@zoo.unige.ch # *U FBrf0135186 *a Whitfield *b E. *t 2001.2.5 *T personal communication to FlyBase *u FlyBase error report for CG10852 on Mon Feb 5 03:09:14 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Feb 05 11:09:22 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 5 Feb 2001 11:09:22 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 5 Feb 2001 03:09:14 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10852 on Mon Feb 5 03:09:14 2001 *F Content-Length: 533 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10852 *F Gene annotation error *F Gene CG10852 has incorrect exon/intron structure. *F Comments: Acp63F (Celera translation AE003479; AAF47797) is missing the first *F exon by *F comparison to numerous strains sequenced by Begun et al, Genetics 156:1879-1888 *F (2000), AY010618-AY010626. *F Exon is there and translation is 100% sequence match to that from AY010618- *F AY010626. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135187 *a Whitfield *b E. *t 2001.2.28 *T personal communication to FlyBase *u FlyBase error report for CG11579 on Wed Feb 28 01:31:57 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 28 09:32:02 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 28 Feb 2001 09:32:02 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 28 Feb 2001 01:31:57 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11579 on Wed Feb 28 01:31:57 2001 *F Content-Length: 819 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11579 *F Release: 2 *F Gene annotation error *F Gene CG11579 has incorrect exon/intron structure. *F Comments: Celera translation of arm (AE003422; AAF45688) is short by 3 aa at *F the N- *F terminus. The mRNA feature includes the full translation but the CDS feature *F needs to be extended to cover the upstream 9 bases for the correct translation. *F Please compare to Riggleman et al, Genes Dev. 3:96-113(1989), X54468; CAA38350. *F AAF45688 \---MPAQNRTMSHNNQYNPPDLPPMVSAKEQTLMWQQNSYLGDSGIHSGAVTQVPSLSGK *F CAA38350 MSYMPAQNRTMSHNNQYNPPDLPPMVSAKEQTLMWQQNSYLGDSGIHSGAVTQVPSLSGK *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135188 *a Levis *b R. *t 2001.2.27 *T personal communication to FlyBase *u FlyBase error report for CG7437 on Tue Feb 27 10:25:16 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Feb 27 18:25:51 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 27 Feb 2001 18:25:51 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 27 Feb 2001 10:25:16 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG7437 on Tue Feb 27 10:25:16 2001 *F Content-Length: 985 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG7437 *F Release: 2 *F Gene annotation error *F Gene CG7437 has incorrect exon/intron structure. *F Comments: The present GenBank annotation for the scaffold segment, AE003596.2, *F places the 5' end of the mub mRNA (CT122869) at position 32,648. However, *F cDNA analyses show that the 5' end actually maps 27 kb upstream of this. A *F published cDNA analysis (Grams & Korge (1998) Gene 215: 191-201; sequence *F accession X99340) maps the 5' end at AE003596.2 position 4930 and reports a 5' *F exon corresponding to AE003596.2 4930-5238. Furthermore, the BDGP EST LD32520 *F for the mub gene has a similar 5' exon which extends slightly farther *F upstream, AE003596.2 4926-5238. Two P elements in the BFD are inserted in *F this 5' exon, EP(3)3623 and EP(3)3108. The P element l(3)00386 is inserted 54 *F nt upstream of this 5' exon. *F Browser: Mozilla/4.0 (compatible; MSIE 4.5; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135189 *a Gloor *b G. *t 2001.2.27 *T personal communication to FlyBase *u FlyBase error report for CG8674 on Tue Feb 27 09:23:14 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Feb 27 17:23:23 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 27 Feb 2001 17:23:23 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 27 Feb 2001 09:23:14 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ggloor@uwo.ca *F Subject: FlyBase error report for CG8674 on Tue Feb 27 09:23:14 2001 *F Content-Length: 2853 *F Error report from Greg Gloor (ggloor@uwo.ca) *F Gene or accession: CG8674 *F Release: 1 *F Missed gene *F Comments: CG8674 is similar to yeast gene YJL180C (gene name ATP12). *F Smith-Waterman searching revealed that these two genes are each other's best *F match in the fly and yeast proteomes. They share a 23% identity in an *F overlapping region extending the length of each sequence. PSI-BLAST with *F either YJL180C (BL62 or BL45) or CG8674 (BL45) identify the same set of six *F sequences from S. cerevisiae, S. pombe, C. elegans, D. melanogaster, A. *F thaliana. The SW alignment that triggered this investigation is below: *F (BLOSUM50 \-12 gap initiation, \-2 gap extension) *F >ORFP:YJL180C ATP12, Chr X from 87581-88558, reverse complement *F (325 aa) *F s-w opt: 146 *F Smith-Waterman score: 146; 22.930% identity (27.799% ungapped) in 314 aa *F overlap (3-262:8-320) *F 10 20 30 40 50 *F 60 *F CG8674 *F MNGKYVVSAIRALRLTNF----SQCKGAASSFT------VRHYASPPKRFYKKTSVLSG-DSG-YEVVLDHRKLK *F : ..:..:: :.: : .: :. ... . . .. ..:..:.:. ..: *F . :: : .: *F ORFP:Y *F MLPSLRKGCFIVNSIR-LKLPRFYSLNAQPLGTDNTIENNTPTETNRLSKTSQKFWEKVSLNRDVEKGKIALQLDGRTIK *F 10 20 30 40 50 60 70 *F 70 80 90 100 110 *F 120 *F CG8674 *F TPKGTPFIVRSEP--LAIAVATEFDA-QKENIERSRMHLSALCFTAID---------NPNHLSKL----DMV-NYLLNFI *F :: :. .:: . :: . :... .. .:. . :..: :: .:. ..:. *F :.. : :: .. *F ORFP:Y *F TPLGNGIIVDNAKSLLAYLLKLEWSSLSSLSIKTHSLPLTSLVARCIDLQMTNEPGCDPQLVAKIGGNSDVIKNQLLRYL *F 80 90 100 110 120 130 140 150 *F 130 140 150 160 170 180 *F 190 *F CG8674 *F ATDTVL-FQYDDEKD--LQDLQVNEWDPVIAW---FNQRYDTNLQKTMNITPPQV-----SEQDKM--NVAKHFQSY--- *F :::.: :. .: . :.. : . . :.: : . .... . ..: .. ..:. . *F :.::...: *F ORFP:Y *F DTDTLLVFSPMNEFEGRLRNAQNELYIPIIKGMEEFLRNFSSESNIRLQILDADIHGLRGNQQSDIVKNAAKKYMSSLSP *F 160 170 180 190 200 210 220 230 *F 200 210 220 230 240 250 *F 260 *F CG8674 *F \--------SLETLHGFIFAVDTLKSIVLACAVIEQMLT-VEKAVALARLEEEYQLKFWGRVEWAHDLSQQELQARLAA *F AV *F .. : ..:: .: :.. . .: . : ... : : :: .:.. ::.:: *F .::....... .. .:. *F ORFP:Y *F WDLAILEKTVLTTKSFICGVLLLENKKDTANLIPALKTDMDNIVRAATLETIFQVEKWGEVEDTHDVDKRDIRRKIHTAA *F 240 250 260 270 280 290 300 310 *F 270 *F CG8674 LFVHLNCSENLVKQKIIL *F . *F ORFP:Y IAAFKQ *F 320 *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135190 *a Whitfield *b E. *t 2001.2.27 *T personal communication to FlyBase *u FlyBase error report for CG6571 on Tue Feb 27 06:04:44 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Feb 27 14:04:50 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 27 Feb 2001 14:04:50 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 27 Feb 2001 06:04:44 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6571 on Tue Feb 27 06:04:44 2001 *F Content-Length: 435 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6571 *F Release: 2 *F Gene annotation error *F Gene CG6571 has incorrect exon/intron structure. *F Comments: Celera annotation of rdgC (AE003514; AAF49044) is missing the *F N-terminal coding *F exon. Please compare to Steele et al, Cell 69:669-676(1992) (M89628; AAB00734). *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135191 *a Porzgen *b P. *t 2001.2.22 *T personal communication to FlyBase *u FlyBase error report for CG8380 on Thu Feb 22 20:17:09 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Feb 23 04:17:53 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 23 Feb 2001 04:17:53 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Feb 2001 20:17:09 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: Poerzgen@ohsu.edu *F Subject: FlyBase error report for CG8380 on Thu Feb 22 20:17:09 2001 *F Content-Length: 1252 *F Error report from Peter Pörzgen (Poerzgen@ohsu.edu) *F Gene or accession: CG8380 *F Release: 1 *F Gene annotation error *F Gene CG8380 has incorrect exon/intron structure. *F Protein sequence: *F MSPTGHISKSKTPTPHDNDNNSISDERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLVVGGIP *F LFYMELALGQHNRKGAITCWGRLVPLFKGIGYAVVLIAFYVDFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCR *F PFESQNASRVPVIGNYSDLYAMGNQSLLYNETYMNGSSLDTSAVGHVEGFQSAASEYFNRYILELNRSEGIHDLGAIKW *F DMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQ *F VFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPA *F AIATMPASTFWALIFFMMLLTLGLDSSFGGSEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLL *F DRYAAGYSILVAVFFEAIAVSWIYGTNRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLFITVYGLIGYEPLTYADYVYP *F SWANALGWCIAGSSVVMIPAVAIFKLLSTPGSLRQRFTILTTPWRDQQSMAMVLNGVTTEVTVVRLTDTETAKEPVDV *F Comments: We have cloned the respective transporter cDNA and found an *F additional exon within the large extracellular loop: *F FESQNASRVPVIGNYSDLYAMGNQSLLYNETYMNGSSLDTSAVGHVEGFQSAASEYFN *F this translation can be predicted from your genomic sequence and is what we *F have found by cloning *F and describe in Mol.Pharmacol. 2001, 59, 83-95 *F cheers Peter *F Browser: Mozilla/4.08 (Macintosh; I; PPC, Nav) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135192 *a Whitfield *b E. *t 2001.2.21 *T personal communication to FlyBase *u FlyBase error report for CG1977 on Wed Feb 21 01:57:22 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 21 09:57:27 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 21 Feb 2001 09:57:27 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 21 Feb 2001 01:57:22 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG1977 on Wed Feb 21 01:57:22 2001 *F Content-Length: 891 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1977 *F Release: 2 *F Gene annotation error *F Gene CG1977 has a mistake in the supporting evidence or functional assignment. *F Comments: alpha-Spec is shown by Celera to have 2 isoforms. 1 isoform, CT6173, *F is a very *F good match to existing sequences for alpha-Spec. The second annotated isoform, *F CT41026, seems to share a non-coding exon but not a coding exon with CT6173. *F The sequences are very different and the translation from CT41026 shows no *F similarity to existing alpha-Spec sequences. In fact it shows no similarity to *F any protein in Swiss-prot or TrEMBL. *F I believe CT41026 should not be annotated as an isoform of alpha-Spec, also *F there is no other evidence from literature that alpha-Spec has splice variants. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135193 *a Whitfield *b E. *t 2001.2.19 *T personal communication to FlyBase *u FlyBase error report for CG3220 on Mon Feb 19 03:25:52 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Feb 19 11:25:59 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Feb 2001 11:25:59 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 19 Feb 2001 03:25:52 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3220 on Mon Feb 19 03:25:52 2001 *F Content-Length: 440 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3220 *F Release: 2 *F Gene annotation error *F Gene CG3220 has incorrect exon/intron structure. *F Comments: Celera annotation for Mlp60A (AE003462; AAF47158) defines an excess *F of exons. *F Please compare to Stronach et al, J. Cell Biol. 134:1179-1195(1996), X91244; *F CAA62626. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135194 *a Whitfield *b E. *t 2001.2.19 *T personal communication to FlyBase *u FlyBase error report for CG2096 on Mon Feb 19 03:22:12 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Feb 19 11:22:19 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Feb 2001 11:22:19 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 19 Feb 2001 03:22:12 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG2096 on Mon Feb 19 03:22:12 2001 *F Content-Length: 447 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG2096 *F Release: 2 *F Gene annotation error *F Gene CG2096 has incorrect exon/intron structure. *F Comments: Celera annotation for flw (AE003450; AAF46583) is missing the *F N-terminal coding *F exon. Please compare to Raghaven et al, Curr. Biol. 10:269-272(2000), *F AJ249214; CAB59732. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135195 *a Whitfield *b E. *t 2001.2.19 *T personal communication to FlyBase *u FlyBase error report for CG17947 on Mon Feb 19 03:09:59 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Feb 19 11:10:06 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Feb 2001 11:10:06 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 19 Feb 2001 03:09:59 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG17947 on Mon Feb 19 03:09:59 2001 *F Content-Length: 466 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG17947 *F Release: 2 *F Gene annotation error *F Gene CG17947 has incorrect exon/intron structure. *F Comments: Celera annotation for alpha-Cat (AE002656; AAF45466) is missing *F annotation of *F the N-terminal coding exon. Please compare to Oda et al, J. Cell Biol. 121: *F 1133-1140(1993), D13964; BAA03067. *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135196 *a Axelsen *b K. *t 2001.2.13 *T personal communication to FlyBase *u FlyBase error report for CG12348 on Mon Feb 12 23:26:21 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Feb 13 07:26:31 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 13 Feb 2001 07:26:31 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 12 Feb 2001 23:26:21 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: kristian.axelsen@isb-sib.ch *F Subject: FlyBase error report for CG12348 on Mon Feb 12 23:26:21 2001 *F Content-Length: 18117 *F Error report from Kristian Axelsen (kristian.axelsen@isb-sib.ch) *F Gene or accession: CG12348 *F Release: 2 *F DNA error at position 98153. Upstream nucleotides: tttatttcttcactaaatacc *F Insertion of length 1: c. *F Corrected sequence: tttatttcttcactaaatacgtctaaa. *F Comments: I have for SWISS-PROT looked at the Shaker sequence. The insertion *F is found by *F comparing to M17211. The CDS (and mRNA) is, furthermore, badly predicted. *F I here show a BLAST of M17211 against the genome: Note that (at least) 5 *F other isoforms exist *F >emb|AE003507|AE003507 Drosophila melanogasterDrosophila melanogaster *F genomic scaffold 142000013386053 section 24 of 30, *F complete sequence. *F Length = 297308 *F Score = 278 bits (140), Expect = 2e-72 *F Identities = 140/140 (100%) *F Strand = Plus / Minus *F Query: 8 ggagtttctatccagacttcaatatttttttacctcgctcaaaaccccccactcgcactt 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 135947 ggagtttctatccagacttcaatatttttttacctcgctcaaaaccccccactcgcactt *F 135888 *F Query: 68 taaataataaaaaaaagcaggtggtgcgtgccgcgtagccgcgcgtgattcttgttgttg 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 135887 taaataataaaaaaaagcaggtggtgcgtgccgcgtagccgcgcgtgattcttgttgttg *F 135828 *F Query: 128 ttttttttttttcggtgaat 147 *F |||||||||||||||||||| *F Sbjct: 135827 ttttttttttttcggtgaat 135808 *F Score = 878 bits (443), Expect = 0.0 *F Identities = 443/443 (100%) *F Strand = Plus / Minus *F Query: 148 ctcttgtaaccatgtaccaaagttctttgccgcgaaaactaaaatgaaaacgaaagtgaa 207 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 122264 ctcttgtaaccatgtaccaaagttctttgccgcgaaaactaaaatgaaaacgaaagtgaa *F 122205 *F Query: 208 aatgagcgaatggcagccgcggccacagcaatcgatccatgacacaaccagtgacaagca 267 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 122204 aatgagcgaatggcagccgcggccacagcaatcgatccatgacacaaccagtgacaagca *F 122145 *F Query: 268 gtcccccagtgaaaccgcatccgcatccgagtccgataccgataaagattctgaatcgga 327 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 122144 gtcccccagtgaaaccgcatccgcatccgagtccgataccgataaagattctgaatcgga *F 122085 *F Query: 328 gtgagtgccgcgtccgagagcgttccctgtccacgtccaccatcggcggagcaggtgtgc 387 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 122084 gtgagtgccgcgtccgagagcgttccctgtccacgtccaccatcggcggagcaggtgtgc *F 122025 *F Query: 388 ctgaggcccacctggtggcatggccgccgttgccggcctctatggccttggggaggatcg 447 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 122024 ctgaggcccacctggtggcatggccgccgttgccggcctctatggccttggggaggatcg *F 121965 *F Query: 448 ccagcaccgcaagaagcagcagcaacagcagcagcaccagaaggagcagctcgagcagaa 507 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121964 ccagcaccgcaagaagcagcagcaacagcagcagcaccagaaggagcagctcgagcagaa *F 121905 *F Query: 508 ggaggagcaaaagaagatcgccgagcggaagctgcagctgcgggagcagcagctccagcg 567 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121904 ggaggagcaaaagaagatcgccgagcggaagctgcagctgcgggagcagcagctccagcg *F 121845 *F Query: 568 caactccctcgatggttacgggt 590 *F ||||||||||||||||||||||| *F Sbjct: 121844 caactccctcgatggttacgggt 121822 *F Score = 252 bits (127), Expect = 1e-64 *F Identities = 127/127 (100%) *F Strand = Plus / Minus *F Query: 588 ggtctttgcccaaattgagcagtcaagacgaagaagggggggctggtcatggctttggtg 647 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 98766 ggtctttgcccaaattgagcagtcaagacgaagaagggggggctggtcatggctttggtg 98707 *F Query: 648 gcggaccgcaacactttgaacccattcctcacgatcatgatttctgcgaaagagtcgtta 707 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 98706 gcggaccgcaacactttgaacccattcctcacgatcatgatttctgcgaaagagtcgtta 98647 *F Query: 708 taaatgt 714 *F ||||||| *F Sbjct: 98646 taaatgt 98640 *F Score = 339 bits (171), Expect = 7e-91 *F Identities = 171/171 (100%) *F Strand = Plus / Minus *F Query: 713 gtaagcggattaaggtttgagacacaactacgtacgttaaatcaattcccggacacgctg 772 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 98575 gtaagcggattaaggtttgagacacaactacgtacgttaaatcaattcccggacacgctg 98516 *F Query: 773 cttggggatccagctcggagattacggtactttgacccgcttagaaatgaatattttttt 832 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 98515 cttggggatccagctcggagattacggtactttgacccgcttagaaatgaatattttttt 98456 *F Query: 833 gaccgtagtcgaccgagcttcgatgcgattttatactattatcagagtggt 883 *F ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 98455 gaccgtagtcgaccgagcttcgatgcgattttatactattatcagagtggt 98405 *F Score = 180 bits (91), Expect = 4e-43 *F Identities = 98/99 (98%), Gaps = 1/99 (1%) *F Strand = Plus / Minus *F Query: 881 ggtggccgactacggagaccggtcaatgtccctttagacg-tatttagtgaagaaataaa 939 *F |||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 98193 ggtggccgactacggagaccggtcaatgtccctttagacggtatttagtgaagaaataaa 98134 *F \* Insertion in genome *F Query: 940 attttatgaattaggtgatcaagcaattaataaattcag 978 *F ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 98133 attttatgaattaggtgatcaagcaattaataaattcag 98095 *F Score = 400 bits (202), Expect = e-109 *F Identities = 202/202 (100%) *F Strand = Plus / Minus *F Query: 976 cagagaggatgaaggctttattaaagaggaagaaagaccattaccggataatgagaaaca 1035 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 97031 cagagaggatgaaggctttattaaagaggaagaaagaccattaccggataatgagaaaca 96972 *F Query: 1036 gagaaaagtctggctgctcttcgagtatccagaaagttcgcaagccgccagagttgtagc 1095 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96971 gagaaaagtctggctgctcttcgagtatccagaaagttcgcaagccgccagagttgtagc 96912 *F Query: 1096 cataattagtgtatttgttatattgctatcaattgttatattttgtctagaaacattacc 1155 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96911 cataattagtgtatttgttatattgctatcaattgttatattttgtctagaaacattacc 96852 *F Query: 1156 cgaatttaagcattacaaggtg 1177 *F |||||||||||||||||||||| *F Sbjct: 96851 cgaatttaagcattacaaggtg 96830 *F Score = 250 bits (126), Expect = 5e-64 *F Identities = 126/126 (100%) *F Strand = Plus / Minus *F Query: 1173 aggtgttcaatacaacaacaaatggcacaaaaatcgaggaagacgaggtgcctgacatca 1232 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96733 aggtgttcaatacaacaacaaatggcacaaaaatcgaggaagacgaggtgcctgacatca 96674 *F Query: 1233 cagatcctttcttccttatagaaacgttatgtattatttggtttacatttgaactaactg 1292 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96673 cagatcctttcttccttatagaaacgttatgtattatttggtttacatttgaactaactg 96614 *F Query: 1293 tcaggt 1298 *F |||||| *F Sbjct: 96613 tcaggt 96608 *F Score = 315 bits (159), Expect = 9e-84 *F Identities = 159/159 (100%) *F Strand = Plus / Minus *F Query: 1293 tcaggttcctcgcatgtccgaacaaattaaatttctgcagggatgtcatgaatgttatcg 1352 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96186 tcaggttcctcgcatgtccgaacaaattaaatttctgcagggatgtcatgaatgttatcg 96127 *F Query: 1353 acataatcgccatcattccgtactttataacactagcgactgtcgttgccgaagaggagg 1412 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96126 acataatcgccatcattccgtactttataacactagcgactgtcgttgccgaagaggagg 96067 *F Query: 1413 atacgttaaatcttccaaaagcgccagtcagtccacagg 1451 *F ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 96066 atacgttaaatcttccaaaagcgccagtcagtccacagg 96028 *F Score = 347 bits (175), Expect = 3e-93 *F Identities = 175/175 (100%) *F Strand = Plus / Minus *F Query: 1449 aggacaagtcatcgaatcaggctatgtccttggcaatattacgagtgatacgattagttc 1508 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 95510 aggacaagtcatcgaatcaggctatgtccttggcaatattacgagtgatacgattagttc 95451 *F Query: 1509 gagtatttcgaatatttaagttatctaggcattcgaagggtttacaaatattaggacgaa 1568 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 95450 gagtatttcgaatatttaagttatctaggcattcgaagggtttacaaatattaggacgaa 95391 *F Query: 1569 ctctgaaagcctcaatgcgggaattaggtttacttatatttttcttatttatagg 1623 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 95390 ctctgaaagcctcaatgcgggaattaggtttacttatatttttcttatttatagg 95336 *F Score = 266 bits (134), Expect = 8e-69 *F Identities = 134/134 (100%) *F Strand = Plus / Minus *F Query: 1620 taggcgtcgtactcttctcatcggcggtttattttgcggaagctggaagcgaaaattcct 1679 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 90767 taggcgtcgtactcttctcatcggcggtttattttgcggaagctggaagcgaaaattcct 90708 *F Query: 1680 tcttcaagtccatacccgatgcattttggtgggcggtcgttaccatgaccaccgttggat 1739 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 90707 tcttcaagtccatacccgatgcattttggtgggcggtcgttaccatgaccaccgttggat 90648 *F Query: 1740 atggtgacatgacg 1753 *F |||||||||||||| *F Sbjct: 90647 atggtgacatgacg 90634 *F Score = 359 bits (181), Expect = 7e-97 *F Identities = 190/193 (98%) *F Strand = Plus / Minus *F Query: 1753 gcccgtcggcttctggggcaaaattgtcggctctttgtgcgtggtcgctggtgtgctgac 1812 *F ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||| *F Sbjct: 76205 gcccgtcggcttctggggcaaaattgtcggctctttgtgcgtgatcgctggtgtgctgac 76146 *F Query: 1813 aatcgcactgccggtaccggttatcgtcagtaatttcaattacttctatcaccgcgaagc 1872 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | *F Sbjct: 76145 aatcgcactgccggtaccggttatcgtcagtaatttcaattacttctatcaccgcgaaac 76086 *F Query: 1873 ggatcgggaggagatgcagagccaaaatttcaaccacgttacaagttgttcatatttacc 1932 *F ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 76085 ggatcaggaggagatgcagagccaaaatttcaaccacgttacaagttgttcatatttacc 76026 *F Query: 1933 tggtgcactaggt 1945 *F ||||||||||||| *F Sbjct: 76025 tggtgcactaggt 76013 *F Score = 642 bits (324), Expect = 0.0 *F Identities = 324/324 (100%) *F Strand = Plus / Minus *F Query: 1941 taggtcaacatttgaagaaatcctcactctccgaatcgtcgtcggacataatggatttgg 2000 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75869 taggtcaacatttgaagaaatcctcactctccgaatcgtcgtcggacataatggatttgg 75810 *F Query: 2001 atgatggcattgatgcaaccacgccaggtctgactgatcacacgggccgccacatggtgc 2060 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75809 atgatggcattgatgcaaccacgccaggtctgactgatcacacgggccgccacatggtgc 75750 *F Query: 2061 cgtttctcaggacacagcagtcattcgagaagcagcagctccagcttcagctgcagctgc 2120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75749 cgtttctcaggacacagcagtcattcgagaagcagcagctccagcttcagctgcagctgc 75690 *F Query: 2121 agcagcagtcgcagtcgccgcacggccaacagatgacgcagcagcagcagctgggccaga 2180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75689 agcagcagtcgcagtcgccgcacggccaacagatgacgcagcagcagcagctgggccaga 75630 *F Query: 2181 acggcctaaggagcacaaatagtttacagttaaggcataataacgcgatggccgtcagta 2240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75629 acggcctaaggagcacaaatagtttacagttaaggcataataacgcgatggccgtcagta 75570 *F Query: 2241 ttgagaccgacgtctgactactag 2264 *F |||||||||||||||||||||||| *F Sbjct: 75569 ttgagaccgacgtctgactactag 75546 *F Score = 1306 bits (659), Expect = 0.0 *F Identities = 659/659 (100%) *F Strand = Plus / Minus *F Query: 2263 agtcaaacaaatggaaaatggacgaaatttgcgcagtgaaatgctacgttggatgccaga 2322 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75134 agtcaaacaaatggaaaatggacgaaatttgcgcagtgaaatgctacgttggatgccaga 75075 *F Query: 2323 aacgtcatcaaaagcagtctaatttagaattttattaataaatacaattaaaatataatt 2382 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75074 aacgtcatcaaaagcagtctaatttagaattttattaataaatacaattaaaatataatt 75015 *F Query: 2383 ataataattagtaagcaacgtagttgtaaattaaacagcaaatgtacacagacacaacac 2442 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 75014 ataataattagtaagcaacgtagttgtaaattaaacagcaaatgtacacagacacaacac 74955 *F Query: 2443 acacacagacacagtgccagttcactcagcttgaattagagtatttgtagacaccaaaaa 2502 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74954 acacacagacacagtgccagttcactcagcttgaattagagtatttgtagacaccaaaaa 74895 *F Query: 2503 gagtcaaatatggactggccttctatagggatttccttgtttctcctttcattttccttc 2562 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74894 gagtcaaatatggactggccttctatagggatttccttgtttctcctttcattttccttc 74835 *F Query: 2563 tggtaatctacacaccgaaaacacttacacacacacgtccacacacactcaaagtaaaaa 2622 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74834 tggtaatctacacaccgaaaacacttacacacacacgtccacacacactcaaagtaaaaa 74775 *F Query: 2623 ctctacttgatacctatgttcaaatttagcaattaacaactaacaatcgttaacaacaac 2682 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74774 ctctacttgatacctatgttcaaatttagcaattaacaactaacaatcgttaacaacaac 74715 *F Query: 2683 aaaacaaaacatataaaaccaaaaaacgagagaaaaaaaaaaacaaacaaaaccaaaatc 2742 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74714 aaaacaaaacatataaaaccaaaaaacgagagaaaaaaaaaaacaaacaaaaccaaaatc 74655 *F Query: 2743 taattatcttagtagactaatctaattggagtttcttcctttctttagaagctagcaaaa 2802 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74654 taattatcttagtagactaatctaattggagtttcttcctttctttagaagctagcaaaa 74595 *F Query: 2803 caaaaacaaagaacaacaacaaccagacaaaaacaaacatacaatatctgctaattttat 2862 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74594 caaaaacaaagaacaacaacaaccagacaaaaacaaacatacaatatctgctaattttat 74535 *F Query: 2863 tttcatctttaaattatgctctattattaaatattagtcagaatattagtaaaacaaac 2921 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 74534 tttcatctttaaattatgctctattattaaatattagtcagaatattagtaaaacaaac 74476 *F Browser: Mozilla/4.73 en (Win98; I) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135197 *a Rawlings *b N.D. *t 2001.2.12 *T personal communication to FlyBase *u FlyBase error report on Mon Feb 12 15:48:19 2001. *F From neil.rawlings@bbsrc.ac.uk Mon Feb 12 15:59:10 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Feb 2001 15:59:10 \+0000 *F From: 'neil rawlings (BI)' *F To: 'Gillian Millburn' *F Cc: flybase-help@morgan.harvard.edu *F Subject: Another new peptidase gene in Drosophila *F Date: Mon, 12 Feb 2001 15:48:19 \-0000 *F MIME-Version: 1.0 *F X-Mailer: Internet Mail Service (5.5.2650.21) *F Content-Type: multipart/mixed; boundary='----_=_NextPart_000_01C0950B.397E3448' *F Content-Length: 7745 *F Dear Dr Milburn, *F I believe I have discovered a second previously unannotated gene in the *F Drosophila genome. Sequence similarity was detected by a TBlastN search of *F the NR database at NCBI using the sequence of the Thermoplasma proteasome *F beta component (peptidase family T1) as a probe. A match was found with *F GenBank entry AE003657, section 50 of 63 of the genomic scaffold *F 142000013386055 of Drosophila. The Drosophila homologue occupies bases *F 100613-101452 and is a continuous open reading frame from initiation *F methionine to stop codon: there are no introns. I attach a file *F (AE003657.Ret) showing a TFastX-derived alignment between this Drosophila *F sequence and CG12323 protein (AAF58748 \- another Drosophila proteasome beta *F subunit), and a file containing the complete amino acid sequence of the *F newly identified gene (AE003657.AA). *F In GenBank entry AE003657 the region 100613-101452 has not been defined as *F containing a gene/ *F ============================================================================ *F == *F Dr Neil D. Rawlings *F MRC Molecular Enzymology Laboratory *F Babraham Institute *F Babraham Hall *F Babraham *F Cambridgeshire *F CB2 4AT *F UK *F Tel: \+1223 496649 *F Fax: \+1223 496023 *F mailto:neil.rawlings@bbsrc.ac.uk *F ============================================================================ *F == *F Please visit the MEROPS database, the URL is: *F http://WWW.MEROPS.CO.UK/merops/Merops.htm *F ============================================================================ *F AE003657.Ret: *F TFASTXY compares a protein to a translated DNA data bank *F version 3.2t06 July 29, 1999 *F Please cite: *F Pearson et al, Genomics (1997) 46:24-36 *F aaseq\AAF58748.aa, 282 aa *F vs AE003657.dna library *F 256010 residues in 1 sequences *F TFASTX (3.26 July 1999) function optimized, BL50 matrix (15:-5) ktup: 2 *F join: 36, opt: 24, gap-pen: \-15/ \-2 shift: \-20, width: 16 reg.-scaled *F The best scores are: initn init1 opt *F AE003657 \- DNA sequence (99702) f 949 886 1006 *F AE003657 \- DNA sequence (99702) r 118 49 57 *F AE003657 \- DNA sequence (58298) r 94 44 57 *F AE003657 \- DNA sequence (99702) r 115 52 52 *F AE003657 \- DNA sequence (58298) f 70 47 51 *F AE003657 \- DNA sequence (99702) f 89 45 48 *F >>AE003657 \- DNA sequence (99702 aa) *F Frame: f initn: 949 init1: 886 opt: 1006 *F Smith-Waterman score: 1006; 51.786% identity in 280 aa overlap *F (1-279:100613-101443) *F 10 20 30 40 50 *F gi|730 MALAEICKISNAPYMRPNAW-SSADVEEEQKGLMCNLANPYTLAAPPFENPLHNLNQIQA *F ::: :: ... :.:. .. .: .. :: . :. :: .. :::.::: .......: *F AE0036 MALESICGMDKLPFMKSFGYRTSKQTIEEIRVASSNMDNPLAIMAPPYENPRESVKKLNA *F 100640 100670 100700 100730 100760 100790 *F 60 70 80 90 100 110 *F gi|730 NGDKTGVKINFDHGTTTLGFKFKGGVLLAVDSRATGGSYIGSQSMKKIVEINQFMLGTLA *F .. :.:.:::::::.:: ..::..: ::::::.:. :::::..:.:..::...:: : *F AE0036 LSE---VQIDFDHGTTTVGFVYQGGIILCVDSRATSGKLIGSQSIHKVVQVNQYIMGTTA *F 100820 100850 100880 100910 100940 *F 120 130 140 150 160 170 *F gi|730 GGAADCVYWDRVLSKECRLHELRNKERISVAAASKIMANIAHEYKGMGLSMGMMLAGYDK *F ::::::.::::.:..:::::::: :::. : .:.: ..:.: ::::::: :::::::.. *F AE0036 GGAADCTYWDRALTRECRLHELRYKERLPVQSAAKYISNVAAEYKGMGLCMGMMLAGWSP *F 100970 101000 101030 101060 101090 101120 *F 180 190 200 210 220 230 *F gi|730 RGPGLYYVDSEGSRTPGNLFSVGSGSLYAYGVLDSGYHWDLEDKEAQELGRRAIYHATFR *F .::.: ::::.: : :.::.::::. : :.::: :. :: :.:: .:. :.::::. *F AE0036 EGPSLVYVDSNGLRIHGKLFAVGSGAPNALGILDSDYRLDLSDNEAYDLAFLAVYHATMT *F 101150 101180 101210 101240 101270 101300 *F 240 250 260 270 *F gi|730 DAYSGGIIRVYHIKEDGWVNISNTDCMELHYMYQEQLKQQ *F : .:::..:.::. . .: :..: ::.::: .:. .:: *F AE0036 DIFSGGVVRLYHMDQGNWRNVANKDCQELHEQYSGVGNQQ *F 101330 101360 101390 101420 *F >>AE003657 \- DNA sequence (99702 aa) *F Frame: r initn: 118 init1: 49 opt: 57 *F Smith-Waterman score: 58; 33.333% identity in 27 aa overlap (68-94:63554-63474) *F 70 80 90 *F gi|730 INFDHGTTTLGFKFKGGVLLAVDSRAT *F .....:: .. . :::..: .::. : *F AE0036 VSMQNGTMYISENCKGGTFLPLDSHPT *F 63540 63510 63480 *F >>AE003657 \- DNA sequence (58298 aa) *F Frame: r initn: 94 init1: 44 opt: 57 *F Smith-Waterman score: 58; 29.268% identity in 41 aa overlap *F (14-51:252897-252775) *F 20 30 40 50 *F gi|730 YMRPNAWSSADVEE---EQKGLMCNLANPYTLAAPPFENPL *F : : ::. ..:. .. .: ..: .:: : ..: : *F AE0036 YGRVRAWQPLNIEQIRGTWNNKICPISNXGNLAFPXIRNXL *F 252880 252850 252820 252790 *F >>AE003657 \- DNA sequence (99702 aa) *F Frame: r initn: 115 init1: 52 opt: 52 *F Smith-Waterman score: 61; 32.653% identity in 49 aa overlap *F (49-96:142982-142839) *F 50 60 70 80 90 *F gi|730 NPLH-NLNQIQANGDKTGVKINFDHGTTTLGFKFKGGVLLAVDSRATGG *F : :: : :: ...: .:. . : .:.::.. . :.: .: : *F AE0036 NGLHXNXNQTKTQG-XNGITTTVTHQATALGYSNHTEDLIASXLKARRG *F 142980 142950 142920 142890 142860 *F >>AE003657 \- DNA sequence (58298 aa) *F Frame: f initn: 70 init1: 47 opt: 51 *F Smith-Waterman score: 69; 20.548% identity in 73 aa overlap *F (6-74:198602-198820) *F 10 20 30 40 50 60 *F gi|730 ICKISNAPYMRPNAWSSADVE----EEQKGLMCNLANPYTLAAPPFENPLHNLNQIQANG *F .:.:. .: .:. ::. .. . :.: :.. ..: : . . . . : *F AE0036 MCSINXIAKIRTSAFVSAEYVXMFIDQPSHLLCALSTIFALKPQKFCXIFIKNTWLGNCG *F 198620 198650 198680 198710 198740 198770 *F 70 *F gi|730 DKTGVKINFDHGT *F . .:.:... :. *F AE0036 SCVGIKMSLRSGS *F 198800 *F >>AE003657 \- DNA sequence (99702 aa) *F Frame: f initn: 89 init1: 45 opt: 48 *F Smith-Waterman score: 60; 26.087% identity in 46 aa overlap (23-68:80498-80635) *F 30 40 50 60 *F gi|730 ADVEEEQKGLMCNLANPYTLAAPPFENPLHNLNQIQANGDKTGVKI *F ::: . . : . .: .: :..:: .. .. :.:. .. :. *F AE0036 ADVLQIDPGPSSIFRSPEVLFQKRFQSPLDDFXHVLAGGSGNSHKL *F 80510 80540 80570 80600 80630 *F 282 residues in 1 query sequences *F 256010 residues in 1 library sequences *F Scomplib version 3.2t06 July 29, 1999 *F start: Fri Feb 09 14:55:45 2001 done: Fri Feb 09 14:56:32 2001 *F Scan time: 0.000 Display time: 47.000 *F Function used was TFASTXY *F ============================================================================ *F AE003657.AA: *F >AE003657 \- proteasome beta subunit, Drosophila melanogaster *F MALESICGMDKLPFMKSFGYRTSKQTIEEIRVASSNMDNPLAIMAPPYENPRESVKKLNA *F LSEVQIDFDHGTTTVGFVYQGGIILCVDSRATSGKLIGSQSIHKVVQVNQYIMGTTA *F GGAADCTYWDRALTRECRLHELRYKERLPVQSAAKYISNVAAEYKGMGLCMGMMLAGWSP *F EGPSLVYVDSNGLRIHGKLFAVGSGAPNALGILDSDYRLDLSDNEAYDLAFLAVYHATMT *F DIFSGGVVRLYHMDQGNWRNVANKDCQELHEQYSGVGNQQ *F ============================================================================ # *U FBrf0135198 *a Levis *b R. *t 2001.1.26 *T personal communication to FlyBase *u FlyBase error report for CG17600 and CG17602 on Thu Jan 25 19:27:34 2001. *F From levis@ciwemb.edu Fri Jan 26 00:26:38 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 26 Jan 2001 00:26:38 \+0000 *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Thu, 25 Jan 2001 19:27:34 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: cDNA LD42024 suggests CG17600 and CG17602 are part of same gene *F The cDNA clone LD42024 is assigned to the S6kII (CG17596) gene in the *F BDGP database. However, the BLAST of LD42024.5prime hits CG17602, *F which is upstream of S6kII and LD42024.3prime hits CG17600, which is *F downstream of S6kII. If this is a bona fide cDNA, it would suggest *F that CG17602 and CG17600 are part of the same transcript and *F therefore likely part of the same gene. I haven't analyzed any of *F the other ESTs hit by LD42024, to see if this is consistent with other *F cDNAs. *F Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210 *F phone: (410) 554-1238 *F fax: (410) 467-1147 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0135199 *a Levis *b R. *t 2001.2.1 *T personal communication to FlyBase *u FlyBase error report on Thu Feb 1 22:46:48 2001. *F From levis@ciwemb.edu Fri Feb 02 03:45:57 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 2 Feb 2001 03:45:57 \+0000 *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Thu, 1 Feb 2001 22:46:48 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: CG3013 and CG17501 are not genes, they're part of a *F transposon-related repetitive element family *F Content-Type: multipart/mixed; *F boundary='============_-1231036887==_============' *F Content-Length: 27220 *F I became suspicious that CG3013 and CG17501 may not really be genes *F when I saw that both share homologous domains with C. elegans Tc1 *F transposase. When I BLASTed each against the Drosophila genome, I *F got strong hits to numerous scaffold segments (see attached text file *F of results with CG3013). The segments share >90% identity over a *F region of > 500 nts. I suspect, because of the homology to Tc1 *F transposase, that this repetitive family is or was a family of short *F inverted repeat type DNA transposons. *F Furthermore, the N-terminus of the CG8415 protein, is also related to *F this transposon-like repetitive sequence family. Here is a BLASTn *F alignment of a portion of AE003788, which contains CG3013, with *F AE003815, which contains CG8415. *F Query: 121992 *F tcaagcaacatgatccgcaaagcactgctgtttgtcgagaagaacgaaacacggcgaaga 122051 *F |||| ||| ||||||||||||||||||||||||||||| *F ||||||||||||||||||||| *F Sbjct: 92707 *F tcaaacaaaatgatccgcaaagcactgctgtttgtcgaaaagaacgaaacacggcgaaga 92648 *F Query: 122052 *F aagccctctatgtccaacgtggagataaagcgcttggttcggcaaggcaagaagga 122107 *F |||||||| ||||||||||||||||| | *F |||||||||||||||| |||||||||| *F Sbjct: 92647 *F aagccctcaatgtccaacgtggagatcaggcgcttggttcggcaaagcaagaagga 92592 *F CG8415 gene product 1 M S N V E I R R L V R *F Q S K K D *F Score = 166 bits (86), Expect = 4e-36 *F Identities = 102/110 (92%) *F Strand = Plus / Minus *F Query: 121687 *F tctctcaatgtaacggttctttttgtttttgggcacttgctgcaaaagtgcgcgaaatga 121746 *F |||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| | *F Sbjct: 92850 *F tctctcaatgtaacggttctttttgtttttggtcacttgctgcaaaagtgcgcgaaatta 92791 *F Query: 121747 ggcgttaaccaaaatagcactgaccacgtatttgctgaataaaactaata 121796 *F |||| |||| ||||||| ||||| ||||||||||||||||||| ||||| *F Sbjct: 92790 ggcggtaacaaaaatagtactgattacgtatttgctgaataaaattaata 92741 *F Score = 164 bits (85), Expect = 1e-35 *F Identities = 93/97 (95%) *F Strand = Plus / Minus *F ImageImageImageImage *F ImageImageImage *F Query: 122318 *F agatgagaccaaaattgtgttgtttggtgggaaaggctcttggtcttatgttcgtcgtcc 122377 *F |||||||| *F ||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 92588 *F agatgagagcaaaattgtgttgtttggtgggaaaggctcttggtcttatgttcggcgtcc 92529 *F CG8415 gene product 18 D E S K I V L F *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Query: 122378 accacgaactgaatttaatcctcgcttcacctttaag 122414 *F |||||||||||||| ||||| |||||||||||||||| *F Sbjct: 92528 accacgaactgaatataatcatcgcttcacctttaag 92492 *F CG8415 gene product 92528 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Score = 164 bits (85), Expect = 1e-35 *F Identities = 107/118 (90%) *F Strand = Plus / Minus *F Query: 122459 *F aaatcattgtagtatttaagttaagtgagtaaaatgatgaaaaagtggcagaaaatagag 122518 *F |||||| ||||||||||||||| ||| || || *F |||||||||||||| |||||||| || *F Sbjct: 92363 *F aaatcactgtagtatttaagtttagtcagcaatatgatgaaaaagtgttagaaaatacag 92304 *F CG8415 gene product 92363 *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Query: 122519 *F aaacgctgggacatacacgaaatgtgcttatggtgctattattgttaccgcaactgta 122576 *F ||||| |||| || *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 92303 *F aaacggtgggccaaacacgaaatgtgcttatggtgctattattgttaccgcaactgta 92246 *F CG8415 gene product 92303 *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Score = 110 bits (57), Expect = 2e-19 *F Identities = 101/118 (85%), Gaps = 8/118 (6%) *F Strand = Plus / Minus *F Query: 121553 *F tacagctgcggttaaaataatagcactactgcaggtgaaaagttcatttcataatcataa 121612 *F ||||| |||||||||||||||| ||||||||||| || |||||| ||||| ||| | || *F Sbjct: 92987 *F tacagttgcggttaaaataataacactactgcagttgcaaagttgatttcctaa--aaaa 92930 *F Query: 121613 aattattaaatgtttatattttttaaagtcagattgcatgaataataagtaccatatg 121670 *F ||||||||||| ||||| | ||||||||||||||||||||||||||||||||| *F Sbjct: 92929 aattattaaatatttattt------aagtcagattgcatgaataataagtaccatatg 92878 *F CG8415 has been characterized as a ribosomal protein gene. I haven't *F looked to see what portion of CG8415 is homologous to ribosomal *F proteins. It seems likely to me that the N-terminal coding exon of *F CG8415 isn't really part of the ribosomal protein, but instead is a *F remnant of a transposable element. I think the real N-terminus of *F the ribosomal protein is probably located downstream of the *F repetitive sequence. *F ================================================================================ *F Query= *F (585 letters) *F Database: D. melanogaster genomic nucleotide sequences *F 1170 sequences; 122,655,632 total letters *F If you have any problems or questions with the results of this search *F please refer to the BLAST FAQs *F Taxonomy reports *F Distribution of 91 Blast Hits on the Query Sequence *F Image *F \------------------------------------------------------------------------ *F Score E *F Sequences producing significant alignments: *F (bits) Value *F gb|AE003788.2|AE003788 Drosophila melanogaster genomic scaf... 1160 0.0 *F gb|AE003040.1|AE003040 Drosophila melanogaster genomic scaf... 890 0.0 *F gb|AE003066.1|AE003066 Drosophila melanogaster genomic scaf... 874 0.0 *F gb|AE002770.1|AE002770 Drosophila melanogaster genomic scaf... 844 0.0 *F gb|AE003786.2|AE003786 Drosophila melanogaster genomic scaf... 724 0.0 *F gb|AE003512.2|AE003512 Drosophila melanogaster genomic scaf... 722 0.0 *F gb|AE002582.2|AE002582 Drosophila melanogaster genomic scaf... 640 0.0 *F gb|AE003845.2|AE003845 Drosophila melanogaster genomic scaf... 525 e-148 *F gb|AE003846.2|AE003846 Drosophila melanogaster genomic scaf... 496 e-139 *F gb|AE003603.2|AE003603 Drosophila melanogaster genomic scaf... 486 e-136 *F gb|AE003212.1|AE003212 Drosophila melanogaster genomic scaf... 482 e-135 *F gb|AE003843.2|AE003843 Drosophila melanogaster genomic scaf... 365 e-100 *F gb|AE003395.2|AE003395 Drosophila melanogaster genomic scaf... 361 1e-98 *F gb|AE003389.2|AE003389 Drosophila melanogaster genomic scaf... 351 1e-95 *F gb|AE003090.1|AE003090 Drosophila melanogaster genomic scaf... 351 1e-95 *F gb|AE003657.2|AE003657 Drosophila melanogaster genomic scaf... 270 3e-71 *F gb|AE003605.2|AE003605 Drosophila melanogaster genomic scaf... 266 5e-70 *F gb|AE003019.2|AE003019 Drosophila melanogaster genomic scaf... 194 2e-48 *F gb|AE003815.2|AE003815 Drosophila melanogaster genomic scaf... 174 2e-42 *F gb|AE003594.1|AE003594 Drosophila melanogaster genomic scaf... 170 2e-41 *F gb|AE003844.2|AE003844 Drosophila melanogaster genomic scaf... 135 1e-30 *F gb|AE002772.2|AE002772 Drosophila melanogaster genomic scaf... 109 7e-23 *F gb|AE002837.1|AE002837 Drosophila melanogaster genomic scaf... 94 4e-18 *F gb|AE003787.2|AE003787 Drosophila melanogaster genomic scaf... 88 3e-16 *F gb|AE002942.2|AE002942 Drosophila melanogaster genomic scaf... 86 1e-15 *F gb|AE003574.3|AE003574 Drosophila melanogaster genomic scaf... 84 4e-15 *F gb|AE003147.2|AE003147 Drosophila melanogaster genomic scaf... 54 4e-06 *F gb|AE003573.2|AE003573 Drosophila melanogaster genomic scaf... 50 6e-05 *F Alignments *F tmpseq_0 1 atgggtcgcggaaagcattgtaccgtcgaaaaaagaaatttgattaaaaacatgatctct 60 *F AE003788 121890 *F ............................................................ 121949 *F AE003040 40718 *F .....................c........-......................... 40772 *F AE003066 3668 *F ...................................................t........ 3609 *F AE002770 13094 *F ............................................................ 13035 *F AE003786 26889 *F ..........a................................................. 26830 *F AE003512 297556 *F ........................................................t... 297615 *F AE003846 36955 *F .......................................t..........c..--... 37010 *F AE003846 154469 *F .......a...........................................g........ 154410 *F AE003846 174621 *F .......a...........................................g........ 174562 *F AE003846 213790 ...........c......... *F 213770 *F AE003603 22141 *F ...........................t................................ 22200 *F AE003212 788 *F ..............t............-......a.......g................. 846 *F AE003843 211063 *F ...........................t..................t...... 211011 *F AE003843 312639 ........t....................................... *F 312592 *F AE003395 19920 ............................................................ *F 19861 *F AE003389 8967 *F ............................................................ 9026 *F AE003090 22654 *F ..........t... 22641 *F AE003090 22674 ................. *F 22658 *F AE003657 222682 *F ...........c.......t........................................ 222741 *F AE003019 648 *F ......t......t.................. 617 *F AE003594 166750 *F .........at................................................. 166691 *F AE003844 146102 *F ..................................................t......... 146043 *F AE003451 169882 *F .................. 169865 *F AE003796 68203 .................. *F 68220 *F AE003756 188259 .................. *F 188242 *F AE003193 10738 ................. *F 10722 *F AE002656 179535 ................. *F 179519 *F AE003506 80304 .......g............. *F 80284 *F tmpseq_0 61 *F gaaggtaaaaa-atacgctgaaattggccgcattgtcggttgttcaagcaacatgatccg 119 *F AE003788 121950 *F ...........-................................................ 122008 *F AE003040 40773 *F ...........-.....a.........t.......................a........ 40831 *F AE003066 3608 *F ...........-...............t....................t..a........ 3550 *F AE002770 13034 *F ...........-...............t......t.......g........a........ 12976 *F AE003786 26829 *F ...t......c-c..............t..a........g.......a...a.......a 26771 *F AE003512 297616 *F ...c......c-c..............t...................a...a........ 297674 *F AE003846 37011 *F ...........-...............t................t..a...a........ 37069 *F AE003846 154409 *F ..........c-c..............t.......................a........ 154351 *F AE003846 174561 *F ..........c-c..............t...................a...a........ 174503 *F AE003846 250813 *F ..............a...a.....a.. 250839 *F AE003603 22201 *F ..........c-c..........g...t..a................a...a.......a 22259 *F AE003212 847 *F ....t......-..c............t.............a.........a........ 905 *F AE003843 211006 *F ........t..a...t............a...a........ 210966 *F AE003843 312593 *F .........c-c..............t........t..........a...a..a..... 312536 *F AE003843 211010 ..... *F 211006 *F AE003395 19860 *F ..........c-c...t..........t..............c.g..a...a........ 19802 *F AE003389 9027 *F ...........cc...t..........t..............c.g..a...a........ 9086 *F AE003090 22640 *F a..........-...............t...................a...a..a..... 22582 *F AE003657 222742 *F a..........-...............tg.......g............t.a........ 222800 *F AE003019 616 *F .........tc-c..............t.......................a....c... 558 *F AE003815 92707 *F ....a...a........ 92691 *F AE003594 166690 ..........c-c..............a................... *F 166645 *F AE003844 146042 ...........-. *F 146031 *F tmpseq_0 120 *F caaagcac-t-gctgtttgtcgagaagaacgaaacacggcgaagaaagccctctatgtcc 177 *F AE003788 122009 *F ........-.-................................................. 122066 *F AE003040 40832 *F ........-.-a...........a.....a.......................a...... 40889 *F AE003066 3549 *F ........-.-............a.............................a...... 3492 *F AE002770 12975 *F ........-.-...........ga.............................a..a... 12918 *F AE003786 26770 *F ...------.-............a...g..........tg.............a...... 26718 *F AE003512 297675 *F ........-.-............a.....g...............g.......a...... 297732 *F AE002582 10683 *F ..........a.............................a...... 10637 *F AE003846 37070 *F ........-.-............a.............................a...... 37127 *F AE003846 154350 *F .......g-.-............a.............................a...... 154293 *F AE003846 174502 *F ....t...-.-............a...........g......g.......t..a...... 174445 *F AE003846 250840 ........-.-............a.............................a...... *F 250897 *F AE003603 22260 *F ...t....-.-............a...g..........tga............a...... 22317 *F AE003212 906 *F ........-.-.a......g...a.....................t.......a...... 963 *F AE003843 210965 *F ........-.-............a.............................a...... 210908 *F AE003843 312535 *F ...c....-.-............a...g..........tg.............a...... 312478 *F AE003395 19801 ........-.-............a............ *F 19768 *F AE003389 9087 ........t.-............a............ *F 9121 *F AE003090 22581 *F ........-.-............a...g..........tg.............a...... 22524 *F AE003657 222801 *F ........-.-............a.............................a..... 222857 *F AE003019 557 *F ...c....-aa......aa....a..t.............a......t.....a...... 499 *F AE003815 92690 *F ........-.-............a.............................a...... 92633 *F AE003594 166638 *F ............a...... 166620 *F AE002772 1062 *F ......a.....g.....c..tg.............a...... 1104 *F tmpseq_0 178 *F aa-cgtggagataaagcg-cttggttcggcaaggcaagaaggagccttttaagccggcga 235 *F AE003788 122067 *F ..-...............-......................................... 122124 *F AE003040 40890 *F ..-.........c.....-......................................... 40947 *F AE003066 3491 *F ..-.........c.....-......................................... 3434 *F AE002770 12917 *F ..-.........c.....-......................................t.. 12860 *F AE003786 26717 *F ..-.........c.....-.............a........................... 26660 *F AE003512 297733 *F ..-.........c.....-.......t.....a...........t............... 297790 *F AE002582 10636 *F ..-.........c.....-......................................... 10579 *F AE003845 87346 *F ................................t.. 87312 *F AE003846 37128 *F ..-.........c.....-...........................g...........a. 37185 *F AE003846 154292 .. *F 154291 *F AE003846 174444 ..-.t.... *F 174437 *F AE003846 250898 ..-......---c.....-.......t.....a........ *F 250933 *F AE003846 174420 .....-.............a....... *F 174395 *F AE003846 174393 *F ....t.. 174387 *F AE003603 22318 *F ..-.........c.....-.............a........................... 22375 *F AE003212 964 *F ..-.........cg....-..............a.......................... 1021 *F AE003843 210907 *F ..-...............-.......t.....a................a.......... 210850 *F AE003843 312477 ..-.........c.....-............. *F 312448 *F AE003090 22523 *F ..-....a....c.....-.............a.............g..a.......t.. 22466 *F AE003657 222857 *F ..-.......a......................a.......... 222899 *F AE003019 498 ..-.........c.....-........... *F 471 *F AE003815 92632 ..-.........c.g...-.............a.......... *F 92592 *F AE003594 166619 *F ..-...t.....c....c-.........a...----------.................. 166572 *F AE002772 1105 *F ..c.....c...c.....c.............a........................... 1164 *F tmpseq_0 236 *F cggaactggagaaggagcttcagatagctgaaagcgtggaaactgttcgcaaacgcttag 295 *F AE003788 122125 *F ............................................................ 122184 *F AE003040 40948 *F ........a................................................... 41007 *F AE003066 3433 *F .......ca................................................... 3374 *F AE002770 12859 *F ........ag.................................................. 12800 *F AE003786 26659 *F ........a.....................c............a...............a 26600 *F AE003512 297791 *F ........a.....................c............a...............a 297850 *F AE002582 10578 *F .......ta...........a....................................... 10519 *F AE003845 87311 *F ........a....c.................................g............ 87252 *F AE003846 37186 *F ........t......................................t......t....a 37245 *F AE003846 174386 ........a...t............. *F 174361 *F AE003603 22376 *F ........a.....................c............a...............a 22435 *F AE003212 1022 ........a.............................a....a................ *F 1081 *F AE003843 210849 *F ........a.a...................c............a................ 210790 *F AE003843 312442 *F ........t.....a 312428 *F AE003395 19763 *F ....................a.............................. 19713 *F AE003389 9126 *F ....................a.............................. 9176 *F AE003090 22458 *F .........t...........a 22437 *F AE003090 22465 ........ *F 22458 *F AE003657 222900 ........a...........................g........ *F 222944 *F AE003594 166571 ........a........... *F 166552 *F AE002772 1165 .... *F 1168 *F tmpseq_0 296 *F gacaaaacaaccttaatgcgtgcagcccaaataaagtcccccttttgactgttaagcatg 355 *F AE003788 122185 *F ............................................................ 122244 *F AE003040 41008 *F ...............................-............................ 41066 *F AE003066 3373 *F ..............................ga........g................... 3314 *F AE002770 12799 *F ..............................ga...............g.......t.... 12740 *F AE003786 26599 *F .........................t..c.ga........g................... 26540 *F AE003512 297851 *F ............................c..a.....a..g...........g....... 297910 *F AE002582 10518 *F ..............................ga........g................... 10459 *F AE003845 87251 *F ..............................ga........g..................a 87192 *F AE003846 37246 ............................ *F 37273 *F AE003846 37276 *F ...........t...... 37293 *F AE003603 22436 *F ........c......t.........t..c.ga........g................... 22495 *F AE003212 1082 ..........t...................gg........g....... *F 1129 *F AE003843 210789 .....................a..... *F 210763 *F AE003843 210204 *F ........g................... 210177 *F AE003843 312427 ......................... *F 312403 *F AE003395 19712 *F ......g.....................c.ga........g................... 19653 *F AE003389 9177 *F ......g.....................c.ga........g................... 9236 *F AE003090 22436 *F ............................c.ga........g................... 22377 *F AE003605 264815 *F .............................ga........g................... 264757 *F AE003732 119015 ................... *F 118997 *F tmpseq_0 356 *F tggcaaagc-gaatcgaatatgccaagattcccaaggactggcctgt-ggagatgtggca 413 *F AE003788 122245 *F .........-.....................................-............ 122302 *F AE003040 41067 *F .........-.....................................-.....a...... 41124 *F AE003066 3313 *F .........-...c............................gt...-.....a...... 3256 *F AE002770 12739 *F .........t.........................c...........-.....a...... 12681 *F AE003786 26539 *F .........-..c................a.a...............-.....a.....g 26482 *F AE003512 297911 *F .........-...................a.a.............a.-.....a....tg 297968 *F AE002582 10458 *F .........-...c............................gt...-.....a...... 10401 *F AE003845 87191 *F .........-....................t................-.....a...... 87134 *F AE003846 37294 .........-..............t.... *F 37321 *F AE003603 22496 ...... *F 22501 *F AE003603 22508 *F ........c.....a-.....a.....g 22534 *F AE003212 1128 *F ...........................t....-.....a.....g 1171 *F AE003843 210176 *F .........-a..........---.....a.a...............-.....a.....g 210122 *F AE003843 312371 *F ...............-.....ac..aag 312345 *F AE003395 19652 *F .........-a.t.t.......a......a.a.......g.......-.....a.....g 19595 *F AE003389 9237 *F .c.......-............a.......t................g.....a...... 9295 *F AE003090 22376 *F .........-...................a.a..........t....-.....a...c.g 22319 *F AE003605 264756 .........-....a................................-.....a.....g *F 264699 *F AE003784 186645 *F . 186645 *F AE003477 267305 ................. *F 267321 *F tmpseq_0 414 *F caacattttgtggtcagatgagaccaaaattgtgttgtttggtgggaaaggctcttggtc 473 *F AE003788 122303 *F ............................................................ 122362 *F AE003040 41125 *F ............................................................ 41184 *F AE003066 3255 *F .......................................c.....a.............. 3196 *F AE002770 12680 *F ....................................a.......a............... 12621 *F AE003786 26481 *F .......................g...............................c.... 26422 *F AE003512 297969 *F .......................t...............................c.... 298028 *F AE002582 10400 *F .............................................a.............. 10341 *F AE003845 87133 *F .................................c...................tc..... 87074 *F AE003846 154294 *F .........................t......a.... 154258 *F AE003846 250937 *F ..........................c.... 250967 *F AE003603 22535 *F ....g..................g...............................c..a. 22594 *F AE003212 1172 *F .......................................................c.... 1231 *F AE003843 210121 *F .......................g.............................t.c.... 210062 *F AE003843 312344 ..................... *F 312324 *F AE003395 19594 *F a........t...........a.g.............c.................c.... 19535 *F AE003389 9296 *F .......................g.............c.................c.... 9355 *F AE003090 22318 *F ...................a...g...............................c.... 22259 *F AE003605 264698 ......................................... *F 264658 *F AE003605 264652 *F ........ 264645 *F AE003815 92588 *F ........g.................................... 92544 *F AE003652 159253 ................. *F 159237 *F AE003784 186644 ................ *F 186629 *F tmpseq_0 474 *F ttatgttcgtcgtccaccacga--------actgaatttaatcctc-g-cttcaccttta 523 *F AE003788 122363 *F ......................--------................-.-........... 122412 *F AE003040 41185 *F ........ag............--------.......a........-.-........... 41234 *F AE003066 3195 *F .........g..........a.--------.......a........-.-........... 3146 *F AE002770 12620 *F .........g............--------.......a.....t..-.-........... 12571 *F AE003786 26421 *F .........g............--------.......a........-.-........... 26372 *F AE003512 298029 *F .........g............cgtccacc...a...a........-.-........... 298086 *F AE002582 10340 *F .........g..........a.--------.......a.......t-.-........... 10291 *F AE003845 87073 *F .........g............--------.......a........-.-........... 87024 *F AE003846 154257 *F .........g.ta.....g...--------.......a........-.g........... 154207 *F AE003846 250968 .a.......g............--------..c..... *F 250997 *F AE003603 22595 *F .........g..........a.--------.......a........-.-........... 22644 *F AE003212 1232 *F .........g............--------..a....a........g.-........... 1282 *F AE003843 210061 *F .........g............--------.......a........-a-........... 210012 *F AE003843 312324 *F .......g..c.........--------.......a.....a..-.-........... 312277 *F AE003395 19534 *F .........g............--------.......a........-.-t.a........ 19485 *F AE003389 9356 ....... *F 9362 *F AE003090 22258 *F .........g............--------.......a........-.g........... 22208 *F AE003605 264644 *F .........g.....t......--------.......a........-.-........... 264595 *F AE003815 92543 *F .........g............--------.......a.....a..-.-........... 92494 *F AE003638 84624 *F ... 84622 *F AE003608 216061 *F ..... 216065 *F AE003510 192026 *F ..... 192022 *F AE003506 201078 *F .... 201081 *F tmpseq_0 524 *F agatctttaagattagatgtgttactaa-tttttttatttcgattgaaaatcattgtagt 582 *F AE003788 122413 *F ............................-............................... 122471 *F AE003040 41235 .. *F 41236 *F AE003040 41795 *F .......c.........-...........t................... 41842 *F AE003066 3145 .. *F 3144 *F AE003066 2508 *F .......c.......---...........................g... 2463 *F AE002770 12570 .. *F 12569 *F AE003786 26371 .. *F 26370 *F AE003786 25734 *F .........-............................... 25695 *F AE003512 298087 .. *F 298088 *F AE003512 298698 *F .......c.........-............................... 298745 *F AE002582 10290 .. *F 10289 *F AE002582 9658 *F .......c.........-............................... 9611 *F AE003845 87023 .. *F 87022 *F AE003845 86386 *F .........-..........................t.... 86347 *F AE003846 154206 .. *F 154205 *F AE003846 37876 *F .......c.........t............................... 37924 *F AE003603 22645 .. *F 22646 *F AE003603 23273 *F .........-....g.......................... 23312 *F AE003212 1283 .. *F 1284 *F AE003843 210011 .. *F 210010 *F AE003843 311840 *F .......c.........-....g.......................... 311793 *F AE003843 301184 *F .......c........t-............................... 301231 *F AE003843 312276 ... *F 312274 *F AE003395 19484 ... *F 19482 *F AE003389 9836 *F .......c.........-...cg.......................... 9883 *F AE003090 22207 .. *F 22206 *F AE003090 21614 *F .......c.........-......................t........ 21567 *F AE003605 264594 .. *F 264593 *F AE003605 263955 *F .....t...-............................... 263916 *F AE003815 92493 .. *F 92492 *F AE002772 1269 .......c.........-....g................... *F 1309 *F AE002837 19949 *F .......c.........-............................... 19996 *F AE003787 211601 *F ....c.........-............................... 211645 *F AE002942 38 .........-............................... 77 *F AE003574 207863 *F .......c.........-............................. 207818 *F AE003147 11656 *F .......ac.....a..-...........ta................c. 11609 *F AE003573 192944 .......a.........-............ *F 192972 *F AE003638 84621 ..............t..a...... *F 84598 *F AE003445 181388 \-.................. *F 181371 *F AE003559 55830 .......-........... *F 55847 *F AE003796 202104 .................. *F 202087 *F AE003656 122170 \-..............a...... *F 122190 *F AE003650 35640 .-................ *F 35624 *F AE003608 216066 ............ *F 216077 *F AE003510 192021 ............ *F 192010 *F AE003506 201082 ............. *F 201094 *F AE003499 5553 ................. *F 5569 *F AE003828 207899 ...-.............. *F 207915 *F AE003599 238297 ............g........ *F 238317 *F AE003772 101930 \-.......t............. *F 101950 *F tmpseq_0 583 att 585 *F AE003788 122472 ... 122474 *F AE003040 41843 ... 41845 *F AE003066 2462 ... 2460 *F AE003786 25694 ... 25692 *F AE003512 298746 ... 298748 *F AE002582 9610 ... 9608 *F AE003845 86346 ... 86344 *F AE003846 37925 ... 37927 *F AE003603 23313 ... 23315 *F AE003843 311792 ... 311790 *F AE003843 301232 ... 301234 *F AE003389 9884 ... 9886 *F AE003090 21566 ... 21564 *F AE003605 263915 ... 263913 *F AE002837 19997 ... 19999 *F AE003787 211646 ... 211648 *F AE002942 78 ... 80 *F AE003147 11608 ... 11606 # *U FBrf0135200 *a Levis *b R. *t 2001.2.6 *T personal communication to FlyBase *u FlyBase error report on Tue Feb 6 15:08:11 2001. *F From levis@ciwemb.edu Tue Feb 06 20:07:28 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 6 Feb 2001 20:07:28 \+0000 *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Tue, 6 Feb 2001 15:08:11 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: EP(2)0638 is inserted in CG8415 intron *F Cc: Allan Spradling *F The P element insertion in EP(2)0638 has been mapped by in situ *F hybridization to 50E4-7. A BLAST of the 340 nt flanking sequence *F shows that the 3' portion (beginning at nt 117) is a repetitive *F sequence. This explains why the insertion has not been localized on *F the genomic sequence on GeneSeen. However, the 5' portion of the *F flanking sequence (1-117) shows significant sequence similarity to *F only one segment of the genomic sequence and thus allows the *F insertion to be mapped on the genomic sequence. The entire flanking *F sequence aligns perfectly with the scaffold segment AE003815, as *F shown below. *F >gb|AE003815.2|AE003815 Drosophila melanogaster genomic scaffold *F >142000013386047 section 28 of *F 52, complete sequence *F Length = 240495 *F Score = 668 bits (337), Expect = 0.0 *F Identities = 337/337 (100%) *F Strand = Plus / Plus *F Query: 1 gcgccttatcaataaaaaacgcaaatttgtcgcacggcccgattggtcgatgcgatagaa 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 92130 gcgccttatcaataaaaaacgcaaatttgtcgcacggcccgattggtcgatgcgatagaa 92189 *F Query: 61 acgaaactaaaagcggaattgacccgaaacgatatcgaatatccatggtcgtattgtaca 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 92190 acgaaactaaaagcggaattgacccgaaacgatatcgaatatccatggtcgtattgtaca 92249 *F Query: 121 gttgcggtaacaataatagcaccataagcacatttcgtgtttggcccaccgtttctgtat 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 92250 gttgcggtaacaataatagcaccataagcacatttcgtgtttggcccaccgtttctgtat 92309 *F Query: 181 tttctaacactttttcatcatattgctgactaaacttaaatactacagtgatttgctcgt 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 92310 tttctaacactttttcatcatattgctgactaaacttaaatactacagtgatttgctcgt 92369 *F Query: 241 gtgttttgagtcgttatcctgttgaaatgtccaaaccaacggcatttcatcctcggcata 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 92370 gtgttttgagtcgttatcctgttgaaatgtccaaaccaacggcatttcatcctcggcata 92429 *F Query: 301 tggcagcatcagattttccaggatatctgtgtaaatg 337 *F ||||||||||||||||||||||||||||||||||||| *F Sbjct: 92430 tggcagcatcagattttccaggatatctgtgtaaatg 92466 *F I believe that this insertion position maps to the intron of CG8415. *F The estimated cytogenetic location of this gene (50E6) is consistent *F with the in situ localization of EP(2)0638. There are no other known *F mutant alleles of CG8415, according to the FlyBase report. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210 *F phone: (410) 554-1238 *F fax: (410) 467-1147 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0135201 *a Levis *b R. *t 2001.2.19 *T personal communication to FlyBase *u FlyBase error report on Mon 19 Feb 17:07:52 2001. *F From levis@ciwemb.edu Mon Feb 19 22:07:01 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Feb 2001 22:07:02 \+0000 *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Mon, 19 Feb 2001 17:07:52 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: l(2)k08316 not mapped in GeneSeen *F The P element insertion line flanking sequence maps unambiguously *F (100% match 388 nt) to a site in the px gene, but it is not mapped on *F GeneSeen in this region. According to Spradling et al. 1999, this *F strain is part of the primary collection. I think there is a stock *F for it in the Bloomington stock center. *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210 *F phone: (410) 554-1238 *F fax: (410) 467-1147 *F email: levis@ciwemb.edu *F \------------------------------------ *F From sima@fruitfly.bdgp.berkeley.edu Thu Feb 22 19:36:59 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Feb 2001 19:36:59 \+0000 *F Date: Thu, 22 Feb 2001 11:36:39 \-0800 (PST) *F From: Sima Misra *F X-Sender: sima@fruitfly *F To: Robert Levis , *F Guochun Liao , *F Madeline Crosby *F cc: flybase-help@morgan.harvard.edu *F Subject: Re: l(2)k08316 not mapped in GeneSeen (fwd) *F MIME-Version: 1.0 *F Hi Bob, Guochun, and Lynn, *F I'm glad we've sorted out that the sequence from the line is chimeric. ... *F Bob, I tried BLASTing at NCBI also and have no clue what that DNA might be *F from! *F Best wishes, *F sima *F > Sima & Guochun, *F > *F > Sorry for the misinformation about the % identity in the alignment *F > with genommic. I must have been looking at the alignment of the *F > insertion sequence with itself rather than the genomic sequence. Out *F > of curiosity, I tried BLASTing just the sequence that doesn't align *F > well with genomic (312-388) against all Drosophila sequences and got *F > no good hits. I wonder where it comes from? *F > *F > ...Bob *F > *F > *F > >Hi Sima, *F > >This element l.2.k08316 only has 311/388 bases mapped to genomic sequence *F > >both release 1 and 2. The automatic analysis thought it's a chimeric and *F > >left it out. The insertion site should be 2R, 17451994, cytological *F > >location 58E5. *F > > *F > >-guochun *F > > *F > >On Tue, 20 Feb 2001, Sima Misra wrote: *F > > *F > >> *F > >> Hey Guochun, *F > >> *F > >> could you look this one up please? *F > >> *F > >> Many thanks, *F > >> sima *F > >> *F > >> \---------- Forwarded message \---------- *F > >> Date: Mon, 19 Feb 2001 17:07:52 \-0500 *F > >> From: Robert Levis *F > >> To: flybase-help@morgan.harvard.edu *F > >> Subject: l(2)k08316 not mapped in GeneSeen *F > >> *F > >> The P element insertion line flanking sequence maps unambiguously *F > >> (100% match 388 nt) to a site in the px gene, but it is not mapped on *F > >> GeneSeen in this region. According to Spradling et al. 1999, this *F > >> strain is part of the primary collection. I think there is a stock *F > >> for it in the Bloomington stock center. *F > >> \-- *F > >> \------------------------------------ *F > >> Robert W. Levis, Ph.D. *F > >> Carnegie Institution of Washington *F > >> Department of Embryology *F > >> 115 West University Parkway *F > >> Baltimore, MD 21210 *F > >> *F > >> phone: (410) 554-1238 *F > >> fax: (410) 467-1147 *F > >> email: levis@ciwemb.edu *F > >> \------------------------------------ *F > >> *F > # *U FBrf0135208 *a Salecker *b I. *t 2001.3.5 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Mon Feb 26 16:40:02 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 26 Feb 2001 16:40:02 \+0000 *F To: zipursky@hhmi.ucla.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Mon, 26 Feb 2001 16:40:03 \+0000 *F Content-Length: 1330 *F Dear Dr. Zipursky, *F I am curating your paper for FlyBase: *F Poeck et al., 2001, Neuron 29(1): 99--113 *F and I have a question about the 'not2' allele ( == superscript). *F >From what you said in the paper I think that this is probably an allele *F obtained from the Berkeley Drosophila Genome Project, since you *F reference the paper: Spradling et al., 1999, Genetics 153(1): 135--177. *F I would be grateful if you could tell me the designation of the lethal *F line for this allele e.g. l(2)01642, since at the moment I think we *F must have information for the not2 allele under 2 separate allele *F records in FlyBase \- both under 'not2' and also separately under *F whichever Berkeley lethal it is. I would like to be able to merge them *F so that all the data is in one place in the database. I would record *F any information you give me about this line as a personal communication *F from you to FlyBase, *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From isaleck@nimr.mrc.ac.uk Mon Mar 05 13:13:13 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 5 Mar 2001 13:13:13 \+0000 *F Mime-Version: 1.0 *F X-Sender: isaleck@pophost *F Date: Mon, 5 Mar 2001 13:11:25 \+0000 *F To: gm119@gen.cam.ac.uk *F From: Iris Salecker *F Subject: not2 *F Dear Gillian, *F Dr. Zipursky forwarded me your message regarding the not2 allele. *F Please find below the information about not2. This information has *F been sent to me by Dr. B. Poeck, University of Regensburg, who *F isolated the different nonstop alleles. *F Stock Number: 11553 *F Old P Stock#: P1553 *F Genotype: P{ry+t7.2=PZ}l(3)0206902069 ry506/TM6B, ryCB Tb+ *F Chromosome(s): 3 *F Breakpts/Insertion: 075C03-04 *F Date added: 6/01/93 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Allan Spradling *F Please do not hesitate to contact us, if you would need further information. *F With best wishes, *F Iris *F \-- *F Iris Salecker (Ph.D.) *F Division of Molecular Neurobiology *F National Institute for Medical Research *F The Ridgeway *F Mill Hill *F London, NW7 1AA *F United Kingdom *F Phone: \+44 (0) 208 959 3666 (ext. 2601) *F FAX: \+44 (0) 208 913 8536 *F e-mail: isaleck@nimr.mrc.ac.uk # *U FBrf0135234 *a Treinin *b M. *t 2001.3.14 *T personal communication to FlyBase *u FlyBase error report for CG9349 on Wed Mar 14 03:46:33 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Mar 14 11:46:40 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 14 Mar 2001 11:46:40 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 14 Mar 2001 03:46:33 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: millet_t@cc.huji.ac.il *F Subject: FlyBase error report for CG9349 on Wed Mar 14 03:46:33 2001 *F Content-Length: 577 *F Error report from Millet Treinin (millet_t@cc.huji.ac.il) *F Gene or accession: CG9349 *F Release: 2 *F Missed gene *F Comments: The sequence of CG9349 as shown in your first release shows both *F sequence homology *F and structureal homology to C. elegans t14a8.1. In your new release the *F predicted *F protein is much shorter, thus eliminating a coiled coil region which is part of *F the structural similarity with the nematode gene. Thus I believe that the *F previous *F prediction should be maintained. *F Browser: Mozilla/4.01 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135235 *a Schlenke *b T.A. *t 2001.3.12 *T personal communication to FlyBase *u FlyBase error report for CG5923 on Mon Mar 12 16:14:13 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Mar 13 00:14:16 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 13 Mar 2001 00:14:16 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 12 Mar 2001 16:14:13 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: taschlenke@ucdavis.edu *F Subject: FlyBase error report for CG5923 on Mon Mar 12 16:14:13 2001 *F Content-Length: 1196 *F Error report from Todd A. Schlenke (taschlenke@ucdavis.edu) *F Gene or accession: CG5923 *F Release: 1 *F Gene annotation error *F Gene CG5923 has incorrect exon/intron structure or translation start site. *F Comments: I believe there is a mistake in the predicted ORF for the gene *F DNApol-alpha73 (CG5923) of accession AE003760. The start codon appears to be *F several AAs downstream of the predicted start codon. *F There are two reasons I believe this is so. One, the 5' EST (LD41453.5prime) *F for this gene starts downstream of the predicted start. Second, the *F homologous sequence in Drosophila simulans lines (unpublished) shows the *F predicted start codon to be polymorphic and that there is a frameshift *F mutation directly after the predicted start codon. *F There are two in-frame ATG codons near the beginning of the 5' EST sequence *F (at AAs 45 and 56 of the predicted gene), both of which start long open *F reading frames in melanogaster and simulans. My guess is the second ATG codon *F (at AA 56, such that the gene starts MGVEP...) is the real start codon, based *F on its distance from the start of the 5' EST sequence. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135236 *a Ashburner *b M. *t 2001.3.12 *T personal communication to FlyBase *u FlyBase error report for CG1474 on Mon Mar 12 09:03:44 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Mar 12 17:03:50 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 12 Mar 2001 17:03:50 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 12 Mar 2001 09:03:44 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG1474 on Mon Mar 12 09:03:44 2001 *F Content-Length: 561 *F Error report from ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG1474 *F Release: 1 *F Missed gene *F Comments: It worries me that there are three 'genes' all at the same place *F and all with predictions of being sepiapterine reductase *F \*a Es2 *F \*i CG1474 *F \*m SPTREMBL:O44424 *F \*m SPTREMBL:Q9W3F2 *F \*a Sptr *F \*m SPTREMBL:O76752 *F \*i CG12117 *F \*a CG12116 *F \*m SPTREMBL:Q9W3F1 *F The proteins have no overlap and I have not delved further but a *F (re)-curator should :=)) michael *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135237 *a Misra *b S. *t 2001.3.5 *T personal communication to FlyBase *u FlyBase error report for CG15111 on Mon Mar 5 14:24:55 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Mar 05 22:25:04 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 5 Mar 2001 22:25:04 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 5 Mar 2001 14:24:55 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG15111 on Mon Mar 5 14:24:55 2001 *F Content-Length: 1002 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG15111 *F Release: 2 *F Gene annotation error *F Gene CG15111 has incorrect exon/intron structure. *F Protein sequence: *F >CG15111 with corrected start site of translation *F MLFTRRRLLKRVGLRCLQACLLIFFLIFVVLPLIFRYSVTFQRGILFLT *F FIKYPKGLDLTKPESVGLYATRNFYITVKDHDQDEDGVRVGVWHVLPSN *F AVRRFKRELRVEEEVAQDPDQQLDPAPGNERELKELSPAIRSEFPVVLP *F ENEQLFYERLLRMPGGTVVLYLHGNTASRGSGHRSEVYKLLRKLNYHVF *F SFDYRGYADSDPVPPTEEGVVRDAMMVFEYIANTTSNPIVVWGHSLGTG *F VATHLCAKLASLRERAPRGVILESPFTNIRDEIRMHPFAKLYKNLPWFN *F FTISQPMYTNRLRFESDVHVLEFRQPIMIIHAEDDVVVPFNLGYRLYRI *F ALDGRSRTSGPVEFHRFGASRKYGHKYLCRAPELPGLIQKFVENYRDAV *F Y *F Comments: An error in setting the correct start of translation resulted in a *F frameshift. *F The resulting protein should be 393aa instead of 36aa. *F The new protein shows sequence similarity to accessions AAH02138, T17237, *F CAB99288.1, and AAH02263 by BLASTP. *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.8 sun4u; Nav) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135264 *a Dickson *b B. *t 2001.3.14 *T personal communication to FlyBase *u *F From dickson@nt.imp.univie.ac.at Wed Mar 14 21:19:53 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 14 Mar 2001 21:19:53 \+0000 *F Mime-Version: 1.0 *F Date: Wed, 14 Mar 2001 22:25:04 \+0100 *F To: Gillian Millburn (Genetics) *F From: Barry Dickson *F Subject: Re: FlyBase query *F Dear Gillian, *F We've just noticed a minor inconsistency in our Robo2/3 paper (Rajagopalan *F et al. Cell 103:1033). The robo2 ORF has an extra 57 codons before what is *F most likely the start methionine. Without these extra amino acids, the *F predicted protein (incl. signal sequence) is 1406 AA. This is the length we *F give in the paper, and also the length of the protein sequence we deposited *F in GenBank together with the Goodman lab. However, the full open reading *F frame encodes a protein of 1463 AAs, and unfortunately this is the ORF we *F used to assign the mutations we detected in each of the robo2 alleles, as *F listed in Table 1. *F Here are the molecular details of each allele based on amino acid positions *F in the 1406 AA protein: *F robo21: R384->Stop *F robo22: R444->Stop *F robo23: W455->Stop *F robo24: W578->Stop *F robo25: W666->Stop *F robo26: K731->Stop *F robo27: W825->Stop *F robo28: R845->Stop *F robo29: S120->F *F robo210: G158->E *F Would you be able to include some comment to this effect when you add the *F allele information into FlyBase. *F Thanks once again for your help. *F Best wishes, *F Barry *F .. *F \-------------------------------------------------- *F Barry Dickson *F Research Institute of Molecular Pathology (I.M.P.) *F Dr. Bohr-Gasse 7 *F A-1030 Vienna *F Austria *F Tel. \+43 1 797 30 421 *F Fax. \+43 1 798 71 53 *F Email. dickson@nt.imp.univie.ac.at *F \-------------------------------------------------- # *U FBrf0135265 *a Endow *b S.A. *t 2001.4.14 *T personal communication to FlyBase *u *F From endow001@mc.duke.edu Sat Apr 14 00:13:12 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 14 Apr 2001 00:13:12 \+0100 *F Date: Fri, 13 Apr 2001 19:08:01 \-0400 *F From: sharyn a endow *F Reply-To: endow001@mc.duke.edu *F Organization: duke university *F X-Mailer: Mozilla 4.5 (Macintosh; I; PPC) *F X-Accept-Language: en,pdf *F MIME-Version: 1.0 *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query: kinesins *F Content-Transfer-Encoding: 7bit *F The genes can be identified by finding the sequence in the alignment and *F correlating with the database entry: *F DmKlp53D DmCG8566 *F DmKlp31D DmCG5300 (same as DmKlp31E) \- this was thought to be a *F separate gene at the time the tree was built *F DmKlp10A DmCG1453 *F DmKlp59C DmCG3219 *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Sharyn A. Endow, PhD *F Professor of Microbiology *F Duke University Medical Center *F 438 Jones Building/Research Drive *F PO Box 3020 *F Durham, NC 27710 *F T 919 684-4311 F 919 684-8735 *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F > I am curating your paper and poster on kinesins from J. Cell Science *F > (Kim and Endow, 2000, J. Cell Sci. 113(21): 3681--3682) for FlyBase and *F > I have a couple of questions. *F > *F > I am trying to track down which genes in FlyBase the various kinesins *F > on the poster correspond to (so that we do not end up with duplicate *F > entries in the database). I found the link to the 'Kinesin Home Page' *F > (http://blocks.fhcrc.org/~kinesin/) on your web site, which was very *F > useful, but there are still 4 names that are on your poster which are *F > not in the Table which gives the accession numbers of the various *F > kinesins (http://blocks.fhcrc.org/~kinesin/OrgTables/drome.html). *F > *F > I include a Table of the 4 genes from your poster below which I am not *F > sure which gene in FlyBase they correspond to. I have listed what the *F > gene was called in the poster and which subfamily it is in, and then in *F > a separate column, which gene in FlyBase I think they might correspond *F > to (based on cytology). *F > *F > Gene symbol in J. Cell Sci. | gene in FlyBase I think it might correspond *F > to | *F > \---------------------------------------------------------------------------- *F --- *F > *F > DmKlp53D | unc-104 (also called CG8566) ? *F > | *F > (Unc104/KIF1 family) | accession number: AF247761 *F > | *F > \---------------------------------------------------------------------------- *F --- *F > *F > DmKlp31D | ? *F > | *F > (Chromokinesin/KIF4 family) | *F > | *F > \---------------------------------------------------------------------------- *F --- *F > *F > DmKlp10A | CG1453 ? *F > | *F > (MCAK/KIF2 family) | *F > | *F > \---------------------------------------------------------------------------- *F --- *F > *F > DmKlp59C | CG3219 or CG12192 ? *F > | *F > (MCAK/KIF2 family) | *F > | *F > \---------------------------------------------------------------------------- *F --- *F > *F > I would be grateful if you could confirm whether I am correct, and also *F > tell me whether 'DmKlp31D' corresponds to any gene in FlyBase, *F > *F > I look forward to hearing from you, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- # *U FBrf0135266 *a Koc *b E. *t 2001.3.8 *T personal communication to FlyBase *u *F From eminekoc@email.unc.edu Thu Mar 08 15:39:07 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Mar 2001 15:39:07 \+0000 *F Date: Thu, 8 Mar 2001 10:39:08 \-0500 (EST) *F From: Emine Koc *F X-Sender: eminekoc@login0.isis.unc.edu *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='-1744699039-75330165-984065948=:73414' *F Content-Length: 97134 *F Dear Dr. Millburn, *F .. *F Yes that is right.Some portion of Drosophila S 14 is embedded in the *F N-terminus of CG12211 gene product with protein id AAF48956. However, *F very beginning of the N-terminus, we found in genomic scaffold AE003512, *F is missing, and the C-terminus of the sequence which is embedded in the *F CG12211 is not correct. *F If you take the complement of the sequences between 81476 and 81162 and *F translate it, you would get the correct drosophila S14 protein. However, *F the first methionine is still missing in this sequence, we were not able *F to find it. *F I am attaching the drosophila S14 protein sequence and also the alignment *F of drosphila S14 with yeast,human,worm mitochondrial S14s.I hope this will *F be helpful. If you have further question, please feel free to ask. *F Emine *F Mitochondrial S14 Homologs *F drosop 1 ~~~~~~~~~~~~~~~~~~~~~~~QVRTKYADWKMIRDVKRRKCVKENAVERLRVNSLRKN *F Human 1 MAAFMLGSLLRTFKQMVPSSASGQVRSHYVDWRMWRDVKRRKMAYEYADERLRINSLRKN *F worm 1 ~~~~~~~~~~~~~~~~~~~~~~~~~MTFWASWRQLRDVKRREQIQEVGADRMRLKAIKFN *F yeast 1 ~~~~~~~~~~~~~MGNFRFPIKTKLPPGFINARILRDNFKRQQFKENEILVKSLKFIARN *F drosop 38 DILPPELREVADAEIAAFPRDSSLVRVRERCALTSRPRGVVHKYRLSRIVWRHLADYNKL *F Human 61 TILPKILQDVADEEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQL *F worm 36 TILPQAIRDEAAEKMQKARKYDHPRLILNMCQFTGRQRGKIKPYRLSRHLFRRFADRSAL *F yeast 48 MNLPTKLRLEAQLKLNALPNYMRSTQIKNRCVDSGHARFVLSDFRLCRYQFRENALKGNL *F drosop 98 SGVQRAMW *F Human 121 SGIQRATW *F worm 96 SGVQRAMW *F yeast 108 PGVKKGIW *F > > 'Gillian Millburn (Genetics)' wrote: *F > > *F > > Dear Dr. Spremulli, *F > > *F > > I am curating your paper for FlyBase: *F > > *F > > Koc et al., 2000, J. Biol. Chem. 275(42): 32585--32591 *F > > *F > > and I have a question about the 'MRP-S14' homolog in Drosophila you *F > > describe in the paper. *F > > *F > > In your paper you say: *F > > *F > > 'The D. melanogaster genome also has a putative MRP-S14 homolog *F > > (AE003512). However, due to a probable sequencing error, the D. *F > > melanogaster homolog could not be obtained from the given coding *F > > sequence. Instead, the partial putative S14 sequence is embedded in a *F > > long open reading frame of unknown function (CG12211).' *F > > *F > > I think from this paragraph that you are saying that the CG12211 open *F > > reading frame as it is currently annotated contains partial S14 *F > > sequence, and also (either side of the S14 sequence) unrelated exons, *F > > so that CG12211 needs splitting in two \- to create an S14 gene and a *F > > gene made of the surrounding unrelated exons. Is this interpretation *F > > correct ? *F > > *F > > Also, I would be grateful if you could send me the part of the sequence *F > > of CG12211 that you think corresponds to the S14 homolog. I would *F > > curate this information as a personal communication from you to *F > > FlyBase, and then it would be used by the molecular curators at FlyBase *F > > when they reannotate this part of the genome (they are systematically *F > > going to work through the genome to improve the 'CG' annotations based *F > > on information from sequence databases, papers and personal *F > > communications to FlyBase, so that the intron/exon structures of the *F > > annotations are improved and the various splice forms are included in *F > > the annotations). Any information you have on the likely sequence and *F > > intron/exon structure of the Drosophila S14 homologue would be very *F > > useful. *F > > *F > > I look forward to hearing from you, *F > > *F > > Gillian *F > > *F > > \-------------------------------------------------------------- *F > > Gillian Millburn. *F > > *F > > FlyBase (Cambridge), *F > > Department of Genetics, *F > > University of Cambridge, *F > > Downing Street, email: gm119@gen.cam.ac.uk *F > > Cambridge, CB2 3EH, Ph : 01223-333963 *F > > UK. FAX: 01223-333992 *F > > \-------------------------------------------------------------- # *U FBrf0135267 *a Papatsenko *b D. *t 2001.4.18 *T personal communication to FlyBase *u *F From dap5@is2.nyu.edu Wed Apr 18 17:36:32 2001 *F Content-Type: multipart/mixed; boundary='=_AABklwAAafA63c0c' *F Content-Length: 8340 *F Hi Gillian, this is reply to your request concerning rh7: *F CG5638 seems to be correct. Here is the result of rh7 search in BDGP: *F >CG5638|FBgn0036260|CT17820|FBan0005638 last_updated:000321 *F Length = 2177 *F Plus Strand HSPs: *F Score = 1273 (191.0 bits), Expect = 2.5e-52, P = 2.5e-52 *F Identities = 259/263 (98%), Positives = 259/263 (98%), Strand = Plus / Plus *F Query: 1 AAATATTTGGCCAAGTCAGTCATGGAGGCCATCATCATGACGACCCTGCCCAACCTGACA 60 *F AAATATTTGGCCAAGTCAGTCATGGAGGCCATCATCATGACGACCCTGCCCAACCTGACA *F Sbjct: 705 AAATATTTGGCCAAGTCAGTCATGGAGGCCATCATCATGACGACCCTGCCCAACCTGACA *F 764 *F Query: 61 ACGGATGCAGGTGACAGCAGCTTCTGGCTAACCGGTGCCCTCTCGCTCTCCGAGATGTTG *F 120 *F ACGGATGCAGGTGACAGCAGCTTCTGGCTAACCGGTGCCCTCTCGCTCTCCGAGATGTTG *F Sbjct: 765 ACGGATGCAGGTGACAGCAGCTTCTGGCTAACCGGTGCCCTCTCGCTCTCCGAGATGTTG *F 824 *F Query: 121 GCCAACTCGAGCCACAGCCATTCCACTGGGAGCACAACCTCGACGGCTGGGAGTTCGGCC *F 180 *F GCCAACTCGAGCCACAGCCATTCCACTGGGAGCACAACCTCGACGGCTGGGAGTTCGGCC *F Sbjct: 825 GCCAACTCGAGCCACAGCCATTCCACTGGGAGCACAACCTCGACGGCTGGGAGTTCGGCC *F 884 *F Query: 181 ACCGAATCATCCGCTGTGAATGTAGGCAAGGACCACGACAAGCACGTGAATGACAGCGTT *F 240 *F ACCGAATCATCCGCTGTGAATGTAGGCAAGGACCACGACAAGCACGTGAATGACAGCGTT *F Sbjct: 885 ACCGAATCATCCGCTGTGAATGTAGGCAAGGACCACGACAAGCACGTGAATGACAGCGTT *F 944 *F Query: 241 TCCACGGGTCTGAGGTAAGTGCA 263 *F TCCACGGGTCTGAG AA T CA *F Sbjct: 945 TCCACGGGTCTGAGC-AATTACA 966 *F At 09:43 AM 4/18/01 \-0700, you wrote: *F >Dmitri: *F > *F >Could you please confirm? *F >Thanks, *F >Claude *F > *F >Dear Dr. Desplan, *F > *F >I am curating your paper for FlyBase: *F > *F >Papatsenko et al., 2001, Mech. Dev. 101(1-2): 143--153 *F > *F >and I have a question about one of the genes shown in Figure 2 \- rh7. *F > *F >We don't have a record of rh7 in FlyBase, but I think it may be one of *F >the predicted CG genes \- CG5638, from the information we have about *F >this CG. I would be grateful if you could confirm whether I am *F >correct, *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- # *U FBrf0135268 *a Campbell *b P. *t 2001.2.6 *T personal communication to FlyBase *u FlyBase error report for CG8425 on Tue Feb 6 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 07 03:40:41 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 03:40:41 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 6 Feb 2001 19:40:22 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: peterca@ento.csiro.au *F Subject: FlyBase error report for CG8425 on Tue Feb 6 19:40:22 2001 *F Content-Length: 336 *F Error report from Peter Campbell (peterca@ento.csiro.au) *F Gene or accession: CG8425 *F Gene annotation error *F Gene CG8425 corresponds to AF304352 (FBgn) *F Comments: GenBank entry for our cDNA sequence will be released in a few days. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 07 03:46:48 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 03:46:48 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 6 Feb 2001 19:46:39 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: peterca@ento.csiro.au *F Subject: FlyBase error report for CG8425 on Tue Feb 6 19:46:39 2001 *F Content-Length: 630 *F Error report from Peter Campbell (peterca@ento.csiro.au) *F Gene or accession: CG8425 *F Gene annotation error *F Gene CG8425 has incorrect exon/intron structure. *F Comments: From our cDNA sequence, the intro-exon structure is correct except *F that the sixth predicted intron is not removed; instead there is a stop codon *F precisely where the intron was predicted followed by a poly adenylation *F signal and a poly A tail. The sequence goes: CTC AAC GAA AAG TAA encoding *F LNEKstop. Following predicted exons are not part of the gene. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 07 04:07:37 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 04:07:37 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 6 Feb 2001 20:07:27 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: peterca@ento.csiro.au *F Subject: FlyBase error report for CG8425 on Tue Feb 6 20:07:27 2001 *F Content-Length: 553 *F Error report from Peter Campbell (peterca@ento.csiro.au) *F Gene or accession: CG8425 *F Gene annotation error *F Gene CG8425 should be split. *F Comments: The N-terminal 2/3 of the predicted product is actually the complete *F product which is an esterase. We have not looked closely at the last 1/3 but *F your annotation shows it has a motif that is not part of an esterase. Our *F cDNA corresponds to the N-terminal 2/3 that contains the esterase motifs. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 07 04:13:10 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 04:13:10 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 6 Feb 2001 20:13:01 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: peterca@ento.csiro.au *F Subject: FlyBase error report for CG8425 on Tue Feb 6 20:13:01 2001 *F Content-Length: 603 *F Error report from Peter Campbell (peterca@ento.csiro.au) *F Gene or accession: CG8425 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG8425. *F Comments: The function of this gene is Juvenile Hormone Esterase (JHE). JHE *F was purified (Campbell et al, 1998, Insect Biochem. Mol. Biol., 28, 501-515) *F and the peptide mass fingerprint corresponds to N-terminal 2/3 of the *F predicted product of CG8425 (Campbell et al., 2001, Insect Biochem. Mol. *F Biol., in press) *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135269 *a Campbell *b P. *t 2001.2.6 *T personal communication to FlyBase *u FlyBase error report for CG8424 on Tue Feb 6 20:22:17 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Feb 07 04:22:39 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Feb 2001 04:22:39 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 6 Feb 2001 20:22:17 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: peterca@ento.csiro.au *F Subject: FlyBase error report for CG8424 on Tue Feb 6 20:22:17 2001 *F Content-Length: 364 *F Error report from Peter Campbell (peterca@ento.csiro.au) *F Gene or accession: CG8424 *F Missed gene *F Comments: CG8424 is most similar to CG8425, the adjacent gene so it appears to *F be a duplication of CG8425. CG8425 is identified as juvenile hormone esterase. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135270 *a Campbell *b P. *t 2001.2.8 *T personal communication to FlyBase *u FlyBase error report for CG9858 on Wed Feb 7 20:59:31 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Feb 08 13:27:17 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 8 Feb 2001 13:27:17 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 7 Feb 2001 20:59:31 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: peterca@ento.csiro.au *F Subject: FlyBase error report for CG9858 on Wed Feb 7 20:59:31 2001 *F Content-Length: 735 *F Error report from Peter Campbell (peterca@ento.csiro.au) *F Gene or accession: CG9858 *F Release: 2 *F Gene annotation error *F Gene CG9858 has a mistake in the supporting evidence or functional assignment. *F Comments: This gene does not correspond to the JHE reported in Campbell et al. *F 1992 or 1998. That protein was purified in the 1998 paper and that material *F was used to generate a peptide mass fingerprint which unequivocally identifies *F CG8425. This identification of JHE is in press with Insect Biochem. Mol. *F Biol. Claudianos et al. merely noted that CG9858 has some sequence *F similarities to other JHEs and called it JHE-related. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows NT) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0135271 *a Weir *b M. *t 2001.3.11 *T personal communication to FlyBase *u FlyBase error report for CG9952 on Sun Mar 11 06:57:55 2001. *F From FlyBase-error@hedgehog.lbl.gov Sun Mar 11 14:58:02 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 11 Mar 2001 14:58:02 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 11 Mar 2001 06:57:55 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mweir@wesleyan.edu *F Subject: FlyBase error report for CG9952 on Sun Mar 11 06:57:55 2001 *F Content-Length: 482 *F Error report from Michael Weir (mweir@wesleyan.edu) *F Gene or accession: CG9952 *F Release: 1 *F Gene annotation error *F Gene CG9952 has a mistake in the supporting evidence or functional assignment. *F Comments: It encodes a putative F-box receptor for ubiquitin-mediated protein *F degradation. The Paired protein is one of its putative substrates for *F degradation (see primary ref. 2). *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135272 *a Wilson *b I. *t 2001.3.22 *T personal communication to FlyBase *u FlyBase error report for CG17771 on Thu Mar 22 08:19:18 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 22 16:19:23 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Mar 2001 16:19:23 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 22 Mar 2001 08:19:18 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: iwilson@edv2.boku.ac.at *F Subject: FlyBase error report for CG17771 on Thu Mar 22 08:19:18 2001 *F Content-Length: 439 *F Error report from Iain Wilson, Univ. f. Bodenkultur, Wien, Austria *F (iwilson@edv2.boku.ac.at) *F Gene or accession: CG17771 *F Release: 1 *F Missed gene *F Comments: The protein encoded by CG17771 appears in fact to be most homologous *F to the recently-cloned human xylosyltransferase (AJ277442) required for *F biosynthesis of the proteoglycan core (EC 2.4.2.26). *F Browser: Mozilla/4.75 en (Win98; U) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135273 *a Kitagawa *b Y. *t 2001.3.22 *T personal communication to FlyBase *u FlyBase error report for CG7002, hemolectin(hml), AB035891 on Wed Mar 21 05:19:43 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 22 16:22:00 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 22 Mar 2001 16:22:00 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 21 Mar 2001 05:19:43 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: yasuok@nuagr1.agr.nagoya-u.ac.jp *F Subject: FlyBase error report for CG7002, hemolectin(hml), AB035891 on Wed Mar *F 21 05:19:43 2001 *F Content-Length: 17120 *F Error report from Yasuo Kitagawa (yasuok@nuagr1.agr.nagoya-u.ac.jp) *F Gene or accession: CG7002, hemolectin(hml), AB035891 *F Release: 1 *F cDNA or EST error *F Gene cDNA sequence: *F AGTGCTAAGTGTAAAAGTTTTTAAAAGTGAGTTCTAAGCCGGATTTTAGACAGGACTAACAGCTTGGCAGTGTCCTTGC *F AGAAATGGATTACCAGTGCTAACAATCAACAGGATTTTCCTGTATATACAATTAAACCAACAATTTCCGATTAGCCTAA *F CAAAATGGCTAACAAAGTGATTTTTGTGGCGTTGTGGCTGCTTTTTATTTCATCTGTTTTGACAGAGAATGGAGACATT *F ATAATAACCGATGATGACGACGAGGATATCAACCCGCTGCTCCAGGCCGCACCGGAAGTCGTCGATAAGGAGAACGAAC *F GGAACGAACGCCACATTAGCTTCAACATCCTCAAAGCCCCAGCAGTTAGTGCACGATACACTTATGGGGGTTCTGAAGG *F AGGCCGTAGTGGATTCGGAGGAGGATATGGTGTTGGTGGAGGTGGAGGCGGTGGTTATGGCGGGATAAAGACGTCTCAT *F GCCGCTGGAGGACTGCTTACCAAAACCTTGGGCTTTTTAGGCATGGGTGGCCAGGGAAGTCAGGGCAACTTTTACGGAG *F GCGGAGGACACTTTCTAGCAGCCTTCGGAAAATGTTCAGCCCTCCAACTGCCGTCCAATGTTCAGTCTGAATGCCACAA *F TGGTCGTTGCAAAGTCTTCTGCCCAACTGGCTATAGCTTCGCCCAGGACGTTTCAGTCCTGGAGATGTTCTGCAGCAAT *F GATGGATGGATCATAGGCAACTCCGTGTTTGCGGAGGTGCCACCCTGCCAGGCACAATGTACACCTCCATGCCAAAACA *F ATGGCATCTGCATCTCCGCCGGAGTGTGCCAGTGTCCAGAGAACTACTACGGTCCGCTGTGTCAGCAAAAGAAGTCCAT *F ATGTGCATCCTTTCCGAAGGCACCGAAGAACTCCAAAGTTTCCTGCAAGAACAATATGTGCCACGCGGAATGCATGAGA *F GGATTCCAGTTTCCAGACGGCAGCGGAATTACGAACATTGA! *F GTGCCGCAATGGCCAGTGGGTGCACACAAAAACTGGGCTATCCAAAACTCCAGATTGTGCTCCTACTTGTGCGCCCGCC *F TGCCAAAACGGAGGTCAGTGCATTAGTTTCAATGTCTGTCAGTGCTCTAAGATGTTCCGCGGAGACCATTGTCAATATA *F ACATTGACAGATGCAATGTGACTAATACAAATTTTAATGGAAACTACAAGTGTGCCTACGAAATGGACGACGCTCGTTG *F CACATTTAGTTGTCCTCAAGTACCGGGATTAAAAATCCAAGGACGAATTGACATAGAGTACAAGTGTAACTATCTGCAA *F GGACAATATCTGCCTGCCCCGCTTCCAAAGTGTATTTTCCCTCCTGGATACACCGTTCGGTCCACATCATCCATGCAAG *F GTGTTACGCATCAGAATGGCGTTTATCATCGCGGAATGAGTGGGGAAATGGCTTATGAACTCCAAACAGAACGTCAAAA *F ACTACTTGCTCTGCTGGCAAAATATCGGGATCTGGAAAGAAGAAGTGAATGGTGGTCATCGGAGGAAACTGTTGTCACC *F ATGAGCAGTTATTCCTTGTATCAGAGCAACAATTTGGATATCGTCATCGACAAAACCCCACGACCAGCACTTTGCACCA *F CTTGGGGTGGTATTAACATGAAAACCTTTGATGGTCTGGTTTTCAAGGCTCCTCTATCCTGTTCACACACGCTAATTAC *F AGACAAGGTCTCGGGTACATTCGACATTATACTGAAGGCTTGTCCTTATGGCTCTGGGTATGGCTGCGCACACACCCTG *F AAAATTCTATGGCAATCGGTTCTATACACTTTTGAAAACCTGAATGGCACCATGCAGCTGACCACACCGATTAAGAAGC *F TTCCAATGCCTGTCCAGGTTATGGGCATGAAGGTTATGCCGGTGGCCCAGCATGTGCAGATCGATCTCGAGTCTGTAGG *F CCTCAAGCTGGATTGGGATCATCGGCAGTATGTGTCCGTGC! *F AGGCGGGACCGCAAATGTGGGGAAAAGTTGGAGGTCTCTGTGGAACCCTGGATGGTGATCCTA! *F ACACGGACTTGACCTCCAGAACGGGCAAAAAGTTGGCCACGGTCAAGGCCTTCGCAGATGCCTGGCGCGTCGAGGATCG *F CAGTGAATTGTGCCAGGTGGAGAATAGTGCAGAGATGGAGTTCGGTATGGATTCCTGTGAGCAATCCAAGCTACAAAAG *F GCTGTATCAGTCTGTGAGCGTCTGTTGGCCAACGAAAAGCTTGGTGACTGCATTAAACCCTTCAATTACGACGCCCTGA *F TCCGCACCTGCATGGCGGACTACTGCAATTGTGCCAATCGAGAGCACCCGGAAAGCTGCAATTGCGATGCCATTGCCAT *F GCTGGCCAAGGAGTGCGCCTTCAAGGGCATTAAACTGGAGCACGGTTGGCGCAATCTGGAAATCTGTCCCATCAGTTGT *F GGCTTTGGGCGCGTTTATCAGGCCTGTGGTCCAAATGTGGAGCCGACTTGCGATTCAGACCTAGCGCTACCAGCCTCAA *F AAGGCGCATGCAACGAAGGCTGTTTTTGTCCGGAAGGAACTGTGCAGTACAAGGAGGCGTGCATCACTCGAGAACTTTG *F CCCGTGTTCCCTTCGCGGCAAGGAATTCAAGCCAGAGTCGACGGTCAAAAAGAATTGCAACACCTGCACCTGTAAGAAC *F GGTCAGTGGCGGTGTACGGAGGATAAATGTGGCGCCCGCTGTGGGGCCGTTGGAGATCCGCATTATCAGACGTTCGATG *F GCAAACGATATGATTTTATGGGAAAATGCTCCTATCATCTTCTGAAGACCCAAAATACCTCGGTAGAGGCGGAGAATGT *F GGCTTGCTCGGGAGCCGTTTCGGAGTCAATGAATTTTGCCGCTCCCGATGATCCGTCATGCACAAAGGCGGTGACCATC *F CGATTTATCCTGCGCGATGGCACCCCATCGGTGATTAAGCTCGATCAAGGACTGACCACGATAGTTAATGACAAGCCCA *F TCGCCAAGTTACCCAAGATGCTGGGCCTGGGCGAGGTCCTC! *F ATCCGGCGGGCTAGTTCCACCTTCCTGACAGTGGAGTTCGCAGATGGCATTCGCGTCTGGTGGGACGGAGTTTCACGGG *F TGTATATCGATGCACCGCCCAGTTTGCGAGGCCAAACTCAGGGCCTTTGCGGAACCTTTAACTCCAACACACAGGATGA *F CTTTCTAACGCCTGAGGGCGATGTGGAAACTGCCGTGGAACCCTTCGCGGACAAGTGGCGGACAAAGGACACTTGTCAA *F TTTAAGGCTGAAACCCATCAAGGACCTCATCCCTGCACGTTGAACCCGGAGAAGAAAGCCCAGGCTGAGAAGTTCTGTG *F ATTGGATACTTCAGGACATCTTCCAGGACTGTCACTTTCTGGTGGAGCCAGAGCAGTTCTATGAGGATTGCCTCTACGA *F TACATGCGCCTGCAAGGATGAAATGTCCAAGTGCTTCTGTCCCATCCTTTCCGCCTATGGCACAGAATGCATGCGACAG *F GGTGTTAAGACAGGCTGGCGAATGTCTGTTAAGGAATGCGCTGTAAAATGTCCTTTGGGTCAAGTGTTTGACGAGTGCG *F GTGATGGCTGTGCCTTATCCTGTGACGATCTCCCCAGCAAAGGATCTTGCAAGCGGGAGTGCGTCGAGGGATGTCGTTG *F TCCCCATGGAGAATATGTCAACGAAGACGGCGAGTGCGTGCCGAAGAAGATGTGTCATTGCAATTTTGACGGCATGTCC *F TTCCGACCGGGCTACAAAGAGGTGCGACCAGGAGAGAAATTCCTAGATTTGTGCACCTGCTCAGACGGCGTGTGGGACT *F GCCAGGATGCCGAGCCGGGGGACAAAGATAAGTATCCGCCATCATCAGAGCTGCGCTCAAAATGTGCCAAGCAACCCTA *F CGCCGAGTTTACCAAATGTGCGCCCAAGGAACCGAAAACGTGCAAGAACATGGACAAGTATGTGGCCGACTCTTCAGAT *F TGTCTTCCCGGATGCGTTTGCATGGAGGGATACGTATATGA! *F TACTTCCCGCTTGGCCTGCGTTCTGCCAGCCAACTGCTCCTGCCACCATGCCGGAAAGAGCTA! *F CGATGACGGCGAGAAGATCAAGGAGGACTGCAATCTCTGTGAGTGCAGGGCTGGCAACTGGAAATGCTCGAAAAATGGC *F TGCGAGTCTACGTGCAGCGTTTGGGGTGATTCCCACTTCACCACCTTCGATGGCCACGACTTCGACTTCCAGGGAGCTT *F GTGACTATGTGCTTGCAAAGGGAGTTTTCGACAATGGCGATGGCTTCAGCATAACCATCCAGAATGTTTTGTGTGGCAC *F TATGGGTGTGACTTGTTCAAAGTCGCTGGAAATCGCTCTCACTGGCCATGCTGAAGAATCGCTGCTTCTGAGTGCGGAC *F TCTGCGTACAGCACTGATCCCAATAAGACGCCTATTAAAAAGCTTCGTGACAGTGTCAACTCAAAAGGTCACAATGCCT *F TCCACATCTACAAAGCTGGTGTTTTTGTGGTGGTGGAAGTGATCCCGCTGAAGTTGCAAGTGAAATGGGACGAAGGCAC *F GCGGGTTTATGTGAAGTTGGGCAACGAGTGGCGCCAGAAAGTCAGCGGCCTGTGCGGTAACTATAATGGCAATAGTCTG *F GATGATATGCAGACACCCTCGATGGGATTGGAGACGAGTCCCATGCTCTTTGGCCATGCCTGGAAATTGCAGCCTCACT *F GCTCAGCTCCAGTGGCGCCCATCGATGCCTGCAAAAAGCATCCGGAGCGCGAGACCTGGGCGCAACTGAAGTGTGGAGC *F TCTGAAGTCGGATCTGTTCAAGGAGTGCCATGCTGAAGTTCCTCTGGAACGTTTCTGGAAACGATGCATTTTTGATACG *F TGTGCCTGCGATCAGGGCGGCGATTGCGAGTGTCTCTGCACTGCTGTGGCTGCCTATGCGGATGCTTGTGCCCAGAAAG *F GCATCAATATTCGCTGGAGATCACAGCATTTCTGCCCCATGCAATGTGATCCCCACTGCTCGGACTATAAAGCCTGCAC *F TCCTGCCTGTGCTGTGGAGACATGTGATAACTTCCTCGATC! *F AGGGAATCGCGGAGCGCATGTGCAACCGTGAGAACTGCCTCGAGGGATGCCACATCAAGCCGTGCGAGGATGGATTCAT *F TTACCTAAACGACACCTACCGCGATTGTGTGCCCAAGGCCGAGTGCAAGCCAGTGTGTATGGTGAGAGATGGAAAGACA *F TTCTACGAAGGTGACATTACCTTTACGGATTCATGTGCCACCTGTCGGTGCTCCAAGCGCAAGGAGATTTGCAGCGGTG *F TAAAGTGTGATGTTCCTGTAACGACAGGACATCCGGCTCCACTCGTGGAGGGCACGACTCTACCAACGCCTCTGGCTAC *F TCAAAATCAGACCAAGTGCGTCAAGGGATGGACTCGGTGGTGCGACAAGGATCGGGATACATCGGACAAGAGTGTGCGA *F CTAAATGATGAGGAGAAGGTGCCACGCTACGATAGAATGGAAAATGTCTATGGCACATGCCTAAAGCAATATATGACCA *F AGGTGGAGTGCCGGGTGAAGGATACCCACGAGGCACCGGAGCAAATGGACGAGAATGTGGTTTGCAGTCTGGAAGAGGG *F TCTTCGGTGTATTGGAAAATGCCACGATTATGAACTGCGCGCCTTTTGCCAGTGCGATGAGGAACTAGAGCCCGAAGTG *F CCCAAGCCAACTGAAAAGCCACAACTTGGTCTGGCCTGCGATGCCGCCGTTGTGGAGTACAAGGAATTCCCAGGAGATT *F GCCATAAGTTTTTGCATTGTCAGCCCAAGGGAGTTGAGGGCGGATGGATCTACGTGGAAAAAACCTGCGGGGAGTACAT *F GATGTTTAATCCGACCATGTTAATTTGTGATCATATCGCAACCGTAACAGAGATCAAACCAAACTGTGGCTTGAAACCC *F GAACCAGAACCGGAATTTGAGCCCATCAAGCAATGTCCACCAGGCAAAATAAAGTCGGAATGTGCCAATCAGTGTGAAA *F ATACGTGTCATTACTATGGCAGCATTCTAAAGAAGAGAGGT! *F CTCTGTCAGGTTGGTGAACACTGTAAGCCTGGTTGTGTGGACGAGCTGCGACCAGATTGCCCC! *F AAACTTGGAAAGTTTTGGCGCGATGAGGACACTTGCGTCCATGCTGATGAATGTCCTTGCATGGACAAGGCGGAACACT *F ACGTTCAGCCGCACAAACCCGTCCTTGGAGAGTTTGAGGTGTGTCAGTGCATCGACAATGCGTTCACCTGTGTGCCCAA *F TAAACCAGAACCAGTTCCCAAGGATGAAGATGACGATCTGGATCTGGTATCTGTGGTTCCCATTTACCCTGTTACCCTA *F ACACCACCTCTACAATGTTCTCCAGAGCGCCTTATTCCCAAGATTGAAAACCCTGCACATTCACTGCCCGATAGCATTT *F TCAATGCCAGTTCTCAATTGGCGCCTGAACATGGCCCGAAAATGGCTCGTCTGACCAAAGAACAACCGAGGGGCAGCTG *F GTCACCTAGCATTAACGATCAGATGCAGTATCTGGAACTAAACTTTGCGAAGCCAGAGCCATTCTATGGTGTGGTTATG *F GCTGGTAGCCCGGAGTTTGATAACTACGTTACTCTATTCAAGATCCTTCACAGCCATGATGGAATCGCCTACCATTATC *F TGGTGGACGAAACGGAAAAGCCTCAAATGTTCAATGGCCCCCTAGATTCAAGGGCTCCAGTCCAGACTCTGTTTAAGAT *F CCCCATTGAAGCTAGTTCCCTTCGCATTTATCCCCTTAAATGGCATGGCTCCATCGCCATGCGTGTGGAGCTCCTGATC *F TGTGGAGATAAGGAAGAGCCTAAGCCAGTGCCCACAGTTTCAACAATTCTACCAATCACTGAGCGACCAGCTCGATTGG *F TAGATTTGGAATGCATTGATCTCATGGGCGTGGATGAGGGTAAAATGTACCAGGATCAAGTTCAATCGAGCAGTTTGTG *F GCAGCAACCCAATTTGGGAAAGAAATTACAGCTTCTGGAGCTGTTAAAGCTATCGACACCACTAGCTTGGCGCCCCTTG *F GCAAATAGTCAAAACGAGTTCATTGAGTTTGATTTCTTGGA! *F GCCACGAAATATATCTGGATTTGTCACCAAGGGTGGTCCAGATGGATGGGTAACTGGCTACAAGGTGATGTTCTCGAAG *F AAGAAACCCACTTGGAATACAGTTCTCTCAACCGACGGACAGGCACGCATCTTCGAGGCAAATCACGATGCTGAAACTG *F AGCGCAGACATCATTTTAAGAATCCCATTCTAACGCAGTACATTAAGATTGTGCCGGCTTACTGGGAGAAGAACATCAA *F TATGCGAATCGAACCACTCGGATGCTTCTTACCATATCCTGAAATACAACGTCAAGTGCCCGTTGAGGAGAACAAGCCC *F ACCAAGTGCAATATCTGCGATGGTGTGTCGACCTCCAGTTCGACTACTGGATGTCAGTGCCAGGATCAACTCTTCTGGG *F ATGGCAATACCTGTGTACAACACAATCTCTGTCCTTGCATAGAGAACTATGTGAGCTATCCGATAGGTAGCAAATTTGA *F GAACTCAGCTTGCGAGGATTGCGTCTGTGTGCTTGGTGGCCATAAGAATTGCAAACCCAAGAAGTGCCCGCCATGTCTG *F GGCGGTAAATTGCGTCCAGTGATTACCAGCGACTGTTTCTGTAAGTGCGAACCTTGTCCCAAACACCAAAGATTGTGCC *F CGTCCAGTGGAGATTGTATACCCGAAATTCTGTGGTGCAATGGTGTTCAGGATTGTGCTGATGACGAGGATGCCAGTTG *F CAGTGATTCATTCACTGTTGAACCTGACGTCAGCCGAGAAAAGAATGAGACTGAAGTCATTACATGCCCCGTCCCCGTT *F TGTCCGCCTCAAATGAAAATAAGAATTACGGAGAAAAAGTCTAGAAAGATGTCAAAGATGTTCACCTTCTCGAAGCAGG *F TGTCAATAGTGGACGATGGAACGACCATCACAAAGACCAAGTTCATCTCGTCCAAGGAACAAATTCTGGCTATGCCGAA *F CCGGGAATTGGACTTTCAACTGGAAGAGCAGTGCGATGAGT! *F TCACTTGTGTGCCCATTCCCAGCAAGCAAGTGGACAAAAACGAGACTGTCACCTGCACGGAAC! *F CAAAGTGTCCTGAGAAATACGATGTGGAATTGGACATGAGTGCGTCGAAAGTGGGTGACTGTCTGAGATACAGCTGCGT *F CCTTCGGCCCAACAAAGACGATGTGTGTGAAATCAGTGGCAAGAGTTTTACCACCTTTGATGGAACTGTTTTCAAATAC *F GGACCTTGCAGCCACATCCTGGCCAGAGATATTCATAGCAGTAGTTGGTCAATATCGGTCCATCAGCAGTGTAGCGATG *F AGACACGTAAAGTCTGTCACAAGGTGATCACCATTCAAGACACCGAGGCTGGCAATGAGTTGATCCTTTTGCCTCATCT *F GAAGCTCAAGTTTAATGGCTATGAGTTCACAGTGCAGCAATTGATAAATTCGCCCATTTGCAAGGCTTCATTCGTTGTT *F TCCCAGCCGGGCAAGACTCTTCTGGCCGTGTCCACCAAATATGGCTTCTGGGTGCAGCTCGATGACATTGGAATCGTCA *F AAGTGGGCATATCATCCAAGTTTATTCGCACAGTCGATGGATTGTGTGGCTACTACAATGGAAACCAAAAGGATGACAA *F GAGATCACCAGATGGTCAGATTATTCCAAATACCGAAAAGTTCGGCGACAGTTGGTATGATAAGCGGATACCCAAGGAC *F CAATGTGGAGATCTTAAGTGCCCCAGGGAGATGCAGGCAAAGGCCTTGCAGCTGTGTAACATCATTCATCATCCAACCT *F TTGCTCGTTGCCACAAGGCAGTTAACTACAAGCAATTCCTTAACAATTACTGCCTCGAAGCTGCCTGCAATTGCATGAT *F GGCCAACAATGGAGATCCTGCCGCCTGCAAGTGTAACATCTTGGAGAGCTTTGTGAAAAAGTGTCTTAGTGTAAATCCA *F CTAGTTCAATTGACTACATGGAGAGCTGTGGCCCAGTGCGAGATTAATTGTCCCTCGCCATTAGTGCACACCGACTGTT *F ACAAGAGACGTTGTGAGCCATCTTGCGATAATGTTCATGGA! *F GACGACTGCCCAGTACTTCCGGATGCCTGCTTCCCGGGATGTTATTGTCCAGAGGGAACTGTCCGTAAGGGTCCCAACT *F GCGTGCCCATATCCGAGTGTAAAGACTGTGTGTGCAATTCATTGGGCGCCTCCAAGTACATGACCTATGACCGGAAGAG *F TTTTAGCTTTAATGGAAACTGCACCTATCTGCTGTCACGCGATGTCGTCCTCCCTGGTGTCCACACATTCCAGGTATAC *F GTTAGCATGGATGACTGTAAGAAACTGGGACAACCAACTCCAGTGGAAGGCGGCAGCTGCGCTAAATCACTGCACATTC *F TCAACGGGGACCATGTGATTCATGTTCAGCGCGTTCCACAGAAACCCAAATCCCTGCAGGTCCTGGTCGACGGATTCGA *F GGTTAAGAAAATACCATACAAAGATAGTTGGATTAGCCTTCGGCAAGTGGTTGGCAAGGAACTGGTGCTCTCCCTTCCG *F GAATCCCATGTGGAATTGACTGCCTCCTTTGAAGACCTGATATTCTCCTTGGGCGTACCAAGTATCAAATATGGAAGCA *F AAATGGAGGGACTTTGCGGCGACTGTAATGGCAATGCAGGCAATGATCTCCAGCCGAATCCGGCTAAAAAAAAAGCAGG *F AGTGGATGTCATCCAAAGCTGGCAGGCGGATGAGCCTAAGCTGGGATTGGTTGAAGAGTGTCTTAGTGAGGATGTGCCC *F AAGGAGCACTGCATACCCCTACCGCCAGAGAAGGATCCCTGTTTGCAGTTCTACAATGCTGAATTATTTGGCAAGTGTC *F CTTTGGCTGTGGATCCCATTGCCTATGTCTCCGCCTGTCAGCAGGACATCTGCAAGCCCGGAAACACCCAGCAAGGAGT *F CTGCGTGGCTCTGGCCGCTTATGCCAAGGAGTGTAACCAGCACGGCATATGCACCAATTGGAGAAGACCGCAACTGTGT *F CCCTACGAATGTCCCAGCGATATGGTTTACGAGCCATGCGG! *F CTGTGCTAAGAATTGCGATACCATCAAGGCATTATCCGAATTTGATGCAGTGAGCCTTAAAAA! *F CGAGGCTGTAGTTCATACCGTTAAGACTGATGAAATGTGTTTGAATTCGGAGCGATTCGAGGGCTGCTTCTGTCCTCCT *F GGAAAGGTAATGGATGGTGGACAGTGTGTGCCCGAAATAGCCTGCACAAAGTGCGATGATGGACTCCACTTGCCTGACG *F AGAAATGGAAGAAGGATAAGTGCACCGAATGTCAGTGTGATTCTAAAGGAAAGACCACGTGTGTGGAAAAGAAGTGCCA *F GGTGGAGGAAAATATCTGCGCCGAAGGCTATAGACCTGAGACCATAGTCAGCGTGGATGAATGCTGTCCTAGATATCGA *F TGTGTCCCGGAGACGAAGGACCCATCCAAACTGTGCCTTGCTCCTCTAGTGCCCATTTGCGGTCCTGGACAATTTAAAA *F AGGAAAAGAAGGATGTCAATGGTTGTTCGCAGTACATATGCGAGTGCATACCGAAGGACCAGTGCGAAATAATTGAGCT *F GCGGGAGCTGCTTCCAGGCGAGATCATTGTGAATGTAGAAGAAGGCTGCTGCCCCACTCAGAAGATTGAGTGTAAGCCG *F GAGACTTGCCCCAAGGCGCCTGTTAACTGTCAAGAACGATTCTACGAAGTAAAGACCATCAAGGAGCCAGGAATGTGCT *F GCTCCAAGCACTCATGTGTACCTCCCAAGGATCTGTGCATTGTTCAGTATGAGCTCGACGAAGCCACAAAGTTCACCAA *F GACAGTGGGTGATAAGTGGACCCATGCCAAGGAGGTTTGCAAGCAGGAGACATGTTCCTATGGTCCGGATGGCAATGCC *F CAGGTTGTCAGCACTTTGGAACAGTGTCTCACTGACTGCGCCCCAGGATTCAGCTACCAGAACTTGGACAAAACCAAAT *F GCTGTGGCAAGTGTGTCCAGACTTCGTGCATCTTTGAGCAAAAACTATACGAAGTCAACGCACTTTGGAAGTCGACGGA *F CAATTGCACTACCTACAGTTGCCTGAAGAAGGATGGCCAAT! *F TTTTGGTTACCACCTCGAGAGAAGTATGTCCGGATGTGGGTAGTTGCCTATCCCACCTGTTGTACCAAGATGGCTGCTG *F CAAACGATGCAAGTCGGAACCATTGGTGGAGGACAAATCATCTTGTTTACCCGTTTCCTTGGCCGAGTCGCGTACGAAG *F GAGATTCTCAAGTTCCCTGTGCAAGGACATGGAACATGTGTGAATGCCGATCCCATTCAGGGCTTCACCGACTGCGAGG *F GTGCCTGCTCCTCTGGTTCCAAGTACAATACACTCACGGACATGCACGAGAAGTTCTGTACTTGCTGCAGCATAAAATC *F CTATCATCCGATTTCCGTGAAAATGATTTGCGATGATGGTCACACATTTACACAGAAGCACGAGGTGCCCTCCAACTGC *F GGATGCTCGCCGTGCTCAGAGTTTTCGGACTCTGCAATCGATGTGCGAATGCAGCAGGATGCGCAGTCTCCACTACTCC *F AGTTACTCGGCAACCATAGACATTAATAATTTATTGGTTGTCTGAGAAAGTCATTTCGTTAGTTTTAAGAATGTCGAGC *F ATAAAAAATAAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA *F Protein sequence: *F MANKVIFVALWLLFISSVLTENGDIIITDDDDEDINPLLQAAPEVVDKENERNERHISFNILKAPAVSARYTYGGSEGG *F RSGFGGGYGVGGGGGGGYGGIKTSHAAGGLLTKTLGFLGMGGQGSQGNFYGGGGHFLAAFGKCSALQLPSNVQSECHNG *F RCKVFCPTGYSFAQDVSVLEMFCSNDGWIIGNSVFAEVPPCQAQCTPPCQNNGICISAGVCQCPENYYGPLCQQKKSIC *F ASFPKAPKNSKVSCKNNMCHAECMRGFQFPDGSGITNIECRNGQWVHTKTGLSKTPDCAPTCAPACQNGGQCISFNVCQ *F CSKMFRGDHCQYNIDRCNVTNTNFNGNYKCAYEMDDARCTFSCPQVPGLKIQGRIDIEYKCNYLQGQYLPAPLPKCIFP *F PGYTVRSTSSMQGVTHQNGVYHRGMSGEMAYELQTERQKLLALLAKYRDLERRSEWWSSEETVVTMSSYSLYQSNNLDI *F VIDKTPRPALCTTWGGINMKTFDGLVFKAPLSCSHTLITDKVSGTFDIILKACPYGSGYGCAHTLKILWQSVLYTFENL *F NGTMQLTTPIKKLPMPVQVMGMKVMPVAQHVQIDLESVGLKLDWDHRQYVSVQAGPQMWGKVGGLCGTLDGDPNTDLTS *F RTGKKLATVKAFADAWRVEDRSELCQVENSAEMEFGMDSCEQSKLQKAVSVCERLLANEKLGDCIKPFNYDALIRTCMA *F DYCNCANREHPESCNCDAIAMLAKECAFKGIKLEHGWRNLEICPISCGFGRVYQACGPNVEPTCDSDLALPASKGACNE *F GCFCPEGTVQYKEACITRELCPCSLRGKEFKPESTVKKNCNTCTCKNGQWRCTEDKCGARCGAVGDPHYQTFDGKRYDF *F MGKCSYHLLKTQNTSVEAENVACSGAVSESMNFAAPDDPSCTKAVTIRFILRDGTPSVIKLDQGLTTIVNDKPIAKLPK *F MLGLGEVLIRRASSTFLTVEFADGIRVWWDGVSRVYIDAPP! *F SLRGQTQGLCGTFNSNTQDDFLTPEGDVETAVEPFADKWRTKDTCQFKAETHQGPHPCTLNPEKKAQAEKFCDWILQDI *F FQDCHFLVEPEQFYEDCLYDTCACKDEMSKCFCPILSAYGTECMRQGVKTGWRMSVKECAVKCPLGQVFDECGDGCALS *F CDDLPSKGSCKRECVEGCRCPHGEYVNEDGECVPKKMCHCNFDGMSFRPGYKEVRPGEKFLDLCTCSDGVWDCQDAEPG *F DKDKYPPSSELRSKCAKQPYAEFTKCAPKEPKTCKNMDKYVADSSDCLPGCVCMEGYVYDTSRLACVLPANCSCHHAGK *F SYDDGEKIKEDCNLCECRAGNWKCSKNGCESTCSVWGDSHFTTFDGHDFDFQGACDYVLAKGVFDNGDGFSITIQNVLC *F GTMGVTCSKSLEIALTGHAEESLLLSADSAYSTDPNKTPIKKLRDSVNSKGHNAFHIYKAGVFVVVEVIPLKLQVKWDE *F GTRVYVKLGNEWRQKVSGLCGNYNGNSLDDMQTPSMGLETSPMLFGHAWKLQPHCSAPVAPIDACKKHPERETWAQLKC *F GALKSDLFKECHAEVPLERFWKRCIFDTCACDQGGDCECLCTAVAAYADACAQKGINIRWRSQHFCPMQCDPHCSDYKA *F CTPACAVETCDNFLDQGIAERMCNRENCLEGCHIKPCEDGFIYLNDTYRDCVPKAECKPVCMVRDGKTFYEGDITFTDS *F CATCRCSKRKEICSGVKCDVPVTTGHPAPLVEGTTLPTPLATQNQTKCVKGWTRWCDKDRDTSDKSVRLNDEEKVPRYD *F RMENVYGTCLKQYMTKVECRVKDTHEAPEQMDENVVCSLEEGLRCIGKCHDYELRAFCQCDEELEPEVPKPTEKPQLGL *F ACDAAVVEYKEFPGDCHKFLHCQPKGVEGGWIYVEKTCGEYMMFNPTMLICDHIATVTEIKPNCGLKPEPEPEFEPIKQ *F CPPGKIKSECANQCENTCHYYGSILKKRGLCQVGEHCKPGC! *F VDELRPDCPKLGKFWRDEDTCVHADECPCMDKAEHYVQPHKPVLGEFEVCQCIDNAFTCVPNK! *F PEPVPKDEDDDLDLVSVVPIYPVTLTPPLQCSPERLIPKIENPAHSLPDSIFNASSQLAPEHGPKMARLTKEQPRGSWS *F PSINDQMQYLELNFAKPEPFYGVVMAGSPEFDNYVTLFKILHSHDGIAYHYLVDETEKPQMFNGPLDSRAPVQTLFKIP *F IEASSLRIYPLKWHGSIAMRVELLICGDKEEPKPVPTVSTILPITERPARLVDLECIDLMGVDEGKMYQDQVQSSSLWQ *F QPNLGKKLQLLELLKLSTPLAWRPLANSQNEFIEFDFLEPRNISGFVTKGGPDGWVTGYKVMFSKKKPTWNTVLSTDGQ *F ARIFEANHDAETERRHHFKNPILTQYIKIVPAYWEKNINMRIEPLGCFLPYPEIQRQVPVEENKPTKCNICDGVSTSSS *F TTGCQCQDQLFWDGNTCVQHNLCPCIENYVSYPIGSKFENSACEDCVCVLGGHKNCKPKKCPPCLGGKLRPVITSDCFC *F KCEPCPKHQRLCPSSGDCIPEILWCNGVQDCADDEDASCSDSFTVEPDVSREKNETEVITCPVPVCPPQMKIRITEKKS *F RKMSKMFTFSKQVSIVDDGTTITKTKFISSKEQILAMPNRELDFQLEEQCDEFTCVPIPSKQVDKNETVTCTEPKCPEK *F YDVELDMSASKVGDCLRYSCVLRPNKDDVCEISGKSFTTFDGTVFKYGPCSHILARDIHSSSWSISVHQQCSDETRKVC *F HKVITIQDTEAGNELILLPHLKLKFNGYEFTVQQLINSPICKASFVVSQPGKTLLAVSTKYGFWVQLDDIGIVKVGISS *F KFIRTVDGLCGYYNGNQKDDKRSPDGQIIPNTEKFGDSWYDKRIPKDQCGDLKCPREMQAKALQLCNIIHHPTFARCHK *F AVNYKQFLNNYCLEAACNCMMANNGDPAACKCNILESFVKKCLSVNPLVQLTTWRAVAQCEINCPSPLVHTDCYKRRCE *F PSCDNVHGDDCPVLPDACFPGCYCPEGTVRKGPNCVPISEC! *F KDCVCNSLGASKYMTYDRKSFSFNGNCTYLLSRDVVLPGVHTFQVYVSMDDCKKLGQPTPVEGGSCAKSLHILNGDHVI *F HVQRVPQKPKSLQVLVDGFEVKKIPYKDSWISLRQVVGKELVLSLPESHVELTASFEDLIFSLGVPSIKYGSKMEGLCG *F DCNGNAGNDLQPNPAKKKAGVDVIQSWQADEPKLGLVEECLSEDVPKEHCIPLPPEKDPCLQFYNAELFGKCPLAVDPI *F AYVSACQQDICKPGNTQQGVCVALAAYAKECNQHGICTNWRRPQLCPYECPSDMVYEPCGCAKNCDTIKALSEFDAVSL *F KNEAVVHTVKTDEMCLNSERFEGCFCPPGKVMDGGQCVPEIACTKCDDGLHLPDEKWKKDKCTECQCDSKGKTTCVEKK *F CQVEENICAEGYRPETIVSVDECCPRYRCVPETKDPSKLCLAPLVPICGPGQFKKEKKDVNGCSQYICECIPKDQCEII *F ELRELLPGEIIVNVEEGCCPTQKIECKPETCPKAPVNCQERFYEVKTIKEPGMCCSKHSCVPPKDLCIVQYELDEATKF *F TKTVGDKWTHAKEVCKQETCSYGPDGNAQVVSTLEQCLTDCAPGFSYQNLDKTKCCGKCVQTSCIFEQKLYEVNALWKS *F TDNCTTYSCLKKDGQFLVTTSREVCPDVGSCLSHLLYQDGCCKRCKSEPLVEDKSSCLPVSLAESRTKEILKFPVQGHG *F TCVNADPIQGFTDCEGACSSGSKYNTLTDMHEKFCTCCSIKSYHPISVKMICDDGHTFTQKHEVPSNCGCSPCSEFSDS *F AIDVRMQQDAQSPLLQLLGNHRH *F Comments: Dear Flybase officers *F We have recently completed a cDNA sequence of hemolectin (hml) from Drosophila *F larval staged cDNA library (deposited in Genbank database: AB035891). We also *F found the same hml cDNA sequence analyzed by Flybase informatics as a number *F of Gadfly: CG7002. Of course, as the source is quite different (larvae v.s. y, *F cn bw sp strain), we could observe many nucleotide changes probably due to the *F polymorphism. However, there are two big differences between AB035891 and *F CG7002 that we can not overlook. First, according to our results of cDNA *F cloning, mRNA length (from ATG to TAA) is 11532bp instead of 10935pb. Second, *F based on our genomic sequence analysis referring to Genbank sequence *F (AC015143), we found that hml has 26 exons instead of 23 exons. Although it is *F not still accepted, we will publish a paper about detail strategy of hml cDNA *F cloning and analysis of the domain structure. So, after your confirmation, we *F request you these corrections described above on BDGP database. *F .. *F Sincerely yours, *F Yasuo Kitagawa *F Browser: Mozilla/4.7 ja (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135274 *a Brill *b J. *t 2001.3.29 *T personal communication to FlyBase *u FlyBase error report for CG7004 on Thu Mar 29 13:08:29 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Mar 29 22:08:31 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 29 Mar 2001 22:08:31 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 29 Mar 2001 13:08:29 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: jbrill@sickkids.ca *F Subject: FlyBase error report for CG7004 on Thu Mar 29 13:08:29 2001 *F Content-Length: 701 *F Error report from Julie Brill (jbrill@sickkids.ca) *F Gene or accession: CG7004 *F Release: 1 *F Gene annotation error *F Gene CG7004 corresponds to AF242375 (FBgn0004373) *F Comments: My paper describing the cloning of fwd came out in Development in *F Sept. 2000: *F Brill, Hime, Scharer-Schuksz and Fuller. Development (2000) Sep;127(1):3855-64. *F A phospholipid kinase regulates actin organization and intercellular bridge *F formation during germline cytokinesis. *F The GenBank Accession number corresponds to a head library cDNA, GH11214. Note *F that this cDNA includes an exon that was missed by the gene prediction programs. *F Browser: Mozilla/4.74 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135275 *a Eaton *b S. *t 2001.3.30 *T personal communication to FlyBase *u FlyBase error report for CG12342 on Fri Mar 30 01:36:53 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Mar 30 10:37:04 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 30 Mar 2001 10:37:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 30 Mar 2001 01:36:54 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: eaton@mpi-cbg.de *F Subject: FlyBase error report for CG12342 on Fri Mar 30 01:36:53 2001 *F Content-Length: 4706 *F Error report from suzanne eaton (eaton@mpi-cbg.de) *F Gene or accession: CG12342 *F Release: 1 *F Gene annotation error *F Gene CG12342 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG12342 *F CCCCCGGGCTGCAGGATTCGGCACGAGGCCAAATTAACTCTGGGCGTTCCATCAATCCATCAATGGCCAAATACTGCAA *F AGTCGGGTACTGAACAATAGTCGAAAGTAAACATGTGTAAATACGTGAGAGTGGCGAATTGCAATGCGTAAATTGCGGT *F CCAAAGACGGGATTTAAAATTAGTGAAAAACAATGCAGCATGGATCCTCCCGCATTCCGAAACAGGAAGCTCTCCCGAA *F TGCGATATCCGGGCCCGACTCCAGCAATTGGACTTCGCTGCACGAGGCGGTAGCCGCCGGAGATGATCGCCGGGTGGAG *F GGTCTCCTGTTCAGCAATGCTGATCGACTGGCCAGGGAATCCGTGCAGGGGAATACCCCGCTCCATGAGGCCGCCAGTC *F GGGGGTTCAGTCGCTGCGTGAAATTGATCTGCACGCCCCCCACGCCACCCGCTTCCTCGGGGGTGGGAAAAGCAGCAAA *F GGGACAGAAGGACAAAGACAAGGATAAGAACAAGGGCAGAGTGAAGGTGGCCCACCAGACCATCGAAGCCCTACACAAT *F AGCGCCCTGGGTATTGCCAACAACGAAGGGTTGTCCGCCCTGCACTTGGCCGCTCAAAATGGTCACAATCAGAGCTCTA *F GGGAGCTACTGCTGGCCGGCGCCGATCCCGATGTCCGGAATAATTATGGAGATACTCCGCTGCACACCGCCTGCAGATA *F TGGACATGCAGGAGTCTCCCGGATCCTGCTCTCCGCCCTTTGCGATCCGAATAAAACAAATCTGAACGGCGACACCGCT *F TTGCACATCACCTGTGCCATGGGTCGCAGGAAGCTGACAAGGATTCTCCTAGAGGCAGATGCTCGGCTGGGCATTAAAA *F ACGCACAAGGCGACTGCCCCATGCACATAGCGATCAGGAAGAACTACCGTGAAATCATCGAGATCCTGAACACGCCGAA *F GAAGATACGGAATCGCAAGGAGAAACCCAAGGAGGGCGGCA! *F GCAGATCGGACAAGGACAGAGATCGTGAAAGGGATGTGGTGGACAAGGGTATCAACTGGTCACCCTATGGCTGTCACTA *F CTTTCCCGATCCGCGTGCCTTTCCTTCACCAAAACTGGAGACACTGCCCAAGGAGCCACTTAAGTCGGGGGAGCAATAC *F TTCCTGGATTTGGCTGGTCATATTCACAAGGGTCCGGTCAGCGTGGGCAACACATGCTATTGCGGTCCCTTCTTCCGTC *F ACATTGAAAACAAACTAAATTGCAATCGAAAGAGTTTGAAGAAATACGTTCACAAAACCAAGGAACGTCTAGGTCACAA *F AGTTCAGGCATTGGCAATCAAGACGAACGATCAGATTGAACAGCTCACAAGAACGATGATCGAGGACAGATTGCGATGC *F GAGAGCAAGAGGCAGCACCTCAGTGAGTTCCTGCGACGAGGTGAACCTATGCGGTCCACCTTCGATCAGCAGAACAAGA *F GCTCAAGGATCGAAAGAACAATGTCCAGGTGCCGCAGTCTAGAGCTCTTGGAAAACAACCACGAAGGTGGTAGGCTCAC *F CAACTCACGGAGTGTAGATGTCCTGGAAGATCAGGCAGTGGAGGCTATAGTACACCGATCAGCGGAGGTCCAGGTGGAT *F AGCGACAGCGATGACGATTCCAATGAAGCTCGGGATGAAGAAGAGGCAGAAGTGGGTGACAAGGACCAGGTGCAAGAAG *F AGGCGGAACCAGAGGAGCACTCGAAGCTAGATGAACTTAAGCTAGATTTCCTAAAAGTCTCCGAAAGACTGGGAGTGCT *F TTTAGAGAAAACCACCCTGATCATGGAAAGGGACAGCGAACAGGAGAGCAAGCGCCAGCTTTCTTCCTTATCGCCACCT *F TATAATCCCCATCCAAGGGCGGAGTCTGCCCAACTTCGAGAAGATAAAGAGAGTGTTAATAGTCCCAATTTCGACGAGT *F ACGCCAACGTGATACGACGCTATCCGAACTCCAGTGAGACC! *F TCAAATCCCTCCCAGAACTCCAACAGCTGGGACTGGGAGGCATCACCCACGGGCAGCAATCCC! *F CACCCGGATTACAATAAGTACTACCAGCGCATTGGGAGAGGAGAGAACATGCTGAACTCGGTGATCAAGGCGCTTCGAA *F AAGATGCCTCCTTCGCGGATCTGGGCAAAGAGGAGACGGCTGTCAATGAAACTGCCGAGGGAATGGGAGGCGACAAGCT *F GCTCTACAAGGAGCAAGCGAGCGAGGCGGCAGGTATCTCCAATCTGATGAAACGGCAGCCTCTTTGCTTGGAAGACAAC *F TATCCCGTCATGTCGAATTTATTTTACTCCAATCCCATCATGGAGTACGCCGAGGAAACAGAAGTGCGTCAAAATGGCA *F ATGAAAGTATGAGTAAGGTGGAGATTCGGAACAGTGAAATCAAGTGCCTAAAGAAGCCGAACGCTGGTCAGGTGAGGGA *F TATGGTGGCCCAACTGCAAAACACCATTGACTCCAATCGACAGGACTCCCAGACGGCACATGTGATCGCCGCAAGATCA *F CACTCCCGCTTGAACAATAACTTCCATCACTTGGAGGGCACCTCTTCGAACCCTTCGCCCACGTATAGTATTCCCCACC *F GGAGTAGCGCCCAGTTTGGGCTGCCCGAGAGACACATACCCAAAGATGCTTACTTCCATGAACTGCCCCACCGTCCACC *F GATTCCGCAAACTCCGCAGAGAAGGCTGCACTCCGGCTACGTACCAAATGCGAGTCTCTATCGCAGTGAAGATCTTTAC *F GTGGGAAGGATTCCACATCCAAATGCTCCACCAAGACAAGTGGACTACGTGGATGCGGTAGCCTATAGGCCTCTGCAGT *F CCCCCACCTTTCAGGCCATTGTGCCGCCCCATGTTAATCAAAGGCCAAGGAACTGGCCTCATCCCAATCTTCCATCCAA *F TGAAATCGATCTGGACGAGCTGTCTGCCGTTGGCCTCTACAACAATGTCTCGAGTCTAGTTTGAGTTCGGTAATCCCAC *F AGCTGTTCCCATTGTACATAGCTAATCCCTACATATTTATC! *F TTAATTGTAATCAAGTTTTAAACTACAAAGAAAAGTTAACTTATTTTCAACTGATGCATCTAATCTGGAGGGTCTAAAA *F TATATAAATATAATACAAAGATATAAAAAAAAAAAAAAAAAAACTCGAGGGGGGGCCC *F Protein sequence: *F >CG12342 protein *F MQHGSSRIPKQEALPNAISGPDSSNWTSLHEAVAAGDDRRVEGLLFSNADRLARESVQGNTPLHEAASRGFSRCVKLIC *F TPPTPPASSGVGKAAKGQKDKDKDKNKGRVKVAHQTIEALHNSALGIANNEGLSALHLAAQNGHNQSSRELLLAGADPD *F VRNNYGDTPLHTACRYGHAGVSRILLSALCDPNKTNLNGDTALHITCAMGRRKLTRILLEADARLGIKNAQGDCPMHIA *F IRKNYREIIEILNTPKKIRNRKEKPKEGGSRSDKDRDRERDVVDKGINWSPYGCHYFPDPRAFPSPKLETLPKEPLKSG *F EQYFLDLAGHIHKGPVSVGNTCYCGPFFRHIENKLNCNRKSLKKYVHKTKERLGHKVQALAIKTNDQIEQLTRTMIEDR *F LRCESKRQHLSEFLRRGEPMRSTFDQQNKSSRIERTMSRCRSLELLENNHEGGRLTNSRSVDVLEDQAVEAIVHRSAEV *F QVDSDSDDDSNEARDEEEAEVGDKDQVQEEAEPEEHSKLDELKLDFLKVSERLGVLLEKTTLIMERDSEQESKRQLSSL *F SPPYNPHPRAESAQLREDKESVNSPNFDEYANVIRRYPNSSETSNPSQNSNSWDWEASPTGSNPHPDYNKYYQRIGRGE *F NMLNSVIKALRKDASFADLGKEETAVNETAEGMGGDKLLYKEQASEAAGISNLMKRQPLCLEDNYPVMSNLFYSNPIME *F YAEETEVRQNGNESMSKVEIRNSEIKCLKKPNAGQVRDMVAQLQNTIDSNRQDSQTAHVIAARSHSRLNNNFHHLEGTS *F SNPSPTYSIPHRSSAQFGLPERHIPKDAYFHELPHRPPIPQTPQRRLHSGYVPNASLYRSEDLYVGRIPHPNAPPRQVD *F YVDAVAYRPLQSPTFQAIVPPHVNQRPRNWPHPNLPSNEIDLDELSAVGLYNNVSSLV *F Comments: The difference between the protein predicted from the genomic *F sequence and the protein encoded by the cDNA is restricted to the first 22 *F amino acids that may result from an error in the prediction of a splice junction # *U FBrf0135276 *a Page *b S. *t 2001.4.4 *T personal communication to FlyBase *u FlyBase error report for CG12218 on Tue Apr 3 17:29:46 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 04 01:29:50 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 4 Apr 2001 01:29:50 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 3 Apr 2001 17:29:46 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: slpage@ucdavis.edu *F Subject: FlyBase error report for CG12218 on Tue Apr 3 17:29:46 2001 *F Content-Length: 299 *F Error report from Scott Page (slpage@ucdavis.edu) *F Gene or accession: CG12218 *F Release: 1 *F Gene annotation error *F Gene CG12218 corresponds to FBgn0026206 *F Comments: CG12218 corresponds to mei-P26 (FBgn0026206) *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135277 *a Richardson *b H. *t 2001.4.10 *T personal communication to FlyBase *u FlyBase error report for CG12397 on Mon Apr 9 22:33:12 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 10 06:33:30 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 10 Apr 2001 06:33:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 9 Apr 2001 22:33:12 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: h.richardson@pmci.unimelb.edu.au *F Subject: FlyBase error report for CG12397 on Mon Apr 9 22:33:12 2001 *F Content-Length: 581 *F Error report from Helena Richardson (h.richardson@pmci.unimelb.edu.au) *F Gene or accession: CG12397 *F Release: 1 *F cDNA or EST error *F Comments: I believe that this sequence represents 2 genes \- the Debcl (bcl2 *F homolog = AF178430) 5' exons have been fused to the 3' zn finger containing *F exons. The C terminus of the Debcl gene is missing and 5' exons have been *F fused to the exon of the 3' Zn finger containing gene. The zn finger gene *F exons should be listed as a separate gene. *F Browser: Mozilla/4.0 (compatible; MSIE 5.5; Windows 98) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135278 *a Millburn *b G. *t 2001.4.12 *T personal communication to FlyBase *u FlyBase error report for CG13543 on Thu Apr 12 08:22:39 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 12 16:22:37 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 12 Apr 2001 16:22:37 \+0100 *F Date: Thu, 12 Apr 2001 08:22:39 \-0700 (PDT) *F From: FlyBase-error@hedgehog.lbl.gov *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG13543 on Thu Apr 12 08:22:39 2001 *F Content-Length: 745 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG13543 *F Release: 1 *F Gene annotation error *F Gene CG13543 should be split. *F Comments: Clyne et al., 2000, Science 287(5459): 1830--1834 (FBrf0126794) *F describe 2 *F gustatory receptor genes, GR59D.1 (FBgn0041236) and GR59D.2 (FBgn0041235), *F whose sequences match the N-terminal and C-terminal half of CG13543 *F respectively. *F This suggests that CG13543 may need splitting in to 2 separate genes. *F Sequences of GR59D.1 and GR59D.2 (worked out from coordinates given in *F References and Notes of FBrf0126794) and alignments to CG13543 follow *F in e-mail to flybase-updates, *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From gm119@gen.cam.ac.uk Thu Apr 12 16:27:25 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 12 Apr 2001 16:27:25 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: CG13543 alignments *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 12 Apr 2001 16:27:21 \+0100 *F Content-Length: 12728 *F sequence of GR59D.1: *F >GR59D.1,AC006245,68825-70050 *F atggctgacctgctcaaattgtgtttgagaatcgcatatgcctacggacggttgaccggcgtaatcaactttaagattg *F atttgaaaacgggtcaagcgctagttaccagaggagctacgcttatttcagtgagcacacacttgctgatctttgccct *F actcctctaccaaacaatgcgaaaaagtgtggtgaacgtcatgtggaagtatgccaattcccttcacgaatacgtgttc *F ctggtcatagccggctttcgcgtagtctgtgtctttctggagctggtcagtcgatggtcgcagcgtcgcacctttgtga *F ggctcttcaactcattccggagactgtatcagagaaatccggatataatccagtactgccgaaggagcatcgttagcaa *F attcttttgcgtcacaatgacagagacactgcacatcatagtcaccttggccatgatgaggaatcggctgagcattgct *F ctggctctgcgcatttgggccgtattgagtctgacggccataataaatgtaatcatcacgcagtactatgtggccaccg *F cgtgtgtgcgaggacgatatgcgctcctgaacaaggatcttcaggcgattgtgaccgaatcccagtcgctggttcccaa *F cggaggtggcgtctttgtgaccaagtgttgctacctagcggatcgcttggagcgaatagccaagtcccagtcggaccta *F caggagctcgtcgaaaacttgtccacggcatacgaaggagaagtggtctgcctggtcatcacatactatctgaatatgc *F tgggcacctcgtatctgctgttcagcattagcaagtatggcaattttgggaataacctgctcgtgatcatcactctttg *F tggcattgtctacttcgtattttacgtcgtcgattgctggatcaacgcgtttaatgtgttttaccttttggatgcccat *F gataagatggttaagttgctgaataagcgaactttgtttcagc! *F caggtctggatcatcgattggaaatggttgtaagagcttcaaactctctttaatggtcgaagattttaattgaaccttc *F ttgcagtttgaaaactttgctctgaacttggtgcggaatccattgaagctccatatgtacggccttttcgagtttggtc *F gaggaacatcctttgccgtgtttaactccctgttaacacactcccttctcctcattcaatacgacgtgcaaaacttc *F \# *F sequence of GR59D.2: *F >GR59D.2,AC006245,70261-71505 *F atggttgacttggtgaagacgattttgctcattgcctactggtatggccttgccgtgggagtgtccaacttcgaggtgg *F actggctaactggagaagccattgccacccgaaggactacgatctatgcagcagtgcataatgccagccttatcactct *F gctgattcttttcaatcttggcaataactcactgaaatccgagttcataagtgctcgatatctgcatgagtacttcttt *F atgctcatgactgcggttcgaatctcggcagttctgctctcactgataaccagatggtatcagcgttccagattcattc *F gaatttggaatcagatactagccctagttcgcgatagacctcaagtggttcgtgggcgctggtatcgtcgcagcattat *F ccttaaatttgtgttctgtgtcctgtcagattctctgcacaccatatcggatgtgagtgcgcaacgaaagcggatcact *F gctgacctgattgtcaaactgagcttactggccacactgaccaccatttttaacatgatcgtgtgccagtactacttgg *F ccatggtgcaggtgattgggctctacaagattctgctccaagatctgcgatgcttggtgcgccaagctgaatgcatctg *F ctccattcgcaatcggcgaggtggagtttactccattcagtgctgctcgttggcagatcagctggatctaattgccgaa *F aggcattactttctgaaggacagacttgatgagatgtcggaccttttccagatacagagcctaagcatgagcctggtgt *F actttttctccaccatgggctccatctactttagcgtctgttcgatcctgtacagctccacaggattcggctctacata *F ctggggtcttctgctgattgtactatccacggcttccttctacatggacaattggttgtccgttaacattgggtttcat *F attcgagatcagcaggacgaactattccgagtgctggcggatc! *F gaactctgttctatcgggaattggacaaccgactggaggcagccgtaagttcagctttcgattttccccttctacgact *F tttccttaatctgtttttattttttcagtttgagaacttccaactgcaactggccagtaaccggcatgaattctacgtt *F atgggtctctttaaaatggaacgtggtcgtctaatcgctatgctaagctcagtgatcactcatactatggttcttgttc *F agtgggaaattcaaaac *F \# *F alignments to CG13543: *F 1. GR59D.1 *F \---------- *F Query= GR59D.1,AC006245,68825-70050, 1226 bases, A9493664 checksum. *F (1226 letters) *F Database: na_gadfly.dros *F 14,217 sequences; 24,499,473 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG13543|FBgn0034823|CT32918|FBan0013543 last_updated:000321 4232 3.4e-187 1 *F CG15137|FBgn0032628|CT35034|FBan0015137 last_updated:000321 250 0.0036 1 *F >CG13543|FBgn0034823|CT32918|FBan0013543 last_updated:000321 *F Length = 2046 *F Plus Strand HSPs: *F Score = 4232 (635.0 bits), Expect = 3.4e-187, P = 3.4e-187 *F Identities = 848/850 (99%), Positives = 848/850 (99%), Strand = Plus / Plus *F Query: 175 ATGCGAAAAAGTGTGGTGAACGTCATGTGGAAGTATGCCAATTCCCTTCACGAATACGTG 234 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 ATGCGAAAAAGTGTGGTGAACGTCATGTGGAAGTATGCCAATTCCCTTCACGAATACGTG 60 *F Query: 235 TTCCTGGTCATAGCCGGCTTTCGCGTAGTCTGTGTCTTTCTGGAGCTGGTCAGTCGATGG 294 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 TTCCTGGTCATAGCCGGCTTTCGCGTAGTCTGTGTCTTTCTGGAGCTGGTCAGTCGATGG 120 *F Query: 295 TCGCAGCGTCGCACCTTTGTGAGGCTCTTCAACTCATTCCGGAGACTGTATCAGAGAAAT 354 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 TCGCAGCGTCGCACCTTTGTGAGGCTCTTCAACTCATTCCGGAGACTGTATCAGAGAAAT 180 *F Query: 355 CCGGATATAATCCAGTACTGCCGAAGGAGCATCGTTAGCAAATTCTTTTGCGTCACAATG 414 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 CCGGATATAATCCAGTACTGCCGAAGGAGCATCGTTAGCAAATTCTTTTGCGTCACAATG 240 *F Query: 415 ACAGAGACACTGCACATCATAGTCACCTTGGCCATGATGAGGAATCGGCTGAGCATTGCT 474 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 ACAGAGACACTGCACATCATAGTCACCTTGGCCATGATGAGGAATCGGCTGAGCATTGCT 300 *F Query: 475 CTGGCTCTGCGCATTTGGGCCGTATTGAGTCTGACGGCCATAATAAATGTAATCATCACG 534 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301 CTGGCTCTGCGCATTTGGGCCGTATTGAGTCTGACGGCCATAATAAATGTAATCATCACG 360 *F Query: 535 CAGTACTATGTGGCCACCGCGTGTGTGCGAGGACGATATGCGCTCCTGAACAAGGATCTT 594 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 361 CAGTACTATGTGGCCACCGCGTGTGTGCGAGGACGATATGCGCTCCTGAACAAGGATCTT 420 *F Query: 595 CAGGCGATTGTGACCGAATCCCAGTCGCTGGTTCCCAACGGAGGTGGCGTCTTTGTGACC 654 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 421 CAGGCGATTGTGACCGAATCCCAGTCGCTGGTTCCCAACGGAGGTGGCGTCTTTGTGACC 480 *F Query: 655 AAGTGTTGCTACCTAGCGGATCGCTTGGAGCGAATAGCCAAGTCCCAGTCGGACCTACAG 714 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 481 AAGTGTTGCTACCTAGCGGATCGCTTGGAGCGAATAGCCAAGTCCCAGTCGGACCTACAG 540 *F Query: 715 GAGCTCGTCGAAAACTTGTCCACGGCATACGAAGGAGAAGTGGTCTGCCTGGTCATCACA 774 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 541 GAGCTCGTCGAAAACTTGTCCACGGCATACGAAGGAGAAGTGGTCTGCCTGGTCATCACA 600 *F Query: 775 TACTATCTGAATATGCTGGGCACCTCGTATCTGCTGTTCAGCATTAGCAAGTATGGCAAT 834 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 601 TACTATCTGAATATGCTGGGCACCTCGTATCTGCTGTTCAGCATTAGCAAGTATGGCAAT 660 *F Query: 835 TTTGGGAATAACCTGCTCGTGATCATCACTCTTTGTGGCATTGTCTACTTCGTATTTTAC 894 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 661 TTTGGGAATAACCTGCTCGTGATCATCACTCTTTGTGGCATTGTCTACTTCGTATTTTAC 720 *F Query: 895 GTCGTCGATTGCTGGATCAACGCGTTTAATGTGTTTTACCTTTTGGATGCCCATGATAAG 954 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 721 GTCGTCGATTGCTGGATCAACGCGTTTAATGTGTTTTACCTTTTGGATGCCCATGATAAG 780 *F Query: 955 ATGGTTAAGTTGCTGAATAAGCGAACTTTGTTTCAGCCAGGTCTGGATCATCGATTGGAA 1014 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 781 ATGGTTAAGTTGCTGAATAAGCGAACTTTGTTTCAGCCAGGTCTGGATCATCGATTGGAA 840 *F Query: 1015 ATGGTTGTAA 1024 *F |||||| || *F Sbjct: 841 ATGGTTAGAA 850 *F 2. GR59D.2 *F \---------- *F Query= GR59D.2,AC006245,70261-71505, 1245 bases, 10CA15D6 checksum. *F (1245 letters) *F Database: na_gadfly.dros *F 14,217 sequences; 24,499,473 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG13543|FBgn0034823|CT32918|FBan0013543 last_updated:000321 5176 1.5e-260 2 *F CG17784|FBgn0039192|CT33005|FBan0017784 last_updated:000321 190 0.85 1 *F >CG13543|FBgn0034823|CT32918|FBan0013543 last_updated:000321 *F Length = 2046 *F Plus Strand HSPs: *F Score = 5176 (776.6 bits), Expect = 1.5e-260, Sum P(2) = 1.5e-260 *F Identities = 1036/1037 (99%), Positives = 1036/1037 (99%), Strand = Plus / Plus *F Query: 1 ATGGTTGACTTGGTGAAGACGATTTTGCTCATTGCCTACTGGTATGGCCTTGCCGTGGGA 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 853 ATGGTTGACTTGGTGAAGACGATTTTGCTCATTGCCTACTGGTATGGCCTTGCCGTGGGA 912 *F Query: 61 GTGTCCAACTTCGAGGTGGACTGGCTAACTGGAGAAGCCATTGCCACCCGAAGGACTACG 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 913 GTGTCCAACTTCGAGGTGGACTGGCTAACTGGAGAAGCCATTGCCACCCGAAGGACTACG 972 *F Query: 121 ATCTATGCAGCAGTGCATAATGCCAGCCTTATCACTCTGCTGATTCTTTTCAATCTTGGC 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 973 ATCTATGCAGCAGTGCATAATGCCAGCCTTATCACTCTGCTGATTCTTTTCAATCTTGGC 1032 *F Query: 181 AATAACTCACTGAAATCCGAGTTCATAAGTGCTCGATATCTGCATGAGTACTTCTTTATG 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1033 AATAACTCACTGAAATCCGAGTTCATAAGTGCTCGATATCTGCATGAGTACTTCTTTATG 1092 *F Query: 241 CTCATGACTGCGGTTCGAATCTCGGCAGTTCTGCTCTCACTGATAACCAGATGGTATCAG 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1093 CTCATGACTGCGGTTCGAATCTCGGCAGTTCTGCTCTCACTGATAACCAGATGGTATCAG 1152 *F Query: 301 CGTTCCAGATTCATTCGAATTTGGAATCAGATACTAGCCCTAGTTCGCGATAGACCTCAA 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1153 CGTTCCAGATTCATTCGAATTTGGAATCAGATACTAGCCCTAGTTCGCGATAGACCTCAA 1212 *F Query: 361 GTGGTTCGTGGGCGCTGGTATCGTCGCAGCATTATCCTTAAATTTGTGTTCTGTGTCCTG 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1213 GTGGTTCGTGGGCGCTGGTATCGTCGCAGCATTATCCTTAAATTTGTGTTCTGTGTCCTG 1272 *F Query: 421 TCAGATTCTCTGCACACCATATCGGATGTGAGTGCGCAACGAAAGCGGATCACTGCTGAC 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1273 TCAGATTCTCTGCACACCATATCGGATGTGAGTGCGCAACGAAAGCGGATCACTGCTGAC 1332 *F Query: 481 CTGATTGTCAAACTGAGCTTACTGGCCACACTGACCACCATTTTTAACATGATCGTGTGC 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1333 CTGATTGTCAAACTGAGCTTACTGGCCACACTGACCACCATTTTTAACATGATCGTGTGC 1392 *F Query: 541 CAGTACTACTTGGCCATGGTGCAGGTGATTGGGCTCTACAAGATTCTGCTCCAAGATCTG 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1393 CAGTACTACTTGGCCATGGTGCAGGTGATTGGGCTCTACAAGATTCTGCTCCAAGATCTG 1452 *F Query: 601 CGATGCTTGGTGCGCCAAGCTGAATGCATCTGCTCCATTCGCAATCGGCGAGGTGGAGTT 660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1453 CGATGCTTGGTGCGCCAAGCTGAATGCATCTGCTCCATTCGCAATCGGCGAGGTGGAGTT 1512 *F Query: 661 TACTCCATTCAGTGCTGCTCGTTGGCAGATCAGCTGGATCTAATTGCCGAAAGGCATTAC 720 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1513 TACTCCATTCAGTGCTGCTCGTTGGCAGATCAGCTGGATCTAATTGCCGAAAGGCATTAC 1572 *F Query: 721 TTTCTGAAGGACAGACTTGATGAGATGTCGGACCTTTTCCAGATACAGAGCCTAAGCATG 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1573 TTTCTGAAGGACAGACTTGATGAGATGTCGGACCTTTTCCAGATACAGAGCCTAAGCATG 1632 *F Query: 781 AGCCTGGTGTACTTTTTCTCCACCATGGGCTCCATCTACTTTAGCGTCTGTTCGATCCTG 840 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1633 AGCCTGGTGTACTTTTTCTCCACCATGGGCTCCATCTACTTTAGCGTCTGTTCGATCCTG 1692 *F Query: 841 TACAGCTCCACAGGATTCGGCTCTACATACTGGGGTCTTCTGCTGATTGTACTATCCACG 900 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1693 TACAGCTCCACAGGATTCGGCTCTACATACTGGGGTCTTCTGCTGATTGTACTATCCACG 1752 *F Query: 901 GCTTCCTTCTACATGGACAATTGGTTGTCCGTTAACATTGGGTTTCATATTCGAGATCAG 960 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1753 GCTTCCTTCTACATGGACAATTGGTTGTCCGTTAACATTGGGTTTCATATTCGAGATCAG 1812 *F Query: 961 CAGGACGAACTATTCCGAGTGCTGGCGGATCGAACTCTGTTCTATCGGGAATTGGACAAC 1020 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1813 CAGGACGAACTATTCCGAGTGCTGGCGGATCGAACTCTGTTCTATCGGGAATTGGACAAC 1872 *F Query: 1021 CGACTGGAGGCAGCCGT 1037 *F ||||||||||||||| | *F Sbjct: 1873 CGACTGGAGGCAGCCTT 1889 *F Score = 735 (110.3 bits), Expect = 1.5e-260, Sum P(2) = 1.5e-260 *F Identities = 147/147 (100%), Positives = 147/147 (100%), Strand = Plus / Plus *F Query: 1099 TTTGAGAACTTCCAACTGCAACTGGCCAGTAACCGGCATGAATTCTACGTTATGGGTCTC 1158 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1888 TTTGAGAACTTCCAACTGCAACTGGCCAGTAACCGGCATGAATTCTACGTTATGGGTCTC 1947 *F Query: 1159 TTTAAAATGGAACGTGGTCGTCTAATCGCTATGCTAAGCTCAGTGATCACTCATACTATG 1218 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1948 TTTAAAATGGAACGTGGTCGTCTAATCGCTATGCTAAGCTCAGTGATCACTCATACTATG 2007 *F Query: 1219 GTTCTTGTTCAGTGGGAAATTCAAAAC 1245 *F ||||||||||||||||||||||||||| *F Sbjct: 2008 GTTCTTGTTCAGTGGGAAATTCAAAAC 2034 # *U FBrf0135279 *a O'Farrell *b P. *t 2001.4.12 *T personal communication to FlyBase *u FlyBase error report for CG3183 on Thu Apr 12 14:54:41 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 12 22:54:49 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 12 Apr 2001 22:54:49 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 12 Apr 2001 14:54:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ofarrell@cgl.ucsf.edu *F Subject: FlyBase error report for CG3183 on Thu Apr 12 14:54:41 2001 *F Content-Length: 736 *F Error report from Patrick O'Farrell (ofarrell@cgl.ucsf.edu) *F Gene or accession: CG3183 *F Release: 1 *F Missed gene *F Comments: CG3183 is described as a motor protein presumably based only on *F homology. I think this is incorrect and that it is a homolog of the cell *F cycle regulator geminin. This Drosophila sequence turns up in BLAST searches *F starting with the mammalian geminin sequences and, reciprocally, the fly *F sequence pulls out the mammalian geminins as homologs. A region of homology *F to dynien suggests that there might a weaker relationship here. However, the *F fly protein and the mammalian geminins are all small and form a tighter group. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0135280 *a Millburn *b G. *t 2001.4.17 *T personal communication to FlyBase *u FlyBase error report for CG13411 on Tue Apr 17 04:30:08 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 17 12:30:08 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Apr 2001 12:30:08 \+0100 *F Date: Tue, 17 Apr 2001 04:30:08 \-0700 (PDT) *F From: FlyBase-error@hedgehog.lbl.gov *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG13411 on Tue Apr 17 04:30:08 2001 *F Content-Length: 885 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG13411 *F Release: 1 *F Gene annotation error *F Gene CG13411 should be split. *F Comments: Clyne et al., 2000, Science 287(5459): 1830--1834 (FBrf0126794) *F describe 2 *F gustatory receptor genes, GR93F.2 (FBgn0041228) and GR93F.3 (FBgn0041227) *F whose sequences match different regions of CG13411 (FBgn0038907), suggesting *F either that CG13411 needs splitting into 2 separate genes, or (given that the *F sequences of GR93F.2 and GR93F.3 in FBrf0126794 are said to be partial) that *F there is only one gene and GR93F.2 and GR93F.3 need merging, *F Sequences of GR93F.2 and GR93F.3 (worked out from coordinates given in *F References and Notes of FBrf0126794) and alignments to CG13411 follow *F in e-mail to flybase-updates, *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From gm119@gen.cam.ac.uk Tue Apr 17 12:32:17 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Apr 2001 12:32:17 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: CG13411 alignments *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 17 Apr 2001 12:32:13 \+0100 *F Content-Length: 3586 *F sequence of GR93F.2 (from FBrf0126794): *F (coordinates in sequence header are relative to the clone sequence as *F of 5 January 2000) *F (partial sequence) *F >GR93F.2,AC012892,2781-2650 *F ctgtactagaaagactagataggtcaccattgccgagagaaagaacagggtcagttcgttggagacctcaaagaggccc *F aaaggaaacactcgcagctcctggtgttgaaggcggcccaggaaaagctccag *F BLASTN against CGs: *F Query= GR93F.2,AC012892,2781-2650, 132 bases, E2A12F30 checksum. *F (132 letters) *F Database: na_gadfly.dros *F 14,217 sequences; 24,499,473 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG13411|FBgn0038907|CT32767|FBan0013411 last_updated:000321 660 9.8e-25 1 *F CG13714|FBgn0040701|CT33180|FBan0013714 last_updated:000321 114 0.93 1 *F >CG13411|FBgn0038907|CT32767|FBan0013411 last_updated:000321 *F Length = 1995 *F Minus Strand HSPs: *F Score = 660 (99.0 bits), Expect = 9.8e-25, P = 9.8e-25 *F Identities = 132/132 (100%), Positives = 132/132 (100%), Strand = Minus / Plus *F Query: 132 CTGGAGCTTTTCCTGGGCCGCCTTCAACACCAGGAGCTGCGAGTGTTTCCTTTGGGCCTC 73 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1837 CTGGAGCTTTTCCTGGGCCGCCTTCAACACCAGGAGCTGCGAGTGTTTCCTTTGGGCCTC 1896 *F Query: 72 TTTGAGGTCTCCAACGAACTGACCCTGTTCTTTCTCTCGGCAATGGTGACCTATCTAGTC 13 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1897 TTTGAGGTCTCCAACGAACTGACCCTGTTCTTTCTCTCGGCAATGGTGACCTATCTAGTC 1956 *F Query: 12 TTTCTAGTACAG 1 *F |||||||||||| *F Sbjct: 1957 TTTCTAGTACAG 1968 *F sequence of GR93F.3 (from FBrf0126794): *F (coordinates in sequence header are relative to the clone sequence as *F of 5 January 2000) *F (partial sequence) *F >GR93F.3,AC012892,4271-4143 *F ctgaaccacataggtaaagtaggtgatcatacttgacagaaacagcaagatgacctcgttggatacttcgaatagtccc *F aagggtctaacgcgaaactcgaagaagttcagacggcttaagaacatttc *F BLASTN against CGs: *F Query= GR93F.3,AC012892,4271-4143, 129 bases, C9A98456 checksum. *F (129 letters) *F Database: na_gadfly.dros *F 14,217 sequences; 24,499,473 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG13411|FBgn0038907|CT32767|FBan0013411 last_updated:000321 640 8.1e-24 1 *F >CG13411|FBgn0038907|CT32767|FBan0013411 last_updated:000321 *F Minus Strand HSPs: *F Score = 640 (96.0 bits), Expect = 8.1e-24, P = 8.1e-24 *F Identities = 128/128 (100%), Positives = 128/128 (100%), Strand = Minus / Plus *F Query: 129 GAAATGTTCTTAAGCCGTCTGAACTTCTTCGAGTTTCGCGTTAGACCCTTGGGACTATTC 70 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1039 GAAATGTTCTTAAGCCGTCTGAACTTCTTCGAGTTTCGCGTTAGACCCTTGGGACTATTC 1098 *F Query: 69 GAAGTATCCAACGAGGTCATCTTGCTGTTTCTGTCAAGTATGATCACCTACTTTACCTAT 10 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1099 GAAGTATCCAACGAGGTCATCTTGCTGTTTCTGTCAAGTATGATCACCTACTTTACCTAT 1158 *F Query: 9 GTGGTTCA 2 *F |||||||| *F Sbjct: 1159 GTGGTTCA 1166 # *U FBrf0135281 *a Riesgo-Escovar *b J. *t 2001.2.7 *T personal communication to FlyBase *u *F From riesgo@calli.cnb.unam.mx Thu Feb 08 02:31:33 2001 *F To: rd120@gen.cam.ac.uk *F Subject: flp and chico (more than a bit late....) *F Dear Rachel: *F Sorry for answering so late, but I have not looked at my old email *F account for some time. ... I think the text is OK, so it can be *F archived... *F Sincerely: *F Juan R. Riesgo. *F >Dear Juan, *F > *F >Ernst has been in touch about the flp and chico situation in FlyBase. *F >I enclose my reply to him below so that you can see where we have got *F >to. I am writing to you to ask whether I could archive your *F >information about the complementation data to add to the information we *F >have about the 'fs(2)4' mutation, because it is this that separates flp *F >from chico. All you would need to do would be to say that I could *F >archive the following text as a personal communication from you to *F >FlyBase. *F > *F >---------------------------------------------------------------- *F >I do not think that chico (fs(2)4) is allelic to flp since it *F >complements the flphd allele. fs(2)4 flies do not show unexpanded *F >wings, are not dark or are male sterile. For these reasons we decided *F >that the best would be to call it the neutral name fs(2)4, since it was *F >not at all clear in my mind that the mutation was an allele of flp. *F >---------------------------------------------------------------- *F > *F >Of course feel free to modify the text as you wish, I only make this *F >suggestion to give you the option of doing almost no extra work! *F > *F >With best wishes, *F > *F >Rachel. *F > *F >---------------------------------------------------------------------- *F >Rachel Drysdale, Ph.D. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: rd120@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- # *U FBrf0135282 *a Van Doren *b M. *c R. *d Lehmann *t 2001.2.14 *T personal communication to FlyBase *u *F Mime-Version: 1.0 *F Date: Wed, 14 Feb 2001 15:25:45 \-0500 *F To: Beverley Matthews , Mark Vandoren *F *F From: Ruth Lehmann *F Subject: Re: Question about Hmgcr mutations *F Dear Beverly, *F enclosed is a wordfile with the clb/hmgcr alleles, their aa changes and *F annotations about their respective strength if available. I have given the *F alleles numbers (clb1-13) and would like to ask you to keep those numbers *F as they refer to alleles we have in our stock collection and to our *F published data (Natur 98). I noticed that you have two P element alleles *F 01152 and C14.5, this is the same P-element, it is likely a hypomorph as it *F produces a weak germ cell migration defect. All this information should be *F cited as Mark Van Doren and Ruth Lehmann, pers. com. Please let me know if *F you have further questions. Thanks. *F best wishes, ruth lehmann. *F > Dear Dr. Lehmann, *F > *F > I am a curator for FlyBase and am working on the annotation of the *F > Hmgcr gene. I would like to include the sites of the 4 nonsense and 7 *F > missense mutations mentioned in your 1998 Nature 396(6710):466-469 *F > paper. Unfortunately, the specific locations of the mutations are not *F > provided. If you or your colleagues can send me the amino acid *F > locations of the mutations, I'll include them in the annotation. The *F > information will be cited as a personal communication from you to *F > FlyBase. Thanks for your help. *F > *F > Sincerely, *F > *F > Beverley Matthews *F > Curator, FlyBase Harvard *F \------------------------------------------------------------------------------ *F dHMGR Allele Sequence Data *F Personal communication by Mark Van Doren and Ruth Lehmann *F reference Van Doren et al Nature 396, 466-469, (1998) *F All bp numbers refer to pNB36C cDNA. *F All aa numbers refer to translation of pNB36C *F Simplified allele numbers for flybase in () *F Allele \* nt Change aa Change Comment *F 11.54 (1) G841A W91STOP Strong allele , Truncation before TM *F homology region begins *F 26.31 (2) G2675A G566D Strong allele in catalytic domain *F Conserved from man to plants *F 29.29 (3) G1508A A314T-- Strong allele in TM domain *F G2405A G613S-- in catalytic domain Conserved in *F C3295A Silent man *F 111.29 (4) C1799T Q411STOP Strong allele truncates after TM *F 14.26 (5) C2123T R519STOP Strong allele *F 79.30 (6) G2740A W724STOP Deletes C-term including catalytic GQD *F 12.12 (7) G2133A R522Q Strong allele in catalytic domain *F Conserved from man to plants *F 15.25 (8) C2310T P581L in catalytic domain *F Conserved from man to plants *F 59.14 (9) G2675A D703N in catalytic domain *F Conserved in yeast and plants *F E in mammals *F 99.47 (10) G2939A G791R in catalytic domain *F Conserved from man to plants *F H4.42 (11) T2971A S801R Strong allele in catalytic domain *F Conserved from man to plants *F 102.06 (12) Same as 99.47 independent? *F 112.69 (13) G3191A G875S in catalytic domain *F Conserved man to plant *F \------------------------------------------------------------------------------ # *U FBrf0135289 *a Gibson *b M. *t 2001.2.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Feb 23 00:59:52 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GawB}c311 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Matt Gibson, University of Washington (2/01). P{GawB}c311 *F is a homozygous viable and fertile, third chromosome insertion. GAL4 is *F expressed in the eye disk peripodial epithelia and in wing disk peripodial *F and columnar epithelia. This expression pattern is described in Gibson and *F Schubiger FBrf0131300. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0135290 *a Sun *b H. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:12:51 2001 *F To: mbyhsun@ccvax.sinica.edu.tw *F Subject: Helping FlyBase: ADRC-10211 *F Dear Henry, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Molecular Analysis of the eyg-toe Gene Complex in Drosophila.' *F You mention a gene that is new to FlyBase, toe. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mbyhsun@ccvax.sinica.edu.tw Mon Mar 05 12:40:59 2001 *F Subject: Re: Helping FlyBase: ADRC-10211 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel: *F toe (twin of eyg) = CG10704 *F Best wishes, *F Henry Sun # *U FBrf0135291 *a Sunkel *b C. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:13:17 2001 *F To: cesunkel@ibmc.up.pt *F Subject: Helping FlyBase: ADRC-10230 *F Dear Claudio, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A Drosophila homologue of human Nek2: a kinase with centrosomal function.' *F You mention a gene that is new to FlyBase, Nek2. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From cesunkel@ibmc.up.pt Mon Mar 05 12:46:10 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10230 *F Rachel, *F The gene we call dmNek2 is annotated as CG17256. It is located at X7C6 *F which we have confirmed by in situ hybridisation. In GadFly it is described *F has having homology to human Nek2, NIMA and mouse Nek1. It is also *F described has having homology with cerevisiae CDC5 but this unlikely *F because the drosophila cdc5 is polo. *F If need further information let me know. *F Regards *F Claudio *F \---------------------- *F Dr.Claudio E.Sunkel *F Instituto de Biologia Molecular e Celular *F Universidad do Porto *F Rua do Campo Alegre 823 *F 4150-180 Porto *F Portugal *F Tel:+351-22-6074900 *F Fax:+351-22-6099157 # *U FBrf0135292 *a Dej *b K. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:13:37 2001 *F To: dej@wi.mit.edu *F Subject: Helping FlyBase: ADRC-10237 *F Dear Kimberley, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of mutations that affect the metaphase/anaphase transition: *F the chromosome condensation factor DCAP-G is required for chromosome *F separation at anaphase.' *F You mention a gene that is new to FlyBase, Cap-G. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From dej@wi.mit.edu Mon Mar 05 13:23:49 2001 *F Subject: RE: Helping FlyBase: ADRC-10237 *F To: 'Genetics' *F Hi Rachel, *F >You mention a gene that is new to FlyBase, Cap-G. Do you know which of the *F >Genome Project CG annotations your gene corresponds to? *F So DCap-G is the gene coding for the Drosophila homologue of the condensation *F factor XCAP-G and it corresponds to annotation CG17054. We have several *F mutations in this gene that will be presented at the Fly meeting. *F Thank you for noting this! *F Best, *F Kim *F _____________________________________________________ *F Kimberley Dej *F Whitehead Institute *F Nine Cambridge Center *F Cambridge MA 02142 *F tel: (617) 258-5246 *F fax: (617) 258-9872 *F email: dej@wi.mit.edu # *U FBrf0135293 *a Gubb *b D. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:17:34 2001 *F To: d.gubb@gen.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10330 *F Dear David, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'necrotic mutations homologous to disease-associated varients in human serpins.' *F You mention a gene that is new to FlyBase, Spn43Ad. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From dg27@mole.bio.cam.ac.uk Mon Mar 05 13:40:35 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10330 *F Rachel, *F 43Ad is a serpin in the 43A cluster that we missed from our *F sequencing or cDNAs. It was clear as a transcript between 43Ab and 43Ac, ie *F within the 5' pk intron. We have a projectile working on it, who hopefully *F will have the cDNA fully sequenced soon. At first sight it looks like a *F non-inhibitory serpin. We will let you have full details soon. *F David Gubb *F Department of Genetics *F University of Cambridge *F Downing Street *F CB2 3EH UK *F Telephone (44 or 0) 1223 333967/766488. *F fax (44 or 0) 1223 333992 *F http://www.gen.cam.ac.uk/dept/gubb.html # *U FBrf0135294 *a Spencer *b S. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:52:40 2001 *F To: sspencer@pharmdec.wustl.edu *F Subject: Helping FlyBase: ADRC-10778 *F Dear Susan, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'E(Elp)24D, an EGF-receptor inhibitor, is essential for R8 patterning.' *F You mention a gene that is new to FlyBase, E(Elp)24D. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From sspencer@pcg.wustl.edu Mon Mar 05 13:45:08 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10778 *F Dear Rachel, *F E(Elp)24D is echinoid, according to a recent paper (Bai JM, Chiu WH, Wang *F JC, Tzeng TH, Perrimon N, Hsu JC 'The cell adhesion molecule Echinoid *F defines a new pathway that antagonizes the Drosophila EGF receptor *F signaling pathway'. Development 2001;128(4):591-601). It corresponds to est *F GM09285, among others, and, as it is spread out over quite a distance on *F the chromosome, matches several CG-annotated genes (CG12676, CG16842, *F CG15424). *F Hope this is helpful, *F Cheers, *F Susan # *U FBrf0135295 *a Salz *b H. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:47:47 2001 *F To: hks@po.cwru.edu *F Subject: Helping FlyBase: ADRC-10651 *F Dear Helen, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Drosophila U2 snRNP-U2A' protein has essential functions that are *F SNF/U2B" independent.' *F You mention a gene that is new to FlyBase, U2A'. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From hks@po.cwru.edu Mon Mar 05 13:56:06 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10651 *F Good morning Rachel, *F U2A' is CG1406.....The matching up of CG numbers and splicing factors is *F published in paper by Steve Mount and myself in J. Cell Biol. (2000) *F 150:F37-F43. *F Let me know if you need any more information! *F Helen # *U FBrf0135296 *a Kellum *b R. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:36:47 2001 *F To: rkellum@pop.uky.edu *F Subject: Helping FlyBase: ADRC-10465 *F Dear Rebecca, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Genetic and Molecular Characterization of Su(var)2-5-Interacting Genes.' *F You mention a gene that is new to FlyBase, wwg. Is this a previously *F undescribed gene? What does wwg stand for? Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rkellum@pop.uky.edu Mon Mar 05 14:16:21 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10465 *F The mutant is not in the database yet, and we don't know which *F transcription unit is mutated in the mutant. The name wwg (wrinkled wing) *F is from an unpublished mutant screen of Elliott Goldstein. # *U FBrf0135297 *a Ewer *b J. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:53:19 2001 *F To: je24@cornell.edu *F Subject: Helping FlyBase: ADRC-10801 *F Dear John, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Neuroendocrine control of larval ecdysis.' *F You mention a peptide, CCAP, and I am not sure whether or not it *F corresponds to a Drosophila gene! If it does, do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. CG4910 is annotated as having a 'homolog' to 'Manduca *F sexta; gene == 'cardioacceleratory peptide 2a'' but that may be neither *F here nor there. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. Also, does your CCAP have a *F relationship to Cap-2b (FBgn0016129), or is it something completely *F different? *F Thank you for your help, *F with best wishes, *F Rachel. *F From je24@cornell.edu Mon Mar 05 14:21:02 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10801 *F Dear Rachel, *F Thank you for your note. The CCAP gene we pulled out does *F indeed correspond to CG4910, at 94C, and is the homolog of Manduca *F sexta gene 'cardioacceleratory peptide 2a' (by sequence and by *F expression pattern--I've shown that the peptide expression overlaps *F with the RNA expression). *F I hope this helps. Let me know if you need any further *F information. *F . *F yours, *F John *F John Ewer *F Cornell University, Department of Entomology *F 5130 Comstock Hall, Ithaca, NY 14853, USA *F Phone: 607-255-1395 and 607-255-1496 *F Fax 607-255-0939 *F e-mail: je24@cornell.edu # *U FBrf0135298 *a Kellum *b R. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:39:10 2001 *F To: rkellum@pop.uky.edu *F Subject: Helping FlyBase: ADRC-10510 *F Dear Rebecca, *F We are still curating the ADRC abstracts and now I am writing in *F connection with your abstract: *F 'Characterization of HOAP (HP1/ORC Associated Protein) in Drosophila *F heterochromatin formation.' *F You mention a gene that is new to FlyBase, Hoap. Do you know which of *F the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you, again, for your help, *F with best wishes, *F Rachel. *F From rkellum@pop.uky.edu Mon Mar 05 14:28:35 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10510 *F The HOAP protein is encoded by the anon fe 1G5 gene. *F Reference: Schmid, K.J. and Tautz, D. (1997). A screen for fast evolving genes *F from Drosophila. Proc. Natl. Acad. Sci. 94, 9746-9750. *F The GadFly GC desigantion for the gene is CG6219. We have not published the *F work on the HOAP protein yet. # *U FBrf0135299 *a Uemura *b T. *c R. *d Niwa *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:15:36 2001 *F To: tuemura@virus.kyoto-u.ac.jp *F Subject: Helping FlyBase: ADRC-10265 *F Dear Tadashi, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Slingshot, a Putative Dual Specific Phosphatase, Controls Reorganization of *F Actin Cytoskeleton.' *F You mention a gene that is new to FlyBase, ssh. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tuemura@virus.kyoto-u.ac.jp Mon Mar 05 14:46:02 2001 *F Subject: Re: Helping FlyBase: ADRC-10265 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F Thank you for your efforts. *F > We are currently curating the abstracts for the upcoming 42nd *F > (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F > I am writing in connection with your abstract: *F > 'Slingshot, a Putative Dual Specific Phosphatase, Controls Reorganization of *F > Actin Cytoskeleton.' *F > *F > You mention a gene that is new to FlyBase, ssh. Do you know which of the *F > Genome Project CG annotations your gene corresponds to? All the CGs *F CG6238 *F Please note that we previously named this gene 'MAP kinase *F phosphatase' tentatively simply based on its predicted amino acid sequences. *F But we have not demonstrated its activity biochemically. We would be very *F happy if FlyBase employ the new name 'slingshot'. We will make the same *F request to Genbank soon. *F Sincerely, *F with best regards, *F tadashi uemura *F ryusuke Niwa *F ===================================== *F Tadashi Uemura *F Laboratory of Molecular Genetics *F Institute for Virus Research *F Kyoto University *F Sakyo-ku, Kyoto 606-8507, Japan *F TEL: 81-75-751-4031 *F FAX: 81-75-751-3989 *F email:tuemura@virus.kyoto-u.ac.jp *F ===================================== # *U FBrf0135300 *a McCall *b K. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:11:17 2001 *F To: kmccall@bu.edu *F Subject: Helping FlyBase: ADRC-10159 and 10440 *F Dear Kim, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstracts: *F 'The role of dcp-1 in nurse cell apoptosis.' *F and *F 'Genes involved in ovarian nurse cell apoptosis.' *F You mention a gene that is new to FlyBase, nad1. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kmccall@bio.bu.edu Mon Mar 05 15:00:20 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10159 and 10440 *F Hi Rachel, *F We have just begun to characterize this gene and do not yet know what it is *F or where it maps, so we are calling it nad1 (nurse cell apoptosis *F defective-1) temporarily. *F Hope this helps, *F Kim *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Kim McCall, Ph.D. *F Assistant Professor of Biology TEL:(617)358-0442, 0441 *F Boston University FAX:(617)353-8484 *F Office: 44 Cummington St. email: kmccall@bu.edu *F Room 704 *F http://bio.bu.edu/Faculty_Staff/kmccall.html *F Mailing address: *F Boston University *F Department of Biology *F 5 Cummington St. *F Boston, MA 02215 *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ # *U FBrf0135301 *a Ayyar *b S. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:16:49 2001 *F To: sa245@mole.bio.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10313 *F Dear Savita, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'achintya and vismay: two novel, tandem-repeated TALE-class homeobox genes.' *F You mention two genes that are new to FlyBase, achintya and vismay. Do *F you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. Also \- *F we like to keep Etymology statements for genes with distinctive names. *F Would you be able to explain the origin of the achintya and vismay *F names so thatwe can explain them in FlyBase? *F Thank you for your help, *F with best wishes, *F Rachel. *F From sa245@mole.bio.cam.ac.uk Mon Mar 05 15:02:46 2001 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10313 *F Hi Rachel, *F Here is the information you requested: *F achintya: CG8819 (49A10-12 with EP(2)2107 inserted in 5'-UTR) *F vismay: CG8821 (49A10) *F As part of my project, I wanted to understand the function of Drosophila *F TGIF. A locus at 49A10 seemed to encode a batch of ESTs with a high *F degree of homology to vertebrate TGIFs. However, my initial results were *F difficult to explain. I therefore named this locus 'achintya', which is a *F Sanskrit word meaning inconceivable or beyond thought. When the genome *F sequence was published, it became clear that I was actually dealing with *F a tandem-duplication. I therefore named CG8821 as 'vismay', which in Hindi *F means surprise. *F I hope this provides the answers you're looking for. Good luck with the *F curation. *F Regards, *F Savita. *F Savita Ayyar, Research Scholar phone: 01223-333751 *F Department of Anatomy fax : 01223-333786 *F University of Cambridge, *F Cambridge CB2 3DY # *U FBrf0135302 *a McKim *b K. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:17:59 2001 *F To: mckim@rci.rutgers.edu *F Subject: Helping FlyBase: ADRC-10333 *F Dear Kim, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'An analysis of genes required for the generation and function of meiotic *F crossing over: mei-218, mei-910, and mei-1794.' *F You mention genes that are new to FlyBase, mei-910 and mei-1794. Do *F you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mckim@rci.rutgers.edu Mon Mar 05 15:17:53 2001 *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10333 *F Rachel, *F mei-910 is BG:DS02740.10. We have isolated two alleles, and there is a third *F allele from the genome work, EP(2)2115. This *F is inserted in the coding region. *F mei-1794 is CG12298. We are not set on the mei-1794 name because we are *F working to figure out if it is an allele of Dub. *F This is difficult because Dub is dominant. *F Kim *F Kim S. McKim, Ph.D. *F Waksman Institute and Department of Genetics *F Rutgers University *F 190 Frelinghuysen RD *F Piscataway NJ 08854 *F (732) 445 1164 *F mckim@rci.rutgers.edu *F http://mbclserver.rutgers.edu/~mckim # *U FBrf0135303 *a Schwendemann *b A. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:11:47 2001 *F To: aschwend@genetik.biologie.fu-berlin.de *F Subject: Helping FlyBase: ADRC-10210 *F Dear Alexander, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Piefke encodes a new member of the family of Psq-domain DNA-binding proteins.' *F You mention a gene that is new to FlyBase, pfk. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From aschwend@genetik.biologie.fu-berlin.de Mon Mar 05 15:59:39 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10210 *F dear rachel, *F here is the CG annotation corresponding to our gene: *F CG15812. *F best wishes from berlin/germany. *F yours *F alexander. # *U FBrf0135304 *a Van Doren *b M. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:43:49 2001 *F To: vandoren@jhu.edu *F Subject: Helping FlyBase: ADRC-10573 *F Dear Mark, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of fear of intimacy: A novel transmembrane protein required for *F gonad coalescence.' *F Do you know which of the Genome Project CG annotations your foi gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From vandoren@jhu.edu Mon Mar 05 16:55:41 2001 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10573 *F Rachel, *F fear of intimacy (foi) is CG6817 *F Best, *F Mark *F Mark Van Doren, PhD. *F Assistant Professor *F Department of Biology *F Johns Hopkins University *F 137 Mudd Hall *F 3400 N. Charles St. *F Baltimore, MD 21218 *F 410-516-4717 Office *F 410-516-4830 Lab *F 410-516-4794 Fax # *U FBrf0135305 *a Burtis *b K. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:50:46 2001 *F To: kcburtis@ucdavis.edu *F Subject: Helping FlyBase: ADRC-10729 *F Dear Ken, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of the SNM1 gene of Drosophila melanogaster.' *F You mention a gene that is new to FlyBase, Snm1. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kcburtis@ucdavis.edu Mon Mar 05 17:23:58 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10729 *F Hi Rachel, *F The gene is CG10018 (CT9654). The name is by homology to the S. *F cerevisiae SNM1 gene (also known as PSO2), and the homology is quite *F obvious and was of course picked up in the annotation. I believe we *F are the first to find a mutant allele. *F See you in Washington, *F Ken # *U FBrf0135306 *a Tomkiel *b J. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:21:22 2001 *F To: jtomkiel@cmb.biosci.wayne.edu *F Subject: Helping FlyBase: ADRC-10426 *F Dear John, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Molecular characterization of teflon, a gene required for maintenance of *F autosomal pairing at meiosis I in male Drosophila melanogaster.' *F You mention a gene that is new to FlyBase, teflon. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. I wonder whether the *F gene we have in FlyBase as mei-S8 (FBgn0002713) might be the same thing *F as teflon \- you will certainly know, judging from the publications for *F mei-S8. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jtomkiel@cmb.biosci.wayne.edu Mon Mar 05 18:02:39 2001 *F Subject: Re: Helping FlyBase: ADRC-10426 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel: *F The teflon gene corresponds to CG8961. We hope to be publishing a *F detailed molecular characterization of the mutants within the next few *F months \- I can send you a preprint when its ready if it will help with the *F annotation. *F The teflon mutatns very likely identify the same gene as the meiS8 *F mutants, but since there are no longer any alleles of meiS8 in existance it *F was impossible to test by complementation. Both mutations map to *F approximately 80.0 on 2R. The reported phenotype for meiS8 is similar to *F that of teflon in that both mutations cause high rates of fourth chromosome *F nondisjunction in male meiosis and are male-specific. The teflon mutations *F also cause nondisjunction of the major autosomes. This was not observed for *F meiS8, but was not specifically tested genetically. The reported *F cytological examination of mei-S8 describes 4th chromosome nd but not major *F autosome nd. Since in teflon, the meiotic phenotype is quite striking, this *F suggests that the teflon mutants may differ from meiS8 in this respect. *F However, the original cytological description suggest that the observations *F were cursory and not very thorough. Another argument that tef and meiS8 are *F the same is that 5 alleles of tef came out of the Zuker lab screen, but no *F other mutations with a similar phenotype were found. In short, my guess is *F that tef and meiS8 identify the same gene, but we'll never know for sure. *F I hope this helps. *F Sincerely, *F Asst. Professor *F Center for Molecular Medicine and Genetics *F Wayne State University *F BSB 5117 *F Detroit, MI *F 313-577-1976 *F \---------- # *U FBrf0135307 *a Clark *b A. *t 2001.3.5 *T personal communication to FlyBase *u *F From c92@psu.edu Tue Mar 06 17:18:39 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10569 *F Rachel, *F I talked to Bernardo Carvalho about PRY, and he said that *F PCR products from the primers in the 5' part and 3' part of the gene *F unambiguously map in kl-3 and kl-5. Thus we DO know that the kl-5 *F fertility factor is distal to PRY, and that PRY is between the two dynein *F genes that constitute the kl-5 and kl-3 fertility factors. Another paper *F was submitted on additional Y-linked genes. These were all in the unmapped *F 'arm-U' of the whole genome shotgun assembly, so they don't have CG numbers. *F Andy *F Andrew G. Clark *F Institute of Molecular Evolutionary Genetics *F 208 Mueller Laboratory *F Department of Biology *F Pennsylvania State University *F University Park, PA 16802 USA *F TEL: 814-863-3891 *F FAX: 814-865-9131 *F email: c92@psu.edu *F http://www.bio.psu.edu/people/faculty/clark/ # *U FBrf0135308 *a Smith *b M. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:07:58 2001 *F To: courey@chem.ucla.edu *F Subject: Helping FlyBase: ADRC-10095 *F Dear Albert, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Smt3-conjugation pathway and its role in regulating Dorsal function.' *F You mention a gene that is new to FlyBase, Ulp1. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From msmith@chem.ucla.edu Mon Mar 05 19:36:06 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10095 *F Dr. Drysdale, *F The Ulp1 gene corresponds to CG12359. *F Matthew Smith # *U FBrf0135309 *a Chern *b J. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:04:16 2001 *F To: kchoi@bcm.tmc.edu *F Subject: Helping FlyBase: ADRC-10952 *F Dear Kwang-Wook, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Cloning and Characterizing of Lobe mutation.' *F >From your abstract it looks like you know which of the Genome Project *F CG annotations your genes correspond to, in which case please could you *F let usknow which one it is. All the CGs have corresponding gene *F records in FlyBase already and we don't like to make duplicate records *F for what is actually the same gene unless we can't avoid it. If your *F genes do not correspond to CGs then perhaps you could tell me their map *F location, as this is valuable information for the genome annotation *F project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From jc691586@express.ssctr.bcm.tmc.edu Mon Mar 05 19:50:43 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10952 *F Hi, it's CG10109. *F Josh # *U FBrf0135310 *a Orr *b W. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:50:02 2001 *F To: BORR@mail.smu.edu *F Subject: Helping FlyBase: ADRC-10709 *F Dear William, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Peroxiredoxin Gene Family in Drosophila melanogaster.' *F You mention genes that are new to FlyBase, Px4156, Px4783, Px5037, *F Px2540 and Px6005. Do you know which of the Genome Project CG *F annotations your genes correspond to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F If any of your genes do not correspond to a CG then perhaps you could *F tell me their map location, as this is valuable information for the *F genome annotation project. Also, you say 'peroxidredoxin (Prx) genes' *F and then give them symbols such as DPx4156, DPx4783 and DPx5037 (though *F we must drop the D for Drosophila hence they will be going in the *F database as Px4156, Px4783 and Px5037 etc). If you would prefer them *F to be Prx4156, Prx4783 and Prx5037 etc in FlyBase, now would be a good *F time to say so. *F Thank you for your help, *F with best wishes, *F Rachel. *F From borr@mail.smu.edu Mon Mar 05 20:44:46 2001 *F Subject: Re: Helping FlyBase: ADRC-10709 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F If the D needs to be dropped, then by all means I would prefer the *F designation Prx rather than Px, to denote the individual peroxiredoxin *F genes. All of these genes have CGs and are listed below: *F Prx2540 CG11765 and CG12405 *F Prx4156 CG1274 *F Prx5037 CG5826 *F Prx4783 CG1633 *F Prx6005 CG3083 *F Best regards, Bill # *U FBrf0135311 *a Wernet *b M. *t 2001.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:49:41 2001 *F To: mathias.wernet@nyu.edu *F Subject: Helping FlyBase: ADRC-10700 *F Dear Mathias, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identifying genes involved in opsin regulation and photoreceptor cell *F type specification using a Gal4 enhancer trap screen.' *F You mention two genes that are new to FlyBase, P10 and P53. Do you *F know which of the Genome Project CG annotations your genes correspond *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If your genes do not correspond to a CG *F then perhaps you could tell me their map locations, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mathias.wernet@nyu.edu Mon Mar 05 21:12:42 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10700 *F Hi Rachel, *F >You mention two genes that are new to FlyBase, P10 and P53. Do you *F >know which of the Genome Project CG annotations your genes correspond *F >to? *F In the meantime I rescued genomic DNA flanking these two P-elements and it *F turned out that I targeted two already known genes: P53 turned out to be *F homothorax and P10 is elbow. Maybe, to be precise I should say elbowB? *F Originally it was thought that there are two genes elA and elB, but \- if I *F am well informed, the genomic sequence revealed only one transcript. I *F don't know if it should be called elbowA, B or simply elbow...? *F thank you, *F Mathias. *F \----------------------------------------------------------------------- *F Mathias Wernet *F Dept. Biology *F NYU *F 1009 Main Building *F 100 Washington Square East *F New York NY 10003-6688 *F Lab: (212) 998 3966 *F FAX: (212) 995 4710 *F home: (646) 6546845 *F e-mail: mathias.wernet@nyu.edu *F From rd120@gen.cam.ac.uk Wed Mar 07 16:10:29 2001 *F To: mathias.wernet@nyu.edu *F Subject: Re: Helping FlyBase: ADRC-10700 *F Dear Matthias, *F Thanks for the information \- I will use your mail as a personal *F communication from you to FlyBase to serve as the source for the info. *F >and P10 is elbow. Maybe, to be precise I should say elbowB? *F >Originally it was thought that there are two genes elA and elB, but \- if I *F >am well informed, the genomic sequence revealed only one transcript. I *F >don't know if it should be called elbowA, B or simply elbow...? *F If you mean your P10 maps to CG4220 then we should say elB. However *F the fact the first round of annotation did not assign a CG to elA *F should not be taken as evidence that an elA transcript does not exist. *F I'll link P10 to elB unless I hear from you to do otherwise. *F Many thanks for your help, *F With best wishes, *F Rachel. # *U FBrf0135312 *a Ferrandon *b D. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:10:46 2001 *F To: D.Ferrandon@ibmc.u-strasbg.fr *F Subject: Helping FlyBase: ADRC-10154 *F Dear Dominique, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Genetic analysis of the Drosophila innate immune response.' *F You mention two genes that are new to FlyBase, shadok and galere. Do *F you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From D.Ferrandon@ibmc.u-strasbg.fr Tue Mar 06 08:18:37 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10154 *F Dear Rachel, *F shadok and galere are two genes found in our screen. Even though the shadok *F mutations did complement immune deficiency (imd) in our reporter assay, we *F found that shadok and immune deficiency affect the same gene (BG5 in BDGP) *F and I guess they should all be called imd. The cloning of the imd mutation *F has been performed by P. Georgel and Sylvia Naiza working with Jean-Marc *F Reichhart in Jules Hoffmann's lab. *F Regarding galere, we are currently trying to clone it. However, since we *F are collaborating with a private partner, we are bound by a nondisclosure *F clause in our contract and therefore I am not allowed to tell you where it *F maps. However, as soon as I'll get the authorization, I will let you know *F what it is. Sorry about this. *F Best wishes *F Dominique # *U FBrf0135313 *a Tricoire *b H. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:51:39 2001 *F To: tricoire@ijm.jussieu.fr *F Subject: Helping FlyBase: ADRC-10741 *F Dear Herve, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Oxydative stress resistance, ageing and retinal neurodegeneration in *F D. melanogaster.' *F You mention a gene that is new to FlyBase, UY530. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tricoire@ijm.jussieu.fr Tue Mar 06 13:05:59 2001 *F To: ''Rachel Drysdale (Genetics)'' *F Subject: RE: Helping FlyBase: ADRC-10741 *F Dear Rachel, *F Indeed UY530 is a PUAS insertion close to the gene referred as CG4026 and *F CG13122 which encode an inositol triphosphate kinase and should be on the same *F strand. *F Best wishes *F Hervé TRICOIRE # *U FBrf0135314 *a Nastase *b K. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:48:32 2001 *F To: bio_cals@jhu.edu *F Subject: Helping FlyBase: ADRC-10676 *F Dear Allen, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Missing Imaginal Precursors: A trithorax Group Gene with Possible Roles in *F Imaginal Cell Proliferation.' *F You mention a gene that is new to FlyBase, mip. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From nastase@jhu.edu Tue Mar 06 14:25:39 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10676 *F Hi Rachel, *F Allen forwarded me an email regarding my poster and information for *F the upcoming Drosophila conference. The gene I am working on, mip, *F corresponds to CG4539, which is located at 30E. *F My address and information are as follows: *F Kristin K. Nastase *F Johns Hopkins Univeristy *F Mudd Hall *F 3400 N. Charles St. *F Baltimore, MD 21218 *F 410-516-6543 *F nastase@jhu.edu *F Flybase and the conference seem to be very organized!! In a world *F that often seems chaotic, it is pleasant to experience some sort of *F organization. *F Thanks, *F Kristin # *U FBrf0135315 *a Orlichenko *b L. *t 2001.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:15:15 2001 *F To: jotousa@nd.edu *F Subject: Helping FlyBase: ADRC-10261 *F Dear Joseph, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila tetracycline transporter acts in the secretory pathway.' *F You mention a gene that is new to FlyBase, rtet. Do you know which of *F the Genome Project CG annotations your gene corresponds to? Possible *F one of CG5760, CG5078 and CG17637... All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F If your gene does not correspond to a CG then perhaps you could tell me *F its map location, as this is valuable information for the genome *F annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From lorliche@nd.edu Tue Mar 06 16:14:22 2001 *F To: rd120@gen.cam.ac.uk *F Cc: 'Joe O'Tousa' *F Subject: RE: FWD: Helping FlyBase: ADRC-10261 *F Dear Rachel Drysdale, *F Drtet gene coresponds to CG5760. Please note that the gene is named Drtet, not *F rtet. *F Lidiya Orlichenko. *F Ph.D.Candidate *F Department of Biological Sciences *F Notre Dame, IN 46556 *F Phone (219) 631-4168 *F e-mail lorliche@nd.edu # *U FBrf0135316 *a Dansereau *b D. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 06 09:33:10 2001 *F To: ddansere@ualberta.ca *F Subject: Helping FlyBase: ADRC-10921 *F Dear David, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'ectopic margin (ema) is required for refinement of Notch activity at the D/V *F boundary of the wing.' *F You mention a gene that is new to FlyBase, ema. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. Also, if the P insertions are from the *F BDGP gene disruption projects then I would be grateful if you could let *F me know the identifier numbers, so that I can keep all the relevent *F bits and pieces in FlyBase tied together. *F Thank you for your help, *F with best wishes, *F Rachel. *F From ddansere@ualberta.ca Tue Mar 06 16:44:31 2001 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10921 *F Rachel *F ema maps to 100F, l(3)03429. This maps in the middle of the PTB *F transcription unit but we are still working on the experiments to show *F that ema encodes PTB. *F The PTB trascription unit is complex based on EST and cDNA sequences and *F it includes CG2094 and CG2290. Again, we have not shown that PTB is *F disrupted by ema alleles but we do know that l(3)03429 is an allele of *F ema. *F Contact me for any clarifiaction, *F David *F ____________________________________________________________________ *F David A. Dansereau, Ph.D. candidate *F Molecular Biology and Genetics, University of Alberta *F ____________________________________________________________________ # *U FBrf0135317 *a MacMullin *b A. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:18:39 2001 *F To: jacobsr@mcmaster.ca *F Subject: Helping FlyBase: ADRC-10345 *F Dear Roger, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila velis/lin-7 expression in neurons suggests function in *F organization of the post-synaptic membrane.' *F You mention a gene that is new to FlyBase,veli. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From macmulaa@mcmaster.ca Tue Mar 06 16:53:52 2001 *F Subject: Re: Helping FlyBase: ADRC-10345 *F To: *F Rachel, *F Drosophila veli corresponds to gene annotation CG7662, at 96B15-17. *F Glad to be of help, *F Allison MacMullin # *U FBrf0135318 *a Zhou *b R. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:48:09 2001 *F To: rzhou@fas.harvard.edu *F Subject: Helping FlyBase: ADRC-10671 *F Dear Rui, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Composition and activation of the Drosophila IKK complex.' *F You mention a gene that may be new to FlyBase, Uev1A. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rzhou@fas.harvard.edu Tue Mar 06 19:14:16 2001 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10671 *F Dear Rachel, *F The corresponding ID# for dUev1A is CG10640. *F With best wishes, *F Rui # *U FBrf0135319 *a Lehner *b C. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:08:22 2001 *F To: chle@uni-bayreuth.de *F Subject: Helping FlyBase: ADRC-10964 *F Dear Christian, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila Separase binds to the securin PIM and to THR, a non-securin *F protein.' *F You mention a gene that may be new to FlyBase, Sse:Separase. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If your gene does not correspond to a *F CG then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From ChLe@uni-bayreuth.de Tue Mar 06 19:52:15 2001 *F To: ''Rachel Drysdale (Genetics)'' *F Subject: AW: Helping FlyBase: ADRC-10964 *F Dear Rachel, *F The Separase gene (Sse) that is mentioned in our abstract for the upcoming *F Drosophila Conference corresponds to CG10583. *F Sincerely *F Christian Lehner # *U FBrf0135320 *a McKee *b B. *t 2001.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:00:00 2001 *F To: bdmckee@utk.edu *F Subject: Helping FlyBase: ADRC-10523 *F Dear Bruce, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of novel genes involved in meiosis in D.melanogaster.' *F You name several complementation groups 'CG this and that'. For *F reason's to do with bioinformatics we are trying to reserve gene *F symbols beginning with CG for Computed/Curated genes to do with the *F genome annotation process. I'm wondering if you would mind if we *F entered these genes into FlyBase as 'mei-CG this and that' instead, so *F they would be known in FlyBase as mei-CG1, mei-CG2, mei-CG3, mei-CG4, *F mei-CG5, mei-CG6, mei-CG7 and mei-CG8? *F Thank you for your help, *F with best wishes, *F Rachel. *F From bdmckee@utk.edu Tue Mar 06 21:02:02 2001 *F Subject: Re: Helping FlyBase: ADRC-10523 *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F We are now calling them pf-1 (pairing failure-1), pf-2, etc., because of *F their cytological phenotype. mei-pf1, mei-pf2, etc., would be ok too. *F The CG names were just stand-ins, CG meant complementation group, so I *F would not like to see them called mei-CG1, etc. ...Please let me know what you *F decide to do. *F Bruce McKee # *U FBrf0135321 *a Nakayama *b S. *t 2001.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:17:09 2001 *F To: aigaki-toshiro@c.metro-u.ac.jp *F Subject: Helping FlyBase: ADRC-10328 *F Dear Toshiro, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A novel cyclophilin-like gene required for ovulation/oviposition in *F Drosophila.' *F You mention a gene that is new to FlyBase, Cypl. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From nakashin@comp.metro-u.ac.jp Wed Mar 07 01:19:52 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Re:Helping FlyBase: ADRC-10328 *F Dear Rachel, *F I am a graduate student at Toshiro's lab. *F Cypl gene corresponds to CG13892. # *U FBrf0135322 *a Rikhy *b R. *t 2001.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:05:03 2001 *F To: ksk@tifr.res.in *F Subject: Helping FlyBase: ADRC-10076 *F Dear Dr. Krishnan, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Genetic Analysis Of Synaptic Vesicle Recycling.' *F You mention genes that are new to FlyBase, eshiA, orangi and *F vizhichai. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From richar@tifr.res.in Wed Mar 07 12:55:07 2001 *F To: K S Krishnan *F Subject: Re: Helping FlyBase: ADRC-10076 (fwd) *F Dear Rachel, *F I am replying to your mail in regard to identity of the genes mentioned in *F my abstract. *F 1. eshiA CG2210 This gene is allelic to awd. *F 2. orangi CG16944 This gene is allelic to sesB. *F 3. vizhichai 5A-6D The identity of this gene is unknown and the map *F position is as indicated. *F I hope this suffices.If there is anything else that is needed in terms of *F information then you could ask me. *F Regards, *F Richa Rikhy # *U FBrf0135323 *a Kose *b H. *t 2001.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:45:01 2001 *F To: yhiromi@lab.nig.ac.jp *F Subject: Helping FlyBase: ADRC-10583 *F Dear Yasushi, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Functional analysis of a novel protein with SAM/SPM motif, Samuel.' *F You mention a gene that is new to FlyBase, samuel. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From hkose@anex.med.tokushima-u.ac.jp Wed Mar 07 01:40:44 2001 *F Subject: Helping FlyBase: ADRC-10583 *F To: *F Dear Dr. Drysdale *F My name is Hiro Kose, a collaborator of Dr. Yasushi Hiromi at National *F Institute of Genetics, Japan. I'm writing this message in response to your *F inquiry made to Dr. Hiromi. *F Samuel gene that we are going to report at the up-coming fly meeting roughly *F corresponds to the following CGs. *F CG14918 *F CG14917 *F CG12291 *F Samuel has additional 18 amino acid residues upstream of CG14918. Also *F exon-intron boundary does not match exactly with our cDNA sequence data. *F If you have any further questions, please feel free to contact me. *F Sincerely *F Hiroyuki Kose *F \-------------------------------------------------------------------- *F Hiroyuki Kose Ph.D. *F Institute for Animal Experimentation *F The University of Tokushima School of Medicine *F 3-18-15 Kuramoto-cho, Tokushima 770-8503 Japan *F \+81-88-633-9281(Phone) *F \+81-88-633-9429(Fax) *F \-------------------------------------------------------------------- # *U FBrf0135324 *a Bhattacharya *b A. *t 2001.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:10:02 2001 *F To: bhatta@waksman.rutgers.edu *F Subject: Helping FlyBase: ADRC-10133 *F Dear Ananya, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Drosophila homolog of Nuclear Transport Factor-2 is essential for the *F anti-microbial immune response.' *F You mention a gene that is new to FlyBase, Ntf-2. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From bhatta@waksman.rutgers.edu Wed Mar 07 17:16:38 2001 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10133 *F Dear Rachael, *F This gene NTF2 does have a corresponding CG annotation. *F IT corresponds to CG1740. *F Hope this information is helpful. *F Ananya # *U FBrf0135325 *a Auld *b V. *t 2001.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:59:19 2001 *F To: auld@zoology.ubc.ca *F Subject: Helping FlyBase: ADRC-10895 *F Dear Vanessa, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A Novel Drosophila Neurexin at 94B3-4.' *F You mention a gene that is new to FlyBase, Nrx-1. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From auld@zoology.ubc.ca Wed Mar 07 17:39:15 2001 *F Subject: Re: Helping FlyBase: ADRC-10895 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel *F The CG for the neurexin that we are working on is: *F CG7050 *F which maps to the 3rd chromosome at 94B. *F Hope this helps, *F Vanessa. # *U FBrf0135326 *a Duronio *b B. *t 2001.3.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:10:22 2001 *F To: duronio@med.unc.edu *F Subject: Helping FlyBase: ADRC-10143 *F Dear Bob, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila ring finger protein dRoc1a is an essential component of an E3 *F ubiquitin ligase.' *F You mention three genes that are new to FlyBase, Roc1a, Roc1b and *F Roc2. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From duronio@med.unc.edu Wed Mar 07 19:08:37 2001 *F Subject: Re: Helping FlyBase: ADRC-10143 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F You came to the right place! I can help you out, but it will take a little *F bit of explaining because of an error that I made and need to correct in *F Genbank. *F There is a RING domain protein that is part of the SCF ubiquitin ligase. *F This protein goes by various names, including ROC1, RBX, and HRT1. As far *F as we can tell, there are three of these genes in fly, and we are calling *F them ROC1A, ROC1B, and ROC2 as per our abstract. 1A and 1B are very similar *F to each other, and to a vertebrate gene called ROC1. There is also a *F vertebrate gene called ROC2 that is most similar to a fly gene we now *F therefore call ROC2. *F Now the confusing part. Unfortunately, I submitted an entry to Genbank *F before we realized that there was three genes. We had two in hand *F (pre-genome days) that we original called ROC1 and ROC2, but that we now *F call ROC1A and ROC1B, respectively, for the reasons I stated above. *F Therefore the Genbank entry for fly ROC2 is what we are now calling ROC1B. *F We changed because there was the vertebrate ROC2 gene that is most similar *F to the new gene we found after the genome sequence came out. I will amend *F the Genbank entry, and I also put a correction note on Gadfly. *F Bottom line with respect to your question. *F CG16982 is Roc1A *F CG16988 is Roc1B, but is currently called ROC2 by Gadfly because of my *F Genbank entry that I will correct. *F ROC2 has no CG entry yet, probably because it has a huge intron in the *F middle of a tiny ORF. We think it's a real gene though. *F There is a related RING finger protein that is part of a different E3 *F ubiquitin ligase called APC11, and this is CG18042 (and the ORF is wrong in *F the annotation.) *F Sorry for the confusion. I hope this helps, and please be in touch if you *F need more information. *F Best wishes, *F Bob *F Bob Duronio *F Department of Biology *F CB#3280 *F 307 Fordham Hall *F S. Columbia Street *F University of North Carolina *F Chapel Hill, NC 27599 *F FAX: (919) 962-8472 *F VOICE: (919) 962-7749 *F EMAIL: duronio@med.unc.edu *F http://www.bio.unc.edu/faculty/duronio/ # *U FBrf0135327 *a Kassis *b J. *t 2001.3.1 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Mar 01 16:22:52 2001 *F To: jk14p@nih.gov *F Subject: Helping FlyBase: P17en1 and P17en43 *F Dear Judith, *F I have been curating this qvr paper for FlyBase *F \*x FBrf0130151 == Wang et al., 2000, J. Neurosci. 20(16): 5958--5964 *F In it the authors mention (as coming from you) 'P17en1' and *F 'P17en43' which are evidently insertions into the genome, near qvr. *F We have no record of these insertions in FlyBase. Could you tell me *F whether they have been published anywhere? If not, perhaps you could *F be specific about which P element construct is inserted in these two *F lines and where they are inserted into the genome? Then I can make *F proper insertion records for them in FlyBase. *F Many thanks for your help, *F With best wishes, *F Rachel. *F From jkassis@mail.nih.gov Thu Mar 01 20:25:00 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: P17en1 and P17en43 *F Rachel, *F Both 17en1 and 17en43 were insertions of Pen1 into chromosomal *F subdivision 48A. You can use Kassis et al., 1992, PNAS 89, 1919-1923 for a *F reference. *F Judy *F From rd120@gen.cam.ac.uk Fri Mar 02 11:54:04 2001 *F To: jkassis@mail.nih.gov *F Subject: Re: Helping FlyBase: P17en1 and P17en43 *F Thanks Judith, *F >Both 17en1 and 17en43 were insertions of Pen1 into chromosomal *F >subdivision 48A. You can use Kassis et al., 1992, PNAS 89, 1919--1923 for a *F >reference. *F 17en1 and 17en43 do not apppear to be mentioned explicitly in Kassis et *F al., 1992. We have record for P{en1}48A from that publication but *F since we have a synonym of 1-en-14 for that it seems to be a different *F insertion. We also have a record for something called P{en1}2-en-17 *F but the reference for that is *F Perrimon et al., 1991 *F Generating lineage-specific markers to study Drosophila *F development. Dev. Genet. 12: 238--252 FBrf0053745 so it is *F probably something COMPLETELY different. *F I will curate this mail from you as a personal communication to *F FlyBase, to serve as the reference for these insertions. How about *F P{en1}17-1 and P{en1}17-43 as insertion identifiers? *F Thanks again for your help. *F best wishes, *F Rachel. # *U FBrf0135328 *a Chase *b B. *t 2001.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:21:37 2001 *F To: Bruce_Chase@unomaha.edu *F Subject: Helping FlyBase: ADRC-10547 *F Dear Bruce, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila as a Model System to Study Disease Associated with Defects in *F Peroxisomal Assembly and Function.' *F You mention a gene that is new to FlyBase, Dhap-at. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From Bruce_Chase/CAS/UNO/UNEBR@unomail.unomaha.edu Thu Mar 08 20:18:21 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10547 *F Hi Rachel, *F I apologize for the delay in getting back to you. The cDNAs for *F DHAP-AT (Dihydroxyacetone phosphate acyl transferase, aka DAP-AT) we *F have correspond to CG4625. A while ago, we had submitted sequence for *F this cDNA to Genbank thinking it encoded a closely related enzyme, *F glycerol 3-phosphate acyltransferase (GPAT). This is almost certainly *F wrong, and we need to correct the Genbank submission (I will do this *F shortly). The reason we think we were wrong is that subsequent to the *F submission, we noticed that there was a targeting peptide (AKL) at the *F C-terminus of the predicted protein that directed it to the *F peroxisome. Based on this prediction of the subcellular location of *F the enzyme, and other evidence of homology with DHAP-ATs from other *F species, we now think that this transcript now encodes Drosophila *F DHAP-AT and not GPAT. We do not have direct evidence (e.g., expressing *F the full-length protein and testing its enzymatic activity). We have *F also identified (in the genome project database) another transcript *F that shows considerable sequence similarity to GPATs in other species, *F and is probably the 'real Drosophila GPAT'. We are assessing the *F enzymatic activity of the expressed protein at this time. I am *F attaching a copy of part of a simple search that might be helpful in *F clarifying what I have tried to describe above. Let me know if you *F have questions or would like additional information. I am happy to *F share it. *F Bruce *F Attached file was copy of GadFly page of CG4625 # *U FBrf0135329 *a McCaman *b R.E. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:34:54 2001 *F To: htheisen@fullerton.edu *F Subject: Helping FlyBase: ADRC-10455 *F Dear Heidi, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Gene structure and developmental regulation of the small conductance *F calcium-activated potassium channel gene (dSK) in Drosophila.' *F You mention a small conductance calcium-activated potassium channel gene, dSK. *F Do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what *F is actually the same gene unless we can't avoid it. If your gene does *F not correspond to a CG then perhaps you could tell me its map location, *F as this is valuable information for the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone better *F placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rmc89@hotmail.com Mon Mar 12 08:44:48 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10455 *F Rachel Drysdale, *F Sorry for the delay but I had to check the latest and this is a *F crushing week befor an overseas trip. So here is a detailed answer to *F your question and a background for our efforts and plans-the later are *F not fixed and we are open to suggestion. *F Rational: A few years ago, I was involved in some exploratory projects *F concerned with the detection, cloning and sequencing of transcripts of *F potential homologues of the rat Ca-activated K-channels (rsk 1-3) in *F other vertebrates (ferret, rabbit) and several invertebrates, including *F mollusks and Drosophila. Several different degenerate primer pairs *F produced PCR bands of the expected sizes in virtually all of the *F tissues explored, including Drosophila. All the ?expected? PCR bands *F were cloned and sequenced with the exception of the Drosophila PCR *F bands (due to insufficient time, resources and interest among my *F colleagues who worked with Drosophila). With the teaching *F responsibilities for an 'Introduction to Bioinformatics', the timing of *F the release of 'dripping wet' Drosophila genomic sequence and the *F potential interest of a new faculty member, Heidi Theisen, it seemed a *F propitious time to teach the students about the art of gene-finding and *F to present them with an exciting and timely opportunity to walk a *F 'virgin path'. Thus, I assigned them a project that I had some reason *F to believe would both challenge and reward them, by giving them the *F assignment to find the homologue(s) of the rsk (1-3) in the recently *F released Drosophila genome. *F We began in March of 2000 by using portions of rsk2 nucleotide sequence *F to search 'fresh' Drosophila genomic sequence through Berkley Flyblast *F and retrieving the sequences of these two unordered sequences. *F AC014402 : CSC:AC014402 . Working draft; 29275 bases *F AC014401 : CSC:AC014401 . Working draft; 11790 bases *F These clones were translate in entirety in 6-frames and peptide *F segments similar to those published for rat Ca-activated K-Channel *F (rsk2) were identified among the frames 4-6 of translation (primary *F effort of Philip Nguyen, grad student in Chemistry). These *F bioinformatics studies permitted us to order and link (or join) these *F two sequencing fragments when Celera had not. It is possible that what *F mislead Celera and their gene-finding algorithms was the sequence we *F observed of a transposable element found on the lower strand within the *F large intron that resides between the two alternatives for exon-6. Our *F 'manual' gene-finding exercises produced a hypothetical (12 exon) *F sequence (with alternatives for exon-6) that we believe encodes the *F full length of Drosophila homologues of the rsk genes. In the fall of *F 2000, wet-lab experiments in Heidi's lab (Gaby Nolazco grad student) *F involving RT-PCR of RNA extracted from Drosophila larvae, provided *F proof (through cloning and sequencing) that both of these predicted *F alternative transcripts are indeed expressed and Gaby will report on *F these findings at the Drosophila meeting. ... *F Now, in answer to your query, the most recent information made *F available on the web suggests that our transcripts actually span two *F separate predictions of Celera: *F gb|AAF46030.1| (AE003434) CG10706 gene product Drosophila melanogaster and *F gb|AAF46029.1| (AE003434) CG4179 gene product Drosophila melanogaster *F both of which are now ordered, contiguous with each other and in the *F upper strand on the X chromosome, scaffold AE003434 *F (spanning a 40kb region roughly from position's 40,000-80,000). *F regards *F Richard E. McCaman, Ph.D. *F Department of Biological Science *F P.O. Box 6850 *F Fullerton, CA 92834-6850 *F Email: rmccaman@fullerton.edu *F fax: (714) 278-3426 *F Biol. Office (714) 278-4227 *F \-------- *F Richard E. McCaman, Ph.D. *F Partner, McOno Information *F 1331 E. Nutwood Ave. *F Fullerton, CA 92831 *F voice & fax:(714)447-0964 *F efax: (209) 821-9964 # *U FBrf0135330 *a Lueders *b F. *t 2001.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:20:59 2001 *F To: Florian.Lueders@medkem.lu.se *F Subject: Helping FlyBase: ADRC-10406 *F Dear Florian, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'slalom encodes a UDP-galactose transporter important for growth factor *F signaling during patterning and morphogenesis.' *F You mention a gene that is new to FlyBase, sll. Do you know which of *F the Genome Project CG annotations your gene corresponds to? CG5802 *F perhaps? All the CGs have corresponding gene records in FlyBase already *F and we don't like to make duplicate records for what is actually the *F same gene unless we can't avoid it. If your gene does not correspond *F to a CG then perhaps you could tell me its map location, as this is *F valuable information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From florian.lueders@medkem.lu.se Fri Mar 09 15:30:38 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10406 *F Dear Rachel, *F the sll-gene corresponds to the GadFLy-annotation CG7623. *F Thanks for your mail....best wishes, *F Florian. # *U FBrf0135331 *a Harrison *b D. *t 2001.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:36:03 2001 *F To: DougH@pop.uky.edu *F Subject: Helping FlyBase: ADRC-10464, 10527, 10916 *F Dear Douglas, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstracts: *F 'Knock your SOCS off: Regulation of the JAK/STAT Pathway.' *F 'SOCS36E: a negative regulator of the JAK/STAT pathway in Drosophila *F melanogaster.' *F 'Biochemical interactions between the regulatory factors of the JAK/STAT *F pathway.' *F You mention three Socs genes, Socs36E, Socs16E and Socs44E. Do you *F know which of the Genome Project CG annotations your genes correspond *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If any of your genes does not correspond *F to a CG then perhaps you could tell me its map location, as this is *F valuable information for the genome annotation project. *F Also, we already have a gene record for something called Socs, *F published by you in last year's abstracts, which maps to clone *F DS04489. Does this correspond to Socs36E, or one of the others? *F Thank you for your help, *F with best wishes, *F Rachel. *F From DougH@pop.uky.edu Fri Mar 09 17:07:54 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10464, 10527, 10916 *F From: Doug Harrison *F Hi Rachel, *F Each of the Socs genes referenced in the abstracts does correspond *F to a CG record, although our cDNA and EST clones appear to have some *F differences from the predicted gene structure (but we will provide more on *F that over the next few months as we complete sequencing). Below are the *F relationships of the predicted Socs genes to the predicted CG gene *F records. As you surmised, our reference to Socs from last year's abstract *F refers to Socs36E. If you have further questions, feel free to let me *F know. We will provide you with updated information about gene structure as *F we confirm it. *F Socs36E = CG15154 \+ some additional sequence that encodes a Socs box *F Socs16D = CG8146 \+ Socs box sequence *F Socs44A = CG2160 *F Doug # *U FBrf0135332 *a Samakovlis *b C. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:35:34 2001 *F To: christos@ucmp.umu.se *F Subject: Helping FlyBase: ADRC-10458 *F Dear Christos, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Selective requirement of nucleoporins in immune response activation.' *F You mention a gene that is new to FlyBase, Nup214. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG then *F perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From christos@ucmp.umu.se Mon Mar 12 13:47:41 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10458 *F Dear Rachel *F CG3820 is the homolog of the human Nucleoporin 214 also known as CAN214 *F Christos *F Christos Samakovlis *F Umeå Center for Molecular Pathogenesis UCMP *F Umeå University Building 6L *F S-90187 Umeå *F Sweden *F Fax: 46-90-778007 *F Tel: 46-90-7856785 # *U FBrf0135333 *a Gindhart *b J. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:37:36 2001 *F To: joseph.gindhart@umb.edu *F Subject: Helping FlyBase: ADRC-10489 *F Dear Joseph, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The kinesin-associated protein Dunc-76 is required for axonal transport in *F the larval nervous system.' *F You mention a gene that is new to FlyBase, Unc-76. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From joseph.gindhart@umb.edu Mon Mar 12 14:30:36 2001 *F To: 'Rachel Drysdale (Genetics)' , *F Subject: Re: Helping FlyBase: ADRC-10489 *F Dear Rachel, *F Drosophila unc-76 (Dunc-76 or Unc-76, if you prefer I call it that) *F corresponds to CG3981. It is just distal to csw (the 3'end of *F Dunc-76 is about 1kb 5' of the csw transcription start site. *F Interestingly, in the first genome annotation CG3981 had a large *F (about 14kb) intron. In the second version of Gadfly, however, this *F intron is shown as intergenic space, and CG3981 is split into two *F genes. We think that the first version (one big gene, not two small *F genes) is correct. Let me know if you have any questions. *F Best Regards, *F Joe *F Rachel Drysdale (Genetics) wrote: # *U FBrf0135334 *a Mattox *b W. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:57:32 2001 *F To: wmattox@notes.mdacc.tmc.edu *F Subject: Helping FlyBase: ADRC-10865 *F Dear Bill, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of three doublesex related genes suggests that they play roles in *F sexual differentiation.' *F You mention three genes that are new to FlyBase, dmrt99B, dmrt93B and *F dmrt11E. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From wmattox@mail.mdanderson.org Mon Mar 12 14:31:40 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10865 *F Rachel, *F Here is the info you asked for on the dmrt genes. *F dmrt11E = CG15749 *F dmrt93B = CG5737 *F dmrt99B =CG15504 *F Bill # *U FBrf0135335 *a Hazelrigg *b T. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:54:52 2001 *F To: tih1@columbia.edu *F Subject: Helping FlyBase: ADRC-10835 *F Dear Tulle, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Functional analysis of the Eap gene during Drosophila oogenesis.' *F You mention a gene that is new to FlyBase, Eap. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From tulle@cubsps.bio.columbia.edu Mon Mar 12 16:59:45 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10835 *F Hi Rachel, *F Eap is CG3594. *F Hope this helps! *F Best wishes, *F Tulle # *U FBrf0135336 *a Shi *b W. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:51:08 2001 *F To: jskeath@genetics.wustl.edu *F Subject: Helping FlyBase: ADRC-10735 *F Dear Jim, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of side-out, a novel Drosophila gene involved in embryonic *F lateral CNS development.' *F You mention a gene that is new to FlyBase, side-out. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. Also you suggest the *F abbreviation sdt for side-out, but sdt is already in use to denote the *F stardust locus (FBgn0003349), so I am afraid you will have to choose *F another symbol. At time of writing sid is available, or I am happy to *F check out any other suggestions that you might like to make. *F Thank you for your help, *F with best wishes, *F Rachel. *F From wshi@artsci.wustl.edu Mon Mar 12 18:32:14 2001 *F To: Rachel Drysdale *F Subject: Re:Helping FlyBase: ADRC-10735 *F Dear Rachel, *F I am really sorry for the late response. I have been working on *F my meeting poster lately and somehow forgot the message. So, *F recently we have obtained several EMS alleles of the gene and it *F turned out to be a previously identified gene, Bj1 (CG10480), the *F Drosophila homolog of vertebrate RCC1 gene. It is still not clear *F why this seemingly ubiquitously expressed gene causes the more *F restricted phenotype in the lateral CNS, but we are currently *F working on it. *F Sorry for the previous confusion and we do not need any new names *F now:) *F Thanks again for your correspondence. *F Sincerely, *F Weiyang Shi # *U FBrf0135337 *a King-Jones *b K. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:51:59 2001 *F To: kirst@howard.genetics.utah.edu *F Subject: Helping FlyBase: ADRC-10766 *F Dear Kirst, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Regulation and function of DHR4 and DHR96, two members of the nuclear *F receptor superfamily, during metamorphosis.' *F You mention the DHR4 and DHR96 genes. We have a record for Hr96 *F (FBgn0015240) but not for DHR4, though it is not entirely clear to me *F that DHR4 is newly discovered. Do you know which of the Genome Project *F CG annotations DHR4 corresponds to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F If your gene does not correspond to a CG then perhaps you could tell me *F its map location, as this is valuable information for the genome *F annotation project. If DHR4 is a new gene I will make a record for it *F though will have to drop the D for Drosophila in the valid symbol and *F would follow the lead of Hr96 and call it Hr4. *F Thank you for your help, *F with best wishes, *F Rachel. *F From kirst@howard.genetics.utah.edu Mon Mar 12 18:39:46 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10766 *F Hi Rachel, *F DHR4 corresponds to CG16902 (EG:133E12.2). *F see you, *F Kirst # *U FBrf0135338 *a Farkas *b R. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:43:21 2001 *F To: rfarkas@leland.stanford.edu *F Subject: Helping FlyBase: ADRC-10572 *F Dear Rebecca, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of mechanisms common to cytokinesis and polarized cell growth in the *F Drosophila male germline.' *F You mention two genes that are new to FlyBase, onr and bns, and also *F fws. Do you know which of the Genome Project CG annotations these *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to a CG then perhaps you could tell me their map locations, *F as this is valuable information for the genome annotation project. *F Also, if possible we would like to record the full name of onr and bns *F (presumably abbreviations), so perhaps you could let me know the full *F names too. *F Thank you for your help, *F with best wishes, *F Rachel. *F From rfarkas@stanford.edu Mon Mar 12 22:03:03 2001 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10572 *F Dear Rachel, *F ..... *F We *F are currently writing up a paper on a cytokinesis screen, and we would *F like to wait until it is published before providing information on most of *F the genes to Flybase. However, I am happy to give you more information on *F four way stop (fws). It maps to 36C and corresponds to CG6549. I have two *F alleles, both are viable/male sterile. They were generated by EMS. I have *F not yet checked for female sterility. *F ... *F Best wishes, *F Rebecca Farkas *F Developmental Biology Dept. *F B300 Beckman Center *F 279 Campus Drive *F Stanford, CA 94305 Phone: 650-723-5285 Fax: 650-725-7739 # *U FBrf0135339 *a Parker *b L. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:42:14 2001 *F To: karora@uci.edu *F Subject: Helping FlyBase: ADRC-10562 *F Dear Kavita, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'ALP23B, a novel member of the TGF-beta superfamily.' *F You mention a gene that is new to FlyBase, Alp23B. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From parkerj@uci.edu Mon Mar 12 22:34:08 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Re. Helping FlyBase: ADRC-10562 *F Hi Rachel, *F You sent Kavita an email about my poster on ALP23B for the conference. *F The gene corresponds to CG16987, however, ALP23B is only a temporary *F name until I can come up with something better based on phenotype, *F theres already a gene called Alp so I'd have to change it anyway . My *F email is listed with Flybase although I updated recently and it may not *F have been changed yet. *F Yours sincerely, *F Louise Parker # *U FBrf0135340 *a O'Brien *b N. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:06:19 2001 *F To: nwdworth@sunstroke.sdsu.edu *F Subject: Helping FlyBase: ADRC-10089 *F Dear Nicole, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Cloning and characterization of a novel actin binding protein in Drosophila *F melanogaster.' *F You mention a gene that is new to FlyBase, Abp. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From nobrien@sunstroke.sdsu.edu Mon Mar 12 23:25:27 2001 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10089 *F Hi Rachel, *F dabp corresponds to CG8953, which right now is named Actn3 by FlyBase.. *F However, based on sequence homology, only the actin binding domain is *F homologous to human alpha-actinin 3 and while the rest is something *F else. As well, this putative actin-binding domain is at the C terminal *F end rather than the N terminal end as it is on other alpha-actinins, so *F I am proposing that this gene is not alpha actinin 3 and I am calling it *F drosophila actin binding protein. However, I have not yet demonstrated *F that it unequivocally binds actin as of yet, so my name is not *F necessarily fit for this gene either. Hope that helps. *F Nicole O'Brien # *U FBrf0135341 *a Steneberg *b P. *t 2001.3.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Thu Mar 01 16:16:40 2001 *F To: christos@ucmp.umu.se *F Subject: Helping FlyBase: Fus-6 *F Dear Christos, *F I am writing to you about a/two enhancer trap insertion(s) in FlyBase. *F We have two distinct records at the moment, for *F FBti0005754 ==== P{lacW}B9-3-52 *F FBti0010411 ==== P{lacZ}hdcFus-6 *F The question is, are these the same thing or not? *F The relevent references are: *F FBrf0122647 ==== Jan, 1990.8.1, personal communication to FlyBase *F FBrf0057603 ==== Hartenstein and Jan, 1992, Roux Arch. dev. Biol. 201(4): 1= *F 94--220 *F FBrf0090781 ==== Samakovlis et al., 1996, Development 122(11): 3531--3536 *F FBrf0101899 ==== Steneberg et al., 1998, Genes Dev. 12(7): 956--967 *F FBrf0101899 states unambiguously (page 965, 3rd last line) that 'The *F Fus-6, B9-3-52 enhancer trap allele was generated in the lab of Y.N. *F Jan' and references FBrf0057603. *F FBrf0057603 does not address the viability of B9-3-52 *F FBrf0122647 says B9-3-52 is viable *F FBrf0101899 says Fus-6 is lethal *F FBrf0057603 doesn't mention trachea though says they looked at stages 16/17 *F FBrf0101899 sees tracheal expression at stage 14 *F FBrf0090781 suggests tracheal expression at stage 15 *F FBrf0101899 says Fus-6 is at 99F *F FBrf0090781 says Fus-6 is at 99D *F help! *F we are not sure how best to resolve this confusion \- I think you are *F the best person to help us out. Can you throw any light on this for *F us? We would be most grateful for any help you can give. *F With best regards, *F Rachel. *F From Per.Steneberg@ucmp.umu.se Mon Mar 12 14:23:15 2001 *F Subject: Helping FlyBase: Fus-6 *F Dear Rachel, *F I'm answering in Christos place on the message you sent the 1rst of March. *F P(lacW)B9-3-52 and P(lacZ)hdc(Fus-6) is the same strain and it is a lethal *F P-element insertion. *F Ref. FBrf0090781 \- tracheal expression done by stainings towards *F b-Galactosidase, which was detected from stage 15 *F Ref. FBrf0101899 \- tracheal expression done by stainings towards the Hdc *F protein with a monoclonal Hdc antibody, which was detected from stage 14. *F Fusion-6 is located at position 99F on the third chromosome. *F Best regards, *F Per Steneberg # *U FBrf0135342 *a Hou *b S. *t 2001.3.13 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:20:00 2001 *F To: shou@mail.ncifcrf.gov *F Subject: Helping FlyBase: ADRC-10381 *F Dear Steven, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'CKA, a novel multidomain protein, regulates the JNK signal transduction *F pathway in Drosophila.' *F You mention a gene that is new to FlyBase, Cka. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From shou@mail.ncifcrf.gov Tue Mar 13 15:44:53 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10381 *F Our gene mapped 28E. I don't know which GC gene corresponds to it. *F Steven Hou, Ph.D # *U FBrf0135343 *a Frolov *b M. *t 2001.3.13 *T personal communication to FlyBase *u *F From rd120 Mon Mar 5 11:37:57 2001 *F To: frolov@helix.mgh.harvard.edu *F Subject: Helping FlyBase: ADRC-10490 *F Dear Maxim, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Function of de2f2 in development.' *F You mention a gene that is new to FlyBase, Mpp6. Your abstract states that *F this gene is adjacent to E2f2. The E2f2 neighbours are all CG genes, but *F CG9250 has homology to 'pMPP-6| M-phase phosphoprotein 6(aa). Does Mpp6 *F correspond to CG9250? *F Thank you for your help, *F with best wishes, *F Rachel. *F From frolov@helix.mgh.harvard.edu Tue Mar 13 20:38:27 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10490 *F Dear Rachel, *F Yes, mpp6 corresponds to CG9250. Should we refer to this gene by the CG *F number in the future? *F Thank you. *F Sincerely *F Max. # *U FBrf0135344 *a Andrew *b D. *t 2001.3.13 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:41:21 2001 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 5 Mar 2001 11:41:21 \+0000 *F To: dandrew@jhmi.edu *F Subject: Helping FlyBase: ADRC-10549 *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 5 Mar 2001 11:41:20 \+0000 *F Content-Length: 1182 *F Dear Deborah, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Discovery and Function of the Gene serrano in Drosophila.' *F You mention a gene that is new to FlyBase, sano. Do you know which of *F the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From dandrew@bs.jhmi.edu Tue Mar 13 21:26:30 2001 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 13 Mar 2001 21:26:30 \+0000 *F Date: Wed, 14 Mar 2001 04:25:02 \-0400 *F From: Debbie Andrew *F Subject: RE: Helping FlyBase: ADRC-10549 *F To: Rachel Drysdale *F Reply-to: Debbie Andrew *F MIME-version: 1.0 *F X-Mailer: QuickMail Pro 1.5.3b3 (Mac) *F Content-transfer-encoding: 7bit *F X-Priority: 3 *F Reply to: RE: Helping FlyBase: ADRC-10549 *F Hi Rachel, *F The Celera Genomics sequence for serrano (sano) is CG12758. *F Debbie # *U FBrf0135345 *a Zurovec *b M. *t 2001.3.14 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:40:47 2001 *F To: pjbryant@uci.edu *F Subject: Helping FlyBase: ADRC-10546 *F Dear Peter, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'ADGFs \- growth factors with enzymatic activity.' *F You mention two genes that are new to FlyBase, Adgf-A and Adgf-D. Do *F you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to a CG then perhaps you could tell me their map locations, *F as this is valuable information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From mzurovec@uci.edu Wed Mar 14 04:05:59 2001 *F To: pjbryant@uci.edu *F Cc: 'Rachel Drysdale (Genetics)' *F Subject: ADGF genes *F Dear Rachel: *F I am sorry for the delay in answering your letter. The Genome project *F annotations of ADGF genes are: *F ADGF-A (CG5992), *F ADGF-D (CG9621) *F Best regards *F Michal Zurovec # *U FBrf0135346 *a Lin *b X. *t 2001.3.14 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:58:25 2001 *F To: linyby@chmcc.org *F Subject: Helping FlyBase: ADRC-10879 *F Dear Xinhua, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of botv, a new segment polarity gene that encodes a *F protein of the putative tumor suppressor EXT family.' *F You mention a gene that is new to FlyBase, botv. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From linyby@chmcc.org Wed Mar 14 06:28:28 2001 *F To: *F Subject: Re: Helping FlyBase: ADRC-10879 *F Dear Rachel: *F ... The gene botv is CG15110 or BcDNA: LD21192. Please let me know *F if there is other info needed. *F Xinhua Lin # *U FBrf0135347 *a Roberts *b I. *t 2001.3.14 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:18:20 2001 *F To: i.j.h.roberts@reading.ac.uk *F Subject: Helping FlyBase: ADRC-10338 *F Dear Ian, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of G-protein beta and gamma subunit signalling using Drosophila.' *F You mention a gene that is new to FlyBase, G&bgr;5. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you for your help, *F with best wishes, *F Rachel. *F From i.j.h.roberts@reading.ac.uk Wed Mar 14 10:15:50 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10338 *F Rachel, *F This gene corresponds to CG10763. *F Thanks for all your good work and help. *F Ian. *F Dr. Ian JH Roberts *F School of Animal & Microbial Sciences *F University of Reading *F Whiteknights, P.O. Box 228 *F Reading RG6 6AJ *F England. *F Phone \+44 (0)118 987 5123 Ext.7074 *F Fax \+44 (0)118 931 0180 *F Email: i.j.h.roberts@reading.ac.uk *F http://www.ams.rdg.ac.uk/zoology/roberts/ # *U FBrf0135348 *a Carpenter *b A.T.C. *t 2001.3.12 *T personal communication to FlyBase *u *F From atc12@mole.bio.cam.ac.uk Mon Mar 12 23:22:05 2001 *F To: Aubrey de Grey *F Subject: ?merger of Cy3-23 and nuf? *F Cy3-23/nuf1 females lay abundant eggs none of which hatch (and most die *F unpigmented); the addition of P{Germ10-nuf} to such females does permit *F some egg hatch => normal larvae though possibly fewer than occur in G10/+; *F nuf1/nuf1 females. Both mutant alleles fail to complement Df(3L)D5 *F (70D2-4) (me also). All this points to allelism.... *F Adelaide # *U FBrf0135349 *a Hummel *b T. *t 2001.3.14 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 12:04:44 2001 *F To: zipursky@hhmi.ucla.edu *F Subject: Helping FlyBase: ADRC-10955 *F Dear Larry, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Premature glial migration disrupts axon pathfinding in the Drosophila *F visual system.' *F You mention a gene that is new to FlyBase, gish. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From hummel@hhmi.ucla.edu Wed Mar 14 17:12:29 2001 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10955 *F Dear Rachel, *F Larry forwarded your question to me about the location of gilgamesh (gish). *F gish encodes a casein kinase I gamma homologe (89B17-19), which corresponds *F to CG6963. *F Best wishes, *F Thomas # *U FBrf0135350 *a Voas *b M. *t 2001.3.14 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:57:00 2001 *F To: voas@wi.mit.edu *F Subject: Helping FlyBase: ADRC-10863 *F Dear Matthew, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of a potential negative regulator of EGFR/Ras/MAPK signalling.' *F You mention a gene that may be new to FlyBase ('a novel gene that may *F function as a negative regulator of the EGFR/Ras/MAPK signalling *F pathway'), but do not name it. Do you have a name/symbol by which you *F refer to this mutant. Do you know which of the Genome Project CG *F annotations your gene corresponds to? Your abstract suggests you know *F enough about the molecular biology of the gene that this link can be *F made. All the CGs have corresponding gene records in FlyBase already *F and we don't like to make duplicate records for what is actually the *F same gene unless we can't avoid it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From voas@wi.mit.edu Wed Mar 14 21:34:22 2001 *F Subject: RE: Helping FlyBase: ADRC-10863 *F To: 'Genetics' *F Dear Rachel, *F Sorry for my late response. My gene corresponds to CG7036. I have not yet *F named it though. *F Hope this info does not reach you too late. *F Best regards, *F Matt Voas # *U FBrf0135351 *a Lehmann *b R. *t 2001.3.15 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:44:45 2001 *F To: lehmann@saturn.med.nyu.edu *F Subject: Helping FlyBase: ADRC-10577 *F Dear Ruth, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization and cloning of two genes controlling germ cell *F migration: Where's waldo and what's 9.35?' *F You mention a gene that is new to FlyBase, 9.35. Do you know which of *F the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. Similarly, it sounds *F like you might have defined waldo by now, and if you could let me know *F which CG it is/where it is then that would be similarly helpful. *F I am writing to you because the first author does not have an address *F in FlyBase \- please feel free to pass my query on if there is someone *F better placed to answer it. *F Thank you for your help, *F with best wishes, *F Rachel. *F From lehmann@saturn.med.nyu.edu Thu Mar 15 21:42:05 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10577 *F Dear Rachel, *F we have mapped waldo to 26D1,2 and 9.35 is not further mapped except that *F it is on 2L between dp and b. # *U FBrf0135352 *a McBride *b E. *t 2001.3.16 *T personal communication to FlyBase *u *F >From rd120@gen.cam.ac.uk Mon Mar 05 12:09:16 2001 *F To: EMcBride@lifespan.org *F Subject: Helping FlyBase: ADRC-10113 *F Dear Edward, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Molecular Cloning, Genomic Organization and Expression of a Drosophila *F Cholecystokinin-like Receptor.' *F You mention two genes that are new to FlyBase, though do not name *F them. Do you know which of the Genome Project CG annotations your *F genes correspond to? Might they be CG6894 and CG6857? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your genes do not correspond to CGs then perhaps *F you could tell me their map location, as this is valuable information *F for the genome annotation project. Also, this would be a good time for *F you to suggest a gene name each for your new genes (CckR-17D1 and *F CckR-17D3 perhaps?). *F Thank you for your help, *F with best wishes, *F Rachel. *F From EMcbride@lifespan.org Fri Mar 16 13:05:23 2001 *F To: 'Rachel Drysdale ' *F Subject: RE: Helping FlyBase: ADRC-10113 *F Rachel, *F .....The genes do correspond to CG6857 and a combination of *F CG6894 and adjacent CG6881. We've been holding off on naming the genes *F pending completion of functional analysis. Up to this point, I've been *F referring to them using the CG annotation. If names are required now, those *F that you suggested would be fine perhaps with the slight change CCKLR-17D1 *F and CCKLR-17D3 (indicating that they are CCK-like receptors to reflect that *F we've been unable to establish that Drosophila express a CCK-related *F peptide). *F ... *F Ed McBride # *U FBrf0135353 *a Hall *b L. *t 2001.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 05 11:53:41 2001 *F To: kweyu@ucdavis.edu *F Subject: Helping FlyBase: ADRC-10803 *F Dear Kweon, *F We are currently curating the abstracts for the upcoming 42nd *F (Washington, D.C.) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Cloning and Functional Characterization of a Neuropeptide F-like Receptor.' *F You mention a gene that is new to FlyBase, at 76D5--76F2. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. Also, you don't name *F your gene at all. Are you in a position to give a proper name to this *F gene now. *F Thank you for your help, *F with best wishes, *F Rachel. *F >From linhall@ucdavis.edu Mon Mar 19 23:52:15 2001 *F To: rd120@gen.cam.ac.uk *F Subject: HelpingFlyBase:ADRC-10803 *F you wrote to kweon yu asking about the abstract entitled: 'Cloning and *F Functional Characterization of a Neiripeptide F-like Receptor' at position *F 76D5-76F2. *F this is a gene we have been working on for several years...predating the *F genome project. it was originally cloned by reduced stringency pcr looking *F for other members of the g protein coupled receptor family. people *F involved in the original cloning were gouping feng, renee venard, and me *F (linda m. hall) all working in my lab. subsequent pharmacology and *F expression characterization have involved peter evans (from cambridge, uk) *F and kweon yu working in my lab here in sacramento. *F in the genoome project, thos gene corresponds to CG7395. from its *F structure we deduced that it was a peptide receptor, pharmacological *F studies involving expression in xenopus oocytes shows that it best *F activated by a neuropeptide F-like peptide. based on its pharmacology and *F structure we propose to call it NPFR76F for neuropeptide F-like receptor *F located at 76F. *F we are finalizing a manuscript on this now. *F let me know if you need additional information. *F cheers, *F Linda M. Hall, Ph.D. *F Director of Research *F Shriners Hospital for Children of Northern California *F 2425 Stockton Boulevard *F Sacramento, CA 95817 *F Cell phone 916-208-2465 (on most of the time) *F Phone (Office) 916-734-6590; Phone (Lab) 916-734-6593 *F Fax 916-734-6591 # *U FBrf0135354 *a Ingham *b P. *t 2001.3.29 *T personal communication to FlyBase *u *F From p.w.ingham@sheffield.ac.uk Thu Mar 29 14:44:12 2001 *F To: flybase-update@morgan.harvard.edu *F Subject: new patched alleles *F We have recently isolated 16 new patched alleles which we have designated *F ptc32 through ptc47. Please can you add these to the database as soon as *F possible since the majority of these are about to be described in a paper *F entitled: 'Mutations in the Sterol Sensing Domain of Patched suggest a Role *F for Vesicular Trafficking in Smoothened Regulation' by H. Strutt, C. *F Thomas, Y. Nakano, D. Stark, B. Neave, A.M Taylor & P. W. Ingham that is in *F press in Current Biology. *F Many thanks, *F Yours sincerely, *F Philip Ingham, *F MRC Intercellular Signalling Group, *F Centre for Developmental Genetics, *F Department of Biomedical Science, *F University of Sheffield, *F Firth Court, *F Western Bank, *F Sheffield S10 2TN *F U.K. *F Tel: 44 (0)114 222 2710 (Personal Assistant) *F Fax: 44 (0)114 222 2788 (Direct) *F http://www.shef.ac.uk/uni/academic/A-C/biomsc/research/ingham.html # *U FBrf0135355 *a Hassan *b B. *t 2001.3.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Mar 30 23:47:51 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-GAL4.H} insertions *F The following information accompanied stocks from Bassem Hassan, Baylor *F College of Medicine (3/01). *F P{UAS-GAL4.H}24 is a homozygous and hemizygous viable and fertile X *F chromosome insertion. *F P{UAS-GAL4.H}12B is a homozygous viable and fertile second chromosome *F insertion. *F P{UAS-GAL4.H}3A is a homozygous viable and fertile third chromosome insertion. *F The construct and its transformation were described in Hassan et al., 2000, *F Neuron 25(3): 549-561 FBrf0127108. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0135356 *a Bulgakova *b N. *c S. *d Trunova *e L. *f Omelyanchuk *t 2001.3.23 *T personal communication to FlyBase *u *F From nbul@bionet.nsc.ru Fri Mar 23 13:06:24 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: New Indy allele *F IDENTIFICATION OF NEW Indyp115 ALLELE. *F Bulgakova N., Trunova S., Omelyanchuk L. *F Mutation Indyp115, caused by PlArB insertion in 75D region, was *F initially isolated as cell-cycle related. Rescued genomic DNA was *F cloned and sequenced. *F Molecular data: P-target rescue placed this insertion of *F PlArB-element in forward direction within 122 bp of putative *F transcriptional start site. We found that EP(3)3044 is localized in *F reversed orientation here. *F Mutant phenotype: Homozygotes are fertile but have decreased viability. *F The reporter b-galactosidase activity was detected in larval brain *F (Fig1), eye-antennae imaginal disk (the staining was observed after the *F front of morphogenetic furrow, Fig2), larval midgut (imaginal ring and *F all the cells of the gut excluding gut brunches, Fig3), fat body *F (Fig4). In adult flies staining was revealed in oenocytes, testis *F (Fig5), and ovaries (region 2 in germaria, Fig6, some nurse cells, *F stripped staining of the follicular cells in 14th stage old egg *F chambers, Fig7). The determination of the lethal phase in *F embryogenesis shows one lethal phase at 1-5 embryonic stages and a *F second at 15-16 stages. Since the first lethal phase takes phase before *F blastoderm stage where no zygotic genes are active we conclude that *F p115 shows maternal effect on the viability of progeny. We suggest that *F the early embryonic lethality may be due to wrong gene expression *F detected by b-galactosidase staining in follicular cells. *F Other information: Initially this mutation was described and studied as *F male sterile (Kozlova A.V., Omelyanchuk L.V., Y-chromosomal factor *F controls transctiption of autosomal fertility genes in Drosophila *F melanogaster males. Ontogenez 1998 29(5): 366-372). The excision of *F PlArB-element shows that the sterile phenotype is not connected with *F insertion.With the use of meiotic crossingover we localized the male *F sterile mutation in the ru \- h interval (61F7-66D10). *F Bulgakova Natalia A. *F Master Degree Student *F Laboratory of Cell Cycle Genetics *F Institute of Cytology and Genetics *F Russian Academy of Sciences *F Lavrentiev avenue, 10 *F Novosibirsk 630090 *F Russia *F nbul@bionet.nsc.ru # *U FBrf0135360 *a Marsh *b L. *t 2001.4.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Apr 03 15:35:54 2001 *F To: jlmarsh@uci.edu *F Subject: Helping FlyBase: tsg2 *F Dear Larry, *F I am looking at your paper in Nature (Vol 480:479--482 'Twisted *F gastrulation is a conserved ...') curating it for our Genes file and *F note that you mention a second tsg gene called tsg2, of which we have *F no record with that name. However it is likely that you know which CG *F it corresponds to, and we do have gene records for all the CG genes. *F To avoid us creating a second gene record (with the tsg2 symbol) for a *F gene which we already have a record for (with a CG symbol) please could *F you tell me which CG it is? *F With best wishes, *F Rachel. *F From jlmarsh@uci.edu Tue Apr 10 17:32:58 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: tsg2 *F Dear Rachel, *F Yes. The cg is cg12410... *F Larry Marsh *F J.Lawrence Marsh, Ph.D. *F Professor *F Dept. Developmental and Cell Biology *F University of California Irvine *F Irvine, CA 92697-2300 *F Voice: (949) 824-6677 *F FAX: (949) 824-3571 *F email: JLMarsh@UCI.edu *F http://darwin.bio.uci.edu/~marshlab/ # *U FBrf0135361 *a Matthews *b K. *t 2001.4.12 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Fri Apr 13 00:10:04 2001 *F Subject: bel alleles *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Kathy Matthews, Indiana University *F Subject: bel alleles *F Dated: 12 April 2001 *F Information communicated: *F belM2 *F EMS-induced on kniri-1 e1, selected for lethality with bel6. *F Fails to complement Df(3R)p13. belM2/Df(3R)Antp17, *F alphaTub67CRE1.1 heterozygotes, which have half the normal *F complement of wild-type maternally-expressed alpha-tubulin *F genes, are semi-sterile. *F belM3 *F EMS-induced on kniri-1 e1, selected for lethality with bel6. *F Fails to complement Df(3R)p13. belM3/Df(3R)Antp17, *F alphaTub67CRE1.1 heterozygotes, which have half the normal *F complement of wild-type maternally-expressed alpha-tubulin *F genes, are semi-sterile. *F belM4 *F EMS-induced on kniri-1 e1, selected for lethality with bel6. *F Fails to complement Df(3R)p13. Fertility of *F belM4/Df(3R)Antp17, alphaTub67CRE1.1 heterozygotes, *F which have half the normal complement of wild-type maternally- *F expressed alpha-tubulin genes, is good relative to that of *F comparable heterozygotes with belM2 and belM3. # *U FBrf0135362 *a Treisman *b J. *t 2001.4.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 17 17:34:43 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Treisman P insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jessica Treisman, New York University Medical Center (4/01). *F 1. P{UAS-capt.B}1 FBti0016171 is a homozygous viable and fertile *F insertion on the second chromosome. *F 2. P{UAS-osa}s2 FBti0015812 is a second chromosome insertion. The *F insertion-bearing chromosome is homozygous lethal. *F 3. P{UAS-msn.S}2 new insertion identifier is a homozygous viable and *F fertile insertion on the second chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0135387 *a Rabinow *b L. *c L. *d Rabinow *t 2001.5.23 *T personal communication to FlyBase *u *F The following information was communicated by Leonard Rabinow on 5/23/2001 *F in response to a curator query: *F "We are not sure about the reality of the longer of the two Doa isoforms *F described in Flybase/BDGP (exon 1, 580 AA protein), since our westerns *F don't reproducibly show a protein of that size. We cannot yet rule *F that one out however. We do see a protein of ca. 56 kD which we believe is *F the 511 AA protein (starts exon 2). *F *F The 105 kD DOA protein is incompletely characterized, but the new class of *F cDNAs we have are probably those responsible for it (see: Yun et al. *F Genetics 2000 156: 749-761). There are no ESTs in BDGP which correspond to *F them, at least to date (5-01). What is very surprising is that the 4 new *F exons so-far characterized encoding this protein (and we've not yet found *F the 5' end) are spread out over about an additional 70 kB of 3R, and are *F interspersed among numerous other genes on both strands. Based on the fact *F that this alternative cDNA splices into the fully characterized Doa mRNA *F sequence exactly at a known splice junction just upstream of the catalytic *F domain, plus some other data, we're quite sure these alternative exons are *F real." # *U FBrf0135638 *a Hoskins *b R. *t 2001.5.3 *T personal communication to FlyBase *u *F >From hoskins@currant.lbl.gov Thu May 03 18:12:10 2001 *F Envelope-to: m.ashburner@gen.cam.ac.uk *F Delivery-date: Thu, 3 May 2001 18:12:10 +0100 *F Date: Thu, 03 May 2001 10:12:14 -0700 *F From: 'Roger A. Hoskins' *F Organization: Lawrence Berkeley National Laboratory *F X-Mailer: Mozilla 4.75C-CCK-MCD {C-UDP; EBM-APPLE} (Macintosh; U; PPC) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: m.ashburner@gen.cam.ac.uk *F Subject: strain nomenclature for isogenics *F Content-Transfer-Encoding: 7bit *F *F Michael, *F *F I'm sending the strains used in our SNP mapping to Bloomington. The *F strains are derived from Canton S and Oregon R from Gerry's lab. They *F were passed over balancers to isogenize each of the major chromosomes in *F separate lines. I've been calling the six wild-type strains CSisoX, *F CSiso2, CSiso3, ORisoX, ORiso2 and ORiso3. I'd prefer to follow standard *F fly nomenclature. Is there a standard for naming this kind of strain? *F *F Thanks, *F *F Roger # *U FBrf0135639 *a Butler *b M. *t 2001.4.30 *T personal communication to FlyBase *u *F >From margi@sanger.otago.ac.nz Mon Apr 30 06:14:43 2001 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 30 Apr 2001 06:14:43 +0100 *F Date: Mon, 30 Apr 2001 17:14:38 +1200 (NZST) *F From: Margi Butler *F Reply-To: Margi Butler *F To: ma11@gen.cam.ac.uk *F Subject: Tinker (a BEL-like element) *F MIME-Version: 1.0 *F *F *F Dear Professor Ashburner *F *F Dr Poulter has asked me to reply to your e-mail about the Drosophila *F retrotransposon 'Tinker' on AC004377. *F *F The coordinates of the LTR's: left LTR 77835-77612 *F right LTR 71724-71947 *F *F The ORF co-ordinates (the complement) : ATG (start) 77259 *F TAA (stop) 72016 *F (5244bp, 1748aa) *F *F As Mr Frame is no longer in the lab., I hope I have extracted the data *F correctly! I can't find a good reason to believe that one LTR is 21 bases *F longer than the other...(as mentioned in the paper). If I do, after *F discussion with either Ian or other lab members, I will let you know. *F Please accept my apologies for the hurried reply, thank you very much for *F your interest. *F Yours sincerely *F *F Margi # *U FBrf0135967 *a Bourouis *b M. *t 1999.6.7 *T personal communication to FlyBase *u *F Archived. # *U FBrf0135974 *a Gelbart *b W.M. *t 2001.4.19 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed Apr 18 18:18:39 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 18 Apr 2001 18:18:39 \+0100 *F To: gelbart@morgan.harvard.edu *F Subject: help with a chromosome *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 18 Apr 2001 18:18:40 \+0100 *F Content-Length: 775 *F Hi Bill, *F in a Genetics paper I am curating: *F Su et al., 2001, Genetics 157(2): 717--725 *F they use a chromosome which comes from your lab. I wrote to Stuart *F Newfeld about it and he said I should ask you. *F The chromosome in question is called 'Df(2L)DTD51xD52' in the paper and *F is in Figure 2 of the paper. From that figure it looks like it takes *F out 23A and 23B. *F (in the paper they say it is described in FBrf0038632 == Gelbart, 1982, *F Proc. Natl. Acad. Sci. USA 79(8): 2636--2640, but I looked and it *F isn't) *F We don't have this chromosome in FB but before I go and make a new *F deficiency record for it I wanted to check that it isn't some funky *F artificial deletion that is made up of two overlapping translocation *F segregants and is just an ordinary deficiency. *F ta, *F Gillian *F From gelbart@morgan.harvard.edu Thu Apr 19 16:01:13 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 19 Apr 2001 16:01:13 \+0100 *F Date: Thu, 19 Apr 2001 11:00:29 \-0400 (EDT) *F From: William Gelbart *F To: gm119@gen.cam.ac.uk *F Subject: Re: help with a chromosome *F Cc: gelbart@morgan.harvard.edu *F Content-Length: 2572 *F Hi Gillian, *F Scientific archeology is such fun!! *F Here's what I can tell you: *F >The chromosome in question is called 'Df(2L)DTD51xD52' in the paper and *F >is in Figure 2 of the paper. *F I haven't seen the paper, but if that's what they wrote, then *F they are wrong. In Maureen Su's senior thesis, she refers *F to 'Df(2L)DTD52-D51'. This is undoubtedly the correct name, and *F derives from a screen that I did a long time ago (in a place *F far away ...) for breakpoints in a restricted region of the *F genome (distal 2L between dpp (22F1-2) and the 24E breakpoint *F of In(2LR)DTD52. *F The gamma-ray mutagenesis involved mutagenizing In(2LR)DTD52, *F dppd-ho homozygous males and crossing them to In(2LR)DTD11, *F dpphr4/ CyO females. *F In the absence of a newly-induced lesion of the proper kind, *F In(2LR)DTD52, dppd-ho/ In(2LR)DTD11, dpphr4 flies have *F wild-type wing posture. I looked for exceptions that had *F heldout wing posture, and recovered a series of these exceptions *F that eventually ended up in 63 different established lines, *F called In(2LR)DTD52-D1 thru In(2LR)DTD52-D63. *F I can't find any notes on the cytology of these, although *F I think I examined several of these ... hoping that the *F rearrangements that disrupt transvection would be of the *F type that broke between 22F and 24E on the In(2LR)DTD52 *F chromosome and that also broke distally on another chromosome *F arm. Largely, my recollection is that this result obtained. *F However, I haven't been able to find notes on the cytogenetics *F yet. (I've moved my office twice in the interim and possibly *F these notes have been misplaced or worse.) *F All of the information up to now is based on my lab notebooks. *F Here's some additional information that I've dredged up from *F my memory ... and so is subject to all the problems of degradation, *F apoptosis and revisionism: *F When Jeff Sekelsky realized that he had an Enhancer of dpp *F that mapped in region 23B-E, I gave him these 63 DTD52 derivatives *F to cross to deletions of this region to see if any might have *F lethal breakpoints in the region that would prove useful in *F mapping and cloning this E(dpp), which we later renamed Mad. *F He found a few (4 or so) DTD52 derivatives that died over a *F big deletion of the region that included Mad. The others, *F as I recall, were translocations or pericentric inversions of *F the type that I had hoped for, but DTD52-D51 turned out from *F complementation analysis and subsequent cytology (by Jeff or by *F me?) to be a small deletion, and hence the renaming as Df(2L)DTD52-D51. *F Hope this is all you need, 'cause it's all I have to tell you. *F Bye, *F Bill # *U FBrf0135983 *a Broadie *b K.S. *t 2001.5.8 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue May 08 16:18:56 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 8 May 2001 16:18:56 \+0100 *F To: broadie@biology.utah.edu *F Subject: FlyBase query: turtle *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 8 May 2001 16:18:56 \+0100 *F Content-Length: 1024 *F Hi Kendal, *F I am curating your paper: *F Bodily et al., J. Neurosci. 21(9):3113--3125 *F which is about the 'turtle' mutation (great name by the way \!!) *F At the beginning of the Results you say that turtle corresponds to *F three CGs, but there has been a typo, so that CG15427 is written twice *F (it says 'CG15426, CG15427, and CG15427'). *F I reckon that you meant: CG15426, CG15427, and CG15428, but I'd be *F grateful if you could confirm this so I can merge the 3 genes in with *F turtle (I know you gave the clot numbers in the methods, but *F unfortunately they change over time, so I don't want to use them for *F any merging), *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From broadie@biology.utah.edu Tue May 08 20:44:00 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 8 May 2001 20:44:00 \+0100 *F Mime-Version: 1.0 *F Date: Tue, 8 May 2001 13:46:03 \-0600 *F To: Gillian Millburn (Genetics) *F From: Kendal Broadie *F Subject: Re: FlyBase query: turtle *F Hi Gillian, *F .. about your query: *F >At the beginning of the Results you say that turtle corresponds to *F >three CGs, but there has been a typo, so that CG15427 is written twice *F >(it says 'CG15426, CG15427, and CG15427'). *F .. Yes, the last one *F should read CG15428. Also, since publication I noticed that CG15426 has *F been renamed l(2)01085 on the Berkely site. *F Kendal *F Dr. Kendal S. Broadie (801) 585-9426 (office; 448 Skaggs) *F Department of Biology (801) 585-9425 (main lab; 450 Skaggs) *F University of Utah (801) 585-1301 (lab II; 460 Skaggs) *F 257 South 1400 East (801) 581-4668 (fax) *F Salt Lake City, Utah 84112-0840 broadie@biology.utah.edu *F lab web page: http://synapse.biology.utah.edu # *U FBrf0136003 *a Pankratz *b M.J. *t 2001.5.11 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Apr 24 17:42:57 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 24 Apr 2001 17:42:57 \+0100 *F To: michael.pankratz@itg.fzk.de *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Tue, 24 Apr 2001 17:42:59 \+0100 *F Content-Length: 1236 *F Dear Dr. Pankratz, *F I am curating the program addendum for the 42nd (Washington, D.C.) *F Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Nutrient control of rotkehichen, a MYST family histone *F acetyltransferase homolog required for food intake and growth in *F Drosophila.' *F You mention a gene that is new to FlyBase, 'rotkehlchen'. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you for your help, *F with best wishes, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \--------------------------------------------------------------- *F From IGEN/mpankratz/michael.pankratz@hikbkem1.fzk.de Fri May 11 11:20:56 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 11 May 2001 11:20:56 \+0100 *F Subject: Re: FlyBase query *F Date: Fre, 11 May 01 12:20:17 \+0100 *F x-sender: IGEN/mpankratz/michael.pankratz@hikbkem1.fzk.de *F x-mailer: Claris Emailer 1.1 *F From: Michael Pankratz *F To: 'Gillian Millburn' *F Mime-Version: 1.0 *F Content-transfer-encoding: quoted-printable *F Content-Type: text/plain; charset='iso-8859-1' *F Content-Length: 378 *F Hello, *F .. *F The gene rotkehlchen (rot) corresponds to CG11290. *F Sincerely, *F Michael Pankratz *F Dr. Michael J. Pankratz *F Institut für Genetik *F Forschungszentrum Karlsruhe *F Postfach 3640 *F 76021 Karlsruhe *F Germany *F Phone (49) 7247-826087 *F Fax (49) 7247-823354 *F e-mail michael.pankratz@itg.fzk.de # *U FBrf0136004 *a Ott *b S.R. *t 2001.3.26 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Mon Mar 26 12:31:40 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 26 Mar 2001 12:31:40 \+0100 *F Date: Mon, 26 Mar 2001 06:31:41 \-0500 (EST) *F From: fbmailer@bio.indiana.edu *F Reply-to: S.R.Ott@cam.ac.uk *F To: flybase-help@morgan.harvard.edu *F Subject: CG14882 \- correction of inferred function *F Content-Length: 726 *F comments: The function currently attributed to CG14882 in FlyBase is that *F of a *F 5-methyltetrahydrofolate--homocysteine S-methyltransferase (methionine *F synthase, EC 2.1.1.13) *F However, blasting CG14882 against GenBank gives *F as closest neighbors (1e-46): *F methinonine synthase REDUCTASE, isoform 1 *F Homo sapiens, accession NP_002445 *F methinonine synthase REDUCTASE, isoform 2 *F Homo sapiens, accession NP_076915 *F This enzyme has methionine synthase as \*substrate* and is reported in *F Ref. PubMed 9501215 *F Best regards, *F S.R. Ott *F mailto: flybase-help@morgan.harvard.edu *F realname: Swidbert R. Ott *F reply-to: S.R.Ott@cam.ac.uk *F Sent from computer 138.37.56.101 # *U FBrf0136005 *a Ashburner *b M. *t 2001.1.10 *T personal communication to FlyBase *u FlyBase error report for CG15767 on Wed Jan 10 08:19:06 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Jan 10 16:19:22 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 10 Jan 2001 16:19:22 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 10 Jan 2001 08:19:06 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ma11@gen.cam.ac.uk *F Subject: FlyBase error report for CG15767 on Wed Jan 10 08:19:06 2001 *F Content-Length: 343 *F Error report from ashburner (ma11@gen.cam.ac.uk) *F Gene or accession: CG15767 *F Missed gene *F Comments: Do a BLAST on NCBI \- the C-term 150 aa are cyclophilin like; *F the N term 200 match fly sns; is this (and sns ?) a fused prediction *F I wonder *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136006 *a Romans *b P. *t 2001.1.29 *T personal communication to FlyBase *u FlyBase error report for CG10655 on Mon Jan 29 16:05:22 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jan 30 00:05:28 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 30 Jan 2001 00:05:28 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 29 Jan 2001 16:05:22 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: victorie@zoo.utoronto.ca *F Subject: FlyBase error report for CG10655 on Mon Jan 29 16:05:22 2001 *F Content-Length: 439 *F Error report from Patricia Romans (victorie@zoo.utoronto.ca) *F Gene or accession: CG10655 *F Missed gene *F Comments: This gene corresponds to the l(2)37Bb gene identified by Wright and *F co-workers. See Stathakis et al. 1995. Genetics 141: 629-655. Note Figure 3. *F It also corresponds to the preliminary sequence Dean Stathakis gave me for the *F l(2)37Bb gene. *F Browser: Mozilla/4.7 en (Win95; I) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136007 *a Mathog *b D. *t 2001.2.2 *T personal communication to FlyBase *u FlyBase error report for AE003714 on Fri Feb 2 09:30:50 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Feb 02 17:30:52 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 2 Feb 2001 17:30:52 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 2 Feb 2001 09:30:50 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: mathog@caltech.edu *F Subject: FlyBase error report for AE003714 on Fri Feb 2 09:30:50 2001 *F Content-Length: 2645 *F Error report from David Mathog (mathog@caltech.edu) *F Gene or accession: AE003714 *F Missed gene *F Comments: There is a small piece of a previously undescribed medium copy and *F apparently *F mobile element at 193592-194128 flanked by Ns from 188413-193591. The entire *F 5716 bp *F insertion may have displaced bases 220929-221003 (75 bp) in *F U31961 when it integrated. Either that or this missing sequence is underneath *F the 'Ns' on the other end of the insertion. U31961 is an earlier sequence of *F the same region, *F supposedly from the same strain. BLASTN shows that this new element is present *F in 15 different Celera derived genomic sequences and appears nowhere else in *F Genbank genomic DNA. It is often found on the ends of contigs, as is expected *F for a mobile element. The sequence is expressed in Schneider cells (BLASTN *F hit against EST database). The piece found in the present entry appears to be *F an LTR like structure, see the table below. BLASTX turned up no similarities *F to the nr *F protein databases. The element is close to 'bxd' and the stock should be checked *F for this phenotype. *F Since this element is previously undescribed I propose it be named *F 'Nuria'. (After my daughter, who is in constant motion.) *F Preliminary analysis. Table of hits of the region indicated above against *F other locations in the Drosophila genome: *F Genbank score E Size Edge? (ref start)start-(ref end)end *F AE003714 1023 0.0 231403 (1)193592-(537)194128 SELF *F AE003615 961 0.0 269823 (1)120718-(537)120186 *F (292)126334-(536)126090 *F AE003777 959 0.0 232000 (1)147584-(536)147053 *F (292)153201-(531)152962 *F AC004377 952 0.0 81677 (1)77304-(536)77835 *F (313)71724-(536)71947 *F AE003836 932 0.0 253273 (1)240409-(537)240942 *F (292)234793-(531)235032 *F AE003804 914 0.0 259817 (1)185733-(537)186265 *F (292)180117-(536)180361 *F AE003601 900 0.0 303626 (53)9158-(537)8678 *F (292)15000-(536)14756 *F AE003482 898 0.0 301769 (1)274684-(537)274152 *F (292)280302-(536)280058 *F AE003457 726 0.0 290792 Y (136)290792-(536)290396 *F (96)290790-(122)290764 *F AE003458 436 e-120 300900 Y (1)195-(198)1 *F (313)5790-(536)5567 *F AE002936 377 e-102 48123 Y (292)47930-(485)48123 *F AE003364 347 4e-93 15042 (1)1612-(536)1112 *F AE003308 283 5e-74 1198 Y (51)8-(535)441 *F AE003494 246 1e-62 299828 (398)138001-(537)137862 *F AE003786 109 1e-21 316758 (292)205472-(374)205390 *F Browser: Mozilla/3.03Gold (X11; I; OpenVMS V7.2-1 COMPAQ AlphaServer DS10 *F 466 MHz) *F Accessed from: FBupdate, /www/hedgehog_8001/htdocs # *U FBrf0136008 *a Duronio *b B. *t 2001.3.7 *T personal communication to FlyBase *u FlyBase error report for CG16988 on Wed Mar 7 10:47:01 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Mar 07 18:47:11 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 7 Mar 2001 18:47:11 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 7 Mar 2001 10:47:01 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: duronio@med.unc.edu *F Subject: FlyBase error report for CG16988 on Wed Mar 7 10:47:01 2001 *F Content-Length: 746 *F Error report from Bob Duronio (duronio@med.unc.edu) *F Gene or accession: CG16988 *F Release: 1 *F Missed gene *F Comments: I deposited this sequence in Genbank as 'ROC2' some time ago. This *F was based on our old (and pre-genome) information that flies had 2 *F 'ROC/RBX/HRT'-like genes. In fact we believe that there are three genes, and *F that there is a different gene than this one with more similarity to a *F vertebrate gene called 'ROC2' in the literature. Therefore, we propose to *F call this gene 'ROC1B', based on its high degree of similarity to 'ROC1', *F which is CG16982. I intend to make these corrections of designation in *F Genbank as well. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136009 *a Whitfield *b E. *t 2001.3.19 *T personal communication to FlyBase *u FlyBase error report for chb on Mon Mar 19 13:54:16 2001. *F From eleanor@ebi.ac.uk Mon Mar 19 13:53:33 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Mar 2001 13:53:33 \+0000 *F Date: Mon, 19 Mar 2001 13:54:16 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 en (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: FlyBase error report for chb! *F Content-Transfer-Encoding: 7bit *F Hi, *F this would be an error report but currently there is no annotation for *F chb! *F In the genes file *F \*a chb *F .. *F there is a translation in AE003593 (which maps to the right region, *F 78C1-2) and chb is encoded by 1 exon ATG (225579-225581) to stop *F (230052-230054). *F Translation is a perfect sequence match to that from AF250842; AAF66060 *F (Lemos et al, EMBO J. 19:3668-3682(2000)) and of course to the BDGP cDNA *F AF195498; AAG28470. *F see you Wed *F Nellie # *U FBrf0136010 *a O'Hare *b K. *t 2001.3.19 *T personal communication to FlyBase *u FlyBase error report for CG2097 on Mon Mar 19 09:08:05 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Mar 19 17:08:12 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 19 Mar 2001 17:08:12 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 19 Mar 2001 09:08:05 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: k.ohare@ic.ac.uk *F Subject: FlyBase error report for CG2097 on Mon Mar 19 09:08:05 2001 *F Content-Length: 1067 *F Error report from Kevin O'Hare (k.ohare@ic.ac.uk) *F Gene or accession: CG2097 *F Release: 1 *F Gene annotation error *F Gene CG2097 has incorrect exon/intron structure or translation start site. *F Comments: Comparison of EST sequences LD45768.3prime and GH21459.3prime with *F genomic *F sequence AE003601.2 indicates an extra intron. Also indicates that this gene *F continues into adjacent entry AE003602.2: *F Entry for CG2097 in AE003601.2 should now read: *F mRNA complement(join(<1..96,203..1125,1202..3324,3386..3579, *F 3650..>3821)) *F /gene='CG2097' *F /product='CT6828' *F gene complement(<1..>3821) *F /gene='CG2097' *F /map='83B9-83C1' *F CDS complement(join(<1..96,203..1125,1202..3324,3386..3579, *F 3650..3736)) *F /gene='CG2097' *F /note='CG2097 gene product' *F Need to change entry for protein_id='AAF51962.1' and include part of CG2097 in *F AE003602.1 *F Browser: Mozilla/4.75 en (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136011 *a Manning *b G. *t 2001.3.20 *T personal communication to FlyBase *u FlyBase error report for CG18214 on Tue Mar 20 09:07:45 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Mar 20 17:07:50 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 20 Mar 2001 17:07:50 \+0000 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 20 Mar 2001 09:07:46 \-0800 (PST) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gerard-manning@sugen.com *F Subject: FlyBase error report for CG18214 on Tue Mar 20 09:07:45 2001 *F Content-Length: 508 *F Error report from Gerard Manning (gerard-manning@sugen.com) *F Gene or accession: CG18214 *F Release: 1 *F Gene annotation error *F Genes CG18214 and trio (FBgn0024277) should be merged. *F Comments: The peptide sequences for trio (gi|6708476) and CG18214 (gi|10727212) *F are virtually identical (2262/2263 match), and only one such sequence is *F found in tblastn search of genome data; CG18214 should be relisted as a synonym *F of trio *F Browser: Mozilla/4.73 en (WinNT; U) *F Accessed from: FBupdate, /www/hedgehog_8001/htdocs # *U FBrf0136012 *a Ross-Macdonald *b P. *t 2001.4.3 *T personal communication to FlyBase *u FlyBase error report for CG10522 on Tue Apr 3 12:29:36 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 03 20:29:43 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 3 Apr 2001 20:29:43 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 3 Apr 2001 12:29:36 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: rossmacp@bms.com *F Subject: FlyBase error report for CG10522 on Tue Apr 3 12:29:36 2001 *F Content-Length: 1254 *F Error report from Petra Ross-Macdonald (rossmacp@bms.com) *F Gene or accession: CG10522 *F Release: 1 *F Gene annotation error *F Gene CG10522 has incorrect exon/intron structure or translation start site. *F Comments: There seem to be two completely non-overlapping transcripts assigned *F to this gene, CT38848 and CT29519. And the scale is weird, the longer one is *F drawn shorter, which is unusual. So it's not clear on the face of it why it *F isn't assigned as two genes. However... *F In the 'interpro motifs' section,protein kinase domains are correctly shown in *F the protein (swiss prot Q9VTY9) from the first, longer transcript CT38848. *F They are also shown in the shorter protein assigned to CT29519 (swissprot *F Q9VTY8). However when the amino acid sequence Q9VTY8 is searched against Pfam, *F this protein has no kinase domains. It has only the CNH domain. *F Maybe these domains were annotated to CT29519 because the transcript was *F translated and searched, rather than the assigned protein Q9VTY8 being used? *F Anyway, the CG10522 kinase domain is homologous to Citron's, and the CNH *F domain is found in citron, so I think both the Kinase and CNH domains should *F be in one protein. *F Browser: Mozilla/4.72 enC-BMY (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136013 *a Jensen *b M.A. *t 2001.4.18 *T personal communication to FlyBase *u FlyBase error report for CG1651 on Wed Apr 18 15:41:33 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 18 23:41:39 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 18 Apr 2001 23:41:39 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 18 Apr 2001 15:41:33 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: majensen@u.washington.edu *F Subject: FlyBase error report for CG1651 on Wed Apr 18 15:41:33 2001 *F Content-Length: 1323 *F Error report from Mark A. Jensen (majensen@u.washington.edu) *F Gene or accession: CG1651 *F Release: 1 *F Gene annotation error *F Gene CG1651 has incorrect exon/intron structure or translation start site. *F Comments: Comparison of the genomic sequence with Ankyrin cDNA (Genbank acc. *F no. L35601) *F as well as the lack of conserved intron start/end signals in the current *F annotation suggests the exon1-intron1-exon2 structure is *F CAATTCTTAATAATTGgtcaacatcttagtaatatttatttctgttaact *F aagataaacttttcttgattcctgtcctttttattgtgcttttaaaatag *F tgataagctataaatacagtaaaaatatgaaacgaaatttattgtaggct *F taaaggaatataaatatgcatttatatatcatatactaattgcgtgaatt *F ttacatacctaaaaaatctttttatttgtatgaaaactttttttttaata *F tttagGCCATTATATTAAATTATGACGCTTGGTGATGTACGAGTTGAGAA *F CAAAATACAAACAAGAAGTGAAACCTGTGCTATCAATGGAATGGCTTTAG *F ACAACAAGAATGGAATCATAAAACAG... *F rather than *F CAATTCTTAATAATTGgtcaacatcttagtaatatttatttctgttaact *F aagataaacttttcTTGATTCCTGTCCTTTTTATTGTGCTTTTAAAATAG *F TGATAAGCTATAAATACAGTAAAAATATGAAACGAAATTTATTGTAGGCT *F TAAAGGAATATAAATATGCATTTATATATCATATACTAATTGCGTGAATT *F TTACATACCTAAAAAATCTTTTTATTTGTATGAAAACTTTTTTTTTAATA *F TTTAGGCCATTATATTAAATTATGACGCTTGGTGATGTACGAGTTGAGAA *F CAAAATACAAACAAGAAGTGAAACCTGTGCTATCAATGGAATGGCTTTAG *F ACAACAAGAATGGAATCATAAAACAG... *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows NT; DigExt) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136014 *a Zhang *b Y. *t 2001.4.24 *T personal communication to FlyBase *u FlyBase error report for CG6203 on Tue Apr 24 22:46:09 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 25 06:46:14 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 06:46:14 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 24 Apr 2001 22:46:09 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: yzhang@biology.utah.edu *F Subject: FlyBase error report for CG6203 on Tue Apr 24 22:46:09 2001 *F Content-Length: 302 *F Error report from yong zhang (yzhang@biology.utah.edu) *F Gene or accession: CG6203 *F Release: 1 *F cDNA or EST error *F Comments: CT17036 has been incorrectly predicted, based on EST sequences of *F AF205596 and AJ271221 *F Browser: Mozilla/4.5 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136015 *a Misra *b S. *t 2001.4.26 *T personal communication to FlyBase *u FlyBase error report for CG7480 on Thu Apr 26 12:18:03 2001. *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 26 20:18:02 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 26 Apr 2001 20:18:02 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 26 Apr 2001 12:18:03 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG7480 on Thu Apr 26 12:18:03 2001 *F Content-Length: 2079 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG7480 *F Release: 1 *F Gene annotation error *F Gene CG7480 has incorrect exon/intron structure or translation start site. *F Comments: Emailed information from Mark Stapleton suggests that CG7480 *F (l(2)35Aa) is annotated on the wrong strand: *F Mark Stapleton wrote: *F I looked into the example you mentioned and found a cluster of 11 AT ESTs *F using an alignment tool called sim4. Within this cluster was AT01079, which *F we have 3' end sequenced. Both the 5' and 3' end reads appear correct in *F there orientation with respect to the cloning vector and the 3' read has a *F polyA tail. Indeed, by BLASTing against the predicted genes, this clone *F appears to be in the opposite orientation. But by using sim4, which aligns *F reads to genomic sequence and therefore not against an assumed oriented gene *F prediction, the reads are 571bp away from each other and on the opposite *F strand from the predicted gene. I can say with some confidence that the *F prediction must be misplaced (wrong strand) especially since the cluster of *F 5' end reads consists of 11 independent clones. *F Hope this helps, *F Mark *F On Thursday 26 April 2001 08:19 am, Henrik Clausen wrote: *F > Dear Dr. Stapleton, *F > *F > We have noticed a cluster of 5' ESTs with apparent reverse orientation. *F > All these *F > ESTs originate from the same testis library, and we can not find *F > information of *F > potential 3' sequencing of the clones. *F > *F > An example is the following: *F > >gb|BG634260.1|BG634260 AT30416.5prime AT Drosophila melanogaster adult *F > *F > testes pOTB7 *F > Drosophila melanogaster cDNA clone AT30416 5 similar to *F > l(2)35Aa: FBan0007480 'enzyme' located on: 2L 35A1-35A1;: *F > *F > If possible we would very much appreciate help with information you may *F > have on *F > this library and/or these clones. Thank you in advance for your help, best *F > regards, *F > Henrik Clausen *F > Henrik Clausen, DDS, DSc *F > Associate professor *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.8 sun4u; Nav) *F Accessed from: FBupdate, /www/hedgehog_8001/htdocs # *U FBrf0136016 *a Williams *b B. *t 2001.5.9 *T personal communication to FlyBase *u FlyBase error report for CG12352 on Tue May 8 23:29:35 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed May 09 07:29:40 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 9 May 2001 07:29:40 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 8 May 2001 23:29:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: bw28@cornell.edu *F Subject: FlyBase error report for CG12352 on Tue May 8 23:29:35 2001 *F Content-Length: 351 *F Error report from Byron Williams (bw28@cornell.edu) *F Gene or accession: CG12352 *F Release: 1 *F Missed gene *F Comments: 'separation anxiety' (san) *F Mutations in san cause defects in mitosis. *F The san protein contains acetyltransferase motifs. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Windows NT; DigExt) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136017 *a Sonoda *b J. *t 2001.4.15 *T personal communication to FlyBase *u FlyBase error report for CG5251 on Sun Apr 15 21:35:28 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 16 05:35:35 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 16 Apr 2001 05:35:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 15 Apr 2001 21:35:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sonod002@mc.duke.edu *F Subject: FlyBase error report for CG5251 on Sun Apr 15 21:35:28 2001 *F Content-Length: 820 *F Error report from Jun Sonoda (sonod002@mc.duke.edu) *F Gene or accession: CG5251 *F Release: 1 *F Gene annotation error *F Genes CG5251 and CG5244 should be merged. *F Protein sequence: *F STEAAPAPAASKPAERVETSESTIRQKKAEVTESCEDNLPQKRLAGTNVQRSVSSCSENSEGHVSESSLSEKSLTGDYV *F EEKCNSVNSESQQESVKFQEELEKSNEAIVEAETILPTAESSEDISPAAVAPGNGEVEGCLPVVDPVEPPSLADNGSRV *F TDALSKLNIFDDTPSFFTSPSFQQAPILKNKLDLEMRQSHLPDLVNDIDGIQKASNTNEWEEAFKNVMMGNTQHMEEQL *F LQQQHLQQHQNLRHQLVLQQEEFLRMQELQKRNNFATQINGPANDFLRAYELRAQANAIIQQQLLQQHAGENLFGGNMS *F KFFDFHKSQPQSHHQYLNGHPPQINGNGAVPEPAKSGGLFGKQSTEFAFVENGLINSQQQQQQQQQQQKQRMMGMYEFM *F PSNTQSQQNRLHRT *F Comments: Above is an incomplete protein sequence assembled from multiple cDNA *F isolated in yeast two hybrid screen. # *U FBrf0136018 *a Mittman *b S. *t 2001.4.17 *T personal communication to FlyBase *u FlyBase error report for AE003436 (CG15899 and CG4222) on Tue Apr 17 08:07:35 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 17 16:07:41 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Apr 2001 16:07:41 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 17 Apr 2001 08:07:35 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: smittman@jhmi.edu *F Subject: FlyBase error report for AE003436 (CG15899 and CG4222) on Tue Apr 17 *F 08:07:35 2001 *F Content-Length: 11673 *F Error report from Scott Mittman (smittman@jhmi.edu) *F Gene or accession: AE003436 (CG15899 and CG4222) *F Release: 2 *F Gene annotation error *F Gene CG15899 and CG4222 has incorrect exon/intron structure or translation *F start site. *F Comments: 1) Comparison with mammalian Ca2+ channel alpha13 subunit sequences *F (NP_06149, NP_066921, NP_066919) suggested that predicted genes CG15899 *F and CG4222 should instead form a single gene. RT-PCR of D. melanogaster mRNA *F (sense primer \- ACTCCTCGCACACCTCGTCA, antisense primer \- GTCGTCCACGCAGGGCTGAT) *F confirmed this suspicion. AE003436 nt 217058-211515 constitute an intron. This *F intron is of the AT-AC type, just like the corresponding introns in the *F mammalian genes. I have not confirmed the putative translation initiation site *F of CG15899/CG4222. *F 2) AE003436 nt 199014-198781 were predicted to form part of an exon. RT-PCR of *F D. melanogaster mRNA (sense primer \- GAACCGGCCACCTGCTATGC, antisense primer \- *F TCCGGCGGCTGATTATGGTA) showed that they are part of an intron. In other words, *F the wrong splice acceptor was predicted. *F 3) AE003436 nt 180921-180860 were predicted to be an intron. However, *F comparison with the mammalian orthologues strongly suggests that these nt are *F part of the exon. These and downstream nt (now frameshifted) encode the 4th, *F 5th and 6th membrane-spanning segments of the 4th homologous repeat domain of *F the calcium channel. As yet, I haven't experimentally verified the new *F prediction, nor have I identified the stop codon. *F >nucleotide *F TCCAGCTCGTCCAGTTCGTCCGGCGAACCGAATCTGCCGTATCCGGGATTCCCCGAGATC *F TCTATCAGGGCGCTGACGCAGTACACCCGGCCGCGCAACTGGTGCCTGATGCTCATCACC *F AATCCATGGTTCGAGCGCGTCTCCATTCTGGTCATCCTGCTCAACTGCATCACTCTCGGC *F ATGTATCAGCCCTGCGTGGACGACGCATGTGTGACGAACCGCTGCAAGATCCTTCAGATC *F TTCGACGACATCATCTTCGCCTTCTTTGCCCTGGAAATGACCATCAAGATGGTGGCCATG *F GGCATCTGTGGCAAGAACACCTATCTGGCAGACTCGTGGAATCGGCTGGACTTCTTCATC *F GTTCTGGCAGGTCTGCTGGAGTACGTGATGCATGTGGAGAATCTCAATCTGACTGCCATA *F CGAACGATTCGAGTGCTGCGTCCGCTGCGGGCCATCAATCGAATACCCAGCATGCGAATC *F CTGGTGATGCTCCTGCTGGACACGCTGCCCATGCTGGGCAATGTCCTGTTGCTCTGCTTT *F TTCGTGTTCTTCATCTTCGGAATTATTGGAGTCCAGCTGTGGGAGGGAATTCTGCGACAG *F CGATGTAGTCTGATGCGAACTGAAGGCATGGTCTATCCACTCACCCTGTCGCAGTACTAC *F GAGTTCTCCAAAGATCAGGACTATATCTGCTCCACGCCAAACGATTCGGGAATGCATCTA *F TGCGGAAATTTCCCGCCCTATCGCATTGGTTCGCTGGTCTGTAATGAGGAGGCCAAGCTG *F TTCGACTTCAATGAGCCGACGAACACGTCCTGCGTCAATTGGAACCAGTACTATACCACC *F TGCAAGCAGAGCGGCGAGAATCCCTTTCAGGGCACGATATCCTTCGACAACATCGGCATG *F GCCTGGGTGGCCATATTCCTGGTCATCTCGCTGGAGGGCTGGACGGACATAATGTATTAC *F GTGCAGGATGCGCACAGCTTTTGGGATTGGATATACTTTGTGCTGCTGATTGTGATTGGT *F TCGTTCTTCATGATCAACCTGTGCCTGGTCGTCATCGCCACGCAATTCTCGGAGACCAAG *F AAACGCGAAATGGAGCGGATGCGACAGGAGCGAGCCCGTTATACATCCACTTCAACCCTG *F GCCAGCAGCACAAATAACTCGGAACCGGCCACCTGCTATGCGGAGATCGTCAAATATATC *F GCCCACCTGTGGCGGCGCTTCAAGAGACGAATGTTAAAGAAGTACAGATTATATAAGTAC *F CAGCGACAGCAGCGCAAGGAGGGCCTACTGCCCAATGCCGACAATTTGACCTTTTCGCCG *F TCGCGGATCAAGTGCCATCATCCCAAGTGCCCCAAGTACAGTAATCGCAAGCCGTCCAGT *F ATTCAGGATCAGATGATTACCGTCATGGTGCCGCTCAATTCGGCTAGCAACAACATTAAT *F AATAACAACAACAACAACAGCAACAGTAGCAACCACAATGGCAGTGGAAACCACACAGCT *F GCCGGCGGTGGCAACAACAATAACAACAACAACAACAACAATGCCGCCAGCTGCACCACA *F GTGGCACTGGTCAATGGGATAAATGGCAGCGCCGCCAGTGTGACCATGTCCTCGGCCCAC *F CATCAGCAACATCAATTGCTGCAACATCAACAGCAGCAACACCAAAACCAACAGCAGCAG *F CAGCAGCAGCAGCAGCACTCCTCGTCGGACAACACGGAGCAATCTCTGGCCCCAGACGGA *F TTGCCCCGGAGTTCCTCGCTGAAAAAATCCACCGCACATCATCAACTGAAGCCGGAGGGC *F AGTGCCGCCGAGCAGAAGACCATATTGCTGAAGTTTCCGCAGCAAATGATTGATTCCGAG *F CAGCTAATTCTGCAGTTGGGAAATTTGGGCAAGAGTCATCCGTGCACCTCGGGCTTCCTA *F AGTCCGCCCACCTCGGCCAGCCGGCGACCTTCGGTGATGTTCAACGAGTACGTGCTGCTC *F CACACGCCGCCTGCCCTCAACGCAGATCCGGCAACGGCGGGCACCACCACAACGGTGGCT *F CCAACAGTCGCAACAGTTGCCGGCACGGCGGCCGCCGCAGCAGCTGCAGCAGCGACAGGA *F TCCGGCAGTGGCAATAACAACCACCAAAATGGCGGTGCAAGTGGAGGAGCTGGAACTGGG *F GGAACCACTGAGAAAAGCAACATTTTCTCAACGGAAAAGATGACCCAAGCTGGCGATGGC *F AGCATCTGGCAGGTTAATCTGCCGCAGACCATCGGCACCATTGCCAATCCCTATGCCGAT *F TGCTCTGAACTTGGTATACACGATGCGATGACTTGTCAAGAGCTCTTAGCATTCTCTGTG *F GCCTTTTCAGCGGCATTGCCGACGGGCCAGAGTACGCTAGAGTCATTCTACACATCGCTG *F GCCCGCTGCGATCCTCACACCGCAGAGGCGTTGCGGGCGCACCACAAGCCGCGCTCAGTG *F CCCACTGGGCAAAATCAAACGACACCGGGCGCGGAAGGAGCCACTGTGATGGTTGCCTCA *F ACGGCTGGTGATACCGGCCAGACACTTCAACCCTCGACAGTGTCGGCAGTGGTGGGCGGA *F ACCATCGATAGCCATGCGGCGAACCATCGTCGAAAGGAGCACCACCAGCAGTCGCACCAC *F CATCATAACAATAACAATACCACCAGTCACAGTCGCAACTATCGCTCACGGCAGGGACAG *F GGAAATTCGCGAATGCGTGAGCCACGCGCCCCCACTGGCAACTATATGGAGGACTATGCG *F TGCTGTTATGATCTCTACCAGAACGCCCTGTCGCCACTGGACGAACGGCCCAGGCAGAGA *F TCGCCGACGACTCGCTGTCTGATCTCCGTGTATCGATGCATGTCGCGCGTGTGCAGCTGG *F ATTCGTCGCTACATCCGGCGATTGGTGGAGCACAAGTACTTCCAGCAGGGCATCCTGTTG *F GCCATCCTGATTAACACGCTGTCCATGGGCATCGAGTACCATAATCAGCCGCCGGAATTG *F ACCGCCATTGTGGAGACGAGCAACGTCGTCTTCTCCGGCATCTTTGCTGTTGAAATGTTA *F CTAAAGGTTGTTGCAGAGGGTCCATTCCGCTACATTGCCAATGGATTCAATGTTTTCGAT *F GGCATCATCGTTATTCTCAGTGCCATTGAGATCTGTCAGACGTTCATGGGCAACGGAACG *F GGTGGTGGTGGCTCCGGACTGTCCGTGTTGCGGACATTCCGGTTGCTGCGAATCCTCAAA *F TTGGTCCGGTTTATGCCCAATCTGCGACGCCAGCTATTCGTGATGCTGCGCACAATGGAC *F AACGTGGCCGTGTTCTTCTCCCTTCTCGTTTTATTCATCTTTATATTCAGTATACTCGGC *F ATGAATCTGTTCGGATGTAAATTCTGCGAGAAAGTTAACGGAGAGATGATTTGCGATCGT *F AAGAATTTCGACAGCCTGCTCTGGGCGCTCGTCACAGTGTTCCAGATACTGACGCAAGAG *F GATTGGAACGTCGTCCTGTTCAACGGAATGGAAAAGACAAGTCATTGGGCCGCATTGTAC *F TTTGTGGCACTAATGACGTTCGGCAATTATGTGCTATTTAATTTATTGGTGGCCATTTTG *F GTTGAGGGATTCAGTTCAGAGCGAAATGAACGTCGCGAGCGCGAACAGCGCGAGTTGGTT *F AAGAAACTGCGTGAGGAGACGTTGGCCGAGAACTACAGCGATGGTATGTACGATGAGTCG *F CGGAGCGAGGCAGACTCCTCGACCACCAACGATAGTTACTACGAGGTGCGCAACCGCTGG *F CGATCGGCGGAGGATGTGCGCAAGCTACAGGACTCCGTCGAGCTGATCATCGAGGCCAAG *F AGCAACATGCACCGCCAGCGCCTGCTGCAGCCCACCCACGATTACCAGATCAACGAGCTG *F CCCGCCTCGTCTGCCGCCCCTTCCGCCTCTGGCACCTCTGGCGCCTCCGCACCTGGCGAC *F CGGGAGAGGGACAGGGACAGAGACAGAGATAGGGAGCGAGACAGAGATAGGGACAGGGAG *F CGGGACAGAGAGGCGGGCGGCGGCGGCAAGGAGGAGGGGGCGCAGCATGCCAAGCCGCGC *F GGCGGCCTCAAAAAGACATACTCCATCAAGGAGCGGCGCAGCGAGGCGCCACGCCTCTCC *F AAGATCCGGCTCGCCCGGGATCCGCCCATCATCACGACAACGGCGGCCACGCCGCAGGAC *F TCGCCCAGCACCACGTTGGAGCCGGGCATGAGCTTCCGCCAGTGGGGCGACATGGAGCCA *F CCCAGTCCGCCCTCTCCGTCGCTTTTGCGACCGCCGAACATCTTTACCGGCGGACAGCGG *F AGTCTGGACGAGGGCATACCCTCCATCGATCTCATACCGCCCTCGCCGGTGCTATCCCAC *F AAGCCCCTGAATATCCTCAATGCCAGCCAGCTGGGCGTGGGCATGGGAGGCGGTGTCCCC *F AGCACCGCCGGCAGTTCGAGCAGCATGCACAGCGTGATCATCGACGACATTTCGAAGAGC *F TCCAGCTCAACGGCGGCGGCTACGCCCATTTATGTGCCCACCATCTCGTCCACAGCGGAC *F GCGCAGAGCCAGAGTCACAGTCTCAACGATGTAAGTGTGGGCTCCAGTCCAGGAGGTGAG *F ATTCCCGCAACGGGAATGAGCAGGAATGCCAACACGGGAGCCTCGACCAGTGGAAGTTCG *F TCCAGCGAGCGCCTGCCATTGGCTCCACCTCCTCAGCAGGGGAGTTTTAAGCAGCGCTTA *F CGGCGCGGTAGCTCCAAAAAGCGACGTGCCTCCGCTTTGGCCCTGGCCACAGACGACAAT *F CCGGCTCGAAGAACGCTGGATAATCAGGCTCGAAGGACCCAGGACGAGGAGGAAGAACAG *F CAGCAGCTGAACAATGGCGGCGATAACAGCTGTTTGCTGAGAAACAGCAACGCTGTGGTC *F AGCGGTTCCTCCTCCGGCACCAAGGAGACCAATCGCCTGAGTCCACAAAACTCCATACGA *F AGGTTATCCAATACACTGAGTATAGGCAGCGGACCGGTGGGCAGTCGTCGGGCATCGGCT *F TGCATTTTCAACTCGCAGGTCTATCAGAATCTAAATCAACCGCCCAAACTGCGTCCAGGG *F TCCGGGCAACGAAGGATGAGCTCCATCGAACTTGCGTTCAGCAAGACTTCGCACCTGAAT *F CTGCACAACTTGGAGGCCAACCGCAAGTCGCTCTCGTATACGAACTCGAAGATGGACCTG *F GACAAGTGGAACAAGTCGTACGGCAATCTGAATGAGCCGGACAACATGCTGCAACAGTAC *F ATGGAGGCGCGGGACAAGCGCAAGAACTCCATCAGCCACTACAACCTAAAGAAGCGGCTC *F GAGGAAAAGGAGCTGCAGCAGCTGCAGCAGCTCCACCAGCAACAGCTGCTCCAGCAACGG *F CAGGACTCCTTCTCCTCGACCACCCAGCAGCAGCAGCAGCAGCTGCAGCAGCACCGCTTG *F TCCAAGGATCAGCAGCAGCTAGCGATGCAGCCACATTCCATGGTGCCAGGAGGCGGCGAG *F CGCTACTCCAAGCTCAAGATGCTCATCGAGCAGCTGACGCCCAAGCACTTTACCACCGAG *F CGCGAGGACTACTCGCTCTACATATTCCCAGAGGACAACAGGTTCCGGCAAATCTGTACG *F TGGTTTGTCAACCAAAAGTGGTTCGACAACGTGGTCCTGCTGTTCATCGCGCTCAATTGC *F ATCACCCTGGCCATGGAAAGACCAAACATCCCTCCAAGCAGCACCGAAAGATTGTTCCTG *F GCAACAGCCAATTACGTGTTCACCGTCGTCTTTACCGTCGAAATGTTTATCAAGGTGGTG *F GCAACGGGAATGTTTTACGGCCACGACGCCTACTTTACGTCCGGCTGGAATATCATGGAC *F GGATCTTTGGTTACCATTTCCATAATTGATTTGTTAATGTCCCTGATTAGCGAATCGAGT *F CCGAGGATATTTGGGATTTTAAGGGTGTTTCGACTACTCCGATCCTTGCGGCCGCTGCGG *F GTGATCAACCGAGCCCCGGGTCTGAAACTAGTCGTGCAAACGCTCTTATCGTCCCTGCGT *F CCCATCGGCAACATCGTGCTGATCTGCTGCACCTTCTTCATCATCTTCGGCATCCTGGGC *F GTTCAGCTCTTCAAGGGCACCTTCTACTACTGCGAGGGAGAGAACATCAAGGGCGTGCGG *F AATGCGGACGAGTGCCGCAGGATTCCTGGCAACGTTTGGACCAACCGCAAGTACAACTTC *F GACGATCTGGGCAAGGCCCTGATGTCTTTATTCGTCCTGAGTTCCCGCGACGGCTGGGTA *F AACATCATGTACACCGGCCTGGATGCGGTGGGCGTCGACCAGCAGCCCATCGTTAACTAT *F AACGAGTGGCGTCTCCTGTACTTCATCGCCTTCATCCTGTTGGTGGGCTTCTTCGTGCTG *F AACATGTTCGTCGGCGTGGTGGTGGAGAACTTCCATCGTTGTCGTGAGGAGCAGGAGAAG *F GAGGAGAAGATCCGGCGGGCGGCGAAGAGGGCTCTGCAGATGGAGAAGAAGCGCCGCCGG *F ATGCACGAGCCACCTTACTACACCAACTACTCACCCACCCGGATGTTCGTTCACAATGTG *F GTGACCTCCAAGTACTTTGATTTAGCCATTGCTGCCGTCATCGGCCTGAACGTGGTCACC *F ATGGCCATGGAGTACTACAAGATGCCATCTGGACTCAAGTATGCACTGAAGATCTTCAAT *F TACTTTTTTACGGCGGTCTTCATACTGGAGGCCAATATGAAGTTGGTCGCTCTGGGCTGG *F AAGCTGTATCTCAAGGACCGCTGGAACCAGCTAGACGTGGGCATTGTCCTGCTCTCGATT *F GTGGGCATTGTACTGGAGGAGCTGGAGACGAACACGCACCAGATTATACCCATCAATCCG *F ACGATTATACGGGTGATGAGGGTACTGCGGATTGCCAGAGTGCTGAAACTCCTGAAGATG *F GCCAATGGCATAAGGGCCCTCCTGGACACCGTGATGCAGGCCCTGCCCCAGGTGGGCAAC *F CTGGGACTACTGTTTTTCCTGCTATTCTTCATATTCGCGGCATTGGGCGTGGAGCTTTTC *F GGGCGACTCGAGTGCTCCGACGAGATTCCCTGTCAGGGATTGGGCGAGCACGCGCACTTC *F GCCAACTTTGGCATGGCTTTCCTCACATTGTTTCGCGTAGCGACTGGCGACAATTGGAAC *F GGCATCATGAAGGATACGCTGAGGGATAATTGCGATGACGCGGCCGATTGCGTGCGCAAT *F TGCTGCGTCAGCTCGGTTATTGCTCCCATATTCTTTGTGATATTCGTGCTAATGGCGCAA *F TTCGTTTTAGTGAACGTCGTCGTGGCTGTACTGATGAAACACCTCGAGGAGAGCCACAAG *F CAAATGGAGGACGAGCTCGATATGGAGGTGGAGCTCGAGCGCGAGCTG *F >protein *F SSSSSSSGEPNLPYPGFPEISIRALTQYTRPRNWCLMLITNPWFERVSILVILLNCITLG *F MYQPCVDDACVTNRCKILQIFDDIIFAFFALEMTIKMVAMGICGKNTYLADSWNRLDFFI *F VLAGLLEYVMHVENLNLTAIRTIRVLRPLRAINRIPSMRILVMLLLDTLPMLGNVLLLCF *F FVFFIFGIIGVQLWEGILRQRCSLMRTEGMVYPLTLSQYYEFSKDQDYICSTPNDSGMHL *F CGNFPPYRIGSLVCNEEAKLFDFNEPTNTSCVNWNQYYTTCKQSGENPFQGTISFDNIGM *F AWVAIFLVISLEGWTDIMYYVQDAHSFWDWIYFVLLIVIGSFFMINLCLVVIATQFSETK *F KREMERMRQERARYTSTSTLASSTNNSEPATCYAEIVKYIAHLWRRFKRRMLKKYRLYKY *F QRQQRKEGLLPNADNLTFSPSRIKCHHPKCPKYSNRKPSSIQDQMITVMVPLNSASNNIN *F NNNNNNSNSSNHNGSGNHTAAGGGNNNNNNNNNNAASCTTVALVNGINGSAASVTMSSAH *F HQQHQLLQHQQQQHQNQQQQQQQQQHSSSDNTEQSLAPDGLPRSSSLKKSTAHHQLKPEG *F SAAEQKTILLKFPQQMIDSEQLILQLGNLGKSHPCTSGFLSPPTSASRRPSVMFNEYVLL *F HTPPALNADPATAGTTTTVAPTVATVAGTAAAAAAAAATGSGSGNNNHQNGGASGGAGTG *F GTTEKSNIFSTEKMTQAGDGSIWQVNLPQTIGTIANPYADCSELGIHDAMTCQELLAFSV *F AFSAALPTGQSTLESFYTSLARCDPHTAEALRAHHKPRSVPTGQNQTTPGAEGATVMVAS *F TAGDTGQTLQPSTVSAVVGGTIDSHAANHRRKEHHQQSHHHHNNNNTTSHSRNYRSRQGQ *F GNSRMREPRAPTGNYMEDYACCYDLYQNALSPLDERPRQRSPTTRCLISVYRCMSRVCSW *F IRRYIRRLVEHKYFQQGILLAILINTLSMGIEYHNQPPELTAIVETSNVVFSGIFAVEML *F LKVVAEGPFRYIANGFNVFDGIIVILSAIEICQTFMGNGTGGGGSGLSVLRTFRLLRILK *F LVRFMPNLRRQLFVMLRTMDNVAVFFSLLVLFIFIFSILGMNLFGCKFCEKVNGEMICDR *F KNFDSLLWALVTVFQILTQEDWNVVLFNGMEKTSHWAALYFVALMTFGNYVLFNLLVAIL *F VEGFSSERNERREREQRELVKKLREETLAENYSDGMYDESRSEADSSTTNDSYYEVRNRW *F RSAEDVRKLQDSVELIIEAKSNMHRQRLLQPTHDYQINELPASSAAPSASGTSGASAPGD *F RERDRDRDRDRERDRDRDRERDREAGGGGKEEGAQHAKPRGGLKKTYSIKERRSEAPRLS *F KIRLARDPPIITTTAATPQDSPSTTLEPGMSFRQWGDMEPPSPPSPSLLRPPNIFTGGQR *F SLDEGIPSIDLIPPSPVLSHKPLNILNASQLGVGMGGGVPSTAGSSSSMHSVIIDDISKS *F SSSTAAATPIYVPTISSTADAQSQSHSLNDVSVGSSPGGEIPATGMSRNANTGASTSGSS *F SSERLPLAPPPQQGSFKQRLRRGSSKKRRASALALATDDNPARRTLDNQARRTQDEEEEQ *F QQLNNGGDNSCLLRNSNAVVSGSSSGTKETNRLSPQNSIRRLSNTLSIGSGPVGSRRASA *F CIFNSQVYQNLNQPPKLRPGSGQRRMSSIELAFSKTSHLNLHNLEANRKSLSYTNSKMDL *F DKWNKSYGNLNEPDNMLQQYMEARDKRKNSISHYNLKKRLEEKELQQLQQLHQQQLLQQR *F QDSFSSTTQQQQQQLQQHRLSKDQQQLAMQPHSMVPGGGERYSKLKMLIEQLTPKHFTTE *F REDYSLYIFPEDNRFRQICTWFVNQKWFDNVVLLFIALNCITLAMERPNIPPSSTERLFL *F ATANYVFTVVFTVEMFIKVVATGMFYGHDAYFTSGWNIMDGSLVTISIIDLLMSLISESS *F PRIFGILRVFRLLRSLRPLRVINRAPGLKLVVQTLLSSLRPIGNIVLICCTFFIIFGILG *F VQLFKGTFYYCEGENIKGVRNADECRRIPGNVWTNRKYNFDDLGKALMSLFVLSSRDGWV *F NIMYTGLDAVGVDQQPIVNYNEWRLLYFIAFILLVGFFVLNMFVGVVVENFHRCREEQEK *F EEKIRRAAKRALQMEKKRRRMHEPPYYTNYSPTRMFVHNVVTSKYFDLAIAAVIGLNVVT *F MAMEYYKMPSGLKYALKIFNYFFTAVFILEANMKLVALGWKLYLKDRWNQLDVGIVLLSI *F VGIVLEELETNTHQIIPINPTIIRVMRVLRIARVLKLLKMANGIRALLDTVMQALPQVGN *F LGLLFFLLFFIFAALGVELFGRLECSDEIPCQGLGEHAHFANFGMAFLTLFRVATGDNWN *F GIMKDTLRDNCDDAADCVRNCCVSSVIAPIFFVIFVLMAQFVLVNVVVAVLMKHLEESHK *F QMEDELDMEVELEREL *F Browser: Mozilla/4.7 en (WinNT; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0136019 *a Davis *b T. *t 2001.5.1 *T personal communication to FlyBase *u FlyBase error report for CG14307 on Tue May 1 03:43:32 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue May 01 11:44:04 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 1 May 2001 11:44:04 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 1 May 2001 03:43:32 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@caediff.ac.uk *F Subject: FlyBase error report for CG14307 on Tue May 1 03:43:32 2001 *F Content-Length: 1960 *F Error report from Terence Davis (davist2@caediff.ac.uk) *F Gene or accession: CG14307 *F Release: 1 *F Gene annotation error *F Gene CG14307 has incorrect exon/intron structure or translation start site. *F Comments: I have an annotation correction for gene CG14307. This gene is part *F of the fruitless locus (FlyBase ID FBgn0004652) and is a single exon. This is *F an alternative splice form of the fru gene called fru type E (see Usui-Aoki K, *F Ito H, Ui-Tei K, Takahashi K, Lukacsovich T, Awano W, Nakata H, Piao ZF, *F Nilsson EE, Tomida J, Yamamoto D. Formation of the male-specific muscle in *F female Drosophila by ectopic fruitless expression. Nat Cell Biol. 2000 *F Aug;2(8):500-6.). *F With reference to the genomic sequence AE003722 this exon is: *F 37789..37245 *F stop codon at 37243..37245 *F and splices from 45381 *F (these are complementary strand of AE003722). *F The peptide sequence is below: this starts at nucleotide 37787 (37789-37788 *F are second and third bases of previous codon) *F SKAWHMRLTFERLSGGCNLHRCKLCGKVVTHIRNHYHVHFPGRFECPLCRATYTRSDNLRTHCKFKHPMYNPDTRKFDN *F LMSSSAVGAAATPTASQLAVASQAAMAAAAAAAFNAAQQQQQQQQQQQQHQHQQQQQHQQSQQQQQQQQQSQQQLHALA *F QQHMLQLQPQHHQQQQHNATSE *F Please note that the above peptide is longer than that from the given *F reference and is a comparison from the type e protein in D. heteroneura. I *F believe as the genomic sequence between melanogaster (AE003722) and *F heteroneura (AF051664) are so similar that a mistake has been made with the *F cDNA in the reference. *F There is also a type d protein: *F This is an extension of one of the exons in CG7689 *F Thus 45513..45373 (instead of 45513..45381) *F Stop codon 453375..45373 *F This truncates the fru protein and it ends with aa VE (see above ref) *F To summarise the various fru alternative 3’ forms: *F Type A: gene CG7689 *F Type B: gene CG7688 *F Type C: (see FBrf0129326) *F Type D: (above) *F Type E: gene CG14307 # *U FBrf0136020 *a Goodliffe *b J. *t 2001.4.7 *T personal communication to FlyBase *u FlyBase error report for CG6457 on Sat Apr 7 12:15:41 2001. *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 07 20:15:46 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 7 Apr 2001 20:15:46 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 7 Apr 2001 12:15:41 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: julieg@jhu.edu *F Subject: FlyBase error report for CG6457 on Sat Apr 7 12:15:41 2001 *F Content-Length: 729 *F Error report from Julie Goodliffe (julieg@jhu.edu) *F Gene or accession: CG6457 *F Release: 1 *F Assembly error *F Comments: The cDNA for this gene hybridizes to P1 clones DS03834, DS07998 and *F DS07281, all of which are mapped to 77E1-E3. In addition, the cDNA recognizes *F a RFLP in genomic DNA of l(3)04521 insertion mutants, which is mapped to the *F same region. l(3)04521 is inserted 5' of CG4825, as determined by blasting *F l(3)04521 plasmid rescue sequence to both Celera and BDGP genomic sequencing *F data. I propose that CG6457 is near to CG4825 in the region of 77E1-3, based *F on what I have told you and even more evidence. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136021 *a Nelson *b D. *t 2001.2.5 *T personal communication to FlyBase *u *F [FlyBase curator comment: sequence of Drosophila melanogaster P450 *F protein sequences, taken from web page *F http://drnelson.utmem.edu/droso.all.P450s.html on 5.2.2001.] *F >4C3 AC014779 73845-80196 also AC008219 *F MSSKVITSLMAESILLSKVGQVISGYSPITVFLLGSILIFLVVYNKRRSRLVKYIEKIPG *F PAAMPFLGNAIEMNVDHDELFNRVIGMQKLWGTRIGINRVWQGTAPRVLLFEPETVEPIL *F NSQKFVNKSHDYDYLHPWLGEGLLTSTDRKWHSRRKILTPAFHFKILDDFIDVFNEQSAV *F LARKLAVEVGSEAFNLFPYVTLCTLDIVCETAMGRRIYAQSNSESEYVKAVYGIGSIVQS *F RQAKIWLQSDFIFSLTAEYKLHQSYINTLHGFSNMVIRERKAELAILQENNNNNNNNAPD *F AYDDVGKKKRLAFLDLLIDASKEGTVLSNEDIREEVDTFMFEGHDTTSAAISWTLFLLGC *F HPEYQERVVEELDSIFGDDKETPATMKNLMDMRYLECCIKDSLRLFPSVPMMARMVGEDV *F NIGGKIVPAGTQAIIMTYALHRNPRVFPKPEQFNPDNFLPENCAGRHPFAYIPFSAGPRN *F CIGQKFAILEEKAVISTVLRKYKIEAVDRREDLTLLGELILRPKDGLRVKITPRD *F >4d1 AC017770 49988-51720 *F MFLVIGAILASALFVGLLLYHLKFKRLIDLISYMPGPPVLPLVGHGHHFIGKPPHEMVKK *F IFEFMETYSKDQVLKVWLGPELNVLMGNPKDVEVVLGTLRFNDKAGEYKALEPWLKEGLL *F VSRGRKWHKRRKIITPAFHFKILDQFVEVFEKGSRDLLRNMEQDRLKHGDSGFSLYDWIN *F LCTMDTICETAMGVSINAQSNADSEYVQAVKTISMVLHKRMFNILYRFDLTYMLTPLARA *F EKKALNVLHQFTEKIIVQRREELIREGSSQESSNDDADVGAKRKMAFLDILLQSTVDERP *F LSNLDIREEVDTFMFEGHDTTSSALMFFFYNIATHPEAQKKCFEEIRSVVGNDKSTPVSY *F ELLNQLHYVDLCVKETLRMYPSVPLLGRKVLEDCEINGKLIPAGTNIGISPLYLGRREEL *F FSEPNIFKPERFDVVTTAEKLNPYAYIPFSAGPRNCIGQKFAMLEIKAIVANVLRHYEVD *F FVGDSSEPPVLIAELILRTKEPLMFKVRERVY *F >4d1 alt. splice AC017770 48817-51720 *F MWLLLSLVLLLAIIALEMRRFLRNMRTIPGPLPLPLLGNAHIFLGLTPAEACLKIGELAE *F RHGDTFGLFLGPSYSVMLFNPRDVERVLGSSQLLTKSQEYSFLGRWLNEGLLVSNGRKWH *F RRRKIITPAFHFRILEPYVEIFDRQSLRLVEELALRISRGQERINLGEAIHLCALDAICE *F TAMGVSINAQSNADSEYVQAVKTISMVLHKRMFNILYRFDLTYMLTPLARAEKKALNVLH *F QFTEKIIVQRREELIREGSSQESSNDDADVGAKRKMAFLDILLQSTVDERPLSNLDIREE *F VDTFMFEGHDTTSSALMFFFYNIATHPEAQKKCFEEIRSVVGNDKSTPVSYELLNQLHYV *F DLCVKETLRMYPSVPLLGRKVLEDCEINGKLIPAGTNIGISPLYLGRREELFSEPNIFKP *F ERFDVVTTAEKLNPYAYIPFSAGPRNCIGQKFAMLEIKAIVANVLRHYEVDFVGDSSEPP *F VLIAELILRTKEPLMFKVRERVY *F >4d2 AC017770 comp(32267-34028) *F MLGVVGVLLLVAFATLLLWDFLWRRRGNGILPGPRPLPFLGNLLMYRGLDPEQIMDFVKK *F NQRKYGRLYRVWILHQLAVFSTDPRDIEFVLSSQQHITKNNLYKLLNCWLGDGLLMSTGR *F KWHGRRKIITPTFHFKILEQFVEIFDQQSAVMVEQLQSRADGMTPINIFPVICLTALDII *F AETAMGTKINAQKNPNLPYVQAVNDVTNILIKRFIHAWQRVDWIFRLTQPTEAKRQDKAI *F KVMHDFTENIIRERRETLVNNSKETTPEEEVNFLGQKRRMALLDVLLQSTIDGAPLSDED *F IREEVDTFMFEGHDTTTSAISFCLYEISRHPEVQQRLQQEIRDVLGEDRKSPVTLRDLGE *F LKFMENVIKESLRLHPPVPMIGRWFAEDVEIRGKHIPAGTNFTMGIFVLLRDPEYFESPD *F EFRPERFDADVPQIHPYAYIPFSAGPRNCIGQKFAMLEMKSTVSKLLRHFELLPLGPEPR *F HSMNIVLRSANGVHLGLKPRA *F >4d8 AC017388 comp(37292-39859) also AC010557 AC010112 AC010113 *F MLLFLLVVLLFGAGWIIHLGQADRRRKVANLPGPICPPLIGAMQLMLRLNPKTFIKVGRE *F YVLKFGHLQRVWIFNRLLIMSGDAELNEQLLSSQEHLVKHPVYKVLGQWLGNGLLLSDGK *F VWHQRRKIITPTFHFSILEQFVEVFDQQSNICVQRLAQKANGNTFDVYRSICAAALDIIA *F ETAMGTKIYAQANESTPYAEAVNECTALLSWRFMSVYLQVELLFTLTHPHLKWRQTQLIR *F TMQEFTIKVIEKRRQALEDQQSKLMDTADEDVGSKRRMALLDVLLMSTVDGRPLTNDEIR *F EEVDTFMFEGHDTTTSALSFCLHELSRHPEVQAKMLEEIVQVLGTDRSRPVSIRDLGELK *F YMECVIKESLRMYPPVPIVGRKLQTDFKYTHSVHGDGVIPAGSEIIIGIFGVHRQPETFP *F NPDEFIPERHENGSRVAPFKMIPFSAGPRNCIGQKFAQLEMKMMLAKIVREYELLPMGQR *F VECIVNIVLRSETGFQLGMRKRKHN *F >4d14 AC017770 35699-37438 *F MYLELFAILLATALAWDYMRKRRHNKMYAEAGIRGPKSYPLVGNAPLLINESPKTIFDMQ *F FRLIAEFGKNIKTQMLGESGFMTADSKMIEAIMSSQQTIQKNNLYSLLVNWLGDGLLISQ *F GKKWFRRRKIITPAFHFKILEDFVEVFDQQSATMVQKLYDRADGKTVINMFPVACLCAMD *F IIAETAMGVKINAQLQPQFTYVQSVTTASAMLAERFMNPLQRLDFTMKLFYPKLLDKLND *F AVKNMHDFTNSVITERRELLQKAIADGGDADAALLNDVGQKRRMALLDVLLKSTIDGAPL *F SNDDIREEVDTFMFEGHDTTTSSIAFTCYLLARHPEVQARVFQEVRDVIGDDKSAPVTMK *F LLGELKYLECVIKESLRLFPSVPIIGRYISQDTVLDGKLIPADSNVIILIYHAQRDPDYF *F PDPEKFIPDRFSMERKGEISPFAYTPFSAGPRNCIGQKFAMLEMKSTISKMVRHFELLPL *F GEEVQPVLNVILRSTTGINCGLKPRVY *F >4d20 AC019509 comp(19731-21636) *F MWLTLITGALILLLTWDFGRKRQRVLAFEKSAIPGPISIPILGCGLQALHLGAENIIGWV *F GEKFDKYGKTFRFWILGESLIYTKDLQYFETILSSTTLLEKGQLYEYLRPFLNDGLLVST *F GRKWHARRKIFTHAFHFKVLEHYVEIMDRHSSVMVDNLRKVADGKTAVDMLKYVSLAALD *F VITEAAMGVQVNAQNDPDFPYIKALKSVVYIQPDRMFRFSRRYNWLFPLAAPLLHRQLLS *F DIRVMHDFTDKVISERRETVRRAKADGTYRPLSLGDAEIGSKSQMALLDILLQSSINNQP *F LSDADIREEVDTFMFEGDDTTSSGVSHALYAIARHPEVQQRIFEELQRVLGPDASAPVTQ *F AQLQDLKYLDCVIKETMRLYPPVPAIGRHAQKELEIGDKTIPANTSIYLVLYYAHRDANY *F FPDPLSFRPERFLEDQEQGHNTFAYVPFSAGPKNCIGQKFAVLEMKVLISKVLRFYELLP *F LGEELKPMLNFILRSASGINVGLRPRKALR *F >4d21 AC020004 55466-57280 *F MWILLGIAVLIMTLVWDNSRKQWRVNTFEKSRILGPFTIPIVGNGLQALTLRPENFIQRF *F GDYFNKYGKTFRLWILGECLIYTKDLKYFESILSSSTLLKKAHLYRFLRDFLGDGLLLST *F GNKWTSRRKVLAPAFHFKCLENFVEIMDRNSGIMVEKLKNYADGKTCVDLFKFVSLEALD *F VTTETAMGVQVNAQNEPNFPYTKALKSVVYIESKRLASVSMRYNWLFPLAAPLVYRRLQK *F DIAIMQDFTDKVIRERRAILERARADGTYKPLIMGDDDIGGKAKMTLLDILLQATIDNKP *F LSDVDIREEVDVFIFAGDDTTTSGVSHALHAISRHPKVQECIYEELVSVLGPDPDASVTQ *F TKLLELKYLDCVIKETMRLHPPVPILGRYIPEDLKIGEITIPGNTSILLMPYYVYRDPEY *F FPDPLVFKPERWMDMKTTSNTPPLAYIPFSSGPKNCIGQKFANLQMKALISKVIRHYELL *F PLGADLKATYTFILSSSTGNNVGLKPRTRVK *F >4e1 AC020402 44580-46698 *F MWIVLCAFLALPLFLVTYFELGLLRRKRMLNKFQGPSMLPLVGNAHQMGNTPTEILNRFF *F GWWHEYGKDNFRYWIGYYSNIMVTNPKYMEFILSSQTLISKSDVYDLTHPWLGLGLLTST *F GSKWHKHRKMITPAFHFNILQDFHEVMNENSTKFIDQLKKVADGGNIFDFQEEAHYLTLD *F VICDTAMGVSINAMENRSSSVVQAFKDITYTIKMRAFSPWKRNKYLFHFAPEYPEYSKTL *F KTLQDFTNEIIAKRIEVRKSGLEVGIKADEFSRKKMAFLDTLLSSKVDGRPLTSQELYEE *F VSTFMFEGHDTTTSGVGFAVYLLSRHPDEQEKLFNEQCDVMGASGLGRDATFQEISTMKH *F LDLFIKEAQRLYPSVPFIGRFTEKDYVIDGDIVPKGTTLNLGLLMLGYNDRVFKDPHKFQ *F PERFDREKPGPFEYVPFSAGPRNCIGQKFALLEIKTVVSKIIRNFEVLPALDELVSKDGY *F ISTTLGLQPAEKKSRDAHNHKYDPILSASMTLKSENGLHLRMKQRLVCDST *F >4e2 AC020402 41848-44099 *F MWFVLYIFLALPLLLVAYLELSTFRRRRVLNKFNGPRGLPLMGNAHQMGKNPSEILDTVF *F SWWHQYGKDNFVFWIGTYSNVLVTSSKYLEFILSSQTLITKSDIYQLTHPWLGLGLLTST *F GSKWHKHRKMITPAFHFNILQDFHEVMNENSTKFIKHLKTVAAGDNIFDFQEQAHYLTLD *F VICDTAMGVSINAMENRSSSIVQAFKDMCYNINMRAFHPLKRNELLYRLAPDYPAYSRTL *F KTLQDFTNEIIAKRIEAHKSGAVSTNAGDEFTRKKMAFLDTLLSSTIDGRPLNSKELYEE *F VSTFMFEGHDTTTSGVSFAVYLLSRHQDEQRKLFKEQREVMGNSELGRDATFQEISQMKY *F LDLFIKEAQRVYPSVPFIGRFTEKDYVIDGDLVPKGTTLNLGLVMLGYNEKVFKDPHKFR *F PERFELEKPGPFEYVPFSAGPRNCIGQKFALLEIKTVVSKIIRNFEVLPALDELVSKDGY *F ISTTIGLPDAERKKRDPYRHKYDPILSAVLTLKSENGLYIRLKERH *F >4e3 AC020324 73117-75125 *F MWLAVLALLVLPLITLVYFERKASQRRQLLKEFNGPTPVPILGNANRIGKNPAEILSTFF *F DWWYDYGKDNFLFWIGYSSHIVMTNPKQLEYILNSQQLIQKSTIYDLLHPWLGHGLLTSF *F GSKWHKHRKMITPSFHFNILQDFHEVMNENSAKFMTQLKKASAGDTIIDFQEHANYLTLD *F VICDTAMGVPINAMEQRDSSIVQAFRDMCYNINMRAFHPFKRSNRVFSLTPEFSAYQKTL *F KTLQDFTYDIIEKRVYALQNGGSKEDHDPSLPRKKMAFLDTLLSSTIDGRPLTRQEIYEE *F VSTFMFEGHDTTTSGVSFSVYLLSRHPDVQRKLYREQCEVMGHDMNRSVSFQEIAKMKYL *F DLFIKEAQRVYPSVPFIGRYCDKDYDINGSIVPKGTTLNLALILLGYNDRIFKDPHHFRP *F ERFEEEKPAPFEYLPFSAGPRNCIGQKFALLELKTVISKVVRSFEVLPAVDELVSTDGRL *F NTYLGLAPDEKLKREAGRHKYDPILSAVLTLKSDNGLHLRLRERRS* *F >4g1 AC020093 comp(33262-34932) no introns *F MAVEVVQETLQQAASSSSTTVLGFSPMLTTLVGTLVAMALYEYWRRNSREYRMVANIPSP *F PELPILGQAHVAAGLSNAEILAVGLGYLNKYGETMKAWLGNVLLVFLTNPSDIELILSGH *F QHLTKAEEYRYFKPWFGDGLLISNGHHWRHHRKMIAPTFHQSILKSFVPTFVDHSKAVVA *F RMGLEAGKSFDVHDYMSQTTVDILLSTAMGVKKLPEGNKSFEYAQAVVDMCDIIHKRQVK *F LLYRLDSIYKFTKLREKGDRMMNIILGMTSKVVKDRKENFQEESRAIVEEISTPVASTPA *F SKKEGLRDDLDDIDENDVGAKRRLALLDAMVEMAKNPDIEWNEKDIMDEVNTIMFEGHDT *F TSAGSSFALCMMGIHKDIQAKVFAEQKAIFGDNMLRDCTFADTMEMKYLERVILETLRLY *F PPVPLIARRLDYDLKLASGPYTVPKGTTVIVLQYCVHRRPDIYPNPTKFDPDNFLPERMA *F NRHYYSFIPFSAGPRSCVGRKYAMLKLKVLLSTIVRNYIVHSTDTEADFKLQADIILKLE *F NGFNVSLEKRQYATVA *F >4g15 AC013897 comp(8972-10574 and 5306-6643) two pieces *F MEVLKKDAALGSPSSVFYFLLLPTLVLWYIYWRLSRAHLYRLAGRLPGPRGLPIVGHLFD *F VIGPASSVFRTVIRKSAPFEHIAKMWIGPKLVVFIYDPRDVELLLSSHVYIDKASEYKFF *F KPWLGDGLLISTGQKWRSHRKLIAPTFHLNVLKSFIELFNENSRNVVRKLRAEDGRTFDC *F HDYMSEATVEILLETAMGVSKKTQDKSGFEYAMAVMRMCDILHARHRSIFLRNEFVFTLT *F RYYKEQGRLLNIIHGLTTKVIRSKKAAFEQGTRGSLAQCELKAAALEREREQNGGVDQTP *F STAGSDEKDREKDKEKASPVAGLSYGQSAGLKDDLDVEDNDIGEKKRLAFLDLMLESAQN *F GALITDTEIKEQVDTIMFEGHDTTAAGSSFFLSLMGIHQDIQDRVLAELDSIFGDSQRPA *F TFQDTLEMKYLERCLMETLRMYPPVPLIARELQEDLKLNSGNYVIPRGATVTVATVLLHR *F NPKVYANPNVFDPDNFLPERQANRHYYAFVPFSAGPRSCVGRKYAMLKLKILLSTILRNY *F RVYSDLTESDFKLQADIILKREEGFRVRLQPRTS *F >4p1 AC018129 comp(67703-69662) *F MIILWLILALSALLYWLHRANKDYHILSFFTKRIRLKDGTPVEIIAPIAKGKTIFGNTLD *F LYGRDHAGVFNYSRERAKEMGTSYIEYVFGKAIYNIIDADSAENVLNHPNLITKGLVYNF *F LHPFLRTGLLTSTGKKWHARRKMLTPTFHFNILNQFQEIFKTESQKFLLQFEGQDEVTIT *F LHDVIPRFTLNSICETAMGVKLDEMAEKGDRYRENFSQIEECFIRRLSNPLLWGDKLFEM *F FAAKDFASALDVVHRFSSEIIAKRRDLLKDELDKSSSTADDDGFVSKKRFAMLDTLIYAE *F KDGLIDHIGICEEVDTLMFEGYDTTSIGLIFGLMNMSLNPDKQELCYQEIQEHIDDDLSN *F LDVGQLNKLKYLEYFMKETTRLFPSVPIMGREAVQETELANGLILPKGAQITIHVFDIHR *F NAKYWDSPEEFRPERFLPENVQDRHTYAYVPFSAGQRNCIGKKYAMQEMKTLMVVLLKQF *F KVLKAIDPQKIVFHTGITLRTQDKIRVKLVRRT *F >4p2 AC018129 comp(70087-72192) revised 9/11/2000 *F MMICLLWISVAILVVIHWIYKVNKDYNILAFFARRVQTKDGKPLDSLVPMIKGRTVFANC *F FDLLGKDTDQVFTHLRQLAKNSGDSYLQYSMGFSNFNVIDAHNAANILNHPNLITKGVIY *F NFLHPFLRTGVLTATEKKWHTRRSMLTRTFHLDILNQFQEIFIAESLKFVSQFQGQNEVV *F VSLKDRISRFTLNSICETAMGIKLDEMAEKGDRYRANFHIIDEGLTRRIVNPLYWDDCVY *F NMFTGHKYNAALKVVHEFSREIIAKRRVLLEEELENRRATQTADDDICVIRKKRFAMLDT *F LICAEKDGLIDDIGISEEVDTLMAEGYDTTSIGLVFGLMNMSLYAAEQELCYQEIQEHIL *F DDLSNLNLSQLSKLNYLGYFIKETMRLYPSIPIMGRQTLQETELENGLILPKRSQINIHV *F FDIHRNPKYWESPEEFRPERFLPQNCLKRHPYAYIPFSAGQRNCIGQKYAMQEMKTLMVV *F ILKHFKILPVIDPKSIVFQVGITLRFKNKIKVKLVRRNCV *F >4p3 AC018129 comp(65426-67385) revised 9/11/2000 *F MLILWLVGAFIVLIQWIYRLNRDYCILGFFAKRIRTKNGQNPESIAPLVKGSTIFANSFD *F LYGKDHSGVFEHSRDCAKKLGKSYAEYAMGTAIYNVIDADSAERVLNDPNLINKGTIYDF *F LHPFLRTGLLTSTGKKWHARRKMLSPTFHFNILNQFQEIFITESLKFLEQFKGNDEAIIS *F LNEVIPRFTLNSICETAMGVKLDEMAEKGDRYRENFRQIEECFIRRMSNPLLWSDTLFKM *F FAEKDYASALDVVHGFSSEIIAKRRDQLNDEIDSRGNTQTAEDELFTSKKRFAMLDTLIL *F AEKDGLIDHIGICEEVDTLMFEGYDTTSIGLMFGLMNMSLYPEEQEKCYQEIQANIDDEL *F NILNIGQLNKLKNLEYFIKETMRLFPSVPAMGRETTRETELSNGLILPKGSQIFVHVFDI *F HRNPEYWDSPEEFRPERFLPENSQNRHTYAYIPFSAGQRNCIGQKFAMQEMKTLMVALLK *F QFQILPEIDPKTIVFQTGLTLRTKNQIHVNS *F >4s3 AC018488 comp(98620-101060) also AC015418 AC018487 *F MSTLALVAFVLWAAFLRYLPKILNFLRLQRFAKTLPGPTIGELIANVKKGEILNWLKELR *F EKHGPVFRIWFGKDLMVMFTDPEDIKQLLGNNQLLTKSRNYELLEPWLGKGLLTNGGESW *F HRRRKLLTPGFHFRILSEFKEPMEENCRILVRRLRTKANGESFDIYPYITLFALDAICET *F AMGIKKHAQLQSDSEYVQAVQSICRVMHKQSFSFWQRLNVFFKHTKPGKEREAALKVLHD *F ETNRVIRLRREQLIQERNEWKPEAEQDDVGAKRRLAFLDMLLLTQMEGGAELSDTDIREE *F VDTFMFEGHDTTSSAIAFALSLLSKNPDVQQRAFEEASELEGREKESMPYLEAVIKETLR *F IYPSVPFFSRKVLEDLEVGKLTVPKGASISCLIYMLHRDPKNFPDPERFDPDRFLVNEKQ *F MHPFAFAAFSAGPRNCIGQKFAMLELKTSLAMLLRSYRFLPDKDHQPKPLAELVTKSGNG *F IRLRILPRDENGTTA *F >4aa1 AC020355 comp(15477-16779) and AC007579 comp(38212-38842) *F MHLRLLSPPQLERTTNLELCSILILLVISLSIYTFYATLNTYLRSVLLSLRLTGPPSLPF *F LGNCMLVTDKDLMRRCAGKAFDLYGSLVRIWVLLFPFFAVLEPEDLQVILSSKKHTNKVF *F FYRLMHNFLGDGLITSSGSKWSNHRRLIQPAFHHNLLEKFIDTFVDASQSLYENLDAEAV *F GTEINIAKYVNNCVLDILNEAVLGVPIKKRGQDVAMMEDSPFRQGKIMMPARFTQPWLLL *F DGIYHWTKMANDELNQKKRLNDFTRKMIQRRRQIQNNNNGNSERKCLLDHMIEISESNRD *F FTEEDIVNEACTFMLAGQDSVGAAVAFTLFLLTQNPECQDRCVLELATIFEDSNRAPTMT *F DLHEMRYMEMCIKEALRLYPSVPLIARKLGEEVRLAKHTLPAGSNVFICPYATHRLAHIY *F PDPEKFQPERFSPENSENRHPYAFLPFSAGPRYCIGNRFAIMEIKTIVSRLLRSYQLLPV *F TGKTTIAATFRITLRASGGLWVRLKERDHPLIAH *F >4ac1 AC017771 comp(24745-26507) *F MWIALLGIPILLAVLTLLLKHINKTYFILSLTKRVRTEDGSPLESKVAIMPGKTRFGNNL *F DILNFTPASVFNFVRESTAKAKGQNYLWYFLYAPMYNVVRPEEAEEVFQSTKLITKNVVY *F ELIRPFLGDGLLISTDHKWHSRRKALTPAFHFNVLQSFLGIFKEECKKFLNVLEKNLDAE *F LELNQVIPPFTLNNICETALGVKLDDMSEGNEYRKAIHAIEEVLIQRVCNPLMYYNWYFF *F VYGDYRKHLQNLRIVHDFSSRIIERKRQQFQQKQLGEVDEFGRKQRYAMLDTLLAAEADG *F QIDHQGICDEVNTFMFEGYDTTSTCLIFTLLMLALHEDVQKKCYEEVENLPEDSDDISMF *F QFNKLVYLECVIKESLRMFPSVPFIGRQCVEETVVNGMVMPKDTQISIHIYDIMRDPRHF *F PKPDLFQPDRFLPENTVNRHPFAYVPFSAGQRNCIGQKFAILEMKVLLAAVIRNFKLLPA *F TQLEDLTFENGIVLRTQENIKVKLSKRVK* *F >4ac2 AC017771 comp(22220-24109) revised 9/8/2000 *F MFLEVLFAAPLVIFIFRKLWAHLNRTYFILSLCKRIRTEDGSLLESKIYVAPSKTRFGNN *F FDLVNFTSESIFNFMRDASAKAKGRNYLWYFFHAPMYNIVRAEEAEEILQSSKLITKNMI *F YELLKPFLGEGLLISTDQKWHSRRKALTPAFHFKVLQSFLIIFKEECNKLVKVLHQSVNM *F ELELNQVIPQFTLNNVCETALGVKLDDLSEGIRYRQSIHAIEEVMQQRLCNPFFYNIVYF *F FLFGDYRKQVNNLKIAHEFSSNIIEKRRSLFKSNQLGQEDEFGKKQRYAMLDTLLAAEAD *F GQIDHQGICDEVNTFMFEGYDTTSTCLIFTLLMLALHEDVQKKCYEEIKYLPDDSDDISV *F FQFNELVYMECVIKESLRLFPSVPFIGRRCVEEGVVNGLIMPKNTQINIHLYEIMRDARH *F FSNPKMFQPDRFFPENTVNRHPFAFVPFSAGQRNCIGQKFAILEIKVLLAAVIRNFKILP *F VTLLDDLTFENGIVLRTKQNIKVKLVHRENK *F >4ac3 AC017771 comp(20241-21996) *F MWIALLGSSLLIGALWLLLRQLNKTYFILSLCKRVRTADGSPLESKVFVVPGKTRFGNNL *F DLLNLTPANIFSYIRESTAKANGQNYIWNFLFAPEYNIVRAEDAEEIFQSTKITTKNMSY *F ELIRPFLGDGLLISIDQKWHTRRKTLTPAFHFNILQSFLSIFKEESKKFIKILDKNVGFE *F LELNQIIPQFTLNNICETALGVKLDDMSEGNEYRKAIHDFEIVFNQRMCNPLMFFNWYFF *F LFGDYKKYSRILRTIHGFSSGIIQRKRQQFKQKQLGQVDEFGKKQRYAMLDTLLAAEAEG *F KIDHQGICDEVNTFMFGGYDTTSTSLIFTLLLLALHADVQERCYEELQDLPEDIDEVSMF *F QFNELIHLECVIKESLRLFPSAPIIGRTCIEESVMNGLVLPKNAQISIHIYDIMRDARHF *F PKPNQFLPERFLPENSVNRHPFAFVPFSAGPRNCIGQKFGVLEIKVLLAAVIRNFKLLPA *F TQLEDLTFENGIVLRTQQNIKVKFEARVK *F >4ad1 AC020402 comp(36565-39829) *F MFLIAIAIILATILVFKGVRIFNYIDHMAGIMEMIPGPTPYPFVGNLFQFGLKPAEYPKK *F VLQYCRKYDFQGFRSLVFLQYHMMLSDPAEIQNILSSSSLLYKEHLYSFLRPWLGDGLLT *F SSGARWLKHQKLYAPAFERSAIEGYLRVVHRTGGQFVQKLDVLSDTQEVFDAQELVAKCT *F LDIVCENATGQDSSSLNGETSDLHGAIKDLCDVVQERTFSIVKRFDALFRLTSYYMKQRR *F ALSLLRSELNRIISQRRHQLAAENTCQQGQPINKPFLDVLLTAKLDGKVLKEREIIEEVS *F TFIFTGHDPIAAAISFTLYTLSRHSEIQQKAAEEQRRIFGENFAGEADLARLDQMHYLEL *F IIRETLRLYPSVPLIARTNRNPIDINGTKVAKCTTVIMCLIAMGYNEKYFDDPCTFRPER *F FENPTGNVGIEAFKSVPFSAGPRRCIAEKFAMYQMKALLSQLLRRFEILPAVDGLPPGIN *F DHSREDCVPQSEYDPVLNIRVTLKSENGIQIRLRKR *F >4ae1 old 4e4 AL009194 16210-17867 *F MLVVLLVALLVTRLVASLFRLALKELRHPLQGVVPSVSRVPLLGAAWQMRSFQPDNLHDK *F FAEYVKRFGRSFMGTVLGHVVMVTAEPRHIDALLQGQHQLKKGTMYFALRGWLGDGLLLS *F RGKEWHTMRKIITPTFHFSILEQFVEVFDRQSSILVERLRTLSYGNEVVNIYPLVGLAAL *F DIITETAMGVNVDAQGADSEVVHAVKDLTNILATRFMRPHLLFPHLFRLCWPSGFRKQQA *F GVICLHEFTNGIIEQRRRLLAREANQDKPTKPHALLDTLLRATVDGQPLTDKQIRDEVNT *F FIFEGHDTTTSAVSFCLYLLSRHEAVQQKLFEELRMHYGQDLFRGVILSDFATLPYLSCV *F VKESLRLYPPIPAVARCLEKDLVIDEGYIPVGTNVVVLLWQLLRDEAIFTDPLVFQPERH *F LGEEAPRLSPYSYIPFSAGPRNCIGQKFALLEMKTMVTKVIRHYQLLPMGADVEPSIKIV *F LRSKSGVNVGLRPRLY *F >6a2 AC007549 comp(100072-101661) *F MFVLIYLLIAISSLLAYLYHRNFNYWNRRGVPHDAPHPLYGNMVGFRKNRVMHDFFYDYY *F NKYRKSGFPFVGFYFLHKPAAFIVDTQLAKNILIKDFSNFADRGQFHNGRDDPLTQHLFN *F LDGKKWKDMRQRLTPTFTSGKMKFMFPTVIKVSEEFVKVITEQVPAAQNGAVLEIKELMA *F RFTTDVIGTCAFGIECNTLRTPVSDFRTMGQKVFTDMRHGKLLTMFVFSFPKLASRLRMR *F MMPEDVHQFFMRLVNDTIALRERENFKRNDFMNLLIELKQKGRVTLDNGEVIEGMDIGEL *F AAQVFVFYVAGFETSSSTMSYCLYELAQNQDIQDRLRNEIQTVLEEQEGQLTYESIKAMT *F YLNQVISETLRLYTLVPHLERKALNDYVVPGHEKLVIEKGTQVIIPACAYHRDEDLYPNP *F ETFDPERFSPEKVAARESVEWLPFGDGPRNCIGMRFGQMQARIGLAQIISRFRVSVCDTT *F EIPLKYSPMSIVLGTVGGIYLRVERI *F >6a8 AC020447 comp(122688-124264) *F MALTYILFQVAVALLAILTYYIHRKLTYFKRRGIPFVAPHLIRGNMEELQKTKNIHEIFQ *F DHYNKFRESKAPFVGFFFFQSPAAFVIDLELAKQILIKDFSNFSNKGIFYNEKDDPISAH *F LFNLDGAQWRLLRNKLSSTFTSGKMKLMYPTVVSVANEFMTVMHEKVPKNSVLEIRDLVA *F RFTVDVIGTCAFGIQCNSLRDEKAEFLYFGKRSLVDKRHGTLLNGFMRSYPKLARKLGMV *F RTAPHIQEFYSRIVTETVAVREKEHIKRNDFMDMLIELKNQKEMTLENGDVVRGLTMEEV *F LAQAFVFFIAGFETSSSTMGFALYELAKNPDIQDKVRAEVEEVIEQHDQNFTYECTKDLK *F YLNQVLDETLRLYTIVPNLDRMAAKRYVVPGHPNFVIEAGQSVIIPSSAIHHDPSIYPEP *F FEFRPERFSPEESAGRPSVAWLPFGDGPRNCIGLRFGQMQARIGLALLIRNFKFSTCSKT *F PNPLVYDPKSFVLGVKDGIYLKVETV *F >6a9 AC020447 comp(115043-116610) *F MGVYSVLLAIVVVLVGYLLLKWRRALHYWQNLDIPCEEPHILMGSLTGVQTSRSFSAIWM *F DYYNKFRGTGPFAGFYWFQRPGILVLDISLAKLILIKEFNKFTDRGFYHNTEDDPLSGQL *F FLLDGQKWKSMRSKLSYTFTSGKMKYMFPTVVKVGHEFIEVFGQAMEKSPIVEVRDILAR *F FTTDVIGTCAFGIECSSLKDPEAEFRVMGRRAIFEQRHGPIGIAFINSFQNLARRLHMKI *F TLEEAEHFFLRIVRETVAFREKNNIRRNDFMDQLIDLKNSPLTKSESGESVNLTIEEMAA *F QAFVFFGAGFETSSTTMGFALYELAQHQDIQDRVRKECQEVIGKYNGEITYESMKDMVYL *F DQVISETLRLYTVLPVLNRECLEDYEVPGHPKYVIKKGMPVLIPCGAMHRDEKLYANPNT *F FNPDNFSPERVKERDSVEWLPFGDGPRNCIGMRFGQMQARSGLALLINRFKFSVCEQTTI *F PIVYSKKTFLISSETGIFLKVERV *F >6a13 AC020451 3757-5238 also AC007085 126189-124714 no introns *F MLTLLVLVFTVGLLLYVKLRWHYSYWSRRGVAGERPVYFRGNMSGLGRDLHWTDINLRIY *F RKFRGVERYCGYFTFMTKSLFIMDLELIRDIMIRDFSSFADRGLFHNVRDDPLTGNLLFL *F DGPEWRWLRQNLTQVFTSGKMKFMFPNMVEVGEKLTQACRLQVGEIEAKDLCARFTTDVI *F GSCAFGLECNSLQDPESQFRRMGRSVTQEPLHSVLVQAFMFAQPELARKLRFRLFRPEVS *F EFFLDTVRQTLDYRRRENIHRNDLIQLLMELGEEGVKDALSFEQIAAQALVFFLAGFDTS *F STTMSFCLYELALNPDVQERLRVEVLAVLKRNNQKLTYDSVQEMPYLDQVVAETLRKYPI *F LPHLLRRSTKEYQIPNSNLILEPGSKIIIPVHSIHHDPELYPDPEKFDPSRFEPEEIKAR *F HPFAYLPFGEGPRNCIGERFGKLQVKVGLVYLLRDFKFSRSEKTQIPLKFSSRNFLISTQ *F EGVHLRMEGLERP *F >6a14 AC007085 33266-34850 frameshift at amino acid 73 possible pseudogene *F MLFTIALVGVVLGLAYSLHIKIFSYWKRKGVPHETPLPIVGNMRGIVKKYHFRDINQRIY *F KKFKGQGPIAGMYTFFKRTALITDLDFIKQVMIKDFSYFQDRGAFTNPRDDPLTGHLFAL *F EGEEWRAMRHKLTPVFTSGKIKQMSKVIVDVGLRLGDAMDKAVKEAKVEEGNVEIKDLCA *F RFTTDVIGSCAFGLECNSLQDPSAEFRQKGREIFTRRRHSTLVQSFIFTNARLARKLRIK *F VLPDDLTQFFMSTVKNTVDYRLKNGIKRNDFIEQMIELRAEDQEAAKKGQGIDLSHGLTL *F EQMAAQAFVFFVAGFETSSSTMSLCLYELALQPDIQQRLREEIESVLANVDGGELNYDVL *F AQMTYLDQVLSETLRKHPLLPHLIRETTKDYQIPNSDIVLDKGILALIPVHNIHHDPEIY *F PEPEKFDPSRFDPEEVKNRHPMAYLPFGDGPRNCIGLRFGKIQAKIGLVSLLRRFKFSVS *F NRTDVPLIFSKKSFLLTTNDGIYLKVERV *F >6a15p this is a pseudogene on AC020451 2595-3373 with in frame *F stops and frame shifts *F AFTVTNSKLAKKLKMKILRDDLTDFFLSVVKPALSGMTLWTSPPSGGRSSRQGGSKFDLS *F HNWTLEQMAAQAIVFFLAGFETSSSTMSSCKYELALQPEI*NQIRDEIERVLEGNAITYD *F ALAKINYPEQVLSETLRKHPIQLIKFLLETQESFRVRNTELIVEKGTSLLIPVHSVHYDP *F HLYPHPKLFDSSRLKAYKSNSRHPFAYLPFGTFGPRSCIGLRFGKMQAKIGIVSLCQRFK *F FGDSDLTDIPL *F >6a16 AC004721 comp(2956-5070) *F MDFTLLLLTSLLSFLLGYLRYRFTYWELRGIPQLRPHFLFGHFFRLQSVHYSELLQETYD *F AFRGSAKVAGTYVFLRPMAVVLDLDLVKAVLIRDFNNFVDRRSFHGDPLTANLFNLQGEE *F WRNLRTKLSPTFTSGKMKYMFGTVSTVAQQLGGTFDELVGSQGAVLELHDLMARYTTDVI *F GSCAFGTECSSLREPQAEFRQVGRRIFRNSNRSIRWRIFKMTYLSSLAKLGLPVRILHPD *F ITKFFNRIVRETVELRERENIRRNDFMDLLLDLRRKGLTMEQMAAQAFVFFVAGFETSSS *F NMSYALFELAKNQDVQQKLRMEINDSIGKHGKLTYEAMMEMPYLDQTITETLRKYPALSS *F LTRLASEDYEIPSPDGGDPVVLEKGTSVHIPVLAIHYDPEVYPEPHEFRPERFAPDACRE *F RHPTAFLGFGDGPRNCIGLRFGRMQVKVGLITLLRRFRFSLPPGSPTQLKVTKRNLILLP *F SDGVRLQVDPVESRLM *F >6a17 AC020447 109264-110826 *F MLLLALIVVILSLLVFAARRRHGYWQRRGIPHDEVHPLFGNIKDWPNKRHIAEIFRDYYF *F KYKNSDYPFAGFFFFFTRTAVVTDMELLKRVLIKDFNHFENRGVFYNEIDDPLSATLFSI *F EGQKWRHLRHKLTPTFTSGKMKNMFPIVVKVGEEMDKVFRSKTAADRGQVLEVVDLVARY *F TADVIGNCAFGLNCNSLYDPKAEFVSIGKRAITEHRYGNMLDIFLFGFPKLSRRLRLKLN *F IQEAEDFYTKIVRETIDYRLRTKEKRNDFMDSLIEMYKNEQSGNSEDGLTFNELLAQAFI *F FFVAGFETSSTTMGFALYELARNQDVQDKLREEIGNVFGKHNKEFTYEGIKEMKYLEQVV *F METLRKYPVLAHLTRMTDTDFSPEDPKYFIAKGTIVVIPALGIHYDPDIYPEPEIFKPER *F FTDEEIAARPSCTWLPFGEGPRNCIGLRFGMMQTCVGLAYLIRGYKFSVSPETQIPMKIV *F VKNILISAENGIHLKVEKLAK *F >6a18 AC008285 85740-87319 *F MQLTYFLFQVAVALLAIVTYILHRKLTYFKRRGIPYDKPHPLRGNMEGYKKTRTVHEIHQ *F EYYNKYRNSKAPFVGFYLFQKPAAFVIDLELAKQILIKNFSNFTDKGIYYNEKDDPMSAH *F LFNLDGPQWRLLRSKLSSTFTSGKMKFMYPTVVSVAEEFMAVMHEKVSENSILDVRDLVA *F RFTVDVIGTCAFGIKCNSLRDEKAEFLHFGRRALLDSRHGNLVSGLMRSYPNLARRLGLC *F RNTAQIQEFYQRIVKETVTLREKENIKRNDFMDMLIGLKNQKNMTLENGEVVKGLTMDEI *F VAQAFVFFIAGFDTSSSTMGFALYELAKNPSIQDKVRAELGQVLEQHDQKFTYECIKDLK *F YLDQVINETLRHYTIVPNVDRVAAKRFVVPGNPKFVIEAGQSVIIPSSAIHHDPSIYPEP *F NEFRPERFSPEESAKRPSVAWLPFGEGPRNCIGLRFGQMQARIGLAMLIKNFTFSPCSAT *F PDPLTFDPHSAILLGIKGGIQLKVEAI *F >6a19 AC020447 113084-114646 *F MAILLGLVVGVLTLVAWWVLQNYTYWKRRGIPHDPPNIPLGNTGELWRTMPLAGILKRTY *F LKFRKQTDGPFAGFYLYAMKYIVITDVDFVKTVLIRDFDKFHDRGVYHNEKDDPLTNNLA *F TIEGQKWKNLRQKLTHTFTSAKMKSMFSTVLNVGDEMIRVVDEKISSSSQTLEVTDIVSR *F FTSDVIGICAFGLKCNSLRDPKAEFVQMGYSALRERRHGWLVDLLIFGMPKLAVKLGFQF *F LLPSVQKFYMKIVQDTIDYRMKRKVTRNDFMDTLIDMKQQYDKGDKENGLAFNEVAAQAF *F VFFLAGFEAGSTTMGFTLYELACNQDVQDKLRAEIDSVLERYNGKLEYDSMQDLFYMEKV *F INESLRKHPVVAHLARIATKPYQHSNPKYFIEAGTGVLVSTLGIHHDPEFYPEPEKFIPE *F RFDEEQVKKRPTCAFLPFGAGPRNCIGLRFGRMQVIIGLALLIHNFRFELHPKTPVPMKY *F TINNLLLGSEGGIHLNITKVVRD *F >6a20 AC020447 117416-119045 this seq has a frame shift at NKLR-NKTD *F MAVMIVLLIGVITFVAWYVHQHFNYWKRRGIPHDEPKIPYGNTSELMKTVHFADIFKRTY *F NKLRNKTDGPFVGFYMYFKRMVVVTDIDFAKTVLIREFDKFHDRGVFHNERDDPLSANLV *F NIDGQKWKTLRQKLTPTFTSGKMKTMFPTILTVGDELIRVFGETASADSDSMEITNVVAR *F FTADVIGSCAFGLDCHSLSDPKAKFVQMGTTAITERRHGKSMDLLLFGAPELAAKLRMKA *F TVQEVEDFYMNIIRDTVDYRVKNNVKRHDFVDMLIEMKLKFDNGDKENGLTFNEIAAQAF *F IFFLAGFETSSTTMGFALYELACHQDIQDKLRTEINTVLKQHNGKLDYDSMREMTYLEKV *F IDETMRKRPVVGHLIRVATQHYQHTNPKYNIEKGTGVIVPTLAIHHDPEFYPEPEKFIPE *F RFDEDQVQQRPACTFLPFGDGPRNCIGLRFGRMQVIVGMALLIHNFKFEFHPTKTVVPLE *F YRTDDFLLSSKGGIHLKVTRV *F >6a21 AC020447 comp(120585-122149) *F MSVGTVLLTALLALVGYLLMKWRSTMRHWQDLGIPCEEPHILMGSMKGVRTARSFNEIWT *F SYYNKFRGSGPFAGFYWFRRPAVFVLETSLAKQILIKEFNKFTDRGFFHNPEDDPLSGQL *F FLLDGQKWRTMRNKLSSTFTSGKMKYMFPTVVKVANEFTDVFGQNVAKSPVVEVRELLAR *F FTTDVIGTCAFGIECSSLKDPDAEFREMGRRSLTEQRLGPVGIGFVNSFPNLARRLHMKM *F TAEPIERFFMRIVRETVAFREQNNIRRNDFMDQLIDLKNKPLMVSQSGESVNLTIEEIAA *F QAFVFFAAGFETSSTTMGFALYELAQNQDIQNRVRKECQEVIEKCNGELNYESMKDLVYL *F DQVVSETLRLYTVLPVLNRECLEDYEVPGHPKYVIKKGMPVLIPCGAMHRDEKLYANPNT *F FNPDNFSPERVKERDSVEWLPFGDGPRNCIGMRFGQMQARIGLALLIKDFKFSVCEKTTI *F PMTYNKEMFLIASNSGIYLKAERV *F >6a22 AC020447 106987-108653 *F MLDVVALLLIALAVGFWFVRTRYSYWTRRGIGSEPARFPVGNMEGFRKNKHFIDIVTPIY *F EKFKGNGAPFAGFFMMLRPVVLVTDLELAKQILIQDFANFEDRGMYHNERDDPLTGHLFR *F IDGPKWRPLRQKMSPTFTSAKMKYMFPTVCEVGEELTQVCGELADNAMCGILEIGDLMAR *F YTSDVIGRCAFGVECNGLRNPEAEFAIMGRRAFSERRHCKLVDGFIESFPEVARFLRMRQ *F IHQDITDFYVGIVRETVKQREEQGIVRSDFMNLLIEMKQRGELTIEEMAAQAFIFFAAGF *F DTSASTLGFALYELAKQPALQAKLREEIDQALRLHNGEFTYDSMQELRYMELVIAETLRK *F YPILPQLTRISRHLYAAKGDRHFYIEPGQMLLIPVYGIHHDPALYPEPHKFIPERFLADQ *F LAQRPTAAWLPFGDGPRNCIGMRFGKMQTTIGLVSLLRNFHFSVCPRTDPKIEFLKSNIL *F LCPANGIYLKVQQLSQMSS *F >6a23 AC020447 111145-112718 *F MSLLLTLIALLVSLLLFMARRRHGYWQRRGIPHDVPHPIYGNMKDWPKKRHIAMIFRDYY *F TKYKRSVYPFAGFYFFFTRSAVITDLELVKRVLIKDFNHFENRGIFYNEIDDPLSATLFS *F IEGQKWRHLRHKLTPTFTSGKMKNMFPIIVKVGEEMEKIFSAKTTTGEGQVLEIVDLVAR *F YTADVIGNCAFGLNCNSLQNPNAEFVTIGKRAIIERRYGGLLDFLIFGFPKLSRRLRLKL *F NVQDVEDFYTSIVRNTIDYRLRTNEKRHDFMDSLIEMYEKEQAGNTEDGLSFNEILAQAF *F IFFVAGFETSSTTMGFALYELALDQDIQDQLRAEINNVLSKHNNEFTYEGIKEMKYLEQV *F VMETLRKYPVLAHLTRMTQTDFSPEDPKYFIAKGTTVVIPALGIHYDPEIYPEPEKFKPE *F RFTDEAIAARPSCTWLPFGEGPRNCIGLRFGLMQACVGLAYLIRGYKFSVSTETQIPMKF *F VVKSILLSAENGIHLKVEKLSK *F >6d2 AC020206 comp(66328-68065) also AC008350 *F MWTILLTILIAGLLYRYVKRHYTHWQRLGVDEEPAKIPFGVMDTVMKQERSLGMALADIY *F ARHEGKIVGIYMLNKRSILIRDAQLARQIMTSDFASFHDRGVYVDEDKDPLSANLFNLRG *F ASWRNLRQKLTPSFSSGKIKGMFGTIDDVGDKLVQHLEGALDQSDEVEIKDVMTTYAVDI *F IGSVIFGLEIDSFRNPKNEFREISSSTSRDESLLLKIHNMSMFICPPIAKLMNRLGYESR *F ILTSLRDMMKRTIEFREEHNVVRKDMLQLLIRLRNTGKIGEDDDQVWDMETAQEQLKSMS *F IEKIAAQAFLFYVAGSESTAAASAFTLYELSMYPELLKEAQEEVDAVLMKHNLKPKDRFT *F YEAVQDLKFLDICIMETIRKYPGLPFLNRECTEDYPVPGTNHIIAKGTPILISLFGMQRD *F PVYFPNPNGYDPHRFDSNNMNYDQAAYMPFGEGPRHCIALRMGKVNSKVAVAKILANFDL *F VQSPRKEVEFRFDAAPVLVTKEPLKLRLTKRK *F >6d4 AC017250 37993-39657 also AC008197 AC009846 *F MFSLILLAVTLLTLAWFYLKRHYEYWERRGFPFEKHSGIPFGCLDSVWRQEKSMGLAIYD *F VYVKSKERVLGIYLLFRPAVLIRDADLARRVLAQDFASFHDRGVYVDEERDPLSANIFSL *F RGQSWRSMRHMLSPCFTSGKLKSMFSTSEDIGDKMVAHLQKELPEEGFKEVDIKKVMQNY *F AIDIIASTIFGLDVNSFENPDNKFRKLVSLARANNRFNAMFGMMIFLVPSIAQFLFRIGF *F KNPVGLAMLQIVKETVEYREKHGIVRKDLLQLLIQLRNTGKIDENDEKSFSIQKTPDGHI *F KTISLEAITAQAFIFYIAGQETTGSTAAFTIYELAQYPELLKRLQDEVDETLAKNDGKIT *F YDSLNKMEFLDLCVQETIRKYPGLPILNRECTQDYTVPDTNHVIPKGTPVVISLYGIHHD *F AEYFPDPETYDPERFSEESRNYNPTAFMPFGEGPRICIAQRMGRINSKLAIIKILQNFNV *F EVMSRSEIEFENSGIALIPKHGVRVRLSKRVPKLS *F >6d5 AC018176 comp(132649-134791) *F MIGIYLLIAAVTLLYVYLKWTFSYWDRKGFPSTGVSIPFGALESVTKGKRSFGMAIYDMY *F KSTKEPVIGLYLTLRPALLVRDAQLAHDVLVKDFASFHDRGVYVDEKNDPMSASLFQMEG *F ASWRALRNKLTPSFTSGKLKAMFETSDSVGDKLVDSIRKQLPANGAKELELKKLMATYAI *F DIIATTIFGLDVDSFADPNNEFQIISKKVNRNNIEDIIRGTSSFLYPGLEKFFVKIGWKQ *F EATERMRELSNRTVDLREQNNIVRKDLLQLLLQLRNQGKINTDDNIWSAESTKNGVKSMS *F KDLIAGQLFLFYVAGYETTASTTSFTLYELTQNPEVMEKAKEDVRSAIEKHGGKLTYDAI *F SDMKYLEACILETARKYPALPLLNRICTKDYPVPDSKLVIQKGTPIIISLIGMHRDEEYF *F PDPLAYKPERYLENGKDYTQAAYLPFGEGPRMCIGARMGKVNVKIAIAKVLSNFDLEIRK *F EKCEIEFGVYGIPLMPKSGVPVRLSLKK *F >6g1 AC015208 comp(21239-22935) also AC007440 *F MVLTEVLFVVVAALVALYTWFQRNHSYWQRKGIPYIPPTPIIGNTKVVFKMENSFGMHLS *F EIYNDPRLKDEAVVGIYSMNKPGLIIRDIELIKSILIKDFNRFHNRYARCDPHGDPLGYN *F NLFFVRDAHWKGIRTKLTPVFTSGKVKQMYTLMQEIGKDLELALQRRGEKNSGSFITEIK *F EICAQFSTDSIATIAFGIRANSLENPNAEFRNYGRKMFTFTVARAKDFFVAFFLPKLVSL *F MRIQFFTADFSHFMRSTIGHVMEERERSGLLRNDLIDVLVSLRKEAAAEPSKPHYAKNQD *F FLVAQAGVFFTAGFETSSSTMSFALYEMAKHPEMQKRLRDEINEALVEGGGSLSYEKIQS *F LEYLAMVVDEVLRMYPVLPFLDREYESVEGQPDLSLKPFYDYTLENGTPVFIPIYALHHD *F PKYWTNPSQFDPERFSPANRKNIVAMAYQPFGSGPHNCIGSRIGLLQSKLGLVSLLKNHS *F VRNCEATMKDMKFDPKGFVLQADGGIHLEIVNDRLYDQSAPSLQ *F >6g2 AC015208 19014-20624 *F MELVLLILVASLIGIAFLALQQHYSYWRRMGVREIRPKWIVGNLMGLLNMRMSPAEFISQ *F LYNHPDAENEPFVGIHVFHKPALLLRDPEMVRNILVKDFAGFSNRYSSSDPKGDPLGSQN *F IFFLKNPAWKEVRLKLSPFFTGNRLKQMFPLIEEVGASLDAHLRQQPLHNERMRCFDLEA *F KELCALYTTDVIATVAYGVSANSFTDPKCEFRRHGRSVFEFNLLRAAEFTLVFFLPHLVP *F FVRFKVVPAEATRFLRKTINYVMSEREKSGQKRNDLIDILIEFRRSTQLAKASGIKDQFV *F FEGDILVAQAVLFFTAGFESSSSTMAFAMYELAKDTDVQQRLREEIKDALVESGGQVTLK *F MIESLEFMQMILLEVLRMYPPLPFLDRECTSGRDYSLAPFHKKFVVPKGMPVYIPCYALH *F MDPQYFPQPRKFLPERFSPENRKLHTPYTYMPFGLGPHGCIGERFGYLQAKVGLVNLLRN *F HMITTSERTPHRMQLDPKAIITQAKGGIHLRLVRDALGV *F >6t1 AC012831 comp(42975-44564) no introns *F MIAVFSLIAAALAVGSLVLLPVVLRGGCLLVVTIVWLWQILHFWHWRRLGVPFVPAAPFV *F GNVWNLLRGACCFGDQFRELYESKEAAGRAFVGIDVLHNHALLLRDPALIKRIMVEDFAQ *F FSSRFETTDPTCDTMGSQNLFFSKYETWRETHKIFAPFFAAGKVRNMYGLLENIGQKLEE *F HMEQKLSGRDSMELEVKQLCALFTTDIIASLAFGIEAHSLQNPEAEFRRMCIEVNDPRPK *F RLLHLFTMFFFPRLSHRVGTHLYSEEYERFMRKSMDYVLSQRAESGENRHDLIDIFLQLK *F RTEPAESIIHRPDFFAAQAAFLLLAGFDTSSSTITFALYELAKNTTIQDRLRTELRAALQ *F SSQDRQLSCDTVTGLVYLRQVVDEVLRLYPPTAFLDRCCNSRTGYDLSPWNGGSPFKLRA *F GTPVYISVLGIHRDAQYWPNPEVFDPERFSAEQRQQHHPMTYLPFGAGPRGCIGTLLGQL *F EIKVGLLHILNHFRVEVCERTLPEMRFDPKAFVLTAHNGTYLRFVKNSL *F >6t2p AC014742 1042-2504 *F MVIAFFIFL*CAALAVGSVVLLPLIALLAVWLWQRRHFRIWRRLGVPYLPAAPVLGNVLN *F VETAACCFGDQFRELYERKEAAGRAIVGINVLHSHALLLRDPALIRRILVEDFPEFSSSF *F KSTDAIRDTMGSGNLLFTKYKTWWETHKIFAIRLGGRRIRSLLYGLLERI*QNLEAHMAQ *F KLNGAESVELEVKQLCALFTTDIFAKFALQSLQNPEAEFRPMCIEVNDPKPKRLSHHLFT *F GFTPPIYRVRTHLYSEEYERFMRKSMNYVLAQRAEN*EKRYDLIDMFLQMHRTETAEGII *F HRPDFYVAQAAFLLLAGFDTSSSFALYELAKNPTIEHRLQAELRVDLQSSHNHQLSYDTL *F TGLVYLRQVLEDLPFGAGPRGCIGTLLGQLGIKVGLLHTLKHFRVELCERTLPEMRFDP* *F ASVLTAHNGTFLRFVRNSL* *F >6t3 AC015208 comp(17123-18689) *F MLLIWLLLLTIVTLNFWLRHKYDYFRSRGIPHLPPSSWSPMGNLGQLLFLRISFGDLFRQ *F LYADPRNGQAKIVGFFIFQTPALMVRDPELIRQVLIKNFNNFLNRFESADAGDPMGALTL *F PLAKYHHWKESRQCMSQLFTSGRMRDVMYSQMLDVASDLEQYLNRKLGDRLERVLPLGRM *F CQLYTTDVTGNLFYSLNVGGLRRGRSELITKTKELFNTNPRKVLDFMSVFFLPKWTGVLK *F PKVFTEDYARYMRHLVDDHHEPTKGDLINQLQHFQLSRSSNHYSQHPDFVASQAGIILLA *F GFETSSALMGFTLYELAKAPDIQERLRSELREAFISTATLSYDTLMTLPYLKMVCLEALR *F LYPAAAFVNRECTSSASEGFSLQPHVDFIVPPGMPAYISILGLHRDERFWPEPCVFDPER *F FGPERSRHIHPMTYIPFGAGPHGCIGSRLGVLQLKLGIVHILKQYWVETCERTVSEIRFN *F PKSFMLESENEIYLRFCRSSL *F >6u1 AC017706 15959-17471 also AC008288 *F MHRTLLTALGELSVVYALVKFSLGYWKRRGILHEKPKFLWGNIKGVVSGKRHAQDALQDI *F YTAYKGRAPFVGFYACLKPFILALDLKLVHQIIFTDAGHFTSRGLYSNPSGEPLSHNLLQ *F LDGHKWRSLHAKSAEVFTPANMQKLLVRLSQISSRIQRDLGEKSLQTINISELVGAYNTD *F VMASMAFGLVGQDNVEFAKWTRNYWADFRMWQAYLALEFPLIARLLQYKSYAEPATAYFQ *F KVALSQLQLHRRRDRQPLQTFLQLYSNAEKPLTDIEIAGQAFGFVLAGLGPLNATLAFCL *F YELARQPEVQDRTRLEINKALEEHGGQVTPECLRELRYTKQVLNETLRLHTPHPFLLRRA *F TKEFEVPGSVFVIAKGNNVLIPTAAIHMDPGIYENPQRFYPERFEEQARRSRPAAAFLPF *F GDGLRGCIAARFAEQQLLVGLVALLRQHRYAPSAETSIPVEYDNRRLLLMPKSDIKLSVE *F RVDKL *F >6v1 AC011761 comp(54926-57766) also AC015002 *F MVYSTNILLAIVTILTGVFIWSRRTYVYWQRRRVKFVQPTHLLGNLSRVLRLEESFALQL *F RRFYFDERFRNEPVVGIYLFHQPALLIRDLQLVRTVLVEDFVSFSNRFAKCDGRSDKMGA *F LSLFLAKQPEWREIRTRLAPAFAGAKLKQMFSLMEEIGCDLEWYLKRLTRDLRRGDAERG *F AIVSIKDVCDLYNTDMIASIAFGLRSYSLRNTQSEIGSHCQDLFRPNVRRIIDLFVIFYL *F PKLVPLLRPKLFTEPHAEFLRRVIQLVIEERERGGDLRNDLIEMLLTLKKEADLQQDKSH *F FTHHRDFLAAQAASFEVAGIETCSASMSFALYELAKQPLMQSRLRREIREAFASNPNGRL *F TYEAVARMEFLDMVVEETLRKYPIVPLLERECTPINKKRFYSLRPHAECYTRRGMPVFIS *F NLAIHHDPKYWPDPDRFDPERFSAANKALQAPMSYMPFGAGPRNCIGMQIGLLQIKLGLV *F YFLHQHRVEICDRTVERIQFDAKFALLASEQRIYLKVDCL *F >6w1 AC014226 comp(15025-16689) also AC008257 *F MLLLLLLGSLTIVFYIWQRRTLSFWERHGVKYIRPFPVVGCTREFLTAKVPFFEQIQKFH *F EAPGFENEPFVGVYMTHRPALVIRDLELIKTVMIKKFQYFNNRVLQTDPHNDALGYKNLF *F FARSPGWRELRTKISPVFTSGKIKQMYPLMVKIGKNLQDSAERLGSGTEVQVKDLCSRFT *F TDLIATIAFGVEANALQDAKSEFFYHNRAIFSLTLSRGIDFAIIFMIPALASLARVKLFS *F RETTKFIRSSVNYVLKERERTGEKRNDLIDILLALKREAAANPGKMSKEVDLDYLVAQAA *F VFQTAGFETSASTMTMTLYELAKNEALQDRLRQEIVDFFGDEDHISYERIQEMPYLSQVV *F NETLRKYPIVGYIERECSQPAEGERFTLEPFHNMELPHGMSIYMSTVAVHRDPQYWPDPE *F KYDPERFNSSNRDNLNMDAYMPFGVGPRNCIGMRLGLLQSKLGLVHILRNHRFHTCDKTI *F KKIEWAPTSPVMASKRDIILRVEKVSGKKDFGQK *F >9b1 AC019916 10502-12238 *F MSFVEICLVLATIGLLLFKWSTGTFKAFEGRNLYFEKPYPFLGNMAASALQKASFQKQIS *F EFYNRTRHHKLVGLFNLRTPMIQINDPQLIKKICVKDFDHFPNHQTLNIPNERLVNDMLN *F VMRDQHWRNMRSVLTPVFTSAKMRNMFTLMNESFAQCLEHLKSSQPIAAGENAFELDMKV *F LCNKLSNDVIATTAFGLKVNSFDDPENEFHTIGKTLAFSRGLPFLKFMMCLLAPKVFNFF *F KLTIFDSTNVEYFVRLVVDAMQYREKHNITRPDMIQLLMEAKKESKDNWTDDEIVAQCFI *F FFFAAFENNSNLICTTAYELLRNLDIQERLYEEVKETQEALKGAPLTYDAAQEMTYMDMV *F ISESLRKWTLSAAADRLCAKDYTLTDDEGTKLFEFKAGDNINIPICGLHWDERFFPQPQR *F FDPERFSERRKKDLIPYTYLPFGVGPRSCIGNRYAVMQAKGMLYNLMLNYKIEASPRTTR *F DMWESARGFNIIPTTGFWMQLVSRK *F >9b2 AC019916 8190-9903 *F MALIEICLALVVIGYLIYKWSTATFKTFEERKLYFEKPYPFVGNMAAAALQKSSFQRQLT *F EFYERTRQHKLVGFFNMRTPMITLNDPELIKKVCVKDFDHFPNHQPFITSNDRLFNDMLS *F VMRDQRWKHMRNTLTPVFTAAKMRNMFTLMNESFAECLQHLDSSSKTLPGRKGFEVDMKV *F MCNKLSNDIIATTAFGLKVNSYDNPKNEFYEIGQSLVFSRGLQFFKFMLSTLVPKLFS *F LLKLTIFDSAKVDYFA RLVVEAMQYREKHNITRPDMIQLLMEAKNESEDKWTDDEIVAQCFI *F FFFAAFENNSNLICTTTYELLYNPDVQERLYEEIVETKKALNGAPLTYDAVQKMTYMDMV *F ISESLRKWTLAAATDRLCSKDYTLTDDDGTKLFDFKVGDRINIPISGLHLDDRYFPEPRK *F FDPDRFSEERKGDMVPYTYLPFGVGPRNCIGNRYALMQVKGMLFNLLLHYKIEASPRTIK *F DLWGSASGFNFTPRSGFWMHLVPRK *F >9c1 AC019937 comp(1757-3443) *F MVFVELSIFVAFIGLLLYKWSVYTFGYFSKRGVAHEKPIPLLGNIPWSVLMGKESYIKHS *F IDLHLRLKQHKVYGVFNLRDPLYYLSDPELIRQVGIKNFDTFTNHRKGITEGFNDTSVIS *F KSLLSLRDRRWKQMRSTLTPTFTSLKIRQMFELIHFCNVEAVDFVQRQLDAGTSELELKD *F FFTRYTNDVIATAAFGIQVNSFKDPNNEFFSIGQRISEFTFWGGLKVMLYILMPKLMK *F ALRVPVMDMNNVDYFK *F KLVFGAMKYRKEQSIVRPDMIHLLMEAQRQFKAEQEGSAESAAQQD *F KAEFNDDDLLAQCLLFFSAGFETVATCLSFTSYELMMNPEVQEKLLAEILAVKEQLGEKP *F LDYDTLMGMKYLNCVVSESLRKWPPAFIVDRMCGSDFQLKDEEGEVVVNLREDDLVHINV *F GALHHDPDNFPEPEQFRPERFDEEHKHEIRQFTYLPFGVGQRSCIGNRLALMEVKSLIFQ *F LVLRYHLKPTDRTPADMMSSISGFRLLPRELFWCKLESRGPA *F >9f2 AC017132 AC017240 AC009741 AC017170 AC007594 PLUS ESTs *F revised 9/11/2000 *F MLWEFFALFAIAAALFYRWASANNDFFKDRGIAYEKPVLYFGNMAGMFLRKRAMFDIVCD *F LYTKGGSKKFFGIFEQRQPLLMVRDPDLIKQITIKDFDHFINHRNVFATSSDDDPHDMSN *F LFGSSLFSMRDARWKDMRSTLSPAFTGSKMRQMFQLMNQVAKEAVDCLKQDDSRVQENEL *F DMKDYCTRFTNDVIASTAFGLQVNSFKDRENTFYQMGKKLTTFTFLQSMKFMLFFALKGL *F NKILKVELFDRKSTQYFVRLVLDAMKYRQEHNIVRPDMINMLMEARGIIQTEKTKASAVR *F EWSDRDIVAQCFVFFFAGFETSAVLMCFTAHELMENQDVQQRLYEEVQQVDQDLEGKELT *F YEAIMGMKYLDQVVNEVLRKWPAAIAVDRECNKDITFDVDGQKVEVKKGDVIWLPTCGFH *F RDPKYFENPMKFDPERFSDENKESIQPFTYFPFGLGQRNCIGSRFALLEAKAVIYYLLKD *F YRFAPAKKSCIPLELITSGFQLSPKGGFWIKLVQRN *F >9f3p AC017132 AC017240 AC009741 AC017170 AC007594 PLUS ESTs *F revised 9/11/2000 *F MLWEFLALFAIATALFYRWASANNDFFKDRGIAYEKPVLYLGNMAGMFLRKRAMLDIVCD *F LYTKGGSGKFFGIFAQRQPLLMVRDPDLIKQITIKDFDHFINHRNEFDTSSDDDPHDMSN *F LFSSSLFSMRDARWKDMRSTLSPAFTGSKMRQMFQLMNQVAKEAVDCLKQDDSRVQENEL *F DMKDYCTRFTNDVIASTAFGLQVNSFKDRENTFYQMGKKLTTFTFLQNMKFILLFALKSL *F NKILKVEIFDRKSTQYFVRLVLDAMKYRQEHNIVRPDMINMLMEARGIIQTEKTKASAVR *F EWSDRDIVAQCFVFFFAGFETSAVLMCFTAHELMENQDVQQRLYEEVQQVDQDLEGKELK *F YLDQVVSEVLRKWPPAIAFDRECNKEAKAVIYYLLKDYRFAPAKKSCIPLELISSGFQLS *F PKGGFWIKLVQRN *F >9h1 AC017648 8657-10342 also AC005450 *F MDQSMIALALFIILLVLLYKWSVAKYDVFSERGVSHEKPWPLIGNIPLKAMIGGMPVLKK *F MIELHTKHTGSPVYGIYALRDAVFFVRDPELIKLIGIKEFDHFVNHNSMHNNIQESILSK *F SLISLRDGRWKEMRNILTPAFTGSKMRIMYDLIQSCSEEGVIHIQEQLELSQDASIELEM *F KDYFTRFANDVIATVAFGISINSFRRKDNEFFRIGQAMSRISAWSVVKAMLYALFPRLMK *F VLRIQVLDTKNIDYFSSLVTAAMRYRQEHKVVRPDMIHLLMEAKQQRLADLSDKSKDELY *F YSEFTADDLLAQCLLFFFAGFEIISSSLCFLTHELCLNPTVQDRLYEEIISVHEELKGQP *F LTYDKLTKMKYLDMVVLEALRKWPPSISTDRECRQDIDLFDENGQKLFSARKGDVLQIPI *F FSLHHDPENFEDPEFFNPERFADGHALESRVYMPFGVGPRNCIGNRMALMELKSIVYQLL *F LNFKLLPAKRTSRDLLNDIRGHGLKPKNGFWLKFEARQ *F >12a4 AC006091 85973-87865 also AC015190 AC008141 rev 9/11/2000 *F EST AI109374 *F MLKVRSALSLIQSQKATLSLATQKRWQTNVATAEAREDSEW *F LQAKPFEQIPRLNMWALSMKMSMPGGKYKNMELMEMFEAMRQDYGDIFFMPGIMGNPPFL *F STHNPQDFEVVFRNEGVWPNRPGNYTLLYHREEYRKDFYQGVMGVIPTQGKPWGDFRTVV *F NPVLMQPKNVRLYYKKMSQVNQEFVQRILELRDPDTLEAPDDFIDTINRWTLESVSVVAL *F DKQLGLLKNSNKESEALKLFHYLDEFFIVSIDLEMKPSPWRYIKTPKLKRLMRALDGIQE *F VTLAYVDEAIERLDKEAKEGVVRPENEQSVLEKLLKVDRKVATVMAMDMLMAGVDTTSST *F FTALLLCLAKNPEKQARLREEVMKVLPNKNSEFTEASMKNVPYLRACIKESQRLHPLIVG *F NARVLARDAVLSGYRVPAGTYVNIVPLNALTRDEYFPQASEFLPERWLRSPKDSESKCPA *F NELKSTNPFVFLPFGFGPRMCVGKRIVEMELELGTARLIRNFNVEFNYPTENAFRSALIN *F LPNIPLKFKFIDLPN *F >12a5 AC006091 83449-85296 also AC015190 AC008141 *F MLKGRIALNILQSQKPIVFSASQQRWQTNVPTAEIRNDPEWLQAKPFEEIPKANILSLFA *F KSALPGGKYKNLEMMEMIDALRQDYGNIIFLPGMMGRDGLVMTHNPKDFEVVFRNEGVWP *F FRPGSDILRYHRTVYRKDFFDGVQGIIPSQGKSWGDFRSIVNPVLMQPKNVRLYFKKMSQ *F VNQEFVELIKEIRDASTQEVPGNFLETINRWTLESVSVVALDKQLGLLRESGKNSEATKL *F FKYLDEFFLHSADLEMKPSLWRYFKTPLLKKMLRTMDSVQEVTLKYVDEAIERLEKEAKE *F GVVRPEHEQSVLEKLLKVDKKVATVMAMDMLMAGVDTTSSTFTALLLCLAKNPEKQARLR *F EEVMKVLPNKDSEFTEASMKNVPYLRACIKESQRVYPLVIGNARGLTRDSVISGYRVPAG *F TIVSMIPINSLYSEEYFPKPTEFLPERWLRNASDSAGKCPANDLKTKNPFVFLPFGFGPR *F MCVGKRIVEMELELGTARLIRNFNVEFNHSTKNAFRSALINLPNIPLKFKFTDVPN *F >12b2 AC018326 7227-9141 also AC004345 AC004657 *F MWKYSNKIIYRNVSGNQLWFNRNSSVGGTLSQQVRSWQKEQELLKSRNLFTNNGYICSQT *F QLELADSRIDEKWQQARSFGEIPGPSLLRMLSFFMPGGALRNTNLIQMNRLMREMYGDIY *F CIPGMMGKPNAVFTYNPDDFEMTYRNEGVWPIRIGLESLNYYRKIHRPDVFKGVGGLASD *F QGQEWADIRNKVNPVLMKVQNVRQNLPQLDQISKEFIDKLETQRNPETHTLTTDFHNQLK *F MWAFESISFVALNTRMGLLSDNPDPNADRLAKHMRDFFNYSFQFDVQPSIWTFYKTAGFK *F KFLKTYDNITDITSNYIETAMRGFGKNDDGKTKCVLEQLLEHNKKVAVTMVMDMLMAGID *F TTSSACLTILYHLARNPSKQEKLRRELLRILPTTKDSLTDQNTKNMPYLRACIKEGLRIT *F SITPGNFRITPKDLVLSGYQVPRGTGVLMGVLELSNDDKYFAQSSEFIPERWLKSDLAPD *F IQACPAARTRNPFVYLPFGFGPRTCIGKRIAELEIETLLVRLLRSYKVSWLPETPIEYES *F TIILSPCGDIRFKLEPVGDLM *F >12c1 AC009385 comp(57935-59646) also AC012807 *F MLRLTVKHGLRANSQLAATRNPDASSYVQQLESEWEGAKPFTELPGPTRWQLFRGFQKGG *F EYHQLGMDDVMRLYKKQFGDICLIPGLFGMPSTVFTFNVETFEKVYRTEGQWPVRGGAEP *F VIHYRNKRKDEFFKNCMGLFGNGAEWGKNRSAVNPVLMQHRNVAIYLKPMQRVNRQFVNR *F IREIRDKESQEVPGDFMNTINHLTFESVATVALDRELGLLREANPPPEASKLFKNIEVLM *F DSFFDLGVRPSLYRYIPTPTYKKFSRAMDEIFDTCSMYVNQAIERIDRKSSQGDSNDHKS *F VLEQLLQIDRKLAVVMAMDMLMGGVDTTSTAISGILLNLAKNPEKQQRLREEVLSKLTSL *F HSEFTVEDMKSLPYLRAVIKESLRLYPVTFGNARSAGADVVLDGYRIPKGTKLLMTNSFL *F LKDDRLYPRAKEFIPERWLRRKDDDKSDVLMNKDLNAFIYLPFGFGPRMCVGKRIVDLEM *F ELTVANLVRNFHIEYNYSTEKPYKCRFLYKPNIPLKFKFTDLKY *F >12d1 AC008187 comp(84371-86114) *F MNTLSSARSVAIYVGPVRSSRSASVLAHEQAKSSITEEHKTYDEIPRPNKFKFMRAFMPG *F GEFQNASITEYTSAMRKRYGDIYVMPGMFGRKDWVTTFNTKDIEMVFRNEGIWPRRDGLD *F SIVYFREHVRPDVYGEVQGLVASQNEAWGKLRSAINPIFMQPRGLRMYYEPLSNINNEFI *F ERIKEIRDPKTLEVPEDFTDEISRLVFESLGLVAFDRQMGLIRKNRDNSDALTLFQTSRD *F IFRLTFKLDIQPSMWKIISTPTYRKMKRTLNDSLNVAQKMLKENQDALEKRRQAGEKINS *F NSMLERLMEIDPKVAVIMSLDILFAGVDATATLLSAVLLCLSKHPDKQAKLREELLSIMP *F TKDSLLNEENMKDMPYLRAVIKETLRYYPNGLGTMRTCQNDVILSGYRVPKGTTVLLGSN *F VLMKEATYYPRPDEFLPERWLRDPETGKKMQVSPFTFLPFGFGPRMCIGKRVVDLEMETT *F VAKLIRNFHVEFNRDASRPFKTMFVMEPAITFPFKFTDIEQ *F >12e1 AC018294 2070-3932 *F MLSTQWNANKQISRQIYQLCRGLAQKVRKIYFKYIFILNLEEAKPYADIPGPSKLQLIRA *F FLPGGLYKNLPVHEMFLDMNRQYGSIFRMPSVAGTDLVLTMNPQDYEVIFRNEGQYPYRR *F SFEVMDYFKRVHRREVFDGYDGLTSGNGPAWGKMRTAVNPILLQPRNAKLYMTNLVQVSD *F EFLERIRIIRDPVTQEMPDDFAVDIRHLVIESICSVALNTHLGLLGEQRNNKDIQKLVLA *F LQDVVELGFQLDIMPAFWKYLPMPNFKKLMRSLDTITDFCYFHIGNALKRIEEDAKAGTL *F NEIGLETSLLEKLARFDRQTAVIIAMDLLFAGADPTLVTLGGILFSLSKSPDKQARLLEE *F IRGILPNKDSSLTIENMRNLPYLRACIKEGIRMYPIGPGTLRRMPHDVVLSGYRVVAGTD *F VGIAANYQMANMEQFVPKVREFIPERWLRDESNSHLVGETATPFMYLPFGFGPRSCAGKR *F IVDMMLEIAISRLVRNFKIGFDYPIENAFKAQFFVQPNIPFKFKFIERNE *F >18a1 AC012164 114600-117669 also AC015216 *F MLADSYLIKFVLRQLQVQQDGDAQHLLMVFLGLLALVTLLQWLVRNYRELRKLPPGPWGL *F PVIGYLLFMGSEKHTRFMELAKQYGSLFSTRLGSQLTVVMSDYKMIRECFRREEFTGRPD *F TPFMQTLNGYGIINSTGKLWKDQRRFLHDKLRQFGMTYMGNGKQQMQKRIMTEVHEFIGH *F LHASDGQPVDMSPVISVAVSNVICSLMMSTRFSIDDPKFRRFNFLIEEGMRLFGEIHTVD *F YIPTMQCFPSISTAKNKIAQNRAEMQRFYQDVIDDHKRSFDPNNIRDLVDFYLCEIEKAK *F AEGTDAELFDGKNHEEQLVQVIIDLFSAGMETIKTTLLWINVFMLRNPKEMRRVQDELDQ *F VVGRHRLPTIEDLQYLPITESTILESMRRSSIVPLATTHSPTRDVELNGYTIPAGSHVIP *F LINSVHMDPNLWEKPEEFRPSRFIDTEGKVRKPEYFIPFGVGRRMCLGDVLARMELFLFF *F ASFMHCFDIALPEGQPLPSLKGNVGATITPESFKVCLKRRPLGPTAADPHHMRNVGAN *F >28a5 AC018242 6065-8082 also AC001660 *F MVLITLTLVSLVVGLLYAVLVWNYDYWRKRGVPGPKPKLLCGNYPNMFTMKRHAIYDLDD *F IYRQYKNKYDAVGIFGSRSPQLLVINPALARRVFVSNFKNFHDNEIAKNIDEKTDFIFAN *F NPFSLTGEKWKTRRADVTPGLTMGRIKTVYPVTNKVCQKLTEWVEKQLRLGSKDGIDAKH *F MSLCFTTEMVTDCVLGLGAESFSDKPTPIMSKINDLFNQPWTFVLFFILTSSFPSLSHLI *F KLRFVPVDVERFFVDLMGSAVETRRAQLAAGKQFERSDFLDYILQLGEKRNLDNRQLLAY *F SMTFLLDGFETTATVLAHILLNLGRNKEAQNLLREEIRSHLQDGTIAFEKLSDLPYLDAC *F VQETIRLFPPGFMSNKLCTESIEIPNKEGPNFVVEKGTTVVVPHYCFMLDEEFFPNPQSF *F QPERFLEPDAAKTFRERGVFMGFGDGPRVCIGMRFATVQIKAAIVELISKFNVKINDKTR *F KDNDYEPGQIITGLRGGIWLDLEKL* *F >28c1 AC014191 comp(8254-10002) also AL133495 *F MFGSLLLGIATLLGAIYAFLVSNFGHWRRRGVTEPRALPLFGSFPNMIWPRQHFTMDMRD *F IYMHYRNTHSYVGCYLLRAPKLLVLEPRLVYEIYVSAFSHFENNDASKMVDIAKDRLVAL *F NPFVLEGEEWRHQRAVFSTLLTNGRIRTTHAIMQRVCLDLCQFIAIKSAGGKDLDCIDLG *F LRFTGESLFDCVLGIQARTFTDNPLPVVRQNHEMSAENRGLAIAGAVHGLFPNLPRWLRP *F KVFPRSHDRFYGQMISEALRLRRSKHQERNDFINHLLEMQRELDLSEEDMASHAMTFMFD *F GLDTTSNSIAHCLLLLGRNPDCQRRLYEELQLVNPGGYLPDLDALIDLPYLSACFNESLR *F IYPAGGWASKTCTKEYELRGSHHSEPLKLRPGDHVMVPIYALHNDPDLYPEPDVFRPERF *F LDGGLKNCKQQGIFLGFGNGPRQCVGMRLGLAMAKAALAAIVQRFEVVVSPRTLNGTELD *F PLIFVGVHKGGIWLQFVPRKNVTTK *F >28d1 AC017780 comp(28385-30262) also AC009355 AC008324 AC008327 *F MCPISTALFVIAAILALIYVFLTWNFSYWKKRGIPTAKSWPFVGSFPSVFTQKRNVVYDI *F DEIYEQYKNTDSIVGVFQTRIPQLMVTTPEYAHKIYVSDFRSFHDNEMAKFTDSKTDPIL *F ANNPFVLTGEAWKERRAEVTPGLSANRVKAAYPVSLRVCKKFVEYIRRQSLMAPAQGLNA *F KDLCLCYTTEVISDCVLGISAQSFTDNPTPMVGMTKRVFEQSFGFIFYTVVANLWPPITK *F FYSVSLFAKDVAAFFYDLMQKCIQVRRESPAAQQRDDFLNYMLQLQEKKGLNAAELTSHT *F MTFLTDGFETTAQVLTHTLLFLARNPKEQMKLREEIGTAELTFEQISELPFTEACIHETL *F RIFSPVLAARKVVTEPCELTNKNGVSVKLRPGDVVIIPVNALHHDPQYYEEPQSFKPERF *F LNINGGAKKYRDQGLFFGFGDGPRICPGMRFSLTQIKAALVEIVRNFDIKVNPKTRKDNE *F IDDTYFMPALKGGVWLDFVERN *F >28d2 AC017780 comp(31545-33456) AC008324 revised 9/11/2000 *F MCPVTTFLVLVLTLLVLVYVFLTWNFNYWRKRGIKTAPTWPFVGSFPSIFTRKRNIAYDI *F DDIYEKYKDTDNMVGVFTTRVPQLLVMCPEYIHKIYATDFRSFHNNEWRNFVNKKTDM *F ILGNNPFVLTGDEWKERRSEIMPALSPNRVKAVYPVSQSVCKKFVEYIRRQQQMATSEGL *F DAMDLSLCYTTEVVSDCGLGVSAQSFTDTPTPLLKMIKRVFNTSFEFIFYSVVTNLWQKV *F RKFYSVPFFNKETEVFFLDIIRRCITLRLEKPEQQRDDFLNYMLQLQEKKGLHTDNILIN *F TMTFILDGFETTALVLAHIMLMLGRNPEEQDKVRKEIGSADLTFDQMSELPHLDACIYET *F LRLFSPQVAARKLVTEPFEFANKNGRTVHLKPGDVVTIPVKALHHDPQYYEDPLTFKPER *F FLESNGGGMKSYRDRGVYLAFGDGPRHCPGMRFALTQLKAALVEILRNFEIKVNPKTRSD *F NQIDDTFFMATLKGGIYLDFKDL *F >49a1 AC017930 48452-54739 AC007398 AC007418 ESTs AA941795 AI258819 AA697999 *F revised at intron 3 9/6/2000. 13 exons *F MQRLRTGESSNPKKLNVSQQPVTSVATTRTTASSLPAETTSSPAAAVRPYSEVPGPYPLP *F LIGNSWRFAPLIGTYKISDLDKVMNELHVNYGKMAKVGGLIGHPDLLFVFDGDEIRNIFK *F KEEAMPHRPSMPSLRHYKGDLRRDFFGDVAGLIGVHGPKWEAFRQEVQHILLQPQTAKKY *F IPPLNDIASEFMGRIELMRDEKDELPANFLHELYKWALESVGRVSLDTRLGCLSPEGSEE *F AQQIIEAINTFFWAVPELELRMPLWRIYPTKAYRSFVKALDQFTAICMKNIGKTMDKADA *F DEARGLSKSEADISIVERIVRKTGNRKLAAILALDLFLVGVDTTSVAASSTIYQLAKNPD *F KQKKLFDELQKVFPHREADINQNVLEQMPYLRACVKETLRMRPVVIANGRSLQSDAVING *F YHVPKGTHVIFPHLVVSNDPAYFPEPKRFLPERWLKQSTDAAGCPHANQKIHPFVSLPFG *F FGRRMCVGRRFAEIELHTLLAKIFRKYKVSYNSGEFVYRVNSTYIPQSPLNFKLTLRDE *F >301a1 AC017275 comp(35594-38192) also AC007356 *F MNNLSLKAWRSTVSCGPNLRQCVPRISGAGSRRAQCRESSTGVATCPHLADSEEASAPRI *F HSTSEWQNALPYNQIPGPKPIPILGNTWRLMPIIGQYTISDVAKISSLLHDRYGRIVRFG *F GLIGRPDLLFIYDADEIEKCYRSEGPTPFRPSMPSLVKYKSVVRKDFFGDLGGVVGVHGE *F PWREFRSRVQKPVLQLSTIRRYLQPLEVITEDFLVRCENLLDENQELPEDFDNEIHKWSL *F ECIGRVALDTRLGCLESNLKPDSEPQQIIDAAKYALRNVATLELKAPYWRYFPTPLWTRY *F VKNMNFFVGVCMKYIQSATERLKTQDPSLRAGEPSLVEKVILSQKDEKIATIMALDLILV *F GIDTISMAVCSMLYQLATRPVDQQKVHEELKRLLPDPNTPLTIPLLDQMHHLKGFIKEVF *F RMYSTVIGNGRTLMEDSVICGYQVPKGVQAVFPTIVTGNMEEYVTDAATFRPERWLKPQH *F GGTPGKLHPFASLPYGYGARMCLGRRFADLEMQILLAKLLRNYKLEYNHKPLDYAVTFMY *F APDGPLRFKMTRV *F >302a1 AC015396 31954-33705 *F MLTKLLKISCTSRQCTFAKPYQAIPGPRGPFGMGNLYNYLPGIGSYSWLRLHQAGQDKYE *F KYGAIVRETIVPGQDIVWLYDPKDIALLLNERDCPQRRSHLALAQYRKSRPDVYKTTGLL *F PTNGPEWWRIRAQVQKELSAPKSVRNFVRQVDGVTKEFIRFLQESRNGGAIDMLPKLTRL *F NLELTCLLTFGARLQSFTAQEQDPRSRSTRLMDAAETTNSCILPTDQGLQLWRFLETPSF *F RKLSQAQSYMESVALELVEENVRNGSVGSSLISAYVKNPELDRSDVVGTAADLLLAGIDT *F TSYASAFLLYHIARNPEVQQKLHEEARRVLPSAKDELSMDALRTDITYTRAVLKESLRLN *F PIAVGVGRILNQDAIFSGYFVPKGTTVVTQNMVACRLEQHFQDPLRFQPDRWLQHRSALN *F PYLVLPFGHGMRACIARRLAEQNMHILLLRLLREYELIWSGSDDEMGVKTLLINKPDAPV *F LIDLRLRRE *F >303a1 AC017306 12933-14444 no introns *F MFYTVIWIFCATLLAILFGGVRKPKRFPPGPAWYPIVGSALQVSQLRCRLGMFCKVIDVF *F ARQYVNPYGFYGLKIGKDKVVIAYTNDAISEMMTNEDIDGRPDGIFYRLRTFNSRLGVLL *F TDGEMWVEQRRFILRHLKNFGFARSGMMDIVHNEATCLLQDLKDKVLKSGGKQTRIEMHD *F LTSVYVLNTLWCMLSGRRYEPGSPEITQLLETFFELFKNIDMVGALFSHFPLLRFIAPNF *F SGYNGFVESHRSLYTFMSKEIELHRLTYKNYDEPRDLMDSYLRAQDEGNDEKGMFSDQSL *F LAICLDMFLAGSETTNKSLGFCFMHLVLQPEIQERAFQEIKEVVGLERIPEWSRDRTKLP *F YCEAITLEAVRMFMLHTFGIPHRAVCDTRLSGYEIPKDTMVIACFRGMLINPVDFPDPES *F FNPDRYLFDGHLKLPEAFNPFGFGRHRCMGDLLGRQNLFMFTTTVLQNFKMVAIPGQVPE *F EVPLEGATAAVKPYDIMLVAREQ *F >304a1 AC008359 comp(80887-82836) also AC014292 AC009393 *F MITETLLTICAAVFLCLSYRYAVGRPSGFPPGPPKIPLFGSYLFMLIINFKYLHKAALTL *F SRWYKSDIIGLHVGPFPVAVVHSADGVREILNNQVFDGRPQLFVAAMRDPGQDVRGIFFQ *F DGPLWKEQRRFILRYLRDFGFGRRFDQLELVIQEQLNDMLDLIRNGPKYPHEHEMVKSGG *F YRVLLPLLFNPFSANAHFYIVYNECLSREEMGKLVKLCQMGIQFQRNADDYGKMLSIIPW *F IRHIWPEWSGYNKLNESNLFVRQFFADFVDKYLDSYEEGVERNFMDVYIAEMRRGPGYGF *F NRDQLIMGLVDFSFPAFTAIGVQLSLLVQYLMLYPAVLRRVQNEIDEVVGCGRLPNLEDR *F KNLPFTEATIREGLRIETLVPSDVPHKALEDTELLGYRIPKDTIVVPSLYAFHSDARIWS *F DPEQFRPERFLDADGKLCLKLDVSLPFGAGKRLCAGETFARNMLFLVTATMCQHFDFVLG *F PNDRLPDLSQNLNGLIISPPDFWLQLQDRH *F >305a1 AC018013 comp(34574-37224) *F MSALIFLCAILIGFVIYSLISSARRPKNFPPGPRFVPWLGNTLQFRKEASAVGGQHILFE *F RWAKDFRSDLVGLKLGREYVVVALGHEMVKEVQLQEVFEGRPDNFFLRLRTMGTRKGITC *F TDGQLWYEHRHFAMKQMRNVGYGRSQMEHHIELEAEELLGQLERTEEQPIEPVTWLAQSV *F LNVLWCLIAGKRIARQEDGTLRRLLDLMNRRSKLFDICGGLLAQFPWLRHVAPDRTGYNL *F IQQLNTELYGFFMDTIEEHRRQLAKDPSPAESDLIYAYLQEMKDRSAGGESSTFNETQLV *F MTILDFFIAGSQTTSNTINLALMVLAMRPDVQEKLFSQVTASVAAASTDAFPHLSRREAF *F DYMDAFIMEVQRFFHITPITGPRRALWATKLGGYDIPKNATILISLRSVHLDKEHWKDPL *F EFRPERFIDSAGKCFKDEYFMPFGMGRRRCLGDALARACIFSFLVRIVQHFSVVLPAGES *F PSMVLLPGITLTPKPYKVQFVKRT *F >306a1 AC012373 154223-156412 also AC015216 AC012164 AC012376 *F MSADIVDIGHTGWMPSVQSLSILLVPGALVLVILYLCERQCNDLMGAPPPGPWGLPFLGY *F LPFLDARAPHKSLQKLAKRYGGIFELKMGRVPTVVLSDAALVRDFFRRDVMTGRAPLYLT *F HGIMGGFGIICAQEDIWRHARRETIDWLKALGMTRRPGELRARLERRIARGVDECVRLFD *F TEAKKSCASEVNPLPALHHSLGNIINDLVFGITYKRDDPDWLYLQRLQEEGVKLIGVSGV *F VNFLPWLRHLPANVRNIRFLLEGKAKTHAIYDRIVEACGQRLKEKQKVFKELQEQKRLQR *F QLEKEQLRQSKEADPSQEQSEADEDDEESDEEDTYEPECILEHFLAVRDTDSQLYCDDQL *F RHLLADLFGAGVDTSLATLRWFLLYLAREQRCQRRLHELLLPLGPSPTLEELEPLAYLRA *F CISETMRIRSVVPLGIPHGCKENFVVGDYFIKGGSMIVCSEWAIHMDPVAFPEPEEFRPE *F RFLTADGAYQAPPQFIPFSSGYRMCPGEEMARMILTLFTGRILRRFHLELPSGTEVDMAG *F ESGITLTPTPHMLRFTKLPAVEMRHAPDGAVVQD *F >307a1 AC014810 comp(54284-52654) also AC007840 revised 9/8/2000 *F MLAALIYTILAILLSVLATSYICIIYGVKRRVLQPVKTKNSTEINHNAYQKYTQAPGPRP *F WPIIGNLHLLDRYRDSPFAGFTALAQQYGDIYSLTFGHTRCLVVNNLELIREVLNQNGKV *F MSGRPDFIRYHKLFGGERSNSLALCDWSQLQQKRRNLARRHCSPREFSCFYMKMSQIGCE *F EMEHWNRELGNQLVPGEPINIKPLILKACANMFSQYMCSLRFDYDDVDFQQIVQYFDEIF *F WEINQGHPLDFLPWLYPFYQRHLNKIINWSSTIRGFIMERIIRHRELSVDLDEPDRDFTD *F ALLKSLLEDKDVSRNTIIFMLEDFIGGHSAVGNLVMLVLAYIAKNVDIGRRIQEEIDAII *F EEENRSINLLDMNAMPYTMATIFEVLRYSSSPIVPHVATEDTVISGYGVTKGTIVFINNY *F VLNTSEKFWVNPKEFNPLRFLEPDNEKLQLKRNIPHFLPFSIGKRTCIGQNLVRGFGFLV *F VVNVMQRYNISSHNPSTIKISPESLALPADCFPLVLTPREKIGPL *F Note there is a small intron between LEP ccgt agt DNEK *F No other animal P450 has an insertion here *F >307a2p AC019383 comp(3284-3706) AL078186 *F MLTSVFYVLFAIAITIILISYVFLLLKCKQKAFVVIGLLYQEKKYQCFDQAPGPHPWPII *F GNINLLGRFQYNPFYGFGTLTKKYGDIYSLSLGHTRCIVVNNVDLIKEVLNKNGKYFGGR *F PDFFRYHKLFGGDRNNCKFIXXLRF *F >308a1 AC015216 72624-74273 also AC012164 *F MLPLVLFILLAATLLFWKWQGNHWRRLGLEAPFGWPLVGNMLDFALGRRSYGEIYQEIYT *F RNPGLKYVGFYRLFNEPAILVRDQELLRQILVGRNFADCADNAVYVDHQRDVLASHNPFI *F ANGDRWRVLRADLVPLFTPSRVRQTLPHVARACQLLRDQVPLGRFEAKDLATRYTLQVVA *F SAIFGLDAHCLGIHMRVAHEPSRWLEWLAPLFQPSVWSLLETMSLLHTPRLGRLIGHRYV *F PLPLQHWFRELVEARSGGDNLLQWLAESKRGLGKEELAGHATTLLLEGYETSAMLLAFAL *F YELALNEDAQRRLHIELDEVAQRHAGNLIDPVALGELRYSEAALLEALRLHPAMQALQKR *F CTKTFTLPDQKSGASSELKVHLGTVLVLPVQAIHLDPALYPAPNQFRPERFLNQPPMGCR *F FLGFGAGPRMCPGMRLGLLQTKAALTTLLQDHCVQLADEDQCRVEVSPLTFLTASRNGIW *F LSFKRRTRRY *F >309a1 AC019891 comp(47437-49308) also AC009909 *F MFTLVGLCLTIVHVAFAVVYFYLTWYHKYWDKRGVVTAEPLTILGSYPGILINKSRSLIL *F DVQDVYNKYKDKYRTVGTFITRQPQLLVLDPALAHEILVDKFSHFRDTITSSFVGHNPDD *F KYVAGSPFFSAGDKWKRLRSENVGGLTPSRLKMAYSIWEQSGRKLVEYIERARREQGDII *F ETRDLAYRFTANAMADFIWGIDAGSLSGKVGEIGDFQKTSTDWSAHAFSSMIRFNKTLVA *F IFVRKLFSMRFFTKATDEFFLRLTQDAVNLRQGGSGEGRTDYLSHLIQLQQRGNSIHDSV *F GHALTVHLDGFETSGAVLYHMLYSLSEHHEEQEKLRSEILEALASEGQISYDQINNLPYL *F DQCFNESLRLTTPIGFFMRICTKPTQINLGDDKTLDLEPGVTVMVPAYQYHHDNDIYPEA *F SEFRPDRFENGAASVLTKRGCFLPFGDGPRICLGMRVGQLSVKTAIVHILSNYQVEQMKK *F VPLGADSGMGIFLNGDVELKYTKLQK *F >309a2 AC009909 45541-45982 also AC019977 *F MYILASLALILLHLLVLPIYLYLTWHHKYWRKRGLVTARPLTLLGTYPGLLTRKSNLVFD *F VQKIYDKYKGKHRAVGVFVTRQPQILVLDPELAHEVLVSNFRCYKDSLQSSYLRHAKWDK *F YARLNPFWASGQSWRRLRTDAQAGISGSRLRQAYNIWEQGGQMLTEYMTQQVAEKNNILE *F TRDLCFRYTAHVMADFIWGIDAGTLTRPMEQPNKVQEMASKWTSYAFYMLTLFMATIVAP *F CSRLLLRFRFYPKETDEFFSNLTKESIELRLKAGDSTRTDYLSHLLQLRDQKQATHDDLV *F GHALTVMLDGYDTSGTALLHALYYLAENPAVQQKLRVEILSCMASEKSLDFEKLSSLQYL *F EQCFNESLRLSSLIPQYTKVCTLPTVIRLSESKSLDVEVGMTIMIPNYQFHHDKQYFPEP *F EAFKPERFDNGAYQELMRKGIFLPFSDGPRICMGVPLAMLTLKSALVHILSNFQVVRGRD *F RLIPKGDSGFGVVLQGDVNLEYRRFFR *F >310a1 AC017690 comp(12877-14650) *F MWLLLPILLYSAVFLSVRHIYSHWRRRGFPSEKAGITWSFLQKAYRREFRHVEAICEAYQ *F SGKDRLLGIYCFFRPVLLVRNVELAQTILQQSNGHFSELKWDYISGYRRFNLLEKLAPMF *F GTKRLSEMFGQVQKVGDHLIHHLLDRQGQGCPQEVDIQQKLRVYSVNIIANLIYGLDINN *F FEHEDHILTSYLSHSQASIQSFTLGRLPQKSSYTYRLRDLIKQSVELREDHGLIRKDILQ *F LLVRFRNGNEVSGDKWQLEPINDADKLLSIKRLAKVAEDLLKVSLDAVASTVTFTLLEIL *F QEPLIVEKLRAEIKELSNENGQLKFEELNGLRYMDMCLKETLRKYPPLPIIERVCRKSYS *F LPNSKFTIDEGKTLMVPLLAMHRDEKYFSEPMKYKPLRFLQTANDVGQCEDKTKSNVFIG *F FGIGGSQCVGQNFAKLVIKVALIKLLQNFHLELDANQVKTLKVSHRPAPFIHTKDGLKVK *F LKRREINTKFYS *F >311a1 AC014186 2270-3910 *F MALWPLLLITLTIWILVRKWTLLRLGSSLPGPWAFPLLGNAQMVGKLRPEYIFLVFTELR *F DRFGATYRLRLGPQLWVFLHSAEETRQALHDPTLRKADTFMQLEPLIGNGLLISHGAHWT *F RQRRLLTPAFQPQLLRSFAPAIGGHVERLVGRLGATRGAFLEVTEPLFACLLDAIVDTSM *F GAQLDTQSVDHSPIIQAFHLSSKLLFKRMINPLLSSDWIFQRTQLWRDLDEQLQVIHSQM *F ESVIEKRAKELLDMGEPAGRAHNLLDTLLLAKFEGQSLSRREIRDEINTFVFAGVDTTTA *F AMSFVLYALAKFPETQTRLRKELQDVALDETTDLDALNGLPYLEALIKEVLRLYTIVPTT *F GRQTTQSTEIGGRTYCAGVTLWINMYGLAHDKEYYPDPYAFKPERWLPEDGAVAPPAFSY *F IPFSGGPHVCIGRRYSLLLMKLLTARLVREFQMELSPEQAPLRLEAQMVLKAQQGINVSF *F LKQ *F >312a1 AC015424 57845-59744 also AC009369 AC007573 AC010003 *F MFWLGFGLLLLALSLYLLYVFERQSRIDRLTHKWPAPPALPFIGHLHILAKLVGPHPLRR *F ATEMINEHLHDHRAKLWMGTKLYLVDCNPKDIQALCSAQQLLQKTNDYRVFENWLCEGLF *F TSGFEKWSHRRKIVMPAFNYTMIKQFVAVFEKQSRILLTNVAKFAESGDQIDFLQLISCF *F TLDTICETALGVSVGSQSSAKSEYLDAVKSILVIIDKRLKNIFYRNSFIFKRTSHYKREQ *F ELIKTLHGFTEGIIQKRIDEINQDAENRNYQSSDAELDGVKRTLCFLDTLLLSKGPDGKP *F LTVKDIREEVDTIIFGGFDLTATTLNFFMYNMTLHPEHQQRCREEVWSVCGKDKSEPISI *F EQVRQLEFLEACIKETLRMYPSGPLTARKATANCTINDFFIPKGSDVIISPIYMGRCKDF *F FPDPMVFKPDRWAIGAEPKIEATTFIPFMAGARSCMGQRYAMVMLKMVLAHLLRNFLFEP *F LGERQVKLKLNFVITLHTVEPYLCRAKNLD *F >313a1 AC017740 comp(167857-169728) also AC007809 AC007928 *F revised 9/11/2000 *F MLTINLLLAVGALFWIYFLWSRRRLYFLMLKIPGPIGLPILGSSLENIITYKRKLSFRTK *F YLNKYGSTILTWMGPVPFIVTRDPKVVEDIFSSPDCHNKSQHIVNAITSCMGNGLLGKQD *F PHWLDRRKHFNPSFKQDLLLSFFHIFDAETKVLMNLLDTYVDKGEIDVVPEMLRWSFKIA *F AQTTMGSEVKHDEHFKNGSLVESFESLISHSTLNILMPLVQNRMISKICGYDKLRADNFS *F RIQKMLDNVVNKKVNPLPKTDSDPESNIVINRAMELYRKGDITYMDVKSECCIMIAAGYD *F TSALTVYHALFLLANHPEHQEAVFEELNGVFPDAGHFGITYPDMQKLDYLERVIKETLRL *F IPAIPITARETKNDVRLSNGVLIPKGVVIGIDMFHTHRNPEVWGPDADNFNPDNFLAENM *F EQKHPYAYIPFARGKRNCIGSKYAMMSSKFALCRILRNYKISTSTLYKDLVYVDNMTMKL *F AEYPRLKLQRRG *F >313a2 AC017371 4100-5929 AC007724 AL057969 AL067059 rev. 9/8/2000 *F MIVIQLLIAASLILWIRFLWSRRKLYMLMMQLPGRMGLPLLGNSVRYLIISRGKMSSRTT *F YMDKHGSTYMAWIGTTPIVITRDPKIAEKVLTSPFCINRSSQTTNALALSMGYGLLTLQ *F GSKWMARRKHMNPAFKHSVLLSFLPIFNAETDLLVSVFDSFVGQGEKDVLSDLIRWSFAI *F ATQTTLGTDVTKDDNFENDAILKTYQSMLRLTIINIFVPFVQNKIVSKLFGLEWLRRRDA *F SAINKMINNILDKKLNSNPENYCESELKTVIHRAIELFRNDEMSLMELGAECSSMVLAAF *F ETSAHTVYYALVLLAMFPEHQEMVFNEIKEHFPLAKGIEVTHTDLQQLVYLDRVLNETLR *F LMPSVPFSSRETLEDLRLSNGVVIPKGMTISIDIFNTQRNTDYWGSEAAQFNPENFLPEK *F IHDRHPYAFIPFSKGKRNCIGWRYGLMSSKLALVKILRNYKLKTSFPYENLEFVDHMVIK *F LAQSPQLAFERRTL* *F >313a3 AC017371 1890-3720 also AC007724 *F MDTFQLLLAVGVCFWIYFLWSRRRLYMMHFKIPGPMGLPILGIAFEYLITYKRKMSIRTK *F YMDIYGSTCLVWVGPTPFVITRDPKIAEEIFLSPECLNRSSIFSKPVNSCTGDGLLSLEA *F SKWVDRRKNLNPAFKQNVLLSFLPIFNSEAKTLVAFLDSLVGQGEKKVRDDIVRWSFRIA *F TQTTVGTDVKKDASFKNDSVLKSYETFMKIIVMNVLLPFTHNKIFSTLGGFETQKALAKS *F NVNKMIGTIVDKKLMTKPESGSQPEITSVINKAIELHRNGEMSREEVQSECCSFVVAAFE *F TTGDTVYHALILLAMFPEHQDTVYQELKELFPVAGDFEVTYDDLQRMVFLERVVNETLRL *F IPSVPFTPRETIRDFRLSSGVVIPKGVGIGIDIFATHRNRDHWGTDPSSFNPDHFLPDNV *F RDRHPYAYIPFSKGRRNCIGWKYGLMSSKLALSKILRNCKVSTSFRYEDLEFVDNIGMEL *F AQSPGLEFHRRT* *F >313a4 AC017336 153877-155807 also AC007752 *F MLTWTLWCGLLFLLWIYFLWSRRRFYLLTLKIPGPLGYPILGMAHWLMRREDILNAFGCF *F LDKHGPTIFSWLGPIPFMIVSDPQVVQDIFTSPHCVNKGIIYKAVDDGAGVGLFSLKDPR *F WSIHRKLLNPAFGHKVLLSFLPIFNRETALLLDQLEPLQDDGEKDLIPLLQSFTLGIATQ *F TTMGSDVKDEESFRSNSLLGRYQCILETMTDMCFSPWLNSRFCRQLAGKESHYYQAKTEI *F RQFIRKIIERKLAEDEMGALPSIQSNDKNLFLNLVTDLMRRGVFTLKNVEDESNIIVFGA *F FETTANAVYYTLMLLAMFPEYQERAFEEIKTIFPNTGDFDVSYADTQQMVYLDLILNESM *F RVIPPVPVVSRQTSQDLKLSNGIVVPKGVQIAIDIYHMHRSKKIWGPDAETFNPDHFLPH *F NIQDKHPYAYIPFTKGIRNCIGWRYALISAKVTLAKLLRNYRFKTSFPFENLYFVEDITM *F KLKSVPLLELQKRT *F >313a5 AC017371 6352-8145 also AC007724 rev. 9/8/2000 *F MLTLQIFEAFAIILCVYFLWSRRRFYIMMLKLPGPMGFPFIGLAFEYIRLKRKIRLRT *F ILFKIYGKTVLTWIGLTPVLVTCEPKILEDIFTSPNCSNRSSVVDKAISSCLGLGLLTLK *F NNHWNERRKLLLPSFKNNAVLSFVPVLNNEANFLVTLLAEFVDGGDINLLPELNKWSFKI *F AAQITMGDEVRNQANYQNGNLLESYKALNNLIPIGVVMPWLRNKYLGKLFSYEKRRLEAA *F TQSNAFIKDIIDKKLSSTDNSSEPALIDRILNLVRIGELSYDDVMGEFSNIIFAASDTLS *F ITVNNVLILMAMFPKYQDNVFEELAEVFPSGGEFEASHADLEKLVKLDRVLHETMRLIPA *F VPLLIRQTSHSIQLSNGFYIPEGVTLMIDIFHTHRNKDIWGPQANAFNPDNFLPENKRAR *F PPYSYLPFSKGKKTCLGWKLSLISAKLALAKILRNYMLSTTFLYKDLRFIDNTTMKLAEQ *F PLLAVKRRI* *F >313b1 AC007571 comp(104264-106046) also AC013225 revised 9/11/2000 *F MLASIILSGWLLLAWLYFLWSRRRYYKVAWQLRGPIGWPLIGMGLQMMNPESKSWTGFFK *F APFISWMGTSCFLYINDPHSVEQILNSTHCTNKGDFYRFMSSAIGDGLFTSSSPRWHKHR *F RLINPAFGRQILSNFLPIFNAEAEVLLQKLELEGVQHGKRLEIYQILKKIVLEAACQTTM *F GKKMNFQHDGSLCIFKAYNGLTEVCVKRMLSPWLYPDLIYRRSGLFRLQQKVVGILFGFI *F EQLLEPIVSVVAANSNPDQQRSEMEMRGKSKAIFIEQVREHVERGQLSWQDVRDEANVTI *F AATFETTSTALYFTILCLAMHPCYQEKLHKELVTELPPSGDINLEQLQRLEYTEMVINEA *F MRLFAPVPMVLRSADQDIQLKRGDGEFLIPRGTQIGIDIYNMQRDERVWGPLSRTYNPDA *F HFGLDSPQRHAFAFVPFTKGLRMCIGYRYAQMLMKLLLARIFRSYRISTEARLEELLVKG *F NISLKLKDYPLCRVERR *F >314a1 AC012699 22585-24723 also AC006496 *F MAVILLLALALVLGCYCALHRHKLADIYLRPLLKNTLLEDFYHAELIQPEAPKRRRRGIW *F DIPGPKRIPFLGTKWIFLLFFRRYKMTKLHEVYADLNRQYGDIVLEVMPSNVPIVHLYNR *F DDLEKVLKYPSKYPFRPPTEIIVMYRQSRPDRYASVGIVNEQGPMWQRLRSSLTSSITSP *F RVLQNFLPALNAVCDDFIELLRARRDPDTLVVPNFEELANLMGLEAVCTLMLGRRMGFLA *F IDTKQPQKISQLAAAVKQLFISQRDSYYGLGLWKYFPTKTYRDFARAEDLIYDVISEIID *F HELEELKKSAACEDDEAAGLRSIFLNILELKDLDIRDKKSAIIDFIAAGIETLANTLLFV *F LSSVTGDPGAMPRILSEFCEYRDTNILQDALTNATYTKACIQESYRLRPTAFCLARILEE *F DMELSGYSLNAGTVVLCQNMIACHKDSNFQGAKQFTPERWIDPATENFTVNVDNASIVVP *F FGVGRRSCPGKRFVEMEVVLLLAKMVLAFDVSFVKPLETEFEFLLAPKTPLSLRLSDRVF *F >315a1 AC015141 229-2538 also AC008307 AC007725 AC007648 *F MTEKRERPGPLRWLRHLLDQLLVRILSLSLFRSRCDPPPLQRFPATELPPAVAAKYVPIP *F RVKGLPVVGTLVDLIAAGGATHLHKYIDARHKQYGPIFRERLGGTQDAVFVSSANLMRGV *F FQHEGQYPQHPLPDAWTLYNQQHACQRGLFFMEGAEWLHNRRILNRLLLNGNLNWMDVHI *F ESCTRRMVDQWKRRTAEAAAIPLAESGEIRSYELPLLEQQLYRWSIEVLCCIMFGTSVLT *F CPKIQSSLDYFTQIVHKVFEHSSRLMTFPPRLAQILRLPIWRDFEANVDEVLREGAAIID *F HCIRVQEDQRRPHDEALYHRLQAADVPGDMIKRIFVDLVIAAGDTTAFSSQWALFALSKE *F PRLQQRLAKERATNDSRLMHGLIKESLRLYPVAPFIGRYLPQDAQLGGHFIEKDTMVLLS *F LYTAGRDPSHFEQPERVLPERWCIGETEQVHKSHGSLPFAIGQRSCIGRRVALKQLHSLL *F GRCAAQFEMSCLNEMPVDSVLRMVTVPDRTLRLALRPRTE *F >316a1 AC017388 35538-37158 also AC010113 AC010557 AC010112 *F MILTATFICFCLASAFNYFRARRQRSLIKNLKGPFTWPLMGAMHKLLFLTPINFFQRSTE *F YLTKYGTFSRCWVFHRLFIPLADLELSRQLLENDTHLETGYELMKDWLVGGVLMCQSEQW *F QKRHSLISGLFDKGNLEQLIDLSRHQTEQLLQKLAKQADQKVFDIWYTVSPIVLDLMVMT *F TCGAKPSEEYSKNLKDLSEIYRKRFLSLQSANRFNYWLSSPFMRKRQNRLIKRLNDEHNN *F LMAMHQSQNQLKIENGLDIYQLRPIPLKDHKSLLEILLESKDPQLTGEEICGELNTCNYL *F GYQLCSPALCFCLVTIARNPSVQQKCLDELNLAQIKDQGWDLEKLNYLDAVLHETMRLYP *F PQVIVGRQLKKDFPYNAELPCGSEIYINLYELQRNEVRYPKANHFDAQRFLDSPPELLSY *F SLGPRCCPARKFSMQLLKTLLAPILANFEVLPYGDEVRLDLRLVLGSSNGFQLALKPR *F >317a1 AC020477 126550-128106 no introns also AC009844 AL109349 *F MWIIFLIIGLLVLGLLVLLIIAARYQRDYWRYLDIPHERPKKLWPIIRQIMTQTLSTEAM *F KAEHYSAIYKKFKGSGPFCGFYALLQPRALILDRELIRQIMIKDFWNFNDRGLYCNQKSD *F PLSGDLYALRGESWKEMRQKLDPSLEGDRMSLLYDCLYEEAEQLLLTVNSTLMSQPHSTV *F HIQKIMRRYVLSSLAKCVFGLNAEQRKTYPLEDFEQMTELALNSHKHGYLMNLMMIRFPN *F FCRMLRMRRTPKQAEEYFIKLLTSIVEQRETSGKPQKDYLQLLIDVKALEFITYQYEADK *F ELGAHLQNELAAHADVFLKAGYEQTANTLSYVLYELALHPELQVRVREEVKKAIERHDGH *F ITHEGIKSLSFMGQVINETLRMHPITPYILRRTLNDYAVPDHPKYILVKELFLIIPTHAI *F HHDPDIYPDPEEFKPDRWSGPRDSLQEQGTWFGFGVGARSCIGIQFAQLQLRLALALLLS *F EYEFSLNTRKPLINLEDGIALTLMPLGVIEPGNEERAV *F >318a1 AC014186 comp(968-207) AA803931 AA803220 AA821188 rev 9/11/2000 *F MCCLQSTTLDRKKNASWNRSAIVGSDTSDAPEHCPLRCGALLAVLLAWQQRKCWRLIWQL *F NGWRGVIQQPVLWLLLCINLHPNSILEKVSQYRVHFQRPLRVLVGTRVLLYIDDPAGMEC *F VLNAPECLDKTFLQDGFFVRRGLLHARGQKWKLRRKQLNPAFSHNIVASFFDVFNSVGNQ *F MVEQFQTQTNLHGQAVKFTAAEDLLSRAVLE *F (small gap) AC019897 comp(2937-4007) seq. is incomplete in *F Celera AE003488 missing the ETAM exon (about 30 amino acids) *F EESKKCAKLLEDFVGGIVRTKHRNWRLRDAVGGEKSGEDASNGWQRRIFIEQIFQLAANG *F EMTLEEIMDEAQSMVLVVGLKISYLNTIYFIFYCNFQSFETVSNSIMLALLCLATNKGDC *F QRRLLAEIRALVPDVGQVGLEQLQQLRYLDAFVSESLRLLATVPMNLRHVSRDFRLAGRQ *F HETIVPQNSIVVLDTFNMQRDERWWGANARQFDPQRFLDQEEEQLSKGHNDSGSGEKRRQ *F RDRRHSYSFLPFSNGLRSCIGRRYGLFIMKVFLVKLITNFDFQSDFELEKLQFVENISLK *F FKNADDILLTIQPKKEST # *U FBrf0136022 *a Whitfield *b E. *t 2001.2.5 *T personal communication to FlyBase *u FlyBase error report for Acp98AB on Mon Feb 05 11:30:28 2001. *F From eleanor@ebi.ac.uk Mon Feb 05 11:28:27 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 5 Feb 2001 11:28:27 \+0000 *F Date: Mon, 05 Feb 2001 11:30:28 \+0000 *F From: Eleanor Whitfield *F X-Mailer: Mozilla 4.75 en (Win95; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F CC: Eleanor Whitfield *F Subject: Celera translation for Acp98AB *F Content-Transfer-Encoding: 7bit *F Hi, *F Currently Celera has no annotation for Acp98AB. *F Using BLAST of DNA from Wolfner et al, Insect Biochem. Mol. Biol. *F 27:825-834(1997) (U85762 and U90949) and Begun et al, Genetics *F 156:1879-1888(2000) (AAG38159-AAG38168) I believe the translation is on *F AE003762. *F I have only found aa 5-31 of the 31 aa protein. I am unsure what has *F happened to the first 4 aa. Maybe you can trace them? *F thanks *F Nellie # *U FBrf0136023 *a Robertson *b H. *t 2001.5.6 *T personal communication to FlyBase *u FlyBase error report on Sun May 06 00:40:30 2001. *F From hughrobe@life.uiuc.edu Sun May 06 06:40:24 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sun, 6 May 2001 06:40:24 \+0100 *F Date: Sun, 06 May 2001 00:40:30 \-0500 *F From: hughrobe@life.uiuc.edu *F Subject: more bee-suggested annotation improvements *F To: Millburn_Gillian *F X-Authenticated: *F MIME-Version: 1.0 *F Content-Type: MULTIPART/MIXED; BOUNDARY='67288700-0--1096868866=:9000' *F Content-Length: 76024 *F Dear Gillian, *F Early this year I sent to you, via Sima Misra, a set of unannotated genes, *F plus some annotation improvements, suggested by some of the 15,000 honey bee *F brain ESTs we have. I've been working on this more, and have about another 40 *F such instances, only 2-3 of which are completely unannotated genes in *F Drosophila. There are many more instances like these and I will try to get *F them to you soon. For now the attached WORD MAC TEXT file has a simple format *F of each bee EST sequence with a short note about what I think it suggests, but *F I leave working up the details of the needed changes to the Drosophila gene *F annotations to you folk. *F Hugh *F Hugh M. Robertson *F Professor *F Department of Entomology *F University of Illinois at Urbana-Champaign *F 320 Morrill Hall, MC118 *F 505 S. Goodwin *F Urbana, IL 61801 *F Phone 217-333-0489 *F FAX 217-244-3499 *F Email hughrobe@life.uiuc.edu *F www.life.uiuc.edu/robertson/lab.html *F \------------------------------------------------------------------------------ *F -- *F More honey bee interestings things, found by actually comparing TBLASTX and *F BLASTX scores; *F wherever the former is much higher than the latter one might expect that the *F BLASTX found a member of a gene family, while the TBLASTX found the real *F ortholog *F Also, now comparing BLASTX to nr with all of these searches of the Drosophila *F genome, and those coparisons turn up additional annotation improvements. *F I've not worked any of these up in detail, simply note the honey bee EST that *F provides the data, and some indication of the problem. *F \***************A. mellifera BB260004B20D3.F *F AGATCGCCCTCACGACAACATCCCCGTGGCTCCTCTTCTTCGACCTGGTCGCCATTTACAAAGACGACCCGGACCTGAG *F GCTCTGCCTCGAGGTGTGGCCGCGCCCGAAAGACGAAACCCTCTTCTTCCTGATCGGCAATCTGACCCTGTGCTACGTA *F CTACCCACGATCCTCATCTCCCTCTGCTACATATTGATCTGGATCAAGGTGTGGCGGAGGCACATACCCTCCGACACGA *F AGGACGCCCAAATGGAGAGGATACAGCAGAAGTCGAAGGTGAAGGTGGTGAAAATGTTGGTCGTGGTCGTAATACTGTT *F CGTCCTCTCGTGGCTACCCCTCTACGTCATCTTCACTGTGATCAAGCTGGGCGACGAGCAAAGGGAGGACGAGATCGTC *F CCCATAGCAACGCCGATCGCCCAATGGCTGGGAGCGAGCAACTCGTGCATCAATCCGATCCTCTACGCCTTCTTCAACA *F AAAAGTATCGGCGAGGCTTCGTCGCGATTCTGAAG *F Very unusual example. The EST has two forward ORFs. One matches the *F translation of CG10823 at about e-18, but there is a TBLASTX genomic match at *F e-60 for the other *F Turns out that the latter is the correct translation, matching as it does *F tachykinin receptors in vertebrates, and the Drosophila annotation of CG10823 *F is using the wrong reading frame! *F \***************A. mellifera BB260006A20B4.F *F TGACTGAAGAAACACAACAGTCTGAGACTGCCGCACAAAATGAGGCACAAACTAGTTCTCCAGATGTTGGAAAAGTTAA *F AGATAGTAAAAAAGAAAAATGCCGACCTATGACAAAGGTAGTAATACGGAGATTACCTCCAACTATGACTCAAGAACAA *F TTTCTAGAACAGGTTTCTCCATTGCCAGAAAATGATTATCTTTATTTTGTGAAAGCTGATATGTCTATGGGACAATATG *F CTTTTGCCCGTGCTTATATTAACTTTGTTGAACAACAGGATATTTTTATGTTCAGAGAAAAATTTGATAATTATGTATT *F TATCGACTCTAAAGGTACAGAATATCCAGCTGTAGTAGAATTTGCACCTTTTCAAAGATTACCAAAAAAAAGAACAGGA *F AAAAAGAAAGATTTAAAATGTGGTACAATAGAATCAGATCCTTATTATATAAGCTTCTTAGAAACTCGTAAAAATCAAG *F AAGCTGAATCTAATATATCACAACCAAAAACAGAATACTCATATCAACCACCTGATAATACACCAAAAAAAATTACAAC *F CACTCCTCTTTTGGAATATGTAAAACAACGTAAACAAGAAAAGCAACGTCTCAGAGATGAAAAACGTGAAGAGAGACGA *F CGAAGAGACCTAGAACGGAGGCGAACAAAAGAAGATCCTATCATATCTAAGGTATTGAAAATCAAGATCTTGATAAAGA *F AATGTGTAAAGATTATAAAGAAATAGGGAAGAAAAGATAAT *F Shows that the N-terminus of CG11184 is missing *F \***************A. mellifera BB260007B10E5.F *F GCTACAAGAAAAAGTATTAGCCGAAACATCAATAAAAAAAGAAAATAAAGACAACACTGCAAGTGGTGGCGAATCTTTA *F GAAACGGTTTTAGAAACAGAAACAACTGAAAATGTAGAGGAAACCTTTAATGATCAAGAAAGTATACCTTATTGCGTGA *F AGACTAAACCTTATTATTCTATTAATGCGAAATTAAATACAAGAGAAAAAATTAATATTCCTTCCATTAATGCTTCCAC *F CAACAAATATGTTGAGAAAACATATTCGGAAAGTGGAATAGATGAGAGTACACCTGCTTTAGAAGATCAAATTACTCCA *F ATAGAAGATACAAAACCTCCTCCTGTTTGGGATGCGAATTTTAAATTTCTTACTAGCTCTGTGAGCGGTAAAAGTTCTC *F AGAATTATAATAAATCTAGTGTTACTTGTCTTATTTGCGGAAAGCAATTAAGTAATCAATATAATTTGCGAGTACATAT *F GGAAACTCATAGTAATAGTTCATATAACTGCACAGCTTGTTCACATGTATCAAGATCACGAGATGCCCTTAGAAAGCAC *F GTTTCTTATAGACATCCAATGGCCTCGCCACAAAAACGTTCACGTTATAGTACACCGAAATCCTAAAAAATGGTATCTT *F TAATCTTCGATTAAATCTTTGATCTAAGAATAGGACGTTTTAATTAAAACATAAAACTTTGTGTAATTTAATGCCCAAA *F TTTTTAACGGTTAATGGTCTAAGGAAATGCCGATTGATTTAAAAAAAAATAAAAGCCA *F Encodes C-terminus of a > 200aa protein *F LQEKVLAETSIKKENKDNTASGGESLETVLETETTENVEETFNDQESIPYCVKTKPYYSINAKLNTREKINIPSINAST *F NKYVEKTYSESGIDESTPALEDQITPIEDTKPPPVWDANFKFLTSSVSGKSSQNYNKSSVTCLICGKQLSNQYNLRVHM *F ETHSNSSYNCTACSHVSRSRDALRKHVSYRHPMASPQKRSRYSTPKS *F BLASTX match is weak at e-06 to multiple four-cysteine repeats within CG2889; *F but genomic e-15 match is to a single four-cysteine repeat interupted by an *F intron; N-terminus doesn't align *F Genomic match is to 7kb unannotated region \- try to reconstruct gene below: *F no ESTs to help *F I think there are two more exons upstream, with excellent splice sites, but *F they don't splice in frame to this below, so unsure what's going on in absence *F of cDNA *F ATGCCAGACAACCGCAGCATCATCATTTTGATCAGCAGCTGCAGCACAATGACAGCCAACAGCAATTCTGCCTGCGATG *F GCATAACCATCAGgtgagatcctccagataaaattcatttgaattttcatttgtggggggactatattcaacattacct *F cataagtaagatccattagaatggactggacatcgttcagtgaagcgagcaggccgaaagtaaaatgtcgccgcatcta *F tatataggatccatctatccaaacacattcccctatacgtacagattcacacatagttttccgcattttcatatagtga *F caatttttgtgtttcggcccaaagggtgtaatgcggtgaaagcggggcagcggtgaaactggctgtccaaaatagcaaa *F acagccaaatggacaaagagtagtgaggaggagtgttcagtgtgtgtgtcacataaatgaacagattcgtgcaattgcc *F aaaatccaaagaatttccacagcgagtaaatcgaaaagttggaaagcagctgcagccaacccctgtcccctgttacacc *F gcagacagaggagcatcatggggcccaggaaattgtatttttatgatcgtgtgacccacttatgagcacttttcacatc *F cccaaccccattccctctgatcccttccagACAAGTTTGCTGAGCACTCTGCCCATTCTGCTGGACCAATCCCATCTGA *F CGGATGTGACCATTTCGGCCGAGGGACGCCAACTGAGGGCCCATCGCGTGGTACTGAGTGCCTGCAGCAGCTTTTTTAT *F GGACATCTTCCGGGCTCTAGAGGCCAGTAACCATCCAGTCATCATCATACCAGGGGCCAGCTTCGGCGCCATCGTCTCA *F CTGCTCACCTTCATGTACTCCGGAGAGGTGAATGTATACGAGGAGCAGATACCGATGCTGCTCAACCTGGCCGAGACAC *F TGGGCATCAAGGGACTGGCCGATGTCCAGAACAACAATgtgagtagctcaagtgcaagatctagttagataatttaaat *F aaacttgtagTTTTTCCCTGAAACTCATACGCCTTTCTCTTTGGTCTCATAAATCCAAGCAGTTACCAAAAACAGCGAG *F AAGTGGAGGTGGCTCCTAC *F And not even sure about the C-terminus, although it does align somewhat *F AE003538.2 *F ATGGATACGACGAATGAGAAGTCCTCTGAGTTTGAACGTCCCACCACACCCTCGCCCACGCCCACCCCCACCCTTACGC *F CCTCCCACACGCCTACCCCAAGCCATTCACTCCCCCTGCCCCAGCTACCAAGTGCAGCACTTAACACCCCTCTCCTGGC *F CAACAAACTGGGATCGGTGAACTCCAGTGGAATGGGCACCACGCCCCTGGAGAATCTCTTTAAATCACTGCAGTTCTAC *F CCCAGTCTGCTGCCCCAACCACTCAACTTCTCGCAGACGGCGCTCAACAAGACCACTGAGCTGTTGGCCAAGTATCAGC *F AGCAATGTCAGCTCTACCAGAGCGGGATGCAGGAGGATCAACTGGAGACGGACTGTTTTGGCTCCAAGAGGCTAAAGGG *F CGACAGTCCGCCGAAGGAGCTTAGGCGACTGGAAAAAAGCCTTTTAAAGAATCCAAAATCCTCATCGACGACCAACAGT *F AGCAGTAGCAAGTCGCCTCAGGAATGCTCCAACCCGAATCCCATTGTGGCCACTTCACCAGTAACTCTCGCTCCCCCGA *F CCATGGCACACTTCTCCCCTCAGTTGCCGGTGGTCAAGTGCTCCTCGGCTAGTTACCCCAGCGCTCTCGGCCAAGGTCA *F ACTCTATAGTAGCAAGCCACCACTTTATAGTGCAGCAGTCACGCCGACTGCCGCCCAGCAGGCGGCGCAGATGCACCAC *F CACCCGCAACCCGGGCCATCGCCCTACATCTCGGCCGAGGATCATGCCAAGCTGCAGCTCCACATCGAACAGTATCAGC *F GGGAGGCGGCAGCAGCAGCGGCAGCGGCCGCCGGCGGAATGGCGCTGGTCAGCGCCAAGTCGGAGCCCAATCTGCTCTC *F GCTGAGCGCCGACCGCGACAAGTCGCTGGCCACCGCTCCCATCAAGCCGCCGTCCAACTCGAAGCTCTATGCCACCTGT *F TTCATCTGCCACAAGCAGCTGAGCAACCAATACAACCTGCGCGTCCACCTCGAAACCCATCAGAATGTTCGgtaagtgg *F tctgaattttaattgttataaaacaatagaagtcacctttggaattacttttcgattccatcagGTATGCCTGCAATGT *F CTGCTCCCATGTGTCCCGCAGCAAGGATGCCCTGCGCAAGCACGTTAGCTACCGACATCCTGGGGCGCCATCGCCATGT *F CGAAAACGAGGCTCGCCGGAAGAGGGTCTCCAAGCTAGCAGCGACCACTGTGCCCACTTCCACGCCCATGTCCATGAGC *F GCCAGTCACACGGTCACCAGTGGCGATGTGGGTCCAGCTCCAGCGACCACACTTGGATGTTCGGGTCAGGAGGCAAGGA *F ATCCGTACCTTTTCCTGCCCAATCAATTTCAGATGGCTGCTGCTGCAGCAGCCGTAGCAGTGGCCGAATCCTCGCCAGC *F TTCTGGTCAACCATCGCTAGACTTGGCACACGAAGCGCCACCGAGCATCAAAAGTGAGCGGGAGCCACCGACGGCGAGC *F AACGGAGAGGCGACCGGTGTAGAGGCATCGGCGTCAACCACCTGAGGATAATTTTTTTGAATATTTTT *F translation M D T T N E K S S E F E R P T T P S P *F T P T P T L T P S H T P T P S H S L P L P Q L P S A *F A L N T P L L A N K L G S V N S S G M G T T P L E N *F L F K S L Q F Y P S L L P Q P L N F S Q T A L N K T *F T E L L A K Y Q Q Q C Q L Y Q S G M Q E D Q L E T D *F C F G S K R L K G D S P P K E L R R L E K S L L K N *F P K S S S T T N S S S S K S P Q E C S N P N P I V A *F T S P V T L A P P T M A H F S P Q L P V V K C S S A *F S Y P S A L G Q G Q L Y S S K P P L Y S A A V T P T *F A A Q Q A A Q M H H H P Q P G P S P Y I S A E D H A *F K L Q L H I E Q Y Q R E A A A A A A A A A G G M A L *F V S A K S E P N L L S L S A D R D K S L A T A P I K *F P P S N S K L Y A T C F I C H K Q L S N Q Y N L R V *F H L E T H Q N V *F R---------------------------------------2-------------------------------- Y *F A C N V C S H V S R S K D A L R K H V S Y R H P G A *F P S P C R K R G S P E E G L Q A S S D H C A H F H A *F H V H E R Q S H G H Q W R C G S S S S D H T W M F G *F S G G K E S V P F P A Q S I S D G C C C S S R S S G *F R I L A S F W S T I A R L G T R S A T E H Q K Z *F Leave it at this for now. encodes weak similarity to several other zinc finger *F proteins in Drosophila, e.g. sob *F \*********** extra \- there is a single testis EST for near the end of this *F 7kb section, beyond this gene, which could encode a small protein; no matches *F at all. *F bs85b10 5' *F GCACCAGGTTTTTCGTCTTCTGCACTCGAGCGCCTTTCTAGCTATCTTAATTTATGTACTTACGTTTAGCACAATTTAC *F GTTTATTTTTTCTGAAACTTTAGCAACCTCCGAGGCTCTCAAACTGCAAGTTACAAACACAATCCTTTCGCTTTCACGC *F TCTCTCACAAGCACACGTCCACACATTCTAGTAATCTAAGCCAAGTTTTTATAAATATTGTAATTATACACCTGAACAC *F GCACACACACTTGCACACAAAAAGACAGGGTGAAGACCTACCAAAAAAAAAAAAAAAAAA *F translation M Y L *F R L A Q F T F I F S E T L A T S E A L K L Q V T N T *F I L S L S R S L T S T R P H I L V I *F \************A. mellifera BB260008A10B2.F *F GCTAAGGGAAAGGCGAAGTCTCGTTCCAACAGAGCTGGTTTGCAATTCCCTGTTGGTCGTATTCATAGACTTCTTCGCA *F AAGGAAATTACGCAGAACGTGTCGGTGCAGGAGCACCAGTGTATTTAGCAGCCGTTATGGAATATTTGGCTGCTGAAGT *F GTTGGAATTGGCGGGAAATGCTGCTCGTGATAATAAAAAAACCAGAATTATACCACGTCATCTTCAACTTGCTATCCGT *F AATGATGAAGAATTAAATAAATTACTTTCTGGAGTAACTATTGCTCAAGGTGGTGTTTTACCAAATATTCAAGCAGTTT *F TATTGCCAAAGAAAACTGAAAAAAAAGCTTAACCATATAAACATCGATATAAATGGCCCTTTTTAGGGCCACAATTTTT *F TAAAACGAAGGAATTTCTTATCCGATTCATAATTAAATAAAATACATTTTTATATAATAAAAAAATATAGAATAGAGAA *F GTATAAATAATCTAAAGATATATTATTCAAAGATAGAATTAAATATATTATTGAAAAAAAAAGATATGATATAGAAAAT *F TTCGTGAAATCATTTTTAATATAATAAAAGCAATATATTTTTATATTATTAACTACAATTTAAATCATACATATCTATT *F TTCAAAAATTTTATATTATAAGTTATTTTATAAATATAATATATTATTATTTTTCTTTTTTGTTAATTATTAAATATTT *F GTAATTTTCTTTATATCTAATAAGACTAGTAAATATAACATAAGAATATTGGACTATTAGATTCGTAAATTGCATTATA *F AAATAATAAAAATTATATTTAGTCTTATACCGTATATTTTTTTTATACG *F This encodes the end of Histone 2A, but genomic match is not annotated because *F it is a smallisolated scaffold with the N-terminus truncated! *F And there is something else wrong with it because it does not encode the *F C-terminus \- indeed the ESTs show there is a deletion in the genomic DNA. *F There are many ESTs *F AE002735.2 *F TGAAGGGAAAGGCAAAGTCCCGCTCAAACCGTGCCGGTCTTCAATTCCCTGTGGGCCGTATTCACCGTTTGCTCCGGAA *F GGGCAACTACGCAGAGCGTGTTGGTGCAGGCGCTCCAGTTTACCTAGCTGCCGTAATGGAATATCTAGCCGCTGAGGTT *F CTCAAGTTGGCTGGCAATGCTGCTCGTGAGAACAAGAAGACGAGAATTATTCCGCGTCATCTGCAACTGGCCATCCGGA *F ACGACGAGGAGTTAAACAAGCTGCTCTCCGGCGTCACAATTGCACAAGGT----------------------------- *F -------------------------------------------------------------------------CGTCAA *F TCAAACCGTCCTTTTCAGGACGACCAAATTATTAGCAAAGAATTGAAAAAAATTTTAACCACGCAATTTGTTGTATAAT *F ATTAAATCATACAAAAAATATTTCAAACTATTTATTTACGTAAAGATTGTAATATAATACGGTTTTTGTATTTTTTCTA *F TTATATGCGGTATAAACTATAATTTGTTTCTTTAATTACTCACACATTACTCTAATTACTAATTAGATTACTCTCCAAT *F TATAATTACTAATAAATTACTCTCCACAAATCAATGCTAGGAATACACCTTGGTATACCTGAAGGAGTACGAACGCCGG *F ACATTTATCATACGCGTTACTTTTAGAGTAAAAGGGTATACTAGATCAGTTGAAAAGCATGTAACAGGCAGAAGCCCCA *F CCGCTATCGCCCTGGAACCGTGGCCTTGCGTGAAATTCGTCGCTACCAAAAGAGCACCGAGCTTCTAATCCGCAAGCTG *F CCTTTCCAGCGTCTGGTGCGTGAAATCGCTCAGGACTTTAAGACGGACTTGCGATTCCAGAGCTCGGCGGTTATGGCTC *F TGCAGGAAGCTAGCGAAGCCTACCTGGTTGGTCTCTTCGAAGATACCAACTTGTGTGCCATTCATGCCAAGCGTGTCAC *F CATAATGCCCAAAGACATCCAGTTAGCGCGACGCATTCGCGGCGAGCGTGCTTAAGCTGACACGGCATTAACTTGCAGA *F TAAAGCGCTAGCGTACTCTATAATCGGTCCTTTTCAGGACCAAAAACCAGATTCAATGAGATAAAATTTTCTGTTGCCG *F ACTATTTATAACATAAAAAAAAATAAGAGAACAAAATTCATATTCTATTATTTATGGCGCAAATGGTACTGGGTCTTAA *F ATGTAAAAATAGTAATTCTTTCAGAGAAAGAATCAAAATAATCTT *F ESTs *F GGCACGAGGACTAAGTGAAATAAACGCAAAGCAAAATGTCTGGACGTGGAAAAGGTGGCAAAGTGAAGGGAAAGGCAAA *F GTCCCGCTCAAACCGTGCCGGTCTTCAATTCCCTGTGGGCCGTATTCACCGTTTGCTCCGGAAGGGAAACTACGCAGAG *F CGTGTTGGTGCAGGCGCTCCAGTTTACCTAGCTGCCGTAATGGAATATCTGGCCGCTGAGGTTCTCGAGTTGGCTGGCA *F ATGCTGCTCGTGACAACAAGAAGACTAGAATTATTCCGCGTCATCTGCAACTGGCCATCCGCAACGACGAGGAGTTAAA *F CAAGCTGCTCTCCGGCGTCACAATTGCACAAGGTGGCGTGTTGCCTAATATACAGGCTGTTCTGTTGCCCAAGAAGACC *F GAGAAGAAGGCCTAAACGTTTCAAAGGCTAAGCTAAAAACCTACATGTACATAAAATCGTCAATCAAACCGTCCTTTTC *F AGGACGACCAAATTATTACCAAAGAATTGAAAAATTTTTTAGCTTGGCAATTTCTTGTA-ATTAGTAAATCATAAAGAA *F TTATTAACGTAAA *F Histone 2A M S G R G K G G K V K *F G K A K S R S N R A G L Q F P V G R I H R L L R K G *F N Y A E R V G A G A P V Y L A A V M E Y L A A E V L *F E L A G N A A R D N K K T R I I P R H L Q L A I R N *F D E E L N K L L S G V T I A Q G G V L P N I Q A V L *F L P K K T E K K A \* *F \**********extra There are some weaker matches to this histone and they are *F not annotated either *F This one is to a 15kb scaffold with just one protein annotated at the front *F No ESTs for this clear histone relative \- not sure why *F AE002870.2 *F tattgccccacaagcttagccgaaaaATGTTTCGGTCACATTCCCTCCTCTTCACTTGGTGCAAAATAAATTGCCGGTG *F CCGGTCTTCAATTCCTGTGGGCCGTATTCACCGTCTGCTCTGGAAAGGCAACTACGCGTGTGGGTGCAGGCGCCCCAGT *F TTACCTAGCTGCCGTAATGGAATATCTGGCCCTGAGGTTCTCGAGTTGGCTGGCAATGCTGCTCGTGACAACAATAAGA *F CTAGAATTATTCCTCGCCATTTGCACCTGGCCATCCGCAACGACACGGAGTTAAACATGCTGCTCTCCGGCGTCACAAT *F TACACAAGGATGCTCTCTGTTGCCTAAAAAGTCAGAAAAGAAGGCCTAAACGTTTCAAAGGCTAAGCTAAAAAACAACA *F CGTACATAAAATCGTCAATCGATGC *F translation M F R S H S L L F T *F W C K I N C R C R S S I P V G R I H R L L W K G N Y *F A C G C R R P S L P S C R N G I S G P E V L E L A G *F N A A R D N N K T R I I P R H L H L A I R N D T E L *F N M L L S G V T I T Q G C S L L P K K S E K K A \* *F \*********** Check out the rest of this 15kb, and in the first half above *F histone relative is all there is with BLASTX matches, and no ESTs for it *F But the second half has two long 1kb long ORFs that must encode something, on *F the opposite strand to the histone gene *F But it's just a boring LTR retrotransposon! *F \**************A. mellifera BB260007B20F9.F *F AAATCATGTCGATTACTCGCGCACTTAAGTAGCAATTAATTAATATTCGTATATATATCCGATCCGATTTTTTTAGAGC *F ATGATAATGTACCGTGAATATGCTCTAACACTGAAATGTGAGTTTGAATGTGTCGAGTGTCCAAGGAATTTTTTTTTTT *F TTTCAAAAAGAACACGTGCGTGTGATTGTGCGGGGAGGAAGACGGGATGACCACTGGCTTCTTGTCTTCTTCTGCCCTG *F CGTTGCGCGTGAAATGGTAAGCGTGAATGTAGATGCGTGACTGTGGACGAGTGATTCTGACGGGAGAGACACTGCGAGA *F GTTGTTTTTTCTCTCTCTCTCTCTCTCTCCCCCCCTCCTCCTTTTCCTTTGTAACTATTGTTCGCGATCGATCCCGCAA *F AAGTATGTTACGTAATAAAAGTATCTGGCACATTTTCTTTCAGTTACTGGACACCCTCCCGGTGTGCCAAGATTTTAAT *F CGACAAGTGTGCAACCGTCCCGCCTGCAAGTTTATCCATCTCAGTGACGGAAACGTGGAGGTGATCGAGAATCGCGTGA *F CCGTGTGCAGGGACGCACTGAAGGGCGCGTGCATGCGACCCCAGTGTAAATATTATCACATACCGGTCGCGTTGCCGCC *F GGCGCCCTTGATGGCGATCACGTTCCCTGCGACGCCCTAATTACTCCTTCTCGTTTAGCGGATGTGTGCATCACGACGA *F GGAGAATAGAACACCGGCCAACCGATGATGATGAACGCGGAGAGAAAATTTTGACAGGGATCGAAAGAAACGAGGATCC *F AACCAAGGAATTAATTGCCCAAGAAAGCATCGC *F This appears to be an unspliced transcript with an exon in the middle. *F It has genomic matches e-21, but BLASTX only e-08 and to vertebrate *F equivalents of muscleblind B isoform *F Turns out the current annotation is incorrect near the C-terminus, leaving out *F at least one exon. *F It is a massive 100kb gene, with huge introns, and I can't find anything in *F them! *F \************A. mellifera BB260003B20D12.F *F AAATCCTAACAGTTCTCATGTACTTACGGAAGATACTATATCGAGAAAAGTTAAAAATGGTATATTATATAGCACACGT *F CTTTTGACAAAAACTAATAGAGTACCTAAATGGGGAGAGAGATTTGTTAGCAAAAATATAGTAAAAATTATTGAAGAGA *F GTATAGTGGATCCAAAAACAAAAACTTTAACAACATATACAAGAAATTTAGGTTACACTAAAGTCATGAGCATTGTAGA *F GAAGGTTGTTTATAAAGTATGTGAAGAAAACTCTAATTGGACAGTAGCAAAACGATCAGCTTGGATTGATAGTCAAGTA *F TTTGGATTCAGTAGAGCTATCCAAGCATTTGGATTGGATAGATTTAAAAAGAATTGTACTCTGATGTATAACGGGTTTA *F ATTACGTTCTAGCTCATTTGTTTCCTCACACAGCACAATATATGAATCCATCGCTTTCTCAAATGGGTTTTGCTCATCT *F AGTCGAAGAATTTCCTGGAAAGACAAGTTTAGCAGAAGATTTCCAACATTCGTTACAAGGTAAAGCAGAAAAAGTAAAA *F GATGCTGCGAAAAAGGCAACTGATTTAGCAAAGAAAAAGGCTGGCACCATTTATGCTACATAACATTCTGAGCAATCAT *F AATTAAAACAACATCGTCGGTGTGAATGATTTAAAATATTTGGCGAATAGAGAAAATGAAAATTCAGAAAATAATTTTA *F AAATGGAAACAAAGGATAATTATTTGATGGTTTATTGGATGAGTAAATAATAGAATGAAATTGTTAGCGTTATTCGTTA *F AAAAATA *F This one is barely better in TBLASTX, but the human BLASTX is much better, and *F indeed find the annotation of CG8806 needs fixing, I think to remove an exon *F \************A. mellifera BB260004A10B10.F *F TCAACAAACTATTCCATCGAATATGAATCATAACTTTCTTCAGAGCCTAAAGATGGCAAATCAATTTTAAATCGATATA *F TGAAGTTGTACTCGTCTGCCATCTCGTGGCGCTACGTAAAAAATATTTATCGTACCGATATAACATGGCGTTCACGTTG *F TGGGCACTTTTCGAGGTAACCGTGTTATGTTTGAATGCGGTCTGTATTTTGAACGAAGAGAGATTTCTTGCAAAAGTTG *F GCTGGGCATCGTGGCAAAATGTTCAAGGTTTTGGAGAAACTGCTACAGCTAAATCACAAATTTTGAATCTTATTAAATC *F GATACGAACGGTAGCACGAGTTCCATTGATATTTTTAAATATCATAACAATAATTGTGAAACTGGTGCTCGGTTGAAAG *F AAACAATTGATGATGTAAATAAGAGGATAATGCACAGTGAAAAGTCAAAGCGTGATGTACATAAATGAACAATTAAATT *F CTTTTATTTTTATCGCTGCATTAAAAAAAAAAAAAAAAAAAGCAAC *F Shows that CG6316 needs to be split into two genes. *F The C-terminus matches this cDNA, which is full-length and encodes an 80aa *F protein with excellent matches to similar length human and yeast proteins *F \***************A. mellifera BB260004A20B3.F *F TTTCTTCTTAGATTTGATACTAAATAAAGAACTTAAATTGCATAATTTCAATTTATATAAATGACTATGCAGACTTTAA *F ATTGTGTGATGAAAGTGATTTTGAACTATATATGTTTTTTTAAGTGCGCTTCTTATAGTGAAATTAAAGCATAAGGTGA *F TAATTAATAATTAATCAAAATGGCTAACATTCAACTTCGTGAATTAGAAGAATATTTACAACAGTTGGATGGATTTGAT *F AAACCAAAAATATTACTTGAACAATATTGTACTAGTGCTCATATTGCATCACGCATGTTGTACTGTGCTGAAGTTCAAT *F TTAATGACATAGAGGGACATTCAGTAGGTGACTTAGGTTGTGGGTGTGGTGTTTTATCACTTGGGGCACAGATGCTTGG *F AGCAAGTCATGTAATTGGTTTTGAAATAGATTCTGATGCACTTAAAATTCAATCTAAAAATTGTAATGAAATAGATTTG *F TTTGTGGAAACTGTACAATGTGATGTATTACAATATTTACCAGGCCGATTTGAGAAGTACTTTGATACAATTATTATGA *F ATCCACCATTTGGTACAAAGCATAATACAGGTACAGATATGAAATTTTTAAAAGTTGCAACCAAATTAGCATCAAATAC *F AGTGTATTCATTACATAAGACAAGTACCCGTAACTATGTTCTTCAGAAAGCTGCACAATATGGAGCCAAAGGCAAAGTT *F ATTGCAGAACTGAGATATGATTTACCAAAAGCATATAAGTTTCATAAAAAAATGTCTGTAGATGTTCAAGTGGATTTTA *F TACGATTTGAATTAAATTACTAAATATTTTCATGAAAAAAAA *F Shows that CG9666 needs to lose its N-terminus and gain a C-terminus by adding *F a simple exon. Human and C. elegans proteins confirm this new structure. *F cDNA LD25448.3prime confirms this *F \***************A. mellifera BB260004A20G4.F *F TCATGTGTGAGGATACAGTGACAGCTGTGGACAGGTACGCGTTTTAAATCTGTTATGATTAAAAACGTACCAAGTAAAG *F CCGGTCATCGACCAGCTGTGAATTCAAGAAAGAGATCGTTGTAAACGCGCGTGTCGAAAATTCTGTGAACGTGCGTTTT *F GATTTATCGGTTTATTACGCCTAGTCGAAATCGTATTACCGAAAAGAGTCTTACCGAAAGAAACGGTGCGTGTAAGAAC *F CTTTTTGGATACTACCTGACAAGATGTGGTGTCTCGTTAGTCAAGCTAATTCTGTCATTCTTGAGGTACAAGTCGATCC *F CAAAGCTATTGGTCAGGAGTGTCTCGAAAAGGCATGCGATTGTTTGGGCATTAGCAAGGAATGCGACTACTTTGGGCTG *F AAGTATCAGAACGCGAAGGGCGAGGAGCTCTGGTTGAATCTGAGGAATCCTATAGAGAGGCAAACGGGCGGCGGTGTGG *F CCCCGCTAAGATTCGCATTGAGGGTTAAGTTTTGGGTACCGCCTCACCTGTTGCTGCAAGAAGCTACCAGGCATCAATT *F CTACTTGCACTCTCGCCTCGAGCTTCTCGAAAGTAGGCTAAAAATGGCGGATTGGAGTTCGGTGGTGCGTCTGTTTGCT *F TGGATAGCGCAAGCCGATATTCGTGATTACGATCCATTGTCGCACCGAACGCCCTCTTCTTGCATTGCTGTCCAATTCA *F ACGGCGGAAACAAGCGAATCAAACCGTTGGTCTTATTCACCGGATGGTCCACCACCCAAGAATTGAAGGGAAGAAGCCT *F TCCCGGG *F This suggests the annotated N-terminus of CG12489 might be wrong, since the *F honey bee sequence nicely matches the N-terminus of human ortholog, yet cannot *F find better N-terminus in Drosophila genome? *F Drosophila cDNAs agree with current annotation, so may be real difference *F between Drosophila and other insects *F \***************A. mellifera BB260008B10H2.F *F GCGAGCGGTGGGACGGGCGGGTACATCGCGCCTACCACGTGGGATCAGCTGATGCAAGACGATCATTTCCTCGGCAAAT *F TCTTCCTCTACTTCTCCGCCATCGAGAGGAGGATTTTGGCTCAGGTATGCTTAAGATGGAGAGACATACTTTACGCGCG *F GCCTCGACTTTGGGCAGGCTTGGTGCCCGTAGTAAGATGTCGCGAGGTACGTGCCATGCCTTCTACTTCACGCACGCGG *F CTCTACGCCTCCTTAGTTAGAAGAGGATTTCATTCGTTGGTTCTTCTCGGAGCATCGGACGAGGATATCCCGGAACTGA *F CGCACGGCTTTCCATTAGCGCAAAGAAATATTCACTCGTTATCGTTGAGATGTTGCGCGGTGACCGACAGGGGACTAGA *F AGCTCTCTTAGATCATTTGCAAGCGTTGTTCGAGCTTGAACTAGCAGGTTGCAACGAAATAACGGAAGCCGGGTTGTGG *F ACTTGCTTGACACCTAGAATAGTATCGCTCTCCTTGTCGGATTGTATCAACGTGGCTGATGAAGCCGTCGGTGCTGTCG *F CTCAATTACTGCCGAGTCTCTACGAGTTCTCGTTGCAAGCTTATCATGTAACCGACGCCCGCCTTGGATATTTTCACGC *F AACCCAGAGCAGCTCCCTTAGCATCTCAGGCTGCAGTCCTGCTGGGGACCTACCCACCATGGTATAGTCAATATTGTAC *F ATTTCTTGCCCAATCTGACTGGTCTATCGGTGGCCGGATGCAGCAAAGTAACCGAGGACGG *F Something very strange here. An excellent human match is recovered, as is a *F TBLASTX to Drosophila genome, but can't get a BLASTX match to Drosophila *F protein; *F yet there is a protein annotated in this region, CG6060, but the annotation *F doesn't quite match \- check it out *F Matches entire human protein, but clearly is longer at N-terminus at least. *F Weak full-length match to Partner of paired , from 170 of 550aa *F 225198RC-ATGGAGAGAGCACGGGGAAAGCCACGGGTCGAAAATCGATACTGCTGCCCAGGCAGCACGCAGGCTTACG *F GATACGGACTCTCCACCCGGACCCTGGAACCCCGGATTCGGGCGAAGGCGgtgcggagtgcattatggcaatgtggag- *F 2822-ccccatggtgtatcgtgttgcctcgttacagGTGAAACTCGCTAATTGTCGGGTGGAAAAAGgttagcaataca *F cagagcaaacgggatgtaaaggataagcaaagccgacaccataagcgattgaaaaacgaagcagaggagaggacgaagg *F actccacttttccagagcgcttcgtttcccagGAGGCAAGGTGAATCTGTTCCAGCACACGATGTCATCGATCTCGGCG *F CAGGGCGTGGTCGAGCGAGCATCCGCGGAGTTGTCGAAGCGGATCAATGGCCTGGGCCTGCGCTCGAAGCACCATCATA *F GCAGCACATCCAGTGGTGCTGGTGGCGCCGGCGATGCTGCATCCCCGGCAGGAGCCACGCCCACTCCGGCAGCGCCCAG *F CGGCAAGACGTCGGTGATGGAGCGCGTAACGAACGCCCTGTGCGGCGGTGGCAACTCAAATTCTAACTCAGGATCGAAT *F AGCTCCAATAGCAACACCTCTTCAGCGTCTGCCACCGCTGCCACATCGCCCGCCAGCAACGCCAATCCTCCACAGACGC *F CGGACAAACCGTCGCGTGGCAGTAGCCCCAGTCCCGGCGGTATCACAATGCCAGgtggccagtcgcaggtccagaactc *F cacacaccacctcctgcagcagcaacaacagcaacagcagcatatgcagctgcaacaatcgcagcagcagcatctccag *F ctgcaagcctccacgctgatcaactccaaccaccatgtgatggtgggtcctgctccgcccactggcatgcctctgggtg *F ccccgcccacgccgacagtgaagtccattgccaagcagatgaacataaccataccggg *F CG6060 M E R A R G K P R V E N R Y C C P G S T *F Q A Y G Y G L S T R T L E P R I R A K A *F \-------------------------------0--------------------------------- V K L A *F N C R V E K *F \----------2----------------------2----------------------2--------------------- *F -2----------------------2--------------------G G K V N L F Q H T M *F S S I S A Q G V V E R A S A E L S K R I N G L G L R *F S K H H H S S T S S G A G G A G D A A S P A G A T P *F T P A A P S G K T S V M E R V T N A L C G G G N S N *F S N S G S N S S N S N T S S A S A T A A T S P A S N *F A N P P Q T P D K P S R G S S P S P G G I T M P *F \-------------------------------1---------------------------------------------- *F -----1---------------------------------------------------1--------------------- *F ------------------------------1------------------------------------------------ *F --- G R S K S R F A H L Q H H G H G G R P E G G G Q C *F W R Y T V A V A E T T A Q S S P A *F PSVWQYGHQRSIAADHATPAPPAAYPSMSTRSVCAAPRLATGDWRPCLTTCRVCLNWSWLAATR *F I can't reconstruct this gene with their coordinates, so leave it up to them *F to sort out. *F \************A. mellifera BB260008B20A1.F *F AAGCGCAGCTAGGACGTCTCCAGTACGGTCGACGCCCTAGTTCCCCTCCGCATGGAGGGAACAGGCGCGCACCCCGAGG *F GCCAAATCTACTTCCTCACCCCCTGACGCGACCACGACATGGGAAGATCCACGAAAAACAGCGGCGGCGGCGAACGTTG *F CGGCTGTGGCCGCAGCCGTCGACAATGGGAAATCCTCGACCGGCGCTACCAATTCTCTAGGTCCATTGCCCGACGGATG *F GGAACAAGCGCGTACTCCCGAAGGAGAAATCTATTTCATTAATCATCAGACACGCACCACTTCGTGGTTCGATCCAAGA *F ATCCCTACTCATCTTCAAAGGGCTCCGACCTCAGGTGCAATGTTACCGCAAAATTGGCTTCAACAGCAACAACCTACAG *F GTGGTGGTATTCAGAATAATCAAACATTGCAAGCGTGTCAACAGAAACTTCGCCTCCAGTCGCTACAAATGGAACGCGA *F GCGTCTCAAACAACGGCAACAGGAAATTATACGTCAGCAAGAGCTAATGCTTCGACAGAGCACCACCGACGCCGCTATG *F GACCCATTTTTGTCGGGAATCAACGAGCAACACGCACGCCAGGAGAGCGCGGACAGCGGCCTGGGCCTTGGTTCCGCTT *F ATTCCCTCCCTCACACACCGGAAGATTTTCTTGCAAATATCGACGATAATATGGATGGTACAAGCGATGGCGGCGCACC *F CATGGAGACCCCGGATCTTTCTACTCTGAGCGATAATATCGATTCGACCGACGATCTCGTTCCATCGTTACAGCTGAGC *F GAAGATTTTAGTAGCGATATTTTGGACGATGTGCAATCGTTGATAAACCC *F Has much better mammalian matches, and indeed indicates that there is at least *F an exon missing from CG4005 *F \************A. mellifera BB260008B20D1.F *F TCAACCCCTTGCGTGGTACCGATGTCATTTTACCGGAAACCGCAGTATTCGTAATAGCGCACAGCCAAGCTTGTCATAA *F CAAAGCTTCCACAACAGATTATAATTTAAGAGTTGCAGAATGTCGCTTAGCTGCACAGATGATAGCAAAGAAAAGAAAC *F AAACCTTGGGAACATGTACAAAGACTAATCGATATCCAAGAGAGTCTTAATATGAGCTTAAACGAAATGGTTTCAGTTA *F TAACAACCGACCTTCACGAAGAACCATATACCCTGAGCGAGATTAGCAAGAACCTTGATACAACGAATGAGAAACTTCG *F TGAAATATCATTATTACAAAATTTTAGCAATGCGCAAATTTTCAAATTGAAACAACGCGCTCTCCATGTGTATCAAGAG *F GCGGCTAGAGTGCTCGAATTCCAACATATTAGTGAGAAAAATGCAATTATGGAAGAGGAGAAGCTAAAACAACTGGGCA *F ATCTGATGTCCAACAGCCATTTCAGTATGCACAAACTATACGAGTGCAGTCATCCTAGTGTCAATTCACTCGTTGACAA *F AGCTATGGCTTGTGGTGCACTCGGTGCAAGGCTCACGGGAGCTGGATGGGGTGGCTGCATAGTGGCCATCATAACGAAA *F GACAAGGGTTCTCACATTGTGGATACACTGAAAAAAGAACTCGATCTATGCGGGATAAAGGATGGATTCAAGCTCCACG *F ATTTGGGTTTTCCAACGGAACCGAACCAGGGTGCTGCAATTTATATGAGCTAAGTTTCATTTTCATCTGTTCCTGGCTC *F TTGCATTTATAATTTCGACCATAATTATTAAAGGGTTCTAAGATATATTCTAATTTAAG *F The C-terminus of this and the mammalian orthologs indicate that the *F drosophila protein CG5288 needs to be longer, and the genome encode the *F appropriate sequence *F \************A. mellifera BB260008A20D9.F *F TTACGTTAATATTTGAATGAATAAATCGAAGAAATTTGGTTCATAAAAAATTTTTAAGACATTAATCGTGATGTGTCTA *F CAAATTATCCATTGATTTATTCGAAAAACTTTCGAGATATTCAACGGTCAGTTCCTATTAATAAATCGATTTTTCGCAT *F TCGAGTGAAGTTGCAGAGAGTTCGATCGATGGAACGACCAGAGGATTTAGTGTCGAAAGAAATGTGCAGAATGTGGTCA *F ATTTACAACGTATAAATTGTCTATCAGTTAAAACACGAAGAGCAAAATGTCGGTTGAAGTGAAGGGGGGTCGACCAACA *F ATGCCAACAATTCCACAATCCAAGAGACCAACCATTTTCGTTTATCCCACAGTAACTCCAGAGAGCATTATCATCCCGA *F TAGTATCATGCATACTCGGATTTCCATTACTGGCCCTTATGGTCATCTGTTGCTTAAGAAGAAGAGCAAAGTTAGCGAG *F AGAACGTGCACGAAGAAGAAATTGTGATCTAAATCATGGAACCCTTAGTCTCGGTCGTTTTAGCCCTGTTCACCGGTTA *F AGTAAATTAAACATCTTCTTTTTTATTTTAATACCATGTACATATCTAAAAAAAAGAAATGCAATTTATTTAAATTAAA *F TTTTACTTGAATTATTCCATCCTTACTACCACATTATGGTCGAATTAAAAATTAATGGTTAAGTCTTAAACTTAAAAGC *F CACGTTTTCCTACCAAACCTTTTTGTAACAAAAACACATGGAGGCCCCAAACAATTGGGTTCAACCCCAACCGCTAATT *F CGGTTGTTTTTCCCCTAAAACGCTGCTGACT *F Unspliced transcript has an exon in the middle, flanked by splice sites, with *F good match to unannotated region of Drosophila genome \- no BLASTP or EST *F matches *F 7kb in front and 3 after available *F agcctttcccttttaaatccatttcagTTTCACCCGAATCGATTGTCATCCCGATCGTCTCCTGTATCTTCGGCTTCCC *F CATCCTGGCGCTTCTGGTGATCTGTTGCCTTCGAAGGAGGGCCAAGTTGGCCAGGGAGAGGGATAGGAGGCGTAACTAC *F GATATGCAGGACCATGCCGTCAGCCTGGTCAGATTTAGTCCAATACATAGGCTTAGTGAGTTTGAATGTCGTAATGTAT *F TGTGTGTACGAAGAACGTTTCTTTTTCTCGTCTCGTCTCGTTTTCTTTTTTATCTCTGGTTTT *F V S P E S I V I P I V S C I *F F G F P I L A L L V I C C L R R R A K L A R E R D R *F R R N Y D M Q D H A V S L V R F S P I H R L S E F E *F C R N V L C V R R T F L *F There are several good looking splice sites and ORFs in the 3kb 3' to this, *F but can't easily put a gene together without any more guidance. *F Same for the 5' 7kb. *F \*************A. mellifera Contig1 *F GGAAAACTATTTTAACTGTCAAAAGAGAAATTTCGAACACATATTATATTATGTCTCATTCCACATCTCAGAATAAAAA *F CTACAATATTATTATTGGAGATATGCGAATTGCATACAAAGACAAAAACGAAACTTTTACAGAAGATCATCTTGTAAGT *F AAAGAACCAATTGGTCAATTTAGAGCCTGGTTCGATGAAGCATGCAAAATTCCGCAAATTTTTGAAGCAAATACAATGT *F TTCTTGCCACAGCTACCAAAAATGGAATCCCGTCCGTGCGACCAGTATTACTCAAAGATTATGGAGAAGATGGTTTCAA *F ATTTTACACTAATTATGAAAGTAGGAAAGCTCGCGAAATAGCTGAAAATCCAAATGTGGAGGTGAATTTTTACTGGCAA *F CCTTTACATCGAAGTGTACGTATAGCAGGTACAATAAAGAAAACTTCCTTAAAAGATTCAGAACGTTATTTTCAAAGCC *F GACCATATGCAAGTCAAATAGGATCAATGGCTAGTAAACAGAGTAGTGTAATTGCAAATAGAAATACACTTATAATAAA *F AGAAAGAGAATTGTTAGCTCAATTTCCAGAAGGGAAAGTTAAAAAACCAGATTGGTGGGGAGGATATATTATTATTCCA *F CATTCCATAGAATTTTGGCAAGGTCAAAGCGATCGCTTACACGATAGAATTCATTTTAGACGATTAAAACCAAACGAAA *F AAATCGACAATGTACTTGTTCAT *F Vertebrate matches are 260aa proteins, so suggests that CG2649 gene product is *F a fusion of two related genes, since first half matches well and second half *F matches more weakly *F \***********A. mellifera Contig1058 *F ACTAATCTTCCTTTGCCCCGAGAGCCGGAAATTAGCTATTGCGGGGAGCGGAAGGCACGTTGTCTTGTTCAAATTCAAG *F AAAGTAGAGAGTATGTCCGAGGTAGTGACTTTGGATATATCACTGACGGCCGAACCGGTTAAGGAAGTGGAAAGTTCAT *F CCGATCACGATTCTCCGGCTGGCGGCAACACTTCAGGGAGCAGCGAGTCGAAGAATAATGAATCGAGCCAATCGCTGAA *F AATTAAGACTGGTTTGCAGAAGAGGGCCGCGGGTTTCCAAGCGACCCTGGTCTGCTTGACGGTTACCAACAGCGGGGAA *F CAAGCTGAGAACATAACTGCTCTCAGTTTGAACTCTTCCTACGGTTTAATGGCTTACGGGAACGAGTGCGGCATAGTGA *F TAATAGACATTGTCCAGAAGATCTCTTTGATCGTGTTGAACACGGGCGACATAGGTGGTAACGTGGATCTGTGCCAACG *F GGTGCTGCGCAGCCCGAAACGTCAGGACGAGTTGAAACGGGATAACGAGGACAAAGCGAGGAGTCCTAGCACAGATCAG *F CCAACTATGTGTCTACCCACGTTGAAACAAGTTCAAATCAGTTTTGCGGTCTTCCCCGACAGCAAGGTTGATTCAGACA *F AATATGACAGTTCGTTTCAACGGTCAAGGAGCTCGTGCATGTCGTCGCTCGAGAACATCACCACCGAGACCATCAGCTG *F CCTTACATTCGCAGATTCCTACACGAAAAAGAGCGATACCAGCCCCGTGCCAACGCTTTGGATCGGTACCTCTTTAGGA *F TCTATACAAACGGTGATATTTAACACGCCGCCTCGTGGAGAACGACACGCGCATCCGGTCGTTGTTTCTACGTGCAACG *F GATCAACGTTCAAGTTGAAAGGATGCATTCTGTCTATGTCATTCCTGGATTGTAACGGGGCCCTGATCCCGTATTCCTA *F CGAATCTTGGAAAGATGACAGTATGGAAAGCAAAGAGCGCAACAGGAGTC *F Shows there are problems with annotation of CG17762; genome has exons for the *F additional matches, and vertebrate homologs indicate there are sections *F missing too *F \*************A. mellifera Contig1107 *F TTTTTTTTTTTTTTGTCCACTTATATTAGTGTTCAATTTGTTTAATAGTTATAAACGTTTGAATTTTCAGTGAATTTTA *F CTTATTTTTAGCAATTCAAAACTTCTAATGATGTCGAAGTTTTTATTGCTTCTTTGCTTTACTGCTGTCCACGTTCTAG *F GTGAAGACCAGGAAAATGTTTTATCTAAAAAGAATGATACTCTTCTCACTTCTCCCAGGACATCTTTACAAAATGATTC *F CGAATATTTAAAAGCAAATATTCTATCAAATAAGAATATTTCCATGACAGATTTAGGAATTATTTCAAACACAGAGAAT *F AAAATAAAGCAACAAGAAGTTATTAAAAATTCTCTTCAAACTTCTACAATTATTCCCAATCATTCTATTGTCATGCCAT *F TAGATATGACAGCTATTTCAATTTCTACAAATGAAACATCTAAAAAAATTTCGGATATAACTAATCCAATAGTTATACA *F TGCTCCAATAAATTCTACCTTGTCTTCTTACACAACTGGTAAATGGACAGTTGTTAATGGAACAGATCAAATTTGTATT *F GTAATACAGATGTCTGTAATGTTTAATATCTCTTATGTCAACATTAATAATAAGACATCTTTTATAACATTCGATATAC *F CAACAGATAATGTTACTACAAAAGCAAGTGGATATTGTGGAAAACTGGAACAAAATTTGACATTAGAATGGTCTGCTAA *F AAATATAACTAATGGTAGTATGACATTGCATTTTATGAGAAATGCAACTGAAAATGATTATTCTCTTCACCATTTGGAA *F GTCATTCTTCCAGCATCAGATTTTCCTTCAAATTTAAAACTGAATGGATCAGTATCTTTAGTACATGAAACACCTGATT *F TTGAAGTTAGATTATCTAATTCTTATAGATGTTTAAAACAACAAACACTCAACTTAAAACAGAATAATAGTAATGAGAC *F ATCTGGTTATTTAATTGTATCAGGACTCCAATTTCAAGCATTCAAAGTTGATAATTCTACTATGTTTGGTTTAGCCAAA *F GATTGCGCTTTTGATACACCAGACGTCGTACCAATAGCAGTAGGCTGTGCATTGGCAGGATTAGTGATTATAGTATTGA *F TCGCGTACTTGATTGGTCGTCGTCGAAATCAAGCTCATGGCTATCTTAGTATGTAATGTTAATATGTTTTTTATTTTTA *F ATTTTTTTCGTTGATTCAGTGAAAATTTCATTATTTAGTTTATATAATGTATTATAATGTTAGCTGCAAAACTTAAAAA *F AAGATATTTCCCAAAAATCATATATTAAAAATTGCAATAC *F Both this and the matches to vertebrates strongly indicate that the *F N-terminus, and certainly the C-terminus of CG3305 are missing ; a good *F C-terminus is encoded in the genome. *F \**********A. mellifera Contig1163 *F AGGAGTACGCCACCGCTGGATACTGCACCCCACATTGCACGCACACGATGTTCCCCGAGAGCGGAGTGAACATCGTTTC *F GGTGGTGCTGCACTCCCATCTGGCCGGTCGGCGGCTAAGCCTGAAGCATATCCGTCAAGGGAAAGAATTGCCGAGGATA *F GTGGAGGACAATCACTTCGATTTCGAGTACCAGCAGTCTCACACTCTGGAAAAGGAAGTGAAGGTGCTTCCGGGAGACG *F AGCTGGTGGCCGAATGCGTTTACGGCACTCTGGATAGAACCAAGCCCACTTTGGGGGGATACGCCGCTTCTCAGGAGAT *F GTGTCTCGCATTCGTGGTCCATTACCCGAGAACCCCGCTTGCCGCCTGCTACAGCATGACTCCGTTGAAACATCTGTTC *F AAAACATTGGGGGTGTACAGCTTCAAAGGCGTCACTATGGACCACTTGGAGAAACTCTTCCTAACGACCAGAACGGACG *F CAGTAACCATTCCTTCGACCGGCCAACAACAACTTCCTATCTACCCGGCAACCAGGCCTAGCGAGGACATCGACGAAGA *F GCTTATTAGGGAGGCCAAGTCAGCGTTGAGGGCCGTGAAGGATTACACTCTGGAGCAGGATAACGAAAATGTTTTCTCT *F AGATTGATCATCGAGGAACCGGAAGAGTTCAGAGGTCGAACTTTGGCAGAGCACATGCTGGCGTTACCTTGGACCGAAG *F AACTTCTGGCAAGGGCCATCGAGCAGAACCTGTACCACGGAAGGCACATGACTTTCTGCAGGAAGAGAGACGATAAACT *F CGCTCTGCCAGCAGACATACAAACGTTCCCTAATTACACGGAATTACCGGAAGCAAATGAAACGATGTGCACGGAAATG *F GCAAAATTATCCAATGCGTCGGGGAGGATGTCGTACCTCGATATCGCCACGTTCCTCGCG *F Identifies a 1500bp ORF at the C-terminus of CG13075 that needs to be included *F in the annotation *F \***********A. mellifera Contig1167 *F GCAATAGGCGAGGAAATTTTAGATTTATCTGCTATTGCGCATCTATTCGATGGACCATTATTAAAAAACAAGCAAGATG *F TATTTCGTCGTGATTATCTCAATGATTTTATGGCCTTGGGAAGATCCGCTTGGATAGAAGCCAGGAACAAACTTCAAGA *F CTTATTATCAATCAGTAATCCAACCTTGCAGGAATCTAATATTCGTTCAAATGCCTTTGTAAAACAAAATGAAGCAACA *F ATGCATCTACCAGCAAAAATTGGTGATTACACAGATTTTTATTCCTCGATTTACCATGCTACAAATGTGGGCATCATGT *F TCCGTGGAAAAGAAAATGCTCTGATGCCAAATTGGAAACATTTACCAGTCGCTTATCATGGAAGAGCGAGTTCAGTGGT *F CGTTTCTGGAACACCGATAAGAAGACCTTTAGGTCAAACAGTTCCGATAGAGGATGCAGATCCAGTTTTTGGCCCTTCA *F AGATTAGTAGACTTTGAATTGGAAGTAGCTATCTTTGTCGGAGGACCACCTACAAATCTAGGTGACGCTGTTCCAGCAT *F CCAAAGCTTACGATCATATTTTTGGAATGGTTACTATGAACGACTGGAGTGCAAGAGACATTCAAAAATGGGAATACAT *F TCCATTGGGACCTTTCGGTGCAAAAAATTTTGGAACTACTATTTCTCCATGGATAGTCACTATGGAAGCTCTAGAGCCT *F TTCAAAGTGCCCAATGTGCATCAAAATCCAACCCCATTCCCCTATTTACAACACAATGAATCTTGTAACTTTGATATTA *F AATTAGAAGTTGACATTAAATCTCCAAACGGTACCGTCACAACCGTCTGTCGCAGTAACTATAAATTCCAATACT *F Annotation of CG14993 needs fixing; N-terminus is missing, shown in a nearby *F exon by this transcript and the mammalian proteins. *F \**********A. mellifera Contig2454 *F ATTCTGATCCTAGCCAACAAGCAGGATCTGCCAGGTGCCAAAGAGGTGGGCGAATTGGAAAAGCACCTGGGCGTGCTGG *F AATTGGCGGGGATGCCGGGGAGCGCGTGCATCAGGGTGCAGCCGGCCTGCGCGATCACCGGCGAGGGGCTTCACGAGGG *F TTTGGACACTCTTTATCAGCTGATACTGAAGCGGCGCAAGCTCGCGAAGCTGAACAGGAAACGGGCCAGGTAGGCCAGG *F GCCTCGGAGGACTGCACGTGCGTCTTCTGATCTTGCAAGTCGCGGACAGTCTCTCCTTCGGGCACAGCCACGCCTTCCA *F CGGTGTCTTCTTCCTCCCCGTCACGATGCCGCGGCGCCGACCCGCTCGAGTGAACAACGGAGCTGCGCGCGCATCCAAC *F GTCTCCACGAACATTGCCCACTTCGCCGTGGACGAAGTCGTCTCGTCCGTTCCAACGTAGTTGGAATCTCTGTTGCGTC *F GCGTCGAAGATCTTTCCAAGCTTTCATCGATTCGTAGAGGATCTCCTCTTTATTCCTCTCGATCGGGAGATCTCGGTTC *F ATCGAGTTTCGGATGAACTCGAAACAAGGATGGAATTTGGAACGGGCGCTTTTTTAACGCGCGAGGAGGAATGTCGAAC *F GGGATATCCCTCTCCGCGAAGAGGAGGATAAAATAATCTAGGA *F Matches region annotated as CG2219; yet does not show up in the translation? *F \************A. mellifera Contig2801 *F TTTTTCTTTAAGTAACAATAAACATGACAACAGCACTGTATTTAGAACATTATTTAGACAGTTTGGAACATCTACCTAT *F TGAATTACAAAGAAATTTCACTTTAATGCGAGACCTTGATGCTAGAGCACAAGGATTAATGAAAGATATAGATAAATTA *F GCAGATGATTATTTAAAAAATGTAAAGAAAGAATCCCCAGAAAAGAAAAAGGAACAATTGACTCATATTCAAAACTTAT *F TTAACAAGGCAAAGGAATATGGTGATGATAAAGTACAATTAGCAATACAAACATATGAATTAGTTGATAAACATATTAG *F GAGACTAGATTCTGATTTGGCTAGATTTGAAGCTGAAATACAAGATAAAGCTTTAAATAGTAGTAGGGCACAAGAAGAA *F AATAATGCTAGTAAAAAGGGCAGGAAAAAATTAAAAGAAAAAGAAAAACGAAAGAAAGGTGCAGGTACTAACAGTGAAG *F ATGAATCGAAAACAGCTAGAAAAAAACAGAAAAAAGGAGGATCTGTTGCTTCTGCTTCATCAGCTGGAGCTGTAGGAAG *F TGGTGCTCAAGTAGATTCTACTGCACTTGGTCATCCAGCAGATGTTTTAGATATGCCAGTTGATCCTAACGAACCAACT *F TATTGCCTATGTCATCAAGTTTCTTATGGGGAAATGATAGGTTGTGATAATCCAGATTGTCCTATAGAGTGGTTCCATT *F TTGCATGTGTTC *F Encodes excellent match to N-terminus of CG9293; but alignment, along with *F vertebrate matches, and confirmed by cDNA LD46333.5prime, shows two and maybe *F three introns that need removing. *F \***********A. mellifera Contig379 *F TTGTAATCGAAGTAAATATCGTGAGTTATTCGTTTCATTTTACGGGAGAAAAAAAATTTTCTCCTAACAGTGTCACAAT *F GCTACAATCGTTAATCTAAACAAACTATAAAGAAAGGGGAACAAAGATGATGTTTTGCTGTTTGAGAAATTGTTTTGAC *F GGCCTTGGCTTTGCCGCAACTCAAACACCGAAGAGAGAACCAAATCCTATATCTTTAGACACGTCTTATATGGGACATG *F AAGTTGTGATAGTAAAAAATGGTCTAAGAGTATGCGGTCGTGGTGGTGCCTTAACAAATGCTCCTCTTGTCCAAAATAA *F AAGTTATTTTGAAATAAAAATACAACAAGGTGGTATATGGGCTATTGGATTGGCTACAAGATCCACAGATCTCAATATT *F ACTATTGGAGGAAATGATAAAGAAAGTTGGGCTCTTAATTATGATTCTATTATAAGGCATAATCAACAGGAAATACATA *F AGATTCAAAGTTCGGTTCAAGAAGGAGATATTATAGGCGTATCTTATGATCACATAGAACTTAATTTCTATTTAAATGG *F AAAACCAATAGGTGCTCCAGTAATGGGCATAAAAGGAACTGTTTATCCAGTACTTTATGTGGATGATGGGGCCATTCTA *F GACTTAATTTTGGATAATTTTATTCATCCTCCACCTACAGGTTTTGAAAAATCATGTTGGAGCAATCATTACTCTAGCA *F AAAAATATTATTACATAAGTATCAATCTTTTTATTCCTTGACACTAAAAGCATT *F This, and homologous human and C. elegans protein, strongly indicate that *F there are at least two in-frame ORF introns retained in the annotation of CG7785 *F \************A. mellifera Contig400 *F GAAAAATAAACTCAGGCCTAACAGTGGAAATGGTGCTGATCTGCCTAATTACAGATGGACGCAGACACTTCAGGATTTG *F GAGATCAAAGTGCCTTTGAAAGTGAACTTCTCAGCCAGGCCCAAGGACGTGTCGGTGACGATCACGAAAAAACGATTGA *F CCTGCGGCATCAAGGGTCAACCGCCGATCATCGACGGTGATTTTCCACACGAAGTCAAAGTCGAAGAATCCACCTGGGT *F GATCGAGGATGGAAAAGTGTTGCTTCTCAACCTGGAGAAGGTGAACAAAATGCAATGGTGGGCTCACGTGGTAACCTGC *F GATCCGGAGATCAGCACGAAGAAAGTGAACCCCGAGCCGAGCAAGCTTTCCGATCTCGATGGTGAAACTAGAGGCCTGG *F TGGAGAAGATGATGTATGACCAGAGACAAAAGGAACTGGGTTTGCCAACGTCCGACGAGCAGAAGAAGCAGGACGTGAT *F CAAAAAGTTCATGGAACAGCATCCAGAGATGGATTTCTCCAAGTGCAAGTTCAATTGAAATTCCAATTAGATAGGGGAG *F CAGCCGAATATTCAAGGCGTTGTTGTGAAACTGATAATACAAATGATAAAACAGATGATATATTATCGATATATCCAAT *F CCAGAAAAGCTCTTTATGATACTCTCTTGTTAATTGTCACCGCGGCGAAGTTTTTCTGCACCTACTTTATCGTATCGTA *F TTCCAAAGCGTAAAAGATGGCGCCACGATCCATGCCACGATTGAATCG *F This, and vertebrate matches, suggest there is an intron retained in CG9710 *F \***********A. mellifera Contig58 *F CCGCGTATTTTTAATACAATTGTTACAATATCGTTTTTTTCATTGTGGAAATAACGAATTTTCAACATGGTGCTAAGTG *F AAGTGAATAAATTTCTTCATGAATTAGAAAAAGCTGAATTAGAGGCGCCTGGTGGAGTTGCATCATCACAAACTTATGC *F TCAATTATTAGCTGTATATCTTTATCAAAACGATCTATGCAATGCCAAATACTTGTGGAAGCGGATACCAACGGATCTG *F AAAAGCGGAAATGCAGAACTTGGTCAAATATGGATGGTAGGACAGCGTATGTGGCAAAGAGACTGGCCTGCAGTTCATG *F TCGCCCTCAATGCAGAATGGAGTGAAGATGTTTCTGATATTATGGCTGCTTTGAAAGATAATGTTCGAGAAAGGGCAAT *F CACCTTAATATCAAAGGCTTATTCTTCACTAAGTTTAACCGTATTTGCGTCAATGACAGGCTTAACATTAGAGGAAGCG *F CGTCGTGTAGCAATTGAAAGGGGTTGGAACGTAGATGGAACGATGGTGCAACCTTGTAAGATTCAGAAAGAAGAGAGTA *F ACCTCGTGAACGAGGTGTGTCTTACTGAGGATCAGCTGTACAAACTCACTCAATTCGTGTCTTTCTTGGAAAACTGAAC *F AAGCAATGAAAGTCATCAATGATGAAATTCACGCAACACAAAGACACGATCGACAGTACTTTAAACTTAGTT *F This and vertebrate matches show that the N-terminus of CG13383 is missing, *F and it is there in the DNA separated by an intron. *F \************A. mellifera Contig622 *F AAAAAATTCGTTGAGCGGTGAAAAACCAAAATGAGAAACTCCTAATGGACCAAAGAAGGACGTGCTTATGCTGTTACTG *F CTGGGATGACTATGCCTTTGTGATCCAAGACGCACACCAACTCCAGCAAGGTCAGAAAATAAATCTTCAAAAGATGAAC *F CGCCAAAGAATTCTCTAAATACTTCTTCAGGATCCCTGAACATGAAAGTACCAGCAAAGTGTGGATCAAAGTCTTCCTT *F ATGTCGCCTCTTGCCACCAGGCATTTGAAGTCCTTCCTTTCCATATTGGTCATAAACCCTCCTTTTCTTTTCATCGCTT *F AGCACTTCATATGCCTCAGATATTTCTTTAAATCTCTTGTTTGCTTCCTCCAAATTTTCAGGATTTTTATCCGGATGCC *F ATCTCAACGCCAATTTTCTATATGCTTTTTTGATATCTCCGCTCGTGGCGGTTCGCTGCACTTCTAGTACCTTGTAATA *F GTCAACCATCGTTCACAATATTCACGCTCGGATGTTAGGGCTTAGGTAACCACCTAGAAGTGGCTCCTGCTTCTTCTTC *F GCCGTTCACGCTC *F CG8448 is annotated for this ortholog as mRNA, but not translated? *F \***********A. mellifera Contig78 *F GAAACATCAACAACTCCAGAATATGCTCAGGGATTAACTTCAATTAATCCACCTGCTACCCCCATTACTCCTGTAGCAT *F CTGTACAATCTTATACACCTACTACTCCAAGTGGAGTAGTACCAGTAACAACACCACAAACTCCTACAACACCAAGTAC *F ACCTACAAATCCGAGTACAGTCATACCTGTTACGACACCGACAGTTATTACACCTGTGGAAAGTACACCTGTTGGAGTG *F CAAACGGTTAGACCAGCCCAAACAGTTACACAAATTCGTATACAAACTACTGCACAACCTGCTAATGCGGCAGCAAATA *F CGAGAAAAGGCTTGTCTCTCACGCGAGAACAAATGTTGGAAGCACAAGAAATGTTTAGAACAGCTAATAAAGTAACTCG *F ACCAGAGAAAGCTCTTATTCTAGGTTTCATGGCTGGTTCAAGAGATAATCCTTGTCCAAAGTTGGGTAATATAGTCACA *F GTAATGCTCTCAGAAAATATAGAAGAAGTGACTCAACCGGATGGTACAACAGTTCCCATGTTGGTTGAGACACATTTCC *F AAATGAATTATACAAATGGCGAATGGAAGAGGATAAAGAAAAATCGACGAATTATTACAGAAGAATCAACGTCCACTAC *F GACTCCTACTCCCAGTGTGACGGCAACAGCTTCCAATTGAAAAAAAGATAAAAGAAATTTTTATCGAATTGCAATTATA *F TAGGTCAATAATTCTGTCATTTTTTGGATGACGGTGTTTTAAAAAACTCCTGTTCTATAGATAGCAAAAGAATTTGAGTG *F Indicates that CG5874 needs additional n and C-terminal sequence, encoded in *F genome; vertebrate matches agree. *F \***********A. mellifera Contig974 *F TTGGATACTCTTTTCATATTCTCGTGCTCACGTGCGTTATGCAAGAATTCATGCTTCTCCAAATTCTGATTGGTTTTTC *F TTTCTTGGCAACGGCAATTCCGAAGCCGGAAAATGATCACAAGCCGCGAGTTATTAATAAGGAACCAAACAGTGAAGAA *F CATTATGTCAATTCTCAACATAATCCTGCCTATGATCATGAAGTTTTTTTAGGTGAAGAAGCAAAAACTTTTGATCAGC *F TTACTCCTGAAGAAAGTACAAGAAGATTAGGAATAATAGTTGATAAAATAGATAAAGATAATGATGGTTATGTTACTGG *F AGAAGAACTTAAAGATTGGATATTATATTCTCAACGGCGTTACATACGGAACAATATTGAACATCAATGGAAATCTCAT *F AATCCTGAAGAAAAAGAGAAGCTTCCATGGACAGAATACTTAGCAATGGTTTATGGAGATATGGATGAACAGGAAGCAG *F AAAATCACGAAAAATCTAAAGATAATACTTTTTCGTATGCTGCTATGCTTAAAAAAGATCGCAGACGTTGGACAGCTGC *F AGATTTAGATGGTGATGATGCTCTTACAAAAGAAGAGTTTGCTGCTTTCCTTCATGTAGAGGAAGCTGATCATACAAAA *F GATATTGTAGTATTAGAAACCATGGAAGATATTGATAAAGATGGTGATGGAAAAATATCTCTTTCAGAATATATTGGTG *F ATGTATATGA *F This and several Drosophila ESTs suggest there are problems with the scf gene *F annotation. *F \***********A. mellifera BB260012B10B5.F *F GGGTTCACTGGTGGGCCTGGGTGGTGCTAAATCAGGTGGTAATACCCCGATGAACCCATCGCTACAACAGCGGATCAAC *F TTCCTCCAAAGTCATCTGAGCCAAGCACCAATGCCTTCCGTTGCTACCAAGAGGCGGCAACTGCCGTCTATAGAAGAGG *F CTTGGAACTTACCCATTAGTGCTGAGATGTCTAGTAGACAGCAACAACAGCAACAAACACCCACTGGTCCGGGTTATAA *F ATATGGTTCCACTCCTTCTGGACCACCACCTCCTTATCCTCAAGGACAAGGGCAGAATCTAAATACAAAAAGATTTAAG *F CCGGGAGAAGAACCAATTTCTCCAGGTTCACAACAGAGACCACCACCATTTTATCTCACGTCTCAACAACTGCAGATGT *F TACAGTTTCTTCAACAAAATCATGGAAGTTTAACGCAACAGCAGCAAGGTTTGCTTGCACAATTACAACAACAATACAG *F ATGTATGCAACAACATCAACAACAAATTAGATTACAACAGCAACAAGCTGCTCAAAGAGGTTTAAGGCCAGGACAACCT *F GGTTATCCTACAGGTTACAATCATTCACAACTAGGACAACCTGGCGTGATCAAGAATTACGGGATACCTCAGCAACCGT *F TGCAACAAGGTGGAACTGTTGCTTTACAAACAGGATTCTCAGATTCTAATGTCGGTTATAACACGGCAGCAACTGGGAA *F CAGTCAAAC *F This, mammal matches, and cDNA HL02950.5prime all seem to suggest there is a *F region missing from CG5640 *F \**********A. mellifera BB260013A20E9.F *F GAAACCTAGTGCTATGATAGTTTTTTCATTTATACTTTTATCATATTTTCTAGTAACTGGAGGTATAATATATGATGTA *F ATTGTGGAACCACCTAGTGTAGGCTCAACAACAGATGAACATGGCCATACAAGACCTGTAGCATTTATGCCGTATCGAG *F TAAATGGGCAATATATTATGGAAGGATTGGCATCTAGTTTCCTTTTTACATTAGGTGGAATTGGTTTTATAGTATTAGA *F TCAAACACATAATCCATCAACACCTAAGCTTAATAGAATTCTTTTAATATGTGTTGGATTTATTAGTGTTATTGTCTCA *F TTTATTACCTGTTGGGTTTTTATGAGAATGAAACTACCGTAAGATTTATATATATATATATATATTATATATTGTAATT *F TATAAAAGAACAAATAACACATTAATTAATTTATAAATACATTTTTTATATTTCTTTATACATTTAAATGAGACTTATT *F TACATACTTTTTTGTAATATACATATATAATATAAATACATAAGAAAAATAAAAAAAAGACTAGATTTTAAAAAAAAAA *F AAAAAAAAAAAAAAAAAAAAGCAAC *F This and vertebrate matches and bombyx mori EST indicate that there is an *F in-frame open reading intron in the annotation for CG9662 that should not be *F there, that is, it should be coding. *F \**********A. mellifera BB260014B10A5.F *F AGCTTGCGGCCGCGTTGCTTTTTTTTTTTTTTTTTTAATCGTCTCAATACGAAAGACCACGCGGATGTGTCGTTAGTGT *F GCACGTGTTCTTTACGCTCGCGCAGTGTTTCGTAAAGAGAAGAGGCCAAGATACATACGTACGCGGAACTGCAGCTGAA *F AGGGAGGAAAAGAGAAGAAACCCAACGAAGAAGAAAAGGAAGAAGTAGAAGATGAGCAAGACGTGCGCCCGCTGCGAGA *F AAACGGTCTACCCGATCGAGGAGCTCAAGTGCCTCGACAAGATATGGCACAAACAGTGCTTCAAGTGTCAGGGCTGCGG *F CATGATCCTGAACATGCGGACGTACAAGGGTTTCAATAAACAGCCATACTGCGAGGCGCACATACCAAAGGTGAAAGCC *F ACCACAATGGCCGAGACGCCGGAACTGAAACGCATCGCGGAGAACACGAAGATTCAGAGCAACGTGAAATACCACGCCG *F AATTCGAGAAGGCGAAAGGCAAGTTCACCCAGGTTGCGGACGATCCAGAGACACTGAGAATCAAGCAGAACAGCAAAAT *F TATCTCGAACGTTGCCTATCACGGTGAACTTCAAAAGAAGGCCATCATGGAGCAGAAAAGGACGATGACTGGTGAAAAT *F GGCGAACAGATCGTGACGAATCCACCGACCAGAAAGATTGGTTCTGTAGC *F This and vertebrate and C. elegans matches, and several Drosophila ESTs, show *F that the N-terminus needs revision, including a 5' exon about 16kb upstream in *F genome; plus current annotation is twice as big as the other matches. *F \**********A. mellifera BB260018B20E5.F *F TAAAAATGCCAAGGGGAAAATGGTATTGTTCTAATTGCCACAGTAAACAACCAAAGAAGAGAAATAGTAGTCGAAGGAG *F TCATACCAAAGGGGGAGGCACCAGAGAAAGTGAAAGTTCTGATCATCCACCAGCTAGTCCAACGCCGTCAACGGCATCG *F AACACACACGTAGAGGACGTCAGTTCATCGGAACCAGCAACCCCAACTGCCTCACCACGGAAGGAGGGAAACAATAGGA *F CGCTCACGAAGAAACAACAACGAGAGTTGGCTCCTTGTAAGGTGCTACTCGAACAGTTGGAGCAACAGGACGAGGCCTG *F GCCGTTCCTCTTGCCGGTGAACACCAAACAGTTTCCTACCTACAAGAAAATTATTAAAACACCCATGGATCTCAGTACT *F ATTAAGAAGAAATTGCAGGATTCCGTGTACAAGTCTCGCGATGAGTTTTGCGCCGATGTCAGACAGATGTTCATCAACT *F GCGAGGTATTCAACGAGGACGACAGTCCCGTGGGCAAGGCCGGACATGGGATGCGCAGTTTCTTCGAAATGCGTTGGAC *F CGAGATTACTGGGCACCACCTCCACACCCGCAACGCATAGCTGAGGCTCGGTTCGCTCTCGCAACACCTGCAACAGTTT *F TGCCCACTTCTACTACTAGAGGGTAAAACCCTCGAGAG *F Ortholog is CG10897, is annotated as mRNA, but not translated \- Two *F Drosophila ESTs show it well; *F \**********A. mellifera BB260019A10H3.F *F AGATTCTCGGGAAAGCCTTGAAGTAGCGATACAATGTTTAGAAAGTGCTTATAATGTACAAGCATCAGATACTCCAACA *F AATTTTAACTTATATGAAGTTTATAAGTCTTCCGTAGAAAATGCAAAACCTTATTTAGCTCCAGAAGCTACTCCAGAAG *F CAAAAGCTGAAGCTGAAAGATTAAAAAATGAAGGAAATACTCTCATGAAGGCTGAAAAACATCATGAAGCTCTTGCCAA *F TTATACAAAAGCAATTCAATTAGATGGTCGTAATGCTGTGTATTATTGTAACCGTGCTGCAGCATATAGTAAAATTGGC *F AATTATCAACAAGCAATTAATGATTGTCATACTGCATTGTCCATTGATCCCTCATACAGTAAAGCATATGGACGTTTAG *F GTTTAGCATATTCCAGCTTGCAAAGACATAAAGAGGCTAAAGAAAGCTATCAAAAAGCTTTAGAAATGGAACCTGACAA *F TGAAAGTTATAAAAATAATTTACAAGTAGCAGAAGAAAAATTAGCTCAGCCAAGCATGAGTAATATGGGATTAGGGGGA *F AGTGCATTACCAGGCATGGATCTTAGTTCACTCTTGAGTAATCCTGCTCTTATGAACATGGCTCGTCAAATGTTATCCA *F ATCCAGCTCTACAAAATATGGTGAGCAATTTTATGAGTGGACAAGTTGAACAGGGAGGACATATGGATGCTCTTATAGA *F AGCTGGTCAACATTTTGCACGA *F Human match is much better than Drosophila CG5094; but this may be because *F Drosophila has several large insertions that might be unspliced introns \- *F also not present in B. mori ESTs! *F \**********A. mellifera BB260019A20B12.F *F GTTGCTTTTTTTTTTTTTTTGTGAACCTGTGAGAATAGCGTTGCTTTCTCATTCGTGCCAAAGAGAATTCTGCCTGTCT *F TGTGAGCTAGGATTCCTGTTTCACATGTTGGATACATCTCGAGGATTGCCGTGTCAAGCTGCTAATTTTCTTCGAGCTT *F TTAGAACAGTACCTGAAGCGGCAGCTTTGGGACTTATACTCAGTGATCTCCATCCGGAGGCGAAAAGGAAAACAAATTT *F GGTACGATTAATACAGAGTTGGAACAGATTTATATTGCACCAGATTCATTATGAAGTTTTGGAAACAAGAAAACGACAG *F AAAGAGGAAGAAGAAGCTGCTCGATTAAAATCAGGACCAAAATGTCCACCGT *F Together with a new Drosophila EST shows there is an exon missing from CG8232 *F \**********A. mellifera BB260019A20D5.F *F AAAAAAGGAATTGAACTTCTTCGTATTCAATTTCCGATGTTCTGATTTAAAGATCAACTATAATGTTAACAGTGTCATT *F AATTTCATTTAAACGTGTGTGATCAAGTTAATTAAAAATAAAGTGTAAATAAACAACAAAATTCTTTGAAATATTTTAA *F GAGAGTACGAATGTTTTATTCGCTTTGATAACGCTACATTGCTTTGTCGTTTTTAACCTAAATCGAGATGGCTGATTCA *F GAACAAGATTTCGGAGATCGTGGAGATAATGACAATTTAAAAACTGATAAATTATTTATCTTAAAGAAATGGAATGCTG *F TAGCTATGTGGAGTTGGGATGTGGAATGTGACACTTGTGCAATTTGTCGAGTTCAAGTAATGGATGCATGTCTTCGATG *F TCAAGCGGAGAGCAAAAAAGATGATAGCCGACAAGACTGTGTCGTCGTCTGGGGAGAATGCAATCATTCATTTCATTAT *F TGTTGCATGTCACTTTGGGTGCAACAGAATAATCGTTGTCCATTATGCCAGCAAGAATGGTCCATTCAACGAATGGGAA *F AATAACTAAATCAATCAAGCGAAACTTCAATACTTATTTTGTTTCCTTTTTGTTTCGTTATTTTCTGCTTATTTTTCCT *F TCTTTCATTTCTTTCTCCCTTCTCTCTCACATATACACACATATGCACACGCACATACACATACACTCTCTCATTCACT *F CACTAAGTGG *F NEW GENE \- There is a Drosophila testis EST that has excellent match, but *F gene is unannotated. *F \***********A. mellifera BB260021A20F4.F *F GCGTTGCTTTTTTGTCTGCTCTTGATAAGATGGTTTCAGATAATATACAAGATAGAATGCGAGATTCAGTAAAACCACA *F ACAAGTAGATATTTCAGTTCCTTTACATGTAAAAAGTACTAAAAAAACATATGAACAATTGCAAGAAAGACCTTCTGAT *F AATAGTACAGTTGATTTTGTACTTATGTTGAGAAAAGGTAACAAGCAACAATATAAAAATTTAGCAGTTCCAGTATCAT *F CAGAATTAGCAATGAATCTTCGAAACAGAGAACAAGAACAGAAAGAAGAAAAAGAACGAGTTAAAAGATTGACATTAAA *F TATTACAGAAAGACAAGAGGAAGAAGATTATCAAGAAACAATTAATCAGAGTACCAAGCCAGTAACGGTAAACTTGAAT *F AGAGAACGGCGACAAAAATATAATCATCCCAAAGGTGCACCAGATGCCGATCTTATTTTTGGTCCTAAAAAAATACGGT *F AGATTTAATATTTTAATGTTTTTGGGACAAATTAATACTTTCCTATTAGAAAATCACAAGTGATCTAAGTTATGGACTA *F TTTGAAGTCCATATTTTTGTGTAGAATTTATAACAAATTAATAATTTTTATTTTTAATTTAGAATACATATCTACATAT *F ACATTATTTAAACGAATTTTCAACCATACTATATGATTTTTGGTGTAAAAATACATTTTACAATTATATT *F Comparisons with this and human homologs strongly suggest there is an extra *F intron near the C-terminus of this protein *F \**********A. mellifera BB260021B10D9.F *F GAATTTTCAACATGGTGCTAAGTGAAGTGAATAAATTTCTTCATGAATTAGAAAAAGCTGAATTAGAGGCGCCTGGTGG *F AGTTGCATCATCACAAACTTATGCTCAATTATTAGCTGTATATCTTTATCAAAACGATCTACCTGAACGAGAGAGgttt *F cgatctgtctttgtttgatcgaaaaatcgccgtcgcagATGCAATGCCAAATACTTGTGGAAGCGGATACCAACGGATC *F TGAAAAGCGGAAATGCAGAACTTGGTCAAATATGGATGGTAGGACAGCGTATGTGGCAAAGAGACTGGCCTGCAGTTCA *F TGTCGCCCTCAATGCAGAATGGAGTGAAGATGTTTCTGATATTATGGCTGCTTTGAAAGATAATGTTCGAGAAAGGGCA *F ATCACCTTAATATCAAAGGCTTATTCTTCACTAAGTTTAACCGTATTTGCGTCAATGACAGGCTTAACATTAGAGGAAG *F CGCGTCGTGTAGCAATTGAAAGGGGTTGGAACGTAGATGGAACGATGGTGCAACCTTGTAAGATTCAGAAAGAAGAGAG *F TAACCTCGTGAACGAGGTGTGTCTTACTGAGGATCAGCTGTACAAACTCACTCAATTCGTGTCTTTCTTGGAAAACTGA *F ACAAGCAATGAAAGTCATCAATGATGAAATTCACGCAACACAAAGACACGATCGACAGTACTTTAAACTTAGTTNTACC *F TCGTAATCATATTATATAAAGGAGGAAGTGTGCTTCAAAAGAGACACCTGTGTCCCTTNCCCTAGTATACACCCCGGAC *F TACCTACGCGTNCTGCTCATTCAATCGGAAGACTCGCGATGAAGCT *F Comparisons with human COP9 homolog show that the N-terminus is missing from *F the Drosophila homolog annotation, CG13383, and there is one available in the *F genomic sequences. *F \**********A. mellifera BB260021B20G3.F *F ATTTCCACAAAATTTATTAGCTTTGGCACCATTGCTACGTACATTGGATTTATCGGAAAATGAATTTGTTCATATTCCC *F GATAATATTGGTAATTTTACGTTATTAAAGCTATTGAATGTTAATCATAACAAATTGACAACTTTACCCGAAGCACTTG *F GAGCATTGACAAAATTAGAATGTTTAAATGCAAGTTCGAATCAAATAAAAACTATCCCATGGTCATTGTCAAAACTAAC *F ACGATTGAAACAAGTCAACTTATCTGATAATCGTATAACCGAATTTCCTCCTATGTTTTGTGATTTAAAATTTCTGGAT *F GTGTTAGATTTATCGAAGAATCGAATTACGACAATCCCTGATGCGGCTGGAGCGTTACATATAGTTGAACTTAACCTCA *F ATCAAAATCAGATATCAACTATATCTGAGAAATTGGCGGAATGTTCGCGCCTAAAAACATTAAGACTTGAAGAAAATTG *F TTTACAACTGAATGCAATACCTAGTAAAATTTTGAAAAATTCTAAAATTTCAGTCCTGTCTGTTGAAGGAAATTTATTT *F GAGATGAAACAATTTGCTAATCTTGATGGTTATGATAACTATATGGAAAGATATACCGCTGTAAAGAAAAAACTCTTTT *F AAGAGATATTTTAAATGAATATTTATTATTGAATCTATTATAGGTAATTATTATACATATATAATTATTTTATAATATT *F GAAAAAGATGCCGCATCGTGTTCGCGTAATTATACTCGATACCTGCGATAATATAATATAGAAATAAATTTTCAATTAA *F TTGATAAT *F This and vertebrate matches and cDNA GM01152 indicated that major reannotation *F of CG3040 is needed. *F \***********A. mellifera BB260024A20E12.F *F AGAAAAATCAGATGTTTGTTTAATTGGTCTACATGCTTGTGGAGATCTTAGTATACATGCATCAAAAATATTTCGAGAT *F ATGAAAATAGCACGTATTTTTATTTTAATTCCTTGTTGTTATCATAAGCTTTCAATATCAAAAAGGATAAGAATAAATA *F CATCAAGTGAAAAGCAATACTTTAATAATTTTCCTTTATCTAATTGTTTTAAAACTATTATTAATAATACTAATTTTGA *F TATTGGTACTTTTTTGAGGCAACCTTTTTTACGACTAGCATGTCAAGAACCAGTAGATAGATGGTATAACATGTCTATT *F GAAACACATAATAAACATTCTTTTTATGTTCTTGCAAGAGCTGTCCTTCAATTGTATGCAACTAAAAATGGATTTTCTC *F TTAAGAAATGTACTCAAAAAGGAACAAGAAAATCACAATGTTTAAATTTTGAAACATATATTAAAGATGCATTGACTAG *F GTACATTTTACAACCACAAGAAAAAGAAACATTCAAAAAACAAGATGTAGAATTTAATCTTGATACACATAAAAGAAAT *F ATAATAGAATTATGGAAAAATCATTGTGATAAATTTAAAATTGTAGAAATATATACTGGTTTACAACTGATGTTGCAAG *F CACCAGCAGAATCACTTGTTTTACAAGACAGATTATGTTGGATGGAAGAACAAGGTGGATTTCCTAATGATTGTCTGGA *F GTTTGTTGCTGAATTCCAG *F This and a B. mori EST and the human, Arabidopsis and C. elegans orthologs are *F indicate that the annotated N-terminus of CG8447 is incorrect, it should be *F another \+200aa. *F \***********A. mellifera BB270001B10B9.F *F ATCGCGATATAAAGGTGTTCCTGTAGAATATCTCGTGAGTTACATTAACCGAGTCATTCTGTCATCTGTTTCAAAGAAG *F AGAGAGGTCGGTGGCTCTGTGTATTAGTGTACAATCGCGCCATGGGGCATCAGTGTTGGTTCTTCACAAACGACGGGAC *F CTGAATGTGTGCATATTTCTCCTGGCTGACTGGCCTGCCTTCCACCGTTAAGCTGCATTCACAAAGGAAGCCGATTTTT *F GGGCAAGGACCGTATCTCGGTCGTTCTTGCCAAGAGTACGAATCGAACCGGTTAATGAGGACTATCTGGCTTCGATCGT *F CCAATCAGCTAGCAGCTAGACTGGACGAGCATAATCGGATGAAGTTGGGCCAAGCATGCCCTCTAAGAAGCAATATAAT *F CTCGTACATAATGACGAGTACGACACGAGGATACCACTGCACAGTGAAGAGGCATTCCACCGTGGAATTGTCTTCCATG *F CCAAGTTCATCGGCTCTATGGAGGTTCCTCGACCGACCAGCCGAGTGGAGATCGTGGCGGCGATGCGAAGAATCCGCTA *F CGAGTTCAAGGCCAAAGGGATCAAAAAGAAGAAAGTGACGCTGGAGGTATCCGTGGACGGGTTGAAAGTCACTCTTCGA *F AAGAAGAAGAAGAAGCAACAGCAGTGGATGGACGAGAATAA *F This EST and it's mammalian orthologs suggest major problems with annotion of *F CG17357 and CG3179 *F \***********A. mellifera BB270004A10C3.F *F GTTGCTTTTTTGCATCTTCATAGGATTGTGGATGTGCATTCCATTCGCTTGGACCAATCCAAAGGTGCAATCTCTCAAG *F TCTATGGAGGTGGATTGGATCGGTGAAGTGAAGCCTGGGGAATACTGGTCTTACGTGGATTATGGTCTTTTGTTGATAT *F TCGGTGGTATCCCTTGGCAAGTATATTTCCAACGTGTCCTGTCCTCGAAAACTGCTGGAAGAGCGCAAGTGTTGAGCTA *F CGTAGCCGCGATAGGGTGCATTATCATGGCCATACCACCTGTCCTGATCGGTGCACT *F This EST and vertebrate matches show problems with the C-terminus of CG7708 *F \***********A. mellifera BB270007B20H3.F *F GTTGCTTTTTTTTTTTTTTTTTTTATTAAAACATATAATTCATAGTAATTAATCAATAGATTCATATTGCAGCGTGAAA *F CATGTCGAATAAATTTGAAGCGTTTGCAAGTGTGGAGCAGTTTTGGAGTCTTTACAGTCATTTAGTCCGGCCATCAGAA *F TTAACAACATCTACAGATTTTCATCTTTTCAAAGTTGGCATAAAACCAATGTGGGAAGATGAGGCAAATCAAAAAGGTG *F GTAAATGGATAGTACGATTAAGAAAAGGTTTAGTTTCTAGATGTTGGGAAAATCTTATATTAGCTATGTTAGGAGAACA *F ATTTATGGTTGGAGAAGAGATATGTGGAGCTGTTGTATCTATAAGGTTTCAAGAGGATATAATATGTGTATGGAATAAG *F ACTGCATCTGATTATGCAACAACAGCACGTATTAGAGATACATTAAGGAGAGTTTTACATCTTCCAGCAAGTGCCTCAA *F TGGAATACAAAACTCATAATGAAAGTTTAAAGAATGTTCATCGGCTCTAAAATCTTGTGATGTCAACTCAAAGATTTGA *F ATTCTTTATGGATTTTCAGCCAATTGATACTTGTT *F This unspliced EST and it's vertebrate matches indicate there is a problem *F with the C-terminus of CG10716; the appropriate sequence is available in the *F genome sequences. *F \**********A. mellifera BB270021A20F1.F *F ATTATTGTGAATATTGTGATAGATCGTTTAAGGATGATCCGGAAGCCAGAAAAAAACATCTTTCAAGTTTGCAACATGC *F GAAAAATCGTGCAGATCATTATAATATGTTCAAAGATCCAGAAATTATTTTAAGGGAAGAATCTACAAAGATACCATGT *F AAATGGTATTTAACTAATGGTGAATGTGCATTTGGCCTTGGTTGCAGATATTCCCATTATACTCCTCCTATGATATGGG *F AACTTCAACGTCTTGTTGCTATGAAAAATCAATCAAAGTTGAATATAAATCTCGAAAATGGCTGGCCAAATCCTGACGA *F TATAATTAAAGAATATTTTGAGAATAATACGAGCACAAGCACTACAGATGATTTTACGTATCCAAATTGGCACAGACCA *F TCGGAGCTACATGATTATTCTATGCTATCACCATCGTTATGGCCTATTACGCCTGAAAGTTTAGCAAATACTACAAGAT *F TCGAAGAATGGGGTTAAAAATGTAAGGAATATAATGTTCCTTGTTAAATTAAATGAAAATACATATATTTAAGACTCGT *F CCAAAAGAGTAAATAATATATGATATATAAACAATTTAAATATATAGTTTTTCAAAAAACTGAATATTTTGTTAATAAA *F TGAACATATAAAATTGGGAAATGTGTTCACTTTCTTAAATAGGAAATATTATTGTTAATATTATATTGTAATAATATTA *F TT *F Bee translation Y C E Y C D R S F K D D *F P E A R K K H L S S L Q H A K N R A D H Y N M F K D *F P E I I L R E E S T K I P C K W Y L T N G E C A F G *F L G C R Y S H Y T P P M I W E L Q R L V A M K N Q S *F K L N I N L E N G W P N P D D I I K E Y F E N N T S *F T S T T D D F T Y P N W H R P S E L H D Y S M L S P *F S L W P I T P E S L A N T T R F E E W G Z *F NEW GENE \- completely unannotated \-between CG5105 and CG5118 \- also *F mammalian matches *F AE003587 *F ATGGGTGGCAAAAGTTATTATTGCGACTACTGCTGTTGCTTTCTGAAAAACGATCTGAATGTGAGGAAATTGCACAATG *F GTGGTATTGCACACGCAATTGCAAAGAGCAACTATTTGAAGCGTTACGAGGgtaaagcttttgttgcacgatattcccg *F aaaaagctgaattttcaatatttgtagATCCCAAAAAGATTTTGACTGAAGAGCGGCAGAAAACTCCTTGCAAGCGATA *F CTTTGGCAGTTACTGCAAGTTTGAAACATATTGCAAGTTTACCCACTATAGTGGCGATAATCTACGGGAACTGGAGAAG *F TTGGgtgcgtgataaaacgcagatttaaaacgaaaaataacttcttcttgaaaactttcagTTCTCGCTAGAAAGAAGA *F GAAAATCCCGAAAGAAAACCAACAAATGCAAGAGATGGCCCTGGAAAACTCATCTGCGAAAGGGATTACCCCCTTCCTT *F GCAACCCATTAACCCGGAAAAACTCAAGCAAACCGACTTTGAACTCAGTTGGGGCTAAATATATTTACAGAATGCACAC *F TTTATGTCAAGTATTCAGTATGCTAATTACTTTCTCCATGACGCGCGCCACTTTCAGGTTG *F translation M G G K S Y Y C D Y C C C F L K N D L N *F V R K L H N G G I A H A I A K S N Y L K R Y E *F \---------------------------1---------------------------D P K K I L T E *F E R Q K T P C K R Y F G S Y C K F E T Y C K F T H Y *F S G D N L R E L E K L *F \-------------------------------1-------------------------V L A R K K R *F K S R K K T N K C K R W P W K T H L R K G L P P S L *F Q P I N P E K L K Q T D F E L S W G Z *F Nice small gene with two short introns *F Fly *F MGGKSYYCDYCCCFLKNDLNVRKLHNGGIAHAIAKSNYLKRYEDPKKILTEERQKTPCKRYFGSYCKFETYCKFTHYSG *F DNLRELEKLVLARKKRKSRKKTNKCKRWP------------------------------WKTHLRKGLPPSLQPINPEK *F LKQTDFELSWGZ *F Bee *F YCEYCDRSFKDDPEARKKHLSSLQHAKNRADHYNMFKDPEIILREESTKIPCKWYL-TNGECAFGLGCRYSHYTPPMIW *F ELQRLVAMKNQSKLNINLENGWPNPDDIIKEYFENNTSTSTTDDFTYPNWHRPSELHDYSMLSPSLWPITPESLANTTR *F FEEWGZ *F \**********A. mellifera BB270024A20E1.F *F AACAGGAAAGGCTTGACGCCGCTCTACTACAGCGTCATCTACAAAACCGATCCGATGCTGTGCGAAACGTTGCTCCACG *F ACCACGCGACGATAGGCGCCCAGGATTTGCAGGGATGGCAGGAAGTGCATCAGGCCTGCCGCAACAACCTGGTCCAACA *F CCTGGATCACTTGCTCTTTTACGGTGCCGACATGAACGCGCGTAACGCGTCCGGCAACACGCCGTTGCACGTATGCGCT *F GTGAACAACACGGACTCGTCGTGCATACGCCAGTTGCTGTTCAGAGGCGCGCAGAAGGACAGCCTGAATTACGCGAACC *F AGACTCCCTACCAGGTGGCGGTGATCGCTGGGAACATGGAGCTGGCCGAGGTCATTAAGAATTATCAGCCGGAAGAAGT *F TGTACCGTTTAAAGGGCCGCCACGCTACAACCCGAAACGGCGCTCGGTGGCGTTCGGAGGCACGTCGACGATGACCACT *F AGCTGCTCGGCCAGCAACTTGGGCACCCTGACCAGGATACCGTCCGCCGAACACCAACACGCCTCTGGCGGAACAGGAG *F GAGGAGGAGGAGGAGGGGGAGGTGGTAGCCTGACCAGAACGATCTCAGTGGAGCAGTACGCGGTGACGAGAGTACCGTC *F CGCCGAGCAATACGCGACCGGCAACCTGACCAGAGTACCGTCCACGGAACAATACGCCAGCCCTATCGCGACCGCGAC *F This EST and it's vertebrate matches suggest there is a major unannotated *F region upstream of CG8122 that should be added to it. *F \**********A. mellifera BB270025A20A3.F *F AGTTCTAGATCTTGCCACTGAAACTGCTACTGCTGTAAGAGAAACAAGTAGAAGTGCTCATCGTACGATACCAAAACGC *F GATAGACCTCCTCGTGTGGCAAGTGGTTCTGCTGGTCTATTACCACCCTATAATCGCCAACAAGCAGAGGGCCAAGAAT *F TTCTTTATATAATAAATGAACATAATTATTCAGAATTATTTGTGGCATATGAGTGTTTACGTAGTGGAACGGAGAATCT *F AAGAATTCTTGTTTCTAATGAAAGAGTTCGAGTGATTTCCGGAGGTACCAAAGGAGTTGTAACCGAAGTCAGTCTAGCG *F GACTTATTATATTGTCAACCAATGCATAAGCTAGAAAGTAATGGTGTTACTTTATACTATATTGAATTAATATCTAGAT *F CAGATTCAACGATAACCGTTAACATGGACGGTCCAGAACTTCTAAGAAGACCTAAAGTTCGATGTGACAATGAAGAAGT *F AGCCAAAAGAGTATCGCAGCAAATTAATTACGCTAAAGGAATGCACGAGGAACGTAGCTTGACTCTTTCTTCTTCGGAT *F AATATGTTAGATGATGTACAGTACTATAAGTAGTTACAAACAATCATATATGAAAATTTATTTTGTATTTGACAAAAGT *F TTGGAATCACTATGTTTTTACAAAAAATTTTTATGGGAAATTAATGCATTAAAATATTTTCATTTCAATGTTAATTCC *F This EST plus it's human match show that the C-terminus of CG11003 is *F incorrectly annotated. *F \**********A. mellifera BB270031A10B1.F *F GAAGACAGAAAGCTGTTCGTGGGAATGCTCAGCAAGCAACAAACAGAAGACGATGTCAGACAGTTGTTCACTGCCTTTG *F GCACAATAGAGGAGTGTACCATCCTCCGAGGACCTGACGGCAGTAGCAGAGGCTGTGCATTCGTAAAACTTTCATCGCA *F TCAAGAAGCGCTAGCGGCGATCAATACCTTACACGGTAGCCAAACTATGCCGGGTGCGTCATCCAGCTTAGTGGTAAAG *F TTCGCAGATACTGAGAAAGAGAGACAACTAAGACGCATGCAGCAAATGGCCGGGAACATGAGCCTCCTTAACCCTTTCA *F ACGTCTTCAATCAGTTCGGCGCTTACGGCGCTTACGCTCAGCAGCAAGCAGCCCTGATGGCTGCGGCAACGGCACAAGG *F GACGTATATCAATCCAATGGCGGCATTGGCACACGTTGGCGCTGGCCAACTGCCGCACGCGTTGAACGGCATGCCAAAC *F CCCGTCGTTCCACCGACTTCCGGTTTGCTCGTAGGTACCGGTACAGGGCAGCCTGTTAACGGGGCGATACCGTCGTTAC *F CCA *F This EST suggests that CG12478 connects to CG10046. *F \**********A. mellifera BB270032A10D3.F *F TCGTCCACTAACGCGGGAGGAGCGGGTGCACCCAGCGAAGACAATACGCAGATATTGGTGATGAACAATTACTTCGGTA *F TCGGCCTCGATGCCGATCTTTGTTTAGACTTTCACAACGCCAGGGAAGAAAATCCGAATAAATTTAAAAGCAGATTGCG *F TAACAAAGGGGTGTACGTAACCATGGGTTTGCGAAAAATGGTAAAACGGAAACCGTGCAAAGATTTGCACAAAGAGATA *F CGACTGGAAGTGGACGGGAGACTCGTCGAATTACCTCAAGTCGAAGGAATAATTATTCTAAACATTTTAAGTTGGGGTT *F CTGGGGCAAATCCTTGGGGACCAGACATCAAGGAAGACCACTTTCAAACACCGAATCACGGGGATGGGATGTGGGAAAG *F TTGGCGAAGTCACGGTGTTTGCATCTTTGGACAAATCCAATCTGGTCTCCGTACAGCGATGAGGATAGCACAGGGTGGA *F CATATAAAAATTCATTTGTACTCCGACATACCAGTGCAAGTAGACGGAGAACCATGGATCCAGAGTCCGGGGGATATCG *F TAGTTCTGAAATCGGCACTGACGGCCACCATGTTGAAGAGCATAAGATCAAGCGTCGGAATACCGAACCTTCGATTCCA *F CCCGCTAATGGGGTGGGGGGCAAGAGCTCGGACGAGTGTCGCGACGAAAGCTTGATGCCGCAGTTTCCGCGCTA *F This EST and its nematode and human matches show that there is a long *F C-terminus to CG5875; and there is a Drosophila EST. *F \**********A. mellifera BB270032B10E8.F *F TTCAATAACACCATCAACAGAAAACTCAATTAGTCCAGAACCTGAGATTAAACCATTGACAGACATTAATATTAATCTT *F CATGATATTAAACCAGGTATTAATCCACCTATAACAGTAATTGAAGAAAAAAATGGTATATCCGTAGTGCTTCATTTTG *F CTCGAGATAATCCAAGAAAAGATGTATTTGTTGTAGTGATTACAACAATGAGTAAGAATTTGAAGCCACTTACTAATTA *F CTTGTTTCAAGCAGTGGTGCCAAAAAGATGTAAATGTAGACTTCAGCCACCTTCTGGAACAGAATTGCCTGGTCATAAT *F CCATTTTTACCTCCATCTGCAATTACTCAAATTATGTTAATTGCAAATCCTACCAAGGAAACGGTATCGTTAAAATTTA *F TGTTAAGTTATACTATGGATGATGAAACTTTCACAGAAATGGGTGAAGTAGAAAAATTGCCTTTAGTTTAAAGTACTTA *F AAGTGTAATTATAATATAAATTAAATTCAAGAATTACAGACTCTAATAGCCAAAAGAAGAATATATTGTTTTTTAAGAT *F ATGAATTTTCAAAAGATATTGTTTCTTTTACTTTATTTCTCAGATTGTAATTACAGTTTTATCTTTATTATTATATTCT *F GAGATATATTAATGTTATGTAGATATTTATAAGTCATGTTGTGATTACATAACGAAAATATCAATAACAATCTATTTTA *F AGATCGA *F This EST and mammalian matches indicate the C-terminus of CG3002 is not *F annotated \- it is there in the genome. *F \------------------------------------------------------------------------------ *F -- # *U FBrf0136024 *a Robertson *b H.M. *t 2001.5.16 *T personal communication to FlyBase *u FlyBase error report on Wed Mar 16 12:13:50 2001. *F >From hughrobe@life.uiuc.edu Wed May 16 18:13:41 2001 *F Date: Wed, 16 May 2001 12:13:50 -0500 *F Subject: Re: annotations of Drosophila Or and Gr genes *F To: gm119@gen.cam.ac.uk, eleanor@ebi.ac.uk *F *F Dear Gillian and Eleanor, *F *F Attached are three WORD MAC TEXT files, with my suggestions for annotations *F improvements for the Or family. There are FASTA files of the cDNAs and *F proteins, and a text file of comments on each gene (let me know if you have *F problems opening them and I will paste them into the email body). Will *F updating these have to wait for Release 3, or would you we able to submit *F updated files to GenBank in the interim? *F *F Thanks for helping with this. *F *F Hugh *F *F *F \******************************************************************* *F Hugh M. Robertson *F Professor *F Department of Entomology *F University of Illinois at Urbana-Champaign *F 320 Morrill Hall, MC118 *F 505 S. Goodwin *F Urbana, IL 61801 *F Phone 217-333-0489 *F FAX 217-244-3499 *F Email hughrobe@life.uiuc.edu *F www.life.uiuc.edu/robertson/lab.html *F *F ------------------------------------------------------------------------------- *F *F Odorant receptor annotation notes: *F *F Comments on annotation of the Or genes in Drosophila, for Gillian *F Millburn and Eleanor Whitfield 5/15/01 *F *F I make these suggestions largely based on my bioinformatic analyses, *F which include careful alignments, phylogenetic analysis, and analysis *F of intron evolution. Where there is available cDNA information I *F mention it. There are no ESTs and no comparable genes available from *F other species. If a gene is not mentioned here I accept it as is. All *F of these are differences with the results of BLASTP searches of the *F NCBI 'Drosophila Genome' database - most of the annotations appear to *F be from Release 1 so some may already have been updated, but are not *F available there. They have been accepted by John Carlson's group at *F Yale (specifically Coral Warr) and by Leslie Vosshall at Rockefeller *F (previously Columbia). I've tried to get responses from the other *F group involved in the original descriptions, Andrew Chess at MIT, but *F without success. *F *F Or1a - CG17885 - This annotation needs an additional terminal intron *F and exon to encode an alignable C-terminus, which is the most conserved *F region of the protein involved in TM7. *F *F Or13a - CG12697- This annotation needs major revision at both ends. *F One intron uses a GC 5' splice donor, but hopefully that is acceptable, *F as it provides good alignable amino acid sequence and its location and *F phase is conserved in other receptors (GeneFinder predicts nearby phase *F 2 and 1 intron splice sites, but they can't sensibly be employed). *F Such a GC donor is already used in the sole intron of gene Or2a. *F *F Or19a - CG18859 - The C-terminus of this annotation needs revision to *F encode an alignable TM7 region. Also, there is a perfect copy of this *F sequence about 50kbp further along, presumably as part of a large *F duplication - I've tried to find out from Sima Misra and Sue Celniker *F if this is real, but no response yet. *F *F Or22c - CG15377 - This annotation needs a different C-terminus. *F *F Or24a - CG11767 - This annotation has an intron that I return to the *F coding sequence, because it has an unpredicted 3' splice acceptor, *F would be a unique intron placement/phase in the Or family, is in-frame *F and open, and encodes a section needed for decent alignment with its *F closest relatives. *F *F Or30a - CG13106 - Need to add two exons to this prediction to yield an *F alignable 7TM receptor. *F *F Or35a - CG17868 - I add an exon for needed amino acids for alignment *F with relatives. *F *F Or42a - CG17250 - This needs a final intron and exon to encode the *F conserved TM7 region. *F *F Or42b - CG12754 - This needs to start a little earlier to encode an *F alignable N-terminus and complete TM1 region. *F *F Or43b - CG17853 - There is an open in-frame but well predicted intron *F in a conserved location near the C-terminus that needs to be removed. *F *F Or46a - CG17849 - The innards of this annotation need rearrangement to *F align a reasonable match to its closest relative and neighbor, 46b. *F *F Or46b - CG17848 - There is a final intron/exon missing from the *F annotation. *F *F *F Or49a - CG13158 - Two sections need restoring to encode a full-length *F 7TM receptor alignable with its closest relatives. *F *F Or56a - CG12501 - This protein needs an N-terminus, and the one below *F works in terms of providing the first couple of TM domains, albeit *F without clear sequence similarity to other family members. There is *F also an added exon near the C-terminus needed for alignment. *F *F Or63a - CG9969 - Just one more in-frame open intron needed near the *F C-terminus. It is in a well-conserved position within TM7 *F *F Or65a - not annotated *F *F Or65b - not annotated - this uses a 5' donor GC site, which is in *F precisely the same location as a good GT intron in 65c, and others. *F See also comments for Or13a *F *F Or65c - not annotated *F *F Or67a - CG12526 - This one needs help at both ends. *F *F Or67b - CG14176 - This needs two more exons currently in introns, plus *F a changed boundary. *F *F Or67c - CG14156 - This needs changes at the C-terminus and internally, *F and could perhaps use a slightly longer N-terminus. *F *F Or67d - CG14157 - This needs a lot more on the insides. *F *F Or69b - CG17902 - The annotation is also tricky because the current *F C-terminus is very unlikely, indeed we propose that this gene is *F alternatively spliced to the final two exons of 69a below. Only the *F first long exon is shown in the FASTA files. *F *F Or69a - not annotated. *F *F Or71a - CG17871 - This one needs a final intron and exon, and a small *F adjustment to the current intron boundary for best alignment with 94a/b *F *F Or82a - not annotated *F *F Or83a - CG10612 - This needs a final intron and exon to encode the *F conserved TM7 region. *F *F Or83b - CG10609 - The second intron, which is in-frame and open and has *F completely unpredicted boundaries, is not spliced, according to RT-PCR *F data from Coral Warr, and a cDNA from Leslie Vosshall *F *F Or83c - CG15581 - Needs an N-terminus. *F *F Or85b - CG11735 - Needs the final conserved intron/exon for an *F alignable TM7 region. *F *F Or85c - CG17911 - Like 85b this needs the final conserved intron/exon, *F plus a different boundary on the penultimate intron. *F *F Or85e - CG9700 - the current annotation is probably as good as can be *F done, but it lacks the conserved C-terminus, which is present in a cDNA *F from Vosshall et al (AF127922). The strange thing is that the *F C-terminus in this cDNA is not present anywhere in the genomic *F sequence! *F *F Or88a - CG14360 - a minor difference in starting codon, I would include *F MKPTEIKKPYR. *F *F Or92a - CG17916 - This annotation needs a C-terminus *F *F Or98a - CG5540 - This needs a slightly later 5' donor splice site on *F the first intron to encode well aligned amino acids. *F *F Or98b - CG1867 - This one has an in-frame open poorly predicted intron *F in the middle (exactly the same position as in its closest relative *F 47a) and needs a different C-terminus to align well with 47a and *F others. *F *F *F ------------------------------------------------------------------------------- *F *F Odorant receptor cDNAs FASTA: *F *F >Or1a *F ATGTCAAAGCTAATCGAGGTGTTTCTGGGTAATCTGTGGACGCAGCGTTTTACCTTCGCCCGAATGGGTTTGGATTTGC *F AGCCCGATAAAAAGGGCAATGTTTTGCGATCTCCGCTTCTTTATTGTATTATGTGTCTGACAACAAGCTTTGAGCTCTG *F CACCGTGTGCGCCTTTATGGTCCAAAATCGCAACCAAATCGTGCTTTGTTCCGAGGCCCTGATGCACGGACTACAGATG *F GTCTCCTCGCTACTGAAGATGGCTATATTCTTGGCCAAATCTCACGACCTGGTGGACCTAATTCAACAGATTCAGTCGC *F CTTTTACAGAGGAGGATCTTGTAGGTACAGAGTGGAGATCCCAAAATCAAAGGGGACAACTAATGGCTGCCATTTACTT *F TATGATGTGTGCCGGTACGAGTGTGTCATTTCTGTTGATGCCAGTGGCTTTGACCATGCTTAAGTACCATTCCACTGGG *F GAATTCGCGCCTGTCAGCTCGTTCCGGGTTCTGCTTCCATACGATGTGACACAACCGCATGTTTATGCCATGGACTGCT *F GCTTGATGGTATTTGTGTTAAGTTTTTTTTGCTGCTCCACCACCGGAGTGGATACCTTATATGGATGGTGTGCTTTAGG *F CGTGAGTTTACAATACCGTCGCCTCGGTCAACAACTTAAAAGGATACCCTCCTGTTTCAATCCATCTCGGTCTGACTTT *F GGATTAAGTGGGATTTTTGTGGAGCATGCTCGTCTGCTTAAAATAGTCCAACATTTTAATTATAGTTTTATGGAGATCG *F CATTTGTGGAGGTTGTTATAATCTGTGGACTCTATTGCTCAGTAATTTGTCAGTATATAATGCCACACACCAACCAAAA *F CTTCGCCTTTCTGGGTTTCTTTTCATTGGTAGTTACCACACAGCTGTGCATCTATCTTTTCGGTGCCGAACAGGTCCGT *F TTGGAGGCTGAGCGATTTTCCCGGCTGCTATACGAAGTAATTCCTTGGCAAAACCTTCCTCCTAAACACCGGAAACTTT *F TCCTTTTTCCAATTGAGCGCGCCCAACGAGAAACTGTTCTCGGTGCTTATTTCTTCGAACTAGGCAGACCTCTTCTTGT *F TTGGATATTTCGCACAGCAGGCTCTTTTACAACTTTGATGAACGCTCTCTACGCAAAATACGAAACGCATTAA *F *F >Or13a *F ATGTTCTATTCGTATCCCTACAAAGCACTTAGCTTTCCCATTCAGTGCGTTTGGCTGAAACTGAATGGTTCTTGGCCAT *F TAACCGAATCATCGAGGCCATGGAGGAGCCAATCCTTATTGGCCACCGCCTACATCGTGTGGGCGTGGTACGTCATTGC *F ATCTGTGGGCATAACAATCAGCTATCAGACGGCCTTTTTGCTGAACAACCTTTCGGACATTATTATCACCACGGAAAAT *F TGTTGCACCACCTTTATGGGTGTCCTGAACTTTGTCCGACTCATCCATCTTCGCCTCAATCAGAGGAAATTCCGCCAGC *F TTATTGAGAACTTTTCCTACGAAATTTGGATACCTAATTCTTCCAAAAACAATGTTGCCGCCGAGTGTCGCAGACGCAT *F GGTTACCTTCAGCATAATGACATCCTTGCTAGCGTGCCTGATCATAATGTATTGTGTCCTGCCGCTGGTGGAGATCTTC *F TTTGGACCCGCCTTCGATGCACAGAACAAGCCGTTTCCCTACAAGATGATCTTTCCGTACGATGCCCAGAGCAGTTGGA *F TCCGATATGTGATGACCTACATCTTCACCTCCTACGCGGGAATCTGTGTGGTCACCACCTTGTTTGCAGAGGACACCAT *F TCTTGGCTTCTTCATAACCTACACTTGTGGCCAATTTCATTTGCTACACCAACGAATCGCAGGTTTATTTGCGGGTTCC *F AATGCGGAATTGGCCGAGAGCATTCAGCTGGAGCGACTCAAACGTATTGTGGAAAAACACAACAATATTATCAGTTTTG *F CTAAGCGCCTCGAGGACTTTTTCAACCCAATTCTACTGGCCAATCTAATGATTTCCTCCGTACTTATTTGCATGGTTGG *F CTTTCAAATAGTTACTGGAAAAAATATGTTCATTGGAGACTATGTGAAGTTCATAATTTACATATCCTCGGCCCTTTCA *F CAATTATACGTTCTTTGCGAGAATGGCGACGCATTGATAAAACAGTCTACCTTAACAGCGCAAATTCTGTATGAATGCC *F AGTGGGAAGGATCCGATCGCATTGAGATACAATCGTTCACGCCAACAACCAAGCGAATCCGAAACCAGATTTGGTTTAT *F GATCCTCTGCAGTCAACAGCCTGTAAGGATTACGGCTTTCAAGTTCTCAACGTTGTCCTTGCAGAGTTTTACGGCCATT *F CTAAGTACCTCGATAAGTTACTTTACATTATTGCGGTCAGTTTACTTCGACGACGAAAAGAAACTAGATTAA *F *F >Or19a *F ATGGACATATCGAAGGTGGATTCAACGAGGGCTCTGGTTAACCACTGGCGCATCTTCAGGATTATGGGAATCCATCCGC *F CGGGCAAGAGGACCTTCTGGGGTCGCCACTACACGGCGTACTCCATGGTGTGGAACGTAACCTTCCACATCTGCATCTG *F GGTGTCCTTTTCGGTCAATCTCCTGCAGTCCAATTCGCTGGAGACTTTCTGCGAGAGCCTCTGCGTGACCATGCCGCAC *F ACGCTCTACATGCTTAAGCTGATCAATGTCCGTCGGATGCGCGGCCAGATGATCAGCAGCCACTGGTTGCTCCGTCTCT *F TGGACAAGCGGCTTGGCTGCGACGACGAACGCCAGATCATTATGGCCGGCATCGAGCGGGCCGAGTTCATATTCCGCAC *F CATTTTTCGCGGCCTTGCGTGCACCGTCGTCCTTGGCATCATCTACATATCCGCGTCCAGCGAGCCCACGCTGATGTAC *F CCCACCTGGATTCCCTGGAACTGGAGGGACAGCACCTCCGCCTACCTGGCCACCGCCATGCTGCACACGACCGCCCTCA *F TGGCGAATGCGACACTTGTCCTCAATCTGAGCTCCTATCCGGGCACCTACCTCATCCTGGTCAGTGTCCACACCAAGGC *F GCTCGCCCTGCGGGTCTCCAAATTGGGATATGGCGCGCCACTACCGGCGGTTCGGATGCAGGCCATTCTGGTTGGTTAC *F ATCCACGACCACCAGATCATTTTGCGCCTCTTCAAGTCACTGGAGAGATCCCTTTCGATGACCTGCTTTCTGCAGTTCT *F TCAGCACGGCGTGTGCGCAGTGCACAATCTGCTACTTTCTACTCTTCGGGAACGTCGGGATCATGAGGTTCATGAATAT *F GTTGTTCCTGCTGGTGATCCTCACCACGGAGACCCTTCTTCTCTGCTACACGGCGGAGCTACCTTGCAAGGAAGGGGAG *F AGCCTCCTGACCGCTGTCTACAGCTGCAACTGGCTGTCCCAGTCGGTAAACTTTCGGAGACTCCTGCTCCTGATGCTCG *F CACGCTGCCAGATTCCAATGATCCTGGTCTCCGGCGTAATTGTGCCCATCAGCATGAAGACCTTCACGGTGATGATTAA *F GGGAGCGTACACCATGCTTACTCTGCTGAATGAAATTCGTAAAACGTCCCTTGAATAG *F *F >Or22c *F ATGACTGACAGCGGGCAGCCTGCCATTGCCGACCACTTTTATCGGATTCCCCGCATCTCCGGCCTCATTGTCGGCCTCT *F GGCCGCAAAGGATAAGGGGCGGGGGCGGTCGTCCTTGGCACGCCCATCTGCTCTTCGTGTTCGCCTTCGCCATGGTGGT *F GGTGGGTGCGGTGGGCGAGGTGTCGTACGGCTGTGTCCACCTGGACAACCTGGTGGTGGCGCTGGAGGCCTTCTGCCCC *F GGAACCACCAAGGCGGTCTGCGTTTTGAAGCTGTGGGTCTTCTTCCGCTCCAATCGCCGGTGGGCGGAGTTGGTCCAGC *F GCCTGCGGGCTATTTTGTGGGAATCGCGGCGGCAGGAGGCCCAGAGGATGCTGGTCGGACTGGCCACCACGGCCAACAG *F GCTCAGCCTGTTGTTGCTCAGCTCTGGCACGGCGACAAATGCCGCCTTCACCTTGCAACCGCTGATTATGGGTCTCTAC *F CGCTGGATTGTGCAGCTGCCAGGTCAAACCGAGCTGCCCTTTAATATCATACTGCCCTCGTTTGCCGTGCAGCCAGGAG *F TCTTTCCGCTCACCTACGTGCTGCTGACCGCTTCCGGTGCCTGCACCGTTTTCGCCTTCAGCTTCGTGGACGGATTCTT *F CATTTGCTCGTGCCTCTACATCTGCGGCGCTTTCCGGCTGGTGCAGCAGGACATTCGCAGGATATTTGCCGATTTGCAT *F GGCGACTCAGTGGATGTGTTCACCGAGGAGATGAACGCGGAGGTGCGGCACAGACTGGCCCAAGTTGTCGAGCGGCACA *F ATGCGATTATCGATTTCTGCACGGACCTAACACGCCAGTTCACCGTTATCGTTTTAATGCATTTCCTGTCCGCCGCCTT *F CGTCCTCTGCTCGACCATCCTGGACATCATGTTGAACACGTCGTCGTTGAGCGGCTTAACCTACATCTGCTATATCATC *F GCGGCCCTAACGCAGCTATTCCTCTACTGCTTCGGAGGCAATCACGTCAGCGAGAGTAGTGCGGCTGTGGCGGACGTGC *F TGTACGACATGGAGTGGTACAAATGCGATGCGAGGACTAGGAAAGTGATTTTAATGATATTGCGCCGTTCGCAGCGGGC *F AAAAACAATTGCGGTGCCGTTTTTTACGCCCTCACTGCCAGCACTCCGATCTATACTCAGCACAGCCGGCTCATATATC *F ACGCTGCTAAAGACGTTCCTGTAA *F *F >Or24a *F ATGGAGCGCCATTATTTCATGGTGCCAAAGTTTGCATTATCGCTGATTGGTTTTTATCCCGAACAGAAGCGAACGGTTT *F TGGTGAAACTTTGGAGTTTCTTCAACTTTTTCATCCTCACCTACGGCTGTTATGCAGAGGCTTACTATGGCATACACTA *F TATACCGATTAACATAGCCACTGCATTGGATGCCCTTTGTCCTGTGGCCTCCAGCATTTTGTCGCTGGTGAAAATGGTC *F GCCATTTGGTGGTATCAAGATGAATTAAGGAGTTTGATAGAGCGGAGGTTCTATACACTGGCAACGCAACTAACATTCC *F TGCTACTATGCTGTGGATTTTGCACCAGTACTTCCTATTCCGTCAGACATTTGATTGATAATATCCTGAGACGCACCCA *F TGGCAAGGACTGGATCTACGAGACTCCGTTCAAGATGATGTTCCCCGATCTTCTCCTGCGTTTGCCACTCTATCCCATC *F ACCTATATACTCGTGCATTGGCATGGCTACATTACTGTGGTTTGTTTTGTCGGCGCGGATGGTTTCTTCCTGGGGTTCT *F GTTTGTACTTCACTGTTTTGCTGCTCTGTCTGCAGGACGATGTTTGTGATTTACTAGAGGTTGAAAACATCGAGAAGAG *F TCCCTCCGAAGCGGAGGAAGCTCGCATAGTTCGGGAAATGGAAAAACTGGTGGACCGGCATAACGAGGTGGCCGAGCTG *F ACAGAAAGATTGTCGGGTGTTATGGTGGAAATAACACTGGCCCACTTTGTTACTTCGAGTTTGATAATCGGAACCAGCG *F TGGTGGATATTTTATTATTTTCCGGCCTGGGAATCATTGTGTATGTGGTCTACACTTGTGCCGTAGGTGTGGAAATATT *F TCTATACTGTTTAGGAGGATCTCATATTATGGAAGCGTGTTCCAATCTAGCGCGCTCCACATTTTCCAGCCACTGGTAT *F GGCCACAGTGTTCGGGTCCAAAAGATGACCCTTTTGATGGTAGCTCGTGCTCAACGAGTTCTCACAATTAAAATTCCTT *F TCTTTTCCCCATCATTAGAGACTCTAACTTCGATTTTGCGCTTCACTGGATCTCTGATTGCCCTGGCAAAGTCGGTTAT *F ATAA *F *F >Or30a *F ATGGAATTGAAATCGATGGATCCGGTGGAGATGCCCATTTTTGGTAGCACTCTGAAGCTAATGAAGTTCTGGTCATATC *F TGTTTGTTCACAACTGGCGCCGCTATGTCGCAATGACTCCGTACATCATTATCAACTGTACTCAGTATGTGGATATATA *F TCTGAGCACCGAATCCTTGGACTTTATCATCAGAAATGTATACCTGGCTGTATTGTTTACCAACACGGTGGTCAGAGGT *F GTATTGTTATGCGTACAGCGGTTTAGCTACGAGCGTTTCATTAATATTTTGAAAAGCTTTTACATTGAGTTGTTGCAAT *F CAGATGACCCCATCATAAACATTTTGGTCAAGGAAACCACACGCCTATCAGTTTTAATTAGTAGGATTAATTTATTAAT *F GGGCTGCTGCACTTGCATTGGCTTTGTTACATATCCCATTTTTGGTTCGGAAAGAGTTCTGCCATATGGCATGTATTTG *F CCCACTATTGATGAATACAAATACGCATCACCTTACTACGAGATTTTCTTTGTGATTCAAGCCATTATGGCTCCAATGG *F GGTGTTGCATGTACATACCATACACAAACATGGTAGTGACATTTACCCTTTTCGCCATTCTCATGTGTCGAGTGTTGCA *F ACATAAGTTGAGAAGCCTAGAAAAGCTGAAAAATGAACAAGTACGTGGTGAAATCATATGGTGCATAAAATATCAATTA *F AAATTATCAGGATTTGTTGATTCAATGAATGCCTTGAACACCCATCTTCATTTGGTGGAGTTCCTTTGCTTTGGTGCCA *F TGCTATGTGTTCTTCTTTTCTCCTTAATAATTGCTCAAACAATTGCTCAGACCGTCATAGTCATCGCATACATGGTAAT *F GATATTTGCCAACAGTGTAGTCCTTTACTACGTGGCCAATGAGCTATACTTTCAAAGCTTTGATATTGCCATTGCTGCC *F TATGAGAGCAATTGGATGGACTTTGATGTGGACACACAAAAGACTTTGAAGTTCCTCATCATGCGCTCGCAAAAGCCCT *F TGGCGATTCTGGTGGGTGGCACATATCCCATGAACTTGAAAATGCTTCAGTCACTACTAAATGCCATTTACTCCTTCTT *F CACCCTTCTGCGTCGCGTTTACGGCTAA *F *F >Or35a *F ATGGTTCGTTACGTGCCCCGGTTCGCTGATGGTCAGAAAGTAAAGTTGGCTTGGCCCTTGGCGGTTTTTCGGTTAAATC *F ACATATTCTGGCCATTGGATCCGAGCACAGGGAAATGGGGCCGATATCTGGACAAGGTTCTAGCTGTTGCGATGTCCTT *F GGTTTTTATGCAACACAACGATGCAGAGCTGAGGTACTTGCGCTTCGAGGCAAGTAATCGGAATTTGGATGCCTTTCTC *F ACAGGAATGCCAACGTATTTAATCCTCGTGGAGGCTCAATTTAGAAGTCTTCACATTCTACTGCACTTCGAGAAGCTTC *F AGAAGTTTTTAGAAATATTCTACGCAAATATTTATATTGATCCCCGTAAGGAACCCGAAATGTTTCGAAAAGTGGATGG *F AAAGATGATAATTAACAGATTAGTTTCGGCCATGTACGGTGCAGTTATCTCTCTGTATCTAATCGCACCCGTTTTTTCC *F ATCATTAACCAAAGCAAAGATTTTCTATACTCTATGATCTTTCCGTTCGATTCGGATCCCTTGTACATATTTGTGCCAC *F TGCTTTTGACAAACGTATGGGTTGGCATTGTAATAGATACCATGATGTTCGGGGAGACGAATTTGTTGTGTGAACTAAT *F TGTCCACCTAAATGGTAGTTATATGTTGCTCAAGAGGGACTTGCAGTTGGCCATTGAAAAGATATTAGTTGCAAGGGAC *F CGTCCGCATATGGCCAAACAGCTAAAGGTTTTAATTACAAAAACTCTCCGAAAGAATGTGGCTCTAAATCAGTTTGGCC *F AGCAGCTGGAGGCTCAGTATACTGTGCGGGTTTTTATTATGTTTGCATTCGCTGCGGGCCTTTTATGTGCTCTTTCTTT *F TAAGGCTTATACGAATCCTATGGCTAATTACATCTATGCGATTTGGTTTGGTGCTAAGACAGTTGAGCTGCTTTCTTTA *F GGACAGATTGGTTCCGACTTGGCCTTTACTACGGATTCCCTCAGCACAATGTACTACCTTACCCATTGGGAGCAAATCC *F TGCAGTACTCTACAAATCCCAGCGAAAATCTGCGATTACTAAAGCTCATTAACTTGGCCATTGAGATGAACAGCAAGCC *F CTTCTATGTGACAGGGCTAAAATATTTTCGCGTTAGTCTGCAGGCTGGCTTAAAAATTCTGCAGGCATCCTTCTCGTAC *F TTCACATTCCTCACTTCGATGCAGCGACGACAAATGAGCAAT *F *F >Or42a *F ATGGATCTGCGAAGGTGGTTTCCGACCTTGTACACCCAGTCGAAGGATTCGCCAGTTCGCTCCCGAGACGCGACCCTGT *F ACCTCCTACGCTGCGTCTTCTTAATGGGCGTCCGCAAGCCACCTGCCAAGTTTTTCGTGGCCTACGTGCTCTGGTCCTT *F CGCACTGAATTTCTGCTCAACATTTTATCAGCCAATTGGCTTTCTCACAGGCTATATAAGCCATTTATCAGAGTTCTCC *F CCGGGAGAGTTTCTAACTTCGCTGCAGGTGGCCTTTAATGCTTGGTCCTGCTCTACAAAAGTCCTGATAGTGTGGGCAC *F TAGTTAAGCGCTTTGACGAGGCTAATAACCTTCTCGACGAGATGGATAGGCGTATCACAGACCCCGGAGAGCGTCTTCA *F GATTCATCGCGCTGTCTCCCTCAGTAACCGTATATTCTTCTTTTTCATGGCAGTCTACATGGTTTATGCCACTAATACG *F TTTCTGTCGGCGATCTTCATTGGAAGGCCACCGTACCAAAATTACTACCCTTTTCTGGACTGGCGATCTAGCACTCTGC *F ATCTAGCTCTGCAGGCCGGTCTGGAATACTTCGCCATGGCTGGCGCCTGCTTCCAGGACGTTTGCGTTGATTGCTACCC *F AGTCAATTTCGTTTTGGTCCTGCGTGCCCACATGTCGATCTTCGCGGAGCGCCTTCGACGTTTGGGAACTTATCCTTAT *F GAAAGCCAGGAGCAGAAATATGAACGATTGGTTCAGTGCATACAAGATCACAAAGTAATTTTGCGATTTGTTGACTGCC *F TGCGTCCTGTTATTTCTGGTACCATCTTCGTGCAATTCTTGGTTGTGGGGTTGGTGCTGGGCTTTACCCTAATTAACAT *F TGTCCTGTTCGCCAACTTGGGATCGGCCATCGCAGCGCTCTCGTTTATGGCCGCAGTGCTTCTAGAGACGACTCCCTTC *F TGCATATTGTGCAATTATCTCACAGAAGACTGCTACAAGCTGGCCGATGCCCTGTTTCAGTCAAACTGGATTGATGAGG *F AGAAACGATACCAAAAGACACTCATGTACTTCCTACAGAAACTGCAGCAGCCTATAACCTTCATGGCTATGAACGTGTT *F TCCAATATCTGTGGGAACTAACATCAGTGTCACAAAATTTTCGTTCTCCGTCTTTACTCTCGTAAAACAAATGAACATA *F TCTGAGAAACTTGCCAAATCTGAAATGGAAGAGTGA *F *F >Or42b *F ATGGTCTTCGAGCTAATACGTCCCGCTCCGCTCACGGAGCAGAAGCGGTCCCGAGATGGTTGCATCTACCTTTACCGCG *F CCATGAAGTTTATTGGATGGCTGCCCCCCAAGCAGGGTGTGCTCCGGTATGTGTACCTCACCTGGACGCTAATGACGTT *F CGTGTGGTGTACAACGTACCTGCCGCTTGGCTTCCTTGGTAGCTACATGACGCAGATCAAGTCCTTCTCCCCTGGAGAG *F TTTCTCACTTCACTCCAGGTGTGCATTAATGCCTACGGCTCATCGGTAAAAGTTGCAATCACATACTCCATGCTCTGGC *F GCCTTATCAAGGCCAAGAACATTTTGGACCAGCTGGACCTGCGCTGCACCGCCATGGAGGAGCGCGAAAAGATCCACCT *F AGTGGTGGCCCGCAGCAACCATGCCTTTCTCATCTTCACCTTTGTCTACTGCGGATATGCCGGCTCCACCTACCTGAGC *F TCGGTTCTCAGCGGGCGTCCGCCCTGGCAGCTGTACAATCCCTTTATTGATTGGCATGACGGCACACTCAAGCTCTGGG *F TGGCCTCCACGTTGGAGTACATGGTGATGTCAGGCGCCGTTCTGCAGGATCAACTCTCGGACTCTTACCCATTGATCTA *F TACCCTCATCCTTCGTGCTCACTTGGACATGCTAAGGGAGCGCATCCGACGCCTCCGTTCCGATGAGAACCTGAGCGAG *F GCCGAGAGCTATGAAGAGCTGGTCAAATGTGTGATGGACCACAAGCTCATTCTAAGATACTGCGCGATTATTAAACCAG *F TAATCCAGGGGACCATCTTCACACAGTTTCTGCTGATCGGCCTGGTTCTGGGCTTCACGCTGATCAACGTGTTTTTCTT *F CTCAGACATCTGGACGGGCATCGCATCATTTATGTTTGTTATAACCATTTTGCTGCAGACCTTCCCCTTCTGCTACACA *F TGCAACCTCATCATGGAGGACTGCGAGTCCTTGACCCATGCTATTTTCCAGTCCAACTGGGTGGATGCCAGTCGTCGCT *F ACAAAACAACACTACTGTATTTTCTCCAAAACGTGCAGCAGCCTATCGTTTTCATTGCAGGCGGTATCTTTCAGATATC *F CATGAGCAGCAACATAAGTGTGGCAAAGTTTGCTTTCTCCGTGATAACCATTACCAAGCAAATGAATATAGCTGACAAA *F TTTAAGACGGACTAA *F *F >Or43b *F ATGTTCGGACACTTTAAGCTCGTCTATCCGGCTCCTATATCGGAGCCCATACAGTCTAGGGATTCGAATGCATACATGA *F TGGAGACGCTGCGAAATTCGGGCTTGAATTTGAAGAACGATTTCGGTATAGGCCGCAAGATTTGGAGGGTGTTTTCGTT *F CACCTACAATATGGTGATACTTCCCGTAAGTTTCCCAATCAACTATGTGATACATCTGGCGGAGTTCCCGCCGGAGCTG *F CTGCTGCAATCCCTGCAACTGTGCCTCAACACTTGGTGCTTCGCTCTGAAGTTCTTCACTCTGATCGTCTATACGCACC *F GCTTGGAGCTGGCCAACAAGCACTTTGACGAATTGGATAAGTACTGCGTGAAGCCGGCGGAGAAGCGCAAGGTTCGCGA *F CATGGTGGCCACTATTACAAGACTGTACCTGACCTTCGTCGTGGTCTACGTCCTCTACGCCACCTCCACGCTACTGGAC *F GGACTACTGCACCACCGTGTTCCCTACAATACGTACTATCCGTTCATAAACTGGCGAGTCGATCGGACCCAGATGTACA *F TCCAGAGTTTTCTGGAGTACTTCACCGTGGGTTATGCCATATATGTGGCCACCGCCACCGATTCCTACCCTGTGATTTA *F CGTGGCAGCCCTGCGAACTCATATTCTCTTGCTCAAGGACCGTATCATTTACTTGGGCGATCCCAGCAACGAGGGTAGC *F AGCGACCCGAGCTACATGTTTAAATCGTTGGTGGATTGTATCAAGGCACACAGAACCATGCTAAATTTTTGTGATGCCA *F TTCAACCAATCATCTCTGGCACGATATTTGCCCAATTCATCATATGCGGATCGATCCTGGGCATAATTATGATCAACAT *F GGTATTGTTCGCTGATCAATCGACCCGATTCGGCATAGTCATCTACGTTATGGCCGTCCTTCTGCAGACTTTTCCGCTT *F TGCTTCTACTGCAACGCCATCGTGGACGACTGCAAAGAACTGGCCCACGCACTTTTCCATTCCGCCTGGTGGGTGCAGG *F ACAAGCGATACCAGCGGACTGTCATCCAGTTCCTGCAGAAACTGCAGCAGCCCATGACCTTCACCGCCATGAACATATT *F TAACATTAATTTGGCCACTAACATCAATGTAGCCAAGTTCGCCTTCACCGTGTACGCCATCGCGAGCGGTATGAACCTG *F GACCAAAAGTTAAGCATTAAGGAATAG *F *F >Or46a *F ATGAGCAAAGGAGTAGAAATCTTTTACAAGGGCCAGAAGGCATTCTTGAACATCCTCTCGTTGTGGCCTCAGATAGAAC *F GCCGGTGGAGAATCATCCACCAGGTGAACTATGTCCACGTAATTGTGTTTTGGGTGCTGCTCTTTGATCTCCTCTTGGT *F GCTCCATGTGATGGCTAATTTGAGCTACATGTCCGAGGTTGTGAAAGCCATCTTTATCCTGGCCACCAGTGCAGGGCAC *F ACCACCAAGCTGCTGTCCATAAAGGCGAACAATGTGCAGATGGAGGAGCTCTTTAGGAGATTGGATAACGAAGAGTTCC *F GTCCTAGAGGCGCCAACGAAGAGTTGATCTTTGCAGCAGCCTGTGAAAGAAGTAGGAAGCTTCGGGACTTCTATGGAGC *F GCTTTCGTTTGCCGCCTTGAGCATGATTCTCATACCCCAGTTCGCCTTGGACTGGTCCCACCTTCCGCTCAAAACATAC *F AATCCGCTTGGCGAGAATACCGGCTCACCTGCTTATTGGCTCCTCTACTGCTATCAGTGTCTGGCCTTGTCCGTATCCT *F GCATCACCAACATAGGATTCGACTCACTCTGCTCCTCACTGTTCATCTTCCTCAAGTGCCAGCTGGACATTCTGGCCGT *F GCGACTGGACAAGATCGGTCGGTTAATCACTACTTCTGGTGGCACTGTGGAACAGCAACTTAAGGAAAATATCCGCTAT *F CACATGACCATCGTTGAACTGTCGAAAACCGTGGAGCGTCTACTTTGCAAGCCGATTTCGGTGCAGATCTTCTGCTCGG *F TTTTGGTGCTGACTGCCAATTTCTATGCCATTGCTGTGTTATCTGACGAGAGGCTGGAGCTCTTTAAGTATGTGACCTA *F TCAGGCGTGCATGTTGATTCAGATTTTTATATTGTGCTACTATGCCGGTGAGGTAACCCAGCGCAGCCTGGACCTTCCG *F CACGAGCTGTACAAGACCTCCTGGGTGGACTGGGACTACAGGAGCCGAAGGATTGCGCTCCTCTTTATGCAACGCCTTC *F ACTCGACCTTGAGGATTAGGACACTTAATCCAAGTCTTGGTTTTGACTTAATGCTCTTCAGCTCGGTGAGTTCTTTCCG *F TGTTTTGACTTTTTTGTGCACTGTAGCCAATTTCCATAATGAGGCTCATTAG *F *F >Or46b *F ATGGTTACGGAGGACTTTTATAAGTACCAGGTGTGGTACTTCCAAATCCTTGGTGTTTGGCAGCTCCCCACTTGGGCCG *F CAGACCACCAGCGTCGTTTTCAGTCCATGAGGTTTGGCTTCATCCTGGTCATCCTGTTCATCATGCTGCTGCTTTTCTC *F CTTCGAAATGTTGAACAACATTTCCCAAGTTAGGGAGATCCTAAAGGTATTCTTCATGTTCGCCACGGAAATATCCTGC *F ATGGCCAAATTATTGCATTTGAAGTTGAAGAGCCGCAAACTCGCTGGCTTGGTTGATGCGATGTTGTCCCCAGAGTTCG *F GCGTTAAAAGTGAACAGGAAATGCAGATGCTGGAATTGGATAGAGTGGCGGTTGTCCGCATGAGGAACTCCTACGGCAT *F CATGTCCCTGGGCGCGGCTTCCCTGATCCTTATAGTTCCCTGTTTCGACAACTTTGGCGAGCTACCACTGGCCATGTTG *F GAGGTATGCAGCATCGAGGGATGGATCTGCTATTGGTCGCAGTACCTTTTCCACTCGATTTGCCTGCTGCCCACTTGTG *F TGCTGAATATAACCTACGACTCGGTGGCCTACTCGTTGCTCTGTTTCTTGAAGGTTCAGCTACAAATGCTGGTCCTGCG *F ATTAGAAAAGTTGGGTCCTGTGATCGAACCCCAGGATAATGAGAAAATCGCAATGGAACTGCGTGAGTGTGCCGCCTAC *F TACAACAGGATTGTTCGTTTCAAGGACCTGGTGGAGCTGTTCATAAAGGGGCCAGGATCTGTGCAGCTCATGTGTTCTG *F TTCTGGTGCTGGTGTCCAACCTGTACGACATGTCCACCATGTCCATTGCAAACGGCGATGCCATCTTTATGCTCAAGAC *F CTGTATCTATCAGCTGGTGATGCTCTGGCAGATCTTCATCATTTGCTACGCCTCCAACGAGGTAACTGTCCAGAGCTCT *F AGGTTGTGTCACAGCATCTACAGCTCCCAATGGACGGGATGGAACAGGGCAAACCGCCGGATTGTCCTTCTCATGATGC *F AGCGCTTTAATTCCCCGATGCTCCTGAGCACCTTTAACCCCACCTTTGCTTTCAGCTTGGAGGCCTTTGGTTCTATCGT *F CAACTGCTCCTACAGCTACTTCGCACTGCTGAAGCGCGTCAACAGTTAA *F *F *F >Or56a *F ATGTTTAAAGTTAAGGATCTGTTGCTTTCGCCGACAACTTTCGAGGATCCAATTTTTGGAACCCACCTGCGATACTTCC *F AATGGTACGGATATGTGGCCTCCAAGGATCAGAATAGGCCTTTGTTAAGTCTTATACGGTGCACCATTTTGACGGCATC *F GATTTGGCTTAGCTGTGCTTTAATGCTGGCGAGAGTGTTTCGTGGTTACGAAAACCTCAATGATGGGGCCACAAGTTAC *F GCCACCGCAGTCCAGTATTTCGCGGTATCGATTGCCATGTTTAATGCTTACGTACAAAGAGATAAAGTAATATCCCTTT *F TGCGAGTTGCCCACTCGGATATCCAGAACTTGATGCACGAAGCAGATAATCGGGAGATGGAACTTTTGGTCGCCACTCA *F GGCTTATACACGAACCATTACCCTGTTGATCTGGATACCATCGGTTATTGCTGGCCTAATGGCCTATTCAGACTGCATC *F TACAGGAGTCTGTTTCTGCCGAAATCGGTTTTCAATGTGCCAGCTGTGCGACGTGGTGAGGAGCATCCCATTCTGCTAT *F TTCAGCTGTTTCCCTTCGGAGAACTTTGCGATAACTTCGTTGTTGGATACTTGGGACCTTGGTATGCTCTGGGCCTGGG *F AATCACGGCTATCCCATTGTGGCACACCTTTATCACTTGCCTCATGAAGTACGTAAATCTCAAGCTGCAAATACTCAAC *F AAGCGAGTGGAGGAGATGGATATTACCCGACTTAATTCCAAATTGGTAATTGGTCGCCTAACTGCCAGTGAGTTAACCT *F TCTGGCAAATGCAACTCTTCAAGGAATTTGTAAAGGAACAGCTGAGGATTCGAAAATTTGTCCAGGAACTACAGTATCT *F GATTTGCGTGCCTGTGATGGCAGATTTCATTATCTTCTCGGTTCTCATTTGCTTTCTCTTTTTTGCCTTGACAGTTGGC *F GTTCCAAGCAAAATGGATTACTTCTTCATGTTCATTTACCTTTTTGTGATGGCTGGTATATTGTGGATTTATCATTGGC *F ATGCCACGTTGATTGTTGAATGTCACGATGAACTGAGCCTTGCTTACTTTTCTTGCGGATGGTACAACTTCGAAATGCC *F TTTGCAGAAAATGCTGGTTTTTATGATGATGCATGCCCAAAGGCCGATGAAGATGCGCGCCCTGCTGGTCGATTTGAAT *F CTGAGGACCTTCATAGACATTGGCCGTGGAGCCTACAGCTACTTCAATTTGCTGCGTAGCTCCCACTTGTATTAG *F *F >Or63a *F ATGTACTCACCGGAAGAGGCGGCCGAACTGAAGAGGCGCAACTATCGCAGCATCAGGGAGATGATCCGACTCTCCTATA *F CGGTGGGCTTCAACCTGTTGGATCCTTCCCGATGCGGACAGGTGCTCAGAATCTGGACAATTGTCCTTAGCGTGAGTAG *F CTTGGCATCGCTTTATGGGCACTGGCAAATGTTAGCCAGGTACATTCATGATATTCCACGCATTGGAGAGACCGCTGGA *F ACTGCCCTGCAGTTCCTAACATCGATAGCAAAGATGTGGTACTTTCTGTTTGCCCATAGACAGATATACGAATTGCTAC *F GAAAGGCGCGCTGCCATGAATTACTCCAAAAGTGTGAGCTCTTTGAAAGGATGTCAGATCTACCTGTTATCAAAGAGAT *F TCGCCAGCAGGTTGAGTCCACGATGAATCGGTACTGGGCCAGCACTCGTCGGCAAATTCTTATCTATTTGTACAGCTGT *F ATTTGTATTACTACAAACTACTTTATCAACTCCTTCGTAATCAACCTCTATCGCTATTTCACTAAACCGAAAGGATCCT *F ACGACATAATGTTACCTCTGCCATCTCTGTATCCCGCCTGGGAGCACAAGGGATTAGAGTTTCCCTACTATCATATACA *F GATGTACCTGGAAACCTGTTCTCTGTATATCTGCGGCATGTGTGCCGTTAGCTTTGATGGAGTCTTTATTGTCCTGTGC *F CTTCATAGCGTGGGACTTATGAGGTCACTTAACCAAATGGTGGAACAAGCCACATCTGAGTTGGTTCCTCCAGATCGCA *F GGGTTGAATACTTGCGATGCTGTATTTATCAGTACCAACGAGTGGCGAACTTTGCAACCGAGGTTAACAACTGCTTTCG *F GCACATCACTTTCACGCAGTTCCTGCTTAGCCTTTTCAACTGGGGCCTGGCCTTGTTCCAAATGAGCGTCGGATTGGGC *F AACAACAGCAGCATCACCATGATCCGGATGACCATGTACCTGGTGGCAGCCGGCTATCAGATAGTTGTGTACTGCTACA *F ATGGCCAGCGATTTGCGACTGCTAGCGAGGAGATTGCCAACGCCTTTTACCAGGTGCGATGGTACGGAGAGTCCAGGGA *F GTTCCGCCACCTCATCCGCATGATGCTGATGCGCACGAACCGGGGATTCAGGCTGGACGTGTCCTGGTTCATGCAAATG *F TCCTTGCCCACACTGATGGCGATGGTCCGGACAAGTGGACAGTACTTCCTGCTGCTGCAGAACGTCAACCAGAAATAG *F *F >Or65a *F ATGACCGAGCTGCGGAGTGAGCGGAAAAATGGTAATTGGGATCGATTGTTTGGACCGTTTTTCGAAAGTTGGGCTGTTT *F TCAAGGCTCCCCAGGCGAAGTCCCGACACATTATCGCCTACTGGACTCGGGACCAGTTGAAAGCCCTAGGTTTCTACAT *F GAACTCTGAGCAACGCCGTTTGCCCAGGATTGTGGCCTGGCAATATTTTGTTTCGATCCAACTGGCAACGGCACTGGCA *F TCTCTGTTTTATGGTATCAGTGAATCCATCGGTGATATTGTCAATCTGGGACGAGATCTGGTCTTTATAATAACGATCA *F TATTTATTTGCTTCAGACTGGTGTTCTTTGCTCAATATGCTGGTGAATTGGATGTCATAATCGATGCTCTCGAGGATAT *F TTATCATTGGAGTATAAAAGGCCCTGCAACAAAGGAAGTGCAAGAAACCAAGCGTTTGCATTTCCTATTGTTTATGGCC *F TTGATCATTACCTGGTTCAGCTTTCTCATACTTTTTATGCTGATAAAAATATCCACACCATTTTGGATTGAATCGCAGA *F CATTACCCTTTCACGTCTCTTGGCCCTTTCAACTTCACGATCCATCGAAACATCCGATTGCCTATATCATCATCTTTGT *F ATCCCAAAGCACCACGATGTTGTATTTCCTGATTTGGCTTGGTGTTGTTGAGAATATGGGTGTGTCTTTATTTTTTGAG *F CTAACTTCTGCTCTAAGGGTTTTATGCATCGAACTAAGAAATCTTCAGGAACTTTGCCTGGGCGATGAAGATATGCTGT *F ACAGAGAGCTTTGTCGAATGACCAAGTTCCATCAGCAAATCATACTACTTACAGATCGCTGCAACCATATATTCAATGG *F AGCTTTTATCATGCAAATGTTGATTAACTTTCTGCTCGTCTCATTGTCACTGTTCGAAGTGCTGGCAGCCAAGAAGAAT *F CCCCAAGTCGCAGTCGAGTATATGATTATTATGTTAATGACTCTGGGTCACCTGTCATTCTGGTCCAAATTCGGAGATA *F TGTTTTCCAAAGAATCGGAGCAAGTAGCTTTGGCTGTCTACGAAGCGTATGACCCGAATGTTGGATCCAAATCAATCCA *F CCGACAGTTTTGTTTCTTCATTCAAAGGGCTCAAAAGCCATTAATCATGAAGGCATCTCCATTTCCACCTTTTAATTTA *F GAAAACTATATGTTTATTCTAAAGCAGTGCTATTCAATTTTGACCATATTAGCAAACACGTTGGAGTAG *F *F >Or65b *F ATGGAGGCTAGCCATTCCTCAATTTACTATTGGCGGGAGCAGATGAAAGCAATGGCCTTATTTACAACCACAGAAGAAC *F GTCTGCTGCCCTACCGATCTAAATGGCACACCTTGGTATATATTCAAATGGTTATATTTTTTGCTTCAATGAGCTTTGG *F CTTAACGGAATCGATGGGAGACCATGTTCAAATGGGACGGGACTTAGCCTTCATCCTTGGGGCTTTTTTTATTATATTC *F AAGACATATTATTTCTGCTGGTATGGCGATGAACTTGACCAAGTGATCAGCGATCTGGACGCTCTACATCCTTGGGCAC *F AGAAAGGTCCTAATCCAGTTGAATATCAGACTGGTAAACGTTGGTACTTCGTAATGGCTTTTTTCTTGGCAACGTCATG *F GTCGTTCTTCTTGTGCATTTTGCTATTGTTACTTATAACCTCACCCATGTGGGTCCATCAGCAAAACCTTCCCTTTCAT *F GCGGCGTTTCCTTTTCAATGGCACGAAAAATCGCTTCATCCCATCAGCCACGCTATAATCTATCTGTTTCAGAGCTATT *F TTGCAGTGTATTGTCTGACTTGGCTTTTGTGCATAGAGGGACTATCAATTTGTATTTATGCGGAAATTACTTTCGGCAT *F TGAAGTTTTATGCCTAGAACTACGCCAAATTCACCGACACAATTATGGCCTTCAAGAACTGAGAATGGAGACGAACCGC *F TTGGTCAAGCTACATCAGAAGATTGTAGAAATTTTAGATCGTACCAACGACGTTTTTCACGGAACTCTCATAATGCAGA *F TGGGTGTTAACTTTTCCTTGGTGTCCTTGTCGGTTTTGGAGGCCGTGGAGGCTCGGAAGGACCCCAAAGTTGTGGCCCA *F GTTTGCAGTCCTTATGTTGCTCGCCTTAGGACATCTATCTATGTGGTCGTATTGTGGAGACCAGTTATCCCAGAAGTCA *F TTGCAAATTTCGGAGGCTGCCTATGAGGCTTACGACCCAACCAAAGGATCAAAGGATGTGTATAGAGACCTCTGCGTAA *F TAATCAGGCGTGGCCAGGACCCTTTGATCATGAGAGCCAGCCCATTTCCGTCCTTTAATTTAATAAACTACAGCGCTAT *F ACTTAACCAATGTTATGGAATCCTGACATTTTTGCTAAAGACATTAGACTAA *F *F >Or65c *F ATGGAGTCCAGCTACTCCGCAGTTTACTATTGGAGAGAGCAGATGAAAGCAATGTTCTTATATACAACCTCAAAAGAAC *F GTCAAATGCCCTATCGTTCCTCTTGGCACACTTTGGTTATCATTCAAGCAACCGTGTGTTTTTTAACAATGTGCTATGG *F AGTTACCGAATCGTTAGGAGACAAGGTTCAAATGGGTCGGGATATTGCTTTTATCATTGGGTTCTTCTATATTGCTTTT *F AAAATATACTATTTCCAATGGTATGGCGATGAACTTGACGAGGTTGTTGAAGCTCTGGAGACATTTCATCCTTGGGCAC *F AAAAAGGTCCTGGTGCAGTGGATTATAGAACTGCCAAACGCTGGTATTTCACGTTGGCATTTTTTTTAGCTTCATCTTG *F GCTGGTGTTCCTGTGTATTTTTATATTGCTGCTCATAACATCACCGCTGTGGGTTCATCAGCAGATCCTTCCTCTTCAT *F GCGGCTTTTCCATTCCAATGGCACGAAAAGTCGATTCACCCCATCAGCCACGCATTTATCTATTTGTTTCAGACTTGGA *F ATGTAATGTATTTTCTGACTTGGCTTGTGTGCATTGAAGGACTCTCTGTATCTATTTACGTGGAAATAACATTTGCCAT *F CGAAGTTCTATGCCTCGAACTGCGTCACCTTCATCAGAGGTGCCATGGATATGAGCAGTTGAGATTGGAGACGAATCGC *F CTGGTCCAGTTCCATCAGAAGATTGTTCACATCTTAGATCACACTAACAAAGTTTTTCATGGAACCCTCATAATGCAAA *F TGGGGGTTAACTTTTTCCTGGTGTCCCTATCGGTTCTAGAGGCCATGGAGGCAAGAAAAGACCCCAAGGTGGTGGCCCA *F GTTTGCCGTCCTCATGCTGCTAGCCTTGGGACATCTATCCATGTGGTCGTATTTTGGAGACCTGTTATCGCAGAAATCC *F TTGACAATTTCAGAGGCTGCTTATGAGGCGTATGATCCCATCAAAGGATCCAAGGATGTTTATAGAGACCTCTGCCTAA *F TAATTCGGCGTGGCCAGGAGCCTCTGATTATGAGAGCCAGCCCCTTTCCGTCCTTTAATTTCATAAACTACAGCGCTAT *F ACTTAACCAATGCTATGGAATCTTGACATTTCTGCTAAAGACATTAGACTGA *F *F >Or67a *F ATGGATAACGTCGCGGAAATGCCTGAAGAAAAGTATGTCGAAGTCGATGATTTTTTGAGGCTAGCTGTGAAATTCTACA *F ATACTTTGGGCATTGATCCCTATGAAACTGGACGAAAACGAACTATTTGGTTTCAAATATATTTCGCATTGAATATGTT *F TAATATGGTGTTTAGTTTTTATGCCGAGGTAGCGACTCTGGTGGACAGGTTACGCGATAATGAAAATTTTCTCGAGAGC *F TGCATCTTACTGAGCTACGTGTCCTTTGTGGTCATGGGCCTCTCCAAGATAGGTGCTGTAATGAAAAAAAAGCCAAAAA *F TGACAGCTTTGGTCAGGCAATTGGAGACCTGCTTTCCGTCGCCAAGTGCAAAGGTTCAAGAGGAATATGCTGTGAAGTC *F CTGGCTGAAACGCTGCCATATATACACAAAGGGATTTGGTGGTCTCTTCATGATCATGTATTTCGCTCACGCTCTGATT *F CCCTTATTCATATACTTCATTCAAAGAGTGCTGCTCCACTATCCGGATGCCAAGCAGATTATGCCGTTTTACCAACTCG *F AACCTTGGGAATTTCGCGACTCCTGGTTGTTTTATCCAAGCTATTTTCACCAGTCGTCGGCCGGATATACGGCTACATG *F TGGATCCATTGCCGGTGACCTAATGATCTTCGCTGTGGTCCTGCAGGTCATCATGCACTACGAAAGACTGGCCAAGGTT *F CTTAGGGAGTTTAAGATTCAAGCCCATAACGCACCCAATGGAGCTAAGGAGGATATAAGGAAGTTGCAGTCCCTAGTCG *F CCAATCACATTGATATACTTCGACTCACTGATCTGATGAACGAGGTCTTTGGAATTCCCTTGTTGCTAAACTTTATTGC *F ATCTGCGCTGCTGGTCTGCCTGGTGGGAGTTCAATTAACCATCGCTTTAAGTCCAGAGTATTTTTGCAAGCAGATGCTA *F TTTCTGATTTCCGTACTGCTTGAGGTCTATCTCCTTTGCTCCTTCAGCCAGAGGTTAATAGATGCTAGCGAAAACGTGG *F GCCATGCGGCATACGATATGGATTGGTTAGGTTCCGACAAACGATTCAAGAAAATTTTAATTTTTATATCTATGCGATC *F CCAGAAGCCAGTTTGCCTTAAAGCCACAGTTGTCTTGGACTTATCCATGCCAACTATGAGCATCTTTCTTGGTATGTCG *F TATAAGTTTTTCTGCGCTGTGAGGACTATGTATCAATAA *F *F >Or67b *F ATGCAGGACCAACTGGATCACGAGCTGGAGCGGATCGACAAGCTGCCAAAACTGGGGCTTCTCTGGGTGGAGTACAGTG *F CCTATGCACTGGGAGTTAATATTGCACCGAGGAAGCGTAGCTCGAAATACTGTCGATTAACTCGCATTTTGGTGTTGAT *F TGTTAACTTGAGTATAATCTACAGTCTCGTCGCCTTTATTATGGAGAACTATATGATCTCCTTCGAGACCTACGTTGAG *F GCTGTTTTGCTTACCTTTCAATTAAGCGTTGGTGTGGTTAAGATGTTTCACTTTCAGAACAAAGTGGAATCGTGTTCCC *F AACTAGTGTTTTCTACGGAGACTGGAGAGGTATTAAAGTCCCTGGGTCTTTTTCAATTGGATTTGCCGAGAAAAAAGGA *F ACTGCTGAGCTCAGTTAGTTTGATTTTGCTTAACAATTGGATGATAATCGATCGTCAGGTGATGTTCTTCTTCAAGATC *F GTTTGCATGCCTGTTCTATACTATTGTGTGCGACCATACTTTCAATATATCTTCGATTGCTATATCAAGGACAAGGATA *F CCTGTGAAATGACCTTGACTTACCCGGCAATTGTGCCTTATTTGCAATTGGGAAATTATGAATTTCCTTCCTATGTGAT *F TCGCTTCTTTTTACTTCAATCTGGTCCTCTTTGGTGCTTTTTTGCCGTTTTTGGCTTTAACAGCCTTTTCGTGGTCCTT *F ACCAGATATGAGTCTGGTCTGATAAAAGTCCTAAGGTTTCTGGTCCAAAATTCCACCTCGGATATCCTGGTTCCCAAGG *F ACCAAAGAGTTAAATATCTTCAATGTTGCGTGAGGCTTTTTGCTCGCATATCAAGCCATCATAATCAAATTGAAAACCT *F CTTCAAGTATATCATCCTGGTTCAGTGTTCCGTTAGCAGTATTTTGATCTGCATGCTGCTCTACAAGATCAGCACGGTT *F TTGGAAGTGGGCTGGGTGTGGATGGGCATGATTATGGTCTACTTTGTGACCATAGCTCTGGAGATCACTCTGTACAACG *F TGAGTGCACAGAAGGTGGAGAGCCAGAGTGAACTGCTGTTCCATGACTGGTACAACTGTAGTTGGTATAATGAGTCAAG *F GGAATTCAAGTTCATGATCAAAATGATGCTGCTTTTCTCACGCCGAACTTTTGTTTTAAGCGTGGGTGGGTTTACAAGT *F CTCTCCCACAAGTTCCTAGTGCAGGTCTTTCGGTTGAGTGCAAACTTCTTTCTGCTCCTGCGCAACATGAACAACAAAT *F AG *F *F >Or67c *F ATGGAAACAGCGAAGGATAATACAGCCAGGACTTTTATGGAATTGATGCGAGTGCCAGTACAGTTTTACAGAACGATTG *F GAGAGGATATCTACGCCCATCGATCCACGAATCCCCTAAAATCGCTTCTCTTCAAGATCTATCTATATGCGGGATTCAT *F AAATTTTAATCTGTTGGTAATCGGTGAACTGGTGTTCTTCTACAACTCAATTCAGGACTTTGAAACCATTCGATTGGCC *F ATCGCGGTGGCTCCATGTATCGGATTTTCTCTGGTTGCTGATTTTAAACAAGCTGCCATGATTAGAGGCAAGAAAACAC *F TAATTATGCTACTCGATGATTTGGAGAACATGCATCCGAAAACCCTGGCAAAGCAAATGGAATACAAATTGCCGGACTT *F TGAAAAGACCATGAAACGTGTGATCAATATATTCACCTTTCTCTGCTTGGCCTATACGACTACGTTCTCCTTTTATCCG *F GCCATCAAGGCATCCGTGAAATTTAATTTCTTGGGCTACGACACCTTTGATCGAAATTTTGGTTTCCTCATCTGGTTTC *F CCTTCGATGCAACAAGGAATAATTTGATATACTGGATCATGTACTGGGACATAGCCCATGGGGCCTATCTAGCGGGTAT *F TGCTTTTCTCTGCGCCGATCTTTTGCTCGTCGTAGTCATTACCCAGATTTGTATGCACTTTAACTATATATCTATGCGA *F TTAGAGGATCATCCATGTAATTCGAATGAGGACAAAGAGAATATAGAGTTTCTTATTGGCATTATCAGATACCATGACA *F AGTGCCTTAAACTATGCGAACATGTCAACGATCTGTATAGTTTCTCTTTGCTGCTTAATTTCCTTATGGCATCCATGCA *F GATTTGTTTCATAGCCTTTCAGGTCACCGAATCAACAGTGGAAGTGATTATTATTTACTGCATTTTTTTGATGACCTCG *F ATGGTTCAGGTATTTATGGTGTGCTACTATGGGGATACTTTAATTGCCGCGAGCTTGAAAGTGGGCGATGCCGCTTACA *F ACCAAAAGTGGTTTCAGTGCAGCAAATCCTATTGCACCATGTTGAAGTTGCTAATCATGAGGAGTCAGAAACCAGCTTC *F AATAAGACCGCCGACTTTTCCCCCCATATCCTTGGTTACCTATATGAAGGTCATCAGCATGTCGTATCAATTTTTTGCC *F TTACTTAGAACCACATACAGCAATAATTGA *F *F >Or67d *F ATGTTGAAAATGGCAAAAGTTGAGCCTGTAGAGCGCTATTGTAAAGTCATTCGAATGATTCGCTTTTGCGTGGGATTTT *F GCGGAAACGATGTGGCAGATCCCAATTTTCGGATGTGGTGGCTCACCTACGCTGTGATGGCAGCCATTGCCTTCTTCTT *F CGCCTGTACAGGATATACAATTTATGTGGGAGTGGTCATCAATGGAGACCTCACCATAATCCTACAGGCATTGGCCATG *F GTCGGCTCCGCCGTTCAGGGACTTACTAAACTTTTGGTAACCGCCAATAATGCTTCTCACATGCGAGAAGTTCAGAATA *F CCTACGAGGATATCTATAGAGAATACGGATCTAAGGGAGATGAATATGCTAAATGCTTGGAGAAGCGAATCCGCATTAC *F ATGGACTCTTTTAATCGGCTTCATGCTCGTCTACATTATTCTTTTGGGCCTTGTAATCACCTTTCCCATATTCTACTTG *F CTGATTCTGCACCAAAAGGTATTGGTCATGCAATTTCTAATACCATTCCTCGATCACACAACAGATGGTGGTCACCTAA *F TACTCACGGCTGCTCATGTGATTTTAATTACATTCGGTGGATTTGGTAACTACGGAGGGGATATGTACCTATTTCTTTT *F CGTTACCCATGTGCCGTTAATCAAGGACATATTCTGTGTGAAGCTTACGGAATTTAACGAGTTGGTTATGAAAAGGAAT *F GATTTTCCAAAAGTGAGAGCCATGCTTTGCGACCTGTTGGTCTGGCATCAGCTGTATACTAGAATGCTTCAAACCACCA *F AGAAGATCTATTCCATAGTCTTGTTCGTTCAACTTTCCACAACTTGCGTAGGTCTTCTCTGTACCATATCTTGCATCTT *F TATGAAAGCCTGGCCCGCAGCTCCTCTTTACCTTTTATATGCCGCCATAACTCTCTACACCTTTTGCGGCTTGGGTACT *F TTAGTAGAAAATTCAAATGAGGATTTCCTAAGTGTGATCTACACGAATTGTTTGTGGTATGAGCTACCCGTTAAGGAGG *F AGAAACTTATTATCATGATGCTGGCCAAGGCTCAAAATGAAGTTGTACTCACTGCAGCCGACATGGCGCCTTTGTCGAT *F GAACACAGCTTTGCAATTGACGAAAGGCATATATAGTTTCAGCATGATGTTGATGAACTATTTGGGATAA *F *F >Or69b *F ATGCAGTTGCACGACCATATGAAGTACATAGACTTGGGTTGCAAGATGGCATGCATACCAAGATATCAATGGAAAGGAC *F GCCCTACTGAAAGACAGTTCTACGCTTCGGAGCAAAGGATAGTGTTCCTTCTTGGAACCATTTGCCAGATATTCCAGAT *F TACTGGAGTGCTTATCTATTGGTATTGCAATGGCCGTCTTGCCACGGAAACGGGCACCTTTGTGGCACAATTATCTGAA *F ATGTGCAGTTCTTTTTGTCTAACATTTGTGGGATTCTGTAACGTTTATGCGATCTCTACAAACCGCAATCAAATTGAAA *F CATTACTCGAGGAGCTTCATCAGATATATCCGAGATACAGGAAAAATCACTATCGCTGCCAGCATTATTTTGACATGGC *F CATGACAATAATGAGAATTGAGTTTCTTTTCTATATGATCTTGTACGTGTACTACAATAGTGCACCATTATGGGTGCTT *F CTTTGGGAACACTTGCACGAGGAATATGATCTTAGCTTCAAGACGCAGACCAACACTTGGTTTCCATGGAAAGTCCATG *F GGTCGGCACTTGGATTTGGTATGGCTGTACTAAGCATAACCGTGGGATCCTTTGTGGGCGTAGGTTTCAGTATTGTCAC *F CCAGAATCTTATCTGTTTGTTAACCTTCCAACTAAAGTTGCACTACGATGGAATATCCAGTCAGTTAGTATCTCTCGAT *F TGCCGTCGTCCTGGAGCTCATAAGGAGTTGAGCATCCTCATCGCCCACCACAGCCGAATCCTTCAGCTGGGCGACCAAG *F TCAATGACATAATGAACTTTGTATTCGGCTCTAGCCTAGTAGGTGCCACTATTGCCATTTGTATGTCAAGTGTTTCTAT *F AATGCTACTGGACTTAGCATCTGCCTTCAAATATGCCAGTGGTCTAGTGGCATTCGTCCTCTACAACTTTGTCATCTGC *F TACATGGGAACCGAGGTCACTTTAGCT *F *F >Or69a *F ATGCAGTTGGAGGACTTTATGCGGTACCCGGACCTCGTGTGTCAAGCGGCCCAACTTCCCAGATACACGTGGAATGGCA *F GACGATCCTTGGAAGTTAAACGCAACTTGGCAAAACGCATTATCTTCTGGCTTGGAGCAGTAAATTTGGTTTATCACAA *F TATTGGCTGCGTCATGTATGGCTATTTCGGTGATGGAAGAACAAAGGATCCAATTGCGTATTTAGCTGAATTGGCATCT *F GTGGCCAGCATGCTTGGTTTCACCATTGTGGGCACCCTCAACTTGTGGAAGATGCTGAGCCTTAAGACCCATTTTGAGA *F ACCTACTAAATGAATTCGAGGAATTATTTCAACTAATCAAGCACAGGGCGTATCGCATACACCACTATCAAGAAAAGTA *F TACGCGTCATATACGAAATACATTTATTTTCCATACCTCTGCCGTTGTCTACTACAACTCACTACCAATTCTTCTAATG *F ATTCGGGAACATTTCTCGAACTCACAGCAGTTGGGCTATAGAATTCAGAGTAATACCTGGTATCCCTGGCAGGTTCAGG *F GATCAATTCCTGGATTTTTTGCTGCAGTCGCCTGTCAAATCTTTTCGTGCCAAACCAATATGTGCGTCAATATGTTTAT *F CCAGTTTCTGATCAACTTTTTTGGTATCCAGCTAGAAATACACTTCGATGGTTTGGCCAGGCAGCTGGAGACCATCGAT *F GCCCGCAATCCCCATGCCAAGGATCAATTGAAGTATCTGATTGTATATCACACAAAATTGCTTAATCTAGCCGACAGAG *F TTAATCGATCGTTTAACTTTACGTTTCTCATAAGTCTGTCGGTATCCATGATATCCAACTGTTTTCTGGCATTTTCCAT *F GACCATGTTCGACTTTGGCACCTCTCTAAAACATTTACTCGGACTTTTGCTATTCATCACATATAATTTTTCAATGTGC *F CGCAGTGGTACGCACTTGATTTTAACGAGTGGCAAAGTATTGCCAGCGGCCTTTTATAACAATTGGTATGAAGGCGATC *F TTGTTTATCGAAGGATGCTCCTCATCCTGATGATGCGTGCTACGAAACCTTATATGTGGAAAACCTACAAGCTGGCACC *F TGTATCCATAACTACATATATGGCAACATTGAAGTTTTCATATCAAATGTTTACCTGTGTGCGGTCCCTTAAATAA *F *F >Or71a *F ATGGACTACGATCGAATTCGACCGGTGCGATTTTTGACGGGAGTGCTGAAATGGTGGCGTCTCTGGCCGAGGAAGGAAT *F CGGTGTCCACACCGGACTGGACTAACTGGCAGGCATATGCCTTGCACGTTCCATTTACATTCTTGTTTGTGTTGCTTTT *F GTGGTTGGAGGCAATCAAGAGCAGGGATATACAGCATACCGCCGATGTCCTTTTGATTTGCCTAACCACCACTGCCTTG *F GGAGGTAAAGTTATCAATATCTGGAAGTATGCCCATGTGGCCCAAGGCATTTTGTCCGAGTGGAGCACGTGGGATCTTT *F TCGAGCTGAGGAGCAAACAGGAAGTGGATATGTGGCGATTCGAGCATCGACGTTTCAATCGTGTTTTTATGTTTTACTG *F TTTGTGCAGTGCTGGTGTAATCCCATTTATTGTGATTCAACCGTTGTTTGATATCCCAAATCGATTGCCCTTCTGGATG *F TGGACACCATTCGATTGGCAGCAGCCTGTTCTCTTTTGGTATGCATTCATCTATCAGGCCACAACCATTCCTATTGCCT *F GTGCTTGCAACGTAACCATGGACGCTGTTAATTGGTACTTGATGCTGCATCTGTCCTTGTGTTTGCGTATGTTGGGCCA *F GCGATTGAGTAAGCTTCAGCATGATGACAAGGATCTGAGGGAGAAGTTCCTGGAACTGATCCATCTGCACCAGCGACTC *F AAGCAACAGGCCTTGAGCATTGAAATCTTTATTTCGAAGAGCACGTTCACCCAAATTCTGGTCAGTTCCCTTATCATTT *F GCTTCACCATTTACAGCATGCAGATGAGTCCCGTCTTGCAGGACTTGCCAGGATTTGCCGCCATGATGCAGTACCTAGT *F GGCCATGATCATGCAGGTCATGCTGCCCACCATATATGGTAACGCCGTCATCGATTCTGCAAATATGTTGACCGATTCC *F ATGTACAATTCGGATTGGCCGGATATGAATTGCCGAATGCGTCGCCTAGTTTTAATGTTTATGGTGTACTTAAATCGAC *F CGGTGACCTTAAAAGCCGGTGGCTTTTTTCATATTGGTTTACCTCTGTTTACCAAGACCATGAATCAAGCATACAGTTT *F GCTGGCCTTGCTGCTCAACATGAACCAATAG *F *F >Or82a *F ATGGGTAGGCTGTTTCAACTGCAAGAATACTGCCTACGGGCTATGGGGCATAAGGATGACATGGATAGTACCGATAGTA *F CCGCACTCAGCTTGAAGCACATTAGCTCCCTGATCTTCGTAATATCGGCGCAGTATCCCCTGATCTCTTATGTCGCCTA *F CAACCGCAATGACATGGAAAAGGTCACGGCATGCCTCAGCGTGGTGTTCACCAATATGTTAACGGTTATAAAAATTTCT *F ACATTTTTGGCAAACAGAAAGGACTTTTGGGAAATGATTCACCGCTTCAGAAAAATGCATGAACAGTCAGCAAGTCACA *F TTCCTAGGTACCGAGAGGGATTGGACTACGTTGCCGAAGCCAACAAACTAGCATCTTTTCTAGGAAGAGCATATTGCGT *F GTCCTGCGGCCTAACAGGACTCTATTTTATGCTGGGACCAATTGTGAAAATTGGGGTTTGCCGTTGGCATGGCACAACA *F TGTGACAAGGAGTTGCCCATGCCAATGAAGTTTCCATTTAACGATCTGGAAAGCCCCGGATACGAAGTTTGTTTCCTCT *F ACACCGTACTAGTCACTGTCGTCGTTGTTGCCTACGCCTCGGCAGTCGATGGCTTATTTATTTCGTTTGCCATTAATCT *F GCGAGCTCATTTCCAAACACTGCAGAGGCAAATTGAAAACTGGGAGTTTCCTTCATCAGAACCAGACACACAGATCAGG *F CTGAAATCAATCGTTGAATACCACGTGCTACTCCTATCCCTATCCAGGAAGTTACGATCGATATACACACCCACCGTTA *F TGGGGCAGTTCGTCATAACCTCCTTGCAGGTTGGCGTTATAATATACCAACTAGTGACTAACATGGACTCTGTCATGGA *F TCTCTTGCTGTATGCATCGTTTTTTGGTTCAATCATGCTGCAATTATTTATCTATTGCTACGGCGGTGAAATCATCAAG *F GCTGAGAGTCTACAAGTCGATACTGCTGTTCGGCTCTCCAATTGGCATCTGGCTTCTCCCAAGACACGTACATCTTTGT *F CATTGATCATTCTTCAGTCTCAAAAGGAAGTGCTTATTAGGGCTGGCTTCTTTGTTGCATCTCTGGCTAATTTCGTTGG *F GATCTGTCGAACAGCCCTATCGTTGATTACCCTCATCAAATCTATTGAGTAA *F *F >Or83a *F ATGAAGAGCACATTCAAGGAAGAAAGGATTAAGGACGACTCCAAGCGTCGCGACCTGTTTGTATTCGTGAGGCAAACCA *F TGTGTATAGCGGCCATGTATCCCTTCGGTTACTACGTGAATGGATCTGGAGTCCTGGCCGTTCTGGTGCGATTCTGTGA *F CTTGACCTACGAGCTCTTTAACTACTTCGTTTCGGTACACATAGCTGGCCTGTACATCTGCACCATCTACATCAACTAT *F GGGCAAGGCGATTTGGACTTCTTCGTGAACTGTTTGATACAAACCATTATTTATCTGTGGACAATAGCGATGAAACTCT *F ACTTTCGGAGGTTCAGACCTGGTTTGTTGAATACCATTCTGTCCAACATCAATGATGAGTACGAGACACGTTCGGCTGT *F GGGATTCAGTTTCGTCACAATGGCGGGATCCTATCGGATGTCCAAGCTATGGATCAAAACCTATGTGTATTGCTGCTAC *F ATAGGCACCATTTTCTGGCTGGCTCTTCCCATTGCCTACCGGGATAGGAGTCTTCCTCTTGCCTGCTGGTATCCCTTTG *F ACTATACACAACCCGGTGTCTATGAGGTAGTGTTCCTTCTCCAGGCGATGGGACAGATCCAAGTGGCCGCATCCTTTGC *F CTCCTCCAGTGGCCTGCATATGGTGCTTTGTGTGCTGATATCAGGGCAGTACGATGTCCTCTTTTGCAGTCTCAAGAAT *F GTATTAGCCAGCAGCTATGTCCTTATGGGAGCCAATATGACGGAACTGAATCAATTGCAGGCTGAGCAATCTGCGGCCG *F ATGTCGAGCCAGGTCAGTATGCTTACTCCGTGGAGGAGGAGACACCTTTGCAAGAACTTCTAAAAGTTGGGAGCTCAAT *F GGACTTCTCCTCCGCATTCAGGCTGTCTTTTGTGCGGTGCATTCAGCACCATCGATACATAGTGGCGGCACTGAAGAAA *F ATTGAGAGTTTCTACAGTCCCATATGGTTCGTGAAGATTGGCGAAGTCACCTTTCTTATGTGCCTGGTAGCCTTCGTCT *F CCACGAAGAGCACCGCGGCCAACTCATTCATGCGAATGGTCTCCTTGGGCCAGTACCTGCTCTTAGTTCTCTACGAGCT *F GTTCATCATCTGCTACTTCGCGGACATCGTTTTTCAGAACAGCCAGCGGTGCGGTGAAGCCCTCTGGCGAAGTCCTTGG *F CAGCGACATTTGAAGGATGTTCGCAGTGATTACATGTTCTTTATGCTGAATTCCCGCAGGCAGTTCCAACTTACGGCCG *F GAAAAATAAGCAATCTAAACGTGGATCGTTTCAGAGGGACTATTACTACTGCCTTCTCGTTTCTCACCTTGCTGCAAAA *F GATGGATGCACGAGAATAA *F *F >Or83b *F ATGACAACCTCGATGCAGCCGAGCAAGTACACGGGCCTGGTCGCCGACCTGATGCCCAACATCCGGGCGATGAAGTACT *F CCGGCCTGTTCATGCACAACTTCACGGGCGGCAGTGCCTTCATGAAGAAGGTGTACTCCTCCGTGCACCTGGTGTTCCT *F CCTCATGCAGTTCACCTTCATCCTGGTCAACATGGCCCTGAACGCCGAGGAGGTCAACGAGCTGTCGGGCAACACGATC *F ACGACCCTCTTCTTCACCCACTGCATCACGAAGTTTATCTACCTGGCTGTTAACCAGAAGAATTTCTACAGAACATTGA *F ATATATGGAACCAGGTGAACACGCATCCCTTGTTCGCCGAGTCGGATGCTCGTTACCATTCGATCGCACTGGCGAAGAT *F GAGGAAGCTGTTCTTTCTGGTGATGCTGACCACAGTCGCCTCGGCCACCGCCTGGACCACGATCACCTTCTTTGGCGAC *F AGCGTAAAAATGGTGGTGGACCATGAGACGAACTCCAGCATCCCGGTGGAGATACCCCGGCTGCCGATTAAGTCCTTCT *F ACCCGTGGAACGCCAGCCACGGCATGTTCTACATGATCAGCTTTGCCTTTCAGATCTACTACGTGCTCTTCTCGATGAT *F CCACTCCAATCTATGCGACGTGATGTTCTGCTCTTGGCTGATATTCGCCTGCGAGCAGCTGCAGCACTTGAAGGGCATC *F ATGAAGCCGCTGATGGAGCTGTCCGCCTCGCTGGACACCTACAGGCCCAACTCGGCGGCCCTCTTCAGGTCCCTGTCGG *F CCAACTCCAAGTCGGAGCTAATTCATAATGAAGAAAAGGATCCCGGCACCGACATGGACATGTCGGGCATCTACAGCTC *F GAAAGCGGATTGGGGCGCTCAGTTTCGAGCACCCTCGACACTGCAGTCCTTTGGCGGGAACGGGGGCGGAGGCAACGGG *F TTGGTGAACGGCGCTAATCCCAACGGGCTGACCAAAAAGCAGGAGATGATGGTGCGCAGTGCCATCAAGTACTGGGTCG *F AGCGGCACAAGCACGTGGTGCGACTGGTGGCTGCCATCGGCGATACTTACGGAGCCGCCCTCCTCCTCCACATGCTGAC *F CTCGACCATCAAGCTGACCCTGCTGGCATACCAGGCCACCAAAATCAACGGAGTGAATGTCTACGCCTTCACAGTCGTC *F GGATACCTAGGATACGCGCTGGCCCAGGTGTTCCACTTTTGCATCTTTGGCAATCGTCTGATTGAAGAGAGTTCATCCG *F TCATGGAGGCCGCCTACTCGTGCCACTGGTACGATGGCTCCGAGGAGGCCAAGACCTTCGTCCAGATCGTGTGCCAGCA *F GTGCCAGAAGGCGATGAGCATATCGGGAGCGAAATTCTTCACCGTCTCCCTGGATTTGTTTGCTTCGGTTCTGGGTGCC *F GTCGTCACCTACTTTATGGTGCTGGTGCAGCTCAAGTAA *F *F >Or83c *F ATGAGTACTTCTGAAAGTCCTTCAAGTCGCTTTCGCGAACTATCCAAGTACATCAATAGTTTGACTAATTTACTTGGCG *F TGGATTTTCTTTCGCCCAAATTGAAATTTAATTATCGCACCTGGACCACGATCTTTGCAATCGCCAACTATACTGGCTT *F CACGGTATTTACTATACTAAATAATGGCGGTGACTGGAGGGTGGGACTGAAGGCCAGTCTTATGACTGGTGGGCTATTC *F CACGGACTCGGGAAGTTCCTGACTTGTCTGCTGAAGCATCAAGACATGAGACGTCTGGTCCTTTACTCACAAAGCATTT *F ACGATGAATACGAGACTCGGGGAGATTCCTACCACAGGACCTTGAATTCGAACATAGATCGACTCCTCGGCATCATGAA *F GATCATTCGAAACGGATATGTATTCGCTTTTTGTTTGATGGAACTCCTGCCATTGGCCATGCTAATGTACGATGGAACC *F CGGGTTACTGCGATGCAGTATTTAATTCCGGGTCTACCGCTTGAGAACAATTATTGCTACGTAGTCACGTACATGATTC *F AGACGGTGACAATGCTCGTGCAAGGAGTCGGATTCTACTCCGGTGATTTGTTCGTATTTCTCGGCTTAACGCAGATCCT *F AACTTTCGCCGATATGCTGCAGGTGAAGGTGAAAGAGCTAAACGATGCCCTGGAACAAAAAGCGGAATACAGAGCTCTA *F GTCCGAGTTGGAGCTTCTATTGATGGAGCGGAAAATCGTCAACGCCTTCTCTTGGATGTTATAAGATGGCATCAATTAT *F TCACGGACTACTGTCGCGCCATAAATGCCCTCTACTACGAATTGATCGCCACTCAGGTTCTTTCGATGGCTTTGGCCAT *F GATGCTCAGCTTCTGCATTAATTTGAGCAGCTTTCACATGCCTTCGGCTATCTTTTTCGTGGTTTCTGCCTACAGCATG *F TCCATCTATTGCATTCTGGGCACCATTCTTGAGTTTGCATATGACCAGGTGTACGAGAGCATCTGTAATGTGACCTGGT *F ATGAGTTGAGTGGCGAACAGCGAAAGCTTTTTGGTTTTTTGTTGCGGGAATCCCAGTATCCGCACAATATTCAGATACT *F TGGAGTTATGTCGCTTTCCGTGAGAACGGCTCTGCAGATTGTTAAACTAATTTATAGCGTATCCATGATGATGATGAAT *F CGGGCGTAG *F *F >Or85b *F ATGGAGAAGCTAATGAAGTACGCTAGCTTCTTCTACACAGCAGTGGGCATACGGCCATATACCAATGGTGAAGAATCCA *F AAATGAACAAACTTATATTTCACATAGTTTTTTGGTCCAATGTGATTAACCTGAGCTTCGTTGGATTATTTGAGAGCAT *F TTACGTTTACAGTGCCTTCATGGATAATAAGTTCCTGGAAGCAGTCACTGCGTTGTCCTACATTGGCTTCGTAACCGTA *F GGCATGAGCAAGATGTTCTTCATCCGGTGGAAGAAAACGGCTATAACTGAACTGATTAATGAATTGAAGGAGATCTATC *F CGAATGGTTTGATCCGAGAGGAAAGATACAATCTGCCGATGTATCTGGGCACCTGCTCCAGAATCAGCCTTATATATTC *F CTTGCTCTACTCTGTTCTCATCTGGACATTCAACTTGTTTTGTGTAATGGAGTATTGGGTCTATGACAAGTGGCTCAAC *F ATTCGAGTGGTGGGCAAACAGTTGCCGTACCTCATGTACATTCCTTGGAAATGGCAGGATAACTGGTCGTACTATCCAC *F TGTTATTCTCCCAGAATTTTGCAGGATACACATCTGCAGCTGGTCAAATTTCAACCGATGTCTTGCTCTGCGCGGTGGC *F CACTCAGTTGGTAATGCACTTCGACTTTCTCTCAAATAGTATGGAACGCCACGAATTGAGTGGAGATTGGAAGAAGGAC *F TCCCGATTTCTGGTGGACATTGTTAGGTATCACGAACGTATACTCCGCCTTTCAGATGCAGTGAACGATATATTTGGAA *F TTCCACTACTACTCAACTTCATGGTATCCTCGTTCGTCATCTGCTTCGTGGGATTCCAGATGACTGTTGGAGTTCCGCC *F GGATATAGTTGTGAAGCTCTTCCTCTTCCTTGTCTCTTCGATGAGTCAGGTCTATTTGATTTGTCACTATGGTCAACTG *F GTGGCCGATGCTAGCTACGGATTTTCGGTTGCCACCTACAATCAGAAGTGGTATAAAGCCGATGTGCGCTATAAACGAG *F CCTTGGTTATTATTATAGCTAGATCGCAGAAGGTAACTTTTCTAAAGGCCACTATATTCTTGGATATTACCAGGTCCAC *F TATGACAGATCTGCTTCAAATATCATACAAATTCTTCGCCCTGCTGCGCACAATGTATACCCAATAG *F *F >Or85c *F ATGAAGTTCATGAAGTACGCAGTTTTCTTTTACACATCGGTGGGCATTGAGCCGTATACGATTGACTCGCGGTCCAAAA *F AAGCGAGCCTATGGTCACATCTTCTCTTCTGGGCCAATGTGATCAATTTAAGTGTCATTGTTTTCGGAGAGATCCTCTA *F TCTGGGAGTGGCCTATTCCGATGGAAAGTTCATTGATGCCGTCACTGTACTGTCATATATCGGATTCGTAATCGTGGGC *F ATGAGCAAGATGTTCTTCATATGGTGGAAGAAGACCGATCTAAGCGATTTGGTTAAGGAATTGGAGCACATCTATCCAA *F ATGGCAAAGCTGAGGAGGAGATGTATCGGTTGGATAGGTATCTGCGATCTTGTTCACGAATTAGCATTACCTATGCACT *F ACTCTACTCCGTACTCATCTGGACCTTCAATCTGTTCAGTATCATGCAATTCCTTGTCTATGAAAAGTTGCTTAAAATC *F CGAGTGGTCGGCCAAACGCTGCCATATTTGATGTACTTTCCCTGGAACTGGCATGAAAACTGGACGTATTATGTGCTGC *F TGTTCTGTCAAAACTTCGCAGGACATACTTCGGCATCGGGACAGATCTCTACGGATCTTTTGCTTTGTGCTGTTGCTAC *F CCAGGTGGTAATGCACTTCGATTACTTGGCCAGAGTGGTGGAAAAACAAGTGTTAGATCGCGATTGGAGCGAAAACTCC *F AGATTTTTGGCAAAAACTGTACAATATCATCAGCGCATTCTTCGGCTAATGGACGTTCTCAACGATATATTCGGGATAC *F CGCTACTGCTTAACTTTATGGTCTCCACATTTGTCATCTGCTTTGTGGGATTCCAAATGACCGTGGGTGTCCCGCCGGA *F CATCATGATTAAGCTCTTCTTGTTCCTGTTCTCGTCCTTGTCGCAAGTGTACTTGATATGCCACTACGGCCAGCTGATT *F GCCGATGCGAGCTCTAGCTTATCGATTTCTGCATATAAGCAGAATTGGCAAAATGCTGACATTCGCTATCGTCGGGCTC *F TGGTATTCTTTATAGCTCGACCTCAGAGGACAACTTATCTAAAAGCTACAATTTTCATGAATATAACAAGGGCCACCAT *F GACGGACCTTCTTCAAGTATCCTACAAATTTTTCGCTCTGCTTCGTACCATGTACATAAAGTAA *F *F *F >Or98a *F ATGTTGTTCAACTATCTGCGAAAGCCGAATCCGACAAACCTTTTGACTTCTCCGGACTCATTTAGATACTTTGAGTATG *F GAATGTTTTGCATGGGATGGCACACACCAGCAACGCATAAGATAATCTACTATATAACATCCTGTTTGATTTTTGCTTG *F GTGTGCCGTATACTTGCCAATCGGAATCATCATTAGTTTCAAAACGGATATTAACACATTCACACCGAATGAACTGTTG *F ACAGTTATGCAATTATTTTTCAATTCAGTGGGAATGCCATTCAAGGTTCTGTTCTTCAATTTGTATATTTCTGGATTTT *F ACAAGGCCAAAAAGCTCCTTAGCGAAATGGACAAACGTTGCACCACTTTGAAGGAGCGAGTGGAAGTGCACCAAGGTGT *F GGTCCGTTGCAACAAGGCCTACCTCATTTACCAGTTCATTTATACCGCGTACACTATTTCAACATTTCTATCGGCGGCT *F CTTAGTGGAAAATTGCCATGGCGCATCTATAATCCTTTTGTGGATTTTCGAGAAAGTAGATCCAGTTTTTGGAAAGCTG *F CCCTCAACGAGACAGCACTTATGCTATTTGCTGTGACTCAAACCCTAATGAGTGATATATATCCACTGCTTTATGGTTT *F GATCCTGAGAGTTCACCTCAAACTTTTGCGACTAAGAGTGGAGAGCCTGTGCACAGATTCTGGAAAAAGCGATGCTGAA *F AACGAGCAAGATTTGATTAAGTGCATCAAGGATCACAATCTCATTATTGACTATGCTGCAGCAATACGACCAGCGGTTA *F CCCGCACAATTTTCGTTCAATTCCTCTTGATCGGAATTTGCCTTGGCCTTTCAATGATCAATCTACTCTTCTTTGCCGA *F CATCTGGACAGGATTGGCCACAGTGGCTTACATCAATGGTCTAATGGTGCAGACATTTCCATTTTGCTTCGTTTGTGAT *F CTACTCAAAAAGGATTGTGAACTTCTTGTGTCGGCCATATTTCATTCCAACTGGATTAATTCAAGCCGCAGTTACAAGT *F CATCTTTGAGATATTTTCTGAAGAACGCCCAGAAATCAATTGCTTTTACAGCCGGCTCTATTTTTCCCATTTCTACTGG *F CTCGAATATTAAGGTGGCTAAGCTGGCATTTTCGGTGGTTACTTTTGTCAATCAACTTAACATAGCTGACAGATTGACA *F AAGAACTGA *F *F >Or98b *F ATGCTGACGGACAAGTTCCTCCGACTGCAGTCCGCTTTATTTCGCCTTCTCGGACTCGAATTGTTGCACGAGCAGGATG *F TTGGCCATCGATATCCTTGGCGCAGCATCTGCTGCATTCTCTCGGTGGCCAGTTTCATGCCCCTGACCATTGCGTTTGG *F CCTGCAAAACGTCCAAAATGTGGAGCAATTAACCGACTCACTCTGCTCGGTTCTCGTGGATTTGCTGGCCCTGTGCAAA *F ATCGGGCTTTTCCTTTGGCTTTACAAGGACTTCAAGTTCCTAATAGGGCAGTTCTATTGTGTTTTGCAAACGGAAACCC *F ACACCGCTGTCGCTGAAATGATAGTGACCAGGGAAAGTCGTCGGGATCAGTTCATCAGTGCTATGTATGCCTACTGTTT *F CATTACGGCTGGCCTTTCGGCCTGCCTGATGTCCCCTCTATCCATGCTGATTAGCTACCACGAACAGGTGAATTGCAGC *F CGAAATTTCCATTTCCCAGTATATCCCTGGGACAATATGAAGCTGTCCAACTACATCATTTCCTATTTCTGGAATGTGT *F GTGCTGCATTGGGCGTGGCACTGCCCACCGTTTGTGTGGACACACTGTTCTGTTCTCTGAGCCATAATCTCTGTGCCCT *F ATTCCAGATTGCCAGGCACAAAATGATGCACTTTGAGGGCAGAAATACCAAAGAGACTCATGAGAACTTAAAGCACGTG *F TTTCAACTATATGCGTTGTGTTTGAACCTGGGCCATTTCTTAAACGAATATTTCAGACCGCTCATCTGCCAGTTTGTGG *F CAGCCTCACTGCACTTGTGTGTCCTGTGCTACCAACTGTCTGCCAATATCCTGCAGCCAGCGTTACTCTTCTATGCCGC *F ATTTACGGCAGCAGTTGTTGGCCAGGTGTCTATATACTGCTTCTGCGGATCGAGCATCCATTCGGAGTGTCAGCTATTT *F GGCCAGGCCATCTACGAGTCCAGCTGGCCCCATCTGCTGCAGGAAAACCTGCAGCTTGTAAGCTCCTTAAAAATTGCCA *F TGATGCGATCGAGTTTGGGATGTCCCATCGATGGTTACTTCTTCGAGGCCAATCGGGAGACGCTCATCACGATTGTGCG *F CACTGCTATATCCTATGTAACGCTACTCAGATCCCTGGCCTAG *F *F ------------------------------------------------------------------------------- *F Odorant receptor proteins FASTA: *F *F >Or1a *F MSKLIEVFLGNLWTQRFTFARMGLDLQPDKKGNVLRSPLLYCIMCLTTSFELCTVCAFMVQNRNQIVLCSEALMHGLQM *F VSSLLKMAIFLAKSHDLVDLIQQIQSPFTEEDLVGTEWRSQNQRGQLMAAIYFMMCAGTSVSFLLMPVALTMLKYHSTG *F EFAPVSSFRVLLPYDVTQPHVYAMDCCLMVFVLSFFCCSTTGVDTLYGWCALGVSLQYRRLGQQLKRIPSCFNPSRSDF *F GLSGIFVEHARLLKIVQHFNYSFMEIAFVEVVIICGLYCSVICQYIMPHTNQNFAFLGFFSLVVTTQLCIYLFGAEQVR *F LEAERFSRLLYEVIPWQNLPPKHRKLFLFPIERAQRETVLGAYFFELGRPLLVWIFRTAGSFTTLMNALYAKYETH *F *F >Or13a *F MFYSYPYKALSFPIQCVWLKLNGSWPLTESSRPWRSQSLLATAYIVWAWYVIASVGITISYQTAFLLNNLSDIIITTEN *F CCTTFMGVLNFVRLIHLRLNQRKFRQLIENFSYEIWIPNSSKNNVAAECRRRMVTFSIMTSLLACLIIMYCVLPLVEIF *F FGPAFDAQNKPFPYKMIFPYDAQSSWIRYVMTYIFTSYAGICVVTTLFAEDTILGFFITYTCGQFHLLHQRIAGLFAGS *F NAELAESIQLERLKRIVEKHNNIISFAKRLEDFFNPILLANLMISSVLICMVGFQIVTGKNMFIGDYVKFIIYISSALS *F QLYVLCENGDALIKQSTLTAQILYECQWEGSDRIEIQSFTPTTKRIRNQIWFMILCSQQPVRITAFKFSTLSLQSFTAI *F LSTSISYFTLLRSVYFDDEKKLD *F *F >Or19a *F MDISKVDSTRALVNHWRIFRIMGIHPPGKRTFWGRHYTAYSMVWNVTFHICIWVSFSVNLLQSNSLETFCESLCVTMPH *F TLYMLKLINVRRMRGQMISSHWLLRLLDKRLGCDDERQIIMAGIERAEFIFRTIFRGLACTVVLGIIYISASSEPTLMY *F PTWIPWNWRDSTSAYLATAMLHTTALMANATLVLNLSSYPGTYLILVSVHTKALALRVSKLGYGAPLPAVRMQAILVGY *F IHDHQIILRLFKSLERSLSMTCFLQFFSTACAQCTICYFLLFGNVGIMRFMNMLFLLVILTTETLLLCYTAELPCKEGE *F SLLTAVYSCNWLSQSVNFRRLLLLMLARCQIPMILVSGVIVPISMKTFTVMIKGAYTMLTLLNEIRKTSLE *F *F >Or22c *F MTDSGQPAIADHFYRIPRISGLIVGLWPQRIRGGGGRPWHAHLLFVFAFAMVVVGAVGEVSYGCVHLDNLVVALEAFCP *F GTTKAVCVLKLWVFFRSNRRWAELVQRLRAILWESRRQEAQRMLVGLATTANRLSLLLLSSGTATNAAFTLQPLIMGLY *F RWIVQLPGQTELPFNIILPSFAVQPGVFPLTYVLLTASGACTVFAFSFVDGFFICSCLYICGAFRLVQQDIRRIFADLH *F GDSVDVFTEEMNAEVRHRLAQVVERHNAIIDFCTDLTRQFTVIVLMHFLSAAFVLCSTILDIMLNTSSLSGLTYICYII *F AALTQLFLYCFGGNHVSESSAAVADVLYDMEWYKCDARTRKVILMILRRSQRAKTIAVPFFTPSLPALRSILSTAGSYI *F TLLKTFL *F *F >Or24a *F MERHYFMVPKFALSLIGFYPEQKRTVLVKLWSFFNFFILTYGCYAEAYYGIHYIPINIATALDALCPVASSILSLVKMV *F AIWWYQDELRSLIERRFYTLATQLTFLLLCCGFCTSTSYSVRHLIDNILRRTHGKDWIYETPFKMMFPDLLLRLPLYPI *F TYILVHWHGYITVVCFVGADGFFLGFCLYFTVLLLCLQDDVCDLLEVENIEKSPSEAEEARIVREMEKLVDRHNEVAEL *F TERLSGVMVEITLAHFVTSSLIIGTSVVDILLFSGLGIIVYVVYTCAVGVEIFLYCLGGSHIMEACSNLARSTFSSHWY *F GHSVRVQKMTLLMVARAQRVLTIKIPFFSPSLETLTSILRFTGSLIALAKSVI *F *F >Or30a *F MELKSMDPVEMPIFGSTLKLMKFWSYLFVHNWRRYVAMTPYIIINCTQYVDIYLSTESLDFIIRNVYLAVLFTNTVVRG *F VLLCVQRFSYERFINILKSFYIELLQSDDPIINILVKETTRLSVLISRINLLMGCCTCIGFVTYPIFGSERVLPYGMYL *F PTIDEYKYASPYYEIFFVIQAIMAPMGCCMYIPYTNMVVTFTLFAILMCRVLQHKLRSLEKLKNEQVRGEIIWCIKYQL *F KLSGFVDSMNALNTHLHLVEFLCFGAMLCVLLFSLIIAQTIAQTVIVIAYMVMIFANSVVLYYVANELYFQSFDIAIAA *F YESNWMDFDVDTQKTLKFLIMRSQKPLAILVGGTYPMNLKMLQSLLNAIYSFFTLLRRVYGZ *F *F >Or35a *F MVRYVPRFADGQKVKLAWPLAVFRLNHIFWPLDPSTGKWGRYLDKVLAVAMSLVFMQHNDAELRYLRFEASNRNLDAFL *F TGMPTYLILVEAQFRSLHILLHFEKLQKFLEIFYANIYIDPRKEPEMFRKVDGKMIINRLVSAMYGAVISLYLIAPVFS *F IINQSKDFLYSMIFPFDSDPLYIFVPLLLTNVWVGIVIDTMMFGETNLLCELIVHLNGSYMLLKRDLQLAIEKILVARD *F RPHMAKQLKVLITKTLRKNVALNQFGQQLEAQYTVRVFIMFAFAAGLLCALSFKAYTNPMANYIYAIWFGAKTVELLSL *F GQIGSDLAFTTDSLSTMYYLTHWEQILQYSTNPSENLRLLKLINLAIEMNSKPFYVTGLKYFRVSLQAGLKILQASFSY *F FTFLTSMQRRQMSN *F *F >Or42a *F MDLRRWFPTLYTQSKDSPVRSRDATLYLLRCVFLMGVRKPPAKFFVAYVLWSFALNFCSTFYQPIGFLTGYISHLSEFS *F PGEFLTSLQVAFNAWSCSTKVLIVWALVKRFDEANNLLDEMDRRITDPGERLQIHRAVSLSNRIFFFFMAVYMVYATNT *F FLSAIFIGRPPYQNYYPFLDWRSSTLHLALQAGLEYFAMAGACFQDVCVDCYPVNFVLVLRAHMSIFAERLRRLGTYPY *F ESQEQKYERLVQCIQDHKVILRFVDCLRPVISGTIFVQFLVVGLVLGFTLINIVLFANLGSAIAALSFMAAVLLETTPF *F CILCNYLTEDCYKLADALFQSNWIDEEKRYQKTLMYFLQKLQQPITFMAMNVFPISVGTNISVTKFSFSVFTLVKQMNI *F SEKLAKSEMEEZ *F *F >Or42b *F MVFELIRPAPLTEQKRSRDGCIYLYRAMKFIGWLPPKQGVLRYVYLTWTLMTFVWCTTYLPLGFLGSYMTQIKSFSPGE *F FLTSLQVCINAYGSSVKVAITYSMLWRLIKAKNILDQLDLRCTAMEEREKIHLVVARSNHAFLIFTFVYCGYAGSTYLS *F SVLSGRPPWQLYNPFIDWHDGTLKLWVASTLEYMVMSGAVLQDQLSDSYPLIYTLILRAHLDMLRERIRRLRSDENLSE *F AESYEELVKCVMDHKLILRYCAIIKPVIQGTIFTQFLLIGLVLGFTLINVFFFSDIWTGIASFMFVITILLQTFPFCYT *F CNLIMEDCESLTHAIFQSNWVDASRRYKTTLLYFLQNVQQPIVFIAGGIFQISMSSNISVAKFAFSVITITKQMNIADK *F FKTD *F *F >Or43b *F MFGHFKLVYPAPISEPIQSRDSNAYMMETLRNSGLNLKNDFGIGRKIWRVFSFTYNMVILPVSFPINYVIHLAEFPPEL *F LLQSLQLCLNTWCFALKFFTLIVYTHRLELANKHFDELDKYCVKPAEKRKVRDMVATITRLYLTFVVVYVLYATSTLLD *F GLLHHRVPYNTYYPFINWRVDRTQMYIQSFLEYFTVGYAIYVATATDSYPVIYVAALRTHILLLKDRIIYLGDPSNEGS *F SDPSYMFKSLVDCIKAHRTMLNFCDAIQPIISGTIFAQFIICGSILGIIMINMVLFADQSTRFGIVIYVMAVLLQTFPL *F CFYCNAIVDDCKELAHALFHSAWWVQDKRYQRTVIQFLQKLQQPMTFTAMNIFNINLATNINVAKFAFTVYAIASGMNL *F DQKLSIKE *F *F >Or46a *F MSKGVEIFYKGQKAFLNILSLWPQIERRWRIIHQVNYVHVIVFWVLLFDLLLVLHVMANLSYMSEVVKAIFILATSAGH *F TTKLLSIKANNVQMEELFRRLDNEEFRPRGANEELIFAAACERSRKLRDFYGALSFAALSMILIPQFALDWSHLPLKTY *F NPLGENTGSPAYWLLYCYQCLALSVSCITNIGFDSLCSSLFIFLKCQLDILAVRLDKIGRLITTSGGTVEQQLKENIRY *F HMTIVELSKTVERLLCKPISVQIFCSVLVLTANFYAIAVLSDERLELFKYVTYQACMLIQIFILCYYAGEVTQRSLDLP *F HELYKTSWVDWDYRSRRIALLFMQRLHSTLRIRTLNPSLGFDLMLFSSVSSFRVLTFLCTVANFHNEAH *F *F >Or46b *F MVTEDFYKYQVWYFQILGVWQLPTWAADHQRRFQSMRFGFILVILFIMLLLFSFEMLNNISQVREILKVFFMFATEISC *F MAKLLHLKLKSRKLAGLVDAMLSPEFGVKSEQEMQMLELDRVAVVRMRNSYGIMSLGAASLILIVPCFDNFGELPLAML *F EVCSIEGWICYWSQYLFHSICLLPTCVLNITYDSVAYSLLCFLKVQLQMLVLRLEKLGPVIEPQDNEKIAMELRECAAY *F YNRIVRFKDLVELFIKGPGSVQLMCSVLVLVSNLYDMSTMSIANGDAIFMLKTCIYQLVMLWQIFIICYASNEVTVQSS *F RLCHSIYSSQWTGWNRANRRIVLLMMQRFNSPMLLSTFNPTFAFSLEAFGSIVNCSYSYFALLKRVNS *F *F >Or49a *F MEKLRSYEDFIFMANMMFKTLGYDLFHTPKPWWRYLLVRGYFVLCTISNFYEASMVTTRIIEWESLAGSPSKIMRQGLH *F FFYMLSSQLKFITFMINRKRLLQLSHRLKELYPHKEQNQRKYEVNKYYLSCSTRNVLYVYYFVMVVMALEPLVQSCIMY *F LIGFGKADFTYKRIFPTRLTFDSEKPLGYVLAYVIDFTYSQFIVNVSLGTDLWMMCVSSQISMHLGYLANMLASIRPSP *F ETEQQDCDFLASIIKRHQLMIRLQKDVNYVFGLLLASNLFTTSCLLCCMAYYTVVEGFNWEGISYMMLFASVAAQFYVV *F SSHGQMLIDLSTNLAKAAFESKWYEGSLRYKKEILILMAQAQRPLEISARGVIIISLDTFKILMTITYRFFAVIRQTVEK *F *F >Or56a *F MFKVKDLLLSPTTFEDPIFGTHLRYFQWYGYVASKDQNRPLLSLIRCTILTASIWLSCALMLARVFRGYENLNDGATSY *F ATAVQYFAVSIAMFNAYVQRDKVISLLRVAHSDIQNLMHEADNREMELLVATQAYTRTITLLIWIPSVIAGLMAYSDCI *F YRSLFLPKSVFNVPAVRRGEEHPILLFQLFPFGELCDNFVVGYLGPWYALGLGITAIPLWHTFITCLMKYVNLKLQILN *F KRVEEMDITRLNSKLVIGRLTASELTFWQMQLFKEFVKEQLRIRKFVQELQYLICVPVMADFIIFSVLICFLFFALTVG *F VPSKMDYFFMFIYLFVMAGILWIYHWHATLIVECHDELSLAYFSCGWYNFEMPLQKMLVFMMMHAQRPMKMRALLVDLN *F LRTFIDIGRGAYSYFNLLRSSHLY *F *F >Or63a *F MYSPEEAAELKRRNYRSIREMIRLSYTVGFNLLDPSRCGQVLRIWTIVLSVSSLASLYGHWQMLARYIHDIPRIGETAG *F TALQFLTSIAKMWYFLFAHRQIYELLRKARCHELLQKCELFERMSDLPVIKEIRQQVESTMNRYWASTRRQILIYLYSC *F ICITTNYFINSFVINLYRYFTKPKGSYDIMLPLPSLYPAWEHKGLEFPYYHIQMYLETCSLYICGMCAVSFDGVFIVLC *F LHSVGLMRSLNQMVEQATSELVPPDRRVEYLRCCIYQYQRVANFATEVNNCFRHITFTQFLLSLFNWGLALFQMSVGLG *F NNSSITMIRMTMYLVAAGYQIVVYCYNGQRFATASEEIANAFYQVRWYGESREFRHLIRMMLMRTNRGFRLDVSWFMQM *F SLPTLMAMVRTSGQYFLLLQNVNQK *F *F >Or65a *F MTELRSERKNGNWDRLFGPFFESWAVFKAPQAKSRHIIAYWTRDQLKALGFYMNSEQRRLPRIVAWQYFVSIQLATALA *F SLFYGISESIGDIVNLGRDLVFIITIIFICFRLVFFAQYAGELDVIIDALEDIYHWSIKGPATKEVQETKRLHFLLFMA *F LIITWFSFLILFMLIKISTPFWIESQTLPFHVSWPFQLHDPSKHPIAYIIIFVSQSTTMLYFLIWLGVVENMGVSLFFE *F LTSALRVLCIELRNLQELCLGDEDMLYRELCRMTKFHQQIILLTDRCNHIFNGAFIMQMLINFLLVSLSLFEVLAAKKN *F PQVAVEYMIIMLMTLGHLSFWSKFGDMFSKESEQVALAVYEAYDPNVGSKSIHRQFCFFIQRAQKPLIMKASPFPPFNL *F ENYMFILKQCYSILTILANTLE *F *F >Or65b *F MEASHSSIYYWREQMKAMALFTTTEERLLPYRSKWHTLVYIQMVIFFASMSFGLTESMGDHVQMGRDLAFILGAFFIIF *F KTYYFCWYGDELDQVISDLDALHPWAQKGPNPVEYQTGKRWYFVMAFFLATSWSFFLCILLLLLITSPMWVHQQNLPFH *F AAFPFQWHEKSLHPISHAIIYLFQSYFAVYCLTWLLCIEGLSICIYAEITFGIEVLCLELRQIHRHNYGLQELRMETNR *F LVKLHQKIVEILDRTNDVFHGTLIMQMGVNFSLVSLSVLEAVEARKDPKVVAQFAVLMLLALGHLSMWSYCGDQLSQKS *F LQISEAAYEAYDPTKGSKDVYRDLCVIIRRGQDPLIMRASPFPSFNLINYSAILNQCYGILTFLLKTLD *F *F >Or65c *F MESSYSAVYYWREQMKAMFLYTTSKERQMPYRSSWHTLVIIQATVCFLTMCYGVTESLGDKVQMGRDIAFIIGFFYIAF *F KIYYFQWYGDELDEVVEALETFHPWAQKGPGAVDYRTAKRWYFTLAFFLASSWLVFLCIFILLLITSPLWVHQQILPLH *F AAFPFQWHEKSIHPISHAFIYLFQTWNVMYFLTWLVCIEGLSVSIYVEITFAIEVLCLELRHLHQRCHGYEQLRLETNR *F LVQFHQKIVHILDHTNKVFHGTLIMQMGVNFFLVSLSVLEAMEARKDPKVVAQFAVLMLLALGHLSMWSYFGDLLSQKS *F LTISEAAYEAYDPIKGSKDVYRDLCLIIRRGQEPLIMRASPFPSFNFINYSAILNQCYGILTFLLKTLD *F *F >Or67a *F MDNVAEMPEEKYVEVDDFLRLAVKFYNTLGIDPYETGRKRTIWFQIYFALNMFNMVFSFYAEVATLVDRLRDNENFLES *F CILLSYVSFVVMGLSKIGAVMKKKPKMTALVRQLETCFPSPSAKVQEEYAVKSWLKRCHIYTKGFGGLFMIMYFAHALI *F PLFIYFIQRVLLHYPDAKQIMPFYQLEPWEFRDSWLFYPSYFHQSSAGYTATCGSIAGDLMIFAVVLQVIMHYERLAKV *F LREFKIQAHNAPNGAKEDIRKLQSLVANHIDILRLTDLMNEVFGIPLLLNFIASALLVCLVGVQLTIALSPEYFCKQML *F FLISVLLEVYLLCSFSQRLIDASENVGHAAYDMDWLGSDKRFKKILIFISMRSQKPVCLKATVVLDLSMPTMSIFLGMS *F YKFFCAVRTMYQ *F *F >Or67b *F MQDQLDHELERIDKLPKLGLLWVEYSAYALGVNIAPRKRSSKYCRLTRILVLIVNLSIIYSLVAFIMENYMISFETYVE *F AVLLTFQLSVGVVKMFHFQNKVESCSQLVFSTETGEVLKSLGLFQLDLPRKKELLSSVSLILLNNWMIIDRQVMFFFKI *F VCMPVLYYCVRPYFQYIFDCYIKDKDTCEMTLTYPAIVPYLQLGNYEFPSYVIRFFLLQSGPLWCFFAVFGFNSLFVVL *F TRYESGLIKVLRFLVQNSTSDILVPKDQRVKYLQCCVRLFARISSHHNQIENLFKYIILVQCSVSSILICMLLYKISTV *F LEVGWVWMGMIMVYFVTIALEITLYNVSAQKVESQSELLFHDWYNCSWYNESREFKFMIKMMLLFSRRTFVLSVGGFTS *F LSHKFLVQVFRLSANFFLLLRNMNNK *F *F >Or67c *F METAKDNTARTFMELMRVPVQFYRTIGEDIYAHRSTNPLKSLLFKIYLYAGFINFNLLVIGELVFFYNSIQDFETIRLA *F IAVAPCIGFSLVADFKQAAMIRGKKTLIMLLDDLENMHPKTLAKQMEYKLPDFEKTMKRVINIFTFLCLAYTTTFSFYP *F AIKASVKFNFLGYDTFDRNFGFLIWFPFDATRNNLIYWIMYWDIAHGAYLAGIAFLCADLLLVVVITQICMHFNYISMR *F LEDHPCNSNEDKENIEFLIGIIRYHDKCLKLCEHVNDLYSFSLLLNFLMASMQICFIAFQVTESTVEVIIIYCIFLMTS *F MVQVFMVCYYGDTLIAASLKVGDAAYNQKWFQCSKSYCTMLKLLIMRSQKPASIRPPTFPPISLVTYMKVISMSYQFFA *F LLRTTYSNN *F *F >Or67d *F MLKMAKVEPVERYCKVIRMIRFCVGFCGNDVADPNFRMWWLTYAVMAAIAFFFACTGYTIYVGVVINGDLTIILQALAM *F VGSAVQGLTKLLVTANNASHMREVQNTYEDIYREYGSKGDEYAKCLEKRIRITWTLLIGFMLVYIILLGLVITFPIFYL *F LILHQKVLVMQFLIPFLDHTTDGGHLILTAAHVILITFGGFGNYGGDMYLFLFVTHVPLIKDIFCVKLTEFNELVMKRN *F DFPKVRAMLCDLLVWHQLYTRMLQTTKKIYSIVLFVQLSTTCVGLLCTISCIFMKAWPAAPLYLLYAAITLYTFCGLGT *F LVENSNEDFLSVIYTNCLWYELPVKEEKLIIMMLAKAQNEVVLTAADMAPLSMNTALQLTKGIYSFSMMLMNYLG *F *F >Or69b *F MQLHDHMKYIDLGCKMACIPRYQWKGRPTERQFYASEQRIVFLLGTICQIFQITGVLIYWYCNGRLATETGTFVAQLSE *F MCSSFCLTFVGFCNVYAISTNRNQIETLLEELHQIYPRYRKNHYRCQHYFDMAMTIMRIEFLFYMILYVYYNSAPLWVL *F LWEHLHEEYDLSFKTQTNTWFPWKVHGSALGFGMAVLSITVGSFVGVGFSIVTQNLICLLTFQLKLHYDGISSQLVSLD *F CRRPGAHKELSILIAHHSRILQLGDQVNDIMNFVFGSSLVGATIAICMSSVSIMLLDLASAFKYASGLVAFVLYNFVIC *F YMGTEVTLA *F *F >Or69a *F MQLEDFMRYPDLVCQAAQLPRYTWNGRRSLEVKRNLAKRIIFWLGAVNLVYHNIGCVMYGYFGDGRTKDPIAYLAELAS *F VASMLGFTIVGTLNLWKMLSLKTHFENLLNEFEELFQLIKHRAYRIHHYQEKYTRHIRNTFIFHTSAVVYYNSLPILLM *F IREHFSNSQQLGYRIQSNTWYPWQVQGSIPGFFAAVACQIFSCQTNMCVNMFIQFLINFFGIQLEIHFDGLARQLETID *F ARNPHAKDQLKYLIVYHTKLLNLADRVNRSFNFTFLISLSVSMISNCFLAFSMTMFDFGTSLKHLLGLLLFITYNFSMC *F RSGTHLILTSGKVLPAAFYNNWYEGDLVYRRMLLILMMRATKPYMWKTYKLAPVSITTYMATLKFSYQMFTCVRSLK *F *F >Or71a *F MDYDRIRPVRFLTGVLKWWRLWPRKESVSTPDWTNWQAYALHVPFTFLFVLLLWLEAIKSRDIQHTADVLLICLTTTAL *F GGKVINIWKYAHVAQGILSEWSTWDLFELRSKQEVDMWRFEHRRFNRVFMFYCLCSAGVIPFIVIQPLFDIPNRLPFWM *F WTPFDWQQPVLFWYAFIYQATTIPIACACNVTMDAVNWYLMLHLSLCLRMLGQRLSKLQHDDKDLREKFLELIHLHQRL *F KQQALSIEIFISKSTFTQILVSSLIICFTIYSMQMSPVLQDLPGFAAMMQYLVAMIMQVMLPTIYGNAVIDSANMLTDS *F MYNSDWPDMNCRMRRLVLMFMVYLNRPVTLKAGGFFHIGLPLFTKTMNQAYSLLALLLNMNQ *F *F >Or82a *F MGRLFQLQEYCLRAMGHKDDMDSTDSTALSLKHISSLIFVISAQYPLISYVAYNRNDMEKVTACLSVVFTNMLTVIKIS *F TFLANRKDFWEMIHRFRKMHEQSASHIPRYREGLDYVAEANKLASFLGRAYCVSCGLTGLYFMLGPIVKIGVCRWHGTT *F CDKELPMPMKFPFNDLESPGYEVCFLYTVLVTVVVVAYASAVDGLFISFAINLRAHFQTLQRQIENWEFPSSEPDTQIR *F LKSIVEYHVLLLSLSRKLRSIYTPTVMGQFVITSLQVGVIIYQLVTNMDSVMDLLLYASFFGSIMLQLFIYCYGGEIIK *F AESLQVDTAVRLSNWHLASPKTRTSLSLIILQSQKEVLIRAGFFVASLANFVGICRTALSLITLIKSIE *F *F >Or83a *F MKSTFKEERIKDDSKRRDLFVFVRQTMCIAAMYPFGYYVNGSGVLAVLVRFCDLTYELFNYFVSVHIAGLYICTIYINY *F GQGDLDFFVNCLIQTIIYLWTIAMKLYFRRFRPGLLNTILSNINDEYETRSAVGFSFVTMAGSYRMSKLWIKTYVYCCY *F IGTIFWLALPIAYRDRSLPLACWYPFDYTQPGVYEVVFLLQAMGQIQVAASFASSSGLHMVLCVLISGQYDVLFCSLKN *F VLASSYVLMGANMTELNQLQAEQSAADVEPGQYAYSVEEETPLQELLKVGSSMDFSSAFRLSFVRCIQHHRYIVAALKK *F IESFYSPIWFVKIGEVTFLMCLVAFVSTKSTAANSFMRMVSLGQYLLLVLYELFIICYFADIVFQNSQRCGEALWRSPW *F QRHLKDVRSDYMFFMLNSRRQFQLTAGKISNLNVDRFRGTITTAFSFLTLLQKMDARE *F *F >Or83b *F MTTSMQPSKYTGLVADLMPNIRAMKYSGLFMHNFTGGSAFMKKVYSSVHLVFLLMQFTFILVNMALNAEEVNELSGNTI *F TTLFFTHCITKFIYLAVNQKNFYRTLNIWNQVNTHPLFAESDARYHSIALAKMRKLFFLVMLTTVASATAWTTITFFGD *F SVKMVVDHETNSSIPVEIPRLPIKSFYPWNASHGMFYMISFAFQIYYVLFSMIHSNLCDVMFCSWLIFACEQLQHLKGI *F MKPLMELSASLDTYRPNSAALFRSLSANSKSELIHNEEKDPGTDMDMSGIYSSKADWGAQFRAPSTLQSFGGNGGGGNG *F LVNGANPNGLTKKQEMMVRSAIKYWVERHKHVVRLVAAIGDTYGAALLLHMLTSTIKLTLLAYQATKINGVNVYAFTVV *F GYLGYALAQVFHFCIFGNRLIEESSSVMEAAYSCHWYDGSEEAKTFVQIVCQQCQKAMSISGAKFFTVSLDLFASVLGA *F VVTYFMVLVQLK *F *F >Or83c *F MSTSESPSSRFRELSKYINSLTNLLGVDFLSPKLKFNYRTWTTIFAIANYTGFTVFTILNNGGDWRVGLKASLMTGGLF *F HGLGKFLTCLLKHQDMRRLVLYSQSIYDEYETRGDSYHRTLNSNIDRLLGIMKIIRNGYVFAFCLMELLPLAMLMYDGT *F RVTAMQYLIPGLPLENNYCYVVTYMIQTVTMLVQGVGFYSGDLFVFLGLTQILTFADMLQVKVKELNDALEQKAEYRAL *F VRVGASIDGAENRQRLLLDVIRWHQLFTDYCRAINALYYELIATQVLSMALAMMLSFCINLSSFHMPSAIFFVVSAYSM *F SIYCILGTILEFAYDQVYESICNVTWYELSGEQRKLFGFLLRESQYPHNIQILGVMSLSVRTALQIVKLIYSVSMMMMN *F RA *F *F >Or85b *F MEKLMKYASFFYTAVGIRPYTNGEESKMNKLIFHIVFWSNVINLSFVGLFESIYVYSAFMDNKFLEAVTALSYIGFVTV *F GMSKMFFIRWKKTAITELINELKEIYPNGLIREERYNLPMYLGTCSRISLIYSLLYSVLIWTFNLFCVMEYWVYDKWLN *F IRVVGKQLPYLMYIPWKWQDNWSYYPLLFSQNFAGYTSAAGQISTDVLLCAVATQLVMHFDFLSNSMERHELSGDWKKD *F SRFLVDIVRYHERILRLSDAVNDIFGIPLLLNFMVSSFVICFVGFQMTVGVPPDIVVKLFLFLVSSMSQVYLICHYGQL *F VADASYGFSVATYNQKWYKADVRYKRALVIIIARSQKVTFLKATIFLDITRSTMTDLLQISYKFFALLRTMYTQ *F *F >Or85c *F MKFMKYAVFFYTSVGIEPYTIDSRSKKASLWSHLLFWANVINLSVIVFGEILYLGVAYSDGKFIDAVTVLSYIGFVIVG *F MSKMFFIWWKKTDLSDLVKELEHIYPNGKAEEEMYRLDRYLRSCSRISITYALLYSVLIWTFNLFSIMQFLVYEKLLKI *F RVVGQTLPYLMYFPWNWHENWTYYVLLFCQNFAGHTSASGQISTDLLLCAVATQVVMHFDYLARVVEKQVLDRDWSENS *F RFLAKTVQYHQRILRLMDVLNDIFGIPLLLNFMVSTFVICFVGFQMTVGVPPDIMIKLFLFLFSSLSQVYLICHYGQLI *F ADASSSLSISAYKQNWQNADIRYRRALVFFIARPQRTTYLKATIFMNITRATMTDLLQVSYKFFALLRTMYIK *F *F >Or92a *F MLFRKRKPKSDDEVITFDELTRFPMTFYKTIGEDLYSDRDPNVIRRYLLRFYLVLGFLNFNAYVVGEIAYFIVHIMSTT *F TLLEATAVAPCIGFSFMADFKQFGLTVNRKRLVRLLDDLKEIFPLDLEAQRKYNVSFYRKHMNRVMTLFTILCMTYTSS *F FSFYPAIKSTIKYYLMGSEIFERNYGFHILFPYDAETDLTVYWFSYWGLAHCAYVAGVSYVCVDLLLIATITQLTMHFN *F FIANDLEAYEGGDHTDEENIKYLHNLVVYHARALDLSEEVNNIFSFLILWNFIAASLVICFAGFQITASNVEDIGVYFI *F FFSASLVQVFVVCYYGDEMISSSSRIGHSAFNQNWLPCSTKYKRILQFIIARSQKPASIRPPTFPPISFNTFMKVISMS *F YQFFALLRTTYYG *F *F >Or98a *F MLFNYLRKPNPTNLLTSPDSFRYFEYGMFCMGWHTPATHKIIYYITSCLIFAWCAVYLPIGIIISFKTDINTFTPNELL *F TVMQLFFNSVGMPFKVLFFNLYISGFYKAKKLLSEMDKRCTTLKERVEVHQGVVRCNKAYLIYQFIYTAYTISTFLSAA *F LSGKLPWRIYNPFVDFRESRSSFWKAALNETALMLFAVTQTLMSDIYPLLYGLILRVHLKLLRLRVESLCTDSGKSDAE *F NEQDLIKCIKDHNLIIDYAAAIRPAVTRTIFVQFLLIGICLGLSMINLLFFADIWTGLATVAYINGLMVQTFPFCFVCD *F LLKKDCELLVSAIFHSNWINSSRSYKSSLRYFLKNAQKSIAFTAGSIFPISTGSNIKVAKLAFSVVTFVNQLNIADRLT *F KN *F *F >Or98b *F MLTDKFLRLQSALFRLLGLELLHEQDVGHRYPWRSICCILSVASFMPLTIAFGLQNVQNVEQLTDSLCSVLVDLLALCK *F IGLFLWLYKDFKFLIGQFYCVLQTETHTAVAEMIVTRESRRDQFISAMYAYCFITAGLSACLMSPLSMLISYHEQVNCS *F RNFHFPVYPWDNMKLSNYIISYFWNVCAALGVALPTVCVDTLFCSLSHNLCALFQIARHKMMHFEGRNTKETHENLKHV *F FQLYALCLNLGHFLNEYFRPLICQFVAASLHLCVLCYQLSANILQPALLFYAAFTAAVVGQVSIYCFCGSSIHSECQLF *F GQAIYESSWPHLLQENLQLVSSLKIAMMRSSLGCPIDGYFFEANRETLITIVRTAISYVTLLRSLA # *U FBrf0136025 *a Walker *b J.H. *t 2001.4.25 *T personal communication to FlyBase *u *F From J.H.Walker@leeds.ac.uk Tue Apr 24 18:20:03 2001 *F Envelope-to: hb246@gen.cam.ac.uk *F Delivery-date: Tue, 24 Apr 2001 18:20:03 \+0100 *F X-Sender: bmb6jhw@bmb.leeds.ac.uk *F MIME-Version: 1.0 *F Date: Tue, 24 Apr 2001 18:15:50 \+0100 *F To: query-help@morgan.harvard.edu *F From: 'Dr J.H.Walker' *F Subject: CG1677 *F Sorry to bother you but we have found a mammalian nuclear protein that is *F obviously related to drosophila gene product CG1677. The mammalian protein *F is certainly a nuclear protein. CG1677 also has multiple predicted nuclear *F location signals although the flybase database says it is cytoplasmic. I *F tried looking for P-element insertions but I am not sure I was doing things *F right. Do I just put CG1677 into the appropriate search form after *F selecting the P element option? *F If so I guess the result i got (below) means there are no mutants of this gene? *F Any clarification you can supply would be much appreciated *F Thanks *F John *F 'No Results Found *F You asked for all P-Element Insertions with a name like 'CG1677' ' *F \--------------------------------------------------------------------------- *F | Dr. J.H. Walker | E-mail: bmb6jhw@bmb.leeds.ac.uk | *F | School of Biochemistry & | or J.H.Walker@leeds.ac.uk *F | *F | Molecular Biology | Tel: \+44 (0)113 233 3119 | *F | University of LEEDS | Fax: \+44 (0)113 233 3167 | *F | LEEDS LS2 9JT | | *F | United Kingdom | *F | | *F \--------------------------------------------------------------------------- # *U FBrf0136026 *a Dobie *b K. *c G. *d Karpen *t 2001.4.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Wed Apr 18 16:16:04 2001 *F Envelope-to: rd120@gen.cam.ac.uk *F To: karpen@salk.edu *F Subject: Helping FlyBase: Scim genes *F Hi Gary, *F I'm writing to you about the new Scim genes in your paper (Dobie et *F al.) in April's Genetics (Vol 157 number 4 page 1623). Table 3 lists *F 34 new genes.. Thing is \- I bet you know which CG genes at least some *F of these 'new' Scim genes map to. I know that you appreciate how we *F would prefer not to make new records for genes already represented by a *F CG entry in FlyBase, so if you could send me the correspondences I *F would be most grateful. The Scim-type names would override the CG *F ones, which would become synonyms. Feel free to pass this mail on to *F Kenneth if it is more reasonable for me to be asking him than you \- you *F are corresponding author so you get it first! *F all the best, *F Rachel. *F From karpen@salk.edu Wed Apr 18 21:05:14 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: Scim genes *F Hi Rachel *F This should do the trick. *F Gary *F \-- *F Dr. Gary H. Karpen *F Associate Professor *F MBVL *F The Salk Institute *F 10010 North Torrey Pines Road *F La Jolla, CA 92037 *F Ph: 858-453-4100 ext 1473 *F Fax: 858-622-0417 *F Email: karpen@salk.edu *F Web:http://pingu.salk.edu/users/Karpen_web/Karpen_lab_home.html *F Dominant modifiers of J21A inheritance in novel loci *F ______________________________________________________________________________ *F Line Dm CG# Function Hs Genes Function / Disorder *F ______________________________________________________________________________ *F Scim4 12497 receptor LDLR lipoprotein receptor/familial hyperc. *F 13758 G protein *F linked receptor CALCR calcitonin receptor/osteoporosis *F Scim5 15816 unknown \- *F Scim6 6999 unknown \- *F Scim7 13238 unknown \- *F Scim81 4004 unknown \- *F Scim82 4004 unknown \- *F Scim9 3587 unknown \- *F Scim10 14438 unknown KIAA0760 OLF-1/EBF associated zinc finger *F Scim11 1494 transporter ABCA4 Stargardt disease *F Scim121 9894 unknown \- *F Scim122 9894 unknown \- *F Scim123 9894 unknown \- *F Scim124 9894 unknown \- *F Scim125 9894 unknown \- *F Scim126 9894 unknown \- *F Scim127 9894 unknown \- *F Scim128 9894 unknown \- *F Scim131 9892 transporter ABCG1 ATP-binding cassette transporter *F Scim132 9892 transporter ABCG1 cholesterol & phospholipid transport *F Scim141 13791 unknown \- *F Scim142 13791 unknown \- *F Scim151 4026 enzyme ITPKA cellular signalling *F Scim152 4026 enzyme ITPKA cellular signalling *F Scim16 13143 unknown \- *F 6187 unknown C18B11 *F Scim17 17745 unknown \- *F Scim18 16798 unknown \- *F Scim19 9241 unknown Cdc23(pombe) DNA replication *F 9242 enzyme GMPS catalyzes amination of XMP to GMP *F Scim20 12110 enzyme PLD1 PC-specific activity (inc. mitosis) *F 8276 unknown \- *F Scim21 9397 unknown \- *F Scim22 3268 hom trans DKFZP564F013 *F factor *F Scim23 8709 DNA replication KIAA0249 *F factor *F Scim24 6751 unknown (WD40) TAFII100 *F 7704(Med)transcription MADH4 Pancreatic and intestinal cancer *F factor *F Scim25 8151 RNA pol II GTF2H1 Initiation of transcription *F tran factor *F 13941 signal transduction ARC activity-regulated cytoskeleton *F Scim26 13942 unknown \- *F 8603 motor/ AXPL *F cytoskeleton *F Scim27 10939 unknown SLC9A3R2 tyrosine kinase activator protein *F Scim28 13438 unknown \- *F Scim29 2852 chaperone PPIB cyclosporin A-mediated immunosup *F 13513 unknown \- *F Scim30 17816 unknown \- *F 10092 arginine-tRNA \- *F ligase *F Scim31 4029 transcription WHN T-cell immunodeficiency, alopecia *F factor *F Scim321 10120 malate ME1 *F dehydrogenase *F Scim322 10120 malate ME1 *F dehydrogenase *F Scim33 7682 unknown \- *F 7679 transporter ATP6N1A ATPase, H+ transporting, lysosomal *F Scim34 5557 transcription ZNF45 zinc finger protein 45 *F factor *F Scim35 15690 unknown \- *F 17838 RNA binding protein HNRPR *F Scim36 6295 enzyme PNLIP pancreatic lipase deficiency *F Scim37 12425 unknown \- *F ______________________________________________________________________________ *F cdc23 (S.pombe) *F encodes a protein of 593 amino acids *F cdc23 is essential for viability *F 22% overall identity and many structural homologies with DNA43 (MCM10) in *F S.cerevisiae *F MCM10 is required for correct initiation of DNA synthesis at chromosomal *F origins of replication # *U FBrf0136027 *a Cook *b K. *t 2001.4.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 23 17:26:42 2001 *F From: Kevin Cook *F Subject: P{His2AvT:Avic\GFP-S65T}62A insertion *F The following information was provided to the Bloomington Stock Center by *F Robert Saint, University of Adelaide (4/01). *F P{His2AvT:Avic\GFP-S65T}62A is a homozygous viable and fertile insertion *F in chromosomal subdivision 62A. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136028 *a Deal *b J. *c K. *d Cook *t 2001.4.24 *T personal communication to FlyBase *u Isolation and Characterization of Df(2L)BSC4. *F From kcook@bio.indiana.edu Tue Apr 24 23:08:55 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2L)BSC4 *F Isolation and Characterization of Df(2L)BSC4 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2L)BSC4 was isolated as a P transposase-induced male recombination event *F involving P{lacW}Rpp30k01901 and P{PZ}alpha-Adaptin06694. The deletion *F was isolated as a net-cn recombinant chromosome from the cross net1 b1 *F cn1 sp1 females x net1 P{lacW}Rpp30k01901/P{PZ}alpha-Adaptin06694 *F cn1; TMS, delta2-3 males. The Df(2L)BSC4 chromosome retains the miniwhite *F marker from the P{lacW} construct. Polytene chromosome squashes showed the *F breakpoints 21B7-C1;21C2-3. Df(2L)BSC4 failed to complement *F P{lacW}RpI135k16513 and P{lacW}U2af38k14504, Hopk14504 (a single *F P{lacW} insertion affecting adjacent genes). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136029 *a Cook *b K. *t 2001.4.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Apr 25 04:06:50 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(3L)HD1 breakpoints *F I examined polytene chromosome squashes of Df(3L)HD1 (FBab0028846) and *F found that it has the breakpoints 79D3-E1;79F2-6. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136030 *a Marsh *b L. *t 2001.4.24 *T personal communication to FlyBase *u *F X-Sender: jlmarsh@pop.uci.edu *F Date: Tue, 24 Apr 2001 08:11:25 \-0700 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: tsg2 *F Rachel, *F I think you can cite a personal correspondance that cv = tsg 2. We *F have excised a P element in the tsg2 locus and it gives the cv *F phenotype and fails to complement cv alleles, thus tsg2 = cv... *F Thanks *F Larry *F \-- *F J.Lawrence Marsh, Ph.D. *F Professor *F Dept. Developmental and Cell Biology *F University of California Irvine *F Irvine, CA 92697-2300 *F Voice: (949) 824-6677 *F FAX: (949) 824-3571 *F email: JLMarsh@UCI.edu *F http://darwin.bio.uci.edu/~marshlab/ # *U FBrf0136031 *a Nguyen *b D. *t 2001.4.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Apr 25 18:34:51 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{HZ50PL-1.1}Z25 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Duc Nguyen, Pennsylvania State University (12/00). *F P{HZ50PL-1.1}Z25 is a homozygous viable and fertile insertion on the 3rd *F chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136032 *a Umea Stock Center *b ?. *t 2001.4.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Apr 27 16:33:51 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(1)RR62 *F The following information accompanied stocks received from the Umea Stock *F Center (4/01). *F Df(1)RR62 was isolated by Vanya Rasheva following X ray mutagenesis of a *F w1118 chromosome. The breakpoints were determined cytologically as 3C;3D. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136033 *a Quinn *b W. *t 2001.4.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Apr 27 21:23:39 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: amnPS801 *F Chip Quinn, M.I.T., confirmed in correspondence (4/27/01) that amnPS801 *F is a synonym of amn1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136034 *a Orr *b W. *t 2001.4.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Apr 27 21:26:21 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: de11 *F William Orr, Southern Methodist University, confirmed in correspondence *F (4/27/01) that de11 is a synonym of fs(1)de11a. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136035 *a Deal *b J. *c K. *d Cook *t 2001.5.3 *T personal communication to FlyBase *u Isolation and Characterization of Df(2L)BSC5. *F From kcook@bio.indiana.edu Thu May 03 22:58:06 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Isolation and Characterization of Df(2L)BSC5 *F Isolation and Characterization of Df(2L)BSC5 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2L)BSC5 was isolated as a P transposase-induced male recombination event *F involving P{PZ}eIF-4a02439 and P{lacW}l(2)k09923k09923. The deletion *F was isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 *F cn1 bw1 females x P{PZ}eIF-4a02439 cn1/dpov1 *F P{lacW}l(2)k09923k09923; TMS, delta2-3 males. The Df(2L)BSC5 chromosome *F retains the miniwhite marker from the P{lacW} construct. Polytene *F chromosome squashes showed the breakpoints 26B1-2;26D1-2. Df(2L)BSC5 *F failed to complement P{lacW}Gef26k13720. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136037 *a Hirsh *b J. *c J. *d Burnette *t 2001.5.9 *T personal communication to FlyBase *u *F From jh6u@virginia.edu Wed May 09 16:51:25 2001 *F To: Rachel Drysdale (Genetics) *F From: Jay Hirsh *F Subject: Re: hono *F We have been unable to confirm the existence of a P element at the *F site upstream of the tyramine receptor reported to cause the mutant *F hono (Kutsukake et al., 2000, Gene 245(1): 31--42), in spite of *F receiving three independent batches of hono flies. We can PCR *F amplify across the supposed insertion site using genomic DNA *F isolated from individual flies from the hono stocks, indicating no P *F element at that site. We have communicated our concerns to the *F publishing authors. *F Thanks *F Jay *F Jay Hirsh and James Burnette, *F University of Virginia. # *U FBrf0136038 *a Luo *b L. *t 2001.5.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed May 02 15:42:51 2001 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Luo constructs and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Liqun Luo, Stanford University (5/01). *F In the entry for P{UAS-Cdc42.S89} (FBtp0008218), two mutant Cdc42 *F constructs are described as synonymous, an 'S89' construct and an 'L89' *F construct. *F According to Liqun Luo: *F >'These are in fact two different transgenes. The wild-type amino acid at *F >89 position is Ser. Ser to Phe change (F89) was trying to mimic a *F >dominant negative mutant in worm ras. L89 (Ser changes to Leu) was an *F >error in making that intended F89 mutation. However we later found that *F >L89 and F89 did have similar phenotypes when both transgenes are compared *F >side-by-side. According to the convention S89 should be renamed F89. So *F >we should keep L89 as it is and change S89 to F89.' *F Insertions of these and other constructs from Luo include: *F P{UAS-Cdc42.F89}3 third chromosome insertion *F P{UAS-Cdc42.V12}2 third chromosome insertion *F P{UAS-Cdc42.N17}3 second chromosome insertion *F P{UAS-Cdc42.L89}4 third chromosome insertion *F P{UAS-Drac1.L89}6 second chromosome insertion *F P{UAS-Drac1.V12}1 third chromosome insertion *F P{UAS-Drac1.N17}1 third chromosome insertion *F P{UAS-Drac1.L}3 second chromosome insertion *F All are homozygous viable and fertile insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136039 *a Umea Stock Center *b ?. *t 2001.5.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri May 11 17:38:44 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Umea stock information *F The following information was provided by the Umea Stock Center (5/01). *F 1. Df(2L)net14 and Df(2L)net-PM86A *F These deletions were isolated in the work described in Caggese et al. 1988, *F Biochem. Genet. 26: 571-584 (FBrf0047556), though they were not *F specifically mentioned. Df(2L)net14 has 21A1;21B5-6 breakpoints and *F Df(2L)net-PM86A has 21A1;21B7-8 breakpoints. *F 2. Df(2R)3-659 *F This Df was isolated by Ursula Protin and has breakpoints 59F6;60A5. *F 3. Df(2R)42490 *F This Df was isolated in the lab of Christiane Nusslein-Volhard. It has *F breakpoints 42E5-7;43C5-7. *F 4. Df(3R)89E1-2 *F This Df (FBab0024415) deletes bands 89E1-2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136328 *a Sullivan *b K. *t 2001.4.25 *T personal communication to FlyBase *u *F The following information was provided by Dr. Kathleen Sullivan in response *F to a curator query regarding the Rya-r44F[16] allele: *F *F "Regarding the 3' extent of the ryr[16] deletion, in ryr[16] 1830 bp of *F genomic sequence is deleted downstream from the k04913 insertion. There is *F also a small insertion of 19 bp from the 5' end of the k04913 P element. *F Based on the previously published sequence of the Ryr gene, the P element *F is 399 bp upstream of the start codon, so the deletion removes 1377 bp out *F of the 2847 bp second intron. " # *U FBrf0136329 *a Sullivan *b K. *t 2001.5.1 *T personal communication to FlyBase *u *F The following information was provided by Dr. Kathleen Sullivan in response *F to a curator query regarding the first exon of the Rya-r44F gene mentioned in *F FBrf0128659: *F *F "The text file attached contains the entire 310 bp 5'UTR sequence (plus the *F ATG start codon for 313 bp total) of the ryr gene that I determined from *F screening the GH cDNA library and 5'RACE. Given the low abundance and large *F size of the transcript, it's possible that there's more 5'UTR that I failed *F to detect. Exon P1, which contains the start codon, is a total of 168 bp, *F 114 5'UTR and 54 bp coding. The remaining 196 bp of 5'UTR appears to be a *F single exon ~1.3 kb upstream of P1; I say appears because this is based on *F PCR using primers in the presumed P0 and P1. I have not been able to *F confirm this using genomic sequence -- I can't get hold of an assembled *F contig in the region from GenBank or BDGP. I went to GeneSeen just today *F and once again the sequence in this region does not seem to be publicly available. *F So there's also a possibility that P0 represents more than one exon." *F *F Text of attachment: *F CTAGAATTCAGCGGCCCGCCCGGGCAGGTAATATTAGCTGCTATAACTAA *F AAGAGCTCAGATTCCTTTTTCTTCTTGGTTACCTGCAGCTATAGCTGCTC *F CTACACCTGTTTCTGCTTTAGTTCATTCTTCTACATTAGTTACAGGTGTA *F GGAGCAGGGCTCGTCGTCAGTCTAGAGAGTAATATCGACACGAAAAGAAA *F TTATACCGAGTCGGGTGACCGAGGGAAATCCCAGAACAATTGAATTCAAA *F CGCCGCTGCGATATAAATTCCGATTTGGAGGCGCTGCGGTCGTTGCGTTA *F ACCAAACGAAATG # *U FBrf0136330 *a Richards *b S. *t 2001.7.05 *T personal communication to FlyBase *u *F The following information was provided by Dr. Stephen Richards in response to a curator *F query, regarding the poe[1] and poe[2] alleles: *F > >Both mutations are nonsense mutations, at codons 728 and 883, *F > >respectively. # *U FBrf0136331 *a Pinto *b S. *t 2001.7.06 *T personal communication to FlyBase *u *F The following information about the latheo gene and alleles described in FBrf0109005 *F were provided by Dr. Shirly Pinto on July 6, 2001: *F *F 1) Based on our sequence analysis in combination with the Berkeley genome *F project the correct transcript unit map of latheo is as described in the *F attached document. [FlyBase curator comment: copy of transcript unit *F map is archived.] *F *F 2) The coordinates described in table 1 [of FBrf0109005] correspond to *F AF153209 sequence until nucleotide 2885, but are off by 1 nucleotide *F after that. *F *F 3) In lat[vr6R6], nucleotides CCAGATCC are missing. # *U FBrf0136669 *a Andrew *b D. *t 2001.5.22 *T personal communication to FlyBase *u *F Date: Tue, 22 May 2001 09:10:59 \-0400 *F From: Debbie Andrew *F Subject: RE: FlyBase query (cy1131,1132) *F To: Chihiro Yamada *F Reply to: RE: FlyBase query (cy1131,1132) *F Dear Dr. Yamada, *F 1959 is a placW insert generated in the Scott lab, which I mapped to 67C. *F I don't have records for the embryonic expression pattern, but it gives *F expression in the imaginal discs, including the most distal cells of the *F leg imaginal discs, the notal portion of the wing imaginal disc and the eye *F portion of the eye-antennal disc. *F I hope this information is helpful. *F Debbie Andrew *F Chihiro Yamada wrote: *F >Dear Drs Scott and Andrew. *F > *F >I am currently curating a paper for FlyBase: *F > *F >Hiller et al. 2001, Genes Dev. 15(8): 1021--1030 *F > *F >In this paper a insertion line P1959 is mentioned. Following the *F >reference given for this line, *F > *F >Lin et al., 1996, Development 122(4): 1331--1341 *F > *F >I find the line: *F > *F >'The w+ bearing P-element insert Is(3)1959, was generated in the *F >laboratory of M.Scott and localised cytologically by D. Andrew' *F > *F >Can either of you tell me which transposon was used to generate P1959? *F >Is the insertion a P{lacW}? Do you have any other information about *F >this line? *F > *F >Any new information you give will be curated as a personal *F >communication from you to FlyBase, if thats fine with you. *F > *F >Thanks for your time. *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- *F > *F > From: Chihiro Yamada *F > Subject: FlyBase query (cy1131,1132) *F > To: dandrew@jhmi.edu, scott@cmgm.stanford.edu *F Deborah J. Andrew *F Dept. of Cell Biology and Anatomy *F The Johns Hopkins University School of Medicine *F 725 N. Wolfe St. *F Baltimore, MD 21205-2196 *F Ph: 410-614-2722 *F FAX: 410-955-4129 *F email: dandrew@jhmi.edu # *U FBrf0136685 *a Desplan *b C. *t 2001.6.14 *T personal communication to FlyBase *u *F Date: Thu, 14 Jun 2001 12:25:17 \-0700 *F To: Chihiro Yamada *F From: Claude Desplan *F Subject: Pan R7 *F Dear Chihiro: *F There is only one construct call Pan-R7, that drives Gal4. The tetanus *F toxin was driven by this construct under UAS control (Pan-R7-Gal4 X UAS-TT). *F Ali Tahayato made the promoter construct in my lab. It consists of the *F proximal part of the rhodopsin 4 promoter attached to the distal part of *F the rh3 promoter. These two promoters are from genes expressed in *F complementary subsets of R7 cells distributed stochastically in the main *F part of the retina. Pan-R7 becomes expressed in all R7 with fairly high *F specificity. *F The exact construct is below: rh3 promoter from \-160 to \-56, attached to *F rh4 promoter \-63- to \+85 *F Please call me if you have any further question. *F 4012df2.jpg *F At 06:39 PM 06/08/2001 \+0100, you wrote: *F Dear Dr Desplan *F I am currently curating a paper for FlyBase: *F Lee et al. 2001 Neuron 30(2): 437--450 *F Lee at al using a PANR7 promoter to drive expression in two constructs *F \- GAL4 and tetanus toxin light chain. They cite you as the source of *F this promoter fragment. Can you give me some details about this *F promoter. What gene was this promoter derived from, and what fragment *F does it comprise of? *F Thanks for your help, *F Chihiro *F Claude Desplan *F Dept. Biology, NYU *F 1009 Main Building *F 100 Washington Square East *F New York NY 10003-6688 *F Office: (212) 998 8218 *F Lab: (212) 998 3966 *F Private FAX: (212) 995 4710 *F e-mail: claude.desplan@nyu.edu (alias for cd38@nyu.edu) *F Web page: http://homepages.nyu.edu/~cd38/ *F [FlyBase curator comment: e-mail also contained a jpeg image, sent as an *F attachment. It is a figure describing the promoter, with co-ordinates as *F above, and showing restriction enzyme sites \- BamHI at the \-160 end of the *F rh3 fragment, SpeI in between the rh3 and rh4 fragments and EcoRI at the \+85 *F end of the rh4 fragment. This figure has been archived.] # *U FBrf0136701 *a McLennan *b R. *t 2001.5.22 *T personal communication to FlyBase *u *F From ross_mclennan@hotmail.com Tue May 22 17:11:24 2001 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Tue, 22 May 2001 17:11:24 \+0100 *F X-Originating-IP: 130.209.6.41 *F Reply-To: ross@rkjmclennan.fsnet.co.uk *F From: 'Ross McLennan' *F To: ma11@gen.cam.ac.uk *F Cc: j.a.t.dow@bio.gla.ac.uk *F Bcc: *F Subject: Re: BDGP gene annotation *F Date: Tue, 22 May 2001 17:10:56 \+0100 *F Mime-Version: 1.0 *F Content-Type: multipart/mixed; boundary='----=_NextPart_000_5d99_4bd9_6680' *F X-OriginalArrivalTime: 22 May 2001 16:10:58.0193 (UTC) *F FILETIME=C9734810:01C0E2D9 *F Content-Length: 47787 *F A word document is attached to this message explaining my arguments for the *F annotation of 10 Drosophila genes as members of the syntaxin family involved *F in vesicle fusion. *F Thank you. *F Ross McLennan *F \------------------------------------------------------------ *F Ross McLennan *F Division of Molecular Genetics *F Anderson College *F University of Glasgow *F Glasgow *F G11 6NU *F Scotland *F Tel: 0141 330 5106 *F Email: rmcle001@udcf.gla.ac.uk *F or ross@rkjmclennan.fsnet.co.uk *F _________________________________________________________________________ *F Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com. *F Analysis of the completed Drosophila melanogaster genome sequence has *F revealed that there are putatively 10 syntaxin genes in this species. *F Several of these genes have already been studied in great detail, in *F particular the Drosophila homologue of syntaxin 1A. However, the vast *F majority of this family of genes has not been ananlysed in any great *F detail. *F I suggest the following nomenclature for the members of the Drosophila *F syntaxin gene family (see table below) based on the homology of the *F predicted products of the Drosophila genes to proteins which have *F previously been characterised in other species (although the same *F phylogenies are seen at the nucleotide level). The closest homolgues *F of each of the Drosophila syntaxin gene products (using the BLASTp *F 2.1.1 programme at http://www.ncbi.nlm.nih.gov/blast/blast.cgi) were *F found to be mammalian syntaxin proteins (see attached table and *F phylogenetic tree) and I suggest that the Drosophila genes be named *F corresponding to their closest mammalian orthologue. *F In particular, I question the naming of Drosophila Syx7 and Syx6/10 as *F this does not agree with the nomenclature system described above. In *F reality the closest homologue of CG7736 (currently named syx7) is human *F syntaxin6, whist the closest homologue of CG5081 (currently named *F Syx6/10) is rat syntaxin 7. *F \---------------------------------------------------------------------------- *F Drosophila gene | Current Drosophila | Closest orthologue | Proposed | *F accession number | gene name | (species) | Drosophila | *F | (Flybase 21.05.2001) | | gene name | *F \---------------------------------------------------------------------------- *F CG5448 | Syx1A | Syntaxin 1A (rat) | DmSyx1A | *F \---------------------------------------------------------------------------- *F CG2715 | EG:95B7.1 | Syntaxin 4 (mouse) | DmSyx4 | *F \---------------------------------------------------------------------------- *F CG4214 | Sed5 | Syntaxin 5 (human) | DmSyx5 | *F \---------------------------------------------------------------------------- *F CG7736 | Syx7 | Syntaxin 6 (human) | DmSyx6 | *F \---------------------------------------------------------------------------- *F CG5081 | Syx6/10 | Syntaxin 7 (rat) | DmSyx7 | *F \---------------------------------------------------------------------------- *F CG4109 | \- | Syntaxin 8 (human) | DmSyx8 | *F \---------------------------------------------------------------------------- *F CG11278 | \- | Syntaxin 13 (rat) | DmSyx13 | *F \---------------------------------------------------------------------------- *F CG1467 | Syx16 | Syntaxin 16 (human) | DmSyx16 | *F \---------------------------------------------------------------------------- *F CG7452 | \- | Syntaxin 17 (rat) | DmSyx17 | *F \---------------------------------------------------------------------------- *F CG13626 | \- | Syntaxin 18 (human) | DmSyx18 | *F \---------------------------------------------------------------------------- *F FlyBase curator comment: copy of phylogenetic tree is archived # *U FBrf0136706 *a Roote *b J. *t 2001.5.23 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Wed May 23 12:32:18 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 23 May 2001 12:32:18 \+0100 *F To: j.roote@gen.cam.ac.uk *F Subject: question about l(2)34Df *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 23 May 2001 12:32:19 \+0100 *F Content-Length: 325 *F Hi John, *F I am curating a paper (Pflumm et al, 2001, DEvelopment 128(9): *F 1697--1707) in which they use the allele 'l(2)34DfL480' which they *F got from you. *F Is this the same allele as the one we have in our files called *F 'l(2)34Df2' (synonym of Orc52), which also has the synonym '64-480' *F or is it a new allele ? *F Gillian *F From j.roote@gen.cam.ac.uk Wed May 23 12:40:25 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 23 May 2001 12:40:25 \+0100 *F Mime-Version: 1.0 *F X-Sender: jr32@mole.bio.cam.ac.uk *F Date: Wed, 23 May 2001 12:38:23 \+0100 *F To: Gillian Millburn (Genetics) *F From: John Roote *F Subject: Re: question about l(2)34Df *F >Hi John, *F > *F >I am curating a paper (Pflumm et al, 2001, DEvelopment 128(9): *F >1697--1707) in which they use the allele 'l(2)34DfL480' which they *F >got from you. *F > *F >Is this the same allele as the one we have in our files called *F >'l(2)34Df2' (synonym of Orc52), which also has the synonym '64-480' *F >or is it a new allele ? *F > *F >Gillian *F Hi Gillian, *F L480 = 64-480. L = Lindsley. Same screen as 35BbL692. *F Also l(2)35Bffs720 = 64-720, l(2)35Bbms1638 = 64-1638, *F ms(2)34Fe1715 = 64-1715 .. *F J # *U FBrf0136707 *a Mathog *b D. *t 2001.5.23 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Wed May 23 09:41:21 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 23 May 2001 09:41:21 \+0100 *F To: mathog@caltech.edu *F Subject: Nuria *F Cc: ma11@gen.cam.ac.uk, celniker@bgdp.lbl.gov, gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Wed, 23 May 2001 09:41:20 \+0100 *F Content-Length: 9533 *F David *F You recently communicated a personal comm to FlyBase about the Nuria *F element. We think that it may be the same as the Tinker element *F recently published by Frame et al., 2001, Gene 263(1-2): 219--230. *F The paper did not give the sequence in any usable way but *F we obtained the data from the authors and have included it in the *F FlyBase transposable element sequence set. *F You can get the file from *F http://www.fruitfly.org/sequence/dlMisc.shtml *F as *F na.te.dros or na.te.dros.Z *F and search against it in the BDGP Blast server. *F Let us know please, tho it will be a pity to loose Nuria as a name \! *F Michael *F \--- *F For your convenience I include it here in embl format: *F ID Tinker standard; DNA; INV; 6112 BP. *F XX *F AC nnnnnnnn; *F XX *F DR FLYBASE; ; Tinker. *F XX *F FT source complement(AC004377:71947..77835) *F FT repeat_unit 1..224 *F FT /repeat_type='5' LTR' *F FT repeat_unit 5889..6112 *F FT /repeat_type='3' LTR' *F FT CDS 577..5820 *F XX *F CC Michael Ashburner, 5-May-2001. *F CC Any changes to original sequence record are annotated in an FT line. *F CC Sequence coordinates from M. Butler, Personal communication to *F CC FlyBase, 30 April 2001. *F XX *F SQ Sequence 6112 BP; 1853 A; 1502 C; 1247 G; 1510 T; 0 other; *F TGTACGGATT ATGAGCGGAG TCACCCGGTG CTTCAGCTGT CTTAACAGCG GATTAACATT 60 *F TTATATATCG ATGCTAATTG AACTGAACTG TATTCTTTCT TTCGCTCTTG TAATTTTGCG 120 *F GTCATAGCGA TCAGACGTGA TTTTTGTATA TGAAGAAATA AATGAAGTTA AATAGTGTAT 180 *F ATATGATTCT TATTTGCGAA CCCCATTACA ATGATGTCAA AGCAGTGAGG CATCACAACT 240 *F ATTCATTGGT GGCCCATGAG GGGAACCTTG CTCATAAGAA TCATAATTAA TAATTGCTAA 300 *F AAGCTGTTTG GCAATTTTAT CTTCGTGGTC GGCTTCATCC AGATAAATCA CCGTGCCTAC 360 *F GTTTTCTATC GCGCTCGCTC TCATCACGAC AGAGCAGTAT ACGCAAACAT TCGCGCTCGC 420 *F TCTCATCACG ACAGAGCTAA GTCCCGTTGC CCTTCGATTC CGTTAGATTT GCCGGAATCT 480 *F CTGCCAACTC AAAAAAACAA GACGTCAAAG AAGAACACCG ATAACATACG AGCATTTGTG 540 *F CATATTACAT ACAGTGGAGC AACGTCAGTA TAAACAATGT CGAAATTTGA TCAACTGGTG 600 *F CGAGTTCAAA CTGACCGCAT TGAGTCTTTG AAGCGCTTGT TCAGTAATGT GAAAAAAGAC 660 *F TCGAGTGCGC GGAAAACGGA AATATATTTC GAAAAACGTT TGAATCAAAT CGACGAGTTT 720 *F AACAAAGAAT TTCATCAGGC GCATCACACA CTTATATGCA TGGCAGACTA CGAAGAAAGT 780 *F GTGTATAAGC AACAAAATAC AATCAGCCAA TTCGAAGACC TGGGCATGGA AGTTTACTGT 840 *F TTCGTGGCCG AAGAAAAGAA ACGAATTTAT CCAGGCACAG TGACGCACAA CGAATCAGCT 900 *F AACTCTACAA TAACAACACA TGTAGAGGAA GTACGGATAC CACTGCCAAA ATTACCAGTT 960 *F CCGAAATTCT CTGGCAACTG CGCGGACTGG CCAAGTTTTC ATGATGCATT TTTACGCTTA 1020 *F ATTCACAATA ATGAGCGTCT GGATAAGATT CAAAGGTTCC ATTTTTTAAA GGAAGCATTA 1080 *F CCAGTAGGCC TGGACAACGA CATTCGCCAA ATCGCTTTAA CTGAAGCAAA CTATGAAGTC 1140 *F GCTTGGACCA CACTTCTACA ACGATATAAC AACCCACGAA TTGTGTTCGC CAGCCACATG 1200 *F AACATGCTCT ACAACTTACC GAATCTTTCG AAAGAAAAAT CTGCTGACAT ACGGTCTATG 1260 *F GTTAGTACAG TCAACGTCTG CATTGCCGCT TGCAATACCG TCAAGGCGCC ACTACAGGGA 1320 *F GGAGATTTTT GGTTGACTCA TTATCTAACA ACCAAACTAC CCAAAGACAC TCACACAGCT 1380 *F TGGGAGCATC ATCTGGGCAG CAAGATTGAC GTTCCTTCAT ACAAAGATCT GCAACAGTTC 1440 *F CTCAATGATC GACTTGTTAC GTTAGACGCT ATTGAAAGCC GTAACGCGTG CAGCGGCATG 1500 *F AAACAGTCAA ATGAAACTTC AGACGGTACT AAACGCGTGC GTGTGCACAG CGCCCACACC 1560 *F AGGTCGGGTG CTTCCGCGTC CGCTTGCTAT CATTGTGGCA ACTTACACAT ACTTCGAAGA 1620 *F TGTCCGCAAT TTCTTTCAAT GGATTGCTAC CAACGGAAGG AAGTGGCCAG CAAGGCAAAA 1680 *F TTGTGTCTCA ATTGCCTGGG AAAATCGCAC ACACAAGCAA GCTGCCCCAG CAACAAGAAT 1740 *F TGTCTTCATT GTGGTCAACG TCATCACACG ATGTTACATT TTCCAGCAAC ACAGCCAACG 1800 *F CTGATACCCT CCTCGACATC ATGCCAAAGT TCAGCAGCCA GTTCAGATGC CAAGCCGACT 1860 *F CTACAGTGCA TGTCAACTAC AACTTCATCT ATGACTCATC GAAAGGTACT ACTAGCAACA 1920 *F GCTCGGGTTG TACTCAGTAA CACACAGACA GGATGCCAGG CCACGGTAAA CGCGCTTCTT 1980 *F GACCAAGGAT CGGAAGCAAC TATCATTTCG GAGCATGCTG TACAGTCTCT CCAGCTCTCC 2040 *F CGAAGCACAA CTCGCACTGC AATCACCGGA GTCGGTCAAG ATTCAGGACG ACGCTGCAAA 2100 *F TTCATCGTAA GTTGTTCGGT GCAAACAGCA ACTAACCCAA ATTTCTCGTT AAAGGTCGAC 2160 *F GATGCTTATG TTTTGAACAC GTTGACATCA CACATGCCAA GTCAAAGTTT TCCAGCAGGA 2220 *F AACTGGAGTC ATATCCATGG TCTCATGCTC GCAGACCCCT ATTATTATAG ATCCAAGCGA 2280 *F ATCGATATCA TTTTTGGAGC CGACCTTATG GCACAACTCT TGTTACCTGG AACTAAGATT 2340 *F GGCTTGCCAA ACGAACCCAT AGCGCAGAAT ACTCAACTCG GATGGGTTCT GTTAGGCAAT 2400 *F GTTGGCAACA CGCATATTAC ACGCCACATT CGATGTAATC ATGCCATAAT AAACTCCGAA 2460 *F GAGCTGCTTA AGGTATTTTG CGAGGTAGAA TCAGTTCCAG AACGCCCAAA GCTCTCCAAA 2520 *F GAAGATCAAT GGTGCGAGTC TTTCTTTAAG CAGACCCATC AACGTCAACC AGACGGCAGT 2580 *F TATCAAGTCC GTTTGCCATT CAAGAGGAAC TTTGACCCCA GTATGACGCT CGGAAAATCT 2640 *F CATCAGATCG CCCTGAACAG ATATCTTCAA CTCGAAAGGC GCCTTCAAAG GGACCCAGAC 2700 *F AAATGGATCA GATACTGCAA AGGAATTGAA GAATATTTTC AACTAGGTCA AATCACGTTG 2760 *F GCAGAGACGA GCGAAAACTC AACCATAACC ACGGATTCCT ACGGTCGGCA TGTTGCATCA 2820 *F TGCGTGCTAC CACATCATGC AGTTTTCAAA GAAGAAAGCC TCACCACAAA ACAACGTATC 2880 *F GTTTTTGACG CATCGGCCCG GACCTCAAAT GGCAGATCGT TAAACGATGT ACTATGTGTA 2940 *F GGTCCCACGC TGCAAAATGA TCTGCCAGCC GTTCTCTTGA ATTGGAGACA ATATCAGTTT 3000 *F GTTTTCACTG CCGATATACA ACGAATGTAT CGCTGTATCA ATGTCCACCC TGATGACACG 3060 *F CAGTACCAGA GGATTTTATG GCGGGCGGCG GATGGAGTCA TCAAACAGCA TTGCTTAACT 3120 *F ACCGTCACGT TTGGAACAGC GTCTGCACCT TATACAGCTA TAAGAGTCAT CCATCAAATA 3180 *F GCTGAAGACA CACAGACAAA ATATCCTATG GCATCCAACG TTCTCAAAAA TGGGATATAT 3240 *F GTCGACGACA TTCTCTCAGG CGAGCATTCA CAAGAGGCAG CAATACGGAA AAGTTTGCAA 3300 *F ACTATGCTAG CTTTAAAATC CTCTGGCATG GAGCTACGAA AATGGGCTAG TAATGACCAG 3360 *F GATCTGATGG CAACGATACC TCTCGAGCAT CGATGCAAGC AGACATCCCT CAGTTGGGAC 3420 *F AATGCGGACA CCATCAAAAC ACTGGGTATG TACTGGTTGC CCAAGCAAGA TTGCTTCACT 3480 *F TATAAATTAC TAGCAAATAC TCCAGCCGGT ATAACAAAAC GAGAAATCCT ATCGACCATA 3540 *F GCACGTTTAT TTGATCCTCT TGGATTAATC GCTCCAGTTG TAATTTCAGC AAAGATTATA 3600 *F TTGAAAGAAA TCACACTAGC GAAGCAATAT CGCGAGGACG GATCGAGTAC CTCACTGGAT 3660 *F TGGGATGAGC CAGTTCCCAA CACCATTGCC GTCAAATGGC AACAATTTCG ACAGCAACTA 3720 *F ATGAAGGTTA AGACGATCAA AATACCACGC AGCGTCAAAT TTACGCCACT ATTTAGTAGT 3780 *F GAGATACAAC TGCACACCTT TTGCGATGGA TCCTCCAGTG CTTACGCAGC AGCAGTGTAT 3840 *F GCACGCACCC AACAGTCTGA TGGGACTTTC TATACAACGC TCATCGTCGC AAAATCAAAA 3900 *F ATTTCGCCAA CCAAGCCGTT AACAATACCG CGCACTGAGC TATGCGGTGC AGTATTAGCT 3960 *F ACCAAACTCA CCAAATGGGT GCTGGAGAAT AACCGATGGA CCAATGCACA TATATCTACC 4020 *F TTTTACTGGA CCGATGCTAC CATTGTTCTG CACTGGATTA AAGGAGACAT TACTAGGTGG 4080 *F AAAACGTTTG TAGCCAACCG AGTGTCTTAC ATTCTCGACC ACACCTCAGC GGCTCAGTGG 4140 *F CACCACATAG ACACATCGGA AAACCCAGCA GATTGCGCAA CCAGAGGCTT ACCACCGAGC 4200 *F CAAATACCTG ATATTTGGTG GCATGGCCCA TCCTGGCTAT GCAAACCACA CAATATTTGG 4260 *F CCAAACACGC AATCACAATT GCTCAATCCA GAAGAACGGG ATCTGGAGGC CAAATCCATA 4320 *F AAAATTAGAG CCTTCACTAC CTTGTCAGAC ACAAAGGATT CTATTATTGA CCGATTCTCG 4380 *F TCGTATACAA AACTGCTACG TGTCACGGCA TACATGTTAC GATTCTGCCA CAATGCTCAT 4440 *F GCTCGAGCAC AACGAAGTCA CGGATCACTC TCTCCTGACG AGCTGGATGA GGCCCTGTGC 4500 *F TGCATAGCTC GCCTTGCACA ATCCGATACA TTTCACGCCG ACATCCAAGC GCTTAAAAGG 4560 *F AACAAGCCGT TACCACCCCG TAGCACACTT TCAAATCTTA CACCGTTTCT TGACAACAAT 4620 *F ATCTTGAGGA TTCGTGGCCG TCTCAAGCAT TCAAATCTCT CTTTTTCTCG AAAACATCCA 4680 *F ATTATATTAC CTCATTGTCA CCTATTTACA GATTTGGTAA TTCAACACTC TCATCAGCTC 4740 *F ACTCTACATG GCGGCGCTCA ATTAACACTG GCTCACATAC GCTACAAATT CTGGATTCCC 4800 *F AGAGGCAGAC AAGCAGTCAG GCGAATCATC CGGAAATGCG TCACATGTTT TAAGGTAGCT 4860 *F CCAGTCGTAG CGAAGCAATT GATGGGTGAC TTGCCATTAC ATCGAGTCAA CCCCCCAACT 4920 *F CGTCCGTTCA TTACAACAGG AGTTGACTAC ACTGGTGCGA TTGAACTTCA AGCCGCGCGT 4980 *F GTACGAGGAT CAACCACCTA CAAAGGCTAT GTAGCCATTT TTATATGCTT AGCAACCAAG 5040 *F GCGGTCCACT TGGAAGCCGT CACTGGACTT TCAACAGAGC ACTTCCTGCA AGCATTTACG 5100 *F CGATTCACCG GACGTCGAGG ACAAGTTCAA CATATGTATA GTGATAACGG CACAAATTTT 5160 *F GTTGGCGCGA GTACATCGCT TAACCAGCCC ATCACTTGCA AGGCAGCCCT AAACGAATCG 5220 *F ACATGTCAAC ATGGGACAAG GTGGCATTTC ACACCTCCAT ATTCTCCCAA TTTTGGTGGC 5280 *F ATCTGGGAGG CAAACGTGAA AGCAATGAAG CATCATCTTA AACGGATCGT CGGCAGCCAC 5340 *F AAGCAGACGT ATGAGGAGCT TACTACAGTT TTGATCAGGA TTGAAGCATG TTTGAACTCA 5400 *F CGTCCACTTT GCCCGCTAAC CGCCGACCCT GACGATTTAG AAGTACTAAC TCCAGCACAT 5460 *F TTCTTAATTG GTGACGCACT ACTGGCACCG CCACAAGGTC GGCCGAATAA TAAGCCTTTG 5520 *F CGTGAACTAT TTCTCGCACA ACAACACATG ACGCGACAAT TCTGGTCTCA ATGGTCTCGT 5580 *F GATTGGTTAT CACACTTGCA AACTCGGCCA AAGTGGTGCC AAATCAAAGA TAATCTTAGC 5640 *F ATCAACGACT TAGTCATTAT AAAGGATGAT AATCTACCAC CAGCTAAATG GACTATAGGC 5700 *F CGAGTCGTCG AACTGCACCC TGGATCGGAC TCGCTAGTCA GAGTGGTTAC ATTAAAGACG 5760 *F AAATCTGGCA TTCAAAAGAG GTCAATCACA AAGCTTTGTC CACTTCCGAT TTCAACATAA 5820 *F TTATGATCAA CACACGGATC AAGAATCACA AGGAGCCTTC ATGCTGGACG ACGCATTGGC 5880 *F GGGCGGCATG TACGGATTAT GAGCGGAGTC ACCCGGTGCT TCAGCTGTCT TAACAGCGGA 5940 *F TTAACATTTT ATATATCGAT GCTAATTGAA CTGAACTGTA TTCTTTCTTT CGCTCTTGTA 6000 *F ATTTTGCGGT CATAGCGATC AGACGTGATT TTTGTATATG AAGAAATAAA TGAAGTTAAA 6060 *F TAGTGTATAT ATGATTCTTA TTTGCGAACC CCATTACAAT GATGTCAAAG CA 6112 *F // *F From MATHOG@seqaxp.bio.caltech.edu Wed May 23 17:01:11 2001 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 23 May 2001 17:01:11 \+0100 *F Date: Wed, 23 May 2001 9:01:11 \-0700 *F From: David Mathog *F To: ma11@gen.cam.ac.uk *F Subject: RE: Nuria *F Content-Length: 1673 *F >You recently communicated a personal comm to FlyBase about the Nuria *F >element. We think that it may be the same as the Tinker element *F >recently published by Frame et al., 2001, Gene 263(1-2): 219--230. *F I compared the sequence you sent with mine and they are the same element. *F They found it before me, even if they didn't provide the sequence, so *F Tinker it is. (Funny though that they only noted it in AC004377 when it's *F present in many more locations.) *F > *F >The paper did not give the sequence in any usable way but *F >we obtained the data from the authors and have included it in the *F >FlyBase transposable element sequence set. *F Ok. *F I pointed it out because it wasn't annotated in any of the *F locations where I found it. That included (and started with) Celera *F sequence AE003714 bases:188413-194132 (193592-194132 corresponds to Tinker *F 530-1,188413-193591 is all Ns, which are presumably more of Tinker). *F This is a region of the BXC (between bxd and glut) which had been sequenced *F previously (DMU31961) by the same people (Celniker) using the same strain *F (y; cn bw sp), and there was no Tinker there. Tinker also appears on the *F ends of many of the Celera fragments, which makes sense since the *F automatic assembly method will break down on a repeated element of that *F size. *F Anyway, I have not looked through the other mobile elements to see if *F anything else was hopping around, but if the stock was the one it is *F claimed to be in both cases then this element was apparently moving quite *F recently in the stock that was used for Celera's sequencing project. *F Regards, *F David Mathog *F mathog@caltech.edu *F Manager, sequence analysis facility, biology division, Caltech # *U FBrf0136708 *a Park *b Y. *t 2001.5.22 *T personal communication to FlyBase *u FlyBase error report for CG15520 on Tue May 22 18:48:04 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed May 23 03:24:54 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 23 May 2001 03:24:54 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 22 May 2001 18:48:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ypark@ucrac1.ucr.edu *F Subject: FlyBase error report for CG15520 on Tue May 22 18:48:04 2001 *F Content-Length: 1002 *F Error report from Yoonseong Park (ypark@ucrac1.ucr.edu) *F Gene or accession: CG15520 *F Release: 1 *F Gene annotation error *F Gene CG15520 corresponds to FBgn0015520 *F Protein sequence: *F MKSMLVHIVLVIFIIAEFSTAETDHDKNRRGANMGLYAFPRVGRSDPSLANSLRDGLEAGVLDGIYGDASQEDYNEADF *F QKKASGLVAFPRVGRGDAELRKWAHLLALQQVLDKRTGPSASSGLWFGPRLGKRSVDAKSFADISKGQKELN *F Comments: According to the GH21009.5prime sequence, predicted translation is *F corrected as follows, *F Met as translation initiation is corrected to be @69 bp in GH21009.5prime from *F previous prediction starting @ 168 bp. *F This change still keeps reading frame except longer N terminal sequence as 33 *F additional amino acids. The added N-terminal amino acid sequence appears to *F have signal peptide that is supposed to be in the neuropeptide gene like *F CG15520. The new translation also predicts RR as dibasic cleavage site for *F the first mature peptide as 'GANMGLYAFPRV'. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0136709 *a Corey *b D.P. *t 2001.5.19 *T personal communication to FlyBase *u *F From bdgp-admin@fruitfly.bdgp.berkeley.edu Sat May 19 02:12:31 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 19 May 2001 02:12:31 \+0100 *F X-Sender: corey/helix.mgh.harvard.edu@127.0.0.1 *F X-Mailer: QUALCOMM Windows Eudora Version 5.0.2 *F Date: Fri, 18 May 2001 21:14:09 \-0400 *F To: bdgp@fruitfly.org *F From: 'David P. Corey' *F Mime-Version: 1.0 *F Subject: BDGP annotation *F Sender: bdgp-admin@fruitfly.bdgp.berkeley.edu *F X-BeenThere: bdgp@fruitfly.BDGP.Berkeley.EDU *F X-Mailman-Version: 2.0beta6 *F List-Help: *F List-Post: *F List-Subscribe: , *F *F List-Id: BDGP mailing list *F List-Unsubscribe: , *F *F Content-Type: multipart/mixed; boundary='=_AAB7tQAAOz47CPbn' *F Content-Length: 2525 *F I notice that you have very little annotation for gene EG:84H4.1. *F This encodes a protein that we have called torp4a, which is related to *F TOR1A, the human gene defective in torsion dystonia. It is part of a *F gene family that includes 4 mammalian, 1 zebrafish, and 3 nematode *F members. The human protein appears to form a hexameric structure, *F similar to Hsp101 or NSF. *F A table cataloging these genes, with accession numbers, is in the *F following reference. I can send a copy if it would be useful. *F Ozelius LJ, Page CE, Klein C, Hewett JW, Mineta M, Leung J, Shalish C, *F Bressman SB, de Leon D, Brin MF, Fahn S, Corey DP, Breakefield XO. *F The TOR1A (DYT1) gene family and its role in early onset torsion *F dystonia. *F Genomics. 1999 Dec 15;62(3):377-84. *F _______________________________________________ *F BDGP mailing list *F BDGP@fruitfly.BDGP.Berkeley.EDU *F http://fruitfly.BDGP.Berkeley.EDU/mailman/listinfo/bdgp # *U FBrf0136711 *a Robertson *b H.M. *t 2001.5.20 *T personal communication to FlyBase *u *F From hughrobe@life.uiuc.edu Sun May 20 06:27:58 2001 *F Envelope-to: MA11@gen.cam.ac.uk *F Delivery-date: Sun, 20 May 2001 06:27:58 \+0100 *F Date: Sun, 20 May 2001 00:28:04 \-0500 *F From: hughrobe@life.uiuc.edu *F Subject: Or2a *F To: Ashburner_Michael *F X-Authenticated: *F MIME-Version: 1.0 *F Michael, *F .. The Celera version of *F Or2a is probably correct, with an unusual gc donor splice site for a short *F intron in a highly conserved phase 0 location, and the resulting additional *F exon encodes the second half of TM7 and is alignable with other family *F members. Noone has RT-PCR evidence for this yet, but they do have in situ *F evidence that the gene is transcribed. There are two other Or genes that seem *F to have gc donors as well. *F ATGGAGAAGCAAGAGGATTTCAAACTGAACACCCACAGTGCTGTGTACTACCACTGGCGCGTTTGGGAGCTCACTGGC *F CTGATGCGTCCTCCGGGCGTTTCAAGCCTGCTTTACGTGGTATACTCCATTACGGTCAACTTGGTGGTCACCGTGCTG *F TTTCCCTTGAGCTTGCTGGCCAGGCTGCTGTTCACCACCAACATGGCCGGATTGTGCGAGAACCTGACCATAACTATT *F ACCGATATTGTGGCCAATTTGAAGTTTGCGAATGTGTACATGGTGAGGAAGCAGCTCCATGAGATTCGCTCTCTCCTA *F AGGCTCATGGACGCTAGAGCCCGGCTGGTGGGCGATCCCGAGGAGATTTCTGCCTTGAGGAAGGAAGTGAATATCGCA *F CAGGGCACTTTCCGCACCTTTGCCAGTATTTTCGTATTTGGCACTACTTTGAGTTGCGTCCGCGTGGTCGTTCGCCCG *F GATCGAGAGCTCCTGTATCCGGCCTGGTTCGGCGTTGACTGGATGCACTCCACCAGAAACTATGTGCTCATCAATATC *F TACCAGCTCTTCGGCTTGATAGTGCAGGCTATACAGAACTGCGCTAGTGACTCCTATCCGCCTGCGTTTCTCTGCCTG *F CTCACGGGTCATATGCGTGCTTTGGAGCTGAGGGTGCGGCGGATTGGCTGCAGGACGGAAAAGTCCAATAAAGGGCAG *F ACATATGAAGCCTGGCGGGAGGAGGTGTACCAGGAACTCATCGAGTGCATCCGCGATCTGGCGCGGGTCCATCGGCTG *F AGGGAGATCATTCAGCGGGTCCTTTCAGTGCCCTGCATGGCCCAGTTCGTCTGCTCCGCCGCCGTCCAGTGTACCGTC *F GCCATGCACTTCCTGTACGTAGCGGATGACCACGACCACACCGCCATGATCATCTCGATTGTATTTTTCTCGGCCGTC *F ACCTTGGAGGTGTTTGTAATCTGCTATTTTGGGGACAGGATGCGGACACAGAGCGAGGCGCTGTGCGATGCCTTCTAC *F GATTGCAACTGGATAGAACAGCTGCCCAAGTTCAAGCGCGAACTGCTCTTCACCCTGGCCAGGACGCAGCGGCCTTCT *F CTTATCTACGCAGGCAACTACATCGCACTCTCGCTGGAGACCTTCGAGCAGgcaaggcacaattatatattcaaatgt *F cctttgatttgattcaaatatgatatgattcacttgattccagGTCATGAGGTTCACATACTCTGTTTTCACACTCTT *F GCTGAGGGCCAAGTAA *F .. *F Hugh *F Hugh M. Robertson *F Professor *F Department of Entomology *F University of Illinois at Urbana-Champaign *F 320 Morrill Hall, MC118 *F 505 S. Goodwin *F Urbana, IL 61801 *F Phone 217-333-0489 *F FAX 217-244-3499 *F Email hughrobe@life.uiuc.edu *F www.life.uiuc.edu/robertson/lab.html # *U FBrf0136732 *a Berg *b J. *t 2001.5.30 *T personal communication to FlyBase *u FlyBase error report for CG5501 on Wed May 30 11:13:32 2001. *F From jsberg@med.unc.edu Wed May 30 16:13:47 2001 *F To: ''gm119@gen.cam.ac.uk'' *F Subject: FlyBase paper *F Date: Wed, 30 May 2001 11:13:32 \-0400 *F Dear Gillian- *F I am the graduate student in Richard Cheney's lab who has done most of the *F myosin gene annotation. You recently sent Richard an E-mail about curating *F the Oliver et al. paper regarding the myosin gene at 29C3-D1. This gene is *F indeed the CG10595 transcript. *F I am also attaching our most recent work which stems from the Oliver et al. *F paper, entitled 'A Millennial Myosin Census.' This reference is a more *F comprehensive one which we think identifies the full complement of *F Drosophila myosin genes, including a novel myosin that does not appear to be *F recognized in the FlyBase (95E, AE003746). This myosin was partially *F predicted as CG5501, acc. \# AAF56246 but the Celera prediction lacks most of *F the motor domain and tail domain. I have attached my predicted amino acid *F sequence (Dm95E.txt) and cDNA (Dm95E_cds.txt) for this myosin in FASTA *F format so that you can compare it to FlyBase. I have also attached a Word *F document containing the GenScan output I obtained for AE003746. I did find *F two EST sequences (acc. \# AI405551 and AA698637) that are 99-100% identical *F to the GenScan predicted coding sequence with the exception of several gaps, *F suggesting that the longer transcript is expressed in some form. *F For the sake of completeness, you may also wish to look at a recent paper by *F Yamashita et al. (2000), 'Identification and analysis of the myosin *F superfamily in Drosophila: a database approach.' J. Muscle Res. Cell Motil. *F 21, 491-505, which takes a similar approach as ours and obtains essentially *F the same list of genes. *F Thank you for your efforts in annotating the Fly myosins, and please let me *F know if you have any other questions. *F Sincerely, *F Jonathan Berg *F Department of Cell and Molecular Physiology *F The University of North Carolina at Chapel Hill *F \------------------------------------------------------------------------------- *F >Dm95E AE003746|GENSCAN_predicted_CDS_26|3249_bp *F atggagcaggaaatcggcacctgggactcggtactgttggagaacctgtccgaggatagt *F ttcataaacaacatccaccagcgctataagcgcgatcacatatatacctacattggaaca *F tctgttgtggctctgaatccatatcatcacatatccgagcactctctggacaatgtccgc *F aactatggcgataagggcattttccagctgccgccccacatatatggtctcacaaatctg *F gcttatcaatcgctcaaagatcagagcgaggatcagtgtgttctgctcaccggtgagagc *F ggagcgggcaaaacggagacttttaaaatgatcgtgaactttctgacccacatacaagat *F cgctcccactgccccccaacaccgaatgttttgcgcaagcaatcctcaactagctcggcc *F agcggattggtgatgcacgcccacaggcgagcctccagcagctgctccggcactgccaat *F tttattatatgcaaaaaccgggcggaaaatccgtcaggcagtgtttcacggcgacaaagt *F ccatcgccaggaccatcgcagcgatcgcggacgcgggccgagagcatcgagcgccaaagc *F aggcgccacatgcgggagaaaattgtcgactttgatttctcacaccacaagagtagcgaa *F aacatcagcggccttcctgaatcgcacgcccaccacatgcatccgaccaagtcgtgcttc *F aagcaccagcagacccaagtcagcgcctgcactgcaatgcccgcagcagccaagggatcg *F cccaaatacgcggtacccaccgtgtacggcggttgccgccagtgcggacacagcaagtgt *F gtccgtgctcagagcctggaaaaggaggagcgggatgatctacgaggcagcaactgccgc *F ctgtctaccatagccactgccaccaccaatccggcacatccgcatcgcggtagttgctcc *F aacctgatgcgccagcactcgacagagagtcagccggagcgggagcgggatcgaagctcc *F cttatggggtccacgcaacgtatatcgctgtacgatgcacacaagctgagtaaggttctg *F ggcgatctgccgccgcctccgcctagttctgtttcgcccacgccatcggccagctcttcg *F ctgcacaggcgtcataaatcgcccacgcaacgaatgcgagagtgcgtcacctgtgcggat *F gtgttcctggaggccatgggcaatgcctgcaccctgaaaaataataactctagtcgatat *F acccgtataacggaccctatcattggagagcgaaattttcacatcttttaccaattacta *F ttaggagctgatctccagttgctaaaatcgctcaagctgtatcgaaatgtggaaaagtac *F gagctgctccgcaacacaactgccatggaggaggaccgcatgaattttcattatacgaag *F aattttgtgtttcatgtgctgcgttcggagcaagagctctatattcgcgagggattggaa *F tggtctcgcattgactatttcgacaacgagtctatttgcgagttaatagacaaacccagc *F tatggtatattgagcttgattaatgaaccccatttaaatagcaacgacgctttgcttttg *F cgagttcagcaatgttgtgcggggcatcccaactttatgaccaccggcagcaattccatg *F tgctttcagattcgtcattatgcaagtgtagtgaactactcaatacatcggtttctcgaa *F aagaactccgacatgctgccgaagtacataagcgctgccttttatcagagcaaactttct *F ttggtgcaaagcctattccccgaggggaatccccgtcgacaggttaccaaaaagcccagc *F acgttgagttcgaatatccgcacccaattgcagacgctgctggccatcgttaagcatcgc *F cgctcccactatgtgttctgtattaagcccaacgagggcaagcagccgcaccagttcgat *F atggctctagtgcaacatcaggtgcgctacatgtcgctcatgccgctggtccacctgtgt *F cgcactggccattgctaccacctgttgcacgttaagttttttcatcgctataagttgctc *F aacagcctgacgtggccccactttcatggcggcagtcaggtagagggtatcgccctcata *F atccgtaacctaccgctgccctcagcggagttcacgatcggcaccaaaaatgtgttcgtg *F cgtagtccccgcaccgtatatgagttggaacagtttcgccgcctgcgtattagcgagctg *F gccgtgcttattcaaaccatgttccgaatgtatcacgcaaggaagcgctttcagcgcatg *F cgacacagccagatgatcatatcgagtgcctggcgcacgtggcgggcccgcgaggagtat *F cggtccttgaagtacaaacgacaggtgagatgggccatcgatattataggccgctactac *F cgccagtggaagatcagacagttccttctgacaattcccttgcgactgccaccgaacacg *F ctaagcccgctctccaccgaatggccagtggctcccgcatttctggcagatgcctctcgt *F catcttaggtccatataccatcgttggaagtgctacatctaccgaaactcctttgatcaa *F acggcgcgtaatcgaatgcgggagaaggtcacagccagcattatcttcaaggatcgaaaa *F gcttcatatggacgaagtgtgggtcatccttttgtgggggactacgtgcgactgcgacac *F aaccagcagtggaaaaagatctgcgccgagaccaacgatcagtatgttgtattcgcagac *F ataatcaacaagatagcgcgctccagtggcaagtttgtgcccattttgctggtgctatcc *F acgtcatcgcttttgctgttggaccaacgaacgctgcaaattaagtacagagtgcctgca *F tcggagatttaccgaatgtctctgagcccctacctagatgacattgctgtgtttcactct *F gaatttggacggaagaagggtgatttcgtttttcaaacgggtcatgtgattgaaattgtt *F accaaaatgtttctggtcatacaaaatgccacaggcaaacccccggagatacacataagc *F actgaatttgaagcgaacttcggccagcagactgtcatcttttcgttcaaatacggcggc *F atgtcggacttagcacaaggcccacccaaggtcacacgcaaggcgaaccgcatggagata *F attgtgtga *F \------------------------------------------------------------------------------- *F >Dm95E AE003746|GENSCAN_predicted_peptide_26|1082_aa *F MEQEIGTWDSVLLENLSEDSFINNIHQRYKRDHIYTYIGTSVVALNPYHHISEHSLDNVR *F NYGDKGIFQLPPHIYGLTNLAYQSLKDQSEDQCVLLTGESGAGKTETFKMIVNFLTHIQD *F RSHCPPTPNVLRKQSSTSSASGLVMHAHRRASSSCSGTANFIICKNRAENPSGSVSRRQS *F PSPGPSQRSRTRAESIERQSRRHMREKIVDFDFSHHKSSENISGLPESHAHHMHPTKSCF *F KHQQTQVSACTAMPAAAKGSPKYAVPTVYGGCRQCGHSKCVRAQSLEKEERDDLRGSNCR *F LSTIATATTNPAHPHRGSCSNLMRQHSTESQPERERDRSSLMGSTQRISLYDAHKLSKVL *F GDLPPPPPSSVSPTPSASSSLHRRHKSPTQRMRECVTCADVFLEAMGNACTLKNNNSSRY *F TRITDPIIGERNFHIFYQLLLGADLQLLKSLKLYRNVEKYELLRNTTAMEEDRMNFHYTK *F NFVFHVLRSEQELYIREGLEWSRIDYFDNESICELIDKPSYGILSLINEPHLNSNDALLL *F RVQQCCAGHPNFMTTGSNSMCFQIRHYASVVNYSIHRFLEKNSDMLPKYISAAFYQSKLS *F LVQSLFPEGNPRRQVTKKPSTLSSNIRTQLQTLLAIVKHRRSHYVFCIKPNEGKQPHQFD *F MALVQHQVRYMSLMPLVHLCRTGHCYHLLHVKFFHRYKLLNSLTWPHFHGGSQVEGIALI *F IRNLPLPSAEFTIGTKNVFVRSPRTVYELEQFRRLRISELAVLIQTMFRMYHARKRFQRM *F RHSQMIISSAWRTWRAREEYRSLKYKRQVRWAIDIIGRYYRQWKIRQFLLTIPLRLPPNT *F LSPLSTEWPVAPAFLADASRHLRSIYHRWKCYIYRNSFDQTARNRMREKVTASIIFKDRK *F ASYGRSVGHPFVGDYVRLRHNQQWKKICAETNDQYVVFADIINKIARSSGKFVPILLVLS *F TSSLLLLDQRTLQIKYRVPASEIYRMSLSPYLDDIAVFHSEFGRKKGDFVFQTGHVIEIV *F TKMFLVIQNATGKPPEIHISTEFEANFGQQTVIFSFKYGGMSDLAQGPPKVTRKANRMEI *F IV *F \------------------------------------------------------------------------------- *F GENSCAN 1.0 Date run: 2-Aug-100 Time: 15:08:37 *F Sequence AE003746 : 226318 bp : 44.61% C+G : Isochore 2 (43 \- 51 C+G%) *F Parameter matrix: HumanIso.smat *F Predicted genes/exons: *F Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr.. *F \----- \---- \- \------ \------ \---- \-- \-- \---- \---- \----- \----- \------ *F 1.05 PlyA \- 297 292 6 1.05 *F 1.04 Term \- 2142 1618 525 0 0 76 53 461 0.692 35.66 *F 1.03 Intr \- 2571 2470 102 0 0 47 29 117 0.528 2.07 *F 1.02 Intr \- 3211 3058 154 0 1 30 85 217 0.995 15.67 *F 1.01 Init \- 7886 7612 275 2 2 85 113 295 0.827 28.14 *F 1.00 Prom \- 7995 7956 40 \-7.96 *F 2.00 Prom \+ 9051 9090 40 \-8.86 *F 2.01 Init \+ 9315 9453 139 2 1 60 97 253 0.999 23.70 *F 2.02 Intr \+ 9516 9809 294 1 0 60 15 523 0.991 39.18 *F 2.03 Term \+ 9878 11271 1394 0 2 78 45 1678 0.999 153.87 *F 2.04 PlyA \+ 11890 11895 6 1.05 *F 3.03 PlyA \- 12262 12257 6 \-1.75 *F 3.02 Term \- 13936 12728 1209 1 0 61 48 1431 0.908 128.06 *F 3.01 Init \- 14666 14049 618 2 0 63 107 960 0.999 90.75 *F 3.00 Prom \- 14835 14796 40 \-9.36 *F 4.00 Prom \+ 15166 15205 40 \-10.35 *F 4.01 Init \+ 15627 16380 754 2 1 28 113 854 0.999 76.77 *F 4.02 Intr \+ 16713 16883 171 1 0 65 97 248 0.999 23.31 *F 4.03 Intr \+ 17029 17214 186 2 0 46 119 212 0.999 19.76 *F 4.04 Intr \+ 17424 17590 167 1 2 78 59 155 0.961 11.28 *F 4.05 Intr \+ 17686 17938 253 0 1 38 100 181 0.697 11.31 *F 4.06 Term \+ 17997 18742 746 1 2 111 34 971 0.999 87.62 *F 4.07 PlyA \+ 19751 19756 6 1.05 *F 5.06 PlyA \- 20217 20212 6 1.05 *F 5.05 Term \- 21112 20952 161 2 2 15 39 220 0.903 7.90 *F 5.04 Intr \- 21263 21183 81 0 0 60 62 65 0.505 0.71 *F 5.03 Intr \- 21408 21315 94 0 1 109 94 87 0.998 10.94 *F 5.02 Intr \- 22384 21476 909 1 0 69 115 1208 0.692 113.08 *F 5.01 Init \- 22783 22556 228 1 0 97 121 332 0.999 35.77 *F 5.00 Prom \- 23309 23270 40 \-6.56 *F 6.00 Prom \+ 23463 23502 40 \-8.56 *F 6.01 Init \+ 25450 25545 96 0 0 84 79 \-34 0.635 \-4.39 *F 6.02 Term \+ 25604 26206 603 1 0 85 37 959 0.880 85.02 *F 6.03 PlyA \+ 26266 26271 6 1.05 *F 7.03 PlyA \- 26322 26317 6 \-1.95 *F 7.02 Term \- 27584 26533 1052 0 2 33 39 1554 0.998 137.30 *F 7.01 Init \- 27755 27641 115 2 1 68 39 208 0.999 14.29 *F 7.00 Prom \- 28178 28139 40 \-12.21 *F 8.00 Prom \+ 28402 28441 40 \-7.16 *F 8.01 Sngl \+ 29165 30508 1344 1 0 85 48 1759 0.999 167.74 *F 8.02 PlyA \+ 30687 30692 6 1.05 *F 9.00 Prom \+ 31440 31479 40 \-7.86 *F 9.01 Sngl \+ 31636 32079 444 0 0 61 36 516 0.385 39.94 *F 9.02 PlyA \+ 32182 32187 6 1.05 *F 10.02 PlyA \- 32228 32223 6 1.05 *F 10.01 Sngl \- 34224 32317 1908 0 0 89 31 3523 0.999 340.61 *F 10.00 Prom \- 34464 34425 40 \-1.26 *F 11.00 Prom \+ 35001 35040 40 \-8.76 *F 11.01 Init \+ 35521 35919 399 0 0 32 75 866 0.999 76.37 *F 11.02 Intr \+ 35975 36123 149 1 2 28 66 135 0.998 4.33 *F 11.03 Intr \+ 36182 36350 169 2 1 63 12 324 0.998 22.25 *F 11.04 Intr \+ 36409 37431 1023 0 0 60 94 1582 0.999 146.81 *F 11.05 Intr \+ 37495 38064 570 0 0 56 64 751 0.999 62.46 *F 11.06 Intr \+ 38131 38386 256 0 1 91 106 378 0.999 36.92 *F 11.07 Intr \+ 38443 38747 305 2 2 53 106 388 0.999 33.41 *F 11.08 Intr \+ 38811 38911 101 2 2 56 92 65 0.999 2.61 *F 11.09 Term \+ 38977 39739 763 1 1 54 38 718 0.844 56.29 *F 11.10 PlyA \+ 39910 39915 6 1.05 *F 12.04 PlyA \- 40060 40055 6 1.05 *F 12.03 Term \- 41180 41099 82 1 1 60 43 55 0.200 \-4.63 *F 12.02 Intr \- 41450 41302 149 2 2 123 101 41 0.267 7.93 *F 12.01 Init \- 49043 48981 63 2 0 51 29 208 0.746 12.35 *F 12.00 Prom \- 56701 56662 40 \-1.26 *F 13.00 Prom \+ 57307 57346 40 \-4.46 *F 13.01 Init \+ 63107 63182 76 1 1 45 38 95 0.181 1.45 *F 13.02 Term \+ 72419 72783 365 0 2 26 43 200 0.111 4.03 *F 13.03 PlyA \+ 72843 72848 6 1.05 *F 14.03 PlyA \- 73068 73063 6 1.05 *F 14.02 Term \- 81562 80687 876 1 0 \-33 35 1881 0.010 163.28 *F 14.01 Init \- 84420 83944 477 0 0 39 117 764 0.462 69.82 *F 14.00 Prom \- 84472 84433 40 \-16.42 *F 15.00 Prom \+ 84522 84561 40 \-16.39 *F 15.01 Init \+ 84568 85404 837 0 0 71 64 619 0.181 52.50 *F 15.02 Intr \+ 85888 85933 46 0 1 22 57 68 0.268 \-4.92 *F 15.03 Intr \+ 86396 86707 312 0 0 35 55 345 0.366 22.06 *F 15.04 Term \+ 86814 87016 203 1 2 \-8 37 389 0.739 21.75 *F 15.05 PlyA \+ 87071 87076 6 \-1.75 *F 16.04 PlyA \- 87093 87088 6 \-1.75 *F 16.03 Term \- 87393 87160 234 0 0 13 37 217 0.850 5.42 *F 16.02 Intr \- 87667 87452 216 1 0 66 \-3 181 0.807 5.50 *F 16.01 Init \- 89441 87882 1560 2 0 81 100 1617 0.997 154.67 *F 16.00 Prom \- 90388 90349 40 \-6.66 *F 17.02 PlyA \- 90403 90398 6 1.05 *F 17.01 Sngl \- 93196 91556 1641 1 0 69 47 1661 0.999 154.09 *F 17.00 Prom \- 93309 93270 40 \-7.06 *F 18.00 Prom \+ 93895 93934 40 \-10.64 *F 18.01 Init \+ 93995 94162 168 1 0 36 92 141 0.977 8.86 *F 18.02 Intr \+ 97423 97935 513 0 0 92 105 640 0.969 59.26 *F 18.03 Intr \+ 98166 99446 1281 2 0 73 115 2304 0.666 218.90 *F 18.04 Intr \+ 99997 100148 152 0 2 46 52 184 0.991 9.56 *F 18.05 Intr \+ 100205 101581 1377 2 0 52 89 2177 0.999 202.21 *F 18.06 Intr \+ 101657 101832 176 2 2 22 96 312 0.999 24.98 *F 18.07 Intr \+ 101900 103887 1988 0 2 44 97 3060 0.999 289.83 *F 18.08 Intr \+ 104048 104467 420 1 0 97 77 598 0.995 53.74 *F 18.09 Term \+ 104528 104707 180 1 0 64 44 163 0.899 7.11 *F 18.10 PlyA \+ 104736 104741 6 \-3.84 *F 19.07 PlyA \- 104899 104894 6 1.05 *F 19.06 Term \- 105559 105332 228 1 0 67 48 311 0.981 21.63 *F 19.05 Intr \- 106436 106012 425 0 2 60 62 416 0.978 29.79 *F 19.04 Intr \- 106807 106492 316 1 1 74 113 258 0.998 22.54 *F 19.03 Intr \- 106985 106872 114 2 0 85 58 138 0.996 11.14 *F 19.02 Intr \- 107188 107044 145 0 1 58 44 76 0.972 0.48 *F 19.01 Init \- 107399 107242 158 2 2 13 86 95 0.432 1.18 *F 19.00 Prom \- 107494 107455 40 \-16.52 *F 20.00 Prom \+ 107529 107568 40 \-8.96 *F 20.01 Init \+ 107786 108196 411 1 0 102 113 529 0.695 53.31 *F 20.02 Intr \+ 108258 110568 2311 2 1 71 13 2571 0.999 235.24 *F 20.03 Intr \+ 110626 110783 158 2 2 18 97 348 0.999 28.43 *F 20.04 Term \+ 110910 111449 540 2 0 80 39 776 0.877 66.16 *F 20.05 PlyA \+ 111601 111606 6 1.05 *F 21.00 Prom \+ 111769 111808 40 \-7.06 *F 21.01 Init \+ 112049 112066 18 1 0 52 80 24 0.879 \-1.98 *F 21.02 Intr \+ 112127 113031 905 1 2 53 101 1291 0.999 116.99 *F 21.03 Intr \+ 113101 113688 588 1 0 34 87 694 0.999 55.64 *F 21.04 Intr \+ 113747 113936 190 2 1 31 95 188 0.909 13.39 *F 21.05 Intr \+ 114006 114323 318 2 0 111 119 336 0.999 35.15 *F 21.06 Intr \+ 114396 114480 85 2 1 52 29 127 0.725 2.59 *F 21.07 Intr \+ 116899 117006 108 2 0 104 80 208 0.993 21.86 *F 21.08 Intr \+ 117647 117816 170 0 2 71 103 146 0.986 14.07 *F 21.09 Intr \+ 118626 118780 155 2 2 27 84 102 0.413 2.57 *F 21.10 Intr \+ 122313 122990 678 0 0 66 39 422 0.124 25.83 *F 21.11 Intr \+ 123050 123168 119 2 2 62 92 114 0.985 9.31 *F 21.12 Intr \+ 123229 123422 194 2 2 78 95 274 0.987 26.31 *F 21.13 Intr \+ 123486 123859 374 2 2 \-1 15 465 0.963 24.16 *F 21.14 Intr \+ 123921 124106 186 0 0 68 24 220 0.463 12.40 *F 21.15 Term \+ 124384 124492 109 1 1 39 38 157 0.446 3.78 *F 21.16 PlyA \+ 124546 124551 6 \-3.24 *F 22.02 PlyA \- 124576 124571 6 \-5.80 *F 22.01 Sngl \- 126021 124627 1395 0 0 33 42 1378 0.967 123.76 *F 22.00 Prom \- 126112 126073 40 \-9.06 *F 23.02 PlyA \- 126205 126200 6 1.05 *F 23.01 Sngl \- 127302 126232 1071 0 0 72 38 635 0.985 54.04 *F 23.00 Prom \- 127396 127357 40 \-11.43 *F 24.02 PlyA \- 127573 127568 6 \-0.45 *F 24.01 Sngl \- 128822 127722 1101 2 0 45 47 1255 0.999 114.05 *F 24.00 Prom \- 128902 128863 40 \-11.92 *F 25.00 Prom \+ 129176 129215 40 \-7.46 *F 25.01 Init \+ 130217 130887 671 1 2 68 101 972 0.977 91.18 *F 25.02 Term \+ 130944 131907 964 0 1 46 49 1487 0.999 132.13 *F 25.03 PlyA \+ 132062 132067 6 1.05 *F 26.13 PlyA \- 132299 132294 6 1.05 *F 26.12 Term \- 133259 133136 124 1 1 71 42 190 0.999 10.56 *F 26.11 Intr \- 133492 133365 128 1 2 59 52 86 0.995 1.58 *F 26.10 Intr \- 133706 133563 144 2 0 50 50 97 0.780 2.58 *F 26.09 Intr \- 133902 133767 136 2 1 59 121 168 0.999 17.77 *F 26.08 Intr \- 134295 133964 332 0 2 87 87 414 0.999 35.63 *F 26.07 Intr \- 135207 134511 697 2 1 112 102 597 0.985 54.99 *F 26.06 Intr \- 135514 135267 248 1 2 90 36 151 0.919 6.36 *F 26.05 Intr \- 136303 136209 95 2 2 80 72 234 0.999 20.68 *F 26.04 Intr \- 136446 136362 85 0 1 85 60 41 0.076 0.39 *F 26.03 Intr \- 137806 136768 1039 0 1 47 92 906 0.998 77.40 *F 26.02 Intr \- 137982 137867 116 0 2 72 66 134 0.759 8.85 *F 26.01 Init \- 138150 138046 105 0 0 89 73 101 0.758 8.92 *F 26.00 Prom \- 138528 138489 40 \-13.06 *F 27.00 Prom \+ 138746 138785 40 \-7.86 *F 27.01 Init \+ 138910 139511 602 0 2 64 78 896 0.980 80.96 *F 27.02 Intr \+ 139572 139803 232 0 1 61 127 130 0.967 11.98 *F 27.03 Term \+ 139860 139946 87 2 0 48 42 102 0.958 \-0.74 *F 27.04 PlyA \+ 139950 139955 6 \-9.23 *F 28.04 PlyA \- 139964 139959 6 \-3.44 *F 28.03 Term \- 140280 139991 290 1 2 20 32 378 0.944 20.94 *F 28.02 Intr \- 140515 140338 178 1 1 62 86 161 0.557 12.79 *F 28.01 Init \- 140605 140588 18 1 0 49 93 8 0.942 \-2.72 *F 28.00 Prom \- 140963 140924 40 \-6.96 *F 29.00 Prom \+ 141010 141049 40 \-9.46 *F 29.01 Init \+ 141294 141929 636 2 0 56 109 1138 0.999 108.04 *F 29.02 Intr \+ 141993 144206 2214 2 0 49 94 1995 0.999 183.54 *F 29.03 Term \+ 144404 144757 354 1 0 83 43 432 0.995 32.69 *F 29.04 PlyA \+ 144861 144866 6 \-1.95 *F 30.06 PlyA \- 144881 144876 6 \-4.73 *F 30.05 Term \- 145264 145058 207 1 0 115 49 436 0.999 39.74 *F 30.04 Intr \- 145923 145594 330 0 0 33 101 546 0.970 46.23 *F 30.03 Intr \- 146174 145977 198 2 0 35 58 244 0.943 15.65 *F 30.02 Intr \- 146366 146251 116 0 2 67 111 60 0.976 6.37 *F 30.01 Init \- 146573 146435 139 2 1 105 81 235 0.897 22.80 *F 30.00 Prom \- 146905 146866 40 \-5.36 *F 31.00 Prom \+ 149718 149757 40 \-4.66 *F 31.01 Init \+ 152206 152307 102 0 0 42 28 99 0.131 \-0.46 *F 31.02 Intr \+ 157413 157572 160 2 1 51 98 262 0.968 23.06 *F 31.03 Term \+ 157707 157837 131 1 2 93 47 190 0.898 13.74 *F 31.04 PlyA \+ 158517 158522 6 1.05 *F 32.00 Prom \+ 160340 160379 40 \-7.36 *F 32.01 Sngl \+ 160961 161119 159 1 0 40 52 338 0.958 17.91 *F 32.02 PlyA \+ 161244 161249 6 1.05 *F 33.00 Prom \+ 163071 163110 40 \-3.36 *F 33.01 Init \+ 163601 163661 61 1 1 67 103 22 0.971 3.01 *F 33.02 Intr \+ 163721 164271 551 0 2 79 105 803 0.999 73.69 *F 33.03 Intr \+ 164341 164535 195 0 0 \-12 83 333 0.986 22.41 *F 33.04 Term \+ 164594 164851 258 1 0 90 38 268 0.739 17.55 *F 33.05 PlyA \+ 164853 164858 6 \-7.21 *F 34.02 PlyA \- 164871 164866 6 \-1.95 *F 34.01 Sngl \- 165712 164924 789 1 0 67 43 1293 0.939 118.63 *F 34.00 Prom \- 166119 166080 40 \-10.84 *F 35.12 PlyA \- 166135 166130 6 1.05 *F 35.11 Term \- 166481 166338 144 2 0 63 32 110 0.955 0.81 *F 35.10 Intr \- 167436 166543 894 0 0 69 79 765 0.942 65.21 *F 35.09 Intr \- 167956 167494 463 0 1 6 23 451 0.964 23.56 *F 35.08 Intr \- 168426 168027 400 1 1 62 99 400 0.949 32.06 *F 35.07 Intr \- 168880 168483 398 0 2 54 75 379 0.997 27.42 *F 35.06 Intr \- 169108 168936 173 1 2 48 63 134 0.997 5.64 *F 35.05 Intr \- 169244 169167 78 2 0 72 97 101 0.997 9.15 *F 35.04 Intr \- 169438 169312 127 0 1 94 39 71 0.999 3.48 *F 35.03 Intr \- 169771 169498 274 2 1 19 52 273 0.967 13.40 *F 35.02 Intr \- 170045 169826 220 2 1 36 39 92 0.739 \-3.03 *F 35.01 Init \- 170354 170103 252 2 0 49 21 249 0.796 11.66 *F 35.00 Prom \- 170674 170635 40 \-9.26 *F 36.00 Prom \+ 170799 170838 40 \-8.06 *F 36.01 Init \+ 171022 171169 148 0 1 46 61 225 0.977 15.85 *F 36.02 Intr \+ 171232 172166 935 2 2 17 48 927 0.858 72.52 *F 36.03 Intr \+ 172227 172394 168 2 0 70 111 242 0.884 24.84 *F 36.04 Intr \+ 172451 173614 1164 1 0 19 80 1004 0.998 81.65 *F 36.05 Term \+ 173676 173972 297 2 0 89 38 257 0.999 16.07 *F 36.06 PlyA \+ 173985 173990 6 \-0.45 *F 37.09 PlyA \- 174027 174022 6 1.05 *F 37.08 Term \- 176334 175557 778 2 1 59 43 1301 0.998 115.54 *F 37.07 Intr \- 176708 176396 313 0 1 61 89 429 0.961 35.54 *F 37.06 Intr \- 177072 177004 69 1 0 80 95 32 0.802 2.25 *F 37.05 Intr \- 178106 177663 444 0 0 58 86 260 0.692 16.17 *F 37.04 Intr \- 195453 193760 1694 2 2 60 94 1414 0.235 126.52 *F 37.03 Intr \- 196795 196583 213 0 0 115 20 298 0.012 23.53 *F 37.02 Intr \- 197171 196985 187 0 1 72 101 263 0.978 24.75 *F 37.01 Init \- 197349 197235 115 0 1 27 68 193 0.974 11.57 *F 37.00 Prom \- 197861 197822 40 \-5.56 *F 38.00 Prom \+ 198781 198820 40 \-10.35 *F 38.01 Init \+ 199369 199494 126 0 0 44 107 5 0.343 \-1.84 *F 38.02 Term \+ 199556 200443 888 1 0 \-4 41 707 0.947 49.26 *F 38.03 PlyA \+ 200533 200538 6 1.05 *F 39.02 PlyA \- 200548 200543 6 \-1.75 *F 39.01 Sngl \- 203675 200769 2907 2 0 72 39 3147 0.992 298.73 *F 39.00 Prom \- 203750 203711 40 \-6.16 *F 40.00 Prom \+ 203776 203815 40 \-8.56 *F 40.01 Sngl \+ 204144 205238 1095 2 0 81 41 797 0.999 71.30 *F 40.02 PlyA \+ 205556 205561 6 1.05 *F 41.02 PlyA \- 206100 206095 6 1.05 *F 41.01 Sngl \- 206977 206438 540 1 0 46 43 884 0.999 75.79 *F 41.00 Prom \- 208451 208412 40 \-3.16 *F 42.00 Prom \+ 208544 208583 40 \-10.45 *F 42.01 Init \+ 208744 209994 1251 0 0 49 105 1328 0.839 123.13 *F 42.02 Intr \+ 212837 212975 139 1 1 112 97 243 0.997 27.64 *F 42.03 Intr \+ 213047 213290 244 0 1 67 81 510 0.999 44.76 *F 42.04 Intr \+ 213362 213533 172 2 1 35 91 334 0.610 28.35 *F 42.05 Intr \+ 213843 214142 300 2 0 47 115 594 0.515 54.93 *F 42.06 Intr \+ 214382 216368 1987 1 1 63 105 2327 0.951 219.23 *F 42.07 Intr \+ 216403 216500 98 2 2 62 115 28 0.823 2.63 *F 42.08 Intr \+ 216562 216777 216 0 0 58 92 176 0.811 13.70 *F 42.09 Intr \+ 216840 218064 1225 2 1 49 95 1704 0.601 154.50 *F 42.10 Intr \+ 218481 218596 116 1 2 20 92 122 0.965 5.97 *F 42.11 Intr \+ 218663 219238 576 1 0 64 91 500 0.974 40.82 *F 42.12 Intr \+ 219375 219564 190 2 1 54 98 293 0.625 26.06 *F 42.13 Intr \+ 219675 224662 4988 1 2 95 75 5546 0.997 541.45 *F 42.14 Intr \+ 224733 224909 177 2 0 49 113 279 0.977 26.62 *F 42.15 Term \+ 224996 225034 39 1 0 77 41 60 0.885 \-2.61 *F 42.16 PlyA \+ 225843 225848 6 1.05 *F Predicted peptide sequence(s): *F Predicted coding sequence(s): *F >AE003746|GENSCAN_predicted_peptide_1|351_aa *F MKMVDKSSKLVDKFAVISGTGGSGPGGGPLNLQSGGGGGGSGGGGGVSGSGGISGTAGLS *F AGAGMTTGQKPVPMKLFAAWEVDRTPPNCIPSAAGAAAAADAFPLSQRHLCIIEITGDES *F AAVLLAMTDDSGLRVWVTRVFVELANLLVIRVDGKVQQESQLNSMYASINQQPSHENVFA *F TVDSSSVLVELLLPALDFPFSISPSVFVCTRVQGEGEGAGVEAWKLEVPRHCPGMSTSSL *F LTRPATCAQVEFCSHTNNDRITANSGNTNGCSNSYTNNSHYNNAKDGSNCNNSRKQRASW *F RKLPLPQVPSSQFHVPGSMLAKSRQAKRATDVSCINRSPPDPQLYLHPRLR *F >AE003746|GENSCAN_predicted_CDS_1|1056_bp *F atgaagatggtcgataagtcgtccaagttggtggataaatttgccgtaatcagcggcacc *F ggcggcagcggccctggaggcggtccgctcaacctgcaaagtggcggaggaggcggcggt *F tccggcggcggaggaggagtctccggatctggtggcatatccggaacggctggcttgtcc *F gccggagcaggaatgaccactggccagaaaccggtgcccatgaagctattcgccgcctgg *F gaagtggaccgaacgccgcccaattgcatcccaagtgctgctggtgctgctgctgctgct *F gatgcttttccgctgtcccaacgccatctgtgcatcatcgagattaccggcgacgagtca *F gcagctgtgcttttggccatgaccgatgattcaggtcttcgagtgtgggtcacgcgggtg *F tttgtggagctggcaaatctcctggtcattagggtcgatggaaaagtgcagcaggaaagt *F caattaaattcgatgtatgccagcatcaaccagcagccgagccatgagaatgtcttcgcg *F accgttgactcatccagtgtgttggtcgaactgcttctgcctgcactggattttccattt *F tccatttccccctcggtttttgtttgcactcgcgtccaaggcgaaggcgaaggtgctggc *F gtggaagcgtggaaacttgaagtaccacgacattgtccaggcatgtcaacgtcaagtttg *F ttgactcgcccggcaacctgcgcccaagtggagttttgcagccacaccaacaacgaccgt *F atcaccgcaaacagcggcaacacgaacgggtgcagcaacagctacaccaacaacagtcac *F tacaacaacgccaaagacggcagcaactgcaataacagccgcaaacaacgagcaagttgg *F cgaaaacttccgctcccccaagttcccagttcccagttccatgttccaggttccatgctg *F gccaaaagcagacaggccaaacgagctacggatgtcagctgcataaatcgttcgcctcca *F gacccccagttgtaccttcacccccgccttcgatga *F >AE003746|GENSCAN_predicted_peptide_2|608_aa *F MADAWDIKSLKTKRNTLREKLEKRKKERIEILSDIQEDLTNPKKELVEADLEVQKEVLQA *F LSSCSLALPIVSTQVVEKIAGSSLEMVNFILGKLANQGAIVIRNVTIGTEAGCEIISVQP *F KELKEILEDTNDTCQQKEEEAKRKLEVDDVDQPQEKTIKLESTVARKESTSLDAPDDIMM *F LLSMPSTREKQSKQVGEEILELLTKPTAKERSVAEKFKSHGGAQVMEFCSHGTKVECLKA *F QQATAEMAAKKKQERRDEKELRPDVDAGENVTGKVPKTESAAEDGEIIAEVINNCEAESQ *F ESTDGSDTCSSETTDKCTKLHFKKIIQAHTDESLGDCSFLNTCFHMATCKYVHYEVDTLP *F HINTNKPTDVKTKLSLKRSVDSSCTLYPPQWIQCDLRFLDMTVLGKFAVVMADPPWDIHM *F ELPYGTMSDDEMRALGVPALQDDGLIFLWVTGRAMELGRDCLKLWGYERVDELIWVKTNQ *F LQRIIRTGRTGHWLNHGKEHCLVGMKGNPTNLNRGLDCDVIVAEVRATSHKPDEIYGIIE *F RLSPGTRKIELFGRPHNIQPNWITLGNQLDGIRLVDPELITQFQKRYPDGNCMSPASANA *F ASINGIQK *F >AE003746|GENSCAN_predicted_CDS_2|1827_bp *F atggcagatgcgtgggacataaaatcactcaagacaaagcggaacacgctccgcgagaag *F ctggaaaagcgcaagaaggagcgcattgagattctctcggacattcaggaggatctgacg *F aatcccaaaaaggaactcgttgaggctgacttggaagtacagaaggaggtgctgcaggcc *F ctcagttcctgctccctggccctgcccattgtctccacacaggtggtggaaaagattgcc *F ggcagcagcctggagatggtgaactttattctaggcaaactggccaaccagggtgcaatt *F gtgatccggaacgtgaccatcggcaccgaggccggctgcgaaatcatctctgtgcagccc *F aaggagctgaaggagatcctggaagacaccaacgatacgtgtcagcaaaaggaggaggag *F gccaagagaaagttggaagtcgacgatgttgatcagccacaggagaagacaataaaactg *F gagtccactgtagcgcggaaagagtccacaagcctggatgctccggacgatattatgatg *F ctgctatccatgccttccacccgcgaaaaacagagtaagcaggtgggcgaggagattctt *F gagctgcttactaagcctacggccaaagagcgatccgtggctgagaagtttaagtcccac *F ggcggagcgcaggtcatggagttttgctcccacggcacaaaagtcgagtgcctgaaggct *F caacaagccaccgccgagatggcggccaaaaagaaacaagaaagaagagacgaaaaggag *F cttcgcccggatgttgatgcgggcgaaaacgtcaccggtaaggtacccaagacagaatcg *F gcagccgaggatggagaaatcattgcggaggttataaacaattgcgaagccgagtcgcag *F gaatccaccgatggcagcgatacctgcagcagtgagacaacagacaagtgcaccaaactg *F catttcaaaaagatcatccaggctcacacggacgaatctctgggggactgcagtttccta *F aacacctgcttccacatggccacctgcaagtacgtgcactatgaggtggacaccctgccg *F catataaacacaaacaagcccacggatgtgaaaaccaaattgagcctcaagcgcagcgta *F gactccagttgcacgctctatccgccgcaatggattcaatgcgacctacgcttcttggac *F atgacggtcctgggaaagttcgctgtggtgatggccgatcctccctgggatatccacatg *F gaattgccctacggcacaatgtcggacgatgaaatgagagcactgggcgtgccggcactt *F caggatgacggcttgatctttctgtgggtcactggacgagccatggaacttggccgcgac *F tgcctcaagttgtggggctatgagcgcgtggacgaactcatctgggtaaagaccaaccaa *F ctgcagcgaattattcgcactgggcgcactggtcattggctaaaccacggcaaggagcac *F tgtttggtgggcatgaaaggcaatcccacaaacctgaaccgcggactcgattgcgatgtg *F atcgttgcggaggtgcgggccacctctcacaagccggatgagatttatggtattatcgag *F cgtctaagcccgggtactcgcaagatcgagctctttgggcgtccgcacaacatccaaccg *F aactggataaccctgggaaaccaactggatggcattcgactggtggatccggagctaatt *F acgcagttccagaagcgttatccagatggtaactgcatgtcgccagcttctgccaatgcg *F gcgtcgatcaatggaatacaaaagtag *F >AE003746|GENSCAN_predicted_peptide_3|608_aa *F MGQYTASQRKNVRILLVGDAGVGKTSLILSLVSEEYPEEVPPRAEEITIPANVTPEQVPT *F SIVDFSAVEQSEDALAAEINKAHVVCIVYAVDDDDTLDRITSHWLPLVRAKCNPSLDGEG *F DAEAEAEGDTQREPIRKPIVLVGNKIDLIEYSTMDSVLAIMEDYPEIESCVECSAKSLHN *F ISEMFYYAQKAVLHPTSPLYMMEEQELTSACKKSLVRIFKICDIDGDNLLNDYELNLFQR *F RCFNTPLQPQILDEVKAVIQKNVPDGIYNDAVTLKGFLFLHCLFIQRGRNETTWAVLRRF *F GYNDQLEMCQEYLRPPLKIPPGSSTELSHRGQQFLIAVFERYDRDGDGALSPEEHKMLFS *F TCPAAPWSYSTDIRKSCPINETTGWVTLHGWLCRWTLMTLIDVVKTMEYLAYLGFNVHEN *F DSQLAAIHVTRERRIDLAKRQSSRSVYKCHVIGPKGSGKTGMCRGFLVEDMHKLIGKEFK *F TNVVNCINSVQVYGQEKHLILRDIDVRHALDPLQPQEVNCDVACLVYDSSNPRSFEYVAR *F IYIKYYAESKIPVMIVGTKCDMDERRQDYLMQPSEFCDKYKLLPPHLFSLKTNKKELYTK *F LATMAAFP *F >AE003746|GENSCAN_predicted_CDS_3|1827_bp *F atgggacagtacacggcgtcgcagcgcaagaatgttaggatcctgctcgtcggcgacgcc *F ggggtgggtaagacgtcgttgattctgtctctggtcagtgaggagtacccggaggaggtt *F ccgcctagggctgaggagattaccattccggcgaacgtgacgcccgagcaggtgcccacc *F agcatagttgacttctcggccgtggagcagtcggaggatgccctggccgccgaaattaac *F aaggcgcacgtggtgtgcatagtgtacgccgtggacgatgacgacactttggatcggatc *F acatcccattggctgccgctcgtccgggccaagtgcaatccctccctggacggcgagggt *F gatgccgaagcggaggcggaaggagacacacaacgagagcccattcggaagccaatcgtt *F ctggtgggtaacaaaatagacctcatcgagtattccaccatggacagcgtgctggccatc *F atggaagactaccccgagattgagagttgcgtggagtgctctgccaagtcgctgcacaac *F atctccgagatgttttactacgcccagaaggctgtgctgcacccgacttctcccctgtat *F atgatggaagaacaggagctcacatccgcctgcaagaagtcgctggtgcgcattttcaag *F atctgcgacattgacggggacaatctgttgaacgattacgagctgaatttattccagcga *F cgctgtttcaacacaccactccagccgcaaatccttgatgaggtgaaggccgtgatacag *F aaaaatgtgcccgatggcatatacaacgatgcggtcaccctgaagggcttcctcttctta *F cactgccttttcattcagcggggaaggaacgagacaacctgggcggtgttacggcgcttt *F ggctataacgaccagttggagatgtgccaggagtatcttaggccaccgctgaaaataccg *F cctggcagcagcacagaactctcgcaccgcggacagcagtttctgattgccgtgtttgag *F cgctatgatcgcgatggcgacggagccttatcacctgaggagcacaagatgctcttcagc *F acatgcccggctgctccgtggtcctactccaccgacatacgcaagtcctgcccgatcaac *F gagacaactggatgggtgacactgcacgggtggctatgtcggtggacactgatgacgctg *F attgatgtggtcaaaacaatggagtatttggcctatttgggcttcaatgttcatgaaaac *F gacagccagttggcggcaattcacgtaactcgagagcgccgcatcgatttggccaagcgc *F caaagcagtcggtccgtttacaagtgtcatgtgattggaccaaagggatcaggaaagact *F ggaatgtgcaggggattcctggtggaggatatgcacaaactaatcggaaaagagtttaaa *F acgaatgtggttaattgcatcaactctgtgcaggtgtatggccaggaaaagcacctcatt *F ctgcgtgacatagacgtcaggcatgccctcgatcccctccagccacaagaagtcaattgc *F gatgttgcctgcctagtctacgactcatctaatccccgttcctttgagtacgtggcccgc *F atctacatcaagtattatgcggagagcaagattccagtgatgatagtcggcaccaagtgc *F gacatggacgaacgccggcaggactatctaatgcagccctctgaattctgtgacaagtac *F aagttgctgcctccgcatctcttcagcctgaaaaccaacaaaaaagaactgtataccaag *F ctggccacaatggcagcgtttccgtga *F >AE003746|GENSCAN_predicted_peptide_4|758_aa *F MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSA *F AGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSF *F PIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPS *F ELNREGDGQEQQLSNQPQRFRPIQPLEMAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYI *F SKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSM *F ARDRLHFLALREQDLQMQRLSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYG *F PKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPP *F AVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKV *F EWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATEC *F SATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASR *F RLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLL *F NRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMR *F AITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI *F >AE003746|GENSCAN_predicted_CDS_4|2277_bp *F atggtacgcactaaaaaccagtcgtcctcctccagcgccagcagcagcagcaccaagtca *F ccaataaagtccagcagcggggcaggatcatccggcggaggactcggtggtcgccagtcc *F actcaccgctcgtcgagcgcctcaaacgttgctgccgttgttgccggcggttcatctgcc *F gccggtggaggctcctcatcgaatcgccgtagtccgggcagctcacccgacggcgacgac *F gacaccaccaccacagatgacctgacccccacaacgtgctccccacgcagcggccaccac *F cattcgtacggcggctactcgtcctctgtgcacaaacagaacctctacgtcgtctcgttc *F cccataatctttcttttcaacgttctgcgctcgctgatttatcagctgttttgtattttt *F cgctacctatacggtgcgagcacaaaggtgatataccgcccgcaccgacgcgactgtaat *F attgaaatcgtggtgcagaattcctccaaggagcaacagcagtcgttgaaccatccttcg *F gagctgaaccgtgaaggtgacggacaggaacagcagttatccaatcagccacagcgtttc *F aggcccattcaaccgttggagatggccgccaatcgtccgggaggagggtattcacctggt *F cccggcgatccattgctggccaagcagaagcatcaccatcgacgcgccttcgagtacatc *F tccaaggcgctcaaaattgatgaggagaacgaaggtcacaaagagctggcaattgagctc *F taccgaaaaggtatcaaggagctagaggatggcatagctgtcgattgctggagtggacgt *F ggtgatgtgtgggatcgggctcagcgcctgcacgacaagatgcagacaaacctttcgatg *F gcgcgggatcgtctccattttctagctctgcgtgagcaggatttgcaaatgcagcgcctc *F tccttaaaggagaagcagaaagaagaggctcaaagcaagccgcagaagaccagggagccc *F atgctggcaggaatgaccaacgaaccaatgaaactaagggtgcgcagcagtggctatggg *F cccaaggccaccaccagtgcccaacccactgcatctggccgcaaactgacaattggatcc *F aaacgacccgtcaacttggccgtggccaacaaatcacaaacgctgccccgcaaccttggc *F tccaaaacatctgtaggcgcagtccaaaggcaaccagccaagacagctgcaacgcctccc *F gccgttcggcgacaattttcttcggggcgtaatacgccgccccagcgttcgcggactccc *F ataaacaacaacggacccagtggcagtggcgccagcactccggtggttagtgtgaaggga *F gtggaacagaagctggtgcaacttatactcgacgagatcgtcgagggtggggccaaagtg *F gagtggactgacattgccggtcaggatgtggccaagcaggctctacaggaaatggtcatt *F ctaccctccgtccgaccggaactctttacggggcttcgtgcaccggctaagggtttattg *F ctgtttggtcctcccggaaatggcaagacactgctggcccgcgccgtggctactgagtgt *F agtgccaccttcctgaacatttcggctgcctcgctgaccagcaagtacgtgggggatggc *F gagaaactggtgagggctctcttcgccgtggcccgtcacatgcagccctccattattttc *F atcgacgaggtggactcgttgctttcagagcgaagcagcagcgagcatgaagcatcgcgt *F cgcctaaagaccgagtttctagtggagttcgatgggctgcctggaaatccagacggagac *F aggatcgtggtgctggccgccaccaatcgaccgcaggaattggacgaggcagccctgcgt *F cggttcacaaagcgcgtttacgtctcactgcccgacgagcaaacccgcgagctgctcctc *F aatcgactgctccagaagcaaggcagtccgttggataccgaggcgttgcgtcgccttgca *F aagataacagacggatactcgggctccgacttaacggcactggccaaggacgccgcattg *F gagcccattcgagagttgaatgtggagcaagtgaagtgtctggacatcagtgcaatgcgt *F gcaatcacagagcaggactttcacagttcgctcaagcgcatcaggcgctcggtggcgccg *F caaagcctcaactcatatgagaagtggtcgcaagattatggcgacatcaccatctag *F >AE003746|GENSCAN_predicted_peptide_5|490_aa *F MDESQPKTLYVGNLDSSVSEDLLIALFSTMGPVKSCKIIREPGNDPYAFIEYSNYQAATT *F ALTAMNKRLFLEKEIKVNWATSPGNQPKTDISSHHHIFVGDLSPEIETETLREAFAPFGE *F ISNCRIVRDPHTMKSKGYAFVSFVKKAEAENAIQAMNGQWIGSRSIRTNWSTRKLPPPRE *F PSKGGGQGGGMGGGPGNGSGVKGSQRHTFEEVYNQSSPTNTTVYCGGFPPNVISDDLMHK *F HFVQFGPIQDVRVFKDKGFSFIKFVTKEAAAHAIEHTHNSEVHGNLVKCFWGKENGGDNS *F ANNLNAAAAAAAASANVAAVAAANAAVAAGAGMPGQMMTQQQIAAATGAAIPGQMMTPQQ *F IAAQYPYAYQQMGYWYPPATYPTTQMQTQYMQQGYYPYAYPTSAQQAGGVPCIQFSAAGY *F RMVPPNVAWGVPGTVVPVLRCHNTRPNELRCDASFDAVCSIREVAPSRSHNHLCCISFRT *F CSSCKRITHV *F >AE003746|GENSCAN_predicted_CDS_5|1473_bp *F atggacgagtcgcaaccgaagaccctatacgtgggcaacctggatagctcagtgtccgag *F gacctgctaattgccctcttcagcaccatggggcccgtcaaaagctgcaaaatcattcgg *F gaaccgggcaacgatccatatgccttcatcgaatattccaactaccaggcagccacaaca *F gctctgaccgccatgaataaacgcctattcctcgaaaaggaaatcaaggtcaactgggcc *F accagtcccggcaatcagccgaagacagacatcagttcgcaccaccacatattcgtgggc *F gacctcagtcccgagattgagacagaaacactgcgcgaggctttcgccccattcggagag *F atctccaactgtcgcattgtgcgcgaccctcacaccatgaagtcaaagggttacgccttc *F gtgtcgtttgtgaaaaaggcggaggcagagaacgccatccaggcgatgaacggccagtgg *F attggctcgcgctcgatacgcaccaactggtccacgcgcaagctgccaccaccacgcgag *F ccttccaagggcggaggccagggaggcggaatgggtggcggaccgggcaatgggtccggt *F gtaaagggaagtcaacgccacaccttcgaggaagtgtataaccagtcgagccccaccaac *F accaccgtatactgtggcggattcccgccgaatgtcatcagtgacgacctgatgcacaag *F cacttcgtccagtttggtcccatccaggacgtgcgggtcttcaaggacaagggcttctcg *F ttcatcaagtttgttaccaaggaggcagccgcccacgccatcgagcacacgcacaacagc *F gaggtacatggaaacctggtaaagtgcttctggggcaaagagaacggaggcgataactcg *F gccaataacctcaatgccgccgctgccgcggcagcagcctctgccaatgttgccgccgtt *F gcggcagccaatgctgcggttgccgctggagcgggtatgcccggtcagatgatgacgcag *F caacagatcgccgccgcaacaggagcggcgatacccggccaaatgatgacaccccagcag *F attgcggcgcagtatccatacgcgtaccagcagatgggctactggtatccacctgcgact *F tatcccacaacccagatgcagacgcaatacatgcagcagggctactatccctacgcctac *F cctaccagtgctcagcaagcgggaggagtcccttgcatacaattttcagcggctggatac *F cgcatggtgccgccgaatgtagcatggggcgtgcccggaactgtggtgcccgtgctgcga *F tgccacaataccagacccaatgagctgagatgtgacgccagcttcgacgccgtttgcagc *F atccgggaagtcgcccccagccgcagccacaaccacctctgctgcatcagcttccgcacc *F tgcagcagttgcaagcgcattacgcacgtttag *F >AE003746|GENSCAN_predicted_peptide_6|232_aa *F MGINIFINSKYIISCGDHVKCDELYSRIAEKTNLQPGEYYLVSNGKRLEGEISSGDVHCV *F LRQLGGKGGFGSMLRAIGAQIEKTTNREACRDLSGRRLRDINEEKRVRAWLEKQGERERE *F AEERKKRKIEKLLAVPKHDFKDDKYEEARANLTEKVNDAFEEGLKQAEENKEKGVEEATS *F SGTKRKSPAVDKTKAKKKKKGTLWIDDDISGSDSDSDDSEEEPKTQKKAIQN *F >AE003746|GENSCAN_predicted_CDS_6|699_bp *F atgggcataaatatttttataaacagtaaatatataatttcttgtggagatcacgttaaa *F tgtgatgagctttacagtcggattgcggagaaaacaaacctgcaaccaggggaatactac *F ttagtcagcaatggcaagcgcttggagggagaaatttcatctggagatgttcactgcgtt *F ctccgccagctcggtggcaaaggaggatttggttccatgcttcgagccattggtgcgcaa *F attgaaaagacaaccaatcgcgaggcttgccgcgatctaagtggtcgccgtttgcgggat *F atcaacgaggagaagcgggtgcgcgcctggctggagaagcagggcgaacgggaacgggag *F gccgaggagcgtaaaaagcgaaaaatcgagaaactgctggccgtgcccaagcacgacttt *F aaggacgataagtacgaggaagccagggctaatctgaccgagaaggtaaacgacgccttc *F gaggagggactcaagcaggccgaggagaacaaggagaaaggcgtcgaggaagcaacttcc *F agtggcacaaagaggaaatcccccgccgtagacaagaccaaggcaaaaaagaagaaaaag *F ggcacactctggatagacgatgacatctctggatccgactctgactctgacgacagcgag *F gaggagccaaaaacacagaaaaaagctatacaaaactag *F >AE003746|GENSCAN_predicted_peptide_7|388_aa *F MSEAEKQAVSFACQRCLQPIVLDEQLEKISVHAMAELSLPIYGDNGNTLDPQDASSFDHF *F VPPYRLTDSINGTGFMLVSDGRDNKKMSAAFKLKAELFDCLSSNSEIDHPLCEECADSML *F EIMDRELRIAEDEWDVYKAYLDELEQQRVAPNVEALDKELDELKRSEQQLLSELKELKKE *F EQSLNDAIAEEEQEREELHEQEESYWREYTKHRRELMLTEDDKRSLECQIAYSKQQLDKL *F RDTNIFNITFHIWHAGHFGTINNFRLGRLPSVSVDWSEINAAWGQTVLLLSALARKIGLT *F FERYRVVPFGNHSYVEVLGENRELPLYGSGGFKFFWDTKFDAAMVAFLDCLTQFQKEVEK *F RDTEFLLPYKMEKGKIIDPSTGNSYSIK *F >AE003746|GENSCAN_predicted_CDS_7|1167_bp *F atgagtgaggcggaaaagcaggcggtgtccttcgcctgtcagcgctgcctgcagcccatc *F gtcctggacgagcagctggagaagattagcgtgcacgcaatggcggagttatctttgccc *F atctacggagacaatggcaatacattggacccgcaggacgccagcagcttcgaccacttt *F gtgccgccctacaggcttacggactctataaatggcactggttttatgctggtttccgat *F ggcagggacaacaagaaaatgagtgctgcttttaagctgaaagcggagctgtttgactgc *F ctctcctccaactctgagattgaccatccgctgtgcgaagagtgtgccgactccatgctg *F gaaatcatggacagggaactgcgcatcgctgaggacgagtgggatgtgtacaaggcttat *F ttggatgaactagagcaacagcgtgtagcacccaacgttgaggccctggacaaggagctc *F gacgaactaaagcgcagcgagcaacagcttctgtcggagctaaaagagctcaaaaaggag *F gaacaatcgctaaatgatgccattgccgaggaggaacaggagcgagaggagctgcacgag *F caggaggaaagctactggcgcgagtataccaagcacaggcgtgagctaatgctcaccgag *F gatgacaaacgaagtctggagtgtcagatcgcctactcgaaacagcagctggacaaactg *F cgcgacaccaacatattcaacatcacctttcacatctggcatgccgggcatttcggtacc *F attaataactttaggctgggtcgattgccctctgtatccgtggactggtcagagatcaac *F gccgcttggggccagacggtgcttctgctctctgcgcttgctcgtaagatagggctcacc *F ttcgagcggtatcgtgtagtaccctttggaaatcattcgtatgttgaggtgctcggcgag *F aatcgagagctcccgctttatggcagcggcggatttaagtttttctgggacaccaagttt *F gatgctgccatggtagcttttctcgactgtcttacccagttccagaaggaggtcgagaag *F cgcgacaccgagttcctgctgccctacaagatggagaagggcaagatcatcgatccctcc *F acgggaaattcctattctattaagtag *F >AE003746|GENSCAN_predicted_peptide_8|447_aa *F MVSYFVPRGRFLLKAGNLRQVVQQQHQPAQLQLQPIKGPQPQAQNASLPVARHLRQFSSN *F PASKEAPLHHRRPQHKQQPNPSQELAQIRRNILSRWTGFLLRWAPMGICVFGAIEWQLQK *F NRCEKEGKPRTASELQSRIYCSLPLRIISRCWGWLAACYLPPSLRPYVYGWYSNTFDVNL *F SEAMYPEYEHYNSLAEFFTRPLKEGVRVIDQQAPLVSPADGKVLHFGSASDSLIEQVKGV *F SYSIEDFLGPLETVEQANSGASYAQALKKKSDGSTELYQCVIYLAPGDYHRFHSPTAWKP *F TIRRHFSGELLSVSPKVAGWLPGLFCLNERVLYMGQWKHGFFSYTAVGATNVGSVEIYMD *F ADLKTNRWTGFNVGKHPPSTYEYDELVLNKELTEAPKEFGKGDLVGQFNMGSTIVLLFEA *F PKNFKFDIIAGQKIRVGESLGHIVGSK *F >AE003746|GENSCAN_predicted_CDS_8|1344_bp *F atggtttcctacttcgtacctcgtggccgcttcctgctgaaggccggcaatctcagacag *F gtggtgcagcagcagcatcagccggcccagctacagctgcagcccattaaaggaccacag *F ccgcaggctcagaatgccagtctgccggtggcccgtcacttgcgccaattctcctcgaat *F ccagcctccaaggaggcaccattacaccaccggcgcccgcaacataaacaacaaccgaat *F cctagtcaggagttagctcagattcggcgcaatatactctcacggtggacgggcttcctg *F ttgcgttgggctcccatgggcatctgtgtgtttggcgccatcgagtggcagttgcagaag *F aaccgctgcgaaaaggaaggaaaacctcggacagcgtccgagctccagtcgcgcatttac *F tgctccctgccactgcgcataatcagccgttgctggggctggctggccgcttgctacttg *F cctcccagtctgcgtccctacgtctacggatggtattccaacacgtttgatgttaatttg *F agcgaggccatgtatccggagtatgagcactacaatagtctggctgagttctttaccagg *F ccacttaaggagggcgttcgtgtcatcgatcagcaggctccactggtctcccccgccgac *F ggtaaggttctacattttggcagcgcctcggactcgctaatagagcaggtcaagggtgtt *F agctacagtatcgaggacttccttggcccgctggagactgtggagcaggcaaattccggt *F gcctcctatgcccaggccctcaaaaagaagagcgatggttctacggagctgtaccagtgc *F gtgatatatctggctcccggagattaccatcgattccactctcctaccgcttggaagccc *F accattcgtcgtcacttctccggcgaactgctgtccgtgagccccaaagttgccggctgg *F ctgcctggtctgttttgcctcaacgaacgtgtgctgtacatgggccagtggaagcacgga *F ttcttctcctacaccgccgtgggtgccacaaacgtgggatccgtcgaaatctacatggat *F gccgatctgaagacgaaccgttggactggattcaatgtcggcaagcatccgcccagcacc *F tacgagtacgatgaactggtcttgaacaaagaactaacagaagcgcccaaggaattcggc *F aagggggatctggtgggccagttcaatatgggcagcaccatagtcctgctcttcgaggcg *F ccaaagaatttcaaatttgatattatcgccggtcagaagatccgcgttggcgagtccctc *F ggccacatcgtcggctccaaatga *F >AE003746|GENSCAN_predicted_peptide_9|147_aa *F MALRVARSQIPFSTARNTQSNLLQRFYSQAPQIGIVDYDVVKKLPSEPQKLLIDVREPEE *F LKETGQIPASINIPLGVVSQELAASEQLFKSKYGREKPKPETEIIFHCKIGKRSLKAAEA *F AAALGFKNVKNYQGSWLDWAEREGLPK *F >AE003746|GENSCAN_predicted_CDS_9|444_bp *F atggccttgagggtcgctagatcccagatcccgttcagcactgccaggaatactcagagc *F aatctcctccaacgtttttacagccaggctcctcaaattggaatcgtggactacgacgtg *F gtcaagaaactacccagcgaaccacagaaactgctcatcgatgtccgggagccggaagag *F ctgaaggaaaccggacaaatccccgccagcatcaacatccctctgggcgtggttagtcag *F gaattggcggccagtgagcagctttttaaatccaaatatggccgggaaaaaccgaagcca *F gaaacggagattatattccactgcaagattggcaaaagaagccttaaggctgcagaagct *F gccgccgcattgggattcaagaatgtaaagaactaccagggatcttggctggattgggcc *F gaacgagagggcctgcccaagtaa *F >AE003746|GENSCAN_predicted_peptide_10|635_aa *F MPAIGIDLGTTYSCVGVFQYGKVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNP *F KNSVFDAKRLIGRRFDDSKIQEDIKHWPFKVINDNGKPKISVEFKGANKCFSPEEISSMV *F LTKMKETAEAYLGTTVKDAVITVPAYFNDSQRQATKDAGAIAGINVLRIINEPTAAALAY *F GLDKNLKGERNVLIFDLGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVNHFA *F EEFKRKYKKDLRSNPRALRRLRTAAERAKRTLSSSTEASLEIDALYEGHDFYSKVSRARF *F EELCGDLFRNTLEPVEKALKDAKMDKSQIHDIVLVGGSTRIPKVQNLLQNFFGGKTLNLS *F INPDEAVAYGAAIQAAILSGDKSSEIKDVLLVDVAPLSLGIETAGGVMTKLIERNSRIPC *F KQSKTFTTYADNQPAVTIQVFEGERALTKDNNVLGTFDLTGVPPAPRGVPKIDVTFDLDA *F NGILNVTAKEQGTGNAKNITIKNDKGRLSQADIDRMLSEAEKYAEEDERHRQRIAARNQL *F ETYLFGVKEAAENGGDRISAADKSSIVERCSEAMKWLDSNTTAEKEEYEYKLKELEQFCS *F PIMTKMHKGGGDGQQAPNFGQQAGGYKGPTVEEVD *F >AE003746|GENSCAN_predicted_CDS_10|1908_bp *F atgccagccattggaatcgatttgggcaccacatactcctgcgtgggagtcttccagtac *F ggaaaagtggagatcattgccaacgaccagggtaaccgtaccacaccatcgtacgtggcc *F ttcaccgactcggaacgccttattggagatgccgccaagaaccaggtggccatgaacccc *F aagaactctgtgttcgatgccaaacgcctgattgggcgtcgtttcgacgattccaagatc *F caggaggacattaagcactggccgttcaaagtgatcaacgacaacggcaaaccaaagata *F agcgtggagttcaagggcgcgaataagtgcttctctcccgaggagattagctcgatggta *F ctcaccaaaatgaaggagactgcagaagcctacctgggcactacagtgaaggacgctgtc *F atcacagtgccggcctacttcaacgactcccagcgccaggcaacaaaggatgctggtgcc *F atcgctggcatcaatgtgctccgcatcatcaacgagccaacagcggcggctctggcctac *F ggcctggacaagaatctgaagggagagcgcaatgtgctgattttcgatttgggcggtggt *F acttttgacgtctccatcttgaccattgacgagggatccctgtttgaggtgcgctcgact *F gcgggagacacacatctgggtggcgaggacttcgacaaccgattggttaaccactttgcc *F gaggagtttaagcgcaagtacaaaaaggatctgcgctccaacccacgcgcactgcgtcgt *F cttcgcacggcagccgagcgcgcaaagcgcaccctttcctccagcacggaggcttctttg *F gagattgacgccctgtacgagggacacgacttctactcgaaggtgagccgcgccagattc *F gaggaactttgcggtgacctcttccgcaacacactggagccagttgagaaggcactcaag *F gacgctaaaatggacaagagccagatccatgacatagtcctggttggaggctccactcgt *F attcccaaggttcagaacctgctgcagaacttcttcggcggaaagaccctgaacttgtca *F atcaatccggacgaggcggtggcctacggagcagccatccaggcagccattctgtcgggc *F gacaagagcagcgagatcaaggatgtcctactggtcgatgttgccccactctcgttgggc *F atagaaaccgccggcggggtgatgaccaagctgattgagcgcaacagccgcattccatgc *F aagcagtccaagaccttcaccacctatgccgacaaccagccggcggtgaccattcaagtg *F tttgagggcgagagggctctgaccaaggacaacaatgtattgggcacattcgatctcact *F ggcgttccacccgcaccccgtggagtgcccaagatcgacgttactttcgatttggacgca *F aacggtatcctgaatgtgaccgccaaggagcagggcactggcaacgccaagaacattacc *F atcaagaacgacaagggtcgtctgtcgcaggcggacatcgaccgcatgctcagtgaggcg *F gagaagtacgccgaggaggacgagcgccatcgccagcggatcgccgcccgcaatcaactg *F gagacctatttgtttggtgtaaaggaggcggccgagaatggtggcgatcgcatcagtgca *F gccgacaagagcagtattgtggagcgttgcagcgaggcgatgaagtggttggacagcaac *F accaccgccgagaaggaggagtacgagtacaaactgaaggaactggaacagttctgcagc *F cccatcatgaccaagatgcacaagggaggtggagatggccagcaggctccaaactttgga *F cagcaagctggcggttataagggtcccaccgtcgaggaggtggactaa *F >AE003746|GENSCAN_predicted_peptide_11|1244_aa *F MTEEDDDVAQRVATAPVRKPDDETAFLEYIEMENFKSYRGHIVVGPLKQFNAVIGPNGSG *F KSNFMDAISFVMGEKTSSLRVKRLNDLIHGSSIGKPVSRSCYVTAKFVLNEERHMDFQRA *F VIGGSSEYRINGESVSSSTYLNKLEKIGINVKAKNFLVFQGAVENIAMKTPKERTALFEE *F ISGSGLLKDDYNRLKQEMIVAEEETQFTYQKKKGIAAERKEAKHEKMEADRYTRLQNEYN *F EKQVEYQLFRLFHVERDIRKFTSDLEVRQQEVKAVEQRKEAADEILREKKKDAGKITRDL *F AKIDQEIREFETQMNKRRPLYIKAKEKVTHCKKKLISLQKTLETAREADNAHQSDIRKLE *F KQLADVEALKKRFEDEIENESQRRGKSVNMEEGLVQEYDRLKQEAEATATQYRSELDSVN *F REQKSEQDTLDGETNRRASVEESFKKLTLQREEAVKRRDKLMDHIKSSQAALEEQNRIKD *F ELRRDVGTSKEKIAEKQRELEDVRDQLGDAKSDKHEDARRKKKQEVVELFKKQVPGVYDR *F MINMCQPTHKRYNVAVTKVLGKFMEAIIVDTEKTARHCIQILKEQMLEVETFLPLDYLQV *F KPLKERLRNISDPRNVRLVFDVLKFEPQEIERAVLFATGNALVCETPEDAMKVAYEIDRS *F RFDALALDGTFYQKSGLISGGSHDLARKAKRWDEKHMAQLKMQKERLQEELKELVKKSRK *F QSELATVESQIKGLENRLKYSMVDLESSKKSISQYDNQLQQVQSQLDEFGPKILEIERRM *F QNREEHIQEIKENMNNVEDKVYASFCRRLGVKNIRQYEERELVMQQERARKRAEFEQQID *F SINSQLDFEKQKDTKKNVERWERSVQDEEDALEGLKLAEARYLKEIDEDKEKMEKFKQDK *F QAKKQAVDDMEEDISKARKDVANLAKEIHNVGSHLSAVESKIEAKKNERQNILLQAKTDC *F IVVPLLRGSLDDAVRQSDPDVPSTSAAMENIIEVDYSSLPREYTKLKDDSAFKKTHEMLQ *F KDLQSKLDVLERIQTPNMKALQKLDAVTEKVQSTNEEFENARKKAKRAKAAFERVKNERS *F SRFVACCQHISDAIDGIYKKLARNEAAQAYIGPDNPEEPYLDGINYNCVAPGKRFQPMNN *F LSGGEKTIAALALLFSTHRYADIIFFHILRVNINIFLCSFHPAPFFVLDEIDAALDNTNI *F GKVASYIRDHTTNLQTIVISLKEEFYGHADALVGITPGVRTQLV *F >AE003746|GENSCAN_predicted_CDS_11|3735_bp *F atgaccgaagaggacgacgatgtggcccaaagggtggcgacggcgcccgtccgcaagccg *F gacgacgagacggccttcctggagtacatcgaaatggagaacttcaagtcctaccgcggt *F cacatagttgtgggtcctctgaagcaattcaacgctgttattgggcccaacggatcaggc *F aagtcaaacttcatggatgccatcagtttcgtgatgggcgagaagaccagcagtttgcga *F gtaaagcgcctgaacgacctcatccatggctcctccattggcaagcccgtttcccgcagt *F tgctacgtgaccgccaaatttgtgctgaacgaggagcgccacatggacttccagagggcg *F gttatcggcggctcctcggaatatcgtatcaatggagagagcgtatcgagcagcacgtac *F ttgaacaagctggagaaaattggcatcaatgttaaggccaagaactttttagtattccag *F ggagctgttgagaacatagccatgaagacacccaaggaacgcactgctctatttgaggaa *F attagcggttctggcctgcttaaggacgactataatcgacttaaacaagaaatgattgtt *F gccgaggaggagacccagtttacttaccaaaagaagaagggcatcgcggcggaaagaaaa *F gaagccaagcacgagaagatggaagctgatcgctacactcgcctgcaaaatgaatacaat *F gaaaaacaagtggagtatcaattatttaggctattccacgtggagagggacatccggaag *F tttacgagcgatttggaagtgaggcaacaggaagttaaagcagtcgaacagcgaaaagaa *F gctgccgatgaaatcctgcgcgaaaagaagaaagacgccggaaaaatcacccgagacctg *F gccaaaatcgatcaggaaatcagagagtttgaaacccagatgaacaaacgcaggcccctt *F tacatcaaggccaaagagaaagttacccactgcaagaagaagctcatttccctacagaag *F actctggaaacagccagggaggcggacaatgcccatcagtcggacatacggaaactggag *F aagcagctggctgatgtggaggccctgaaaaaacgtttcgaggacgagatcgagaacgag *F tcgcagcgccgcggcaaaagcgttaacatggaagagggccttgtgcaagagtacgatcgc *F ctgaagcaggaggcggaagccacagctacgcagtaccgttcagagctggactcagtaaac *F cgggagcaaaaatccgaacaggacacgcttgatggggagacaaatcgtcgcgcctccgta *F gaggagtccttcaagaagctcacattacagcgcgaggaagctgtaaagcgtcgagacaaa *F ctgatggatcacatcaaatcatcacaggctgccttggaggaacagaaccggatcaaggac *F gagctccggcgagatgttggcacatccaaggagaagatagccgaaaagcaacgcgaattg *F gaggacgtacgcgatcaactgggcgatgccaagagcgacaagcacgaggatgctcgtagg *F aaaaagaagcaggaggtggtggagctcttcaaaaaacaggttcccggagtgtacgaccgt *F atgattaacatgtgtcagccgacgcataaacgctacaatgtggccgtcaccaaagttttg *F ggcaagttcatggaagccattattgtggacaccgaaaagacggctagacactgtattcag *F atcttgaaggagcaaatgctggaagtggaaaccttcttgcccttggactatctgcaagtt *F aagcctctgaaggaacgacttcgtaacatcagcgacccgcggaacgtgcgattggttttc *F gatgtactaaagtttgagcctcaagagatcgaacgggctgtgctcttcgccacaggcaat *F gctctcgtttgcgagactcccgaggacgccatgaaagtggcttacgagatagaccgatca *F cgtttcgatgctctggccctggacggaacattctaccagaaatcgggtctcatatctggc *F ggtagtcacgatctggctcgcaaagctaagcgatgggacgagaagcacatggctcagctg *F aaaatgcagaaggagcgccttcaagaagagctcaaggagctggtgaaaaagtcacgtaag *F cagagtgagctcgccactgtggagtcgcagatcaagggtcttgaaaacaggcttaaatat *F agtatggtcgatttggagtcctccaagaagtcaattagtcagtatgacaatcagttgcag *F caagtccaatcgcagttggacgaatttggacccaagatccttgagatcgagcgtcgtatg *F caaaaccgcgaggagcacatccaggaaatcaaagaaaacatgaacaatgtggaggacaaa *F gtatatgcctctttctgccgtcgcttgggcgtgaagaacatacgacaatacgaggagcgt *F gagctcgtcatgcagcaagagcgagcgcgtaaacgggctgagttcgagcagcagattgat *F tccataaactcgcagctggacttcgagaaacagaaagacactaaaaaaaacgttgagcgc *F tgggagcgcagcgtgcaggatgaggaagatgccctagagggactcaaattggctgaggca *F cgctatttaaaggaaatcgacgaggataaggagaaaatggaaaaattcaagcaggacaag *F caggccaagaagcaagccgtggatgacatggaagaggacatatctaaagctcgcaaggat *F gtggcaaatttggccaaggagatccataacgtgggcagtcatttatctgcggtagagtct *F aagatcgaggccaagaaaaacgaacgtcagaacatactgttgcaagcaaaaaccgattgc *F atcgtggtaccactgttgcgcggctcgctggacgacgcagtgcgacaaagcgatccggat *F gtcccatccacctctgcagcgatggaaaatattattgaagtggattattcatctctacca *F cgagagtataccaagctgaaggatgactccgctttcaaaaagacccacgaaatgcttcaa *F aaggacctgcaaagcaagctggacgtcttggagcgcatacagacacccaacatgaaagca *F ctgcagaaactcgatgctgtaacggaaaaagtgcaatccaccaatgaggagtttgagaat *F gcgcgcaagaaagcaaagagggccaaggcggcatttgaacgggttaagaacgaacgctcc *F tcgcgattcgttgcgtgttgccagcacatatcggacgccattgacggcatctacaagaaa *F ttggctcgcaacgaggcagcccaggcttatatcggtcccgacaatcctgaggaaccatat *F ctggatggtattaactataattgtgtggcgccaggcaaacgtttccaacccatgaacaac *F ttgagtggtggcgaaaaaacaatagcagccttggctctgctcttctcgacccacaggtat *F gcagatataatatttttccacattttgagagtaaatattaatatatttttatgcagcttt *F catccagcgccgttctttgtccttgacgagattgatgccgccttggacaacacgaatatt *F ggcaaagtcgcttcgtatataagggatcacacaaccaacctgcaaaccatcgtcatttcc *F ctgaaggaagagttctatggtcatgctgatgctcttgtgggcattacgcctggggtacgt *F actcaactggtgtaa *F >AE003746|GENSCAN_predicted_peptide_12|97_aa *F MCTDDDNLDDDDDVDDDDEDNEDSLSVWHRTATDMTSTTRIRDTLRRILNIPLTTALEYK *F IHCDSLKYVSMPRRQNHKLGNLFYRNRYGFNSRYGKS *F >AE003746|GENSCAN_predicted_CDS_12|294_bp *F atgtgtactgatgacgacaatctggatgatgatgatgatgttgatgatgatgatgaggac *F aacgaggatagcttatcagtatggcaccggactgccactgatatgaccagtacaacacgt *F atccgtgatacgttacgcaggatattaaatatacctctaacaacggcattggagtataag *F atacactgtgatagccttaaatatgtttcaatgccgagacgtcaaaatcataagctggga *F aaccttttttatcgaaatcgatacggctttaattcaagatacggaaaatcatga *F >AE003746|GENSCAN_predicted_peptide_13|146_aa *F MFYRRSNTVETKKILEKLRLYRTRLVQIHEYHHFNRVISSKVNSTLQLSIHSEERRSAQP *F TSTLFRSYKMKMEDVSVEKRVRKTNRKSGVLLCGVRSSRSNWRLHTERLCQQLNQPQNHK *F KYPHFRLFLLTASNASILQIAVMRFS *F >AE003746|GENSCAN_predicted_CDS_13|441_bp *F atgttttatcgacgcagcaacacagtagagacaaagaaaatcttggaaaaactgcgacta *F tatcgcacgcgtttggtgcaaatccatgaataccaccattttaaccgagtaatatcatca *F aaagtgaactcgacattgcagctttccattcattcagaagaaagacgttccgcgcagcca *F actagcactttgttccgcagctacaagatgaaaatggaagacgtcagcgtggaaaagcga *F gtgaggaaaactaacaggaaaagcggagttttgttgtgcggtgtgcgtagcagcagaagc *F aactggcgactgcacactgagaggttatgccagcaattgaatcagccacagaaccataag *F aaatacccgcactttcgtctctttcttctgactgcttctaacgcttctatccttcagatt *F gccgttatgcggtttagttaa *F >AE003746|GENSCAN_predicted_peptide_14|450_aa *F MYSRYRKNWPDIVDSDDSDVDNQIDVDNLPPLEVGPGENRLQHTYCLWFSRKETQRAAAD *F YSKSLHMVGRCASVQQWWSLYSHLIRPTALKPYRELLLFKQGIIPMWEDPANSKGGQWLI *F RLRKNKVDRAWENVCMAMLGEQFLVGDEICGVVLQTKYPMTKDRLAALHAAQSDDEEETE *F VAVNVDGHDSYMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDL *F MADIKKNANRVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRTQ *F TDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIEAR *F HQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKALKYQ *F SKARRKKIMILICLTVLGILAASYVSSYFM *F >AE003746|GENSCAN_predicted_CDS_14|1353_bp *F atgtactcgcgttacaggaaaaactggccagatattgtcgacagcgacgacagcgatgtg *F gataatcagatagatgtggacaacctgccaccactggaggtgggtcccggcgagaaccgg *F ctgcagcacacatactgcctctggttctctcgcaaggagacgcagcgcgcggccgccgac *F tacagcaagtcgctgcacatggtcggccggtgcgccagcgtgcagcagtggtggtcgctc *F tactcgcacctcatccggcccaccgccctgaagccctaccgggagctcctcctcttcaag *F cagggcatcataccgatgtgggaggacccggcgaacagcaagggcggccagtggttgata *F cgactacgcaagaacaaggtcgaccgggcctgggagaacgtttgtatggcgatgctcggg *F gagcagttcctcgtcggcgacgagatatgcggagtcgtgctacagacgaaatatccgatg *F actaaagacagattagccgctctccatgccgcccaatccgatgacgaggaggagacggag *F gtggccgtcaatgtggatggccatgattcctacatggacgacttcttcgcccaggtggag *F gagatccgcgggatgatcgacaaggtgcaggataacgtcgaggaggtaaagaagaagcac *F tcggccatcctgtccgccccacaaacggacgagaagaccaagcaagagctggaggatctg *F atggccgacatcaagaagaatgccaatcgcgttcgcggcaagctcaagggcatcgagcag *F aacatcgagcaggaagagcagcagaataagtcgtcggcggatctgagaattcggaagacg *F cagcactcgacgttgtcacggaaattcgtcgaagtgatgaccgagtacaatcgcacgcag *F accgactaccgagagcgctgcaagggaaggatacagcgtcaactggagattaccggacga *F ccgaccaacgacgatgagctggagaagatgctggaggagggcaactcgtctgtgttcacg *F cagggcatcatcatggagacgcagcaggccaaacagacgctggcggacattgaggcccgc *F caccaggacatcatgaagctggagacatcgatcaaggagctgcacgacatgttcatggac *F atggccatgctggtggagtcgcagggcgagatgatcgatcgcattgagtaccatgtggag *F cacgctatggactatgtgcagacggcaactcaggacaccaagaaggcgctcaagtaccag *F agtaaagcccgacgaaagaagatcatgatactgatctgcctcactgtgctgggcatctta *F gcggcctcatatgttagcagttatttcatgtaa *F >AE003746|GENSCAN_predicted_peptide_15|465_aa *F MKLSIRMLDQRTITLEMNESQEVRALKQKLGNLPEVAMPAENLQLIYSGRIMEDAMPLSE *F YRIAEDKIIVLMGKKKVDKSSPEEKVAPTPPLAAGPNVLRTEDVVPSLAPNDQWVSDLMS *F MGYGEEEVRSALRASFNHPERAIEYLINGIPQEVVSEQGLAAIPSVQTSDQLQQLMADLN *F ITRMREMINQNPELIHRLMNRLAETDPATFEVFQRNQEELMNMISGGASRTPNEIEHLQI *F TLTAEETAAVGRLEALGFERVMAVQAYLACDKDEQLAAEPLFRGAVRYGSAALSECSNTA *F DTTYPTFGYRVSEYRIFAVILQKSVCLRSPASRIMGKSEKKSKKKHKEKRREHKEKHKSK *F KSKKHNRKKEESPPIASPTPIQPERVNQEEEDDFAIPIEEYQRRQSQIRKEVDPVTGRVR *F LIKGDSEVLEEIVTKERHLEINKKATRGDGEFYEARSLDAAKRRK *F >AE003746|GENSCAN_predicted_CDS_15|1398_bp *F atgaagctgtctatacgcatgctggaccaacgcaccatcactttggagatgaacgaatcg *F caggaggtgagggctctgaagcagaaattgggcaatttacccgaagtcgccatgcccgcg *F gagaaccttcagctgatatacagtggccgcattatggaggatgccatgcccctcagtgaa *F tatcgtatagccgaggacaagatcattgtgttgatgggtaagaagaaggttgataagagc *F tcgccagaggagaaggttgccccgacaccaccgttggccgctggcccaaatgttttgcgc *F acagaggatgtggtgccttcactagctcccaatgatcagtgggtgagcgatctcatgtca *F atgggatatggcgaagaggaggtacgctcagccctccgggcgagctttaatcatccggaa *F agggctatagagtatttgattaatgggattcctcaggaggttgtttcagagcagggatta *F gctgcaatcccgagcgtacagacaagtgatcaattgcagcaattaatggcagatcttaac *F attacacggatgcgtgagatgattaatcagaatccagaactaatacacagactaatgaac *F agactggctgaaaccgatccggctaccttcgaagtctttcagcgtaaccaggaggagtta *F atgaacatgatttcaggcggcgcaagtcgcaccccgaacgagattgaacatttacagatt *F actttaaccgccgaagaaaccgccgccgtagggcgtttggaggcactgggtttcgaacgt *F gtgatggccgttcaggcctatctggcctgcgacaaggacgagcagctggccgcagagccg *F ctttttcggggagcggttcgctacgggtccgccgctctctctgaatgcagcaacaccgct *F gacacaacataccccacatttggttacagggtatcagagtatcgaatttttgctgtcatt *F cttcaaaaatcagtttgtttacgttcacctgcaagccgcataatgggaaaaagtgagaaa *F aaatcaaagaaaaagcataaggagaagcgcagggaacacaaggaaaagcacaagtcaaag *F aaatccaagaaacacaaccgaaaaaaggaagaatctccgccgatagcttcaccaacacca *F atacagcctgaaagggtcaaccaggaggaggaggatgactttgccataccaatagaggaa *F taccagcgccgtcagagccagatccgcaaagaggtggacccggtgacgggtcgcgtccgg *F ctcatcaaaggcgacagtgaggtgctggaagagattgtgaccaaagagcgccacctggag *F atcaacaagaaggccacccgcggtgatggagagttctacgaagccagatctctggacgcc *F gccaagcgtcgcaaatag *F >AE003746|GENSCAN_predicted_peptide_16|669_aa *F MNNSPKDPYRGGGGGSGPQDGAGSTGVSINIESDDNDSTTAEHDYLLPASSSSVTSGGGV *F GGGGVVSGGSTSTSVNLDHQHVPTARSSSLSGGNVRHGNILSTFLARQRSYTPASNSSSP *F VRVTTQMNRRLQQSRSMGANTGGSLEEPSSAGAAGGPGASPGTAAGVRQMGFWRVNLNDV *F FSLSTASSTEALRSFISHSNFRYPPATTAAPAAPGPQPAANPVDNSHILLGQQQPSMPEP *F VSPLRYGRVSAVSGASSAGAMGRSISLREGEMRHNHSLNGGRHNASAIGLNSNPAAFDER *F EANESQDLDESDGNVENGGGGGNPPEPAENNPEDEHLISDDMVVQILSHFVRYLPLIFIL *F FFKFLHDHLLGIVDLLVLQTVMYNVNRSVRNQVARLAQKNYAVMVRDTFLVAVVVTVRLF *F LATSPPDPFGLIVPPSRKSVFIEVTALPFHTSSEGDSPTTSSEPIKTNMYKDTYKSLNVI *F PLGMLLYYIAVSDLIIKLLTMLVKLIITMLPHHLMRLKVRARLYVLVEYISQFYRAMTPI *F TQWLLFLYESYSGLEVVSGGLFSAMYLGAKIFELVERGKSLKKAIVTFRKNIDSERPPTK *F DELDAAGALCPICHDAFNTPTVLECGHIFCDECVQTWFKREQTCPMCRAKVSDDPAWQDG *F STTFFHQLY *F >AE003746|GENSCAN_predicted_CDS_16|2010_bp *F atgaacaacagccccaaggatccctatcgtggaggaggaggaggttctggaccccaggat *F ggcgctggatcgaccggtgtgtctatcaacatcgagagcgacgacaatgactcgacgacg *F gcggaacacgactacctgctgcctgcctcctcatcttcggtgacctctggtggtggcgtc *F ggaggtggaggcgtcgtctctggaggatcgaccagcaccagcgtaaacctggaccaccaa *F cacgttcccacggcgcggagcagcagccttagtggtggtaatgtccgtcatggtaacatt *F ctatccacatttttggcccgtcagcgctcgtacacgccagccagcaacagcagttccccg *F gtgcgggtgaccacacagatgaaccgtcgtcttcagcagtcccgtagtatgggcgccaac *F accggtggcagtctcgaggagccgtccagcgccggagcagcaggtggaccaggagcatcg *F ccaggaactgcggcaggtgttcgacaaatgggcttttggcgtgtcaacctcaacgatgtc *F ttttcgctttcaactgcctcgtcgacagaggcgctccgatccttcatctcacattccaat *F tttagatatccacctgcaaccactgcagcccccgcggcaccaggacctcagccagccgcc *F aatcccgtagacaactcgcacatactgctcggccagcagcaaccatcaatgcctgagcca *F gtcagccctctgcgatacgggcgcgtttcggccgtatctggagccagttcagctggagct *F atgggtagaagtatctcgctgcgtgagggtgagatgcgacacaatcacagccttaacgga *F ggacggcacaatgccagtgccataggactaaacagcaatccggcggccttcgacgaacgc *F gaagcaaatgagagccaagaccttgacgaaagcgatggcaacgtggagaacggaggtgga *F ggtggcaatccaccagagccagcagagaataatccagaggatgaacatctcatctcggat *F gacatggtggtccagattctcagccattttgtgcgatatctgccgcttatctttatactg *F tttttcaagtttttacacgaccacctgctgggcattgtggatcttctggtgctgcagact *F gtgatgtataatgttaaccgatcagtacgcaatcaggtagcccgtctagctcagaagaac *F tacgccgtgatggtgcgggatactttcctcgttgccgtggtggttacagtgcgtctattt *F ttggccacttcgccgccagatccgtttggactgattgtgccgccttccagaaaatccgtt *F ttcatagaggtcacagcgctgccttttcacactagctcggaaggcgacagccccacaaca *F tcgtccgaacccatcaagaccaacatgtacaaagatacgtacaaatctctaaatgtgatc *F ccactgggcatgttgctgtactacattgctgtgagcgatctcattatcaaactgctgaca *F atgctggtcaaacttattattaccatgctgccgcaccacttgatgagacttaaagtgcgg *F gctaggctttatgtgttggtggagtacatttcgcagttctaccgggcaatgacacccatt *F acccagtggctcctatttctatacgagtcctactccggcctggaggtggtttccggagga *F ctcttttccgcaatgtacctgggtgccaagatatttgagctggtggagcgcggcaagtcg *F ctaaagaaagccattgtgaccttcagaaaaaatattgactctgagcggccgccgaccaaa *F gacgagctggatgctgcgggtgccctgtgtccaatctgccatgacgccttcaacacaccc *F actgtgttggagtgcggccacattttctgtgatgagtgcgtccagacctggttcaagcgc *F gaacagacctgtccgatgtgcagggcgaaggttagcgatgatcccgcctggcaggacggc *F agcactaccttcttccatcagttgtactag *F >AE003746|GENSCAN_predicted_peptide_17|546_aa *F MSQTLDDLLLRQEHARQLRQATRSKFRRINSLDLIPEHPSQSEEEEDKESQTAHKHFVTL *F RRQRTADGNLLRHQFQTQSPPQPNLGTRVLLFGPQLLMRLVLTILRYVLYIPLSIAAPSF *F WLSALLWIFWKLLRVPIALVKWLLSGEEELGAVQRQKTILLSCGSTIQTLHLARNFYGSG *F ARVVVFEFEGLFGLARFSTAVDKFYVVPRPTASNPDQYIAALCHIVKKERPSVYIPVCAT *F SPAYYDSLARPHLEVLGCASFIPGVQETLQLDDCLQLFQRCEQQQMALPAHVVLTAPRQL *F QQIYESGFVGSYRNILMAAGMQGVLERHKYILPNRRAELKLNQHDISERQPWLVVRDQPG *F YHHYVTCTTVKDSRVVANVSCRVEHHTKNLIPVPRDDEAQIELWLRSFFAKVRFQRPING *F HISFRLVKSPAHGGQFVPLGTRLGVALPYICHNRSHAQLLCRAMKCIHRRGLPEDELPNW *F SWSALERTTSTTALDKREALFAYWDPLPYCAYYHFQLPLKNVKLFLQRRNRSATKTLSPR *F ITVPVH *F >AE003746|GENSCAN_predicted_CDS_17|1641_bp *F atgtcccagaccctggacgatctgctgttgcgccaggagcacgcccggcaattgcgccag *F gccacgcgctccaagttccggcgcatcaattcgctggatctaataccggagcatcccagc *F caatcggaggaggaggaggacaaggaatcccagacggcccacaagcactttgtcaccctg *F agacgacagcggacggctgatggcaatctgctgaggcatcagtttcagacgcagtcgccg *F ccacaacccaatttaggcacacgggtcctgctcttcggaccacaactgctgatgcgtctg *F gtgctgaccatcttgagatacgtcctctacataccactgtccattgcggcgccaagcttt *F tggttgtccgccctgctctggatcttttggaaactgctgcgggtacccattgcgttggtc *F aagtggctactgagcggcgaggaggagctgggtgcagtgcagcgacagaagaccatactc *F ctcagctgcggcagcaccatacagaccctacatttggccaggaacttctacggatccggc *F gcccgtgtggtggtctttgagttcgagggtttgttcggattggccagattttccacagcg *F gtggacaaattctatgtggtgccacggccaacggccagtaatccggatcagtacatagcc *F gccctgtgccacatcgtgaagaaggaacgaccctcggtctatatacccgtttgtgccacc *F agtccggcatactatgattccctggcccgaccacatctggaggtcctgggctgtgccagt *F ttcatacccggcgtgcaggagacactgcagctggatgactgcctccagctctttcagaga *F tgcgaacagcaacagatggctctgccagcccatgtggtgctgacagctccacggcaattg *F cagcagatctacgagagcggtttcgtgggcagctataggaacatactcatggccgcaggg *F atgcagggagtcctggagcggcacaagtacatactgcccaataggcgggcggaactaaag *F ctgaatcagcacgatatcagcgagcggcaaccgtggctagtggtaagggatcagccgggc *F tatcaccactatgtgacctgcaccaccgtcaaggattcacgggtggtggccaatgtgagt *F tgtcgggttgagcatcataccaagaatctgatcccagtgcccagggacgatgaggcgcag *F atagaactctggttgcgctccttctttgccaaggtgcgcttccagaggcccatcaatggg *F catataagcttccgcctggtcaagagccccgcccatggtggtcagttcgtgcctctgggc *F acgcgactgggcgtggccctgccctacatatgccacaatcgatcgcatgcccagttgctg *F tgccgtgcgatgaagtgcatccacaggcggggactgcccgaggatgaactgcccaactgg *F agctggtcggcgctggagagaaccacttcgacgacggcgctggacaagcgggaggcattg *F tttgcctactgggatccactaccctattgcgcatactatcatttccagctgcccctgaag *F aacgtgaagctgtttttgcagcgccggaatcgcagcgcgaccaaaaccttatcgccacgc *F atcacggtgccggttcattga *F >AE003746|GENSCAN_predicted_peptide_18|2084_aa *F MFIVANSRDDPLSSVRLRFLAIGYKSSSPFEGSSEAEVAPVAKRAGKSPSEPKISIMQAY *F RDNFKQTPCPSAVDLQAAGPAHQSATASRLPFSRSQRGRDPSASGASVSASVAGGLTPRM *F MSPGPPGAGGGGGGGVSGGGSGDPSALLRQNQELRQRLADESHSYRRRLDTYKQAQHNQA *F NLVSRLQSKIQQYRQRCSDLEERMHETIKPTAGVGPKLTTGPTNQVLCSTSLTLGQSSLP *F CSSSLDSPPPSCSRDYVDDVLVTGGGAGAAELCRKLEEEHQRCEQIVAQNSALRQQLEES *F NRTNEALTNDLQKLTNDWASLRDELLIKEDEFKEEEQAFKDYYNSEHNRLLKMWREVVAV *F KRSFKEMQTAMKAEVAKMGQEINCVGKDINGSNATVAFAVQQAKRAADDELKQSQRSNDE *F LQNQLATLKVQYESARHEIMERDQRLLELMNQLKKLEDRCAQAESQAALASRYSDEIERL *F NNSMREIAQAVVQDAENADREADAEVTGGVMQHMHLTRDAASVVGGAGGAGSTAGGGGKS *F PRRNSTRASQAFAEGTISAVQAALHKYQLALHDMQVKFQNTSETLRTTKAQLETSEGTKQ *F LLTTKMQQLTEKLDSSNSKLSELLQERESLQRGLDDIRVQKQQSEMGRADINSAFENLSS *F DYEKMQLNCGKLQKRIDSMEEDKKAVELEIQRILKDKNITELNLRSEEDRSSRLREETIS *F LREELNRVSLNRDLLEQQRIESDNLINLLEKQKSDLEYDLDKLLLEKCDLQEKHEKLSNN *F SCSTSDELKSVQNCLQEAQEERKKLRIQSVDQCNEIGELKKELAILDKARLELETDNLSA *F GEKLKCLQLEKEKILQDLACVTRDRGDIHNQLTAMCRKKEALNEELMRTRQRLEQTTETN *F SRLNRNLEEMVKDVEEKQVVIDLHEKDTHRLNELLAALRSEKESLESVLFDTNTSLEATE *F ERRSQLERDLQEALVREESLKNHVARLQKELEQCQRKAQETKTQLLNAARAAESDFNQKI *F ANLQACAEEAAKRHGEEILQLRNALEKRMQQALQALQTAKDDEIEKLQERLATLQAHLES *F LVQQHEEALIRAESEKQQALLIAHRDKQAVAERLEAVSRDLKTEQESLDRSRREANARDE *F KQRAAIAQLKDEMVQMRTKEEEHKIKLEECIRKQELQLSSLREERESLCRVSEELKMEIR *F LKEDRMESTNNELQDALRKSKEGEGFIDSLRKELTDCRRQLADSNIERDKYSGSNKELRD *F HVKRVESAKREQARAIEEALQKISNLEDTKNSLENERTRLSTILKETENHFTKTTQDLNA *F TKAQLQKAQVEFAQKDEGGKELQCKLVAEVELKERAQQELCQIKKQLSDLEANLCATRQE *F LGRARCQNNQEEHRFHAREQELAQRLEEGRGREKRLEDQKHNLEVCLADATQQIQELKAR *F LGGAEGRIRALDEQLSCVELHKRDTEQKLSSVVHTLRRIAGIQVDGSVNLSHRLLSPSRR *F FSPSRSCGDYDNRSTSQCPDGPIDVDPDLVRKGVRNLMHQVAQLEREKDDYKSQLGAAKK *F QLQDAADQQLRCDAKLGKLQAMLRNLQEEKSNLETDRKMKISAIQALEEKLKHRNDECQM *F LRERLAQTEMQLAATSEENGQNEERLEKSRQQCSKLDNEKRQLQEELAKVEGRASKLELQ *F RVAMEGDLTRLQMALQEKDCSIRQMAERLENQNRALTQLEDRCTALKSTVDQLKERLQKS *F AVSETQLRGEIKTLQKELSEQGHCSQANEDKLKLVQKSLQTAENEKRILTERLDSAQTNL *F NELRRSQQAQLDGNQRLQEQVTDLEVQRSALESQLRIAKWNQESGGDKDLTNGNGGGNGE *F EELSRQLKSSQREKSELRSKLQTLQDKVKQLECDRKSKFSGGNAYDRAEKSNSFYGGAAE *F SGEFDSNRYDVGGGNAGGGSFNCGLDHSVIEQETRDLRLKVRRLETLLAEKESELARCKA *F RMNDSAKCHDGLDGDRYRSAQMHAEKLLDAREQSHRQQVLRLENQISMLREQLAQEAKRR *F QQYILRSSKANREMQHLRSTLGDSLRNVSQHPVDPHLLESESRR *F >AE003746|GENSCAN_predicted_CDS_18|6255_bp *F atgtttattgtcgccaactctcgggatgatccgctctcctccgttcgtctgagatttttg *F gcgatcgggtataaatcatcgtcgccgtttgaaggttccagcgaggcggaggtagcgccc *F gttgccaagagagccgggaaatctccgtcggagccgaagatcagtataatgcaggcgtat *F cgcgataacttcaagcaaacgccctgcccatcggccgtcgaccttcaggcggcgggacca *F gcccaccaatcggccaccgcctctcggctgcccttctcgcgcagccagcgtgggcgtgac *F ccctcggcaagtggcgcctcagtttccgcctccgttgctggtggtctaacgcccaggatg *F atgagtccgggtccgccgggcgccggaggaggaggaggaggaggagtgagcggcggcggc *F agtggagatccctcggccctgttgcgccagaatcaggagctgcgccaacggctggccgac *F gagtcgcatagctatcgacgccgcctggacacctacaagcaggcgcagcacaaccaggcc *F aacttggtcagccggctgcagtcgaagatccagcagtatcgacagcggtgtagcgacctg *F gaggagcgcatgcacgagaccattaagccgacggcgggagtaggacccaagctgaccacg *F ggtcccaccaaccaagtgctgtgttcaacttctttaactctgggacagagcagcttgccc *F tgcagctcctcactggactcgccgccgcccagttgcagtcgtgactatgttgacgatgtc *F ttggtcaccggaggcggcgccggtgcggcggaactgtgccgcaaactggaggaggagcac *F cagcgctgcgagcagattgtcgcccagaacagcgcactgcgtcagcagctggaggagtcg *F aatcgcaccaacgaggcgctcaccaacgacctacagaagctgaccaacgactgggcgagt *F ctgcgggatgagctgctgatcaaggaggatgagttcaaggaggaggagcaggccttcaag *F gactactacaacagcgagcacaatcgcctgctgaagatgtggcgcgaagttgtggccgtc *F aagagatccttcaaggagatgcagacggccatgaaggcggaggtagccaagatgggtcag *F gagatcaattgtgtgggcaaggacatcaatggctccaacgcaacggtcgcctttgccgtc *F cagcaagccaagcgggctgcggatgatgaactgaagcaatcgcagcgcagcaacgatgaa *F ctccagaatcaattggccaccctgaaggtgcagtacgagagtgcccggcacgagatcatg *F gagcgggatcagcgactactggagctaatgaatcagttgaagaagctggaggatcgctgc *F gcccaagccgaatcccaagcagctctggccagtcgctatagcgacgagatcgagcgactg *F aacaattccatgcgagaaatcgcgcaggccgtcgttcaagatgctgagaacgcagatcgc *F gaagcagacgccgaggtcaccggcggtgtcatgcagcacatgcacctcacgcgtgacgcc *F gcctctgttgtgggcggagcaggtggagcgggcagcaccgccggcggcggagggaaatca *F ccgcgtcgcaactcgacacgcgcctctcaagccttcgccgagggcaccatctcagccgtc *F caggcggcgctccacaaataccagctggccctgcacgacatgcaggtgaaattccagaac *F accagcgagaccctgcgcaccaccaaggcccagctggagaccagcgagggtaccaaacag *F ctgctgaccaccaagatgcagcagctcaccgagaaactggacagcagcaactccaagcta *F tcggaattgctgcaggaaagggagagtctgcagcgcggactggacgatatccgtgtccag *F aagcagcagtctgagatgggacgagccgatatcaatagtgcgttcgagaatctgagcagt *F gattatgagaagatgcagctgaactgtggtaaactccaaaaacgtatcgattccatggag *F gaggacaaaaaggcagtggagctggagatccaacgtatactgaaggacaagaacataacc *F gagttgaatttgaggtctgaggaagatcgcagtagtcgtttgcgggaggaaaccatatcc *F ttgcgcgaggagcttaaccgagtgagcctgaatcgtgatcttctcgagcagcagcgcatc *F gagtccgataatttgatcaatctgctcgagaaacagaagtccgacctggagtacgatctg *F gacaagctgttgctggagaagtgcgatctgcaggagaagcacgagaagctatccaacaat *F agctgctccaccagtgatgagctgaagagcgttcaaaattgccttcaggaggcgcaggag *F gagcgcaagaagctccgtattcagtccgtcgatcagtgcaatgaaatcggagagcttaag *F aaggagctggcgatactggacaaggcacgactcgaactggagacggacaatctgtcggct *F ggggaaaagctcaagtgtctgcagctggagaaggagaaaattctgcaagacttggcctgc *F gtcacccgggatcgtggtgacatccacaatcagctaacggcgatgtgtcgcaaaaaggag *F gctctgaatgaggaacttatgcggactcggcagcgtctggagcaaaccaccgagaccaat *F agccggctgaatagaaatctggaggagatggtgaaggatgtggaggagaagcaagtggtc *F atcgatctgcacgagaaggacacacatcgcttgaacgaactcctggccgccctgcgttcg *F gagaaggaatccctggaatcggtgctcttcgatacaaacacctcactggaggccaccgag *F gagcgacgcagtcagctggagcgggatctgcaggaggctctggtgcgtgaggagagccta *F aagaatcatgtggctcgcttgcaaaaggagctggagcagtgtcagcgcaaggcccaagag *F accaagacgcagctgcttaacgccgcccgtgcggctgagagtgacttcaaccagaaaatc *F gccaatctgcaggcttgtgcagaggaggcggccaaacgacatggcgaggagattctacag *F ttgcgaaatgccttggagaagcgaatgcaacaggctctgcaagcgttgcagacggccaag *F gatgatgagatcgagaagttgcaggagcgtctggccaccttgcaggcgcatctcgagagc *F cttgtccagcagcatgaggaggcactgattcgggcggagagcgagaagcagcaagccctt *F ttgattgcccaccgggataagcaagcggtggccgagcgtttggaggccgtatcccgggat *F ctcaagaccgaacaggagtccctcgaccggagcagacgggaggccaatgcgcgcgatgag *F aagcagagggctgccattgcccagctgaaggacgagatggtgcagatgcgcaccaaggag *F gaggagcacaagattaagttggaggaatgcatccggaagcaggagctgcagttgagcagc *F ttgcgcgaagaacgcgaatccttgtgccgtgtgagtgaggaactaaagatggagattcgt *F ctgaaggaggacagaatggagagcaccaacaatgagttgcaagatgcgctgcgcaagtcc *F aaggagggtgagggcttcatcgatagcctgcgcaaagagttgaccgactgtcgccgccaa *F ctggcggacagcaacatcgagcgggacaagtattccggcagcaacaaggagctgcgcgac *F cacgtcaagcgtgtggagagcgccaagcgggagcaggcgcgcgccatcgaggaggctctg *F cagaagatcagcaatctggaggataccaagaactcgttggagaacgaacgcactcgattg *F agcaccatactgaaggagacggagaatcactttacaaagaccacccaggatctgaatgct *F accaaggcgcagctgcagaaggctcaagtggagttcgcccagaaggacgagggcggcaag *F gagttgcagtgcaagctggtcgccgaggtggaattgaaggagcgggcacagcaggagctc *F tgccagattaagaagcaattatcggatctggaagccaatctgtgtgccactcgccaggaa *F ttgggcagggctcggtgtcagaacaaccaggaggagcatcgcttccatgccagggagcag *F gagttggcccagcgcttggaggagggtcgtggtagggagaagcgcctggaggatcagaag *F cacaacctggaggtctgcctagccgatgccactcagcagatccaggagttgaaggcccgt *F ttgggcggcgccgaaggtcgcatccgtgctttggatgagcagttgtcctgcgtggaacta *F cacaagcgggataccgaacagaagctatcctcggtggttcacactctgcgccggattgct *F ggcatccaagtggacggcagtgtaaatttgtctcatcgcttgctgagtccctcgcgaaga *F ttcagtccgtctcgcagctgcggagactatgacaacaggagcacatcacaatgcccagat *F ggaccaattgatgtggacccggatctggttaggaagggtgtccgcaacctgatgcatcag *F gtggctcagttggagcgcgagaaggatgactacaaatcccaattgggagcagctaagaag *F caactccaagatgctgctgaccagcagctacgatgcgatgccaagctgggcaaactgcag *F gccatgctaagaaatctccaagaggagaagagcaatctggaaacggaccgcaagatgaaa *F atctccgccatccaggcgctagaggagaagctcaagcatcgcaacgatgagtgtcagatg *F ctaagagaacgtttggcccaaacagagatgcaactggctgccacatccgaggagaatggc *F cagaacgaggaacgactggagaagagccgacagcagtgctccaaactggacaatgagaag *F cgccagctgcaggaggaattggccaaggttgagggccgggccagcaaactcgaactgcag *F cgtgttgccatggagggcgatcttaccaggctacaaatggccctacaagagaaggactgc *F agcattcgtcaaatggctgagcggctggagaaccaaaaccgtgccttgacccaattggag *F gatcgctgcaccgcccttaagtccaccgtcgatcagctgaaggagcgcctccagaagtcc *F gctgtgagtgaaacccaactgcggggcgagataaagacgctccaaaaggagctttccgag *F cagggtcactgctcccaggccaacgaggataagctgaagttggtgcaaaagtctctgcag *F accgctgagaacgagaagcgcatcctcaccgagcgcctagatagcgctcagaccaatctc *F aacgagctgcgacgtagccaacaggcccagctggatggtaaccagcgcctgcaggaacag *F gtgaccgatctggaggttcagcgttcggcactggagtcccaacttcggattgccaagtgg *F aaccaggagagtggcggcgacaaggatctgacaaacggcaacggaggcggaaatggcgag *F gaggagctcagcaggcagctgaagtcctcgcagcgggaaaagtcggagctacgcagcaaa *F ctgcaaaccctacaggacaaagtcaaacagttggagtgcgaccggaaaagcaagttttcg *F ggtggaaacgcctatgatcgggctgagaagtccaactccttttacggtggcgcagctgaa *F tccggcgagttcgactccaatcgctacgatgtgggtggaggaaatgctggcggaggctct *F ttcaactgcggattggatcacagtgtaatcgagcaggagacgcgcgatctgcgactcaag *F gtgcgccgcctggagacattgttggcggagaaggagtccgagttggcgcgctgcaaggcg *F cgaatgaacgacagcgccaagtgccatgatggcttagatggagatcgttatcgcagtgct *F cagatgcacgcagagaagcttcttgacgccagggagcagtcgcatcggcaacaagtgctg *F cgcctggagaaccagatctcaatgctgcgcgagcagttggcccaggaggccaaacggcga *F cagcagtacattctacgcagctcgaaggccaatagagaaatgcagcatctgaggagcacc *F ctaggagattccctgcgcaatgtctcccagcacccggtggatccccatctcttggaaagc *F gagagccggcggtga *F >AE003746|GENSCAN_predicted_peptide_19|461_aa *F MSGSFGRNQNNISCFGATFCQFRCECYNRCKIMHYSIIRKLVTAPKLANVPKRKWKSVVG *F LEVHAQIASASKLFSGSGTSFGAPLNSSVAYFDASIPGTLPVLNRKCVESGIKTSLALGC *F RVNEVSMFDRKHYFYADLPNGYQITQQRAALANDGKMTFPVITPGKKVYYKTAKLLQLQL *F EQDSGKSLHDDYLKRSLVDLNRAGLPLMELVFAPDLETGEEAASLVKELILILRRLQTCS *F CKMEEGALRVDANISIHQEGDPLGVRTEVKNIGSVRSISQAITYEINRQLETVANGGVIT *F NETRNWDAENRRTVAMRDKEVLQDYRFMPEPNLPPLHVNLKPGSMSTEDLLSVAALSEEI *F PELPEDTRQRLVEQHNLNAETAIILVLIELHSLQQICSPDEIENLCQLAIANQAKAVEQY *F QKGKAKALFAIAGEVAKLSSQKANMKLVVQRLEKLLKPTNK *F >AE003746|GENSCAN_predicted_CDS_19|1386_bp *F atgtccggcagctttggccgcaatcaaaacaacatcagctgttttggcgcaactttttgc *F caattccggtgcgaatgttataatcgatgtaaaataatgcattactcaataatccgaaaa *F ctggtaacggcgccaaagctagccaacgttcccaaaaggaaatggaaaagcgttgtgggt *F ttggaggtgcacgcacagattgccagtgcgtccaaactgttttccggcagtggcacatcc *F tttggagcaccacttaactcttcggtggcgtattttgatgcctccataccgggaacattg *F ccagttctcaacagaaaatgtgtggaatccggcattaagacatcacttgctttgggatgt *F cgggtgaacgaagtgtccatgtttgaccgcaagcactacttctatgcagatttgcctaat *F ggctaccaaatcacgcagcagcgcgccgctttggccaatgatggaaaaatgactttcccc *F gtgataacaccaggcaaaaaagtttactacaagaccgccaaactactgcagttgcaattg *F gaacaggatagtggtaaatctctgcacgatgattatctcaaaaggagcctggttgacctt *F aatcgtgctggacttcctctaatggagctggtttttgcaccagatttagaaacgggcgaa *F gaagcggcatcgctggtcaaggaattaatactgatactaaggcgtctgcagacatgcagt *F tgtaaaatggaagagggcgccctgcgtgtggatgccaacatatccattcaccaagaaggc *F gatcccttgggagtccgcaccgaagtaaaaaacattggctcggttcgaagcatttcgcaa *F gcaattacgtatgaaattaatagacagctagaaactgtggctaatggcggtgtaattaca *F aatgagactcgcaactgggatgcggagaaccggcgcacagtggccatgcgtgacaaagag *F gtgctgcaggactacagattcatgccggagccgaatctaccaccccttcatgtaaaccta *F aagcctggatcaatgtcaacagaggatttactttcagtggctgctctaagcgaggaaatt *F ccagaattaccagaggacaccaggcaacgtttggtggagcagcacaacctgaatgcggaa *F actgccatcattttagtgctaatcgaactgcacagcctgcagcaaatctgcagtccggac *F gagatcgagaacctgtgccagctggctatcgccaaccaggccaaggcagtcgaacagtac *F cagaaaggcaaggcaaaagctttgttcgcgatcgcaggcgaagttgccaaactgtcgtcc *F caaaaggccaacatgaagctggtagtgcagcgtctggaaaagctgctaaaacccaccaat *F aagtaa *F >AE003746|GENSCAN_predicted_peptide_20|1139_aa *F MVIEKIIGDLESNMTLENEEAKRKLVELLSQSESSPVSVNMPPIPTYHFPTDKEQWVVKF *F MLDYFFTTGSQRILEVLVKAQAPHDGYIFDKLDDCLKQSQHRVQSLQVFCFIVRHHPTWL *F YKIEKHRLIKSVFKLMTHEKEIVPLMSALLCIITLLPIIPNSVPNFLNDLFEVFGHLASW *F KLQNSNKLPDEKLVHLQLGLQMLFHRLYGMYPCSFIAYLVEFIKRGNGGGIFQHTIKPLL *F NTVRVHPMLVTATPETEVNNTRWKEMEPHDVVMECANLSLPVLLPETSNEDGSYAYPMTP *F GYSRMTSNTSNTDYSYQLREFQQSRNVYTRFDSFASGDDVGPIWSPHNEIATTSSGIPLT *F PTTSFILPLQPAMNSQLMVGMTGSSPPEAAVEATPETTPLKDMRDIKQPGRAVNSHAVRA *F IFAVSQPSSPMRKDQQSQFSFPDVSREAEESSHSYLEVNRGTAYDRRLSQVIQDRHNVER *F SVNTPCPSSLPEINSDLSLVGGSVYPSVTQEVAAVCGECNETDRNLCSVGGLHMPTSRSM *F HQLAKKRRNRMASYSGNGSCADSRSSAAKKASWSTEAENPMRRTKSCSALSGMRQQHLEE *F NDDEADCSSQRQRGENGNTQKTGSRLQRSGRNLAISAPKDTARSCTHASTQTVEGLDSAP *F AQYENWLIELLLECKEQRIDYERNLLYPQDILDEYIKHAIKANESFDAEQGQLMCLQLEY *F ESYRRSIHAERNRRLMGRSRDKRSLEMERDRLREQLKNFDAKNKDLANKMDQAIRLANER *F QNIHQEELGEMRAKYQHELEEKKCLRQANDDLQTRLTSELARHKEMNYELESLRGQVFSL *F GTELQHTQQQADIGLQCKQELARLEAEFIIMGEVQVRCRDRLAEIDNFRARDEELQMLQE *F SSNLELKDLRHSLDEKTSQLESMKHKISDLQAQLANSEKAMTEQKRLLSTVKDEYEEKFK *F SVNKKYDVQKKIIMQMEEKLMMMMQQPQGTTGHNTCSPDTDRTGECKSCFAAKEYLLLHS *F PTDIASSIERNSPLSTSLASSESLSASLRSTELKNLHQLVDTPTIPDVLNSMAGGAQFED *F EVRPPAVDLASSASTASAINIVPHALDLPSTSGGIGHTLTHPHPHPHLHLQQQQQDQLQ *F >AE003746|GENSCAN_predicted_CDS_20|3420_bp *F atggtgattgagaagatcattggtgacctggagtccaacatgacgctggagaacgaggag *F gccaagcgcaagcttgtggagttgctatcccagagtgagtcctcgccagtttcagttaat *F atgccacctattccaacataccatttccccacagacaaggagcagtgggtggtaaagttc *F atgctggactacttctttacaactggatctcagcgcattttggaggtactggtcaaagcc *F caggcacctcacgatgggtacatctttgacaagctggacgactgcctaaagcagtcgcag *F caccgagtgcagagcctccaggtgttctgcttcattgtgcgccatcaccctacttggctg *F tacaagatcgagaaacaccggctgatcaaaagtgtttttaagcttatgacgcacgagaag *F gagatagttccgctgatgagcgccctgttgtgcataattactctgctgccgatcataccg *F aattctgtgcccaactttcttaacgatctgtttgaggtgttcgggcatttggcctcgtgg *F aagctgcagaatagcaataaactgccggacgagaagctcgtccacctgcagttgggtcta *F cagatgctatttcaccgcctgtacggcatgtatccgtgcagctttattgcctatttagtg *F gagttcatcaagcgaggcaacggcgggggcatcttccagcatacaatcaagccgctgttg *F aacactgtgcgagtgcatcccatgctggtgacggccacgccagagactgaggtaaacaat *F acgcgatggaaggagatggagccgcatgacgtggttatggagtgcgccaacctatcgctg *F cccgtcctcttgcccgagacgagcaacgaagacggcagctatgcgtatcccatgacgcca *F ggatacagtcgcatgacttcaaatacctcgaatacggactacagctatcagctgagggag *F tttcagcaatcgagaaatgtctacacccgcttcgattcgtttgcctcgggtgatgatgtg *F ggtccgatctggagtccgcataacgagattgccacgaccagtagcggcataccgcttaca *F cccaccacatcgtttattttgccactacaaccggctatgaactctcagcttatggttggc *F atgactggctcctcaccgcccgaagcagctgtggaggccacaccggaaaccaccccctta *F aaggatatgagggatatcaagcagccgggacgtgcggtcaattcgcatgctgtgagagct *F atcttcgccgtaagccagccttcttcacccatgcgcaaggaccagcagagtcagttcagt *F ttcccggatgtctctcgcgaggcggaggagagcagccactcatatctggaggtcaacaga *F ggaactgcctatgaccgtcgcctgtcgcaggtcatccaggacaggcataacgtggagcga *F tctgtaaacacaccttgtccaagcagcctgccagaaattaactccgatttatcccttgtt *F ggtggttccgtctatccatctgtcacgcaggaggtcgctgcagtttgtggcgagtgcaac *F gagacggataggaacctctgcagtgtgggtggacttcatatgcccaccagccgatccatg *F caccagctggcaaagaagcgccgcaatcgtatggcaagctacagtggaaatggttcctgt *F gcggacagcagaagttcggcggcaaagaaggcaagttggagtactgaggcagagaaccca *F atgcgacgaaccaaatcctgctcggccctttccggaatgcggcagcagcatctggaggag *F aatgatgacgaggccgattgttcgagccaaagacaaagaggggagaatggaaatacgcaa *F aagactggcagccgcctgcagaggagcggccggaacctggccatttcggcgcccaaggat *F acggctagaagctgcacccatgcctccacccagacggtggaaggactggacagtgctcca *F gcgcagtacgagaattggcttattgaactcctgctggagtgcaaggagcaaagaatcgac *F tatgaaaggaaccttctgtacccgcaagatattctagacgaatacattaagcatgcgatc *F aaggccaatgagtcctttgacgccgagcagggtcaactgatgtgcctacagctggaatac *F gaaagctaccgtcgatccattcacgcagagcgcaatcgacgactcatggggcgaagcagg *F gacaagcgcagcctggaaatggagcgggatcggttaagggagcagcttaagaacttcgat *F gcgaagaacaaggatctggcaaacaaaatggatcaggccattcggttggccaacgagcgc *F cagaacatccaccaggaggagctgggcgaaatgagggctaagtaccagcacgaactggag *F gaaaagaagtgcctgcggcaggcaaacgatgacctgcagacgcgactcaccagcgagttg *F gcgcgccacaaggagatgaactatgaactggagtctctgcgaggtcaggtcttcagtttg *F ggaaccgagcttcaacacacccagcagcaggcggacattgggctgcagtgcaagcaggag *F ctggcacgactggaggccgagtttattatcatgggtgaggtgcaagtgcgttgccgcgac *F cgtctggctgagatcgataacttcagggcccgcgacgaggaactgcagatgctacaagag *F agcagcaacctggaactgaaggatctgaggcacagcctggacgagaagacatcacagctg *F gaaagcatgaagcacaagatcagcgacctgcaggcccagctagccaacagcgagaaggcc *F atgacggagcaaaagcgacttctaagcaccgtcaaggatgagtacgaagaaaagtttaag *F tccgtgaacaagaagtatgacgtgcaaaagaagataattatgcagatggaggagaagctg *F atgatgatgatgcagcagccgcaaggaacaacaggtcataacacctgttccccggacacg *F gacagaactggtgagtgcaagagctgcttcgcagctaaagaatacttactattgcattct *F cccacagacatagcttcatccattgaacgcaactcaccgctatccacgtcgctggcctcg *F agcgagagcttatccgccagcctacgctccacggagctgaagaacctgcaccagctagtg *F gacacgcccactattccggatgtgctgaacagcatggccggtggcgctcagttcgaggac *F gaagtgcgtccgccggccgtggatctggcctcctcggcaagcaccgccagtgccatcaac *F atcgtgccgcacgccttggacttgccgtcgacctccggcggcatcggtcacacgctcacc *F cacccacatccgcatccgcacctgcacctgcagcaacagcaacaggatcaactgcagtag *F >AE003746|GENSCAN_predicted_peptide_21|1398_aa *F MEEFEQEPFEVGEFIERLTWRTNNELQNSEDFHPVALHDTFIQTIKDLKILQEKQQSKCE *F RLEESLRQEKESHAKKIAKLQERHQTAIDVFGQLDEKINSVAGKIMHLGEQLENVNTPRS *F RSVEAQKLLNFMSEFLAAGPVIVNDIFADAARLSEAADVIQKLYAISQDLPPGNFAESKR *F KIEKKYDEVERRLIEEFATAQKSEDIERMKTLAQILSQFKGYTQCVDAYIEQSQMQPYSG *F KDIFIGIVPLCKHHYEIIQKVFANPQQVMSKFILNIYQLKLHQYAMTKLEDKKDEEKYLR *F TLYELYSRTLKLSTDLQIYMSTIDDDLLQKLTQQIFIKHLAGYAEMETKCLTAKCSTELE *F KFYASKKHQKTATTKGFRRNMEVLIATRANINIAAIEDYGGETFLSEELAINMLQEAKAS *F LKRCRLLSNETELPGNAIKLNDILLRFLMHEHVDYALELGLQAVPLAEGRVFPQLYFFDV *F VQKTNIIVHLLDKLCHTSVIPCVSNTPKYSDYVFKKRILMEQIETKLDQGLDRSISAVIG *F WVKVYLQYEQKKTDYKPETDVDTISSAACLQVVQNLQPVIVQIKKCVDGENLQNVLTEFG *F TRLHRVIYDHLQTMQFNTAGAMCAICDVNEYRKCIRELDSPLVTQLFDILHALCNLLLVK *F PQNLQEVCTGDTLNYLDKSVVRQFIQLRTDFRIIKNTNYLKGSLYQVSYLDIESCTTLPC *F SMARNATIKVTVRFDDNGNGVSFLKHEVRWVFNYIKTQAAITPDPCDGDHGCIESASGGK *F AYWANIFVNETLPVINSSQERKSEQCGELKIKVATSDQRRRSDCVGVRDHGSRGVEPVHP *F KVFPLPLQSPRIGAFQMGSMQVALLALLVLGQLFPSAVANGSSSYSSTSTSASNQLQRQK *F LAHWFRDSNDVKDKILELQCLAKCGSNPTTKAGREQCLNKCIQELLLGPRAGSCPKIGRQ *F SRARLSCLDNCQYDHECPEVQKCCPSSCGPMCVEPLGVRNNTQLPPIPKILYFRRSRGHA *F VDLKIESSLLVYYFHVEVRSHIGRHFAARKLGPWQWQKVEKTMEENIGHSKHTYIFFHMR *F PGRWYEVRVAAVNAYGFRGYSEPSDPFPSTGNPKPPKSPNDSKIIGKQFDGRYMTLKLVW *F CPSKSNLPVEKYKITWSLYVNSAKASMITNSSYVKDTHQFEIKELLPNSSYYIQVQAISY *F LGSRRLKSEQWSMLFNTTLQPLEPITPLQCSGNGNRRRHHHTSSSMSSERATTSEPVALN *F EVSPTITNRTSAAATYEVGFRLNRKFGMIVQILGFQPHKEKVYELCPQETNCEQREFRAI *F RAKVSNTLDDALMGATSHMSEYFVPIGRTTGQGQISGTSLTMGPNSVLDDSRNVFTFTTP *F KCENFRKRFPKLQIKCSD *F >AE003746|GENSCAN_predicted_CDS_21|4197_bp *F atggaggagtttgaacaggaaccctttgaggtgggcgagttcatagagcgtctgacttgg *F cgcaccaacaatgaactccagaacagcgaggactttcatcctgtggccctccacgatacc *F ttcatccagaccattaaggacctaaagatcctgcaggagaagcaacaaagcaagtgcgaa *F cggctagaggagtcactgcgccaggagaaggagtcgcacgccaaaaagattgccaagctc *F caagaacgccaccaaacggccattgatgtgttcggccagctggacgaaaaaatcaattcg *F gtggccggcaagatcatgcacctgggcgaacagttggagaatgtgaacactccacgcagt *F cgttcagtggaggcccagaagttactcaattttatgtccgagtttttagccgctggccct *F gtgattgtcaacgatatttttgcggatgccgcaagattaagtgaggccgcagatgtgata *F caaaagctctacgctatctcgcaggatctgccgcccggaaactttgcggaatccaaaaga *F aaaatcgaaaagaaatacgatgaagtcgagcggcggttgatcgaagagtttgccaccgcc *F cagaagagcgaggacatcgagcgcatgaagacactggcccagatcttgtcccagttcaag *F ggttacactcagtgtgtagacgcatacatcgagcagagccaaatgcaaccgtacagtggc *F aaagacatatttataggcattgtaccgctatgcaagcatcactacgagataatccagaag *F gtgtttgccaatccgcagcaggttatgtccaagttcatacttaacatatatcaactgaag *F cttcaccagtacgccatgactaagttggaggacaaaaaagacgaggagaaatatcttcgc *F accctttatgagttatactcgcgcacactaaaattgtcaacagatcttcaaatctacatg *F tccacgatcgacgacgacttgttacagaagctgacacagcagatttttataaagcatttg *F gccggctacgccgaaatggagaccaaatgcctcacagccaagtgttccacagagctggaa *F aagttctatgccagcaagaaacaccaaaagactgcaactactaagggctttcggagaaac *F atggaggtgctgatagccacgcgggctaacataaatattgctgccatcgaggactatggc *F ggggagacgttcctatccgaagagttggccatcaacatgctgcaggaggccaaggcgtcg *F cttaagcgctgtcgcctgctgtccaacgagaccgaactaccaggcaatgctataaagcta *F aacgatatccttcttcgtttcttgatgcacgagcacgtagattacgcactggagttggga *F ctgcaagcagtgccactggccgagggcagggtctttccccagctctacttctttgatgtg *F gtgcaaaagacgaacatcattgtccatctactggacaagctgtgccacacatctgtcata *F ccctgtgtgagtaatacacccaagtactcggactatgtgttcaaaaaacgtattctgatg *F gagcaaatcgagacaaagctggaccagggtcttgatcgctctattagcgctgttattggc *F tgggtcaaggtatatttgcaatatgaacaaaagaaaacggactacaagccggaaacagat *F gtggatacaatatcttcagcggcctgcttacaagtcgttcagaatctgcagcccgtgatt *F gtgcagattaaaaaatgtgttgatggagagaatttgcagaatgttcttacagaatttgga *F actcggttgcaccgagtaatctacgatcacctgcagaccatgcagttcaacacggctggc *F gccatgtgtgccatctgcgacgtgaacgagtatcgcaagtgcattcgcgagctggatagt *F ccactggtcacgcagctgtttgacatactgcatgcattgtgcaatttactacttgttaag *F ccccaaaaccttcaagaagtttgcacgggtgacactctgaattatctggacaagtcggtg *F gtgcggcaattcattcagctgcgcactgatttcaggatcatcaagaacaccaactatctg *F aagggttctttgtaccaggtcagctacctggacatcgagagctgtacgacactgccctgc *F tccatggcccggaacgcgacaattaaggttactgtgcgcttcgatgataatggcaatggc *F gtcagctttctgaagcacgaagtccgatgggtgtttaactacatcaagacccaggcggcc *F ataactcccgatccctgcgacggagatcacggatgcatagagagcgcaagtggtggaaag *F gcctattgggccaatatctttgtgaacgaaactttgccggtgatcaacagttcccaggag *F cggaaatcggaacagtgcggcgaattaaagattaaagtggcaacaagcgatcaaagaagg *F cgctccgactgcgtgggagtacgagatcacggatcacgaggcgtggagccagtgcatccg *F aaagtgtttccattaccattgcaatcaccgaggatcggagcgtttcagatgggcagcatg *F caagtggcgctgctggcgctgcttgttctcggccagctattcccaagcgccgtggccaat *F ggatcctcctcctatagttccacctccacatccgcatcgaatcagctgcagcgacagaag *F ctggcacactggttccgggatagcaatgatgttaaggataagatcctggagctgcaatgc *F ctggcgaagtgtggcagcaatcccacaaccaaagctggacgggaacagtgcctgaacaag *F tgcatccaggagcttttgctgggacccagagccggcagttgccccaaaattggaaggcaa *F tcgcgtgccagactctcctgcctggacaactgtcagtacgatcatgaatgcccagaggtg *F cagaagtgttgtccctccagttgcggacccatgtgcgtggaacctctcggcgttaggaac *F aacacacagcttccgcccataccgaagattttgtatttccggagatcgcgaggtcatgct *F gtcgatctgaagatcgagtcctcgctactggtctactacttccatgtggaggtaagatcc *F cacataggacggcattttgcagccagaaaactgggtccttggcaatggcagaaggtggag *F aagaccatggaggagaacatcggacacagcaagcatacttacatcttctttcacatgcga *F cctggtcggtggtatgaggttcgagtggcagccgtaaacgcctacgggttccgtggatat *F tccgagccaagcgatccctttccctcgacgggcaacccaaagcccccaaagtctccgaac *F gattcgaagatcatcggcaagcagttcgatggacgctacatgacccttaagctggtgtgg *F tgcccctccaagtccaacctgcctgtcgagaagtacaagatcacctggtcattgtacgta *F aacagtgccaaggcctcgatgattacgaacagctcctacgttaaggatacacaccagttc *F gaaatcaaggaactgctacccaactcctcgtactacatccaagtgcaggccatatcctac *F ctgggttcgcgtcgcctcaagtccgagcagtggtcgatgctgttcaacacgacgctgcaa *F cctctggagccaattacaccgcttcagtgctccgggaatggcaataggcgacggcatcat *F cacactagcagctctatgagctcggaacgggccacaacctcggagccagttgccctcaat *F gaagtttcgcccaccattacaaaccggacatcggccgccgccacgtatgaagtgggtttc *F cggttaaaccggaagttcggcatgattgtgcagattctgggcttccagccacacaaggag *F aaggtctatgaactgtgtccccaggagacgaactgcgagcagcgagagttccgcgcgatt *F cgcgccaaagtaagtaatacccttgatgatgctctgatgggagccacgagccacatgtcg *F gaatacttcgtgccgattggccggaccaccgggcaagggcaaattagtggaacatcgttg *F accatgggtcccaattccgtgttggacgactccagaaatgtctttaccttcaccacgcct *F aaatgtgaaaatttccgcaagagatttcccaagctgcagatcaagtgcagcgactag *F >AE003746|GENSCAN_predicted_peptide_22|464_aa *F MLSLRSVLKHCLSAKKTCSRNISALYITGDKANENYVTLQPYMDFNKTFGERQFLEQSIS *F SRGLDIRLETVLSKYEKYKTHHAQLSKVAEERERVTKRLKELTKSGSSAVQLEELKEHGK *F SLRNELKALKQTLYPIEDDFIHDYLHLPNLLHVQCPVGGEEKLLYRHGIPKSENKTTSHL *F ARQELVHFVDNNRYYLMEQAALFDVNAMQSLARYFVNHGHFIQTANPDFVRCVLLEANAT *F PLSDYHLVQEEHLQNKINTAYLTGGASFESYLGAMTKLCVYPSVLPLRYVCCGRSYNRAE *F ADLYGPIPSLYTATQTNAVQIFVATQTDNEADSQLEHILNLATDFYKALDIPFRISYATA *F ADLTPAESIRAVIEVYAPSLQRYVCVGRISNYGDFVSKRILFSTRREKHYDFLHMVGGPV *F LYTSRLIAALVEHGVRLEDCKLLGSISQKPVHQQDLQQFKDLFT *F >AE003746|GENSCAN_predicted_CDS_22|1395_bp *F atgttgagcctgcgaagtgtattaaaacactgcctttcggcaaagaaaacgtgtagcaga *F aacatctccgcgctgtacataaccggcgataaagcgaacgaaaactatgtgactctgcag *F ccgtacatggacttcaataaaacctttggagagcggcaatttttggagcagagcatctcc *F agccggggattggacattcgtttggaaactgtgctcagcaagtacgagaagtataaaaca *F catcacgctcagctgtccaaggtggcggaggaacgcgaaagggtgaccaagcgcctaaag *F gagctaacgaaatctggtagtagtgcggttcagctggaggagttgaaagagcatgggaaa *F tcgttgcgcaatgagctaaaggctctgaagcagactctctatcccatagaggatgacttt *F attcatgactatctgcacttgcccaaccttctgcatgtccagtgccctgttggaggggag *F gaaaagcttctctatcgtcatgggatacccaaatcagaaaataagactacatcccacttg *F gcacgccaggaacttgtacattttgtagacaacaatcgttactacttgatggagcaggct *F gctctttttgacgtaaatgccatgcaatccctggcccgctatttcgtcaaccacgggcac *F ttcattcaaaccgccaatccggactttgtgcgttgcgttctcctggaagcaaatgcgaca *F cccttatccgactatcatctggttcaggaagagcacctgcaaaacaagatcaataccgcc *F tatttgactggaggtgcgtccttcgagagctaccttggtgccatgaccaagctgtgcgtg *F tatccctcggtgctgccccttcggtatgtctgctgtggtcggagctacaacagagcggaa *F gcggatttgtatgggcccattcctagtctttatacggcgacacaaacgaatgcggtacaa *F atctttgtggcaacgcaaacggacaacgaagcggactcccaactagagcatattctcaat *F ctggcaactgatttctacaaagcactggacataccattcaggatttcttatgcaacagca *F gcagatctaactccggcggagagtatccgggcagtcatcgaagtttatgccccctcactg *F cagcgctatgtgtgtgtgggacgtatcagcaactacggagactttgtctctaagcgaatc *F ctttttagcacgcggcgggagaagcactatgatttcttgcacatggtgggcggaccagtg *F ctctacacctcgcgactgatagcagctcttgttgagcatggcgtgcgcttggaagactgc *F aaacttttgggctctattagtcagaaacccgttcatcagcaggatctacagcagtttaag *F gaccttttcacgtaa *F >AE003746|GENSCAN_predicted_peptide_23|356_aa *F MPEFVRVAINESLWEFPDIYEFVRFLGGGSFGQVAKVRLRGTENYFAMKRLMRPFEREED *F AKGTYREIRLLKHMNHRNVISLLNVFHPPAHNMMEFQQVYLVTHLMDADLHRYSRSKRMS *F DQEIRIILYQILRGLKYIHSAGVVHRDLKPCNIAVNGNSEVRILDFGLSRMCADKMTDHV *F GTMWYLAPEIIFLRGQYTKAIDVWSVGCILAELITDRVLFRGENYVSQIRCLINIMGTPT *F REFITGISMERSRNYLEGYPLRQRCDFHHLFMGYDVQAIDLMEKMLEMVPEKRITAAEAM *F LHPYLRDLIEPHHHAEDTAPVYDQNFENMVLPVKCWKELVSHEIRNFRPDQLDLHF *F >AE003746|GENSCAN_predicted_CDS_23|1071_bp *F atgccggagttcgtgagagtggcaattaacgaaagcctttgggagttcccggatatatac *F gagttcgttcgttttctgggtggcggttcctttggccaggtggctaaggtgagactacga *F ggcactgaaaattacttcgctatgaaaaggcttatgcgcccattcgagagggaggaggat *F gctaagggtacctatcgcgagatccgtctgctgaagcacatgaatcatcgaaatgttatc *F agcctgctgaacgtcttccatccaccagcgcacaacatgatggaatttcagcaggtttat *F ttggtgactcacctgatggacgcggatttgcacaggtactcgcgttcgaaaaggatgagc *F gatcaagagattaggataatcctttaccaaatactgcggggactgaagtacatacatagt *F gccggggttgttcatcgagacttaaagccctgcaatatcgcagttaatggaaatagcgag *F gtgcgcatacttgacttcggtttatcccgtatgtgcgcagacaaaatgacggaccatgtt *F ggaactatgtggtatctagctccggaaattatctttttaaggggtcaatacacaaaggca *F attgacgtgtggtcggttggttgcattctggcggaacttatcacggatcgtgtcctgttt *F cgcggtgaaaactatgtaagccaaatacgatgtctgattaacataatgggtactccgacg *F agggagtttatcaccgggataagcatggaacgttcgcgtaattacctggaggggtacccg *F ttacgacaaaggtgcgattttcatcacctgtttatgggttacgatgtccaggccatcgat *F ttgatggaaaaaatgctcgaaatggtacccgaaaaacgtatcacagctgcggaagcaatg *F ctccatccataccttcgggatcttattgagccacaccatcatgccgaagacaccgcacca *F gtctatgatcagaacttcgaaaacatggtactgcctgtaaaatgctggaaagaacttgtt *F tcccacgagattcggaacttcagacctgaccaacttgatttgcatttttaa *F >AE003746|GENSCAN_predicted_peptide_24|366_aa *F MSVSITKKFYKLDINRTEWEIPDIYQDLQPVGSGAYGQVSKAVVRGTNMHVAIKKLARPF *F QSAVHAKRTYRELRLLKHMDHENVIGLLDIFHPHPANGSLENFQQVYLVTHLMDADLNNI *F IRMQHLSDDHVQFLVYQILRGLKYIHSAGVIHRDLKPSNIAVNEDCELRILDFGLARPTE *F NEMTGYVATRWYRAPEIMLNWMHYDQTVDIWSVGCIMAELITRRTLFPGTDHIHQLNLIM *F EMLGTPPAEFLKKISSESARSYIQSLPPMKGRSFKNVFKNANPLAIDLLEKMLELDAEKR *F ITAEEALSHPYLEKYAEPSVEQTSPPYDHSFEDMDLPVDKWKELIYKEVTNFKPPPSYAQ *F VLKDVK *F >AE003746|GENSCAN_predicted_CDS_24|1101_bp *F atgtcagtgtccattacaaaaaagttttacaagttggatataaatcgaacggaatgggag *F atcccggatatataccaggatctgcagcccgtgggatcgggagcttacggacaggtgtca *F aaggcagttgttcgtggcaccaatatgcatgtggccattaaaaagcttgccaggcctttt *F caatcagctgtccatgcaaagaggacgtaccgggagcttcgacttttaaagcatatggat *F catgagaacgtaatcggtctgctggacatattccatccacatcccgctaatggatcgctg *F gagaacttccaacaggtgtacttggttacccacttgatggacgcagatctgaacaacatc *F atacggatgcagcacttgtccgacgaccacgtccagtttttagtctaccagatactccgt *F ggcttgaagtatatccacagcgccggagtgatccaccgtgatcttaagccctcaaacatt *F gccgtcaacgaggattgcgagctgcgcattctagacttcgggctggcccgcccaacggag *F aacgagatgacaggctatgtggccacgcgttggtaccgggcacctgaaataatgctcaat *F tggatgcactacgaccaaacagtggacatctggtcggtgggctgcatcatggccgaacta *F attaccagacgaaccctcttcccaggcaccgaccatattcaccagctaaacctgattatg *F gagatgttgggcacgccacccgccgaatttttgaagaagatctcatcggaaagtgcacgt *F tcctacatccagtcacttccgcctatgaagggacgaagttttaaaaatgtttttaagaac *F gccaatccgctggccattgatttgctggaaaagatgttggagctagatgccgaaaagcgg *F atcacagccgaggaggctctttcccatccatatctggagaagtatgcggagcccagcgtc *F gagcagacctcaccaccatacgatcacagcttcgaggatatggatttgcccgtagacaaa *F tggaaggaattgatctacaaggaggtcaccaactttaagcccccaccatcgtatgctcag *F gttctaaaggatgtaaagtga *F >AE003746|GENSCAN_predicted_peptide_25|544_aa *F MTSKLLPGNIVYGGPVTERQAQDSRSLGQYILDKYKSFGDRTVLVDAVNGVEYSASFMHK *F SIVRLAYILQKLGVKQNDVVGLSSENSVNFALAMFAGLAVGATVAPLNVTYSDREVDHAI *F NLSKPKIIFASKITIDRVAKVASKNKFVKGIIALSGTSKKFKNIYDLKELMEDEKFKTQP *F DFTSPAANKDEDVSLIVCSSGTTGLPKGVQLTQMNLLATLDSQIQPTVIPMEEVTLLTVI *F PWFHAFGCLTLITTACVGARLVYLPKFEEKLFLSAIEKYRVMMAFMVPPLMVFLAKHPIV *F DKYDLSSLMVLLCGAAPLSRETEDQIKERIGVPFIRQGYGLSESTLSVLVQNDEFCKPGS *F VGVLKVGIYAKVIDPDTGKLLGANERGELCFKGDGIMKGYIGDTKSTQTAIKDGWLHTGD *F IGYYDDDFEFFIVDRIKELIKYKGYQVPPAEIEALLLTNDKIKDAAVIGKPDEEAGELPL *F AFVVKQANVQLTENEVIQFVNDNASPAKRLRGGVIFVDEIPKNPSGKILRRILREMLKKQ *F KSKL *F >AE003746|GENSCAN_predicted_CDS_25|1635_bp *F atgacttcaaagctactgcccggaaacattgtgtacggaggtcctgtgactgaacgacag *F gcccaggatagcagatccttgggtcagtacatcctcgacaagtacaagagctttggcgac *F cggacggtgctggtggatgccgtcaatggagtggagtactctgccagtttcatgcacaag *F tccattgtacggctggcatacatccttcaaaaactgggagtcaaacagaatgacgtcgtt *F ggtttgtctagcgaaaacagcgtcaacttcgccctggccatgttcgctggtctagcagtt *F ggggctacggttgctccccttaacgtaacatattccgatcgtgaggtggaccacgccatt *F aacttgtccaagccaaagatcatattcgcctctaagattaccattgatcgtgttgccaaa *F gtggccagcaagaataagttcgtcaagggcatcattgcgctcagtggaacttccaagaaa *F tttaagaacatctatgatcttaaggagctgatggaggacgagaagttcaagacacagcct *F gacttcacgagccctgcggccaataaggacgaggacgtgtctcttattgtgtgctcttct *F ggaaccaccggacttcctaaaggagtgcagctgacccaaatgaacctgctggccactctc *F gactcacaaatccaacccactgtcattccaatggaggaggtcactctactcactgtcatt *F ccctggttccacgccttcggctgtctgacgcttatcaccaccgcctgcgttggcgcacga *F ttggtatacctgcccaagttcgaggaaaagctcttcctttctgccattgaaaagtatcgc *F gtgatgatggccttcatggtgccaccactgatggtctttttggctaaacaccccatcgtg *F gataagtacgatttgtcctctttgatggtcctgctgtgtggagcagctccactcagtcgc *F gaaactgaggatcagatcaaggagcgtattggagtgccattcatccgacagggatacggc *F ctcagcgaatcaacgctgagtgttctggtgcagaacgatgagttctgcaagccaggcagt *F gtgggcgttcttaaggtgggaatctatgccaaggtgatcgatcccgacaccggcaagcta *F ttgggggccaacgagcgcggcgagctttgttttaaaggcgacggcatcatgaagggctac *F atcggagatacgaagtccacgcagaccgccatcaaggacggttggttgcatactggcgat *F attggctactatgatgatgattttgagttcttcatcgtggaccgcatcaaggagctgatc *F aaatacaagggataccaggtgccgccggcagagattgaggctctgctgctcaccaacgac *F aagattaaggatgcggcggtcattggaaagccagacgaggaggctggcgagctgccgctg *F gcatttgtggtcaaacaggctaatgttcaactgaccgagaacgaagtcattcagtttgtc *F aacgacaacgcctcgcccgccaagcgtctaaggggtggcgtgatctttgttgacgaaatt *F ccaaagaaccccagtggcaagattctgcgtcgcattctgcgggaaatgcttaagaagcaa *F aaatccaagttgtaa *F >AE003746|GENSCAN_predicted_peptide_26|1082_aa *F MEQEIGTWDSVLLENLSEDSFINNIHQRYKRDHIYTYIGTSVVALNPYHHISEHSLDNVR *F NYGDKGIFQLPPHIYGLTNLAYQSLKDQSEDQCVLLTGESGAGKTETFKMIVNFLTHIQD *F RSHCPPTPNVLRKQSSTSSASGLVMHAHRRASSSCSGTANFIICKNRAENPSGSVSRRQS *F PSPGPSQRSRTRAESIERQSRRHMREKIVDFDFSHHKSSENISGLPESHAHHMHPTKSCF *F KHQQTQVSACTAMPAAAKGSPKYAVPTVYGGCRQCGHSKCVRAQSLEKEERDDLRGSNCR *F LSTIATATTNPAHPHRGSCSNLMRQHSTESQPERERDRSSLMGSTQRISLYDAHKLSKVL *F GDLPPPPPSSVSPTPSASSSLHRRHKSPTQRMRECVTCADVFLEAMGNACTLKNNNSSRY *F TRITDPIIGERNFHIFYQLLLGADLQLLKSLKLYRNVEKYELLRNTTAMEEDRMNFHYTK *F NFVFHVLRSEQELYIREGLEWSRIDYFDNESICELIDKPSYGILSLINEPHLNSNDALLL *F RVQQCCAGHPNFMTTGSNSMCFQIRHYASVVNYSIHRFLEKNSDMLPKYISAAFYQSKLS *F LVQSLFPEGNPRRQVTKKPSTLSSNIRTQLQTLLAIVKHRRSHYVFCIKPNEGKQPHQFD *F MALVQHQVRYMSLMPLVHLCRTGHCYHLLHVKFFHRYKLLNSLTWPHFHGGSQVEGIALI *F IRNLPLPSAEFTIGTKNVFVRSPRTVYELEQFRRLRISELAVLIQTMFRMYHARKRFQRM *F RHSQMIISSAWRTWRAREEYRSLKYKRQVRWAIDIIGRYYRQWKIRQFLLTIPLRLPPNT *F LSPLSTEWPVAPAFLADASRHLRSIYHRWKCYIYRNSFDQTARNRMREKVTASIIFKDRK *F ASYGRSVGHPFVGDYVRLRHNQQWKKICAETNDQYVVFADIINKIARSSGKFVPILLVLS *F TSSLLLLDQRTLQIKYRVPASEIYRMSLSPYLDDIAVFHSEFGRKKGDFVFQTGHVIEIV *F TKMFLVIQNATGKPPEIHISTEFEANFGQQTVIFSFKYGGMSDLAQGPPKVTRKANRMEI *F IV *F >AE003746|GENSCAN_predicted_CDS_26|3249_bp *F atggagcaggaaatcggcacctgggactcggtactgttggagaacctgtccgaggatagt *F ttcataaacaacatccaccagcgctataagcgcgatcacatatatacctacattggaaca *F tctgttgtggctctgaatccatatcatcacatatccgagcactctctggacaatgtccgc *F aactatggcgataagggcattttccagctgccgccccacatatatggtctcacaaatctg *F gcttatcaatcgctcaaagatcagagcgaggatcagtgtgttctgctcaccggtgagagc *F ggagcgggcaaaacggagacttttaaaatgatcgtgaactttctgacccacatacaagat *F cgctcccactgccccccaacaccgaatgttttgcgcaagcaatcctcaactagctcggcc *F agcggattggtgatgcacgcccacaggcgagcctccagcagctgctccggcactgccaat *F tttattatatgcaaaaaccgggcggaaaatccgtcaggcagtgtttcacggcgacaaagt *F ccatcgccaggaccatcgcagcgatcgcggacgcgggccgagagcatcgagcgccaaagc *F aggcgccacatgcgggagaaaattgtcgactttgatttctcacaccacaagagtagcgaa *F aacatcagcggccttcctgaatcgcacgcccaccacatgcatccgaccaagtcgtgcttc *F aagcaccagcagacccaagtcagcgcctgcactgcaatgcccgcagcagccaagggatcg *F cccaaatacgcggtacccaccgtgtacggcggttgccgccagtgcggacacagcaagtgt *F gtccgtgctcagagcctggaaaaggaggagcgggatgatctacgaggcagcaactgccgc *F ctgtctaccatagccactgccaccaccaatccggcacatccgcatcgcggtagttgctcc *F aacctgatgcgccagcactcgacagagagtcagccggagcgggagcgggatcgaagctcc *F cttatggggtccacgcaacgtatatcgctgtacgatgcacacaagctgagtaaggttctg *F ggcgatctgccgccgcctccgcctagttctgtttcgcccacgccatcggccagctcttcg *F ctgcacaggcgtcataaatcgcccacgcaacgaatgcgagagtgcgtcacctgtgcggat *F gtgttcctggaggccatgggcaatgcctgcaccctgaaaaataataactctagtcgatat *F acccgtataacggaccctatcattggagagcgaaattttcacatcttttaccaattacta *F ttaggagctgatctccagttgctaaaatcgctcaagctgtatcgaaatgtggaaaagtac *F gagctgctccgcaacacaactgccatggaggaggaccgcatgaattttcattatacgaag *F aattttgtgtttcatgtgctgcgttcggagcaagagctctatattcgcgagggattggaa *F tggtctcgcattgactatttcgacaacgagtctatttgcgagttaatagacaaacccagc *F tatggtatattgagcttgattaatgaaccccatttaaatagcaacgacgctttgcttttg *F cgagttcagcaatgttgtgcggggcatcccaactttatgaccaccggcagcaattccatg *F tgctttcagattcgtcattatgcaagtgtagtgaactactcaatacatcggtttctcgaa *F aagaactccgacatgctgccgaagtacataagcgctgccttttatcagagcaaactttct *F ttggtgcaaagcctattccccgaggggaatccccgtcgacaggttaccaaaaagcccagc *F acgttgagttcgaatatccgcacccaattgcagacgctgctggccatcgttaagcatcgc *F cgctcccactatgtgttctgtattaagcccaacgagggcaagcagccgcaccagttcgat *F atggctctagtgcaacatcaggtgcgctacatgtcgctcatgccgctggtccacctgtgt *F cgcactggccattgctaccacctgttgcacgttaagttttttcatcgctataagttgctc *F aacagcctgacgtggccccactttcatggcggcagtcaggtagagggtatcgccctcata *F atccgtaacctaccgctgccctcagcggagttcacgatcggcaccaaaaatgtgttcgtg *F cgtagtccccgcaccgtatatgagttggaacagtttcgccgcctgcgtattagcgagctg *F gccgtgcttattcaaaccatgttccgaatgtatcacgcaaggaagcgctttcagcgcatg *F cgacacagccagatgatcatatcgagtgcctggcgcacgtggcgggcccgcgaggagtat *F cggtccttgaagtacaaacgacaggtgagatgggccatcgatattataggccgctactac *F cgccagtggaagatcagacagttccttctgacaattcccttgcgactgccaccgaacacg *F ctaagcccgctctccaccgaatggccagtggctcccgcatttctggcagatgcctctcgt *F catcttaggtccatataccatcgttggaagtgctacatctaccgaaactcctttgatcaa *F acggcgcgtaatcgaatgcgggagaaggtcacagccagcattatcttcaaggatcgaaaa *F gcttcatatggacgaagtgtgggtcatccttttgtgggggactacgtgcgactgcgacac *F aaccagcagtggaaaaagatctgcgccgagaccaacgatcagtatgttgtattcgcagac *F ataatcaacaagatagcgcgctccagtggcaagtttgtgcccattttgctggtgctatcc *F acgtcatcgcttttgctgttggaccaacgaacgctgcaaattaagtacagagtgcctgca *F tcggagatttaccgaatgtctctgagcccctacctagatgacattgctgtgtttcactct *F gaatttggacggaagaagggtgatttcgtttttcaaacgggtcatgtgattgaaattgtt *F accaaaatgtttctggtcatacaaaatgccacaggcaaacccccggagatacacataagc *F actgaatttgaagcgaacttcggccagcagactgtcatcttttcgttcaaatacggcggc *F atgtcggacttagcacaaggcccacccaaggtcacacgcaaggcgaaccgcatggagata *F attgtgtga *F >AE003746|GENSCAN_predicted_peptide_27|306_aa *F MNTDERNFRSIYYEKCQINSVEEQKSLNKLLQDDIRNLSKLKQFCMNYTVPNNNRSYLWA *F LVMGILPLHKASTAYVRDQRREMYEDLRRAVTVLRFTDHKQKEQPFMWLIDHKKKAQVMH *F TMWLIESNRLWHGNTSASLQADDMHFIEIVRTLLQIFDDNVETYWIAKGFYKYTRELKKE *F CVKLKEQTQNILKREDLSLLNHLELLGLFDGNSTLLDNWYITCFAGIICTTHLVKIWDKV *F CGGSRKIVVFLFVELVKDIRSSILKQTSLADVKRLIETVKDLDGVIIVNKAIKSLQNNSS *F EVEYTH *F >AE003746|GENSCAN_predicted_CDS_27|921_bp *F atgaatactgacgagcgaaacttcaggtccatctactatgaaaaatgccaaataaacagc *F gtcgaggaacaaaagtcgctgaacaaactactacaggatgacatccgcaacctgagcaag *F ctgaaacaattctgcatgaactacacagtgccgaacaacaataggagctatctgtgggcc *F ctggtcatgggtattctcccgctccacaaagcgtccacggcgtacgtacgcgaccaaaga *F cgcgaaatgtacgaggatctgcggcgagcggtaaccgtgctccggtttaccgaccataag *F cagaaggagcagcccttcatgtggctgatagaccataagaagaaagcacaggtcatgcac *F accatgtggctgatagagtccaatcgactgtggcatggcaatactagtgccagtctccag *F gcggacgacatgcacttcatagagattgtgcgcacgttgctgcagatattcgacgacaac *F gtggaaacctactggatagcaaagggattctacaagtacacccgcgaactgaagaaggag *F tgcgtcaagctgaaggagcaaacgcagaatatactgaagcgcgaagatttgtccttgctt *F aatcacctggagcttctgggcctgtttgatggcaactcaacgctactggataactggtat *F ataacctgctttgctggaataatctgtacaactcatttggtcaagatatgggacaaagtc *F tgtggagggtcccggaagattgttgttttcctgtttgtagaactagttaaggacataagg *F tcctcgatactaaagcaaacatctttagcagacgtcaaaagacttatagaaacggtaaag *F gatcttgacggtgttattatcgtaaacaaggccatcaaatcactacaaaacaacagcagc *F gaagtcgagtacacgcactaa *F >AE003746|GENSCAN_predicted_peptide_28|161_aa *F MNFLKKVATEVQQLSRSGFHTSSVCCRVQSGRYRITTKRNRPLTYEMANPPHFIGHRKSW *F NSWNTSTMKDALRPSQTAIEDVFIRKFVTGTWHALVCSEVIIKRQHNTIRIAALIRQAIT *F PRKMYFLIGYTEELLSYWMQCPVTLELQTVGDKKDVVFKYI *F >AE003746|GENSCAN_predicted_CDS_28|486_bp *F atgaacttcttgaaaaaggtggcgaccgaggtgcagcagctcagccggtcaggattccac *F accagctccgtgtgctgtcgcgtgcaatccgggcgataccgcataaccacaaagcgaaac *F aggcctcttacctacgagatggccaatccgccgcattttattggccaccgcaaatcgtgg *F aactcatggaacacgtcaacaatgaaggatgccctgcgtccgtcccagaccgccatagag *F gatgtgttcatccgcaagtttgtcaccggcacatggcatgccctcgtctgctccgaggtc *F attatcaagcgacagcacaacaccattaggatcgcggcccttattcggcaggcgattaca *F ccgcgaaaaatgtacttccttattggttacacggaggagctgctctcctattggatgcag *F tgtccagtgaccttggaactgcaaacggtgggcgataaaaaggacgtggtcttcaaatac *F atttag *F >AE003746|GENSCAN_predicted_peptide_29|1067_aa *F MTLNESDATRLELTRNFLELSRNPETCTALRSSDCIQLLVQILHANDEGLSTAKKYASQA *F LHNIVHNNPEEKERQREVKMLRLLDQILDYCNFLHTQLQSGGEAIADDEDRHPLAAMKLL *F MKASFDEEHRQTMCELGALKAIPNLVHLDHAVHGPAAGREQCNALRSYGLMALTNLTFGD *F ENVHNKSYLCGQRQFMEVVIAQLNTAPDELLQVLAGVLRNLSWRADKHMKTIFNELGTVT *F SLARAAMQNKNENTLKAILSALWNLSAHCSTNKAEFCAVDGALAFLVGMLSYEGPSKTLK *F IIENAGGILRNVSSHIAVCEPYRQILRRYNCLAILLQQLKSESLTVVSNSCGTLWNLSAR *F CPEDQQYLIDHNAIPLLRALISSKNSMIAEGSASALKNLVNFRATQELMPNGDGGSLPLD *F KEAGHGGTLPRRFSSLRLSSNPTGSLKKVRPSTVSTTGFLNRKCESRESIYSGKSDSTKY *F STKSEGAKNPFEIVTPTEEQPIDYSMKYMEHKPNSSKTFEIDLDQPTDFSARYKERRSAQ *F TAQPELKSETNEIRSKELQLTKSSSATELRNSPGLVAVSAAKQKIATETETETAERPINY *F CEEGTPGSFSRFDSLNSLTEKPEKCMPPKTPTKTAVLPVHVDGNTPQNIDSALETPLMFS *F RRSSMDSLVGDDETVACEDNGSVISEYSRMQSGVISPSELPDSPTQSMPQSPRRDRKVST *F QNNLDTPEQKPSTVFEDKLNRFHVEHTPAAFSCATSLSNLSMMDDSNANAIRGQRGNDIN *F GNGDAPRSYCTEDTTAVLSKAPSNSDLSILSIPNDLNANEAQPVPAPRADVTGMDTRMPA *F EDAISKMRCGGNALPSYLPVSDEMSKYYVEDSPCTFSVISGLSHLTVGSAKAGPVLKLPM *F RTAEEAQAPKLPPRRSAVQGDAEPRLPPKKSDSLSSLSMDSDDDCNLLSQAIAAGSCRPQ *F PSGASTSSSLANASTSTLCRENGQSKKQVEHGDKPNYSSDDSLDDDDDDARSKSLFEQCI *F LSGMHKSNDALESEGEPPGQRQEISARDRFVSNQVRQIESMLAGRQH *F >AE003746|GENSCAN_predicted_CDS_29|3204_bp *F atgacgctgaacgagagcgacgctactcgcctggagctgacgcgcaacttcctggaactg *F tctcgaaatccggaaacatgcaccgccctgcgcagctcggactgtatccagcttctggtg *F cagattctgcacgccaacgacgaaggcctctccacggcgaaaaagtacgccagccaggcg *F ctgcacaacatcgtccacaataatccggaggagaaggagcgccagcgggaggtgaagatg *F ctgcgcctgctggaccagatcctcgactactgtaactttctgcacacccagttgcagagc *F ggcggtgaggctatcgcagatgatgaagatcgtcatccgctggcggctatgaagctcctg *F atgaaagccagtttcgacgaggagcaccgccagactatgtgcgaactgggagccctcaag *F gcgattcccaatttggtccaccttgatcatgcggtccatggaccggctgccggtagggaa *F cagtgcaacgccctcaggagctacggcctcatggccctcacgaatctcaccttcggagac *F gagaacgtccataacaaatcgtatctgtgcggtcagcgacagttcatggaagtggtcatt *F gctcaattgaacacggctccggatgaactgctacaggttcttgctggtgtgcttcgcaat *F ctttcgtggcgcgcggacaaacacatgaagactatctttaacgagctgggtactgtgacc *F tccttggctcgagcagccatgcaaaacaagaacgagaacactctcaaggccatactttca *F gccctttggaatctctcagcgcactgcagcaccaacaaggcggagttttgtgcagtagac *F ggagcactggcatttttggtcggaatgcttagctacgagggtccgagtaaaactcttaag *F atcatcgaaaatgcaggcggcattcttcgaaatgtatcgagccatattgcggtgtgtgag *F ccgtaccgacaaatcctaagacggtacaattgccttgccattctgttgcaacagttgaaa *F tcggagagcctaaccgtggtaagcaactcctgcggaactctgtggaatctctcggcgcgc *F tgtcccgaggaccagcaatacctcattgaccacaatgccatcccgctcttgcgggcccta *F atcagctccaagaactccatgattgcggagggcagtgcctcggctttgaaaaacctagtt *F aatttcagggccactcaggagcttatgcccaatggagatggtgggtcactgccactagac *F aaggaagctggccatggaggtacgctgccacggagattcagctcactgcgcctaagctca *F aatcccacgggatcgcttaagaaagtgcgaccctcaacagtcagcacaactggctttctg *F aacagaaaatgtgagagccgagagtccatttactcgggcaaatccgattccactaaatac *F tcaaccaagtcggagggagcgaagaatcccttcgaaattgtgacacccactgaagagcag *F cccattgactactccatgaagtacatggagcacaaacccaatagcagtaagacctttgag *F atcgacttggatcagccaacggatttcagtgctagatataaggagagacgatccgctcag *F acggcacagccggagctgaagtcggagaccaatgagattagaagtaaggagttgcaactg *F acaaagtcctcctcggccacggagctgcgcaatagtcctggcctggtggcggtttcagca *F gctaagcagaaaattgccaccgaaacggagacggaaacggcagagcgaccaataaactac *F tgtgaagaagggactcccggcagcttcagtcgcttcgactcgctcaacagcctcacagag *F aaaccggagaaatgtatgccgccaaaaactccaacgaaaactgcggttctcccggtgcac *F gttgacggaaatacgcctcaaaacatcgattccgcactagaaactccattaatgttttcg *F cgtcgcagctctatggactcattggttggcgacgacgaaacggttgcctgcgaggacaat *F ggatccgtgatcagtgaatacagccggatgcagagtggtgttatttccccctcagagctg *F cccgattcacccacccagagtatgcctcaatctccgagaagggacagaaaagtgtccact *F caaaataacttggacacacctgaacagaagcccagtactgtgtttgaggacaagttgaac *F agattccacgtggagcacacgcctgctgccttctcctgcgccaccagtctaagtaatctg *F agcatgatggacgactctaatgcaaacgctattcgaggacagcgtggaaatgacatcaac *F ggcaatggggatgctcctcgcagctattgcacagaggacactactgccgtgctttccaaa *F gcgccaagcaatagtgatctgtccattttgtccatcccgaatgatctaaatgcgaatgaa *F gcgcagccagtgcctgctccgcgagctgatgttaccggaatggacactcggatgccggca *F gaagatgcaatctctaagatgcgctgcggtggcaatgcattgcccagttatctgccagtg *F tcggatgaaatgagcaagtactatgtcgaggacagtccctgcacgttttcggtcatctcg *F ggactatcccatctcactgttggctctgctaaggctgggcctgttctgaagctgccaatg *F aggactgcagaagaggctcaggcacccaaacttcctcccagacgtagtgccgttcaagga *F gatgcggagccacgcttaccgccgaagaaaagcgactcactgagctcattgtccatggac *F tcggatgacgactgtaatcttctaagtcaggccattgctgcgggaagttgtcgacctcag *F cccagcggtgccagtaccagctccagcctggcgaacgctagtaccagcactctatgcagg *F gaaaatgggcagtcaaagaagcaggtggagcatggcgataagccgaactacagctcagat *F gactcgctagacgacgacgacgatgatgcacggtccaagtcgctatttgagcagtgcatt *F ctgagcggcatgcataagtccaacgacgccttggagtcggagggtgagccgccggggcag *F cgccaggagatcagtgcccgggatcgatttgtcagtaaccaggtgcgccagattgagtcc *F atgttggctgggcgtcagcactag *F >AE003746|GENSCAN_predicted_peptide_30|329_aa *F MTKPTREHCSLALLALLVAPFVVLGEDMPREPDYMKREHSLVRPFQGVGVILPHWDFLGN *F TMVTSNYIRLTPDLQSKSGALWNYSPVMTRNWEVHVGFKVHGKGTELFGDGFAIWYTKER *F MQTGPVFGSKDHFSGLAIILDTYSNHNGPHNHQHPYLSAMVNNGSWSYDHDRDGTHTQLA *F GCEVRFRNVEYETLVSIRYENDILSVSTDLENRNEWKNCFVVANVELPTGYHFGMSATTG *F DLSDNHDIHSFKFYDLDLNVNHDEIIRRSNIIPNAKTFEPPREHKEDPKPGMSNAKIFFI *F LLFVVVVAAAVAIFAISYFKDRNARKRFY *F >AE003746|GENSCAN_predicted_CDS_30|990_bp *F atgaccaaaccgacgcgcgaacactgttccctggccctgctggcgcttctcgtcgccccg *F ttcgtggtgctcggtgaggatatgcccagggagccggactacatgaagcgggagcacagt *F ttggtgcgtccattccaaggcgtaggcgtgatcctgccacactgggacttcctgggtaac *F acgatggtgaccagcaactatataagactgacgccggacttacagtccaagagcggtgca *F ctttggaactactcgcccgtgatgactcgcaattgggaagtgcacgttggctttaaggtg *F cacggcaagggaactgaactgttcggcgacggatttgccatttggtacacaaaggagcgc *F atgcaaaccgggccggtctttggcagcaaggatcacttctccggactggccatcattctg *F gacacctatagcaatcacaatggtccacacaaccaccaacatccatatctcagcgccatg *F gtgaacaatggcagctggagctacgaccacgatcgcgatggaacgcacactcagctggcc *F ggctgcgaagttcgtttccgcaatgtggagtacgagacgctggttagcattcgatacgaa *F aacgacattctgtcggtttccacagatctggagaaccgcaacgaatggaagaactgcttt *F gtagtggccaacgttgagctacccacgggctaccacttcggcatgtctgcgacgacgggt *F gatctgtccgacaatcacgatattcacagtttcaagttctatgacctggacttgaacgta *F aatcacgatgagattatccggcgctccaatatcataccgaatgccaagacattcgagccc *F ccgcgcgagcacaaagaagatcccaagccgggaatgtccaacgccaagatcttcttcatc *F cttctcttcgtggttgtcgtggcggctgcagtggccatcttcgccatctcctacttcaag *F gatcgcaacgcgcgaaaacgtttctactga *F >AE003746|GENSCAN_predicted_peptide_31|130_aa *F MPKYGDDDKSTPCIITRNLVLAFAFALQFGFVLWLVTIATLCLLVAFAAGQQYFLGQFPS *F RTRFGFDPVALAGPSSATQVRDPRQNRGPVVFPPSPPDAVDESSGVVVGASGYGFVPPQQ *F SNANLFKRTV *F >AE003746|GENSCAN_predicted_CDS_31|393_bp *F atgcccaagtacggggacgacgacaagtccactccgtgcataattactcgcaatttggtt *F ttggcttttgcttttgctttacaatttggttttgtgctctggttagtcaccatcgccacc *F ctctgcctccttgtggccttcgccgcgggccagcaatactttctgggccagttcccaagc *F cggactcgttttggattcgatcccgtggcgctagcgggaccctcatcggccacacaggtt *F cgggatcctcgacagaacaggggacccgtcgtcttccccccatccccaccagacgcagtg *F gacgagtccagcggcgtggttgtgggtgcctccggatacggctttgtgccgccccagcaa *F agtaatgcgaacctctttaagcgcactgtctga *F >AE003746|GENSCAN_predicted_peptide_32|52_aa *F MLLSVRTAAAAVAVAAAGDPSTYFSTTFQTPDTAYATYNFFGSPYTTRFRYF *F >AE003746|GENSCAN_predicted_CDS_32|159_bp *F atgctcttgtctgttcgaacagcagcggcggctgtggcagtggcggctgcgggggatccg *F tccacgtactttagcaccaccttccagacacccgataccgcctacgcgacgtacaacttc *F ttcggaagtccgtacaccacgcggtttagatacttctga *F >AE003746|GENSCAN_predicted_peptide_33|354_aa *F MEQCYNRGCGQLFDPQTNNDESCRHHPGEPFFHDAYKGWSCCNKKSVDFTEFLNIKGCTL *F AKHSNVKPPEPEKPVKDESDKDEVIEVRAPIREALPRPPIDSPLTVIQPTVAPALKDMVF *F AVKTPAAQKSSDAIEVGTTCKNNGCTYSFTGNSSDFGECTYHPGVPIFHEGMKFWSCCQK *F RTSDFSQFMAQKGCTYGEHKWVKENDDKKVVQCRYDWHQTATNVVMAIYAKKYDYSQSVI *F ELNPIRLHVNLVFPEQDNARFDLDLELRGIVNVSNASAHMYGTKVEIKLPKLEPGSWSNL *F NFPNKKLPVVKKSQVEEKKKQEESDEEFFDLDDIKAETSFRLSEMSMQSPNNLD *F >AE003746|GENSCAN_predicted_CDS_33|1065_bp *F atggaacaatgctataacagaggctgtggccaactcttcgatccgcagaccaacaatgat *F gaatcttgtcggcaccatccgggtgaacctttcttccacgacgcctataagggttggtcc *F tgctgcaacaagaagtcggtcgacttcaccgagttcctcaacatcaagggctgcaccttg *F gcaaagcactcgaatgtgaagccaccagagccggagaaacctgttaaagatgagtccgac *F aaggatgaggtaattgaggtgcgggcacccatccgggaagccctgccgcgtccgcccatt *F gattcgccgctaaccgttatacagcccaccgtggctcctgccctgaaagacatggttttt *F gcagtcaaaacgccggctgctcaaaagtccagcgacgccattgaggtgggaaccacttgc *F aagaataacggctgcacctactcctttacgggtaacagcagcgactttggcgagtgcacc *F taccatccgggcgtgccaatctttcacgagggcatgaaattctggtcctgctgccagaaa *F cgaacctcggacttttcccagtttatggcgcaaaagggctgtacctacggtgagcacaaa *F tgggtcaaggagaacgacgacaaaaaggttgtgcaatgtcgctacgactggcaccagacg *F gccaccaatgtggtgatggccatttatgctaaaaaatacgattacagtcagagcgtgata *F gagctgaaccccatcaggctgcacgtcaacctggtatttcccgagcaggacaacgccagg *F tttgacctggacctggaattgcgcggaattgtgaatgtgagcaatgcaagcgcgcatatg *F tatggcaccaaagtggagattaaactgcccaagctggagcccggctcttggtccaaccta *F aattttcccaacaagaaactgccagtggttaagaaaagccaggtggaagagaagaaaaag *F caggaggagagcgatgaggagttcttcgacctggatgacattaaagcggagaccagtttc *F cgtctttccgaaatgagcatgcaaagcccaaacaacttagattaa *F >AE003746|GENSCAN_predicted_peptide_34|262_aa *F MSRNYKEDQTNEVEALDSIYCGDMESKYHGTSLKSISNNCFCSVLATEPHHKFQIPIATE *F EYSSEEPEKGLACKLVFTFTATYPDGAPVVEIEEPENFEDMFETRLLEHLQKTIEENLGM *F EMIFSLVSSAQEWLNERWDEHKFHQEELREQKLREIEEEERKKFEGTRVTVESFLKWKLE *F FEESTGIAAKREKNNVSKKQTGRELFMCDNTLNDSDIKFLLEAGENIENVKIDETLFQDI *F GELDLDDDDDEDWVPGADDDDD *F >AE003746|GENSCAN_predicted_CDS_34|789_bp *F atgagccgcaactacaaggaagatcagaccaacgaggtcgaggcgttggactccatatac *F tgtggcgatatggagagtaagtaccacggcacttccttgaaatccatatccaataattgc *F ttctgttcagttctcgccacggagccgcaccacaaattccagattccaattgccacggag *F gagtacagttcggaggagcccgagaaaggtcttgcctgcaaactggtcttcacattcaca *F gctacctatccggatggagcacccgtggtggaaatcgaggagccagaaaactttgaggac *F atgtttgaaacgcgcctcctggaacacctgcaaaagacaattgaggagaacctgggcatg *F gagatgatcttttcgctggtaagcagcgcccaggagtggctgaacgagcggtgggatgaa *F cacaagttccaccaggaggaactgcgagagcagaagctgcgggaaatcgaagaggaggaa *F cgcaagaagtttgagggcacccgtgtgacggtggagtccttcctcaaatggaagctcgaa *F ttcgaggagagcaccggcattgccgccaagcgggagaagaacaatgtgtccaagaagcag *F accggacgcgaactctttatgtgcgacaacacgctcaacgattcggatatcaagttcctc *F ctggaggcgggtgaaaacattgaaaatgtcaaaatcgatgagacgctgttccaggatatt *F ggcgaattggatttggatgacgatgatgatgaggattgggtgcccggggcggacgacgac *F gacgattaa *F >AE003746|GENSCAN_predicted_peptide_35|1140_aa *F MERSRRGRIRRMPSPGSESDGSTAEPTRKRSKQQFVAEAEPDFVEEETEKYANASTSQTA *F RSRNKQARQTTLNMSQRSVNFNLTSELSIPNAFDRCGKVISMRLTNFMCHSNLFIEFGPN *F INFLVGNNGSGKSAVITALALGLTSSARATNRASSIQKLIKNGEVSATISITLSNSGLRP *F FKADIFGPHLTVVRQIRHSSSTYDLQDARGKSVSKKVSDIRRMLLCFGINVENPIFVLNQ *F EAAREFLKELEPASNYKLLMKATQLDVCTSSLTECHALRRHFTQELEQLEKKKEMMIKHI *F AAEEEKLSILEDKEMVKENLQQCKTKLAWMAVTSYQNELNNLEHSIKLIENKKASLEQTT *F SKKESTQATMNQKLKEFEASKNQILATQKFQDERLKTAKKAVQDLLLEASQVKAKIGNAE *F RRMREDQRSYDECEKLIGNYHADFNRVNEQREENANKIEMLKKQVVKSEEIIAQLRAEQQ *F EIKRDITSVQERLDAVKNGRIQLHKSKQNISWEIEALSRNKSNKLSVYGEQTIQVVHALR *F TQYAGSNMHRMPRGPLGQYISAPNPKYRDLIENQLMHCLRSFIVGSDRERQSLRALLQNK *F FQGGNMPTIITSPFTDRVYDVSRNKVQPTTPNTTVLIDEISCDDPVVMNYLIDILRIETV *F LVTESKEIAEFLTSDTENVPPNLTRVLVPNLGLEYIPSPNYAVYSTRITPARYIQKNVDD *F RIRQLQMEQSDLQEKEPSLEIDYMQHKKVLENTQKVISQKSTMIGQHQSRNQKAMQKIME *F LQNFDYQELPEYDRLKSHLADSGEKIEKCRLEREMLQEKLLSIQHRQTELESTEAEERRA *F LEGINKKLTALDTEAGEVESKMRSLDLHYEENTRRFQKTLQLERKMLGEKETVLSELEKA *F RTEAEKLGEFIATTQTEEKIREAISRYKSKIKQVEELNYNPEELERGLAELRDELELQSR *F HLAVVDSVVKKLRMAYHQRAQLFQRSRHHYFTMVQFQFEVYIGLLETYYILTNAFIYFSQ *F QALAMRQFKVSFETSDKEKTWKINVFPPSGNETSNTRSLSGGERSFTTVSLLKGLWSTSD *F HPFYFLDEYDVFTDEVNRKFITEILIGEGLEWLSRQYCFLTPQDTKVEASNLITVHKYEN *F >AE003746|GENSCAN_predicted_CDS_35|3423_bp *F atggagcggagtcgcagaggaaggattcggcggatgccttcgcccggatctgaaagcgat *F ggctcaacggcagaacccactcgcaagaggagcaaacagcagttcgtggcggaggcggag *F ccggattttgtcgaagaagagacagagaaatatgcgaacgcgtctacttcgcagacggca *F aggtctcggaacaagcaggcgcgccaaacaactttaaacatgtcccagcgctccgtgaac *F tttaatctgacgtcagagctatcgataccgaacgccttcgatcgctgcggcaaagtaatt *F tccatgcgtctcacgaacttcatgtgccactccaatctgtttattgagtttgggcccaat *F attaacttcctggttggcaataatggcagtggcaagagcgctgtaatcacggcactggct *F ttgggtctgaccagcagcgctagggcaaccaacagggccagcagtatacagaagttaatc *F aagaacggcgaagttagtgccaccatctccataacactgtccaattcgggattgcggccc *F ttcaaagcggacatcttcgggccccaccttaccgtggtgcgtcaaatacgccactcctcc *F tcgacgtacgatcttcaggacgctcgaggtaaaagcgtctcgaagaaagtgtccgatatt *F aggcgcatgctgctctgcttcggcatcaatgtggagaatccgatttttgtgctgaatcag *F gaggcggcgagggagtttctaaaagaattggagccagcatcgaattacaaactgttgatg *F aaagcaactcaactggatgtttgcaccagcagtctaactgagtgccatgccctgcgacgt *F catttcacccaagaactggaacaattggaaaagaaaaaagaaatgatgataaagcacatt *F gccgcggaggaggaaaagctgtcgattcttgaggataaggaaatggtcaaggaaaaccta *F cagcagtgcaaaacaaagctggcatggatggccgtgactagttaccaaaatgagctcaat *F aatctagagcattcaattaaactgattgaaaacaagaaggccagtctggagcagacaaca *F tccaaaaaggagagtacgcaagccaccatgaaccaaaaattgaaagagtttgaggcttct *F aaaaatcaaatattagcaacccaaaagttccaggatgaaaggcttaaaaccgctaagaaa *F gcagtacaggatctgcttctagaagccagccaagtcaaagccaagatagggaacgcagaa *F cggcgcatgcgagaggatcagcgttcgtatgatgaatgtgaaaagctgataggaaactat *F catgccgactttaatcgggttaatgagcagcgggaagaaaacgctaacaagattgagatg *F ttaaagaaacaagtagtcaaaagcgaggagatcatcgcccagttgcgagcagagcagcag *F gagattaaacgagatataacctcagtccaggaaaggttggacgctgtaaaaaatggaaga *F atacagctgcataaatcaaagcaaaacatcagttgggagatagaagctctgtcccgtaac *F aagtccaacaaactgtccgtgtacggtgagcaaacaatacaggttgttcatgcactgcga *F actcagtatgccggctccaatatgcatagaatgcctcgcggcccgctgggccagtatatc *F agtgcgcccaatccaaagtaccgcgacctcatcgagaaccagctcatgcactgcctgcgt *F tcctttatcgttggctcagaccgcgagcgccagtcgctgcgggcgttgctgcaaaacaag *F ttccaaggtggcaatatgcccactattataaccagtccgtttacggatcgggtttacgac *F gtgtctaggaataaggtgcaacccactactccaaataccacagttctaatcgatgaaatc *F agctgcgatgatcctgtggtaatgaactaccttattgatattctgcgtatcgaaacggtt *F cttgtgacggagtccaaggaaattgccgaatttctcacctccgacactgagaatgtgccg *F cccaatctaacgcgtgtgctagtgccaaacctgggactggagtacataccatctcccaac *F tatgccgtctactcgactagaataacacccgcccgctatattcagaaaaacgttgatgat *F cggatacgacagcttcaaatggagcaaagcgatcttcaggaaaaggagccttctttagaa *F atagactacatgcaacacaaaaaggtactggaaaacacccaaaaagtgatttcgcagaag *F agtactatgattggtcagcatcaatcaaggaatcagaaggcgatgcagaagataatggag *F ctgcaaaattttgactatcaagagctaccggagtatgatcgtttgaaatctcatttagct *F gatagcggcgagaaaattgagaaatgtaggctagaacgggaaatgctgcaggaaaaactt *F cttagcatccaacatcgccagacagaacttgagtcaactgaagccgaagagaggcgagcc *F cttgaaggtattaacaaaaagcttaccgcactggacaccgaagctggcgaagtcgaaagc *F aagatgcgaagcctggacctccactatgaagaaaacacacgtagattccagaaaacgttg *F cagttggagaggaaaatgcttggcgagaaggaaaccgtgctaagcgaattggaaaaggct *F cgcaccgaggccgagaaattgggagagttcatagcgacgacgcaaacggaggagaaaata *F cgcgaggcaatcagtcgctacaaatcaaagatcaaacaggtggaggagctgaactacaat *F cctgaagagctggagagagggctggcggaattgcgagacgaattggaacttcaatctcgg *F cacctggccgtggttgactccgtggtcaagaagctgcgcatggcctaccatcagcgagct *F caacttttccagcgatcgcgacaccattacttcacaatggttcagtttcagtttgaggta *F tatattgggttgttggaaacctattacattcttaccaatgcttttatttacttttcacag *F caagctcttgctatgcgacaatttaaagttagtttcgagaccagtgacaaggagaagacg *F tggaagattaacgtatttccgcccagtgggaatgagacttccaataccaggagtttatcg *F ggcggtgaacgatcgttcacgacagtttccttgctgaaaggactttggagcacttcagat *F catccgttctactttttggacgaatacgatgtgtttacagatgaagtgaaccggaagttt *F attacggaaattctaatcggcgagggtttggagtggttatcgcgtcagtattgttttctg *F acgccccaggatacgaaggtcgaggctagcaatctgatcactgtgcacaagtatgaaaac *F taa *F >AE003746|GENSCAN_predicted_peptide_36|903_aa *F MSSSDDDWFDQDENKLLQGLEKSLKSLELQKNEEYIECPPSERKCPPSEVGEYVMQHTRF *F SLTELTNALKMPAIDMFLYFLSDKRDLFENQVLATDNVKRVGLFVDVLWSLCELELGGFD *F EVFLSAFSRQTALLDKIKNLLQAKAAVAKCDAESALILSHSKWMLLRAHKHGLLSHQGYE *F LVELYKKLAPSFKSDMIDGLEAFTGNFSHNVKGLIYPTLETLLGKDATKAPNEEEDEGLV *F SDKVVKYVNALRNLLREDFLAPLVEFVQQLRSGTDVDELKQQGLLWSDVHLTLNPQFANA *F QRHSLVFLKVQFTKESKNAYKTWLNSIKSGTLLCLTTSLAFDDLILASVGYTEPEKLKED *F CLSVQIVKQYNIGNAYNRPLIMFQAPVFFEPYLRVHNYLSTCSTEKFPMGRYIVDGQMEI *F PPPAYMKPGVKLSFNMKPFTLDKLPEDLHLNESQKTAFKEALCREFSIIQGPPGTGKTHL *F SVQLVNSLIQNAKALGTGPIIVLTYTNNSLDKFLVKISRYTQEILRFGNQSRDPQISKFN *F LSTTIKPELVPPRLKRIWWLVNCEYKEKFRNLQGLYANFDGSEESYQDTLAAQEKLNQVA *F ERIETLRMVFQFFLAREKDLLAMTTTCAARHNFLFRLLQSKCVLFEEAAEIQEAHIVACL *F TPHTEHVILVGDHKQLQPFSGSRKVPQISLFERLIVAGLPFSRLNLQYRMRSCISELLVP *F SIYDELLCSESVKEYEDIRLMSKNLYFVQHNQPEHCMSDMSIGNLYEAGVLAKLTEFLIQ *F KAQYKHSDIVILSPYNGQIECIKNALPQNYRSTVQVASVDSFQGLEANIVLLSLVRSNIS *F GRIGFLRQANRVCVALSRARWALYIVGNVTILKDTFPKIWNPIVKRLKENNAIGEAFPTI *F TST *F >AE003746|GENSCAN_predicted_CDS_36|2712_bp *F atgtcaagttcagatgacgattggtttgaccaggatgagaacaaactgttgcagggcctg *F gagaagtccttgaagtccctggagctgcagaagaatgaggagtacatcgaatgcccccca *F tctgagcgtaaatgccccccatctgaggtgggggagtacgtgatgcaacatacacggttc *F tccctgaccgagttaacaaatgccttaaaaatgccagccatcgacatgttcttatacttt *F ttgtccgataagcgagatctcttcgagaatcaagtgttggccactgacaatgtgaaacga *F gttggcctgttcgtggatgtcctgtggtcgctctgtgaactcgaattgggcggattcgat *F gaagtctttctgtccgcattcagccggcagacggcgcttctggacaagatcaagaatctt *F ttgcaggccaaagccgctgtggcaaaatgcgatgcggagtcggcactgatattaagccat *F agtaagtggatgcttctacgagcccataagcatggcctccttagtcaccagggctacgaa *F ttggtggaactttataagaaattggcaccttcctttaaaagcgacatgattgatggtctt *F gaagcattcaccggtaacttttcacataacgtcaagggcctaatttatccaacgctggag *F acgttactgggcaaagatgcaactaaggctcccaatgaagaagaggatgagggcttggtg *F tccgacaaagtagtcaaatatgtgaatgcactgcgaaatttactaagggaagatttttta *F gcaccactagttgagtttgtgcaacagctgcgcagcggaacggatgtcgatgagttgaag *F caacagggccttctgtggtccgatgtgcatctgactttaaatccacagtttgccaacgct *F cagcgtcatagccttgtttttttgaaggttcaatttactaaagaatccaagaatgcctat *F aagacttggctgaattctatcaaatctgggactctgctctgtcttaccacgagtctggcc *F tttgatgatttaattctggcttccgttggctacactgagccagaaaaactaaaggaggat *F tgcctaagtgtgcagattgtcaagcagtataatattggaaacgcctacaaccgaccactg *F atcatgttccaggcgcccgtgtttttcgagccttatctcagagttcacaattacctgagc *F acctgcagcacagagaagtttcccatgggtcgctatattgtagacggccagatggagata *F ccgccgccagcttacatgaaaccaggagttaagcttagtttcaatatgaagcccttcacg *F ctggacaaactaccggaagatctgcacttaaatgagagtcagaaaactgcattcaaggaa *F gctttatgcagggagtttagcatcattcaaggacctccaggcaccggaaaaacacacctt *F tcagtgcagttggttaacagtttgatacagaatgctaaagccctgggcacgggacccatc *F attgtgctgacctataccaacaattcgctggacaaattcctggtaaaaatttcgcggtac *F acccaagaaattcttcgctttggtaatcagtcgcgagatccgcaaatatcaaagtttaat *F ttgagtaccacgatcaagccagaattggttccaccgcgcctgaagcgaatttggtggcta *F gtcaattgtgagtacaaggaaaaattccggaacctacaaggcctgtacgcaaactttgat *F ggcagcgaggaaagctaccaggacactctggcggctcaggagaagctaaatcaggttgcc *F gagcgtatcgaaacactgcgcatggttttccagttctttctagcaagggagaaggacctg *F ttggccatgaccaccacgtgcgcggctcgccataactttctgttcaggttgctacaatca *F aagtgcgtcctctttgaggaggcagccgagatccaggaggcacacatcgtggcatgccta *F acgccgcacacagagcacgtgatcctcgtcggcgatcacaagcagctgcaacctttcagt *F ggcagcaggaaggtaccacagatctcgctctttgaacgactcattgtggcggggctgcca *F ttttcacggctcaatctgcagtaccgcatgcgatcctgtatttctgaactccttgtgccc *F agcatttatgacgagctgctctgttcggagtcagtgaaggaatacgaggacatccgcctg *F atgtctaagaacttgtacttcgttcagcacaaccaacctgagcactgcatgtctgatatg *F tccattgggaatctttatgaagctggagtgttggctaaattaactgagttcctaatccag *F aaggcccaatataagcacagcgatattgtgatattatccccttacaatggccaaatagag *F tgtatcaagaacgcgcttccccaaaactatcgctcgactgtgcaagtggccagtgtggat *F agtttccaaggccttgaggccaatatagtgctgctctcgctggtgcgcagcaatatatcc *F ggccgaattggcttccttcgccaagcaaatcgagtgtgcgtggctctttctcgagcccgc *F tgggccctgtacatcgtcggtaatgtgacgattttgaaggataccttcccaaagatttgg *F aatccaattgtcaagcgcttgaaagagaataatgccatcggagaggcatttccgaccata *F accagtacctga *F >AE003746|GENSCAN_predicted_peptide_37|1270_aa *F MSRNKDKYDSANRRQQIFLSQEDIAAGKKTNWSGLEITGCVRNISPSLWEFEHLTALYLN *F DNQLLRLPADVGMLTSLRTLDLSSNKLRSLPAELGELIQLRELLLNNNFLRVLPYEIGKL *F FHLVILGLMGNPLQKEFMNIYNEPNGTQKLLTYMLDNLSCESPPCLSKSLSMAPESAAPP *F PPPPNYASLRYHQHPLSHWPGSQHPAPVYRLHHGPPSAPPPNWAAQSAGQPPQPVGYGVV *F NQQRINECAGIPLISNASSHLSPAQRQRLLRKSALKTCPTWPPGGATMSQLAVHAQGMPL *F LQQNGKHHLQQQQMQHHQPLHHHHPVTNNWSGYQPMSSQPAFKLADKSRALRYQNSAPPV *F LSSQEKTPNNAAGQDQATSPAETCLPRIIKPRKRRKKDRKPGNGVLLKIEADMQPKMSNP *F LDGYTANAGASYHLPSGILQHDHTHGVCFCRECDPLRSLWDYPLRRSLSDASSSEPGGGS *F RESSSSTSSTHSDSSCDSSICSQSLPSSLALQEETTEEFAPITGDSSRAEKVGVIGSQRS *F HPSAVATPSQCLSDDSGYGDILSGINIANDLFGNCWRGGKLGNASSSISAQAETLLDASL *F NEISRKLIETCNAVDQAESESGSGFGSGAASDSGLDSAGSHNCGSGLVFNFEHLNLTDAT *F PTALDFLVDCNNNSSSTTTAAAATATTIGNGNSLGLMWHGGRQSSGGGSHDDSQVAAAKL *F GPDSRATPTRLILGTVASERSPNRWPSERLGSTFPLPLYRCIAERCTQFVCKPWRPLLRH *F NVVSNGPVTATNSRNGGAGVARKNFKPHHPHHLHHQHHLNHPHQPPHHPHHQRHPQQQQL *F QYRSYYAYLLQPSQIGFATPQHAPLSWRSTCPRRQASNATSSRSITVNPPPQRPWLPLAK *F PNKTRPACIFTVMCYNVLCDKYATRQMYGYCPSWALCWEYRKKSIIDEIRHYAADIISLQ *F EIETEQFYHFFLPELKNDGYEGIFSPKSRAKTMSELERKYVDGCAIFFRASKFTLIKESL *F IEFNQLAMANAEGSDNMLNRVMPKDNIGLAALLKVKENAWEPMSEVTQISQPLLVCTAHI *F HWDPEFCDVKLIQTMMLSNELKTIIDEASHSFRPGHKNDSNAVQLLLCGDFNSLPDSGVV *F EFLGKGRVSMDHLDFKDMGYKSCLQRLLSNDTNEFTHSFKLASAYNEDIMPHTNYTFDFK *F GIIDYIFYTKTGMVPLGLLGPVSNDWLRENKVVGCPHPHIPSDHFPLLVELELMHTASQQ *F APPNGLINRR *F >AE003746|GENSCAN_predicted_CDS_37|3813_bp *F atgtctcgtaacaaagacaaatacgacagcgccaatcggcggcagcagatctttctgtcg *F caggaggacatagctgcgggcaagaagacgaactggagtggcctagagattactggctgc *F gtccgcaatatcagcccgtcgctgtgggaatttgagcatctaaccgccctctacctgaac *F gacaaccagctgctgcgattgcccgcagacgttggcatgctgaccagcctccgaactttg *F gacctgtccagcaataagctaagaagtcttcccgcagagctcggcgagctcatccagttg *F cgggagctgttgctgaacaacaactttctgcgcgtactgccttacgagatcggcaagctg *F ttccacctcgtcatactcggcctcatgggcaatccgctgcaaaaggagttcatgaacatc *F tacaacgaaccgaacggcacgcagaaactgctcacctacatgctggacaacttgtcatgt *F gagtcccccccatgtctatctaaatccctgagtatggcgccagaatcagcagcaccacca *F ccaccgccgcccaactacgcgtccttgcgataccaccagcatccgttgagccattggccg *F ggtagccagcatccagctcctgtgtacaggttgcatcatggtccgccgtctgcaccaccg *F cccaattgggcagctcagtcggctggccaaccacctcaaccagttggctatggagtggtt *F aatcagcagcggatcaatgagtgcgccggcataccgctcatctcgaatgcctccagtcac *F ttgtcgccagcccagcgacagagattgctgaggaaatccgcccttaaaacatgtccaact *F tggccacctggaggggccactatgtcccagctggctgtgcatgcccagggcatgccgctg *F ctccagcagaatggcaagcaccatctccagcaacagcagatgcagcaccaccagccacta *F catcaccatcatcctgtgaccaacaactggagtggctaccagccgatgagcagtcagcct *F gctttcaagttggctgacaaatctcgagcccttcgctatcagaattcggcaccaccggtg *F ctcagtagccaagagaagacgcctaacaatgctgctggccaggatcaggccacctcgcca *F gcggagacctgtctgccgaggatcattaaacccaggaagcgacgaaagaaggatcgaaaa *F ccgggcaatggggttttgctcaaaattgaggcggatatgcaaccgaagatgtccaatccg *F ctagacggttacaccgcgaatgcgggagcatcttaccatctaccatctggaatattgcag *F catgatcatacgcacggagtctgcttctgccgagaatgtgacccacttcgttcgctttgg *F gattacccgctccggcgatcgctttccgatgcctcttccagcgagccaggaggcggaagc *F cgagagtcctcctcctcaacgtcatccactcactcggacagctcctgcgatagctccatt *F tgtagccagagcctgcccagctcattggccctgcaggaggagaccaccgaggaattcgct *F cccattaccggcgatagcagccgggcggaaaaagtgggagtaatcggatcccagcggagt *F catccgagcgcagtggccacaccttcgcaatgcctgagcgatgattccggctacggggat *F atcctgagtggaatcaacattgccaacgatctgtttggaaattgttggcgaggtgggaag *F ctgggcaacgcctcctcctcgatttccgcccaggcggagacgctgctcgatgcgagcctc *F aatgagatatcacgcaagctgatcgagacctgcaatgcagtagatcaggcggaatcggaa *F tcgggatcgggatttggatcgggtgcagccagcgacagtggcttggatagcgctggcagc *F cacaactgcggatcgggccttgtctttaactttgagcatttgaatctgacggatgcgacg *F cccacagccttggattttctcgtggattgcaacaacaatagcagcagcaccaccaccgcc *F gccgccgccaccgccactaccattggcaacggcaacagtttgggtttgatgtggcacggg *F ggaaggcagtctagtggaggtggcagccacgatgacagccaagtggcagctgccaaactg *F ggcccagacagccgggccacacccaccaggctgatattgggcacggtggcgtcagagagg *F agtccgaaccgctggccatcggagagattaggttccacattcccactgccactgtatcgc *F tgtatcgccgagcggtgcacccagttcgtttgcaagccgtggcgccctttgctgcggcac *F aatgttgtaagcaacggcccagtcacggctaccaattcgagaaacggtggcgcgggtgtg *F gctcgcaagaatttcaaaccgcaccacccgcaccacctgcaccaccagcaccatctgaac *F cacccacatcaaccgccgcatcatccgcatcatcagcgccatccgcagcaacagcagctc *F caatatcgttcctattatgcttacctgctgcagccgtcgcagatcggtttcgccacaccg *F cagcatgctcctctcagctggaggagcacatgtcccagacggcaagcaagcaatgctacc *F tcatcacgtagtattaccgtgaatccaccgccccagaggccctggctgccactggccaag *F cccaacaaaacgcgaccagcctgcatttttacggtcatgtgctataatgtgctctgcgac *F aagtacgcgacgcgacaaatgtacggatactgtccgtcgtgggcgctatgctgggagtac *F cgaaaaaagtcgattatcgacgagatacggcactatgcagcggacattatcagtctgcag *F gagatcgaaacggagcaattctatcacttcttcctgccggaactcaagaacgatgggtac *F gagggaatcttctcaccgaagtcgcgtgctaagactatgtccgagctggagaggaagtac *F gtcgatggctgtgcgatattcttcagggcgtccaagtttacgctgatcaaggaatcattg *F atcgagttcaatcagctggcaatggccaatgccgagggctccgacaacatgctgaaccgc *F gtaatgcctaaggataacatcggtctggccgcactgctcaaggtgaaggagaacgcatgg *F gagccgatgtccgaggtgacgcagatctcgcagccgctgctcgtctgcacggcgcacata *F cactgggaccctgagttctgcgacgtcaagctcatccagacgatgatgcttagcaatgag *F ttgaagacgatcatcgacgaggcgagccacagtttccgacctggtcacaagaacgactcc *F aatgctgtccagctgctgctgtgcggtgacttcaactcgctacccgattcaggcgttgtg *F gagtttctcggcaagggccgcgtttccatggatcatttggacttcaaggacatgggctac *F aagtcctgcctgcagcggctgctctcgaacgacaccaacgagtttacgcactcgttcaag *F ctagcctccgcctacaacgaggacataatgccgcacaccaactatacgttcgattttaag *F ggcatcatcgactacattttctacacgaagacgggcatggtgccgctgggcctgctgggt *F cctgtctccaatgattggctgcgcgagaataaggttgttggatgcccacatccgcatata *F ccctctgatcacttcccactgctggtcgagctagagctgatgcatacggctagccaacag *F gcgcctcctaacgggctgatcaatcgccggtag *F >AE003746|GENSCAN_predicted_peptide_38|337_aa *F MSGTQMSAFLRKYLADEDKKIRAQFKESDPNNKLILWMHEKTRITEEDLARPYTEDEVKE *F LCLRTKVKVDMTAWNCLWEAKKRFEAKGRFVNKSERFINRMYMKAVRRKMVQPYPEEFVA *F QRREIVAAETKKQNISRLDRWQKKKSQNLSAPESSPDAHASSNDAVQSHEDQANTNLSSL *F SQMNFQVEAMAPPGVSSSDLSGIGDDEDEQQQSGFQDENINRPETEINENSVRCDPINLG *F RMRTGCINSQANNSFRNTESDPDYYMFGTQLSTLVRPTSTQEPDDQVNCPETEMNESWVR *F CDQINSESLSIGPSIDSEGTITFQNTESEPIDVTSIA *F >AE003746|GENSCAN_predicted_CDS_38|1014_bp *F atgtcggggacgcaaatgtctgccttcctgagaaagtatctagccgacgaggacaaaaaa *F attcgtgcgcaattcaaagaaagcgatcctaacaacaaattgattctatggatgcacgaa *F aaaacgagaataaccgaggaggacttggcacgcccatacaccgaggacgaggtaaaggag *F ctttgtctacgcaccaaagtgaaggttgatatgaccgcttggaattgtctgtgggaagcc *F aaaaagaggtttgaagcaaaaggacgttttgtgaacaagtctgagagattcatcaaccga *F atgtatatgaaagcggtgcgcagaaagatggtccaaccgtatccggaggagtttgtggcc *F cagcgaagagaaatagttgcagccgagactaagaagcagaacatcagccgattggataga *F tggcaaaagaaaaagagtcaaaacctatcagcaccagaatcctctccagacgctcatgca *F tcttctaatgacgcggtgcagagccacgaggaccaagcaaacacaaatcttagttcactg *F tctcaaatgaactttcaagtggaagcgatggctccgccaggcgtgtcgtcatctgatctt *F agcggcatcggagacgatgaggacgaacagcagcagtcaggatttcaggatgagaacatc *F aaccgtccagagacagagattaatgagaattcggtgagatgtgatccaattaatttagga *F aggatgcggactggatgtataaattcgcaagcaaataacagtttccggaatacggagtct *F gatccggactactatatgtttggcacccaattgagcacattagtacgacccacgtcgact *F caagagcctgacgatcaggtaaactgtccagagacagagatgaatgagagctgggtaagg *F tgtgatcaaattaattcggaaagcttgtcgattggaccgtcaattgattcggaaggaact *F atcacttttcaaaatacagaatctgagccgatcgacgtcacctcaatagcctga *F >AE003746|GENSCAN_predicted_peptide_39|968_aa *F MLQYKSAASAATSAPPATPLAAGGSSKATIPPNGASAAASQTQVHGAPGTPTMQQIINIH *F QMPPQFAGGAVAGNGQNAGMPQNMFQIVQPMPMQTVNIDGQEAIFIPNLNAQLATAQAVN *F FNGQQAFITPNGQILRAPQMAANPAASNCIQLQQLNGLGQEQTQLITIPGTNIQIPVTNL *F IQQQQQAQQVHQGTVQQQAQGTNASGANGSGVTNSGTAGQLPGSITIPGTNLQIPTSVAA *F ANGLLGNISNISNLLGGGQSIKLENGQLQMRPQLVQFPAPAMPQQQQTVAVQIPVQTANG *F QTIYQTVHVPVQAAATSSGGLQNLMQAQSLQMPSASQMQIIPQFSQIAQIVTPNGQIQQV *F QLAMPYPQLPPNANIIHIQNPHQQQQQQVQQQQQQQQAQQQQQAQQQQAQQQQQQQVQAQ *F HQQLLQAISDASAGGQLPPNQPITITNAQGQQLTVIPAQLRPNAPTAPTPAPAGVPTPMQ *F MPNLQALPIQNIPGLGQVQIIHANQLPPNLPANFQQVLTQLPMSHPQVQTQGQVQVMPKQ *F EPQSPTQMITSIKQEPPDTFGPISATGNPPAPASTPNTASPQQQQIKFLHTESNSLSSLS *F IPASIQITALPQQATNTPNTPATTQPIPVSLPARSKVNAVTTSSTQITIAPTGGQVVSVT *F TQARGATASIRSTNTSTTTITTPSQSHLNMNISVASVGGAATGGGGGTATGEPKPRLKRV *F ACTCPNCTDGEKHSDKKRQHICHITGCHKVYGKTSHLRAHLRWHTGERPFVCSWAFCGKR *F FTRSDELQRHRRTHTGEKRFQCQECNKKFMRSDHLSKHIKTHFKSRSGVELIELSIKQET *F KGGNAPKSISTVNGIVTIEIPGGGSAAAGSGASSVAATVAGSTVTPGGATIVQLPTVEAS *F GGGDSFGDDEDDEEDDELTEEDDEDEELDEDDMDDCEDEEDDEDPELDSGDEVKMTIAVS *F EPGDNSSN *F >AE003746|GENSCAN_predicted_CDS_39|2907_bp *F atgctgcagtacaagagtgccgccagtgcggcgactagtgcgccacccgccacgcctttg *F gcggcaggcggttccagcaaggcgacgattccaccaaatggagctagtgcagcagcttca *F caaacgcaggttcacggtgctcccgggacaccaaccatgcagcagatcatcaacattcac *F caaatgcctcctcagtttgcaggcggagcggttgccgggaacggacagaacgctggcatg *F ccccaaaacatgttccagatcgtacagcccatgcccatgcaaacggtgaacattgatggc *F caggaggccatcttcataccaaacctcaatgcacaactggccaccgctcaggcggtcaac *F ttcaacgggcaacaggcctttatcacgcccaacggtcaaattctgcgggctccccagatg *F gcagctaatccggcagcctctaactgcattcagctgcaacagctgaacggcttgggccag *F gagcaaacccagctgatcaccatacccggcaccaacattcaaatacccgtcaccaatctc *F attcagcaacaacagcaggcacaacaagtgcaccagggtacagtgcaacagcaggcacaa *F ggaacaaatgcatctggagccaatggatcgggagttaccaatagtgggacagcgggtcag *F ctgccgggcagcatcaccataccgggcacaaacctgcagataccaacctcggtagcggcg *F gcgaatggattgctgggaaacatctccaatatctccaatttactaggcggcgggcaatct *F ataaagttggagaacggccaattgcagatgcggccgcaactggtgcagtttccggcgcca *F gccatgccgcagcagcaacaaacggttgctgtgcagattcccgttcagactgctaacgga *F cagaccatctaccagactgtacatgtgcccgtccaggcagcggcaacatcgagcggcgga *F ctgcaaaacctgatgcaggcgcaatccctgcagatgccctccgcctcgcagatgcaaatc *F atcccgcagttctcacagatagcccagattgttacgcccaacggtcagattcagcaggtg *F caactggcaatgccgtatcctcagctgcctccgaatgccaacattatacatatccagaat *F ccccaccagcagcagcaacaacaagtacaacaacaacaacagcagcagcaggcgcaacag *F cagcagcaagcgcaacagcaacaggcacagcagcagcaacaacaacaggtccaggcgcag *F caccagcagctccttcaggcgatcagcgatgcctcggcggggggacaactgccgcccaat *F cagcccatcaccattaccaatgcccagggccaacagctgaccgtgattcccgcccaacta *F cgcccaaatgcgcctaccgcacccactcctgctccagctggtgtgcccacaccaatgcag *F atgcccaatcttcaggctttacccatccagaacatcccaggcctgggtcaagtgcaaatc *F attcacgccaatcagctgcctcctaatctgccagccaacttccagcaagtactaacccaa *F ctacccatgtcgcatcctcaagtgcaaactcagggccaagttcaggtgatgcccaagcag *F gagccgcagagtccaacgcaaatgatcaccagcatcaagcaagagccgccggataccttt *F ggacccatttccgcaactggtaatcctccggcacctgcctctactccaaacacagcctca *F ccgcaacagcagcagatcaagttcctgcatacggaaagcaattccctgtccagcctgagc *F ataccagcctccattcagatcacagccctgccacagcaagcgacgaatacaccgaatacc *F ccagccacaacccaaccaattcccgtatcgttacctgcaagaagtaaggtcaacgctgtg *F accacgtcaagcacccagattacaattgcgccgactggtggccaagtggtgtccgtcacg *F acgcaggctaggggagcaactgccagcataaggagcacgaacaccagcacgacgactata *F acaacgccgtcacaaagccatctcaatatgaacataagtgtggccagcgtcggaggtgct *F gcaactggcggcggcggtggaacagcgactggagagcccaaaccacgcttaaagcgagta *F gcctgcacctgtcccaattgtacagatggcgaaaaacactcggacaagaagcgccagcat *F atatgccatataaccggctgccataaggtatacgggaaaacttcccatctaagggctcac *F ctgcgttggcatactggcgagcgaccatttgtctgctcctgggcgttttgcggcaagcgc *F ttcacccgctccgatgaactgcagcgccaccgacgaacgcacacgggagagaagcgtttc *F cagtgccaggagtgcaacaaaaagttcatgcgcagcgatcacctgtcgaagcacatcaag *F acgcactttaagagccgctctggcgtggagctaattgagctgagcatcaagcaggagacc *F aagggtggcaatgcaccgaaaagcattagcacggtgaacggcattgtgacgattgagatt *F ccgggcggcggttcagcggcggcgggcagtggagcctccagtgtggcggccacggttgca *F ggctcaactgtgacgccgggcggggccacgatcgttcagctgccgactgtggaggccagc *F ggcgggggtgatagtttcggcgatgatgaggacgacgaggaggatgatgaattgaccgaa *F gaggacgacgaagacgaggagctcgacgaggacgatatggatgattgcgaggatgaggag *F gatgacgaggatccagaactagacagtggcgatgaagtgaagatgacaattgcggtcagt *F gagcctggcgacaattcgtccaactag *F >AE003746|GENSCAN_predicted_peptide_40|364_aa *F MEPVPEEARGVGDDEGELDCLTHMIGALLLQKEPENPENGDSKETIWSGELEWEDAQILD *F QPKTLHTVQCKICSMVKEGQPEINTENWPNKLKTQLIPKKVLGKIGEQFLKDARMVVFRS *F SQGEVLNLLITAMSSGFAGCIHFPSNPNCNIKALILIYSRDHQALVGFIPNNEDSFSERL *F QEILQGAKRKPGVKTPKQPQPEEPPPVEEDAIINELLWTGSLNWSTQASLEEPSISHKLE *F CSVYIAIKNGDPGISAEDWPTDMPMVLMPSIYLGQFAGAFIKDSKLIILRSTPGEEHDSL *F ASSMSAGSCGCARFSSEVVCKVIMLLYSSPRNAFLGFIPRDQANFVKRLREVLDEHRQKA *F RNKE *F >AE003746|GENSCAN_predicted_CDS_40|1095_bp *F atggagccggttccagaggaagcccgaggagtgggtgatgacgaaggggaactcgattgt *F ctaacacatatgattggggcgttgcttttgcaaaaagaaccggaaaatcctgaaaatggt *F gactcaaaggaaacaatttggagtggagagctcgaatgggaggatgcacagatattggat *F caaccgaagactctgcacacggtccaatgcaaaatctgctccatggttaaagaaggtcag *F ccggagatcaacacggagaactggccaaacaagctgaaaacacaactgatacccaaaaaa *F gtgctgggtaaaattggcgaacaattcctaaaggatgccaggatggttgtattccgatcc *F agccaaggagaggtactcaatttattgatcacggcgatgagctctggtttcgctggttgc *F atccacttcccctccaatcccaactgcaacatcaaggctctaattctcatctactcgcgc *F gatcatcaagctttggttggcttcatacccaacaatgaggattcgtttagcgaacgactg *F caggagattcttcagggtgcaaaacgaaagccgggcgtcaaaacacccaagcagccacag *F ccagaagaaccgcctcctgtggaggaagatgccattataaatgaactcctatggactgga *F tcgctgaattggtcgacacaagcaagtttagaggagccaagcattagtcacaagcttgag *F tgttccgtgtacatagctataaaaaacggtgatcccgggatcagtgcggaggattggcca *F actgatatgcctatggttttgatgccttcaatttatcttggtcagttcgccggagcgttt *F ataaaggactcgaaacttataattctccgatcgacacctggagaagaacacgactcgcta *F gcatcatcaatgtccgccggcagttgtggctgtgcccgattttcctccgaggtcgtctgc *F aaggttatcatgctgctgtattcgtccccgaggaacgccttcctgggctttattccgaga *F gatcaagctaatttcgtcaagcgattgcgggaagtattagacgaacatcgccaaaaggcg *F agaaacaaagagtaa *F >AE003746|GENSCAN_predicted_peptide_41|179_aa *F MHTVLTRGNATVAYTLSVLACLTFSCFLSTVFLDYRTDANINTVRVLVKNVPDYGASREK *F HDLGFVTFDLQTNLTGIFNWNVKQLFLYLTAEYQTPANQLNQVVLWDKIILRGDNAVLDF *F KNMNTKYYFWDDGNGLKDNRNVSLYLSWNIIPNAGLLPSVQATGKHLFKFPADYATSSI *F >AE003746|GENSCAN_predicted_CDS_41|540_bp *F atgcacacggtgttaacccgaggaaacgccacggtggcatacacgctgagcgttctagct *F tgcctcaccttcagctgcttcctgtccaccgtctttcttgactaccgtaccgatgcaaac *F atcaacacggtcagagtgctggtgaagaatgtcccggattacggagcgtcccgggagaag *F cacgacttgggcttcgtgaccttcgatctgcaaacgaatctcacaggcatcttcaactgg *F aacgtgaagcagctgttcctgtatctcaccgccgagtaccagacaccggccaatcaactc *F aaccaggtggtactgtgggacaagattatcctgcgcggcgacaatgccgtattggacttc *F aagaacatgaacaccaagtactacttctgggacgacgggaatggcctgaaggacaaccgc *F aacgtatcgctatacctctcgtggaacatcattccgaacgcgggactccttccctccgtt *F caggccactggaaagcacctgttcaagttcccggccgactatgccacctcgtccatttag *F >AE003746|GENSCAN_predicted_peptide_42|3905_aa *F MNNDAKNHESDDLNVRSTAYFNQQTTTNQPKAPATSKNNTGSGSGSNNNNNNTNQNPNRQ *F LNHNLPRIAAARQSIAAALLKNSGRKILTAKNEPLTTTESSGVLTNTPLPSNSRLKVNNN *F NNTNNTAKMSGTSSSQSSATPTPPTASSSTTTTTTTNISTGGGGSGSSGGGGGSTTVIAN *F PASVTNTGAGSAAKFRAAVASAPSPALPATNAPANATAAAAIAAIATAPAPSSSSSSSSS *F SKKTRAAVAALKRQVALQQQQPVTGNAPNMTSKDSAHLKFATTTLLMGAAAAAADSNAGA *F ALGGSGAGGSGSSSSVGAVGGARMALNPAVDMANAAVLLKQKLKDAAAAASASASNRSAT *F SSMSSTASSLSSSAGIVNAISSALQNIITPDTDTDTEFYPQPVTTDLSESEEESVSEDDI *F PESDPDSCPHEGEVREDEDETEEESEDSDESEGEEEEEDEEEIDVLQDNDADDEEIDDED *F EEEDAPEVSSFLLDANNKRSSNISALLEAAANEKAPVLRHATHAIDETKQALTKMRCASS *F PRDKNSGFSRSLVAACTDNDVNTVKRLLCKGNVNLNDAAASTDDGESLLSMACSAGYYEL *F AQVLLAMSAAQVEDKGQKDSTPLMEAASAGHLDIVKLLLNHNADVNAHCATGNTPLMFAC *F AGGQVDVVKVLLKHGANVEEQNENGHTPLMEAASAGHVEVAKVLLEHGAGINTHSNEFKE *F SALTLACYKGHLDMVRFLLQAGADQEHKTDEMHTALMEASMDGHVEVARLLLDSGAQVNM *F PTDSFESPLTLAACGGHVELATLLIERGANIEEVNDEGYTPLMEAAREGHEEMVALLLSK *F GANINATTEETQETALTLACCGGFMEVAAFLIKEGANLELGASTPLMEASQEGHTDLVSF *F LLKKKANVHAETQTGDTALTHACENGHTDAAGVLLSYGAELEHESEGGRTPLMKACRAGH *F LCTVKFLIQKGANVNKQTTSNDHTALSLACAGGHQSVVELLLKNNADPFHKLKDNSTMLI *F EASKGGHTRVVELLFRYPNISPTENAASANVTQAAPTSNQPGPNQMRQKIMKQQLQHQLQ *F QLNAPPGLHELSEAARASNQQHFHQQQFSSAGNGSSNIVAMGTGDFLDAGELQLTATAGM *F SAGAGTSTTGSETGMEEYGEVGGIDLTTLGAQQQEGLIAKSRLFHLQQQQQQQQQQQQQQ *F QQQQQQQQQQQQQQQQPPAAGQHQLVPCKHFDLDMEHINSLQPPQKAPPAPPVLFHTVCQ *F QPVMQQQQQQLQPGQLKLKAMLPNRNRALKTAEVVEFIDCPVDQQQPGEQVRTQPLGEDG *F KTPQFACAGEDPRLQRRRGFMPELKKGELPPESSSSDPNELALKGSYSLYNSLQSGADNN *F QPVPTALDNSACAQIPARNSGGAITHSSEVLQSTAISDRPKVKATNKNNRKQAAAAAAAA *F AAAAAAAAAAAQHAQQVLPNPMVSIYNNLHLQHLQHPHLQFQQQLQLHHQRVAGLDNAAA *F AAAAAASSANMAYSISPASPLPSPTGSGNYVDQQLQQQSMDVALQRKTAMDDFRGMLETA *F VNGPRGRKDLALNTPQLNFFKDGWHMVGVHNFFGDQPKSPTETPPEMEETTMSSPTEADR *F LGSEPRAEMKNLATLCSAAAAAAAVAAVNKDQVEISSDLESECEDDAEGGAGADCEENTL *F PPEPIELAAALREDGIIVEEEEDDEEEDDDDEEQDTNSGEVDKLNYDDEDAEVDNDGEVD *F YIDEDEGGGEGEEEEDDADDDEFFLDEPDSDQGTGNNNNNSKSGASSLPLKQRKMATRLE *F NLILNSQTVCDFPPELSNSELVHVLPQISNLKAAANSNAALNSVLQQQLAAASAAAAHAK *F ASVVHQKQQHGEGDQQCGDVPQDAQRQANLVLLDYPMQQNIQLEQRLLDAEEMHLQQHQQ *F TPLSLLPFTDEQQQQLHHQALSNASDFQQHQQLALENDPELKQQLQQNSNARIIKAVAAQ *F HQQQPPTNFVYNVESGDKNAPPVQLLFQLPPHMAQHQAQQQQGVGEPLTEQQQQQLHAEQ *F AHLFQHRTGGQRPPTQSELEQVAQELLLQRSGQVPAGAPVVGVQAIPLKQKHFNLHPPPC *F PPTCVQHQASQQQQMQQNELSIWPMATPTPAPSSGVSSTKSMPGGIAKKAIDKQSRKERR *F CVVRQTPAGIQENTKLHLQPQVATAQQQFLVQNQLAVATTVSLDKTIEIDSETESNHDTA *F LTLACAGGHEELVELLINRGANIEHRDKKGFTPLILAATAGHDKVVDILLKHSAELEAQS *F ERTKDTPLSLACSGGRYEVVELLLSVGANKEHRNVSDYTPLSLAASGGYVNIIKLLLSHG *F AEINSRTGSKLGISPLMLAAMNGHTPAVKLLLDQGSDINAQIETNRNTALTLACFQGRHE *F VVSLLLDRRANVEHRAKTGLTPLMEAASGGYIEVGRVLLDKGADVNAAPVPTSRDTALTI *F AADKGHQKFVELLLSRNASVEVKNKKGNSPLWLAAHGGHLSVVELLYDHNADIDSQDNRR *F VSCLMAAFRKGHTKIVKWMVQYVSQFPSDQEMIRFIGTISDKELIDKCFDCMKILRSAKE *F AQAVKANKNASILLEELDLERTREESRKAAAARRRERKKKKKMEKKEEKRRQQQGNGPGG *F DDMQGDDDDASDKDDDSDKDDEDEEAAPAAAREEGDSGIDQGSCSSGDTKGARFGGSQSA *F QAAEAAANSVSTNSQGKKNKKQAKNKVLISVEPTQPVITSNSVLKGVCAKKHPAVEVVKQ *F PPATQQAAPLKRQLDVKKEEPALKKKEEKNSSSSSSSKREKENLAPKEVALPAKQQPSSS *F SKLQSSESASNINSSTATNTSSANTTRKEVAKPASQTASATTLNPAKRTEVDGWKEVVRK *F SSAQQTTAVGASGAPLPVTATSSATSVQHHPHHHLANSSSNSSSSLTTSTTTAASSVPEM *F TCKKVQVPVNAISRVIGRGGSNINAIRATTGAHIEVEKQGKNQSERCITIKGLTDATKQA *F HMLILALIKDPDVDILQMLPRINSSIKQASSGGASTPMSVGTWDNRTAAGVNAYTFSSAA *F STTSTSSSSSASSTTPAGASYSNAHKQHQQQPQSVKGPSGRSSTSVKSNGSSTKVSASSG *F SGSRSGRAGSSYLAQQQPGRSSGGGSSNGVIKSKSESSSKSLPAAQKSSTTLGKSSTVSP *F GAQNFAKAAAIGQSSPKKAEGGATSAVVTSAGGRSSGVVAPFGRGKPVAGQGGPAATAAS *F NVAQLGSVSGNSNILAGPIGTFNVADVAAVNAAAAAGAAAATNSNVKPIAPIAPPSKRVG *F SPTQVQQQHQTQQQQQQQLPQPAPVPGPQPQQQPLQQQQQQQAPQQQPQQPNQQQQPQTS *F QQNLVINTNLLNDLMAASAANTTSDSFSAQLAAKLSSAYSLFSDYQQSQWGKLGDPGIGG *F GAGAVGDGLPQADASKAPGYNRNILSSPVGSSKASSNHSTSPPVGNVIQQQQQQQPQSSQ *F QALNIITSGPGGPATAPARSPMVSANEGNPAVGQPSMNGTQGLGETAPAHSPGVIKPPTA *F TVPIQRHVPMPISAPEAGAPPTFGAIGSNPASGNNSAAAQAAAAAAASAMIDRQQQNLQN *F LQTLQNLQRMVGASQQQQPQQQLNYPMDPTSSFIVDANNVLRLNPRVIFPQGNTKPPQPP *F PQGGTQSNVFGGNPGRQPPGTGARQPGGAAAQRWYGGTLEYPSYTGRDMLHLENGAGGMA *F GMGSPSAMSPNHDDIRKMPRPIGTERAASWKYNNFNVGGPSLNMEDALASVLPPWAHELK *F AQPPGLQQPPPPPQSQQQQQQPLNWLKQQPQQQQYRAYNNGPYPQQQQQHEPMNMPMDYH *F NMQAPPNMSQQQQQHVNLMPSYGYQHFVGAPGAVDISAHMPDKMEVWDHHDKHMPWTNYT *F TNWSN *F >AE003746|GENSCAN_predicted_CDS_42|11718_bp *F atgaataatgatgcgaaaaaccatgaaagcgatgacttgaatgtgcgctccacagcgtat *F tttaaccaacaaaccacaaccaatcaaccgaaagcaccagcaaccagcaagaataacaca *F ggctctggctctggatccaataataacaataacaacaccaatcaaaaccccaacagacag *F ttgaatcataatttaccccgaatcgctgccgccagacaatcgatagccgccgctctattg *F aaaaacagcgggcggaagattctgacggccaagaatgagccactgacgacgacggagtca *F tcaggcgttttaaccaacacacctttacccagcaatagccgattgaaagttaacaacaac *F aacaacaccaataacactgccaagatgtctggaactagtagcagtcagtcctcggccacg *F cccacaccgcccacggccagcagcagcacaaccaccacaacaacaacgaacatcagcacc *F ggaggcggtgggagtggcagcagtggcggtggcggtgggagtaccacggtcattgccaat *F cccgcatcggtaaccaacaccggagctggaagtgccgccaagttccgtgccgccgtggcc *F tcggcgcccagcccagcactgcctgcaaccaacgctcctgcaaatgcaactgctgctgcg *F gcaatagcagcaatcgcaactgctcctgcccccagtagtagctcctccagctcctcgtcc *F tcgaagaagactagagcagcagtggccgccctgaagcgacaggtggccttgcaacagcag *F cagcctgttaccggtaatgcacccaacatgaccagcaaggattcggcgcatttgaaattc *F gccacaaccactctgctgatgggcgccgccgcagctgccgccgatagcaacgctggcgct *F gctctcggtggatcaggcgcaggaggatcaggatcatcatcatcagtaggagcggtaggc *F ggggccagaatggccctgaatcccgccgttgatatggccaatgccgctgtcctgcttaag *F caaaagctaaaggatgcggctgccgctgcctcggcctccgcctccaatcgctcagccacc *F tcgtccatgtcgtcaaccgcctcctcgctgtcttcgtcggcgggcatcgtgaatgccatt *F tcctcggcgctgcagaacatcatcacgccggatacggacaccgacaccgaattctatccc *F cagcccgtcaccacagacctatccgaatcggaggaggagtccgtttcagaggacgatatt *F ccagaatcggatccggatagctgtccacacgagggtgaggtgcgcgaggatgaggacgaa *F acggaggaagagtcggaagactctgatgagtctgaaggcgaagaggaagaggaggatgaa *F gaggagatagatgtgctacaggacaacgacgcggacgacgaggagatcgacgatgaggac *F gaggaagaggacgcgcccgaggtgagttcctttctcctggatgccaacaacaagcgttcc *F agcaatatctccgctctgctcgaggccgcggccaatgagaaggctcctgtcctgcgccac *F gccactcacgccatcgacgagaccaagcaggcgctgacaaagatgcgctgcgccagcagt *F ccccgcgataagaacagtggattctccagatccctagtggctgcctgcacggataatgat *F gtcaatacggtgaagcggctgctttgcaagggcaacgtgaacctgaacgacgccgccgcc *F tccacggatgatggcgagtccctgctctcaatggcctgctctgcgggctactacgaattg *F gctcaggttctcctggccatgtctgccgcccaggtggaggacaaagggcaaaaggactca *F acgcctttaatggaagctgcatccgccggtcatttggacatcgtcaaactgctgctcaac *F cacaacgccgatgtgaacgcccactgtgccacgggcaacaccccgctcatgtttgcttgc *F gccggtggtcaggtggacgtggtgaaggtgctgctcaagcacggcgccaacgtcgaggag *F cagaacgagaacggacacactcccttgatggaagcagcttccgccggccacgtggaggta *F gccaaggtgctgcttgaacatggagccggcatcaacacccactcaaatgagttcaaggag *F agcgccctcactctagcctgctacaagggtcacctggatatggtgcgattcctgcttcag *F gcaggtgcagatcaggagcacaaaaccgacgaaatgcacactgccctaatggaggcttca *F atggacggccatgtagaggttgctcgactgctgctggactccggtgcccaggtgaacatg *F cccacggactctttcgagtccccgctaacgttggcggcttgcggtggtcatgtggagttg *F gcaacactcttgatcgagaggggagccaacatcgaggaggtgaacgacgagggctacacc *F ccgctcatggaggccgctcgcgagggacacgaggagatggtagccctcttgctcagcaag *F ggtgcaaacatcaatgccacgaccgaggagacccaggagacagctttgacgctggcctgc *F tgcggtggcttcatggaggtggctgcattcctgatcaaggagggagctaatcttgagctg *F ggtgcttccacgcccctaatggaagcctctcaggagggacacaccgatttggtaagcttc *F ctgctgaagaagaaggcaaatgttcatgcagagacccagacgggggatactgccttgacg *F catgcctgtgagaacggacacacagatgcggccggtgtgctgctatcgtatggagctgaa *F ctagagcacgagtccgagggtgggcgaacgccactaatgaaagcctgtcgtgccggacac *F ctgtgcactgtcaagttcctcattcaaaagggcgctaatgtcaacaaacagaccaccagt *F aatgaccacactgccttgtcgttagcctgtgccggcggtcatcagtctgtggtggagctg *F ctattaaaaaacaacgccgacccgttccacaagctgaaggacaacagcaccatgttaatt *F gaagcctccaagggtggacacactcgtgtggtcgaactgcttttccgctatccgaacatt *F tcgcctacggaaaacgcagcgtctgcgaatgttacccaggcagcaccaaccagcaaccaa *F cctggtccaaatcagatgcgtcaaaagatcatgaagcagcagcttcagcatcagttgcag *F cagctgaacgctccccctggcttgcatgagttgtctgaggcggcacgtgcatccaatcaa *F caacatttccaccagcaacagttcagcagtgccggcaacggatcctccaacatcgtggca *F atgggaactggcgactttttggatgccggagaactccaacttactgctactgcgggaatg *F agtgcgggagccggaaccagtaccacgggcagtgagactggtatggaggagtatggcgaa *F gtcggaggaattgacctgactactcttggcgctcagcagcaagagggtcttattgcaaag *F tcgagattgttccatttgcagcagcagcagcaacaacagcagcagcagcaacaacagcag *F cagcagcagcaacaacaacaacagcagcagcaacaacaacaacagcagccacctgcagct *F ggccagcaccagttagtgccatgtaagcacttcgacctggacatggagcacatcaattct *F ctgcagccgccgcaaaaggcaccgcccgctccacctgtactcttccacaccgtttgccag *F cagcctgtaatgcaacagcagcagcagcagcttcagccaggtcagctcaagttgaaggcc *F atgctacccaaccgcaatcgcgcgttgaaaaccgccgaggtagtggagtttattgactgc *F ccggtggatcaacaacagcctggcgagcaggtgcgcacgcagcctttgggtgaggatgga *F aagactcctcagtttgcatgcgctggagaggatccacggttgcagcgccgtcgcggcttt *F atgccggagctgaagaagggtgaactgccgccggagagcagcagcagtgacccaaacgag *F ctagcccttaaaggttcttattctttgtataattctttacaatcaggagccgacaacaat *F cagcccgtaccgacagcactggacaatagcgcatgcgcccagattccagcgcgaaactct *F ggcggagcaataacccattcctccgaagttctgcagagcacagctatcagcgacaggcca *F aaggtaaaggcaaccaacaagaacaaccgaaagcaagcggccgcagcagcagcagctgca *F gcagcagcggcggcggcagcagcagcggccgcccaacacgcccagcaagtgttgccgaac *F ccaatggtctccatctataataacctgcatttgcagcacttgcagcatccgcatctccag *F tttcagcagcaacttcaactgcatcaccagcgagtagctggactggacaatgcagcggct *F gcagcagcagcagcggcttcatccgcgaacatggcctactctatttctccggcatctcca *F cttccctcgcccactggcagcggcaactatgtcgatcagcagctgcaacagcagtccatg *F gatgttgctctacagcgcaagacggccatggacgatttccgtggcatgttggagacggcg *F gtaaatggtccaaggggcagaaaagacctggctcttaacacaccacagctgaacttcttc *F aaggacggctggcatatggtgggagtgcacaatttctttggtgatcagccaaagtcgccc *F actgaaacaccgcctgaaatggaggagactaccatgtcctcaccgaccgaagcagatcgt *F ctcggatcggagcctcgggccgagatgaagaacttggccacgctctgctcggccgcagca *F gcagctgctgctgtggcagcggttaacaaggatcaggttgagattagttcggatcttgag *F agcgaatgcgaggatgatgcagaaggtggtgctggggcagattgcgaggaaaacacactg *F ccgccggagccaattgaattggcggccgctctaagggaggatggcataattgtggaggaa *F gaagaggatgacgaggaggaggatgatgatgatgaagagcaggataccaacagcggtgag *F gtcgacaagctaaactatgatgacgaagacgcggaggtggacaacgatggtgaagtagac *F tacatcgacgaagacgaaggtggtggagaaggcgaagaagaagaagatgatgcagatgat *F gacgagttcttcttggacgagcctgatagcgaccaaggaactggcaacaataataacaat *F tccaaaagcggcgccagttcgttgccattgaaacagcgcaaaatggccactcggttggaa *F aacctaatcctaaactcgcagacagtgtgcgacttcccgcctgaacttagcaactcggaa *F ctggttcatgtcctgccccaaataagcaatctcaaggcagcggccaacagcaacgcggct *F ctgaacagcgtactccagcagcagttggcagcagcctccgcggcagcggcgcacgccaaa *F gcgtctgtagtccaccagaagcagcagcatggagagggagatcagcaatgcggcgatgtt *F ccgcaggatgcccagcgacaggcgaatcttgtcctcctcgactatcccatgcagcaaaac *F atccaactagagcagcggctactcgatgctgaggaaatgcacctgcagcaacaccagcaa *F acaccgctctccttactgccctttacggatgagcagcagcagcagcttcatcaccaagct *F ttgtccaatgcatccgattttcagcaacaccaacagcttgccctggaaaacgatccagaa *F ctaaagcagcagcttcagcagaactccaacgcgcgcataattaaagctgttgctgcccag *F catcagcagcagcctccaaccaacttcgtttacaacgtggaaagcggcgacaagaatgct *F ccgccagtgcaattgctcttccagttgccaccacacatggcgcaacatcaggcgcagcaa *F cagcagggcgttggagagcctcttaccgaacagcaacagcagcagttacacgctgagcag *F gcgcatctctttcagcatcgaactggcggtcagcgtccgcccacccagagtgagttagag *F caggtggctcaagagctattgcttcagcgaagcggccaggtgccggcaggagctcctgtt *F gttggtgttcaggcaattccactcaagcaaaaacactttaacctgcatccgccgccgtgt *F ccacccacctgtgtccagcatcaggcctctcagcaacagcaaatgcaacaaaacgagctc *F tccatttggccgatggccacgcctactcccgcgcccagcagcggtgtaagctcgaccaag *F tcgatgcccggcggcattgccaaaaaggccattgacaagcagtcgcgcaaggaacgtcgt *F tgcgtggtgcgccagacaccagcaggcattcaagagaacaccaaactccatctacagcct *F caggtcgcaacagcccagcaacaatttctagtgcagaaccagttagcagttgcgaccacc *F gtgagtttggacaagactatcgaaatagattcagagacggaatccaaccacgacacggcg *F ctaaccttggcttgtgccggcggtcatgaagagctggtggaactgttgatcaatagggga *F gcaaacatcgagcaccgtgataagaagggattcacaccgcttatactagccgctaccgct *F ggccacgacaaagtcgtggacattctgctcaagcacagcgctgagttggaggctcaatca *F gagcgtacgaaggatacaccgttgtccctggcatgttctggcggccgatacgaggtggtg *F gaacttctgcttagcgttggtgccaacaaggagcaccgcaatgtatctgattacactcca *F ctgagcttggcagccagtgggggctatgtgaacatcattaaactgttgcttagccatgga *F gcagagatcaattcgcgaacgggcagcaaactgggcatttcaccgcttatgctagccgct *F atgaatggtcatacgccggcggttaagttgcttttagatcagggatcggacataaatgcc *F cagatcgagacgaatcgcaatacggccttgactttggcttgcttccagggcagacacgag *F gtcgtaagtctgctgcttgaccgacgggccaatgtggagcatcgagctaagacgggcctg *F accccactcatggaagccgcgtcaggcggttacatagaggttggtcgcgttctgctggac *F aagggtgcggatgtgaatgctgctccggtgccgacgtccagggatacggctctaacaatt *F gccgccgacaagggccatcaaaagttcgtggagctcctgctatctcgtaatgccagcgta *F gaggtaaaaaataagaagggtaactccccactctggctggctgcccatggtggtcacctg *F agtgtggttgagcttttgtacgaccataatgctgacattgactcacaggataatcgacgt *F gtttcctgtctgatggctgccttccgcaaggggcacaccaagattgtgaagtggatggtg *F cagtatgtgtcccagtttccctctgaccaggagatgattcgcttcattggtaccataagt *F gacaaggagctgatagacaagtgttttgactgcatgaagatcctgcgtagcgccaaagag *F gcccaggccgtcaaggccaataagaatgcttcgatccttttggaggagctggatttggag *F cggactcgcgaagagagccgcaaagctgccgccgcccgtcgtcgtgagcgcaagaagaag *F aagaagatggagaagaaagaggagaagcgccgtcaacaacagggcaatgggcctggcgga *F gacgatatgcaaggcgatgacgatgacgccagtgacaaggatgatgattccgacaaggac *F gatgaagacgaggaggcagcgccggccgccgcccgcgaggagggagactctggcatcgat *F cagggctcgtgctccagcggagacaccaaaggtgcgcgcttcggtggcagtcagtccgct *F caggctgcggaagcggcagccaattccgtgtccaccaacagtcagggaaagaagaacaag *F aagcaggcgaaaaacaaggtgttgatatcggtggaaccaactcaaccagtaatcacatcg *F aactccgtcctcaagggcgtctgtgcgaagaagcatcccgcagtggaagttgtaaagcaa *F cctcctgccacacaacaggctgctcctcttaagcggcaactcgatgtaaagaaggaggaa *F cctgcgctcaaaaagaaggaagagaaaaacagctcgtccagcagcagcagcaagcgtgag *F aaagagaatcttgcgcccaaggaggttgcactgccagccaagcagcagcccagtagctcc *F agcaaactgcagagcagcgagtctgcgagcaacataaacagcagcaccgctaccaacacc *F agtagcgccaatactactcggaaggaagttgcaaagccagcgtcacaaactgcgagtgcc *F accactttgaatcctgcaaagcgcaccgaagtcgatggctggaaggaagtagtccgcaaa *F agtagcgcccagcagaccacagcggtgggagcgagtggagccccactgcctgtcacagcc *F accagttcggccaccagtgtgcaacatcatccgcaccaccacctagccaacagctccagc *F aacagctcaagctccctgaccaccagcactactacagcagcgtcttcggttcccgagatg *F acgtgcaagaaggtgcaagtgcccgtaaatgccatctccagggttattggacgaggtgga *F agcaacattaacgccattcgggccaccactggtgctcacatcgaggtggagaagcagggc *F aagaaccaatcagagcgttgcatcacgatcaagggcttaaccgatgctacaaaacaggca *F catatgctcattttggcactaatcaaggatcccgatgtggacatattgcaaatgctgccc *F aggattaacagcagtattaagcaggcgtctagtggcggagcaagcaccccgatgtccgtg *F ggaacttgggacaatcgcaccgctgccggtgtgaatgcgtataccttttcttctgccgcg *F tccaccacctccacatcctccagctcgtcagctagctctactacaccagcgggagcttcg *F tacagcaatgcgcacaagcagcaccaacagcagccgcagtcagtgaagggcccaagtgga *F cggtcatcaacgtcggtcaagtctaatggcagtagcaccaaggtatcggcttcgagtgga *F tcgggttcccggagcggcagggctggcagtagctatcttgcccaacagcagcctggtcgc *F agctctggaggtggctcttcaaatggcgtgatcaagagcaagtcagaaagctcttccaaa *F tccctgccagctgcacaaaagagcagtaccactttgggtaaatcatcgactgtgtcaccg *F ggtgcacagaatttcgcaaaggcagcggctattggacagtcctcgcccaaaaaggctgag *F ggtggtgctacatctgcggtggtcacctctgccggtggacgcagcagtggcgtggtggct *F ccatttggacgtggcaagcctgtagctggccaaggaggacctgcagcaacggcggcttcc *F aacgttgcccagctgggaagtgtgagtggcaacagcaacatattggctggaccaattggc *F acctttaatgtagcggatgtggctgctgtgaatgcagccgcggcagcaggagcagcagca *F gctaccaacagcaatgtgaaacccattgctcccattgcaccgcccagtaagcgagttgga *F tctcccacccaagtccagcaacagcatcaaacacagcagcagcaacaacagcaactaccc *F cagcccgcaccagttcctggcccacagccacaacaacagccgcttcagcagcagcaacaa *F caacaagctcctcagcagcagccacaacagccaaaccagcaacagcaaccacagacgtcc *F cagcaaaatctcgtgatcaatacaaacctactgaacgatctgatggccgccagtgcagca *F aacaccaccagcgatagcttcagtgcccagctagcagccaagttgtctagcgcatattcc *F ctgttcagtgactaccagcagtcgcagtggggcaagttgggtgatccaggcatcggcggt *F ggagcaggagctgttggcgatggtctgccgcaagcggatgcttccaaggcaccaggatac *F aatcgcaacatccttagctcgcccgttggcagttccaaggcctcgtcgaatcactccacc *F tcgcctcctgtgggtaatgtgatccaacagcagcagcagcagcaaccgcaatctagccaa *F caagctctcaacattatcaccagtggaccaggagggcctgctacagcaccagccagatca *F ccgatggtgtccgccaacgagggcaatcccgctgtgggtcaaccctccatgaatggaacc *F caaggattgggtgagacggctcctgctcattcaccaggcgttatcaaaccgcccacggcc *F acagttcccatccagcgtcatgtgcccatgccgatctctgcgccggaggccggagcaccg *F cccacatttggagcgattggttccaacccagctagcggtaacaattctgcggctgcccaa *F gccgcagctgccgccgccgcctcggcgatgatcgatcgtcagcagcagaatttgcagaat *F ctgcagactttgcaaaatttgcaaaggatggtgggagcctcgcagcagcagcagccacaa *F cagcagctgaactatccaatggaccccacatcgtcgttcatcgtggatgctaataacgta *F ctgcgcctgaatccacgcgtcatctttccgcaaggcaacaccaagccaccgcagccaccg *F ccgcagggtggaacgcagtcgaatgtgtttggaggaaatccaggcagacaaccacctgga *F acgggtgccagacagccaggaggagcagctgcacagcgttggtatggcggcactctggag *F tatccttcatacacgggccgtgatatgctgcacctggagaatggtgccggcggaatggcg *F ggtatgggctcaccatctgccatgtcgcccaaccacgacgacattcgcaagatgccgcgc *F cccataggcactgagcgagccgcatcatggaagtacaacaactttaacgttggaggcccg *F tcacttaacatggaagatgctctagccagtgtgttgcccccatgggcacatgagcttaag *F gctcagcctccaggtttgcagcagccgcctcctccgccgcagtcgcagcagcaacagcaa *F caaccgctcaactggctaaagcagcagccgcagcagcagcagtacagggcctacaacaac *F ggaccctatccgcagcagcagcagcagcatgagccaatgaatatgcccatggactaccac *F aacatgcaggcacctccaaatatgagccagcagcagcagcagcacgtcaacttgatgccc *F tcctacggctaccagcattttgtgggcgctcctggagccgttgacatctccgcacatatg *F ccggacaagatggaggtgtgggatcaccacgataaacacatgccctggaccaactatacc *F accaactggtccaactga *F Column Description *F \------ \------------------------------------------------------------- *F Gn.Ex gene number, exon number (for reference) *F Type Init = Initial exon Intr = Internal exon *F Term = Terminal exon Sngl = Single-exon gene *F Prom = Promoter PlyA = poly-A signal *F S DNA strand (+ = input strand; \- = opposite strand) *F Begin beginning of exon or signal (numbered on input strand) *F End end point of exon or signal (numbered on input strand) *F Len length of exon or signal (bp) *F Fr 'absolute reading frame' relative to start of sequence. *F For example, if nucleotides 1,2,3 of the sequence are read as a codon, that's *F called reading frame 0. If 2,3,4 are read as a codon, that's reading frame 1. *F If 3,4,5 are read as a codon, that's reading frame 2, and so on. *F This information, together with the starting and ending positions of the exon, *F is sufficient to give the amino acid sequence encoded by the exon. *F Another use of the reading frame is that if you see two adjacent predicted *F exons *F separated by a relatively short intron which share the same reading frame, *F it may be worth looking at the possibility that the intervening intron is *F not correct, i.e. that the two exons plus the intervening intron might form one *F long exon (assuming there are no inframe stops in the intron, of course). *F Ph 'net phase' of exon (exon length modulo 3) *F For example, an exon of length 15 bp has net phase 0 since 15 is divisible *F by 3, an exon of length 16 bp has net phase 1 because 16 divided by 3 leaves a *F remainder of 1, an exon of length 17 bp has net phase 2, and an exon of length *F 18 bp has net phase 0 again. The point of this is that exons whose net phase *F is 0 can be omitted from the gene without disrupting the reading frame: such *F exons are candidates for being either 1) incorrect, or 2) alternatively *F spliced. *F I/Ac initiation signal or acceptor splice site score (x 10) *F (If below zero, probably not a real acceptor site.) *F Do/T donor splice site or termination signal score (x 10) *F (If below zero, probably not a real donor site.) *F CodRg coding region score (x 10) *F Low coding region scores may indicate potentially incorrect predictions *F or genes with unusual amino acid and/or codon usage patterns. *F P probability of exon (sum over all parses containing exon) *F This quantity is close to the actual probability that the predicted exon *F is correct. *F Tscr exon score (depends on length, I/Ac, Do/T and CodRg scores) *F An overall measure of exon quality based on local sequence properties # *U FBrf0136733 *a Dobens *b L. *t 2001.6.5 *T personal communication to FlyBase *u *F From leonard.dobens@cbrc2.mgh.harvard.edu Tue Jun 05 14:57:00 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 5 Jun 2001 14:57:00 \+0100 *F Date: 05 Jun 01 09:54:10 \-0400 *F From: Leonard Dobens *F Subject: RE: FlyBase query *F To: Gillian Millburn *F Reply to: RE: FlyBase query *F Dear Gillian, *F You wrote: *F >1. 'B42.1' enhancer trap in Figure 1. Is this enhancer trap a P{lArB} *F insertion ? *F I received this enhancer trap from U. Grossniklaus in 1991. It comes out *F of the Gehring screen. Its full name B42.1M3 (a.k.a. WG1071) and the *F insertion is a third chromosome non-lethal insertion that has FC staining *F indicated in the figure. *F .. *F Best regards, *F Len Dobens *F Gillian Millburn wrote: *F >Dear Dr. Dobens, *F > *F >I am curating your paper for FlyBase: *F > *F >Dobens et al., 2001, Development 128(10): 1845--1856 *F > *F .. *F > *F >1. 'B42.1' enhancer trap in Figure 1. Is this enhancer trap a P{lArB} *F >insertion ? *F > *F .. *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F > *F > *F Leonard L. Dobens, Jr. Ph.D. *F Instructor in Dermatology *F Harvard Medical School *F Research Scientist *F Massachusetts General Hospital *F address: *F Cutaneous Biology Research Center *F Building 149, 13th Street *F Charlestown, MA 02129 *F (617) 726-4454 *F FAX: (617) 726-4454 *F after September 1, 2001: *F School of Biological Sciences *F University of Missouri-Kansas City *F 103 Biological Sciences Building *F 5007 Rockhill Road *F Kansas City, Missouri 64110 # *U FBrf0136734 *a Goldstein *b E.S. *t 2001.6.5 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Jun 05 17:30:06 2001 *F To: e.goldstein@asu.edu *F Subject: FlyBase query *F Dear Dr. Goldstein, *F I am curating a paper for FlyBase: *F Park et al., 2001, Development 128(10): 1899--1909 *F in which they use some eve alleles provided by you. *F One of the alleles is called 'eve14-10' ( = superscript). *F I am writing to ask whether this is the same mutation as the one called *F '14-10' (in complementation group F) in: *F Bour et al., 1995, Genes Dev. 9(6): 730--741 *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From e.goldstein@asu.edu Tue Jun 05 17:53:40 2001 *F Subject: Re: FlyBase query *F To: 'Gillian Millburn (Genetics)' *F yes it is, if there is anything else, let me know. *F elliott goldstein *F .. *F Elliott S. Goldstein *F Biology Department *F Molecular and Cellular Biology Program *F Arizona State University *F Tempe, AZ 85287-1501 *F 480-965-7176 *F e.goldstein@asu.edu # *U FBrf0136753 *a Donaldson *b M. *t 2001.6.18 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Thu Jun 14 17:04:17 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 14 Jun 2001 17:04:17 \+0100 *F To: dmg25@mole.bio.cam.ac.uk *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Thu, 14 Jun 2001 17:04:19 \+0100 *F Content-Length: 728 *F Dear David, *F I am curating your paper: *F Donaldson et al., 2001, J. Cell Biol. 153(4): 663--676 *F Could you tell me the line numbers for the two polo alleles 9 and 10 *F which are from the Deak collection, e.g. 1460/6 so that I can rename *F the relevant alleles in FlyBase and avoid duplicating information in *F the database, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From md260@mole.bio.cam.ac.uk Mon Jun 18 11:12:09 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 18 Jun 2001 11:12:09 \+0100 *F Date: Mon, 18 Jun 2001 11:08:40 \+0100 *F From: Mary Donaldson *F Organization: CRC Cell Cycle Genetics Group *F X-Mailer: Mozilla 4.7 en-gb (WinNT; U) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: Gillian Milburn *F Subject: Alleles *F Content-Transfer-Encoding: 7bit *F Hi Gillian, *F The polo 9 and 10 alleles have the line numbers 256/4 (9) and 1324/8 *F (10). *F Mary *F \-- *F Mary Donaldson *F PhD Student *F CRC Cell Cycle Genetics Group *F Department of Genetics, *F University of Cambridge. *F Downing Site, *F Tennis Court Road, *F Cambridge. *F CB2 3EH *F Telephone: *F Lab: 01223 766 595 *F Home: 01223 762 484 *F Mobile: 07970 741 250 # *U FBrf0136759 *a Pelisson *b A. *t 2001.6.21 *T personal communication to FlyBase *u *F From gm119@gen.cam.ac.uk Tue Jun 19 15:59:26 2001 *F To: alain.pelisson@igh.cnrs.fr *F Subject: FlyBase query *F Dear Dr. Pelisson, *F I am curating your paper for FlyBase: *F Robert et al., 2001, Genetics 158(2): 701--713 *F 1. There are a number of EST clots drawn in Figure 4. *F They are: *F clot#312 *F clot#1926 *F clot#10043 *F clot#6898 *F clot#9754 *F for each clot, could you give me the symbol of an EST that is in the *F clot (if possible all the ESTs from each clot). e.g. LD12345 so that I *F can unambiguously identify which ESTs are in the clot \- the clot numbers *F on the BDGP site are not stable and the ESTs within each clot change *F with time so I cannot be sure that if I look up the clot numbers on the *F BDGP page whether the ESTs that are in the clot now are the same as *F when you looked at the clot pages. *F 2. Does clot \#9754 in Figure 4. correspond to 'TO42' in GenBank *F accession AF182444 ? *F I look forward to hearing from you, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Alain.Pelisson@igh.cnrs.fr Thu Jun 21 07:40:04 2001 *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query *F Dear Dr. Millburn, *F ... *F clot#312 is represented by GH04962 (which matches the putative gene *F 'CG14476') *F clot#6898 is represented by HL02945 *F clot#9754 is represented by two overlapping ESTs: GH24711 and GH08843. By *F the way, concerning your second question , TO42 (AF213379) does indeed *F match the ESTs of clot#9754, except for the 3' end , because of an *F alternative polyadenylation. *F You also ask for the names of ESTs expressed from the distal part of the *F D1/D1' duplication (referred to as clots#1926&10043 in Fig.4): in fact, *F as written at the bottom of page 707, these ESTs appeared to correspond to *F alternative splicing variants (represented by GH25094 andLD08455) which *F might originate from either (or both) of the duplicated transcription *F units. However, since only the distal copy of the duplication was *F sequenced, we could not address this question any further as we *F tentatively did for the pair of clots \#6898☚ (see top of page 708). *F ... *F Hoping all is now as clear as possible, I thank you for curating these *F data. *F Alain *F \* PELISSON Alain *F \* I.G.H. *F \* 141, rue de la Cardonille *F \* 34396 MONTPELLIER Cedex 5 (FRANCE) *F \* Tel. :+33(0)4 9961 9944 (Dial (0) only within France) *F \* Fax : \+33(0)4 9961 9901 *F \* e-mail : Alain.Pelisson@igh.cnrs.fr # *U FBrf0136760 *a Whitfield *b E. *t 2001.6.25 *T personal communication to FlyBase *u *F Date: Mon, 25 Jun 2001 10:15:35 \+0100 *F From: Eleanor Whitfield *F To: flybase-updates@morgan.harvard.edu *F Subject: CG1786 is Cyp318a1 *F Hi, *F Currently in the genes file CG1786 exists as its own gene. *F David Nelsons (P450 expert at the jamboree) web site *F (http://drnelson.utmem.edu/droso.all.P450s.html) shows that CG1786 is *F the translation for Cyp318a1. *F Could you please merge these genes. *F thanks *F Nellie # *U FBrf0136763 *a Haecker *b U. *t 2001.5.30 *T personal communication to FlyBase *u *F Date: Wed, 30 May 2001 09:01:49 \-0500 (EST) *F From: fbmailer@bio.indiana.edu *F Reply-to: udo.haecker@medkem.lu.se *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase reports on frc/CG3874 *F comments: It has come to my attention that there are separate flybase reports *F for the genes frc (fringe connection) and CG3874. frc and CG3874 are synonyms *F for the same gene. A functional characterisation of frc/CG3874 by Selva et al. *F is in press in Nat. Cell Biol. *F mailto: flybase-help@morgan.harvard.edu *F realname: Udo Haecker *F reply-to: udo.haecker@medkem.lu.se # *U FBrf0136764 *a Whitfield *b E. *t 2001.5.25 *T personal communication to FlyBase *u FlyBase error report for CG18838 on Fri May 25 07:06:59 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 25 May 2001 07:06:59 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG18838 on Fri May 25 07:06:59 2001 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG18838 *F Release: 2 *F Gene annotation error *F Gene CG18838 has incorrect exon/intron structure or translation start site. *F Comments: I wonder if this translation should be extended at the C-terminus? *F The current translation has a match for PROSITE pattern PS00134 at the very *F C-terminus. PS00134 is a pattern that picks up the N-terminus of the trypsin *F motif so I wonder if the other domains that define a Trypsin family: *F PROSITE pattern:PS00135 *F PROSITE PREFILE:PS50240 *F PFAM:PF00089 *F SMART:SM00020 *F (taken from InterPro IPR001254) *F would be found in further 3' exons? *F Is this a member of the Trypsin family or is the pattern match to PS00134 bogus? *F thanks *F Browser: Mozilla/4.75 en (Win95; U) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0136765 *a Wilkie *b G. *t 2001.5.25 *T personal communication to FlyBase *u FlyBase error report for CG1664 on Fri May 25 14:36:30 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 25 May 2001 14:36:30 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: Gavin.Wilkie@hgu.mrc.ac.uk *F Subject: FlyBase error report for CG1664 on Fri May 25 14:36:30 2001 *F Error report from Gavin Wilkie (Gavin.Wilkie@hgu.mrc.ac.uk) *F Gene or accession: CG1664 *F Release: 1 *F cDNA or EST error *F Gene cDNA sequence: *F >sbr|cDNA sequence *F ATCAAGCGAATATACTTCGCTCGCTACATATTTCTAGTTAGTTTTCGTAACTTTTTCGCTTTGGTGGCAATTAAATCAAA *F TTTCCTCGAAGAATAACTTGTAAAAAGGTCGCAAACATCCGTCCAGTTTCTGCCAACCATCGGCCACTGGCTATCGAAAG *F AAGATCGCTAGTGAATTAAATTTCACAAAATTGCGTTGTACTTTGGCCGTGGAAATTTGCCAAAAAACAGCAGCTACTTT *F GCTGCTATATTAGCAAATAGGTTCAGGAAGTCGAGAAAAATTTTTCCAGAAGTTGGCAGCAGTTTGTGTGAATGCAAAAA *F ATACATCGCACATAGTTTTTTGCCCTCAAGACAGTTTTCTCAGTAATAACAACAGCACCCACACAAACACACAACACCAC *F TCAGCTAAAAACGTGCTGAAATTCGCATATCTTTTTGTTGCATACGTTTTTGAGTGAATATTAGTAAGATGCCCAAACGC *F GGCGGTGGCAGTAGCCAGCGGTACAACAACAACGTTGGAAATGGCGGCGGACGTTACAACGCTCCCGAGGATTTCGATGA *F TTTTGATGTGGAGGATCGCCAGCGACGCAAGGATCGAAACAAGCGGCGCGTCAGCTTTAAGCCCTCCCAATGTCTACATA *F ACAAAAAGGACATCAAGCTGCGACCCGAAGATTTGCGTCGATGGGACGAGGATGATGACATGAGCGACATGACCACGGCC *F GTTAAGGATAGACCCACCTCCCGACGTCGGGGATCGCCCATTCCGCGCGGCAAGTTCGGCAAACTGATGCCCAACAGCTT *F TGGCTGGTACCAAGTCACGTTACAAAACGCCCAGATATACGAAAAGGAAACACTCTTGAGTGCTCTATTGGCAGCGATGT *F CGCCACATGTCTTTATTCCTCAATATTGGCGAGTGGAGCGAAACTGCGTAATCTTCTTTACGGACGACTACGAGGCAGCC *F GAACGCATTCAACATCTGGGCAAGAATGGCCATCTTCCAGATGGCTATCGTCTGATGCCACGAGTACGCAGCGGTATACC *F ACTAGTGGCCATCGACGATGCCTTCAAGGAGAAGATGAAGGTCACAATGGCCAAGCGTTACAATATTCAAACCAAGGCGC *F TGGATCTTTCCCGTTTTCATGCAGATCCGGATCTTAAGCAAGTTTTCTGCCCACTCTTTCGTCAGAATGTGATGGGCGCT *F GCCATTGACATTATGTGCGACAATATACCCGATTTGGAGGCACTTAACCTGAATGACAACTCCATTAGCAGCATGGAGGC *F GTTTAAGGGTGTGGAGAAACGCTTACCGAACCTCAAGATTCTCTATTTGGGGGATAACAAGATACCATCTTTGGCCCACC *F TTGTAGTGCTTCGCAATCTGTCCATCTTGGAACTCGTTTTAAAGAACAATCCCTGCCGTTCCCGCTACAAGGATTCCCAG *F CAGTTTATCAGCGAAGTACGTCGCAAGTTTCCCAAACTGGTTAAGTTGGACGGAGAGACCCTGGAGCCGCAAATCACATT *F TGATCTATCCGAGCAGGGACGTCTTCTCGAAACGAAGGCATCCTATCTGTGCGACGTCGCTGGTGCCGAGGTGGTGCGCC *F AGTTCCTGGACCAGTACTTCCGCATATTTGACTCGGGCAATCGGCAAGCTCTGCTAGATGCCTACCATGAGAAAGCGATG *F CTCTCCATATCAATGCCTTCGGCCAGTCAGGCGGGCAGATTGAACAGTTTCTGGAAGTTCAATCGCAATCTCCGGCGCTT *F GTTAAACGGCGAAGAGAATCGCACCCGAAACTTGAAGTACGGACGCCTGGCATGTGTTTCCACATTGGATGAATGGCCAA *F AAACGCAGCACGACCGACGCACCTTCACCGTCGACCTGACCATCTACAATACTTCAATGATGGTTTTCACCGTGACGGGA *F TTATTCAAAGAGCTGAACGACGAGACCAACAATCCCGCCTCCATGGAATTATATGACGTTCGCCACTTTGCCCGCACCTA *F CGTGGTGGTGCCACAGAATAATGGCTTTTGTATCCGCAACGAGACGATCTTCATCACAAACGCTACGCACGAGCAGGTGC *F GAGAGTTCAAGCGATCGCAGCACCAGCCTGCTCCCGGAGCTATGCCCTCCACTTCCAGTGCAGTGACCAGTCCTCAGGCC *F GGGGCAGCGGCGGGTCTGCAGGGTCGTCTGAATGCGTTGGGCGTGGCCACTGGACCGGTGGCTATACTATCAGGAGATCC *F GTTGGCGGCCACCGCACCGGTTAACAGCGGCAGTGCCGCCATATCGACAACAGCAGTGGCACCTGGCGCCCAGGATGAGA *F GCACTAAAATGCAAATGATTGAAGCCATGAGCGCCCAAAGCCAAATGAATGTGATCTGGAGTCGGAAATGCCTGGAGGAA *F ACGAATTGGGACTTTAACCATGCCGCCTTTGTGTTCGAGAAACTATTCAAGGAAAACAAAATACCGCCTGAGGCTTTTAT *F GAAGTAAATCGCATAGGAGTTTCCGTAGGACAGAGCCGCGTGCCACATCCACATAATCGAATGCTGTTTTTTTTTTTTTG *F GTTTTGTAATTAAATTTTAAAATTATTAGAGAAACCTCTATATAATAATAATAATTAATATTATTAAGCTGCGAAGTTGT *F GTGCACATTCGGGCAGTAGCAATTATTATCCCAGCACTGCGGGCAATGTGCATCAACGATCACAGTTCTTCGATAGATTA *F GTTTAGCTCTCTTTAAGTTCCGTCCGCAGATCCGCTGGTCTACATTGAGGCCAGGACGAGTCTGCGAACGGTAGTCCCTT *F TAGAGTTAAAGTTGTTTTAGATTCCTAAGCCAAACACCTTCAAACACACACAACACGACAACACTATTAAACGTAATGCA *F ACAATGTTGTCGAATCGAAAGAAACCCAATTTTTATTTTAATATATACGACGATACCGAAACCAAGGCGATGGTCGAAAA *F GCATGGATCGCGCCAATAATTCTATATTCCCCGCTTTCCCAGATCCTCGACTCGCCTTACATTTTGTATACAAATACCAT *F AGAATAAAAAGAAACATTTTGACCACTGTAAAAATTTTGTAACGACTCGGAAACCAAAATTACCTTTATTTCTTAATAGA *F AAAAAATTATTCGATTTACAATACACGCTTAGCCGTAAATTCAATTGAATTTAAGTGTAAGATATATAAAAATTATATAC *F ATTAAGACAAAAGTGTAGATTCATAAATAAATTTTTCAGAATAGAAAAAAAAAAAAAAAAAAAA *F Protein sequence: *F >Sbr|Protein Sequence|mRNA export protein *F MPKRGGGSSQRYNNNVGNGGGRYNAPEDFDDFDVEDRQRRKDRNKRRVSFKPSQCLHNKKDIKLRPEDLRRWDEDDDMSD *F MTTAVKDRPTSRRRGSPIPRGKFGKLMPNSFGWYQVTLQNAQIYEKETLLSALLAAMSPHVFIPQYWRVERNCVIFFTDD *F YEAAERIQHLGKNGHLPDGYRLMPRVRSGIPLVAIDDAFKEKMKVTMAKRYNIQTKALDLSRFHADPDLKQVFCPLFRQN *F VMGAAIDIMCDNIPDLEALNLNDNSISSMEAFKGVEKRLPNLKILYLGDNKIPSLAHLVVLRNLSILELVLKNNPCRSRY *F KDSQQFISEVRRKFPKLVKLDGETLEPQITFDLSEQGRLLETKASYLCDVAGAEVVRQFLDQYFRIFDSGNRQALLDAYH *F EKAMLSISMPSASQAGRLNSFWKFNRNLRRLLNGEENRTRNLKYGRLACVSTLDEWPKTQHDRRTFTVDLTIYNTSMMVF *F TVTGLFKELNDETNNPASMELYDVRHFARTYVVVPQNNGFCIRNETIFITNATHEQVREFKRSQHQPAPGAMPSTSSAVT *F SPQAGAAAGLQGRLNALGVATGPVAILSGDPLAATAPVNSGSAAISTTAVAPGAQDESTKMQMIEAMSAQSQMNVIWSRK *F CLEETNWDFNHAAFVFEKLFKENKIPPEAFMK *F Comments: Complete cDNA sequence for Sbr gene, which encodes the Drosophila *F homologue of human TAP/NXF1 and yeast Mex67p. The length of this cDNA sequence *F matches the size of the transcript detected by Northern analysis. # *U FBrf0136766 *a Roos *b C. *t 2001.6.14 *T personal communication to FlyBase *u FlyBase error report for CG13780 on Thu Jun 14 23:25:17 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 14 Jun 2001 23:25:17 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: christophe.roos@helsinki.fi *F Subject: FlyBase error report for CG13780 on Thu Jun 14 23:25:17 2001 *F Error report from Christophe Roos (christophe.roos@helsinki.fi) *F Gene or accession: CG13780 *F Release: 2 *F cDNA or EST error *F Gene cDNA sequence: *F >CG13780_CDS *F ATGCAATATTTAAATATGAATCTCGTAATATGCAATCTCGTGCTGGCGTTCATCGTCATT *F ACATTTTCGAAGACGACACACGCTTATTCCAGTTTTCCAACTGACATAAATAGCAATTAC *F TTCGACGTGCGGAATTCGACCTTGCCCATGACCATGACCACCTCCCATCCACAAACCAAT *F CGTCCTCTCGCTCGCTACGTTCTCCCCCCAAACGAGTTGCATACCGCGGACGAGGACGAA *F GAGTCCTCTGACTTAGAGCCGTACTTTGAGGATCGAGCGGTCCAGCGGGACAGGTCGTAT *F CTCATCCGATTGATCCGCCGGCGCGTGCCACGTGATCAGGCCCCCCAGCAAGTAATTCTT *F CAGGGCCGCAGTCTCAGTGGCGTTCACTGGGGGCAGGGCGACGACAATCATCTCAGCTCC *F GATGCGGCCGTCGATGGGAGCGATGACTATCCATCGGATCAACCGGGCGATTTGACAGTG *F TTGAAAGAGCGAATCGCCGAACAAAACTCCGTTGAAAAGTTGAGGACTATGAAATACAAC *F AATAATCGTACCAGGGAGCTCAAAAAAAGGGTCGAGGCACACCGACTGATGATGGCAAAG *F GAGGGTATCTGCCGGGTTCCAAGACCGGAGGTGGTCCACATCACGAGAGAAACGAACACG *F TTCTATTCGCCCCGTGCCACAATTCTGCACAGATGCAGCGACAAAGTCGGATGCTGCAAT *F GCAGGTTGGACCTGCCAGATGAAGAGGAACGAAACGGTGGATCGGGTGTTCGACAAAGTG *F GATGGCCGTTCCAATGAGCCCATCGTTATCTCGATGGAGAACCACACGGAATGCGGATGT *F GTGAAGGTGGAAACGCGCCGGAAACGAAGTCCCATTTGCCTATGCCCCAAGCACTTCAAG *F GACTTCAGTTGGGCGGGATCGCGTGCGCAGTGGGAGAATGAGGAGCACCTGGAGCTGCGT *F CTGTGGGAGCGGAGGGAGCAGCGCTGCCGATGCGACTGCCACCTCAGCGACGAAACGTGC *F AAGAGGCTGAAAAACGGCGTTGAGGGATTCTCGGTGATGGAGCGACGACGCATTCAAAGT *F GGAGAAGTCAGTCCGCCGTTTTGCAACTACGGGGCATATGATGTGAGGAACGGTCGATGT *F CCACGCCCGGGTCTTCCAAATCGGAATCCCAATCTGCAACAGCGTTTGCAGTCAAAACGT *F CAGAATGGCAAAAGCTAA *F Protein sequence: *F >CG13780_pep *F MQYLNMNLVICNLVLAFIVITFSKTTHAYSSFPTDINSNYFDVRNSTLPMTMTTSHPQTN *F RPLARYVLPPNELHTADEDEESSDLEPYFEDRAVQRDRSYLIRLIRRRVPRDQAPQQVIL *F QGRSLSGVHWGQGDDNHLSSDAAVDGSDDYPSDQPGDLTVLKERIAEQNSVEKLRTMKYN *F NNRTRELKKRVEAHRLMMAKEGICRVPRPEVVHITRETNTFYSPRATILHRCSDKVGCCN *F AGWTCQMKRNETVDRVFDKVDGRSNEPIVISMENHTECGCVKVETRRKRSPICLCPKHFK *F DFSWAGSRAQWENEEHLELRLWERREQRCRCDCHLSDETCKRLKNGVEGFSVMERRRIQS *F GEVSPPFCNYGAYDVRNGRCPRPGLPNRNPNLQQRLQSKRQNGKS *F Comments: CDS from cDNA *F Embryonic 0-22h library # *U FBrf0136767 *a Beckendorf *b S. *t 2001.6.22 *T personal communication to FlyBase *u FlyBase error report for CG15455 on Thu Jun 21 16:57:16 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 21 Jun 2001 16:57:16 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: beckendo@uclink4.berkeley.edu *F Subject: FlyBase error report for CG15455 on Thu Jun 21 16:57:16 2001 *F Error report from Steven Beckendorf (beckendo@uclink4.berkeley.edu) *F Gene or accession: CG15455 *F Release: 1 *F Gene annotation error *F Gene CG15455 has a mistake in the supporting evidence or functional assignment. *F Comments: From 'Blast 2 Sequences' results without filters CG15455 is similar *F to CG5111 from 2-569 of CG15455 and from 50-613 of CG5111. CG15455 therefore *F does not have runt domains or an AML1 domain. Instead it has 2 (or 3) UBA *F domains. It is not likely to be a transcription factor and there is little *F justification for it being nuclear. The most similar protein in nr, as for *F CG511, is Nub1. # *U FBrf0136777 *a Swan *b A. *t 2001.6.4 *T personal communication to FlyBase *u *F From aswan@molbio.princeton.edu Mon Jun 04 13:19:53 2001 *F Envelope-to: hb246@gen.cam.ac.uk *F Delivery-date: Mon, 4 Jun 2001 13:19:53 \+0100 *F From: 'Swan, Andrew' *F To: 'Heather Butler ' *F Subject: RE: *F Date: Mon, 4 Jun 2001 08:15:17 \-0400 *F MIME-Version: 1.0 *F X-Mailer: Internet Mail Service (5.5.2653.19) *F ... *F the paper is Genome Research/11/67-77 *F the mistake is the map position of fs(1)124 should be 9D1-9F10. *F .... # *U FBrf0136779 *a Kasuya *b J. *c L. *d Iverson *t 2000.11.13 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu May 24 21:19:19 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: DsRed Insertions *F Personal communication from: Junko Kasuya and Linda Iverson, Beckman Research *F Institute of the City of Hope *F To: Bloomington Drosophila Stock Center *F Subject: UAS-DsRed Insertions *F Dated: 13 November 2000 *F Background: Stocks with these insertions were donated to the Bloomington *F Stock Center. Mapping of the insertions to chromosome was done at the stock *F center. Stocks to be added to the Bloomington collection contain the *F following insertions: *F Insertion Chromosome Phenotype of chromosome with insertion *F P{UAS-AUG-DsRed}A 3 viable, fertile *F P{UAS-AUG-DsRed}B 2 viable, homozygotes essentially sterile *F P{UAS-AUG-DsRed}Ea 1 viable *F P{UAS-AUG-DsRed}Eb 2 uncertain *F P{UAS-AUG-DsRed}Ec 3 uncertain *F Technical information on DsRed (Discosoma Red fluorescent protein) is *F available at *F http://www.clontech.com/archive/OCT99UPD/RFP.html *F Information communicated: *F 'We generated several independent lines of Clontech's DsRed in the pUAST *F vector and tested the flies using various Gal4 drivers to make certain that *F they express DsRed in the correct tissue-specific pattern. We also tested with *F GFP driven expression and there does not seem to be any problem detecting both *F GFP and DsRED in the same fly.' # *U FBrf0136780 *a Umea Stock Center *b ?. *t 2001.5.25 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri May 25 16:25:47 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(1)RR79 *F The following information accompanied stocks from the Umea Stock Center *F (4/01). *F Df(1)RR79 has breakpoints 16C;16F. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136781 *a Artero *b R.D. *c M.K. *d Baylies *t 2001.5.25 *T personal communication to FlyBase *u *F From r-artero@mskmail.mskcc.org Fri May 25 22:21:04 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: etymology for gene 'hibris' *F Dear sirs, *F We would like to update the hibris entry to Flybase with the gene's etymology. *F hibris mutant embryos and overexpression of Hibris leads to partial *F myoblast fusion block. We envision these free myoblasts representing *F rebelliousness, insolence, and transgression of rules in the *F mesodermal 'society', the three qualities that the minor Greek *F Goddess 'hibris' represents. *F Sincerely yours, *F Ruben D. Artero *F Mary K. Baylies *F \-- *F Ruben D. Artero E-mail: r-artero@ski.mskcc.org *F Memorial Sloan-Kettering Cancer Center *F 1275 York Avenue. Box 310 Lab phone number: (212) 639 5953 *F New York, NY 10021 FAX: (212) 717 3623 # *U FBrf0136782 *a Hay *b B. *t 2001.5.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 29 16:30:21 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-p35.H}BH3 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Bruce Hay, Caltech (4/01). *F P{UAS-p35.H}BH3 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136783 *a Bonini *b N. *t 2001 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 29 16:43:48 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-ey.H}UE11 insertion *F The following information accompanied a stock donated to the Stock Center *F by Nancy Bonini, University of Pennsylvania (5/01). *F P{UAS-ey.H}UE11 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136784 *a Umea Stock Center *b ?. *t 2001.5.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed May 30 15:04:19 2001 *F To: Rachel Drysdale (Genetics) *F Subject: CyO variant *F The following information accompanied a stock donated to the Bloomington Stock *F Center by the Umea Stock Center (4/01). *F An apparently unpublished CyO balancer chromosome carries an insertion of *F P{ry+t7.2=ftz/lacC}. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0136785 *a Bayraktaroglu *b L. *t 2001.6.13 *T personal communication to FlyBase *u *F From leyla@morgan.harvard.edu Wed Jun 13 19:47:21 2001 *F Subject: P{EP}EP356 can be curated as an allele of svr *F To: cambridge-curators@morgan.harvard.edu *F Dear Cambridge curators, *F By alignment, the insertion site for P{EP}EP356 falls within the *F 5' UTR of svr. I used ESTs to extend one of the svr transcripts (added *F a non-coding exon to the 5' end); P{EP}EP356 aligns to the 'new' exon, *F and the insertion site falls within this exon. Let me know if you *F need more information. *F Thanks very much, *F Leyla # *U FBrf0137134 *a Lankenau *b D. *t 2000.2.28 *T personal communication to FlyBase *u *F Archived: map of micropia retrotransposon *F *F Updated version of map of micropia originally published in FBrf0078865. # *U FBrf0137135 *a Wakabayashi-Ito *b N. *t 2001.7.19 *T personal communication to FlyBase *u *F The following information was communicated by Dr. Noriko Wakabayashi-Ito *F on Mon, 9 Jul 2001 to Leyla Bayraktaroglu. *F *F Sequence of the first exon of the unpublished fusilli cDNA: *F *F AGCGGATGCGCGACGGGTTAAATAAGTTAATAAACAGATTCGACACACACACACACCGAAACGTTT *F GCACGCACACTCATACCAATTCAACAACTAGAAAAAGACAACAAGACAAAGTGGTGGAAGCAACAG *F GTCAAAAAGAAGAAGAAAATACTTGTTTGCTGGCTTTAAATTAAAATAGTAAACTATTCAAAAGGC *F CACAAAACAAAAATAGCAGGCGTTTTATCAAATGTAACTGTAACACATATGGTCTTTTCGAGTTTG *F TGTGCGAGAGAAAAGAAAGTGCAATAAAAACCAAAATTTGTGAAAAGCCAACAAGTGCAATGTATA *F CAACGTGTTGAACAGAAAAAAAATCATAAAATAAGAAAACAGTAAAAACAGTTAAGTCGCGTCGCT *F GAGAGAGGGAGAAAAAAG *F *F [Curator's note: this is first exon of the alternately spliced 5.2 kb cDNA mentioned in *F FBrf0132288.] *F *F Sequence of the transcript which mapped to upstream of fusilli transcript. It expands *F from 1 to 1060 and 1209 to 1654 ( there is a small intron 1060-1208 ) in AC008369. *F I think it is the same gene called CG8207: *F *F The sequence is : *F GGCACGAGGTATTTTCTAGCTGGCAACGTAAACATAAAATATATCTTTCTGAGAACCTGGCCACAAGTTGATCG *F ATCGTCATTAGTCATCTTACCGCTGCACGCACACCATGCTAAAGGCCGTTATCCTGATCGGCGGTCCCCAGAAGGGCACC *F CGCTTTCGCCCCCTTTCGCTTGACACGCCCAAGCCGCTCTTTCCATTGGCCGGTCGTCCTCTAATCGCCCATCATATTGA *F GGCCTGCGCCCAGCTGCCCGATATACGCGAGATCCTGATCATCGGCTATTATCCGCAGACCCAAATGGAGGGATTCGTGG *F GCGACATGCAGGCCCTGTACAGCAGCAGCAACATCAACATCAGGTACCTTCAGGAGTTCACTGCCTTGGGCACTGCAGGC *F GGAATGTACCATTTCCGGGATCAGATTAGAGCTGGTAACCCCCGAGCCTTCTTCGTATTGAACGGAGATGTTTGCGCTGA *F TTTTCCGCTGCAAGAACTGTGCGACTTTCACGAGAAGCGCCCGGCGAGTGCTCTGGTTACTATCATGTCCACGGAGGCGA *F CACGTCAGCAATCCTTGCACTACGGCTGTCTGGTTTTCGATCGGAGTAGCGGAGCCGTTTCGCATTATGTAGAAAAGCCT *F AGCTCCTATGTGTCCACATTTATCAACTGTGGGGTGTATGTCTGTTCCATGGATATATTCACGGTGTTGGCACAGATTTT *F TCATTCGAGAGGTCAGGAGTACAGCTGCCAGGCATTTTGCAATGGAAACGGAAATGGAAATGGACGAGAGCAGGGGCACA *F TCAAGTGGGAACAGGAGGTGCTTAACGCCCCTGGCTGGAACCGATAAACTCTTTGCCATGCCAGTGCCTAACTGGTGGTC *F CCAACTGAAGACTGCTGGGTCGGCAATCTACGCAAATCGTCATTATCTTGGCCTGTACAAGAAAACGCATCCCGAGAGAT *F TGGCCAATGTGGGGATCAAGCGCGGAGAAGGGGACGGCAGCTTGATCTGCACAGTGCATCCGGACGTCTATGTGCATCCC *F AGTGCCACTGTCCATCACAGCGCAGTGCTGGGACCCAATGTGGCGATTGGACCTGGAGTGACAATTGGACCAGGTGTGCG *F CATTCGCGAATCGATTGTTTTGGAGCAGGCACAGATCCTGGACCATACGCTCGTGCTGCACTCTATTGTGGGCAGGGGCT *F CCACCATTGGAGCTTGGGCCTCGAGTCGAGGGCACACCTAGTGATCCCGATCCCAACAAACCCTTTGCCAAAATGGAGAA *F CCCACCGCTTTTCAACAACGAGGGCAAGCTAAACCCCTCGATAACCATACTGGGCTGTTTCGTGCAGGTGCCCGCCGAGA *F AAATCCTGCTGAACAGCATTGTACTGCCGCACAAGGAGCTCAGTCGCAGCTTCAAAAACGAGATCATCTTGTGAGAATGA *F TTTTATTTTTTATCTTCGCTCTGTGTCCCTATCAGTATGTCATTTTATATGTTTATATTGTGCTATATTCTGAAAAACTC *F CCTATTAAATGTGTCTTATCCTCTGCAGCCAAAAAAAAAAAAAAA *F *F [Curator's note: BLAST at BDGP indicates that this does correspond to CG8207] # *U FBrf0137420 *a Burbelo *b ?. *t 2001.7.27 *T personal communication to FlyBase *u *F Date: Fri, 27 Jul 2001 16:03:27 \-0400 *F From: Peter Burbelo *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (cy1236) *F Chiro, *F Here they are! *F All the best. *F Peter *F Name molecule *F CRIB-sequence Accession *F dPAK1 S/T kinase NISYP-TNFEHTVHVGF AAF54131 *F (AE003675) *F dPAK2 S/T kinase LISMP-SNFEHRVHTGF CAA09699 *F (AE003502) *F dPAK3 S/T kinase EIGAP-TNFQRHFHVSR AAF55316 *F (AE003713) *F dMLK2 S/T kinase QISLP-TGFRHTITAVR AAF46344 *F (AE003443) *F dMRCK S/T kinase MISAP-TNFNHISHMGP AAB96643 *F (AF029395) *F dACK Y kinase MISKPQNDFKHTGHVGD AAF58423 *F (AE003819) *F dWASP ABP DISRP-TNFVHLSHVGW AAF56819 *F (AE003767) *F dSPEC2 Adapter MIGNP-TNFVHTGHIGS AAF51990 *F (AE003602) *F dPAR-6 Adapter SISIP-HDFRQVSAIID AAF48757 *F (AE003506) *F Chihiro Yamada wrote: *F > Dear Dr Burbelo, *F > *F > I am currently curating your paper for FlyBase: *F > *F > Pirone et al., 2001, Trends Genet. 17(7): 370--373 *F > *F > I have a few quick questions for you, *F > *F > In your review in Fig 1. you mention four Drosphila genes below: *F > *F > dMRC *F > dPAK2 *F > dPAK3 *F > dSPEC *F > *F > We do not have genes in FlyBase. Presumably they each correspond to a *F > CG resulting from the Drosophila genome project. Can you tell me which *F > CG corresponds to which gene? *F > *F > Thanks and best wishes, *F > *F > Chihiro *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: c.yamada@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \---------------------------------------------------------------------- # *U FBrf0137428 *a Scott *b K. *c R. *d Axel *t 2001.4.25 *T personal communication to FlyBase *u *F [FlyBase curator comment: data taken from web page *F http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html on 25.4.2001] *F NEWLY IDENTIFIED GRs *F Gr2B1 *F MDTLRALEPLHRACQVCNLWPWRLAPPPDSEGILLRRSRWLELYGWTVLIAATSFTVYGLFQESSVEEKQD *F SESTISSIGHTVDFIQLVGMRVAHLAALLEALWQRQAQRGFFAELGEIDRLLSKALRVDVEAMRINMRRQTS *F RRAVWILWGYAVSQLLILGAKLLSRGDRFPIYWISYLLPLLVCGLRYFQIFNATQLVRQRLDVLLVALQQLQ *F LHQKGPAVDTVLEEQEDLEEAAMDRLIAVRLVYQRVWALVALLNRCYGLSMLMQVGNDFLAITSNCYWM *F FLNFRQSAASPFDILQIVASGVWSAPHLGNVLVLSLLCDRTAQCASRLALCLHQVSVDLRNESHNALITQFS *F LQLLHQRLHFSAAGFFNVDCTLLYTIVGATTTYLIILIQFHMSESTIGSDSNGQ *F Gr8D1 *F MSGHLGRVLQFHLRLYQVLGFHGLPLPGDGNPARTRRRLMAWSLFLLISLSALVLACLFSGEEFLYRGDMF *F GCANDALKYVFAELGVLAIYLETLSSQRHLANFWWLHFKLGGQKTGLVSLRSEFQQFCRYLIFLYAMMAA *F EVAIHLGLWQFQALTQHMLLFWSTYEPLVWLTYLRNLQFVLHLELLREQLTGLEREMGLLAEYSRFASETG *F RSFPGFESFLRRRLVQKQRIYSHVYDMLKCFQGAFNFSILAVLLTINIRIAVDCYFMYYSIYNNVINNDYYLIV *F PALLEIPAFIYASQSCMVVVPRIAHQLHNIVTDSGCCSCPDLSLQIQNFSLQLLHQPIRIDCLGLTILDCSLLTR *F MACSVGTYMIYSIQFIPKFSNTYM *F Gr10B1 *F MQRTHLEFEFKNAPQEPKRPFEFFMYFKFCLINLMMMIQVCGIFAQYGEVGKGSVSQVRVHFAIYAFVLWN *F YTENMADYCYFINGSVLKYYRQFNLQLGSLRDEMDGLRPGGMLLHHCCELSDRLEELRRRCREIHDLQRE *F SFRMHQFQLIGLMLSTLINNLTNFYTLFHMLAKQSLEEVSYPVVVGSVYATGFYIDTYIVALINEHIKLELEAV *F ALTMRRFAEPREMDERLTREVRNKIFSFLATTLEIMIQIWLSFFANFDDVTPYRKCENRPKNLFFKIRQKVIGI *F VSSGKLKLLVSLRFFIIDNRLILNLHKYLAIKLNFLNLIQIEHLSLELLNYQPPMLCGLLHLDRRLVYLIAVTAF *F SYFITLVQFDLYLRKKS *F Gr10B2 *F MRVGKLCRLALRFWMGLILVLGFSSHYYNPTRRRLVYSRILQTYDWLLMVINLGAFYLYYRYAMTYFLEG *F MFRRQGFVNQVSTCNVFQQLLMAVTGTWLHFLFERHVCQTYNELSRILKHDLKLKEHSRFYCLAFLAKVY *F NFFHNFNFALSAIMHWGLRPFNVWDLLANLYFVYNSLARDAILVAYVLLLLNLSEALRLNGQQEHDTYSDL *F MKQLRRRERLLRIGRRVHRMFAWLVAIALIYLVFFNTATIYLGYTMFIQKHDALGLRGRGLKMLLTVVSFL *F VILWDVVLLQVICEKLLAEENKICDCPEDVASSRTTYRQWEMSALRRAITRSSPENNVLGMFRMDMRCAFA *F LISCSLSYGIIIIQIGYIPG *F Gr28A2 *F MAFKLWERFSQADNVFQALRPLTFISLLGLAPFRLNLNPRKEVQTSKFSFFAGIVHFLFFVLCFGISVKEGDS *F IIGYFFQTNITRFSDGTLRLTGILAMSTIFGFAMFKRQRLVSIIQNNIVVDEIFVRLGMKLDYRRILLSSFLISLG *F MLLFNVIYLCVSYSLLVSATISPSFVTFTTFALPHINISLMVFKFLCTTDLARSRFSMLNEILQDILDAHIEQLS *F ALELSPMHSVVNHRRYSHRLRNLISTPMKRYSVTSVIRLNPEYAIKQVSNIHNLLCDICQTIEEYFTYPLLGIIA *F ISFLFILFDDFYILEAILNPKRLDVFEADEFFAFFLMQLIWYIVIIVLIVEGSSRTILHSSYTAAIVHKILNITDDPE *F LRDRLFRLSLQLSHRKVLFTAAGLFRLDRTLIFTVN FLQITGAATCYLIILIQF *F Gr28A4 *F MIRCGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSWYGFLNAIFRWLL *F LAYCYSYINLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPLLLRKYFLKSVKNMVQVDTQLERLRS *F PVNFNTVVGQVVLVILAVVLLDTVLLTTGLVCLAKMEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRI *F TCLHKFDFATMSALRRVRKYFISSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNIFIYIC *F LCGFCYISSLRQGESIVGYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFSNIGVRV *F KYSRIFRYSLLVLIFKLLILGVYFVGVFRLLVSLDVTPSFCVCMTFFLQ *F Gr33C1 *F MKRKAVEVIGLIPLNRQQSETNFILDYAMMCIVPIFYVACYLLINLSHIIGLCLLDSCNSVCKLSSHLFMHLGA *F FLYLTITLLSLYRRKEFFQQFDARLNDIDAVIQKCQRVAEMDKVKVTAVKHSVAYHFTWLFLFCVFTFALY *F YDVRSLYLTFGNLAFIPFMVSSFPYLAGSIIQGEFIYHVSVISQRFEQINMLLEKINQEARHRHAPLTVFDIESE *F GKKERKTVTPITVMDGRTTTGFGNENKFAGEMKRQEGQQKNDDDDLDTSNDEDEDDFDYDNATIAENTGN *F TSEANLPDLFKLHDKILALSVITNGEFGPQCVPYMAACFVVSIFGIFLETKVNFIVGGKSRLLDYMTYLYVIW *F SFTTMMVAYIVLRLCCNANNHSKQSAMIVHEIMQKKPAFMLSNDLFYNKMKSFTLQFLHWEGFFQFNGVG *F LFALDYTFIFSTVSAATSYLIVLLQFDMTAILRNEGLMS *F Gr36B2 *F MVDWVVLLLKAVHIYCYLIGLSNFEFDCRTGRVFKSRRCTIYAFMANIFILITIIYNFTAHGDTNLLFQSANKL *F HEYVIIIMSGLKIVALITVLNRWLQRGQMMQLVKDVIRLYMINPQLKSMIRWGILLKAFISFAIELLQVTLSVD *F ALDRQGTAEMMGLLVKLCVSFIMNLAISQHFLVILLIRAQYRIMNAKLRMVIEESRRLSFLQLRNGAFMTRC *F CYLSDQLEDIGEVQSQLQSMVGQLDEVFGMQGLMAYSEYYLSIVGTSYMSYSIYKYGPHNLKLSAKTSIIVC *F ILITLFYLDALVNCNNMLRVLDHHKDFLGLLEERTVFASSLDIRLEESVSFESLQLQLARNPLKINVMGMFPI *F TRGSTAAMCASVIVNSIFLIQFDME *F Gr36B3 *F MDLESFLLGAVYYYGLFIGLSNFEFDWNTGRVFTKKWSTLYAIALDSCIFALYIYHWTGNTNIVNAIFGRAN *F MLHEYVVAILTGLRIVTGLFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQ *F MAMVLSAMGSRVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKDLLLSP *F RHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVGTCYLAYSILKHGYENLS *F MTLSTVILAYSWCFFYYLDGMLNLSVMLHVQDDYWEMLQILGKRTIFVGLDVRLEEAVST *F Gr59C1 *F MIKLYFRYSLAIGITSQQFSNRKFFSTLFSRTYALIANIVTLIMLPIVMWQVQLVFQQKKTFPKLILITNNVREA *F VSFLVILYTVLSRGFRDTAFKEMQPLLLTLFREEKRCGFKGIGGVRRSLRILLFVKFFTLSWLCVTDVLFLLY *F STDALIWVNVLRFFFKCNTNNILEMVPMGYFLALWHIARGFDCVNRRLDQIVKSKSTRKHRELQHLWLLHA *F CLTKTALNINKIYAPQMLASRFDNFVNGVIQAYWGAVFTFDLSTPFFWVVYGSVQYHVRCLDYYLIDNMCD *F VAVEYHDSAKHSWSEVRWTKEVSAFGSILLYICMLMQLLSFQISSYVIYANSTKLQLWSCGLFQANRSMWF *F AMISSVLYYILVLLQFHLVMRK* *F Gr61D1 *F MSRTSDDIRKHLKVRRQKQRAILAMRWRCAQGGLEFEQLDTFYGAIRPYLCVAQFFGIMPLSNIRSRDPQD *F VKFKVRSIGLAVTGLFLLLGGMKTLVGANILFTEGLNAKNIVGLVFLIVGMVNWLNFVGFARSWSHIMLPW *F SSVDILMLFPPYKRGKRSLRSKVNVLALSVVVLAVGDHMLYYASGYCSYSMHILQCHTNHSRITFGLYLEK *F EFSDIMFIMPFNIFSMCYGFWLNGAFTFLWNFMDIFIVMTSIGLAQRFQQFAARVGALEGRHVPEALWYDIR *F RDHIRLCELASLVEASMSNIVFVSCANNVYVICNQALAIFTKLRHPINYVYFWYSLIFLLARTSLVFMTASKI *F HDASLLPLRSLYLVPSDGWTQEVQRFADQLTSEFVGLSGYRLFCLTRKSLFGMLATLVTYELMLLQIDAKS *F HKGLRCA *F Gr63F1 *F MRPSGEKVVKGHGQGNSGHSLSGMANYYRRKKGDAVFLNAKPLNSANAQAYLYGVRKYSIGLAERLDAD *F YEAPPLDRKKSSDSTASNNPEFKPSVFYRNIDPINWFLRIIGVLPIVRHGPARAKFEMNSASFIYSVVFFVLLA *F CYVGYVANNRIHIVRSLSGPFEEAVIAYLFLVNILPIMIIPILWYEARKIAKLFNDWDDFEVLYYQISGHSLPLK *F LRQKAVYIAIVLPILSVLSVVITHVTMSDLNINQVVPYCILDNLTAMLGAWWFLICEAMSITAHLLAERFQKA *F LKHIGPAAMVADYRVLWLRLSKLTRDTGNALCYTFVFMSLYLFFIITLSIYGLMSQLSEGFGIKDIGLTITAL *F WNIGLLFYICDEAHYASVNVRTNFQKKLLMVELNWMNSDAQTEINMFLRATEMNPSTINCGGFFDVNRTLF *F KGLLTTMVTYLVVLLQFQISIPTDKGDSEGANNITVVDFVMDSLDNDMSLMGASTLSTTTVGTTLPPPIMKL *F KGRKG *F Gr64A2 *F MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFYLSIFLISLSFFQL *F ARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLVEHIMSMLSMGYYVNSCPRWPDRPID *F SFLYLSFSSVFYFVDYTRFLGIVGKVVNVLSTFAWNFNDIFVMAVSVALAARFRQLNDYMMREARLPTTV *F DYWMQCRINFRNLCKLCEEVDDAISTITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLIL *F SLYSSSINDESKRPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILLQ *F FNGEEK *F Gr64A3 *F MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSLLFIASSLANFG *F LSLFKVLNNPISFNSIKPIIFRGSVLLVLIVALNLARQWPQLMMYWHTVEKDLPQYKTQLTKWKMGHTISMV *F MLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYFLRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYM *F DLFVMIVSIGLASKFRQLNDDLRNFKGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFIC *F VQLLRSLNTMPSVAHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD *F EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS *F Gr66C1 *F MDNMAQAEDAVQPLLQQFQQLFFISKIAGILPQDLEKFRSRNLLEKSRNGMIYMLSTLILYVVLYNILIYSFG *F EEDRSLKASQSTLTFVIGLFLTYIGLIMMVSDQLTALRNQGRIGELYERIRLVDERLYKEGCVMDNSTIGRRIR *F IMLIMTVIFELSILVSTYVKLVDYSQWMSLLWIVSAIPTFINTLDKIWFAVSLYALKERFEAINATLEELVDTHE *F KHKLWLRGNQEVPPPLDSSQPPQYDSNLEYLYKELGAIDAASRKPPPPPLATNMVHESELGNAAKVEEKLN *F NLCQVHDEICEIGKALNELWSYPILSLMAYGFLIFTAQLYFLYCATQYQSIPSLFRSAKNPFITVIVLSYTSGK *F CVYLIYLSWKTSQASKRTGISLHKCGVVADDNLLYEIVNHLSLKLLNHSVDFSACGFFTLDMETLYGVSGGI *F TSYLIILIQFNLAAQQAKEAIQTFNSLNDTAGLVGAATDMDNISSTLRDFVTTTMTPAV *F Gr92D1 *F MFEFLHQMSAPKLSTSILRYIFRYAQFIGVIFFCLHTRKDDKTVFIRNWLKWLNVTHRIITFTRFFWVYIASISI *F KTNRVLQVLHGMRLVLSIPNVAVILCYHIFRGPEIIDLINQFLRLFRQVSDLFKTKTPGFGGRRELILILLNLISF *F AHEQTYLWFTIRKGFSWRFLIDWWCDFYLVSATNIFIHINSIGYLSLGVLYSELNKYVYTNLRIQLQKLNTSG *F SKQKIRRVQNRLEKCISLYREIYHTSIMFHKLFVPLLFLALIYKVLLIALIGFNVAVEFYLNSFIFWILLGKHVL *F DLFLVTVSVEGAVNQFLNIGMQFGNVGDLSKFQTTVSQFIFIDFIPI *F Gr98A1 *F MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFIVSYFNIIAIDEEV *F LEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIADLEMDMDEASQCFGGQRQRFSFRFR *F MALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILTEFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQL *F QEEFQDCEQQDMLQALCVALKRNQLLLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYD *F SCCQYGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVDEIGAYFR *F WSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLMSFHFSLNMEANRSRLLAAARPYIQIYSIFGLTPPIQFFT *F RTLHKRRRGIVILGYACYLISISLMVIYECYANIVALQKDIHKFHAEDSSKVMGNTQKVLVVAMFVWNQLNI *F LLNFRRLARIYDDIADLEIDLNNASSGFVGQRHWWRFRFRLALSVGLWIVLLVGLTPRFTLVALGPYLHWTN *F KVLTEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQLLAGRIWGLVN *F EVSLYFTLSLTLLFLYNELTILQIVNWALIKSVNPNECCQYTEDYLILKMGLREYSLQMEHLKLIFTCGGLFDI *F NLKFFGGVKLKL *F Gr98A2 *F MEAKRSRLLTTARPYLQVLSLFGLTPPAEFFTRTLRKRRRFCWMAGYSLYLIAILLMVFYEFHANIVSLHLEI *F YKFHVEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIANLDLGIDKSSKNFCGKSHWWSFRLRLT *F LSIGLWMVIIIGVIPRLTLGRAGPFFHWVNQVLTQIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLED *F FDCGARIQELCVTLKQNQLLIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE *F Gr2940.1 *F MFRPSGSGYRQKWTGLTLKGALYGSWILGVFPFAYDSWTRTLRRSKWLIAYGFVLNAAFILLVVTNDTESE *F TPLRMEVFHRNALAEQINGIHDIQSLSMVSIMLLRSFWKSGDIERTLNELEDLQHRYFRNYSLEECISFDRFV *F LYKGFSVVLELVSMLVLELGMSPNYSAQFFIGLGSLCLMLLAVLLGASHFHLAVVFVYRYVWIVNRELLKL *F VNKMAIGETVESERMDLLLYLYHRLLDLGQRLASIYDYQMVMVMVSFLIANVLGIYFFIIYSISLNKSLDFKI *F LVFVQALVINMLDFWLNVEICELAERTGRQTSTILKLFNDIENIDEKLERSVSFTSQHYCETDFALFCSHRRL *F RFHHCGLFYVNYEMGFRMAITSFLYLLFLIQFDYWNL *F Gr2940.2 *F MVKQAEDREHGIMLDVFQRNALLYQISSLMGVVGVVSICTVHLRTLWRSKHLEEIYNGLMLLEAKYFCSNA *F VECPAFDGYVIQKGVVIVVGLLAPWMVHFGMPDSKLPVLNVLVVSMVKLGTLLLALHYHLGVVIIYRFVWL *F INRELLSLVCSLRGNHKGSSSRVRFLLKLYNKLVNLYSKLADCYDCQTVLMMAIFLAANIIVCFYMIVYRISL *F SKMSFFVMLIMFPLAIANNFMDFWLSMKVCDLLQKTGRQTSMILKLFNDIENMDKDLEISISDFALYCSHRR *F FKFLHCGLFHVNREMGFKMFVASVLYLLYLVQF *F Gr2940.3 *F MFASRSDLQSRLCWIILKATLYSSWFLGVFPYRFDSRNGQLKRSRFLLFYGLILNFFLLLKMVCSGGQKLGI *F PEAFARNSVLENTHYTTGMLAVFSCVVIHFLNFWGSTRVQDLANELLVLEYQQFASLNETKCPKFNSFVIQK *F WLSVIGLLLSYLSIAYGLPGNNFSVEMVLINSLVQFSFNCNIMHYYIGVLLIYRYLWLINGQLLEMVTNLKLD *F CSVDSSRIRKYLSLYRRLLELKGYMVATYEYHMTLVLTTGLASNFLAIYSWIVLDISMNINFIYLLIFPLFLLV *F NVWNLWLSIAASDLAENAGKSTQTVLKLFADLEVKDIELERSVSVNSNRYKQVNEFALLCGHCQFNFHVCG *F LFTINYKMGFQMIITSFLYLIYMIQFD *F Gr2940.4 *F MINVVIGIINVLSALIVHFMNFWGSRKVGEICNELLILEYQDFEGLNGRNCPNFNCFVIQKCLTILGQLLSFFTL *F NFALPGLEFHICLVLLSCLMEFSLNLNIMHYHVGVLLIYRYVWLINEQLKDLVSQLKLNPETDFSRIHQFLSL *F YKRLLELNRKLVIAYEYQMTLFIIAQLSGNIVVIYFLIVYGLSMRTYSIFLVAFPNSLLINIWDFWLCIAACDLT *F EKAGDETAIILKIFSDLEHRDDKLEKFRFQLCGLFSMNCRMGFKMIITTFLYLVYLVQFDYMNL* *F Gr2940.5 *F MSQPKRIHRICKGLARFTIRATLYGSWVLGLFPFTFDSRKRRLNRSKWLLAYGLVLNLTLLVLSMLPSTDDH *F NSVKVEVFQRNPLVKQVEELVEVISLITTLVTHLRTFSRSSELVEILNELLVLDKNHFSKLMLSECHTFNRYVI *F EKGLVIILEIGSSLVLYFGIPNSKIVVYEAVCIYIVQLEVLMVVMHFHLAVIYIYRYLWIINGQLLDMASRLRRG *F DSVDPDRIQLLLWLYSRLLDLNHRLTAIYDIQVTLFMATLFSVNIIVGHVLVICWINITRFSLLVIFLLFPQALII *F NFWDLWQGIAFCDLAESTGKKTSMILKLFNDMENMDQETERRVSEYMFQNLMYFKYFKHPLIFVAEFTLF *F CSHRRLKVCHLGLLDINYEMGFRMIITNILYVVFLVQFDYMNL *F PREVIOUSLY REPORTED GRs (Clyne, et al. 2000) *F Gr21D1 *F MGVMPIHRNPPEKNLPRTGYSWGSKQVMWAIFIYSCQTTIVVLVLRERVKKFVTSPDKRFDEAIYNVIFISLL *F FTNFLLPVASWRHGPQVAIFKNMWTNYQYKFFKTTGSPIVFPNLYPLTWSLCVFSWLLSIAINLSQYFLQPD *F FRLWYTFAYYPIIAMLNCFCSLWYINCNAFGTASRALSDALQTTIRGEKPAQKLTEYRHLWVDLSHMMQQL *F GRAYSNMYGMYCLVIFFTTIIATYGSISEIIDHGATYKEVGLFVIVFYCMGLLYIICNEAHYASRKVGLDFQTK *F LLNINLTAVDAATQKEVEMLLVAINKNPPIMNLDGYANINRELITTNISFMATYLVVLLQFKITEQRRIGQQQA *F Gr22B1 *F MFQPRRGFSCHLAWFMLQTTLYASWLLGLFPFTFDSRRKQLKRSRWLLLYGFVLHSLAMCLAMSSHLAS *F KQRRKYNAFERNPLLEKIYMQFQVTTFFTISVLLLMNVWKSNTVRKIANELLTLEGQVKDLLTLKNCPNFNC *F FVIKKHVAAIGQFVISIYFCLCQENSYPKILKILCCLPSVGLQLIIMHFHTEIILVYRYVWLVNETLEDSHHLSSS *F RIHALASLYDRLLKLSELVVACNDLQLILMLIIYLIGNTVQIFFLIVLGVSMNKRYIYLVASPQLIINFWDFWLN *F IVVCDLAGKCGDQTSKVLKLFTDLEHDDEELERS *F LNEFAWLCTHRKFRFQLCGLFSINHNMGFQMIITSFLYLVYLLQFDFMNLC *F Gr23A1a *F MKTLECLTRRFLEVIFSVLALVPLPPISQLGWLFLSLAIRCCWIVYFIYLLDVAISFSWVAIENVGNAVGTMLF *F VGNSVLGFALLLESVLKQKTHSQLEDLRVQTELQLQRLGMFGRSRHAAYLLPLIGVQFTCDLVRLATNFGE *F TVSPVFCISLPLMWLLRYRYVQLVQHVMDLNQRSIHLRRSLLSMASGNDLWQPYGVQECLQLQTLRTTYE *F RIFECYETFSDCYGWGMLGLHLLTSFQFVTNAYWMIMGIYDGGNVRSLIFNGATGIDFGTPIATLFWHGDSG *F AENGRQIGCLISKLVKPQGSKLYNDLVSEFSLQTLHQRFVVTAKDFFSLNLHLLSSMFAAVVTYLVILIQFMF *F AERSSTRGSG *F Gr23A1b *F MFPPTRVQASSRVVLKIFHFILVAFSLRSRRLSRLVLWLQFLGWLTWFISMWTQSVIYAQTIDCTLDCSLRHI *F LTFFQTVSHAFIVVTSFLDGFRIKQDQLDEPIAFEDSDPWLAFTVLAMLVPTLGVEYLVCSNAPEYAFRIRIY *F HLKTLPSFLALQVQIISFILEVMKVNIRVRQTKLQLLILARELSCRWPQRKQKPQFSDQQAHRVKDLKRRYN *F DLHYLFVRINGYFGGSLLTIIIVHFAIFVSNSYWLFVDIRTRPWRIYAILLNLGFIFNVALQMAAACWHCQQSY *F NLGRQIGCLISKLVKPQGSKLYNDLVSEFSLQTLHQRFVVTAKDFFSLNLHLLSSMFAAVVTYLVILIQFMFA *F ERSSTRGSG *F Gr32D1 *F MPIYEQVSDYEVGPPTKTNEFYSFFVRGVVHALTIFNVYSLFTPISAQLFFSYRETDNVNQWIELLLCILTYTL *F TVFVCAHNTTSMLRIMNEILQLDEEVRRQFGANLSQNFGFLVKFLVGITACQAYIIVLKIYAVQGEITPTSYIL *F LAFYGIQNGLTATYIVFASALLRIVYIRFHFINQLLNGYTYGQQHRRKEGGARARRQRGDVNPNVNPALMEH *F FPEDSLFIYRMHNKLLRIYKGINDCCNLILVSFLGYSFYTVTTNCYNLFVQITGKGMVSPNILQWCFAWLCLH *F VSLLALLSRSCGLTTTEVSNYIGDKISIFMSVFISRPMPHPKFLQGCMPSRRSIRISGFHYQIDKFLTKSIKQEV *F QFTAYGFFAIDNSTLFKIFSAVTTYLVILIQFKQLEDSKVEDPVPEQT *F Gr39D1 *F MLYSFHPYLKYFALLGLVPWSESCAQSKFVQKVYSAILIILNAVHFGISIYFPQSAELFLSLMVNVIVFVARIV *F CVTVIILQVMVHYDDYFRFCREMKYLGLRLQCELKIHVGRLKWQSYAKILALGIGFLVTVLPSIYVALSGSLL *F YFWSSLLSILIIRMQFVLVLLNVELLGHHVSLLGIRLQNVLECHLMGANCTLDGNANRLCSLEFLLALKQSH *F MQLHYLFTHFNDLFGWSILGTYVVLFSDSTVNIYWTQQVLVEVYEYKYLYATFSVFVPSFFNILVFCRCGEF *F CQRQSVLIGSYLRNLSCHPSIGRETSYKDLLMEFILQVEQNVLAINAEGFMSTDNSLLMS *F ILAAKVTYLIVLMQFSSV *F Gr39D2a *F MGTRNRKLLFFLHYQRYLGLTNLDFSKSLHIYWLHGTWSSTAIQIVVVGVFMAALLGALAESLYYMETKSQ *F TGNTFDNAVILTTSVTQLLANLWLRSQQKSQVNLLQRLSQVVELLQFEPYAVPQFRWLYRIWLLVCLIYGA *F MVTHFGINWLTTMQISRVLTLIGFVYRCVLANFQFTCYTGMVVILKKLLQVQVKQLEHLVSTTTISMAGVAG *F CLRTHDEILLLGQRELIAVYGGVILFLFIYQVMQCILIFYISNLEGFHSSNDLVLIFCWLAPMLFYLILPLVVNDI *F HNQANKTAKMLTKVPRTGTGLDRMIEKFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEM *F ENSTKSINKF *F Gr39D2b *F MDFQPGELCAYYRLCRYLGIFCIDYNPTKKKFRLRRSVLCYIVHFALQAYLVGCISVMVTYWRRCFKSELT *F TTGNHFDRLVMVIALGILVVQNAWLIWLQAPHLRIVRQIEFYRRNHLANVRLLLPKRLLWLIIATNVVYMAN *F FIKTCIFEWLTDASRLFVITSLGFPLRYLVTSFTMGTYFCMVHIVRLVLDWNQSQINAIIDESADLKMTSPNRL *F RLRVCLEMHDRLMLLCNDEISLVYGFIAWLSWMFASLDVTGVIYLTMVIQTKKSIVLKLITNVVWLSPTFMT *F CAASFMSNRVTIQANKTAKMLTKVPRTGTGLDRMIEKFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTY *F MVILVQFKEMENSTKSINKF *F Gr39D2c *F MKRNAFEELRVQLRTLKWLGVLRFTIDFNKCLVRENASEERSAWLYLIGVVGITCSLIVYSTYFPSHFIMGK *F HNTTGNCYALINIRSCSIVTMLIYTQLYIQRFRFVALLQSILRFNQISGSHREEGRFAFYYYTHLSLLIICMLNY *F AYGYWTAGVRLTTIPIYLLQYGFSYLFLGQVVVLFACIQQILLSILKYYNQVVLKNIKSSKESREFYYNFCKY *F NQVIWLSYTEINHCFGLLLLLVTGLILLITPSGPFYLVSTIFEGRFRQNWQFSLMSFTAILWSLPWIVLLVLAM *F GRNDVQKEANKTAKMLTKVPRTGTGLDRMIEKFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILV *F QFKEMENSTKSINKF *F Gr39D2d *F MSKVCRDLRIYLRLLHIMGMMCWHFDSDHCQLVATSGSERYAVVYAGCILVSTTAGFIFALLHPSRFHIAIY *F NQTGNFYEAVIFRSTCVVLFLVYVILYAWRHRYRDLVQHILRLNRRCASSCTNQQFLHNIILYGMLTILCFGN *F YLHGYTRAGLATLPLALCMLVYIFAFLVLCLLLMFFVSLKQVMTAGLIHYNQQLCQGDLISGLRGRQQILKL *F CGGELNECFGLLMLPIVALVLLMAPSGPFFLISTVLEGKFRPDECLIMLLTSSTWDTPWMIMLVLMLRTNGI *F SEEANKTAKMLTKVPRTGTGLDRMIEKFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEM *F ENSTKSINKF *F Gr43C1 *F MKSATSKVVTALDVSVVVMAIVSGVYCGLFSLNDTLELNDRLNKIDNTLNAYNNFRRDRWRALGMAAVSL *F LAISILVGLDVGTWMRIAQDMNIAQSDTELNVHWYIPFYSLYFILTGLQVNIANTAYGLGRRFGRLNRMLSSS *F FLAENNATSAIKPQKVSTVKNVSVNRPAMPSALHASLTKLNGETLPSEAAGDKAAARSLILNVELLKLGYFP *F AKNKGLLLKSLADSHESLGKCVHLLSNSFGIAVLFILVSCLLHLVATAYFLFLELLSKRDNGYLWVQMLWIC *F FHFLRLLMVVEPCHLAARESRKTIQIVCEIERKVHEPILAEAVKKFWQQLLVVDADFSACGLCRVNRTILTSF *F ASAIATYLVILIQFQRTNG *F Gr47A1 *F MAFTSSQLCSLLTKFTALNGLNTYYFDTKTNAFRVSSKLKIYCAIHHALCVLALAHMSYSTASNLRVSVTVL *F TIGGTMACCVKSCWEKAQGIRNLARGLVTMEQKYFAGRPSGLLLKCRYYIKITFGSITLLRIHLIQPIYMRRL *F LPSQFYLNVGAYWLLYNMLLAAVLGFYFLLWEMCRIQKLINDQMTLILARSGQRNRLKKMQHCLRLYSKL *F LLLCDQFNSQLGHVAIWVLACKSWCQITFGYEIFQMVAAPKSIDLTMSMRVFVIFTYIFDAMNLFLGTDISEL *F FSTFRADSQRILRETSRLDRLLSMFALKLALHPKRVVLLNVFTFDRKLTLTLLAKSTLYTICCLQNDYNKLKA *F Gr58A1 *F MLLKFMYIYGIGCGLMPAPLKKGQFLLGYKQRWYLIYTACLHGGLLTVLPFTFPHYMYDDSYMSSNPVLK *F WTFNLTNITRIMAMFSGVLLMWFRRKRILNLGENLILHCLKCKTLDNRSKKYSKLRKRVRNVLFQMLLVANLSILLG *F ALILFRIHSVQRISKTAMIVAHITQFIYVVFMMTGICVILLVLHWQSERLQIALKDLCSFLNHEERNSLTLSENK *F ANRSLGKLAKLFKLFAENQRLVREVFRTFDLPIALLLLKMFVTNVNLVYHGVQFGNDTIETSSYTRIVGQWV *F VISHYWSAVLLMNVVDDVTRRSDLKMGDLLREFSHLELVKRDFHLQLELFSDHLRCHPSTYKVCGLFIFNK *F QTSLAYFFYVLVQVLVLVQFDLKNKVEKRN *F Gr58A2 *F MLHPKLGRVMNVVYYHSVVFALMSTTLRIRSCRKCLRLEKVSRTYTIYSFFVGIFLFLNLYFMVPRIMEDG *F YMKYNIVLQWNFFVMLFLRAIAVVSCYGTLWLKRHKIIQLYKYSLIYWKRFGHITRAIVDKKELLDLQESLA *F RIMIRKIILLYSAFLCSTVLQYQLLSVINPQIFLAFCARLTHFLHFLCVKMGFFGVLVLLNHQFLVIHLAINALH *F GRKARKKWKALRSVAAMHLKTLRLARRIFDMFDIANATVFINMFMTAINILYHAVQYSNSSIKSNGWGILF *F GNGLIVFNFWGTMALMEMLDSVVTSCNNTGQQLRQLSDLPKVGPKMQRELDYFTMQLRQNRLVYKICGIV *F ELDKPACLSYIGSILSNVIILMQFDLRRQRQPINDRQYLIHLMKNKTKV *F Gr58A3 *F MNQYFLLHTYFQVSRLIGLCNLHYDSSNHRFILNHVPTVVYCVILNVVYLLVLPFALFVLTGNIYHCPDAGM *F FGVVYNVVALTKLLTMLFLMSSVWIQRRRLYKLGNDLMKMLHKFRFNLGNDCRNRCLCKGLLTSSRFVL *F LTQQLLTRDSVVNCESNSSLRQAMVPYQSAAIVYALIMILLMSYVDMTVYMVEVAGNWLLVNMTQGVRE *F MVQDLEVLPERNGIPREMGLMQILAAWRKLWRRCRRLDALLKQFVDIFQWQVLFNLLTTYIFSIAVLFRLWI *F YLEFDKNFHLWKGILYAIIFLTHHVEIVMQFSIFEINRCKWLGLLEDVGNLWDINYSGRQCIKSSGTILSRKLE *F FSLLYMNRKLQLNPKRVRRLHIVGLFDISNLTVHNMTRSIITNVLVLCQIAYKKYG *F Gr59D1 *F MADLLKLCLRIAYAYGRLTGVINFKIDLKTGQALVTRGATLISVSTHLLIFALLLYQTMRKSVVNVMWKYAN *F SLHEYVFLVIAGFRVVCVFLELVSRWSQRRTFVRLFNSFRRLYQRNPDIIQYCRRSIVSKFFCVTMTETLHII *F VTLAMMRNRLSIALALRIWAVLSLTAIINVIITQYYVATACVRGRYALLNKDLQAIVTESQSLVPNGGGVFVT *F KCCYLADRLERIAKSQSDLQELVENLSTAYEGEVVCLVITYYLNMLGTSYLLFSISKYGNFGNNLLVIITLCGI *F VYFVFYVVDCWINAFNVFYLLDAHDKMVKLLNKRTLFQPGLDHRLEMVFENFALNLVRNPLKLHMYGLFE *F FGRGTSFAVFNSLLTHSLLLIQYDVQNF *F Gr59D2 *F MVDLVKTILLIAYWYGLAVGVSNFEVDWLTGEAIATRRTTIYAAVHNASLITLLILFNLGNNSLKSEFISARYL *F HEYFFMLMTAVRISAVLLSLITRWYQRSRFIRIWNQILALVRDRPQVVRGRWYRRSIILKFVFCVLSDSLHTI *F SDVSAQRKRITADLIVKLSLLATLTTIFNMIVCQYYLAMVQVIGLYKILLQDLRCLVRQAECICSIRNRRGGV *F YSIQCCSLADQLDLIAERHYFLKDRLDEMSDLFQIQSLSMSLVYFFSTMGSIYFSVCSILYSSTGFGSTYWGL *F LLIVLSTASFYMDNWLSVNIGFHIRDQQDELFRVLADRTLFYRELDNRLEAAFENFQLQLASNRHEFYVMGL *F FKMERGRLIAMLSSVITHTMVLVQWEIQN *F Gr59E1 *F MRSSATKGAKLKNSPRERLSSFNPQYAERYKELYRTLFWLLLISVLANTAPITILPGCPNRFYRLVHLSWMI *F LWYGLFVLGSYWEFVLVTTQRVSLDRYLNAIESAIYVVHIFSIMLLTWQCRNWAPKLMTNIVTSDLNRAYTI *F DCNRTKRFIRLQLFLVGIFACLAIFFNIWTHKFVVYRSILSINSYVMPNIISSISFAQYYLLLQGIAWRQRRLTE *F GLERELTHLHSPRISEVQKIRMHHANLIDFTKAVNRTFQYSILLLFVGCFLNFNLVLFLVYQGIENPSMADFT *F KWVCMLLWLAMHVGKVCSILHFNQSIQNEHSTCLTLLSRVSYARKDIQDTITHFIIQMRTNVRQHVVCGVIN *F LDLKFLTTLLVASADFFIFLLQYDVTYEALSKSVQGNVTRY *F Gr59E2 *F MDSSYWENLLLTINRFLGVYPSGRVGVLRWLHTLWSLFLLMYIWTGSIVKCLEFTVEIPTIEKLLYLMEFPG *F NMATIAILVYYAVLNRPLAHGAELQIERIITGLKGKAKRLVYKRHGQRTLHLMATTLVFHGLCVLVDVVNYD *F FEFWTTWSSNSVYNLPGLMMSLGVLQYAQPVHFLWLVMDQMRMCLKELKLLQRPPQGSTKLDACYESAF *F AVLVDAGGGSALMIEEMRYTCNLIEQVHSQFLLRFGLYLVLNLLNSLVSICVELYLIFNFFETPLWEESVLLV *F YRLLWLAMHGGRIWFILSVNEQILEQKCNLCQLLNELEVCSSRLQRTINRFLLQLQRSIDQPLEACGIVTLDT *F RSLGGFIGVLMAIVIFLIQIGLGNKSLMGVALNRSNWVYV *F Gr1F1 *F Could not identify in AL035632, 7301-8711 *F Gr2B is in AL035632, 2549..3532,3599..3667,3722..3811,3861..3950 *F Gr47F1 *F Could not identify inAC005653, 42838-44204 *F Gr68D1 *F MKIYQDIYPISKPSQIFAILPFYSGDVDDGFRFGGLGRWYGRLVALIILIGSLTLGEDVLFASKEYRLVASAQG *F DTEEINRTIETLLCIISYTMVVLSSVQNASRHFRTLHDIAKIDEYLLANGFRETYSCRNLTILVTSAAGGVLAV *F AFYYIHYRSGIGAKRQIILLLIYFLQLLYSTLLALYLRTLMMNLAQRIGFLNQKLDTFNLQDCGHMENWRELS *F NLIEVLCKFRYITENINCVAGVSLLFYFGFSFYTVTNQSYLAFATLTAGSLSSKTEVADTIGLSCIWVLAETIT *F MIVICSACDGLASEVNGTAQILARIYGKSKQFQNLIDKFLTKSIKQDLQFTAYGFFSIDNSTLFKIFSAVTTYLV *F ILIQFKQLEDSKNLSRSYQLVM *F Gr77E1 *F MPRWLQLPGMSALGILYSLTRVFGLMATANWSPRGIKRVRQSLYLRIHGCVMLIFVGCFSPFAFWCIFQRM *F AFLRQNRILLMIGFNRYVLLLVCAFMTLWIHCFKQAEIIGCLNRLLKCRRRLRRLMHTRKLKDSMDCLATK *F GHLLEVVVLLSSYLLSMAQPIQILKDDPEVRRNFMYACSLVFVSVCQAILQLSLGMYTMAILFLGHLVRHSN *F LLLAKILADAEHIFESSQKAGFWPNRQELYKGQQKWLALELWRLLHVHHQLLKLHRSICSLCAVQAVCFLG *F FVPLECTIHLFFTYFMKYSKFILRKYGRSFPLNYFAIAFLVGLFTNLLLVILPTYYSERRFNCTREIIKGGGLAF *F PSRITVKQLRHTMHFYGLYLKNVEHVFAVSACGLFKLNNAILFCIVGAILEYLMILIQFDKVLN *F PREVIOUSLY REPORTED PARTIAL GR SEQUENCES *F Predicted proteins are extended where possible; extended sequence information *F is highlighted *F in black *F Gr28A1 *F Gr57B1 *F MAVLYFFREPETVFDCAAFICILQFLMGCNGFGIRRSTFRISWASRIYSMSVAIAAFCCLFGS *F LSVLLAEEDIRERLAKADNLVLSISALELLMSTLVFGVTVISLQVFARRHLGIYQRLAALDAR *F LMSDFGANLNYRKMLRKNIAVLGIVTTIYLMAINSAAVQVASGHRALFLLFALCYTIVTGGPHFTG *F YVHMTLAEMLGIRFRLLQQLLQPEFLNWRFPQLHVQELRIRQVVSMIQELHYLIQEINRVYALSLWAAMAH *F DLAMSTSELYILFGQSVGIGQQNEEENGSCYRMLGYLALVMIPPLYKLLIAPFYCDRTIYEARRCLRLVEKLD *F DWFPQKSSLRPLVESLMSWRIQAKIQFTSGLDVVLSRKVIGLFTSILVNYLLILIQFAMTQKMG *F EQIEQQKIALQEWIGF *F Gr65C1 *F MRVHQRQSAVIIQMGHPPFMSLKGGKSGFGSIVWPSAMREVNLLNRFTRQFLFLIVLVTQICGVATFVYNS *F KAQCFRQSGFLRFYSSLVLIFLALFLIVTTSKMFHNLQAVWPYVVGSVIILVVRIHGLLESAEIVELLNQMLRI *F MRQVNLMARHPNLFRLKHLLLLLLALQNLLRSLNTIVGISNHSAEAYDSFLNSVILLIILAVLLSFLLQITINIC *F LFVVLIATYSELHHCTRRISNDMDKLRLHSVHESGQFMVLVKQLQGITEKLIRLRQNVFHITVRIIRHFRFHW *F LCAIIYGLLPFFSLTAKDQNGFNFLIISALNIIFQWTIFAILSRES *F Gr93F1 *F MTGKRAESWSRLLLLWLYRCARGLLVLSSSLDRDKLQLKATKQGSRNRFLHILWRCIVVMI *F YAGLWPMLTSAVIGKRLESYADVLALAQSMSVSILAVISFVIQARGENQFREVLNRYLALYQ *F RICLTTRLRHLFPTKFVVFFLLKLFFTLCGCFHEIIPLFENSHFDDISQMVGTGFGIYMWLGTL *F CVLDACFLGFLVSGILYEHMANNIIAMLKRMEPIESQDERYRMTKYRRMQLLCDFADELDE *F CAAIYSELYHVTNSFRRILQWQILFYIYLNFINICLMLYQYILHFLNDDEVVFVSIVMAFVKLA *F NLVLLMMCADYTVRQSEVPKKLPLDIVCSDMDERWDKSVSLLLFETFLGQLQTQRLEIKVLGFF *F HLNNEFILLILSAIISYLFILIQFGITGGFEASEDIKNFAD *F Gr93F2 *F MQFWFGEELINLVNRFLQLFRRMQSLTNSPKNRFGDRAEFLLMFSKVFSLLFVFMAFRLML *F SPWFLLTLVCDLYTSVGTGMITHLCFVGYLSIGVLYRDLNNYVDCQLRAQLRSLNGENNSFR *F NNPQPTRQAISNLDKCLYLYDEIHQVSRSFQQLFDLPLFLSLAQSLLAMSMVSYHAILRRQY *F SFNLWGLVIKLLIDVVLLTMSVHSAVNGSRLIRRLSFENFYVTDSQSYHQKVSPGAIILRIKYN *F TFPILQLELFLGRLQHQELRVFPLGLFEVSNELTLFFLSAMVTYLVFLVQ *F Gr93F3 *F MIERLKKVSLPALSAFILFCSCHYGRILGVICFDIGQRTSDDSLVVRNRHQFKWFCLSCRLIS *F VTAVCCFCAPYVADIEDPYERLLQCFRLSASLICGICIIVVQVCYEKELLRMIISFLRLFRRVR *F RLSSLKRIGFGGKREFFLLLFKFICLVYELYSEICQLWHLPDSLSLFATLCEIFLEIGSLMIIHIG *F FVGYLSVAALYSEVNSFARIELRRQLRSLERPVGGPVGRKQLRIVEYRVDECISVYDEIERVG *F RTFHRLLELPVLIILLGKIFATTILSYEVIIRPELYARKIGMWGLVVKSFADVILLTLAVHEAVS *F SSRMMRRLSLENFPITDHKAWHMKVSDLMVFLIKCIFFSRLQWEMFLSRLNFFEFRVRPLGLFE *F VSNEVILLFLSSMITYFTYVVQ *F Gr93F4 *F MSFYARFLSLVCFRLRKQKDNNVWLEEIWSNRSRWKWISVTLRIVPLCIYAFTYAEWISNRM *F LITEKFLHSCSLVVSIPCYLSIIHLKICHGPEVTKLVNQYLHIFRLGTLDIRRRSQFGGGRELFLLILSVCCQ *F IHEYVFILVIASRLCGFQHIIWWVSYTYVFIICNSIMCFGFIWHLSLGVLYAELNDNLRFESGFQTAFLRKQQRI *F RVQKSMALFKEISSVVTSLQDIFNVHLFLSALLTLLQVLVVWYKMIIDLGFSDFRIWSFSLKNLIQTLLPVLAI *F QEAANQFKQTRERALDIFLVGKSKHWMKSVSKLINQGILQLIGLFNVSNELFLIIVSAMFCYLVFVTQC *F VIVYRRRYVI *F Gr94E1 *F MDFTSDYAHRRMVKFLTIILIGFMTVFGLLANRYRAGRRERFRFSKANLAFASLWAIAFSLVYGRQ *F IYKEYQEGQINLKDATTLYSYMNITVAVINYVSQMIISDHVAKVLSKVPFFDTLKEFRLDSRSLYI *F SIVLALVKTVAFPLTIEVAFILQQRRQHPEMSLIWTLYRLFPLIISNFLNNCYFGAMVVVKEILYA *F LNRRLEAQLQEVNLLQRKDQLKLYTKYYRMQRFCALADELDQLAYRYRLIYVHSGKYLTPMSLSMI *F LSLICHLLGITVGFYSLYYAIADTLIMGKPYDGLGSLINLVFLSISLAEITLLTHLCNHLLVATRR *F SAVILQEMNLQHADSRYRQAVHGFTLLVTVTKYQIKPLGLYELDMRLISNVFSAVASFLLILVQADLSQRFKMQ *F Gr97D1 *F MRFLRRQTRRLRSIWQRSLPVRFRRGKLHTQLVTICLYATVFLNILYGVYLGRFSFRRKKFVFSKGLTI *F YSLFVATFFALFYIWNIYNEISTGQINLRDTIGIYCYMNVCVCLFNYVTQWEKTLQIIRFQNSVPLFKVLDSLDI *F SAMIVWRAFIYGLLKIVFCPLITYITLILYHRRSISESQWTSVTTTKTMLPLIVSNQINNCFFGGLVLANLIFAA *F VNRKLHGIVKEANMLQSPVQMNLHKPYYRMRRFCELADLLDELARKYGFTASRSKNYLRFTDWS *F MVLSMLMNLLGITMGCYNQYLAIADHYINEEPFDLFLAIVLVVFLAVPFLELVMVARISNQT *F LVEVIVI *F Gr98B1 *F Gr98B2 *F AC007817 10695-10784 contains no sequence information *F Gr98B3 *F AC007817 45189-45284 contains no open reading frame *F Gr98B4 *F AC007817 39658-39765 contain no open reading frame *F GrLU1=Gr36B1 *F MFDWVGLLLKVLYYYGQIIGLINFEIDWQRGRVVAAQRGILFAIAINVLICMVLLLQISKKFNL *F DVYFGRANQLHQYVIIVMVSLRMASLNRWRQRAQLMRLVECVLRLFLKKPHVKQMSRWAILVKF *F SVGVVSNFLQMAISMESLDRLGFNEFVGMASDFWMSAIINMAISQHYLVILFVRAYYHLLKTEVRQAIHESQ *F MLSEIYPRRAAFMTKCCYLADRIDNIAKLQNQLQSIVTQLNQVFGIQGIMVYGGYYIFSVATTYITYSLAINGI *F EELHLSVRAAALVFSWFLFYYTSAILNLFVMLKLFDDHKEMERILEERTLFTSALDVRLEQSVSFYPTITELK *F YRDLVLSQFESIQLQLIRNPLKIEVLDIFTITRSSSAAMIGSIITNSIFLIQYDMEYF *F GrLU2=Gr28A3 *F MWLLRRSVGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINIIIRMA *F IYMVNSFYGAVNRDTLMSNFFLTDISNVIDALQKINGMLGIFAILLISLLNRKELLKLLATFDR *F LETEAFPRVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLIC *F EAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVEFVTFFSCQMILYLIA *F IISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGLFNIDRTL *F YFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F GrLU3=Gr64A1 *F MKGPNLNFRKTPSKDNGVKQVESLARPETPPPKFVEDSNLEFNVLASEKLPNYTNLDLFHRA *F VFPFMFLAQCVAIMPLVGIRESNPRRVRFAYKSIPMFVTLIFMIATSILFLSMFTHLLKIGITA *F KNFVGLVFFGCVLSAYVVFIRLAKKWPAVVRIWTRTEIPFTKPPYEIPKRNLSRRVQLAALAI *F IGLSLGEHALYQVSAILSYTRRIQMCANITTVPSFNNYMQTNYDYVFQLLPYSPIIAVLILATC *F TFVWNYMDLFIMMISKGLSYRFEQITTRIRKLEHEEVCESVFIQIREHYVKMCELLEFVDSAM *F SSLILLSCVNNLYFVCYQLLNVFNKLRWPINYIYFWYSLLYLIGRTAFVFLTAADINEESKRGLGVLRR *F VSSRSWCVEVERLIFQMTTQTVALSGKKFYFLTRRLLFGMAGTIVTYELVLLQFDEPNRRKGLQP *F GrLU4 *F IYILSLYIFFQFISNVSLIVVLKLFRDI *F GrLU5 *F BACR28P1-T7 388-734 *F Could not find reading frame *F GrLU6 *F BACR06103-T7 *F Could not locate BAC clone *F GrLU7=Gr5A1 *F MRQLKGRNRCNRAVRHLKVQGKMWLKNLKSGLEQIRESQVRGTRKNFLHDGSFHEAVAPV *F LAVAQCFCLMPVCGISAPTYRGLSFNRRSWRFWYSSLYLCSTSVDLAFSIRRVAHSVLDVR *F SVEPIVFHVSILIASWQFLNLAQLWPGLMRHWAAVERRLPGYTCCLQRARPARRLKLVAFV *F LLVVSLMEHLLSIISVVYYDFCPRRSDPVESYLLGASAQLFEVFPYSNWLAWLGKIQNVLLTF *F GWSYMDIFLMMLGMGLSEMLARLNRSLEQQVRQPMPEAYWTWSRTLYRSIVELIREVDDA *F VSGIMLISFGSNLYFICLQLLKSINTMPSSAHAVYFYFSLLFLLSRSTAVLLFVSAINDQAREP *F LRLLRLVPLKGYHPEVFRFAAELASDQVALTGLKFFNVTRKLFLAMAGTVATYELVLIQFHEDK *F KTWDCSPFNLD # *U FBrf0137429 *a Millburn *b G. *t 2001.4.25 *T personal communication to FlyBase *u FlyBase error report for CG13788 on Wed Apr 25 08:41:27 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 25 16:41:35 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 16:41:35 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Apr 2001 08:41:28 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG13788 on Wed Apr 25 08:41:27 2001 *F Content-Length: 829 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG13788 *F Release: 1 *F Gene annotation error *F Gene CG13788 should be split. *F Comments: CG13788 may need splitting in 2. *F http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html *F (being curated as Scott and Axel, 2001.4.25, personal communication to FlyBase) *F describe 2 gustatory receptor (Gr) genes: 'Gr28A4' and 'GrLU2=Gr28A3' *F Gr28A4 sequence matches the N terminal portion of CG13788. *F GrLU2=Gr28A3 sequences matches the C terminal portion of CG13788, *F suggesting either that the CG13788 annotation needs splitting in two, *F or that Gr28A4 and GrLU2=Gr28A3 need merging. *F alignments of the 2 Gr genes to CG13788 follow in e-mail to flybase-updates *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From gm119@gen.cam.ac.uk Wed Apr 25 16:45:01 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 16:45:01 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: CG13788 alignments (for FlyBase error report) *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 25 Apr 2001 16:44:59 \+0100 *F Content-Length: 5069 *F here are alignments *F Query= Gr28A4 , 415 bases, C04ED3E5 checksum. *F (415 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG13788|FBan0013788|CT33277|FBan0013788 last_updated:000321 1863 1.1e-193 1 *F CG13787|FBan0013787|CT33276|FBan0013787 last_updated:000321 206 1.5e-15 1 *F CG10346|FBan0010346|CT29062|FBan0010346 last_updated:000321 70 0.992 1 *F CG5755|FBan0005755|CT18089|FBan0005755 last_updated:000321 68 0.994 1 *F >CG13788|FBan0013788|CT33277|FBan0013788 last_updated:000321 *F Length = 1227 *F Score = 1863 (655.8 bits), Expect = 1.1e-193, P = 1.1e-193 *F Identities = 368/415 (88%), Positives = 368/415 (88%) *F Query: 1 MIRCGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSW 60 *F MIRCGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSW *F Sbjct: 1 MIRCGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSW 60 *F Query: 61 YGFLNAIFRWLLLAYCYSYINLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPL 120 *F YGFLNAIFRWLLLAYCYSYINLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPL *F Sbjct: 61 YGFLNAIFRWLLLAYCYSYINLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPL 120 *F Query: 121 LLRKYFLKSVKNMVQVDTQLERLRSPVNFNXXXXXXXXXXXXXXXXXXXXXXXXXXXXAK 180 *F LLRKYFLKSVKNMVQVDTQLERLRSPVNFN AK *F Sbjct: 121 LLRKYFLKSVKNMVQVDTQLERLRSPVNFNTVVGQVVLVILAVVLLDTVLLTTGLVCLAK 180 *F Query: 181 MEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRITCLHKFDFATMSALRRVRKYFI 240 *F MEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRITCLHKFDFATMSALRRVRKYFI *F Sbjct: 181 MEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRITCLHKFDFATMSALRRVRKYFI 240 *F Query: 241 SSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNXXXXXXXXGFCYISSL 300 *F SSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFN GFCYISSL *F Sbjct: 241 SSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNIFIYICLCGFCYISSL 300 *F Query: 301 RQGESIVGYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFS 360 *F RQGESIVGYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFS *F Sbjct: 301 RQGESIVGYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFS 360 *F Query: 361 NIGVRVKYSRIFRYSXXXXXXXXXXXGVYFVGVFRLLVSLDVTPSFCVCMTFFLQ 415 *F NIGVRVKYSRIFRYS GVYFVGVFRLLVSLDVTPSFCVCMTFFLQ *F Sbjct: 361 NIGVRVKYSRIFRYSLLVLIFKLLILGVYFVGVFRLLVSLDVTPSFCVCMTFFLQ 415 *F Query= GrLU2=Gr28A3 , 365 bases, 9132649A checksum. *F (365 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG13788|FBan0013788|CT33277|FBan0013788 last_updated:000321 1687 4.8e-175 1 *F >CG13788|FBan0013788|CT33277|FBan0013788 last_updated:000321 *F Length = 1227 *F Score = 1687 (593.9 bits), Expect = 4.8e-175, P = 4.8e-175 *F Identities = 337/365 (92%), Positives = 337/365 (92%) *F Query: 1 MWLLRRSVGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINII 60 *F MWLLRRSVGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINII *F Sbjct: 863 MWLLRRSVGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINII 922 *F Query: 61 IRMAIYMVNSFYGAVNRDTLMSNFFLTDISNVIDALQKINGMLGIFAIXXXXXXXXXXXX 120 *F IRMAIYMVNSFYGAVNRDTLMSNFFLTDISNVIDALQKINGMLGIFAI *F Sbjct: 923 IRMAIYMVNSFYGAVNRDTLMSNFFLTDISNVIDALQKINGMLGIFAILLISLLNRKELL 982 *F Query: 121 XXXATFDRLETEAFPRVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEII 180 *F ATFDRLETEAFPRVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEII *F Sbjct: 983 KLLATFDRLETEAFPRVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEII 1042 *F Query: 181 QESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTV 240 *F QESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTV *F Sbjct: 1043 QESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTV 1102 *F Query: 241 EFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQ 300 *F EFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQ *F Sbjct: 1103 EFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQ 1162 *F Query: 301 LMHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTXXXXXXXXXXXXXCCETFNNM 360 *F LMHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFT CCETFNNM *F Sbjct: 1163 LMHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNM 1222 *F Query: 361 TNHTL 365 *F TNHTL *F Sbjct: 1223 TNHTL 1227 # *U FBrf0137430 *a Millburn *b G. *t 2001.4.25 *T personal communication to FlyBase *u FlyBase error report for CG15137 on Wed Apr 25 09:22:53 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 25 17:22:56 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 17:22:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Apr 2001 09:22:53 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG15137 on Wed Apr 25 09:22:53 2001 *F Content-Length: 786 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG15137 *F Release: 1 *F Gene annotation error *F Gene CG15137 should be split. *F Comments: http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html *F (being curated as Scott and Axel, 2001.4.25, personal communication to FlyBase) *F describes 2 gustatory receptor (Gr) genes: Gr36B.2 and Gr36B.3, both of which *F overlap somewhat with CG15137, suggesting that CG15137 may need splitting into *F 2 genes, or Gr36B.2 and Gr36B.3 may need merging (the alignment *F of Gr36B.2 with CG15137 is not that great, so I'm not sure what needs *F to happen). *F alignments of the 2 Gr genes to CG15137 follow in e-mail to flybase-updates *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From gm119@gen.cam.ac.uk Wed Apr 25 17:24:20 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 17:24:20 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: CG15137 alignments (for FlyBase error report) *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 25 Apr 2001 17:24:14 \+0100 *F Content-Length: 4658 *F here are alignments *F BLASTP of GR against predicted proteins: *F Query= Gr36B2 , 390 bases, A460EF58 checksum. *F (390 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 440 2.0e-50 2 *F >CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 *F Length = 361 *F Score = 440 (154.9 bits), Expect = 2.0e-50, Sum P(2) = 2.0e-50 *F Identities = 82/201 (40%), Positives = 132/201 (65%) *F Query: 90 LITVLNRWLQRGQMMQLVKDVIRLYMINPQLKSMIRWGILLKAFISFAIELLQVTLSVDA 149 *F L T++ RW QR \+MM L V+R+Y+ PQ++ M RWGIL K \+ LQ+ \+ \+ A *F Sbjct: 131 LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMVLSA 190 *F Query: 150 LDRQGTAEMMGLLVKLCVSFIMNLAISQHFLVILLIRAQYRIMNAKLRMVIEESRRLSFL 209 *F \+ \+ \+GL \++ \+ I+N+A+ Q \+++L \+R Q++++N \+LR VI+E++ L *F Sbjct: 191 MGSVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKDLLLS 250 *F Query: 210 QLRNGAFMTRCCYLSDQLEDIGEVQSQLQSMVGQLDEVFGMQGLMAYSEYYLSIVGTSYM 269 *F G FMT+CC L+DQ+E+I \+QSQLQ+++ Q++EVFG+QG M Y YYLS VGT Y+ *F Sbjct: 251 PRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVGTCYL 310 *F Query: 270 SYSIYKYGPHNLKLSAKTSII 290 *F \+YSI K+G NL \++ T I+ *F Sbjct: 311 AYSILKHGYENLSMTLSTVIL 331 *F Score = 281 (98.9 bits), Expect = 4.7e-26, P = 4.7e-26 *F Identities = 60/81 (74%), Positives = 66/81 (81%) *F Query: 260 YLSIVGTSYMSYSIYKYGPHNLKLSAKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF 319 *F \+L \+ \++M+ Y L+ AKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF *F Sbjct: 17 FLQLRNGAFMTRCCYL--SDQLEDIAKTSIIVCILITLFYLDALVNCNNMLRVLDHHKDF 74 *F Query: 320 LGLLEERTVFASSLDIRLEES 340 *F LGLLEERTVFASSLDIRLEES *F Sbjct: 75 LGLLEERTVFASSLDIRLEES 95 *F Score = 200 (70.4 bits), Expect = 5.7e-15, P = 5.7e-15 *F Identities = 42/76 (55%), Positives = 53/76 (69%) *F Query: 192 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIGEVQSQLQSMVG--QLDEVFG 249 *F MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDI \+ \+ \++ LD \+ *F Sbjct: 1 MNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIAKTSIIVCILITLFYLDALVN 60 *F Query: 250 MQGLMAYSEYYLSIVG 265 *F \++ \+++ \+G *F Sbjct: 61 CNNMLRVLDHHKDFLG 76 *F Score = 88 (31.0 bits), Expect = 2.0e-50, Sum P(2) = 2.0e-50 *F Identities = 19/34 (55%), Positives = 23/34 (67%) *F Query: 1 MVDWVVLLLKAVHIYCYLIGLSNFEFDCRTGRVF 34 *F \++D LL AV+ Y IGLSNFEFD TGR+F *F Sbjct: 99 IMDLESFLLGAVYYYGLFIGLSNFEFDWNTGRLF 132 *F Query= Gr36B3 , 344 bases, CF996511 checksum. *F (344 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 1053 7.3e-108 1 *F >CG15137|FBan0015137|CT35034|FBan0015137 last_updated:000321 *F Length = 361 *F Score = 1053 (370.7 bits), Expect = 7.3e-108, P = 7.3e-108 *F Identities = 212/228 (92%), Positives = 215/228 (94%) *F Query: 89 TG-LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV 147 *F TG LFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV *F Sbjct: 128 TGRLFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMV 187 *F Query: 148 LSAMGSRVDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD 207 *F LSAMGS VDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD *F Sbjct: 188 LSAMGS-VDSQFYLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKD 246 *F Query: 208 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG 267 *F LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG *F Sbjct: 247 LLLSPRHQGVFMTKCCSLADQIENIARIQSQLQTIMNQMEEVFGIQGAMTYGGYYLSSVG 306 *F Query: 268 TCYLAYSILKHGYENLSMTLSTVILAYSW-----CFFYYLDGMLNLSVM 311 *F TCYLAYSILKHGYENLSMTLSTVILAYSW C+ Y G \+L+ M *F Sbjct: 307 TCYLAYSILKHGYENLSMTLSTVILAYSWMTTGKCYKYSGSGQYSLAWM 355 # *U FBrf0137431 *a Millburn *b G. *t 2001.4.25 *T personal communication to FlyBase *u FlyBase error report for CG14988 on Wed Apr 25 09:49:07 2001. *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 25 17:49:10 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 17:49:10 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 25 Apr 2001 09:49:07 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG14988 on Wed Apr 25 09:49:07 2001 *F Content-Length: 811 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG14988 *F Release: 1 *F Gene annotation error *F Gene CG14988 should be split. *F Comments: CG14988 may need splitting in 2. *F http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html *F (being curated as Scott and Axel, 2001.4.25, personal communication to FlyBase) *F describe 2 gustatory receptor (Gr) genes: 'Gr64A2' and 'Gr64A3' *F Gr64A2 sequence matches the N terminal portion of CG14988. *F Gr64A3 sequences matches the C terminal portion of CG14988, *F suggesting either that the CG14988 annotation needs splitting in two, *F or that Gr64A2 and Gr64A3 need merging. *F alignments of the 2 Gr genes to CG14988 follow in e-mail to flybase-updates *F Gillian *F Browser: Mozilla/4.7 en (X11; I; SunOS 5.6 sun4u) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs *F From gm119@gen.cam.ac.uk Wed Apr 25 17:50:06 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 25 Apr 2001 17:50:06 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: CG14988 alignments (for FlyBase error report) *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Wed, 25 Apr 2001 17:50:04 \+0100 *F Content-Length: 5523 *F here are alignments *F BLASTP of GR against predicted proteins: *F Query= Gr64A2 , 367 bases, E86E1572 checksum. *F (367 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 1822 2.4e-189 1 *F CG15779|FBan0015779|CT36038|FBan0015779 last_updated:000321 666 7.5e-67 1 *F CG14986|FBan0014986|CT34837|FBan0014986 last_updated:000321 466 2.3e-44 1 *F CG14987|FBan0014987|CT34838|FBan0014987 last_updated:000321 129 2.3e-16 2 *F CG13888|FBan0013888|CT33421|FBan0013888 last_updated:000321 152 9.0e-10 1 *F >CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 *F Length = 817 *F Score = 1822 (641.4 bits), Expect = 2.4e-189, P = 2.4e-189 *F Identities = 353/367 (96%), Positives = 353/367 (96%) *F Query: 1 MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFY 60 *F MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFY *F Sbjct: 1 MPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFY 60 *F Query: 61 LSIFLISLSFFQLARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLV 120 *F LSIFLISLSFFQLARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLV *F Sbjct: 61 LSIFLISLSFFQLARKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLV 120 *F Query: 121 EHIMSMLSMGYYVNSCPRWPDRPIDXXXXXXXXXXXXXXDYTRFLGIVGKVVNVLSTFAW 180 *F EHIMSMLSMGYYVNSCPRWPDRPID DYTRFLGIVGKVVNVLSTFAW *F Sbjct: 121 EHIMSMLSMGYYVNSCPRWPDRPIDSFLYLSFSSVFYFVDYTRFLGIVGKVVNVLSTFAW 180 *F Query: 181 NFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAIS 240 *F NFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAIS *F Sbjct: 181 NFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAIS 240 *F Query: 241 TITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESK 300 *F TITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESK *F Sbjct: 241 TITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESK 300 *F Query: 301 RPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILL 360 *F RPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILL *F Sbjct: 301 RPLVIFRLVPREYWCDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILL 360 *F Query: 361 QFNGEEK 367 *F QFNGEEK *F Sbjct: 361 QFNGEEK 367 *F BLASTP of GR against predicted proteins: *F Query= Gr64A3 , 409 bases, EAD6D3F1 checksum. *F (409 letters) *F Database: aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 2066 3.3e-215 1 *F CG15779|FBan0015779|CT36038|FBan0015779 last_updated:000321 904 4.5e-92 1 *F CG14986|FBan0014986|CT34837|FBan0014986 last_updated:000321 578 2.4e-56 1 *F CG14987|FBan0014987|CT34838|FBan0014987 last_updated:000321 187 1.1e-25 2 *F CG13888|FBan0013888|CT33421|FBan0013888 last_updated:000321 194 1.2e-14 1 *F >CG14988|FBan0014988|CT34839|FBan0014988 last_updated:000321 *F Length = 817 *F Score = 2066 (727.3 bits), Expect = 3.3e-215, P = 3.3e-215 *F Identities = 397/409 (97%), Positives = 397/409 (97%) *F Query: 1 MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSL 60 *F MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSL *F Sbjct: 409 MELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSL 468 *F Query: 61 LFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGSXXXXXXXXXXXXRQWPQLMMYWHTV 120 *F LFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGS RQWPQLMMYWHTV *F Sbjct: 469 LFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGSVLLVLIVALNLARQWPQLMMYWHTV 528 *F Query: 121 EKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYF 180 *F EKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYF *F Sbjct: 529 EKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYF 588 *F Query: 181 LRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNF 240 *F LRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNF *F Sbjct: 589 LRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNF 648 *F Query: 241 KGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSV 300 *F KGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSV *F Sbjct: 649 KGMNMAPSYWSERRIQYRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSV 708 *F Query: 301 AHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD 360 *F AHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD *F Sbjct: 709 AHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTEEVISD 768 *F Query: 361 EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS 409 *F EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS *F Sbjct: 769 EVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS 817 # *U FBrf0137441 *a Hewes *b R.S. *c P.H. *d Taghert *t 2001.7.31 *T personal communication to FlyBase *u *F [FlyBase curator comment: sequence of Drosophila melanogaster peptide GPCRs, *F taken from web page http://thalamus.wustl.edu/flyGPCR/sequences.html on *F 31.7.2001.] *F Sequences of the candidate Drosophila melanogaster peptide GPCRs (TM1-TM7 *F only; ectodomains for CG4187 and CG5042 listed at the end) *F >BACR25B3.3 *F ARRTRTLEIVGLCLSLFALIVSLLIFCTFRSLRNNRTKIHKNLFVAMVLQVIIRLTLYLDQFRRNTMPYLCEASYVLLE *F YARTAMFMWMFIEGLYLHNMVTVAVFQGSFPLKFFSRLGWCVPILMTTVWARCTVMYMDTSLGECLWNYNLTPYYWILE *F GPRLAVILLNFCFLVNIIRVLVMKLRKAVRAAIVLLPLLGITNLLHQLAPLKTATNFAVWSYGTHFLTSFQGFFIALIY *F CFLNGEV *F >BACR48G21.1 *F GNFSMVYVIATNRSLRSPTNLIIANMAVADLLTLAICPAMFMVNDFYQNYQLGCVGCKLEGFLVVVFLITAVLNLSVVS *F YDRLTAIVLPMETRLTIRGVQIVVVCTWVSGILLASPLAFYRSYRVRVWKNFTERYCKENTSVLPKYWYVLITILVWLP *F LGIMLICYIAIFYKLDRYEKRYKRSVAKTLFIVVVVFAALRLPFTILVVLREKYFGEDVSVSSGMQLFWYISQYLMFLN *F AAVNPLIY *F >CG10001 *F GNLLVILVVVFNNNMRSTTNLMIVNLAAADLMFVILCIPFTATDYMVYYWPYGRFWCRSVQYLIVVTAFASIYTLVLMS *F IDRFLAVVHPIRSRMMRTENITLIAIVTLWIVVLVVSVPVAFTHDVVVDYDAKKNITYGMCTFTTNDFLGPRTYQVTFF *F ISSYLLPLMIISGLYMRMIMRLWRQGTGVRMSKESQRGRKRVTRLVVVVVIAFASLWLPVQLILLLKSLDVIETNTLTK *F LVIQVTAQTLAYSSSCINPLLY *F >CG10626 *F GNTLVIWVVATTRQMRTVTNMYIANLAFADVIIGLFCIPFQFQAALLQSWNLPWFMCSFCPFVQALSVNVSVFTLTAIA *F IDRHRAIINPLRARPTKFVSKFIIGGIWMLALLFAVPFAIAFRVEELTERFRENNETYNVTRPFCMNKNLSDDQLQSFR *F YTLVFVQYLVPFCVISFVYIQMAVRLWGTRAPGNAKKVIKMLIIVVIIFGLCWLPLQLYNILYVTIPEINDYHFISIVW *F FCCDWLAMSNSCYNPFIY *F >CG10698 *F GNLLTIWNIYKTRISRRNSRHTWSAIYSLMFHLSIADVLVTWFCIIGEAAWCYTVQWLANELTCKLVKLFQMFSLYLST *F YVLVLIGVDRWIAVKYPMKSLNMAKRCHRLLGGTYILSLVLSLPQFFIFHVARGPFVEEFYQCVTHGFYTADWQEQMYA *F TFTLVFTFLLPLCILFGTYMSTFRTISSSEKMFQGSKLANYSTAKLPTQTNRQRLIHKAKMKSLRISVVIIIAFLICWT *F PYYVMMIMFMFLNPDKRLGDDLQDAAIFFFGMSNSLVNPLIY *F >CG10823 *F GNSFVIAVVLRAPRMRTVTNYFIVNLAIADILVIVFCLPATLIGNIFVPWMLGWLMCKFVPYIQGVSVAASVYSLIAVS *F LDRFIAIWWPLKQMTKRRARIMIIGIWVIALVTTIPWLLFFDLVPAEEVFSDALVSAYSQPQFLCQEVWPPGTDGNLYF *F LLANLVACYLLPMSLITLCYVLIWIKVSTRSIPGESKDAQMDRMQQKSKVKVIKMLVAVVILFVLSWLPLYVIFARIKF *F GSDISQEEFEILKKVMPVAQWLGSSNSCINPILY *F >CG1147 *F GNTLVVIAVIRKPIMRTARNLFILNLAISDLLLCLVTMPLTLMEILSKYWPYGSCSILCKTIAMLQALCIFVSTISITA *F IAFDRYQVIVYPTRDSLQFVGAVTILAGIWALALLLASPLFVYKELINTDTPALLQQIGLQDTIPYCIEDWPSRNGRFY *F YSIFSLCVQYLVPILIVSVAYFGIYNKLKSRITVVAVQASSAQRKVERGRRMKRTNCLLISIAIIFGVSWLPLNFFNLY *F ADMERSPVTQSMLVRYAICHMIGMSSACSNPLLY *F >CG12370 *F VELPAIIYAGGYFLSFATLVVALIIFLSFKDLRCLRNTIHANLFLTYITSALLWILTLFLQVITSQAGCITLVIMFQYF *F YLTNFFWMFVEGLYLYTLVVQTFSSDNISFIIYALIGWGCPAVCILVWSIAKAFAPHLENEHFNGLEIDCAWMRESHID *F WIFKVPASLALLVNLVFLIRIMWVLITKLRKASKALLVLIPLFGITYLLVLTGPEQGISRNLFEAIRAFLISTQGFFVA *F LFYCFLNSEV *F >CG12610 *F LNVIIVVYIMYHRLYKDVTHAFIINLALCHFVQCALVLPVSLMVMLIQNWIFGQFLCFFLPMLQDIPLHVAMISHILIA *F WDRMRWLNDPLKGRLPGFVCCCATWLTGMVIALPYPIYTIYVELGDYMPQLSGIGLCVVNLMDDMQEYTRGLFLLMYCG *F PAILLSYLYIRTSQELRPPDGPFAVMMYEHRADLRMRQRSSTSSVEPRHLSGGGVAGLSNGGGNSARSYDLYSAELDVH *F REKRKQRNFGSMAATQVVCMCPLMILRFARLSLEETYENAKHFDFTYLMFVWVAFLPTVIFPCIY *F >CG13229 *F ANILNIMVLTRKEMAKTPINNILKWLAVADMFVMLEYIPYTSYQYIYMGPGEKDLSYTWAVCLLVHMHFTQILHTISIG *F LTVTLAVWRYVAIRHPNGGCANFLLAHSREAILLPFILSPILCLPTYFVFQVRETYDVDKVNSEAMYHVYFDKDSVLYR *F FNFWIHSVLIKLLPCGILIVISAVLMHVLCEASRRRLKLRDYNNPAKYAIQLNLNETKSKKPPRCDRRNDRTTLLLVAV *F LVLFLITEFPQGLLGLLSGVMEKCFFAHCYPPFGELMDLLALINAAVGFVLY *F >CG13575 *F GNLSTLYVNSRRKLRPFFRACLISLACSDLVSSIFCTVSYMAQFQAQYLQLWTIGGFMCKFVPFITTTSVLSGSLTLVA *F IALDRYLAVMRPVLGFWSPDKRFSTLSMLLIWACSIGSSGPLLGIYDYRKIYCLAGDHDVGLYYVILFTLIFLPCIVSF *F LWLNAVIARQLWLRRHARHRKMVVVVLLMMAVFICLRLPAWVFLIMRLYGSYSEPIDWLLYFSFGILNLFSCALNPIFY *F >CG13702 *F GNTLVIYVVMRFSKMQTVTNIYILNLAIADECFLIGIPFLLYTMQVGNWPFGNYMCKAYMVSTSITSFTSSIFLLIMSA *F DRYIAVCHPISSPRYRTPFVSKLVSAFAWMTSVLLMLPVILFASTVQSSNGNVSCNIEWPDTQNSHTDSTFILYSLVLG *F FATPLTFILVFYCLVIRKLHTVGPKHKSKEKKRSHRKVTKLVLTVISAYIFCWLPHWISQVTNCIHYSLKTFIFLAVFL *F ACGCLSYSNSAMNPILY *F >CG13803 *F ANTLNIIVLTRREMRSPTNAILTGLAVADLAVMLEYIPYTVHDYILSVRLPREEQLSYSWACFIKFHSVFPQVLHTISI *F WLTVTLAVWRYIAVSYPQRNRIWCGMRTTLITIATAYVVCVLVVSPWLYLVTAIAKFLETLDANGKTIASVPLSQYILD *F YNRQDEVTMQVMSSTTPDVSWAIPITTSSSLGERNVTVYKLYHSALALRDRQFRNATFLIYSVLIKLIPCFALTILSVR *F LIGALLEAKRRRKILACHAANDMQPIVNGKVVIPTQPKSCKLLEKEKQTDRTTRMLLAVLLLFLVTEFPQGIMGLLNVL *F LGDAFFLQCYLKLSDLMDILALINSSINFILY *F >CG13995 *F GNSTLVLTLCSASSVRLRNPLLLAVCIADLLVTGISAPVTLLNLAMNRRTRSLPLVLCKVIHYVQVMPVSASTISFFML *F SLDRYATVKHPRLAQLRQRRYLHVSLALLSWLASAAISTPFLFAYKIIAKSMVVKGGGAANTTPNPVSISCTSDLGANA *F MFMSFIIFHTIAVFVLPGIGVLLNHYGVRRKLCALSLTARAAHGELPLPIPILRRQTHMVIVTGCPNAQQAACGGGTTA *F DDTSNGNGTGTGGGPMAVSPGDIQLHTLQPRQPGSAGSALEPGSYRSSNPISPRAMREIRAHSQRQRINRAGRGPATPG *F IPLPQTSTLRSRRHLANMLIASAVIFIACWAPHVFCIFYKNFGNNQQCSQTSVYFSLLLGYFYSAISPVIY *F >CG14003 *F GNLLVILVVTLSRRLRSITNFFLANLAFADFCVGLFCVMQNLSIYLIESWVFGEFLCRMYQFVHSLSYTASIFILVVIC *F MERYFAIVHPITCKQILTAARLRMVIVTVWITSAVYSTPKFVFSKTIKNIHTQDGQEEEICVLDREMFNSKLLDMINFV *F LLYVMPLLVMTVLYSKIAIALWRSSRGLTPHVVQHQHQQPQQPSCQDIGMGMHNSMYHHHPHHHHHHHQHHQLQSAASS *F AGVVGVGLGGGGGGGPGPSLASGGSSTTSLRKQSSKYEKRGVSITESQLAVVKTLSPVAVTPALSPGQVARCSEQVSIH *F IKAIAGPCHKLGHRCDTWSVEWWSEVTTGTGRNCKCEAKVSLEADRPIVSACRKTSFYHHGHAHHQRAGNASVGGGSGG *F AGAGATHMSHSSSNVLRARRGVVRMLIIFVLTFALCNLPYHARKMWQYWSRSYRGDSNFNALLTPLTFLVTYFNSGVNP *F LLY *F >CG14484 *F GNGTLIVVFLSVRQMRNVPNTYILSLALADLLVIITTVPLASTVYTVEYWPYGSFLCSLSEFMKDVSIGVSVFTLTALS *F GDRYFAIVDPLRKFHAHGGGRRATRMTLATAVSIWLLAILCGLPALIGSNLKHLGINEKSIVICYPYPEEWGINYAKSM *F VLLHFLVYYAIPLVVIAVFYVLIALHLMYSASVPGEIQGAVRQVRARRKVAVTVLAFVVIFGICFLPYHVFFLWFYFWP *F TAQDDYNAFWHVLRIVAYCMSFANSCANPVAL *F >CG14575 *F GNLLVCIVIIRHSAMMTATNYYLFSLAVSDLLYLLFVFLYWHQYPDLFGMPFCKIRAFISEACTYVSVFTIVAFSMERF *F LAICHPLHLYAMVGFKRAIRIITALWIVSFISAIPFGLLSDIQYLNYPFHSRIEESAFCSMSPKIVNEIPVFEVSFCIF *F FVIPMILIILLYGRMGAKIRSRTNQKLGKYLAFIDPKRSDFRDVFQVIAAVVITFFVCWFPFHLQRLIFLYAKNMDNYL *F DINEALFSIAGFAYYVSCTVNPIVY *F >CG14593 *F GNGTLVIIFFRHRSMRNIPNTYILSLALADLLVILVCVPVATIVYTQESWPFERNMCRISEFFKDISIGVSVFTLTALS *F GERYCAIVNPLRKLQTKPLTVFTAVMIWILAILLGMPSVLFSDIKSYPVFTATGNMTIEVCSPFRDPEYAKFMVAGKAL *F VYYLLPLSIIGALYIMMAKRLHMSARNMPGEQQSMQSRTQARARLHVARMVVAFVVVFFICFFPYHVFELWYHFYPTAE *F EDFDEFWNVLRIVGFCTSFLNSCVNPVAL *F >CG16726 *F GNILSVFVFFRTKLRKLSSSFYLAALAVSDTCFLAGLFAQWLNFLNVDIYNQNYFCQFFTFFSYLASFCSVWFVVAFTV *F ERFIAVIYPLKRQTMCTVRRAKIVLFCLTLVGCLHCLPYIVIAKPVFMPKLLALFNYWDTIVVYAVPFTTIAVLNTCTG *F CTVWKFAVQNSSQLKVTKMLLIVSTVFVCLNLPSCLLRIEAYWETESARNQNSTIALQYIFHAFFITNFGINFVLY *F >CG17415 *F RHTVNLISEVGYGTSLLAILLSLAILGYFNNSIRIFVFRLNRSLKCARITLHMNLFASFAANNSLWLVWYLLVMPNSEL *F LHQSPMRCVALHITLHYFLLSNYSWMLCEGFYLHTVLVAAFISEKRLVKWLIAFGWGSPAIVIFVYSMARGLGGTPEDN *F RHCWMNQTNYQNILMVPVCISMFLNLLFLCNIVRVVLLKLNAPASIQGSCGPSRTVLQAFRATLLLVPLLGLQYILTPF *F RPAPKHPWENTYEIISAFTASFQGLCVAILFCFCNGEV *F >CG2114 *F GNIISMIILSRPQMRSSINYLLTGLARCDTVLIITSILLFGIPSIYPYTGHFFGYYNYVYPFISPAVFPIGMIAQTASI *F YMTFTVTLERYVAVCHPLKARALCTYGRAKIYFIVCVCFSLAYNMPRFWEVLTVTYPEPGKDVILHCVRPSRLRRSETY *F INIYIHWCYLIVNYIIPFLTLAILNCLIYRQVKRANRERQRLSRSEKREIGLATMLLCVVIVFFMLNFLPLVLNISEAF *F YSTIDHKITKISNLLITINSSVNFLIY *F >CG4187 *F GNLLVLLGRYFYKSRSNVEHSLYLRHLAASDFLMGIYLTLIACADISFRGEYIKYEETWRHSGVCAFAGFLSTFSCQSS *F TLLLTLVTWDRLMSVTRPLKPRDTEKVRIVLRLLLLWGISFGLAAAPLLPNPYFGSHFYGNNGVCLSLHIHDPYAKGWE *F YSALLFILVNTLSLIFILFSYIRMLQAIRDSGGGMRSTHSGRENVVATRFAIIVTTDCACWLPIIVVKLAALSGCEISP *F DLYAWLAVLVLPVNSALNPVLY *F >CG4395 *F RQFINELYVKGYALSLLALLISIIIFLGFKSLRCTRIRIHVHLFASLACTCVAWILWYRLVVEINITNFQLWCIGLHLV *F VHYFMLVNYFWMFCEGLHLHLVLVVVALRLRQVEMSGMSVQILTPLLLSIRQVFVKDTIVMRCCWITDSLYLWIFSYAI *F AQLYAFYPTVFLINVLRVIVRKLHPQSAQPAPLAIRKAVRATIILVPLFGLQHFLLPYRPDAGTQLDHFYQMLSVVLVS *F LQGFVVSFLFCFANHDV *F >CG5042 *F GNVLVLWGRFIYRDENVAVTMVIRNLALADMLMGFYLVTIGVQDYRYRNEYYKVVLDWITSWQCTLIGTLAVSSSEVSM *F LILAFMSLERFLLIADPFRGHRSIGNRVMWLALICIWITGVGLAVAPVLLWRTSTLPYYGSYSGTCFPLHIHEAFPMGW *F LYSAFVFLGVNLLLLVMIAMLYTALLISIWRTRSATPLTLLDCEFAVRFFFIVLTDFLCWVPIIVMKIWVFFNYNISDD *F IYAWLVVFVLPLNSAVNPLLY *F >CG5811/107368 *F GNGTVCYIVYSTPRMRTVTNYFIASLAIGDILMSFFCEPSSFISLFILNYWPFGLALCHFVNYSQAVSVLVSAYTLVAI *F SIDRYIAIMWPLKPRITKRYATFIIAGVWFIALATALPIPIVSGLDIPMSPWHTKCEKYICREMWPSRSQEYYYTLSLF *F ALQFVVPLGVLIFTYARITIRVWAKRPPGEAETNRDQRMARSKRKMVKMMLTVVIVFTCCWLPFNILQLLLNDEEFAHW *F DPLPYVWFAFHWLAMSHCCYNPIIY *F >CG5911spliceA *F GNVMVPIVIVKTKDMRNSTNIFLTNLSIADLLVLLVCTPTVLVEVNTRPETWVLGHEMCKAVPFVELTVAHASVLTILA *F ISFERYYAICEPLKAGYVCTKGRAILICVLAWGIAALFTSPILWVAEYKLAEYIDGSSVAVCLTQAISDWTLAFFLMTI *F SVFFVVPFVTLVVLYGIIARNLVSNRAAMLRARPTKPELSLKARKQVVLMLGAVVLSFFVCLLPFRVLTLWIILSTDQT *F LHDLGLVRYYSLLYFCRIMLYLNSAMNPILY *F >CG5911spliceB *F GNVMVPIVIVKTKDMRNSTNIFLTNLSIADLLVLLVCTPTVLVEVNTRPETWVLGHEMCKAVPFVELTVAHASVLTILA *F ISFERYYAICEPLKAGYVCTKGRAILICVLAWGIAALFTSPIIAISTYSVEPYGDGTDAPVCTTAADGFWSIFYFVGCI *F TVFFFLPFGILVLLYAAIAYKLLRPNNAFHRPTSPQPQQPSGGATSGSSQVPSTKGNSHQQSNGMRKHRKQVIFMLVAV *F VSSFFVCLLPFRAFTLWVILASAEDVEGLGIAGYYNLLYFSRFMLYLNSAMNPILY *F >CG5936 *F GNVLNLVVLTRRNMRGTAYIYMRAYSTAALLAIVFAIPFGIRMLVHKDRGQWEEFGPAFYTAHLELYLGNGCLGQNIIP *F FALLGVGVMMLLVLTIERYVSVCHPGFARPVMGPPGVVVFLTCLATVIVYLPSIFRGELIKCILGSSDVYVYLRRDNTI *F YQQTIFYRVYKIMLEVIFKLVPTLVIGGLNMRIMMVYRRTCERRRKMVLSRPHAQGHGHGHGHGHGHGHGHAHGHGYLK *F DDDPRKFAEERRLFLLLGSTSILFLVCVSPMAILHMTIASEVYPSFPFQVFRASANLLELINYSLTFYIY *F >CG6111 *F GNSAVLFVMFINKNRKSRMNYFIKQLALADLCVGLLNVLTDIIWRITISWRAGNLACKAIRFSQVCVTYSSTYVLVAMS *F IDRYDAITHPMNFSKSWKRARHLVAGAWLISALFSLPILVLYEEKLIQGHPQCWIELGSPIAWQVYMSLVSATLFAIPA *F LIISACYAIIVKTIWAKGSIFVPTERAGFGAAPARRASSRGIIPRAKVKTVKMTLTIVFVFIICWSPYIIFDLLQVFGQ *F IPHSQTNIAIATFIQSLAPLNSAANPLIY *F >CG6857 *F GNLLVVLTLVQNRRMRTITNVFLLNLAISDILLGVFCMPVTLVGTLLRHFIFGELLCKLIQFAQGAASVAVSSWTLVAI *F SCERYYAICHPLRSRTWQTINHANKIIAIIWLGSLVCMTPIAAFSQLMPTSRPGLRKCREQWPADSLNYERAYNLFLDL *F ALLVLPLLALSFTYLFITRTLYVSMRNERAMNLESKKRVVKMLFVLVLEFFICWTPLYVINTMTMLLGPTVYEYVGYTS *F ISFLQLLAYSSSCCNPITY *F >CG6881 *F GNLLVISTLVQNRRMRTITNVFLLNLAISDMLLGVLCMPVTLVGTLLRNFIFGEFLCKLFQFSQAASVAVSSWTLVAIS *F CERYYAICHPLRSRSWQTISHAYKIIGFIWLGGILCMTPIAVFSQLIPTSRPGYCKCREFWPDQGYELFYNILLDFLLL *F VLPLLVLCVAYILITRTLYVGMAKDSGRILQQSLPVSATTAGGSAPNPGTSSSSNCILVLTATAVYNGWRRINQYGNDH *F LDNETNGSNCDHHHHDDHGDAGQDLLAQHSRPRCGTSQVGFVSDNRSANPIPYSHFSTIHNPLSRSNEAKTLESKKRVV *F KMLFVLVLEFFICWTPLYVINTMVMLIGPVVYEYVDYTAISFLQLLAYSSSCCNPITY *F >CG6986 *F GNSVSIYVLTRKRMRCTTNIYLTALAITDIAYLTCQLILASLIAFVLYCHAPIHNCFLCFYRTGYISIYIAVCFTIERF *F IAIRYPLKRQTFCTESLAKKNPFYATLYATLYSTLPAVAIFCLLSTLSTAFEHTITIGTRQIDDAYQPCNQTVTYYNHG *F LSELGYDELYSYLWNLFTLLVFVVFPLLLLATFNSILILLVHRSKNLRGDLTNASSIRRTKRKSNSGLKGSVSQENRVT *F ITLIAVVLMFIVCQLPWAIYLIVNQYMEIQIGTQVVAGNVCNLLASLHAASNFFLY *F >CG7285 *F GNTLVIYVVLRFSKMQTVTNIYILNLAVADECFLIGIPFLLYTMRICSWRFGEFMCKAYMVSTSITSFTSSIFLLIMSA *F DRYIAVCHPISSPRYRTLHIAKVVSAIAWSTSAVLMLPVILYASTVEQEDGINYSCNIMWPDAYKKHSGTTFILYTFFL *F GFATPLCFILSFYYLVIRKLRSVGPKPGTKSKEKRRAHRKVTRLVLTVISVYILCWLPHWISQVALHISNPAQRDLSRL *F EILIFLLLGALVYSNSAVNPILY *F >CG7395 *F GNVLVCYVVLRNRAMQTVTNIFITNLALSDILLCVLAVPFTPLYTFMGRWAFGRSLCHLVSFAQGCSIYISTLTLTSIA *F IDRYFVIIYPFHPRMKLSTCIGIIVSIWVIALLATVPYGMYMKMTNELVNGTQTGNEETLVEATLMLNGSFVAQGSGFI *F EAPDSTSATQAYMQVMTAGSTGPEMPYVRVYCEENWPSEQYRKVFGAITTTLQFVLPFFIISICYVWISVKLNQRARAK *F PGSKSSREEADRKRTNRMLIAMVAVFGLSWLPINVVNIFDDFDDKSNEWRFYILFFFVAHSIAMSSTCYNPFLY *F >CG8422 *F VELPTIIYYIGYTLSLVSLSLALIVFAYFKELRCLRNTIHANLFFTYIMSALFWILLLSVQISIVGSCIALITLFHFFT *F LTNFFWMLVEGLYLYMLVVKTFSGDNLRFNIYASIGWGGPALFVVTWAVAKSLTVTYSTPEKYEINCPWMQETHVDWIY *F QGPVCAVLIINLTFLLRIMWVLITKLRKAAKALLVLIPLFGITYLVVLAGPSESGLMGHMFAVLRAVLLSTQGFSVSLF *F YCFLNSEV *F >CG8784 *F GNLITCIVISRNNFMHTATNFYLFNLAVSDLILLVSGIPQELYNLWYPDMYPFTDAMCIMGSVLSEMAANATVLTITAF *F TVERYIAICHPFRQHTMSKLSRAIKFIFAIWLAAFLLALPQAMQFSVVYQNEGYSCTMENDFYAHVFAVSGFIFFGGPM *F TAICVLYVLIGVKLKRSRLLQSLPRRTFDANRGLNAQGRVIRMLVAVAVAFFLCWAPFHAQRLMAVYGLNLINIGISRD *F AFNDYFRILDYTSGVLYFLSTCINPLLY *F >CG8795 *F GNLITCIVISRNNFMHTATNFYLFNLAISDMILLCSGMPQDLYNLWHPDNYPFSDSICILESVLSETAANATVLTITAF *F TVERYIAICHPFRQHTMSKLSRAVKFIFAIWIAALLLALPQAIQFSVVMQGMGTSCTMKNDFFAHVFAVSGFLFFGGPM *F TAICVLYVLIGVKLKRSRLLQALPRRCYDVNRGISAQTRVIRMLVAVAVAFFICWAPFHAQRLMAVYGSTSGIESQWFN *F DVFSILDYTSGVLYFLSTCINPLLY *F >CG8985 *F ANTLNIIVLTRREMRSPTNAILTGLAVADLAVMLEYIPYTIHDYILTDSLPREEKLSYSWACFIKFHSIFAQVLHTISI *F WLTVTLAVWRYIAVGYPQKNRVWCGMRTTIITITTAYVVCVLVVSPSLYLITAITEYVDQLDMNGKVANSIPMTQYVID *F YRNELLSARTAALNATPTSAPLNETVWLNASTLLTSTTTAAPPTPSPVVRNVTVYRLYHSDLDLHNASLQNATFLIYSV *F VIKLIPCIALTILSVRLILALLEAKRRRKKLTSKPATPGASNGTKSPANGKAADRPRKNSKTLEKEKQTDRTTRMLLAV *F LLLFLITEFPQGIMGLLNAVLGDVFYLQCYLRLSDLMDILALINSSINFILY *F >CG9918 *F GNISTCIVIKKNRSMHTATNYYLFSLAISDFLLLLSGVPQEVSYIWSKYPYVFGEYICIGRGLLAETSANATVLTITAF *F TVERYIAICHPFLGQAMSKLSRAIRIIVLVWIMAIVTAIPQAAQFGIEHYSGVEQCGIVRVIVKHSFQLSTFIFFLAPM *F SIILVLYLLIGVHLYRSTLVEGPASVARRQQLKSVPSDTILYRYGGSGTAMSFNGGGSGAGTAGLMGGSGAQLSSVRGR *F LNHYGTRRVLRMLVAVVVCFFLCWAPFHAQRLIAIYAPARGAKLRDQHEFVYTVMTYVSGVLYYLSTCINPLLY *F >AlstR *F GNGLVILVVVANQQMRSTTNLLIINLAVSDILFVIFCVPFTATDYVLPEWPFGNVWCKFVQYMIVVTCHCSVYTLVLMS *F FDRFLAVVHPVTSMSLRTERNATLAIMCAWITIVTTAIPVALSHSVRIYQYHGNAGTACVFSTEEEIWLVGFQVSFFLS *F SYVAPLTLICFLYMGMLARLWKSAPGCKPSAESRKGKRRVTRMVVVVVLAFAICWLPIHVILVLKALNLYGGSHLSVII *F QIISHVVAYTNSCINPILY *F >Fsh/CG7665 *F GNVAVLTVILSIRPESTPVPRFLMCHLAFADLCLGLYLLLVACIDAHSMGEYFNFAYDWQYGLGCKVAGFLTVFASHLS *F VFTLTVITIERWLAITQAMYLNHRIKLRPAALIMLGGWIYSMLMSSLPLFGISNYSSTSICLPMENRDVYDTIYLIAIL *F GSNGVAFSIIAVCYAQIYLSLGRETRQAHQNSPGELSVAKKMALLVFTNFACWSPIAFFGLTALAGYPLINVTKSKILL *F VFFYPLNSCADPYLY *F >GRHR/CG11325 *F GNSTVLYLLTKRRLRGPLRIDIMLMHLAIADLMVTLLLMPMEIVWAWTVQWLSTDLMCRLMSFFRVFGLYLSSYVMVCI *F SLDRYFAILKPLKRSYNRGRIMLACAWLGSVVCSIPQAFLFHLEEHPAVTGYFQCVIFNSFRSDFDEKLYQAASMCSMY *F AFPLIMFIYCYGAIYLEIYRKSQRVLKDVIAERFRRSNDDVLSRAKKRTLKMTITIVIVFIICWTPYYTISMWYWLDKH *F SAGKINPLLRKALFIFASTNSCMNPLVY *F >rk/CG8930 *F GNGTVVFVLLCSRSKMDVPRFLVCNLAAADFFMGIYLGILAIVDAATLGEFRMFAIPWQMSVLCQLSGFLAVLSSELSV *F YTLAVITLERNYAITHAIHLNKRLSLKQAGYIMSVGWVFALIMALMPLVGVSDYRKFAVCLPFETTTGPASLTYVISLM *F FINGCAFLTLMGCYLKMYWAIRGSQAWNTNDSRIAKRMALLVFTDFLCWSPIAFFSITAIFGLQLISLEQAKIFTVFVL *F PLNSCCNPFLY *F >TAKR86C *F GNGIVLWIVTGHRSMRTVTNYFLLNLSIADLLMSSLNCVFNFIFMLNSDWPFGSIYCTINNFVANVTVSTSVFTLVAIS *F FDRYIAIVDPLKRRTSRRKVRIILVLIWALSCVLSAPCLLYSSIMTKQYYNGKSRTVCFMMWPDGRYPTSMADYAYNLI *F ILVLTTGIPMIVMLICYSLMGRVLWGSRSIGENTDRQMESMKSKRKVVRMFIAIVSIFAICWLPYHLFFIYAYHNNQVA *F STKYVQHMYLGFYWLAMSNAMVNPLIY *F >TAKR99D *F GNLIVVWIVMTTKRMRTVTNYFIVNLSIADAMVSSLNVTFNYYYMLDSDWPFGEFYCKLSQFIAMLSICASVFTLMAIS *F IDRYVAIIRPLQPRMSKRCNLAIAAVIWLASTLISCPMMIIYRTEEVPVRGLSNRTVCYPEWPDGPTNHSTMESLYNIL *F IIILTYFLPIVSMTVTYSRVGIELWGSKTIGECTPRQVENVRSKRRVVKMMIVVVLIFAICWLPFHSYFIITSCYPAIT *F EAPFIQELYLAIYWLAMSNSMYNPIIY *F >CG4187 with ectodomain (partial) *F RANCDGSVDCDDASDEVNCVNEVDAKYWDHLYRKQPFGRHDNLRIGECLWPNENFSCPCRGDEILCRFQQLTDIPERLP *F QHDLATLDLTGNNFETIHETFFSELPDVDSLVLKFCSIREIASHAFDRLADNPLRTLYMDDNKLPHLPEHFFPEGNQLS *F ILDLRGNRIGNFEAEVFARLPNLEVLYLNENHLKRLDPDRFPRTLLNLHTLSLAYNQIEDIAANTFPFPRLRYLHLNEN *F RIEGFDLEAFACLKNLSSLLLTGNRFQTLDSRVLKNLTSLDYIAAMNVRVCDPHGDGISSKLHLLDNQILRGSVWVMAS *F IAVVGNLLVLLGRYFYKSRSNVEHSLYLRHLAASDFLMGIYLTLIACADISFRGEYIKYEETWRHSGVCAFAGFLSTFS *F CQSSTLLLTLVTWDRLMSVTRPLKPRDTEKVRIVLRLLLLWGISFGLAAAPLLPNPYFGSHFYGNNGVCLSLHIHDPYA *F KGWEYSALLFILVNTLSLIFILFSYIRMLQAIRDSGGGMRSTHSGRENVVATRFAIIVTTDCACWLPIIVVKLAALSGC *F EISPDLYAWLAVLVLPVNSALNPVLY *F >CG5042 with ectodomain *F MVYGRSIAVGFCLMTVVLLLAAVIFYLSLGPCPAASFACDNGTLCVPRRQMCDSRNDCADSSDENPVECGLLYGSKEIA *F DKIVRNAIEKKQQRLISAVSNASGADSTTSMVPRNQSLTLNMTCDIVTYPKACQCGQGTILYCGRYAKLRRFPRLSSEV *F TNLIIIRNNLTLRDNIFANFTRLQKLTLKYNNISRVPLGSFSGLFHLERLELSHNNVSHLPHGVFLGLHSLQWLFLVNN *F HLHHLPVEQLRFFRRLEWLVLSRNRLTLRNVQLPKIPTLYEVQLYQHQMQGHYAWPTRRMSNVKSANNSKTCVSFYSRL *F VGNPIKELSGETFLHNTRLEALSLALMPIHISSSLMEPLNISFLNLTGIRYDHIDFEAINSMRNLTYIIYDRFFYCSMT *F PRVRMCKPSTDGVSSFQDLLSKPVLRYSAWVMATLTIAGNVLVLWGRFIYRDENVAVTMVIRNLALADMLMGFYLVTIG *F VQDYRYRNEYYKVVLDWITSWQCTLIGTLAVSSSEVSMLILAFMSLERFLLIADPFRGHRSIGNRVMWLALICIWITGV *F GLAVAPVLLWRTSTLPYYGSYSGTCFPLHIHEAFPMGWLYSAFVFLGVNLLLLVMIAMLYTALLISIWRTRSATPLTLL *F DCEFAVRFFFIVLTDFLCWVPIIVMKIWVFFNYNISDDIYAWLVVFVLPLNSAVNPLLY # *U FBrf0137458 *a Misra *b S. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG1142 on Mon Jul 30 15:22:39 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 15:22:39 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG1142 on Mon Jul 30 15:22:39 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG1142 *F Release: 1 *F Gene annotation error *F Gene CG1142 has incorrect exon/intron structure or translation start site. *F Comments: CG1142 shows good match to part of the X element from 872-1536 of *F its 1536 nucleotides. *F Query= CG1142|FBgn0037504|CT1929|FBan0001142 GO: located on: 3R 84D4-84D4; *F |cDNA sequence *F (1536 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 2934 7.2e-131 *F 1 gb|X17551|Doc DMW1DOC 4725bp Derived from X17551 (g8821) ... 447 7.0e-14 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 2934 (446.3 bits), Expect = 7.2e-131, P = 7.2e-131 *F Identities = 626/669 (93%), Positives = 626/669 (93%), Strand = Plus / Plus *F Query: 872 GGAAGCCCCCGTACCACAGCCGAACGCAGCAAGGTACCTTGGAGTGCTTCTGGATCGCAG 931 *F ||||| |||||||||||||||||||||||||| ||||||||||||||||||||||||||| *F Sbjct: 4031 GGAAGTCCCCGTACCACAGCCGAACGCAGCAAAGTACCTTGGAGTGCTTCTGGATCGCAG 4090 *F Query: 932 ACTCACATTTCCCAAGCATGTGACTGACATCAGAACACGCCTACGTGCTAAGTTGGCGAA 991 *F |||||||||| ||||||||||||| ||||||||||| ||||||||||||||| ||||||| *F Sbjct: 4091 ACTCACATTTTCCAAGCATGTGACCGACATCAGAACGCGCCTACGTGCTAAGGTGGCGAA 4150 *F Query: 992 GCACTACTGGCTACTTTGTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGACAATTTA 1051 *F ||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4151 GCACTACTGGCTACTTTCTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGACAATTTA 4210 *F Query: 1052 CAAACAGATATTAGCACCAAACTGGAAGTATGGATGCCAAATCTCGGGCTTGGCATGCGA 1111 *F ||||||||| |||||||||||||||||||||| |||||||||| |||||| || ||||| *F Sbjct: 4211 CAAACAGATCCTAGCACCAAACTGGAAGTATGGGTGCCAAATCTGGGGCTTAGCCTGCGA 4270 *F Query: 1112 CAGCCAAATAAAAAGGATCCAGGCTATTCAAAATAAGGTAGCAAGACTCATCACCGGCTG 1171 *F |||||| || |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4271 CAGCCACATCAAAAGGATCCAGGCTATTCAAAATAAGGTAGCAAGACTCATCACCGGCTG 4330 *F Query: 1172 CGAGTGGTTTGTGCGAAACACCACCCTGCACAGAGACCTGAAACTCGCAACGGTATTTGA 1231 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4331 CGAGTGGTTTGTTCGAAACACCACCCTGCACAGAGACCTGAAACTCGCAACGGTATTTGA 4390 *F Query: 1232 CGAGATAAACCAGCACTCGAGCAGGTACCATGACAGGCTGGAGCGCCACAGAAATCGGCT 1291 *F ||| |||||| ||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 4391 CGAAATAAACAAGCACTCGAGCAGATACCATGACAGGCTGGAGCGCCACAGAAATCGGCT 4450 *F Query: 1292 GGCCAGCGCTCTTAACAGATCTCGCCCACCAAGGAGGCTCAACAGAAGGCAACCGAGGGA 1351 *F |||||||||| | ||||||||||||||||||||||||||||| ||||||||||||||||| *F Sbjct: 4451 GGCCAGCGCTTTAAACAGATCTCGCCCACCAAGGAGGCTCAATAGAAGGCAACCGAGGGA 4510 *F Query: 1352 TCTCATTACCCGATCTCCTTTGACAAGGGTCAGCAGAGGCTGACGCTTATCAAAAAACCT 1411 *F ||||||||||||||||||||||||||||||| ||||| ||||||||||||| ||| ||| *F Sbjct: 4511 TCTCATTACCCGATCTCCTTTGACAAGGGTCCGCAGAAGCTGACGCTTATCTTAAATCCT 4570 *F Query: 1412 ATTTGTTATATGTGTTTGTTATGTACT-GTA-TTA--TTATTGTAAACTAGAAAAAGCTA 1467 *F |||||||||||||| |||||||||| | ||| ||| ||| |||||| | |||||||||| *F Sbjct: 4571 ATTTGTTATATGTGATTGTTATGTAATTGTAGTTAAATTACTGTAAATTTGAAAAAGCTA 4630 *F Query: 1468 ACTGTAGTTAGCCGGCGCGCCCAAATGGGCAGAAATAATAGAAAAGAAGGACCCAAAGGG 1527 *F ||| ||||||||||||| |||||||||||| ||| ||||||| ||||||||| ||||||| *F Sbjct: 4631 ACTATAGTTAGCCGGCGAGCCCAAATGGGCTGAATTAATAGATAAGAAGGACACAAAGGG 4690 *F Query: 1528 GCTCCAAGA 1536 *F ||| ||||| *F Sbjct: 4691 GCTTCAAGA 4699 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137459 *a Misra *b S. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG18352 on Mon Jul 30 15:37:41 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 15:37:41 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG18352 on Mon Jul 30 15:37:41 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG18352 *F Release: 1 *F Gene annotation error *F Gene CG18352 has a mistake in the supporting evidence or functional assignment. *F Comments: CG18352 can be deleted from GadFly--it appears to be part of an X *F transposable element. *F Query= CG18352|FBgn0036130|CT41704|FBan0018352 GO: located on: 3L *F 68A4-68A4; |cDNA sequence *F (1590 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 7881 0.0 *F 1 gb|X17551|Doc DMW1DOC 4725bp Derived from X17551 (g8821) ... 523 2.2e-17 *F 1 gb|AC005734|Waldo-A WALDOA 5150bp 170 0.35 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 7881 (1188.5 bits), Expect = 0.0, P = 0.0 *F Identities = 1583/1590 (99%), Positives = 1583/1590 (99%), Strand = Plus / Plus *F Query: 1 ATGGATATGCCAATCACGCCGTTTGAACCCTGCGAGGTAGCCGAAGTCATTGTGCGCCAG 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3111 ATGGATATGCCAATCACGCCGTTTGAACCCTGCGAGGTAGCCGAAGTCATTGTGCGCCAG 3170 *F Query: 61 AGTAACAACAAAGCACCTGGACATGACGTTATCTGCAACGCCACATTGAAGGCCCTGCCC 120 *F ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 3171 AGTAACAACAAAGCACCTGGACATGACGTCATCTGCAACGCCACATTGAAGGCCCTGCCC 3230 *F Query: 121 AGACAAGCGATCCTCTACATAACGTTGGTTTTCAACGCTATTGTGAGGTTGCAATACTTC 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3231 AGACAAGCGATCCTCTACATAACGTTGGTTTTCAACGCTATTGTGAGGTTGCAATACTTC 3290 *F Query: 181 CCTTATCAGTGGAAGCTCGGGATAATCTCCATGATCCACAAACCTGGCAAGCCGGAAAGG 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3291 CCTTATCAGTGGAAGCTCGGGATAATCTCCATGATCCACAAACCTGGCAAGCCGGAAAGG 3350 *F Query: 241 GAGCCCGCCTCCTACCGGCCGATCAGTCTCCTCCCTTCAATTTCGAAGGTGTTTGAGAGA 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3351 GAGCCCGCCTCCTACCGGCCGATCAGTCTCCTCCCTTCAATTTCGAAGGTGTTTGAGAGA 3410 *F Query: 301 CTGATTGCTGTCCGGATTGTAAGGATTATGGAAGCCCAGGGGATTACCCCTGAGCACCAG 360 *F ||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 3411 CTGATTGCTGTCCGGATTGTAAGCATTATGGAAGCCCAGGGGATTACCCCTGAGCACCAG 3470 *F Query: 361 TTCGGTTTCCGTGCTGGCCACTGTACTGTCGAGCAGCTCCATCGAGTCGTCGAGCAAATT 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3471 TTCGGTTTCCGTGCTGGCCACTGTACTGTCGAGCAGCTCCATCGAGTCGTCGAGCAAATT 3530 *F Query: 421 CTGACTGCCTACGACAGTAAGGAATATTGTAACAGCCTCTTCTTGGACATTCGAGAAGCG 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3531 CTGACTGCCTACGACAGTAAGGAATATTGTAACAGCCTCTTCTTGGACATTCGAGAAGCG 3590 *F Query: 481 TTTGATCGAGTGTGGCACATTGGACTCCAACTGAAAATCAAGCAGACGCTGCCTGCTCCA 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 3591 TTTGATCGAGTGTGGCACATTGGACTCCAACTGAAAATCAAGCAGACGCTGCCTGCCCCA 3650 *F Query: 541 TATTTTGGGTTGCTGAAATCGTACCTGGAAGGAAGGAGGTTCGCTGTGCGCTTTCATTCA 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3651 TATTTTGGGTTGCTGAAATCGTACCTGGAAGGAAGGAGGTTCGCTGTGCGCTTTCATTCA 3710 *F Query: 601 GCAATTTCCACCGAGCACAACGTGGCAGCTGGTGTTCCACAAGGTAGTGTCCTCGGCCCC 660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3711 GCAATTTCCACCGAGCACAACGTGGCAGCTGGTGTTCCACAAGGTAGTGTCCTCGGCCCC 3770 *F Query: 661 CTGCTCTACTGCCTGTGTAGCCACGACATGCCGCAGCCAGATGTAAGCCTTTACGGGAAA 720 *F |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3771 CTGCTCTACTGCCTGTATAGCCACGACATGCCGCAGCCAGATGTAAGCCTTTACGGGAAA 3830 *F Query: 721 TCTATGTTGGCCACATTTGCCGATGACGTGTGCGTCACCTACAGGTCCCGATGCGAGCAC 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3831 TCTATGTTGGCCACATTTGCCGATGACGTGTGCGTCACCTACAGGTCCCGATGCGAGCAC 3890 *F Query: 781 GACGCAAGCCGATGGTATCCAGGACTTTGCATACCGGTTCTCGGAATGGGCAAGACGATG 840 *F |||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3891 GACGCA-GCCGATGGTATCCAGGACTTTGCATACCGGTTCTCGGAATGGGCAAGACGATG 3949 *F Query: 841 GAATATTGGCATCAATAGCAGTAAATCCAACAACGTCTGCTTCACTTTAAAGCGGAGAAC 900 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3950 GAATATTGGCATCAATAGCAGTAAATCCAACAACGTCTGCTTCACTTTAAAGCGGAGAAC 4009 *F Query: 901 GCCACCGCCCGTCTACATCGAGGAAGTCCCCGTACCACAGCCGAACGCAGCAAAGTACCT 960 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4010 GCCACCGCCCGTCTACATCGAGGAAGTCCCCGTACCACAGCCGAACGCAGCAAAGTACCT 4069 *F Query: 961 TGGAGTGCTTCTGGATCGCAGACTCACATTTTCCAAGCATGTGACCGACATCAGAACGCG 1020 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4070 TGGAGTGCTTCTGGATCGCAGACTCACATTTTCCAAGCATGTGACCGACATCAGAACGCG 4129 *F Query: 1021 CCTACGTGCTAAGGTGGCGAAGCACTACTGGCTACTTTCTTCGCGCAGTAAATTGTCGCT 1080 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4130 CCTACGTGCTAAGGTGGCGAAGCACTACTGGCTACTTTCTTCGCGCAGTAAATTGTCGCT 4189 *F Query: 1081 ATCCAACAAGCTGACAATTTACAAACAGATCCTAGCACCAAACTGGAAGTATGGGTGCCA 1140 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4190 ATCCAACAAGCTGACAATTTACAAACAGATCCTAGCACCAAACTGGAAGTATGGGTGCCA 4249 *F Query: 1141 AATCTGGGGCTTAGCCTGCGCCAGCCACATCAAAAGGATCCAGGCTATTCAAAATAAGGT 1200 *F |||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4250 AATCTGGGGCTTAGCCTGCGACAGCCACATCAAAAGGATCCAGGCTATTCAAAATAAGGT 4309 *F Query: 1201 AGCAAGACTCATCACCGGCTGCGAGTGGTTTGTTCGAAACACCACCCTGCACAGAGACCT 1260 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4310 AGCAAGACTCATCACCGGCTGCGAGTGGTTTGTTCGAAACACCACCCTGCACAGAGACCT 4369 *F Query: 1261 GAAACTCGCAACGGTATTTGACGAAATAAACAAGCACTCGAGCAGATACCATGACAGGCT 1320 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4370 GAAACTCGCAACGGTATTTGACGAAATAAACAAGCACTCGAGCAGATACCATGACAGGCT 4429 *F Query: 1321 GGAGCGCCACAGAAATCGGCTGGCCAGCGCTTTAAACAGATCTCGCCCACCAAGGAGGCT 1380 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4430 GGAGCGCCACAGAAATCGGCTGGCCAGCGCTTTAAACAGATCTCGCCCACCAAGGAGGCT 4489 *F Query: 1381 CAATAGAAGGCAACCGAGGGATCTCATTACCCGATCTCCTTTGACAAGGGTCCGCAGAAG 1440 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4490 CAATAGAAGGCAACCGAGGGATCTCATTACCCGATCTCCTTTGACAAGGGTCCGCAGAAG 4549 *F Query: 1441 CTGACGCTTATCTTAAATCCTATTTGTTATATGTGATTGTTATGTAATTGTAGTTAAATT 1500 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4550 CTGACGCTTATCTTAAATCCTATTTGTTATATGTGATTGTTATGTAATTGTAGTTAAATT 4609 *F Query: 1501 ACTGTAAATTTGAAAAAGCTAACTATAGTTAGCCGGCGAGCCCAAATGGGCTGAATTAAT 1560 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4610 ACTGTAAATTTGAAAAAGCTAACTATAGTTAGCCGGCGAGCCCAAATGGGCTGAATTAAT 4669 *F Query: 1561 AGATAAGAAGGACACAAAGGGGATTCAAGA 1590 *F |||||||||||||||||||||| ||||||| *F Sbjct: 4670 AGATAAGAAGGACACAAAGGGGCTTCAAGA 4699 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137460 *a Misra *b S. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG18256 on Mon Jul 30 15:39:34 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Jul 30 23:39:33 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 30 Jul 2001 23:39:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 15:39:34 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG18256 on Mon Jul 30 15:39:34 2001 *F Content-Length: 6170 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG18256 *F Release: 1 *F Gene annotation error *F Gene CG18256 has a mistake in the supporting evidence or functional assignment. *F Comments: CG18256 can be deleted from GadFly--it appears to be part of an X *F element. *F Query= CG18256|FBgn0030380|CT41371|FBan0018256 GO: located on: X *F 11A12-11A12; |cDNA sequence *F (1243 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 5969 6.0e-268 *F 1 gb|X17551|Doc DMW1DOC 4725bp Derived from X17551 (g8821) ... 525 8.9e-18 *F 1 gb|AC005734|Waldo-A WALDOA 5150bp 170 0.27 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 5969 (901.6 bits), Expect = 6.0e-268, P = 6.0e-268 *F Identities = 1199/1204 (99%), Positives = 1199/1204 (99%), Strand = Plus / Plus *F Query: 40 GTCGAGCAGCTCCATCGAGTCGTCGAGCAAATTCTGACTGCCTACGACAGTAAGGAATAT 99 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3498 GTCGAGCAGCTCCATCGAGTCGTCGAGCAAATTCTGACTGCCTACGACAGTAAGGAATAT 3557 *F Query: 100 TGTAACAGCCTCTTCTTGGACATTCGAGAAGCGTTTGATCGAGTGTGGCACATTGGACTC 159 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3558 TGTAACAGCCTCTTCTTGGACATTCGAGAAGCGTTTGATCGAGTGTGGCACATTGGACTC 3617 *F Query: 160 CAACTGAAAATCAAGCAGACGCTGCCTGCTCCATATTTTGGGTTGCTGAAATCGTACCTG 219 *F ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 3618 CAACTGAAAATCAAGCAGACGCTGCCTGCCCCATATTTTGGGTTGCTGAAATCGTACCTG 3677 *F Query: 220 GAAGGAAGGAGGTTCGCTGTGCGCTTTCATTCAGCAATTTCCACCGAGCTCAACGTGGCA 279 *F ||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 3678 GAAGGAAGGAGGTTCGCTGTGCGCTTTCATTCAGCAATTTCCACCGAGCACAACGTGGCA 3737 *F Query: 280 GCTGGTGTTCCACAAGGTAGTGTCCTCGGCCCCCTGCTCTACTGCCTGTATAGCCACGAC 339 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3738 GCTGGTGTTCCACAAGGTAGTGTCCTCGGCCCCCTGCTCTACTGCCTGTATAGCCACGAC 3797 *F Query: 340 ATGCCGCAGCCAGATGTAAGCCTTTACGGGAAATCTATGTTGGCCACATTTGCCGATGAC 399 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3798 ATGCCGCAGCCAGATGTAAGCCTTTACGGGAAATCTATGTTGGCCACATTTGCCGATGAC 3857 *F Query: 400 GTGTGCGTCACCTACAGGTCCCGATGCGAGCACGACGCAAGCCGATGGTATCCAGGACTT 459 *F ||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 3858 GTGTGCGTCACCTACAGGTCCCGATGCGAGCACGACGCA-GCCGATGGTATCCAGGACTT 3916 *F Query: 460 TGCATACCGGTTCTCGGAATGGGTAAGACGATGGAATATTGGCATCAATAGCAGTAAATC 519 *F ||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 3917 TGCATACCGGTTCTCGGAATGGGCAAGACGATGGAATATTGGCATCAATAGCAGTAAATC 3976 *F Query: 520 CAACAACGTCTGCTTCACTTTAAAGCGGAGAACGCCACCGCCCGTCTACATCGAGGAAGT 579 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3977 CAACAACGTCTGCTTCACTTTAAAGCGGAGAACGCCACCGCCCGTCTACATCGAGGAAGT 4036 *F Query: 580 CCCCGTACCACAGCCGAACGCAGCAAAGTACCTTGGAGTGCTTCTGGATCGCAGACTCAC 639 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4037 CCCCGTACCACAGCCGAACGCAGCAAAGTACCTTGGAGTGCTTCTGGATCGCAGACTCAC 4096 *F Query: 640 ATTTTCCAAGCATGTGACCGACATCAGAACGCGCCTACGTGCTAAGGTGGCGAAGCACTA 699 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4097 ATTTTCCAAGCATGTGACCGACATCAGAACGCGCCTACGTGCTAAGGTGGCGAAGCACTA 4156 *F Query: 700 CTGGCTACTTTCTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGACAATTTACAAACA 759 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4157 CTGGCTACTTTCTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGACAATTTACAAACA 4216 *F Query: 760 GATCCTAGCACCAAACTGGAAGTATGGGTGCCAAATCTGGGGCTTAGCCTGCGACAGCCA 819 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4217 GATCCTAGCACCAAACTGGAAGTATGGGTGCCAAATCTGGGGCTTAGCCTGCGACAGCCA 4276 *F Query: 820 CATCAAAAGGATCCAGGCTATTCAAAATAAGGTAGCAAGACTCATCACCGGCTGCGAGTG 879 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4277 CATCAAAAGGATCCAGGCTATTCAAAATAAGGTAGCAAGACTCATCACCGGCTGCGAGTG 4336 *F Query: 880 GTTTGTTCGAAACACCACCCTGCACAGAGACCTGAAACTCGCAACGGTATTTGACGAAAT 939 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4337 GTTTGTTCGAAACACCACCCTGCACAGAGACCTGAAACTCGCAACGGTATTTGACGAAAT 4396 *F Query: 940 AAACAAGCACTCGAGCAGATACCATGACAGGCTGGAGCGCCACAGAAATCGGCTGGCCAG 999 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4397 AAACAAGCACTCGAGCAGATACCATGACAGGCTGGAGCGCCACAGAAATCGGCTGGCCAG 4456 *F Query: 1000 CGCTTTAAACAGATCTCGCCCACCAAGGAGGCTCAATAGAAGGCAACCGAGGGATCTCAT 1059 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4457 CGCTTTAAACAGATCTCGCCCACCAAGGAGGCTCAATAGAAGGCAACCGAGGGATCTCAT 4516 *F Query: 1060 TACCCGATCTCCTTTGACAAGGGTCCGCAGAAGCTGACGCTTATCTTAAATCCTATTTGT 1119 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4517 TACCCGATCTCCTTTGACAAGGGTCCGCAGAAGCTGACGCTTATCTTAAATCCTATTTGT 4576 *F Query: 1120 TATATGTGATTGTTATGTAATTGTAGTTAAATTACTGTAAATTTGAAAAAGCTAACTATA 1179 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4577 TATATGTGATTGTTATGTAATTGTAGTTAAATTACTGTAAATTTGAAAAAGCTAACTATA 4636 *F Query: 1180 GTTAGCCGGCGAGCCCAAATGGGCTGAATTAATAGATAAGAAGGACACAAAGGGGATTCA 1239 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||| *F Sbjct: 4637 GTTAGCCGGCGAGCCCAAATGGGCTGAATTAATAGATAAGAAGGACACAAAGGGGCTTCA 4696 *F Query: 1240 AGAC 1243 *F |||| *F Sbjct: 4697 AGAC 4700 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137461 *a Misra *b S. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG18303 on Mon Jul 30 15:41:30 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Jul 30 23:41:29 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 30 Jul 2001 23:41:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 15:41:30 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG18303 on Mon Jul 30 15:41:30 2001 *F Content-Length: 3797 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG18303 *F Release: 1 *F Gene annotation error *F Gene CG18303 has a mistake in the supporting evidence or functional assignment. *F Comments: CG18303 can be deleted from GadFly--it appears to be part of an X *F element. *F Query= CG18303|FBgn0031212|CT41545|FBan0018303 GO: located on: 2L *F 21A5-21A5; |cDNA sequence *F (648 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 2500 1.8e-110 *F 1 gb|X17551|Doc DMW1DOC 4725bp Derived from X17551 (g8821) ... 324 3.0e-09 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 2500 (381.2 bits), Expect = 1.8e-110, P = 1.8e-110 *F Identities = 558/617 (90%), Positives = 558/617 (90%), Strand = Plus / Plus *F Query: 36 CATACTCAAATTTCGGATTTGTTTGATTACTACTGTTCAAAATGCAC-TACGTGCTAAGG 94 *F || ||||| |||| | | ||| | || ||| || || | |||||||||||| *F Sbjct: 4088 CAGACTCACATTTTCCAAGCATGTGAC--CGACA--TCAGAACGCGCCTACGTGCTAAGG 4143 *F Query: 95 TGGCGAGGCACTACTGGCTACTTTGTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGA 154 *F |||||| ||||||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 4144 TGGCGAAGCACTACTGGCTACTTTCTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGA 4203 *F Query: 155 CAATTTACAAACAGATATTAGCACCAAACTGGAAGTATGGGTGCCAAATCTGGGTCTTGG 214 *F |||||||||||||||| |||||||||||||||||||||||||||||||||||| ||| | *F Sbjct: 4204 CAATTTACAAACAGATCCTAGCACCAAACTGGAAGTATGGGTGCCAAATCTGGGGCTTAG 4263 *F Query: 215 CATGCGACAGCCAAATAAAAAGGATCCAGGCTATTCAAAATAAGGTAGCAAGACTCATCA 274 *F | ||||||||||| || ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4264 CCTGCGACAGCCACATCAAAAGGATCCAGGCTATTCAAAATAAGGTAGCAAGACTCATCA 4323 *F Query: 275 CCGGCTGCGAGTGGTTTGTGCGAAACACCACCCTGCACAGAGACCTGAAACTCGCAACGG 334 *F ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4324 CCGGCTGCGAGTGGTTTGTTCGAAACACCACCCTGCACAGAGACCTGAAACTCGCAACGG 4383 *F Query: 335 TATTTGACGAGATAAACCAGCACTCGAGCAGGTACCATGACAGGCTGGAGCGCCACAGAA 394 *F |||||||||| |||||| ||||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 4384 TATTTGACGAAATAAACAAGCACTCGAGCAGATACCATGACAGGCTGGAGCGCCACAGAA 4443 *F Query: 395 ATCGGCTGGCCAGCGCTCTTAACAGATCTCGCCCACCAAGGAGGCTCAACAGAAGGCAAC 454 *F ||||||||||||||||| | ||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 4444 ATCGGCTGGCCAGCGCTTTAAACAGATCTCGCCCACCAAGGAGGCTCAATAGAAGGCAAC 4503 *F Query: 455 CGAGGGATCTCATTTCCCGATCTCCTTTGACAAGGGTCAGCAGAAGCTGACGCTTATCAA 514 *F |||||||||||||| ||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 4504 CGAGGGATCTCATTACCCGATCTCCTTTGACAAGGGTCCGCAGAAGCTGACGCTTATCTT 4563 *F Query: 515 AAAACCTATTTATTATATGTGTTTGTTATGTACT-GTA-TTATAACTG-TATAAACTAGA 571 *F ||| ||||||| ||||||||| |||||||||| | ||| ||| || | | |||| | || *F Sbjct: 4564 AAATCCTATTTGTTATATGTGATTGTTATGTAATTGTAGTTA-AATTACTGTAAATTTGA 4622 *F Query: 572 AAAAGCTAACTATAGTTAGCCGGCGCGCCCAAATGGGCAGAAATAATAGAAAAGAAGGAC 631 *F ||||||||||||||||||||||||| |||||||||||| ||| ||||||| ||||||||| *F Sbjct: 4623 AAAAGCTAACTATAGTTAGCCGGCGAGCCCAAATGGGCTGAATTAATAGATAAGAAGGAC 4682 *F Query: 632 ACAAAGGGGCTCCAAGA 648 *F ||||||||||| ||||| *F Sbjct: 4683 ACAAAGGGGCTTCAAGA 4699 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137462 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG11706 on Tue Jul 31 11:54:56 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jul 31 19:54:56 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 31 Jul 2001 19:54:56 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 11:54:56 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG11706 on Tue Jul 31 11:54:56 2001 *F Content-Length: 11640 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG11706 *F Release: 1 *F Gene annotation error *F Gene CG11706 has a mistake in the supporting evidence or functional assignment. *F Comments: CG11706 can be deleted from GadFly--it appears to be part of a *F springer element. *F Query= CG11706|FBgn0029744|CT36745|FBan0011706 GO: located on: X 4D6-4D6; *F |cDNA sequence *F (1917 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F FB|FBgn0003490|springer SPRINGER 7546bp Derived from BACR... 7033 0.0 *F 3 gb|M12927|gypsy DMGYPF1A 7469bp Derived from M12927 (g157... 848 3.1e-32 *F 1 *F >FB|FBgn0003490|springer SPRINGER 7546bp Derived from BACR06P08 by Sue *F Celniker, 29 March 2001. *F Length = 7546 *F Plus Strand HSPs: *F Score = 7033 (1061.3 bits), Expect = 0.0, Sum P(3) = 0.0 *F Identities = 1413/1418 (99%), Positives = 1413/1418 (99%), Strand = Plus / Plus *F Query: 214 CATGTCGCTCCCAACCCTTCTTCTTTGTTTCCTGGCCACGACATCGGCCCACATTACTGA 273 *F || ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5686 CAGGTCGCTCCCAACCCTTCTTCTTTGTTTCCTGGCCACGACATCGGCCCACATTACTGA 5745 *F Query: 274 CTATTCCCGAGCGAATTACATTTCCCGTCATTGACGGTAAAGTCTTAGTCTGGGAGGAAT 333 *F |||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||| *F Sbjct: 5746 CTATTCCCGAGCGAATTACATT-CCCGTCATTGACGGTAAAGTCTTAGTCTGGGAGGAAT 5804 *F Query: 334 TCGCCTATGTCAGACACTCGGCTAACCTCTCCGAGTATAGGCGGGTAATTGACGAAACCG 393 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5805 TCGCCTATGTCAGACACTCGGCTAACCTCTCCGAGTATAGGCGGGTAATTGACGAAACCG 5864 *F Query: 394 ACAGCATGCTCGATATGTTCCCCCAGTCCCATATGAAGAAACTCCTGAGCGTTGATATCG 453 *F |||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 5865 ACAGCATGCTCGATATGTTCCCCCAGTCCCATATGAAGAAGCTCCTGAGCGTTGATATCG 5924 *F Query: 454 CTCACCTCCGTGACATGCTTGATTCTTTGAGCATCCATCACAGAGTGGCAAGGAGCCTAG 513 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5925 CTCACCTCCGTGACATGCTTGATTCTTTGAGCATCCATCACAGAGTGGCAAGGAGCCTAG 5984 *F Query: 514 ACTTCTTGGGAACTGCGTTAAAGGTTGTCGCAGGGACACCTGACGCGGAAGACTTCGAGA 573 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5985 ACTTCTTGGGAACTGCGTTAAAGGTTGTCGCAGGGACACCTGACGCGGAAGACTTCGAGA 6044 *F Query: 574 AAGTCAAGTTCACTGAAGCGCGGCTTGTTGATGCACACAATAGCCAAATCGAAATAAACA 633 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6045 AAGTCAAGTTCACTGAAGCGCGGCTTGTTGATGCACACAATAGCCAAATCGAAATAAACA 6104 *F Query: 634 CCAAAACACAAGTTCGAATTAACGAACTCACTGATACCATAAATAAACTTTTAAAAATTT 693 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6105 CCAAAACACAAGTTCGAATTAACGAACTCACTGATACCATAAATAAACTTTTAAAAATTT 6164 *F Query: 694 CCAAAAGCGCTCAGATTGATACAGGTCACCTGTATGAAACGCTTTCTACTCGCAACAGAA 753 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6165 CCAAAAGCGCTCAGATTGATACAGGTCACCTGTATGAAACGCTTTCTACTCGCAACAGAA 6224 *F Query: 754 TCATTGTAATGGAATTGCAAAACTTAATGCTCACTATAACCCTCGCTAAAATTAACGTAG 813 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6225 TCATTGTAATGGAATTGCAAAACTTAATGCTCACTATAACCCTCGCTAAAATTAACGTAG 6284 *F Query: 814 TGAGTCCAAACTTCTTGGACCACGCAGATCTGGAGAGTATTTGGGGCGAGGAGCCCACCA 873 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6285 TGAGTCCAAACTTCTTGGACCACGCAGATCTGGAGAGTATTTGGGGCGAGGAGCCCACCA 6344 *F Query: 874 ACACCCCCATAAGGGAGATTTTGTCCGTTGCGTCTGTAAAAGTCCTACAATCCCTTAACA 933 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6345 ACACCCCCATAAGGGAGATTTTGTCCGTTGCGTCTGTAAAAGTCCTACAATCCCTTAACA 6404 *F Query: 934 TCTTACACTTTATTATTAAATTCCCCAAGATTATCATGGCGTGCAACAAAGTCACTATCC 993 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6405 TCTTACACTTTATTATTAAATTCCCCAAGATTATCATGGCGTGCAACAAAGTCACTATCC 6464 *F Query: 994 TTCCAGTGGTACACCACGATACGGTGTTAAGGTTGAAAGATAATGTGGTAGCAGAGTGCA 1053 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6465 TTCCAGTGGTACACCACGATACGGTGTTAAGGTTGAAAGATAATGTGGTAGCAGAGTGCA 6524 *F Query: 1054 ACAGAGAAATACGCACAGTAAAGAATTGCTCCATAACACCAGGGGCAACATTTTGCCAGT 1113 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6525 ACAGAGAAATACGCACAGTAAAGAATTGCTCCATAACACCAGGGGCAACATTTTGCCAGT 6584 *F Query: 1114 TATCTTCAGTGAGCTCGTGTGCGCAGGAGCTCCACGCTGGGGTCGTAGCACATTGCGACG 1173 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6585 TATCTTCAGTGAGCTCGTGTGCGCAGGAGCTCCACGCTGGGGTCGTAGCACATTGCGACG 6644 *F Query: 1174 CACAGCAGAGTGATCTACATCCGATCACCTACGTCGACGAAGGAATAATCGTCATCAATG 1233 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6645 CACAGCAGAGTGATCTACATCCGATCACCTACGTCGACGAAGGAATAATCGTCATCAATG 6704 *F Query: 1234 ACAGACCAGCACTCGTGCGTGTGGACAATGGAACGGCCATCCACATTAGAGGCACGCACC 1293 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6705 ACAGACCAGCACTCGTGCGTGTGGACAATGGAACGGCCATCCACATTAGAGGCACGCACC 6764 *F Query: 1294 CTCATAACATTCATTGAGAGTGCCATGGTCAACGAGACCGTCTTCTTTAATCATGACATG 1353 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6765 \-TCATAACATTCATTGAGAGTGCCATGGTCAACGAGACCGTCTTCTTTAATCATGACATG 6823 *F Query: 1354 GTCCAGAATAGGGCGCCGGGAGTGGCTAATTCCCCAGTCCTTAATATCTCGATGAAACAC 1413 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6824 GTCCAGAATAGGGCGCCGGGAGTGGCTAATTCCCCAGTCCTTAATATCTCGATGAAACAC 6883 *F Query: 1414 GAGGTCCTGAGCCTCCCATACCTTCACCGTTTAAGTGAAAAGAACTTGGAGCAAATCAGG 1473 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 6884 GAGGTCCTGAGCCTCCCATACCTTCACCGTTTAAGTGAAAAGAACTTGGAGCAAATCAGG 6943 *F Query: 1474 AACTTCGAGAAGGACGTCGACGGATACCGACTAAGTCAGATAGCGCTAGTTGCGGGAGCA 1533 *F ||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| *F Sbjct: 6944 AACTTCGAGAAGGACGTCGACGGATACCGACTAAGTCAGATAGCGTTAGTTGCGGGAGCA 7003 *F Query: 1534 ATTTTCTGCGCTCTTATCTGCATCGGTTTAACCTGGCAGCGAACCACTAGGGCCAAGAAA 1593 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7004 ATTTTCTGCGCTCTTATCTGCATCGGTTTAACCTGGCAGCGAACCACTAGGGCCAAGAAA 7063 *F Query: 1594 TCTACAGCCCAACTGAAGGAAGTTCTCGCCCAAATAGG 1631 *F |||||||||||||||||||||||||||||||||||||| *F Sbjct: 7064 TCTACAGCCCAACTGAAGGAAGTTCTCGCCCAAATAGG 7101 *F Score = 1440 (222.1 bits), Expect = 0.0, Sum P(3) = 0.0 *F Identities = 312/336 (92%), Positives = 312/336 (92%), Strand = Plus / Plus *F Query: 1582 AGGGCCAAGAAATCTACAGCCCAACTGAAGGAAGTTCTCGCCCAAATAGGCACCGGTCAA 1641 *F |||| || | ||| || | | || || || ||| || || |||||||||| *F Sbjct: 7131 AGGGAATAGTTAACTA-AGTTAACCGGACTGATCGTC-CGCACACC-AG-CACCGGTCAA 7186 *F Query: 1642 ATTGCTGACCAAGCATTTGGCCGGAAGCTCATGCATAGCCGGCAGAAGCTCTGCGCATTG 1701 *F | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7187 ACTGCTGACCAAGCATTTGGCCGGAAGCTCATGCATAGCCGGCAGAAGCTCTGCGCATTG 7246 *F Query: 1702 GCAGAGGCCGCTATGATGTTTTTCCCTTTGTTAGCTTATAGTCAGTTTGATTTTGTATTC 1761 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7247 GCAGAGGCCGCTATGATGTTTTTCCCTTTGTTAGCTTATAGTCAGTTTGATTTTGTATTC 7306 *F Query: 1762 AATAAAGAGCGCATCGCGCCTTCAATCAACTCCAGCTACTGCTGTTATCATTGAATTGGT 1821 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7307 AATAAAGAGCGCATCGCGCCTTCAATCAACTCCAGCTACTGCTGTTATCATTGAATTGGT 7366 *F Query: 1822 TGGCTAGCCTTAAGGGCAGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCT 1881 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7367 TGGCTAGCCTTAAGGGCAGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCT 7426 *F Query: 1882 AGGAGAAGAGGTCTGCGGCAACCGCCCTGCCTCCCA 1917 *F |||||||||||||||||||||||||||||||||||| *F Sbjct: 7427 AGGAGAAGAGGTCTGCGGCAACCGCCCTGCCTCCCA 7462 *F Score = 1433 (221.1 bits), Expect = 2.2e-61, P = 2.2e-61 *F Identities = 303/319 (94%), Positives = 303/319 (94%), Strand = Plus / Plus *F Query: 1602 CCAACTGAAG-GAAGTTCTCG-CC-CAAATAGGCACCGGTCAAATTGCTGACCAAGCATT 1658 *F | || | || | | | ||| || || | |||||||||||| ||||||||||||||| *F Sbjct: 7 CTAAGTTAACCGGACTGATCGTCCGCACACCAGCACCGGTCAAACTGCTGACCAAGCATT 66 *F Query: 1659 TGGCCGGAAGCTCATGCATAGCCGGCAGAAGCTCTGCGCATTGGCAGAGGCCGCTATGAT 1718 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 67 TGGCCGGAAGCTCATGCATAGCCGGCAGAAGCTCTGCGCATTGGCAGAGGCCGCTATGAT 126 *F Query: 1719 GTTTTTCCCTTTGTTAGCTTATAGTCAGTTTGATTTTGTATTCAATAAAGAGCGCATCGC 1778 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 127 GTTTTTCCCTTTGTTAGCTTATAGTCAGTTTGATTTTGTATTCAATAAAGAGCGCATCGC 186 *F Query: 1779 GCCTTCAATCAACTCCAGCTACTGCTGTTATCATTGAATTGGTTGGCTAGCCTTAAGGGC 1838 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 187 GCCTTCAATCAACTCCAGCTACTGCTGTTATCATTGAATTGGTTGGCTAGCCTTAAGGGC 246 *F Query: 1839 AGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCTAGGAGAAGAGGTCTGCG 1898 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247 AGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCTAGGAGAAGAGGTCTGCG 306 *F Query: 1899 GCAACCGCCCTGCCTCCCA 1917 *F ||||||||||||||||||| *F Sbjct: 307 GCAACCGCCCTGCCTCCCA 325 *F Score = 1045 (162.8 bits), Expect = 0.0, Sum P(3) = 0.0 *F Identities = 213/218 (97%), Positives = 213/218 (97%), Strand = Plus / Plus *F Query: 1 TGGCTAGCCTTAAGGGCAGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCT 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230 TGGCTAGCCTTAAGGGCAGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCT 289 *F Query: 61 AGGAGAAGAGGTCTGCGGCAACCGCCCTGCCTCCCAGTGACAGAGGAACCCCCTGTTACC 120 *F |||||||||||||||||||||||||||||||||||||||||||| |||||||| |||||| *F Sbjct: 290 AGGAGAAGAGGTCTGCGGCAACCGCCCTGCCTCCCAGTGACAGAAGAACCCCCCGTTACC 349 *F Query: 121 TGCAACCTACGCCGGAGACCGCGGCGAGGGACCTGCACAATACATTTAATTAATTGGCAC 180 *F |||||||||||||||||||||||||||||||||||||| || ||||||||||||||||| *F Sbjct: 350 TGCAACCTACGCCGGAGACCGCGGCGAGGGACCTGCACCTTATATTTAATTAATTGGCAC 409 *F Query: 181 CCAACTCCAGGAACCCACACCACTACCCTGAATCATGT 218 *F |||||||||||||||||||||||||||||||||||||| *F Sbjct: 410 CCAACTCCAGGAACCCACACCACTACCCTGAATCATGT 447 *F Score = 830 (130.6 bits), Expect = 2.1e-31, P = 2.1e-31 *F Identities = 170/175 (97%), Positives = 170/175 (97%), Strand = Plus / Plus *F Query: 1 TGGCTAGCCTTAAGGGCAGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCT 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7367 TGGCTAGCCTTAAGGGCAGTCAACAACGGAGAGACGTTCTCCCACCATATCTCCCAATCT 7426 *F Query: 61 AGGAGAAGAGGTCTGCGGCAACCGCCCTGCCTCCCAGTGACAGAGGAACCCCCTGTTACC 120 *F |||||||||||||||||||||||||||||||||||||||||||| |||||||| |||||| *F Sbjct: 7427 AGGAGAAGAGGTCTGCGGCAACCGCCCTGCCTCCCAGTGACAGAAGAACCCCCCGTTACC 7486 *F Query: 121 TGCAACCTACGCCGGAGACCGCGGCGAGGGACCTGCACAATACATTTAATTAATT 175 *F |||||||||||||||||||||||||||||||||||||| || |||||||||||| *F Sbjct: 7487 TGCAACCTACGCCGGAGACCGCGGCGAGGGACCTGCACCTTATATTTAATTAATT 7541 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137463 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG12574 on Tue Jul 31 11:56:37 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 11:56:37 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG12574 on Tue Jul 31 11:56:37 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG12574 *F Release: 1 *F Gene annotation error *F Gene CG12574 has a mistake in the supporting evidence or functional assignment. *F Comments: CG12574 can be deleted from GadFly--it appears to be part of a *F Waldo-A-like element (82% identity but *F over whole length). *F Query= CG12574|FBgn0040015|CT34343|FBan0012574 GO: located on: U; |cDNA *F sequence *F (375 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 810 9.0e-60 *F 2 gb|AJ278684|pilger DME278684 5108bp Derived from AJ278684... 641 1.5e-25 *F 1 gb|AC005847|Waldo-B WALDOB 5180bp 346 3.6e-12 *F 1 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 810 (127.6 bits), Expect = 9.0e-60, Sum P(2) = 9.0e-60 *F Identities = 196/237 (82%), Positives = 196/237 (82%), Strand = Plus / Plus *F Query: 138 ATCCTCCATGATCCTAGACGAGTCAGGTATCAAGTGTTGCAGCTCTCTCCAAGTCCAGGA 197 *F ||||| | || | | || ||| |||||| || |||||||||||||| ||| |||| *F Sbjct: 2010 ATCCTTGACGAGACAGGTAAAG-CAGCTATCAAATGCTGCAGCTCTCTCCACGTCGAGGA 2068 *F Query: 198 ACTGGCTGCATCACCGATGCGGGAAATCGCCTATGCAAAGATAAAACACGTGCATATGTA 257 *F ||||||||| | ||| ||||||| ||||| ||||| ||| ||||||||||||| |||| *F Sbjct: 2069 ACTGGCTGCTTTACCTATGCGGGGTATCGCTTATGCGAAGTTAAAACACGTGCACTTGTA 2128 *F Query: 258 CAGCCGCTATGCTCCGCCTAGCGACACTCCCAACCAGTTCGAGGAGTTCCTGGAGGCGCT 317 *F |||| |||| |||||||| |||||||| ||| | |||||||||||||| ||||||||||| *F Sbjct: 2129 CAGCTGCTACGCTCCGCCGAGCGACACCCCCGATCAGTTCGAGGAGTTTCTGGAGGCGCT 2188 *F Query: 318 TGTGGTCCATGCGAGAGGACGAAGCCCGAAGATCATTGCAGGTGATTTCAATGCCTG 374 *F |||| |||||||||||| |||||||||||| ||||||| || || || |||||||| *F Sbjct: 2189 CGTGGACCATGCGAGAGGGCGAAGCCCGAAGGTCATTGCCGGCGACTTTAATGCCTG 2245 *F Score = 668 (106.3 bits), Expect = 9.0e-60, Sum P(2) = 9.0e-60 *F Identities = 148/166 (89%), Positives = 148/166 (89%), Strand = Plus / Plus *F Query: 1 ATGCACGCCCATATTAGCGTAATACAGCTCAATGTTAATTATTGCGCAGCAGCTCAGAGC 60 *F |||||| | |||||||||||| ||||||||||||| ||| |||||||||||||||||||| *F Sbjct: 1863 ATGCACACTCATATTAGCGTAGTACAGCTCAATGTCAATCATTGCGCAGCAGCTCAGAGC 1922 *F Query: 61 TTTCTGGCTCAGACTGGGGCGGAGCGCAATGTAGACATCATGCTCCTAAGCGAACCTTAC 120 *F | ||||| ||||||| ||| ||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 1923 CTCCTGGCCCAGACTGCGGCTGAGCGCAATGTAGACATCATGCTCCTAAGCGAACCCTAC 1982 *F Query: 121 GTCACTGGAACGGGACAATCCTCCATGATCCTAGACGAGTCAGGTA 166 *F ||| |||| | |||||||| ||||||||||| |||||| |||||| *F Sbjct: 1983 GTCTCTGGTAGCGGACAATCGTCCATGATCCTTGACGAGACAGGTA 2028 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137464 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG15054 on Tue Jul 31 12:06:54 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 12:06:54 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG15054 on Tue Jul 31 12:06:54 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG15054 *F Release: 1 *F Gene annotation error *F Gene CG15054 has a mistake in the supporting evidence or functional assignment. *F Comments: CG15054 can be deleted from Gadfly--it appears to be part of a *F transpac transposon. *F Query= CG15054|FBgn0040880|CT34921|FBan0015054 GO: located on: X *F 17B1-17B1; |cDNA sequence *F (2819 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF222049|Transpac 5249bp 6628 0.0 *F 3 gb|AJ000387|ZAM DMZAM 8435bp Derived from AJ000387 (e1237... 1406 3.3e-97 *F 3 gb|X01472|17.6 DMIS176 7439bp AKA(J01060,J01061) Derived ... 2151 6.0e-94 *F 1 gb|X03431|297 DMIS297 6995bp Derived from X03431 (g8146) ... 2145 *F 1.0e-93 1 gb|AF364550|cruiser CRUISER 7387bp Derived from P1 clones... 2056 *F 1.2e-89 1 FB|FBgn0000199|blood BLOOD 7410bp *F 984 5.3e-38 1 gb|X59545|mdg1 DMRTMGD1 7480bp Derived from X59545 (g8507... *F 851 1.7e-36 2 FB|FBgn0020938|yoyo DM_YOYO 7521bp *F 935 9.4e-36 1 FB|FBgn0001207|HMS-Beagle Beagle 7062bp *F 887 1.5e-33 1 gb|AF315785|midline 4617bp Derived from AF315785.1 (Rel. *F ... 880 2.6e-33 1 gb|X95908|mdg3 DMMDG3 5519bp Derived from X95908 *F (e990667... 780 1.1e-28 1 gb|X04132|412 DMRT412G 6897bp AKA(X03733) *F Derived from X0... 366 9.9e-10 1 gb|M12927|gypsy DMGYPF1A 7469! *F bp Derived from M12927 (g157... 239 0.00062 1 gb|AJ009736|Idefix DME9736 *F 7411bp Derived from AJ009736 (... 202 0.030 1 gb|X14037|micropia DMDM11 *F 5457bp Derived from X14037 (g10... 161 0.89 1 gb|X93507|Tirant *F DMTIRLTR 5184bp Derived from X93507 (e10... 160 0.91 1 *F >gb|AF222049|Transpac 5249bp *F Length = 5249 *F Plus Strand HSPs: *F Score = 6628 (1000.5 bits), Expect = 0.0, Sum P(3) = 0.0 *F Identities = 1388/1441 (96%), Positives = 1388/1441 (96%), Strand = Plus / Plus *F Query: 359 ATAAATGAGCTTATACAAAAATTCGAACATATTTTTTACAAAGAAGGCCAAGACCTAAGT 418 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2014 ATAAATGAGCTTATACAAAAATTCGAACATATTTTTTACAAAGAAGGCCAAGACCTAAGT 2073 *F Query: 419 TTCACAAGTGAAATCAAACACAGGATTATTACAAAGAATGATCTTCCCATTTATTCGAAA 478 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2074 TTCACAAGTGAAATCAAACACAGGATTATTACAAAGAATGATCTTCCCATTTATTCGAAA 2133 *F Query: 479 ACATACAAATACCCAGAAATTCACAAAGACGAAGTTAACCGGCAAATAGCAGAAATGTTA 538 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2134 ACATACAAATACCCAGAAATTCACAAAGACGAAGTTAACCGGCAAATAGCAGAAATGTTA 2193 *F Query: 539 GATCAAGGAATTATAAGACACTCTAAAAGCCCATATAATAGTCCTTTGTGGGTTGTTCAG 598 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2194 GATCAAGGAATTATAAGACACTCTAAAAGCCCATATAATAGTCCTTTGTGGGTTGTTCAG 2253 *F Query: 599 AAAAAAATGGACCAGTCCAATAAGCAAAAGTGGCGGCTCGTCGTAGACTACCGTAAACTT 658 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2254 AAAAAAATGGACCAGTCCAATAAGCAAAAGTGGCGGCTCGTCGTAGACTACCGTAAACTT 2313 *F Query: 659 AATAAGGAAACCATTGAAGATCGATTCCCGATCCCAAATATAGACGAGATTTTCGACAAA 718 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2314 AATAAGGAAACCATTGAAGATCGATTCCCGATCCCAAATATAGACGAGATTTTCGACAAA 2373 *F Query: 719 TTGGGCGACTGTAAAATTTTTTCAACTTTAGACCTTGCAAAAGGTTTCTATCAGATAGAA 778 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2374 TTGGGCGACTGTAAAATTTTTTCAACTTTAGACCTTGCAAAAGGTTTCTATCAGATAGAA 2433 *F Query: 779 ATGGACAACAAAGATGTGCACAAAACAGCCTTTTCAACAACCAGTGGCCATTACGAGTTC 838 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2434 ATGGACAACAAAGATGTGCACAAAACAGCCTTTTCAACAACCAGTGGCCATTACGAGTTC 2493 *F Query: 839 CTTCGTATGCCTTTCGGACTACGGAACGCACCTTCAACATTTCAAAGGCTAATGAATAAT 898 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2494 CTTCGTATGCCTTTCGGACTACGGAACGCACCTTCAACATTTCAAAGGCTAATGAATAAT 2553 *F Query: 899 ATCCTTAGCCCCTATACCGGACAATTTTGCATTGTTTACATGGATGACATCCTTATATTT 958 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2554 ATCCTTAGCCCCTATACCGGACAATTTTGCATTGTTTACATGGATGACATCCTTATATTT 2613 *F Query: 959 TCTAAGAATATAGGGGAACACGTCAATCATTTAAGCTGTATCTTCAGCTGTCTATCTAAG 1018 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2614 TCTAAGAATATAGGGGAACACGTCAATCATTTAAGCTGTATCTTCAGCTGTCTATCTAAG 2673 *F Query: 1019 GCAAATTTAAAACTCCAATCAGACAAATGCGAGTTTGCCAGAGAGGAGATTGAATTTCTC 1078 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2674 GCAAATTTAAAACTCCAATCAGACAAATGCGAGTTTGCCAGAGAGGAGATTGAATTTCTC 2733 *F Query: 1079 GGACATACCATTAATTCAGAAGGTCTAAAGCCAAGTCAGAAGAAAATTGACGCAATTTGT 1138 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2734 GGACATACCATTAATTCAGAAGGTCTAAAGCCAAGTCAGAAGAAAATTGACGCAATTTGT 2793 *F Query: 1139 AAGATAAATTTACCGACAAACCAGAAACAAATTAAAAGTTTTCTAGGCATCACAGGTTAT 1198 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2794 AAGATAAATTTACCGACAAACCAGAAACAAATTAAAAGTTTTCTAGGCATCACAGGTTAT 2853 *F Query: 1199 TTGAGGCGCTACATAAAAGACTACTCCAAAATTGCCCAGCCCTTAATAAAATACTTAAAG 1258 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2854 TTGAGGCGCTACATAAAAGACTACTCCAAAATTGCCCAGCCCTTAATAAAATACTTAAAG 2913 *F Query: 1259 AAAAATTCTAAAATAAACACACATGATCAAGAATACGTAGAAGCTTTTCAAAAACTAAAA 1318 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 2914 AAAAATTCTAAAATAAACACACATGATCAANAATACGTAGAAGCTTTTCAAAAACTAAAA 2973 *F Query: 1319 ATCCTAATAACAAGCGATCCGATAGTCGTTTACCCAGATTTTAACAAACAATTCACGATA 1378 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2974 ATCCTAATAACAAGCGATCCGATAGTCGTTTACCCAGATTTTAACAAACAATTCACGATA 3033 *F Query: 1379 GTTACAGACGCAAGTAACTATGCATTAGGCGCCGTGCTCATGCAAGATAATAAAGTAATT 1438 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3034 GTTACAGACGCAAGTAACTATGCATTAGGCGCCGTGCTCATGCAAGATAATAAAGTAATT 3093 *F Query: 1439 TCTTATGCTAGTCGTTCTCTTAAAAATCATGAACTTAATTACAGTACAATAGAAAAAGAG 1498 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3094 TCTTATGCTAGTCGTTCTCTTAAAAATCATGAACTTAATTACAGTACAATAGAAAAAGAG 3153 *F Query: 1499 CTTCTTGCAATCTATTGGTCAACAAAATTCTTTAAATATTATATTTATGGAAGAAAGTTT 1558 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3154 CTTCTTGCAATCTATTGGTCAACAAAATTCTTTAAATATTATATTTATGGAAGAAAGTTT 3213 *F Query: 1559 ATAATTAAAACCGACCATAGACCCCTAGTTTGGCTTAATAATCTAAAGGAACCCAACCTA 1618 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3214 ATAATTAAAACCGACCATAGACCCCTAGTTTGGCTTAATAATCTAAAGGAACCCAACCTA 3273 *F Query: 1619 AAGTTACAACGTTGGAAAGTGCAATTAAACGAGTTTGATTTTGACATAACATTTATAAAA 1678 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3274 AAGTTACAACGTTGGAAAGTGCAATTAAACGAGTTTGATTTTGACATAACATTTATAAAA 3333 *F Query: 1679 GA-AATTAATAA-CTT-AGATATATTTATGCTAG-AGAACACTAACA-CATTACCGGAAG 1733 *F | || || | ||| || | | | ||| |||| | | | ||| | | |||| *F Sbjct: 3334 GGTAAAGAAAACGCTTTAGC-AGACGGACT-TAGCAGAATTGTCAAAGCATCAACAGAAG 3391 *F Query: 1734 TTACGCCTAAGACAAAAATTATAAATGAAGATTTCTCGAAAGATCTAA-TTC-ATATATT 1791 *F | | || || |||||| || || || | |||| ||| || ||||||| *F Sbjct: 3392 \--A-G--TATGATAAAAATGCCCAAACAA-ATATA--GAAATTAATAACTTAGATATATT 3443 *F Query: 1792 T 1792 *F | *F Sbjct: 3444 T 3444 *F Score = 5631 (850.9 bits), Expect = 0.0, Sum P(3) = 0.0 *F Identities = 1171/1206 (97%), Positives = 1171/1206 (97%), Strand = Plus / Plus *F Query: 1620 AGTTA-CAACGTTGG-AAAGTG-CAATTAAACGAGTTTGATTT---TGACATAACATTTA 1673 *F | ||| || ||| |||| ||| || |||| |||| || | |||| || *F Sbjct: 3360 ACTTAGCAGAATTGTCAAAGCATCAACAGAA-GAGTATGATAAAAATGCCCAAACAAATA 3418 *F Query: 1674 TAAAAGAAATTAATAACTTAGATATATTTATGCTAGAGAACACTAACACATTACCGGAAG 1733 *F || ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3419 TA---GAAATTAATAACTTAGATATATTTATGCTAGAGAACACTAACACATTACCGGAAG 3475 *F Query: 1734 TTACGCCTAAGACAAAAATTATAAATGAAGATTTCTCGAAAGATCTAATTCATATATTTG 1793 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3476 TTACGCCTAAGACAAAAATTATAAATGAAGATTTCTCGAAAGATCTAATTCATATATTTG 3535 *F Query: 1794 CCAACGATATTGACGATTTGGAAACTATTCACAGTGCGGACTCAGACGATCTTAATTTTA 1853 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3536 CCAACGATATTGACGATTTGGAAACTATTCACAGTGCGGACTCAGACGATCTTAATTTTA 3595 *F Query: 1854 TTAGTATTACAACTAATTGTCTAAACGTTTTTAAAATCCAAATACAAATAATAGAAGCGG 1913 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3596 TTAGTATTACAACTAATTGTCTAAACGTTTTTAAAATCCAAATACAAATAATAGAAGCGG 3655 *F Query: 1914 AAAGCGATTTAAGTACCTTCAAAATTTTGCATAACAAGAAAATACGACATATCTTTAAAT 1973 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3656 AAAGCGATTTAAGTACCTTCAAAATTTTGCATAACAAGAAAATACGACATATCTTTAAAT 3715 *F Query: 1974 CAAGCGGAAATCAGGAAAATATGCTTAAGTTCATGCAAGAGAAACTGCCAGAAAAAGGCT 2033 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3716 CAAGCGGAAATCAGGAAAATATGCTTAAGTTCATGCAAGAGAAACTGCCAGAAAAAGGCT 3775 *F Query: 2034 TAGTTGTAATATTTTGTGAAAATTTAAGTTTATTTGTAAAATTTCAAGAAATTTATAGGC 2093 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3776 TAGTTGTAATATTTTGTGAAAATTTAAGTTTATTTGTAAAATTTCAAGAAATTTATAGGC 3835 *F Query: 2094 AGTATTTTTCAAGTAATAAAAATTTAAGAATTCTAAAGTCAGGTACATTGTTAGAAGACA 2153 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3836 AGTATTTTTCAAGTAATAAAAATTTAAGAATTCTAAAGTCAGGTACATTGTTAGAAGACA 3895 *F Query: 2154 TAAATGATAAAGAAAAACTCCTGAAGATTATCGAAAACGAACACATAAAGAATAATCCCA 2213 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| || *F Sbjct: 3896 TAAATGATAAAGAAAAACTCCTGAAGATTATCGAAAACGAACACATAAAGAATAATCACA 3955 *F Query: 2214 GAGGCATTAACGAAATTTTTATATCTATAAGAGAAAAATACTATTATCCAAAAATGCAAA 2273 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3956 GAGGCATTAACGAAATTTTTATATCTATAAGAGAAAAATACTATTATCCAAAAATGCAAA 4015 *F Query: 2274 AGTTTATTCAAAATTATATTAACAATTGTAAAATTTGTAATTTAGCCCAAATATGGATAG 2333 *F ||||||||||||||||||||||||||||||||||||||||||||||| ||||||| |||| *F Sbjct: 4016 AGTTTATTCAAAATTATATTAACAATTGTAAAATTTGTAATTTAGCC-AAATATG-ATAG 4073 *F Query: 2334 CCAACCTATAAAATATAATTTTTAATATTACAGAAACGCCAGATAAAATAAACGATATAA 2393 *F ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||| *F Sbjct: 4074 ACAACCTATAAAATATAATTTT-AATATTACAGAAACGCCAGATAAAATAAACGATATAA 4132 *F Query: 2394 TTCACATAGACATCTGGTATCCTAAAAGAAACATTATGTACGTTACATCAATTGACAAAA 2453 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4133 TTCACATAGACATCTGGTATCCTAAAAGAAACATTATGTACGTTACATCAATTGACAAAA 4192 *F Query: 2454 TGTCTAAATATGCCACTGCTCAACATATTAAAGACAGATCTTGGATTTCTTTGCTTAACG 2513 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4193 TGTCTAAATATGCCACTGCTCAACATATTAAAGACAGATCTTGGATTTCTTTGCTTAACG 4252 *F Query: 2514 CAATCAAATTAAGAATTCAATACTTAGGAAAGCCGAAAAAGATTGTAACTGATAACGAAT 2573 *F |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 4253 CAATCAAATTAAGAATTCAATACTTAGGAAAGCCTAAAAAGATTGTAACTGATAACGAAT 4312 *F Query: 2574 TCGATATTGTCGTAATTAAGCAATTTCTCCTCGAAAATAACATAGATATACACCTTTACA 2633 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| *F Sbjct: 4313 TCGATATTGTCGTAATTAAGCAATTTCTCCTCGAAAATAACATAGATATACAC-TTTACA 4371 *F Query: 2634 ACTCCTTATAAGAAAACTGGAAACTCTGATGTGGAAAGATTGCACTTAACCCTAAATGAA 2693 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4372 ACTCCTTATAAGAAAACTGGAAACTCTGATGTGGAAAGATTGCACTTAACCCTAAATGAA 4431 *F Query: 2694 CATATGCGATTGTATAATGCTGATCCAAACAATTTTGACACTATTCAAGAAAATGTTTAT 2753 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4432 CATATGCGATTGTATAATGCTGATCCAAACAATTTTGACACTATTCAAGAAAATGTTTAT 4491 *F Query: 2754 AAAGCAATTGTTTGCTATAATAACACAATTCATTCAACCACAAATATTAGACCGATAGAT 2813 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4492 AAAGCAATTGTTTGCTATAATAACACAATTCATTCAACCACAAATATTAGACCGATAGAT 4551 *F Query: 2814 TTATTT 2819 *F |||||| *F Sbjct: 4552 TTATTT 4557 *F Score = 1786 (274.0 bits), Expect = 0.0, Sum P(3) = 0.0 *F Identities = 360/362 (99%), Positives = 360/362 (99%), Strand = Plus / Plus *F Query: 1 GGTACACAACCGGATACTGATCCATGACGACAAAACATACACCACATTAGAAGTGAACCG 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1045 GGTACACAACCGGATACTGATCCATGACGACAAAACATACACCACATTAGAAGTGAACCG 1104 *F Query: 61 AATTGCACTCCGCGTTTTTAGAGACAACTTGCCTGAACCTACAAAAACGTTAATATTCGC 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1105 AATTGCACTCCGCGTTTTTAGAGACAACTTGCCTGAACCTACAAAAACGTTAATATTCGC 1164 *F Query: 121 GAGAAACCCAAATTCCGTAGAGGATGCCTATAAAATCATTGAAGATGCTCGGCATCAAAG 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1165 GAGAAACCCAAATTCCGTAGAGGATGCCTATAAAATCATTGAAGATGCTCGGCATCAAAG 1224 *F Query: 181 CTACACATTATACGGACCAATTCGAAGAAATAATAGGTACAATAAACCCAACTTCAGAAC 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1225 CTACACATTATACGGACCAATTCGAAGAAATAATAGGTACAATAAACCCAACTTCAGAAC 1284 *F Query: 241 CAATTTCAGTAATGATAATAGAAATGTAAACGAAC-AGTCGTTACAGAAAGAAGCCTTGA 299 *F ||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||| *F Sbjct: 1285 CAATTTCAGTAATGATAATAGAAATGTAAACGAACCAGTCGTTACAGAAAGAAGCCTTGA 1344 *F Query: 300 TGAAGCCAATAGGTTTCAACAAAATAATGTTGAACAACAAGAAAATTCAAGAACTGAAGA 359 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1345 TGAAGCCAATAGGTTTCAACAAAATAATGTTGAACAACAAGAAAATTCAAGAACTGAAGG 1404 *F Query: 360 TA 361 *F || *F Sbjct: 1405 TA 1406 *F Score = 167 (31.1 bits), Expect = 1.4e-81, Sum P(2) = 1.4e-81 *F Identities = 341/637 (53%), Positives = 341/637 (53%), Strand = Plus / Plus *F Query: 1915 AAGCGATTTAAGTACCTTCAAAAT--T-TTGCATAACAAGAAAATACGACATATCTT--T 1969 *F |||| | | | || | |||||| | ||| | ||||||||||| | | | | | *F Sbjct: 1347 AAGCCAAT-AGGTTTCAACAAAATAATGTTGAACAACAAGAAAATTCAAGAACTGAAGGT 1405 *F Query: 1970 AAATCAAGCGGAAATCAG-GAAAATATGCTTAAGTTCATGCAAGAGAAACTGCCAGAAAA 2028 *F | | || | |||| | ||| | | || || ||| ||| | || | *F Sbjct: 1406 ACGCCTAGGGTAAATAATAGAAGGCAGGGATATCAATAT-CAATCGAA--TAATAGCTCA 1462 *F Query: 2029 AGGCTTAGTTGTAATATTTTGTGAAAATTTAAGTTTATTTGTA-AAATTTCA-AGAAATT 2086 *F | || || | | | | || | || || | ||| ||| || || || *F Sbjct: 1463 ACTTCTAATTCTGCTCGAGTCTCAACAAATA-GTCGAAATGTGCAAAGCTCTGAGCAAAG 1521 *F Query: 2087 TATAGGCAGTATTTTTCAAGT-AATAAAAATTTAAGAATTCTAAAGTCAGGTACATTGTT 2145 *F | ||| ||| ||| | || ||| | ||| | ||| | |||| ||| ||| | *F Sbjct: 1522 CA--GGC-GTAGTTT-CGAGCCAATGGATATTAATCAAT-C--AAGT---GTAAATTTTC 1571 *F Query: 2146 AGA-AGAC-ATAAATGATAAAGAAAAACTCCTGAAGATTATCG-AAAACGAACACATAAA 2202 *F ||| ||| || || || || | | | |||| | | | |||| || ||| | *F Sbjct: 1572 AGATAGAGGATCAA-GACGAATACCATATATAGAAGTTCAAGGTAAAAAGAGGCCATTAT 1630 *F Query: 2203 GAATAATCCCAGAGGCATTAACGAAATTTTTATATCTATAAGAGAAAAATACTATTATCC 2262 *F | | || || | | | |||| | | |||| || || | | | | || *F Sbjct: 1631 TATTTATTATTGACACTGGAGCAGAATTCAG-TGTA-ATAAATGATAATTTGTGTCACCC 1688 *F Query: 2263 AAAAATGCAAA-AGTTTATTCAAA-ATTA-TATTAACAATTGTAAA-ATTTGTAATTTAG 2318 *F || | ||| || ||| | | | | || | |||| | ||| | ||| || *F Sbjct: 1689 GAAGTGGAAAACAGAC-ATTGACATAGAAGTAAAAGCAATGGGAAACAAAATTAAAATAA 1747 *F Query: 2319 CCCAAATATGGA--TAGCCAACCTAT-AA-AATAT-AATTTTTA----ATATTACAGAAA 2369 *F || |||| | | ||| | | || ||| | || | | ||||| ||| *F Sbjct: 1748 GAAAATTATGCAGGTTTCCATGTTTTCAAGAATTTGAAGCTGAAGGTTATATTTGTGAAT 1807 *F Query: 2370 CGCCAGA-TAAAATAAACGATA-TAATTCACATAGAC-ATCTGGTATCCTAAAAGAAACA 2426 *F | ||| || ||| | | || ||| || | | | ||| | ||| | | | *F Sbjct: 1808 TTCTAGAGTATAATTTTCACAACTATTTCG-ATGGGCTAATTGGC-TG-TAACATA--CT 1862 *F Query: 2427 TTATGTACGTTACATCAATTGACAAAATGTCTAAATATGCCACTGCTCAACATATTAAAG 2486 *F | ||| || || ||| | | | | | || || | | ||||| | ||| | *F Sbjct: 1863 TAATG-AC-TTGCATGCAGTCATCGATTA-CTCAA-ACAAAAAGATTCAACTCAATAATG 1918 *F Query: 2487 ACAGATCTTGGATTTCTTTGCTTAACGCAATCAAATT 2523 *F || || ||| | || | || | | || | | || *F Sbjct: 1919 TTT-AT-TTAGATCTTTTAG-TTCAGGAAAACCACTT 1952 # *U FBrf0137465 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG18665 on Tue Jul 31 12:22:29 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 12:22:29 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG18665 on Tue Jul 31 12:22:29 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG18665 *F Release: 1 *F Gene annotation error *F Gene CG18665 has a mistake in the supporting evidence or functional assignment. *F Comments: CG18665 can be deleted from GadFly--it appears to be part of an X *F element. *F Query= CG18665|FBgn0040969|CT42595|FBan0018665 GO: located on: 2L *F 33B3-33B3; |cDNA sequence *F (2775 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 10641 0.0 *F 2 gb|X17551|Doc DMW1DOC 4725bp Derived from X17551 (g8821) ... 509 5.2e-25 *F 2 gb|AC005734|Waldo-A WALDOA 5150bp 169 0.60 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 10641 (1602.6 bits), Expect = 0.0, Sum P(2) = 0.0 *F Identities = 2135/2142 (99%), Positives = 2135/2142 (99%), Strand = Plus / Plus *F Query: 634 TCGCAACTGGAGTCCACACTGCCCCTCAACACTGCCATAAACTCTGGACAGGACGTTGAT 693 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2559 TCGCAACTGGAGTCCACACTGCCCCTCAACACTGCCATAAACTCTGGACAGGACGTTGAT 2618 *F Query: 694 GATGCTATCGAACTGCTCACCAACAATATCAAGTCAGCAGCTAGATTGGCAACTCGCAGC 753 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2619 GATGCTATCGAACTGCTCACCAACAATATCAAGTCAGCAGCTAGATTGGCAACTCGCAGC 2678 *F Query: 754 ATATCTCGGCAGCCCGCGGCAGATCGAATCCCAATACCCAGGGAGATCCTGCTGCTTATA 813 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2679 ATATCTCGGCAGCCCGCGGCAGATCGAATCCCAATACCCAGGGAGATCCTGCTGCTTATA 2738 *F Query: 814 GCTGAGAAGAGGCGCTTACGCACTAGGTGGATGAGGTCTCGGCACCCGTCGGACAAAACG 873 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2739 GCTGAGAAGAGGCGCTTACGCACTAGGTGGATGAGGTCTCGGCACCCGTCGGACAAAACG 2798 *F Query: 874 GAATGGAACCGAGCTCTGAGTAGGCTCCGATGCGCGTTGGTGCTGCACAAAGCCGCATGG 933 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2799 GAATGGAACCGAGCTCTGAGTAGGCTCCGATGCGCGTTGGTGCTGCACAAAGCCGCATGG 2858 *F Query: 934 TTCGACGAAAGGCTTGCCAATACCGGAGTCGAAAGCGAAGCGACGCATTCGCTGTGGAAG 993 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2859 TTCGACGAAAGGCTTGCCAATACCGGAGTCGAAAGCGAAGCGACGCATTCGCTGTGGAAG 2918 *F Query: 994 GCCACGCGCGCAATCAAAAGGCGTTGCACGAGGAAGGCGCCTCTAGTCGATAGCAACGGG 1053 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2919 GCCACGCGCGCAATCAAAAGGCGTTGCACGAGGAAGGCGCCTCTAGTCGATAGCAACGGG 2978 *F Query: 1054 ACATGGTGTCGGACCGACTTGGGACAAGCGGAGGTATTCGCTGCGCACCTCGCCGAGCGA 1113 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2979 ACATGGTGTCGGACCGACTTGGGACAAGCGGAGGTATTCGCTGCGCACCTCGCCGAGCGA 3038 *F Query: 1114 TTTCAACCATTCAAGCTTGCCAGCCTGCAACAGGTTGAAGAAACTCAGGACCAGCTGAAC 1173 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3039 TTTCAACCATTCAAGCTTGCCAGCCTGCAACAGGTTGAAGAAACTCAGGACCAGCTGAAC 3098 *F Query: 1174 CAAGCGCTTCAAATGGATATGCCAATCACGCCGTTTGAACCCTGCGAGGTAGCCGAAGTC 1233 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3099 CAAGCGCTTCAAATGGATATGCCAATCACGCCGTTTGAACCCTGCGAGGTAGCCGAAGTC 3158 *F Query: 1234 ATTGTGCGCCAGAGTAACAACAAAGCACCTGGACATGACGTCATCTGCAACGCCACATTG 1293 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3159 ATTGTGCGCCAGAGTAACAACAAAGCACCTGGACATGACGTCATCTGCAACGCCACATTG 3218 *F Query: 1294 AAGGCCCTGCCCAGACAAGCGATCCTCTACATAACGTTGGTTTTCAACGCTATTGTGAGG 1353 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3219 AAGGCCCTGCCCAGACAAGCGATCCTCTACATAACGTTGGTTTTCAACGCTATTGTGAGG 3278 *F Query: 1354 TTGCAATACTTCCCTTATCAGTGGAAGCTCGGGATAATCTCCATGATCCACAAACCTGGC 1413 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3279 TTGCAATACTTCCCTTATCAGTGGAAGCTCGGGATAATCTCCATGATCCACAAACCTGGC 3338 *F Query: 1414 AAGCCGGAAAGGGAGCCCGCCTCCTACCGGCCGATCAGTCTCCTCCCTTCAATTTCGAAG 1473 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3339 AAGCCGGAAAGGGAGCCCGCCTCCTACCGGCCGATCAGTCTCCTCCCTTCAATTTCGAAG 3398 *F Query: 1474 GTGTTTGAGAGATTGATTGCTGTCCGGATTGTAAGCATTATGGAAGCCCAGGGGATTACC 1533 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3399 GTGTTTGAGAGACTGATTGCTGTCCGGATTGTAAGCATTATGGAAGCCCAGGGGATTACC 3458 *F Query: 1534 CCTGAGCACCAGTTCGGTTTCCGTGCTGGCCACTGTACTGTCGAGCAGCTCCATCGAGTC 1593 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3459 CCTGAGCACCAGTTCGGTTTCCGTGCTGGCCACTGTACTGTCGAGCAGCTCCATCGAGTC 3518 *F Query: 1594 GTCGAGCAAATTCTGACTGCCTACGACAGTAAGGAATATTGTAACAGCCTCTTCTTGGAC 1653 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3519 GTCGAGCAAATTCTGACTGCCTACGACAGTAAGGAATATTGTAACAGCCTCTTCTTGGAC 3578 *F Query: 1654 ATTCGAGAAGCGTTTGATCGAGTGTGGCACATTGGACTCCAACTGAAAATCAAGCAGACG 1713 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3579 ATTCGAGAAGCGTTTGATCGAGTGTGGCACATTGGACTCCAACTGAAAATCAAGCAGACG 3638 *F Query: 1714 CTGCCTGCTCCATATTTTGGGTTGCTGAAATCGTACCTGGAAGGAAGGAGGTTCGCTGTG 1773 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3639 CTGCCTGCCCCATATTTTGGGTTGCTGAAATCGTACCTGGAAGGAAGGAGGTTCGCTGTG 3698 *F Query: 1774 CGCTTTCATTCAGCAATTTCCACCGAGCACAACGTGGCAGCTGGTGTTCCACAAGGTAGT 1833 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3699 CGCTTTCATTCAGCAATTTCCACCGAGCACAACGTGGCAGCTGGTGTTCCACAAGGTAGT 3758 *F Query: 1834 GTCCTCGGCCCCCTGCTCTACTGCCTGTATAGCCACGACATGCCGCAGCCAGATGTAAGC 1893 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3759 GTCCTCGGCCCCCTGCTCTACTGCCTGTATAGCCACGACATGCCGCAGCCAGATGTAAGC 3818 *F Query: 1894 CTTTACGGGAAATCTATGCTGGCCACATTTGCCGATGACGTGTGCGTCACCTACAGGTCC 1953 *F |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3819 CTTTACGGGAAATCTATGTTGGCCACATTTGCCGATGACGTGTGCGTCACCTACAGGTCC 3878 *F Query: 1954 CGATGCGAGCACGACGCAGCCGATGGTATCCAGGACTTTGCATACCGGTTCTCGGAATGG 2013 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3879 CGATGCGAGCACGACGCAGCCGATGGTATCCAGGACTTTGCATACCGGTTCTCGGAATGG 3938 *F Query: 2014 GCAAGACGATGGAATATTGGCATCAATAGCAGTAAATCCAACAACGTCTGCTTCACTTTA 2073 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3939 GCAAGACGATGGAATATTGGCATCAATAGCAGTAAATCCAACAACGTCTGCTTCACTTTA 3998 *F Query: 2074 AAGCGGAGAACGCCACCGCCCGTCTACATCGAGGAAGTCCCCGTACCACAGCCGAACGCA 2133 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3999 AAGCGGAGAACGCCACCGCCCGTCTACATCGAGGAAGTCCCCGTACCACAGCCGAACGCA 4058 *F Query: 2134 GCAAAGTACCTTGGAGTGCTTTTGGATCGCAGACTCACATTTTCCAAGCATGTGACCGAC 2193 *F ||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||| *F Sbjct: 4059 GCAAAGTACCTTGGAGTGCTTCTGGATCGCAGACTCACATTTTCCAAGCATGTGACCGAC 4118 *F Query: 2194 ATCAGAACGCGCCTACGTGCTAAGGTGGCGAAGCACTACTGGCTACTTTCTTCGCGCAGT 2253 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4119 ATCAGAACGCGCCTACGTGCTAAGGTGGCGAAGCACTACTGGCTACTTTCTTCGCGCAGT 4178 *F Query: 2254 AAATTGTCGCTATCCAACAAGCTGACAATTTACAAACAGATCCTAGCACCAAACTGGAAG 2313 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4179 AAATTGTCGCTATCCAACAAGCTGACAATTTACAAACAGATCCTAGCACCAAACTGGAAG 4238 *F Query: 2314 TATGGGTGCCAAATCTGGGGCTTAGCCTGCGACAGCCACATCAAAAGGATCCAGGCTATT 2373 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4239 TATGGGTGCCAAATCTGGGGCTTAGCCTGCGACAGCCACATCAAAAGGATCCAGGCTATT 4298 *F Query: 2374 CAAAATAAGGTAGCAAGACTCATCACCGGGCTGCGAGTGGTTTGTTCGAAACACCACCCT 2433 *F ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 4299 CAAAATAAGGTAGCAAGACTCATCACCGG-CTGCGAGTGGTTTGTTCGAAACACCACCCT 4357 *F Query: 2434 GCACAGAGACCTGAAACTCGCAACGGTATTTGACGAAATAAACAAGCACTCGAGCAGATA 2493 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4358 GCACAGAGACCTGAAACTCGCAACGGTATTTGACGAAATAAACAAGCACTCGAGCAGATA 4417 *F Query: 2494 CCATGACAGGCTGGAGCGCCACAGAAATCGGCTGGCCAGCGCTTTAAACAGATCTCGCCC 2553 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4418 CCATGACAGGCTGGAGCGCCACAGAAATCGGCTGGCCAGCGCTTTAAACAGATCTCGCCC 4477 *F Query: 2554 ACCAAGGAGGCTCAATAGAAGGCAACCGAGGGATCTCATTACCCGATCTCCTTTGACAAG 2613 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4478 ACCAAGGAGGCTCAATAGAAGGCAACCGAGGGATCTCATTACCCGATCTCCTTTGACAAG 4537 *F Query: 2614 GGTCCGCAGAAGCTGACGCTTATCTTAAATCCTATTTGTTATATATGATTGTTATGTAAT 2673 *F |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 4538 GGTCCGCAGAAGCTGACGCTTATCTTAAATCCTATTTGTTATATGTGATTGTTATGTAAT 4597 *F Query: 2674 TGTAGTTAAATTACTGTAAATTTGAAAAAGCTAACTATAGTTAGCCGGCGAGCCCAAATG 2733 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4598 TGTAGTTAAATTACTGTAAATTTGAAAAAGCTAACTATAGTTAGCCGGCGAGCCCAAATG 4657 *F Query: 2734 GGCTGAATTAATAGATAAGAAGGACACAAAGGGGATTCAAGA 2775 *F |||||||||||||||||||||||||||||||||| ||||||| *F Sbjct: 4658 GGCTGAATTAATAGATAAGAAGGACACAAAGGGGCTTCAAGA 4699 *F Score = 3082 (468.5 bits), Expect = 0.0, Sum P(2) = 0.0 *F Identities = 622/626 (99%), Positives = 622/626 (99%), Strand = Plus / Plus *F Query: 16 GAAGT-GCCTGAAGTAGAGTGCTTCGTGCGACGTCACGAAATCGATGTATTACTGCTCAG 74 *F ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1874 GAAGTTGCCTGAAGTAGAGTGCTTCGTGCGACGTCACGAAATCGATGTATTACTGCTCAG 1933 *F Query: 75 CGAGACACACTGCAAGGGGGCAGAGACGCCTAAGCTATTCGGATTTGTAGCCTACACTGC 134 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1934 CGAGACACACTGCAAGGGGGCAGAGACGCCTAAGCTATTCGGATTTGTAGCCTACACTGC 1993 *F Query: 135 CAATGATCCGAGTGGTGGCAACGCCAAAGGCGGAGCAGCTATCTTAATCAAAAATAGCCT 194 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1994 CAATGATCCGAGTGGTGGCAACGCCAAAGGCGGAGCAGCTATCTTAATCAAAAATAGCCT 2053 *F Query: 195 TGCCCACTTTCCGCTAACACCAATAGCCACTGCCAAGGTGCAACTTGCGCCGGCGGTTAT 254 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2054 TGCCCACTTTCCGCTAACACCAATAGCCACTGCCAAGGTGCAACTTGCGCCGGCGGTTAT 2113 *F Query: 255 TGAAACGGCACTTGGTCCTATAAGCTTTGGAGCGGTCTACTGCCCACCGAGATTTGCATG 314 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2114 TGAAACGGCACTTGGTCCTATAAGCTTTGGAGCGGTCTACTGCCCACCGAGATTTGCATG 2173 *F Query: 315 GACTACGGACGAGTTTAAGGACATTTTGGAAGAGTTCCAGACGAAGTTCATTGTTGCAGG 374 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2174 GACTACGGACGAGTTTAAGGACATTTTGGAAGAGTTCCAGACGAAGTTCATTGTTGCAGG 2233 *F Query: 375 CGATTGGAACGCGTCCCACTGGCTCTGGGGTGCGGGAAGGAGCAACCAAAGAGGCATTGC 434 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2234 CGATTGGAACGCGTCCCACTGGCTCTGGGGTGCGGGAAGGAGCAACCAAAGAGGCATTGC 2293 *F Query: 435 ATTAGCGAATCTCGTCCTAAATTCGGAGGTGGACTCGCTAGCAACAGGAGGACCAACAAG 494 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2294 ATTAGCGAATCTCGTCCTAAATTCGGAGGTGGACTCGCTAGCAACAGGAGGACCAACAAG 2353 *F Query: 495 ATACCCGTACGGCTGTAGAGGCTCACCAGGGTACATCGATTTTGCACTGACAAAGGGTGT 554 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2354 ATACCCGTACGGCTGTAGAGGCTCACCAGGGTACATCGATTTTGCACTGACAAAGGGTGT 2413 *F Query: 555 GCTGGGCATCCACGCTAACATAAGTGCGGTTGTTGAGCTTAGCTCCGACCACCTGCCTCT 614 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2414 GCTGGGCATCCACGCTAACATAAGTGCGGTTGTTGAGCTTAGCTCCGACCACCTGCCTCT 2473 *F Query: 615 GGTAATTACGCTGGATGCGTCG-CAA 639 *F ||||||||||||||||||| | ||| *F Sbjct: 2474 GGTAATTACGCTGGATGCGGGGGCAA 2499 # *U FBrf0137466 *a Peifer *b M. *t 2001.8.3 *T personal communication to FlyBase *u FlyBase error report for CG12792 on Fri Aug 3 07:28:42 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 3 Aug 2001 07:28:43 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG12792 on Fri Aug 3 07:28:42 2001 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG12792 *F Release: 1 *F Gene annotation error *F P element l(2)k08138 hits gene CG12792. *F Comments: Hi there, *F I have been looking at P-element insertions in 41. One of these is *F l(2)k08138. I noticed that the sequence derived from this P matches *F essentially perfectly the sequence of CG12792, suggesting that it is an allele *F of it; this is also consistent with the cytology. As CG12792 is currently *F listed in Flybase as having no available alleles, it seemed like this might be *F worth noting if my thinking is correct. *F Mark *F Mark Peifer *F Associate Professor of Biology *F peifer@unc.edu *F http://www.bio.unc.edu/faculty/peifer/ *F Mailing address: *F CB#3280; Coker Hall *F Dept. of Biology *F University of North Carolina at Chapel Hill *F Chapel Hill NC 27599-3280 # *U FBrf0137467 *a Peifer *b M. *t 2001.8.13 *T personal communication to FlyBase *u FlyBase error report for CG12792 on Mon Aug 13 12:10:49 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 13 Aug 2001 12:10:49 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG12792 on Mon Aug 13 12:10:49 2001 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG12792 *F Release: 1 *F Gene annotation error *F P element l(2)09851 hits gene CG12792. *F Comments: Hi there, *F I have been looking at P-element insertions in 41. One of these is l(2)09851. *F I noticed that the sequence derived from this P matches essentially perfectly *F the sequence of CG12792, suggesting that it is an allele of it; this is also *F consistent with the cytology. I had sent a message last week that l(2)k08138 *F also matched this CG's sequence-- strangely, the 2 P's are reported to *F complement, though the end sequences of the two overlap. As CG12792 is *F currently listed in Flybase as having no available alleles, it seemed like *F this might be worth noting if my thinking is correct. *F Mark *F Mark Peifer *F Associate Professor of Biology *F peifer@unc.edu *F http://www.bio.unc.edu/faculty/peifer/ *F Mailing address: *F CB#3280; Coker Hall *F Dept. of Biology *F University of North Carolina at Chapel Hill *F Chapel Hill NC 27599-3280 *F 919-962-2271 (o) 919-962-2272 (l) *F 919-962-1625 (Department FAX) # *U FBrf0137468 *a Slack *b C. *t 2001.8.7 *T personal communication to FlyBase *u FlyBase error report for CG9469 on Tue Aug 7 04:43:59 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 7 Aug 2001 04:43:59 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: cathy.slack@ic.ac.uk *F Subject: FlyBase error report for CG9469 on Tue Aug 7 04:43:59 2001 *F Error report from Cathy Slack (cathy.slack@ic.ac.uk) *F Gene or accession: CG9469 *F Release: 2 *F Gene annotation error *F Gene CG9469 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F ATGATAATTCGTATTCCTTCGATTGCAGGCGCCTCGAAGGAGATCGGCACCGCCCTAACCCGCGTTTGCCTCCGCCACA *F AGGCGGTGGAGACGCGTCTGAAGACCTTCACCAGCACCATTATGGATTGCCTGGTGCAGCCGCTGCAGGAGAGGATCGA *F GGACTGGAAGCGCACGGTGGCCACCATCGACAAAGACCATGCCAAAGAGTATAAGCGCTGTCGCAGTGAACTGAAGAAG *F CGTTCCAGCGACACACTGCGCCTGCAGAAGAAGGCGCGCAAGGGTCAGACGGACGGATTGCAGTCCCTGATGGACTCGC *F ACATGCAGGATGTTACCCTGCGCCGGGCAGAATTGGAGGAAGTCGAGAAGAAATCCTTGAGGGCGGCCATGGTGGAGGA *F GCGTCTGCGCTACTGCAGCTTCGTCCACATGCTTCAGCCAGTGGTGCACGAGGAGTGCGAGGTCATGTCAGAGTTGGGT *F CACCTACAGGAAGCCATGCAGTCAATTGCCCTAGTAACCAAGGAGCCTAGTGTTCTGCCCCAGGCCTCCGAGGAGCTTA *F TCCATGACGCTAAGGCCAGCATTAATCTGTACCCGGAGTCCCCGGGTGGCGGTTCCGGCTCGCAGGGCGGCGGCTGCTC *F CAACTCGCTGGGTTCCCGAAAGAGCTCCGTCTGCTCCATCAGCAGTATGAACAGCAGCGGCTCGAGCAACTCGCCCGGT *F CACCATCACTATCCGCGCTCCCTTTCGCAGACTTCGAATGCAACGAACCAGACGGCAAATGTCTCCACCTGGCCCCCAC *F ATTCCCAGGATGGCGTCGACACCCTGCCACCGACCGCTGACCGTCCGCACACGATTTCGACGGCATACGAAAAGGGTCA *F CCAGCGTCCGCCGCTGACCGTCTACACGTTCCAAAACCCGGAGACCATTCACGAGTCGGGCAGCTGCCTGAACAACGGA *F ACAGCAGCCCCGAATGGACAGCCGTTATCCGGACAAGCCAC! *F TCCGGCCACCCAGAAATCACCGGCTGCCTCACTTAGCCGGCCTCCTTTGCCAGTTCGCTGCTCGTCGTTGGAGCGACCC *F CTTTCGGCCCAGAGTAACCACCGCCAGGGTAGCGGGAATAACCTGCTGCAGCGCCAGTGCCCCTCGCCGATTCCGGCTC *F ATATCACGAAAGAGCTGTCCGCAGCGCATCATGCACAGCAGCAGCAGCAGCAGCAAAATCAGCAGCCCCAGACGCCGCC *F CACCTATGTGAACATGTCGGAGCTGGCCACCATGGCCGCTTTGAAGCAGACCAATCAGCAGCAGAAGCCCTCTACGCCG *F CCTCTGCAGCAGCAGAGCTCCATTGACTCGACCAGCTCCCAGCATTCCAACGACTCCACCGGCTCTCATCAGCTCCTCC *F AGCAGCAGCAGCAGCATCATCAATCGCAGCAGAATCACCACTCAGCCACTGCCACACGCTCCCATTCCATATCCTCGAC *F GGCCTCGTCACTTCACTCGCATCCGTCGATCGACTCCACCGTCGCTTGCGGCTCGCTGGTGGGCCAGCACAACCACAGC *F ACCAGCACCAACACGAACACCACCTCGCCGTCCAGTGGCAGCTCCACGCCACAGAACCATTACTCGCCCCTGCTAACCA *F ACTCCCCCACGTCCACTGCCGCAGGTACTCCCAGTGGCAGCAGCTTAGGTCCTGGGTCCGGTTTGGGATTTGTCTACCA *F GGTCAGCTCCCCGACGCCTCCCTCCAGCGAGGTGCTGAAAATCACCGAGCAAGCCGCTGCAGGACAGGATCAGGGTCCA *F GCCAACAGCGTAGCGGACGAGACGGATGAGCGATCAAGGGCCTCAGTCCTGCAGAAGGCTTCAATGTTCGAAAAGGCGG *F CAGCAGCGGCAGCGGTATCGCCCCCAGCTCCCATGCAGATTGCATCCGGTTCCCCAGCTTCGGGAGGCGGAACTCGACG *F ATCCGAGGCGGAGCAGCAGGAAATGGACAAGTCTTTCGAAG! *F ATTCAATACAAGCACTAAATAATTTAATTGGCGAACTAGACTCGTTCCAACGCGAGATCGATG! *F AGGGCAAGGTCAAACCGCCGAGCAACATCATAAGCGGCAGCACCACCAGTAGCAACAACAACAATACGACGACCAGCAG *F CATCAGCAGCAGCGACAACAACAACCTGCCCGCCACCAGCAACATCGAGCCATGCGCCATCAGCAATCAGACGAACTCG *F AGCGGCTGCGGCACGGACATATCCGACACCACGTCCGACGAACTGGCCGGCGACGATATGGACGTCAGGCGGCGGGATC *F GGGATCGGGACCGGGATCTGCTGGGCGCCAGCGATTCGGAGCTGAGTCGCTGCTATGTGAGCGAGACGAGTTCGCTGAC *F CGGTGGCCTAACGGCCGGCGGCTACGAGAATCCCACTTTCGCGCACTTTGCGGCCAATGCCAATCGGGAGGACGCTGTT *F TCGCTGGCCTCCGACAGCGTTTGTCTCGGCCAGCCACGCCACGCCTATGTGGATACCTGCAGCGACAGCGGCAGTGCCG *F TGGTGGTGATCTACGACCACCAGATTCCCAACACACCCGACATTGAGTTCGTGAAGCAGAACTCTGAGATTGTAGTGCT *F GCGGACTAAGGATCCGCAGCCCCACGCGCTGCAGCTGCACGAGATGCGCGAGCTGCAGCAGTTGCCCGCCAATTTGGCC *F GGTTCGCCGGACTCCTCGCCGGACTCTGCCGGTGGCCAGGCACCGCCAACAGCAACTGTGGCGCCCGCCAAGCAGCGAC *F TCTCCTCGTTTCGCGCCACCAGTGAGCAGCAGTTGCAGCTCCTCGGACGCGGTAGCCCGCAAAGAGGTAAAACACCCAG *F TGAGCAGGCGGTACAAAGCAGGCCACAGGACCAGCATTTTCCACAGACACAACAGCAGGATATTGATGGCAGTAGTCCA *F CCAGTAGAACTTGCAAGGCGCCAGCTGCCCCCCAAGCCCACCAGTTTGAGTATTTTTAACGGCCCCGTGCCCACTGCGG *F GCGATAGGCCTGTCGTGCCGCGAAAGTCGGACTTTAAGGCT! *F GATCTAGATGCTAAAATACGCAGGCAAAAGCAGAAGGTTAAACAGCAGTTGCAGCAGCAGCAGCAGCAACAGCAGCAAG *F AGCAGCAAGAAACGCAGCAGCAGCAGCAAGCACCACAAGAACAGCAACACTCACCACAGTCGCCCCAAACCAGAAACTG *F TAATGTCACTAATCAACAAGCCGCCAATATTACTGCATCAGCATCTGCATTTGCAACCGCAACAGCATCCACAGACCCG *F TACCCGAATCCAAATCATAGAATGCCAAACCAAAATCAGACAGCCACATCCAATCACACGCAGTGCAAGACGCCCACAA *F TGGCATTGTCACCGTCATCACCTCGCGGCCATTTGCCATTATCATCGTCATCGCTATCGTCATTACCATTACCAGCCAC *F CACTTCATCACCATCAAATGCCCGGCCATCGATGTTGCCCGCCAGTGACCGACCACCCGCCCATCCATATGTGTGCTCC *F AATGCCCCAGCCAATCCCCACCACGCCAATAGCATTTCCAATGCCAATGCCCATCTCAAGCCGTGCATTACGCCCCGGC *F CGGCTTCTTTGTCGGGAGGAGCAGCCGGCGGTGGCTCCACGCGCATCGGGCGTCGATCGTCCATTAATCAGTCCAAGCC *F ACCGCCGCCAGTCAGACGCAGTTCGTCGGTGACCCCCAGTCCCAATGCCTCGGTCGGGCTGCAGCAGCAACCACAGCAC *F GCGACTCTGTCGCAGCAGAATCACCAACTAAGCAGCTCCAGCGAGCACTTACCGCCGCCACCGCCATTCATGCTGGACG *F CCATGCCCCAGATTCCCAGCTCAGCGCTGAAGGTATCGGAGACGGTAAGAGCCCTGGCAGCCATGCGGCACCAGCCAGC *F ATCACCTGTGTCCCTCAGACGCATGCAGCAGCAACAGCAGCAGCAGCTTCAGCAGCAACAGCAGCAACAGGTGCAGCAG *F CAGCAACCCCTATTGCAGTCTGCGCACAACTCCCCCCTCAA! *F AGAGGACCTGACCGTGTACTACGACTCCTACTTGGATCTGCACGCCTATGCCCAGGCCTTGGC! *F CAGCGGCCAACAGCCCGGCGGTCAGCAGATGGCCAACCAGCAACGCTTTACCCTCCAGCAGCAACATCAGCATCTGCAT *F CAGCCACAGCAACCGCCTGTCTACCAAGTCGATGCCACGTTCCGCACCTCATCACCAGCCGCGGGCGGAGGGGGTGGCG *F GCGGCATATACGCCCAGCCCAAACTGGTCAACAGCATGTCCAGCTTCCGCACCAGCAGCCCCAGTCCCAACGGACATGC *F TCACCCACTGCCACCGACACAGCCAAAGGCGAATCCGAACCTAATTGCACAGCTCAATGCACGACTCAGCGGCAAACAG *F CAGCAGCACCAACAGCAGCAGCATATCGAGGGGATCTACGGCAACCAGCAGGCGCCCGGAGGAGAGTCGATCTACATGC *F GGAGTGGCCTGTCCATGTCGCAGCCGCAACAGCAGCAACACTTTGACGGTAAATCTGAACAAATCCAGCTGCAGCACCA *F GCAACAGCATAGAATTTACGCTAGTTTCGGCACCTCATCATCAGCAATGTCATCCGCTCATGCGGCCAACAGCAGCACT *F AAACCGTCCATTCTAACACCGACCACCTCTTTCAATGCATTGCCTCACTTCCCCCTGTCTTCATCCACATCATCGTTGC *F TCTCCAAAGTCAGCTCATTCTCGAACTCTTCATCCGCATCCCCACCGACAACGGCAGCGACCTCTGGCTCGGCCAGTTC *F GCATTATCAGCCACCTCAGCCGCCGAATGCAGCAGTTGCTAACAGCAAAGACATGGCCATCTACTCAAGTTCGTTTACC *F AAAAATCCAGCAGCTGCGCAATCGCCGAACATGAGACAGGCTCATTCCCATCAGCACCACCAGCCGCCGCAGCAGCAGC *F ACTACACCTGTCCGCCTCCTCTGGAGGATCCCCCACCGCCGCCCATTTACGCCGCCGGTGCATCGGCCACGATGCCCAA *F GAAGATGGCCCGTCCGCCCACTGGCCAGAACGCGACTCACT! *F CGAGCGCCTATGCAGCAGCCTCGTCTACGGCCACGCTGCCCAAGAATATGATGCAGCAGCAGCAGCGGTTGCAGCACCA *F GCAGCAGTATCAACAGCCGGCAGGCATGGGCATTGGCAACGGCAATGGGCACCTAGGTCAGCGTCCGCAGTTGCCGCTG *F CCCCAGCAGAAGCTTAGAGCTGCACAGCAGCAGCACTTGGCGGAGCAGCAGCATCAGCAACAGCAAGAACAGCAGCAGC *F ACCACCAGCAGCACCAACAACACCAGCAACGCCAGCCGCCCATTCCTTCACGCCACTCAAGTGTGCAGCAAAAGATATT *F CGTATCAACGAATCCATTCATACAGACAACGGCCGTCAAGTTTCACTCGCCCTCGGCCTCGCCCACGTGCGGCTCGCCC *F GTTACTGGGTCCTTGGCTAGCATTTATGCCACAACCTCGCGTGGCGGCCACCATCACCAGCAGCAGCAGGCTCAGCAGC *F AGCAGCACTACTATCGCGATGCTGCCGGGGGCAACAGCAACGGCGGCGCTGCCTACTATAACCACAATGCCCATGCCCA *F TTCCCAGGCACATCATCCAAACTGGCAGTATTCGTGCCAAGACCAAGGCCGAATTCCTCGAGAATCTCAACGCGAAGCT *F GGCGAAGCAGGGAATGTCTGGACGAGCATTTGCCGTGCGAAATCTCATCAACAGCAAGGCCCTGATGTATCAAAATCCG *F CAAAAACTATCGCGACCCAGTGCGCAATACCGTAGACCACCCACCTATCCCAACACCAGCACAACCACCAATGCCACTT *F GCGAAGATCAGTGCTAA *F Comments: A partial cDNA clone of CG9469 was identified in a drosophila 0-24 *F hour embryonic cDNA library. This clone was found to contain an extra 50 bases *F due to the misannotation of an intron/exon within the CG9469 mRNA. # *U FBrf0137469 *a Eid *b J.P. *t 2001.8.5 *T personal communication to FlyBase *u FlyBase error report for CG9126 on Sun Aug 5 23:43:44 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 5 Aug 2001 23:43:45 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: J.Eid@anatomy.unimelb.edu.au *F Subject: FlyBase error report for CG9126 on Sun Aug 5 23:43:44 2001 *F Error report from Jean-Pierre Eid (J.Eid@anatomy.unimelb.edu.au) *F Gene or accession: CG9126 *F Release: 1 *F Gene annotation error *F Gene CG9126 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >gi|15042567|gb|AF328906.1|AF32890615042567 *F GTTTTAGGGATATCATCAGTTGAAAACTATGACTTTCGCGAGCAACAAATTTGATTTGTAAACTTATAAGAAACCGAGC *F CGATTTACCGATTTAAAGGAGACATATGCGATAATTTGTATTGAAGAAGTCCGCACACAATTCGTAACAATGCGAAAGA *F ATACCATTTGGAACTACTCTTTAATATTCTTCTGCTGTGTGCTGAAGAGCATAAGTACGCTAGATCATGGCCCGCACAC *F AGTATCAGTCGATTCGAATCGACACAACACACAGCATCAGTATAAGCAAAATCCCAATGTTGCCTCACAACGTCACTCA *F TCCCACGAATCTGGTCAGAGTTTACACAATTCGCAATCGGAACATGTCACCCATATTGCCGCATCGCACGCCGGAAGCG *F GCGGAGAGCACTCCACTCATTTGGCGCAAAATCTGCACAGGAGCTCATATAATCTTCTGAGCGAGGCCATGTCCCAGGC *F TGTCAGCAATGAATTTAGTTCCATGGGAAGTGGTTCAGCGGATGGAGCGTGTGCTGCTGATGATTTTGATTGCTACAGT *F GGAAGTGTTCAGGATCGCTTTGGCATGGAGGCTATTGCCAGCTTGCATCGTCAGCTAGATGATGACGATAATGGAAACA *F TCGATCTGAGCGAGTCCGATGACTTTTTGCGGGAGGAATTGAAGTACGACTCGGGCTACGAAAAGCGGCAGAAAGCGTT *F TCACTTCAATGACGATATGCATATATCGGTCAAAGAACTTTGGGAGGCCTGGCTCAGATCGGAGGTGCATAATTGGACC *F ATCGAGCAGACCACCGATTGGCTGGCTCAGTCCGTTCAGCTGCCGCAATACGTTGATCTGTTCAAATTACACAAGGTTA *F CTGGCGCTGCCTTGCCAAGATTGGCTGTGAATAATCTTCAGTATGTTGGCAATGTACTTGGCATCAAAGACCCTATACA *F CAAACAAAAAATCTCATTGAAGGCAATGGATGTGGTTCTGT! *F TTGGGCCACCGCGAGAAACTGGTACCCGCTGGAAAGACTACATATTGGTAACACTGTTGCTTAGTGCTATTATTGGTTG *F TTGGTACGCCTATCAGCAAAATAAGAATGCCAAACGGCATCTGCGTCGAATGGCCCAGGATATGGAGGGATTGCAGAGG *F GCTGAGCAAAGTCTACAGGAGATGCAGAAGGAACTAGAACGGGCCAGAATGGAGCAGGAAAATGTGGCAACAGAAAAAC *F TAGATTTGGAGCGTCGTCTAAAAGAAGCGCCCACTCTCAGTTCATCGAACTCGGATTTGGAAGTTCAGCAGCTGAAAAA *F GGAAATCGAGATGTTGCGCAACGAATTGTCCCGCGCCGAATTCGAGCTAGTAGACAACTGCTGGTCACCGCCGCCACAA *F CTGCAATCATGGCTTCAATACACATATGAACTAGAAAGTAAGAATCATCAGAAGAAGCGCACGTCGGCTGAGAAGCAGC *F TACAGTCGGCCAGAGAGGCTTGTGAGAAATTGCGTAAGAAACGGTCAAGTTTGGTGGGTGCGTTCGTTTCCACGCACGG *F AAAGAGTATTGATGATGTGGATCGGTCGATTGTTGAGGCACGGAATGCCCTCGGAGATGTAACAAACGAGCTGCAAGAA *F CGACTGCATCGCTGGAAGCAAATCGAGACGTGCCTTGGCTTAAACATTGTGAACAACAATGGTCTGCCCTACTTGGAGA *F ATGTTCTGTACGGTCGAAATGGGGGCTTACAAAGTTCCATGGGCATGAGTTCAACCAAGGGTTCTAGAGCACGTATTAC *F CAACAGCACCGAAGACCTGGACGATGAGTCCATACAAGGTAAGCTGAATTTTGAGAACTTTTCGCTGCTTGCCACGGAA *F TAAGGCTTGTGCGCTCGGTCTGTGAGGACAATAAAACCCAGCTCTTCAGAAAAGCATACTACTTGACGAACTAAATAAA *F ATTATAAAACCGCTTATTGAACCCTGAATGAAATCTGTTTT! *F ACATTAAGCTACTGTATTAATGAAGGTCATATAACATTATGTGTGTGTATGATATTTTAATCT! *F TTATATTTGTAATATATCCATTTTATTTTTGATGTATAAAAAAAAAAAAAAAAAAA *F Protein sequence: *F >gi|15042567|gb|AF328906.1|AF32890615042567 *F MRKNTIWNYSLIFFCCVLKSISTLDHGPHTVSVDSNRHNTQHQYKQNPNVASQRHSSHESGQSLHNSQSEHVTHIAASH *F AGSGGEHSTHLAQNLHRSSYNLLSEAMSQAVSNEFSSMGSGSADGACAADDFDCYSGSVQDRFGMEAIASLHRQLDDDD *F NGNIDLSESDDFLREELKYDSGYEKRQKAFHFNDDMHISVKELWEAWLRSEVHNWTIEQTTDWLAQSVQLPQYVDLFKL *F HKVTGAALPRLAVNNLQYVGNVLGIKDPIHKQKISLKAMDVVLFGPPRETGTRWKDYILVTLLLSAIIGCWYAYQQNKN *F AKRHLRRMAQDMEGLQRAEQSLQEMQKELERARMEQENVATEKLDLERRLKEAPTLSSSNSDLEVQQLKKEIEMLRNEL *F SRAEFELVDNCWSPPPQLQSWLQYTYELESKNHQKKRTSAEKQLQSAREACEKLRKKRSSLVGAFVSTHGKSIDDVDRS *F IVEARNALGDVTNELQERLHRWKQIETCLGLNIVNNNGLPYLENVLYGRNGGLQSSMGMSSTKGSRARITNSTEDLDDE *F SIQGKLNFENFSLLATE *F Comments: According to your annotation, the gene CG9126 has a transcript *F (CT26146) of 3,223 bp encoding for a protein 1,066 AA in length. *F We have published evidence to support that the 'stromal interaction *F molecule-like protein', designated D-STIM, is encoded by a transcript of 2,097 *F bp (cDNA), and is 570 AA in length. Biochem. J. 357 (3):673-685, (2001) *F The corrected sequence for D-STIM has been deposited in GenBank, Accession \#: *F AF328906. *F Thank you. # *U FBrf0137470 *a Millburn *b G. *t 2001.8.14 *T personal communication to FlyBase *u FlyBase error report for CG9126 on Tue Aug 14 05:38:36 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 14 Aug 2001 05:38:37 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG9126 on Tue Aug 14 05:38:36 2001 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG9126 *F Release: 1 *F Gene annotation error *F Gene CG9126 should be split. *F Comments: hello, *F The annotation CG9126 (AE003500; AAF48542) needs splitting in 2. *F Note all numbers refer to Release 1 sequences and annotations. *F The N-terminal portion (nucleotides 23 to 1695) of the CG9126 cDNA *F sequence matches 'Stim' (AF328906), described in Biochem. J. 357 *F (3):673-685, (2001). *F The C-terminal portion (nucleotides 1689 to 3219) of the CG9126 cDNA *F sequence matches the N-terminal half of 'Ranbp16' (AF222744). *F The annotation therefore needs splitting into 2 genes; Stim and *F Ranbp16. *F Note, that the 'Stim' (AF328906) and 'Ranbp16' (AF222744) sequences may *F overlap at the 3' end of Stim and 5' end of Ranbp16, although both *F transcripts are in the same direction. *F I also did BLASTP using the CG9126 protein sequence (Q9VXL6) at the *F NCBI and the best hits divide nicely into hits that cover either the *F N-terminal or C-terminal part of the CG9126 protein sequence and not *F both, providing further evidence that this annotation should be split *F in two. *F In addition, the CG9136 annotation matches part of the C-terminal *F portion of 'Ranbp16' (AF222744) and therefore needs merging with *F Ranbp16. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0137471 *a Peifer *b M. *t 2001.8.3 *T personal communication to FlyBase *u FlyBase error report for CG8325 on Fri Aug 3 12:05:09 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 3 Aug 2001 12:05:10 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG8325 on Fri Aug 3 12:05:09 2001 *F Error report from MARK PEIFER (peifer@unc.edu) *F Gene or accession: CG8325 *F Release: 1 *F Gene annotation error *F P element l(2)k15603 hits gene CG8325. *F Comments: This P's end sequence seems to be in the gene-- that P also appears *F to be allelic to l(2)k14710 and l(2)k15616 # *U FBrf0137472 *a Glaser *b R.L. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG12108 on Mon Jul 30 11:15:00 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 11:15:00 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: glaser@wadsworth.org *F Subject: FlyBase error report for CG12108 on Mon Jul 30 11:15:00 2001 *F Error report from Robert L. Glaser (glaser@wadsworth.org) *F Gene or accession: CG12108 *F Release: 1 *F Gene annotation error *F Gene CG12108 has incorrect exon/intron structure or translation start site. *F Comments: At the donor site of the third exon, near genomic region 8483690, *F where the sequence is TCAGGATGT. The T in TGT is currently included in the *F intron. If the T in TGT is instead included as exon sequence and the intron *F begins with GT, it shifts the reading frame such that the fly amino acid *F sequence more closely matches the human palmitoyl-protein thioesterase enzyme *F and a previously missing histidine residue of the conserved catalytic triad is *F then present in the sequence. # *U FBrf0137473 *a Artero *b R.D. *t 2001.7.23 *T personal communication to FlyBase *u FlyBase error report for CG5173 on Mon Jul 23 09:47:14 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Jul 23 17:48:42 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 23 Jul 2001 17:48:42 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 23 Jul 2001 09:47:14 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: r-artero@mskmail.mskcc.org *F Subject: FlyBase error report for CG5173 on Mon Jul 23 09:47:14 2001 *F Content-Length: 557 *F Error report from Ruben D. Artero (r-artero@ski.mskcc.org) *F Gene or accession: CG5173 *F Release: 1 *F Gene annotation error *F Genes CG5173 and CG4929 should be merged. *F Comments: Predicted genes CG5173 and CG4929 seem to be the same gene. At least *F three cDNAs match both predicted genes using ESts from both ends. In particular: *F GH11670-5', GH12157-5', GM01210-5' match CG4929 *F GM11670-3', GH12157-3', GM01210-3' match CG5173 *F Ruben D. Artero # *U FBrf0137474 *a Whitfield *b E. *t 2001.7.17 *T personal communication to FlyBase *u FlyBase error report for CG10239 on Tue Jul 17 03:26:41 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 17 Jul 2001 03:26:41 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10239 on Tue Jul 17 03:26:41 2001 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10239 *F Release: 2 *F Gene annotation error *F Gene CG10239 has incorrect exon/intron structure or translation start site. *F Comments: The translation in Celera is a C-terminal fragment, not full length. *F The protein kinase domain is truncated and does not carry the ATP-binding *F sites characteristic of a Tyr protein kinase. Adjacent protein CG10244 *F may carry the exon required for this protein. *F thanks # *U FBrf0137475 *a Eisberg *b A. *t 2001.7.11 *T personal communication to FlyBase *u FlyBase error report for CG9696 on Wed Jul 11 10:31:48 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 11 Jul 2001 10:31:48 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: ane2003@med.cornell.edu *F Subject: FlyBase error report for CG9696 on Wed Jul 11 10:31:48 2001 *F Error report from Andrew Eisberg (ane2003@med.cornell.edu) *F Gene or accession: CG9696 *F Release: 2 *F Gene annotation error *F Gene CG9696 has incorrect exon/intron structure or translation start site. *F Comments: The 3 prime EST LD32234 matches up to the intron between exons 12 *F and 13. There must be alternative splicing or an error in the exon assignments. # *U FBrf0137476 *a Ganguly *b A. *t 2001.7.12 *T personal communication to FlyBase *u FlyBase error report for CG8458 on Thu Jul 12 15:15:46 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 12 Jul 2001 15:15:47 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: Atish.Ganguly@umassmed.edu *F Subject: FlyBase error report for CG8458 on Thu Jul 12 15:15:46 2001 *F Error report from Atish Ganguly (Atish.Ganguly@umassmed.edu) *F Gene or accession: CG8458 *F Release: 1 *F Gene annotation error *F Gene CG8458 has incorrect exon/intron structure or translation start site. *F Protein sequence: *F MIFAITFFMGITSTLAAVLEPMSYYQYTQFQAPLSWEDITGKGLKQALDSCQQSFQWQRWNCPSQDFVQKNSKPEENSP *F NREDVYVAAISMAAIVHTLTKDCANGVIAGCGCTENALNVPCAHEPTKALEQYEKHFGSGSGAIGHNRRVVGALLQRSL *F EQECRCKQPGAVQGECQEEECVAVLKPFEAIAQDLLQMYDDAIQLEGASSNLKIMWQNIPLDSLVFMQDSPNYCERDAT *F GLWKGTRGRQCSKDGSGSLEERLSCQQLCRVCGYRVRSQHVRTERRCNCKLVWGFRLQCDVCVQLERQYSCY *F Comments: The 113 amino acid putative translation product of CG8458 reported *F here shows no homology to any Wnt family molecule and hence does not match *F the blast search results given. The 309 amino acid product appears to show the *F reported homology. # *U FBrf0137477 *a Stronach *b B. *t 2001.7.13 *T personal communication to FlyBase *u FlyBase error report for CG2272 on Fri Jul 13 11:24:26 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jul 13 19:24:29 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 13 Jul 2001 19:24:29 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 13 Jul 2001 11:24:27 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: bstronach@rascal.med.harvard.edu *F Subject: FlyBase error report for CG2272 on Fri Jul 13 11:24:26 2001 *F Content-Length: 6857 *F Error report from Beth Stronach (bstronach@rascal.med.harvard.edu) *F Gene or accession: CG2272 *F Release: 1 *F Gene annotation error *F Gene CG2272 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CAGCAACTGGTTTATGTGAGCCACAACAAAATGATCGACAAAACCTTGTTCTACCACAAATATTAACAATTACCAATA *F TA *F AAGGTATTTAAACAACTACCTTGATCACAATTTTAGTTAAAATTATGCCAACGAACTGGAACCAAAATCCCAGGTGAACC *F GCAAATGTAACCAGTTCACTCGGCTGGGTCCAAAGACCAGTTCAATTAAACTAGTTCACATTTGAACAGTGCAACTCTAG *F TTTTTTTTTTTTAATACATATGTGTTTAAAAAACATATGCAGAAACGGGCTAATAAAACGGATATAACCGATTGCACAGG *F ATCAAGGATAGATCAAAATAACCGACGACGTTAACGGCGACTGCGACTGCGAGAGAGCGACCATGGTGTGCAGCAGCGCG *F TGTGCGTGTGTGTGTGTTTTGTACCGTGTTGTCTGATTGTGTGCCGTTGTTGTTGTGATTGTTGTTCTCGCTGTTGCTAT *F TGTTTTTGCTCTTGTGCGTGTGGTCAACGGACGGACAGACGGACGGACAGTGGGACACAAGGAGAGTGGAGAATCCGAAT *F CCGGTGGGCAGGAGAGCAGGATTAGCCGCAACATCCTTACCCCGAAACAAAAGCAAGTTAAAAAGCGAAGGAAGCAAAGA *F GAAAACAACAAAAAAGGAAAAGAAGCAGAAAACAAAAACAAAGAAGAGATTCATGGTTTCCATGTCGCCAACTGTGCGCC *F TCTGATGCCCTCGCAGGTCTCCCGAGGATTGGACACCACCAACATGCTGCCGATCAGCGAGGAGCAGCAGCTGCAACAGC *F AGCAGCAGCAGCAGCAGTTAGAGCAACTGCATCATCCGCAGATACCAGAGATTCCCATACCAGATCTAGAGCAAGTCGAG *F ACCCAGGTGGGCGATGGCAGCCTGTGGACCGCGCTGTATGACTACGATGCCCAGGGCGAGGATGAGCTGACACTGCGACG *F CGGCGAAATCGTTGTGGTCCTGTCCACGGACAGCGAGGTGTCTGGCGATGTGGGCTGGTGGACCGGCAAGATTGGAGATA *F AGGTCGGTGTCTTTCCCAAGGACTTTGTCACCGATGAGGACCCACTGCAACTGAATGTCTCGTCCGCCATTGGCGACATC *F CAGCCGCATGAGATCGAATACAATGAGCTGGACATCAAGGAAGTTATCGGCTCCGGTGGCTTCTGCAAGGTCCATCGTGG *F CTACTACGATGGCGAGGAGGTGGCTATTAAGATTGCCCATCAAACGGGCGAGGATGATATGCAGAGGATGCGGGACAATG *F TCCTGCAGGAGGCCAAACTGTTTTGGGCCCTCAAGCACGAAAATATAGCAGCCCTGCGAGGAGTATGTCTGAATACCAAA *F CTCTGTCTGGTGATGGAGTATGCCAGGGGAGGAAGTCTCAATCGCATACTGGCTGGCAAGATACCACCCGATGTACTAGT *F CAACTGGGCCATTCAGATAGCCAGAGGCATGAACTATCTGCACAATGAGGCGCCCATGTCGATCATACATCGCGACCTCA *F AGTCCTCCAATGTGCTGATCTACGAGGCCATCGAGGGCAATCATCTGCAGCAGAAGACGCTCAAGATCACGGATTTCGGT *F TTGGCCAGGGAGATGTACAACACGCAGAGGATGAGTGCGGCGGGCACGTACGCTTGGATGCCACCGGAGGTCATCAGCGT *F TAGCACCTATTCCAAATTCTCCGATGTTTGGAGCTACGGTGTCTTGCTGTGGGAACTGATAACGGGCGAGACGCCCTACA *F AGGGCTTCGATCCGTTGTCCGTGGCCTATGGCGTGGCTGTTAACACGCTGACATTGCCCATACCAAAAACCTGCCCCGAG *F ACGTGGGGAGCCCTGATGAAGAGCTGCTGGCAAACGGATCCGCACAAGCGACCGGGCTTCAAGGAGATTCTCAAACAGCT *F GGAGAGCATCGCATGCTCCAAATTCACCCTAACGCCCCAGGAGTCGTTTCACTACATGCAGGAATGCTGGCGAAAGGAGA *F TCGCTGGAGTGCTACACGATTTGCGCGAAAAAGAAAAGGAACTGCGCAACAAGGAAGAGCAGCTGCTCAGGGTGCAAAAC *F GAGCAGCGCGAAAAGGCGAATCTCCTGAAGATCCGGGAGCAGAATCTGCGCGAGCGTGAAAGAGTGTTGATCGAACGGGA *F GCTTGTCATGTTGCAGCCGGTGCCATCGAAACGCAAACACAAAAAGGGCAAAAAGAACAAACCACTGCAGATATCGCTGC *F CCACGGGCTTCCGGCACACGATTACGGCTGTACGCGACAAGGCGGAACAGCCGGGATCACCTTCCTTCTCCGGATTGCGC *F ATCGTGGCACTGACCGATGGGCACAAGGGCAAGACGTGGGGCCCGTCGACGATGCACCAGCGGGAGCGATCCCTATTGCC *F TTCGCAACTGAGCGGCGGTCAGCCGGAATGGCCCGCCCAGACCTCAACTCACTCCTCATTCAGCAAGAGTGCTCCGAATC *F TGGACAAGAAGCAGCAGCAGCAGAACCAGCAACAGGTGGCCTCGCTGACACCGCCGCCGGGTTTGGGAATTTTGGGTGGA *F AGTGGAGGCGCTGGTGGGACGCCGGCCACACCATTGTTGTACCCAGGTATACCCATTATACTAACGCGCCCCAACAACAA *F CAACATTGGCAATTGTAAAGCCATCACCACCACCATCACAACTACCACCACCACAACCACCAACAACAACAACAACAACA *F ACAACAGCATCTCCGCCAACAACAACAATCAGCTAAATAATATTAGTACTATTAATAGCAATAACAATAACAATCAAACT *F AACCTGACCTCCCAACCGAACACGATAATTGTCCTCCAAAACGGCCGGAACAACTCCAACAGCAGCACCACCAGTCAATC *F CCCAGCCAAGATCTACCATCGCGCCAGGAGCCAGGAGTACGGACTTGATCATCCGCTTGCTTATCAGCCACCACCACTCT *F ATTTGGTAACGGATGACAGCTCGGAAACGGACACCGTTGCCAGTCCGACCGGTTGCTTTCACTTCCTGAAGTCCGGCAAT *F TCATCGGCGGCCAGTGGTGCCGTGCATCTGCATCGCTTTGGCGGCAGTCTGGGCAACAGTCCAGCTGTGGGCAGAAAGAA *F GCATTCGCTGGACAGCAGCAGTCATCATCCGCCTGCGAACGGTAGCAATAGCTTTGCACTGCCCAACCAGTTAACTCTGC *F CATCGGAGGATAACAATACGTATGACCATGCCTTCTATCGGGATGTGATCAAAAAGATGTCCATGGCCAGCAGCGAAAGG *F GTAAACTCCAAATCCAGTGGCGACCTGACCATGTACAACTCCAGCACACCGCTGACTGCCAGGGATTGCGATGATGCGGA *F GGAGGCCTTCGAGGGTGGACGCTTTCAGCGTAACTTTTCCGGCTCGCAGTTTCCACGGCACTGCTTCTTCACCAGGCAGG *F AGGAGGAAGGGGAAGCAGAGGATGAGGACGCGGTGGCGGCGGAGGTGGACACAGCAGATGCGGATGCCGACGATGAGTGT *F CAAGTGCCCGCCAGTCAGATGCGCCAGAACTCCACCACGTCGCGCAAGAGTTCAGTGACCTTCCAGAGCGTCAGTTTCGA *F AGAGCCCGATTTTGTGGCCACGCCGCGAACGACTGCCAGATCGGATCTATATACATCCAGTGCCTCAATTAGCTTCGCCA *F CCTACCGCTCCGCCTCGCCATCGCTGTCCTCATCGTCCACCACTGCGTCCGCCTCACCGTCCATTGCTTCTACGGAAGCC *F GTGAACGGCTATCACATGCAGGAGAACTCCATACTGAATACGCGTCGGATGCAAGATGTTCAGCCGCATCCCGATGTCAT *F TAAGCTGCGGGCCCAGGAACAGCGCCAGCAGACCAAGAACCAAAAGAAGCAACGGCCCAAGCACATAACCAAATCCAAGT *F CTGTTGAGGCGCCAGTCGAGGGACAGCACCATGAACATGATGACCACAATGATCCCCAGCACCAGCATCACTCGGCTGGA *F TCGAGCAAAATCCGTGCCCTTTTCAATCTCTTTACGCGCTCGCGAAAGAAGTACAGCAAGCTGGCGGAGCACAACATGGT *F CGGTGGACCCGAGTTCTGTGCCATTGATCCCTATCAAACCGATTTGGCGATGGGGGGCAGCAGTCGCTCTTTGAAGCGCA *F AAGGCAAAAAGCCGCAGACGCAATCCTGCGAGCAACTGGAGCGATGCTAAAGTCCCAGTTGTGCGCCGTGCTGCAGTGTT *F TGCTGTTGGGAGTTGGCCCGCCAGATGAGAACCGCCTAATTTAGCTCATTGTTTTTATATTAAAACTGTAGACTTAAGTC *F AGCACGCAAANGGAAGAGCCGAAGARNATGAAACAGCAGGCGNAGGATTAGCCATAGCTAATGTGTGAAATTAGTAGGGC *F CCAGAGATACAGATACCTATCCGAATTCTTTCCGTCTKAACGAAATCATAGAACTAAACAAAAAACCCCTCTGTCCGCGG *F TTGGCATTTCATAATAACCGAATTGTTCCTAACGAATGCAATATGTTGTGCGAAATTCACAAATCAAGTTAATCCCTAAT *F TAGTCCAGTGATTTGTTTTGTTTTTTTTTTTGTGCCAGAGGAGTGGCTAGAAAATTGCAATGTGTAGCACAGAAGTGAGG *F ACGAACTTTTGAGGATCAAACGGATAGATAGACTAACGCCGCTTGTACGTAGACAAACAGACACGGAGAGAAAAAACGTG *F TGCATGGGCCAATGGCAAAATATATTGAACTAAACCATATACATAAATGTGTAACAATTGGATTAACATTAAGCTATATT *F TATACCGATGCGTAAGTGCGTCCTTACACATTACACATTACACACATCTTAGTTGAACTAAGTTCTAGTTTTGTAGGAAC *F TCGAACATTTTTTTGGAGTCCTTCCTCCTTTTTTTTTCACCCCCTCAATCTTTGTAAGAGATATTTTCCAAAATTTCCAT *F CGCTTGCGGCATTTCGTTTGGCTTAAGTACACTTAACGAAAAGTTGCGGTGCACATTAATTTTAATTTGATATTCGACTT *F TTTTGGTATTTTGAGCACAGTTTTATTTACATACATTTATTATGCAATCGGGTTTTCCATTTTATTTATTTATGATTTAT *F TTCTACAAA *F Protein sequence: *F >MPSQVSRGLDTTNMLPISEEQQLQQQQQQQQLEQLHHPQIPEIPIPDLEQVETQVGDGSLWTALYDYDAQGEDELTLR *F RG *F EIVVVLSTDSEVSGDVGWWTGKIGDKVGVFPKDFVTDEDPLQLNVSSAIGDIQPHEIEYNELDIKEVIGSGGFCKVHRGY *F YDGEEVAIKIAHQTGEDDMQRMRDNVLQEAKLFWALKHENIAALRGVCLNTKLCLVMEYARGGSLNRILAGKIPPDVLVN *F WAIQIARGMNYLHNEAPMSIIHRDLKSSNVLIYEAIEGNHLQQKTLKITDFGLAREMYNTQRMSAAGTYAWMPPEVISVS *F TYSKFSDVWSYGVLLWELITGETPYKGFDPLSVAYGVAVNTLTLPIPKTCPETWGALMKSCWQTDPHKRPGFKEILKQLE *F SIACSKFTLTPQESFHYMQECWRKEIAGVLHDLREKEKELRNKEEQLLRVQNEQREKANLLKIREQNLRERERVLIEREL *F VMLQPVPSKRKHKKGKKNKPLQISLPTGFRHTITAVRDKAEQPGSPSFSGLRIVALTDGHKGKTWGPSTMHQRERSLLPS *F QLSGGQPEWPAQTSTHSSFSKSAPNLDKKQQQQNQQQVASLTPPPGLGILGGSGGAGGTPATPLLYPGIPIILTRPNNNN *F IGNCKAITTTITTTTTTTTNNNNNNNNSISANNNNQLNNISTINSNNNNNQTNLTSQPNTIIVLQNGRNNSNSSTTSQSP *F AKIYHRARSQEYGLDHPLAYQPPPLYLVTDDSSETDTVASPTGCFHFLKSGNSSAASGAVHLHRFGGSLGNSPAVGRKKH *F SLDSSSHHPPANGSNSFALPNQLTLPSEDNNTYDHAFYRDVIKKMSMASSERVNSKSSGDLTMYNSSTPLTARDCDDAEE *F AFEGGRFQRNFSGSQFPRHCFFTRQEEEGEAEDEDAVAAEVDTADADADDECQVPASQMRQNSTTSRKSSVTFQSVSFEE *F PDFVATPRTTARSDLYTSSASISFATYRSASPSLSSSSTTASASPSIASTEAVNGYHMQENSILNTRRMQDVQPHPDVIK *F LRAQEQRQQTKNQKKQRPKHITKSKSVEAPVEGQHHEHDDHNDPQHQHHSAGSSKIRALFNLFTRSRKKYSKLAEHNMVG *F GPEFCAIDPYQTDLAMGGSSRSLKRKGKKPQTQSCEQLERC *F Comments: This is the nucleotide sequence of EST clone GH26507 derived from *F the predicted locus, CG2272. *F The longest ORF is translated. *F Browser: Mozilla/4.0 (compatible; MSIE 5.0; Mac_PowerPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137478 *a Davis *b T. *t 2001.6.11 *T personal communication to FlyBase *u FlyBase error report for CG7689 on Mon Jun 11 03:54:42 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 11 Jun 2001 03:54:42 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@cardiff.ac.uk *F Subject: FlyBase error report for CG7689 on Mon Jun 11 03:54:42 2001 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: CG7689 *F Release: 1 *F Missed gene *F Comments: Additional annotations for gene CG7689 (fruitless FlyBase ID *F FBgn0004652) *F These are described in the two following references: *F Baker BS, Taylor BJ, Hall JC. Are complex behaviors specified by dedicated *F regulatory genes? Reasoning from Drosophila. Cell. 2001 v105: p13-24 *F Goodwin SF, Taylor BJ, Villella A, Foss M, Ryner LC, Baker BS, Hall JC. *F Aberrant splicing and altered spatial expression patterns in fruitless mutants *F of Drosophila melanogaster. Genetics. 2000 v154: p725-45 *F Sequences from complementary AE003722 *F There are two additional promoters *F Promoter P2 *F Bases 121152..121101 *F Start codon 121126..121124 *F aa seq MLQKNSVRK *F This is spliced to 50339 *F The only known 3’ end of this transcript is the type A Zn finger domain (gene *F cg7689) *F Promoter P3 (two exons) *F Bases 95010..94936 *F Bases 92940..92672 *F Start codon 92680..92678 *F aa seq MTR *F This is spliced to 50339 *F The only known 3 end of this transcript is the type E Zn finger domain (gene *F cg14307; also FBrf0136019) # *U FBrf0137479 *a Burnette *b J. *t 2001.5.23 *T personal communication to FlyBase *u FlyBase error report for CG6536 on Wed May 23 15:21:06 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 23 May 2001 15:21:06 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: jburnette@virginia.edu *F Subject: FlyBase error report for CG6536 on Wed May 23 15:21:06 2001 *F Error report from Jimmy Burnette (jburnette@virginia.edu) *F Gene or accession: CG6536 *F Release: 1 *F Assembly error *F Comments: I was designing pcr primers to CG6530 and noticed that the sequence *F between CG6530 and CG6536 is *F practically identicle. This includes the intronic region. The surrounding *F genomice *F sequence is different. It is not unlikely that they are duplications--most of *F the *F G-protein coupled receptors are found as pairs. But for all of the first *F intron and *F possiblely the second to be identical--even after a duplication event--is *F highly unlikely. # *U FBrf0137480 *a Levis *b R. *t 2001.6.8 *T personal communication to FlyBase *u FlyBase error report for CG7682 on Fri Jun 8 12:53:17 2001. *F From FlyBase-error@hedgehog.lbl.gov Fri Jun 08 20:53:30 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 8 Jun 2001 20:53:30 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 8 Jun 2001 12:53:17 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG7682 on Fri Jun 8 12:53:17 2001 *F Content-Length: 410 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG7682 *F Release: 1 *F Missed gene *F Comments: It appears to me that CG7682 probably corresponds to the gene spread *F (sprd). The semi-lethal P element insertion allele of spread, sprd05284, is *F inserted in what is predicted to be the 5'UTR of CG7682. *F Browser: Mozilla/4.75 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137481 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG15039 on Tue Jul 31 12:04:25 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jul 31 20:04:25 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 31 Jul 2001 20:04:25 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 12:04:26 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG15039 on Tue Jul 31 12:04:25 2001 *F Content-Length: 3414 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG15039 *F Release: 1 *F Gene annotation error *F Gene CG15039 has a mistake in the supporting evidence or functional assignment. *F Comments: CG15039 can be deleted from GadFly--it appears to be part of a *F Waldo-A-like element (just 80% *F identity, but I'm pretty confident this annotation is transposon *F sequence). *F Query= CG15039|FBgn0030957|CT34904|FBan0015039 GO: located on: X *F 17D5-17D5; |cDNA sequence *F (417 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 895 5.5e-53 *F 2 gb|AJ278684|pilger DME278684 5108bp Derived from AJ278684... 626 7.1e-25 *F 1 gb|AC005847|Waldo-B WALDOB 5180bp 510 1.3e-19 *F 1 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 895 (140.3 bits), Expect = 5.5e-53, Sum P(2) = 5.5e-53 *F Identities = 227/281 (80%), Positives = 227/281 (80%), Strand = Plus / Plus *F Query: 1 ATGCGCAGGTGTCCAAGGGACAAGTGCCCAAAGTTCTGGCGCTCAAACCGCACGACTGGT 60 *F | | ||| ||| | || || | |||||| | | || | || | | ||| *F Sbjct: 969 AGGTGCAAGTGCCAAAAATCCAGGCGCCCAA-GC-CGAGCAACCCTAC-GTTGGG-TGGC 1024 *F Query: 61 AGAGGAGAAGGGAAGGCGACATCCAGATCAAACGAGTCCAATACAGGCAGCTACGCATCT 120 *F | || |||| ||||| ||||||||| ||||||||||||| ||||||||||||||||| *F Sbjct: 1025 AACGGTGAAGAAAAGGCAACATCCAGACCAAACGAGTCCAAACCAGGCAGCTACGCATCT 1084 *F Query: 121 GTCGCCAAGAATGCTAGCACGAGTGCATATTGGACCAAGGTGAAGCCTATGCGCCCGCGT 180 *F ||||||||||||||||||||| ||| | | ||||||||||||||||||| ||||| |||| *F Sbjct: 1085 GTCGCCAAGAATGCTAGCACGGGTGTAGAGTGGACCAAGGTGAAGCCTACGCGCCTGCGT 1144 *F Query: 181 AAAAAGCCTGAGGCACGGATCGTGAAGAAGACGGGTGAGGCTTGGTACGCAGAGATGCTC 240 *F ||||| || ||||||| ||||| || ||||| || ||||||| ||||||||||||||| *F Sbjct: 1145 AAAAAACCGGAGGCACTCATCGTAAAAAAGACAGGAGAGGCTTCGTACGCAGAGATGCTT 1204 *F Query: 241 CGGAAACTAAGATCGGACCCGAGCCTTAGCGAACTGGGCAG 281 *F ||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 1205 CGGAAGCTAAGATCGGACCCGAGCCTTAGCGAACTGGGCAG 1245 *F Score = 431 (70.7 bits), Expect = 5.5e-53, Sum P(2) = 5.5e-53 *F Identities = 121/154 (78%), Positives = 121/154 (78%), Strand = Plus / Plus *F Query: 268 AGCGAACTGGGCAGAAGAATTCGGAGAACTCAGCTAGATGAGCTGTTGCTCGAAGTAGTG 327 *F ||| | | | || ||| ||| |||||||| ||| || ||||||||||||||| |||| | *F Sbjct: 1244 AGCCA-C-GTGC-GAAAAATCCGGAGAACGCAGAAAGGTGAGCTGTTGCTCGAGGTAGAG 1300 *F Query: 328 GGAAAAGCCTCGGGAAGCTTTCCCAAGTATAAAAGCGATCTAGAGGCGACGGTCAATGAT 387 *F || ||||| |||| |||| | ||||||| ||| ||||| || || ||| || ||||||| *F Sbjct: 1301 GGGAAAGCTTCGGAAAGCGTCCCCAAGTTTAAGAGCGACCTGGAAGCGGCGCTCAATGAC 1360 *F Query: 388 TTGGCATCTGTGCGG-CAG---CTCAAAGAATAG 417 *F ||||| |||||||| ||| | |||||||||| *F Sbjct: 1361 TTGGCCTCTGTGCGCACAGGAGCGCAAAGAATAG 1394 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137482 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG17411 on Tue Jul 31 12:08:55 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jul 31 20:08:55 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 31 Jul 2001 20:08:55 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 12:08:55 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG17411 on Tue Jul 31 12:08:55 2001 *F Content-Length: 5713 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG17411 *F Release: 1 *F Gene annotation error *F Gene CG17411 has a mistake in the supporting evidence or functional assignment. *F Comments: CG17411 can be deleted from GadFly--it appears to be part of an *F aurora element (just 67% *F identity, but again over the whole length, so likely to be transposon sequence). *F Query= CG17411|FBgn0040048|CT34126|FBan0017411 GO: located on: U; |cDNA *F sequence *F (973 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AB022762|aurora-element DMAURA 4263bp Derived from AB0... 1094 2.0e-76 *F 2 Tinker Tinker 6112bp 193 0.020 *F 1 *F >gb|AB022762|aurora-element DMAURA 4263bp Derived from AB022762 (d1268008) *F (Rel. 59, Last updated, Version 1). *F Length = 4263 *F Plus Strand HSPs: *F Score = 1094 (170.2 bits), Expect = 2.0e-76, Sum P(2) = 2.0e-76 *F Identities = 376/559 (67%), Positives = 376/559 (67%), Strand = Plus / Plus *F Query: 1 ATGTGCCACCAAAACGTGGAGGCCACCATCGGCGAGATTCGTCGAAAGTTTTGGATAACT 60 *F ||| || ||||| ||||| || || |||| ||||| || || ||| ||| | || *F Sbjct: 2929 ATGAAGCATCAAAATGTGGATGCTACGATCGCGGAGATCCGGACAATGTTCTGGGTCACA 2988 *F Query: 61 AACCTGAGGAGGCTACTACAAAAGGTGGTGGCTAATTGTAACGTATGCAAGTTGCGGAAG 120 *F || ||||| | | | | | || | | || |||| |||||||||| | *F Sbjct: 2989 AAGATGAGGCGTGTGATGCGGAGAGTCATCTCATCGTGCAACGAGTGCAAGTTGCAGCGA 3048 *F Query: 121 GCACGACCGACACAAACGGAGATGGG-CCCATTGCCGGAGAATCGCCTTGAGGCAAATGG 179 *F || || |||| | || ||||| ||| | ||||| | || || || ||| *F Sbjct: 3049 GCGCGGCCGATGCCGCCGATAATGGGACCCCAT-CCGGAAGACAAACTGGATGCGGGTGG 3107 *F Query: 180 ATGGCCATTTAAATTCACTGGCCTGGACTATTTCGGACCATTGCTTGTGTCGATTGGGCG 239 *F ||||||||| |||| ||| || |||||||| || || ||| |||| ||| | | || *F Sbjct: 3108 ATGGCCATTCAAATACACAGGACTGGACTACTTTGGGCCACTGCTGGTGACTGTGTCCCG 3167 *F Query: 240 CCAAACAGAGAAGAGGTGGGTGGCGCTATTCACCTGCCTAACTACGAGAGCCGTCCACTT 299 *F || | |||||| ||||| || | || || || | || || || || | ||| | *F Sbjct: 3168 TCACAAGGAGAAGCT-TGGGTCGCCTTGTTTACGTGTTTGACGACAAGGGCGATTCACCT 3226 *F Query: 300 GGAGATGGCTCATGATTTGTCAACGGATTCATGCATCATTGCTATGAGAAACTTCATGTG 359 *F |||| |||| ||||| |||| |||||||| ||||| ||||| || || |||||| | || *F Sbjct: 3227 GGAGCTGGCGCATGACCTGTCGACGGATTCCTGCATAATTGCGATCAGGAACTTCGTCTG 3286 *F Query: 360 CCGCCGCGGACCGGTAGTTAGGATAAGAAGCGATAATGGCAAGAACTTCGTGGGAGCCGA 419 *F ||| | || || ||| ||| | | |||||||| |||||||||||||||||||| || *F Sbjct: 3287 CCGTAGAGGGCCAGTATATAGACTGCGCAGCGATAACGGCAAGAACTTCGTGGGAGCTGA 3346 *F Query: 420 TAAAGAGGCCAGACGATTCAGCGATGTATTTGACTCAGCGAAGATACAGGGCGAGTTGTC 479 *F | || ||||| || || |||| ||||| || | |||| | ||| | |||||| | *F Sbjct: 3347 CAGGGAAGCCAGGCGCTTTGGCGACGTATTCGAGATGGAGAAGCTTCAGAGTGAGTTGAC 3406 *F Query: 480 ATCTA-AAGCAATCGAATGGATCTTTAACTGCCCATCAAACCAGTGCGTGAAGAAGGT-T 537 *F | | ||||| | |||||| | ||||| || ||| | |||| || | ||| | || | *F Sbjct: 3407 AAGCAGAAGCATT-GAATGGGTGTTTAATTGTCCAGCGAACCCGT-C-TGAGGGCGGAGT 3463 *F Query: 538 TTGGC-GCATACGATGAAG 555 *F |||| || | | || || *F Sbjct: 3464 TTGGGAGCGCATGGTGCAG 3482 *F Score = 748 (118.3 bits), Expect = 2.0e-76, Sum P(2) = 2.0e-76 *F Identities = 310/473 (65%), Positives = 310/473 (65%), Strand = Plus / Plus *F Query: 520 CAGTGCGTGAAGAAGGTTTTGGCGCATACGATGAAGGAACTTGCACCCAAGGAGCACGTG 579 *F |||||||| |||| || || ||||| ||||||| |||| || | ||| || || *F Sbjct: 3480 CAGTGCGTCAAGAGAGTACTGCGTCATACCCAGAAGGAAGTTGCGCCGAGGGACCATGTA 3539 *F Query: 580 CTGGAGAACTTGCTGATCGAGCCGGAGAAT--TC----C-CGC--CCGCTCACGTACTTG 630 *F |||||| || ||||| ||| |||||||| | | ||| |||||||| ||||| *F Sbjct: 3540 TTGGAGAGTTTCCTGATTGAGGCGGAGAATATTGTAAACTCGCGTCCGCTCACCCACTTG 3599 *F Query: 631 CCCGATCACAGTGGACCAGGAGGCTCCACTGACACCAAACGACCTGTTGAAAGGAATGCC 690 *F || | | |||||||||||||| || |||| |||||||| || | || ||| | | *F Sbjct: 3600 CCTGTGGAT-GTGGACCAGGAGGCGCCGTTGACGCCAAACGATCTTCTCAAGGGAGTAGC 3658 *F Query: 691 CGACGTGCCAAATCTTCCTGGAGATGATGAATTGGCATCTGAGAGATGCACAACAAGGAA 750 *F | | |||| || |||||| |||| | | || | | || || ||||| *F Sbjct: 3659 CAATCTGCCGGATACGCCTGGATTGGATGCGGAGCTGCCCAAGGAAGGTACTACGAGGAA 3718 *F Query: 751 ACAGTGGCGTATCGCTCGGATGATAAGAGATCGGTTCTGGAA-ACGATGGGTGCACG-AG 808 *F |||||| | || |||| || || |||| || ||||||| | | ||||| || | || *F Sbjct: 3719 GCAGTGGAGAATTTCTCGCCTGCTACGAGACCGTTTCTGGAGGAAG-TGGGT-CATGGAG 3776 *F Query: 809 TGCTTGCTTAAACTTGTTCG-----A-AA-TGATGCCAGCGTGTTGAGCCCATGCGTCGA 861 *F | | ||| || ||||| || | || || |||| || |||||||| | | *F Sbjct: 3777 TACCTGCCTACGCTTGTGCGCCGCGAGAAGTGGTGCCGACGAACGGAGCCCATCCACCAG 3836 *F Query: 862 GAAGACCTGGTATACATCTGCGATCCGGCCATACCACGCAGGGAGTGGAA-AAGAGTTGT 920 *F | || |||| | | |||||||||| ||| | | || | ||||| ||| || | *F Sbjct: 3837 GGTGATGTGGTCTTCGTCTGCGATCCTGCCTTGGCCCGACGAGAGTGCCGCAAGGGTA-T 3895 *F Query: 921 CGTTGAAGAGGTGTTCACCGGGAAAGATGGAGT-GCCACGGCGGGCTTCGGTG 972 *F ||| || ||| | | || ||| |||||||| | || | || ||| |||| *F Sbjct: 3896 CGTGGAGGAGATCTACAGCGGAGCTGATGGAGTTGTCA-GACGCGCTAAGGTG 3947 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137483 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG18288 on Tue Jul 31 12:20:29 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Jul 31 20:20:33 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 31 Jul 2001 20:20:33 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 12:20:29 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG18288 on Tue Jul 31 12:20:29 2001 *F Content-Length: 4800 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG18288 *F Release: 1 *F Gene annotation error *F Gene CG18288 has a mistake in the supporting evidence or functional assignment. *F Comments: CG18288 can be deleted from GadFly--it appears to be part of an *F aurora-like element (just 65% *F identity, but over the whole length, so likely to be transposon sequence). *F Query= CG18288|FBgn0033007|CT41493|FBan0018288 GO: located on: 2R *F 41F3-41F3; |cDNA sequence *F (996 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AB022762|aurora-element DMAURA 4263bp Derived from AB0... 971 2.5e-57 2 *F >gb|AB022762|aurora-element DMAURA 4263bp Derived from AB022762 (d1268008) *F (Rel. 59, Last updated, Version 1). *F Length = 4263 *F Plus Strand HSPs: *F Score = 971 (151.7 bits), Expect = 2.5e-57, Sum P(2) = 2.5e-57 *F Identities = 355/538 (65%), Positives = 355/538 (65%), Strand = Plus / Plus *F Query: 455 CTGATGGAGGTGTCTGGGAAAGGATGGTCCAGTGCGTGAAGAAGGTTTTGGCG-CATACG 513 *F |||| || || || ||||| | ||||| |||||||| |||| || || || ||||| *F Sbjct: 3451 CTGAGGGCGGAGTTTGGGAGCGCATGGTGCAGTGCGTCAAGAGAGTACTG-CGTCATACC 3509 *F Query: 514 ATGAAGGAACTTGTACCCAAGAGGCACGTGCAGGAGAACTTGCTGATCGAGGCGGAGAAT 573 *F ||||||| ||| || | | || || ||||| || ||||| |||||||||||| *F Sbjct: 3510 CAGAAGGAAGTTGCGCCGAGGGACCATGTATTGGAGAGTTTCCTGATTGAGGCGGAGAAT 3569 *F Query: 574 ATAGTGAATTCCCGCCCGCTCACGCACTTGCCGATCACAATGGACCAGAAGGCTCCACTG 633 *F || || || || || |||||||| |||||||| | |||||||| |||| || || *F Sbjct: 3570 ATTGTAAACTCGCGTCCGCTCACCCACTTGCCTGTGGATGTGGACCAGGAGGCGCCGTTG 3629 *F Query: 634 ACACCGAACGACCTGTTGAAAGGAATGCCCGACGTGCCAAATATTCCTGGAGATGATGAA 693 *F || || ||||| || | || ||| | || | |||| ||| |||||| |||| *F Sbjct: 3630 ACGCCAAACGATCTTCTCAAGGGAGTAGCCAATCTGCCGGATACGCCTGGATTGGATGCG 3689 *F Query: 694 TTGGCATCTGAGAGATGCACAACAAGGAAACAGTGGCGTATCGCTCGGATGATAAGAGAT 753 *F | | || | | || || ||||| |||||| | || |||| || || |||| *F Sbjct: 3690 GAGCTGCCCAAGGAAGGTACTACGAGGAAGCAGTGGAGAATTTCTCGCCTGCTACGAGAC 3749 *F Query: 754 CGGTTCTGGAAATGGTGGGTGCACG-AGTACTTGCCCACACTTGTTGGCAGAGAGAAAGG 812 *F || ||||||| |||||| || | ||||| |||| || ||||| || | ||||| | *F Sbjct: 3750 CGTTTCTGGAGGAAGTGGGT-CATGGAGTACCTGCCTACGCTTGTGCGCCGCGAGAAGTG 3808 *F Query: 813 GTGCCAGCGTGTTGAGTCCATGCGTCGAGGAGACCTGTTATACATCTGCGATCCGGCCAT 872 *F ||||| || ||| |||| | | || || || | | | |||||||||| ||| | *F Sbjct: 3809 GTGCCGACGAACGGAGCCCATCCACCAGGGTGATGTGGTCTTCGTCTGCGATCCTGCCTT 3868 *F Query: 873 ACCACGCAGGGAGTGGAA-AAGAAGCGTCGTTGAAGAGGTGTTCACCGGAAAAGATGGAG 931 *F | || | ||||| ||| | |||| || ||| | | || |||| ||||||| *F Sbjct: 3869 GGCCCGACGAGAGTGCCGCAAGG-GTATCGTGGAGGAGATCTACAGCGGAGCTGATGGAG 3927 *F Query: 932 T-GCCACGTCCGGTTTCGGTGAGGACTAGCGAT--CGAGCCAAATTAGGGCTATCGAT 986 *F | | || | | | | |||| | | ||| || ||| | |||| | || ||| *F Sbjct: 3928 TTGTCA-GACGCGCTAAGGTGCGCGTGAACGAAAACG-GCCTATCTAGGACAAT-GAT 3982 *F Score = 447 (73.1 bits), Expect = 2.5e-57, Sum P(2) = 2.5e-57 *F Identities = 177/282 (62%), Positives = 177/282 (62%), Strand = Plus / Plus *F Query: 1 ATGTGCCACCCAAACGTGGAGGCCACCATCGGCGAGATTCGTCGAAAGTTTTGGATAACT 60 *F ||| || | ||| ||||| || || |||| ||||| || || ||| ||| | || *F Sbjct: 2929 ATGAAGCATCAAAATGTGGATGCTACGATCGCGGAGATCCGGACAATGTTCTGGGTCACA 2988 *F Query: 61 AACGTGAGGAGGCTGCAACAGAAGGTGGTGGCTAATTGTAACGTATACAAGTTGCAGAAG 120 *F || ||||| | || | || || | | || |||| | |||||||||| *F Sbjct: 2989 AAGATGAGGCGTGTGATGCGGAGAGTCATCTCATCGTGCAACGAGTGCAAGTTGCAGCGA 3048 *F Query: 121 GCACGACCGACACAACCGGAGATGGG-CCCATTGCCGGAGGATCGGCTTGAGACAAATGG 179 *F || || |||| | ||| ||||| ||| | ||||| || || || | ||| *F Sbjct: 3049 GCGCGGCCGATGCCGCCGATAATGGGACCCCAT-CCGGAAGACAAACTGGATGCGGGTGG 3107 *F Query: 180 ATGGCCATTTAAATTCACTGGCCTAGACTATTTCGGACCATTGCTTGTGTCGATTGGGCG 239 *F ||||||||| |||| ||| || || ||||| || || ||| |||| ||| | | || *F Sbjct: 3108 ATGGCCATTCAAATACACAGGACTGGACTACTTTGGGCCACTGCTGGTGACTGTGTCCCG 3167 *F Query: 240 CCGAACAGAGAAGAGGTGGGTGGCGCTACTCACCTGCCTAAC 281 *F | | |||||| ||||| || | | || || | || *F Sbjct: 3168 TCACAAGGAGAAGCT-TGGGTCGCCTTGTTTACGTGTTTGAC 3208 *F Browser: Mozilla/4.76 en (X11; U; Linux 2.2.18 i686) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0137484 *a Misra *b S. *t 2001.7.31 *T personal communication to FlyBase *u FlyBase error report for CG3549 on Tue Jul 31 12:24:42 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 31 Jul 2001 12:24:42 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG3549 on Tue Jul 31 12:24:42 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG3549 *F Release: 1 *F Gene annotation error *F Gene CG3549 has a mistake in the supporting evidence or functional assignment. *F Comments: CG3549 can be deleted from Gadfly--it appears to be part of a *F Waldo-A-like element (just 76% *F identity, but it looks convincing). *F Query= CG3549|FBgn0032954|CT11930|FBan0003549 GO:enzyme (GO:0003824) *F located on: 2L 39E2-39E2; |cDNA sequence *F (964 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 1901 1.6e-107 *F 2 gb|AC005847|Waldo-B WALDOB 5180bp 1015 4.0e-64 *F 3 gb|AJ278684|pilger DME278684 5108bp Derived from AJ278684... 973 2.5e-51 *F 2 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 1901 (291.3 bits), Expect = 1.6e-107, Sum P(2) = 1.6e-107 *F Identities = 527/685 (76%), Positives = 527/685 (76%), Strand = Plus / Plus *F Query: 2 TGTGTGCGCCGACAAGAAGGGAGAACGGAGATCT-GCGAAG-GAGTTGGGAAAG-ACAC- 57 *F || |||| ||||| || | || | | | || ||| | || | |||| *F Sbjct: 1813 TGCGTGC-CCGACCTACAGAGCGACCCTAAAAGAAGCCAAGAGCCACCTTAATGCACACT 1871 *F Query: 58 CAACTTGGGGT-G-ACC-CGCAATCTGCACCTGTTAAGCAGCAGAACGGAGAAAGCTCAG 114 *F || || | || | || | |||| | | | || ||||||| | ||| || | *F Sbjct: 1872 CATATTAGCGTAGTACAGCTCAATGTCAATCA-TTGCGCAGCAGCTCAGAGCCTCCTG-G 1929 *F Query: 115 CTTTTCCGTGCACGTG-GCGGGCTCTACAGTTGCACGCAGATGTGT-ATTTTTACTGCGA 172 *F | | ||| || ||| | || | | || || | | | ||| | *F Sbjct: 1930 CCCAGAC-TGCGGCTGAGCGCAATGTAGACAT-CATGCTCCTAAGCGAACCCTACGTCTC 1987 *F Query: 173 TGA-AGTCGGGACCATACTCCATGATCCTAGACGAGTCAGGCAGAGCTGCTATCAAGTGT 231 *F || || ||| || || ||||||||||| |||||| |||| | ||| |||||||| || *F Sbjct: 1988 TGGTAG-CGG-ACAATCGTCCATGATCCTTGACGAGACAGGTAAAGCAGCTATCAAATGC 2045 *F Query: 232 TGCAGCTTTCTCCAAGTCCAGGAACTGGCTGCATCACCGATGCGGGGAATCGCACATGCA 291 *F ||||||| |||||| ||| ||||||||||||| | ||| |||||||| ||||| |||| *F Sbjct: 2046 TGCAGCTCTCTCCACGTCGAGGAACTGGCTGCTTTACCTATGCGGGGTATCGCTTATGCG 2105 *F Query: 292 AAGATAAAACACGTGCATATGTACAGCTGCTATGCTCCGCCTAGCGACACTCCCGACCAG 351 *F ||| ||||||||||||| ||||||||||||| |||||||| |||||||| ||||| ||| *F Sbjct: 2106 AAGTTAAAACACGTGCACTTGTACAGCTGCTACGCTCCGCCGAGCGACACCCCCGATCAG 2165 *F Query: 352 TACGAGGAGTTCCTGGAGGCGCTTGGGGACCATGCGAGAGGGCGAAGCCCGAAGATCATT 411 *F | ||||||||| ||||||||||| | |||||||||||||||||||||||||||| ||||| *F Sbjct: 2166 TTCGAGGAGTTTCTGGAGGCGCTCGTGGACCATGCGAGAGGGCGAAGCCCGAAGGTCATT 2225 *F Query: 412 GCAGGTGATTTTAATGTCTGGGCAGTGGAATGGGGCAGCAGAGTATCCAACCCCAGAGGC 471 *F || || || ||||||| |||||||||||||||||||||||| ||||||| |||||||| *F Sbjct: 2226 GCCGGCGACTTTAATGCCTGGGCAGTGGAATGGGGCAGCAGGACATCCAACACCAGAGGC 2285 *F Query: 472 CGAGCAGTGATAGACGTCATGGGAATGCTGGACCTAGTACTGCTGAACGACGGTCGAAAG 531 *F ||||| ||||| |||| |||||||||||||||||| |||||||||||||||| || ||| *F Sbjct: 2286 CGAGCTGTGATTGACGCCATGGGAATGCTGGACCTTATACTGCTGAACGACGGACGGAAG 2345 *F Query: 532 CCGACATTTAACAACGATAGGGGTACATCATTTATTGACGTTACCTTTGTCAGTAGAGGG 591 *F ||||| |||||||||||||||||||| || ||||||||||||||||||||||| |||||| *F Sbjct: 2346 CCGACGTTTAACAACGATAGGGGTACGTCCTTTATTGACGTTACCTTTGTCAGCAGAGGG 2405 *F Query: 592 CTTGTTGATAACAACAACTGGATGGTCTATGATTTTGTAACGCTGAGCGACCACGCTTTG 651 *F || || || ||||| |||||||||||| |||| | | ||||||||||||||||| || *F Sbjct: 2406 CTAGTAGACAACAATAACTGGATGGTCCATGACGTCATGACGCTGAGCGACCACGCCCTG 2465 *F Query: 652 ATTTCCTTCATTCCCTCCCCGGAGG 676 *F || ||||||| || ||||||||||| *F Sbjct: 2466 ATCTCCTTCAGTCTCTCCCCGGAGG 2490 *F Score = 626 (100.0 bits), Expect = 1.6e-107, Sum P(2) = 1.6e-107 *F Identities = 142/163 (87%), Positives = 142/163 (87%), Strand = Plus / Plus *F Query: 802 GTGGGAGCAGCTAAAGTCGCTGGCAGGTACCCCGTTCAGCCCTAGTGGCTTGTTCGCGGC 861 *F | | |||||||||||| ||||| ||||| ||||||||||||||||||||||||||||||| *F Sbjct: 4664 GAGAGAGCAGCTAAAGACGCTGTCAGGTTCCCCGTTCAGCCCTAGTGGCTTGTTCGCGGC 4723 *F Query: 862 TATGCTGGCGAACAGCGAGGCATGGGAGCTGGGTCACAGCCTTATAATTAAGATTATGGA 921 *F |||| |||||||||| | ||| ||||||| ||| |||||| |||| ||||| || ||| | *F Sbjct: 4724 TATGATGGCGAACAGGGGGGCTTGGGAGCGGGGACACAGCATTATCATTAATATGATGAA 4783 *F Query: 922 GCGAGTCCGATCAGACGAGATGGCCAACAGAGTTCGTGGCTAA 964 *F ||| ||||||||||||||||||||||||||||| || |||| *F Sbjct: 4784 GCGTGTCCGATCAGACGAGATGGCCAACAGAGTGGATGTCTAA 4826 *F Score = 553 (89.0 bits), Expect = 3.2e-104, Sum P(2) = 3.2e-104 *F Identities = 181/251 (72%), Positives = 181/251 (72%), Strand = Plus / Plus *F Query: 676 GTTTCTCACGGACCATGGCTGCTTTCGGGCCTATTTACTCAGGTTCCGCCACGTAGAGTC 735 *F ||| |||||||||||||||||||| ||||||||| ||||| ||||||| ||||||||||| *F Sbjct: 4526 GTTCCTCACGGACCATGGCTGCTTCCGGGCCTATCTACTCCGGTTCCGTCACGTAGAGTC 4585 *F Query: 736 AGCCCAATGCGTGTTCTGCGTCGATCGTGAAGAAACAGCTGAACATGTGATAATGCACTG 795 *F |||||||||| ||||||||||||| ||||||||||||| ||||||||| |||||||||| *F Sbjct: 4586 AGCCCAATGCTTGTTCTGCGTCGACGGTGAAGAAACAGCAGAACATGTGCTAATGCACTG 4645 *F Query: 796 CTCCAGGTG---GGAGCAGCTAAAGTCGCTGGC-AGG-TACCCCGTTCAGCCCTAGTGGC 850 *F |||||||| || | || | || ||| | | | | |||| || | | | *F Sbjct: 4646 CTCCAGGTTCACGGCGGAGAGAGAGCAGCTAAAGACGCTGTCAGGTTCC-CCGTTCAGCC 4704 *F Query: 851 TTGTTCGCGGCTATGCTGGCGAACA--GCGAGGCATGGGAGCT-GGGT-CACAGCCTTAT 906 *F | | || | || ||| | | |||| || ||| ||| ||| | | | | *F Sbjct: 4705 CTAGTGGCTTGTTCGC-GGCTATGATGGCGAA-CAGGGGGGCTTGGGAGCGGGGACACAG 4762 *F Query: 907 AATTAAGATTA 917 *F |||| |||| *F Sbjct: 4763 CATTATCATTA 4773 # *U FBrf0137485 *a Ayme-Southgate *b A. *t 2001.7.16 *T personal communication to FlyBase *u FlyBase error report for CG10285 on Mon Jul 16 13:57:10 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 16 Jul 2001 13:57:11 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: southgatea@COFC.EDU *F Subject: FlyBase error report for CG10285 on Mon Jul 16 13:57:10 2001 *F Error report from Agnes Ayme-Southgate (southgatea@cofc.edu) *F Gene or accession: CG10285 *F Release: 1 *F Gene annotation error *F Gene CG10285 has incorrect exon/intron structure or translation start site. *F Comments: The gene CG10285 actually represents the NH2-terminus of gene CG1479 *F (also known as bt or projectin). The sequence originally considered intergenic *F between CG10285 and CG1479 is also part of CG1479 (projectin). The corrected *F full sequence is available through the corrected CG1479 annotation file. # *U FBrf0137486 *a Radford *b J. *t 2001.7.27 *T personal communication to FlyBase *u *F From: 'Jonathan Radford' *F To: bdgp@fruitfly.org *F Date: Fri, 27 Jul 2001 14:32:29 \+0000 *F Subject: BDGP gene annotation *F Dear Sir/Madam, *F I have recently been looking at predicted genes for g-protein coupled *F receptors on the sequence database and believe I have found a mistake in the *F annotation of one gene. I thought I should bring this to your attention, *F although it is a bit complicated to explain through the 'fix annotation' *F option. *F I believe that the DNA sequence for the protein receptor NPFR76F has *F actually been wrongly divided as two CG's, CG7395 and CG18729 (CG18639). It *F also requires a small portion of genomic sequence to be included between the *F two CG's as well as full read through to the stop codon contained at the end *F of CG18729 . *F A full length ORF can be gained by reading from the start codon in CG7395 *F (nt 702 of cDNA) through the genomic sequence (AE003514 250814-250921) and *F into the coding sequence of CG18729 to the termination codon. This ORF *F sequence translated gives a matching amino acid sequence to NPFR76F. *F Yours faithfully, *F


Jon Radford *F Dow/Davies Lab *F Molecular Genetics *F Anderson College *F IBLS, Univ of Glasgow *F Tel: 0141 3306811 *F radford_jon@hotmail.com # *U FBrf0137487 *a Misra *b S. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG2528 on Mon Jul 30 15:24:39 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 15:24:39 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG2528 on Mon Jul 30 15:24:39 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG2528 *F Release: 1 *F Gene annotation error *F Gene CG2528 has incorrect exon/intron structure or translation start site. *F Comments: CG2528 matches part of roo's terminal repeats from 2141-2404 of its *F 2404 *F nucleotides. *F Query= CG2528|FBgn0032969|CT7910|FBan0002528 GO: located on: 2L 40A6-40A6; *F |cDNA sequence *F (2404 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F FB|FBgn0000155|roo DM_ROO 9092bp 1258 4.4e-51 1 *F >FB|FBgn0000155|roo DM_ROO 9092bp *F Length = 9092 *F Plus Strand HSPs: *F Score = 1258 (194.8 bits), Expect = 4.4e-51, P = 4.4e-51 *F Identities = 260/266 (97%), Positives = 260/266 (97%), Strand = Plus / Plus *F Query: 2141 TTGCTG-CCCTTCAGGAAGC-TGTTCACACATGAACACGAATATATTTAAAGACTTACAA 2198 *F |||||| ||||| || || ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 8645 TTGCTGGCCCTTT-GGCAGAATGTTCACACATGAACACGAATATATTTAAAGACTTACAA 8703 *F Query: 2199 TTTTGGGCTCCGTTCATATCTTATGTAAATGAATCGAGAGCGATAAATTATATTTAGGAT 2258 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 8704 TTTTGGGCTCCGTTCATATCTTATGTAAATGAATCGAGAGCGATAAATTATATTTAGGAT 8763 *F Query: 2259 TTTGTTATCTAAGGCGACATGGGTGCATTGCTCAAAAACATGTAATTTAAGTGCACACTA 2318 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 8764 TTTGTTATCTAAGGCGACATGGGTGCATTGCTCAAAAACATGTAATTTAAGTGCACACTA 8823 *F Query: 2319 CATGAGTCAGTCACTTGAGATCGTTCCCCGCCTCCTAAAATAGTCCCTTAGTGGGAGACC 2378 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 8824 CATGAGTCAGTCACTTGAGATCGTTCCCCGCCTCCTAAAATAGTCCCTTAGTGGGAGACC 8883 *F Query: 2379 ACAGATAAGGTCCTCGCCGCTCAAGA 2404 *F |||||||||||||||||||||||||| *F Sbjct: 8884 ACAGATAAGGTCCTCGCCGCTCAAGA 8909 *F Score = 1225 (189.8 bits), Expect = 1.7e-49, P = 1.7e-49 *F Identities = 245/245 (100%), Positives = 245/245 (100%), Strand = Plus / Plus *F Query: 2160 TGTTCACACATGAACACGAATATATTTAAAGACTTACAATTTTGGGCTCCGTTCATATCT 2219 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 TGTTCACACATGAACACGAATATATTTAAAGACTTACAATTTTGGGCTCCGTTCATATCT 60 *F Query: 2220 TATGTAAATGAATCGAGAGCGATAAATTATATTTAGGATTTTGTTATCTAAGGCGACATG 2279 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 TATGTAAATGAATCGAGAGCGATAAATTATATTTAGGATTTTGTTATCTAAGGCGACATG 120 *F Query: 2280 GGTGCATTGCTCAAAAACATGTAATTTAAGTGCACACTACATGAGTCAGTCACTTGAGAT 2339 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 GGTGCATTGCTCAAAAACATGTAATTTAAGTGCACACTACATGAGTCAGTCACTTGAGAT 180 *F Query: 2340 CGTTCCCCGCCTCCTAAAATAGTCCCTTAGTGGGAGACCACAGATAAGGTCCTCGCCGCT 2399 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 CGTTCCCCGCCTCCTAAAATAGTCCCTTAGTGGGAGACCACAGATAAGGTCCTCGCCGCT 240 *F Query: 2400 CAAGA 2404 *F ||||| *F Sbjct: 241 CAAGA 245 # *U FBrf0137488 *a Misra *b S. *t 2001.7.30 *T personal communication to FlyBase *u FlyBase error report for CG10408 on Mon Jul 30 15:27:40 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 30 Jul 2001 15:27:40 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG10408 on Mon Jul 30 15:27:40 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG10408 *F Release: 1 *F Gene annotation error *F Gene CG10408 has incorrect exon/intron structure or translation start site. *F Comments: CG10408 matches part of the Waldo-A transposon from 1392-2328 of its *F 2328bp. *F Query= CG10408|FBgn0033023|CT9331|FBan0010408 GO:[multicopper ferroxidase *F iron transport mediator (GO:0004323)] located on: 2R 41F6-41F6; |cDNA *F sequence *F (2328 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 1678 2.4e-130 *F 6 gb|AC005847|Waldo-B WALDOB 5180bp 262 4.3e-05 *F 1 gb|AJ278684|pilger DME278684 5108bp Derived from AJ278684... 247 0.00021 *F 1 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 1678 (257.8 bits), Expect = 2.4e-130, Sum P(6) = 2.4e-130 *F Identities = 422/515 (81%), Positives = 422/515 (81%), Strand = Plus / Plus *F Query: 1805 TAGACGAGTCAGACAAGCGTATCAAACCCAATAAAGCCGCTGGACTAAATGGAATTCCTG 1864 *F | | |||| ||| |||||| ||||||||||| |||||| |||||||| |||| ||||||| *F Sbjct: 3076 TCGCCGAGGCAGCCAAGCGCATCAAACCCAACAAAGCCCCTGGACTAGATGGTATTCCTG 3135 *F Query: 1865 GTGCAGTTGTTAAAGCATTGGCGTTCGGTAGACCTGACATCTTCAGGGCCACCTTACAGC 1924 *F | || ||| | |||||| ||||| | ||||||||||| ||||||||||||||||| |||| *F Sbjct: 3136 GAGCTGTTATAAAAGCAGTGGCGCTGGGTAGACCTGAAATCTTCAGGGCCACCTTCCAGC 3195 *F Query: 1925 AATGTCTTTTGGACGGAATCTTTTCAATAAGGTGGAAAAGCCAGAAAATAGTTCTGCTGC 1984 *F |||| ||| ||||||||||||| ||| |||||||||||||||||| |||| ||| ||| *F Sbjct: 3196 AATGCCTTCTGGACGGAATCTTCCCAACAAGGTGGAAAAGCCAGAAGCTAGTCCTGTTGC 3255 *F Query: 1985 ACAAATGCAAGGGATCAGCATATGCTGCAAACAGCTACCGCCCTCTATGCCTACTAAATA 2044 *F ||| |||||||| ||||| |||||||||||||||||||||||||||||||||| ||| *F Sbjct: 3256 CGAAAGGCAAGGGACCAGCACATGCTGCAAACAGCTACCGCCCTCTATGCCTACTGGATA 3315 *F Query: 2045 TAGTTGGAAAACTGTCCGAAGATATCTTGTATACTCGAATAGAGGCCGTC---GAT-GCT 2100 *F |||| |||||||||| |||| |||| ||||||| |||||||||| || || || *F Sbjct: 3316 TAGTAGGAAAACTGTTCGAACGTATCCTGTATACCAGAATAGAGGCAATCACCGAGAGCA 3375 *F Query: 2101 CTCAGTGACGAAGGAAGTCAGCAATATGGCTTTCGGAAGGGAAAGAGTATCCTGGACACT 2160 *F ||| | | |||||||| ||||||||||| ||||| || ||||| | |||||| || *F Sbjct: 3376 \-TCAACGGCCTGGGAAGTCATCAATATGGCTTCCGGAAAGGTAAGAGCACTCTGGACGCT 3434 *F Query: 2161 CCTTTGACCGTGAGTAACATCGACAAGACCGCTCTTGCTGGTGATACATGGCTAGTTGGC 2220 *F | || | |||| ||||||||| |||||||||| || ||||||||| |||| ||| ||| *F Sbjct: 3435 CTTTCGGCCGTTTGTAACATCGCCAAGACCGCTATTTCTGGTGATAGATGGTTAGGGGGC 3494 *F Query: 2221 AGGGAGGAATACTACGCAATAGTGACTCTGGACGTAAAGAACGCCTTCAACACCGCCAGA 2280 *F ||| ||||||||| |||||| |||||||||||||||| |||||| ||||||||||||||| *F Sbjct: 3495 AGGAAGGAATACTGCGCAATTGTGACTCTGGACGTAAGGAACGCTTTCAACACCGCCAGA 3554 *F Query: 2281 TGGGCCGCAA--CTCAGCAACTTTGGGTACCGTGT 2313 *F ||| ||| || ||| || | || |||||| | *F Sbjct: 3555 TGGCCCGTAATCCTC-GCGGCCATG--TACCGTAT 3586 *F Score = 583 (93.5 bits), Expect = 2.4e-130, Sum P(6) = 2.4e-130 *F Identities = 143/176 (81%), Positives = 143/176 (81%), Strand = Plus / Plus *F Query: 1523 AAGTGCCCAAGGAGCAAGTTCCCAACGTTCAGGCGCCCAAGCCGAACTACCAAGCGACTG 1582 *F | || ||||||| |||||| || || | |||||||||||||||| | ||| || | *F Sbjct: 960 AGGTACCCAAGGTGCAAGTGCCAAAAATCCAGGCGCCCAAGCCGAGCAACCCTACGTTGG 1019 *F Query: 1583 GTAGCAAGGGAGAAGGAAAGGCGACATCCAGAGCAAAAGAGTCCAAGCCAGGCAGCTATG 1642 *F || |||| || |||| |||||| ||||||||| |||| |||||||| ||||||||||| | *F Sbjct: 1020 GTGGCAACGGTGAAGAAAAGGCAACATCCAGACCAAACGAGTCCAAACCAGGCAGCTACG 1079 *F Query: 1643 AATCTGTCTCCCAGAATTCTAGCACGACTGCAGAGTGGACCAAGGTGAAGCCTAAG 1698 *F ||||||| || ||||| |||||||| || ||||||||||||||||||||||| | *F Sbjct: 1080 CATCTGTCGCCAAGAATGCTAGCACGGGTGTAGAGTGGACCAAGGTGAAGCCTACG 1135 *F Score = 370 (61.6 bits), Expect = 2.4e-130, Sum P(6) = 2.4e-130 *F Identities = 82/92 (89%), Positives = 82/92 (89%), Strand = Plus / Plus *F Query: 1392 AGGTGGAGAACTTGGCTCCTACAGCATCTACTATCGCGACAAGCCAGAAGACGATGGACT 1451 *F ||||||||||||||||||||||||||||||| | ||||||||| ||||||||||||||| *F Sbjct: 576 AGGTGGAGAACTTGGCTCCTACAGCATCTACGACCGCGACAAGTCAGAAGACGATGGACC 635 *F Query: 1452 ACACTACCATGCCATCGCAAAAGGCGGGATGG 1483 *F ||| || |||||||||||||| ||||| || *F Sbjct: 636 ACATCGCCGTGCCATCGCAAAAGACGGGAGGG 667 *F Score = 331 (55.7 bits), Expect = 2.4e-130, Sum P(6) = 2.4e-130 *F Identities = 113/155 (72%), Positives = 113/155 (72%), Strand = Plus / Plus *F Query: 1683 CAAGGTGAAGCCTAAGAAGAGGAGCTCCACAGCTGCATGGTCGCTCAATCGCGGTGGAAC 1742 *F ||| || || | | | || ||||| | ||| | ||| | | | | |||| || | *F Sbjct: 1892 CAATGTCAATCATT-GCGCAGCAGCTC-AGAGCCTCCTGGCC-CAGACT-GCGGCTGAGC 1947 *F Query: 1743 GCGATGTAAACATCATGCTCCTAAGCGAACCTTACGTCACTGG--G-G-ACAATACTCCA 1798 *F || ||||| |||||||||||||||||||||| |||||| |||| | | ||||| |||| *F Sbjct: 1948 GCAATGTAGACATCATGCTCCTAAGCGAACCCTACGTCTCTGGTAGCGGACAATCGTCCA 2007 *F Query: 1799 AGATCCTAGACGAGTCAGACAA-GC-G-TATCAAA 1830 *F |||||| |||||| ||| || || | ||||||| *F Sbjct: 2008 TGATCCTTGACGAGACAGGTAAAGCAGCTATCAAA 2042 *F Score = 196 (35.5 bits), Expect = 1.9e-100, Sum P(5) = 1.9e-100 *F Identities = 96/149 (64%), Positives = 96/149 (64%), Strand = Plus / Plus *F Query: 1660 TCTAGCACGACTGCAGAGTGGACCAAGGTGAAGCCTAAGAAGAGGAGCTCCACAGCTGCA 1719 *F |||| | | | || || ||||| | | | | | | || ||||||||||||||||||| *F Sbjct: 1396 TCTATCTTG-CAGCGGATTGGACGAGGCT-ACGAC--AGCAGAGGAGCTCCACAGCTGCT 1451 *F Query: 1720 TGGTCGCTCAATCGCGGTGGAACGCGATGTAAA-CATCATGCTCCTAAGCGAACCTTACG 1778 *F ||||||| |||| | | || || | |||| | | | | | | || | |||| || *F Sbjct: 1452 TGGTCGCCCAATTCCAG-GGCCTGC-AGATAAATCCTGAAGATA-TCAGGGG-CCTT-CG 1506 *F Query: 1779 TC-ACTG-GGGACAATACTCCAAGATCCT 1805 *F | || |||| || | || | ||| *F Sbjct: 1507 CAGAATGCGGGATGGCACGCAAATAGCCT 1535 *F Score = 172 (31.9 bits), Expect = 2.4e-130, Sum P(6) = 2.4e-130 *F Identities = 40/47 (85%), Positives = 40/47 (85%), Strand = Plus / Plus *F Query: 1482 GGTCTTCGGACCTGACCACGGCTCTCCAGAGCCAGAACCTGAAGTGC 1528 *F ||||||||||||||||| |||||||||||| || |||||| | || *F Sbjct: 693 GGTCTTCGGACCTGACCGCGGCTCTCCAGAATGAGGACCTGAGGGGC 739 *F Score = 130 (25.6 bits), Expect = 2.4e-130, Sum P(6) = 2.4e-130 *F Identities = 30/35 (85%), Positives = 30/35 (85%), Strand = Plus / Plus *F Query: 2293 CAGCAACTTTGGGTACCGTGTCCCATGGTACGATA 2327 *F |||| ||||| || |||| |||| ||||||||||| *F Sbjct: 3620 CAGCTACTTTAGGGACCGGGTCCTATGGTACGATA 3654 # *U FBrf0137489 *a Mount *b S. *t 2001.8.14 *T personal communication to FlyBase *u *F In the list below the introns are suspected of being an *F annotation error because the intron is extremely short, because the 5' *F splice site does not conform to consensus, or both. In some cases, the *F intron is displaced relative to its actual position. In others, there *F may be no intron at all. In all cases, the evidence for the intron *F should be reviewed. *F The list contains lines like: *F 17860935 17860909 2R CG11716:2 26 *F GATGAGAACGAAAAAAAATGAAAAAAAAACGAAAAATCAAT *F The penultimate field is the length of the intron, and the sequence is the *F intron itself plus 10 nt. upstream and 5 nt. downstream. *F Steve Mount *F \------------------------------------------------------------------------------ *F -- *F 17860935 17860909 2R CG11716:2 26 GATGAGAACGAAAAAAAATGAAAAAAAAACGAAAAATCAAT *F 2828076 2828061 3R CG1028:1 15 AAGAACATCTAAAAAAGATAAAAAATCGAG *F 4217476 4217435 2R CG8024:5 41 *F CAAGACCAAGAAAAAGAGCCTGACGCGCCGCCTGCTTCTTGGAGGCCTGAGAAAAA *F 4750702 4750767 3R CG11963:9 65 *F GATTGCACAAAAAAAGAGGAAAAGAAAAAGGAAGAAAAGAAAGACATTTGCGAAAAGAAAGGTAAAAAGTAAAGGGATAC *F 8747854 8747891 3L CG4974:1 37 *F GGAAAAACTGAAAATATCGGTGCGAAATTCGAGCGGAAAAACGCTAGAAAAG *F 11486731 11486686 X CG2448:5 45 *F AGTTGAAAGAAAACAAAAACAAAGATAAAAAACGAACGCAAAACAAAACTAAAACTGATA *F 6576625 6576694 U CG12460:3 69 *F GCCCACCTTTAAACCACCTTTAAACTATCATCCTAATGCCGAGAAGGCTAAGCGCGCCCTGGACGGATCGATGCGAATG *F GTGTA *F 7421538 7421612 3L CG16992:2 74 *F ATGCTGGATAAACAGTGGGTTTTAGGGTAGAGTGTTTAAGCCAGTTTAAGACCAGTAATTGATTTTCTTTGAACAGCCT *F ATGACTGGTC *F 14024831 14024911 X CG1517:14 80 *F TAGGGCCCAAAACGCCAGCGTAAGTAGCGATGGGTCTTCATTGTGAATATAATATAAGAATTACATTTAAATTTGTTAT *F GTACCCTTCAGAAGCA *F 9846925 9846964 2L CG4619:1 39 *F AGAGAGTTGAAACTATATAAACACAGTTTAACACATCGTTTTGTTTCAGCTAAA *F 14960345 14960361 2L CG15270:1 16 GAATGAGCCAAACTTTTAAAACTTTAATGCA *F 11605947 11606010 3R CG4546:1 63 *F TGACTTCGGTAAGTGATATATAAGAAGCATAATAGATATGGATTCTTATGTAGCCACTTTACCTGCTTCTCCAGATCG *F 8194909 8194832 3R CG17227:1 77 *F AGAGCAGGGCAAGTTGTCATTTTTCAAGAAACTTCCGACACAAACTCTTGTTGAGTCTGGTCTAACTTCTCGTTTCCCG *F TAGATGGCGACGT *F 2830285 2830230 2R CG12055:1 55 *F TCCCCTGGCCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGTCAT *F 1579159 1579121 3L CG12104:2 38 *F ATATTTACCAAATAAGTAAAATTTATAAATAATTTTAAAAATAAAATGTTAGA *F 4509091 4509129 X CG6998:3 38 *F ACACACACACACACACACACACACACACACACAAACACACACACACACGGACA *F 11805824 11805779 3R CG4201:4 45 *F GTAAGTTAATACAGAAAACTGTTAAAAATTTACTAATAAATCATTTGGGTTACAGACTTG *F 6003419 6003364 X CG3648:5 55 *F GCCTTTCCTCACAGGTTAATAATAATAGTAATATATAATTTAATAGAATTATATCAATCGCTTTCTTGGC *F 5172226 5172303 3R CG18473:6 77 *F TGACCCCTAAACCTAAAAAGTTTGTTATTCTGGTAGCATCTCTTATCATGTTTTTCTTGTTGATAAGATTAGCGCGGTT *F CTTTTGGCAATAA *F 9164046 9164009 2L CG17011:1 37 *F TCCACAGTCAACCTCACCCCGCAATCCCGGCCAGATTTCCCCTGACAATGAC *F 7854710 7854644 3R CG14740:4 66 *F AAAGTGCTAGAGAACTAAGGTGAATGTTTGCTTTCATTATTCTAAACTTTCAAACAAATATTTTCTCTCTTAATAGATA *F AC *F 22678468 22678537 3R CG18472:6 69 *F GGAAATTTAGAGAACTGCATACAGTGTCTGCAGCGTGATGTTTTTACGATTGTTTAAAAATATATACATATGTAATGCA *F AATAA *F 528668 528712 X CG5273:3 44 *F TGGCTTTTAGAGACCATGCTTCTGGAACGTTCCACTGTGCACCCACTCAATTACCCGAC *F 3196268 3196204 2R CG8708:2 64 *F CTTTACTACAAGACGGATGACGTAAGTTTCGCCTGATAAGGGATAGGTATTCTTAACATCTTGTGTTCCGACAGGGTCT *F 12768031 12768085 3R CG8907:2 54 *F CTTCCAGGTGAGCAGCCACGATTTTTCAGCACTTTGAGATCTGTAATAATTAGATCATTAATAGGTGGC *F 9397087 9397009 X CG3004:3 78 *F ACTGAACTGAAGCATCAACTAAAACAACATCCACACAACAATTGAACAGATTAATGAGAATACCTCTGAAAATGAGCGC *F TTGGCTTTTGTAGC *F 9815336 9815258 3L CG6707:4 78 *F TGCTTCGGTAAGCCAAATCAATTTAAAATCAAGCTGAAGTATGTGATTTTCTAAACCCATATGTGTGTTTCCTTTTGCA *F TTTTTCCAGGATCA *F 15771723 15771655 2R CG17999:2 68 *F GCATTACAGCAGCCGCGTAAGTTTCTGTGTATTTCGCGATGAACTAAATCAATTCTAATAGATGTTTTCTAATTTTAGG *F TATG *F 15973563 15973607 2L CG4892:1 44 *F AAAACGCTGGAGGAATCTGCAAAGTTGGCCACGGATGCCGATTCCGTAAAGAGTATGGA *F 7689844 7689784 3R CG6977:8 60 *F AACCGCGCCAAGTAAGTGTACTTTACTTTTGCGAAGAGCTTTTCTAATGTGTAATTCTCAAATCCGTTAGCTACG *F 20060506 20060558 3R CG18670:4 52 *F CAATCAACAAAGTAAGTTCGAATTTGTTTCAATAAGCGGTAGCTAACTGTTTTTTCCTGCAGAAAAT *F 1663690 1663741 3R CG1208:4 51 *F TTAATTGGTAAGTGATGTAAACTTGCCAGTTTGAATTCGATTTGAATGTGCAGCACTTCAGGTATC *F 2375669 2375613 3R CG10690:2 56 *F TCGTGCTGTTAGTTAGAGTACGGATGAAGTGAGAGATAGCCAAGTGCGCGACAGGTGTCCACTCACCTTGA *F 646780 646841 3R CG1277:1 61 *F GGCAATGTCAAGTTGCTAAGCGAAGAAAGCAGTCATTAGACATAAATTTGAATGTTAAACTGGACACTTACCTGGG *F 9449390 9449448 3R CG9351:11 58 *F ACAAACCAAAATAAATTGTATTTAGTAGTTAAATCCCCCTCTCTCCAAACTGATATGAACAGCAGCAGCAGCG *F 7939447 7939495 2L CG7392:5 48 *F TATGCATACAATAATAAGAAAGGGAAAAGAGAACACAACATAAACATACAAATAATTGCTAAA *F 16246488 16246519 2R CG9696:1 31 CGTCGAACTGATACCAACCTTCTGTCTGATACGGCGAACAGCAGTA *F 20361942 20361957 3L CG5100:5 15 GATTTAGGCAATATATATATATATAATGCG *F 1093302 1093356 4 CG11148:6 54 *F TTGATGGTAAATATTTGCATAATTGTTATATATTTAACTGTATTCATTTTCATTACATTTTTAGTACCC *F 23662918 23662846 3R CG4976:4 72 *F AAACTCAAGGATGATGCAGTTAAGGTAAGCTGTTCAAGAAATACCCTATTTATGGAGTTATAAACCTATTATTCTTTTA *F AAGGCCCA *F 6550270 6550209 X CG3203:4 61 *F TCTTGACCGGATGTGTTATTTACACATATCAAATATTAAGATATCTTACTAATTGCCCATGTTTCTTGCAGATATT *F 17593271 17593231 3R CG6575:2 40 *F ATGATTAATAATTAATAATAGTAATATATCCGGATGCATTGCGATATCGGTCTTA *F 11811999 11811944 X CG11366:1 55 *F TGTAAATATTATTAGACACTTTTTCTTACATTGCAACATCACTTAAAAAACAAAAAAAAAGTTACCGATT *F 11619163 11619096 3R CG4224:6 67 *F TGATACGCAGATTTAATAAGTAAGTACTAACTGAATCCATAGTTATGGATTCCTCACTAACTTTGACTGCTTTACAGAG *F ACA *F 17020448 17020436 3R CG6056:1 12 GGTTTAACCAATTTCCCGAAAAATGGT *F 7583359 7583321 3R CG6939:9 38 *F GCCACGCCCGCAAATTTTTTGATATAATTTTTTATATATATACATATATCCTT *F 20042622 20042577 3L CG13812:2 45 *F ACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAATAATAGCAGTA *F 8929877 8929826 3L CG5194:1 51 *F AAAAAAATGGCAAGTAGAATAGATTTTTATGTGGCCCATAGTGTAAGATAATTTCCCGTAGGTTGG *F 16013610 16013538 X CG9910:5 72 *F TAAGTGAAAGCACTTACTCATTAAGCGAAGCACCCTTCCAAATCTCGTCATCCCCGTTATCTTTTTTGACAAAAATAAT *F CAGTATTT *F 18909539 18909476 2L CG10699:7 63 *F GCAGCAGCAGCAGCATCAGCCACATTCGTCGCAGCTATAGCAGCGGCATCACACTGAACGCTCGCAACCGCAGCAGCA *F 7795994 7796027 X CG1543:1 33 TCATCACAAGCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA *F 19237792 19237727 2L CG18576:5 65 *F CCGGATGGACCAGGTATGTAAATACCAGCTAACTCAATTATTTGATTGTGAACTAAAATGTTTGTCCCATTTCAGTTGGC *F 15564187 15564217 X CG8544:12 30 ACATCGGCAACATCAGCAACATCAGCAACATCGGCAGCAGCAATA *F 15893555 15893495 3L CG5830:5 60 *F AAAGACGTAACATCAGTGGGAACTAATGTTTATAAATATATGATAAATTGTTTACATATTACAAGACCAAGCAAG *F 9205307 9205382 3L CG4481:15 75 *F TGGGCTTGGGCATGGGACTGGGCGGCGGCATGAGTGGCGGTAGTCTGGGTAAGGCCAATGGATCCATGATGTTGGGTCC *F ATCGAGCGCCG *F 4495722 4495690 X CG2984:1 32 CGGGGGAAAACCACAAAAAATAACAGAGGGAGAAGCAGGAAGATGTT *F 1082166 1082098 3R CG12173:2 68 *F CACGGATATTCCAGGAGGTGAGTGGCTGATCCACACGAATCCTCATCCGTAACATTTTTAATGTGAAATTATATTTTAG *F AAGC *F 17868183 17868121 X CG5659:8 62 *F AGCTACGCCCCCATTCTTAGTTCATATGTAATGGCCTCTCGAATGTTGCAAATCCTTAATGGAGATCAGTGCTCCCA *F 11945108 11945069 X CG1924:1 39 *F CTGCCAGATGCCGACATGATTTTTGATCCAACTGCCACGAAACCTGAAGATTGG *F 3943325 3943265 2R CG8258:1 60 *F GCGCCAGTCACCGTTTACGATAATCGATAGGCAAGTGCCATCGCTATCCCATCCAGAATGTTCGCGGCCACTTTG *F 8380592 8380667 2R CG4740:2 75 *F GCGGCGCCACCCTGGGCTACAACAAGTGAGTCTCTTAAAGGATTACGATCTGAGCAATAGCGAAACTAATCTTATTTTT *F CATGAGCAGTC *F 25958904 25958925 3R CG9682:5 21 CTGGAAATGTCGCTGGAAGTGCAGCAAATGCTGCTG *F 13489863 13489819 X CG10986:10 44 *F GACACGACGACGGAGGGCGGCATTCCGCCGCTGGCCATTGAGATTGTCCAGGAGATGAC *F 19602373 19602322 X CG15618:4 51 *F GCTCGTCCTGCGGCCCGTCGATTATACAGAAAACGCTACACTAGCACTGGCTATTCTGAAGAGGAT *F 13557244 13557296 2L CG18507:3 52 *F GACGGTAAGTCGGGCAAACTGTTCTAACTGGAAAATAGTTAAATAAAATTATACTTTTCCAGCTCAC *F 20685914 20685859 2L CG9337:4 55 *F GTACAGCAAACGTAAGTGTTTGGAGTCCATATCCGAATCTTACTGACATGAAATTTATGCGCAAGCCGCC *F 21276976 21276925 3L CG5664:3 51 *F GCAGTTGTCGCGTGAGGATTACCTAGCATTACTCCTTAGCGCCTTAAAAGACTATGACAAGTGGTT *F 16502581 16502641 3L CG18860:2 60 *F CACCAGGCAGCGTGAGTTAACTGGTTACCTTTTAATGACTATGATACAGTAACGGATGCATGTTTTTTAGAATCA *F 12412254 12412202 2R CG6530:2 52 *F CACTCCAGAACGTGGAAGACCGTTGGTAAGCTGAATTCCCTGCTTGACTTTAAGGTTTTTAGTTAAT *F 11855121 11855063 X CG11359:1 58 *F TTGTCAGGTGCTAAGGGAAAAATTTGTTTAGTTAATGGAAAAATATAAGGCACAATTTATTTACTTACTTTTT *F 19731355 19731314 3R CG5405:11 41 *F CGTCTATGTACTACCTAAATCAGAAAAATATCTGTGTGTGAAATTATATACAAAAC *F 11854974 11854900 X CG11359:2 74 *F GGGTGAAACTCTAGAAATACAAATGTTGTACGATTTCTGTGTAGTTTTTCCATATGTTTGTGGTTTGTTAAATTTGTAG *F CTTACCAATT *F 2864572 2864510 2R CG18495:4 62 *F AAGTGAGAGACTAGGAAAAAATTATAAAATCATTTTAGTCTCAAATCAAAAGAAAAAGAATCCGTGGTGAACCTTTT *F 16094621 16094684 2L CG4935:4 63 *F TGATTATTAGCTATATACATATATTAACATGTGGGTTTCCTTTGTCATATATCCGTCAGGTGATAATGGCGGCGTCGT *F 4254271 4254214 3L CG11583:2 57 *F CCACTACCATCTATTAACGAGTCATAGGTTAGCCATAAGCTTGGTCGTATTGTAAAGGAATACCTACCTCAA *F 11156705 11156639 2L CG6392:10 66 *F ATGCCCATGCCTATTGTCGAGGGTGAGGAGACGAATTACTTACTGGAAAAACTTAATATGCAAAGGTAAAACTCACCGA *F AT *F 21092849 21092818 2L CG8672:1 31 TCGACAATGCCTCCCATGAGGGAACGCATCCCTCAGATCAGATGGA *F 15249872 15249814 2R CG13867:1 58 *F AGTTGTATGCCTGAAAGAAGATCGTTTTTAGTTCTAACGCCAAACTGCTGAAGTTCTCTTGAACTTGCCATAA *F 16363264 16363320 3R CG5206:11 56 *F GGCGTAGTAACTGAAATGGATTATTTTTTAATTATTTTGTTAATCAGTTTTGAATCATTATCCTACTATGT *F 2468882 2468827 3R CG1315:3 55 *F CGAAGAAAAACTGAACGAAAAAGATAGAATTAATTAAAATGTCATACTTTTGTTTTTGTGTTTACCTTAA *F 21092318 21092288 2L CG8672:2 30 TCACGTGCTTCTGCATTGGTAATTGCTGGTAAGTCGTGGGGAACC *F 20219992 20219923 3L CG5517:14 69 *F AGGGACTTGCCTGTAATTGGATTACCAAGAAATCAGATATAACTAATACGCGGTAGATCCACACCAAATACTGATGTAC *F CTTTA *F 19308840 19308895 3L CG12518:2 55 *F CAAGGACGTCCTGTGAATAAAATACAATTAGTTTATAGGGCAGCAAGCTAAGATCTCAAACTTACGGATT *F 19495556 19495606 X CG12703:5 50 *F TCGCCGGCGTCTTCCTGACACGCCTGCGCCGCCCCAACTGGTCGCCTGACCGTCGAGGAGCAGAA *F 15557856 15557784 3L CG7594:1 72 *F CAAGCGATAGCTTGTTCGGGTAAACCAATATCTTTTCATTAACTTTAAACACTTTCATCAATGTTTAATTGGTTATTTT *F CAGCTCCG *F 6989177 6989108 U CG17419:4 69 *F TCATTGGTAAGAAAGTTATTATCGCGAAATGTAAAATATTATTTCTTTTACATATATACTTTAAATATTTATTTTTTAG *F CCGGC *F 18749756 18749804 X CG12527:2 48 *F TCAGCGATCAGAAGATTCGCAAGGTGCTGTCCGACAACCGGATCGCTAGTGGCTCCAGTGTGG *F 10647739 10647697 X CG1655:1 42 *F AGCAGTTCGAGAAGGAGCTGGACAACCTTAAGGAGTTCTACAACAGCAACAGGAAGA *F 7179690 7179729 3L CG14827:1 39 *F TCCGCCCGCTGAGGATTCCAGCTGTGCCACTCGAAGACGATCCTTATCTGCTCC *F 13973792 13973869 3R CG7985:2 77 *F GCAACAATGAGAGGCGCCAGGTGAGTGCCCTGTTGGCAGTTACCTTAAGTATACCTGAAATATAATGCCATACCATTTA *F ACTTTTAGCCAAA *F 17101709 17101779 3R CG7836:4 70 *F CACGAAGGGTGAGTCCGAATCCTGGCTAGAAGCCAAAATCCTGATCCATGATAACCAAGCTAATTCACGCTCCATTCGC *F AGGCGG *F 1467942 1467872 3R CG2244:9 70 *F ACAAGAATGTGAGTCTCATCAGCTTTGTTAGTTGCAATGAGTCACGCGTATGTTAAATAATATGTTTCTTTTCTTCTTA *F GTTACA *F 15559956 15560017 3L CG7325:1 61 *F TGTTGGGTAAGAGTTTTATTTTGTCAACGATTGTTTATATTTCTAACAACACATTTTTTTTTTGCAGTTCTTTGTA *F 4879335 4879392 2L CG3753:6 57 *F ACTTCAGTAAGATACATTTTGAATACTTTGAAAAATGTATTTTTCGCTAACTCAACTTTTTTTTTAGTCCCA *F 11133499 11133439 2R CG8435:3 60 *F AGCCCAGCACGATCAGTCTTTCCGCCACATCCTCCAGCAAAGCATCCGCAGCGCAGTCAATGGTTAAAAGGAAGA *F 12318997 12318935 3L CG4392:4 62 *F GATGTCGTGAGATGTCTGTCTTAATCTCGTTCCAATCAGGTTAACTCGGCTCGGCCCGGCCACCTCGGCTCTGCGGC *F 16353590 16353661 X CG18582:1 71 *F AAATCCACAGGCAAGAACATCCGCTTTAACTATTGTCAAAACCATTGAATTAATGGCATTTGGTTTTCTCATTACGTAC *F AGGTCGC *F 2825119 2825177 2R CG11217:3 58 *F ACTGTTTCAGGCAAGACCCGCGAACATTACGTTAAGCTCATTCCACTGAAGTGTTTCCCCTCTAAAAGGTTTT *F 5988569 5988513 2L CG9154:1 56 *F GGAGGATTGGGCAAGAGCACCTGCACATACCTTAGAAACTTATGGTGAAACTCTCTTCGCTTGCAGCAATT *F 3163265 3163210 2R CG8710:1 55 *F CTTCAGCTTGGCAAGATTTTTAAGAATCTCTAACTGCTAATTCTAAATAAATTGCATTCAAACAGAAGGA *F 19016796 19016717 2R CG4012:10 79 *F CTAGAACAAGGCAAGCAGCATTTAATGCACAGCACAGACTAGTCGGAGTTTTCCTAATGCATATATATATTATTATTCT *F GTGCTTTTAGTGGAT *F 5188869 5188929 2L CG14031:4 60 *F AATATTTGCGGCAAGCATAATCATCTATTTATATACGAAATTGTATTATTAACTATATTTTCTTCTATAGAAACT *F 8019903 8019849 2L CG8683:10 54 *F AATACAGAAGGCAAGCATTTACAAGACAATCCATTATAATATATAATTAATAATCTTTGAATAGGAGGC *F 1342668 1342597 3R CG2902:2 71 *F CACAATAAAGGCAAGCCCATTGACTAACTGGATGCTCCACTAATCCGAATGCGATAATCACGCTTTCCCATCCAACCGC *F AGCACCT *F 1921664 1921608 3L CG1960:11 56 *F GAAATGAAAGGCAAGCCTAACTAGAAATCTTATTCCAACTAGTGGCTAATCGGTAATCTCCCACAGTCATC *F 18784417 18784358 2R CG10327:4 59 *F GGACCAACAGGCAAGCCTTCACACCTCAATCATTGTTTCAAACAAAAAACTCTCTCACTTGCTCCGCAGGGCGG *F 10083374 10083316 3R CG8538:1 58 *F AGCTCGAAAGGCAAGCTTAGATATTTATCGAATCTCAAAGTGATACTCAGTTAATTCTCGCTTTTTAGAACCC *F 5140899 5140959 X CG4208:2 60 *F TGGCATACAGGCAAGGAAATTCTTTTGATTGCATCACAACTCGATTTAAACCCATCCCTTCTGCTTTCAGAATCC *F 10558265 10558328 3L CG18628:1 63 *F ATGGCCTCTGGCAAGGATTGCAATGGGTTACGCTCGACAACAGCTCTACTTAACTGCTTCCCATACATTCTAGCCCTT *F 14980446 14980449 2L CG15270:18 3 GGATAATTAAGCTAATAA *F 11800598 11800602 X CG1786:1 4 TTGCCCTTTGGGCATGCGG *F 21110150 21110155 3L CG18023:4 5 GCAGCCGCATGTAAGCGGCG *F 8109104 8109109 X CG2156:3 5 TTTCGTTGGTGTAAGGAACT *F 1864866 1864865 X CG3895:4 1 CGAGTATCAAGTAATG *F 11794818 11794822 2L CG18665:1 4 CCTGCGTAGAGTAGAGTGC *F 94543 94539 2R CG17478:1 4 CTACACCGGGGTAGATTTT *F 5454509 5454523 3R CG8348:1 14 AAATATGTATGTATTTCGTTCTAGGATGA *F 8169409 8169427 3R CG12242:1 18 GCCCGAGTTCGTCAAGCTCAATCCATAGCACAC *F 15298696 15298677 X CG18620:2 19 CGATCCCCTGGTCCAAGCTATTGATAAAGCACCC *F 15470950 15470977 X CG8184:13 27 GGCCCTGCCGGTGACGCCATCGAAACCGCGTCGAAAGATCTA *F 312955 312961 2L CG4574:2 6 CCAAAAATTTGTGAGGATGAT *F 11533103 11533083 3L CG18331:2 20 TCTTCCAATGGTGATGGAAACTCTACCCAGTCCTC *F 550344 550333 2R CG10453:3 11 CAGTTCACAGGTGCCGATCAGAGAAA *F 16969140 16969122 3R CG3353:2 18 ACGGTGCAAGGTGCGCTACTGCTCCGAGGACTG *F 8575767 8575782 2L CG18405:21 15 ACAAAGTTAAGTGCGGCGGCAGCAGCCATG *F 5673571 5673595 3L CG5406:22 24 GGTGGGCGGCGTGGGAGGCGTGGTCGTGGGCGGACTGGG *F 11896105 11896091 2L CG18787:2 14 AATCCTTTCGGTGGTTTCCAACAGCAGCA *F 22827234 22827205 3R CG6124:5 29 GTAAGGACGGGTGTGTCAACGGGCTTTGCGTTTCTCCAGATTTT *F 17439079 17439107 3L CG7555:11 28 ACCATACCAGGTTAATATCATCCGCTATGCGCTAGCAGCACCC *F 12037111 12037097 3L CG5654:3 14 GTTTTCCCATGTTCAAATTCCTAGAACGG *F 18311420 18311404 2R CG3487:1 16 TATTTGTGAGGTTCGTATGTTCGTCTGCCGC *F 22753922 22753902 3R CG6277:1 20 ACTACCATCAGTTGTGGGCTCCGCTACGTTATGAA *F 19890208 19890165 3R CG5501:10 43 *F TGGACGAAGGTAACAGCTGCTTATTTTTTTAAAAATTCAAAATAATAATTAAGGGGTA *F 16131207 16131268 3L CG5057:1 61 *F CCAGAAAATGTAAGAAAATGCTAAAATTTATTTGATCAAATGTCTATAAATATGTGCACCATTAGTAACAGCTTGG *F 21127774 21127714 3L CG7758:2 60 *F ATGAGAAGGGTAAGATTTGCGTGAAGACCCCTTTTTAACTTCACTTGAAACACGTACTTTTCTTTTACAGGCTGG *F 3245833 3245867 2R CG8706:1 34 GAACTGTAAGTAAGCCGCGGGCCTGGCCACGCCACCATGCCACCATGCC *F 4381249 4381299 2L CG15436:1 50 *F ATACATCAGGTAAGTAATATAGTCCTTCATAAAGTTCAGCCTAATAAGTATTGTTTATTACAGGG *F 16555316 16555257 2L CG17332:4 59 *F CGGCAAGCAGTAAGTTTAGATTTTATACTATGTTACATACAATCCAATTACCAAATCATTTTCTTCTAGCGTCT *F 13488963 13488934 X CG11197:5 29 AAAAAAAGGCTAATTGTATTTTTATAAATCTCCTTACAGTACGG *F 5349633 5349683 2R CG12908:9 50 *F ACTGGTAGGATACAATAATTGTTGGCCGAAAGCAAATGCCTAACCCAATATTCTCCTCAGATATT *F 7330922 7330957 3L CG18415:1 35 *F ATCGTAAGCATACCGCCTGAGGAAGCTAATAGGTTTATTGAAACTATGAG *F 325584 325528 3L CG6936:4 56 *F ACCAGCAGGTTAGATCATGTCGACGATACGGTGAAGAACATGTTCAAAACGTCTTTCTTAGGTTTTCTGGG *F 11486832 11486790 X CG2448:4 42 *F TTGACAAGAATATACATAAAGATATTTATATATATAAATAAATTATATATAGAAGTG *F 1500098 1500155 3R CG1347:2 57 *F GAAGCGGGAGTATCCCCGCCAGACAGCGGTAGCCAAGTCGAGGAGACAGTGAAAAACCAACGGCTCCATGAT *F 6992189 6992144 U CG17419:1 45 *F AATATGGAGGTATGAGTAATTTTAAATGGAAATTTTGCTAACTATATTTTCCAACAGGGT *F 5815239 5815304 3L CG5568:3 65 *F CATATTTAATTATTGTAATGTTTATGTTGTATTTGTATTTTTATTGTATTTGTATTTGTATTTGTTGTATTTATCGTAAG *F 1546419 1546459 2R CG9397:2 40 *F TTTGTATAAATATTTTTACTTCTCGTAACTCGATAGTCCGACCCACGAAAGTCGT *F 10289245 10289315 2L CG5096:1 70 *F CAAAGCTATGTCCAAGAAACATTTAACTATATGGTAATCATTAGTTATTTAGTTCGCTATATAATACACACTACTTGCA *F GGAAAA *F 7909097 7909173 2R CG3845:4 76 *F AATGAAAATTTCCCCAAGGGTAAGTGCTCAACATTTTAAATCTAATCTGTTTTATCGGCAACTTATATTTGCTTTTTTC *F TTCGTTAGGCAT *F 8026105 8026042 3R CG17645:2 63 *F GAAGTCATCATCCGGCGGAAAGCAGGCCGAGAAGAAGGGCAAGTACAGGATTGTGATGGTTCGTCATGGAGAGTCCGA *F 12661843 12661789 3L CG5433:5 54 *F AGTTTCAAAATCGAATCACTTAACTTAATTAACTTAAATTAATCATTCCCATTTATTCTTGCAGAAGAA *F 7332608 7332618 3L CG18415:3 10 TCCGTCGTAATCGGGCTGTAAATAA *F 4178850 4178915 X CG3626:3 65 *F AGCTTTCCCTTCTTTACCTATAAGGTGAGAGATTTCGAGATTCATGTTAATTTATATTAAATATTTTTTACCTACCTAAT *F 6049539 6049477 X CG3585:14 62 *F GACATTAAGGTGAATTTCAGTGGCGTAACTGCTCGTTGAGAACGTTTTTATTAATCAGTGTTTATCTCGCAGATCTG *F 16184194 16184269 2L CG4455:1 75 *F TGTTTTTAGGTGAGCGAAAGAGAGGGAGAAGAAATGAAGAAATCGCCTCGCGATCAGATTTTACGGATACGCATCTCGT *F TATTGCAGGCG *F 22636596 22636537 3L CG11352:6 59 *F ACGGCAAGGGTGAGTACTTCTGTGAGGCGTGTTTGGAGGCGGCTACCTTCTAATCAGATCCTCGTACAGGCCAA *F 25385908 25385974 3R CG18111:1 66 *F ATTGGACTGGTGAGTGCTCGATACAGATAAACGGCGACCAGGACCAGAATAATTCAACGCCTTCCCTTGGGTTATAGGC *F CT *F 15398624 15398680 3L CG7275:2 56 *F CAACGAGGATTGCAAGTGAGTAAAACGGCCATACGAAGAGGATGTGTTGCTAAAATCTTTATTTCTTACAG *F 4930913 4930855 2L CG18623:1 58 *F TGTAGCTCGGTGCCTGTGCGTGTGTGTGTGGGTGTGTCGTGTGCGTGCGTACGTGTGTGTGTGTGCGAAGTTC *F 15371470 15371419 3L CG7804:1 51 *F TACAACAATTTGGACAACAACTTTAAGATGCAACCGGCCAACAACTTTAGAATGCACCCAGCCAAC *F 26196079 26196151 3R CG18030:2 72 *F GTACACCCACTGGGTTGGAAGCGGAAACTTCGTCCAGCACCACCACTACAACAGCGGCAACCTGCACAACGACATCTCC *F CTGATCCG *F 8255588 8255516 2L CG14272:1 72 *F GGTTTAACTCTGGTCAAAAGAGAAACATTTAAATTTAAAATCTTGTATAACAATCGAGTTCCAAACAACGCCGTGTGCT *F CACCTCCG *F 25629490 25629529 3R CG15519:1 39 *F TTCTGGAGCCTGGTCAACAAGGTTGAGCTGCCCAGCTACAACGACCGCTACCAG *F 5182417 5182357 2R CG12215:5 60 *F TGGTGGGGTGTGGTGAGTCCCTTGGATCCCATGACATAATCACAGTGCTAGCCCATCCTTTCGATTCCAGATCAC *F 8482705 8482645 X CG12108:3 60 *F TCTATCAGGATGTACGTATGCATATCTATCACCTAACGTTCTAATATGAAATGTACATTTTTTCCTAAAGCTGGG *F 15558694 15558756 3L CG7327:2 62 *F AGCCTGTTTATGTGAGTGAACATCTCCAAAAGCTTAAGAAGGACCAAATATAATATTTTTCTAATTATTCAGCTACT *F 19629791 19629715 3R CG13598:9 76 *F TTTCTATGAGTGTGTGTGTGTGCCTTTCGTAGAGTCGCCCACCACAACGCAGCTGCTTTCATCAGTTCCAACCGCTCAA *F CCAACAGTCGAG *F 9493872 9493910 2R CG10117:1 38 *F CAGCTAAAAATGTTTTGTGTGTGTTTGTTTAATGTTTCTGTTGTTTAAGAGCG *F 17695439 17695388 3L CG5587:2 51 *F ACGAGGAGTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCACC *F 4455817 4455766 U CG17686:2 51 *F AACTATGGTTTTAAGTAGACCAGCCGACCACCCTGACGGTTCCGGGTAACTTGACGACTTGATGGT *F 18472254 18472201 3R CG4803:3 53 *F AGGCAAGAGATTATCTCTCGATATTATTATTATTATTATTATGTAATAAACTTATGATTTCAGGCGAG *F 24080681 24080748 3R CG1867:2 67 *F GAATGATTAATTCATACTATTAATTACCGACTTTCAACTAATATATTTATTAATTGCACTTCCTTTTTCACCCACAGAT *F TGT *F 2988737 2988681 X CG14265:1 56 *F ATGAAAAATATTCCAAAGGCACTTTCAATTTATTTGCGGTCTGGGGCAGCAAGACAATTTGGAAAGAAAAA *F 7675238 7675178 2L CG7179:1 60 *F GCAGCACCGATTCCTGCAGCAACAGCAATTACAACTGCAGATCCTGCAGCAACAGCGACTGCAACAGCAGGTCCT *F 6080218 6080173 3L CG10529:1 45 *F GTCGATATCATTCGCATCGAACTCTTCGAGATCTCCAAACCAGCCCAAATTCAACATGAA *F 6049677 6049624 X CG3585:13 53 *F AGGTGAGTTGTTCGGTTAGATATCGGTTTAAACGGGTGATAATCATGTTTAATTCCTCGACAGGCCCA *F 20951583 20951652 X CG1484:6 69 *F GGAGAACTTCTTCTGGGTGGCGCTGGGCGGCCGCAAGCCTTACGATACGGACGCCGAGTACATGAACTACACGCGGCTG *F TTCCG *F 1579401 1579420 X CG17968:1 19 CAAATTAATGTTGAACAACTAAAATCACAATGGA *F 3958248 3958180 2R CG8247:3 68 *F TCTCAGTGCCTTGTGCCAAAAGGTGGGTGTACATCTTCTGCAGTATCCCCTTATTTATTCCCCTCATTTGTGCTCTAGG *F TCAT *F 15151035 15151044 3R CG18493:1 9 AGTTGCCTCATTTGTGAAAATGGC *F 15141983 15141912 X CG9176:3 71 *F TTTTACGCGCTTTTTTAAAGTCAGTTAATCGTCGATGTTGAGCATTATTAATGTGCGTACTAATTTGACTGGCTTTTGC *F AGGTTTA *F \------------------------------------------------------------------------------ *F -- # *U FBrf0137492 *a Oliver *b B. *t 2001.8.16 *T personal communication to FlyBase *u File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). *F clone GenBank_Accn Assembled clone is in contig 'BE sub_id' 'BE % ids' 'BE strand' 'GF sub_id' 'GF % ids' 'GF strand' *F bs01a01.y1 AI944400 Contig134 LP11272.5prime 99% Plus / Plus CG1821|FBan0001821|CT5526|FBan0001821 99% Plus / Plus *F bs01a02.y1 AI944401 singleton LP01573.3prime 100% Plus / Plus CG5703|FBan0005703|CT17956|FBan0005703 100% Plus / Minus *F bs01a03.y1 AI944402 Contig533 GH21951.5prime 98% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs01a04.y1 AI944403 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 97% Plus / Plus *F bs01a05.y1 AI944404 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 96% Plus / Plus *F bs01a06.y1 AI944405 Contig453 GH22818.5prime 96% Plus / Plus CG9314|FBan0009314|CT26521|FBan0009314 99% Plus / Plus *F bs01a07.y1 pending singleton HL01494.5prime 95% Plus / Plus CG1271|FBan0001271|CT2614|FBan0001271 98% Plus / Plus *F bs01a08.y1 AI944406 Contig544 GH14656.5prime 98% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 98% Plus / Plus *F bs01a09.y1 AI944407 singleton CG7828|FBan0007828|CT23750|FBan0007828 99% Plus / Plus *F bs01a11.y1 AI944408 Contig402 LD11690.5prime 96% Plus / Plus CG3265|FBan0003265|CT37737|FBan0003265 99% Plus / Plus *F bs01a12.y1 AI944409 Contig456 GH23516.5prime 99% Plus / Plus CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs01b01.y1 AI944410 Contig480 GH15272.5prime 99% Plus / Plus CG1999|FBan0001999|CT6363|FBan0001999 99% Plus / Plus *F bs01b02.y1 AI944411 Contig544 GH22881.5prime 97% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 97% Plus / Plus *F bs01b03.y1 AI944412 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 97% Plus / Plus *F bs01b04.y1 AI944413 Contig538 GH26445.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs01b05.y1 AI944414 singleton GM06340.5prime 97% Plus / Plus CG1088|FBan0001088|CT1531|FBan0001088 97% Plus / Plus *F bs01b06.y1 AI944415 Contig170 GH14121.5prime 99% Plus / Plus CG8043|FBan0008043|CT24147|FBan0008043 99% Plus / Plus *F bs01b07.y1 AI944416 Contig308 GH22453.5prime 100% Plus / Plus CG5771|FBan0005771|CT18112|FBan0005771 100% Plus / Plus *F bs01b08.y1 pending singleton none none *F bs01b09.y1 AI944417 Contig530 GH13953.5prime 99% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs01b10.y1 AI944418 Contig451 GH08251.5prime 98% Plus / Plus CG18628|FBan0018628|CT41623|FBan0018628 98% Plus / Plus *F bs01b11.y1 AI944419 singleton GH10329.5prime 99% Plus / Plus CG5378|FBan0005378|CT17076|FBan0005378 99% Plus / Plus *F bs01b12.y1 AI944420 Contig460 GH10940.5prime 97% Plus / Minus CG18662|FBan0018662|CT42585|FBan0018662 97% Plus / Plus *F bs01c01.y1 AI944421 Contig401 LP07372.5prime 99% Plus / Minus none none *F bs01c02.y1 AI944422 Contig169 CG1690|FBan0001690|CT4772|FBan0001690 99% Plus / Plus *F bs01c03.y1 AI944423 Contig504 CG6372|FBan0006372|CT19794|FBan0006372 99% Plus / Plus *F bs01c04.y1 AI944424 singleton LP11867.5prime 99% Plus / Plus CG9916|FBan0009916|CT27926|FBan0009916 100% Plus / Plus *F bs01c05.y1 AI944425 singleton GH20936.5prime 99% Plus / Plus CG6670|FBan0006670|CT40842|FBan0006670 98% Plus / Plus *F bs01c06.y1 AI944426 singleton CG6263|FBan0006263|CT19243|FBan0006263 99% Plus / Plus *F bs01c07.y1 AI944427 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs01c08.y1 AI944428 Contig487 CG12470|FBan0012470|CT32706|FBan0012470 100% Plus / Plus *F bs01c09.y1 AI944429 Contig544 GM06241.5prime 100% Plus / Plus none none *F bs01c10.y1 AI944430 singleton GH15479.5prime 99% Plus / Plus CG9389|FBan0009389|CT26645|FBan0009389 99% Plus / Plus *F bs01c11.y1 AI944431 Contig478 LD33742.5prime 98% Plus / Plus none none *F bs01c12.y1 AI944432 Contig422 SD07466.5prime 99% Plus / Plus none none *F bs01d01.y1 AI944433 singleton LP03695.5prime 99% Plus / Plus CG11009|FBan0011009|CT30831|FBan0011009 99% Plus / Plus *F bs01d02.y1 AI944434 singleton CG14994|FBan0014994|CT34847|FBan0014994 100% Plus / Plus *F bs01d03.y1 AI944435 Contig168 CG4480|FBan0004480|CT14588|FBan0004480 96% Plus / Plus *F bs01d05.y1 AI944436 singleton CG10332|FBan0010332|CT29024|FBan0010332 99% Plus / Plus *F bs01d07.y1 AI944437 singleton CG18028|FBan0018028|CT40348|FBan0018028 96% Plus / Plus *F bs01d08.y1 AI944438 singleton none none *F bs01d09.y1 AI944439 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs01d10.y1 AI944440 Contig369 none none *F bs01d11.y1 AI944441 Contig167 GH25750.5prime 99% Plus / Plus CG14721|FBan0014721|CT34513|FBan0014721 99% Plus / Plus *F bs01d12.y1 AI944442 singleton CG18335|FBan0018335|CT41635|FBan0018335 97% Plus / Plus *F bs01e01.y1 AI944443 Contig532 GH16267.5prime 96% Plus / Plus CG17349|FBan0017349|CT32310|FBan0017349 96% Plus / Plus *F bs01e02.y1 AI944444 Contig544 CG11663|FBan0011663|CT34392|FBan0011663 98% Plus / Plus *F bs01e03.y1 AI944445 singleton CG9054|FBan0009054|CT25986|FBan0009054 99% Plus / Plus *F bs01e04.y1 AI944446 Contig166 CG10202|FBan0010202|CT28705|FBan0010202 100% Plus / Plus *F bs01e05.y1 AI944447 singleton CG9169|FBan0009169|CT8265|FBan0009169 94% Plus / Plus *F bs01e06.y1 AI944448 singleton GH03576.5prime 100% Plus / Plus CG6272|FBan0006272|CT19628|FBan0006272 100% Plus / Minus *F bs01e07.y1 AI944449 singleton CG12362|FBan0012362|CT24168|FBan0012362 97% Plus / Plus *F bs01e08.y1 AI944450 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs01e09.y1 AI944451 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 98% Plus / Plus *F bs01e10.y1 AI944452 Contig523 LD23336.3prime 100% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 100% Plus / Plus *F bs01e11.y1 pending Contig376 LP04452.5prime 100% Plus / Plus CG9920|FBan0009920|CT27932|FBan0009920 100% Plus / Plus *F bs01e12.y1 pending Contig250 CG17944|FBan0017944|CT39966|FBan0017944 98% Plus / Plus *F bs01f01.y1 AI944453 Contig544 CG3124|FBan0003124|CT10470|FBan0003124 100% Plus / Plus *F bs01f02.y1 AI944454 Contig544 CG5077|FBan0005077|CT16281|FBan0005077 99% Plus / Plus *F bs01f03.y1 AI944455 Contig400 SD04285.5prime 99% Plus / Minus none none *F bs01f04.y1 AI944456 Contig526 CG1324|FBan0001324|CT2936|FBan0001324 98% Plus / Plus *F bs01f05.y1 AI944457 singleton CG17375|FBan0017375|CT33259|FBan0017375 98% Plus / Plus *F bs01f06.y1 AI944458 Contig544 none none *F bs01f07.y1 AI944459 Contig540 LP07660.3prime 99% Plus / Plus CG17137|FBan0017137|CT38066|FBan0017137 99% Plus / Minus *F bs01f08.y1 AI944460 Contig163 GH27049.5prime 99% Plus / Plus CG5343|FBan0005343|CT16991|FBan0005343 98% Plus / Plus *F bs01f09.y1 AI944461 Contig323 LD06980.5prime 97% Plus / Plus CG9288|FBan0009288|CT26469|FBan0009288 97% Plus / Plus *F bs01f10.y1 AI944462 singleton GM01832.5prime 99% Plus / Plus none none *F bs01f11.y1 AI944463 Contig336 CG9632|FBan0009632|CT27234|FBan0009632 98% Plus / Plus *F bs01g01.y1 AI944464 Contig451 GH08251.5prime 100% Plus / Plus CG18628|FBan0018628|CT41623|FBan0018628 100% Plus / Plus *F bs01g02.y1 AI944465 Contig544 GH10815.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs01g03.y1 AI944466 Contig526 none none *F bs01g04.y1 AI944467 Contig160 none none *F bs01g05.y1 AI944468 Contig499 LP02092.5prime 98% Plus / Plus CG8053|FBan0008053|CT24166|FBan0008053 98% Plus / Plus *F bs01g06.y1 AI944469 Contig399 GH07182.5prime 99% Plus / Plus CG5217|FBan0005217|CT16693|FBan0005217 99% Plus / Plus *F bs01g07.y1 AI944470 Contig476 LP11595.5prime 98% Plus / Plus CG3752|FBan0003752|CT12541|FBan0003752 98% Plus / Plus *F bs01g08.y1 AI944471 Contig531 GH04958.5prime 99% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs01g09.y1 pending Contig170 GH14121.5prime 97% Plus / Plus CG8043|FBan0008043|CT24147|FBan0008043 97% Plus / Plus *F bs01g10.y1 AI944472 Contig526 CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs01g11.y1 AI944473 Contig527 none none *F bs01h01.y1 AI944474 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs01h02.y1 AI944475 singleton CG3964|FBan0003964|CT13163|FBan0003964 99% Plus / Plus *F bs01h03.y1 AI944476 singleton CG14017|FBan0014017|CT33574|FBan0014017 100% Plus / Plus *F bs01h04.y1 AI944477 Contig544 GH03736.5prime 99% Plus / Plus CG3124|FBan0003124|CT10470|FBan0003124 98% Plus / Plus *F bs01h05.y1 AI944478 Contig524 none none *F bs01h06.y1 AI944479 singleton CG18368|FBan0018368|CT41747|FBan0018368 98% Plus / Plus *F bs01h07.y1 pending singleton CG7394|FBan0007394|CT22766|FBan0007394 99% Plus / Plus *F bs01h08.y1 pending singleton CG9127|FBan0009127|CT26156|FBan0009127 99% Plus / Plus *F bs01h09.y1 AI944480 Contig159 GH27147.5prime 100% Plus / Plus CG3199|FBan0003199|CT10703|FBan0003199 100% Plus / Plus *F bs01h10.y1 AI944481 singleton LD26185.5prime 98% Plus / Plus CG16788|FBan0016788|CT32104|FBan0016788 98% Plus / Plus *F bs01h11.y1 AI944482 Contig449 CG9218|FBan0009218|CT26312|FBan0009218 99% Plus / Plus *F bs01h12.y1 pending Contig518 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 98% Plus / Plus *F bs02a01.y1 AI944483 singleton CG6527|FBan0006527|CT20307|FBan0006527 93% Plus / Plus *F bs02a02.y1 AI944484 Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs02a03.y1 AI944485 Contig157 none none *F bs02a04.y1 pending Contig538 GH15626.5prime 96% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 96% Plus / Plus *F bs02a05.y1 AI944486 Contig544 GH26094.5prime 98% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 98% Plus / Plus *F bs02a06.y1 AI944487 Contig398 CG11588|FBan0011588|CT22407|FBan0011588 98% Plus / Plus *F bs02a07.y1 AI944488 Contig454 CG2164|FBan0002164|CT7072|FBan0002164 98% Plus / Plus *F bs02a08.y1 pending singleton GM03239.5prime 97% Plus / Plus CG3412|FBan0003412|CT11401|FBan0003412 97% Plus / Plus *F bs02a09.y1 AI944489 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs02a10.y1 AI944490 singleton LD29067.5prime 97% Plus / Plus CG6148|FBan0006148|CT19156|FBan0006148 98% Plus / Plus *F bs02a11.y1 AI944491 Contig522 CG13414|FBan0013414|CT32770|FBan0013414 99% Plus / Plus *F bs02a12.y1 AI944492 singleton none none *F bs02b01.y1 AI944570 singleton CG7551|FBan0007551|CT23115|FBan0007551 99% Plus / Plus *F bs02b02.y1 AI944571 singleton GH20274.5prime 95% Plus / Plus none none *F bs02b03.y1 AI944572 singleton CG3123|FBan0003123|CT10492|FBan0003123 99% Plus / Plus *F bs02b04.y1 AI944573 Contig475 CG7931|FBan0007931|CT6483|FBan0007931 97% Plus / Minus *F bs02b05.y1 pending Contig512 LP07747.5prime 96% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 96% Plus / Plus *F bs02b06.y1 AI944574 Contig539 GH27943.3prime 97% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 97% Plus / Plus *F bs02b07.y1 AI944575 Contig156 CG9922|FBan0009922|CT27936|FBan0009922 100% Plus / Plus *F bs02b08.y1 AI944576 Contig459 GH07093.5prime 99% Plus / Plus CG4323|FBan0004323|CT14141|FBan0004323 99% Plus / Plus *F bs02b09.y1 AI944577 Contig154 SD06593.5prime 99% Plus / Plus CG1728|FBan0001728|CT4964|FBan0001728 99% Plus / Plus *F bs02b10.y1 pending Contig223 CG9966|FBan0009966|CT28103|FBan0009966 98% Plus / Plus *F bs02b11.y1 AI944578 singleton CG1988|FBan0001988|CT6344|FBan0001988 100% Plus / Plus *F bs02b12.y1 AI944579 Contig307 LP07781.5prime 98% Plus / Plus CG17819|FBan0017819|CT39518|FBan0017819 98% Plus / Plus *F bs02c01.y1 AI944580 singleton none none *F bs02c02.y1 AI944581 Contig520 none none *F bs02c03.y1 AI944582 Contig514 GH24664.5prime 100% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 100% Plus / Plus *F bs02c04.y1 AI944583 singleton CG12169|FBan0012169|CT8905|FBan0012169 99% Plus / Plus *F bs02c05.y1 AI944584 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs02c06.y1 AI944585 Contig306 GM04388.5prime 97% Plus / Plus CG10191|FBan0010191|CT28607|FBan0010191 98% Plus / Plus *F bs02c07.y1 pending Contig388 LP11254.5prime 97% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 97% Plus / Plus *F bs02c08.y1 AI944586 Contig305 CG12377|FBan0012377|CT25244|FBan0012377 97% Plus / Plus *F bs02c10.y1 AI944587 Contig440 GH04279.5prime 99% Plus / Plus CG13030|FBan0013030|CT32248|FBan0013030 99% Plus / Plus *F bs02c11.y1 AI944588 singleton GH08727.5prime 99% Plus / Plus CG4464|FBan0004464|CT14524|FBan0004464 99% Plus / Plus *F bs02c12.y1 AI944589 singleton CG3575|FBan0003575|CT12015|FBan0003575 99% Plus / Plus *F bs02d01.y1 pending singleton LP03431.5prime 98% Plus / Plus CG7766|FBan0007766|CT23171|FBan0007766 98% Plus / Plus *F bs02d02.y1 AI944590 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 98% Plus / Plus *F bs02d03.y1 AI944591 Contig455 CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs02d04.y1 pending Contig457 GH18334.5prime 97% Plus / Plus CG6255|FBan0006255|CT19574|FBan0006255 97% Plus / Plus *F bs02d05.y1 pending singleton GH08003.5prime 98% Plus / Plus CG14665|FBan0014665|CT34446|FBan0014665 99% Plus / Plus *F bs02d06.y1 AI944592 singleton GH05159.5prime 99% Plus / Plus CG16964|FBan0016964|CT37639|FBan0016964 99% Plus / Plus *F bs02d07.y1 AI944593 singleton LD07441.5prime 96% Plus / Minus CG10255|FBan0010255|CT28815|FBan0010255 98% Plus / Plus *F bs02d08.y1 AI944594 Contig304 CG16984|FBan0016984|CT34790|FBan0016984 97% Plus / Plus *F bs02d10.y1 AI944595 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs02d11.y1 AI944596 Contig542 GH21096.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs02d12.y1 AI944597 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs02e01.y1 AI944598 Contig446 LP09632.5prime 99% Plus / Plus CG3691|FBan0003691|CT12381|FBan0003691 99% Plus / Plus *F bs02e02.y1 AI944599 singleton none none *F bs02e03.y1 AI944600 Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 98% Plus / Plus *F bs02e04.y1 AI944601 singleton CG13661|FBan0013661|CT33090|FBan0013661 97% Plus / Plus *F bs02e05.y1 AI944602 Contig538 GH09790.5prime 99% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 99% Plus / Plus *F bs02e06.y1 AI944603 Contig461 GH03304.5prime 94% Plus / Plus CG6372|FBan0006372|CT19794|FBan0006372 94% Plus / Plus *F bs02e07.y1 AI944604 Contig453 CG9314|FBan0009314|CT26521|FBan0009314 98% Plus / Plus *F bs02e08.y1 AI944605 singleton GH22555.5prime 99% Plus / Plus CG8806|FBan0008806|CT8669|FBan0008806 99% Plus / Plus *F bs02e09.y1 AI944606 Contig397 CG11861|FBan0011861|CT40006|FBan0011861 99% Plus / Plus *F bs02e10.y1 AI944607 Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 98% Plus / Plus *F bs02e11.y1 AI944608 singleton LP12042.5prime 99% Plus / Plus CG5537|FBan0005537|CT17516|FBan0005537 99% Plus / Plus *F bs02e12.y1 AI944609 singleton LD43584.5prime 99% Plus / Plus CG4832|FBan0004832|CT15503|FBan0004832 99% Plus / Plus *F bs02f01.y1 pending Contig544 GH06583.5prime 99% Plus / Plus CG9029|FBan0009029|CT25936|FBan0009029 99% Plus / Plus *F bs02f02.y1 pending Contig372 GH13257.5prime 98% Plus / Plus CG17010|FBan0017010|CT37753|FBan0017010 98% Plus / Plus *F bs02f03.y1 AI944610 singleton GH18718.5prime 94% Plus / Plus CG1101|FBan0001101|CT1611|FBan0001101 97% Plus / Plus *F bs02f04.y1 pending singleton GH05976.5prime 98% Plus / Plus CG3109|FBan0003109|CT10436|FBan0003109 98% Plus / Plus *F bs02f05.y1 AI944611 Contig511 GH22851.5prime 96% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 96% Plus / Plus *F bs02f06.y1 AI944612 Contig544 none none *F bs02f07.y1 AI944613 singleton GH19032.5prime 96% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 96% Plus / Plus *F bs02f08.y1 AI944614 Contig303 HL03878.5prime 98% Plus / Plus CG10089|FBan0010089|CT28377|FBan0010089 98% Plus / Plus *F bs02f09.y1 AI944615 Contig405 none none *F bs02f10.y1 AI944616 singleton GH10940.5prime 98% Plus / Minus CG18662|FBan0018662|CT42585|FBan0018662 98% Plus / Plus *F bs02f11.y1 pending singleton none none *F bs02f12.y1 AI944617 Contig510 GH21762.5prime 99% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 99% Plus / Plus *F bs02g01.y1 pending Contig302 GH21955.5prime 97% Plus / Plus CG10652|FBan0010652|CT29824|FBan0010652 98% Plus / Plus *F bs02g02.y1 AI944618 Contig512 LP07747.5prime 97% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 97% Plus / Plus *F bs02g03.y1 AI944619 singleton CG12881|FBan0012881|CT32025|FBan0012881 100% Plus / Plus *F bs02g04.y1 pending Contig527 none none *F bs02g05.y1 AI944620 Contig410 CG17946|FBan0017946|CT39984|FBan0017946 95% Plus / Plus *F bs02g06.y1 AI944621 singleton CG4712|FBan0004712|CT15189|FBan0004712 99% Plus / Plus *F bs02g07.y1 AI944622 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs02g08.y1 AI944623 singleton GH13310.5prime 100% Plus / Plus CG10423|FBan0010423|CT29278|FBan0010423 100% Plus / Plus *F bs02g09.y1 AI944624 Contig538 GH26445.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs02g10.y1 AI944625 singleton CG16973|FBan0016973|CT37669|FBan0016973 98% Plus / Plus *F bs02g11.y1 AI944626 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs02g12.y1 AI944627 Contig468 CG13898|FBan0013898|CT33433|FBan0013898 100% Plus / Plus *F bs02h01.y1 AI944628 Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs02h02.y1 AI944629 singleton LD38527.5prime 100% Plus / Plus CG3510|FBan0003510|CT11825|FBan0003510 100% Plus / Plus *F bs02h03.y1 AI944630 singleton CG1980|FBan0001980|CT6205|FBan0001980 97% Plus / Plus *F bs02h04.y1 AI944631 Contig544 GH14656.5prime 97% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 97% Plus / Plus *F bs02h05.y1 AI944632 Contig301 LP10167.3prime 99% Plus / Minus none none *F bs02h06.y1 AI944633 singleton none none *F bs02h07.y1 AI944634 Contig409 none none *F bs02h08.y1 AI944635 Contig300 CG9592|FBan0009592|CT27112|FBan0009592 98% Plus / Plus *F bs02h09.y1 AI944636 singleton LD24014.3prime 100% Plus / Minus CG1109|FBan0001109|CT1643|FBan0001109 99% Plus / Plus *F bs02h10.y1 AI944637 Contig518 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 98% Plus / Plus *F bs02h11.y1 AI944638 Contig450 GH23475.5prime 99% Plus / Plus none none *F bs02h12.y1 AI944639 singleton LD12532.5prime 100% Plus / Plus CG4802|FBan0004802|CT15435|FBan0004802 100% Plus / Plus *F bs03a01.y1 AI944640 Contig536 LP09196.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs03a02.y1 AI944641 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 100% Plus / Plus *F bs03a04.y1 AI944642 Contig452 GH26627.5prime 100% Plus / Plus CG3085|FBan0003085|CT10370|FBan0003085 100% Plus / Plus *F bs03a05.y1 AI944643 Contig544 none none *F bs03a06.y1 AI944644 Contig299 CG6262|FBan0006262|CT42653|FBan0006262 99% Plus / Plus *F bs03a07.y1 AI944645 Contig544 CG5790|FBan0005790|CT18162|FBan0005790 98% Plus / Plus *F bs03a08.y1 AI944646 Contig341 LD21602.3prime 97% Plus / Minus CG6412|FBan0006412|CT19988|FBan0006412 99% Plus / Plus *F bs03a09.y1 AI944647 Contig298 CG15711|FBan0015711|CT35936|FBan0015711 99% Plus / Plus *F bs03a10.y1 AI944648 singleton GH04888.5prime 96% Plus / Plus CG8525|FBan0008525|CT24905|FBan0008525 97% Plus / Plus *F bs03a11.y1 AI944649 Contig396 CG11392|FBan0011392|CT31794|FBan0011392 100% Plus / Plus *F bs03a12.y1 AI944650 Contig543 CG4750|FBan0004750|CT15217|FBan0004750 97% Plus / Plus *F bs03b01.y1 AI944651 Contig481 CG5641|FBan0005641|CT17812|FBan0005641 100% Plus / Plus *F bs03b02.y1 AI944652 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs03b03.y1 AI944653 Contig538 GH09790.5prime 100% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 100% Plus / Plus *F bs03b04.y1 AI944654 singleton CG4706|FBan0004706|CT15177|FBan0004706 100% Plus / Plus *F bs03b06.y1 AI944655 Contig297 GH08590.5prime 100% Plus / Plus CG3517|FBan0003517|CT11863|FBan0003517 100% Plus / Plus *F bs03b07.y1 AI944656 Contig470 GH25094.5prime 99% Plus / Plus CG17450|FBan0017450|CT34184|FBan0017450 99% Plus / Plus *F bs03b08.y1 AI944657 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs03b09.y1 AI944493 singleton CG6752|FBan0006752|CT20907|FBan0006752 97% Plus / Plus *F bs03b10.y1 AI944494 Contig511 GH15075.5prime 100% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 100% Plus / Plus *F bs03b11.y1 AI944495 Contig296 CG13394|FBan0013394|CT32740|FBan0013394 98% Plus / Plus *F bs03b12.y1 AI944496 singleton LD43045.5prime 100% Plus / Plus CG11856|FBan0011856|CT33077|FBan0011856 100% Plus / Plus *F bs03c01.y1 AI944497 Contig295 CG3492|FBan0003492|CT11747|FBan0003492 99% Plus / Plus *F bs03c02.y1 AI944498 singleton GM02783.5prime 96% Plus / Plus CG6859|FBan0006859|CT21249|FBan0006859 98% Plus / Plus *F bs03c03.y1 AI944499 Contig485 none none *F bs03c04.y1 AI944500 Contig507 GM04405.5prime 100% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs03c05.y1 AI944501 singleton CG7408|FBan0007408|CT22813|FBan0007408 100% Plus / Plus *F bs03c06.y1 AI944502 Contig544 GH22881.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs03c07.y1 pending Contig293 GH19824.5prime 95% Plus / Plus CG2277|FBan0002277|CT7561|FBan0002277 95% Plus / Plus *F bs03c08.y1 AI944503 Contig292 CG8937|FBan0008937|CT25664|FBan0008937 98% Plus / Plus *F bs03c09.y1 AI944504 Contig528 CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs03c10.y1 AI944505 singleton none none *F bs03c11.y1 AI944506 singleton GH12504.3prime 100% Plus / Minus CG9808|FBan0009808|CT27712|FBan0009808 100% Plus / Plus *F bs03c12.y1 AI944507 Contig291 GH15129.5prime 98% Plus / Minus CG4523|FBan0004523|CT14670|FBan0004523 99% Plus / Plus *F bs03d01.y1 AI944508 Contig459 GH07093.5prime 100% Plus / Plus CG4323|FBan0004323|CT14141|FBan0004323 100% Plus / Plus *F bs03d02.y1 AI944509 singleton GH22517.5prime 100% Plus / Plus CG5119|FBan0005119|CT39088|FBan0005119 100% Plus / Plus *F bs03d03.y1 AI944510 singleton CG7962|FBan0007962|CT23976|FBan0007962 100% Plus / Plus *F bs03d04.y1 pending Contig290 none none *F bs03d05.y1 AI944511 Contig395 LD07629.5prime 99% Plus / Plus CG8472|FBan0008472|CT41659|FBan0008472 99% Plus / Plus *F bs03d06.y1 AI944512 Contig394 CG7059|FBan0007059|CT21831|FBan0007059 99% Plus / Plus *F bs03d07.y1 AI944513 Contig289 GM02886.5prime 99% Plus / Plus CG6692|FBan0006692|CT20780|FBan0006692 97% Plus / Plus *F bs03d08.y1 AI944514 Contig393 GH28709.5prime 100% Plus / Plus CG18427|FBan0018427|CT30112|FBan0018427 100% Plus / Plus *F bs03d09.y1 AI944515 singleton CK01043.contig 98% Plus / Minus CG15828|FBan0015828|CT39214|FBan0015828 100% Plus / Plus *F bs03d10.y1 AI944516 singleton SD04124.5prime 100% Plus / Minus CG1618|FBan0001618|CT41661|FBan0001618 99% Plus / Plus *F bs03d11.y1 AI944517 singleton CG4690|FBan0004690|CT15131|FBan0004690 99% Plus / Minus *F bs03d12.y1 AI944518 singleton CG12635|FBan0012635|CT35210|FBan0012635 99% Plus / Plus *F bs03e01.y1 AI944519 Contig544 CG6709|FBan0006709|CT20844|FBan0006709 98% Plus / Plus *F bs03e02.y1 AI944520 singleton LD28208.5prime 100% Plus / Plus CG11154|FBan0011154|CT31172|FBan0011154 100% Plus / Plus *F bs03e03.y1 AI944521 singleton CG4724|FBan0004724|CT15179|FBan0004724 99% Plus / Plus *F bs03e04.y1 AI944522 Contig542 GH21803.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs03e05.y1 AI944523 singleton CG3107|FBan0003107|CT10228|FBan0003107 96% Plus / Minus *F bs03e06.y1 AI944524 Contig288 GH27684.5prime 99% Plus / Plus CG1655|FBan0001655|CT4502|FBan0001655 99% Plus / Plus *F bs03e07.y1 AI944525 Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs03e08.y1 AI944526 Contig451 CG15434|FBan0015434|CT35498|FBan0015434 95% Plus / Plus *F bs03e09.y1 AI944527 Contig482 CG18449|FBan0018449|CT42026|FBan0018449 99% Plus / Plus *F bs03e10.y1 AI944528 Contig322 GH13015.5prime 100% Plus / Plus CG6306|FBan0006306|CT19682|FBan0006306 100% Plus / Plus *F bs03e11.y1 AI944529 Contig532 LP10167.3prime 99% Plus / Minus CG8040|FBan0008040|CT24130|FBan0008040 100% Plus / Plus *F bs03e12.y1 AI944530 Contig497 GH27078.5prime 100% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 100% Plus / Plus *F bs03f01.y1 AI944531 Contig514 GH04665.5prime 94% Plus / Plus none none *F bs03f02.y1 AI944532 Contig287 GH05818.5prime 99% Plus / Plus CG17666|FBan0017666|CT39001|FBan0017666 99% Plus / Plus *F bs03f03.y1 AI944533 Contig401 LP06294.5prime 99% Plus / Minus CG4644|FBan0004644|CT14999|FBan0004644 100% Plus / Plus *F bs03f04.y1 AI944534 Contig286 GH23889.5prime 95% Plus / Plus CG5207|FBan0005207|CT16475|FBan0005207 100% Plus / Plus *F bs03f05.y1 AI944535 singleton none none *F bs03f06.y1 AI944536 Contig284 LD11861.5prime 100% Plus / Minus CG15233|FBan0015233|CT35170|FBan0015233 100% Plus / Minus *F bs03f07.y1 AI944537 singleton GH09161.5prime 97% Plus / Plus CG8508|FBan0008508|CT24847|FBan0008508 100% Plus / Plus *F bs03f08.y1 AI944538 Contig283 CG16751|FBan0016751|CT37261|FBan0016751 100% Plus / Plus *F bs03f09.y1 AI944539 singleton CG4961|FBan0004961|CT15711|FBan0004961 100% Plus / Plus *F bs03f10.y1 AI944540 Contig544 GH08269.3prime 99% Plus / Minus none none *F bs03f11.y1 AI944541 singleton none none *F bs03f12.y1 AI944542 singleton CG8230|FBan0008230|CT8599|FBan0008230 99% Plus / Plus *F bs03g01.y1 AI944543 singleton CG11815|FBan0011815|CT33479|FBan0011815 99% Plus / Minus *F bs03g02.y1 AI944544 singleton none none *F bs03g03.y1 AI944545 Contig447 GH12486.5prime 95% Plus / Plus CG4375|FBan0004375|CT14270|FBan0004375 95% Plus / Plus *F bs03g04.y1 AI944546 Contig392 GH10365.5prime 100% Plus / Plus CG17238|FBan0017238|CT21085|FBan0017238 100% Plus / Plus *F bs03g05.y1 AI944547 singleton CG7497|FBan0007497|CT23019|FBan0007497 100% Plus / Plus *F bs03g06.y1 AI944548 Contig544 GH16332.5prime 99% Plus / Plus CG18111|FBan0018111|CT40745|FBan0018111 99% Plus / Plus *F bs03g07.y1 AI944549 singleton CG7639|FBan0007639|CT23097|FBan0007639 100% Plus / Plus *F bs03g08.y1 AI944550 Contig513 CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Minus *F bs03g09.y1 AI944551 singleton GH27163.5prime 99% Plus / Plus CG7570|FBan0007570|CT23169|FBan0007570 99% Plus / Plus *F bs03g10.y1 AI944552 Contig483 CG15200|FBan0015200|CT35127|FBan0015200 100% Plus / Plus *F bs03g12.y1 AI944553 singleton LD34885.5prime 100% Plus / Plus CG3582|FBan0003582|CT11994|FBan0003582 100% Plus / Plus *F bs03h01.y1 AI944554 Contig503 GH11521.5prime 100% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 100% Plus / Plus *F bs03h02.y1 AI944555 Contig519 GH05452.5prime 99% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs03h03.y1 AI944556 Contig281 none none *F bs03h04.y1 AI944557 singleton CG14039|FBan0014039|CT33598|FBan0014039 99% Plus / Plus *F bs03h05.y1 AI944558 singleton HL04251.5prime 98% Plus / Plus CG15825|FBan0015825|CT38769|FBan0015825 99% Plus / Plus *F bs03h06.y1 AI944658 Contig391 CG4270|FBan0004270|CT37757|FBan0004270 99% Plus / Plus *F bs03h07.y1 AI944659 Contig280 CG14738|FBan0014738|CT34531|FBan0014738 100% Plus / Plus *F bs03h08.y1 AI944660 Contig528 CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs03h09.y1 AI944661 Contig279 GH22225.3prime 100% Plus / Minus CG3581|FBan0003581|CT12033|FBan0003581 100% Plus / Plus *F bs03h10.y1 AI944662 Contig411 CG11125|FBan0011125|CT8285|FBan0011125 100% Plus / Plus *F bs03h11.y1 AI944663 Contig540 GH14327.3prime 98% Plus / Minus CG7188|FBan0007188|CT22197|FBan0007188 98% Plus / Plus *F bs03h12.y1 AI944664 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs04a01.y1 AI944665 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs04a02.y1 AI944666 singleton GH26973.5prime 99% Plus / Plus CG2210|FBan0002210|CT7286|FBan0002210 99% Plus / Plus *F bs04a03.y1 AI944667 Contig524 GH18034.5prime 99% Plus / Plus CG9391|FBan0009391|CT26653|FBan0009391 99% Plus / Plus *F bs04a04.y1 AI944668 Contig417 LP10240.5prime 97% Plus / Plus CG2955|FBan0002955|CT10021|FBan0002955 96% Plus / Plus *F bs04a05.y1 AI944669 singleton SD05439.5prime 99% Plus / Plus CG10512|FBan0010512|CT29490|FBan0010512 99% Plus / Plus *F bs04a06.y1 AI944670 singleton CG5085|FBan0005085|CT16319|FBan0005085 100% Plus / Plus *F bs04a07.y1 AI944671 Contig393 GH28709.5prime 100% Plus / Plus CG18427|FBan0018427|CT30112|FBan0018427 99% Plus / Plus *F bs04a09.y1 AI944672 Contig277 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs04a10.y1 AI944673 Contig529 CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs04a11.y1 AI944674 Contig527 none none *F bs04a12.y1 pending Contig276 LP06848.5prime 94% Plus / Plus CG17462|FBan0017462|CT31210|FBan0017462 94% Plus / Plus *F bs04b01.y1 AI944675 Contig429 CG6380|FBan0006380|CT19908|FBan0006380 99% Plus / Plus *F bs04b02.y1 pending Contig390 GH24571.5prime 99% Plus / Plus CG9975|FBan0009975|CT28093|FBan0009975 99% Plus / Plus *F bs04b03.y1 AI944676 Contig538 GH26445.5prime 95% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 95% Plus / Plus *F bs04b04.y1 AI944677 Contig490 LD20282.5prime 98% Plus / Plus CG6584|FBan0006584|CT20478|FBan0006584 99% Plus / Plus *F bs04b05.y1 AI944678 singleton CG8013|FBan0008013|CT24080|FBan0008013 100% Plus / Plus *F bs04b06.y1 AI944679 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs04b07.y1 AI944680 singleton CK00496.3prime 98% Plus / Plus CG10576|FBan0010576|CT29664|FBan0010576 98% Plus / Minus *F bs04b08.y1 AI944681 Contig544 GH25863.5prime 99% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 99% Plus / Plus *F bs04b09.y1 AI944682 Contig544 LD21906.3prime 97% Plus / Minus CG5486|FBan0005486|CT17382|FBan0005486 99% Plus / Plus *F bs04b10.y1 AI944683 singleton GH02722.5prime 99% Plus / Plus CG12123|FBan0012123|CT7484|FBan0012123 99% Plus / Plus *F bs04b11.y1 AI944684 Contig437 CG3387|FBan0003387|CT11303|FBan0003387 98% Plus / Plus *F bs04b12.y1 AI944685 singleton LD02855.5prime 99% Plus / Plus CG4027|FBan0004027|CT13368|FBan0004027 99% Plus / Plus *F bs04c01.y1 AI944686 Contig510 LD39710.5prime 99% Plus / Minus CG10159|FBan0010159|CT28573|FBan0010159 99% Plus / Plus *F bs04c02.y1 AI944687 singleton CG6100|FBan0006100|CT19175|FBan0006100 99% Plus / Plus *F bs04c03.y1 AI944688 singleton GH09529.5prime 100% Plus / Plus CG9712|FBan0009712|CT27462|FBan0009712 100% Plus / Plus *F bs04c04.y1 AI944689 singleton GH14639.3prime 100% Plus / Minus CG5469|FBan0005469|CT42571|FBan0005469 100% Plus / Plus *F bs04c05.y1 AI944690 singleton CG1660|FBan0001660|CT4478|FBan0001660 100% Plus / Plus *F bs04c06.y1 AI944691 singleton CG6138|FBan0006138|CT19270|FBan0006138 99% Plus / Plus *F bs04c07.y1 AI944692 Contig468 GH13002.5prime 98% Plus / Plus CG13918|FBan0013918|CT33457|FBan0013918 99% Plus / Plus *F bs04c08.y1 pending singleton none none *F bs04c09.y1 AI944693 Contig524 CG6914|FBan0006914|CT21294|FBan0006914 100% Plus / Plus *F bs04c10.y1 AI944694 singleton LD33652.5prime 99% Plus / Plus CG12276|FBan0012276|CT17770|FBan0012276 99% Plus / Plus *F bs04c11.y1 AI944695 singleton LD13919.5prime 99% Plus / Plus CG10538|FBan0010538|CT29559|FBan0010538 99% Plus / Plus *F bs04c12.y1 AI944696 Contig477 LP11903.5prime 81% Plus / Plus CG12334|FBan0012334|CT22897|FBan0012334 99% Plus / Plus *F bs04d01.y1 AI944697 Contig275 GM14316.5prime 98% Plus / Plus CG5684|FBan0005684|CT17938|FBan0005684 99% Plus / Plus *F bs04d02.y1 AI944698 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs04d03.y1 AI944699 singleton GH12489.5prime 98% Plus / Minus CG11534|FBan0011534|CT36427|FBan0011534 99% Plus / Minus *F bs04d04.y1 AI944700 Contig389 GH05725.5prime 92% Plus / Plus CG18078|FBan0018078|CT40551|FBan0018078 99% Plus / Plus *F bs04d05.y1 AI944701 singleton CG11196|FBan0011196|CT31268|FBan0011196 100% Plus / Plus *F bs04d06.y1 AI944702 Contig465 CG4434|FBan0004434|CT14426|FBan0004434 99% Plus / Plus *F bs04d07.y1 AI944703 Contig524 LD45537.5prime 98% Plus / Minus CG3365|FBan0003365|CT11203|FBan0003365 100% Plus / Minus *F bs04d08.y1 AI944704 Contig544 GH22513.5prime 96% Plus / Plus none none *F bs04d09.y1 AI944705 Contig388 LP11254.5prime 100% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs04d10.y1 AI944706 Contig528 GH06435.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs04d11.y1 AI944707 Contig544 GH22881.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs04d12.y1 AI944708 Contig544 none none *F bs04e01.y1 AI944709 singleton CG2794|FBan0002794|CT9525|FBan0002794 98% Plus / Plus *F bs04e04.y1 AI944710 Contig470 CG17450|FBan0017450|CT34184|FBan0017450 99% Plus / Plus *F bs04e05.y1 AI944711 Contig274 LD01152.5prime 95% Plus / Plus CG7701|FBan0007701|CT23463|FBan0007701 98% Plus / Plus *F bs04e06.y1 AI944712 singleton none none *F bs04e07.y1 AI944713 Contig544 GH20547.5prime 99% Plus / Plus none none *F bs04e08.y1 AI944714 Contig273 GM13788.3prime 100% Plus / Minus CG8542|FBan0008542|CT24931|FBan0008542 100% Plus / Plus *F bs04e09.y1 AI944715 Contig510 GH21762.5prime 99% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 99% Plus / Plus *F bs04e10.y1 AI944716 Contig544 none none *F bs04e11.y1 AI944717 Contig530 CG6262|FBan0006262|CT19610|FBan0006262 98% Plus / Plus *F bs04e12.y1 AI944718 Contig477 LP11903.5prime 82% Plus / Plus CG12334|FBan0012334|CT22897|FBan0012334 99% Plus / Plus *F bs04f01.y1 AI944719 singleton GM09767.5prime 99% Plus / Plus CG6382|FBan0006382|CT19890|FBan0006382 99% Plus / Plus *F bs04f03.y1 AI944720 Contig302 GH21955.5prime 99% Plus / Plus CG10652|FBan0010652|CT29824|FBan0010652 99% Plus / Plus *F bs04f04.y1 AI944721 Contig544 LD42288.5prime 99% Plus / Plus CG17161|FBan0017161|CT14720|FBan0017161 99% Plus / Plus *F bs04f05.y1 AI944722 Contig524 CG6943|FBan0006943|CT21523|FBan0006943 98% Plus / Plus *F bs04f06.y1 AI944723 Contig476 LP11595.5prime 98% Plus / Plus CG3752|FBan0003752|CT12541|FBan0003752 99% Plus / Plus *F bs04f07.y1 AI944724 Contig469 CG8732|FBan0008732|CT25221|FBan0008732 100% Plus / Minus *F bs04f09.y1 AI944725 Contig272 GH22856.5prime 100% Plus / Minus CG9485|FBan0009485|CT26790|FBan0009485 99% Plus / Minus *F bs04f10.y1 AI944726 singleton CK00068.3prime 97% Plus / Minus CG9485|FBan0009485|CT26790|FBan0009485 98% Plus / Minus *F bs04f11.y1 AI944727 singleton CG1980|FBan0001980|CT6205|FBan0001980 99% Plus / Plus *F bs04f12.y1 AI944728 Contig524 GH02751.3prime 83% Plus / Minus CG11023|FBan0011023|CT9573|FBan0011023 100% Plus / Plus *F bs04g01.y1 AI944729 singleton CG9997|FBan0009997|CT28179|FBan0009997 99% Plus / Plus *F bs04g02.y1 AI944730 Contig487 CG12470|FBan0012470|CT32706|FBan0012470 100% Plus / Plus *F bs04g03.y1 AI944731 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs04g04.y1 AI944732 Contig455 CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs04g05.y1 AI944733 Contig524 CG15179|FBan0015179|CT35094|FBan0015179 100% Plus / Plus *F bs04g06.y1 AI944734 Contig543 GH14070.5prime 97% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 97% Plus / Plus *F bs04g07.y1 AI944735 Contig273 CG6209|FBan0006209|CT19464|FBan0006209 95% Plus / Plus *F bs04g08.y1 AI944736 Contig519 GH24914.5prime 100% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs04g09.y1 AI944737 Contig271 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs04g10.y1 AI944738 Contig270 GH09028.5prime 99% Plus / Plus CG10977|FBan0010977|CT30763|FBan0010977 99% Plus / Plus *F bs04g11.y1 AI944739 Contig544 none none *F bs04g12.y1 AI944740 singleton GH01034.5prime 99% Plus / Plus CG5886|FBan0005886|CT18475|FBan0005886 99% Plus / Plus *F bs04h01.y1 AI944741 Contig269 GH13187.5prime 98% Plus / Plus CG15086|FBan0015086|CT34961|FBan0015086 98% Plus / Plus *F bs04h02.y1 pending Contig543 GH11908.5prime 98% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 98% Plus / Plus *F bs04h03.y1 AI944742 Contig268 GH25314.5prime 99% Plus / Plus CG8654|FBan0008654|CT25029|FBan0008654 99% Plus / Plus *F bs04h04.y1 AI944743 Contig267 GH13480.5prime 100% Plus / Plus CG4995|FBan0004995|CT16036|FBan0004995 100% Plus / Plus *F bs04h05.y1 AI944744 Contig529 LP04643.5prime 99% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs04h06.y1 AI944745 Contig467 GH04277.5prime 99% Plus / Plus CG6541|FBan0006541|CT20379|FBan0006541 99% Plus / Plus *F bs04h07.y1 AI944559 Contig527 CG16821|FBan0016821|CT35572|FBan0016821 99% Plus / Plus *F bs04h08.y1 AI944560 Contig342 SD10289.5prime 100% Plus / Plus CG5504|FBan0005504|CT17450|FBan0005504 99% Plus / Plus *F bs04h09.y1 AI944561 Contig542 GH26674.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs04h10.y1 AI944562 Contig544 CG4392|FBan0004392|CT14260|FBan0004392 98% Plus / Plus *F bs04h11.y1 AI944563 singleton LP10877.5prime 98% Plus / Plus CG1009|FBan0001009|CT1016|FBan0001009 98% Plus / Plus *F bs04h12.y1 AI944564 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs05a01.y1 AI944565 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 96% Plus / Plus *F bs05a02.y1 AI944566 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 97% Plus / Plus *F bs05a03.y1 AI944567 Contig498 GH25878.5prime 98% Plus / Plus CG18461|FBan0018461|CT38382|FBan0018461 99% Plus / Plus *F bs05a04.y1 AI944568 Contig499 GH19122.3prime 100% Plus / Minus CG18184|FBan0018184|CT41084|FBan0018184 100% Plus / Plus *F bs05a05.y1 AI944569 singleton GH02734.5prime 98% Plus / Plus none none *F bs05a06.y1 AI944746 Contig394 CG7059|FBan0007059|CT21831|FBan0007059 99% Plus / Plus *F bs05a07.y1 AI944747 Contig474 GH11908.5prime 97% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 97% Plus / Plus *F bs05a08.y1 AI944748 singleton CG3014|FBan0003014|CT10142|FBan0003014 98% Plus / Plus *F bs05a09.y1 AI944749 Contig466 GH20874.5prime 99% Plus / Plus CG2267|FBan0002267|CT7048|FBan0002267 99% Plus / Plus *F bs05a10.y1 pending singleton CG15484|FBan0015484|CT35585|FBan0015484 97% Plus / Plus *F bs05a11.y1 AI944750 Contig525 CG7848|FBan0007848|CT23768|FBan0007848 92% Plus / Plus *F bs05a12.y1 AI944751 Contig419 GH19789.5prime 94% Plus / Plus CG14740|FBan0014740|CT34534|FBan0014740 95% Plus / Plus *F bs05b01.y1 AI944752 Contig266 SD10439.5prime 99% Plus / Plus CG8979|FBan0008979|CT25810|FBan0008979 99% Plus / Plus *F bs05b02.y1 AI944753 singleton GH18753.5prime 100% Plus / Plus CG17654|FBan0017654|CT32526|FBan0017654 100% Plus / Plus *F bs05b03.y1 AI944754 singleton SD07039.5prime 94% Plus / Plus none none *F bs05b04.y1 AI944755 Contig166 CG10202|FBan0010202|CT28705|FBan0010202 100% Plus / Plus *F bs05b05.y1 AI944756 singleton none none *F bs05b06.y1 AI944757 Contig533 GH21951.5prime 100% Plus / Plus none none *F bs05b07.y1 AI944758 Contig540 GH08244.5prime 99% Plus / Plus CG17137|FBan0017137|CT38066|FBan0017137 99% Plus / Plus *F bs05b08.y1 AI944759 Contig532 GH16267.5prime 96% Plus / Plus CG17349|FBan0017349|CT32310|FBan0017349 96% Plus / Plus *F bs05b09.y1 AI944760 Contig430 none none *F bs05b10.y1 AI944761 Contig493 CG14926|FBan0014926|CT34753|FBan0014926 100% Plus / Plus *F bs05b11.y1 AI944762 singleton CG10869|FBan0010869|CT30429|FBan0010869 100% Plus / Plus *F bs05b12.y1 AI944763 Contig265 CG2964|FBan0002964|CT10035|FBan0002964 99% Plus / Plus *F bs05c01.y1 AI944764 singleton LD36932.5prime 100% Plus / Plus CG16917|FBan0016917|CT37528|FBan0016917 100% Plus / Plus *F bs05c02.y1 AI944765 Contig264 GH25176.5prime 100% Plus / Plus CG8526|FBan0008526|CT24903|FBan0008526 100% Plus / Plus *F bs05c04.y1 AI944766 Contig543 CG4750|FBan0004750|CT15217|FBan0004750 97% Plus / Plus *F bs05c05.y1 AI944767 Contig263 none none *F bs05c06.y1 AI944768 Contig387 GH13802.5prime 99% Plus / Plus CG1314|FBan0001314|CT2886|FBan0001314 100% Plus / Plus *F bs05c07.y1 AI944769 Contig262 LD14154.5prime 97% Plus / Plus CG18638|FBan0018638|CT42589|FBan0018638 99% Plus / Plus *F bs05c08.y1 AI944770 Contig522 LP09246.5prime 99% Plus / Plus none none *F bs05c09.y1 AI944771 singleton CG2574|FBan0002574|CT8709|FBan0002574 100% Plus / Plus *F bs05c10.y1 AI944772 singleton none none *F bs05c11.y1 AI944773 Contig544 GH19274.5prime 98% Plus / Plus CG9130|FBan0009130|CT10095|FBan0009130 99% Plus / Plus *F bs05c12.y1 AI944774 Contig544 GH22881.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs05d01.y1 AI944775 Contig544 GH10815.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs05d02.y1 AI944776 Contig434 CG7164|FBan0007164|CT22119|FBan0007164 100% Plus / Plus *F bs05d03.y1 AI944777 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs05d05.y1 AI944778 Contig499 CG8709|FBan0008709|CT9407|FBan0008709 100% Plus / Plus *F bs05d06.y1 AI944779 singleton CG1988|FBan0001988|CT6344|FBan0001988 100% Plus / Plus *F bs05d07.y1 AI944780 Contig542 GH21096.5prime 96% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs05d08.y1 AI944781 singleton GH15018.5prime 99% Plus / Plus CG17737|FBan0017737|CT2632|FBan0017737 100% Plus / Plus *F bs05d09.y1 AI944782 Contig261 CK02305.5prime 99% Plus / Plus CG17949|FBan0017949|CT39990|FBan0017949 97% Plus / Plus *F bs05d10.y1 AI944783 Contig383 LD10355.5prime 100% Plus / Plus CG2512|FBan0002512|CT8333|FBan0002512 100% Plus / Plus *F bs05d11.y1 AI944784 Contig412 GH14048.5prime 100% Plus / Plus CG7131|FBan0007131|CT22049|FBan0007131 100% Plus / Plus *F bs05d12.y1 AI944785 singleton LP10336.5prime 97% Plus / Plus CG6590|FBan0006590|CT20492|FBan0006590 97% Plus / Plus *F bs05e01.y1 AI944786 Contig540 none none *F bs05e02.y1 AI944787 Contig260 CG6815|FBan0006815|CT19832|FBan0006815 99% Plus / Plus *F bs05e03.y1 AI944788 singleton CG5348|FBan0005348|CT16901|FBan0005348 99% Plus / Plus *F bs05e04.y1 AI944789 Contig539 GH17440.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs05e05.y1 AI944790 singleton CG18341|FBan0018341|CT41603|FBan0018341 100% Plus / Plus *F bs05e06.y1 AI944791 singleton GM09619.5prime 100% Plus / Plus CG12233|FBan0012233|CT13154|FBan0012233 100% Plus / Plus *F bs05e07.y1 AI944792 singleton LD33633.5prime 98% Plus / Plus none none *F bs05e08.y1 AI944793 Contig386 HL01913.5prime 97% Plus / Plus CG1225|FBan0001225|CT2308|FBan0001225 100% Plus / Plus *F bs05e09.y1 AI944794 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs05e10.y1 AI944795 singleton CG10669|FBan0010669|CT29884|FBan0010669 99% Plus / Plus *F bs05e11.y1 AI944796 Contig259 LD21467.5prime 100% Plus / Plus CG9329|FBan0009329|CT3799|FBan0009329 99% Plus / Plus *F bs05e12.y1 AI944797 Contig490 none none *F bs05f01.y1 AI944798 Contig526 LP08710.5prime 99% Plus / Plus CG9881|FBan0009881|CT9620|FBan0009881 99% Plus / Plus *F bs05f02.y1 AI944799 Contig413 CG7750|FBan0007750|CT23586|FBan0007750 100% Plus / Plus *F bs05f03.y1 AI944800 Contig258 CG9274|FBan0009274|CT26429|FBan0009274 100% Plus / Plus *F bs05f04.y1 AI944801 Contig256 CG6036|FBan0006036|CT18937|FBan0006036 99% Plus / Plus *F bs05f05.y1 AI944802 Contig521 GH27033.5prime 100% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs05f06.y1 AI944803 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs05f07.y1 AI944804 singleton LD45906.5prime 100% Plus / Plus CG3073|FBan0003073|CT9957|FBan0003073 100% Plus / Plus *F bs05f08.y1 AI944805 Contig384 CG3345|FBan0003345|CT36339|FBan0003345 99% Plus / Plus *F bs05f09.y1 AI944806 Contig472 CG9129|FBan0009129|CT8135|FBan0009129 99% Plus / Plus *F bs05f10.y1 AI944807 singleton none none *F bs05f11.y1 AI944808 Contig464 CG14297|FBan0014297|CT33927|FBan0014297 99% Plus / Plus *F bs05f12.y1 AI944809 Contig255 CG11983|FBan0011983|CT32135|FBan0011983 99% Plus / Plus *F bs05g01.y1 AI944810 Contig383 LD28345.5prime 99% Plus / Plus CG2512|FBan0002512|CT8333|FBan0002512 99% Plus / Plus *F bs05g02.y1 AI944811 singleton LP07130.5prime 99% Plus / Plus CG4578|FBan0004578|CT14822|FBan0004578 100% Plus / Plus *F bs05g03.y1 AI944812 Contig534 none none *F bs05g04.y1 AI944813 singleton none none *F bs05g05.y1 AI944814 Contig544 GH22513.5prime 96% Plus / Plus none none *F bs05g06.y1 AI944815 singleton GH03396.3prime 99% Plus / Plus CG1287|FBan0001287|CT2727|FBan0001287 99% Plus / Minus *F bs05g07.y1 AI944816 Contig254 GH25896.5prime 100% Plus / Plus CG5903|FBan0005903|CT18297|FBan0005903 100% Plus / Plus *F bs05g08.y1 AI944817 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs05g09.y1 AI944818 Contig296 CG13394|FBan0013394|CT32740|FBan0013394 97% Plus / Plus *F bs05g10.y1 AI944819 singleton none none *F bs05g11.y1 AI944820 singleton HL02756.5prime 98% Plus / Plus CG8494|FBan0008494|CT24839|FBan0008494 99% Plus / Plus *F bs05g12.y1 AI944821 Contig470 CG17450|FBan0017450|CT34184|FBan0017450 99% Plus / Plus *F bs05h01.y1 AI944822 Contig499 GH20857.3prime 98% Plus / Minus CG6664|FBan0006664|CT20702|FBan0006664 99% Plus / Plus *F bs05h02.y1 AI944823 Contig450 GH23475.5prime 100% Plus / Plus none none *F bs05h03.y1 AI944824 singleton CG18028|FBan0018028|CT40348|FBan0018028 99% Plus / Plus *F bs05h04.y1 AI944825 Contig382 CG8750|FBan0008750|CT25256|FBan0008750 100% Plus / Plus *F bs05h05.y1 pending singleton CG7755|FBan0007755|CT23592|FBan0007755 99% Plus / Plus *F bs05h06.y1 AI944826 Contig543 GH09553.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs05h07.y1 AI944827 Contig429 CG6380|FBan0006380|CT19908|FBan0006380 99% Plus / Plus *F bs05h08.y1 AI944828 singleton none none *F bs05h09.y1 AI944829 singleton CG12593|FBan0012593|CT34497|FBan0012593 100% Plus / Minus *F bs05h10.y1 AI944830 singleton GH14578.5prime 100% Plus / Plus CG3323|FBan0003323|CT11173|FBan0003323 100% Plus / Plus *F bs05h11.y1 AI944831 Contig530 GH13953.5prime 100% Plus / Minus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Minus *F bs05h12.y1 AI944832 Contig254 GH25896.5prime 100% Plus / Plus CG5903|FBan0005903|CT18297|FBan0005903 100% Plus / Plus *F bs06a02.y1 AI944833 Contig381 HL01528.5prime 97% Plus / Plus CG5735|FBan0005735|CT18021|FBan0005735 98% Plus / Plus *F bs06a03.y1 AI944834 singleton CG17003|FBan0017003|CT35311|FBan0017003 100% Plus / Plus *F bs06a04.y1 AI944835 Contig253 CG15878|FBan0015878|CT33288|FBan0015878 100% Plus / Plus *F bs06a05.y1 AI944836 singleton LP10165.5prime 98% Plus / Plus CG18640|FBan0018640|CT42651|FBan0018640 98% Plus / Plus *F bs06a06.y1 AI944837 singleton CG15481|FBan0015481|CT35575|FBan0015481 100% Plus / Plus *F bs06a07.y1 pending Contig385 none none *F bs06a08.y1 AI944838 singleton none none *F bs06a10.y1 AI944839 Contig511 GH22851.5prime 98% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs06a11.y1 AI944840 Contig533 GH21951.5prime 96% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 97% Plus / Plus *F bs06a12.y1 AI944841 Contig528 CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Minus *F bs06b01.y1 AI944842 singleton CG14739|FBan0014739|CT34533|FBan0014739 99% Plus / Plus *F bs06b02.y1 AI944843 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs06b03.y1 AI944844 singleton none none *F bs06b04.y1 AI944845 singleton CG8260|FBan0008260|CT24475|FBan0008260 100% Plus / Plus *F bs06b05.y1 AI944846 Contig431 none none *F bs06b06.y1 AI944847 Contig252 CG9235|FBan0009235|CT26382|FBan0009235 100% Plus / Plus *F bs06b07.y1 AI944848 singleton LD27466.5prime 100% Plus / Plus CG13298|FBan0013298|CT32586|FBan0013298 100% Plus / Plus *F bs06b08.y1 AI944849 Contig432 LD42819.5prime 100% Plus / Plus CG8938|FBan0008938|CT25660|FBan0008938 100% Plus / Plus *F bs06b09.y1 AI944850 singleton GH12925.3prime 98% Plus / Minus CG2291|FBan0002291|CT7606|FBan0002291 97% Plus / Plus *F bs06b10.y1 AI944851 singleton LD06702.5prime 99% Plus / Plus CG1823|FBan0001823|CT5418|FBan0001823 99% Plus / Plus *F bs06b11.y1 AI944852 singleton none none *F bs06b12.y1 AI944853 Contig251 CG5435|FBan0005435|CT17244|FBan0005435 99% Plus / Plus *F bs06c01.y1 AI944854 Contig405 CG10164|FBan0010164|CT28591|FBan0010164 100% Plus / Plus *F bs06c02.y1 pending Contig433 GH13115.5prime 100% Plus / Plus CG4265|FBan0004265|CT10917|FBan0004265 100% Plus / Plus *F bs06c03.y1 AI944855 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 100% Plus / Plus *F bs06c04.y1 AI944856 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs06c05.y1 AI944857 Contig333 LP09083.5prime 88% Plus / Plus CG9476|FBan0009476|CT26846|FBan0009476 100% Plus / Plus *F bs06c06.y1 pending singleton LD23231.5prime 99% Plus / Plus CG6720|FBan0006720|CT20873|FBan0006720 100% Plus / Plus *F bs06c07.y1 AI944858 Contig249 CG5432|FBan0005432|CT17240|FBan0005432 95% Plus / Plus *F bs06c08.y1 AI944859 singleton CG4917|FBan0004917|CT15800|FBan0004917 99% Plus / Plus *F bs06c09.y1 AI944860 Contig414 CG12853|FBan0012853|CT31992|FBan0012853 100% Plus / Plus *F bs06c10.y1 AI944861 Contig248 CG5265|FBan0005265|CT16817|FBan0005265 97% Plus / Plus *F bs06c11.y1 pending singleton none none *F bs06c12.y1 AI944862 singleton GH11019.5prime 100% Plus / Plus CG9159|FBan0009159|CT26208|FBan0009159 100% Plus / Plus *F bs06d01.y1 AI944863 singleton HL02330.5prime 100% Plus / Plus CG7945|FBan0007945|CT37755|FBan0007945 100% Plus / Plus *F bs06d03.y1 AI944864 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs06d04.y1 AI944865 singleton CG9801|FBan0009801|CT36839|FBan0009801 100% Plus / Plus *F bs06d05.y1 AI944866 singleton CG12464|FBan0012464|CT32655|FBan0012464 98% Plus / Plus *F bs06d06.y1 AI944867 singleton CG15196|FBan0015196|CT35118|FBan0015196 100% Plus / Plus *F bs06d07.y1 AI944868 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs06d08.y1 AI944869 Contig531 GH04958.5prime 96% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 96% Plus / Plus *F bs06d09.y1 AI944870 Contig247 GH23081.5prime 98% Plus / Minus CG17956|FBan0017956|CT40004|FBan0017956 97% Plus / Minus *F bs06d10.y1 AI944871 Contig346 LP05376.5prime 97% Plus / Minus CG8448|FBan0008448|CT37123|FBan0008448 99% Plus / Plus *F bs06d11.y1 AI944872 Contig544 GH25863.5prime 99% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 99% Plus / Plus *F bs06d12.y1 AI944873 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs06e01.y1 AI944874 Contig496 LD36220.5prime 97% Plus / Plus CG7892|FBan0007892|CT23293|FBan0007892 100% Plus / Plus *F bs06e02.y1 AI944875 Contig489 LP11879.5prime 100% Plus / Plus CG5045|FBan0005045|CT16189|FBan0005045 100% Plus / Plus *F bs06e03.y1 AI944876 singleton GH04159.3prime 98% Plus / Minus CG16760|FBan0016760|CT8050|FBan0016760 98% Plus / Plus *F bs06e04.y1 AI944877 Contig530 GH13953.5prime 99% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 98% Plus / Plus *F bs06e05.y1 AI944878 singleton CG3610|FBan0003610|CT12129|FBan0003610 99% Plus / Plus *F bs06e06.y1 AI944879 singleton GM04207.5prime 98% Plus / Plus CG10340|FBan0010340|CT29032|FBan0010340 100% Plus / Plus *F bs06e07.y1 AI944880 Contig246 GH20396.5prime 98% Plus / Plus CG3809|FBan0003809|CT12693|FBan0003809 98% Plus / Plus *F bs06e08.y1 AI944881 singleton CG4008|FBan0004008|CT13300|FBan0004008 100% Plus / Plus *F bs06e09.y1 AI944882 Contig401 LP07372.5prime 100% Plus / Minus CG3213|FBan0003213|CT10803|FBan0003213 100% Plus / Plus *F bs06e10.y1 AI944883 Contig469 CG8732|FBan0008732|CT25221|FBan0008732 100% Plus / Minus *F bs06e11.y1 AI944884 singleton CG13241|FBan0013241|CT32491|FBan0013241 99% Plus / Plus *F bs06e12.y1 AI944885 Contig245 CK00367.3prime 95% Plus / Minus CG15111|FBan0015111|CT34989|FBan0015111 100% Plus / Plus *F bs06f01.y1 AI944886 singleton LD41266.5prime 100% Plus / Plus CG1759|FBan0001759|CT5072|FBan0001759 100% Plus / Plus *F bs06f02.y1 AI944887 Contig380 CG13778|FBan0013778|CT33266|FBan0013778 98% Plus / Minus *F bs06f03.y1 AI944888 Contig508 CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs06f04.y1 AI944889 Contig407 CG3399|FBan0003399|CT11427|FBan0003399 99% Plus / Plus *F bs06f05.y1 AI944890 Contig439 GH07654.5prime 100% Plus / Plus CG8994|FBan0008994|CT25812|FBan0008994 100% Plus / Plus *F bs06f06.y1 pending Contig244 CG17083|FBan0017083|CT37958|FBan0017083 99% Plus / Plus *F bs06f07.y1 AI944891 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs06f08.y1 AI944892 singleton none none *F bs06f10.y1 AI944893 singleton GH25043.5prime 99% Plus / Plus CG7188|FBan0007188|CT22197|FBan0007188 99% Plus / Plus *F bs06f11.y1 AI944894 Contig533 GH06096.5prime 100% Plus / Plus none none *F bs06f12.y1 AI944895 singleton CG17110|FBan0017110|CT38008|FBan0017110 99% Plus / Plus *F bs06g01.y1 AI944896 Contig379 LD23918.3prime 98% Plus / Minus CG8073|FBan0008073|CT8072|FBan0008073 100% Plus / Plus *F bs06g02.y1 AI944897 Contig244 CG17083|FBan0017083|CT37958|FBan0017083 100% Plus / Plus *F bs06g03.y1 pending singleton none none *F bs06g04.y1 pending Contig531 GH04958.5prime 98% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 97% Plus / Plus *F bs06g05.y1 AI944898 singleton GH16447.5prime 98% Plus / Plus none none *F bs06g06.y1 AI944899 Contig350 LD26230.5prime 99% Plus / Plus CG5405|FBan0005405|CT17132|FBan0005405 99% Plus / Plus *F bs06g07.y1 AI944900 singleton CG6471|FBan0006471|CT20183|FBan0006471 100% Plus / Plus *F bs06g08.y1 AI944901 Contig525 GM14265.5prime 100% Plus / Plus CG8983|FBan0008983|CT25820|FBan0008983 99% Plus / Plus *F bs06g09.y1 AI944902 singleton none none *F bs06g10.y1 AI944903 Contig510 GH21762.5prime 99% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 99% Plus / Plus *F bs06g11.y1 AI944904 singleton GH18692.5prime 99% Plus / Plus CG4095|FBan0004095|CT13576|FBan0004095 99% Plus / Plus *F bs06g12.y1 AI944905 singleton CG12677|FBan0012677|CT35495|FBan0012677 100% Plus / Plus *F bs06h01.y1 AI944906 Contig519 GH05452.5prime 99% Plus / Minus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Minus *F bs06h02.y1 pending Contig252 CG9235|FBan0009235|CT26382|FBan0009235 97% Plus / Plus *F bs06h03.y1 AI944907 singleton LP07046.5prime 100% Plus / Plus none none *F bs06h04.y1 AI944908 singleton LP02452.5prime 100% Plus / Plus none none *F bs06h05.y1 AI944909 Contig243 none none *F bs06h06.y1 AI944910 Contig423 LD15713.5prime 99% Plus / Plus CG1383|FBan0001383|CT3168|FBan0001383 100% Plus / Plus *F bs06h07.y1 AI944911 singleton GH25049.5prime 100% Plus / Plus CG8189|FBan0008189|CT24393|FBan0008189 100% Plus / Plus *F bs06h08.y1 AI944912 Contig242 LP10502.5prime 100% Plus / Plus CG1548|FBan0001548|CT3996|FBan0001548 100% Plus / Plus *F bs06h09.y1 AI944913 singleton none none *F bs06h10.y1 AI944914 singleton CG13564|FBan0013564|CT32944|FBan0013564 99% Plus / Plus *F bs06h11.y1 AI944915 singleton none none *F bs06h12.y1 AI944916 Contig386 HL01913.5prime 97% Plus / Plus CG1225|FBan0001225|CT2308|FBan0001225 99% Plus / Plus *F bs07a01.y1 AI944917 Contig544 none none *F bs07a02.y1 AI944918 Contig393 CG18427|FBan0018427|CT30112|FBan0018427 100% Plus / Plus *F bs07a03.y1 AI944919 Contig241 LP05266.5prime 99% Plus / Plus CG14717|FBan0014717|CT34508|FBan0014717 99% Plus / Plus *F bs07a04.y1 AI944920 Contig240 CG15260|FBan0015260|CT35204|FBan0015260 99% Plus / Plus *F bs07a05.y1 AI944921 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs07a06.y1 AI944922 Contig503 GH11521.5prime 95% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 96% Plus / Plus *F bs07a07.y1 AI944923 singleton none none *F bs07a08.y1 AI944924 Contig223 CG9966|FBan0009966|CT28103|FBan0009966 99% Plus / Plus *F bs07a09.y1 AI944925 singleton LD45374.5prime 100% Plus / Plus CG3183|FBan0003183|CT10671|FBan0003183 99% Plus / Plus *F bs07a10.y1 AI944926 singleton CG11373|FBan0011373|CT31752|FBan0011373 99% Plus / Plus *F bs07a11.y1 AI944927 Contig378 CG1137|FBan0001137|CT1904|FBan0001137 99% Plus / Plus *F bs07a12.y1 AI944928 Contig419 GH19789.5prime 95% Plus / Plus CG14740|FBan0014740|CT34534|FBan0014740 96% Plus / Plus *F bs07b01.y1 AI944929 singleton none none *F bs07b02.y1 AI944930 Contig441 GH26265.5prime 100% Plus / Plus none none *F bs07b03.y1 AI944931 Contig542 GH28631.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs07b04.y1 AI944932 Contig499 GH19122.3prime 100% Plus / Minus CG18184|FBan0018184|CT41084|FBan0018184 100% Plus / Plus *F bs07b05.y1 AI944933 Contig377 LP04712.5prime 99% Plus / Plus none none *F bs07b06.y1 AI944934 singleton none none *F bs07b07.y1 AI944935 Contig504 CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs07b08.y1 AI944936 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs07b09.y1 AI944937 Contig239 GH09348.5prime 99% Plus / Plus CG12790|FBan0012790|CT37283|FBan0012790 100% Plus / Plus *F bs07b10.y1 AI944938 Contig530 GH13953.5prime 99% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs07b11.y1 AI944939 Contig374 none none *F bs07b12.y1 AI944940 Contig405 CG10164|FBan0010164|CT28591|FBan0010164 100% Plus / Plus *F bs07c01.y1 AI944941 Contig447 GH12486.5prime 97% Plus / Plus CG4375|FBan0004375|CT14270|FBan0004375 97% Plus / Plus *F bs07c02.y1 AI944942 Contig455 CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs07c03.y1 AI944943 Contig376 LP04452.5prime 99% Plus / Plus CG9920|FBan0009920|CT27932|FBan0009920 99% Plus / Plus *F bs07c04.y1 AI944944 singleton GH15116.5prime 99% Plus / Plus CG12013|FBan0012013|CT1649|FBan0012013 100% Plus / Plus *F bs07c05.y1 AI944945 Contig357 SD02767.5prime 100% Plus / Plus CG13176|FBan0013176|CT32417|FBan0013176 100% Plus / Plus *F bs07c06.y1 AI944946 Contig535 GH21036.5prime 100% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 100% Plus / Plus *F bs07c07.y1 AI944947 Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs07c08.y1 AI944948 Contig543 none none *F bs07c09.y1 AI944949 Contig368 CG3494|FBan0003494|CT11751|FBan0003494 98% Plus / Plus *F bs07c10.y1 AI944950 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs07c11.y1 AI944951 singleton CG12949|FBan0012949|CT32100|FBan0012949 100% Plus / Minus *F bs07c12.y1 AI944952 Contig238 CK01731.3prime 95% Plus / Minus CG8368|FBan0008368|CT24647|FBan0008368 99% Plus / Plus *F bs07d01.y1 AI944953 Contig540 GH25305.5prime 99% Plus / Plus CG9602|FBan0009602|CT27154|FBan0009602 99% Plus / Plus *F bs07d02.y1 AI944954 singleton LP04225.5prime 99% Plus / Plus CG3875|FBan0003875|CT12835|FBan0003875 99% Plus / Plus *F bs07d03.y1 AI944955 Contig443 CG10734|FBan0010734|CT30089|FBan0010734 97% Plus / Plus *F bs07d04.y1 AI944956 singleton CG4961|FBan0004961|CT15711|FBan0004961 100% Plus / Plus *F bs07d05.y1 AI944957 singleton SD09444.5prime 100% Plus / Minus CG10813|FBan0010813|CT30310|FBan0010813 100% Plus / Minus *F bs07d06.y1 AI944958 singleton CG8923|FBan0008923|CT25630|FBan0008923 99% Plus / Plus *F bs07d07.y1 AI944959 Contig482 CG18449|FBan0018449|CT42026|FBan0018449 99% Plus / Plus *F bs07d08.y1 AI944960 Contig431 none none *F bs07d09.y1 AI944961 singleton GM10228.5prime 100% Plus / Plus CG7414|FBan0007414|CT22817|FBan0007414 100% Plus / Plus *F bs07d10.y1 AI944962 Contig406 GH05530.5prime 99% Plus / Plus CG17567|FBan0017567|CT35086|FBan0017567 99% Plus / Plus *F bs07d11.y1 AI944963 Contig544 LP04942.5prime 100% Plus / Plus CG3702|FBan0003702|CT12347|FBan0003702 99% Plus / Plus *F bs07e01.y1 AI944964 Contig544 CG12357|FBan0012357|CT23846|FBan0012357 100% Plus / Plus *F bs07e02.y1 AI944965 Contig544 GH20547.5prime 100% Plus / Plus none none *F bs07e03.y1 AI944966 singleton CG13520|FBan0013520|CT32891|FBan0013520 99% Plus / Plus *F bs07e05.y1 AI944967 singleton CG9655|FBan0009655|CT27290|FBan0009655 100% Plus / Plus *F bs07e06.y1 AI944968 Contig415 GH05991.5prime 98% Plus / Plus CG9106|FBan0009106|CT26128|FBan0009106 98% Plus / Plus *F bs07e07.y1 AI944969 singleton CG4272|FBan0004272|CT9361|FBan0004272 100% Plus / Plus *F bs07e08.y1 AI944970 singleton none none *F bs07e09.y1 AI944971 Contig237 CG9389|FBan0009389|CT26645|FBan0009389 100% Plus / Plus *F bs07e10.y1 AI944972 Contig487 none none *F bs07e11.y1 AI944973 Contig534 none none *F bs07e12.y1 AI944974 singleton CG4323|FBan0004323|CT14141|FBan0004323 100% Plus / Plus *F bs07f01.y1 AI944975 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs07f02.y1 AI944976 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs07f03.y1 AI944977 Contig502 GH01042.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs07f04.y1 AI944978 Contig318 GH14384.5prime 99% Plus / Plus CG9131|FBan0009131|CT26166|FBan0009131 99% Plus / Plus *F bs07f06.y1 AI944979 Contig235 CG7235|FBan0007235|CT22309|FBan0007235 99% Plus / Minus *F bs07f07.y1 AI944980 Contig542 GH21803.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs07f08.y1 AI944981 singleton none none *F bs07f09.y1 AI944982 singleton GH24822.5prime 99% Plus / Plus CG10731|FBan0010731|CT30079|FBan0010731 99% Plus / Plus *F bs07f10.y1 AI944983 Contig426 CG10551|FBan0010551|CT29604|FBan0010551 99% Plus / Plus *F bs07f11.y1 AI944984 singleton LP05029.5prime 100% Plus / Plus CG1407|FBan0001407|CT3248|FBan0001407 100% Plus / Plus *F bs07f12.y1 AI944985 singleton LD41801.5prime 99% Plus / Plus CG5915|FBan0005915|CT18555|FBan0005915 99% Plus / Plus *F bs07g01.y1 AI944986 Contig289 LP10051.5prime 100% Plus / Plus CG6692|FBan0006692|CT20780|FBan0006692 100% Plus / Plus *F bs07g02.y1 AI944987 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs07g04.y1 AI944988 Contig435 LP05191.5prime 100% Plus / Plus CG12562|FBan0012562|CT34305|FBan0012562 100% Plus / Plus *F bs07g05.y1 AI944989 Contig234 none none *F bs07g06.y1 AI944990 singleton LD20550.5prime 97% Plus / Plus CG12211|FBan0012211|CT11197|FBan0012211 99% Plus / Plus *F bs07g07.y1 AI944991 Contig521 GH07596.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs07g08.y1 AI944992 Contig513 LP02115.5prime 100% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs07g09.y1 AI944993 singleton HL06140.5prime 96% Plus / Plus CG12338|FBan0012338|CT23223|FBan0012338 100% Plus / Plus *F bs07g10.y1 AI944994 singleton LD13774.3prime 100% Plus / Plus CG5303|FBan0005303|CT16641|FBan0005303 100% Plus / Minus *F bs07g11.y1 AI944995 Contig232 CK01340.5prime 92% Plus / Minus CG4686|FBan0004686|CT15101|FBan0004686 100% Plus / Plus *F bs07g12.y1 AI944996 Contig381 GH16751.5prime 98% Plus / Plus CG5735|FBan0005735|CT18021|FBan0005735 98% Plus / Plus *F bs07h01.y1 AI944997 Contig231 CG18063|FBan0018063|CT40483|FBan0018063 98% Plus / Plus *F bs07h02.y1 AI944998 singleton CG15287|FBan0015287|CT35235|FBan0015287 98% Plus / Plus *F bs07h03.y1 AI944999 Contig234 none none *F bs07h04.y1 AI945000 Contig458 GH04159.5prime 99% Plus / Plus CG16758|FBan0016758|CT8044|FBan0016758 99% Plus / Plus *F bs07h05.y1 AI945001 singleton GM03811.5prime 97% Plus / Plus none none *F bs07h06.y1 AI945002 Contig544 GH22513.5prime 100% Plus / Plus none none *F bs07h07.y1 AI945003 Contig374 none none *F bs07h08.y1 AI945004 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs07h09.y1 AI945005 Contig373 LD06040.5prime 100% Plus / Plus CG4390|FBan0004390|CT14318|FBan0004390 100% Plus / Plus *F bs07h10.y1 AI945006 singleton GM04707.5prime 99% Plus / Plus CG10465|FBan0010465|CT29380|FBan0010465 99% Plus / Plus *F bs07h11.y1 AI945007 singleton CG18115|FBan0018115|CT40781|FBan0018115 98% Plus / Plus *F bs07h12.y1 AI945008 singleton CG16988|FBan0016988|CT37703|FBan0016988 100% Plus / Plus *F bs08a01.y1 AI945009 singleton SD02428.5prime 98% Plus / Plus CG8980|FBan0008980|CT25804|FBan0008980 100% Plus / Plus *F bs08a02.y1 AI945010 singleton GH07743.5prime 99% Plus / Plus CG3092|FBan0003092|CT10386|FBan0003092 99% Plus / Plus *F bs08a03.y1 AI945011 Contig541 GH20441.5prime 100% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 100% Plus / Plus *F bs08a04.y1 AI945012 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs08a05.y1 AI945013 Contig513 LP02115.5prime 98% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs08a06.y1 AI945014 Contig372 GH13257.5prime 98% Plus / Plus CG17010|FBan0017010|CT37753|FBan0017010 99% Plus / Plus *F bs08a07.y1 AI945015 Contig438 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs08a08.y1 AI945016 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 97% Plus / Plus *F bs08a09.y1 AI945017 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs08a10.y1 AI945018 Contig301 GH02452.5prime 99% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 100% Plus / Plus *F bs08a11.y1 AI945019 Contig543 GH18035.5prime 99% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 99% Plus / Plus *F bs08a12.y1 AI945020 singleton GH03711.5prime 100% Plus / Plus CG11876|FBan0011876|CT37034|FBan0011876 100% Plus / Plus *F bs08b01.y1 AI945021 Contig230 CG6209|FBan0006209|CT19464|FBan0006209 100% Plus / Plus *F bs08b02.y1 AI945022 singleton CG11005|FBan0011005|CT8955|FBan0011005 100% Plus / Plus *F bs08b03.y1 AI945023 Contig229 LD45146.5prime 99% Plus / Plus CG7007|FBan0007007|CT21672|FBan0007007 99% Plus / Plus *F bs08b04.y1 AI945024 singleton CG15889|FBan0015889|CT34021|FBan0015889 99% Plus / Plus *F bs08b05.y1 AI945025 Contig505 GH08240.5prime 100% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 100% Plus / Plus *F bs08b06.y1 AI945026 singleton LD12818.3prime 100% Plus / Minus CG15513|FBan0015513|CT35626|FBan0015513 99% Plus / Plus *F bs08b07.y1 AI945027 Contig255 CG11983|FBan0011983|CT32135|FBan0011983 100% Plus / Plus *F bs08b08.y1 AI945028 Contig499 GH19122.3prime 99% Plus / Minus CG18184|FBan0018184|CT41084|FBan0018184 99% Plus / Plus *F bs08b09.y1 AI945029 Contig532 GH16267.5prime 96% Plus / Plus CG17349|FBan0017349|CT32310|FBan0017349 96% Plus / Plus *F bs08b10.y1 AI945030 Contig529 CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs08b11.y1 AI945031 singleton LD10395.5prime 97% Plus / Plus CG4898|FBan0004898|CT15051|FBan0004898 97% Plus / Plus *F bs08b12.y1 AI945032 Contig498 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT38382|FBan0018461 98% Plus / Plus *F bs08c01.y1 AI945033 singleton GM04070.5prime 98% Plus / Plus CG5174|FBan0005174|CT39031|FBan0005174 98% Plus / Plus *F bs08c02.y1 AI945034 Contig526 GH11587.5prime 99% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs08c03.y1 AI945035 singleton CG17301|FBan0017301|CT38284|FBan0017301 100% Plus / Minus *F bs08c04.y1 AI945036 singleton none none *F bs08c05.y1 AI945037 singleton CG13871|FBan0013871|CT33396|FBan0013871 99% Plus / Plus *F bs08c06.y1 AI945038 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs08c07.y1 AI945039 Contig542 GH28631.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs08c08.y1 AI945040 Contig399 GH07182.5prime 100% Plus / Plus CG5217|FBan0005217|CT16693|FBan0005217 100% Plus / Plus *F bs08c09.y1 AI945041 singleton GM04150.5prime 99% Plus / Plus CG6851|FBan0006851|CT21221|FBan0006851 100% Plus / Plus *F bs08c10.y1 AI945042 Contig533 GH09435.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs08c11.y1 AI945043 singleton none none *F bs08c12.y1 AI945044 singleton GH16473.5prime 99% Plus / Plus CG2209|FBan0002209|CT7336|FBan0002209 100% Plus / Plus *F bs08d01.y1 AI945045 Contig526 CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs08d02.y1 AI945046 singleton CG15133|FBan0015133|CT35028|FBan0015133 97% Plus / Plus *F bs08d03.y1 AI945047 Contig515 LD41653.5prime 100% Plus / Plus none none *F bs08d04.y1 AI945048 singleton none none *F bs08d05.y1 AI945049 Contig267 GH13480.5prime 99% Plus / Plus CG4995|FBan0004995|CT16036|FBan0004995 99% Plus / Plus *F bs08d06.y1 AI945050 Contig283 GH25549.5prime 97% Plus / Plus CG5261|FBan0005261|CT16733|FBan0005261 97% Plus / Plus *F bs08d07.y1 AI945051 singleton LD38188.5prime 98% Plus / Minus CG5216|FBan0005216|CT16687|FBan0005216 98% Plus / Minus *F bs08d08.y1 AI945052 Contig522 LP09246.5prime 99% Plus / Plus none none *F bs08d09.y1 AI945053 Contig400 GH25946.5prime 99% Plus / Plus CG14101|FBan0014101|CT33692|FBan0014101 99% Plus / Plus *F bs08d10.y1 AI945054 Contig448 CG18128|FBan0018128|CT40818|FBan0018128 99% Plus / Plus *F bs08d11.y1 AI945055 singleton CG6501|FBan0006501|CT20247|FBan0006501 100% Plus / Plus *F bs08e02.y1 AI945056 Contig228 SD05065.5prime 100% Plus / Plus CG8531|FBan0008531|CT24915|FBan0008531 100% Plus / Plus *F bs08e03.y1 AI945057 singleton none none *F bs08e04.y1 AI945058 singleton GH02390.5prime 98% Plus / Minus CG7920|FBan0007920|CT6599|FBan0007920 98% Plus / Minus *F bs08e05.y1 AI945059 singleton CG6235|FBan0006235|CT36963|FBan0006235 99% Plus / Plus *F bs08e06.y1 AI945060 Contig382 CG8750|FBan0008750|CT25256|FBan0008750 99% Plus / Plus *F bs08e07.y1 AI945061 singleton GH18827.5prime 99% Plus / Plus CG8893|FBan0008893|CT25538|FBan0008893 99% Plus / Plus *F bs08e08.y1 AI945062 singleton CG9692|FBan0009692|CT27398|FBan0009692 100% Plus / Plus *F bs08e09.y1 AI945063 Contig227 GH10330.5prime 99% Plus / Plus CG3494|FBan0003494|CT11751|FBan0003494 98% Plus / Plus *F bs08e10.y1 AI945064 singleton SD09093.5prime 100% Plus / Plus CG12019|FBan0012019|CT1735|FBan0012019 99% Plus / Plus *F bs08e11.y1 AI945065 Contig426 CK00552.5prime 98% Plus / Minus CG12908|FBan0012908|CT32053|FBan0012908 100% Plus / Minus *F bs08e12.y1 AI945066 singleton CG4676|FBan0004676|CT15093|FBan0004676 99% Plus / Plus *F bs08f02.y1 AI945067 Contig428 LP09525.5prime 100% Plus / Plus CG15219|FBan0015219|CT35155|FBan0015219 100% Plus / Plus *F bs08f03.y1 pending singleton LD47479.5prime 95% Plus / Plus CG10419|FBan0010419|CT29254|FBan0010419 95% Plus / Plus *F bs08f04.y1 AI945068 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs08f05.y1 AI945069 Contig449 CG9218|FBan0009218|CT26312|FBan0009218 100% Plus / Plus *F bs08f06.y1 AI945070 singleton GH27967.5prime 99% Plus / Plus CG8000|FBan0008000|CT24038|FBan0008000 99% Plus / Plus *F bs08f07.y1 AI945071 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs08f08.y1 AI945072 Contig371 CG14305|FBan0014305|CT33935|FBan0014305 99% Plus / Plus *F bs08f09.y1 AI945073 Contig370 GM02532.5prime 98% Plus / Plus CG4599|FBan0004599|CT14862|FBan0004599 99% Plus / Plus *F bs08f11.y1 AI945074 singleton GH26101.3prime 99% Plus / Minus none none *F bs08f12.y1 AI945075 Contig369 none none *F bs08g01.y1 AI945076 singleton CG14589|FBan0014589|CT34334|FBan0014589 99% Plus / Plus *F bs08g02.y1 AI945077 Contig226 CG7712|FBan0007712|CT23487|FBan0007712 98% Plus / Plus *F bs08g03.y1 AI945078 Contig520 none none *F bs08g04.y1 AI945079 Contig384 CG3345|FBan0003345|CT36339|FBan0003345 99% Plus / Minus *F bs08g05.y1 AI945080 singleton CG9268|FBan0009268|CT26410|FBan0009268 99% Plus / Plus *F bs08g06.y1 AI945081 singleton CG2533|FBan0002533|CT8521|FBan0002533 99% Plus / Plus *F bs08g07.y1 AI945082 singleton LD29816.5prime 100% Plus / Plus CG6238|FBan0006238|CT19498|FBan0006238 100% Plus / Plus *F bs08g08.y1 AI945083 singleton SD01779.5prime 100% Plus / Plus CG12360|FBan0012360|CT24046|FBan0012360 100% Plus / Plus *F bs08g09.y1 AI945084 Contig544 LD27287.3prime 99% Plus / Minus CG8059|FBan0008059|CT8064|FBan0008059 100% Plus / Plus *F bs08g10.y1 AI945085 singleton CG11578|FBan0011578|CT36508|FBan0011578 96% Plus / Plus *F bs08g11.y1 AI945086 singleton LD41258.5prime 99% Plus / Plus CG2238|FBan0002238|CT7410|FBan0002238 99% Plus / Plus *F bs08g12.y1 AI945087 singleton none none *F bs08h01.y1 AI945088 Contig421 CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs08h02.y1 AI945089 singleton GH10346.5prime 99% Plus / Plus CG6130|FBan0006130|CT19262|FBan0006130 97% Plus / Plus *F bs08h03.y1 AI945090 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs08h04.y1 AI945091 Contig514 GH14906.5prime 99% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 99% Plus / Plus *F bs08h05.y1 AI945092 singleton LP12207.3prime 99% Plus / Minus CG6512|FBan0006512|CT20265|FBan0006512 100% Plus / Plus *F bs08h06.y1 AI945093 Contig368 GH10330.5prime 98% Plus / Plus CG3494|FBan0003494|CT11751|FBan0003494 98% Plus / Plus *F bs08h07.y1 AI945094 singleton LD46512.5prime 100% Plus / Plus CG6355|FBan0006355|CT19856|FBan0006355 100% Plus / Plus *F bs08h08.y1 AI945095 singleton CG17417|FBan0017417|CT38455|FBan0017417 93% Plus / Plus *F bs08h09.y1 AI945096 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 98% Plus / Plus *F bs08h10.y1 AI945097 singleton none none *F bs08h11.y1 AI945098 Contig507 LD26935.5prime 99% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs08h12.y1 AI945099 singleton none none *F bs09a01.y1 AI945100 singleton GH14048.5prime 100% Plus / Plus CG7131|FBan0007131|CT22049|FBan0007131 100% Plus / Plus *F bs09a02.y1 AI945101 Contig516 GH12755.5prime 100% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 99% Plus / Plus *F bs09a04.y1 AI945102 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 96% Plus / Plus *F bs09a05.y1 pending Contig531 GH04958.5prime 97% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 97% Plus / Plus *F bs09a07.y1 AI945103 Contig474 GH11908.5prime 97% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 97% Plus / Plus *F bs09a08.y1 AI945104 Contig518 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 98% Plus / Plus *F bs09a09.y1 AI945105 Contig534 none none *F bs09a10.y1 AI945106 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs09a11.y1 AI945107 singleton none none *F bs09a12.y1 AI945108 Contig544 none none *F bs09b01.y1 AI945109 singleton CG1826|FBan0001826|CT5456|FBan0001826 100% Plus / Plus *F bs09b02.y1 AI945110 Contig507 GM04405.5prime 100% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs09b03.y1 AI945111 Contig437 CG3387|FBan0003387|CT11303|FBan0003387 99% Plus / Plus *F bs09b04.y1 AI945112 Contig364 GM01939.5prime 98% Plus / Plus CG9992|FBan0009992|CT28165|FBan0009992 98% Plus / Plus *F bs09b05.y1 AI945113 Contig516 GH12755.5prime 100% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 100% Plus / Plus *F bs09b06.y1 AI945114 Contig472 GH22559.5prime 83% Plus / Plus CG9129|FBan0009129|CT8135|FBan0009129 99% Plus / Plus *F bs09b07.y1 AI945115 Contig510 GH21762.5prime 99% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 99% Plus / Plus *F bs09b08.y1 AI945116 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs09b09.y1 AI945117 Contig542 GH21803.5prime 96% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs09b11.y1 AI945118 Contig486 CG7268|FBan0007268|CT22431|FBan0007268 97% Plus / Plus *F bs09b12.y1 AI945119 singleton none none *F bs09c01.y1 AI945120 singleton CG12069|FBan0012069|CT4640|FBan0012069 99% Plus / Plus *F bs09c02.y1 AI945121 Contig488 GH27336.5prime 99% Plus / Plus none none *F bs09c03.y1 AI945122 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs09c04.y1 AI945123 Contig506 GH20038.5prime 100% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 100% Plus / Plus *F bs09c05.y1 AI945124 singleton LD05445.5prime 97% Plus / Plus CG2224|FBan0002224|CT6461|FBan0002224 98% Plus / Plus *F bs09c06.y1 AI945125 Contig534 none none *F bs09c07.y1 AI945126 singleton GM01409.5prime 95% Plus / Plus CG8198|FBan0008198|CT24415|FBan0008198 96% Plus / Plus *F bs09c08.y1 AI945127 singleton GH26674.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs09c09.y1 AI945128 Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs09c10.y1 AI945129 Contig428 LP09525.5prime 100% Plus / Plus CG15219|FBan0015219|CT35155|FBan0015219 100% Plus / Plus *F bs09c11.y1 AI945130 Contig303 HL03878.5prime 99% Plus / Plus CG10089|FBan0010089|CT28377|FBan0010089 99% Plus / Plus *F bs09c12.y1 AI945131 singleton none none *F bs09d01.y1 AI945132 singleton CG11226|FBan0011226|CT23684|FBan0011226 100% Plus / Plus *F bs09d02.y1 AI945133 Contig225 GM08410.5prime 100% Plus / Plus CG1469|FBan0001469|CT3604|FBan0001469 99% Plus / Plus *F bs09d04.y1 AI945134 Contig544 GH10815.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs09d05.y1 AI945135 Contig538 GH09790.5prime 100% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 100% Plus / Plus *F bs09d06.y1 AI945136 Contig529 LP04643.5prime 98% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 95% Plus / Plus *F bs09d07.y1 AI945137 Contig277 GH14656.5prime 99% Plus / Minus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Minus *F bs09d08.y1 AI945138 singleton HL02035.5prime 100% Plus / Plus CG8364|FBan0008364|CT24643|FBan0008364 100% Plus / Plus *F bs09d09.y1 AI945139 singleton GH23615.5prime 100% Plus / Plus CG1848|FBan0001848|CT5560|FBan0001848 100% Plus / Plus *F bs09d10.y1 AI945140 Contig521 GH15271.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs09d11.y1 AI945141 singleton GH01933.5prime 99% Plus / Plus CG3955|FBan0003955|CT13156|FBan0003955 99% Plus / Plus *F bs09d12.y1 AI945142 Contig538 GH09790.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs09e01.y1 AI945143 Contig498 CG8626|FBan0008626|CT25017|FBan0008626 99% Plus / Plus *F bs09e02.y1 AI945144 singleton none none *F bs09e03.y1 AI945145 singleton none none *F bs09e04.y1 AI945146 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs09e05.y1 AI945147 Contig525 GH21964.5prime 99% Plus / Plus CG11546|FBan0011546|CT36451|FBan0011546 99% Plus / Plus *F bs09e06.y1 AI945148 Contig445 LP10010.5prime 97% Plus / Plus CG1288|FBan0001288|CT2110|FBan0001288 100% Plus / Plus *F bs09e07.y1 AI945149 singleton none none *F bs09e08.y1 AI945150 singleton none none *F bs09e09.y1 AI945151 singleton none none *F bs09e10.y1 AI945152 Contig467 GH04277.5prime 99% Plus / Plus CG6541|FBan0006541|CT20379|FBan0006541 99% Plus / Plus *F bs09e11.y1 AI945153 singleton LD42941.5prime 100% Plus / Plus CG9423|FBan0009423|CT26681|FBan0009423 100% Plus / Plus *F bs09e12.y1 AI945154 singleton GM04879.5prime 99% Plus / Plus CG18642|FBan0018642|CT42330|FBan0018642 100% Plus / Plus *F bs09f01.y1 AI945155 singleton GH07192.5prime 98% Plus / Plus CG9222|FBan0009222|CT26342|FBan0009222 99% Plus / Plus *F bs09f02.y1 AI945156 Contig528 LP01076.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs09f03.y1 AI945157 singleton LD13887.5prime 99% Plus / Plus CG3628|FBan0003628|CT12077|FBan0003628 100% Plus / Plus *F bs09f04.y1 pending Contig221 CG10869|FBan0010869|CT30429|FBan0010869 98% Plus / Plus *F bs09f05.y1 AI945158 Contig502 GH01042.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs09f06.y1 AI945159 singleton none none *F bs09f07.y1 AI945160 singleton CG7257|FBan0007257|CT22391|FBan0007257 99% Plus / Plus *F bs09f08.y1 AI945161 Contig407 CG3399|FBan0003399|CT11427|FBan0003399 99% Plus / Plus *F bs09f09.y1 AI945162 singleton GH20124.5prime 100% Plus / Plus CG8489|FBan0008489|CT24829|FBan0008489 100% Plus / Plus *F bs09f10.y1 AI945163 Contig220 LD15654.5prime 100% Plus / Plus CG7082|FBan0007082|CT21883|FBan0007082 100% Plus / Plus *F bs09f11.y1 AI945164 Contig504 GH08122.3prime 100% Plus / Minus CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs09f12.y1 AI945165 singleton GM02890.5prime 100% Plus / Plus CG15081|FBan0015081|CT34956|FBan0015081 99% Plus / Plus *F bs09g01.y1 pending Contig409 none none *F bs09g02.y1 AI945166 Contig218 GH17527.5prime 89% Plus / Plus CG5075|FBan0005075|CT16217|FBan0005075 99% Plus / Plus *F bs09g03.y1 AI945167 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 99% Plus / Plus *F bs09g04.y1 AI945168 Contig506 GH20038.5prime 100% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 100% Plus / Plus *F bs09g05.y1 AI945169 Contig471 none none *F bs09g06.y1 AI945170 Contig490 LP05614.5prime 100% Plus / Plus CG13263|FBan0013263|CT32547|FBan0013263 100% Plus / Plus *F bs09g07.y1 AI945171 singleton GH08440.5prime 99% Plus / Plus CG15224|FBan0015224|CT35161|FBan0015224 97% Plus / Plus *F bs09g08.y1 AI945172 Contig367 none none *F bs09g09.y1 AI945173 Contig542 GH21803.5prime 96% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs09g10.y1 AI945174 Contig217 CG13164|FBan0013164|CT32405|FBan0013164 100% Plus / Plus *F bs09g11.y1 AI945175 Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs09g12.y1 AI945176 Contig420 GH12084.3prime 100% Plus / Minus CG6105|FBan0006105|CT19171|FBan0006105 100% Plus / Plus *F bs09h01.y1 pending singleton none none *F bs09h02.y1 AI945177 Contig457 GH08383.5prime 99% Plus / Plus CG6255|FBan0006255|CT19574|FBan0006255 100% Plus / Plus *F bs09h03.y1 AI945178 singleton CG1531|FBan0001531|CT3957|FBan0001531 99% Plus / Plus *F bs09h04.y1 pending Contig284 CG3268|FBan0003268|CT9209|FBan0003268 99% Plus / Plus *F bs09h05.y1 AI945179 singleton GH27756.5prime 100% Plus / Plus CG4955|FBan0004955|CT15900|FBan0004955 100% Plus / Plus *F bs09h06.y1 AI945180 singleton GH04805.5prime 99% Plus / Plus CG14235|FBan0014235|CT33854|FBan0014235 99% Plus / Plus *F bs09h07.y1 AI945181 Contig304 CG16984|FBan0016984|CT34790|FBan0016984 99% Plus / Plus *F bs09h08.y1 AI945182 Contig265 CG2964|FBan0002964|CT10035|FBan0002964 99% Plus / Plus *F bs09h09.y1 AI945183 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs09h10.y1 AI945184 Contig540 GH01761.5prime 100% Plus / Plus none none *F bs09h11.y1 AI945185 singleton HL01424.5prime 99% Plus / Plus CG8188|FBan0008188|CT24397|FBan0008188 99% Plus / Plus *F bs09h12.y1 AI945186 singleton CG10930|FBan0010930|CT30615|FBan0010930 100% Plus / Plus *F bs10a02.y1 AI945187 Contig533 GH21951.5prime 98% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs10a03.y1 AI945188 Contig215 none none *F bs10a04.y1 AI945189 Contig366 CK01909.contig 90% Plus / Plus CG6756|FBan0006756|CT20965|FBan0006756 100% Plus / Plus *F bs10a05.y1 AI945190 Contig413 LP02950.5prime 99% Plus / Plus CG8251|FBan0008251|CT2835|FBan0008251 100% Plus / Plus *F bs10a06.y1 AI945191 Contig365 CK01274.contig 99% Plus / Plus CG5103|FBan0005103|CT16369|FBan0005103 100% Plus / Plus *F bs10a07.y1 pending Contig264 CG8526|FBan0008526|CT24903|FBan0008526 99% Plus / Plus *F bs10a08.y1 AI945192 Contig528 GH13107.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs10a09.y1 AI945193 singleton CG1819|FBan0001819|CT5500|FBan0001819 100% Plus / Plus *F bs10a10.y1 AI945194 Contig364 GM01939.5prime 99% Plus / Plus CG9992|FBan0009992|CT28165|FBan0009992 100% Plus / Plus *F bs10a11.y1 AI945195 singleton GM03135.5prime 95% Plus / Plus none none *F bs10a12.y1 AI945196 singleton HL01252.5prime 99% Plus / Plus CG9218|FBan0009218|CT26312|FBan0009218 99% Plus / Plus *F bs10b01.y1 AI945197 Contig476 LP11595.5prime 100% Plus / Plus CG3752|FBan0003752|CT12541|FBan0003752 100% Plus / Plus *F bs10b02.y1 AI945198 Contig536 LP09196.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs10b03.y1 AI945199 Contig539 GH27943.3prime 99% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs10b04.y1 AI945200 Contig514 GH24336.5prime 100% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 100% Plus / Plus *F bs10b05.y1 AI945201 Contig506 GH20038.5prime 99% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 99% Plus / Plus *F bs10b06.y1 AI945202 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs10b07.y1 AI945203 Contig213 GH02337.5prime 100% Plus / Plus CG4046|FBan0004046|CT13148|FBan0004046 100% Plus / Plus *F bs10b08.y1 AI945204 Contig469 CG8732|FBan0008732|CT25221|FBan0008732 100% Plus / Minus *F bs10b09.y1 AI945205 Contig396 CG11392|FBan0011392|CT31794|FBan0011392 100% Plus / Plus *F bs10b10.y1 AI945206 Contig437 none none *F bs10b11.y1 AI945207 Contig212 GH25042.5prime 99% Plus / Plus CG5885|FBan0005885|CT18469|FBan0005885 99% Plus / Plus *F bs10b12.y1 AI945208 Contig484 CG5538|FBan0005538|CT17502|FBan0005538 100% Plus / Plus *F bs10c01.y1 AI945209 singleton LD41117.5prime 100% Plus / Plus CG1624|FBan0001624|CT4340|FBan0001624 100% Plus / Plus *F bs10c02.y1 AI945210 Contig500 LD35818.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs10c03.y1 AI945211 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs10c04.y1 AI945212 Contig363 CG5017|FBan0005017|CT16116|FBan0005017 100% Plus / Plus *F bs10c05.y1 AI945213 singleton LD20109.5prime 99% Plus / Plus CG2168|FBan0002168|CT7064|FBan0002168 99% Plus / Plus *F bs10c06.y1 AI945214 singleton CG9296|FBan0009296|CT26479|FBan0009296 100% Plus / Plus *F bs10c07.y1 AI945215 Contig211 CG1287|FBan0001287|CT2727|FBan0001287 100% Plus / Plus *F bs10c08.y1 AI945216 singleton CG1309|FBan0001309|CT2881|FBan0001309 100% Plus / Plus *F bs10c09.y1 AI945217 Contig536 GM04752.5prime 100% Plus / Minus none none *F bs10c10.y1 AI945218 Contig527 GH25431.5prime 98% Plus / Plus CG1835|FBan0001835|CT5600|FBan0001835 99% Plus / Plus *F bs10c11.y1 AI945219 Contig535 GH03745.5prime 100% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 100% Plus / Plus *F bs10c12.y1 AI945220 Contig361 GH11850.5prime 96% Plus / Plus CG4479|FBan0004479|CT14582|FBan0004479 98% Plus / Plus *F bs10d02.y1 AI945221 singleton CG4213|FBan0004213|CT13886|FBan0004213 99% Plus / Plus *F bs10d03.y1 AI945222 Contig210 none none *F bs10d04.y1 AI945223 Contig449 CG12201|FBan0012201|CT10663|FBan0012201 99% Plus / Plus *F bs10d05.y1 AI945224 singleton CG5327|FBan0005327|CT16944|FBan0005327 98% Plus / Plus *F bs10d06.y1 AI945225 Contig209 GH28557.5prime 100% Plus / Plus CG7770|FBan0007770|CT23626|FBan0007770 100% Plus / Plus *F bs10d07.y1 AI945226 singleton GM09133.5prime 99% Plus / Plus CG8385|FBan0008385|CT31328|FBan0008385 99% Plus / Plus *F bs10d08.y1 AI945227 Contig409 none none *F bs10d09.y1 AI945228 Contig422 SD07466.5prime 99% Plus / Plus CG9323|FBan0009323|CT3608|FBan0009323 100% Plus / Plus *F bs10d10.y1 AI945229 Contig531 GH04958.5prime 100% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs10d11.y1 AI945230 Contig485 none none *F bs10d12.y1 pending Contig208 none none *F bs10e01.y1 AI945231 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs10e02.y1 AI945232 Contig526 CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs10e03.y1 AI945233 singleton GH28314.5prime 82% Plus / Plus CG4988|FBan0004988|CT16002|FBan0004988 99% Plus / Plus *F bs10e04.y1 AI945234 Contig207 CG13477|FBan0013477|CT32842|FBan0013477 99% Plus / Plus *F bs10e05.y1 AI945235 Contig486 CG7268|FBan0007268|CT22431|FBan0007268 97% Plus / Plus *F bs10e06.y1 AI945236 Contig544 GH25863.5prime 98% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 98% Plus / Plus *F bs10e07.y1 AI945237 singleton CG11731|FBan0011731|CT32186|FBan0011731 100% Plus / Plus *F bs10e08.y1 AI945238 Contig506 LP10995.5prime 99% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 99% Plus / Plus *F bs10e09.y1 AI945239 singleton CG8958|FBan0008958|CT25736|FBan0008958 100% Plus / Plus *F bs10e10.y1 AI945240 Contig525 CG17935|FBan0017935|CT39964|FBan0017935 96% Plus / Plus *F bs10e11.y1 AI945241 singleton none none *F bs10e12.y1 AI945242 singleton none none *F bs10f01.y1 AI945243 Contig443 CG10734|FBan0010734|CT30089|FBan0010734 97% Plus / Plus *F bs10f02.y1 AI945244 singleton GH10978.3prime 100% Plus / Plus CG4169|FBan0004169|CT13760|FBan0004169 100% Plus / Plus *F bs10f03.y1 AI945245 singleton GH11392.3prime 98% Plus / Minus CG3291|FBan0003291|CT10981|FBan0003291 98% Plus / Plus *F bs10f04.y1 AI945246 Contig206 CG6569|FBan0006569|CT20440|FBan0006569 100% Plus / Plus *F bs10f05.y1 AI945247 Contig403 LP10147.5prime 99% Plus / Plus CG12227|FBan0012227|CT12747|FBan0012227 99% Plus / Plus *F bs10f06.y1 AI945248 Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs10f07.y1 AI945249 Contig493 CG12799|FBan0012799|CT38809|FBan0012799 100% Plus / Plus *F bs10f08.y1 AI945250 Contig205 CG5043|FBan0005043|CT16181|FBan0005043 100% Plus / Plus *F bs10f09.y1 AI945251 Contig534 none none *F bs10f10.y1 AI945252 singleton GM04664.5prime 100% Plus / Plus CG8097|FBan0008097|CT24230|FBan0008097 100% Plus / Plus *F bs10f11.y1 AI945253 singleton GM06266.5prime 97% Plus / Plus CG5851|FBan0005851|CT18355|FBan0005851 97% Plus / Plus *F bs10f12.y1 AI945254 singleton CG7370|FBan0007370|CT22695|FBan0007370 92% Plus / Plus *F bs10g01.y1 AI945255 Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs10g02.y1 AI945256 Contig512 LP07747.5prime 98% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 98% Plus / Plus *F bs10g03.y1 AI945257 Contig360 GH23889.5prime 98% Plus / Plus CG5106|FBan0005106|CT15693|FBan0005106 99% Plus / Plus *F bs10g04.y1 AI945258 Contig492 GH15883.5prime 99% Plus / Plus CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs10g05.y1 AI945259 Contig370 LD24941.3prime 98% Plus / Plus CG4599|FBan0004599|CT14862|FBan0004599 98% Plus / Minus *F bs10g06.y1 AI945260 singleton LD21494.5prime 97% Plus / Minus CG11988|FBan0011988|CT32118|FBan0011988 99% Plus / Minus *F bs10g07.y1 AI945261 Contig532 CG7024|FBan0007024|CT21720|FBan0007024 99% Plus / Plus *F bs10g08.y1 AI945262 Contig544 none none *F bs10g09.y1 AI945263 singleton CG5998|FBan0005998|CT18844|FBan0005998 100% Plus / Plus *F bs10g10.y1 AI945264 Contig490 LP12384.5prime 100% Plus / Plus CG13263|FBan0013263|CT32547|FBan0013263 100% Plus / Plus *F bs10g11.y1 AI945265 singleton GH06785.5prime 100% Plus / Plus CG7415|FBan0007415|CT41120|FBan0007415 100% Plus / Plus *F bs10g12.y1 AI945266 singleton SD05462.5prime 99% Plus / Plus CG5887|FBan0005887|CT17592|FBan0005887 99% Plus / Plus *F bs10h01.y1 AI945267 singleton CG10077|FBan0010077|CT28357|FBan0010077 100% Plus / Plus *F bs10h02.y1 AI945268 singleton CG10862|FBan0010862|CT30405|FBan0010862 96% Plus / Plus *F bs10h03.y1 AI945269 Contig533 GH09435.5prime 100% Plus / Plus none none *F bs10h04.y1 AI945270 Contig529 GH08122.3prime 94% Plus / Minus CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs10h05.y1 AI945271 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 98% Plus / Plus *F bs10h06.y1 AI945272 singleton LD18077.5prime 99% Plus / Plus CG8817|FBan0008817|CT25378|FBan0008817 100% Plus / Plus *F bs10h07.y1 AI945273 singleton none none *F bs10h09.y1 AI945274 Contig464 CG14297|FBan0014297|CT33927|FBan0014297 99% Plus / Plus *F bs10h11.y1 AI945276 singleton CG3964|FBan0003964|CT13163|FBan0003964 97% Plus / Plus *F bs10h12.y1 AI945277 singleton none none *F bs11a01.y1 AI945278 singleton CG7856|FBan0007856|CT3630|FBan0007856 99% Plus / Plus *F bs11a02.y1 AI945279 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs11a03.y1 AI945280 Contig204 LP04746.5prime 96% Plus / Plus CG2297|FBan0002297|CT7648|FBan0002297 96% Plus / Plus *F bs11a04.y1 AI945281 singleton GM06533.5prime 98% Plus / Plus CG7768|FBan0007768|CT23620|FBan0007768 99% Plus / Plus *F bs11a05.y1 AI945282 singleton none none *F bs11a06.y1 AI945283 Contig540 none none *F bs11a07.y1 AI945284 Contig203 CG1631|FBan0001631|CT4408|FBan0001631 99% Plus / Plus *F bs11a08.y1 AI945285 Contig203 GH23328.5prime 100% Plus / Plus CG17383|FBan0017383|CT38390|FBan0017383 100% Plus / Plus *F bs11a09.y1 AI945286 Contig473 GH11857.5prime 100% Plus / Plus CG14290|FBan0014290|CT33919|FBan0014290 100% Plus / Plus *F bs11a10.y1 AI945287 singleton CG17736|FBan0017736|CT39309|FBan0017736 99% Plus / Plus *F bs11a11.y1 AI945288 Contig484 CG5538|FBan0005538|CT17502|FBan0005538 99% Plus / Plus *F bs11a12.y1 AI945289 Contig512 LP07747.5prime 98% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 98% Plus / Plus *F bs11b01.y1 AI945290 Contig441 GH26265.5prime 96% Plus / Plus none none *F bs11b02.y1 AI945291 Contig539 CG6481|FBan0006481|CT20192|FBan0006481 99% Plus / Plus *F bs11b03.y1 AI945292 Contig544 LD24410.3prime 99% Plus / Minus CG8963|FBan0008963|CT25756|FBan0008963 100% Plus / Plus *F bs11b05.y1 AI945293 singleton CG12784|FBan0012784|CT36897|FBan0012784 100% Plus / Plus *F bs11b06.y1 AI945294 Contig511 GH22851.5prime 98% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs11b07.y1 AI945295 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs11b08.y1 AI945296 Contig491 GH18255.5prime 100% Plus / Plus CG8136|FBan0008136|CT24268|FBan0008136 99% Plus / Plus *F bs11b09.y1 AI945297 Contig358 GH25818.5prime 99% Plus / Plus CG7815|FBan0007815|CT23740|FBan0007815 99% Plus / Plus *F bs11b10.y1 AI945298 Contig202 LP10575.3prime 99% Plus / Plus none none *F bs11b11.y1 AI945299 Contig356 none none *F bs11b12.y1 AI945300 singleton GH19351.5prime 100% Plus / Plus CG7283|FBan0007283|CT22467|FBan0007283 100% Plus / Plus *F bs11c01.y1 AI945301 singleton LD37778.5prime 99% Plus / Plus CG2212|FBan0002212|CT7332|FBan0002212 99% Plus / Plus *F bs11c02.y1 AI945302 Contig544 GH20124.5prime 100% Plus / Plus CG8489|FBan0008489|CT24829|FBan0008489 100% Plus / Plus *F bs11c03.y1 AI945303 Contig536 GH02210.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs11c04.y1 AI945304 Contig458 GH08656.5prime 99% Plus / Plus CG16758|FBan0016758|CT8044|FBan0016758 99% Plus / Plus *F bs11c05.y1 pending singleton GH09876.3prime 96% Plus / Plus CG10650|FBan0010650|CT29808|FBan0010650 96% Plus / Minus *F bs11c06.y1 AI945305 Contig443 CG10734|FBan0010734|CT30089|FBan0010734 98% Plus / Plus *F bs11c07.y1 AI945306 Contig522 LP09246.5prime 99% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 97% Plus / Plus *F bs11c08.y1 AI945307 Contig201 CG8397|FBan0008397|CT18265|FBan0008397 100% Plus / Plus *F bs11c09.y1 AI945308 Contig466 GH03466.5prime 96% Plus / Plus CG2267|FBan0002267|CT7048|FBan0002267 97% Plus / Plus *F bs11c11.y1 AI945309 Contig375 GH11292.5prime 100% Plus / Plus CG14981|FBan0014981|CT34830|FBan0014981 100% Plus / Plus *F bs11c12.y1 AI945310 Contig522 LP09246.5prime 99% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 100% Plus / Plus *F bs11d01.y1 AI945311 Contig432 LD38044.5prime 100% Plus / Plus CG8938|FBan0008938|CT25660|FBan0008938 100% Plus / Plus *F bs11d02.y1 AI945312 Contig537 GH19032.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs11d03.y1 AI945313 Contig200 none none *F bs11d04.y1 AI945314 singleton CG9240|FBan0009240|CT26394|FBan0009240 100% Plus / Plus *F bs11d05.y1 AI945315 Contig353 GM13444.5prime 98% Plus / Plus CG10045|FBan0010045|CT28269|FBan0010045 100% Plus / Plus *F bs11d06.y1 AI945316 Contig528 GH13107.5prime 99% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs11d07.y1 AI945317 Contig366 HL03142.5prime 98% Plus / Plus CG6756|FBan0006756|CT20965|FBan0006756 98% Plus / Plus *F bs11d08.y1 AI945318 Contig491 GH17727.5prime 99% Plus / Plus CG6332|FBan0006332|CT19816|FBan0006332 99% Plus / Plus *F bs11d09.y1 AI945319 Contig387 GH13802.5prime 97% Plus / Plus CG1314|FBan0001314|CT2886|FBan0001314 99% Plus / Plus *F bs11d10.y1 AI945320 Contig358 GH20409.5prime 99% Plus / Plus CG3557|FBan0003557|CT11964|FBan0003557 99% Plus / Plus *F bs11d12.y1 AI945321 singleton LD08974.5prime 99% Plus / Plus CG13777|FBan0013777|CT33265|FBan0013777 99% Plus / Plus *F bs11e01.y1 AI945322 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 100% Plus / Plus *F bs11e02.y1 AI945323 Contig428 LP09525.5prime 100% Plus / Plus CG15219|FBan0015219|CT35155|FBan0015219 100% Plus / Plus *F bs11e03.y1 AI945324 Contig480 GH15272.5prime 99% Plus / Plus CG1999|FBan0001999|CT6363|FBan0001999 99% Plus / Plus *F bs11e04.y1 AI945325 Contig352 GH17362.5prime 100% Plus / Plus CG12313|FBan0012313|CT21286|FBan0012313 100% Plus / Plus *F bs11e05.y1 AI945326 Contig204 GH24174.5prime 98% Plus / Plus CG2297|FBan0002297|CT7648|FBan0002297 98% Plus / Plus *F bs11e06.y1 AI945327 Contig542 GH26674.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs11e07.y1 AI945328 Contig426 none none *F bs11e08.y1 AI945329 Contig544 GH25863.5prime 98% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 98% Plus / Plus *F bs11e09.y1 AI945330 singleton CG9696|FBan0009696|CT27330|FBan0009696 99% Plus / Plus *F bs11e10.y1 pending Contig540 none none *F bs11e11.y1 AI945331 Contig495 GH19464.3prime 100% Plus / Minus CG9133|FBan0009133|CT10073|FBan0009133 100% Plus / Plus *F bs11e12.y1 AI945332 Contig453 GH09387.5prime 97% Plus / Plus CG9314|FBan0009314|CT26521|FBan0009314 99% Plus / Plus *F bs11f01.y1 AI945333 singleton GH15663.5prime 100% Plus / Plus CG7920|FBan0007920|CT6599|FBan0007920 100% Plus / Plus *F bs11f02.y1 AI945334 Contig351 GH16413.5prime 98% Plus / Plus CG4767|FBan0004767|CT15347|FBan0004767 99% Plus / Plus *F bs11f03.y1 AI945335 singleton LD30891.5prime 99% Plus / Plus CG3760|FBan0003760|CT12563|FBan0003760 99% Plus / Plus *F bs11f04.y1 AI945336 Contig290 CG11722|FBan0011722|CT31938|FBan0011722 100% Plus / Minus *F bs11f05.y1 AI945337 Contig350 CG17838|FBan0017838|CT39628|FBan0017838 100% Plus / Plus *F bs11f06.y1 AI945338 Contig489 GH01788.3prime 100% Plus / Minus CG10589|FBan0010589|CT29692|FBan0010589 99% Plus / Plus *F bs11f07.y1 AI945339 Contig544 GH03736.5prime 99% Plus / Plus CG3124|FBan0003124|CT10470|FBan0003124 99% Plus / Plus *F bs11f08.y1 AI945340 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs11f10.y1 AI945341 Contig348 GH13679.5prime 100% Plus / Plus CG17838|FBan0017838|CT39628|FBan0017838 100% Plus / Plus *F bs11f11.y1 AI945342 Contig347 none none *F bs11f12.y1 AI945343 Contig492 GH10725.5prime 98% Plus / Plus CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs11g01.y1 AI945344 singleton GH25035.5prime 99% Plus / Plus CG10429|FBan0010429|CT29288|FBan0010429 99% Plus / Plus *F bs11g02.y1 AI945345 Contig543 GH11850.5prime 100% Plus / Plus CG4478|FBan0004478|CT14576|FBan0004478 100% Plus / Plus *F bs11g03.y1 AI945346 Contig527 LP09058.5prime 98% Plus / Plus CG12896|FBan0012896|CT32041|FBan0012896 98% Plus / Plus *F bs11g05.y1 AI945347 singleton CG5182|FBan0005182|CT16567|FBan0005182 99% Plus / Plus *F bs11g06.y1 AI945348 Contig489 GH10553.5prime 99% Plus / Plus CG5045|FBan0005045|CT16189|FBan0005045 100% Plus / Plus *F bs11g07.y1 AI945349 Contig346 LD16755.5prime 99% Plus / Plus CG8448|FBan0008448|CT37123|FBan0008448 99% Plus / Plus *F bs11g08.y1 AI945350 Contig201 GH20356.3prime 100% Plus / Minus CG11359|FBan0011359|CT31686|FBan0011359 100% Plus / Minus *F bs11g09.y1 AI945351 Contig543 CG18130|FBan0018130|CT40826|FBan0018130 98% Plus / Plus *F bs11g10.y1 AI945352 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs11g12.y1 AI945353 Contig163 CG17118|FBan0017118|CT38042|FBan0017118 98% Plus / Plus *F bs11h01.y1 AI945354 Contig199 LP07070.5prime 99% Plus / Minus CG17146|FBan0017146|CT38082|FBan0017146 99% Plus / Plus *F bs11h02.y1 AI945355 Contig220 LD15654.5prime 100% Plus / Plus CG7082|FBan0007082|CT21883|FBan0007082 99% Plus / Plus *F bs11h03.y1 AI945356 Contig345 LP03157.5prime 99% Plus / Plus CG9412|FBan0009412|CT37417|FBan0009412 100% Plus / Plus *F bs11h04.y1 AI945357 Contig344 CG7094|FBan0007094|CT21551|FBan0007094 100% Plus / Plus *F bs11h05.y1 AI945358 Contig544 CG6060|FBan0006060|CT19011|FBan0006060 98% Plus / Plus *F bs11h06.y1 AI945359 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs11h07.y1 AI945360 singleton GH11336.3prime 100% Plus / Minus CG2038|FBan0002038|CT6589|FBan0002038 98% Plus / Plus *F bs11h08.y1 AI945361 Contig542 GH28631.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs11h09.y1 AI945362 singleton CG13476|FBan0013476|CT32841|FBan0013476 100% Plus / Plus *F bs11h10.y1 AI945363 Contig197 GH06775.5prime 100% Plus / Plus CG7251|FBan0007251|CT22371|FBan0007251 100% Plus / Plus *F bs11h11.y1 AI945364 Contig544 CG4636|FBan0004636|CT14962|FBan0004636 99% Plus / Plus *F bs11h12.y1 AI945365 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Minus *F bs12a12.y1 AI945366 singleton none none *F bs12b02.y1 AI945367 Contig375 GH11292.5prime 99% Plus / Plus CG14981|FBan0014981|CT34830|FBan0014981 99% Plus / Plus *F bs12b03.y1 AI945368 singleton CG1316|FBan0001316|CT2892|FBan0001316 98% Plus / Plus *F bs12b04.y1 AI945369 singleton CG17300|FBan0017300|CT38236|FBan0017300 99% Plus / Plus *F bs12b05.y1 AI945370 Contig420 GH12084.3prime 99% Plus / Minus CG6105|FBan0006105|CT19171|FBan0006105 99% Plus / Plus *F bs12b06.y1 AI945371 Contig440 CG13030|FBan0013030|CT32248|FBan0013030 97% Plus / Plus *F bs12b07.y1 AI945372 Contig518 GH25878.5prime 97% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs12b08.y1 AI945373 Contig538 GH09790.5prime 99% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 99% Plus / Plus *F bs12b09.y1 AI945374 singleton none none *F bs12b10.y1 AI945375 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs12b11.y1 AI945376 singleton LD34564.5prime 99% Plus / Minus CG9863|FBan0009863|CT27842|FBan0009863 100% Plus / Plus *F bs12b12.y1 AI945377 Contig473 CG3860|FBan0003860|CT12843|FBan0003860 99% Plus / Plus *F bs12c01.y1 AI945378 singleton GH19985.5prime 96% Plus / Plus CG12652|FBan0012652|CT35302|FBan0012652 97% Plus / Plus *F bs12c02.y1 AI945379 Contig196 LD11220.5prime 96% Plus / Plus CG3127|FBan0003127|CT10484|FBan0003127 99% Plus / Plus *F bs12c03.y1 AI945380 Contig544 CG12377|FBan0012377|CT25244|FBan0012377 99% Plus / Plus *F bs12c04.y1 AI945381 Contig157 none none *F bs12c05.y1 AI945382 Contig536 GH19031.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs12c06.y1 AI945383 Contig426 CK00552.5prime 98% Plus / Minus CG12908|FBan0012908|CT32053|FBan0012908 100% Plus / Minus *F bs12c07.y1 AI945384 Contig343 LP02441.5prime 99% Plus / Plus CG18370|FBan0018370|CT41755|FBan0018370 98% Plus / Plus *F bs12c08.y1 AI945385 Contig218 GH10636.5prime 83% Plus / Plus CG5075|FBan0005075|CT16217|FBan0005075 98% Plus / Plus *F bs12c09.y1 AI945386 Contig195 LP04811.5prime 100% Plus / Plus CG6998|FBan0006998|CT21660|FBan0006998 100% Plus / Plus *F bs12c10.y1 AI945387 Contig544 none none *F bs12c11.y1 AI945388 Contig521 GH15271.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs12c12.y1 AI945389 Contig363 CG5017|FBan0005017|CT16116|FBan0005017 99% Plus / Plus *F bs12d02.y1 AI945390 Contig417 GH14206.5prime 98% Plus / Plus CG2955|FBan0002955|CT10021|FBan0002955 98% Plus / Plus *F bs12d03.y1 AI945391 Contig194 LD38280.3prime 95% Plus / Minus none none *F bs12d04.y1 AI945392 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs12d05.y1 AI945393 Contig491 GH07879.5prime 98% Plus / Plus CG6332|FBan0006332|CT19816|FBan0006332 98% Plus / Plus *F bs12d06.y1 AI945394 Contig372 CG17010|FBan0017010|CT37753|FBan0017010 99% Plus / Plus *F bs12d07.y1 AI945395 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 98% Plus / Plus *F bs12d08.y1 AI945396 Contig421 CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs12d09.y1 AI945397 singleton CG3515|FBan0003515|CT11855|FBan0003515 99% Plus / Plus *F bs12d10.y1 AI945398 Contig516 GH12755.5prime 99% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 99% Plus / Plus *F bs12d12.y1 AI945399 Contig532 GH28719.5prime 98% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs12e01.y1 AI945400 singleton CG9254|FBan0009254|CT26022|FBan0009254 99% Plus / Plus *F bs12e02.y1 AI945401 Contig460 GH10940.5prime 97% Plus / Plus CG18662|FBan0018662|CT42585|FBan0018662 97% Plus / Minus *F bs12e03.y1 AI945402 singleton GH11476.5prime 99% Plus / Plus CG1979|FBan0001979|CT6207|FBan0001979 99% Plus / Plus *F bs12e04.y1 AI945403 Contig341 GH19562.5prime 98% Plus / Plus CG6412|FBan0006412|CT19988|FBan0006412 98% Plus / Plus *F bs12e05.y1 AI945404 Contig193 CG10635|FBan0010635|CT29790|FBan0010635 97% Plus / Plus *F bs12e06.y1 AI945405 Contig460 GH10940.5prime 97% Plus / Minus CG18662|FBan0018662|CT42585|FBan0018662 96% Plus / Plus *F bs12e07.y1 AI945406 Contig378 CG1137|FBan0001137|CT1904|FBan0001137 98% Plus / Plus *F bs12e08.y1 AI945407 Contig250 CG17944|FBan0017944|CT39966|FBan0017944 98% Plus / Plus *F bs12e09.y1 AI945408 Contig192 LD30571.5prime 99% Plus / Plus CG4466|FBan0004466|CT14530|FBan0004466 99% Plus / Plus *F bs12e10.y1 AI945409 Contig445 LP10010.5prime 98% Plus / Plus CG1288|FBan0001288|CT2110|FBan0001288 100% Plus / Plus *F bs12e11.y1 AI945410 Contig191 CG13243|FBan0013243|CT32493|FBan0013243 100% Plus / Minus *F bs12e12.y1 AI945411 singleton LP02676.5prime 99% Plus / Plus CG10360|FBan0010360|CT29100|FBan0010360 99% Plus / Plus *F bs12f01.y1 AI945412 Contig542 GH26674.5prime 96% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs12f02.y1 AI945413 singleton LP12441.5prime 99% Plus / Plus CG2841|FBan0002841|CT9712|FBan0002841 99% Plus / Plus *F bs12f03.y1 AI945414 singleton CG4073|FBan0004073|CT13542|FBan0004073 98% Plus / Plus *F bs12f04.y1 AI945415 Contig498 CG8626|FBan0008626|CT25017|FBan0008626 97% Plus / Plus *F bs12f05.y1 AI945416 singleton LD12712.5prime 99% Plus / Plus CG1483|FBan0001483|CT3677|FBan0001483 99% Plus / Plus *F bs12f06.y1 AI945417 Contig189 GH09682.5prime 98% Plus / Plus CG8209|FBan0008209|CT24423|FBan0008209 99% Plus / Plus *F bs12f07.y1 AI945418 singleton none none *F bs12f08.y1 AI945419 Contig188 GH02287.5prime 98% Plus / Plus CG2076|FBan0002076|CT6605|FBan0002076 99% Plus / Plus *F bs12f10.y1 AI945420 Contig200 CG4218|FBan0004218|CT11693|FBan0004218 99% Plus / Plus *F bs12f11.y1 AI945421 Contig490 LP05614.5prime 99% Plus / Plus CG13263|FBan0013263|CT32547|FBan0013263 99% Plus / Plus *F bs12f12.y1 AI945422 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs12g01.y1 AI945423 Contig483 CG15200|FBan0015200|CT35127|FBan0015200 99% Plus / Plus *F bs12g02.y1 AI945424 Contig481 CG5051|FBan0005051|CT16124|FBan0005051 100% Plus / Plus *F bs12g03.y1 AI945425 Contig529 LP04643.5prime 97% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 96% Plus / Plus *F bs12g04.y1 AI945426 singleton CG7211|FBan0007211|CT22239|FBan0007211 97% Plus / Plus *F bs12g05.y1 AI945427 Contig406 GH05530.5prime 99% Plus / Plus CG17567|FBan0017567|CT35086|FBan0017567 99% Plus / Plus *F bs12g06.y1 AI945428 Contig524 LP07256.5prime 99% Plus / Plus CG9410|FBan0009410|CT9155|FBan0009410 99% Plus / Plus *F bs12g07.y1 AI945429 Contig340 GH01027.5prime 99% Plus / Plus CG10124|FBan0010124|CT28485|FBan0010124 99% Plus / Plus *F bs12g08.y1 AI945430 Contig527 none none *F bs12g09.y1 AI945431 Contig187 LP07146.5prime 98% Plus / Plus CG9983|FBan0009983|CT37548|FBan0009983 99% Plus / Plus *F bs12g10.y1 AI945432 singleton LP07570.5prime 98% Plus / Plus CG11001|FBan0011001|CT30803|FBan0011001 98% Plus / Plus *F bs12g11.y1 AI945433 singleton LD26534.5prime 98% Plus / Plus CG10593|FBan0010593|CT29700|FBan0010593 98% Plus / Plus *F bs12g12.y1 AI945434 Contig473 GH11857.5prime 100% Plus / Plus CG14290|FBan0014290|CT41827|FBan0014290 100% Plus / Plus *F bs12h01.y1 AI945435 Contig186 GH23789.3prime 96% Plus / Minus CG10120|FBan0010120|CT38328|FBan0010120 96% Plus / Plus *F bs12h02.y1 pending singleton CG2774|FBan0002774|CT9125|FBan0002774 98% Plus / Plus *F bs12h03.y1 AI945436 singleton CG6612|FBan0006612|CT20570|FBan0006612 99% Plus / Plus *F bs12h04.y1 pending Contig477 none none *F bs12h05.y1 AI945437 singleton GH12064.5prime 98% Plus / Plus CG3860|FBan0003860|CT12843|FBan0003860 98% Plus / Plus *F bs12h06.y1 AI945438 Contig386 HL01913.5prime 96% Plus / Plus CG1225|FBan0001225|CT2308|FBan0001225 98% Plus / Plus *F bs12h07.y1 AI945439 Contig536 LP09170.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs12h08.y1 AI945440 Contig476 HL01203.5prime 99% Plus / Plus CG3752|FBan0003752|CT12541|FBan0003752 99% Plus / Plus *F bs12h09.y1 AI945441 Contig185 LD26964.5prime 100% Plus / Plus CG1837|FBan0001837|CT5608|FBan0001837 100% Plus / Plus *F bs12h10.y1 AI945442 Contig184 CG8244|FBan0008244|CT23727|FBan0008244 100% Plus / Plus *F bs12h11.y1 AI945443 Contig338 SD02144.5prime 99% Plus / Plus CG10691|FBan0010691|CT29956|FBan0010691 98% Plus / Plus *F bs12h12.y1 AI945444 singleton CG12307|FBan0012307|CT20690|FBan0012307 99% Plus / Plus *F bs13a01.y1 AI945445 singleton GH05955.5prime 99% Plus / Plus CG12070|FBan0012070|CT4748|FBan0012070 99% Plus / Plus *F bs13a02.y1 AI945446 singleton GH13450.5prime 99% Plus / Plus CG12389|FBan0012389|CT26006|FBan0012389 99% Plus / Plus *F bs13a03.y1 AI945447 singleton LD41155.5prime 100% Plus / Plus CG12746|FBan0012746|CT8919|FBan0012746 99% Plus / Plus *F bs13a04.y1 AI945448 Contig321 GH19896.3prime 100% Plus / Minus CG10664|FBan0010664|CT29876|FBan0010664 100% Plus / Plus *F bs13a05.y1 AI945449 Contig519 LD45863.5prime 99% Plus / Plus CG5825|FBan0005825|CT18281|FBan0005825 99% Plus / Plus *F bs13a06.y1 AI945450 Contig183 none none *F bs13a07.y1 AI945451 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs13a08.y1 AI945452 Contig528 GH07786.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs13a09.y1 AI945453 Contig182 GH07940.5prime 97% Plus / Plus CG11018|FBan0011018|CT30849|FBan0011018 97% Plus / Plus *F bs13a12.y1 pending singleton CG6923|FBan0006923|CT21444|FBan0006923 97% Plus / Plus *F bs13b01.y1 AI945454 Contig544 CG9868|FBan0009868|CT10456|FBan0009868 98% Plus / Plus *F bs13b02.y1 AI945455 Contig524 CG7466|FBan0007466|CT22933|FBan0007466 99% Plus / Plus *F bs13b03.y1 AI945456 Contig491 GH25937.5prime 98% Plus / Plus CG8136|FBan0008136|CT24268|FBan0008136 98% Plus / Plus *F bs13b04.y1 AI945457 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs13b05.y1 AI945458 Contig397 CG11861|FBan0011861|CT40006|FBan0011861 99% Plus / Plus *F bs13b06.y1 AI945459 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs13b07.y1 AI945460 singleton CG7707|FBan0007707|CT23481|FBan0007707 98% Plus / Plus *F bs13b08.y1 AI945461 singleton GH25765.5prime 99% Plus / Plus CG8257|FBan0008257|CT24479|FBan0008257 99% Plus / Plus *F bs13b09.y1 AI945462 Contig524 CG10234|FBan0010234|CT28767|FBan0010234 99% Plus / Plus *F bs13b10.y1 AI945463 Contig544 LD37577.5prime 98% Plus / Plus CG18621|FBan0018621|CT30607|FBan0018621 98% Plus / Plus *F bs13b11.y1 AI945464 Contig454 CG2164|FBan0002164|CT7072|FBan0002164 99% Plus / Plus *F bs13b12.y1 AI945465 Contig542 GH13953.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs13c01.y1 AI945466 singleton GH08818.5prime 99% Plus / Plus CG7772|FBan0007772|CT23646|FBan0007772 99% Plus / Plus *F bs13c02.y1 AI945467 singleton CG13520|FBan0013520|CT32891|FBan0013520 99% Plus / Minus *F bs13c03.y1 AI945468 singleton CG16782|FBan0016782|CT37311|FBan0016782 98% Plus / Plus *F bs13c04.y1 AI945469 singleton CG14740|FBan0014740|CT34534|FBan0014740 92% Plus / Plus *F bs13c05.y1 AI945470 Contig417 LP10240.5prime 98% Plus / Plus CG2955|FBan0002955|CT10021|FBan0002955 98% Plus / Plus *F bs13c06.y1 AI945471 Contig510 GH21762.5prime 99% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 99% Plus / Plus *F bs13c07.y1 AI945472 singleton CG4608|FBan0004608|CT14882|FBan0004608 98% Plus / Plus *F bs13c08.y1 AI945473 Contig448 LD38313.5prime 99% Plus / Minus CG1866|FBan0001866|CT5760|FBan0001866 99% Plus / Minus *F bs13c10.y1 AI945474 singleton none none *F bs13c11.y1 AI945475 Contig544 LP06279.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs13c12.y1 AI945476 Contig180 LD42191.5prime 99% Plus / Plus CG5514|FBan0005514|CT17470|FBan0005514 99% Plus / Plus *F bs13d01.y1 AI945477 singleton CG18371|FBan0018371|CT41759|FBan0018371 99% Plus / Plus *F bs13d02.y1 AI945478 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs13d03.y1 AI945479 Contig524 CG13891|FBan0013891|CT33424|FBan0013891 98% Plus / Plus *F bs13d04.y1 AI945480 singleton LD48011.5prime 99% Plus / Plus CG3662|FBan0003662|CT12281|FBan0003662 99% Plus / Plus *F bs13d05.y1 AI945481 Contig459 LP06931.5prime 100% Plus / Plus CG4323|FBan0004323|CT14141|FBan0004323 100% Plus / Plus *F bs13d06.y1 AI945482 Contig268 GH25314.5prime 100% Plus / Plus CG8654|FBan0008654|CT25029|FBan0008654 99% Plus / Plus *F bs13d07.y1 AI945483 Contig390 GH26259.5prime 100% Plus / Plus CG9975|FBan0009975|CT28093|FBan0009975 100% Plus / Plus *F bs13d08.y1 AI945484 Contig448 GH03956.5prime 99% Plus / Plus CG17650|FBan0017650|CT32531|FBan0017650 99% Plus / Plus *F bs13d09.y1 AI945485 Contig282 CG16954|FBan0016954|CT35806|FBan0016954 98% Plus / Plus *F bs13d10.y1 AI945486 singleton CG11253|FBan0011253|CT31419|FBan0011253 98% Plus / Plus *F bs13d11.y1 AI945487 Contig496 CK00537.5prime 98% Plus / Plus CG7441|FBan0007441|CT22887|FBan0007441 98% Plus / Plus *F bs13d12.y1 AI945488 Contig178 CG7886|FBan0007886|CT23840|FBan0007886 100% Plus / Plus *F bs13e01.y1 AI945489 singleton CG7922|FBan0007922|CT23918|FBan0007922 97% Plus / Plus *F bs13e02.y1 AI945490 singleton none none *F bs13e03.y1 AI945491 Contig278 CG15109|FBan0015109|CT42559|FBan0015109 100% Plus / Plus *F bs13e04.y1 AI945492 Contig257 GH09764.5prime 99% Plus / Plus CG14576|FBan0014576|CT34308|FBan0014576 100% Plus / Plus *F bs13e05.y1 AI945493 Contig246 GH20396.5prime 98% Plus / Plus CG3809|FBan0003809|CT12693|FBan0003809 98% Plus / Plus *F bs13e06.y1 AI945494 Contig520 none none *F bs13e07.y1 AI945495 Contig524 CG14512|FBan0014512|CT34232|FBan0014512 99% Plus / Plus *F bs13e08.y1 AI945496 Contig488 GH11373.5prime 100% Plus / Plus none none *F bs13e09.y1 AI945497 Contig527 none none *F bs13e10.y1 AI945498 singleton none none *F bs13e11.y1 AI945499 Contig539 GH27943.3prime 98% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs13e12.y1 AI945500 Contig508 LD23817.3prime 97% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs13f01.y1 AI945501 Contig174 CG18463|FBan0018463|CT33393|FBan0018463 98% Plus / Plus *F bs13f02.y1 AI945502 Contig518 GH25878.5prime 97% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs13f03.y1 AI945503 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs13f04.y1 AI945504 Contig173 CK01602.contig 95% Plus / Plus CG8245|FBan0008245|CT2430|FBan0008245 99% Plus / Plus *F bs13f05.y1 AI945505 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs13f06.y1 AI945506 singleton LD14164.5prime 98% Plus / Plus CG12567|FBan0012567|CT34324|FBan0012567 99% Plus / Plus *F bs13f07.y1 AI945507 Contig467 GH04277.5prime 99% Plus / Plus CG6541|FBan0006541|CT20379|FBan0006541 98% Plus / Plus *F bs13f08.y1 AI945508 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs13f09.y1 AI945509 singleton GM02126.5prime 100% Plus / Plus CG11958|FBan0011958|CT4036|FBan0011958 98% Plus / Plus *F bs13f10.y1 AI945510 singleton none none *F bs13f11.y1 AI945511 Contig172 CG5987|FBan0005987|CT18801|FBan0005987 99% Plus / Plus *F bs13f12.y1 AI945512 Contig515 LP07011.5prime 100% Plus / Plus none none *F bs13g01.y1 AI945513 Contig447 GH12486.5prime 95% Plus / Plus CG4375|FBan0004375|CT14270|FBan0004375 95% Plus / Plus *F bs13g02.y1 AI945514 Contig363 CG5017|FBan0005017|CT16116|FBan0005017 100% Plus / Plus *F bs13g03.y1 AI945515 Contig410 CG17946|FBan0017946|CT39984|FBan0017946 100% Plus / Plus *F bs13g04.y1 AI945516 Contig337 GH07823.3prime 99% Plus / Plus CG8430|FBan0008430|CT18945|FBan0008430 99% Plus / Minus *F bs13g05.y1 AI945517 singleton none none *F bs13g06.y1 AI945518 singleton CG2225|FBan0002225|CT7374|FBan0002225 96% Plus / Minus *F bs13g07.y1 AI945519 singleton CG10272|FBan0010272|CT28863|FBan0010272 100% Plus / Plus *F bs13g08.y1 AI945520 Contig413 CG6629|FBan0006629|CT20612|FBan0006629 99% Plus / Plus *F bs13g09.y1 AI945521 Contig194 CG2525|FBan0002525|CT8431|FBan0002525 100% Plus / Minus *F bs13g10.y1 AI945522 Contig402 LD04413.5prime 97% Plus / Minus CG3265|FBan0003265|CT37737|FBan0003265 98% Plus / Plus *F bs13g11.y1 AI945523 Contig418 LP09619.5prime 99% Plus / Plus CG10578|FBan0010578|CT29670|FBan0010578 99% Plus / Plus *F bs13g12.y1 AI945524 Contig448 GH24329.3prime 99% Plus / Minus CG4501|FBan0004501|CT14645|FBan0004501 99% Plus / Plus *F bs13h01.y1 AI945525 singleton SD09626.5prime 99% Plus / Minus CG6968|FBan0006968|CT21581|FBan0006968 99% Plus / Plus *F bs13h02.y1 AI945526 Contig398 CG11588|FBan0011588|CT22407|FBan0011588 99% Plus / Plus *F bs13h03.y1 AI945527 Contig355 LD41444.3prime 99% Plus / Minus CG8284|FBan0008284|CT24417|FBan0008284 99% Plus / Plus *F bs13h04.y1 AI945528 Contig544 none none *F bs13h05.y1 AI945529 singleton GH22559.5prime 100% Plus / Plus CG5467|FBan0005467|CT17334|FBan0005467 98% Plus / Plus *F bs13h06.y1 AI945530 Contig534 none none *F bs13h07.y1 AI945531 Contig542 GH26674.5prime 98% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs13h08.y1 AI945532 Contig193 CG9072|FBan0009072|CT26046|FBan0009072 99% Plus / Plus *F bs13h09.y1 AI945533 singleton SD08668.5prime 99% Plus / Plus CG5547|FBan0005547|CT37369|FBan0005547 99% Plus / Plus *F bs13h10.y1 AI945534 singleton CG5823|FBan0005823|CT18279|FBan0005823 100% Plus / Plus *F bs13h11.y1 AI945535 Contig359 CG12229|FBan0012229|CT12907|FBan0012229 100% Plus / Plus *F bs13h12.y1 AI945536 Contig540 none none *F bs14a01.y1 AI945537 Contig481 CG5051|FBan0005051|CT16124|FBan0005051 100% Plus / Plus *F bs14a02.y1 pending singleton LD32357.5prime 95% Plus / Plus CG12096|FBan0012096|CT5973|FBan0012096 95% Plus / Plus *F bs14a04.y1 AI945538 singleton GH07417.5prime 99% Plus / Plus CG2998|FBan0002998|CT4358|FBan0002998 100% Plus / Plus *F bs14a05.y1 AI945539 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs14a06.y1 pending Contig497 CG12917|FBan0012917|CT32063|FBan0012917 98% Plus / Plus *F bs14a07.y1 AI945540 singleton none none *F bs14a08.y1 AI945541 Contig523 LD23336.3prime 97% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs14a09.y1 AI945542 Contig308 CG6943|FBan0006943|CT21523|FBan0006943 100% Plus / Plus *F bs14a11.y1 AI945543 Contig164 GH09392.5prime 100% Plus / Plus CG10589|FBan0010589|CT29692|FBan0010589 100% Plus / Plus *F bs14a12.y1 AI945544 Contig514 GH14906.5prime 99% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 99% Plus / Plus *F bs14b01.y1 AI945545 singleton LD21673.3prime 99% Plus / Plus CG17718|FBan0017718|CT39287|FBan0017718 100% Plus / Minus *F bs14b02.y1 AI945546 singleton none none *F bs14b03.y1 AI945547 Contig544 CG10561|FBan0010561|CT29482|FBan0010561 98% Plus / Plus *F bs14b04.y1 AI945548 Contig162 LD13184.5prime 98% Plus / Minus CG6661|FBan0006661|CT20682|FBan0006661 100% Plus / Plus *F bs14b05.y1 AI945549 singleton CG10299|FBan0010299|CT28913|FBan0010299 99% Plus / Minus *F bs14b06.y1 AI945550 Contig465 CG4434|FBan0004434|CT14426|FBan0004434 99% Plus / Plus *F bs14b07.y1 AI945551 Contig430 none none *F bs14b08.y1 AI945552 Contig544 GH22881.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs14b09.y1 AI945553 Contig467 GH04277.5prime 99% Plus / Plus CG6541|FBan0006541|CT20379|FBan0006541 99% Plus / Plus *F bs14b10.y1 AI945554 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs14b11.y1 AI945555 Contig534 none none *F bs14b12.y1 AI945556 singleton GM05412.5prime 99% Plus / Plus CG12876|FBan0012876|CT32020|FBan0012876 100% Plus / Plus *F bs14c01.y1 AI945557 Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs14c02.y1 AI945558 singleton CG10950|FBan0010950|CT30671|FBan0010950 100% Plus / Plus *F bs14c03.y1 AI945559 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs14c04.y1 AI945560 Contig523 LD23336.3prime 100% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs14c05.y1 AI945561 singleton none none *F bs14c06.y1 AI945562 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs14c07.y1 AI945563 Contig544 none none *F bs14c08.y1 AI945564 Contig483 none none *F bs14c09.y1 AI945565 singleton CG5050|FBan0005050|CT16225|FBan0005050 97% Plus / Plus *F bs14c10.y1 AI945566 singleton CG17261|FBan0017261|CT35458|FBan0017261 100% Plus / Plus *F bs14c11.y1 AI945567 Contig407 CG3399|FBan0003399|CT11427|FBan0003399 99% Plus / Plus *F bs14c12.y1 AI945568 Contig231 CG18063|FBan0018063|CT40483|FBan0018063 97% Plus / Plus *F bs14d01.y1 AI945569 Contig155 CG4963|FBan0004963|CT15898|FBan0004963 98% Plus / Plus *F bs14d02.y1 AI945570 singleton CG14891|FBan0014891|CT34714|FBan0014891 99% Plus / Plus *F bs14d03.y1 AI945571 singleton LP11020.5prime 98% Plus / Plus CG18128|FBan0018128|CT40818|FBan0018128 98% Plus / Plus *F bs14d04.y1 AI945572 singleton CG12923|FBan0012923|CT32071|FBan0012923 100% Plus / Plus *F bs14d05.y1 AI945573 singleton GH20331.5prime 98% Plus / Plus CG14039|FBan0014039|CT33598|FBan0014039 98% Plus / Plus *F bs14d06.y1 AI945574 singleton LD31571.5prime 100% Plus / Plus CG7494|FBan0007494|CT23015|FBan0007494 100% Plus / Plus *F bs14d07.y1 AI945575 Contig511 GH22851.5prime 97% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs14d08.y1 AI945576 Contig520 CG2574|FBan0002574|CT8709|FBan0002574 100% Plus / Minus *F bs14d09.y1 AI945577 Contig384 CG3345|FBan0003345|CT36339|FBan0003345 99% Plus / Plus *F bs14d10.y1 AI945578 Contig152 GH05530.5prime 98% Plus / Plus CG17567|FBan0017567|CT35086|FBan0017567 98% Plus / Plus *F bs14d11.y1 AI945579 Contig544 none none *F bs14d12.y1 AI945580 Contig151 GH22577.3prime 98% Plus / Minus CG2727|FBan0002727|CT9269|FBan0002727 99% Plus / Plus *F bs14e01.y1 AI945581 Contig373 LD08335.5prime 100% Plus / Plus CG4390|FBan0004390|CT14318|FBan0004390 100% Plus / Plus *F bs14e02.y1 AI945582 Contig527 none none *F bs14e03.y1 AI945583 singleton LD09627.5prime 93% Plus / Plus CG10076|FBan0010076|CT28309|FBan0010076 93% Plus / Plus *F bs14e04.y1 AI945584 singleton GH03070.5prime 100% Plus / Plus CG11505|FBan0011505|CT36369|FBan0011505 100% Plus / Plus *F bs14e05.y1 AI945585 singleton GH03095.5prime 100% Plus / Plus CG9334|FBan0009334|CT3787|FBan0009334 100% Plus / Plus *F bs14e06.y1 AI945586 Contig502 GH01042.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs14e07.y1 AI945587 Contig150 CG5062|FBan0005062|CT16243|FBan0005062 99% Plus / Plus *F bs14e08.y1 AI945588 Contig444 LD35983.5prime 100% Plus / Plus CG2922|FBan0002922|CT7240|FBan0002922 100% Plus / Plus *F bs14e09.y1 AI945589 singleton none none *F bs14e10.y1 AI945590 Contig149 GM06116.5prime 100% Plus / Plus CG6822|FBan0006822|CT21141|FBan0006822 100% Plus / Plus *F bs14e11.y1 AI945591 singleton GH05625.5prime 100% Plus / Plus CG12749|FBan0012749|CT27250|FBan0012749 100% Plus / Plus *F bs14e12.y1 AI945592 Contig544 CG3124|FBan0003124|CT10470|FBan0003124 100% Plus / Plus *F bs14f01.y1 AI945593 Contig516 GH12755.5prime 98% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 98% Plus / Plus *F bs14f02.y1 AI945594 singleton LD07515.5prime 100% Plus / Plus CG8287|FBan0008287|CT24527|FBan0008287 100% Plus / Plus *F bs14f03.y1 AI945595 singleton CG17083|FBan0017083|CT37958|FBan0017083 98% Plus / Plus *F bs14f04.y1 AI945596 Contig190 GH17895.5prime 100% Plus / Plus CG3487|FBan0003487|CT11753|FBan0003487 100% Plus / Plus *F bs14f05.y1 AI945597 singleton LP09435.5prime 99% Plus / Plus CG9669|FBan0009669|CT27344|FBan0009669 99% Plus / Plus *F bs14f06.y1 AI945598 Contig520 none none *F bs14f07.y1 AI945599 Contig511 GH22851.5prime 98% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs14f08.y1 AI945600 singleton CG2871|FBan0002871|CT9824|FBan0002871 98% Plus / Plus *F bs14f09.y1 AI945601 singleton CG9173|FBan0009173|CT26226|FBan0009173 100% Plus / Plus *F bs14f10.y1 AI945602 Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs14f11.y1 AI945603 singleton CG10894|FBan0010894|CT30509|FBan0010894 99% Plus / Plus *F bs14f12.y1 AI945604 Contig359 CG12229|FBan0012229|CT12907|FBan0012229 98% Plus / Plus *F bs14g01.y1 AI945605 Contig544 GH19706.5prime 99% Plus / Plus CG4186|FBan0004186|CT13780|FBan0004186 99% Plus / Plus *F bs14g02.y1 AI945606 singleton CG17669|FBan0017669|CT34941|FBan0017669 98% Plus / Plus *F bs14g03.y1 AI945607 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 97% Plus / Plus *F bs14g04.y1 AI945608 singleton CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs14g05.y1 AI945609 Contig274 LD01152.5prime 95% Plus / Plus CG7701|FBan0007701|CT23463|FBan0007701 98% Plus / Plus *F bs14g06.y1 AI945610 Contig292 none none *F bs14g07.y1 AI945611 Contig297 GH08590.5prime 98% Plus / Plus CG3517|FBan0003517|CT11863|FBan0003517 98% Plus / Plus *F bs14g08.y1 AI945612 singleton CG17176|FBan0017176|CT38108|FBan0017176 99% Plus / Plus *F bs14g09.y1 AI945613 singleton CG10192|FBan0010192|CT28675|FBan0010192 99% Plus / Plus *F bs14g10.y1 AI945614 Contig355 GH23095.5prime 99% Plus / Plus CG8284|FBan0008284|CT24417|FBan0008284 99% Plus / Plus *F bs14g11.y1 AI945615 Contig468 GH13002.5prime 98% Plus / Plus CG13918|FBan0013918|CT33457|FBan0013918 98% Plus / Plus *F bs14g12.y1 AI945616 Contig544 CG4410|FBan0004410|CT4259|FBan0004410 100% Plus / Plus *F bs14h01.y1 AI945617 singleton GH18613.5prime 100% Plus / Plus CG18064|FBan0018064|CT40477|FBan0018064 100% Plus / Plus *F bs14h02.y1 AI945618 singleton GH20661.5prime 97% Plus / Plus CG4067|FBan0004067|CT13530|FBan0004067 100% Plus / Plus *F bs14h03.y1 AI945619 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 100% Plus / Plus *F bs14h04.y1 AI945620 singleton GH26474.5prime 100% Plus / Plus CG9848|FBan0009848|CT27792|FBan0009848 100% Plus / Plus *F bs14h05.y1 AI945621 Contig523 LD23336.3prime 98% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs14h06.y1 AI945622 Contig478 GH15369.5prime 100% Plus / Plus none none *F bs14h07.y1 AI945623 Contig471 none none *F bs14h08.y1 AI945624 Contig529 LP04643.5prime 98% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 96% Plus / Plus *F bs14h09.y1 AI945625 singleton LP04890.5prime 98% Plus / Plus CG11552|FBan0011552|CT20941|FBan0011552 98% Plus / Plus *F bs14h10.y1 AI945626 singleton GM02062.5prime 98% Plus / Plus CG3944|FBan0003944|CT10971|FBan0003944 100% Plus / Plus *F bs14h11.y1 AI945627 Contig370 LD41274.5prime 99% Plus / Plus CG4599|FBan0004599|CT14862|FBan0004599 99% Plus / Plus *F bs14h12.y1 AI945628 Contig542 none none *F bs15a02.y1 AI945629 Contig500 LD35407.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs15a03.y1 AI945630 Contig333 LD32507.3prime 98% Plus / Plus CG1913|FBan0001913|CT5908|FBan0001913 98% Plus / Minus *F bs15a04.y1 AI945631 singleton none none *F bs15a05.y1 AI945632 Contig365 SD10139.5prime 79% Plus / Plus CG5103|FBan0005103|CT16369|FBan0005103 99% Plus / Plus *F bs15a06.y1 AI945633 Contig361 GH11850.5prime 95% Plus / Plus CG4479|FBan0004479|CT14582|FBan0004479 99% Plus / Plus *F bs15a07.y1 AI945634 singleton none none *F bs15a08.y1 AI945635 singleton CG17510|FBan0017510|CT38721|FBan0017510 99% Plus / Minus *F bs15a09.y1 AI945636 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs15a10.y1 AI945637 Contig527 none none *F bs15a11.y1 pending singleton LD40095.5prime 97% Plus / Minus CG5938|FBan0005938|CT18660|FBan0005938 97% Plus / Minus *F bs15a12.y1 AI945638 singleton none none *F bs15b01.y1 AI945639 Contig206 CG6569|FBan0006569|CT20440|FBan0006569 98% Plus / Plus *F bs15b02.y1 AI945640 Contig478 GH11893.5prime 99% Plus / Plus none none *F bs15b03.y1 AI945641 singleton CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs15b04.y1 AI945642 singleton CG8958|FBan0008958|CT25736|FBan0008958 100% Plus / Plus *F bs15b05.y1 AI945643 singleton GH23979.5prime 100% Plus / Plus CG5654|FBan0005654|CT17850|FBan0005654 100% Plus / Plus *F bs15b06.y1 AI945644 singleton CG8204|FBan0008204|CT21907|FBan0008204 99% Plus / Plus *F bs15b07.y1 AI945645 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs15b08.y1 AI945646 Contig533 GH09435.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs15b09.y1 AI945647 Contig528 GH13107.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs15b10.y1 AI945648 Contig421 CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs15b11.y1 AI945649 Contig398 CG11588|FBan0011588|CT22407|FBan0011588 98% Plus / Plus *F bs15b12.y1 AI945650 Contig147 CG14668|FBan0014668|CT34450|FBan0014668 100% Plus / Plus *F bs15c01.y1 AI945651 singleton none none *F bs15c02.y1 AI945652 Contig145 CG7202|FBan0007202|CT22229|FBan0007202 100% Plus / Plus *F bs15c04.y1 AI945653 Contig475 LP02384.5prime 100% Plus / Plus CG7931|FBan0007931|CT6483|FBan0007931 98% Plus / Plus *F bs15c05.y1 AI945654 Contig483 CG15200|FBan0015200|CT35127|FBan0015200 100% Plus / Plus *F bs15c06.y1 AI945655 singleton LD07673.5prime 99% Plus / Plus CG1890|FBan0001890|CT5860|FBan0001890 99% Plus / Plus *F bs15c07.y1 AI945656 singleton LP05859.5prime 99% Plus / Plus CG12015|FBan0012015|CT1655|FBan0012015 99% Plus / Plus *F bs15c08.y1 AI945657 Contig340 GH01027.5prime 98% Plus / Minus CG10124|FBan0010124|CT28485|FBan0010124 99% Plus / Minus *F bs15c09.y1 AI945658 Contig331 CG17717|FBan0017717|CT39281|FBan0017717 99% Plus / Plus *F bs15c10.y1 AI945659 singleton SD10401.5prime 99% Plus / Plus CG12819|FBan0012819|CT31949|FBan0012819 99% Plus / Plus *F bs15c11.y1 AI945660 Contig536 LP09196.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs15c12.y1 AI945661 Contig478 SD07466.5prime 99% Plus / Plus none none *F bs15d03.y1 AI945662 singleton CG17154|FBan0017154|CT29096|FBan0017154 98% Plus / Plus *F bs15d07.y1 AI945663 Contig527 none none *F bs15d08.y1 AI945664 singleton CG13342|FBan0013342|CT32662|FBan0013342 98% Plus / Plus *F bs15d09.y1 pending Contig456 GH23516.5prime 98% Plus / Plus CG2149|FBan0002149|CT7028|FBan0002149 97% Plus / Plus *F bs15d10.y1 AI945665 singleton CG7676|FBan0007676|CT23159|FBan0007676 100% Plus / Plus *F bs15d11.y1 AI945666 Contig144 none none *F bs15d12.y1 AI945667 Contig365 CK01274.contig 99% Plus / Plus CG5103|FBan0005103|CT16369|FBan0005103 99% Plus / Plus *F bs15e01.y1 AI945668 singleton CG2854|FBan0002854|CT9762|FBan0002854 100% Plus / Plus *F bs15e02.y1 AI945669 Contig143 CG15425|FBan0015425|CT35487|FBan0015425 100% Plus / Plus *F bs15e03.y1 AI945670 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs15e04.y1 AI945671 Contig389 GH05725.5prime 92% Plus / Plus CG18078|FBan0018078|CT40551|FBan0018078 96% Plus / Plus *F bs15e05.y1 AI945672 singleton CG1345|FBan0001345|CT3038|FBan0001345 100% Plus / Plus *F bs15e07.y1 AI945673 singleton CG4719|FBan0004719|CT15154|FBan0004719 100% Plus / Minus *F bs15e08.y1 AI945674 Contig506 LP05425.5prime 99% Plus / Minus CG18396|FBan0018396|CT41800|FBan0018396 100% Plus / Plus *F bs15e09.y1 AI945675 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs15e10.y1 AI945676 Contig142 none none *F bs15e11.y1 AI945677 Contig533 GH21951.5prime 98% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs15e12.y1 AI945678 Contig141 none none *F bs15f01.y1 AI945679 Contig140 none none *F bs15f02.y1 AI945680 singleton CG6190|FBan0006190|CT19426|FBan0006190 100% Plus / Plus *F bs15f03.y1 AI945681 singleton CG11367|FBan0011367|CT26094|FBan0011367 99% Plus / Plus *F bs15f04.y1 AI945682 singleton LD27358.5prime 99% Plus / Plus CG10795|FBan0010795|CT30254|FBan0010795 100% Plus / Plus *F bs15f05.y1 AI945683 singleton LD37601.5prime 100% Plus / Minus CG4532|FBan0004532|CT14694|FBan0004532 100% Plus / Minus *F bs15f06.y1 AI945684 Contig468 GH13002.5prime 98% Plus / Plus CG13918|FBan0013918|CT33457|FBan0013918 98% Plus / Plus *F bs15f07.y1 AI945685 Contig139 CG8526|FBan0008526|CT24903|FBan0008526 100% Plus / Plus *F bs15f08.y1 AI945686 Contig452 CG3085|FBan0003085|CT10370|FBan0003085 100% Plus / Plus *F bs15f09.y1 AI945687 singleton GH04248.5prime 99% Plus / Plus CG7115|FBan0007115|CT21999|FBan0007115 100% Plus / Plus *F bs15f10.y1 AI945688 Contig537 GH07855.3prime 98% Plus / Minus CG8701|FBan0008701|CT7042|FBan0008701 99% Plus / Plus *F bs15f11.y1 AI945689 singleton LP01542.5prime 90% Plus / Minus none none *F bs15f12.y1 AI945690 Contig442 CG5024|FBan0005024|CT16147|FBan0005024 98% Plus / Plus *F bs15g01.y1 AI945691 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs15g02.y1 AI945692 Contig535 GH03745.5prime 100% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 100% Plus / Plus *F bs15g03.y1 AI945693 Contig168 CG4480|FBan0004480|CT14588|FBan0004480 97% Plus / Plus *F bs15g04.y1 AI945694 Contig243 CG11358|FBan0011358|CT31682|FBan0011358 99% Plus / Plus *F bs15g05.y1 AI945695 Contig420 GH12084.3prime 100% Plus / Minus CG6105|FBan0006105|CT19171|FBan0006105 100% Plus / Plus *F bs15g06.y1 pending Contig540 none none *F bs15g07.y1 AI945696 Contig433 SD09014.5prime 100% Plus / Plus CG4265|FBan0004265|CT10917|FBan0004265 99% Plus / Plus *F bs15g08.y1 AI945697 Contig515 GH06743.5prime 100% Plus / Minus none none *F bs15g09.y1 AI945698 singleton LP01985.5prime 100% Plus / Plus CG2931|FBan0002931|CT7840|FBan0002931 100% Plus / Plus *F bs15g10.y1 AI945699 singleton CG10718|FBan0010718|CT30035|FBan0010718 99% Plus / Plus *F bs15g11.y1 AI945700 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs15g12.y1 AI945701 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs15h02.y1 AI945702 singleton LD34139.5prime 100% Plus / Plus CG9745|FBan0009745|CT27541|FBan0009745 100% Plus / Plus *F bs15h03.y1 AI945703 Contig173 CK01602.contig 97% Plus / Plus CG8245|FBan0008245|CT2430|FBan0008245 100% Plus / Plus *F bs15h04.y1 AI945704 Contig316 CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs15h05.y1 AI945705 singleton LP08318.5prime 98% Plus / Plus CG13164|FBan0013164|CT32405|FBan0013164 94% Plus / Plus *F bs15h06.y1 AI945706 singleton CG13901|FBan0013901|CT33436|FBan0013901 96% Plus / Plus *F bs15h07.y1 AI945707 Contig228 SD05065.5prime 100% Plus / Plus CG8531|FBan0008531|CT24915|FBan0008531 100% Plus / Plus *F bs15h08.y1 AI945708 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs15h09.y1 AI945709 singleton none none *F bs15h10.y1 AI945710 Contig544 GH22881.5prime 98% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 98% Plus / Plus *F bs15h12.y1 AI945711 singleton CG15286|FBan0015286|CT35234|FBan0015286 99% Plus / Plus *F bs16a01.y1 pending Contig348 CG7349|FBan0007349|CT22629|FBan0007349 97% Plus / Plus *F bs16a02.y1 AI945712 singleton none none *F bs16a03.y1 AI945713 Contig138 GH01042.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs16a04.y1 AI945714 Contig528 CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs16a05.y1 AI945715 singleton GH04712.5prime 98% Plus / Plus CG6144|FBan0006144|CT19302|FBan0006144 96% Plus / Plus *F bs16a06.y1 AI945716 singleton GM07030.5prime 97% Plus / Plus CG7439|FBan0007439|CT22868|FBan0007439 100% Plus / Plus *F bs16a07.y1 pending singleton none none *F bs16a08.y1 AI945717 Contig395 LD07629.5prime 99% Plus / Plus CG8472|FBan0008472|CT24787|FBan0008472 99% Plus / Plus *F bs16a09.y1 AI945718 Contig486 CG7268|FBan0007268|CT22431|FBan0007268 98% Plus / Plus *F bs16a10.y1 AI945719 Contig480 GH15272.5prime 99% Plus / Plus CG1999|FBan0001999|CT6363|FBan0001999 100% Plus / Plus *F bs16a11.y1 AI945720 Contig305 CG12377|FBan0012377|CT25244|FBan0012377 98% Plus / Plus *F bs16a12.y1 AI945721 singleton CG6758|FBan0006758|CT20971|FBan0006758 99% Plus / Minus *F bs16b01.y1 pending singleton HL07724.5prime 99% Plus / Plus CG5206|FBan0005206|CT16645|FBan0005206 99% Plus / Plus *F bs16b02.y1 AI945722 singleton CG16721|FBan0016721|CT36019|FBan0016721 99% Plus / Plus *F bs16b03.y1 AI945723 Contig508 LD23817.3prime 98% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs16b04.y1 AI945724 singleton none none *F bs16b05.y1 AI945725 Contig495 GH19464.3prime 100% Plus / Plus CG9133|FBan0009133|CT10073|FBan0009133 100% Plus / Minus *F bs16b06.y1 AI945726 Contig495 CG10969|FBan0010969|CT30725|FBan0010969 100% Plus / Plus *F bs16b07.y1 AI945727 Contig137 LD10817.5prime 98% Plus / Plus CG7337|FBan0007337|CT10216|FBan0007337 99% Plus / Plus *F bs16b08.y1 AI945728 Contig519 GH07296.5prime 100% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs16b09.y1 pending Contig532 LP10167.3prime 98% Plus / Minus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs16b10.y1 AI945729 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs16b11.y1 AI945730 Contig538 GH10186.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs16b12.y1 AI945731 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs16c01.y1 AI945732 Contig136 CG15605|FBan0015605|CT35737|FBan0015605 99% Plus / Plus *F bs16c02.y1 AI945733 Contig135 CG7969|FBan0007969|CT23844|FBan0007969 98% Plus / Plus *F bs16c03.y1 AI945734 singleton CG11125|FBan0011125|CT8285|FBan0011125 100% Plus / Plus *F bs16c04.y1 AI945735 singleton CG3959|FBan0003959|CT13150|FBan0003959 98% Plus / Plus *F bs16c05.y1 AI945736 singleton GH10631.5prime 100% Plus / Plus CG5065|FBan0005065|CT16179|FBan0005065 98% Plus / Plus *F bs16c06.y1 AI945737 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs16c07.y1 AI945738 Contig335 GH04214.5prime 99% Plus / Plus CG4692|FBan0004692|CT15135|FBan0004692 99% Plus / Plus *F bs16c08.y1 AI945739 Contig133 GH26702.5prime 99% Plus / Plus CG4669|FBan0004669|CT15079|FBan0004669 100% Plus / Plus *F bs16c09.y1 AI945740 Contig462 none none *F bs16c10.y1 AI945741 Contig534 none none *F bs16c11.y1 AI945742 Contig258 CG9274|FBan0009274|CT26429|FBan0009274 100% Plus / Plus *F bs16c12.y1 pending singleton none none *F bs16d01.y1 AI945743 Contig534 none none *F bs16d02.y1 AI945744 singleton LD44353.5prime 99% Plus / Minus CG4913|FBan0004913|CT15784|FBan0004913 100% Plus / Plus *F bs16d03.y1 AI945745 Contig179 LD44893.5prime 100% Plus / Minus CG7414|FBan0007414|CT22817|FBan0007414 100% Plus / Plus *F bs16d04.y1 AI945746 singleton CG12126|FBan0012126|CT7604|FBan0012126 99% Plus / Plus *F bs16d05.y1 AI945747 singleton CG17789|FBan0017789|CT39404|FBan0017789 85% Plus / Plus *F bs16d06.y1 AI945748 Contig543 GH26043.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs16d08.y1 AI945749 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs16d09.y1 AI945750 Contig536 GH02210.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs16d10.y1 AI945751 Contig458 GH04159.5prime 99% Plus / Plus CG16758|FBan0016758|CT8044|FBan0016758 99% Plus / Plus *F bs16d11.y1 AI945752 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 97% Plus / Plus *F bs16d12.y1 AI945753 singleton GH28440.5prime 99% Plus / Plus CG5920|FBan0005920|CT18571|FBan0005920 99% Plus / Plus *F bs16e01.y1 pending singleton CG14084|FBan0014084|CT33673|FBan0014084 99% Plus / Plus *F bs16e02.y1 AI945754 Contig362 CG13747|FBan0013747|CT33223|FBan0013747 100% Plus / Plus *F bs16e03.y1 AI945755 singleton LP12114.5prime 99% Plus / Plus CG12304|FBan0012304|CT20383|FBan0012304 99% Plus / Plus *F bs16e04.y1 pending Contig130 GH05253.5prime 95% Plus / Plus none none *F bs16e05.y1 AI945756 Contig520 none none *F bs16e06.y1 AI945757 Contig529 LP04643.5prime 98% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 97% Plus / Plus *F bs16e07.y1 pending Contig256 CG6036|FBan0006036|CT18937|FBan0006036 98% Plus / Plus *F bs16e08.y1 AI945758 Contig129 CG8680|FBan0008680|CT13926|FBan0008680 98% Plus / Plus *F bs16e09.y1 AI945759 singleton none none *F bs16e10.y1 AI945760 Contig506 GH20038.5prime 99% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 99% Plus / Plus *F bs16e11.y1 AI945761 singleton GM04055.5prime 100% Plus / Plus CG8295|FBan0008295|CT21621|FBan0008295 99% Plus / Plus *F bs16e12.y1 AI945762 singleton GM09929.5prime 99% Plus / Plus CG3305|FBan0003305|CT11115|FBan0003305 100% Plus / Plus *F bs16f01.y1 pending Contig523 LD23336.3prime 98% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs16f02.y1 AI945763 Contig187 GH24303.5prime 100% Plus / Plus CG9983|FBan0009983|CT37548|FBan0009983 100% Plus / Plus *F bs16f03.y1 AI945764 singleton none none *F bs16f04.y1 AI945765 singleton LD28579.5prime 100% Plus / Minus CG7085|FBan0007085|CT21897|FBan0007085 100% Plus / Minus *F bs16f05.y1 AI945766 Contig539 GH27943.3prime 98% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs16f06.y1 AI945767 Contig543 GH26043.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs16f07.y1 AI945768 singleton HL04090.5prime 100% Plus / Plus CG17723|FBan0017723|CT39297|FBan0017723 100% Plus / Plus *F bs16f08.y1 AI945769 Contig516 CG3982|FBan0003982|CT13237|FBan0003982 98% Plus / Plus *F bs16f09.y1 AI945770 Contig128 CG11298|FBan0011298|CT31529|FBan0011298 99% Plus / Plus *F bs16f10.y1 AI945771 Contig338 GM01842.5prime 99% Plus / Plus CG10691|FBan0010691|CT29956|FBan0010691 98% Plus / Plus *F bs16f11.y1 AI945772 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs16f12.y1 AI945773 Contig527 none none *F bs16g01.y1 pending Contig513 LP02115.5prime 100% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs16g02.y1 AI945774 singleton CG15158|FBan0015158|CT35059|FBan0015158 100% Plus / Plus *F bs16g03.y1 AI945775 singleton LP04225.5prime 93% Plus / Plus CG3927|FBan0003927|CT8995|FBan0003927 100% Plus / Plus *F bs16g04.y1 AI945776 Contig528 GH06435.5prime 99% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs16g05.y1 AI945777 Contig493 CG14926|FBan0014926|CT34753|FBan0014926 99% Plus / Plus *F bs16g06.y1 AI945778 Contig538 GH15626.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs16g07.y1 AI945779 Contig438 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs16g08.y1 AI945780 singleton GH06241.5prime 100% Plus / Minus CG15101|FBan0015101|CT34976|FBan0015101 100% Plus / Minus *F bs16g09.y1 AI945781 Contig544 CG7311|FBan0007311|CT22241|FBan0007311 100% Plus / Plus *F bs16g10.y1 AI945782 Contig127 CG16894|FBan0016894|CT33392|FBan0016894 97% Plus / Plus *F bs16g11.y1 AI945783 Contig185 LD26964.5prime 99% Plus / Plus CG1837|FBan0001837|CT5608|FBan0001837 99% Plus / Plus *F bs16g12.y1 AI945784 singleton GH18528.5prime 100% Plus / Plus none none *F bs16h01.y1 pending Contig349 CG14355|FBan0014355|CT33990|FBan0014355 100% Plus / Plus *F bs16h02.y1 AI945785 Contig175 CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Minus *F bs16h03.y1 AI945786 singleton GH10408.3prime 99% Plus / Minus CG11444|FBan0011444|CT9463|FBan0011444 100% Plus / Plus *F bs16h04.y1 AI945787 Contig165 none none *F bs16h05.y1 AI945788 singleton CG14740|FBan0014740|CT34534|FBan0014740 100% Plus / Plus *F bs16h06.y1 AI945789 singleton CG18171|FBan0018171|CT41020|FBan0018171 99% Plus / Plus *F bs16h07.y1 AI945790 singleton none none *F bs16h08.y1 AI945791 singleton CK01021.contig 98% Plus / Plus CG8229|FBan0008229|CT8591|FBan0008229 100% Plus / Plus *F bs16h09.y1 AI945792 Contig543 GH27175.5prime 99% Plus / Plus CG4478|FBan0004478|CT14576|FBan0004478 99% Plus / Plus *F bs16h10.y1 AI945793 singleton CG12347|FBan0012347|CT23509|FBan0012347 100% Plus / Plus *F bs16h11.y1 AI945794 Contig411 CG11125|FBan0011125|CT8285|FBan0011125 100% Plus / Plus *F bs16h12.y1 AI945795 singleton CG3687|FBan0003687|CT12333|FBan0003687 98% Plus / Plus *F bs17a01.y1 AI945796 Contig474 GH09553.5prime 98% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 98% Plus / Plus *F bs17a02.y1 pending singleton CG13057|FBan0013057|CT32276|FBan0013057 99% Plus / Plus *F bs17a03.y1 AI945797 Contig407 CG3399|FBan0003399|CT11427|FBan0003399 99% Plus / Plus *F bs17a04.y1 AI945798 Contig123 CG9455|FBan0009455|CT11129|FBan0009455 100% Plus / Plus *F bs17a05.y1 AI945799 Contig122 CG7349|FBan0007349|CT22629|FBan0007349 96% Plus / Plus *F bs17a06.y1 AI945800 Contig461 GH13022.5prime 91% Plus / Plus CG6372|FBan0006372|CT19794|FBan0006372 91% Plus / Plus *F bs17a07.y1 AI945801 singleton GH14158.5prime 96% Plus / Plus CG1009|FBan0001009|CT1016|FBan0001009 97% Plus / Plus *F bs17a08.y1 AI945802 singleton LP04452.5prime 100% Plus / Plus CG9920|FBan0009920|CT27932|FBan0009920 100% Plus / Plus *F bs17a09.y1 AI945803 Contig536 LP09196.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs17a10.y1 AI945804 Contig522 LP09246.5prime 99% Plus / Plus none none *F bs17a12.y1 AI945805 Contig502 GH26978.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs17b01.y1 AI945806 Contig242 LP02587.5prime 99% Plus / Plus CG1548|FBan0001548|CT3996|FBan0001548 99% Plus / Plus *F bs17b02.y1 AI945807 Contig528 GH06435.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs17b03.y1 AI945808 singleton CG10421|FBan0010421|CT29262|FBan0010421 99% Plus / Plus *F bs17b04.y1 AI945809 singleton LP12332.5prime 99% Plus / Plus CG6038|FBan0006038|CT18941|FBan0006038 99% Plus / Plus *F bs17b05.y1 AI945810 Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs17b06.y1 AI945811 singleton CG8914|FBan0008914|CT25590|FBan0008914 98% Plus / Plus *F bs17b07.y1 AI945812 Contig510 GH13495.3prime 98% Plus / Minus CG10513|FBan0010513|CT29500|FBan0010513 98% Plus / Plus *F bs17b08.y1 AI945813 Contig444 GM08970.5prime 100% Plus / Plus CG2922|FBan0002922|CT7240|FBan0002922 100% Plus / Plus *F bs17b09.y1 AI945814 Contig121 CG9624|FBan0009624|CT27222|FBan0009624 100% Plus / Plus *F bs17b10.y1 AI945815 Contig150 CG5062|FBan0005062|CT16243|FBan0005062 99% Plus / Plus *F bs17b11.y1 AI945816 singleton none none *F bs17b12.y1 AI945817 Contig543 GH18676.3prime 98% Plus / Minus CG18184|FBan0018184|CT41084|FBan0018184 96% Plus / Plus *F bs17c01.y1 AI945818 singleton CG3354|FBan0003354|CT11217|FBan0003354 91% Plus / Plus *F bs17c02.y1 AI945819 singleton CG16792|FBan0016792|CT25454|FBan0016792 100% Plus / Plus *F bs17c03.y1 AI945820 Contig481 CG5051|FBan0005051|CT16124|FBan0005051 100% Plus / Plus *F bs17c04.y1 AI945821 Contig497 GH27078.5prime 97% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 99% Plus / Plus *F bs17c05.y1 AI945822 singleton GH05928.5prime 99% Plus / Plus CG3800|FBan0003800|CT12687|FBan0003800 99% Plus / Plus *F bs17c06.y1 AI945823 singleton none none *F bs17c07.y1 AI945824 Contig334 CG14801|FBan0014801|CT34614|FBan0014801 100% Plus / Plus *F bs17c08.y1 AI945825 Contig480 GH15272.5prime 99% Plus / Plus CG1999|FBan0001999|CT6363|FBan0001999 100% Plus / Plus *F bs17c09.y1 AI945826 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs17c10.y1 AI945827 Contig441 GH26265.5prime 100% Plus / Plus none none *F bs17c11.y1 AI945828 Contig478 LD10585.5prime 100% Plus / Plus none none *F bs17c12.y1 AI945829 Contig120 none none *F bs17d01.y1 AI945830 Contig538 GH15626.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs17d02.y1 AI945831 singleton none none *F bs17d04.y1 AI945832 Contig144 GH28709.5prime 100% Plus / Plus CG18427|FBan0018427|CT30112|FBan0018427 100% Plus / Plus *F bs17d05.y1 AI945833 singleton none none *F bs17d06.y1 AI945834 singleton LP08020.3prime 99% Plus / Minus CG4264|FBan0004264|CT13958|FBan0004264 99% Plus / Plus *F bs17d07.y1 AI945835 Contig154 SD06593.5prime 100% Plus / Plus CG1728|FBan0001728|CT4964|FBan0001728 100% Plus / Plus *F bs17d08.y1 AI945836 singleton GM13131.3prime 100% Plus / Minus CG11143|FBan0011143|CT31151|FBan0011143 100% Plus / Plus *F bs17d09.y1 AI945837 singleton GH07453.3prime 98% Plus / Minus CG4559|FBan0004559|CT14570|FBan0004559 99% Plus / Plus *F bs17d10.y1 pending Contig120 CG3222|FBan0003222|CT10697|FBan0003222 99% Plus / Minus *F bs17d11.y1 AI945838 singleton CG15569|FBan0015569|CT35685|FBan0015569 99% Plus / Plus *F bs17d12.y1 AI945839 singleton LD39010.5prime 100% Plus / Plus CG5275|FBan0005275|CT16835|FBan0005275 100% Plus / Plus *F bs17e01.y1 AI945840 singleton CG2649|FBan0002649|CT8971|FBan0002649 100% Plus / Plus *F bs17e02.y1 AI945841 Contig161 LD40711.5prime 99% Plus / Plus CG11562|FBan0011562|CT33146|FBan0011562 99% Plus / Plus *F bs17e03.y1 AI945842 singleton CG8356|FBan0008356|CT37647|FBan0008356 99% Plus / Plus *F bs17e04.y1 AI945843 singleton CG7213|FBan0007213|CT22247|FBan0007213 100% Plus / Plus *F bs17e05.y1 AI945844 Contig502 GH01042.5prime 98% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 98% Plus / Plus *F bs17e06.y1 AI945845 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 96% Plus / Plus *F bs17e07.y1 AI945846 Contig332 CG6497|FBan0006497|CT20241|FBan0006497 100% Plus / Plus *F bs17e08.y1 AI945847 singleton LD03175.5prime 99% Plus / Plus CG3268|FBan0003268|CT9209|FBan0003268 100% Plus / Plus *F bs17e09.y1 AI945848 singleton GH10637.5prime 95% Plus / Plus CG9156|FBan0009156|CT26196|FBan0009156 96% Plus / Plus *F bs17e10.y1 AI945849 Contig544 GH03736.5prime 99% Plus / Plus CG3124|FBan0003124|CT10470|FBan0003124 99% Plus / Plus *F bs17e11.y1 AI945850 singleton LP12021.5prime 99% Plus / Plus CG10306|FBan0010306|CT28939|FBan0010306 99% Plus / Plus *F bs17e12.y1 AI945851 singleton LP07643.5prime 98% Plus / Plus CG4944|FBan0004944|CT15683|FBan0004944 98% Plus / Plus *F bs17f01.y1 AI945852 singleton GH03217.5prime 98% Plus / Plus none none *F bs17f02.y1 AI945853 singleton LP06636.5prime 81% Plus / Plus CG8937|FBan0008937|CT25664|FBan0008937 98% Plus / Plus *F bs17f03.y1 AI945854 singleton CG7477|FBan0007477|CT22979|FBan0007477 99% Plus / Plus *F bs17f04.y1 AI945855 singleton LD22503.3prime 99% Plus / Minus CG2331|FBan0002331|CT7776|FBan0002331 99% Plus / Plus *F bs17f05.y1 AI945856 Contig416 LD17341.5prime 100% Plus / Plus none none *F bs17f06.y1 AI945857 Contig140 none none *F bs17f07.y1 AI945858 singleton GH09088.5prime 100% Plus / Plus CG6304|FBan0006304|CT19728|FBan0006304 100% Plus / Plus *F bs17f09.y1 AI945859 singleton GH18851.5prime 99% Plus / Plus CG8417|FBan0008417|CT24707|FBan0008417 99% Plus / Plus *F bs17f10.y1 pending Contig525 none none *F bs17f11.y1 AI945860 Contig543 GH18035.5prime 99% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 100% Plus / Plus *F bs17f12.y1 AI945861 singleton GH23290.5prime 99% Plus / Plus CG15693|FBan0015693|CT35894|FBan0015693 99% Plus / Plus *F bs17g01.y1 AI945862 Contig153 CG8277|FBan0008277|CT24507|FBan0008277 99% Plus / Plus *F bs17g02.y1 AI945863 singleton LD41235.5prime 97% Plus / Plus CG17033|FBan0017033|CT32297|FBan0017033 97% Plus / Plus *F bs17g03.y1 AI945864 Contig527 none none *F bs17g04.y1 AI945865 Contig514 GH24336.5prime 100% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 100% Plus / Plus *F bs17g05.y1 AI945866 singleton none none *F bs17g06.y1 AI945867 Contig496 CG7441|FBan0007441|CT22887|FBan0007441 100% Plus / Plus *F bs17g07.y1 AI945868 Contig238 CG8368|FBan0008368|CT24647|FBan0008368 99% Plus / Plus *F bs17g08.y1 AI945869 singleton LD02743.5prime 98% Plus / Minus CG2093|FBan0002093|CT6820|FBan0002093 99% Plus / Minus *F bs17g09.y1 AI945870 singleton CG5103|FBan0005103|CT16369|FBan0005103 98% Plus / Plus *F bs17g10.y1 AI945871 singleton CG3763|FBan0003763|CT12582|FBan0003763 98% Plus / Plus *F bs17g11.y1 AI945872 Contig515 GH16259.5prime 100% Plus / Minus none none *F bs17g12.y1 AI945873 Contig522 LP09246.5prime 96% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 99% Plus / Plus *F bs17h01.y1 AI945874 Contig533 GH06096.5prime 100% Plus / Plus none none *F bs17h02.y1 AI945875 singleton LD44211.5prime 97% Plus / Plus CG2028|FBan0002028|CT30917|FBan0002028 97% Plus / Plus *F bs17h03.y1 AI945876 singleton CG7453|FBan0007453|CT22925|FBan0007453 99% Plus / Plus *F bs17h04.y1 pending Contig330 LP07429.5prime 98% Plus / Plus CG3222|FBan0003222|CT10697|FBan0003222 98% Plus / Plus *F bs17h05.y1 AI945877 Contig217 CG13164|FBan0013164|CT32405|FBan0013164 98% Plus / Plus *F bs17h06.y1 AI945878 Contig489 LP11879.5prime 100% Plus / Plus CG5045|FBan0005045|CT16189|FBan0005045 100% Plus / Plus *F bs17h07.y1 AI945879 singleton CG13394|FBan0013394|CT32740|FBan0013394 96% Plus / Plus *F bs17h08.y1 AI945880 Contig487 none none *F bs17h09.y1 AI945881 Contig543 GH09553.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs17h10.y1 AI945882 singleton CG11369|FBan0011369|CT31720|FBan0011369 100% Plus / Plus *F bs17h11.y1 AI945883 singleton GH20113.5prime 98% Plus / Plus none none *F bs17h12.y1 AI945884 singleton none none *F bs18a01.y1 AI945885 Contig295 GH18016.5prime 99% Plus / Plus CG3492|FBan0003492|CT11747|FBan0003492 99% Plus / Plus *F bs18a02.y1 AI945886 Contig463 CG10690|FBan0010690|CT29964|FBan0010690 100% Plus / Minus *F bs18a03.y1 AI945887 Contig500 LD35407.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs18a04.y1 AI945888 Contig438 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs18a05.y1 AI945889 Contig115 CG1338|FBan0001338|CT2966|FBan0001338 98% Plus / Plus *F bs18a06.y1 AI945890 Contig454 CG2164|FBan0002164|CT7072|FBan0002164 100% Plus / Plus *F bs18a07.y1 AI945891 singleton LD36950.3prime 98% Plus / Plus CG18214|FBan0018214|CT41210|FBan0018214 99% Plus / Minus *F bs18a08.y1 AI945892 singleton GH25258.5prime 100% Plus / Plus CG11396|FBan0011396|CT31819|FBan0011396 100% Plus / Plus *F bs18a09.y1 AI945893 Contig543 GH09553.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs18a10.y1 AI945894 singleton LD24770.5prime 99% Plus / Plus CG11652|FBan0011652|CT36663|FBan0011652 99% Plus / Plus *F bs18a11.y1 AI945895 Contig114 LP09831.5prime 100% Plus / Plus CG14996|FBan0014996|CT34849|FBan0014996 100% Plus / Plus *F bs18a12.y1 AI945896 singleton none none *F bs18b01.y1 AI945897 singleton GH25459.5prime 99% Plus / Plus CG10849|FBan0010849|CT30379|FBan0010849 100% Plus / Plus *F bs18b02.y1 AI945898 Contig482 CG18449|FBan0018449|CT42026|FBan0018449 99% Plus / Plus *F bs18b03.y1 AI945899 singleton CG4093|FBan0004093|CT13586|FBan0004093 97% Plus / Plus *F bs18b04.y1 AI945900 Contig536 GH02210.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs18b05.y1 AI945901 Contig534 none none *F bs18b07.y1 AI945902 singleton CG7264|FBan0007264|CT22423|FBan0007264 99% Plus / Plus *F bs18b08.y1 AI945903 Contig148 CG4631|FBan0004631|CT14966|FBan0004631 99% Plus / Plus *F bs18b09.y1 AI945904 singleton none none *F bs18b10.y1 AI945905 singleton GM02135.5prime 97% Plus / Plus CG3994|FBan0003994|CT13257|FBan0003994 97% Plus / Plus *F bs18b11.y1 AI945906 singleton CG12630|FBan0012630|CT35156|FBan0012630 100% Plus / Plus *F bs18b12.y1 AI945907 Contig540 none none *F bs18c01.y1 AI945908 Contig410 CG17946|FBan0017946|CT39984|FBan0017946 100% Plus / Plus *F bs18c02.y1 AI945909 Contig129 CG8680|FBan0008680|CT13926|FBan0008680 98% Plus / Plus *F bs18c03.y1 AI945910 singleton CG7756|FBan0007756|CT23565|FBan0007756 99% Plus / Plus *F bs18c04.y1 AI945911 Contig538 GH09790.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs18c05.y1 AI945912 Contig108 none none *F bs18c06.y1 AI945913 Contig107 GH25462.5prime 97% Plus / Plus none none *F bs18c07.y1 AI945914 Contig343 CG18370|FBan0018370|CT41755|FBan0018370 98% Plus / Plus *F bs18c08.y1 AI945915 Contig106 CG6603|FBan0006603|CT39144|FBan0006603 99% Plus / Plus *F bs18c09.y1 AI945916 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs18c10.y1 AI945917 singleton GH24215.5prime 99% Plus / Plus CG8367|FBan0008367|CT24629|FBan0008367 99% Plus / Plus *F bs18c11.y1 AI945918 Contig521 GH27033.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs18c12.y1 AI945919 Contig189 GH22024.5prime 99% Plus / Plus CG8209|FBan0008209|CT24423|FBan0008209 99% Plus / Plus *F bs18d01.y1 pending Contig522 LP09246.5prime 98% Plus / Plus none none *F bs18d02.y1 AI945920 Contig519 GH24914.5prime 100% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs18d03.y1 AI945921 Contig404 GH06830.5prime 99% Plus / Plus CG14718|FBan0014718|CT34509|FBan0014718 99% Plus / Plus *F bs18d04.y1 AI945922 Contig105 LD19790.5prime 99% Plus / Plus CG12051|FBan0012051|CT3675|FBan0012051 100% Plus / Plus *F bs18d05.y1 AI945923 Contig351 GH16413.5prime 98% Plus / Plus CG4767|FBan0004767|CT15347|FBan0004767 99% Plus / Plus *F bs18d06.y1 AI945924 Contig513 LP02115.5prime 99% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs18d07.y1 AI945925 Contig103 LP02209.3prime 99% Plus / Minus CG13993|FBan0013993|CT33549|FBan0013993 100% Plus / Plus *F bs18d08.y1 AI945926 Contig493 CG14926|FBan0014926|CT34753|FBan0014926 98% Plus / Minus *F bs18d09.y1 AI945927 singleton CK01731.5prime 99% Plus / Plus CG8368|FBan0008368|CT24647|FBan0008368 99% Plus / Plus *F bs18d11.y1 AI945928 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs18d12.y1 AI945929 singleton none none *F bs18e01.y1 AI945930 Contig307 LP07781.5prime 97% Plus / Plus CG17819|FBan0017819|CT39518|FBan0017819 97% Plus / Plus *F bs18e02.y1 AI945931 Contig477 none none *F bs18e03.y1 AI945932 singleton none none *F bs18e04.y1 AI945933 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs18e05.y1 AI945934 Contig504 CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs18e06.y1 AI945935 Contig435 LP05191.5prime 100% Plus / Plus CG12562|FBan0012562|CT34305|FBan0012562 100% Plus / Plus *F bs18e07.y1 AI945936 Contig328 GH19655.5prime 99% Plus / Plus CG15208|FBan0015208|CT35139|FBan0015208 99% Plus / Plus *F bs18e08.y1 AI945937 singleton CG18193|FBan0018193|CT41122|FBan0018193 99% Plus / Plus *F bs18e09.y1 AI945938 Contig406 GH05530.5prime 99% Plus / Plus CG17567|FBan0017567|CT35086|FBan0017567 99% Plus / Plus *F bs18e10.y1 AI945939 singleton GM01553.5prime 97% Plus / Plus CG12384|FBan0012384|CT25962|FBan0012384 99% Plus / Plus *F bs18e11.y1 pending singleton CG8657|FBan0008657|CT25080|FBan0008657 100% Plus / Plus *F bs18e12.y1 AI945940 singleton CG17177|FBan0017177|CT38112|FBan0017177 98% Plus / Plus *F bs18f01.y1 AI945941 Contig521 GH27033.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs18f03.y1 AI945942 Contig126 CG4706|FBan0004706|CT15177|FBan0004706 100% Plus / Minus *F bs18f04.y1 AI945943 Contig462 CG18427|FBan0018427|CT30112|FBan0018427 100% Plus / Plus *F bs18f05.y1 AI945944 Contig540 none none *F bs18f06.y1 AI945945 Contig101 CG8851|FBan0008851|CT9281|FBan0008851 98% Plus / Plus *F bs18f07.y1 AI945946 Contig124 LD24809.5prime 99% Plus / Plus CG1416|FBan0001416|CT2833|FBan0001416 99% Plus / Plus *F bs18f08.y1 AI945947 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs18f09.y1 AI945948 Contig488 LP11873.5prime 99% Plus / Plus none none *F bs18f10.y1 AI945949 Contig119 none none *F bs18f11.y1 AI945950 Contig503 GH11521.5prime 96% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 96% Plus / Plus *F bs18f12.y1 AI945951 Contig327 LD27049.5prime 98% Plus / Plus CG9828|FBan0009828|CT27734|FBan0009828 99% Plus / Plus *F bs18g01.y1 pending Contig521 GH27033.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs18g03.y1 AI945952 Contig126 CG4706|FBan0004706|CT15177|FBan0004706 100% Plus / Minus *F bs18g04.y1 AI945953 Contig462 CG18427|FBan0018427|CT30112|FBan0018427 100% Plus / Plus *F bs18g05.y1 AI945954 Contig540 none none *F bs18g06.y1 AI945955 Contig101 CG8851|FBan0008851|CT9281|FBan0008851 98% Plus / Plus *F bs18g07.y1 AI945956 Contig124 LD24809.5prime 99% Plus / Plus CG1416|FBan0001416|CT2833|FBan0001416 99% Plus / Plus *F bs18g08.y1 AI945957 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs18g09.y1 AI945958 Contig488 GH20014.5prime 99% Plus / Plus none none *F bs18g10.y1 AI945959 Contig119 none none *F bs18g12.y1 AI945960 Contig327 LD27049.5prime 98% Plus / Plus CG9828|FBan0009828|CT27734|FBan0009828 100% Plus / Plus *F bs18h01.y1 AI945961 singleton LD05795.5prime 98% Plus / Minus CG2183|FBan0002183|CT7140|FBan0002183 99% Plus / Minus *F bs18h02.y1 AI945962 singleton CK00535.5prime 98% Plus / Plus CG7555|FBan0007555|CT23113|FBan0007555 99% Plus / Plus *F bs18h03.y1 AI945963 Contig098 CG18491|FBan0018491|CT42152|FBan0018491 100% Plus / Plus *F bs18h04.y1 AI945964 singleton LD08235.5prime 98% Plus / Plus CG7590|FBan0007590|CT23193|FBan0007590 99% Plus / Plus *F bs18h05.y1 AI945965 Contig519 GH05452.5prime 99% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs18h06.y1 AI945966 Contig536 GH14152.5prime 100% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 99% Plus / Plus *F bs18h07.y1 AI945967 Contig446 LP09632.5prime 99% Plus / Plus CG3691|FBan0003691|CT12381|FBan0003691 99% Plus / Plus *F bs18h08.y1 AI945968 singleton none none *F bs18h09.y1 AI945969 singleton CG6143|FBan0006143|CT41974|FBan0006143 99% Plus / Plus *F bs18h10.y1 AI945970 singleton CG11037|FBan0011037|CT30893|FBan0011037 99% Plus / Plus *F bs18h11.y1 AI945971 Contig482 CG18449|FBan0018449|CT42026|FBan0018449 99% Plus / Plus *F bs18h12.y1 pending Contig437 GH15530.5prime 99% Plus / Plus CG1014|FBan0001014|CT1028|FBan0001014 99% Plus / Plus *F bs19a01.y1 pending singleton CG1868|FBan0001868|CT5774|FBan0001868 99% Plus / Plus *F bs19a02.y1 AI945972 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs19a03.y1 AI945973 Contig507 LD23670.3prime 99% Plus / Minus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs19a04.y1 AI945974 Contig291 GH23468.3prime 100% Plus / Minus CG3184|FBan0003184|CT10667|FBan0003184 99% Plus / Minus *F bs19a05.y1 AI945975 singleton none none *F bs19a06.y1 pending Contig097 CG5261|FBan0005261|CT42460|FBan0005261 100% Plus / Plus *F bs19a07.y1 pending Contig521 none none *F bs19a08.y1 AI945976 Contig326 LP11903.5prime 99% Plus / Plus CG1534|FBan0001534|CT3947|FBan0001534 99% Plus / Plus *F bs19a09.y1 AI945977 singleton LD36566.5prime 99% Plus / Plus CG1532|FBan0001532|CT3959|FBan0001532 99% Plus / Plus *F bs19a10.y1 AI945978 singleton LP09222.5prime 100% Plus / Plus CG10230|FBan0010230|CT28749|FBan0010230 100% Plus / Plus *F bs19a11.y1 AI945979 Contig215 none none *F bs19a12.y1 AI945980 Contig543 CG17934|FBan0017934|CT39960|FBan0017934 100% Plus / Plus *F bs19b03.y1 AI945981 singleton CK01660.3prime 96% Plus / Plus CG9166|FBan0009166|CT8251|FBan0009166 99% Plus / Minus *F bs19b04.y1 pending Contig454 CG2164|FBan0002164|CT7072|FBan0002164 97% Plus / Plus *F bs19b05.y1 AI945982 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 100% Plus / Plus *F bs19b07.y1 AI945983 Contig117 GM02731.5prime 98% Plus / Minus none none *F bs19b08.y1 AI945984 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs19b09.y1 AI945985 singleton CG17003|FBan0017003|CT35311|FBan0017003 100% Plus / Plus *F bs19b10.y1 AI945986 singleton LD15036.5prime 100% Plus / Plus CG2163|FBan0002163|CT7068|FBan0002163 100% Plus / Plus *F bs19b11.y1 AI945987 Contig500 LD35407.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs19b12.y1 AI945988 singleton CG18369|FBan0018369|CT41749|FBan0018369 100% Plus / Plus *F bs19c02.y1 AI945989 Contig421 CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs19c03.y1 AI945990 singleton CG5755|FBan0005755|CT18089|FBan0005755 98% Plus / Plus *F bs19c05.y1 AI945991 singleton GH08122.3prime 100% Plus / Minus CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs19c07.y1 AI945992 singleton GH28564.5prime 99% Plus / Plus CG12101|FBan0012101|CT6068|FBan0012101 99% Plus / Plus *F bs19c08.y1 AI945993 singleton none none *F bs19c09.y1 AI945994 singleton LD26545.5prime 98% Plus / Minus CG2107|FBan0002107|CT1795|FBan0002107 99% Plus / Plus *F bs19c10.y1 AI945995 Contig518 GH22658.5prime 97% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs19c11.y1 AI945996 singleton CG11109|FBan0011109|CT31087|FBan0011109 99% Plus / Minus *F bs19c12.y1 AI945997 Contig349 CG3711|FBan0003711|CT12449|FBan0003711 100% Plus / Plus *F bs19d03.y1 AI945998 Contig492 CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs19d05.y1 AI945999 Contig544 GH25863.5prime 98% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 98% Plus / Plus *F bs19d06.y1 AI946000 Contig485 none none *F bs19d07.y1 AI946001 Contig465 CG4434|FBan0004434|CT14426|FBan0004434 100% Plus / Plus *F bs19d08.y1 AI946002 singleton LP01220.3prime 99% Plus / Plus none none *F bs19d09.y1 AI946003 Contig543 CK01680.3prime 99% Plus / Minus none none *F bs19d10.y1 AI946004 singleton none none *F bs19d11.y1 AI946005 Contig473 GH11857.5prime 100% Plus / Plus CG14290|FBan0014290|CT33919|FBan0014290 98% Plus / Plus *F bs19e04.y1 AI946006 singleton none none *F bs19e05.y1 AI946007 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 100% Plus / Plus *F bs19e06.y1 pending Contig544 GH22513.5prime 97% Plus / Plus none none *F bs19e07.y1 AI946008 singleton CG12677|FBan0012677|CT35495|FBan0012677 100% Plus / Plus *F bs19e08.y1 AI946009 singleton none none *F bs19e09.y1 AI946010 singleton CG2336|FBan0002336|CT7778|FBan0002336 100% Plus / Plus *F bs19e10.y1 AI946011 singleton GH15083.5prime 99% Plus / Minus none none *F bs19e11.y1 AI946012 Contig463 CG10690|FBan0010690|CT29964|FBan0010690 100% Plus / Minus *F bs19f02.y1 pending singleton LD25413.5prime 95% Plus / Plus CG5604|FBan0005604|CT17724|FBan0005604 99% Plus / Plus *F bs19f03.y1 AI946013 Contig377 LP04712.5prime 99% Plus / Plus none none *F bs19f04.y1 pending singleton none none *F bs19f05.y1 AI946014 Contig475 LP02384.5prime 100% Plus / Plus CG7931|FBan0007931|CT6483|FBan0007931 98% Plus / Plus *F bs19f06.y1 AI946015 Contig094 CG14402|FBan0014402|CT34051|FBan0014402 100% Plus / Plus *F bs19f07.y1 AI946016 Contig356 none none *F bs19f08.y1 AI946017 Contig364 GM01939.5prime 99% Plus / Plus CG9992|FBan0009992|CT28165|FBan0009992 99% Plus / Plus *F bs19f09.y1 AI946018 singleton CG12161|FBan0012161|CT8835|FBan0012161 100% Plus / Plus *F bs19f10.y1 AI946019 singleton LD45331.5prime 100% Plus / Plus CG14648|FBan0014648|CT34420|FBan0014648 100% Plus / Plus *F bs19f11.y1 AI946020 Contig543 CG4750|FBan0004750|CT15217|FBan0004750 99% Plus / Plus *F bs19g01.y1 AI946021 Contig110 CG3575|FBan0003575|CT12015|FBan0003575 99% Plus / Plus *F bs19g02.y1 AI946022 Contig226 CG7712|FBan0007712|CT23487|FBan0007712 98% Plus / Plus *F bs19g03.y1 AI946023 Contig447 GH12486.5prime 95% Plus / Plus CG4375|FBan0004375|CT14270|FBan0004375 95% Plus / Plus *F bs19g04.y1 pending Contig122 CG7349|FBan0007349|CT22629|FBan0007349 97% Plus / Plus *F bs19g05.y1 pending Contig188 CG2076|FBan0002076|CT6605|FBan0002076 100% Plus / Plus *F bs19g06.y1 AI946024 singleton LP05325.5prime 100% Plus / Plus CG15445|FBan0015445|CT35509|FBan0015445 100% Plus / Plus *F bs19g07.y1 pending Contig336 none none *F bs19g08.y1 AI946025 singleton none none *F bs19g09.y1 AI946026 Contig396 CG11393|FBan0011393|CT31813|FBan0011393 100% Plus / Plus *F bs19g10.y1 AI946027 singleton CG11806|FBan0011806|CT36921|FBan0011806 100% Plus / Plus *F bs19g11.y1 AI946028 Contig091 LP05168.3prime 99% Plus / Minus none none *F bs19g12.y1 AI946029 singleton CG13211|FBan0013211|CT41165|FBan0013211 99% Plus / Plus *F bs19h02.y1 pending Contig544 GH10815.5prime 98% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 98% Plus / Plus *F bs19h03.y1 AI946030 singleton CG11254|FBan0011254|CT31389|FBan0011254 98% Plus / Plus *F bs19h04.y1 AI946031 singleton CG5509|FBan0005509|CT17232|FBan0005509 100% Plus / Plus *F bs19h05.y1 AI946032 Contig508 LD23817.3prime 100% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs19h06.y1 AI946033 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs19h07.y1 AI946034 singleton LD32290.5prime 98% Plus / Plus CG8931|FBan0008931|CT25646|FBan0008931 99% Plus / Plus *F bs19h08.y1 AI946035 Contig139 CG8526|FBan0008526|CT24903|FBan0008526 100% Plus / Plus *F bs19h09.y1 AI946036 singleton LD19312.5prime 98% Plus / Plus CG8443|FBan0008443|CT19935|FBan0008443 100% Plus / Plus *F bs19h10.y1 AI946037 Contig114 GH28730.5prime 100% Plus / Plus CG14996|FBan0014996|CT34849|FBan0014996 100% Plus / Plus *F bs19h11.y1 AI946038 Contig378 CG1137|FBan0001137|CT1904|FBan0001137 98% Plus / Plus *F bs19h12.y1 AI946039 singleton GH19825.5prime 97% Plus / Plus CG9748|FBan0009748|CT27543|FBan0009748 97% Plus / Plus *F bs20a01.y1 AI946040 Contig323 LD32260.5prime 98% Plus / Plus CG9288|FBan0009288|CT26469|FBan0009288 100% Plus / Plus *F bs20a03.y1 AI946041 Contig434 CG7164|FBan0007164|CT22119|FBan0007164 100% Plus / Plus *F bs20a04.y1 AI946042 Contig360 GH23889.5prime 98% Plus / Plus CG5106|FBan0005106|CT15693|FBan0005106 99% Plus / Plus *F bs20a05.y1 AI946043 singleton CG12635|FBan0012635|CT42136|FBan0012635 99% Plus / Minus *F bs20a06.y1 pending singleton SD05682.5prime 100% Plus / Minus CG8286|FBan0008286|CT24529|FBan0008286 100% Plus / Minus *F bs20a07.y1 AI946044 Contig276 LP06848.5prime 100% Plus / Plus CG17462|FBan0017462|CT31210|FBan0017462 100% Plus / Plus *F bs20a09.y1 AI946045 Contig511 GH22851.5prime 98% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs20a10.y1 pending Contig427 CG18131|FBan0018131|CT40834|FBan0018131 99% Plus / Plus *F bs20a11.y1 AI946046 singleton CG3552|FBan0003552|CT11946|FBan0003552 100% Plus / Plus *F bs20a12.y1 AI946047 Contig412 GH14048.5prime 100% Plus / Plus CG7131|FBan0007131|CT22049|FBan0007131 100% Plus / Plus *F bs20b01.y1 AI946048 singleton CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs20b02.y1 AI946049 Contig539 GH27943.3prime 99% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs20b03.y1 AI946050 Contig197 GH06775.5prime 100% Plus / Plus CG7251|FBan0007251|CT22371|FBan0007251 100% Plus / Plus *F bs20b04.y1 pending Contig090 LD31958.5prime 99% Plus / Minus CG17654|FBan0017654|CT32526|FBan0017654 100% Plus / Plus *F bs20b06.y1 pending singleton GH13434.5prime 91% Plus / Plus CG10869|FBan0010869|CT30429|FBan0010869 92% Plus / Plus *F bs20b08.y1 AI946051 Contig505 CG11781|FBan0011781|CT33060|FBan0011781 100% Plus / Plus *F bs20b09.y1 pending Contig491 CG6332|FBan0006332|CT19816|FBan0006332 99% Plus / Plus *F bs20b10.y1 AI946052 singleton none none *F bs20b11.y1 AI946053 Contig470 none none *F bs20c01.y1 pending Contig486 CG7268|FBan0007268|CT22431|FBan0007268 97% Plus / Plus *F bs20c02.y1 pending singleton CG6652|FBan0006652|CT20666|FBan0006652 97% Plus / Plus *F bs20c04.y1 AI946054 Contig496 CG7441|FBan0007441|CT22887|FBan0007441 100% Plus / Plus *F bs20c05.y1 pending singleton LD11149.3prime 97% Plus / Minus CG7892|FBan0007892|CT23293|FBan0007892 99% Plus / Plus *F bs20c06.y1 pending Contig460 GH10940.5prime 97% Plus / Minus CG18662|FBan0018662|CT42585|FBan0018662 97% Plus / Plus *F bs20c07.y1 AI946055 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs20c08.y1 AI946056 Contig334 CG14801|FBan0014801|CT34614|FBan0014801 99% Plus / Plus *F bs20c09.y1 AI946057 Contig438 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs20c10.y1 AI946058 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs20c11.y1 AI946059 Contig515 HL03427.5prime 100% Plus / Plus none none *F bs20d01.y1 AI946060 singleton GH09659.5prime 99% Plus / Plus CG4710|FBan0004710|CT15107|FBan0004710 99% Plus / Plus *F bs20d02.y1 AI946061 singleton CG14712|FBan0014712|CT34503|FBan0014712 100% Plus / Plus *F bs20d03.y1 pending Contig531 GH04958.5prime 99% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 98% Plus / Plus *F bs20d04.y1 pending Contig479 none none *F bs20d05.y1 AI946062 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 99% Plus / Plus *F bs20d06.y1 AI946063 singleton none none *F bs20d07.y1 AI946064 Contig100 GH27735.5prime 99% Plus / Minus CG10219|FBan0010219|CT28735|FBan0010219 99% Plus / Minus *F bs20d08.y1 pending Contig534 none none *F bs20d09.y1 AI946065 singleton LD22345.5prime 99% Plus / Minus CG6845|FBan0006845|CT21203|FBan0006845 100% Plus / Minus *F bs20d10.y1 AI946066 Contig535 GH03745.5prime 100% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 100% Plus / Plus *F bs20d11.y1 AI946067 Contig432 LD26681.5prime 100% Plus / Plus CG8938|FBan0008938|CT25660|FBan0008938 100% Plus / Plus *F bs20d12.y1 pending Contig391 CG4270|FBan0004270|CT37757|FBan0004270 99% Plus / Plus *F bs20e01.y1 pending Contig463 CG10690|FBan0010690|CT29964|FBan0010690 97% Plus / Minus *F bs20e02.y1 pending Contig093 LP11475.5prime 99% Plus / Plus CG15109|FBan0015109|CT34984|FBan0015109 99% Plus / Plus *F bs20e03.y1 AI946068 singleton none none *F bs20e04.y1 AI946069 singleton CG5569|FBan0005569|CT17608|FBan0005569 99% Plus / Plus *F bs20e05.y1 pending Contig164 GH09392.5prime 98% Plus / Plus CG10589|FBan0010589|CT29692|FBan0010589 98% Plus / Plus *F bs20e06.y1 AI946070 singleton CG15817|FBan0015817|CT37070|FBan0015817 98% Plus / Minus *F bs20e07.y1 AI946071 Contig496 CK00537.5prime 100% Plus / Plus CG7441|FBan0007441|CT22887|FBan0007441 100% Plus / Plus *F bs20e08.y1 AI946072 singleton CG4477|FBan0004477|CT14578|FBan0004477 99% Plus / Plus *F bs20e09.y1 pending Contig412 GH14048.5prime 100% Plus / Plus CG7131|FBan0007131|CT22049|FBan0007131 100% Plus / Plus *F bs20e10.y1 AI946073 Contig095 GH23890.5prime 100% Plus / Plus CG8251|FBan0008251|CT2835|FBan0008251 100% Plus / Plus *F bs20e11.y1 AI946074 singleton LD31680.5prime 99% Plus / Plus CG6677|FBan0006677|CT20746|FBan0006677 100% Plus / Plus *F bs20e12.y1 AI946075 singleton CG5075|FBan0005075|CT16217|FBan0005075 100% Plus / Plus *F bs20f01.y1 AI946076 Contig087 CG15461|FBan0015461|CT35530|FBan0015461 100% Plus / Plus *F bs20f03.y1 AI946077 Contig190 GH17895.5prime 100% Plus / Plus CG3487|FBan0003487|CT11753|FBan0003487 100% Plus / Plus *F bs20f04.y1 AI946078 Contig086 LD34931.5prime 100% Plus / Plus CG5113|FBan0005113|CT16407|FBan0005113 100% Plus / Plus *F bs20f05.y1 AI946079 Contig445 LP10010.5prime 100% Plus / Minus CG1288|FBan0001288|CT2110|FBan0001288 99% Plus / Minus *F bs20f06.y1 AI946080 Contig427 CG18131|FBan0018131|CT40834|FBan0018131 99% Plus / Plus *F bs20f07.y1 AI946081 singleton GH08383.5prime 100% Plus / Plus CG6255|FBan0006255|CT19574|FBan0006255 100% Plus / Plus *F bs20f08.y1 AI946082 singleton CG11362|FBan0011362|CT31698|FBan0011362 97% Plus / Plus *F bs20f09.y1 AI946083 singleton LP02810.5prime 99% Plus / Plus CG11635|FBan0011635|CT34556|FBan0011635 99% Plus / Plus *F bs20f10.y1 AI946084 Contig408 GH17939.5prime 97% Plus / Plus CG4983|FBan0004983|CT15981|FBan0004983 97% Plus / Plus *F bs20f11.y1 pending singleton LD36456.5prime 99% Plus / Plus CG17369|FBan0017369|CT42376|FBan0017369 99% Plus / Plus *F bs20f12.y1 AI946085 Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs20g01.y1 AI946086 Contig085 LP10814.5prime 90% Plus / Plus none none *F bs20g02.y1 AI946087 Contig479 none none *F bs20g03.y1 AI946088 singleton HL05817.5prime 99% Plus / Plus CG3305|FBan0003305|CT11115|FBan0003305 100% Plus / Plus *F bs20g04.y1 AI946089 singleton CG13923|FBan0013923|CT33462|FBan0013923 99% Plus / Minus *F bs20g05.y1 AI946090 Contig496 CK01295.3prime 96% Plus / Plus CG7441|FBan0007441|CT22887|FBan0007441 97% Plus / Minus *F bs20g06.y1 pending Contig506 LP10995.5prime 99% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 99% Plus / Plus *F bs20g07.y1 AI946091 singleton LD01649.3prime 99% Plus / Minus CG6693|FBan0006693|CT20796|FBan0006693 99% Plus / Plus *F bs20g08.y1 pending Contig446 GH19547.3prime 99% Plus / Plus CG3691|FBan0003691|CT12381|FBan0003691 99% Plus / Plus *F bs20g09.y1 AI946092 singleton none none *F bs20g10.y1 AI946093 Contig484 GH03227.5prime 100% Plus / Plus CG5538|FBan0005538|CT17502|FBan0005538 100% Plus / Plus *F bs20g11.y1 AI946094 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs20g12.y1 AI946095 singleton SD06977.5prime 100% Plus / Plus CG15438|FBan0015438|CT35502|FBan0015438 100% Plus / Plus *F bs20h01.y1 AI946096 singleton GH01662.3prime 99% Plus / Minus CG3982|FBan0003982|CT13237|FBan0003982 98% Plus / Plus *F bs20h02.y1 AI946097 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 100% Plus / Plus *F bs20h03.y1 AI946098 Contig348 CG7349|FBan0007349|CT22629|FBan0007349 99% Plus / Plus *F bs20h04.y1 AI946099 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs20h06.y1 AI946100 singleton CG11893|FBan0011893|CT33087|FBan0011893 99% Plus / Plus *F bs20h07.y1 AI946101 Contig331 CG17717|FBan0017717|CT39281|FBan0017717 99% Plus / Plus *F bs20h08.y1 AI946102 Contig084 GH23779.5prime 99% Plus / Plus CG1837|FBan0001837|CT5608|FBan0001837 99% Plus / Plus *F bs20h09.y1 AI946103 Contig334 SD04906.5prime 100% Plus / Plus CG14800|FBan0014800|CT34613|FBan0014800 100% Plus / Plus *F bs20h10.y1 AI946104 Contig508 LD23817.3prime 100% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs20h11.y1 AI946105 Contig497 GH14434.5prime 97% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 99% Plus / Plus *F bs20h12.y1 AI946106 singleton GH26112.5prime 100% Plus / Plus none none *F bs21a01.y1 AI946107 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs21a02.y1 AI946108 Contig534 none none *F bs21a03.y1 pending Contig540 none none *F bs21a04.y1 AI946109 Contig281 LD30350.5prime 99% Plus / Plus CG5802|FBan0005802|CT18200|FBan0005802 99% Plus / Plus *F bs21a05.y1 AI946110 Contig087 CG15461|FBan0015461|CT35530|FBan0015461 100% Plus / Plus *F bs21a06.y1 AI946111 Contig327 LD13258.5prime 99% Plus / Plus CG9828|FBan0009828|CT27734|FBan0009828 99% Plus / Plus *F bs21a07.y1 pending Contig127 CG13184|FBan0013184|CT32425|FBan0013184 100% Plus / Plus *F bs21a08.y1 AI946112 singleton LP03146.5prime 94% Plus / Plus CG13330|FBan0013330|CT32649|FBan0013330 96% Plus / Plus *F bs21a09.y1 AI946113 singleton CG13111|FBan0013111|CT32348|FBan0013111 100% Plus / Minus *F bs21a10.y1 AI946114 singleton SD05444.5prime 99% Plus / Plus CG17767|FBan0017767|CT33834|FBan0017767 99% Plus / Plus *F bs21a11.y1 AI946115 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs21b01.y1 AI946116 Contig436 CG2574|FBan0002574|CT8709|FBan0002574 100% Plus / Plus *F bs21b02.y1 AI946117 Contig083 GH05694.5prime 100% Plus / Plus CG1979|FBan0001979|CT6207|FBan0001979 100% Plus / Plus *F bs21b03.y1 AI946118 Contig241 LP05266.5prime 99% Plus / Plus CG14717|FBan0014717|CT34508|FBan0014717 99% Plus / Plus *F bs21b04.y1 AI946119 Contig266 LP03412.5prime 99% Plus / Plus CG8979|FBan0008979|CT25810|FBan0008979 100% Plus / Plus *F bs21b05.y1 AI946120 singleton none none *F bs21b06.y1 AI946121 singleton LP06514.5prime 97% Plus / Plus CG5280|FBan0005280|CT16863|FBan0005280 97% Plus / Plus *F bs21b07.y1 pending Contig473 GH11857.5prime 99% Plus / Plus CG14290|FBan0014290|CT33919|FBan0014290 91% Plus / Plus *F bs21b08.y1 AI946122 Contig207 CG13477|FBan0013477|CT32842|FBan0013477 99% Plus / Plus *F bs21b09.y1 AI946123 singleton none none *F bs21b10.y1 AI946124 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs21b11.y1 AI946125 Contig508 LD23817.3prime 100% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs21b12.y1 AI946126 singleton none none *F bs21c01.y1 AI946127 Contig502 GH01042.5prime 98% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 98% Plus / Plus *F bs21c02.y1 AI946128 Contig525 none none *F bs21c04.y1 AI946129 Contig477 none none *F bs21c05.y1 AI946130 Contig089 none none *F bs21c06.y1 AI946131 Contig478 GH14022.5prime 99% Plus / Plus CG8732|FBan0008732|CT25221|FBan0008732 99% Plus / Plus *F bs21c07.y1 AI946132 Contig332 CG6497|FBan0006497|CT20241|FBan0006497 99% Plus / Plus *F bs21c08.y1 AI946133 Contig458 GH08656.5prime 99% Plus / Plus CG16758|FBan0016758|CT8044|FBan0016758 99% Plus / Plus *F bs21c09.y1 AI946134 Contig433 GH25235.5prime 99% Plus / Plus CG4265|FBan0004265|CT10917|FBan0004265 99% Plus / Plus *F bs21c10.y1 AI946135 singleton CG9254|FBan0009254|CT26022|FBan0009254 99% Plus / Plus *F bs21c11.y1 AI946136 Contig476 LP11595.5prime 98% Plus / Plus CG3752|FBan0003752|CT12541|FBan0003752 99% Plus / Plus *F bs21c12.y1 AI946137 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs21d01.y1 AI946138 Contig544 LP02148.5prime 100% Plus / Plus CG7048|FBan0007048|CT21799|FBan0007048 100% Plus / Plus *F bs21d02.y1 AI946139 singleton CG9008|FBan0009008|CT25890|FBan0009008 100% Plus / Plus *F bs21d03.y1 AI946140 singleton CG11106|FBan0011106|CT31085|FBan0011106 100% Plus / Plus *F bs21d04.y1 AI946141 singleton LP09854.5prime 98% Plus / Plus CG10177|FBan0010177|CT28615|FBan0010177 98% Plus / Plus *F bs21d05.y1 AI946142 singleton GH13901.3prime 99% Plus / Minus CG9010|FBan0009010|CT25878|FBan0009010 99% Plus / Plus *F bs21d06.y1 AI946143 Contig468 GH13002.5prime 99% Plus / Plus CG13918|FBan0013918|CT33457|FBan0013918 98% Plus / Plus *F bs21d07.y1 AI946144 Contig537 GH19032.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs21d08.y1 AI946145 Contig482 CG18449|FBan0018449|CT42026|FBan0018449 99% Plus / Plus *F bs21d09.y1 AI946146 Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs21d11.y1 AI946147 singleton GH27120.5prime 99% Plus / Plus CG17765|FBan0017765|CT7494|FBan0017765 99% Plus / Plus *F bs21e01.y1 AI946148 Contig544 LP06279.5prime 100% Plus / Minus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Minus *F bs21e02.y1 AI946149 Contig536 GH19031.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs21e03.y1 AI946150 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs21e04.y1 AI946151 singleton LD24839.3prime 99% Plus / Plus CG6794|FBan0006794|CT20770|FBan0006794 100% Plus / Plus *F bs21e06.y1 AI946152 Contig425 CG17344|FBan0017344|CT35082|FBan0017344 99% Plus / Plus *F bs21e07.y1 AI946153 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs21e08.y1 AI946154 Contig099 CG5906|FBan0005906|CT18549|FBan0005906 98% Plus / Plus *F bs21e09.y1 AI946155 Contig208 none none *F bs21e11.y1 AI946156 Contig491 GH07879.5prime 99% Plus / Plus CG6332|FBan0006332|CT19816|FBan0006332 99% Plus / Plus *F bs21e12.y1 AI946157 Contig424 GH24085.5prime 100% Plus / Minus CG1394|FBan0001394|CT3204|FBan0001394 100% Plus / Minus *F bs21f01.y1 AI946158 Contig544 LP05965.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs21f02.y1 AI946159 singleton none none *F bs21f03.y1 AI946160 Contig423 GM02127.5prime 99% Plus / Plus CG1383|FBan0001383|CT3168|FBan0001383 100% Plus / Plus *F bs21f04.y1 AI946161 Contig109 LD15307.5prime 99% Plus / Plus CG8104|FBan0008104|CT24240|FBan0008104 100% Plus / Plus *F bs21f05.y1 AI946162 singleton CG11567|FBan0011567|CT26884|FBan0011567 99% Plus / Plus *F bs21f06.y1 AI946163 Contig532 GH16267.5prime 97% Plus / Plus CG17349|FBan0017349|CT32310|FBan0017349 97% Plus / Plus *F bs21f07.y1 AI946164 singleton GH10507.3prime 96% Plus / Minus CG17101|FBan0017101|CT37996|FBan0017101 99% Plus / Plus *F bs21f08.y1 AI946165 Contig451 GH08251.5prime 100% Plus / Plus CG18628|FBan0018628|CT41623|FBan0018628 100% Plus / Plus *F bs21f09.y1 AI946166 singleton none none *F bs21f10.y1 AI946167 Contig462 CG18427|FBan0018427|CT30112|FBan0018427 98% Plus / Plus *F bs21f11.y1 AI946168 singleton GM02757.5prime 99% Plus / Plus CG5374|FBan0005374|CT17016|FBan0005374 100% Plus / Plus *F bs21f12.y1 AI946169 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 100% Plus / Plus *F bs21g01.y1 AI946170 Contig532 LP11026.5prime 100% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs21g02.y1 AI946171 Contig531 GH04958.5prime 99% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs21g03.y1 AI946172 singleton LD25913.5prime 98% Plus / Plus CG9670|FBan0009670|CT27346|FBan0009670 98% Plus / Plus *F bs21g04.y1 AI946173 singleton GH27943.3prime 98% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs21g05.y1 AI946174 Contig479 CG5735|FBan0005735|CT18021|FBan0005735 100% Plus / Plus *F bs21g06.y1 AI946175 Contig102 CG17030|FBan0017030|CT33160|FBan0017030 99% Plus / Plus *F bs21g07.y1 AI946176 singleton none none *F bs21g08.y1 AI946177 singleton none none *F bs21g09.y1 AI946178 singleton CG8598|FBan0008598|CT14502|FBan0008598 98% Plus / Plus *F bs21g10.y1 AI946179 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs21g11.y1 AI946180 singleton GH10585.5prime 99% Plus / Plus CG8974|FBan0008974|CT25788|FBan0008974 99% Plus / Plus *F bs21h01.y1 AI946181 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs21h02.y1 AI946182 Contig425 CG17344|FBan0017344|CT35082|FBan0017344 99% Plus / Plus *F bs21h03.y1 AI946183 Contig529 LP04643.5prime 99% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs21h04.y1 AI946184 Contig121 CG9624|FBan0009624|CT27222|FBan0009624 99% Plus / Plus *F bs21h05.y1 AI946185 singleton CG16940|FBan0016940|CT21258|FBan0016940 99% Plus / Plus *F bs21h06.y1 AI946186 singleton LD37086.5prime 100% Plus / Plus CG6963|FBan0006963|CT21414|FBan0006963 100% Plus / Plus *F bs21h07.y1 AI946187 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs21h08.y1 AI946188 Contig418 LP08248.5prime 100% Plus / Plus CG10578|FBan0010578|CT29670|FBan0010578 100% Plus / Plus *F bs21h09.y1 AI946189 singleton CG6333|FBan0006333|CT19808|FBan0006333 99% Plus / Plus *F bs21h10.y1 AI946190 singleton GH14519.5prime 86% Plus / Plus CG7756|FBan0007756|CT23565|FBan0007756 99% Plus / Plus *F bs21h11.y1 AI946191 singleton GH18968.5prime 100% Plus / Plus CG8597|FBan0008597|CT15293|FBan0008597 100% Plus / Plus *F bs21h12.y1 AI946192 Contig416 LD17341.5prime 99% Plus / Minus CG6319|FBan0006319|CT19690|FBan0006319 100% Plus / Plus *F bs22a01.y1 AI946193 Contig262 LD14382.5prime 98% Plus / Plus CG18638|FBan0018638|CT42589|FBan0018638 99% Plus / Plus *F bs22a02.y1 AI946194 Contig534 none none *F bs22a03.y1 AI946195 Contig098 CG18491|FBan0018491|CT42152|FBan0018491 100% Plus / Plus *F bs22a04.y1 AI946196 singleton LD20645.3prime 100% Plus / Minus CG11181|FBan0011181|CT31079|FBan0011181 99% Plus / Plus *F bs22a05.y1 AI946197 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs22a06.y1 AI946198 singleton CG7069|FBan0007069|CT21853|FBan0007069 97% Plus / Plus *F bs22a07.y1 AI946199 Contig506 GH20038.5prime 99% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 99% Plus / Plus *F bs22a08.y1 AI946200 singleton CG11692|FBan0011692|CT36727|FBan0011692 99% Plus / Minus *F bs22a09.y1 AI946201 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 99% Plus / Plus *F bs22a10.y1 AI946202 singleton CG15676|FBan0015676|CT35861|FBan0015676 99% Plus / Plus *F bs22a11.y1 AI946203 Contig529 none none *F bs22a12.y1 AI946204 singleton LP11508.5prime 100% Plus / Plus CG4553|FBan0004553|CT14748|FBan0004553 100% Plus / Plus *F bs22b02.y1 AI946205 Contig134 GH06747.5prime 99% Plus / Plus CG1821|FBan0001821|CT5526|FBan0001821 99% Plus / Plus *F bs22b03.y1 AI946206 singleton none none *F bs22b04.y1 AI946207 Contig422 CG9323|FBan0009323|CT3608|FBan0009323 99% Plus / Plus *F bs22b05.y1 AI946208 Contig542 GH26674.5prime 99% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs22b06.y1 AI946209 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs22b07.y1 AI946210 Contig475 LP02384.5prime 100% Plus / Plus CG7931|FBan0007931|CT6483|FBan0007931 100% Plus / Plus *F bs22b08.y1 AI946211 Contig280 CG14738|FBan0014738|CT34531|FBan0014738 99% Plus / Plus *F bs22b09.y1 pending singleton none none *F bs22b10.y1 AI946212 Contig466 CG2267|FBan0002267|CT7048|FBan0002267 99% Plus / Plus *F bs22b11.y1 AI946213 Contig479 GH28038.5prime 100% Plus / Plus CG5735|FBan0005735|CT18021|FBan0005735 96% Plus / Plus *F bs22b12.y1 AI946214 Contig543 CG11428|FBan0011428|CT31897|FBan0011428 98% Plus / Plus *F bs22c01.y1 AI946215 singleton GH09955.5prime 99% Plus / Plus none none *F bs22c02.y1 AI946216 Contig415 GH05991.5prime 98% Plus / Plus CG9106|FBan0009106|CT26128|FBan0009106 98% Plus / Plus *F bs22c03.y1 AI946217 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs22c04.y1 AI946218 Contig536 GH14152.5prime 100% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 99% Plus / Plus *F bs22c05.y1 AI946219 Contig414 CG12853|FBan0012853|CT31992|FBan0012853 100% Plus / Plus *F bs22c06.y1 AI946220 Contig543 none none *F bs22c07.y1 AI946221 singleton GH21645.5prime 99% Plus / Plus CG10014|FBan0010014|CT28209|FBan0010014 99% Plus / Plus *F bs22c08.y1 AI946222 Contig453 CG9314|FBan0009314|CT26521|FBan0009314 98% Plus / Plus *F bs22c09.y1 AI946223 singleton LD09631.5prime 98% Plus / Plus CG17153|FBan0017153|CT38094|FBan0017153 99% Plus / Plus *F bs22c10.y1 AI946224 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs22c11.y1 AI946225 Contig337 GH07823.3prime 99% Plus / Minus CG8430|FBan0008430|CT18945|FBan0008430 99% Plus / Plus *F bs22c12.y1 AI946226 singleton none none *F bs22d01.y1 AI946227 Contig402 GM01663.5prime 100% Plus / Plus CG3265|FBan0003265|CT37737|FBan0003265 99% Plus / Plus *F bs22d02.y1 AI946228 Contig542 none none *F bs22d03.y1 AI946229 Contig391 CG4270|FBan0004270|CT37757|FBan0004270 99% Plus / Plus *F bs22d04.y1 AI946230 singleton GM09065.3prime 95% Plus / Minus CG9075|FBan0009075|CT26044|FBan0009075 99% Plus / Plus *F bs22d05.y1 AI946231 Contig541 CG4691|FBan0004691|CT15105|FBan0004691 100% Plus / Plus *F bs22d06.y1 AI946232 Contig090 LD15491.3prime 99% Plus / Plus none none *F bs22d07.y1 AI946233 Contig529 LD24460.3prime 100% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 100% Plus / Plus *F bs22d08.y1 AI946234 Contig429 CG6380|FBan0006380|CT19908|FBan0006380 99% Plus / Plus *F bs22d09.y1 AI946235 Contig498 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT38382|FBan0018461 97% Plus / Plus *F bs22d10.y1 pending singleton CG10714|FBan0010714|CT30029|FBan0010714 98% Plus / Plus *F bs22d11.y1 AI946236 Contig538 GH09790.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs22d12.y1 AI946237 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs22e01.y1 AI946238 Contig508 LD23817.3prime 98% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs22e02.y1 AI946239 singleton CK02472.3prime 98% Plus / Plus CG14709|FBan0014709|CT34500|FBan0014709 100% Plus / Minus *F bs22e03.y1 AI946240 Contig522 LP09246.5prime 99% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 100% Plus / Plus *F bs22e04.y1 AI946241 Contig106 LP01630.5prime 100% Plus / Plus CG6603|FBan0006603|CT39144|FBan0006603 99% Plus / Plus *F bs22e06.y1 AI946242 Contig543 none none *F bs22e07.y1 AI946243 singleton CK01713.5prime 97% Plus / Minus none none *F bs22e08.y1 AI946244 singleton CG14009|FBan0014009|CT33566|FBan0014009 99% Plus / Plus *F bs22e09.y1 AI946245 Contig464 CG14297|FBan0014297|CT33927|FBan0014297 99% Plus / Plus *F bs22e10.y1 AI946246 Contig543 none none *F bs22e11.y1 AI946247 Contig329 GH02218.3prime 98% Plus / Minus CG10749|FBan0010749|CT30132|FBan0010749 98% Plus / Plus *F bs22e12.y1 AI946248 singleton SD05031.5prime 100% Plus / Plus CG3186|FBan0003186|CT10685|FBan0003186 99% Plus / Plus *F bs22f01.y1 AI946249 Contig461 LP08687.5prime 95% Plus / Plus CG6372|FBan0006372|CT19794|FBan0006372 95% Plus / Plus *F bs22f02.y1 AI946250 Contig373 GH03475.5prime 100% Plus / Plus CG4390|FBan0004390|CT14318|FBan0004390 100% Plus / Plus *F bs22f03.y1 AI946251 Contig450 GH23475.5prime 100% Plus / Plus none none *F bs22f04.y1 AI946252 Contig539 GH14654.5prime 99% Plus / Plus CG5164|FBan0005164|CT16545|FBan0005164 99% Plus / Plus *F bs22f06.y1 AI946253 Contig544 CG8278|FBan0008278|CT7966|FBan0008278 99% Plus / Plus *F bs22f07.y1 AI946254 singleton HL02010.5prime 100% Plus / Minus none none *F bs22f08.y1 AI946255 singleton GH18257.5prime 99% Plus / Plus CG4268|FBan0004268|CT13850|FBan0004268 100% Plus / Plus *F bs22f09.y1 AI946256 Contig543 LD11680.5prime 97% Plus / Plus CG4400|FBan0004400|CT4159|FBan0004400 100% Plus / Plus *F bs22f10.y1 AI946257 singleton CG5021|FBan0005021|CT16118|FBan0005021 100% Plus / Plus *F bs22f12.y1 AI946258 Contig533 GH21951.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs22g01.y1 AI946259 singleton LD46049.5prime 99% Plus / Plus CG8749|FBan0008749|CT14488|FBan0008749 99% Plus / Plus *F bs22g02.y1 AI946260 Contig539 none none *F bs22g03.y1 AI946261 Contig094 CG14402|FBan0014402|CT34051|FBan0014402 100% Plus / Plus *F bs22g04.y1 AI946262 Contig147 CG14668|FBan0014668|CT34450|FBan0014668 97% Plus / Plus *F bs22g05.y1 AI946263 Contig405 CG10164|FBan0010164|CT28591|FBan0010164 99% Plus / Plus *F bs22g06.y1 AI946264 Contig330 LP07429.5prime 98% Plus / Plus CG3222|FBan0003222|CT10697|FBan0003222 98% Plus / Plus *F bs22g07.y1 AI946265 Contig125 LD28892.5prime 99% Plus / Plus CG17791|FBan0017791|CT39414|FBan0017791 100% Plus / Plus *F bs22g08.y1 AI946266 Contig497 GH14434.5prime 97% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 97% Plus / Plus *F bs22g09.y1 AI946267 singleton LD21122.5prime 92% Plus / Plus CG4211|FBan0004211|CT13866|FBan0004211 94% Plus / Plus *F bs22g10.y1 AI946268 singleton GH15774.3prime 100% Plus / Minus CG1193|FBan0001193|CT2194|FBan0001193 100% Plus / Plus *F bs22g11.y1 AI946269 singleton CG9404|FBan0009404|CT26687|FBan0009404 99% Plus / Plus *F bs22g12.y1 AI946270 Contig473 GH11857.5prime 100% Plus / Plus CG14290|FBan0014290|CT33919|FBan0014290 100% Plus / Plus *F bs22h01.y1 AI946271 Contig542 GH26674.5prime 98% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs22h02.y1 AI946272 Contig500 GM08717.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs22h03.y1 AI946273 Contig245 CK00367.5prime 99% Plus / Plus CG15111|FBan0015111|CT34989|FBan0015111 99% Plus / Plus *F bs22h04.y1 AI946274 singleton GM03174.5prime 99% Plus / Plus CG11246|FBan0011246|CT31387|FBan0011246 98% Plus / Plus *F bs22h05.y1 AI946275 Contig519 LD23817.3prime 100% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs22h06.y1 AI946276 Contig539 GH27943.3prime 98% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs22h07.y1 AI946277 Contig516 GH12755.5prime 100% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 100% Plus / Plus *F bs22h08.y1 AI946278 singleton GH13843.5prime 99% Plus / Plus CG9470|FBan0009470|CT26820|FBan0009470 99% Plus / Plus *F bs22h09.y1 AI946279 Contig177 GH20729.5prime 100% Plus / Plus CG3967|FBan0003967|CT39420|FBan0003967 99% Plus / Plus *F bs22h10.y1 pending singleton GH09210.5prime 100% Plus / Plus CG8746|FBan0008746|CT8615|FBan0008746 99% Plus / Plus *F bs22h11.y1 AI946280 singleton CG5603|FBan0005603|CT17708|FBan0005603 100% Plus / Plus *F bs22h12.y1 AI946281 singleton CG11251|FBan0011251|CT31407|FBan0011251 98% Plus / Plus *F bs23a02.y1 AI946282 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs23a04.y1 AI946283 singleton LD34133.5prime 99% Plus / Plus CG17743|FBan0017743|CT39329|FBan0017743 100% Plus / Plus *F bs23a06.y1 AI946284 singleton LD34613.5prime 100% Plus / Plus CG3269|FBan0003269|CT9197|FBan0003269 100% Plus / Plus *F bs23a07.y1 AI946285 Contig469 CG8732|FBan0008732|CT25221|FBan0008732 100% Plus / Minus *F bs23a10.y1 AI946286 Contig271 GH07855.3prime 98% Plus / Minus CG8701|FBan0008701|CT7042|FBan0008701 100% Plus / Plus *F bs23a11.y1 pending Contig349 CG14355|FBan0014355|CT33990|FBan0014355 100% Plus / Plus *F bs23a12.y1 AI946287 singleton SD09954.5prime 99% Plus / Plus CG9735|FBan0009735|CT27510|FBan0009735 99% Plus / Plus *F bs23b01.y1 AI946288 singleton LD26923.3prime 100% Plus / Minus none none *F bs23b02.y1 AI946289 singleton none none *F bs23b03.y1 pending singleton GH08959.5prime 96% Plus / Plus CG8552|FBan0008552|CT24897|FBan0008552 95% Plus / Plus *F bs23b04.y1 pending Contig325 CG11327|FBan0011327|CT31615|FBan0011327 97% Plus / Plus *F bs23b05.y1 AI946290 singleton CG9484|FBan0009484|CT26854|FBan0009484 100% Plus / Plus *F bs23b06.y1 AI946291 Contig325 CG11327|FBan0011327|CT31615|FBan0011327 99% Plus / Plus *F bs23b07.y1 AI946292 Contig097 CG5261|FBan0005261|CT42460|FBan0005261 99% Plus / Plus *F bs23b08.y1 AI946293 Contig093 LP11475.5prime 99% Plus / Plus CG15109|FBan0015109|CT34984|FBan0015109 99% Plus / Plus *F bs23b09.y1 AI946294 singleton LP09513.5prime 99% Plus / Plus CG1242|FBan0001242|CT2354|FBan0001242 100% Plus / Plus *F bs23b10.y1 AI946295 Contig467 GH04277.5prime 99% Plus / Plus CG6541|FBan0006541|CT20379|FBan0006541 99% Plus / Plus *F bs23b11.y1 AI946296 singleton CG4654|FBan0004654|CT15039|FBan0004654 99% Plus / Plus *F bs23b12.y1 AI946297 Contig321 GH19858.5prime 100% Plus / Plus CG10664|FBan0010664|CT29876|FBan0010664 100% Plus / Plus *F bs23c02.y1 AI946298 singleton CG5479|FBan0005479|CT17374|FBan0005479 99% Plus / Plus *F bs23c03.y1 AI946299 Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs23c04.y1 AI946300 singleton GH24548.5prime 99% Plus / Plus CG1958|FBan0001958|CT6090|FBan0001958 99% Plus / Plus *F bs23c05.y1 pending Contig400 LD29228.5prime 95% Plus / Minus CG12880|FBan0012880|CT32024|FBan0012880 95% Plus / Minus *F bs23c06.y1 AI946301 Contig519 GH24914.5prime 100% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs23c07.y1 AI946302 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs23c08.y1 AI946303 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 98% Plus / Plus *F bs23c09.y1 AI946304 singleton CG9748|FBan0009748|CT27543|FBan0009748 100% Plus / Plus *F bs23c10.y1 AI946305 Contig500 LD35407.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs23c11.y1 pending singleton LP02636.5prime 98% Plus / Plus CG1172|FBan0001172|CT2061|FBan0001172 98% Plus / Plus *F bs23c12.y1 AI946306 singleton CG12689|FBan0012689|CT35559|FBan0012689 99% Plus / Plus *F bs23d02.y1 AI946307 singleton CG4248|FBan0004248|CT10294|FBan0004248 99% Plus / Plus *F bs23d03.y1 AI946308 singleton GH27531.5prime 100% Plus / Plus CG6756|FBan0006756|CT20965|FBan0006756 100% Plus / Plus *F bs23d04.y1 pending Contig514 GH24664.5prime 98% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 98% Plus / Plus *F bs23d06.y1 AI946309 Contig538 GH26445.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs23d07.y1 AI946310 Contig408 GH17939.5prime 99% Plus / Plus CG4983|FBan0004983|CT15981|FBan0004983 99% Plus / Plus *F bs23d08.y1 AI946311 Contig088 CG7309|FBan0007309|CT22551|FBan0007309 100% Plus / Plus *F bs23d09.y1 AI946312 Contig495 GH18154.3prime 100% Plus / Minus CG9133|FBan0009133|CT10073|FBan0009133 100% Plus / Plus *F bs23d10.y1 AI946313 Contig506 GH20038.5prime 100% Plus / Plus CG18396|FBan0018396|CT41800|FBan0018396 100% Plus / Plus *F bs23d12.y1 pending singleton CG4707|FBan0004707|CT15167|FBan0004707 97% Plus / Plus *F bs23e01.y1 AI946314 Contig417 LP10240.5prime 99% Plus / Plus CG2955|FBan0002955|CT10021|FBan0002955 99% Plus / Plus *F bs23e02.y1 pending singleton CG11025|FBan0011025|CT30857|FBan0011025 97% Plus / Plus *F bs23e03.y1 AI946315 Contig099 CG5906|FBan0005906|CT18549|FBan0005906 99% Plus / Plus *F bs23e04.y1 AI946316 singleton CG11008|FBan0011008|CT30829|FBan0011008 100% Plus / Plus *F bs23e05.y1 AI946317 Contig497 GH14434.5prime 98% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 98% Plus / Plus *F bs23e06.y1 pending Contig492 GH15883.5prime 99% Plus / Plus CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs23e07.y1 AI946318 Contig469 CG8732|FBan0008732|CT25221|FBan0008732 100% Plus / Minus *F bs23e08.y1 AI946319 Contig324 CG8476|FBan0008476|CT24775|FBan0008476 99% Plus / Plus *F bs23e09.y1 AI946320 Contig536 GH02210.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs23e10.y1 pending singleton CG12263|FBan0012263|CT16577|FBan0012263 97% Plus / Minus *F bs23e11.y1 AI946321 singleton CG6657|FBan0006657|CT20658|FBan0006657 98% Plus / Plus *F bs23e12.y1 pending Contig537 GH10230.5prime 96% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 96% Plus / Plus *F bs23f01.y1 AI946322 singleton GH25406.5prime 100% Plus / Plus CG3161|FBan0003161|CT10607|FBan0003161 100% Plus / Plus *F bs23f02.y1 AI946323 Contig133 GH26702.5prime 99% Plus / Plus CG4669|FBan0004669|CT15079|FBan0004669 100% Plus / Plus *F bs23f03.y1 AI946324 Contig130 GH05253.5prime 99% Plus / Plus none none *F bs23f04.y1 AI946325 singleton none none *F bs23f05.y1 AI946326 Contig371 CG14305|FBan0014305|CT33935|FBan0014305 99% Plus / Plus *F bs23f06.y1 AI946327 Contig513 LP02115.5prime 100% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs23f07.y1 pending Contig536 GH02210.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs23f08.y1 AI946328 Contig487 CG12470|FBan0012470|CT32706|FBan0012470 100% Plus / Plus *F bs23f09.y1 AI946329 Contig540 none none *F bs23f11.y1 AI946330 Contig455 GH06904.5prime 100% Plus / Plus CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs23f12.y1 AI946331 singleton GH20963.5prime 99% Plus / Plus CG6661|FBan0006661|CT20682|FBan0006661 99% Plus / Plus *F bs23g01.y1 AI946332 singleton LD12948.5prime 100% Plus / Plus CG10855|FBan0010855|CT30391|FBan0010855 100% Plus / Plus *F bs23g02.y1 AI946333 singleton CK00152.5prime 98% Plus / Plus CG1768|FBan0001768|CT4728|FBan0001768 99% Plus / Plus *F bs23g03.y1 AI946334 Contig500 LD35407.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs23g04.y1 AI946335 Contig091 LP05168.3prime 100% Plus / Minus none none *F bs23g05.y1 AI946336 Contig521 GH07596.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs23g06.y1 AI946337 Contig471 none none *F bs23g07.y1 AI946338 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs23g08.y1 AI946339 singleton HL03165.5prime 99% Plus / Plus CG9949|FBan0009949|CT28005|FBan0009949 99% Plus / Plus *F bs23g09.y1 pending singleton GH15751.5prime 95% Plus / Plus CG1210|FBan0001210|CT42509|FBan0001210 95% Plus / Plus *F bs23g10.y1 AI946340 singleton none none *F bs23g11.y1 pending Contig177 GH19753.5prime 99% Plus / Plus CG3967|FBan0003967|CT39420|FBan0003967 99% Plus / Plus *F bs23g12.y1 AI946341 Contig542 GH21803.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs23h01.y1 AI946342 Contig427 CG18131|FBan0018131|CT40834|FBan0018131 99% Plus / Plus *F bs23h02.y1 pending Contig525 GH02147.5prime 98% Plus / Plus CG10219|FBan0010219|CT28735|FBan0010219 98% Plus / Plus *F bs23h03.y1 AI946343 Contig439 LD25555.5prime 99% Plus / Plus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs23h04.y1 pending singleton none none *F bs23h05.y1 pending Contig520 none none *F bs23h06.y1 pending Contig541 GH20441.5prime 97% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 95% Plus / Plus *F bs23h08.y1 AI946344 Contig247 GH06489.5prime 98% Plus / Plus CG17956|FBan0017956|CT40004|FBan0017956 98% Plus / Plus *F bs23h09.y1 AI946345 singleton CG17380|FBan0017380|CT38388|FBan0017380 99% Plus / Plus *F bs23h10.y1 AI946346 singleton LD25085.5prime 99% Plus / Plus CG8819|FBan0008819|CT25384|FBan0008819 99% Plus / Plus *F bs23h11.y1 AI946347 Contig528 GH06435.5prime 99% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs23h12.y1 AI946348 Contig104 GH14476.5prime 99% Plus / Plus CG3565|FBan0003565|CT11950|FBan0003565 99% Plus / Plus *F bs24a01.y1 AI946349 Contig504 CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs24a03.y1 pending Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs24a05.y1 AI946350 Contig507 GM13768.5prime 97% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs24a06.y1 pending Contig543 GH09553.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs24a09.y1 pending Contig523 LD23336.3prime 98% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs24a11.y1 AI946351 Contig329 CG10749|FBan0010749|CT30132|FBan0010749 97% Plus / Plus *F bs24a12.y1 AI946352 Contig107 GH25462.5prime 95% Plus / Plus none none *F bs24b01.y1 AI946353 singleton GH13107.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 100% Plus / Plus *F bs24b03.y1 AI946354 Contig195 LD11277.5prime 99% Plus / Plus CG6998|FBan0006998|CT21660|FBan0006998 99% Plus / Plus *F bs24b05.y1 AI946355 Contig455 CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs24b07.y1 pending singleton LD15549.5prime 97% Plus / Plus CG9135|FBan0009135|CT26178|FBan0009135 99% Plus / Plus *F bs24b09.y1 AI946356 singleton CG5693|FBan0005693|CT17948|FBan0005693 99% Plus / Plus *F bs24b10.y1 pending Contig458 GH26164.5prime 98% Plus / Plus CG16760|FBan0016760|CT8050|FBan0016760 98% Plus / Plus *F bs24b12.y1 pending singleton SD03066.5prime 98% Plus / Plus CG13868|FBan0013868|CT33390|FBan0013868 94% Plus / Plus *F bs24c02.y1 AI946357 Contig439 GH23957.5prime 100% Plus / Plus CG8994|FBan0008994|CT25812|FBan0008994 100% Plus / Plus *F bs24c03.y1 AI946358 Contig488 LP11873.5prime 99% Plus / Plus none none *F bs24c04.y1 AI946359 Contig209 GH28557.5prime 100% Plus / Plus CG7770|FBan0007770|CT23626|FBan0007770 100% Plus / Plus *F bs24c05.y1 AI946360 Contig131 GH18529.5prime 99% Plus / Plus CG8493|FBan0008493|CT24841|FBan0008493 100% Plus / Plus *F bs24c06.y1 pending Contig543 GH18035.5prime 98% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 100% Plus / Plus *F bs24c07.y1 AI946361 Contig526 GH11587.5prime 99% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs24c08.y1 pending Contig146 CG11861|FBan0011861|CT40006|FBan0011861 100% Plus / Plus *F bs24c09.y1 pending singleton CG5086|FBan0005086|CT16303|FBan0005086 90% Plus / Plus *F bs24c10.y1 pending Contig443 CG10734|FBan0010734|CT30089|FBan0010734 98% Plus / Plus *F bs24c11.y1 AI946362 Contig538 GH15626.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs24c12.y1 AI946363 Contig544 none none *F bs24d01.y1 AI946364 Contig112 CG2164|FBan0002164|CT7072|FBan0002164 98% Plus / Plus *F bs24d02.y1 AI946365 Contig542 SD06940.5prime 96% Plus / Minus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs24d03.y1 AI946366 singleton CG3532|FBan0003532|CT11878|FBan0003532 98% Plus / Plus *F bs24d04.y1 AI946367 Contig459 LP08448.5prime 100% Plus / Plus CG4323|FBan0004323|CT14141|FBan0004323 100% Plus / Plus *F bs24d05.y1 AI946368 singleton GH20229.3prime 96% Plus / Plus CG4286|FBan0004286|CT14051|FBan0004286 98% Plus / Plus *F bs24d06.y1 pending singleton GH10129.5prime 99% Plus / Plus CG9762|FBan0009762|CT27589|FBan0009762 99% Plus / Plus *F bs24d07.y1 pending Contig116 CG17564|FBan0017564|CT38791|FBan0017564 99% Plus / Plus *F bs24d09.y1 AI946369 Contig383 LD28345.5prime 99% Plus / Plus CG2512|FBan0002512|CT8333|FBan0002512 99% Plus / Plus *F bs24d10.y1 pending Contig434 CG7164|FBan0007164|CT22119|FBan0007164 100% Plus / Plus *F bs24d11.y1 AI946370 singleton GH12925.3prime 97% Plus / Minus CG2291|FBan0002291|CT7606|FBan0002291 95% Plus / Plus *F bs24d12.y1 pending Contig474 GH09553.5prime 97% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 97% Plus / Plus *F bs24e01.y1 AI946371 Contig118 GH28094.5prime 99% Plus / Plus CG17645|FBan0017645|CT38959|FBan0017645 99% Plus / Plus *F bs24e02.y1 AI946372 singleton LD07241.5prime 100% Plus / Plus none none *F bs24e03.y1 AI946373 Contig105 LD19790.5prime 99% Plus / Plus CG12051|FBan0012051|CT3675|FBan0012051 100% Plus / Plus *F bs24e04.y1 pending singleton GH15838.5prime 99% Plus / Minus CG6195|FBan0006195|CT19422|FBan0006195 99% Plus / Minus *F bs24e05.y1 AI946374 Contig102 CG17030|FBan0017030|CT33160|FBan0017030 99% Plus / Plus *F bs24e06.y1 AI946375 singleton CG11201|FBan0011201|CT31280|FBan0011201 100% Plus / Plus *F bs24e08.y1 pending Contig439 LD25555.5prime 99% Plus / Plus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs24e09.y1 AI946376 singleton none none *F bs24e10.y1 AI946377 singleton LP04474.5prime 100% Plus / Plus CG8257|FBan0008257|CT24479|FBan0008257 100% Plus / Plus *F bs24e11.y1 AI946378 Contig512 LP07747.5prime 98% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 98% Plus / Plus *F bs24f01.y1 pending Contig544 none none *F bs24f02.y1 pending Contig485 none none *F bs24f03.y1 pending Contig084 LP04278.5prime 98% Plus / Plus CG1837|FBan0001837|CT5608|FBan0001837 98% Plus / Plus *F bs24f04.y1 pending Contig537 GH07855.5prime 97% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 97% Plus / Plus *F bs24f05.y1 AI946379 Contig502 GH26978.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs24f06.y1 pending Contig488 CG13353|FBan0013353|CT32674|FBan0013353 94% Plus / Plus *F bs24f07.y1 AI946380 singleton LD06356.5prime 96% Plus / Plus CG10660|FBan0010660|CT29858|FBan0010660 97% Plus / Plus *F bs24f08.y1 AI946381 singleton none none *F bs24f09.y1 pending Contig346 LD16755.5prime 98% Plus / Plus CG8448|FBan0008448|CT37123|FBan0008448 99% Plus / Plus *F bs24f10.y1 pending singleton GH11476.5prime 99% Plus / Plus CG1979|FBan0001979|CT6207|FBan0001979 99% Plus / Plus *F bs24f11.y1 pending singleton CG9803|FBan0009803|CT27690|FBan0009803 100% Plus / Plus *F bs24f12.y1 AI946382 singleton CG12147|FBan0012147|CT8263|FBan0012147 100% Plus / Plus *F bs24g01.y1 AI946383 Contig117 GM02731.5prime 99% Plus / Minus CG4144|FBan0004144|CT13722|FBan0004144 100% Plus / Plus *F bs24g02.y1 AI946384 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 100% Plus / Plus *F bs24g04.y1 AI946385 singleton GH12755.5prime 100% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 100% Plus / Plus *F bs24g05.y1 pending Contig419 GH19789.5prime 99% Plus / Plus CG14740|FBan0014740|CT34534|FBan0014740 99% Plus / Plus *F bs24g06.y1 pending Contig322 CG6306|FBan0006306|CT19682|FBan0006306 94% Plus / Plus *F bs24g07.y1 pending Contig275 GH06247.3prime 99% Plus / Minus CG5684|FBan0005684|CT17938|FBan0005684 99% Plus / Plus *F bs24g08.y1 AI946386 singleton LP08011.5prime 98% Plus / Plus CG17725|FBan0017725|CT30577|FBan0017725 98% Plus / Plus *F bs24g09.y1 pending Contig132 CG6701|FBan0006701|CT20818|FBan0006701 99% Plus / Plus *F bs24g10.y1 pending singleton LP10419.5prime 99% Plus / Plus CG3140|FBan0003140|CT10496|FBan0003140 99% Plus / Plus *F bs24g11.y1 AI946387 Contig544 GH22513.5prime 97% Plus / Plus none none *F bs24h01.y1 pending singleton LD22825.3prime 100% Plus / Minus CG4248|FBan0004248|CT10294|FBan0004248 99% Plus / Plus *F bs24h03.y1 AI946388 Contig505 GH02250.5prime 100% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 100% Plus / Plus *F bs24h04.y1 AI946389 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs24h05.y1 AI946390 Contig520 none none *F bs24h06.y1 AI946391 Contig512 LP07747.5prime 97% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 97% Plus / Plus *F bs24h07.y1 pending Contig544 GH26094.5prime 99% Plus / Plus CG17470|FBan0017470|CT29160|FBan0017470 99% Plus / Plus *F bs24h08.y1 AI946392 Contig510 GH21762.5prime 99% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 99% Plus / Plus *F bs24h09.y1 pending Contig500 LD35818.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs24h10.y1 pending Contig444 LD14338.5prime 100% Plus / Plus CG2922|FBan0002922|CT7240|FBan0002922 100% Plus / Plus *F bs24h11.y1 AI946393 Contig320 GH12875.5prime 100% Plus / Plus CG7634|FBan0007634|CT23283|FBan0007634 100% Plus / Plus *F bs24h12.y1 pending singleton CG6790|FBan0006790|CT21071|FBan0006790 100% Plus / Plus *F bs25a01.y1 pending Contig425 CG17344|FBan0017344|CT35082|FBan0017344 96% Plus / Plus *F bs25a03.y1 AI946394 Contig463 CG10690|FBan0010690|CT29964|FBan0010690 100% Plus / Minus *F bs25a04.y1 pending Contig397 CG11861|FBan0011861|CT40006|FBan0011861 99% Plus / Plus *F bs25a05.y1 AI946395 Contig515 LD41653.5prime 100% Plus / Plus none none *F bs25a06.y1 AI946396 Contig457 GH18334.5prime 99% Plus / Plus CG6255|FBan0006255|CT19574|FBan0006255 100% Plus / Plus *F bs25a07.y1 pending singleton CG10345|FBan0010345|CT29054|FBan0010345 94% Plus / Plus *F bs25a10.y1 AI946397 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 98% Plus / Plus *F bs25a11.y1 pending Contig321 GH12425.5prime 100% Plus / Plus CG10664|FBan0010664|CT29876|FBan0010664 100% Plus / Plus *F bs25a12.y1 pending singleton GH25081.3prime 99% Plus / Minus CG11773|FBan0011773|CT36855|FBan0011773 99% Plus / Plus *F bs25b01.y1 pending Contig100 SD10949.3prime 99% Plus / Minus CG10219|FBan0010219|CT28735|FBan0010219 99% Plus / Plus *F bs25b02.y1 AI946398 Contig543 GH18035.5prime 100% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 100% Plus / Plus *F bs25b03.y1 pending singleton CG8575|FBan0008575|CT8689|FBan0008575 99% Plus / Plus *F bs25b05.y1 AI946399 Contig482 CG18449|FBan0018449|CT42026|FBan0018449 99% Plus / Plus *F bs25b06.y1 pending singleton CG11043|FBan0011043|CT30188|FBan0011043 99% Plus / Plus *F bs25b07.y1 pending singleton none none *F bs25b08.y1 AI946400 Contig186 GH22005.3prime 96% Plus / Minus CG10120|FBan0010120|CT38328|FBan0010120 96% Plus / Plus *F bs25b09.y1 pending singleton GH24521.5prime 100% Plus / Plus CG14041|FBan0014041|CT33600|FBan0014041 100% Plus / Plus *F bs25b10.y1 AI946401 Contig390 LP11692.5prime 100% Plus / Plus CG9975|FBan0009975|CT28093|FBan0009975 100% Plus / Plus *F bs25b11.y1 AI946402 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs25b12.y1 AI946403 Contig497 GH14434.5prime 98% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 99% Plus / Plus *F bs25c01.y1 AI946404 Contig514 GH24336.5prime 99% Plus / Plus CG13612|FBan0013612|CT32997|FBan0013612 99% Plus / Plus *F bs25c03.y1 AI946405 singleton LD28310.5prime 100% Plus / Plus CG4443|FBan0004443|CT14438|FBan0004443 100% Plus / Plus *F bs25c06.y1 pending singleton LP01788.5prime 100% Plus / Plus CG7107|FBan0007107|CT21965|FBan0007107 99% Plus / Plus *F bs25c07.y1 AI946406 Contig142 none none *F bs25c08.y1 AI946407 singleton SD07141.5prime 99% Plus / Plus CG10954|FBan0010954|CT30673|FBan0010954 99% Plus / Plus *F bs25c09.y1 AI946408 Contig536 GH02210.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 97% Plus / Plus *F bs25c10.y1 AI946409 Contig392 GH10365.5prime 100% Plus / Plus CG17238|FBan0017238|CT21085|FBan0017238 100% Plus / Plus *F bs25c11.y1 AI946410 singleton GH24521.5prime 99% Plus / Plus CG14041|FBan0014041|CT33600|FBan0014041 99% Plus / Plus *F bs25c12.y1 AI946411 singleton none none *F bs25d01.y1 pending Contig460 GH10940.5prime 95% Plus / Plus CG18662|FBan0018662|CT42585|FBan0018662 95% Plus / Minus *F bs25d03.y1 AI946412 Contig485 none none *F bs25d05.y1 AI946413 singleton GM07780.5prime 100% Plus / Plus none none *F bs25d06.y1 AI946414 singleton CG11052|FBan0011052|CT30919|FBan0011052 100% Plus / Plus *F bs25d08.y1 AI946415 Contig544 GH10815.5prime 99% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs25d10.y1 AI946416 Contig141 none none *F bs25d11.y1 AI946417 singleton CG7024|FBan0007024|CT21720|FBan0007024 100% Plus / Plus *F bs25d12.y1 AI946418 Contig322 GH12946.3prime 97% Plus / Minus CG6306|FBan0006306|CT19682|FBan0006306 98% Plus / Plus *F bs25e02.y1 pending Contig493 none none *F bs25e05.y1 AI946419 singleton GM05005.5prime 95% Plus / Plus CG10756|FBan0010756|CT30146|FBan0010756 97% Plus / Plus *F bs25e07.y1 AI946420 Contig502 GH01042.5prime 97% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 97% Plus / Plus *F bs25e08.y1 pending singleton LD33067.3prime 92% Plus / Minus none none *F bs25e09.y1 AI946421 Contig537 GH10230.5prime 100% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 100% Plus / Plus *F bs25e10.y1 AI946422 singleton none none *F bs25e11.y1 AI946423 singleton CG2093|FBan0002093|CT6820|FBan0002093 100% Plus / Minus *F bs25e12.y1 AI946424 singleton CK01411.5prime 98% Plus / Plus CG12736|FBan0012736|CT4271|FBan0012736 100% Plus / Plus *F bs25f01.y1 pending Contig319 GH18530.5prime 100% Plus / Plus CG10143|FBan0010143|CT28535|FBan0010143 100% Plus / Plus *F bs25f02.y1 AI946425 Contig318 GH14384.5prime 99% Plus / Plus CG9131|FBan0009131|CT26166|FBan0009131 99% Plus / Plus *F bs25f04.y1 AI946426 singleton GH22960.5prime 99% Plus / Plus CG1475|FBan0001475|CT2508|FBan0001475 99% Plus / Plus *F bs25f06.y1 pending Contig330 LP07429.5prime 100% Plus / Plus CG3222|FBan0003222|CT10697|FBan0003222 100% Plus / Plus *F bs25f07.y1 AI946427 Contig380 CG13778|FBan0013778|CT33266|FBan0013778 98% Plus / Minus *F bs25f08.y1 pending singleton GM05842.5prime 99% Plus / Plus CG6174|FBan0006174|CT19354|FBan0006174 99% Plus / Plus *F bs25f09.y1 AI946428 Contig233 CG3354|FBan0003354|CT11217|FBan0003354 92% Plus / Plus *F bs25f10.y1 AI946429 Contig503 GH11521.5prime 96% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 96% Plus / Plus *F bs25f11.y1 AI946430 Contig137 CG7337|FBan0007337|CT10216|FBan0007337 100% Plus / Plus *F bs25g05.y1 AI946431 Contig263 GM13138.5prime 99% Plus / Plus CG12051|FBan0012051|CT3675|FBan0012051 99% Plus / Plus *F bs25g06.y1 AI946432 Contig542 GH22924.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs25g07.y1 AI946433 singleton GM08807.5prime 97% Plus / Plus CG8525|FBan0008525|CT24905|FBan0008525 98% Plus / Plus *F bs25g08.y1 AI946434 Contig518 GH22658.5prime 97% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs25g09.y1 AI946435 Contig459 LP06931.5prime 98% Plus / Plus CG4323|FBan0004323|CT14141|FBan0004323 99% Plus / Plus *F bs25g10.y1 AI946436 Contig387 GH13802.5prime 98% Plus / Plus CG1314|FBan0001314|CT2886|FBan0001314 99% Plus / Plus *F bs25g11.y1 AI946437 singleton LP05407.5prime 100% Plus / Plus CG5442|FBan0005442|CT37788|FBan0005442 100% Plus / Plus *F bs25g12.y1 AI946438 singleton none none *F bs25h02.y1 pending Contig412 GH14048.5prime 100% Plus / Plus CG7131|FBan0007131|CT22049|FBan0007131 100% Plus / Plus *F bs25h03.y1 pending Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs25h04.y1 AI946439 singleton GH06679.5prime 96% Plus / Plus CG1662|FBan0001662|CT4618|FBan0001662 96% Plus / Plus *F bs25h05.y1 AI946440 singleton SD10324.5prime 100% Plus / Plus CG6647|FBan0006647|CT20634|FBan0006647 100% Plus / Plus *F bs25h06.y1 AI946441 Contig531 GH04958.5prime 99% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs25h08.y1 AI946442 singleton CG8608|FBan0008608|CT14550|FBan0008608 99% Plus / Plus *F bs25h09.y1 pending Contig519 GH07296.5prime 100% Plus / Plus CG5762|FBan0005762|CT18086|FBan0005762 100% Plus / Plus *F bs25h11.y1 AI946443 singleton none none *F bs25h12.y1 AI946444 singleton LP04257.3prime 99% Plus / Minus CG4911|FBan0004911|CT15774|FBan0004911 99% Plus / Plus *F bs26a07.y1 AI946445 singleton GH27389.5prime 100% Plus / Plus CG6565|FBan0006565|CT20432|FBan0006565 100% Plus / Plus *F bs26a08.y1 AI946446 Contig337 GH07823.3prime 98% Plus / Minus CG8430|FBan0008430|CT18945|FBan0008430 98% Plus / Plus *F bs26a09.y1 AI946447 Contig171 CG12983|FBan0012983|CT32175|FBan0012983 100% Plus / Plus *F bs26a11.y1 AI946448 Contig317 CG9570|FBan0009570|CT17340|FBan0009570 100% Plus / Plus *F bs26b07.y1 AI946449 Contig535 GH03745.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs26b08.y1 AI946450 Contig540 none none *F bs26b09.y1 AI946451 singleton CG9495|FBan0009495|CT26890|FBan0009495 99% Plus / Minus *F bs26b10.y1 AI946452 singleton CG5461|FBan0005461|CT17310|FBan0005461 100% Plus / Plus *F bs26b12.y1 AI946453 Contig516 GH12755.5prime 100% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 100% Plus / Plus *F bs26c07.y1 AI946454 Contig172 CG5987|FBan0005987|CT18801|FBan0005987 99% Plus / Plus *F bs26c08.y1 AI946455 singleton CG2164|FBan0002164|CT7072|FBan0002164 99% Plus / Plus *F bs26c09.y1 AI946456 Contig176 HL01628.5prime 100% Plus / Plus CG6084|FBan0006084|CT19142|FBan0006084 100% Plus / Plus *F bs26c10.y1 AI946457 singleton LD21829.5prime 98% Plus / Plus CG14647|FBan0014647|CT34419|FBan0014647 100% Plus / Minus *F bs26c11.y1 AI946458 Contig492 GH10725.5prime 99% Plus / Plus CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs26c12.y1 AI946459 Contig153 CG8277|FBan0008277|CT24507|FBan0008277 98% Plus / Plus *F bs26d07.y1 AI946460 Contig260 GH04483.5prime 100% Plus / Plus CG6815|FBan0006815|CT19832|FBan0006815 100% Plus / Plus *F bs26d08.y1 AI946461 singleton CG10734|FBan0010734|CT30089|FBan0010734 98% Plus / Plus *F bs26d09.y1 AI946462 Contig499 GH19122.3prime 100% Plus / Minus CG18184|FBan0018184|CT41084|FBan0018184 100% Plus / Plus *F bs26d11.y1 AI946463 Contig279 GH22225.3prime 99% Plus / Minus CG3581|FBan0003581|CT12033|FBan0003581 99% Plus / Plus *F bs26d12.y1 AI946464 Contig414 none none *F bs26e07.y1 pending singleton none none *F bs26e08.y1 AI946465 singleton CG16739|FBan0016739|CT33395|FBan0016739 99% Plus / Plus *F bs26e09.y1 AI946466 Contig181 CG8220|FBan0008220|CT23699|FBan0008220 100% Plus / Plus *F bs26e10.y1 AI946467 Contig536 GH02210.5prime 97% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs26e11.y1 AI946468 Contig419 GH19789.5prime 97% Plus / Plus CG14740|FBan0014740|CT34534|FBan0014740 97% Plus / Plus *F bs26e12.y1 AI946469 Contig490 LP03962.5prime 100% Plus / Plus CG13263|FBan0013263|CT32547|FBan0013263 100% Plus / Plus *F bs26f07.y1 AI946470 Contig331 CG17717|FBan0017717|CT39281|FBan0017717 99% Plus / Plus *F bs26f08.y1 AI946471 Contig205 CG5043|FBan0005043|CT16181|FBan0005043 100% Plus / Plus *F bs26f10.y1 AI946472 singleton LP04853.5prime 99% Plus / Plus CG3000|FBan0003000|CT10109|FBan0003000 99% Plus / Plus *F bs26f11.y1 AI946473 singleton GH09018.5prime 99% Plus / Plus CG1782|FBan0001782|CT5340|FBan0001782 100% Plus / Plus *F bs26f12.y1 AI946474 singleton LD15245.5prime 99% Plus / Plus CG6866|FBan0006866|CT37411|FBan0006866 100% Plus / Plus *F bs26g07.y1 AI946475 Contig513 LP02115.5prime 100% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs26g08.y1 AI946476 singleton LD36024.5prime 96% Plus / Plus CG6398|FBan0006398|CT19938|FBan0006398 100% Plus / Plus *F bs26g09.y1 AI946477 singleton none none *F bs26g10.y1 AI946478 singleton CG14839|FBan0014839|CT34655|FBan0014839 99% Plus / Plus *F bs26g11.y1 AI946479 Contig409 none none *F bs26g12.y1 AI946480 singleton CG6301|FBan0006301|CT19708|FBan0006301 100% Plus / Plus *F bs26h07.y1 AI946481 singleton CG11710|FBan0011710|CT35524|FBan0011710 99% Plus / Plus *F bs26h08.y1 AI946482 Contig497 GH14434.5prime 98% Plus / Plus CG11624|FBan0011624|CT36603|FBan0011624 98% Plus / Plus *F bs26h09.y1 AI946483 Contig196 GH07208.5prime 100% Plus / Plus CG3127|FBan0003127|CT10484|FBan0003127 100% Plus / Plus *F bs26h10.y1 AI946484 Contig285 CG7701|FBan0007701|CT23463|FBan0007701 98% Plus / Plus *F bs26h11.y1 AI946485 singleton CG17216|FBan0017216|CT38153|FBan0017216 100% Plus / Plus *F bs26h12.y1 AI946486 Contig475 LP02384.5prime 100% Plus / Plus CG7931|FBan0007931|CT6483|FBan0007931 97% Plus / Plus *F bs27a01.y1 AI946487 Contig261 CK02305.5prime 100% Plus / Plus CG17949|FBan0017949|CT39990|FBan0017949 97% Plus / Plus *F bs27a02.y1 AI946488 singleton none none *F bs27a03.y1 AI946489 singleton GH05075.3prime 99% Plus / Plus CG17870|FBan0017870|CT39748|FBan0017870 93% Plus / Minus *F bs27a04.y1 AI946490 singleton LD15117.5prime 99% Plus / Plus CG3825|FBan0003825|CT12745|FBan0003825 99% Plus / Plus *F bs27a05.y1 AI946491 singleton GH12681.5prime 98% Plus / Plus CG12131|FBan0012131|CT7734|FBan0012131 100% Plus / Plus *F bs27a06.y1 AI946492 singleton CG11247|FBan0011247|CT31391|FBan0011247 100% Plus / Plus *F bs27a07.y1 AI946493 Contig466 GH17555.5prime 99% Plus / Plus CG2267|FBan0002267|CT7048|FBan0002267 99% Plus / Plus *F bs27a08.y1 AI946494 Contig288 SD02150.5prime 100% Plus / Plus CG1655|FBan0001655|CT4502|FBan0001655 98% Plus / Plus *F bs27a09.y1 AI946495 singleton LD06308.5prime 100% Plus / Plus CG2201|FBan0002201|CT7236|FBan0002201 100% Plus / Plus *F bs27a10.y1 AI946496 Contig457 GH18334.5prime 99% Plus / Plus CG6255|FBan0006255|CT19574|FBan0006255 99% Plus / Plus *F bs27a11.y1 pending Contig198 CG1902|FBan0001902|CT5564|FBan0001902 99% Plus / Plus *F bs27a12.y1 AI946497 Contig498 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT38382|FBan0018461 97% Plus / Plus *F bs27b02.y1 AI946498 singleton CG5421|FBan0005421|CT17198|FBan0005421 98% Plus / Plus *F bs27b03.y1 AI946499 singleton GH23536.5prime 99% Plus / Plus CG11779|FBan0011779|CT17728|FBan0011779 99% Plus / Plus *F bs27b04.y1 AI946500 Contig543 GH04837.5prime 97% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 97% Plus / Plus *F bs27b05.y1 AI946501 Contig318 GH14384.5prime 99% Plus / Plus CG9131|FBan0009131|CT26166|FBan0009131 99% Plus / Plus *F bs27b06.y1 AI946502 Contig534 none none *F bs27b07.y1 AI946503 Contig542 GH21803.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs27b08.y1 AI946504 Contig543 CG17934|FBan0017934|CT39960|FBan0017934 100% Plus / Plus *F bs27b09.y1 AI946505 Contig425 CG17344|FBan0017344|CT35082|FBan0017344 98% Plus / Plus *F bs27b10.y1 AI946506 Contig233 CG3354|FBan0003354|CT11217|FBan0003354 91% Plus / Plus *F bs27b11.y1 AI946507 singleton GH02613.5prime 99% Plus / Plus CG15218|FBan0015218|CT35154|FBan0015218 99% Plus / Plus *F bs27c01.y1 AI946508 Contig533 GH09435.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs27c02.y1 AI946509 Contig479 GH28038.5prime 97% Plus / Plus CG5735|FBan0005735|CT18021|FBan0005735 95% Plus / Plus *F bs27c03.y1 AI946510 Contig358 GH25818.5prime 99% Plus / Plus CG7815|FBan0007815|CT23740|FBan0007815 99% Plus / Plus *F bs27c04.y1 AI946511 singleton GH21936.5prime 99% Plus / Plus CG1640|FBan0001640|CT4458|FBan0001640 99% Plus / Plus *F bs27c05.y1 AI946512 Contig360 GH23889.5prime 98% Plus / Plus CG5106|FBan0005106|CT15693|FBan0005106 99% Plus / Plus *F bs27c06.y1 AI946513 Contig524 GH15533.5prime 100% Plus / Plus CG9391|FBan0009391|CT26653|FBan0009391 100% Plus / Plus *F bs27c07.y1 AI946514 singleton none none *F bs27c08.y1 AI946515 singleton none none *F bs27c09.y1 AI946516 singleton LD05778.5prime 100% Plus / Plus CG10326|FBan0010326|CT28992|FBan0010326 100% Plus / Plus *F bs27c10.y1 pending Contig442 CG5024|FBan0005024|CT16147|FBan0005024 97% Plus / Plus *F bs27c11.y1 AI946517 singleton LD41983.5prime 100% Plus / Plus CG6258|FBan0006258|CT19604|FBan0006258 100% Plus / Plus *F bs27c12.y1 AI946518 Contig319 CG10143|FBan0010143|CT28535|FBan0010143 98% Plus / Plus *F bs27d01.y1 AI946519 Contig522 LP09246.5prime 99% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 100% Plus / Plus *F bs27d02.y1 pending singleton none none *F bs27d03.y1 AI946520 Contig316 GH25858.5prime 100% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 100% Plus / Plus *F bs27d04.y1 AI946521 Contig435 LP05191.5prime 100% Plus / Plus CG12562|FBan0012562|CT34305|FBan0012562 100% Plus / Plus *F bs27d05.y1 AI946522 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs27d06.y1 AI946523 singleton LD02189.5prime 99% Plus / Plus CG10272|FBan0010272|CT28863|FBan0010272 100% Plus / Plus *F bs27d07.y1 AI946524 singleton SD08388.5prime 98% Plus / Plus CG8776|FBan0008776|CT25308|FBan0008776 97% Plus / Plus *F bs27d08.y1 AI946525 singleton GH02051.5prime 96% Plus / Plus CG9359|FBan0009359|CT26587|FBan0009359 96% Plus / Plus *F bs27d09.y1 AI946526 Contig432 LP05778.5prime 100% Plus / Plus CG8938|FBan0008938|CT25660|FBan0008938 100% Plus / Plus *F bs27d10.y1 AI946527 Contig516 GH12755.5prime 100% Plus / Plus CG3982|FBan0003982|CT13237|FBan0003982 100% Plus / Plus *F bs27d11.y1 AI946528 Contig152 none none *F bs27d12.y1 AI946529 Contig481 CG5051|FBan0005051|CT16124|FBan0005051 100% Plus / Plus *F bs27e01.y1 AI946530 Contig542 GH21096.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs27e02.y1 AI946531 singleton none none *F bs27e03.y1 AI946532 Contig520 none none *F bs27e04.y1 pending singleton LD40023.3prime 99% Plus / Minus CG3941|FBan0003941|CT41931|FBan0003941 100% Plus / Plus *F bs27e05.y1 AI946533 Contig214 GH03629.5prime 100% Plus / Plus CG9803|FBan0009803|CT27690|FBan0009803 100% Plus / Plus *F bs27e06.y1 AI946534 Contig532 LP11026.5prime 100% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 100% Plus / Plus *F bs27e07.y1 AI946535 singleton GH26888.5prime 99% Plus / Plus CG17608|FBan0017608|CT32749|FBan0017608 100% Plus / Plus *F bs27e08.y1 AI946536 Contig216 CG2337|FBan0002337|CT7790|FBan0002337 98% Plus / Plus *F bs27e09.y1 AI946537 Contig408 GH17939.5prime 97% Plus / Plus CG4983|FBan0004983|CT15981|FBan0004983 97% Plus / Plus *F bs27e10.y1 AI946538 singleton CG15165|FBan0015165|CT35066|FBan0015165 100% Plus / Plus *F bs27e11.y1 AI946539 singleton none none *F bs27e12.y1 AI946540 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 98% Plus / Plus *F bs27f01.y1 AI946541 Contig450 GH23475.5prime 100% Plus / Plus none none *F bs27f02.y1 AI946542 singleton CG10682|FBan0010682|CT29912|FBan0010682 99% Plus / Plus *F bs27f03.y1 AI946543 singleton none none *F bs27f04.y1 AI946544 Contig320 GH12875.5prime 100% Plus / Plus CG7634|FBan0007634|CT23283|FBan0007634 100% Plus / Plus *F bs27f05.y1 AI946545 Contig531 GH04958.5prime 98% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs27f06.y1 AI946546 Contig395 LD07629.5prime 99% Plus / Plus CG8472|FBan0008472|CT24787|FBan0008472 99% Plus / Plus *F bs27f07.y1 AI946547 Contig328 GH19655.5prime 100% Plus / Plus CG15208|FBan0015208|CT35139|FBan0015208 100% Plus / Plus *F bs27f08.y1 AI946548 Contig423 GM02127.5prime 100% Plus / Plus CG1383|FBan0001383|CT3168|FBan0001383 100% Plus / Plus *F bs27f09.y1 pending singleton LD32004.3prime 99% Plus / Plus CG8409|FBan0008409|CT24689|FBan0008409 99% Plus / Plus *F bs27f10.y1 AI946549 singleton none none *F bs27f11.y1 AI946550 Contig543 GH09553.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs27f12.y1 AI946551 singleton LP10814.5prime 100% Plus / Plus none none *F bs27g01.y1 AI946552 Contig224 none none *F bs27g02.y1 AI946553 Contig520 none none *F bs27g03.y1 AI946554 Contig527 none none *F bs27g04.y1 AI946555 Contig543 GH11908.3prime 93% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 93% Plus / Minus *F bs27g05.y1 AI946556 Contig446 LP09632.5prime 100% Plus / Plus CG3691|FBan0003691|CT12381|FBan0003691 100% Plus / Plus *F bs27g06.y1 AI946557 Contig237 CG9389|FBan0009389|CT26645|FBan0009389 100% Plus / Minus *F bs27g07.y1 AI946558 singleton LD08328.5prime 98% Plus / Plus CG11986|FBan0011986|CT32134|FBan0011986 99% Plus / Plus *F bs27g08.y1 AI946559 Contig538 GH09790.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs27g09.y1 AI946560 singleton none none *F bs27g10.y1 AI946561 Contig167 GH25750.5prime 99% Plus / Plus CG14721|FBan0014721|CT34513|FBan0014721 99% Plus / Plus *F bs27g11.y1 AI946562 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 100% Plus / Plus *F bs27g12.y1 AI946563 singleton LD47767.5prime 99% Plus / Plus CG6598|FBan0006598|CT20508|FBan0006598 99% Plus / Plus *F bs27h01.y1 AI946564 Contig118 GH18848.3prime 81% Plus / Minus CG17645|FBan0017645|CT38959|FBan0017645 100% Plus / Plus *F bs27h02.y1 pending Contig227 GH10330.5prime 99% Plus / Plus CG3494|FBan0003494|CT11751|FBan0003494 99% Plus / Plus *F bs27h03.y1 AI946565 singleton CG8339|FBan0008339|CT42364|FBan0008339 99% Plus / Plus *F bs27h04.y1 AI946566 singleton CG18368|FBan0018368|CT41747|FBan0018368 98% Plus / Minus *F bs27h05.y1 AI946567 singleton none none *F bs27h06.y1 pending Contig408 GH17939.5prime 99% Plus / Plus CG4983|FBan0004983|CT15981|FBan0004983 98% Plus / Plus *F bs27h07.y1 AI946568 Contig532 GH28719.5prime 97% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs27h08.y1 AI946569 Contig414 CG12853|FBan0012853|CT31992|FBan0012853 100% Plus / Plus *F bs27h09.y1 AI946570 Contig503 GH11521.5prime 96% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 100% Plus / Plus *F bs27h10.y1 AI946571 Contig542 GH21803.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs27h11.y1 AI946572 Contig544 none none *F bs27h12.y1 AI946573 singleton GH07596.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs28a01.y1 AI946574 Contig178 CG7886|FBan0007886|CT23840|FBan0007886 100% Plus / Plus *F bs28a02.y1 AI946575 Contig085 none none *F bs28a03.y1 AI946576 singleton SD04586.5prime 99% Plus / Plus CG9531|FBan0009531|CT26970|FBan0009531 99% Plus / Plus *F bs28a04.y1 AI946577 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs28a05.y1 AI946578 singleton CG13337|FBan0013337|CT32657|FBan0013337 100% Plus / Plus *F bs28a06.y1 AI946579 Contig381 GH16751.5prime 98% Plus / Plus CG5735|FBan0005735|CT18021|FBan0005735 98% Plus / Plus *F bs28a07.y1 AI946580 Contig542 GH22924.5prime 97% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs28a08.y1 pending Contig388 LP10048.5prime 99% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs28a09.y1 AI946581 Contig181 GH19331.5prime 99% Plus / Plus CG8220|FBan0008220|CT23699|FBan0008220 100% Plus / Plus *F bs28a10.y1 AI946582 singleton CG13187|FBan0013187|CT32428|FBan0013187 100% Plus / Plus *F bs28a11.y1 AI946583 singleton CG8084|FBan0008084|CT4622|FBan0008084 99% Plus / Plus *F bs28b01.y1 AI946584 Contig236 LD36380.5prime 99% Plus / Plus CG9012|FBan0009012|CT25886|FBan0009012 99% Plus / Plus *F bs28b02.y1 pending singleton GH20954.5prime 99% Plus / Plus CG5565|FBan0005565|CT17332|FBan0005565 99% Plus / Plus *F bs28b03.y1 AI946585 Contig534 none none *F bs28b04.y1 AI946586 singleton LD21131.3prime 99% Plus / Minus CG8938|FBan0008938|CT25660|FBan0008938 99% Plus / Plus *F bs28b05.y1 pending Contig525 none none *F bs28b06.y1 AI946587 Contig487 CG12470|FBan0012470|CT32706|FBan0012470 100% Plus / Plus *F bs28b07.y1 pending Contig326 LP11903.5prime 96% Plus / Plus CG1534|FBan0001534|CT3947|FBan0001534 99% Plus / Plus *F bs28b08.y1 pending singleton CK01438.5prime.contig 97% Plus / Plus none none *F bs28b09.y1 AI946588 Contig335 GH15950.5prime 99% Plus / Plus CG4692|FBan0004692|CT15135|FBan0004692 99% Plus / Plus *F bs28b10.y1 AI946589 Contig540 GH25305.5prime 99% Plus / Plus CG9602|FBan0009602|CT27154|FBan0009602 99% Plus / Plus *F bs28b11.y1 pending Contig103 LP02209.3prime 98% Plus / Minus CG13993|FBan0013993|CT33549|FBan0013993 98% Plus / Plus *F bs28b12.y1 AI946590 Contig230 CG6209|FBan0006209|CT19464|FBan0006209 99% Plus / Plus *F bs28c01.y1 AI946591 Contig248 LD15110.5prime 89% Plus / Plus CG5265|FBan0005265|CT16817|FBan0005265 100% Plus / Plus *F bs28c02.y1 AI946592 Contig148 CG4631|FBan0004631|CT14966|FBan0004631 97% Plus / Plus *F bs28c03.y1 AI946593 singleton GH07389.5prime 99% Plus / Plus CG8084|FBan0008084|CT4622|FBan0008084 99% Plus / Plus *F bs28c04.y1 AI946594 singleton GH10480.5prime 99% Plus / Plus CG11963|FBan0011963|CT32142|FBan0011963 99% Plus / Plus *F bs28c05.y1 AI946595 Contig511 GH22851.5prime 98% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs28c06.y1 AI946596 Contig115 CG1338|FBan0001338|CT2966|FBan0001338 98% Plus / Plus *F bs28c07.y1 pending singleton LD33247.5prime 82% Plus / Plus CG4569|FBan0004569|CT14804|FBan0004569 99% Plus / Plus *F bs28c08.y1 AI946597 singleton LP06756.5prime 100% Plus / Plus none none *F bs28c09.y1 AI946598 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs28c10.y1 AI946599 Contig146 CG11861|FBan0011861|CT40006|FBan0011861 100% Plus / Plus *F bs28c11.y1 AI946600 singleton LD29996.5prime 98% Plus / Minus CG11999|FBan0011999|CT1038|FBan0011999 100% Plus / Minus *F bs28c12.y1 AI946601 Contig498 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT38382|FBan0018461 97% Plus / Plus *F bs28d02.y1 AI946602 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs28d03.y1 pending Contig504 CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs28d04.y1 pending singleton GH27033.5prime 100% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs28d05.y1 AI946603 Contig320 GH12875.5prime 99% Plus / Plus CG7634|FBan0007634|CT23283|FBan0007634 99% Plus / Plus *F bs28e01.y1 pending Contig471 none none *F bs28e02.y1 pending Contig161 LD40711.5prime 99% Plus / Plus CG11562|FBan0011562|CT33146|FBan0011562 99% Plus / Plus *F bs28e06.y1 pending singleton none none *F bs28e09.y1 pending Contig442 CG5024|FBan0005024|CT16147|FBan0005024 97% Plus / Plus *F bs28f01.y1 AI946604 Contig488 GH11373.5prime 100% Plus / Plus none none *F bs28f02.y1 pending singleton none none *F bs28f03.y1 AI946605 Contig474 GH09553.5prime 97% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 97% Plus / Plus *F bs28f04.y1 AI946606 Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs28f06.y1 pending singleton GM04812.5prime 99% Plus / Plus CG8297|FBan0008297|CT20148|FBan0008297 99% Plus / Plus *F bs28f07.y1 AI946607 singleton CG1979|FBan0001979|CT6207|FBan0001979 100% Plus / Plus *F bs28f08.y1 AI946608 singleton CG2996|FBan0002996|CT7882|FBan0002996 100% Plus / Plus *F bs28f09.y1 AI946609 singleton LD12308.5prime 97% Plus / Plus CG3171|FBan0003171|CT10621|FBan0003171 100% Plus / Plus *F bs28f11.y1 AI946610 singleton CG4712|FBan0004712|CT15189|FBan0004712 98% Plus / Plus *F bs28f12.y1 AI946611 singleton LD29159.5prime 99% Plus / Plus CG14613|FBan0014613|CT34370|FBan0014613 99% Plus / Plus *F bs28g01.y1 pending singleton CG14757|FBan0014757|CT34559|FBan0014757 99% Plus / Plus *F bs28g02.y1 pending Contig543 GH11908.5prime 99% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Plus *F bs28g03.y1 AI946612 Contig123 CG9455|FBan0009455|CT11129|FBan0009455 100% Plus / Plus *F bs28g04.y1 AI946613 Contig253 CG15878|FBan0015878|CT33288|FBan0015878 100% Plus / Plus *F bs28g05.y1 AI946614 Contig183 none none *F bs28g06.y1 AI946615 singleton LD13220.5prime 98% Plus / Plus CG11896|FBan0011896|CT37052|FBan0011896 99% Plus / Plus *F bs28g07.y1 pending singleton LP03480.5prime 99% Plus / Plus CG11840|FBan0011840|CT33129|FBan0011840 99% Plus / Plus *F bs28g08.y1 AI946616 Contig533 GH18778.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs28g09.y1 AI946617 Contig156 none none *F bs28g10.y1 AI946618 Contig344 CG7094|FBan0007094|CT21551|FBan0007094 100% Plus / Plus *F bs28g11.y1 AI946619 Contig329 CG10749|FBan0010749|CT30132|FBan0010749 97% Plus / Plus *F bs28g12.y1 AI946620 Contig249 CG5432|FBan0005432|CT17240|FBan0005432 96% Plus / Plus *F bs28h01.y1 AI946621 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs28h02.y1 AI946622 Contig452 GH15825.5prime 99% Plus / Plus CG3085|FBan0003085|CT10370|FBan0003085 99% Plus / Plus *F bs28h03.y1 AI946623 singleton LD34353.5prime 89% Plus / Plus CG9476|FBan0009476|CT26846|FBan0009476 100% Plus / Plus *F bs28h04.y1 AI946624 singleton CG15576|FBan0015576|CT35693|FBan0015576 100% Plus / Plus *F bs28h05.y1 AI946625 singleton CG8272|FBan0008272|CT8327|FBan0008272 100% Plus / Plus *F bs28h06.y1 AI946626 singleton GH28719.5prime 97% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs28h07.y1 AI946627 Contig315 GH19904.5prime 99% Plus / Plus CG7425|FBan0007425|CT22777|FBan0007425 100% Plus / Plus *F bs28h08.y1 pending Contig367 none none *F bs28h09.y1 AI946628 Contig534 CK00324.contig 97% Plus / Plus CG1827|FBan0001827|CT5558|FBan0001827 98% Plus / Plus *F bs28h10.y1 AI946629 Contig345 LP03157.5prime 99% Plus / Plus CG9412|FBan0009412|CT37417|FBan0009412 100% Plus / Plus *F bs28h12.y1 AI946630 Contig516 CG3982|FBan0003982|CT13237|FBan0003982 98% Plus / Plus *F bs29a02.y1 AI946631 singleton GH06156.3prime 100% Plus / Minus CG6186|FBan0006186|CT19250|FBan0006186 100% Plus / Plus *F bs29a03.y1 AI946632 singleton GH07096.5prime 100% Plus / Plus CG5395|FBan0005395|CT17122|FBan0005395 100% Plus / Plus *F bs29a04.y1 AI946633 singleton CG14872|FBan0014872|CT34691|FBan0014872 100% Plus / Plus *F bs29a06.y1 AI946634 singleton GH21371.3prime 99% Plus / Minus CG13528|FBan0013528|CT32902|FBan0013528 100% Plus / Plus *F bs29a07.y1 AI946635 singleton none none *F bs29a08.y1 AI946636 singleton GH01622.5prime 100% Plus / Plus CG3345|FBan0003345|CT36339|FBan0003345 99% Plus / Plus *F bs29a09.y1 AI946637 singleton CG18483|FBan0018483|CT42134|FBan0018483 98% Plus / Minus *F bs29a10.y1 AI946638 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs29a11.y1 AI946639 Contig430 none none *F bs29a12.y1 AI946640 singleton CG4161|FBan0004161|CT12586|FBan0004161 99% Plus / Plus *F bs29b01.y1 AI946641 Contig538 GH26445.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs29b02.y1 AI946642 Contig527 none none *F bs29b03.y1 AI946643 Contig251 CG5435|FBan0005435|CT17244|FBan0005435 99% Plus / Plus *F bs29b04.y1 AI946644 singleton CG7196|FBan0007196|CT22211|FBan0007196 99% Plus / Plus *F bs29b05.y1 AI946645 Contig428 LP09525.5prime 100% Plus / Plus CG15219|FBan0015219|CT35155|FBan0015219 100% Plus / Plus *F bs29b06.y1 AI946646 singleton LD29377.5prime 99% Plus / Plus CG7375|FBan0007375|CT22705|FBan0007375 99% Plus / Plus *F bs29b07.y1 AI946647 singleton CG7131|FBan0007131|CT22049|FBan0007131 100% Plus / Plus *F bs29b08.y1 AI946648 singleton LP03829.5prime 100% Plus / Plus CG6981|FBan0006981|CT21613|FBan0006981 100% Plus / Plus *F bs29b09.y1 AI946649 singleton none none *F bs29b10.y1 AI946650 Contig380 CG13778|FBan0013778|CT33266|FBan0013778 98% Plus / Minus *F bs29b11.y1 AI946651 Contig392 GH10365.5prime 99% Plus / Plus CG17238|FBan0017238|CT21085|FBan0017238 100% Plus / Plus *F bs29b12.y1 AI946652 Contig368 GH10330.5prime 99% Plus / Plus CG3494|FBan0003494|CT11751|FBan0003494 99% Plus / Plus *F bs29c01.y1 AI946653 Contig169 CG1690|FBan0001690|CT4772|FBan0001690 99% Plus / Plus *F bs29c02.y1 AI946654 Contig083 GH22995.5prime 96% Plus / Plus CG1979|FBan0001979|CT6207|FBan0001979 96% Plus / Plus *F bs29c03.y1 AI946655 Contig464 CG14297|FBan0014297|CT33927|FBan0014297 99% Plus / Plus *F bs29c04.y1 AI946656 Contig461 LP08687.5prime 96% Plus / Plus CG6372|FBan0006372|CT19794|FBan0006372 96% Plus / Plus *F bs29c05.y1 AI946657 Contig440 CG13030|FBan0013030|CT32248|FBan0013030 98% Plus / Plus *F bs29c06.y1 AI946658 Contig518 GH22658.5prime 97% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs29c07.y1 AI946659 Contig540 GH25305.5prime 99% Plus / Plus CG9602|FBan0009602|CT27154|FBan0009602 99% Plus / Plus *F bs29c08.y1 AI946660 Contig354 LD29004.5prime 99% Plus / Plus CG10217|FBan0010217|CT28729|FBan0010217 99% Plus / Plus *F bs29c09.y1 AI946661 Contig224 none none *F bs29c10.y1 AI946662 Contig528 GH07786.5prime 100% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 99% Plus / Plus *F bs29c12.y1 AI946663 singleton CK00453.3prime 93% Plus / Minus CG3499|FBan0003499|CT11787|FBan0003499 99% Plus / Plus *F bs29d01.y1 AI946664 singleton CG9828|FBan0009828|CT27734|FBan0009828 99% Plus / Plus *F bs29d02.y1 AI946665 singleton none none *F bs29d03.y1 pending Contig520 none none *F bs29d04.y1 AI946666 Contig411 CG11125|FBan0011125|CT8285|FBan0011125 100% Plus / Plus *F bs29d05.y1 AI946667 Contig416 LD17341.5prime 100% Plus / Plus none none *F bs29d06.y1 AI946668 singleton CG4683|FBan0004683|CT15121|FBan0004683 99% Plus / Plus *F bs29d07.y1 AI946669 Contig429 CG6380|FBan0006380|CT19908|FBan0006380 99% Plus / Plus *F bs29d08.y1 AI946670 Contig379 LD23918.3prime 99% Plus / Minus CG8073|FBan0008073|CT8072|FBan0008073 99% Plus / Plus *F bs29d09.y1 AI946671 Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs29d10.y1 AI946672 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs29d11.y1 AI946673 singleton CG14488|FBan0014488|CT34199|FBan0014488 99% Plus / Plus *F bs29d12.y1 AI946674 Contig089 none none *F bs29e01.y1 AI946675 singleton LD23670.3prime 97% Plus / Minus CG4799|FBan0004799|CT15419|FBan0004799 100% Plus / Plus *F bs29e02.y1 AI946676 singleton SD10733.5prime 98% Plus / Plus CG7740|FBan0007740|CT5671|FBan0007740 98% Plus / Plus *F bs29e03.y1 AI946677 Contig484 LP02935.5prime 100% Plus / Plus CG5538|FBan0005538|CT17502|FBan0005538 100% Plus / Plus *F bs29e04.y1 AI946678 Contig534 none none *F bs29e05.y1 AI946679 Contig424 GH24085.5prime 100% Plus / Plus CG1394|FBan0001394|CT3204|FBan0001394 100% Plus / Plus *F bs29e06.y1 AI946680 Contig536 GH19031.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 99% Plus / Plus *F bs29e07.y1 AI946681 singleton GH08978.5prime 99% Plus / Plus CG10126|FBan0010126|CT28495|FBan0010126 99% Plus / Plus *F bs29e08.y1 AI946682 singleton CG2616|FBan0002616|CT8885|FBan0002616 100% Plus / Plus *F bs29e09.y1 AI946683 Contig544 LD13779.5prime 98% Plus / Plus CG5486|FBan0005486|CT17382|FBan0005486 99% Plus / Plus *F bs29e10.y1 AI946684 Contig505 GH08240.5prime 100% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 100% Plus / Plus *F bs29e11.y1 AI946685 singleton none none *F bs29e12.y1 AI946686 Contig155 HL04954.5prime 100% Plus / Plus CG4963|FBan0004963|CT15898|FBan0004963 99% Plus / Plus *F bs29f01.y1 AI946687 singleton CG15657|FBan0015657|CT35841|FBan0015657 99% Plus / Plus *F bs29f02.y1 AI946688 Contig316 CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs29f03.y1 AI946689 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs29f04.y1 pending Contig389 none none *F bs29f05.y1 AI946690 singleton LP03094.5prime 100% Plus / Plus CG17383|FBan0017383|CT38390|FBan0017383 100% Plus / Plus *F bs29f06.y1 AI946691 singleton SD05444.5prime 99% Plus / Plus CG17767|FBan0017767|CT33834|FBan0017767 99% Plus / Plus *F bs29f07.y1 AI946692 singleton GH04494.5prime 99% Plus / Plus none none *F bs29f08.y1 AI946693 singleton CG17835|FBan0017835|CT39612|FBan0017835 97% Plus / Plus *F bs29f09.y1 AI946694 Contig495 GH10732.5prime 99% Plus / Plus CG9133|FBan0009133|CT10073|FBan0009133 100% Plus / Plus *F bs29f10.y1 AI946695 singleton CG9871|FBan0009871|CT10550|FBan0009871 97% Plus / Plus *F bs29f11.y1 AI946696 Contig485 none none *F bs29f12.y1 AI946697 Contig294 GH10618.5prime 99% Plus / Plus CG5450|FBan0005450|CT17290|FBan0005450 99% Plus / Plus *F bs29g01.y1 AI946698 singleton CG8728|FBan0008728|CT4980|FBan0008728 99% Plus / Plus *F bs29g02.y1 AI946699 Contig436 CG12250|FBan0012250|CT14866|FBan0012250 100% Plus / Plus *F bs29g03.y1 AI946700 Contig347 none none *F bs29g04.y1 AI946701 Contig544 CG7311|FBan0007311|CT22241|FBan0007311 100% Plus / Plus *F bs29g05.y1 AI946702 Contig502 GH26978.5prime 98% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 98% Plus / Plus *F bs29g06.y1 AI946703 Contig507 GH08483.5prime 99% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs29g07.y1 AI946704 singleton GH24774.5prime 97% Plus / Plus CG9528|FBan0009528|CT26960|FBan0009528 97% Plus / Plus *F bs29g08.y1 AI946705 Contig435 GH10091.3prime 100% Plus / Minus CG12562|FBan0012562|CT34305|FBan0012562 100% Plus / Plus *F bs29g09.y1 AI946706 Contig354 LD29004.5prime 99% Plus / Plus CG10217|FBan0010217|CT28729|FBan0010217 99% Plus / Plus *F bs29g10.y1 AI946707 Contig513 LP02115.5prime 99% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs29g11.y1 AI946708 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 99% Plus / Plus *F bs29g12.y1 AI946709 Contig492 GH10725.5prime 99% Plus / Plus CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs29h01.y1 AI946710 Contig369 none none *F bs29h02.y1 AI946711 Contig543 GH12543.5prime 97% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 97% Plus / Plus *F bs29h03.y1 AI946712 Contig540 none none *F bs29h04.y1 AI946713 singleton CG11929|FBan0011929|CT35791|FBan0011929 98% Plus / Plus *F bs29h05.y1 AI946714 singleton CG8840|FBan0008840|CT10939|FBan0008840 99% Plus / Plus *F bs29h06.y1 AI946715 singleton CG14864|FBan0014864|CT34683|FBan0014864 100% Plus / Plus *F bs29h07.y1 AI946716 singleton GM10356.5prime 99% Plus / Plus CG14615|FBan0014615|CT34372|FBan0014615 98% Plus / Plus *F bs29h08.y1 AI946717 singleton LD20554.5prime 100% Plus / Plus CG18218|FBan0018218|CT41222|FBan0018218 99% Plus / Plus *F bs29h09.y1 pending Contig354 CK01633.contig 99% Plus / Plus CG2830|FBan0002830|CT9652|FBan0002830 99% Plus / Plus *F bs29h10.y1 AI946718 singleton HL01520.5prime 98% Plus / Minus CG17927|FBan0017927|CT39920|FBan0017927 98% Plus / Plus *F bs29h11.y1 AI946719 singleton CG5388|FBan0005388|CT17106|FBan0005388 99% Plus / Plus *F bs29h12.y1 AI946720 Contig109 LD15307.5prime 100% Plus / Plus CG8104|FBan0008104|CT24240|FBan0008104 100% Plus / Plus *F bs30a01.y1 AI946721 Contig505 none none *F bs30a02.y1 AI946722 singleton LP12271.5prime 100% Plus / Plus CG7939|FBan0007939|CT6405|FBan0007939 100% Plus / Plus *F bs30a03.y1 AI946723 Contig299 CG6262|FBan0006262|CT42653|FBan0006262 99% Plus / Plus *F bs30a04.y1 AI946724 singleton CG10718|FBan0010718|CT30035|FBan0010718 99% Plus / Plus *F bs30a05.y1 AI946725 Contig442 CG5024|FBan0005024|CT16147|FBan0005024 97% Plus / Plus *F bs30a06.y1 pending Contig192 LP08562.5prime 95% Plus / Plus CG4463|FBan0004463|CT14526|FBan0004463 95% Plus / Plus *F bs30a07.y1 AI946726 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 98% Plus / Plus *F bs30a08.y1 AI946727 Contig503 GH11521.5prime 100% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 100% Plus / Plus *F bs30a09.y1 AI946728 singleton none none *F bs30a10.y1 AI946729 singleton CG6209|FBan0006209|CT19464|FBan0006209 99% Plus / Plus *F bs30a11.y1 AI946730 Contig104 GH14476.5prime 99% Plus / Plus CG3565|FBan0003565|CT11950|FBan0003565 99% Plus / Plus *F bs30a12.y1 AI946731 Contig332 CG6497|FBan0006497|CT20241|FBan0006497 99% Plus / Plus *F bs30b01.y1 AI946732 Contig543 GH11908.5prime 99% Plus / Minus CG9284|FBan0009284|CT26453|FBan0009284 99% Plus / Minus *F bs30b02.y1 AI946733 singleton CG11288|FBan0011288|CT31491|FBan0011288 99% Plus / Plus *F bs30b03.y1 AI946734 Contig339 LP05950.5prime 99% Plus / Plus CG8278|FBan0008278|CT7966|FBan0008278 99% Plus / Plus *F bs30b04.y1 AI946735 singleton SD06914.5prime 100% Plus / Plus CG6006|FBan0006006|CT18835|FBan0006006 100% Plus / Plus *F bs30b05.y1 AI946736 Contig539 GH27943.3prime 97% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs30b06.y1 AI946737 Contig525 LD07329.5prime 97% Plus / Plus CG8983|FBan0008983|CT25820|FBan0008983 99% Plus / Plus *F bs30b07.y1 AI946738 Contig513 LP02115.5prime 99% Plus / Plus CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs30b08.y1 AI946739 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs30b09.y1 AI946740 singleton GH16555.5prime 98% Plus / Plus CG4827|FBan0004827|CT15509|FBan0004827 100% Plus / Plus *F bs30b10.y1 AI946741 Contig542 GH28631.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 99% Plus / Plus *F bs30b11.y1 AI946742 singleton CG11773|FBan0011773|CT36855|FBan0011773 99% Plus / Plus *F bs30b12.y1 AI946743 Contig355 LD24411.5prime 99% Plus / Plus CG8284|FBan0008284|CT24417|FBan0008284 99% Plus / Plus *F bs30c01.y1 AI946744 Contig520 none none *F bs30c02.y1 AI946745 Contig377 LP04712.5prime 99% Plus / Plus none none *F bs30c03.y1 AI946746 Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs30c04.y1 AI946747 singleton CG11428|FBan0011428|CT31897|FBan0011428 98% Plus / Plus *F bs30c05.y1 AI946748 Contig445 LP10010.5prime 94% Plus / Plus CG1288|FBan0001288|CT2110|FBan0001288 98% Plus / Plus *F bs30c06.y1 AI946749 singleton CG5253|FBan0005253|CT16785|FBan0005253 99% Plus / Plus *F bs30c07.y1 AI946750 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs30c08.y1 AI946751 singleton CG8710|FBan0008710|CT9449|FBan0008710 99% Plus / Plus *F bs30c09.y1 AI946752 singleton CG1722|FBan0001722|CT4924|FBan0001722 97% Plus / Plus *F bs30c10.y1 AI946753 singleton CG2750|FBan0002750|CT9367|FBan0002750 99% Plus / Plus *F bs30c11.y1 AI946754 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs30c12.y1 AI946755 singleton GH07247.5prime 100% Plus / Plus CG3610|FBan0003610|CT12129|FBan0003610 100% Plus / Plus *F bs30d01.y1 AI946756 singleton LP10926.5prime 99% Plus / Plus CG7176|FBan0007176|CT22171|FBan0007176 100% Plus / Plus *F bs30d02.y1 AI946757 Contig418 LP08248.5prime 100% Plus / Plus CG10578|FBan0010578|CT29670|FBan0010578 100% Plus / Plus *F bs30d03.y1 AI946758 singleton CG18132|FBan0018132|CT40836|FBan0018132 99% Plus / Plus *F bs30d04.y1 AI946759 singleton SD07206.5prime 99% Plus / Plus CG8151|FBan0008151|CT24236|FBan0008151 99% Plus / Plus *F bs30d05.y1 AI946760 Contig433 LP01014.3prime 98% Plus / Minus CG4265|FBan0004265|CT10917|FBan0004265 98% Plus / Plus *F bs30d06.y1 AI946761 Contig353 CK02399.contig 99% Plus / Plus CG11419|FBan0011419|CT31877|FBan0011419 99% Plus / Plus *F bs30d07.y1 AI946762 singleton GH26914.5prime 100% Plus / Plus CG9074|FBan0009074|CT26012|FBan0009074 100% Plus / Plus *F bs30d08.y1 AI946763 singleton none none *F bs30d09.y1 AI946764 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs30d10.y1 AI946765 singleton GM04146.5prime 98% Plus / Plus CG7340|FBan0007340|CT42368|FBan0007340 99% Plus / Plus *F bs30d11.y1 pending Contig311 none none *F bs30d12.y1 AI946766 singleton CG12395|FBan0012395|CT26316|FBan0012395 99% Plus / Plus *F bs30e01.y1 AI946767 singleton LP03267.5prime 97% Plus / Plus CG5454|FBan0005454|CT16984|FBan0005454 98% Plus / Plus *F bs30e02.y1 AI946768 Contig462 CG18427|FBan0018427|CT30112|FBan0018427 100% Plus / Plus *F bs30e03.y1 AI946769 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs30e04.y1 AI946770 singleton GH15825.5prime 99% Plus / Plus CG3085|FBan0003085|CT10370|FBan0003085 99% Plus / Plus *F bs30e05.y1 AI946771 singleton CG3611|FBan0003611|CT12131|FBan0003611 99% Plus / Plus *F bs30e06.y1 AI946772 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs30e07.y1 AI946773 Contig489 GH10553.5prime 99% Plus / Plus CG5045|FBan0005045|CT16189|FBan0005045 99% Plus / Plus *F bs30e08.y1 AI946774 Contig182 GH07940.5prime 98% Plus / Plus CG11018|FBan0011018|CT30849|FBan0011018 98% Plus / Plus *F bs30e09.y1 AI946775 Contig272 none none *F bs30e10.y1 AI946776 Contig521 GH21541.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs30e11.y1 AI946777 Contig342 CG5504|FBan0005504|CT17450|FBan0005504 98% Plus / Plus *F bs30e12.y1 AI946778 Contig470 GH25094.5prime 100% Plus / Plus CG17450|FBan0017450|CT34184|FBan0017450 100% Plus / Plus *F bs30f01.y1 AI946779 singleton CG1472|FBan0001472|CT3600|FBan0001472 99% Plus / Plus *F bs30f02.y1 AI946780 Contig475 LP02384.5prime 100% Plus / Plus CG7931|FBan0007931|CT6483|FBan0007931 98% Plus / Plus *F bs30f03.y1 AI946781 Contig479 CG5735|FBan0005735|CT18021|FBan0005735 100% Plus / Plus *F bs30f04.y1 AI946782 Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs30f05.y1 AI946783 singleton none none *F bs30f06.y1 AI946784 Contig518 GH25878.5prime 97% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs30f07.y1 AI946785 Contig490 LP05614.5prime 99% Plus / Plus CG13263|FBan0013263|CT32547|FBan0013263 100% Plus / Plus *F bs30f08.y1 AI946786 Contig341 GH19562.5prime 99% Plus / Plus CG6412|FBan0006412|CT19988|FBan0006412 99% Plus / Plus *F bs30f09.y1 AI946787 singleton CG11068|FBan0011068|CT30965|FBan0011068 100% Plus / Plus *F bs30f10.y1 AI946788 singleton LP08864.5prime 98% Plus / Plus CG1138|FBan0001138|CT1910|FBan0001138 98% Plus / Plus *F bs30f11.y1 AI946789 singleton GH04392.5prime 94% Plus / Plus CG12402|FBan0012402|CT27922|FBan0012402 96% Plus / Plus *F bs30f12.y1 AI946790 singleton GM03566.5prime 99% Plus / Plus CG8048|FBan0008048|CT42084|FBan0008048 99% Plus / Plus *F bs30g01.y1 AI946791 Contig191 CG13243|FBan0013243|CT32493|FBan0013243 99% Plus / Minus *F bs30g02.y1 AI946792 Contig515 GH06743.5prime 100% Plus / Minus none none *F bs30g03.y1 AI946793 Contig452 CG3085|FBan0003085|CT10370|FBan0003085 100% Plus / Plus *F bs30g04.y1 AI946794 singleton GH04550.5prime 99% Plus / Plus CG15445|FBan0015445|CT35509|FBan0015445 100% Plus / Plus *F bs30g05.y1 AI946795 Contig343 CG18370|FBan0018370|CT41755|FBan0018370 97% Plus / Plus *F bs30g06.y1 AI946796 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs30g07.y1 AI946797 Contig424 GH14622.5prime 100% Plus / Plus CG1394|FBan0001394|CT3204|FBan0001394 100% Plus / Plus *F bs30g08.y1 AI946798 Contig464 CG14297|FBan0014297|CT33927|FBan0014297 99% Plus / Plus *F bs30g09.y1 AI946799 Contig202 LP10575.3prime 99% Plus / Plus none none *F bs30g10.y1 AI946800 Contig474 GH11908.5prime 98% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 98% Plus / Plus *F bs30g11.y1 AI946801 Contig300 CG9592|FBan0009592|CT27112|FBan0009592 99% Plus / Plus *F bs30g12.y1 AI946802 singleton LD28483.5prime 98% Plus / Plus CG4502|FBan0004502|CT14647|FBan0004502 98% Plus / Plus *F bs30h01.y1 AI946803 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs30h02.y1 AI946804 Contig211 CG1287|FBan0001287|CT2727|FBan0001287 99% Plus / Plus *F bs30h03.y1 AI946805 Contig530 GH13953.5prime 99% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs30h04.y1 AI946806 Contig430 none none *F bs30h05.y1 AI946807 Contig544 GH20547.5prime 99% Plus / Plus none none *F bs30h06.y1 AI946808 singleton LD28511.5prime 99% Plus / Minus CG2702|FBan0002702|CT9219|FBan0002702 99% Plus / Minus *F bs30h07.y1 AI946809 Contig212 LP09983.5prime 99% Plus / Plus CG5885|FBan0005885|CT18469|FBan0005885 99% Plus / Plus *F bs30h08.y1 AI946810 Contig339 LP05950.5prime 100% Plus / Plus CG8278|FBan0008278|CT7966|FBan0008278 100% Plus / Plus *F bs30h09.y1 AI946811 singleton LD23817.3prime 98% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs30h10.y1 AI946812 Contig508 LD23817.3prime 98% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs30h11.y1 AI946813 singleton LD29841.5prime 99% Plus / Plus CG6343|FBan0006343|CT19828|FBan0006343 100% Plus / Plus *F bs30h12.y1 AI946814 Contig501 GH20739.5prime 100% Plus / Plus CG9975|FBan0009975|CT28093|FBan0009975 98% Plus / Plus *F bs31a03.y1 AI946815 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs31a05.y1 pending singleton HL05941.5prime 99% Plus / Plus CG2950|FBan0002950|CT9989|FBan0002950 99% Plus / Plus *F bs31a06.y1 pending singleton none none *F bs31a07.y1 AI946816 Contig505 GH02250.5prime 100% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 100% Plus / Plus *F bs31a08.y1 pending Contig338 GM01842.5prime 100% Plus / Plus CG10691|FBan0010691|CT29956|FBan0010691 98% Plus / Plus *F bs31a10.y1 pending singleton none none *F bs31a11.y1 AI946817 Contig539 LD21467.5prime 100% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs31a12.y1 AI946818 singleton CG14690|FBan0014690|CT34476|FBan0014690 100% Plus / Plus *F bs31b02.y1 AI946819 Contig542 GH22924.5prime 100% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs31b03.y1 AI946820 singleton CG8335|FBan0008335|CT4916|FBan0008335 99% Plus / Plus *F bs31b04.y1 AI946821 Contig323 LD32260.5prime 98% Plus / Plus CG9288|FBan0009288|CT26469|FBan0009288 100% Plus / Plus *F bs31b08.y1 AI946822 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs31b10.y1 AI946823 Contig544 GH22881.5prime 98% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 98% Plus / Plus *F bs31b11.y1 pending Contig376 GH20473.5prime 100% Plus / Plus none none *F bs31c03.y1 AI946824 Contig539 CG2127|FBan0002127|CT6944|FBan0002127 100% Plus / Plus *F bs31c04.y1 AI946825 Contig326 LP11903.5prime 100% Plus / Plus CG1534|FBan0001534|CT3947|FBan0001534 100% Plus / Plus *F bs31c05.y1 AI946826 Contig543 GH11908.3prime 94% Plus / Plus CG9284|FBan0009284|CT26453|FBan0009284 94% Plus / Minus *F bs31c06.y1 AI946827 singleton CG11591|FBan0011591|CT36552|FBan0011591 100% Plus / Plus *F bs31c07.y1 pending Contig540 none none *F bs31c08.y1 AI946828 Contig522 LP09246.5prime 99% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 100% Plus / Plus *F bs31c10.y1 AI946829 singleton SD03463.5prime 100% Plus / Plus CG17273|FBan0017273|CT35906|FBan0017273 100% Plus / Plus *F bs31c11.y1 AI946830 Contig505 GH02250.5prime 100% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 100% Plus / Plus *F bs31c12.y1 AI946831 Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs31d04.y1 AI946832 Contig143 CG15425|FBan0015425|CT35487|FBan0015425 100% Plus / Plus *F bs31d06.y1 pending Contig525 none none *F bs31d07.y1 pending singleton CG18368|FBan0018368|CT41747|FBan0018368 99% Plus / Plus *F bs31d08.y1 AI946833 Contig486 CG7268|FBan0007268|CT22431|FBan0007268 98% Plus / Plus *F bs31d10.y1 pending Contig528 GH13107.5prime 96% Plus / Plus CG5089|FBan0005089|CT16301|FBan0005089 96% Plus / Plus *F bs31d11.y1 pending singleton CG17945|FBan0017945|CT39980|FBan0017945 100% Plus / Plus *F bs31d12.y1 pending singleton LD46744.5prime 99% Plus / Plus CG9878|FBan0009878|CT27866|FBan0009878 100% Plus / Plus *F bs31e02.y1 pending Contig269 CG15086|FBan0015086|CT34961|FBan0015086 98% Plus / Plus *F bs31e03.y1 AI946834 Contig441 GH26265.5prime 100% Plus / Plus none none *F bs31e04.y1 AI946835 Contig366 CK01909.contig 94% Plus / Plus CG6756|FBan0006756|CT20965|FBan0006756 100% Plus / Plus *F bs31e06.y1 pending singleton GH28790.5prime 93% Plus / Plus none none *F bs31e07.y1 pending Contig495 GH25207.5prime 99% Plus / Plus CG9133|FBan0009133|CT10073|FBan0009133 99% Plus / Plus *F bs31e09.y1 pending Contig314 CG11379|FBan0011379|CT31766|FBan0011379 100% Plus / Plus *F bs31e12.y1 pending Contig495 GH19464.3prime 99% Plus / Minus CG9133|FBan0009133|CT10073|FBan0009133 99% Plus / Plus *F bs31f03.y1 AI946836 Contig503 GH11521.5prime 99% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 99% Plus / Plus *F bs31f04.y1 AI946837 singleton CG15703|FBan0015703|CT35924|FBan0015703 99% Plus / Plus *F bs31f05.y1 AI946838 Contig505 GH02250.5prime 99% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 99% Plus / Plus *F bs31f06.y1 pending singleton CG1638|FBan0001638|CT4356|FBan0001638 99% Plus / Plus *F bs31f09.y1 AI946839 Contig135 CG7969|FBan0007969|CT23844|FBan0007969 99% Plus / Plus *F bs31f10.y1 AI946840 Contig504 GH13129.3prime 100% Plus / Minus CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs31f12.y1 AI946841 Contig357 SD02767.5prime 100% Plus / Plus CG13176|FBan0013176|CT32417|FBan0013176 99% Plus / Plus *F bs31g01.y1 AI946842 singleton CG8065|FBan0008065|CT24188|FBan0008065 98% Plus / Plus *F bs31g02.y1 AI946843 Contig312 GH02649.5prime 100% Plus / Plus CG7366|FBan0007366|CT22689|FBan0007366 100% Plus / Plus *F bs31g03.y1 AI946844 singleton CG15034|FBan0015034|CT34897|FBan0015034 100% Plus / Plus *F bs31g04.y1 AI946845 singleton SD01750.5prime 99% Plus / Plus CG8605|FBan0008605|CT15199|FBan0008605 99% Plus / Plus *F bs31g05.y1 AI946846 Contig362 CG13747|FBan0013747|CT33223|FBan0013747 100% Plus / Plus *F bs31g06.y1 AI946847 Contig313 LD38824.5prime 100% Plus / Plus CG4994|FBan0004994|CT15958|FBan0004994 100% Plus / Plus *F bs31g08.y1 AI946848 Contig352 CG12313|FBan0012313|CT21286|FBan0012313 100% Plus / Plus *F bs31g09.y1 pending singleton GH05133.5prime 100% Plus / Plus CG6169|FBan0006169|CT19348|FBan0006169 100% Plus / Plus *F bs31g10.y1 AI946849 Contig287 GH05818.5prime 99% Plus / Plus CG17666|FBan0017666|CT39001|FBan0017666 99% Plus / Plus *F bs31g11.y1 pending singleton none none *F bs31g12.y1 AI946850 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs31h01.y1 AI946851 Contig371 CG14305|FBan0014305|CT33935|FBan0014305 99% Plus / Plus *F bs31h02.y1 AI946852 Contig449 CG12201|FBan0012201|CT10663|FBan0012201 99% Plus / Plus *F bs31h03.y1 AI946853 Contig537 GH07855.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs31h04.y1 AI946854 singleton LP03110.5prime 99% Plus / Plus CG6058|FBan0006058|CT41919|FBan0006058 100% Plus / Plus *F bs31h06.y1 AI946855 Contig347 none none *F bs31h08.y1 AI946856 Contig480 GH15272.5prime 100% Plus / Plus CG1999|FBan0001999|CT6363|FBan0001999 100% Plus / Plus *F bs31h10.y1 AI946857 Contig476 LP11595.5prime 97% Plus / Plus CG3752|FBan0003752|CT12541|FBan0003752 99% Plus / Plus *F bs31h11.y1 pending singleton GH15153.5prime 98% Plus / Plus CG14616|FBan0014616|CT34373|FBan0014616 98% Plus / Plus *F bs31h12.y1 AI946858 Contig314 CG11379|FBan0011379|CT31766|FBan0011379 100% Plus / Plus *F bs32a03.y1 AI946859 singleton GH02930.5prime 98% Plus / Plus CG17648|FBan0017648|CT32529|FBan0017648 98% Plus / Plus *F bs32a04.y1 pending singleton LD13794.5prime 98% Plus / Plus CG4293|FBan0004293|CT41525|FBan0004293 99% Plus / Plus *F bs32a05.y1 AI946860 singleton GH24307.5prime 98% Plus / Plus CG12203|FBan0012203|CT10825|FBan0012203 98% Plus / Plus *F bs32a06.y1 AI946861 Contig538 GH26445.5prime 97% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 97% Plus / Plus *F bs32a07.y1 pending Contig512 LP07747.5prime 98% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 98% Plus / Plus *F bs32a08.y1 AI946862 singleton CG4546|FBan0004546|CT14712|FBan0004546 100% Plus / Plus *F bs32a09.y1 AI946863 Contig495 GH25260.5prime 99% Plus / Plus CG9133|FBan0009133|CT10073|FBan0009133 99% Plus / Plus *F bs32a11.y1 pending singleton none none *F bs32a12.y1 AI946864 singleton GH17673.5prime 100% Plus / Plus CG8415|FBan0008415|CT24703|FBan0008415 100% Plus / Plus *F bs32b01.y1 pending singleton CK01371.contig 97% Plus / Plus CG1418|FBan0001418|CT3354|FBan0001418 97% Plus / Plus *F bs32b02.y1 AI946865 Contig418 LP01757.5prime 100% Plus / Plus CG10578|FBan0010578|CT29670|FBan0010578 100% Plus / Plus *F bs32b03.y1 AI946866 Contig507 GM04405.5prime 99% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs32b04.y1 AI946867 Contig310 CG9406|FBan0009406|CT26694|FBan0009406 99% Plus / Plus *F bs32b05.y1 AI946868 Contig523 LD23336.3prime 99% Plus / Minus CG7061|FBan0007061|CT37683|FBan0007061 99% Plus / Plus *F bs32b06.y1 AI946869 singleton CG8236|FBan0008236|CT24457|FBan0008236 100% Plus / Plus *F bs32b07.y1 pending Contig286 GH25284.5prime 93% Plus / Plus CG5207|FBan0005207|CT16475|FBan0005207 96% Plus / Plus *F bs32b08.y1 AI946870 singleton none none *F bs32b09.y1 AI946871 Contig450 GH23475.5prime 100% Plus / Plus none none *F bs32b10.y1 AI946872 singleton LD40842.5prime 99% Plus / Plus CG4878|FBan0004878|CT15671|FBan0004878 99% Plus / Plus *F bs32b11.y1 AI946873 Contig311 CG14013|FBan0014013|CT33570|FBan0014013 99% Plus / Plus *F bs32b12.y1 pending Contig444 GH23207.5prime 95% Plus / Plus CG2922|FBan0002922|CT7240|FBan0002922 96% Plus / Plus *F bs32c02.y1 AI946874 singleton LD39082.5prime 98% Plus / Plus CG10639|FBan0010639|CT29796|FBan0010639 99% Plus / Plus *F bs32c03.y1 AI946875 Contig538 GH10186.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs32c04.y1 AI946876 Contig496 CK00537.5prime 99% Plus / Plus CG7441|FBan0007441|CT22887|FBan0007441 100% Plus / Plus *F bs32c05.y1 AI946877 Contig512 LP07747.5prime 98% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 98% Plus / Plus *F bs32c06.y1 AI946878 singleton CG7474|FBan0007474|CT8014|FBan0007474 100% Plus / Plus *F bs32c07.y1 AI946879 singleton HL01034.5prime 98% Plus / Plus CG15630|FBan0015630|CT35785|FBan0015630 98% Plus / Plus *F bs32c10.y1 AI946880 Contig362 CG13747|FBan0013747|CT33223|FBan0013747 100% Plus / Plus *F bs32c12.y1 AI946881 Contig128 CG11298|FBan0011298|CT31529|FBan0011298 98% Plus / Plus *F bs32d05.y1 AI946882 Contig213 LP04516.5prime 100% Plus / Plus CG4046|FBan0004046|CT13148|FBan0004046 100% Plus / Plus *F bs32d06.y1 AI946883 Contig486 CG7268|FBan0007268|CT22431|FBan0007268 99% Plus / Plus *F bs32d07.y1 AI946884 Contig529 GH13129.3prime 94% Plus / Minus CG6372|FBan0006372|CT19736|FBan0006372 100% Plus / Plus *F bs32d08.y1 pending Contig340 GH01027.5prime 99% Plus / Plus CG10124|FBan0010124|CT28485|FBan0010124 99% Plus / Plus *F bs32d10.y1 AI946885 Contig544 CG12377|FBan0012377|CT25244|FBan0012377 100% Plus / Plus *F bs32d11.y1 pending Contig509 GM13259.5prime 95% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 95% Plus / Plus *F bs32d12.y1 AI946886 singleton CG7177|FBan0007177|CT22173|FBan0007177 99% Plus / Plus *F bs32e03.y1 AI946887 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs32e04.y1 pending Contig440 GH28479.5prime 100% Plus / Plus CG13030|FBan0013030|CT32248|FBan0013030 100% Plus / Plus *F bs32e05.y1 AI946888 Contig514 GH24664.5prime 100% Plus / Plus CG17784|FBan0017784|CT33005|FBan0017784 100% Plus / Minus *F bs32e06.y1 AI946889 singleton CG6025|FBan0006025|CT18906|FBan0006025 100% Plus / Plus *F bs32e08.y1 AI946890 singleton LP06471.5prime 100% Plus / Plus CG1971|FBan0001971|CT6195|FBan0001971 100% Plus / Plus *F bs32e09.y1 AI946891 Contig540 none none *F bs32e10.y1 pending Contig540 none none *F bs32e11.y1 AI946892 Contig361 GH27175.5prime 96% Plus / Plus CG4479|FBan0004479|CT14582|FBan0004479 99% Plus / Plus *F bs32f01.y1 pending Contig385 GH04024.5prime 95% Plus / Plus CG8023|FBan0008023|CT24088|FBan0008023 95% Plus / Plus *F bs32f03.y1 pending Contig385 GH04024.5prime 94% Plus / Plus CG8023|FBan0008023|CT24088|FBan0008023 94% Plus / Plus *F bs32f04.y1 pending Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs32f05.y1 AI946893 singleton CG10367|FBan0010367|CT29116|FBan0010367 99% Plus / Plus *F bs32f06.y1 AI946894 Contig350 CG17838|FBan0017838|CT39628|FBan0017838 100% Plus / Plus *F bs32f07.y1 AI946895 Contig317 none none *F bs32f08.y1 pending Contig229 LD45146.5prime 98% Plus / Plus CG7007|FBan0007007|CT21672|FBan0007007 98% Plus / Plus *F bs32f09.y1 AI946896 singleton LD30192.5prime 98% Plus / Plus CG7453|FBan0007453|CT22925|FBan0007453 98% Plus / Plus *F bs32f10.y1 AI946897 Contig512 LP07747.5prime 98% Plus / Plus CG7363|FBan0007363|CT22661|FBan0007363 98% Plus / Plus *F bs32f11.y1 AI946898 Contig498 GH22658.5prime 98% Plus / Plus CG18461|FBan0018461|CT38382|FBan0018461 97% Plus / Plus *F bs32f12.y1 AI946899 Contig325 CG11327|FBan0011327|CT31615|FBan0011327 99% Plus / Plus *F bs32g01.y1 pending singleton CG18368|FBan0018368|CT41747|FBan0018368 96% Plus / Plus *F bs32g02.y1 AI946900 singleton GM14163.5prime 85% Plus / Plus CG11423|FBan0011423|CT31889|FBan0011423 100% Plus / Plus *F bs32g04.y1 AI946901 Contig544 LP06279.5prime 98% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 98% Plus / Plus *F bs32g05.y1 AI946902 Contig505 GH08240.5prime 100% Plus / Plus CG7929|FBan0007929|CT6423|FBan0007929 100% Plus / Plus *F bs32g06.y1 AI946903 singleton LP06769.5prime 98% Plus / Plus CG5609|FBan0005609|CT17726|FBan0005609 98% Plus / Plus *F bs32g07.y1 AI946904 Contig131 GH18529.5prime 99% Plus / Plus CG8493|FBan0008493|CT24841|FBan0008493 100% Plus / Plus *F bs32g08.y1 AI946905 singleton GH26103.5prime 100% Plus / Plus CG14648|FBan0014648|CT34420|FBan0014648 100% Plus / Plus *F bs32g09.y1 AI946906 Contig324 CG8476|FBan0008476|CT24775|FBan0008476 99% Plus / Plus *F bs32g10.y1 AI946907 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs32g11.y1 pending Contig110 CG3575|FBan0003575|CT12015|FBan0003575 100% Plus / Plus *F bs32g12.y1 AI946908 singleton SD02683.5prime 98% Plus / Plus CG4153|FBan0004153|CT13682|FBan0004153 100% Plus / Plus *F bs32h02.y1 pending singleton LD47847.5prime 100% Plus / Plus CG1591|FBan0001591|CT4191|FBan0001591 100% Plus / Plus *F bs32h03.y1 pending Contig499 CG18184|FBan0018184|CT41084|FBan0018184 91% Plus / Plus *F bs32h04.y1 pending Contig427 CG18131|FBan0018131|CT40834|FBan0018131 96% Plus / Plus *F bs32h05.y1 AI946909 Contig465 CG4434|FBan0004434|CT14426|FBan0004434 99% Plus / Plus *F bs32h06.y1 AI946910 singleton CG14735|FBan0014735|CT34528|FBan0014735 100% Plus / Plus *F bs32h07.y1 AI946911 singleton GH15245.5prime 100% Plus / Plus CG17769|FBan0017769|CT39380|FBan0017769 100% Plus / Plus *F bs32h08.y1 AI946912 singleton none none *F bs32h09.y1 pending singleton GH18869.5prime 89% Plus / Plus CG4535|FBan0004535|CT14666|FBan0004535 89% Plus / Plus *F bs32h10.y1 AI946913 singleton LD47401.5prime 100% Plus / Plus CG10306|FBan0010306|CT28939|FBan0010306 96% Plus / Plus *F bs32h11.y1 pending singleton GH10864.5prime 96% Plus / Plus CG2171|FBan0002171|CT6334|FBan0002171 96% Plus / Plus *F bs32h12.y1 pending Contig525 none none *F bs33a01.y1 AI946914 Contig351 GH16413.5prime 98% Plus / Plus CG4767|FBan0004767|CT15347|FBan0004767 99% Plus / Plus *F bs33a02.y1 AI946915 singleton CG8998|FBan0008998|CT25866|FBan0008998 100% Plus / Plus *F bs33a03.y1 AI946916 singleton CG2577|FBan0002577|CT8733|FBan0002577 99% Plus / Plus *F bs33a04.y1 pending Contig309 none none *F bs33a05.y1 AI946917 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs33a06.y1 AI946918 Contig367 none none *F bs33a07.y1 AI946919 Contig309 none none *F bs33a08.y1 AI946920 singleton GH05342.5prime 99% Plus / Plus CG10999|FBan0010999|CT30805|FBan0010999 99% Plus / Plus *F bs33a09.y1 AI946921 Contig542 GH22924.5prime 98% Plus / Plus CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs33a10.y1 AI946922 Contig539 GH27943.3prime 99% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs33a11.y1 AI946923 Contig472 GH19274.5prime 88% Plus / Plus CG9129|FBan0009129|CT8135|FBan0009129 99% Plus / Plus *F bs33a12.y1 AI946924 Contig342 CG5504|FBan0005504|CT17450|FBan0005504 99% Plus / Plus *F bs33b01.y1 AI946925 Contig532 GH16267.5prime 97% Plus / Plus CG17349|FBan0017349|CT32310|FBan0017349 98% Plus / Plus *F bs33b02.y1 AI946926 Contig539 GH27943.3prime 98% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs33b03.y1 AI946927 Contig496 GH09538.5prime 100% Plus / Plus CG7441|FBan0007441|CT22887|FBan0007441 100% Plus / Plus *F bs33b04.y1 AI946928 Contig529 LP04643.5prime 99% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 97% Plus / Plus *F bs33b05.y1 AI946929 singleton CG18416|FBan0018416|CT41902|FBan0018416 99% Plus / Plus *F bs33b06.y1 AI946930 Contig116 GH10882.5prime 98% Plus / Plus CG17564|FBan0017564|CT38791|FBan0017564 99% Plus / Plus *F bs33b07.y1 AI946931 Contig180 LD42191.5prime 98% Plus / Plus CG5514|FBan0005514|CT17470|FBan0005514 99% Plus / Plus *F bs33b08.y1 AI946932 Contig095 LP10258.5prime 99% Plus / Plus CG8251|FBan0008251|CT2835|FBan0008251 99% Plus / Plus *F bs33b09.y1 AI946933 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs33b10.y1 AI946934 singleton GH13231.5prime 98% Plus / Plus CG3713|FBan0003713|CT12463|FBan0003713 98% Plus / Plus *F bs33b11.y1 AI946935 singleton SD04823.5prime 98% Plus / Plus CG1906|FBan0001906|CT2831|FBan0001906 100% Plus / Plus *F bs33b12.y1 AI946936 Contig399 GH07182.5prime 97% Plus / Plus CG5217|FBan0005217|CT16693|FBan0005217 97% Plus / Plus *F bs33c01.y1 AI946937 singleton LP09071.5prime 99% Plus / Plus CG15211|FBan0015211|CT35143|FBan0015211 99% Plus / Plus *F bs33c02.y1 AI946938 Contig132 LD28515.5prime 100% Plus / Plus CG6701|FBan0006701|CT20818|FBan0006701 99% Plus / Plus *F bs33c03.y1 AI946939 Contig480 GH15272.5prime 99% Plus / Plus CG1999|FBan0001999|CT6363|FBan0001999 100% Plus / Plus *F bs33c04.y1 AI946940 Contig424 GH14622.5prime 100% Plus / Plus CG1394|FBan0001394|CT3204|FBan0001394 100% Plus / Plus *F bs33c05.y1 AI946941 Contig335 GH28778.5prime 99% Plus / Plus CG4692|FBan0004692|CT15135|FBan0004692 99% Plus / Plus *F bs33c06.y1 AI946942 singleton CG5950|FBan0005950|CT18686|FBan0005950 99% Plus / Plus *F bs33c07.y1 AI946943 singleton CG10920|FBan0010920|CT30589|FBan0010920 100% Plus / Plus *F bs33c08.y1 AI946944 singleton GH19032.5prime 98% Plus / Plus CG8701|FBan0008701|CT7042|FBan0008701 98% Plus / Plus *F bs33c09.y1 AI946945 Contig174 CG18463|FBan0018463|CT33393|FBan0018463 98% Plus / Plus *F bs33c10.y1 AI946946 singleton CG16972|FBan0016972|CT35579|FBan0016972 99% Plus / Plus *F bs33c11.y1 AI946947 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs33c12.y1 AI946948 singleton LD32726.5prime 99% Plus / Plus CG8295|FBan0008295|CT21621|FBan0008295 99% Plus / Plus *F bs33d01.y1 AI946949 Contig531 CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs33d02.y1 AI946950 singleton CG15086|FBan0015086|CT34961|FBan0015086 98% Plus / Plus *F bs33d03.y1 AI946951 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 99% Plus / Plus *F bs33d04.y1 AI946952 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 98% Plus / Plus *F bs33d06.y1 AI946953 singleton LD30164.5prime 99% Plus / Plus CG11981|FBan0011981|CT32122|FBan0011981 99% Plus / Plus *F bs33d07.y1 AI946954 Contig539 GH27943.3prime 98% Plus / Minus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs33d08.y1 AI946955 Contig092 none none *F bs33d09.y1 AI946956 Contig294 GH10618.5prime 99% Plus / Plus CG5450|FBan0005450|CT17290|FBan0005450 99% Plus / Plus *F bs33d10.y1 AI946957 Contig525 GH11896.5prime 98% Plus / Plus CG10219|FBan0010219|CT28735|FBan0010219 98% Plus / Plus *F bs33d11.y1 AI946958 Contig221 GH13434.5prime 99% Plus / Plus CG10869|FBan0010869|CT30427|FBan0010869 99% Plus / Plus *F bs33d12.y1 AI946959 Contig519 CG6275|FBan0006275|CT19618|FBan0006275 98% Plus / Plus *F bs33e02.y1 AI946960 Contig165 GH18088.5prime 100% Plus / Plus none none *F bs33e03.y1 AI946961 Contig339 LP05950.5prime 100% Plus / Plus CG8278|FBan0008278|CT7966|FBan0008278 100% Plus / Plus *F bs33e04.y1 pending singleton CG10984|FBan0010984|CT30777|FBan0010984 98% Plus / Plus *F bs33e05.y1 AI946962 Contig088 CG7309|FBan0007309|CT22551|FBan0007309 100% Plus / Plus *F bs33e06.y1 AI946963 Contig537 GH07855.3prime 98% Plus / Minus CG8701|FBan0008701|CT7042|FBan0008701 99% Plus / Plus *F bs33e07.y1 AI946964 singleton CG5493|FBan0005493|CT17422|FBan0005493 98% Plus / Minus *F bs33e08.y1 AI946965 Contig353 GH07784.5prime 100% Plus / Plus CG10045|FBan0010045|CT28269|FBan0010045 100% Plus / Plus *F bs33e09.y1 AI946966 singleton CG8349|FBan0008349|CT24559|FBan0008349 99% Plus / Plus *F bs33e10.y1 AI946967 Contig544 CG11358|FBan0011358|CT31682|FBan0011358 100% Plus / Plus *F bs33e11.y1 AI946968 Contig404 GH13594.3prime 99% Plus / Minus CG14718|FBan0014718|CT34509|FBan0014718 99% Plus / Plus *F bs33e12.y1 AI946969 Contig096 CG5662|FBan0005662|CT17888|FBan0005662 99% Plus / Plus *F bs33f01.y1 AI946970 Contig375 GM03413.5prime 98% Plus / Plus CG14981|FBan0014981|CT34830|FBan0014981 100% Plus / Plus *F bs33f02.y1 AI946971 singleton GH20344.5prime 99% Plus / Plus CG8336|FBan0008336|CT41048|FBan0008336 99% Plus / Plus *F bs33f03.y1 AI946972 singleton none none *F bs33f04.y1 AI946973 singleton LP05771.5prime 99% Plus / Plus CG8788|FBan0008788|CT25336|FBan0008788 99% Plus / Plus *F bs33f05.y1 AI946974 Contig319 GH18530.5prime 99% Plus / Plus CG10143|FBan0010143|CT28535|FBan0010143 99% Plus / Plus *F bs33f06.y1 AI946975 Contig536 GH14152.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs33f07.y1 AI946976 Contig533 GH09435.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs33f08.y1 AI946977 singleton GH10477.5prime 99% Plus / Plus CG7757|FBan0007757|CT23596|FBan0007757 100% Plus / Plus *F bs33f09.y1 AI946978 Contig113 CG8292|FBan0008292|CT24535|FBan0008292 99% Plus / Plus *F bs33f10.y1 AI946979 singleton CG9313|FBan0009313|CT26509|FBan0009313 100% Plus / Plus *F bs33f11.y1 AI946980 Contig403 LP10147.5prime 99% Plus / Plus CG12227|FBan0012227|CT12747|FBan0012227 100% Plus / Plus *F bs33f12.y1 AI946981 singleton CG10541|FBan0010541|CT29571|FBan0010541 99% Plus / Plus *F bs33g01.y1 AI946982 Contig532 GH28719.5prime 98% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 99% Plus / Plus *F bs33g02.y1 AI946983 Contig374 none none *F bs33g03.y1 AI946984 Contig504 CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs33g04.y1 AI946985 singleton LD35227.5prime 99% Plus / Plus CG7014|FBan0007014|CT21704|FBan0007014 98% Plus / Plus *F bs33g05.y1 AI946986 singleton CG5972|FBan0005972|CT18757|FBan0005972 100% Plus / Plus *F bs33g06.y1 AI946987 Contig543 GH11850.5prime 99% Plus / Plus CG4478|FBan0004478|CT14576|FBan0004478 99% Plus / Plus *F bs33g07.y1 AI946988 singleton CG4855|FBan0004855|CT15595|FBan0004855 100% Plus / Plus *F bs33g08.y1 AI946989 Contig511 GH15075.5prime 98% Plus / Plus CG3330|FBan0003330|CT11167|FBan0003330 98% Plus / Plus *F bs33g09.y1 AI946990 singleton CG10407|FBan0010407|CT29228|FBan0010407 98% Plus / Plus *F bs33g10.y1 AI946991 Contig536 CG12860|FBan0012860|CT32000|FBan0012860 100% Plus / Plus *F bs33g11.y1 AI946992 Contig515 HL03427.5prime 99% Plus / Plus none none *F bs33g12.y1 AI946993 Contig111 none none *F bs33h01.y1 AI946994 singleton CG16833|FBan0016833|CT15001|FBan0016833 99% Plus / Plus *F bs33h02.y1 AI946995 Contig328 GH19655.5prime 100% Plus / Plus CG15208|FBan0015208|CT35139|FBan0015208 100% Plus / Plus *F bs33h03.y1 AI946996 singleton GH21448.5prime 95% Plus / Plus CG7046|FBan0007046|CT21791|FBan0007046 100% Plus / Plus *F bs33h04.y1 AI946997 Contig336 CG9632|FBan0009632|CT27234|FBan0009632 99% Plus / Plus *F bs33h05.y1 AI946998 singleton LD05602.3prime 99% Plus / Minus CG8439|FBan0008439|CT24731|FBan0008439 100% Plus / Plus *F bs33h06.y1 AI946999 singleton CG6441|FBan0006441|CT19027|FBan0006441 99% Plus / Plus *F bs33h07.y1 AI947000 Contig530 GH13953.5prime 100% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 100% Plus / Plus *F bs33h08.y1 AI947001 singleton SD01234.5prime 99% Plus / Plus CG6783|FBan0006783|CT21061|FBan0006783 99% Plus / Plus *F bs33h09.y1 pending singleton none none *F bs33h10.y1 AI947002 Contig270 LD24790.5prime 98% Plus / Plus CG10977|FBan0010977|CT30763|FBan0010977 99% Plus / Plus *F bs33h11.y1 AI947003 Contig403 LP10147.5prime 99% Plus / Plus CG12227|FBan0012227|CT12747|FBan0012227 99% Plus / Plus *F bs33h12.y1 pending singleton none none *F bs34a01.y1 AI947004 Contig431 none none *F bs34a02.y1 AI947005 Contig533 GH09435.5prime 99% Plus / Plus CG13245|FBan0013245|CT32496|FBan0013245 98% Plus / Plus *F bs34a03.y1 AI947006 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 97% Plus / Plus *F bs34a04.y1 AI947007 singleton GM02149.5prime 100% Plus / Plus CG6625|FBan0006625|CT20594|FBan0006625 99% Plus / Plus *F bs34a05.y1 AI947008 Contig158 LP12117.5prime 98% Plus / Plus CG7615|FBan0007615|CT8353|FBan0007615 98% Plus / Plus *F bs34a06.y1 AI947009 singleton LD24515.5prime 99% Plus / Plus CG4063|FBan0004063|CT13462|FBan0004063 99% Plus / Plus *F bs34a07.y1 AI947010 Contig540 LP07660.3prime 99% Plus / Minus CG17137|FBan0017137|CT38066|FBan0017137 99% Plus / Plus *F bs34a08.y1 AI947011 singleton LD34803.5prime 97% Plus / Plus CG5300|FBan0005300|CT16873|FBan0005300 97% Plus / Plus *F bs34a09.y1 AI947012 singleton CG18477|FBan0018477|CT42122|FBan0018477 99% Plus / Plus *F bs34a10.y1 AI947013 Contig436 CG12250|FBan0012250|CT14866|FBan0012250 100% Plus / Plus *F bs34a11.y1 AI947014 Contig484 LP08469.5prime 99% Plus / Plus CG5538|FBan0005538|CT17502|FBan0005538 99% Plus / Plus *F bs34a12.y1 AI947015 Contig359 CG12229|FBan0012229|CT12907|FBan0012229 100% Plus / Plus *F bs34b01.y1 AI947016 singleton none none *F bs34b02.y1 AI947017 singleton LP06204.5prime 98% Plus / Plus CG6439|FBan0006439|CT20062|FBan0006439 98% Plus / Plus *F bs34b03.y1 AI947018 Contig543 GH10537.5prime 98% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 98% Plus / Plus *F bs34b04.y1 AI947019 Contig149 LP02662.5prime 99% Plus / Plus CG6822|FBan0006822|CT21141|FBan0006822 99% Plus / Plus *F bs34b05.y1 AI947020 singleton CG10317|FBan0010317|CT28975|FBan0010317 98% Plus / Plus *F bs34b06.y1 AI947021 Contig477 none none *F bs34b07.y1 AI947022 singleton CG6310|FBan0006310|CT19752|FBan0006310 98% Plus / Plus *F bs34b08.y1 AI947023 Contig415 GH05991.5prime 97% Plus / Plus CG9106|FBan0009106|CT26128|FBan0009106 99% Plus / Plus *F bs34b09.y1 AI947024 singleton none none *F bs34b10.y1 AI947025 singleton GH07092.5prime 100% Plus / Plus CG6761|FBan0006761|CT20997|FBan0006761 99% Plus / Plus *F bs34b12.y1 AI947026 singleton none none *F bs34c01.y1 AI947027 Contig282 CG16954|FBan0016954|CT35806|FBan0016954 98% Plus / Plus *F bs34c02.y1 AI947028 Contig504 GH08122.3prime 100% Plus / Minus CG6372|FBan0006372|CT19794|FBan0006372 100% Plus / Plus *F bs34c03.y1 AI947029 Contig544 CG7311|FBan0007311|CT22241|FBan0007311 100% Plus / Plus *F bs34c04.y1 AI947030 singleton GM01061.5prime 99% Plus / Plus CG5000|FBan0005000|CT16014|FBan0005000 100% Plus / Plus *F bs34c05.y1 AI947031 Contig538 GH09790.5prime 99% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 99% Plus / Plus *F bs34c06.y1 pending Contig525 LP01468.5prime 96% Plus / Plus none none *F bs34c07.y1 AI947032 singleton LD41878.5prime 100% Plus / Minus CG10053|FBan0010053|CT28289|FBan0010053 100% Plus / Minus *F bs34c08.y1 AI947033 singleton GH11578.5prime 100% Plus / Plus CG3306|FBan0003306|CT11079|FBan0003306 100% Plus / Plus *F bs34c09.y1 AI947034 Contig236 LD05888.3prime 97% Plus / Minus CG9012|FBan0009012|CT25886|FBan0009012 100% Plus / Plus *F bs34c10.y1 AI947035 singleton LD26005.5prime 82% Plus / Plus CG2241|FBan0002241|CT6882|FBan0002241 100% Plus / Plus *F bs34c11.y1 AI947036 singleton SD10567.3prime 100% Plus / Minus CG3997|FBan0003997|CT13283|FBan0003997 99% Plus / Plus *F bs34c12.y1 AI947037 singleton GH01278.3prime 100% Plus / Minus CG8979|FBan0008979|CT25810|FBan0008979 100% Plus / Plus *F bs34d01.y1 AI947038 Contig198 GH13234.5prime 98% Plus / Plus CG1902|FBan0001902|CT5564|FBan0001902 100% Plus / Plus *F bs34d02.y1 AI947039 Contig541 GH20441.5prime 99% Plus / Plus CG4691|FBan0004691|CT15105|FBan0004691 98% Plus / Plus *F bs34d03.y1 AI947040 singleton CG10104|FBan0010104|CT28441|FBan0010104 100% Plus / Plus *F bs34d04.y1 AI947041 Contig452 GH15825.5prime 99% Plus / Plus CG3085|FBan0003085|CT10370|FBan0003085 99% Plus / Plus *F bs34d05.y1 AI947042 singleton CG5043|FBan0005043|CT16181|FBan0005043 100% Plus / Plus *F bs34d06.y1 AI947043 Contig145 CG7202|FBan0007202|CT22229|FBan0007202 100% Plus / Plus *F bs34d07.y1 AI947044 Contig456 GH23516.5prime 99% Plus / Plus CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs34d08.y1 AI947045 Contig492 CG5443|FBan0005443|CT17278|FBan0005443 99% Plus / Plus *F bs34d09.y1 AI947046 singleton none none *F bs34d10.y1 AI947047 Contig313 GH12317.5prime 99% Plus / Plus CG4994|FBan0004994|CT39178|FBan0004994 99% Plus / Plus *F bs34d12.y1 AI947048 Contig543 GH26012.5prime 98% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 98% Plus / Plus *F bs34e01.y1 AI947049 Contig529 LP09947.5prime 99% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs34e02.y1 AI947050 Contig542 CG13340|FBan0013340|CT32660|FBan0013340 100% Plus / Plus *F bs34e03.y1 AI947051 singleton LP05579.5prime 100% Plus / Plus CG7758|FBan0007758|CT23598|FBan0007758 99% Plus / Plus *F bs34e04.y1 AI947052 singleton GH10766.3prime 98% Plus / Plus CG7148|FBan0007148|CT22091|FBan0007148 99% Plus / Minus *F bs34e05.y1 AI947053 singleton CG6504|FBan0006504|CT20227|FBan0006504 99% Plus / Plus *F bs34e06.y1 pending Contig499 GH19122.3prime 100% Plus / Minus CG18184|FBan0018184|CT41084|FBan0018184 100% Plus / Plus *F bs34e07.y1 AI947054 singleton CG5779|FBan0005779|CT18138|FBan0005779 98% Plus / Plus *F bs34e08.y1 AI947055 Contig379 LD23918.3prime 98% Plus / Minus CG8073|FBan0008073|CT8072|FBan0008073 99% Plus / Plus *F bs34e09.y1 AI947056 Contig214 GH03629.5prime 100% Plus / Plus CG9803|FBan0009803|CT27690|FBan0009803 100% Plus / Plus *F bs34e10.y1 AI947057 Contig199 LP07070.5prime 99% Plus / Minus CG17146|FBan0017146|CT38082|FBan0017146 99% Plus / Plus *F bs34e11.y1 AI947058 Contig530 GH13953.5prime 99% Plus / Plus CG10252|FBan0010252|CT28805|FBan0010252 99% Plus / Plus *F bs34e12.y1 AI947059 singleton LD21694.3prime 98% Plus / Minus CG2980|FBan0002980|CT40308|FBan0002980 99% Plus / Plus *F bs34f01.y1 AI947060 singleton GH25749.5prime 100% Plus / Plus CG5474|FBan0005474|CT17318|FBan0005474 99% Plus / Plus *F bs34f02.y1 AI947061 Contig493 CG14926|FBan0014926|CT34753|FBan0014926 98% Plus / Plus *F bs34f03.y1 AI947062 Contig494 CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs34f04.y1 AI947063 Contig483 CG15200|FBan0015200|CT35127|FBan0015200 100% Plus / Plus *F bs34f05.y1 AI947064 Contig538 GH09790.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs34f06.y1 AI947065 Contig317 none none *F bs34f07.y1 AI947066 Contig544 none none *F bs34f08.y1 AI947067 Contig096 CG5662|FBan0005662|CT17888|FBan0005662 99% Plus / Plus *F bs34f09.y1 AI947068 singleton LD14503.5prime 100% Plus / Plus CG15812|FBan0015812|CT31734|FBan0015812 99% Plus / Plus *F bs34f10.y1 AI947069 singleton LD15362.5prime 96% Plus / Plus CG8316|FBan0008316|CT24555|FBan0008316 98% Plus / Plus *F bs34f11.y1 AI947070 Contig487 CG12470|FBan0012470|CT32706|FBan0012470 100% Plus / Plus *F bs34f12.y1 AI947071 Contig232 GH15908.5prime 99% Plus / Plus CG4686|FBan0004686|CT15101|FBan0004686 99% Plus / Plus *F bs34g01.y1 AI947072 Contig285 LP03919.5prime 99% Plus / Plus CG7701|FBan0007701|CT23463|FBan0007701 99% Plus / Plus *F bs34g02.y1 AI947073 Contig503 GH11521.5prime 96% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 96% Plus / Plus *F bs34g03.y1 AI947074 Contig413 CG7750|FBan0007750|CT23586|FBan0007750 100% Plus / Plus *F bs34g04.y1 pending singleton CG14546|FBan0014546|CT34276|FBan0014546 99% Plus / Plus *F bs34g06.y1 AI947075 Contig394 CG7059|FBan0007059|CT21831|FBan0007059 99% Plus / Plus *F bs34g07.y1 AI947076 Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs34g08.y1 AI947077 Contig239 GH09348.5prime 98% Plus / Plus CG12790|FBan0012790|CT37283|FBan0012790 98% Plus / Plus *F bs34g09.y1 AI947078 singleton CG2921|FBan0002921|CT9015|FBan0002921 99% Plus / Plus *F bs34g10.y1 AI947079 Contig513 CG11719|FBan0011719|CT5056|FBan0011719 100% Plus / Plus *F bs34g11.y1 AI947080 Contig225 GM04907.5prime 97% Plus / Plus CG1469|FBan0001469|CT3604|FBan0001469 99% Plus / Plus *F bs34g12.y1 AI947081 Contig453 CG9314|FBan0009314|CT26521|FBan0009314 99% Plus / Plus *F bs34h01.y1 AI947082 Contig404 CG14718|FBan0014718|CT34509|FBan0014718 99% Plus / Plus *F bs34h02.y1 AI947083 singleton LP06958.5prime 98% Plus / Plus CG4918|FBan0004918|CT15790|FBan0004918 98% Plus / Plus *F bs34h03.y1 AI947084 Contig333 LD33142.3prime 99% Plus / Minus CG2512|FBan0002512|CT8333|FBan0002512 100% Plus / Plus *F bs34h04.y1 AI947085 Contig151 GH01518.5prime 99% Plus / Plus CG2727|FBan0002727|CT9269|FBan0002727 99% Plus / Plus *F bs34h05.y1 AI947086 singleton none none *F bs34h06.y1 AI947087 Contig219 CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs34h07.y1 AI947088 Contig356 none none *F bs34h08.y1 AI947089 singleton LD11842.5prime 96% Plus / Plus CG9705|FBan0009705|CT27440|FBan0009705 99% Plus / Plus *F bs34h09.y1 AI947090 Contig529 LP04643.5prime 99% Plus / Plus CG6372|FBan0006372|CT19736|FBan0006372 99% Plus / Plus *F bs34h10.y1 AI947091 singleton LD40681.5prime 99% Plus / Plus CG10130|FBan0010130|CT28511|FBan0010130 99% Plus / Plus *F bs34h11.y1 AI947092 Contig160 none none *F bs34h12.y1 AI947093 singleton GH13113.5prime 100% Plus / Plus CG7958|FBan0007958|CT41698|FBan0007958 100% Plus / Plus *F bs35a01.y1 AI947094 Contig544 GH22513.5prime 98% Plus / Plus none none *F bs35a02.y1 AI947095 Contig159 GH27147.5prime 99% Plus / Plus CG3199|FBan0003199|CT10703|FBan0003199 99% Plus / Plus *F bs35a03.y1 AI947096 Contig503 GH11521.5prime 100% Plus / Plus CG12907|FBan0012907|CT32052|FBan0012907 100% Plus / Plus *F bs35a04.y1 pending Contig510 GH21762.5prime 92% Plus / Plus CG4959|FBan0004959|CT15918|FBan0004959 92% Plus / Plus *F bs35a05.y1 AI947097 Contig222 GH27752.5prime 99% Plus / Plus CG8813|FBan0008813|CT10113|FBan0008813 99% Plus / Plus *F bs35a06.y1 AI947098 Contig518 GH22658.5prime 96% Plus / Plus CG18461|FBan0018461|CT33258|FBan0018461 96% Plus / Plus *F bs35a07.y1 AI947099 singleton none none *F bs35a09.y1 pending Contig531 CG4836|FBan0004836|CT15517|FBan0004836 97% Plus / Plus *F bs35a11.y1 pending Contig422 GH23808.5prime 100% Plus / Plus CG9133|FBan0009133|CT10073|FBan0009133 99% Plus / Minus *F bs35b01.y1 AI947100 Contig344 CG7094|FBan0007094|CT21551|FBan0007094 100% Plus / Plus *F bs35b03.y1 AI947101 singleton LD12690.5prime 98% Plus / Plus CG9829|FBan0009829|CT27760|FBan0009829 98% Plus / Plus *F bs35b04.y1 AI947102 singleton CG9064|FBan0009064|CT26034|FBan0009064 99% Plus / Plus *F bs35b05.y1 AI947103 singleton HL03528.5prime 99% Plus / Plus CG1640|FBan0001640|CT4458|FBan0001640 100% Plus / Plus *F bs35b06.y1 AI947104 singleton CG17717|FBan0017717|CT39281|FBan0017717 99% Plus / Plus *F bs35b07.y1 AI947105 singleton GH02503.5prime 100% Plus / Plus CG2140|FBan0002140|CT6982|FBan0002140 100% Plus / Plus *F bs35b08.y1 AI947106 singleton CG5653|FBan0005653|CT17866|FBan0005653 100% Plus / Plus *F bs35b09.y1 AI947107 singleton CG7637|FBan0007637|CT23315|FBan0007637 99% Plus / Plus *F bs35b10.y1 pending Contig086 none none *F bs35b11.y1 AI947108 Contig240 CG15260|FBan0015260|CT35204|FBan0015260 99% Plus / Plus *F bs35b12.y1 AI947109 Contig171 CG12983|FBan0012983|CT32175|FBan0012983 100% Plus / Minus *F bs35c01.y1 pending Contig532 CG17349|FBan0017349|CT32310|FBan0017349 99% Plus / Plus *F bs35c02.y1 AI947110 singleton LP06734.5prime 100% Plus / Plus CG3699|FBan0003699|CT12411|FBan0003699 99% Plus / Plus *F bs35c03.y1 AI947111 Contig406 GH05530.5prime 99% Plus / Plus CG17567|FBan0017567|CT35086|FBan0017567 99% Plus / Plus *F bs35c04.y1 AI947112 Contig298 CG15711|FBan0015711|CT35936|FBan0015711 98% Plus / Plus *F bs35c05.y1 AI947113 Contig507 LP05356.5prime 99% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs35c06.y1 AI947114 Contig449 CG9218|FBan0009218|CT26312|FBan0009218 100% Plus / Plus *F bs35c07.y1 AI947115 Contig531 GH04958.5prime 100% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs35c08.y1 AI947116 Contig471 none none *F bs35c09.y1 AI947117 Contig278 LP11475.5prime 99% Plus / Plus CG15109|FBan0015109|CT42559|FBan0015109 99% Plus / Plus *F bs35c10.y1 AI947118 singleton GH21824.5prime 99% Plus / Plus CG6833|FBan0006833|CT21153|FBan0006833 99% Plus / Plus *F bs35c11.y1 AI947119 Contig306 GM04388.5prime 97% Plus / Plus CG10191|FBan0010191|CT28607|FBan0010191 98% Plus / Plus *F bs35d01.y1 AI947120 singleton GH28095.5prime 99% Plus / Plus none none *F bs35d02.y1 AI947121 singleton GH18521.5prime 98% Plus / Plus CG8838|FBan0008838|CT11075|FBan0008838 99% Plus / Plus *F bs35d03.y1 AI947122 singleton CG15608|FBan0015608|CT35744|FBan0015608 100% Plus / Plus *F bs35d04.y1 AI947123 Contig136 CG15605|FBan0015605|CT35737|FBan0015605 99% Plus / Plus *F bs35d05.y1 AI947124 Contig176 HL02448.5prime 100% Plus / Plus CG6084|FBan0006084|CT19142|FBan0006084 100% Plus / Plus *F bs35d06.y1 AI947125 Contig431 none none *F bs35d07.y1 AI947126 singleton GH19966.5prime 99% Plus / Plus CG9263|FBan0009263|CT26206|FBan0009263 99% Plus / Plus *F bs35d08.y1 AI947127 Contig535 GH14562.5prime 99% Plus / Plus CG3315|FBan0003315|CT11141|FBan0003315 99% Plus / Plus *F bs35d09.y1 AI947128 singleton CG7975|FBan0007975|CT24000|FBan0007975 98% Plus / Plus *F bs35d10.y1 AI947129 singleton CG9214|FBan0009214|CT26324|FBan0009214 99% Plus / Plus *F bs35d11.y1 AI947130 singleton CG5213|FBan0005213|CT16671|FBan0005213 100% Plus / Plus *F bs35d12.y1 AI947131 Contig531 GH04958.5prime 100% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs35e01.y1 AI947132 Contig420 SD02991.5prime 100% Plus / Minus CG6105|FBan0006105|CT19171|FBan0006105 100% Plus / Plus *F bs35e02.y1 AI947133 Contig483 CG15200|FBan0015200|CT35127|FBan0015200 100% Plus / Plus *F bs35e03.y1 AI947134 singleton CG16974|FBan0016974|CT35580|FBan0016974 100% Plus / Plus *F bs35e04.y1 AI947135 singleton CG12400|FBan0012400|CT27284|FBan0012400 100% Plus / Plus *F bs35e05.y1 AI947136 Contig315 GH14739.5prime 100% Plus / Plus CG7425|FBan0007425|CT22777|FBan0007425 100% Plus / Plus *F bs35e06.y1 AI947137 singleton LD19244.5prime 99% Plus / Minus CG8677|FBan0008677|CT5294|FBan0008677 99% Plus / Minus *F bs35e07.y1 AI947138 Contig108 none none *F bs35e08.y1 AI947139 singleton CG8494|FBan0008494|CT24839|FBan0008494 99% Plus / Plus *F bs35e09.y1 AI947140 singleton CG18234|FBan0018234|CT41275|FBan0018234 99% Plus / Plus *F bs35e10.y1 AI947141 singleton CG3748|FBan0003748|CT12531|FBan0003748 99% Plus / Plus *F bs35e11.y1 AI947142 singleton GH19618.5prime 99% Plus / Plus CG6871|FBan0006871|CT21282|FBan0006871 99% Plus / Plus *F bs35e12.y1 AI947143 singleton GH20124.5prime 100% Plus / Plus CG8489|FBan0008489|CT24829|FBan0008489 100% Plus / Plus *F bs35f01.y1 AI947144 Contig092 none none *F bs35f02.y1 AI947145 Contig113 CG8292|FBan0008292|CT24535|FBan0008292 98% Plus / Plus *F bs35f03.y1 AI947146 singleton GM06533.5prime 97% Plus / Plus CG7768|FBan0007768|CT39154|FBan0007768 98% Plus / Plus *F bs35f05.y1 AI947147 singleton GH16566.3prime 99% Plus / Minus CG5753|FBan0005753|CT39078|FBan0005753 99% Plus / Plus *F bs35f06.y1 AI947148 Contig222 GH27752.5prime 99% Plus / Plus CG8813|FBan0008813|CT10113|FBan0008813 99% Plus / Plus *F bs35f07.y1 AI947149 Contig357 SD02767.5prime 100% Plus / Plus CG13176|FBan0013176|CT32417|FBan0013176 100% Plus / Plus *F bs35f08.y1 AI947150 singleton CG8851|FBan0008851|CT9281|FBan0008851 99% Plus / Plus *F bs35f09.y1 AI947151 Contig515 HL02093.5prime 100% Plus / Plus none none *F bs35f10.y1 AI947152 Contig466 GH20874.5prime 99% Plus / Plus CG2267|FBan0002267|CT7048|FBan0002267 99% Plus / Plus *F bs35f11.y1 AI947153 Contig472 GH19274.5prime 88% Plus / Minus CG9129|FBan0009129|CT8135|FBan0009129 99% Plus / Minus *F bs35f12.y1 AI947154 Contig544 GH23081.5prime 99% Plus / Plus CG17956|FBan0017956|CT40004|FBan0017956 100% Plus / Plus *F bs35g01.y1 AI947155 Contig540 none none *F bs35g02.y1 AI947156 Contig526 GH11587.5prime 98% Plus / Plus CG1324|FBan0001324|CT2936|FBan0001324 99% Plus / Plus *F bs35g03.y1 AI947157 Contig516 GH01662.3prime 98% Plus / Minus CG3982|FBan0003982|CT13237|FBan0003982 99% Plus / Plus *F bs35g04.y1 AI947158 Contig454 CG2164|FBan0002164|CT7072|FBan0002164 99% Plus / Plus *F bs35g05.y1 AI947159 Contig538 GH09790.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs35g06.y1 AI947160 Contig544 CG5790|FBan0005790|CT18162|FBan0005790 97% Plus / Plus *F bs35g07.y1 AI947161 Contig540 none none *F bs35g08.y1 AI947162 singleton none none *F bs35g09.y1 AI947163 Contig125 GH13025.3prime 99% Plus / Minus CG16901|FBan0016901|CT34004|FBan0016901 99% Plus / Plus *F bs35g10.y1 AI947164 Contig494 GH10403.5prime 99% Plus / Plus CG3213|FBan0003213|CT10803|FBan0003213 99% Plus / Plus *F bs35g11.y1 AI947165 Contig463 CG10690|FBan0010690|CT29964|FBan0010690 100% Plus / Minus *F bs35g12.y1 AI947166 singleton LP04056.5prime 99% Plus / Plus CG12363|FBan0012363|CT24202|FBan0012363 99% Plus / Plus *F bs35h01.y1 AI947167 singleton LD27106.5prime 99% Plus / Plus CG10372|FBan0010372|CT29128|FBan0010372 99% Plus / Plus *F bs35h02.y1 AI947168 Contig184 CG8244|FBan0008244|CT23727|FBan0008244 100% Plus / Plus *F bs35h03.y1 AI947169 singleton none none *F bs35h04.y1 AI947170 singleton LD22982.5prime 99% Plus / Plus CG1228|FBan0001228|CT2090|FBan0001228 100% Plus / Plus *F bs35h05.y1 AI947171 Contig216 CG2337|FBan0002337|CT7790|FBan0002337 98% Plus / Plus *F bs35h07.y1 AI947172 Contig175 none none *F bs35h08.y1 AI947173 Contig544 GH14656.5prime 99% Plus / Plus CG12699|FBan0012699|CT35734|FBan0012699 99% Plus / Plus *F bs35h09.y1 AI947174 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 100% Plus / Plus *F bs35h10.y1 AI947175 Contig456 GH23516.5prime 99% Plus / Plus CG2149|FBan0002149|CT7028|FBan0002149 99% Plus / Plus *F bs35h12.y1 AI947176 Contig410 CG17946|FBan0017946|CT39984|FBan0017946 100% Plus / Plus *F bs36a01.y1 pending singleton GH02666.5prime 99% Plus / Plus CG10565|FBan0010565|CT29638|FBan0010565 99% Plus / Plus *F bs36a02.y1 AI947177 Contig536 GH14152.5prime 100% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 99% Plus / Plus *F bs36a03.y1 pending Contig534 none none *F bs36a04.y1 AI947178 Contig411 CG11125|FBan0011125|CT8285|FBan0011125 100% Plus / Plus *F bs36a05.y1 AI947179 Contig345 LP02468.5prime 99% Plus / Plus CG9412|FBan0009412|CT37417|FBan0009412 100% Plus / Plus *F bs36a06.y1 AI947180 Contig457 GH18334.5prime 99% Plus / Plus CG6255|FBan0006255|CT19574|FBan0006255 100% Plus / Plus *F bs36a07.y1 AI947181 Contig493 CG14926|FBan0014926|CT34753|FBan0014926 98% Plus / Plus *F bs36a08.y1 AI947182 Contig491 GH26552.5prime 99% Plus / Plus CG8136|FBan0008136|CT24268|FBan0008136 99% Plus / Plus *F bs36a09.y1 AI947183 singleton GH06537.5prime 100% Plus / Plus CG1903|FBan0001903|CT5846|FBan0001903 99% Plus / Plus *F bs36a10.y1 AI947184 Contig315 GH19904.5prime 99% Plus / Plus CG7425|FBan0007425|CT22777|FBan0007425 99% Plus / Plus *F bs36a11.y1 AI947185 Contig514 GH04665.5prime 95% Plus / Plus none none *F bs36a12.y1 pending Contig111 none none *F bs36b01.y1 AI947186 singleton LP02089.5prime 98% Plus / Plus CG13385|FBan0013385|CT32731|FBan0013385 98% Plus / Plus *F bs36b02.y1 AI947187 singleton GH05530.5prime 99% Plus / Plus CG17567|FBan0017567|CT35086|FBan0017567 99% Plus / Plus *F bs36b03.y1 AI947188 Contig461 LP08687.5prime 96% Plus / Plus CG6372|FBan0006372|CT19794|FBan0006372 96% Plus / Plus *F bs36b04.y1 AI947189 singleton LP09540.5prime 99% Plus / Plus CG16983|FBan0016983|CT40292|FBan0016983 99% Plus / Plus *F bs36b05.y1 AI947190 singleton CG15662|FBan0015662|CT35846|FBan0015662 99% Plus / Plus *F bs36b06.y1 AI947191 singleton CG13222|FBan0013222|CT32466|FBan0013222 100% Plus / Plus *F bs36b07.y1 AI947192 Contig257 GH08902.3prime 93% Plus / Minus CG12562|FBan0012562|CT34305|FBan0012562 99% Plus / Plus *F bs36b08.y1 AI947193 Contig501 CG9975|FBan0009975|CT28093|FBan0009975 98% Plus / Plus *F bs36b09.y1 AI947194 singleton GH17405.5prime 99% Plus / Plus CG12207|FBan0012207|CT11006|FBan0012207 98% Plus / Plus *F bs36b10.y1 AI947195 Contig489 GH10833.3prime 98% Plus / Minus CG5045|FBan0005045|CT16189|FBan0005045 98% Plus / Plus *F bs36b11.y1 AI947196 Contig508 GH01608.3prime 100% Plus / Minus CG8994|FBan0008994|CT41874|FBan0008994 100% Plus / Plus *F bs36b12.y1 AI947197 singleton CG1315|FBan0001315|CT2879|FBan0001315 99% Plus / Minus *F bs36c01.y1 pending Contig533 GH09435.5prime 91% Plus / Plus none none *F bs36c02.y1 AI947198 Contig543 GH26012.5prime 100% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 100% Plus / Plus *F bs36c03.y1 AI947199 singleton CG13167|FBan0013167|CT32408|FBan0013167 98% Plus / Plus *F bs36c04.y1 AI947200 singleton none none *F bs36c05.y1 AI947201 Contig158 LP12117.5prime 98% Plus / Plus CG7615|FBan0007615|CT8353|FBan0007615 98% Plus / Plus *F bs36c06.y1 AI947202 Contig434 CG7164|FBan0007164|CT22119|FBan0007164 100% Plus / Plus *F bs36c07.y1 AI947203 Contig481 CG5051|FBan0005051|CT16124|FBan0005051 100% Plus / Plus *F bs36c08.y1 AI947204 Contig538 GH10186.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs36c09.y1 AI947205 Contig415 GH05991.5prime 98% Plus / Plus CG9106|FBan0009106|CT26128|FBan0009106 98% Plus / Plus *F bs36c10.y1 AI947206 singleton GH05770.5prime 99% Plus / Plus CG5364|FBan0005364|CT17046|FBan0005364 99% Plus / Plus *F bs36c11.y1 AI947207 Contig521 GH15271.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs36c12.y1 AI947208 Contig484 LP08469.5prime 99% Plus / Plus CG5538|FBan0005538|CT17502|FBan0005538 99% Plus / Plus *F bs36d01.y1 AI947209 Contig515 HL02977.5prime 99% Plus / Plus none none *F bs36d02.y1 AI947210 singleton CG5139|FBan0005139|CT16455|FBan0005139 99% Plus / Plus *F bs36d03.y1 AI947211 singleton CG9777|FBan0009777|CT27615|FBan0009777 100% Plus / Plus *F bs36d04.y1 AI947212 Contig536 GH19031.5prime 98% Plus / Plus CG12860|FBan0012860|CT32000|FBan0012860 98% Plus / Plus *F bs36d05.y1 AI947213 Contig465 CG4434|FBan0004434|CT14426|FBan0004434 99% Plus / Plus *F bs36d06.y1 AI947214 singleton GH21622.5prime 100% Plus / Plus CG6652|FBan0006652|CT20666|FBan0006652 99% Plus / Plus *F bs36d07.y1 AI947215 Contig324 CG8476|FBan0008476|CT24775|FBan0008476 97% Plus / Plus *F bs36d08.y1 AI947216 Contig436 CG12250|FBan0012250|CT14866|FBan0012250 100% Plus / Plus *F bs36d09.y1 AI947217 Contig500 LD35818.5prime 99% Plus / Plus CG6513|FBan0006513|CT20269|FBan0006513 99% Plus / Plus *F bs36d10.y1 AI947218 Contig235 GH28564.5prime 84% Plus / Plus CG7235|FBan0007235|CT22309|FBan0007235 100% Plus / Plus *F bs36d11.y1 AI947219 Contig507 LP03126.5prime 99% Plus / Plus CG4799|FBan0004799|CT15419|FBan0004799 99% Plus / Plus *F bs36d12.y1 pending Contig162 LD13184.5prime 98% Plus / Minus CG6661|FBan0006661|CT20682|FBan0006661 98% Plus / Plus *F bs36e02.y1 AI947220 singleton LD26575.5prime 99% Plus / Plus CG6022|FBan0006022|CT18900|FBan0006022 99% Plus / Plus *F bs36e03.y1 AI947221 singleton GH07590.5prime 100% Plus / Plus CG6218|FBan0006218|CT19478|FBan0006218 100% Plus / Plus *F bs36e04.y1 pending Contig293 LP12359.5prime 95% Plus / Plus CG2277|FBan0002277|CT7561|FBan0002277 95% Plus / Plus *F bs36e05.y1 AI947222 Contig179 LD29127.5prime 100% Plus / Minus CG7414|FBan0007414|CT22817|FBan0007414 100% Plus / Plus *F bs36e06.y1 AI947223 singleton GH18913.5prime 98% Plus / Plus CG10841|FBan0010841|CT30359|FBan0010841 99% Plus / Plus *F bs36e07.y1 AI947224 Contig310 CG9406|FBan0009406|CT26694|FBan0009406 98% Plus / Plus *F bs36e08.y1 AI947225 Contig456 CG2149|FBan0002149|CT7028|FBan0002149 100% Plus / Plus *F bs36e09.y1 AI947226 Contig522 LP09246.5prime 98% Plus / Plus CG13414|FBan0013414|CT32770|FBan0013414 91% Plus / Plus *F bs36e10.y1 AI947227 Contig531 GH04958.5prime 100% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs36e11.y1 AI947228 singleton CG7295|FBan0007295|CT22523|FBan0007295 98% Plus / Plus *F bs36e12.y1 AI947229 Contig352 GH17362.5prime 99% Plus / Plus CG12313|FBan0012313|CT21286|FBan0012313 99% Plus / Plus *F bs36f01.y1 pending Contig543 GH12543.5prime 93% Plus / Plus CG4750|FBan0004750|CT15217|FBan0004750 93% Plus / Plus *F bs36f02.y1 AI947230 singleton CG3788|FBan0003788|CT12610|FBan0003788 100% Plus / Plus *F bs36f03.y1 AI947231 Contig521 GH27033.5prime 99% Plus / Plus CG2127|FBan0002127|CT6944|FBan0002127 99% Plus / Plus *F bs36f04.y1 AI947232 Contig517 CG8517|FBan0008517|CT24849|FBan0008517 99% Plus / Plus *F bs36f05.y1 AI947233 Contig532 LP11026.5prime 100% Plus / Plus CG8040|FBan0008040|CT24130|FBan0008040 100% Plus / Plus *F bs36f06.y1 AI947234 Contig544 CG12902|FBan0012902|CT32047|FBan0012902 99% Plus / Plus *F bs36f07.y1 AI947235 Contig534 none none *F bs36f08.y1 AI947236 Contig531 GH04958.5prime 100% Plus / Plus CG4836|FBan0004836|CT15517|FBan0004836 99% Plus / Plus *F bs36f09.y1 AI947237 Contig540 GH25305.5prime 100% Plus / Plus CG9602|FBan0009602|CT27154|FBan0009602 100% Plus / Plus *F bs36f10.y1 AI947238 Contig138 GH01042.5prime 98% Plus / Plus CG17401|FBan0017401|CT38419|FBan0017401 100% Plus / Plus *F bs36f11.y1 AI947239 Contig423 GM02127.5prime 100% Plus / Plus CG1383|FBan0001383|CT3168|FBan0001383 100% Plus / Plus *F bs36f12.y1 AI947240 Contig509 GM13259.5prime 98% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 98% Plus / Plus *F bs36g01.y1 pending Contig219 CG2149|FBan0002149|CT7028|FBan0002149 94% Plus / Plus *F bs36g02.y1 AI947241 Contig543 CG4750|FBan0004750|CT15217|FBan0004750 98% Plus / Plus *F bs36g03.y1 AI947242 Contig259 CG9329|FBan0009329|CT3799|FBan0009329 99% Plus / Plus *F bs36g04.y1 AI947243 Contig416 LD17341.5prime 99% Plus / Minus CG6319|FBan0006319|CT19690|FBan0006319 100% Plus / Plus *F bs36g05.y1 AI947244 Contig525 LP11945.5prime 97% Plus / Plus CG11064|FBan0011064|CT30951|FBan0011064 97% Plus / Minus *F bs36g06.y1 AI947245 singleton CG14644|FBan0014644|CT34416|FBan0014644 100% Plus / Plus *F bs36g07.y1 AI947246 Contig451 GH08251.5prime 99% Plus / Plus CG18628|FBan0018628|CT41623|FBan0018628 99% Plus / Plus *F bs36g08.y1 AI947247 singleton LD47727.5prime 99% Plus / Plus CG8786|FBan0008786|CT25334|FBan0008786 99% Plus / Plus *F bs36g09.y1 AI947248 singleton none none *F bs36g10.y1 AI947249 Contig210 none none *F bs36g11.y1 AI947250 Contig540 none none *F bs36g12.y1 AI947251 Contig544 LP06279.5prime 100% Plus / Plus CG17377|FBan0017377|CT38384|FBan0017377 99% Plus / Plus *F bs36h01.y1 AI947252 Contig112 LP11793.3prime 97% Plus / Minus CG2164|FBan0002164|CT7072|FBan0002164 98% Plus / Plus *F bs36h02.y1 AI947253 Contig382 CG8750|FBan0008750|CT25256|FBan0008750 99% Plus / Plus *F bs36h03.y1 AI947254 singleton CG3544|FBan0003544|CT11910|FBan0003544 99% Plus / Plus *F bs36h04.y1 AI947255 Contig472 GH22559.5prime 82% Plus / Plus CG9129|FBan0009129|CT8135|FBan0009129 99% Plus / Plus *F bs36h05.y1 AI947256 Contig509 GM13259.5prime 99% Plus / Plus CG1683|FBan0001683|CT4708|FBan0001683 99% Plus / Plus *F bs36h06.y1 AI947257 singleton LD09945.5prime 99% Plus / Plus CG3587|FBan0003587|CT12059|FBan0003587 100% Plus / Plus *F bs36h07.y1 AI947258 singleton LP07544.5prime 99% Plus / Plus CG6259|FBan0006259|CT19586|FBan0006259 99% Plus / Plus *F bs36h08.y1 AI947259 Contig538 GH09790.5prime 98% Plus / Plus CG12861|FBan0012861|CT32001|FBan0012861 98% Plus / Plus *F bs36h09.y1 AI947260 Contig489 GH10833.5prime 99% Plus / Plus CG5045|FBan0005045|CT16189|FBan0005045 100% Plus / Plus *F bs36h10.y1 AI947261 Contig312 GH02649.5prime 100% Plus / Plus CG7366|FBan0007366|CT22689|FBan0007366 100% Plus / Plus *F bs36h11.y1 AI947262 singleton GH11778.5prime 99% Plus / Minus CG6871|FBan0006871|CT21282|FBan0006871 99% Plus / Plus *F bs36h12.y1 AI947263 singleton GM13131.3prime 100% Plus / Minus CG11143|FBan0011143|CT31151|FBan0011143 100% Plus / Plus # *U FBrf0137493 *a Millburn *b G. *t 2001.8.17 *T personal communication to FlyBase *u FlyBase error report for CG12884 on Fri Aug 17 04:16:22 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 17 Aug 2001 04:16:22 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase error report for CG12884 on Fri Aug 17 04:16:22 2001 *F Error report from Gillian Millburn (gm119@gen.cam.ac.uk) *F Gene or accession: CG12884 *F Release: 1 *F Gene annotation error *F Gene CG12884 should be split. *F Comments: hello, *F 3 gustatory receptor (GR) genes match the genome in the region of the *F CG12884 annotation, indicating that CG12884 may need splitting, or the *F three GR genes may need merging. *F The three GR genes are: *F from http://cpmcnet.columbia.edu/dept/neurobeh/axel/gr.html (being *F curated as Scott and Axel, 2001.4.25, personal communication to *F FlyBase): 'Gr98A1' and 'Gr98A2'. *F >From Clyne et al., 2000, Science 287(5459): 1830--1834 (FBrf0126794): *F 'GR98B.1'. *F In addition, 'Gr98A1' and 'GR98B.1' overlap somewhat so may need *F merging. *F Sequences of the 3 GR genes and alignments follow in e-mail *F to flybase-updates, *F Gillian *F From gm119@gen.cam.ac.uk Fri Aug 17 12:17:52 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 17 Aug 2001 12:17:52 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: re: error report for CG12884 *F X-Sun-Charset: US-ASCII *F From: Gillian Millburn (Genetics) *F Date: Fri, 17 Aug 2001 12:17:48 \+0100 *F Content-Length: 29946 *F >Gr98A1 *F MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFIVSYFNIIAIDEEVLEYNVS *F DFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIADLEMDMDEASQCFGGQRQRFSFRFRMALCVGVWMILMVG *F SMPRLTMTAMGPFVSTLLKILTEFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKR *F NQLLLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYGPEYCLFVLLVYELILRTRHVLEQL *F KDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLMSF *F HFSLNMEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIYECYANIVALQKDIH *F KFHAEDSSKVMGNTQKVLVVAMFVWNQLNILLNFRRLARIYDDIADLEIDLNNASSGFVGQRHWWRFRFRLALSVGLWI *F VLLVGLTPRFTLVALGPYLHWTNKVLTEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELC *F VALKRNQLLAGRIWGLVNEVSLYFTLSLTLLFLYNELTILQIVNWALIKSVNPNECCQYTEDYLILKMGLREYSLQMEH *F LKLIFTCGGLFDINLKFFGGVKLKL *F >Gr98A2 *F MEAKRSRLLTTARPYLQVLSLFGLTPPAEFFTRTLRKRRRFCWMAGYSLYLIAILLMVFYEFHANIVSLHLEIYKFHVE *F DFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIANLDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVIIIG *F VIPRLTLGRAGPFFHWVNQVLTQIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQ *F NQLLIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE *F >GR98B.1,AC007817,45506-46916 *F atggaagccaatcggagtcgtctgctggccgcagcgcgtccttacattcagatttattccattttcggactcacgccgc *F caattcagttttttaccaggaccttacataagcgacgtagaggaattgtgatattgggctacgcctgctatttaattag *F catttccctgatggtcatctatgagtgctacgcgaacattgtggctctgcaaaaggatatacataagtttcacgccgag *F gactctagcaaagttatgggggaatacgcagaaaggtcctggtgggtagccatgttcgtttggaatcaattgaacattc *F tgcttaactttcggcgccttgctaggatttatgatgatattgcggatctggaaatagatttgaataacgcctctagcgg *F ttttgttggccaacggcactggtggcgcttccgtttccggttggccctctctgtgggcctgtggatagtgttgctggtg *F ggtctcacgccacgattcaccctcgtggcactcggaccctacctccactggacaaataaagtgctcaccgaaatcattc *F tgataatgctacaacttaagtgtacagagtattgtgtgtttgtgctcctgatctatgaactgatcctccgagggcgcca *F catccttcagcagatcagtgtggagctcgagggtaaccagtcaagggacagtgttcaggagctgtgtgtggccttgaaa *F cgcaatcagttgctggctggacgcatttggggcttggtgaatgaggtcagcttgtattttaccctatccttgacgcttt *F tgtttctctacaatgaactgaccattctgcaaattgtcaattgggctctcattaaatccgtcaatccaaacgaatgctg *F tcaatatagtaagttagttttcaagttcaaaagaaactttacctataaacaagttattttcataatagggcgcgttggt *F acttgcctcttgctgtcaatcaatatttttctatcctgtttat! *F acagcgagttctgcattcaaacagtaagtatacctctataattctttaaaatgtttttaataattttatttttataagt *F ataatagcatttcacgagttcttcaccaaatgtattgcctttctgcagccgaagattatctaatattaaaaatgggcct *F gagggaatactcgctgcaaatggagcatttaaagctgattttcacatgcggtggcctctttgacatcaatcttaagttc *F ttcggaggggtaaaacttaaattataaattaaaaatcaagcttatgtacactttcctttcttctagatggtagtcacct *F tattcggttatatcattattctcgtgcaatttaaaattcaattttttgctcaatcaaattttatgcaaaatattaacag *F caccgaactgaaagcatataccgcg *F Query= Gr98A1 , 736 bases, BF190611 checksum. *F (736 letters) *F Database: /data/blast/db/aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG12884|FBan0012884|CT32028|FBan0012884 last_updated:000321 3508 0.0 1 *F CG13788|FBan0013788|CT33277|FBan0013788 last_updated:000321 72 0.093 2 *F CG18531|FBan0018531|CT42308|FBan0018531 last_updated:000321 74 0.28 2 *F CG17207|FBan0017207|CT34543|FBan0017207 last_updated:000321 67 0.994 1 *F CG14979|FBan0014979|CT34827|FBan0014979 last_updated:000321 65 0.997 2 *F CG13602|FBan0013602|CT32987|FBan0013602 last_updated:000321 64 0.999 1 *F CG10922|FBan0010922|CT30569|FBan0010922 last_updated:000321 68 0.99990 1 *F >CG12884|FBan0012884|CT32028|FBan0012884 last_updated:000321 *F Length = 736 *F Score = 3508 (1239.9 bits), Expect = 0.0, P = 0.0 *F Identities = 685/736 (93%), Positives = 685/736 (93%) *F Query: 1 MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI 60 *F MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI *F Sbjct: 1 MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI 60 *F Query: 61 VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIAX 120 *F VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIA *F Sbjct: 61 VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIAD 120 *F Query: 121 XXXXXXEASQCXXXXXXXXXXXXXMALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT 180 *F EASQC MALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT *F Sbjct: 121 LEMDMDEASQCFGGQRQRFSFRFRMALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT 180 *F Query: 181 EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL 240 *F EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL *F Sbjct: 181 EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL 240 *F Query: 241 LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYGPEYCLFVL 300 *F LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYGPEYCLFVL *F Sbjct: 241 LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYGPEYCLFVL 300 *F Query: 301 LVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVDEIGAYFRWS 360 *F LVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVDEIGAYFRWS *F Sbjct: 301 LVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVDEIGAYFRWS 360 *F Query: 361 MTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLMSFHFSLNMEANRSRLLAAARPYIQIYS 420 *F MTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLMSFHFSLNMEANRSRLLAAARPYIQIYS *F Sbjct: 361 MTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLMSFHFSLNMEANRSRLLAAARPYIQIYS 420 *F Query: 421 IFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIYECYANIVALQKDIHKFHAED 480 *F IFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIYECYANIVALQKDIHKFHAED *F Sbjct: 421 IFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIYECYANIVALQKDIHKFHAED 480 *F Query: 481 SSKVMGNTQKVLVVAMFVWNQLNILLNFRRLARIYDDIADLEIDLNNASSGFVGXXXXXX 540 *F SSKVMGNTQKVLVVAMFVWNQLNILLNFRRLARIYDDIADLEIDLNNASSGFVG *F Sbjct: 481 SSKVMGNTQKVLVVAMFVWNQLNILLNFRRLARIYDDIADLEIDLNNASSGFVGQRHWWR 540 *F Query: 541 XXXXXXXXXXXWIVLLVGLTPRFTLVALGPYLHWTNKVLTEIILIMLQLKCTEYCVFVLL 600 *F WIVLLVGLTPRFTLVALGPYLHWTNKVLTEIILIMLQLKCTEYCVFVLL *F Sbjct: 541 FRFRLALSVGLWIVLLVGLTPRFTLVALGPYLHWTNKVLTEIILIMLQLKCTEYCVFVLL 600 *F Query: 601 IYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQLLAGRIWGLVNEVXXXXXXXX 660 *F IYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQLLAGRIWGLVNEV *F Sbjct: 601 IYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQLLAGRIWGLVNEVSLYFTLSL 660 *F Query: 661 XXXXXXNELTILQIVNWALIKSVNPNECCQYTEDYLILKMGLREYSLQMEHLKLIFTCGG 720 *F NELTILQIVNWALIKSVNPNECCQYTEDYLILKMGLREYSLQMEHLKLIFTCGG *F Sbjct: 661 TLLFLYNELTILQIVNWALIKSVNPNECCQYTEDYLILKMGLREYSLQMEHLKLIFTCGG 720 *F Query: 721 LFDINLKFFGGVKLKL 736 *F LFDINLKFFGGVKLKL *F Sbjct: 721 LFDINLKFFGGVKLKL 736 *F Query= Gr98A2 , 294 bases, 14976701 checksum. *F (294 letters) *F Database: /data/blast/db/aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG12884|FBan0012884|CT32028|FBan0012884 last_updated:000321 918 1.5e-93 1 *F CG11769|FBan0011769|CT34710|FBan0011769 last_updated:000321 64 0.99 1 *F CG5625|FBan0005625|CT16940|FBan0005625 last_updated:000321 60 0.990 2 *F CG5625|FBan0005625|CT31493|FBan0005625 last_updated:000321 60 0.990 2 *F CG13976|FBan0013976|CT33530|FBan0013976 last_updated:000321 63 0.998 1 *F >CG12884|FBan0012884|CT32028|FBan0012884 last_updated:000321 *F Length = 736 *F Score = 918 (328.2 bits), Expect = 1.5e-93, P = 1.5e-93 *F Identities = 170/294 (57%), Positives = 220/294 (74%) *F Query: 1 MEAKRSRLLTTARPYLQVLSLFGLTPPAEXXXXXXXXXXXXCWMAGYSLYLIAILLMVFY 60 *F MEA RSRLL ARPY+Q+ S+FGLTPP \+ \+ GY+ YLI+I LMV Y *F Sbjct: 401 MEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIY 460 *F Query: 61 EFHANIVSLHLEIYKFHVEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIAN 120 *F E \+ANIV+L \+I+KFH ED SKVMG TQK L+VA+ NQLNILLN+ RL IYD+IA+ *F Sbjct: 461 ECYANIVALQKDIHKFHAEDSSKVMGNTQKVLVVAMFVWNQLNILLNFRRLARIYDDIAD 520 *F Query: 121 LDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVIIIGVIPRLTLGRAGPFFHWVNQVLT 180 *F L++ \++ \+S F G+ HWW FR RL LS+GLW+V+++G+ PR TL GP+ HW N+VLT *F Sbjct: 521 LEIDLNNASSGFVGQRHWWRFRFRLALSVGLWIVLLVGLTPRFTLVALGPYLHWTNKVLT 580 *F Query: 181 QIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQL 240 *F \+IILIMLQLK EYC+FVLL+YELILR RH+L+Q+ \+LE \+QELCV LK+NQL *F Sbjct: 581 EIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQL 640 *F Query: 241 LIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE 294 *F L GRIW LV+E+ YF S+TLLFLYN LTIL \+VNWA+I+S++PN+CCQ \+E+ *F Sbjct: 641 LAGRIWGLVNEVSLYFTLSLTLLFLYNELTILQIVNWALIKSVNPNECCQYTED 694 *F Score = 711 (255.3 bits), Expect = 1.3e-71, P = 1.3e-71 *F Identities = 136/294 (46%), Positives = 197/294 (67%) *F Query: 1 MEAKRSRLLTTARPYLQVLSLFGLTPPAEXXXXXXXXXXXXCWMAGYSLYLIAILLMVFY 60 *F M A++SRLL A PYL \+ S+F LTPP \+ M GY Y AIL VF *F Sbjct: 1 MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI 60 *F Query: 61 EFHANIVSLHLEIYKFHVEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIAN 120 *F \+ NI+++ E+ \+++V DF++VMG QK L \+A N LN+L+NY RLG IY \+IA+ *F Sbjct: 61 VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIAD 120 *F Query: 121 LDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVIIIGVIPRLTLGRAGPFFHWVNQVLT 180 *F L++ \+D++S+ F G+ \+SFR R+ L \+G+WM++++G \+PRLT+ GPF \+ \++LT *F Sbjct: 121 LEMDMDEASQCFGGQRQRFSFRFRMALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT 180 *F Query: 181 QIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQL 240 *F \+ \++IM QLK EYC+FVL++YEL+LR R L QL+++ \+D \+ \+Q LCV LK+NQL *F Sbjct: 181 EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL 240 *F Query: 241 LIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE 294 *F L+GRIWRL \++G+YF \+M LLFLYNGLTILH+VNWA I \+ CCQ E *F Sbjct: 241 LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYGPE 294 *F Score = 541 (195.5 bits), Expect = 1.3e-53, P = 1.3e-53 *F Identities = 101/101 (100%), Positives = 101/101 (100%) *F Query: 191 GPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVD 250 *F GPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVD *F Sbjct: 292 GPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVD 351 *F Query: 251 EIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL 291 *F EIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL *F Sbjct: 352 EIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL 392 *F \------------------------------------------------------------------------------ *F Query= GR98B.1,AC007817,45506-46916, 1411 bases, AE6EC367 checksum. *F (1411 letters) *F Translating both strands of query sequence in all 6 reading frames *F Database: /data/blast/db/aa_gadfly.dros *F 14,080 sequences; 6,850,524 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F CG12884|FBan0012884|CT32028|FBan0012884 last_updated:000321 1266 1.2e-129 1 *F CG15174|FBan0015174|CT35084|FBan0015174 last_updated:000321 74 0.53 1 *F CG10016|FBan0010016|CT28211|FBan0010016 last_updated:000321 57 0.82 1 *F CG17945|FBan0017945|CT39980|FBan0017945 last_updated:000321 55 0.93 1 *F >CG12884|FBan0012884|CT32028|FBan0012884 last_updated:000321 *F Length = 736 *F Plus Strand HSPs: *F Score = 1266 (450.7 bits), Expect = 1.2e-129, P = 1.2e-129 *F Identities = 255/300 (85%), Positives = 260/300 (86%), Frame = \+1 *F Query: 1 MEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIY 180 *F MEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIY *F Sbjct: 401 MEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIY 460 *F Query: 181 ECYANIVALQKDIHKFHAEDSSKVMGEYAERSWWVAMFVWNQLNILLNFRRLARIYDDIA 360 *F ECYANIVALQKDIHKFHAEDSSKVMG \++ VAMFVWNQLNILLNFRRLARIYDDIA *F Sbjct: 461 ECYANIVALQKDIHKFHAEDSSKVMGN-TQKVLVVAMFVWNQLNILLNFRRLARIYDDIA 519 *F Query: 361 DLEIDLNNASSGFVGXXXXXXXXXXXXXXXXXWIVLLVGLTPRFTLVALGPYLHWTNKVL 540 *F DLEIDLNNASSGFVG WIVLLVGLTPRFTLVALGPYLHWTNKVL *F Sbjct: 520 DLEIDLNNASSGFVGQRHWWRFRFRLALSVGLWIVLLVGLTPRFTLVALGPYLHWTNKVL 579 *F Query: 541 TEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQ 720 *F TEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQ *F Sbjct: 580 TEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQ 639 *F Query: 721 LLAGRIWGLVNEVXXXXXXXXXXXXXXNELTILQIVNWALIKSVNPNECCQYSK--LVFK 894 *F LLAGRIWGLVNEV NELTILQIVNWALIKSVNPNECCQY++ L+ K *F Sbjct: 640 LLAGRIWGLVNEVSLYFTLSLTLLFLYNELTILQIVNWALIKSVNPNECCQYTEDYLILK 699 *F Score = 625 (225.1 bits), Expect = 1.0e-84, Sum P(2) = 1.0e-84 *F Identities = 128/292 (43%), Positives = 176/292 (60%), Frame = \+1 *F Query: 1 MEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIY 180 *F M A \+SRLLA A PY+ I+S+F LTPP Q F T H+R R \++ GY Y \+I V *F Sbjct: 1 MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI 60 *F Query: 181 ECYANIVALQKDIHKFHAEDSSKVMGEYAERSWWVAMFVWNQLNILLNFRRLARIYDDIA 360 *F Y NI+A+ \+++ \+++ D \++VMG \++S \+ M \+ N LN+L+N+RRL IY DIA *F Sbjct: 61 VSYFNIIAIDEEVLEYNVSDFTRVMGNI-QKSLYSIMAIANHLNMLINYRRLGGIYKDIA 119 *F Query: 361 DLEIDLNNASSGFVGXXXXXXXXXXXXXXXXXWIVLLVGLTPRFTLVALGPYLHWTNKVL 540 *F DLE+D++ AS F G W++L+VG PR T+ A+GP++ K+L *F Sbjct: 120 DLEMDMDEASQCFGGQRQRFSFRFRMALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKIL 179 *F Query: 541 TEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQ 720 *F TE \++IM QLK EYCVFVL+IYEL+LR R L Q+ E \+ \+ \+D \+Q LCVALKRNQ *F Sbjct: 180 TEFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQ 239 *F Query: 721 LLAGRIWGLVNEVXXXXXXXXXXXXXXNELTILQIVNWALIKSVNPNECCQY 876 *F LL GRIW L \+V N LTIL \+VNWA I \+ CCQY *F Sbjct: 240 LLLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQY 291 *F Score = 290 (107.1 bits), Expect = 4.5e-44, Sum P(2) = 4.5e-44 *F Identities = 56/109 (51%), Positives = 73/109 (66%), Frame = \+1 *F Query: 580 EYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKRNQLLAGRIWGLVNEV 759 *F EYC+FVLL+YELILR RH+L+Q+ \+LE \+QELCV LK+NQLL GRIW LV+E+ *F Sbjct: 294 EYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVDEI 353 *F Query: 760 XXXXXXXXXXXXXXNELTILQIVNWALIKSVNPNECCQYSKLVFKFKRN 906 *F N LTIL \+VNWA+I+S++PN+CCQ \+ F F N *F Sbjct: 354 GAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL--MSFHFSLN 400 *F Score = 236 (88.1 bits), Expect = 1.0e-84, Sum P(2) = 1.0e-84 *F Identities = 50/78 (64%), Positives = 54/78 (69%), Frame = \+2 *F Query: 1019 VYLYNSLKCXXXXXXXXXNSISRVLHQMYCLSAAEDYLILKMGLREYSLQMEHLKLIFTC 1198 *F \++LYN L S++ C EDYLILKMGLREYSLQMEHLKLIFTC *F Sbjct: 663 LFLYNELTILQIVNWALIKSVN----PNECCQYTEDYLILKMGLREYSLQMEHLKLIFTC 718 *F Query: 1199 GGLFDINLKFFGGVKLKL 1252 *F GGLFDINLKFFGGVKLKL *F Sbjct: 719 GGLFDINLKFFGGVKLKL 736 *F \------------------------------------------------------------------------------ *F Query= Gr98A1 , 736 bases, BF190611 checksum. *F (736 letters) *F Database: /data/blast/db/na_geno.dros *F 1181 sequences; 122,680,987 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F Reading High Probability *F Sequences producing High-scoring Segment Pairs: Frame Score P(N) N *F gadfly|SEG:AE003761|gb|AE003761|Drosophila melanogaste... \+2 1389 0.0 4 *F >gadfly|SEG:AE003761|gb|AE003761.1|Drosophila melanogaster genomic scaffold *F GI:7301591 *F Length = 224,614 *F Plus Strand HSPs: *F Score = 1389 (494.0 bits), Expect = 0.0, Sum P(4) = 0.0 *F Identities = 272/292 (93%), Positives = 272/292 (93%), Frame = \+2 *F Query: 1 MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI 60 *F MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI *F Sbjct: 106004 MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFI *F 106183 *F Query: 61 VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIAX 120 *F VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIA *F Sbjct: 106184 VSYFNIIAIDEEVLEYNVSDFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIAD *F 106363 *F Query: 121 XXXXXXEASQCXXXXXXXXXXXXXMALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT 180 *F EASQC MALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT *F Sbjct: 106364 LEMDMDEASQCFGGQRQRFSFRFRMALCVGVWMILMVGSMPRLTMTAMGPFVSTLLKILT *F 106543 *F Query: 181 EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL 240 *F EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL *F Sbjct: 106544 EFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKRNQL *F 106723 *F Query: 241 LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYG 292 *F LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYG *F Sbjct: 106724 LLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYG 106879 *F Score = 1350 (480.3 bits), Expect = 0.0, Sum P(4) = 0.0 *F Identities = 269/307 (87%), Positives = 273/307 (88%), Frame = \+2 *F Query: 393 MSFHFSLNMEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLI 452 *F \+SFHFSLNMEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLI *F Sbjct: 109535 VSFHFSLNMEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLI *F 109714 *F Query: 453 SISLMVIYECYANIVALQKDIHKFHAEDSSKVMGNTQKVLVVAMFVWNQLNILLNFRRLA 512 *F SISLMVIYECYANIVALQKDIHKFHAEDSSKVMGNTQKVLVVAMFVWNQLNILLNFRRLA *F Sbjct: 109715 SISLMVIYECYANIVALQKDIHKFHAEDSSKVMGNTQKVLVVAMFVWNQLNILLNFRRLA *F 109894 *F Query: 513 RIYDDIADLEIDLNNASSGFVGXXXXXXXXXXXXXXXXXWIVLLVGLTPRFTLVALGPYL 572 *F RIYDDIADLEIDLNNASSGFVG WIVLLVGLTPRFTLVALGPYL *F Sbjct: 109895 RIYDDIADLEIDLNNASSGFVGQRHWWRFRFRLALSVGLWIVLLVGLTPRFTLVALGPYL *F 110074 *F Query: 573 HWTNKVLTEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELC 632 *F HWTNKVLTEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELC *F Sbjct: 110075 HWTNKVLTEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELC *F 110254 *F Query: 633 VALKRNQLLAGRIWGLVNEVXXXXXXXXXXXXXXNELTILQIVNWALIKSVNPNECCQYT 692 *F VALKRNQLLAGRIWGLVNEV NELTILQIVNWALIKSVNPNECCQY+ *F Sbjct: 110255 VALKRNQLLAGRIWGLVNEVSLYFTLSLTLLFLYNELTILQIVNWALIKSVNPNECCQYS *F 110434 *F Query: 693 EDYLILK 699 *F \+ L+ K *F Sbjct: 110435 K--LVFK 110449 *F Score = 541 (195.5 bits), Expect = 0.0, Sum P(4) = 0.0 *F Identities = 101/101 (100%), Positives = 101/101 (100%), Frame = \+1 *F Query: 292 GPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVD 351 *F GPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVD *F Sbjct: 108526 GPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQLLIGRIWRLVD *F 108705 *F Query: 352 EIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL 392 *F EIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL *F Sbjct: 108706 EIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQL 108828 *F \------- *F Query= Gr98A2 , 294 bases, 14976701 checksum. *F (294 letters) *F Database: /data/blast/db/na_geno.dros *F 1181 sequences; 122,680,987 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F Reading High Probability *F Sequences producing High-scoring Segment Pairs: Frame Score P(N) N *F gadfly|SEG:AE003761|gb|AE003761|Drosophila melanogaste... \+1 1470 2.4e-149 1 *F gadfly|SEG:AE003803|gb|AE003803|Drosophila melanogaste... \-1 86 0.85 1 *F >gadfly|SEG:AE003761|gb|AE003761.1|Drosophila melanogaster genomic scaffold *F 142000013386035 section 86 of 105, complete sequence.|AE003761.1 *F GI:7301591 *F Length = 224,614 *F Plus Strand HSPs: *F Score = 1470 (522.5 bits), Expect = 2.4e-149, P = 2.4e-149 *F Identities = 282/294 (95%), Positives = 282/294 (95%), Frame = \+1 *F Query: 1 MEAKRSRLLTTARPYLQVLSLFGLTPPAEXXXXXXXXXXXXCWMAGYSLYLIAILLMVFY 60 *F MEAKRSRLLTTARPYLQVLSLFGLTPPAE CWMAGYSLYLIAILLMVFY *F Sbjct: 107956 MEAKRSRLLTTARPYLQVLSLFGLTPPAEFFTRTLRKRRRFCWMAGYSLYLIAILLMVFY *F 108135 *F Query: 61 EFHANIVSLHLEIYKFHVEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIAN 120 *F EFHANIVSLHLEIYKFHVEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIAN *F Sbjct: 108136 EFHANIVSLHLEIYKFHVEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIAN *F 108315 *F Query: 121 LDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVIIIGVIPRLTLGRAGPFFHWVNQVLT 180 *F LDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVIIIGVIPRLTLGRAGPFFHWVNQVLT *F Sbjct: 108316 LDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVIIIGVIPRLTLGRAGPFFHWVNQVLT *F 108495 *F Query: 181 QIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQL 240 *F QIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQL *F Sbjct: 108496 QIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTLKQNQL *F 108675 *F Query: 241 LIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE 294 *F LIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE *F Sbjct: 108676 LIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLSEE 108837 *F \--------- *F OK *F BLASTN 2.0MP-WashU 09-Sep-1999 sol2.6-ultra 12:35:56 09-Sep-1999 *F Copyright (C) 1996-1999 Washington University, Saint Louis, Missouri USA. *F All Rights Reserved. *F Reference: Gish, W. (1996-1999) http://blast.wustl.edu *F Notice: this program and its default parameter settings are optimized to find *F nearly identical sequences rapidly. To identify weak similarities encoded in *F nucleic acid, use BLASTX, TBLASTN or TBLASTX. *F Query= GR98B.1,AC007817,45506-46916, 1411 bases, AE6EC367 checksum. *F (1411 letters) *F Database: /data/blast/db/na_geno.dros *F 1181 sequences; 122,680,987 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F gadfly|SEG:AE003761|gb|AE003761.1|Drosophila melanogaster... 7010 1.7e-310 1 *F gadfly|SEG:AE003680|gb|AE003680.1|Drosophila melanogaster... 229 0.29 1 *F gadfly|SEG:AE003843|gb|AE003843.1|Drosophila melanogaster... 219 0.62 1 *F gadfly|SEG:AE003528|gb|AE003528.1|Drosophila melanogaster... 217 0.70 1 *F gadfly|SEG:AE003703|gb|AE003703.1|Drosophila melanogaster... 211 0.90 1 *F gadfly|SEG:AE003796|gb|AE003796.1|Drosophila melanogaster... 207 0.97 1 *F gadfly|SEG:AE003655|gb|AE003655.1|Drosophila melanogaster... 206 0.98 1 *F gadfly|SEG:AE003604|gb|AE003604.1|Drosophila melanogaster... 203 0.995 1 *F gadfly|SEG:AE003826|gb|AE003826.1|Drosophila melanogaster... 203 0.995 1 *F gadfly|SEG:AE003530|gb|AE003530.1|Drosophila melanogaster... 203 0.995 1 *F gadfly|SEG:AE003534|gb|AE003534.1|Drosophila melanogaster... 200 0.9993 1 *F gadfly|SEG:AE003539|gb|AE003539.1|Drosophila melanogaster... 197 0.99995 1 *F gadfly|SEG:AE003840|gb|AE003840.1|Drosophila melanogaster... 197 0.99995 1 *F >gadfly|SEG:AE003761|gb|AE003761.1|Drosophila melanogaster genomic scaffold *F 142000013386035 section 86 of 105, complete sequence.|AE003761.1 *F GI:7301591 *F Length = 224,614 *F Plus Strand HSPs: *F Score = 7010 (1057.8 bits), Expect = 1.7e-310, P = 1.7e-310 *F Identities = 1408/1411 (99%), Positives = 1408/1411 (99%), Strand = Plus / Plus *F Query: 1 ATGGAAGCCAATCGGAGTCGTCTGCTGGCCGCAGCGCGTCCTTACATTCAGATTTATTCC 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109559 ATGGAAGCCAATCGGAGTCGTCTGCTGGCCGCAGCGCGTCCTTACATTCAGATTTATTCC *F 109618 *F Query: 61 ATTTTCGGACTCACGCCGCCAATTCAGTTTTTTACCAGGACCTTACATAAGCGACGTAGA 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109619 ATTTTCGGACTCACGCCGCCAATTCAGTTTTTTACCAGGACCTTACATAAGCGACGTAGA *F 109678 *F Query: 121 GGAATTGTGATATTGGGCTACGCCTGCTATTTAATTAGCATTTCCCTGATGGTCATCTAT 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109679 GGAATTGTGATATTGGGCTACGCCTGCTATTTAATTAGCATTTCCCTGATGGTCATCTAT *F 109738 *F Query: 181 GAGTGCTACGCGAACATTGTGGCTCTGCAAAAGGATATACATAAGTTTCACGCCGAGGAC 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109739 GAGTGCTACGCGAACATTGTGGCTCTGCAAAAGGATATACATAAGTTTCACGCCGAGGAC *F 109798 *F Query: 241 TCTAGCAAAGTTATGGGGGAATACGCAGAAAGGTCCTGGTGGGTAGCCATGTTCGTTTGG 300 *F |||||||||||||||||| ||||||||||| ||||||||||| ||||||||||||||||| *F Sbjct: 109799 TCTAGCAAAGTTATGGGG-AATACGCAGAA-GGTCCTGGTGG-TAGCCATGTTCGTTTGG *F 109855 *F Query: 301 AATCAATTGAACATTCTGCTTAACTTTCGGCGCCTTGCTAGGATTTATGATGATATTGCG 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109856 AATCAATTGAACATTCTGCTTAACTTTCGGCGCCTTGCTAGGATTTATGATGATATTGCG *F 109915 *F Query: 361 GATCTGGAAATAGATTTGAATAACGCCTCTAGCGGTTTTGTTGGCCAACGGCACTGGTGG 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109916 GATCTGGAAATAGATTTGAATAACGCCTCTAGCGGTTTTGTTGGCCAACGGCACTGGTGG *F 109975 *F Query: 421 CGCTTCCGTTTCCGGTTGGCCCTCTCTGTGGGCCTGTGGATAGTGTTGCTGGTGGGTCTC 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 109976 CGCTTCCGTTTCCGGTTGGCCCTCTCTGTGGGCCTGTGGATAGTGTTGCTGGTGGGTCTC *F 110035 *F Query: 481 ACGCCACGATTCACCCTCGTGGCACTCGGACCCTACCTCCACTGGACAAATAAAGTGCTC 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110036 ACGCCACGATTCACCCTCGTGGCACTCGGACCCTACCTCCACTGGACAAATAAAGTGCTC *F 110095 *F Query: 541 ACCGAAATCATTCTGATAATGCTACAACTTAAGTGTACAGAGTATTGTGTGTTTGTGCTC 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110096 ACCGAAATCATTCTGATAATGCTACAACTTAAGTGTACAGAGTATTGTGTGTTTGTGCTC *F 110155 *F Query: 601 CTGATCTATGAACTGATCCTCCGAGGGCGCCACATCCTTCAGCAGATCAGTGTGGAGCTC 660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110156 CTGATCTATGAACTGATCCTCCGAGGGCGCCACATCCTTCAGCAGATCAGTGTGGAGCTC *F 110215 *F Query: 661 GAGGGTAACCAGTCAAGGGACAGTGTTCAGGAGCTGTGTGTGGCCTTGAAACGCAATCAG 720 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110216 GAGGGTAACCAGTCAAGGGACAGTGTTCAGGAGCTGTGTGTGGCCTTGAAACGCAATCAG *F 110275 *F Query: 721 TTGCTGGCTGGACGCATTTGGGGCTTGGTGAATGAGGTCAGCTTGTATTTTACCCTATCC 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110276 TTGCTGGCTGGACGCATTTGGGGCTTGGTGAATGAGGTCAGCTTGTATTTTACCCTATCC *F 110335 *F Query: 781 TTGACGCTTTTGTTTCTCTACAATGAACTGACCATTCTGCAAATTGTCAATTGGGCTCTC 840 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110336 TTGACGCTTTTGTTTCTCTACAATGAACTGACCATTCTGCAAATTGTCAATTGGGCTCTC *F 110395 *F Query: 841 ATTAAATCCGTCAATCCAAACGAATGCTGTCAATATAGTAAGTTAGTTTTCAAGTTCAAA 900 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110396 ATTAAATCCGTCAATCCAAACGAATGCTGTCAATATAGTAAGTTAGTTTTCAAGTTCAAA *F 110455 *F Query: 901 AGAAACTTTACCTATAAACAAGTTATTTTCATAATAGGGCGCGTTGGTACTTGCCTCTTG 960 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110456 AGAAACTTTACCTATAAACAAGTTATTTTCATAATAGGGCGCGTTGGTACTTGCCTCTTG *F 110515 *F Query: 961 CTGTCAATCAATATTTTTCTATCCTGTTTATACAGCGAGTTCTGCATTCAAACAGTAAGT 1020 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110516 CTGTCAATCAATATTTTTCTATCCTGTTTATACAGCGAGTTCTGCATTCAAACAGTAAGT *F 110575 *F Query: 1021 ATACCTCTATAATTCTTTAAAATGTTTTTAATAATTTTATTTTTATAAGTATAATAGCAT 1080 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110576 ATACCTCTATAATTCTTTAAAATGTTTTTAATAATTTTATTTTTATAAGTATAATAGCAT *F 110635 *F Query: 1081 TTCACGAGTTCTTCACCAAATGTATTGCCTTTCTGCAGCCGAAGATTATCTAATATTAAA 1140 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110636 TTCACGAGTTCTTCACCAAATGTATTGCCTTTCTGCAGCCGAAGATTATCTAATATTAAA *F 110695 *F Query: 1141 AATGGGCCTGAGGGAATACTCGCTGCAAATGGAGCATTTAAAGCTGATTTTCACATGCGG 1200 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110696 AATGGGCCTGAGGGAATACTCGCTGCAAATGGAGCATTTAAAGCTGATTTTCACATGCGG *F 110755 *F Query: 1201 TGGCCTCTTTGACATCAATCTTAAGTTCTTCGGAGGGGTAAAACTTAAATTATAAATTAA 1260 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110756 TGGCCTCTTTGACATCAATCTTAAGTTCTTCGGAGGGGTAAAACTTAAATTATAAATTAA *F 110815 *F Query: 1261 AAATCAAGCTTATGTACACTTTCCTTTCTTCTAGATGGTAGTCACCTTATTCGGTTATAT 1320 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110816 AAATCAAGCTTATGTACACTTTCCTTTCTTCTAGATGGTAGTCACCTTATTCGGTTATAT *F 110875 *F Query: 1321 CATTATTCTCGTGCAATTTAAAATTCAATTTTTTGCTCAATCAAATTTTATGCAAAATAT 1380 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 110876 CATTATTCTCGTGCAATTTAAAATTCAATTTTTTGCTCAATCAAATTTTATGCAAAATAT *F 110935 *F Query: 1381 TAACAGCACCGAACTGAAAGCATATACCGCG 1411 *F ||||||||||||||||||||||||||||||| *F Sbjct: 110936 TAACAGCACCGAACTGAAAGCATATACCGCG 110966 # *U FBrf0137494 *a Mazzarelli *b J. *t 2001.8.15 *T personal communication to FlyBase *u FlyBase error report for CG11427 on Wed Aug 15 08:48:06 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 15 Aug 2001 08:48:06 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: mazz@snowball.pcbi.upenn.edu *F Subject: FlyBase error report for CG11427 on Wed Aug 15 08:48:06 2001 *F Error report from Joan Mazzarelli (mazz@pcbi.upenn.edu) *F Gene or accession: CG11427 *F Release: 1 *F Gene annotation error *F Gene CG11427 has a mistake in the supporting evidence or functional assignment. *F Comments: GO molecular function annotation as chaperone should be changed to *F vesicle transporter *F based on protein similarity to adaptin # *U FBrf0137495 *a Green *b R.B. *t 2001.8.15 *T personal communication to FlyBase *u FlyBase error report for CG10016 on Wed Aug 15 17:15:03 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 15 Aug 2001 17:15:04 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: rbgreen@ucla.edu *F Subject: FlyBase error report for CG10016 on Wed Aug 15 17:15:03 2001 *F Error report from Ryan B. Green (rbgreen@ucla.edu) *F Gene or accession: CG10016 *F Release: 1 *F Gene annotation error *F Genes CG10016 and drm should be merged. *F Comments: We have molecularly characterized the mutations in all drm alleles *F and find that they all map to the CG10016 gene. Therefore, CG10016 should be *F linked to the drm gene. # *U FBrf0137496 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG5659 on Thu Aug 16 15:03:08 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:03:08 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG5659 on Thu Aug 16 15:03:08 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG5659 *F Release: 1 *F Gene annotation error *F Gene CG5659 has incorrect exon/intron structure or translation start site. *F Comments: ari-1 shows a good match (66%) to part of the 1360 element from *F 2853-3537 of its 4383bp. *F Query= ari-1|FBgn0017418|CT17874|FBan0005659 GO: located on: X 17A1-17A1; *F |cDNA sequence *F (4383 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|M55078|1360 DMIS1360 1177bp AKA(M38659) Derived from M... 1017 5.4e-49 *F 2 gb|X59545|mdg1 DMRTMGD1 7480bp Derived from X59545 (g8507... 275 2.4e-05 *F 1 FB|FBgn0001207|HMS-Beagle Beagle 7062bp 274 2.6e-05 *F 1 gb|U06920|HeT-A DM06920 6943bp Derived from U06920 (g1871... 245 *F 0.00054 1 gb|X01472|17.6 DMIS176 7439bp AKA(J01060,J01061) Derived ... 240 *F 0.00092 1 FB|FBgn0020938|yoyo DM_YOYO 7521bp *F 232 0.0021 1 gb|AF364550|cruiser CRUISER 7387bp Derived from P1 clones... *F 223 0.0062 2 gb|AJ000387|ZAM DMZAM 8435bp Derived from AJ000387 (e1237... *F 213 0.016 1 gb|X04132|412 DMRT412G 6897bp AKA(X03733) Derived from *F X0... 202 0.048 1 gb|AJ009736|Idefix DME9736 7411bp Derived from *F AJ009736 (... 191 0.14 1 FB|FBgn0000155|roo DM_ROO 9092bp *F 186 0.23 1 gb|X07656|1731 DMTN1731 4648bp Derived from *F X07656 (g8700... 185 0.25 1 gb|U14101|TART-element DM1410! *F 1 10654bp Derived from U1410... 192 0.31 2 gb|AF315785|midline 4617bp *F Derived from AF315785.1 (Rel. ... 176 0.52 1 gb|X80025|hopper DMTRDNA *F 1435bp Derived from X80025 (g510... 167 0.80 1 gb|X05643|D-element *F DMTPOD 408bp Derived from X05643 (g87... 156 0.88 1 gb|M12927|gypsy *F DMGYPF1A 7469bp Derived from M12927 (g157... 160 0.98 1 *F gb|AC005847|Waldo-B WALDOB 5180bp 153 0.9997 *F 1 gb|X67681|Bari1 DMBARI1 1728bp AKA(S55767) Derived from X... 151 0.9999 *F 1> *F >gb|M55078|1360 DMIS1360 1177bp AKA(M38659) Derived from M55078 (g157768) *F (Rel. 30, Last updated, Version 2). *F Length = 1177 *F Plus Strand HSPs: *F Score = 1017 (158.6 bits), Expect = 5.4e-49, Sum P(2) = 5.4e-49 *F Identities = 449/675 (66%), Positives = 449/675 (66%), Strand = Plus / Plus *F Query: 2853 CAAAGACAATAGAATAACAAGATGCGTAACGCAACGCGATTTTTTAGCACACATTTTTTT 2912 *F |||||||| || ||||| ||||||| |||||| | ||||||||| | | | | *F Sbjct: 1 CAAAGACACTAAAATAATAAGATGCATAACGCCATACGATTTTTTCGT-CGTGGCTCTAG 59 *F Query: 2913 GGCAATGGATCTAGAAGGTGGCTCCAGGCTCTCTC-GAATTTTTGAGA-GAGAGCGAGAG 2970 *F || ||| || | | | ||| | | | | || ||| | ||||| | *F Sbjct: 60 AGCG-TGGCTCCA--AC-TCTCTCGAATTTTTGTTAGAGAGCGAGAGGCGGGAGCGCTAC 115 *F Query: 2971 AGTGGAGAGCGCTA--CAGCAAATAGCTCTTTTCTACGTATACAG-TGATAGCAGA-CAA 3026 *F || | | ||| || | || ||| | | || | | |||| || || | || *F Sbjct: 116 AGCGAACAGCTCTTTTCTGCGCATA---CAGTGATA-GCAGACAGCTG-TATGTGTGCAC 170 *F Query: 3027 TTGTATG-TG-TGCACACGTATGCTT-ATGCATTGTAAATTTGACCCCC----TTCTTAG 3079 *F ||||| | |||| ||| || | | | | || ||| | ||||||| *F Sbjct: 171 ACGTATGCTCATGCATT-GTAAATTTGACAAAATATGCCCTTCACCTCAGAAGTTCTTAG 229 *F Query: 3080 ACTTTAAATCTTTATTATTTTTGATCAATTGGCACCATGCTAAAAATTCTTGTTTTGCAT 3139 *F ||||||||||| |||||||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 230 ACTTTAAATCTATATTATTTTTGATCAATTGGCACCATGCGAAAAATTCTTGTTTTGCAT 289 *F Query: 3140 TGCCTTAACGTTATTATTATTTGAAAATAGATTAGAAATAGCCAAATCTATGTACATATT 3199 *F |||||||||||||||||||||| ||||||||||||||||||| ||||||||||||||||| *F Sbjct: 290 TGCCTTAACGTTATTATTATTTCAAAATAGATTAGAAATAGCTAAATCTATGTACATATT 349 *F Query: 3200 ATCACAAAATAAATTTCAAAAATTACTTTATTAGAATATTTGTCATTAGAGTATTCAGCT 3259 *F |||||||||||||||| |||||| ||| ||| ||||||||||||||||||||||| | || *F Sbjct: 350 ATCACAAAATAAATTTAAAAAATGACTATATAAGAATATTTGTCATTAGAGTATTGATCT 409 *F Query: 3260 TGCGGCGTGTGAAAAATTAAATTTTTGTAA-AATT--T-A-TG-TT-T-TAA--ATTACG 3309 *F |||| ||||||||||| ||| || | || |||| | | || || | | | | || *F Sbjct: 410 TGCGTCGTGTGAAAAAATAA-TTAG-GCAATAATTGTTGAGTGCTTGTGTCGCCACTTCG 467 *F Query: 3310 GTAGTTAATATAATTTGCTTTGTATTGAGTATATTTATTAAGTCATTTGACTTA-ATATG 3368 *F | || | ||| | | | || || || || || || ||| ||||| *F Sbjct: 468 TGCCTCAAGA-AATGACCAAAGCAAAGACACTAGA-AT-AATTCTACTGTCTTTGATATG 524 *F Query: 3369 ATGTATGTAAATGTACCATTTCAATTATTTAAA-ATGCACATTAGATTCAGTTGTTTTTC 3427 *F | | || || | ||| || | | |||| || || | ||| | || ||| | *F Sbjct: 525 ACTTTTGCAA-TAAACCGTTATA--TGTTTATTTATTTT-ATAA-ATTTA-TT-TTTATG 577 *F Query: 3428 AGTTTTTGTTTTTTTTTTTTTTTTGTATTCCAATTTGATATTTTTTCATCATATTGCTAG 3487 *F | | || | ||| | | ||| ||| || || | ||| | | | ||| | || *F Sbjct: 578 AAATATT-TATTTCTCAACGTAATGTCTTCTTTTTAGAAAATTTATGA--A-ATTAC-AG 632 *F Query: 3488 T-GCCAAACCAATGT 3501 *F | || | | ||| | *F Sbjct: 633 TAGCTATAATAATTT 647 *F Score = 228 (40.3 bits), Expect = 5.4e-49, Sum P(2) = 5.4e-49 *F Identities = 52/57 (91%), Positives = 52/57 (91%), Strand = Plus / Plus *F Query: 3482 TGC-TAGTGCCAAACCAATGTAAGGCCGTTGCGCATCTTGTTATTCTAGTGTCTTTG 3537 *F ||| |||||||||||||||||| ||| ||| ||||||||||||||||||||||||| *F Sbjct: 1122 TGCATAGTGCCAAACCAATGTATGGC-GTTATGCATCTTGTTATTCTAGTGTCTTTG 1177 *F Score = 214 (38.2 bits), Expect = 2.9e-07, Sum P(2) = 2.9e-07 *F Identities = 156/260 (60%), Positives = 156/260 (60%), Strand = Plus / Plus *F Query: 3186 TCTATGTACAT-ATTATCACAAAATAAATTTCAAAAATTACTT-TATTAGAATATTTGTC 3243 *F ||| || | || | | | ||| | | || | ||| || | ||| || |||| | *F Sbjct: 514 TCTTTG-ATATGACTTTTGCAATAAACCGTTATATGTTTATTTATTTTATAA-ATTTATT 571 *F Query: 3244 ATTA-GAG-TATTCAGCTTGCGGCGTGTGAAAAATTAAATTTTTGTAAAATT-T-ATGTT 3299 *F ||| || |||| | | | ||| | || |||| | ||| || | | || *F Sbjct: 572 TTTATGAAATATTTATTTCTCAACGTA--ATGTCTTCT-TTTTAGAAAATTTATGAAATT 628 *F Query: 3300 TTAA-AT-TACGGTAGTTAATATAAT--TTGCTTTG-T-ATTGAGTATATTTATTAAGTC 3353 *F | | || || || ||| | || ||| | ||| |||| |||||||||||| *F Sbjct: 629 ACAGTAGCTATAATAATTTCTATTGTACTTCCTTAAATTATTTAGTACATTTATTAAGTC 688 *F Query: 3354 ATTTGACTTAATATGATGTATGTAAATGTACCATTTCAATTATTTAAAATGCACATTA-G 3412 *F |||||||||||||| ||||| | || || ||| || | ||| ||| | ||| | *F Sbjct: 689 ATTTGACTTAATATCATGTA---ACAT-TAAGATT--AAGTGTTTCAAAAAAATATTTCG 742 *F Query: 3413 A-TTCAGTTGTTTTTCAGTT 3431 *F || | | || |||| | *F Sbjct: 743 CCTTTAAAAGATTGTCAGAT 762 *F Score = 146 (28.0 bits), Expect = 0.00025, Sum P(2) = 0.00025 *F Identities = 204/361 (56%), Positives = 204/361 (56%), Strand = Plus / Plus *F Query: 3150 TTATT-ATTATTTGAAAATAGATT-A-GAAATAGCCAAATCT--ATGTACA-TATTATCA 3203 *F ||||| ||| | | || || || | |||||| | ||| | ||| | || | *F Sbjct: 550 TTATTTATTTTATAAATTTATTTTTATGAAATATTTATTTCTCAACGTAATGTCTTCTTT 609 *F Query: 3204 CAAAATAAATTTCAAAAATTACTTTAT-TAGAATATTTGTC-ATTAGAGTAT-TCAGCTT 3260 *F | | |||||| ||||||| || || |||| || || ||| | | | | || *F Sbjct: 610 TTAGA-AAATTTATGAAATTACAGTAGCTATAATAATT-TCTATTGTACTTCCTTAAATT 667 *F Query: 3261 GCGGCGTGTGAAAAATTAAATT-TTTGTAA-AATTT-ATGTT---TTAA-ATTACGGTAG 3313 *F || | ||||| | |||| ||| | |||| |||| |||| | | *F Sbjct: 668 ATTTAGTAC-ATTTATTAAGTCATTTGACTTAATATCATGTAACATTAAGATTAAG-TGT 725 *F Query: 3314 TT-AATATAATTTGCTTTGTATTGAGTATATTTATTAAGTCATTTGACTTAATATGATGT 3372 *F || || | ||| | || | || | | ||| | | | || || || || | | *F Sbjct: 726 TTCAAAAAAATAT--TTCGCCTTTAAAAGATTG-TCA-G--ATGAGAGACAA-ATTA-GA 777 *F Query: 3373 ATGTAAATGTACCATTTCAATTATTTAAAATGCACATTAGA-TTCAGTT--GTTTTTCAG 3429 *F || |||| || || | ||| | |||| | | | | || ||| | |||| || *F Sbjct: 778 AT-TAAACATAA-ATATAAATG-TGTAAACGGTAGCTGATTCTTGAGTGCCGATTTTAAG 834 *F Query: 3430 TTT----TTGTTTTTTTTTTTTTTTTGTATTCCAATTTGATATTTTTTCATCATATTGCT 3485 *F ||| ||| ||| | || || || || || ||| ||||||||||| *F Sbjct: 835 AAACGAATTGCAAAAAGCTTTAATTTTT-TTAAAAATT-ATTTTATTTTATCATATTGCT 892 *F Query: 3486 A 3486 *F | *F Sbjct: 893 A 893 *F Minus Strand HSPs: *F Score = 268 (46.3 bits), Expect = 7.7e-06, Sum P(2) = 7.7e-06 *F Identities = 314/566 (55%), Positives = 314/566 (55%), Strand = Minus / Plus *F Query: 3397 AATAATTGAAATGGTACATTTACATACATCATATTAAGTCAAATGACTTAATAAATAT-A 3339 *F ||||||| || |||| || | || || || ||| || | | | |||| || | | *F Sbjct: 640 AATAATTTCTATTGTAC--TTCCTTAAATTAT-TTA-GTACATTTA-TTAAGTCATTTGA 694 *F Query: 3338 CTCAATACAAAGCAA-ATTAT-ATTAACTACCGTAATTTAAAACATAAATTTTACAAAAA 3281 *F || |||| | | || |||| ||||| | || || | | |||| ||| | *F Sbjct: 695 CTTAATATCATGTAACATTAAGATTAAGTGTTTCAAAAAAATATTTCGCCTTTA-AAAGA 753 *F Query: 3280 TT-TAATTTTTCACACGCCGCAAGCTGAATACTCTAATGACAAATATTCTAATAA-AG-T 3224 *F || | | | | || | | || || ||| | |||| | || || | *F Sbjct: 754 TTGTCAGATGAGAGACAAATTAGAATTAA-ACATAAAT-ATAAATGTGTAAACGGTAGCT 811 *F Query: 3223 AATTTTTGAAATTT-ATTTTGTGATAATA-T-GTACATAGATTTGGCTATTTCTAATCTA 3167 *F ||| |||| ||||| || | | | | | | || ||| | ||| || | *F Sbjct: 812 GATTCTTGAGTGCCGATTTTAAGAAACGAATTGCAAAAAGCTTTAATTTTTTTAAAAATT 871 *F Query: 3166 TTTTCAAATAATAATAACGTTAAGGCAATGCAAAACAAGAATTTTTAGCATGGTGCCAAT 3107 *F ||| | | || ||| | || | | || |||| || | | | ||| | | ||| *F Sbjct: 872 ATTTTATTTTATCATATTGCTACGAAATTGGAAAA-AACTACCCTAAT-ATG-TAC-AAT 927 *F Query: 3106 TGATCAAAAATAATAAA-GATTTAAAGTCTAAGAAGGGGGTCAAATTTACAATGCATAAG 3048 *F || || | ||| || | || || |||| | | | | *F Sbjct: 928 \--ATAAATTCGTTTCTTCGATCAGAATT-TATTCCGGCCCGAAAATCGTCTTTCTACCA- 983 *F Query: 3047 CATACGTGTGCACACATACAATTGTC-TGCTATCACTGTATACGTAGAAAAGAGCTATTT 2989 *F || || || |||| |||| ||| || || ||| | | | | ||||| *F Sbjct: 984 CA-ACATGCACACATATACGCG-GTCCTGGC-TC-CTGGCCCCATCCATACTAGCTA--- 1036 *F Query: 2988 GCTGTAGCGC-TCTCCACTCTCTCGCTCTCTC-TCAAAAATTCGAGAGAGCCTGGAGCCA 2931 *F |||| | | | ||| || |||| || | | ||| | ||| ||| || | *F Sbjct: 1037 GCTG-ATCCCATCTTCAGG-TCTC-CTATGGCCTCAGCGCGT-GAGCGAGTACAAAGAGA 1092 *F Query: 2930 CCT-TCTAGATCCATTGCCAAAAAAATGTGTGC-TAAA---AAATCGC-GT-TG-CGTTA 2879 *F || | | | || | | |||||| || ||| || ||| | || || ||||| *F Sbjct: 1093 GCTATTTTGG-CCGTCACTAAAAAAGTGGCTGCATAGTGCCAAACCAATGTATGGCGTTA 1151 *F Query: 2878 CGCATCTTGTTATTCTATTGTCTTTG 2853 *F |||||||||||||||| |||||||| *F Sbjct: 1152 TGCATCTTGTTATTCTAGTGTCTTTG 1177 *F Score = 148 (28.3 bits), Expect = 1.5, Sum P(2) = 0.77 *F Identities = 214/395 (54%), Positives = 214/395 (54%), Strand = Minus / Plus *F Query: 3520 CAAGATGCGCAACGGCCTTACATTGGTTTGGCACTAGCAATATGATGAAAA-AATATCAA 3462 *F || ||||| || ||| ||| ||| | || | ||| || | || || | *F Sbjct: 262 CACCATGCGAAAAATTCTTGTTTTGCATTGCCT-TAACGTTATTATTATTTCAAAAT-AG 319 *F Query: 3461 ATTGGAA-TA-CAAAAAAAAAAAAAAAACAAAAACTGAAAAACAACTGAATCTAATGTGC 3404 *F ||| ||| || | ||| | | | | | || ||| | || | || |||| *F Sbjct: 320 ATTAGAAATAGCTAAATCTATGTACATATTATCACA-AAATA-AATTTAAAA-AATGACT 376 *F Query: 3403 ATTTTA-AATAATTGAAATGGTAC-ATTTA-CATACATCATATTAAGTCAAATGACTTAA 3347 *F || | | |||| ||| || | ||| | | | | || | | || |||| | ||| *F Sbjct: 377 ATATAAGAATATTTGTCATTAGAGTATTGATCTTGCGTCGTGTGAAA--AAATAA-TTAG 433 *F Query: 3346 TAAATATACTCAATACAAAGCAAATTATATTAACTACCGTAATTTAAAACATAAATTTTA 3287 *F |||| | | | | || | | ||| | || | || | || | *F Sbjct: 434 GCAATA-ATTGT-TG-AGTGCTTGTG-TCGCCACTTC-GTGCCTCAAGAAATGACCAAAG 488 *F Query: 3286 CAAA-A-ATTT-AATTTTTC-ACACGCCGCAAGCTGAATACTCTAATGACAAATATTCTA 3231 *F |||| | | | ||| ||| || | | ||| | | ||| | || || || *F Sbjct: 489 CAAAGACACTAGAATAATTCTACTGTCTTTGATATGACTTTTGCAAT-A-AACCGTTATA 546 *F Query: 3230 A-TAAAGTAATTTTTGAAATTTATTTTG-TGATA-AT--ATGTA-CATAGATTTGGCTA- 3178 *F | | | ||||| ||||||||||| ||| | || || | || | || || *F Sbjct: 547 TGTTTATTTATTTTATAAATTTATTTTTATGAAATATTTATTTCTCAACGTAATGTCTTC 606 *F Query: 3177 TTTCTAATCTATTT-TCAAATAATAATAACGTTAA 3144 *F ||| || |||| | |||| | | || | ||| *F Sbjct: 607 TTTTTAGAAAATTTATGAAATTACAGTAGCTATAA 641 *F Score = 139 (26.9 bits), Expect = 7.7e-06, Sum P(2) = 7.7e-06 *F Identities = 39/50 (78%), Positives = 39/50 (78%), Strand = Minus / Plus *F Query: 3537 CAAAGACACTAGAATAACAAGATGCGCAACGGCCTTAC-ATTGGTTTGGC 3489 *F ||||||||||| ||||| ||||||| |||| || ||| ||| || | | *F Sbjct: 1 CAAAGACACTAAAATAATAAGATGCATAACG-CCATACGATTTTTTCGTC 49 # *U FBrf0137497 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG12573 on Thu Aug 16 15:08:18 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:08:18 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG12573 on Thu Aug 16 15:08:18 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG12573 *F Release: 1 *F Gene annotation error *F Gene CG12573 has incorrect exon/intron structure or translation start site. *F Comments: CG12573 shows a good match (70%id) to part of the Waldo-A transposon *F from 1-511 of its *F 1001bp. *F Query= CG12573|FBgn0040029|CT34341|FBan0012573 GO: located on: U; |cDNA *F sequence *F (1001 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 763 4.4e-31 *F 1 gb|AC005847|Waldo-B WALDOB 5180bp 237 0.00018 *F 1 gb|AJ278684|pilger DME278684 5108bp Derived from AJ278684... 235 0.00023 *F 1 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 763 (120.5 bits), Expect = 4.4e-31, P = 4.4e-31 *F Identities = 263/371 (70%), Positives = 263/371 (70%), Strand = Plus / Plus *F Query: 40 CTGCTGGTGGCGGTAGCAAAGGCTACTAACAGGGGTTCGTACCTGAAAAGAGCGCGCTCG 99 *F |||| | | | ||||| | | ||| ||||| || ||||||||| |||| || ||| *F Sbjct: 4186 CTGCACCAGTCTGGAGCAATGCC-ACTGGCAGGGTCTCATACCTGAAAGGAGCTCGTTCG 4244 *F Query: 100 GGGCTACGGTCCATGGCCCTCAGGCTCATCAGAGGTTTCAGGACCATATCCGAAGACGCG 159 *F | ||||||||| ||| | || |||||||| |||||||||||||||||||||||||||||| *F Sbjct: 4245 GTGCTACGGTCAATGTCTCTGAGGCTCATTAGAGGTTTCAGGACCATATCCGAAGACGCG 4304 *F Query: 160 GCGCTAGCACTGTCAGGCCTTCCACCAATTGATCTGGAGATCAAGGCATTCAGCCTAAAG 219 *F |||||||| ||| ||||||| || || |||||||||||||||||||| ||||||||| | *F Sbjct: 4305 GCGCTAGCGCTGGCAGGCCTGCCGCCGATTGATCTGGAGATCAAGGCTCTCAGCCTAATG 4364 *F Query: 220 CGGGGTGGCGCCTCCTGGCTAGAGGCACACGTGTGGATGCTGGCCCTAGTGGCCTGTTCG 279 *F ||| ||||||| ||| ||| ||||||||||| |||| | | | | |||| | *F Sbjct: 4365 CGGAGTGGCGCTTCCAGGCAAGAGGCACACGAGTGGCTATTAGGTGAA-TGGCA-GA--- 4419 *F Query: 280 CGGCTGGTGGCGAACAGT-GAGGCATCGGAGCTGGG-TCA-CAGCCTTATCATTAAG-TC 335 *F | | |||| ||| || | | | |||| ||| | | |||| | ||| || | *F Sbjct: 4420 \-GTA-GATGGCAAAC-GTCGCGACGGGGGAGGTGGACTTATCAGC-TCATCCCAGAGATG 4475 *F Query: 336 \-CGATCAGACGAGATGGCCAACAGAG-TGGGTGG-CTA--AGCTCAAACAGT-CGTCCCG 389 *F || | | ||| ||||||| | || ||| ||| | || | |||| | || || *F Sbjct: 4476 ACGGTTTGGGCAGAGTGCCAACACAAATGCTTGGACTACCACCTAACCCAGTTCCTCACG 4535 *F Query: 390 TGTGGTGGCGG 400 *F |||| | *F Sbjct: 4536 GACCATGGCTG 4546 *F Score = 398 (65.8 bits), Expect = 4.1e-18, Sum P(2) = 4.1e-18 *F Identities = 154/226 (68%), Positives = 154/226 (68%), Strand = Plus / Plus *F Query: 291 GAACAG-TGAGGCATCGGAGCTGG--GTCACAGCCTTATCATTAAGTCCGATCAGACGAG 347 *F || || || | || | ||| ||| ||| |||| | | | |||| |||| | *F Sbjct: 4797 GACGAGATG-GCCAACAGAG-TGGATGTCTAAGCCCAAAC-TGGTGTCCTGGGTGACG-G 4852 *F Query: 348 ATGGCCAACAGAGTGGGTGGCTAAGC-TCAAACAG-TCGTCCCGTGTGGTGGCGGGCGAA 405 *F ||| || | | | | || ||| || | | ||||| || | | | *F Sbjct: 4853 CGGGCGAAGA-ATTCA-TC-CTCAGCGTCCC-CGGCTCGTCGTAAAAGGCGACTAAAGG- 4907 *F Query: 406 GAGGAAGCAGGAGCCCTCATGGACTCCAATGAAGGATTGGAGTGCGATTTGTCCTCACAT 465 *F | ||||| |||||||| |||||||| || ||||||| ||||||||| || |||||||| *F Sbjct: 4908 GTGGAAGGAGGAGCCCCCATGGACTACACTGAAGGAAGGGAGTGCGACCTGGCCTCACAT 4967 *F Query: 466 CCTGCGCACCGAAGTCATACCTTGACTGGCAGTCCCGGGGGTCGCG 511 *F ||||| |||||||||||||||||||||||||||||||| | || | *F Sbjct: 4968 CCTGCTCACCGAAGTCATACCTTGACTGGCAGTCCCGGTGAGCGAG 5013 *F Score = 285 (48.8 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12 *F Identities = 65/75 (86%), Positives = 65/75 (86%), Strand = Plus / Plus *F Query: 333 GTCCGATCAGACGAGATGGCCAACAGAGTGGGTGGCTAAGCTCAAACAGTCGTCCCGTGT 392 *F ||||||||||||||||||||||||||||||| || |||||| ||||| | |||| | || *F Sbjct: 4788 GTCCGATCAGACGAGATGGCCAACAGAGTGGATGTCTAAGCCCAAACTGGTGTCCTGGGT 4847 *F Query: 393 GGTGGCGGGCGAAGA 407 *F | |||||||||||| *F Sbjct: 4848 GACGGCGGGCGAAGA 4862 *F Score = 280 (48.1 bits), Expect = 1.8e-06, P = 1.8e-06 *F Identities = 86/113 (76%), Positives = 86/113 (76%), Strand = Plus / Plus *F Query: 262 GCCCTAGTGGCCTGTTCGCGGCTG-G-TGGCGAACAGTGAGGCATCGGAGCTGGGTCACA 319 *F ||||||||||| ||||||||||| | |||||||||| | ||| | ||||| ||| |||| *F Sbjct: 4702 GCCCTAGTGGCTTGTTCGCGGCTATGATGGCGAACAGGGGGGCTTGGGAGCGGGGACACA 4761 *F Query: 320 GCCTTATCATTAAGTCCGATCAGACGAGATGGCCAA-CAGAGTGGGTGGCTAA 371 *F || |||||||||| | ||| | | | | || || | |||| | |||| || *F Sbjct: 4762 GCATTATCATTAA-TATGATGA-A-GCG-TGTCCGATCAGACGAGATGGCCAA 4810 *F Score = 261 (45.2 bits), Expect = 4.1e-18, Sum P(2) = 4.1e-18 *F Identities = 195/330 (59%), Positives = 195/330 (59%), Strand = Plus / Plus *F Query: 1 ATGCCCAACTTCGGTGGCCCAAGATCTCCGGCCAGGAAACTGCTGGTGGCGGTAGCAAAG 60 *F ||||||||| |||| ||||||||| ||||||||||||||||||||| | ||||||||| *F Sbjct: 4110 ATGCCCAACGTCGGAGGCCCAAGACACCCGGCCAGGAAACTGCTGGTGTCAGTAGCAAAG 4169 *F Query: 61 GCTACTA-AC-AGGGGTTCGTACCTGAAAAGAGCGC-GCTCGGGGCTACGGTC-CATGGC 116 *F ||| | || | | | | ||| | |||| || ||| | |||| ||| | *F Sbjct: 4170 GCTTCGCTACTATACGCT-GCACCAGTCTGGAGCAATGCCACTGGC-AGGGTCTCATACC 4227 *F Query: 117 CCTCAGG--CTCATCAGAGGTTTCAGGACCATATCCGAAGACGCGGCGCTAGCACTGTCA 174 *F ||| ||| | | | | | || | || || || || | ||| | ||| *F Sbjct: 4228 TGAAAGGAGCTCGTTCGGTGCTAC-GGTCAATGTCTCT-GAGGCT-CATTAGAGGTTTCA 4284 *F Query: 175 GG-CC-T-TCC-ACCAATTGAT-CTGGAGATCA-AGGCATTCAGCCTAAAGCGGGGTGGC 228 *F || || | ||| | | | || | | | |||| | | ||| | | ||| *F Sbjct: 4285 GGACCATATCCGAAGACGCGGCGCTAGCGCTGGCAGGCCTGCCGCCGATTGATC--TGGA 4342 *F Query: 229 GCCTCCTGGCTAGAGGCACACGTGTGGATGCTGGCCCTAGT-GGCCTGTTCGCGGCTG-G 286 *F | || |||| || | || ||| | |||| || ||| | || | | *F Sbjct: 4343 GA-TCAAGGCTCTCAGCCTAA-TGCGGA-G-TGGCGCTTCCAGGCAAGAG-GCACACGAG 4397 *F Query: 287 TGGCGAACAG-TGA--GGCATCGGAGCTGG 313 *F |||| | || ||| |||| | || ||| *F Sbjct: 4398 TGGCTATTAGGTGAATGGCAGAGTAGATGG 4427 # *U FBrf0137498 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG12614 on Thu Aug 16 15:11:53 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:11:53 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG12614 on Thu Aug 16 15:11:53 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG12614 *F Release: 1 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG12614. *F Comments: CG12614 can be deleted from GadFly--it appears to be part of a *F Waldo-A element (just 85% identity *F but over its whole length). *F Query= CG12614|FBgn0039981|CT35018|FBan0012614 GO: located on: U; |cDNA *F sequence *F (318 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 1170 1.5e-49 1 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 1170 (181.6 bits), Expect = 1.5e-49, P = 1.5e-49 *F Identities = 270/315 (85%), Positives = 270/315 (85%), Strand = Plus / Plus *F Query: 1 ATGAATGGCAAGATAAACATCCTGCTATCCGCATTCGAGACTCAACGGAACGTCACAAGA 60 *F |||||||||||||| | || | ||||||||||||||||||| |||||| |||| ||| || *F Sbjct: 752 ATGAATGGCAAGATCAGCACCTTGCTATCCGCATTCGAGACCCAACGGCACGTGACACGA 811 *F Query: 61 GAGACTAAGGGCGTAGCTTCTGAACGCTCAGCCCTTAACAACAGGGCGAAAGAGCTGTAT 120 *F ||||| |||||| ||||||| |||| |||||||||||||||||||||| ||||||||| *F Sbjct: 812 GAGACAAAGGGCATAGCTTCCGAACTCTCAGCCCTTAACAACAGGGCGTTAGAGCTGTAC 871 *F Query: 121 GAAGGTGCAACTAGCGAGCACCGCGTGACGACCAGTTCTACCCAGACGGAGCCAAAAAAG 180 *F ||||| | ||||||||||||||| ||| || |||| |||||||||||||| | ||||| *F Sbjct: 872 AAAGGTACTACTAGCGAGCACCGCTTGAAGAGCAGTGCTACCCAGACGGAGGTACAAAAG 931 *F Query: 181 CCAACCAAAAAGGTTTCTCACAATGCGCCGGAGCCCAAGGGGCAAGTGCCCAAAGTCCAG 240 *F || |||||||||| | |||| ||||| | || ||||||| ||||||||| ||| ||||| *F Sbjct: 932 CCTACCAAAAAGGCTCCTCAAAATGCACAGGTACCCAAGGTGCAAGTGCCAAAAATCCAG 991 *F Query: 241 GCGCCCAAGCCGAGGAACAACACGATGGGTGGCAACGGTGAAGGAAAGGCGACACCCAGA 300 *F |||||||||||||| ||| ||| |||||||||||||||||| |||||| ||| ||||| *F Sbjct: 992 GCGCCCAAGCCGAGCAACCCTACGTTGGGTGGCAACGGTGAAGAAAAGGCAACATCCAGA 1051 *F Query: 301 CCAAATGAGTCCAAA 315 *F ||||| ||||||||| *F Sbjct: 1052 CCAAACGAGTCCAAA 1066 # *U FBrf0137499 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG17439 on Thu Aug 16 15:15:33 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:15:33 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG17439 on Thu Aug 16 15:15:33 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG17439 *F Release: 1 *F Gene annotation error *F Gene CG17439 has a mistake in the supporting evidence or functional assignment. *F Comments: CG17439 can be deleted from GadFly--it appears to be part of an X *F element (67% identity but over whole length of gene). *F Query= CG17439|FBgn0037210|CT38533|FBan0017439 GO: located on: 3L *F 80B2-80B2; |cDNA sequence *F (616 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 720 1.1e-39 *F 2 gb|X06950|G-element DMREPG 4346bp Derived from X06950 (g8... 130 0.9998 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 720 (114.1 bits), Expect = 1.1e-39, Sum P(2) = 1.1e-39 *F Identities = 260/387 (67%), Positives = 260/387 (67%), Strand = Plus / Plus *F Query: 236 GACGTAGGCCT-ACCCGCGACGTCAATGTTG-GC-CACATTTCATGCTACTGACGTCCAG 292 *F ||||| || | ||| | | ||| | || | ||| || | | || ||||| *F Sbjct: 3855 GACGTGTGCGTCACCTAC-AGGTCCC-GATGCGAGCACGACGCAGCCGA-TGGTATCCAG 3911 *F Query: 293 GACTTCTCATTAACATTTG-CGGAATGGGCAAAACGCTGGAACATTGGCATCAATGGTGA 351 *F ||||| ||| | | || |||||||||||| ||| ||||| |||||||||||| | *F Sbjct: 3912 GACTTTGCAT-ACCGGTTCTCGGAATGGGCAAGACGATGGAATATTGGCATCAATAGCAG 3970 *F Query: 352 CAAATCTGCGAATGTGTGCTACACGCTGAAAAGGAAAACATCACCGGCTTTACT-CATCG 410 *F ||||| || || |||| ||| | || ||| ||| ||||| | | || ||||| *F Sbjct: 3971 TAAATCCAACAACGTCTGCTTCACTTTAAAGCGGAGAACGCCACCGCCCGT-CTACATCG 4029 *F Query: 411 ATGGC-TCCCTTATCCCACAGTCCAGTTCAGCCAAATATCTTGGTGTAATATTGGATCGG 469 *F | | |||| | |||||| | | |||| || || ||||| || | ||||||| *F Sbjct: 4030 AGGAAGTCCCCG-TACCACAGCCGAACGCAGCAAAGTACCTTGGAGTGCTTCTGGATCGC 4088 *F Query: 470 AGACTAAACTTCTCGAAGCAAAATAGTGCGATAAGAGTGCGTATACGTGCAGCAGCCTCA 529 *F ||||| | || || ||||| | | || ||| ||| ||||||| | | *F Sbjct: 4089 AGACTCACATTTTCCAAGCATGTGACCGACATCAGAACGCGCCTACGTGCTAAGGTGGCG 4148 *F Query: 530 AAGCACTTCTGGCTAATTAATTCGCGAAGTAAAGTGTCACTCTCCAACAAGGTGACAATT 589 *F ||||||| ||||||| || |||||| |||||| |||| || ||||||||| |||||||| *F Sbjct: 4149 AAGCACTACTGGCTACTTTCTTCGCGCAGTAAATTGTCGCTATCCAACAAGCTGACAATT 4208 *F Query: 590 TAGAAGCAAATTATAGCGCCAATATGG 616 *F || || || || |||| |||| ||| *F Sbjct: 4209 TACAAACAGATCCTAGCACCAAACTGG 4235 *F Score = 490 (79.6 bits), Expect = 2.5e-29, Sum P(2) = 2.5e-29 *F Identities = 194/293 (66%), Positives = 194/293 (66%), Strand = Plus / Plus *F Query: 95 TCGTATCTCGAAAAGAGAAGATTTGCGGTACGATTCCATCCGGCCCTGTCAAATGAGCAT 154 *F ||||| || ||| || || || || || || || ||| | || | || | ||||| *F Sbjct: 3669 TCGTACCTGGAAGGAAGGAGGTTCGCTGTGCGCTTTCATTCAGCAATTTCCACCGAGCAC 3728 *F Query: 155 AATGTGGCAGCCGGAGTACCACA-GGTAAGTGAACTTGGCCCGCTGCTTTACTGCCTGTA 213 *F || |||||||| || || ||||| |||| ||| || ||||| ||||| ||||||||||| *F Sbjct: 3729 AACGTGGCAGCTGGTGTTCCACAAGGTA-GTGTCCTCGGCCCCCTGCTCTACTGCCTGTA 3787 *F Query: 214 CAGCTACGACATGCCTCGGCCAGACGTAGGCCTACCCGCGACGTCAATGTTGGCCACATT 273 *F ||| |||||||||| | |||||| ||| |||| || || || |||||||||||||| *F Sbjct: 3788 TAGCCACGACATGCCGCAGCCAGATGTAAGCCTTTACGGGAAATCTATGTTGGCCACATT 3847 *F Query: 274 TCATGCTACTGACGTCCAGGACTTCT-CATTAACATTTGCG-GAATGG-GCAAAACGCTG 330 *F | | | |||||| | | || || | |||| | | | ||| || || *F Sbjct: 3848 TGCCG--A-TGACGTGTGCGTCACCTACAGGTCCCGATGCGAGCACGACGCAGC-CGATG 3903 *F Query: 331 GAACATTGGCATCAATGGTGACAAAT-CTGCGAATGTGTGCTACACGCTGAAA 382 *F | | || | | || || | || ||||| | || | ||| || || *F Sbjct: 3904 GTATCCAGG-A-CTTTGCATACCGGTTCTCGGAATG-G-GCAAGACGATGGAA 3952 *F Score = 309 (52.4 bits), Expect = 1.1e-39, Sum P(2) = 1.1e-39 *F Identities = 81/105 (77%), Positives = 81/105 (77%), Strand = Plus / Plus *F Query: 1 TAAAGACACTACCGAGATGTGTGATCCTCTACATTACGTTGTTATTCAAGGCTATTGTGA 60 *F | ||| | || || ||| | |||||||||||| |||||| | ||||| |||||||||| *F Sbjct: 3217 TGAAGGCCCTGCCCAGACAAGCGATCCTCTACATAACGTTGGTTTTCAACGCTATTGTGA 3276 *F Query: 61 GACTGCAATACTTCCTTCCACAGTGGAAGCTCGGTCGTATCTCGA 105 *F | |||||||||||| | |||||||||||||| ||||| | *F Sbjct: 3277 GGTTGCAATACTTCCCTTATCAGTGGAAGCTCGGGATAATCTCCA 3321 # *U FBrf0137500 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG18225 on Thu Aug 16 15:18:25 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:18:25 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG18225 on Thu Aug 16 15:18:25 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG18225 *F Release: 1 *F Gene annotation error *F Gene CG18225 has a mistake in the supporting evidence or functional assignment. *F Comments: CG18225 can be deleted--it appears to be part of a Waldo-A element *F (just 83% identity *F but over its whole length). *F Query= CG18225|FBgn0033011|CT41254|FBan0018225 GO: located on: 2R *F 41F3-41F3; |cDNA sequence *F (306 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AC005734|Waldo-A WALDOA 5150bp 963 3.7e-40 1 *F >gb|AC005734|Waldo-A WALDOA 5150bp *F Length = 5171 *F Plus Strand HSPs: *F Score = 963 (150.5 bits), Expect = 3.7e-40, P = 3.7e-40 *F Identities = 231/276 (83%), Positives = 231/276 (83%), Strand = Plus / Plus *F Query: 1 ATGGCCCTTAATCCTCGGAGCCATGAACTGAATGGGGATTGCGGAGTATCTGAGGATAGT 60 *F ||||||| ||||||||| |||||| || | |||||||| ||||||| || |||||||| *F Sbjct: 3554 ATGGCCCGTAATCCTCGCGGCCATGTACCGTATGGGGATCCCGGAGTACCTAAGGATAGT 3613 *F Query: 61 CGTTGGCAGCTACTTTACGGACCGGGTCCTATGGTGCGATACGGAAGCTGGCCCAAAATG 120 *F ||||||||||||||||| ||||||||||||||||| ||||||||||| |||||||||| | *F Sbjct: 3614 CGTTGGCAGCTACTTTAGGGACCGGGTCCTATGGTACGATACGGAAGATGGCCCAAAAAG 3673 *F Query: 121 ATACCGAGTTTCGGCAGGTGTTCCCCAAGAATCGGTACGTGGACCAATCCTGTAGAACAT 180 *F ||||||||||||||||||||||||||||| |||||||| |||||||||||| | |||||| *F Sbjct: 3674 ATACCGAGTTTCGGCAGGTGTTCCCCAAGGATCGGTACTTGGACCAATCCTATGGAACAT 3733 *F Query: 181 CATGTACGATGGGATCTTGGGCATCAACAGGCCCGTGGGAGTAGGGTGGCAAT-TACAGC 239 *F ||||||||||||||||||||||||||||||||||| ||||||| | ||| | | || *F Sbjct: 3734 TATGTACGATGGGATCTTGGGCATCAACAGGCCCGTAGGAGTAGAGCTGCATTGTTTTGC 3793 *F Query: 240 AGCCGCCAAAACAATCGCAGG-GTTGCAAGATAAAT 274 *F | || ||||| ||| || | | | ||| *F Sbjct: 3794 TGACGATGTGGCAATCACAGCTGTCTCGAAAACAAT 3829 *F Score = 284 (48.7 bits), Expect = 2.3e-09, P = 2.3e-09 *F Identities = 132/211 (62%), Positives = 132/211 (62%), Strand = Plus / Plus *F Query: 96 GCGATACGGAAGCTGGCCCAAAATGATACCGAGTTTCGGCAGGTGTTCCCCAAGAATCGG 155 *F | | | ||| || || || || ||| | | || | | | | || | | | *F Sbjct: 3679 GAGTTTCGGCAGGTGTTCCCCAAGGAT-CGGTACTTGGACCAATCCTATGGAACATTATG 3737 *F Query: 156 TACG-TGGACCAATCCTGTAGAACATCATGTACGATGGGATCTTGGGCATCAACAGGCCC 214 *F |||| ||| ||| || | || || | || | ||| | | || || | *F Sbjct: 3738 TACGATGGG---ATCTTGGGCATCAACAGGCCCG-TAGGAG-TAGAGCTGCATT-GTTTT 3791 *F Query: 215 GTGGGAGTAGGGTGGCAATTACAGCAGCCGCCAAAACAATCGCAGGGTTGCAAGATAAAT 274 *F | | | | | ||||||| ||||| | | | |||||||||||||||||| |||| |||| *F Sbjct: 3792 GCTGACG-ATG-TGGCAATCACAGCTGTCTCGAAAACAATCGCAGGGTTGGAAGACAAAT 3849 *F Query: 275 GTAACATTACGATTGGTGCTACCATCCACTG 305 *F | ||| |||||| |||||| |||||| ||| *F Sbjct: 3850 GCAACTCTACGATCGGTGCTGCCATCCGCTG 3880 # *U FBrf0137501 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG2042 on Thu Aug 16 15:31:11 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:31:11 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG2042 on Thu Aug 16 15:31:11 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG2042 *F Release: 1 *F Gene annotation error *F Gene CG2042 has incorrect exon/intron structure or translation start site. *F Comments: CG2042 shows a good match (71%) to the X element from 166-662 of its *F 774bp. *F Query= CG2042|FBgn0032944|CT3379|FBan0002042 GO: located on: 2L 39D3-39D3; *F |cDNA sequence *F (774 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 876 1.1e-48 *F 2 gb|X77571|BS DMBSTE 5126bp Derived from X77571 (g453966) ... 189 0.020 *F 1 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 876 (137.5 bits), Expect = 1.1e-48, Sum P(2) = 1.1e-48 *F Identities = 264/369 (71%), Positives = 264/369 (71%), Strand = Plus / Plus *F Query: 296 AAGAAGCTGTCGACACT-CTCACCCATAATATCAAGGCTGCAGCCAGACTGGCAAGACCT 354 *F | || ||| |||| ||| |||||| | ||||||||| | ||||| ||| |||||| | *F Sbjct: 2617 ATGATGCTATCGA-ACTGCTCACCAACAATATCAAGTCAGCAGCTAGATTGGCAACTCGC 2675 *F Query: 355 AGCCCGGTGCAGAGGCCCACAGCAGCTAAAATCCCGATCACCAGAGAGATACTGCTCCTT 414 *F ||| | | |||| | |||| | |||||| || |||| ||||| ||||| ||| *F Sbjct: 2676 AGCATATCTCGGCAGCCCGCGGCAGATCGAATCCCAATACCCAGGGAGATCCTGCTGCTT 2735 *F Query: 415 ATAGCTGAGAGGAGGTGTTTACGCAAAAAAAGGATGAGGTCTCGCCACCCGCTGAACAAA 474 *F |||||||||| |||| | ||||||| | ||||||||||||| |||||| | ||||| *F Sbjct: 2736 ATAGCTGAGAAGAGGCGCTTACGCACTAGGTGGATGAGGTCTCGGCACCCGTCGGACAAA 2795 *F Query: 475 GCGGATTGGAACAGAGCAAAGTGTGTTTTAC-ATCGCGCCCTGGTCGAACATAAATCAGC 533 *F |||| |||||| |||| | || | | || |||| |||| || ||| | || *F Sbjct: 2796 ACGGAATGGAACCGAGCTCTGAGTAGGCTCCGAT-GCGCGTTGGTGCTGCACAAAGCCGC 2854 *F Query: 534 TTGGTTCTATGACAGGTTTGCAAACATTGGAAACGAATGTGATGCGACGCACTCGCTATG 593 *F |||||| | || ||| |||| || | ||| |||| | || |||||||| ||||| || *F Sbjct: 2855 ATGGTTCGACGAAAGGCTTGCCAATACCGGAGTCGAAAGCGAAGCGACGCATTCGCTGTG 2914 *F Query: 594 GAATGCTACGCGCGCTATCCAAAAGCATTGTGCAAGGAAAGCGCCATTGGCTGATAATAA 653 *F ||| || |||||||| ||| ||| || ||| | ||||| ||||| | | |||| || *F Sbjct: 2915 GAAGGCCACGCGCGCAATCAAAAGGCGTTGCACGAGGAAGGCGCCTCTAGTCGATAGCAA 2974 *F Query: 654 CGGTGCATG 662 *F ||| |||| *F Sbjct: 2975 CGGGACATG 2983 *F Score = 353 (59.0 bits), Expect = 1.1e-48, Sum P(2) = 1.1e-48 *F Identities = 107/151 (70%), Positives = 107/151 (70%), Strand = Plus / Plus *F Query: 166 GCGGGCGACTGGAATACATCCCACTGGCTTTGGGGAGCGGAGAGATGCAATCGAAGAGGG 225 *F || ||||| ||||| | ||||||||||| ||||| |||| || |||| | |||||| *F Sbjct: 2229 GCAGGCGATTGGAACGCGTCCCACTGGCTCTGGGGTGCGGGAAGGAGCAACCAAAGAGGC 2288 *F Query: 226 CGTGCGCTAGCGAATCTGGTCCTAAGCACAGGTATGAGCTCCCTAGTAACCGGAGGTCCT 285 *F ||| |||||||||| ||||||| | | || ||| |||| ||| ||||| || *F Sbjct: 2289 ATTGCATTAGCGAATCTCGTCCTAAATTCGGAGGTGGACTCGCTAGCAACAGGAGGACCA 2348 *F Query: 286 AAAAGATTCCAAGAAG-CTGTCGACACTCTC 315 *F | ||||| || | | |||| || ||| | *F Sbjct: 2349 ACAAGATACCCGTACGGCTGTAGAGGCTCAC 2379 # *U FBrf0137502 *a Misra *b S. *t 2001.8.16 *T personal communication to FlyBase *u FlyBase error report for CG5929 on Thu Aug 16 15:34:11 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 Aug 2001 15:34:11 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG5929 on Thu Aug 16 15:34:11 2001 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG5929 *F Release: 1 *F Gene annotation error *F Gene CG5929 has incorrect exon/intron structure or translation start site. *F Comments: CG5929 shows a good match (76%) to the X element from 1287-1664 of its *F 2084bp. *F Query= CG5929|FBgn0032982|CT18625|FBan0005929 GO: located on: 2L *F 40C3-40D1; |cDNA sequence *F (2084 letters) *F Database: /data/blast/db/na_te.dros *F 55 sequences; 282,078 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High *F Probability Sequences producing High-scoring Segment Pairs: Score *F P(N) N *F gb|AF237761|X-element ROXELEMENT 4740bp 603 1.4e-35 2 *F >gb|AF237761|X-element ROXELEMENT 4740bp *F Length = 4740 *F Plus Strand HSPs: *F Score = 603 (96.5 bits), Expect = 1.4e-35, Sum P(2) = 1.4e-35 *F Identities = 163/213 (76%), Positives = 163/213 (76%), Strand = Plus / Plus *F Query: 1287 TTTGTGCGGCGCTACGAGATCGATATTCTGCTGCTCAGCGAGACTCATTGCAAGGAGGCA 1346 *F || ||||| || |||| |||||| | | |||||||||||||| || ||||||| |||| *F Sbjct: 1896 TTCGTGCGACGTCACGAAATCGATGTATTACTGCTCAGCGAGACACACTGCAAGGGGGCA 1955 *F Query: 1347 CAGGCACCCAAAATATTTGGATTTG-AGTCTTACACTGCCAACGACCTGAGCGACGGTAA 1405 *F || | || || |||| ||||||| || || |||||||||| || | ||| | || || *F Sbjct: 1956 GAGACGCCTAAGCTATTCGGATTTGTAGCCT-ACACTGCCAATGATCCGAGTGGTGGCAA 2014 *F Query: 1406 CGCCAAAGGTGTTGCAGCGATCCTGGTCAAATCCGGCCTAGTCCACTTTCCTCTTACACC 1465 *F ||||||||| | ||||| ||| | ||||| |||| | ||||||||| || ||||| *F Sbjct: 2015 CGCCAAAGGCGGAGCAGCTATCTTAATCAAAAATAGCCTTGCCCACTTTCCGCTAACACC 2074 *F Query: 1466 AATAGCCACTGACGAGGAGCAACTCGCGTCTGC 1498 *F ||||||||||| | ||| |||||| ||| | || *F Sbjct: 2075 AATAGCCACTGCCAAGGTGCAACTTGCGCCGGC 2107 *F Score = 469 (76.4 bits), Expect = 1.4e-35, Sum P(2) = 1.4e-35 *F Identities = 145/203 (71%), Positives = 145/203 (71%), Strand = Plus / Plus *F Query: 1463 ACCAAT-AGCCACTGACGAGGAGCAACTCGCGTCTGCCCTACGAGATATAAGCTCCCTAG 1521 *F ||||| || || || | | ||| | || |||| | || || | ||| |||| *F Sbjct: 2278 ACCAAAGAGGCATTG-C-ATTAGCGAATCTCGTCCTAAATTCG-GAGGTGGACTCGCTAG 2334 *F Query: 1522 CCACCGGAGGTCCTACAAGATTCCCGTTCGGCAGTAGAGGCTCCCCAGGCTATATAGATT 1581 *F | || ||||| || ||||||| ||||| |||| |||||||||| ||||| || || |||| *F Sbjct: 2335 CAACAGGAGGACCAACAAGATACCCGTACGGCTGTAGAGGCTCACCAGGGTACATCGATT 2394 *F Query: 1582 TTGCTGTCAGCAAGGGCATTCTGGATATGCACGATGAAATACGTTCCGTTGCTGAGCTCA 1641 *F |||| | | ||||| | |||| || |||| | | ||| || | |||| |||||| | *F Sbjct: 2395 TTGCACTGACAAAGGGTGTGCTGGGCATCCACGCTAACATAAGTGCGGTTGTTGAGCTTA 2454 *F Query: 1642 GCTCCGACCACCTACCGCTTATA 1664 *F ||||||||||||| || || || *F Sbjct: 2455 GCTCCGACCACCTGCCTCTGGTA 2477 # *U FBrf0137503 *a Salz *b H. *t 2001.7.18 *T personal communication to FlyBase *u *F From hks@po.cwru.edu Wed Jul 18 17:19:42 2001 *F To: Rachel Drysdale (Genetics) *F Subject: naming snRNP proteins *F Cc: smount@wam.umd.edu, sima@fruitfly.bdgp.berkeley.edu, ma11@gen.cam.ac.uk *F Rachel, here is the official notification that we have shown that *F l(2)43Ee published in Heitzler et al. 1993, Genetics 135:105.) is U2A *F (FBgn0033210), the only allele of the gene is now called U2A1. *F Other information that flybase might want to capture is that l(2)43Ed is an *F allele of Rpt1, as are l(2)05643 and EP(2)2153. *F Helen Salz Ph.D. *F Department of Genetics, BRB 626 *F Case Western Reserve University *F 10900 Euclid Avenue *F Cleveland, Ohio 44106-4955 *F Telephone: (216)368-2879 *F FAX: (216)368-3432 *F Email: hks@po.cwru.edu *F http://www.RNAresearch.org *F http://genetics.cwru.edu # *U FBrf0137504 *a Laurencon *b A. *t 2001.7.21 *T personal communication to FlyBase *u *F From Laurencon@maccgmc.univ-lyon1.fr Sat Jul 21 01:08:17 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: mus108 *F Dear flybase persons, please post this set of data for mus108. *F Please find the following informations about mus108 mutation mapping, *F that I am not thinking about publishing in journals since I am not *F pursuing this mapping further. *F The following Df delete/disrupt mus108: *F Df(1)JC70 *F Df(1)A113 *F Df(1)ovoG6 *F Df(1)ovoG7 *F and two Df does not delete/disrupt mus108: *F Df(1)dhd81 *F Df(1)RC40 *F I join to this e-mail a table with the relevant numbers. Treatments *F as described in Sibon et al., 1999. *F As a consequence this work maps mus108 in 4E1-4EF2. The gene is *F located between the right breakpoint of Df(1)RC40 and the right *F breakpoint of one of the following deficiencies [Df(1)A113, *F Df(1)ovoG6, Df(1)ovoG7]. *F Sincerely yours, *F Anne Laurencon *F ____________________________________________________________ *F | | | *F | MMS-0.08% | MMS-0.1% | Control *F crosses |108 sibs | 108 sibs| 108 sibs *F __________________________|___________|__________|__________ *F mus108 /FM7c \* mus108 | 172 1002 | 153 661 | 519 919 *F | | | *F C(1)DX, y w f \* w mus108 | | 54 637 | 109 329 *F __________________________|___________|__________|__________ *F |108/Df FM7 |108/Df FM7|108/Df FM7 *F __________________________|___________|__________|__________ *F Df(1)JC70/FM7 \* mus108 | 350 644 | 93 330 | 113 145 *F | | | *F Df(1)A113/FM7 \* mus108 | 3 45 | 0 22 | 68 140 *F | | | *F Df(1)ovo6/FM7* mus108 | | 15 141 | 265 635 *F | | | *F Df(1)ovo7/FM7 \* mus108 | | 68 451 | 265 647 *F | | | *F Df(1)dhd81/FM7* mus108 | | 167 178 | 143 157 *F | | | *F Df(1)RC40/FM7 \* mus108 | | 46 152 | 118 338 *F __________________________|___________|__________|__________ # *U FBrf0137505 *a Laurencon *b A. *t 2001.7.23 *T personal communication to FlyBase *u *F From Laurencon@maccgmc.univ-lyon1.fr Mon Jul 23 22:36:31 2001 *F To: rd120@gen.cam.ac.uk *F Subject: more data *F Dear Rachel, *F This work has been done with mus106D1, *F The following Df delete/disrupt mus106: *F Df(1)g-1 *F and we tested two other Df that does not delete/disrupt mus106: *F Df(1)N105 *F Df(1)C246 *F I join to this e-mail a table with the relevant numbers. Treatments *F as described in Sibon et al., 1999. *F As a consequence this work maps mus106 in 12A3-10:12B7, since it has *F been previously mapped by recombination to the left of g. The gene is *F thus located between up and g. *F We have been testing different P elements for complementation, and *F they all complement the MMS sensitivity. *F EP(X)0385 *F EP(X)1396 *F EP(X)0395 *F EP(X)1329 *F EP(X)1353 *F We have also made sure that mus101 that is in the same interval is *F not allelic to mus106. *F Sincerely yours, *F Anne Laurencon *F | | | *F | MMS-0.08% | MMS-0.1% | Control *F crosses |106 sibs | 106 sibs | 106 sibs *F __________________________|___________|___________|__________ *F w mus106/FM7c \* w mus106 | 9 24 | 13 26 | 120 64 *F | | | *F |106/Df sibs|106/Df sibs|106/Df sibs *F __________________________|___________|___________|__________ *F Df(1)N105/FM6 \* w mus106 | 33 66 | 22 37 | 69 150 *F | | | *F Df(1)C246/FM6 \* w mus106 | 433 82 | 38 33 | 53 88 *F | | | *F Df(1)g-1, f B/In(1)AM | | | *F \* w mus106 | 0 37 | 0 63 | 94 152 *F __________________________|___________|___________|__________ *F |106/EP sibs|106/EP sibs|106/EP sibs *F __________________________|___________|___________|__________ *F w mus106/FM7c \* w EP(X)1396| 25 17 | 25 5 | 91 187 *F | | | *F w mus106/FM7c \* w EP(X)1353| 13 7 | 9 3 | 79 164 *F | | | *F w mus106/FM7c \* w EP(X)1329| 74 48 | 26 3 | 91 191 *F | | | *F w mus106/FM7c \* w EP(X)0395| 90 86 | 42 8 | 67 136 *F | | | *F w mus106/FM7c \* w EP(X)0385| 33 23 | 27 8 | 74 183 *F __________________________|___________|___________|__________ *F |106/101sibs|106/101sibs|106/101sibs *F __________________________|___________|___________|__________ *F w mus106D1/FM7c | 18 13 | 35 20 | 75 165 *F \* w mus101D2 | | | *F __________________________|___________|___________|__________ # *U FBrf0137506 *a Cook *b K. *t 2001.7.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 24 23:08:49 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Revised Df(3L)delta1AK breakpoints *F I reanalyzed the cytological breakpoints of Df(3L)delta1AK (FBab0024860) in *F polytene squashes and determined them to be 79E5-F1;79F2-6. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137507 *a Roote *b J. *t 2001.7.30 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Mon Jul 30 16:13:57 2001 *F To: rd120@mole.bio.cam.ac.uk *F Subject: 35De = 35Dh? *F Dear Rachel, *F I'd like to flag up the possibility that 35De = 35Dh. We only have one *F allele of each and 35DeAM2/35DhAS64 transheterozygotes have the *F heldout wing phenotype typical of the common escapers of AM2/deletion *F and AS64/deletion. *F Also we have no aberrations which separate them. *F J *F From rd120@gen.cam.ac.uk Tue Jul 31 14:17:17 2001 *F To: j.roote@gen.cam.ac.uk *F Subject: Re: 35De = 35Dh? *F Hi John, *F I'm trying to decide whether we should merge the gene records for 35De *F and 35Dh. *F According to data from *F \*E FBrf0135740 == Cai et al., 2001, EMBO J. 20(7): 1704--1714 *F 35De and 35Dh both lie between Gli and 35Ea (though it is only a *F summary diagram). *F According to the genome sequencing project (Geneseen), there is 3.5kb *F between Gli and 35Ea, most of which is taken up by BG:DS09217.4. (We *F have a note that l(2)35Dh may correspond to BG:DS09217.4, but no data *F never mind proof). so it seems reasonable to suppose that *F BG:DS09217.4, 35De and 35Dh might all collapse down to one thing in the end. *F Your data seem to say that 35DeAM2 fails to complement 35DhAS64. *F If you agree that this is what you want to say then we would, on the *F basis of that data, merge the two gene records. However I notice that you *F stop short of asking me to merge them. Natural caution? The two mutant *F chromosomes seem to have come from different pasts so it doesn't seem *F likely that they would share second site stuff does it? *F Rachel. *F From j.roote@gen.cam.ac.uk Tue Jul 31 14:53:39 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: 35De = 35Dh? *F >so it seems reasonable to suppose that *F >BG:DS09217.4, 35De and 35Dh might all collapse down to one thing in the end. *F that's my guess. *F >Your data seem to say that 35DeAM2 fails to complement 35DhAS64. *F >If you agree that this is what you want to say then we would, on the *F >basis of that data, merge the two gene records. *F good idea \- let's be brave, DO IT! *F J # *U FBrf0137508 *a Garcia-Bellido *b A. *t 1997.1.20 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Jul 31 00:39:54 2001 *F Subject: Insertions of P{f+13} *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Antonio Garcia-Bellido, Universidad Autonoma de *F Madrid *F To: Bloomington Drosophila Stock Center *F Subject: Insertions of P{f+13} *F Dated: 20 January 1997 *F Information communicated: *F Insertion Symbol Insertion Site *F P{f+13}30B 30B *F P{f+13}37C 37C *F P{f+13}44C 44C *F P{f+13}47B 47B *F P{f+13}77A 77A *F P{f+13}77C 77C *F P{f+13}87D 87D *F P{f+13}98B 98B # *U FBrf0137509 *a Umea Stock Center *b ?. *t 2001.7.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jul 30 19:05:14 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: l(3)hem2 allele *F Mime-Version: 1.0 *F The following information was given by the Umea Stock Center stock *F list. The l(3)hem2 allele was isolated by Elisabeth Gateff. It *F originated during the transposition of a P{lacW} element, but there is no P *F inserted at the l(3)hem locus (presumably a 'hit-and-run' event). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137510 *a Ellis *b M. *t 2001.5.4 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Jul 31 00:33:44 2001 *F Subject: Insertions in SNP stocks *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Mike Ellis, Exelixis *F To: Bloomington Drosophila Stock Center *F Subject: Insertions in SNP stocks *F Dated: 4 May 2001 *F Information communicated: *F SNP lines referred to in Hoskins et al., 2001, FBrf0136956, by *F Q number designations carry P insertions as listed below. *F Q 1028: P{CaSpeR}cx134.16 (3D,E) *F Q 1030: P{CaSpeR}cx131.6 (10E) *F Q 1034: P{CaSpeR}cx35.1 (6E) *F Q 1035: P{CaSpeR}SH8.1a P{CaSpeR}SH8.1b (13E9-F1, 17C) *F Q 1037: P{lacW}M64 (22D1,2) *F Q 1040: P{lacW}G38 (29C1,2) *F Q 1042: P{lacW}J29 (35E1,2) *F Q 1047: P{lwB}Z90 (57A3-6) *F Q 1049: P{lacW}K61 (49D1-3) *F Q 1050: P{lacW}D159 (62B4-5) *F Q 1052: P{lacW}J45 (68A1,2) *F Q 1056: P{lwB}AR50 (83A7-8) *F Q 1058: P{lwB}K33 (96F10,11) *F Q 1059: P{lacW}B172 (91A1,2) *F Q 1060: P{lacW}G106 (73A1-4) *F Q 1805: P{EP}EP813 *F The stock described in that paper as w;iso2;iso3 carries the *F allele w1118. # *U FBrf0137511 *a Ito *b N. *t 2001.7.31 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 31 22:14:20 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{arm-lacZ.V}70C insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Naoto Ito, Harvard University (7/01). *F P{arm-lacZ.V}70C is a homozygous viable and fertile insertion at 70C. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137512 *a Treisman *b J. *t 2001.7.31 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 31 22:21:44 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: dacE462 *F The following information accompanied a stock donated the the Bloomington *F Stock Center by Jessica Treisman (6/01). *F dacE462 is a recessive allele of dac. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137513 *a Forte *b M. *t 2001.7.31 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 31 22:25:57 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: G-salpha60AR60c *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Mike Forte, Oregon Health Sciences University (7/01). *F G-salpha60AR60c is a null allele of the G-salpha60A (=dgs) locus. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137514 *a Deal *b M. *c J. *d Deal *e K. *f Cook *t 2001.8.1 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Aug 01 15:10:49 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2L)BSC6 *F Isolation and Characterization of Df(2L)BSC6 *F Megan Deal, Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Df(2L)BSC6 was isolated as a P transposase-induced male recombination event *F involving P{lacW}l(2)k09923k09923 and P{PZ}cup01355. The deletion was *F isolated as a dpov1-cn1 recombinant chromosome from the cross dpov1 *F cn1 bw1 females x dpov1 *F P{w+mC=lacW}l(2)k09923k09923/P{ry+t7.2=PZ}cup01355 cn1; TMS, *F delta2-3/+ males. The miniwhite marker in the P{lacW} construct is *F disrupted or deleted in the Df(2L)BSC6 chromosome based on w- eye color *F in a w1 background. Polytene chromosome squashes showed the breakpoints *F 26D3-E1;26F4-7. Df(2L)BSC6 failed to complement cli1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137515 *a Rorth *b P. *c C. *d Roos *t 2001.8.16 *T personal communication to FlyBase *u *F From pernille.rorth@embl-heidelberg.de Wed Aug 08 17:33:17 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: CG8222/vgr1 and PDGF *F Dear Flybase, *F My lab is in the process of publishing a paper on the function of a *F receptor tyrosine kinase which is called 'vgr1' in Flybase and its ligand *F CG7103, a PDGF-related molecule which is annotated in a confusion way due *F to confusing Genbank submissions. *F We initially called the receptor DPR (for Drosophila PDGF Receptor related *F protein) and the ligand DPL1 (for Drosophila PDGF Like-1 \- there are two *F other putative ligands in the genome which we call DPL2 and DPL3). There *F are several reasons why I think the name vgr1 should not be used for the *F receptor (see below). I would prefer if we could agree on naming of *F receptor and ligand before publication and am therefore writing to you now. *F The receptor: *F By simple blast search with the 'VGR1' or CG8222 protein sequence, the *F mammalian PDGF-alpha receptor is the most closely related (e-73 versus e-53 *F for the most closely related VEGF Receptor). The number of extra-cellular *F Ig domains is more similar to VEGF receptor than to PDGF receptor. Thus *F the receptor is part of the PDGFR/VEGFR (super-)family but not orthologue *F of a particular receptor (same is true for the ligands, see below). Since *F PDGFR is better known than VEGFR and the ligands are identified by having a *F PDGF domain, a name reflecting this is appropriate. *F There is only one receptor in the fly genome corresponding to the whole *F family of mammalian PDGF receptors and VEGF receptors, thus calling it VGR1 *F is misleading. *F The Vgr1 sequence is only submitted to Genbank, no manuscript or abstract *F cited. We have data for both function and biochemical interaction with the *F ligand. *F Maybe DPR is not optimal \- perhaps we can compromise on PVR (for PDGF- and *F VEGF- Receptor related)? *F The ligands: *F They are defined by having a PDGF domain. We have biochemical data that *F what we call DPL1 (CG7103) binds to and activates DPR. *F Two additional predicted proteins with PDGF motifs are located at 27D-E on *F the second chromosome. RT-PCR analysis on ovary mRNA and sequencing of the *F product indicated that CG13780 is spliced to encode a PDGF domain protein *F (DPL2); CG13781 and CG13782 are spliced together to encode one PDGF domain *F protein (DPL3). This is noted in our manuscript. *F The ligands are predicted to have PDGF domains and are by sequence equally *F related to mammalian PDGFs and VEGFs. I think a name reflecting the *F connection to PDGF (and the PDGF domain) is most appropriate and the *F numbers useful as there appear to be several ligands (we and others have *F genetic evidence for related functions of the ligands). *F Again, the names can be changed but should not be 'VEGF'. *F Please let me know what the view of Flybase is. We will relatively soon *F submit the revised version of our manuscript. I know it is best for *F everyone if we agree on these things quickly. *F Thanks for your help. *F sincerely, *F Pernille Rorth *F From rd120@gen.cam.ac.uk Wed Aug 15 17:16:31 2001 *F To: pernille.rorth@embl-heidelberg.de *F Subject: Re: CG8222/vgr1 and PDGF *F Dear Pernille, *F thanks for your enquiry about 'Vgr1' (FBgn0032006) and its ligands. *F FBgn0032006 has the symbol 'Vgr1' because Christophe Roos named it VGR1 *F in GenBank/EMBL/DDBJ record AJ250859. The FlyBase gene record for this *F gene was brought into being labelled Vgr1 in line with our nomenclature *F policy about only the initial letter of the symbol named for a protein *F being capitalized. *F Your reasons for changing the symbol from Vgr1 seem eminently *F sensible. We could change the symbol/name for FBgn0032006 to Pvr (not *F currently being used for any other gene) or another unique symbol, but *F the new symbol/name needs to be agreed by Christophe. Ideally you and *F Christophe would send me a joint email asking for the symbol/name to be *F changed. This email would be kept as a personal communication from the *F both of you to FlyBase. I've enclosed Christophe's contact info from *F FlyBase for you. *F As far as the ligands go, it is entirely up to you how you re-name the *F CG genes, though beginning a Drosophila gene with D for Drosophila or *F Dm for D. melanogaster cannot be accommodated in FlyBase gene records. *F (I've enclosed an email we sent out about this some time ago \- for the *F sake of completion). Pvf1, Pvf2 and Pvf3 (for PDGF- and VEGF- related *F factor 1, 2 and 3) would be fine. We look forward to your paper *F because we enjoy seeing functions assigned to CGs, and merging CG *F records into one gene record to improve the annotation, whenever we *F can. *F All the best, *F Rachel. *F FlyBase Consortium. *F From pernille.rorth@embl-heidelberg.de Thu Aug 16 12:41:03 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re:CG8222/vgr1 and PDGF *F Dear Rachel, *F I've just talked to Christophe Roos and he agrees that Pvr is a good name *F for CG8222/vgr1, reflecting that this receptor corresponds to mammalian *F PDGFR as well as VEGFR, and that there is only one such receptor. I asked *F him to send an Email to Flybase to confirm this agreement. Pvf1-3 then *F seems an appropriate name for the ligands. We will make the appropriate *F changes in our manuscript and hope that the names stick... *F Thanks for your help, *F Pernille *F From christophe.roos@helsinki.fi Thu Aug 16 14:27:46 2001 *F To: 'Rachel Drysdale (Genetics)' , *F Pernille Rorth *F Subject: Receptor and factor naming convention *F Your ref: Rachel Drysdale (Genetics) *F Subject: Gene Vgr1 (receptor) and three factors naming *F We have named these sequences primarily to enable operating with these *F genes/proteins. Since we for the moment have no really solid clue as to *F their function, I am not trying to impose any naming and would be glad *F to agree to a common and more sensible nomenclature. Now, Pernille Rorth *F has contacted me on this matter, since she has been working on the same *F genes and is now submitting an article on the studies. *F In a correspondence with Flybase (Rachel Drysdale (Genetics) *F , Aug. 15th 2001), Pernille has made a naming *F proposal to which I am ready to agree *F if FlyBase considers this sensible. *F This is the present status: *F code / presently / EMBL AC / proposed *F \-------------------------------------------- *F CG7103 / DmVEGF-1 / AJ401391 / Pvf1 *F CG8xxx / DmVEGFR / AJ250859 / Pvr *F CG13780 / DmVEGF-2 / AJ312312 / Pvf2 *F CG13782 / DmVEGF-3 / \- / Pvf3 *F We have a paper in press where all these molecules are *F discussed. As soon as the coordinates are known, I can *F update EMBL and FlyBase. *F So far, if you (FlyBase) consider this naming convention *F appropriate, I can do the updates in the EMBL entries, while you will *F probably do them in FlyBase. *F Please let me know how to proceed. *F Thank you very much for keeping our very valuable FlyBase up to date. *F Sincerely *F Christophe *F Christophe Roos Dr.Sc, doc., MediCel *F Biomedicum, Haartmansg. 8 (PL63), FIN-00290 Helsinki *F phones: \+358 9 19125123 / \+358 50 5326029 # *U FBrf0137516 *a Cook *b K. *t 2001.8.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Aug 14 16:35:06 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Characterization of Tp(2;3)vg89e88 *F I have reanalyzed the polytene cytology of a Df(2R)vg89e88 stock obtained *F from the Umea Stock Center. Df(2R)vg89e88 is the deficiency component of *F the uninverted insertional transposition Tp(2;3)vg89e88 with breakpoints *F 52B3-C1;53E2-F1;68E3-F1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0137517 *a Levis *b R. *c A. *d Spradling *t 2001.8.14 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Tue Aug 14 17:38:33 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: Wrong gene assignment for l(2)k00619? *F I'm writing to suggest that the current gene assignment for *F l(2)k00619 may be incorrect. l(2)k00619 is shown in FlyBase as an *F allele of the gene capt. As far as I can tell from a cursory *F investigation, this allelic assignment was originally made by *F Spradling et al. 1999 (Genetics 153: 135-177) because the insertion *F is located just upstream to the 5' end of capt (which at that time *F was known by the name Dcap). Allan's records do not shown any *F genetic data confirming that l(2)k00619 is an allele of capt (such as *F non-complementation of an insertion mutation within the coding region *F of capt). In looking at the position of the l(2)k00619 insertion on *F GeneSeen, I noticed that it is closer to the annotated 5' end of *F CG4775 than the annotated 5' end of capt. Moreover, l(2)k00619 hits *F the cDNA SD10843, which overlaps the annotated position of CG4775 *F rather than capt. Neither the 5' nor 3' EST's for SD10843 coincide *F with the annotated ends of CG4775, which might indicate some *F adjustment is needed there, but I don't have the time or the *F expertise to look at all of the data for this. In summary, I don't *F think there's strong evidence supporting the current assignment of *F l(2)k00619 as an allele of capt. It may affect the function of capt *F or CG4775 or both. *F There is a stock for l(2)k00619 at the Bloomington stock center *F (BL-10484), so the stock center name and other information for this *F stock should be corrected too. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ *F From spradling@ciwemb.edu Tue Aug 14 18:19:44 2001 *F To: , Robert Levis *F Subject: Re: Wrong gene assignment for l(2)k00619? *F I would like to agree and amplify upon Bob's correction. l(2)k00619 mutates *F CG4775 based on its location within that transcript. It does not mutate capt, *F however. l(2)06955 is inserted within the capt transcript (Flybase genes; *F GeneSeen), and these two lines were shown to genetically complement (Spradling *F et al. Genetics 153: 135). Therefore, all public databases and the *F Bloomington Stock Center should be corrected to indicate that l(2)k00619 is an *F allele of CG4775, and that l(2)06955 is an allele of capt. # *U FBrf0137846 *a Hariharan *b I. *c *d *t 2001.7.24 *T personal communication to FlyBase *u *F Personal communication from Iswar Hariharan, 24 Jul 2001 *F *F [Curator's note: The amino acid affected in the Tsc1[R453X] allele is at *F position 460, not at position 453 as stated in FBrf0135684. There *F is an error in the amino acid numbering in figure 3 of FBrf0107789; *F the 4th row amino acid number should read 468 instead of 461. *F The coordinate for Tsc1[R453X] in FBrf0135684 was determined by *F using the figure from FBrf0107789 and counting backwards from 461.] *F *F The amino acid affected in the Tsc1[R453X] allele and the source of *F the error were verified by Dr. Iswar Hariharan in response to a curator *F query: *F *F Date: Tue, 24 Jul 2001 15:34:39 -0400 *F To: Leyla Bayraktaroglu *F From: Iswar Hariharan *F Subject: Re: question about Tsc1 mutation Tsc1[R453X] *F *F Hi Leyla: *F *F Yes - your explanation is correct. We used the figure from the Ito and *F Rubin paper and counted back from '461'. Please indicate that the Arg to *F Stop mutation affects residue 460 and also indicate the mislabeling in the *F Ito and Rubin paper (otherwise it might cause confusion if somebody tries *F to find the residue in the figure in the paper using Flybase residue *F numbers). *F *F Thanks and best wishes, *F *F Iswar *F # *U FBrf0137847 *a Pan *b D. *c R. *d Levis *e *f *t 2001.9.10 *T personal communication to FlyBase *u *F Personal communication from Dr. Duojia Pan, 10 Sep 2001 *F *F The following information was provided by Dr. Duojia Pan in response to *F curator queries regarding Tsc1[12], Tsc1[+t4.7], and Pten[dj189]: *F *F From: Duojia Pan *F To: Leyla Bayraktaroglu *F Date: Mon, 10 Sep 2001 17:47:57 *F *F Dear Dr. Bayraktaroglu; *F *F Thanks for the message. Here is the information you requested. *F *F 1. Regarding TSC1, I have attached to this message the complete *F sequence of the 4700 bp genomic DNA that I used for rescue. PCR was *F used to clone this fragment into the rescue construct. The nucleotide *F that was deleted in TSC1(12) is 3350 (an A) from this sequence. *F *F 2. Regarding PTEN(dj189), I have attached to this message a 7000 bp *F genomic DNA spanning the PTEN locus. The insertion site of the *F F-element is between 3987 (a T) and 3988 (a C). The rescue construct *F includes sequence from 784 to 6972. PCR was used to clone this *F fragment into the rescue construct. *F *F Please let me know if you have further questions. *F *F Duojia D.J. Pan, Ph.D. *F *F Tsc1 genomic sequence: *F cattattttacatcgattataacattcgcaccggaattggcaaaaagttgcgccaaaacagctgatgttg *F ttttgattgcggccaaagctgccggacattcgccagcggcgttgccagacaaaaccatcgattggctggc *F gataacagcgcaagaggaatatcacaaaaaaataaccttttcattttcaagacaaacatgtaaaaaaata *F tgagtttagcagaggtaagcattttaaatgtgcactccacaaccgaaacaatagtgcagtgcatcgaacg *F cagtgggaatttcaatgccaacccttgttctccagttcacagcgaccaagacgagaaaaagcacacgcag *F ctctgcaaaagacaaccacgacgacgagtgagtggagtagaaggaccaggagcggagtagcaggagcagg *F aacagtcgaccgagggcgggcggaaaaatcaatcatagcgacaggaaaccaataaagccgccgtcatggt *F gattgagaagatcattggtgacctggagtccaacatgacgctggagaacgaggaggccaagcgcaagctt *F gtggagttgctatcccagagtgagtcctcgccagtttcagttaatatgccacctattccaacataccatt *F tccccacagacaaggagcagtgggtggtaaagttcatgctggactacttctttacaactggatctcagcg *F cattttggaggtactggtcaaagcccaggcacctcacgatgggtacatctttgacaagctggacgactgc *F ctaaagcagtcgcagcaccgagtgcagagcctccaggtgttctgcttcattgtgcgccatcaccctactt *F ggctgtacaagatcgagaaacaccggctgatcaaaagtgtttttaagcttatgacggtgagtttatgggt *F gtttacaattttgtccgagttcagcatcacttccatttctctgacagcacgagaaggagatagttccgct *F gatgagcgccctgttgtgcataattactctgctgccgatcataccgaattctgtgcccaactttcttaac *F gatctgtttgaggtgttcgggcatttggcctcgtggaagctgcagaatagcaataaactgccggacgaga *F agctcgtccacctgcagttgggtctacagatgctatttcaccgcctgtacggcatgtatccgtgcagctt *F tattgcctatttagtggagttcatcaagcgaggcaacggcgggggcatcttccagcatacaatcaagccg *F ctgttgaacactgtgcgagtgcatcccatgctggtgacggccacgccagagactgaggtaaacaatacgc *F gatggaaggagatggagccgcatgacgtggttatggagtgcgccaacctatcgctgcccgtcctcttgcc *F cgagacgagcaacgaagacggcagctatgcgtatcccatgacgccaggatacagtcgcatgacttcaaat *F acctcgaatacggactacagctatcagctgagggagtttcagcaatcgagaaatgtctacacccgcttcg *F attcgtttgcctcgggtgatgatgtgggtccgatctggagtccgcataacgagattgccacgaccagtag *F cggcataccgcttacacccaccacatcgtttattttgccactacaaccggctatgaactctcagcttatg *F gttggcatgactggctcctcaccgcccgaagcagctgtggaggccacaccggaaaccacccccttaaagg *F atatgagggatatcaagcagccgggacgtgcggtcaattcgcatgctgtgagagctatcttcgccgtaag *F ccagccttcttcacccatgcgcaaggaccagcagagtcagttcagtttcccggatgtctctcgcgaggcg *F gaggagagcagccactcatatctggaggtcaacagaggaactgcctatgaccgtcgcctgtcgcaggtca *F tccaggacaggcataacgtggagcgatctgtaaacacaccttgtccaagcagcctgccagaaattaactc *F cgatttatcccttgttggtggttccgtctatccatctgtcacgcaggaggtcgctgcagtttgtggcgag *F tgcaacgagacggataggaacctctgcagtgtgggtggacttcatatgcccaccagccgatccatgcacc *F agctggcaaagaagcgccgcaatcgtatggcaagctacagtggaaatggttcctgtgcggacagcagaag *F ttcggcggcaaagaaggcaagttggagtactgaggcagagaacccaatgcgacgaaccaaatcctgctcg *F gccctttccggaatgcggcagcagcatctggaggagaatgatgacgaggccgattgttcgagccaaagac *F aaagaggggagaatggaaatacgcaaaagactggcagccgcctgcagaggagcggccggaacctggccat *F ttcggcgcccaaggatacggctagaagctgcacccatgcctccacccagacggtggaaggactggacagt *F gctccagcgcagtacgagaattggcttattgaactcctgctggagtgcaaggagcaaagaatcgactatg *F aaaggaaccttctgtacccgcaagatattctagacgaatacattaagcatgcgatcaaggccaatgagtc *F ctttgacgccgagcagggtcaactgatgtgcctacagctggaatacgaaagctaccgtcgatccattcac *F gcagagcgcaatcgacgactcatggggcgaagcagggacaagcgcagcctggaaatggagcgggatcggt *F taagggagcagcttaagaacttcgatgcgaagaacaaggatctggcaaacaaaatggatcaggccattcg *F gttggccaacgagcgccagaacatccaccaggaggagctgggcgaaatgagggctaagtaccagcacgaa *F ctggaggaaaagaagtgcctgcggcaggcaaacgatgacctgcagacgcgactcaccagcgagttggcgc *F gccacaaggagatgaactatgaactggagtctctgcgaggtcaggtcttcagtttgggaaccgagcttca *F acacacccagcagcaggcggacattgggctgcagtgcaagcaggagctggcacgactggaggccgagttt *F attatcatgggtgaggtgcaagtgcgttgccgcgaccgtctggctgagatcgataacttcagggcccgcg *F acgaggaactgcagatgctacaagagagcagcaacctggaactgaagggtgcctattcattgtttatatg *F atacggatgtttaatcattcagtttatttattaagatctgaggcacagcctggacgagaagacatcacag *F ctggaaagcatgaagcacaagatcagcgacctgcaggcccagctagccaacagcgagaaggccatgacgg *F agcaaaagcgacttctaagcaccgtcaaggatgagtacgaagaaaagtttaaggtagggctgatatttaa *F aatcgtatacttatctgaaatagttcggacttttaagaaatagtctgtgtttaccagtaacttcatttca *F attaagagtaataagccttaaaaatcgtcgtccttgcagtccgtgaacaagaagtatgacgtgcaaaaga *F agataattatgcagatggaggagaagctgatgatgatgatgcagcagccgcaaggaacaacaggtcataa *F cacctgttccccggacacggacagaactggtgagtgcaagagctgcttcgcagctaaagaatacttacta *F ttgcattctcccacagacatagcttcatccattgaacgcaactcaccgctatccacgtcgctggcctcga *F gcgagagcttatccgccagcctacgctccacggagctgaagaacctgcaccagctagtggacacgcccac *F tattccggatgtgctgaacagcatggccggtggcgctcagttcgaggacgaagtgcgtccgccggccgtg *F gatctggcctcctcggcaagcaccgccagtgccatcaacatcgtgccgcacgccttggacttgccgtcga *F cctccggcggcatcggtcacacgctcacccacccacatccgcatccgcacctgcacctgcagcaacagca *F acaggatcaactgcagtagtagcagcagacgcagtttgtaaaaacaaataagaactagctttccttcgct *F aaacaaatgtctattgggaaaacccgcacagaaaatttacaaaaaaaagttattggttcttcatattttc *F atattcagttcaaaactgtaactaaggtttaataaatcgctgactccacgcaataattataaaggttata *F aaaaatattaatgtttgaaattttaacttgtttagcacattgcaatttgttgtaaaaaagtggttttgat *F acgaggcaataagtaagacgcgcattcatattataataaacatttgattaagatcagcaaacataaaagc *F attgttttattttatttaattgtaaacatactatttgttatataacaaccgccaaacatttaaattttag *F ctaagagagcaaacaaaaacgtaaacaaaccgagcgacattgccagctgaaagaaagtgagaagtgtggc *F aaccttaaacgaaacagctgagcggaagcaacaataatatagaaagaagaagcagaaaagaaacgcctat *F taaatcagat *F *F Pten genomic sequence: *F gtgcgacatactgaacggatccgagctgctcggctcccagctgcgcgtggagatctcaaaagggcggcca *F cgccagggtaggcgtggcggacccatggacaggggcggacgacgcggcgactttggccggcacagcatca *F caagcggtggtagcggcggaggcggtttccggcagcgcggatccagcggatcctcaagccggcacacgga *F gcggggctatagctccggccgatcaggtgcaagcagctataatggcagagagggcggcggcagcggcttc *F aatcgccgcgaggtttacggcggtggacgcgacagcagccgctacagcagcggaagtagcgccagctacg *F gacgcactggtggtcagtcggccggacgcttcaggtcccgctcgccggtgggaaaccatcgattctaatg *F acaacaccactcatagttgcttaaggattgcctatgagtaaagattaataaataataacttaagcgcgac *F cgtaaaacgcagactcaaacatttaaaatcgtagcattcgatcgttttcgatcgtccaacagattccctc *F attcccccacatcaacaacaacaacagaatgataaaaaaaaaagaaaaaacaaaaaactgatagtcgtcg *F tctcttcattatttatttaacgaatacccattaacatgacaattgcaagcacgtaaaatttaagttataa *F cgtagaaaactagtaagaaaaatacgctaagactcgaacaacgtcaaaccaaaatctagaagtacgcaat *F tttaataaaggaaatgcggaacagtttctcgtatacataaaagctttcgcaaaataattggtctaaaaca *F ttaaagaattttgagaactcggaattgtgtaggtgttcatttttacagaacctcttataaccatcgtaat *F aaaccctacgaaatgaagcagtcagcagtccgaaactggtaagaaagcgttttcgaaaatctgctttgtt *F ttctgaatctcgaaaacctaaaaagcaataaccgccaagtgtttacaaacaaatcattgactttacgttt *F tggtaagaaatattgcatgtgtactatccatcgatctaactttttacctattaagacaaatactatacaa *F atctataacttattgtagttgggtcgaaacggcaaaaatctagccaatgctgcaagcaactatattaagc *F gcatcggttttaatgtttatgttttatttcatttaactaaattaacaggtcatttgttggagtatctctt *F aacgtcactttcagatttattagattgcttaaagttcaaaacatagctaagcaatacttatgataacgac *F gattagttagttagcccaaggggctattaaatatttaaacgatggactgcagaaactttttaaacaaata *F aaaagcgagacaaataaatgagaaattggttaacacggcaaaacacttgtttttatttcttcgtcttcga *F gtcctttttgcttcaataacatttactgatgattggcctattttgatttattcaagttacgaattaaagt *F caacagttgagaattaagtggtaccaagaattcattttgcggaaaccaactagccttaagatacgagtgt *F aatttttaaagttttgaaaattttttaaaattttaaattattcagatagcaggttttgcttttgaataaa *F ggaactttaaattccaatcatatttatgtcaaaataatcgaatacccataaaaaacctgttgcactttta *F tcgattgtaaataaaaaaagagaggattacattaatggtattacccaaactgatagttttcaatagcatt *F tcaatggctggaaaaaaaaataaataaaaggtattcgtaaatcttaaaaatgtttgcgttttaaaaaatt *F gcataccaaaaataatattggttttgcaagaaatggaatttttaatagctgtagcaatattagtttcgtg *F ggtagaactcgagtaaaagatgtgttggtagtcatgtttttggtagcatgaagttatatgtgtgttgttt *F aataagattaaaataaatgcatagataattacatattgtttaaaaccgcagtgctaaaaaataaatggtt *F gtatcgatgacccatcgatagtttttgcatacacaatttaatcgagagggcaccacgcacgtcgatagca *F ctggatgtacattttctcaaatcttgtggtggtatcgctcgccgtaaacaccctgtggatctcacgcttg *F ataaattaataatacatttaatagagcatgagcgctactgtttaacttttacacataattgaaaagtttt *F atttgaaatttcgtggcttagagcaaaacatgtgatagctttttgaaatgcagtagccagaacctgctcg *F gttggaacatttagcttattgacttaaacatacccatatgcacatacatacatatgtattaaacagtggg *F cacatatattcgtgtggtgctgatagtgtaaatgtgtaaaatgtctgtggaattgtaaatatgtttttgt *F ttaaacaaatatgtacataaatgcacaaaaatcggtgcatatatgtgcacatgcccatcatccatatctg *F catatttctgtacatgcatatgtgctctaactacaatacataccaccattcgcaaacagtttgtcaaaaa *F agtgtgtctactcggcgagataaaacaaaaaacagttgccaaatcagtcgctatgttaagtaattcgaaa *F ttactgctcggtgtttctaaaacggattttgattttgattttcacccctcgaaaatcgccccctcagcca *F acgaacccatttaattgctcgaattcgtatcgaaatcgccgtgcgttgggcttcaataaaagaactctgt *F tctgttagccagttacaattaaaaccaggaccatttgacaaccaatctgaatgtattatgccaagcggaa *F gattgttatattgtttatctttgataatatgccgatacatgtaagtatgtttcacgtgtgtgccgcaaat *F gcctgcctgtgtgtgtgtgtgtgtgtatgtacatatgtgcgtgtgcatgtgcgagtgtgtgtgagtgtgt *F gcatgtttgtgctaaggatatttgtggatatataaaccgaactgaagttaactctttacctgttcctggg *F aaaacaagtagggaactattcctatagagagtgcacctctgtctgtatgtgtatgtacgtgcttaaatat *F gtatccaagcaagaactgcaaacaggaatatatcttagccgagtaagaagtattagtttatatagaacaa *F cctaagtggatcgggagaaaacaagcccattatattagttccatatatccctttagaatgccttggcaca *F gtataattaagcagtttctattgaccatattagaaacttgtctgctgttaaaggatttaggtttgaattc *F gcagtagaaaactaagctgaattgagtcaatacttttctaacttgctgtggaaaataaacagattagtta *F gaatcatatgcatattaattaatatgctttacattcctttcagaacgtacactttgcaatggagtaaata *F gaagacaagcactggttcccaaatacatttgaatggatgcagctaatctaatttaaataaaaaagacaag *F tctttaaaccttaaaaatatggccaacactatttcgttaatgtccaacgtgatacgcaatgtagtgagta *F aaaaacgtatacgatacaaagaaaaaggatacgatttggatttaacatgtaagttattgaatcaaccgca *F atcattggaatattaaattaaattatctatttacccacagacattaatgataacataattgccatgggct *F atccagcaccggataaactagagggcctattcagaaaccgtctggaagatgtctttaagctattggaaga *F gaatcatgcccagcattacaaaatctataacctatgctcggagcgtagttacgacgttgctaaatttcgc *F ggggtaattttgttgaactttttctatttgtcggcgtaaaatatgtaaaatatactatttcgcattacag *F agagtagctgtttatccgttcgatgatcataaccctccaacgattgagttaatacaacgattttgcagtg *F atgttgatatgtggctcaaggaagattcgtccaatgttgtagccgtgcactgtaaagctggaaagggcag *F aaccggtaggaaatgtgttcaacattttgtgcgaaagttcataaatatcatggcacactatgatcttgtt *F tcgatatttgtagggctaaaaaactaacccagttttttttcatttcaggtaccatgatctgcgcatattt *F agtgttcagtggaattaaaaaatccgccgacgaggcattagcgtggtatgatgagaagcgtacaaaggac *F cgaaaaggtgtgacaatcccatctcagcgtcgatatgttcagtatttttccaaactagtttgttcaagtg *F ttccatattcgaaagtaagccttaacgtgagtatttccagcagcagcgccacattcagcgccgtacaaaa *F tctaaatatgcattttttgtatctcaggtatgtgaaatacgcttctcggagtccagttgtgtgcaaaact *F taggcatggtagaatgttcaatatctgtattacacgactcagccacagaaaatgcaaagccagatgtaag *F tatataaagtatatagatacatcattgatagactatagacagcattttaaaaatgtatatttcacacgat *F ttcagtgcagtaaagtgttccttttcgaatacctttctaaccagcagtaacattcttcttttttttataa *F ttgcagagactgaaaaccttgccgattgattttcaaaaatcatttgttttgaccattaaaccatcaatac *F cagtttccggcgatgtaaaatttgaactcacaaaaaaatccccagataaaatcatttgtcacttttggct *F aaatacgttttttgtacgaaactattcacgtaagtatacttaatatcgcacatgcattaaataaacgatt *F taattaaacattctctttagcttgcgagtccgatggaacagttaacaaatatatacacactttaagcaaa *F tcagaaatcgatgatgtacataaagacagtgaacataaaagattttcagaagaattcaaggtaggatatt *F tcgaagcattttaatagatgctgcgaaattaccaaataaatgtgcttgctctcttttcagatttcaatcg *F tttttgaggcagagaattttagcaatgatgttcaagctgaagcgtccgaaaaagagagaaacgaaaatgt *F tttaaatttcgaaagatcagactatgacagtttgtctccaaactgctatgccgaaaaaaaagtcttaact *F gcgatcgtcaatgataatacaacgaaaagtcaaacaatagaaacgttggatcataaggatatagttgtaa *F gtgaaaacacgttttgcgccgatcaatatgcttaatctataaatctaatattcgtagactaaaattcaat *F acgacacatcaaccaattcaaaaaatacttcgactgcgtgcaaacgcaaacagcctaatagcaaaactct *F tttaccaagcttgaatgattccacaaaagaggaaataaaacgaaatcatatatttaatcaaccgtccatt *F aagaaaactgatttaatcaaatggcagaatagtgaagttcatatcacctctgacactcgaagtataaacg *F aaaataagaacatcaattacaattcatacatcacatgtaaacaatcctctccaaaattcaactgtggtac *F cgaagatggcgaggaagattgggaatccggtgagtttgctgcacactatactctcaatgaccagatctca *F ctaaaaatctatccgttttaatcgagtaataccgaacaattgttctaatttcacatttatttaatactaa *F gagtttcgattcaattgatttctggaccattatcggtttgattttgtaattaattgtattgtatgagtat *F tcaaattaagatttgttaaaagagatagcctttatcgacatatgctatcgtccacacatttatattctta *F ttgacttattgaattcgccagaataatcacccgagacgatttaaaagacttctggctgaacgatctctta *F attttgatgtataaaacgaagccaccagaactggatttatatgtccccaagacttttacgagtatactag *F cagagacaggcttacacagatccacttcctttcacggggaaacgatcgattacagctgcgctctgcacat *F ctgtttagaatttaatgtacataggtgagtgagtgggagcccatgcctgcggctcgaatggtcaactaac *F cggcaatccttaatcagtatccgcatcgattttcatcacacatccctcaccgcttcataagtatgtgact *F ctgggcactgccattgtccttctactcaattcatttaacaaaactagcaccgaactgtcgaacttcgact *F aattcgactatttcactgttgctgttgaaaactaaatatcctgaagcagaatgtgtcttttgtgtgtttc *F atcatcatcaaaagtcttctgcttttaattcattcatgctattttgatttttatcgtattctcgttgagt *F tttagttaataaattcattaagcttgtttgagcaaaaaaatctaaactaataaatgaatatttttcttta *F tttccaggtgaatcaacatacctgtaatcaatgcttattacggacttataattcttggtgatttagcaaa *F gtaattaaaattgctaagtaattggctaatctattcgaatttgtaaatatactgtatgatatttatttaa *F gtagttttaacaattattttaaataaactcatgtacattttgtattgtagatttcattcgaactttgggt *F ttttgtctaaagtaaggggggagaaataattgacgtttagaaaaaaatacttaaatttatagttttgatc *F # *U FBrf0138556 *a St. Johnston *b D. *t 2001.8.23 *T personal communication to FlyBase *u *F To: Chihiro Yamada *F From: Daniel St Johnston *F Subject: Re: FlyBase Query (cy1359) *F Date: Fri, 24 Aug 2001 12:08:18 \+0100 *F Hi chihiro, *F . *F The lethality associated with P{PZ}rL203 is not due to the insertion, and *F Freek recombined it off before mobilising the P. *F . *F Dr Daniel St Johnston *F Wellcome/CRC Institute *F Tennis Court Rd *F Cambridge CB2 1QR *F United Kingdom *F Tel: (0)1223-334-127 *F Fax: (0)1223-334-089 *F To: ds139@mole.bio.cam.ac.uk *F Subject: FlyBase Query (cy1359) *F From: Chihiro Yamada *F Date: Fri, 24 Aug 2001 11:26:20 \+0100 *F Hi Daniel! *F I'm currently curating your paper for FlyBase: *F van Eeden et al., 2001, J. Cell Biol. 154(3): 511--524 *F P{PZ}rL203 *F ========== *F This insertion is in the 5'UTR of btz. On page 515 you tell us that it *F 'does not cause a phenotype'. Does this mean that the homozygous *F insertion does not cause a btz phenotype or does not cause any *F phenotype at all, and is thus wild-type? *F I need to know because currently this insertion is in our records as *F causing an allele l(3)rL203rL203, which is stated to be homozygous *F lethal. If there is a real discrepancy between the phenotypes here *F then it suggests the lethal phenotype is separable from the insertion *F and will need some extra sorting out. *F . *F Thanks *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \---------------------------------------------------------------------- # *U FBrf0138560 *a Lopez-Schier *b H. *t 2001.8.25 *T personal communication to FlyBase *u *F Date: Sat, 25 Aug 2001 22:56:20 \+0100 *F To: cy200@gen.cam.ac.uk *F From: Hernan Lopez-Schier *F Subject: Ecol\lacZS138716 *F Hi Chihiro, I was just looking at the entry in Flybase of our *F latest paper and realised that the LacZ lines that we used are cited. If *F you are interested, I have additional information which by the time we *F published the paper was not available. I rescued the P of the line *F lacZS138716 and sequenced the flanking genomic sequence, and found out that *F the insertion is in the 5'UTR of the gene misshapen (msn). It also fails to *F complement other msn mutant alleles. *F Cheers, *F Hernan.- *F Hernán López-Schier *F (St Johnston lab) *F Wellcome/CRC Institute, *F University of Cambridge *F Tennis Court Road, *F CB2 1QR Cambridge; *F United Kingdom *F Tel 00441 223 334113 *F Fax 00441 223 334089 *F http://indigo1.welc.cam.ac.uk/~dstjlab/ # *U FBrf0138561 *a St. Johnston *b D. *c D. *d Micklem *t 2001.8.27 *T personal communication to FlyBase *u *F Date: Fri, 24 Aug 2001 17:21:15 \+0100 *F To: Chihiro Yamada *F From: David Micklem *F Subject: Re: FlyBase Query (cy1359) *F >Hiya David, *F > *F >I got a quick work question, and a test of your memory/records system. *F >I remember that you were pretty thorough with records of your molecular *F >biology, so I have faith you'll be able to answer this one. *F > *F >I'm currently curating: *F > *F >van Eeden et al., 2001, J. Cell Biol. 154(3): 511--524 *F > *F >In it they use GFPmago17.1, which is one of yours... can you give me *F >some molecular details about this and I'll make it a PC from you to *F >FB. *F > *F >Thanks *F > *F >Chihiro *F > *F Hi Chihiro, *F Hmmm, it isn't using the approved Micklem nomenclature so its a *F little difficult to be absolutely sure. Also, most of my notes are *F still in Daniel's lab. *F I've had a quick look at my thesis \- and as I thought it _definitely_ *F isn't in there. In fact I have a vague and sneaking suspicion that it *F was actually made by Stefan (Grunert). *F .. *F David *F \-- *F __________________________________________________________________ *F D.R. Micklem, Time flies like an arrow... *F Dept. of Genetics, Fruit flies like a banana. *F Cambridge University, Email:drm21@mole.bio.cam.ac.uk *F Cambridge, UK Phone: \+44 (1223) 766336 *F Unsolicited email will incur a US$100 processing charge. *F __________________________________________________________________ *F Hi chihiro, *F .. *F I can answer two out of three of your questions, but can't find the details *F of GFP-Mago, even in David's thesis. My recollection is that it is in our *F standard maternal alpha4 tubulin vector and has GFP fused to the N-terminus *F of full-length mago, but you might want to check with David. *F .. *F Dr Daniel St Johnston *F Wellcome/CRC Institute *F Tennis Court Rd *F Cambridge CB2 1QR *F United Kingdom *F Tel: (0)1223-334-127 *F Fax: (0)1223-334-089 # *U FBrf0138563 *a Reichhart *b J.M. *t 2001.9.14 *T personal communication to FlyBase *u *F Date: Fri, 14 Sep 2001 14:03:18 \+0200 *F To: Chihiro Yamada *F From: JMR *F Subject: Re: FlyBase Query (cy1375) *F The BamHI site was introduced in ccgcaggaTccaaatccc, 64bp *F downstream of the tatabox of YP1. So it's an YP1 promoter fusion. *F .. *F Jean-Marc *F \------------------------- *F >Hi, *F > *F >Thanks for your reply. I just have one point I need to clarify. *F > *F >So you have used a the piece of genomic DNA from between the YP1 and *F >YP2 genes. Does this fragment have exon 1 of just YP1 or both? If it *F >has both, which gene was fused to the GAL4 i.e. which gene is the BamHI *F >site located in? *F > *F > > A BamHI site was introduced 64bp downstream of the tatabox of the YP1 *F > > gene (LOCUS DMYOLK1 1677 bp DNA INV *F > > 07-NOV-1997 *F > > DEFINITION Drosophila intergenic spacer and exon 1 from YPI and *F >YPII gene for *F > > yolk protein. ACCESSION X01524 M11170, prom Yolk-prot1). *F .. *F >Chihiro. *F Date: Fri, 14 Sep 2001 10:32:13 \+0200 *F To: Chihiro Yamada *F From: JMR *F Subject: Re: FlyBase Query (cy1375) *F >Hi Chihiro, *F Sorry for the delay, but you know how it is...The Gal4 line *F was constructed here. *F A BamHI site was introduced 64bp downstream of the tatabox of the YP1 *F gene (LOCUS DMYOLK1 1677 bp DNA INV *F 07-NOV-1997 *F DEFINITION Drosophila intergenic spacer and exon 1 from YPI and YPII gene for *F yolk protein. ACCESSION X01524 M11170, prom Yolk-prot1). This *F Hind3-BamHI(new) fg. was fused to BamHI site 5' of the Gal4 coding *F region of pGATB (Brand & Perrimon) . The whole fusion was cloned into *F pCasPer as an EcoRI-NotI 4192bp fg. and injected. *F The sequence of the yolk casper vector is available. I can send it to *F you or people can ask it from me. *F The expression is fat body in female only which is what I was *F looking for. By the way, I received a lot of requests for this line *F and I'm still amplifying it before giving it away. I think that I *F will send it to Bloomington. *F Hope this helps... *F Don't hesitate to contact me if needed.. *F Take care *F Jean-Marc *F \-------------------- *F >Hiya Jean Marc, *F > *F >I'm currently curating: *F > *F >Vidal et al., 2001, Genes Dev. 15(15): 1900--1912 *F > *F >In this paper Vidal et al. use a GAL4 line they call yolk-GAL4, for *F >which they cite you as the source. We don't have a GAL4 line with that *F >name. Can you identify this line? Has it been published before? If *F >so under what name? If not, can you give me some details about this *F >line. For example; Is it an enhancer trap? What gene is it inserted *F >into? Any new information you give us will be curated as a personal *F >communication from you to FlyBase. *F > *F >Thanks *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >---------------------------------------------------------------------- *F Jean-Marc REICHHART *F UPR 9022 CNRS IBMC *F 15, rue Rene Descartes *F 67084 \- Strasbourg Cedex *F FRANCE *F JM.Reichhart@ibmc.u-strasbg.fr *F phone 33 388 417 034 *F fax 33 388 606 922 # *U FBrf0138570 *a Davis *b T. *t 2001.4.23 *T personal communication to FlyBase *u FlyBase error report for CG15284 and CG3474 on Mon Apr 23 02:12:23 2001. *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 23 10:12:28 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 23 Apr 2001 10:12:28 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 23 Apr 2001 02:12:23 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@cardiff.ac.uk *F Subject: FlyBase error report for CG15284 and CG3474 on Mon Apr 23 02:12:23 2001 *F Content-Length: 7675 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: CG15284 and CG3474 *F Release: 1 *F Missed gene *F Comments: I have some annotation information for the genes CG15284 and CG3474. *F The predicted gene CG15284 lies on 35A close to (and probably proximal to) the *F chromosomal aberration In(2LR)el6 and distal to the deficiency Df(2L)el16 *F i.e., is most probably between these two breaks. (note that the two *F aberrations and CG15284 all map to the same 5.4kb EcoRI fragment) *F Deletions which remove DNA proximal to el6 (e.g. the synthetic deletion *F el6Left-A379Right) are mutant for the pupal (pu) gene, whereas the el16 *F deletion (goes proximal) is not mutant for pu. This indicates that the pu gene *F is between these two breaks (they are approximately 3 kb apart). *F I noticed this putative transcript a couple of years ago and have wondered if *F it could be the pupal gene (I realise that Mike Ashburner and I might disagree *F about this). The source of disagreement is due to the map position of *F Df(2L)el14 (I think I erroneously mapped it: see Davis, T., Trenear, J. and *F Ashburner, M. (1990) The molecular analysis of the el-noc complex of *F Drosophila melanogaster. Genetics 126: 105-119.). If the published position of *F this break is correct then it would delete CG15284 and el14 is not mutant for *F pu. As the genomic sequence is available I now think that el14 and el16 are *F broken very close to each other and neither would delete CG15284 (of course I *F am not in a position to remap this break which would clinch the matter). *F If CG15284 is indeed pupal, then CG3474BG:DS06238.4 (this is Ashburner’s *F guess for pu) is not pupal. This would fit the genetic data as both el14 and *F el16 delete CG3474 and neither are mutant for pupal. *F Then CG3474 could be a gene which we labelled 'crippled leg' (see Davis, T., *F Ashburner, M., Johnson, G., Gubb, D. and Roote, J. (1997) Genetic and *F phenotypic analysis of the genes of the elbow-no-ocelli region of chromosome *F 2L of Drosophila melanogaster. Hereditas 126: 67-75.). When this 'locus' is *F deleted, or affected by aberrations, the surviving flies have a crippled leg *F phenotype (e.g. Df(2L)el16/Df(2L)b83d29a which only homozygously deletes *F CG3474 is pupal+ elbow+ crle-). There are as yet no point mutations for *F 'crle'. The existence of this gene relies on the phenotype from overlapping *F deletions. *F If CG15284 is pu and CG3474 is 'crle' then the molecular data fits the genetic *F data better than assuming that CG3474 is pupal. *F Having stated all the above I have no actual proof as it depends upon My *F having mis-mapped the aberration el14 (I am fairly sure I did as the EcoRI *F fragment I used for the mapping (taken from the genomic sequence) is clearly *F at odds with my published position). On the other hand if CG3474 is pu then *F homozygously deleting this region does not result in a pupal phenotype? This *F is despite the observation that heterozygous combinations of deletions can *F indeed be pu- (e.g. Df(2L)A400/Df(2L)b83d29a) *F The genetics to support this are: *F Df(2L)b83d29a breaks proximal to CG3474 and deletes this locus (is pu- crle-) *F Df(2L)b83d29a heterozygous with Df(2L)el14 or Df(2L)el16 is pu+ el+ *F crle-would delete only CG3474 *F Df(2L)b83d29a heterozygous with Df(2L)fn2 is pu+ el+ crle- (fn2 is possibly *F broken in the promoter region of CG3474: this has a weak crle phenotype) *F Df(2L)b83d29a heterozygous with Df(2L)TE35BC8 is wild-type (BC8 is broken just *F proximal to CG3474 and fn2) *F Df(2L)b83d29a heterozygous with Df(2L)ARR1 is wt (ARR1 breaks proximal to fn2 *F or BC8) *F Df(2L)b83d29a heterozygous with deletion el6Left-A379Right is pu- crle- *F deletes both CG15184 and CG3474 *F Genetic data from: Davis, T., Ashburner, M., Johnson, G., Gubb, D. and Roote, *F J. (1997) Genetic and phenotypic analysis of the genes of the elbow-no-ocelli *F region of chromosome 2L of Drosophila melanogaster. Hereditas 126: 67-75 *F Thus the 'crle' gene is between the breaks of Df(2L)el16 and Df(2L)TE35BC-8 *F and homozygous deletions of this region are semi-lethal with the survivors *F having a crippled-leg phenotype. *F The locus CG3474 (crle?) encodes a putative pupal cuticle protein *F The gene CG15284 (pu) has a low degree of homology to the 3’ end of the human *F mucin genes and the growth factor for human Norrie’s disease (see below). The *F domain of homology is a cystine-knot domain. I appreciate that the overall *F homologies are low, but all the conserved residues required for the *F cystine-knot are 100% conserved (see Meitinger et al., 1993, Molecular *F modelling of the Norrie disease protein predicts a cystine-knot growth factor *F tertiary structure, Nature Genetics, 5: 376-380). The cystine-knot is required *F to fold in a specific way and to cross-link units. Meitinger et al., state *F that the overall homologies of this class of proteins is 10-30%, the essential *F requirement is for the cystine-knot domain cys residues (the pu homology is *F greater than 10%). *F The putative pupal protein has reasonable homology to the cystine-knot domain *F and all the required cysteines are conserved. In addition the pupal protein *F would have a good signal peptide indicating an excreted protein. If this match *F is more than coincidence, then pu could be a Drosophila version of the *F cystine-knot and thus be a homolog of a member of this growth factor family. *F I didn't mention the putative CG15284 earlier as it only as a low Genie score. *F This is the cystine-knot domain sequence (assume pu is CG15284) muc are the *F human mucins *F pu MHVQELLFVAAILVPQCLRALRYSQGTGDEN *F pu CETLKSEIHLIKEEFDELGRMQRTCNADVIVNKCEGLCNSQVQPSVITPTGFLKECYCC *F muc6 CS--------VREQQEEITF--KGCMANVTVTRCEGACISAAS-FNIITQQVDARCSCC *F muc2 CSTVPVTTEVSYA----------GCTKTVLMNHCSGSCGTFV-MYSAKAQALDHSCSCC *F muc5 CAVYHRSLIIQQQ----------GCSSS-----CRGNCGDSSSMYSLEGNTVEHRCQCC *F muc5B CQCRINTTILWHQ----------GCETEVNITFCEGSCPGA-SKYSAEAQAMQHQCTCC *F \* \* \* ^ \* \* \*\* *F pu RESFLKEKVITLTHCYDPDGTRLTSPEMGSMDIRLREPTECKC--FKCGDF----TR *F Muc6 RPLHSYEQQLEL-PC--PDPSTPGRRLVLTLQVFSH----CVCSSVACGD *F Muc2 KEEKTSQREVVL-SC--PNGGSLTHTYTH-IES-------CQCQDTVCGLPTGTSRR *F Muc5 QELRTSLRNVTL-HC--TDGSSRAFSYTE-VE-------ECGCMGRRCPAPATPSTR *F Muc5B QERRVHEETVPL-HC--PNGSAILHTYTH-VD-------ECGCTPF-CVPAPM *F \* \* \* \* *F This is the 3'terminal of the mucin proteins. The cys residues make up a *F cystine-knot and are involved in the oligomerisation of the mucins. ^ is an *F important conserved Glycine *F Norries disease protein The first amino acids are a signal peptide *F NDP MRKHVLAASFSMLSLLVIMGDTDSKTDSSFIMDSDPRR *F pu MHVQELLFVAAILVPQCLRALRYSQGTGDEN------- *F 1 a 2 3 *F NDP CMR--------HHYVDSISHPLYKCSSKMVLLARCEGHC-SQASRSEPLVSFSTVLKQP *F | : :||: : | |:: :||| | || |: | *F pu CETLKSEIHLIKEEFDELGRMQRTCNAD-VIVNKCEGLCNSQVQP-----SVITPTGF- *F b \*4 c 5 6 d *F NDP FRSSCHCCRPQTSKLKALRL-RC--SGGMRLTATYRYIL--------SCHCEECNS *F :: | ||| | | : | :| | ||| |:| | *F pu LK-ECYCCRESFLKEKVITLTHCYDPDGTRLTSPEMGSMDIRLREPTECKCFKCGDFTR *F The conserved cys residues are numbered and form cys-cys bonds as follows: *F 1:4, 2:5, 3:6 *F The cys marked with \* is essential to cross link monomer units. *F The degree of cross homology is similar to that seen between human TGF-b and *F NDP, the spacing between the cys residues not too important. *F Note that this is the same level of cross-homology as between other *F cystine-knot domains *F The same region is conserved in von Willebrand Factor *F Yours sincerely *F Terry Davis # *U FBrf0138573 *a Rabouille *b C. *t 2001.8.23 *T personal communication to FlyBase *u *F Date: Thu, 23 Aug 2001 15:26:58 \+0100 *F To: Gillian Millburn (Genetics) *F From: Catherine Rabouille *F Subject: Re: FlyBase query: EDRC2001: O11 *F >Dear Dr. Rabouille, *F > *F >I am curating your abstract for the upcoming European Drosophila *F >conference in Edinburgh, for FlyBase. *F > *F >I am writing in connection with your abstract: *F > *F >O11 'Analysis of Golgi protein mRNA expression during Golgi stack *F >biogenesis in vivo in Drosophila melanogaster.' *F > *F >You mention 3 genes that are new to FlyBase: 'dERGIC53', 'p47' and *F >'Golgin84'. *F > *F >In each case, do you know which of the Genome Project CG annotations *F >the gene corresponds to? All the CGs have corresponding gene records *F >in FlyBase already and we don't like to make duplicate records for what *F >is actually the same gene unless we can't avoid it. If each gene does *F >not correspond to a CG then perhaps you could tell me the map location, *F >as this is valuable information for the genome annotation project. *F > *F >Any information you give me will be curated as a personal communication *F >from you to FlyBase, *F Dear Gillian *F >You mention 3 genes that are new to FlyBase: 'dERGIC53', 'p47' and *F >'Golgin84'. *F dERGIC53 is actually 'Rhea' (CG6822) and is at 66D5 *F p47 is CG11139 at 43C4-5 *F Golgin84 is CG17785 at 95F9 *F Hope that helps *F Catherine *F Catherine Rabouille, Ph.D *F Wellcome Center for Cell Biology *F Institute of Cell and Molecular Biology *F The Michael Swann Building, Rm 4.19 *F University of Edinburgh *F Mayfield Road *F Edinburgh EH9 3JR, UK. *F Tel: 0131-650 7125/7073 *F Fax: 0131-650 7360 *F Email: C.Rabouille@ed.ac.uk *F Web page: http://www.icmb.ed.ac.uk/staff/rabouille.htm # *U FBrf0138574 *a Shirras *b A. *t 2001.8.23 *T personal communication to FlyBase *u *F From: 'Shirras, Alan' *F To: 'Gillian Millburn' *F Subject: RE: FlyBase query: EDRC2001: I12 *F Date: Thu, 23 Aug 2001 15:32:14 \+0100 *F Dear Gillian, *F dNEP1 is CG5894 *F dNEP2 is CG9761 *F dNEP3 is CG9565 *F Best wishes, *F Alan *F \-----Original Message----- *F From: Gillian Millburn mailto:gm119@gen.cam.ac.uk *F Sent: 23 August 2001 15:06 *F To: a.shirras@lancaster.ac.uk *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase query: EDRC2001: I12 *F Dear Dr. Shirras, *F I am curating your abstract for the upcoming European Drosophila *F conference in Edinburgh, for FlyBase. *F I am writing in connection with your abstract: *F I12 'The Neprilysin (NEP) family of zinc metallopeptidases *F \-implications for peptide signalling in Drosophila melanogaster.' *F You mention 3 genes that are new to FlyBase: 'dNEP1', 'dNEP2' and 'dNEP3'. *F Do you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F Any information you give me will be curated as a personal communication *F from you to FlyBase, *F Thank you for your help, *F with best wishes, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0138576 *a Staveley *b B. *t 2001.8.23 *T personal communication to FlyBase *u *F Date: Thu, 23 Aug 2001 17:51:47 \-0230 (NDT) *F From: Brian Staveley *F To: Gillian Millburn *F Subject: Re: FlyBase query: EDRC2001: H7 *F Dear Gillian Millburn, *F Identification of Dfoxo represents a continuation of my studies of *F apoptosis and cell survival signaling with an emphasis on the akt pathway *F (see Staveley et al., 1998. Genetic analysis of protein kinase B (AKT) in *F Drosophila. Curr Biol. 1998 May 7;8(10):599-602). The foxo subfamily *F (forkhead-box subclass 'o') of transcription factors (formerly FKHR, *F FKHRL1 and AFX) possess three potential akt phosphorylation sites. Dfoxo *F corresponds, in part, to CG3143. The first few exons of CG3143 are *F correct but the 3' end of the gene was misidentified. I intend to submit *F the correct sequence to NCBI within the next few months depending upon *F time constraints. If you wish, I will be happy to contact you when I do. *F yours, *F Brian E. Staveley *F _________________________________________________________________________ *F Dr. Brian E. Staveley, Assistant Professor *F Department of Biology email: bestave@mun.ca *F Memorial University of Newfoundland telephone: 709-737-4317 *F St. John's, Newfoundland A1B 3X9 telefax: 709-737-3018 *F Canada *F http://desmid.biol.mun.ca/brian/BES.html *F _________________________________________________________________________ *F On Thu, 23 Aug 2001, Gillian Millburn wrote: *F > Dear Dr. Staveley, *F > *F > I am curating your abstract for the upcoming European Drosophila *F > conference in Edinburgh, for FlyBase. *F > *F > I am writing in connection with your abstract: *F > *F > H7 'Analysis of foxo in Drosophila melanogaster.' *F > *F > You mention a gene that is new to FlyBase, 'Dfoxo'. *F > *F > Do you know which of the Genome Project CG annotations your gene *F > corresponds to? All the CGs have corresponding gene records in FlyBase *F > already and we don't like to make duplicate records for what is *F > actually the same gene unless we can't avoid it. *F > *F > Any information you give me will be curated as a personal communication *F > from you to FlyBase, *F > *F > Thank you for your help, *F > *F > with best wishes, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- *F > # *U FBrf0138577 *a Roy *b G. *t 2001.8.24 *T personal communication to FlyBase *u *F From: 'Guylaine Roy' *F To: gm119@gen.cam.ac.uk *F Cc: Paul_Lasko@maclan.mcgill.ca *F Subject: Re: Fwd: FlyBase query: EDRC2001: F15 *F Date: Fri, 24 Aug 2001 09:12:56 \-0400 *F Dear Dr. Millburn, *F I answering the e-mail you sent to Dr. Lasko (see below) concerning the dPaip2 *F gene. The CG number for this gene is CG12358. This is the Drosophila homolog *F of the human Paip2 described in Molecular Cell, vol.7, p.205-216, Jan. 2001. *F Thanks a lot for your attention,Guylaine Roy *F McGill University *F Biochemistry Department, room 806 *F 3655 Promenade Sir William Osler *F Montreal, P.Que, Canada, H3G 1Y6 *F tel: (514)398-7275 *F fax: (514)398-1287 *F \----Original Message Follows---- *F Date: 8/23/01 10:02 AM *F Received: 8/24/01 7:53 AM *F From: Gillian Millburn, gm119@gen.cam.ac.uk *F To: Paul_Lasko@maclan.mcgill.ca *F Dear Paul, *F I am curating your abstract for the upcoming European Drosophila *F conference in Edinburgh, for FlyBase. *F I am writing in connection with your abstract: *F F15 'Cloning and characterization of a novel interacting protein for *F the Drosophila poly(A) binding protein, dPaip2' *F You mention a gene that is new to FlyBase, 'dPaip2'. *F Do you know which of the Genome Project CG annotations your gene *F corresponds to? If your gene does not correspond to a CG then could you *F tell me its map location, as this is valuable information for the *F genome annotation project. *F Any information you give me will be curated as a personal communication *F from you to FlyBase, *F with best wishes, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0138578 *a Bocca *b S.N. *t 2001.8.27 *T personal communication to FlyBase *u *F To: pwappner@iib.uba.ar *F Subject: FlyBase query: EDRC2001: F16 *F From: Gillian Millburn (Genetics) *F Date: Thu, 23 Aug 2001 15:03:01 \+0100 *F Dear Dr. Wappner, *F I am curating your abstract for the upcoming European Drosophila *F conference in Edinburgh, for FlyBase. *F I am writing in connection with your abstract: *F F16 'Occurrence of a SCF ubiquitin ligase complex in Drosophila.' *F You mention a gene that is new to FlyBase, 'dRbx1'. *F Do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Any information you give me will be curated as a personal communication *F from you to FlyBase, *F Thank you for your help, *F with best wishes, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From: Silvia Nora Bocca *F To: ''gm119@gen.cam.ac.uk'' *F Subject: EDRC2001:F16 *F Date: Mon, 27 Aug 2001 13:31:05 \-0300 *F Dear Dr Gillian Millburn, *F I am writing on behalf of Dr. Pablo Wappner to give you the information you *F requested concerning a gene that is mentioned in the abstract F16 *F (Occurrence of a SCF ubiquitin ligase complex in Drosophila). *F The gene we termed dRbx1 corresponds to the CG annotation: CG16982, it *F appeared formerly in the Flybase as EG:115C2.11 and recently as Roc1a. *F Please let me know if you need any other information in connection with this *F issue. *F Best wishes, *F Silvia Bocca # *U FBrf0138579 *a Thor *b S. *t 2001.8.26 *T personal communication to FlyBase *u *F To: sthor@HMS.Harvard.edu *F Subject: FlyBase query: EDRC2001: N19 *F From: Gillian Millburn (Genetics) *F Date: Thu, 23 Aug 2001 15:15:27 \+0100 *F Dear Dr. Thor, *F I am curating your abstract for the upcoming European Drosophila *F conference in Edinburgh, for FlyBase. *F I am writing in connection with your abstract: *F N19 'Regulation of FMRFa neuropeptide expression by Apterous and *F Squeeze, a novel Kruppel-type zinc finger transcription factor.' *F You mention a gene that is new to FlyBase, 'squeeze'. *F Do you have a short symbol for this gene. e.g. 'sqz' *F Do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Any information you give me will be curated as a personal communication *F from you to FlyBase, *F Thank you for your help, *F with best wishes, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F Date: Sun, 26 Aug 2001 17:36:23 \-0700 *F To: gm119@gen.cam.ac.uk *F From: Stefan Thor *F Dear Gillian, *F Regarding our abstract: *F N19 'Regulation of FMRFa neuropeptide expression by Apterous and *F Squeeze, a novel Kruppel-type zinc finger transcription factor. *F Additional information: *F gene: squeeze (sqz) *F CG5557 *F l(3)02102 *F 3R 91F4-91F6 *F Was studied in two previous publications but under the gene name l(3)02102 *F Best wishes, *F Stefan Thor Ph.D. *F Assistant Professor of Neurobiology *F Department of Neurobiology, GB 504 *F Harvard Medical School *F 220 Longwood Avenue *F Boston, MA 02115 *F Phone: (617) 432-2351(of) \-2352(lab) *F Fax: (617) 734-7557 *F sthor@HMS.Harvard.edu *F FEDEX acc.no: 1599-9330-2 *F http://neuro.med.harvard.edu/http/stefan/thors.html # *U FBrf0138580 *a Matthews *b B. *t 2001.8.28 *T personal communication to FlyBase *u *F \------------------------------------------------------------------------------ *F -- *F Personal communication from Beverley Matthews, FlyBase-Harvard, Harvard *F University (bmatthew@morgan.harvard.edu) *F Subject: alpha Fucosidase gene *F Dated: 28th August 2001 *F Information communicated: *F Flies heterozygous for Fucn1 (null allele induced by ethyl *F methanesulfonate) and a deficiency for Fuc are viable and fertile. *F Fuc maps within the region deleted by Df(3L)vin2. *F \------------------------------------------------------------------------------ *F -- # *U FBrf0138581 *a Lee *b L. *t 2001.8.28 *T personal communication to FlyBase *u *F To: weaver@wi.mit.edu *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Fri, 24 Aug 2001 16:44:51 \+0100 *F Dear Dr. Orr-Weaver, *F I am curating your paper for FlyBase: *F Lee et al., 2001, Genetics 158(4): 1545--1556 *F In the last paragraph of the Results you say that 'APC5' does not *F affect png. *F You reference Spradling et al., 1999, Genetics 153(1): 135--177 for APC5. *F However, I can't find APC5 in that paper and we have no record of an *F 'APC5' in FlyBase. Could you tell me which P-element 'ACP5' corresponds *F to so I can work out which gene it is, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From: 'Laurie Lee' *F To: gm119@gen.cam.ac.uk *F Date: Tue, 28 Aug 2001 16:26:16 \-0400 *F Subject: FlyBase curation query *F Dear Gillian, *F I'm a postdoc in Terry Orr-Weaver's lab, and she asked me to respond *F to your question about our recent Genetics paper. I obtained the *F information about APC5 from a poster at the 2001 Annual Drosophila *F Research Conference in Washington, D.C.: *F Poster \#145A *F Mutations in ida cause aneuploidy and defects in late cell cycle events. *F A.M. Bentley, B.C. Williams, M.L. Goldberg, A.J. Andres. *F The name 'ida' is short for 'imaginal discs arrested.' The P *F line that I used was l(3)63FbB4. (I obtained the name of the P line *F from the poster.) The Bloomington stock# is 5167. I just looked up *F that stock# on the Bloomington page to double-check my facts, and it is *F now listed as idaB4. The 2001 fly meeting abstract book lists 'ida' *F in the index, but not APC5, so it could have been easily missed. I hope *F that clarifies things. Let me know if you need more information. *F Sincerely, La # *U FBrf0138582 *a Nagel *b A. *t 2001.8.31 *T personal communication to FlyBase *u *F Date: Fri, 31 Aug 2001 11:19:47 \+0200 *F From: Anja Nagel *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query: EDRC2001: J15 *F Dear Gillian, *F the CG annotation is: CG 11525. *F However, let me point out that the gene described by the Genome project is *F not complete according to our data. *F Best regards, Anja *F Dr. Anja Nagel *F Uni Hohenheim *F Institut fuer Genetik (240) *F Garbenstrasse 30 *F 70599 Stuttgart *F Germany *F Fax: \++49/711/4592211 *F Phone: \++49/711/4592215 *F e-mail: anjnagel@uni-hohenheim.de *F 'Gillian Millburn (Genetics)' schrieb: *F > Dear Dr. Nagel, *F > *F > I am curating your abstract for the upcoming European Drosophila *F > conference in Edinburgh, for FlyBase. *F > *F > I am writing in connection with your abstract: *F > *F > J15 'Drosophila Cyclin G interacts directly with H, an antagonist of *F > the Notch signalling pathway' *F > *F > You mention a gene that is new to FlyBase, 'dCycG'. *F > *F > Do you know which of the Genome Project CG annotations your gene *F > corresponds to? All the CGs have corresponding gene records in FlyBase *F > already and we don't like to make duplicate records for what is *F > actually the same gene unless we can't avoid it. If your gene does not *F > correspond to a CG then perhaps you could tell me its map location, as *F > this is valuable information for the genome annotation project. *F > *F > Any information you give me will be curated as a personal communication *F > from you to FlyBase, *F > *F > Thank you for your help, *F > *F > with best wishes, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- # *U FBrf0138583 *a Dunkov *b B. *t 2001.8.25 *T personal communication to FlyBase *u *F To: dunkov@u.arizona.edu *F Subject: FlyBase query: EDRC2001: I14, I15 *F From: Gillian Millburn (Genetics) *F Date: Thu, 23 Aug 2001 15:06:51 \+0100 *F Dear Dr. Dunkov, *F I am curating your abstracts for the upcoming European Drosophila *F conference in Edinburgh, for FlyBase. *F I have questions about 2 abstracts: *F 1. I14 'Diversity and expression of Drosophila melanogaster *F transferrins.' *F You describe 3 transferrin genes in this abstract: Tsf1, Tsf2 and Tsf3. *F Does Tsf1 correspond to the gene currently called 'Transferrin' in *F FlyBase (this gene is also known as CG6186) ? *F Do you know which of the Genome Project CG annotations Tsf2 and Tsf3 *F correspond to? *F If Tsf2 and Tsf3 do not correspond to a CG then perhaps you could tell *F me their map location, as this is valuable information for the genome *F annotation project. *F 2. I15 'Probing insect heme catabolism: characterization of Drosophila *F melanogaster heme oxygenase and biliverdin reductase.' *F This abstract describes 2 genes that are new to FlyBase: HO (heme *F oxygenase) and BVR (biliverdin IX-beta reductase). *F Again could you tell me which CG gene these 2 genes correspond to, or *F their map location. *F Any information you give me will be curated as a personal communication *F from you to FlyBase, *F Thank you for your help, *F with best wishes, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F Date: Fri, 24 Aug 2001 18:47:51 \-0700 *F To: Gillian Millburn (Genetics) *F From: Boris Dunkov *F Subject: Re: FlyBase query: EDRC2001: I14, I15 *F Dear Gillian, *F Here are the answers to your questions: *F >Does Tsf1 correspond to the gene currently called 'Transferrin' in *F >FlyBase (this gene is also known as CG6186) ? *F Yes, it does. We chose 'Tsf' for a gene symbol, since 'Tf' and 'Trf' are *F already taken. *F > *F >Do you know which of the Genome Project CG annotations Tsf2 and Tsf3 *F >correspond to? *F Tsf2 = CG10620 *F Tsf3 = CG3666 *F >This abstract describes 2 genes that are new to FlyBase: HO (heme *F >oxygenase) and BVR (biliverdin IX-beta reductase). *F HO = CG14716 *F BVR = CG9471 *F there is also a biliverdin IX alpha reductase gene = CG3597 (the gene *F product is not yet described in the literature, but it probably would be *F named 'BVRA').. *F I hope this answers your questions.. *F Best wishes, *F Boris Dunkov *F Department of Biochemistry and Molecular Biophysics *F University of Arizona *F BSW \# 351 *F Tucson, AZ 85719 *F Tel.(520)621-3046 *F FAX (520)621-9288 *F dunkov@u.arizona.edu # *U FBrf0138585 *a Overton *b P. *t 2001.9.6 *T personal communication to FlyBase *u *F Date: Thu, 6 Sep 2001 16:32:34 \+0100 *F From: Paul Overton *F To: Gillian Millburn *F Subject: Re: FlyBase query: EDRC2001: M16 *F Hi Gillian, *F so Sox21a is CG7345 *F Sox21b is CG6419 and CG13483, which is actually the 3' exon (transcript *F structure looks a bit different to GeneScene). *F Sox102F is the same as Sox102D; it's the second last gene so it's got to *F be in F.. This one is CG11153. *F Cheers, *F P *F \------------------------------------------------------------------------------- *F Paul Overton pmo22@mole.bio.cam.ac.uk *F Department of Genetics *F University of Cambridge Phone: (+44) 01223 766488 *F Downing Street Fax: (+44) 01223 333992 *F Cambridge *F CB2 3EH http://www.gen.cam.ac.uk/dept/russell.html *F \------------------------------------------------------------------------------- *F On Thu, 6 Sep 2001, Gillian Millburn wrote: *F > Hi Paul, *F > *F > here is the e-mail I sent Steve. .. *F > *F > Gillian *F > *F > \----- Begin Included Message \----- *F > *F > >From gm119@gen.cam.ac.uk Thu Aug 23 15:11:18 2001 *F > Envelope-to: gm119@gen.cam.ac.uk *F > Delivery-date: Thu, 23 Aug 2001 15:11:18 \+0100 *F > To: SR120@mole.bio.cam.ac.uk *F > Subject: FlyBase query: EDRC2001: M16 *F > Cc: gm119@gen.cam.ac.uk *F > X-Sun-Charset: US-ASCII *F > From: Gillian Millburn (Genetics) *F > Date: Thu, 23 Aug 2001 15:11:20 \+0100 *F > Content-Length: 1118 *F > *F > Hi Steve, *F > *F > I am curating your abstract for the upcoming EDRC. *F > *F > In your abstract: *F > *F > M16 'Two Drosophila Group B Sox genes, 21a and 21b and a Group D Sox *F > gene, 102F are expressed dynamically during embryonic development.' *F > *F > you mention 3 Sox genes: Sox21a, Sox21b, Sox102F *F > *F > Can you tell me which CGs they correspond to so I can rename the CGs. *F > *F > *F > .. *F > *F > ta *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- *F > *F > *F > \----- End Included Message \----- *F > *F > # *U FBrf0138586 *a O'Farrell *b P.H. *t 2001.9.11 *T personal communication to FlyBase *u FlyBase error report for CG13852 on Mon Sep 10 18:52:22 2001. *F From FlyBase-error@hedgehog.lbl.gov Tue Sep 11 02:52:24 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 11 Sep 2001 02:52:24 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 10 Sep 2001 18:52:22 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: ofarrell@cgl.ucsf.edu *F Subject: FlyBase error report for CG13852 on Mon Sep 10 18:52:22 2001 *F Content-Length: 525 *F Error report from Patrick H. O'Farrell (ofarrell@cgl.ucsf.edu) *F Gene or accession: CG13852 *F Release: 1 *F Missed gene *F Comments: The sequence is the best Drosophila homolog to S. cerevisiae cell *F cycle regulator MOB1 (2xe-48 from psi blast) and to the analogous gene of S. *F pombe Sid2 (6xe-61). *F Flybase lists other genes as homologous to Mob1 \- the second best homolog is *F CG4946 which scores 7xe-40 against MOB1 and 3xe-48 against Sid2. *F Browser: Mozilla/4.7 (Macintosh; I; PPC) *F Accessed from: query.pl, /www/hedgehog_8001/htdocs # *U FBrf0138587 *a Whitfield *b E. *t 2001.9.10 *T personal communication to FlyBase *u *F Date: Mon, 10 Sep 2001 16:54:56 \+0100 *F From: Eleanor Whitfield *F To: Gillian Millburn *F Subject: mth error report *F Hi, *F .. *F mth: *F AE003468; AAF47379 *F annotation of 1 splice site is a little wrong such that the translation *F given by Celera is missing 1 amino acid (position 281, when comparing to *F AF109308; AAD16981, Lin et al, Science 282:943-946(1998)) *F CG17795, mth-like 2 *F AE003565; AAF50761 *F this translation is 482aa, Fig 3 of Brody T., Cravchik A., J. Cell Biol. *F 150:F83-F88(2000) show an extra C-terminal exon that translates: *F IFPKQRAF SRSATQSTIE SISQTKRHFN MT *F (80086-88 to 80173-175, exact positions I did not determine) which now *F translates a 512 aa protein. *F CG6530, mth-like 3 *F original 192aa protein (AE003803; AAF57863) has been replaced by 458aa *F translation: *F MRIVIGSFTA FLLLLLQNSN AEIPGCDFFD TVDISKAPRF SNGSYLYEGL LIPAHLTAEY *F DYKLLADDSK EKVASHVRGC ACHLRPCIRF CCPQYQKMQK SKCYGDMSED ELNKHDPFVN *F VTLSDGSVVR RHFKEDLIVQ SDLAKPGCPR MYFLNHELPM SIFTAIVISL ICIILTISVY *F LYVEKLRNLH GKCFICYLAS LFLGYFFLVL NVWKYSSGFC VTAGFLGYFS VIAAFFWLSV *F ISLTLWNSFS GNSSWLNRFL PQNRFLSYNL YAWGMALLLT AITYIADQVV KNEKLRPRVG *F VGKNCWIYTG DMTVMIYFYG PMLLIIVFNI TMFVLTAFRI MKVKKEAQNF TQQQKTTNRL *F NSDKQTYALF LRLFIIMGLS WSLEIISFLL SKNQAWAKAF MVADYFNWSQ GTVIFLLFVL *F RPSTLKLLKE RIKGGRDEAG ASDEHISLQN TKIDPSVF *F in same Fig as above (cannot remember how many exons were added, 3 or *F 4). *F CG6536, mth-like 4 *F original 362 aa protein (AE003803; AAF57864) has an additional 12 aa at *F the N-terminus and revised C-terminus so the translation is now 468aa: *F MPKSNAEIPG CDFFDTVDIS KAPRFSNGSY LYEGLLIPAH LTAEYDYKLL ADDSKEKVAS *F HVRGCACHLR PCIRFCCPQY QKMQKSKCYG DMSEDELNKH DPFVNVTLSD GSVVRRHFKE *F DLIVQSDLAK PGCPRMYFLN HELPGNEFTL FENGSLLRHW DKVELSKREY CVQHLSFKDD *F SIRIAPHFCP LSSEHSRTWK TVAIVISLIC IILTISVYLY VEKLRNLHGK CFICYLASLF *F LGYFFLVLNV WKYSSGFCVT AGFLGYFSVM AAFFWLSVIG IHLRIKFSLA SNCLHRLLPE *F NPFRAYNLYA WGIPLIMTAI TYTADQVVKN EKLRPRVGVG KNCWIYTGDM TVMIYFYGPM *F LLLIAFNIIM FVLSAIYIYN IKKNVKGLVH KQQTNQQIND QQMFAIFLRL FILMGLSWSF *F EILSFLLTKQ QAWARALMVA DYFNWSQGTI IFVLFILKPS ILKLIIAG *F from same figure again (additional 2 exons). *F CG16992, mth-like 6 *F current 172aa translation (AE003558; AAF50551) fails to encode any of *F the 7 transmembrane required for a GPCR. *F CG7476, mth-like 7 *F 426aa translation (AE003556; AAF50475) has 5 of the 7 transmembrane *F regions required for a GPCR, 2 C-terminal domains are missing. # *U FBrf0138588 *a Green *b R. *c T. *d Brody *e J. *f Lengyel *t 2001.8.18 *T personal communication to FlyBase *u FlyBase error report for CG10016 on Fri Aug 17 16:31:04 2001. *F From FlyBase-error@hedgehog.lbl.gov Sat Aug 18 00:31:07 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Sat, 18 Aug 2001 00:31:07 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 17 Aug 2001 16:31:04 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: rbgreen@ucla.edu *F Subject: FlyBase error report for CG10016 on Fri Aug 17 16:31:04 2001 *F Content-Length: 1132 *F Error report from Ryan Green, Tom Brody, Judith Lengyel (rbgreen@ucla.edu) *F Gene or accession: CG10016 *F Release: 1 *F Gene annotation error *F Gene CG10016 has incorrect exon/intron structure or translation start site. *F Protein sequence: *F >Drm *F M F A V M R I D N D D C R S D F R R K M R P K C E F *F I C K Y C Q R R F T K P Y N L M I H E R T H K S P E *F I T Y S C E V C G K Y F K Q R D N L R Q H R C S Q C *F V W R *F Comments: We have sequenced the complete cDNA for the drumstick (drm) gene *F (CG10016) and have found the ORF encodes a protein that is 81 amino acids in *F length. Sequence data from EST clones (LD26791, GH27717, and LD15344), RT-PCR *F products, and 5' & 3' RACE products, all provide similar supporting evidence. *F An intron occurs after the second nucleotide of the R74 codon. The intron is *F 923 bp in length. The following exon begins with the third nucleotide of the *F R74 codon and is followed by seven residues (CSQCVWR) before the stop codon. # *U FBrf0138590 *a Locke *b J. *c S. *d Maier *t 2001.9.16 *T personal communication to FlyBase *u *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 16 Sep 2001 20:48:17 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: john.locke@ualberta.ca *F Subject: FlyBase error report for CG9621 on Sun Sep 16 20:48:17 2001 *F Error report from John Locke / Stephanie Maier (john.locke@ualberta.ca) *F Gene or accession: CG9621 *F Release: 1 *F Gene annotation error *F Gene CG9621 has incorrect exon/intron structure or translation start site. *F Protein sequence: *F MAMWFLGALVATVVLLTGSVQATDLPYETLREQIMEAERVASLGGNIWLSSDEEKANSILMNAKRAEIAEGLKTPEKYA *F PAMHFFQGRQYVRQSEVFRMIQKIPKGAFLHGHNTGMVTSRWIIQNLTTTNNLYTCRNVDGLLVFTYDQSGCHSEVQNV *F CTERINAEDRGKYERQLEKHINMHGPRPEALLPNRKKIWERFENIFTTVDRLYKYRPTYCAYHKRMLEELCEDNIIYAE *F IRASLSPLYDDNNRTLSTLEVANELERIVEEFKAKHHDFIGVKVIYAKRNRASEEEMLRRITTFKQLHHAKPNFVIGFD *F LIGQEDTGEPLNRYINQLSDLPSTANYFFHAGETNWNGRTDWNMMDAILLNTKRIGHAFALPKHPQLWSTIKKRNIAIE *F VNPISNQVLGFVWDLRNHPASFLIAENFPIVISSDDPGVWGAKGLSYDFYYAFMALAPADADLRFLKQLALNSIKYAVL *F TSDERRKINRVFQRKWQEFIANVLNPKF *F Comments: The protein sequence (AAF54979) is missing the amino acids *F corresponding to exon 2 of the gene. The gene sequence is correct as *F presented in Gadfly. # *U FBrf0138591 *a Misra *b S. *t 2001.8.10 *T personal communication to FlyBase *u *F Date: Fri, 10 Aug 2001 15:48:28 \-0700 (PDT) *F From: Sima Misra *F To: Gillian Millburn *F Subject: Re: New merges *F .. *F I've attached a file with the 18 cDNAs that indicate gene merges should *F take place. 2 of the 18 are possible chimeric clones, and should probably *F not be merged. A few of the cases are merges of 3 genes. I gather you *F have already merged some of these. Let me know if something looks wrong. *F For all but one of these I took the full-length cDNA sequence, did blastn *F searches against na_gadfly.dros.RELEASE1 and na_geno.dros.RELEASE1 at *F FlyBLAST. In one case, I did a local blastn against *F na_gadfly.dros.RELEASE2 and na_geno.dros.RELEASE2. *F Cheers, *F Sima *F \------------------------------------------------------------------------------ *F -- *F LD27988=CG3101 *F [By BLAST 908-2649 of the 3045bp BcDNA:LD27988 matches CG3101 and *F is on AE003447.1, 1-916 of LD27988 matches CG3102 and is on AE003448.1; this *F says CG3101 and CG3102 should be merged.] *F SD05444=Tim9b *F [By BLAST 606-1484 of the 1582bp BcDNA:SD05444 matches CG12788 and is *F on AE003512.1, 1-508 of SD05444 matches Tim9b(CG17767) and is on AE003512.1; *F this says Tim9b(CG17767) and CG12788 should be merged.] *F GH24627=CG8572 *F [By BLAST 897-2090 of the 2652bp BcDNA:GH24627 matches CG8576 and *F is on AE003559.1, 1-901 matches CG8572 and is on AE003559.1; this says that *F CG8572 and CG8576 should be merged.] *F GH20492=CG18020 *F [By BLAST 1941-3823 of the 3974bp BcDNA:GH20492 matches CG18021 and *F is on AE003462.1, 1-1794 matches CG18020 and is on AE003462.1; this says that *F CG18020 and CG18021 should be merged.] *F GH21518=CG4000? \!Possible chimeric clone! *F [By BLAST 562-1539 of the 1557bp BcDNA:GH21518 matches CG4000 and *F is on AE003731.1, 1-380 matches CG13841 and is on AE003740.1; this says that *F CG4000 and CG13841 should perhaps be merged. However, because these are so *F far apart in the genome (92E8-92F5 versus 94C1-94D3), this may be a case of a *F chimeric clone rather than a merged gene]. *F LD29969=CG18787 *F [By BLAST 186-1070 of the 1386bp cDNA:LD29969 matches CG18789 and *F is on AE003634.2, 1-982 matches CG18787 and is on AE003634.2; this says that *F CG18787 and CG18789 should be merged.] *F GH03013=CG3550 *F [By BLAST 1-988 of the 2553bp BcDNA:GH03013 matches CG3550 and is *F on AE003458.1, 987-2219 matches CG11605 and is on AE003458.1; this says that *F CG3550 and CG11605 should be merged.] *F LP01670=Lcp1? \!Possible chimeric clone! *F [By BLAST 582-1507 of the 2080bp BcDNA:LP01670 matches CG6044 and is *F on AE003457.1, 1-532 matches Lcp1(CG11650) and is on AE003837.1; this says that *F CG6044 and Lcp1 should perhaps be merged. However, because these are so *F far apart in the genome (58D2-58E8 versus 44B8-44C6), this may be a case of a *F chimeric clone rather than a merged gene]. *F LD47466=dlp *F [By BLAST 820-3093 of the 3762bp BcDNA:LD47466 matches dlp(CG5031) *F and is on AE003533.1, 1-766 matches CG17703 and is on AE003534.1; this says *F that dlp(CG5031) and CG17703 should be merged.] *F LD40565=CG7102 *F [By BLAST 887-1985 of 2089bp BcDNA:LD40565 matches CG7102 and is on *F AE003619.1, and 1-890 matches CG17973 and is on AE003619.1; this says that *F CG7102 and CG17973 should be merged.] *F GH11472=CG5544 *F [By BLAST 834-2268 of the 2289bp BcDNA:GH11472 matches CG5544 and is *F on AE003799.1, and 1-799 matches CG16859 and is on AE003799.1; this says that *F CG5544 and CG16859 should be merged.] *F LD39166=Hs6st *F [By BLAST 725-1825 of the 2656bp BcDNA:LD39166 matches CG4451 and is *F on AE003728.1, 1-754 matches Hs6st(CG18533) and is on AE003728.1, 1821-1957 *F matches CG17188 and is on AE003728.1; this says that Hs6st and CG4451 and *F CG17188 should be merged.] *F SD07650=CG9510 *F [By BLAST 1232-2772 of the 2797bp BcDNA:SD07650 matches CG9510 and *F is on AE003623.1, 1-809 matches CG9515 and is on AE003623.1; this says that *F CG9510 and CG9515 should be merged.] *F GH15471=Myosin-heavy-chain-like *F [By BLAST 1183-6127 of the 6223bp BcDNA:GH15471 matches *F Myosin-heavy-chain-like(CG10218) and is on AE003711.1, 1-1094 matches CG10224 *F and is on AE003711.1; this says that Myosin-heavy-chain-like(CG10218) and *F CG10224 should be merged.] *F GH13229=CG9584 *F [By BLAST 976-2691 of the 2764bp BcDNA:GH13229 matches CG9585 and *F is on AE003623.1, 1-780 matches CG9584 and is on AE003623.1, 762-986 matches *F CG18566 and is on AE003623.1; this says that CG9584 and CG9585 and CG18566 *F should be merged.] *F LD47625=CG14954 *F [By BLAST 641-1528 of the 2347bp BcDNA:LD47625 matches CG16745 and *F is on AE003476.1, 274-651 matches CG14954 and is on AE003477.1; this says that *F CG14954 and CG16745 should be merged.] *F SD03168=CG10439 *F [By BLAST 816-2753 of the 3307bp BcDNA:SD03168 matches CG13435 *F and is on AE003791.1, 1-831 matches CG10439 and is on AE003791.1; this says *F that CG10439 and CG13435 should be merged.] *F LD29569=CG12790 *F [By BLAST 1665-3028 of the 3601bp cDNA:LD29569 matches CG12790 and *F is on AE003475.1, 1-1106 matches CG13797 and is on AE003474.1, *F 1093-1668 matches CG16763 and is on AE003475.1; this says that CG12790 *F and CG13797 and CG16763 should be merged.] *F \------------------------------------------------------------------------------ *F -- # *U FBrf0138592 *a Peifer *b M. *t 2001.8.28 *T personal communication to FlyBase *u FlyBase error report for CG17484 on Tue Aug 28 08:26:34 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 28 Aug 2001 08:26:35 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG17484 on Tue Aug 28 08:26:34 2001 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG17484 *F Release: 1 *F cDNA or EST error *F Gene cDNA sequence: *F >p120ctn *F 1 tcggcacgag gttgtactaa atgctttaat atctggaaaa tcggatttca attttttgtg *F 61 aatatgtagt tttacatatg gacatctatg gaagcgcgat ctctcaaaca cagcctaatc *F 121 ggtttaaata tgaattcaac tcacataaac atggaccttt cattgacgca cgagcctgca *F 181 aacatgcaat cccaatttgg gtcttttcaa aattatgatc aattttcggc agcaggatat *F 241 tcaagcactc cattatatat aacaaagccc acaggagtag gtgcaataac aaaagttgcg *F 301 ccacactgct ctcaaaataa gattgagacg aactctacag acaattccat tccagacatt *F 361 gagaatcatc cactagctac aacagtgaga tatgtacaag cgggtcaata tgaggataca *F 421 tccgaatatg gtgtagctgg tttgacttgt ggaatagact ctgagtacac agctgaagct *F 481 gtcgtacctt actgctatac gtcggatgca aactatacag gaattcaaaa cacgacttca *F 541 agtattaaac cattttctgg aaggcctttt aatgataaca taaatggagc agaagctgtt *F 601 gcggattccc aatgtagaac ctttaaaagt tcagctgaat ttaaattacc tcaattcgcc *F 661 atgagctcta taaatacagt gccgcaaaga ttggaagaaa aggatgatta cattgaagga *F 721 tcagaaaatg atatttgttc tacaatgaga tggcgggatc caaatctttc agaagtaata *F 781 agctttttaa gcaacccgag tagtgctata aaagctaatg cagcagccta tctacagcat *F 841 ttgtgctaca tggatgatcc aaacaagcaa cgtacaagat ctctgggagg tataccacca *F 901 ttagttcgac tgctatctta tgattctcca gaaatacata aaaatgcctg tggtgcttta *F 961 cgtaatttat cttacggaag acaaaatgat gaaaacaagc gtggaataaa gaacgctggt *F 1021 ggaatagcag ctttggtgca tcttttatgt cggtcacaag aaacagaagt aaaagaattg *F 1081 gtgactggcg ttttgtggaa tatgtcttcc tgtgaagact tgaaacggtc aattattgat *F 1141 gaagcgctcg ttgctatagt ttgcagcgtg attaagcctc attctggttg ggatgcagtt *F 1201 tgttgtggag aaacatgctt ttctacggta tttcgaaatg cttctggagt tttacgaaat *F 1261 gtcagttctg ctggagaaca tgcaagagga tgtcttcgaa attgtgaaca tttagttgaa *F 1321 tgccttcttt atgttgtgcg aacatcaatt gagaaaaata atattggaaa taaaactgta *F 1381 gaaaattgcg tatgtatact tcgaaatctt tcataccgat gtcaagaagt tgatgatccc *F 1441 aattacgaca aacatccctt tataacacca gaaagagtta ttccatcgtc atctaaagga *F 1501 gaaaatttgg gctgttttgg aacaaataaa aaaaaaaaag aagctaataa ttccgatgct *F 1561 ctcaacgaat acaacatttc cgctgactat tctaaaagtt ctgtaacata taacaaatta *F 1621 aacaagggat atgaacaatt atggcaacct gaagtagttc aatactattt gtcattattg *F 1681 caaagttgtt ctaatccaga aacacttgaa gccgctgctg gagcaattca aaatttgtct *F 1741 gcatgctatt ggcaacccag tattgatatt cgcgcaacag tgcgtaaaga gaaaggccta *F 1801 ccgatacttg ttgagcttct taggatggaa gtagatcgcg tagtttgtgc agtcgccaca *F 1861 gcactacgca atttagctat agatcaacgc aacaaagaac taattggaaa gtacgcaatg *F 1921 cgtgacttag ttcagaaact tccatccgga aatgtacaac atgaccaaaa tacatcagat *F 1981 gatacaatca cagctgtatt agcaacaatt aatgaggtta taaaaaaaaa tccagagttt *F 2041 tcccgttccc tattggattc aggtgggata gatagactta tgaacataac aaaacggaaa *F 2101 gaaaaatata cctcttgtgt gttgaaattt gcaagtcaag ttttatacac catgtggcaa *F 2161 cataatgaat tgcgagacgt ttataaaaaa aatggatgga aagagcaaga ttttgttagt *F 2221 aaacatttta ctgcacataa tactccacca agttcaccga ataacgttaa caatacactt *F 2281 aatagaccga tggcttcaca gggacgtact cgctatgagg acagaaccat tcaacgcgga *F 2341 acaagcacat tatatagtgc taacgattcc agtggtgccg ttatgtctaa tgaatctgca *F 2401 atgctttcag aaatggttag aaaacaacta taaaattggt tgttttttta ttattaaagt *F 2461 atttaaaaat acataaatga attcaaagtt taaattatat taacttatat tccaaatagc *F 2521 aaagagggat ttgctatatt tactcaactt aattgtaaaa tatcatatta aaaaatattt *F 2581 agagcactaa tcagataatt taataaataa aataagaata tcaaagtttt aataaaaaaa *F Protein sequence: *F >p120ctn *F MEARSLKHSLIGLNMNSTHINMDLSLTHEPANMQSQFGSFQNYD *F QFSAAGYSSTPLYITKPTGVGAITKVAPHCSQNKIETNSTDNSIPDIENHPLATTVRY *F VQAGQYEDTSEYGVAGLTCGIDSEYTAEAVVPYCYTSDANYTGIQNTTSSIKPFSGRP *F FNDNINGAEAVADSQCRTFKSSAEFKLPQFAMSSINTVPQRLEEKDDYIEGSENDICS *F TMRWRDPNLSEVISFLSNPSSAIKANAAAYLQHLCYMDDPNKQRTRSLGGIPPLVRLL *F SYDSPEIHKNACGALRNLSYGRQNDENKRGIKNAGGIAALVHLLCRSQETEVKELVTG *F VLWNMSSCEDLKRSIIDEALVAIVCSVIKPHSGWDAVCCGETCFSTVFRNASGVLRNV *F SSAGEHARGCLRNCEHLVECLLYVVRTSIEKNNIGNKTVENCVCILRNLSYRCQEVDD *F PNYDKHPFITPERVIPSSSKGENLGCFGTNKKKKEANNSDALNEYNISADYSKSSVTY *F NKLNKGYEQLWQPEVVQYYLSLLQSCSNPETLEAAAGAIQNLSACYWQPSIDIRATVR *F KEKGLPILVELLRMEVDRVVCAVATALRNLAIDQRNKELIGKYAMRDLVQKLPSGNVQ *F HDQNTSDDTITAVLATINEVIKKNPEFSRSLLDSGGIDRLMNITKRKEKYTSCVLKFA *F SQVLYTMWQHNELRDVYKKNGWKEQDFVSKHFTAHNTPPSSPNNVNNTLNRPMASQGR *F TRYEDRTIQRGTSTLYSANDSSGAVMSNESAMLSEMVRKQL *F Comments: To whom it may concern, *F I would like to update the Gadfly and Geneseen entries for p120ctn (CG17484) *F The splicing pattern predicted by the your prediction programs and displayed *F on the database does not match the splicing pattern of the full length cDNA *F which we sequenced, nor does it fit with additional ests which are in the BDGP *F database. Our p120 cDNA sequence, along with our predicted protein sequence, *F are deposited in Genbank (AF220496-pasted below). The differences are as *F follows: *F 1) Exon 2 differs in its 5’ splice junction. As a result, it alters the *F reading frame and means that the BDGP predicted protein starts at our amino *F acid 33. *F 2) There are differences in 3’ splice junction of exon 3 and the 5’ splice *F junction of exon 4, that mean the the BDGP predicted protein lacks a number of *F amino acids present in p120. *F 3) Finally, our sequence predicts a gene with only four exons, not five. Our *F cDNA and the BDGP EST LD03740 continue past the putative 3’splice junction of *F the BDGP exon 4 and reach a consensus poly-A addition site and a poly A tail. *F The current BDGP exon 5 has no matches in the EST collection, nor does it *F match anything else in flies except the CG to which it is assigned and the *F genomic sequences from which it is derived. We think it is unlikely that exon *F 5 exists. *F If you have any questions, I’d be happy to discuss this further. *F Mark Peifer *F LOCUS AF220496 2640 bp mRNA INV 11-FEB-2000 *F DEFINITION Drosophila melanogaster arm repeat protein (p120ctn) mRNA, complete *F cds. *F ACCESSION AF220496 *F VERSION AF220496.1 GI:6959879 *F KEYWORDS . *F SOURCE fruit fly. *F ORGANISM Drosophila melanogaster *F Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; *F Muscomorpha; Ephydroidea; Drosophilidae; Drosophila. *F REFERENCE 1 (bases 1 to 2640) *F AUTHORS Cavallo,R., Myster,S. and Peifer,M. *F TITLE A Drosophila melanogaster homolog of the adherens junction protein *F p120ctn *F JOURNAL Unpublished *F REFERENCE 2 (bases 1 to 2640) *F AUTHORS Cavallo,R., Myster,S. and Peifer,M. *F TITLE Direct Submission *F JOURNAL Submitted (03-JAN-2000) Biology, University of North Carolina, *F South Road, CB 3280, Chapel Hill, NC 27599-3280, USA *F FEATURES Location/Qualifiers *F source 1..2640 *F /organism='Drosophila melanogaster' *F /db_xref='taxon:7227' *F /chromosome='2' *F /map='2R; 41A-C' *F gene 1..2640 *F /gene='p120ctn' *F CDS 88..2433 *F /gene='p120ctn' *F /codon_start=1 *F /product='arm repeat protein' *F /protein_id='AAF33245.1' *F /db_xref='GI:6959880' *F /translation='MEARSLKHSLIGLNMNSTHINMDLSLTHEPANMQSQFGSFQNYD *F QFSAAGYSSTPLYITKPTGVGAITKVAPHCSQNKIETNSTDNSIPDIENHPLATTVRY *F VQAGQYEDTSEYGVAGLTCGIDSEYTAEAVVPYCYTSDANYTGIQNTTSSIKPFSGRP *F FNDNINGAEAVADSQCRTFKSSAEFKLPQFAMSSINTVPQRLEEKDDYIEGSENDICS *F TMRWRDPNLSEVISFLSNPSSAIKANAAAYLQHLCYMDDPNKQRTRSLGGIPPLVRLL *F SYDSPEIHKNACGALRNLSYGRQNDENKRGIKNAGGIAALVHLLCRSQETEVKELVTG *F VLWNMSSCEDLKRSIIDEALVAIVCSVIKPHSGWDAVCCGETCFSTVFRNASGVLRNV *F SSAGEHARGCLRNCEHLVECLLYVVRTSIEKNNIGNKTVENCVCILRNLSYRCQEVDD *F PNYDKHPFITPERVIPSSSKGENLGCFGTNKKKKEANNSDALNEYNISADYSKSSVTY *F NKLNKGYEQLWQPEVVQYYLSLLQSCSNPETLEAAAGAIQNLSACYWQPSIDIRATVR *F KEKGLPILVELLRMEVDRVVCAVATALRNLAIDQRNKELIGKYAMRDLVQKLPSGNVQ *F HDQNTSDDTITAVLATINEVIKKNPEFSRSLLDSGGIDRLMNITKRKEKYTSCVLKFA *F SQVLYTMWQHNELRDVYKKNGWKEQDFVSKHFTAHNTPPSSPNNVNNTLNRPMASQGR *F TRYEDRTIQRGTSTLYSANDSSGAVMSNESAMLSEMVRKQL' *F BASE COUNT 932 a 456 c 497 g 755 t *F ORIGIN *F 1 tcggcacgag gttgtactaa atgctttaat atctggaaaa tcggatttca attttttgtg *F 61 aatatgtagt tttacatatg gacatctatg gaagcgcgat ctctcaaaca cagcctaatc *F 121 ggtttaaata tgaattcaac tcacataaac atggaccttt cattgacgca cgagcctgca *F 181 aacatgcaat cccaatttgg gtcttttcaa aattatgatc aattttcggc agcaggatat *F 241 tcaagcactc cattatatat aacaaagccc acaggagtag gtgcaataac aaaagttgcg *F 301 ccacactgct ctcaaaataa gattgagacg aactctacag acaattccat tccagacatt *F 361 gagaatcatc cactagctac aacagtgaga tatgtacaag cgggtcaata tgaggataca *F 421 tccgaatatg gtgtagctgg tttgacttgt ggaatagact ctgagtacac agctgaagct *F 481 gtcgtacctt actgctatac gtcggatgca aactatacag gaattcaaaa cacgacttca *F 541 agtattaaac cattttctgg aaggcctttt aatgataaca taaatggagc agaagctgtt *F 601 gcggattccc aatgtagaac ctttaaaagt tcagctgaat ttaaattacc tcaattcgcc *F 661 atgagctcta taaatacagt gccgcaaaga ttggaagaaa aggatgatta cattgaagga *F 721 tcagaaaatg atatttgttc tacaatgaga tggcgggatc caaatctttc agaagtaata *F 781 agctttttaa gcaacccgag tagtgctata aaagctaatg cagcagccta tctacagcat *F 841 ttgtgctaca tggatgatcc aaacaagcaa cgtacaagat ctctgggagg tataccacca *F 901 ttagttcgac tgctatctta tgattctcca gaaatacata aaaatgcctg tggtgcttta *F 961 cgtaatttat cttacggaag acaaaatgat gaaaacaagc gtggaataaa gaacgctggt *F 1021 ggaatagcag ctttggtgca tcttttatgt cggtcacaag aaacagaagt aaaagaattg *F 1081 gtgactggcg ttttgtggaa tatgtcttcc tgtgaagact tgaaacggtc aattattgat *F 1141 gaagcgctcg ttgctatagt ttgcagcgtg attaagcctc attctggttg ggatgcagtt *F 1201 tgttgtggag aaacatgctt ttctacggta tttcgaaatg cttctggagt tttacgaaat *F 1261 gtcagttctg ctggagaaca tgcaagagga tgtcttcgaa attgtgaaca tttagttgaa *F 1321 tgccttcttt atgttgtgcg aacatcaatt gagaaaaata atattggaaa taaaactgta *F 1381 gaaaattgcg tatgtatact tcgaaatctt tcataccgat gtcaagaagt tgatgatccc *F 1441 aattacgaca aacatccctt tataacacca gaaagagtta ttccatcgtc atctaaagga *F 1501 gaaaatttgg gctgttttgg aacaaataaa aaaaaaaaag aagctaataa ttccgatgct *F 1561 ctcaacgaat acaacatttc cgctgactat tctaaaagtt ctgtaacata taacaaatta *F 1621 aacaagggat atgaacaatt atggcaacct gaagtagttc aatactattt gtcattattg *F 1681 caaagttgtt ctaatccaga aacacttgaa gccgctgctg gagcaattca aaatttgtct *F 1741 gcatgctatt ggcaacccag tattgatatt cgcgcaacag tgcgtaaaga gaaaggccta *F 1801 ccgatacttg ttgagcttct taggatggaa gtagatcgcg tagtttgtgc agtcgccaca *F 1861 gcactacgca atttagctat agatcaacgc aacaaagaac taattggaaa gtacgcaatg *F 1921 cgtgacttag ttcagaaact tccatccgga aatgtacaac atgaccaaaa tacatcagat *F 1981 gatacaatca cagctgtatt agcaacaatt aatgaggtta taaaaaaaaa tccagagttt *F 2041 tcccgttccc tattggattc aggtgggata gatagactta tgaacataac aaaacggaaa *F 2101 gaaaaatata cctcttgtgt gttgaaattt gcaagtcaag ttttatacac catgtggcaa *F 2161 cataatgaat tgcgagacgt ttataaaaaa aatggatgga aagagcaaga ttttgttagt *F 2221 aaacatttta ctgcacataa tactccacca agttcaccga ataacgttaa caatacactt *F 2281 aatagaccga tggcttcaca gggacgtact cgctatgagg acagaaccat tcaacgcgga *F 2341 acaagcacat tatatagtgc taacgattcc agtggtgccg ttatgtctaa tgaatctgca *F 2401 atgctttcag aaatggttag aaaacaacta taaaattggt tgttttttta ttattaaagt *F 2461 atttaaaaat acataaatga attcaaagtt taaattatat taacttatat tccaaatagc *F 2521 aaagagggat ttgctatatt tactcaactt aattgtaaaa tatcatatta aaaaatattt *F 2581 agagcactaa tcagataatt taataaataa aataagaata tcaaagtttt aataaaaaaa # *U FBrf0138593 *a Bayraktaroglu *b L. *t 2001.8.23 *T personal communication to FlyBase *u FlyBase error report for CG14513 on Thu Aug 23 07:51:58 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 23 Aug 2001 07:51:58 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: leyla@morgan.harvard.edu *F Subject: FlyBase error report for CG14513 on Thu Aug 23 07:51:58 2001 *F Error report from Leyla Bayraktaroglu (leyla@morgan.harvard.edu) *F Gene or accession: CG14513 *F Release: 1 *F Gene annotation error *F Genes CG14513 and CG11879 should be merged. *F Comments: The 228 bp CG14513 'transcript' is part of the 3'UTR of yemalpha *F (FBgn0005596). *F Nucleotides 1..228 of CG14513 align with nucleotides 4777..5004 *F of GenBank DNA accession X63503 for yemalpha. The 6th and final exon *F of yemalpha is encoded by nucleotides 3372..5259 of accession X63503. *F Here is the BLAST alignment of the cDNA FASTA of CG14513 with GenBank *F accession X63503: *F >gi|8837|emb|X63503.1|DMYEMA D.melanogaster gene for yemanuclein-alpha *F Length = 5696 *F Score = 452 bits (228), Expect = e-125 *F Identities = 228/228 (100%) *F Strand = Plus / Plus *F Query: 1 atggccgccgccatcgtgctctccacgatgagccacgcctcgccgcgctacgacctgctg 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4777 atggccgccgccatcgtgctctccacgatgagccacgcctcgccgcgctacgacctgctg 4836 *F Query: 61 gccaacggctccgtctccatgtccgccgcccaggtgggagcctctgccaacacactgcca 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4837 gccaacggctccgtctccatgtccgccgcccaggtgggagcctctgccaacacactgcca 4896 *F Query: 121 caagtattcgttaaacccagcctgccgcagcccagcgctggcggcgatgtggtgaccacg 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4897 caagtattcgttaaacccagcctgccgcagcccagcgctggcggcgatgtggtgaccacg 4956 *F Query: 181 actggagctgctcgcccatccacgcaacaactgcccacaaagttcatt 228 *F |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4957 actggagctgctcgcccatccacgcaacaactgcccacaaagttcatt 5004 # *U FBrf0138594 *a Levis *b R.W. *t 2001.5.22 *T personal communication to FlyBase *u *F Date: Tue, 22 May 2001 12:55:58 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: CG12822 and CG18853 \- one or two genes? *F It appears from GeneSeen and Gadfly that CG12822 and CG18853 share *F their first two exons. Why are they assigned two CG's rather than *F being considered alternative products of a single gene? *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0138595 *a Nelson *b D. *t 2001.8.22 *T personal communication to FlyBase *u *F Date: Wed, 22 Aug 2001 12:25:39 \-0500 *F From: David Nelson *F Subject: Drosophila Cyp318a1 finished *F To: 'Michael Ashburner (Genetics)' *F Dear Michael, *F I was going over the Drosophila P450s and found that Cyp318a1 on *F gi|7292734|gb|AE003488.1|AE003488 still had a small gap between *F 16160 NLHGQAVKFTAAEDLLSRAVLEVS 16231 and *F 16820 EESKKCAKLLEDFVGGIVRTKHRNWRLRDAVGGEKSGEDASNGWQRRIFIEQIFQLAANG 16999 *F this is shown as a run of NNNNNNN in the sequence *F I searched for matches to the adjacent sequence and found the missing piece *F on AC023731. I have tried to reassemble the protein from the genomic DNA *F but this one is a hard one to do since there is no good model for it. I *F also removed a short in frame intron to remove 20 aa that seemed too long *F compared to the best matches of other P450s. The shorter sequence gave a *F more satisfactory blast match. The revised protein sequence is shown *F below. There are ESTs for this gene but they do not cover the middle. *F David Nelson *F >Cyp318a1 AC014186 comp(968-207) rev 8/22/2001 AC023731 has missing piece *F MCCLQSTTLDRKKNASWNRSAIVGSDTSDAPEHCPLRCGALLAVLLAWQQRKCWRLIWQL *F NGWRGVIQQPVLWLLLCINLHPNSILEKVSQYRVHFQRPLRVLVGTRVLLYIDDPAGMEC *F VLNAPECLDKTFLQDGFFVRRGLLHARGQKWKLRRKQLNPAFSHNIVASFFDVFNSVGNQ *F MVEQFQTQTNLHGQAVKFTAAEDLLSRAVLEVS *F missing part shown below from AC023731 *F LTIMGTPTNFTQLDDAHIAHSYKR *F LLEISAVRVVKPWLQIRLLHRLLAPELY *F EESKKCAKLLEDFVGGIVRTKHRNWRLRDAVGGEKSGEDASNGWQRRIFIEQIFQLAANG *F EMTLEEIMDEAQSMVLV (removed intron here) SFETVSNSIMLALLCLATNKGDC *F QRRLLAEIRALVPDVGQVGLEQLQQLRYLDAFVSESLRLLATVPMNLRHVSRDFRLAGRQ *F HETIVPQNSIVVLDTFNMQRDERWWGANARQFDPQRFLDQEEEQLSKGHNDSGSGEKRRQ *F RDRRHSYSFLPFSNGLRSCIGRRYGLFIMKVFLVKLITNFDFQSDFELEKLQFVENISLK *F FKNADDILLTIQPKKEST # *U FBrf0138596 *a Gelbart *b W.M. *t 2001.8.21 *T personal communication to FlyBase *u *F Date: Tue, 21 Aug 2001 11:43:06 \-0400 (EDT) *F From: William Gelbart *F To: flybase-updates@morgan.harvard.edu *F Subject: Merging of HMS-Beagle and midline-jumper *F Dear FB-Curators, *F I think that the natural transposable element called midline-jumper is *F actually an internally deleted version of HMS-Beagle. *F I compared AF365402.1, a 7062 bp sequence of an HMS-Beagle element, *F with AF315785.1, a 4617 bp sequence of a midline-jumper element. *F The results are that their 266 bp LTRs are 100% identical *F (both 5' and 3'), and that the following alignments are *F observed: *F 3Õ-5Õ 5Õ-3Õ *F LTR match LTR match *F HMS-Beagle (AF365402.1): 1-882 3332-7062 6797-7062 1- 266 *F midline-jumper (AF315785.1): 1-882 877-4617 1- 266 4352-4617 *F % identity : 100% 99% 100% 100% *F matches/ total bases : 882/882 3730/3741 266/266 266/266 *F gapped bases : 0/882 10/3741 0/266 0/266 *F Thus, 877-3331 (or 882-3336 as you wish) of HMS-Beagle (AF365402.1) is *F missing from midline-jumper (AF315785.1). So I think it is most likely *F that midline-jumper is an internally deleted version of HMS-Beagle and *F that the two entries should be merged. *F My guess is that because both sequences were submitted within 3 months *F of each other earlier this year, the sequence similarity was missed by *F each of the author groups. *F Respectfully yours, *F Bill Gelbart *F ========================================================================= *F Actual alignment data: *F Score = 1696 bits (882), Expect = 0.0 *F Identities = 882/882 (100%) *F Strand = Plus / Plus *F Query: 1 agttattgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgcca 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 agttattgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgcca 60 *F Query: 61 tcaacgccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 tcaacgccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacg 120 *F Query: 121 cctgctgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgt 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 cctgctgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgt 180 *F Query: 181 acgctagatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaat 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 acgctagatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaat 240 *F Query: 241 caaatcgataactgtaattattaactggcgcccgaacagggaccagcgaataaacgcgaa 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 caaatcgataactgtaattattaactggcgcccgaacagggaccagcgaataaacgcgaa 300 *F Query: 301 cgaaagacaaaattctaagtcgcggagcaaaatcaaaattttgctaaaaaatattcgttg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301 cgaaagacaaaattctaagtcgcggagcaaaatcaaaattttgctaaaaaatattcgttg 360 *F Query: 361 gttaaattgtgccgaagaaactcccgcgagttattaacaaacaaaattcacggtcggcta 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 361 gttaaattgtgccgaagaaactcccgcgagttattaacaaacaaaattcacggtcggcta 420 *F Query: 421 taaaataaaattgtttgagaaaaaaacagattttccggaagaggaaaatacttatcggca 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 421 taaaataaaattgtttgagaaaaaaacagattttccggaagaggaaaatacttatcggca 480 *F Query: 481 tagcattgcccattggtggatcacagtttctgtcaggccagcccgcagaaaaccttcttt 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 481 tagcattgcccattggtggatcacagtttctgtcaggccagcccgcagaaaaccttcttt 540 *F Query: 541 ggagtgctgacgtggattccccagtagcccaggcaaaaaggacaacattcacccagccag 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 541 ggagtgctgacgtggattccccagtagcccaggcaaaaaggacaacattcacccagccag 600 *F Query: 601 gaggagtgaccgcaacaattctatgttaaataaatgcaaaacgaagaacagcaatttcat 660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 601 gaggagtgaccgcaacaattctatgttaaataaatgcaaaacgaagaacagcaatttcat 660 *F Query: 661 cgaaaaggagaaggaaaagtagaagtagaacgccgagaataacggaactacagaagagca 720 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 661 cgaaaaggagaaggaaaagtagaagtagaacgccgagaataacggaactacagaagagca 720 *F Query: 721 acacggataacagttaacagtgcaacccaaaacgtaacggcagaggtaaagacgacgaac 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 721 acacggataacagttaacagtgcaacccaaaacgtaacggcagaggtaaagacgacgaac 780 *F Query: 781 ggcagagaacggctgcgtgaaccaaagtgcagacaagaaggaaaaaaaaagaacaacaac 840 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 781 ggcagagaacggctgcgtgaaccaaagtgcagacaagaaggaaaaaaaaagaacaacaac 840 *F Query: 841 agcacagccgtagcagcagcagcagcccgccaaaaagaacag 882 *F |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 841 agcacagccgtagcagcagcagcagcccgccaaaaagaacag 882 *F ============================================================================ *F Score = 7033 bits (3658), Expect = 0.0 *F Identities = 3730/3741 (99%), Gaps = 10/3741 (0%) *F Strand = Plus / Plus *F Query: 3332 *F gaacagagaatgatattgaataaaataattgattcgtatccaggtctcttcgcagacccg 3391 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 877 *F gaacagagaatgatattgaataaaataattgattcgtatccaggtctcttcgcagacccg 936 *F pol polyprotein 1 M I L N K I I D S Y P G L F A D P *F Query: 3392 *F aatcaaaaactaacctacacaacaagtgtaagggcagcaatccgaactatatcggatacg 3451 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 937 *F aatcaaaaactaacctacacaacaagtgtaagggcagcaatccgaactatatcggatacg 996 *F pol polyprotein 18 N Q K L T Y T T S V R A A I R T I S D T *F Query: 3452 *F ccaatatactcaaaattctatcagtacccaatgtctcttaaagacgaggtacacaaacaa 3511 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 997 *F ccaatatactcaaaattctatcagtacccaatgtctcttaaagacgaggtacacaaacaa 1056 *F pol polyprotein 38 P I Y S K F Y Q Y P M S L K D E V H K Q *F Query: 3512 *F atttccgaacttttacacgatggaatcattcgaccctcaaggtcaccttacaattcacca 3571 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1057 *F atttccgaacttttacacgatggaatcattcgaccctcaaggtcaccttacaattcacca 1116 *F pol polyprotein 58 I S E L L H D G I I R P S R S P Y N S P *F Query: 3572 *F gtgtggattgtaccaaaaaaactcgactcctctggcaagaaaaaatacagggtggtaatc 3631 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1117 *F gtgtggattgtaccaaaaaaactcgactcctctggcaagaaaaaatacagggtggtaatt 1176 *F pol polyprotein 78 V W I V P K K L D S S G K K K Y R V V I *F Query: 3632 *F gactatcgaaaacttaacatggtaacggtagcggacagataccctatccctgacattaat 3691 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1177 *F gactatcgaaaacttaacatggtaacggtagcggacagataccctatccctgacattaat 1236 *F pol polyprotein 98 D Y R K L N M V T V A D R Y P I P D I N *F Query: 3692 *F gaagtgttggcccaattgggagacaacaagattttctcagtgctcgatctgaaaagtggg 3751 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1237 *F gaagtgttggcccaattgggagacaacaagattttctcagtgctcgatctgaaaagtggg 1296 *F pol polyprotein 118 E V L A Q L G D N K I F S V L D L K S G *F Query: 3752 *F tttcatcagattcttttaaaggaatctgatatcgaaaagaccgccttctccatcaataat 3811 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1297 *F tttcatcagattcttttaaaggaatctgatatcgaaaagaccgccttctccatcaataat 1356 *F pol polyprotein 138 F H Q I L L K E S D I E K T A F S I N N *F Query: 3812 *F ggaaaatatgagtttacacgactcccattcggtctgaaaaatgcaccgtcaattttccag 3871 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1357 *F ggaaaatatgagtttacacgactcccattcggtctgaaaaatgcaccgtcaattttccag 1416 *F pol polyprotein 158 G K Y E F T R L P F G L K N A P S I F Q *F Query: 3872 *F cgcgcactggacg-atattcttcacgaacatatcgg-taagatatgtttcatttatattg 3929 *F ||||||||||||| |||||||||||||||||||||| *F ||||||||||||||||||||||| *F Sbjct: 1417 *F cgcgcactggacggatattcttcacgaacatatcgggtaagatatgtttcatttatattg 1476 *F pol polyprotein 178 R A L D G Y S S R T Y R V R Y V S F I L *F Query: 3930 *F acgacatcatcatcttt-agtaaagatgatgaaacacattaccagaaccttgacactatt 3988 *F ||||||||||||||||| *F |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1477 *F acgacatcatcatcttttagtaaagatgatgaaacacattaccagaaccttgacactatt 1536 *F pol polyprotein 198 T T S S S F S K D D E T H Y Q N L D T I *F Query: 3989 *F ttcagaactcttcagcaagccaacatgaaatgtcagttggataaatgcgagttcatgaag 4048 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1537 *F ttcagaactcttcagcaagccaacatgaaatgtcagttggataaatgcgagttcatgaag 1596 *F pol polyprotein 218 F R T L Q Q A N M K C Q L D K C E F M K *F Query: 4049 *F agaaaagtggagttcctaggcttcgtcgtgtccgacaagggcattgaaaccagcccaacc 4108 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1597 *F agaaaagtggagttcctaggcttcgtcgtgtccgacaagggcattgaaaccagcccaacc 1656 *F pol polyprotein 238 R K V E F L G F V V S D K G I E T S P T *F Query: 4109 *F aaggtacaggcaatctcagactttccaattccaaggacactcaaagaactgagatcattc 4168 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1657 *F aaggtacaggcaatctcagactttccaattccaaggacactcaaagaactgagatcattc 1716 *F pol polyprotein 258 K V Q A I S D F P I P R T L K E L R S F *F Query: 4169 *F ttgggattatccggatattacaggcgatttattcctaactacgctaagttagcaaaacca 4228 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1717 *F ttgggattatccggatattacaggcgatttattcctaactacgctaagttagcaaaacca 1776 *F pol polyprotein 278 L G L S G Y Y R R F I P N Y A K L A K P *F Query: 4229 *F cttagctcgcttttgagaggggaggatggtcgaatttccaggacattatcgtcaaaaaaa 4288 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1777 *F cttagctcgcttttgagaggggaggatggtcgaatttccaggacattatcgtcaaaaaaa 1836 *F pol polyprotein 298 L S S L L R G E D G R I S R T L S S K K *F Query: 4289 *F tccgtctcccttaatcgcgaagcaatagaagccttcaagaaattgaagagcagcttggtt 4348 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1837 *F tccgtctcccttaatcgcgaagcaatagaagccttcaagaaattgaagagcagcttggtt 1896 *F pol polyprotein 318 S V S L N R E A I E A F K K L K S S L V *F Query: 4349 *F tctccagacgtaatactccactacccggactttaagaaagaatttcacctaacaacggat 4408 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1897 *F tctccagacgtaatactccactacccggactttaagaaagaatttcacctaacaacggat 1956 *F pol polyprotein 338 S P D V I L H Y P D F K K E F H L T T D *F Query: 4409 *F gcttccaatttcgcagtaggtgctgttctttcacaagagaacagacccatctcattttta 4468 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1957 *F gcttccaatttcgcagtaggtgctgttctttcacaagagaacagacccatctcattttta 2016 *F pol polyprotein 358 A S N F A V G A V L S Q E N R P I S F L *F Query: 4469 *F tcgagaacactctcgaaggcggaagaaaattatgccacgaatgagaaggaaatgttagcc 4528 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2017 *F tcgagaacactctcgaaggcggaagaaaattatgccacgaatgagaaggaaatgttagcc 2076 *F pol polyprotein 378 S R T L S K A E E N Y A T N E K E M L A *F Query: 4529 *F attatctgggctctaaaaaagctaaaaatttacctttacggtaaagcaaaggtgaaaatc 4588 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2077 *F attatctgggctctaaaaaagctaaaaatttacctttacggtaaagcaaaggtgaaaatc 2136 *F pol polyprotein 398 I I W A L K K L K I Y L Y G K A K V K I *F Query: 4589 *F tttactgaccatcagcctttgacccattctctcagtagttggaatggaaatgc-gaggat 4647 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| *F |||||| *F Sbjct: 2137 *F tttactgaccatcagcctttgacccattctctcagtagttggaatggaaatgccgaggat 2196 *F pol polyprotein 418 F T D H Q P L T H S L S S W N G N A E D *F Query: 4648 *F taagagatggaaagcataccttgaggaatatgactacgaaattttctacaagccaggcag 4707 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2197 *F taagagatggaaagcataccttgaggaatatgactacgaaattttctacaagccaggcag 2256 *F pol polyprotein 438 ^^^ *F Query: 4708 *F agaaaatactgtagccgacgctctgtccagaggaccgacagtcgaacaaattaacacagt 4767 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2257 *F agaaaatactgtagccgacgctctgtccagaggaccgacagtcgaacaaattaacacagt 2316 *F Query: 4768 *F agcctcaacaatgcacagctctgacagttcgagccatgggctgatacc-tagcgttgaag 4826 *F |||||||||||||||||||||||||||||||||||||||||||||||| *F ||||||||||| *F Sbjct: 2317 *F agcctcaacaatgcacagctctgacagttcgagccatgggctgataccctagcgttgaag 2376 *F Query: 4827 *F ccccgataaacgcattcaagaatcaaattttcttcagggagtccgagtcagagaactact 4886 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2377 *F ccccgataaacgcattcaagaatcaaattttcttcagggagtccgagtcagagaactact 2436 *F Query: 4887 *F catttagcattccattcccgacatttcaaaggcatttagtagatcgcaacttgttcacac 4946 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2437 *F catttagcattccattcccgacatttcaaaggcatttagtagatcgcaacttgttcacac 2496 *F Query: 4947 *F ccgatagtctcttgtcagatttgaaagaatatcttaacccatccgtgattaatggaattt 5006 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2497 *F ccgatagtctcttgtcagatttgaaagaatatcttaacccatccgtgattaatggaattt 2556 *F Query: 5007 *F tcacatccgaggatgtaatggggaaaattcaaattctctaccccatccattttcagggtt 5066 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2557 *F tcacatccgaggatgtaatggggaaaattcaaattctctaccccatccattttcagggtt 2616 *F Query: 5067 *F tcaagattagattctgccaaagcaaagtcaaagacctt-gttaacgaagccgaacaagag 5125 *F |||||||||||||||||||||||||||||||||||||| *F ||||||||||||||||||||| *F Sbjct: 2617 *F tcaagattagattctgccaaagcaaagtcaaagacctttgttaacgaagccgaacaagag 2676 *F Query: 5126 *F gaagaaatacttaggacacataacagagcacacagaaatgctgtggaaaataaagctcag 5185 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2677 *F gaagaaatacttaggacacataacagagcacacagaaatgctgtggaaaataaagctcag 2736 *F Query: 5186 *F ttgtccgaaagagtatactttcctaaaatgaggaaaaaagtttcggctatcgtgaatcag 5245 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2737 *F ttgtccgaaagagtatactttcctaaaatgaggaaaaaagtttcggctatcgtgaatcag 2796 *F Query: 5246 *F tgtttggtgtgtaagactgctaaatatgacagacatcccacgcatccagaaataagacaa 5305 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2797 *F tgtttggtgtgtaagactgctaaatatgacagacatcccacgcatccagaaataagacaa 2856 *F Query: 5306 *F actcccttgccagaataccccggacaaattattcatattgacat-ctactcgacagaacg 5364 *F |||||||||||||||||||||||||||||||||||||||||||| *F ||||||||||||||| *F Sbjct: 2857 *F actcccttgccagaataccccggacaaattattcatattgacattctactcgacagaacg 2916 *F Query: 5365 *F acatctggtgctcacggcgattgataaattttccaaactggccatgggaagagttatcaa 5424 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2917 *F acatctggtgctcacggcgattgataaattttccaaactggccatgggaagagttatcaa 2976 *F Query: 5425 *F atccaaagctgtagaagacattaggaaagccctaagagatat-cgtgttttattatggag 5483 *F |||||||||||||||||||||||||||||||||||||||||| *F ||||||||||||||||| *F Sbjct: 2977 *F atccaaagctgtagaagacattaggaaagccctaagagatattcgtgttttattatggag 3036 *F Query: 5484 *F tgcctaaattaatagtaatggacaatgaaaagtccctcaactcagcctctatcaaattca 5543 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3037 *F tgcctaaattaatagtaatggacaatgaaaagtccctcaactcagcctctatcaaattca 3096 *F Query: 5544 *F tgttgacagaccagctgggtattgagctctacaaagcacctccgtataagagtacagtaa 5603 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3097 *F tgttgacagaccagctgggtattgagctctacaaagcacctccgtataagagtacagtaa 3156 *F Query: 5604 *F atggacagatagaaagatttcactccacactctctgaaataatgagatgtttgaaaggag 5663 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3157 *F atggacagatagaaagatttcactccacactctctgaaataatgagatgtttgaaaggag 3216 *F Query: 5664 *F atggaacacataggggcttcgaggaacttctcgatagggccatctatgaatataactaca 5723 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3217 *F atggaacacataggggcttcgaggaacttctcgatagggccatctatgaatataactaca 3276 *F Query: 5724 *F ctgtccatt-ctgtcacaaaaaaa-cgacctctagaggtgttcttcggcaggatagctac 5781 *F ||||||||| |||||||||||||| *F ||||||||||||||||||||||||||||||||||| *F Sbjct: 3277 *F ctgtccatttctgtcacaaaaaaaacgacctctagaggtgttcttcggcaggatagctac 3336 *F Query: 5782 *F cgtagctccagaaaaatatgaacaagctagactggacaatatagaccgacttaggcaaaa 5841 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3337 *F cgtagctccagaaaaatatgaacaagctagactggacaatatagaccgacttaggcaaaa 3396 *F Query: 5842 *F acaggaaaccgacatagaataccacaaccggacaagaaagcccataaaaacctatatcaa 5901 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3397 *F acaggaaaccgacatagaataccacaaccggacaagaaagcccataaaaacctatatcaa 3456 *F Query: 5902 *F agggcaagaaattttcgttagggttaatacaagattaggttctaagttatcaagtagatt 5961 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3457 *F agggcaagaaattttcgttagggttaatacaagattaggttctaagttatcaagtagatt 3516 *F Query: 5962 *F taggaaggaactagttaaggaagaccgaagtaccacaatattaacagaatcagggaaatt 6021 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3517 *F taggaaggaactagttaaggaagaccgaagtaccacaatattaacagaatcagggaaatt 3576 *F Query: 6022 *F agtacataaaagtaacataagatcatgattgcttcagaataattactgtggccacatcga 6081 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3577 *F agtacataaaagtaacataagatcatgattgcttcagaataattactgtggccacatcga 3636 *F Query: 6082 *F tagacaagtagaacatagaaacattaaacatactgattaacgtaaccacgagaattaaat 6141 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3637 *F tagacaagtagaacatagaaacattaaacatactgattaacgtaaccacgagaattaaat 3696 *F Query: 6142 *F ttgaaacgattcgactttgacagacgagttggccataggtttgcagaattagattgggct 6201 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3697 *F ttgaaacgattcgactttgacagacgagttggccataggtttgcagaattagattgggct 3756 *F Query: 6202 *F cgtgaaagaagagattgcgttgtgaatgcgtttctgcggagcgagtttaaattgaatgag 6261 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3757 *F cgtgaaagaagagattgcgttgtgaatgcgtttctgcggagcgagtttaaattgaatgag 3816 *F Query: 6262 *F accagtagcctacttgaaaacaatataccgatttctgccttgacggtcaaataaatgtta 6321 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3817 *F accagtagcctacttgaaaacaatataccgatttctgccttgacggtcaaataaatgtta 3876 *F Query: 6322 *F aaactttcacacgttctcgctttacacaacggaactacaatttgaatgccattgaaatct 6381 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3877 *F aaactttcacacgttctcgctttacacaacggaactacaatttgaatgccattgaaatct 3936 *F Query: 6382 *F ccatattgaatcagttaattccttatttcttatattttaaaacatgatcagtaaaggaaa 6441 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3937 *F ccatattgaatcagttaattccttatttcttatattttaaaacatgatcagtaaaggaaa 3996 *F Query: 6442 *F ctaataataataaataaataataatttaatttgaataataataaaatttaccaattaaag 6501 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3997 *F ctaataataataaataaataataatttaatttgaataataataaaatttaccaattaaag 4056 *F Query: 6502 *F acgaaatgtacggaatcaatgccttctgcaaaggttccaatggtctaaaaatatgtagat 6561 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4057 *F acgaaatgtacggaatcaatgccttctgcaaaggttccaatggtctaaaaatatgtagat 4116 *F Query: 6562 *F ataaattcattagtaatttagtaaaaaccagaattttaatagaagcaatcaagttgtcaa 6621 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4117 *F ataaattcattagtaatttagtaaaaaccagaattttaatagaagcaatcaagttgtcaa 4176 *F Query: 6622 *F tgcagaacacatccgagttgcacaaacctcgaacgcacgcaacaataaacacctggcatt 6681 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4177 *F tgcagaacacatccgagttgcacaaacctcgaacgcacgcaacaataaacacctggcatt 4236 *F Query: 6682 *F caaaccattaatgctgtgcccaaactaccacgaatcaccccaccaacgatgcgactcaaa 6741 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4237 *F caaaccattaatgctgtgcccaaactaccacgaatcaccccaccaacgatgcgactcaaa 4296 *F Query: 6742 *F aacatcccatccttatgatgaccgctcgaggacaagcgtcaattaagaggggaggagtta 6801 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4297 *F aacatcccatccttatgatgaccgctcgaggacaagcgtcaattaagaggggaggagtta 4356 *F Query: 6802 *F ttgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgccatcaac 6861 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4357 *F ttgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgccatcaac 4416 *F Query: 6862 *F gccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacgcctgc 6921 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4417 *F gccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacgcctgc 4476 *F Query: 6922 *F tgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgtacgct 6981 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4477 *F tgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgtacgct 4536 *F Query: 6982 *F agatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaatcaaat 7041 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4537 *F agatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaatcaaat 4596 *F Query: 7042 cgataactgtaattattaact 7062 *F ||||||||||||||||||||| *F Sbjct: 4597 cgataactgtaattattaact 4617 *F =============================================================================== *F ======= *F Score = 512 bits (266), Expect = e-141 *F Identities = 266/266 (100%) *F Strand = Plus / Plus *F Query: 6797 agttattgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgcca 6856 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 agttattgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgcca 60 *F Query: 6857 tcaacgccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacg 6916 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 tcaacgccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacg 120 *F Query: 6917 cctgctgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgt 6976 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 cctgctgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgt 180 *F Query: 6977 acgctagatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaat 7036 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 acgctagatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaat 240 *F Query: 7037 caaatcgataactgtaattattaact 7062 *F |||||||||||||||||||||||||| *F Sbjct: 241 caaatcgataactgtaattattaact 266 *F =============================================================================== *F ======= *F Score = 512 bits (266), Expect = e-141 *F Identities = 266/266 (100%) *F Strand = Plus / Plus *F Query: 1 agttattgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgcca 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4352 agttattgccctgcaattgattctctaacatcttgtggttccacatagtctccgctgcca 4411 *F Query: 61 tcaacgccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4412 tcaacgccaacgaacggttaagcgcgacatcgacacttctgcgctgcgccgcggccgacg 4471 *F Query: 121 cctgctgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgt 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4472 cctgctgcgccgctgccgacgacttcacttgattgctagggacttagggaaacattttgt 4531 *F Query: 181 acgctagatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaat 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4532 acgctagatttagtttcaaatgataaattgcaataaacggtcgcttgcgatcttcaaaat 4591 *F Query: 241 caaatcgataactgtaattattaact 266 *F |||||||||||||||||||||||||| *F Sbjct: 4592 caaatcgataactgtaattattaact 4617 # *U FBrf0138599 *a O'Hare *b K. *t 1992.6.24 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Tue Sep 18 19:29:36 2001 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 18 Sep 2001 19:29:36 \+0100 *F Date: Tue, 18 Sep 2001 13:20:36 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: P{} Insertions in sn *F To: gm119@gen.cam.ac.uk *F Cc: matthewk@fly.bio.indiana.edu, flybase-archive@morgan.harvard.edu *F Personal communication from: Kevin O'Hare, Imperial College of Science, *F Technology and Medicine, London *F To: Bloomington Drosophila Stock Center *F Subject: P{} Insertions in sn *F Dated: 24 June 1992 *F Background: The following information relevant to FlyBase curation was *F provided with stocks contributed to the collection. *F Information communicated: *F snPlacz,ry+1 \- homozygous viable and fertile, extreme bristle phenotype; *F made by Jamie Paterson and Kevin O'Hare by jumping a copy of the original *F O'Kane and Gehring Placz,ry+A transposon into sn *F snPlacz,ry+2 \- homozygous viable, females poorly fertile when homozygous, *F extreme bristle phenotype; independent insertion made as above *F snAL2 \- homozygous viable and fertile, made by Allan Lohe and Tony Mahowald, *F using the Bier element *F No difference in lacZ staining pattern has been detected among the three *F insertions. snPlacz,ry+1 and snPlacz,ry+2 were mapped to the 5' exon *F of sn and transcription of lacZ is in the opposite direction of that of sn. A *F few blots were done with snAL2 and it does have a single insertion but that *F insertion was not mapped in more detail. *F The lacZ staining in adult ovaries is best defined. It begins in nuclei of *F border cells at stage 8, in nurse cell nuclei at stage 9 and in the oocyte *F cytoplasm at stage 11. There is some weaker staining of some follicle cells at *F the posterior end of the egg chamber. The embryos begin as uniformly stained *F and this becomes more diffuse as development proceeds. Larval stages show no *F specific staining. There is considerable staining during pupal stages and *F there is some staining in adult testes. # *U FBrf0138600 *a Robertson *b H.M. *t 2001.7.7 *T personal communication to FlyBase *u *F Date: Sat, 07 Jul 2001 17:52:34 \-0500 *F From: hughrobe@life.uiuc.edu *F Subject: Third set of annotation suggestions from bee ESTs *F To: Millburn_Gillian *F Dear Gillian, *F Attached is a TEXT file with the last set of suggestions for annotation *F improvements deriving from our honey bee EST project. .. *F Thanks, *F Hugh *F Hugh M. Robertson *F Professor *F Department of Entomology *F University of Illinois at Urbana-Champaign *F 320 Morrill Hall, MC118 *F 505 S. Goodwin *F Urbana, IL 61801 *F Phone 217-333-0489 *F FAX 217-244-3499 *F Email hughrobe@life.uiuc.edu *F www.life.uiuc.edu/robertson/lab.html *F \------------------------------------------------------------------------------ *F -- *F \**********A. mellifera BB260007A20E1.F *F TGGCTTTTAAAATAAATAATTTTTAGATTTTATCAAAAAGGTTAGTAAGTCCGGTCAGGTACGACGAGAATTTAAGTTT *F TCCAGTGCAGAATGTTTTGGAAAATGGTTGGTGAACCGGAGAACAACAAGCAGAGCCATTTAAAGCTATAAGATTTAAT *F CTGAACGGAAATGGAAAATTATTTTCCCAAGGTGAAATGGAAAATTAATTTCCCAATCATTCAGTTTAATAGATTTTTA *F TATTTTAAATATCATGCTATTATTTTATTTTACACAGATTAAGGGAATTGGATAGAGATGAACTTTTCGAAAAAGCAAG *F AGGAGAAATCTTGGATGAAATTGTAAATCTGTCCCAAGTTTCGCCTAGACACTGGGAAGAAGTGTTAATGGTCAGAATT *F TGGGATAAAGTTAGTATGCACGTATTTGAAAACATCTATCTACCCGCGGCTCAAAGCGGAAGTCCAAGTATATTTCACA *F ATAATTTTATATGATTTTCCCTTATGAATATTTACTATTATAATTATCATTATAATTATTTTTCTTTGTTATAGGTACA *F TTTAATACTACCGTTGATATAAAACTTCGTCAATGGGCTGAACAACAATTGCCAGCTCGAAGTGTCGAAAGTGGTTGGG *F AATGTTTACAACAGGAATTTCAACATTTTATGAATCAAGCAAAACTCAGTCCTGATCATGATGATATTTTTGATAATTT *F AAAAAATGCAGTAGTCAGTGAAGCTATGAGACGTCATTTTT *F Unspliced transcript, yet clearly shows that CG8479 is missing an exon, the *F sequence of which is also present in mammalian and nematode orthologs *F \**********A. mellifera BB260009B10G1.F RC *F GGTCATAGGGGTCAGGATCGAGGGTTATGCCGAATTTATCTTCGTTATGCTCAAAATTTTTTTCAATGAAACAATATAA *F TTAATGAATTTAAAAGTTTTAGAAATCCTGATTTTCAAGAAGCAGTTTTTCAAAAAGATGGTCAGATACTCTTAACATT *F TGCAATTGCTAATGGATTTAGAAATATACAAAATCTTGTACAAAAATTGAAGCGTGGAAAATGTCTATATGACTATGTT *F GAAATTATGGCATGTCCTTGTGGATGTCTTAATGGAGGAGCACAAATTAGACCTTTAAATAATGTTCAATCACGTGAAC *F TGGCATTAAAATTAGAATCTATATATCGAGAACTTCCTCAAAGTGATCCTGAACAAAATTTAATAGTGAAAAATTTATA *F CAAAAATTGGTTAGGAGGAGAATATACAGACAAAGCTTTAGCATATTTTCATACTCAATATCATGAAATAAAGAAAGTA *F AATACTGCTCTTGCTATAAAATGGTAATTTATGATAAATATATATTATTATTAATTTAAATTAGAATTTTTAAATACAA *F CTAATATCAAATTAATATGATTTAAAAATATAGTTGTATGATTAGATGTATATTTATTCTATTACTATTATATATTAAA *F AAACCTTAATTGTTGGCAAATATTCATTAAAGAATATAAAAGACAAATTTTTAATAAATATTTATATCTTTTATAAAAA *F AAAAAATAATTTAATAATATACATGTAAATATAAATTTGATATTATATAGAGTATAATGTATTTAATGTAAATTATCTA *F ATTATAAGGATATAAAAGAAATTTC *F The vertebrate matches for this are about 476aa, while the Drosophila CG17683 *F is only 2141, but when search with vertebrate proteins, Drosophila genome show *F N-terminus in exon just upstream of CG17683 *F \**********A. mellifera BB260011A10D4.F *F AATATTCCAGAAGGAACTCATCAATATGATTTGATGAAGCATGCAGCAGCCACTTTAGGTTCTGGTAATTTAAGACTTG *F CAGTTATGCTTCCAGAAGGTGAAGATTTGAATGAATGGGTTGCAGTAAATACTGTTGATTTTTTCAACCAAATCAATAT *F GTTATATGGCACTATCACAGAATTCTGTACTGAAGAAAGTTGTCCCATCATGTCTGCAGGACCAAAATATGAATACCAT *F TGGGCTGATGGGCATACTGTCAAAAAACCAATAAAATGTTCTGCACCAAAATATATTGATTATTTAATGACTTGGGTAC *F AGGATCAATTAGATGATGAAACCTTATTTCCTTCAAAAATTGGTGTTCCTTTTCCAAAAAACTTCTTGTCTATTGCTAA *F AACAATATTGAAAAGACTATTCAGAGTATATGCTCATATTTATCATCAACATTTTAGTGAAGTCGTTCAACTTGGCGAA *F GAAGCACATTTAAATACATCATTCAAACATTTTATATTTTTTGTTCAAGAATTTAATTTGATAGAAAGAAGAGAATTGG *F CACCATTGCAAGAATTAATAGAGAAGTTAACAGCAAAGGATGCTCGATGAATGATCTTGATAGTTAACTGAAAACTCCT *F CCAAAGAATCTCACTGTGCTATCTTTTTAACGTGTAATTGTGCATGTTATTTATTAATCCAATTCTTGCT *F This and excellent mammalian matches show that the N-terminus is missing from *F CG13852 *F \**********A. mellifera BB260015B20F5.F *F GTTGCCTTTTTTTTTTTTTTTTTTTTTTGCTCTGTACTTATTTGAATCTGTAGCTAACAGAGAGCTAATTTCAGAGATA *F TACTTCGCTATATAGAGGAGGATATTCTACCAGAAATGCATGTAAAATTTGGCCAAGAAATTCTATATTTAGAAGGATG *F GTGTGCACGAACTCAGTATAATGCGTGCTGTAGATTACTTGGTCCGGGAATAAATATTCATCTTGCGGAAAATCAACTT *F CTGCGCGAAATTTTTCACCTTGGTAACAAAGTTATGCCGATACAATTGAATCAAAAGACAAGTAAATTAGAAAGGACAT *F TAATGAATGCAGCTGCATTCAAAGCGCGCACTATTCAGCGTAATAAAAACCGAGACAAACGATCAGCTGCCTTGGCACC *F GTGATTCTTCAACTTTCTTTTTTTATATATATTAGATAATTTCTTTTAAAATAAGAAAAAAAAAAGCAACGC *F This EST and the vertebrate matches show that CG3098 and CG15401 need to be *F fused. *F \**********A. mellifera BB260017A20C9.F *F CCAGGATCTCAATTGCCGAGATGTCGTACCGCGGAATGCTGAGCACCTCGGACGGTTCGCCCGTCGAGCTGCGCGTCCA *F GGATGTGGACTCCTTTGACGGATCGATGATGTCCCATGCGGCCACCAATGCGGCCGGTCCGCTGGGCAGCAAGGCCAGC *F GGCAAGTCCCTCAACGCCTGCACCTCCATGTCCATGCCCCAAAAGCCGAGTTCCCTAAGGGCGACACCCAGCCGCTGCC *F CATGTGATCTCGCCGATTCACCGGTGGATGAGAAGGCCGAAGCTGATGTGGAGGTGACCAACGAGATCTCGGCGCCCTA *F TGAAGTGCCCCAATTTCCCATTGAACAGATCGAGAAGAAGCTGCAAATCCAGCGCCATCTCAATGAAAAGCAGGCTACA *F GGACCTAGACCAGTGGCCACAGCCGCCGTATTGGCCACAAACAGGGAATCATCCAGCAGCACCGAGGGCCGCGAGTCCG *F CCGTTACTATGGAGAGAAACGATGCGGACATTAACTTTCAACGGGTCTCAATTTCGGGTGAGGACACCAGTGGCGTACC *F GCTCGAGGATTTGGAGCGGGCCTCGACCCTGCTGATCGAGGCACTGCGCCTGCGTAGCCACTACATGGCCATGTCGGAT *F CAATCGTTTCCCTCGACCACGGCTCGATTCCTCAAGACAGTGAAGCTCAAGGATCG *F This EST and vertebrate matches and then a whole set of old and new Drosophila *F ESTs show that CG15762, CG11065, and CG11058 need to be fused. *F \**********A. mellifera BB260018B20A5.F *F GGGAGTAAATTCTGGTGCTCATCAGCTTGACCAGTTCAGCACAACAGAATTGACGCCCGAACATCAGAAGATTCTAATA *F GACATCAGACGAAAGAAGACGGAACTGCTACTCGAAATACAACAACTGAAAGATGAGCTGGGTGAAGTGGTGGCTGAAA *F TGGAGGCGATGGAGGGCGGTGGTCTCGCCGTCGATGAAACCAAGCCATCGAATAAGGCTAAGCAGACGTCGATCGGCCG *F TAAGAAGTTCAACATGGACCCTAAGAAGGGCATCGAATACCTGATCGAGCACAATCTGCTAGCGCCGACGCCCGAGGAC *F GTCGCCCAATTCCTCTACAAGGGCGAAGGTCTTAACAAAACGGCGATTGGCGATTACCTTGGTGAAAGACACGACTTCA *F ACGAGAGGGTGTTGAGGGCATTCGTCGAATTGCATGATTTCACCGATTTGATCCTCGTACAAGCTCTTCGACAATTTCT *F TTGGTCGTTCCGAATGCCCGGCGAAGCGCAAAAGATCGATGGATGGAGGAATGCTTCGCACAAAGGGACTGGCCGGTGA *F AATCCCATTTTTTACCAATTCCGACCCTTGGTACCTGGTCAGGTTGCGTTTATATGGTGGGAACCTCTTTTCCCAATCC *F GAGGGCAAGGATAACCTACGGGGAG *F This and vertebrate matches show that CG11633 and CG11628 need to be fused \- *F indeed Drosophila EST from clone AT31091 unifies them already *F \**********A. mellifera BB260020A10B2.F *F GCTTTTTTTACTGGTGGAGAAATAGTCAGTACTTTTGATAATCCTGATATGGTGAAACTTGGCAAATGTGATCTTATTG *F AACAGGTGATGATAGGTGAAGATACTTTATTACGATTTTCAGGAGTTCCTTTAGGAGAAGCTTGTACAGTAATCATTAG *F AGGTGCAACCCAGCAAATTCTTGATGAAGCTGAGAGGTCTTTACACGATGCACTTTGTGTATTATCAGCTACCGTTCGC *F GAATCAAGAATTGTTTATGGAGGAGGATGTAGTGAGATGATAATGGCTTGTGCTGTTATGAGAGCTGCTGCTTCTACTC *F CTGGAAAAGAATCAGTTGCAATGGAATCTTTTGCTCGAGCATTGCAACAATTACCTACTGTTATTGCTGATAATGCTGG *F TTATGATTCAGCTCAATTAATTAGCGAATTACGAGCTGCACATAATTCTGGTGCAAATACCATGGGTTTGGATATGGAA *F CGAGGAAAAGTAAGCTGTATGAAACAATTAGGAGTAACTGAATCTTGGGCTGTGAAGAGACAAGTTTTACTTAGTGCAG *F CAGAAGCAGCAGAAATGATTTTACGTGTGGATGATATTTTACGAGCAACGCCCAGAAAGCGTGTTAAAGATCGCGGACG *F TTGTAATTATAGGTTACATATAACAATGCTCATTTATTGCTT *F This and vertebrate matches show that CG7033 needs a C-terminus, and it is *F readily endocded after an intron; *F \**********A. mellifera BB260020A10C8.F *F AATTTCAATATTTAAAGTTACCTCTTCTTAGGAAATGAAAAGAAATATTGAAACTATATTGAACTTATTAAAATAGAAA *F AAATTTATCATAAAAACTCTTTTAATTGATACTTGCGATTTGTGATAGATGATTTGTATAGTACGATAAAAGGATGAGG *F ATAGCAAAACAATTTAAATATTTTATACTTGCGAATAATTATCTGTGTAAATATATTTAGGCTTAGATTTACGTCATGA *F TCCTATGATTGTACTCCAATATGCGTTAAATCATCAAAAATATCATCAAAATGCCACGTATAGATCCTCTATCTTTATT *F GAAATGTCTTAGTGTTTTATTGGGGCCAACTGGAGGTATAAAAAGTAAAGAAGAAGTTCATCGGTTGGCTAGTTTGATG *F ACAAAATTTTCCAAAAAACTTGTTTCAAAATGCATTTATATACAAATATTGAAAACTACAAATACAGATTTACTTAGTC *F AATTTATGGGTGCTGGAGGATGGAATCTCATACATATGTGGCTTACAGATGGTATTCTTGCAAAAAATTGGGCTTTAAT *F TCAAGAACTTCTAGAACTTTTATTATTATGCCCAGTAGATATAGAAAGATTAAAAAGCAACAATTGTCCAAAATTAATA *F AAAGGTCTATCAAAAGAAAGTAGTCATCAAAGTGGTAAAATGTTA *F Shows an N-terminus is needed for CG4124 \- it is annotated as mRNA, but not *F translated. There are ESTs from Drosophila and Bombyx mori also showing it. *F \**********A. mellifera BB260020A20G9.F *F TGGTTCAGAGACTTTGCACTAGTGTAGCTGCGCTTAGCCTCGCCCTAAGTGCTTGCGTTTTTTTTCCGCGTGCTGTCAT *F GGCGATCGACTTAAGTCGATTTTATGGTCATTTCAACACGAAACGTTCAGGAGATGCTTGTCGTCCATATGAGCCATTC *F AAATGTCCAGGAGACGATACATGCATTTCGATTCAATATCTGTGTGACGGAGCTCCCGATTGTCAAGATGGATATGATG *F AAGATTCGCGATTATGCACAGCTGCAAAACGACCACCAGTAGAGGAAACTGCTAGTTTTCTGCAGTCATTGTTAGCAAG *F TCATGGTCCAAACTATCTTGAAAAATTGTTCGGGACTAAAGCGCGGGATACTTTAAAGCCTCTTGGTGGAGTGAATACA *F GTTGCTATAGCGCTTTCCGAATCTCAGACGATCGAGGATTTTGGTGCAGCCTTGCATTTGTTACGCACAGATTTGGAAC *F ATTTGCGCTCCGTATTTATGGCAGTGGAAAATGGCGATCTAGGCATGTTGAAATCAATAGGTATTAAAGATTCTGAATT *F GGGAGATGTGAAATTTTTCCTTGAAAAGCTCGTGAAAACTGGTTTTCTCGACTGAACAAGCTTTTTTTTAAACGTAGGG *F GCTATAACGTATATACGTTGACTGTATCGTGTTATAATCGCACTAACATATTTCTAGAAAGATTGTTGGAAGATTCTTT *F CACTAATTTTTGCAAATGATCACCGCCTCTCTTTTCTTCATTATTACTCT *F Shows that CG7237 needs both N and C-terminal regions readily available in *F genome. *F \**********A. mellifera BB260020B20A4.F *F GTTGGCCAGGAGTCGGAGCACTCACGCATTGAAATCGAGGGAGCCGAGCCCGGAGCGAGACAGGGTGGGCGCAGAGAAG *F GATGGGGCTGCGTTAAGTTCGTGGGCACGGTACTTGAAGAACAAGTACGGGAATCGAACGACCAAAGATAAGGAGCCTT *F CGTCCTCTGCCTCGACGATTCCATCATCGAGTGGAAGCACCTCGAGGAGGTTATCGCTCGGATTACCTTTGAGGCACGG *F TGGCCAAACGTCCTTCGAATCTTCTGACGACGATCAAAAAAACCCGTCAGGCTCCCCCACGTCCCCTACGGCAGCTCCC *F GTTATACCCGCGGCAGCAGGTTCCTCCACTAGCAATGGGCGGAGGAGTCACTACTTGCTGAAGCGGCGGCAGCTGTTCA *F AGTTCGGGATGCGGGGGAGCGAACCCGGATGCTTTACTTGGCCCAGAGGCCTCGCGGTTGGCCCTGACAACTCCATCGT *F AGTGGCCGACAGCTCTAACCATCGTGTTCAAGTGTTCGACTGTAATGGGAACTTCATGAAGGAGTTCGGATCGTACGGC *F AGCGGCGAGGGTGAATTCGATTGCCTGGCCGGGGTAGCGGTGAACAGGATCGGGCAATACATCATAGCGGATCGTTACA *F ACCACAGAATTCAAGTTCTCGATCCTTCCGGTCGTTTCTGAGAGCGTTTGGCTCCCAAGTACCGCAGACGGGCGGTTTA *F ATTATTCTTGGGGAATACCACCGATGCTCTTGGATTATTT *F Shows that there are other alternative splices of CG15105, and there is a *F Drosophila EST that confirms this. *F \**********A. mellifera BB260021B10H4.F *F GCGTCATAAGATTTGGAAACCATTACGAATGTTAATTGTCAAAAAATCATTATATCAATGTTAATTATTTAATTAAATT *F GATTATATGAATTGCTAATAAAAGCAATTAATTTAATTATTAAATAATGGAAAAACAACCGTTAAATCCAAAAGATGAT *F AAACCTCCGTCTTACTCAGCGGCAACTGCACCAAAAGTAAATTGTTCATGGCAACCACCACCAGGTTACAATTCCACAC *F AATGTCAAGAATCTACTTCATGGCCTCCACCTCCAGGATATTATCCTAGTGCCAGTGAAACTAATAGTCATAACTATGT *F ACCTTCTTATGGCTCTACACAATCAACTACAATAATTATGCCAGAAATTATTTTAGTTGGAGGATGTCCTGCATGTAGA *F GTGGGCATTATGGAAGATGATTTTACATGTCTTGGACTACTATGTGCTATTCTATTCTTCCCAGTGGGCATAATTTGTT *F GTTTGTTATTAAAGACACGACGTTGTTCTAATTGTGGTGCATATTTTGGTTAATGTGTTACTATATTACAGTAACCATT *F TCTGAAACTAAACATATTATTAATCTAATTTTAATATAATTATGTAGCTAATATAATTATATGATATTGAAAAATAAGA *F ACATAGGCAATTAATTTTTTATTAATATTATATAATTATAATTATCTATGCATTTGCATTATTTGAGGAAAAATAATGC *F CTCAATCATTCATATAATATACAATAATTTGTTAAATAGAAGTTCATGAAATATTGACATATATACATTAAGAAGATCT *F CTATGCATTTAAGACTTTATAATTTAAG *F This and vertebrate matches show that N-terminus is missing from CG12012; *F Bombyx mori ESTs agree. *F \**********A. mellifera BB270008B10B6.F *F ATATTCTCTATTATTAATGTAAATAGTTATTAATTTTAAGTTTTATTTTGTTACAACTGGTACCTGATTAATTTGTAGA *F TAGTAACATAACGTTTAACATCATGCCTATTCTAAGGAAAAAATCTAGCAAAAAAGTAAATGTGGAAAATGGCAATGAC *F AATTTGATTGGAGATATAACTATCAATGATTTTGCAAATTCATCACGGATACACACAAGATTTAAAAAGGCAATAATAA *F ATGTCTCCTCTGAAGTGAGACAAAAAATAACAATAGATGAAGAAAGTTTTTTAAATGAAATGAATGAAAATTACCAAAA *F TAATTTGTATGATTCGAATAAGTCAAAGAAAAGTACTAAAGGAGTTTTAACAAATCAAAGAAATAAAAAGACAAATTAT *F TTTAAAAAAGATATAGAAGAACAAGAAGGTATAGATTATCCATTAGATATATGGTTTATTATATCTGAATATATCAGTC *F CTGAGGCTATAGGAAAATTTGCTCAAATATGTAGAAGTTCTTATTATGTAGTTTCAACTGGAAAATTTTGGTTTCATTT *F GTATAAATCTTATTATAAATTTGTTCCTGGTTTACCAGAACGTCTACAACCACAATGTATGGTTCGTACTCATGGACTT *F CGAGCTTGTGTCATCAGAACATTACATTATACTTATTTTGCTTTGAAAAGAAAAGTTGATGATGTATCCTATTTAAGAA *F CAGATGAACCACATTCACTTATAAAA *F This and a new testes cDNA suggest that CG12765 needs a longer N-terminus. So *F do the vertebrate matches in mouse. *F \**********A. mellifera BB270010A10B7.F *F CTGTTCGGCGGCGGTGTTATTCGTACGGCCAATGAGGAAAGCGGAGCACGTGACCATGCTGGATCCTTTTCAAGAGAGA *F TACGGTGCCGGAGTGGGGGGTCTCTTGTTCCTGCCTGCCCTCTTCAGTGATCTGTTCTGGTGTGGCGGCGTGTTGAGAG *F CTTTGGGAAGCTCGTTGGCAGTGGTTGCTGGCGTGAATCCCGACATCAGCATAGTCGCCTCTGCCCTCTTGGCAGCCGT *F ATACACAGTGTTCGGTGGGCTTTACTCCGTCGCGTGCACCGATGCGTTGCAGCTGGCATGCATCGTGATCGGGTTGGGA *F TTGGCCGCGCCATTTTCCGTTCTCCACCCCGCCGTCAACTTCGAGAAAAATCTAACGCCGCACGAATGGCTCGGGGAGA *F TCAAGAACGAGGATCTCGGCGAGTGGGTGGATTGCATGCTGTTGTTGGTATTTGGCGGTATACCCTGGCAGGTATATCG *F AATATTATATTCGTCAAGATAATTCTGATTTGTGAATACGAATCGCGTGAATCTACACGAGCGTGAATAGATACTCGAT *F ACGCGCGAACTCTCGTTTC *F This and vertebrate matche indicate that CG7708 needs a different C-terminus, *F and it's there in the genomic matches. *F \**********A. mellifera BB270020A10D10.F *F TGGCCGTACACAACCAGAACTTATGGGAAGAGAACGACATGCAAAAACATTGGAAATTGCTCAAGAAGAGGTACTTACA *F TGTTTAGGAATGTGTGTTGCTGAACGTTTACATAGAGTTCATAGACGATTAAGAGAAGAGGAAACTGTATGCAAAGTGT *F TAGCTGCTGTTGCAGTAGATGCATTATCTAGAAATTTTCAAATGGCTGTAGAAGTTAAACAAGGTATTTCTCAATTAGA *F ACTTCTCTATGAAGAATTAACAAGAGAAGAGATAGCAAAACAACAAAGACGAGAAAAGTTACGTTTGAAACGTAAAAAG *F AAGAAAGAACGACGATATGAGACAGAAGAAAAAGAAAATACATGTGATGTATGTTATTATTTGAAAATTAAAATTTTCA *F AAATTTATATATTTAATATTCTTACATAAGTTAATTATTATTAGTGTTCGAGCAAAAAACAAAGTGGTAATAGTGATAC *F ATCTTGTGTTTGTGCGGATTCAAAACCGACAACACAAAATATAGATCAACATAAGTTACAAGTATTAGATCCAAAAAAT *F AAGGGACCACCTACTTGTAATGTCCGGATTGTGTAAAAAAATCAAAATCTAGTATATCACGTTCACAGAGTCAAACACA *F ATTAGCATTCCCAAAAAAATCATCGAACGTGCAAAAAACTACAATTAAAAAGAGTTCTTCTGAATCAAAGACCAATTTC *F TACAAT *F This and a B. mori EST, and now two Drosophila EST sfrom testes and Schneider *F cells, and vertebrate match indicate there is a segment missing from CG2182, *F and it's there in the genome. *F \**********A. mellifera BB270023A20B8.F *F CATATCGTATGTTGGTTATATACGAATAGTTTAAGATATCTACCAGTATTGGTAAGGCAATGGTGGAGTACTGCTGATA *F GTAGAGTCAGCGCTGCCGTGGATAAGATCACAACACATTATGTTAGCCCTATGCTTTGTCAAGAGGAACTTCTCAATAA *F TAAATTACAAAATATTGAAAACATGCAAGTAAAAGTACATCCAACATTCCGTGAAGTGATAGCTTTATATCAAATGGAT *F GATACAAAATTAGAACTTAATATTACATTGCCATCTAATCATCCTTTGGGACCAGTTAGCGTTGAACCTGGACAACACG *F CAGGTGGTACTGCGAATTGGCGGAATTGTCACATGCAATTATCCATATTTTTTACACATCAAAATGGATCTGTTTGGGA *F TGGACTTGCATTATGGAAAAGAAATTTAGATAAGAAATTCGCCGGCGTTGAAGAATGTTACATATGTTTCAGTATTTTT *F CACATAAATACGTATCAAATACCAAAATTATCTTGTCACACATGTCGTAAGAAATTTCATACTGCATGCTTGTATAAAT *F GGTTTAGTACAAGTCAAAAATCCACGTGTCCAATTTGTAGAAATATATTTTAATCTATTATATTATTTATAAACAAAAT *F TTGTATTTGTATTTAAATAAATAAAATAATATCTGTACATAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGTGCCGCC *F TCGTGCC *F This encodes a protein with an excellent match to the end of a very long human *F protein \- suggesting that the annotation of CG9274 needs revision \- and *F there is now a Drosophila Schneider cell EST matching it too. *F \**********A. mellifera BB270023B20C3.F RC *F GTTGGGCAACGTGAACAAGATAATAATACGGCCGCCAGGGCATAGCGGGAAGGCGAAGAAAGGGCACATCTGCTTCGAC *F GCCTCGTTCGAGACCGGCAACTTGGGCAGGGTGGATCTTATCTCGGAATTCGAGTACGATCTGTTCATCAGGCCGGACA *F CTTGCAACCCGCGACTTCGTATGTGGTTCAATTTCACCGTGGACAACGTGAAGGCCGACCAACGAGTGATCTTCAACAT *F AGTTAACATATCCAAGAGCGCGAATCTGTTTCGAAATGGGATGACACCGTTGGTAAAGAGTAGCAGTAGATCGAAGTGG *F CAAAGAATTCCCAGGGATCAAGTGAGTCATCGTCGAAAAGAACGAAGAAATACGAAGATATATCGAGAGATATCTTATC *F TTCCAAAAATTTTTATCCTAGGTTTTCTACTACAAATCGGCGCAACATCAAAACCATTACGTGCTCAGTTTCGCATTTT *F CTTTCGACCGCGAGGAGGACGTGTATCAATTCGCCCTGACGTATCCGTACTCGTACAGCCGTTATTTGGCGCATCTGGA *F CAACCTTTGCACCAGGTTAACGTACACGAGGAGAGAAACTATAGCCACGTCGATACAAAAGAGGAAGATCGAGTTGGTC *F ACGATAAGTTCGAATCTGGACGACGTTCAAGATCGTTCGAGAAAGGTGGTGGTTGTCCTCGCAAGGGTGTATCCA *F continuous ORF encodes *F LGNVNKIIIRPPGHSGKAKKGHICFDASFETGNLGRVDLISEFEYDLFIRPDTCNPRLRMWFNFTVDNVKADQRVIFNI *F VNISKSANLFRNGMTPLVKSSSRSKWQRIPRDQVSHRRKERRNTKIYREISYLPKIFILGFLLQIGATSKPLRAQFRIF *F FRPRGGRVSIRPDVSVLVQPLFGASGQPLHQVNVHEERNYSHVDTKEEDRVGHDKFESGRRSRSFEKGGGCPRKGVS *F Not part of any annotation, indeed is within the first 5.5kb intron of CG2246! *F And in opposite orientation. *F Best matches are a C. elegans and mouse genes, both to N-terminus, although *F they differ greatly in size (447 versus 137, but mouse may be incomplete) *F Try to reconstruct it. *F This is the entire intron sequence, in forward strand, from about *F 163912-169487 \- try using FGENESH at CGG website, and it predicts this gene, *F which is almost perfect. *F First, I probably would not have found the large first intron, but in fact is *F fairly well predicted and quite possibly right *F Second, I don't think the internal third phase X intron is needed, because it *F is open, so could add those aa to total *F |AE003774 *F aattcaaatgattagtacgagttagggtcaagagagtgcaaatcaaagccataacgaaacattaacttggattagggaa *F aagctacctgcgtttggacatgctgtcctagctgtagcttttggggttattggttatgttaatggggtggtgcatggtt *F ctctcggtggcataatcgttaaagtaacacacacgcagtcgttacggttcccacgttgcgatgtacgaccgtatcgatt *F agccccagtgaactgaatagcccccggtttttgcttgaacgcaattaatcctgccgctcctctcggttgcccagttgtg *F gaaactgctctctggctgcggaggaacccaaaaccacaggcgaaccgaagcccctagccggaaactactgcgaattatc *F gatcaaacaaaaaggcgacacacgtgagtggcgtggcaacgtcaaagtgccaacatgcgaatcacagttccggagtccg *F cagcccgaattccggattccaaggcagtcgtctgtgcggtgcggaggaagcggcgaggagcgaccaggagctgcccggg *F attagcagatagagatcgacctccagcgcccagacttcgcacgcgacttcaccgtcttgggagccccactcgagaaaag *F tgaaaggccaggaggcgttggctaattgaatttctatttttgatcctgcccaaggagcgaagccccaaagaaaagagaa *F gttgatgggttgaggcggcggaATGGGCGACTCAGgtaagcgaaatggtcataaatactcgcgataagtacgttgaggg *F aatcccctagcaaagcaatgtaaaaatacactaggagtttatgaagatgttattgaaaaatagttaaccatgaacaaaa *F gctagcaatctatacctcattccgaccaaaaatcacaacacccacggttagggtataaatgaagctatttaaaaacctt *F tcaagcctcgagacattcacctccggctttcaactcaggattggtttggcatgggcttgggattggggggccgacgcaa *F ccgcgaggcgactattcaattttagacatttgacgacttgagctgctcgaggctcagccaaaaggcgttggcaggtgca *F gtgagtgcaatggaaacttgcggcacgcatatgtggcacaaggaaaaggaaaggaccggcgggatgggatgccttgatt *F taagatagcagccgggtggcattaggaggaggattagaaggcgccccggccaagaaagttatgacaggccgggcagagg *F ccaaagcctcgagggattagggccactgcactcggattggcttggttgcattagaagaacgtagatttttgccaaagaa *F agacgcatcagcggataagagtcatcatgttgggaatagcctgcgatccacttcagcatacgagcatgtacgaacgcgg *F atcagatggtagtgaaattgatattaaacactcatcttgaacagattagaaactaagagatagtatactacgcagatca *F aaaaaagaatattggaaatatttgctcttatcaatcagtttcttatttcctaaactagaggcatgccgcttaaaatctt *F tttggctcaattcatagtttttatacttctaatggatattcctttgcagATAGCGAAGACAGCGACGGAGAAGGCGGTC *F TGGGCAACGTTTCCCGGGTTATCATCCGTCCGCCGGGTCAAAGTGGCAAGGCCAAGAGGGGCCATCTCTGCTTTGATGC *F GGCCTTCGAAACAGGAAACCTGGGAAAAGCGGAGCTGGTGGGCGAATTCGAGTACGATTTGTTCCTTAGACCGGATACG *F TGTAATCCTCGCTTCCGTTTCTGGTTTAACTTCACCGTGGACAACGTAAAGCAGGATCAGCGAGTGCTCTTTCACATTG *F TTAACATCAGCAAGAGCAGGAATCTCTTCTCCTCGGGACTGACTCCCTTGGTGAAGAGCTCCAGTCGACCCAAGTGGCA *F GAGGCTGTCCAAGCGGCAGGTGTTCTTCTACCGATCGGCCATGCACCAGGGTCACTATGTCTTGAGCTTCGCCTTTATC *F TTCGACAAGGAAGAGGATGTCTACCAGTTTGCGTTGGCCTGGCCTTATAGCTATTCGCGTCTGCAGTCCTATTTGAATG *F TGATTGATGCCCGGCAAGGATCGGA *F M G D S *F \---------------------------1------------------------------------------------1- *F -----------------------------------------------1------------------------------- *F -----------------1------------------------------------------------1------------ *F ------------------------------------1------------------------------------------ *F ------1------------------------------------------------1----------------------- *F -------------------------1------------------------------------------------1---- *F --------------------------------------------1---------------------------------- *F --------------1------------------------------------------------1--------------- *F ---------------------------------1--------------------------------------------- *F ---1------------------------------------------------1-------------------------- *F ----------------------1------------------------------------------------1------- *F ---------------D S E D S D G E G G L G N V S R V I I R P *F P G Q S G K A K R G H L C F D A A F E T G N L G K A *F E L V G E F E Y D L F L R P D T C N P R F R F W F N *F F T V D N V K Q D Q R V L F H I V N I S K S R N L F *F S S G L T P L V K S S S R P K W Q R L S K R Q V F F *F Y R S A M H Q G H Y V L S F A F I F D K E E D V Y Q *F F A L A W P Y S Y S R L Q S Y L N V I D A R Q G S D *F Fly protein *F MGDSDSEDSDGEGGLGNVSRVIIRPPGQSGKAKRGHLCFDAAFETGNLGKAELVGEFEYDLFLRPDTCNPRFRFWFNFT *F VDNVKQDQRVLFHIVNISKSRNLFSSGLTPLVKSSSRPKWQRLSKRQVFFYRSAMHQGHYVLSFAFIFDKEEDVYQFAL *F AWPYSYSRLQSYLNVIDARQGSDKRFTRCVLVKSLQNRNVDLLTIDHVTAKQRSTNRLDRSFIRVIVVLCRTHSSEAPA *F SHVCQGLIEFLVGNHPIAAVLRDNFVFKIVPMVNPDGVFLGNNRCNLMGQDMNRNWHIGSEFTQPELHAVKGMLKELDN *F SDvsrgietdligiifvcsynisfqTYQIDFVIDLHANSSMHGCFIYGNTYEDVYRYERHLVFPRLFASNAQDYVADHT *F MFNADERKAGSMRRFSCERLSDTVNAYTLEVSMAGHYLKDGKTISLYNEDGYYRVGRNLARTLLQYYRFINILPMPIVT *F EVRSKRRGRNRHAHHSRSRSKTRYEVKPRPKTPRCHAPIAYTNLSICYDSGGGGGSSDEGGFSPARPLAPGSSCFSGYR *F NYRRAATASCSAHPGHDQYSPFALGALKTGSDHGGGVGGSKGKRSAAVTIEVPLPVNVPPKPYLSIIDLNQLTRGSLKL *F KSNSFDAADRRZ *F Bee EST *F LGNVNKIIIRPPGHSGKAKKGHICFDASFETGNLGRVDLISEFEYDLFIRPDTCNPRLRMWFNFTVDNVKADQRVIFNI *F VNISKSANLFRNGMTPLVKSSSRSKWQRI-not sure why no match after *F this?-PRDQVSHRRKERRNTKIYREISYLPKIFILGFLLQIGATSKPLRAQFRIFFRPRGGRVSIRPDVSVLVQPLFG *F ASGQPLHQVNVHEERNYSHVDTKEEDRVGHDKFESGRRSRSFEKGGGCPRKGVS *F No Drosophila ESTs for this region *F Great BLASTP matches to both C. elegans and human proteins of comparable size *F in GenBank *F \**********A. mellifera BB270028B10A5.F *F AGAACCCTTAGATCTTATAAGATTGAGTCTTGATGAAAGAATATACGTTAAAATGAGAAACGAGAGAGAATTAAGGGGA *F CGATTACATGCTTACGACCAACATTTGAATATGGTGTTGGGTGAAGCAGAGGAAACCGTAACCACAGTAGAAATTGATG *F AAGAAACATACGAAGAAGTGTATCGTACTACTAAAAGGAATATTTCTATGCTTTTTGTTCGTGGCGACGGTGTGATTTT *F GGTTTCACCACCGAGCATGAGAGCACCGATATAAAAAATTTCTATTTACATTACAATCTGTCTAAAAATTAAGAAACAT *F ATAAGTAATATATAATATTACGTCGATACAATTTTATCAACTTTTGTAACGAGCATTTAACAAGTGTGTCGACAAAAAT *F CTAACGACAAACCAACCATAGATATTTAATTATAGATGAGAAGAATCTGTAACGATAGGAATATAAAAAATTGTAATCC *F AAATATGGAAAAAAGAAAACAAATCAAGATGTGGTACACTATGTTAAAATGATATATTAATTAAAAGTTTATAAATAAT *F TATAAAAAAAAAAAAAAAAAAAGCAAC *F This EST, plus genomic matches, plus human match, plus a Drosophila EST all *F indicate that the N-terminus of CG5926 is missing *F \**********A. mellifera BB270029A10G1.F *F TTTTTTTTTAAATATGGAATATATATACCGTTTACCATTTTTAGCATTAGAAGTACCTAACTTAAAATTAAAGAAGCCA *F TCATGGTTTGTGAAACCTAGTGCTATGATAGTTTTTTCATTTATACTTTTATCATATTTTCTAGTAACTGGAGGTATAA *F TATATGATGTAATTGTGGAACCACCTAGTGTAGGCTCAACAACAGATGAACATGGCCATACAAGACCTGGAGCATTTAT *F GCCGTATCGAGTAAATGGGCAATATATTATGGAAGGATTGGCATCTAGTTTCCTTTTTACATTAGGTGGAATTGGTTTT *F ATAGTATTAGATCAAACACATAATCCATCAACACCTAAGCTTAATAGAATTCTTTTAATATGTGTTGGATTTATTAGTG *F TTATTGTCTCATTTATTACCTGTTGGGTTTTTATGAGAATGAAACTACCGGGATATCTGCAATCATAAATTTAATAGAT *F TTAAAATAATATGGCAAATTGAGCTTAAAACTCAATATTTTGTACTCATCAAAACTATTACATTTTGTATTAAATATGT *F GGATTAGAAATGTGATTTATAAATAATATTATAAAAAAATTCTGTCAAATATATTTAAAAAAAAATAAATTCTTATTTT *F TATTTAAAAAAAAAAAAAAAAAAAGCAAC *F This and vertebrate matches, B. mori EST, and Drosophila EST, show there is an *F intron in the annotation of CG9662 that is open and in frame and encodes *F needed aa. *F \**********A. mellifera BB270029A20C10.F *F TATGGTCGCACGAAACCGCTCATTTCCAGGACAATGATGAAGAACATTCTTGGCCAAGCTATCTATCAGTTGACTGTAA *F TTTTTATGCTTCTTTTCGTTGGTGATAAGATGCTCGACATCGAAACAGGCCGAGGAGTTGCGCAGGCTGGTGGCGGTCC *F AACGCAACACTTTACTATTATCTTTAATACATTCGTCATGATGACACTTTTCAACGAATTTAACGCTAGAAAAATCCAT *F GGTCAGCGTAATGTCTTCCAAGGAATATTCACCAATCCCATCTTTTACACTATCTGGATCGTGACATGTCTATCGCAGG *F TAGTTATCATACAATATGGTAAAATGGCGTTCAGCACGAAAGCTCTCACATTAGAACAATGGATGTGGTGCCTATTCTT *F CGGAGTCGGTACTCTATTGTGGGGCCAAGTAATTACAACTATTCCTACGCGCAAGATTCCTAAAATCCTTTCATCCGCG *F TAGTGAACGCATTTCGGCAGGGCCTAGACGCACGCTACACGAGCGAGCACAGCAGCACTACATTGGCGGAGGTACTGAG *F AAAACAGTCGTCCTTAAGCAAGCGGCTCTCGCAACGAGCAGCATTGAATACGCCGATAACAACCCAGACGAACTGACCA *F TACCCGAGATAGATGTGGAAAGATTGTCAAGTCACAGTCATACAGAGACCGCTGTTTAGAATGGGAGAAGATGGCGGGT *F CAGCA *F This and vertebrate and nematode and Drosophila EST indicate there is an *F intron boundary problem in CG2165 *F \**********A. mellifera BB270030A10C8.F *F GAGTGGACAATTTCTCAGAGGTGCCTTTAGTAGTGAATCAGATTACTGTACACGATAATTTGCAATGGACAGTCAGACC *F ACATTCCTCTGTACCATATGCATTAGACGAACCCATACAATCTGCTGGTTTGGTTCTAACTGCACCAGGAGGTGTGACA *F GCTACATATGATTTAAATATACTTGGAGAGGGCAGAGTTCTGACTTATGAAAATTTTATTTATATTGCTTTTACAGGAA *F CCTTTAAAAATGATTGTGATCTGGGAACAGGAGAAAGTGGTTTAGATCCTTTGGATGTTGAAACACAGAGATTAGTTTT *F AGAAGCTGGTCCTGGTGGTTGGGTTGCTTTGCGCAGAAAACAATATGGAGCTAGATCTCAATTATGGAGAATGACTGGA *F GATGGTCAATTACAACATGAGGGATCTAGTCCACCTAGAGATAAACATTCAAAAGTTCCAGAGACAGTATTAGTATTAG *F ATATAGCAGGCACAGCACCTCAACCATTCACTTATTGTGCTCTTGCCTTAAGAAAGCCTGATCCCCGAAGGAGATCTAC *F ACAAACTTGGCGATTCACAGATGATGGACGATTGTGTTGTGCACATAAAAATATGTGTGTGCAATCGAAAGATGGATTC *F ATGGGATTACATGAAGGGAGTGAAGCAGTATTAGGTCCTCAACACATACTAATCCTCCTCCAATTGAACAGCGTGTTGG *F TAGACAA *F The N-terminal region of the translation of this EST has clear vertebrate and *F Drosophila genomic matches, indicating that the annotation of CG11003 needs a *F change. *F \**********A. mellifera BB270032A10D4.F *F TGCAACCTTTTTTGCGAAAAATGTTGGAATTACAAATATTTCAACAATTTATAGAAGAAAGACTGAATATGCTTAATTC *F AGGACTTGGTTTTTCTGATGAATTTGAAATGGAGGCTTGCAGTTATTCTGCTAAATCTGGTAGCAAATTTATGCAGCAA *F TATCGAGAATGGACTTATACTATGCGAAAAGAAAGCTCTGCATTTTTCCGCAGTGTAAAAGACAAGGCCAATCCAGCTG *F TCAAATATGCAGTTAAATCAGTAAAAGATAAAGGAAAAGATATGAAAACGGTATATAAAGGATTAAAATGGAAAGGACG *F ATCAAATAGAAGCGATACAAGTTTGAGATTCCATCAACCAAGATCAGCACCTAGTTCACCTACATCGGATCGAAGGCCT *F ATCGATTTTTCATCACCTCCAAAATCTCCAAATGGTTTTACTGCTACTACTAGTTATAGAAAAGATCTTCGAATACGTA *F ATAGTAATTTCACCGATTCAAGCAGAAAACAATATTCACCATTAAGTCCTAGTTCACCAGAAGAATCTGATTTTCCACC *F AGAAAGAGTAAATATTGATTTGATGCAAGAACTCCGTCATGTAATATTTCCGAACACACCTCCTGTTGATAGAACAGTT *F TCTCCTGAAGTGCCAGATTTAATTAGATTAGATTCGACAACAAGTACTGAAGATTTCGATCCACTACTTTCTAAAT *F This and a new Drosophila testes cDNA, and vertebrate matches, show that *F CG18659 needs a C-terminus *F \**********A. mellifera Contig1366 *F GAATTTCTTACCACTTTTAAAATCGACATCTGTCATTTCAAATGGAATGCAACCAGTGACCGGAAGGGGTATTGTAGAA *F AAAATAAAAATTATTACTAAATCTGACGTCAACCAGATTCTCGTTCAAAGTTCGCAAGAATCTCTTATTACAGGAGCTC *F TACGAGTGAGTTCAGGAATTGGAGCAAGTTTATTAGCAACGCAGAGGAGGTTAGCACATACTGATATTCAGTGGCCAGA *F TTTTAGTGATTATCGTCATGAAGCTGTACAAGATCCAAGAAGTAAAAGCAAAGAAAATTCAAGTAGCCGTAAATCATTT *F GCATATGTTATGACAGCTGCAAGTGGAATTACCGGTGCTTATATAGCAAAATCAGCCATACATGATTTAGTAGCTACAT *F TTAGTGCTTCAGCTGATGTACTTGCATTGGCAAAAATTGAAATAAAACTTGATGCTATTCCTGAAGGAAAAAGTGCTGT *F CTTTAAATGGCGAGGAAAACCTATATTTGTACGACACAGGTCAAAAAAAGAAATTGAAAAAGAGGCAGCAGTTGATATT *F AAGATTCTTAGAGATCCACAAGTGGATTTAGATCGTGTAAAGCAGCCACAATGGTTGATTGTTTTGGGTGTATGTACAC *F ATTTAGGATGTGTACCAATTGCAAATGCAGGTGATTTTGGTGGTTATTATTGTCCTTGCCATGGATCTCATTATGATGC *F TAGTGGCAGGATTAGGAAAGGACCAGCTCCATTAAATTTGGAAGTACCACCTTATGATTTTATCGACGATAATACAGTA *F GTTATCGGTTAATGTATTAATATATGTAGTGAAATGATAAATGTAATTTATATCAAATTGTTTTACACAAATTCGATTT *F TTATTTGTAAATTTTTTCCTAGAACCATCACATGTTGAAATGTTAAACTACAGTGAAATATAAATAAAAAACTTAAGTT *F AATTTTAAAAAAAAAAAAAAAAAGCAAC *F This and vertebrate matches show there is additional complexity to CG7361 \- *F lots of Drosophila EST? *F \**********A. mellifera Contig1440 *F GTCAATGTTAATTTAAATCGAAGTTTTTAATATTTTTGTGGCGTGGCTCGAATAAACTCTTAATATTCGTATATGTAAA *F TCAGAAAATTATGTGGAAGGCAGTGTTGTTGATCGCAATCCTGTCCGCTGCAACGAATAAAAGTGTTGCAAACGACTGC *F GTGCCACGAAGTTTCGGGACAAATAATATCGTATGCGTTTGCAACTCGACTTACTGCGACAGCACACCGGAACCGAAGC *F CGAGCAGTCCAGAAAAAGGTACTTTTCACTGGTACGTATCGAGCAGAGATGGCCTCAGGCTGAGCTTGTCAAAAGGACA *F AATGGGCCGTTGTCAAAACGATGGATCTTTAACCCTGAACATCGATACCTCGAAAAGATATCAAACGATCCTCGGTTTT *F GGTGGCGCTTTTACCGACTCGGCGGGAATGAATATCAAGAATCTGAGCGAGGCTACTCAGGATCAATTAATCAGAGCAT *F ATTTCGATCCGAAAGATGGAAGTAGATACACGTTAGGCCGTATACCAATAGGAGGAACAGACTTCTCTACGAGAGCCTA *F TACATTGGACGATTACGATGACGATGCGACGTTGCAACATTTCGCGCTTGCTCCTGAAGATGTCGAGTATAAGATACCG *F TATGCGAGGAAAGCTGTCGAATTGAATCCCGATTTAAGATTCTTTAGCGCCGCGTGGTCGGCGCCGACATGGATGAAAA *F CTAATCACAAAATCAATGGATTTGGTTTCTTGAAGACCGAATATTATCAAACTTTTGCCAATTACATATTGAAATTCAT *F AGAGGAATATAAAAAGAATGGAGTAGATATATGGGGTGTTTCAACTGGAAACGAACCATTCGATGCCTATATTCCTTTT *F GAACGTCTTAACAGTATGGGATGGACACCAGAGCTGGTTGGCGATTGGATCGCTAACAACTTGGGCCCAACTTTGGCAA *F ATTCCGAATACAACGCCACACATATTTTCGTTTTGGACGATCAAAGACTAGGATTACCTTGGTTCGTTAACGAAATCTT *F TAAAAATGAAATTGCGAGAAATTATGTTTACGGCATAGCCGTACATTGGTACGCGGATATATTGATTCCACCGGTAGTA *F TTAGATCAAACGCACAATAATTTTCCTGACAAAAATCTGTTGATGACCGAAGCATGTGAAGGATCTTTTCCATTGGAAA *F AAAAGGTTGTGTTGGGATCATGGGAAAGAGGAAAAAGATATATATTAAGTATAACGCAGTATATGAATCATTGGGGAGT *F TGGATGGGTGGATTGGAACATAGCTTTGAACAAAGATGGTGGACCAACCTATATCAATAATAACGTCGACTCGCCCATT *F ATTGTAAATCCGGAAAATGATGAATTTTATAAACAGCCGATGTATTACGCTCTTAAGCACTACAGCAGATTCGTCGACA *F GAGGATCGGTCAGGATTTTCATCACCGACACGATTGAAATTAAGGCCGCAGCCTTTATAACGCCCTCGAACGAAATTGT *F GGTTGTTGCGTATAACGACAATAATGAAAAAACAAATGTGGTTCTGAACGATGTGACAATTGAAGATTATATTTGTTTG *F GAATTACCCCCACATTCTATGAATACTGTAATTTACAACAAATAGAATACAACATATGAAATGAAAGATCATATGAATG *F AAAAGCAACCGATGAAGTATATTACAAATTCTATCATGTTAAA *F This encodes a full-length ~500aa protein with ful-length matches to various *F vertebrate and nematode proteins, and indicates that CG10299 may need to be *F split into two or three proteins *F \**********A. mellifera Contig1578 *F CGGACCGATGGTCGGCCTACGGTATCCCCACCCAAGTCCCGGCGGGGTACGGTTCCCCGGCAAAGCAACCGATTTCAGT *F AGCAGGACAACAACAGAATGCAGCGTCTACTGGTAAAGTCCTGACTGGAGATTTGGATAGCAGTCTTGCTAGTCTTGCT *F CAAAATTTGACCATCAACAAAAGTGCTCAGCAACAAGTCAAAGGTATGCAATGGAATTCGCCTAAAAATGCTGCCAAAA *F CTGGTGGCTCAGCTGGAGGATGGACACCGCAACCTATGGCAGCTACAACTGGCGCTGGATATCGTCCAATGGGAATGCA *F AGGTGTGCCAATAGGCATGCAAAGTATGCAAGGCATAAGACCTATGATGAGCACAATATCTGGTGGTCCTGGTAACATG *F ATGGTCACAGGAGGAGCTGCACCAATGATGATGTCTAGTTCAAATTCGATGATGGGTACTAACCTTCAACAACAACCAC *F AGCAGCAACCACAGCAGAATATTGCGCAACCACAAAATAATCAAGTTCAACTTGATCCATTTGGTGCCCTGTGAACAAA *F TTAGGTATCTATTTGATATCTCCTTGTGAAAAAAAAATTATCAATAATAAAATATGGATTATGATTTTAATTAAGTAAA *F ACATTATTTAATTGAATACTTAAAATATTTATAAATTTTGCCTCTTTACATTCCCATTTTTTGAATTTTATGAATTTTC *F AGTTTGCGAATGAACAGAAAAATGTATTGATTTGGTAATGTTATTTTGAAAAATTTTTTTTCAGAAGTAATTATATATA *F ACAGAATATAATTACTTCTGAAAAAAAAAGCAAC *F This EST, plus vertebrate matches, plus an A. gambiae EST, indicate that there *F are two exons missing near the C-terminus of CG2520 *F \**********A. mellifera Contig1667 *F TTAAAAAAAATATTTATATTTTCCTATTATATTTGGTCAAATAAAACACAATTCTTTTGACAAAGAAAAATTATCAGAA *F TTGTTTCATTGAAAGAAGTTTGCTTTGCAATTTGTAGATATTTCAATTAAGGGCAAGATTATTGGATTTTAATAAAATT *F TTAAAAATGGAGGTGGATTATAGTAGCAATTGTGATGTTAAAATTCCAGAATGTAAAAAATTAGCAAGTGAAGGAAAGT *F TACATGATGCTTTGGACCAATTACTAGCATTAGAAAAACTAGCACGAACAAGTGCAGATGTGGCATCTACATCTCGAAT *F TCTTGTTGCTATTGTTCAAATTTGTCTAGAAGCAAAGAATTGGGCAGTATTAAATGAACACATAGTATTGTTGTCCAAA *F AGACGTTCTCAATTAAAACGAGCTGTTACAGCAATGGTTCAAGAATGTTGTACTTATGTAGATAAAATGCCTGATAAAG *F AAACCAAAATTAAATTGATAGAAACATTACGTACTGTAACAGAAGGAAAGATATATGTAGAAGTTGAAAGAGCAAGACT *F TACTCATCGTTTGGCAAAAATCAAAGAAGAAGATGGAGATATTTCGGGTGCAGCAGCTGTTATGCTTGAATTACAAGTT *F GAAACATATGGTAGTATGTCACGTTTAGAAAAAGCATCTCTTATTCTAGAAGCAATGCGATTATGTTTAGCaAAAAAAG *F ATTTTATGCGCACTCAAATAATAGCTAAGAAAATTAATGTTAAATTTTTTAATGATGAAAATGATGAAGAAACACAATC *F TCTTAAATTGAAATATTATGATCTAATGATGGAATTGGCTCGTCATGAAGGTTGGCATTTAGAATTATGTAGACATAAT *F CGAGCAGTATTGGAAACTCCAGCAGTTAGAGATGATCCTGAAAAAAGACATGTTGCACTTTCACGAGCTGTTCTGTATC *F TCGTACTTGCACCACATGAACCAGAACAAGCTGATTTGACCCATAGGTTGCTTTCTGATAAACTTCTTGATGAGATACC *F AACATACAAGGAATTATTACGACTTTTTGTAAATCCAGAACTAATAAAATGGTCAGGACTTTGCGAAATTTACGAAAGA *F GATCTTAAAGCTACAGAAGTTTTTAGTCTATGGACTGAAGAAGGACGTAAACGATGGGCCGATCTTCGAAATCGTGTTG *F TCGAACATAATATCAGAATTATGGCAAAATATTACACAAAAATTACATTGACTCGCATGGCTGAATTATTGGATTTACC *F AGTTGAAGAAACTGAAGCATGTCTATGCAGTTTAGTAGAAACTGGTGTGATAAATGCCCGTACGGATCGTCCAGCCGGT *F GTGGTTCGTTTTACAGGAACCCAAGAGCCGGCTGCTCTTTTGGACGCATGGGCTGCATCTTTATCAAAATTAATGAGTC *F TTGTCAATCATACAACTCATCTTATTCATCAAGAAGAAATGTTGGCTGTAGCTCAATCCTGAAAGATAAATTTTCACTT *F TTTCTTTTTCCCTATTTTTTTTTCTCCTTTCTTTCTTCTCTTTGGATCAAAACTTGGTTAATTTTCTTCTTTATATAAT *F CCTTTTACTTTTTTAAAAGTTATTTCCATAAAATGACTGCACATAAATAATTGTACACTTTCTATAAAACTTTAAAAAA *F CTTTAAAAAAAACATTTAA *F This long contig indicates that there might be an unspliced intron in the *F Drosophila gene CG3294; it's a proteasomal subunit and the honeybee sequence *F aligns well with all others, but Drosophila has a large insertion. *F \**********A. mellifera Contig1674 *F GTATACTTGTTGACCATCCCCGGCCTTCGACGTGCACCTGTCAGCGTCATGTATTCGCGTTCTCGAATGCGACCACGCC *F GACTCTTCTCCACGATGCCTTTCGTGTTGTAAATAATACTGTGAAAAGAAACCTCAGACCGAAGATGGGCAAGCTTTTG *F AGCCTTCTGGCTCGAGATGAGTCTACCTGTTGCACCCCTCAAAAGTACGACGTCTTTTTGGATTTCGAAAATGCACAAC *F CTTCCGATATAGAACGGGAGACCTTTGAAGCGGTGCAAAGAGTTTTGAAAAATTCAGAATCTATTTTAGAGGAGATTCA *F ATGCTACAAAGGTGCCGGAAAAGAAATCAGGGAGGCGATTTCGGCTCCCACGGAAGAGTGTCAACGAAAAGCTTACCTG *F ACTGTTGCACCTCTAGTCGCCAAGCTGAAAAGATTCTATGAATTTTCATTGGAACTTGAGAAGGTAGTACCAAAAATCT *F TAGGCCAACTGTGCTCCGGTAATCTCTCCCCGACCCAACATCTCGAGACTCAACAGGCATTGGTGAAACAGCTGGCGGA *F AATTCTGGAATTCGTCTTGAAATTCGACGAGCACAAGATGAAGACACCCGCTATTCAAAATGATTTTAGTTATTACAGA *F AGAACGTTGACCAGAGCATCTCTGGCGCGACAAGAAAGCGCTGAAAAGGACCTCGTGGTCGGGAACGAGCTCGCCAACC *F GAATGTCCTTGTTCTACGCCCACGCGACACCCATGCTTCGTGTTCTGAGTCACGCGACCATTACTTTCTTGATGGACAA *F CGAAGATGTAGCACGTGAAAATATTACTGAAACTCTTGGCACCATGGCCAAAGTTTGTCTGCGCATGTTAGAAAATCCG *F AATTTATTGGCGCAATTCCAACGAGAAGAAACTCAACTCTTTGTTCTGAGAGTGATGGTAGGGTTAGTGATCCTTTATG *F ATCATGTTCATCCCCAAGGTGCCTTTGTTAAGGGTTCGAATGTCGATGTTAAAGGCTGTGTAAAGCTATTGAAGGATCA *F ACCACCTTGCAAAAGCGAGGGTCTTTTGAATGCTCTTCGCTACACCACCAAGCACCTGAACGAGGAGAACACACCGAAG *F AACATTAAGAACCTTCTAGCAGCATGATTACCAAAGCAGCGCGGATGCTGCATCAGAAGCTAAGACAAATTGAACGAAT *F GACATCCATTCAAATTTTGTGTGTACGAGATCGTCGAAGCGACTTTATATGACACCAAACAAGCAATCTCCTGTTTCTG *F ATCGTGGCTGGGTGGTGGACCATTAAAAAAAAAAAAACGTGTCTTTCGTAATAGAGTCTATAGCTGCCCGAAAACTGCC *F GACTTCTACATACCTTCTGACGAAAAAAAGAAATTAAAAAAAAATAAATAAATAAATAAATAAATAAAAAAAACAAAAA *F AAAGTAAAAAAGAAAAAAAAAAAAAAAAAAAGCAACGC *F This contig, plus vertebrate matches, show that CG6487 and CG6491 should *F probably be fused. There's a B. mori EST that agrees, but amazingly no *F Drosophila ESTs at all. *F \**********A. mellifera Contig1686 *F GATAAAAAGAGTATGCAAGCTTTTTTAAAAACTGGTAAATTAGGTCCTGGCGAGTTCAAAAAAGTTTCGAATTCACGTT *F CAAAAGAAGAACGTAGTGGTCCTGCACCGCCATGGGTTGAGAAATATCGTCCAAAGAACGTAGAAGATGTTGTTGAACA *F AACAGAAGTAGTAGAGGTATTACGACAATGTTTAAAAGGAGGTGATTTTCCAAATTTATTATTTTATGGTCCACCTGGA *F ACTGGTAAAACAAGTACTATATTAGCTGCAGCTAGACAATTATTTGGTAGTCTTTATAAAGAAAGAGTATTAGAATTAA *F ATGCTTCTGATGAACGGGGTATTCAAGTTGTAAGAGAAAAAATCAAATCTTTTGCACAACTTACAGCAGGTGGTATGAG *F AGATGATGGAAAAAGTTGCCCTCCTTTTAAAATTATTGTCTTAGATGAAGCAGATAGTATGACTGGTGCTGCACAAGCT *F GCACTTCGTCGTACTATGGAGAAAGAATCTCATAGTACTAGATTTTGTTTGATTTGTAATTATGTATCAAGAATCATAG *F AACCTTTGACTTCTCGTTGTACAAAATTCAGATTTAAACCATTAGGAGAAAATAAAATTATTGAGAGATTAGAATATAT *F ATGTAAAGAGG *F This contig and human matches show that CG8142 needs an N-terminus and it is *F available in the genomic sequence, with an N-terminal exon and intron. There *F are embryon Drosophila ESTs showing this. *F \**********A. mellifera Contig1749 *F TCAAAATGGGAAATTGTTTGAAACGCGCTGGAAGCGGTCAACAGGACAATACCACTTTGCTGAGTAACAATCCTGATCC *F TCCTACATTGACTAGCGGTTCTTTACAAGAAGGCCTTGGACCTCCGATACCTAACAATGAGGCTGTAACCTTTTCTTAT *F GCACCAGTTTTTACAAGGGAACTTCATCTTCAACAAATTGGCATTGGTGTTAATCTAGGACCAGGTAGTGAAGAAGAAC *F AACAAGTTAGAATAGCAAAACGCATAGGACTTATTCAACATTTGCCTATGAGAGAATATGATGGGACTAAAAAAGGAGA *F ATGCGTGATATGTATGATGGAGCTGCAGGTGGGAGAGGAAGTGCGTTATTTACCCTGTATGCATACTTATCATGCAGTA *F TGTATCGACGATTGGTTGCTGCGTTCTTTGACTTGTCCATCGTGCATGGAGCCTGTAGATGCAGCATTGATTAGTTCAT *F ATCATCCAACCACTTAACACCAAGGAGATGGAGAATGAAATAGCTAATCGGTAATCAAACTATATTATCGCGATGAAAC *F AGAGAAATTGGAAAAGATTATTAATTTTATTATCATATATAATGGCTTCTAAATAGCAAAAGGGTATTTTCTTCTTGTT *F ATGGCTAAATTTGCCATTGTATTCAAATATATCTGTTCGCATGCTAATAAT *F Encodes full-length protein, by vertebrate and other matches, which is *F unannotated in the Drosophila genome *F MGNCLKRAGSGQQDNTTLLSNNPDPPTLTSGSLQEGLGPPIPNNEAVTFSYAPVFTRELHLQQIGIGVNLGPGSEEEQQ *F VRIAKRIGLIQHLPMREYDGTKKGECVICMMELQVGEEVRYLPCMHTYHAVCIDDWLLRSLTCPSCMEPVDAALISSYH *F PTT *F There's even a head cDNA for it. *F AE003844 *F TAACACCATCTTCTAACAATCGTCAACTTTCCGATGAAAATCAAGTGAAAATTGCAAAGCGAATTGGATTAATGCAGTA *F CTTGCCAATAGGAACATACGACGGGAGCTCAAAGAAAGCACGAGAATGTGTTATCTGCATGGCTGAATTTTGTGTTAAT *F GAAGCCGTACGTTATCTACCTTGCATGCATATTTATCATGTTAATTGTATAGATGATTGGTTGTTGAGAAGTCTAACTT *F GTCCCAGTTGTTTAGAGCCTGTTGATGCTGCCTTGCTGACGAGTTATGAATCGACATAGCGTTATAAAAACATTAGCTT *F ACAATTTTGCTGCTGTAATGTGTTTTGGGATAACAAAACCTTTG *F GH26713.5prime *F GAATAAATCAGGCTTTATTAAATCGAATCTAGTCTAATTTCAAAGAAAGTCACATTTAATGTTTTTTTTTTTTTAAATC *F AACTAACTAAATTGTTTCTGTTTATTATGAAAGTTGTGTATACATATGTGCATTTTATATACATGCATGCGTACTTATT *F AATTTAAGATTTCTTGGGGATTGGTACTAATTGGTACTGTATATTTAAATCTTCGAAAAACGCATGAAATGGGTAATTG *F CTTAAAAATTAGCACTTCAGATGACATTTCACTTTTACGCGGCAATGACAGTCAAATCAGCGGGACACAGCCAGTGTAT *F CATCAGGGAGAGCATTATCAACGAGAATTGTACCCTTCCACGTCGTCTTCGACAACGCTAACACCATCTTCTAACAATC *F GTCAACTTTCCGATGAAAATCAAGTGAAAATTGCAAAGCGAATTGGATTAATGCAGTACTTGCCAATAGGAACATACGA *F CGGGAGCTCAAAGAAAGCACGAGAATGTGTTATCTGCATGGCTGAATTTTGTGTTAATGAAGCCGTACGTTATCTACCT *F TGCATGCATATTTATCATGTTAATTGTATAGATGATTGGTTGTTGAGAAGTCTAACTTGTCCCAGTTGTTTAGAGCCTG *F TTGATGCT *F M G N C L K I S T S D D I S L L R G N D S Q I *F S G T Q P V Y H Q G E H Y Q R E L Y P S T S S S T T *F L T P S S N N R Q L S D E N Q V K I A K R I G L M Q *F Y L P I G T Y D G S S K K A R E C V I C M A E F C V *F N E A V R Y L P C M H I Y H V N C I D D W L L R S L *F T C P S C L E P V D A A L L T S Y E S T \* *F Drosophila protein is *F MGNCLKISTSDDISLLRGNDSQISGTQPVYHQGEHYQRELYPSTSSSTTLTPSSNNRQLSDENQVKIAKRIGLMQYLPI *F GTYDGSSKKARECVICMAEFCVNEAVRYLPCMHIYHVNCIDDWLLRSLTCPSCLEPVDAALLTSYEST *F \**********A. mellifera Contig2388 *F TGGGATGTGAAAACACTTTCGGAATGCTGTAGGACTGATCATGGTTATACACCAGATTCTCGTGCTATTCGTTTTCTAT *F TTGAAGTTATGTCAAAATATAATAGTGAAGAACAAAGGCAGTTCGTTCAATTTGTTACAGGTTCACCTCGATTACCAGT *F AGGAGGTTTCAAGAGTTTAACACCGCCGTTAACAATAGTGCGTAAAACGTTCGATCCATCTATGAAAACAGACGATTTC *F TTACCATCCGTAATGACTTGTGTTAATTACTTAAAACTGCCTGATTATACAACATTAGAAATAATGCGGGAAAAGTTGC *F GAATAGCTGCACAAGAAGGACAACATTCGTTCCACCTTTCCTAGAAACGGATGGAAAACCGGCGCGCCATTTGCTCTTT *F TGCTATCGTATTGTCCAGGTTGAAAAAATTCTTCAAACACCATTTAAAATATTTAAAAAAGTAACTGCGCGCGCGTTTT *F CTTCAACTCAGTAATCGTATATTTGCACTTTATATGAGCTTTTTCGAAAATATCTTTTTCTTGATTTTAGTGAGAATTC *F ATTTAAATAAATTTTAATTTGATGTTTATCTTTTTACTTATAAATATGGATTGTGATATATTTTCTCAAAAAAGTGCAA *F AAATGACATTAGCTCTATACTATGAGTTTCCTCTTGTGGTATTATGAACCAGGTAGAATGGGAGCAGCCTTGTTCTAGG *F AAACTTATCTTTGATGTGTTACAATAATTAATAAAAGTTGTAAATATAGTTTAATATGCGCAAGTCTATATAAATACTT *F TATATAATAGTAACAAAAAAAAATGATATTGCTACGAAACTCTATACTAATTAAATATAATTTAATCTGAGAGGACGTC *F TATTAGACATAAGTAAATTTTATATCAGTGGTGCATTTAGCATCGCCATAAATCGCCAATTTCATGCCTCACTGCAGAT *F TGTAATGAAATCACAAGTCAAAATTGATCAAAAAAGAAAAAAAATACGCGCTAATAACGTAGCTCATGTTTCATCATCA *F TAAAAATATAATAACTGCGAATATAATTGCGTGTTTACGATTATCGTTTTAATTTCTTAATCGATCTCTTTTGTTCGCT *F GCTTAGTTCTCAGTATGCGAGCACTGCGCGAATTAACGTGCAAAACTCTTATATTTAAGTTTATTCAATGTATCACTAA *F TTAAACTTATTTGATATTTTTATTC *F This EST and mammlian matches indicate that CG17735 needs a C-terminus. Seems *F to be indicated as a transcribed region, but not in translation? *F \**********A. mellifera Contig2672 *F GGTATTATGATGGTTGTACGCGATGCCTGATCCCGTAACGCTGAGCAACAATGGTGACGTAGAGCTGTTCATCCTGGCG *F GTCGTATGATCCTCGGCGGTCTTGTTCGGCAAATTGCCCTTGCTGGCGTTCAGCGCACTGTTTGTCGGGTCTTCGTCAA *F TGTATGTGAAGTCAGAAAGTGGTCGGGGCCCAGTGTGGCTGCGTGAACTGCGCATACTGTGTAAGCTGTGTAAACTATG *F GACAGAGCGACGCTCCTCCAAATCTTCTAGAGTCGACTTGAACGCTCTGATTACTCGAAGCTGTGTCTGTAGTCGTGTT *F AGACCACGGATCCATAGAATTTGTCCTGCGCGCGGCTTTTTATCCGAGTCAGGGTCGAATTTCTCATCTCCTAGATTGA *F TCGCACTGATATCATCCGGCTGGCCGCGGCCCCATGAAAGGATTTTAGGAATCTTGCGCGTAGGAATAGTTGTAATTAC *F TTGGCCCCACAATAGAGTACCGACTCCGAAGAATAGGCACCACATCCATTGTTCTAATGTGAGAGCTTTCGTGCTGAAC *F GCCATTTTACCATATTGTATGATAACTACCTGCGATAGACATGTTACGATCCAGATAGTGTAAAAGATGGGATTGGTGA *F ATATTCCTTGGAAGACATTACGCTGACCATGGATTTTTCTAGCGTTAAATTCGTTGAAAAGTGTCATCATGACGA *F This and vertebrate matches show that a longer C-terminus is needed for *F CG2165, and it is available in two exons in the genomic sequence. *F \**********A. mellifera Contig2806 *F AACACATGCAAAATGGAACCACTCTATAGGACAATCTGGATTATCACAACCTATCATTTCCCCATAAGAAACTTGATGA *F CATAGGCAATAAGTTGGTTCGTTAGGATCAACTGGCATATCTAAAACATCTGCTGGATGACCAAGTGCAGTAGAATCTA *F CTTGAGCACCACTTCCTACAGCTCCAGCTGATGAAGCAGAAGCAACAGATCCTCCTTTTTTCTGTTTTTTTCTAGCTGT *F TTTCGATTCATCTTCACTGTTAGTACCTGCACCTTTCTTTCGTTTTTCTTTTTCTTTTAATTTTTTCCTGCCCTTTTTA *F CTAGCATTATTTTCTTCTTGTGCCCTACTACTATTTAAAGCTTTATCTTGTATTTCAGCTTCAAATCTAGCCAAATCAG *F AATCTAGTCTCCTAATATGTTTATCAACTAATTCATATGTTTGTATTGCTAATTGTACTTTATCATCACCATATTCCTT *F TGCCTTGTTAAATAAGTTTTGAATATGAGTCAATTGTTCCTTTTTCTTTTCTGGGGATTCTTTCTTTACATTTTTTAAA *F TAATCATCTGCTAATTTATCTATATCTTTCATTAATCCTTGTGCTCTAGCATCAAGGTCTCGCATTAAAGTGAAATTTC *F TTTGTAATTCAATAGGTAGATGTTCCAAACTGTCTAAATAATGTTCTAAATACAGTGCTGTTGTCATGTTTATTGTTAC *F TTAAAGAAAAA *F This EST, and vertebrate matches, and even Drosophila cDNA LD46333, show that *F CG9293 has three open introns retained that need to be spliced out. *F \**********A. mellifera Contig321 *F TTGCTCCTTTTAGGATTGGATAATGCAGGAAAAACAACAATTTTAAAATCATTGGCCAGTGAAGATATTACACAGGTAA *F CACCGACACAAGGATTTAATATAAAAAGTGTTCAAAGCGAAGGTTTTAAATTGAATGTCTGGGACATAGGAGGTGCTCG *F AAAAATTCGACCTTATTGGCGAAATTATTTTGAAAATACAGATGTTTTAATATACGTCGTGGATAGTGCGGATGTAAAG *F AGATTAGAGGAAACGGGTCAAGAACTATCAGAACTTTTATTGGAGGAGAAATTGAAAGGTGTTCCATTATTAGTTTATG *F CGAATAAACAAGATCTTGGACAAGCAGTCACAGCAGCAGAAATTGCCGAAGGCCTCGGATTACATAATATCAAAGATCG *F CGATTGGCAGATACAATCGTGCATTGCTATCGACGGGAAAGGCGTGAAGGAGGGTCTTGAATGGGCATGCAAAAATATC *F AAAAGAAAGTAATATTGGCACAAGTGTCTTAAAAAATCGAACAGCTTACAATGTACTCTCATACATTCAACATACTTTT *F TATTTGGCTTTTATCTAATATTTACAAAAGCTGTTAACAGACAACGTAAACGAATGGATTTACTAGTCAATTAACTTCC *F GTCCTTCATGGAAGGAGCTTTTCTAAAGCTCTAATTTACGATGCCTTAATGAATACGATATCTATTATA *F This and vertebrate matches, and cDNA AT01916 indicate that there is an open *F intron that needs to be spliced from CG6560. *F \**********A. mellifera Contig457 *F ATATATACAGTTGCGACACAGTTGCCGGTGCGACACAACGCGATATTTAGTCGAGCGATATGCCGACGAGTTTGTTCAG *F CGAAATGGATCGCGGAGCTAATGGCGGTGGAACTGCTTCCCTCGAAGACCAGAGAGCCCTACAGTTGGCATTAGAATTA *F TCTATGCTTGGTCTCGAAGGTACTCCCGGATGTCCGGGCACTGGTACAGGAACCGGCACCGGAACGGCCAACGATCCGG *F ATCCTTTGCAAACGACACCGGCAGGCGTTTTCGAGGAAGCACGTTCTAAGAAGAGCCAGAATATGACCGAGTGCGTGCC *F GGTGCCTAGCAGCGAGCACGTGGCAGAGATCGTCGGCCGACAAGGTTGTAAGATCAAAGCGCTCCGAGCGAAGACCAAC *F ACCTACATCAAGACGCCGGTGCGCGGCGAGGAGCCGGTGTTCGTGGTGACCGGGCGCAAGGAGGACGTGGCCCGCGCGA *F AGCGCGAGATACTGTCGGCCGCCGAGCATTTCTCGCAGATCCGTGCCTCCCGCAAGAGCTCGTTGGGCGCCCTGTTGGG *F CGCGCCCCCCGGCCCGCCAGCCTCCGTTCCGGGCCACGTGACGATTCAAGTACGAGTCCCGTACAGAGTAGTCGGGCTT *F GTGGTAGGACCGAAGGGCGCGACCATCAAGAGAATCCAGCACCAGACCCACACCTACATAGTGACGCCGAGCCGCGACA *F AGGAGCCGGTGTTCGAGGTGACGGGGCTACCCGAAAGCGTGGAGGCTGCGAGGCGCGAGATCGAGGCACACATAGCGCT *F TAGAACCGGCACAGGAACCACCCTCGACGATTCGGAACTGCTAAGCGTGCTCTGTCGCGGTGGCCTGGGCTCGATCCTC *F GGTTGCCTCGACCCACCCGGCTCGAACGGCTCGAACGGATCCAGCGGAGCGTTCTCCAGCAGCGGCAGTTGCAGCAGCT *F CGTCCAGCAGTTCCGGCGCGCCCGGGCTCAACGATCTCGTCGCGATTTGGGGCGCCGGCATGGAGAGGGACGAGGGCCT *F GGGGGAGTCGCCCTCCTTCGAGTCCCAAACGGCGTCCGCCTCCTCGATCTGGTCGTTCCCAGGCGTCGCGCTACCCTCG *F AGGCCATCCCCGCCGGCGTCAGCGAGCCCGACGTCGCCCACGGACTCGCTGCTGGGCGGTGGACGGCGGGAATGCGTGG *F TGTGCGGCGAC *F This and vertebrate match indicate at least two exons missing from the *F N-terminus of CG11360; and they are encoded in Genome. *F \**********A. mellifera Contig49 *F TCAACCACCAACTCAAGTTACAACTTTAGATTGTGGTATGAGAATTGCTACTGAAGATAGTGGGGCACCAACAGCCACA *F GTTGGATTGTGGATTGATGCTGGTAGTCGTTTTGAAACTGATGAAAACAATGGAGTTGCCCATTTTATGGAGCATATGG *F CATTCAAAGGAACTACTAAACGTTCACAAACTGATTTAGAATTAGAAATTGAAAATATGGGTGCTCATTTAAATGCATA *F TACAAGTAGGGAACAAACAGTATTTTATGCTAAATGTTTGGCAGAAGATGTTCCAAAAGCTGTTGAAATTTTAAGTGAC *F ATCATTCAAAATTCAAAACTTGGCGAAAATGAAATAGAAAGAGAACGTGGTGGTATTTTAAGATAAATGCAGGAAGTTG *F AAACAAATCTTCAAGAAGTTGTTTTTGATCATTTACATGCTAGTGCTTATCAGGGTACACCATTAGGAAGAACAATTCT *F TG *F This and vertebrate matches and many ESTs show CG3731 needs N-terminus, and *F there is an exon in the genomic sequences *F \**********A. mellifera Contig663 *F AGGAGGTAATGTTTTTAAACGAACTCGAAGAAATTCTTGACGTGATCGAACCTGCGGAATTCCAAAAAGTTATGGATCC *F GTTGTTTAGACAATTAGCGAAATGTGTATCATCTCCGCACTTTCAGGTCGCTGAAAGAGCTTTGTATTACTGGAACAAC *F GAATACATAATGTCACTTATATCGGATAATTATTCAGTCATTCTACCAATTATGTATCCAGCATTCTATAGAAATTCCC *F GAAATCATTGGAACAAAACTATCCATGGTTTAATCTATAATGCATTAAAGCTTTTTATGGAGATGAATCAGAAAGTATT *F CGACGAGTGTACTCAACAGTATTATCAGGATCGACAAAGAGAAAGAAAACTTATGAAAGACAGAGATGAAGCGTGGATG *F CGCGTTGAAGCTCTGGCAATGCGACATCCAAATTACAACGCGACTATAAAAGGTATTACGAATACAACAATTGGCACAA *F TATCGCAACAACAATTGGACAGCCCTCCGCCCGATGAAGATGGTGATACTGATCAGACACCGCTTACATTGGAAAAAAT *F AGAGGCGAAAGCAAATGAGGCAAAAAAAATGACGAACTCTAACAAAACAAAGCCACTTTTACGGAGGAAAAGCGATTTA *F CCCCAAGACACGTATACAATGCGGGCATTATCTGATCACAAACGTG *F This and vertebrate matches show that CG7913 needs a different C-terminus, and *F it's available in the genomic sequences. *F \**********A. mellifera Contig75 *F CCAAATTCTACCCATGCAAGTAGTAATTCTTGTTTTGAATTTTGACGATTTTTTACAGTATAATAATGTTTCCTTAACC *F GCCATCCCATATCAGGAATGGCTTTACAAACTTCAAAATTTGCATCGCTACGTAATGCTAATGACACCTGTTGCAAAGC *F TATTTCATGTAATGGTGCTGTATAATTATCTGGATCTAAGAATTTTTTCATAGGAAGTGATGAAGCCAATATAGGATTC *F ATTAGTATATTGTTTAAATAATTCTGTAGGGCTATTTGACGTTGAGCTATGAAGTCTGGTTCCATATTACCAATGATTT *F TCTTTGGAGGAAATGCCAGATCAATGCCAGATATTGATAAAGCTGCATTAAGCTGTACAAAGTCATTGTAGCGTCTGCT *F AACTCTCCAAAATTTTTCAGAAAGTGGACCTCTTTGTGTTCTAATCACATATTCCGTATGTCCGTCGATTGTTCTTGCA *F TTTTCAATGACGCTCGTCAGCTTTTCTGTATCATCTAACAGCACTTTATTTGTATATCGTTTCTCAAATAAAGCCATTG *F ATCATTAGTCCAACAAAATGCAGAGCCCGTGCGGGGCGAACTGCGTATGGCTAGCGCATATTTTTCGTAAGTC *F This and vertebrate matches, and cDNA LD23236 show that CG8726 needs an *F N-terminal exon, and it's there in the genomic sequence. *F \**********A. mellifera Contig94 *F GGAATATTAAAGATTGTTTTCCAGATCTTCAAACGCAAGTTAAACTTAAATTACTTCTTTCATTTTTCCACATACCAAG *F ACGGAATGTCGAGGAGTGGCGTGTTGAATTGGAAGAAATCATTGAAGTGGCCTCATTGGATAGTGAATTATGGGTATCA *F ATGCTATCCGAAGCGATGAAAACATTTCCATCAACAGGTTCTTTGAATACAGACATTACAGATTTAGATGAACATAGGC *F CTATTTTTGGAGAACTAGTCAATGATCTTCGAAAACTTTTGAAAAAACAAAATGATCCAGCTATGCTTCCATTAGAATG *F TCATTATCTTAATAAGACTGCTCTTACTTCTGTGGTTGGCCAACAACCTGCACCAGTTAAGCATTTTACTTTAAAGAGA *F AAACCAAAAAGTGCTGCATTAAGAGCAGAGTTACTTCAAAAAAGTACAGATGCTGCAAGTAATTTAAAAAAAAGCACTG *F CTCCTACAGTACCTGTAAGAAGTAGAGGAATGCCTAGGAAAATGACTGACACAACACCTTTGAAAGGCATTCCCAGTAG *F AGTCCCTACAAGTGGTTTTCGTTCTCCCTCGCTTACGAGTTCTTCAATGTCTAACAGGACACCTC *F This and vertebrate matches, and cDNA SD13146 show that CG5874 needs at least *F two additional exons *F \**********A. mellifera Contig950 *F AACGAAGTTGGTAGTTTTGTAATGAGTTCTTTGCTTTCAAGGTAGAATTTAGGAAAGGATAAATTATTTTTTCGTAGTA *F TTCTTGAGAATTTATATTAAAAGATGGAGGACGTTCTCGAAGAGGTTGTTTCTTCTGATGATTTAAAGAAATTTGAACG *F TATATATAATGAGCAATTACGTTCATCAGTAATAACACAAAAAGCTCAATTTGAATATGCATGGTGTCTTGTTAGAAGC *F AAATATCCTGCAGATATCAGAAAAGGAATAATGTTATTGGAAGATTTATATTGCAATCATAGTGATAGCGAAAAACGGG *F ATTGTCTTTATTATTTAGCCATTGGAAATGCTAGAATAAAGGAATATACAAAAGCTTTAGCGTATGTCAGATCGTTTCT *F TCAGGTTGAACCTGGAAATCAACAAGTACAGCATCTGGAAACATTGATCAAGAAAAAAATGGAAAAAGAGGGACTTTAT *F GGTATGGCCATTGCAGGAGGAGTTATTATTGGTCTTGCAAGTATTCTCGGCCTCAGCATTGCTATGGTCAAAAGAAACT *F AATCATTTCATGTGAAAAAATCTGTAACTATTGTGTGATGTGAAATAGTTGCTTTGTACAACGCGTTATATATATTATT *F TATTGATTGAAATA *F This and vertebrate matches show that CG17510 needs an N-terminal exon, *F present in genomic sequences. *F \**********A. mellifera Contig982 *F GCAAGTGTCAATTTGATTATTGTCATCGTTTCTGTGACTATCGATAGTTCTGTAAGTTTGGAATTTCTACCGATGATCG *F ACAAATTGCATATACGTATTTCTGTGGGTGCCGTGAGGCGGCGAGAAAGGCCACCTCTTTAAATTAAAAGAAGGAATGA *F CGCTAGAGAATCCGTTCTTTGTCGTCAAGGACGAAGTTTGTAAAGCTTTAAATAAAAATCGCGGTTTATACGGGCGCTG *F GACGGAATTGCAGGATGTTGTCACGAGTCCTACTGTGAGTGGGGGAATCCCAATCTCACGCGACGAATTAGAGTGGACT *F ACTACTGAATTACGGAAAGCTTTACGTTCAATCGAATGGGATCTTGATGATTTAGAAGACACAATTTGTATTGTAGAAA *F AAAATCCAACAAAGTTTAAAATAGATAACAAAGAATTAACGGTTCAACGAAGTTTTATCGAACAAACTCGAGAAGAAGT *F TAAGACCATGAAAGATAAAATGAATTTAAGTAGAGGTCGGGATCGTGATAACACAGCAAGACAGCCACTTTTAGATAAT *F AGTCCTGCTCGAGTTCCTGTCAATCATGGCACAACAAAATATAGCAAATTGGAAAATGAAATTGATAGTCCAAATAGAC *F AATTTTTAGGAGATACCTTACAACAACAAAATGATATGATGAGACAACAAGATGAGCAACTAGATATGATAGGTGAAAG *F CATTGGAACATTGAAAACAGTATCTAGACAAATCAATACTGAATTAGATGAACAAGCAGTTATGTTAGATGAATTT *F This and vertebrate matches suggest that CG7736 needs different C-terminal *F splicing. *F \------------------------------------------------------------------------------ *F -- # *U FBrf0138601 *a Whitfield *b E. *t 2001.7.17 *T personal communication to FlyBase *u *F Date: Tue, 17 Jul 2001 14:09:40 \+0100 *F From: Eleanor Whitfield *F To: Gillian Millburn *F Subject: FlyBase error report \- 8 *F Hi Gillian, *F .. *F Acp32CD: *F Wolfner et al, Insect Biochem. Mol. Biol. 27:825-834(1997) *F Begun et al, Genetics 156:1879-1888(2000) *F Compared to translations from the above paper Celera sequence (AE003630; *F AAF53055) *F require numerous consecutive frameshift corrections to achieve the *F correct sequence. *F elav: *F .. *F Robinow et al, Science 242:1570-1572(1988), M21152; AAA28506 *F and EDGP, AL022139; CAB37430 *F Compared to above translations Celera sequence (AE003417; AAF45517) is *F missing the N- *F terminal exon that encodes the initiating Met. *F Mlc1: *F .. *F Falkenthal et al, Proc. Natl. Acad. Sci. U.S.A. 82:449-453(1985), *F M10125; AAA28711 *F Falkenthal et al, Mol. Cell. Biol. 4:956-965(1984), K01567; AAA28710 *F Compared to above translations Celera sequence (AE003761; AAF56733) is *F missing the N- *F terminal exon. *F Klp61F: *F .. *F Heck et al, J. Cell Biol. 123:665-679(1993), U01842; AAA03718 *F Compared to above translation Celera sequence (AE003471; AAF47458) has *F an extra *F intron that should not be there. *F nicastrin: *F .. *F Yu et al, Nature 407:48-54(2000), AF240470; AAG11414 *F Compared to above translation a bit of a mess is revealed! Celera *F sequence *F (AE003749; AAF56349) reveals a possible frameshift in the original *F sequence *F (YATLYPRKPAIENN \-> SPPCTPESQQSETT). In addition Celera is missing the *F N-terminal exon, *F have an incorrect splice site and the wrong C-terminal exon. *F Bgb: *F .. *F Golling et al, Mol. Cell. Biol. 16:932-942(1996), U22177; AAB03676 *F Compared to above translation Celera sequence (AE003472; AAF47533) shows *F an upstream *F initiating Met. *F FK506-bp1: *F .. *F Theopold et al, Gene 156:247-251(1995), Z46894; CAA86996 *F Compared to above translations Celera sequence (AE003708; AAF55172) is *F missing the N- *F terminal exon. *F Ef2b: *F .. *F Grinblat et al, Nucleic Acids Res. 17:7303-7314(1989), X15805; CAA33804 *F Compared to above translations Celera sequence (AE003781; AAF57226) is *F missing the N- *F terminal exon. *F thanks *F Ele # *U FBrf0138602 *a Wilson *b I. *t 2001.9.18 *T personal communication to FlyBase *u FlyBase error report for CG12366 on Tue Sep 18 07:12:00 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 18 Sep 2001 07:12:00 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: iwilson@edv2.boku.ac.at *F Subject: FlyBase error report for CG12366 on Tue Sep 18 07:12:00 2001 *F Error report from Iain Wilson (iwilson@edv2.boku.ac.at) *F Gene or accession: CG12366 *F Release: 1 *F Missed gene *F Comments: The protein encoded by this gene has homology to the human protein *F O-fucosyltransferase (POFUT1); see Wang et al. J. Biol. Chem. (2001) *F 10.1074/jbc.M107849200. The human enzyme catalyses, for example, the *F O-fucosylation of Notch in order to generate the substrate for fringe. # *U FBrf0138603 *a Naveira *b H. *t 2001.9.21 *T personal communication to FlyBase *u *F From: 'Horacio Naveira' *F To: 'Gillian Millburn (Genetics)' *F Subject: RE: New natural transposable element of D. melanogaster *F Date: Fri, 21 Sep 2001 12:49:15 \+0200 *F Dear Gillian, *F I am sending you as an attached file the information you requested about *F Pilgrim. *F Subsequent to the submission of our manuscript for publication (two months *F later, to be more precise), another manuscript was submitted by others *F (Bowen and McDonald, Genome Research 11, 1527-1540, 2001), which reported a *F similar phylogenetic characterization for several members of the mdg1 *F lineage ot the Ty3/gypsy group. In their report, the element wolfman is *F identical to Pilgrim, and therefore both names should be considered *F synonyms. *F Yours sincerely, *F Horacio *F Dr. Horacio F. Naveira *F Dep. Biol. Cel. y Molec. *F Univ. A Coruña *F campus da Zapateira s/n *F 15071 A Coruña *F Spain *F .. *F \------------------------------------------------------------------------------ *F -- *F DEFINITION Drosophila melanogaster Pilgrim retrotransposon inserted *F at genomic clone AC007146 (nucleotides *F 115349-122693) *F LTR 1..506 *F /standard_name='5' LTR' *F CDS 1229..1456 *F /gene='sORF' *F /note='homologous to sORFs from 412, mdg1, blood and *F Stalker' *F /translation='MGWFGSDDSQTKDNTANVVNNVKIVDHTEDIQALWVLLLILTIT *F SVAQFLLTLYIKHNKILKRRYMSRANNLDRV' *F CDS 1531..2851 *F /gene='ORF1' *F /note='gag homologous gene' *F /translation='MRTGNYIVPHNFHVVDDNFPIPGDGIVGIDFIKTFNCQLDFTTE *F SDFFILRPNNIKQAIQIPIFHNIDNTEITIPSRCEVIRQITVSSVDNQILIPNQEIED *F GVFVGNSISDSKNTYIRILNTTNSNKILNVNKINFEPLTSYKIADLNDSIRAESILSR *F LKKNFPSAHKKMLTELCSQYTDIFGLETEPITTNKFYKQKIRLRDDEPSYIKNYRTPH *F SQQAEISRQVTKLIEDKIVEPAVSEYNSPLLLVPKKSLPDSKEKKWRLVIDYRQINKK *F LLADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELEEDSRNITSFSTSSGSYRFT *F RLPYGLKIAPNSFQRMMTMAFTGLEPSQAFLYMDDLIVIGCSEKHMTKNLTNVFELCR *F KNNLKLHPDKCSFFMSEVTFLGHKCTDKGILPDDTKYDAIQRYPVPTDADSARRFVAF *F CNYYRRFIQNFADYSRHITRLCKKNVKFEWTADCQHAFEHLKKQLMNPTLLKYPDFSK *F EFCIITDASKEACGAVLTQNYNGIHLPVAYASRSFTKGESNKSTTEQELSAIHWAINH *F FKPYIYGRHFTVKTDHRPLTYLFSMVNPSSKLTRMRLDLEEYEFTVEYLKGKDNYVAD *F ALSRITIDQLKNISKQVLKVTTRNQSRQESCAGKGNKNDKVELPKQTTQEASKPKVYG *F VINNDEVRKVVTLHVNNMICFFKHEKKITARYNVEDLYINGTLDLGQFFHRLEKQAGM *F HNINQLKMAPWENIFDNISIDTFKKMGNKTLKLLRVALLNPVTLVNTKKEKEAILSTH *F HDDPTQGGHAGITKTLARIKRHYFWKGMTREITEYIRKCPKCQKAKIMKHTKTPLSIT *F ETPISAFDRVIVDTIGPLPKSENGNEYAVTLICDLTKYLVTIPIANKSANTVAKAIFE *F SFVLKFGPMKTFISDMGTEYRNSIIKDLCKYLKVENITSSAHHHQTVGTVERSHRTFN *F EYIRSYISVDKTDWDVWIKYFEFCFNTTPSMAHNYCPYELIFGKTCNLPKHFNSTDRI *F EPIYNIEDYPKESKYRLEVAYNRARIMLEEQKKKNKELYDLKLNDISISIGDKVLLKN *F ETGHKLDFKYTGPYTVVKIEERDNIVISNDKKKPQTVHKDRLKLFSS' *F CDS 2787..6479 *F /gene='ORF2' *F /note='pol homologous gene' *F /codon_start=1 *F /translation='MRTGNYIVPHNFHVVDDNFPIPGDGIVGIDFIKTFNCQLDFTTE *F SDFFILRPNNIKQAIQIPIFHNIDNTEITIPSRCEVIRQITVSSVDNQILIPNQEIED *F GVFVGNSISDSKNTYIRILNTTNSNKILNVNKINFEPLTSYKIADLNDSIRAESILSR *F LKKNFPSAHKKMLTELCSQYTDIFGLETEPITTNKFYKQKIRLRDDEPSYIKNYRTPH *F SQQAEISRQVTKLIEDKIVEPAVSEYNSPLLLVPKKSLPDSKEKKWRLVIDYRQINKK *F LLADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELEEDSRNITSFSTSSGSYRFT *F RLPYGLKIAPNSFQRMMTMAFTGLEPSQAFLYMDDLIVIGCSEKHMTKNLTNVFELCR *F KNNLKLHPDKCSFFMSEVTFLGHKCTDKGILPDDTKYDAIQRYPVPTDADSARRFVAF *F CNYYRRFIQNFADYSRHITRLCKKNVKFEWTADCQHAFEHLKKQLMNPTLLKYPDFSK *F EFCIITDASKEACGAVLTQNYNGIHLPVAYASRSFTKGESNKSTTEQELSAIHWAINH *F FKPYIYGRHFTVKTDHRPLTYLFSMVNPSSKLTRMRLDLEEYEFTVEYLKGKDNYVAD *F ALSRITIDQLKNISKQVLKVTTRNQSRQESCAGKGNKNDKVELPKQTTQEASKPKVYG *F VINNDEVRKVVTLHVNNMICFFKHEKKITARYNVEDLYINGTLDLGQFFHRLEKQAGM *F HNINQLKMAPWENIFDNISIDTFKKMGNKTLKLLRVALLNPVTLVNTKKEKEAILSTH *F HDDPTQGGHAGITKTLARIKRHYFWKGMTREITEYIRKCPKCQKAKIMKHTKTPLSIT *F ETPISAFDRVIVDTIGPLPKSENGNEYAVTLICDLTKYLVTIPIANKSANTVAKAIFE *F SFVLKFGPMKTFISDMGTEYRNSIIKDLCKYLKVENITSSAHHHQTVGTVERSHRTFN *F EYIRSYISVDKTDWDVWIKYFEFCFNTTPSMAHNYCPYELIFGKTCNLPKHFNSTDRI *F EPIYNIEDYPKESKYRLEVAYNRARIMLEEQKKKNKELYDLKLNDISISIGDKVLLKN *F ETGHKLDFKYTGPYTVVKIEERDNIVISNDKKKPQTVHKDRLKLFSS' *F LTR 6840..7345 *F /standard_name='3' LTR' *F BASE COUNT 2933 a 1304 c 1288 g 1830 t *F ORIGIN *F 1 tgtaatgtgc acacatatcg aataagcact gtatcaaatc agaacattgg gaacaatcac *F 61 attgtagcac ttttgcaaca atgtttatgt aagacttttc agttcccagg catatcttga *F 121 gtgctcagca atctgaccac acaagaatgc tattcagacc agaagtgcag agtcagcata *F 181 attagcatgc gcgactgctc gatctttatg tccacatgac tctcttagac agcggcgctc *F 241 tcgctgccaa agttaagtac ataagagcaa agcaacgtct ctgccgacgg cctctctgcc *F 301 ggcgcagacg gattgcattt ggctagcttg gactcttcta gaccaagtac aaggcagtcg *F 361 taaaggagtc gtcaaagagc cttcaacatg tcctaattga atattaatga gtcttaacag *F 421 aagttacaat tttactgata tcatactgaa tctctatttc aataaaagta atataaagaa *F 481 cacaaaaatg cattacaatt attacatggc gaccgtgaca tggtcactta agcctgtaaa *F 541 acaataataa aaagaaatat ataaaagtta aaatgcgaac agtgaccatt aaaaaataaa *F 601 attgtgacaa ttgatcaaac ccagacgaca acagaagccg caaactggag gactctgctc *F 661 ctatcgagca aagggacgca cctactctac aagaggcact agaggtgtgt ccaaacgatg *F 721 gaccacgccc actcacaata gctgagtata gggcaagaag acagccgaaa ccaaaaataa *F 781 agaagaaaag aagcggcaaa aggatcaagc ttcttcaaca acggagatta ataaaggacc *F 841 taataaagac ggcaactacg gaggaagaga aaactaacca agccaaaaat ctggaagcga *F 901 ttgaagccaa attgtgcagt ggtgcgcaat aacgcagttg aggctgtatt taataccaat *F 961 gccccaatct gccttaatat taaaaataca tacctcaagc cgatgcgtga aaacgctgct *F 1021 tgaaaaatac taatctctgt taggctttta ctaaaaacat gtgtacggta ttgaattata *F 1081 tagagcaaaa cattgtaaac cgtacacatg ccctctaact aactttttct tggacagaca *F 1141 tacgtgtagg gagaaattat agcaatatac aatttttaat caaaatttaa taaataaata *F 1201 aataataata atcgttcaat taacaactat gggttggttc ggatctgacg atagtcagac *F 1261 caaagacaac acggctaatg tcgtgaacaa cgtcaaaatt gtagatcaca cagaagatat *F 1321 tcaagcactg tgggttttac tcctaatttt gacaattaca agtgtggccc agttccttct *F 1381 gacgttgtac ataaaacaca acaaaatatt aaaaagaaga tatatgagta gggcgaataa *F 1441 tctagacagg gtttaaaaga aaaaaaaaaa atatatttta aagaatgagt atataaatat *F 1501 atactaacaa gtaggaatat ataaaatagt atggaatgga acgagttatg taaaaacata *F 1561 acaagaataa gaaacgaatt tgaaaaatct cataaatgtt tgtcgcaaaa tagacctatt *F 1621 ttaggaccga caacaaagaa acatgcaaat attctggtaa attccttcaa cgaagcacga *F 1681 atactagtgt atgataacaa ggaaagacta aatccagatc attggtctca ggtatcgaaa *F 1741 gttctcataa aacttagatc gaacttgtta tctgtaaaat tgaaacttgg tttggatata *F 1801 tcaataccaa ccattttaaa ttcgccaata aaaatagaat cggacgaaca aacagaaaca *F 1861 gaaatagaag acgaagattt aaacaactta acaattccag ctatattaac actagctgaa *F 1921 ttgacggagg aagaattggc tgagtcagac atagaagaaa cagaaacaaa atctgtcatc *F 1981 atggtggacg aagcagctgc ccagagggca tacataaaag acatttcaac ggcgattccg *F 2041 gaatttgacg gtaaaaagat caatttgcgt agatttatca cggcaatcaa gttggttaac *F 2101 ctgactaagg gaccacatga agctattgcc attgaagtga ttaaatcaaa aataatcgga *F 2161 acaacactct atagagtaca gaatgaagtt acaattgatg ccataatcag gaaattagaa *F 2221 gaagtggtcg taggagaaac aacggacgta gtaagagcaa aaatggcaaa tgtttaccaa *F 2281 aaaggtaaaa cagccacaca atttacaaat gaaattgaaa acctacgcaa gtctcttgaa *F 2341 tcttcatata tagatgaagg attgcaacca gaacatgcta tcaaatttag cacaaaggaa *F 2401 gcaatcaata caatgacaaa gaattgcgat catggaaaac tgaaagcaat cctagaggca *F 2461 ggaacattta agacaatgga cgaagcaata agtaaataca tccattgcag cactgagatg *F 2521 acaggaagtg caagttccgt tttgttttac aagagaggac aaggaagtta caccagaggt *F 2581 aactaccgag gacgaggaaa tggtcgaggt ggtaataata gaaataatta taaccagaac *F 2641 accggccaat acaacaactt taataattat aacaatagca atggcagagg acgaggagga *F 2701 tatagagaat ataactacca gaacagaggc ggtggtaact ataacaacca aaaccgcaat *F 2761 ttctcaagtt atagccaaaa tggtaatgtc agacatgcac aaggcacgtc ggaaaaccag *F 2821 caggctccct tagggcatca agaacagtaa aacaaaaggt tcataccatc aatcttaatc *F 2881 taaatatttt cattaaagta aaaaatgaac acacaaataa gatacttaca tttctagtag *F 2941 acacaggcgc cgatatatca gtcattaaag aaaactcaga tgaacttttg aatcttgatc *F 3001 ataataatat aacacaaatt acaggaattg gaaagggttc aataaattca ataggtttaa *F 3061 cacttctcga gatgagaacc ggtaactata tagtaccgca caattttcat gttgtggacg *F 3121 acaattttcc gatccctggt gacggaatag ttggaatcga cttcataaaa acattcaatt *F 3181 gccaattaga ttttactaca gaaagtgatt ttttcattct aagaccaaac aatattaaac *F 3241 aagcgataca aataccaatt tttcataata tcgataacac tgagataacc ataccatctc *F 3301 gttgtgaggt tattaggcaa attaccgtaa gttcggtgga taaccaaatt ctaataccca *F 3361 atcaggaaat agaagatggt gtatttgtcg gaaattcgat atcagattca aaaaacacgt *F 3421 acatccgaat actaaacaca actaacagca ataaaatttt aaatgtaaac aaaatcaatt *F 3481 ttgaaccatt aactagctac aaaatagcag acctaaacga ctccataaga gccgaatcaa *F 3541 ttttaagcag attaaagaaa aactttccat ctgcacataa gaaaatgttg actgaactgt *F 3601 gttcacaata tactgatatt tttggattgg agacagagcc aatcactaca aataaatttt *F 3661 acaaacaaaa gataagattg agagatgatg aaccaagcta tattaagaac tatagaacac *F 3721 cccattcaca acaagccgaa atatcgagac aggtaactaa attaatagaa gacaagatag *F 3781 tagaaccagc tgtatctgaa tacaatagcc cattattgct agttccaaag aaatccttac *F 3841 cggattcaaa agaaaaaaaa tggcgtttag tgatagacta tcgccaaata aataaaaagt *F 3901 tgttagctga caagttcccc ttgccaagaa tagatgacat acttgatcaa ttaggccgag *F 3961 caaaatattt ctcgtgcctg gatcttatgt cgggattcca tcaaatagag ttagaagaag *F 4021 attctcgaaa tataacatcc ttttctacga gcagtggctc ttatcgcttc acgcgactac *F 4081 catacggatt aaaaatagct ccaaattcct ttcaacgaat gatgacaatg gcatttacag *F 4141 gtctggaacc atctcaagca tttctgtaca tggatgactt aattgtcata ggctgttctg *F 4201 aaaaacacat gaccaagaat cttacaaatg tttttgaact atgcaggaaa aacaacctca *F 4261 aactgcatcc agataagtgc tcatttttca tgagtgaagt cacttttctt ggacacaaat *F 4321 gtactgataa aggcatattg cctgacgata ctaaatatga tgctatacag agatatccag *F 4381 ttcctactga cgcagacagt gccagaagat tcgtagcatt ttgtaactat tatagacgtt *F 4441 ttatacaaaa ttttgcagat tattcccgtc acataacaag attatgtaag aaaaatgtaa *F 4501 agttcgaatg gacagctgat tgccagcatg ccttcgaaca cctgaaaaaa caactaatga *F 4561 atccaacttt gctgaaatac cccgacttta gcaaagagtt ctgtattata actgatgcaa *F 4621 gcaaagaggc atgcggagcg gtactgaccc agaactataa tggtatccat ttgccagtag *F 4681 cttatgcatc ccgtagcttc actaagggtg agagcaacaa gtcaacaaca gagcaagagt *F 4741 tgtcagcaat acattgggcg ataaatcatt tcaaaccata tatatatggt agacacttca *F 4801 ccgtcaaaac agatcacaga ccgttaacat acttgttctc gatggttaat ccaagctcaa *F 4861 aactgaccag aatgaggctt gatttagaag aatatgaatt tactgtggaa tatcttaagg *F 4921 gaaaagacaa ttacgtagca gatgcgttat ccagaataac catcgaccaa ctaaaaaata *F 4981 tatccaaaca agtacttaaa gtcactacaa gaaatcaaag tagacaggaa tcctgcgcag *F 5041 gaaaaggaaa taaaaatgat aaagtagaat tgcctaagca aactactcaa gaagcttcta *F 5101 agcccaaagt atacggagtc attaataatg acgaagtacg caaagtagtg acattgcatg *F 5161 taaataatat gatatgtttt tttaaacatg aaaaaaaaat tactgcaaga tacaacgttg *F 5221 aagatttgta tattaatgga actctcgact taggtcaatt tttccacagg cttgaaaagc *F 5281 aggccggtat gcataatatc aatcaactta aaatggcacc gtgggaaaat atctttgata *F 5341 acatttcaat agatacattt aagaaaatgg gcaataaaac attaaaacta ttaagagtag *F 5401 cgctactcaa cccggtgacc ttagtgaata caaagaaaga aaaagaagca atcctgtcta *F 5461 cacaccacga cgatccaaca caaggaggac acgcaggcat tacaaaaacc ctggccagga *F 5521 ttaaaagaca ttacttttgg aaaggtatga ctcgggaaat aacagagtac atacggaaat *F 5581 gtccaaaatg ccaaaaagct aaaattatga aacacacaaa aactccttta tcaattacag *F 5641 agacaccaat aagcgcattt gacagagtca tagtggatac gataggtcca ctaccaaagt *F 5701 cagaaaacgg taatgaatat gctgttacac ttatatgcga cctgacaaaa tatttggtaa *F 5761 ccattccaat cgcaaataaa agcgcaaata cagtagcgaa agcaatattt gaatcctttg *F 5821 tactaaagtt cggtccaatg aagacgttca tttcggacat gggaactgaa tacagaaatt *F 5881 caattatcaa agacttatgc aaatacctga aagtagaaaa tataacttct tcagcacacc *F 5941 atcatcagac ggtaggaaca gtggaaagaa gtcatagaac ttttaacgaa tacatccggt *F 6001 catacatatc ggttgataaa actgactggg acgtatggat aaaatatttc gaattttgtt *F 6061 ttaatactac accatctatg gcacataatt attgtccata tgagttgatt ttcggtaaaa *F 6121 catgcaattt accaaagcat tttaatagca cggatagaat agaacccatt tataatatag *F 6181 aagactatcc taaagaaagt aaatataggt tagaagtagc atataacaga gcaagaatta *F 6241 tgctcgaaga acagaagaaa aagaataaag aattatatga cttaaaatta aacgatataa *F 6301 gtatatcaat aggagataag gtgttattaa aaaacgaaac cggacataaa ttagatttca *F 6361 aatatactgg accatatacg gtagtaaaga ttgaagaaag ggataatata gtaatatcaa *F 6421 atgataagaa aaaaccacaa acggtacata aggataggtt aaaattgttt agttcttaaa *F 6481 aaaaaaaaat aaataatatg gtggccacca gcaaaaaaaa aaaatatata tagagaaaag *F 6541 agttaacccc acataagtgc attgaatgta agaaaacatt tttcttttat catctttgat *F 6601 ggttgaacga ttataaacta aaaatttgaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa *F 6661 accaaaaaaa aacccccaag aacacgtatg tttgtacaaa atgttcctta aatttcctta *F 6721 acattaaaat tacttcctta aaaaaaaaaa aaaaaaaaaa aaaaaaaaat atatatatat *F 6781 aaaattttta attataaaat aacttcataa aattacgtta ttttccaaaa ggagggagat *F 6841 gtaatgtgca cacatatcga ataagcactg tatcaaatca gaacattggg aacaatcaca *F 6901 ttgtagcact tttgcaacaa tgtttatgta agacttttca gttcccaggc atatcttgag *F 6961 tgctcagcaa tctgaccaca caagaatgct attcagacca gaagtgcaga gtcagcataa *F 7021 ttagcatgcg cgactgctcg atctttatgt ccacatgact ctcttagaca gcggcgctct *F 7081 cgctgccaaa gttaagtaca taagagcaaa gcaacgtctc tgccgacggc ctctctgccg *F 7141 gcgcagacgg attgcatttg gctagcttgg actcttctag accaagtaca aggcagtcgt *F 7201 aaaggagtcg tcaaagagcc ttcaacatgt cctaattgaa tattaatgag tcttaacaga *F 7261 agttacaatt ttactgatat catactgaat ctctatttca ataaaagtaa tataaagaac *F 7321 acaaaaatgc attacaatta ttaca *F // *F \------------------------------------------------------------------------------ *F -- # *U FBrf0138604 *a Levis *b R. *t 2001.7.6 *T personal communication to FlyBase *u *F Date: Fri, 6 Jul 2001 17:06:03 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: MdaPK gene may = gek gene (?) *F The FlyBase report for the gene MdaPk describes it as having a *F putative molecular function as a protein serine/threonine kinase, as *F inferred from sequence similarity. Its location at 60B10-11 is based *F on the in situ hybridization position of the P element insertion in *F l(2)09373, which is the only known mutant allele. The two flanking *F sequences, which were recovered by inverse PCR with primers for the *F two ends of l(2)09373, map to different sites on the genome sequence, *F approximately 16 kb apart. The 3' flank maps very near the annotated *F 5' end of the gek gene. The FlyBase report for the gek gene also *F lists it as having a putative molecular function as a protein *F serine/threonine kinase, as inferred from sequence similarity. Its *F estimated cytogenetic location is 60B4-5. The 5' flank of l(2)09373 *F maps to a site between the 5' ends of the divergently transcribed *F genes CG4049 and CG3253. Neither of these genes has sequence *F similarity to a protein kinase. Looking over this information, it *F strikes me that MdaPk is probably the same gene as gek. The separate *F map positions of the two flanks of l(2)09373 suggest the possibility *F of two insertions 16 kb apart or a 16 kb deletion. Recessive lethal *F alleles of gek have been described. However, one cannot be certain *F without further testing whether the lethality of l(2)09373 is *F associated with the insertion/breakpoint adjacent to gek or between *F CG4049 and CG3253. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0138605 *a Luo *b L. *t 2001.9.24 *T personal communication to FlyBase *u *F Date: Mon, 24 Sep 2001 22:30:44 \-0700 *F To: Gillian Millburn (Genetics) *F From: Liqun Luo *F Subject: Re: FlyBase query: gek *F Dear Gillian, *F Indeed the P-element we used was 9373. I am pretty sure that the *F original P-element has a deletion (rather than two P-element *F insertion) based on sequencing of the genomic DNA clones 5' to the *F P-element. This deletion removes at least the enok gene (please see *F our recent publication by Scott et al., Current Biology 11: 99. In *F the supplemental material there is also some discussion of the *F phenotypes attributed to enok and gek, respectively). *F Best wishes, *F Liqun Luo *F >Dear Dr. Luo, *F > *F >I am a curator working for FlyBase and I have a question about your *F >paper: *F > *F >Luo et al., 1997, Proc. Natl. Acad. Sci. USA 94(24): 12963--12968 *F > *F >which is about the gek (genghis khan) gene. *F > *F >In this paper you describe a P-element allele of gek. In the Materials *F >and Methods under the 'Molecular Biology' section you say that this *F >P-element was 'obtained from the Drosophila Genome Project'. From *F >looking at the Genome Project P-elements in this region, I think that *F >this P-element must be the 'l(2)09373' line \- as this maps to gek (see *F >below)- I would be grateful if you could confirm whether this is the *F >case (if I am wrong could you tell me which P-element line it does *F >correspond to). *F > *F >The sequence flanking the 3' end of the P-element in the 'l(2)09373' *F >line matches the 5' end of gek, while the sequence flanking the 5' end *F >of the P-element maps about 16kb upstream of gek. This either means *F >that there has been a deletion associated with the insertion of the *F >P-element in this line or there are two P-elements inserted 16kb apart *F >in the genome. *F > *F >In the Materials and Methods you say 'The sequence from the rescued *F >plasmid did not match the sequence from nucleotides 1-51 of the gek *F >cDNA, or the sequence of the corresponding genomic DNA, and yet the *F >clone maps to 60B by chromosome in situ hybridization, indicating that *F >a deletion is associated with the P-element insertion'. *F > *F >(Assuming that the gek allele in your paper is l(2)09373), have you *F >sequenced this region of the genome in the gek mutant line \- do you *F >know that there is definitely a deletion rather than two P-elements *F >inserted close together ? *F > *F >Any information you give me about this line will be curated as a *F >personal communication to FlyBase, *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F \-- *F =================================================================== *F Liqun Luo, Ph. D. Tel: (650)723-6645 *F Assistant Professor Fax: (650)723-0589 *F Department of Biological Sciences Dept Fax: (650)723-6132 *F Herrin Labs 144A E-mail: lluo@stanford.edu *F 385 Serra Mall *F Stanford University *F Stanford, CA 94305-5020 *F WWW: http://www.stanford.edu/group/luolab *F =================================================================== # *U FBrf0138606 *a Bayraktaroglu *b L. *t 2001.8.27 *T personal communication to FlyBase *u *F From leyla@morgan.harvard.edu Mon Aug 27 19:13:08 2001 *F Subject: P{EP}EP3312 is an allele of RhoL *F To: cambridge-curators@morgan.harvard.edu *F Dear Cambridge Curators, *F P{EP}EP3312 is an allele of RhoL, based on alignment (while making *F a 'SEAN/ARGS' for RhoL). *F >From EP(3)3312 accession AQ073904: *F 'The P element insertion position is base 210 in the 294 bases. This *F insertion position refers to the first base of the 8 base target *F recognition sequence.' *F Base 210 of AQ073904 corresponds to the 5th nucleotide of the *F published RhoL mRNA in accession AB035355. *F Aubrey, could you place accession AQ073762 associated with the *F P{EP}RhoLEP888 allele, *F and if CamCur make an allele of P{EP}EP3312, its associated *F accession AQ073904, under DNA/RNA accessions for RhoL? *F Thanks very much. *F Leyla # *U FBrf0138607 *a Hassan *b B. *t 2001.8.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Aug 23 14:40:40 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Lsp2-GAL4.H} construct and insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Bassem Hassan, Baylor College of Medicine (8/01). *F In the P{Lsp2-GAL4.H} construct, Lsp2 regulatory sequences drive expression *F of GAL4. The transformation vector was P{CaSpeR-4} into which a full *F length GAL4 cDNA was cloned at the NotI site. The construct was *F transformed into a y1 w1118 strain. A homozygous viable and fertile *F third chromosome insertion called P{Lsp2-GAL4.H}3 was recovered. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0138608 *a Umea Stock Center *b ?. *t 2001.6 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Aug 23 15:22:00 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Dp(1;4)mxc+R *F The following information accompanied a stock received from the Umea Stock *F Center (6/01). *F Dp(1;4)mxc+R has breakpoints 8D3-8;8D9;101A-102F and duplicates the mxc *F locus. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0138609 *a Wakabayashi-Ito *b N. *t 2001.8.27 *T personal communication to FlyBase *u *F From nito@helix.mgh.harvard.edu Mon Aug 27 18:37:53 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Enhancer of cactus E10 *F Dear Flybase, *F I would like to update my data. *F In Drosophila conference (1996) I did a poster presentation (Ito, *F N., Belvin, M., Anderson, K. (1996 ). Identification and *F characterization of new genes required for dorsal-ventral axis *F formation using the cactusE10 allele. A. Dros. Res. Conf. 37 1996 *F :187), in which I mentioned three putative genes, Enhancer of cactus *F E10 , number 7 , 11, and 81: abbreviated as E(cact E10 )7 , 11, 81. *F E(cact E10 )7 has been more characterized and renamed 'fusilli'. The *F reference is Wakabayashi-Ito N, Belvin MP, Bluestein DA, Anderson KV. *F ( 2001 ). fusilli, an essential gene with a maternal role in *F Drosophila embryonic dorsal-ventral patterning. Dev Biol 229:44-54. *F Thank you very much. *F Best wishes, *F Noriko *F \------------------------------- *F Noriko Wakabayashi-Ito, Ph.D *F Massachusetts General Hospital *F Cancer Center, Rm.7308 *F 13th Street, Building 149, *F Charlestown, MA 02129-2060 *F Phone : 617-724-2282 *F FAX : 617-726-6857 *F e-mail : nito@helix.mgh.harvard.edu # *U FBrf0138610 *a Bloomington Drosophila Stock Center *b ?. *t 2001.8.28 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Mon Sep 17 20:54:21 2001 *F Subject: Balancer Variants *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Balancer Variants *F Dated: 28 August 2001 *F Background: The following balancer variants are in stocks in the *F Bloomington collection but are apparently not in FlyBase. *F Binsc, oc1 ptg1 *F ClB, B36d *F CxD, ryBW *F CyO, Df(2L)noc20 *F CyO, Df(2R)cn-S3 *F CyO, Df(2R)cn-S6 *F CyO, Dp(2;2)M(2)m+ *F CyO, P{w+mC=Crew}DH1 *F CyO, P{w+mC=GAL4-Hsp70.PB}TR1, P{w+mC=UAS-GFP.Y}TR1 *F CyO, P{y+mDint2 wBR.E.BR=SUPor-P}RR1 *F CyO, Roi1 bw1 *F CyO, Roi1 cn2P bw1 *F CyO, Roi1 cn2P bw1

*F CyO, Roi1 sp*

*F CyO, S* bw1 *F CyO, bw* *F CyO, dpov1 pr1 cn1 *F CyO, necJR *F CyO, pr1 cn2 bw1 *F DcxF, Sb1 sr1 es *F FM1, B+ l(1)** *F FM1, lz+ *F FM3, vOf *F FM4, w1 B+ *F FM4, w1 f1 B+ *F FM6, w* ct* *F FM6, w1 *F FM6, wi dm+ *F FM6, y31d w* B1 *F FM7a, B+ *F FM7a, Df(1)FM7, l(1)** *F FM7a, In(1)wm73 *F FM7c, B+ *F FM7c, sn+ *F FM7c, w+ *F Insc, fu1 *F SM1, Got21 *F SM1, Hex-C4 *F SM1, L4 *F SM1, Pgk4 Pgi4 *F SM1, Roi1 *F SM1, dplvI pr1 Bl1 L4 *F SM6a, bwk1 *F TM1, Su(ss)2 *F TM1, T(2;3)D7, red1 D7 *F TM1, jv* *F TM1, mwh1 rhove-1 *F TM1, pp *F TM1, red* *F TM1, st* *F TM2, pp *F TM3, ryRK Sb1 Ser1 *F TM3, AntpDC Sb1 *F TM3, Au1 ryRK Sb1 Ser1 *F TM3, IdhS SodS Pgm4tr Sb1 Ald4 Ser1 *F TM3, In(3LR)D6, D6 ryRK Sb1 Ser1 *F TM3, P{UAS-y.C}MC2, Ser1 *F TM3, P{ry+t7.2=ftz-lacZ.ry+}TM3, Sb1 ry* *F TM3, P{ry+t7.2=ftz/lacC}SC1, ryRK Sb1 Ser1 *F TM3, P{ry+t7.2=sevRas1.V12}FK2, Sb1 *F TM3, P{w+mC=35UZ}2, Sb1 *F TM3, P{w+mC=GAL4-Hsp70.PB}TR2, P{w+mC=UAS-GFP.Y}TR2, y+ Ser1 *F TM3, P{w+mC=UAS-Dl.DN}TJ1, Sb1 *F TM3, Sb1 ry* *F TM3, Sb1 tld17 *F TM3, kls* ru* Sb1 *F TM3, p+ Sb1 *F TM3, red* Ser1 *F TM3, ry* su(Hw)2 Sb1 *F TM3, ryRK Sb1 Ser1 *F TM3, ryRK red2 Sb1 Ser1 *F TM6, Est-6F Est-CS FdhF Lap-DS Acph-1B Tpi4 *F TM6, Est-CS *F TM6, IdhS *F TM6, Ubx+ *F TM6, UbxP15 e1 *F TM6, h1 *F TM6, su(Hw)5 *F TM6B, Bri1, Tb1 *F TM6B, Dl* *F TM6B, P{w+mC=35UZ}DB1, Tb1 *F TM6B, P{w+mC=Crew}DH2, Tb1 *F TM6B, P{w+mW.hs=Ubi-GFP.S65T}PAD2, Tb1 *F TM6B, Tb+ *F TM6B, Tb+ ca1 *F TM6B, Tb+ e+ *F TM6B, Tb1 DrMio *F TM6B, Tb1 ca1 *F TM6B, glBS1 Tb1 *F TM6B, red1 Tb1 *F TM6B, ryCB Tb+ *F TM6B, ryCB Tb1 *F TM6B, ryCB Tb1 ca1 *F TM6C, AntpHu Sb1 Tb1 *F TM6C, Sb1 *F TM6C, Sb1 Tb1 *F TM6C, ca1 *F TM6C, cu1 Sb1 ca1 # *U FBrf0138611 *a Clark *b D. *t 2001.8.21 *T personal communication to FlyBase *u *F From clarkd@unb.ca Tue Aug 21 01:02:37 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Prat gene names *F Dear Flybase, *F I am writing a paper involving an analysis of sequence and expression *F patterns for three genes related to Prat at 84E. *F One is a D. melanogaster gene called CG10078 in 65D. It has 77% amino acid *F identity to Prat. It has has many associated ESTs. We sequenced one of *F the BDGP cDNAs (GH10034) and it has a contiguous ORF. This high degree of *F similarity is reinforced by conservation of key amino acids required for *F amidophosphoribosyltransferase activity. Therefore, we think Drosophila *F has a second, functional 'Prat' gene. *F .... *F Is it premature to rename CG10078? We haven't got a CG10078 mutant yet *F (the hypomorphic Prat mutants are recessive semi-lethals). I feel the *F sequence conservation is so high (higher than any D.melanogaster-vertebrate *F Prat comparison, which are around 50% identity) that it must encode an *F enzyme with the same-or similar-function. Prat was named based on sequence *F alone, but I'm not sure whether this is all it takes now. *F In the lab, we have been calling CG10078 'Prat2'. I was wondering if I *F could use this name in the paper. One possible alternative suggested by *F the nomenclature rules is to name them by their cytogenetic location (i.e. *F Prat-84E and Prat-65D). *F .... *F Regards, *F Denise *F Denise Clark *F Department of Biology *F University of New Brunswick *F Bag Service 45111 *F Fredericton, NB, E3B 6E1 *F Canada *F email: clarkd@unb.ca *F tel: 506-452-6193 *F fax: 506-453-3583 *F >From rd120@gen.cam.ac.uk Wed Aug 29 10:50:17 2001 *F To: clarkd@unb.ca *F Subject: Re: Prat gene names *F Dear Denise, *F At the moment we have a gene record for CG10078 with the valid symbol *F 'Amidophosphoribosyltransferase' which we seem to have given it on the *F strength of your sequence record AF367369. As a gene symbol *F 'Amidophosphoribosyltransferase' leaves something to be desired! *F At the moment, since no other group has published on CG10078 you can *F change it, and it seems entirely sensible to use the symbol your group *F is accustomed to, namely Prat2 (full name Phosphoribosylamidotransferase 2). *F .... *F Let me know whether you will be settling on Prat and Prat2. If so I *F will curate the sections of your mail regarding the gene names with the *F result that the Amidophosphoribosyltransferase to Prat2 change will *F appear sooner rather than later in FlyBase, which would be a good *F thing. *F All the best, *F Rachel. *F FlyBase Consortium. *F From clarkd@unb.ca Tue Sep 04 13:06:46 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Prat gene names *F Hi Rachel, *F Yes I am happy with Prat2 for CG10078. Go ahead and make the *F change. *F .... *F Thanks very much, *F Denise # *U FBrf0138612 *a Levis *b R. *t 2001.8.30 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Thu Aug 30 18:08:36 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: l(3)s2222 is probably an allele of Takr99D *F The l(3)s2222 P element insertion is in an intron of the Takr99D *F gene, according to the current genome annotation (scaffold *F AE003771.2). It is therefore likely that this mutation is an allele *F of Takr99D and that the l(3)s2222 and Takr99D genes can be merged. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0138613 *a Furlong *b E. *t 2001.9.5 *T personal communication to FlyBase *u *F From furlong@cmgm.stanford.edu Wed Sep 05 00:55:01 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10728 *F Hi Rachel, *F You E-mailed me some time ago about what we were calling a Zinc *F finger protein that I have been working on. We have since named it *F gleeful (gfl) and have some data about this gene in a recent paper *F Patterns of Gene Expression During Drosophila Mesoderm Development. *F Science, Aug 31 2001, 1629-1633. Research Article. Eileen E. M. *F Furlong, Erik C. Andersen, Brian Null, Kevin P. White, and Matthew P. *F Scott. *F The gene name is CG4677 *F Thanks for your help, *F Eileen # *U FBrf0138614 *a Duffy *b J. *t 2001.9.7 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Sep 07 17:15:35 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Act5C(FRT.polyA)lacZ.nls1}3 *F The following information accompanied a stock donated to the Stock Center *F by Joe Duffy, Indiana University (9/01). *F P{Act5C(FRT.polyA)lacZ.nls1}3 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0138615 *a Levis *b R. *t 2001.9.10 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Mon Sep 10 19:47:31 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: BEST:LD11166 = CG3287 *F BEST:LD11166 is listed as a gene in FlyBase. The only listed synonym *F is n(2)09967, which is the name of a P-element insertion allele. *F There is a stock of n(2)09967 aka ms(2)09967 in the Bloomington stock *F center (BL-11776). I noticed on the GeneSeen display that n(2)09967 *F is inserted at the 5' end of CG3287. I did a BLAST of of EST *F LD11166.5prime and found that its 5' end essentially coincides with *F that of CG3287. It would therefore seem that BEST:LD11166 and CG3287 *F are synonyms and should be merged. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ *F From rd120 Thu Sep 13 13:39:05 2001 *F To: flybase-help@morgan.harvard.edu, levis@ciwemb.edu *F Subject: Re: BEST:LD11166 = CG3287 *F Dear Bob, *F Thanks for your mail \- I'll curate it as a pc and that will bring about *F the merge of the two genes, which will become evident on the public *F server in a few weeks. Incidentally, I was unsure whether or not to *F merge since the insertion is not clearly within CG3287 (in fact it is *F clearly outwith CG3287) but LD11166 matches CG3287 perfectly, which is *F good enough reason to merge. *F Best wishes, *F Rachel. *F From levis@ciwemb.edu Mon Sep 17 18:08:12 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: BEST:LD11166 = CG3287 *F Rachel \- You are correct that the insertion in n(2)09967 (aka *F ms(2)09967) is ~30 bp upstream of the 5' end of LD11166 and CG3287. *F This insertion probably shouldn't be listed as an allele of CG3287, *F unless there is additional genetic or molecular data. ...Bob # *U FBrf0138616 *a Perkins *b L. *t 2001.9.13 *T personal communication to FlyBase *u *F From perkins@helix.mgh.harvard.edu Thu Sep 13 19:58:02 2001 *F Subject: Info *F To: *F Dear Flybase, *F Here is some clarifying information on two genes listed in Flybase: *F CIP-61 \- 'Corkscrew Interacting Protein-61' was the name we initially gave *F to the predicted protein encoded by a cDNA that we isolated by its ability *F to bind to the PTP csw. We subsequently named the protein DIM-7 based on *F the phenotype of embryos deleted for the genomic region encoding CIP-61. *F These embryos display 'diminished' nuclear staining of the activated form *F of D-ERK. Mutant alleles of the gene were isolated (in Danny Brower's lab) *F by their ability to suppress wing blisters and the gene ID'd by these *F alleles was named moleskin. So in collaboration with Danny we decided to *F call the gene moleskin and the protein product Dim-7. Interestingly, Dim-7 *F is also the Drosophila homologue of the vertebrate protein Importin-7, a *F member of the Importin superfamily of nuclear import proteins. *F CIP-D2 \- 'Corkscrew Interacting Protein-D2' was the name we initially gave *F to the predicted protein encoded by a cDNA that we isolated by its ability *F to bind to the phosphatase insert of the PTP csw. We have subsequently *F determined that the cDNA that encodes CIP-D2 is an alternative splice *F product of the gene nuclear fallout, Nuf. Therefore, we now call this *F predicted protein NufD2 (with D2 in superscript). *F Gene listed in Flybase as anon-D2 is also NufD2. *F If you have any questions please do not hesitate to contact me. *F Thanks, *F Liz Perkins *F Lizabeth A. Perkins, Ph.D. *F Assistant Professor of Surgery (Genetics) *F Pediatric Surgical Research Laboratories *F Warren 10 *F Massachusetts General Hospital *F Boston, MA 02114 *F Phones: (617) 724-1618 office; (617) 724-1625 lab *F FAX: (617) 726-5057 *F E-mail: perkins@helix.mgh.harvard.edu # *U FBrf0138617 *a Castelli-Gair Hombria *b J. *t 2001.9.19 *T personal communication to FlyBase *u *F From jec24@cam.ac.uk Wed Sep 19 15:47:28 2001 *F To: Rachel Drysdale *F Subject: dome *F Dear Rachel, *F This info relates to domeless (dome). Not all of this will be published *F in our Current Biology paper. *F dome is the computer annotated GADFLY gene CG14226. We isolated the *F dome alleles from the Gottingen X P collection. We named all alleles *F using as superscript the original number used in the Gottingen X P *F collection (i.e.dome367 corresponds to l(1)G0367). By plasmid rescue *F we found that seven elements are inserted 400bp apart in 18E. l(1)G0321 *F is inserted 30bp upstream the start of LD32858 and has no zygotic nor *F maternal cuticle phenotype. The other rescued P elements are inserted *F in the 5' UTR at the following positions with respect to the start of *F LD32858: dome405 is inserted at 20b, dome367 77 b, dome217 and *F dome218 154b, dome468 268 and dome441 376b. We have other dome *F alleles that have not been rescued: l(1)G0199b, l(1)G0264 and *F l(1)G0282. 321, 405 and 367 are hypomorphic insertions, the others have *F strong zygotic domeless phenotypes. Based on the identical phenotypes *F in germ line clones of the strong alleles to germ line clones of *F stat92E null alleles, they may be null. *F There is a further strong allele: dome9 that was isolated from line *F l(1)G0009. This is not caused by the P insertion which is in Act5C as *F the phenotype can be separated from the P insertion. *F We know that all the above mutants are dome alleles because the zygotic *F spiracle phenotypes are rescued by UAS-dome5.1. (see below). *F UAS constructs: The EST LD32858 was obtained from Research Genetics *F from the embryonic 0-22 hours LD library. UAS-dome was made by an *F EcoR1/Xho1 excision from pOT2a and directionally subcloned into *F EcoR1/Sal1 cut pUAST. UAS-dome&Dgr;TMCYT and UAS-dome&Dgr;CYT were *F similarly subcloned but using the Bgl2 and EcoRV sites within dome. *F Several independent lines were tested for each experiment. One of the *F UAS-dome constructs (UAS-dome5.1) rescues the spiracle phenotype even *F in the absence of a GAL4 driver line, suggesting that a nearby enhancer *F activates the construct in the posterior spiracles. *F James # *U FBrf0139552 *a Wilder *b J. *t 2001.9.27 *T personal communication to FlyBase *u *F >From jawilder@princeton.edu Thu Sep 27 22:18:51 2001 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Thu, 27 Sep 2001 22:18:51 +0100 *F From: 'Jason Wilder' *F To: 'Michael Ashburner \(Genetics\)' *F Subject: RE: mini-me *F Date: Thu, 27 Sep 2001 16:18:12 -0400 *F MIME-Version: 1.0 *F Content-Transfer-Encoding: 7bit *F X-Priority: 3 (Normal) *F X-MSMail-Priority: Normal *F X-Mailer: Microsoft Outlook IMO, Build 9.0.2416 (9.0.2911.0) *F Importance: Normal *F X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2600.0000 *F *F Dr. Ashburner, *F *F Thank you for your interest in our article. I am including several *F sequences of mini-me elements from D. melanogaster, as well as their Genbank *F accession numbers below. Regarding the mini-me element in the ciD gene (Acc# *F U66884), it appears to be located from bases 14880-15490, in the first *F intron. A 1360 element also appears in this intron immediately downstream, *F from approximately bases 16335-17000. Based on the presence of mini-me *F elements in other genomic locations, and in other taxa, I believe the two *F elements to be distinct. *F Additional copies of mini-me elements from D. melanogaster can be found in *F the following locations: *F *F 1) Acc#: X01918 (68C Glue cluster) *F Bases: 3961-4304 *F Sequence: *F TTTTTCTACCCGTTACTCGTAGAGTAAAAGGGTATACTCGTTTCGCTGAGAAGTAACAGGCAGAATATAAAGCATA *F TATATTCTTGATTAGGGTCAATAGCCGAGTCGATCTGGCCATGTCCGTCTGATTCTGTTTGCCACTCCCACATTTT *F TGAAAAATGTTTTATAATTTTTTCATATTTTTATTATCTAAATCTATCCCTTCCACACCTTAGAGCATTAAATTTA *F ATTTCTTTCCCCCAATTTTTACCGATATTCGTGAAAAATGTTATACATTTTCCATTTCACTTGAACTAGCTAAGTA *F ACGGGTATCTGTTAGTCTCGTTAGCGTTCTCTCTTGTTTT *F *F 2) Acc#: AF025540 (her gene) *F Bases:2084-2680 *F Sequence: *F TTTATACTCGTTACTCGTAGAGTAAAAGGGTATACTAGATTCGTTGAAAAGTATGTAACATGCAGAAGAAAGCGTT *F TCCGACTATATAAAGTGTATATATTTATATATAAGTATATATTCTTGATCAGAATCAATAGCCGAGTCGATCTAGT *F CATGTCCGTCTGTCCGTATGAACGACGAGATATCAGAAACTTTAAAAGCTAGAAGGTTGAGATTTAGCATACATAT *F TCTAGAGATAAAGACGCAGCGGAACCCATGTTGCGACGCCCACAAACCGCACAAAACTGACACGCCCATATTTAAA *F AAAAAAATTGTTGGATATTTTTCATAATTTTATTAGTCGCTTAAATTTCTATCGATTTGCCAAAAAACTTTTTGCC *F ACGCCCATTCCAACGCCCTAAAGCCGCCAAACCGGTCACGCCCACACGTTTGAATTTTTTTTAAATTTTTTCACAT *F TATACAATATTTATTAATATCCCAGAGGATTAAATTTCGCGTTCGCATTCACACTAGCTGAGTAACGGTTATATGA *F TAGTCGGGGATCTCGACTATAGTATTCTTTCTTTTTTAAACTACTCCTATACTTGTTTTTGGGCT *F *F 3) Acc#: X62679 (su(f) gene) *F Bases:561-1063 *F Another copy: 9086-9589 *F Sequence: *F TTATTATACCCGTTACTCGTAGAGTAACGTAGATTCGTTTAAAGGAATGTAACACGCAGAAGGAAGCGTTTCCGAC *F TGTATAACGGATCAATAGCCGAGTCGATATAGCCATGTCCGTCTGTCCGTATGAACGTCGAGATCTCAGGAACTAT *F AAAAGCTGGAAGGCGGAGATTTAGCATGCAGATTCTAGAGACAAAGACGCAGCGCAAGTTTGTTGATCCATGTTGC *F AACGCCCACAAGCCCACGAAAAAATGCCACAAGCACTCTTTTGAAAAATATATTGATATTTTTTCACATCTTTATT *F AGTCTTGTAAATTCCGATCGATGTGCAAAAAAAAACTGTTTGCCACACCCACTGTGACGCCCTTAAGCCGACAAAC *F CGGTCACGCCCCACTTTTGAAAATTGTTGAAATTTTTTCTCATTTTATTCCCCAACATCTTTTGGTATCGCAGAAA *F AATATCTGGGTTAACGGGTATCTGATATTCAGGCAACTCGACTATAG *F *F Again, thanks for your interest! Please let me know if you have any other *F questions. *F *F Sincerely, *F *F Jason *F *F *F -----Original Message----- *F From: Michael Ashburner (Genetics) [mailto:ma11@gen.cam.ac.uk] *F Sent: Monday, September 24, 2001 4:58 AM *F To: jawilder@Princeton.EDU; ma11@gen.cam.ac.uk *F Cc: gm119@gen.cam.ac.uk *F Subject: Re: mini-me *F *F *F Did you recieve this email ? *F Michael *F *F > From ma11@gen.cam.ac.uk Mon Jul 30 13:59:41 2001 *F > Envelope-to: ma11@gen.cam.ac.uk *F > Delivery-date: Mon, 30 Jul 2001 13:59:41 +0100 *F > To: jawilder@princeton.edu *F > Subject: mini-me *F > Cc: ma11@gen.cam.ac.uk, gm119@gen.cam.ac.uk *F > X-Sun-Charset: US-ASCII *F > From: Michael Ashburner (Genetics) *F > Date: Mon, 30 Jul 2001 13:59:42 +0100 *F > Content-Length: 626 *F > *F > Dear Jason *F > *F > I have been reading yr interesting paper on mini-me in MBE. A nice *F > study ! *F > *F > A question: You give the accession number U66884 as a reference for *F > a melanogaster mini-me; but this seems to be a 1360 element in ci. *F > *F > We maintain a file of 'reference' sequences of melanogaster te's: *F > *F > *F (ftp://ftp.ebi.ac.uk/pub/databases/edgp/sequence_sets/transposon_sequence_se *F t.embl.v4.5) *F > *F > and I would like to enter a clean annotated melanogaster mini-me sequence. *F > Can you supply or point me to one please ? *F > *F > Michael Ashburner *F > *F > (perhaps the one used by you from near hermaphrodite in yr screen of the *F > genome would be most appopriote). *F > *F *F Sequence format is Pearson *F Sequence 1: AF025540 597 bp *F Sequence 2: X62679 503 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 79 *F Guide tree file created: [/net/nfs0/vol1/production/w3nobody/tmp/700053.266393-373259.dnd] *F Start of Multiple Alignment *F There are 1 groups *F Aligning... *F Group 1: Sequences: 2 Score:7001 *F Alignment Score 2428 *F CLUSTAL-Alignment file created [/net/nfs0/vol1/production/w3nobody/tmp/700053.266393-373259.aln] *F *F AF025540 --TTTATACTCGTTACTCGTAGAGTAAAAGGGTATACTAGATTCGTTGAAAAGTATGTAA 58 *F X62679 TTATTATACCCGTTACTCGTAGAGTAACG--------TAGATTCGTTTAAAGGAATGTAA 52 *F \****** \***************** \********** \*** \* \****** *F *F AF025540 CATGCAGAAGAAAGCGTTTCCGACTATATAAAGTGTATATATTTATATATAAGTATATAT 118 *F X62679 CACGCAGAAGGAAGCGTTTCCGACTGTATAACG--------------------------- 85 *F \** \******* \************** \***** \* *F *F AF025540 TCTTGATCAGAATCAATAGCCGAGTCGATCTAGTCATGTCCGTCTGTCCGTATGAACGAC 178 *F X62679 ----------GATCAATAGCCGAGTCGATATAGCCATGTCCGTCTGTCCGTATGAACGTC 135 *F \****************** \*** \************************ \* *F *F AF025540 GAGATATCAGAAACTTTAAAAGCTAGAAGGTTGAGATTTAGCATACATATTCTAGAGATA 238 *F X62679 GAGATCTCAGGAACTATAAAAGCTGGAAGGCGGAGATTTAGCATGCAGATTCTAGAGACA 195 *F \***** \**** \**** \******** \***** \************ \** \********** \* *F *F AF025540 AAGACGCAGCGGAA---------CCCATGTTGCGACGCCCACAAACC-GCACAAAACTGA 288 *F X62679 AAGACGCAGCGCAAGTTTGTTGATCCATGTTGCAACGCCCACAAGCCCACGAAAAAATGC 255 *F \*********** \** \********* \********** \** \* \**** \** *F *F AF025540 CACGCCCATATTTAAAAAAAAAATTGTTGGATATTTTTCATAATTTTATTAGTCGCTTAA 348 *F X62679 CACAAGCACTCTTTTGAAAAATAT-ATTGATATTTTTTCACATCTTTATTAGTCTTGTAA 314 *F \*** \** \** \***** \** \*** \******* \* \********** \*** *F *F AF025540 ATTTCTATCGATTTGCCAAAAAA--CTTTTTGCCACGCCCATTCCAACGCCCTAAAGCCG 406 *F X62679 ATTCCGATCGATGTGCAAAAAAAAACTGTTTGCCACACCCACTGTGACGCCCTTAAGCCG 374 *F \*** \* \****** \*** \****** \** \******** \**** \* \******* \****** *F *F AF025540 CCAAACCGGTCACGCCCACACGTTTGAATTTTTTTTAAATTTTTTCACATTATACAATAT 466 *F X62679 ACAAACCGGTCACGCCC-CACTTTTGAAAATTGTTGAAATTTTTTCTCATTTTA-----T 428 *F \**************** \*** \****** \** \** \********** \**** \** \* *F *F AF025540 TTATTAATATCCCAGAGGATTAAATTTCGCGTTCGCATTCACACTAGCTGAGT-AACGGT 525 *F X62679 TCCCCAACATC-------------TTTTGGTATCGCAGAAAAA-TATCTGGGTTAACGGG 474 *F \* \** \*** \*** \* \***** \* \* \** \*** \** \***** *F *F AF025540 TATATGATAGTCGGGGATCTCGACTATAGTATTCTTTCTTTTTTAAACTACTCCTATACT 585 *F X62679 TATCTGATATTCAGGCAACTCGACTATAG------------------------------- 503 *F \*** \***** \** \** \* \*********** *F *F AF025540 TGTTTTTGGGCT 597 *F X62679 ------------ *F *F =================== *F Sequence format is Pearson *F Sequence 1: X01918 344 bp *F Sequence 2: AF025540 597 bp *F Sequence 3: X62679 503 bp *F Sequence 4: U66884 611 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (1:2) Aligned. Score: 37 *F Sequences (1:3) Aligned. Score: 27 *F Sequences (3:4) Aligned. Score: 62 *F Sequences (1:4) Aligned. Score: 42 *F Sequences (2:3) Aligned. Score: 79 *F Sequences (2:4) Aligned. Score: 71 *F Guide tree file created: [/net/nfs0/vol1/production/w3nobody/tmp/138530.5284-378698.dnd] *F Start of Multiple Alignment *F There are 3 groups *F Aligning... *F Group 1: Sequences: 2 Score:7001 *F Group 2: Sequences: 3 Score:6474 *F Group 3: Sequences: 4 Score:3270 *F Alignment Score 9107 *F CLUSTAL-Alignment file created [/net/nfs0/vol1/production/w3nobody/tmp/138530.5284-378698.aln] *F *F Your Multiple Sequence Alignment: *F *F 138530.5284-378698.aln *F *F *F *F CLUSTAL W (1.81) multiple sequence alignment *F *F *F AF025540 --TTTATACTCGTTACTCGTAGAGTAAAAGGGTATACTAGATTCGTTGAAAAGT-ATGTA 57 *F X62679 TTATTATACCCGTTACTCGTAGAGTAACG--------TAGATTCGTTTAAAGGA-ATGTA 51 *F U66884 ----TATACCCGTTACT---------------------AGATTCGTTGAAATGA-ATGTA 34 *F X01918 TTTTTCTACCCGTTACTCGTAGAGTAAAAGGG-----TATACTCGTTTCGCTGAGAAGTA 55 *F \* \*** \******* \* \* \***** \* \* \*** *F *F AF025540 ACATGCAGAAGAAAGCGTTTCCGACTATATAAAGTGTATATATTTATATATAAGTATATA 117 *F X62679 ACACGCAGAAGGAAGCGTTTCCGACTGTATAACG-------------------------- 85 *F U66884 ACAGGCAGAAGGAAGCGTCTTAGACCATATATAGTAT--------ATACATACATGTATA 86 *F X01918 ACAGGCAGAATATAAAGCATATATATTCTTGATT-------------------------- 89 *F \*** \****** \* \* \* \* *F *F AF025540 TTCTTGATCAGAATCAATAGCCGAGTCGATCTAGTCATGTCCGTCTGTCCGTATGAACGA 177 *F X62679 -----------GATCAATAGCCGAGTCGATATAGCCATGTCCGTCTGTCCGTATGAACGT 134 *F U66884 TTCTTGATCAGGATCAATAGCCGAGTCGATCTTGCCATATCCGTCTGTCCGTATGAACGT 146 *F X01918 ---------AGGGTCAATAGCCGAGTCGATCTGGCCATGTCCGTCTGATTCTGT-----T 135 *F \***************** \* \* \*** \******** \* \* *F *F AF025540 CGAGATATCAGAAACTTTAAAAGCTAGAAGGTTGAGATTTAGCATACATATTCTAGAGAT 237 *F X62679 CGAGATCTCAGGAACTATAAAAGCTGGAAGGCGGAGATTTAGCATGCAGATTCTAGAGAC 194 *F U66884 CGAGATCTCAGGAACTATAAAAGCTAGAAGGTTTAGATTCAGCATACA-------GAGAC 199 *F X01918 TGCCACTCCCACATTTTTGAAAAATGTTTTATAATTTTTTCATATTTTTATTATCTAAAT 195 *F \* \* \* \* \* \* \*** \* \** \** \* \* *F *F AF025540 AAAGACGCAGCGGAA---------CCCATGTTGCGACGCCCACAAACC-GCACAAAACTG 287 *F X62679 AAAGACGCAGCGCAAGTTTGTTGATCCATGTTGCAACGCCCACAAGCCCACGAAAAAATG 254 *F U66884 AAAGACGCAA-GTAGCC------ATGCCCACTCTAACGTCCACAAACA-GCGCAAAACTA 251 *F X01918 CTATCCCTTCCACAC---------CTTAGAGCATTAAATTTAATTTCTTTCCCCCAATTT 246 *F \* \* \* \* \* \* \* \** \* *F *F AF025540 ACACGCCCATATTTAAAAAAAAAATTGTTGGATATTTTTCATAATTTTATTAGTCGCTTA 347 *F X62679 CCACAAGCACTCTTTTGAAAAATAT-ATTGATATTTTTTCACATCTTTATTAGTCTTGTA 313 *F U66884 TCACGCCCACACTTTTGAAAAATGT-GTTG--TTCTTTTCACATTCTGATTAGTCTTTTA 308 *F X01918 TTAC--CGATATTCGTGAAAAATGTTATACATTTTCCATTTCACTTGAACTAGCTAAGTA 304 *F \** \* \* \***** \* \* \* \* \* \*** \** *F *F AF025540 A---ATTTCTATCGATTTGCCAAAAAA--CTTTTTGCCA------------CGCCCA--- 387 *F X62679 A---ATTCCGATCGATGTGCAAAAAAAAACTGTTTGCCA------------CACCCA--- 355 *F U66884 C---ATTTCTATCGATTTCCAAAAAAAAACTTTTTGCCAACGCCCTAAAACCGCCCAAAA 365 *F X01918 ACGGGTATCTGTTAGTCTCGTTAGCGTTCTCTCTTGTTTT-------------------- 344 *F \* \* \* \* \* \* \*** *F *F AF025540 TTCCAACGCCCT---------AAAGCCG---------------CCAAACCGGTCACGCCC 423 *F X62679 CTGTGACGCCCT---------TAAGCCG---------------ACAAACCGGTCACGCCC 391 *F U66884 CTCCGACACCCACATTTGTAAAAAATTGTTGGGAATTTTTTTCATAAATTTATTAGTTTA 425 *F X01918 ------------------------------------------------------------ *F *F *F AF025540 ACACGT-TTGAATTTTT------------------------------TTTAAATTTTTTC 452 *F X62679 -CACTT-TTGAAAATTG------------------------------TTGAAATTTTTTC 419 *F U66884 TTATTTATTATAAATTTAAGTTTATATCGATTTGCCGACAACATATTTTAATTTTTTTTC 485 *F X01918 ------------------------------------------------------------ *F *F *F AF025540 ACATTATACAAT--ATTTATTAATATCCCAGAGGATTAAATTTCGCGTTCGCATTCACAC 510 *F X62679 TCATTTTA-------TTCCCCAACATC-------------TTTTGGTATCGCAGAAAAA- 458 *F U66884 TCATTTTATCTTTTATCTATCGATATCCCAGAAAAAT--TGTGCAATTTCGCATTCACAC 543 *F X01918 ------------------------------------------------------------ *F *F *F AF025540 TAGCTGAGT-AACGGTTATATGATAGTCGGGGATCTCGACTATAGTATTCTTTCTTTTTT 569 *F X62679 TATCTGGGTTAACGGGTATCTGATATTCAGGCAACTCGACTATAG--------------- 503 *F U66884 TAGCTGAGT-AACGGGTATCTGATAGTCGGGAAACTCGACTATAGCATTCTCTCTTTTTG 602 *F X01918 ------------------------------------------------------------ *F *F *F AF025540 AAACTACTCCTATACTTGTTTTTGGGCT 597 *F X62679 ---------------------------- *F U66884 AAATTGCGG------------------- 611 *F X01918 --------------------------- *F ============================= *F Sequence format is Pearson *F Sequence 1: AF025540 597 bp *F Sequence 2: X62679 503 bp *F Sequence 3: U66884 611 bp *F Start of Pairwise alignments *F Aligning... *F Sequences (2:3) Aligned. Score: 62 *F Sequences (1:2) Aligned. Score: 79 *F Sequences (1:3) Aligned. Score: 71 *F Guide tree file created: [/net/nfs0/vol1/production/w3nobody/tmp/993585.295920-372875.dnd] *F Start of Multiple Alignment *F There are 2 groups *F Aligning... *F Group 1: Sequences: 2 Score:7001 *F Group 2: Sequences: 3 Score:6453 *F Alignment Score 6884 *F CLUSTAL-Alignment file created [/net/nfs0/vol1/production/w3nobody/tmp/993585.295920-372875.aln] *F *F Your Multiple Sequence Alignment: *F *F 993585.295920-372875.aln *F *F *F *F CLUSTAL W (1.81) multiple sequence alignment *F *F *F AF025540 --TTTATACTCGTTACTCGTAGAGTAAAAGGGTATACTAGATTCGTTGAAAAGTATGTAA 58 *F X62679 TTATTATACCCGTTACTCGTAGAGTAACG--------TAGATTCGTTTAAAGGAATGTAA 52 *F U66884 ----TATACCCGTTACT---------------------AGATTCGTTGAAATGAATGTAA 35 *F \***** \******* \********* \*** \* \****** *F *F AF025540 CATGCAGAAGAAAGCGTTTCCGACTATATAAAGTGTATATATTTATATATAAGTATATAT 118 *F X62679 CACGCAGAAGGAAGCGTTTCCGACTGTATAACG--------------------------- 85 *F U66884 CAGGCAGAAGGAAGCGTCTTAGACCATATATAGTAT--------ATACATACATGTATAT 87 *F \** \******* \****** \* \*** \**** \* *F *F AF025540 TCTTGATCAGAATCAATAGCCGAGTCGATCTAGTCATGTCCGTCTGTCCGTATGAACGAC 178 *F X62679 ----------GATCAATAGCCGAGTCGATATAGCCATGTCCGTCTGTCCGTATGAACGTC 135 *F U66884 TCTTGATCAGGATCAATAGCCGAGTCGATCTTGCCATATCCGTCTGTCCGTATGAACGTC 147 *F \****************** \* \* \*** \******************** \* *F *F AF025540 GAGATATCAGAAACTTTAAAAGCTAGAAGGTTGAGATTTAGCATACATATTCTAGAGATA 238 *F X62679 GAGATCTCAGGAACTATAAAAGCTGGAAGGCGGAGATTTAGCATGCAGATTCTAGAGACA 195 *F U66884 GAGATCTCAGGAACTATAAAAGCTAGAAGGTTTAGATTCAGCATACA-------GAGACA 200 *F \***** \**** \**** \******** \***** \***** \***** \** \**** \* *F *F AF025540 AAGACGCAGCGGAA---------CCCATGTTGCGACGCCCACAAACC-GCACAAAACTGA 288 *F X62679 AAGACGCAGCGCAAGTTTGTTGATCCATGTTGCAACGCCCACAAGCCCACGAAAAAATGC 255 *F U66884 AAGACGCAA-GTAGCC------ATGCCCACTCTAACGTCCACAAACA-GCGCAAAACTAT 252 *F \******** \* \* \* \* \*** \****** \* \* \**** \* *F *F AF025540 CACGCCCATATTTAAAAAAAAAATTGTTGGATATTTTTCATAATTTTATTAGTCGCTTAA 348 *F X62679 CACAAGCACTCTTTTGAAAAATAT-ATTGATATTTTTTCACATCTTTATTAGTCTTGTAA 314 *F U66884 CACGCCCACACTTTTGAAAAATGT-GTTG--TTCTTTTCACATTCTGATTAGTCTTTTAC 309 *F \*** \** \** \***** \* \*** \****** \* \* \******* \** *F *F AF025540 ATTTCTATCGATTTGCCAAAAAA--CTTTTTGCCA------------CGCCCA---TTCC 391 *F X62679 ATTCCGATCGATGTGCAAAAAAAAACTGTTTGCCA------------CACCCA---CTGT 359 *F U66884 ATTTCTATCGATTTCCAAAAAAAAACTTTTTGCCAACGCCCTAAAACCGCCCAAAACTCC 369 *F \*** \* \****** \* \* \****** \** \******* \* \**** \* *F *F AF025540 AACGCCCT---------AAAGCCG---------------CCAAACCGGTCACGCCCACAC 427 *F X62679 GACGCCCT---------TAAGCCG---------------ACAAACCGGTCACGCCC-CAC 394 *F U66884 GACACCCACATTTGTAAAAAATTGTTGGGAATTTTTTTCATAAATTTATTAGTTTATTAT 429 *F \** \*** \** \* \*** \* \* \* *F *F AF025540 GT-TTGAATTTTT------------------------------TTTAAATTTTTTCACAT 456 *F X62679 TT-TTGAAAATTG------------------------------TTGAAATTTTTTCTCAT 423 *F U66884 TTATTATAAATTTAAGTTTATATCGATTTGCCGACAACATATTTTAATTTTTTTTCTCAT 489 *F \* \** \* \** \** \* \******* \*** *F *F AF025540 TATACAAT--ATTTATTAATATCCCAGAGGATTAAATTTCGCGTTCGCATTCACACTAGC 514 *F X62679 TTTA-------TTCCCCAACATC-------------TTTTGGTATCGCAGAAAAA-TATC 462 *F U66884 TTTATCTTTTATCTATCGATATCCCAGAAAAAT--TGTGCAATTTCGCATTCACACTAGC 547 *F \* \** \* \* \*** \* \***** \* \* \** \* *F *F AF025540 TGAGT-AACGGTTATATGATAGTCGGGGATCTCGACTATAGTATTCTTTCTTTTTTAAAC 573 *F X62679 TGGGTTAACGGGTATCTGATATTCAGGCAACTCGACTATAG------------------- 503 *F U66884 TGAGT-AACGGGTATCTGATAGTCGGGAAACTCGACTATAGCATTCTCTCTTTTTGAAAT 606 *F \** \** \***** \*** \***** \** \** \* \*********** *F *F AF025540 TACTCCTATACTTGTTTTTGGGCT 597 *F X62679 ------------------------ *F U66884 TGCGG------------------- 611 *F # *U FBrf0139843 *a Hultmark *b D. *t 2001.10.24 *T personal communication to FlyBase *u *F Date: Wed, 24 Oct 2001 11:19:34 \+0200 *F To: Gillian Millburn (Genetics) *F From: Dan Hultmark *F Subject: Re: FlyBase query *F Dear Gillian, that construct carries the insert of a CecA1 cDNA, *F cloned into the pP(UAST) vector. *F Best wishes, *F Dan *F >Dear Dr. Hultmark, *F > *F >I am curating your paper for FlyBase: *F > *F >Ekengren et al., 2001, Curr. Biol. 11(18): 1479 *F > *F >and I have a question about one of the constructs you used. You use a *F >'UAS-CecA' construct. FlyBase has a record of 2 'CecA' genes \- CecA1 *F >(corresponding to the gene prediction CG1365) and CecA2 (corresponding *F >to the gene prediction CG1367). Could you tell me which one your 'CecA' *F >corresponds to, so that I can record the information about the UAS *F >construct under the correct gene, *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F \-- *F _____________________________________________________________ *F Dan Hultmark Umea Centre for Molecular Pathogenesis (UCMP) *F By 6L *F Umea University *F S-90 187 Umea, Sweden *F Telephone: \+46-90-785 67 78 *F Fax: \+46-90-77 80 07 *F E-mail: Dan.Hultmark@ucmp.umu.se *F _____________________________________________________________ # *U FBrf0139844 *a Santaren *b J.F. *t 2001.10.24 *T personal communication to FlyBase *u *F To: jfsantaren@cbm.uam.es *F Subject: naming of Jafrac1 and Jafrac2 *F From: Aubrey de Grey *F Date: Tue, 23 Oct 2001 13:25:58 \+0100 *F Dear Dr. Santaren, *F We in FlyBase are interested in why you chose the symbols 'Jafrac1' and *F 'Jafrac2' for the thioredoxin peroxidase genes you reported in EJB last *F year. Could you please tell us? *F Many thanks in advance, *F Aubrey de Grey *F FlyBase *F Date: Wed, 24 Oct 2001 12:10:40 \+0200 *F From: 'J. F. Santaren' *F To: Aubrey de Grey *F Subject: Re: naming of Jafrac1 and Jafrac2 *F Dear Aubrey de Grey, *F The symbols 'Jafrac1' and 'Jafrac2' for the thioredoxin peroxidase genes *F reported in EJB correspond to the initials of the people of my group. *F Your sincerely *F Juan F. Santaren # *U FBrf0139845 *a Schlenke *b T. *t 2001.10.30 *T personal communication to FlyBase *u *F Date: Tue, 30 Oct 2001 14:30:56 \-0800 *F To: flybase-help@morgan.harvard.edu *F From: Todd Schlenke *F Flybase, *F The gene Ser7 is a synonym of CG2045. In Coustau et al.'s original *F paper describing how Ser7 is upregulated during the immune response, *F they reported a portion of the amino acid sequence for Ser7. *F Blasting this amino acid sequence against the genome gave one very *F clear hit, for CG2045. Furthermore, De Gregorio et al. (PNAS *F 98:12590-12595) just showed that CG2045 is upregulated during the *F immune response. *F Todd Schlenke *F \-- *F Todd Schlenke *F Section of Evolution and Ecology *F Storer Hall *F University of California *F Davis, CA 95616 *F 530-754-6361 *F FAX 530-752-1449 # *U FBrf0139846 *a Lengyel *b J. *t 2001.1.4 *T personal communication to FlyBase *u *F Date: Thu, 4 Jan 2001 10:05:28 \-0800 *F To: Gillian Millburn (Genetics) *F From: Judith Lengyel *F Subject: FlyBase query arcUAS *F Dear Gillian: *F You are correct that the arc allele is a replacement of l(2)k11011 with a P *F element containing UAS sequences. This particular P element we obtained *F from John Merriam, who made it himself. I do not think it has been *F published yet. .. *F With best regards, *F Judith *F >Dear Judith, *F > *F >I was just correcting some of our curation in the FlyBase files, when I *F >came across something from your paper: *F > *F >Liu and Lengyel, 2000, Dev. Biol. 221(2): 419--434 *F > *F >In this paper (on page 428) you describe the 'aUAS' allele *F >( == superscript). *F > *F >You state that 'A GAL4 inducible promoter, in which five UAS enhancers *F >are fused upstream to the hsp70 basal promoter, was inserted upstream *F >of the endogenous arc gene by P element replacement. This allele with *F >UAS enhancers upstream of the arc gene was designated aUAS' *F > *F >From this, I think that this allele was made by replacing the P element *F >sequences in 'arcP' (also known as l(2)k11011) with P-element *F >sequences which contain UAS sequences (rather than making a UAS-arc *F >construct in vitro and then injecting it). Is this correct ? (At the *F >moment we have the allele as a UAS-arc construct in our files, but I *F >think that we got it wrong when we first curated the paper). *F > *F >I would be grateful if you could tell me which vector you used to make *F >aUAS, i.e. what is the source of the UAS sequences \- is it pUAST, or *F >P{EP} (described in Rorth, 1996, Proc. Natl. Acad. Sci. USA. 93(22): *F >12418--12422), or is it a different vector ? I need to know this *F >information, so that I can record which type of P-element sequences *F >(containing UAS) are inserted upstream of the arc gene. *F > *F >Any information you give me would be recorded as a personal *F >communication from you to FlyBase, *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F Judith Lengyel *F Department of Molecular, Cell & Developmental Biology *F Box 951606 *F UCLA *F Los Angeles, CA, USA 90095-1606 *F email: jlengyel@ucla.edu *F office:310-825-3298 *F lab:310-825-9625 *F fax:310-206-3987 *F website: http://stratus.lifesci.ucla.edu/mcdbio/Faculty/Lengyel/ # *U FBrf0139847 *a Harrison *b D. *t 2001.11.1 *T personal communication to FlyBase *u FlyBase error report for CG2160 on Thu Nov 1 07:26:36 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 1 Nov 2001 07:26:36 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: DougH@uky.edu *F Subject: FlyBase error report for CG2160 on Thu Nov 1 07:26:36 2001 *F Error report from Doug Harrison (DougH@uky.edu) *F Gene or accession: CG2160 *F Release: 2 *F cDNA or EST error *F Comments: Please note that the full-length cDNA LP02169, corresponding to *F socs44A, has been sequenced and reported to GenBank under the accession number *F AF435923 . A reference for this work will be provided upon publication. # *U FBrf0139848 *a Harrison *b D. *t 2001.11.1 *T personal communication to FlyBase *u FlyBase error report for CG15154 on Thu Nov 1 07:21:06 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 1 Nov 2001 07:21:06 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: DougH@uky.edu *F Subject: FlyBase error report for CG15154 on Thu Nov 1 07:21:06 2001 *F Error report from Doug Harrison (DougH@uky.edu) *F Gene or accession: CG15154 *F Release: 2 *F cDNA or EST error *F Comments: Please note that we have reported the sequence of two different *F full-length cDNAs of socs36E from an embryonic library to GenBank under the *F accession numbers: AF435838 and AF435839 . These two isoforms indicate the *F presence of a large (nearly 10 kb) intron upstream of the predicted CG15154 *F sequence. The encoded proteins of these cDNAs are different at the carboxyl *F terminus. A reference will be provided upon publication of this work. # *U FBrf0139849 *a Gjellesvik *b D.R. *t 2001.10.24 *T personal communication to FlyBase *u FlyBase error report for CG6429 on Wed Oct 24 06:30:01 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 24 Oct 2001 06:30:01 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: dag.rune.gjellesvik@biotec.as *F Subject: FlyBase error report for CG6429 on Wed Oct 24 06:30:01 2001 *F Error report from Dag Rune Gjellesvik (dag.rune.gjellesvik@biotec.as) *F Gene or accession: CG6429 *F Release: 2 *F Gene annotation error *F Gene CG6429 has incorrect exon/intron structure or translation start site. *F Comments: The gene corresponds to EST clone RH62928 in the BDGP EST project, *F and starts at an earlier met in the reading frame # *U FBrf0139850 *a Ayme-Southgate *b A. *t 2001.10.16 *T personal communication to FlyBase *u *F From: 'southgate' *F To: *F Subject: projectin (CG1479) *F Date: Tue, 16 Oct 2001 20:36:19 \-0400 *F here is finally the corrected annotation. The corresponding analysis is in *F press in JMB. I will let you know the exact reference ASAP. *F The attached file is in Word format. It contains in that order; 1) the genomic *F sequence with lower case for introns and upper case for exons; 2) the cDNA *F sequence including all exons; 3) the amino acid sequence. *F If you need coordinates for exon-intron splices and or aa motifs let me know. *F Thank you for your help, *F Agnes ayme-Southgate *F \------------------------------------------------------------------------------ *F -- *F Total genomic sequence from ECORI#2short to 24R4 *F aagagcacctattctttggacctgccagtaatagactaaagctcgtggagTACGCTTCGT *F TTTCGCAGAAGAATTAATTTCGTTCTGTGTTCAATCTGTGTTACTTGTAGCCCAgtacgt *F accaagtgaaaaaaatttttaaacacacctcaaaataaatatttaattaatgaatatatgtttatattctacggctgac *F cacagctatagttgcagtattttttatttaaattcccttctgtcgaccactttgttattttaagggcgatggctcattc *F tacatatgcacactaaatataaatggctacgaataatttaagtttttatgtgttcttatttctaaagtgaagtgaaact *F aaacctaaaatgataattattctagaattgttataattccataattaagaaaaaggatatcgaagtgttttacgcttgt *F agcatattgtaaagaaattgagttcgttttattctacacagatatttcacatctgttcatggcgttgtgtaacacgttg *F ttgttgataaaaatagactttgaaacaaccaaaaaaactgatatgagaaattcgcaaacttaactcagctctttaaaac *F tgttgtatttttattaataggaggcaagcacgttttagataataagcggatatcctctttgtctgattttcccttatac *F cgtttccttttgtttaaataatctatgttgtatccggatatttatatattgccacgaatctatctacgaatatatacat *F atttgtgtgagtttagtattcgaatgttcaggtttatttctcagccggtgactgcgaacggctaaaaatagacatgcaa *F tacatgaattttcgtttattcagtaaaatgttttccatcaacaatatctatgctgaatgaagaaaaggcgatcgaattt *F ccgaattccaaaacttagtgggtcgtgtgcaactctaaatacataaatacgtgtgtatcgttctagtttattgtgattc *F ggtttattttttagttacattgttagataacggaaaatagccaatcatagagtatttaaatttaatatattgggataat *F ttctaaacgccaaaaaaacccttttatttttaaataaaacttactatgccctgccctgaactaatagggccgttggaga *F ttaaaatccaaagaatatttacttgtttgatcttggttaaaaatacacattgaataatctttacaacatgaaatttgta *F tttgtttgtctattgttccaaaacggtatttcagtgtcctgagcaattttttctgtttcattcagCCATAGACTCACAG *F CCGTTGAAGACGAAGCTTAAGGAAAACCCTCAGCATGGGGGTGGCTGAAGATTTCGCTCCCAGCTTTGTTAAAAAGCCA *F CAACTGCATCAAGAAGATGATGGCAATCGATTGATATTTGAGTGCCAGCTATTGTCATCCCCGAAACCGGACATTGAAT *F GGTTTCGAAGTGACAATAAAGTAGTAGAAGATGTCCGAACCAAATTTAAAATTCAACCCGTCGGAGAAAATAAGTATAC *F GGTTGTATTGGAGCTTGACGATGTAGTTGAAACGGATGCCGGACTTTATAAAGTCAAAGCAAAGAACAAGTCGGGCGAA *F GTCTCTGCGTCTATTAATTTAAATTTCACACgtaagtcggccagttttaaatacctttgcaaatgaattagtgttccag *F aaaaaaagattgataaatagcgaagaattcatttccggttttataaagaaacgttctgctcgaaccaaatccatttatt *F agattgagtttggtttctatatatattcggatagccggtacacgtttttatgattgttgcttaatattaatatgaactt *F aatttaaccttacagagggtatattgattttagccatatgcaatgcagcctttttcgatattcgcttcctgcggttgaa *F agtagacttttgtgatagtttcatgctgaaagttttatattttatagataaattttagctgtttgggtatttacatata *F tatcagcgttgcaaacttctgaatgaaatcaatataccctctgctattatttcgtgtacctgtaattttttaatttgca *F attattccaaggatatacatttaatagactcgacttgcagaaacgaacttcttattttatttattttgatggttaatga *F ttcgttgacaatattagatgatttttaaaaattaatattcataacatttgcgtctacaaaaatattaaaataattttaa *F aattttactctaccgtcgtaaaaaactctagCTGCTGACGAACC *F Total cDNA sequence for projectin or bt(CG1479). Nucleotide 1 to 2707 *F represents the erroneous gene CG10285. Nucleotide 2708 to 4489 is the sequence *F previously classified as intergenic. Nucleotide 4490 to the end is the 'old' *F projectin sequence as previously published. *F TACGCTTCGTTTTCGCAGAAGAATTAATTTCGTTCTGTGTTCAATCTGTGTTACTTGTAGCCCACCATAGACTCACAGC *F CGTTGAAGACGAAGCTTAAGGAAAACCCTCAGCATGGGGGTGGCTGAAGATTTCGCTCCCAGCTTTGTTAAAAAGCCAC *F AACTGCATCAAGAAGATGATGGCAATCGATTGATATTTGAGTGCCAGCTATTGTCATCCCCGAAACCGGACATTGAATG *F GTTTCGAAGTGACAATAAAGTAGTAGAAGATGTCCGAACCAAATTTAAAATTCAACCCGTCGGAGAAAATAAGTATACG *F GTTGTATTGGAGCTTGACGATGTAGTTGAAACGGATGCCGGACTTTATAAAGTCAAAGCAAAGAACAAGTCGGGCGAAG *F TCTCTGCGTCTATTAATTTAAATTTCACACATGGTAAGTTTTCACTTCTGGAAAACGAGGGACAAATCGACGGATTCGC *F GCCGACATTCGCGAAAAAACCCGCCATACGACAGGAAGAAGATGGAAAACGATTATTATTCGAGTGCCGCGTTAACGCT *F GATCCGATACCCGCCATTATTTGGTTCCATAATGGAGCTGCTGTCAAGGAATCAGAACGCCATAAGATTACTGTGGACA *F AAGACGTTCATTCATATTTTGCAACACTTGAAATACTAAATGTAACAGTTGAGGATGCTGGAAAATACAAAGTGAATGC *F AAAAAACGAATTGGGCGAAAGCAACGCTACAATAAGCCTAAACTTCGACAGCGACGAAGCCCCCGTGCCGGAAAGCGCA *F GAAGGCATTAAGCCAACTTTTACCGAACGCCCGGTAATTCGTCAAAGCGAAGATGGTGGAAATGTAACGTTCGAGTGTA *F GATGCGTTGGAGATCCCACGCCAACAGTCACATGGTCCCATGGCGAGACTGAACTAAACGAATCAAATCGCTACAAAAT *F GTCTTTGACAATGGATCAAAAACTATATCACATTGCGTGTCTTGAGATTAGCAGTGTGGTATCATCTGACCAGGGTGAA *F TATCGCGCGCAAGCGAAAAACAAGCACGGTTCTGGCGTCGCAACTATTAATCTTAATTTCGAAAGCGGCTCGAAAAAAA *F TTCCGGATGGAAAGTCGCCGCGCTTTCCGAAAAAACCAACGATCCGCCAAGAAGAAGACTTACTCATCATGGAATGCGT *F TTTGGAAGCGCATCCGGTTCCGGATATAGTCTGGTATTGCTCAGAAAAGGAAATCTGTAACAATCAAAGAACGAAAATG *F ACTCGCAAGGCAATAACTAAAGACAGCTACATTCTAACTCTGGAAATCCAAAATCCCACCAAAGAAGATGGCGGAAACT *F ATCGTTGTAACGCAATAAATATGTATGGCGAAAGTAATGCAAACATTGCGCTTAACTTTCAAGGTGCGAGCGATGCTAA *F CGGGTTTGCGCCGTCTTTTATCGAAAAGCCGAGAATCATCCCAAATGAATCAGGCACATTGATCACCATGAAGTGCAAG *F TGCAAGGCCAAGCCCGAACCCACAGTAACTTGGTACAGGGGTCAAGACTTGGTGGAGAAGAGTAAGAAGATAAAAATTA *F ACACAACAGTTATTGCAGAAGACACGTATGAGCTGACACTTGAAATTAAGGATCCGGGAGCAACCGATGGTGGGACCTA *F CAGATGCAATGTCAAAAACGAATACGGTGAATCAAACGCAAATCTAAACCTTAACATTGAAGCGGAACCGGAGCCCGAA *F GGAGAGGGGCCAACATTCATAGAAAAGCCACGCATTGTTTCTGAGAACAACGGCAAATTGGTTATAATGGAGTGCAAGG *F TCAAGGCAGATCCGAAACCAGATGTCATTTGGTTTCGCAACGGAGAAGTCATCAAGGAAAGTAACAAAATTAAGACGTT *F TATTGAACAGCGTGGCGACCAATACTACATCAAGCTGGAGCTATTAGATCCGCAACTAGAGGACTCCGGCCTTTACAAG *F TGTAACATTAAGAACACGCTGGGCGAACTTAATGCCAACCTTACCCTGAATATAGAGATCGTTCCTGTTATAAAGGATA *F AGCCAAAAATTATAAAGATTATAAAAAAACGTACTGTAGTCATAGAGTGCACAGTAGCATCAAAATTCGAACCCAAGTG *F TACCTGGTACAAGGAAACCAGCACTGTAAAAGAGAGCAAGAGAC *F ACAATTAACAAAGGCAAATGCAACGGAGCAGTTCTCTCCCACAAAAGCAGTCAAGGCACAAGTCGCTGACTTGAAGAAG *F CCTGAAACTTTAGCAACCCTTGAGGATAACTATGAGCGGCCCGTTTTAGAAAAATATGATCCTTTTAGCATTGATAAGA *F CCAAATCAGAGAAATCCACACCATCCATTATCACCCCGGACATTAGAGGTCCCGAAGTAAAGTTGCCTGAGGAGAAGCA *F AAAGGTCCCTAAAATGCAACCACCTGCTCCAGGTGACCCACCCAAGATCGAAGTGATTCGGGAGAAGCGACCATCACTG *F GCACCAGAGCCCCCAAGTCGCCGCGGATCTCTTATACCCCCCGCTGATACTGGTCGACGACCATCTCTGATCATCAATG *F ATGAGAAGAAACTTCGTCCTGGGGAAGTAATGGACACGCGGTTATTGAGGCCAGGCGAGGTCGGTGAAGGACAGCGGCG *F CAGACCGTCGATTGATGTACGCCGCCCAAGCGTGCAAGATCTAGAAGATCTTATTAACAAGCCATCCACACCCTTACGC *F GATGTCGGCGATGGCGGTCCTCCTTCTATCGTTGATGTCCAAGAGTCTTACTCTGTTGTGGAAGACTCGACTGCTTACC *F TCACTGTTGGCGTCGAAGGTAGTCCAGCACCTACGTTCAAGTTCTACAAGGGAGTTAGCGAGATTCTTGAGGGCGGTCG *F GTTTAAGTTCCTCACAGATGGGCAAACAAACACAATTACATTGTGTATGCGTAAGTGCAAGCCCAATGATGAAAGCAAG *F TACAAGATAGTTGTTTCGAACATTCACGGTGAGGATTCAGCCGAAATGCAACTCTATGTATCTGATTCAAGTGGTATGG *F ACTTCCGTGCCATGCTCAAAAAACGTCGTTATCAAAAATGGGACAAAGACGAACAGGACCCCAATTGGGGCGACCTTAA *F AGAAACAGAAAAACCGTTGCCGGCCCTTAAAAAAGTTGAAAGGAAGGTTGAGTCGTTCCTCAGTCCACTTATCGATCAA *F TTTGCAAAAGAGGGAAAGGACAAAAAGGTTGTTTTTGAAGCCAGATTCAGCAAACCCAATTGCAAGCCCAAGTGGCTGT *F TTCGAAAAGACGAGGTGTTTACCGGCAGTAAATTTAAATTTAAACAAGAAAATGATACTTATCAGTTGATTATTACGAC *F ACCGAAAGTCGAGGACACCGGCAAGTACACGATTGAAATCGGCGGTGTTTCAAGTACAGCATTTTTGAATGTCGAAGAA *F GCCGATCCCACCTACACTTTTACGAAACCCCTTAAAAAGAAACTTGAGGGATTCACTCAGCATGAGACCACTCTTGAGT *F GCTCCGTCAGCAGCAGCATGGCTAATGTGCATTGGTTTAAGAATAACACCAAATTGGAGTCCGATGATCCTCGCTATCT *F TATCTCCAAAGATATAAACGGTAACCTCAAGCTTATTATTAAGGATTCTGTGCTCGATGATGCGGGGCTCTACAGATGT *F CAACTGGATAAGCAACCTGATAAGACTGAGTGTAACCTTAAGGTCACAGAGTACCCGTACAAGTTCGTCAAGGTTCTGA *F AATCACAACAGTGTATTGAGAAAGACACAGTGACGCTCGCCTGCGAAATCGATGATGCTATGGGTGAAGTGCAGTGGTT *F ACGTAACGGCGAAGAAATCAAGCCCGATAAACGTATTCAAATTGTAAAAGATGGTCGAAAGCGAAAGCTAGTCATCAAG *F GACTGCAAGGTTACCGATGCCGGCCAATTCAAATGTACAACGAATGCCGACACCACAGAGTCGGAAATTATTATTAACT *F ATCAAAACCGATTTAATAAAAAACTCAAGGATACTGAAGCTGTTGAGAGAGAAAAATTGATTCTGGACATCGAGCTTCA *F GGATCAAACAGCACCGTGTGACTGGAAATTTAACGGAGAGCCCATAGTACCGAGTGGAAGTATTGAAATCAAGAACATG *F GGTGGCGGCAAGCATCAACTCATATTTAGCAGCCTGGATATGTCTAATGAGGGCGAAATAACTTTCGAGTCTGGACAGC *F TAAGTTCGAAATGCAAGCTTTCTATTCGCAAGGGAGAAAGTAGACCAAACATAGACTGTCCTGATAAATTTTCTGGTCC *F TATCAGTGCCCCTGTGCTTCTAGAAGTGCCATTTAAAGTTTCCG *F Complete amino acid sequence of projectin (CG1479) *F MGVAEDFAPSFVKKPQLHQEDDGNRLIFECQLLSSPKPDIEWFRSDNKVVEDVRTKFKIQPVGENKYTVVLELDDVVE *F TDAGLYKVKAKNKSGEVSASINLNFTHGKFSLLENEGQIDGFAPTFAKKPAIRQEEDGKRLLFECRVNADPIPAIIWFH *F NGAAVKESERHKITVDKDVHSYFATLEILNVTVEDAGKYKVNAKNELGESNATISLNFDSDEAPVPESAEGIKPTFTER *F PVIRQSEDGGNVTFECRCVGDPTPTVTWSHGETELNESNRYKMSLTMDQKLYHIACLEISSVVSSDQGEYRAQAKNKHG *F SGVATINLNFESGSKKIPDGKSPRFPKKPTIRQEEDLLIMECVLEAHPVPDIVWYCSEKEICNNQRTKMTRKAITKDSY *F ILTLEIQNPTKEDGGNYRCNAINMYGESNANIALNFQGASDANGFAPSFIEKPRIIPNESGTLITMKCKCKAKPEPTVT *F WYRGQDLVEKSKKIKINTTVIAEDTYELTLEIKDPGATDGGTYRCNVKNEYGESNANLNLNIEAEPEPEGEGPTFIEKP *F RIVSENNGKLVIMECKVKADPKPDVIWFRNGEVIKESNKIKTFIEQRGDQYYIKLELLDPQLEDSGLYKCNIKNTLGEL *F NANLTLNIEIVPVIKDKPKIIKIIKKRTVVIECTVASKFEPKCTWYKETSTVKESKRHVYQVEQTKEGEFAVKLEINDV *F EESDKGAYKLVASNEKGEAVSQIVNLVDIPEEERKPCKPEISRKLADQKVAESKTFELLVSLSQTDRKCKVEWYKGSTV *F IRETKDITTTFDGTTARLTFSSARTEHTSNYKVIVTNEVGKDESSCKITVEKVAKKKEEKPKEKEKTKNEKEVEQKEME *F EDKNESGQSVAQTEGRINIEQISEGDPKEELTVKEEILDKRDTQEVKESSVELQDSAGHEVPEPKKATSDSKLDQSNQK *F NLDKKHKTDQSESKTNKNVSLAEPIKSNKQESEEQQATEQIGLKKVDRKASIVSVKEEISSDVRRKSTIKAKEEITVDD *F KKASSRRSSLAVEESNTESRRSSIIDKKPLEQVDNKPIDANKNPQPLKEEIPRLKPAEKRRTSKVIEEPKPDEGLPKLR *F KASIAQVKEEAKPAAPKLKAKAKAKPKYEELPEIPDYERPQLEKYEKSDFTPSDFARDLEIPNKMEKPIIDSGKKEPAV *F LAQKNGIPKKTDIIEQYADEPKGLKVGKGKLPDEGDGRDGAVLKPVIIEPEKEILDLGNKKNNQHADKPTVLDIIKQRR *F RSSIRNLMTKEPIQNESFLGVVLKPVIKDTREQAAPQQAIQLTKANATEQFSPTKAVKAQVADLKKPETLATLEDNYER *F PVLEKYDPFSIDKTKSEKSTPSIITPDIRGPEVKLPEEKQKVPKMQPPAPGDPPKIEVIREKRPSLAPEPPSRRGSLIP *F PADTGRRPSLIINDEKKLRPGEVMDTRLLRPGEVGEGQRRRPSIDVRRPSVQDLEDLINKPSTPLRDVGDGGPPSIVDV *F QESYSVVEDSTAYLTVGVEGSPAPTFKFYKGVSEILEGGRFKFLTDGQTNTITLCMRKCKPNDESKYKIVVSNIHGEDS *F AEMQLYVSDSSGMDFRAMLKKRRYQKWDKDEQDPNWGDLKETEKPLPALKKVERKVESFLSPLIDQFAKEGKDKKVVFE *F ARFSKPNCKPKWLFRKDEVFTGSKFKFKQENDTYQLIITTPKVEDTGKYTIEIGGVSSTAFLNVEEADPTYTFTKPLKK *F KLEGFTQHETTLECSVSSSMANVHWFKNNTKLESDDPRYLISKDINGNLKLIIKDSVLDDAGLYRCQLDKQPDKTECNL *F KVTEYPYKFVKVLKSQQCIEKDTVTLACEIDDAMGEVQWLRNGEEIKPDKRIQIVKDGRKRKLVIKDCKVTDAGQFKCT *F TNADTTESEIIINYQNRFNKKLKDTEAVEREKLILDIELQDQTAPCDWKFNGEPIVPSGSIEIKNMGGGKHQLIFSSLD *F MSNEGEITFESGQLSSKCKLSIRKGESRPNIDCPDKFSGPISAPVLLEVPFKVSGTKQTPVEAKLFKDGKPLPVKDVEV *F AVTDDKVTFKIKKPSRDLSGPYQIKISNGQGEDTKDVQIICQDVPQPPQDVDITDVYQTSCVVSFNPPSDDGGTPITKY *F VIERQDLSKKHGWESVAEVLPSEPCLKKIDDLIPKKQYRFRIRA *F \------------------------------------------------------------------------------ *F -- # *U FBrf0139851 *a Deitcher *b D. *t 2001.10.21 *T personal communication to FlyBase *u *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 21 Oct 2001 08:21:53 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: dld14@cornell.edu *F Subject: FlyBase error report for CG17884 on Sun Oct 21 08:21:53 2001 *F Error report from David Deitcher (dld14@cornell.edu) *F Gene or accession: CG17884 *F Release: 2 *F Missed gene *F Comments: This is not to correct an error but rather to add mapping *F information. The map position of the SNAP-25 gene is in the heterochromatic *F region on the 3L side of the centromere. Df(3L)1-16* removes the gene and we *F have several mutant alleles of SNAP-25 that fail to complement this *F deficiency. A more proximal deficiency, Df(3L)8A-80 does not remove SNAP-25. *F We have an in press manuscript describing the isolation of a SNAP-25 mutation *F \*Marchant,G.E. and Holm,D.G. (1988) Genetic Analysis of the Heterochromatin *F of Chromosome 3 in Drosophila melanogaster. I. Products of Compound-Autosome *F Detachment. Genetics, 120, 503-517. *F \**Rao, S., Stewart, B.A., Rivlin, P., Vilinsky, I., Watson, B.O., Lang, C., *F Boulianne, G., Salpeter, M., and *F Deitcher, D.L.(2001) Two Distinct Effects on Neurotransmission in a *F Temperature-Sensitive SNAP-25 Mutant, EMBO, in press. # *U FBrf0139855 *a McGill Drosophila Genome Project *b ?. *t 2001.9.25 *T personal communication to FlyBase *u *F FASTA sequences of Gene Predictions of the 38 region taken from the *F McGill Drosophila Genome Project web page *F (http://ww2.mcgill.ca/Biology/labs/MDGP/home.html) on 25.9.2001. *F >38B.1 *F MAIISQCGCSSSICCVPRNQMTKPWQRRTKWKNINKEDRDNNRLCHFYAKQQTENYIHFL *F LPASRSRTVVTKAAALSVWECRTKFGQITNILRGRHHYLGDIAQLIRHSSKANKKASPEN *F DDKSLSILTNVPNVAQSRGTFHRPNAHLLARQIPNFHTNAGVVGRTPPGNWPTLPSNKSL *F SGGHFRDIIVSLAKGRSGQNGAGKGKAKVNSIKAWTDRSEKRWMAVNCKW *F >38B.2 *F MFHLKRELSQGCALALICLVSLQMQQPAQAEVSSAQGEHLVQPPPEKQSSKDSFLGTPLS *F NLYDNLLQREYAGPVVFPNHQVERKAQRSPSLRLRFGRSDPDMLNSIVEKRWFGDVNQKP *F IRSPSLRLRFGRRDPSLPQMRRTAYDDLLERELTLNSQQQQQQLGTEPDSDLGADYDGLY *F ERVVRKPQRLRWGRSVPQFEANNADNEQIERSQWYNSLLNSDKMRRMLVALQQQYEIPEN *F VASYANDEDTDTDLNNDTSEFQREVRKPMRLRWGRSTGKAPSEQKVCMNIRSFFCAQTKV *F VLLPT *F >38B.3=Barren *F MTLPRLETPLRRSAVGSYQEGVSRMLTPFNDDEAERREARRRTLLQQHHRSSTLESIEDN *F ETIKNCLELYNGNKVSKDNAWNLMLIDSLANLLDHHHKRMSNFKMAGSSLEASSKVYGLR *F VDSIYLDAMRISAGLSARTLTDKQINAAEDDDGPQGEQATGEGQDSAQQAAKEAAPKPKR *F QKKPISTVTKNRETLNSRLDTAPLQDPVFGKLNSTVGSINASNRLMHNILPSFDSELRLR *F TTYNFWNSEESTEEVQDHTTLNAEMEQWPATSLMSTNLMRKLLPHAERSNLRPLHTGYII *F TSAPNPKSANEKAAEVVQDEDHDEGLDNADDMCVNEISMAFDINAECEPMPDLDGPPPLV *F LEVDSNELEELTAEEQMVINNCRRLRKQTEFIEDLRPVDGNSKLEYSYRPMDQISQFWAG *F PSHWKFKRTRPRSTFSQTNGQVDTQPIRTQRAKKSAHLNANRRAKALDYGNVTENFFQQL *F DTTIRQRKANFQKKWDPRKLILPTKFELDPDLFFKYESAPSIKLSKRAGEPDSDEGGDLG *F IDMDADMHHDDDNDQELFNNEHFTDAVPANVSVIAAIAAEQAAEASMMNVSAGEIGLTQM *F NATCNNTVFEIGTEFEGAPSQVAKVIVPFAKRAKVIDMKNLKKSCNSLIQKQLLNAVPEE *F TIPSHPKKKGEHYSKGFASFQQVYQKLPDLLTTKMSDSLSPSVAFYAVLHLANDLKLRLI *F PQEDLEDFQIRQVLD *F >38B.4=loki *F MARDTQGTQGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEAN *F DLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRIL *F KNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTC *F QQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVL *F EFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLETNDEE *F TLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCL *F SGTLPFSDEYGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQ *F SSWLRDAPMLQKAKRLMKLDGMEIEEENFLEPPTKRSRR *F >38B.5 *F MFPQRSSELYRTPVTHQPPPALELAGNLEYPNLNIADLNARLENLSPRLHDCWDSIAIND *F HNHLALATNRREGRQWWGMLFGYGRDQMHHMSVDSANFKLQAEHTVNIVRYAEDDFLLVA *F LGDTRLQAWSTYSKVRDSQSPYCLFLVGESSAHPTPISQLSVFKADPRTAVSGSADSTLN *F VCIKVMWMYGKYTNVTLLQVWDLSGADMVSTYRSRSSHTDKLTGLATPAASVDKFVTCDR *F GGCARLWDVRAAAPSSTCLYADASHVLSFTSAAWAAASELQGDNHIYLGDYDGKVHTLDI *F RVPRKLAETREYFDKGHVAQLLINGYKFKRNNDDLH *F >38B.6 *F MGGGGLLDIYAMAWEHLRPGSSPVDWCEGNYLISSNIAEFVNTFSNFLFILLPPVLIMLF *F KEYGRFVTPGIHVIWVLLIVVGLSSMYFHATLSLIGQLLDELAILWVFMAAFSLFYPKRY *F YPKFVKNDRKTFSWLMLLSAIAATGLSWWKPIVNAFVLMFMSVPTMVMLYTELQR *F >38B.7 *F MTLSRKDFQALLIVICFGFWIENLVQGADDYYVYSPVCQMPAVNPFTWDTMSNFHRKTLR *F HCHNDSDMVTSEFDFETHQYRLHVHEHLAKPIMNRKGNATLECEYQEISRDNSDQKPDNA *F YNQLRRRPIEQHQFVPNNTDYMITSCYVRDVTKHRELLQQDAISFIEDRLPKEKVKDFVK *F DESEVHKPSVLIMGLDSTSRINLRRAMPSVYKFVRQPGWFEMQGYNKVGDNTFPNLLAAL *F TGDSEKGVGDYCDVTKPGCLDSLNFIWKRFKKANYTTAFAEDCSSISTFNYLKPGFVKQP *F TDYYLRPLLFAIEKQFKVTNDFGFAYCVGRHLSFSYVWDFGQQFIDRFLGRSPMFGFLWS *F NSFTHDYYEGATALDNLLWKYLKSFEESNLFQKSIVILMSDHGHRYNTLRRASTGYFEER *F MPMMFIYLPPWFRRKYPHLASNLGKNQNRLSSNYDVYMTLQHLLQLDSKSVDEFPDNLRA *F RQCKSCQSLFFELPFNRTCQMAGIEEKWCCCQPTETITNSPHVSTIAEAIVQRMNEHLIS *F HNLSDLCHNFTLDYVEKADRKTILSNGLRPADKNEQVYIIVFETVPKNPIFEATVRWNSR *F TQRLLHFDVEELSRLTSYKNDANCINRKNAKKYCICKDSLSRPS *F >38B.8 *F MANKLFQLYKKCALQLRCRNFTIIVYGFKACTILSLGLWSLLCQLSIPAQSSLDGVAQSL *F TWIPDRNEGYPELELVLWPVLQQRLSLGQLRFHVEVGEKHQHRVGGYKNEYVPEAVQVWK *F IDRGPGLAEDPVIDPAEHRNSPDGRRSDAQAVDPLVTNSV *F >38B.9=purple *F MSQQPVAFLTRRETFSACHRLHSPQLSDAENLEVFGKCNNFHGHGHNYTVEITVRGPIDR *F RTGMVLNITELKEAIETVIMKRLDHKNLDKDVEYFANTPSTTENLAVYIWDNIRLQLKKP *F ELLYEVKIHETPKNIISYRGPYPLNGIYNPINKRIAHDSCTNISSDSD *F >38B.10=KLP38B=Mothra=nebbish *F MSNKSTPVRQRIQQFQTRGVHKSTPASEMRKKVLMTRAEDREHRDRPEQLLNTAFSGSTP *F QPKPKPTALNACYTPSSLYRNANSTPGRAKTPGTGKSSCSRTKERDSLMESCLSVSEESN *F MIVAVRVRPLNALECTRGQVTNVVQVHGNSNELTVQAGSSADASAGVTHFFSYDQVYYSC *F DPERKNFACQAKVFEGTARPLIDTAFEGYNACLFAYGQTGSGKSYSMMGIEALDDAALDG *F GPPHDEAGIIPRFCHELFRRIEAVKSQQQLQVEVEVSYFEIYNEKIHDLLSVQHAAAATG *F ESTPIQQQQQQQRPALKVREHPIFGPYVVDLSAHSVDSYSALRNWLAVGNSQRATASTAM *F NDKSSRSHSIFNIVLNLTDLSSDDGLSSDTDSSTASSLRQTRRSKISLVDLAGSERISVS *F GSNGERIREGVSINKSLLTLGKVIAALADSRKAIANGPLGSGTPSTFVPYRESVLTWLLR *F VSVTK *F >38B.11 *F MLASTAEVISRVANQCAPSESFTRDLQQNTSNFGERFAAQEEERRKTSASHLHAKVHQKA *F LLKKQNKVILKARFIGRPSQSRDKVKRPRTTRAKREKERKTHTHITNTIHIPLIGTAVRK *F NSNETPHSTDMDVWGFCVSDTTMAYGGSMRSGYYRDYEEFPYGTLESTTSSHAVKDCYSR *F VQEKCKQIKSEKQMRKDCPFCKEHKKRPLINYMRKREQRKHHDSICEDEEDMHEHEHELE *F HTHNHSQSTVLAAPQSCKMDWATTIWCVSSLRWLRESPTDLRRHSKKYLSSPFMAKAAYG *F NKSCEPFYGPYYRNEKQSGHWTLNNTQWNGFREKRQVFGLSIKSLSLVGAM *F >38B.12=KLP38B=Mothra=nebbish *F MHHRAFNITDVVPSFQENLGGNSKTVMLATISPASIHADETLATLRYACKARSIVNRVKV *F NESPHDKIIRDLRAEVDRLKSLRNEYERQRRLSGNSNNPVPRKIIIETSVDETEVEALRQ *F QLAERERELSRAQKSWMEKLKEAEDQRKSELRVLKRRGLALELTAEQKQACLVNLTADPI *F LSGTLFYLLPQGLVRIGRGRLPGGSSSSQPDIVLDGPLVALQHCSIEHERGGKLYVIPGS *F EDFETYVNGELLKDRRQLFHGDRLVIGGSHYFRISNPFCSQRGKADHPVDFQLAHQEILQ *F KQEQQLRSELEAEKRAALTKIEQERAQHARDFEERLQCLELEQFKYKCNSEMLETERQAL *F ALAQQQEHTPLRHEDAVSTPAQKSTILEDIQRIMLNPSEESLHKTQLMVKEATQRCRQLD *F LPLEFRQTQTPDEFGLLRTVILILDKQRGLKAEWPTARLGVWLDLVRDNAEQQEKLNATT *F IFQSVEVDWEPLDADLNETLSDTHNSSRIALNLSAMKDVLLNKPLKRLLNASSSKSNTPR *F QTSTPSPGSQLVHFTKRLLTYDPEEIPESQSSFTTLVQQELQIMQRSAQRLRRHCEAALL *F ERENHQDNGVLAVSLQEALARMDTVLNGMHTSLARAEATACVTSPTKTQKAVRFLID *F >38B.13 *F MSKDHWMRDLPSELRDLSIINLAIPGSHNSMTYGINSKSELSPDAEISIRRWHRFFPCFV *F RRWSKTQSSGTLDQLELGVRYFDLRIAQKDDKFYYCHGLFAMEIFEPLLEIRQFVDTHPE *F EVVILDLQHFYAMTVAHHQKLHKDLIQFFAHRLYSTVDGSLKDCTLNRCLEMQRSVVIIY *F RRCPIPLPLRFWPSYAWPTPWPNKASVKKLQSFLEDSLLSRQPQQGYVSQCLITPTGRYI *F AFRLFFTLKSTAKRVDKKLQPWIQEQIPGPFEPKDEPRVNVFLADFVSLKGGQFCDWVVD *F LNTKLLEQLAGDKELESTEGVEEEEAQG *F >38B.14 *F MSRKCEFLVVGGGIAGVSCAESLAIYRPNASILLLTESSIVKSVTNLVPVARYLHKFDVR *F EQDVSEMGASFQTLVDRLDHINSREHCIRTKAGLEIKYRYLCLCTGGTPKLFSGKVVNPL *F VIGIRDTDSVQLLQRKLATAKDVLILGNGGIASELAYELKDVNVHWVVKDSHISATFVDP *F GAAEFFHIAMNECNAKDSSPVVAIKRMRYSEVLPKEQTNNHGAALGPDWHRSVDLSGARE *F GEENRLPKIYYKSRISSVQDLADDAGAIVKLEHEDGSFQQLTCDFIVSATGVWPNTDYTC *F DSPLQFSDDGGISVDEMMRTNLVDVFAAGDVCTANWPAAMHWFQMRLWTQARQMGSMAGR *F SMAAASEGESVYQDFCFELFGHVTKLFGYPVVLLGRFNGQDLGRDYEILVRCTRNKEYIK *F FVLQNGRLRGAMLIGNTDLAETCENLILNGIDLEPYGDDILNPDIDIEDYFD *F >38B.15 *F MASNSMPEENFEGGDFNFDDDAEDDQFVATNSGRKRLFSKELRCMMFGFGDDKNPYTETV *F DLLEDLVIEYIAETTHRAMEIGRTGRVQVEDIIFLVRKDPRKYARVKDLLTMNEELKKAR *F KAFDEIKYVGEYCKSSTFPKNYM *F >38B.16 *F MEVFTFEKSYLERLKEAEAVLSWEGAVMPASQVRSEWKSYVELKIEPAGWQAIWKIPRVI *F CEDLKLRYPTIVYGYVEQVIFDELKAVFVVTAVQDSDVHLPESNEVSLVELWPTVQQENA *F ALNVDTTAECIDRLRFFYTHVWMPWDKDYDDDRDWVQQHLQARIQLVCDLSKNRLPRPLA *F LHMRTLLAEAKYIQQRLDYLELDLSDAESDDEAVELNDSVAEPARKQSKAGSANGCLNVS *F SLPVTDLMCLHLRMAIIRSEFEILENPEMRRAYSELQSNSLKRLRCSSGRTRQSEDLLVE *F RNSISHVVTLPGKLQQQLELLKLAQTLVKPESKVQLSNTLQDVLSICQSNDDILLSPGEH *F TIKFLEHLNDNGSLRGLTQPEAILSPAADLSLLPVVCSSDEDSTLLVIDGDYTLSELVLD *F CRHVRRGILLRNGTLTMRGCRLLGDGSSSTQEGIVCMPGASVELKSCLIENFAVGVSMRP *F KSSAELGSVQFKTCKTGLELLEKSASVNLQGSKCSFENCALGILADGFVLGEQRTEKVLV *F LNKFSELQRYNEDNLLGNCSFYNCKKNVRVFNESGQLLAQRSHQQLLEDELGGENKENIQ *F LV *F >38B.17 *F MLPIARGIYIGLVNITRNSRGALHTASALRCKAETSTEENNAAATEGEGEDNASGSAPDP *F KDRSKPIPVETSIRYLKSAAYKQTYGEDFVWTQYRRNHKGMYAPRKTRKTCIRQNRISTG *F NPCPICRDEYLVLDYRNTELLEQFISPHSGDVLSYSKTGLCQKSHLRLMVAVQQARDSGY *F LTYDVPFREYDYSEYYGQEQKQKA *F >38B.18 *F MAETKPQMASQTNGKGRHTRWHPYTRDFGGMNENGCAVRFGFPVDVLELNRHITGPVLVS *F YEAPGFGNISGNEYMGTRVSTGWGFGAAGGNDINERRNMLGSLTRGHRLELRDGNQILWP *F DIEFSHCIEFNQSGTVIKASQLFSPLDSCSKLLHPQTMAAKSDMDILANSMYEDDPNGNL *F APATTKDKESVKPEELKVTPAGTKATSPPNSATLVSTSPRWGPQNKGAQELALLYSPGKR *F AQEIICVYTCIGLMIINLALIVRHLRLERISVAFLSALCGIITADFASGLVHWAADTWGS *F VDIPMIGKNFLRPFREHHLDPTSITRHDFIETNGDNFMVGIPILGYLAHYFYIRTPSEIQ *F QHFGWIAYVFLCSIFVAMTNQQIDSNGYNMYAMYSIRFWSTFELIIEHFTGLKPRDDDLK *F WAKKLT *F >38B.19 *F MPSLNHRPMLTTDGNNNGQDGSHVRKYDGEGDESNDSDDGNRLQDPMQTEQDITIWNANG *F LINNKPEDELYCKTNQADILSIFQTKERQPGKPEANLGFYRAGNPLQNTRKSIFAQSVAS *F TGRCRSDPQFGFRRCHGTPEQCHRLVKRILETFERKQYCCAIMLDIKPAFDKVWHPGLHF *F KIKMNLPSSHFAFLKSFTEVDINRPECQKWMVQVYSKCRDLFKTNTLPHWGLDNAPVVYK *F ADVEAMTVLDCHRLKRALGKEVSEEDEEGDELDDLDDLDEGEEGLDPGRLVGLLLALCAI *F ALLFVLLCRMKRRNIKLKEVELPKPEPQEAPSVLPQKKKLNRAANCKSWMRRSCRPFFLH *F NESQLRQYQARAEMEVAVHEERTRQKIAMWTKARERDAQKKRDKLQRNVNKATKT *F >38B.20 *F MAAILMIHKPGKPELSTKSYRPISLLSSPSKVWKRLIANRLGDIINECRVLPDHQLGFRQ *F GHSTVEQAFDDKEYCNAVFIDMQQAFDSVWHDGLLSKIKRLFPAPYYGVLRSYLEDRKFM *F VRARNSYSTPCVMGARVPQGSVLGQLLYSVFTADLPCPNAYHMADPRKALLATYADDIAL *F LYSSNWCNEAARGIQEYLTTLAAWCKRWNLNVNPQKSTNPCFTLKTLSPATAPIELEGVI *F LDQPLQAKYLGITLDKRLTFGLEAKPKQSSTSSRSNSTTETAEAAADKGQREIHSSSRRT *F PATTSKPSSTSPIMMPLRILVWNADGVSTKLPEVECFVRRHEIDVLLLNETHCKRTETPK *F LFGFVAYTANDPSGGNAKGGAAILIKSSLAHFPLTPIATDKEQPKRYCTSEPRPKLGGGL *F ASNRRLATRSISRQPAADRIPITKEILLLIARRGAYVLERLANTGVESEATHSLWKATRA *F IKRRCTRKAPLEDSNGTWCRTDLGQAEVFAAHLAERFQPFKLASLQQVEETQDLLNQALQ *F MDLPITPFEPCEVAEVIVRQSSNKAPGHDVICNATLKALPSQAILYITLVFNAIVRLQYF *F PYQWKLINKPGKPEREPASYRPISLLPSILKVFERLIAVRIMEAQGITPEHQFGFRAGHC *F TVEQLHRVVEQILTAYDNKEYCNSLFLDIREAFDRVWHIGLQVKIKQTLPAPYFGLLKSY *F LEGRRFAVRFHSAISTEHNVAAGVPQGSLLGPLLHCLYSHDMPQPDVSLYEKSMLATFAD *F DVCVTYRSRYEHDAADGIQDFADRFSEWARRWNIGINSSKFNNVCYTLKRTATARLHRGS *F PSTTAERSKFKIPPPEGVNQFRKAPTVGASGCSFIMDWQAVPIATSIKRKKKEKKNSSSS *F DSYDTLEPVKVKRKNLVKHSHPIDGPTTPTHHQAALADNSFAILSSDDDDDEMFDDLPAK *F TPLQEKEMEKPKAIKPPPIFIPDVTNIKALVNSIAGVLGKDVEFHLIRQATTTFA *F >38B.21 *F MNQGKYLDVLNNHAFPSGDKLIGESFILQQDNAPCHKAKLITKFLKDVGVNTLDWPPQTT *F SEIAVAIAEIVVVVAPEAILHQEICAVDYCLLVVEVIVASADGRLMVHPNRLANILVNPI *F SLRRLKRVHPSDLPTRRIREGGVGGFIRRFHGTPEQCHRLVARILDAFENNRYCSAVFLD *F VKQAFDRVWHPGLLYKLKSHLPSSHYALLKSYTEGREFQVRCGSSTSTTRPIRAGVPQGS *F VLGPILYTLFTADLHIIPSRYLTAATYADDTALIATATNPQRASAIIQRQLDALDPWLKR *F WNIVINADKKNSDSMKLPIKPRG *F >38B.22 *F MLIVGVEPRILNLNDIKIVARDAFGIVNFVFRNMKIDFDIPDKWLLLYLCQRQDFTKFLI *F DVTEIEDVVLDTAFSAGKFTSSDRCRLRISPAPSTIAIHQWRLPCATPFGCARNAPEQEW *F RFTFHPSVKQRKSYFSTFKKLGRCIQWKASKSAPFRRTLFITEKVNKTRILPIDHIVTIV *F HIRVLPLPSVISGKETVPRLGNIAKEGSAIFIKANIRHSQNKSTMRTDLQVTSLDVPTSG *F SIIKIASLYLPPNKPWTKNDFDQLLITLGPKCIAGGDYNAKLQWWANLRTCSRGKRLQEA *F IVTSSSEVIATDLYENSRIDCFLLQECRCVRAYLCEHDIHHSPAASGFSAEEGANLHTHT *F RCTQAADDQVMSLPQYIAIAGYSGA *F >38B.23 *F CCQLYYHAATTIIVYSRPSCSSSTLLSLSSFAFRKNRTKMITKMTMHDKESKREI *F >38C.1 *F MFKHYPKNREATAKANDCHLATLGIERSGSRGKKTRRKGIQSICHNFRQWLIKTKNKKQN *F QHNRGKSTLSQDLLKSNGTHSSVTVEDDEHGDVDAAASVLRGTAKRCIPGGAVESYDRMA *F THSHTHTRILAPFLQGVHPFQSGLFLAAAVSIKIQVRCSTVAVIVRFASLINGIQAHSSG *F KDMQNRSKDSRGQRSRSPLTDKRDTQGDTKICEKAKAKPHSKRHDGRL *F >38C.2 *F MVKMSWDMIALIVDKHNEYRNKFAGGMDQNPKAARMTTIEWDPELAKVADGLVRRCEPIR *F DQCAITPNYGHAEVSYSLEKYFCMTTKKEALRKQLDHWFDPNSKDEVQKLFFSWTKNQQK *F SKILLKTVSRELSKNYFQVLRDRANRVGCAIVEYVRPALVHQLLKCVYNCGVSLCEEEDN *F PVYEDTDEEAASECMKGSNKQYKNLCHKDELVKTCNGGSLFVEPENDYNDGQEENMENDY *F EFETTVFTLPTYDPNDVLPTLPDNPDLNFNTIVKD *F >38C.3 *F MTPNAIYPYKLQPEKRGNAAPAAAAFAVKFQRVSVCRLNVCRILLEKGGQTTNKVVTKSL *F TVWHLHRNAIFGQKTHTTETGEAVAKVLPPSCAASLRRQAHIIGARVNVLLRNVQFRPHL *F VVPTRTPPKEKTRRIHCVRCWDYRSGVSSVSAVKSEIPGHAETEEAAECMETEMETQHLG *F KYFT *F >38C.4 *F MGKTKRVVGLTLKEKLQIIELVTNKVDKKEICAKFKCDRSTVNRILQKTNEIHEAVVASG *F LKRKRQRKGAHDLVEEALYIWFGQQESKNVILDRHVILAKAKEFCQKFNDAFEPDASWLW *F RWRKRHNIKYGKIHGETATNDSVSANEYKNDILPGLLKGYNPEDIFNADETALFYKAMPN *F ATFFTCGKQLNGQKSQRVRLTLLFICNATGTYKKTFVIGRSKSPRCFKNANVPIPYYANK *F KAWMTKDLWRKIMTGFDEEMKKQNRKILLFIDNATSHTTVKDFENIKLCFMPPNTTALLQ *F PLDQGIIHSFKLEYRRILVKQQLIAVNCGKSTVEFLKSLSLLDALYFVNQGWKNVKMLTI *F QNCFKKAGFKFSFENEDTIAEKDKQCVEVDIVSNINWNEYANVDADEACHGQLDDDEIVR *F SLVQDAKTSDNEESHSDEDVDDTERPTFKDGFAAIKALKKTKEPLGIFFANLEPASNNKE *F VFQIRILCRSVVTIEPPLEFNNVPQCFRCQGFGHTQRYCFLEHRCVKCGGHHDTRTCAKK *F EDEKACCLHYQADHPASFRGHRRKDDGENVLANYAASCFHPQRKSMQSLKIVLWNANGLQ *F RSRDEVKHLLKTDAIDVLLQTVTSTQNTPGGVHSINPKGRTLHRFLQSRRLDCHSSGEPT *F HWLTNPALLPDLLDFAISKGIGQARLTCSKELKDSYVPDGKTPSTDSLRSWNLGILSKIL *F ERVFLRRVLPVLYEAGLIPDHQFGFRRSHGTPEQCHRLVEQILEALERKQYCCAVMLDVK *F QAFDKVYKIKTHLTGSHFAFLKSFTEGREIQVRCGTATSTPRPIRAGVPQGSVLGPILYT *F LYTADLPITPSRSLTVATYADDTAFLASASDPQEPSAIIQSQLDALDPWLKRWNIAVNAD *F KSSQTTFSLRRGDCPPVMLNGETILTSSSPKYLGLTLDRRLT *F >38C.5 *F MGKTKRVVGLTLKEKLQIIELVTNKVDKKEICAKFKCDRSTVNRILQKTNEIHEAVVASG *F LKRKRQRKGAHDLVEEALYIWFGQQESKNVILDRHVILAKAKEFCQKFNDAFEPDASWLW *F RFNSQFNCLLGMDWAHNLL *F >38C.6 *F MGKTKRVVGLTLKEKLQIIELVTNKVDKKEICAKFKCDRSTVNRILQKTNEIHEAVVASG *F LKRKRQRKGAHDLVEEALYIWFGQQESKNVILDRHVILAKAKEFCQKFNDAFEPDASWLW *F RFNSQFNCLLGMDWAHNLL *F >38C.7 *F MDKAKRNIKPFDGEKYAIWKFRIRALLAEQDVLKVVDGLMPNEVDDSWKKAERCAKSTII *F EYLSDSFLNFATSDITARQILENLDAVYERKSLASQLALRKRLLSLKLSSEMSLLSHFHI *F FDELISELLAAGAKIEEMDKISHLLITLPSCYDGIITAIETLSEENLTLAFVKNRLLDQE *F IKIKNDHNDTSKKVMNAIVHNNNNTYKNNLFKNRVTKPKKIFKGNSKYKVKCHHCGREGH *F IKKDCFHYKRILNNKNKENEKQVQTATSHGIAFMVKEVNNTSVMDNCGFVLDSGASDHLI *F NDESLYTDSVEVVPPLKIAVAKQGEFIYATKRGIVRLRNDHEITLEDVLFCKEAAGNLMS *F VKRLQEAGMSIEFDKSGVTISKNGLMVVKNSGMLNNVPVINFQAYSINAKHKNNFRLWHE *F RFGHISDGKLLEIKRKNMFSDQSLLNNLELSCEICEPCLNGKQARLPFKQLKDKTHIKRP *F LFVVHSDVCGPITPVTLDDKNYFVIFVDQFTHYCVTYLIKYKSDVFSMFQDFVAKSEAHF *F NLKVVYLYIDNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSERMIRTITEKARTMV *F SGAKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYLKHLRVFGATVYVH *F IKNKQGKFDDKSFKSIFVGYEPNGFKLWDAVNEKFIVARDVVVDETNMVNSRAVKFETVF *F LKDKFPNESKECDNIQFLKDSKESENKNFPNDSRKIIQTEFPNESKECDNIQFLKDSKES *F ENKNFPNDSRKIIQTEFPNESKECDNIQFLKDSKESNKYFLNESKKRKRDDHLNESKGSG *F NPNESRESETAEHLKEIGIDNPTKNDGIEIINRRSERLKTKPQISYNEEDNSLNKVVLNA *F HTIFNDVPNSFDEIQYRDDKSSWEEAINTELNAHKINNTWTITKRPENKNIVDSRWVFSV *F KYNELGNPIRYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLAIQYNLKVHQMDV *F KTAFLNGTLKEEIYMRLPQGISCNSDNVCKLNKAIYGLKQAARCWFEVFEQALKEFETQI *F GRHTSRYSDRLLSHSSLLARLLIPARPLRRLKRQGFAKAIGQQ *F >38C.8 *F MTATVHKILVHSKDILEQTVFPVGYFGEEAAESRNTIYQLDRHHHARKHNRICSFADLVQ *F RAMDTSDSIISSINLKGRIRKQNRLPLPPEVIQMLAYEFEPDNDSLDSADCDEEVLYNIK *F MEN *F >38C.9 *F MSWTAYRDIKVERNSDGEFDLEPVGFCPNTTNSTAAMDAAQLQAIIAGAVNQALTEQENR *F LKRDFQAQLDEVKQQMQHLRVEAPQVETYQKITAGPEVRCDIKLDIVKTMPDFSGEQDDY *F VSWRQSAVDAYEIFKRYNGSEAHYQAVSIIRNKIRGPARGLLVAHNTVLNFDAIIARLDC *F TYADKTSLRVLRQGLDTVRQGELSLMQYYDDVEKRLTLITNKIVMTHEPDSAILFNNEVR *F EDALHAFIAGLRKPLRAIVLPAQPKDLPSALALARESEATIERTNFAATYAKALEDKAIT *F YEHRKDRNHSREPHGRYSRAEDSQGKNPHFYKKQGRQNNSAQNNNSYRNQSPEPMELGST *F VTQHRQPTSFGSGQPASVKAEGARGQKRFGSARMTGQRRQRVQNTIQQADDKNDSEYEGA *F AAAAVTEIDDEERSRLPFIERQFHGRKLRILIDTGAAKNYIKPVKELKNVVPVNSPFTVS *F SIHGSNTVKQKCLVNIFGKTADFFLLDTLTLFDGIIGFDFLAQVGAKLDTEKGTINYGSV *F VEELQHHSCDEVNFTNVNDATVPESVKTEFTAMIANRSKAFSASNEALPFNTSVVATIRT *F SDDKPVYSKLYPHPMGVSEFVKREIADLLQKGIIRTSRSPYNNPTWVVDKKGHDEQGNKN *F KRLVIDFRKLNEKTIADKYPMPNIPMILANLGKAKYFTTLDLKSGYHQIYLAEHDREKTS *F FSVNGGKYEFCRLPFGLKNAGSIFQRAIDDILREQIGKSCYVYVDDVIIFSENENDHVKH *F IDWVLKSLCDANMKVSNEKTHFFKQSVEYLGFIVTNGGAKTDPEKVKAIKEYPEPTNLYE *F LRSFLGLASYYRCFVKDFAAIARPLTSLMKGENGSISKHMSRKTPIEFGDLQRDAFERLR *F NVLASEDVILRYPDFRKPFDLTTDASANGIGAVLSQDKRPITMISRTLKESESHYATNER *F ELLAIVWALGKLQHYLYGTRDINIYTDHQPLTFAVSDRNPNPKIKRWKAYIDDHNAKIHY *F KPGKDNHVADALSRQNINALQSEPPSDAATIHSELSLTYTVEATDQPVNCFRNQIVLEEA *F RFRLKRSLVLFRSKTRHLINFADKSTILEMLKEVVNPDVVNAIHCDLPTLASFQHDLIVH *F FPATQFRYCKNIVIDVTNRNEQLEIVTTEHNRAHRAAQENTKQILRDYYFPKMSSLAKEV *F VANCKICTKAKYDRHPKKQELGETPIPSYTGEMLHIDIFSTNKKQFLTCIDKFSKFAIVQ *F PVLSRTIVDVTGPLLQLVNLFPKIKTIYCDNEAAFNSETITSLLKNSYHIDIVNAAPLHS *F SSNGQVERFHSTLSEIARCLKLDKKISDTTELILRATIEYNKTLHSVTQEKPVEILHSGP *F DDRCLGIKDKLVKAQKNNIERCNPARQNRVFEVGDEVFVKNNRRFALVTLIILAVANARI *F TDFSHAKYIPVVDGDVLVFEHRNCLRHSSNLSDYIYMVDETKKLSASFPQSHMRKLLDVD *F TDHLVNLLSVLKIHHRIARSLDFLGTALKVVAGTPDASDFLKIKMTEAQLVDSNSRQINI *F NSETQIQINKLTDTVNRIIKARNNDLVDTPHLVKVKCKKVLVYPVSHYQTILRLDEDTLA *F ECEEDTFSVTECVETTHDTFCERSRRDTCARSLHAGNTAQCHTQSNHLRAVMPIDDGIVI *F INEATARVSTDGGPEVLVKGTHLITFEQSATINGTEFVNLRKAINKQPGVARSPLLNIVG *F HDPELSMPLLHRMNNDNLRFIQGFKDEVDAAGSPKLWFVAGVVLNVGLIGSLILFLALRK *F RRASAEIQQTIDKLNITEDGHNLRGGKWLEALGCQTSGTKAELIVRLQAISPETRGDSPP *F NNGSAAEELEELDGAAAWPKQREPQGGDTRTIPFDEQSLQSEPPENLDLDQLSLVDAERA *F TLKKLRDEIEANMAVLQRIQADIDDANKSKSAHVRQINDVIENNSNGNHGNVNICANSTD *F ANVNSTKVTIDYGTYQCWKRNRW *F >38C.10 *F MSWTAYRDIKVERNSDGEFDLEPVGFCPNTTNSTAAMDAAQLQAIIAGAVNQALTEQENR *F LKRDFQAQLDEVKQQMQHLRVEAPQVETYQKITAGPEVRCDIKLDIVKTMPDFSGEQDDY *F VSWRQSAVDAYEIFKRYNGSEAHYQAVSIIRNKIRGPARGLLVAHNTVLNFDAIIARLDC *F TYADKTSLRVLRQGLDTVRQGELSLMQYYDDVEKRLTLITNKIVMTHEPDSAILFNNEVR *F EDALHAFIAGLRKPLRAIVLPAQPKDLPSALALARESEATIERTNFAATYAKALEDKAIT *F YEHRKDRNHSREPHGRYSRAEDSQGKNPHFYKKQGRQNNSAQNNNSYRNQSPEPMELGST *F VTQHRQPTSFGSGQPASVKAEGARGQKRFGSARMTGQRRQRVQNTIQQADDKNDSEYEGA *F AAAAVTEIDDEERSRLPFIERQFHGRKLRILIDTGAAKNYIKPVKELKNVVPVNSPFTVS *F SIHGSNTVKQKCLVNIFGKTADFFLLDTLTLFDGIIGFDFLAQVGAKLDTEKGTINYGSV *F VEELQHHSCDEVNFTNVNDATVPESVKTEFTAMIANRSKAFSASNEALPFNTSVVATIRT *F SDDKPVYSKLYPHPMGVSEFVKREIADLLQKGIIRTSRSPYNNPTWVVDKKGHDEQGNKN *F KRLVIDFRKLNEKTIADKYPMPNIPMILANLGKAKYFTTLDLKSGYHQIYLAEHDREKTS *F FSVNGGKYEFCRLPFGLKNAGSIFQRAIDDILREQIGKSCYVYVDDVIIFSENENDHVKH *F IDWVLKSLCDANMKVSNEKTHFFKQSVEYLGFIVTNGGAKTDPEKVKAIKEYPEPTNLYE *F LRSFLGLASYYRCFVKDFAAIARPLTSLMKGENGSISKHMSRKTPIEFGDLQRDAFERLR *F NVLASEDVILRYPDFRKPFDLTTDASANGIGAVLSQDKRPITMISRTLKESESHYATNER *F ELLAIVWALGKLQHYLYGTRDINIYTDHQPLTFAVSDRNPNPKIKRWKAYIDDHNAKIHY *F KPGKDNHVADALSRQNINALQSEPPSDAATIHSELSLTYTVEATDQPVNCFRNQIVLEEA *F RFRLKRSLVLFRSKTRHLINFADKSTILEMLKEVVNPDVVNAIHCDLPTLASFQHDLIVH *F FPATQFRYCKNIVIDVTNRNEQLEIVTTEHNRAHRAAQENTKQILRDYYFPKMSSLAKEV *F VANCKICTKAKYDRHPKKQELGETPIPSYTGEMLHIDIFSTNKKQFLTCIDKFSKFAIVQ *F PVLSRTIVDVTGPLLQLVNLFPKIKTIYCDNEAAFNSETITSLLKNSYHIDIVNAAPLHS *F SSNGQVERFHSTLSEIARCLKLDKKISDTTELILRATIEYNKTLHSVTQEKPVEILHSGP *F DDRCLGIKDKLVKAQKNNIERCNPARQNRVFEVGDEVFVKNNRRFALVTLIILAVANARI *F TDFSHAKYIPVVDGDVLVFEHRNCLRHSSNLSDYIYMVDETKKLSASFPQSHMRKLLDVD *F TDHLVNLLSVLKIHHRIARSLDFLGTALKVVAGTPDASDFLKIKMTEAQLVDSNSRQINI *F NSETQIQINKLTDTVNRIIKARNNDLVDTPHLVKVKCKKVLVYPVSHYQTILRLDEDTLA *F ECEEDTFSVTECVETTHDTFCERSRRDTCARSLHAGNTAQCHTQSNHLRAVMPIDDGIVI *F INEATARVSTDGGPEVLVKGTHLITFEQSATINGTEFVNLRKAINKQPGVARSPLLNIVG *F HDPELSMPLLHRMNNDNLRFIQGFKDEVDAAGSPKLWFVAGVVLNVGLIGSLILFLALRK *F RRASAEIQQTIDKLNITEDGHNLRGGVVNN *F >38C.11 *F MIVCFFVCFVGLMSVRPTTFVTPLGQFEFLRMPMGLKTASAVFQRFVNKIFEDLIRDNKV *F IAYMDDIMIASKNSEEHLETLKEVFIRLAQNKLELRMDKCEFLQTNIKYLGFIINGEGIR *F ADDKGIDAIEHFPVPDKIQAVQSFLGLCSYFRRFIKDFSTLAKPLYDLLRKDEKFKFGEK *F EMSCFSTLKEKLVQAPVLAIYNFKDEIELHCDASALGFGAVLCKKKEDGKLHPIFYFSKR *F TTVAEAKYHSFELETMAIIHALRRFTIYLYGKRFKIITDCNSFTLTLNKIELNPRIARWA *F LELQNYDYELIHREGKRMQHVDALSRCTNILIVESNTFEDNLVICQGKDQKVQEIKKLLE *F KNEHNLFEMRNGIIYRKCNNGRLLFYVPTDMEDHILYKYHNEIGHIGRDKMLDVISKSYW *F FPNAKDKCLSHIENCLKCVAFSPKTGKEGFLHCIPKGSKPFVLIHIDHYGTVDSGRSKKH *F IFVVIDGFTKFVRLYATKTTNTKEVITALKKYFRAYN *F >38C.12 *F MHEDCRPFSAVTGAGFKNLVKFFLKIGAVYGDQIDVDDLLPDPTTLSRKAKSDAEEKRSL *F ISSGIKTAVDSGGASATVNMWTDEHVQRNFLAITFHYGKEFKLCDMILGLKSINFQNSTA *F KNILMKIKGLFSEFNVENIDNVKFVTDRGANIKKAQAV *F >38C.13 *F MIGARDVEYRIDSGARTTGCLSACADLYCAFVIEIGTDKKEALNAVSKWTVGACPPFSAV *F TGSGFKSMVKFFIKIGSTYGELVDIDELIPNPTSIA *F >38C.14 *F MRAYRVVIKGLHHTADRAEIQEELEKLTHKQQQGGIPDEDTVQVTIEPPLEFNNVPQCFR *F CQGFGHTQRYCFLEHRCVKCGGHHDTRTCAKKEDEKACCLHYKADHLASFRGHSITETHL *F QEFFETFGPRFIAAGDFNAKHSWWSPLHQPQRQNAPQIPAEQKTGLPLLWHRASQTNLLQ *F GNSKTSYVTYGKTPSTDSLRSWNLVTRNTICGMSRVISGGLQRRRHRVLPEIVQKSFSSS *F NLENLKPILPHIDRYTVRPSSWMTSRRSTECGLLYKLKSNLPSSHYAILKSYTEGREFQV *F RCGSSTSTTRPIRAGEPQGSVLGPILYTLFSTDLPSIPSRYPTAATYEDDTAFLATATNP *F QRASAIIQRQLDPWLKR *F >38C.15 *F MTATVHKILVHSKDILEQTVFPVGYFGEEAAESRNTIYQLDRHHHARKHNRICSFADLVQ *F RAMDTSDSIISSINLKGRIRKQNRLPLPPEVIQMLAYEFEPDNDSLDSADCDEEVLYNIK *F MEN *F >38C.16 *F MQSLKIVIWNANGLQRSRAEVEHLLKTDAIDVLLVSETHFCPRSHFSISGYDVIPANHPS *F GRARGGAAMLIRSGIQYTELPAFQEEWAQCALARISSLHGDITVGAVYFPPRHSITETHL *F QDFFESFGPRFIAAGDFNAKHSWWGSRSINPKGRTLHRFLQSRRLDCHSSGEPTHWPSNP *F SLLPDLLDFAISKGIGQDRLTCSNYNKLISDHSAIKMLLNIPVLKKELPRRLTGKHTDAS *F KFTLWMLSSLHPDPPLSTPSNIDAAIKTLTDEMYNAAEFANPPPSTSPRTPGRDLHLWSP *F EIAAFVAEKRRLRRIWFFSRNPRDKAALNRAIKELKDKLCTLRQDSFDRFLEELEPGDPQ *F HNLWQVTRHIRRPAKKASPVRKADGSWCRSEAERAEAFADLQNAFTPFDRCTGEERAATT *F RFLESPCPPSLPIEPVTPEEVAQEIASLKASKSPGLDRIDATSLKMLPPPCSQLLANIYN *F RCFSLGYFPRSWKRAEVILILKPGKPEANLASYRPISLLTILSKILERVFLRRVMPVLDE *F AGLIPDHQFGFRRSHGTPEQCHRLVARILDAFENKRYCSAVFLDVKQAFDRVWHPGLLYK *F LKSHLPSSHYALLKSYTEGREFQVRCGSSTSTTRPIRAGVPQGSVLGPILYTLFTADLPI *F IPSRYLTAATYADDTAFLATATNPQLASAIIQRQLDALDPWLKRWNIMINADKSSHTTFS *F LRRGECPPVSLDGDTIPTSSTPKYLGLTLDRRLTWGPHINNKRIQANIRLKQLHWLIGKK *F SKLREKLKILVYKTILKPIWTYGIQLWGTACTSHRRKIQRFQNRCLRIVSNAHPYHENSA *F IHEELGIPWVDDEIYRHSVRYASRLENHHNHLAVNLLDHSQSLRRLQRTHPLDLTQHT *F >38C.17 *F MLLKEKRAQIQDGMFAKNCLLRAYITETGTTFSLRSGECPPVTLDGDTITTTSTPNYLGL *F TLDRRLTWGPHINRKRIQANIRLKQLHWLIGKKSKRPSLPRNSAIHEELGIPWVDDEIYR *F HSASNQESKKTEEIDKKIVNFELFIKKKLPECNRQLDRFISKHTKWMASKITIVVSDFEN *F SNIPQQMGRRNLSYQNAGERLKRKLASNLASESDHDTSLLIHAATVSARKECKRDVAFVL *F KETLKTPEFPSGCKFNAKNQLLFHQMKL *F >38C.18 *F MPDDKYVVRHSATSATTEDSRVINGWPFKSSFVVLRRQLNKLLRVISLTFVAGISFIVIL *F TRAFAQIEWNNLPPAINSFAIKVNLHELKGSEL *F >38C.19 *F MGKGGGWVWLWRMPKECRERVAGCCLNYAKFAPTPAEMGTRTNNIIVDVPPFCTGNFIIS *F RPSPSKATATFTPSLSSFAAVAVTLPAIFGYF *F >38C.20 *F MEGRWRTGRQASAAASPKVWRHEHAKGKDTGRQGHPSAAGQHLQQVVYGFSPSPVSYGST *F SATFLPIFGLVKLNYYAKSTMSICATKKVKATARRGAHWSSTAGELDADAEWGLEVPHFS *F RTFSELLLRVSMRNDGSGSGFGFGFWGKGPNFVSEKLRRSLLQKFITVQMLPVLVNRAAE *F RGSEGTTGRKDERKGAGNAFKSSLSFELFNYKVRKMFAYFSAVCDTDEEQFSVFSFQQLA *F VFRGPVFPHLRLLAVIALFASCHTAQLVHVALHFARFLGLGFGRVRGGGALDWDIVSYSE *F KRLSGSGRVKLVTKLLAKTSSTVRGRPKCKSRSGRTAAARSA *F >38C.21 *F MTTPATTDKNNDNNTSSNTNNNSSNKKSNESRQQQRDMARTTTMAAGKASGKMREENIWH *F TLRRAKSDKMWRNESKKSESDAEASPEVEFYAAKSAVWHFERPPSRKSRSFEHQHRLKHS *F >38C.22 *F MNRLLLSVVALVALAASCHGNPGLDFPFGRIVNGEDVDIENYPYQVSVQTTKGSHFCGGS *F LIDSETVLTAAHCMQSYAASELQVRVGSTSRSSGGEVVTVRAFKYHEGYNSKLMINDVAI *F IKLSSPVRQTSKIRAIELADSEAVSGTNAVVSGWGTTCFLFCSSPDTLQKVEVDLLHYKD *F CAADTYNYGSDSILETMVCATGEKKDACQGDSGGPLVADNKLVGVVSWGSGCAWTGYPGV *F YADVASLRSWIVDTTDSL *F >38C.23 *F MPETAAICSANRAVTKNREQNKALSYHTDAKANFPFTASMRPLFLLRVQLMCTDLFRYRL *F LECQRRQVQDPEIGRDGELVVQSQGLS *F >38C.24 *F MPNIVALKLSASYPLMPGHKDEGNILALDQLALQQQISANFIFHAISNYKKTPSERKTLV *F FIAKKWLHIQMLWREFRLRDKQIRRLGHKDARVLEHGYFQQELFELVHEKYMVVRGFLSR *F DKRNLLAAIPFPFPNANSNPNPNPNPYPSNNNPNPMGNQSDWTLDAIYAQSQ *F >38C.25 *F MQKSDYPRTFHTRPQGVWRSGPPSGVCKCAYGTWLMMGHCEERRGWDPSGWLRKWRGRWA *F EWHHNANDICLKEWQPESGYSHFMVADFGSATFGGGSQCQCVRTGAYQAYRPIIPR *F >38C.26 *F MRICGQEPPSVGGSSSVAGVGAWPGRCEHVFLPVECSWWPLIEMHVVGLAEERGSWIQTA *F AEALTGSRKRLKVFSVSRWGKEQAYQTAMLPSGFRCPRAAAIAAGRAKVAAGAGTNEKGH *F RVGVEFNFQKKKKTLKTSARPAAVAGRMEKSKINEARAQCNQWLKPEHNRVAATIKQHQK *F HDQQLQQLVKQPGHFTQIEIGRKIALS *F >38C.27 *F MDKAKRNIKPFDGEKYAIWKFRIRALLAEQDVLKVVDGLMPNEVDDSWKKAERCAKSTII *F EYLSDSFLNFATSDITARQILENLDAVYERKSLASQLALRKRLLSLKLSSEMSLLSHFHI *F FDELISELLAAGAKIEEMDKISHLLITLPSCYDGIITAIETLSEENLTLAFVKNRLLDQE *F IKIKNDHNDTSKKVMNAIVHNNNNTYKNNLFKNRVTKPKKIFKGNSKYKVKCHHCGREGH *F IKKDCFHYKRILNNKNKENEKQVQTATSHGIAFMVKEVNNTSVMDNCGFVLDSGASDHLI *F NDESLYTDSVEVVPPLKIAVAKQGEFIYATKRGIVRLRNDHEITLEDVLFCKEAAGNLMS *F VKRLQEAGMSIEFDKSGVTISKNGLMVVKNSGMLNNVPVINFQAYSINAKHKNNFRLWHE *F RFGHISDGKLLEIKRKNMFSDQSLLNNLELSCEICEPCLNGKQARLPFKQLKDKTHIKRP *F LFVVHSDVCGPITPVTLDDKNYFVIFVDQFTHYCVTYLIKYKSDVFSMFQDFVAKSEAHF *F NLKVVYLYIDNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSERMIRTITEKARTMV *F SGAKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYEMWHNKKPYLKHLRVFGATVYVH *F IKNKQGKFDDKSFKSIFVGYEPNGFKLWDAVNEKFIVARDVVVDETNMVNSRAVKFETVF *F LKDSKESENKNFPNDSRKIIQTEFLNESKECDNIQFLKDSKESENKNFPNDSRKIIQTEF *F PNESKECDNIQFLKDSKESNKYFLNESKKRKRDDHLNESKGSGNPNESRESETAEHLKEI *F GIDNPTKNDGIEIINRRSERLKTKPQISYNEEDNSLNKVVLNAHTIFNDVPNSFDEIQYR *F DDKSSWEEAINTELNVHKINNTWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVA *F RGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLNGTLKEEIYMRL *F PQGISCNSDNVCKLNKAIYGLKQAARCWFEVFEQALKEYLTTAVNILSRYSSKNNSELWQ *F NLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRKSTTGYLFKMFDFNLI *F CWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLENPIKIYEDNQGCISIA *F NNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTKPLPAARFVELRDKLG *F LLQDDQSNAE *F >38C.28 *F MDDKIILNDFSLTTLKDWLRILGQNTEGTKTELIARLQDIPTAVRGDCPPDAPPGNDIFS *F SLDFQNCEINTDHVSVNAMNRKESTETGSERETNMFELQQLRAELAEAKAMLNGTRSSLQ *F FQEQQQPEQSKATVSSVIQTAQFTQAGATKENTTFHSPQRSNERAESQRFPVDALALAKE *F TITDYDGKTCARAWITVVKNIARTFNIDDNHLRILLITKLKGNAQVWLHAHPARLIEPID *F NLLDQLSLTFGEQSSKAEIRRKFESRKWKTEENFCSYYDEKMALSNGINIDDDELLDQMI *F EGIPLQNFRTQARIQCFSTPSEMLRAFSNIRLPARREPPVQPTDYKDAIRCANCNSRGHK *F ADICKKPKREPGSCYACGQLGHLVAQCPTRKSVSSNNYVRWFKINFFENAYKPIISECLI *F DSGSPISIIKKSLINETMKLALVNTCYFGLNNCILKTHGQTTCYVLKGSIKIYFRLIIVC *F DQSMRYNVILGRDFLTACNLNLDPYTLGMIALRKPMEINKIAMFTENDSPEKSLENEIVS *F PKSLENEIVSSQSLENEIVSPKSLENEIVSPKSFKNATISPKSLENKIVNQQHKETGPIS *F LRDEIVNQQKNVSKSKLSEDEIVNTSKEIVSFKLPKDKNVYEQLNHNFDKEVLRICHVTE *F SELEYKIGENVSNRLQLEFDRLFRNFYINAKRPNEPTVRSEIQLCLKNPKPFSCSPRRLS *F YTEKDRLQKLLDEYLENGFIRPSDSEYASPIVLVKKKTGDLRMCVDFRKLNKMTMKDNYP *F LPLIDDLLDRMNEKTVFTKLDLKNGFFHVHVKKESIKYTSFVTPLGQYEWLRMPFGLKNA *F PSVFQRFVNKIFADMIRENKVVVYMDDILLATENINEHLETLKEIFKRLVENKLELRIDK *F CEFMQSSIKYLGFIINKDGIMPNDKGIEAIKNFPIPNNVHTVQSFLGLCSYFRRFIKDFS *F RLAKPLHDILKKDKPFKFGSEEMICFNMLKDKLIQSPVLAIYNHKHETELHCDASSSGFG *F AVLMQKKEDQKWHPVSFFSKRTTDIESKYHSFELETLAIVYSLRRFRVYLHWRTFKIVTD *F CNSLILTLSKKELNPRIARWALEFQGYDFEIVHRAGSRMQHVDALSRCTNIMVIQTNSFE *F DNLVICQGKDTKLKEIRQLLENTENKLYEMRNGIVYKKTNENRLLFYVPIEMEEQVLYKY *F HNELGHVGRDKMIEAIMKNYWFPNLKQKCSTHISNCLKCISFSPKTGKTEGFLHNIPKGN *F KPFEIIHIDHYGPVDLARPKKHILVIVDAFTKFVRLYATKTTNTKEVIQSLNDYFRAYSR *F PKCIISDRGACFTSGDFDSFLKECYVKHIKIATGSPQANGQVERINRSLGPMISKLIEPD *F QGLHWDLVLEKVEYTLNNTLHRSIKQYPSIMLFGLQQKGQIMDELKEKIEEIGETIEERD *F LESIRNKGEASQKIAQAYNKEYVDKKRKRSGVFTKGDYVMVKNFDSTTGIAKKLIPKHKG *F PYVISKVLKNDRFLLEDVDGFQISRNPYRGVWSML *F >38C.29 *F MLGYRIQDTAPEGYWYAGADINMRRLLQRSAADKMASWLIAMASCRQSAVPWLPSLLEPR *F PPIQAIRQRRSLSASDLFALSPCGRGLMYVCVRRFNGGRPQVGFKMYKDVPFS *F >38C.30 *F MVASLVGFNLCGSHVARTQSHLQRLQHKYTVKLLRPKFRELARGIGSESVGMEGQIGNYN *F CSKAAEVTNSLTLAFTPSPGCHAMDDCNVNGSEIEGLVGNAGRKSGLIDFKIESNQINLQ *F APFVMEKYAKFQLGKNKLGKIDAAGLLCALLTLSVSLSISILPSALINLNFHRQMAGKSF *F QLLSFPTVLLLPRFPAEGGLHFHLLRFKGLSFKARLKCRANI *F >38C.31 *F MAEEDRICRTFSHWQPFKYVMALWVSWLDPLLLWVEEQTHHRKEDLAQNMRGSGWMWVEL *F GGIGGNLRQWVVVRWGWWAKRPLVSKLKAGQVPVSNINDGFGSN.MSISLIRKHLNEMGTL *F KLRSSMGRNYIPMAGPPRFPMSSAQRLIFGCGSLVVMMIIPFYCLFSLPRWSRMHLGLPP *F EEDEQAEEPPPQEEKDKEKK *F >38C.32 *F MCPLVPFCHHRPHSALKTCFIFVVMPRHSSGISGSHGAASLHFSWALQDQLFGGRNGDRG *F SGNHSADTCRTCHTRFVALRQFSVSEAARRKLPRCSAKSETILTEIFIIIKRERRSGSQI *F PVPIHRWFPIAMSLSSSNIAAHKEFALCPPTDSNSQIQLIANRLIHHIEPITYITPIVSA *F HTLVTISDHPNPQTQTRKKASQTQIYGVVNTPAPLGTTDRFVPMKISFRLCANKN *F >38C.33 *F MSTKQTFEHPAPVEQRDLPSIKEVIKVDPSAGPKPLTIQEYKARTAAREQPPKKKRGGRR *F IKLLSARRLNIELLKTATNEEDRQRYKERLAAINQQLRGAKDAFDKSYKCINKTALIKTQ *F TLIFHIKVLITQYNTLQNLIVTNKSKLTEEHKVQCFKVLSSFGKRLHNTSVRHSIIIEVP *F TELTKIAEFDESQLRDLDESQPLEDLDIESDIESIEELKFNTVQPNTRNMANALEAQRAY *F VKQVSATVPEFDGKKLHLNRFVTALKLTDLTKGDQETLAVEVIKTKIIGPLNYKVEHATT *F IQAIITILQANVKGESPDVIKAKLINAQQRGKTASQYVTEIDSMRKQLEAAYIDGGLDAD *F NADKFATKESISAMTKNCANEALKMILTAGTFSTFNDAMEKYLHCSTEITGNSNTVLFYN *F GNNRRGNYNAYYRGRGRNNYNHNYNQNYNQGYNNNNRGRGGYRGHGNNRDGGIGQGTIQS *F IGTVDLDIRIQDVLVPHEFHVVPENFPIPCDGIIGIDFIKKYNCVLEFQNNKDWFTIRPN *F NFSRQISVPITHNLDSNTLLLPARCEVIRQVKLLTNEKTVVVPNQELQPGIIVASTIADS *F KNALIRIINTNNKDAIIDSAKIKCESMKDYDIFTTPVEKENRTEEILKQLRFPKQFNNEL *F TKLCTEFSDIFGLETEPISANNFYKQKLRLGEKTPVYIKNYRMADSQKPEIARQVKKLID *F DGIVEPSMSEYNSPLLLVPKKPLPNSTEKRWRLAVDYRQINKKLLSDKFPLPRIEDILDQ *F LGRAKYFSCLDLMSGFHQIELEKRYRDITSFSTANGSYRFTRLPYGLKVAPNSFQRMMTL *F AFSGLEPSQAFLYMDDLVVIGCSEKHMLKNLTNVFELCRRHNLKLHPGKCSFFMKEVTYL *F GHKCTDKGILPDDTKYEVIEKYPIPTDADSARRFVAFCNYYRRFIKNFSDHSRHLTRLCK *F KNVQFEWTAECNDAFEYLKTELMKPTLLQYPDFGKEFCITTDASKQACGAVLTQDHNGQQ *F LPVAYASRMFTQGESNKSTTEQELTAIHWAINHFRPYIYGKHFMVKSDHRPLSYLFSMKN *F PSSKLTRMRLDLEEYDFTVEYLKGKDNHIADALSRITIKDLKTINREILKVTTRSKAKQE *F NSCKDEAIVKIQEEKEQTIEKPKVYEVVNNNDTKKYVLIKIDKHKCLLKRGKTIVSRFDV *F DDLYSNETFDLNQFFQRLISKAGMHKITKMRISPSEQMFQFVSLNEFKIKGNRVLEKVEL *F AILQKVIIIDKNDEAQIKEILTKFHDDPIEGGHTGISRTQSKIKRFYYWPQMTKTISKYV *F KTCLKCQQAKITTHTKTPLTLMPTPATAFDTVLIDTIGPLPKSEDGNEYAVTIICDLTKF *F LVTIPTPNKSAKTVAKAIFELFVLKYGPMKTFITDQDMKINLKIIGSGEGSGASAIGGYV *F RKWLLLSQPPTWRSVKQTDKTCQSVNPSDTDAFGHRESEGEGLALALALA *F >38C.34 *F MTEHQAEEQADTPPTKVKATPTPTPSGKFKDAKEDAFLSTSPDSANGDAQHKLPADQLAM *F SSSAHPQQAGQARPLILQAFHRCSSPEQCVTLHDILDSFKAPLSEDQAWALIHQFAGLYH *F QVAVQAHTCAADYEAALPTGFELHFHRDGSVHFSGPDQLTPKEQLQQEQIPLPPQHDVIV *F DQPDHSASSSGDSSVINRGKQTDLLKNQYAYIFLTATVSGN *F >38C.35 *F MCPLNGPHKCHPHNGGKARHNLDYRLIYWVCDALSKPKSLALSIYDCVRRRHKVFPFQNA *F ANGQRISFKRQFYRCARENVENSVETAARLIEAPEGAATRRRRRRFPIKSSVSHAPISMV *F AGAPEGSDGLRGSGAEFDTINDSLRPWMRLSGGDGARFSARDQRKSRPKSKSMRQVQSPH *F SNNVTCENVPPI *F >38C.36 *F MKYRLKTFAPRLRVLMYAWEPISPISPQFTQPIHQHRPSNASIKTLSAVPVLELFIVCLT *F VSDEEEVERPQASNIDVHSIQMLNVCLGARKIEQLVISRVVVVAGVREPEPKPENQTKHK *F ANGEALNQVAIQRQLIVFGAGQRRQQQQPKLQVEVAATVACCCRCCCYWSIRHTNVPGRT *F EPIKVRACQLNGTVCIGNCSSHGVDFTSKIFIPNRCPDVKNSLSGPIRMKLKSAPTLHCK *F VQLYENPFMAMQAQQMELAKPLAKLMEDIGWCGDSQIYRGGLAVSWRYRERYRERYWERD *F WDSHQGAVSSRVESESLDYSLVAPAQSSRPVENRHQGELPQPETPTPPSKCVKRNWIGG *F >38C.37 *F MSLNLQRLQLQLQLQLLRCNRHCSCNSSANDIISELAEIVYTALDYNLPEDEECQVSQEL *F ENLFNFMTADETDDDCIDEGIDEGDKRWDDESEEERNDTKELEHIIEKSKYLADTCCARI *F CIAANRKQADLVRVLVRYLAYDARWSDRLDFNNFNCGSIASILNNRNNHKTKRFRNHPLE *F AHTRIPDLGRVGRVMNLTPLLIEPVSSVPRFCLSAPSGAGGAGWFLSNELYGIDTLLSGN *F TEQNHIKTTLPENHYRAVCRALVTETIELRVFLQQVLNNEAGAEKLIKASESSATTQQEL *F AKLGFNDWGKKGFPRKSLFSADIVSVSRLAAFDDDDDLARFWVQVIDELRRGVRLKKSNH *F ERTPIEYELTPYEILMGDIRAKKYQLRKVMVNGDIPPRVKKDAHAMILEFIRSRPPLKKA *F SDRQLGPPRMCEPSPREQLMESIRKGKELKQITPPEAPTLRERAASVFRSHHMPRESDLV *F DIGSDASLYCGSDGESSQAQSTSTSTPNPQTSSDQDLDQPQPTPRAAPRASASNNPPTPK *F PRQAIRSSKVLPSANSTLSRSRQRLIKVDFSKFQDDDLFYDENSISSSHSTAATHQHHPH *F FAEMHRCSQPKMPPYPFGGYMVPSQARQDCRETASLMRPRRTTYDLATQCESRRASLRRH *F TIVGCQSNLDETHSMPPTRPESRQSDDVSKETPKRSPAEQTHPSDEGSSTSSLGPWNKSF *F MDKQTWMERGDDRLSVTLAEIVHIRSVMTKAELEGLPMDVRVKEDVEKRRVCFLCLRTRF *F SFFGPWGIQCKLCQRTVCAKCYTKVGTGLMVNHRAAYLLFSISSQMRIPSEHFRNVPLVL *F ISPSLLSSPASSSTPSPSHHAQQAHSSSTGNIMDDQFPKSLIERLLRSESDRKVGLLTAV *F RPVNMELMKLIPLFQTRSTVGSAPSSPKHQRSNMSTPGISVGPGASSSSAAATGQAVEAL *F HDQATMSSSYSAAMRPSGVHQQQKQHYNNAMSRSMEGPRSLPVHSPAYRPLSNNSTLERN *F HLAQTQEQKENLRGEQVTVCNDCQGLVNEITSSVKQKRSSARNRTIQNLTLDLTPVWK *F >38C.38 *F MAEVAETPSVEAQEEVAQPAEAQVLEAKNGDAKKDPAPAAEEAAGGFTKQERAIIRQVEY *F YFGDANLNRDKFLREQIGKNEDGWVPLSVLVTFKRLASLSTDLSEIVAALNKSEEGLVEI *F SEDKLSLRRHPERPIPEHNEERRKEIQERTAYAKGFPLDSQISELLDFAANYDKVVNLTM *F RKHYDKPTKSYKFKGSIFLTFETKDQAKAFLEQEKIVYKERELLRKWQVDYLKEKQEEYA *F QKNEKRKNKKEAKPEPAFELPKNAIVVFEGAPETSSREEIREAFEKIKDFEVAYIEFAKG *F ETKGSVRLTEADAAEKYIAKVEEGKLKFKDEVSLSLRKATEEEEKEFIDKAIEFMKKRRD *F FTRNKGKRFNRKRHGGNDHKHGGGKKARGD *F >38C.39 *F MGDIYFFDNKLWYDNLTFFAVFLLSDDAPHAFIASAKLPRKAENTEKLATYGRRIFHGNR *F KCHSRSHFLRVSSDRRRFEYIFTYTDTDTSFRYRYTGPTLSEYKRQLFRLLTIRRRRRNS *F NEYPCRFRKCAIWRVKRRIHRRGAPQIKSPNVYFRGTLGNVNNKDERCKRKASGLFCLAK *F SAAKTNKILRGGGSHRWLWAHRRGERVGVPIACGDPLFSTPTHQERVHFTFAASASKQTL *F FGRSWNGCPTRSPAIDPMDYAGGPLNSRNRQQITIARAAAS *F >38C.40 *F MDQPLVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECK *F VQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYA *F QLMCNFVEIVRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYK *F DELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVA *F VYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHRDRYFVL *F FPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGP *F NEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHYISPIYSPLLSFRFGDTLNR *F EQLSQHLDSRGYCTRFSLCAYYSSPPCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKG *F GGLRSAIVKMLLYPQARQSIDLKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIE *F LPTDSESYDDDFEDDFLHSCDSDPFEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSI *F NLIKLDSADTDEVLALNELKKESLVIGSRALRALARKSTTRNSSVHTSTATLELGVGGSI *F TNCVEEEPILKVKPSFSLQQQSSDASSIFAARLGGAFTACENLASMPDDLSRESSIQEPP *F TPLPASPTERHSMPTIFVGNRFNHSKNTEVYVPTWRDRQEMQNQSVDAKQDEELHSSSID *F LPAACLSAPDKLQAELLYNYDEILEKPLQLHRELTPFPGHNLNSDKRVSHKSDSPSTGNA *F KTDPNLATRSSSTTELCIDTTSKKRTTPSERSRDSIRRCISYQFLQMSNRPPPPPPPRRD *F PDLHLDTKCRCCENSQCPSPRSSDSGMAGSCTITSPDPPNPESYFPMEAAGHDMLDNVEP *F ERFDVCGMFREKFLTPEATQDVVDLSEEQPQLSDEPTTPTNRKEEPTCITSAQVQVNTRS *F IFLPSSSSMDETNRNVPANNILFSSSSADQLEPQATFRSGMYAHWWKKERLPPEVVRGIA *F HAYNKSLPSKDSKDSGSVCSSCFCSLGASGYSEGALYCSVCQNCADYYNGSVTSTTNTTT *F TTSSASCPLCSEDEGMIAPTHDSSSLDCPICNGRIASGAEEGKQSEMDRRPATIWPNAAR *F PKKSSHIASSSAHPLTNPVRVHYLPKFPMELLNELQNINLTLYNTKIRNENENYNQKPIK *F RIRFDLLAALNSLQDLPEGFTCCYVAAIKPHTPTARRPSASHSQQHPGRQQQRREPQPPG *F VSGHQLDVASQTEPVVQPPSQLQSVHAHSASTTSSASTTTTKSAKSSGGIRPQIKFSPDT *F KQQDSSSTPGVIHGRQSSNSGSGSSSGGSANWTISCLRPTPPLRPSLLNATSGSGSGSGG *F SGGGGSSSSNALASYCSGRKNQPTYEEDALVLRVFEAYCAAYQNNARNTIHSALQPWTPC *F LPIRGGKLKTSKTFSTSNPQLPFIANVGNSAYLNQHQQQHLQQQHLQQQHLQQQHLAQQQ *F PGRQHSAGSLNSSFIWREASPNNFNLYSSSVSSSLNATPAQRYSLSGTAGGSTSIKDYQR *F ALSEERATRKSLNRLKSGFGSGYDNVCTSPLLPRKNKPAPKLVAQQSYQRSPGNATATTI *F VKPNPPGHPGLYDRRLNRSFETSTSHRYAGYGAGYLMNCGGALRTSTLQRSTPQLAGGER *F SDDSDVERELLRGNGAGPTASLELNPDGSKRQSGSWCCGLENEDMTPTLRQLWTAIRQMQ *F QDMSQIKLQINEERALRADLQQLLMQHLETSSRHKLSRRQKNDPTERHRYWYSRLHRRTY *F TQRWLASILDGSIRSVSMQCDASNPIHGPLKLAGLIFKTVRFEWIVGQHCAGHKSAPCVH *F MKAIRSARMGQNARWNWPGQLISKVSASRTVMSSEPKWNGHSEEREMGMGIGIVPGFGFG *F NWLAMAMVVAENGPRWVLVNSDGAMDRWTDRLGMPPATRKTRRTEGRDLKMCLEMRDGQE *F RRGIYGHDKEETKLRVQRIATPRMLFWLGLSWKWAGYGYKVAAKVVAASCTLQVAVADVL *F VLALAVAAATDALQLRPCIKLTVNSARRGVRYKY *F >38C.41 *F MLLLCLLLLLPSVSPVRLPKSSSNNATAATTAATASTESTATAATTAAIRNANAKAGSKY *F AATSSIIIVVRSSSYSDLTTCPPWLIHVTLRARSTITRSWSGSAELVTPTGSTLQIFFCR *F SAKIRGVAGEPNYKSVNLTWEVEFVPSAHDTDSSPNSNSRADQVNATNMSGDVEPPRDLA *F FQIFYCEMQNYGPQRCRVKLVNGTTAEVSQEENEKATDQQEKHEPSGSQVHHFVAAVDNL *F RMATKYSFHVRPAAQKRLQAGGTRSSNARADFHDENNEIESGSGHLAGQSIVIPTKGCKW *F FPKIEVETGPYFGGRIVVDGGNCGIKGDASDAADKYTMRIDHKECGSLVKPETNTVETFI *F TVQENLGIFTHSTRRYHSGMQTVRASFTVPGKNGVAAAYEPNDPFEPDEDQRLGRELRPM *F RYVNKTELVLREPDSQRESQSDSESVEQAAVVEQAPTPTTEQASQPRGQGRALNLNEVNS *F LADEPAEEHHLEPVVGTKYAKLVVDQSHSSWMPLEVGSPSGGSDENEAVLRYIGSHLSSV *F LVTVSLSVIIISICIVLLQRQRIRSPPRSPSPCLAAHLPHKTLPRALQQQQYQCTFSPLG *F SPLQLLLRLKRKPANIKHETRQPTKDINCNRVGSGLSGYCCRLPNSVEGRLQLKSNTKFK *F PSPESKNPKSGHCRLGLATSFCPSVRMDVEFSGTIKVSYKSMIKMVGRTKSYFVIVLALT *F LLIYGAQGANILGLFPSLSPSHLIISMSVAKILAEQGHNVTVVTVLEPTITHKNINLVTV *F PMTKEEIKQRSETIGAKQKNTSSNRFISILNMSGQMDSMLRKMADVLKDQRVKELYLNKD *F NHFDLVISGYFMNDYQLGFARKVNAPVVVLAPSPPSQMLNSLIGNPHDKVEKEKGMTFGQ *F RLDSYISSLLYGIFLRQIDQRNRQYYNEIFGDDPTMPEYTDILRNTSLVFFCSHAASEGP *F IRPSVPAAIEIGGIQIKDKPDPLPKNLEKFLGNATHGAILLSLGSNVQGSHIKADTVKKI *F FSVLSNLKQRVIWKWDDLDKTPGKSDNILYSRWLPQDDILAHPSVKLFINHAGKGGISEA *F QYHGKPMLSLPVFGDQPGNAHAMVKSGFGLTLSLLTLEEEPFRAAVLEILSNPKYSQRVV *F KFSSLYRDRPASARESLIFWTEYVIRHHGAAHLQSPLVHMDFIAANNLDIYALIGAISIG *F LLLMLKKVVQITCRRLRTKLNKVKKQ *F >38C.42 *F MSAALVQKATETQLDQTPEVGVAQLATISLLLQYDIDNTLNALKEHKKMTLDARLGGEVA *F AAVNNNQDKDINRNLPEQTELEEDLRLKSGENGFMPPTPPATPNLQSTAQKRWKILAKVL *F RKDSEETVSSSSDEFAEEQTASVRRFKSFDLLRQDSFEDHVSLKCLGKTENWYKYRMQLE *F NESEYSVNIHHMERQLTASDLMGFNNTGNICVWPSEEALTALVLSEVASYRGKWILELGG *F GFTSLAGLMLAKYATPYAVHLTDGNEISVENVRKTVCLNELSCYTKCSVLKWQEKSARSQ *F AEQAKFDFILCADCLFFDEARSALVDTIWYYLAPEGSALIMAPRRGRTLSVFQDECVARG *F FRVELATRYNETIWQRHLQLKADSALYDEDLHYPLLLRLCKSHS *F >38C.43 *F MSEEKVFHLPLAVAGGKRRAVGWKTRKNSEVSGMTDLCFDKDQEQQQGCVWRGMGMGMGM *F GACGSVLARQPESQPEPGACLNAFIAQLERHARSTGRTHSLHEELSAVEWNPGNWPNFTY *F SIGQTTNIGLVAINLSIGKTYRYIQIYVLAKTKGLGGWGARKSQGETESPTCVLRHGGGG *F RQPHKGLVRQDRQDNGQLTCTDALTPLRSETWSAQVTKPSAPGIEPRKYTGKLQEIRISD *F GSQTQSPGSLDEPICASEGVMGDKGIGCVLDLWPRGDGYWLLCHKPCVRVCISATQLILI *F SVTLVPFADEWQDAGWAKTQGRALAAKVATNSMKFNETLVFRPANVAYERRRQTEAKIHV *F RKAKQLGRIEHVGVGWHDESHRTCCDCRKFAFIHKKEGEQACGRRKSVGKASNDERGKRK *F TIKRQAATRRRRRQTRQPGVLSENRGGAEKRGDVGGKSSAFGGSVLSIGSLLIRVGLASS *F KLKFQRLQSGDFTSESLLSFAYLAFSICVNVVGRCQCPEHGRTQSVEAPIPNPHAHAHAH *F AHIPTRAAEGKLPAHVKLMYKRSRLVCIRNNY *F >38C.44 *F MERNEQRSADTDAATARNKSHCQNSEVIANRQTRALSAKECESLLPRWQSPGNEHSNEAK *F TKVRAEPVPSGSFELQPLRFPPAHFSSRSRQDEGKFMRLAAPVSEMAPRFKAPSKGCGKG *F GRGHAVEQQHDCRKSKLSTISHRRNDRKAAVESSGSQRCRQSSSQQQSFGHKLRILYIIT *F AFRIKLIIKLIINNNNKQSYMGARPVLNRLYEEHCRCLLVLVPCRGMFTRTPPTNKKLNT *F DQIQAILENESEDESRKEKMNEEDQKLAPVGEAEAKKQNKDASAKVEEKFEQMMNTLTQS *F MLAKSKQEGQVIIAAEKFEKVVSDCDGKSIPIKKWFEIFEKNAEAYELSEKQKYVQARSK *F MIGSAELFLESECVSGYTELKELLIEEFSGSYNSAVIHKKLQDRKKKREETLHDYLLQMK *F KIAALGEVETVALITHIVNGLDIKKEYKGAMLRCKTLKELKQEFEIYESLNIVDKPNIQP *F KPKQITQGVKADHCFNCGSREHKRKDCTLPTKCFSCNQEGHISSKCPEKVNSMRIHVDSA *F RTKPVIINGIIINCLVDTGSDVTIIKEAIFKKMKDVDLNRTATVLRGLGNASTQPIGCFR *F ALIKTDQVEASHNVLVVHDSKFSCDGIVGHDFISKFRLICSAEGYTFLDLEADKKQAVEY *F SQMFNICEESSFTVAPQYREDVERMIERTYETPPKQIKQCPVELKIIPDGVIKPFRHGHT *F RLSEEEAIAVKKQVEEWVEQSIVRKSTSNVASRIVVVKKKDGTLRVCVDYRKLNTMVLMD *F CFPVPIMEEVLEKLQSAKWFTTMDLQNGFFHVAVEEASKPYTAFVTREGLFEFNKAPFGF *F KNSPAAFIRFVQFIFQELINSNIMQLYMDDIIVYAATPEECMEKTEMVLKRAAEFGLKIK *F WKKCNFMQRRIHFLGHIIEGGQICPGKEKTSAVNSFGTPQNVKAVQGFLGLTGFFRKFIP *F GYAQIARPLTDLLKKDAIFNIGPVEQQSVNKLKEILVNEPVLRIYSREAETELHTDASKD *F GLGAVLLQKFEGSFHPVCFWSRKTTKAESNRHSYYLEVKAAYLALKKFRHYLLGVPFKLV *F TDCVAFKQTTKKADVPREVGPWILYMQDFNFQPEHRAGERMRHVDFLSRHPQACMMITSE *F LTARIKKSQQNDDSIRAILEILKDRLFQPYKLKGGLLYSMVNGNELLVVPALMEREVIQS *F AHEVGHLSLQKTMHSIQQQFFIPHLEYKVKKLISNCIKCIIHSKKLGKQEGYLNCIDKGD *F APLHTLHIDHLGPMDSSAKQYKYILATVDAFSKFVWLFPTKSTGQEEVVKRLTDWSNIFG *F FPKRIVSDKGTAFTSGAFEQFMSSHNVEHVCTTTGVARGNGQIERVNRLILAIISKLSSD *F EPSKWYKYVPEVQKAINCHVHSSLKLSPFEVMFGTKMYTRVEDRLLELLQEEVVCQFNED *F RYEMRQLVKRNIEQAQKDYKRNYDKKRRAEYKYKAGDLVAIKRTQFVAGRKMASGYLGPY *F EVTGVKDNGRYDVKKAANVEGPNVTSTSCDNMKLWKYIAENADLLSSGSDDDDQEGRINM *F IVGNRSYRQSVIEGTIARQQWSRVEVSVAGSRVLSSSRSVTN *F >38C.45 *F MHLVRLKAAKRTTWVPPPMPMIELMAAKAWSDDMGFGLTRRMDGRCHSDGRTLFIHPLGP *F HHSTRGIRIPARTSNACRALRLFTQETSLCPRRTIRRHLAGARVYYLNNNFRVQAALFDA *F QLGIPARMRMGFGSVSLGPDQLHRANRRRQLSESRICWHSRFPRAKCTPSDLFIHLLRTR *F EFVRY *F >38C.46 *F MDDDELIKLIERHPILYDKDCARSVKNAAQKDAAWKAISQKLGASERACITRWKSIRDRF *F GKEFRRFQERPDEPTYWDMFPRLLFLKDHYKQGLARNESLDGMRFEPRERKKRTKVDMEQ *F ERRRKDEEEEDSQDDLLNEQLIELVKSHPVLYDRHKIRVSKNLAAKNEAWREISENLNVS *F EELCYNRWKKLRDRFGREFRSHQINQSTPITWRYFNDLLFLGRHFRKGVPLVLENIKRRG *F RPPKAGNPSGKTSKQPEGMVISSGEQIWGADYPYSTDNDDLEDDLELAYDEEIEILSEAE *F QATPYDFILSEATARQDLEPPQQLQVTTTTPATSEEIIHTIARVNPVVEESSSLPGDSVP *F SAAISDKLLTTVIANMETVLQQSRELQAQIHHEQEQEREQRSTQPANSLLAKAQMLLDGL *F SPSERASAERKIVQFLCQCQIKALDGEEIEDVAPCQVIN *F >38C.47 *F MILLEINNRIIEETLLVKYRNAQAGLKPESIDIRIADFDGVLYHISNVNGDKTKVRISIS *F LKFYKQLQEHGADELLKREYGSLLTDTEEGYNVSVLINLEEIPEDCEQIAKRIGLLKRNC *F FASVFEKYFDYQEQGEEGQKRAVINYRNDETLYVEAKPDRVTVVFSTIFRDEDDVIIGKV *F FMQELREGRRASHTAPQVLFSHREPPLELANTDARVGDNIGYVTFVLFPRHTNKETRDNT *F INLIHMFRDYLHYHIKCSKAYIHSRMRAKTSDFLKVLNRARPEPKNTEKKTIT *F >38C.48 *F MDDDSDIFDEMWLESVVIAGFRVWHIIAAVLGVLLLISEYIIAVASVWTQFNRIIAAVIM *F VCCCIRFRIPRTKQEIEADYQRKQITKKFREKLQQIKNSEMDDMDLQKVCARIQSDYLNF *F QVSMESMNAKQNVKFKI *F >38C.49 *F MPRDLDSGSSSKTDSITDSVSASGTNSGESGVGNSYIPGQQQQQCTYERFCFDRQTLHAY *F RRQIIDIRLVALRRGVASHAMRFPPPSLTGDPNEQHRAVASHTSPRDPIRCSVPTRTNAS *F NQSDSAVCSTSDVVGSIKFTLPTMFYCLPKCRHPKSLTLVVVFLCSSCGFHLRAPIFHAF *F SVFPDSNAFRLLRYNKLSRKKGRKAKSEIQSGVKTSAKVNNLWESSVRPRVLESCQCAQC *F FKCAKCASACAGEATTSSTKPSTSTAAAAAAAAAAAAGSGSGQGSGAGAVSGTAPGHQRS *F GGRSGQRRRSSSGNPHAGTTKATSGSGGAGSAGGGGGGGGGSGGGGVKQSVSRNSNIRSS *F SSNSTMASANHRGGGGRGGGGAVAGAGADGNAIVGLQIGSAWFQRKINLRPQHRGVHLVT *F EEILRQMPELAHFSVGLCHMQRCLHTSDAKRFESLFKFYIPLLGMHSQ *F >38C.50 *F MAPTERAARSTTRGKGLARVSFAGEQRQINQQPAKAKPLGGVGLLELELGVLGAFSMADI *F FLCGRARSFLFALS *F >38C.51 *F MDTHVQVPIFKGARPWREIGKRNTQPTFFLNNFRGDYVMLNMCKWHQEFKHYPQTKFSDN *F ANENENETKLTSDAGRAQQICKLIAGWDLIGNEDRNGTRTRTSTMAMARSESRPPHLVGL *F KTSID *F >38C.52 *F MEMMLNKIVPEGLPYRHSCEGPDDMLPDNPDHRRQAVAGNVAGRLAVRAPRPGRIPEAGD *F HADRMSARTGPQPAVTRLADRQHKNLAKANLAQQRAAAVIAAGRGGLGGGHQDALAPPNI *F PGVETKTGRLLLRQQLHLNLNLNVQETLRDGVDIDDVDEDVDEDEDLDLDLETVTTVPLN *F NEQLNAKVTTTTSSSSAASSALAKFNRIIDSTLERPPVAVDYSGLPQLSSATLQQRFNLA *F YPELVNQQQQQQQQLQLQQHHQQHDATKQISSLQGNPRLDRGTDAVASTPLTLQAPRTPA *F TATEEQLDSGHPHDDADVVDHDDHPHHDEDNQTKRNDVHYLLKQLNSFSDIEEIEIVDMK *F QRGQRPPMASSSLATMMPPPSPAPPASPSTQSHRLGPRFASSGDSSLVLPQHQFDDYLFE *F SCHYLETNYFAATYRTAMVESSSHEFRPSTNSSSNSFELHEMEPPSVICKAGVPPPLVSS *F HPGHPGHPDHPPPRYQFEYQAETRLTTSGVQTELTVESLAKMSNCSGSSSSSSATRAPPF *F FRCCYTPIDRWRERRQQQKSSSASSSAAQPPKNV *F >38C.53 *F MRLKSGVLSLLLLTTFLALGECFWPFSKWHGRNDNPSAPPEPKLSPVIFGHPASQYRYCI *F AVPGDGGSQMDARLNKPNSPYLICQKTHDWYNLWLDLEQLVIPMVYCWIDNVKLYYDKVT *F RTTHNTPGVETRIPGWGNPEVVEWIDPTKNSAGAYFKDIANELVKLGYIRKQNIHGAPYD *F FRKAPNENQQFFIDLKQLVEDSYEANNQSAVTFISHSMGSLMTLVFLQEQTLQWKAKYVK *F RMISLAGVWAGSFKAVKVFAMGDDLDSFALSAKILKAEQITHPSTAWLLPSPLFWKPSEV *F LAMTPSRNYTMAQLEEFFKDLDYMTGWEMRKDTIRYNRNFDPPNVELHCLYGEGIDTVER *F LQYKKSDISGETPKLIMGLGDGTVNQRSLRACKYWAGYSKAPINTLALQNVDHLHILSNP *F DVLKYIRTVMQQP *F >38C.54 *F MAMQKFFSVLLPDVKRELRRGIIECSKRGLLHSTKWLAEMHHGLADVHIDNEAPDEDRTF *F SECQLEGIAPEEYSDYFLAKSYYDVREYDRAAHAVRNCESSVPRFLHFYSSYMAREKRRL *F DSTTDQANLHEPNQMRDLADLLATLRMEYGKSRLDGYGIYLYGVVLKALNLNQAAEQMLV *F QAIRLVPMLWSAYLELSPLIMEKKKLLSLQLGGHWMRHFFMAHTYLELYLNDDGLKIYED *F LQASGFSKSIYLIAQMALVYHNKRDVDKAIELYQALLESDPYRLDNVDTYSNLLFVKEMK *F TEMAQLAHKAVSINKYRPETCCVIGNYYSIRCDHQVAISYFQRALKLNPKYLAAWTLMGH *F EFMELKNTNAAIQSYRKAVEVNKRDYRAWYGLGQAYEIIKMHYYSLYYFKIAHQLRPYDS *F RMLVALGETYEKLDKCENAVKCYWKAIDVGDIEGIAMYKLANLHEKLGDHETAVHCYIMY *F CEDERAATDKQSLYQGFITLANYYEKKGEYERAAYYAYKCLDSEDRKMEAKALLKTIDWK *F RNAEGQKKVKTSTAVANADTSSEDEMEWELQDVRVRVPITSIATTTTSTTTVAAESASSS *F SLISLRPDRNLIQGMRRSQSSRTSLTAPASGTTTTPSATAVTMSTEETPGTSGGPSNPPE *F QAPSDDNSSMEISSVSID *F >38C.55 *F MGEATVRAALLAVLGILLIAGCDCSQRFKYEIKEFQVPLDHFSFLINATFNIRYLYNDSF *F VDKSNARTPIFFYTGNEGDIELFAQNTGFLWEQAERQRALVIFAEHRYYGKSLPFGSSTF *F NTSLPEHLAYFTVEQTLEDYAMLITFLRNDRQMPVVAFGGSYGGMLAAWFRMKYPHLVNG *F ALAASAPVLQFPGITDCDIFYRIVTSVFQNAYNENCTLNIAKSWKLFETLGASEAGKKQI *F SDAFHLCNALKNDDDLKKFLDYVEEVYSNLAMVNYPYNSSFLAPLPAYPVRQVCYYLKEL *F HSTDADLLHAMSSALAVYTNYTQSAKCLDISVNSNADDSGWNIQSCNQMVMPICSNGSET *F MFRTSSWNFKDYAEKCYKNYRLTPKPYDIILRYGGRNLEAATNIIFSNGLLDPWSGGGVL *F QAPNDKVFVIILPEGAHHLDLRHSDPADPPSVRDARDKEAAIIARWIQDF *F >38C.56 *F MWWVVRAQLSKRAQRKRKQSSSIRNVSQCNEVTPPVPLVSHEISITIIIAGLVARLFSIL *F NWQQSLQSLLRSHIAVFESSAFIYIYTPFSYHKSSYAILQGLIDSRIRERLCGRVLRFVV *F EETKVNDQGNYGENTDRGTGEDAQALEAQFATALFDYEKCSKHMLQMTAERAKKVLPGPL *F ILFAVHQRSTTTRITYLAHMCSTRSVCRLPTQRCRERKRRSTRDRLVIFLNDHIAAAAES *F SASSTPSASSAPLCMGIKCPGAVAVALAVAVALAQHRTK *F >38D.1 *F MPVGEGIGITCGSSPAHHQSYGFVRLRLRSTWNGSSEQRQRTESILGQSYTRLYGHIYGG *F KTRRRDKAINMYIRKVPQSDPIRSHPIPSHTIPSHPIRSDPCRDEPIGTSPANSCRIVYI *F YTKLNNPQSQEYPVWCSEKILDNSLKVLENERKLTPSLCAVVILLKYRPKRCSKAIENFQ *F FLREGGLPVIAIYGEHRNNAINISDPDRRRAEDFQIAPWRNRAVHLRRPGKRLQSAGREL *F QIASMCQATMRPCDHVPARRLAAHILPIEGTTQRGYCRSGCFKHLYKRVFTLQPEHSRRL *F QFAPSTPRSIDRIVSDRIELGFCSGATSPYLSDECGDEKSLSPGFLPDL *F >38D.2 *F MQPQKTVANVLQLAPFSLQLPSVVVIIQQIIWFCLFRTTIIIIIIIIFGHAEPVRIAVKH *F PGSRLEHSKPSVQEHVDPCAPKWIYQLEIIAEHVAPSSGGGRHLGGYNLMQLICKIVTLP *F LTLHQPSYLSTKRQQLRDGKTTCTACAEWCICHPLAALFLVSFHIFHFPFPGPLSLSSAE *F RIKSKGKVR *F >38D.3 *F MHNANAGNAARSRIGILASFVLVEVPACPRNNDCGHNAHNVLIITAHSPRSSWGSLNVLS *F GPSKQLPGRRTTKDAGHRTQDQRVDTAWPVQFAFNVRIVLLILSVLIKTHTTFLELIFAL *F EKVHLQAVETK *F >38D.4 *F MSTMDNFFYKVQRKHPTILDDLRAVFKNSQSDSPQRSITLSQIRGKNSVFSSRGDNFQLQ *F LKWQTLRLFALRLPIVIWTMAAIKRRQQTAPGKSENLSANRGTSDSVLLSALRLHIKSNV *F FAAAAL *F >38D.5 *F MSNGDEVIEDDEDDEDDDDDEDENENEDEDHDDDNKDIVVLRTATARCHRKWPATSVSGR *F RTANLEECGNKRPQKLNDIRSGCGCEGNAFVRRAQIKDYAINTFI *F >38D.6 *F MASIRIRIRFIRQHLAARSVYECIRGWRRVLCLINPLTAFDIGTFWRSGHVDTFDTWPAD *F PTAPAAVAISLHDVHAASAEKRRELPKLQIKLKIQRAVCQNQHRVEACSSGHLYSCTVVS *F WPWILVYTRA *F >38D.7 *F MCTASMAEQKRADKFICNHTHTHIHRQWHTKAMRMYSVYKQIHLQAKAHIKHSGSRTGLH *F LTIYLQLSKLKPHTAPQLGELQSHNHCDGHGHGCAEGLARGMPVQRTTTSQYPWQSVASI *F RPGHRGHSKKLMRK *F >38D.8 *F MHRERRRQIHSGAVIGQRIAFALNRSRSHDRTTHSKPPRATHKQQQRTKSHRTTPSHTPT *F HTHTHTHRKFSHLQAEF *F >38D.9 *F MRLLSALALVRAITFRFDFDLLWHRQRHRRFYFIFICALPAETKFKLFFPLASAGKQILN *F KVERDKRYLLQSDCNESALRCKTQRQALKSNTIASTMQQQEQSVLVEYSGDAMAEGGFRQ *F KDPTMVTVATSGCRWMSCTCAPSTRKHRCQFLALACWGISAAIVCAVSETKEPLPYLHVS *F IVYPGTARKWRLDFYRFNQAATAAACEVGRRHRPMPREYPLFVVVQLPAGYLLKKSGVSE *F LIKVHQAGSPLNTVLIEASGKDLCSARWVKGSDVGMGNPIQPQNQARRLVSKPHTHTHSV *F AHFEPVLIHIRVIIHADCMNISAFT *F >38D.10 *F MALASSSGFLLQSSAPEFLPARNLKSCGNPGWAAGRLCSRGPGSLLHRLLVAFGKRCQHL *F RGQKWNGPQTERVKDGGISTHMAQRPEVVRVCAPAEKRNCKQSRAADLASFAWLWAPLSP *F FAPEAPTVDFSHGTHFTLGMQNARLIQCKMQNAGKPTHNKETDAGQMSYVDNSEAVSLTS *F GFDVCLAHHNRTGGAIENRIGLNSARLLTTRLRNMSLLPLWAIDLDVDVDVDVDKDMHVE *F DQGKRKGFCNFYVQFFT *F >38D.11 *F MNATHWRYCNPDHQPPRYFSPIREPSPWLQLKLELLPAALNTVSHAPSSFRSLPFPSVPF *F PFPFGSGRVGLDRVEGVLNRGPSTYIVSDPAGAAALAKAKRRGATIMQLAATVPKLLAYL *F LPLLHRSIQYV *F >38D.12 *F MMLNNAHCGDVDDDEDDDEDDDDGDEGTSTVYKHNKKIPLHFPFHRDTDKLRIYGDISRQ *F PGEFSKDGAKRTRLHSQKQLKLLKPQLDEDGSKGFVIELESRRRRKETIWGVPKWAAADV *F FGLSVGLFFSADSDSLLGCGSGPKSRIRARNLSRMSGQRRCQHHRQCNHHLAHSRSKASH *F LGTGLLRNWGNHPYRAQARMPSPHPYTDIPATFARNNKKPPKAGSRMALIFYCYYPVYPM *F FTHTHKHTNRSAHRRGGAHVRLCDGVRISCGSQMRVVPRFLPSHCYFTIHFPNGENNYEC *F RARESRALERFLGGQQLPEWSRWSGCEYGARISREKITPPEEGTESLTDGLVRPTDCQPE *F AATKSARCAGGCASGFTCQAVNLTHQEPSSHPASYPDIPVSGWRRTPGAIKNFARKPSQV *F NMLGGEVWRRGFLLGFRTLGKKFKRMWLEMVSRCISKVSKLLQNKQSNSRQHQRQRQPPE *F QFVPRVSRWLVGECAKYISATTGTATDADDATRTIKSTPFAVAHLLLNN *F >38D.13 *F MSFEMSEYKKPYINLCPMPDVPFFVLARLRLRLRSRCLRLGAKTLGAPLQLSPTFLSAFS *F PVSRIFS *F >38D.14 *F MLTLIGVCARRVVVAVSAVMVVLVSASIPNFVAFFIVCHFFALYLQQYRGKNFAGEKQLK *F QAAEKLLKNFDNLHKTFTSRVTLAASMFGDNSQRQRSSSVAAAPFEATTKPILRSNCGSG *F SAASANGQKQSCNPAPVCVCGYNMWSKLRFVRGQELVYATSGSPKDSIAIRQSLGFVLNT *F QALYRERLGKTHRNSFTSHHSTGCFDSIAFHRLLSRRSSAYIPTVKCCNARAILQRATSE *F NADARRKLHASWKAENARLVSALSLSLSAAARAARSFSRARSAEILLMLLMCCCCCCRCY *F GSAATASLLLLRCCCCEGLQCFWGCAVGPWAIARSVVVACCRWPGLLSSVWTAPPPTTTA *F AAAAAAAAAASACNLQHSHHGNRSRRVAFSPRPTA *F >38D.15 *F MSVQFAEGVDGKRFSLASSAFVYCRPFVWAIYFPGLIEVRVPADGEPQPKGRYDTGGIQT *F HSSGKPNLVGFYFQQPVKHSARMILIFFKCEYVKRQRGQCDVFIAFAPTVSGIFLLDCLQ *F VLCPVSCVLAILQLSANYRARLTQKQQTPRATKTGSNECGASSKHPYIISVPYTVRVYVC *F GPHAVTGRTMDVPPLLVYALATY *F >38D.16 *F MRATLPKNQVGSGGFPLRVLIALDGERWTKKSCISVAGACGNRSPTMPNKTQHNLCQKPV *F QIELNQAESMCEWARTTTRTRQQGQTETGNGRCVLKFVSACLLLKFNATLFVDLQQEKRI *F VKSCVECRAVWDVPGLARTKNQINRPDQKEMKALIKKEIESGQNLAPKINKLKLTAELDF *F LKENLQQRKPNTTTHSAKIEIINEEITENSTSPKPKRPDVCMNDCPRPLDLPSIKEVIEV *F DPSAGPKPLTIQEYKARTAAREQPPKKKRGGRRIKLLSARRLNIELLKTATNEEDRQRYK *F ERLAAINQQLRGAKDAFDKSYKCINKTALIKTQTLIFHIKVLITQYNTLQNLIVTNQSKL *F TEEHKVQCFKVLSSFGKRLHNTSVRHSIIIEVPTELTKIAEFDESQLRDLDESQPLEDLD *F IESDIESIEELKFNTVQPNTRNMANALEAQRAYVKQVSATVPEFDGKKLHLNRFVTALKL *F TDLTKGDQETLAVEVIKTKIIGPLNYKVEHATTIQAIITILQANVKGESPDVIKAKLINA *F QQRGKTASQYVTEIDSMRKQLEAAYIDGGLDADNADKFATKESISAMTKNCANEALKMIL *F TAGTFSTFNDAMEKYLHCSTEITGNSNTVLFYNGNNRRGNYNAYYRGRGRNNYNHNYNQN *F YNQGYNNNNRGRGGYRGHGNNRDRGIGQGTIQSIGTVDLDIRIQDVLVPHEFHVVPENFP *F IPCDGIIGIDFIKKYNCVLEFQNNKDWFTIRPNNFSRQISVPITHNLDSNTLLLPARCEV *F IRQVKLLTNEKTVVVPNQELQPGIIVASTIADSKNALIRIINTNNKDAIIDSAKIKCESM *F KDYDIFTTPVEKENRTEEILKQLRFPKQFNNELTKLCTEFSDIFGLETEPISANNFYKQK *F LRLGEKTPVYIKNYRMADSQKPEIARQVKKLIDDGIVEPSMSEYNSPLLLVPKKPLPNST *F EKRWRLAVDYRQINKKLLSDKFPLPRIEDILDQLGRAKYFSCLDLMSGFHQIELEKRYRD *F ITSFSTANGSYRFTRLPYGLKVAPNSFQRMMTLAFSGLEPSQAFLYMDDLVVIGCSEKHM *F LKNLTNVFELCRRHNLKLHPGKCSFFMKEVTYLGHKCTDKGILPDDTKYEVIEKYPIPTD *F ADSARRFVAFCNYYRRFIKNFSDHSRHLTRLCKKNVQFEWTAECNDAFEYLKTELMKPTL *F LQYPDFGKEFCITTDASKQACGAVLTQDHNGQQLPVAYASRMFTQGESNKSTTEQELTAI *F HWAINHFRPYIYGKHFMVKSDHRPLSYLFSMKNPSSKLTRMRLDLEEYDFTVEYLKGKDN *F HIADALSRITIKDLKTINREILKVTTRSKAKQENSCKDEAIVKIQEEKEQTIEKPKVYEV *F VNNNDTKKYVLIKIDKHKCLLKRGKTIVSRFDVDDLYSNETFDLNQFFQRLISKAGMHKI *F TKMRISPSEQMFQFVSLNEFKIKGNRVLEKVELAILQKVIIIDKNDEAQIKEILTKFHDD *F PIEGGHTGISRTQSKIKRFYYWPQMTKTISKYVKTCLKCQQAKITTHTKTPLTLMPTPAT *F AFDTVLIDTIGPLPKSEDGNEYAVTIICDLTKFLVTIPTPNKSAKTVAKAIFELFVLKYG *F PMKTFITDQGTEYKNSLMNELCKYMHIENLTSSAHHHQTLGTIERSHRTFNEYIRSYISV *F NKSDWDIWLPYFTYCFNTTPSIVHDYCPYELVFGRLPRQFKDFSKINKIDPIYNLDDYSK *F ELKCRLELSYNRARRMLEKAKADRKLRYDRNTNNFELKIGDKVLLRKETGHKLDKRYEGP *F YDVVDIGINDNITIKTGSKKQQIVHKDRLKKHK *F >38D.17 *F MRPLLSSAGLGQRRVKESMGMVVEVEVGMSLLLFDPLRCANKSAAAFNFCCPVVWQADVR *F ALCWHIRLHYETNTRTHKKAREEPIKQMKIALSVYFRKGCGQYTQRYYVYVMYGRDIWPS *F CPRLTDAAIDGAPCRQSAVSANLRHELSYRNDALHPISDSCSLLSAADATRLCRCDAMPF *F LSSFSIDVSADVVFLMRGNDAGAEWEWQWEWSGNWLRWVFKPCAIIDVSAGLDPLAVFGA *F ASAIGTTFAQDLLAAPRSPMAKCEPHFRKVGELSYDGTRRRMGCSGNREHHCAWHFRIQC *F GIANATDKTRMAKEWAVGGLTRLCHYKLKDISSRVLPAPKDLSRDDYFRFVPQLIEILTS *F AWDEGRRLNYETFDICCKSNTKLGAAVRPPFYFFLSLRVAETVLPA *F >38D.18 *F MANGQSDRQARNPASRIRRRPRPDLDFIASRNDTPSVRPAKPLKIGGASRLGPPCSKSFQ *F HDAQQEQEEEQPEQEQEQEGCTLLVAVPGSHRTINTVACSRMSVSARPWAPLLAHVPIIV *F IRALLPFALCPLCRVLGQKDPAAVAACCFCIFYDATQVAQIMHKPMKHATGTFANASRSR *F TTRPFSPAIFNFVRFSFLYYYFLLFVCRTLMKNFASGKCKQ *F >38D.19 *F MYLQVLLILVRTQVIPASAQLSFEQIFDLKSQSDAEFQSPKDSERLDRRLAQAFKPNLRV *F GRALKGGQSGSQAGRQAAQNFANLIKAKRLSGSGQVATPFTLNFGVLYGRKFQRTMLQSR *F GKSGREEWIFQENRATEWPPLAIEALMRDLTTELLSGPLCESYANNQDLKSYGIGGWEIW *F GWEGFATAFGIVRINKLANRTGGINFNLKISASINVLVRDEANKICQQKTRMTATDFKVN *F ENRKKARWGTSQNVEKSGQANKKVREGKGRRRRDRRKRQETRGDKQKWRHRWPLSKQRLA *F IEANDSVAGKDILPSPICTDKLQLPQTKFAARRGNSRVHEENSRVDGLLKPFEAIAASGS *F GKQVFRQRHRQNSKRPKTLCGFPSCSFFFFHCGFTAAVLWPAHNFAVSTSFPLRLCLFMI *F YVVSPWSYKIPGLQARKEENMEEKTTCEFCVGVESVEQVKQAELEVRDLDPWTMPEGYAL *F SRERRARQSTNQATNQAACYMQLKRLRDPDEHRIPPPQPKKPPSATTVRKTKLTRLENPL *F VAPTYQQQGNGCGLAKEKVPKMNAKRFTRPSKTQAAEPAPKDTIHDNAKICNHFDLCRAS *F KNPIRAAGGGNTQVVSKYQSAVSVSNQPVRSTVPRATHPKLAPKEVREARELIFKDLKEA *F RAAKEPKDFRHVKETKDQQDPKEPKEPKVPKEPKDLKASGARQNINRTKNYFDKYLKFAF *F DLSTPDGIQKLEDHFFPNQDPTAPGVSNSNREK *F >38D.20 *F MRQNIYEPLTSTAGKGEKRGERIICIAARWNACIAMLENDNQGSKNAENE *F >38D.21 *F MHRSGSGFSPGGPTGRSFGSSVGGSTKRIRRRMGSDGDGTGWARDGTGWTRNGHGNGRML *F SSRGTESSPCFRIRTNVSVKLTN *F >38D.22 *F MQALGPQQQRRQLAKSNQQKENASFSRGQCASPLTLTHTRAYHSEELSLYNATVNNRLTQ *F MRCINQMNRIAAHNNNACTHRNIQPTWRMRNCAPGLKSILPVQSSFALAAIAEQLQQSRM *F NSRCVECTSIGVTRKTSHDPQDHSIENIEKQQMPKTYLAKWRNNNNDNNSYLCDFVAAAA *F AICKDNKINVLQGLADSAVAYASRTFAQMFCDNKYYSVPHHRDRDRSSVLRPWSLVLVHV *F LLWSFAPRSLAFGPMFRDRMRSLWTGRHFEKCCTYFCIMSARKCQPTNDDNGNDNDDVDV *F PRFGVMTERIVADRGVAAERSI *F >38D.23 *F MKIIALLVLANLGFALSSKEYDEHERLVTWRLRNIVNKYKYLATGNAEFSRWIEKVNNAA *F ARSNLEVKLDTEGYFKVYDEQRQLLEDNITQRLNTLRSLISLRKGGKRCVRFYQHQENEL *F KNAYKFSNQKKEEACYPRRCGNAERILRYLASPTRRRLAQTGLLTAPEKQRSGTTINPCD *F IGQRVWVGLSEKKPKTAAPHKTREATRGAFMVPPICLIDGNGKLLVCKLQTGVRWCGEGY *F LRRRRPLTVPVWFQESAIHGGCCQKSGCSSGLRFRMHQMMWDRILDALTALVVPAYVVRI *F VRTYFSERVLL *F >38D.24 *F MDRLATWRLHNISNKYKYLSTGNPEFYQWIERVNTVADEFNHYNKHRQRLEDQITERLIT *F IRSIIAIRNASKRCLRFYLNQEAELESAYKSSNERKQQVLYQNSLAFRTANMKFMAICLL *F ANIGYSKYIVHLYRYPKTDTHLSNIYAVLGTTIGQLNDEATIRLKGLVEKYKLQALSNPE *F FSQWIGKLEKTSKSRRLEDKMKVKAEFKNYDERRLQLENKIRERITAIDDLISDIMAKVP *F IKDKGCLKYYQRQKRSLKLAHNFSNLTKQTNLIRNSKQCETTKVQSSELSESSENPDEYS *F YY *F >38D.25 *F MDSQRSTLVHWFRKGLRLHDNPALSHIFTAANAAPGKYFVRPIFILDPGILDWMQVGANR *F WRFLQQTLEDLDNQLRKLNSRLFVVRGKPAEVFPRIFKSWRVEMLTFETDIEPYSVTRDA *F AVQKLAKAEGVRVETHCSHTIYNPELVIAKNLGKAPITYQKFLGIVEQLKVPKVLGVPEK *F LKNMPTPPKDEVEQKDSAAYDCPTMKQLVKRPEELGPNKFPGGKNIVKLLITTTTMWISF *F YQTGETEALRRMEESLKDEIWVARFEKPNTAPNSLEPSTTVLSPYLKFGCLSARLFNQKL *F KEIIKRQPKHSQPPVSLIGQLMWREFYYTVAAAEPNFDRMLGNVYCMQIPWQEHPDHLEA *F WTHGRTGYPFIDAIMRQLRQEGWIHHLARHAVACFLTRGDLWISWEEGQRVFEQLLLDQD *F WALNAGNWMWLSASAFFHQYFRVYSPVAFGKKTDPQGHYIRKYVPELSKYPAGCIYEPWK *F ASLVDQRAYGCVLGTDYPHRIVKHEVVHKENIKRMGAAYKVNREVRTGKEEESSFEEKSE *F TSTSGKRKLVSALETPTKTMFRNLSMLRNQLALHRVRVARSTLHSKMSTGLTCLTIDRPR *F NIDGVTVIDININEMAKTDVEFNRNFVNSLTLMDHTPFKEVTNIVDADAVESPPAENPIS *F GDTVEILPTGAPSSVVGVDGNPIVPIEIDGWNQDEEDPTVQKNVQDVDIGNDDEYKAEME *F LRVPEVREGRTEYKGIKVTLPESANQDVGTYRFRRDPKDLEEVGDDTRLVRYDK *F >38D.26 *F MALQAHIRQLLNIKRGKHGGKMDLLDSILNAMDAPPANNEQQKTLIKKQREMLERMQNKQ *F KEELLRFRKYVDERMGRFAKDDRRCIEFQPLDKVHRSVIHEVAENGGFIAMSFGREDVDR *F HSVVYKKEHAPGEDEVTARRNGDGWNEEIAKEYAERRRERLAQEQSDKEASTSEAASSGS *F STSTSADQDSGEVKPTTNYKAKYAHLIGESAALQAARKTETNQSYGFVPSKNKKDVRSIE *F QTLADIQAKKRLRLAQQQELEQEQQAQEATTSTEDP *F >38D.27 *F MRFRSLKNIWPKEKLEQFLFLFILCGLPAIYYVLMEIILPELSDYWSPGYVFQLLLGLFL *F FSNVMSNYVMCILVDPSIDPKLMKNQLVRGQHSEDWHECDKCGILAPPRSRHCRKCGVCV *F LMRDHHCFFTGCCIGHENYRYFFYFLIYFFLSCMISLTSSSIFIYVLHGGRYQLFMLTHP *F APNSAYFNSLIIRIIYFKLPDIYELVFTLVFVLLWIGVCVATYVAYDQWSRGYFCYDFEL *F QNIPFDRKLRRNFKTFLGRRMKWTWISGFVPSQLDHDGFDLDPDNERSGEYIKVVESQCE *F TDGFVNEQWTEPAMPGPVPWKTIIIILLLFIGGIVCIAFATLNWVTDTSRERSDRVWALG *F IIGALTFIPGSYYVYVLFCIMLNRNGFTMDEIRRLG *F >38D.28 *F MRAAVGLMLSHGAGGMEPALSRPDYEQSALHHSCLLVLLRGVGPSRARVLQRAFEKVRRV *F NHIRVNGVCCSSSLAIFLITNNSICLTDSSGHPRSIWIRFVHDHPVEHNDWGDFQTHRRL *F LGLVTIGKFDSQIELNELCRQHESLKVRYGSTLYESRAIFFGPDEQPLETIGEVLGPPAA *F GGRRLQDEFTTPSNFKAQAFFYREQDSCADLESRIGDFASALFWVLESRRLERSREKADK *F VSLLLAPFEKRDFVGLDMESRNNRKRCVGRVMKNLADLSLQAGLVDDALSLYHNANETLR *F SVGDSLWVGATEEGLCAASAMLLYPQMRETETLHRNSSLQEAGTSPLKNTPEKWRASDAT *F KKISASDATANNVDSNQPQQRVTSNSSSCSSVSSLVTTATNSSASDTPTTSSSSTSTISA *F APIPGHQRNGDLPGNILKAEEISNYYRKAIINYSKYRHAATIETEAALKASRICIEQNRP *F LDVAMFLQNILYINLSMSEAERVKRFEVITDLYQQIGYQRKAAFFQRLAALKHVQQGSQA *F PDWNQSYRLMLGSFTGYRLCLDPLEVIENAAGWPALQIDLVQTLITAARRLGHSALATRH *F MTFLLQTQWDNMSPTEQSEMAVQLQNLSAQCEGSPVPLVLENGTVIPPANLTDLPYCIDL *F QVKDLPAHLRPQRIKVAKADSGPFLFTPIHFNSVDRRDKKKDKNKIAFQWVQNDLSEVTV *F RLRNPLPFELPVTDMRLLTNGVVFESLPQTLVLQPHVPTYVALHGTPIETGQLDLQGYST *F HTLGVKSNCRLKHMRGRSFPPNYVVDVIPALPRISVKTSLPQTATFSNMNSADIVVTSAS *F LTLYNGESSSCTITITNESATLPLEHLEFSINSNVEQELQQKIFRIDEEAIKAHLPVPPQ *F GTIEIIVDVFAEADFVCPQPPASLHSAAAPGDYGASSLTHYSSVSTSGHASLPSRVGSPH *F HRRNEPQNSSFRSTISGGPPSLAALTLHPGGGGGVGPSSLGSQYNQHIEAQVRFKYSGGD *F ALTAGYCRQCAVSFNLELLPSVQITSWDVLPAEVASQFYLVLDISNLTAQEMSLNYTDTK *F NILIEAKESCRVPIPVDRCSLEKVVAARAAEVAENLERELCFRTQLLSFNDALSKLCSIH *F IAERVKIKWLLTGTDIQGIASLRGIVLSQSMVDLTAVSPLEWAISFQDTLVQPHNEIVCT *F VGQRSLLSIQLANQSLQPLRNLVLSIKFYQDYLNGMENYNLETRVAISGPNRIAIPLLEK *F QEQKEHTCSVIFFTPGRFKASIECTSNPQKQSEQPSSLLTRSCPAEAESVGQSVMFSSSY *F DEQQAHVWKFIPPIEVTVVEQ *F >38D.29 *F MNLLPKTREEFAQTDYWNEFFKKRGEKAFEWYGEYLELCDQIHKYIKPADRILMLGCGNS *F KLSMDMYDTGFRDITNIDISPIAVKKMLELNAKSRPEMKFLQMDATAMTFPDESFSVSLD *F KGTLDALFADDEPETRAVVENYFKEILRTMRNGGRYVGISLLQEHILNFLLDFLPKHNCM *F LRIVHCLGVEQANKEKNADDALTLPVFVVVATKFKSLPMPVLEFGFGNDKMQRFTTVSEL *F NSAVSSVQKAALVCNGLARSNIAGHNEVIMDLHRPSEQTPRYTIHILDKPPARGLGKYAA *F FIVPQGREVEWIFSTPAGRKKLQDSANFQRLAVVTLHRDQVYSTLDEVKQELADSIKNLS *F PAGLTDQIPYLSLGSDVGKRETLICGFSKISGDFRIEEVEANGKTLRRLIFLSNQFVVQS *F EALVKTVKIKGKKDRKKIDFGYLACQHHLYMSVGVQLATTVQHPKRDVEKDVLVVGLGGG *F GLCSFLHAALPQARITAVEIDPIMLEVAEQYFELKQDKRFHVVIDDGLDFVERCRNEDIH *F FDAVLFDVDSKDLSLGMSCPPQSFLATKILQHIKEIIGPKGLFMLNLVCRDESLRTEALN *F NLHKVFPAVCSYKLEEDINEIIYCANDEKYKTVEQWKKNMGTAGRGLNSAVKETKLASED *F ALEVAEFLSELKI *F >38D.30 *F MERFLSGFSDCSCCFWLYDNVAAADSQIPSRAGDSAKDAARKTHIEPEEPAAPPRPEPPP *F PPLHNGAGVLLPSAQEGVPIVATSSSSSFAEVPQTGNRPISGDTSVPVKELPALSDQQRT *F KSHSEYISEIDLAADVGGASSSTAQVPNNQLNYNKRSSAGKVHRFSESQQLDQPKQLEQE *F EPATGQPKAPELQEPPQLPQAEESSQIVHGDQAIGTYANVADLQLRRIIYCIDKTADLLS *F MIAQRSARSVLSHVEMECNDRSYRLASMSKYLIDCERGTGIGGSEDRGAIVGAKAVSDTL *F CHPNAAVPRARNTRHHPYGPQNCLARKIYFPPLPPDGWRGGNLGDKFAGKMQPPLLTCAS *F AGRSEMIARCRSGCGCGFGLGCAEWCDRVHRYHAGGATSSSSSRRWVFGIASRSRIHYLV *F SSLSFFPEIITELPAVTITELPLDRVMNRLDSVILDGSPATAGNHSDEEQHQQQQQPHED *F QPMQVSEADEETNFDFSTCLILDEQMPKINDPGGGIQVEGMVTPEAATVGETQESSEELQ *F PGSAAFNGTEGTANLTNANEEVPMPVFSEWAQKQMEAEASREQAMELEQQVVNKSAQRKN *F NTGSSSGKPPTLKLRSKNYASPDCGAKIIAHNSESKHTEAVLTQSTDEYMLSTCESRIWF *F VVELCEAIQAQKVDVANYELFSSSPKNFTVAVSKRFPTRDWSNVGRFAAEDKRTIQTFEL *F HPHLFGKFVRVDITSHYANEHFCPLSLFRVFGTSEYEAFETEIRPSDDLDDFYDDYGAQE *F QKAAVGSGGNIFQSASDAVMQMVKKAAEVLVKPTKALKWSEESVLCQTPAFEAFSCINCN *F TTLVERINSLLSCQFQQLQALLSLSHLRSDLLNSRVCQEEFGISLTGSEFASKMGKEQSY *F FLSMLPAEHVGAMCKLIQAEQNVTDHNHTKAPSLKQHVSSPEPVQDNATATGVRQDCENS *F KTPTKEPLTPSLEVVVPEVSQEVPSMEDQSSTSSETVSTTNSTPADVNIFNMPSEPEEVV *F VKVQLPPEPTLPTTLQPSDVESFTDAPSTNALPGSSEAVANGDLGMEEGNPANWDGIDNL *F LTTTVASITAGGGAAAAAAAVVNGNANIGGAGIVGAGGPASLSSVNMQQKLTNGAQSESV *F FIRLSNRIKALERNMSLSGQYLEELSRRYKKQVEELQQTLTQQTLTVRQLEDQSRRYVEQ *F EQLYQQHSAELAGEVRALSYQVQACILVIIIVGTCIFLMLVLGTVYYRKLRRQQQQLLKK *F DQAGHPPVAAKPKLDRRKSYEQTPNQSTPKQRRPSEEAMLILKECGDSNMQELDPPSRQR *F KISVCYGSNNNIAANMAIANTNGGASVRNSLHRRKGAKHSWHNSLDTTETSCGEQTDKFF *F DVDTLKSIKQSCGKPGKKKSLQQLKPLGLKRQESAPATYTPDLQAEEPATQSDFDESLML *F DDDDLANFIPTSDLAYNEFMPEGPSGYQIVDTVDGKPGKEPGTKKSRRLSSPAFFKSPFS *F KSKNKGYSFNGVKNSHAVHEPTSWEWYRLKRSEKHQQQQQAKLVSKSLPSASLDSSSLSE *F VNFPLSSSTATTQNSFRILGEAILSSGEGRITPNGNGNAMSGGLASSSSGSGSGGSTTSS *F TTKKKQRALNNLFRKAFDF *F >38D.31 *F MSFLRGSVSYVWCTTSVLGKGATGSVFQGVNKITGESVAVKTFNPYSHMRPADVQMREFE *F ALKKVNHENIVKLLAIEEDQEGRGKVIVMELCTGGSLFNILDDPENSYGLPEHEFLLVLE *F HLCAGMKHLRDNKLVHRDLKPGNIMKFISEDGQTIYKLTDFGAARELEDNQPFASLYGTE *F EYLHPDLYERAVLRKSIQRSFTANVDLWSIGVTLYHVATGNLPFRPFGGRKNRETMHQIT *F TKKASGVISGTQLSENGPIEWSTTLPPHAHLSQGLKTLVTPLLAGLLEENREKTWSFDRF *F FHEVTLILRKRVIHVFFTNRTSSVEVFLEPDEQIDNFRERIFLQTEVPLEKQILLFNNEH *F LEKKVTPRTIDQPIFLYSNDDNNVQLPQQLDLPKFPVFPPNVSVENDASLAKSACSVGHE *F CKRRVDIFTSMDILIKKGVEHFIEMLVTTITLLLKRSEIKTLLTALENVKSDFDGAADVI *F SQMHKHFVIDDELNDQWTSSMHGKKCPCKTRASAQAKYLVERLRDSWQHLLRDRATRTLT *F YNDEQFHALEKIKVDHNGKRIKALLLDNVNPTVAQIAECLADWYKLAQTVYLKTQILEKD *F VRDCERKLNGIRDELYHVKSELKLDVDTKTINNNNQLAKIEERNRLRVMQQQQQEVMAVM *F RTNSDIISLLSKLGITNGSLESS *F >38D.32 *F MPPIRQLRSWRVTPIRVGPTNMYANSPPIARYSVIIIGLHRESCQQRNAHNRPKTPRSQE *F GKLPSWLHIFMTCLNNNAELGIQTVCLGSEKRRLHFAGKLVAQVTPPPSWQETNASVCVG *F SKTPN *F >38E.1 *F MEESNHGSAGCENVSQFMLDDLQLAAELGKTLLERNKELETFIKEYKIKGDEQELEILHL *F RKHINAMTEVNDSRLKVYEQLEVGIQDLERANQRLNLEKNRDKKQIKTLTTNTEVLEARC *F EELSQLLSDARQSLSTERRKVDQYQQERYRMQHSTEGSVSSHSIQSLCKEQSVEFSKLDV *F MAIANSTGLEDISFSNATMCERTAVKGEDNEELVKLLSEMEVLKRDFLAEQQRCTELEEQ *F LVTIIQDNQGLQTRLLENSANEGTMSMHEEFSLLDDVRQGQMCSRCLRDINESNTNMDDQ *F SSIAPTEEIYEDDDRSILSESTSKCDNSGADYKERFRIPEDLNPNSSDKPNPYRDLVEKY *F EALVEVKRTSNAVKSNFTSNPDGKTMTESSQGKKSETIVNSSKESDLMLDSTRKRTPTEF *F SESETTSSGFSDETSNKSTQTDERPSYFLCSISNGNDCKFSIYDDVSPIESHFRNRPEYR *F ELFKEIFGVLKKAADNNEEDKLPSLHDDAQITEKLPLVAAKVPPVTPEREESPDDFIDDT *F QSVVSSVISNQSIAMSECVTKLERKTAKKHIFDVRNQQNQSSLTQSTLLNSSSKETTVGE *F PNYKPIRENGQILTPLKREPLEYLTVGVGIKKKNRRKHRNQSSSGDRIELFNSREFTPRN *F SPLAMNNRGGGQGSSKMCTDTLNAEFGRSNRRRTTPSSSNWNGSPMVIYNKNMNTPQTSR *F GRVIELNGVEFYHNTVSQDLHKLKKLDLSYAEVLRRADAGEHGPTRSHSQRQQHNGANIR *F KSHHHQFRQK *F >38E.2 *F MLDALIRAAELVDLVLLRPLASVIDAVITGVYYFLWGSYLVGFCLVEGSRKGWNLLRCAV *F RNINEGIRDLGLITLDVADYFYGGTKGGLKNVLDFGHCISRFICNLLIDLGDGILWLLML *F LPRAILFLWDCLLDFVVHSIVAHGLSLLNSAFRASIGLALLLVLYMFRRYVYLMLIYLLQ *F RGRIEISTKTQSVYLWTHHKLQHFIHNVWYVSENAGSASPERCVVCMAQSRNVVVMPCRH *F LCLCKECSLQLVLLLEDRCPVCRHNITSFLSVYV *F >38E.3 *F MQIVKNDLAGAPRTLACILQREAAKIFIIIKIRTPATWSGRRSVPCSIGQHNLLHFMCRI *F KRRWRCDPRSGAAALTPAPPATEPRKIKPLGAGKLKVGKTDSNSDSNSNCDSRAAAAAST *F SATSATSATTLAATAAATAAAAEAGGAASAAAAAKISQVRLTNQATTSMLLLQPNSSFSS *F LSPFDNFSTQTASTTTTTSASAAGHHQHHNHLLHQQHHNQQQQQQQQQQQQQQQQQQQEH *F LQQQHQQQLVSPQQHLLKSETLLSHEEDQLISNLTDSSVVSHSELFSDLFFPSDSNNSLL *F SPTTSGYPDNPAEDLTSSIENLTKLTCLRDKRLSSIPEQQLSSEQEQQLCLLSLRSSSDP *F AIALHAQQQQQQQQQQQQQQQQHQQQQQHLQLQLISPIGGPLSCGSSLPSFQETYSLKYN *F SSSGSSPQQASSSSTAAPTPTDQVLTLKMDEDCFPPLSGGWSASPPAPSQLQQLHTLQSQ *F AQMSHPNSSNNSSNNAGNSHNNSGGYNYHGHFNAINASANLSPSSSASSLYEYNGVSAAD *F NFYGQQQQQQQQSYQQHNYNSHNGERYSLPTFPTISELAAATAAVEAAAAATVSSPSVGG *F PPPVRRASLPVQRTVSPAGSTAQSPKLAKITLNQRHSHAHAHALQLNSAPNSAASSPASA *F DLQAGRLLQAPSQLCAVCGDTAACQHYGVRTCEGCKGFFKRTVQKGSKYVCLADKNCPVD *F KRRRNRCQFCRFQKCLVVGMVKEVVRTDSLKGRRGRLPSKPKSPQESPPSPPISLITALV *F RSHVDTTPDPSCLDYSHYEEQSMSEADKVQQFYQLLTSSVDVIKQFAEKIPGYFDLLPED *F QELLFQSASLELFVLRLAYRARIDDTKLIFCNGTVLHRTQCLRSFGEWLNDIMEFSRSLH *F NLEIDISAFACLCALTLITERHGLREPKKVEQLQMKIIGSLRDHVTYNAEAQKKQHYFSR *F LLGKLPELRSLSVQGLQRIFYLKLEDLVPAPALIENMFVTTLPF *F >38E.4 *F MEADGEAQAKPEDEVETLGDLINGPGHICEKYTNGSRVRIGLVRSMGQRSLLILGQVFRF *F FFSTATGNMAAWQLGNLATWQLGGFDAIAFEIHDSDKARLMA *F >38E.5 *F MQMQDADANADIELTNYPWQKVSRHRYKKQLQHHAAQENNDNLPVDSSLSSDTQNGAKSI *F RCDTTRTGWFTFTLIASGLPVASWVLAPSGRLFGLRRKPILIYANCCELATSIGRQHARN *F AVKCDAEIIGIYLLLLIGSQNSLDLRIVGSHTVAIAVSASVSVSVAAALEMAKSSMLKAR *F DKRICHSSKHHSLSNCDRAGAFRPLTVNWASQLGPQSQGEWDIECIRLASALTSRVLFAT *F WQQPLATNSPAWLYRSPVNPKPRIVAVQSQGRHHFQVLWNRPSHKKKQTQSSDGKRRRVT *F SRTSAGGVCVCFVYPGGIYKCEIIVGGPNKNKSREERTPNGRPWEPRESREALSGRNEGV *F IGFNFRWQLLEKRGKESERERERESHGERAQPEIPRPSDDPDLALFHNDVTAPQQRREFP *F GGVSVAYIYVQCARPVVYSLPFPVCPCDDYAIKLTTGRGSLGSSIFPCSVLFLHADRTFR *F GPSVCQSNRCGSCNLAFQCLPHLQVRRRTRQRNAGWQTSCAPKHEINKPIDNVAGKNMEL *F LATLLENLKCTHADIQSLHVREPPAARQSKLTNNQQPTTNNRQQSGQNKRKTRRGETVRK *F ENLSAVQYLMSDELWRRLTEVLHSQAADHQMIMRMLLTWLVVLVVLLTAGGDGGGGGGGG *F ALMTSSWQWLHPPTDSTFFWHATDGTSTTPRDLWTLHSMGREWKWHRNRNQNKPSRRMLA *F EMQKHDR *F >38E.6 *F MAMGAKSARHRMTNQSKTNRRRTYDQRHSTPLRKCKPSPLTPPCTPVSPEQLHLPRPVVT *F FQVSFSLAKHMSKHTQTVAQVDRKALVG *F >38E.7 *F MRDRLASLIVVKQEGGSNTSISHHQATAIKCEASLYTESSLFQEINNNSCYRQNLNAPTH *F QQSHTSHLQHAQQHQTHQQHPLLPPPLPTLPLIYPCRNLFPDGCDINHLACCSSSNSNSN *F CNSDSNSTSSSPGNSHFFANGNTCMYLVFPFGLSVLVTFSFFVFLYFPNSNAAVVFSSNS *F LIAHAAYTQCAARIAKSAKKLTVCSIRLRRQVGKKSEKCNLNKKGGVAGGGRLDNAQ *F >38E.8 *F MLVNNNEENNNNAKPVQIFSPKIGDTRLENGKPEDAASTALPASTAQRTGSKPRFLTVVS *F VRTGDRGLCTWASPCGSRRQNVRSLSRPADCCVDRDRAERLGFLGAVALPFVFGYIFSHN *F AFARVSRLWVRLTLRPIVAPGFLAVVDGAVAVPLAIMVVAEVEVVVVVPVVLVGLVVLLL *F LADLLVAPKTATLKARTRTQPTPLSLRSNRHAIRQSAEGAIQRMSGMGHVVRLYIPKCID *F LYQLIVQNRTEQDRELGQQLKGNMQSRCLPAAFVKLKLSAHTLPLEIRRMEFQGAAQSRG *F TLTSPGTSSADNWPEMLAKRCSQSGWLDCLPSTCASNLQDSECGTVDRRVAAIAAADSHL *F RSHSSVINWTRSLSFLLLTQTSSANKATKFSSNRAAIECEIYCRRSIAWIELNRGFRTVS *F NIAYCSISVSIRLASKGPKCSDTTHTRLSAIIGVLMEHCEHCDDHKFRSSTSLWQPWGHD *F SSIQ *F >38E.9 *F MPKAKTHSRSVKTPFPVRSNRPGGVQKSEPSPSPSPLCRTSLLDLPEDIIRLVLEFLPRI *F TDKVLLACVAPKFRAAFEGWARVQRNALDMVSLETVPLPQLIRFFKVAGPFIRVLQVDCA *F SYQKESLLVEFVKEYCPNLEEISYSNATDEFHYRSIMSKMTHLKRVTIECLDAEDVLNFD *F MQPNQELEFFELVNGCYTGQNLCGFPNLKTLVLRDCLLWNSMEFGIPLKSLHTLDLDDCC *F FEVMNVSLYQKIAESCTNLVELIFSGCDTNFEVIANLPKLERCTLKTWMTSNELNIGFLT *F VLAEKRGNKLTHLHLSGQFNITNEHARCLGQLSSLTDLRFSNNDILDDDHFKFFNDLSQL *F ERFGLTACGRVMDVGMMRMLRKCPQLKVIDLTDCEQITEEFVIQAIGFCSKGSGRDVVLN *F VKGTMIRRPILTVGKPGREFRKHILTLSYFAASGLREFLESSKG *F >38E.10 *F MILLSGLLPLLGVVLLHLATPTQAFYLPGLAPVNFCKKTDVSSTCKSEILLYVNRLNTEE *F SVIPYEYHHFDFCLGEEQNSPVENLGQVVFGERIRPGPYKIQFLENQQCATTCVKTYKGD *F DPASNRRMMVLKKGISLNYQHHWIVDNMPVTWCYPLENGKQYCGIGFPMGCLVRSDGEGC *F PINSIYNQPMHYYPFNHVDLEITYHSGQSEDWGIQFGNSGRIISVKVTPKSIKHTDKENP *F NCLSTEPLAISENSLKAGEQLEIVYTYSVKFVKNDSIKWSSRWDYILESMPHTNIQWFSI *F LNSLVIVLFLSGMVAMIMLRTLHKDIARYNQMDSGEDAQEEFGWKLVHGDVFRPPRKGML *F LSVFLGSGVQVLVMAMITLAFACLGFLSPANRGALMTCSMVLFVSLGTPAGYVSARIYKS *F FGGLKWKSNVILTSIVCPGVVFSLFFVMNLVLWGENSSGAVPFSTLIALLALWFGVSVPL *F TFVGAYFGFRKRALEHPVRTNQIPRQIPDQSIYTQPIPGIVMGGVLPFGCIFIQLFFILS *F SLWSSQIYYMFGFLFLVFVILVITCSETTILLCYFHLCAEDYHWWWRSFLTSGFTAVYLF *F IYCCHYFVTKLSIKDSASTFLYFGYTAIMVFLFFLLTGTIGFFACFWFIHIEDNDGDEYD *F ELSELRQRHKPESQPSVDVHICETRSLIFSRFAVFFPGGLRSGRPSAHHWRIRQIPEALG *F FRNLPARLHTLFMADTPDDYWCRIPELLDLPLEQRKSLSIPKELDNDELVYSKCYTYGVN *F WTQLLESGEEDDLTTMEPNASWPLIKCPQGWEYNTSVVWSSIVIDFDLVCDQDIYPTIGL *F AALNTGGPVGVYLFGLLNDRGGRRLSYFVCLATLLAGSLMTSLSKDFWTWAGSRVIVGLT *F IPAVYQIPFIISLELVGENYRSFVTVMTCTFYTSGIMLLSGVTYLERDWVRLSYITSLPF *F YAYFLYMFVMPESPRWLLMRGRLEEALKILERMAKVNGREFPEAVHLKLEAQIRRDKLKK *F QKKKMANVGLADLCRTPNMRLKTILITLSWFANETVYLGLSYYGPALGTNQYVSFFLSAV *F VELPSYLCCWYFMDTWGRRWPLSLSMILGGVACVITVMLPDDAVDETLVLYLVSKALLSA *F SFLIIYPFAGELYPTQVRGIGIGASSYIGGLGLIGIPFITYLGKDNLKLPLVIMGFLSML *F GGMTGLRLPETLHHRLPQTIEEGEEFGKNWQFKDCCRCAQKPEILSQPASYENLDVLAGS *F STNASEVELELRDSRRVREPAPRIDERTPLDTTASGSGRPVHRPSMKRLVRQMSVMDTQR *F THDGTMQLTHWI *F >38E.11 *F MTSDIAMQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVAR *F MAAGLQLKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNILAALGTENTRPSCAAQCVA *F YVAVIELPINRWPMLIQTLVNKVVSEGSSEMHRESALEAIGYICQDIRFGVMENQSNDVL *F TAIIHGMRKVEPSNHVRLAATTALHNSLEFTKSNFEKDMERNFIMEVVCEATQCQDSQIC *F VAALQCLVKIMTLYYQYMEPYMAQALFPITLAAMKSDNDAVALQGIEFWSNVCDEEIDLA *F IESQEATDQGRAPQRVSKHYARGALQFLTPVLVEKLTKQDECDDEDTWSPAKAASVCLMV *F LATCCEDEIVPHVLPFIKENIESPNWRFRDAAVMTFGSVLNGLETNTLKPLVEQAMPTLI *F RLMYDSSVIVRDTIAWTFGRICDIIPEAAINETYLQTLLECFVKSLKSEPRVAANVCWAF *F IGLSDAAWEAAVTNDGETPETYALSPYFEYIITQLLETTDRSDGAQANLRCAAYQALMDM *F IKNSPLDCYLVVQRTTLVILERLNQVMQMETQINNHSDRHQFNDLQSLLCATLQSVLRKV *F HEQDAPQISDAIMTALLTMFNSSAGKSGVVQEEAFLAVSTLVELLGAQFAKYMPAFKDFL *F VMGLKNFQEYQVCCAAVGLTGDIFRALKDLMVPYSNEIMTVLINNLTEPTIHRTVKPQVL *F SAFGDIALSIGNHFLPYLSMVLDMLRVASNLQTDANNFDMNEYINELRESILEAYTGIIQ *F GLKGVDQTAHTDVMHMEPHLMHIISFIKRIAQEGDVSDSMLASAAGFIGDLCTSFGPRLY *F PLLDDAIITQFLAEGKRSKAQRTKMLCTWAVKEIKKINTQVITQ *F >38E.12 *F MALGLCEGMKTTRSVVGVPPNTNRPPHPVRRPDSLLPISEEPKSISSQTPNMDSGNDSAR *F PTPSPLAPTVSGISSLISTTFKPKDIMAFVEHLPTFDGTPRLLDRFITSVEEILMLIRGA *F DQTPYGLLTLRTIRNKIIDRADEALELANTPLVWDEIKSNLIRLYSSKKSEANLLSELNT *F FSDNLTLGQLFFGISKVRSQLFSILKNSEHNNTVVDAKKVVYNEVCLNAFMTGLKEPLKT *F FVRIKSPSTLEQAYEQCQIEQTLYRAQNKRTNRPEQGPNGSDNKTYRNSYDSNYRSGRND *F RNDRRGPYSNSNSNSNSGQNRPFNSHNRTPQSGTKDNRANTSNPFRAPSHSLNNIEENPQ *F PDSNFQQTASGNQQVLKYIKTSLYHFHRNIKSSYSPWSAPVWVVPKKITPTGEQKWRLVI *F DYRKLNEKTISDRYPIPNIADILDRLGKAKYFSTLDLASGFHQIEMNPDDTPKTAFTVEG *F GHYEFIRMPFGLKNAPATFQRVMDNIFGDLIGTICLVYLDDIIIFSTSLQEHFIHLKTIF *F GRLRSANFKVQLTKSYFLRRETEFLGHIVSQEGVRPNPNKIEAIKNFPCPHSKKSIKSFL *F GLLGYYRKFIRDFARLTQPMTQKLRGNNKSIIIDDEFKKAFEYCKTLLSNDPILQYPDFT *F KPFTLTTDASNFAIGAVLSQGPVHSDRPVCFASRTLSAAETNYSTIEKEMLAIIWAVQYF *F RPYLFGRRFTIITDHKPLTWLMNFKQPNSKIVRWRLQLQEYDFEVVYKKGSQNVIADALS *F RPEASVNHNEALSIPQNVCPISEKPLNDFNIQLLFKITPDTNNATLTPFKHKLRREFCKP *F NFQYDDVVCILRQSLKPNKTCAVFAPDHIFQMVEQAYQTYFSAHSQFKLIRCLIFLPEIT *F DSTEIEKIITDYHYNSNHRGIDETYLHIKRQQFFPHMKERITQLIRKCETCLKLKYDRQP *F QKITYQISELPSKPLDILHIDIYTINKNYNLTIIDKFSKFAAAYPITNRNCINVVKALKH *F FISQFGIPKKLIYDQGAEFASDMFNKFCTQFNIDLHVTSFQQSSSNSPVERLHSTLTEIY *F RIILDVRKQQKLSSEHDEIMSETLITYNNAIHSATKHTPFELFNGRTHIFNQTIQFNNEH *F DYLTKLNEFREKLYPLITDKLSNDVVRRTLKLNETRTDPVDLQPDTLVLRKENRRNKITP *F RFSIHKVKHDKGHTLITARNQKLHKSKIRKTVLKKDKSNNAIHVHYLNDNAPIAKIELGK *F ALLIERYKIISHVINLQDYSRCMEQFHLTINKFNPDSTLTDSVTILKTKLTQAQVKLKAL *F TPSYRNKRGLINGLGSLVKVVTGNMDANDNKEIHEELDNIKKNSEVSNDNLQKQVMFNNE *F ILIRFENITDHINNEQILISKFFDTSQNKIYKHLNLQDTLLEEIQYLNRINYNIELFINH *F LNDITESMLLAKINIIPKFILNEQEMDKIKTILEKQNITVKNEQSIYNFLQMNTLNYEQK *F IIFNIKVPIFKQPFHTLARLVPLPINNTYFVITPNYLAYNINNKKFHMTRKCPKLDNTFL *F CDENFYVDTPQNNTCLEHLLNGENSSCDVRETGPITDVFEAERGYIFAFNVNKLKVSLTN *F GSELSIMGSAIIRYINETIQINGIDYDGTVDTFPEQTDFDLPPMRKVTRNTTITVLSLEK *F LHLEATQTMDKILAVHHNTIQHTWTLYTLLGLVTFLAVILWLHRRTKHIVHIHEDHHVPI *F YASSIPSLWPSLRTGGGGVTTPPPKPPRL *F >38E.13 *F MPLKGIRVLEFVGLAPGPFCGKILTDFGATVTRIDKVMENPLDVLQQGKRTLCLDLKNPK *F GQQAVQRLVKKCDVLIEPFRPGVMEKLNLGPTDLCTANPRLIYARLTGFGQHGRLAQRAG *F HDINYAALSGVLSMLGRRHEKVTAPINILADFAGGSLMCALGICLALLERHRSGKGQVVD *F ASMVEGAAYVASWLFMSRNLVIWGRERGDNLVDGGSFFYDTYETKDGRYMSVGALEPQFF *F EMLKQRLELPEDVSQFGEEHQVRGRKLLTEAFLSKTQAEWSQIFEDVDACVYPVLDYREV *F HRHDHNIQRNSFEVTEDTATPRPAPVLSRTPGKLPKATDGAQVEWMDELDLKPDEFKDLL *F DSGVLSLPERAKL *F >38E.14 *F MPQQRRKVAFSGKKKKDQMLQKRNTKGPPKYLRSTQESYEDSDVPETTRKLMEQPFARGG *F NRNKNVNRYNLQFYQEGKKELEQMKQEGFKPFEKLSPAQREVDDRYFAGCDFPVRPPWTL *F TESKEELDRTENRYFKEYVDELQKKQRAGDSKELSLFELNLETWRQLWRVLEFSDILLII *F VDVRYATLMFPPSLYDYIINTLKKHAIVVFNKVDLVEPHAVVAWRQYFRDRYPQLPVVLF *F ASFLPRSRKGSQRGPQAHRRSMEGVYNIYKECQRYVQGEVDLTTWEQKIREDMRSDQLDI *F LDEISTAVEGELKISSSIDTTPHEHVKYHSGVLTIGCIGFPNVGKSSLINALKGRKVVSV *F SRTPGHTKHFQTIFLTPLVRLCDCPGLVFPSSTPKSLQVLLGSFPISQLAVPYRSLKFLG *F EHLNLPQLLRLHLPEDYDEWSAVAISDAWAYKRGFLTAKAARPDRYRAANHILRMCLAGQ *F QMLVLQFYPPGFEERREHWLQHPDVGEVKKYQQVELDEPESETNGDTSSVCSDTADSEDE *F DEDSSNDETNADEEECGIREDAPRSRNVFALLEDD *F >38E.15 *F MQRDRDSSGSNARKGNRPPGLRGKDIGLYYRNLARQQKKDRGENAESKEPQIRLGCNVSA *F PSGVLERVKELMEDYSRAPSRQNVDDKNVDAKFQQQFRHLLSVNFEEFVAETKERNADLD *F WVNPKLDERLQLELGQRQLEENAKKRLEARKKLPTMKYADDIIQAVRENQVILIVGSTGC *F GKTTQVPQILLDDAISRGCASSCRIICTQPRRISAIAIAEWVSYERCESLGNSVGYQIRL *F ESRKARERASITYCTTGVLLQQLQSDPLMHNLSVLILDEIHERSVETDLLMGLLKVILPH *F RPDLKVILMSATVREQDFCDYFNNCPMFRIEGVMFPVKMLYLEDVLSKTNYEFQKFRDRR *F PKRDPPERRMKHEAMIEPYLRRIRNSYDSRVLDKLRLPESEGCEDIDFIADLVYYICENE *F PEGAILVFLPGYDKISQLYNILDKPKTSKGQRWRDHMAVFPLHSLMQSGEQQAVFRRPPA *F GQRKVIISTIIAETSVTIDDVVYVINSGRTKATNYDIETNIQSLDEVWVTKANTQQRRGR *F AGRVRPGICYNLFSRAREDRMDDIPTPEILRSKLESIILSLKLLHIDDPYRFLQTLINAP *F NPEAIKMGVELLKRIEALDQTGTLTPLGMHLAKLPIDPQMGKMILMSALFCCLDPITSAA *F AALSFKSPFYSPLGKESRVDEIKRRMARNMRSDHLMVHNTIIAYRDSRYSHAERDFCYKN *F FLSSMTLQQLERMKNQFSELLYNYKKSRQIKNRVRAIHTMATDDGRRVNFHPSSVNSGES *F GFDSAYFVYFQRQKSTDLFLLDSTMVFPMALIIFGDGVEAGVTQNTPYLCVAKTYYFKCN *F RETADVVIQLRSNLEKLLLKKALYPAPIEENGYEKQLIKLVLTHVSLNNNNYVFISAQSH *F RIASLARRKTRRGLYFIR *F >38E.16 *F MSRHEKTKSTGGGLLDSLFGRPSKSKGGTISSGTLAHGGRPVSADNYVVPGVEDFEQYIQ *F QLSVAELDAKFLEIIEDMNIPKDKREPLLAKSKEERQKMIMWHLKGKNSLERSANSRFEK *F PIDYVEYLQNGEHSTHKVYQCVESLRVALTSNPISWIKEFGVAGIGTIEKLLARSKNNAS *F YEKIEFEAIRCLKAIMNNTWGLNVVLNPDQHSVVLLLAQSLDPRKPQTMCEALKLLASFC *F IVYERNGYEKVLRAITTIAATSFKASERFRPIVDALFASDQQDPKRDLACHSLIFINTLT *F NTPTDLNFRLHLRCEIMRMGLYDRLDEFTKIVEASNNENLQQHFKIFNEIREDDFEEFVQ *F RFDNVTFNMDDATDCFDVLKNLVTDTTSEPYFLSILQHLLYIRDDFYFRPAYYQLIEECI *F SQIVFHKGYCDPNFENRNFNIDTSLLLDDIVEKAKAKESKRSEEYEKKIEQLESAKQEAE *F AKAAHLEEKVKLMEANGVAAPSPNKLPKVNIPMPPPPPGGGGAPPPPPPPMPGRAGGGPP *F PPPPPPMPGRAGGPPPPPPPPGMGGPPPPPMPGMMRPGGGPPPPPMMMGPMVPVLPHGLK *F PKKKWDVKNPMKRANWKAIVPAKMSDKAFWVKCQEDKLAQDDFLAELAVKFSSKPVKKEQ *F KDAVDKPTTLTKKNVDLRVLDSKTAQNLAIMLGGSLKHLSYEQIKICLLRCDTDILSSNI *F LQQLIQYLPPPEHLKRLQEIKAKGEPLPPIEQFAATIGEIKRLSPRLHNLNFKLTYADMV *F QDIKPDIVAGTAACEEIRNSKKFSKILELILLLGNYMNSGSKNEAAFGFEISYLTKLSNT *F KDADNKQTLLHYLADLVEKKFPDALNFYDDLSHVNKASRVNMDAIQKAMRQMNSAVKNLE *F TDLQNNKVPQCDDDKFSEVMGKFAEECRQQVDVLGKMQLQMEKLYKDLSEYYAFDPSKYT *F MEEFFADIKTFKDAFQAAHNDNVRVREELEKKRRLQEAREQSAREQQERQQRKKAVVDMD *F APQTQEGVMDSLLEALQTGSAFGQRNRQARRQRPAGAERRAQLSRSRSRTRVTNGQLMTR *F EMILNEVLGSA *F >38E.17 *F MHLLRDRLGVAGGRKGFLEGFGPANGRKTLKNVKRTDKQRMPHDNDTSTIHTYPSIHTQI *F RLASTLHQATDIGNGVLHDLPVPGMRRGAQPYRAHGSYPSRPSPRSKFHRIVDGLRKRGK *F NKSGLWLPNCCVNFMGRSMGRSTHQHHLTGLVWCPRSRATSTIASWFLYSGLWVAMAMVM *F AKAIAFA *F >38E.18 *F MSSANSDSADKETEPNIFPLQLVAIFDGLSWDRERYWYSERDPDQNRTTKPLKQKYTASA *F SSDNRGCRFIATPRKLKWAFDVPCRHMPPHAAQTTENGPFIILSKITKSGLAAINANCMP *F TDFMGLMDIGVIVDIVDIVVFIFPFLTCARVINREQMCKRILIKAHICTVRDGQGKARTA *F RAWREDNAVHYVLGNMESSGSECSQADAGETQAQGGLLHPVEVWRLRWRCPVVVAHRIVI *F VVVVMGGWAIVVVVVVVVVVGDGTQMVCQVMMRRRGRSRRPRARDFRARNGNRWRRQTFG *F DVLLMVGWCGTWSSIGGPANTTATACSSDADAAVGLVELGRTRCTTATAAPGTGGTAGGM *F GVMMVVLLHQLVGRRGHILNEVIRRARGGGRRRRVEHVMGVVVHLVGSGRVVGGMRGVHI *F AQELVVRRRVVVVGRPVPWLARR *F >38E.19 *F MAGGNFQQRNGVSKSQVLRFVCPNLRGAIDEIALSAGVRGLPPRHKTIALIFRQKNRLNS *F SGADLWKARFSRVGPETTIRNQDVTGVMSSALSDKLTPSGCCLRVITEPSESNQLPPRTF *F DEWGTATKAYRDKCPTIPELYSKTRTKDKYRVVYTDFQRLELEKEYCTSRYITIRRKSEL *F AQTLSLSERQVKIWFQNRRAKERKQNKKGSDPNVMGVGVQHADYSQLLDAKAKLEPGLHL *F SHSLAHSMNPMAAMNIPAMRLHPHLAAHSHSLAAVAAHSHQLQQQHSAQMSAAAAVGTLS *F >38E.20 *F MNTIDKATQWNFNFLLGLIRVNVHSYAPPTKSNNNNKCGKRQSTNRPFSLPNRSPPHSAI *F TVIGSTVGSLALTQIDIRVSLATAFRRQSEIIITSQLNLGNVCDYLCKCRCIKLSRNTTK *F TTYNKAVVMSFKRSSSVILPQLQPSSSYLNWHPEEVARQSGSDPYGYKKKERYRIAPGLV *F RHNAQSSRRPSAYSCSLFSDRRIPIPEPLTKRKPLAIQYQPSLKVQPAEVSVLNATGRVG *F MPTEANCLNQLAHVHRLRDSELNYNEHQYNRNMQMLRNHEGLGVPLKMGMERFAARQVGR *F LPFLSSSNFMDDVLTGRCDSIGFEDFMNLPENSEHMRQPHAVVEKSLGIYQ *F >38E.21 *F MYRMQQVTRIEISKTFTGNHQNAQREVEYRTRLSQLLSSDICSISFSSGCVGVAALFGEQ *F AYRARELRAFKSPRTKPDHIRSRMATDTTHNSEGNQNMSKTYTNGYFRKLVGTGGRSCCR *F LLGWRLMEPKEAAADGRNVSRRLITGTDADADAASSFKMVRWSARSRRQARQDKVELLAR *F NNKVNGEDDTSDNAAFRNSTDLSDHDEIFLKGLLRSNSNTPHKELMASRPEHINLVSQLV *F ANSAKLNPTEPQPVPLFLPAHLNNKPICDDIIRKFSPSRRLESRELAKLLAQPHFRALLR *F AHDEIGALYEQRLKAAGGSTSQLEIASQRQTGGYLFTEDVLNTKMPVETIKMVGLRRDPS *F KPLGLTVELDEFKQLVVARILAGGVIDKQSMLHVGDVILEVNGTPVRTPDELQVEVSRAK *F ENLTLKIGPNVDEEIKSGRYTVSGGQVKQNGIASLETGKKLTCYMRALFTYNPSEDSLLP *F CRDIGLPFKSGDILQIINVKDPNWWQAKNITAESDKIGLIPSQELEERRKAFVAPEADYV *F HKIGICGTRISKRKRKTMYRSVANCEFDKAELLLYEEVTRMPPFRRKTLVLIGVSGVGRR *F TLKNRLINSDVDKFGAVIPHTSRPKRALEENGSSYWFMDREEMEEAVRNNEFLEYGEHNG *F NLYGTHLQSIKDVINSGRMCILDCAPNALKILHNSQELMPFVIFVAAPGMEQLKTIYADR *F RATGSNRNLSATLVALLDLCLNTQTLLNTFAFYDFSEQDDDLVATVEESSFVQRKYEKYF *F DMVIVNEDFDETFRQVVETLDQMSHEEQWVPVNWIY *F >38E.22 *F MHLMPGDADFSESFAAWGQTEFQGKEEADADAPTKSPRERDGCTKIEREHESEASEKLLP *F HMCSSLTHMEACCAQFSSRVYVWREGVKAGVWRGEPLDCASENSFYDDGHHTAFRHSLAD *F ADNAAGTMMSMTSSPPATPTGVQTDSDGLILPKKLINPCMENIDRKELHRELKFNARVGK *F SVLNQKSELQRAYDKQKERHAAAEQHSPDADIVGGIPGLKGELGRVIMERAQKHEAAAQK *F QDQEDAEERQYVNPEYLNVRAKLRTAHANN *F >38E.23 *F MISISTILALVVSSVWATDYTLEFEDSDLYSECSEKLPGAIGLREAFDMRNIVTELDIDG *F LHLSGNCTTIWDVPSTDRISLRMTVMHFDRGTWQPTVFNTYARDFCAVMFDKELSWYKYW *F LKYFANREEISEKCIGTKGTVLVYKPFIVKPLIQNVIGPIYRGRVKAIFNFESFDKNNVK *F GATDVCFEVRGRINDLFSQGKMIRGLVILVLALANTWATDYNALIDDEGIYVKCSEAPAG *F TLGPRDVFNIDNMVMHMEPEGIYVSGNMTVKLNFLPSDRISARFSVMHYERGSWQPTMFN *F LHSPNFCEVMFDEDQYWFKYWFRYIRNKEEIREKCLKVKDTVLVYDEFLMVLHLENVNTS *F NLQGRYKAVITLEAFDEHNVRRPSSLCVEIRGDLERKTQFTIDKMNRKHNKISHSGSHMH *F GIKVEPKKYSPQEYREKILSLFPLPPRPANMCHDAFVKKLLEPYVLETKEVVRAVKMTRD *F STGEDSSEDTYEAFRAKMYKTNYTARATVYMARATILARTSVKSILSPEQRDSAAQTTQM *F FKVRFANLISDFRNVCIIYQLLQLQEILNVMREYPPAGTNPNDTIFGWSYKENLKRFEQQ *F PSTVYVDILEKNKPEATLDDLKFYVDLGELIGLVQALLDDVIHDRDLCAPNGFMELLKDT *F QVDIDKLIALPYETIMAEHDSLLEEKEKKNRVGKFHNPKLTAKQHALNKERFQIDYMSPI *F ESRYKVNWTHDVVDQGQYIDFLRCKVLSDELEKIEELMRTDSSVWRSCHLEYVTLIEQYK *F QRINKIQQDYDDDMETAENKLQATLNRVGKCKEDLRSCQEKVEEFHVKIEEVREKIAKEV *F EKERARLSAARVSKRMSRILARQKEEKKAAAQQAKLDKKLARQNKKSQE *F >38E.24 *F MLSFREPENRERQRRTANTPLNSGSIIMKTARNARAEKRQQAAVFCRIGFYLWAALSRSR *F SCSRCSSFLFCPFCPCRFVVFVISMSVCPVPLTRTVEYVWVFGVSPYELRISLDVIYEFL *F ITAYFVVLFALLTLVLITWLPIWVGLMIDKLSLMSSDTKKKAATPASYLKRKSRARHSIR *F FLARSSRSVKGKPGYNERTVYLGELYLQDVDLISF # *U FBrf0139866 *a Whitfield *b E. *t 2001.10.8 *T personal communication to FlyBase *u FlyBase error report for CG4807 on Mon Oct 8 08:06:16 2001. *F Date: Mon, 8 Oct 2001 08:06:16 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG4807 on Mon Oct 8 08:06:16 2001 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG4807 *F Release: 2 *F Gene annotation error *F Gene CG4807 has incorrect exon/intron structure or translation start site. *F Comments: Genome translation of ab (AE003631; AAF53087) does not extend to the *F first *F encoding Met of the first exon. *F AAA86639 MTESTQLQTAENNNAGVVKMEPPPPATSSVSVSAAAAAHALSSLSSLTMAATGSALSPAT *F AAF53087 \------------------------------------------------MAATGSALSPAT *F Comparing translation to that from Hu et al, Genes Dev. 9:2936-2948(1995) *F (U43733; AAA86639) shows the genome translation lacks first 48 aa that are *F upstream and in-frame to the currently annotated initiating Met. *F CDS should start from 182854, not 182998 \- then all will be well! *F thanks # *U FBrf0139867 *a Manning *b G. *t 2001.10.8 *T personal communication to FlyBase *u *F Date: Mon, 08 Oct 2001 12:07:53 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: PKC Delta *F We have identified a Drosophila Protein Kinase C Delta gene (PKCd) by *F analysis of the public genome sequence. Phylogenetic analysis implies that *F this is an ortholog of the PKC delta/PKC tau group of mammals, with closest *F homology to PKC delta. The prediction was made using a HMM search for *F kinase domains in the Drosophila genome, followed by genewise predictions *F using close homologs. It is complete at the C-terminus but is missing about *F 142 AAs at the N-terminus, by comparison with mammalian homologs. The *F missing N-terminus is conserved between PKCd and PKCt but similar sequences *F cannot be found in any available Drosophila sequences. The genomic sequence *F contains 97 AA of upstream open reading frame, but has no initiator *F methionine and no clear homology to PKC genes. *F This gene shares a small overlap (114 nt) with predicted gene CG10524, *F which has no other PKC homology, and may be a misprediction. PKCd contains *F a large (13.3kb) intron which contains another predicted gene, CG11245; *F this intron occurs in the middle of a C1 domain on PKCd, so the two genes *F are unlikely to be alternative splice forms of each other. CG11245 is not *F supported by ESTs and has only poor homology to known proteins. *F No ESTs are known from this gene, indicating selective expression in space *F or time. *F Fly orthologs of all mammalian PKC subfamilies can now be determined: PKC *F alpha/beta/gamma are orthologous to inaC and Pkc53E; PKC epsilon/eta are *F orthologous to Pkc98E; PKC iota/zeta are orthologous to CG10261 and PKC *F delta/zeta are orthologous to this PKCd *F \-Gerard Manning. # *U FBrf0139868 *a Manning *b G. *t 2001.10.9 *T personal communication to FlyBase *u *F Date: Mon, 08 Oct 2001 17:01:51 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: gasket *F We predict a novel member of the glycogen synthetase kinase 3 family, *F gasket (gskt = glycogen synthetase kinase three) based on genomic sequence *F analysis. gskt consists of a single exon, mapping to 100C1-3, which encodes *F a protein with clear homology to shaggy/zeste-white 3, and the vertebrate *F genes GSK alpha and beta. gskt and sgg are more similar to each other than *F either is to GSKa/b, indicating that an ancestral gene duplicated *F independently in the fly and vertebrate lineages. *F sgg has three known splice forms; one core short form, and longer forms *F that extend either the N or C termini. gskt aligns from end to end with the *F short form (sgg-P2). The N-terminal kinase domains (75% identical AA *F sequence) are followed by a region of ~55% sequence homology, and a *F divergent ~140 AA C-terminal tail with ~25% sequence identity. This last *F region is not supported by EST sequence and may be mispredicted, as sgg and *F human GSK3 share some similarity in this region. *F 21 ESTs cover this open reading frame and extend 5' to give a 130 nt UTR, *F as well as covering from residues 1-337 of the 501 AA open reading frame. *F 19 ESTs are from the AT (adult testes) library and 2 from the GH (adult *F head) library. *F Vertebrate GSK3 genes are involved in insulin signalling: phosphorylation *F and inactivation of GSK3 by ATK blocks the inhibitory phosphorylation of *F GSK3 substrates including glygogen synthetase and eIF2B. sgg has been well *F studied in wg signaling, but no role in insulin response has been *F described; it may be that gskt has some role in this process, or in *F signalling downstream of frizzled receptors other than the fz2 wingless *F receptor. *F \-Gerard Manning. # *U FBrf0139869 *a Manning *b G. *t 2001.10.10 *T personal communication to FlyBase *u *F Date: Wed, 10 Oct 2001 19:48:17 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: STLK *F Genomic sequence analysis predicts a novel fly kinase: Stlk (Ste20-like *F kinase) is a member of the Ste20 (MAP4K) family of kinases that also *F includes Drosophila genes msn, mbt, ninaC and DPak, and is most similar to *F STLK5 and STLK6, two yet-to-be-published human kinases, and the Y52D3.1 *F gene of C. elegans. *F The peptide sequence has been published in TrEMBL (accession P83098). It *F was created by a HMM search of the genome sequence followed by gene *F extension using genewise with the human STLKs as templates. The sequence is *F partially confirmed by 10 ESTs: 9 from embryonic sources (7 from the LD *F library, 2 from RE) and one from ovary (GM library). The peptide reading *F frame is open at the C-terminus and probably incomplete; two of the ESTs *F have mate pairs (LD05623.3prime and LD01213.3prime) which map to the *F genomic sequence starting 1.5 kb downstream of the end of the peptide and *F probably are part of the 3' UTR of the gene. These sequences may be useful *F in designing RT-PCR primers to find the C terminus of the gene. The gene *F has three exons and maps to 41F1. *F \-Gerard Manning. # *U FBrf0139870 *a Davis *b T. *t 2001.10.11 *T personal communication to FlyBase *u FlyBase error report for CG13419 on Thu Oct 11 08:13:02 2001. *F Date: Thu, 11 Oct 2001 08:13:02 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@cardiff.ac.uk *F Subject: FlyBase error report for CG13419 on Thu Oct 11 08:13:02 2001 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: CG13419 *F Release: 2 *F Missed gene *F Comments: CG13419 has a significant homology with the gene CG15284 in the *F cystine-knot domain 20/72 identity(27%), 31/72 similarity(42%). CG15284 is *F also a putative cystine-knot domain protein. Both have good homology in this *F domain with human Norrie's disease protein. # *U FBrf0139871 *a Pace *b N.R. *c S. *d Marquez *e J.K. *f Harris *t 2001.9.28 *T personal communication to FlyBase *u FlyBase error report for Ribonuclease P RNA on Fri Sep 28 10:51:04 2001. *F Date: Fri, 28 Sep 2001 10:51:05 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: marquezs@colorado.edu *F Subject: FlyBase error report for Ribonuclease P RNA on Fri Sep 28 10:51:04 2001 *F Error report from Dr. Norman R. Pace, Steven Marquez, J. Kirk Harris *F (marquezs@colorado.edu) *F Gene or accession: Ribonuclease P RNA *F Release: 2 *F Missed gene *F Comments: We have blasted the D.melanogaster database with conserved sequences *F found in Ribonuclease P RNA subunit. This RNA has secondary structure *F consistent with other eucaryal RNase P RNAs. Based on database sequence, we *F have PCR amplified this gene from genomic DNA and it's sequence matched the *F sequence in the database. We have not identified the exact 5' and 3' termini *F of the gene. D. melanogaster RNase P RNA can be found in AE003777.1 position *F 55063-54764. Here is the sequence: *F AGTCAGTTGCAAACTAGCATCTGGGGCCCACACAACGAGTATCTGATTACTCCACAAACCATTGCCCCGGGAAGGTCTG *F AGAATCGGCCGAGCCAGCTGTTTGTTGCGGCTTCATTTCCCAGCAGGAAACCTGTGTGATTGCAGGGCGAAAGTACCAG *F AAATCCTGCTACCAGGTGTTGCCGTTGCCCCCGGTGACCGCCGCCTGGTTGGCATTGAAACCTTTCGTGGCCAGCGTTT *F TTAGTGCGATGTGCTTGCTGCCTCTAAGGCAGAACTCAATTCAGACTAATCTGTGACTGACT # *U FBrf0139872 *a Manning *b G. *t 2001.10.11 *T personal communication to FlyBase *u *F Date: Thu, 11 Oct 2001 10:37:39 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: Haspin *F We have identified a Drosophila Haspin kinase by analysis of genomic and *F EST sequence. A HMM search of the genome for kinase domains was followed by *F gene extension using EST sequences and genewise. The protein sequence *F (TrEMBL: P83103) is complete with UTRs at both ends. It encodes a divergent *F protein kinase domain that bears strong similarity to the Haspin gene of *F mouse and human and has likely orthologs in S. pombe, C. elegans (three or *F more genes), Arabidopsis, S. cerevisiae and a number of other vertebrates. *F All these genes have a conserved C-terminal kinase domain and a divergent N *F terminus. *F Mouse Haspin is selectively expressed in testicular germ cells and has *F shown biochemical kinase activity (Tanaka et al, JBC (1999) 274:17049-57). *F Overexpressed mouse haspin localises to the nucleus where it can bind DNA *F and block cells in G1 phase, in wild type or mutant form where the kinase *F ATP-binding region has been deleted. The fly gene has a predicted nuclear *F localisation, by the PSORT II prediction, but does not contain the weak *F leucine zipper found in the mouse gene, and so might not directly bind DNA. *F 19 ESTs are known from this gene, from 17 clones derived from embryo (14 *F LD library clones and 4 RE library clones) and ovary (1 GM ovary library *F clone), so expression appears to be much higher in embryo than larva or *F adult. The map location of this gene is still unclear; the sequence is *F derived from two different genomic contigs (|gb|AE003029 and gb|AC005334). *F The first is unmapped, and the second contains the spen gene which maps to *F 21B2-4, but this may be a chimeric contig. *F \-Gerard Manning. # *U FBrf0139873 *a Manning *b G. *t 2001.10.11 *T personal communication to FlyBase *u *F Date: Thu, 11 Oct 2001 11:52:54 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: TAKL *F We have identified a Drosophila kinase called TAKL1(Tak1-like 1) by *F genomic sequence analysis, using a profile HMM to find kinase domains, *F followed by genewise to extend with homology-based gene prediction. The *F C-terminal end was created by extending the sequence from the end of clear *F homology to the first in-frame stop; in the absence of transcript data, it *F should be taken as tentative. The protein (TrEMBL: P83104) is most closely *F related to Drosophila and mammalian Tak1 genes as well as the Tak1-like fly *F predicted gene CG4803, which belong to mixed lineage kinase (MLK) family of *F kinases. The gene maps to 92F5-8. No ESTs have been sequenced from this *F gene, indicating a restricted expression in space and time. *F \-Gerard Manning. # *U FBrf0139874 *a Manning *b G. *t 2001.10.11 *T personal communication to FlyBase *u *F Date: Thu, 11 Oct 2001 13:23:57 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: p38c *F We have identified p38C, a third member of the p38 MAP kinase family, by *F analysis of genomic sequence, using a profile HMM to detect kinase *F sequences followed by genewise to extend the initial sequence by homology *F and genomic walking to find start and stop sites. The protein (TrEMBL: *F P83100) aligns along its full length with fly p38A (Mpk2) and p38B (53% *F identity, 72-73% similarity), and also with mammalian p38 alpha-delta *F (39-44% sequence identity, 62-64% similarity), suggesting that a single *F ancestral p38 gene may have duplicated independently in the insect and *F vertebrate lineages. C. elegans has two p38 genes (F42G8.3 and B0218.3) *F which are slightly more similar to each other than to fly or human p38s, *F again arguing for lineage-specific duplications. *F The sequence maps as a single exon at 91E1-2; curiously p38A/Mpk2 also *F consists of a single exon and maps 462bp upstream from p38C, indicating *F that these may have arisen by genomic duplication. The two DNA sequences *F are 61% identical in their overlap; the conservation of peptide open *F reading frame despite DNA sequence divergence argues against p38C being a *F pseudogene, though no ESTs have been sequenced from this gene, and the *F putative promoter region is small. ESTs encoding a putative seryl tRNA *F ligase start 123 bp downstream of the p38C stop, further showing crowding *F of genes in this area. *F \-Gerard Manning. # *U FBrf0139888 *a Manning *b G. *t 2001.10.15 *T personal communication to FlyBase *u *F Date: Mon, 15 Oct 2001 14:58:15 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: WSCK *F WSCK is a predicted novel receptor tyrosine kinase. It was discovered in a *F computational screen for kinase domains in fly genomic sequence; the *F initial HMM hit was extended using Genewise gene finding and overlapping *F ESTs, giving a full open reading frame ((TrEMBL: P83097) and flanking UTRs *F . The 2980nt predicted transcript encodes an 809 AA protein with 5' and 3' *F UTRs and a polyA tail, if a single base is inserted into the genomic *F sequence to correct a predicted frameshift. *F The sequence predicts a receptor-like protein, with a signal peptide from *F 1-32 and a single transmembrane domain in the region of 418-463 (different *F methods predict different co-ordinates within that region). The putative *F extracellular region includes a WSC domain from 42-115, a full fibronectin *F type III (FnIII) repeat from 129-233 and a partial FnIII repeat from *F 248-286. The WSC domain has been weakly ascribed a role in carbohydrate *F binding. FnIII domains are common in extracellular regions of cell surface *F proteins, especially receptor tyrosine kinases and cytokine receptors and *F may bind heparin or other cell surface proteins. *F The putative intracellular region has a divergent kinase domain from *F 511-770 which is most similar to tyrosine kinase domains from the tie/tek *F and FGF-R families of receptor tyrosine kinases, though the similarity of *F kinases within these families is much higher to each other than to WSCK. *F The divergence of this sequence cautions against strong conclusions from *F the homology or that this is a fully functional kinase domain. However, the *F relationship to FGF receptor kinases may be relevant to the carbohydrate *F and heparin-binding properties of the WSC and FnIII domains, given that FGF *F binding to receptor is dependent on heparin binding; WSCK could *F speculatively be a FGF co-receptor. *F The sequence is almost completely covered by 15 ESTs (excluding the *F frameshift region), from a variety of libraries: 5 from embryo (LD *F library), 5 from head (1 from GH library, 4 from RH normalized head *F library), 1 from ovary (GM), 1 from larval/pupal stages (LP) and three from *F S2 cells (SD library). *F The sequence maps as a two-exon transcript to 96A13-16, between predicted *F gene CG6668, a conserved novel protein, and Syx 18, encoding a t-SNARE. *F \-Gerard Manning. # *U FBrf0139889 *a Manning *b G. *t 2001.10.15 *T personal communication to FlyBase *u *F Date: Mon, 15 Oct 2001 12:18:58 \-0700 *F To: Gillian Millburn (Genetics) *F From: Gerard Manning *F Subject: Novel kinase: Dyrk3 *F Dyrk3 is a novel Drosophila kinase belonging to the Dyrk family. It was *F discovered in a computational screen for kinase domains in fly genomic *F sequence; the initial HMM hit was extended using Genewise gene finding and *F overlapping ESTs, giving a full open reading frame and flanking UTRs *F (TrEMBL: P83102). *F The sequence is most similar to human Dyrk2 (75% identity in kinase domain, *F 72% in a 397 AA extended overlap, excluding the divergent first 202 AA and *F the last 25 AA of fly Dyrk3). There are two other Dyrk family members in *F Drosophila: mnb (Dyrk1) and smi35A (Dyrk2), and two in C. elegans (F49E11.1 *F and T04C10.1). mnb and T04C10.1 are likely orthologous to human genes Dyrk1 *F and mnb, while Drosophila Dyrk3 and F49E11.1 appear to be orthologous to *F human Dyrk2 and Dyrk3. smi35A and human Dyrk4 are outliers to this group, *F whose orthology is not clear. *F 18 ESTs cover most of the sequence, including 15 from embryo (14 from the *F RE normalized library, 1 from the LD library), ovary (1 GM library clone), *F adult head (1 HL clone) and S2 cells (1 SD clone). 10 of the RE ESTs show *F alternative splicing, with an extended first exon and missing second exon, *F but this is in the 5' UTR and does not affect the protein sequence. The *F single S2 clone (SD25722) also skips the second exon, and extends the first *F exon even further. *F Dyrk3 maps to 102EF; a partial sequence of this gene including an intron *F has been published to Genbank (gi|5081375) as 'unknown telomeric protein *F gene' and annotated as mapping to telomeric heterochromatin. This is the *F site of insertion of a hsp70-white+ transgene, stock 118E-15 in Cryderman *F et al (EMBO J. (1999), 18:3724-35). *F \-Gerard Manning. # *U FBrf0139891 *a Isono *b K. *t 2001.9.22 *T personal communication to FlyBase *u FlyBase error report for CG15779 and CG3171 on Sat Sep 22 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 22 Sep 2001 22:04:33 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: isono@bio.is.tohoku.ac.jp *F Subject: FlyBase error report for CG15779 on Sat Sep 22 22:04:33 2001 *F Error report from Kunio Isono (isono@bio.is.tohoku.ac.jp) *F Gene or accession: CG15779 *F Release: 1 *F Missed gene *F Comments: CG15779 (previously named as GRLU.7 but presently given an unified *F nomenclature as Gr5a) is identified as Tre (Trehalose sensitivity). Please *F note that the adjacent gene CG3171 is mistakenly annotated as Tre in this *F database and other genome databases by the following publication: *F Ishimoto H, Matsumoto A, Tanimura T: Molecular identification of a taste *F receptor gene for trehalose in Drosophila. Science 2000, 289:116-119. *F Please see our publication: *F Ueno K, Ohta M, Morita H, Mikuni Y, Nakajima S, Yamamoto K and Isono K: *F Trehalose sensitivity in Drosophila correlates with mutations in and *F expression of the gustatory receptor gene Gr5a. Curr Biol 2001,11:1451-1455. *F Please also find genbank accessions AB066610-AB066643 for mutations and *F polymorphisms of CG3171 and CG15779. Thank you. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 22 Sep 2001 22:20:00 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: isono@bio.is.tohoku.ac.jp *F Subject: FlyBase error report for CG3171 on Sat Sep 22 22:20:00 2001 *F Error report from Kunio Isono (isono@bio.is.tohoku.ac.jp) *F Gene or accession: CG3171 *F Release: 1 *F Gene annotation error *F Gene CG3171 has a mistake in the supporting evidence or functional assignment. *F Comments: The adjacent gene CG15779 (previously named as GRLU.7 but presently *F given an unified nomenclature as Gr5a) is identified as Tre (Trehalose *F sensitivity). Please note that CG3171 is mistakenly annotated as Tre in this *F database and other genome databases by the following publication: *F Ishimoto H, Matsumoto A, Tanimura T: Molecular identification of a taste *F receptor gene for trehalose in Drosophila. Science 2000, 289:116-119. *F Please see our publication: *F Ueno K, Ohta M, Morita H, Mikuni Y, Nakajima S, Yamamoto K and Isono K: *F Trehalose sensitivity in Drosophila correlates with mutations in and *F expression of the gustatory receptor gene Gr5a. Curr Biol 2001,11:1451-1455. *F Please also find genbank accessions AB066610-AB066643 for mutations and *F polymorphisms of CG3171 and CG15779. Thank you. # *U FBrf0139892 *a Gornek *b M. *c J. *d Deal *e K. *f Cook *t 2001.10.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Oct 02 19:29:35 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Isolation and characterization of Df(2L)BSC7 *F Isolation and characterization of Df(2L)BSC7 *F Millie Gornek, Jennifer Deal and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2L)BSC7 was generated by irradiating males bearing *F P{lacW}l(2)k14206k14206 with 4000 rads from a cesium source. Polytene *F chromosome squashes indicated that the deletion has breakpoints *F 26D10-E1;27C1. Df(2L)BSC7 fails to complement eyacli-1 and *F P{lacW}l(2)k13315k13315. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0139893 *a Roote *b J. *t 2001.10.9 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Tue Oct 09 11:18:49 2001 *F To: rd120@mole.bio.cam.ac.uk *F Subject: I707 *F from Cambridge lost stock list: *F 123-11 w; Tp(2;3)I707 \+ In(2L)Cy, Cy TE45F wa/In(2L)Cy, (ap ast) b *F cyt: Ising: 46D;49A;63A-B *F syn: Tp(2;3)I707 *F origin: Ising 7/90 *F sent to: Irvine,Princeton,93-09-01; *F discarded 2/97 # *U FBrf0139894 *a Cook *b K. *t 2001.10.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Oct 09 19:13:18 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Cytological breakpoints of Df(2L)gamma7 *F Polytene chromosome squashes showed that the breakpoints of Df(2L)gamma7 *F (FBab0022171) are 30A9-B1;30D2-F4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0139895 *a Kao *b L.R. *t 2001.10.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Oct 10 19:27:09 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Df(3R)LK19-1 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Ling-Rong Kao, Indiana University (9/01). *F Df(3R)LK19-1 was recovered as a P transposase-induced male recombination *F event between a chromosome bearing P{PZ}l(3)0569705697 and an e1-marked *F chromosome. The deletion breakpoints were determined from sequences *F flanking the P element before and after the male recombination event. The *F sequence flanking the 5' (distal) end of the original P{PZ}l(3)0569705697 *F insertion is *F 5'-TTTGTAGTTGAAAAATATGTTCGCATCTGTGGAATTAAGCTCATATCGCGATC-3' *F as determined from sequencing the inverse PCR product following Sau3A *F digestion and ligation. This sequence falls within genomic scaffold *F AE003721. The sequence flanking the 5' end of the P element in the *F deletion chromosome is *F 5'-GGCGGCTGTGCTGATAGAGAAGAAGAGGAAGAAAGATC-3' *F as determined from sequencing the inverse PCR product following Sau3A *F digestion and ligation. This sequence falls within genomic scaffold *F AE003722. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0139896 *a Cook *b K. *t 2001.10.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Oct 11 23:02:02 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Polytene cytology of Df(3R)LK19-1 *F Polytene chromosome squashes of Df(3R)LK19-1 showed breakpoints 90E3-6;91A2-8. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0139897 *a Levis *b R. *t 2001.10.5 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Fri Oct 05 16:18:21 2001 *F To: bdgp@fruitfly.BDGP.berkeley.edu *F Subject: Apparent indel strain polymorphism within fok and neb genes *F While analyzing the flanking sequence of a P-element insertion, *F KG05435, I noticed an apparent indel strain polymorphism. According *F to the current annotation of this region in the Celera/BDGP genome *F scaffold, this polymorphism maps to within the fok gene, which is *F nested within an intron of the neb gene. *F The following shows a BLAST alignment of the KG05435 flanking *F sequence with the sequence of the Celera/BDGP scaffold segment *F AE003665.2 *F >gi|10728886|gb|AE003665.2|AE003665 Drosophila melanogaster genomic *F >scaffold 142000013386055 section 58 of *F 63, complete sequence *F Length = 157443 *F Score = 234 bits (118), Expect = 2e-60 *F Identities = 124/126 (98%) *F Strand = Plus / Minus *F Query: 122 agtccattcattcatatacacgtgccctaaacattttcccgcgactgtccacacacattc 181 *F |||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||| *F Sbjct: 86633 agtccattcattcatatacacgtgccctaaacgttttcccgcgactgtccacacacattc 86574 *F Query: 182 acacgtaggcacatgagaagtaaatataggggagacccgaagtgggcagcaaaacaagaa 241 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 86573 acacgtaggcacatgagaagtaaatataggggagacccgaagtgggcagcaaaacaagag 86514 *F Query: 242 ccgaag 247 *F |||||| *F Sbjct: 86513 ccgaag 86508 *F Score = 50.1 bits (25), Expect = 6e-05 *F Identities = 25/25 (100%) *F Strand = Plus / Minus *F Query: 1 cattaaagaatatttaaaacaaatt 25 *F ||||||||||||||||||||||||| *F Sbjct: 86660 cattaaagaatatttaaaacaaatt 86636 *F The KG05435 flank contains an insertion of ~96 nt at a position *F corresponding to ~86633 in the AE003665.2 sequence. Two other BDGP *F clone sequences, AC004364.1 and AC016445.4 also lack this insertion. *F It is my understanding that these three genome clones are all from *F the same strain background. *F While the insertion present in the KG05435 flank is lacking in these *F three BDGP genomic clones, it is present in the genomic sequence *F gi|2555104|gb|AF022650.1, which was presumably cloned from a *F different strain, and in the flanking sequence of the P-element *F insertion l(2)k07614. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0139898 *a Cherbas *b L. *t 2001.10.15 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Oct 15 18:57:32 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-tai.B}JB1 insertion. *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Lucy Cherbas (10/01). P{UAS-tai.B}JB1 is a homozygous *F viable and fertile, third chromosome insertion. The construct and its *F transformation are described in Bai et al., 2000, Cell 103:1047-1058 *F (FBrf0132259). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0139899 *a Nash *b D. *t 2001.10.15 *T personal communication to FlyBase *u *F From: William Gelbart *F To: davidnash@powersurfr.com *F Subject: A gene from your deep dark past *F Date: Fri Aug 312:27 PM *F Dear David, *F FlyBase is trying to track down the current status of genes reported *F only in an abstract. One of these is the gene E(ade)2 reported by *F Tiong and Nash in the 1989 fly meeting abstracts. *F If you could send me a note letting me know if you think we should *F keep this gene record in FlyBase, if we should merge it with some *F other gene, or if we should honorably retire it, I would appreciate *F it. Any information you have would be appreciated, and we would *F attach your reply to the record as a personal communication from you *F to FlyBase. *F Thanks in advance for any help you can provide. *F Best wishes, *F Bill *F From davidnash@powersurfr.com Mon Oct 15 13:37 EDT 2001 *F Subject: Re: A gene from your deep dark past *F From: 'David Nash' *F To: William Gelbart *F ... *F There is no doubt that there was a mutant that modified ade2 mutant *F eye-colour. It was never tested for locus specificity, so I was a bit *F uneasily with its naming. I have no doubt about the gene would, if *F identified, have something to do with purine metabolism. *F Given the way genomics is moving, it will be identified independently at *F some time, even if it has not yet been. At that time, it will be hard to *F substantiate a connection. *F I see no reason for its retention in the records, except as an aspect of *F history. *F Dave *F David Nash *F Phone: 780 435 5488 *F Address: 3512-117A Street, Edmonton, Alberta, CANADA. *F Postal Code: T6J 1V4 # *U FBrf0139900 *a Kreber *b R. *c K. *d Cook *t 2001.10.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Oct 23 15:14:22 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Df(3R)slo3 *F The following information is intended to clarify the cytology of Df(3R)slo3 *F (FBab0002926). *F According to correspondence with Bob Kreber in Barry Ganetzky's lab, U. *F Wisconsin (10/01), the aberration described in Atkinson et al., 1991 *F (Science 1991 253:551-555 (FBrf0055080)) as Df(3R)slo3 with breakpoints *F 95E6-7;96A18 is actually a synthetic deficiency formed by a large deletion *F and a smaller duplication. The deletion and duplication were isolated in *F the irradiation screen described in the paper as a four breakpoint event *F wherein the chromosomal segment deleted from chromosome 3 was split and the *F proximal portion inserted into chromosome 2. The large deletion has *F breakpoints 94D4-10;96A18. *F My own cytological analysis agreed with the previous observations. I *F observed an inverted insertional duplication with breakpoints *F 94D4-10;95E7-F1;57B3-5. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0139901 *a Kutsukake *b M. *c J. *d Hirsh *t 2001.7.19 *T personal communication to FlyBase *u *F From m-kutsukake@aist.go.jp Thu Jul 19 01:21:10 2001 *F Date: Thu, 19 Jul 2001 09:23:14 \+0900 *F To: jh6u@unix.mail.virginia.edu *F From: Mayako Kutsukake *F Subject: about hono *F Cc: jb6vz@unix.mail.virginia.edu, rd120@gen.cam.ac.uk *F Dear Jay *F I confirmed the existence of P-element of hono in the region of upstream of *F the TyrR gene. The PCR experiment I performed was as follow, *F 1. extraction of nuclear DNA from single fly of hono, excision line b2-6, *F wild type (Oregon-R). *F 2. PCR using pry4 (sense) and tyrR (antisense) primers. pry4: *F caatcatatcgctgtctcactca, tyrR: aacaccttgcacctcgtctat. The PCR condition was *F 94 degree 2', (94 degree 1', 55 degree 1', 72 degree 2'), 30 cycles, 72 *F degree 5'. The product (about 1.2 kb) was amplified from hono only. As *F positive control, PCR using tyrR primers (sense: tgatgcggtattagaagctgg, *F antisense: aacaccttgcacctcgtctat, same primer as above) also conducted and *F the product (about 900 bp) was obtained from all strain. *F 3. Sequencing the product. I found the P-lwB vector sequence and tyrR *F sequence in it. The sequence is as follows, *F TGCTGTCTCACTCAGACTCAATACGACACTCAGAATACTATTCCTTTCACTCGCACTTATTGCAAGCATACGTTAAGTG *F GATGTCTCTTGCCGACGGGACCACCTTATGTTATTTCATCATGGGCCCGGCCGAAAAGGGGAGCCGACCAAGAGGCGAA *F CTGTTGCGGGTGGCTAAAGTTTAAGGATGGACCGNTCGCCTTCAGTTCCGTGCGAATTGGCGTTGGGTGTGGACGCGAG *F TGGCGGAAGCAGGCGGCGTTGATATATACGAGCTCTTCCATCTTTCGTGATGCGGTATTAGAAGCTGGCAGCTCANAGA *F TTCCCGCAAAGTTTAAGTGACAATTTGCCAGCCAACAACAACTTTCCGACGCAGGCAACGCAAATTGAATTCGATTCGA *F TTCGATTGTCTCTCGATCTTCATCAATTCATTACGCACAGGAAAAGAGGGCGAACCGTAAAGTTNTGGTGAAAAAGTTT *F CCTGGGCTCCGTTGGCGTGGCAAAGCGACCGAAACCAAAACGAAATTTTGAAAATGAGCTTTGCTAACGACNGGCCAAA *F CCAATTAACAGAATTCGTTCTTGTGTAATAAATNAATTGCCAACAATTATAACTTGCAGTCCACTNAGGCATATTCAAA *F TGAAATGTGCCACAAAAAATGTTTACGGGTCATTGCAACTCAAAAGCGACAGACCATAGACGAGGTGCAAGGTGTTGTG *F GCAGTTGCAGAAAAACTAAAAGAAAGCCGTAAGGCTTGACCAAAAATTAATAACTGATAAAAGCAGGTAAGGCAAAACA *F CCAGCTTTAACGCGGATTTAGGATATTTAAAACATTTTATAAGAAGTGACAAGTAACTGCTATTTATTTAAAAATATAA *F TGCTTATTGCTTATTGGATGGGCGTCAAGTAGTAATAATATAGACCCACAAACTGAACAAAATTTTATTTTATGTATGT *F TTCTTCTTTGAACCTTTATCAAGTTCGGAAGCACATTACAA! *F T *F TAAAAATCAATCAATATTCTTCAAAGCATCCACTTTTATTAAAGACAGAACCTAAGAAATCAGATTTTTAAATCTCCCA *F CAAATTTTCCTTGCTCGCATTCTTCCGTAATCCCAAAAAACAAACTTCGCCCATTACCTATGCCAAGGAAAAAGGGCAA *F TCCACACTGGGAATGCCAAAAAAGAGCATGTGTGTATTAGGGAA *F I suppose that you have failed to confirm the existence of P element *F because of unsuitable primers. P-element can not be detected using your *F tyrR primers because P-element was inserted more upstream. I remember that *F I pointed out about it by phone and e-mail when you came to Japan last year. *F Therefore, I request strongly that you withdraw the appeal to the flybase. *F If you have any questions about any of this, please do not hesitate to *F contact me. *F Sincerely, *F \-- *F Mayako Kutsukake, Ph.D. *F Microbial and Genetic Resources Research Group *F Research Institute of Biological Resources *F Tsukuba Central 6 *F National Institute of Advanced Industrial Science and Technology *F 1-1-1, Higashi, Ibaraki 305-8566, Japan *F e-mail: m-kutsukake@aist.go.jp *F From rd120@gen.cam.ac.uk Mon Jul 23 15:46:19 2001 *F To: m-kutsukake@aist.go.jp, jh6u@unix.mail.virginia.edu *F Subject: Re: about hono *F Dear Mayako and Jay, *F I just looked at this using BLAST at the NCBI, out of curiosity (though *F I am a relative BLAST-novice). It seems to me that Mayako's sequence *F hits the Drosophila genome between 160103 and 161258 of genomic *F scaffold 142000013386036. *F I figured out where this hits the genome in terms of the annotations. *F The insertion site seems to be between CG7140 and TyrR, approximately *F 7kb upstream of the 5' end of CG7140, and 25kb upstream of the 5' end *F of TyrR. Since CG7140 and TyrR are divergently transcribed, the *F insertion is upstream of both of these transcription units, and it is *F not obvious to me that it is clear that the insertion site identifies *F TyrR, and not CG7140, as the gene affected in the hono mutation. It *F may be that Mayako has information that places the 5' end of TyrR much *F closer to CG7140, but if so that information isn't obvious from what we *F have in the public view of the annotations. *F In any case the insertion site described below seems very different *F from the one stated in *F \*x FBrf0126774 == Kutsukake et al., 2000, Gene 245(1): 31--42 *F where the P insertion was only 1kb or so from the 5' end of TyrR, *F so I will be leaving the FlyBase versions of the records as they are *F for now. *F Best regards, *F Rachel. *F FlyBase-Cambridge. *F From m-kutsukake@aist.go.jp Tue Jul 24 09:18:08 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: about hono *F Cc: jh6u@unix.mail.virginia.edu *F Dear Rachel *F Thank you for your reply and analysis of my sequence. I think I was short *F of explanation about my sequence. Let me describe about it more closely. *F > *F >I just looked at this using BLAST at the NCBI, out of curiosity (though *F >I am a relative BLAST-novice). It seems to me that Mayako's sequence *F >hits the Drosophila genome between 160103 and 161258 of genomic *F >scaffold 142000013386036. *F Yes. But 160193 is correct, not 160103. *F >I figured out where this hits the genome in terms of the annotations. *F >The insertion site seems to be between CG7140 and TyrR, approximately *F >7kb upstream of the 5' end of CG7140, and 25kb upstream of the 5' end *F >of TyrR. Since CG7140 and TyrR are divergently transcribed, the *F >insertion is upstream of both of these transcription units, and it is *F >not obvious to me that it is clear that the insertion site identifies *F >TyrR, and not CG7140, as the gene affected in the hono mutation. It *F >may be that Mayako has information that places the 5' end of TyrR much *F >closer to CG7140, but if so that information isn't obvious from what we *F >have in the public view of the annotations. *F Yes, I have. Information about 5'-UTR sequence of TyrR is available on the *F Genbank (Accession No. M60789; D. melanogaster octopamine receptor mRNA, *F complete cds., octopamine receptor is old name of tyramine receptor. See *F TyrR page on the flybase). These sequence was originally reported by Saudou *F et al (1990) and Arakawa et al (1990), and I also have confirmed the *F existence of this sequence by sequencing of tyrR cDNA clone that was *F screened from cDNA library. You will be able to find that TyrR 5'-UTR *F sequence (about 200 bp sequence of 5'-end of M60789) is consistent with *F 564-777th nucleotides in my sequence and with 160634-160843th nucleotides *F of genomic scaffold 142000013386036. Moreover, my TyrR cDNA (I examined 3 *F individual clones) had additional 5'-UTR that is located upstream of 200 bp *F 5'-UTR. This additional 5'-UTR was about 340 bp in length and were not *F reported anywhere, but this sequence was consistent with 160293-160633th *F nucleotides of genomic scaffold 142000013386036 and 223-563th nucleotides *F of my sequence, implying these 340 bp sequence is also 5'-UTR of TyrR. In *F my opinion based on genomic and cDNA analyses, this 5'-UTR (340 bp \+ 200 *F bp) are the first exon (but non-coding) and next first intron is about 25 *F kb. The second exon (that is thought 5'-end of TyrR on the present *F database, but I think it is not correct) is far from the first exon. I do *F not konw why the first exon is not recorded on Drosophila genome database *F (TyrR; CG7485), but I suppose that the first exon is not noticed because it *F is very far from the second exon and dose not contain coding region. If you *F agree with me, I hope that record of the database will be correct. *F The following is the result of homology search of my sequence. *F 2-123th nucleotides: identical to P-lwB vector sequence *F 223-563th nucleotides: identical to unpublished 5'-UTR of TyrR *F 564-777th nucleotides: identical to 5'-UTR of TyrR (Genbank M60789) *F In my conclusion, P-element of hono strain is inserted about 100 bp *F upstream of TryR gene. The P-element insertional effects to TyrR in hono *F mutant were described in my paper by molecular and electrophysiological *F analyses (Gene (2000) 245: 31-42) *F I will looking for your reply. *F Sincerely yours, *F \-- *F Mayako Kutsukake, Ph.D. *F Microbial and Genetic Resources Research Group *F Research Institute of Biological Resources *F Tsukuba Central 6 *F National Institute of Advanced Industrial Science and Technology *F 1-1-1, Higashi, Ibaraki 305-8566, Japan *F e-mail: m-kutsukake@aist.go.jp *F From jh6u@virginia.edu Wed Oct 24 14:20:18 2001 *F To: Mayako Kutsukake *F Subject: Re: about hono *F Cc: rd120@gen.cam.ac.uk *F Mayako- *F We recently have confirmed your localization for the hono P element. *F It took some time since we weren't able to get inverse PCR to work *F for this element. We finally used a P and flanking genomic primer. *F We were initially *F looking adjacent to the wrong exon when we failed to find it. All *F this could have been avoided if you had provided the precise location *F of the hono P when we sent you our primers sequences well over a year *F ago. In any case, I'll forward this to Rachel Drysdale & have our *F skepticism removed from flybase. It would be helpful to others if *F you could instruct Rachel to include the precise base coordinate of *F the hono P in the Flybase entry. *F I'm sorry if this has caused you any problems. *F Best regards, *F Jay # *U FBrf0141087 *a Kaminker *b J. *t 2001.11.26 *T personal communication to FlyBase *u *F From joshk@currant.lbl.gov Mon Nov 26 17:37:14 2001 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Mon, 26 Nov 2001 17:37:14 +0000 *F Date: Mon, 26 Nov 2001 09:36:56 -0800 *F From: Josh Kaminker *F User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:0.9.3) Gecko/20010808 *F X-Accept-Language: en-us *F MIME-Version: 1.0 *F To: 'Michael Ashburner (Genetics)' , suzi@bdgp.lbl.gov *F Subject: Synonyms *F Content-Transfer-Encoding: 7bit *F *F Michael, *F *F Here is the synonym list of TEs. You may have some of these already. *F *F D-element = Jockey *F Tinker = Diver *F Pilgrim = Tabor *F DMRT1A=WaldoB=Pilger *F You=IVK *F *F Josh # *U FBrf0141274 *a Mi *b H. *c P. *d Thomas *e M.D. *f Yandell *g A. *h Narechania *i M. *j Campbell *k S.E. *l Lewis *m M. *n Ashburner *o R.E. *p Foulger *t 2001.12.13 *T personal communication to FlyBase *u *F Corrections to the assignment of GO terms to various genes were made during *F the collaboration between the the Protein Informatics Group at Celera *F Genomics (H. Mi, P. Thomas, M.D. Yandell, A. Narechania, M. Campell) and *F the FlyBase group (S.E. Lewis & M. Ashburner) during the evaluation of *F the automatic assignment of GO terms to Drosophila genes by the Panther and *F LoveAtFirstSight systems, December 10-13, 2001. (Mi et al., in preparation). *F At this time the previous annotations in FlyBase were found to be in error, *F largely due non-systematic mistakes during previous annotations by FlyBase. # *U FBrf0141768 *a Roote *b J. *t 2002.1.15 *T personal communication to FlyBase *u *F >From j.roote@gen.cam.ac.uk Tue Jan 15 19:31:57 2002 *F To: rd120@mole.bio.cam.ac.uk, Aubrey de Grey *F Subject: Adhr status unknown *F *F Hello, *F *F Well the situation is probably less awful than I supposed, partly *F because of the assumptions we can make about the nBR deletions. *F *F If you just replace Adhr with Adh in all suitable places I think *F almost everything will be hunky dory, with the few exceptions where a *F ?? appears in the attached file. In those cases I suppose one could *F report osp-, Adh status unknown. (The formalised genetic data field *F would be deceptive without that caveat.) *F *F Rootles *F *F Adh status *F elA 35A4-35B1 elbow A *F *F *F *F *F L 35A4 -134.6 -- -137.8 Df(2L)A245 {35B2} - *F L 35B1 -124.9 -- -126.4 Df(2L)fn3 {35B3.4} - *F L 35B1.2 -144.4 Df(2L)A260 {35B1.2} - *F L 35B2.3 -144.4 Df(2L)A266 {35B2.3} - *F L -- Df(2L)nBR102 (see below) {--} - *F L -- -137.8 T(Y;2)TE35B-GR18[2D]R15[2P] {Y;YS;35B9-C1} - *F R 35A4 -121.8 -- -125.1 Df(2L)b84a2 {34C3} + *F R -- -137.8 T(Y;2)A80[2D]TE35B-GR18[2P] {YS;Y;35A3.4} + *F *F *F noc 35B1.2 no-ocelli *F *F *F L 35B1-3 -113.5 -- -117.5 Df(2L)A446 {35F1.2} - *F L nv -110.8 -- -113 Df(2L)A178 {nv} - *F L -- Df(2L)nBR129 {--} - *F L 35B1.2 -108 -- -114.7 In(2LR)noc4 {41} + *F L 35B1.2 -108 -- -114.7 In(2LR)noc4[L]D6[R] {35D;41;41} - *F R 35B1.2 -108 -- -114.7 In(2LR)D20[L]noc4[R] {41;41;34E4-F2} + *F L TE35B In(2L)TE35B-GR210[L]C163.41[R] {27D1.2;28B12-D1;35E1.2}- *F L TE35B In(2LR)TE35B-GR15[L]Scorv1[R] {Sco;44DE;44C3-5} - *F L TE35B In(2LR)TE35B-SR14[L]Scorv1[R] {43B3-C1;44C3-5} ?? *F L TE35B In(2LR)TE35B-SR14[L]TE35B-SZ4[R]{43B3-C1;43B3-C1} ?? *F L TE35B In(2LR)TE35B-SZ4[L]TE35B-SW250,[R], pkD {43B3-C1;43B3-C1} ?? *F L TE35B Df(2L)+(2R)pk-Drv2 {--} ?? *F L TE35B Df(2L)+(2R)pk-Drv3 {--} ?? *F L TE35B Df(2L)+(2R)pk-Drv8 {--} ?? *F L TE35B Df(2L)+(2R)pk-Drv10 {--} ?? *F L TE35B In(2LR)TE35B-SR14[L]TE35B-SW250[R], pkD{43B3-C1;43B3-C1} ?? *F L TE35B In(2LR)TE35B-SZ4[L]Scorv1[R] {43B3-C1;44C3-5} ?? *F L TE35B In(2LR)TE35B-SZ4[L]TE35B-SR14[R]{43B3-C1;43B3-C1} ?? *F L TE35B T(2;3)TE35B-GR28[2D]osp90[2P] {89B9-11;90C3-6} - *F L TE35B T(2;3)TE35B-GR28[2D]pb3[2P] {89A9.10;90C3-6} - *F L TE35B T(2;3)TE35B-GV5, Df(2L)TE35B-GV5{80-81} - *F L TE35B T(2;3)TE35B-GV4[2D]Scorv13[2P] {81;81} ?? *F L TE35B T(2;3)TE35B-GR50[2D]DTD22[2P] {35F;101;101-102} ?? *F L TE35B T(2;4)TE35B-GV101[2D]DTD22[2P] {35F;101;101-102} ?? *F L TE35B Df(2L)TE35B-GR54 {TE35B;35C1.2} - *F L TE35B Df(2L)TE35B-GW50 {34D2} - *F L TE35B Df(2L)TE35B-SR48 {--} - *F L TE35B Df(2L)TE35B-SR54 {--} - *F L TE35B Df(2L)TE35B-SW400 {--} - *F L TE35B Df(2L)TE35B-SW401 {--} - *F L TE35B Df(2L)TE35B-SW402 {--} - *F L TE35B Df(2L)TE35B-SW403 {--} - *F L/R TE35B Tp(2;3)TE35B-GV203 {34C1.2;TE35B;80-81}+ *F R TE35B Df(2L)TE35B-GV203 {31B1;34C1.2;34D2.3;76B1.2-C1.2} ?? *F R TE35B In(2LR)TE35B-GR226[L]DTD128[R] {48C6-8} ?? *F R TE35B In(2LR)DTD128[L]TE35B-GR226[R] {47B10-14} ?? *F R TE35B T(2;4)GT6[2D]TE35B-GV101[2P] {34F3;101-102} ?? *F R TE35B Df(2L)TE35B-GR300 {--} + *F R TE35B In(2LR)noc4[L]TE35B-GV50[R] {35B;41} ?? *F R TE35B In(2LR)TE35B-SR14[L]TE35B-SW250[R], pk-D{43B3-C1;43B3-C1} ?? *F R TE35B In(2LR)TE35B-SZ4[L]TE35B-SW250,[R] pk-D {43B3-C1;43B3-C1} ?? *F R TE35B In(2LR)TE35B-SR14[L]TE35B-SZ4[R]{43B3-C1;43B3-C1} ?? *F R TE35B In(2LR)TE35B-SZ4[L]TE35B-SR14[R]{43B3-C1;43B3-C1} ?? *F R TE35B T(2;3)GT10[2D]TE35B-GR209[2P] {35A1-4;76A5-7;80;80} + *F R TE35B T(2;3)H118[2D]TE35B-GV8[2P] {34C;81} ?? *F R TE35B T(2;3)H147[2D]TE35B-GV8[2P] {35B1.2;81} ?? *F R TE35B Df(2L)TE35B-SR407 {--} + *F L Sco -103 -- -106.5 T(2;3)Scorv7, noc[+/-] {Sco;35D1.2;93F11-14} - *F L Sco -103 -- -106.5 Df(2L)Scorv10, noc[+/-] {Sco;35D1.2} - *F L Sco -103 -- -106.5 Df(2L)Scorv14, noc[+/-] {Sco;35D1.2} - *F L Sco -103 -- -106.5 Df(2L)Scorv18, noc[+/-] {Sco;35D1.2} - *F L Sco -103.3 -- -107.8 In(2L)Scorv21, noc[+/-] {Sco;35D1.2;36E1.2} + *F L Sco -103 -- -106.5 Tp(2;2)Sco-1, noc[+/-] {35D1.2} + *F *F *F R nv -98 -- -103 Df(2L)TE35B-GW12 {nv} + *F L 35B1-3 -61.8 -- -66.2 In(2LR)A379, Df(2L)A379, noc[+/-] {35B1-3;41;57A8-11} - *F L 35B2.3 -61.8 -- -66.2 Df(2L)A267, noc[+/-] {35B10} - *F L 35B3 -68.3 -- -70.8 Df(2L)TE35BC-GW7, noc[+/-] {35B9-10} - *F L nv -73 Df(2L)A445, noc[+/-] {nv} - *F L 35B1.2 Df(2L)TE35D-GW20, noc[+/-] {35D4} - *F L 35B1.2 Tp(3;2)osp204, noc[+/-] {86D4.5;89A4-7} + *F L -- Df(2L)nBR119, noc[+/-] {--} - *F L nv -67.1 -- -68.3 Df(2L)osp144, noc[+/-] {nv} + *F L 35B3 -79 -- -86.6 Df(2L)nNxF1, noc[+/-] {35B10} - *F L 35B3 -73 Df(2L)nNxF2, noc[+/-] {35B10} - *F R Sco -35.5 -- -41.5 Df(2L)Scorv25, noc[+/-] {Sco} + *F *F *F L -- Df(2L)nBR127 {--} - *F L 35A4-B1 -33 -- -35.4 T(Y;2)GT1[2D]R15[2P] {Y;YS;35B9-C1} - *F L 35B2.3 0 -- -0.7 Df(2L)A48 {35D5-7} - *F L nv -13.1 -- -18.2 Df(2L)A63 {nv} - *F L nv -58 Df(2L)fn52 {nv} - *F L 35B3 T(2;3)GT7[2D]Scorv13[2P] {81;81} ?? *F R 35B1-3 In(2LR)b81a[L]DTD43[R] {34D5;41} ?? *F R [35A1.2] -75.5 -- -79 Df(2L)TE35B-GW4 {34F1.1} + *F R 35B1.2 -75.5 -- -79 Df(2L)b81a1 {34D3} + *F R 35B1.2 -37.5 -- -42.5 Df(2L)b84a4 {34D3} + *F R 35B1.2 -43.8 -- -52.8 Df(2L)b84a8 {34D3} + *F R 35B2 -75.5 -- -79 Df(2L)TE35B-GW10 {34F5} + *F R 35B2 -11.2 -- -13.3 Df(2L)GT4 {34F3} + *F R 35B3.4 Df(2L)b84a5 {34D3.4} + *F R nv Df(2L)el16 {nv} + *F R nv Df(2L)el14 {nv} + *F *F *F osp 35B1.2 outspread *F *F *F *F R [35A2.4] -11.2 -- -15.9 Df(2L)TE35B-GW8 {34E4.5} + *F R nv -8.3 Df(2L)osp144 {nv} + *F R 35B3 Tp(3;2)osp204 {35B1.2;86D4.5;89A4-7} + *F *F *F Adh 35B3 Alcohol dehydrogenase *F *F *F osp osp *F *F *F L 35B1-3 +36.9 Df(2L)osp29, osp[­] {35E6} + *F R 35B3.4 +14 -- +18 T(2;3)TE35B-GR28[2D]osp90[2P] {90C3-6;89B9-11} - *F R 35B3 >+90 T(2;3)TE35B-GR28[2D]pb3[2P] {90C3-6;83E2-8} - *F *F *F R [35A2] Df(2L)b89e68 {34D4} ?? *F R [35A4] Df(2L)el17 {34F1.2} - *F R -- Df(2L)el90 {--} - *F R -- Df(2L)nBR119 {--} - *F R -- Df(2L)nBR121 {--} - *F R -- Df(2L)nBR127 {--} - *F R 35B1.2 Df(2L)el81i1 {34F5} ?? *F R 35B1.2 >+90 Df(2L)noc10 {34F1.2} - *F R 35B1.2 >+90 Df(2L)A260 {35B1.2} - *F R 35B2 Df(2L)noc13 {35A1.2} - *F R 35B2 >+90 Df(2L)TE35B-GW15 {34F3} - *F R 35B2 >+90 Df(2L)A245 {35A4} - *F R 35B1-3 +86 In(2LR)el6[L]A379[R] {35B1-3} ?? *F R 35B1-3 +86 In(2LR)A379, Df(2L)A379 {35B1-3;41;57A8-11} - *F R 35B2.3 >+90 Df(2L)A266 {35B2.3} - *F R 35B3 +44.2 -- +51.4 Df(2L)el77 {35A1-3} - *F R 35B3 Df(2L)AdhnBR41 {34F3.4} - *F R -- Df(2L)TE35B-SW400 {TE35B} - *F R -- Df(2L)TE35B-SW401 {TE35B} - *F R -- Df(2L)TE35B-SW402 {TE35B} - *F R -- Df(2L)TE35B-SW403 {TE35B} - *F R 35B3 In(2L)75c[L]C158.1[R], Df(2L)C75RL {26D1.2;27D1.2;35A1.2} - *F R 35B4 +105 Df(2L)b81l1 {34C1} - *F R 35B4 >+90 Df(2L)fn36 {35A3} - *F R [35B5] Df(2L)osp141 {34F5} - *F R -- +51.8 -- +57.3 Df(2L)TE35B-SR48 {TE35B} - *F R -- +10 -- +30 Df(2L)TE35B-SR54 {TE35B} - *F R nv <+39.6 Df(2L)TE35B-GW11 {nv} - *F R nv Df(2L)TE35B-GW50 {nv} - *F R nv >+90 Df(2L)A63 {nv} - *F R nv >+90 Df(2L)A445 {nv} - *F R nv >+90 Df(2L)fn52 {nv} - *F R 35B2 Df(2L)PA4 {Sco;35D1.2} ?? *F R 35B2 T(2;3)Scorv7 {Sco;35D1.2;93F11-14} - *F R 35B2 Df(2L)Scorv10 {Sco;35D1.2} - *F R 35B2 Df(2L)Scorv14 {Sco;35D1.2} - *F R 35B2 Df(2L)Scorv18 {Sco;35D1.2} - *F R 35B2 +11.8 -- +13.7 Df(2L)noc20 {34F1.2} - *F R 35B1.2 +44.2 Df(2L)b84a7 {34C1} - *F R 35B2-4 Df(2L)b88c25 {34B12-C1} ?? *F R [35B10] Df(2L)b81f2A {34D3} - *F R 35B3 >+90 Df(2L)TE35B-GW3 {34F5} - *F R 35B1.2 >+90 Df(2L)b84a1 {34D3} - *F R -- Df(2L)nBR100 {--} - *F R 35B1 >+90 Df(2L)A47 {34E1} - *F L 35B3 +102 In(2L)C158.1[L]Scorv11[R] {26D1.2;24C3-9} + *F L 35B3 +102 In(2L)C158.1[L]Scorv17[R] {26D1.2;25D3-7} + *F L 35B3 Df(2L)TE35D-GW5 {35E1.2} + *F *F *F ms(2)35Bk ms(2)35Bk *F *F *F R 35B2 >+90 Df(2L)TE35B-GW7 {35A3.4} - *F R 35B2 Df(2L)fn2 {35A3.4} - *F R 35B3 >+90 Df(2L)A217 {34F5} - *F R 35B2 >+90 Df(2L)TE35B-GW9 {34F1} - *F L 35B1.3 Df(2L)TE35D-GW10 {35E1.2} + *F *F *F ms(2)35Bi ms(2)35Bi *F *F R 35B3-5 >+90 Df(2L)W {35A2.3} - *F R 35B3-5 >+90 Df(2L)fn7 *F {34E1.2} - *F L [35B5-10] >+110 T(2;3)TE35BC-GV3[2D]Scorv13[2P] {35D1.2;81;81} + *F L 35B4 >+110 Df(2L)TE35BC-GW4 {35C3} + *F *F *F *F l(2)br3 35B4 l(2)35Bb *F *F *F The following aberrations may or may not be duplicated for Adh - it is very hard to say: *F L -137.8 T(Y;2)R15[2D]TE35B-GR18[2P] {YS;Y;35B9-C1} ?? (Dp) *F R -137.8 T(Y;2)TE35B-GR18[2D]A80[2P] {Y;YS;35A3.4} ?? (Dp) *F L TE35B In(2L)C163.41[L]TE35B-GR210[R] {27D1.2;28B12-D1;35E1.2} ?? (Dp) *F L TE35B In(2LR)Scorv1[L]TE35B-GR15[R] {Sco;44DE;44C3-5} ?? (Dp) *F L TE35B In(2LR)Scorv1[L]TE35B-SR14[R] {43B3-C1;44C3-5} ?? (Dp) *F L TE35B In(2LR)Scorv1[L]TE35B-SZ4[R] {43B3-C1;44C3-5} ?? (Dp) *F L TE35B T(2;3)G40[2D]TE35B-GR28[2P] {35F4.5;91E5.6;90C3-6} ?? (Dp) *F L TE35B T(2;3)G16[2D]TE35B-GR3[2P] {35D5-7;85F1.2-86A1.2;85F6-8} ?? (Dp) *F L TE35B T(2;4)DTD22[2D]TE35B-GR50[2P] {35F;101;101-102} ?? (Dp) *F L TE35B Dp(2;2)TE35B-GR54 {35C1.2;38F} ?? (Dp) *F R TE35B Dp(2;3)TE35B-GV203 {34C1.2;TE35B;80-81}?? (Dp) *F R 35B2 [Dp(2;2)b81f1] {34D3} ?? (Dp) *F R 35B3 [Dp(2;2)Adh3] {34B1.2} ?? (Dp) *F *F *F 1 *F *F *F Also: these aberrations are Adhr status unknown: *F Df(2L)A400 *F Df(2L)ARR1 *F Df(2L)b80e3 *F Df(2L)b83d29a *F Df(2L)b88h49 *F Tp(2;3)osp[3] *F Ts(2Lt;3Lt)dpp[s19]+Ts(2Rt;3Rt)el24 *F Ts(Y;2Lt)el[4]+Ts(YSt;2Rt)A80 *F Ts(Y;2Lt)el[4]+Ts(YSt;2Rt)R15 *F Ts(Y;2Lt)GT2+Ts(YSt;2Rt)A80 *F Ts(YLt;2Lt)A80+Ts(Y;2Rt)el[4] *F Ts(YLt;2Lt)A80+Ts(YLt;2Rt)G74 *F Ts(YLt;2Lt)R15+Ts(Y;2Rt)el[4] *F Ts(YLt;2Lt)R15+Ts(YSt;2Rt)A80 *F *F *F Note: *F 1) The nBR deletions were selected on the basis of being Adh- (on 1-penten-3-ol), so we have not checked any of them *F from nBR100 onwards (BR = Baton Rouge): *F Fossett, N.G., Byrne, B.J., Kelley, S.J., Tucker, A.B., Arbour-Reily, P., Lee, W.R. *F The influence of large deletions on the mutation frequency induced by tritiated water and X-radiation in male *F Drosophila melanogaster post-meiotic germ cells. *F Mutat. Res. 1994 307(1):213--222 *F *F >From rd120 Tue Feb 19 17:21:37 2002 *F To: jr32 *F Subject: to check osp status *F *F *F here is a list-in-a-column for which you could just put osp+, *F osp-, osp? next to each. *F r *F *F *F In(2L)C163.41[L]C158[R] *F In(2LR)DTD128[L]TE35B-226[R] *F Dp(2;2)TE35B-54[L] *F Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 *F Ts(Y;2Lt)TE35B-18+Ts(YSt;2Rt)A80 *F Ts(YLt;2Lt)R15+Ts(Y;2Rt)TE35B-18 *F In(2LR)TE35B-14[L]Sco[rv1R] *F In(2LR)TE35B-4[L]TE35B-250[R] *F Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco[rv13] *F Ts(2Lt;4Lt)TE35B-50+Ts(2Rt;4Rt)DTD22 *F Df(2L)TE35B-203 *F In(2LR)noc[4L]TE35B-50[R] *F In(2LR)TE35B-14[L]TE35B-250[R] *F In(2LR)TE35B-14[L]TE35B-4[R] *F In(2LR)TE35B-4[L]TE35B-14[R] *F Ts(2Lt;3Lt)GT7+Ts(2Rt;3Rt)Sco[rv13] *F In(2LR)b81a2[L]DTD43[R] *F Ts(2Lt;4Lt)TE35B-101+Ts(2Rt;4Rt)DTD22 *F Ts(2Lt;3Lt)H118+Ts(2Rt;3Rt)TE35B-8 *F Ts(2Lt;3Lt)H147+Ts(2Rt;3Rt)TE35B-8 *F In(2LR)TE35B-4[L]Sco[rv1R] *F Ts(2Lt;3Lt)H118+Ts(2Rt;3Rt)TE35B-8 *F *F >From j.roote@gen.cam.ac.uk Fri Feb 22 08:56:05 2002 *F To: rd120@mole.bio.cam.ac.uk *F Subject: osp *F *F Dear Rachel, *F *F Thank you very much for my nice simple list. i do hope you find the *F reply equally straightforward. *F *F j *F *F In(2LR)DTD128[L]TE35B-226[R] Df osp+ *F Ts(2Lt;4Lt)GT6+Ts(2Rt;4Rt)TE35B-101 Df osp+ *F In(2LR)TE35B-14[L]Sco[rv1R] Df osp- *F In(2LR)TE35B-4[L]TE35B-250[R] neither Dp nor Df** *F Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco[rv13] Df osp+ *F Ts(2Lt;4Lt)TE35B-50+Ts(2Rt;4Rt)DTD22 Df osp- *F Df(2L)TE35B-203 Df osp+ *F In(2LR)noc[4L]TE35B-50[R] <7kb intragenic deletion within noc, osp+ *F In(2LR)TE35B-14[L]TE35B-250[R] neither Dp nor Df *F In(2LR)TE35B-14[L]TE35B-4[R] neither Dp nor Df *F In(2LR)TE35B-4[L]TE35B-14[R] neither Dp nor Df *F Ts(2Lt;3Lt)GT7+Ts(2Rt;3Rt)Sco[rv13] Df osp- *F In(2LR)b81a2[L]DTD43[R] Df osp+ *F Ts(2Lt;4Lt)TE35B-101+Ts(2Rt;4Rt)DTD22 Df osp- *F Ts(2Lt;3Lt)H118+Ts(2Rt;3Rt)TE35B-8 Df osp+ *F Ts(2Lt;3Lt)H147+Ts(2Rt;3Rt)TE35B-8 Df osp+ *F In(2LR)TE35B-4[L]Sco[rv1R] Df osp- *F Ts(2Lt;3Lt)H118+Ts(2Rt;3Rt)TE35B-8 as above Df osp+ *F *F \**neither Dp not Df. These abs are not duplicated or deleted in the *F Adh region because the breakpoints are within TE35B. # *U FBrf0141777 *a Fischbach *b K.F. *t 2001.12.10 *T personal communication to FlyBase *u *F From kff@uni-freiburg.de Mon Dec 10 11:37:07 2001 *F From: 'Prof. Dr. Karl-Friedrich Fischbach' *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (UB883) *F Dear Chihiro, *F yes UB883 is the rst-allele we mention in later papers. *F We isolated the P-element induced allele UB883 and an X-ray induced inversion, *F called 1R34 of the irreC-rst locus (no mostly called rst locus). *F UB883 is now the rst allele irreC1 *F 1R34 is now the rst allele irreC2 *F Best regards, Karl *F Chihiro Yamada schrieb: *F > Dear Dr Fischbach, *F > *F > In the course of curation, I have come across a gene in our files called, *F UB883. *F > *F > UB883 *F > UB883 *F > Maps to the right of y *F > Maps to the left of cv *F > Right limit from recombination mapping relative to cv (citation *F > unavailable) *F > Fischbach et al., 1987, J. Neurogenet. 4(2-3): 128--130 *F > Lindsley and Zimm, 1992, book *F > UB8831 *F > FBal0017337 *F > PM hybrid dysgenesis *F > Arose in: Berlin wild-type (M-cytotype) females x Harwich *F > (P-cytotype) males. *F > J. Campos-Ortega. *F > Eye mutant. Inner optic chiasma in disorder. Bundles of fibers *F > connecting medulla and lobula plate penetrate neuropil of *F > lobula. *F > *F > I suspect very strongly that this is the same as the allele called *F > irreCUB883 in your later papers. Can you confirm this to be the *F > case? *F > *F > Secondly, At the moment we have an allele rstirreC1, which is said to *F > be the same allele as irreCUB883. I have suspicions that this is not *F > the case, but in fact irreC1 and UB883 are two independant alleles, *F > however, the first paper in which our curation makes the link between *F > these two alleles, is not easily available to me. *F > *F > Boschert et al., 1990, J. Neurogenet. 6: 153--171 *F > *F > Can you confirm one way or another the identity of these alleles? *F > *F > It would be great if you could answer these questions for me, as I *F > could then make a personal communication from you to FlyBase to which I *F > could attribute this clean up of these genes and alleles. *F > *F > Thanks *F > *F > Chihiro # *U FBrf0141780 *a Robertson *b H. *t 2001.11.11 *T personal communication to FlyBase *u *F Date: Sun, 11 Nov 2001 22:23:22 \-0600 *F From: Hugh Robertson *F Subject: Re: annotation revisions *F To: Gillian Millburn , *F Whitfield_Eleanor *F Dear Gillian and Eleanor, *F Attached is a FASTA file of the Drosophila gustatory receptor proteins with *F their agreed names, largely following the recent Current Biology paper from *F Hubert Amrein's group. (names with a period and letter after them are various *F alternatively spliced products that are largely different proteins, but share *F their C-termini). *F .. *F Hugh *F .. *F Hugh M. Robertson, Professor *F Department of Entomology, University of Illinois at Urbana-Champaign *F 320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801 *F Phone 217-333-0489; FAX 217-244-3499; email hughrobe@uiuc.edu *F WWW http://www.life.uiuc.edu/robertson/lab.html *F \------------------------------------------------------------------------------ *F -- *F >Gr2a *F MEFGMDTLRALEPLHRACQVCNLWPWRLAPPPDSEGILLRRSRWLELYGWTVLIAATSFTVYGLFQESSVEEKQDSEST *F ISSIGHTVDFIQLVGMRVAHLAALLEALWQRQAQRGFFAELGEIDRLLSKALRVDVEAMRINMRRQTSRRAVWILWGYA *F VSQLLILGAKLLSRGDRFPIYWISYLLPLLVCGLRYFQIFNATQLVRQRLDVLLVALQQLQLHQKGPAVDTVLEEQEDL *F EEAAMDRLIAVRLVYQRVWALVALLNRCYGLSMLMQVGNDFLAITSNCYWMFLNFRQSAASPFDILQIVASGVWSAPHL *F GNVLVLSLLCDRTAQCASRLALCLHQVSVDLRNESHNALITQFSLQLLHQRLHFSAAGFFNVDCTLLYTIVGATTTYLI *F ILIQFHMSESTIGSDSNGQ *F >Gr5a *F MSTFILITFYNPESQVRGTRKNFLHDGSFHEAVAPVLAVAQCFCLMPVCGISAPTYRGLSFNRRSWRFWYSSLYLCSTS *F VDLAFSIRRVAHSVLDVRSVEPIVFHVSILIASWQFLNLAQLWPGLMRHWAAVERRLPGYTCCLQRARPARRLKLVAFV *F LLVVSLMEHLLSIISVVYYDFCPRRSDPVESYLLGASAQLFEVFPYSNWLAWLGKIQNVLLTFGWSYMDIFLMMLGMGL *F SEMLARLNRSLEQQVRQPMPEAYWTWSRTLYRSIVELIREVDDAVSGIMLISFGSNLYFICLQLLKSINTMPSSAHAVY *F FYFSLLFLLSRSTAVLLFVSAINDQAREPLRLLRLVPLKGYHPEVFRFAAELASDQVALTGLKFFNVTRKLFLAMAGTV *F ATYELVLIQFHEDKKTWDCSPFNLD *F >Gr8a *F MSGHLGRVLQFHLRLYQVLGFHGLPLPGDGNPARTRRRLMAWSLFLLISLSALVLACLFSGEEFLYRGDMFGCANDALK *F YVFAELGVLAIYLETLSSQRHLANFWWLHFKLGGQKTGLVSLRSEFQQFCRYLIFLYAMMAAEVAIHLGLWQFQALTQH *F MLLFWSTYEPLVWLTYLRNLQFVLHLELLREQLTGLEREMGLLAEYSRFASETGRSFPGFESFLRRRLVQKQRIYSHVY *F DMLKCFQGAFNFSILAVLLTINIRIAVDCYFMYYSIYNNVINNDYYLIVPALLEIPAFIYASQSCMVVVPRIAHQLHNI *F VTDSGCCSCPDLSLQIQNFSLQLLHQPIRIDCLGLTILDCSLLTRMACSVGTYMIYSIQFIPKFSNTYM *F >Gr10a *F MTSPDERKSFWERHEFKFYRYGHVYALIYGQVVIDYVPQRALKRGVKVLLIAYGHLFSMLLIVVLPGYFCYHFRTLTDT *F LDRRLQLLFYVSFTNTAIKYATVIVTYVANTVHFEAINQRCTMQRTHLEFEFKNAPQEPKRPFEFFMYFKFCLINLMMM *F IQVCGIFAQYGEVGKGSVSQVRVHFAIYAFVLWNYTENMADYCYFINGSVLKYYRQFNLQLGSLRDEMDGLRPGGMLLH *F HCCELSDRLEELRRRCREIHDLQRESFRMHQFQLIGLMLSTLINNLTNFYTLFHMLAKQSLEEVSYPVVVGSVYATGFY *F IDTYIVALINEHIKLELEAVALTMRRFAEPREMDERLTREIEHLSLELLNYQPPMLCGLLHLDRRLVYLIAVTAFSYFI *F TLVQFDLYLRKKS *F >Gr10b *F MRVGKLCRLALRFWMGLILVLGFSSHYYNPTRRRLVYSRILQTYDWLLMVINLGAFYLYYRYAMTYFLEGMFRRQGFVN *F QVSTCNVFQQLLMAVTGTWLHFLFERHVCQTYNELSRILKHDLKLKEHSRFYCLAFLAKVYNFFHNFNFALSAIMHWGL *F RPFNVWDLLANLYFVYNSLARDAILVAYVLLLLNLSEALRLNGQQEHDTYSDLMKQLRRRERLLRIGRRVHRMFAWLVA *F IALIYLVFFNTATIYLGYTMFIQKHDALGLRGRGLKMLLTVVSFLVILWDVVLLQVICEKLLAEENKICDCPEDVASSR *F TTYRQWEMSALRRAITRSSPENNVLGMFRMDMRCAFALISCSLSYGIIIIQIGYIPG *F >Gr21a *F MSFWAVSRGLTPPSKVVPMLNPNQRQFLEDEVRYREKLKLMARGDAMEEVYVRKQETVDDPLELDKHDSFYQTTKSLLV *F LFQIMGVMPIHRNPPEKNLPRTGYSWGSKQVMWAIFIYSCQTTIVVLVLRERVKKFVTSPDKRFDEAIYNVIFISLLFT *F NFLLPVASWRHGPQVAIFKNMWTNYQYKFFKTTGSPIVFPNLYPLTWSLCVFSWLLSIAINLSQYFLQPDFRLWYTFAY *F YPIIAMLNCFCSLWYINCNAFGTASRALSDALQTTIRGEKPAQKLTEYRHLWVDLSHMMQQLGRAYSNMYGMYCLVIFF *F TTIIATYGSISEIIDHGATYKEVGLFVIVFYCMGLLYIICNEAHYASRKVGLDFQTKLLNINLTAVDAATQKEVEMLLV *F AINKNPPIMNLDGYANINRELITTNISFMATYLVVLLQFKITEQRRIGQQQA *F >Gr22a *F MSQPKRIHRICKGLARFTIRATLYGSWVLGLFPFTFDSRKRRLNRSKWLLAYGLVLNLTLLVLSMLPSTDDHNSVKVEV *F FQRNPLVKQVEELVEVISLITTLVTHLRTFSRSSELVEILNELLVLDKNHFSKLMLSECHTFNRYVIEKGLVIILEIGS *F SLVLYFGIPNSKIVVYEAVCIYIVQLEVLMVVMHFHLAVIYIYRYLWIINGQLLDMASRLRRGDSVDPDRIQLLLWLYS *F RLLDLNHRLTAIYDIQVTLFMATLFSVNIIVGHVLVICWINITRFSLLVIFLLFPQALIINFWDLWQGIAFCDLAESTG *F KKTSMILKLFNDMENMDQETERRVAEFTLFCSHRRLKVCHLGLLDINYEMGFRMIITNILYVVFLVQFDYMNLKFKTD *F >Gr22b *F MFGSSREIRPYLARQMLKTTLYGSWLLGIFPFTLDSGKRIRQLRRSRCLTLYGLVLNYFLIFTLIRLAFEYRKHKLEAF *F KRNPVLEMINVVIGIINVLSALIVHFMNFWGSRKVGEICNELLILEYQDFEGLNGRNCPNFNCFVIQKCLTILGQLLSF *F FTLNFALPGLEFHICLVLLSCLMEFSLNLNIMHYHVGVLLIYRYVWLINEQLKDLVSQLKLNPETDFSRIHQFLSLYKR *F LLELNRKLVIAYEYQMTLFIIAQLSGNIVVIYFLIVYGLSMRTYSIFLVAFPNSLLINIWDFWLCIAACDLTEKAGDET *F AIILKIFSDLEHRDDKLEMSVNEFAWLCSHRKFRFQLCGLFSMNCRMGFKMIITTFLYLVYLVQFDYMNL *F >Gr22c *F MFASRSDLQSRLCWIILKATLYSSWFLGVFPYRFDSRNGQLKRSRFLLFYGLILNFFLLLKMVCSGGQKLGIPEAFARN *F SVLENTHYTTGMLAVFSCVVIHFLNFWGSTRVQDLANELLVLEYQQFASLNETKCPKFNSFVIQKWLSVIGLLLSYLSI *F AYGLPGNNFSVEMVLINSLVQFSFNCNIMHYYIGVLLIYRYLWLINGQLLEMVTNLKLDCSVDSSRIRKYLSLYRRLLE *F LKGYMVATYEYHMTLVLTTGLASNFLAIYSWIVLDISMNINFIYLLIFPLFLLVNVWNLWLSIAASDLAENAGKSTQTV *F LKLFADLEVKDIELERSVNEFALLCGHCQFNFHVCGLFTINYKMGFQMIITSFLYLIYMIQFDFMNL *F >Gr22d *F MFRPRCGLRQKFVYVILKSILYSSWLLGIFPFKYEPKKRRLRRSMWLIPFGVVISSSLLILMVKQSAEDREHGIMLDVF *F QRNALLYQISSLMGVVGVVSICTVHLRTLWRSKHLEEIYNGLMLLEAKYFCSNAVECPAFDGYVIQKGVVIVVGLLAPW *F MVHFGMPDSKLPVLNVLVVSMVKLGTLLLALHYHLGVVIIYRFVWLINRELLSLVCSLRGNHKGSSSRVRFLLKLYNKL *F VNLYSKLADCYDCQTVLMMAIFLAANIIVCFYMIVYRISLSKMSFFVMLIMFPLAIANNFMDFWLSMKVCDLLQKTGRQ *F TSMILKLFNDIENMDKDLEISISDFALYCSHRRFKFLHCGLFHVNREMGFKMFVASVLYLLYLVQFDYMNL *F >Gr22e *F MFRPSGSGYRQKWTGLTLKGALYGSWILGVFPFAYDSWTRTLRRSKWLIAYGFVLNAAFILLVVTNDTESETPLRMEVF *F HRNALAEQINGIHDIQSLSMVSIMLLRSFWKSGDIERTLNELEDLQHRYFRNYSLEECISFDRFVLYKGFSVVLELVSM *F LVLELGMSPNYSAQFFIGLGSLCLMLLAVLLGASHFHLAVVFVYRYVWIVNRELLKLVNKMAIGETVESERMDLLLYLY *F HRLLDLGQRLASIYDYQMVMVMVSFLIANVLGIYFFIIYSISLNKSLDFKILVFVQALVINMLDFWLNVEICELAERTG *F RQTSTILKLFNDIENIDEKLERSITDFALFCSHRRLRFHHCGLFYVNYEMGFRMAITSFLYLLFLIQFDYWNL *F >Gr22f *F MKMFQPRRGFSCHLAWFMLQTTLYASWLLGLFPFTFDSRRKQLKRSRWLLLYGFVLHSLAMCLAMSSHLASKQRRKYNA *F FERNPLLEKIYMQFQVTTFFTISVLLLMNVWKSNTVRKIANELLTLEGQVKDLLTLKNCPNFNCFVIKKHVAAIGQFVI *F SIYFCLCQENSYPKILKILCCLPSVGLQLIIMHFHTEIILVYRYVWLVNETLEDSHHLSSSRIHALASLYDRLLKLSEL *F VVACNDLQLILMLIIYLIGNTVQIFFLIVLGVSMNKRYIYLVASPQLIINFWDFWLNIVVCDLAGKCGDQTSKVLKLFT *F DLEHDDEELERSLNEFAWLCTHRKFRFQLCGLFSINHNMGFQMIITSFLYLVYLLQFDFMNL *F >Gr23a.a *F MVMVQSVSVYKANMKTLECLTRRFLEVIFSVLALVPLPPISQLGWLFLSLAIRCCWIVYFIYLLDVAISFSWVAIENVG *F NAVGTMLFVGNSVLGFALLLESVLKQKTHSQLEDLRVQTELQLQRLGMFGRSRHAAYLLPLIGVQFTCDLVRLATNFGE *F TVSPVFCISLPLMWLLRYRYVQLVQHVMDLNQRSIHLRRSLLSMASGNDLWQPYGVQECLQLQTLRTTYERIFECYETF *F SDCYGWGMLGLHLLTSFQFVTNAYWMIMGIYDGGNVRSLIFNGATGIDFGTPIATLFWHGDSGAENGRQIGCLISKLVK *F PQGSKLYNDLVSEFSLQTLHQRFVVTAKDFFSLNLHLLSSMFAAVVTYLVILIQFMFAERSSTRGSG *F >Gr23a.b *F MFPPTRVQASSRVVLKIFHFILVAFSLRSRRLSRLVLWLQFLGWLTWFISMWTQSVIYAQTIDCTLDCSLRHILTFFQT *F VSHAFIVVTSFLDGFRIKQDQLDEPIAFEDSDPWLAFTVLAMLVPTLGVEYLVCSNAPEYAFRIRIYHLKTLPSFLALQ *F VQIISFILEVMKVNIRVRQTKLQLLILARELSCRWPQRKQKPQFSDQQAHRVKDLKRRYNDLHYLFVRINGYFGGSLLT *F IIIVHFAIFVSNSYWLFVDIRTRPWRIYAILLNLGFIFNVALQMAAACWHCQQSYNLGRQIGCLISKLVKPQGSKLYND *F LVSEFSLQTLHQRFVVTAKDFFSLNLHLLSSMFAAVVTYLVILIQFMFAERSSTRGSG *F >Gr28a *F MAFKLWERFSQADNVFQALRPLTFISLLGLAPFRLNLNPRKEVQTSKFSFFAGIVHFLFFVLCFGISVKEGDSIIGYFF *F QTNITRFSDGTLRLTGILAMSTIFGFAMFKRQRLVSIIQNNIVVDEIFVRLGMKLDYRRILLSSFLISLGMLLFNVIYL *F CVSYSLLVSATISPSFVTFTTFALPHINISLMVFKFLCTTDLARSRFSMLNEILQDILDAHIEQLSALELSPMHSVVNH *F RRYSHRLRNLISTPMKRYSVTSVIRLNPEYAIKQVSNIHNLLCDICQTIEEYFTYPLLGIIAISFLFILFDDFYILEAI *F LNPKRLDVFEADEFFAFFLMQLIWYIVIIVLIVEGSSRTILHSSYTAAIVHKILNITDDPELRDRLFRLSLQLSHRKVL *F FTAAGLFRLDRTLIFTITGAATCYLIILIQFRFTHHMDDTSSNSTNNLHSIHLGD *F >Gr28b.a *F MIRCGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSWYGFLNAIFRWLLLAYCYSY *F INLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPLLLRKYFLKSVKNMVQVDTQLERLRSPVNFNTVVGQVVL *F VILAVVLLDTVLLTTGLVCLAKMEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRITCLHKVLKNLAHQWDTRSL *F KAVNQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLG *F KSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINF *F TAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28b.b *F MSALRRVRKYFISSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNIFIYICLCGFCYISSLRQGESIV *F GYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFSNIGVRVKYSRIFRYSLLVLIFKLLIL *F GVYFVGVFRLLVSLDVTPSFCVCMTFFLQHSVVSIAICLFCVIAFSFERRLSIINQVLKNLAHQWDTRSLKAVNQKQRS *F LQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFK *F TVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGLFNID *F RTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28b.c *F MDIEMAKEPVNPTDTPDIEVTPGLCQPLRRRFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGKKAIKKTIFG *F YINGIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIGVTVIYLTAFVPNHRLERCLQKFHTMDVQL *F QTVGVKIMYSKVLRFSYMVLISMFLVNVLFTGGTFSVLYSSEVAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLV *F MVNKVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISI *F AFLIIVFDAYYVLETLLGKSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEV *F KEKLQQFSMQLMHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28b.d *F MSFYFCEIFKPRDAFGAEQTLLLYTYLLGLTPFRLRGQAGERQFHLSKIGYLNAFLQLSFFSYCFLAALIEQQSIVGYF *F FKSEISQMGDSLQKFIGMTGMSILFLCSSIRVRLLIHIWDRISYIDDRFLNLGVCFNYPAIMRLRLLQIFLINGVQLGY *F LISSNWMLLGNDVRPIYTAIVAFYVPQIFLLSIVMLFNATLHRLWQHFTVLNQVLKNLAHQWDTRSLKAVNQKQRSLQC *F LDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVE *F FVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGLFNIDRTL *F YFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28b.e *F MWLLRRSVGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINIIIRMAIYMVNSFYGAVNRDT *F LMSNFFLTDISNVIDALQKINGMLGIFAILLISLLNRKELLKLLATFDRLETEAFPRVGVAMHQVAANKKMNRLVIILV *F GSMVAYITCSFLMISLRDTTTFSISAVISFFSPHFIVCAVSFLAGNVMIKLRIYLSALNEVLKNLAHQWDTRSLKAVNQ *F KQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRE *F SKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGL *F FNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr32a *F MPARNHDHPVFEDIRTILSVLKASGLMPIYEQVSDYEVGPPTKTNEFYSFFVRGVVHALTIFNVYSLFTPISAQLFFSY *F RETDNVNQWIELLLCILTYTLTVFVCAHNTTSMLRIMNEILQLDEEVRRQFGANLSQNFGFLVKFLVGITACQAYIIVL *F KIYAVQGEITPTSYILLAFYGIQNGLTATYIVFASALLRIVYIRFHFINQLLNGYTYGQQHRRKEGGARARRQRGDVNP *F NVNPALMEHFPEDSLFIYRMHNKLLRIYKGINDCCNLILVSFLGYSFYTVTTNCYNLFVQITGKGMVSPNILQWCFAWL *F CLHVSLLALLSRSCGLTTTEANATSQILARVYAKSKEYQNIIDKFLTKSIKQEVQFTAYGFFAIDNSTLFKIFSAVTTY *F LVILIQFKQLEDSKVEDPVPEQT *F >Gr33a *F MIQIMNWFSMVKIKKMLVIGLIPLNRQQSETNFILDYAMMCIVPIFYVACYLLINLSHIIGLCLLDSCNSVCKLSSHLF *F MHLGAFLYLTITLLSLYRRKEFFQQFDARLNDIDAVIQKCQRVAEMDKVKVTAVKHSVAYHFTWLFLFCVFTFALYYDV *F RSLYLTFGNLAFIPFMVSSFPYLAGSIIQGEFIYHVSVISQRFEQINMLLEKINQEARHRHAPLTVFDIESEGKKERKT *F VTPITVMDGRTTTGFGNENKFAGEMKRQEGQQKNDDDDLDTSNDEDEDDFDYDNATIAENTGNTSEANLPDLFKLHDKI *F LALSVITNGEFGPQCVPYMAACFVVSIFGIFLETKVNFIVGGKSRLLDYMTYLYVIWSFTTMMVAYIVLRLCCNANNHS *F KQSAMIVHEIMQKKPAFMLSNDLFYNKMKSFTLQFLHWEGFFQFNGVGLFALDYTFIFSTVSAATSYLIVLLQFDMTAI *F LRNEGLMS *F >Gr36a *F MFDWVGLLLKVLYYYGQIIGLINFEIDWQRGRVVAAQRGILFAIAINVLICMVLLLQISKKFNLDVYFGRANQLHQYVI *F IVMVSLRMASGISAILNRWRQRAQLMRLVECVLRLFLKKPHVKQMSRWAILVKFSVGVVSNFLQMAISMESLDRLGFNE *F FVGMASDFWMSAIINMAISQHYLVILFVRAYYHLLKTEVRQAIHESQMLSEIYPRRAAFMTKCCYLADRIDNIAKLQNQ *F LQSIVTQLNQVFGIQGIMVYGGYYIFSVATTYITYSLAINGIEELHLSVRAAALVFSWFLFYYTSAILNLFVMLKLFDD *F HKEMERILEERTLFTSALDVRLEQSFESIQLQLIRNPLKIEVLDIFTITRSSSAAMIGSIITNSIFLIQYDMEYF *F >Gr36b *F MVDWVVLLLKAVHIYCYLIGLSNFEFDCRTGRVFKSRRCTIYAFMANIFILITIIYNFTAHGDTNLLFQSANKLHEYVI *F IIMSGLKIVAGLITVLNRWLQRGQMMQLVKDVIRLYMINPQLKSMIRWGILLKAFISFAIELLQVTLSVDALDRQGTAE *F MMGLLVKLCVSFIMNLAISQHFLVILLIRAQYRIMNAKLRMVIEESRRLSFLQLRNGAFMTRCCYLSDQLEDIGEVQSQ *F LQSMVGQLDEVFGMQGLMAYSEYYLSIVGTSYMSYSIYKYGPHNLKLSAKTSIIVCILITLFYLDALVNCNNMLRVLDH *F HKDFLGLLEERTVFASSLDIRLEESFESLQLQLARNPLKINVMGMFPITRGSTAAMCASVIVNSIFLIQFDMEFF *F >Gr36c *F MDLESFLLGAVYYYGLFIGLSNFEFDWNTGRVFTKKWSTLYAIALDSCIFALYIYHWTGNTNIVNAIFGRANMLHEYVV *F AILTGLRIVTGLFTLILRWYQRCKMMDLASKVVRMYVARPQVRRMSRWGILTKFIFGSITDGLQMAMVLSAMGSVDSQF *F YLGLGLQYWMFVILNMAMMQQHMIMLFVRTQFQLINTELRQVIDEAKDLLLSPRHQGVFMTKCCSLADQIENIARIQSQ *F LQTIMNQMEEVFGIQGAMTYGGYYLSSVGTCYLAYSILKHGYENLSMTLSTVILAYSWCFFYYLDGMLNLSVMLHVQDD *F YWEMLQILGKRTIFVGLDVRLEEAFENLNLQLIRNPLKITVVKLYDVTRSNTMAMFGNLITHSIFLIQYDIEHF *F >Gr36d *F MPEKKGHEIPLRNSRKKWLSHLLRWSVSSICWISYIIYRGVVVGQMKFDPRNRKMIIHPRNIWTKRISLLLKILAMLWD *F YYFIQYLCSAILPIFSNKSERFFNNLIEAIAVQLSLWNTARLYSWLNSLSWNRSFVDRVNDVIRLNGHLKSILGPLSLD *F SISLLILYVVHLQFTVLQTINHPYMIPMVNTLLLGLICNVYVAYQMLLLSWIAAINNFLKYSQPEQQPNRKQRKNLLRL *F LRIYAKISNVHQDIKVLWLPVASMLFSNIVELVRNWSYIIEWIFFHRRKTVMQKWSFIFWKYLGGGLAPLLRMLLIGLC *F NDRLVQMQDFLNLQLLIIDLRHTKFKQLDGNFVSQFKSLQTCFDLQLRAQPIRNQIMSLNQECGCPFALDFFFCTVLNS *F ISCVQYKMANEINND *F >Gr39a.a *F MGTRNRKLLFFLHYQRYLGLTNLDFSKSLHIYWLHGTWSSTAIQIVVVGVFMAALLGALAESLYYMETKSQTGNTFDNA *F VILTTSVTQLLANLWLRSQQKSQVNLLQRLSQVVELLQFEPYAVPQFRWLYRIWLLVCLIYGAMVTHFGINWLTTMQIS *F RVLTLIGFVYRCVLANFQFTCYTGMVVILKKLLQVQVKQLEHLVSTTTISMAGVAGCLRTHDEILLLGQRELIAVYGGV *F ILFLFIYQVMQCILIFYISNLEGFHSSNDLVLIFCWLAPMLFYLILPLVVNDIHNQANKTAKMLTKVPRTGTGLDRMIE *F KFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F >Gr39a.b *F MDFQPGELCAYYRLCRYLGIFCIDYNPTKKKFRLRRSVLCYIVHFALQAYLVGCISVMVTYWRRCFKSELTTTGNHFDR *F LVMVIALGILVVQNAWLIWLQAPHLRIVRQIEFYRRNHLANVRLLLPKRLLWLIIATNVVYMANFIKTCIFEWLTDASR *F LFVITSLGFPLRYLVTSFTMGTYFCMVHIVRLVLDWNQSQINAIIDESADLKMTSPNRLRLRVCLEMHDRLMLLCNDEI *F SLVYGFIAWLSWMFASLDVTGVIYLTMVIQTKKSIVLKLITNVVWLSPTFMTCAASFMSNRVTIQANKTAKMLTKVPRT *F GTGLDRMIEKFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F >Gr39a.c *F MKRNAFEELRVQLRTLKWLGVLRFTIDFNKCLVRENASEERSAWLYLIGVVGITCSLIVYSTYFPSHFIMGKHNTTGNC *F YALINIRSCSIVTMLIYTQLYIQRFRFVALLQSILRFNQISGSHREEGRFAFYYYTHLSLLIICMLNYAYGYWTAGVRL *F TTIPIYLLQYGFSYLFLGQVVVLFACIQQILLSILKYYNQVVLKNIKSSKESREFYYNFCKYNQVIWLSYTEINHCFGL *F LLLLVTGLILLITPSGPFYLVSTIFEGRFRQNWQFSLMSFTAILWSLPWIVLLVLAMGRNDVQKEANKTAKMLTKVPRT *F GTGLDRMIEKFLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F >Gr39a.d *F MSKVCRDLRIYLRLLHIMGMMCWHFDSDHCQLVATSGSERYAVVYAGCILVSTTAGFIFALLHPSRFHIAIYNQTGNFY *F EAVIFRSTCVVLFLVYVILYAWRHRYRDLVQHILRLNRRCASSCTNQQFLHNIILYGMLTILCFGNYLHGYTRAGLATL *F PLALCMLVYIFAFLVLCLLLMFFVSLKQVMTAGLIHYNQQLCQGDLISGLRGRQQILKLCGGELNECFGLLMLPIVALV *F LLMAPSGPFFLISTVLEGKFRPDECLIMLLTSSTWDTPWMIMLVLMLRTNGISEEANKTAKMLTKVPRTGTGLDRMIEK *F FLLKNLRQKPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F >Gr39b *F MLYSFHPYLKYFALLGLVPWSESCAQSKFVQKVYSAILIILNAVHFGISIYFPQSAELFLSLMVNVIVFVARIVCVTVI *F ILQVMVHYDDYFRFCREMKYLGLRLQCELKIHVGRLKWQSYAKILALGIGFLVTVLPSIYVALSGSLLYFWSSLLSILI *F IRMQFVLVLLNVELLGHHVSLLGIRLQNVLECHLMGANCTLDGNANRLCSLEFLLALKQSHMQLHYLFTHFNDLFGWSI *F LGTYVVLFSDSTVNIYWTQQVLVEVYEYKYLYATFSVFVPSFFNILVFCRCGEFCQRQSVLIGSYLRNLSCHPSIGRET *F SYKDLLMEFILQVEQNVLAINAEGFMSTDNSLLMSILAAKVTYLIVLMQFSSV *F >Gr43a *F MEISQPSIGIFYISKVLALAPYATVRNSKGRVEIGRSWLFTVYSATLTVVMVFLTYRGLLFDANSEIPVRMKSATSKVV *F TALDVSVVVMAIVSGVYCGLFSLNDTLELNDRLNKIDNTLNAYNNFRRDRWRALGMAAVSLLAISILVGLDVGTWMRIA *F QDMNIAQSDTELNVHWYIPFYSLYFILTGLQVNIANTAYGLGRRFGRLNRMLSSSFLAENNATSAIKPQKVSTVKNVSV *F NRPAMPSALHASLTKLNGETLPSEAAGDKAAARSLILNVELLKLGYFPAKNKGLLLKSLADSHESLGKCVHLLSNSFGI *F AVLFILVSCLLHLVATAYFLFLELLSKRDNGYLWVQMLWICFHFLRLLMVVEPCHLAARESRKTIQIVCEIERKVHEPI *F LAEAVKKFWQQLLVVDADFSACGLCRVNRTILTSFASAIATYLVILIQFQRTNG *F >Gr43b *F MSTGSHSPEAMWSATNFRRHQRKPNQVLHRWFFKGSAWIIYAIACGLHFFKLHYNERTNQVEESQYHRIWSKIVVVLKV *F ILLASPYLQYFVLGLGIYIHITLVQDSKAQNFLMSLIVLGIVIGVLRRLLIFLHLKRDRRFLKHTVNEILHITSALEQK *F FGMEYKCDSTLLVVYLAKLWILTVMLDSLWYKPYFLSSIFLYWVLLEYCFAGYFIYQLILLSWYHTIILFLQRFIEDHA *F NRLDIELHYHRRLIPLFELHLRINNLHKHVRDNVSWLSTSVYLMIFTCIFNAELLIECSLFAGDELENKIYIITDGCLG *F PVCVPILYVLILGMCTDRFRDAEVQLQQLFVIVQGLYMRKVRPHLLIAMVLENEHTSLIIHQKLKPLENMIILDITCDR *F EFVMDYIVTVILTALSLVQYTISTGGNISECVTHK *F >Gr47a *F MAFTSSQLCSLLTKFTALNGLNTYYFDTKTNAFRVSSKLKIYCAIHHALCVLALAHMSYSTASNLRVSVTVLTIGGTMA *F CCVKSCWEKAQGIRNLARGLVTMEQKYFAGRPSGLLLKCRYYIKITFGSITLLRIHLIQPIYMRRLLPSQFYLNVGAYW *F LLYNMLLAAVLGFYFLLWEMCRIQKLINDQMTLILARSGQRNRLKKMQHCLRLYSKLLLLCDQFNSQLGHVAIWVLACK *F SWCQITFGYEIFQMVAAPKSIDLTMSMRVFVIFTYIFDAMNLFLGTDISELFSTFRADSQRILRETSRLDRLLSMFALK *F LALHPKRVVLLNVFTFDRKLTLTLLAKSTLYTICCLQNDYNKLKA *F >Gr47b *F MQRDDGFVYCYGNLYSLLLYWGLVTIRVRSPDRGGAFSNRWTVCYALFTRSFMVICFMATVMTKLRDPEMSAAMFGHLS *F PLVKAIFTWECLSCSVTYIEYCLSLDLQKDRHLKLVARMQEFDRSVLMVFPHVQWNYRRARLKYWYGTVIVGFCFFSFS *F ISLIFDTTRCTCGIPSTLLMAFTYTLLTSSVGLLGFVHIGIMDFIRVRLRLVQQLLHQLYQADDSSEVHERIAYLFEMS *F KRCSFLLAELNGVFGFAAAAGIFYDFTIMTCFVYVICQKLLEREPWDPEYVYMLLHVAIHTYKVVITSTYGYLLLRE *F >Gr57a *F MAVLYFFREPETVFDCAAFICILQFLMGCNGFGIRRSTFRISWASRIYSMSVAIAAFCCLFGSLSVLLAEEDIRERLAK *F ADNLVLSISALELLMSTLVFGVTVISLQVFARRHLGIYQRLAALDARLMSDFGANLNYRKMLRKNIAVLGIVTTIYLMA *F INSAAVQVASGHRALFLLFALCYTIVTGGPHFTGYVHMTLAEMLGIRFRLLQQLLQPEFLNWRFPQLHVQELRIRQVVS *F MIQELHYLIQEINRVYALSLWAAMAHDLAMSTSELYILFGQSVGIGQQNEEENGSCYRMLGYLALVMIPPLYKLLIAPF *F YCDRTIYEARRCLRLVEKLDDWFPQKSSLRPLVESLMSWRIQAKIQFTSGLDVVLSRKVIGLFTSILVNYLLILIQFAM *F TQKMGEQIEQQKIALQEWIGF *F >Gr58a *F MLLKFMYIYGIGCGLMPAPLKKGQFLLGYKQRWYLIYTACLHGGLLTVLPFTFPHYMYDDSYMSSNPVLKWTFNLTNIT *F RIMAMFSGVLLMWFRRKRILNLGENLILHCLKCKTLDNRSKKYSKLRKRVRNVLFQMLLVANLSILLGALILFRIHSVQ *F RISKTAMIVAHITQFIYVVFMMTGICVILLVLHWQSERLQIALKDLCSFLNHEERNSLTLSENKANRSLGKLAKLFKLF *F AENQRLVREVFRTFDLPIALLLLKMFVTNVNLVYHGVQFGNDTIETSSYTRIVGQWVVISHYWSAVLLMNVVDDVTRRS *F DLKMGDLLREFSHLELVKRDFHLQLELFSDHLRCHPSTYKVCGLFIFNKQTSLAYFFYVLVQVLVLVQFDLKNKVEKRN *F >Gr58b *F MLHPKLGRVMNVVYYHSVVFALMSTTLRIRSCRKCLRLEKVSRTYTIYSFFVGIFLFLNLYFMVPRIMEDGYMKYNIVL *F QWNFFVMLFLRAIAVVSCYGTLWLKRHKIIQLYKYSLIYWKRFGHITRAIVDKKELLDLQESLARIMIRKIILLYSAFL *F CSTVLQYQLLSVINPQIFLAFCARLTHFLHFLCVKMGFFGVLVLLNHQFLVIHLAINALHGRKARKKWKALRSVAAMHL *F KTLRLARRIFDMFDIANATVFINMFMTAINILYHAVQYSNSSIKSNGWGILFGNGLIVFNFWGTMALMEMLDSVVTSCN *F NTGQQLRQLSDLPKVGPKMQRELDVFTMQLRQNRLVYKICGIVELDKPACLSYIGSILSNVIILMQFDLRRQRQPINDR *F QYLIHLMKNKTKV *F >Gr58c *F MNQYFLLHTYFQVSRLIGLCNLHYDSSNHRFILNHVPTVVYCVILNVVYLLVLPFALFVLTGNIYHCPDAGMFGVVYNV *F VALTKLLTMLFLMSSVWIQRRRLYKLGNDLMKMLHKFRFNLGNDCRNRCLCKGLLTSSRFVLLTQQLLTRDSVVNCESN *F SSLRQAMVPYQSAAIVYALIMILLMSYVDMTVYMVEVAGNWLLVNMTQGVREMVQDLEVLPERNGIPREMGLMQILAAW *F RKLWRRCRRLDALLKQFVDIFQWQVLFNLLTTYIFSIAVLFRLWIYLEFDKNFHLWKGILYAIIFLTHHVEIVMQFSIF *F EINRCKWLGLLEDVGNLWDINYSGRQCIKSSGTILSRKLEFSLLYMNRKLQLNPKRVRRLHIVGLFDLSNLTVHNMTRS *F IITNVLVLCQIAYKKYG *F >Gr59a *F MKRIGQAYNVYAVFIGMTSYETMGGKFRQSRITRIYCLLINAIFLTLLPSAFWKSAKLLSTADWMPSYMRVTPYIMCTI *F NYAAIAYTLISRCYRDAMLMDLQRIVLEVNREMLRTGKKMNSLLRRMFFLKTFTLTYSCLSYILAVFIYQWKAQNWSNL *F CNGLLVNISLTILFVNTFFYFTSLWHIARGYDFVNQQLNEIVACQSMDLERKSKELRGLWALHRNLSYTARRINKHYGP *F QMLAMRFDYFIFSIINACIGTIYSTTDQEPSLEKIFGSLIYWVRSFDFFLNDYICDLVSEYQMQPKFFAPESSMSNELS *F SYLIYESSTRLDLLVCGLYRVNKRKWLQMVGSIVVHSSMLFQFHLVMRGGL *F >Gr59b *F MVYWMIKLYFRYSLAIGITSQQFSNRKFFSTLFSRTYALIANIVTLIMLPIVMWQVQLVFQQKKTFPKLILITNNVREA *F VSFLVILYTVLSRGFRDTAFKEMQPLLLTLFREEKRCGFKGIGGVRRSLRILLFVKFFTLSWLCVTDVLFLLYSTDALI *F WVNVLRFFFKCNTNNILEMVPMGYFLALWHIARGFDCVNRRLDQIVKSKSTRKHRELQHLWLLHACLTKTALNINKIYA *F PQMLASRFDNFVNGVIQAYWGAVFTFDLSTPFFWVVYGSVQYHVRCLDYYLIDNMCDVAVEYHDSAKHSWSEVRWTKEI *F SSYVIYANSTKLQLWSCGLFQANRSMWFAMISSVLYYILVLLQFHLVMRK *F >Gr59c *F MVDLVKTILLIAYWYGLAVGVSNFEVDWLTGEAIATRRTTIYAAVHNASLITLLILFNLGNNSLKSEFISARYLHEYFF *F MLMTAVRISAVLLSLITRWYQRSRFIRIWNQILALVRDRPQVVRGRWYRRSIILKFVFCVLSDSLHTISDVSAQRKRIT *F ADLIVKLSLLATLTTIFNMIVCQYYLAMVQVIGLYKILLQDLRCLVRQAECICSIRNRRGGVYSIQCCSLADQLDLIAE *F RHYFLKDRLDEMSDLFQIQSLSMSLVYFFSTMGSIYFSVCSILYSSTGFGSTYWGLLLIVLSTASFYMDNWLSVNIGFH *F IRDQQDELFRVLADRTLFYRELDNRLEAAFENFQLQLASNRHEFYVMGLFKMERGRLIAMLSSVITHTMVLVQWEIQND *F ES *F >Gr59d *F MADLLKLCLRIAYAYGRLTGVINFKIDLKTGQALVTRGATLISVSTHLLIFALLLYQTMRKSVVNVMWKYANSLHEYVF *F LVIAGFRVVCVFLELVSRWSQRRTFVRLFNSFRRLYQRNPDIIQYCRRSIVSKFFCVTMTETLHIIVTLAMMRNRLSIA *F LALRIWAVLSLTAIINVIITQYYVATACVRGRYALLNKDLQAIVTESQSLVPNGGGVFVTKCCYLADRLERIAKSQSDL *F QELVENLSTAYEGEVVCLVITYYLNMLGTSYLLFSISKYGNFGNNLLVIITLCGIVYFVFYVVDCWINAFNVFYLLDAH *F DKMVKLLNKRTLFQPGLDHRLEMVFENFALNLVRNPLKLHMYGLFEFGRGTSFAVFNSLLTHSLLLIQYDVQNF *F >Gr59e *F MDSSYWENLLLTINRFLGVYPSGRVGVLRWLHTLWSLFLLMYIWTGSIVKCLEFTVEIPTIEKLLYLMEFPGNMATIAI *F LVYYAVLNRPLAHGAELQIERIITGLKGKAKRLVYKRHGQRTLHLMATTLVFHGLCVLVDVVNYDFEFWTTWSSNSVYN *F LPGLMMSLGVLQYAQPVHFLWLVMDQMRMCLKELKLLQRPPQGSTKLDACYESAFAVLVDAGGGSALMIEEMRYTCNLI *F EQVHSQFLLRFGLYLVLNLLNSLVSICVELYLIFNFFETPLWEESVLLVYRLLWLAMHGGRIWFILSVNEQILEQKCNL *F CQLLNELEVCSSRLQRTINRFLLQLQRSIDQPLEACGIVTLDTRSLGGFIGVLMAIVIFLIQIGLGNKSLMGVALNRSN *F WVYV *F >Gr59f *F MRSSATKGAKLKNSPRERLSSFNPQYAERYKELYRTLFWLLLISVLANTAPITILPGCPNRFYRLVHLSWMILWYGLFV *F LGSYWEFVLVTTQRVSLDRYLNAIESAIYVVHIFSIMLLTWQCRNWAPKLMTNIVTSDLNRAYTIDCNRTKRFIRLQLF *F LVGIFACLAIFFNIWTHKFVVYRSILSINSYVMPNIISSISFAQYYLLLQGIAWRQRRLTEGLERELTHLHSPRISEVQ *F KIRMHHANLIDFTKAVNRTFQYSILLLFVGCFLNFNLVLFLVYQGIENPSMADFTKWVCMLLWLAMHVGKVCSILHFNQ *F SIQNEHSTCLTLLSRVSYARKDIQDTITHFIIQMRTNVRQHVVCGVINLDLKFLTTLLVASADFFIFLLQYDVTYEALS *F KSVQGNVTRYK *F >Gr61a *F MSRTSDDIRKHLKVRRQKQRAILAMRWRCAQGGLEFEQLDTFYGAIRPYLCVAQFFGIMPLSNIRSRDPQDVKFKVRSI *F GLAVTGLFLLLGGMKTLVGANILFTEGLNAKNIVGLVFLIVGMVNWLNFVGFARSWSHIMLPWSSVDILMLFPPYKRGK *F RSLRSKVNVLALSVVVLAVGDHMLYYASGYCSYSMHILQCHTNHSRITFGLYLEKEFSDIMFIMPFNIFSMCYGFWLNG *F AFTFLWNFMDIFIVMTSIGLAQRFQQFAARVGALEGRHVPEALWYDIRRDHIRLCELASLVEASMSNIVFVSCANNVYV *F ICNQALAIFTKLRHPINYVYFWYSLIFLLARTSLVFMTASKIHDASLLPLRSLYLVPSDGWTQEVQRFADQLTSEFVGL *F SGYRLFCLTRKSLFGMLATLVTYELMLLQIDAKSHKGLRCA *F >Gr63a *F MRPSGEKVVKGHGQGNSGHSLSGMANYYRRKKGDAVFLNAKPLNSANAQAYLYGVRKYSIGLAERLDADYEAPPLDRKK *F SSDSTASNNPEFKPSVFYRNIDPINWFLRIIGVLPIVRHGPARAKFEMNSASFIYSVVFFVLLACYVGYVANNRIHIVR *F SLSGPFEEAVIAYLFLVNILPIMIIPILWYEARKIAKLFNDWDDFEVLYYQISGHSLPLKLRQKAVYIAIVLPILSVLS *F VVITHVTMSDLNINQVVPYCILDNLTAMLGAWWFLICEAMSITAHLLAERFQKALKHIGPAAMVADYRVLWLRLSKLTR *F DTGNALCYTFVFMSLYLFFIITLSIYGLMSQLSEGFGIKDIGLTITALWNIGLLFYICDEAHYASVNVRTNFQKKLLMV *F ELNWMNSDAQTEINMFLRATEMNPSTINCGGFFDVNRTLFKGLLTTMVTYLVVLLQFQISIPTDKGDSEGANNITVVDF *F VMDSLDNDMSLMGASTLSTTTVGTTLPPPIMKLKGRKG *F >Gr64a *F MKGPNLNFRKTPSKDNGVKQVESLARPETPPPKFVEDSNLEFNVLASEKLPNYTNLDLFHRAVFPFMFLAQCVAIMPLV *F GIRESNPRRVRFAYKSIPMFVTLIFMIATSILFLSMFTHLLKIGITAKNFVGLVFFGCVLSAYVVFIRLAKKWPAVVRI *F WTRTEIPFTKPPYEIPKRNLSRRVQLAALAIIGLSLGEHALYQVSAILSYTRRIQMCANITTVPSFNNYMQTNYDYVFQ *F LLPYSPIIAVLILLINGACTFVWNYMDLFIMMISKGLSYRFEQITTRIRKLEHEEVCESVFIQIREHYVKMCELLEFVD *F SAMSSLILLSCVNNLYFVCYQLLNVFNKLRWPINYIYFWYSLLYLIGRTAFVFLTAADINEESKRGLGVLRRVSSRSWC *F VEVERLIFQMTTQTVALSGKKFYFLTRRLLFGMAGTIVTYELVLLQFDEPNRRKGLQPLCA *F >Gr64b *F MPQGETFHRAVSNVLFISQIYGLLPVSNVRALDVADIRFRWCSPRILYSLLIGILNLSEFGAVINYVIKVTINFHTSST *F LSLYIVCLLEHLFFWRLAIQWPRIMRTWHGVEQLFLRVPYRFYGEYRIKRRIYIVFTIVMSSALVEHCLLLGNSFHLSN *F MERTQCKINVTYFESIYKWERPHLYMILPYHFWMLPILEWVNQTIAYPRSFTDCFIMCIGIGLAARFHQLYRRIAAVHR *F KVMPAVFWTEVREHYLALKRLVHLLDAAIAPLVLLAFGNNMSFICFQLFNSFKNIGVDFLVMLAFWYSLGFAVVRTLLT *F IFVASSINDYERKIVTALRDVPSRAWSIEVQRFSEQLGNDTTALSGSGFFYLTRSLVLAMGTTIITYELMISDVINQGS *F IRQKTQYCREY *F >Gr64c *F MQQSGQKGTRNTLQHAIGPVLVIAQFFGVLPVAGVWPSCRPERVRFRWISLSLLAALILFVFSIVDCALSSKVVFDHGL *F KIYTIGSLSFSVICIFCFGVFLLLSRRWPYIIRRTAECEQIFLEPEYDCSYGRGYSSRLRLWGVCMLVAALCEHSTYVG *F SALYNNHLAIVECKLDANFWQNYFQRERQQLFLIMHFTAWWIPFIEWTTLSMTFVWNFVDIFLILICRGMQMRFQQMHW *F RIRQHVRQQMPNEFWQRIRCDLLDLSDLLGIYDKELSGLIVLSCAHNMYFVCVQIYHSFQSKGNYADELYFWFCLSYVI *F IRVLNMMFAASSIPQEAKEISYTLYEIPTEFWCVELRRLNEIFLSDHFALSGKGYFLLTRRLIFAMAATLMVYELVLIN *F QMAGSEVQKSFCEGGVGSSKSIFS *F >Gr64d *F MLRSHLSVHGLQMERSVQENTLHYTIGHVLIIARIFGVLPLAGINPNGKPENVRFRWFSPYILFFVVAFTFVIADFMLS *F TKIVLNDGLQLYTMGSLSFSVICIFCFGSFIKLSRRWPHIIRETALCERIFLKPCYANQEGLNFTRFLRRWALILLVAA *F LCEHLTYVGSAAWSNYVQIRDCNLKVGFVENYFLRERQELFSVFEYRAWMVFFIEWNTMAMTFVWNFGDIFLFLMCRGL *F KIRFQQLHWRIRQNLGKPMAKEFWQEIRSDFLDLDSLLKLYDKELSGLILVCCAHNMYFICVQVYHSFQVKGAFMDELY *F FWFCLLYVISRLMNMMLAASSIPQEIKDISNTLYEVRSSPWCDELGRLSEMLRNETFALSGMGYFYVTRRLIFAMAGAL *F MGYELVLFRQMQGAVVQKSICSRGPGSSMSIFFS *F >Gr64e *F MSRIKFWRRSRVGSEATLGIIKYRVVEKDTKRFKLSLIKAWLLRIRQEDYKYSGSFQEAIKPVLIIAQIFALMPVRKVS *F SKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFYLSIFLISLSFFQLARKWPEIAQSWQL *F VEAKLPPLKLPKERRSLAQHINMITIVATTCSLVEHIMSMLSMGYYVNSCPRWPDRPIDSFLYLSFSSVFYFVDYTRFL *F GIVGKVVNVLSTFAWNFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEEVDDAISTITL *F LCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESKRPLVIFRLVPREYWCDELKRFSE *F EVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILLQFNGEEKVPGCFEN *F >Gr64f *F MKILPKLERKLRRLKKRVTRTSLFRKLDLVHERLDMVLDQTELSRSDKEAFLSDGSFHQAVGRVLLVAEFFAMMPVKGV *F TGKHPSDLSFSWRNIRTCFSLLFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGSVLLVLIVALNLARQWPQLMMYWH *F TVEKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQNYFLRTNDEIFFVTSYSTTL *F ALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNFKGMNMAPSYWSERRIQYRNICILCDKMDDAISLITM *F VSFSNNLYFICVQLLRSLNTMPSVAHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTLRYLRCVPKESWCPEVKRFTE *F EVISDEVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS *F >Gr65a *F MRVHQRQSAVIIQMGHPPFMSLKGGKSGFGSIVWPSAMREVNLLNRFTRQFLFLIVLVTQICGVATFVYNSKAQCFRQS *F GFLRFYSSLVLIFLALFLIVTTSKMFHNLQAVWPYVVGSVIILVVRIHGLLESAEIVELLNQMLRIMRQVNLMARHPNL *F FRLKHLLLLLLALQNLLRSLNTIVGISNHSAEAYDSFLNSVILLIILAVLLSFLLQITINICLFVVLIATYSELHHCTR *F RISNDMDKLRLHSVHESGQFMVLVKQLQGITEKLIRLRQNVFHITVRIIRHFRFHWLCAIIYGLLPFFSLTAKDQNGFN *F FLIISALNIIFQWTIFAILSRESRITRSLCTFHLTNYHKETARTIDELLHQEIWERITVTIYGNTLDTKLLFKLLSISA *F FCAFVNRLEYLHI *F >Gr66a *F MDNMAQAEDAVQPLLQQFQQLFFISKIAGILPQDLEKFRSRNLLEKSRNGMIYMLSTLILYVVLYNILIYSFGEEDRSL *F KASQSTLTFVIGLFLTYIGLIMMVSDQLTALRNQGRIGELYERIRLVDERLYKEGCVMDNSTIGRRIRIMLIMTVIFEL *F SILVSTYVKLVDYSQWMSLLWIVSAIPTFINTLDKIWFAVSLYALKERFEAINATLEELVDTHEKHKLWLRGNQEVPPP *F LDSSQPPQYDSNLEYLYKELGAIDAASRKPPPPPLATNMVHESELGNAAKVEEKLNNLCQVHDEICEIGKALNELWSYP *F ILSLMAYGFLIFTAQLYFLYCATQYQSIPSLFRSAKNPFITVIVLSYTSGKCVYLIYLSWKTSQASKRTGISLHKCGVV *F ADDNLLYEIVNHLSLKLLNHSVDFSACGFFTLDMETLYGVSGGITSYLIILIQFNLAAQQAKEAIQTFNSLNDTAGLVG *F AATDMDNISSTLRDFVTTTMTPAV *F >Gr68a *F MKIYQDIYPISKPSQIFAILPFYSGDVDDGFRFGGLGRWYGRLVALIILIGSLTLGEDVLFASKEYRLVASAQGDTEEI *F NRTIETLLCIISYTMVVLSSVQNASRHFRTLHDIAKIDEYLLANGFRETYSCRNLTILVTSAAGGVLAVAFYYIHYRSG *F IGAKRQIILLLIYFLQLLYSTLLALYLRTLMMNLAQRIGFLNQKLDTFNLQDCGHMENWRELSNLIEVLCKFRYITENI *F NCVAGVSLLFYFGFSFYTVTNQSYLAFATLTAGSLSSKTEVADTIGLSCIWVLAETITMIVICSACDGLASEVNGTAQI *F LARIYGKSKQFQNLIDKFLTKSIKQDLQFTAYGFFSIDNSTLFKIFSAVTTYLVILIQFKQLEDSKVEDISQA *F >Gr77a *F MPLPLGDPLALAVSPQLGYIRITAMPRWLQLPGMSALGILYSLTRVFGLMATANWSPRGIKRVRQSLYLRIHGCVMLIF *F VGCFSPFAFWCIFQRMAFLRQNRILLMIGFNRYVLLLVCAFMTLWIHCFKQAEIIGCLNRLLKCRRRLRRLMHTRKLKD *F SMDCLATKGHLLEVVVLLSSYLLSMAQPIQILKDDPEVRRNFMYACSLVFVSVCQAILQLSLGMYTMAILFLGHLVRHS *F NLLLAKILADAEHIFESSQKAGFWPNRQELYKGQQKWLALELWRLLHVHHQLLKLHRSICSLCAVQAVCFLGFVPLECT *F IHLFFTYFMKYSKFILRKYGRSFPLNYFAIAFLVGLFTNLLLVILPTYYSERRFNCTREIIKGGGLAFPSRITVKQLRH *F TMHFYGLYLKNVEHVFAVSACGLFKLNNAILFCIVGAILEYLMILIQFDKVLNK *F >Gr85a *F MYSLIEAQLLGGKLVNRVMASLRRIIQRSLGYFCALNGILDFNTDIGTGNLRRYRVLFMYRLLHNFAVISLTLKFLFDF *F TDHFKYIESSTLITVNFFTYFTLVFFALLSSMGSCYQWQNRILAVLKELKHQRDLSRHMGYRVPRSKQNSIDYLLFALT *F VLLILRLSIHLATFTLSARMGFNHPCNCFLPECMIFSMNYLLFAILAEITRCWWSLQSGLKMVLLNRQLSTVAFNLWEI *F ERLHTRFQCLIDLTSEVCSIFRYVTLAYMARNLWSGIVAGYLLVRFVIGNGLQDVELVYLVFSFITCIQPLMLSLLVNS *F MTSTTGSLVEVTRDILKISHKKSVNLERSIEWLSLQLTWQHTHVTIFGVFRINRSLAFRSASLILVHVLYMVQSDYISI *F TN *F >Gr92a *F MFEFLHQMSAPKLSTSILRYIFRYAQFIGVIFFCLHTRKDDKTVFIRNWLKWLNVTHRIITFTRFFWVYIASISIKTNR *F VLQVLHGMRLVLSIPNVAVILCYHIFRGPEIIDLINQFLRLFRQVSDLFKTKTPGFGGRRELILILLNLISFAHEQTYL *F WFTIRKGFSWRFLIDWWCDFYLVSATNIFIHINSIGYLSLGVLYSELNKYVYTNLRIQLQKLNTSGSKQKIRRVQNRLE *F KCISLYREIYHTSIMFHKLFVPLLFLALIYKVLLIALIGFNVAVEFYLNSFIFWILLGKHVLDLFLVTVSVEGAVNQFL *F NIGMQFGNVGDLSKFQTTLDTLFLHLRLGHFRVSILGLFDVTQMQYLQFLSALLSGLAFIAQYRMQVGNG *F >Gr93a *F MFSSSSAMTGKRAESWSRLLLLWLYRCARGLLVLSSSLDRDKLQLKATKQGSRNRFLHILWRCIVVMIYAGLWPMLTSA *F VIGKRLESYADVLALAQSMSVSILAVISFVIQARGENQFREVLNRYLALYQRICLTTRLRHLFPTKFVVFFLLKLFFTL *F CGCFHEIIPLFENSHFDDISQMVGTGFGIYMWLGTLCVLDACFLGFLVSGILYEHMANNIIAMLKRMEPIESQDERYRM *F TKYRRMQLLCDFADELDECAAIYSELYHVTNSFRRILQWQILFYIYLNFINICLMLYQYILHFLNDDEVVFVSIVMAFV *F KLANLVLLMMCADYTVRQSEVPKKLPLDIVCSDMDERWDKSVETFLGQLQTQRLEIKVLGFFHLNNEFILLILSAIISY *F LFILIQFGITGGFEASEDIKNRFD *F >Gr93b *F MVYGFTMSGLLVMPRILRCLNVSRISAILLRSCFLYGTFFGVITFRIERKDSQLVAINRRGYLWICLVIRLLASCFYGY *F SYDAWSGQYEDMYLRAFFGFRLIGCLICSVIILVMQFWFGEELINLVNRFLQLFRRMQSLTNSPKNRFGDRAEFLLMFS *F KVFSLLFVFMAFRLMLSPWFLLTLVCDLYTSVGTGMITHLCFVGYLSIGVLYRDLNNYVDCQLRAQLRSLNGENNSFRN *F NPQPTRQAISNLDKCLYLYDEIHQVSRSFQQLFDLPLFLSLAQSLLAMSMVSYHAILRRQYSFNLWGLVIKLLIDVVLL *F TMSVHSAVNGSRLIRRLSFENFYVTDSQSYHQKLELFLGRLQHQELRVFPLGLFEVSNELTLFFLSAMVTYLVFLVQYG *F MQSQQI *F >Gr93c *F MIERLKKVSLPALSAFILFCSCHYGRILGVICFDIGQRTSDDSLVVRNRHQFKWFCLSCRLISVTAVCCFCAPYVADIE *F DPYERLLQCFRLSASLICGICIIVVQVCYEKELLRMIISFLRLFRRVRRLSSLKRIGFGGKREFFLLLFKFICLVYELY *F SEICQLWHLPDSLSLFATLCEIFLEIGSLMIIHIGFVGYLSVAALYSEVNSFARIELRRQLRSLERPVGGPVGRKQLRI *F VEYRVDECISVYDEIERVGRTFHRLLELPVLIILLGKIFATTILSYEVIIRPELYARKIGMWGLVVKSFADVILLTLAV *F HEAVSSSRMMRRLSLENFPITDHKAWHMKWEMFLSRLNFFEFRVRPLGLFEVSNEVILLFLSSMITYFTYVVQYGIQTN *F RL *F >Gr93d *F MKATKYSVGILRFMSFYARFLSLVCFRLRKQKDNNVWLEEIWSNRSRWKWISVTLRIVPLCIYAFTYAEWISNRMLITE *F KFLHSCSLVVSIPCYLSIIHLKICHGPEVTKLVNQYLHIFRLGTLDIRRRSQFGGGRELFLLILSVCCQIHEYVFILVI *F ASRLCGFQHIIWWVSYTYVFIICNSIMCFGFIWHLSLGVLYAELNDNLRFESGFQTAFLRKQQRIRVQKSMALFKEISS *F VVTSLQDIFNVHLFLSALLTLLQVLVVWYKMIIDLGFSDFRIWSFSLKNLIQTLLPVLAIQEAANQFKQTRERALDIFL *F VGKSKHWMKSVEIFVTHLNLSEFRVNLLGLFNVSNELFLIIVSAMFCYLVFVTQCVIVYRRRYVI *F >Gr94a *F MDFTSDYAHRRMVKFLTIILIGFMTVFGLLANRYRAGRRERFRFSKANLAFASLWAIAFSLVYGRQIYKEYQEGQINLK *F DATTLYSYMNITVAVINYVSQMIISDHVAKVLSKVPFFDTLKEFRLDSRSLYISIVLALVKTVAFPLTIEVAFILQQRR *F QHPEMSLIWTLYRLFPLIISNFLNNCYFGAMVVVKEILYALNRRLEAQLQEVNLLQRKDQLKLYTKYYRMQRFCALADE *F LDQLAYRYRLIYVHSGKYLTPMSLSMILSLICHLLGITVGFYSLYYAIADTLIMGKPYDGLGSLINLVFLSISLAEITL *F LTHLCNHLLVATRRSAVILQEMNLQHADSRYRQAVHGFTLLVTVTKYQIKPLGLYELDMRLISNVFSAVASFLLILVQA *F DLSQRFKMQ *F >Gr97a *F MRFLRRQTRRLRSIWQRSLPVRFRRGKLHTQLVTICLYATVFLNILYGVYLGRFSFRRKKFVFSKGLTIYSLFVATFFA *F LFYIWNIYNEISTGQINLRDTIGIYCYMNVCVCLFNYVTQWEKTLQIIRFQNSVPLFKVLDSLDISAMIVWRAFIYGLL *F KIVFCPLITYITLILYHRRSISESQWTSVTTTKTMLPLIVSNQINNCFFGGLVLANLIFAAVNRKLHGIVKEANMLQSP *F VQMNLHKPYYRMRRFCELADLLDELARKYGFTASRSKNYLRFTDWSMVLSMLMNLLGITMGCYNQYLAIADHYINEEPF *F DLFLAIVLVVFLAVPFLELVMVARISNQTLVETRRTGELLQRFDLQHADARFKQVVNAFWLQVVTINYKLMPLGLLELN *F TSLVNKVFSSAIGSLLILIQSDLTLRFSLK *F >Gr98a *F MEQMSGELHAASLLYMRRLMKCLGMLPFGQNLFSKGFCYVLLFVSLGFSSYWRFSFDYEFDYDFLNDRFSSTIDLSNFV *F ALVLGHAIIVLELLWGNCSKDVDRQLQAIHSQIKLQLGTSNSTDRVRRYCNWIYGSLIIRWLIFIVVTIYSNRALTINA *F TYSELVFLARFSEFTLYCAVILFIYQELIVGGSNVLDELYRTRYEMWSIRRLSLQKLAKLQAIHNSLWQAIRCLECYFQ *F LSLITLLMKFFIDTSALPYWLYLSRVEHTRVAVQHYVATVECIKLLEIVVPCYLCTRCDAMQRKFLSMFYTVTTDRRSS *F QLNAALRSLNLQLSQEKYKFSAGGMVDINTEMLGKFFFGMISYIVICIQFSINFRAKKMSNEQMSQNITSTSAPI *F >Gr98b *F MVAQKSRLLARAFPYLDIFSVFALTPPPQSFGHTPHRRLRWYLMTGYVFYATAILATVFIVSYFNIIAIDEEVLEYNVS *F DFTRVMGNIQKSLYSIMAIANHLNMLINYRRLGGIYKDIADLEMDMDEASQCFGGQRQRFSFRFRMALCVGVWMILMVG *F SMPRLTMTAMGPFVSTLLKILTEFVMIMQQLKSLEYCVFVLIIYELVLRLRRTLSQLQEEFQDCEQQDMLQALCVALKR *F NQLLLGRIWRLEGDVGSYFTPTMLLLFLYNGLTILHMVNWAYINKFLYDSCCQYERFLVCSTLLVNLLLPCLLSQRCIN *F AYNCFPRILHKIRCTSADPNFAMLTRGLREYSLQMEHLKLRFTCGGLFDINLKYFGGLLVTIFGYIIILIQFKVQAIAA *F NRYKKVVN *F >Gr98c *F MEMEAKRSRLLTTARPYLQVLSLFGLTPPAEFFTRTLRKRRRFCWMAGYSLYLIAILLMVFYEFHANIVSLHLEIYKFH *F VEDFSKVMGRTQKFLIVAIATCNQLNILLNYGRLGLIYDEIANLDLGIDKSSKNFCGKSHWWSFRLRLTLSIGLWMVII *F IGVIPRLTLGRAGPFFHWVNQVLTQIILIMLQLKGPEYCLFVLLVYELILRTRHVLEQLKDDLEDFDCGARIQELCVTL *F KQNQLLIGRIWRLVDEIGAYFRWSMTLLFLYNGLTILHVVNWAIIRSIDPNDCCQLNRLGSITFLSFNLLLTCFFSECC *F VKTYNSISYILHQIGCLPTAEEFQMLKMGLKEYILQMQHLKLLFTCGGLFDINIKLFGGMLVTLCGYVIIIVQFKIQDF *F ALIGYRQNTSDTS *F >Gr98d *F MEANRSRLLAAARPYIQIYSIFGLTPPIQFFTRTLHKRRRGIVILGYACYLISISLMVIYECYANIVALQKDIHKFHAE *F DSSKVMGNTQKVLVVAMFVWNQLNILLNFRRLARIYDDIADLEIDLNNASSGFVGQRHWWRFRFRLALSVGLWIVLLVG *F LTPRFTLVALGPYLHWTNKVLTEIILIMLQLKCTEYCVFVLLIYELILRGRHILQQISVELEGNQSRDSVQELCVALKR *F NQLLAGRIWGLVNEVSLYFTLSLTLLFLYNELTILQIVNWALIKSVNPNECCQYRRVGTCLLLSINIFLSCLYSEFCIQ *F TYNSISRVLHQMYCLSAAEDYLILKMGLREYSLQMEHLKLIFTCGGLFDINLKFFGGMVVTLFGYIIILVQFKIQFFAQ *F SNFMQNINSTELKAYTA *F \------------------------------------------------------------------------------ *F -- # *U FBrf0141794 *a Lohe *b A. *t 2001.11.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Nov 2001 17:14:33 \+1100 *F To: Gillian Millburn (Genetics) *F From: Allan Lohe *F Subject: Re: FlyBase query *F Hi Gillian, *F I made the constructs, and the transformants, (in 1983), both of which are *F unpublished. The w+ sequence is an EcoR1 fragment from a lambda phage (a *F gift of K. Burtis) inserted into a Carnegie vector. I can look up more *F details if you wish, but from memory a similar w+ fragment was first shown *F to rescue w- in a 1984 Cell paper by Tulle Hazelrigg. I'd also have to *F look up which of 'P{wA}' or 'P{C1}' my fragment would correspond to. The Y *F insertion was isolated as a secondary P transposase-induced jump. *F Please get back to me if there is any more information you might require. *F Regards, Allan *F At 17:35 31/10/01 \+0000, you wrote: *F >Dear Dr. Lohe, *F > *F >I am curating a paper for FlyBase: *F > *F >Haller and Woodruff, 2000, Genome 43(2): 285--292 *F > *F >in which they use a Pw+ element on the Y chromosome that you *F >isolated. The insertion is called 'Pw+YAL' in the paper. Do you know *F >which specific w+ transposon this is a Y-linked insert of \- in the *F >methods they say that the P-element carries a 11.7kb w+ sequence \- this *F >makes me think that the Pw+ element in this particular insert is *F >either 'P{wA}' or 'P{C1}'. *F > *F >These constructs are described in: *F > *F >Levis et al., 1985, EMBO J. 4(15A): 3489--3499 *F > *F >and both contain a 11.7kb w+ sequence. *F > *F >Do you know which transposon your Pw+YAL insertion contains ? *F > *F >I look forward to hearing from you, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F > # *U FBrf0141795 *a Schupbach *b T. *t 2001.10.19 *T personal communication to FlyBase *u *F From: 'Schupbach, Gertrud' *F To: ''Leyla Bayraktaroglu'' *F Subject: RE: grkWG41 (grk6) *F Date: Fri, 19 Oct 2001 17:54:37 \-0400 *F MIME-Version: 1.0 *F Dear Leyla, *F You are very correct, indeed, we made a mistake here, typing glu instead of *F gln. Thank you for alerting us. You are really doing a great job here! *F With best wishes *F Trudi *F > \---------- *F > From: Leyla Bayraktaroglu *F > Reply To: Leyla Bayraktaroglu *F > Sent: Friday, October 19, 2001 12:05 PM *F > To: gschupbach@molbio.princeton.edu *F > Cc: leyla@morgan.harvard.edu *F > Subject: grkWG41 (grk6) *F > *F > Dear Trudi, *F > *F > I have completed mapping the grk mutations to the reference *F > sequence, and I noticed that the amino acid change given *F > for 'WG41' in figure 1 of the Thio et al. paper appears to have *F > a typo. In the figure, the change is shown as: *F > *F > WG41 *F > bp 2217 *F > G->A *F > arg to glu *F > *F > And FlyBase curators curated that amino acid change as *F > arg to glu (R?E). *F > *F > But as far as I can tell from the map I made, the amino acid *F > change should be: *F > arg to gln (R221Q). CGG \-> CAG *F > *F > I thought I'd bring this to your attention. Please let me *F > know if I've made a mistake! *F > *F > Thanks. *F > *F > Leyla *F > *F > *F \------------- End Forwarded Message \------------- # *U FBrf0141806 *a Deal *b J. *c K. *d Cook *t 2001.12.6 *T personal communication to FlyBase *u *F Date: Thu, 06 Dec 2001 13:27:21 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC8 *F Isolation and characterization of Df(3L)BSC8 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Df(2L)BSC8 was isolated as a P transposase-induced male recombination event *F involving P{lacW}l(3)j11B2j11B2 and P{PZ}Eip75B07041. The deletion was *F isolated as a rho+-e+ recombinant chromosome from the cross rhove-1 *F pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{PZ}Eip75B07041/P{lacW}l(3)j11B2j11B2 e1 males. Polytene chromosome *F squashes showed the breakpoints 74D3-75A1;75B2-5. Df(2L)BSC8 failed to *F complement Eip74EFDL-1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141807 *a Goto *b A. *t 2001.12.6 *T personal communication to FlyBase *u *F Date: Fri, 07 Dec 2001 08:46:48 \-0500 *F To: Gillian Millburn (Genetics) *F From: Kevin Cook *F Subject: P{Hml-GAL4.G} construct and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Akira Goto, Nagoya University (11/01). *F The identification and characterization of Hml was described in Goto et al. *F 2001, Biochem J. 359: 99-108 FBrf0138238. *F pP{Hml-GAL4.G} was constructed by combining a GAL4-spanning BamHI-NotI *F fragment excised from pGaTB and the 3.0 kb upstream promoter region of Hml *F and cloning them into pP{CaSpeR-4}. The promoter region of Hml flanked by *F BamHI sites was prepared by amplification of genomic DNA using primers *F 5'-CGCGGATCCAAGAACTTTACATAAATTCCGGTATC-3' and *F 5'-CGCGGATCCTTTGTTAGGCTAATCGGAAATTGTTGG-3' based on the sequence nt *F 20501-23450 in GenBank AC015143. *F Insertions of P{Hml-GAL4.G} include the following: *F P{Hml-GAL4.G}5-6 homozygous viable and fertile chromosome 3 insertion *F P{Hml-GAL4.G}6-4 homozygous viable and fertile chromosome 2 insertion *F P{Hml-GAL4.G}8-5 homozygous viable and fertile chromosome 3 insertion *F P{Hml-GAL4.G}13-6 homozygous viable and fertile chromosome 3 insertion *F Endogenous Hml is expressed at low levels in larval hemocytes. A second *F chromosome with P{Hml-GAL4.G}6-4, P{UAS-GFP::lacZ.nls} and P{UAS-GFP.S65T} *F insertions gave weak but detectable GFP expression when heterozygous, and *F strong GFP expression when homozygous. GFP expression was not detectable *F when a P{Hml-GAL4.G} insertion was crossed to single UAS-GFP construct *F insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141808 *a Tomkiel *b J. *t 2001.12.6 *T personal communication to FlyBase *u *F Date: Thu, 06 Dec 2001 16:19:14 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Df(2R)P803-delta15 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by John Tomkiel, Wayne State U. (11/01). *F Df(2R)P803-delta15 was recovered as transposase-mediated male recombination *F event in P{lacW}l(2)k08805k08805/cn1 bw1; delta2-3 fathers. The *F deletion chromosome carries cn1, and the deletion extends from the 3' end *F of P{lacW}. The P element-associated w+ eye color marker is still *F present as judged by phenotype, and the 5' and 3' ends of the P element are *F present and non-rearranged as determined by DNA sequencing. Sequencing of *F an inverse PCR product generated after MspI digestion of genomic DNA *F indicates that the P element is now adjacent to the sequence *F GGTTTAGCAATTATTACTTTATTT, which is within the coding sequence of the Ark *F gene at 53E. *F The chromosome fails to complement the recessive lethality of the *F P{lacW}l(2)k08805k08805 parental chromosome, P{lacW}l(2)k15914k15914 *F and teflon. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141809 *a Matthews *b B. *t 2001.12.6 *T personal communication to FlyBase *u *F Date: Thu, 6 Dec 2001 11:03:26 \-0500 (EST) *F From: Beverley Matthews *F Subject: gene merge *F To: flybase-cambridge@morgan.harvard.edu *F Hi Gillian, *F I thought I should send you this merge sooner rather than have it come *F down the apollo pipeline. *F Trfp has already been merged with CG18267 which is fine (I guess). It *F turns out there is a duplicate annotation (same transcript, same *F location) in GadFly under the gene symbol CG18780. Please merge them *F all under Trfp. I don't know how the same gene got two different CG *F names in the first place. It's only has a single exon. Anyway, there *F you have it. *F Bev *F Here's the alignment of Trfp and CG18780 from GadFly *F Sequence 1 *F lcl|tmpseq_0 GO: located on: 2L 28E3-28E3; |cDNA sequence *F Length 835(1 .. 835) *F Sequence 2 *F lcl|tmpseq_1 GO: located on: 2L 28E3-28E3; |cDNA sequence *F Length 835(1 .. 835 *F Query: 1 cactggacgcttttagcttgcaagacttgtaaacaaaattaataaaactaaaaagctgac 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 cactggacgcttttagcttgcaagacttgtaaacaaaattaataaaactaaaaagctgac 60 *F Query: 61 attattgcataaactatgggagtaactatccttcaaccgtatcccttacccgaaggcaaa 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 attattgcataaactatgggagtaactatccttcaaccgtatcccttacccgaaggcaaa 120 *F Query: 121 tcgggtgcccacataatcgatcagctgagcaagcgtctgctcgccctgggcgccacacat 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 tcgggtgcccacataatcgatcagctgagcaagcgtctgctcgccctgggcgccacacat 180 *F Query: 181 gccggtcaatttctggtggactgcgaaacgttcatctcgacgccacagccgcacaatgga 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 gccggtcaatttctggtggactgcgaaacgttcatctcgacgccacagccgcacaatgga 240 *F Query: 241 gcacctggacgcgcagtccacgtcctgcacaactccgagtatcccgcctccacgttctcg 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 gcacctggacgcgcagtccacgtcctgcacaactccgagtatcccgcctccacgttctcg 300 *F Query: 301 atcatcgacaatggcaccggcaaacaagtggccattgtcgccgacaatatcttcgatctg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301 atcatcgacaatggcaccggcaaacaagtggccattgtcgccgacaatatcttcgatctg 360 *F Query: 361 ctcatgctcaagatgaccaacaccttcacctccaaaaagcagaccaaaatcgagtcccgt 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 361 ctcatgctcaagatgaccaacaccttcacctccaaaaagcagaccaaaatcgagtcccgt 420 *F Query: 421 ggcgctcgtttcgagtatggtgactttgttatcaaactcggttccgtcaccatgatggag 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 421 ggcgctcgtttcgagtatggtgactttgttatcaaactcggttccgtcaccatgatggag 480 *F Query: 481 cacttcaagggcatcctcatcgaaatcgagtacaagtcatgcgtgattctggcctactgc 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 481 cacttcaagggcatcctcatcgaaatcgagtacaagtcatgcgtgattctggcctactgc 540 *F Query: 541 tgggaaatgatacgcgaaatgctgcagggattcctcggcattgctgtgaataaggacttt 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 541 tgggaaatgatacgcgaaatgctgcagggattcctcggcattgctgtgaataaggacttt 600 *F Query: 601 ccctcctatttcgctccacagacaataatgacggcaatggggcaacagcagctgcatgcc 660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 601 ccctcctatttcgctccacagacaataatgacggcaatggggcaacagcagctgcatgcc 660 *F Query: 661 aagcacaacgacatctttgagccaatggacaccgtgaaacaatacctggagcagttcacc 720 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 661 aagcacaacgacatctttgagccaatggacaccgtgaaacaatacctggagcagttcacc 720 *F Query: 721 aactataggaagcatgtgactcttatgggcggtatgggcagtggaccgggtagccaacag 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 721 aactataggaagcatgtgactcttatgggcggtatgggcagtggaccgggtagccaacag 780 *F Query: 781 gttggtccgaacgtgcatatgtcgccggcggtggcgggaatacatcgatcctgat 835 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 781 gttggtccgaacgtgcatatgtcgccggcggtggcgggaatacatcgatcctgat 835 *F CPU time: 0.02 user secs. 0.06 sys. secs 0.08 total secs. *F Gapped *F Lambda K H *F 1.33 0.621 1.12 *F Gapped *F Lambda K H *F 1.33 0.621 1.12 *F Matrix: blastn matrix:1 \-2 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 1 *F Number of Sequences: 0 *F Number of extensions: 1 *F Number of successful extensions: 1 *F Number of sequences better than 10.0: 1 *F length of query: 835 *F length of database: 433,807,876 *F effective HSP length: 23 *F effective length of query: 812 *F effective length of database: 421,858,686 *F effective search space: 342549253032 *F effective search space used: 342549253032 *F T: 0 *F A: 30 *F X1: 6 (11.5 bits) *F X2: 26 (50.0 bits) *F S1: 12 (23.8 bits) *F S2: 18 (35.3 bits) # *U FBrf0141810 *a Robertson *b H. *c A. *d Chiba *e S. *f Hsu *t 2001.11.9 *T personal communication to FlyBase *u FlyBase error report for CadN on Fri Nov 9 12:50:26 2001. *F Date: Fri, 09 Nov 2001 12:50:26 \-0600 *F From: Hugh Robertson *F Subject: Re: annotation revisions *F To: Gillian Millburn *F cc: Chiba_Akira , Hsu_Shuning *F .. *F The gene (DN-Cadherin or CadN) as annotated in GadFly, FlyBase, and GenBank, *F uses a single cDNA from a Japanese group. These authors noted the possibility *F of one alternative splice, however we have carefully examined the gene and *F determined that there are three instances of alternative splicing. In each *F case these involve mutually exclusive use of one of two possible exons. *F Thus there is an alternative to the current exon 7, now called exon 7b, which *F is *F CGCGAGACATACAAGTTAGAGGCCATGGCCCAAGACAAGGGCTATCCGCCATTATCGCGTACTGTCGAAGTACAAATC *F GATGTGGTCGATCGTGCAAATAATCCGCCCGTCTGGGATCATACTGTCTATGGGCCCATTTATGTCAAGGAAAATATG *F CCCGTTGGCGGCAAAGTCGTCTCGATCAAAGCCAG. The alternative occurs in the intron *F before this exon, and therefore is called 7a, and the sequence is *F CGTGACCGTTATCGATTGAGGGTTAGTGCCTCCGATAAGGGCACTCCCGCCTCGGCAGCCGATGTCGATGTTGAGTTA *F GATGTCGTCGATCGGAATAATAAGCCACCCATTTGGGATAAGAGCATTTACGGGCCGATTCACATACGCGAGAATGTC *F ACTGTGGGTACGGTTGTAACATCGGTTAAGGCAAG *F Then there is an alternative exon for the current exon 13, which is now exon *F 13a, *F TATGAACTCCGTTTGGCCGCCTCCGACAATTTGAAGGAGAACTACACCACCGTGATAATCCACGTCAAGGATGTTAAC *F GATAATCCCCCCGTTTTTGAGCGACCCACTTATCGCACCCAGATTACCGAAGAGGACGATCGTAATTTGCCCAAACGC *F GTCTTGCAG *F that occurs in the intron after this, and it is therefore called 13b, *F TACGAACTGAAATTGGTTGCCTCGGACAGTTTGAATGAAAATCAAACAACAATCGTCATAAATGTGCGCGACGTCAAC *F GATCTTCCACCGCAATTCCCGCAGACATCCTATGAGCGAACTCTCGACGAGGGAATGACCAACACGCCCTTTACCATT *F ATGCAG *F Finally there is an alternative exon for the current exon 18, now called 18a, *F TTGCGGCGAAGGACGCATTATGTCGCCCGATTCCAAGGGCTGTATGGATCGCAACGAATGCCTCGATATGCCCTGCAT *F GAACGGTGCCACATGCATCAATCTCGAGCCCCGACTCCGATATCGTTGCATATGTCCAGATGGATTTTGGGGCGAGAA *F TTGCGAACTGGTGCAGGAGGGTCAGACCTTAAAACTCAGCATGGGCGCCCTGGCGGCGATTTTGGTCTGCCTTTTGAT *F CATACTGA *F that occurs in the intron after this, and it is therefore called 18b *F CTGTCCGGCGGGCTACAAAAGCTCGGGCAGCACCTGTGTCAACGACAACGAGTGCCTACTGTTCCCCTGCCGCAACGG *F GGGTCGCTGTCGCGATCATCATCCTCCAAAGAAGTACGAGTGCCATTGCCCCATGGGCTTCACCGGCATGCATTGCGA *F ACTGGAGTTATTGGCCTCCGGTGTATTGACGCCATCCCGTGATTTTATTGTCGCCCTGGCGCTCTGCCTGGGCACTCT *F GATAC *F My collaborators, Shuning Hsu and Akira Chiba, have RT/PCR evidence that all *F of these alternative exons are in fact employed in at least some transcripts, *F and we anticipate that all eight possible combinations occur, although we do *F not yet have cDNA evidence for all eight possible combinations. We therefore *F suggest that all eight potential splice forms be annotated for this gene, *F perhaps with the names CadNa, b, c, etc. In essence this is a very simple *F version of the kind of splicing found in the amazing Dscam gene. *F Hugh *F .. *F Hugh M. Robertson, Professor *F Department of Entomology, University of Illinois at Urbana-Champaign *F 320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801 *F Phone 217-333-0489; FAX 217-244-3499; email hughrobe@uiuc.edu *F WWW http://www.life.uiuc.edu/robertson/lab.html # *U FBrf0141811 *a Hutter *b P. *t 2001.11.19 *T personal communication to FlyBase *u *F Date: Wed, 7 Nov 2001 01:32:31 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: pierre.hutter@ichv.vsnet.ch *F Subject: FlyBase error report for LD22117 on Wed Nov 7 01:32:30 2001 *F Error report from Pierre HUTTER (pierre.hutter@ichv.vsnet.ch) *F Gene or accession: LD22117 *F Release: 2 *F cDNA or EST error *F Comments: In AE003451, nucleotides 34'106-34'699 contains an ORF which *F is 98% homologous with Ras small GTPases such as CG2532 gene. No gene *F is currently identified at this site, just 3' of LD22117 (CG1619). It *F is possible that this new Ras small GTPase gene should be merged with *F LD22117. this gene should be on the plus strand, between the annotated *F genes spri and BcDNA:LD22117. Evidence for the presence of a strong *F homology between this new gene and genes now known as CG2532, CG9807 *F and CG2885 (all encoding small GTPases) was reported in Hutter and *F Karch (Experientia 50:749-762, 1994). I should stress that this *F sequence is also present near the CG2111 gene, again where no Ras small *F GTPase gene is identified yet. *F Pierre Hutter *F Date: Mon, 19 Nov 2001 18:56:29 \+0600 *F To: gm119@gen.cam.ac.uk *F From: phutter *F Subject: cDNA Small Rab GTPase gene in AE003451 (nt 106042-106636) *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Dear Gillian, Dear Sima, *F Just one more thing. In my first e-mail about the new small GTPase gene *F between the annotated genes spri and BcDNA:LD22117, I had mentioned another *F nearby site which harbours an unidentified small Rab GTPase gene between *F CG9806 and CG2111 (on AE003451 scaffold as well). I cannot work out its *F genomic structure but when the AE003451 scaffold will be reannotated, nt *F 106042-106636 (referring to release 2.0) should definitely also be *F investigated for homologies to other Ras GTPases, EST sequences etc. *F .. *F Many thanks again for your assistance, Pierre *F Dr Pierre Hutter *F Unit of Genetics *F Institut Central des Hôpitaux Valaisans *F Av.Grand-Champsec 86 *F 1951 SION *F Switzerland *F PHONE:+41 27 603 47 57 *F FAX: \+41 27 603 48 40 *F E-mail: pierre.hutter@ichv.vsnet.ch # *U FBrf0141812 *a Robertson *b H. *t 2001.10.17 *T personal communication to FlyBase *u FlyBase error report for New gene on Wed Oct 17 13:59:01 2001. *F Date: Wed, 17 Oct 2001 13:59:01 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for New gene on Wed Oct 17 13:59:01 2001 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: New gene *F Release: 1 *F Missed gene *F Comments: Within the first intron of the current truncated annotation of *F DmNCad2, or CG7527, there is a short ORF in the same transcriptional *F orientation that encodes a 187aa protein with highly significant similarity to *F the two intron gene/protein CG8580, and then in turn convincing and *F significant similarity to a pair of mammalian proteins, and using PSI-BLASTP, *F eventually similarity to similar short proteins in C. elegans and perhaps *F plants and fungi. Seemingly a second member of a small family of short *F proteins of no known function. The amino acid sequence is *F MSHITRKRLLTLDEESQHINSTKRCGSNCIEKMLTKWSRQSIPGVNSISRKRSFPWEDNSPDIFPAKHSRKHIIKKKSK *F TIHEPEVPIDFLMGLPVLLDSDFSDSEECRPKSEIHVAKPETEDNRDLFTYQQLKLMCCEMMKQCEDRVVLEYEIALTQ *F KMAEQYDTFIKFNHDQLQRECEHRASYLS # *U FBrf0141813 *a Manning *b G. *t 2001.10.16 *T personal communication to FlyBase *u FlyBase error report for CG18355 on Tue Oct 16 10:25:39 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 16 Oct 2001 10:25:39 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: gerard-manning@sugen.com *F Subject: FlyBase error report for CG18355 on Tue Oct 16 10:25:39 2001 *F Error report from Gerard Manning (gerard-manning@sugen.com) *F Gene or accession: CG18355 *F Release: 2 *F Gene annotation error *F Genes CG18355 and Btk29A should be merged. *F Comments: Two splice variants of Btk29A have been published (Baba et al, MCB *F (1999), 19:4405-13). Unique 5' exons in the type II cDNA (Genbank gi|2723312) *F are identical to the sequence of CG18355 and both map to the same genomic locus. # *U FBrf0141814 *a Urban *b S. *t 2001.10.16 *T personal communication to FlyBase *u *F Date: Tue, 16 Oct 2001 22:17:41 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: sin@mrc-lmb.cam.ac.uk *F Subject: FlyBase error report for CG1004 on Tue Oct 16 22:17:41 2001 *F Error report from Sin Urban (sin@mrc-lmb.cam.ac.uk) *F Gene or accession: CG1004 *F Release: 2 *F Missed gene *F Comments: Although the precise role of Rhomboid in triggering EGF receptor *F signalling has remained elusive, we have now determined its mechanism. *F Rhomboid-1 is an intramembrane serine protease that cleaves the three *F TGFa-like EGF ligands in Drosophila, thus activating them as signals. The *F cleavage occurs within their transmembrane domains. Rhomboid-1 is localized *F in the Golgi apparatus, and this is the compartment where cleavage of the *F ligand occurs (not at the plasma membrane). The other fly Rhomboids (2,3, and *F 4) are also intramembrane serine proteases that catalyze the proteolytic *F release of these ligands. This work is published in the following articles: *F Urban, S, Lee J. R., and M. Freeman. Drosophila Rhomboid-1 defines a family *F of putative *F intramembrane serine proteases. (2001). Cell 107: in press Oct 19th issue. *F Lee J. R., Urban, S, Garvey C. F., and M. Freeman. Regulated intracellular *F transport and proteolysis control EGF ligand activation in Drosophila. (2001). *F Cell 107: in press Oct 19th issue. # *U FBrf0141815 *a Levis *b R. *t 2001.9.20 *T personal communication to FlyBase *u *F Date: Thu, 20 Sep 2001 13:32:15 \-0400 *F To: Gillian Millburn (Genetics) *F From: Robert Levis *F Subject: Should CG3364 and CG18742 be merged? *F Hi Gillian, *F Another pair of genes that share a common 5' end is CG3364 and *F CG18742. *F ...Bob # *U FBrf0141816 *a Whitfield *b E. *t 2001.11.20 *T personal communication to FlyBase *u FlyBase error report for CG3620 on Tue Nov 20 09:15:16 2001. *F Date: Tue, 20 Nov 2001 09:15:16 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG3620 on Tue Nov 20 09:15:16 2001 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG3620 *F Release: 2 *F DNA error at position 267044. Upstream nucleotides: gcaaggaaaaacggaatttt *F Substitution: base a should be g. *F Comments: The sequencing error shown means that the Cys required for the *F interchain *F disulfid bridge between norpA and inaD (as described in Kimple et al EMBO J. *F 20:4414-4422(2001)) is not present in the genome project translation as the *F Cys is a Tyr. *F Correct sequence DNA sequence is shown in J03138; AAA28724, Bloomquist et al, *F Cell 54:723-733(1988) and the correct amino acid sequencing in the Kimple et al *F paper. *F Also comparing J03138; AAA28724 to translation from genome project AE003430; *F AAF45942 shows the latter is missing a substantial amount of translation (I *F think due to intron-exon splice error as the exons are present in the mRNA *F feature). *F that's all! *F thanks # *U FBrf0141821 *a Adachi-Yamada *b T. *t 2001.12.10 *T personal communication to FlyBase *u *F Date: Mon, 10 Dec 2001 14:50:55 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-hep.CA}4 and P{UAS-bsk.DN}2 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Takashi Adachi-Yamada, Kobe University (12/01). *F P{UAS-hep.CA}4 is a homozygous viable and fertile, third chromosome insertion. *F P{UAS-bsk.DN}2 is a homozygous viable and fertile, X chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141822 *a Katz *b F. *t 2001.12.11 *T personal communication to FlyBase *u *F Date: Tue, 11 Dec 2001 11:53:59 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: fj and d alleles *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Flora Katz, Texas A&M University (9/01). *F fjN7 (FBal0127230) was derived from P{lacW}fj9-II (FBal0045640) by *F imprecise excision. It is a complex rearrangement that retains the lacZ *F marker. lacZ expression is stronger than in the parental enhancer *F trap. It gives a loss of cells between the crossveins of the wing similar *F to strong fj alleles. *F dGC2 is a weak hypomorph with respect to growth and segmentation, but *F gives a strong malformed leg phenotype. It was generated in Michael *F Hoffmann's lab by gamma mutagenesis. It has a deletion in a unique *F N-terminal pre-head domain that removes a leucine zipper. *F dGC13 was generated in the same irradiation screen and has an eleven base *F pair deletion in the myosin head domain that causes a frameshift and stop *F codon. It acts like a null allele. *F d210 (FBal0127240) was also generated in Michael Hoffmanns lab by EMS *F treatment. It has a point mutation causing a stop codon in the myosin head *F domain. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141823 *a Roote *b J. *t 2001.12.11 *T personal communication to FlyBase *u *F Date: Tue, 11 Dec 2001 10:22:20 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Balancer variant *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote, Cambridge University (12/01). *F A new balancer variant was constructed at Cambridge by recombination *F between FM7j (FBba0000227) and FM7i (FBba0000226). The recombinant *F chromosome resulted from a crossover between oc and B, and the resulting *F chromosome has the markers y93j sc8 w1 B1. The balancer short name *F of the variant is FM7j, B1. The balancer variant is viable and fertile *F in homozygotes and hemizygotes. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141825 *a Luo *b L. *t 2001.12.13 *T personal communication to FlyBase *u *F Date: Thu, 13 Dec 2001 09:57:00 \-0500 *F To: Rachel Drysdale (Genetics) , *F Gillian Millburn (Genetics) *F From: Kevin Cook *F Subject: Luo insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Liqun Luo, Stanford University (12/01). *F P{UAS-RhoGAPp190.N-dsRNA}8.2 is a homozygous viable and fertile second *F chromosome insertion. *F P{UAS-RhoGAPp190.GAP-dsRNA}5.2 is a homozygous viable and fertile third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141826 *a Dostert *b C. *c J.L. *d Imler *t 2001.12.18 *T personal communication to FlyBase *u FlyBase error report for CG13197 on Tue Dec 18 03:04:43 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 18 Dec 2001 03:04:43 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: JL.Imler@ibmc.u-strasbg.fr *F Subject: FlyBase error report for CG13197 on Tue Dec 18 03:04:43 2001 *F Error report from C. Dostert and JL. Imler (JL.Imler@ibmc.u-strasbg.fr) *F Gene or accession: CG13197 *F Release: 2 *F Gene annotation error *F Gene CG13197 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F GAGGATTATTTCTTTGCAAAACATTGTCAACAATTTTGAAGAACTTCGAAATTTTATTTTTAAATTTCGGTAAAAGGTTT *F GGCGAACAGACAAAATGGTCAAGGACATACCAGACAGATGGCTGAAGTACAAACCGATTGGAGATCGTGTTCCTGGCACT *F CGTTTCATAGCCTTTAAGGTGCCGCTGAATCAGCATGTCAACGCCAAAGTGAAGGAGAATCTTCGGCTGGCGCCCGAATC *F CCTTCTGCAGATCGTACCCGATATGGGTCTCATCATCGATTTGACCAACACGAATCGATACTACCACCCAAGTGCCATAA *F CCAACCACGATGTGCTCCACCAGAAGCTGATGATCCCCGGAAAACAGACACCCTCGCATAAGCTAGCCCAAAGATTCTGT *F GCTTTCGTGACTGACTTCCTTGAAAGGAACGCGGATAATGACAAACTAATTGGCGTCCATTGCACACATGGAGTAAATCG *F AACTGGATACCTGATCTGCTACTTCATGATCTCCGTAATGAACATGTCCCCTGAGGAGGCGATACAAACCTTTTCTTTGG *F CCCGCGGCCATGAAATCGAACGCGACAATTACCTGAGCTctctaaagacactcccgaatcgggagaccgtaacgaagctt *F gctgccacagagaggagatcatccacaatagataattggcgtcagccaattgattaccaaagcgaaagagatttgcatca *F gaaaaacaaccacagtaagtaccaataccaactcatatga *F Protein sequence: *F MVKDIPDRWLKYKPIGDRVPGTRFIAFKVPLNQHVNAKVKENLRLAPESLLQIVPDMGLIIDLTNTNRYYHPSAITNHDV *F LHQKLMIPGKQTPSHKLAQRFCAFVTDFLERNADNDKLIGVHCTHGVNRTGYLICYFMISVMNMSPEEAIQTFSLARGHE *F IERDNYLSSLKTLPNRETVTKLAATERRSSTIDNWRQPIDYQSERDLHQKNNHSKYQYQLI. *F Comments: \- ATG is at position 97 in cDNA sequence (5' end of EST clone *F RE27552) *F \- Two exons containing the COOH end of the protein were missed. There is *F experimental evidence for the existence of these two exons as they code for *F sequences present in EST clone RE27552.In addition, these sequences contain *F important motifs for the postulated PTPase function of the gene. *F \- There appears to be differences in the genomic sequences corresponding to *F CG13197 in AE003825 and AC011696: the exons of CG13197 are not in the same *F order in the two sequences. Based on the sequence of the EST clone RE27552, *F AC011696 appears to be correct, and the discrepancy is in AE003825. # *U FBrf0141827 *a Caggese *b C. *t 2001.12.11 *T personal communication to FlyBase *u FlyBase error report for CG1319 on Tue Dec 11 09:50:54 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 11 Dec 2001 09:50:54 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG1319 on Tue Dec 11 09:50:54 2001 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG1319 *F Release: 2 *F Gene annotation error *F Gene CG1319 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG1319 cDNA *F gtcacacacaacttcacgtttaacaaaatttaattgatttccttattatt *F taacaaccgatcgcagcgacacgagtgggtgcgtgttgtcagttatagta *F agagattgcaaaggaatcctaaccagaatgctagtcataaactcatgcag *F agctgcctccagattggcgctacgcagcctcaatttgcgatcgccgatcg *F caacgcgtactttctcgacgggattggccctgaaaacgaaagatgttgtt *F aacataaccttcgtgcgtgccaatggggacaagattaagacgtccggaaa *F agtgggtgactccctgctggacgtggtggtcaacaacaatgtggatctgg *F atggtttcggggcctgtgagggcacgctgacctgctccacctgccacctg *F atcttcaagaccagcgatttcgagaaactgcccgacaaacccggtgatga *F ggagctggacatgctggatttggcgtacgaactgacggacacctcgcggc *F tgggctgccagatcaccctgtccaaggatatggagggcctggaggtacat *F gtgccctccaccatcaatgacgcacgtgccgcgtagatagacaccaccac *F tgttatcaattgttacaaatttgagtgctaattcgctttagttcaaccta *F gatgtagtcaatatttttgaacgaaatcgtatgtattgtcccagggacac *F aaacc *F Protein sequence: *F >CG1319 gene product *F MLVINSCRAASRLALRSLNLRSPIATRTFSTGLALKTKDVVNITFVRANG *F DKIKTSGKVGDSLLDVVVNNNVDLDGFGACEGTLTCSTCHLIFKTSDFEK *F LPDKPGDEELDMLDLAYELTDTSRLGCQITLSKDMEGLEVHVPSTINDAR *F AA *F Comments: RH56686.5prime BI628320 *F RE29894.5prime BI234228 # *U FBrf0141828 *a Caggese *b C. *t 2001.12.10 *T personal communication to FlyBase *u FlyBase error report for CG3140 on Mon Dec 10 08:29:30 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 10 Dec 2001 08:29:31 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG3140 on Mon Dec 10 08:29:30 2001 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG3140 *F Release: 2 *F Gene annotation error *F Gene CG3140 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG3140_cDNA *F cagggtgtttcgtgtgaatgatttcgcctcttgtttcttatctttaacct *F tcatttctggctccgtttggctatgaattaggagcagtcccgaataatca *F gtcgagcatacgtagttaacgatggcgccgaatgcagcagtaccagtgga *F gcgatatgagcctgagaacatcgggatcaacgccattctcctggggccgc *F cgggttccggcaaaggaacgcaggctcccctgctgaaggagaagttctgc *F gtgtgccacctgtcgaccggcgacatgctgcgggcggagatttcatccgg *F ttcgaagctgggagccgagttgaagaaggtcatggatgccggcaaactgg *F tctccgacgacctggtcgtggacatgatcgactccaacctggacaagccc *F gagtgcaagaatggattcctcctggacggattccccaggactgtcgttca *F agctgagaagctcgacacattgctggacaagaggaaaaccaacttggatg *F ctgtcatcgagtttgccatcgacgacagtctgttggtgaggcgcatcact *F ggccgcttgatccaccaggccagcgggcgttcgtatcacgaagagtttgc *F ccctccaaagaagccaatgacggacgatgtcactggagagcccctaatcc *F gtcgcagcgatgacaacgccgaggctctgaagaagcgactggaagcatat *F cacaagcagacaaagccgctggtcgactactacggcctgaggggtctgca *F cttcaaggtggacgccgccaagaagtccagcgacgtcttctccaccatcg *F actctatattccaacgcaagcgtcctgcccagatccagttatgatttgtt *F cctgcgcaatgatcaactactgataactgcctcctgacaatttatttcga *F gacacacacaacgtttaagcctaaagttatagttgcatccggaaatcctg *F aacaaacgaaattacctttgcaggtcgactactttccttgtacagatttc *F taaagtgtatgtgtttgttactttttacatttcactatttgtaactacaa *F tacaatttaataaaaaaatcgaaggaaacaagt *F Protein sequence: *F >CG3140 gene product *F MAPNAAVPVERYEPENIGINAILLGPPGSGKGTQAPLLKEKFCVCHLSTG *F DMLRAEISSGSKLGAELKKVMDAGKLVSDDLVVDMIDSNLDKPECKNGFL *F LDGFPRTVVQAEKLDTLLDKRKTNLDAVIEFAIDDSLLVRRITGRLIHQA *F SGRSYHEEFAPPKKPMTDDVTGEPLIRRSDDNAEALKKRLEAYHKQTKPL *F VDYYGLRGLHFKVDAAKKSSDVFSTIDSIFQRKRPAQIQL *F Comments: SD09634.5prime AI543051 *F RH09019.3prime BI573355 # *U FBrf0141829 *a Dow *b J.A.T. *t 2001.12.6 *T personal communication to FlyBase *u FlyBase error report for CG12286 and karmoisin on Thu Dec 6 07:17:59 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 6 Dec 2001 07:17:59 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: j.a.t.dow@bio.gla.ac.uk *F Subject: FlyBase error report for CG12286 and karmoisin on Thu Dec 6 07:17:59 *F 2001 *F Error report from Julian A.T. Dow (j.a.t.dow@bio.gla.ac.uk) *F Gene or accession: CG12286 and karmoisin *F Release: 2 *F Missed gene *F Comments: karmoisin is CG12286 *F karmoisin is a late-acting gene in the xanthommatin eye pigment pathway. *F It has tentatively been assigned a role as phenoxazizone synthetase, *F catalysing the bimolecular condensation of 3-hydroxykynurenine to the pigment *F xanthommatin: *F see gene report for kar: *F 'Phenoxazinone synthetase level subnormal in mutants; maybe a structural gene *F for this enzyme; 3-hydroxykynurenine accumulates (Sullivan, Kitos and *F Sullivan, 1974).' *F However, the only Blast matches for Streptomyces antibioticus Phenoxazizone *F synthase (AAA86668) are multicopper oxidases at 16D6 and 96F8. *F In cloning and rescuing Su(fu), Pham et al., 1995 *F ('The suppressor of fused gene encodes a novel PEST protein involved in *F Drosophila segment polarity establishment. Genetics 140(2): 587--598 *F FBrf0082477') showed that kar is rescued by the 4kb immediately downstream *F of Su(fu), and provisionally worked out some of the exons of kar, which was in *F the same orientation. *F With the benefit of the genome project, we can see that the only ORF and EST *F hit in this region is CG12286. This has been identified by Gadfly and my *F searches as a monocarboylic acid transporter (solute carrier family 16). *F I believe the work by Pham *F (http://fly.ebi.ac.uk:7081/.bin/molmap.html?FBgn0001296) is sufficient to *F identify this gene unambiguously. *F Interestingly, 3-hydroxykynurenine IS a monocarboxylic acid *F (http://www.genome.ad.jp/dbget-bin/www_bget?compound+C03277). *F I therefore propose that kar is the transporter responsible for getting *F 3-hydroxykynurenine to its target site, where another enzyme catalyses the *F condensation to xanthommatin. *F Consistent with this, CG12286 is very heavily represented in the RH (head) EST *F sequences released in August 2001. *F Does kar act to import 3-hydroxykynurenine at the plasma membrane, or into *F vesicles? A Psort II prediction with the Gadfly predicted peptide suggests *F that this is plasma membrane: *F >k = 9/23 *F > 69.6 %: plasma membrane *F > 21.7 %: endoplasmic reticulum *F > 4.3 %: mitochondrial *F > 4.3 %: nuclear *F > *F >>> prediction for QUERY is pla (k=23) # *U FBrf0141830 *a Schupbach *b T. *t 2001.11.15 *T personal communication to FlyBase *u *F From: 'Schupbach, Gertrud' *F To: ''Leyla Bayraktaroglu'' *F Subject: RE: a grk allele question *F Date: Thu, 15 Nov 2001 20:18:37 \-0500 *F Dear Leyla, *F I just checked something on the flybase website under the gurken entry and *F noticed that there was another confusion \- which my lab is responsible for *F as well. The allele 2B6 is the same as 2B, i.e. 2B is a synonym. Some *F people in my lab have dropped the '6', and I did not notice this *F inconsistency in the manuscripts, because by now I am so used to either of *F the names. However, since we define 2B6 molecularly, we should use that *F name, and not 2B. Do you think you could change the fly base entry *F accordingly and list 2B just as synonym for 2B6? *F Sorry for this. *F All the best *F Trudi # *U FBrf0141831 *a Weiss *b J. *t 2002.1.2 *T personal communication to FlyBase *u *F Date: Wed, 02 Jan 2002 13:29:54 \-0800 *F From: 'Joseph Weiss' *F To: *F Dear Rachel, *F Thanks for your note. I have not received any queries about the P-element *F insertion into jelly belly, perhaps because I moved recently to Oregon. In *F any case, you're right, it is l(2)k05644. I hope that resolves the *F uncertainty and if you have any other questions please don't hesitate to *F contact me, only too happy to oblige. *F Best wishes for the New Year, *F Joe *F Joseph B. Weiss MD PhD *F Assistant Professor of Molecular Medicine and Cardiology *F OHSU *F 3181 SW Sam Jackson Rd. NRC3 *F Portland, OR 97201-3098 *F Office phone: (503) 494-6412 *F Lab phone: (503) 494-6855 *F Fax: (503) 494-7368 *F E-mail: weissjo@ohsu.edu *F >>> Rachel Drysdale (Genetics) \- 1/2/02 3:25 AM >>> *F Dear Joe, *F FlyBase does have a gene record for jelly-belly (FBgn0033681) in the *F master genes file, however due to updating-timing issues this is not *F yet evident on the public servers. .. *F Your paper (Cell 107(3) 387--398) has been curated by Gillian Millburn *F however the curation record has not yet been integrated into the main *F FlyBase dataset. Gillian is out of the office until next week, but *F from her curation record I can surmise that she is waiting for a reply *F to a query about the identity of an unnamed P-element in Figure 2A, which *F might be l(2)k05644. *F When this is resolved the curation record will be released and the new *F data will (without you having to do anything else) augment the *F jelly-belly record below. Also, since you unambiguously refer to the *F gene as jeb in your Cell paper its valid symbol will become jeb and *F jelly-belly will be its full name. *F Hope this explanation helps, *F Regards, *F Rachel. *F FlyBase Consortium. *F > From gmpan1@attbi.com Wed Dec 26 04:59:47 2001 *F > Date: Tue, 25 Dec 2001 20:59:19 \-0800 *F > Subject: jelly belly *F > To: *F > *F > Dear Flybase, *F > *F > I have been checking for the Jelly Belly (Jeb) entry since we published the *F > paper in Cell. Nothing yet. If I have omitted some essential ritual, I *F > apologize, but I assumed that publication was sufficient to ensure notice by *F > Flybase. Is there something I need to do to garner a Flybase entry? *F > *F > If I can help with the entry please feel free to contact me at work: *F > weissjo@ohsu.edu. *F > *F > Thanks, *F > *F > Joe Weiss *F > *F > # *U FBrf0141832 *a Ruden *b D. *t 2002.1.7 *T personal communication to FlyBase *u FlyBase error report for CG4578 on Mon Jan 7 08:36:30 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 7 Jan 2002 08:36:31 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: douglasr@uab.edu *F Subject: FlyBase error report for CG4578 on Mon Jan 7 08:36:30 2002 *F Error report from Douglas Ruden (douglasr@uab.edu) *F Gene or accession: CG4578 *F Release: 2 *F cDNA or EST error *F Comments: Please add cnc-C as a third alternative splice. Also, add EP3633 and *F EP3258 as insertions into the 5' UTR of cnc-C. # *U FBrf0141833 *a Jiang *b L. *c S. *d Crews *t 2002.1.5 *T personal communication to FlyBase *u *F From: 'Stephen Crews' *F To: 'Beverley Matthews' *F Subject: RE: update question *F Date: Sat, 5 Jan 2002 16:50:39 \-0500 *F Beverley, *F Attached is a Word file containing the cDNA sequence of cranky with splice *F sites, ORF, and terminator codon listed (fastA format sequence also included *F at bottom). The sequence is from the genomic sequence AC008210, and not the *F cDNA clone sequence, which was derived by RT-PCR from y w embryos \- there *F were no substantial differences. It is likely that both the 5'-end and *F 3'-end of the sequence are shorter than the full-length mRNA. In *F particular, when we primed cDNA with oligo(dT), we seemed to be priming from *F an internal genomic poly(A) stretch. 5'-RACE has not been successful. *F As you will see, cranky includes CG14554, CG12561 exon 2 (but not 1 and 3), *F CG14553 exon 2 (but not 1 and 3), and CG14552 exons 1-4. Cranky exons 3-4 *F were not predicted by Genie. *F .. *F Steve *F \------------------------------------------------ *F Stephen Crews *F Professor *F The University of North Carolina at Chapel Hill *F Department of Biochemistry/PMBB *F CB#3280 Fordham Hall *F Chapel Hill, NC 27599-3280 *F (919) 962-4380 (Tel) *F (919) 962-8472 (Fax) *F steve_crews@unc.edu *F http://www.unc.edu/~crews *F \------------------------------------------------------------------------------- *F LOCUS Cranky cDNA 2710 bp DNA 5-JAN-2002 *F COMMENTS 5'-end and 3'-ends are likely to be incomplete (oligo(dT)-primed *F cDNA seems to be primed from genomic poly(A) stretch); position of initiator *F methionine (if present) is unknown. The sequence below is derived from (and *F identical to) genomic sequence AC008210. *F ORGANISM Drosophila melanogaster *F COMMENT AUTHORNAME|Lan Jiang, Stephen Crews| *F COMMENT AUTHORTEL|919-962-4380| *F COMMENT AUTHOREML|steve_crews@unc.edu| *F COMMENT AUTHORAD1|UNC-Chapel Hill| *F FEATURES Location/Qualifiers *F exon 1..357 *F exon 358..539 *F exon 540..610 *F exon 611..700 *F exon 701..1000 *F exon 1001..1185 *F exon 1186..1292 *F exon 1293..1446 *F exon 1447..2710 *F terminator 2660..2662 *F ORF 2..2659 *F BASE COUNT 707 a 812 c 642 g 549 t *F ORIGIN *F 1 acgtgcgatc gacgatatgt atgcggataa taaacgtggc ctagacaagg tgacggcagc *F 61 aaccccgccc acccacgccc tccagcagca gcagcagcag caacatcccc accagcagca *F 121 gcagcatctg cagcatgtcc tgcaactgca gcagcaacag cagcaacacc ttcagcagca *F 181 gcaacatcac ttgcagcacc agatgcatac gcatcataac ttccagcagc atgaaacgtc *F 241 gccaacagtg tcgcagcagc agcacatgca acagcagcag ccgcagacga cacaacagca *F 301 acatgcaaca cagcaacagc agcagcaaca gcaacagaca gccggcatgt ttacaagatt *F 361 cgatgcaaac aaatcgacga agggcgcctc gaagatgcga cgcgatctca taaatgcgga *F 421 gatagccaat ctgagggatc tcctgccgct gccccaatcg acgcgccagc gcctctcgca *F 481 actgcaactg atggccctcg tttgtgtcta tgtgcggaaa gccaactact tccagcaagt *F 541 tttcaaacgt cacaatgcga tgcatcatct ccatcatcag acagcaacac caaatattgg *F 601 cttctcaaag gcactctccg gcttcttgat gatgctcaca caaaatggca aattgcttta *F 661 tatatcggat aatgctgcgg agtacttggg ccattccatg gaggatctgc tgatacacgg *F 721 tgactccgtt tacgatatca ttgacaaaca ggatcacgca aacatccagg ccgagctgaa *F 781 taggaacgtg ccaccgcagc cgggtggctc gagtcaggcg agcagcggtg gtgccggtgg *F 841 tgctggcggt gccggcgact caacgtccag caatggaagt gccgctggtg ccggtggtgc *F 901 tggtggcgcc tccagtctcg agggcgagca tcgcatgttc ctctgtcgca tgaatgttag *F 961 ccgcaatgcg cgacggcaaa tgcgtttcgg cgatcagaag gttgtcttgg tccagggtca *F 1021 ctatttgtca ttcctgccgc tttgcagtcg caacgagccc gtctttttgg ccacctgcac *F 1081 tccgattgcc atgcccgaaa ccagggagtg tgttgtacaa ggtgccacca acgtcttcac *F 1141 caccatccat tcaatggaca tgaaaatagc ccatatcgat aaaaatggcg aatttcattt *F 1201 gggctatgat aagtcgactc tgcaaggcac atcctggtac aatctgatcc acagcgataa *F 1261 tctgacggag gcgcaaaaaa aacacagttt aattatccaa tcggaacaag atcgcagttg *F 1321 tatccttcta gtacgaatgc agagatctag tggggaatac acgtgggtac acgtggtttt *F 1381 acaagtgcga gatagtcccg actccacgca acaacctgtt atcgtgtgca caaaccaagt *F 1441 actcaacgat cgagaggcct caattatgct ggccaactcc tggctgtatc actactatac *F 1501 cgtccagtcg aagatccagt tcggcatgcc gctggacagt ggtatccgtg tcccaccggg *F 1561 aactccctcc agcggctact acagtcccca ttcatcccat ccaccggcca ctccaggtgg *F 1621 tgcaacaccc aatttgggca tagccagtag ctttggcccg caccatcatc cacaccactc *F 1681 gcaccacccg caccaccatc atcaccatca ccatccggcg actgcttacc atcaccatcc *F 1741 gcatatggga acctatggtg gagtctatca tgcgaaggca gcaatcgagc ttaaggagga *F 1801 tcaaccgctg ttttgctttc aagagcccgt ggactatagc caagtaaata gccacgttaa *F 1861 tcctccgaat gcaacaagta ctcccaatcc gcctatctcc aaatcgtcca cgcccccacc *F 1921 gcccaagaag cgggctcgaa aactggaacc actctactta cacgccgaac gctctccggc *F 1981 ggcggcgata actccggaac cggattgcta tgccatccat actcatccgg ctgctgccta *F 2041 tgccacctca tcggtggtca gtagtgatgc ttcagttata tatgccacaa tagtgccgac *F 2101 gagaccgagg ttacccaata aatcactgcc tccggatcct gcggatttta tagagcaatg *F 2161 gaaccccagt ccaccctggt cggaatcggc gcaaaaggct tcgttggata gttttggatc *F 2221 ctcccacgag ctgagtccct gcatcacgac cacgcccccc acgcccacaa gtgctacacc *F 2281 gcatcaaagt ggcttgggca ccacccagac cataggacct gctttcagtt ttgaatggat *F 2341 gtcagatcct ttggtaccgg tcacgtacca gcagtggcca ccaactggtg accatcatca *F 2401 gcatctttcg cagcacttaa gccagagtca aaaccaccaa aatcccctcc agcagcagca *F 2461 gaacgagcag cttctacttg gacaacagcc gcatctccac cagcagcacc agcacttagc *F 2521 acttcatcat ggacgtgggg agcactccct ggagggggaa agtcaggggg tggtggtagt *F 2581 gccaccgata cccatttcga taccgatacc aacggccacg ggccatgggg atgccggcga *F 2641 accggttgag gatagaggtt agtattaagt gtgtattgtt ttttaagttt ctggcacgat *F 2701 ttccaaaaaa *F // *F fastA *F >cranky.cDNA *F acgtgcgatcgacgatatgtatgcggataataaacgtggcctagacaaggtgacggcagcaaccccgccc *F acccacgccctccagcagcagcagcagcagcaacatccccaccagcagcagcagcatctgcagcatgtcc *F tgcaactgcagcagcaacagcagcaacaccttcagcagcagcaacatcacttgcagcaccagatgcatac *F gcatcataacttccagcagcatgaaacgtcgccaacagtgtcgcagcagcagcacatgcaacagcagcag *F ccgcagacgacacaacagcaacatgcaacacagcaacagcagcagcaacagcaacagacagccggcatgt *F ttacaagattcgatgcaaacaaatcgacgaagggcgcctcgaagatgcgacgcgatctcataaatgcgga *F gatagccaatctgagggatctcctgccgctgccccaatcgacgcgccagcgcctctcgcaactgcaactg *F atggccctcgtttgtgtctatgtgcggaaagccaactacttccagcaagttttcaaacgtcacaatgcga *F tgcatcatctccatcatcagacagcaacaccaaatattggcttctcaaaggcactctccggcttcttgat *F gatgctcacacaaaatggcaaattgctttatatatcggataatgctgcggagtacttgggccattccatg *F gaggatctgctgatacacggtgactccgtttacgatatcattgacaaacaggatcacgcaaacatccagg *F ccgagctgaataggaacgtgccaccgcagccgggtggctcgagtcaggcgagcagcggtggtgccggtgg *F tgctggcggtgccggcgactcaacgtccagcaatggaagtgccgctggtgccggtggtgctggtggcgcc *F tccagtctcgagggcgagcatcgcatgttcctctgtcgcatgaatgttagccgcaatgcgcgacggcaaa *F tgcgtttcggcgatcagaaggttgtcttggtccagggtcactatttgtcattcctgccgctttgcagtcg *F caacgagcccgtctttttggccacctgcactccgattgccatgcccgaaaccagggagtgtgttgtacaa *F ggtgccaccaacgtcttcaccaccatccattcaatggacatgaaaatagcccatatcgataaaaatggcg *F aatttcatttgggctatgataagtcgactctgcaaggcacatcctggtacaatctgatccacagcgataa *F tctgacggaggcgcaaaaaaaacacagtttaattatccaatcggaacaagatcgcagttgtatccttcta *F gtacgaatgcagagatctagtggggaatacacgtgggtacacgtggttttacaagtgcgagatagtcccg *F actccacgcaacaacctgttatcgtgtgcacaaaccaagtactcaacgatcgagaggcctcaattatgct *F ggccaactcctggctgtatcactactataccgtccagtcgaagatccagttcggcatgccgctggacagt *F ggtatccgtgtcccaccgggaactccctccagcggctactacagtccccattcatcccatccaccggcca *F ctccaggtggtgcaacacccaatttgggcatagccagtagctttggcccgcaccatcatccacaccactc *F gcaccacccgcaccaccatcatcaccatcaccatccggcgactgcttaccatcaccatccgcatatggga *F acctatggtggagtctatcatgcgaaggcagcaatcgagcttaaggaggatcaaccgctgttttgctttc *F aagagcccgtggactatagccaagtaaatagccacgttaatcctccgaatgcaacaagtactcccaatcc *F gcctatctccaaatcgtccacgcccccaccgcccaagaagcgggctcgaaaactggaaccactctactta *F cacgccgaacgctctccggcggcggcgataactccggaaccggattgctatgccatccatactcatccgg *F ctgctgcctatgccacctcatcggtggtcagtagtgatgcttcagttatatatgccacaatagtgccgac *F gagaccgaggttacccaataaatcactgcctccggatcctgcggattttatagagcaatggaaccccagt *F ccaccctggtcggaatcggcgcaaaaggcttcgttggatagttttggatcctcccacgagctgagtccct *F gcatcacgaccacgccccccacgcccacaagtgctacaccgcatcaaagtggcttgggcaccacccagac *F cataggacctgctttcagttttgaatggatgtcagatcctttggtaccggtcacgtaccagcagtggcca *F ccaactggtgaccatcatcagcatctttcgcagcacttaagccagagtcaaaaccaccaaaatcccctcc *F agcagcagcagaacgagcagcttctacttggacaacagccgcatctccaccagcagcaccagcacttagc *F acttcatcatggacgtggggagcactccctggagggggaaagtcagggggtggtggtagtgccaccgata *F cccatttcgataccgataccaacggccacgggccatggggatgccggcgaaccggttgaggatagaggtt *F agtattaagtgtgtattgttttttaagtttctggcacgatttccaaaaaa *F \------------------------------------------------------------------------------- # *U FBrf0141834 *a Levis *b R. *t 2002.1.3 *T personal communication to FlyBase *u FlyBase error report for CG17131 on Thu Jan 3 13:05:38 2002. *F Date: Thu, 3 Jan 2002 17:26:43 \-0500 *F To: bdgp@fruitfly.BDGP.berkeley.edu *F From: Robert Levis *F Subject: Correction for FlyBase error report for CG17131 on Thu Jan 3 *F 13:05:38 2002 *F Cc: flybase-updates@morgan.harvard.edu *F I recently sent an error report for CG17131 using the GadFly Fix *F annotations form. A copy of the email confirmation is copied below. *F I realized after I sent in this error report that I gave the wrong *F coordinates for the 5' exon of the SP71 mRNA in my error report. The *F untranslated 5' exon maps at 26021-25703 of AE002567.1. *F >Error report from Robert Levis (levis@ciwemb.edu) *F >Gene or accession: CG17131 *F >Release: 1 *F >Missed gene *F >Comments: The GenBank annotation for CG17131 (= SP71) places the 5' *F >end of the gene and mRNA at position 5358 of scaffold segment *F >AE002567.1. The mRNA sequence of SP71 submitted by Selano et al. *F >(AF212322.1) has an untranslated exon mapping 20 kb upstream on the *F >genomic sequence, at positions 25722-25703. *F > *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0141835 *a Levis *b R. *t 2002.1.3 *T personal communication to FlyBase *u *F Date: Thu, 3 Jan 2002 16:51:21 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: Flanking sequence for P{lacW}G0280a \- Correction *F A few minutes ago I sent a message regarding the flanking sequence *F for P{lacW}G0280a. The message contained an error in the distance of *F this insertion from the 5' end of the SP71 gene mRNA. Here is a *F revised version of this communication, in its entirety: *F There is a GenBank record (gi:11071393) with the genomic sequence *F flanking the P element insertion P{lacW}G0280a (FlyBase ID: *F FBti0015480). This is an insertion in 1A5-6, which is one of two *F insertions in Bloomington stock 12291. The 5' end of this sequence, *F which corresponds to the beginning of the 8 bp target site *F duplication flanking the insertion, maps to genomic scaffold segment *F AE002567.1 position 26111. This is 90 bp upstream of the 5' end of *F the mRNA for the SP71 gene, according to the submission by Selano et *F al. (GenBank accession AF212322.1). *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0141837 *a Blair *b S. *t 2002.1.8 *T personal communication to FlyBase *u *F \------------------------------------------------------------------------------ *F Personal communication from Seth Blair, 8.1.2002. *F The gene referred to in FlyBase as 'acox' and described in: *F Conley et al., 1998, A. Dros. Res. Conf. 39: 159C *F Conley et al., 1999, A. Dros. Res. Conf. 40: 403C *F corresponds to the two acyl-Coenzyme A oxidase genes in 57D \- Acox57D-p *F and Acox57D-d, shown in Figure 5 of: *F Conley et al., 2000, Development 127(18): 3947--3959 *F The gene referred to as 'Agr-like' in Figure 5 of: *F Conley et al., 2000, Development 127(18): 3947--3959 *F corresponds to CG9822 (and the LP05143 EST). *F \------------------------------------------------------------------------------ # *U FBrf0141838 *a Weiss *b J. *t 2002.1.7 *T personal communication to FlyBase *u *F Date: Mon, 07 Jan 2002 10:29:24 \-0800 *F From: 'Joseph Weiss' *F To: *F Subject: Re: jelly belly *F Dear Gillian, *F We refer to the excision allele as jeb (superscript) \-c1. *F .. *F Thanks, *F Joe *F Joseph B. Weiss MD PhD *F Assistant Professor of Molecular Medicine and Cardiology *F OHSU *F 3181 SW Sam Jackson Rd. NRC3 *F Portland, OR 97201-3098 *F Office phone: (503) 494-6412 *F Lab phone: (503) 494-6855 *F Fax: (503) 494-7368 *F E-mail: weissjo@ohsu.edu *F >>> Gillian Millburn (Genetics) \- 1/7/02 7:00 AM >>> *F Dear Joe, *F .. *F I have just finished curating your Cell paper (Cell 107(3) 387--398) *F and have one additional question \- do you have a name for the 'P *F element excision derivative' allele of jeb that is described in the *F paper, so that I can put the phenotypes described under both the *F P-element allele (l(2)k05644) and the excision derivative allele (as *F you state that the phenotypes are indistinguishable). *F .. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0141839 *a Strub *b B. *t 2001.8.10 *T personal communication to FlyBase *u FlyBase error report for CG9242 on Thu Aug 9 19:02:20 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 9 Aug 2001 19:02:20 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: bstrub@yorku.ca *F Subject: FlyBase error report for CG9242 on Thu Aug 9 19:02:20 2001 *F Error report from Ben Strub (bstrub@yorku.ca) *F Gene or accession: CG9242 *F Release: 1 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG9242. *F Comments: The Drosophila gene burgundy (bur) was proposed to encode a GMP *F synthase gene, and its cytological position and sequence match that of CG9242. *F Because of this, and because there are no annotations with similar proposed *F function or sequence located in this region, I propose that burgundy and *F CG9242 are the same gene. # *U FBrf0141840 *a Levis *b R. *t 2001.7.24 *T personal communication to FlyBase *u FlyBase error report for CG17159 on Tue Jul 24 15:15:46 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 24 Jul 2001 15:15:47 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG17159 on Tue Jul 24 15:15:46 2001 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG17159 *F Release: 2 *F Missed gene *F Comments: CG17159 is probably in cytogenetic region 20E, since it is adjacent *F to the su(f) gene, which FlyBase estimates to be in cytogenetic region 20E. *F The annotated 5' end of CG17159 (13588 of AE002936.2) corresponds to the 5' *F end of an exon of unknown gene, adjacent to su(f) and divergently transcribed *F with respect to it, in the annotation of the sequence with accession X62679.1 *F (gi 2511641) submitted by O'Hare. While it is not stated, I infer that *F O'Hare's evidence for this exon is from analysis of cloned cDNAs. The *F annotated exon length in O'Hare's sequence is slightly longer than that *F predicted in the Celera/BDGP scaffold annotation. # *U FBrf0141841 *a Grishaeva *b T.M. *c S.Y. *d Dadashev *e Y.F. *f Bogdanov *t 2001.11.5 *T personal communication to FlyBase *u *F From grishaeva@vigg.ru Mon Nov 05 09:36:22 2001 *F To: *F Subject: Scientific information *F From: Grishaeva T.M., Dadashev S.Ya., Bogdanov Yu.F., Vavilov Institute of *F General Genetics, Gubkin St.3, 119991, Moscow, Russia *F Predicted gene CG17604 is c(3)G. *F We have shown by in silico analysis that the predicted Drosophila melanogaster *F gene CG17604 may be the known gene c(3)G, crossover suppressor on 3 of Gowen, *F causing gross reduction of crossing over and absence of synaptonemal complex *F (SC). CG17604 is located at position 36250-36253 kb on the NCBI molecular map. *F The related structural protein meets the requirements of the molecular *F structure of the protein of transverse SC filaments and fits parameters of *F D.melanogaster SC central space. It is a functional homologue of the known SC *F proteins \- Zip1p in yeast and SCP1 (SYCP1) in mammals. Our finding is in *F accordance with the experimental results from Scott Hawley' lab (Wayson et al, *F 17th European Research Conference, 1-5 September 2001, Edinburg) and his *F personal communication to us on September 23, 2001. Our finding is original in *F in silico identifying CG17604 as c(3)G. For details see: Bogdanov et al (2002) *F Russ.J.Genet. 38, \#1. # *U FBrf0141842 *a Bloomington Drosophila Stock Center *b ?. *t 2001.11.8 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Nov 08 17:27:38 2001 *F Subject: insertion phenotypes *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Kathy Matthews, Bloomington Drosophila Stock Center *F Subject: insertion phenotypes *F Dated: 8 November 2001 *F The following insertions are viable and fertile: *F P{FRT(whs)}G13 *F P{UAS-mCD8::GFP.L}LL5 # *U FBrf0141843 *a Kunes *b S. *t 2001.11.8 *T personal communication to FlyBase *u *F From kunes@fas.harvard.edu Thu Nov 08 15:30:30 2001 *F To: flybase-help@morgan.harvard.edu *F Hi \- *F .... Here is some additional info. unipolar disorder is equivalent to the *F gene l(2)k05916 and so should be updated along with that page. *F Thanks *F Sam Kunes *F Sam Kunes *F Room 433, Fairchild Building *F Harvard University *F 7 Divinity Avenue *F Cambridge, MA 02138 USA *F email: kunes@fas.harvard.edu *F telephone: 617-496-3806 *F fax: 617-495-8308 *F From rd120@gen.cam.ac.uk Thu Nov 08 15:43:34 2001 *F To: kunes@fas.harvard.edu *F Subject: upo and k05916 *F Hi Sam, *F to follow up on Aubrey's reply \- about merging l(2)k05916 with upo. *F The map data we have for upo places it at 53D (from Chung and Kunes, *F 1997, Dev. Biol. 186(2): 354). The map data we have for l(2)k05916 *F places it at 54B10--14 (BDGP \- by in situ hybridisation to P{lacW}). *F 54B10--14 is uncomfortably far from 53D, suggesting the possibility *F that the lethality of l(2)k05916 is what corresponds to upo (though we *F have no data at the moment that suggests upo mutants are lethal), *F rather than the P{lacW} insertion. Can you shed any light on this for *F us? *F Thanks for your help, *F Rachel. *F FlyBase Consortium. *F From kunes@fas.harvard.edu Thu Nov 08 18:23:05 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: upo and k05916 *F I would have to look back at the map data for upo, but I do know that *F l(2)k05916 is a noncomplementing allele of upo, so if there is generally *F high confidence in the BDGP P mapping, then we should go with that map *F position. How's that? *F Sam *F From rd120@gen.cam.ac.uk Fri Nov 09 10:46:23 2001 *F To: kunes@fas.harvard.edu *F Subject: Re: upo and k05916 *F Hi Sam, *F Thanks for getting back so quickly \- *F >but I do know that *F >l(2)k05916 is a noncomplementing allele of upo *F great \- do you also know for sure that the P{lacW} insertion is the *F cause of the noncomplementing phenotype? If so then I'm happy to merge *F and go with the BDGP mapping \- as the work progresses and upo gets *F placed onto the genome the map location will consolidate. *F Thanks, *F Rachel. *F From kunes@fas.harvard.edu Fri Nov 09 14:12:08 2001 *F To: Rachel Drysdale (Genetics) *F Subject: Re: upo and k05916 *F Yes, the P is non-complementing. # *U FBrf0141844 *a Guillemin *b K. *t 2001.11.15 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Nov 15 16:38:46 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: misguided *F The following information was provided by Karen Guillemin, University of *F Oregon (11/01). The locus l(2)25Ec (the l(2)jf27 locus of Szidonya and *F Reuter, 1988 (Genet. Res. 51: 197-208)) was renamed misguided. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141845 *a Levis *b R. *t 2001.11.16 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Fri Nov 16 18:27:38 2001 *F To: flybase-help@morgan.harvard.edu *F Subject: v(2)k05816 has a P insertion in CG3524 *F The flanking sequence of v(2)k05816 (Bloomington stock 10580) maps to *F scaffold AE003581.2 position ~216497. According to the GenBank *F annotation of this scaffold, this site is in the intron of the CG3524 *F gene. FlyBase currently lists no mutant alleles of CG3524; CG3524 *F and v(2)k05816 are listed as separate genes. While it is formally *F possible that v(2)k05816 is a separate gene from CG3524, located in *F the intron of v(2)k05816, it seems more likely that v(2)k05816 is an *F allele of CG3524. A caveat to this interpretation is the possibility *F of a double insert or a background mutation on the chromosome. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0141846 *a Bloomington Drosophila Stock Center *b ?. *t 2001.11.22 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Nov 22 19:36:32 2001 *F Subject: P{lacW}G0105 & l(1)G0105G0105 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Kathy Matthews, Bloomington Drosophila Stock Center *F Subject: P{lacW}G0105 & l(1)G0105G0105 *F Information communicated: *F l(1)G0105G0105 is separable from the insertion P{lacW}G0105. Once *F separated from the lethal the insertion has good viability and fertility in *F hemizygous males and homozygous females, and it shows an interesting *F pairing-dependent (apparently) regulation of the w+mC gene carried by *F the P{lacW} transposon. P{lacW}G0105 w-/FM7c females have *F moderate expression of w+mC (eye color is medium orange). *F P{lacW}G0105 w-/Y males and P{lacW}G0105 w- homozygous *F females have essentially no expression of w+mC. The addition of a Y *F chromosome to heterozygous females (P{lacW}G0105 w-/C(1;Y)3, *F In(1)FM7, w1 m2) has no obvious effect on w+mC expression. # *U FBrf0141847 *a Mahaffey *b J. *t 2001.11.27 *T personal communication to FlyBase *u *F From jim_mahaffey@ncsu.edu Tue Nov 27 19:56:24 2001 *F To: *F Subject: disconnected-related *F Just wanted to drop a line to tell you that there is no gene called *F disconnected-related. We called the gene disco-related, which is a synonym *F for CG9219. Maybe these records (disconnected-related and CG9219) could be *F fused together. *F Thanks, *F Jim Mahaffey # *U FBrf0141848 *a Stronach *b B. *t 2001.11.29 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Nov 29 18:44:06 2001 *F Subject: l(4)ry1 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Beth Stronach, University of Utah *F To: Bloomington Drosophila Stock Center *F Subject: l(4)ry1 complementation data *F Dated: June 1994 *F Information communicated: *F P{ry11}l(4)ry11 fails to complement Df(4)G # *U FBrf0141849 *a Luo *b L. *t 2001.12.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Dec 12 18:50:13 2001 *F To: Gillian Millburn (Genetics) , *F Rachel Drysdale (Genetics) , crosby@morgan.harvard.edu *F Subject: Luo insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Liqun Luo, Stanford University (12/01). *F The following transposon insertions are all homozygous viable and *F fertile. The chromosome bearing the insertion is given. *F P{UAS-RhoGAP1A-dsRNA}2 3 *F P{UAS-RhoGAP1A-dsRNA}3 X *F P{UAS-RhoGAP5A-dsRNA}4.1 2 *F P{UAS-RhoGAP5A-dsRNA}2.1 3 *F P{UAS-Graf-dsRNA}4 2 *F P{UAS-Graf-dsRNA}5.1 3 *F P{UAS-RhoGAP15B-dsRNA}1.1 3 *F P{UAS-RhoGAP15B-dsRNA}9.3 2 *F P{UAS-RhoGAP16F-dsRNA}6.3 2 *F P{UAS-RhoGAP16F-dsRNA}3.2 3 *F P{UAS-RhoGAP18B-dsRNA}5.1 X *F P{UAS-RhoGAP18B-dsRNA}3.1 2 *F P{UAS-RhoGAP19D-dsRNA}2.1 3 *F P{UAS-RhoGAP19D-dsRNA}3.1 2 *F P{UAS-CdGAPr-dsRNA}6.1 2 *F P{UAS-CdGAPr-dsRNA}3.1 3 *F P{UAS-RacGAP50C-dsRNA}1.2 2 *F P{UAS-RhoGAP54D-dsRNA}5.1 3 *F P{UAS-RhoGAP54D-dsRNA}4.2 2 *F P{UAS-RhoGAP68F-dsRNA}4.1 3 *F P{UAS-RhoGAP88C-dsRNA}4.1 2 *F P{UAS-RhoGAP92B-dsRNA}2.2 2 *F P{UAS-RhoGAP93B-dsRNA}4.1 X *F P{UAS-RhoGAP100F-dsRNA}6.2 2 *F P{UAS-RhoGAP100F-dsRNA}3.1 3 *F P{UAS-RhoGAP102A-dsRNA}3.1 3 *F P{UAS-RhoGAP102A-dsRNA}7.2 2 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141850 *a Adachi-Yamada *b T. *t 2001.12.13 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 13 02:22:11 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-bsk.A-Y} construct and insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Takashi Adachi-Yamada, Kobe University (12/01). *F P{UAS-bsk.A-Y} was constructed by cloning a bsk (D-JNK) cDNA into a pUAST *F vector. *F P{UAS-bsk.A-Y}1 is a homozygous viable and fertile second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141851 *a Ayyar *b S. *t 2001.12.20 *T personal communication to FlyBase *u *F From sa245@mole.bio.cam.ac.uk Tue Dec 18 16:14:02 2001 *F To: Rachel Drysdale *F Subject: PC to FlyBase *F Dear Rachel, *F I am writing to request you to make the following changes to the gene *F symbols for achintya and vismay: *F achintya: symbol 'achi' *F vismay: symbol 'vis' *F Thanks, *F Savita. *F Savita Ayyar, Research Scholar phone: 01223-333751 *F Department of Anatomy fax : 01223-333786 *F University of Cambridge, *F Cambridge CB2 3DY # *U FBrf0141852 *a Deal *b J. *c K. *d Cook *t 2001.12.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 20 19:51:22 2001 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC9 *F Isolation and characterization of Df(2L)BSC9 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Df(2L)BSC9 was isolated as a P transposase-induced male recombination event *F involving P{PZ}cup01355 and P{lacW}l(2)k13315k13315. The deletion was *F isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 *F cn1 bw1 females x dpov1 P{lacW}l(2)k13315k13315/P{PZ}cup01355 *F cn1; TMS, P{Delta2-3}99B/+ males. The deficiency chromosome retains the *F miniwhite marker from the P{lacW} element based on w+ eye color in a *F w1118 background. Polytene chromosome squashes showed the breakpoints *F 26F5-7;27A2-B2 (at least part of 27B2 remains as a faint band). The *F deletion fails to complement cortQW55. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141855 *a Lamka *b M. *t 2002.1.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jan 03 15:16:25 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GawB}332.3 *F The following information was provided by Michele Lamka, University of *F Virginia (1/02). *F P{GawB}332.3 (FBti0002742) is a homozygous viable and fertile, second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0141856 *a Kose *b H. *t 2002.1.4 *T personal communication to FlyBase *u *F From crosby@morgan.harvard.edu Fri Jan 04 18:10:33 2002 *F Subject: pc from Hiro Kose, Samuel cDNA sequence *F To: curators@morgan.harvard.edu *F Personal communication from Hiro Kose, 18 Dec. 2001: *F The attached is the cDNA sequence for samuel gene. *F I have one request. *F I noticed that in FlyBase 'SAM-motif ubiquitously expressed punctatedly *F localized protein' is shown as full name of samuel. But recent observations *F have casted some doubt about the expression pattern of the protein. Thus, I *F would like to use 'samuel' as full name of the gene and delete the phrase, *F 'SAM-motif ubiquitously expressed punctatedly localized protein', at this *F point. *F Thank you. *F Sincerely *F Hiro Kose *F CGCGGTATTATCAGTGATCGAAATTTTAGCGATCGGCAAGATCAACGAGCAATGATCGCGAGCGCAAAGCGCGCGT *F AAATAGAGAAAATATAAATCAAGAGGATTGATAAAAAAGCGTATAGAACGCAAATAAGAAATAAGGTGAAAAGTGT *F GAGTGTGGGGGCGTTGTGTAAATGAAAGATTAAAAAAGTTCGGAAAAAGTGCTTACGGCGGAAGCGCACTACATAA *F CCGTAACGGTATCAGTTTTGACAGAAGGAAAAACGGGCCGGAAAATGTCACAAATACAGCGCAACTAATCCAAGCT *F AAAAATAGTCCGCGGAGAGATAAAAAATCACTGTGGCATTTGTTCGTGGCTTTTCGCTTTTTTACAACAAACTCAC *F GTGTCTAACGACACTTTGACAACAGAGATACAAATCTGTTTTGGGAAGCTGGGCACGTGACCGCCAATTAATGAAT *F GAGACAGCACCTGAAAGCCAAGAAAGAGATGGGCGGAGAGTGCGAGCGAGATAAGTGATAAGGCCGCGAAGAATAA *F AATCCCGAATCTTATGCTAATTGAGCGACAGAAAGTGAAACGCAAAAAATCAAGTTTAAAAGTCAAAAGTCACAAG *F CTAAATTAAATAAACAATGCTTTAAAAGTTCAAAGCTACAAACCACACATTTTAATTGTGGAGAAAATTAAAAGCA *F AAGCTATAAAGTTGAAATTTAGCAAACGTAAATCATTTATAGCCAAATATAGGATATAACAAATAGAGAATATTGG *F TTACGCCTCATCAAAAGTTTATAAATTATATGATTCTAATAGTACATAATAAAGCTCAAAAGTAAACACTAAACAA *F ATAAATAAAATTAATCAAACAATACAATTGCATACAAGTTAACGTTAACAAAAACTTCACCTTTTTTCAAACGAAC *F GACAAATTGACAACACAATTAGTCAGTCTGTCACTTCACTTAGTTGGTGGGCCACAATCAACGTTGAAACAATCAA *F AAAGCCAGCAGAGCGAAAGGGACAGCCGCGGATCGCCAGAAAGAGAGGGCAAATCGAATCGGTTGACGACGGCGGA *F GGTTTGACAATATGTTGAGCCCCCATAATGAAGGAAATTGATACGGAGACAACAGTAGTATGGCCAGCCTGGTGTT *F ATTCCGGTAACGCACCCACCGACATACTGCAGCAGAAGCCAGCCAAGGCCACCACCGCCATTGTGGGCGGTCGCAG *F CGCAGCCCAACCGGCGACCCACGACGACCAGGAAGCGGCCATGACCGTTTCCACAGCCACCAAGCGGAAGCATCTC *F GAGCGGTTGGCCAGCGATCTGCGGCAGCTGGGCAAGAAGGGCAGGAGGAGTCGATCGGTTAGTCCCTGTAGTAGAT *F ATCCCGCCGTTGCGGAAACGGCCAGAATGCTTAGCGAACGATCGCTGCTGCTCAGCGGATATGGAGCCATTGAGAG *F CAGTGCCAAGAAGCTGAGCGAAGCGGCGACCGGTGGTGCGGGTGGTGGTGGTGGTGGTGCGGCCGGTCAGGCTGCC *F ACGCCCACTACCAGCCAACTAAGTGCACCCGCCTCCTCAGCATCCTCCTCATCCATGTCGTCCTCCTCTGCCGGCA *F GCACTTGCACCACCAATGCAAATACGGCATCGGTGGCAGCCGCCTATGCTGCTTTCTATTTGGAGAAGGTCAAGCA *F TGAGAAGATGGACAATCATAAGGATGCACCGATGGTCACCATACACAATAACAACAACAACAACACCATCAACAAC *F AACAACAGCAGTAGCAGCAGCATCATCAACAACCATCAATCGAGTCAACCAAGTCTGGGCCACAGCGTCAAGAGGG *F AGCGGCTGAGTCCAGGGAGCAATAGCAGTAGCCACAACGGCGAACTCACCGTCAGCTCATTTGCCAGATCTCGCAG *F CGCCACGCCCTCGTCGTCATCGTATCGTGGTGCCGGCGGTGGTGCTGGTGGTGGTGTCTCACCCAGCCACCAGCAG *F CAGCAACAACAACAACAACAGTCCCTGCAGCAGCAACAACGCCTAAGTCCGCCCAGTGGCCCATCGATGGCAACAG *F CAACCACTACATCATCATTATCATCATCATCATCTGCATCGGCATCGGCATCGGCATCAGCAAGTGCAGCCACACA *F CATGCCGCTGCACAATTTGTCAACGAATTTGCTAAATAGCATTCAGCAGGCTGCCGCCAGTCAGCAGCAACTGCGC *F AGCAGCAACAGCAACAGCAGCAGCAACATCAGCGGCAGCAGCAACAACACCAAATCGGATGGTGGTGCCGCATCGA *F ATGCCACGGATTTCTCCACCCGCAACTATTCGGACATTATGCGATGCCTGGCGGCCAAGTACAACAATGCCAATCC *F CAATGACAATAACGCAACTCGACGCAATTCCTACTATGAAGGCAGCACTTCTGCCACCACAGCCACTGGAGGAGCC *F GGCGGTGGAGGTGGAGGAGCAGGAGGATCAAACTCTGCCCCAAGCAGCGCCCTCAAGTCGGCTAGCAGCTCCACCG *F GCAGCACTACGACCACTGCCAAGAAGCTCTCTCCATCGGCTGCATCGGCGGCGAACAGTGCTGCCGTCAGTCTGCC *F CAAGGAGTCCAAAGTGAGTGCGGTCGGTGGTGCAGGTGTGGGTGGTGCAGTAGCCAGTAGTGGTGGCGGTGGTTCG *F TCCATGTCGCAACTGGCTCCACCACCCACCGCTCCATCGCCCACGGAGCAGGCCAAGAGCCAAATGGCCGCCGTGG *F TGGCAGCAGCAAGTGTTTCGCCGTTTATGACCAACTTTCTGCCCTTCTCGTCTGGCATTTTCCCGCCGCTGATAGA *F CATGAGCAGCACCCAGGCACTGCTGACACTGGCGCGTGCTGTGAAAGATGCCGAAATCCAGGAGATTCTGCGCAGC *F AAACAGCAGCGCTCCGGTTCGAATGCCTCGTCGCCGAGTGCGAGTGCGGCGGGCACGCCACGTTCCAGTTCCACCC *F TGAATGCGGCACTGCATCAGGCAGCCCAATTTGTAACACCCGCTCTGATCTACTCGGCCCAGTTGCAACAGCAACA *F ACAGCAGCAGCAGCAGCAACATCAACGGCAGCAGCAGCAGCAATCGCAGCACGCTTCGCGGCAGTCTAGTCCACGT *F TCCACCAATGATTCCACGACGGTGGCGCCCATCGGTGACGGCGGCGGTGGCTCCTCGATTGCGTCGGCGGCGCCAT *F TGGATTTGAGCTCCCAGCCGCCGGCCGCCAAGCGCTTCAAGGCAGAGCGTCGTGCCAGCTCTACGGCCACCACAGC *F AACAACAGCCACCACAGGCTCATCCACGTCCACACCATCCCCGCCACCGTTGGAGCAGCATGACGATATCAATTCT *F GGCAACGCCAACGCGACACTAGGCACCGGATCGGGAACGGGAACGGGTGAGAGTTCTGCAGCGGGATCGGGAGCAG *F GAGTTGCCAAGGCCCGAAGACGATGTCAGGCGCAGAGCGAGGAGGTTAACTCCTGGTCTGTGGACGATGTGTGCGG *F CTTTGTGGGCGGAATTGATATATGCGCCGAATACGTTCAGTCGTTTCGTGATCAGTCCATCGATGGCACTGGCCTG *F CCGCTCTTAACCGAGGATCACCTGGTTAACTCGCTGGGCATGAAGCTGGGGCCGGCTCTCAAGCTGCGCTCCATAT *F TGGCGAAGAAATTGGGCGGGCCGTGTCCGTGTGTTGCATGCGTTGCGCAGGCGCAACAAATGCTGGCACTGCAAAC *F GGGTGGGGCAGCAGCAGCAACGGGAGCAACAGCAGGAGCAGGAACGGTTACGGGAGCGGTAGCGGGAGCGGGAGCA *F ACATCGGCCACAACTAGTTGCAGCATCAAGTCGGAGATCTTCAATGGCGGCAGCAACGGTAGCAGCAGCGGCAGCA *F GCAGCAACCAGGGCAGCGATGTTGCAGCTCCGCCGGCGGCAACAGCCAGCTCGCCCAGCAGCAACATGTTGCTGCC *F AGTGTTGCCGTGCAGCGGACTGCAGGACGCCAGCTAGTTAGCAGCGATCCTGTCCACATACGTATTTATGCTAACT *F AAATTGCATTTCACCTGCTAAAATGCTGAACCAAATGAGCTTTCAACACAATTAAGAAAGGAATAAGAGAATAAAAAAT *F AGACAGGCAGACAGGCGCCTCGGATTGGGAGAGAAATACCTATTGTATAAAAAAAAAAAAAAAAA *F AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAATTC # *U FBrf0144464 *a Ott *b S.R. *t 2001.12.13 *T personal communication to FlyBase *u FlyBase error report for CG3597 on Thu Dec 13 11:55:35 2001. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 13 Dec 2001 11:55:35 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: S.R.Ott@qmul.ac.uk *F Subject: FlyBase error report for CG3597 on Thu Dec 13 11:55:35 2001 *F Error report from Swidbert R. Ott (S.R.Ott@qmul.ac.uk) *F Gene or accession: CG3597 *F Release: 2 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG3597. *F Comments: The CG3597 gene product has been annotated as similar to Homo *F sapiens 'biliverdin reductase A' and therefore given the synonym BVRA by *F Dunkov, 2001.8.25 FBrf0138583. *F However, sequence analysis suggests that it is highly unlikely that the CG3597 *F gene product is a 'biliverdin reductase A.' Here are the reasons for my *F conclusion: *F 1) Protein-protein BLASTing the CG3597 gene product against Entrez Genome *F reveals highly significant similarity to vertebrate 'dimeric dihydrodiol *F dehydrogenases' (E-values between 3e-59 and 5e-52) but not a single *F 'biliverdin reductase A' within the first 100 hits (up to 7e-07). *F 2) Pairwise protein-protein BLAST between *F 'dimeric dihydrodiol dehydrogenase; 3-deoxyglucosone reductase Homo sapiens' *F gi:7657212 (Length: 334 AA) *F and *F 'CG3579 gene product' gi:7295965 (Length: 335 AA) *F gives an E-value of 5e-53 *F and a positive alignment region stretching over 98% of CG3597 *F [Score = 208 bits (530), Expect = 5e-53, Identities = 124/329 (37%), Positives *F = 177/329 (53%), Gaps = 4/329 (1%)] *F 3) Pairwise protein-protein BLAST between *F 'biliverdin reductase (EC 1.3.1.24) \- human' gi:7512304 Length: 296 AA) *F and *F 'CG3579 gene product' gi:7295965 (Length: 335 AA) *F gives an E-value of 26 *F and a positive alignment region of only 18% of CG3597 *F [Score = 30.0 bits (66), Expect = 26, Identities = 19/59 (32%), Positives = *F 27/59 (45%), Gaps = 1/59 (1%)] *F 1--3 clearly shows that CG3597 should be re-classified as a putative 'dimeric *F dihydrodiol dehydrogenase' *F 4) There is a very plausible explanation for the previous classification as *F 'biliverdin reductase A': When the Drosophila genome is BLAST-searched for *F proteins that are similar to vertebrate biliverdin reductase, the 'CG3597 *F gene product' in question (E-value = 1.0) indeed shows the second-best *F alignment (after 'CG3609 gene product', E-value = 0.096). *F I therefore assume that such a search has been performed by Dunkov, 2001.8.25 *F FBrf0138583, in an attempt to identify Drosophila-orthologs of vertebrate *F bilirubin reductase A, and that this has lead to the erroneous classification *F of CG3597 as 'biliverdin reductase A' # *U FBrf0144465 *a Deal *b J. *c K. *d Cook *t 2002.1.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jan 04 23:47:29 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC10 *F Isolation and characterization of Df(3L)BSC10 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center *F Df(3L)BSC10 was isolated as a P transposase-induced male recombination *F event involving P{lacW}mirrcre2 and P{lacW}RpS12s2783. The deletion was *F isolated as a rhove-1-e1 recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{lacW}mirrcre2/P{lacW}RpS12s2783 e1 males. Polytene chromosome *F squashes showed the breakpoints 69D4-5;69F5-7. The deletion failed to *F complement Ptp69D1, Df(3L)iro-2 and Df(3L)eygC1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145552 *a Bourbon *b H.M. *t 2002.1.31 *T personal communication to FlyBase *u *F Date: Thu, 31 Jan 2002 08:06:03 \+0100 *F To: cy200@gen.cam.ac.uk *F From: henri-marc bourbon *F Subject: MOD paper *F Dear Chihiro, *F I am writing to you on behalf of Dr. Alain Vincent. Thank you for your *F watchfulness about our MOD paper. Please, find hereafter the current data *F about the following P insertions: *F (1) PL25 is inserted at position 126012 of GenBank AE003494. The nearby *F (and most likely affected) gene is CG18319/bendless. However, we also found *F in its immediate vicinity a short ORF encoding a putative mitochondrial *F protein that to date has not been annotated. *F (2) PL60#1 is inserted at position 77698 of AE003494. The nearby (and most *F likely affected) gene is Beadex. However, the P element is closed to the 3' *F region of CG15047. Is is also formally possible that the latter corresponds *F to an alternative 5'exon of Beadex. *F (3) PG80 and PG112 are inserted 234 pb apart (at positions 102458 and *F 102692 of AE003444, respectively) closed to the 3' end of both PIP82 and *F CG1521. Therefore, it was not possible for us to predict the affected gene. *F I hope these informations will answer to your questions. *F Best regards. *F Henri-Marc *F Dr. Henri-Marc Bourbon, phD *F Centre de Biologie du Développement *F UMR5547 du C.N.R.S. *F Université Paul Sabatier Toulouse III *F 118 Route de Narbonne *F 31062 Toulouse *F France *F e-mail: bourbon@cict.fr *F phone: (33) 05 61 55 82 88 *F Fax: (33) 05 61 55 65 07 # *U FBrf0145593 *a Whitfield *b E. *t 2002.1.10 *T personal communication to FlyBase *u *F Date: Thu, 10 Jan 2002 09:02:07 \+0000 *F From: Eleanor Whitfield *F To: cdna@fruitfly.bdgp.berkeley.edu *F CC: Eleanor Whitfield *F Hi, *F HL05775 full length cDNA is in TrEMBL entry: Q95RZ2 and is annotated as *F being norpA. *F I have done a BLAST of the DNA in the CDS feature annotated as being *F norpA (attached file) and find that it hits the genome project genomic *F sequence (superb!) but none of the other nucleotide entries that encode *F norpA: *F J03138; AAA28724, from Cell 54:723-733(1988) *F AF181641; AAD55427, another full length cDNA from your group. *F The annotation of norpA in the genomic sequence is wrong (please notice *F this bit Gillian/reannotation curators!!). The mRNA feature lists more *F exons that the CDS feature: *F FT mRNA join(254270..254602,254669..254729,258474..258630, *F FT 259488..259567,262057..262293,262362..262528, *F FT 262593..262897,263293..263457,263522..263888, *F FT 264084..265347,265412..265568,265633..265722, *F FT 266983..267067,267475..268913) *F . *F . *F FT CDS join(254423..254602,254669..254729,258474..258508) *F Consequently the current annotation translates a truncated protein but *F by adding the exons illustrated in the mRNA feature the translation *F would be correct. *F >From the BLAST, the cDNA maps to within the last exon annotated in the *F norpA mRNA feature so I suggest that this cDNA is not norpA but an *F adjacent new gene. Currently there is nothing in SWISS-PROT or TrEMBL *F (see fasta, saved for 24 hours only: *F http://www.ebi.ac.uk/servicestmp/999980.808874-375148.html) that is *F anything like this translation but annotation of the cDNA suggests it is *F real. *F Hope you can follow this, if not please feel free to ask, *F thanks *F Nellie *F \------------------------------------------------------------------------------ *F -- *F Header of the page *F BLASTN 2.2.1 Apr-13-2001 *F Reference: *F Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, *F Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), *F 'Gapped BLAST and PSI-BLAST: a new generation of protein database search *F programs', Nucleic Acids Res. 25:3389-3402. *F RID: 1010651965-2342-28632 *F Query= *F (258 letters) *F Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, *F GSS, or phase 0, 1 or 2 HTGS sequences). *F 1,079,316 sequences; 4,832,507,720 total letters *F If you have any problems or questions with the results of this search *F please refer to the BLAST FAQs *F Taxonomy reports *F Distribution of 10 Blast Hits on the Query Sequence *F Image *F \------------------------------------------------------------------------ *F Score E *F Sequences producing significant alignments: (bits) Value *F gi|17985874|gb|AC023742.3| Drosophila melanogaster X BAC RP... 511 e-143 *F gi|16768721|gb|AY061032.1| Drosophila melanogaster HL05775 ... 511 e-143 *F gi|10728458|gb|AE003430.2|AE003430 Drosophila melanogaster ... 511 e-143 *F gi|16924092|gb|AC026773.6| Homo sapiens chromosome 5 clone ... 42 0.24 *F gi|16924090|gb|AC026409.4| Homo sapiens chromosome 5 clone ... 42 0.24 *F gi|4938307|emb|AL049631.7|HSDJ513M9 Human DNA sequence from... 40 0.95 *F gi|2231346|gb|U78332.1|GPU78332 Galerella pulverulenta 12S ... 40 0.95 *F gi|15217430|ref|NC_003070.1| Arabidopsis thaliana chromosom... 38 3.7 *F gi|8778333|gb|AC007887.9|AC007887 Genomic sequence for Arab... 38 3.7 *F gi|3169212|gb|AC004773.1|AC004773 Homo sapiens PAC clone RP... 38 3.7 *F Alignments *F >gi|17985874|gb|AC023742.3| Drosophila melanogaster X BAC RP98-43C24 (Roswell *F Park Cancer Institute *F Drosophila BAC Library) complete sequence *F Length = 172198 *F Score = 511 bits (258), Expect = e-143 *F Identities = 258/258 (100%) *F Strand = Plus / Plus *F Query: 1 atgtgccctaaagatcgaaaacggagaagcgtttacactgtgctattaacgaatcgaaaa 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 17012 atgtgccctaaagatcgaaaacggagaagcgtttacactgtgctattaacgaatcgaaaa 17071 *F Query: 61 catttagttaactcatacaaggtaactgtactaaattatgtacaatatttaaccaacgcc 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 17072 catttagttaactcatacaaggtaactgtactaaattatgtacaatatttaaccaacgcc 17131 *F Query: 121 tacgactatgtatttagagaattgatcgagttttatgaatgtgataagcggttacatttt 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 17132 tacgactatgtatttagagaattgatcgagttttatgaatgtgataagcggttacatttt 17191 *F Query: 181 attacacgaacgcgagaaaaagcgaatcccaactaccctttatctccaatactttcgcct 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 17192 attacacgaacgcgagaaaaagcgaatcccaactaccctttatctccaatactttcgcct 17251 *F Query: 241 ttgccaacactgtcttaa 258 *F |||||||||||||||||| *F Sbjct: 17252 ttgccaacactgtcttaa 17269 *F >gi|16768721|gb|AY061032.1| Drosophila melanogaster HL05775 full length cDNA *F Length = 1890 *F Score = 511 bits (258), Expect = e-143 *F Identities = 258/258 (100%) *F Strand = Plus / Plus *F Query: 1 atgtgccctaaagatcgaaaacggagaagcgtttacactgtgctattaacgaatcgaaaa 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 544 atgtgccctaaagatcgaaaacggagaagcgtttacactgtgctattaacgaatcgaaaa 603 *F Query: 61 catttagttaactcatacaaggtaactgtactaaattatgtacaatatttaaccaacgcc 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 604 catttagttaactcatacaaggtaactgtactaaattatgtacaatatttaaccaacgcc 663 *F Query: 121 tacgactatgtatttagagaattgatcgagttttatgaatgtgataagcggttacatttt 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 664 tacgactatgtatttagagaattgatcgagttttatgaatgtgataagcggttacatttt 723 *F Query: 181 attacacgaacgcgagaaaaagcgaatcccaactaccctttatctccaatactttcgcct 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 724 attacacgaacgcgagaaaaagcgaatcccaactaccctttatctccaatactttcgcct 783 *F Query: 241 ttgccaacactgtcttaa 258 *F |||||||||||||||||| *F Sbjct: 784 ttgccaacactgtcttaa 801 *F >gi|10728458|gb|AE003430.2|AE003430 Drosophila melanogaster genomic scaffold *F 142000013386054 section 14 of *F 35, complete sequence *F Length = 294218 *F Score = 511 bits (258), Expect = e-143 *F Identities = 258/258 (100%) *F Strand = Plus / Plus *F Query: 1 atgtgccctaaagatcgaaaacggagaagcgtttacactgtgctattaacgaatcgaaaa 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268570 atgtgccctaaagatcgaaaacggagaagcgtttacactgtgctattaacgaatcgaaaa *F 268629 *F Query: 61 catttagttaactcatacaaggtaactgtactaaattatgtacaatatttaaccaacgcc 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268630 catttagttaactcatacaaggtaactgtactaaattatgtacaatatttaaccaacgcc *F 268689 *F Query: 121 tacgactatgtatttagagaattgatcgagttttatgaatgtgataagcggttacatttt 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268690 tacgactatgtatttagagaattgatcgagttttatgaatgtgataagcggttacatttt *F 268749 *F Query: 181 attacacgaacgcgagaaaaagcgaatcccaactaccctttatctccaatactttcgcct 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268750 attacacgaacgcgagaaaaagcgaatcccaactaccctttatctccaatactttcgcct *F 268809 *F Query: 241 ttgccaacactgtcttaa 258 *F |||||||||||||||||| *F Sbjct: 268810 ttgccaacactgtcttaa 268827 *F >gi|16924092|gb|AC026773.6| Homo sapiens chromosome 5 clone CTC-230L18, *F complete sequence *F Length = 130965 *F Score = 42.1 bits (21), Expect = 0.24 *F Identities = 21/21 (100%) *F Strand = Plus / Plus *F Query: 127 tatgtatttagagaattgatc 147 *F ||||||||||||||||||||| *F Sbjct: 24493 tatgtatttagagaattgatc 24513 *F >gi|16924090|gb|AC026409.4| Homo sapiens chromosome 5 clone CTC-437M9, *F complete sequence *F Length = 163384 *F Score = 42.1 bits (21), Expect = 0.24 *F Identities = 21/21 (100%) *F Strand = Plus / Minus *F Query: 127 tatgtatttagagaattgatc 147 *F ||||||||||||||||||||| *F Sbjct: 43872 tatgtatttagagaattgatc 43852 *F >gi|4938307|emb|AL049631.7|HSDJ513M9 Human DNA sequence from clone 513M9 on *F chromosome Xq22.1-22.3. Contains *F part of a gene for a novel Homeobox domain protein, ESTs, *F an STS, GSSs and a putative CpG island, complete sequence *F Homo sapiens *F Length = 118047 *F Score = 40.1 bits (20), Expect = 0.95 *F Identities = 26/28 (92%) *F Strand = Plus / Minus *F Query: 85 actgtactaaattatgtacaatatttaa 112 *F ||||||||||||||| || ||||||||| *F Sbjct: 62653 actgtactaaattatttaaaatatttaa 62626 *F >gi|2231346|gb|U78332.1|GPU78332 Galerella pulverulenta 12S ribosomal RNA *F gene, partial sequence, *F tRNA Val gene, complete sequence and 16S ribosomal RNA *F gene, partial sequence, mitochondrial genes encoding *F mitochondrial RNAs *F Length = 1066 *F Score = 40.1 bits (20), Expect = 0.95 *F Identities = 20/20 (100%) *F Strand = Plus / Plus *F Query: 58 aaacatttagttaactcata 77 *F |||||||||||||||||||| *F Sbjct: 617 aaacatttagttaactcata 636 *F >gi|15217430|ref|NC_003070.1| Arabidopsis thaliana chromosome 1, complete *F sequence *F Length = 29640317 *F Score = 38.2 bits (19), Expect = 3.7 *F Identities = 19/19 (100%) *F Strand = Plus / Minus *F Query: 92 taaattatgtacaatattt 110 *F ||||||||||||||||||| *F Sbjct: 13141281 taaattatgtacaatattt 13141263 *F >gi|8778333|gb|AC007887.9|AC007887 Genomic sequence for Arabidopsis thaliana *F BAC F15O4 from chromosome I, *F complete sequence *F Length = 158096 *F Score = 38.2 bits (19), Expect = 3.7 *F Identities = 19/19 (100%) *F Strand = Plus / Plus *F Query: 92 taaattatgtacaatattt 110 *F ||||||||||||||||||| *F Sbjct: 78316 taaattatgtacaatattt 78334 *F >gi|3169212|gb|AC004773.1|AC004773 Homo sapiens PAC clone RP5-982I22 from *F 7q11, complete sequence *F Length = 126995 *F Score = 38.2 bits (19), Expect = 3.7 *F Identities = 19/19 (100%) *F Strand = Plus / Plus *F Query: 56 gaaaacatttagttaactc 74 *F ||||||||||||||||||| *F Sbjct: 21874 gaaaacatttagttaactc 21892 *F Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS, *F or phase 0, 1 or 2 HTGS sequences). *F Posted date: Jan 2, 2002 5:16 AM *F Number of letters in database: 60,694,059 *F Number of sequences in database: 1,057,903 *F Lambda K H *F 1.37 0.711 1.31 *F Gapped *F Lambda K H *F 1.37 0.711 1.31 *F Matrix: blastn matrix:1 \-3 *F Gap Penalties: Existence: 5, Extension: 2 *F Number of Hits to DB: 602,380 *F Number of Sequences: 1079316 *F Number of extensions: 602380 *F Number of successful extensions: 10848 *F Number of sequences better than 10.0: 10 *F length of query: 258 *F length of database: 4,832,507,720 *F effective HSP length: 20 *F effective length of query: 238 *F effective length of database: 4,810,921,400 *F effective search space: 1144999293200 *F effective search space used: 1144999293200 *F T: 0 *F A: 30 *F X1: 6 (11.9 bits) *F X2: 15 (29.7 bits) *F S1: 12 (24.3 bits) *F S2: 19 (38.2 bits) *F \------------------------------------------------------------------------------ *F -- # *U FBrf0145594 *a Shiraiwa *b T. *t 2002.1.27 *T personal communication to FlyBase *u FlyBase error report for geko (gk)(CG13695) on Sun Jan 27 16:02:11 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 27 Jan 2002 16:02:11 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: takashi.shiraiwa@yale.edu *F Subject: FlyBase error report for geko (gk)(CG13695) on Sun Jan 27 16:02:11 2002 *F Error report from Takashi Shiraiwa (takashi.shiraiwa@yale.edu) *F Gene or accession: geko (gk)(CG13695) *F Release: 2 *F cDNA or EST error *F Gene cDNA sequence: *F > *F GAGTGGCATCGCAACTTTTGGACGAATCGGACGTACCGCTCCATATCGTCGTTAT *F ATCTTAGTACCTGTTGCTTTCGCGTGTGTTACCTGTATCTGTATTTTGGTTTTACA *F GTTACTAACGAGTTATGTGCGCTTGAATGCCAAATTTAATTGCCGCTCTTTGCATG *F TTCGTCAGCAACAATTTGAAAACGTTTTCGTAAAGCGATTAAGAAACTACTTTCG *F TTTTTCCCCCCAACTTTCCATTTTCTTGCTCGTCGGCTTGCCATTGAAAAAAGTGT *F CAAAACCCAGGTGCTAACAAACCAAAGCAGAGATCAAACATCTGAAAGAAAAAA *F ATACCGAAAACTATAAAGTTGTGAAACGCTTGTGAATTGATACAGTTTTATATTA *F TGGCTCTCATTGACACCGCCTGGCGTTGTCGCCTCATGATCTTCGTTATCGTCTCG *F CTGCTCCTTGTGGGCGATCTTTTTGGATACGGCGGCGGCATGAAGTCCAACCACCC *F CAATCCGCACTCGGTAAGATCGGCGCGGGGCGCGGTGCCGGGTGGTGCCCCAACGG *F GTCCGGCGGCTGCAGCGGCCAGCAAGTTCCAGACCAAAAATGCCGAAATCGACAT *F AACTGAAGAGCACGATTTCAATGAACTCTCAGCAGCAGCTCAATCCCTCAAGCCT *F GGAATGGCAGTGGTTACTGGGGCTAGTCTGAAGACCAAGTCCCAACTCACGGACG *F CCATCAAGGAATCATGTCTCCCCAAGATGCTCTGCGAACTGGCCTCCAAGGCGGA *F TTACCAACTCAGCGAAAAGGAGCGGGAACTGCTAAGACTGATCAGATCTCCAACA *F ATGCCCTGGATGATGAACATGCCGCCGAGCAAATGGTATTTTGCGGCCCACATGG *F GCGAACTCATGCGTCACACTGGCGACAATCTCAGCGGACCTATGGGATGCGCCAA *F TCTCTGGCCAAATTGCCCGATCAGCTCCAAGAAGCTGATGAAGCTCAGCTACAAA *F GTGAGGGTCTAGCCACTGGATCCGAACCTTAAGTATTAGGATGTAGGCGACCCCC *F CCTCAACTTTTGTACCATATGTCTTCTCACCGTTCGATAAGCTTATCTTGTAAATT *F ACCTTGTAAATTAGATCTAAGTACCGACTTGACTGAAGTGACTGTAGAACTGTAA *F CCTCTTGTATCTGTATTTACTGGCTACAAGATCTAGGTAGCTTGTAATTACATAT *F AGTACGTGTGACTAGTCACGATCCGATTTAGCGTTACGCCTAGTCGTTAGTTGGAT *F GCTCCTGTAATTGTTAATAAATTACACTCTGGTGTGCAAACT *F Protein sequence: *F > *F MALIDTAWRCRLMIFVIVSLLLVGDLFGYGGGMKSNHPNPHSVRSARGAVPGGAPTGP *F AAAAASKFQTKNAEIDITEEHDFNELSAAAQSLKPGMAVVTGASLKTKSQLTDAIKES *F CLPKMLCELASKADYQLSEKERELLRLIRSPTMPWMMNMPPSKWYFAAHMGELMRHT *F GDNLSGPMGCANLWPNCPISSKKLMKLSYKVRV *F Comments: The sequence I submitted here is a sequence from multiple clones *F obtained from a cDNA library. A 10kb genomic sequence around this region was *F used as a probe to screen the cDNA library and no other types of transcripts *F were found. The accession \# is AB008048. # *U FBrf0145596 *a Ayyub *b C. *t 2001.12.14 *T personal communication to FlyBase *u *F Date: Fri, 14 Dec 2001 11:58:22 \-0500 *F To: Gillian Millburn (Genetics) *F From: Kevin Cook *F Subject: P{GawB}neurGAL4-A101 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Champakali Ayyub, Tata Institute of Fundamental Research *F (11/01). *F P{GAL4}neurGAL4-A101 (FBti0017049) was constructed by replacing the *F P{lArB} in P{lArB}neurA101 (FBti0002306) with the P{GawB} from *F P{GawB}SG18.1 via P transposase-mediated conversion. Consequently, *F P{GAL4}neurGAL4-A101 should be denoted P{GawB}neurGAL4-A101. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145597 *a Rauskolb *b C. *t 2002.2.5 *T personal communication to FlyBase *u *F Date: Tue, 05 Feb 2002 17:00:15 \-0500 *F From: cordelia rauskolb *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query *F Hi! *F 1) UASDll \- the one I used does not come from any of the sources you *F mentioned. It's original name was y w hs-FLP122;UAS>CD2 y+>Dll, but it *F had the CD2 y+ cassette FLPed out. I think I got it from Richard Mann *F (Columbia), but I have not seen him ever publish this line. *F 2) UASdac21M5: the reference is Shen and Mardon, 1997 (same reference as *F for UASdac7c4). It is a UASdac on the 3rd while UASdac7c4 is on the 2nd *F chromosome. .. *F Hope this helps. *F Cordelia *F 'Gillian Millburn (Genetics)' wrote: *F > Dear Dr. Rauskolb, *F > *F > I am curating your paper for FlyBase: *F > *F > Rauskolb, 2001, Development 128(22): 4511--4521 *F > *F > and I have a couple of questions. *F > *F > 1. UASDll *F > *F > FlyBase has a record of 3 UAS-Dll constructs: *F > *F > a. 'construct a of Basler' *F > *F > described in: *F > *F > Dong et al., 2000, Development 127(2): 209--216 *F > *F > b. 'construct a of Gorfinkiel' *F > *F > described in: *F > *F > Gorfinkiel et al., 1997, Genes Dev. 11(17): 2259--2271 *F > *F > c. 'construct a of Wu' *F > *F > described in: *F > *F > Wu and Cohen, 1999, Development 126(1): 109--117 *F > *F > do you know which is the one you used ? (if not, who did you get the *F > construct from and do you know a reference where it has been described) *F > *F > 2. UASdac21M5 *F > *F > Is this UAS-dac construct the same as the 'UASdac7c4' construct you *F > also mention in the Materials and Methods or are they different *F > constructs ? *F > *F > Could you tell me who you got the 'UASdac21M5' construct from and a *F > reference where it has been used, so that I can work out which of the *F > UAS-dac constructs in FlyBase it corresponds to, *F > *F > I look forward to hearing from you, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- # *U FBrf0145612 *a Cronmiller *b C. *t 2002.2.26 *T personal communication to FlyBase *u *F From crc2s@unix.mail.virginia.edu Tue Feb 26 21:08:43 2002 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 26 Feb 2002 21:08:43 \+0000 *F Mime-Version: 1.0 *F Date: Tue, 26 Feb 2002 15:58:22 \-0500 *F To: Gillian Millburn (Genetics) *F From: Claire Cronmiller *F Subject: Re: FlyBase query *F Hi Gillian, *F Re: Smith and Cronmiller, 2001, Development 128(23): 4705--4714 *F .. *F Another note on the information in this paper: *F We submitted sequences for each end of the springer element in *F GenBank accession files: AF418012, AF418013. Our deduced full springer *F sequence essentially matches that in GenBank file: AF364549, although the *F predicted ORF2 (pol) differs at the N terminus: *F 'FFRGKSLLPVIRRRVAGIDMKLLIDTGAAKNFIRPFKGLKGVRPVQSPFTIHSIHGVTTITKKCFVSIFNLKATF *F FLLPDLTSFDAIVGLDLLKQAGASLCLXSGKLKWGSGAEQIDFHTCPDVNFTKVDCSDAPPLIKXAFLKMLGNRKK *F AFADPNEALPYNTSVVATIRXVDEEPIYAKLYPYPMGAADFVNGEIQELLKNGIIQKSKSPYNNPIWVVDKKGTDD *F AGNKKMRLVLDFRKLNERTVPDRYPMPNISMILGNLGKAKYFTTLDLKSGYHQITLAERXREKTXFAVNGGKYEFR *F RLPFGLRNAASIFQRTIDDILREQIGKFCYVYVDDVIIFSEDENXHVKHVDWVLKSLYDANMRISAEKSRFFKKSV *F SFLGFIVTNNGAATDPEKVKAIKEFPEPKNVFEVRSFLGLASYYRCFIKDFASIARPISDILKGENGSVSRHRSRS *F IQVEFSEAQQRAFEKLRNILASEDVILRYPDYKKAFDLTTDASAYGIGAVLSQEGRPITMISRTLSDREVNYATNE *F RELLAIVWALAKLRHYLYAVKEINIFTDHQPLTFAVSESNPNAKIKRWKARIDESGARIFYKPGKNNLVADALSRQ *F QLNVVEEQEPESCAATIHSELSLTHTIESTDKPVNCFQNQIILEEARSHWKRTFILFGNKRRHSINFSCKQALLEE *F LANIIIPNGVNAFHCDLHTVALIQDDVVRQFPATKFWHCKNRVTDIFAMQERKEILTVEHNRAHRSAQENVKQVLS *F EYYFPKMTKLASEIAANCKTCAKAKYDRHPKKQELGETPVPTHVGEILHIDIFSTDKKYFLTCVDKFSKFAMVQPI *F LSRTIEDLKAPLLQLMNVFPKAKTIYCDNEPSLKSQTIVAMLENHFGVSISNAPPLHSVSNGQVERFHSTLIELAR *F CLKIDKGISDTVELVLLATARYNMSIHSVINKKPAEVMRADPDDPHTDVQEKIKNAQILTRKRENASRQNRVFQVG *F DKVLVKSNRRLGNKLTPLCEEKIIEADLGTTVLIKGRVVHKDNLK' *F Notes for the flybase daughterless entry, but unrelated to this paper: *F I. I've identified the transposon of the original da1 mutant allele. It *F is 'insertion element 1360.' *F .. *F Thanks, *F Claire # *U FBrf0145613 *a Calvi *b B. *t 2002.2.25 *T personal communication to FlyBase *u *F To: calvi@mail.med.upenn.edu *F Subject: query about Mcm6 paper *F From: Gillian Millburn (Genetics) *F Date: Fri, 22 Feb 2002 12:22:08 \+0000 *F Hi Brian, *F I have now finished curating your MBC paper; *F Schwed et al., 2002, Molec. Biol. Cell 13(2): 607--620 *F and I have a a question about the deletions in 6C made by P-element *F excision. You used 2 EP elements to make deletions: EP(X)1364 and *F EP(X)1445. From looking at Figure 2. I think that: *F a. Df(1)6C-190, Df(1)6C-25 and Df(1)6C-40 were made by excisions from *F EP(X)1445 *F b. Df(1)6C-51 was made by an excision from EP(X)1364 *F could you confirm if this is correct. *F Also, what EP element were each of the following derived from: *F a. Df(1)6C-310 *F b. 6C-166 *F c. 6C-157 *F I will curate your reply as a personal communication to FlyBase, so *F people can see how we know this information, *F thanks, *F Gillian *F Date: Mon, 25 Feb 2002 12:36:25 \-0500 *F To: Gillian Millburn (Genetics) *F From: Brian Calvi *F Subject: Re: query about Mcm6 paper *F >Hi Brian, *F > *F >I have now finished curating your MBC paper; *F > *F >Schwed et al., 2002, Molec. Biol. Cell 13(2): 607--620 *F > *F >and I have a a question about the deletions in 6C made by P-element *F >excision. You used 2 EP elements to make deletions: EP(X)1364 and *F >EP(X)1445. From looking at Figure 2. I think that: *F > *F >a. Df(1)6C-190, Df(1)6C-25 and Df(1)6C-40 were made by excisions from *F >EP(X)1445 *F > *F >b. Df(1)6C-51 was made by an excision from EP(X)1364 *F > *F >could you confirm if this is correct. *F \------------Yes that is correct. *F > *F >Also, what EP element were each of the following derived from: *F > *F >a. Df(1)6C-310 : EP(X)1364 *F > *F For the next two, the information on the starting P is noted below. *F However, since these events complemented MCM6, and since we did not find a *F deletion by Southern, all we really know is that these are X linked *F lethals. .. *F b. 6C-166- EP(X)1445 *F >c. 6C-157- EP(X)1364 *F > *F > *F >I will curate your reply as a personal communication to FlyBase, so *F >people can see how we know this information, *F > *F >thanks, *F > *F >Gillian *F Brian R. Calvi, Ph.D. *F Assistant Professor *F Department of Genetics *F University of Pennsylvania *F School of Medicine *F 415 Curie Blvd., CRB rm 452A *F Philadelphia, PA 19104-6145 *F 215-573-1994 *F 215-573-5892 (FAX) *F Calvi@mail.med.upenn.edu # *U FBrf0145620 *a Berman *b B. *t 2002.3.5 *T personal communication to FlyBase *u *F Date: Tue, 05 Mar 2002 14:42:11 \-0800 *F From: Benjamin Berman *F To: flybase-help@morgan.harvard.edu *F Subject: Hox11-310 / bsh merge? *F Hi there, *F What is the procedure for proposing a merge of two flybase genes? *F FBgn0010392 (Hox11-310) is an 81bp homeobox fragment which was PCRd out *F of the fly genome by homology to a mouse homeodomain. This complete *F segment matches 100% at the DNA level to part of the bsh gene *F (FBgn0010392). It doesn't match anywhere else in the fly genome. *F Should these two FBgns be merged? *F Thanks, *F ben. *F \-- *F Benjamin Berman *F Rubin Lab, 539 Life Sciences Addition *F Department of Molecular and Cell Biology *F University of California, Berkeley *F benb@fruitfly.org # *U FBrf0145621 *a Davis *b T. *t 2002.2.28 *T personal communication to FlyBase *u FlyBase error report for CG15283 on Thu Feb 28 03:21:56 2002. *F Date: Thu, 28 Feb 2002 03:21:56 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@cardiff.ac.uk *F Subject: FlyBase error report for CG15283 on Thu Feb 28 03:21:56 2002 *F Error report from Terence Davis (davist2@cardiff.ac.uk) *F Gene or accession: CG15283 *F Release: 2 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG15283. *F Comments: I have noted the following conservation between gene CG15283 protein *F sequence and: *F 1) CG7224, 50/71 aa = 70% identical at 3' end (CG7224 has a cDNA: GH18422) *F 2) Mus musculus NP_080799.1, 37/66 aa = 56% at 3' end (cDNA) *F 3) homo sapiens XP_059752.1, 35/64 aa = 54% at 3' end (hypothetical) *F 4) Arabidopsis thaliana NP_569049.1, 24/30 aa = 80% at 3' end (expressed) *F This suggests that CG15283 is a genuine gene and that the 3' end of the *F protein is a novel protein domain. Elements of this domain seem to appear also *F in Rickettsia, and several bacterial genomes (not listed here). # *U FBrf0145622 *a Garza *b D. *t 2002.2.9 *T personal communication to FlyBase *u FlyBase error report for CG3327 on Sat Feb 9 08:38:04 2002. *F Date: Sat, 9 Feb 2002 08:38:04 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: dan.garza@pharma.novartis.com *F Subject: FlyBase error report for CG3327 on Sat Feb 9 08:38:04 2002 *F Error report from dan garza (dan.garza@pharma.novartis.com) *F Gene or accession: CG3327 *F Release: 2 *F Gene annotation error *F Genes CG3327 and CG15410 should be merged. *F Comments: 3327(E23) and 15410 ('antifreeze') are actually one gene and should *F be merged, *F as seen in the deposited cDNA sequence from the Hock, et. al PNAS paper.-D.G. # *U FBrf0145623 *a Korey *b C. *t 2002.2.25 *T personal communication to FlyBase *u FlyBase error report for CG1664 on Mon Feb 25 11:40:22 2002. *F Date: Mon, 25 Feb 2002 11:40:22 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: ckorey@post.harvard.edu *F Subject: FlyBase error report for CG1664 on Mon Feb 25 11:40:22 2002 *F Error report from Christopher Korey (ckorey@post.harvard.edu) *F Gene or accession: CG1664 *F Release: 2 *F Gene annotation error *F Gene CG1664 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Exon1:ATCAAGCGAATATACTTCGCTCGCTACATATTTCTAGTTAGTTTTCGTAACTTTTTCGCTTTGGTGGCAATT *F AAATCAAATTTCCTCGAAGAATAACTTGTAAAAAGGTCGCAAACATCCGTCCAGTTTCTGCCAACCATCGGCCACTGGC *F TATCGAAAGAAGATCGCTAGTGAATTAAATTTCACAAAATTGCGTTGTACTTTGGCCGTGGAAATTTGCCAAAAAACAG *F CAGCTACTTTGCTGCTATATTAGCAAATAGGTTCAGGAAGTCGAGAAAAATTTTTCCAGAAGTTGGCAGCAGTTTGTGT *F GAATGCAAAAAATACATCGCACATAGTTTTTTGCCCTCAAGACAGTTTTCTCAGTAATAACAACAGCACCCACACAAAC *F ACACAACACCACTCAGCTAAAAACGTGCTGAAATTCGCATATCTTTTTGTTGCATACGTTTTTGAGTGAATATTAGTAA *F GA *F TGCCCAAACGCGGCGGTGGCAGTAGCCAGCGGTACAACAACAACGTTGGAAATGGCGGCGGACGTTACAACGCTCCCGA *F GGATTTCGATGATTTTG *F >Exon2:ATGTGGAGGATCGCCAGCGACGCAAGGATCGAAACAAGCGGCGCGTCAGCTTTAAGCCCTCCCAATGTCTAC *F ATAACAAAAAGGACATCAAGCTGCGACCCGAAGATTTGCGTCGATGGGACGAGGATGATGACATGAGCGACATGACCAC *F GGCCGTTAAG *F >Exon3:GATAGACCCACCTCCCGACGTCGGGGATCGCCCATTCCGCGCGGCAAGTTCGGCAAACTGATGCCCAACAGC *F TTTGGCTGGTACCAAGTCACG *F >Exon4:TTACAAAACGCCCAGATATACGAAAAGGAAACACTCTTGAGTGCTCTATT *F GGCAGCGATGTCGCCACATGTCTTTATTCCTCAATATTGGCGAGTGGAGCGAAACTGCGTAATCTTCTTTACGGACGAC *F TACGAGGCAGCCGAACGCATTCAACATCTGGGCAAGAATGGCCATCTTCCAGATGGCTATCGTCTGATGCCACGAGTAC *F GCAGCGGTATACCACTAGTGGCCATCGACGATGCCTTCAAGGAGAAGATGAAGGTCACAATGGCCAAGCGTTACAATAT *F TCAAACCAAGGCGCTGGATCTTTCCCGTTTTCATGCAGATCCGGATCTTAAGCAAGTTTTCTGCCCACTCTTTCGTCAG *F AATGTGATGGGCGCTGCCATTGACATTATGTGCGACAATATACCCGATTTGGAGGCACTTAACCTGAATGACAACTCCA *F TTAGCAGCATGGAGGCGTTTAAGGGTGTGGAGAAACGCTTACCGAACCTCAAGATTCTCTATTTGGGGGATAACAAG *F >Exon5:ATACCATCTTTGGCCCACCTTGTAGTGCTTCGCAATCTGTCCATCTTGGAACTCGTTTTAAAGAACAATCCC *F TGCCGTTCCCGCTACAAGGATTCCCAGCAGTTTATCAG *F >Exon6:CGAAGTACGTCGCAAGTTTCCCAAACTGGTTAAGTTG *F >Exon7:GACGGAGAGACCCTGGAGCCGCAAATCACATTTGATCTATCCGAGCAGGGACGTCTTCTCGAAACGAAGGCA *F TCCTATCTGTGCGACGTCGCTGGTGCCGAGGTGGTGCGCCAGTTCCTGGACCAGTACTTCCGCATATTTGACTCGGGCA *F ATCGGCAAGCTCTGCTAGATGCCTACCATGAGAAAGCGATGCTCTCCATATCAATGCCTTCGGCCAGTCAGGCGGGCAG *F >Exon8:ATTGAACAGTTTCTGGAAGTTCAATCGCAATCTCCGGCGCTTGTTAAACGGCGAAGAGAATCGCACCCGAAA *F CTTGAAGTACGGACGCCTGGCATGTGTTTCCACATTGGATGAATGGCCAAAAACGCAGCACGACCGACGCACCTTCACC *F GTCGACCTGACCATCTACAAT *F >Exon9:ACTTCAATGATGGTTTTCACCGTGACGGGATTATTCAAAGAGCTGAACGACGAGACCAACAATCCCGCCTCC *F ATGGAATTATATGACGTTCGCCACTTTGCCCGCACCTACGTGGTGGTGCCACAGAATAATGGCTTTTGTATCCGCAACG *F AGACGATCTTCATCACAAACGCTACGCACGAGCAGGTGCGAGAGTTCAAGCGATCGCAGCACCAGCCTGCTCCCGGAGC *F TATGCCCTCCACTTCCAGTGCAGTGACCAGTCCTCAGGCCGGGGCAGCGGCGGGTCTGCAGGGTCGTCTGAATGCGTTG *F GGCGTGGCCACTGGACCGGTGGCTATACTATCAGGAGATCCGTTGGCGGCCACCGCACCGGTTAACAGCGGCAGTGCCG *F CCATATCGACAACAGCAGTGGCACCTGGCGCCCAGGATGAGAGCACTAAAATGCAAATGATTGAAGCCATGAGCGCCCA *F AAGCCAAATGAATGTGATCTGGAGTCGGAA *F >Exon10:ATGCCTGGAGGAAACGAATTGGGACTTTAACCATGCCGCCTTTGTGTTCGAGAAACTATTCAAGGAAAACA *F AAATACCGCCTGAGGCTTTTATGAAGTAAATCGCATAGGAGTTTCCGTAGGACAGAGCCGCGTGCCACATCCACATAAT *F CGAATGCTGTTTTTTTTTTTTTGGTTTTGTAATTAAATTTTAAAATTATTAGAGAAACCTCTATATAATAATAATAATT *F AATATTATTAAGCTGCGAAGTTGTGTGCACATTCGGGCAGTAGCAATTATTATCCCAGCACTGCGGGCAATGTGCATCA *F ACGATCACAGTTCTTCGATAGATTAGTTTAGCTCTCTTTAAGTTCCGTCCGCAGATCCGCTGGTCTACATTGAGGCCAG *F GACGAGTCTGCGAACGGTAGTCCCTTTAGAGTTAAAGTTGTTTTAGATTCCTAAGCCAAACACCTTCAAACACACACAA *F CACGACAACACTATTAAACGTAATGCAACAATGTTGTCGAATCGAAAGAAACCCAATTTTATTTTAATATATACGACGA *F TACCGAAACCAAGGCGATGGTCGAAAAGCATGGATCGCGCCAATAATTCTATATTCCCCGCTTTCCCAGATCCTCGACT *F CGCCTTACATTTTGTATACAAATACCATAGAATAAAAAGAAACATTTTGACCACTGTAAAAATTTTGTAACGACTCGGA *F AACCAAAATTACCTTTATTTCTTAATAGAAAAAAATTATTCGATTTACAATACACGCTTAGCCGTAAATTCAATTGAAT *F TTAAGTGTAAGATATATAAAAATTATATACATTAAGACAAAAGTGTAGATTCATAAATAAATTTTTCAGAATAG *F Comments: I have just published a paper on sbr. Upon sequencing a full-length *F cDNA clone of the gene we found that it has 10 exons and not the reported 7 in *F the Gadfly annotation of the gene. I pasted in the exon sequences above. *F Chris Korey # *U FBrf0145624 *a Lowe *b T. *t 2002.3.8 *T personal communication to FlyBase *u *F tRNAscan-SE Analysis of the Drosophila melanogaster Genome *F ========================================================== *F [FlyBase curator comment: data taken from the following web pages on *F 8th March 2002: *F http://rna.wustl.edu/GtRDB/Dm/Dm-by-locus.html *F http://rna.wustl.edu/GtRDB/Dm/Dm-seqs.html] *F \------------------------------------------------------------------------------ *F -- *F http://rna.wustl.edu/GtRDB/Dm/Dm-by-locus.html *F tRNAs Sorted by Genome Locus (text) *F Sequence tRNA Bounds tRNA Anti Intron Bounds *F Cove HMM 2'Str *F Name tRNA \# Begin End Type Codon Begin End *F Score Score Score *F \-------- \------ \---- \------ \---- \----- \----- *F \---- \------ \----- \----- *F gb|AE002566|AE002566 1 3630061 3630132 Gln CTG 0 0 *F 72.37 47.72 24.65 Bo *F gb|AE002566|AE002566 2 3637984 3638055 Pro CGG 0 0 *F 72.94 54.02 18.92 Bo *F gb|AE002566|AE002566 3 5641089 5641159 Pseudo CTC 0 0 *F 30.14 27.05 3.09 Eu *F gb|AE002566|AE002566 4 6325411 6325483 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002566|AE002566 5 6552588 6552516 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002566|AE002566 6 3181126 3181055 Gln TTG 0 0 *F 69.58 45.51 24.07 Bo *F gb|AE002575|AE002575 1 380226 380297 Trp CCA 0 0 *F 75.61 53.63 21.98 Bo *F gb|AE002575|AE002575 2 399984 400055 Trp CCA 0 0 *F 75.61 53.63 21.98 Bo *F gb|AE002575|AE002575 3 401556 401626 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002575|AE002575 4 2292805 2292886 Ser TGA 0 0 *F 76.82 48.58 28.24 Bo *F gb|AE002575|AE002575 5 3594348 3594421 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002575|AE002575 6 4289242 4289313 Ala CGC 0 0 *F 76.51 55.23 21.28 Bo *F gb|AE002575|AE002575 7 4290002 4290073 Ala CGC 0 0 *F 76.51 55.23 21.28 Bo *F gb|AE002575|AE002575 8 4290285 4290356 Ala CGC 0 0 *F 76.51 55.23 21.28 Bo *F gb|AE002575|AE002575 9 2292324 2292243 Ser TGA 0 0 *F 79.78 44.11 35.67 Bo *F gb|AE002575|AE002575 10 1067996 1067925 Gly TCC 0 0 *F 72.21 53.21 19.00 Bo *F gb|AE002575|AE002575 11 1062686 1062615 Gly TCC 0 0 *F 72.21 53.21 19.00 Bo *F gb|AE002575|AE002575 12 401449 401378 Trp CCA 0 0 *F 75.61 53.63 21.98 Bo *F gb|AE002575|AE002575 13 380126 380056 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002575|AE002575 14 379834 379763 Trp CCA 0 0 *F 75.61 53.63 21.98 Bo *F gb|AE002584|AE002584 1 750630 750727 Ile TAT 750668 750691 *F 65.59 37.40 28.19 Bo *F gb|AE002584|AE002584 2 751490 751589 Ile TAT 751528 751553 *F 65.99 37.79 28.20 Bo *F gb|AE002584|AE002584 3 1359129 1359200 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 4 1359433 1359504 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 5 1359724 1359795 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 6 1376890 1376961 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 7 1377127 1377198 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 8 1377364 1377435 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 9 1377601 1377672 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 10 1380635 1380706 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 11 1380976 1381047 Glu CTC 0 0 *F 77.51 54.01 23.50 Bo *F gb|AE002584|AE002584 12 1904808 1904879 Cys GCA 0 0 *F 75.79 54.83 20.96 Bo *F gb|AE002584|AE002584 13 2248908 2248979 Cys GCA 0 0 *F 74.46 55.38 19.08 Bo *F gb|AE002584|AE002584 14 3069999 3070070 Cys GCA 0 0 *F 75.91 56.37 19.54 Bo *F gb|AE002584|AE002584 15 3070253 3070324 Cys GCA 0 0 *F 75.91 56.37 19.54 Bo *F gb|AE002584|AE002584 16 3070631 3070702 Cys GCA 0 0 *F 75.91 56.37 19.54 Bo *F gb|AE002584|AE002584 17 3070866 3070938 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002584|AE002584 18 3071781 3071852 Cys GCA 0 0 *F 75.91 56.37 19.54 Bo *F gb|AE002584|AE002584 19 3096276 3096204 Met CAT 0 0 *F 79.03 58.28 20.75 Bo *F gb|AE002584|AE002584 20 3071225 3071153 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002584|AE002584 21 724921 724850 Met CAT 0 0 *F 70.82 45.40 25.42 Bo *F gb|AE002593|AE002593 1 3211241 3211313 Arg TCG 0 0 *F 72.45 52.79 19.66 Bo *F gb|AE002593|AE002593 2 3211472 3211553 Ser CGA 0 0 *F 86.41 56.90 29.51 Bo *F gb|AE002593|AE002593 3 3211872 3211953 Ser CGA 0 0 *F 86.41 56.90 29.51 Bo *F gb|AE002593|AE002593 4 3212630 3212702 Arg TCG 0 0 *F 72.12 53.15 18.97 Bo *F gb|AE002593|AE002593 5 3213235 3213307 Arg TCG 0 0 *F 72.12 53.15 18.97 Bo *F gb|AE002593|AE002593 6 3213437 3213509 Arg TCG 0 0 *F 72.12 53.15 18.97 Bo *F gb|AE002593|AE002593 7 3213639 3213711 Arg TCG 0 0 *F 72.12 53.15 18.97 Bo *F gb|AE002593|AE002593 8 3255817 3255898 Ser AGA 0 0 *F 85.81 56.30 29.51 Bo *F gb|AE002593|AE002593 9 3289895 3289976 Ser AGA 0 0 *F 83.36 53.85 29.51 Bo *F gb|AE002593|AE002593 10 7738243 7738314 Pro CGG 0 0 *F 75.20 53.14 22.06 Bo *F gb|AE002593|AE002593 11 3303326 3303245 Ser AGA 0 0 *F 84.54 52.25 32.29 Bo *F gb|AE002593|AE002593 12 3289037 3288965 Arg TCG 0 0 *F 74.10 54.44 19.66 Bo *F gb|AE002593|AE002593 13 3288689 3288608 Ser CGA 0 0 *F 86.41 56.90 29.51 Bo *F gb|AE002593|AE002593 14 3222074 3221993 Ser AGA 0 0 *F 84.54 52.25 32.29 Bo *F gb|AE002593|AE002593 15 3221617 3221536 Ser AGA 0 0 *F 83.36 53.85 29.51 Bo *F gb|AE002602|AE002602 1 2941402 2941473 Pro CGG 0 0 *F 75.20 53.14 22.06 Bo *F gb|AE002602|AE002602 2 5609856 5609927 Met CAT 0 0 *F 70.82 45.40 25.42 Bo *F gb|AE002602|AE002602 3 6830528 6830600 Val AAC 0 0 *F 79.44 53.84 25.60 Bo *F gb|AE002602|AE002602 4 8462145 8462216 Gln TTG 0 0 *F 72.03 47.96 24.07 Bo *F gb|AE002602|AE002602 5 12189830 12189912 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002602|AE002602 6 12192044 12192126 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002602|AE002602 7 12192395 12192477 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002602|AE002602 8 12197364 12197446 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002602|AE002602 9 12197587 12197669 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002602|AE002602 10 12197824 12197906 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002602|AE002602 11 12229502 12229574 Ala AGC *F 0 0 59.84 35.65 24.19 Bo *F gb|AE002602|AE002602 12 14869231 14869303 Val TAC *F 0 0 79.18 50.73 28.45 Bo *F gb|AE002602|AE002602 13 14883881 14883800 Ser AGA *F 0 0 84.54 52.25 32.29 Bo *F gb|AE002602|AE002602 14 14869622 14869550 Val TAC *F 0 0 79.18 50.73 28.45 Bo *F gb|AE002602|AE002602 15 11356075 11356002 Pseudo AGT *F 0 0 52.51 48.08 4.43 Eu *F gb|AE002602|AE002602 16 7053720 7053649 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002602|AE002602 17 7053383 7053312 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002602|AE002602 18 7052569 7052498 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002602|AE002602 19 7050825 7050701 Leu CAA 7050787 *F 7050746 68.33 43.37 24.96 Bo *F gb|AE002602|AE002602 20 7015387 7015316 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002602|AE002602 21 6985166 6985085 Leu AAG 0 0 *F 71.48 46.08 25.40 Bo *F gb|AE002602|AE002602 22 6831198 6831126 Val AAC 0 0 *F 79.44 53.84 25.60 Bo *F gb|AE002602|AE002602 23 5609525 5609454 Met CAT 0 0 *F 70.82 45.40 25.42 Bo *F gb|AE002602|AE002602 24 4057326 4057254 Met CAT 0 0 *F 79.43 59.05 20.38 Bo *F gb|AE002602|AE002602 25 3929593 3929521 Met CAT 0 0 *F 79.43 59.05 20.38 Bo *F gb|AE002602|AE002602 26 1674504 1674433 Pro CGG 0 0 *F 75.20 53.14 22.06 Bo *F gb|AE002620|AE002620 1 216526 216621 Tyr GTA 216564 216585 *F 67.06 42.42 24.64 Eu *F gb|AE002620|AE002620 2 289667 289741 Pseudo AAT 0 0 *F 44.17 41.28 2.89 Eu *F gb|AE002620|AE002620 3 288975 288903 Arg TCG 0 0 *F 72.45 52.79 19.66 Bo *F gb|AE002620|AE002620 4 216345 216238 Tyr GTA 216308 216274 *F 75.42 50.37 25.05 Bo *F gb|AE002638|AE002638 1 524380 524451 Trp CCA 0 0 *F 75.61 53.63 21.98 Bo *F gb|AE002638|AE002638 2 1736395 1736476 Ser AGA 0 0 *F 84.54 52.25 32.29 Bo *F gb|AE002638|AE002638 3 2482775 2482895 Tyr GTA 2482812 *F 2482859 73.79 48.74 25.05 Bo *F gb|AE002638|AE002638 4 2921074 2921145 Gln CTG 0 0 *F 72.37 47.72 24.65 Bo *F gb|AE002638|AE002638 5 2923384 2923455 Gln CTG 0 0 *F 71.12 45.68 25.44 Bo *F gb|AE002638|AE002638 6 2923607 2923678 Gln CTG 0 0 *F 72.37 47.72 24.65 Bo *F gb|AE002638|AE002638 7 2996165 2996235 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002638|AE002638 8 2968898 2968826 Arg TCT 0 0 *F 74.52 53.61 20.91 Bo *F gb|AE002638|AE002638 9 2925169 2925098 Gln CTG 0 0 *F 72.37 47.72 24.65 Bo *F gb|AE002638|AE002638 10 2924786 2924715 Gln CTG 0 0 *F 72.37 47.72 24.65 Bo *F gb|AE002638|AE002638 11 2902631 2902561 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002638|AE002638 12 2755501 2755429 Arg TCT 0 0 *F 66.34 51.32 15.02 Bo *F gb|AE002638|AE002638 13 2480226 2480133 Tyr GTA 2480189 *F 2480169 77.84 52.80 25.04 Bo *F gb|AE002638|AE002638 14 1739897 1739816 Ser AGA 0 0 *F 84.54 52.25 32.29 Bo *F gb|AE002647|AE002647 1 2494080 2494162 Leu CAG 0 0 *F 77.88 47.49 30.39 Bo *F gb|AE002647|AE002647 2 2498612 2498541 Glu CTC 0 0 *F 77.90 54.75 23.15 Bo *F gb|AE002647|AE002647 3 1512550 1512479 Cys GCA 0 0 *F 70.54 51.46 19.08 Bo *F gb|AE002681|AE002681 1 944620 944548 Arg TCG 0 0 *F 69.30 49.64 19.66 Bo *F gb|AE002690|AE002690 1 1837367 1837439 Arg CCT 0 0 *F 67.50 54.15 13.35 Bo *F gb|AE002690|AE002690 2 2909049 2909119 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002690|AE002690 3 3330035 3330107 Lys TTT 0 0 *F 84.71 64.52 20.19 Bo *F gb|AE002690|AE002690 4 3529278 3529349 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002690|AE002690 5 3618394 3618466 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002690|AE002690 6 3667385 3667456 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002690|AE002690 7 3726475 3726546 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002690|AE002690 8 3730810 3730881 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002690|AE002690 9 9522008 9522079 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002690|AE002690 10 9544221 9544291 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002690|AE002690 11 9624987 9625058 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002690|AE002690 12 9625802 9625873 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002690|AE002690 13 10156452 10156369 Leu TAA *F 0 0 68.86 41.26 27.60 Bo *F gb|AE002690|AE002690 14 9625595 9625524 Pro AGG 0 0 *F 72.31 53.15 19.16 Bo *F gb|AE002690|AE002690 15 9557054 9556984 Gly GCC 0 0 *F 77.95 61.96 15.99 Bo *F gb|AE002690|AE002690 16 9556746 9556676 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002690|AE002690 17 9544821 9544751 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002690|AE002690 18 9544507 9544437 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002690|AE002690 19 9037163 9037092 Gln CTG 0 0 *F 69.04 48.11 20.93 Bo *F gb|AE002690|AE002690 20 4283294 4283221 Thr AGT 0 0 *F 75.61 60.92 14.69 Bo *F gb|AE002690|AE002690 21 3731236 3731165 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002690|AE002690 22 3529657 3529585 Asp GTC 0 0 *F 60.88 42.97 17.91 Bo *F gb|AE002690|AE002690 23 298889 298818 Asp GTC 0 0 *F 75.34 51.53 23.81 Bo *F gb|AE002699|AE002699 1 220703 220775 Val CAC 0 0 *F 81.63 56.10 25.53 Bo *F gb|AE002699|AE002699 2 224546 224618 Val CAC 0 0 *F 81.63 56.10 25.53 Bo *F gb|AE002699|AE002699 3 565737 565808 Gly TCC 0 0 *F 76.96 57.96 19.00 Bo *F gb|AE002699|AE002699 4 874598 874670 Lys TTT 0 0 *F 84.31 63.76 20.55 Bo *F gb|AE002699|AE002699 5 876900 876972 Lys TTT 0 0 *F 76.87 60.23 16.64 Bo *F gb|AE002699|AE002699 6 878702 878774 Pseudo CTT 0 0 *F 28.65 33.51 \-4.86 Eu *F gb|AE002699|AE002699 7 1201937 1202009 Met CAT 0 0 *F 79.43 59.05 20.38 Bo *F gb|AE002699|AE002699 8 878146 878074 Lys TTT 0 0 *F 84.31 63.76 20.55 Bo *F gb|AE002699|AE002699 9 876315 876243 Lys TTT 0 0 *F 84.31 63.76 20.55 Bo *F gb|AE002699|AE002699 10 875844 875772 Lys TTT 0 0 *F 84.31 63.76 20.55 Bo *F gb|AE002699|AE002699 11 566891 566820 Gly TCC 0 0 *F 76.96 57.96 19.00 Bo *F gb|AE002699|AE002699 12 245381 245309 Val CAC 0 0 *F 81.63 56.10 25.53 Bo *F gb|AE002699|AE002699 13 244618 244546 Val CAC 0 0 *F 81.63 56.10 25.53 Bo *F gb|AE002708|AE002708 1 1149394 1149466 Arg TCG 0 0 *F 72.45 52.79 19.66 Bo *F gb|AE002708|AE002708 2 1150447 1150519 Arg TCG 0 0 *F 72.45 52.79 19.66 Bo *F gb|AE002708|AE002708 3 4398969 4399041 Lys TTT 0 0 *F 84.31 63.76 20.55 Bo *F gb|AE002708|AE002708 4 4474188 4474259 Thr TGT 0 0 *F 74.00 47.31 26.69 Bo *F gb|AE002708|AE002708 5 6029215 6029287 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002708|AE002708 6 8554738 8554810 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002708|AE002708 7 8555025 8555097 Val AAC 0 0 *F 80.36 54.92 25.44 Bo *F gb|AE002708|AE002708 8 8665329 8665402 Thr AGT 0 0 *F 75.61 60.92 14.69 Bo *F gb|AE002708|AE002708 9 9851236 9851308 Val CAC 0 0 *F 83.37 55.06 28.31 Bo *F gb|AE002708|AE002708 10 9853213 9853285 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 11 9853436 9853507 Pro TGG 0 0 *F 75.74 54.04 21.70 Bo *F gb|AE002708|AE002708 12 9856021 9856093 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 13 9865154 9865225 Pro TGG 0 0 *F 75.74 54.04 21.70 Bo *F gb|AE002708|AE002708 14 9879085 9879156 Pro TGG 0 0 *F 75.74 54.04 21.70 Bo *F gb|AE002708|AE002708 15 9879843 9879915 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 16 9890622 9890694 Ala AGC 0 0 *F 63.54 39.35 24.19 Bo *F gb|AE002708|AE002708 17 9901664 9901736 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 18 10343027 10343098 Gly TCC *F 0 0 76.96 57.96 19.00 Bo *F gb|AE002708|AE002708 19 10343383 10343454 Gly TCC *F 0 0 76.96 57.96 19.00 Bo *F gb|AE002708|AE002708 20 12017657 12017729 Val CAC *F 0 0 81.63 56.10 25.53 Bo *F gb|AE002708|AE002708 21 12018507 12018579 Ala AGC *F 0 0 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 22 12030225 12030298 Thr AGT *F 0 0 75.61 60.92 14.69 Bo *F gb|AE002708|AE002708 23 13116872 13116943 Thr CGT *F 0 0 77.47 52.68 24.79 Bo *F gb|AE002708|AE002708 24 13117296 13117367 Thr CGT *F 0 0 77.47 52.68 24.79 Bo *F gb|AE002708|AE002708 25 13117832 13117903 Thr CGT *F 0 0 77.47 52.68 24.79 Bo *F gb|AE002708|AE002708 26 14612289 14612370 Ser GCT *F 0 0 82.84 56.17 26.67 Bo *F gb|AE002708|AE002708 27 14613334 14613415 Ser GCT *F 0 0 82.84 56.17 26.67 Bo *F gb|AE002708|AE002708 28 16328484 16328567 Leu TAA *F 0 0 69.13 41.53 27.60 Bo *F gb|AE002708|AE002708 29 16480680 16480763 Leu TAA *F 0 0 68.33 41.59 26.74 Bo *F gb|AE002708|AE002708 30 16770750 16770821 Asp GTC *F 0 0 75.34 51.53 23.81 Bo *F gb|AE002708|AE002708 31 17130279 17130350 Asp GTC *F 0 0 75.34 51.53 23.81 Bo *F gb|AE002708|AE002708 32 22437471 22437392 Leu TAG *F 0 0 69.19 43.41 25.78 Bo *F gb|AE002708|AE002708 33 22436819 22436740 Leu TAG *F 0 0 69.19 43.41 25.78 Bo *F gb|AE002708|AE002708 34 19158558 19158487 Gln TTG *F 0 0 72.03 47.96 24.07 Bo *F gb|AE002708|AE002708 35 19158309 19158238 Gln TTG *F 0 0 72.03 47.96 24.07 Bo *F gb|AE002708|AE002708 36 17130623 17130552 Asp GTC *F 0 0 68.81 50.44 18.37 Bo *F gb|AE002708|AE002708 37 15816611 15816489 Leu CAA *F 15816573 15816534 65.38 40.41 24.97 Bo *F gb|AE002708|AE002708 38 14613023 14612942 Ser GCT *F 0 0 87.38 60.71 26.67 Bo *F gb|AE002708|AE002708 39 14612642 14612561 Ser GCT *F 0 0 82.84 56.17 26.67 Bo *F gb|AE002708|AE002708 40 12830608 12830525 Leu TAA *F 0 0 70.06 40.33 29.73 Bo *F gb|AE002708|AE002708 41 11899837 11899765 Val CAC *F 0 0 81.63 56.10 25.53 Bo *F gb|AE002708|AE002708 42 10862802 10862731 Pro TGG *F 0 0 75.74 54.04 21.70 Bo *F gb|AE002708|AE002708 43 9891930 9891858 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 44 9880232 9880160 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 45 9878854 9878782 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 46 9864589 9864517 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 47 9862633 9862561 Val AAC 0 0 *F 80.36 54.92 25.44 Bo *F gb|AE002708|AE002708 48 9858354 9858281 Thr AGT 0 0 *F 75.61 60.92 14.69 Bo *F gb|AE002708|AE002708 49 9858113 9858040 Thr AGT 0 0 *F 75.61 60.92 14.69 Bo *F gb|AE002708|AE002708 50 9853691 9853619 Ala AGC 0 0 *F 59.84 35.65 24.19 Bo *F gb|AE002708|AE002708 51 9851656 9851585 Pro TGG 0 0 *F 75.74 54.04 21.70 Bo *F gb|AE002708|AE002708 52 8658449 8658376 Thr AGT 0 0 *F 75.61 60.92 14.69 Bo *F gb|AE002708|AE002708 53 6275877 6275796 Ser GCT 0 0 *F 82.84 56.17 26.67 Bo *F gb|AE002708|AE002708 54 6029582 6029510 Phe GAA 0 0 *F 75.08 53.59 21.49 Bo *F gb|AE002708|AE002708 55 4590246 4590175 Thr TGT 0 0 *F 76.08 51.64 24.44 Bo *F gb|AE002708|AE002708 56 2547881 2547800 Ser GCT 0 0 *F 82.84 56.17 26.67 Bo *F gb|AE002708|AE002708 57 956764 956671 Tyr GTA 956727 956707 *F 79.41 54.36 25.05 Bo *F gb|AE002708|AE002708 58 956295 956202 Tyr GTA 956258 956238 *F 79.90 54.85 25.05 Bo *F gb|AE002708|AE002708 59 955798 955706 Tyr GTA 955761 955742 *F 79.14 54.09 25.05 Bo *F gb|AE002708|AE002708 60 930384 930292 Tyr GTA 930347 930328 *F 79.28 54.23 25.05 Bo *F gb|AE002708|AE002708 61 930016 929924 Tyr GTA 929979 929960 *F 78.51 53.46 25.05 Bo *F gb|AE002708|AE002708 62 426621 426549 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002708|AE002708 63 426378 426306 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002708|AE002708 64 426127 426055 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002708|AE002708 65 425874 425802 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002708|AE002708 66 425602 425530 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002708|AE002708 67 425185 425112 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002769|AE002769 1 66475 66548 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002769|AE002769 2 78021 78093 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002769|AE002769 3 85109 85181 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002769|AE002769 4 91729 91801 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002769|AE002769 5 93924 93996 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002769|AE002769 6 94670 94743 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002769|AE002769 7 103187 103260 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002769|AE002769 8 104090 104163 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002769|AE002769 9 114322 114394 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002769|AE002769 10 114601 114529 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002769|AE002769 11 103580 103507 Asn GTT 0 0 *F 86.74 65.43 21.31 Bo *F gb|AE002769|AE002769 12 95231 95159 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002769|AE002769 13 94158 94085 Asn GTT 0 0 *F 83.77 65.95 17.82 Bo *F gb|AE002769|AE002769 14 90693 90621 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002769|AE002769 15 90526 90453 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002769|AE002769 16 87070 86998 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002769|AE002769 17 84990 84918 Arg ACG 0 0 *F 73.12 53.28 19.84 Bo *F gb|AE002769|AE002769 18 66305 66232 Asn GTT 0 0 *F 76.75 59.45 17.30 Bo *F gb|AE002778|AE002778 1 457256 457184 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002778|AE002778 2 456407 456335 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002778|AE002778 3 455861 455789 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002778|AE002778 4 454931 454859 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002787|AE002787 1 122202 122274 Trp CCA 0 0 *F 64.90 48.12 16.78 Eu *F gb|AE002787|AE002787 2 779542 779614 Arg CCT 0 0 *F 67.50 54.15 13.35 Bo *F gb|AE002787|AE002787 3 1027003 1027074 Glu CTC 0 0 *F 77.90 54.75 23.15 Bo *F gb|AE002787|AE002787 4 1027210 1027281 Glu TTC 0 0 *F 80.27 56.60 23.67 Bo *F gb|AE002787|AE002787 5 1027731 1027802 Glu TTC 0 0 *F 80.27 56.60 23.67 Bo *F gb|AE002787|AE002787 6 1029049 1029120 Glu TTC 0 0 *F 80.27 56.60 23.67 Bo *F gb|AE002787|AE002787 7 1029571 1029642 Met CAT 0 0 *F 70.82 45.40 25.42 Bo *F gb|AE002787|AE002787 8 1189234 1189305 Glu TTC 0 0 *F 80.27 56.60 23.67 Bo *F gb|AE002787|AE002787 9 1247768 1247839 His GTG 0 0 *F 67.15 42.32 24.83 Bo *F gb|AE002787|AE002787 10 2114387 2114457 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002787|AE002787 11 3138166 3138238 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002787|AE002787 12 3350803 3350874 Ala TGC 0 0 *F 75.92 53.87 22.05 Bo *F gb|AE002787|AE002787 13 3597984 3598065 Leu AAG 0 0 *F 71.48 46.08 25.40 Bo *F gb|AE002787|AE002787 14 4533329 4533400 Gln CTG 0 0 *F 73.65 50.36 23.29 Bo *F gb|AE002787|AE002787 15 5753326 5753407 Leu AAG 0 0 *F 71.48 46.08 25.40 Bo *F gb|AE002787|AE002787 16 7183504 7183576 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002787|AE002787 17 7288808 7288928 Leu CAA 7288846 *F 7288883 72.14 47.17 24.97 Bo *F gb|AE002787|AE002787 18 7289268 7289341 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 19 8381977 8382048 His GTG 0 0 *F 67.15 42.32 24.83 Bo *F gb|AE002787|AE002787 20 8382738 8382809 His GTG 0 0 *F 67.15 42.32 24.83 Bo *F gb|AE002787|AE002787 21 8383046 8383117 His GTG 0 0 *F 67.15 42.32 24.83 Bo *F gb|AE002787|AE002787 22 9056371 9056443 Met CAT 0 0 *F 79.43 59.05 20.38 Bo *F gb|AE002787|AE002787 23 9064567 9064639 Arg TCT 0 0 *F 82.67 62.26 20.41 Bo *F gb|AE002787|AE002787 24 9311533 9311604 Thr TGT 0 0 *F 76.08 51.64 24.44 Bo *F gb|AE002787|AE002787 25 9316995 9317066 Thr TGT 0 0 *F 76.08 51.64 24.44 Bo *F gb|AE002787|AE002787 26 9359186 9359271 SeC(e) TCA 0 0 *F 56.66 0.00 0.00 Eu *F gb|AE002787|AE002787 27 13603515 13603588 Thr AGT *F 0 0 75.61 60.92 14.69 Bo *F gb|AE002787|AE002787 28 13604305 13604378 Thr AGT *F 0 0 69.29 55.02 14.27 Bo *F gb|AE002787|AE002787 29 12523500 12523427 Ile AAT *F 0 0 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 30 11998761 11998679 Leu CAG *F 0 0 77.88 47.49 30.39 Bo *F gb|AE002787|AE002787 31 11028819 11028748 Met CAT *F 0 0 66.24 40.98 25.26 Bo *F gb|AE002787|AE002787 32 9322483 9322412 Thr TGT 0 0 *F 76.08 51.64 24.44 Bo *F gb|AE002787|AE002787 33 9312137 9312066 Thr TGT 0 0 *F 76.08 51.64 24.44 Bo *F gb|AE002787|AE002787 34 9064357 9064285 Met CAT 0 0 *F 79.43 59.05 20.38 Bo *F gb|AE002787|AE002787 35 8380625 8380554 His GTG 0 0 *F 67.15 42.32 24.83 Bo *F gb|AE002787|AE002787 36 8380310 8380239 Undet ? 0 0 *F 37.58 28.88 8.70 Eu *F gb|AE002787|AE002787 37 7288639 7288566 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 38 7288338 7288265 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 39 7287846 7287773 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 40 7287530 7287457 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 41 7286955 7286829 Leu CAA 7286917 *F 7286874 72.67 43.62 29.05 Bo *F gb|AE002787|AE002787 42 7281754 7281681 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 43 4521343 4521272 Glu TTC 0 0 *F 80.27 56.60 23.67 Bo *F gb|AE002787|AE002787 44 3774022 3773952 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002787|AE002787 45 3596730 3596649 Leu AAG 0 0 *F 71.48 46.08 25.40 Bo *F gb|AE002787|AE002787 46 3350433 3350362 Ala TGC 0 0 *F 74.19 52.13 22.06 Bo *F gb|AE002787|AE002787 47 3138002 3137930 Phe GAA 0 0 *F 82.98 60.05 22.93 Bo *F gb|AE002787|AE002787 48 2114626 2114555 Trp CCA 0 0 *F 75.61 53.63 21.98 Bo *F gb|AE002787|AE002787 49 1984053 1983972 Leu AAG 0 0 *F 71.48 46.08 25.40 Bo *F gb|AE002787|AE002787 50 1339580 1339499 Ser CGA 0 0 *F 86.41 56.90 29.51 Bo *F gb|AE002787|AE002787 51 1339333 1339261 Val AAC 0 0 *F 79.44 53.84 25.60 Bo *F gb|AE002787|AE002787 52 1338907 1338835 Val AAC 0 0 *F 79.44 53.84 25.60 Bo *F gb|AE002787|AE002787 53 1039550 1039477 Ile AAT 0 0 *F 82.79 63.27 19.52 Bo *F gb|AE002787|AE002787 54 1029357 1029286 Met CAT 0 0 *F 70.82 45.40 25.42 Bo *F gb|AE002787|AE002787 55 1026375 1026304 Glu CTC 0 0 *F 77.90 54.75 23.15 Bo *F gb|AE002787|AE002787 56 978050 977978 Lys CTT 0 0 *F 80.47 57.81 22.66 Bo *F gb|AE002787|AE002787 57 779091 779019 Arg CCT 0 0 *F 67.50 54.15 13.35 Bo *F gb|AE002787|AE002787 58 550417 550347 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002787|AE002787 59 547101 547031 Gly GCC 0 0 *F 82.25 63.27 18.98 Bo *F gb|AE002787|AE002787 60 122487 122416 Trp CCA 0 0 *F 75.41 53.26 22.15 Bo *F gi|7289277|gb|AE002611.1|AE002611 1 67042 67113 Pro CGG *F 0 0 76.52 54.46 22.06 Bo *F \------------------------------------------------------------------------------ *F -- *F http://rna.wustl.edu/GtRDB/Dm/Dm-seqs.html *F tRNAs in FASTA sequence format *F >gb|AE002602|AE002602.trna11-AlaAGC (12229502-12229574) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna10-AlaAGC (9853213-9853285) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna12-AlaAGC (9856021-9856093) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna15-AlaAGC (9879843-9879915) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna17-AlaAGC (9901664-9901736) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna21-AlaAGC (12018507-12018579) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna43-AlaAGC (9891930-9891858) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna44-AlaAGC (9880232-9880160) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna45-AlaAGC (9878854-9878782) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna46-AlaAGC (9864589-9864517) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna50-AlaAGC (9853691-9853619) Ala (AGC) 73 bp Sc: *F 59.84 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATG *F CCCCGCATCTCCA *F >gb|AE002708|AE002708.trna16-AlaAGC (9890622-9890694) Ala (AGC) 73 bp Sc: *F 63.54 *F GGGGATGTAGCTCAGATGGTAGAGCGCTCGCTTAGCATGTGAGAGGTACGGGGATCGATA *F CCCCGCATCTCCA *F >gb|AE002575|AE002575.trna6-AlaCGC (4289242-4289313) Ala (CGC) 72 bp Sc: 76.51 *F GGGGACGTAGCTCAGTGGTAGAGCGCTCGCTTCGCATGTGAGAAGTCCCGGGTTCAAACC *F CCGGCGTCTCCA *F >gb|AE002575|AE002575.trna7-AlaCGC (4290002-4290073) Ala (CGC) 72 bp Sc: 76.51 *F GGGGACGTAGCTCAGTGGTAGAGCGCTCGCTTCGCATGTGAGAAGTCCCGGGTTCAAACC *F CCGGCGTCTCCA *F >gb|AE002575|AE002575.trna8-AlaCGC (4290285-4290356) Ala (CGC) 72 bp Sc: 76.51 *F GGGGACGTAGCTCAGTGGTAGAGCGCTCGCTTCGCATGTGAGAAGTCCCGGGTTCAAACC *F CCGGCGTCTCCA *F >gb|AE002787|AE002787.trna46-AlaTGC (3350433-3350362) Ala (TGC) 72 bp Sc: *F 74.19 *F GGGGATGTAGCTCAGTGGTAGAGCGCTCGCTTTGCATGTGAGAGGCCCCGGGTTCAATCC *F CCGGCATCTCCA *F >gb|AE002787|AE002787.trna12-AlaTGC (3350803-3350874) Ala (TGC) 72 bp Sc: *F 75.92 *F GGGGATGTAGCTCAGTGGTAGAGCGCTCGCTTTGCATGTGAGAGGCCCCGGGTTCGATCC *F CCGGCATCTCCA *F >gb|AE002708|AE002708.trna62-ArgACG (426621-426549) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002708|AE002708.trna63-ArgACG (426378-426306) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002708|AE002708.trna64-ArgACG (426127-426055) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002708|AE002708.trna65-ArgACG (425874-425802) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002708|AE002708.trna66-ArgACG (425602-425530) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002769|AE002769.trna10-ArgACG (114601-114529) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002769|AE002769.trna12-ArgACG (95231-95159) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002769|AE002769.trna16-ArgACG (87070-86998) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002769|AE002769.trna17-ArgACG (84990-84918) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002769|AE002769.trna2-ArgACG (78021-78093) Arg (ACG) 73 bp Sc: 73.12 *F GGTCCTGTGGCGCAATGGATAACGCGTCTGACTACGGATCAGAAGATTCCAGGTTCGACT *F CCTGGCAGGATCG *F >gb|AE002690|AE002690.trna1-ArgCCT (1837367-1837439) Arg (CCT) 73 bp Sc: 67.50 *F GCCCCAGTGGCCTAATGGATAAGGCATCGGCCTCCTAAGCCGGGGATTGTGGGTTCGAGT *F CCCATCTGGGGTA *F >gb|AE002787|AE002787.trna2-ArgCCT (779542-779614) Arg (CCT) 73 bp Sc: 67.50 *F GCCCCAGTGGCCTAATGGATAAGGCATCGGCCTCCTAAGCCGGGGATTGTGGGTTCGAGT *F CCCATCTGGGGTA *F >gb|AE002787|AE002787.trna57-ArgCCT (779091-779019) Arg (CCT) 73 bp Sc: 67.50 *F GCCCCAGTGGCCTAATGGATAAGGCATCGGCCTCCTAAGCCGGGGATTGTGGGTTCGAGT *F CCCATCTGGGGTA *F >gb|AE002681|AE002681.trna1-ArgTCG (944620-944548) Arg (TCG) 73 bp Sc: 69.30 *F GACCGTGTGGCCTAAAGGATAAGGCGTCGGACTTCGAATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002593|AE002593.trna4-ArgTCG (3212630-3212702) Arg (TCG) 73 bp Sc: 72.12 *F GACCGTGTGGCCCAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002593|AE002593.trna5-ArgTCG (3213235-3213307) Arg (TCG) 73 bp Sc: 72.12 *F GACCGTGTGGCCCAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002593|AE002593.trna6-ArgTCG (3213437-3213509) Arg (TCG) 73 bp Sc: 72.12 *F GACCGTGTGGCCCAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002593|AE002593.trna7-ArgTCG (3213639-3213711) Arg (TCG) 73 bp Sc: 72.12 *F GACCGTGTGGCCCAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002593|AE002593.trna1-ArgTCG (3211241-3211313) Arg (TCG) 73 bp Sc: 72.45 *F GACCGTGTGGCCTAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002620|AE002620.trna3-ArgTCG (288975-288903) Arg (TCG) 73 bp Sc: 72.45 *F GACCGTGTGGCCTAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002708|AE002708.trna1-ArgTCG (1149394-1149466) Arg (TCG) 73 bp Sc: 72.45 *F GACCGTGTGGCCTAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002708|AE002708.trna2-ArgTCG (1150447-1150519) Arg (TCG) 73 bp Sc: 72.45 *F GACCGTGTGGCCTAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAGT *F CCTGTCACGGTCG *F >gb|AE002593|AE002593.trna12-ArgTCG (3289037-3288965) Arg (TCG) 73 bp Sc: *F 74.10 *F GACCGTGTGGCCTAATGGATAAGGCGTCGGACTTCGGATCCGAAGATTGCAGGTTCGAAT *F CCTGTCACGGTCG *F >gb|AE002638|AE002638.trna12-ArgTCT (2755501-2755429) Arg (TCT) 73 bp Sc: *F 66.34 *F GGCCGTGTAGCCTAATGGATAAGGCGTCGGATTTCTGATCCGAAAATTGCGGGTTCAAGT *F CCCGTCATGGTCG *F >gb|AE002638|AE002638.trna8-ArgTCT (2968898-2968826) Arg (TCT) 73 bp Sc: 74.52 *F GACCCTTTAGCGCATTGGATAGCGCGTTGGACTTCTAATCCAAAGGTGGCGGGTTCGATT *F CCCGCAAGGGTTG *F >gb|AE002787|AE002787.trna23-ArgTCT (9064567-9064639) Arg (TCT) 73 bp Sc: *F 82.67 *F GTCCCTTTGGCGCAGAGGATAGCGCGTTGGACTTCTAATCCAAAGGTCGCGGGTTCGATC *F CCCGCAAGGGATG *F >gb|AE002769|AE002769.trna18-AsnGTT (66305-66232) Asn (GTT) 74 bp Sc: 76.75 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGGC *F >gb|AE002769|AE002769.trna13-AsnGTT (94158-94085) Asn (GTT) 74 bp Sc: 83.77 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAGAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002575|AE002575.trna5-AsnGTT (3594348-3594421) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002708|AE002708.trna67-AsnGTT (425185-425112) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002769|AE002769.trna1-AsnGTT (66475-66548) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002769|AE002769.trna11-AsnGTT (103580-103507) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002769|AE002769.trna6-AsnGTT (94670-94743) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002769|AE002769.trna7-AsnGTT (103187-103260) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002769|AE002769.trna8-AsnGTT (104090-104163) Asn (GTT) 74 bp Sc: 86.74 *F GCCTCCGTGGCGCAATTGGTTAGCGCGTTCGGCTGTTAACCGAAAGGTTGGTGGTTCGAG *F TCCACCCGGGGGCG *F >gb|AE002690|AE002690.trna22-AspGTC (3529657-3529585) Asp (GTC) 73 bp Sc: *F 60.88 *F CCTCCGATAGTATAGTGGTTAAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATT *F CCCCGTCGGGGAG *F >gb|AE002708|AE002708.trna36-AspGTC (17130623-17130552) Asp (GTC) 72 bp Sc: *F 68.81 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCTGGGAG *F >gb|AE002602|AE002602.trna20-AspGTC (7015387-7015316) Asp (GTC) 72 bp Sc: *F 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002690|AE002690.trna21-AspGTC (3731236-3731165) Asp (GTC) 72 bp Sc: *F 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002690|AE002690.trna23-AspGTC (298889-298818) Asp (GTC) 72 bp Sc: 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002690|AE002690.trna4-AspGTC (3529278-3529349) Asp (GTC) 72 bp Sc: 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002690|AE002690.trna6-AspGTC (3667385-3667456) Asp (GTC) 72 bp Sc: 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002690|AE002690.trna7-AspGTC (3726475-3726546) Asp (GTC) 72 bp Sc: 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002690|AE002690.trna8-AspGTC (3730810-3730881) Asp (GTC) 72 bp Sc: 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002708|AE002708.trna30-AspGTC (16770750-16770821) Asp (GTC) 72 bp Sc: *F 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002708|AE002708.trna31-AspGTC (17130279-17130350) Asp (GTC) 72 bp Sc: *F 75.34 *F TCCTCGATAGTATAGTGGTTAGTATCCCCGCCTGTCACGCGGGAGACCGGGGTTCAATTC *F CCCGTCGGGGAG *F >gb|AE002647|AE002647.trna3-CysGCA (1512550-1512479) Cys (GCA) 72 bp Sc: 70.54 *F GGGGGTATAGCTCAGGGGCAGAGCATTCGACTGCAGATCGAGAGGTCCCCGGTTCAAATC *F CGGGTGCCCCCT *F >gb|AE002584|AE002584.trna13-CysGCA (2248908-2248979) Cys (GCA) 72 bp Sc: *F 74.46 *F GGGGGTATAGCTCAGGGGTAGAGCATTCGACTGCAGATCGAGAGGTCCCCGGTTCAAATC *F CGGGTGCCCCCT *F >gb|AE002584|AE002584.trna12-CysGCA (1904808-1904879) Cys (GCA) 72 bp Sc: *F 75.79 *F GGGGGCATAGCTCAGGGGTAGAGCGTTCGACTGCAGATCGACAGGTCCCTGGTTCAAATC *F CGGGTGCCCCCT *F >gb|AE002584|AE002584.trna14-CysGCA (3069999-3070070) Cys (GCA) 72 bp Sc: *F 75.91 *F GGGGATATAGCTCAGTGGTAGAGCATTCGACTGCAGATCGAGAGGTCCCCGGTTCAAACC *F CGGGTGTCCCCT *F >gb|AE002584|AE002584.trna15-CysGCA (3070253-3070324) Cys (GCA) 72 bp Sc: *F 75.91 *F GGGGATATAGCTCAGTGGTAGAGCATTCGACTGCAGATCGAGAGGTCCCCGGTTCAAACC *F CGGGTGTCCCCT *F >gb|AE002584|AE002584.trna16-CysGCA (3070631-3070702) Cys (GCA) 72 bp Sc: *F 75.91 *F GGGGATATAGCTCAGTGGTAGAGCATTCGACTGCAGATCGAGAGGTCCCCGGTTCAAACC *F CGGGTGTCCCCT *F >gb|AE002584|AE002584.trna18-CysGCA (3071781-3071852) Cys (GCA) 72 bp Sc: *F 75.91 *F GGGGATATAGCTCAGTGGTAGAGCATTCGACTGCAGATCGAGAGGTCCCCGGTTCAAACC *F CGGGTGTCCCCT *F >gb|AE002690|AE002690.trna19-GlnCTG (9037163-9037092) Gln (CTG) 72 bp Sc: *F 69.04 *F GGTTCTATGGTGTAATGGTTAGCACTTTGGACTCTGAATCCAGCGATCCGAGTTCAAATC *F TCGGTAGAACCT *F >gb|AE002638|AE002638.trna5-GlnCTG (2923384-2923455) Gln (CTG) 72 bp Sc: 71.12 *F GGTTCTATGGTGTAATGGTTAGCACTCAGGACTCTGAATCCTGCGATCCGAGTTCAAATC *F TCGGTAGAACCT *F >gb|AE002566|AE002566.trna1-GlnCTG (3630061-3630132) Gln (CTG) 72 bp Sc: 72.37 *F GGTTCCATGGTGTAATGGTTAGCACTCAGGACTCTGAATCCTGCGATCCGAGTTCAAATC *F TCGGTGGAACCT *F >gb|AE002638|AE002638.trna10-GlnCTG (2924786-2924715) Gln (CTG) 72 bp Sc: *F 72.37 *F GGTTCCATGGTGTAATGGTTAGCACTCAGGACTCTGAATCCTGCGATCCGAGTTCAAATC *F TCGGTGGAACCT *F >gb|AE002638|AE002638.trna4-GlnCTG (2921074-2921145) Gln (CTG) 72 bp Sc: 72.37 *F GGTTCCATGGTGTAATGGTTAGCACTCAGGACTCTGAATCCTGCGATCCGAGTTCAAATC *F TCGGTGGAACCT *F >gb|AE002638|AE002638.trna6-GlnCTG (2923607-2923678) Gln (CTG) 72 bp Sc: 72.37 *F GGTTCCATGGTGTAATGGTTAGCACTCAGGACTCTGAATCCTGCGATCCGAGTTCAAATC *F TCGGTGGAACCT *F >gb|AE002638|AE002638.trna9-GlnCTG (2925169-2925098) Gln (CTG) 72 bp Sc: 72.37 *F GGTTCCATGGTGTAATGGTTAGCACTCAGGACTCTGAATCCTGCGATCCGAGTTCAAATC *F TCGGTGGAACCT *F >gb|AE002787|AE002787.trna14-GlnCTG (4533329-4533400) Gln (CTG) 72 bp Sc: *F 73.65 *F GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCGATCCGAGTTCAAATC *F TCGGTGGAACCT *F >gb|AE002566|AE002566.trna6-GlnTTG (3181126-3181055) Gln (TTG) 72 bp Sc: 69.58 *F GGTTCTATGGTGTAACGGTTAGCACTCTGGACTTTGAATCCAGCGATCCGAGTTCAAATC *F TCGGTAGAACCT *F >gb|AE002602|AE002602.trna4-GlnTTG (8462145-8462216) Gln (TTG) 72 bp Sc: 72.03 *F GGTTCTATGGTGTAATGGTTAGCACTCTGGACTTTGAATCCAGCGATCCGAGTTCAAATC *F TCGGTAGAACCT *F >gb|AE002708|AE002708.trna34-GlnTTG (19158558-19158487) Gln (TTG) 72 bp Sc: *F 72.03 *F GGTTCTATGGTGTAATGGTTAGCACTCTGGACTTTGAATCCAGCGATCCGAGTTCAAATC *F TCGGTAGAACCT *F >gb|AE002708|AE002708.trna35-GlnTTG (19158309-19158238) Gln (TTG) 72 bp Sc: *F 72.03 *F GGTTCTATGGTGTAATGGTTAGCACTCTGGACTTTGAATCCAGCGATCCGAGTTCAAATC *F TCGGTAGAACCT *F >gb|AE002584|AE002584.trna10-GluCTC (1380635-1380706) Glu (CTC) 72 bp Sc: *F 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna11-GluCTC (1380976-1381047) Glu (CTC) 72 bp Sc: *F 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna3-GluCTC (1359129-1359200) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna4-GluCTC (1359433-1359504) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna5-GluCTC (1359724-1359795) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna6-GluCTC (1376890-1376961) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna7-GluCTC (1377127-1377198) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna8-GluCTC (1377364-1377435) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002584|AE002584.trna9-GluCTC (1377601-1377672) Glu (CTC) 72 bp Sc: 77.51 *F TCCCATATTGTCTAGTGGTTAGGATATCCGGCTCTCACCCGGAAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002647|AE002647.trna2-GluCTC (2498612-2498541) Glu (CTC) 72 bp Sc: 77.90 *F TCCCATATTGTCTAGTGGTTAGGATACCCGGCTCTCACCCGGGAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna3-GluCTC (1027003-1027074) Glu (CTC) 72 bp Sc: 77.90 *F TCCCATATTGTCTAGTGGTTAGGATACCCGGCTCTCACCCGGGAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna55-GluCTC (1026375-1026304) Glu (CTC) 72 bp Sc: *F 77.90 *F TCCCATATTGTCTAGTGGTTAGGATACCCGGCTCTCACCCGGGAGGCCCGGGTTCAATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna4-GluTTC (1027210-1027281) Glu (TTC) 72 bp Sc: 80.27 *F TCCCATATGGTCTAGTGGCTAGGATATCTGGCTTTCACCCAGAAGGCCCGGGTTCGATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna43-GluTTC (4521343-4521272) Glu (TTC) 72 bp Sc: *F 80.27 *F TCCCATATGGTCTAGTGGCTAGGATATCTGGCTTTCACCCAGAAGGCCCGGGTTCGATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna5-GluTTC (1027731-1027802) Glu (TTC) 72 bp Sc: 80.27 *F TCCCATATGGTCTAGTGGCTAGGATATCTGGCTTTCACCCAGAAGGCCCGGGTTCGATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna6-GluTTC (1029049-1029120) Glu (TTC) 72 bp Sc: 80.27 *F TCCCATATGGTCTAGTGGCTAGGATATCTGGCTTTCACCCAGAAGGCCCGGGTTCGATTC *F CCGGTATGGGAA *F >gb|AE002787|AE002787.trna8-GluTTC (1189234-1189305) Glu (TTC) 72 bp Sc: 80.27 *F TCCCATATGGTCTAGTGGCTAGGATATCTGGCTTTCACCCAGAAGGCCCGGGTTCGATTC *F CCGGTATGGGAA *F >gb|AE002690|AE002690.trna15-GlyGCC (9557054-9556984) Gly (GCC) 71 bp Sc: *F 77.95 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCTGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002575|AE002575.trna13-GlyGCC (380126-380056) Gly (GCC) 71 bp Sc: 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002575|AE002575.trna3-GlyGCC (401556-401626) Gly (GCC) 71 bp Sc: 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002638|AE002638.trna11-GlyGCC (2902631-2902561) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002638|AE002638.trna7-GlyGCC (2996165-2996235) Gly (GCC) 71 bp Sc: 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002690|AE002690.trna10-GlyGCC (9544221-9544291) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002690|AE002690.trna16-GlyGCC (9556746-9556676) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002690|AE002690.trna17-GlyGCC (9544821-9544751) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002690|AE002690.trna18-GlyGCC (9544507-9544437) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002690|AE002690.trna2-GlyGCC (2909049-2909119) Gly (GCC) 71 bp Sc: 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002787|AE002787.trna10-GlyGCC (2114387-2114457) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002787|AE002787.trna44-GlyGCC (3774022-3773952) Gly (GCC) 71 bp Sc: *F 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002787|AE002787.trna58-GlyGCC (550417-550347) Gly (GCC) 71 bp Sc: 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002787|AE002787.trna59-GlyGCC (547101-547031) Gly (GCC) 71 bp Sc: 82.25 *F GCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCC *F CGGCCGATGCA *F >gb|AE002575|AE002575.trna10-GlyTCC (1067996-1067925) Gly (TCC) 72 bp Sc: *F 72.21 *F GCGTCGGTGGTGTAATGGTCAGCATAGTTGCCTTCCAAGCAGTTGATCCGGGTTCGATTC *F CCGGCCGACGCA *F >gb|AE002575|AE002575.trna11-GlyTCC (1062686-1062615) Gly (TCC) 72 bp Sc: *F 72.21 *F GCGTCGGTGGTGTAATGGTCAGCATAGTTGCCTTCCAAGCAGTTGATCCGGGTTCGATTC *F CCGGCCGACGCA *F >gb|AE002699|AE002699.trna11-GlyTCC (566891-566820) Gly (TCC) 72 bp Sc: 76.96 *F GCGTCGGTGGTGTAATGGTTAGCATAGTTGCCTTCCAAGCAGTTGACCCGGGTTCGATTC *F CCGGCCGACGCA *F >gb|AE002699|AE002699.trna3-GlyTCC (565737-565808) Gly (TCC) 72 bp Sc: 76.96 *F GCGTCGGTGGTGTAATGGTTAGCATAGTTGCCTTCCAAGCAGTTGACCCGGGTTCGATTC *F CCGGCCGACGCA *F >gb|AE002708|AE002708.trna18-GlyTCC (10343027-10343098) Gly (TCC) 72 bp Sc: *F 76.96 *F GCGTCGGTGGTGTAATGGTTAGCATAGTTGCCTTCCAAGCAGTTGACCCGGGTTCGATTC *F CCGGCCGACGCA *F >gb|AE002708|AE002708.trna19-GlyTCC (10343383-10343454) Gly (TCC) 72 bp Sc: *F 76.96 *F GCGTCGGTGGTGTAATGGTTAGCATAGTTGCCTTCCAAGCAGTTGACCCGGGTTCGATTC *F CCGGCCGACGCA *F >gb|AE002787|AE002787.trna19-HisGTG (8381977-8382048) His (GTG) 72 bp Sc: *F 67.15 *F GCCGTGATCGTCTAGTGGTTAGGACCCCACGTTGTGGCCGTGGTAACCCAGGTTCGAATC *F CTGGTCACGGCA *F >gb|AE002787|AE002787.trna20-HisGTG (8382738-8382809) His (GTG) 72 bp Sc: *F 67.15 *F GCCGTGATCGTCTAGTGGTTAGGACCCCACGTTGTGGCCGTGGTAACCCAGGTTCGAATC *F CTGGTCACGGCA *F >gb|AE002787|AE002787.trna21-HisGTG (8383046-8383117) His (GTG) 72 bp Sc: *F 67.15 *F GCCGTGATCGTCTAGTGGTTAGGACCCCACGTTGTGGCCGTGGTAACCCAGGTTCGAATC *F CTGGTCACGGCA *F >gb|AE002787|AE002787.trna35-HisGTG (8380625-8380554) His (GTG) 72 bp Sc: *F 67.15 *F GCCGTGATCGTCTAGTGGTTAGGACCCCACGTTGTGGCCGTGGTAACCCAGGTTCGAATC *F CTGGTCACGGCA *F >gb|AE002787|AE002787.trna9-HisGTG (1247768-1247839) His (GTG) 72 bp Sc: 67.15 *F GCCGTGATCGTCTAGTGGTTAGGACCCCACGTTGTGGCCGTGGTAACCCAGGTTCGAATC *F CTGGTCACGGCA *F >gb|AE002769|AE002769.trna15-IleAAT (90526-90453) Ile (AAT) 74 bp Sc: 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna18-IleAAT (7289268-7289341) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna29-IleAAT (12523500-12523427) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna37-IleAAT (7288639-7288566) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna38-IleAAT (7288338-7288265) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna39-IleAAT (7287846-7287773) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna40-IleAAT (7287530-7287457) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna42-IleAAT (7281754-7281681) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002787|AE002787.trna53-IleAAT (1039550-1039477) Ile (AAT) 74 bp Sc: *F 82.79 *F GGCCCATTAGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCGAT *F CCCCTCATGGGCCA *F >gb|AE002584|AE002584.trna1-IleTAT (750630-750727) Ile (TAT) 98 bp Sc: 65.59 *F GCTCCAGTGGCGCAATTGGTTAGCGCACGGTACTTATAATCAGTATTCTGTGTGTATGAG *F CTATGCCGGGGTTGTGAGTTCGAGCCTCACCTGGAGCA *F >gb|AE002584|AE002584.trna2-IleTAT (751490-751589) Ile (TAT) 100 bp Sc: 65.99 *F GCTCCAGTGGCGCAATTGGTTAGCGCACGGTACTTATAATCAGTATTCTGTGTGTATATG *F AGCTATGCCGGGGTTGTGAGTTCGAGCCTCACCTGGAGCA *F >gb|AE002602|AE002602.trna21-LeuAAG (6985166-6985085) Leu (AAG) 82 bp Sc: *F 71.48 *F GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTAAGGCACCAGTCTCCTCGGAGGCGTG *F GGTTCGAATCCCACCGCTGCCA *F >gb|AE002787|AE002787.trna13-LeuAAG (3597984-3598065) Leu (AAG) 82 bp Sc: *F 71.48 *F GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTAAGGCACCAGTCTCCTCGGAGGCGTG *F GGTTCGAATCCCACCGCTGCCA *F >gb|AE002787|AE002787.trna15-LeuAAG (5753326-5753407) Leu (AAG) 82 bp Sc: *F 71.48 *F GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTAAGGCACCAGTCTCCTCGGAGGCGTG *F GGTTCGAATCCCACCGCTGCCA *F >gb|AE002787|AE002787.trna45-LeuAAG (3596730-3596649) Leu (AAG) 82 bp Sc: *F 71.48 *F GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTAAGGCACCAGTCTCCTCGGAGGCGTG *F GGTTCGAATCCCACCGCTGCCA *F >gb|AE002787|AE002787.trna49-LeuAAG (1984053-1983972) Leu (AAG) 82 bp Sc: *F 71.48 *F GGTAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTAAGGCACCAGTCTCCTCGGAGGCGTG *F GGTTCGAATCCCACCGCTGCCA *F >gb|AE002708|AE002708.trna37-LeuCAA (15816611-15816489) Leu (CAA) 123 bp Sc: *F 65.38 *F GTCAGGATGGCCGAGCGGTCTAAGGCGCCAGACTCAAGGCATTCAGTCTTGCCCTTCGCA *F GAAGGGCGTGTACGAGCGTTCTGGTCCTCTCTGAGGGCGTGGGTTCGAATCCCACTTCTG *F ACA *F >gb|AE002602|AE002602.trna19-LeuCAA (7050825-7050701) Leu (CAA) 125 bp Sc: *F 68.33 *F GTCAGGATGGCCGAGCGGTCTAAGGCGCCAGACTCAAGAGCGAAAGTCTCTTACCTTTCA *F CAGCAAGGTTCGATTGAGCGTTCTGGTCCTCTCTGAGGGCGTGGGTTCGAATCCCACTTC *F TGACA *F >gb|AE002787|AE002787.trna41-LeuCAA (7286955-7286829) Leu (CAA) 127 bp Sc: *F 72.67 *F GTCAGGATGGCCGAGCGGTCTAAGGCGCCAGACTCAAGATTGAAAATCTTACTTTCTGAA *F CGAAAGTGTTTGTGAATGAGCGTTCTGGTCCTCTTTGAGGGCGTGGGTTCGAATCCCACT *F TCTGACA *F >gb|AE002787|AE002787.trna17-LeuCAA (7288808-7288928) Leu (CAA) 121 bp Sc: *F 72.14 *F GTCAGGATGGCCGAGCGGTCTAAGGCGCCAGACTCAAGATTTAAAATCTTACTTTCTGAA *F CGAAAGCGTATGAGCGTTCTGGTCCTCTCTGAGGGCGTGGGTTCGAATCCCACTTCTGAC *F A *F >gb|AE002602|AE002602.trna10-LeuCAG (12197824-12197906) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002602|AE002602.trna5-LeuCAG (12189830-12189912) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002602|AE002602.trna6-LeuCAG (12192044-12192126) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002602|AE002602.trna7-LeuCAG (12192395-12192477) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002602|AE002602.trna8-LeuCAG (12197364-12197446) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002602|AE002602.trna9-LeuCAG (12197587-12197669) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002647|AE002647.trna1-LeuCAG (2494080-2494162) Leu (CAG) 83 bp Sc: 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002787|AE002787.trna30-LeuCAG (11998761-11998679) Leu (CAG) 83 bp Sc: *F 77.88 *F GTCAGGATGGCCGAGTGGTCTAAGGCGCTGCGTTCAGGTCGCAGTCTACTCTGTAGGCGT *F GGGTTCGAATCCCACTTCTGACA *F >gb|AE002708|AE002708.trna29-LeuTAA (16480680-16480763) Leu (TAA) 84 bp Sc: *F 68.33 *F GTCAGGTTGGCCGAGCGGTCTAAGGCGCCAGATTTAAGCTCTGGTTCTCGAGAGGGAGCG *F TGGGTTCGAACCCCACACCTGACA *F >gb|AE002690|AE002690.trna13-LeuTAA (10156452-10156369) Leu (TAA) 84 bp Sc: *F 68.86 *F GCCAGGTTGGCCGAGCGGTCTAAGGCGCCAGATTTAAGCTCTGGTTCTCGAGAGAGAGCG *F TGGGTTCGAGTCCCACACCTGGCA *F >gb|AE002708|AE002708.trna28-LeuTAA (16328484-16328567) Leu (TAA) 84 bp Sc: *F 69.13 *F GCCAGGTTGGCCGAGCGGTCTAAGGCGCCAGATTTAAGCTCTGGTTCTCGAGAGAGAGCG *F TGGGTTCGAACCCCACACCTGGCA *F >gb|AE002708|AE002708.trna40-LeuTAA (12830608-12830525) Leu (TAA) 84 bp Sc: *F 70.06 *F GCCAGGTTGGCCGAGCGGTCTAAGGCGCCAGATTTAAGCTCTGGTTCTCGTGAGAGAGCG *F TGGGTTCGAACCCCACACCTGGCA *F >gb|AE002708|AE002708.trna32-LeuTAG (22437471-22437392) Leu (TAG) 80 bp Sc: *F 69.19 *F GGCAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTTAGGCACCAGTCCGAAAGGGCGTGGG *F TTCGAATCCCACCGCTGTCA *F >gb|AE002708|AE002708.trna33-LeuTAG (22436819-22436740) Leu (TAG) 80 bp Sc: *F 69.19 *F GGCAGCGTGGCCGAGCGGTCTAAGGCGCTGGTTTTAGGCACCAGTCCGAAAGGGCGTGGG *F TTCGAATCCCACCGCTGTCA *F >gb|AE002584|AE002584.trna17-LysCTT (3070866-3070938) Lys (CTT) 73 bp Sc: *F 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002584|AE002584.trna20-LysCTT (3071225-3071153) Lys (CTT) 73 bp Sc: *F 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002769|AE002769.trna14-LysCTT (90693-90621) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002769|AE002769.trna3-LysCTT (85109-85181) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002769|AE002769.trna4-LysCTT (91729-91801) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002769|AE002769.trna5-LysCTT (93924-93996) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002769|AE002769.trna9-LysCTT (114322-114394) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002778|AE002778.trna1-LysCTT (457256-457184) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002778|AE002778.trna2-LysCTT (456407-456335) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002778|AE002778.trna3-LysCTT (455861-455789) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002778|AE002778.trna4-LysCTT (454931-454859) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002787|AE002787.trna16-LysCTT (7183504-7183576) Lys (CTT) 73 bp Sc: *F 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002787|AE002787.trna56-LysCTT (978050-977978) Lys (CTT) 73 bp Sc: 80.47 *F GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTCAGGGTCGTGGGTTCGAGC *F CCCACGTTGGGCG *F >gb|AE002699|AE002699.trna5-LysTTT (876900-876972) Lys (TTT) 73 bp Sc: 76.87 *F CCCCGGATAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGTTCGGGCG *F >gb|AE002699|AE002699.trna10-LysTTT (875844-875772) Lys (TTT) 73 bp Sc: 84.31 *F GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGTTCGGGCG *F >gb|AE002699|AE002699.trna4-LysTTT (874598-874670) Lys (TTT) 73 bp Sc: 84.31 *F GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGTTCGGGCG *F >gb|AE002699|AE002699.trna8-LysTTT (878146-878074) Lys (TTT) 73 bp Sc: 84.31 *F GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGTTCGGGCG *F >gb|AE002699|AE002699.trna9-LysTTT (876315-876243) Lys (TTT) 73 bp Sc: 84.31 *F GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGTTCGGGCG *F >gb|AE002708|AE002708.trna3-LysTTT (4398969-4399041) Lys (TTT) 73 bp Sc: 84.31 *F GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGTTCGGGCG *F >gb|AE002690|AE002690.trna3-LysTTT (3330035-3330107) Lys (TTT) 73 bp Sc: 84.71 *F GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTTTTAATCCAAGGGTCCAGGGTTCAAGT *F CCCTGCTCGGGCG *F >gb|AE002787|AE002787.trna31-MetCAT (11028819-11028748) Met (CAT) 72 bp Sc: *F 66.24 *F AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCATAACTCAGAGGTCCGAGGATCGAAAC *F CTTGCTCTGCTA *F >gb|AE002584|AE002584.trna21-MetCAT (724921-724850) Met (CAT) 72 bp Sc: 70.82 *F AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCATAACCCAGAGGTCCGAGGATCGAAAC *F CTTGCTCTGCTA *F >gb|AE002602|AE002602.trna2-MetCAT (5609856-5609927) Met (CAT) 72 bp Sc: 70.82 *F AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCATAACCCAGAGGTCCGAGGATCGAAAC *F CTTGCTCTGCTA *F >gb|AE002602|AE002602.trna23-MetCAT (5609525-5609454) Met (CAT) 72 bp Sc: *F 70.82 *F AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCATAACCCAGAGGTCCGAGGATCGAAAC *F CTTGCTCTGCTA *F >gb|AE002787|AE002787.trna54-MetCAT (1029357-1029286) Met (CAT) 72 bp Sc: *F 70.82 *F AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCATAACCCAGAGGTCCGAGGATCGAAAC *F CTTGCTCTGCTA *F >gb|AE002787|AE002787.trna7-MetCAT (1029571-1029642) Met (CAT) 72 bp Sc: 70.82 *F AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCATAACCCAGAGGTCCGAGGATCGAAAC *F CTTGCTCTGCTA *F >gb|AE002584|AE002584.trna19-MetCAT (3096276-3096204) Met (CAT) 73 bp Sc: *F 79.03 *F GCCTCGATGGCGCAGTTGGCAGCGCGTAAGTCTCATAATCTTAAGGTCGTGAGTTCGAGC *F CTCACTCGGGGCA *F >gb|AE002602|AE002602.trna24-MetCAT (4057326-4057254) Met (CAT) 73 bp Sc: *F 79.43 *F GCCTCGGTGGCGCAGTTGGCAGCGCGTAAGTCTCATAATCTTAAGGTCGTGAGTTCGAGC *F CTCACCCGGGGCA *F >gb|AE002602|AE002602.trna25-MetCAT (3929593-3929521) Met (CAT) 73 bp Sc: *F 79.43 *F GCCTCGGTGGCGCAGTTGGCAGCGCGTAAGTCTCATAATCTTAAGGTCGTGAGTTCGAGC *F CTCACCCGGGGCA *F >gb|AE002699|AE002699.trna7-MetCAT (1201937-1202009) Met (CAT) 73 bp Sc: 79.43 *F GCCTCGGTGGCGCAGTTGGCAGCGCGTAAGTCTCATAATCTTAAGGTCGTGAGTTCGAGC *F CTCACCCGGGGCA *F >gb|AE002787|AE002787.trna22-MetCAT (9056371-9056443) Met (CAT) 73 bp Sc: *F 79.43 *F GCCTCGGTGGCGCAGTTGGCAGCGCGTAAGTCTCATAATCTTAAGGTCGTGAGTTCGAGC *F CTCACCCGGGGCA *F >gb|AE002787|AE002787.trna34-MetCAT (9064357-9064285) Met (CAT) 73 bp Sc: *F 79.43 *F GCCTCGGTGGCGCAGTTGGCAGCGCGTAAGTCTCATAATCTTAAGGTCGTGAGTTCGAGC *F CTCACCCGGGGCA *F >gb|AE002708|AE002708.trna54-PheGAA (6029582-6029510) Phe (GAA) 73 bp Sc: *F 75.08 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGNN *F >gb|AE002566|AE002566.trna4-PheGAA (6325411-6325483) Phe (GAA) 73 bp Sc: 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002566|AE002566.trna5-PheGAA (6552588-6552516) Phe (GAA) 73 bp Sc: 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002690|AE002690.trna5-PheGAA (3618394-3618466) Phe (GAA) 73 bp Sc: 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002708|AE002708.trna5-PheGAA (6029215-6029287) Phe (GAA) 73 bp Sc: 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002708|AE002708.trna6-PheGAA (8554738-8554810) Phe (GAA) 73 bp Sc: 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002787|AE002787.trna11-PheGAA (3138166-3138238) Phe (GAA) 73 bp Sc: *F 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002787|AE002787.trna47-PheGAA (3138002-3137930) Phe (GAA) 73 bp Sc: *F 82.98 *F GCCGAAATAGCTCAGTTGGGAGAGCGTTAGACTGAAGATCTAAAGGTCCCCGGTTCAATC *F CCGGGTTTCGGCA *F >gb|AE002602|AE002602.trna16-ProAGG (7053720-7053649) Pro (AGG) 72 bp Sc: *F 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002602|AE002602.trna17-ProAGG (7053383-7053312) Pro (AGG) 72 bp Sc: *F 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002602|AE002602.trna18-ProAGG (7052569-7052498) Pro (AGG) 72 bp Sc: *F 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002690|AE002690.trna11-ProAGG (9624987-9625058) Pro (AGG) 72 bp Sc: *F 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002690|AE002690.trna12-ProAGG (9625802-9625873) Pro (AGG) 72 bp Sc: *F 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002690|AE002690.trna14-ProAGG (9625595-9625524) Pro (AGG) 72 bp Sc: *F 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002690|AE002690.trna9-ProAGG (9522008-9522079) Pro (AGG) 72 bp Sc: 72.31 *F GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTAGGGTGCGGGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002566|AE002566.trna2-ProCGG (3637984-3638055) Pro (CGG) 72 bp Sc: 72.94 *F GGCTCGTTGGTCTAGGGGTATGATTTTCGCTTCGGGTGCGAGAGGTCCCGGGTTCAATTC *F CCGGACGAGCCC *F >gb|AE002593|AE002593.trna10-ProCGG (7738243-7738314) Pro (CGG) 72 bp Sc: *F 75.20 *F GGCTCGTTGGTCTAGAGGTATGATTCTCGCTTCGGGTGCGAGAGGTCCCGGGTTCAATTC *F CCGGACGAGCCC *F >gb|AE002602|AE002602.trna1-ProCGG (2941402-2941473) Pro (CGG) 72 bp Sc: 75.20 *F GGCTCGTTGGTCTAGAGGTATGATTCTCGCTTCGGGTGCGAGAGGTCCCGGGTTCAATTC *F CCGGACGAGCCC *F >gb|AE002602|AE002602.trna26-ProCGG (1674504-1674433) Pro (CGG) 72 bp Sc: *F 75.20 *F GGCTCGTTGGTCTAGAGGTATGATTCTCGCTTCGGGTGCGAGAGGTCCCGGGTTCAATTC *F CCGGACGAGCCC *F >gi|7289277|gb|AE002611.1|AE002611.trna1-ProCGG (67042-67113) Pro (CGG) 72 bp *F Sc: 76.52 *F GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTCGGGTGCGAGAGGTCCCGGGTTCAAATC *F CCGGACGAGCCC *F >gb|AE002708|AE002708.trna11-ProTGG (9853436-9853507) Pro (TGG) 72 bp Sc: *F 75.74 *F GGCTCAATGGTCTAGGGGTATGATTCTCGCTTTGGGTGCGAGAGGTCCCGGGTTCAAATC *F CCGGTTGAGCCC *F >gb|AE002708|AE002708.trna13-ProTGG (9865154-9865225) Pro (TGG) 72 bp Sc: *F 75.74 *F GGCTCAATGGTCTAGGGGTATGATTCTCGCTTTGGGTGCGAGAGGTCCCGGGTTCAAATC *F CCGGTTGAGCCC *F >gb|AE002708|AE002708.trna14-ProTGG (9879085-9879156) Pro (TGG) 72 bp Sc: *F 75.74 *F GGCTCAATGGTCTAGGGGTATGATTCTCGCTTTGGGTGCGAGAGGTCCCGGGTTCAAATC *F CCGGTTGAGCCC *F >gb|AE002708|AE002708.trna42-ProTGG (10862802-10862731) Pro (TGG) 72 bp Sc: *F 75.74 *F GGCTCAATGGTCTAGGGGTATGATTCTCGCTTTGGGTGCGAGAGGTCCCGGGTTCAAATC *F CCGGTTGAGCCC *F >gb|AE002708|AE002708.trna51-ProTGG (9851656-9851585) Pro (TGG) 72 bp Sc: *F 75.74 *F GGCTCAATGGTCTAGGGGTATGATTCTCGCTTTGGGTGCGAGAGGTCCCGGGTTCAAATC *F CCGGTTGAGCCC *F >gb|AE002620|AE002620.trna2-IleAAT (289667-289741) Ile (AAT) 75 bp Sc: 44.17 *F Pseudo *F GACCCATTTGCTCAGTTGGTTAGAGCGTCGTGCTAATAACGCGAAGGTCGCGGGTTCAAA *F ATCCCCTCATTGTCG *F >gb|AE002602|AE002602.trna15-ThrAGT (11356075-11356002) Thr (AGT) 74 bp Sc: *F 52.51 Pseudo *F GGCGCCGTGGCTTAGTTGGGCAAAGCGCCTGTCTAGTAAACAGGAGATCGTAAGTTCGAA *F TCTCATCGGGGGTT *F >gb|AE002566|AE002566.trna3-GluCTC (5641089-5641159) Glu (CTC) 71 bp Sc: *F 30.14 Pseudo *F CTTTGCGTAGCTTAGGGGTTAGAGCATTCGACTCTCGTTTGAAAGTGAACGGGTTCAAAT *F CCCAGTAGGGT *F >gb|AE002699|AE002699.trna6-LysCTT (878702-878774) Lys (CTT) 73 bp Sc: 28.65 *F Pseudo *F GCTTGAATAGCTTAGTTGGTAAAGCGTTGGATTCTTAAATCATTGGTACACGGTTCAAGT *F TCATGCTCAGATG *F >gb|AE002787|AE002787.trna26-SeC(e)TCA (9359186-9359271) SeC(e) (TCA) 86 bp *F Sc: 56.66 *F GCCCCACTGAACTTCGGTGGTCCGGGGTGCGGACTTCAAATCCGTAGTCGATTTGCGTCG *F AAGTGGTTCGATTCCACCTGGGGGGC *F >gb|AE002593|AE002593.trna15-SerAGA (3221617-3221536) Ser (AGA) 82 bp Sc: *F 83.36 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002593|AE002593.trna9-SerAGA (3289895-3289976) Ser (AGA) 82 bp Sc: 83.36 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002593|AE002593.trna11-SerAGA (3303326-3303245) Ser (AGA) 82 bp Sc: *F 84.54 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGACTGCG *F >gb|AE002593|AE002593.trna14-SerAGA (3222074-3221993) Ser (AGA) 82 bp Sc: *F 84.54 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGACTGCG *F >gb|AE002602|AE002602.trna13-SerAGA (14883881-14883800) Ser (AGA) 82 bp Sc: *F 84.54 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGACTGCG *F >gb|AE002638|AE002638.trna14-SerAGA (1739897-1739816) Ser (AGA) 82 bp Sc: *F 84.54 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGACTGCG *F >gb|AE002638|AE002638.trna2-SerAGA (1736395-1736476) Ser (AGA) 82 bp Sc: 84.54 *F GCAGTCGTGGCCGAGCGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGACTGCG *F >gb|AE002593|AE002593.trna8-SerAGA (3255817-3255898) Ser (AGA) 82 bp Sc: 85.81 *F GCAGTCGTGGCCGAGTGGTTAAGGCGTCTGACTAGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002593|AE002593.trna13-SerCGA (3288689-3288608) Ser (CGA) 82 bp Sc: *F 86.41 *F GCAGTCGTGGCCGAGTGGTTAAGGCGTCTGACTCGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002593|AE002593.trna2-SerCGA (3211472-3211553) Ser (CGA) 82 bp Sc: 86.41 *F GCAGTCGTGGCCGAGTGGTTAAGGCGTCTGACTCGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002593|AE002593.trna3-SerCGA (3211872-3211953) Ser (CGA) 82 bp Sc: 86.41 *F GCAGTCGTGGCCGAGTGGTTAAGGCGTCTGACTCGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002787|AE002787.trna50-SerCGA (1339580-1339499) Ser (CGA) 82 bp Sc: *F 86.41 *F GCAGTCGTGGCCGAGTGGTTAAGGCGTCTGACTCGAAATCAGATTCCCTCTGGGAGCGTA *F GGTTCGAATCCTACCGGCTGCG *F >gb|AE002708|AE002708.trna26-SerGCT (14612289-14612370) Ser (GCT) 82 bp Sc: *F 82.84 *F GACGAGGTGGCCGAGAGGTTAAGGCGTTGGACTGCTAATCCAATGTGCTCTGCACGCGTG *F GGTTCGAATCCCATCCTCGTCG *F >gb|AE002708|AE002708.trna27-SerGCT (14613334-14613415) Ser (GCT) 82 bp Sc: *F 82.84 *F GACGAGGTGGCCGAGAGGTTAAGGCGTTGGACTGCTAATCCAATGTGCTCTGCACGCGTG *F GGTTCGAATCCCATCCTCGTCG *F >gb|AE002708|AE002708.trna39-SerGCT (14612642-14612561) Ser (GCT) 82 bp Sc: *F 82.84 *F GACGAGGTGGCCGAGAGGTTAAGGCGTTGGACTGCTAATCCAATGTGCTCTGCACGCGTG *F GGTTCGAATCCCATCCTCGTCG *F >gb|AE002708|AE002708.trna53-SerGCT (6275877-6275796) Ser (GCT) 82 bp Sc: *F 82.84 *F GACGAGGTGGCCGAGAGGTTAAGGCGTTGGACTGCTAATCCAATGTGCTCTGCACGCGTG *F GGTTCGAATCCCATCCTCGTCG *F >gb|AE002708|AE002708.trna56-SerGCT (2547881-2547800) Ser (GCT) 82 bp Sc: *F 82.84 *F GACGAGGTGGCCGAGAGGTTAAGGCGTTGGACTGCTAATCCAATGTGCTCTGCACGCGTG *F GGTTCGAATCCCATCCTCGTCG *F >gb|AE002708|AE002708.trna38-SerGCT (14613023-14612942) Ser (GCT) 82 bp Sc: *F 87.38 *F GACGAGGTGGCCGAGTGGTTAAGGCGTTGGACTGCTAATCCAATGTGCTCTGCACGCGTG *F GGTTCGAATCCCATCCTCGTCG *F >gb|AE002575|AE002575.trna4-SerTGA (2292805-2292886) Ser (TGA) 82 bp Sc: 76.82 *F GTTGCGGTGTCCGAGTGGTTAAGGAGATGGACTTGAAATCCATTGGGTTCTACCCGCGCA *F GGTTCGAATCCTGTCCGCAGCG *F >gb|AE002575|AE002575.trna9-SerTGA (2292324-2292243) Ser (TGA) 82 bp Sc: 79.78 *F GCTGCGGTGTCCGAGTGGTTAAGGAGATGGACTTGAAATCCATTGGGTTCTACCCGCACA *F GGTTCAAGTCCTGTCCGCAGCG *F >gb|AE002787|AE002787.trna28-ThrAGT (13604305-13604378) Thr (AGT) 74 bp Sc: *F 69.29 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TTTCGCCGGGGCCT *F >gb|AE002690|AE002690.trna20-ThrAGT (4283294-4283221) Thr (AGT) 74 bp Sc: *F 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002708|AE002708.trna22-ThrAGT (12030225-12030298) Thr (AGT) 74 bp Sc: *F 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002708|AE002708.trna48-ThrAGT (9858354-9858281) Thr (AGT) 74 bp Sc: *F 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002708|AE002708.trna49-ThrAGT (9858113-9858040) Thr (AGT) 74 bp Sc: *F 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002708|AE002708.trna52-ThrAGT (8658449-8658376) Thr (AGT) 74 bp Sc: *F 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002708|AE002708.trna8-ThrAGT (8665329-8665402) Thr (AGT) 74 bp Sc: 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002787|AE002787.trna27-ThrAGT (13603515-13603588) Thr (AGT) 74 bp Sc: *F 75.61 *F GGCGCCGTGGCTTAGTTGGTTAAAGCGCCTGTCTAGTAAACAGGAGATCGTGAGTTCGAA *F TCTCGCCGGGGCCT *F >gb|AE002708|AE002708.trna23-ThrCGT (13116872-13116943) Thr (CGT) 72 bp Sc: *F 77.47 *F GCCTCTTTAGCTCAGTGGTAGAGCGTTGGTCTCGTAAACCAAAGGCCGTGAGTTCAACCC *F TCACAGGAGGCA *F >gb|AE002708|AE002708.trna24-ThrCGT (13117296-13117367) Thr (CGT) 72 bp Sc: *F 77.47 *F GCCTCTTTAGCTCAGTGGTAGAGCGTTGGTCTCGTAAACCAAAGGCCGTGAGTTCAACCC *F TCACAGGAGGCA *F >gb|AE002708|AE002708.trna25-ThrCGT (13117832-13117903) Thr (CGT) 72 bp Sc: *F 77.47 *F GCCTCTTTAGCTCAGTGGTAGAGCGTTGGTCTCGTAAACCAAAGGCCGTGAGTTCAACCC *F TCACAGGAGGCA *F >gb|AE002708|AE002708.trna4-ThrTGT (4474188-4474259) Thr (TGT) 72 bp Sc: 74.00 *F GCCTCTTTAGCTCAGTGGCAGAGCACTGGTCTTGTAAACCAGGGGCCGTGAGTTCAATTC *F TCACAAGAGGCA *F >gb|AE002708|AE002708.trna55-ThrTGT (4590246-4590175) Thr (TGT) 72 bp Sc: *F 76.08 *F GCCTCTTTAGCTCAGTGGCAGAGCACTGGTCTTGTAAACCAGGGGTCGTGAGTTCAATCC *F TCACAGGAGGCA *F >gb|AE002787|AE002787.trna24-ThrTGT (9311533-9311604) Thr (TGT) 72 bp Sc: *F 76.08 *F GCCTCTTTAGCTCAGTGGCAGAGCACTGGTCTTGTAAACCAGGGGTCGTGAGTTCAATCC *F TCACAGGAGGCA *F >gb|AE002787|AE002787.trna25-ThrTGT (9316995-9317066) Thr (TGT) 72 bp Sc: *F 76.08 *F GCCTCTTTAGCTCAGTGGCAGAGCACTGGTCTTGTAAACCAGGGGTCGTGAGTTCAATCC *F TCACAGGAGGCA *F >gb|AE002787|AE002787.trna32-ThrTGT (9322483-9322412) Thr (TGT) 72 bp Sc: *F 76.08 *F GCCTCTTTAGCTCAGTGGCAGAGCACTGGTCTTGTAAACCAGGGGTCGTGAGTTCAATCC *F TCACAGGAGGCA *F >gb|AE002787|AE002787.trna33-ThrTGT (9312137-9312066) Thr (TGT) 72 bp Sc: *F 76.08 *F GCCTCTTTAGCTCAGTGGCAGAGCACTGGTCTTGTAAACCAGGGGTCGTGAGTTCAATCC *F TCACAGGAGGCA *F >gb|AE002787|AE002787.trna1-TrpCCA (122202-122274) Trp (CCA) 73 bp Sc: 64.90 *F GACTCCGTGGCGCAACGGTAGCGCGTCTGACTCCAGATCAGAAGGTTGCGGTGTTCAAAT *F CACGTCGGGGTCA *F >gb|AE002787|AE002787.trna60-TrpCCA (122487-122416) Trp (CCA) 72 bp Sc: 75.41 *F GACTCCGTGGCGCAACGGTAGCGCGTCTGACTCCAGATCAGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002575|AE002575.trna1-TrpCCA (380226-380297) Trp (CCA) 72 bp Sc: 75.61 *F GACTCCGTGGCGCAACGGTAGCGCGTCCGACTCCAGATCGGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002575|AE002575.trna12-TrpCCA (401449-401378) Trp (CCA) 72 bp Sc: 75.61 *F GACTCCGTGGCGCAACGGTAGCGCGTCCGACTCCAGATCGGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002575|AE002575.trna14-TrpCCA (379834-379763) Trp (CCA) 72 bp Sc: 75.61 *F GACTCCGTGGCGCAACGGTAGCGCGTCCGACTCCAGATCGGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002575|AE002575.trna2-TrpCCA (399984-400055) Trp (CCA) 72 bp Sc: 75.61 *F GACTCCGTGGCGCAACGGTAGCGCGTCCGACTCCAGATCGGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002638|AE002638.trna1-TrpCCA (524380-524451) Trp (CCA) 72 bp Sc: 75.61 *F GACTCCGTGGCGCAACGGTAGCGCGTCCGACTCCAGATCGGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002787|AE002787.trna48-TrpCCA (2114626-2114555) Trp (CCA) 72 bp Sc: *F 75.61 *F GACTCCGTGGCGCAACGGTAGCGCGTCCGACTCCAGATCGGAAGGTTGCGTGTTCAAATC *F ACGTCGGGGTCA *F >gb|AE002620|AE002620.trna4-TyrGTA (216345-216238) Tyr (GTA) 108 bp Sc: 75.42 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGACGGTTTCATATTGAATGCAGAA *F TACAGAGCAGAGATCCATAGGTCGCTGGTTCAAGTCCGGCTCGAAGGA *F >gb|AE002620|AE002620.trna1-TyrGTA (216526-216621) Tyr (GTA) 96 bp Sc: 67.06 *F CCTTCGAATAGCTCAGTTGGTAGAGCGGTGGACTGTAGATGGTAAATCGCAATGGCAGAG *F ATCCATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002638|AE002638.trna13-TyrGTA (2480226-2480133) Tyr (GTA) 94 bp Sc: *F 77.84 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGATTGGGATTACGAATGTAGACAT *F CCATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002638|AE002638.trna3-TyrGTA (2482775-2482895) Tyr (GTA) 121 bp Sc: *F 73.79 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGAAGTTTTCAGATCTGATCACACT *F TTCCAGGTGATCGAATCCAGCAGGCATCCATAGGTCGCTGGTTCAAATCCGGCTCGAAGG *F A *F >gb|AE002708|AE002708.trna61-TyrGTA (930016-929924) Tyr (GTA) 93 bp Sc: 78.51 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGTTGGCAAACAAGCAATAGAAATC *F CATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002708|AE002708.trna60-TyrGTA (930384-930292) Tyr (GTA) 93 bp Sc: 79.28 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGTTGGAAAACAAGCAATAGAAATC *F CATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002708|AE002708.trna59-TyrGTA (955798-955706) Tyr (GTA) 93 bp Sc: 79.14 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGTTGGAAAACATGCAATAGAAATC *F CATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002708|AE002708.trna58-TyrGTA (956295-956202) Tyr (GTA) 94 bp Sc: 79.90 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGTTGGAAAATTATGCAATAGAAAT *F CCATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002708|AE002708.trna57-TyrGTA (956764-956671) Tyr (GTA) 94 bp Sc: 79.41 *F CCTTCGATAGCTCAGTTGGTAGAGCGGTGGACTGTAGTTGGAAAAACATGCAATAGAAAT *F CCATAGGTCGCTGGTTCAAATCCGGCTCGAAGGA *F >gb|AE002787|AE002787.trna36-Undet? (8380310-8380239) Undet (?) 72 bp Sc: *F 37.58 *F GCCGTGATCGTCTAGTGGTTAGGACCCCACGTTGTGGGTTACCACACCCAGGTTCGAATC *F CTGGTCACGGCA *F >gb|AE002602|AE002602.trna22-ValAAC (6831198-6831126) Val (AAC) 73 bp Sc: *F 79.44 *F GTTTCCGTGGTGTAGCGGTTATCACATCTGCCTAACACGCAGAAGGCCCCCGGTTCGATC *F CCGGGCGGAAACA *F >gb|AE002602|AE002602.trna3-ValAAC (6830528-6830600) Val (AAC) 73 bp Sc: 79.44 *F GTTTCCGTGGTGTAGCGGTTATCACATCTGCCTAACACGCAGAAGGCCCCCGGTTCGATC *F CCGGGCGGAAACA *F >gb|AE002787|AE002787.trna51-ValAAC (1339333-1339261) Val (AAC) 73 bp Sc: *F 79.44 *F GTTTCCGTGGTGTAGCGGTTATCACATCTGCCTAACACGCAGAAGGCCCCCGGTTCGATC *F CCGGGCGGAAACA *F >gb|AE002787|AE002787.trna52-ValAAC (1338907-1338835) Val (AAC) 73 bp Sc: *F 79.44 *F GTTTCCGTGGTGTAGCGGTTATCACATCTGCCTAACACGCAGAAGGCCCCCGGTTCGATC *F CCGGGCGGAAACA *F >gb|AE002708|AE002708.trna47-ValAAC (9862633-9862561) Val (AAC) 73 bp Sc: *F 80.36 *F GTTTCCGTGGTGTAGTGGTTATCACATCCGCCTAACACGCGGAAGGCCCCCGGTTCAATC *F CCGGGCGGAAACA *F >gb|AE002708|AE002708.trna7-ValAAC (8555025-8555097) Val (AAC) 73 bp Sc: 80.36 *F GTTTCCGTGGTGTAGTGGTTATCACATCCGCCTAACACGCGGAAGGCCCCCGGTTCAATC *F CCGGGCGGAAACA *F >gb|AE002699|AE002699.trna1-ValCAC (220703-220775) Val (CAC) 73 bp Sc: 81.63 *F GTTTCCGTAGTGTAGCGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGGGAACA *F >gb|AE002699|AE002699.trna12-ValCAC (245381-245309) Val (CAC) 73 bp Sc: 81.63 *F GTTTCCGTAGTGTAGCGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGGGAACA *F >gb|AE002699|AE002699.trna13-ValCAC (244618-244546) Val (CAC) 73 bp Sc: 81.63 *F GTTTCCGTAGTGTAGCGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGGGAACA *F >gb|AE002699|AE002699.trna2-ValCAC (224546-224618) Val (CAC) 73 bp Sc: 81.63 *F GTTTCCGTAGTGTAGCGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGGGAACA *F >gb|AE002708|AE002708.trna20-ValCAC (12017657-12017729) Val (CAC) 73 bp Sc: *F 81.63 *F GTTTCCGTAGTGTAGCGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGGGAACA *F >gb|AE002708|AE002708.trna41-ValCAC (11899837-11899765) Val (CAC) 73 bp Sc: *F 81.63 *F GTTTCCGTAGTGTAGCGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGGGAACA *F >gb|AE002708|AE002708.trna9-ValCAC (9851236-9851308) Val (CAC) 73 bp Sc: 83.37 *F GTTTTCGTAGTGTAGTGGTTATCACGTGTGCTTCACACGCACAAGGTCCCCGGTTCGAAC *F CCGGGCGAAAACA *F >gb|AE002602|AE002602.trna12-ValTAC (14869231-14869303) Val (TAC) 73 bp Sc: *F 79.18 *F GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTTACACGCAGAAGGTCTCCGGTTCGATC *F CCGGATGGAACCA *F >gb|AE002602|AE002602.trna14-ValTAC (14869622-14869550) Val (TAC) 73 bp Sc: *F 79.18 *F GGTTCCATAGTGTAGCGGTTATCACGTCTGCTTTACACGCAGAAGGTCTCCGGTTCGATC *F CCGGATGGAACCA *F \------------------------------------------------------------------------------ *F -- # *U FBrf0145625 *a Bloomington Drosophila Stock Center *b ?. *t 2002.1.16 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Jan 16 16:06:26 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GawB}c179 insertion *F The P{GawB}c179 insertion (FBti0004029) maps to the second chromosome based *F on the segregation of the miniwhite marker from CyO in the cross w1 *F females x w1118; P{w+mW.hs=GawB}C179/CyO; \+/TM3, Sb1 Ser1 *F males. The insertion is homozygous viable and fertile. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145626 *a Matthews *b K. *t 2002.1.16 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Wed Jan 16 17:21:34 2002 *F Subject: Dp(4;3)f *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Kathy Matthews, Bloomington Drosophila Stock Center *F Subject: Dp(4;3)f *F Information communicated: *F The aberration described as Tp(4;3)f is really Dp(4;3)f. The aberration was *F originally described as a T(3;4) and was apparently renamed as a *F transposition based on the cytology reported by Lewis (1956, DIS 30:130): *F 'Insertion of at least seven bands of chromosome four (bands not identified) *F into 3L, probably just after 65D1-2.' Lewis did not, however, describe a *F reciprocal 4th chromosome deficiency. The two extant stocks (Bloomington *F numbers 894 & 895) came to us as Tp(4;3)f with a 3rd chromosome balancer *F specified but no 4th chromosome homologue specified. I tested stock number *F 895 for the presence of the duplication and deficiency segregants and found *F only the duplication. *F The incorrect genotypic information has led to some confusion so I'll *F summarize the complementation data for this duplication below. The *F genotypes tested by Rui Sousa-Neves (see FBrf0112252 & FBrf0125105) and *F me were as follows: *F Dp(4;3)f/+; svspa-1 *F The duplication complements svspa-1 (this genotype has a wild- *F type eye); Sousa-Neves (FBrf0112252) & Matthews (this pc) *F Dp(4;3)f/+; T(1;4)scH/pho1 *F The duplication fails to complement the lethality of T(1;4)scH/ *F pho1 (this genotype is lethal); Sousa-Neves (FBrf0125105; *F the genotype tested was clarified in discussion with me) *F Dp(4;3)f/+; In(4)ciD, ciD panciD/pan3 *F The duplication fails to complement panciD/pan3 (this *F genotype is lethal); Sousa-Neves (FBrf0112252) *F Thus, Dp(4;3)f is sv+, pho- and pan-. It is not known whether it is *F ci+ or ci-. Tp(4;3)f and Df(4;3)f almost certainly do not exist. # *U FBrf0145627 *a Cook *b K. *t 2002.1.18 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jan 18 16:42:53 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Revised cytology of Df(3R)23D1 *F I reanalyzed the polytene cytology of Df(3R)23D1 and found the breakpoints *F to be 94A3-4;94D1-4. The deletion removes most of 94D1-4, leaving only a *F thin portion from the distal edge of the quadruplet. The 94A3,4 doublet is *F nearly intact. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145628 *a Deal-Herr *b M. *c K. *d Bogart *e K. *f Cook *t 2002.1.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 22 15:36:15 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2R)BSC11 *F Isolation and characterization of Df(2R)BSC11 *F Megan Deal-Herr, Kevin Bogart and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2R)BSC11 was isolated as a P transposase-induced male recombination *F event involving P{lacW}l(2)s3475s3475 and P{PZ}chn02064. The deletion *F was isolated as a cn+-bw+ recombinant chromosome from the cross cn1 *F bw1 females x cn1 P{PZ}chn02064/P{lacW}l(2)s3475s3475 bw1 sp1; *F TMS, Sb1 P{ry+t7.2=Delta2-3}99B/+ males. Polytene chromosome squashes *F showed the breakpoints 50E6-F1;51E2-4. Df(2R)BSC11 failed to complement *F phyl2245, Rpn6k00103, and Asx1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145629 *a Cadigan *b K. *t 2002.1.24 *T personal communication to FlyBase *u *F From cadigan@umich.edu Thu Jan 24 22:42:35 2002 *F To: Rachel Drysdale *F Subject: Re: gammy legs *F Dear Rachel, *F Please consider the attachment a personal communication for now. We have *F submitted a manuscript and well let you know when it is in press. Please *F let me know if there is any other information you require. *F Regards, *F Ken *F ===================================================================== *F Previously known as BEST:LD21971 *F Gene: gam *F Symbol: gam *F Full name: gammy legs *F D. melanogaster gene gammy legs, abbreviated as gam, is reported here. *F It encodes a PHD- finger protein, which is localized to the nucleus. *F It is ubiquitously expressed at low levels in the embryo and imaginal *F discs. Similar sequences have been identified in Homo sapiens. It has *F been sequenced and its amino acid sequence is also avaliable. It has *F been mapped cytologically to 100C7--D. It interacts genetically with *F arm, dsh and axin, and acts genetically as a positive regulator of *F Wingless signaling. Null mutations have been isolated that affect the *F epidermis, denticle belts, antenna, legs and wings and are recessive *F viable. There is one recorded mutant allele, but it is not available *F from the public stock centers. gammy legs is discussed in 5 references, *F dated between 1999 and 2001. These include at least 2 molecular *F studies. *F GENE PRODUCT *F Biological process: Wingless signaling pathway *F Rep. Protein sequence: 815 aa, 2000 (full cDNA sequence submitted to genbank) *F Protein domains: PHD-finger domain *F GENOMIC ORGANISATION *F Genomic sequence analysis: full cDNA sequence submitted to Genbank *F Cytogenic map: 100C6-7 *F Rep DNA sequence: Genebank accession number AY075095 *F ===================================================================== *F \--On Tuesday, January 22, 2002 8:17 PM \+0000 Rachel Drysdale *F wrote: *F > Dear Ken, *F > *F >> I would like to update the information on the gammy legs gene *F > *F > brilliant! If the information about gammy legs is soon to be published *F > then just let us know where and we will look out for it. However if *F > you want to tell us things that will not be due for publication soon *F > then just send us an email (to flybase-updates@morgan.harvard.edu is *F > fine or to me direct) and we will curate it as a personal communication *F > to FlyBase from you. Don't worry about the format or content \- we can *F > go back and forth if necessary until we are all happy with the text. *F > *F > Best regards, *F > *F > Rachel. *F > for the FlyBase consortium. # *U FBrf0145630 *a Deal *b J. *c K. *d Cook *t 2002.1.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 22 17:36:28 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC12 *F Isolation and characterization of Df(3L)BSC12 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center *F Df(3L)BSC12 was isolated as a P transposase-induced male *F recombination *F event involving P{PZ}l(3)0147001470 and P{EP}capsEP3557. The deletion *F was isolated as a rhove-1-e1 recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{PZ}l(3)0147001470/P{EP}capsEP3557 e1 males. The miniwhite markers *F from P{lacW} and P{EP} were deleted or disrupted based on the w- eye *F color of flies carrying the deletion chromosome in the presence of w1. *F Df(3L)BSC12 failed to complement P{lacW}trnS064117 and In(3LR)C190. *F In polytene squashes of Df(3L)BSC12 heterozygotes, the 70A1,2 *F doublet *F appeared attenuated, but not absent. The 70A4,5 doublet and 69F5 were *F present, but the 69F6, 69F7 and 70A3 bands were not visible in our *F preparations. Observations detailed below argue that the distal side of *F 70A1,2 was deleted, so we have denoted the breakpoints of Df(3L)BSC12 as *F 69F6-70A1;70A1-2. *F To reconcile the noncomplementation of Df(3L)BSC12 and the 3L *F breakpoint *F of In(3LR)C190, we reexamined the polytene cytology of In(3LR)C190. We *F found that the 3L breakpoint of In(3LR)C190 lies within the 70A1,2 doublet, *F splitting off a distal portion of the doublet (likely just the distal edge *F of 70A1) as a thin, single band. Our revised cytology of In(3LR)C190 is *F 70A1-2;89D3-6. *F Our screens for Df(3L)BSC10 (69D4-5;69F5-7) and Df(3L)BSC12 *F (69F6-70A1;70A1-2) were designed to isolate deletions that flank M(3)69E as *F closely as possible. Two closely-linked ribosomal protein genes, RpS12 and *F RpS4, lie between the predicted endpoints of the two deletions (Adams, M.D. *F et al. 2000, Science 287: 2185-2195). Our work does not indicate whether *F M(3)69E corresponds to RpS12 or RpS4, or whether the genetically-defined *F M(3)69E locus is a composite of both ribosomal protein genes. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F __________________________________________________________ # *U FBrf0145631 *a Manning *b G. *t 2002.2.8 *T personal communication to FlyBase *u *F From gerard-manning@sugen.com Fri Feb 08 01:22:24 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: Change CG4803 to TakL2? *F Hi, *F Some time ago, I submitted a new predicted gene, TakL1, to *F Flybase, named after its similarity to the Tak1 immune kinase *F (http://flybase.bio.indiana.edu/.bin/fbpcq.html?FBrf0139873). CG4803 is a *F second Tak1 homolog in fly; I would like to suggest that this be renamed *F TakL2; we are writing up a paper which mentions all three, so a common *F nomenclature would be helpful. *F Many thanks, *F \-Gerard. # *U FBrf0145632 *a Manning *b G. *t 2002.2.8 *T personal communication to FlyBase *u *F From gerard-manning@sugen.com Fri Feb 08 01:27:21 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: Change CG4803 to TakL2? *F Hi, *F The gene predictions CG3915 and CG12463 appear to be part of the same *F gene, by homology and co-mapping. The combined gene has clear homolog to *F doughnut (dnt) and derailed (drl); I would like to suggest that it be *F renamed doughnut-like (dnl), whose acronym is suitably related to both drl *F and dnt. We have a sequence which will be deposited with Swiss-Prot/TrEMBL, *F but would appreciate an early decision on the renaming, for a manuscript *F which is just about to be submitted. *F Many thanks, *F \-Gerard. # *U FBrf0145633 *a Hong *b C. *c C. *d Hashimoto *t 2002.2.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 11 14:33:14 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Deletions from Hong and Hashimoto *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Charles Hong and Carl Hashimoto of Yale University *F (1/02). Chromosomal deletions isolated in the screen described in Hong and *F Hashimoto, 1995 (Cell 82: 785--794; FBrf0083198) have the following *F cytological breakpoints. *F Df(3L)CH12 65A7-11;65C1-3 *F Df(3L)CH13 64F-65A;65C4 *F Df(3L)CH20 64D1-2;65C3 *F Df(3L)CH38 64E7;65E4 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145634 *a Jacobs *b R. *t 2002.2.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 11 17:49:57 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-sli.B}3 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Roger Jacobs of McMaster University (1/02). *F P{UAS-sli.B}3 is a homozygous viable and fertile, third chromosome insertion. *F The construction and insertion of P{UAS-sli.B} was described in Battye et *F al. J. Neurosci. 21: 4290-4298, 2001 (FBrf0136993). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145635 *a Montell *b D. *t 2002.2.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Feb 12 15:25:06 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GAL4-slbo.2.6}1206 insertion *F The following information accompanied a stock from Denise Montell, Johns *F Hopkins Medical School (1/02). *F P{GAL4-slbo.2.6}1206 is a second chromosome insertion. The construction and *F transformation of P{GAL4-slbo.2.6} were described in Rorth et al., 1998, *F Development 125: 1049-1057 (FBrf0101995). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145636 *a Roote *b J. *t 2002.2.6 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Wed Feb 06 13:43:02 2002 *F To: rd120@mole.bio.cam.ac.uk *F Subject: Df kit *F rachel, *F We have made the following deletions: *F deletion LH insert scaffold(A) RH insert scaffold(B) cyt (inferred) kb(B-A) *F Df(2R)ED1 P{RS3r}CB-0140-3 11989287 P{RS5r}5-HA-1118 12059882 53F1; 53F11 *F 70595 *F Df(3R)ED2 P{RS3r}CB-0160-3 14158440 P{RS5r}5-HA-1087 14856038 91A2;91F2 *F 697598 *F Df(2L)ED3 P{RS5r}5-HA-1150 14377917 P{RS3r}CB-0183-3 15218480 35A;35D 840563 *F Genetic evidence: *F ED1 lethal with Df(2R)P803-&Dgr;15 and In(2LR)DTD99. Note also that *F DTD99 is lethal over P803-delta15. *F ED2 lethal with (TBA) *F ED3 is el+, noc-,osp-, nht-, esg+, enhances Scutoid phenotype. *F verification by sequence is TBA. I may have mentioned that there is *F an inherent problem with sequencing these Dfs \- they have two 3' P *F ends (i.e. there is 3' P at both ends of the reconstituted element). *F J # *U FBrf0145637 *a Roote *b J. *t 2002.2.18 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Mon Feb 18 17:59:03 2002 *F To: rd120@mole.bio.cam.ac.uk *F Subject: ospPW *F and another PC to flybase, correcting an earlier PC, FBrf0099485. *F ospPW is derived from k08808 and is mutant for osp and retains a *F Pw+. It was isolated as an osp allele in a screen for local hops. *F OK so far: but it's actually a deletion of osp to 35Bc (Adh status unknown). *F These data and the screen design suggest the deletion may have *F occurred as a result of a male recombination event. I'm tempted to *F call it k08808-mr1. *F Oh dear. *F J # *U FBrf0145638 *a McGinnis *b W. *c T. *d Kaufman *t 2002.2.18 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Mon Feb 18 17:34:45 2002 *F Subject: Dfd1 is homozygous viable *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Dfd1 is homozygous viable *F Dated: 18 Feb 2002 *F Background: Conflict between a Bloomington stock genotype and information *F in FlyBase led to the following information being passed along to FlyBase. *F Information communicated: Although Dfd1 has been stated to be *F recessive lethal, both Bill McGinnis (U.C. San Diego) and Thom Kaufman *F (Indiana U.) confirm that homozygous viable Dfd1-bearing chromosomes *F have been recovered. # *U FBrf0145639 *a Bloomington Drosophila Stock Center *b ?. *t 2002.2.21 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Feb 21 20:25:36 2002 *F Subject: new gene symbols *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: gene symbols *F Dated: 21 Feb 2002 *F We need gene symbols for 6-phosphofructo-2-kinase and katanin-80 and will use *F Pfrx and kat80, respectively. # *U FBrf0145640 *a Bloomington Drosophila Stock Center *b ?. *t 2002.2.21 *T personal communication to FlyBase *u *F From matthewk@mail-relay.indiana.edu Thu Feb 21 20:30:23 2002 *F Subject: another synonym *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: misguided5 *F Dated: 21 Feb 2002 *F misguided5 is a synonym for Lamsz18. # *U FBrf0145641 *a Green *b M.M. *t 2002.2.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Feb 24 21:07:40 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Dp(1;1)MMG *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Melvin M. Green, University of California at Davis (2/02). *F Dp(1;1)MMG ('Mel's Metacentrigenic') was generated from recombination *F between C(1)RM and T(1;4)BS. It is a reconstruction of Muller's *F Dp(1;1)BSRMG (FBab0003041) and has the same structure. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145642 *a Sung *b C. *c S. *d Robinow *t 2002.2.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Feb 24 21:38:30 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-EcR.B2}3a, P{UAS-EcR.B1}3b and P{UAS-EcR.A}3a insertions *F The following information was received from Carl Sung and Steven Robinow, *F University of Hawaii (2/02). *F P{UAS-EcR.B2}3a, P{UAS-EcR.B1}3b and P{UAS-EcR.A}3a are all homozygous *F viable and fertile third chromosome insertions. *F The constructs and their transformation was described in Lee et al. 2000, *F 'Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for *F mushroom body neuronal remodeling in Drosophila', Neuron 28:807-818 *F (FBrf0132434). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145643 *a Deal *b J. *c K. *d Cook *t 2002.2.25 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 25 00:43:15 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC13 *F Isolation and characterization of Df(3L)BSC13 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(3L)BSC13 was isolated as a P transposase-induced male recombination *F event involving P{lacW}Nmtj1C7 and P{EP}EP3212. The deletion was isolated *F as a rhove-1-e1 recombinant chromosome from the cross rhove-1 pp *F e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{lacW}Nmtj1C7/P{EP}EP3212 e1 males. Polytene chromosome squashes *F showed the breakpoints 66B12-C1;66D2-4. Df(3L)BSC13 failed to complement *F the recessive lethality of T(2;3)E(da) and P{PZ}l(3)0392803928. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145644 *a Deal *b J. *c K. *d Cook *t 2002.2.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 25 01:44:44 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC15 *F Isolation and characterization of Df(3L)BSC15 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC15 was isolated as an unexpected deletion event in the same screen *F that produced Df(3L)BSC10. Df(3L)BSC15 was recovered as a rhove-1-e1 *F recombinant chromosome from the cross rhove-1 pp e1 females x CyO, *F H{PDelta2-3}HoP2.1/+; rhove-1 P{lacW}mirrcre2/P{lacW}RpS12s2783 e1 *F males. Df(3L)BSC15 does not delete the chromosomal region in 69DF between *F the P insertions; instead, it has the breakpoints 70A6-7;70B6-7. It failed *F to complement the recessive lethality of l(3)0054300543 and *F l(3)L5212L5212, but complemented Df(3L)Ly, Lysens-E58, T(Y;3)A31 and *F T(Y;3)R83 for lethality and v(3)0369903699 for wing vein defects. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145645 *a Deal *b J. *c K. *d Cook *t 2002.2.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 25 01:45:04 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC14 *F Isolation and characterization of Df(3L)BSC14 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC14 was isolated as a P transposase-induced male recombination *F event involving P{PZ}Dhh1rL562 and P{lacW}l(3)01239j9B4. The deletion *F was isolated as a rhove-1-e1 recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{PZ}Dhh1rL562/P{lacW}l(3)01239j9B4 e1 males. Polytene chromosome *F squashes showed the breakpoints 67E3-7;68A2-6. Df(2L)BSC14 failed to *F complement l(3)0181401814. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145646 *a Cook *b K. *t 2002.2.25 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Feb 25 02:24:22 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Revised Df(3L)lxd6 cytology *F I reexamined polytene chromosome squashes of Df(3L)lxd6 and found the *F cytological breakpoints to be 67E5-7;68B2-4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145647 *a Freeman *b M. *t 2002.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:51:41 2002 *F To: freeman@uoneuro.uoregon.edu *F Subject: Helping FlyBase: CSH-84 *F Dear Marc, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The genomics of gcm-dependent glial cell development: identification of gcm *F target genes by computational and DNA microarray approaches.' *F You mention a gene that is new to FlyBase, draper. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From freeman@uoneuro.uoregon.edu Tue Mar 05 16:51:35 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-84 *F Hi Rachel, *F Here's some info. draper (drpr) is CG2086, aka BcDNA:gh03529--we thought *F we'd call it drpr to make it a little more interesting, it's looks like its *F involved in glial morphogenesis in the embryonic CNS. Our molecular *F analysis of some full length cDNAs tells us that another gene that is *F annotated inside a drpr intron, CG18172, is actually the same gene. We'll *F update the locus as soon as we're done with the molecular analysis, but you *F may want to list both of the above CG#s with drpr. *F Let me know if I can provide any other information. best, Marc *F Marc R. Freeman *F Institute of Neuroscience/HHMI *F 1254 University of Oregon *F Eugene, OR 97403-1254 *F (541)346-3041 # *U FBrf0145648 *a Featherstone *b D. *t 2002.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:50:41 2002 *F To: featherstone@biology.utah.edu *F Subject: Helping FlyBase: CSH-26 *F Dear David, *F We are currently curating the abstracts for the 2001 CSH Neurobiology of *F Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'bad reception (brec) is essential for glutamate receptor field *F formation.' *F You mention a gene that is new to FlyBase, brec. Now that some time *F has passed I wonder do you know which of the Genome Project CG *F annotations your gene corresponds to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F Even if you don't know which CG brec is then perhaps you could tell me *F its map location, as this is valuable information in its own right. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From featherstone@biology.utah.edu Tue Mar 05 17:01:39 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-26 *F Hi Rachel, *F I do not yet know what CG gene 'bad reception' corresponds to, *F although hopefully this will be changing within the next few weeks, *F since we now appear to have some P-inserts. I'll certainly let you *F know the CG gene ASAP once we know. As for map position, the best I *F can tell you is 92E, which as close as we've been able to map it. *F Thanks for the continuing great work on Flybase. *F Dave *F Dave Featherstone *F University of Utah *F Dept. of Biology *F 257 South 1400 East *F Salt Lake City, UT 84112 # *U FBrf0145649 *a Kuchinke *b U. *c U. *d Thomas *t 2002.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:53:03 2002 *F To: kuchinke@ifn-magdeburg.de *F Subject: Helping FlyBase: CSH-222 *F Dear Ute, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Fly homologues of PROSAP and MAGI are putative scaffolding molecules *F at larval neuromuscular junctions.' *F You mention two genes that are new to FlyBase, Prosap and Magi. Do you *F know which of the Genome Project CG annotations your genes correspond *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If either of the genes do not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kuchinke@ifn-magdeburg.de Tue Mar 05 18:24:35 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-222 *F Dear Rachel, *F the Drosophila homolog of the synaptic scaffolding protein ProSAP/Shank *F corresponds to CG13354 and CG8122. The (putative) Drosophila homolog of *F MAGI also covers two neighbouring CGs, i.e. CG15656 and CG4117. *F We hope this will be helpful for you, *F Many thanks for all your work \! *F Greetings *F Ute Kuchinke and Uli Thomas *F Dr. Ute Kuchinke *F Leibniz Institute for Neurobiology *F Dept. of Neurochemistry and Molecular Biology *F Brenneckestr. 6, D-39118 Magdeburg, Germany; *F Phone: \+49/391/6263-202 *F Fax: \+49/391/6263-229 *F E-mail: kuchinke@ifn-magdeburg.de *F Internet: http://www.ifn-magdeburg.de # *U FBrf0145650 *a Tschaepe *b J.A. *t 2002.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:57:32 2002 *F To: jakob.tschaepe@biozentrum.uni-wuerzburg.de *F Subject: Helping FlyBase: CSH-201 *F Dear Jakob-Andreas, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The drosophila mutant lochrig (loe) \- neurodegeneration and *F cholesterol metabolism.' *F You mention a gene that is new to FlyBase, loe. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jakob.tschaepe@biozentrum.uni-wuerzburg.de Tue Mar 05 18:27:01 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-201 *F Dear Rachel, *F yes, I know which gene prediction corresponds to the loe mutant: It *F is the gamma subunit of the AMP activated protein kinase complex *F in Drosophila (as mentioned in the abstract...) In the databases *F this is registered as SNF4Agamma or AMPK gamma. For this *F SNF4 protein a predicted sequence already exists in *F FlyBase, based on a sequence published by Yoshida et al 1999. *F The gene annotation has got the number CG17299. *F ... *F with best regards, *F Jakob *F Jakob-Andreas Tschaepe *F Dept. of Genetics and Neurobiology *F AG Pflugfelder *F University of Wuerzburg *F Biozentrum Am Hubland *F 97074 Wuerzburg *F Germany *F \+49 931-8884457 # *U FBrf0145651 *a Odden *b J. *t 2002.3.5 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:54:27 2002 *F To: jpodden@darkwing.uoregon.edu *F Subject: Helping FlyBase: CSH-157 *F Dear Joanne, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A conserved mechanism for motoneuron development? The role of DHB9 in *F specifying motoneuron fate and ventral axon projections.' *F You mention a gene that is new to FlyBase, HB9. Do you know which of *F the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. It seems possible that HB9 corresponds to *F CG8254 (FBgn0035828) or slou (FBgn0002941) but you would know for *F sure. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jpodden@darkwing.uoregon.edu Tue Mar 05 19:08:29 2002 *F To: Rachel Drysdale (Genetics) *F Subject: RE: Helping FlyBase: CSH-157 *F Hi Rachel, *F Yes DHB9 corresponds to annotation CG8254 and the gene exex (though it hasn't *F been published, it is in flybase and there was an abstract at this year's CSH *F Dros Neuro meeting). Let me know if you have any other questions! *F Thanks, *F Joanne Odden # *U FBrf0145652 *a Deal *b J. *c K. *d Cook *t 2002.3.5 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Mar 01 19:03:31 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC17 *F Isolation and characterization of Df(2L)BSC17 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC17 was isolated as a P transposase-induced male recombination *F event involving P{PZ}pelo1 and P{EP}EP460. The deletion was isolated as a *F dp+-cn+ recombinant from the cross dpov1 cn1 bw1 females x *F P{PZ}pelo1 cn1/dpov1 P{EP}EP460; TMS, P{Delta2-3}99B/+ males. *F Polytene chromosome squashes showed the breakpoints 30C3-5;30F1. *F Df(3L)BSC17 failed to complement the lethality of sopk01215 and the wing *F vein defects of zf30Ck02506. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145653 *a Santschi *b L.A. *t 2002.3.6 *T personal communication to FlyBase *u *F From santschi@salk.edu Tue Mar 05 22:11:43 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-228 and 235 *F Dear Rachel, *F There are two CGs corresponding to Drl-2: *F CG12463 & CG3915. *F Best regards, *F Linda *F \-- *F Linda A. Santschi, Ph.D. *F Molecular Neurobiology Laboratory *F The Salk Institute for Biological Studies *F 10010 N Torrey Pines Road *F La Jolla, CA 92037 *F Ph. (858) 453-4100 ext. 1360 *F FAX. (858) 455-6138 *F email santschi@salk.edu # *U FBrf0145654 *a Kraut *b R. *t 2002.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:52:42 2002 *F To: rkraut@cco.caltech.edu *F Subject: Helping FlyBase: CSH-221 *F Dear Rachel, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Novel findings from an overexpression screen: vesicle trafficking in *F pathfinding and robo in sense organ formation.' *F You mention two genes that are new to FlyBase, Beached and *F Gartenzwerg. Do you know which of the Genome Project CG annotations *F your genes correspond to? All the CGs have corresponding gene records *F in FlyBase already and we don't like to make duplicate records for what *F is actually the same gene unless we can't avoid it. If either of them *F do not correspond to a CG then perhaps you could tell me its map *F location, as this is valuable information for the genome annotation *F project. If you have short symbols in mind for Beached and Gartenzwerg *F then if you let me know them I can put them into FlyBase along with *F their full names. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From rkraut@cco.caltech.edu Wed Mar 06 06:16:48 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: CSH-221 *F Dear Rachel, *F Beached corresponds to CG9011 and Gartenzwerg to CG8487. They could be *F abbreviated as bch and garz, in case these aren't *F already taken. *F Thank you, *F Rachel *F From rd120@gen.cam.ac.uk Wed Mar 06 14:05:30 2002 *F To: rkraut@cco.caltech.edu *F Subject: Re: Helping FlyBase: CSH-221 *F Hi Rachel, *F Thank you for your quick response. Couple of things \- I gather from *F your mail that you prefer the lower case versions beached and *F gartenzwerg (the symbols and names should have the same *F capitalization). bch (as a symbol) will not work as bch is already the *F symbol for the branch gene (FBgn0000167). How about bchd? Little bit *F longer but still short enough for a proper symbol. garz is fine for *F gartenzwerg. *F All the best, *F Rachel. *F From rkraut@cco.caltech.edu Thu Mar 07 05:03:12 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: CSH-221 *F Dear Rachel, *F Sure, those are fine. I guess they should be written lower case, since *F these genes don't have any dominant phenotype that I know of. At least *F that's not how they were identified. *F Thanks for the work, *F Rachel *F Note: CG9011 = Beach1 therefore bchd == Beach1 (FBgn0043362). # *U FBrf0145655 *a Botella *b J.A. *t 2002.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:49:17 2002 *F To: jabotella@hotmail.com *F Subject: Helping FlyBase: CSH-165 *F Dear Jose, *F We are currently curating the abstracts for the 2001 CSH Neurobiology of *F Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of novel genes and mechanisms of neurodegeneration in *F Drosophila.' *F You mention a gene that is new to FlyBase, sniffer. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jabotella@hotmail.com Wed Mar 06 10:03:26 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: CSH-165 *F Dear Rachel, *F Sniffer corresponds to CG10964, the abbreviated name we use for sniffer *F is 'sni'. *F Best wishes, *F jose # *U FBrf0145656 *a Faivre-Sarrailh *b C. *t 2002.3.6 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:50:21 2002 *F To: sarrailh@lgpd.univ-mrs.fr *F Subject: Helping FlyBase: CSH-209 *F Dear Catherine, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila F3/ Contactin is required in axonal insulation.' *F You mention a gene that is new to FlyBase, Cont. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From sarrailh@ibdm.univ-mrs.fr Wed Mar 06 13:14:32 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-209 *F Dear Rachel Drysdale, *F The Drosophila gene that we have named Dcontactin correspond to CG1084. *F According to flybase annotations it is homologous to human TAG-1 and chick *F axonin-1, but according to the blasts that we have made, it is also *F homologous to human and mouse contactin. We have sequenced the cDNA and it *F encodes a protein of 1390 aa instead of the predicted 1336 aa *F Please tell me if you need other informations about this gene. *F Sincerely yours, *F Catherine *F Catherine Faivre-Sarrailh *F Neurobiologie des Interactions Cellulaires et Neuropathologie *F CNRS FRE 2533 *F IFR Jean Roche *F Present address: *F UMR 6545-IBDM *F Faculté des Sciences de Luminy *F 13288 Marseille cedex 9 *F Lab tel : 33 (0)4 91 26 97 28 *F Office: 33 (0)4 91 26 93 47 *F Fax: 33 (4) 91 26 97 26 # *U FBrf0145657 *a Cook *b K. *t 2002.3.7 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Mar 01 18:11:41 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Phenotypes and complementation of zf30C mutations *F Phenotypes and complementation of zf30C mutations *F Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F This report explores the phenotypes associated with P element insertions at *F the zf30C gene. When I began my analysis, the Berkeley Drosophila Genome *F Project (Adams et al, 2000; Spradling et al., 1999) had documented the *F following facts. (1) The P{lacW}zf30Ck02506, P{lacW}zf30Ck02510, *F P{EP}zf30CEP518 and P{EP}zf30CEP2228 insertions all map to the zf30C *F gene. (2) The P{lacW}zf30Ck02506 and P{lacW}zf30Ck02510 are *F nonindependent insertions and the chromosomes carrying the insertions *F failed to complement for lethality. (3) The chromosomes carrying *F P{lacW}zf30Ck02506 and P{lacW}zf30Ck02510 complemented Df(2L)30A-C and *F Df(2L)s1402 for lethality, but failed to complement Df(2L)N22-5. (4) The *F chromosome carrying P{lacW}zf30Ck02506 complemented Df(2L)N22-3 for *F lethality. *F Df(2L)30A-C deletes the pelo (Castrillon et al., 1993) and und (Cutforth *F and Gaul, 1999) genes flanking zf30C, but Df(2L)30A-C complements *F P{lacW}zf30Ck02506 and P{lacW}zf30Ck02510 for lethality (Adams et al, *F 2000; Spradling et al., 1999). This, and the fact that P{EP}zf30CEP518 *F and P{EP}zf30CEP2228 are homozygous viable, led me to examine crosses *F between zf30C alleles and deletions of 30C. I found that the zf30C *F mutations cause wing and female fertility phenotypes, but not lethality. My *F observations are detailed below. *F 1. The severity of the zf30C alleles could be ranked *F zf30Ck02506EP518EP2228 in terms of wing phenotype. *F zf30Ck02506 in combination with Df(2L)30A-C, Df(2L)gamma7, Df(2L)N22-3 or *F Df(2L)s1402 gave incompletely penetrant shortening of the L5 wing vein and *F posterior crossvein. L5 vein shortening was seen in a minority of flies and *F PCV shortening was uncommon. zf30CEP518 in combination with Df(2L)30A-C, *F Df(2L)gamma7, Df(2L)N22-3, Df(2L)s1402, Df(2L)N22-5 or Df(2L)N22-14 had *F slightly more penetrant L5 and PCV shortening and occasional shortening of *F L2. In addition, I observed an incompletely penetrant outspread wing *F phenotype. zf30CEP2228 in combination with Df(2L)30A-C, Df(2L)gamma7, *F Df(2L)N22-3, Df(2L)s1402, Df(2L)N22-5 or Df(2L)N22-14 gave highly penetrant *F L5, PCV and L2 shortening. Wings were usually outspread and, in the *F severest cases, dragged on the ground. I saw some nicking of the wing margins. *F 2. zf30C alleles showed the same allelic strength *F (zf30Ck02506EP518EP2228) regarding female fertility. *F Females carrying zf30Ck02506 in combination with Df(2L)30A-C, *F Df(2L)gamma7, Df(2L)N22-3 or Df(2L)s1402 were all fertile. Females carrying *F zf30CEP518 in combination with Df(2L)30A-C, Df(2L)gamma7, Df(2L)N22-3, *F Df(2L)s1402, Df(2L)N22-5 or Df(2L)N22-14 were either sterile or weakly *F fertile. Females carrying zf30CEP2228 in combination with Df(2L)30A-C, *F Df(2L)gamma7, Df(2L)N22-3, Df(2L)s1402, Df(2L)N22-5 or Df(2L)N22-14 were *F invariably sterile. Females with any of the sterile genotypes laid a few *F nonhatching eggs and had ovaries of normal size with no overt morphological *F defects. *F 3. No male sterility was seen with any zf30C genotypes. *F 4. The phenotypes of zf30CEP518 and zf30CEP2228 in combination with *F zf30Ck02506 were less severe than phenotypes in combination with the *F deletions. No wing defects or female sterility was seen in *F zf30CEP518/zf30Ck02506 flies. zf30CEP2228/zf30Ck02506 flies showed *F only weakly penetrant L5 shortening or thinning, and females were fertile. *F Consistent with the BDGP results, the chromosome carrying *F P{lacW}zf30Ck02506 was lethal in combination with Df(2L)N22-5. It was *F also lethal with Df(2L)N22-14. This indicates the presence of at least one *F lethal mutation linked to the P{lacW}zf30Ck02506 insertion. The l(2)DB2 *F locus is probably mutated, since l(2)DB21 failed to complement the *F chromosome carrying P{lacW}zf30Ck02506. *F Dp(2;Y)cb50 was capable of at least partially rescuing the wing phenotypes *F of the zf30C alleles in combination with Df(2L)s1402. Dp(2;Y)cb50; *F zf30Ck02506/Df(2L)s1402 males were normal. Dp(2;Y)cb50; *F zf30CEP518/Df(2L)s1402 males had normal wing veins, but extremely *F outspread wings. Dp(2;Y)cb50; zf30CEP2228/Df(2L)s1402 males showed a *F weakly penetrant vein shortening phenotype, and outspread wings. Curiously, *F the outspread wing phenotype was more penetrant and pronounced in the *F presence of the duplication than in its absence. *F Loss-of-function alleles in the nearby und locus show a L5 shortening *F phenotype similar to that of zf30C alleles. Nevertheless, complementation *F tests between zf30Ck02506, zf30CEP518 or zf30CEP2228 and unddelta34 *F showed no abnormal phenotypes. *F References: *F Adams et al., 2000. The genome sequence of Drosophila melanogaster. Science *F 287: 2185-2195 (FBrf0126983). *F Castrillon et al., 1993. Toward a molecular genetic analysis of *F spermatogenesis in Drosophila melanogaster: characterization of *F male-sterile mutants generated by single P element mutagenesis. Genetics *F 135: 489-505 (FBrf0064394). *F Cutforth and Gaul, 1999. A methionine aminopeptidase and putative regulator *F of translation initiation is required for cell growth and patterning in *F Drosophila. Mech. Dev. 82(1,2): 23--28 (FBrf0108434). *F Spradling et al., 1999. The Berkeley Drosophila genome project gene *F disruption project. Single P-element insertions mutating 25% of vital *F Drosophila genes. Genetics 153: 135-177 (FBrf0111489) *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0145658 *a Ren *b X. *t 2002.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:54:52 2002 *F To: xren@nature.berkeley.edu *F Subject: Helping FlyBase: CSH-45 *F Dear Xiaoyun, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The jitterbug gene: a mutation in a Drosophila filamin causes seizures, *F synaptic failure and paralysis'. *F You mention a gene that is relatively new to FlyBase, jbug. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F You mention a P-element insertion allele of jbug. Is this one of the *F BDGP P insertions? Is it the same allele that is currently represented *F in FlyBase as jbug1 (FBal0100359)? We have insertion records for all *F of the major collections and we like to keep these tied to alleles as *F those alleles are identified. *F Finally \- we do have a gene record for a Filamin at 59A (FBgn0041831). *F Do you know whether this is the same gene as jbug? *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From unaren@uclink.berkeley.edu Fri Mar 08 17:18:12 2002 *F To: Rachel Drysdale (Genetics) , xren@nature.berkeley.edu *F Subject: Re: Helping FlyBase: CSH-45 *F Hi, *F I included answers to some of your questions below. I am no longer on this *F project. If you need more info, please ask Mark Tanouye who is the head of *F the lab (tanouye@uclink4.berkeley.edu). *F >You mention a gene that is relatively new to FlyBase, jbug. Do you> *F >know which of the Genome Project CG annotations your gene corresponds *F >to? *F CG3550, CG11605 and CG13525. *F >You mention a P-element insertion allele of jbug. Is this one of the *F >BDGP P insertions? Is it the same allele that is currently represented *F >in FlyBase as jbug1 (FBal0100359)? We have insertion records for all *F >of the major collections and we like to keep these tied to alleles as *F >those alleles are identified. *F This P-element was generated in our lab. *F >Finally \- we do have a gene record for a Filamin at 59A (FBgn0041831). *F >Do you know whether this is the same gene as jbug? *F yes. *F hope this helps. *F \-una # *U FBrf0145659 *a Bashaw *b G. *t 2002.3.8 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 16:13:37 2002 *F To: gbashaw@mail.med.upenn.edu *F Subject: Helping FlyBase: CSH-205 *F Dear Greg, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A novel Dbl family Rho-GEF promotes axon attraction *F to the cns midline and overcomes robo repulsion.' *F You mention a gene that is new to FlyBase, GEF64C. Do you know which *F of the Genome Project CG annotations your gene corresponds to? Might *F it be CG13709? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. We would of course *F rename CG13709 if it is your GEF64C. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From gbashaw@mail.med.upenn.edu Fri Mar 08 17:37:00 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-205 *F Hi Rachel, *F GEF64C cDNAs appear to be comprised of two different CG#s *F I'm not sure what this means, but we had the same experience with the *F highwire gene. *F The business end (i.e. the part with the Dbl GEF domain) corresponds *F to the CG13709 as you indicated in your e-mail. *F The N- teminus corresponds to the neighbouring CG13710 *F Best *F Greg *F \-- *F Greg J. Bashaw Ph.D. *F Assistant Professor *F Department of Neuroscience *F University of Pennsylvania School of Medicine *F 1113 BRBII/III *F 421 Curie Blvd. *F Philadelphia, PA 19104 *F Phone: 215-898-0829 *F FAX: 215-573-7601 *F Email: gbashaw@mail.med.upenn.edu # *U FBrf0145660 *a Seeger *b M. *t 2002.3.9 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:55:32 2002 *F To: seeger.9@osu.edu *F Subject: Helping FlyBase: CSH-86 *F Dear Mark, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Mechanisms of commissureless function in regulating axon guidance at *F the CNS midline.' *F You mention two genes comm2 and comm3 that are relatively new and new *F to FlyBase, respectively. Do you know which of the Genome Project CG *F annotations your gene corresponds to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From seeger.9@osu.edu Sat Mar 09 15:45:19 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-86 *F Rachel \- *F Comm2 is CG7554 *F Comm3 is CG13447 and CG13448. The genome project has the two different *F exons of comm3 as two separate CG \#s and not completely accurate. The open *F reading frame for Comm 3 is as indicated below: *F MADLLKALNITGNLLAGLEDAGNLTIATATTTTLSEGLNGNATTLDAAASTSSGEKLDLGGFKLPGNYSILINKLEQLAL *F GLDSALGNFTIDRQEVEINLSGAATANDVADVADDLFDVLPAARAPPPHIAHGSRIYTGDDGQDFAHVVSDIWIGVILTL *F LIVFVIFFICACFVYHKFQQWKNSYRANHSdptieicrrcppdyeaeslpsytivsglptyddaleefrkagiiltpamv *F piikifecnetgkeqavgyslvetniasadaaadnvslasttcncgnssptsslpsysaataavmaaaaaappqvielsp *F ehlaslsqkrlslqisfnnsvrrqsplrnhllrsravgntagvgpseaagpaihgsgahgvhggggatilpvlhrsssti *F slsnerqlldqrlrhlhhrgsly *F Cheers, *F Mark *F Mark A. Seeger, Ph.D. *F Department of Molecular Genetics and *F the Neurobiotechnology Center *F The Ohio State University *F 125 Rightmire Hall *F 1060 Carmack Road *F Columbus, OH 43210 *F 614/292-5106 (office) 292-5107 (lab) 292-5379 (fax) # *U FBrf0145661 *a Phillips *b M. *t 2002.3.21 *T personal communication to FlyBase *u *F Date: Fri, 8 Feb 2002 16:29:20 -0500 (EST) *F From: fbmailer@bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail *F Mime-Version: 1.0 *F *F comments: To whom it may concern: *F I recently downloaded your sequence for the UAS cloning vector pGaTB to compare to *F sequence from our facility. There are some small discrepancies between my sequence and *F yours: CGGATCCGCGAAAGCTAAGCAAATA (Flybase position 2855-2880) has been replaced *F by GATCCTCTAGGGTACG (my sequence); and there are two small insertions flanking AAGCTT *F (Flybase position 2916-2921) so it now sequences as CCCAAGCTTGAAG (my sequence). *F *F Thanks for your help, *F Matt Phillips *F mailto: flybase-help@morgan.harvard.edu *F realname: Matt Phillips *F reply-to: mdp163@psu.edu # *U FBrf0146674 *a Roth *b F. *c R.E. *d Foulger *t 2002.4.24 *T personal communication to FlyBase *u *F All of Fritz Roth's predicted GO terms (table following) have been *F analysed based on existing GO terms for the genes, and additional *F information recorded in the genes database. Predicted terms that were *F scored 'true' and were more specific than the existing GO terms were *F included in the gene records. GO terms that were considered too broad *F and therefore uninformative have not been included. *F ===================================================================== *F NEW HYPOTHESES *F Attribute_ID Attribute_Description Gene-Rank KLI UPD_Prob DEV_Prob Gene_ID Odds_Factor1 Attribute_Description Odds_Factor2 Attribute_Description Odds_factor3 Attribute_Description *F 3824 F-enzyme 1 6.79847 0.873373 0.00293765 FBgn0035076 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 16787 F-hydrolase 1 4.84856 0.552503 0.000657658 FBgn0035076 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 5525 F-GTP binding 1 4.82579 0.975532 0.0288403 FBgn0024947 32.96 F-hydrolase, acting on acid anhydrides 32.96 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 19001 F-guanyl nucleotide binding 1 4.81171 0.975423 0.0291072 FBgn0024947 32.74 F-hydrolase, acting on acid anhydrides 32.74 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 16181 P-synaptic vesicle transport/synaptic vesicle recycling 1 4.56971 0.934029 0.0260016 FBgn0001219 25.37 P-neurotransmitter release 25.37 P-neurotransmitter maintenance 0.89 P-response to abiotic stimulus *F 5525 F-GTP binding 2 4.41924 0.932778 0.0288403 FBgn0016687 20.04 F-hydrolase, acting on acid anhydrides 20.04 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 19001 F-guanyl nucleotide binding 2 4.40541 0.932612 0.0291072 FBgn0016687 19.92 F-hydrolase, acting on acid anhydrides 19.92 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 4930 F-G-protein coupled receptor/G-protein linked receptor 1 4.20278 0.98948 0.0496592 FBgn0004625 90.34 P-G-protein coupled receptor protein signaling pathway 7.83 P-perception of abiotic stimulus 6.19 P-perception of chemical substance *F 19226 P-transmission of nerve impulse 1 4.19939 0.902197 0.0279254 FBgn0026634 332.28 P-vesicle transport 0.77 P-intracellular protein traffic 0.79 C-cellular_component *F 7268 P-synaptic transmission 1 4.19939 0.902197 0.0279254 FBgn0026634 332.28 P-vesicle transport 0.77 P-intracellular protein traffic 0.79 C-cellular_component *F 7267 P-cell-cell signaling 1 4.18186 0.901525 0.0281838 FBgn0026634 327.86 P-vesicle transport 0.76 P-intracellular protein traffic 0.79 C-cellular_component *F 5525 F-GTP binding 3 4.17746 0.904594 0.0288403 FBgn0025115 15.77 F-hydrolase, acting on acid anhydrides 15.77 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 19001 F-guanyl nucleotide binding 3 4.16486 0.90452 0.0291072 FBgn0025115 15.68 F-hydrolase, acting on acid anhydrides 15.68 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 6952 P-defense response 1 4.16422 0.937991 0.0360579 FBgn0011598 1380.72 P-stress response 0.02 P-phosphate metabolism 31.01 P-phosphorylation *F 16192 P-vesicle transport 1 4.08714 0.928979 0.0360579 FBgn0001219 24.35 P-neurotransmitter release 24.35 P-neurotransmitter maintenance 0.82 P-cell-cell signaling *F 7166 P-cell surface receptor linked signal transduction 1 4.06941 0.979999 0.0509331 FBgn0025680 51.67 P-perception of abiotic stimulus 34.11 F-G-protein coupled receptor/G-protein linked receptor 3.92 C-cell *F 5681 C-spliceosome 1 4.00051 0.867954 0.0262422 FBgn0010252 226871.77 P-RNA splicing 0 P-mRNA splicing 0.39 F-RNA binding *F 6886 P-intracellular protein traffic 1 3.99764 0.926366 0.0379315 FBgn0001219 25.33 P-neurotransmitter release 25.33 P-neurotransmitter maintenance 0.78 P-cell-cell signaling *F 15031 P-protein transport 1 3.82271 0.916899 0.0408319 FBgn0001219 24.94 P-neurotransmitter release 24.94 P-neurotransmitter maintenance 0.81 P-cell-cell signaling *F 5681 C-spliceosome 2 3.81862 0.844605 0.0262422 FBgn0004587 150250.07 P-RNA splicing 0 P-mRNA splicing 0.44 F-RNA binding *F 9593 P-perception of chemical substance 1 3.79321 0.839819 0.0260016 FBgn0004784 224.07 P-sensory perception 0.6 P-phosphorylation 1.51 P-phosphate metabolism *F 7606 P-chemosensory perception 1 3.77259 0.837107 0.0260016 FBgn0025680 218.09 F-G-protein coupled receptor/G-protein linked receptor 1.51 F-receptor 0.74 F-signal transducer *F 6952 P-defense response 2 3.60711 0.868298 0.0360579 FBgn0020238 85.14 P-stress response 2.68 P-signal transduction 0.43 P-cell cycle/cell-division cycle *F 7165 P-signal transduction 1 3.56901 0.964833 0.0659174 FBgn0025680 36.27 P-perception of abiotic stimulus 15.45 F-G-protein coupled receptor/G-protein linked receptor 0.17 P-perception of external stimulus *F 7345 P-embryogenesis and morphogenesis 1 3.5659 0.942014 0.057544 FBgn0023423 174.48 P-cell communication 0.04 P-signal transduction 19.61 P-cell surface receptor linked signal transduction *F 4674 F-protein serine/threonine kinase 1 3.42989 0.887847 0.0465586 FBgn0015295 127.48 P-protein amino acid phosphorylation 2.34 P-phosphate metabolism 0.48 P-phosphorylation *F 4674 F-protein serine/threonine kinase 2 3.42989 0.887847 0.0465586 FBgn0013955 127.48 P-protein amino acid phosphorylation 2.34 P-phosphate metabolism 0.48 P-phosphorylation *F 4674 F-protein serine/threonine kinase 3 3.42989 0.887847 0.0465586 FBgn0014073 127.48 P-protein amino acid phosphorylation 2.34 P-phosphate metabolism 0.48 P-phosphorylation *F 15630 C-microtubule cytoskeleton 1 3.4261 0.818632 0.0310456 FBgn0040395 61.91 P-microtubule-based process 2.19 P-developmental processes 0.74 P-organelle organization and biogenesis *F 9593 P-perception of chemical substance 2 3.41823 0.788904 0.0260016 FBgn0000121 224.07 P-sensory perception 0.67 C-cell 0.78 C-plasma membrane/cell membrane *F 15630 C-microtubule cytoskeleton 2 3.37248 0.811082 0.0310456 FBgn0024187 61.91 P-microtubule-based process 2.19 P-developmental processes 0.74 P-organelle organization and biogenesis *F 6810 P-transport 1 3.32821 0.91441 0.0586138 FBgn0001219 21.18 P-neurotransmitter release 21.18 P-neurotransmitter maintenance 0.69 F-chaperone *F 7186 P-G-protein coupled receptor protein signaling pathway 1 3.31646 0.795201 0.0295801 FBgn0003444 46.62 F-G-protein coupled receptor/G-protein linked receptor 3.56 C-cell 0.6 F-transmembrane receptor *F 5856 C-cytoskeleton 1 3.31382 0.870177 0.0461318 FBgn0028388 13.86 P-microtubule-based process 4.02 P-cytoskeleton organization and biogenesis 3.09 P-developmental processes *F 4888 F-transmembrane receptor 1 3.23351 0.986359 0.0959401 FBgn0004625 292.7 P-perception of external stimulus 0.01 P-perception of abiotic stimulus 11.7 P-G-protein coupled receptor protein signaling pathway *F 19226 P-transmission of nerve impulse 2 3.2207 0.772089 0.0279254 FBgn0033714 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7268 P-synaptic transmission 2 3.2207 0.772089 0.0279254 FBgn0033714 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 5681 C-spliceosome 3 3.21242 0.760983 0.0262422 FBgn0028577 47073 P-RNA splicing 0 P-mRNA splicing 0.33 F-RNA binding *F 7397 P-histogenesis and organogenesis 1 3.20973 0.854542 0.0459198 FBgn0023423 285.58 P-cell communication 0.01 P-signal transduction 15.6 P-cell surface receptor linked signal transduction *F 19226 P-transmission of nerve impulse 3 3.2088 0.770358 0.0279254 FBgn0025725 166.54 P-vesicle transport 0.79 P-intracellular protein traffic 0.83 C-cellular_component *F 7268 P-synaptic transmission 3 3.2088 0.770358 0.0279254 FBgn0025725 166.54 P-vesicle transport 0.79 P-intracellular protein traffic 0.83 C-cellular_component *F 6371 P-mRNA splicing 1 3.2026 0.774645 0.0288403 FBgn0036548 68.42 C-spliceosome 1.28 F-RNA binding 1.16 F-nucleic acid binding *F 7267 P-cell-cell signaling 2 3.19836 0.770316 0.0281838 FBgn0025725 164.59 P-vesicle transport 0.78 P-intracellular protein traffic 0.84 C-cellular_component *F 7267 P-cell-cell signaling 3 3.19794 0.770254 0.0281838 FBgn0033714 118.08 P-vesicle transport 0.8 P-intracellular protein traffic 1.16 P-protein transport *F 19226 P-transmission of nerve impulse 4 3.19332 0.768101 0.0279254 FBgn0038637 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7268 P-synaptic transmission 4 3.19332 0.768101 0.0279254 FBgn0038637 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 4674 F-protein serine/threonine kinase 4 3.18873 0.853948 0.0465586 FBgn0022800 127.48 P-protein amino acid phosphorylation 2.34 P-phosphate metabolism 0.48 P-phosphorylation *F 19226 P-transmission of nerve impulse 5 3.17782 0.765835 0.0279254 FBgn0022268 235.2 P-vesicle transport 0.72 C-cellular_component 0.78 P-intracellular protein traffic *F 7268 P-synaptic transmission 5 3.17782 0.765835 0.0279254 FBgn0022268 235.2 P-vesicle transport 0.72 C-cellular_component 0.78 P-intracellular protein traffic *F 7267 P-cell-cell signaling 4 3.16994 0.766159 0.0281838 FBgn0038637 118.08 P-vesicle transport 0.8 P-intracellular protein traffic 1.16 P-protein transport *F 19226 P-transmission of nerve impulse 6 3.1682 0.764425 0.0279254 FBgn0028538 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7268 P-synaptic transmission 6 3.1682 0.764425 0.0279254 FBgn0028538 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7398 P-ectoderm development 1 3.16686 0.821767 0.0394457 FBgn0003391 157.33 P-cell communication 0.26 C-integral plasma membrane protein 2.61 C-plasma membrane/cell membrane *F 19226 P-transmission of nerve impulse 7 3.16341 0.763724 0.0279254 FBgn0040087 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7268 P-synaptic transmission 7 3.16341 0.763724 0.0279254 FBgn0040087 119.38 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7267 P-cell-cell signaling 5 3.14976 0.763194 0.0281838 FBgn0040087 118.08 P-vesicle transport 0.8 P-intracellular protein traffic 1.16 P-protein transport *F 7267 P-cell-cell signaling 6 3.14925 0.76312 0.0281838 FBgn0022268 232.26 P-vesicle transport 0.72 C-cellular_component 0.77 P-intracellular protein traffic *F 7267 P-cell-cell signaling 7 3.1457 0.762598 0.0281838 FBgn0028538 118.08 P-vesicle transport 0.8 P-intracellular protein traffic 1.16 P-protein transport *F 5524 F-ATP binding 1 3.12286 0.93156 0.0753356 FBgn0024947 12.2 F-hydrolase, acting on acid anhydrides 12.2 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.9 F-hydrolase *F 7397 P-histogenesis and organogenesis 2 3.10383 0.839069 0.0459198 FBgn0003391 149.47 P-cell communication 0.22 C-integral plasma membrane protein 2.99 C-plasma membrane/cell membrane *F 7275 P-developmental processes 1 3.07216 0.973245 0.0990832 FBgn0023423 67.11 P-cell communication 24.09 P-cell surface receptor linked signal transduction 0.1 P-signal transduction *F 4674 F-protein serine/threonine kinase 5 3.06683 0.83603 0.0465586 FBgn0020391 153.24 P-protein amino acid phosphorylation 2.42 P-phosphate metabolism 0.43 F-transmembrane receptor *F 7398 P-ectoderm development 2 3.06069 0.806003 0.0394457 FBgn0023423 302.6 P-cell communication 0.02 P-signal transduction 10.87 P-cell surface receptor linked signal transduction *F 15630 C-microtubule cytoskeleton 3 3.01719 0.759111 0.0310456 FBgn0024180 208.25 P-microtubule-based process 0.29 P-M phase 2.15 P-nuclear division *F 5875 C-microtubule associated protein 1 3.00257 0.724211 0.025293 FBgn0036882 22.75 P-microtubule-based process 2.56 P-developmental processes 1.55 F-protein binding *F 5875 C-microtubule associated protein 2 3.00257 0.724211 0.025293 FBgn0021825 22.75 P-microtubule-based process 2.56 P-developmental processes 1.55 F-protein binding *F 7186 P-G-protein coupled receptor protein signaling pathway 2 2.9944 0.747796 0.0295801 FBgn0025680 157.36 F-G-protein coupled receptor/G-protein linked receptor 0.25 C-intracellular/protoplasm 3.76 C-cell *F 6396 P-RNA processing 1 2.98896 0.782119 0.0365595 FBgn0036548 44.35 C-spliceosome 1.38 F-RNA binding 1.24 F-nucleic acid binding *F 16070 P-RNA metabolism 1 2.97559 0.783219 0.0372392 FBgn0036548 43.73 C-spliceosome 1.39 F-RNA binding 1.25 F-nucleic acid binding *F 4674 F-protein serine/threonine kinase 6 2.97196 0.821727 0.0465586 FBgn0024245 127.48 P-protein amino acid phosphorylation 2.34 P-phosphate metabolism 0.48 P-phosphorylation *F 5875 C-microtubule associated protein 3 2.9625 0.718176 0.025293 FBgn0040228 22.75 P-microtubule-based process 2.56 P-developmental processes 1.5 F-protein binding *F 5875 C-microtubule associated protein 4 2.9625 0.718176 0.025293 FBgn0001108 22.75 P-microtubule-based process 2.56 P-developmental processes 1.5 F-protein binding *F 9605 P-response to external stimulus 1 2.96059 0.949426 0.0937562 FBgn0003444 17.3 F-G-protein coupled receptor/G-protein linked receptor 2.43 P-cell surface receptor linked signal transduction 2.38 C-cell *F 19226 P-transmission of nerve impulse 8 2.96035 0.733398 0.0279254 FBgn0013726 119.38 P-vesicle transport 0.76 P-M phase 0.8 P-intracellular protein traffic *F 7268 P-synaptic transmission 8 2.96035 0.733398 0.0279254 FBgn0013726 119.38 P-vesicle transport 0.76 P-M phase 0.8 P-intracellular protein traffic *F 7267 P-cell-cell signaling 8 2.95148 0.733516 0.0281838 FBgn0013726 118.08 P-vesicle transport 0.75 P-M phase 0.8 P-intracellular protein traffic *F 5525 F-GTP binding 4 2.95127 0.737166 0.0288403 FBgn0004402 8.44 F-hydrolase, acting on acid anhydrides 8.44 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 19001 F-guanyl nucleotide binding 4 2.94445 0.737604 0.0291072 FBgn0004402 8.4 F-hydrolase, acting on acid anhydrides 8.4 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 15630 C-microtubule cytoskeleton 4 2.93986 0.747348 0.0310456 FBgn0026193 208.25 P-microtubule-based process 0.01 P-phosphate metabolism 102 P-phosphorylation *F 5743 C-mitochondrial inner membrane/cristae 1 2.93553 0.724155 0.0269774 FBgn0032833 46.92 F-primary active transporter 1.82 F-carrier/carrier type transporter 0.84 F-cation transporter *F 5743 C-mitochondrial inner membrane/cristae 2 2.93553 0.724155 0.0269774 FBgn0033020 46.92 F-primary active transporter 1.82 F-carrier/carrier type transporter 0.84 F-cation transporter *F 7600 P-sensory perception 1 2.93185 0.759417 0.0336512 FBgn0014019 111.93 P-perception of abiotic stimulus 3.59 P-G-protein coupled receptor protein signaling pathway 0.36 F-G-protein coupled receptor/G-protein linked receptor *F 6950 P-stress response 1 2.92017 0.732413 0.0288403 FBgn0043901 48.15 P-response to external stimulus 1.38 P-cell communication 1.15 P-perception of abiotic stimulus *F 15405 F-P-P-bond-hydrolyzis-driven transporter 1 2.91021 0.761859 0.0348337 FBgn0017646 46.05 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 1.71 F-ATPase 1.14 F-ATP binding *F 9605 P-response to external stimulus 2 2.88356 0.938953 0.0937562 FBgn0024836 7.75 F-G-protein coupled receptor/G-protein linked receptor 3.62 C-cell 0.39 C-intracellular/protoplasm *F 5875 C-microtubule associated protein 5 2.88307 0.706095 0.025293 FBgn0033206 22.75 P-microtubule-based process 2.56 P-developmental processes 1.39 F-protein binding *F 5875 C-microtubule associated protein 6 2.88307 0.706095 0.025293 FBgn0032761 22.75 P-microtubule-based process 2.56 P-developmental processes 1.39 F-protein binding *F 5875 C-microtubule associated protein 7 2.88307 0.706095 0.025293 FBgn0010622 22.75 P-microtubule-based process 2.56 P-developmental processes 1.39 F-protein binding *F 4674 F-protein serine/threonine kinase 7 2.87335 0.806526 0.0465586 FBgn0011829 127.48 P-protein amino acid phosphorylation 2.34 P-phosphate metabolism 0.43 F-transmembrane receptor *F 6371 P-mRNA splicing 2 2.85263 0.722003 0.0288403 FBgn0035843 68.42 C-spliceosome 1.1 F-nucleic acid binding 1.09 C-nucleus *F 5654 C-nucleoplasm 1 2.85125 0.779063 0.0406443 FBgn0041186 35.81 P-mRNA processing 0.14 P-RNA metabolism 3.98 P-cell cycle/cell-division cycle *F 5654 C-nucleoplasm 2 2.84944 0.77878 0.0406443 FBgn0030505 9.57 P-transcription 2.25 P-metabolism 1.97 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 6371 P-mRNA splicing 3 2.84138 0.720257 0.0288403 FBgn0036683 68.42 C-spliceosome 1.1 F-nucleic acid binding 1.09 C-nucleus *F 5856 C-cytoskeleton 2 2.82236 0.796882 0.0461318 FBgn0024923 13.86 P-microtubule-based process 3.19 P-cell growth and/or maintenance 0.41 P-gametogenesis *F 5856 C-cytoskeleton 3 2.80737 0.794515 0.0461318 FBgn0024180 30.03 P-microtubule-based process 0.25 P-M phase 3.06 P-cytoskeleton organization and biogenesis *F 3824 F-enzyme 2 2.78943 0.911444 0.0870964 FBgn0030741 0.59 P-phosphate metabolism 1.58 F-ligand binding or carrier 1.4 P-phosphorylation *F 7345 P-embryogenesis and morphogenesis 2 2.78497 0.831456 0.057544 FBgn0003391 96.62 P-cell communication 0.21 C-integral plasma membrane protein 3.68 C-plasma membrane/cell membrane *F 5856 C-cytoskeleton 4 2.77748 0.78977 0.0461318 FBgn0040395 13.86 P-microtubule-based process 2.33 P-cytoskeleton organization and biogenesis 1.67 P-developmental processes *F 16787 F-hydrolase 2 2.77547 0.660677 0.0208449 FBgn0030741 1.69 F-ligand binding or carrier 0.62 P-phosphate metabolism 1.4 P-phosphorylation *F 4674 F-protein serine/threonine kinase 8 2.76533 0.789484 0.0465586 FBgn0004864 37.91 P-protein amino acid phosphorylation 0.53 P-response to external stimulus 1.84 P-phosphate metabolism *F 16791 F-phosphoric monoester hydrolase 1 2.74428 0.77016 0.0425598 FBgn0013310 65.14 F-phosphatase 0.98 P-phosphate metabolism 1.01 P-phosphorylation *F 5856 C-cytoskeleton 5 2.73314 0.782675 0.0461318 FBgn0013733 12.52 P-microtubule-based process 2.25 P-cytoskeleton organization and biogenesis 1.97 F-protein binding *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 1 2.71299 0.691051 0.027227 FBgn0015776 62.37 F-P-P-bond-hydrolyzis-driven transporter 0.54 C-integral plasma membrane protein 0.63 C-cell *F 4674 F-protein serine/threonine kinase 9 2.7116 0.780854 0.0465586 FBgn0010407 127.48 P-protein amino acid phosphorylation 0.32 F-transmembrane receptor 2.34 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 1 2.70992 0.88776 0.0820352 FBgn0032811 70.66 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 2 2.70992 0.88776 0.0820352 FBgn0003075 70.66 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 6371 P-mRNA splicing 4 2.69974 0.697984 0.0288403 FBgn0039558 68.42 C-spliceosome 1.09 C-nucleus 0.97 C-intracellular/protoplasm *F 4674 F-protein serine/threonine kinase 10 2.69765 0.778597 0.0465586 FBgn0020440 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 5856 C-cytoskeleton 6 2.69555 0.776605 0.0461318 FBgn0024187 13.86 P-microtubule-based process 2.33 P-cytoskeleton organization and biogenesis 1.67 P-developmental processes *F 5524 F-ATP binding 2 2.68526 0.867233 0.0753356 FBgn0016687 8.55 F-hydrolase, acting on acid anhydrides 8.55 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.92 F-hydrolase *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 2 2.68516 0.686637 0.027227 FBgn0015777 62.37 F-P-P-bond-hydrolyzis-driven transporter 0.54 C-integral plasma membrane protein 0.56 C-cell *F 15630 C-microtubule cytoskeleton 5 2.68243 0.706996 0.0310456 FBgn0040207 52.8 P-microtubule-based process 1.52 F-protein binding 0.75 P-organelle organization and biogenesis *F 15630 C-microtubule cytoskeleton 6 2.68243 0.706996 0.0310456 FBgn0040208 52.8 P-microtubule-based process 1.52 F-protein binding 0.75 P-organelle organization and biogenesis *F 3723 F-RNA binding 1 2.68048 0.833509 0.0635331 FBgn0035253 1108.29 P-RNA splicing 0.01 P-mRNA splicing 2.38 P-mRNA processing *F 4674 F-protein serine/threonine kinase 11 2.67944 0.77564 0.0465586 FBgn0011568 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 12 2.67944 0.77564 0.0465586 FBgn0010335 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 13 2.67944 0.77564 0.0465586 FBgn0010336 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 14 2.67944 0.77564 0.0465586 FBgn0010337 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 15 2.67944 0.77564 0.0465586 FBgn0004601 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 16 2.67944 0.77564 0.0465586 FBgn0004602 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 17 2.67944 0.77564 0.0465586 FBgn0004604 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 4674 F-protein serine/threonine kinase 18 2.67944 0.77564 0.0465586 FBgn0020029 57.09 P-protein amino acid phosphorylation 2 P-phosphate metabolism 0.55 P-phosphorylation *F 5875 C-microtubule associated protein 8 2.67219 0.673252 0.025293 FBgn0040395 25.8 P-microtubule-based process 2.7 P-developmental processes 0.8 P-organelle organization and biogenesis *F 5525 F-GTP binding 5 2.66774 0.692878 0.0288403 FBgn0037900 7.54 F-hydrolase, acting on acid anhydrides 7.54 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 5525 F-GTP binding 6 2.66774 0.692878 0.0288403 FBgn0031700 7.54 F-hydrolase, acting on acid anhydrides 7.54 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 3735 F-structural protein of ribosome/ribosomal protein 1 2.66549 0.732418 0.0368129 FBgn0015806 87.91 C-ribosome 0.88 F-protein serine/threonine kinase 0.91 P-phosphate metabolism *F 19001 F-guanyl nucleotide binding 5 2.6624 0.693478 0.0291072 FBgn0037900 7.51 F-hydrolase, acting on acid anhydrides 7.51 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 19001 F-guanyl nucleotide binding 6 2.6624 0.693478 0.0291072 FBgn0031700 7.51 F-hydrolase, acting on acid anhydrides 7.51 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 1.22 F-ATPase *F 5654 C-nucleoplasm 3 2.63792 0.744879 0.0406443 FBgn0015949 31.14 P-mRNA processing 0.23 P-RNA metabolism 3.2 F-transferase, transferring phosphorus-containing groups *F 3735 F-structural protein of ribosome/ribosomal protein 2 2.63368 0.727241 0.0368129 FBgn0035422 33.23 C-ribosome 1.58 P-protein biosynthesis/translation 1.17 C-cytosolic ribosome *F 3735 F-structural protein of ribosome/ribosomal protein 3 2.63368 0.727241 0.0368129 FBgn0032987 33.23 C-ribosome 1.58 P-protein biosynthesis/translation 1.17 C-cytosolic ribosome *F 5840 C-ribosome 1 2.62381 0.73621 0.0391742 FBgn0037566 56.69 F-structural protein of ribosome/ribosomal protein 1.18 P-protein biosynthesis/translation 0.95 P-protein metabolism and modification *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 1 2.6207 0.910855 0.0988553 FBgn0038367 18.68 C-nucleoplasm 2.71 F-DNA binding 1.6 C-intracellular/protoplasm *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 2 2.6207 0.910855 0.0988553 FBgn0033183 18.68 C-nucleoplasm 2.71 F-DNA binding 1.6 C-intracellular/protoplasm *F 5654 C-nucleoplasm 4 2.61572 0.741235 0.0406443 FBgn0019938 8.19 P-transcription 2.01 P-metabolism 1.85 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 5386 F-carrier/carrier type transporter 1 2.61336 0.906062 0.097051 FBgn0037710 16.37 C-mitochondrial membrane 5.44 P-protein transport 0.72 P-organelle organization and biogenesis *F 5875 C-microtubule associated protein 9 2.60508 0.662559 0.025293 FBgn0024187 25.8 P-microtubule-based process 2.7 P-developmental processes 0.8 P-organelle organization and biogenesis *F 7275 P-developmental processes 2 2.60388 0.908734 0.0990832 FBgn0015589 10.45 P-microtubule-based process 4.24 P-cell surface receptor linked signal transduction 0.29 P-cell organization and biogenesis *F 6396 P-RNA processing 2 2.59925 0.720437 0.0365595 FBgn0036683 44.35 C-spliceosome 1.22 C-nucleus 1.15 F-nucleic acid binding *F 6468 P-protein amino acid phosphorylation 1 2.59459 0.784773 0.0528445 FBgn0020621 45.03 F-protein kinase 1.39 F-protein serine/threonine kinase 0.92 F-enzyme *F 19226 P-transmission of nerve impulse 9 2.58997 0.675306 0.0279254 FBgn0028969 119.38 P-vesicle transport 0.72 C-cellular_component 0.8 P-intracellular protein traffic *F 7268 P-synaptic transmission 9 2.58997 0.675306 0.0279254 FBgn0028969 119.38 P-vesicle transport 0.72 C-cellular_component 0.8 P-intracellular protein traffic *F 6396 P-RNA processing 3 2.58989 0.718896 0.0365595 FBgn0035843 44.35 C-spliceosome 1.22 C-nucleus 1.15 F-nucleic acid binding *F 16070 P-RNA metabolism 2 2.58625 0.721403 0.0372392 FBgn0036683 43.73 C-spliceosome 1.22 C-nucleus 1.15 F-nucleic acid binding *F 7267 P-cell-cell signaling 9 2.58616 0.676124 0.0281838 FBgn0028969 118.08 P-vesicle transport 0.72 C-cellular_component 0.8 P-intracellular protein traffic *F 16070 P-RNA metabolism 3 2.58013 0.720394 0.0372392 FBgn0035843 43.73 C-spliceosome 1.22 C-nucleus 1.15 F-nucleic acid binding *F 15630 C-microtubule cytoskeleton 7 2.57575 0.68972 0.0310456 FBgn0035800 52.8 P-microtubule-based process 0.75 P-organelle organization and biogenesis 1.32 F-structural protein *F 5654 C-nucleoplasm 5 2.56362 0.732621 0.0406443 FBgn0000099 10.26 P-transcription 2.37 P-metabolism 2.03 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 280 P-nuclear division 1 2.56082 0.654057 0.0250611 FBgn0040080 34.64 P-DNA replication and chromosome cycle 0.12 P-cytoplasm organization and biogenesis 6.11 P-cell organization and biogenesis *F 5740 C-mitochondrial membrane 1 2.55646 0.728251 0.0399025 FBgn0032833 40.07 F-carrier/carrier type transporter 1.15 F-primary active transporter 1.12 P-intracellular protein traffic *F 5740 C-mitochondrial membrane 2 2.55646 0.728251 0.0399025 FBgn0033020 40.07 F-carrier/carrier type transporter 1.15 F-primary active transporter 1.12 P-intracellular protein traffic *F 16791 F-phosphoric monoester hydrolase 2 2.55085 0.738508 0.0425598 FBgn0033075 54.91 F-phosphatase 0.98 P-phosphate metabolism 1.01 P-phosphorylation *F 3824 F-enzyme 3 2.54389 0.873373 0.0870964 FBgn0030370 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 4 2.54389 0.873373 0.0870964 FBgn0032535 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 5 2.54389 0.873373 0.0870964 FBgn0039049 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 6 2.54389 0.873373 0.0870964 FBgn0039050 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 7 2.54389 0.873373 0.0870964 FBgn0039051 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 8 2.54389 0.873373 0.0870964 FBgn0039052 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 9 2.54389 0.873373 0.0870964 FBgn0039053 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 10 2.54389 0.873373 0.0870964 FBgn0034088 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 11 2.54389 0.873373 0.0870964 FBgn0033366 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 12 2.54389 0.873373 0.0870964 FBgn0029805 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 13 2.54389 0.873373 0.0870964 FBgn0031342 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 14 2.54389 0.873373 0.0870964 FBgn0035781 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 15 2.54389 0.873373 0.0870964 FBgn0037396 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 16 2.54389 0.873373 0.0870964 FBgn0038899 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 17 2.54389 0.873373 0.0870964 FBgn0033874 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 3824 F-enzyme 18 2.54389 0.873373 0.0870964 FBgn0030223 0.59 P-phosphate metabolism 1.4 P-phosphorylation 0.85 F-purine nucleotide binding *F 9605 P-response to external stimulus 3 2.53749 0.887206 0.0937562 FBgn0023000 0.03 P-G-protein coupled receptor protein signaling pathway 32 P-stress response 11.58 F-G-protein coupled receptor/G-protein linked receptor *F 5856 C-cytoskeleton 7 2.53624 0.750321 0.0461318 FBgn0035800 12.52 P-microtubule-based process 2.25 P-cytoskeleton organization and biogenesis 1.59 F-structural protein *F 6950 P-stress response 2 2.53448 0.671296 0.0288403 FBgn0000462 226.85 P-response to external stimulus 0.16 P-defense response 0.5 P-signal transduction *F 7275 P-developmental processes 3 2.5306 0.897291 0.0990832 FBgn0020440 87.94 P-phosphate metabolism 0.01 P-phosphorylation 6.81 F-protein kinase *F 279 P-M phase 1 2.52475 0.678774 0.0305492 FBgn0004374 35.11 P-DNA replication and chromosome cycle 0.13 P-cytoplasm organization and biogenesis 5.44 P-microtubule-based process *F 5840 C-ribosome 2 2.5221 0.719358 0.0391742 FBgn0039300 56.69 F-structural protein of ribosome/ribosomal protein 1.02 F-nucleic acid binding 0.99 C-cytosol *F 6950 P-stress response 3 2.51532 0.668152 0.0288403 FBgn0011274 226.85 P-response to external stimulus 0.16 P-defense response 0.5 P-signal transduction *F 15630 C-microtubule cytoskeleton 8 2.49769 0.676866 0.0310456 FBgn0013733 52.8 P-microtubule-based process 2.1 P-developmental processes 0.51 P-embryogenesis and morphogenesis *F 16070 P-RNA metabolism 4 2.4959 0.706374 0.0372392 FBgn0039558 43.73 C-spliceosome 1.22 C-nucleus 0.89 P-cytoplasm organization and biogenesis *F 4674 F-protein serine/threonine kinase 19 2.49549 0.745097 0.0465586 FBgn0033790 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 6396 P-RNA processing 4 2.49444 0.703037 0.0365595 FBgn0039558 44.35 C-spliceosome 1.22 C-nucleus 0.89 P-cytoplasm organization and biogenesis *F 4674 F-protein serine/threonine kinase 20 2.48685 0.743633 0.0465586 FBgn0003501 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 19226 P-transmission of nerve impulse 10 2.47963 0.657274 0.0279254 FBgn0001202 66.17 P-vesicle transport 0.83 P-intracellular protein traffic 1.13 P-protein transport *F 7268 P-synaptic transmission 10 2.47963 0.657274 0.0279254 FBgn0001202 66.17 P-vesicle transport 0.83 P-intracellular protein traffic 1.13 P-protein transport *F 4888 F-transmembrane receptor 2 2.47916 0.882472 0.0959401 FBgn0003218 2038.31 P-perception of external stimulus 0 P-perception of abiotic stimulus 0.06 P-sensory perception *F 7267 P-cell-cell signaling 10 2.47252 0.65752 0.0281838 FBgn0001202 65.54 P-vesicle transport 0.82 P-intracellular protein traffic 1.13 P-protein transport *F 5681 C-spliceosome 4 2.46884 0.646013 0.0262422 FBgn0035253 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.94 F-nucleic acid binding *F 7606 P-chemosensory perception 2 2.46572 0.644112 0.0260016 FBgn0003248 1127.9 F-G-protein coupled receptor/G-protein linked receptor 0.07 P-G-protein coupled receptor protein signaling pathway 1.74 F-receptor *F 7606 P-chemosensory perception 3 2.46572 0.644112 0.0260016 FBgn0003249 1127.9 F-G-protein coupled receptor/G-protein linked receptor 0.07 P-G-protein coupled receptor protein signaling pathway 1.74 F-receptor *F 7606 P-chemosensory perception 4 2.46572 0.644112 0.0260016 FBgn0003250 1127.9 F-G-protein coupled receptor/G-protein linked receptor 0.07 P-G-protein coupled receptor protein signaling pathway 1.74 F-receptor *F 5875 C-microtubule associated protein 10 2.46506 0.639863 0.025293 FBgn0034577 22.75 P-microtubule-based process 2.56 P-developmental processes 1.87 F-protein binding *F 5654 C-nucleoplasm 6 2.45384 0.714164 0.0406443 FBgn0003278 8.19 P-transcription 2.01 P-metabolism 1.85 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 280 P-nuclear division 2 2.45314 0.636534 0.0250611 FBgn0011674 34.64 P-DNA replication and chromosome cycle 0.12 P-cytoplasm organization and biogenesis 6.11 P-cell organization and biogenesis *F 67 P-DNA replication and chromosome cycle 1 2.45189 0.646007 0.0267301 FBgn0028581 8.81 P-nuclear division 3.06 P-DNA metabolism 2.03 P-M phase *F 5524 F-ATP binding 3 2.44918 0.828532 0.0753356 FBgn0025115 7.2 F-hydrolase, acting on acid anhydrides 7.2 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.93 F-hydrolase *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 3 2.44714 0.648021 0.027227 FBgn0004551 49.74 F-P-P-bond-hydrolyzis-driven transporter 0.47 C-cell 1.66 C-intracellular/protoplasm *F 9607 P-response to biotic stimulus 1 2.4406 0.709537 0.0400867 FBgn0023000 58.91 P-stress response 2.94 P-signal transduction 0.36 P-cell surface receptor linked signal transduction *F 4930 F-G-protein coupled receptor/G-protein linked receptor 2 2.43841 0.746994 0.0496592 FBgn0039747 23.23 P-G-protein coupled receptor protein signaling pathway 1.84 C-integral membrane protein 1.37 C-intracellular/protoplasm *F 4930 F-G-protein coupled receptor/G-protein linked receptor 3 2.43841 0.746994 0.0496592 FBgn0037546 23.23 P-G-protein coupled receptor protein signaling pathway 1.84 C-integral membrane protein 1.37 C-intracellular/protoplasm *F 16791 F-phosphoric monoester hydrolase 3 2.43696 0.719265 0.0425598 FBgn0013349 65.14 F-phosphatase 0.88 F-hydrolase, acting on acid anhydrides 0.88 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides *F 3735 F-structural protein of ribosome/ribosomal protein 4 2.43172 0.693608 0.0368129 FBgn0011285 63.32 C-ribosome 0.88 P-phosphate metabolism 1.09 P-phosphorylation *F 15630 C-microtubule cytoskeleton 9 2.40466 0.661309 0.0310456 FBgn0024923 61.91 P-microtubule-based process 0.34 P-gametogenesis 2.64 P-cell growth and/or maintenance *F 5654 C-nucleoplasm 7 2.39969 0.704907 0.0406443 FBgn0002542 10.26 P-transcription 2.37 P-metabolism 2.03 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 7600 P-sensory perception 2 2.39548 0.672668 0.0336512 FBgn0019940 81.18 P-perception of abiotic stimulus 3.59 P-G-protein coupled receptor protein signaling pathway 0.36 F-G-protein coupled receptor/G-protein linked receptor *F 5740 C-mitochondrial membrane 3 2.3952 0.700981 0.0399025 FBgn0017646 40.07 F-carrier/carrier type transporter 1.21 F-ATP binding 0.83 F-purine nucleotide binding *F 280 P-nuclear division 3 2.39204 0.62645 0.0250611 FBgn0000163 34.64 P-DNA replication and chromosome cycle 0.12 P-cytoplasm organization and biogenesis 6.11 P-cell organization and biogenesis *F 7397 P-histogenesis and organogenesis 3 2.38974 0.724516 0.0459198 FBgn0004839 71.73 P-phosphate metabolism 0.01 P-phosphorylation 15.52 P-cell communication *F 5740 C-mitochondrial membrane 4 2.3803 0.698416 0.0399025 FBgn0029963 40.07 F-carrier/carrier type transporter 1.12 P-intracellular protein traffic 0.89 P-protein transport *F 7606 P-chemosensory perception 5 2.3762 0.629281 0.0260016 FBgn0019940 1127.9 F-G-protein coupled receptor/G-protein linked receptor 0.07 P-G-protein coupled receptor protein signaling pathway 1.74 F-receptor *F 3723 F-RNA binding 2 2.37072 0.781487 0.0635331 FBgn0035838 1108.29 P-RNA splicing 0.01 P-mRNA splicing 2.38 P-mRNA processing *F 5681 C-spliceosome 5 2.36873 0.629409 0.0262422 FBgn0015317 13997.25 P-RNA splicing 0 P-mRNA splicing 0.57 F-RNA binding *F 5681 C-spliceosome 6 2.36396 0.628611 0.0262422 FBgn0035838 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.94 F-nucleic acid binding *F 4674 F-protein serine/threonine kinase 21 2.36055 0.721899 0.0465586 FBgn0040505 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 19226 P-transmission of nerve impulse 11 2.33479 0.633071 0.0279254 FBgn0028968 84.53 P-vesicle transport 0.82 P-intracellular protein traffic 0.83 C-cellular_component *F 7268 P-synaptic transmission 11 2.33479 0.633071 0.0279254 FBgn0028968 84.53 P-vesicle transport 0.82 P-intracellular protein traffic 0.83 C-cellular_component *F 7267 P-cell-cell signaling 11 2.33088 0.633809 0.0281838 FBgn0028968 83.67 P-vesicle transport 0.81 P-intracellular protein traffic 0.84 C-cellular_component *F 7345 P-embryogenesis and morphogenesis 3 2.32882 0.755202 0.057544 FBgn0020440 0 P-phosphorylation 282.54 P-phosphate metabolism 5 P-cell communication *F 4674 F-protein serine/threonine kinase 22 2.32395 0.715489 0.0465586 FBgn0011737 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 3735 F-structural protein of ribosome/ribosomal protein 5 2.32111 0.674618 0.0368129 FBgn0016760 45.61 C-ribosome 1.18 C-cytosolic ribosome 0.95 C-cytoplasm *F 3735 F-structural protein of ribosome/ribosomal protein 6 2.32111 0.674618 0.0368129 FBgn0000164 45.61 C-ribosome 1.18 C-cytosolic ribosome 0.95 C-cytoplasm *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 3 2.31836 0.861794 0.0988553 FBgn0004913 10.39 C-nucleoplasm 2.92 F-DNA binding 1.46 F-nucleic acid binding *F 7606 P-chemosensory perception 6 2.31616 0.619208 0.0260016 FBgn0014019 1127.9 F-G-protein coupled receptor/G-protein linked receptor 0.07 P-G-protein coupled receptor protein signaling pathway 1.74 F-receptor *F 6810 P-transport 2 2.31278 0.755846 0.0586138 FBgn0015624 7.05 P-neurotransmitter release 7.05 P-neurotransmitter maintenance 0.36 C-cell *F 4888 F-transmembrane receptor 3 2.31017 0.854116 0.0959401 FBgn0000037 5.57 P-G-protein coupled receptor protein signaling pathway 3.39 C-integral membrane protein 1.85 C-plasma membrane/cell membrane *F 9581 P-perception of external stimulus 1 2.30854 0.714827 0.0470977 FBgn0003036 120.04 P-response to abiotic stimulus 0.44 P-metabolism 0.82 C-intracellular/protoplasm *F 6468 P-protein amino acid phosphorylation 2 2.30574 0.735219 0.0528445 FBgn0011774 35.33 F-protein kinase 1.47 F-protein serine/threonine kinase 0.91 F-enzyme *F 6952 P-defense response 3 2.30392 0.668208 0.0360579 FBgn0000261 30.77 P-stress response 1.34 P-cell growth and/or maintenance 1.17 C-cytoplasm *F 4674 F-protein serine/threonine kinase 23 2.30179 0.711583 0.0465586 FBgn0025936 127.48 P-protein amino acid phosphorylation 0.19 F-transmembrane receptor 2.34 P-phosphate metabolism *F 4674 F-protein serine/threonine kinase 24 2.30102 0.711447 0.0465586 FBgn0032006 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 9593 P-perception of chemical substance 3 2.29559 0.615732 0.0260016 FBgn0000120 80.49 P-sensory perception 0.78 C-cell 0.78 C-plasma membrane/cell membrane *F 4674 F-protein serine/threonine kinase 25 2.28584 0.70876 0.0465586 FBgn0028484 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 7275 P-developmental processes 4 2.27409 0.854624 0.0990832 FBgn0021760 66.37 P-microtubule-based process 18.95 P-cell growth and/or maintenance 0.1 P-cell organization and biogenesis *F 15399 F-primary active transporter 1 2.25076 0.707024 0.0477529 FBgn0017646 28.51 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 1.53 F-ATPase 0.93 P-protein transport *F 16791 F-phosphoric monoester hydrolase 4 2.24816 0.686339 0.0425598 FBgn0027890 54.91 F-phosphatase 0.89 F-hydrolase, acting on acid anhydrides 0.89 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides *F 16021 C-integral membrane protein 1 2.23908 0.836834 0.0937562 FBgn0025680 0.07 P-perception of abiotic stimulus 10.87 F-G-protein coupled receptor/G-protein linked receptor 9.3 P-perception of external stimulus *F 7398 P-ectoderm development 3 2.23644 0.671302 0.0394457 FBgn0004839 74.87 P-phosphate metabolism 0.01 P-phosphorylation 15.96 P-cell communication *F 5681 C-spliceosome 7 2.23569 0.606894 0.0262422 FBgn0030631 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.41 F-RNA binding *F 5681 C-spliceosome 8 2.23412 0.606624 0.0262422 FBgn0029138 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.37 F-RNA binding *F 16302 F-phosphatase 1 2.22719 0.67668 0.041115 FBgn0028966 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 2 2.22719 0.67668 0.041115 FBgn0030465 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 3 2.22719 0.67668 0.041115 FBgn0034225 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 4 2.22719 0.67668 0.041115 FBgn0034226 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 5 2.22719 0.67668 0.041115 FBgn0033538 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 6 2.22719 0.67668 0.041115 FBgn0035195 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 7 2.22719 0.67668 0.041115 FBgn0030264 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 8 2.22719 0.67668 0.041115 FBgn0030265 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 16302 F-phosphatase 9 2.22719 0.67668 0.041115 FBgn0039698 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 1.01 F-ATPase *F 5681 C-spliceosome 9 2.22711 0.605423 0.0262422 FBgn0014189 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.41 F-RNA binding *F 5681 C-spliceosome 10 2.2269 0.605386 0.0262422 FBgn0033160 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.49 F-RNA binding *F 9593 P-perception of chemical substance 4 2.2228 0.60333 0.0260016 FBgn0002938 90.64 P-sensory perception 2.06 C-intracellular/protoplasm 0.56 C-cell *F 7345 P-embryogenesis and morphogenesis 4 2.22187 0.736053 0.057544 FBgn0004839 0 P-phosphorylation 282.54 P-phosphate metabolism 12.22 P-cell communication *F 279 P-M phase 2 2.22166 0.627417 0.0305492 FBgn0015270 387.33 P-DNA replication and chromosome cycle 0.22 C-nucleoplasm 0.45 P-DNA metabolism *F 4930 F-G-protein coupled receptor/G-protein linked receptor 4 2.21932 0.708406 0.0496592 FBgn0020277 8.29 P-perception of chemical substance 8.29 P-chemosensory perception 5.3 P-perception of abiotic stimulus *F 5681 C-spliceosome 11 2.21493 0.603332 0.0262422 FBgn0026003 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.54 F-RNA binding *F 7275 P-developmental processes 5 2.21215 0.843714 0.0990832 FBgn0003525 655.44 P-phosphate metabolism 0 P-dephosphorylation 18.95 P-cell growth and/or maintenance *F 16181 P-synaptic vesicle transport/synaptic vesicle recycling 2 2.21198 0.601474 0.0260016 FBgn0015624 7.91 P-neurotransmitter release 7.91 P-neurotransmitter maintenance 0.93 C-nucleus *F 4674 F-protein serine/threonine kinase 26 2.20446 0.694197 0.0465586 FBgn0004603 37.91 P-protein amino acid phosphorylation 2.96 P-response to abiotic stimulus 0.46 P-response to external stimulus *F 4674 F-protein serine/threonine kinase 27 2.20226 0.693802 0.0465586 FBgn0039015 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 28 2.20226 0.693802 0.0465586 FBgn0034020 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 29 2.20226 0.693802 0.0465586 FBgn0038534 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 30 2.20226 0.693802 0.0465586 FBgn0032011 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 31 2.20226 0.693802 0.0465586 FBgn0038630 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 32 2.20226 0.693802 0.0465586 FBgn0037925 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 33 2.20226 0.693802 0.0465586 FBgn0030018 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 34 2.20226 0.693802 0.0465586 FBgn0031518 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 35 2.20226 0.693802 0.0465586 FBgn0040298 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 36 2.20226 0.693802 0.0465586 FBgn0038167 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4674 F-protein serine/threonine kinase 37 2.20226 0.693802 0.0465586 FBgn0037491 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 4930 F-G-protein coupled receptor/G-protein linked receptor 5 2.19551 0.704095 0.0496592 FBgn0004435 172.55 P-G-protein coupled receptor protein signaling pathway 8.31 P-perception of abiotic stimulus 0.23 C-intracellular/protoplasm *F 16302 F-phosphatase 10 2.18549 0.669212 0.041115 FBgn0030916 39.41 F-phosphoric monoester hydrolase 1.1 F-hydrolase, acting on ester bonds/esterase 0.97 C-cytosol *F 4930 F-G-protein coupled receptor/G-protein linked receptor 6 2.17417 0.700212 0.0496592 FBgn0003218 15.76 P-perception of abiotic stimulus 0.11 P-sensory perception 6.19 P-perception of chemical substance *F 5681 C-spliceosome 12 2.16023 0.593885 0.0262422 FBgn0022943 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.41 F-RNA binding *F 5681 C-spliceosome 13 2.16002 0.593848 0.0262422 FBgn0038887 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.49 F-RNA binding *F 5681 C-spliceosome 14 2.16002 0.593848 0.0262422 FBgn0022942 9771.35 P-RNA splicing 0.01 P-mRNA splicing 0.49 F-RNA binding *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 4 2.14254 0.59621 0.027227 FBgn0032501 33.35 F-P-P-bond-hydrolyzis-driven transporter 1.21 C-cell 1.21 F-carrier/carrier type transporter *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 5 2.14254 0.59621 0.027227 FBgn0027582 33.35 F-P-P-bond-hydrolyzis-driven transporter 1.21 C-cell 1.21 F-carrier/carrier type transporter *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 6 2.14254 0.59621 0.027227 FBgn0037989 33.35 F-P-P-bond-hydrolyzis-driven transporter 1.21 C-cell 1.21 F-carrier/carrier type transporter *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 7 2.14254 0.59621 0.027227 FBgn0030746 33.35 F-P-P-bond-hydrolyzis-driven transporter 1.21 C-cell 1.21 F-carrier/carrier type transporter *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 8 2.14254 0.59621 0.027227 FBgn0033837 33.35 F-P-P-bond-hydrolyzis-driven transporter 1.21 C-cell 1.21 F-carrier/carrier type transporter *F 5654 C-nucleoplasm 8 2.13339 0.657846 0.0406443 FBgn0004406 3.88 P-cell cycle/cell-division cycle 3.08 P-DNA replication and chromosome cycle 2.02 F-transferase, transferring phosphorus-containing groups *F 5875 C-microtubule associated protein 11 2.12839 0.583021 0.025293 FBgn0026193 67.08 P-microtubule-based process 0.04 P-phosphate metabolism 25.68 P-phosphorylation *F 5743 C-mitochondrial inner membrane/cristae 3 2.12781 0.592278 0.0269774 FBgn0037710 47.33 P-protein transport 1.29 P-metabolism 0.79 P-organelle organization and biogenesis *F 16192 P-vesicle transport 2 2.11913 0.635478 0.0360579 FBgn0015624 7.71 P-neurotransmitter release 7.71 P-neurotransmitter maintenance 0.5 C-cell *F 6952 P-defense response 4 2.11229 0.634243 0.0360579 FBgn0002901 63.19 P-stress response 0.28 P-cell cycle/cell-division cycle 2.14 P-M phase *F 6952 P-defense response 5 2.11152 0.634105 0.0360579 FBgn0011706 30.77 P-stress response 1.17 C-cytoplasm 1.16 P-cell growth and/or maintenance *F 16787 F-hydrolase 3 2.10684 0.552503 0.0208449 FBgn0030370 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 4 2.10684 0.552503 0.0208449 FBgn0032535 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 5 2.10684 0.552503 0.0208449 FBgn0039049 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 6 2.10684 0.552503 0.0208449 FBgn0039050 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 7 2.10684 0.552503 0.0208449 FBgn0039051 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 8 2.10684 0.552503 0.0208449 FBgn0039052 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 9 2.10684 0.552503 0.0208449 FBgn0039053 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 10 2.10684 0.552503 0.0208449 FBgn0034088 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 11 2.10684 0.552503 0.0208449 FBgn0033366 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 12 2.10684 0.552503 0.0208449 FBgn0029805 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 13 2.10684 0.552503 0.0208449 FBgn0031342 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 14 2.10684 0.552503 0.0208449 FBgn0035781 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 15 2.10684 0.552503 0.0208449 FBgn0037396 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 16 2.10684 0.552503 0.0208449 FBgn0038899 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 17 2.10684 0.552503 0.0208449 FBgn0033874 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 16787 F-hydrolase 18 2.10684 0.552503 0.0208449 FBgn0030223 0.62 P-phosphate metabolism 1.4 P-phosphorylation 0.87 F-purine nucleotide binding *F 67 P-DNA replication and chromosome cycle 2 2.09873 0.585828 0.0267301 FBgn0011205 16.12 P-nuclear division 2.81 P-M phase 1.2 P-phosphate metabolism *F 9628 P-response to abiotic stimulus 1 2.09593 0.707691 0.0559758 FBgn0033327 27.68 P-perception of external stimulus 4.07 P-stress response 0.29 P-response to biotic stimulus *F 9056 P-catabolism 1 2.09213 0.584667 0.0267301 FBgn0000986 1138.89 P-protein metabolism and modification 0.09 P-transport 0.58 C-cytoplasm *F 15630 C-microtubule cytoskeleton 10 2.08575 0.605896 0.0310456 FBgn0028388 61.91 P-microtubule-based process 5.64 P-developmental processes 0.25 P-gametogenesis *F 4674 F-protein serine/threonine kinase 38 2.085 0.672341 0.0465586 FBgn0037218 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 5856 C-cytoskeleton 8 2.08302 0.670359 0.0461318 FBgn0000275 13.86 P-microtubule-based process 3.06 P-cytoskeleton organization and biogenesis 0.41 P-gametogenesis *F 280 P-nuclear division 4 2.07709 0.572751 0.0250611 FBgn0004374 34.64 P-DNA replication and chromosome cycle 0.12 P-cytoplasm organization and biogenesis 6.11 P-cell organization and biogenesis *F 5198 F-structural protein 1 2.06123 0.783445 0.084918 FBgn0015806 32.61 C-ribosome 0.13 P-phosphorylation 7.3 P-phosphate metabolism *F 5525 F-GTP binding 7 2.05218 0.588761 0.0288403 FBgn0013349 8.44 F-hydrolase, acting on acid anhydrides 8.44 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.52 F-phosphatase *F 19001 F-guanyl nucleotide binding 7 2.04916 0.589592 0.0291072 FBgn0013349 8.4 F-hydrolase, acting on acid anhydrides 8.4 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.52 F-phosphatase *F 4674 F-protein serine/threonine kinase 39 2.04302 0.664519 0.0465586 FBgn0000017 37.91 P-protein amino acid phosphorylation 1.89 C-cellular_component 1.84 P-phosphate metabolism *F 6886 P-intracellular protein traffic 2 2.03996 0.629281 0.0379315 FBgn0015624 7.9 P-neurotransmitter release 7.9 P-neurotransmitter maintenance 0.51 C-cell *F 5886 C-plasma membrane/cell membrane 1 2.03827 0.809835 0.0981748 FBgn0003425 10.04 P-cell communication 4.57 P-embryogenesis and morphogenesis 0.6 P-ectoderm development *F 5856 C-cytoskeleton 9 2.0348 0.661371 0.0461318 FBgn0026193 0.01 P-phosphate metabolism 100.2 P-phosphorylation 30.03 P-microtubule-based process *F 6952 P-defense response 6 2.03108 0.619447 0.0360579 FBgn0041204 30.77 P-stress response 1.29 F-protein serine/threonine kinase 0.81 F-protein kinase *F 6468 P-protein amino acid phosphorylation 3 2.02878 0.684455 0.0528445 FBgn0003502 45.03 F-protein kinase 0.82 P-developmental processes 1.14 P-embryogenesis and morphogenesis *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 4 2.02705 0.809199 0.0988553 FBgn0036548 22.7 C-spliceosome 1.42 F-nucleic acid binding 0.77 C-nucleus *F 15630 C-microtubule cytoskeleton 11 2.01295 0.592767 0.0310456 FBgn0023177 208.25 P-microtubule-based process 0.02 P-phosphate metabolism 36.43 P-dephosphorylation *F 15630 C-microtubule cytoskeleton 12 2.0071 0.591703 0.0310456 FBgn0000183 61.91 P-microtubule-based process 0.19 P-gametogenesis 4.11 P-developmental processes *F 4674 F-protein serine/threonine kinase 40 1.99427 0.655344 0.0465586 FBgn0003733 37.91 P-protein amino acid phosphorylation 2.53 P-response to abiotic stimulus 0.53 P-response to external stimulus *F 67 P-DNA replication and chromosome cycle 3 1.98937 0.566399 0.0267301 FBgn0002716 8.14 P-nuclear division 2.93 P-DNA metabolism 1.98 P-M phase *F 280 P-nuclear division 5 1.98311 0.556134 0.0250611 FBgn0015270 378.58 P-DNA replication and chromosome cycle 0.23 C-nucleoplasm 0.41 P-DNA metabolism *F 9593 P-perception of chemical substance 5 1.98249 0.561221 0.0260016 FBgn0000253 52.67 P-sensory perception 1.37 C-intracellular/protoplasm 0.78 C-cell *F 15405 F-P-P-bond-hydrolyzis-driven transporter 2 1.98024 0.604584 0.0348337 FBgn0037710 46.09 P-protein transport 1.12 P-intracellular protein traffic 1.11 C-intracellular/protoplasm *F 19226 P-transmission of nerve impulse 12 1.9741 0.569972 0.0279254 FBgn0000257 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 12 1.9741 0.569972 0.0279254 FBgn0000257 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 6950 P-stress response 4 1.9729 0.574473 0.0288403 FBgn0003036 220.36 P-response to external stimulus 0.33 P-response to abiotic stimulus 0.46 P-metabolism *F 7275 P-developmental processes 6 1.97027 0.798826 0.0990832 FBgn0003391 41.46 P-cell communication 0.32 C-integral plasma membrane protein 3 C-plasma membrane/cell membrane *F 16887 F-ATPase 1 1.96527 0.739003 0.074817 FBgn0035473 25.23 F-P-P-bond-hydrolyzis-driven transporter 1.16 C-cytoplasm 1.14 C-mitochondrial membrane *F 5654 C-nucleoplasm 9 1.96421 0.626545 0.0406443 FBgn0019950 6.47 P-transcription 1.95 F-RNA polymerase II transcription factor 1.69 P-metabolism *F 7267 P-cell-cell signaling 12 1.961 0.56894 0.0281838 FBgn0000257 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 4888 F-transmembrane receptor 4 1.94863 0.787662 0.0959401 FBgn0037546 5.57 P-G-protein coupled receptor protein signaling pathway 2.68 C-integral membrane protein 1.51 P-cell communication *F 9582 P-perception of abiotic stimulus 1 1.94199 0.642996 0.0459198 FBgn0023423 55.03 P-cell surface receptor linked signal transduction 0.69 P-signal transduction 0.85 P-catabolism *F 5198 F-structural protein 2 1.93749 0.759584 0.084918 FBgn0035422 15.29 C-ribosome 1.62 P-protein biosynthesis/translation 0.89 P-phosphate metabolism *F 5198 F-structural protein 3 1.93749 0.759584 0.084918 FBgn0032987 15.29 C-ribosome 1.62 P-protein biosynthesis/translation 0.89 P-phosphate metabolism *F 15630 C-microtubule cytoskeleton 13 1.93246 0.578029 0.0310456 FBgn0000275 61.91 P-microtubule-based process 0.34 P-gametogenesis 2.19 P-developmental processes *F 16788 F-hydrolase, acting on ester bonds/esterase 1 1.93106 0.764601 0.0874984 FBgn0013349 27.89 F-phosphatase 0.81 P-phosphate metabolism 1.16 P-phosphorylation *F 15031 P-protein transport 2 1.92793 0.620444 0.0408319 FBgn0015624 7.82 P-neurotransmitter release 7.82 P-neurotransmitter maintenance 0.5 C-cell *F 3735 F-structural protein of ribosome/ribosomal protein 7 1.92007 0.602112 0.0368129 FBgn0036853 33.23 C-ribosome 1.17 C-cytosolic ribosome 0.95 C-cytoplasm *F 3735 F-structural protein of ribosome/ribosomal protein 8 1.92007 0.602112 0.0368129 FBgn0029973 33.23 C-ribosome 1.17 C-cytosolic ribosome 0.95 C-cytoplasm *F 7606 P-chemosensory perception 7 1.91939 0.54985 0.0260016 FBgn0003444 59.74 F-G-protein coupled receptor/G-protein linked receptor 1.53 F-receptor 1.43 C-membrane *F 4674 F-protein serine/threonine kinase 41 1.91792 0.640763 0.0465586 FBgn0000723 37.91 P-protein amino acid phosphorylation 1.84 P-phosphate metabolism 0.58 P-phosphorylation *F 7267 P-cell-cell signaling 13 1.91493 0.560537 0.0281838 FBgn0044819 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 4888 F-transmembrane receptor 5 1.905 0.779094 0.0959401 FBgn0000017 11.25 F-protein kinase 0.27 C-intracellular/protoplasm 3.4 C-cell *F 16817 F-hydrolase, acting on acid anhydrides 1 1.9033 0.785236 0.0988553 FBgn0010339 27.26 F-GTP binding 3.49 F-purine nucleotide binding 0.31 F-guanyl nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 1 1.90325 0.785225 0.0988553 FBgn0010339 27.27 F-GTP binding 3.49 F-purine nucleotide binding 0.31 F-guanyl nucleotide binding *F 19226 P-transmission of nerve impulse 13 1.90213 0.556863 0.0279254 FBgn0044819 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 13 1.90213 0.556863 0.0279254 FBgn0044819 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 14 1.89943 0.556366 0.0279254 FBgn0000482 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 14 1.89943 0.556366 0.0279254 FBgn0000482 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 9581 P-perception of external stimulus 2 1.89847 0.639003 0.0470977 FBgn0005614 35.96 P-response to abiotic stimulus 0.87 P-transport 0.87 C-intracellular/protoplasm *F 7267 P-cell-cell signaling 14 1.8966 0.557174 0.0281838 FBgn0000482 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 16817 F-hydrolase, acting on acid anhydrides 2 1.89627 0.783845 0.0988553 FBgn0037843 11.27 F-ATP binding 2.4 F-purine nucleotide binding 0.95 F-GTP binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 2 1.89596 0.783783 0.0988553 FBgn0037843 11.27 F-ATP binding 2.4 F-purine nucleotide binding 0.95 F-GTP binding *F 279 P-M phase 3 1.88786 0.567359 0.0305492 FBgn0002924 25.91 P-DNA replication and chromosome cycle 0.13 P-cytoplasm organization and biogenesis 5.44 P-microtubule-based process *F 5739 C-mitochondrion 1 1.87286 0.751589 0.086896 FBgn0002583 12 C-ribosome 2.01 F-structural protein of ribosome/ribosomal protein 0.8 C-membrane *F 7275 P-developmental processes 7 1.86795 0.778708 0.0990832 FBgn0004839 87.94 P-phosphate metabolism 0.01 P-phosphorylation 7.69 P-cell communication *F 5198 F-structural protein 4 1.86585 0.745353 0.084918 FBgn0011285 25.26 C-ribosome 15.82 P-phosphate metabolism 0.07 P-phosphorylation *F 67 P-DNA replication and chromosome cycle 4 1.86503 0.543815 0.0267301 FBgn0000404 16.12 P-nuclear division 2.5 P-M phase 1.2 P-phosphate metabolism *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 5 1.86386 0.777388 0.0988553 FBgn0035843 22.7 C-spliceosome 0.77 C-nucleus 1.25 F-nucleic acid binding *F 19226 P-transmission of nerve impulse 15 1.85865 0.548851 0.0279254 FBgn0035602 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 15 1.85865 0.548851 0.0279254 FBgn0035602 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 4930 F-G-protein coupled receptor/G-protein linked receptor 7 1.84874 0.638549 0.0496592 FBgn0000037 23.23 P-G-protein coupled receptor protein signaling pathway 2.13 C-integral membrane protein 1.99 C-cell *F 6952 P-defense response 7 1.84684 0.58494 0.0360579 FBgn0004367 85.14 P-stress response 0.1 P-phosphate metabolism 7.59 P-phosphorylation *F 7267 P-cell-cell signaling 15 1.84353 0.547366 0.0281838 FBgn0035602 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 6 1.84218 0.773029 0.0988553 FBgn0036683 22.7 C-spliceosome 0.77 C-nucleus 1.25 F-nucleic acid binding *F 19226 P-transmission of nerve impulse 16 1.84216 0.545795 0.0279254 FBgn0045026 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 17 1.84216 0.545795 0.0279254 FBgn0045027 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 18 1.84216 0.545795 0.0279254 FBgn0044804 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 19 1.84216 0.545795 0.0279254 FBgn0044805 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 20 1.84216 0.545795 0.0279254 FBgn0044806 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 21 1.84216 0.545795 0.0279254 FBgn0044807 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 16 1.84216 0.545795 0.0279254 FBgn0045026 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 17 1.84216 0.545795 0.0279254 FBgn0045027 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 18 1.84216 0.545795 0.0279254 FBgn0044804 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 19 1.84216 0.545795 0.0279254 FBgn0044805 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 20 1.84216 0.545795 0.0279254 FBgn0044806 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 21 1.84216 0.545795 0.0279254 FBgn0044807 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7010 P-cytoskeleton organization and biogenesis 1 1.84138 0.63026 0.0477529 FBgn0038565 4.87 C-cytoskeleton 4.19 C-microtubule cytoskeleton 1.61 F-structural protein *F 19226 P-transmission of nerve impulse 22 1.83601 0.544652 0.0279254 FBgn0037357 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 22 1.83601 0.544652 0.0279254 FBgn0037357 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7267 P-cell-cell signaling 16 1.82978 0.544807 0.0281838 FBgn0045026 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 17 1.82978 0.544807 0.0281838 FBgn0045027 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 18 1.82978 0.544807 0.0281838 FBgn0044804 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 19 1.82978 0.544807 0.0281838 FBgn0044805 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 20 1.82978 0.544807 0.0281838 FBgn0044806 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 21 1.82978 0.544807 0.0281838 FBgn0044807 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 5198 F-structural protein 5 1.82683 0.737469 0.084918 FBgn0016760 19.57 C-ribosome 1.2 C-cell 0.88 C-cellular_component *F 5198 F-structural protein 6 1.82683 0.737469 0.084918 FBgn0000164 19.57 C-ribosome 1.2 C-cell 0.88 C-cellular_component *F 7267 P-cell-cell signaling 22 1.82522 0.543956 0.0281838 FBgn0037357 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 19226 P-transmission of nerve impulse 23 1.82457 0.542524 0.0279254 FBgn0044871 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 24 1.82457 0.542524 0.0279254 FBgn0037789 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 25 1.82457 0.542524 0.0279254 FBgn0045028 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 26 1.82457 0.542524 0.0279254 FBgn0044509 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 27 1.82457 0.542524 0.0279254 FBgn0044803 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 28 1.82457 0.542524 0.0279254 FBgn0044818 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 23 1.82457 0.542524 0.0279254 FBgn0044871 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 24 1.82457 0.542524 0.0279254 FBgn0037789 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 25 1.82457 0.542524 0.0279254 FBgn0045028 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 26 1.82457 0.542524 0.0279254 FBgn0044509 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 27 1.82457 0.542524 0.0279254 FBgn0044803 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 28 1.82457 0.542524 0.0279254 FBgn0044818 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 29 1.82373 0.542366 0.0279254 FBgn0024509 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 30 1.82373 0.542366 0.0279254 FBgn0034708 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 29 1.82373 0.542366 0.0279254 FBgn0024509 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 30 1.82373 0.542366 0.0279254 FBgn0034708 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7267 P-cell-cell signaling 23 1.81965 0.542918 0.0281838 FBgn0024509 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 24 1.81965 0.542918 0.0281838 FBgn0034708 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 16788 F-hydrolase, acting on ester bonds/esterase 2 1.81929 0.742221 0.0874984 FBgn0027890 24.34 F-phosphatase 0.81 P-phosphate metabolism 1.16 P-phosphorylation *F 67 P-DNA replication and chromosome cycle 5 1.81744 0.535026 0.0267301 FBgn0000405 16.12 P-nuclear division 2.5 P-M phase 1.2 P-phosphate metabolism *F 9628 P-response to abiotic stimulus 2 1.81387 0.653107 0.0559758 FBgn0030310 27.68 P-perception of external stimulus 0.21 P-response to biotic stimulus 4.07 P-stress response *F 5740 C-mitochondrial membrane 5 1.81258 0.594494 0.0399025 FBgn0027786 25.54 F-carrier/carrier type transporter 1.12 P-intracellular protein traffic 0.89 P-protein transport *F 7267 P-cell-cell signaling 25 1.81235 0.541554 0.0281838 FBgn0044871 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 26 1.81235 0.541554 0.0281838 FBgn0037789 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 27 1.81235 0.541554 0.0281838 FBgn0045028 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 28 1.81235 0.541554 0.0281838 FBgn0044509 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 29 1.81235 0.541554 0.0281838 FBgn0044803 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 30 1.81235 0.541554 0.0281838 FBgn0044818 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 19226 P-transmission of nerve impulse 31 1.81137 0.54006 0.0279254 FBgn0015816 42.91 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 7268 P-synaptic transmission 31 1.81137 0.54006 0.0279254 FBgn0015816 42.91 P-vesicle transport 0.8 P-intracellular protein traffic 1.15 P-protein transport *F 6952 P-defense response 8 1.80834 0.577555 0.0360579 FBgn0023000 30.77 P-stress response 3.02 P-signal transduction 0.38 P-cell surface receptor linked signal transduction *F 19226 P-transmission of nerve impulse 32 1.80816 0.53946 0.0279254 FBgn0039262 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 33 1.80816 0.53946 0.0279254 FBgn0039303 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 19226 P-transmission of nerve impulse 34 1.80816 0.53946 0.0279254 FBgn0039335 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 32 1.80816 0.53946 0.0279254 FBgn0039262 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 33 1.80816 0.53946 0.0279254 FBgn0039303 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7268 P-synaptic transmission 34 1.80816 0.53946 0.0279254 FBgn0039335 42.91 P-vesicle transport 0.84 P-intracellular protein traffic 1.11 P-protein transport *F 7275 P-developmental processes 8 1.80666 0.766323 0.0990832 FBgn0000258 3750.15 P-phosphate metabolism 0 P-phosphorylation 9.11 F-protein kinase *F 16817 F-hydrolase, acting on acid anhydrides 3 1.80449 0.765377 0.0988553 FBgn0015788 24.68 F-GTP binding 3.32 F-purine nucleotide binding 0.32 F-guanyl nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 4 1.80449 0.765377 0.0988553 FBgn0015793 24.68 F-GTP binding 3.32 F-purine nucleotide binding 0.32 F-guanyl nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 5 1.80449 0.765377 0.0988553 FBgn0015794 24.68 F-GTP binding 3.32 F-purine nucleotide binding 0.32 F-guanyl nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 3 1.80444 0.765368 0.0988553 FBgn0015788 24.68 F-GTP binding 3.32 F-purine nucleotide binding 0.32 F-guanyl nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 4 1.80444 0.765368 0.0988553 FBgn0015793 24.68 F-GTP binding 3.32 F-purine nucleotide binding 0.32 F-guanyl nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 5 1.80444 0.765368 0.0988553 FBgn0015794 24.68 F-GTP binding 3.32 F-purine nucleotide binding 0.32 F-guanyl nucleotide binding *F 7267 P-cell-cell signaling 31 1.79866 0.53899 0.0281838 FBgn0039262 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 32 1.79866 0.53899 0.0281838 FBgn0039303 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 33 1.79866 0.53899 0.0281838 FBgn0039335 42.54 P-vesicle transport 0.84 P-intracellular protein traffic 1.12 P-protein transport *F 7267 P-cell-cell signaling 34 1.79799 0.538865 0.0281838 FBgn0015816 42.54 P-vesicle transport 0.8 P-intracellular protein traffic 1.16 P-protein transport *F 5525 F-GTP binding 8 1.79659 0.541889 0.0288403 FBgn0027890 7.54 F-hydrolase, acting on acid anhydrides 7.54 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.53 F-phosphatase *F 3702 F-RNA polymerase II transcription factor 1 1.79648 0.688836 0.0688652 FBgn0019949 0.09 F-transferase, transferring phosphorus-containing groups 7.77 C-nucleus 6.19 F-kinase *F 19001 F-guanyl nucleotide binding 8 1.79476 0.542867 0.0291072 FBgn0027890 7.51 F-hydrolase, acting on acid anhydrides 7.51 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.53 F-phosphatase *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 9 1.79312 0.533076 0.027227 FBgn0034732 33.35 F-P-P-bond-hydrolyzis-driven transporter 0.54 C-integral plasma membrane protein 1.53 C-integral membrane protein *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 10 1.79312 0.533076 0.027227 FBgn0038691 33.35 F-P-P-bond-hydrolyzis-driven transporter 0.54 C-integral plasma membrane protein 1.53 C-integral membrane protein *F 16773 F-phosphotransferase, alcohol group as acceptor 3 1.79016 0.72279 0.0820352 FBgn0039790 23.29 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 4 1.79016 0.72279 0.0820352 FBgn0039958 23.29 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 5 1.79016 0.72279 0.0820352 FBgn0031451 23.29 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 6 1.79016 0.72279 0.0820352 FBgn0037315 23.29 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 7 1.79016 0.72279 0.0820352 FBgn0035957 23.29 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 8 1.79016 0.72279 0.0820352 FBgn0036030 23.29 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 9628 P-response to abiotic stimulus 3 1.78326 0.646947 0.0559758 FBgn0037906 27.68 P-perception of external stimulus 0.21 P-response to biotic stimulus 4.07 P-stress response *F 7017 P-microtubule-based process 1 1.78138 0.557574 0.0327341 FBgn0001313 15.6 C-microtubule cytoskeleton 1.81 C-microtubule associated protein 0.8 P-phosphate metabolism *F 7017 P-microtubule-based process 2 1.78138 0.557574 0.0327341 FBgn0039246 15.6 C-microtubule cytoskeleton 1.81 C-microtubule associated protein 0.8 P-phosphate metabolism *F 4674 F-protein serine/threonine kinase 42 1.77874 0.613507 0.0465586 FBgn0031793 37.91 P-protein amino acid phosphorylation 0.54 F-transmembrane receptor 1.84 P-phosphate metabolism *F 4674 F-protein serine/threonine kinase 43 1.77874 0.613507 0.0465586 FBgn0031797 37.91 P-protein amino acid phosphorylation 0.54 F-transmembrane receptor 1.84 P-phosphate metabolism *F 3702 F-RNA polymerase II transcription factor 2 1.77254 0.683937 0.0688652 FBgn0039218 10.93 C-nucleus 0.3 F-transferase, transferring phosphorus-containing groups 2.3 P-transcription *F 5875 C-microtubule associated protein 12 1.77241 0.519044 0.025293 FBgn0024180 67.08 P-microtubule-based process 0.39 P-M phase 1.71 P-nuclear division *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 7 1.77189 0.75868 0.0988553 FBgn0039558 22.7 C-spliceosome 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 5654 C-nucleoplasm 10 1.77151 0.589448 0.0406443 FBgn0028996 6.04 P-transcription 1.89 F-RNA polymerase II transcription factor 1.64 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 9 1.75828 0.716208 0.0820352 FBgn0034356 23.29 F-kinase 0.97 F-lyase 0.98 P-phosphorylation *F 6468 P-protein amino acid phosphorylation 4 1.75652 0.631152 0.0528445 FBgn0000275 21.95 F-protein kinase 1.4 F-protein serine/threonine kinase 1.19 P-microtubule-based process *F 6259 P-DNA metabolism 1 1.75453 0.545595 0.0312608 FBgn0004374 3.54 F-DNA binding 3.33 P-cell cycle/cell-division cycle 3.2 P-DNA replication and chromosome cycle *F 9628 P-response to abiotic stimulus 4 1.75389 0.64099 0.0559758 FBgn0035976 27.68 P-perception of external stimulus 0.21 P-response to biotic stimulus 4.07 P-stress response *F 7166 P-cell surface receptor linked signal transduction 2 1.75137 0.623585 0.0509331 FBgn0002936 121.6 P-perception of abiotic stimulus 5.43 C-cell 0.27 P-perception of external stimulus *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 8 1.74937 0.75401 0.0988553 FBgn0027894 9.83 C-nucleoplasm 1.99 F-DNA binding 0.77 C-nucleus *F 9628 P-response to abiotic stimulus 5 1.74822 0.639835 0.0559758 FBgn0035975 27.68 P-perception of external stimulus 0.21 P-response to biotic stimulus 4.07 P-stress response *F 16181 P-synaptic vesicle transport/synaptic vesicle recycling 3 1.73658 0.516107 0.0260016 FBgn0013762 6.18 P-neurotransmitter release 6.18 P-neurotransmitter maintenance 0.9 P-phosphate metabolism *F 3824 F-enzyme 19 1.73004 0.667127 0.0660693 FBgn0020414 0.45 F-signal transducer 0.58 C-extracellular 0.59 P-phosphate metabolism *F 3824 F-enzyme 20 1.73004 0.667127 0.0660693 FBgn0020415 0.45 F-signal transducer 0.58 C-extracellular 0.59 P-phosphate metabolism *F 3824 F-enzyme 21 1.73004 0.667127 0.0660693 FBgn0020416 0.45 F-signal transducer 0.58 C-extracellular 0.59 P-phosphate metabolism *F 4674 F-protein serine/threonine kinase 44 1.72919 0.603583 0.0465586 FBgn0003731 37.91 P-protein amino acid phosphorylation 3.48 P-response to abiotic stimulus 0.37 F-transmembrane receptor *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 11 1.72834 0.520887 0.027227 FBgn0002921 33.35 F-P-P-bond-hydrolyzis-driven transporter 0.54 C-integral plasma membrane protein 0.63 C-cell *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 9 1.71994 0.747855 0.0988553 FBgn0029094 18.68 C-nucleoplasm 1.6 C-intracellular/protoplasm 0.66 C-nucleus *F 3735 F-structural protein of ribosome/ribosomal protein 9 1.71983 0.563544 0.0368129 FBgn0039857 33.23 C-ribosome 0.95 C-cytoplasm 1.05 F-nucleic acid binding *F 16820 F-hydrolase, acting on acid anhydrides\, - catalysing transmembrane movement of substances 12 1.71075 0.517551 0.027227 FBgn0035473 33.35 F-P-P-bond-hydrolyzis-driven transporter 0.5 C-cell 1.87 C-intracellular/protoplasm *F 16773 F-phosphotransferase, alcohol group as acceptor 10 1.70996 0.706107 0.0820352 FBgn0003067 23.29 F-kinase 0.97 F-lyase 0.97 P-metabolism *F 4888 F-transmembrane receptor 6 1.70853 0.738938 0.0959401 FBgn0002936 345.1 P-perception of external stimulus 0.01 P-perception of abiotic stimulus 3.57 C-integral membrane protein *F 15290 F-electrochemical potential-driven transporter 1 1.69464 0.576786 0.0413048 FBgn0038309 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15290 F-electrochemical potential-driven transporter 2 1.69464 0.576786 0.0413048 FBgn0038407 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15290 F-electrochemical potential-driven transporter 3 1.69464 0.576786 0.0413048 FBgn0039176 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15290 F-electrochemical potential-driven transporter 4 1.69464 0.576786 0.0413048 FBgn0034956 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15290 F-electrochemical potential-driven transporter 5 1.69464 0.576786 0.0413048 FBgn0038719 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15291 F-porter 1 1.69464 0.576786 0.0413048 FBgn0038309 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15291 F-porter 2 1.69464 0.576786 0.0413048 FBgn0038407 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15291 F-porter 3 1.69464 0.576786 0.0413048 FBgn0039176 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15291 F-porter 4 1.69464 0.576786 0.0413048 FBgn0034956 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 15291 F-porter 5 1.69464 0.576786 0.0413048 FBgn0038719 5.55 F-cation transporter 3.9 F-ion transporter 0.9 P-phosphate metabolism *F 9581 P-perception of external stimulus 3 1.69458 0.598531 0.0470977 FBgn0003888 35.96 P-response to abiotic stimulus 0.76 C-intracellular/protoplasm 0.79 C-microtubule cytoskeleton *F 16773 F-phosphotransferase, alcohol group as acceptor 11 1.69401 0.70274 0.0820352 FBgn0037146 23.29 F-kinase 0.96 P-metabolism 0.97 P-biosynthesis/anabolism *F 7397 P-histogenesis and organogenesis 4 1.69228 0.593782 0.0459198 FBgn0000634 15.52 P-cell communication 1.77 C-plasma membrane/cell membrane 0.77 P-phosphate metabolism *F 5515 F-protein binding 1 1.69061 0.690248 0.0774462 FBgn0025865 3.59 P-cytoskeleton organization and biogenesis 2.35 C-cytoskeleton 2.24 P-cell organization and biogenesis *F 6996 P-organelle organization and biogenesis 1 1.68729 0.631917 0.0574116 FBgn0038565 4.96 C-cytoskeleton 3.48 C-microtubule cytoskeleton 1.55 F-structural protein *F 5515 F-protein binding 2 1.68629 0.689334 0.0774462 FBgn0010349 5.52 P-cytoskeleton organization and biogenesis 3.55 C-cytoskeleton 3 P-cell organization and biogenesis *F 9605 P-response to external stimulus 4 1.68531 0.729048 0.0937562 FBgn0023423 7.05 P-cell surface receptor linked signal transduction 4.11 P-signal transduction 0.55 P-protein metabolism and modification *F 5875 C-microtubule associated protein 13 1.68448 0.502548 0.025293 FBgn0040207 22.75 P-microtubule-based process 1.67 F-protein binding 0.81 P-organelle organization and biogenesis *F 5875 C-microtubule associated protein 14 1.68448 0.502548 0.025293 FBgn0040208 22.75 P-microtubule-based process 1.67 F-protein binding 0.81 P-organelle organization and biogenesis *F 67 P-DNA replication and chromosome cycle 6 1.68131 0.509411 0.0267301 FBgn0002673 0 P-phosphate metabolism 192.45 P-dephosphorylation 41.75 P-nuclear division *F 7017 P-microtubule-based process 3 1.68093 0.538116 0.0327341 FBgn0038565 27.65 C-microtubule cytoskeleton 0.8 P-phosphate metabolism 1.24 C-cytoskeleton *F 67 P-DNA replication and chromosome cycle 7 1.6805 0.509258 0.0267301 FBgn0003124 21.24 P-phosphorylation 0.05 P-phosphate metabolism 16.12 P-nuclear division *F 7345 P-embryogenesis and morphogenesis 5 1.67453 0.629683 0.057544 FBgn0013984 0 P-phosphorylation 282.54 P-phosphate metabolism 26.57 P-cell communication *F 7028 P-cytoplasm organization and biogenesis 1 1.67353 0.637094 0.0599791 FBgn0038565 4.79 C-cytoskeleton 3.58 C-microtubule cytoskeleton 1.54 F-structural protein *F 19226 P-transmission of nerve impulse 35 1.66182 0.511688 0.0279254 FBgn0008635 42.91 P-vesicle transport 0.77 C-cellular_component 0.84 P-intracellular protein traffic *F 7268 P-synaptic transmission 35 1.66182 0.511688 0.0279254 FBgn0008635 42.91 P-vesicle transport 0.77 C-cellular_component 0.84 P-intracellular protein traffic *F 16043 P-cell organization and biogenesis 1 1.66105 0.636613 0.0606736 FBgn0038565 4.78 C-cytoskeleton 3.57 C-microtubule cytoskeleton 1.54 F-structural protein *F 7267 P-cell-cell signaling 35 1.65885 0.512392 0.0281838 FBgn0008635 42.54 P-vesicle transport 0.78 C-cellular_component 0.84 P-intracellular protein traffic *F 5681 C-spliceosome 15 1.65693 0.502254 0.0262422 FBgn0003659 4386.01 P-RNA splicing 0.01 P-mRNA splicing 0.52 F-RNA binding *F 67 P-DNA replication and chromosome cycle 8 1.65327 0.504046 0.0267301 FBgn0025458 21.24 P-phosphorylation 0.05 P-phosphate metabolism 14.89 P-nuclear division *F 7165 P-signal transduction 2 1.64859 0.649608 0.0659174 FBgn0004366 5729.99 P-phosphate metabolism 0 P-dephosphorylation 46.33 P-perception of abiotic stimulus *F 7345 P-embryogenesis and morphogenesis 6 1.64009 0.622473 0.057544 FBgn0000634 12.22 P-cell communication 1.97 C-plasma membrane/cell membrane 0.71 P-phosphate metabolism *F 5198 F-structural protein 7 1.63705 0.697735 0.084918 FBgn0039857 15.29 C-ribosome 0.89 P-phosphate metabolism 1.13 C-cell *F 15630 C-microtubule cytoskeleton 14 1.63689 0.521764 0.0310456 FBgn0002673 0 P-phosphate metabolism 208.25 P-microtubule-based process 129.87 P-dephosphorylation *F 7275 P-developmental processes 9 1.62545 0.72817 0.0990832 FBgn0004837 5.35 P-cell surface receptor linked signal transduction 4.08 P-cell communication 0.39 P-signal transduction *F 15630 C-microtubule cytoskeleton 15 1.62101 0.518638 0.0310456 FBgn0015625 61.91 P-microtubule-based process 0.39 P-M phase 1.82 P-nuclear division *F 7345 P-embryogenesis and morphogenesis 7 1.61609 0.617409 0.057544 FBgn0026193 22969.56 P-phosphate metabolism 0 P-phosphorylation 5.95 P-gametogenesis *F 6952 P-defense response 9 1.61545 0.539586 0.0360579 FBgn0039298 22.83 P-stress response 1.15 P-phosphate metabolism 1.12 C-intracellular/protoplasm *F 4930 F-G-protein coupled receptor/G-protein linked receptor 8 1.6117 0.590484 0.0496592 FBgn0011463 6.19 P-perception of chemical substance 6.19 P-chemosensory perception 4.09 P-perception of abiotic stimulus *F 4930 F-G-protein coupled receptor/G-protein linked receptor 9 1.6117 0.590484 0.0496592 FBgn0024917 6.19 P-perception of chemical substance 6.19 P-chemosensory perception 4.09 P-perception of abiotic stimulus *F 4930 F-G-protein coupled receptor/G-protein linked receptor 10 1.6117 0.590484 0.0496592 FBgn0044025 6.19 P-perception of chemical substance 6.19 P-chemosensory perception 4.09 P-perception of abiotic stimulus *F 4930 F-G-protein coupled receptor/G-protein linked receptor 11 1.6117 0.590484 0.0496592 FBgn0044026 6.19 P-perception of chemical substance 6.19 P-chemosensory perception 4.09 P-perception of abiotic stimulus *F 5524 F-ATP binding 4 1.61032 0.667518 0.0753356 FBgn0004402 4.6 F-hydrolase, acting on acid anhydrides 4.6 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.95 F-hydrolase *F 5515 F-protein binding 3 1.60905 0.672791 0.0774462 FBgn0011743 5.21 P-cytoskeleton organization and biogenesis 2.88 P-cell organization and biogenesis 2.35 C-cytoskeleton *F 16192 P-vesicle transport 3 1.59573 0.53561 0.0360579 FBgn0013762 6.04 P-neurotransmitter release 6.04 P-neurotransmitter maintenance 0.76 C-cellular_component *F 7345 P-embryogenesis and morphogenesis 8 1.59559 0.613058 0.057544 FBgn0004366 32057.99 P-phosphate metabolism 0 P-dephosphorylation 13.75 P-cell communication *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 10 1.59532 0.721091 0.0988553 FBgn0000570 10.83 C-nucleoplasm 2.37 C-intracellular/protoplasm 0.54 C-nucleus *F 5198 F-structural protein 8 1.59011 0.687536 0.084918 FBgn0036853 15.29 C-ribosome 0.89 P-phosphate metabolism 1.13 C-cell *F 5198 F-structural protein 9 1.59011 0.687536 0.084918 FBgn0029973 15.29 C-ribosome 0.89 P-phosphate metabolism 1.13 C-cell *F 6886 P-intracellular protein traffic 3 1.5763 0.539381 0.0379315 FBgn0013762 6.17 P-neurotransmitter release 6.17 P-neurotransmitter maintenance 0.77 C-cellular_component *F 5739 C-mitochondrion 2 1.57586 0.689214 0.086896 FBgn0015806 23.92 C-ribosome 0.61 F-phosphotransferase, alcohol group as acceptor 1.58 F-transferase *F 5856 C-cytoskeleton 10 1.57484 0.570344 0.0461318 FBgn0015625 13.86 P-microtubule-based process 0.34 P-M phase 2.33 P-cytoskeleton organization and biogenesis *F 5856 C-cytoskeleton 11 1.5733 0.570021 0.0461318 FBgn0000183 13.86 P-microtubule-based process 0.26 P-gametogenesis 2.52 P-developmental processes *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 11 1.57094 0.715721 0.0988553 FBgn0004237 10.83 C-nucleoplasm 1.62 F-nucleic acid binding 0.75 C-nucleus *F 7398 P-ectoderm development 4 1.56232 0.542567 0.0394457 FBgn0000634 15.96 P-cell communication 1.65 C-plasma membrane/cell membrane 0.77 P-phosphate metabolism *F 5856 C-cytoskeleton 12 1.56094 0.56743 0.0461318 FBgn0023177 0.02 P-phosphate metabolism 37.31 P-dephosphorylation 30.03 P-microtubule-based process *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 12 1.56072 0.713457 0.0988553 FBgn0030322 7.91 C-nucleoplasm 1.99 F-DNA binding 1.25 F-nucleic acid binding *F 5739 C-mitochondrion 3 1.55361 0.684299 0.086896 FBgn0017546 9.51 C-ribosome 1.88 F-structural protein of ribosome/ribosomal protein 0.8 C-membrane *F 5739 C-mitochondrion 4 1.55361 0.684299 0.086896 FBgn0003273 9.51 C-ribosome 1.88 F-structural protein of ribosome/ribosomal protein 0.8 C-membrane *F 9581 P-perception of external stimulus 4 1.5523 0.569053 0.0470977 FBgn0004244 43.13 P-response to abiotic stimulus 0.59 P-metabolism 0.91 F-ion channel *F 15031 P-protein transport 3 1.54898 0.545218 0.0408319 FBgn0013762 6.12 P-neurotransmitter release 6.12 P-neurotransmitter maintenance 1.3 C-cell *F 7275 P-developmental processes 10 1.54599 0.710681 0.0990832 FBgn0033881 36.35 P-cell communication 0.14 P-signal transduction 1.92 P-cell growth and/or maintenance *F 4674 F-protein serine/threonine kinase 45 1.54576 0.565759 0.0465586 FBgn0004839 37.91 P-protein amino acid phosphorylation 0.34 F-transmembrane receptor 1.84 P-phosphate metabolism *F 4930 F-G-protein coupled receptor/G-protein linked receptor 12 1.53931 0.575214 0.0496592 FBgn0016930 6.19 P-perception of chemical substance 6.19 P-chemosensory perception 4.09 P-perception of abiotic stimulus *F 5654 C-nucleoplasm 11 1.53302 0.541174 0.0406443 FBgn0005677 6.04 P-transcription 1.89 F-RNA polymerase II transcription factor 1.64 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 7397 P-histogenesis and organogenesis 5 1.53232 0.560638 0.0459198 FBgn0004366 2578.51 P-phosphate metabolism 0 P-dephosphorylation 17.66 P-cell communication *F 7165 P-signal transduction 3 1.52989 0.623998 0.0659174 FBgn0002936 79.04 P-perception of abiotic stimulus 0.11 P-perception of external stimulus 5.7 C-cell *F 5515 F-protein binding 4 1.51768 0.652707 0.0774462 FBgn0001624 3.38 P-transmission of nerve impulse 3.38 P-synaptic transmission 2.16 C-cytoskeleton *F 3735 F-structural protein of ribosome/ribosomal protein 10 1.51388 0.521999 0.0368129 FBgn0000002 23.94 C-ribosome 1.15 C-cytosolic ribosome 1.05 F-nucleic acid binding *F 16817 F-hydrolase, acting on acid anhydrides 6 1.51384 0.702964 0.0988553 FBgn0025615 8.58 F-ATP binding 2.15 F-purine nucleotide binding 0.95 F-GTP binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 6 1.51343 0.702871 0.0988553 FBgn0025615 8.58 F-ATP binding 2.15 F-purine nucleotide binding 0.95 F-GTP binding *F 4888 F-transmembrane receptor 7 1.50929 0.695436 0.0959401 FBgn0020277 99.12 P-perception of external stimulus 0.02 P-perception of abiotic stimulus 0.12 P-sensory perception *F 7398 P-ectoderm development 5 1.50767 0.531227 0.0394457 FBgn0004366 2790.31 P-phosphate metabolism 0 P-dephosphorylation 18.18 P-cell communication *F 5654 C-nucleoplasm 12 1.50409 0.535124 0.0406443 FBgn0004407 3.01 P-cell cycle/cell-division cycle 2.56 P-DNA replication and chromosome cycle 2.4 F-transferase, transferring phosphorus-containing groups *F 7275 P-developmental processes 11 1.50073 0.700502 0.0990832 FBgn0020503 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 4930 F-G-protein coupled receptor/G-protein linked receptor 13 1.49648 0.566041 0.0496592 FBgn0020278 39.92 P-G-protein coupled receptor protein signaling pathway 0.73 P-cell surface receptor linked signal transduction 1.37 C-intracellular/protoplasm *F 5654 C-nucleoplasm 13 1.49423 0.533052 0.0406443 FBgn0038903 5.17 P-transcription 1.85 F-transferase, transferring phosphorus-containing groups 1.54 P-nucleobase\, nucleoside\, nucleotide and nucleic acid metabolism *F 16021 C-integral membrane protein 2 1.49363 0.686935 0.0937562 FBgn0027498 3.67 F-G-protein coupled receptor/G-protein linked receptor 2.14 P-G-protein coupled receptor protein signaling pathway 1.55 F-transmembrane receptor *F 3735 F-structural protein of ribosome/ribosomal protein 11 1.49265 0.517597 0.0368129 FBgn0013686 23.94 C-ribosome 1.16 C-mitochondrion 0.95 C-cytoplasm *F 3735 F-structural protein of ribosome/ribosomal protein 12 1.49265 0.517597 0.0368129 FBgn0013688 23.94 C-ribosome 1.16 C-mitochondrion 0.95 C-cytoplasm *F 4674 F-protein serine/threonine kinase 46 1.48986 0.553872 0.0465586 FBgn0015380 37.91 P-protein amino acid phosphorylation 0.54 F-transmembrane receptor 1.84 P-phosphate metabolism *F 7397 P-histogenesis and organogenesis 6 1.4829 0.550118 0.0459198 FBgn0020440 71.73 P-phosphate metabolism 0.01 P-phosphorylation 5.83 P-cell communication *F 6796 P-phosphate metabolism 1 1.47805 0.660646 0.0841395 FBgn0020392 18.11 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 4888 F-transmembrane receptor 8 1.47632 0.687945 0.0959401 FBgn0015608 14.58 F-ion channel 3.93 C-integral membrane protein 3.14 C-plasma membrane/cell membrane *F 4888 F-transmembrane receptor 9 1.46716 0.68585 0.0959401 FBgn0004435 345.1 P-perception of external stimulus 0.01 P-perception of abiotic stimulus 16.65 P-G-protein coupled receptor protein signaling pathway *F 7010 P-cytoskeleton organization and biogenesis 2 1.4654 0.552786 0.0477529 FBgn0001313 3.64 C-cytoskeleton 3.27 C-microtubule cytoskeleton 2.01 C-microtubule associated protein *F 7010 P-cytoskeleton organization and biogenesis 3 1.4654 0.552786 0.0477529 FBgn0039246 3.64 C-cytoskeleton 3.27 C-microtubule cytoskeleton 2.01 C-microtubule associated protein *F 5654 C-nucleoplasm 14 1.4642 0.526706 0.0406443 FBgn0040305 5.17 P-transcription 2.02 F-RNA polymerase II transcription factor 1.54 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 7010 P-cytoskeleton organization and biogenesis 4 1.45809 0.551204 0.0477529 FBgn0003887 3.8 C-cytoskeleton 3.4 C-microtubule cytoskeleton 1.95 P-developmental processes *F 5739 C-mitochondrion 5 1.45439 0.66193 0.086896 FBgn0011285 18.95 C-ribosome 0.72 P-protein modification 0.76 F-phosphotransferase, alcohol group as acceptor *F 9581 P-perception of external stimulus 5 1.44948 0.547061 0.0470977 FBgn0000473 35.96 P-response to abiotic stimulus 0.62 P-metabolism 0.92 P-protein metabolism and modification *F 5524 F-ATP binding 5 1.44934 0.631816 0.0753356 FBgn0037900 4.25 F-hydrolase, acting on acid anhydrides 4.25 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.96 F-hydrolase *F 5524 F-ATP binding 6 1.44934 0.631816 0.0753356 FBgn0031700 4.25 F-hydrolase, acting on acid anhydrides 4.25 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.96 F-hydrolase *F 6810 P-transport 3 1.44902 0.584495 0.0586138 FBgn0013762 0.11 P-phosphate metabolism 5.9 P-phosphorylation 5.58 P-neurotransmitter release *F 4888 F-transmembrane receptor 10 1.43296 0.677963 0.0959401 FBgn0004603 48.37 P-perception of external stimulus 0.03 P-perception of abiotic stimulus 6.44 F-protein kinase *F 7275 P-developmental processes 12 1.43008 0.684281 0.0990832 FBgn0003520 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.57 P-cell growth and/or maintenance *F 3702 F-RNA polymerase II transcription factor 3 1.42614 0.609123 0.0688652 FBgn0025455 5.44 C-nucleus 0.41 F-transferase, transferring phosphorus-containing groups 1.99 F-kinase *F 5386 F-carrier/carrier type transporter 2 1.42113 0.677698 0.097051 FBgn0038309 3.91 F-cation transporter 3.59 F-ion transporter 0.9 P-phosphate metabolism *F 5386 F-carrier/carrier type transporter 3 1.42113 0.677698 0.097051 FBgn0038407 3.91 F-cation transporter 3.59 F-ion transporter 0.9 P-phosphate metabolism *F 5386 F-carrier/carrier type transporter 4 1.42113 0.677698 0.097051 FBgn0039176 3.91 F-cation transporter 3.59 F-ion transporter 0.9 P-phosphate metabolism *F 5386 F-carrier/carrier type transporter 5 1.42113 0.677698 0.097051 FBgn0034956 3.91 F-cation transporter 3.59 F-ion transporter 0.9 P-phosphate metabolism *F 5386 F-carrier/carrier type transporter 6 1.42113 0.677698 0.097051 FBgn0038719 3.91 F-cation transporter 3.59 F-ion transporter 0.9 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 12 1.41906 0.641946 0.0820352 FBgn0000116 16.02 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 16773 F-phosphotransferase, alcohol group as acceptor 13 1.41906 0.641946 0.0820352 FBgn0032224 16.02 F-kinase 0.98 P-phosphorylation 1.02 P-phosphate metabolism *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 13 1.41728 0.680794 0.0988553 FBgn0036188 9.83 C-nucleoplasm 1.64 F-transferase\, transferring phosphorus-containing groups 0.77 C-nucleus *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 14 1.41728 0.680794 0.0988553 FBgn0035644 9.83 C-nucleoplasm 1.64 F-transferase\, transferring phosphorus-containing groups 0.77 C-nucleus *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 15 1.41728 0.680794 0.0988553 FBgn0027903 9.83 C-nucleoplasm 1.64 F-transferase\, transferring phosphorus-containing groups 0.77 C-nucleus *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 16 1.41728 0.680794 0.0988553 FBgn0034536 9.83 C-nucleoplasm 1.64 F-transferase\, transferring phosphorus-containing groups 0.77 C-nucleus *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 17 1.41728 0.680794 0.0988553 FBgn0038951 9.83 C-nucleoplasm 1.64 F-transferase\, transferring phosphorus-containing groups 0.77 C-nucleus *F 4674 F-protein serine/threonine kinase 47 1.4075 0.536033 0.0465586 FBgn0003366 37.91 P-protein amino acid phosphorylation 0.47 F-transmembrane receptor 1.84 P-phosphate metabolism *F 7275 P-developmental processes 13 1.40654 0.678783 0.0990832 FBgn0003169 87.94 P-phosphate metabolism 0.01 P-phosphorylation 9.27 P-cell surface receptor linked signal transduction *F 4888 F-transmembrane receptor 11 1.37933 0.6654 0.0959401 FBgn0039840 7.97 F-ion channel 2.68 C-integral membrane protein 1.82 C-plasma membrane/cell membrane *F 4888 F-transmembrane receptor 12 1.37933 0.6654 0.0959401 FBgn0029733 7.97 F-ion channel 2.68 C-integral membrane protein 1.82 C-plasma membrane/cell membrane *F 4888 F-transmembrane receptor 13 1.37933 0.6654 0.0959401 FBgn0033558 7.97 F-ion channel 2.68 C-integral membrane protein 1.82 C-plasma membrane/cell membrane *F 4888 F-transmembrane receptor 14 1.37933 0.6654 0.0959401 FBgn0036542 7.97 F-ion channel 2.68 C-integral membrane protein 1.82 C-plasma membrane/cell membrane *F 4888 F-transmembrane receptor 15 1.37933 0.6654 0.0959401 FBgn0038726 7.97 F-ion channel 2.68 C-integral membrane protein 1.82 C-plasma membrane/cell membrane *F 5198 F-structural protein 10 1.37446 0.638606 0.084918 FBgn0013686 11.85 C-ribosome 1.2 C-cell 0.88 C-cellular_component *F 5198 F-structural protein 11 1.37446 0.638606 0.084918 FBgn0013688 11.85 C-ribosome 1.2 C-cell 0.88 C-cellular_component *F 4930 F-G-protein coupled receptor/G-protein linked receptor 14 1.37266 0.538901 0.0496592 FBgn0011293 5.79 P-perception of chemical substance 5.79 P-chemosensory perception 3.85 P-perception of abiotic stimulus *F 15290 F-electrochemical potential-driven transporter 6 1.35953 0.506657 0.0413048 FBgn0029146 4.76 F-cation transporter 3.42 F-ion transporter 0.9 P-phosphate metabolism *F 15291 F-porter 6 1.35953 0.506657 0.0413048 FBgn0029146 4.76 F-cation transporter 3.42 F-ion transporter 0.9 P-phosphate metabolism *F 5856 C-cytoskeleton 13 1.34859 0.521541 0.0461318 FBgn0002673 0 P-phosphate metabolism 134.17 P-dephosphorylation 30.03 P-microtubule-based process *F 5654 C-nucleoplasm 15 1.34381 0.500763 0.0406443 FBgn0030556 31.14 P-mRNA processing 0.23 P-RNA metabolism 0.23 P-dephosphorylation *F 5198 F-structural protein 12 1.34152 0.630813 0.084918 FBgn0000002 11.85 C-ribosome 0.89 P-phosphate metabolism 0.91 C-cytosol *F 6996 P-organelle organization and biogenesis 2 1.33777 0.555705 0.0574116 FBgn0003887 3.86 C-cytoskeleton 2.9 C-microtubule cytoskeleton 1.88 P-developmental processes *F 6996 P-organelle organization and biogenesis 3 1.33307 0.554626 0.0574116 FBgn0001313 3.7 C-cytoskeleton 2.81 C-microtubule cytoskeleton 1.89 C-microtubule associated protein *F 6996 P-organelle organization and biogenesis 4 1.33307 0.554626 0.0574116 FBgn0039246 3.7 C-cytoskeleton 2.81 C-microtubule cytoskeleton 1.89 C-microtubule associated protein *F 4930 F-G-protein coupled receptor/G-protein linked receptor 15 1.33054 0.529447 0.0496592 FBgn0024941 32.83 P-G-protein coupled receptor protein signaling pathway 1.37 C-intracellular/protoplasm 0.74 P-cell surface receptor linked signal transduction *F 7028 P-cytoplasm organization and biogenesis 2 1.32456 0.560376 0.0599791 FBgn0003887 3.75 C-cytoskeleton 2.97 C-microtubule cytoskeleton 1.85 P-developmental processes *F 7028 P-cytoplasm organization and biogenesis 3 1.32239 0.559872 0.0599791 FBgn0001313 3.59 C-cytoskeleton 2.87 C-microtubule cytoskeleton 1.87 C-microtubule associated protein *F 7028 P-cytoplasm organization and biogenesis 4 1.32239 0.559872 0.0599791 FBgn0039246 3.59 C-cytoskeleton 2.87 C-microtubule cytoskeleton 1.87 C-microtubule associated protein *F 7345 P-embryogenesis and morphogenesis 9 1.3142 0.55068 0.057544 FBgn0000313 5.62 P-cell communication 2.3 C-plasma membrane/cell membrane 0.71 P-phosphate metabolism *F 16043 P-cell organization and biogenesis 2 1.31095 0.559266 0.0606736 FBgn0003887 3.75 C-cytoskeleton 2.96 C-microtubule cytoskeleton 1.84 P-developmental processes *F 16043 P-cell organization and biogenesis 3 1.30968 0.558971 0.0606736 FBgn0001313 3.59 C-cytoskeleton 2.87 C-microtubule cytoskeleton 1.86 C-microtubule associated protein *F 16043 P-cell organization and biogenesis 4 1.30968 0.558971 0.0606736 FBgn0039246 3.59 C-cytoskeleton 2.87 C-microtubule cytoskeleton 1.86 C-microtubule associated protein *F 3723 F-RNA binding 3 1.30882 0.567055 0.0635331 FBgn0000426 22507.67 P-RNA splicing 0 P-mRNA splicing 0.05 C-spliceosome *F 4888 F-transmembrane receptor 16 1.29405 0.644912 0.0959401 FBgn0016930 48.37 P-perception of external stimulus 0.03 P-perception of abiotic stimulus 6.44 F-protein kinase *F 7345 P-embryogenesis and morphogenesis 10 1.28652 0.544257 0.057544 FBgn0028436 18 P-cell communication 0.17 P-signal transduction 5.19 P-cell surface receptor linked signal transduction *F 7397 P-histogenesis and organogenesis 7 1.28395 0.506295 0.0459198 FBgn0004657 31.93 P-cell communication 0.25 C-integral plasma membrane protein 2.38 C-plasma membrane/cell membrane *F 7049 P-cell cycle/cell-division cycle 1 1.28256 0.504169 0.0453942 FBgn0027620 4.35 P-DNA metabolism 2.17 C-nucleoplasm 1.49 F-molecular_function *F 7275 P-developmental processes 14 1.27446 0.647039 0.0990832 FBgn0000499 5.81 P-cell surface receptor linked signal transduction 4.56 P-cell communication 0.33 P-cell organization and biogenesis *F 7345 P-embryogenesis and morphogenesis 11 1.27126 0.540691 0.057544 FBgn0004837 5.62 P-cell communication 4.8 P-cell surface receptor linked signal transduction 0.25 P-signal transduction *F 16021 C-integral membrane protein 3 1.26964 0.634033 0.0937562 FBgn0004620 2.23 F-transmembrane receptor 2.1 F-cation channel 1.62 C-plasma membrane/cell membrane *F 5886 C-plasma membrane/cell membrane 2 1.26566 0.642881 0.0981748 FBgn0035635 0.2 P-response to biotic stimulus 3.19 C-integral membrane protein 3.14 F-transmembrane receptor *F 4930 F-G-protein coupled receptor/G-protein linked receptor 16 1.26004 0.513358 0.0496592 FBgn0029904 5.79 P-perception of chemical substance 5.79 P-chemosensory perception 3.85 P-perception of abiotic stimulus *F 3702 F-RNA polymerase II transcription factor 4 1.256 0.569353 0.0688652 FBgn0003276 9.27 C-nucleus 0.17 F-transferase, transferring phosphorus-containing groups 2.53 P-transcription *F 3702 F-RNA polymerase II transcription factor 5 1.256 0.569353 0.0688652 FBgn0003277 9.27 C-nucleus 0.17 F-transferase, transferring phosphorus-containing groups 2.53 P-transcription *F 9605 P-response to external stimulus 5 1.25519 0.630469 0.0937562 FBgn0028427 5.03 P-cell surface receptor linked signal transduction 0.35 P-phosphorylation 2.6 P-signal transduction *F 5886 C-plasma membrane/cell membrane 3 1.2539 0.639967 0.0981748 FBgn0004624 66.02 P-cell-cell signaling 6.22 P-phosphorylation 0.17 P-phosphate metabolism *F 7275 P-developmental processes 15 1.2516 0.641382 0.0990832 FBgn0039234 4.24 P-cell surface receptor linked signal transduction 3 P-cell communication 0.45 P-signal transduction *F 3702 F-RNA polymerase II transcription factor 6 1.25078 0.568097 0.0688652 FBgn0015396 6.59 C-nucleus 2.35 P-histogenesis and organogenesis 0.53 P-cell cycle/cell-division cycle *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 18 1.2485 0.640114 0.0988553 FBgn0017462 10.83 C-nucleoplasm 0.43 F-transcription factor 2.08 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 19 1.2485 0.640114 0.0988553 FBgn0017463 10.83 C-nucleoplasm 0.43 F-transcription factor 2.08 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 20 1.2485 0.640114 0.0988553 FBgn0017464 10.83 C-nucleoplasm 0.43 F-transcription factor 2.08 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 21 1.2485 0.640114 0.0988553 FBgn0017465 10.83 C-nucleoplasm 0.43 F-transcription factor 2.08 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 22 1.2485 0.640114 0.0988553 FBgn0017466 10.83 C-nucleoplasm 0.43 F-transcription factor 2.08 F-DNA binding *F 7345 P-embryogenesis and morphogenesis 12 1.24263 0.533953 0.057544 FBgn0011274 23.4 P-cell communication 0.25 P-defense response 3.86 P-cell surface receptor linked signal transduction *F 7010 P-cytoskeleton organization and biogenesis 5 1.23802 0.501986 0.0477529 FBgn0034155 2.72 C-cytoskeleton 2.56 C-microtubule cytoskeleton 1.75 C-microtubule associated protein *F 7275 P-developmental processes 16 1.22711 0.635266 0.0990832 FBgn0000634 7.69 P-cell communication 1.77 C-plasma membrane/cell membrane 0.72 P-cell growth and/or maintenance *F 7275 P-developmental processes 17 1.22524 0.634797 0.0990832 FBgn0010798 5.35 P-cell surface receptor linked signal transduction 4.08 P-cell communication 0.39 P-signal transduction *F 7275 P-developmental processes 18 1.22524 0.634797 0.0990832 FBgn0043964 5.35 P-cell surface receptor linked signal transduction 4.08 P-cell communication 0.39 P-signal transduction *F 16043 P-cell organization and biogenesis 5 1.22516 0.539019 0.0606736 FBgn0034155 2.69 C-cytoskeleton 2.3 C-microtubule cytoskeleton 1.67 F-protein binding *F 7028 P-cytoplasm organization and biogenesis 5 1.21979 0.535713 0.0599791 FBgn0034155 2.69 C-cytoskeleton 2.31 C-microtubule cytoskeleton 1.65 C-microtubule associated protein *F 6996 P-organelle organization and biogenesis 5 1.21711 0.5275 0.0574116 FBgn0034155 2.75 C-cytoskeleton 2.26 C-microtubule cytoskeleton 1.66 C-microtubule associated protein *F 7275 P-developmental processes 19 1.21041 0.631061 0.0990832 FBgn0004366 3750.15 P-phosphate metabolism 0 P-dephosphorylation 8.46 P-cell communication *F 5515 F-protein binding 5 1.20195 0.578508 0.0774462 FBgn0013813 4.55 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.37 C-microtubule cytoskeleton *F 5386 F-carrier/carrier type transporter 7 1.20108 0.624262 0.097051 FBgn0004363 13.68 C-mitochondrial membrane 0.81 F-alpha-type channel 0.81 F-channel/pore class transporter *F 4888 F-transmembrane receptor 17 1.20033 0.621624 0.0959401 FBgn0004864 6.44 F-protein kinase 3.02 P-cell communication 2.51 P-phosphate metabolism *F 5386 F-carrier/carrier type transporter 8 1.1934 0.622315 0.097051 FBgn0029146 3.46 F-cation transporter 3.18 F-ion transporter 0.9 P-phosphate metabolism *F 7345 P-embryogenesis and morphogenesis 13 1.19272 0.522056 0.057544 FBgn0000258 32057.99 P-phosphate metabolism 0 P-phosphorylation 5.62 P-cell communication *F 6796 P-phosphate metabolism 2 1.19186 0.592374 0.0841395 FBgn0004908 25.39 P-protein modification 0.52 F-purine nucleotide binding 1.76 F-guanyl nucleotide binding *F 5886 C-plasma membrane/cell membrane 4 1.18537 0.622732 0.0981748 FBgn0015774 5.26 P-cell communication 2.2 P-embryogenesis and morphogenesis 1.44 C-extracellular *F 3702 F-RNA polymerase II transcription factor 7 1.18289 0.551554 0.0688652 FBgn0010422 5.68 C-nucleus 1.66 P-transcription 1.36 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism *F 5739 C-mitochondrion 6 1.18253 0.596542 0.086896 FBgn0026207 22.35 C-membrane 0.62 C-nucleus 0.64 P-transport *F 7275 P-developmental processes 20 1.18199 0.623843 0.0990832 FBgn0024180 23.55 P-microtubule-based process 0.18 P-cell organization and biogenesis 3.71 P-cell growth and/or maintenance *F 7275 P-developmental processes 21 1.18091 0.623567 0.0990832 FBgn0037375 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 22 1.16914 0.62055 0.0990832 FBgn0024846 87.94 P-phosphate metabolism 0.01 P-phosphorylation 67.11 P-cell communication *F 7275 P-developmental processes 23 1.16063 0.618362 0.0990832 FBgn0000140 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 24 1.1564 0.617272 0.0990832 FBgn0039533 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 23 1.15194 0.615627 0.0988553 FBgn0038499 9.83 C-nucleoplasm 0.37 F-transcription factor 2.44 F-DNA binding *F 7275 P-developmental processes 25 1.13776 0.61244 0.0990832 FBgn0000313 4.08 P-cell communication 2.02 C-plasma membrane/cell membrane 1.45 F-molecular_function *F 7345 P-embryogenesis and morphogenesis 14 1.13499 0.508046 0.057544 FBgn0004657 23.61 P-cell communication 0.23 C-integral plasma membrane protein 2.81 C-plasma membrane/cell membrane *F 7275 P-developmental processes 26 1.13458 0.611613 0.0990832 FBgn0002645 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 27 1.13362 0.611364 0.0990832 FBgn0010342 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 28 1.13137 0.610777 0.0990832 FBgn0024326 16132.81 P-phosphate metabolism 0 P-phosphorylation 0.08 P-metabolism *F 9605 P-response to external stimulus 6 1.12874 0.598422 0.0937562 FBgn0000119 3.75 P-cell surface receptor linked signal transduction 2.17 P-signal transduction 1.72 F-receptor *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 24 1.12415 0.608402 0.0988553 FBgn0022356 10.83 C-nucleoplasm 0.43 F-transcription factor 2.08 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 25 1.12083 0.607532 0.0988553 FBgn0000212 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 7345 P-embryogenesis and morphogenesis 15 1.12061 0.504512 0.057544 FBgn0002968 8.36 P-cell communication 1.76 C-plasma membrane/cell membrane 0.71 P-phosphate metabolism *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 26 1.11879 0.606998 0.0988553 FBgn0028897 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 27 1.11879 0.606998 0.0988553 FBgn0025463 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 28 1.11879 0.606998 0.0988553 FBgn0033562 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 7 1.11508 0.606023 0.0988553 FBgn0029682 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 8 1.11508 0.606023 0.0988553 FBgn0032031 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 9 1.11508 0.606023 0.0988553 FBgn0032818 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 10 1.11508 0.606023 0.0988553 FBgn0032882 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 11 1.11508 0.606023 0.0988553 FBgn0028473 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 12 1.11508 0.606023 0.0988553 FBgn0039339 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 13 1.11508 0.606023 0.0988553 FBgn0029959 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 14 1.11508 0.606023 0.0988553 FBgn0030792 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 15 1.11508 0.606023 0.0988553 FBgn0027836 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 16 1.11508 0.606023 0.0988553 FBgn0038723 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 17 1.11508 0.606023 0.0988553 FBgn0037391 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 18 1.11508 0.606023 0.0988553 FBgn0030151 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 19 1.11508 0.606023 0.0988553 FBgn0038106 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 20 1.11508 0.606023 0.0988553 FBgn0034610 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 21 1.11508 0.606023 0.0988553 FBgn0033914 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 22 1.11508 0.606023 0.0988553 FBgn0015792 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 23 1.11508 0.606023 0.0988553 FBgn0025985 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 7 1.11505 0.606016 0.0988553 FBgn0029682 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 8 1.11505 0.606016 0.0988553 FBgn0032031 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 9 1.11505 0.606016 0.0988553 FBgn0032818 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 10 1.11505 0.606016 0.0988553 FBgn0032882 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 11 1.11505 0.606016 0.0988553 FBgn0028473 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 12 1.11505 0.606016 0.0988553 FBgn0039339 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 13 1.11505 0.606016 0.0988553 FBgn0029959 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 14 1.11505 0.606016 0.0988553 FBgn0030792 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 15 1.11505 0.606016 0.0988553 FBgn0027836 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 16 1.11505 0.606016 0.0988553 FBgn0038723 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 17 1.11505 0.606016 0.0988553 FBgn0037391 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 18 1.11505 0.606016 0.0988553 FBgn0030151 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 19 1.11505 0.606016 0.0988553 FBgn0038106 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 20 1.11505 0.606016 0.0988553 FBgn0034610 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 21 1.11505 0.606016 0.0988553 FBgn0033914 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 22 1.11505 0.606016 0.0988553 FBgn0015792 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 23 1.11505 0.606016 0.0988553 FBgn0025985 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 7275 P-developmental processes 29 1.11132 0.605526 0.0990832 FBgn0021874 4.98 P-cell surface receptor linked signal transduction 3.71 P-cell communication 0.4 P-signal transduction *F 5886 C-plasma membrane/cell membrane 5 1.1103 0.6033 0.0981748 FBgn0027343 3.19 C-integral membrane protein 2.51 P-cell communication 2.31 F-transmembrane receptor *F 7275 P-developmental processes 30 1.10954 0.60506 0.0990832 FBgn0001612 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 31 1.10954 0.60506 0.0990832 FBgn0000283 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 4888 F-transmembrane receptor 18 1.10662 0.597464 0.0959401 FBgn0029904 41.04 P-perception of external stimulus 0.04 P-perception of abiotic stimulus 13.9 F-ion channel *F 7275 P-developmental processes 32 1.10031 0.602624 0.0990832 FBgn0027948 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 5886 C-plasma membrane/cell membrane 6 1.09924 0.600384 0.0981748 FBgn0010399 0.06 F-ion channel 3.84 F-transmembrane receptor 3.52 F-channel/pore class transporter *F 9605 P-response to external stimulus 7 1.09843 0.590495 0.0937562 FBgn0004514 0.03 P-G-protein coupled receptor protein signaling pathway 31.73 F-G-protein coupled receptor/G-protein linked receptor 2.68 P-cell surface receptor linked signal transduction *F 16773 F-phosphotransferase, alcohol group as acceptor 14 1.09782 0.563333 0.0820352 FBgn0020615 12.82 F-kinase 0.96 P-metabolism 0.97 P-biosynthesis/anabolism *F 7275 P-developmental processes 33 1.0971 0.601777 0.0990832 FBgn0026430 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 5524 F-ATP binding 7 1.09399 0.545924 0.0753356 FBgn0013349 4.6 F-hydrolase, acting on acid anhydrides 4.6 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.61 F-phosphatase *F 5515 F-protein binding 6 1.09395 0.55117 0.0774462 FBgn0024432 4.19 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.39 C-microtubule cytoskeleton *F 3723 F-RNA binding 4 1.09326 0.514712 0.0635331 FBgn0039566 6776.16 P-RNA splicing 0 P-mRNA splicing 0.07 C-spliceosome *F 5739 C-mitochondrion 7 1.0861 0.571714 0.086896 FBgn0039857 12 C-ribosome 0.8 C-membrane 0.9 F-carrier/carrier type transporter *F 4888 F-transmembrane receptor 19 1.08509 0.591782 0.0959401 FBgn0011463 48.37 P-perception of external stimulus 0.03 P-perception of abiotic stimulus 0.16 P-sensory perception *F 4888 F-transmembrane receptor 20 1.08509 0.591782 0.0959401 FBgn0024917 48.37 P-perception of external stimulus 0.03 P-perception of abiotic stimulus 0.16 P-sensory perception *F 4888 F-transmembrane receptor 21 1.08509 0.591782 0.0959401 FBgn0044025 48.37 P-perception of external stimulus 0.03 P-perception of abiotic stimulus 0.16 P-sensory perception *F 4888 F-transmembrane receptor 22 1.08509 0.591782 0.0959401 FBgn0044026 48.37 P-perception of external stimulus 0.03 P-perception of abiotic stimulus 0.16 P-sensory perception *F 7275 P-developmental processes 34 1.08198 0.597765 0.0990832 FBgn0011300 503.02 P-phosphate metabolism 0 P-phosphorylation 7.69 P-cell communication *F 7275 P-developmental processes 35 1.07163 0.595002 0.0990832 FBgn0040229 4.24 P-cell surface receptor linked signal transduction 3 P-cell communication 0.45 P-signal transduction *F 7275 P-developmental processes 36 1.07136 0.594932 0.0990832 FBgn0016797 7.69 P-cell communication 5.35 P-cell surface receptor linked signal transduction 0.29 P-signal transduction *F 5515 F-protein binding 7 1.0711 0.545242 0.0774462 FBgn0000046 3.59 P-cytoskeleton organization and biogenesis 2.35 C-cytoskeleton 2.24 P-cell organization and biogenesis *F 5515 F-protein binding 8 1.0711 0.545242 0.0774462 FBgn0038576 3.59 P-cytoskeleton organization and biogenesis 2.35 C-cytoskeleton 2.24 P-cell organization and biogenesis *F 5515 F-protein binding 9 1.0711 0.545242 0.0774462 FBgn0034216 3.59 P-cytoskeleton organization and biogenesis 2.35 C-cytoskeleton 2.24 P-cell organization and biogenesis *F 7165 P-signal transduction 4 1.06951 0.51518 0.0659174 FBgn0020430 4.45 F-G-protein coupled receptor/G-protein linked receptor 1.57 F-signal transducer 0.69 F-receptor *F 4888 F-transmembrane receptor 23 1.0677 0.587155 0.0959401 FBgn0003036 11.52 F-ion channel 3.39 C-integral membrane protein 0.39 F-cation channel *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 24 1.06536 0.592836 0.0988553 FBgn0017549 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 24 1.06502 0.592744 0.0988553 FBgn0017549 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 5886 C-plasma membrane/cell membrane 7 1.0641 0.591037 0.0981748 FBgn0027342 3.37 P-cell communication 3.19 C-integral membrane protein 2.31 F-transmembrane receptor *F 16021 C-integral membrane protein 4 1.06365 0.581279 0.0937562 FBgn0025936 3.25 F-transmembrane receptor 2.14 C-intracellular/protoplasm 1.99 C-plasma membrane/cell membrane *F 5515 F-protein binding 10 1.06159 0.542757 0.0774462 FBgn0040080 3.59 P-cytoskeleton organization and biogenesis 2.24 P-cell organization and biogenesis 2.19 P-M phase *F 16021 C-integral membrane protein 5 1.05605 0.579246 0.0937562 FBgn0004625 0.04 P-perception of abiotic stimulus 14.02 P-perception of external stimulus 3.07 P-cell communication *F 7275 P-developmental processes 37 1.05427 0.590343 0.0990832 FBgn0028902 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 38 1.05427 0.590343 0.0990832 FBgn0013718 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 39 1.05427 0.590343 0.0990832 FBgn0035638 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 40 1.05427 0.590343 0.0990832 FBgn0034957 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 41 1.05427 0.590343 0.0990832 FBgn0034282 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 42 1.05427 0.590343 0.0990832 FBgn0035716 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 43 1.05427 0.590343 0.0990832 FBgn0027066 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 44 1.05427 0.590343 0.0990832 FBgn0026568 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 7275 P-developmental processes 45 1.05427 0.590343 0.0990832 FBgn0026598 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 5886 C-plasma membrane/cell membrane 8 1.04601 0.586168 0.0981748 FBgn0003733 6.22 P-phosphorylation 0.17 P-phosphate metabolism 0.39 P-response to abiotic stimulus *F 16817 F-hydrolase, acting on acid anhydrides 25 1.03786 0.585419 0.0988553 FBgn0023444 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 25 1.03781 0.585406 0.0988553 FBgn0023444 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 5386 F-carrier/carrier type transporter 9 1.03368 0.580408 0.097051 FBgn0028868 6.76 C-mitochondrial membrane 1.78 F-ATPase 1.76 F-purine nucleotide binding *F 7275 P-developmental processes 46 1.03347 0.584714 0.0990832 FBgn0000119 8.62 P-cell surface receptor linked signal transduction 7.69 P-cell communication 0.29 P-signal transduction *F 7275 P-developmental processes 47 1.02513 0.582442 0.0990832 FBgn0013983 67.11 P-cell communication 0.03 P-stress response 18.95 P-cell growth and/or maintenance *F 6464 P-protein modification 1 1.02424 0.535534 0.0785236 FBgn0038308 2537.57 P-phosphate metabolism 0 P-phosphorylation 3.53 F-protein kinase *F 16817 F-hydrolase, acting on acid anhydrides 26 1.01545 0.579309 0.0988553 FBgn0021760 14.13 F-GTP binding 0.39 F-guanyl nucleotide binding 2.52 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 26 1.01534 0.579279 0.0988553 FBgn0021760 14.13 F-GTP binding 0.39 F-guanyl nucleotide binding 2.52 F-purine nucleotide binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 29 1.0149 0.579161 0.0988553 FBgn0040020 9.83 C-nucleoplasm 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 30 1.0149 0.579161 0.0988553 FBgn0030891 9.83 C-nucleoplasm 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 31 1.0149 0.579161 0.0988553 FBgn0037382 9.83 C-nucleoplasm 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 6796 P-phosphate metabolism 3 1.01382 0.546091 0.0841395 FBgn0038272 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 32 1.00265 0.575795 0.0988553 FBgn0039066 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 33 1.00265 0.575795 0.0988553 FBgn0023211 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 34 1.00265 0.575795 0.0988553 FBgn0023212 9.83 C-nucleoplasm 0.45 F-transcription factor 1.99 F-DNA binding *F 3702 F-RNA polymerase II transcription factor 8 1.00104 0.505177 0.0688652 FBgn0003275 9.27 C-nucleus 0.14 F-transferase, transferring phosphorus-containing groups 2.53 P-transcription *F 5386 F-carrier/carrier type transporter 10 0.993424 0.569391 0.097051 FBgn0010653 6.76 C-mitochondrial membrane 1.54 F-ATPase 1.51 F-purine nucleotide binding *F 5515 F-protein binding 11 0.992836 0.524514 0.0774462 FBgn0032520 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 12 0.992836 0.524514 0.0774462 FBgn0036195 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 13 0.992836 0.524514 0.0774462 FBgn0032657 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 14 0.992836 0.524514 0.0774462 FBgn0037726 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 15 0.992836 0.524514 0.0774462 FBgn0013809 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 16 0.992836 0.524514 0.0774462 FBgn0013810 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 17 0.992836 0.524514 0.0774462 FBgn0013811 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 18 0.992836 0.524514 0.0774462 FBgn0013812 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 19 0.992836 0.524514 0.0774462 FBgn0029993 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 20 0.992836 0.524514 0.0774462 FBgn0035100 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 21 0.992836 0.524514 0.0774462 FBgn0039510 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 22 0.992836 0.524514 0.0774462 FBgn0035256 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 23 0.992836 0.524514 0.0774462 FBgn0034566 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 5515 F-protein binding 24 0.992836 0.524514 0.0774462 FBgn0030276 3.86 C-cytoskeleton 3.59 P-cytoskeleton organization and biogenesis 0.41 C-microtubule cytoskeleton *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 35 0.98984 0.572253 0.0988553 FBgn0020388 9.83 C-nucleoplasm 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 16021 C-integral membrane protein 6 0.987164 0.560522 0.0937562 FBgn0011268 3.23 F-G-protein coupled receptor/G-protein linked receptor 1.47 F-transmembrane receptor 1.42 C-plasma membrane/cell membrane *F 3702 F-RNA polymerase II transcription factor 9 0.986082 0.501216 0.0688652 FBgn0032816 2.51 C-nucleus 1.74 P-transcription 1.5 C-nucleoplasm *F 6796 P-phosphate metabolism 4 0.984397 0.538111 0.0841395 FBgn0036106 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 6796 P-phosphate metabolism 5 0.984397 0.538111 0.0841395 FBgn0029818 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 5739 C-mitochondrion 8 0.983298 0.544147 0.086896 FBgn0040777 7.99 C-membrane 1.17 F-transporter 0.9 F-carrier/carrier type transporter *F 6796 P-phosphate metabolism 6 0.982729 0.537656 0.0841395 FBgn0041707 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 7275 P-developmental processes 48 0.980738 0.570209 0.0990832 FBgn0025936 503.02 P-phosphate metabolism 0 P-phosphorylation 9.11 F-protein kinase *F 5515 F-protein binding 25 0.975283 0.519773 0.0774462 FBgn0025441 3.59 P-cytoskeleton organization and biogenesis 2.35 C-cytoskeleton 2.24 P-cell organization and biogenesis *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 36 0.966289 0.565691 0.0988553 FBgn0023542 7.88 P-cytoplasm organization and biogenesis 1.41 P-transport 1.25 F-nucleic acid binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 37 0.96561 0.565501 0.0988553 FBgn0013755 9.83 C-nucleoplasm 1.67 C-intracellular/protoplasm 0.65 C-nucleus *F 5524 F-ATP binding 8 0.965278 0.511916 0.0753356 FBgn0027890 4.25 F-hydrolase, acting on acid anhydrides 4.25 F-hydrolase\, acting on acid anhydrides\, in phosphorus-containing anhydrides 0.62 F-phosphatase *F 9605 P-response to external stimulus 8 0.962591 0.553704 0.0937562 FBgn0016797 2.8 P-cell surface receptor linked signal transduction 2.17 P-signal transduction 1.56 F-receptor *F 6796 P-phosphate metabolism 7 0.957277 0.530667 0.0841395 FBgn0035854 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 7275 P-developmental processes 49 0.955749 0.563213 0.0990832 FBgn0017581 87.94 P-phosphate metabolism 0.01 P-phosphorylation 11.49 P-microtubule-based process *F 16021 C-integral membrane protein 7 0.955421 0.5517 0.0937562 FBgn0020429 2.61 F-cation channel 2.23 F-transmembrane receptor 1.37 C-plasma membrane/cell membrane *F 5886 C-plasma membrane/cell membrane 9 0.946714 0.558743 0.0981748 FBgn0039747 3.19 C-integral membrane protein 2.51 P-cell communication 2.31 F-transmembrane receptor *F 7275 P-developmental processes 50 0.945453 0.560307 0.0990832 FBgn0003302 3.71 P-cell growth and/or maintenance 1.45 F-molecular_function 0.76 P-phosphate metabolism *F 7275 P-developmental processes 51 0.945315 0.560268 0.0990832 FBgn0035800 10.45 P-microtubule-based process 0.29 P-cell organization and biogenesis 2.54 P-cytoplasm organization and biogenesis *F 5515 F-protein binding 26 0.942879 0.510927 0.0774462 FBgn0023177 0.1 P-phosphate metabolism 7.14 P-dephosphorylation 5.84 P-cytoskeleton organization and biogenesis *F 9605 P-response to external stimulus 9 0.941883 0.547898 0.0937562 FBgn0011268 6.32 F-G-protein coupled receptor/G-protein linked receptor 1.45 C-plasma membrane/cell membrane 1.31 F-receptor *F 5515 F-protein binding 27 0.934874 0.508722 0.0774462 FBgn0001085 3.8 P-cytoskeleton organization and biogenesis 2.33 P-cell organization and biogenesis 2.31 C-cell *F 5886 C-plasma membrane/cell membrane 10 0.928397 0.553546 0.0981748 FBgn0004242 5003.47 P-cell-cell signaling 17.18 P-cell communication 0.07 P-transmission of nerve impulse *F 5886 C-plasma membrane/cell membrane 11 0.928247 0.553503 0.0981748 FBgn0000119 4.26 F-transmembrane receptor 4.19 P-cell communication 0.4 P-signal transduction *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 38 0.92437 0.553833 0.0988553 FBgn0026603 9.83 C-nucleoplasm 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 6796 P-phosphate metabolism 8 0.924013 0.521413 0.0841395 FBgn0039172 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 6796 P-phosphate metabolism 9 0.924013 0.521413 0.0841395 FBgn0026567 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 6796 P-phosphate metabolism 10 0.924013 0.521413 0.0841395 FBgn0030306 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 9605 P-response to external stimulus 10 0.919794 0.541644 0.0937562 FBgn0028888 7.75 F-G-protein coupled receptor/G-protein linked receptor 1.36 F-receptor 0.82 C-cell *F 9605 P-response to external stimulus 11 0.919794 0.541644 0.0937562 FBgn0038379 7.75 F-G-protein coupled receptor/G-protein linked receptor 1.36 F-receptor 0.82 C-cell *F 9605 P-response to external stimulus 12 0.919794 0.541644 0.0937562 FBgn0030801 7.75 F-G-protein coupled receptor/G-protein linked receptor 1.36 F-receptor 0.82 C-cell *F 9605 P-response to external stimulus 13 0.919794 0.541644 0.0937562 FBgn0023524 7.75 F-G-protein coupled receptor/G-protein linked receptor 1.36 F-receptor 0.82 C-cell *F 9605 P-response to external stimulus 14 0.919794 0.541644 0.0937562 FBgn0039632 7.75 F-G-protein coupled receptor/G-protein linked receptor 1.36 F-receptor 0.82 C-cell *F 5886 C-plasma membrane/cell membrane 12 0.917691 0.550488 0.0981748 FBgn0004551 2.77 C-integral membrane protein 2.65 F-P-P-bond-hydrolyzis-driven transporter 0.65 F-ATP binding *F 5739 C-mitochondrion 9 0.916184 0.525477 0.086896 FBgn0000986 18.92 C-membrane 0.35 P-transport 1.75 P-metabolism *F 5515 F-protein binding 28 0.911585 0.502264 0.0774462 FBgn0026141 3.59 P-cytoskeleton organization and biogenesis 3.55 C-cytoskeleton 2.24 P-cell organization and biogenesis *F 7275 P-developmental processes 52 0.900929 0.547575 0.0990832 FBgn0013765 13.61 P-microtubule-based process 4.33 P-cell growth and/or maintenance 0.25 P-cell organization and biogenesis *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 27 0.898829 0.546491 0.0988553 FBgn0031254 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 27 0.898691 0.546451 0.0988553 FBgn0031254 12.79 F-GTP binding 0.41 F-guanyl nucleotide binding 2.4 F-purine nucleotide binding *F 5886 C-plasma membrane/cell membrane 13 0.897073 0.544554 0.0981748 FBgn0000382 19.2 P-cell communication 17.46 P-dephosphorylation 0.08 P-response to abiotic stimulus *F 6796 P-phosphate metabolism 11 0.895957 0.513499 0.0841395 FBgn0010348 18.11 P-protein modification 0.56 F-purine nucleotide binding 1.69 F-guanyl nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 28 0.890572 0.544098 0.0988553 FBgn0013969 10.24 F-GTP binding 0.44 F-guanyl nucleotide binding 2.15 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 28 0.890522 0.544083 0.0988553 FBgn0013969 10.24 F-GTP binding 0.44 F-guanyl nucleotide binding 2.15 F-purine nucleotide binding *F 7275 P-developmental processes 53 0.890066 0.544428 0.0990832 FBgn0020391 655.44 P-phosphate metabolism 0 P-phosphorylation 7.79 F-protein kinase *F 6796 P-phosphate metabolism 12 0.888895 0.511491 0.0841395 FBgn0023179 10.67 P-protein modification 0.83 F-peptidase 1.19 P-macromolecule biosynthesis *F 4888 F-transmembrane receptor 24 0.887356 0.537013 0.0959401 FBgn0011293 41.04 P-perception of external stimulus 0.04 P-perception of abiotic stimulus 0.18 P-sensory perception *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 39 0.885584 0.542648 0.0988553 FBgn0013753 9.83 C-nucleoplasm 0.72 C-nucleus 1.37 C-intracellular/protoplasm *F 16817 F-hydrolase, acting on acid anhydrides 29 0.88519 0.542533 0.0988553 FBgn0010391 10.24 F-GTP binding 0.44 F-guanyl nucleotide binding 2.15 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 29 0.884899 0.542448 0.0988553 FBgn0010391 10.24 F-GTP binding 0.44 F-guanyl nucleotide binding 2.15 F-purine nucleotide binding *F 16818 F-hydrolase, acting on acid anhydrides\, in phosphorus-containing anhydrides 30 0.884763 0.542408 0.0988553 FBgn0020655 10.24 F-GTP binding 0.44 F-guanyl nucleotide binding 2.15 F-purine nucleotide binding *F 16817 F-hydrolase, acting on acid anhydrides 30 0.884753 0.542405 0.0988553 FBgn0020655 10.24 F-GTP binding 0.44 F-guanyl nucleotide binding 2.15 F-purine nucleotide binding *F 7275 P-developmental processes 54 0.88266 0.542272 0.0990832 FBgn0035406 4.24 P-cell surface receptor linked signal transduction 3 P-cell communication 0.45 P-signal transduction *F 7275 P-developmental processes 55 0.881046 0.541801 0.0990832 FBgn0024327 5.35 P-cell surface receptor linked signal transduction 4.08 P-cell communication 0.31 P-response to abiotic stimulus *F 7275 P-developmental processes 56 0.876821 0.540567 0.0990832 FBgn0015296 9.27 P-cell surface receptor linked signal transduction 8.46 P-cell communication 0.21 P-response to abiotic stimulus *F 6796 P-phosphate metabolism 13 0.876564 0.507968 0.0841395 FBgn0030809 10.67 P-protein modification 1.19 P-macromolecule biosynthesis 0.85 P-protein biosynthesis/translation *F 9605 P-response to external stimulus 15 0.87414 0.528509 0.0937562 FBgn0020430 7.75 F-G-protein coupled receptor/G-protein linked receptor 1.36 F-receptor 0.8 C-integral membrane protein *F 7275 P-developmental processes 57 0.869064 0.538294 0.0990832 FBgn0002968 5.64 P-cell communication 1.61 C-plasma membrane/cell membrane 0.72 P-cell growth and/or maintenance *F 7275 P-developmental processes 58 0.867628 0.537872 0.0990832 FBgn0002945 4.24 P-cell surface receptor linked signal transduction 3 P-cell communication 0.45 P-signal transduction *F 4888 F-transmembrane receptor 25 0.866455 0.530924 0.0959401 FBgn0020440 10.15 F-protein kinase 2.51 P-phosphate metabolism 0.4 P-phosphorylation *F 5739 C-mitochondrion 10 0.864873 0.510809 0.086896 FBgn0019925 7.99 C-membrane 0.9 F-carrier/carrier type transporter 1.11 C-plasma membrane/cell membrane *F 6796 P-phosphate metabolism 14 0.861914 0.503756 0.0841395 FBgn0000115 18.11 P-protein modification 0.52 F-purine nucleotide binding 1.77 F-guanyl nucleotide binding *F 5198 F-structural protein 13 0.844093 0.500346 0.084918 FBgn0036882 8.68 C-microtubule cytoskeleton 3.7 P-cytoskeleton organization and biogenesis 0.28 P-microtubule-based process *F 5198 F-structural protein 14 0.844093 0.500346 0.084918 FBgn0021825 8.68 C-microtubule cytoskeleton 3.7 P-cytoskeleton organization and biogenesis 0.28 P-microtubule-based process *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 40 0.842521 0.529976 0.0988553 FBgn0026262 7.91 C-nucleoplasm 0.5 F-transcription factor 1.79 F-DNA binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 41 0.840215 0.529289 0.0988553 FBgn0011696 9.83 C-nucleoplasm 0.77 C-nucleus 1.22 C-intracellular/protoplasm *F 5386 F-carrier/carrier type transporter 11 0.823612 0.520571 0.097051 FBgn0036928 6.76 C-mitochondrial membrane 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 5386 F-carrier/carrier type transporter 12 0.823612 0.520571 0.097051 FBgn0010812 6.76 C-mitochondrial membrane 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 5386 F-carrier/carrier type transporter 13 0.823612 0.520571 0.097051 FBgn0037828 6.76 C-mitochondrial membrane 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 5386 F-carrier/carrier type transporter 14 0.823612 0.520571 0.097051 FBgn0016041 6.76 C-mitochondrial membrane 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 5386 F-carrier/carrier type transporter 15 0.823612 0.520571 0.097051 FBgn0033074 6.76 C-mitochondrial membrane 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 5386 F-carrier/carrier type transporter 16 0.823612 0.520571 0.097051 FBgn0032397 6.76 C-mitochondrial membrane 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 5886 C-plasma membrane/cell membrane 14 0.820105 0.521858 0.0981748 FBgn0015773 3.37 P-cell communication 2.2 P-embryogenesis and morphogenesis 1.56 C-extracellular *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 42 0.81973 0.523156 0.0988553 FBgn0038746 7.88 P-cytoplasm organization and biogenesis 1.6 C-intracellular/protoplasm 0.66 C-nucleus *F 7275 P-developmental processes 59 0.819262 0.523486 0.0990832 FBgn0022800 503.02 P-phosphate metabolism 0 P-phosphorylation 6.19 F-protein kinase *F 5386 F-carrier/carrier type transporter 17 0.815835 0.518235 0.097051 FBgn0023549 7.38 F-ion transporter 0.9 P-phosphate metabolism 1.08 P-phosphorylation *F 16021 C-integral membrane protein 8 0.812396 0.510268 0.0937562 FBgn0001263 0.02 P-perception of abiotic stimulus 34.55 P-perception of external stimulus 3.64 C-intracellular/protoplasm *F 7275 P-developmental processes 60 0.811587 0.52117 0.0990832 FBgn0004657 13.15 P-cell communication 0.34 C-integral plasma membrane protein 2.39 C-plasma membrane/cell membrane *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 43 0.807125 0.519349 0.0988553 FBgn0027375 5.04 F-DNA binding 1.82 F-nucleic acid binding 0.72 F-ATP binding *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 44 0.802006 0.517796 0.0988553 FBgn0021995 6.35 P-cytoplasm organization and biogenesis 1.42 F-nucleic acid binding 0.78 F-ATP binding *F 7275 P-developmental processes 61 0.797718 0.51696 0.0990832 FBgn0013272 5.35 P-cell surface receptor linked signal transduction 4.08 P-cell communication 0.31 P-response to abiotic stimulus *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 45 0.796837 0.516223 0.0988553 FBgn0040290 5.04 F-DNA binding 1.82 F-nucleic acid binding 0.67 F-ATP binding *F 16021 C-integral membrane protein 9 0.796159 0.505375 0.0937562 FBgn0024179 2.37 F-transmembrane receptor 1.77 P-phosphate metabolism 1.72 C-plasma membrane/cell membrane *F 5886 C-plasma membrane/cell membrane 15 0.795416 0.514386 0.0981748 FBgn0026379 15.97 P-dephosphorylation 0.08 P-phosphate metabolism 4.48 P-cell communication *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 46 0.792841 0.515004 0.0988553 FBgn0043001 7.91 C-nucleoplasm 0.8 C-nucleus 1.19 F-molecular_function *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 47 0.792841 0.515004 0.0988553 FBgn0043002 7.91 C-nucleoplasm 0.8 C-nucleus 1.19 F-molecular_function *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 48 0.792841 0.515004 0.0988553 FBgn0003175 7.91 C-nucleoplasm 0.8 C-nucleus 1.19 F-molecular_function *F 16021 C-integral membrane protein 10 0.790311 0.503602 0.0937562 FBgn0000464 2.13 F-transmembrane receptor 1.85 P-cell communication 1.72 C-plasma membrane/cell membrane *F 9605 P-response to external stimulus 16 0.787039 0.502607 0.0937562 FBgn0028961 0.01 P-G-protein coupled receptor protein signaling pathway 58.17 F-G-protein coupled receptor/G-protein linked receptor 3.59 P-cell surface receptor linked signal transduction *F 5386 F-carrier/carrier type transporter 18 0.786044 0.509199 0.097051 FBgn0030620 11.51 C-mitochondrial membrane 0.72 P-organelle organization and biogenesis 1.15 C-intracellular/protoplasm *F 5886 C-plasma membrane/cell membrane 16 0.776673 0.508647 0.0981748 FBgn0014432 0.09 F-ion channel 3.14 F-transmembrane receptor 3.01 F-channel/pore class transporter *F 5886 C-plasma membrane/cell membrane 17 0.776673 0.508647 0.0981748 FBgn0031293 0.09 F-ion channel 3.14 F-transmembrane receptor 3.01 F-channel/pore class transporter *F 5886 C-plasma membrane/cell membrane 18 0.776673 0.508647 0.0981748 FBgn0038840 0.09 F-ion channel 3.14 F-transmembrane receptor 3.01 F-channel/pore class transporter *F 7275 P-developmental processes 62 0.772616 0.509261 0.0990832 FBgn0001085 4.85 P-cell communication 3.78 P-cell surface receptor linked signal transduction 0.28 P-cell organization and biogenesis *F 5886 C-plasma membrane/cell membrane 19 0.764519 0.504894 0.0981748 FBgn0004864 11.78 P-cell communication 6.22 P-phosphorylation 0.17 P-phosphate metabolism *F 7275 P-developmental processes 63 0.76088 0.505625 0.0990832 FBgn0000163 10.45 P-microtubule-based process 5.52 P-cell growth and/or maintenance 0.29 P-cell organization and biogenesis *F 7275 P-developmental processes 64 0.753464 0.503315 0.0990832 FBgn0014018 29.77 P-cell communication 0.07 P-stress response 7.91 P-cell growth and/or maintenance *F 6139 P-nucleobase, nucleoside\, nucleotide and nucleic acid metabolism 49 0.751316 0.502179 0.0988553 FBgn0010278 3.33 F-DNA binding 1.9 F-nucleic acid binding 1.46 C-intracellular/protoplasm # *U FBrf0146769 *a Hickey *b D. *t 2002.3.11 *T personal communication to FlyBase *u *F > From dhickey@uottawa.ca Tue Mar 05 15:38:28 2002 *F > To: 'Haobo Jiang/ento/dasnr/Okstate' *F > Subject: Re: concerted evolution of a trypsin gene cluster *F > Cc: flybase-help@morgan.harvard.edu *F > *F > *F > Dear Haobo Jiang, *F > *F > Thank you for your message about Drosophila trypsin gene sequences. In *F > addition to the published paper that you referred to, we have continued to *F > work on this cluster and we have submitted several sequences to GenBank. *F > The sequence that would probably be most useful to you can be found under *F > accession number U04853 ( gi:11500040 ). As you will see, there are a *F > total of eleven trypsin-like sequences in this region; they are: *F > *F > LambdaTry (406-1224) GI : 11231181 *F > KappaTry complement (1439-2242) GI : 11231182 *F > ZetaTry complement(2802-3644) GI : 468845 *F > EtaTry complement (4180-4968) GI : 468844 *F > ThetaTry complement (5110-5898) GI : 468843 *F > AlphaTry complement (7436-8206) GI : 468842 *F > EpsilonTry complement (8391-9161) GI : 468841 *F > BetaTry (9449-10210) GI: 468840 *F > GammaTry complement (11888-12649) GI : 468839 *F > DeltaTry (13625-14386) GI : 468838 *F > IotaTry (15035-15793) GI : 1134972 *F > *F > Initially, there were several differences between our sequence and the *F > draft sequence of the complete Drosophila genome, but many of these *F > differences have been eliminated as the revisions of the complete genome *F > sequnce continues. One difference that remains is the one you noted, i.e., *F > we found eleven genes in this region whereas there are only ten genes *F > reported in the completed genome. This is due to the fact that two of the *F > genes (gamma and delta) have almost identical coding sequences and are *F > adjacent on the chromosome. This makes automated sequence assembly very *F > difficult. In fact we had to sub-clone the two coding regions into separate *F > plasmids before we could obtain the sequence. The computer assembly of the *F > scaffold sequences has combined the gamma and delta sequences, named it *F > 'gamma' and indicated the orientation of delta \- which is opposite to that *F > of gamma. This is why you get the impression that gamma is in the opposite *F > direction. *F . *F . *F > *F > I hope this information will be helpful to you. *F > *F > Best Regards, *F > *F > Donal Hickey. # *U FBrf0146770 *a Stevaux *b O. *t 2002.3.12 *T personal communication to FlyBase *u *F From stevaux@helix.mgh.harvard.edu Tue Mar 12 00:00:45 2002 *F To: Rachel Drysdale (Genetics) , *F Kevin Cook *F Subject: Re: Are you interested? *F Dear Rachel, *F Here is a detailed description of deletions in the CG4287 and CG5516 genes. *F The region is ordered as follow : *F \-----CG4287----CG5516----------RBF2--moira---P2000---- *F P2000 is described in a previous communication about RBF2. P2000 is *F male sterile at 25ºC but fertile at 18ºC and is inserted roughly *F 600bp upstream of moira. *F I have hopped P2000 and created the following chromosome: *F \-----CG4287---P16C3--CG5516----------RBF2--moira---P2000---- *F This new insertion P16C3 is viable and fertile at 18ºC, 16C3 is *F inserted exactly upstream of CG5516 (which doesn't have ESTs, so it *F is debatable if it is a real gene) and 200 bp upstream of the *F transcriptional start site of CG4287 (which does have an EST). *F The line containing P16C3 and P2000 was crossed to the transposase *F and precise deletions of the region between the 2 P-elements were *F recovered. The deletions take out moira, RBF2, CG5516, and *F potentially, bits of the CG4287 promoter as will become evident *F later. The ends of the two P-elements are still intact and one *F mini-white is still present in this background (from eye color and *F PCR data). *F \-----CG4287---P16C3--CG5516----------RBF2--moira---P2000---- *F \-----CG4287---[ 16C3 deletion *F ]---- (16C3 deletion, embryonic lethal) *F This deletion is embryonic lethal. It is lethal over several moira alleles. *F Genomic rescue constructs for moira were re-introduced. The *F phenotype turned into a larval lethality. This deletion with moira in *F the background complemented several null moira alleles Mor6 and Mor7. *F \-----CG4287---[ 16C3 deletion *F ]-------moira rescue--- (larval lethal) *F A genomic rescue construct covering RBF2, CG5516 and which cuts into *F CG4287 DOES NOT rescue the larval lethality of the deletion with the *F Moira rescue construct. However, a genomic rescue construct covering *F CG4287 and CG5516 and which cuts into RBF2 RESCUES the larval *F lethality. Hence, the larval lethality is due to the CG4287 gene. *F The phenotype is as follow: small larvae (2nd or early 3rd instar) *F crawl up the walls as if trying to pupate, but instead, they slow *F down and die, turning black. Two stable stocks are available. One *F has the 16C3 deletion, and the moira rescue construct and the other *F has the 16C3 deletion and the RBF2, CG5516 genomic rescue construct. *F When crossed to each other, the progeny displays the larval lethal *F phenotype and specifically removes CG4287. If CG5516 is indeed a *F gene, our 16C3 insertion P-element may inactivate it as it is *F inserted within 30bp upstream of the predicted ATG, but that *F insertion does not have a visible phenotype. We recovered three EMS *F alleles that are lethal over the 16C3 mutation but that are viable *F over two null moira alleles and are not mutated in RBF2 by *F sequencing. However, the lethality is no longer larval and some *F escapers occur when the EMS alleles are crossed to each other. These *F might be EMS mutants of CG4287 called alleles F, K and R. *F Best wishes, *F Olivier Stevaux *F \-- # *U FBrf0146771 *a Delidakis *b C. *c N. *d Giagtzoglou *t 2002.3.12 *T personal communication to FlyBase *u *F From delidaki@imbb.forth.gr Tue Mar 12 12:42:10 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: mapping of Gal4 line *F Hello *F we were interested to map the Gal4-C253 line, so my student (Nikolaos *F Giagtzoglou) did plasmid rescue and sequencing. The sequence we *F obtained is below. By BLAST we found that the transposon has inserted *F 153bp upstream of the scabrous gene. This agrees very well with the *F proneural cluster expression pattern that this driver gives. I'm *F sending this information to include in FLyBase, as it will probably be *F of use to the Drosophila community. *F Regards *F Christos *F CCCCCGCCCGGGGATCAGATCCGCGGCCGGCCGCATAGGCCACTAGTGGATCTGGATCCTCTAG *F CTAG|AGCTTTGCGTACTCGCAAATTATTAAAAATAAAACTTTAAAAATAATTTCGTCTAATTAA *F TATTATGAGTTAATTCAAACCCCACGGACATGCTAAGGGTTAATCAACAATCATATCGCTGTCT *F CACTCAGACTCAATACGACACTCAGAATACTATTCCTTTCACTCGCACTTATTGCAAGCATACG *F TTAAGTGGATGTCTCTTGCCGACGGGACCACCTTATGTTATTTCATCATGA|GCGGGGGAATTGC *F CGAGGAAGAGTGCCAAAGAGAGCAGCCACCTGCCCGAAGCATGCAACAAGCAGGGAAGAGAGCC *F ACAAAGAGAACGCATCAAGTTAAGAGAAAATCAATGAATAAACTCGATGTTAGATGCCCGAAAA *F ATGTCCTTTGAAATGTTTGCTATTACCAAAGATCATGTCTTTAAGGCAGCAGTTACCATTCGGA *F GTTTCAGATAGCGAACGAGAGCAACACAAAATGGCGGTCTAGTTACCCGGTGCTTCTTTTTTGC *F ACTCATCCTGCTCCTGACACATGGGCGCGGTTGCGGTGAGACTACAACGAAAACAAACTGCAAC *F GAAAACGGCAGATGTTCGGGCCCATAGGCAGGCAGGCAGACAGAAGGCGAAGTGCGAAAACATG *F CTGATGTGCTAGATATACTGCAAGACGAACAAAAACCAGACGG|GGCCCAAATGGTCCCAGTCCG *F GTAGTTGCAAGG *F To first | : plasmid DNA *F Between |1 and |2: P element *F Between |2 and |3: genomic DNA *F After |3: bad quality sequence # *U FBrf0146772 *a Cadigan *b K. *c K. *d Basler *e M. *f Bienz *t 2002.3.13 *T personal communication to FlyBase *u *F >From cadigan@umich.edu Wed Mar 13 05:13:04 2002 *F To: Rachel Drysdale *F Subject: Re: gammy legs *F Dear Rachel, *F Our paper describing the gammy legs gene is now officially in press. *F However, it is now called pygopus. It turns out that three labs (mine, *F Mariann Bienz's and Konrad Basler's) all discovered the gene independently *F and all have papers coming out in the next few months. We agreed to let a *F neutral party select a name-- so pygopus it is. The field is well served *F by this, though we still miss the old name. *F How do we go about changing the lead name. Is this email enough? *F Please let me know. *F Ken *F From rd120@gen.cam.ac.uk Wed Mar 13 12:04:08 2002 *F To: cadigan@umich.edu *F Subject: Re: gammy legs *F Hi Ken, *F Thankyou for that, and now FlyBase-Cambrige knows what a pygopus is (so *F well informed). *F I'm happy to rename based on what you say below. I'll also mail *F Mariann and Konrad to check whether or not their versions have sneaked *F out through an abstract somewhere (in which case I'll need to merge *F gene records), then I'll make a joint pc from you all. Congratulations *F on working out a nomeclature solution. Very pro-active. *F best regards, *F Rachel. *F From rd120@gen.cam.ac.uk Wed Mar 13 13:17:43 2002 *F To: basler@molbio.unizh.ch, mb2@mrc-lmb.cam.ac.uk, cadigan@umich.edu *F Subject: Re: gammy legs *F Dear Mariann and Konrad (and Ken, see the bit about the symbol which I *F forgot to mention in my last), *F I have this mail below from Ken and will change gammy legs to pygopus. *F I'm writing to ask whether Mariann and/or Konrad have published *F abstracts or sequence accession records about this gene under another *F name/symbol. If so, then there is a chance (abstracts) or certainty *F (sequence accession records) that we already have a record in FlyBase. *F I will take this opportunity to merge the records, though it will take *F weeks for the merge to show up on the public server (just missed the *F cut-off for a round of updates). *F I'm not sure what you plan for the short symbol. It can't be pyg as we *F already have a pyg: pygoscelis (FBgn0020790), and it seems to me that *F to choose pygo might be asking for trouble with respect to potential *F for confusion with pygoscelis. How about pygp? *F Thankyou for your help, *F Best wishes, *F Rachel. *F From basler@molbio.unizh.ch Wed Mar 13 14:17:19 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: gammy legs *F Dear Rachel, *F thanks for your help in this matter. I have only one comment to add: *F the abbreviation must be pygo. For several reasons: 1) it's always *F better if it can be pronounced, 2) we can no longer change it in our *F manuscript which is in press, 3) Mariann also used pygo as the *F abbreviation in her paper, and finally, I don't worry about possible *F confusion with pygoscelis, these proteins act in different systems *F and are unlikely to ever be used in the same context. *F Hope you will agree.. *F Best wishes, *F Konrad *F From rd120@gen.cam.ac.uk Wed Mar 13 14:21:05 2002 *F To: basler@molbio.unizh.ch, mb2@mrc-lmb.cam.ac.uk, cadigan@umich.edu *F Subject: Re: gammy legs *F Hi Konrad, *F all your reasons are sound but reason number 2 particularly *F compelling! *F I'm happy for it to be pygo if you all are. *F Rachel. *F From mb2@mrc-lmb.cam.ac.uk Wed Mar 13 14:39:21 2002 *F To: Rachel Drysdale *F Subject: Re: gammy legs *F I completely agree with Konrad, Rachel \- it must be pygo! *F It was quite an effort finding this name (and Roel Nusse gets the credit *F for finding it, and getting us to agree), and I think we've done well to *F end up with one rather than three names. *F Cheers, Mariann # *U FBrf0146773 *a Carpenter *b A.T.C. *t 1993.8.8 *T personal communication to FlyBase *u *F > From ma11@gen.cam.ac.uk Sat Aug 7 17:50:49 1993 *F > From: Michael Ashburner (Genetics) *F > Date: Sat, 7 Aug 93 17:54:05 BST *F > To: ag24@gen.cam.ac.uk *F > Subject: Prl *F > Cc: m.ashburner@gen.cam.ac.uk *F > Content-Length: 643 *F > Status: RO *F > X-Lines: 19 *F > *F > Adelaide just pointed out that there is no entry in the RedBook for *F > Prl, referred to under Df(2L)Prl. *F > I guess I had better invent one to go into genes93 *F > *F > \*a Prl *F > \*e Proxless *F > \*c 32F1-3;33F1-2 based on the limits of Df(2L)Prl. *F > \*b 2-44-45 *F > \*o Associated with Df(2L)Prl *F > \*w B.S. Baker *F > \*x B.S. Baker, in Lindsley and Zimm (1992) *F > \*p A dominant phenotype associated with Df(2L)Prl. The anterior and posterior *F > \*p crossveins, as well as the L5 vein are missing; the L2 is either *F incomplete *F > \*p or absent. The wings may appear to be somewhat shortened. *F > \# *F > *F > *F > Put this in, though Adelaide has been asked by me to check this \! \- since *F > she is now working with it. *F > *F > From ATC12@phx.cam.ac.uk Sun Aug 8 14:33:39 1993 *F > Date: Sun, 08 Aug 93 14:31:46 BST *F > From: ATC12@phx.cam.ac.uk *F > To: ag24@phx.cam.ac.uk *F > Cc: ma11@phx.cam.ac.uk *F > Subject: Prl *F > Content-Length: 467 *F > Status: RO *F > X-Lines: 11 *F > *F > In the meantime I've taken a closer look; the previous *F > description was from a quickie \-- substitute the following *F > *F > Proximal region of wing missing, including costal cell, anal *F > crossvein and vein L6; alula present but approximately *F > half normal length. Anterior crossvein present but much *F > more proximal than normal. In addition, vein L5 and *F > posterior crossvein missing and vein L2 incomplete at *F > distal end. Wing shorter than normal and tends to be *F > slightly wavy. *F > *F > *F > From ma11@gen.cam.ac.uk Sun Aug 8 15:11:37 1993 *F > From: Michael Ashburner (Genetics) *F > Date: Sun, 8 Aug 93 15:14:52 BST *F > To: ag24@gen.cam.ac.uk *F > Subject: Prl revised *F > Content-Length: 567 *F > Status: RO *F > X-Lines: 17 *F > *F > As revised by Adelaide *F > *F > \*a Prl *F > \*e Proxless *F > \*c 32F1-3;33F1-2 based on the limits of Df(2L)Prl. *F > \*b 2-44-45 *F > \*o Associated with Df(2L)Prl *F > \*w B.S. Baker *F > \*x B.S. Baker, in Lindsley and Zimm (1992) *F > \*p Proximal region of wing missing, including costal cell, anal *F > \*p crossvein and vein L6; alula present but approximately *F > \*p half normal length. Anterior crossvein present but much *F > \*p more proximal than normal. In addition, vein L5 and *F > \*p posterior crossvein missing and vein L2 incomplete at *F > \*p distal end. Wing shorter than normal and tends to be *F > \*p slightly wavy. *F > \# # *U FBrf0146774 *a Alonso *b C. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:32:45 2002 *F To: cra21@hermes.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10634 *F Dear Claudio, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila development under surveillance?' *F You mention a gene that is new to FlyBase, Upf1. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From cra21@hermes.cam.ac.uk Mon Mar 18 10:24:11 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10634 *F Dear Rachel, *F Many thanks for your message. The gene we describe in our abstract as *F Drosophila melanogaster's Upf1 or Dm-Upf1, corresponds to the Genome *F Project annotation CG1559. *F We are now in the process of submitting a manuscript describing our *F findings on Drosophila NMD etc, so I would appreciate you could let me know *F what is the format in which you would like us to cite CG1559 as a reference *F of the Genome project. *F Yours ever, *F Claudio *F ________________________________________________ *F Dr. Claudio Alonso *F Research Associate *F Department of Zoology *F University of Cambridge *F Downing Street *F Cambridge CB2 3EJ *F United Kingdom. *F e-mail: cra21@hermes.cam.ac.uk *F Tels: \+44 \- 1223 \- 331772 *F Fax: \+44 \- 1223 \- 336679 *F ________________________________________________ # *U FBrf0146775 *a Vass *b S. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:32:12 2002 *F To: S.Vass@ed.ac.uk *F Subject: Helping FlyBase: ADRC-10630 *F Dear Sharron, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Depletion of the cohesin subunit Drad21/Scc1 in Drosophila cultured cells *F results in premature sister chromatid separation and a prometaphase delay.' *F You mention a gene that is new to FlyBase, SMC3. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From S.Vass@ed.ac.uk Mon Mar 18 11:08:04 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10630 *F Dear Rachel, *F thank you for the inquiry regarding the Drosophila SMC3 gene. *F The cg number for this gene is 9802, and here it is called 'cap' or *F chromosome associated protein, but it is also the fly homologue of *F the SMC3 gene. I hope this information is helpful to you. *F kind regards, *F Sharron Vass. *F Sharron Vass *F The Wellcome Trust Centre for Cell Biology *F Institute of Cell and Molecular Biology *F University of Edinburgh *F Michael Swann Building, King's Buildings, *F Mayfield Road, Edinburgh. EH9 3JR. UK *F Tel: \+44 (0) 131 650 7109 # *U FBrf0146776 *a Moses *b K. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:50:51 2002 *F To: kmoses@cellbio.emory.edu *F Subject: Helping FlyBase: ADRC-10378 and 10369 *F Dear Kevin, *F Congrats! your computer is plugged in again. What is more it is that *F time of the year when I get to bug you about your abstracts! *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Mkph, where the Notch pathway meets Egfr pathway in Drosophila eye *F development.' *F and *F 'Egfr, Notch and MAP kinase in the developing eye: a tale of two signals.' *F You mention a gene called Mkph in the first, and I'm wondering if it *F the same Mkph that we already know about \- FBgn0041200. If not, or if *F you cannot say, then I should make a separate gene record for it. You *F may know which CG it corresponds to \- and if you could tell me which it *F is then that would be great. All the CGs have corresponding gene *F records in FlyBase already and we don't like to make duplicate records *F for what is actually the same gene unless we can't avoid it. *F The second abstract discusses a MAP kinase phosphatase you refer to as *F Mkp1 and Mkp-1. It seem very likely that this is exactly the same gene *F as Mkph and you are going to have to settle on a single symbol for it. *F If you are not sure about the identity of your Mkph with our Mkph then *F might I suggest that you choose to go with either Mkp1 or Mkp-1? *F Please advise!! *F Thank you very much for your help, *F all the best, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kmoses@cellbio.emory.edu Mon Mar 18 13:46:32 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10378 and 10369 *F Mkph is the one you know and love. Mkp1 is a human homolog. *F Note: Mkph is identical by DNA sequence to slingshot (ssh), both of *F which are separately listed in polytene region 96B on FlyBase! *F They are in different places on Gadfly! *F Amazing! *F \-- *F Regards, Kevin *F Kevin Moses *F Department of Cell Biology *F Emory University School of Medicine *F 615 Michael Street *F Atlanta, GA 30322-3030 *F (404) 727-8799 \- phone (office) *F (404) 727-4815 \- phone (lab) *F (404) 727-4717 \- fax *F kmoses@cellbio.emory.edu *F http://www.emory.edu/CELLBIO/moses/ # *U FBrf0146777 *a Manheim *b E. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:48:40 2002 *F To: emanheim@waksman.rutgers.edu *F Subject: Helping FlyBase: ADRC-10317 *F Dear Elizabeth, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'C(2)M is a component of the synaptonemal complex specifically required for *F crossing over.' *F You mention a gene that is new to FlyBase, c(2)M. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From emanheim@waksman.rutgers.edu Mon Mar 18 13:51:46 2002 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10317 *F Hi Rachel--C(2)M is actually the same thing as mei-910, which is already *F in flybase. The original name arose from a screen we did, but we have *F decided to change the name to c(2)M so that it is more in keeping with its *F function and the other known protein, c(3)G, that it is most *F similar to. c(2)M is also a crossover *F suppressor, on second instead of third, and is a synaptonemal complex *F component as well. Thus, we thought it would be kind of clever and in *F keeping with c(3)G to name it c(2)M. *F Is this helpful information? Do you need anything more? *F Also, mei-910 is listed under GM03132 as well. *F Elizabeth # *U FBrf0146778 *a Behr *b M. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:47:19 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 18 Mar 2002 09:47:19 \+0000 *F To: mbehr@gwdg.de *F Subject: Helping FlyBase: ADRC-10282 *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 18 Mar 2002 09:47:17 \+0000 *F Content-Length: 1529 *F Dear Matthias, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Branch morphogenesis of the tracheal system.' *F You mention a gene that is new to FlyBase, wus. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Also, you say the defining lethal mutation, which affects tracheal *F development in the embryo, came from the Gottingen collection. We have *F records for the Gottingen insertions already in FlyBase. If you let me *F know which particular insertion it is that defines wus then I can *F relate wus to that insertion and keep the various bits of data *F correctly inter-related in FlyBase. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From mbehr@gwdg.de Mon Mar 18 14:24:35 2002 *F To: rd120@gen.cam.ac.uk *F Subject: wurst identification *F Dear Rachel, *F the P-Insertion, which affects the wurst (wus) gene is, named l(1)G0162 *F (PlacW) generated by the Göttingen X-project (U.Schaefer). The wurst *F gene correspond to CG9089. *F If you have additional questions or something is unclear, please ask again, *F best wishes, *F Matthias Behr *F \------------------- *F Matthias Behr *F Max Planck Institut fuer biophysikalische Chemie *F Abt. 170; Molekulare Entwicklungsbiologie *F Am Fassberg 11 *F 37077 Goettingen *F Germany *F Tel.: \++(0)551/2011660 *F Fax: \++(0)551/2011755 *F Mail: mbehr@gwdg.de *F \------------------- # *U FBrf0146779 *a Minakhina *b S. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:33:57 2002 *F To: svetlana@waksman.rutgers.edu *F Subject: Helping FlyBase: ADRC-10683 *F Dear Svetlana, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A novel protein that monitors the level of Dorsal nuclear import.' *F You mention a gene that is new to FlyBase, tamo. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From svetlana@waksman.rutgers.edu Mon Mar 18 14:51:43 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10683 *F tamo is CG4057, we also called it 60B as cytogene (this name we used *F provisionally on 41st *F Annual Drosophila Research Conferences) # *U FBrf0146780 *a Gao *b Z. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:56:18 2002 *F To: zgao@ms.soph.uab.edu *F Subject: Helping FlyBase: ADRC-10445 *F Dear Zhiqian, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Molecular Genetic Characterization of Fly PKD2 gene.' *F You mention a gene, Pkd2. Do you know which of the Genome Project CG *F annotations your gene corresponds to? The text of your abstract *F indicates that you do. All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From ZGao@ms.soph.uab.edu Mon Mar 18 15:03:35 2002 *F To: 'Rachel Drysdale' *F Subject: RE: Helping FlyBase: 10445 *F Hi, *F It's CG6504. Do I need to update the information of CG6504 in the database? *F There is a little difference with the cloned gene. *F Thanks *F Zhiqian # *U FBrf0146781 *a Tavsanli *b B. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:49:40 2002 *F To: bt691998@bcm.tmc.edu *F Subject: Helping FlyBase: ADRC-10368 *F Dear Beril, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Functional Analysis of the Bestrophin Gene Family in Drosophila.' *F You mention four genes that are new to FlyBase, Best1, Best2, Best3 and *F Best4. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your genes do not *F correspond to CGs then perhaps you could tell me their map location, as *F this is valuable information for the genome annotation project. *F As it happens we already have a gene record for 'best' (FBgn0040238) *F resulting from your sequence accession record AF218817, and this is *F linked to CG6264. Would the other three be CG7259, CG10173 and *F CG12327? If so please let me know the correspondences of Best1, 2, 3 *F and 4 to these CGs. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From bt691998@bcm.tmc.edu Mon Mar 18 15:22:22 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10368 *F Dear Rachel, *F Thank you for your e-mail. You are right about the CGs mentioned. The way *F we named the dbest family was dbest1 = CG6264; dbest2 = CG10173; dbest3 = *F CG12327 and dbest4 = CG7259. I would be happy to modify the abstract to *F include the CGs if you think it is necessary. Please let me know if there *F is anything else I can help you with. *F Best regards, *F Beril. *F Beril Tavsanli *F Mardon Lab *F Baylor College of Medicine *F Department of Pathology, S242 *F One Baylor Plaza *F Houston, TX 77030 *F Phone: (713) 798-8715 *F e-mail: bt691998@bcm.tmc.edu # *U FBrf0146782 *a Nilson *b L. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:45:03 2002 *F To: Laura.Nilson@mcgill.ca *F Subject: Helping FlyBase: ADRC-10160 *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 18 Mar 2002 09:45:01 \+0000 *F Content-Length: 1227 *F Dear Caroline, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Phenotypic characterization of F72: a novel mutation affecting epithelial *F organization.' *F You mention a gene, F72. Do you know which of the Genome Project CG *F annotations F72 corresponds to yet? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F If your gene does not correspond to a CG then perhaps you could tell me *F its map location, as this is valuable information for the genome *F annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Laura.Nilson@mcgill.ca Mon Mar 18 15:27:36 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10160 *F Dear Rachel, *F F72 is a mutation isolated in an EMS screen. Using standard meiotic *F recombination we have found that it maps just proximal to dumpy. We *F are currently beginning SNP mapping to further map it's location, but *F we have no idea yet which gene is affected by this mutation. *F I hope this information is helpful. Let me know if you have any *F other questions. *F Best wishes, *F Laura Nilson *F ___________________________________ *F Laura Nilson, Ph.D. *F Assistant Professor *F Canada Research Chair in Genetics *F Department of Biology *F McGill University *F 1205, avenue Docteur Penfield *F Montreal QC H3A 1B1 Canada *F phone: 514-398-6448 *F fax: 514-398-5069 *F ___________________________________ # *U FBrf0146783 *a Finley *b K. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:54:10 2002 *F To: finley@salk.edu *F Subject: Helping FlyBase: ADRC-10421 *F Dear Kim, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Drosophila blue cheese Gene Identifies a Novel Progressive Neural *F Degenerative Pathway.' *F You mention a gene that is new to FlyBase, bchs. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From finley@salk.edu Tue Mar 19 00:43:58 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10421 *F >Dear Rachel, *F I'm sorry for the confusion. There is a Flybase record for a portion *F of the blue cheese gene noted as CG9011, map location 26A1. This is *F a very long story but part of the confusion comes from our difficulty *F in publishing our results on the entire gene, gene family and mutant *F phenotype. The the beach1 gene as characterized in Flybase is *F missing 5' and 3' exons making it an incomplete message. Added to *F the carboxy terminus of BCHS is a motif that makes it distinct from *F others containing beach domains (i.e.lyst). We have identified other *F BCHS proteins from worms to humans making this a unique protein *F sub-family. *F The blue cheese name characterizes the mutant phenotype in which *F there is progressive development of protein inclusions throughout the *F adult CNS. We were following the swiss cheese example of naming *F neurodegeneration mutants (i.e. progressive development of vacuoles *F or holes in the adult CNS). We have four independent mutant alleles *F (EP2299 included) all showing different degrees of the phenotype. *F ... I hope this clarifies everything.... *F Kim Finley # *U FBrf0146784 *a Wu *b V. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:22:15 2002 *F To: circe@northwestern.edu *F Subject: Helping FlyBase: ADRC-10435 *F Dear Victoria, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Molecular and genetic analysis of sinuous, a gene that controls *F tracheal tube size.' *F Your abstract indicates that you now know the molecular nature of sinu *F \- hence you will know which CG gene annotation it corresponds to. If *F you could let us have that information I can link the two gene records *F (sinu and CG) in FlyBase which would be far better than having two distinct *F entries for what is actually the same gene. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From circe@nwu.edu Mon Mar 18 16:50:47 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10435 *F Hi Racheal, *F The CG that sinuous corresponds to is CG10624. I hope that helps, if *F you need anymore information please let me know and I will be happy *F to help. Thanks. *F Victoria # *U FBrf0146785 *a Beller *b M. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:29:10 2002 *F To: mbeller@gwdg.de *F Subject: Helping FlyBase: ADRC-10529 *F Dear Mathias, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Ima, a novel partner of dRalA, interacts with Smox and RNA-binding *F proteins.' *F You mention a gene that is new to FlyBase, Ima. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From mbeller@gwdg.de Mon Mar 18 17:22:19 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10529 *F Dear Rachel, *F The gene we call ima consists of the two annotated sequences CG4117 and *F CG15656. In the course of our studies we sequenced the EST clone LD27118 *F and found that its sequence combines both predicted genes. The predicted *F domain structure of CG4117 remains unaffected. *F With best wishes, *F Mathias Beller *F Mathias Beller *F Max-Planck-Institut für biophysikalische Chemie *F Abteilung Molekulare Entwicklungsbiologie *F Am Fassberg 11 *F 37077 Göttingen *F Telefon: 0551 / 2011657 # *U FBrf0146786 *a Chen *b H. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:43:56 2002 *F To: chenhw@mail.ncifcrf.gov *F Subject: Helping FlyBase: ADRC-11010 *F Dear Hua-Wei, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'mom identifies a receptor for the Drosophila JAK/STAT signal transduction *F pathway and encodes a protein distantly related to the mammalian cytokine *F receptor family.' *F You mention a gene that is new to FlyBase, mom. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F We noticed that your group has recently published a paper describing a *F receptor for the JAK/STAT pathway: *F \*x FBrf0141438 == Brown et al., 2001, Curr. Biol. 11(21): 1700--1705 *F Is mom by any chance the same gene as dome? *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From chenhw@mail.ncifcrf.gov Mon Mar 18 17:34:42 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-11010 *F Dear Rachel, *F The mom is the same gene as dome. *F Best wishes. *F Hua-Wei # *U FBrf0146787 *a Ketel *b C. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:46:07 2002 *F To: kete0013@tc.umn.edu *F Subject: Helping FlyBase: ADRC-10204 *F Dear Carrie, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of Drosophila MES-4.' *F You mention a gene that is new to FlyBase, Mes-4. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kete0013@tc.umn.edu Mon Mar 18 18:04:25 2002 *F From: 'Carrie Ketel' *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10204 *F Dear Rachel, *F dMes-4 corresponds to CG4976. We have called it dMes-4 because of homology *F to the C. elegans Mes-4 (in Wormbase) described by Susan Strome's lab at *F Indiana. *F Thanks, *F Carrie # *U FBrf0146788 *a Reeves *b N. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:25:11 2002 *F To: nreeves@ucsd.edu *F Subject: Helping FlyBase: ADRC-10473 *F Dear Nick, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analysis of a KCNQ potassium channel gene in Drosophila.' *F You mention a gene that is new to FlyBase, KCNQ. Do you know which of *F the Genome Project CG annotations your gene corresponds to? Is it by *F any chance CG12215? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From nreeves@popmail.ucsd.edu Mon Mar 18 19:01:56 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10473 *F Rachel, *F Yes the KCNQ gene I'm referring to does have a corresponding *F CG number (well actually 2). I have isolated three full length cDNA's *F (each a different splice variant) and all three overlap with CG12215 *F and CG12915. The actual structure of the cDNA I've found is *F different from the gadfly reports too. If you want the cDNA sequences *F I can send them to you. *F I hope this helps, *F Nick # *U FBrf0146789 *a Page-McCaw *b A. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:30:05 2002 *F To: andrea@fruitfly.berkeley.edu *F Subject: Helping FlyBase: ADRC-10552 *F Dear Andrea, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Matrix metalloproteinase function in Drosophila development.' *F You mention a gene that is new to FlyBase, Mmp2. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From andrea@fruitfly.bdgp.berkeley.edu Mon Mar 18 22:00:52 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10552 *F Dear Rachel, *F You asked for a CG number that corresponds for the gene MMP2 I *F mention in my fly meeting abstract. The number is CG1794. Thank you. *F Best wishes, *F Andrea # *U FBrf0146790 *a Spencer *b S. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:36:15 2002 *F To: sspencer@pharmsun.wustl.edu *F Subject: Helping FlyBase: ADRC-10768 *F Dear Susan, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'ed and fred: inhibitors of EGF receptor signaling.' *F You mention a gene that is new to FlyBase, fred, and say that it lies *F just upstream of ed. Having checked upstream of ed in Geneseen I see *F nothing very much (though this depends on exactly what you mean by *F 'just upstream'). Could you confirm for me (or otherwise set me *F straight) that fred was missed in the genome annotation project? If it *F was I urge that you submit a sequence accession record for fred to *F Genbank so that it will then be drawn onto the genome annotation (there *F is a data path within FlyBase that will ensure that this is so), next *F time the annotation looks at that region of the chromosome. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From sspencer@pcg.wustl.edu Mon Mar 18 22:05:50 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10768 *F Dear Rachel, *F Thanks for your note. Several predicted genes (CG12675, CG3393, CG3390) *F match pieces of Fred, but none matches the 5' end of the protein. The 5' *F end of Fred is at 255,363 of AE003578; echinoid's 5' end is at 295,068, and *F they are divergently transcribed. I guess 40,000 bases away may not be *F 'just upstream', but then again, each of these genes spans more than 80,000 *F bases (with many introns, of course), so their 5' ends are relatively close *F together! *F I'll submit the fred sequence to genbank ASAP. Good luck with your annotation. *F Susan # *U FBrf0146791 *a Han *b C. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:42:45 2002 *F To: han_chun@hotmail.com *F Subject: Helping FlyBase: ADRC-10969 *F Dear Chun, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Functional analysis of EXT family of tumor suppressor genes in Drosophila.' *F You say 'tout-velu (botv) and sister of tout-velu (sotv), which encode *F the homologs of human EXTL3 and EXT2, respectively'. I am writing *F about sotv. The thing is that we already have a gene record for Ext2 *F (FBgn0029175) though there is very little data in it. There are two *F papers which have mentioned Ext2: *F \*x FBrf0128173 == Perrimon, 2000, Nature 404(6779): 725--728 *F \*x FBrf0123217 == The et al., 1999, Molec. Cell 4(4): 633--639 *F Is this gene the same as your sotv? If so I will enter your abstract as a *F reference for Ext2, but if not I will make a new gene record for sotv. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From han_chun@hotmail.com Mon Mar 18 23:58:52 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10969 *F Dear Rachel, *F I think we are talking about the same gene. sotv's BcDNA ID is GH02288. You *F can find this gene by searching with 'GH02288' in flybase. It is located at *F 2R 52F3-52F3. The two references flybase already has only said there is an *F Ext2 gene in Drosophila, however, they didn't mention exactly which gene is *F Ext2. In our work, we isolated mutant alleles of GH02288, which is the very *F Drosophila Ext2 gene. I hope the explanation clarified the issue. Thanks for *F asking. *F Best regards *F Chun # *U FBrf0146792 *a Lin *b Y.F. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:25:55 2002 *F To: ivlin99@hotmail.com *F Subject: Helping FlyBase: ADRC-10484 *F Dear Yi-Fan, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Analyzing the function of Nedd8 in Drosophila.' *F You mention a gene that is new to FlyBase, Nedd8. Do you know which of *F the Genome Project CG annotations your gene corresponds to? Is it by *F any chance CG10679? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From ivlin99@hotmail.com Tue Mar 19 03:50:43 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10484 *F Dear Rachel: *F Indeed, the Nedd8 gene we invastigated is CG10679 in *F annotated Genome Data Base. However, on going of the paper *F writing, we would rather to name the gene 'dNedd8'. *F Thanks for your correspondence and your work is *F really helpful. *F sincerely yours, *F Lin Yi-Fan. # *U FBrf0146793 *a Pai *b L.M. *t 2002.3.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 10:04:59 2002 *F To: gschupbach@molbiol.princeton.edu *F Subject: Helping FlyBase: ADRC-10239 *F Dear Trudy, *F I directed the following query to Li-Mei but her email address seems no *F longer to be valid. Please could you pass it on or (equally fine by *F me!) answer it yourself. *F With my best wishes, *F Rachel. *F \----- Begin Included Message \----- *F >From rd120@gen.cam.ac.uk Mon Mar 18 09:47:01 2002 *F Subject: Helping FlyBase: ADRC-10239 *F Dear Li-Mei, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Genes required for dorsoventral patterning in Drosophila follicle cells.' *F You mention a gene that is new to FlyBase, G192. Do you know yet which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From pai@mail.cgu.edu.tw Tue Mar 19 08:06:46 2002 *F To: *F Subject: RE: Helping FlyBase: ADRC-10239 *F Dear Rachel: *F I am writing about the mutant, G192, which was identified in my screen and *F we have not finished the mapping process. Meanwhile we only know it locates *F between hairy and thread, and have no information about corresponding *F annotations. Thank you. *F Best wishes, *F Li-Mei # *U FBrf0146794 *a Feix *b M. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:26:14 2002 *F To: mfeix@uni-bonn.de *F Subject: Helping FlyBase: ADRC-10490 *F Dear Maritta, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Verstopft (vsp) which encodes a novel single-pass membrane protein is *F required for epithelial development in Drosophila.' *F You mention a gene that is new to FlyBase, vsp. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From mfeix@uni-bonn.de Tue Mar 19 14:10:49 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10490 *F Dear Rachel, *F the gene we originally named verstopft (vsp) corresponds to CG14226. *F Just a few days after submission of our abstract for the 43rd Annual *F Drosophila Research Conference CG14226 was published as domeless by *F Brown et al. in Current Biology, 2001, 11 (21), 1700 \- 1705: *F Identification of the first invertebrate interleukin JAK/STAT receptor, *F the Drosophila gene domeless. *F If there are further questions please contact me, *F with best wishes, *F Maritta. *F \------------------------------- *F Dr. Maritta Feix *F University of Bonn *F Institute for Zoophysiology *F Dept. Developmental Biology *F Poppelsdorfer Schloss *F 53115 Bonn *F Germany *F Tel: 49 228-736326 *F Fax: 49 228-734480 *F E-mail: mfeix@uni-bonn.de # *U FBrf0146795 *a Checkland *b T. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:37:18 2002 *F To: tlc2@ualberta.ca *F Subject: Helping FlyBase: ADRC-10823 *F Dear Tamara, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila homologue of C. elegans unc-45: a novel myosin interacting *F protein.' *F You mention a gene that is new to FlyBase, unc-45. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F You say 'We have obtained a strain that carries a P-element insertion *F 500 base pairs upstream of this coding region'. Is this one of the *F BDGP insertions? If so we will already have a record for the insertion *F so please could you let me know which one it is, so that I can keep all *F the data properly inter-related. If this element is affecting unc-45 *F (your abstract suggests that it does) then I will make an allele of *F unc-45 to reflect that. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From tlc2@ualberta.ca Tue Mar 19 16:42:14 2002 *F To: 'Rachel Drysdale, Ph.D.' *F Subject: RE: Helping FlyBase: ADRC-10823 *F Rachel, *F the CG number is CG2708 (FlyBase ID: FBgn0010812) and the gene is *F currently called Tom 34, this implies a mitochondrial localization. We *F believe CG2708 is more likely to represent a homologue of Unc-45 in C.elegans. *F This is a cytoplasmicly localized protein involved in muscle formation in the *F nematode. We will be presenting evidence at the meeting. Sorry for any *F confusion. *F Tamara # *U FBrf0146796 *a Gindhart *b J. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:35:40 2002 *F To: joseph.gindhart@umb.edu *F Subject: Helping FlyBase: ADRC-10748 *F Dear Joseph, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification and characterization of YETI, a novel kinesin-I associated *F protein.' *F You mention a gene that is new to FlyBase, Yeti. A gene named yeti has *F been reported to FlyBase previously \- see the record for FBgn0016081 *F (the gene is now known as fry). I doubt very much whether the two *F yetis (!) are the same gene, but I need you to confirm that for me. *F Do you know which of the Genome Project CG annotations your Yeti *F corresponds to? (What you say about 'not yet been located within the *F Drosophila genome' does not rule this out, I think). All the CGs have *F corresponding gene records in FlyBase already and we don't like to make *F duplicate records for what is actually the same gene unless we can't *F avoid it. If your gene does not correspond to a CG then perhaps you *F could tell me its map location, as this is valuable information for the *F genome annotation project. *F Finally \- what is the derivation of the Yeti name? We record this for *F interestingly named genes (for fun!) *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Joseph.Gindhart@umb.edu Tue Mar 19 17:11:46 2002 *F To: 'Rachel Drysdale (Genetics)' , *F Joe Gindhart *F Subject: Re: Helping FlyBase: ADRC-10748 *F Dear Rachel, *F I am sure that YETI and furry are not the same gene. We pulled YETI out of a *F 2-hybrid *F screen of an embryonic cDNA library. It is encoded by EST GH01620, and the *F gene is *F within genomic clone AE003220. The YETI protein is 241aa, and appears to be *F the fly *F homolog of mammalian BCNT and cp27 (which have no known function). YETI does *F not have a *F CG annotation (I did a BLASTX and TBLASTX searches on fruitfly.org today). *F We do not yet know its map location (I am hoping the genome sequencing project *F will help *F :-) ). The YETI gene is flanked by transposable elements, which probably *F explains why it *F has not yet been assembled into the genomic sequence. *F We named the gene Yeti because ofits similarity to the abominable snowman. *F Yeti is in *F the fly genome, but we don't know exactly where. By analogy, the abominable *F snowman is *F in the Himalayas, but we don't know exactly where. We had also considered *F calling the *F gene Bigfoot, Nessie, Jersey Devil, or Skunk Ape, but Yeti was the most poetic. # *U FBrf0146797 *a Curtis *b D. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:38:44 2002 *F To: curtis@exelixis.com *F Subject: Helping FlyBase: ADRC-10907 *F Dear Daniel, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'New Genes Required for Presenilin Function.' *F You mention genes that are new to FlyBase, pen-1 and pen-2. Do you *F know which of the Genome Project CG annotations your genes correspond *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If your genes do not correspond to CGs *F then perhaps you could tell me their map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From curtis@exelixis.com Tue Mar 19 18:07:51 2002 *F Subject: Re: Helping FlyBase: ADRC-10907 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F Our gene pen-1 is CG2855. pen-1 is also recently identified in C. elegans *F and was called aph-1 (Goutte et al. 2002, PNAS 99:775). *F The Drosophila orthologue of the C. Elegans PEN-2 gene resides on *F Drosophila Melanogaster Chromosome 2R (55C1-55C5) between the Ote (Otefin \- *F CG5581) and sbb (CG5580) genes. There are currently no predicted genes *F matching this sequence in the Berkeley Drosophila Genome Project Database. *F Its sequence and translation are listed below: *F >cdna fasta *F TACATATTACATACAATTTACATATAAGTACGAAAAAACTTTCCTGGGCCGGAAACAGAT *F TCGAAAACAAAGGAGCAAACACCCACATATTTGAAGAGGATTAATCATGGACATCTCAAA *F GGCACCAAATCCGCGAAAACTGGAGCTGTGTCGCAAATACTTCTTTGCTGGCTTTGCATT *F TCTGCCCTTTGTGTGGGCCATTAACGTTTGCTGGTTTTTCACGGAGGCCTTCCATAAGCC *F ACCATTTTCGGAGCAGAGCCAAATAAAGAGATATGTTATATACTCTGCAGTGGGGACTCT *F ATTCTGGCTGATAGTACTAACTGCCTGGATAATAATATTCCAGACAAATCGCACAGCCTG *F GGGCGCCACAGCGGACTATATGAGCTTCATCATACCCCTAGGCAGTGCATAGACATAACT *F AGATTAATTCGTTAGCAACTAATGATATTAAAAAAGACTTCATTCCTAAACAAAATTAAC *F GTTTATTATTAATCCA *F >Translation fasta *F MDISKAPNPRKLELCRKYFFAGFAFLPFVWAINVCWFFTEAFHKPPFSEQSQIKRYVIYS *F AVGTLFWLIVLTAWIIIFQTNRTAWGATADYMSFIIPLGSA *F Thanks, *F Dan *F ____________________ *F Daniel Curtis, Ph.D. *F Exelixis, Inc. *F 170 Harbor Way *F P.O. Box 511 *F South San Francisco, CA 94083-0511 *F phone: 650-837-8244 *F Fax: 650-837-7620 *F curtis@exelixis.com # *U FBrf0146798 *a Reim *b I. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:51:29 2002 *F To: ingolf.reim@mssm.edu *F Subject: Helping FlyBase: ADRC-10371 *F Dear Ingolf, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Developmental functions of Tbx6-related genes in Drosophila.' *F You mention genes some of which are new to FlyBase. We already have a *F record for Dorsocross (CG5133: FBgn0028789). Which of Dorsocross1, *F Dorsocross2 and Dorsocross3 is this? Do you know which of the Genome *F Project CG annotations your other two genes correspond to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Also, if Dorsocross1, Dorsocross2 and Dorsocross3 have picked up a *F short symbol since you submitted the abstract then this would be a good *F time to tell me so I can get them into the database. I can suggest *F Dsc1, Dsc2 and Dsc3 \- I know that they are not currently taken by any *F other gene \- but feel free to make your own suggestions if you would *F rather. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Ingolf.Reim@mssm.edu Tue Mar 19 23:33:08 2002 *F Subject: Re: Helping FlyBase: ADRC-10371 *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F The Dorsocross gene already entered in Flybase (CG5133) corresponds to *F Dorsocross1 of my ADRC abstract. There are three similar genes located in *F the same region. To make it more clear which one is the subject I would *F suggest renaming Dorsocross to Dorsocross1. Dorsocross2 is the gene next *F to it, CG5187 ,and Dorsocross3 is CG5093. As short symbol I suggest *F keeping the already existing Doc plus adding the corresponding numbers *F leading to Doc1, Doc2 and Doc3. This avoids name confusion with the non- *F related Doc element. *F Sincerely, *F Ingolf # *U FBrf0146799 *a Cheney *b C. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:35:17 2002 *F To: ccheney@pomona.edu *F Subject: Helping FlyBase: ADRC-10741 *F Dear Clarissa, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Deficiencies that dominantly interact with rab GDP dissociation *F inhibitor (GDI).' *F You mention a gene that is new to FlyBase, fs(2)AL289. Do you have a *F map location for your gene? This is a data class we like to track, if *F possible. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From CMC04747@pomona.edu Wed Mar 20 00:36:25 2002 *F To: 'Rachel Drysdale ' *F Subject: RE: Helping FlyBase: ADRC-10741 *F Rachel-- we think it's in 49F. Don't have a recombinational map position, *F though. *F Cris # *U FBrf0146800 *a Kanuka *b H. *t 2002.3.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:24:35 2002 *F To: kanuka@brain.riken.go.jp *F Subject: Helping FlyBase: ADRC-10457 *F Dear Hirotaka, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila ENDD2, a regulator for neural cell death and degeneration *F identified by gain-of-function screen.' *F You mention a gene that is new to FlyBase, endd2. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kanuka@brain.riken.go.jp Wed Mar 20 05:53:42 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10457 *F Dear Dr. Rachel Drysdale, *F I 'd like to thank all of you and Flybase colleagues for your continuous *F efforts and kindly supports in Drosophila research fields. The endd2 gene is *F identical with CG9539/Sec61alpha. The accession number for the endd2 sequence *F is AB062670 (CG9539/DSec61a). *F Thank you very much. *F Best regards, *F Hirotaka *F \------------------------------------------ *F Hirotaka Kanuka, Ph.D. *F Lab. Cell Recovery Mechanisms *F Brain Science Institute (BSI), RIKEN *F 2-1 Hirosawa, Wako, Saitama 351-0198, Japan *F TEL: \+81-48-467-6945 *F FAX: \+81-48-467-6946 *F E-mail: kanuka@brain.riken.go.jp # *U FBrf0146801 *a Hay *b R. *t 2002.3.20 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:51:45 2002 *F To: rhays@pcg.wustl.edu *F Subject: Helping FlyBase: ADRC-10379 *F Dear Rebecca, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The E2 ubiquitin conjugase Morgue mediates apoptosis in the developing *F retina by promoting degradation of Diap-1.' *F You mention a gene that is new to FlyBase, morgue. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From rhays@pcg.wustl.edu Wed Mar 20 13:59:12 2002 *F Subject: Re: Helping FlyBase: ADRC-10379 *F To: 'Rachel Drysdale (Genetics)' *F Hi, Rachel. The morgue transcript is CT35501, and the cDNA is *F BcDNA:GH02435. The accession number is AF145603. I've never seen a CG *F number for it. *F \------------------------------------------------- *F Rebecca Hays *F Molecular Biology and Pharmacology *F Washington University School of Medicine *F Campus Box 8103; 660 S. Euclid Avenue *F St. Louis, Missouri 63110 *F phone: 314-362-7797 fax: 314-362-7058 # *U FBrf0146802 *a Singh *b N. *t 2002.3.20 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:52:28 2002 *F To: singh@wadsworth.org *F Subject: Helping FlyBase: ADRC-10384 *F Dear Navjot, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Drosophila Sin3A-associated polypeptide p18 (dSAP18), a co-repressor that *F interacts with Bicoid and inhibits its activity.' *F You say 'To further study dSAP18 function, we generated dSAP18 mutants *F by remobilizing a P-element located 259bp away from dSAP18 start *F codon.' Is this a BDGP or other public P-element? If so would you be *F able to tell me which one it is? We have records for them all in *F FlyBase and we like to keep all the various data points correctly *F inter-related as much as possible. *F You mention a mutation called Delta-dSAP18-117 which deletes 2/3 of the *F coding sequence of Bin1 (dSAP18). Depending on whether this deletion *F also removes any part of any of the transcription unit I will make *F either an allele record (Bin1117) or an allele record (Bin1117) *F with a linked deletion chromosome record (Df(3R)Bin1117). Could you *F confirm for me that the deletion extends from the progenitor insertion *F to 2/3 of the way into the coding region of Bin1 only? *F Sorry to seem so particular \- but these questions do a make a *F difference to how we put the data into FlyBase and we like to get *F things as correct as possible at the point at which they go into *F FlyBase. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From singh@wadsworth.org Wed Mar 20 19:27:21 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10384 *F Dear Rachel, *F In dSAP18 deletion screen, I used the P-element insertion line EP(3)3462 *F from BDGP database. This P-element is located 259 bp from start codon of *F dSAP18. *F The dSAP18 deletion I used in my abstract Bin1(117), is 601 bp deletion *F from p-element insertion site. So to be exact it removes 448bp out of 633bp *F dSAP18 gene. I hope it answers your questions. If not please do not *F hesitate to ask again. *F Thanks *F Navjot # *U FBrf0146803 *a Gorski *b M. *c J.E.J. *d Eeken *t 2002.3.21 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:31:06 2002 *F To: m.gorski@lumc.nl *F Subject: Helping FlyBase: ADRC-10572 *F Dear Marcin, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Drosophila rad50 mutants are pupal lethal, however the third *F instar larvae show elevated levels of anaphase bridges in dividing *F cells.' *F You mention a gene that is new to FlyBase, rad50. Do you know which of *F the Genome Project CG annotations your gene corresponds to? Is it by *F any chance CG6339? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F You also mention a P{EP} insertion mutant. Is this one of the public *F collection of EP lines? If so I would be most grateful if you could *F tell me the insertion identifier. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From M.Gorski@lumc.nl Thu Mar 21 10:23:07 2002 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10572 *F Dear Rachel, *F It appears that gene CG6339 encodes a protein that shows high homology *F to yeast and human Rad50 proteins involved in DNA double strand break *F repair and the following information was obtained from the FlyBase. The *F map positions of CG6339 as well as the EP(2)2600 insertion were also *F from the FlyBase. In addition to the information provided by the FlyBase, *F we confirmed the exon-intron structure of the CG6339 gene by sequencing *F the SD05424 EST clone as well as the 5' 1.5 Kb cDNA fragment obtained *F from the RT-PCR reaction. *F Furthermore, we utilized EP2600 insertion inserted roughly 750bp upstream *F of the CG6339 to perform P-mediated mutagenesis. We identified one insertion *F into the third exon of the CG6339. The phenotype of Dmrad50EP1 mutant flies *F is described in the abstract the flies are still in our collection. *F Sincerely, *F Marcin # *U FBrf0146804 *a Bloomington Drosophila Stock Center *b ?. *t 1995.5.17 *T personal communication to FlyBase *u *F >From matthewk@fly.bio.indiana.edu Wed May 17 10:20:23 1995 *F To: flybase@morgan.harvard.edu *F Subject: stock list loading results \- One *F > \-- Re1 / Rough eye, EMS-induced dominant rough eye *F > mutation on 3, unmapped, complements Df(3L)R for viability (K. *F > Matthews), synonym W23, Barbara Wakimoto # *U FBrf0146805 *a Gupta *b T. *t 2002.3.21 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:46:24 2002 *F To: tgupta@princeton.edu *F Subject: Helping FlyBase: ADRC-10209 *F Dear Tripti, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of cct1 function during oogenesis.' *F You mention two genes that are new to FlyBase, cct1 and cct2. Do you know *F which of the Genome Project CG annotations your genes correspond to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your genes do not correspond to CGs then *F perhaps you could tell me their map location, as this is valuable *F information for the genome annotation project. *F I have looked into this and wonder whether cct1 and cct2 correspond to *F CG1049 and CG18330? Also, you say 'we have generated mutations in cct1 *F by excising a P-element inserted in the 5'UTR of the gene'. Would this *F P element be P{EP}3346 or P{EP}3379 in CG1049 by any chance? We *F already have a record in FlyBase for Cct (FBgn0041342) based on an *F earlier abstract from you \- I imagine that this record is a forerunner *F to the individual records that I will make for cct1 and cct2, i.e. that *F the gene you originally described as Cct is now known to be cct1 and *F cct2. If you could confirm this for me (or otherwise set me straight) *F I would be most grateful. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From tgupta@phoenix.princeton.edu Thu Mar 21 17:45:06 2002 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10209 *F Dear Rachel, *F Your analysis of the cct genes is correct--cct1 corresponds to CG1049 *F and cct2 corresponds to CG18330. The gene that I previously called 'cct', *F I am now calling 'cct1'. At the time, I didn't realize that there was a *F second homolog. We have made P-element excisions, but used an insertion *F line that was generated in our lab (called BN81). We have not used any of *F the EP lines for this purpose. *F Best regards, *F Tripti # *U FBrf0146806 *a Quinn *b L. *t 2002.3.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:24:54 2002 *F To: l.quinn@pmci.unimelb.edu.au *F Subject: Helping FlyBase: ADRC-10471 *F Dear Leonie, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The Drosophila homolog of the c-myc transcriptional repressor FIR inhibits *F growth and proliferation.' *F You mention a gene that is new to FlyBase, Fir (dFIR). Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From l.quinn@pmci.unimelb.edu.au Fri Mar 22 00:25:00 2002 *F To: Rachel Drysdale *F Subject: RE: Helping FlyBase: ADRC-10471 *F The gene we called dFir was is entered in Flybase as pUbsf with CG number *F 12085. However in a recent publication (Buskirk and Shupbach, Dev. Cell, 2 *F p343-353)the gene has been called half pint (Hfp). *F If you need more information let me know. *F Leonie *F Leonie Quinn, PhD *F Dept. Cell Cycle & Development *F Peter MacCallum Cancer Institute *F St Andrew's Place *F East Melbourne *F Vic, 3002 *F Australia *F Ph: 0061 3 9656 1282 *F Fax: 0061 3 9656 1411 # *U FBrf0146807 *a Bauer *b R. *t 2002.3.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:48:23 2002 *F To: r.bauer@uni-bonn.de *F Subject: Helping FlyBase: ADRC-10291 *F Dear Reinhard, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification and analysis of the Drosophila longevity protein.' *F You mention a gene that is new to FlyBase, Lag1. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From r.bauer@uni-bonn.de Fri Mar 22 08:49:55 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10291 *F Dear Rachel, *F I am sorry for the late response and I want to apologize. Here the *F answer to your question: *F The gene I am referring to corresponds to CG15898 and CG3576. *F I hope this will help. *F Greetings *F Reinhard # *U FBrf0146808 *a Nash *b H. *t 2002.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:45:19 2002 *F To: nash@codon.nih.gov *F Subject: Helping FlyBase: ADRC-10161 *F Dear Howard, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Synaptic plasticity and locomotor behavior are altered by mutations in a *F novel ion channel.' *F You mention a gene that is new to FlyBase, alpha-1U. Do you know which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F >From nash@codon.nih.gov Sat Mar 23 21:11:38 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10161 *F Hello Rachel: *F The gene referred to in my abstract is listed in Gadfly as CG1517. The *F name Dm-alpha1U comes from the review by Littleton and Ganetzky that came *F out in Neuron at the time the fly genome was published. The U in the name *F indicates that the family formed by orthologs of this gene in C. elegans, *F D.melanogaster, and R. norvegicus is Unique in that it is clearly *F evolutionarily distinct from all the well known families of four-repeat *F six-TM ion channels (sodium channels, L-type calcium channels, etc). The *F annotation for the gene in Gadfly is reasonable but we do believe that the *F ORF starts substantially downstream of the place they indicate. *F In Flybase, the mutants we have identified in CG1517 are listed under *F the gene called 'narrow abdomen'. The most serious problem with this *F entry is the fact that all existing stocks of the putative P element *F allele, P890, do not have a P element in this gene (or anywhere else on *F the X chromosome). What this means for the 'excision lines' obtained *F from this element, I do not know. Since our discovery of this problem *F (well before the publication of the 1997 Genetics paper describing this *F allele), I have asked Krishnan to fix this error. But, although he has *F confirmed our result, he has not done so. *F Hope this is all the information you need. *F Howard # *U FBrf0146809 *a Culi *b J. *t 2002.3.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:55:10 2002 *F To: jc1063@columbia.edu *F Subject: Helping FlyBase: ADRC-10447 *F Dear Joaquim, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of a new gene involved in the development of the proboscis.' *F You mention a gene that is new to FlyBase, boca. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jc1063@columbia.edu Fri Mar 22 21:53:30 2002 *F To: *F Subject: Re: Helping FlyBase: ADRC-10447 *F Dear Rachel Drysdale, *F I am writing to you regarding your request for information about the *F newly characterized gene boca, that we will present in the next ADRC *F in San Diego. *F boca corresponds to CG1364 but please, note the FlyBase error report *F for CG1364 from Bernadette Holdener (FBrf0131065) that suggested *F CG1364 is in fact a merge of two independent genes. We agree with her *F interpretation. boca corresponds to the 5' moiety of CG1364. *F Moreover, we sequenced CG1364 transcription unit in the stock *F l(2)43Ea and found a point mutation inside the ORF, suggesting that *F CG1364 and l(2)43Ea are in fact the same gene. *F I hope this information will help avoid duplications during the *F annotation of Drosophila Genome. Please, do not hesitate to contact *F me if you need additional information. *F Best wishes, *F Dr. Joaquim Culi *F Joaquim Culi *F Dept. of Biochemistry and Molecular Biophysics. *F Columbia University *F 701 West 168th Street, HHSC 0602 *F New York, NY 10032 *F Phone: 212-305.2111 *F e-mail: jc1063@columbia.edu # *U FBrf0146810 *a Yoshida *b S. *t 2002.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:31:38 2002 *F To: yoshida@embl-heidelberg.de *F Subject: Helping FlyBase: ADRC-10623 *F Dear Shoko, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A mutation involved in restriction of oskar activity to the posterior pole *F during Drosophila oogenesis.' *F You mention a gene that is new to FlyBase, l(3)18304. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Also \- is this a BDGP insertion? If so we ought to have a record of it *F but we cannot find anything with the identifier. Finally, it would be *F useful if you could tell me which element is inserted in *F l(3)1830418304 mutants \- P{PZ}, P{lacW} or whatever. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From yoshida@embl-heidelberg.de Sat Mar 23 17:46:42 2002 *F Subject: Re: Helping FlyBase: ADRC-10623 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F Thank you very much for your message, I'm sorry to be late in *F replying. *F > You mention a gene that is new to FlyBase, l(3)18304. Do you know *F > which of the Genome Project CG annotations your gene corresponds to? *F In fact, we still on the way of characterizing the gene for l(3)18304. *F The approximate map location is *F around 77F1-2, which was determined by the complementation with some *F deficiencies in that region. *F > Also \- is this a BDGP insertion? If so we ought to have a record of it *F > but we cannot find anything with the identifier. Finally, it would be *F > useful if you could tell me which element is inserted in *F > l(3)1830418304 mutants \- P{PZ}, P{lacW} or whatever. *F In fact this is not a BDGP insertion but is generated *F in our lab some years ago. *F The situation is a bit complicated, since we got this mutation *F as P(P{lacW}) insertion, but we found that *F P is not associated with the phenotype(zygotic semi-lethal, *F maternal effect lethal), *F so we now think the mutation we're interested in is spontaneous. *F The way we name this gene could cause confusion, since it *F indeed sounds like a P-induced mutant, and we believed it as such *F in the beginning, but we still haven't re-named them.. *F If there is anything unclear, or more detailed information is needed, *F please contact me again. *F All the best, *F Shoko # *U FBrf0146811 *a Williams *b B. *t 2002.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:28:51 2002 *F To: bw28@cornell.edu *F Subject: Helping FlyBase: ADRC-10522 *F Dear Byron, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of the Zw10/Rod complex by immunoaffinity chromatography.' *F You mention a gene that is new to FlyBase, zwilch. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From bw28@cornell.edu Sat Mar 23 21:37:04 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10522 *F >Dear Rachel, *F Sorry \-- *F 'Zwilch' gene is CG18729 *F Thanks, *F Byron # *U FBrf0146812 *a Borge *b K. *t 2002.3.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:23:44 2002 *F To: karin.borge@ucmp.umu.se *F Subject: Helping FlyBase: ADRC-10025 *F Dear Karin, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The PGRP-LC gene cluster, a complex region involved in the activation of the *F immune response.' *F You mention a gene that is new to FlyBase, PGRP-LF. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Karin.Borge@ucmp.umu.se Mon Mar 25 14:28:26 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10025 *F Hi, *F I'm sorry that I haven't replied sooner, but I've been busy preparing stuff *F for the drosophila meeting. *F PGRP-LF corresponds to CG4437. *F Best wishes *F /Karin *F Karin Borge, Umea Centre for Molecular Pathogenesis (UCMP) *F UCMP building 6L *F Umea University *F 90187 Umea *F Sweden *F e-mail: Karin.Borge@ucmp.umu.se *F phone: 46-(0)90-7856788 *F fax:46-(0)90-778007 # *U FBrf0146813 *a Bonaccorsi *b S. *t 2002.3.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:43:42 2002 *F To: silvia.bonaccorsi@uniroma1.it *F Subject: Helping FlyBase: ADRC-10994 *F Dear Elisabetta, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Spindle assembly and cytokinesis in the absence of chromosomes during *F Drosophila male meiosis.' *F You mention genes that are new to FlyBase, fsl and suo. Do you know *F which of the Genome Project CG annotations your genes correspond to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your genes do not correspond to CGs then *F perhaps you could tell me their map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Silvia.Bonaccorsi@uniroma1.it Mon Mar 25 10:12:50 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10994 *F Dear Rachel, *F here are the informations about fsl and suo that you requested. *F Unfortunately, we do not know yet which of the Genome Project CG *F annotations these genes correspond to. *F We can just provide you with their map positions, that are 71C-D for fsl *F and 37D-E for suo. *F Best regards *F Silvia Bonaccorsi # *U FBrf0146814 *a Osterwalder *b T. *t 2002.3.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:37:50 2002 *F To: thomas.osterwalder@yale.edu *F Subject: Helping FlyBase: ADRC-10845 *F Dear Thomas, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Neuroserpin may Regulate Proteolytic Processing of Neuropeptides.' *F You mention a gene that is new to FlyBase, neuroserpin. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Also, if neuroserpin has picked up a short symbol since you submitted *F the abstract then this would be a good time to tell me so I can get it *F into the database. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From thomas.osterwalder@yale.edu Mon Mar 25 17:56:32 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10845 *F Hi Rachel, *F sorry to be late with this reply. The Drosophila Neuroserpin like cDNA *F corresponds to sp4, CG9453, a serine proteinase inhibitor first described *F by Han et al (2000). One of the splice variants of this Serpin shows *F considerable homology to the vertebrate Neurosepin proteins (Human, mouse, *F rat, chicken). In the work I intend to present at the fly meeting, I used *F both, the Drosophila sp4 cDNA and a vertebrate (chicken) neuroserpin cDNA *F under the control of the GAL4 UAS to investigate mis/overexpression *F phenotypes. Since I found very similar phenotypes for overexpression of *F chicken Neuroserpin and Drosophila sp4, and because of the sequence *F similarity, I refer to the proteins in the title of my abstract as *F 'Neuroserpin'. Other features of vertebrate neuroserpin (like the *F neuron-specific expression) may be less conserved in flies, and I haven't *F found a bona-fide mutant yet (with a phenotype that would justify to name *F the gene), so I am reluctant to (re)name the gene and would probably stick *F with sp4 for right now. *F I hope this helped a little. *F Best, *F Thomas # *U FBrf0146815 *a Zahedi *b B. *t 2002.3.25 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:53:12 2002 *F To: bzahedi@sfu.ca *F Subject: Helping FlyBase: ADRC-10396 *F Dear Bari, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of putative effector proteins for the small GTPase Dcdc42 in *F Drosophila.' *F You mention a gene that is new to FlyBase, Cip4. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From bzahedi@rm-rstar.sfu.ca Mon Mar 25 23:37:35 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Re: Helping FlyBase: ADRC-10396 *F I can tell you some stuff now and send you any sequence that I have. *F None of this is yet published and I still need to prove that it is an *F effector of Dcdc42 so please take that into consideration ( ie it is *F just a gene with homology to the mammalian CIP4 for now). The CG *F number is 15015. It originally was split into two predicted genes ( *F the other being CG11341)but now has been fixed ( info is on old version *F of scaffold AE003481 if you are interested)I have sequenced LD14951 and *F its translated sequence lines up with the mammalian CIP4 quite well. *F Please let me know if you would like any sequence data I have or if I *F can be of further assistance, *F sincerely, Bari Zahedi # *U FBrf0146816 *a Eacker *b S. *t 2002.3.26 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:46:42 2002 *F To: seacker@genetics.washington.edu *F Subject: Helping FlyBase: ADRC-10221 *F Dear Stephen, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Paternal chromosome loss caused by the paternal loss and loser mutations of *F Drosophila melanogaster.' *F You mention a gene that is new to FlyBase, lsr. Do you happen to know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From seacker@gs.washington.edu Tue Mar 26 02:01:09 2002 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10221 *F Rachel- *F We only have a rough map position for loser right now. I'm not *F sure that its good enough to be 'curated'. We're certain that its on *F 3R between ru and h, but that's as far as we're at right now. Thanks! *F Steve *F \-- *F Stephen Eacker *F Department of Genome Sciences *F University of Washington *F Lab: (206) 543-6719 *F 'Time's fun when you're having flies.' # *U FBrf0146817 *a Iida *b T. *t 2002.3.26 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:36:38 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 18 Mar 2002 09:36:38 \+0000 *F To: takakoi@box-t.nih.gov *F Subject: Helping FlyBase: ADRC-10814 *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 18 Mar 2002 09:36:36 \+0000 *F Content-Length: 1247 *F Dear Takako, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'missing oocyte (mio), a gene required to maintain the meiotic cycle in the *F oocyte.' *F You mention a gene that is new to FlyBase, mio. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From takakoi@box-t.nih.gov Tue Mar 26 03:23:08 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 26 Mar 2002 03:23:08 \+0000 *F Mime-Version: 1.0 *F X-Sender: NIH\takakoi@NIHEXCHANGE4.nih.gov *F X-Mailer: Macintosh Eudora Pro Version 4.2.1-J *F Date: Mon, 25 Mar 2002 22:23:14 \-0500 *F To: Rachel Drysdale (Genetics) *F From: Takako Iida *F Subject: Re: Helping FlyBase: ADRC-10814 *F Content-Transfer-Encoding: 7bit *F Dear Rachel, *F Thank you to remind me to reply to your letter. The results from 5' and 3' *F RACE of mio mRNA show that mio gene corresponds to two CGs, CG7074 and *F CG15384. On the Flybase, the predicted 3'end of CG7074 mRNA cut actual mio *F mDNA at the 6th exon. CG15384 is 7th exon of mio gene. If you need more *F information, please feel free to ask me. Thank you. *F Takako # *U FBrf0146818 *a Nichols *b R. *t 2002.3.23 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:23:17 2002 *F To: nicholsr@umich.edu *F Subject: Helping FlyBase: ADRC-10019 *F Dear Ruthann, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Flatline, a novel peptide, stops Drosophila melanogaster heart rate.' *F The flt gene is new to FlyBase and though we tested the sequence by *F BLAST the best hit was to Ast2 (FBgn0032336) and you say explicitly *F that flt does not encode an allostatin. Since the genome has been *F sequenced it is possible that a CG gene annotation corresponds to flt. *F Do you know which of the Genome Project CG annotations flt corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If your genes do not correspond to CGs *F then perhaps you could tell me their map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From nicholsr@umich.edu Sat Mar 23 23:06:08 2002 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10019 *F Rachel, *F Flt or flatline is not an allatostatin in activity, ie, it does not *F affect juvenile hormone synthesis. Structurally, FLT is the Drosophila *F melanogaster AST C-type peptide, which is also called Drm-AST C and *F probably other names, too, unfortunately...sorry. *F If this helps, great, otherwise, please get back to me. *F regards, \- Ruthann # *U FBrf0146819 *a Abrams *b E. *t 2002.3.26 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:31:22 2002 *F To: eabrams@jhmi.edu *F Subject: Helping FlyBase: ADRC-10605 *F Dear Elliott, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Regulation and function of PH4alphaSG1 and PH4alphaSG2 in the Drosophila *F embryonic salivary gland.' *F You mention genes that are new to FlyBase, PHaSG1 and PHaSG2. Do you *F know which of the Genome Project CG annotations your genes correspond *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. If your genes do not correspond to CGs *F then perhaps you could tell me their map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From eabrams@jhmi.edu Tue Mar 26 17:37:32 2002 *F Subject: Re: Helping FlyBase: ADRC-10605 *F To: Rachel Drysdale (Genetics) *F Hi Rachel, *F I apologize for not getting back to you sooner (our *F e-mail server was recently changed and so access was *F restricted). Most of the info you requested has been *F recently published in Mechanisms of Development (112 *F 165-171 (2002). Sequences are also available in *F genbank. I will give you the info concerning the *F CG's here (+cDNA sequence). During the course of our *F study we learned that a few parts of their sequence *F (the CG's) were inaccurate (due to gene structure). *F If there are any questions feel free to contact me. *F PH4aSG1 (Kauvar 1.7)- CG15542 *F agttttaaatgggacactgaacagtcaaaATGTTCCGGCTAAGTATTATT *F TTACCATTACTCTGCTTATTCCCAGTCAATGGAGATTACTACTCAGCTAT *F AAGTGAGCTGGAAAGACTACTCGACGTGGAGGCTTTTATAGTAGAAAAGT *F TCGATGAATATCTTGAAAGGGCTCAGCAAGAACAGGAGAACTTGAAAAGA *F TTTTTAGATCAGATAGACGAGCAGCAAAATGATCGTGGCGATCTCGAGGA *F ATACTTTGGAAATCCAATCAATGCCTTCATCACCATTAAACGCCTCGTTC *F ATGACTGGAAGTTCAATGTATTTGATCCTGTGTTTGACTCCAGTAATTTT *F GAGACCTACAAGAACAACTTAAGTGATTCGCTCGAGAAGATCAACTTCAA *F AGGACCCACCCAAGAGGATCTACATGGAGCCACCCGCGCTCTTCTTCGGC *F TGCAGAATATGTACCAACTAAACACGGACCACCTAGCTTCTGGCGTTCTC *F CTTCCTGGCGAGAAAAGCCCACTGGCCACCTCGTTGAGTGCAAGTGACTG *F TTTTGAGTTGGGCAAGAATCTCTGCGAGATTAAGGAGTACTCTTACGGTT *F CCGAGTGGCTATTGGAGGCCAGAAAACGATTGCACGGGAAACCATTAGGA *F TTTATATCACCCAATGTGAGTGATGTCGAGATACTGGAGCACCTTTCACC *F CGCCTTCAATGGTTTGGGAAATCTAAAATTAGCTCATAAATTAAATAATG *F AAATATTGGATAAGAAGCCTGATCATGAGGAGGCTCTCAAGAACAAGATA *F TTGTACGAAGGCCAATTGGCTAGGGAGCGAAGTTTTGCTCCCAGAAAGCA *F AGTGGAACTGCCTCATATAGCAGAAAAAGAGCAAAAGGAGAGCTACAAGC *F TATATACGGAAGTTTGTCGAGGAGAACTCCATCAATCTCCGCGGGAGCAG *F CGGAATTTGAGGTGCTGGCTCTCCCACCAGGGAGTTCCCTACTATCGTCT *F CTTCCCTTTTAAAATCGAGCAATTGAATATCGACCCTTATGTCGCCTACG *F TTCACGAAGTTCTTTGGGACAGCGAGATAGATACGATCATGGAGCACGGC *F AAGGGTAATATGGAGCGTTCAAAGGTGGGTCAAAGCGAGAACTCTACCAC *F AAGTGAGGTCCGGATAAGTCGCAACACCTGGCTATGGTACGATGCGAATC *F CATGGCTTTCCAAGATTAAACAGCGTCTGGAGGATGTCACCGGGCTGAGC *F ACGGAAAGTGCGGAGCCCCTGCAGCTGGTCAACTACGGAATTGGGGGACA *F GTATGAGCCGCACTTTGACTTCGTGGAGGACGATGGTCAAAGTGTTTTCA *F GCTGGAAGGGCAACCGCCTGTTGACAGCTCTTTTCTACCTAAATGATGTT *F GCTCTGGGAGGAGCCACAGCCTTTCCCTTCCTCCGATTGGCTGTTCCGCC *F GGTGAAGGGAAGTCTTTTAATCTGGTACAATCTGCACAGTTCCACCCACA *F AGGACTTTCGCACGAAGCATGCTGGTTGCCCCGTTCTTCAAGGATCAAAG *F TGGATTTGCAATGAGTGGTTCCATGTGGGTGCCCAGGAATTCaggcgacc *F ttgtggcttgagcagtgatgagggaaagtttttgcacttgaaggacatat *F aa *F PH4aSG2- CG1546 *F CTCGTGGTTCACAGTGCTCTGGTCATAATGTTGGATCGGCACTGTCTTTA *F TATTGGGATTTTCCAGCTTATAATTTGGGTTGGAGTAGCGAACGGTGAGT *F TTTACTCATCGGTGGACAGTATGCAGGATCTAGCACAAGTGGAAGAAGAA *F CTCCTAAATGCCACGCGTTCATATGTGGAGTCCCAGCAAAAGCAGCTTGA *F CTTCTACCGCAGGTATGTTGAGCAAATAAAACGGGAACATGAGTGGGCGA *F CATCCCAATTAAAGCTGGATGACTATCTCGGGCACCCATTGCACGCCTTC *F AGATTGATCAAGCGATTGGTTCGGGATTGGGATTCGCTGATCTTTGAACC *F CATACTCGCCAACAACGCCAGAGAAGAGTTCCGTGCATTCGTGGAGGTTC *F TGAGCAGGGACTTGGGCTACCCAGATCAATCTGAGCTCCAGGGAGCCATC *F AAGGGCTTGGCCAGGCTACAAAAGGTCTATAATCTCGCGACCTCCGATTT *F GGCCGATGGCATCATCGGCGGTTTAAACTACGGCTCGGACTTACGATGGC *F GTGAATGCTACGAAATCGGTGTACAGCTCTTCGATCTCGGAGAATATCAG *F AGATCCCTGGAATGGCTTCAAGTTGCATTTATCCTTCTTCGAAATAGTCC *F AAGAGAGGAAAAAGATGCGGATCACTACCTTTCAGATATCCGTGAGTATG *F CATCCATGGCTAATTTTGAGTTGGGCAATCCAAAGAAAGCCGCAAGACTA *F CTGAGTCAGATCCTTGAATCGCAACCAACACATTCCGCCCAGCAAACTCA *F AAAGTATCTGGAGAGCAGGGTGCCAGGAAAGAATGTCCAGGAGACGAAAC *F CCTCTTGGTTTTCCAACTATACTAGGTTATGCCAGGGCAGAAGATTGCCC *F GAAGAGCGAAGTGGTGATCCCTTAAGATGCTACCTGGATGGAAAACGCCA *F TGCTTACTTCACCTTGGCTCCACTTCAAGTGGAACCGGTACACTTGGATC *F CGGATATAAATGTTTATCACGGAATGCTGAGCTCCAAACAGATTTTGTCC *F ATCTTCGAGGAAGCGGACAAGGAGGAGATGGTTAGGTCCGCTGTGGCCGG *F AAGTGGTGGAGAAGGCACCGTGAGGGACTTGCGGGTCAGTCAGCAAACCT *F GGCTCGACTACAAGTCCCCGGTGATGAATTCCGTAGGTCGGATCATCCAG *F TTTGTATCTGGATTCGATATGGCCGGAGCGGAGCATATGCAGGTGGCCAA *F CTACGGAGTTGGAGGGCAGTACGAGCCGCATCCGGATTACTTTGAGGTCA *F ATCTGCCGAAGAACTTCGAAGGAGATCGCATCTCCACCAGCATGTTTTAT *F CTTTCTGATGTGGAACAAGGAGGTTATACTGTCTTCACCAAGCTTAATGT *F GTTCCTGCCTCCTGTCAAAGGAGCTCTGGTCATGTGGCACAATCTTCATC *F GATCCCTGCATGTGGATGCTCGCACTTTGCATGCTGGCTGTCCAGTTATC *F GTGGGTTCCAAGCGGATTGGCAACATCTGGATGCACTCGGGATACCAGGA *F GTTCCGTCGTCCCTGCAACCTCACCTCAGATAGCTACAAGTCGCTAGCCT *F ATCGAGATTAGGTGTAATTTAATATAATAAACATTTAATAAATTAAAAAA *F AAAAAAAAAAAAACTCGAG *F PH4aEFB \-CG12088 and CG1473 *F ATTTCTCGGCGCGCGCACGCGAATTAAACAGAAACACAAATTATATTTTT *F GTAGTCAAAACTGTGAAAAAGGGAATTTACTCCTCGGGGGACCAGAGAAA *F ACAATCCTTAAATTCGAGTCTAAACGAAAATATCTAAAACAAACGTGATT *F TGTGTGCTTCCAGTTAGAGTGCATAAAATGTATCTATAAGATACGAAGTG *F CGGGTGGGCAATGCAATCGAGGTGTTCTCGTTGAATTGTAAACAAAGAAG *F GCGGCCAGTTTCATTGACATCCATTGAGACCCAGCAACAAGTTCGCATAA *F GAAACACAGGAGACTTCAAAATGGCAGCGGACTGGCGATTGATGCTCCTG *F CTGGGAATCCTGCTCCTTGTGGGTGGTCCTGCTAACGGAGAGGTGTACAC *F GGCCTTGGCCGAGATGGAGGAACTTCTGGAGACTGAATCCGTACTGATTA *F CCAACTTGGAGGGCTACATACGCGTCCAGGAGGACAAGCTGAACTTTCTC *F AAGAACAAAATGGACGAGTACCAGAGGGAGCACTCGGATGCCTCCCATGA *F TATAACCGCCTATGTATCCAATCCAATCAATGCGTACCTGCTGACTAAGC *F GGCTAACCACCGATTGGCGGCAGGTGGAGAACCTCATGGAGCACGACGTG *F GGCACGGATTTCTTGCAGAACATCACCCAGTATCGCAGTCTCCTGAAGTT *F CCCCTCCGACGAGGACCTCAATGGCGCGGCGGTGGCCTTGCTCCGCCTGC *F AGGACACCTACCAACTGGACACTTCGAGTGTGGCGAGGGGCAAGCTGAAT *F GGCATTCAGTACAGCACGGAAATGTCCTCGGATGACTGCTTCGAGTTGGG *F TAGACAATCGTACGTGAACCACGACTACTACCACACGGTGCTCTGGATGA *F ACGAGGCGATGGCCCGCATGCTGGAGGAGCCGACCAACCACACCCAGAGC *F TTCACCAAGGCTGACATCCTCGAGTACCTGGCCTTCTCCACCTACAAGGA *F GGGCAACATAGAGAGCGCGCTGACAATGACCAACGAGCTGCTCCAACTGC *F TGCCCCACCACGAGAGAGCCAATGGAAACAAGCGGTTCTACGAGAAGGAG *F ATTGCCCAGCAGCTGCAATTGCGGAAAATGAAGGGCGACGATGGCACCGA *F TGAAATGCCCAAGTCCGATTTGCCCGTGGCCAAGAGTGACCCGGCCATCT *F TTGATATGACAGAACGAAGGGCTTACGAGATGTTGTGCAGAGGCGAACTG *F AAGCCATCGCCATCGGATTTGCGATCCCTGCGATGCCGCTATGTCACCAA *F CAGAGTACCCTTCCTGCGTCTGGGTCCCCTGAAATTGGAGGAGGTTCACG *F CGGATCCGTACATTGTTATCTATCACGATGCCATGTACGACAGCGAGATC *F GATCTGATCAAGCGGATGGCCCGCCCGCGATTCCGCAGGGCCACGGTCCA *F GAACTCCGTGACCGGAGCCTTGGAGACGGCGAACTACAGGATCAGCAAGT *F CCGCCTGGCTAAAGACCCAGGAGGATCGGGTGATTGAGACTGTGGTGCAG *F CGCACTGCAGACATGACTGGCTTGGATATGGACTCCGCCGAGGAGCTGCA *F GGTCGTTAACTACGGCATTGGAGGTCATTACGAGCCGCACTTTGACTTTG *F CCAGGAAAGAAGAGCAGCGCGCCTTTGAGGGTCTCAATCTGGGCAACCGC *F ATTGCCACCGTTCTATTCTATATGAGTGACGTGGAGCAGGGAGGAGCCAC *F TGTCTTCACATCCCTGCACACCGCTCTGTTTCCCAAGAAGGGAACGGCTG *F CTTTTTGGATGAATCTCCATAGAGATGGACAGGGCGACGTGAGGACCCGC *F CATGCCGCCTGTCCCGTACTCACAGGCACCAAGTGGGTGTCCAACAAGTG *F GATCCACGAGCGGGGACAGGAGTTCCGCAGACCCTGCGACTTGGAGGAGG *F ATCACGGCGAGTTCGCCATCTAATCCGGAGTCTAGTTTGCGTATGCAACG *F AATCTTTAGTGCGAAATCTCATCCTTCGTCATCGGCCATCGACTTCCTAT *F TGTAAATAAAGCACTCAGGCCGAAGAAGGTCGCATCTGTTCTACTTATCG *F GTTCATCGTACATGGGAACCAACTTCAACCTAGCTGTATCCTAGACTCGT *F ATGGAATTTATCTACTTTTGTATATTTGCCTAGTTTAAGTAGTACAAAGA *F AAATTGCAATAAAATCGAATTGGAAATCAAAAAAAAAAAAAAAAAACTCG *F AG *F PH4aPV- CG9713 *F AGTTTGCTCCGGCACGTAGACCCACATAGAACTTCGACCAGAAAGGTAAC *F CCACGATGCGCTCTTCGAAGTGGCTGCTTCCGGTGAGGGTGCTGCTCACC *F TACCAGGTGCTGCTTCTACTCCTCCGCCAGGCGAAAGGCGAGGAGAGCCA *F CTGCACCTCGGTGGCAGGAATGGTCAAGCTGCTCGATTTGGAGGCGCAGC *F TGATCGACAACCTAGAGGATTACGCTGTCGAGCTGGAGAAGAAACTGCAG *F ACCGTAAGAAGGAGCATAACAAGTCTGCGTTTGGAAAATGACAAAGCCCG *F CAGTTCCACGGAGGAGTACTTATCCAATCCCCTGAACTCCTTTTCCCTCA *F TTCGTCGCATGAATCGCGACTGGATTATTTGGCAACTCTACATGGACGAT *F CCGGTGGGTATTTCTCAGGTGGAGCGAATCCAGGAGTTGAGGGAACACAT *F GCCCACCCACACGGATGTGGAGGAGGCTGTCACTGCCTTGGATCGCATTC *F AAAGCACCTATGGCCTTAAAGTGCCGGAAATCTCCCATGGATTCCTCAAT *F GGCAAACAGTACAATGTGAGTCTCACTGTATTGGATACCTATGCCATGGG *F ACAAATCTTATTTGACCAGAAAAACTATTTGGCAGCTGCTAGCTGGATCT *F ATCAGTCCGTTGTATTGATGGAGGCCTTTTCGATGGCTGCTCCGCTTGAG *F ATTTCCAAAAATGAAGTGCGGATGGTATATGCTGAAACCCTGTTAAAGCT *F TAACCAGCACGCCGATGCCCTGAAAGTTGTCAACATTGCTCTGACTGATA *F ACCCGCACGATATTAAACTGTTGCTGAAGAAGTCGGAAATCGAAACGATG *F ATAAGGACGGGCACCAATAATGCGCCTCCGGTAAAGGTTCAGGCAACAGG *F CGTGCCAACTGCCTACCAGATCGGCTGTCGAGGTCAGTTCCCGCCGTCCG *F CGGATAGCAAGCTTTACTGTCTGTACAACAGAACCACTTCGCCTTTTCTG *F ATCCTTGCTCCGCTCAAGATGGAACTGGTGGGCCTGGATCCTTACATGGT *F GCTCTACCACGATGTGCTCTCACCCAAGGAAATCAAAGAGCTCCAAGGTA *F TGGCCACTCCTGGTCTAAAAAGGGCCACCGTCTATCAGGCCTCCTCCGGT *F CGGAACGAGGTGGTGAAGACCAGGACCTCCAAAGTGGCCTGGTTCCCCGA *F TGGCTATAACCCGCTCACTGTTCGACTTAATGCCCGCATCTCCGACATGA *F CCGGGTTCAATCTCTATGGCTCCGAGATGCTGCAGCTGATGAACTACGGC *F TTGGGAGGCCACTACGACCAGCACTACGACTTCTTTAACAAGACTAACTC *F CAACATGACTGCCATGAGTGGCGATCGCATTGCGACAGTACTCTTCTACC *F TGACGGATGTAGAACAAGGTGGAGCCACCGTGTTTCCAAACATTAGAAAG *F GCCGTCTTCCCACAACGCGGATCTGTGGTAATGTGGTACAATCTCAAGGA *F CAACGGTCAAATTGATACTCAAACTCTTCATGCCGCTTGCCCCGTTATAG *F TGGGTTCCAAATGGGTGTGCAACAAGTGGATTCGAGAGCGCGAACAGATA *F TTCAGCAGACCTTGCCTCAAAAAGCGAATGTGATTcTCAGCGAAACTGTT *F TTTAGACAAGtGATaCTATACGACCaCtATAAAACTCAATAAAGTCATAT *F TAGAATCGATAATGCTGTCAATTAAACATTCCCAGCGATAAGAACAATTG *F CAAATCAAGTCGCGtCATTCCGAAGAGGAATAGGTGTTTTCCCCAGAGTC *F GTTCATCAAAATCAAGAAATGGAAATTCCTGCTAAATATCGTTTAATGGT *F CGTGAACCAACCAtTAATTGACATTTTGCATTAAAAGCACAAGATAAGAA *F AGCGGGTTTcTATTTTTAAAAACATGAAAATAAATGCACATCGATCTAGA *F AAAAAAAAAAAAAAA *F PH4aNE2-CG9720 *F CAGAATGCTGTTCTGGAGATTGGTGCTCCTGGGAATGCTCTATCTGTCCC *F TGAGTTTTGGCCAGTTGCGAGAGAACAATGCCCAACAGCGCTTTGCAAGA *F TCTGTGGTGAATATGGATGATATGCTGAACTTAGAGGACGATCTTGTCTC *F AAATGTGGAAAAGCTGGCGGAAGCGCTTGCACGAAAAGCCAAAACCATTA *F AATGGGGCGTCTTCAAGATGATGAAAAGGCGACAGGAGTATAAGTCGTCT *F ATGGAAATTTTTGCCAATCCGATTGACACCTTTTCCCTCATTCGTCACAT *F GCAGTCGAACTGGTTGATGTGGCTATTGTACTTGGAAACGCCTGTTGGCC *F AAGAGGAATTGTTTTTTGTGGACTCAAGGATGCCCCTATTGCCAAAGTAC *F TTTGACTTTATAGATGCCGCTGAAGGCATTAGAAAAATGCAGGCCACATA *F CCAGATGTTTTCCTCAGATATAGCCAAAGGCTTACTAGATGGAGTGCAAT *F ACAACTCTTCTCTAAAACCCATCGATTGTCTCGCAATTGGTCTTCATCTG *F ATGAATAACTCGCGTTGGTATGCTGCCGAGCAATGGATAAGTGCTAGCAT *F TGAAGCCTATGATCAGAAAAGTTCTCAGACCGATATGGAGTTATTGAGGG *F GACCAAAACTGGCTGACCTGTGCAGAATACTGGGTCAAGTACAAATGAAG *F CAGAGAAATCATGAGGGTGCTCTTCAAGCCTATCAGGTTGCGCTAAAGCT *F TTCGCCTCATGACCCAGAAATATATGAGGAGTATCGGATCCTGGAAAAAA *F GAGATCTTACGTTGTCAGATATCGAACCGATTGAACAGGATAAGGACAAC *F TCCCACGAAAGATTGGTTCTTCCGCCCTGTTGCAGTGGTCGCTGTCAAGT *F TCCCCGAAATCTGAGTAATCTATATTGTGTATATAACCATGTTACCTCCC *F CATTTCTGCAACTGGCCCCCATCAAAACGGAGATTCTATCGATTGATCCC *F TTCGTAGTGCTCCTCCATGACATGATCTCCCAAAAGGAGAGTACTCTCAT *F TAGAACTTCGAGCAAGGAACACATGTTGCCGTCTGCCACAACTGACCCAG *F ATGCATCCGATGACGAAACACAAGTCGACACATACCGCACCTCAAAGTCT *F GTTTGGTATAGCAGTGACTTCAACGATACGACCAAGAAGATAACCGAGCG *F CTTGGGAGATGCGACTGGGCTGGACATGAACTCTACGGAGTTCTATCAGG *F TCATTAACTACGGGCTAGGGGGTTTCTTCGAGACGCATTTGGACATGTTG *F CTGTCGGAAAAAAACAGGTTCAATGGAACCAGTGATCGTATTGCCACCAC *F ACTCTTCTATCTTAATGAAGTTCGCCAGGGCGGCGGCACGTACTTCCCTC *F GACTAAATCTCACGGTATTCCCACAGCCAGGATCGGCCCTCTTTTGGTAT *F AACTTGGACACGAAGGGTAACGATCATATGGGTTCCCTGCACACCGGCTG *F CCCGGTCATCGTGGGTTCCAAATGGGTTATGAGCAAGTGGATAAATgATA *F TGGGaCAGGAGTTTaCAAGGCCCTGTGTCGAGTCAAGTTTAAGCTCAAAA *F gAGGTGTTATCAGCTGAGCGGCTTATAATTTGATTTATTTcTTCATTTTT *F ATcCCATAATATAATAATAaGTAGGTAAAAAaAAGGAaGTATATACTATT *F GATGTGCATTATTCGTGGTAAAGTATAaCCAATGGATCAATAAAGAGCaC *F TTCTGCTTTGATTGGTGCGAGCAAAAAAAAAAAAAAAAAAAA *F PH4aNE3-CG9708 *F ATGGCTTATGTTTTGCAAAGTTGAAAGCACATTGAATTGGGAGAGCCGTC *F AGGAATTTCTAGATACGGAATCAGCGAAAATGGACTTAATGCAATTGGAC *F GTCGAATTAATTGATAATCTAATGAATTACGCCGAAAAAATAGATGAGAA *F GGCTTCCCAACTAAAGAGACTAGCCCAGGAACTAAGACAGCCTTTGCACT *F CTGCGAAAGGTAGAGAAGAGGAGTACTTGGGCAATCCTCTCCACAGTTTT *F CCACTCATTCGACACATGTACCAGGATTGGCGGTACCTGGAAGAGTTTAT *F GAAGAAACCAGTGGGAGAAGAGGAAATCCAATTTCTAAGAAGAAAGTTAC *F CGGAACTGCCCTGGCAGGTGGACACCGAGGAAGCCAGCGTCTCAATCTTC *F AGAATAGCTGAAACCTATGGAATGATGCCCTGGGATATGGCCAACGGTCT *F CATCGACAATGTTCGGTTCAACTCCACACTGCCAGCTCTGGATTGTTTTG *F AAGTCGCAAAGATGTACTTCAAGTGGGGCTACTTCAAGCAGGCATTACAG *F TGGATAACCATCAGCAAAGCTCGGATGAAAGAGGAGTACTCCGGGGTTTA *F TGAGGTGCTGGGAATGAATCGCCAGGATGTGGCCCTGCTGCAGGCTCGCT *F GTTTAGTGGAATTGGACCGGAGAGATGAAGCCCATGAGGTACTTCTGGAT *F CAACCTGATCTCGCAGACAATTCGATTAGCTTGCTGGACCAATTCAAGGC *F CAATCCTTACGAGGCAATAGACAGTTCGCCCAAGTTGGGTGAAGGCTACA *F AGAGACTGTGCCGTTCCTCGTTCTCTCCGAACCCTTCAAAACTCCACTGT *F CGCTACAACAGCACCACCTCCGCCTTCCTGATCCTGGCTCCCCTCAAAAT *F GGAGGAGATCTCTCTGGAGCCCCATATAGTGGTGTACCATGACATCCTGC *F CGGATAAGGATATCCAGCAGCTAATAACCTTGGCGGAGCCACTACTGAAA *F CCCACAGAAATGTTTGACGATAATAAGAATGAGGCAAGGAGCAGTTACCG *F CACACCGCTCGGTGGACCACTTTTGGATAGCTTGACTCAGAGGATGCGGG *F ATATCACTGGCCTACAAATCCGCCAGGGAAATCCCATTAATATTATCAAA *F TACGGCTTTGGAGCTCCCTACACTAATTACTATGACTTCTTCAAAAAGAG *F AAACTCGGAATCTAAAGGATTTGGTGATCGGATGGCCACCTTTATGTTTT *F ATCTAAATGATGCTCCTTACGGAGGTGCCACTGTCTTCCCGCGTTTAAAT *F GTTAAAGTACCTGCCGAACGGGGAAAAGTTCTGTTCTGGTACAATCTTAA *F TGGCGACACCCACGACATGGAGCCCACCACTATGCACGCCGCTTGTCCCG *F TCTTTCATGGTTCCAAATGGGTAATGACAGCCTGGATACACGAATATGAC *F TAGATTTTCATTCAACCCATATACCGCGAGGGAAAGACAATGAAACACAA *F ATAATATTACAGCTAAAACTTAAGATAAAACAACAGACGTTTTATTTCAT *F TGGGAAGTAACTAACTAAGCATAACTAACTAACCAATAAACATATCGAAT *F TGAATGTGCAGCGCATAATACCTTTTTAAAAAAAAAAAAAAAAAA # *U FBrf0146820 *a Hanyu *b K. *t 2002.3.27 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:27:17 2002 *F To: hanyu@sakura.cc.tsukuba.ac.jp *F Subject: Helping FlyBase: ADRC-10498 *F Dear Kazuko, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'N14 is a novel maternal effect mutation affecting pole cell maintenance.' *F You mention a gene that is new to FlyBase, N14. Do you know yet which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Also, if N14 has picked up a more descriptive name/symbol since you *F submitted the abstract then this would be a good time to tell me so I *F can get it into the database. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From hanyu@sakura.cc.tsukuba.ac.jp Wed Mar 27 00:48:08 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10498 *F Dear Rachel, *F We have mapped N14 to 45D. *F We have not determined yet which of genes within this region is N14. *F Best regards, *F Kazuko Hanyu # *U FBrf0146821 *a Clendening *b B. *t 2002.3.23 *T personal communication to FlyBase *u *F From biobzc@mail1.hofstra.edu Sat Mar 23 21:55:03 2002 *F From: Beverly Clendening *F Subject: Re: Helping FlyBase: ADRC-10520 *F To: rd120@gen.cam.ac.uk *F While you are at it BC-1 at 53E can be removed from the database. We *F discovered this year that the mutant phenotype was unrelated to the P-element *F insertion. Thanks. *F Beverly Clendening *F From rd120@gen.cam.ac.uk Wed Mar 27 11:03:22 2002 *F To: biobzc@Mail1.Hofstra.edu *F Subject: Re: Helping FlyBase: ADRC-10520 *F Hi Beverly, *F Thanks for this additional piece of info. I've included what we have *F for BC1 below, in abbreviated form. Seems to me that the existence of *F the allele is still valid, i.e. there still is a BC1 mutant that is *F short lived, but that the link to the P{lacZ} should be removed. I *F will keep a record of the BC1 insertion under the new name P{lacZ}BC1, *F with the map location 53E. Presumably the BC1 mutant phenotype stll *F maps to the second chromosome. If I have got any of this wrong please *F correct me, otherwise I'll go ahead and make these changes. *F Best regards, *F Rachel. # *U FBrf0146822 *a Yu *b J. *t 2002.3.29 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:45:50 2002 *F To: jy64@cornell.edu *F Subject: Helping FlyBase: ADRC-10180 *F Dear Jiangtao, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Greatwall, a putative protein kinase required for chromosome *F condensation and mitotic progression in Drosophila.' *F You mention a gene that is new to FlyBase, gwl. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jy64@cornell.edu Fri Mar 29 05:55:13 2002 *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10180 *F Rachel, *F The greatwall gene is actually corresponding to CG7719 in flybase, which has *F been allocated a name of pk91c. But it means nothing except saying it is a *F putative kinase. I thought I put it in my abstract, but I might forget. *F Thank you for your message. *F Jiangtao *F \----- Original Message \----- # *U FBrf0146823 *a Moffat *b K. *t 2002.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Mar 31 20:24:43 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GawB}OK307 insertion site *F The following information was provided by Kevin Moffat, University of *F Warwick (3/02). *F P{GawB}OK307 maps to 50C12 approximately 2 kb distal to the STS *F sequence of P{lacW}l(2)k04204k04204. # *U FBrf0146824 *a O'Kane *b C. *t 2002.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Mar 31 20:41:01 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: O'Kane insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Cahir O'Kane, Cambridge University (2/02). *F 1. The following are homozygous viable and fertile second chromosome *F insertions: *F P{GawB}OK72 *F P{GawB}OK376 *F P{UAS-HIV-1\Rev-GFP}B2 *F P{dbe+t6.5}X1 *F P{hs-raps.P}I1 *F P{UAS-Hsap\KCNJ2.EGFP}1 *F 2. The following are homozygous viable and fertile third chromosomes *F insertions: *F P{UAS-G-salpha60A.C}9 *F P{UAS-dbe.C}L2 *F P{UAS-dbe.ATG-FLAG}A12 *F 3. P{UAS-G-salpha60A.Q215L}16 is carried on a homozygous lethal third *F chromosome: *F 4. P{GawB}OK72 and P{GawB}OK376 cause GAL4 expression in oenocytes. *F 5. P{GawB}OK307 causes GAL4 expression in the giant descending neuron circuit. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146825 *a O'Kane *b C. *t 2002.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Mar 31 20:46:43 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-amph.R}LD19810 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Cahir O'Kane, Cambridge University (2/02). *F P{UAS-amph.R}LD19810 is carried on a homozygous lethal third chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146826 *a Green *b R. *t 2002.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Mar 31 21:53:21 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: drm region mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Ryan Green in Judith Lengyel's lab, UCLA (2/02). *F Df(2L)drm-P1 and Df(2L)drm-P2 were recovered as P transposase-induced *F deletions involving P{lacW}Pdsw[k10101]. In both deletions, the P element *F insertion is retained, but a chromosomal region flanking the proximal side *F of the insertion is deleted. The proximal breakpoint of Df(2L)drm-P2 lies *F within the for gene, so the inferred cytological breakpoints of the *F deletion are 23F3-4;24A1-2. The proximal breakpoint of Df(2R)drmP1 lies *F between drm and sob, so the inferred cytological breakpoints of the *F deletion are 23F3-4;24A1. *F The distal ends of Df(2L)drm-P1 and Df(2L)drm-P2 overlap *F Df(2L)tim-02. The proximal end of Df(2L)drm-P2 overlaps Df(2L)ed1. *F An F2 screen for EMS-induced lethal mutations that failed to complement *F Df(2L)drm-P2 produced the following mutations that lie outside Df(2L)drm-P1: *F l(2)24Aa1-9 *F l(2)24Ab1-10 *F l(2)24Ac5-25 *F l(2)24Ad5-58 *F l(2)24Ae5-38 *F l(2)24Ae5-77 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146827 *a Deal-Herr *b M. *c K. *d Cook *t 2002.4.1 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 01 18:42:12 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC18 *F Isolation and characterization of Df(2L)BSC18 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC18 was isolated as a P transposase-induced male recombination *F event involving P{lacW}mamk02214 and P{EP}EP993. The deletion was *F isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 *F females X cn1 P{EP}EP993/P{lacW}mamk02214 bw1 sp1; TMS, Sb1 *F P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints *F 50D1;50D2-7. Df(2R)BSC18 failed to complement cg1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146828 *a Levis *b R. *t 2002.4.4 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Thu Apr 04 20:46:03 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: Merge CG11140 \- Aldh-III? *F It appears to me that CG11140 and Aldh-III are synonyms. The FlyBase *F gene report for CG11140 lists no synonyms and the report for Aldh-III *F does not list CG11140 as a synonym. *F Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0146829 *a Younger *b S. *t 2002.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Apr 18 21:13:53 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{ato-GAL4.3.6}14a insertion *F The following information accompanied stocks from Susan Younger, University *F of California at San Francisco (2/02). *F P{ato-GAL4.3.6}14a is a homozygous viable and fertile second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146830 *a Christensen *b T. *t 2002.4.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Apr 22 15:32:56 2002 *F To: Calvi@mail.med.upenn.edu *F Subject: Helping FlyBase: ADRC-1012A *F Dear Brian, *F Please feel free to pass this on to first author T. Christensen \- who is *F not in the FlyBase people list so I couldn't address it to them myself. *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'DmMcm10 interacts with members of the Pre-RC'. *F You mention a gene that is new to FlyBase, Mcm10. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help. *F Best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From twc4@cornell.edu Mon Apr 22 17:38:52 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Mcm10 *F Rachel, *F Thanks for your email, it got to me through Brian Clavi. Mcm10 corresponds *F to CG9241. Although Flybase reports the incorrect AA length. I've determine *F experimentally that the protein is 776AA with a MW of 86.5 kD.... *F Thanks--Tim # *U FBrf0146831 *a Poole *b S. *t 2002.4.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Apr 22 15:31:50 2002 *F To: jmerriam@biology.ucla.edu *F Subject: Helping FlyBase: ADRC-1006A *F Dear John, *F Please feel free to pass this on to first author P. Ohmstedt \- who is *F not in the FlyBase people list so I couldn't address it to them myself. *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Does coronin, a putative actiun binding protein, influence formation *F of actin bundles?'. *F You mention a gene that is new to FlyBase, coronin. Do you know which *F of the Genome Project CG annotations your gene corresponds to? Is it *F by any chance CG9446? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Thank you very much for your help. *F Best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From poole@lifesci.ucsb.edu Mon Apr 22 19:58:09 2002 *F To: rd120@gen.cam.ac.uk, John Merriam *F Subject: Re: Helping FlyBase: ADRC-1006A *F Hello Rachel, *F Yes, it is CG9446. *F Stephen Poole # *U FBrf0146832 *a Boswell *b R. *t 2002.4.19 *T personal communication to FlyBase *u *F >From kcook@bio.indiana.edu Fri Apr 19 02:05:37 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Boswell complementation groups *F Rachel-- *F I have received stocks from Bob Boswell prior to the publication of a paper *F in Genetics describing their creation. The following loci and alleles will *F be described in the paper *F l(2)44Fl1 *F l(2)44Fm2 *F l(2)44Fn1 *F l(2)44Fo1 *F l(2)45An1 *F l(2)45Ao1 *F l(2)45Ap1 *F l(2)45Aq1 *F l(2)45As1 *F cri1 *F In addition, Bob and colleagues describe a locus l(2)44Fk with at least one *F allele (l(2)44Fk1). Unfortunately, the name l(2)44Fk was assigned to a *F complementation group from Bour et al. 2000 (FBrf0128410) after they *F submitted the manuscript. It would cause the least confusion to change *F Boswell's l(2)44Fk to l(2)44Fp and list l(2)44Fk as a synonym in the *F l(2)44Fp FlyBase entry. *F ... *F Best regards, *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146833 *a Robertson *b H. *t 2002.4.16 *T personal communication to FlyBase *u *F From hughrobe@life.uiuc.edu Tue Apr 16 18:25:37 2002 *F Subject: Mad/Mnt in Drosophila *F To: Eisenman_Robert *F cc: Drysdale_Rachel *F Dear Dr. Eisenman, *F At the Drosophila conference in San Diego last week I was interested in two *F posters from your laboratory describing a homolog of the vertebrate MAD *F proteins in Drosophila. I talked with one of the presenters, so maybe she has *F alerted you to this issue. *F I wonder if your Drosophila protein is essentially the same as *F GH28809p/EG:114E2. If so I believe this is in fact the ortholog of the *F vertebrate MNT protein. I believe this because I have undertaken molecular *F phylogenetic analyses of these two proteins, MAD and MNT, to determine why a *F MAD ortholog we have detected as an EST in honey bees is not in the Drosophila *F genome. It is also missing from the mosquito Anopheles gambiae genome that *F recently became available, so was apparently lost near the origin of the order *F Diptera. Instead both flies have clear orthologs of MNT. This is true both by *F sequence comparisons of the alignable regions, plus the Drosophila *F GJ28809p/EG:114E2 proteins have a ±200aa N-terminal region that fits better *F with it being MNT than being MAD (although the annotation of this N-terminus *F is not clear so maybe you can help clear that up \- there seem to be two *F isoforms with two different N-termini, with ESTs for both \- perhaps these two *F isoforms effectively function as both MNT and MAD). *F In fact last night I came across a paper by Peyrefitte et al in Mechanisms of *F Development last year (104; 99-104) noting that Drosophila MAD is missing and *F that the above gene is the apparent ortholog of vertebrate MNT (I'll copy this *F message to Rachel Drysdale of FYBASE in Cambridge, asking why this gene is not *F yet annotated as Mnt in FLYBASE). *F I think using the name to MNT would be better to avoid any confusion in the *F future on this issue. In addition this would avoid confusion with the already *F named Drosophila gene Mad (Mothers against dpp), which has precedence. *F In case you are interested, below is the full-length aa sequence for the bee *F MAD ortholog. *F MSIAALLQAAEYIERREREAEHGYASTMPMPDDMRTVTKRPKTKKSQGSRTTHNELEKNRRAHLRNCLEKLKVLVPLG *F PETSRHTTLGLLTKAKRFIKSLEERERKHAVHKEQLSREQRFLRRRLEQLTNQTGLHGLHGLHGLHGLSSSAPTGSSC *F GPGAAAAAALLSKRRSVSECSLGTASSTSSTASSRNSDRSAGSPSVSESDEVDVIGYTSNQSDTDDHSSVQSSSDSGV *F TMSTSRLTLSEMMDNL *F Hugh *F From rd120 Wed Apr 24 10:09:11 2002 *F To: hughrobe@uiuc.edu, eisenman@fhcrc.org *F Subject: Re: Mad/Mnt in Drosophila *F Cc: rd120@gen.cam.ac.uk *F Hi Hugh, *F >(I'll copy this *F >message to Rachel Drysdale of FYBASE in Cambridge, asking why this gene is not *F >yet annotated as Mnt in FLYBASE) *F When we curated *F FBrf0137013 == Peyrefitte et al., 2001, Mech. Dev. 104(1-2): 99--104 *F we noted a mention of EG:114E2, and that the authors referred to it at *F least once as DmMnt, but did not rename it Mnt. *F It seems to me, based on what you have written and what else we have in *F the gene record for EG:114E2, that there is a good case for renaming *F FBgn0023215 Mnt, and I will see to it that the symbol gets changed in *F our files. It is the usual practise to overwrite the anonymous genome *F project type symbols (such as EG:114E2) with more biological names *F when those appear in the literature, as in FBrf0137013. It was *F probably just a lapse of concentration that meant that we missed this one. *F Rachel. *F From hughrobe@life.uiuc.edu Wed Apr 24 17:43:01 2002 *F Subject: Re: Mad/Mnt in Drosophila *F To: Rachel Drysdale *F Rachel, if you want to include a reference that my phylogenetic analyses *F indicate that it is the ortholog of vertebrate MNT that's fine with me. *F Hugh M. Robertson, Professor *F Department of Entomology, University of Illinois at Urbana-Champaign *F 320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801 *F Phone 217-333-0489; FAX 217-244-3499; email hughrobe@uiuc.edu *F WWW http://www.life.uiuc.edu/robertson/lab.html # *U FBrf0146834 *a Young *b M. *t 2002.4.8 *T personal communication to FlyBase *u *F From young@mail.rockefeller.edu Mon Apr 08 23:14:31 2002 *F Subject: Re: flea element *F To: Michael Ashburner (Genetics) *F Dear Michael, *F Here is a consensus sequence for the flea LTR: *F GTAAGATTGTTTATTATTATTGTTTATTATTAATTTAATTATTAGTTTAATTCTAAATGCGGTAATTATATAAAGTATT *F CTTAGTTTGTGACCGAACGACTGCTGCTGAGAACTCGACTGACTGCAACGCTGACTTCCACTCGACTCTACTGCTGCCT *F TGCGATCGTTCCTTCGATGACTGATTGTCGATAACTTCGATTTCCGACAATCATGTTGCTGCTCAACAGCGCTGCCAAC *F CGGGTTGTTGCCAGCCTTCAGCCTTACTCCATGTTAC *F Aside from that, Simon sends the info below. Hope these help. *F Great seeing you in NY! *F Best, *F Mike *F This comment seems to be based on a high stringency southern blot  in *F Genet. Res. 1994 64(3):167--181  by Ding & Lipshitz showing cross *F hybridisation between flea  and kermit. As far as I can tell there is no *F sequence data for Kermit so I cannot tell if it is the same as flea. The *F restriction map of flea and the ltr sequence match the micropia sequence *F here: *F LOCUS       DMBLPP                  5416 bp    DNA     linear   INV *F 22-NOV-1996 *F DEFINITION  D.melanogaster (Oregon R) gene for blastopia polyprotein. *F ACCESSION   Z27119 *F VERSION     Z27119.1  GI:415797 *F KEYWORDS    blastopia polyprotein; micropia-like element. *F SOURCE      fruit fly. *F   ORGANISM  Drosophila melanogaster *F             Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta; *F             Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; *F             Muscomorpha; Ephydroidea; Drosophilidae; Drosophila. *F REFERENCE   1  (bases 1 to 5416) *F   AUTHORS   Frommer,G., Schuh,R. and Jackle,H. *F   TITLE     Localized expression of a novel micropia-like element in the *F             blastoderm of Drosophila melanogaster is dependent on the *F anterior *F             morphogen bicoid *F   JOURNAL   Chromosoma 103 (2), 82-89 (1994) *F   MEDLINE   94333069 *F REFERENCE   2  (bases 1 to 5416) *F   AUTHORS   Frommer,G.G. *F   TITLE     Direct Submission *F   JOURNAL   Submitted (09-NOV-1993) Gvtz G Frommer, Molekulare *F             Entwicklungsbiologie, Max-Planck-Institut fur *F biophysikalische *F             Chemie, Am Fassberg, Gvttingen, 37018, Germany *F Simon *F On Friday, April 5, 2002, at 03:51 AM, Michael Ashburner (Genetics) *F wrote: *F > Dear Mike *F > *F > I am trying to clear up some old transposable elements in Drosophila *F > which have never had public sequence data. *F > *F > This is part of a collaboration with Josh Kaminker and Casey Bergman in *F > Gerry's *F > group; we are preparing a paper describing all of the elements in *F > Release *F > 3 of the sequence. *F > *F > Do you have any sequence data for flea ? Or even *F > high resolution restriction maps ? Or do you know these to be an element *F > called by another name ? Was the relationship between flea and Kermit *F > ever worked out ? *F > *F > Best *F > *F > *F > Michael *F > *F >>b========================================================== *F OK *F BLASTN 2.0MP-WashU 13-Sep-2001 sol8-ultra-ILP32F64 22:11:38 13-Sep-2001 *F Copyright (C) 1996-2001 Washington University, Saint Louis, Missouri USA. *F All Rights Reserved. *F Reference: Gish, W. (1996-2001) http://blast.wustl.edu *F Notice: this program and its default parameter settings are optimized to find *F nearly identical sequences rapidly. To identify weak similarities encoded in *F nucleic acid, use BLASTX, TBLASTN or TBLASTX. *F Query= flea, 274 bases, 94CD2A55 checksum. *F (274 letters) *F Database: /data/blast/db/na_te.dros *F 87 sequences; 436,390 total letters. *F Searching....10....20....30....40....50....60....70....80....90....100% done *F Smallest *F Sum *F High Probability *F Sequences producing High-scoring Segment Pairs: Score P(N) N *F gb|Z27119|blastopia DMBLPP 5034bp Derived from Z27119 (g4... 1063 1.6e-44 1 *F >gb|Z27119|blastopia DMBLPP 5034bp Derived from Z27119 (g415797) (Rel. 50, Last *F Length = 5034 *F Minus Strand HSPs: *F Score = 1063 (165.5 bits), Expect = 1.6e-44, P = 1.6e-44 *F Identities = 219/224 (97%), Positives = 219/224 (97%), Strand = Minus / Plus *F Query: 274 GTAACATGGAGTAAGGCTGAAGGCTGGCAACAACCCGGTTGGCAGCGCTGTTGAGCAGCA 215 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4758 GTAACATGGAGTAAGGCTGAAGGCTGGCAACAACCCGGTTGGCAGCGCTGTTGAGCAGCA 4817 *F Query: 214 ACATGATTGTCGGAAATCGAAGTTATCGACAATCAGTCATCGAAGGAACGATCGCAAGGC 155 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4818 ACATGATTGTCGGAAATCGAAGTTATCGACAATCAGTCATCGAAGGAACGATCGCAAGGC 4877 *F Query: 154 AGCAGTAGAGTCG-AGTGGAAGTCAGCGTTGCAGTCAGTCGAGTTCTCAGCAGCAGT-CG 97 *F |||||| |||| | ||||||||||||||||||||||||||| ||||||||||||||| || *F Sbjct: 4878 AGCAGTGGAGTAGGAGTGGAAGTCAGCGTTGCAGTCAGTCGTGTTCTCAGCAGCAGTTCG 4937 *F Query: 96 TTCGGTCACAAACTAAGAATACTTTATATAATTACCGCATTTAG 53 *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4938 TTCGGTCACAAACTAAGAATACTTTATATAATTACCGCATTTAG 4981 *F Score = 1027 (160.1 bits), Expect = 7.0e-43, P = 7.0e-43 *F Identities = 217/224 (96%), Positives = 217/224 (96%), Strand = Minus / Plus *F Query: 274 GTAACATGGAGTAAGGCTGAAGGCTGGCAACAACCCGGTTGGCAGCGCTGTTGAGCAGCA 215 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2 GTAACATG-AGTAAGGCTGAAGGCTGGCAACAACCCGGTTGGCAGCGCTGTTGAGCAGCA 60 *F Query: 214 ACATGATTGTCGGAAATCGAAGTTATCGACAATCAGTCATCGAAGGAACGATCGCAAGGC 155 *F |||||||||||||||||| |||||||||||||||||||||||||||| |||||||| ||| *F Sbjct: 61 ACATGATTGTCGGAAATCCAAGTTATCGACAATCAGTCATCGAAGGA-CGATCGCA-GGC 118 *F Query: 154 AGCAGTAGAGTCGAGTGGAAGTCAGCGTTGCAGTCAGTCGAGTTCTCAGCAGCAGTCGTT 95 *F |||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 119 AGCAGTAGAGGCGAGTGGAAGTCAGCGTTGCAGTCAGTCGAGTTCTCAGCAGCAGTCGTT 178 *F Query: 94 CGGTC-ACAAACTAAGAA-TACTTTATATAATTACCGCATTTAG 53 *F ||||| |||||||||||| ||||||||||||||||||||||||| *F Sbjct: 179 CGGTCCACAAACTAAGAAATACTTTATATAATTACCGCATTTAG 222 *F Parameters: *F B=50 *F V=100 *F sort_by_pvalue *F filter=dust *F sump *F ctxfactor=2.00 *F E=10 *F Query \----- As Used \----- \----- Computed *F \---- *F Strand MatID Matrix name Lambda K H Lambda K H *F \+1 0 \+5,-4 0.192 0.173 0.357 same same same *F Q=10,R=10 0.104 0.0151 0.0600 n/a n/a n/a *F \-1 0 \+5,-4 0.192 0.173 0.357 same same same *F Q=10,R=10 0.104 0.0151 0.0600 n/a n/a n/a *F Query *F Strand MatID Length Eff.Length E S W T X E2 S2 *F \+1 0 274 227 10. 106 11 n/a 73 0.022 74 *F 79 0.023 99 *F \-1 0 274 227 10. 106 11 n/a 73 0.022 74 *F 79 0.023 99 *F Statistics: *F Database: /data/blast/db/na_te.dros *F Title: /data/blast/db/na_te.dros *F Posted: 2:25:51 PM PST Mar 20, 2002 *F Format: BLAST-1.4 *F \# of letters in database: 436,390 *F \# of sequences in database: 87 *F \# of database sequences satisfying E: 1 *F No. of states in DFA: 189 (189 KB) *F Total size of DFA: 200 KB (2054 KB) *F Time to generate neighborhood: 0.02u 0.12s 0.14t Elapsed: 00:00:01 *F No. of threads or processors used: 4 *F Search cpu time: 0.05u 0.06s 0.11t Elapsed: 00:00:00 *F Total cpu time: 0.09u 0.22s 0.31t Elapsed: 00:00:01 *F Start: Wed Apr 17 09:51:30 2002 End: Wed Apr 17 09:51:31 2002 # *U FBrf0146835 *a Czank *b A. *t 2002.4.24 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Wed Apr 24 14:32:50 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail *F comments: sarah and nebula (genetic symbol: nla) are allelic and share the *F same mRNA *F mailto: flybase-help@morgan.harvard.edu *F realname: Andreas Czank *F reply-to: andreas.czank@freesurf.ch *F Sent from computer fwigk1.admin.ch # *U FBrf0146836 *a Caggese *b C. *t 2002.4.6 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 06 10:23:29 2002 *F Date: Sat, 6 Apr 2002 01:23:20 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG11079 on Sat Apr 6 01:23:20 2002 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG11079 *F Release: 2 *F Gene annotation error *F Gene CG11079 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >, 1312 bp. *F agcagattcagtaggtttcgcgatttggagagcagttgcgtaagattctagaaccgtttt *F cgaatcgctttgcaggcacttgaagatgtcgcagatcggagaactgggcagtggagccgg *F caacggcggcggcggcggcggatccatccgggaggcgggcggttcatttggcaaaatgga *F ggctgctcgcgaggaggagttcttctacaagcagcaaaaggagcaactgaagaacctgaa *F gaccaagacggagcctaaggcaccagaggctcccaagaagtgagcccaaccaggagcttt *F gaactccactagcttaactatggttaggctggattgtctgaattgtatttaatgggaatg *F gtactccaaatatattttgtttaatttacaactggttgtgtattggataactcccatccc *F tgatcagctgatcgccccgcggctcacccaaactgcaagcctaaactttccccagaccgc *F tccacttccatttgtttacgaacagcgctgcaaagcggaacagccgacagataagcgtca *F gtgtaagcgcagctgataacgggcggcggagtggcgacctaaagacgcatggaccgcgca *F ggcagatggaaacagttcgcaccggttcgctcgagtgtgcagtagatgatccagcggcag *F gaatggcggccacgatccagaacaccctgaaggtggcgctgcgaaagcgcatgaaggatg *F cactgaagggcatcgacgcggaggccatcgcccggcagtcgcaggccgtcacggctaagg *F tgctgcaaagcgagatcttccggcaggcgcagcgggtgagcatttacctgagcacagcct *F cggagctggacaccacggcgctgctgtcggagatgttccgcctggagaagatggtctttg *F tgcccacctacgagggcagcaggatgaagatggtgcggctgcgcggcatggaggagtacg *F agagcctgcctctgaccaagtggaacataaagcagccggacttcaaggaggcacgcgagg *F atgccatgaccaacgggcacggcatcgatctcttcattgtgcccggtgtggccttcaccc *F gctgcggagctcggatgggccatggcatgggctactacgacaagttcctcaagcagcacg *F cggagaagtatccgcacaagaagatctcgctgatggcactgtcgctcaacgagcagatag *F tcagcaacgaggagttgcccatggagtcgcacgacgtccgtttgcacagtgtaattacgg *F aaaactaacttttggcttacatacaaagtgggaagtaaagcgaactaatacg *F Protein sequence: *F >, 220 aa. *F METVRTGSLECAVDDPAAGMAATIQNTLKVALRKRMKDALKGIDAEAIARQSQAVTAKVL *F QSEIFRQAQRVSIYLSTASELDTTALLSEMFRLEKMVFVPTYEGSRMKMVRLRGMEEYES *F LPLTKWNIKQPDFKEAREDAMTNGHGIDLFIVPGVAFTRCGARMGHGMGYYDKFLKQHAE *F KYPHKKISLMALSLNEQIVSNEELPMESHDVRLHSVITEN *F Comments: EST clot: *F >GH24554.5prime AI404737 *F >GH17091.5prime AI386817 *F >SD20520.5prime BI638273 *F >RH09315.3prime BI573808 # *U FBrf0146837 *a Frederic *b P. *t 2002.4.5 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Fri Apr 05 16:22:30 2002 *F Date: Fri, 5 Apr 2002 07:22:31 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: frederic.prince@unibas.ch *F Subject: FlyBase error report for CG12510 on Fri Apr 5 07:22:31 2002 *F Error report from Prince Frederic (frederic.prince@unibas.ch) *F Gene or accession: CG12510 *F Release: 2 *F Missed gene *F Comments: The gene reported as doe (downstream of eyeless) is the same than *F sidestep. *F Doe is reported as an abstract of the European Droso. research conference. # *U FBrf0146838 *a Robin *b C. *t 2002.4.10 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 11 05:11:53 2002 *F Date: Wed, 10 Apr 2002 21:11:42 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: crobin@unimelb.edu.au *F Subject: FlyBase error report for CG15100 on Wed Apr 10 21:11:42 2002 *F Error report from Charles Robin (crobin@unimelb.edu.au) *F Gene or accession: CG15100 *F Release: 2 *F Gene annotation error *F Gene CG15100 has incorrect exon/intron structure or translation start site. *F Protein sequence: *F MVNAKYKDLLVLPTLELDNGLRLFSPAAIAKYLFVGKGQQRDEWLEWSATLLAPALAHHMAVGHKADANALPVLNALVK *F KLDDKLKATPYLAGDKPSAADIAIWSLLAPDGTLKGAQNVDNLRDWYGRVKALPEVQEVLAEQPLKDLSFNALQQSNRY *F GGLHHVPLKRLSLADASKLLVDTTPTVADTVTNEEISAAKAAFTYTAPKEIKEERTVLPKPGERNVLITSALPYVNNVP *F HLGNIIGCVLSADIYARYSRSAGYNTLLICGTDEYGTATENKALAENLTPREICDKYFELHNAIYRWFGIGFDYFGRTT *F TQEQTDIVQEAFKDVLKAGYIITESVEQLLCQKCDRFLADRFVEGTCPHPGCGYEDARGDQCDKCGKLVNATELIRPRC *F KVCNSAPVLRSSDQLFIDLPKAEPQLKEWVDKSEGGWTHNAKVITRAWLKEGLKPRCITRDLKWGIPVPHEGFEKKVFY *F VWFDAPFGYVSMTKRYTKEYQQWWQPAKGTDVELFQFMAKDNVPFHSVVWPSVLLAINKGHTLVSHIMATEYLNYEDGK *F FSKSRGIGVFGNDAQETGIPADVWRFYLASARPEGQDSSFSWNDLAARNNSELLNNLGNFVNRALVFCEKNFSSTVPGV *F ITTQDELVLLALINRELRGYINSMEKAKLRDGVRHLLAISRHGNGYMQSQQPWVLLKGTDDQKTRASTIIGLCVNIACL *F LANLLFPYMPTTARTLFGQLNAKQTPLNAEKPLVTLLLPAGHQIGKPAPLFAKLEQSFIDELKGKYGGAQATNDAAHSQ *F ISAADLEKAVQAQADKVRELKASTKDKAIWQPEVTKLLDLKKQLEEAKKKTATAAAPAATPAPSNGSQSVQDLEKAIQE *F QGDKVRKLKGSTKDKTVWQPEVNILLDLKKQLEAVQKAAKAAPAANAAPAASPAATADAAKVKALEDKIAQQAEKVRTL *F KATGDAAVWKPEVDILLSLKNELAALTGTPVAGGQGKGKKKK *F Comments: The 'translation fasta' is truncated relative to the amino acid *F sequence that would be inferred from the 'gene region fasta'. The correct *F inferred sequence is 990 amino acids not the 37 currently shown. # *U FBrf0146839 *a Caggese *b C. *t 2002.4.11 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Thu Apr 11 12:28:03 2002 *F Date: Thu, 11 Apr 2002 04:28:04 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG1673 on Thu Apr 11 04:28:04 2002 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG1673 *F Release: 2 *F Gene annotation error *F Gene CG1673 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F > *F TTTCTTGCAGAGACTTCGTGCCGTGAAGATCGAACTGAGAAAAGAAAAAGAGCAGAAGCA *F GAAGGGCTGTAGAAAGAAAAAAAGAAAGTAAATCCATACACCGAAATAAAACACAACTGC *F GTGCTTTAAGAATTTTTTTTTTACTGTGCAAAGTGCAGCAGTTCCTTGCTCATTAAACTA *F GAACCAAGAAATACAATATTTAACGCGTCAGTTCGCGTAAGTGCGGCAAAGTGCGAGTGC *F AAGAGACGCTAAACGAGCGATACAAAAAAAAAAAAAAACGGAAATTTCGTAGAGCAGCAA *F AGCGAGTCCAAATCCAAAAGTTTCTGAAAGTAACGAGACAGAAGATTTCGCAGCCAAATT *F CAACAAGATGACGACCAAAATGGCAATTAACGCAAAGGATATTTTCCGCAATCAGCATCG *F CCTGATCCGCTTCCTCGAGCAGTCGATCCGGTTGTGCAGCAACCAGTCTCTGAAAGCCGC *F CCAAGCGGCACAGTTGCAACAGAAATCGTCGGCGGACTTCGAGATCACCGCCTCGCTGGA *F CGCCCAATACACCAGTGCCCCGGAGCCCATTAAGCATGACAAGAATCTGGGTCATCAGTT *F CCGTGCCTCACAGATTTCCGTGCGACTGGCCGCACCGGAGCAACTGCAGCCCAAGCCGGA *F CAACGATGAGGAATTGGGTTTCGGCCGCCTATTCACCGATCACATGCTCAAGATCTACTA *F CCACAAGAGTTTGGGCGGATGGCAGCGACCGGAGATTACGCCGCTGGAGAATCTAGTGAT *F GCATCCCGCCGCCAAGGTCCTGCACTACGCCGTTGAGCTCTTTGAGGGCATGAAGGCGTA *F CCGCGGCGTCGATGGCAAGATCCGCATCTTCCGGCCGGACATGAACATGAACCGCATGAA *F CCTGGCCGCCCAGCGATCCGGTCTGCCCACCTTCGAGGGCAAGGAGTTCGTCCAGTGCCT *F GTCCCGCCTGCTGTCCATTGATTCCGAGTGGGTTCCTCACACGGACACCGCCAGCTTGTA *F CATCCGCCCCACACTGATCGGCATTGATCCCACACTGGGAGTTGCTTCCTCCGACTCGGC *F CCTGCTCTACACGATCCTCAGCCCCGTGGGCAGTTACTTTAAGACAGGCTCGTCCGGTGC *F CGTCTCCCTGCTGGCCGATCCCAGTTATGTTCGTGCCTGGCCAGGCGGCGTGGGCAATCG *F CAAAATGGGCTCCAACTACGCACCCACAATCAATGTGCAGAAGGAGGCGGCCGCCAAGGG *F ACTGCAGCAGGTGCTGTGGCTCTACGGCGAGGATCATCAGCTAACCGAAGTGGGCACCAT *F GAACATCTTTATGTTCTTCGTGAACGATCAAGGAGAACAAGAACTGGTTACCCCACCGCT *F GAGCGGTCTAATCCTGCCCGGTATCACCCGCGACTCCATCCTGCGCATGACCCGCCAGTG *F GGGCAAGTTTAAGGTTAGCGAGGCGAACATCACCATGCCCATGGTCTGTGAGCTCCTCAA *F CCAGGGAAGGGTAACTGCTGGAGCTCTTTGGTGCGGGAACGGCGTGCGTGGTTAGCCCCG *F TGAACAGGATCAGCTACCTTGGCCAGGATCTGTATATACCCACCATGGAGCAGGAGAAGC *F CCGTTCACGAGCTGATCCGCGAAACGCTCACCGACATTCAGTACGGTCGGGTGGATCATC *F CGTGGGCGGTGGTGATCGACTAAGTCGATCTCCCAGCCGATCCCAGCCAGAAAGCATCCA *F ACCCCAGAGCTGTTACCCTTTCCCGTTACCTTACCGCATCCCTTTCCCGGTACCGTGTAT *F TAGTAGGATTTAGTGCCAAGTGTTTTGTGGGTCGCCAATGTGCTCGAATTTGTTTCGTTT *F ATTGTGCATGTGTCTATATGCTTGCATATATCGGATATGTACAATATATATATATATATA *F TA *F Protein sequence: *F > *F MTTKMAINAKDIFRNQHRLIRFLEQSIRLCSNQSLKAAQAAQLQQKSSADFEITASLDAQ *F YTSAPEPIKHDKNLGHQFRASQISVRLAAPEQLQPKPDNDEELGFGRLFTDHMLKIYYHK *F SLGGWQRPEITPLENLVMHPAAKVLHYAVELFEGMKAYRGVDGKIRIFRPDMNMNRMNLA *F AQRSGLPTFEGKEFVQCLSRLLSIDSEWVPHTDTASLYIRPTLIGIDPTLGVASSDSALL *F YTILSPVGSYFKTGSSGAVSLLADPSYVRAWPGGVGNRKMGSNYAPTINVQKEAAAKGLQ *F QVLWLYGEDHQLTEVGTMNIFMFFVNDQGEQELVTPPLSGLILPGITRDSILRMTRQWGK *F FKVSEANITMPMVCELLNQGRVTAGALWCGNGVRG *F Comments: EST RE68592.5prime identifies a new 5' exon in the CG1673 gene # *U FBrf0146840 *a Hazelett *b D. *t 2002.4.8 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 08 23:13:25 2002 *F Date: Mon, 8 Apr 2002 15:13:22 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: hazelett@uoneuro.uoregon.edu *F Subject: FlyBase error report for CG1915 on Mon Apr 8 15:13:22 2002 *F Error report from Dennis Hazelett (hazelett@uoneuro.uoregon.edu) *F Gene or accession: CG1915 *F Release: 2 *F Gene annotation error *F Genes CG1915 and CG18857 should be merged. *F Comments: These two correspond to Drosophila Titin # *U FBrf0146841 *a Caggese *b C. *t 2002.4.15 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 15 18:34:43 2002 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 Apr 2002 10:34:39 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG2139 on Mon Apr 15 10:34:39 2002 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG2139 *F Release: 2 *F cDNA or EST error *F Gene cDNA sequence: *F >CT6980-cDNA_sequence from_gene:CG2139/aralar1 *F AGAATGGAAAAGTAGCGCGACGCCAGCGGATGACAGCCGAGAAAATCGCGCGCGTCCCTC *F AGATATAAAAATCACAAGTTGGCAATAAATATATCCGGTTCGGGCAGTGAAAGTATATTA *F TTTATACTAACCAGCAAGCCTTTAAAGTATTGAAAAAGCCACAACGACTCGCGAGTGATC *F ATTAAAAGCATATTTAACCAATAGTTAGATCAAGTTAAGCCGAAGTCTCCGATAAACGAA *F CACTCAAACAGATAGTGCGAACGTGCCTGAATTTCGCAAAGATTCACAGACTTCAATTGT *F GTTCATACGTATAAGTGAATATGCACGATAAGGATGAGTCTCCGTCGCTTTTGAAGCGCG *F CCGGCACCGAAAAGTTGCGAGAGGTGTTCCTGAAGTACGCATCCATCCAGAAGAATGGCG *F AGCACTATATGACCAGCGAGGACTTCGTGCGAAAGTTCCTGGGACTCTTTTCCGAATCTG *F CATTTAATGACGAATCGGTGCGCCTGCTGGCCAACATTGCGGACACGAGCAAGGATGGAC *F TCATCTCCTTCTCGGAGTTCCAGGCGTTCGAGGGTCTGCTCTGCACTCCAGATGCCCTCT *F ACAGGACCGCTTTCCAGCTGTTCGACCGCAAGGGCAACGGCACTGTTTCCTATGCTGACT *F TCGCTGATGTCGTACAAAAAACTGAACTGCACTCAAAGATACCTTTTAGCTTAGATGGCC *F CCTTTATCAAGCGCTACTTTGGTGATAAGAAGCAACGCCTTATCAACTATGCTGAGTTCA *F CGCAGCTGCTGCACGACTTTCACGAGGAGCACGCGATGGAGGCCTTCCGCAGCAAGGATC *F CAGCTGGCACTGGGTTCATCTCGCCATTGGACTTCCAGGACATCATAGTTAATGTTAAGC *F GGCATTTACTGACCCCCGGCGTCAGGGACAATCTAGTTTCGGTAACTGAGGGTCACAAAG *F TGAGCTTCCCGTATTTCATCGCATTTACCTCGCTGCTGAACAACATGGAACTGATCAAAC *F AAGTCTACCTGCACGCCACCGAGGGCAGTCGCACGGATATGATCACCAAGGATCAGATCC *F TCCTCGCCGCCCAAACGATGAGCCAAATCACGCCGCTGGAGATCGACATACTTTTCCACC *F TGGCGGGCGCCGTCCATCAAGCAGGCCGCATCGACTATAGTGACCTGAGCAACATCGCAC *F CCGAGCATTACACCAAGCATATGACACATCGTCTGGCGGAGATCAAGGCGGTGGAGAGTC *F CTGCGGACCGATCTGCCTTTATTCAGGTCCTGGAGAGCTCTTACCGTTTCACTCTCGGTT *F CCTTTGCCGGCGCTGTGGGCGCCACTGTGGTCTACCCCATCGATCTAGTCAAGACTCGAA *F TGCAGAACCAGCGAGCGGGTTCCTATATTGGTGAAGTTGCCTATCGAAACTCATGGGACT *F GCTTCAAAAAGGTGGTTCGCCACGAGGGATTCATGGGTCTCTACAGAGGTCTCCTGCCCC *F AGCTAATGGGCGTGGCCCCCGAGAAAGCCATCAAACTGACGGTGAATGATTTGGTGAGGG *F ACAAACTCACCGACAAGAAGGGCAACATTCCCACATGGGCGGAAGTTCTGGCTGGTGGAT *F GTGCCGGTGCGTCGCAGGTGGTGTTCACCAATCCCCTGGAGATTGTGAAGATCCGTCTCC *F AGGTGGCGGGAGAAATCGCTAGTGGGAGCAAGATCCGCGCGTGGTCGGTGGTCCGGGAAC *F TGGGACTCTTTGGCCTCTACAAGGGAGCCAGAGCATGTCTCCTCCGCGATGTGCCATTCT *F CGGCCATCTACTTCCCGACCTACGCCCACACCAAGGCCATGATGGCCGACAAGGACGGCT *F ACAATCATCCACTCACCCTGCTGGCCGCCGGTGCCATCGCCGGTGTTCCGGCCGCCTCCC *F TTGTCACGCCCGCCGACGTCATCAAGACCCGCCTTCAGGTGGTTGCCCGATCTGGACAGA *F CCACCTACACCGGCGTATGGGATGCCACCAAGAAGATCATGGCCGAGGAGGGACCCAGAG *F CCTTCTGGAAGGGCACAGCCGCTCGAGTGTTCCGTTCCTCACCACAGTTCGGTGTGACCC *F TGGTGACCTACGAACTGCTACAGCGCCTCTTCTACGTGGACTTCGGAGGCACTCAGCCCA *F AGGGATCCGAGGCGCACAAGATCACCACTCCCCTGGAACAGGCGGCTGCGTCGGTGACCA *F CCGAAAACGTGGACCACATTGGTGGCTACCGGGCGGCAGTTCCTCTTCTGGCGGGAGTGG *F AGTCCAAGTTCGGGTTGTATCTGCCGCGATTTGGACGCGGAGTAACCGCTGCCTCGCCCT *F CGACGGCCACGGGATCCTGATTATATTGGCGCCACCGCCTGCCAAGCGCCACGTAGTCTA *F CGGAATGCAGAAGGGATTGGCGGGATTGGGCTCCGGTGGAATTAGATATGGGATATATTT *F TTTGCTAGGACGCGTTTACACAAAATACACAAACACACAGAAGCACTGAGTATATTTTTA *F ACGAGGATCAGGGAGGAGCAGCGCCGTATCTATAGTACTATTGTATCTGAACAGCCGGGT *F TCCTGGGCACCCAGCTCTGAGCAACTGTTTTTACCACACACAGGAAAAACCTAGAGAAAA *F GTAAAGAAAAGCCCATTTGAGAGTATAGAATATCGATTTTGGCCATGGTGCGTATATATT *F TAGGCAAGTGTAGAACTGTTTTTGTAAACTTTGTTAACTAGTTTAAAGTCTAGAGCATCA *F ACCAATCACTGAACGTCAACTTTTGCACTCAATGTAACTATTAAAGCGATAAAACCTTTT *F AATCTTAGTACGAATTTGTTCAAAACAATTATCAAGCAAAGGAAATGCAATGAATGACCT *F CTTAGCTAATAATGAATAATTAAATAAAAGTCAATATTAAAAACAA *F Protein sequence: *F >pp-CT6980 from_gene:CG2139/aralar1 *F MHDKDESPSLLKRAGTEKLREVFLKYASIQKNGEHYMTSEDFVRKFLGLFSESAFNDESV *F RLLANIADTSKDGLISFSEFQAFEGLLCTPDALYRTAFQLFDRKGNGTVSYADFADVVQK *F TELHSKIPFSLDGPFIKRYFGDKKQRLINYAEFTQLLHDFHEEHAMEAFRSKDPAGTGFI *F SPLDFQDIIVNVKRHLLTPGVRDNLVSVTEGHKVSFPYFIAFTSLLNNMELIKQVYLHAT *F EGSRTDMITKDQILLAAQTMSQITPLEIDILFHLAGAVHQAGRIDYSDLSNIAPEHYTKH *F MTHRLAEIKAVESPADRSAFIQVLESSYRFTLGSFAGAVGATVVYPIDLVKTRMQNQRAG *F SYIGEVAYRNSWDCFKKVVRHEGFMGLYRGLLPQLMGVAPEKAIKLTVNDLVRDKLTDKK *F GNIPTWAEVLAGGCAGASQVVFTNPLEIVKIRLQVAGEIASGSKIRAWSVVRELGLFGLY *F KGARACLLRDVPFSAIYFPTYAHTKAMMADKDGYNHPLTLLAAGAIAGVPAASLVTPADV *F IKTRLQVVARSGQTTYTGVWDATKKIMAEEGPRAFWKGTAARVFRSSPQFGVTLVTYELL *F QRLFYVDFGGTQPKGSEAHKITTPLEQAAASVTTENVDHIGGYRAAVPLLAGVESKFGLY *F LPRFGRGVTAASPSTATGS *F Comments: There is a mistake in the translation start site. *F RE27242.5prime BI229461 extends in 5'the alt 2 mRNA. # *U FBrf0146842 *a Peifer *b M. *t 2002.4.15 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 15 20:40:34 2002 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 15 Apr 2002 12:40:31 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG4211 on Mon Apr 15 12:40:31 2002 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG4211 *F Release: 2 *F Gene annotation error *F Gene CG4211 has incorrect exon/intron structure or translation start site. *F Comments: Why does FlyBase list NonA as encoding a 230 aa protein, while *F X55902, the Genbank entry, and Q0404, the SWISS PROT entry, suggest it is more *F like 700 amino acids? # *U FBrf0146843 *a Caggese *b C. *t 2002.4.10 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Wed Apr 10 10:57:12 2002 *F Date: Wed, 10 Apr 2002 02:57:08 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG4233 on Wed Apr 10 02:57:07 2002 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG4233 *F Release: 2 *F Missed gene *F Gene cDNA sequence: *F >CG4233 alternatively transcribed mRNA *F CAACTGTGAAAAAGACATCAAAAAGTATTTTTTCTACATCAAAATTGCAAGTTTAAGTTA *F ATATTAAGTTATGAGTAGAACCATTATTATGACGCTTAAGGACATTGTGCATGTGTGGTC *F CAACGACCCGGGAGAACGGATGTGCCGAGCGAACAGAGTATCAACATGGTTCTCCGAGGT *F GCAGATGGGCCCACCCGATGCCATCTTGGGTGTCACGGAAGCCTTCAAGAAGGACACCAA *F CCCCAAGAAGATCAACTTGGGCGCTGGCGCCTATCGCGATGACAACACCCAGCCCTTCGT *F GCTCCCCAGTGTTCGGGAGGCCGAGAAGAGAGTGGTGAGCCGTAGTCTGGACAAGGAGTA *F CGCCACAATCATCGGCATTCCCGAGTTCTACAACAAGGCCATCGAGCTGGCATTGGGCAA *F GGGATCCAAGCGTTTGGCGGCCAAGCACAACGTGACCGCCCAGTCCATCAGTGGAACTGG *F AGCTCTGCGCATCGGAGCCGCCTTCCTGGCCAAGTTCTGGCAGGGCAACCGCGAGATCTA *F CATCCCGTCGCCATCGTGGGGCAACCATGTGGCCATTTTCGAGCACGCCGGTCTGCCGGT *F GAACCGATACCGCTACTACGACAAGGACACCTGTGCCCTGGACTTTGGCGGCCTGATCGA *F GGATCTGAAGAAAATCCCCGAGAAGAGCATTGTTCTTCTGCACGCCTGCGCCCACAACCC *F CACTGGAGTGGATCCCACTCTGGAGCAGTGGCGTGAGATCTCGGCTCTGGTCAAGAAACG *F CAATCTGTATCCCTTCATCGACATGGCCTACCAAGGCTTCGCCACCGGAGACATTGACCG *F CGACGCCCAGGCGGTCCGCACCTTCGAGGCCGATGGCCACGACTTCTGCCTGGCCCAGAG *F TTTCGCCAAGAACATGGGATTGTATGGTGAGCGCGCTGGCGCCTTCACCGTGCTGTGCTC *F CGACGAGGAGGAGGCTGCTCGCGTGATGTCCCAAGTTAAGATCCTGATCCGTGGTCTGTA *F CTCCAATCCCCCGGTGCACGGAGCTCGTATTGCCGCCGAGATCCTCAACAACGAGGACTT *F GCGCGCCCAGTGGCTGAAGGATGTGAAGCTGATGGCCGACCGCATCATCGATGTGCGCAC *F CAAGCTCAAGGACAATCTAATTAAGCTGGGATCCAGCCAGAACTGGGACCACATTGTCAA *F CCAAATCGGCATGTTCTGCTTCACGGGCCTGAAGCCGGAGCAGGTGCAGAAGCTGATCAA *F GGATCACAGCGTCTATCTCACCAACGATGGACGTGTTTCGATGGCGGGAGTCACCAGCAA *F GAATGTCGAGTACCTGGCTGAGAGCATACACAAGGTTACCAAGTAAGGAGGACCAGTGGA *F GATCGAAGTGGAGATGGAGTTTCTGTAGTACCTTCTAATCGGCACTTGTACGAATTTTCT *F AGCACCAACGAAATTGCGAAGTCTAGATAAGCCAATGCATTTTGCACACTCTCTATCCAT *F ATCTCTGTATATAAGCTAAATGATCTGCCTTTTAAAAAATAAAGCATTTAAAATGTTAGT *F Protein sequence: *F >translation of CG4233 alternatively transcribed mRNA *F MSRTIIMTLKDIVHVWSNDPGERMCRANRVSTWFSEVQMGPPDAILGVTEAFKKDTNPKK *F INLGAGAYRDDNTQPFVLPSVREAEKRVVSRSLDKEYATIIGIPEFYNKAIELALGKGSK *F RLAAKHNVTAQSISGTGALRIGAAFLAKFWQGNREIYIPSPSWGNHVAIFEHAGLPVNRY *F RYYDKDTCALDFGGLIEDLKKIPEKSIVLLHACAHNPTGVDPTLEQWREISALVKKRNLY *F PFIDMAYQGFATGDIDRDAQAVRTFEADGHDFCLAQSFAKNMGLYGERAGAFTVLCSDEE *F EAARVMSQVKILIRGLYSNPPVHGARIAAEILNNEDLRAQWLKDVKLMADRIIDVRTKLK *F DNLIKLGSSQNWDHIVNQIGMFCFTGLKPEQVQKLIKDHSVYLTNDGRVSMAGVTSKNVE *F YLAESIHKVTK *F Comments: alternatively transcribed mRNA in AT Drosophila *F melanogaster adult testes pOTB7 # *U FBrf0146844 *a Kawano *b J. *t 2002.4.21 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 22 06:36:32 2002 *F Date: Sun, 21 Apr 2002 22:36:24 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: jkawano@post.miyazaki-med.ac.jp *F Subject: FlyBase error report for CG7190 on Sun Apr 21 22:36:24 2002 *F Error report from Jun-ichi Kawano (jkawano@post.miyazaki-med.ac.jp) *F Gene or accession: CG7190 *F Release: 2 *F Gene annotation error *F Genes CG7190 and CG7193 and CG7195 should be merged. *F Comments: We have recently published a paper (cited below), in which we *F strongly suggest that CG7190, CG7193, and CG7195 are actually parts of a *F single gene for an insect homologue of the vertebrate Golgi apparatus protein *F 1 (MG-160/cysteine-rich fibroblast growth factor receptor/E-selectin *F ligand-1/latent transforming growth factor-beta complex protein-1). It is *F supported by the following evidence: 1) Three and two of peptide fragments *F from a Spodoptera glycoprotein showed high sequence homologies to CG7190 and *F CG7193, respectively. 2) It was strongly suggested that the Spodoptera *F glycoprotein was a membrane-bound protein. Although CG7190 and CG7193 could *F not encode any membrane-spanning domains, but CG7195 could do one. 3) *F Furthermore, 5' and 3' ESTs of LD19434 correspond to a part of CG7190 and a *F region downstream from CG7195, respectively. 4) The possible single gene *F containing these three genes could encode a polypeptide highly homologous to *F the vertebrate GLG1 proteins. *F Jun-ichi Kawano, Tatsuo Nakayama, Tomio Kotani, Hiroshi Matsubayashi, *F Masa-Toshi Yamamoto, Tatsuo Suganuma: Identification and characterization of *F an insect homologue of the vertebrate Golgi apparatus protein 1 *F (MG-160/cysteine-rich fibroblast growth factor receptor/E-selectin *F ligand-1/latent transforming growth factor-beata complex protein-1) with a *F Golgi-specific monoclonal antibody. Histochem Cell Biol, DOI *F 10.1007/s00418-002-0402-6. URL: http://dx.doi.org/10.1007/s00418-002-0402-6 # *U FBrf0146845 *a Gisselbrecht *b S. *t 2002.4.22 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Mon Apr 22 23:13:07 2002 *F Date: Mon, 22 Apr 2002 15:13:04 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: steveg@alum.mit.edu *F Subject: FlyBase error report for CG7821 on Mon Apr 22 15:13:04 2002 *F Error report from Stephen Gisselbrecht (steveg@alum.mit.edu) *F Gene or accession: CG7821 *F Release: 2 *F Gene annotation error *F Genes CG7821 and CG6350 should be merged. *F Gene cDNA sequence: *F CGGATATCGATACCATATCACAAACTAATAATAAAAATAATAATAAATAAGAGAATTAATACTACTAGAACACAAACAA *F AAGCATATAAAGCTTACACATCCTTTATTAAAACAAACAGCCAAATTGGATTTAAATTGTGGAACGAAGCAAACAGAAC *F CGCTTGAGTTTGTGCCACTGCCACGCAAAAAAAATTATCGTTTCCAAAAAACTAAGCATACTTCATCCGAAATTAGCAA *F TCAGCTAGCCAAAAGCGGAGTGATCTCGTCGAGAAGAGCCACGCCCCCTATCCGCCCACGCTGCCCAGAAGAGAGCAGC *F GAAGATGTCGTGGCTGAACAGCAGTCTCAGCCAGCTGAAGGGCCAACTAACCAATCTGGCGCAGGAAGTTCTGGCCGAA *F ACGGCAGGACCCGGCGACTTGGAGTATGAGGGACATCAAGCAGGAGGTGCTAGCGTCCAAGACGCCGAGCAGCAGGCCA *F AGACAGCCCTGGAACTGCTGGCCGAAACCCAGGAGCAGAAGGAGCAGCTGGACAAGCGGTGCGAGGAGAAGGATCGTGA *F GATAGCCGCCCTGCGCCGAGAACTGGCCAAAAGCAAGCAGAAACAGGAATCCCAGCTGGCAGCCAGCACATCAGCTACG *F CGGGAACCGCAGTTGCAGAATGAAGAGCCCAATGTGGAGGACAGCTGGTGCTGGGAGCCCGATGGAGGTGACGAGAAGG *F GCGCCACAGGCGCCGGATCGGGCGATTCGGCGAGCAGAGACAAGGAGTCCGGACTCGTGGACATCGCACTGGGCAACGA *F CGATGTGGTGCGGCTGAACAATCGCATCGCAGAGCTGGAGCAGCTCAATGAACAGCTAAATGTCTCGCTGGAGGAGCTG *F GACTCGCAGCATGAGCTGGCCATGCGGGATGTGCTCGAGCACAAGACGCAGCTGGCTGGTCAGGTGGCGAGTCTCAAAC *F AATTACAGGCGGATCGCCTGGTGGAGCACGAATTGTCCAATGC! *F CAGGCAGCAAAAGCAGTTGGACGAACTGCGGCAGACGTCTTCAGCCGCCAAGAAGCAGCAAGAGGAGCTGCAGCGAAGG *F GTGGAGCAGCAGGAGGCTGAGCTGATCGAGATGCAAGATCTGCTGGACAAACGTCGCCAGGACACTGCCGAGCTCATCG *F AACGCGTACGCGTGGCGGAAACGGAGCGCGAACGGCTGCTCAAGGATCTGGAGGAGACTCGGCAAGCCAAGGAGAAGAA *F GACCTCGGAGTCGTCATCGAACAGCTCCTCCACGGGCAAGCACAGCGAGGATGAGTTCATAGTTGTACGCCAAGCGGAT *F GCCACGGGCTCTGGCTCTGCTTCCGGGTCAGATCGTGATCCAGATGCCGACGTCACTTCGCCGCCCTCGAAGGAGAAGC *F TGCGCGATCGTCTCGTCTCCCTAGAATCACAAATCTCCGAGCTGACCCTCGCCAATACCCAACTGCAGGATGCTCAGCT *F GGAAAAGCAGCTGAGCATCAATATGCTGGGTGAACAGCTCGTCGAATTGGAGAAGCGCTTGCGCCTCAGCGAGGCGGAA *F AAGGAGCAGCTTCAGGTGAACTTACAGCTTCGCCTGCAGCAACTGACCGTGCAGAATCAGGAGCTGAAACTCCACGCGG *F AGGCTGAGCAGGAAGGTCACGCCCAGAATCTGGAGGAGCAGCTGGGCGATCTGCGCGAGGATAACCAACGTCTGCGTCA *F GGAGCTAAAGACCAGCATAGCCCAGGCCAAGTTCCGACAGGCCATTGCCGAGGAGAAACAGGAGATTACGGATCTGGAC *F GATGCGGACTCGGAGTACGGCACTTTCGAGCTGGATAAGCTGCGTGCTCTGCTCCAGGCCGAGATAGAGGATCGTTTGG *F ACAGCTCGTTTCCGCAGCAGAAGCTGGAGCGAGCCTGGAACGCATTGAAGGATCGCTGGCACCGCCTCGACCTGGTCGA *F GCAGCGACTGGTGGACGTACAGAACCAACAGTTGGTCAGCGAG! *F CACGAGAAGAAGACCCTCGAGGCGGACATCTCGCAGTATATTCTTCAGTGCGATGAGCT! *F GATGAAGAACAATGACCTGCTGCTCAACGAACTGGACAAATACAAGCGCAACAAGCTGGAAACCATTGAGGAGCATCAC *F GAGGAGACGATTGTGCAGCTGGAGGCCCAGTTGGAGGAGGCCAGACAGAAATTGGAATTGGCTTCTCTGTCTAGCCAAC *F AGCAGATGGAAACCCATCTGATATCCAGTCCAGAAAAAACTCCCGTAGATAGCGAGCTTCTGGCCAAAATGGAGCAAAA *F GGAGCAGGAATACTTACAATTGCAGGAGCAGTTGGCCTTCGCCAAAACGGAACTGGACAAAAGAAATAAACTGCTGGAG *F AGGAATGGGGAACAGCTGACTAAACAGCAGCAGCAAAATCAGGCAGATCAAAAGAAATTGGAAGAACTTTCACAACTGC *F GGGAGACATTGCAACGTAGGGATGAGGACCTAAAGGAGCTGGAAGAGCAATTAAGCGCCGTTAGACAGGATCTAGATGA *F GAAGTCCATTCAGATGAAGATCAGTCAGGATCAGCACAAACTCCAGTTGGCCAATCTCCAAAATCAGTTGCAAGCCGAT *F CAGGAGAAGCTAAGAGAACTGCTCCAGCTGCAGGACAAACTGGAGCAGCAAAAGGAGCTCATGGAAGTCGACCAGAATC *F AACAGATTACCATAATCAAAAAGGAGCTGGCCGAGACGACCAATCAATTGAGTGAGTGCCAGGAGAGGCTAACCGTCAA *F GGAAGCCCAGCTGGCGGAGATCCAACAGCAGTTGCAAGAAGTTAACGAGGAAAGAACAAGGTTGCAGGAGCAACTCCTG *F ACCAAGGAGCAAGAATCTGGCTTGGACTCCGAGCTGGCAAAACGGAATCAGGAGCTTGAGGATCAGCTGCTAGCCAAGG *F AGCAGCAACTTCAGCTAAACCAAGCTGAACTGGAAAAACTACAAGAGACACTTCGAGTTAACGAAGAGCAGCTCCTTGC *F CAAGGAGGAGCAGCTTCATGCCAAGGAATCCCAACTGCAGTCT! *F CTGGAATCACAATTGCAGGGTCAGCTAGCTGCCGATGAGAGCCAGCAACTTCAGCAGACCATCGATGGCTTGGGACAGG *F AGAAGAATGAGCTGATCAAGGTACTGCAGCAAAAGCACCAGGAGAACACCCAGTACTATGCGGAAATCCAGCGACTGCA *F GCCCTTCGAGCAGCAGGTAAAGGAATTGGTCAAGGAGCGCGAGAAGCTGCAGGATCAGGTCGGCTTTCTCAAGGAGAAG *F TCCGACATACTCACCACGAATCTGCTGACGGAACAGACCAACCAGCGACTCCTGCAGCAACAGCAGGCGGAGTCCCAGG *F AGCAGCAGGCCAGCACTTTGCGGGATCTGGAACGTTTGCGGGCCCATCTCCTTGAGATCGAGGAGCTGCACACGCAGGA *F AACTGTCGAGCTGCAGCGCGATCTGGAGGAGTCGCGCTCCCGTCAAGCGATCCTCGAGCAGCAGGTTTCCAAATCCAGT *F ACGGCGTACACATCGGCCAGCATCCGTGCGAATCAGCAGGCGGAGACGCTCCAGGCGCAGCACGCTCTGCTCCAGCAGC *F AGCGTGATGAGCTATTGGCCAAACTGGGCCAGTACGAGGATCGGGAGCTGAAGCAGCAGGCGGCGCTGACCAATCTGCA *F GTGTGCCCTGGAGCAGTTCCAAAATGACAAGGATCATGACATCGAAATGGCCACGCAGAGGATTCGCCGCGAGATGCAG *F GCCCAGTTGGACCGACAGGGTCAGCTCCAGCTGGAGATGAGCGGCCTGCAGCAGCAGCTGGCGGAGGCCAATCAAGGAT *F TGCGAGCTGCAGCCCGACTCTCGGATCAACTGGAGGCTGGACAGCAGACGATCGCCGTGTTGCGTGATGAAGTGGAGAG *F CCTAAAGGAGGCGAATGGCCAGCTGGAGCAGCGGCTCAGCAGCAGCGAGAGCAGCCAGACGGACAAGATTGACAAGAGC *F TTGATCAAGAGCCTGCTCATTGGCTATGTGGTCAGTGGTCATG! *F CGGGGGATAAGCAGCAGGTGCTGCGCATGATCTCCTCTGTGCTGGACTTTAATGCCCAG! *F GAGGCGGATAAGGTGGGACTCAATAAGCAGCAAAGCAGCTGGCTGGGCGCCATTCTCGGTGGCGGTTCCGCCACAGCGT *F CGGCAGCAGGATCTTCGCGTGGCAATGAGAACCTGGTGCAGGCCTTCGTTCAATTCCTGGAGCAGGAGTCGCAGCCGCA *F GGCGCAATCGCGACCCACGTTGCTGAGCATGACGGGCCAGATGGATGGAGTGACATCCGCCAGTGCCACCACTTCCACA *F GCTGCCACGTCAGGCCATCTTCCGCAGGCTTCTTCTTCATTTTCACCGGCGACACCAGCAGCAAGTCAGCAGGATCCTG *F CGGCTCCTCGCACACCGCCCGTGGGTGGCTCACCACAGCTGCTGGCCATGGGCTCCAACGAATTTGCGCCCACACGCAA *F CTCCAGCTCGATATTGAAGGACATCCTCAGCGACTCCTGATTGTTGTAGAATAGTCGCGTCTTTATGTTGTCATGTGTT *F TTTTTTATTGGATAATTTTAGCATATTTTAAACCCACTTGTAAACGTGCAATCGTGTCGCCCGTATTCGGAAACATATG *F TCCCGCATATATCTCGCTATATATATATATATATTTAACTATATATATCTCCAAAACTGTAAAATGTCTGTATGTGTAT *F ATGTGCAACACTAGCAACTAATATGCAACTTGAACATGTCGAGGGCAGCAGAAAATAGTAGAACCCAATGGTTTTGGCC *F AGTTTCGCTTGCCTGTGACACGCCTTCACAGCGGCCTTGCTAATGAGTAGCCGCCAAATAATAAAAATAATTAAATAAC *F TAAACGAAAAAAAAAAAAAAAAAA *F Protein sequence: *F MSWLNSSLSQLKGQLTNLAQEVLAETAGPGDLEYEGHQAGGASVQDAEQQAKTALELLAETQEQKEQLDKRCEEKDREI *F AALRRELAKSKQKQESQLAASTSATREPQLQNEEPNVEDSWCWEPDGGDEKGATGAGSGDSASRDKESGLVDIALGNDD *F VVRLNNRIAELEQLNEQLNVSLEELDSQHELAMRDVLEHKTQLAGQVASLKQLQADRLVEHELSNARQQKQLDELRQTS *F SAAKKQQEELQRRVEQQEAELIEMQDLLDKRRQDTAELIERVRVAETERERLLKDLEETRQAKEKKTSESSSNSSSTGK *F HSEDEFIVVRQADATGSGSASGSDRDPDADVTSPPSKEKLRDRLVSLESQISELTLANTQLQDAQLEKQLSINMLGEQL *F VELEKRLRLSEAEKEQLQVNLQLRLQQLTVQNQELKLHAEAEQEGHAQNLEEQLGDLREDNQRLRQELKTSIAQAKFRQ *F AIAEEKQEITDLDDADSEYGTFELDKLRALLQAEIEDRLDSSFPQQKLERAWNALKDRWHRLDLVEQRLVDVQNQQLVS *F EHEKKTLEADISQYILQCDELMKNNDLLLNELDKYKRNKLETIEEHHEETIVQLEAQLEEARQKLELASLSSQQQMETH *F LISSPEKTPVDSELLAKMEQKEQEYLQLQEQLAFAKTELDKRNKLLERNGEQLTKQQQQNQADQKKLEELSQLRETLQR *F RDEDLKELEEQLSAVRQDLDEKSIQMKISQDQHKLQLANLQNQLQADQEKLRELLQLQDKLEQQKELMEVDQNQQITII *F KKELAETTNQLSECQERLTVKEAQLAEIQQQLQEVNEERTRLQEQLLTKEQESGLDSELAKRNQELEDQLLAKEQQLQL *F NQAELEKLQETLRVNEEQLLAKEEQLHAKESQLQSLESQLQGQLAADESQQLQQTIDGLGQEKNELIKVLQQKHQENTQ *F YYAEIQRLQPFEQQVKELVKEREKLQDQVGFLKEKSDILTTNL! *F LTEQTNQRLLQQQQAESQEQQASTLRDLERLRAHLLEIEELHTQETVELQRDLEESRSRQAILEQQVSKSSTAYTSASI *F RANQQAETLQAQHALLQQQRDELLAKLGQYEDRELKQQAALTNLQCALEQFQNDKDHDIEMATQRIRREMQAQLDRQGQ *F LQLEMSGLQQQLAEANQGLRAAARLSDQLEAGQQTIAVLRDEVESLKEANGQLEQRLSSSESSQTDKIDKSLIKSLLIG *F YVVSGHAGDKQQVLRMISSVLDFNAQEADKVGLNKQQSSWLGAILGGGSATASAAGSSRGNENLVQAFVQFLEQESQPQ *F AQSRPTLLSMTGQMDGVTSASATTSTAATSGHLPQASSSFSPATPAASQQDPAAPRTPPVGGSPQLLAMGSNEFAPTRN *F SSSILKDILSDS *F Comments: These two computed genes are both present in the BDGP cDNA sequence *F SD07366.complete. I have personally confirmed that they are part of a single *F message by RT-PCR. # *U FBrf0146846 *a Collett *b J. *t 2002.4.9 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 09 20:25:54 2002 *F Date: Tue, 9 Apr 2002 12:25:49 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: jcollett@oeb.harvard.edu *F Subject: FlyBase error report for Dip-B (FBgn 0000454) on Tue Apr 9 12:25:48 *F 2002 *F Error report from Janet Collett (jcollett@oeb.harvard.edu) *F Gene or accession: Dip-B (FBgn 0000454) *F Release: 2 *F Gene annotation error *F Gene CG 9285 corresponds to FBgn0000454 *F Comments: CG9285 identified in the annotation of 87F13 is the gene Dip-B *F previously mapped to the region 87F12-15 (Jarman and Collett, 1998). # *U FBrf0146847 *a Collett *b J. *t 2002.4.9 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Tue Apr 09 20:31:36 2002 *F Date: Tue, 9 Apr 2002 12:31:35 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: collett@oeb.harvard.edu *F Subject: FlyBase error report for Dip-C (FBgn 0000455) on Tue Apr 9 12:31:35 *F 2002 *F Error report from Janet Collett (collett@oeb.harvard.edu) *F Gene or accession: Dip-C (FBgn 0000455) *F Release: 2 *F Gene annotation error *F Gene CG5663 corresponds to FBgn 0000455 *F Comments: CG5663 identified in the annotation of 87B15 as an Xaa-Pro *F dipeptidase is the *F gene Dip-C (Fbgn 0000455) previously mapped to the left of Pp1 as in the *F region 87B5-7. # *U FBrf0146848 *a Ray *b K. *t 2002.4.27 *T personal communication to FlyBase *u *F >From rd120@gen.cam.ac.uk Mon Apr 22 15:32:08 2002 *F To: krishanu@tifrc3.tifr.res.in *F Subject: Helping FlyBase: ADRC-1008C *F Dear Dr. Ray, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Investigating the role of KAP (Kinesin Associated Protein) in Drosophila'. *F You mention a gene that is new to FlyBase, Kap. Do you know which of *F the Genome Project CG annotations your gene corresponds to? Is it *F CG11759 by any chance? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. If your gene does not *F correspond to a CG then perhaps you could tell me its map location, as *F this is valuable information for the genome annotation project. *F Thank you very much for your help. *F Best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F >From krishanu@tifr.res.in Sat Apr 27 06:00:56 2002 *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: RE: Helping FlyBase: ADRC-1008C *F Date: Sat, 27 Apr 2002 10:32:16 \+0530 *F Dear Rachel, *F ... *F The BDGP gene annotation no. CG11759, predicted ORF CT4241, Flybase acc. no. *F Fban 0011759, we found that it is homologous to the SpKAP115 of sea urchin, *F KAP3A and KAP3B of mouse. We have also identified EMS and P-insertion lethal *F mutations in the gene which maps close to the sisA locus. We labelled the *F gene DmKAP and a manuscript describing the mRNA expression pattern has ben *F submitted for publication recently. *F sincerely, *F krishanu. # *U FBrf0146849 *a O'Farrell *b P. *t 2002.4.27 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Sat Apr 27 15:38:06 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail/redundant listing of a gene/Grunge/Atrophin *F comments: Flybase lists both Atrophin and Grunge as genes. These are the *F same gene which was described in two different recent reports \- one from the *F Xu laboratory in January in Cell (they called it Atrophin) and one from *F Kerridge laboratory in March in Development (they called it Grunge). *F mailto: flybase-help@morgan.harvard.edu *F realname: Pat O'Farrell *F reply-to: ofarrell@cgl.ucsf.edu *F Sent from computer adsl-63-203-70-200.dsl.snfc21.pacbell.net # *U FBrf0146850 *a Christensen *b T. *c G. *d Karpen *t 2002.4.22 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Apr 22 15:32:56 2002 *F To: Calvi@mail.med.upenn.edu *F Subject: Helping FlyBase: ADRC-1012A *F Dear Brian, *F Please feel free to pass this on to first author T. Christensen \- who is *F not in the FlyBase people list so I couldn't address it to them myself. *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'DmMcm10 interacts with members of the Pre-RC'. *F You mention a gene that is new to FlyBase, Mcm10. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. If your gene does not correspond to a CG then perhaps *F you could tell me its map location, as this is valuable information for *F the genome annotation project. *F Thank you very much for your help. *F Best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From twc4@cornell.edu Mon Apr 22 17:38:52 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Mcm10 *F Rachel, *F Thanks for your email, it got to me through Brian Calvi. Mcm10 *F corresponds to CG9241. Although Flybase reports the incorrect AA length. *F I've determine experimentally that the protein is 776AA with a MW of 86.5 kD. *F I also have a question for you as to naming. Gary Karpen's group identified *F Scim19 which is a P element insertion 76bp 5 prime of the start codon for *F CG9241, although it has not ben shown that the P element affects CG9241 or *F CG9242(bur). Thanks--Tim # *U FBrf0146853 *a Mlodzik *b M. *t 2002.5.6 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon May 06 16:24:31 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{arm-lacZ.V} insertions *F The following information was provided to the Bloomington Stock Center by *F Marek Mlodzik, Mount Sinai School of Medicine (5/02). *F P{arm-lacZ.V}51D is a homozygous viable and fertile insertion located at *F 51D1-2. *F P{arm-lacZ.V}36BC is located at 36BC. *F P{arm-lacZ.V}83B is located at 83B5-8. *F All three insertions were isolated and mapped by Marek Mlodzik and colleagues. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0146854 *a Caggese *b C. *t 2002.4.27 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Sat Apr 27 11:33:16 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Sat, 27 Apr 2002 11:33:16 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sat, 27 Apr 2002 03:33:10 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG3861 on Sat Apr 27 03:33:10 2002 *F X-Virus-Scanned: by AMaViS perl-11 *F Content-Length: 2935 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG3861 *F Release: 2 *F cDNA or EST error *F Gene cDNA sequence: *F >CG3861 alt 1|mRNA *F AATAAATTTTTGTATCCTACATCACTAGCTTTCACATTCCCAAAAACAAGAGAAAAAGCG *F AACTAGAAACTTCGAAATATCGTATATATTTACAGCTAAAAGTAGTAATGTTCGTACGCC *F GTTTCGGAAATGGCTCTTTGGGCCATCATTTTGCCTTTCAACGACGTTATTTGGCTAAAA *F ATTCTATGTGGGACTCGAAGTCCATTGGGACTCCAAGTTCATTCCGAATGCGTTCCAAAA *F AGGATAACGTGACTGGCAATCTCAAGGAGCAGCAACAATCGGCGACTCCGGAAACCTTGA *F ACATGCCTGATATGCAGGATGCCCAAAAACTGCCAAATGTGGGAGCCTATGTTCGCATGA *F TCGCCGCCGATGGCAAGAGCCTGCGCGACGTGCTGGCCGCCAAGGTGCCCCAGGAGCAGG *F AGCGCGTAAAGAACTTCCGCAAGCAGCATGGCGCCACCAAGATGGGCGAGACGACCATCG *F ACATGATGTACGGCGGCATGCGCGGCATCAAGGCCCTGGTCACCGAGACCTCGGTGCTGG *F ATGCTGACGAGGGTATCCGCTTCCGCGGCCTCTCCATTCCCGAGTGCCAAAAGGTTCTGC *F CAGCCGCCGATGGCGGCACTGAGCCCCTGCCCGAGGGTCTCTTCTGGCTGCTGTTGACCG *F GCGAGGTGCCCACCAAGTCCCAGGTGCAGCAGCTGTCCCGCGAATGGGCCGAGCGCGCCG *F CCCTGCCCCAGCACGTGGTCACCATGTTGAACAACATGCCCACCACCCTGCACCCGATGT *F CGCAGTTTGCTGCCGCCGTCACTGCGCTGAATCACGACAGCAAATTCGCCAAGGCATACT *F CGGATGGTGTGCACAAGAGCAAGTACTGGGAGTACGTCTACGAGGACAGCATGGACCTGA *F TCGCCAAGCTCCCAGTGGTGGCGGCCACCATCTACTGCAACACCTATCGCGGCGGCAAGG *F GCTCCCGTTCGATTGACTCCAGCCTGGATTGGTCGGCGAACTTTGTGAAGATGCTCGGCT *F ACGACAACGCCCCCTTCACCGAGCTGATGCGTCTCTATCTGACCATCCACAGTGACCACG *F AGGGTGGCAACGTGTCTGCCCACACCGTTCACTTGGTGGGCTCCGCCCTCAGCGATCCCT *F ACCTCTCCTTTGCCGCCGGCCTGAACGGTCTGGCTGGTCCCCTGCACGGCCTGGCCAACC *F AGGAGGTGCTCGTGTGGCTGCGCAAGCTGCAGAAGGAGGCCGGCAACAACCCGTCGGAGG *F AGCAGCTCAAGGAGTACATCTGGAAGACCCTCAAGTCCGGACAGGTGGTTCCCGGCTACG *F GACACGCCGTGCTCCGCAAGACCGATCCCCGCTACACCTGCCAGCGTGAGTTCGCGCTGA *F AGCACCTGCCCGAGGACGAGACGTTCCAGCTGGTGTCGAAGATCTACAAGGTGGTGCCAC *F CAATTCTGACCGAGACCGGCAAGGTGAAGAACCCCTGGCCCAATGTAGATGCCCATTCCG *F GTGTGCTGCTGCAGTACTACGGCATGAAGGAGATGAACTACTACACCGTGTTGTTCGGCG *F TTTCCCGTGCCCTCGGCGTGCTGGCCTCCCTCGTCTGGGATCGCGCCCTCGGCCTGCCCA *F TCGAGCGCCCCAAGTCATTCTCCACCGATCTGCTGGTCAAAATGGTCCAGAAGTAAGCAA *F CTGGAGCAGGAGGAGCGGGAGGAGCTGGAGTTCAACCTTTTGTAGCCGGAGGACGATGGA *F CGTAAAATGCAGAAGCTGTAGATTGTAATTGACGAGGGAAGAGGCAGAAAGAGAGAGATA *F GAGAGAGAGAGAGAATCGATCGGATTGGATTAGGATGAGATGGGATTGGATTGGATGGAT *F ATCGATATGACGACGCTGATATTGAGGGAGTTGGAGATGTGTAGTTGAGGAGAAGAGCAA *F CCAAATACGAACCAATAGGAC *F Protein sequence: *F >CG3861 alt 1|CDS *F MFVRRFGNGSLGHHFAFQRRYLAKNSMWDSKSIGTPSSFRMRSKKDNVTGNLKEQQQSAT *F PETLNMPDMQDAQKLPNVGAYVRMIAADGKSLRDVLAAKVPQEQERVKNFRKQHGATKMG *F ETTIDMMYGGMRGIKALVTETSVLDADEGIRFRGLSIPECQKVLPAADGGTEPLPEGLFW *F LLLTGEVPTKSQVQQLSREWAERAALPQHVVTMLNNMPTTLHPMSQFAAAVTALNHDSKF *F AKAYSDGVHKSKYWEYVYEDSMDLIAKLPVVAATIYCNTYRGGKGSRSIDSSLDWSANFV *F KMLGYDNAPFTELMRLYLTIHSDHEGGNVSAHTVHLVGSALSDPYLSFAAGLNGLAGPLH *F GLANQEVLVWLRKLQKEAGNNPSEEQLKEYIWKTLKSGQVVPGYGHAVLRKTDPRYTCQR *F EFALKHLPEDETFQLVSKIYKVVPPILTETGKVKNPWPNVDAHSGVLLQYYGMKEMNYYT *F VLFGVSRALGVLASLVWDRALGLPIERPKSFSTDLLVKMVQK *F Comments: AT12538.complete AY089318 identifies an alternative transcrip for *F this gene *F Browser: Mozilla/5.0 (Macintosh; U; PPC; en-US; rv:0.9.4) Gecko/20011022 *F Netscape6/6.2 *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0146855 *a Hortsch *b M. *t 2002.5.1 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Wed May 01 16:32:06 2002 *F To: flybase-updates@morgan.harvard.edu *F Cc: hortsch@umich.edu *F Subject: FlyBase error report for CG7462 on Wed May 1 08:32:04 2002 *F Error report from Michael Hortsch (hortsch@umich.edu) *F Gene or accession: CG7462 *F Release: 2 *F Missed gene *F Gene cDNA sequence: *F >ANTTTTGGAGTTGAAACATNGGAAGGAAAACGGGCAGCAAAACNGAAAAATTGTTCCTTCGAGAACCGTGAACTTAAT *F AAGGGACTGTGTGAGAGCAGCCAGGAGTCCAATCAGACACATACACACACACAAAGACATTTACACCCATACACTGACA *F CTGACACGCACACAACCGAATCGGTCGCCTGTACGAACACACCCTCTCACACACTCATGCACTCGTGTACATATAGCTT *F ACATAAAGTCTAAGGAAAAATCGATAGGAGTGCATTAATAATTCAGCGAAAACGAAACGAGCATAAGGTCTCGAGCAAA *F AATCTTCAAGTAGTGCGAGTTTTCCGCCTCTGCTAACGGTGAAGAAAAGTTCCAGGCCTCGTGAAAAAATATGACTAAT *F CTGCAGTGACGACATACGCGCCGCTTACACAATTTCTCTACACCAATTAACCGCTGAAATCAATTCAAGACGGGCATTT *F TACCATTGGCAATTGTGAAAAAATGGTCACCGAAAATGGAGCACAGGGCGATGGAAACACTTCCTTCCTGCGTGCCGCT *F CGCGCCGGAAATCTGGAGCGAGTGCTCGAGCATTTGAAGAACAACATTGACATTAACACCAGCAATGCGAATGGCTTGA *F ATGCCCTGCACCTGGCCTCCAAGGATGGCCACATCCACGTCGTCTCGGAACTCCTTCGTAGGGGCGCGATTGTGGATAG *F TGCCACCAAAAAGGGAAACACTGCCCTCCACATCGCCTCATTGGCTGGACAGGAGGAGGTGGTGAAGCTACTGCTGGAG *F CACAATGCCTCCGTGAATGTGCAATCCCAGAATGGATTCACTCCACTCTACATGGCTGCCCAGGAGAACCACGATGCTG *F TGGTGCGTCTCCTTTTGTCCAACGGAGCCAACCAGAGTCTGGCCACCGAGGATGGCTTCACCCCACTGGCGGTGGCCAT *F GCAGCAGGGTCATGACAAAGTCGTTGCTGTCCTCCTCGAGAGT! *F GATACTCGTGGAAAGGTCAGGCTTCCTGCGCTGCACATTGCCGCCAAAAAGGACGACGTCAAGGCAGCGACTCTGCTTC *F TCGATAATGACCATAATCCGGACGTGACTTCCAAGTCGGGCTTCACCCCGCTGCACATTGCTTCCCATTATGGTAACCA *F AAACATCGCCAACCTACTCATCCAGAAGGGCGCCGATGTCAACTACTCGGCCAAGCACAACATCAGTCCGCTGCATGTG *F GCTGCTAAGTGGGGTAAGACCAACATGGTTTCCCTGCTTTTGGAAAAAGGTGGTAACATCGAGGCAAAGACCCGGGACG *F GACTTACTCCGCTCCATTGCGCCGCTCGCTCGGGTCATGAGCAAGTTGTTGACATGCTACTCGAACGAGGAGCTCCCAT *F CTCTGCCAAGACCAAAAATGGTTTGGCACCCCTCCATATGGCCGCCCAGGGTGAGCATGTTGATGCCGCCCGCATCCTC *F TTGTACCATCGTGCTCCCGTCGACGAGGTGACAGTGGATTATCTCACTGCTCTTCATGTGGCTGCTCACTGTGGTCATG *F TTAGGGTGGCTAAGCTTTTACTAGATCGGAATGCTGATGCCAATGCAAGGGCTTTGAATGGATTCACTCCACTGCACAT *F TGCCTGCAAGAAGAACCGCTTGAAGGTGGTAGAGTTGCTCTTGCGACATGGTGCCAGCATTAGTGCCACCACAGAGAGT *F GGACTTACTCCGCTCCATGTGGCCGCCTTTATGGGCTGCATGAACATTGTCATCTATCTGCTGCAGCACGATGCCAGTC *F CCGATGTGCCCACTGTGCGCGGAGAAACGCCACTCCATTTGGCCGCCAGAGCCAACCAGACCGACATCATTCGCATCCT *F GCTGCGCAATGGCGCCCAGGTGGACGCTCGTGCCCGAGAGCAACAGACACCGCTGCACATCGCTTCGCGACTGGGTAAT *F GTAGACATCGTGATGCTTCTGCTGCAACATGGCGCCCAAGTGG! *F ACGCAACCACCAAGGACATGTACACGGCACTTCACATCGCTGCCAAGGAGGGCCAGGAT! *F GAGGTGGCCGCTGTACTAATTGAGAACGGCGCCGCCTTGGATGCCGCCACCAAGAAGGGATTCACTCCACTTCACTTGA *F CGGCCAAATATGGACACATTAAGGTTGCCCAGTTGCTGCTCCAAAAGGAGGCAGATGTGGATGCGCAGGGCAAGAACGG *F TGTCACCCCGTTGCATGTGGCTTGCCACTACAATAACCAGCAGGTTGCCTTGTTGCTTTTGGAGAAGGGAGCAAGTCCA *F CATGCCACGGCCAAGAATGGACATACCCCGCTCCATATTGCCGCAAGGAAGAACCAGATGGACATAGCTACTACTCTAT *F TGGAATATGGCGCTTTGGCCAATGCCGAGAGCAAAGCCGGATTTACTCCACTCCATTTGAGCAGCCAGGAGGGTCATGC *F CGAGATCTCCAATCTGTTGATCGAGCACAAGGCCGCCGTCAATCATCCGGCTAAGAATGGTCTAACTCCCATGCATCTG *F TGTGCCCAGGAGGATAATGTGAATGTGGCTGAGATTCTGGAGAAGAACGGCGCCAATATCGACATGGCCACCAAGGCAG *F GCTACACTCCCTTGCATGTGGCCTCTCACTTTGGACAGGCCAACATGGTGCGTTTCTTGCTGCAGAATGGCGCCAATGT *F GGATGCGGCCACCTCAATTGGCTATACTCCGCTCCATCAGACCGCCCAGCAGGGTCATTGTCATATTGTGAATCTCCTG *F CTGGAGCACAAGGCTAATGCCAACGCTCAGACGGTCAATGGACAGACGCCTTTGCACATTGCCCGCAAACTGGGCTACA *F TAAGTGTCCTAGACTCGTTGAAAACCATCACCAAGGAGGACGAAACAGCTGCTGCTCCTTCCCAAGCCGAGGAGAAGTA *F TCGCGTGGTGGCTCCCGAGGCCATGCACGAATCCTTCATGTCCGACTCCGAGGAAGAGGGCGGCGAAGACAATATGCTA *F TCAGATCAACCATACCGCTATTTGACCGTTGATGAAATGAAAT! *F CCCTAGGCGACGACTCGCTGCCCATCGATGTGACCCGTGATGAGCGCATGGACTCCAACCGGATGACCCAGAGCGCCGA *F GTATGCCTCTGGTGTTCCGCCCACCATTGGGGAGGAGGTGATCAGTCCCCACAAGACCCAAGTCTACGGCAGTTCACCC *F AAGGCCACCGTGGATGGAGTGTACATTGCCAATGGATCTGGTCACGATGAGCCGCCGCATGTGGGTCGCAAGTTAAGCT *F GGAAGAGCTTCTTGGTCTCCTTCCTGGTGGATGCCCGTGGAGGAGCAATGCGCGGCTGTCGGCACAGTGGCGTTCGCAT *F GATCATCCCTTCGAGGTCCACTTGTCAGCCAACCAGGGTAACCTGTCGTTATGTAAAGCCGCAGCGCACAATGCATCCG *F CCCCAGTTGATGGAGGGCGAGGCTTTGGCCAGTCGTGTCCTGGAACTGGGTCCTTGCTCCACCAAGTTTATTGGGCCAG *F TGGTCATGGAGGTGCCACACTTTGCATCGCTGCGTGGCAAGGAGCGCGAGATCATAATATTGCGATCGGACAATGGAGA *F AACATGGCGGGAGCACACCATCGATAACTCCGAGGAGATCATACACGATGTCCTGCAGCAGTGCTTTGAACCAGAGGAG *F ATTGCCCAACTGGAGGAGCAGGCTGGCAACCATGTCTGCCGCTTTGTCACCTACGACTTCCCTCAGTACTTTGCCGTCG *F TATCGCGCATACGCCAGGAGGTCCATGCCATTGGACCGGAGGGCGGCATGGTGTCAAGCACCGTGGTGCCACAGGTGCA *F GGCCGTCTTCCCACAGGGAGCCCTCACCAAGAAGATCAAAGTTGGCTTACAGGCCCAACCGGTTGATCCCGATCTCACG *F GCGAAGCTTTTGGGTCGCGGCGTGGCCGTATCCCCAATTGTTACGGTCGAGCCGCGTCGCCGTAAATTCCACAAGGCCA *F TCACCCTCAGCATGCCCGCCCCCAAGGCTCACGGCCAGGGTAT! *F GATTAACCAGTACTCGGGCAATACGCCCACCCTGCGTCTGCTATGCTCCATTACAGGCG! *F GACCATCTCGTGCTCAATGGGAAGATGTCACCGGTTCCACGCCCTTGACATTTGTCAATGACTGCGTCTCCTTTACGAC *F CACAGTATCAGCTCGTTTCTGGCTGATGGATTGCCGCAACATTTCGGATGCCACCAAAATGGCAACTGAACTGTACAAG *F GAAGTCATCCATGTGCCGTTTATTGCCAAGTTTGTGGTATTCGCCAAGAAGGTAGAACCATTCGAAGCAAGACTCCGAG *F TCTTTTGCATGACCGACGATCGTGAGGACAAAACTCTGGAGAAGCATGAGCTCTACACAGAAGTGGCCAAGAGCCGCGA *F TGTCGAGGTCCTCGAAGGCAAGCCGCAGTACATCGAGATGGCTGGCAATTTGGTGCCAGTGACCAAATCTGGCGACCAG *F CTCCAGGTGCAATTCAAGGCCTTCCGAGAGAACCGCCTACCGTTCACAGTACGAGTCAAGGATCAACATGCCGACATTG *F TTGGACGCACTTTGTTCATGAAGGAACCAAAGGTAGCCAAGGGCGAGCCACCGCAACAGCCCATCTGCATCCTGAACAT *F CGTTCTTCCGGAAGCTGTAATTCCCGATTCCACGACCGCCTTTTCGGACCGCGTCACCTCCGCCTACAGGACCTCAATG *F TTCAGCTTGAGCAAGCATCAAAATGACCATTACATTGGCGACATCCGCATCGTCGATCTATCCAACCTGTTGGGTAAGG *F ATTGGATTCAGCTGGCCCCGGAGATTGGCATCAATGGAGAGGAGATCGATGAGATCATCAACCAAAACACAGATAGCAT *F TGCCAGGCAGGCGCAGAGCATGATTCGCCTGTATAAGGACAAACCTAACTACGATATTCTTTCGCTGGAAACTGCTCTC *F AAAAACATTGGACGAGACGACATCATGAAAAAGTGCAAGAGTGGACGACTATCGCATTCGCGTGAATTCGATGAGGCGG *F ACCTTATGAAGAACTCGGAGTCCGTGGAGGAACTGGTTCGCAG! *F GGAAAGCAAGCGAATCCAGCAAATTAACGAACGCGAAGAAGTCAAATACTCTGCCGAGGAGAAGGAGGTCGAGGAATCG *F GAGTCTGATGAAGAAGCTGCCAAGCGAACCGTAGCCGAACGGCGGGAAAAGATTGTTAAGCGTCTGTCCATCGAACGAT *F CAATTCCAGCCTCCACTCAGAAAAAAGAGATCACCCGCGAAATAACCGAAATCAAGCGTAAGAGTCTAATCGAGGACAA *F GAAGGCTCACCATGAGTCCGAAATTCTTATGCAACTGCCTGCGGATAACGTGATTATCAAGACAACAACTGTTCCCGAC *F CAAGTCATTAAGATGAAGATGGGCAAGATGGACTCAACCGAGGTGAGCAAGAGCGAGTTCGACAAGGAACTTACGCACA *F AGTTCAAGACATCAGGGCGTAGCTCAGAGGAGGAAGACCAGCCATCGTCTCCAGATCAGACTGACAAGATTGTCCAGGA *F CATTAGTGCTGCCGAAAAGAAGGAGAAGGATGGCGTTACCTTCAGCCGAGTGACGACAATAACCCGCCAAGAGGCCCGC *F GACATTACCGAAGACTTCTTAGAGATTGAGAAGCGCAGCCAGCTCCCGGCGACTTCCACAACCGCAACAGTTCATGAGA *F AGTTCGTTGAGGAGATCAAGGAGAAGACCAGCCCTCTGGCTTCAGTGCCACAAGAAACAGTCAAGGAGGTGCAACAAGT *F CATCAGCGAGGTGACCGAAATAGCCAGCAAGAAGGTTGAAAACATTATTAGCTCCTTTGAGAGTAGCAAGTCTGTGGAT *F GCTACTACAGTTCTGCCCACACAGCCGTCTGTTGAATCAACGAAGGTTAGCGAGACCATTAAGAACCTCGAGGATGCCA *F AGGCCGTGTCTGCGGAACAAGTGAAAACTGTCCATGTCGTTGAGAGCTCTAGTATCGAGGAGACTATTGCCGAATTCGA *F AGCCAAGAAGGTTAAATATGACTTCCATGGCGGTGAGCCCAAG! *F ACACAGATCCCAAAGTTTACGCGGAAACCAAGCGATGACTCTATGAAGCCCACCGCAGC! *F TCCACGTGCTACAGTTGAATCCGAAACTGAATCGGTCATTGAGACTAAGGCCGAAAAACCGATCTCAAAGATACCAGTA *F AAGACAATACCAACAGAAGCTCAGAAGGTTAGCGAAGTAGATGCACGTAAGATTACTCAAGACTTCCTCCAGGGTGAGA *F AACTGGCGGCCGAACCCAAGCCATCGCCAGCAGCCAGCAAGATACCAAAGGTGGAGCCGAGGAAATCTGTTGATAAGCA *F GGTCGATCGGGAATCAAAAATCTTGGATGATGTTGTGGCCTCTACGGCCACCATTATGACCGCCGGACTGGGAGATGAG *F CAGCTCAAGGATCAACTCGTCGATCACTCAGAAATTATCGCCAAGTCCGAAACAGTCGCTGAAAAGGTAACCGAACTGC *F TTGACACATTCCACAAGATCGAGGAGAAGGTCACCAAATCTAAAAAGACCTCGGAAATATCGAGCAAAGTGGAGGAACT *F GGTTAAAATCGAGGAAAAACCTCTCTCACAAGTTCAACCCAAGGAGGACAAGGTGGCGGAAAAGGTTGCTGAAGTTATC *F GAAACATTCCACAAAATCGAGGAGCAGATCACCACTGCCGATGCCCGCGAGCTAACTCGTGATTTCCTGAGCACGGAAC *F AACGAAACCAATTGCCGAGCATGCCACAAGCTGCTGAGAAGCCCCTTGAATCTAGCTTGACATCCACCAGTGCCTCGGA *F ACCAGAATCAATAGTCACAGTGAAGCCATCACCCCCAGCGAGTAAGATACCTGTAGTGGAACCCAGGAAAATCGTTTTC *F GACGAATCTACAAAGCCTCTGATCGAGCCTGAGCCAGTGAAGACCACTGAAAAGAAGCCTCTTAATGAGCGACAACTAA *F CCGCTGATTTCTTGAGCATGGAGCAACAAACCCAGCTGGTCTCTGAGCCTGCCAAATCGTTGGTGGAAGAGGTGATGAA *F GTCCGCCGAACAAATGGTGGAGCAACCGAAACAGCAAAAATCC! *F CTGAATGAGCGACAACTTACAGAGGACTTTCTGCTTATGGAACAGCAGACACAACTGCATTCAGACATTGTTAAGCCAA *F CGGATAAGCTGATCGATGGTATTGAGTCCGCAGCGCCGGTTGGAGATGAAGCATCACCGTTCCACACGCCCAAGCTGAC *F CACCAGTGTGGTGACACAGGAGTCACAGCACCTGGCGAGTGAATATGACAGTGACACTTTCGGCAAACAGGCCACCATA *F CCGTTAGGAGACTCAAAGATTGATCAGGGACTCACTGCACCAGTATCCATGGAACCACGAAAGTCACTGACCGATGCCG *F AGTTCTGCAAATCGGTGGGCGAAACAATCACCAAGAAGATGAGTGTGGGAGTTATTGAAATAAGTGATGAGCTCAAGAA *F AATTGAGAGCGAAATTCCACATTCCCAGACTCCTCCGCCAACACCGAGTGACAACAAAACCGACAAGCAGAACGAAGAG *F CCAGAACTGATCAGCTTGGAACGTGATTACCTGGCCGACACTGTGACCACCCTTCACACAACTACAGAGTCGACCCTCG *F AACGCGTTTCTGCCATTACTAAAATTGGTAAGTGCAAGTCCTGA *F Protein sequence: *F >MVTENGAQGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGN *F TALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDK *F VVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK *F HNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGE *F HVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGA *F SISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPL *F HIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQ *F LLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAES *F KAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHF *F GQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTIT *F KEDETAAAPSQAEEKYRVVAPEAMHESFMSDSEEEGGEDNMLSDQPYRYLTVDEMKSLGDDSLPIDVTRDERMDSNRMT *F QSAEYASGVPPTIGEEVISPHKTQVYGSSPKATVDGVYIANGSGHDEPPHVGRKLSWKSFLVSFLVDARGGAMRGCRHS *F GVRMIIPSRSTCQPTRVTCRYVKPQRTMHPPQLMEGEALASRV! *F LELGPCSTKFIGPVVMEVPHFASLRGKEREIIILRSDNGETWREHTIDNSEEIIHDVLQQCFEPEEIAQLEEQAGNHVC *F RFVTYDFPQYFAVVSRIRQEVHAIGPEGGMVSSTVVPQVQAVFPQGALTKKIKVGLQAQPVDPDLTAKLLGRGVAVSPI *F VTVEPRRRKFHKAITLSMPAPKAHGQGMINQYSGNTPTLRLLCSITGGPSRAQWEDVTGSTPLTFVNDCVSFTTTVSAR *F FWLMDCRNISDATKMATELYKEVIHVPFIAKFVVFAKKVEPFEARLRVFCMTDDREDKTLEKHELYTEVAKSRDVEVLE *F GKPQYIEMAGNLVPVTKSGDQLQVQFKAFRENRLPFTVRVKDQHADIVGRTLFMKEPKVAKGEPPQQPICILNIVLPEA *F VIPDSTTAFSDRVTSAYRTSMFSLSKHQNDHYIGDIRIVDLSNLLGKDWIQLAPEIGINGEEIDEIINQNTDSIARQAQ *F SMIRLYKDKPNYDILSLETALKNIGRDDIMKKCKSGRLSHSREFDEADLMKNSESVEELVRRESKRIQQINEREEVKYS *F AEEKEVEESESDEEAAKRTVAERREKIVKRLSIERSIPASTQKKEITREITEIKRKSLIEDKKAHHESEILMQLPADNV *F IIKTTTVPDQVIKMKMGKMDSTEVSKSEFDKELTHKFKTSGRSSEEEDQPSSPDQTDKIVQDISAAEKKEKDGVTFSRV *F TTITRQEARDITEDFLEIEKRSQLPATSTTATVHEKFVEEIKEKTSPLASVPQETVKEVQQVISEVTEIASKKVENIIS *F SFESSKSVDATTVLPTQPSVESTKVSETIKNLEDAKAVSAEQVKTVHVVESSSIEETIAEFEAKKVKYDFHGGEPKTQI *F PKFTRKPSDDSMKPTAAPRATVESETESVIETKAEKPISKIPVKTIPTEAQKVSEVDARKITQDFLQGEKLAAEPKPSP *F AASKIPKVEPRKSVDKQVDRESKILDDVVASTATIMTAGLGDE! *F QLKDQLVDHSEIIAKSETVAEKVTELLDTFHKIEEKVTKSKKTSEISSKVEELVKIEEK! *F PLSQVQPKEDKVAEKVAEVIETFHKIEEQITTADARELTRDFLSTEQRNQLPSMPQAAEKPLESSLTSTSASEPESIVT *F VKPSPPASKIPVVEPRKIVFDESTKPLIEPEPVKTTEKKPLNERQLTADFLSMEQQTQLVSEPAKSLVEEVMKSAEQMV *F EQPKQQKSLNERQLTEDFLLMEQQTQLHSDIVKPTDKLIDGIESAAPVGDEASPFHTPKLTTSVVTQESQHLASEYDSD *F TFGKQATIPLGDSKIDQGLTAPVSMEPRKSLTDAEFCKSVGETITKKMSVGVIEISDELKKIESEIPHSQTPPPTPSDN *F KTDKQNEEPELISLERDYLADTVTTLHTTTESTLERVSAITKIGKCKS *F Comments: We determined that there are at least 5 different transcripts *F (labeled a, b, c, d, and e) from the Ank2 gene (CG7462). There results are in *F press: *F M. Hortsch, K.L. Paisley, M.Z. Tian, M. Qian, M. Bouley, and R. Chandler: The *F Axonal Localization of Drosophila Ankyrin 2 Protein Isoforms Is Essential for *F Neuronal Functionality. Mol. Cell. Neurosci. (in press) *F Transcript a is shorter than all the other transcripts, encodes a polypeptide *F of 1159 aa and is identical to the ORF that was reported in our previous paper *F (Bouley et al. 2000). *F A differential splice donor site in exon 9 generates the longer transcripts b, *F c, d and e that encode polypeptides of 2405, 2386, 2451 and 2465 aa *F respectively. These transcripts differ from each other by the differential *F splicing of the small exons 13, 14 and 15 at the 3' end of the large ORF. *F RT-PCR analysis indicates the transcript b ist the most abundant transcript of *F the 4 long transcripts. Above I have pasted the cDNA and protein sequences of *F the long b transcript. I will send you the long transcripts and ORF for the *F other long Ank2 transcripts c, d and e separately. You may want to substitute *F these for the long CG7462 ORF that was predicted by the genome project. *F Please send me an email if you need further explanations or information. *F With the best regards *F Michael Hortsch *F Browser: Mozilla/4.73 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0146856 *a Hortsch *b M. *t 2002.5.1 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Wed May 01 16:35:26 2002 *F To: flybase-updates@morgan.harvard.edu *F Cc: hortsch@umich.edu *F Subject: FlyBase error report for CG7462 on Wed May 1 08:35:13 2002 *F Error report from Michael Hortsch (hortsch@umich.edu) *F Gene or accession: CG7462 *F Release: 2 *F Missed gene *F Gene cDNA sequence: *F > ANTTTTGGAGTTGAAACATNGGAAGGAAAACGGGCAGCAAAACNGAAAAATTGTTCCTTCGAGAACCGTGAACTTAA *F TAAGGGACTGTGTGAGAGCAGCCAGGAGTCCAATCAGACACATACACACACACAAAGACATTTACACCCATACACTGAC *F ACTGACACGCACACAACCGAATCGGTCGCCTGTACGAACACACCCTCTCACACACTCATGCACTCGTGTACATATAGCT *F TACATAAAGTCTAAGGAAAAATCGATAGGAGTGCATTAATAATTCAGCGAAAACGAAACGAGCATAAGGTCTCGAGCAA *F AAATCTTCAAGTAGTGCGAGTTTTCCGCCTCTGCTAACGGTGAAGAAAAGTTCCAGGCCTCGTGAAAAAATATGACTAA *F TCTGCAGTGACGACATACGCGCCGCTTACACAATTTCTCTACACCAATTAACCGCTGAAATCAATTCAAGACGGGCATT *F TTACCATTGGCAATTGTGAAAAAATGGTCACCGAAAATGGAGCACAGGGCGATGGAAACACTTCCTTCCTGCGTGCCGC *F TCGCGCCGGAAATCTGGAGCGAGTGCTCGAGCATTTGAAGAACAACATTGACATTAACACCAGCAATGCGAATGGCTTG *F AATGCCCTGCACCTGGCCTCCAAGGATGGCCACATCCACGTCGTCTCGGAACTCCTTCGTAGGGGCGCGATTGTGGATA *F GTGCCACCAAAAAGGGAAACACTGCCCTCCACATCGCCTCATTGGCTGGACAGGAGGAGGTGGTGAAGCTACTGCTGGA *F GCACAATGCCTCCGTGAATGTGCAATCCCAGAATGGATTCACTCCACTCTACATGGCTGCCCAGGAGAACCACGATGCT *F GTGGTGCGTCTCCTTTTGTCCAACGGAGCCAACCAGAGTCTGGCCACCGAGGATGGCTTCACCCCACTGGCGGTGGCCA *F TGCAGCAGGGTCATGACAAAGTCGTTGCTGTCCTCCTCGAGAG! *F TGATACTCGTGGAAAGGTCAGGCTTCCTGCGCTGCACATTGCCGCCAAAAAGGACGACGTCAAGGCAGCGACTCTGCTT *F CTCGATAATGACCATAATCCGGACGTGACTTCCAAGTCGGGCTTCACCCCGCTGCACATTGCTTCCCATTATGGTAACC *F AAAACATCGCCAACCTACTCATCCAGAAGGGCGCCGATGTCAACTACTCGGCCAAGCACAACATCAGTCCGCTGCATGT *F GGCTGCTAAGTGGGGTAAGACCAACATGGTTTCCCTGCTTTTGGAAAAAGGTGGTAACATCGAGGCAAAGACCCGGGAC *F GGACTTACTCCGCTCCATTGCGCCGCTCGCTCGGGTCATGAGCAAGTTGTTGACATGCTACTCGAACGAGGAGCTCCCA *F TCTCTGCCAAGACCAAAAATGGTTTGGCACCCCTCCATATGGCCGCCCAGGGTGAGCATGTTGATGCCGCCCGCATCCT *F CTTGTACCATCGTGCTCCCGTCGACGAGGTGACAGTGGATTATCTCACTGCTCTTCATGTGGCTGCTCACTGTGGTCAT *F GTTAGGGTGGCTAAGCTTTTACTAGATCGGAATGCTGATGCCAATGCAAGGGCTTTGAATGGATTCACTCCACTGCACA *F TTGCCTGCAAGAAGAACCGCTTGAAGGTGGTAGAGTTGCTCTTGCGACATGGTGCCAGCATTAGTGCCACCACAGAGAG *F TGGACTTACTCCGCTCCATGTGGCCGCCTTTATGGGCTGCATGAACATTGTCATCTATCTGCTGCAGCACGATGCCAGT *F CCCGATGTGCCCACTGTGCGCGGAGAAACGCCACTCCATTTGGCCGCCAGAGCCAACCAGACCGACATCATTCGCATCC *F TGCTGCGCAATGGCGCCCAGGTGGACGCTCGTGCCCGAGAGCAACAGACACCGCTGCACATCGCTTCGCGACTGGGTAA *F TGTAGACATCGTGATGCTTCTGCTGCAACATGGCGCCCAAGTG! *F GACGCAACCACCAAGGACATGTACACGGCACTTCACATCGCTGCCAAGGAGGGCCAGGA! *F TGAGGTGGCCGCTGTACTAATTGAGAACGGCGCCGCCTTGGATGCCGCCACCAAGAAGGGATTCACTCCACTTCACTTG *F ACGGCCAAATATGGACACATTAAGGTTGCCCAGTTGCTGCTCCAAAAGGAGGCAGATGTGGATGCGCAGGGCAAGAACG *F GTGTCACCCCGTTGCATGTGGCTTGCCACTACAATAACCAGCAGGTTGCCTTGTTGCTTTTGGAGAAGGGAGCAAGTCC *F ACATGCCACGGCCAAGAATGGACATACCCCGCTCCATATTGCCGCAAGGAAGAACCAGATGGACATAGCTACTACTCTA *F TTGGAATATGGCGCTTTGGCCAATGCCGAGAGCAAAGCCGGATTTACTCCACTCCATTTGAGCAGCCAGGAGGGTCATG *F CCGAGATCTCCAATCTGTTGATCGAGCACAAGGCCGCCGTCAATCATCCGGCTAAGAATGGTCTAACTCCCATGCATCT *F GTGTGCCCAGGAGGATAATGTGAATGTGGCTGAGATTCTGGAGAAGAACGGCGCCAATATCGACATGGCCACCAAGGCA *F GGCTACACTCCCTTGCATGTGGCCTCTCACTTTGGACAGGCCAACATGGTGCGTTTCTTGCTGCAGAATGGCGCCAATG *F TGGATGCGGCCACCTCAATTGGCTATACTCCGCTCCATCAGACCGCCCAGCAGGGTCATTGTCATATTGTGAATCTCCT *F GCTGGAGCACAAGGCTAATGCCAACGCTCAGACGGTCAATGGACAGACGCCTTTGCACATTGCCCGCAAACTGGGCTAC *F ATAAGTGTCCTAGACTCGTTGAAAACCATCACCAAGGAGGACGAAACAGCTGCTGCTCCTTCCCAAGCCGAGGAGAAGT *F ATCGCGTGGTGGCTCCCGAGGCCATGCACGAATCCTTCATGTCCGACTCCGAGGAAGAGGGCGGCGAAGACAATATGCT *F ATCAGATCAACCATACCGCTATTTGACCGTTGATGAAATGAAA! *F TCCCTAGGCGACGACTCGCTGCCCATCGATGTGACCCGTGATGAGCGCATGGACTCCAACCGGATGACCCAGAGCGCCG *F AGTATGCCTCTGGTGTTCCGCCCACCATTGGGGAGGAGGTGATCAGTCCCCACAAGACCCAAGTCTACGGCAGTTCACC *F CAAGGCCACCGTGGATGGAGTGTACATTGCCAATGGATCTGGTCACGATGAGCCGCCGCATGTGGGTCGCAAGTTAAGC *F TGGAAGAGCTTCTTGGTCTCCTTCCTGGTGGATGCCCGTGGAGGAGCAATGCGCGGCTGTCGGCACAGTGGCGTTCGCA *F TGATCATCCCTTCGAGGTCCACTTGTCAGCCAACCAGGGTAACCTGTCGTTATGTAAAGCCGCAGCGCACAATGCATCC *F GCCCCAGTTGATGGAGGGCGAGGCTTTGGCCAGTCGTGTCCTGGAACTGGGTCCTTGCTCCACCAAGTTTATTGGGCCA *F GTGGTCATGGAGGTGCCACACTTTGCATCGCTGCGTGGCAAGGAGCGCGAGATCATAATATTGCGATCGGACAATGGAG *F AAACATGGCGGGAGCACACCATCGATAACTCCGAGGAGATCATACACGATGTCCTGCAGCAGTGCTTTGAACCAGAGGA *F GATTGCCCAACTGGAGGAGCAGGCTGGCAACCATGTCTGCCGCTTTGTCACCTACGACTTCCCTCAGTACTTTGCCGTC *F GTATCGCGCATACGCCAGGAGGTCCATGCCATTGGACCGGAGGGCGGCATGGTGTCAAGCACCGTGGTGCCACAGGTGC *F AGGCCGTCTTCCCACAGGGAGCCCTCACCAAGAAGATCAAAGTTGGCTTACAGGCCCAACCGGTTGATCCCGATCTCAC *F GGCGAAGCTTTTGGGTCGCGGCGTGGCCGTATCCCCAATTGTTACGGTCGAGCCGCGTCGCCGTAAATTCCACAAGGCC *F ATCACCCTCAGCATGCCCGCCCCCAAGGCTCACGGCCAGGGTA! *F TGATTAACCAGTACTCGGGCAATACGCCCACCCTGCGTCTGCTATGCTCCATTACAGGC! *F GGACCATCTCGTGCTCAATGGGAAGATGTCACCGGTTCCACGCCCTTGACATTTGTCAATGACTGCGTCTCCTTTACGA *F CCACAGTATCAGCTCGTTTCTGGCTGATGGATTGCCGCAACATTTCGGATGCCACCAAAATGGCAACTGAACTGTACAA *F GGAAGTCATCCATGTGCCGTTTATTGCCAAGTTTGTGGTATTCGCCAAGAAGGTAGAACCATTCGAAGCAAGACTCCGA *F GTCTTTTGCATGACCGACGATCGTGAGGACAAAACTCTGGAGAAGCATGAGCTCTACACAGAAGTGGCCAAGAGCCGCG *F ATGTCGAGGTCCTCGAAGGCAAGCCGCAGTACATCGAGATGGCTGGCAATTTGGTGCCAGTGACCAAATCTGGCGACCA *F GCTCCAGGTGCAATTCAAGGCCTTCCGAGAGAACCGCCTACCGTTCACAGTACGAGTCAAGGATCAACATGCCGACATT *F GTTGGACGCACTTTGTTCATGAAGGAACCAAAGGTAGCCAAGGGCGAGCCACCGCAACAGCCCATCTGCATCCTGAACA *F TCGTTCTTCCGGAAGCTGTAATTCCCGATTCCACGACCGCCTTTTCGGACCGCGTCACCTCCGCCTACAGGACCTCAAT *F GTTCAGCTTGAGCAAGCATCAAAATGACCATTACATTGGCGACATCCGCATCGTCGATCTATCCAACCTGTTGGGTAAG *F GATTGGATTCAGCTGGCCCCGGAGATTGGCATCAATGGAGAGGAGATCGATGAGATCATCAACCAAAACACAGATAGCA *F TTGCCAGGCAGGCGCAGAGCATGATTCGCCTGTATAAGGACAAACCTAACTACGATATTCTTTCGCTGGAAACTGCTCT *F CAAAAACATTGGACGAGACGACATCATGAAAAAGTGCAAGAGTGGACGACTATCGCATTCGCGTGAATTCGATGAGGCG *F GACCTTATGAAGAACTCGGAGTCCGTGGAGGAACTGGTTCGCA! *F GGGAAAGCAAGCGAATCCAGCAAATTAACGAACGCGAAGAAGTCAAATACTCTGCCGAGGAGAAGGAGGTCGAGGAATC *F GGAGTCTGATGAAGAAGCTGCCAAGCGAACCGTAGCCGAACGGCGGGAAAAGATTGTTAAGCGTCTGTCCATCGAACGA *F TCAATTCCAGCCTCCACTCAGAAAAAAGAGATCACCCGCGAAATAACCGAAATCAAGCGTAAGAGTCTAATCGAGGACA *F AGAAGGCTCACCATGAGTCCGAAATTCTTATGCAACTGCCTGCGGATAACGTGATTATCAAGACAACAACTGTTCCCGA *F CCAAGTCATTAAGATGAAGATGGGCAAGATGGACTCAACCGAGGTGAGCAAGAGCGAGTTCGACAAGGAACTTACGCAC *F AAGTTCAAGACATCAGGGCGTAGCTCAGAGGAGGAAGACCAGCCATCGTCTCCAGATCAGACTGACAAGATTGTCCAGG *F ACATTAGTGCTGCCGAAAAGAAGGAGAAGGATGGCGTTACCTTCAGCCGAGTGACGACAATAACCCGCCAAGAGGCCCG *F CGACATTACCGAAGACTTCTTAGAGATTGAGAAGCGCAGCCAGCTCCCGGCGACTTCCACAACCGCAACAGTTCATGAG *F AAGTTCGTTGAGGAGATCAAGGAGAAGACCAGCCCTCTGGCTTCAGTGCCACAAGAAACAGTCAAGGAGGTGCAACAAG *F TCATCAGCGAGGTGACCGAAATAGCCAGCAAGAAGGTTGAAAACATTATTAGCTCCTTTGAGAGTAGCAAGTCTGTGGA *F TGCTACTACAGTTCTGCCCACACAGCCGTCTGTTGAATCAACGAAGGTTAGCGAGACCATTAAGAACCTCGAGGATGCC *F AAGGCCGTGTCTGCGGAACAAGTGAAAACTGTCCATGTCGTTGAGAGCTCTAGTATCGAGGAGACTATTGCCGAATTCG *F AAGCCAAGAAGGTTAAATATGACTTCCATGGCGGTGAGCCCAA! *F GACACAGATCCCAAAGTTTACGCGGAAACCAAGCGATGACTCTATGAAGCCCACCGCAG! *F CTCCACGTGCTACAGTTGAATCCGAAACTGAATCGGTCATTGAGACTAAGGCCGAAAAACCGATCTCAAAGATACCAGT *F AAAGACAATACCAACAGAAGCTCAGAAGGTTAGCGAAGTAGATGCACGTAAGATTACTCAAGACTTCCTCCAGGGTGAG *F AAACTGGCGGCCGAACCCAAGCCATCGCCAGCAGCCAGCAAGATACCAAAGGTGGAGCCGAGGAAATCTGTTGATAAGC *F AGGTCGATCGGGAATCAAAAATCTTGGATGATGTTGTGGCCTCTACGGCCACCATTATGACCGCCGGACTGGGAGATGA *F GCAGCTCAAGGATCAACTCGTCGATCACTCAGAAATTATCGCCAAGTCCGAAACAGTCGCTGAAAAGGTAACCGAACTG *F CTTGACACATTCCACAAGATCGAGGAGAAGGTCACCAAATCTAAAAAGACCTCGGAAATATCGAGCAAAGTGGAGGAAC *F TGGTTAAAATCGAGGAAAAACCTCTCTCACAAGTTCAACCCAAGGAGGACAAGGTGGCGGAAAAGGTTGCTGAAGTTAT *F CGAAACATTCCACAAAATCGAGGAGCAGATCACCACTGCCGATGCCCGCGAGCTAACTCGTGATTTCCTGAGCACGGAA *F CAACGAAACCAATTGCCGAGCATGCCACAAGCTGCTGAGAAGCCCCTTGAATCTAGCTTGACATCCACCAGTGCCTCGG *F AACCAGAATCAATAGTCACAGTGAAGCCATCACCCCCAGCGAGTAAGATACCTGTAGTGGAACCCAGGAAAATCGTTTT *F CGACGAATCTACAAAGCCTCTGATCGAGCCTGAGCCAGTGAAGACCACTGAAAAGAAGCCTCTTAATGAGCGACAACTA *F ACCGCTGATTTCTTGAGCATGGAGCAACAAACCCAGCTGGTCTCTGAGCCTGCCAAATCGTTGGTGGAAGAGGTGATGA *F AGTCCGCCGAACAAATGGTGGAGCAACCGAAACAGCAAAAATC! *F CCTGAATGAGCGACAACTTACAGAGGACTTTCTGCTTATGGAACAGCAGACACAACTGCATTCAGACATTGTTAAGCCA *F ACGGATAAGCTGATCGATGGTATTGAGTCCGCAGCGCCGGTTGGAGATGAAGCATCACCGTTCCACACGCCCAAGCTGA *F CCACCAGTGTGGTGACACAGGAGTCACAGCACCTGGCGAGTGAATATGACAGTGACACTTTCGGCAAACAGGCCACCAT *F ACCGTTAGGAGACTCAAAGATTGATCAGGGACTCACTGCACCAGTATCCATGGAACCACGAAAGTCACTGACCGATGCC *F GAGTTCTGCAAATCGGTGGGCGAAACAATCACCAAGAAGATGAGTGTGGGAGTTATTGAAATAAGTGATGAGCTCAAGA *F AAATTGGTACGTTGCCCAGAGTTTCTTTAAGTTATTATCTAGTCTTACCTAATTTTTCGATGCAGAGAGCGAAATTCCA *F CATTCCCAGACTCCTCCGCCAACACCGAGTGACAACAAAACCGACAAGCAGAACGAAGAGCCAGAACTGA *F Protein sequence: *F > MVTENGAQGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKG *F NTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHD *F KVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSA *F KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQG *F EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHG *F ASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTP *F LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVA *F QLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAE *F SKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASH *F FGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTI *F TKEDETAAAPSQAEEKYRVVAPEAMHESFMSDSEEEGGEDNMLSDQPYRYLTVDEMKSLGDDSLPIDVTRDERMDSNRM *F TQSAEYASGVPPTIGEEVISPHKTQVYGSSPKATVDGVYIANGSGHDEPPHVGRKLSWKSFLVSFLVDARGGAMRGCRH *F SGVRMIIPSRSTCQPTRVTCRYVKPQRTMHPPQLMEGEALASR! *F VLELGPCSTKFIGPVVMEVPHFASLRGKEREIIILRSDNGETWREHTIDNSEEIIHDVLQQCFEPEEIAQLEEQAGNHV *F CRFVTYDFPQYFAVVSRIRQEVHAIGPEGGMVSSTVVPQVQAVFPQGALTKKIKVGLQAQPVDPDLTAKLLGRGVAVSP *F IVTVEPRRRKFHKAITLSMPAPKAHGQGMINQYSGNTPTLRLLCSITGGPSRAQWEDVTGSTPLTFVNDCVSFTTTVSA *F RFWLMDCRNISDATKMATELYKEVIHVPFIAKFVVFAKKVEPFEARLRVFCMTDDREDKTLEKHELYTEVAKSRDVEVL *F EGKPQYIEMAGNLVPVTKSGDQLQVQFKAFRENRLPFTVRVKDQHADIVGRTLFMKEPKVAKGEPPQQPICILNIVLPE *F AVIPDSTTAFSDRVTSAYRTSMFSLSKHQNDHYIGDIRIVDLSNLLGKDWIQLAPEIGINGEEIDEIINQNTDSIARQA *F QSMIRLYKDKPNYDILSLETALKNIGRDDIMKKCKSGRLSHSREFDEADLMKNSESVEELVRRESKRIQQINEREEVKY *F SAEEKEVEESESDEEAAKRTVAERREKIVKRLSIERSIPASTQKKEITREITEIKRKSLIEDKKAHHESEILMQLPADN *F VIIKTTTVPDQVIKMKMGKMDSTEVSKSEFDKELTHKFKTSGRSSEEEDQPSSPDQTDKIVQDISAAEKKEKDGVTFSR *F VTTITRQEARDITEDFLEIEKRSQLPATSTTATVHEKFVEEIKEKTSPLASVPQETVKEVQQVISEVTEIASKKVENII *F SSFESSKSVDATTVLPTQPSVESTKVSETIKNLEDAKAVSAEQVKTVHVVESSSIEETIAEFEAKKVKYDFHGGEPKTQ *F IPKFTRKPSDDSMKPTAAPRATVESETESVIETKAEKPISKIPVKTIPTEAQKVSEVDARKITQDFLQGEKLAAEPKPS *F PAASKIPKVEPRKSVDKQVDRESKILDDVVASTATIMTAGLGD! *F EQLKDQLVDHSEIIAKSETVAEKVTELLDTFHKIEEKVTKSKKTSEISSKVEELVKIEE! *F KPLSQVQPKEDKVAEKVAEVIETFHKIEEQITTADARELTRDFLSTEQRNQLPSMPQAAEKPLESSLTSTSASEPESIV *F TVKPSPPASKIPVVEPRKIVFDESTKPLIEPEPVKTTEKKPLNERQLTADFLSMEQQTQLVSEPAKSLVEEVMKSAEQM *F VEQPKQQKSLNERQLTEDFLLMEQQTQLHSDIVKPTDKLIDGIESAAPVGDEASPFHTPKLTTSVVTQESQHLASEYDS *F DTFGKQATIPLGDSKIDQGLTAPVSMEPRKSLTDAEFCKSVGETITKKMSVGVIEISDELKKIGTLPRVSLSYYLVLPN *F FSMQRAKFHIPRLLRQHRVTTKPTSRTKSQN *F Comments: Here I am sending you the Ank2 (CG7462) long c transcript with its *F ORF. *F Read my explanation in the previous message. *F Browser: Mozilla/4.73 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0146857 *a Hortsch *b M. *t 2002.5.1 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Wed May 01 16:36:15 2002 *F To: flybase-updates@morgan.harvard.edu *F Cc: hortsch@umich.edu *F Subject: FlyBase error report for CG7462 on Wed May 1 08:36:15 2002 *F Error report from Michael Hortsch (hortsch@umich.edu) *F Gene or accession: CG7462 *F Release: 2 *F Missed gene *F Gene cDNA sequence: *F > ANTTTTGGAGTTGAAACATNGGAAGGAAAACGGGCAGCAAAACNGAAAAATTGTTCCTTCGAGAACCGTGAACTTAA *F TAAGGGACTGTGTGAGAGCAGCCAGGAGTCCAATCAGACACATACACACACACAAAGACATTTACACCCATACACTGAC *F ACTGACACGCACACAACCGAATCGGTCGCCTGTACGAACACACCCTCTCACACACTCATGCACTCGTGTACATATAGCT *F TACATAAAGTCTAAGGAAAAATCGATAGGAGTGCATTAATAATTCAGCGAAAACGAAACGAGCATAAGGTCTCGAGCAA *F AAATCTTCAAGTAGTGCGAGTTTTCCGCCTCTGCTAACGGTGAAGAAAAGTTCCAGGCCTCGTGAAAAAATATGACTAA *F TCTGCAGTGACGACATACGCGCCGCTTACACAATTTCTCTACACCAATTAACCGCTGAAATCAATTCAAGACGGGCATT *F TTACCATTGGCAATTGTGAAAAAATGGTCACCGAAAATGGAGCACAGGGCGATGGAAACACTTCCTTCCTGCGTGCCGC *F TCGCGCCGGAAATCTGGAGCGAGTGCTCGAGCATTTGAAGAACAACATTGACATTAACACCAGCAATGCGAATGGCTTG *F AATGCCCTGCACCTGGCCTCCAAGGATGGCCACATCCACGTCGTCTCGGAACTCCTTCGTAGGGGCGCGATTGTGGATA *F GTGCCACCAAAAAGGGAAACACTGCCCTCCACATCGCCTCATTGGCTGGACAGGAGGAGGTGGTGAAGCTACTGCTGGA *F GCACAATGCCTCCGTGAATGTGCAATCCCAGAATGGATTCACTCCACTCTACATGGCTGCCCAGGAGAACCACGATGCT *F GTGGTGCGTCTCCTTTTGTCCAACGGAGCCAACCAGAGTCTGGCCACCGAGGATGGCTTCACCCCACTGGCGGTGGCCA *F TGCAGCAGGGTCATGACAAAGTCGTTGCTGTCCTCCTCGAGAG! *F TGATACTCGTGGAAAGGTCAGGCTTCCTGCGCTGCACATTGCCGCCAAAAAGGACGACGTCAAGGCAGCGACTCTGCTT *F CTCGATAATGACCATAATCCGGACGTGACTTCCAAGTCGGGCTTCACCCCGCTGCACATTGCTTCCCATTATGGTAACC *F AAAACATCGCCAACCTACTCATCCAGAAGGGCGCCGATGTCAACTACTCGGCCAAGCACAACATCAGTCCGCTGCATGT *F GGCTGCTAAGTGGGGTAAGACCAACATGGTTTCCCTGCTTTTGGAAAAAGGTGGTAACATCGAGGCAAAGACCCGGGAC *F GGACTTACTCCGCTCCATTGCGCCGCTCGCTCGGGTCATGAGCAAGTTGTTGACATGCTACTCGAACGAGGAGCTCCCA *F TCTCTGCCAAGACCAAAAATGGTTTGGCACCCCTCCATATGGCCGCCCAGGGTGAGCATGTTGATGCCGCCCGCATCCT *F CTTGTACCATCGTGCTCCCGTCGACGAGGTGACAGTGGATTATCTCACTGCTCTTCATGTGGCTGCTCACTGTGGTCAT *F GTTAGGGTGGCTAAGCTTTTACTAGATCGGAATGCTGATGCCAATGCAAGGGCTTTGAATGGATTCACTCCACTGCACA *F TTGCCTGCAAGAAGAACCGCTTGAAGGTGGTAGAGTTGCTCTTGCGACATGGTGCCAGCATTAGTGCCACCACAGAGAG *F TGGACTTACTCCGCTCCATGTGGCCGCCTTTATGGGCTGCATGAACATTGTCATCTATCTGCTGCAGCACGATGCCAGT *F CCCGATGTGCCCACTGTGCGCGGAGAAACGCCACTCCATTTGGCCGCCAGAGCCAACCAGACCGACATCATTCGCATCC *F TGCTGCGCAATGGCGCCCAGGTGGACGCTCGTGCCCGAGAGCAACAGACACCGCTGCACATCGCTTCGCGACTGGGTAA *F TGTAGACATCGTGATGCTTCTGCTGCAACATGGCGCCCAAGTG! *F GACGCAACCACCAAGGACATGTACACGGCACTTCACATCGCTGCCAAGGAGGGCCAGGA! *F TGAGGTGGCCGCTGTACTAATTGAGAACGGCGCCGCCTTGGATGCCGCCACCAAGAAGGGATTCACTCCACTTCACTTG *F ACGGCCAAATATGGACACATTAAGGTTGCCCAGTTGCTGCTCCAAAAGGAGGCAGATGTGGATGCGCAGGGCAAGAACG *F GTGTCACCCCGTTGCATGTGGCTTGCCACTACAATAACCAGCAGGTTGCCTTGTTGCTTTTGGAGAAGGGAGCAAGTCC *F ACATGCCACGGCCAAGAATGGACATACCCCGCTCCATATTGCCGCAAGGAAGAACCAGATGGACATAGCTACTACTCTA *F TTGGAATATGGCGCTTTGGCCAATGCCGAGAGCAAAGCCGGATTTACTCCACTCCATTTGAGCAGCCAGGAGGGTCATG *F CCGAGATCTCCAATCTGTTGATCGAGCACAAGGCCGCCGTCAATCATCCGGCTAAGAATGGTCTAACTCCCATGCATCT *F GTGTGCCCAGGAGGATAATGTGAATGTGGCTGAGATTCTGGAGAAGAACGGCGCCAATATCGACATGGCCACCAAGGCA *F GGCTACACTCCCTTGCATGTGGCCTCTCACTTTGGACAGGCCAACATGGTGCGTTTCTTGCTGCAGAATGGCGCCAATG *F TGGATGCGGCCACCTCAATTGGCTATACTCCGCTCCATCAGACCGCCCAGCAGGGTCATTGTCATATTGTGAATCTCCT *F GCTGGAGCACAAGGCTAATGCCAACGCTCAGACGGTCAATGGACAGACGCCTTTGCACATTGCCCGCAAACTGGGCTAC *F ATAAGTGTCCTAGACTCGTTGAAAACCATCACCAAGGAGGACGAAACAGCTGCTGCTCCTTCCCAAGCCGAGGAGAAGT *F ATCGCGTGGTGGCTCCCGAGGCCATGCACGAATCCTTCATGTCCGACTCCGAGGAAGAGGGCGGCGAAGACAATATGCT *F ATCAGATCAACCATACCGCTATTTGACCGTTGATGAAATGAAA! *F TCCCTAGGCGACGACTCGCTGCCCATCGATGTGACCCGTGATGAGCGCATGGACTCCAACCGGATGACCCAGAGCGCCG *F AGTATGCCTCTGGTGTTCCGCCCACCATTGGGGAGGAGGTGATCAGTCCCCACAAGACCCAAGTCTACGGCAGTTCACC *F CAAGGCCACCGTGGATGGAGTGTACATTGCCAATGGATCTGGTCACGATGAGCCGCCGCATGTGGGTCGCAAGTTAAGC *F TGGAAGAGCTTCTTGGTCTCCTTCCTGGTGGATGCCCGTGGAGGAGCAATGCGCGGCTGTCGGCACAGTGGCGTTCGCA *F TGATCATCCCTTCGAGGTCCACTTGTCAGCCAACCAGGGTAACCTGTCGTTATGTAAAGCCGCAGCGCACAATGCATCC *F GCCCCAGTTGATGGAGGGCGAGGCTTTGGCCAGTCGTGTCCTGGAACTGGGTCCTTGCTCCACCAAGTTTATTGGGCCA *F GTGGTCATGGAGGTGCCACACTTTGCATCGCTGCGTGGCAAGGAGCGCGAGATCATAATATTGCGATCGGACAATGGAG *F AAACATGGCGGGAGCACACCATCGATAACTCCGAGGAGATCATACACGATGTCCTGCAGCAGTGCTTTGAACCAGAGGA *F GATTGCCCAACTGGAGGAGCAGGCTGGCAACCATGTCTGCCGCTTTGTCACCTACGACTTCCCTCAGTACTTTGCCGTC *F GTATCGCGCATACGCCAGGAGGTCCATGCCATTGGACCGGAGGGCGGCATGGTGTCAAGCACCGTGGTGCCACAGGTGC *F AGGCCGTCTTCCCACAGGGAGCCCTCACCAAGAAGATCAAAGTTGGCTTACAGGCCCAACCGGTTGATCCCGATCTCAC *F GGCGAAGCTTTTGGGTCGCGGCGTGGCCGTATCCCCAATTGTTACGGTCGAGCCGCGTCGCCGTAAATTCCACAAGGCC *F ATCACCCTCAGCATGCCCGCCCCCAAGGCTCACGGCCAGGGTA! *F TGATTAACCAGTACTCGGGCAATACGCCCACCCTGCGTCTGCTATGCTCCATTACAGGC! *F GGACCATCTCGTGCTCAATGGGAAGATGTCACCGGTTCCACGCCCTTGACATTTGTCAATGACTGCGTCTCCTTTACGA *F CCACAGTATCAGCTCGTTTCTGGCTGATGGATTGCCGCAACATTTCGGATGCCACCAAAATGGCAACTGAACTGTACAA *F GGAAGTCATCCATGTGCCGTTTATTGCCAAGTTTGTGGTATTCGCCAAGAAGGTAGAACCATTCGAAGCAAGACTCCGA *F GTCTTTTGCATGACCGACGATCGTGAGGACAAAACTCTGGAGAAGCATGAGCTCTACACAGAAGTGGCCAAGAGCCGCG *F ATGTCGAGGTCCTCGAAGGCAAGCCGCAGTACATCGAGATGGCTGGCAATTTGGTGCCAGTGACCAAATCTGGCGACCA *F GCTCCAGGTGCAATTCAAGGCCTTCCGAGAGAACCGCCTACCGTTCACAGTACGAGTCAAGGATCAACATGCCGACATT *F GTTGGACGCACTTTGTTCATGAAGGAACCAAAGGTAGCCAAGGGCGAGCCACCGCAACAGCCCATCTGCATCCTGAACA *F TCGTTCTTCCGGAAGCTGTAATTCCCGATTCCACGACCGCCTTTTCGGACCGCGTCACCTCCGCCTACAGGACCTCAAT *F GTTCAGCTTGAGCAAGCATCAAAATGACCATTACATTGGCGACATCCGCATCGTCGATCTATCCAACCTGTTGGGTAAG *F GATTGGATTCAGCTGGCCCCGGAGATTGGCATCAATGGAGAGGAGATCGATGAGATCATCAACCAAAACACAGATAGCA *F TTGCCAGGCAGGCGCAGAGCATGATTCGCCTGTATAAGGACAAACCTAACTACGATATTCTTTCGCTGGAAACTGCTCT *F CAAAAACATTGGACGAGACGACATCATGAAAAAGTGCAAGAGTGGACGACTATCGCATTCGCGTGAATTCGATGAGGCG *F GACCTTATGAAGAACTCGGAGTCCGTGGAGGAACTGGTTCGCA! *F GGGAAAGCAAGCGAATCCAGCAAATTAACGAACGCGAAGAAGTCAAATACTCTGCCGAGGAGAAGGAGGTCGAGGAATC *F GGAGTCTGATGAAGAAGCTGCCAAGCGAACCGTAGCCGAACGGCGGGAAAAGATTGTTAAGCGTCTGTCCATCGAACGA *F TCAATTCCAGCCTCCACTCAGAAAAAAGAGATCACCCGCGAAATAACCGAAATCAAGCGTAAGAGTCTAATCGAGGACA *F AGAAGGCTCACCATGAGTCCGAAATTCTTATGCAACTGCCTGCGGATAACGTGATTATCAAGACAACAACTGTTCCCGA *F CCAAGTCATTAAGATGAAGATGGGCAAGATGGACTCAACCGAGGTGAGCAAGAGCGAGTTCGACAAGGAACTTACGCAC *F AAGTTCAAGACATCAGGGCGTAGCTCAGAGGAGGAAGACCAGCCATCGTCTCCAGATCAGACTGACAAGATTGTCCAGG *F ACATTAGTGCTGCCGAAAAGAAGGAGAAGGATGGCGTTACCTTCAGCCGAGTGACGACAATAACCCGCCAAGAGGCCCG *F CGACATTACCGAAGACTTCTTAGAGATTGAGAAGCGCAGCCAGCTCCCGGCGACTTCCACAACCGCAACAGTTCATGAG *F AAGTTCGTTGAGGAGATCAAGGAGAAGACCAGCCCTCTGGCTTCAGTGCCACAAGAAACAGTCAAGGAGGTGCAACAAG *F TCATCAGCGAGGTGACCGAAATAGCCAGCAAGAAGGTTGAAAACATTATTAGCTCCTTTGAGAGTAGCAAGTCTGTGGA *F TGCTACTACAGTTCTGCCCACACAGCCGTCTGTTGAATCAACGAAGGTTAGCGAGACCATTAAGAACCTCGAGGATGCC *F AAGGCCGTGTCTGCGGAACAAGTGAAAACTGTCCATGTCGTTGAGAGCTCTAGTATCGAGGAGACTATTGCCGAATTCG *F AAGCCAAGAAGGTTAAATATGACTTCCATGGCGGTGAGCCCAA! *F GACACAGATCCCAAAGTTTACGCGGAAACCAAGCGATGACTCTATGAAGCCCACCGCAG! *F CTCCACGTGCTACAGTTGAATCCGAAACTGAATCGGTCATTGAGACTAAGGCCGAAAAACCGATCTCAAAGATACCAGT *F AAAGACAATACCAACAGAAGCTCAGAAGGTTAGCGAAGTAGATGCACGTAAGATTACTCAAGACTTCCTCCAGGGTGAG *F AAACTGGCGGCCGAACCCAAGCCATCGCCAGCAGCCAGCAAGATACCAAAGGTGGAGCCGAGGAAATCTGTTGATAAGC *F AGGTCGATCGGGAATCAAAAATCTTGGATGATGTTGTGGCCTCTACGGCCACCATTATGACCGCCGGACTGGGAGATGA *F GCAGCTCAAGGATCAACTCGTCGATCACTCAGAAATTATCGCCAAGTCCGAAACAGTCGCTGAAAAGGTAACCGAACTG *F CTTGACACATTCCACAAGATCGAGGAGAAGGTCACCAAATCTAAAAAGACCTCGGAAATATCGAGCAAAGTGGAGGAAC *F TGGTTAAAATCGAGGAAAAACCTCTCTCACAAGTTCAACCCAAGGAGGACAAGGTGGCGGAAAAGGTTGCTGAAGTTAT *F CGAAACATTCCACAAAATCGAGGAGCAGATCACCACTGCCGATGCCCGCGAGCTAACTCGTGATTTCCTGAGCACGGAA *F CAACGAAACCAATTGCCGAGCATGCCACAAGCTGCTGAGAAGCCCCTTGAATCTAGCTTGACATCCACCAGTGCCTCGG *F AACCAGAATCAATAGTCACAGTGAAGCCATCACCCCCAGCGAGTAAGATACCTGTAGTGGAACCCAGGAAAATCGTTTT *F CGACGAATCTACAAAGCCTCTGATCGAGCCTGAGCCAGTGAAGACCACTGAAAAGAAGCCTCTTAATGAGCGACAACTA *F ACCGCTGATTTCTTGAGCATGGAGCAACAAACCCAGCTGGTCTCTGAGCCTGCCAAATCGTTGGTGGAAGAGGTGATGA *F AGTCCGCCGAACAAATGGTGGAGCAACCGAAACAGCAAAAATC! *F CCTGAATGAGCGACAACTTACAGAGGACTTTCTGCTTATGGAACAGCAGACACAACTGCATTCAGACATTGTTAAGCCA *F ACGGATAAGCTGATCGATGGTATTGAGTCCGCAGCGCCGGTTGGAGATGAAGCATCACCGTTCCACACGCCCAAGCTGA *F CCACCAGTGTGGTGACACAGGAGTCACAGCACCTGGCGAGTGAATATGACAGTGACACTTTCGGCAAACAGGCCACCAT *F ACCGTTAGGAGACTCAAAGATTGATCAGGGACTCACTGCACCAGTATCCATGGAACCACGAAAGTCACTGACCGATGCC *F GAGTTCTGCAAATCGGTGGGCGAAACAATCACCAAGAAGATGAGTGTGGGAGTTATTGAAATAAGTGATGAGCTCAAGA *F AAATTGAGAGCGAAATTCCACATTCCCAGACTCCTCCGCCAACACCGAGTGACAACAAAACCGACAAGCAGAACGAAGA *F GCCAGAACTGATCAGCTTGGAACGTGATTACCTGGAGAGCGAAATTCCACATTCCCAGACTCCTCCGCCAACACCGAGT *F GACAACAAAACCGACAAGCAGAACGAAGAGCCAGAACTGATCAGCTTGGAACGTGGTAAGTTGGTGTTGGTAACGCTAA *F TGCTTCGATATATACAACAATTTGAAAATAGATTACCTGGCCGACACTGTGACCACCCTTCACACAACTACAGAGTCGA *F CCCTCGAACGCGTTTCTGCCATTACTAA *F Protein sequence: *F > MVTENGAQGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKG *F NTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHD *F KVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSA *F KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQG *F EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHG *F ASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTP *F LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVA *F QLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAE *F SKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASH *F FGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTI *F TKEDETAAAPSQAEEKYRVVAPEAMHESFMSDSEEEGGEDNMLSDQPYRYLTVDEMKSLGDDSLPIDVTRDERMDSNRM *F TQSAEYASGVPPTIGEEVISPHKTQVYGSSPKATVDGVYIANGSGHDEPPHVGRKLSWKSFLVSFLVDARGGAMRGCRH *F SGVRMIIPSRSTCQPTRVTCRYVKPQRTMHPPQLMEGEALASR! *F VLELGPCSTKFIGPVVMEVPHFASLRGKEREIIILRSDNGETWREHTIDNSEEIIHDVLQQCFEPEEIAQLEEQAGNHV *F CRFVTYDFPQYFAVVSRIRQEVHAIGPEGGMVSSTVVPQVQAVFPQGALTKKIKVGLQAQPVDPDLTAKLLGRGVAVSP *F IVTVEPRRRKFHKAITLSMPAPKAHGQGMINQYSGNTPTLRLLCSITGGPSRAQWEDVTGSTPLTFVNDCVSFTTTVSA *F RFWLMDCRNISDATKMATELYKEVIHVPFIAKFVVFAKKVEPFEARLRVFCMTDDREDKTLEKHELYTEVAKSRDVEVL *F EGKPQYIEMAGNLVPVTKSGDQLQVQFKAFRENRLPFTVRVKDQHADIVGRTLFMKEPKVAKGEPPQQPICILNIVLPE *F AVIPDSTTAFSDRVTSAYRTSMFSLSKHQNDHYIGDIRIVDLSNLLGKDWIQLAPEIGINGEEIDEIINQNTDSIARQA *F QSMIRLYKDKPNYDILSLETALKNIGRDDIMKKCKSGRLSHSREFDEADLMKNSESVEELVRRESKRIQQINEREEVKY *F SAEEKEVEESESDEEAAKRTVAERREKIVKRLSIERSIPASTQKKEITREITEIKRKSLIEDKKAHHESEILMQLPADN *F VIIKTTTVPDQVIKMKMGKMDSTEVSKSEFDKELTHKFKTSGRSSEEEDQPSSPDQTDKIVQDISAAEKKEKDGVTFSR *F VTTITRQEARDITEDFLEIEKRSQLPATSTTATVHEKFVEEIKEKTSPLASVPQETVKEVQQVISEVTEIASKKVENII *F SSFESSKSVDATTVLPTQPSVESTKVSETIKNLEDAKAVSAEQVKTVHVVESSSIEETIAEFEAKKVKYDFHGGEPKTQ *F IPKFTRKPSDDSMKPTAAPRATVESETESVIETKAEKPISKIPVKTIPTEAQKVSEVDARKITQDFLQGEKLAAEPKPS *F PAASKIPKVEPRKSVDKQVDRESKILDDVVASTATIMTAGLGD! *F EQLKDQLVDHSEIIAKSETVAEKVTELLDTFHKIEEKVTKSKKTSEISSKVEELVKIEE! *F KPLSQVQPKEDKVAEKVAEVIETFHKIEEQITTADARELTRDFLSTEQRNQLPSMPQAAEKPLESSLTSTSASEPESIV *F TVKPSPPASKIPVVEPRKIVFDESTKPLIEPEPVKTTEKKPLNERQLTADFLSMEQQTQLVSEPAKSLVEEVMKSAEQM *F VEQPKQQKSLNERQLTEDFLLMEQQTQLHSDIVKPTDKLIDGIESAAPVGDEASPFHTPKLTTSVVTQESQHLASEYDS *F DTFGKQATIPLGDSKIDQGLTAPVSMEPRKSLTDAEFCKSVGETITKKMSVGVIEISDELKKIESEIPHSQTPPPTPSD *F NKTDKQNEEPELISLERDYLESEIPHSQTPPPTPSDNKTDKQNEEPELISLERGKLVLVTLMLRYIQQFENRLPGRHCD *F HPSHNYRVDPRTRFCHY *F Comments: Here I am sending you the Ank2 (CG7462) long d transcript with its *F ORF. *F Read my explanation in the previous message. *F Browser: Mozilla/4.73 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0146858 *a Hortsch *b M. *t 2002.5.1 *T personal communication to FlyBase *u *F From FlyBase-error@hedgehog.lbl.gov Wed May 01 16:37:15 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 1 May 2002 16:37:15 \+0100 *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 1 May 2002 08:37:15 \-0700 (PDT) *F X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error *F using \-f *F To: flybase-updates@morgan.harvard.edu *F Cc: hortsch@umich.edu *F Subject: FlyBase error report for CG7462 on Wed May 1 08:37:15 2002 *F X-Virus-Scanned: by AMaViS perl-11 *F Content-Length: 10753 *F Error report from Michael Hortsch (hortsch@umich.edu) *F Gene or accession: CG7462 *F Release: 2 *F Missed gene *F Gene cDNA sequence: *F > ANTTTTGGAGTTGAAACATNGGAAGGAAAACGGGCAGCAAAACNGAAAAATTGTTCCTTCGAGAACCGTGAACTTAA *F TAAGGGACTGTGTGAGAGCAGCCAGGAGTCCAATCAGACACATACACACACACAAAGACATTTACACCCATACACTGAC *F ACTGACACGCACACAACCGAATCGGTCGCCTGTACGAACACACCCTCTCACACACTCATGCACTCGTGTACATATAGCT *F TACATAAAGTCTAAGGAAAAATCGATAGGAGTGCATTAATAATTCAGCGAAAACGAAACGAGCATAAGGTCTCGAGCAA *F AAATCTTCAAGTAGTGCGAGTTTTCCGCCTCTGCTAACGGTGAAGAAAAGTTCCAGGCCTCGTGAAAAAATATGACTAA *F TCTGCAGTGACGACATACGCGCCGCTTACACAATTTCTCTACACCAATTAACCGCTGAAATCAATTCAAGACGGGCATT *F TTACCATTGGCAATTGTGAAAAAATGGTCACCGAAAATGGAGCACAGGGCGATGGAAACACTTCCTTCCTGCGTGCCGC *F TCGCGCCGGAAATCTGGAGCGAGTGCTCGAGCATTTGAAGAACAACATTGACATTAACACCAGCAATGCGAATGGCTTG *F AATGCCCTGCACCTGGCCTCCAAGGATGGCCACATCCACGTCGTCTCGGAACTCCTTCGTAGGGGCGCGATTGTGGATA *F GTGCCACCAAAAAGGGAAACACTGCCCTCCACATCGCCTCATTGGCTGGACAGGAGGAGGTGGTGAAGCTACTGCTGGA *F GCACAATGCCTCCGTGAATGTGCAATCCCAGAATGGATTCACTCCACTCTACATGGCTGCCCAGGAGAACCACGATGCT *F GTGGTGCGTCTCCTTTTGTCCAACGGAGCCAACCAGAGTCTGGCCACCGAGGATGGCTTCACCCCACTGGCGGTGGCCA *F TGCAGCAGGGTCATGACAAAGTCGTTGCTGTCCTCCTCGAGAG! *F TGATACTCGTGGAAAGGTCAGGCTTCCTGCGCTGCACATTGCCGCCAAAAAGGACGACGTCAAGGCAGCGACTCTGCTT *F CTCGATAATGACCATAATCCGGACGTGACTTCCAAGTCGGGCTTCACCCCGCTGCACATTGCTTCCCATTATGGTAACC *F AAAACATCGCCAACCTACTCATCCAGAAGGGCGCCGATGTCAACTACTCGGCCAAGCACAACATCAGTCCGCTGCATGT *F GGCTGCTAAGTGGGGTAAGACCAACATGGTTTCCCTGCTTTTGGAAAAAGGTGGTAACATCGAGGCAAAGACCCGGGAC *F GGACTTACTCCGCTCCATTGCGCCGCTCGCTCGGGTCATGAGCAAGTTGTTGACATGCTACTCGAACGAGGAGCTCCCA *F TCTCTGCCAAGACCAAAAATGGTTTGGCACCCCTCCATATGGCCGCCCAGGGTGAGCATGTTGATGCCGCCCGCATCCT *F CTTGTACCATCGTGCTCCCGTCGACGAGGTGACAGTGGATTATCTCACTGCTCTTCATGTGGCTGCTCACTGTGGTCAT *F GTTAGGGTGGCTAAGCTTTTACTAGATCGGAATGCTGATGCCAATGCAAGGGCTTTGAATGGATTCACTCCACTGCACA *F TTGCCTGCAAGAAGAACCGCTTGAAGGTGGTAGAGTTGCTCTTGCGACATGGTGCCAGCATTAGTGCCACCACAGAGAG *F TGGACTTACTCCGCTCCATGTGGCCGCCTTTATGGGCTGCATGAACATTGTCATCTATCTGCTGCAGCACGATGCCAGT *F CCCGATGTGCCCACTGTGCGCGGAGAAACGCCACTCCATTTGGCCGCCAGAGCCAACCAGACCGACATCATTCGCATCC *F TGCTGCGCAATGGCGCCCAGGTGGACGCTCGTGCCCGAGAGCAACAGACACCGCTGCACATCGCTTCGCGACTGGGTAA *F TGTAGACATCGTGATGCTTCTGCTGCAACATGGCGCCCAAGTG! *F GACGCAACCACCAAGGACATGTACACGGCACTTCACATCGCTGCCAAGGAGGGCCAGGA! *F TGAGGTGGCCGCTGTACTAATTGAGAACGGCGCCGCCTTGGATGCCGCCACCAAGAAGGGATTCACTCCACTTCACTTG *F ACGGCCAAATATGGACACATTAAGGTTGCCCAGTTGCTGCTCCAAAAGGAGGCAGATGTGGATGCGCAGGGCAAGAACG *F GTGTCACCCCGTTGCATGTGGCTTGCCACTACAATAACCAGCAGGTTGCCTTGTTGCTTTTGGAGAAGGGAGCAAGTCC *F ACATGCCACGGCCAAGAATGGACATACCCCGCTCCATATTGCCGCAAGGAAGAACCAGATGGACATAGCTACTACTCTA *F TTGGAATATGGCGCTTTGGCCAATGCCGAGAGCAAAGCCGGATTTACTCCACTCCATTTGAGCAGCCAGGAGGGTCATG *F CCGAGATCTCCAATCTGTTGATCGAGCACAAGGCCGCCGTCAATCATCCGGCTAAGAATGGTCTAACTCCCATGCATCT *F GTGTGCCCAGGAGGATAATGTGAATGTGGCTGAGATTCTGGAGAAGAACGGCGCCAATATCGACATGGCCACCAAGGCA *F GGCTACACTCCCTTGCATGTGGCCTCTCACTTTGGACAGGCCAACATGGTGCGTTTCTTGCTGCAGAATGGCGCCAATG *F TGGATGCGGCCACCTCAATTGGCTATACTCCGCTCCATCAGACCGCCCAGCAGGGTCATTGTCATATTGTGAATCTCCT *F GCTGGAGCACAAGGCTAATGCCAACGCTCAGACGGTCAATGGACAGACGCCTTTGCACATTGCCCGCAAACTGGGCTAC *F ATAAGTGTCCTAGACTCGTTGAAAACCATCACCAAGGAGGACGAAACAGCTGCTGCTCCTTCCCAAGCCGAGGAGAAGT *F ATCGCGTGGTGGCTCCCGAGGCCATGCACGAATCCTTCATGTCCGACTCCGAGGAAGAGGGCGGCGAAGACAATATGCT *F ATCAGATCAACCATACCGCTATTTGACCGTTGATGAAATGAAA! *F TCCCTAGGCGACGACTCGCTGCCCATCGATGTGACCCGTGATGAGCGCATGGACTCCAACCGGATGACCCAGAGCGCCG *F AGTATGCCTCTGGTGTTCCGCCCACCATTGGGGAGGAGGTGATCAGTCCCCACAAGACCCAAGTCTACGGCAGTTCACC *F CAAGGCCACCGTGGATGGAGTGTACATTGCCAATGGATCTGGTCACGATGAGCCGCCGCATGTGGGTCGCAAGTTAAGC *F TGGAAGAGCTTCTTGGTCTCCTTCCTGGTGGATGCCCGTGGAGGAGCAATGCGCGGCTGTCGGCACAGTGGCGTTCGCA *F TGATCATCCCTTCGAGGTCCACTTGTCAGCCAACCAGGGTAACCTGTCGTTATGTAAAGCCGCAGCGCACAATGCATCC *F GCCCCAGTTGATGGAGGGCGAGGCTTTGGCCAGTCGTGTCCTGGAACTGGGTCCTTGCTCCACCAAGTTTATTGGGCCA *F GTGGTCATGGAGGTGCCACACTTTGCATCGCTGCGTGGCAAGGAGCGCGAGATCATAATATTGCGATCGGACAATGGAG *F AAACATGGCGGGAGCACACCATCGATAACTCCGAGGAGATCATACACGATGTCCTGCAGCAGTGCTTTGAACCAGAGGA *F GATTGCCCAACTGGAGGAGCAGGCTGGCAACCATGTCTGCCGCTTTGTCACCTACGACTTCCCTCAGTACTTTGCCGTC *F GTATCGCGCATACGCCAGGAGGTCCATGCCATTGGACCGGAGGGCGGCATGGTGTCAAGCACCGTGGTGCCACAGGTGC *F AGGCCGTCTTCCCACAGGGAGCCCTCACCAAGAAGATCAAAGTTGGCTTACAGGCCCAACCGGTTGATCCCGATCTCAC *F GGCGAAGCTTTTGGGTCGCGGCGTGGCCGTATCCCCAATTGTTACGGTCGAGCCGCGTCGCCGTAAATTCCACAAGGCC *F ATCACCCTCAGCATGCCCGCCCCCAAGGCTCACGGCCAGGGTA! *F TGATTAACCAGTACTCGGGCAATACGCCCACCCTGCGTCTGCTATGCTCCATTACAGGC! *F GGACCATCTCGTGCTCAATGGGAAGATGTCACCGGTTCCACGCCCTTGACATTTGTCAATGACTGCGTCTCCTTTACGA *F CCACAGTATCAGCTCGTTTCTGGCTGATGGATTGCCGCAACATTTCGGATGCCACCAAAATGGCAACTGAACTGTACAA *F GGAAGTCATCCATGTGCCGTTTATTGCCAAGTTTGTGGTATTCGCCAAGAAGGTAGAACCATTCGAAGCAAGACTCCGA *F GTCTTTTGCATGACCGACGATCGTGAGGACAAAACTCTGGAGAAGCATGAGCTCTACACAGAAGTGGCCAAGAGCCGCG *F ATGTCGAGGTCCTCGAAGGCAAGCCGCAGTACATCGAGATGGCTGGCAATTTGGTGCCAGTGACCAAATCTGGCGACCA *F GCTCCAGGTGCAATTCAAGGCCTTCCGAGAGAACCGCCTACCGTTCACAGTACGAGTCAAGGATCAACATGCCGACATT *F GTTGGACGCACTTTGTTCATGAAGGAACCAAAGGTAGCCAAGGGCGAGCCACCGCAACAGCCCATCTGCATCCTGAACA *F TCGTTCTTCCGGAAGCTGTAATTCCCGATTCCACGACCGCCTTTTCGGACCGCGTCACCTCCGCCTACAGGACCTCAAT *F GTTCAGCTTGAGCAAGCATCAAAATGACCATTACATTGGCGACATCCGCATCGTCGATCTATCCAACCTGTTGGGTAAG *F GATTGGATTCAGCTGGCCCCGGAGATTGGCATCAATGGAGAGGAGATCGATGAGATCATCAACCAAAACACAGATAGCA *F TTGCCAGGCAGGCGCAGAGCATGATTCGCCTGTATAAGGACAAACCTAACTACGATATTCTTTCGCTGGAAACTGCTCT *F CAAAAACATTGGACGAGACGACATCATGAAAAAGTGCAAGAGTGGACGACTATCGCATTCGCGTGAATTCGATGAGGCG *F GACCTTATGAAGAACTCGGAGTCCGTGGAGGAACTGGTTCGCA! *F GGGAAAGCAAGCGAATCCAGCAAATTAACGAACGCGAAGAAGTCAAATACTCTGCCGAGGAGAAGGAGGTCGAGGAATC *F GGAGTCTGATGAAGAAGCTGCCAAGCGAACCGTAGCCGAACGGCGGGAAAAGATTGTTAAGCGTCTGTCCATCGAACGA *F TCAATTCCAGCCTCCACTCAGAAAAAAGAGATCACCCGCGAAATAACCGAAATCAAGCGTAAGAGTCTAATCGAGGACA *F AGAAGGCTCACCATGAGTCCGAAATTCTTATGCAACTGCCTGCGGATAACGTGATTATCAAGACAACAACTGTTCCCGA *F CCAAGTCATTAAGATGAAGATGGGCAAGATGGACTCAACCGAGGTGAGCAAGAGCGAGTTCGACAAGGAACTTACGCAC *F AAGTTCAAGACATCAGGGCGTAGCTCAGAGGAGGAAGACCAGCCATCGTCTCCAGATCAGACTGACAAGATTGTCCAGG *F ACATTAGTGCTGCCGAAAAGAAGGAGAAGGATGGCGTTACCTTCAGCCGAGTGACGACAATAACCCGCCAAGAGGCCCG *F CGACATTACCGAAGACTTCTTAGAGATTGAGAAGCGCAGCCAGCTCCCGGCGACTTCCACAACCGCAACAGTTCATGAG *F AAGTTCGTTGAGGAGATCAAGGAGAAGACCAGCCCTCTGGCTTCAGTGCCACAAGAAACAGTCAAGGAGGTGCAACAAG *F TCATCAGCGAGGTGACCGAAATAGCCAGCAAGAAGGTTGAAAACATTATTAGCTCCTTTGAGAGTAGCAAGTCTGTGGA *F TGCTACTACAGTTCTGCCCACACAGCCGTCTGTTGAATCAACGAAGGTTAGCGAGACCATTAAGAACCTCGAGGATGCC *F AAGGCCGTGTCTGCGGAACAAGTGAAAACTGTCCATGTCGTTGAGAGCTCTAGTATCGAGGAGACTATTGCCGAATTCG *F AAGCCAAGAAGGTTAAATATGACTTCCATGGCGGTGAGCCCAA! *F GACACAGATCCCAAAGTTTACGCGGAAACCAAGCGATGACTCTATGAAGCCCACCGCAG! *F CTCCACGTGCTACAGTTGAATCCGAAACTGAATCGGTCATTGAGACTAAGGCCGAAAAACCGATCTCAAAGATACCAGT *F AAAGACAATACCAACAGAAGCTCAGAAGGTTAGCGAAGTAGATGCACGTAAGATTACTCAAGACTTCCTCCAGGGTGAG *F AAACTGGCGGCCGAACCCAAGCCATCGCCAGCAGCCAGCAAGATACCAAAGGTGGAGCCGAGGAAATCTGTTGATAAGC *F AGGTCGATCGGGAATCAAAAATCTTGGATGATGTTGTGGCCTCTACGGCCACCATTATGACCGCCGGACTGGGAGATGA *F GCAGCTCAAGGATCAACTCGTCGATCACTCAGAAATTATCGCCAAGTCCGAAACAGTCGCTGAAAAGGTAACCGAACTG *F CTTGACACATTCCACAAGATCGAGGAGAAGGTCACCAAATCTAAAAAGACCTCGGAAATATCGAGCAAAGTGGAGGAAC *F TGGTTAAAATCGAGGAAAAACCTCTCTCACAAGTTCAACCCAAGGAGGACAAGGTGGCGGAAAAGGTTGCTGAAGTTAT *F CGAAACATTCCACAAAATCGAGGAGCAGATCACCACTGCCGATGCCCGCGAGCTAACTCGTGATTTCCTGAGCACGGAA *F CAACGAAACCAATTGCCGAGCATGCCACAAGCTGCTGAGAAGCCCCTTGAATCTAGCTTGACATCCACCAGTGCCTCGG *F AACCAGAATCAATAGTCACAGTGAAGCCATCACCCCCAGCGAGTAAGATACCTGTAGTGGAACCCAGGAAAATCGTTTT *F CGACGAATCTACAAAGCCTCTGATCGAGCCTGAGCCAGTGAAGACCACTGAAAAGAAGCCTCTTAATGAGCGACAACTA *F ACCGCTGATTTCTTGAGCATGGAGCAACAAACCCAGCTGGTCTCTGAGCCTGCCAAATCGTTGGTGGAAGAGGTGATGA *F AGTCCGCCGAACAAATGGTGGAGCAACCGAAACAGCAAAAATC! *F CCTGAATGAGCGACAACTTACAGAGGACTTTCTGCTTATGGAACAGCAGACACAACTGCATTCAGACATTGTTAAGCCA *F ACGGATAAGCTGATCGATGGTATTGAGTCCGCAGCGCCGGTTGGAGATGAAGCATCACCGTTCCACACGCCCAAGCTGA *F CCACCAGTGTGGTGACACAGGAGTCACAGCACCTGGCGAGTGAATATGACAGTGACACTTTCGGCAAACAGGCCACCAT *F ACCGTTAGGAGACTCAAAGATTGATCAGGGACTCACTGCACCAGTATCCATGGAACCACGAAAGTCACTGACCGATGCC *F GAGTTCTGCAAATCGGTGGGCGAAACAATCACCAAGAAGATGAGTGTGGGAGTTATTGAAATAAGTGATGAGCTCAAGA *F AAATTGAGAGCGAAATTCCACATTCCCAGACTCCTCCGCCAACACCGAGTGACAACAAAACCGACAAGCAGAACGAAGA *F GCCAGAACTGATCAGCTTGGAACGTGATTACCTGGAGAGCGAAATTCCACATTCCCAGACTCCTCCGCCAACACCGAGT *F GACAACAAAACCGACAAGCAGAACGAAGAGCCAGAACTGATCAGCTTGGAACGTGATTACCTGGCCGACACTGTGACCA *F CCCTTCACACAACTACAGAGTCGACCCTCGAACGCGTTTCTGCCATTACTAAAATTGTTTTGCCACATTTCGTAGTGAT *F CCTTGAAATATTTTTCAGTTTTATCCCATTTCGGTACTACTCTCCTCTGTTCCTCTACATTCCATCTTGA *F Protein sequence: *F > MVTENGAQGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKG *F NTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHD *F KVVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSA *F KHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQG *F EHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHG *F ASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTP *F LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVA *F QLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAE *F SKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASH *F FGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTI *F TKEDETAAAPSQAEEKYRVVAPEAMHESFMSDSEEEGGEDNMLSDQPYRYLTVDEMKSLGDDSLPIDVTRDERMDSNRM *F TQSAEYASGVPPTIGEEVISPHKTQVYGSSPKATVDGVYIANGSGHDEPPHVGRKLSWKSFLVSFLVDARGGAMRGCRH *F SGVRMIIPSRSTCQPTRVTCRYVKPQRTMHPPQLMEGEALASR! *F VLELGPCSTKFIGPVVMEVPHFASLRGKEREIIILRSDNGETWREHTIDNSEEIIHDVLQQCFEPEEIAQLEEQAGNHV *F CRFVTYDFPQYFAVVSRIRQEVHAIGPEGGMVSSTVVPQVQAVFPQGALTKKIKVGLQAQPVDPDLTAKLLGRGVAVSP *F IVTVEPRRRKFHKAITLSMPAPKAHGQGMINQYSGNTPTLRLLCSITGGPSRAQWEDVTGSTPLTFVNDCVSFTTTVSA *F RFWLMDCRNISDATKMATELYKEVIHVPFIAKFVVFAKKVEPFEARLRVFCMTDDREDKTLEKHELYTEVAKSRDVEVL *F EGKPQYIEMAGNLVPVTKSGDQLQVQFKAFRENRLPFTVRVKDQHADIVGRTLFMKEPKVAKGEPPQQPICILNIVLPE *F AVIPDSTTAFSDRVTSAYRTSMFSLSKHQNDHYIGDIRIVDLSNLLGKDWIQLAPEIGINGEEIDEIINQNTDSIARQA *F QSMIRLYKDKPNYDILSLETALKNIGRDDIMKKCKSGRLSHSREFDEADLMKNSESVEELVRRESKRIQQINEREEVKY *F SAEEKEVEESESDEEAAKRTVAERREKIVKRLSIERSIPASTQKKEITREITEIKRKSLIEDKKAHHESEILMQLPADN *F VIIKTTTVPDQVIKMKMGKMDSTEVSKSEFDKELTHKFKTSGRSSEEEDQPSSPDQTDKIVQDISAAEKKEKDGVTFSR *F VTTITRQEARDITEDFLEIEKRSQLPATSTTATVHEKFVEEIKEKTSPLASVPQETVKEVQQVISEVTEIASKKVENII *F SSFESSKSVDATTVLPTQPSVESTKVSETIKNLEDAKAVSAEQVKTVHVVESSSIEETIAEFEAKKVKYDFHGGEPKTQ *F IPKFTRKPSDDSMKPTAAPRATVESETESVIETKAEKPISKIPVKTIPTEAQKVSEVDARKITQDFLQGEKLAAEPKPS *F PAASKIPKVEPRKSVDKQVDRESKILDDVVASTATIMTAGLGD! *F EQLKDQLVDHSEIIAKSETVAEKVTELLDTFHKIEEKVTKSKKTSEISSKVEELVKIEE! *F KPLSQVQPKEDKVAEKVAEVIETFHKIEEQITTADARELTRDFLSTEQRNQLPSMPQAAEKPLESSLTSTSASEPESIV *F TVKPSPPASKIPVVEPRKIVFDESTKPLIEPEPVKTTEKKPLNERQLTADFLSMEQQTQLVSEPAKSLVEEVMKSAEQM *F VEQPKQQKSLNERQLTEDFLLMEQQTQLHSDIVKPTDKLIDGIESAAPVGDEASPFHTPKLTTSVVTQESQHLASEYDS *F DTFGKQATIPLGDSKIDQGLTAPVSMEPRKSLTDAEFCKSVGETITKKMSVGVIEISDELKKIESEIPHSQTPPPTPSD *F NKTDKQNEEPELISLERDYLESEIPHSQTPPPTPSDNKTDKQNEEPELISLERDYLADTVTTLHTTTESTLERVSAITK *F IVLPHFVVILEIFFSFIPFRYYSPLFLYIPS *F Comments: Here I am sending you the Ank2 (CG7462) long e transcript with its *F ORF. *F Read my explanation in the previous message. *F Browser: Mozilla/4.73 (Macintosh; U; PPC) *F Accessed from: query.pl, /www/hedgehog_8002/htdocs # *U FBrf0146859 *a Howard *b K. *t 2002.5.8 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Wed May 08 21:31:10 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F To: flybase-help@morgan.harvard.edu *F Subject: PAP2G *F comments: This name was given by Anne Hayden to what is now called wunen-2, *F and which Anne and MY sequence submission refers to as tunen ( *F mailto: flybase-help@morgan.harvard.edu *F realname: ken howard *F reply-to: ken.howard@ucl.ac.uk *F Sent from computer inktomi5-bre.server.ntl.com # *U FBrf0148887 *a Lehmann *b M. *t 2002.6.12 *T personal communication to FlyBase *u *F From mlehmann@howard.genetics.utah.edu Wed Jun 12 21:17:11 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 12 Jun 2002 21:17:11 +0100 *F Date: Wed, 12 Jun 2002 16:12:46 -0700 *F From: Michael Lehmann *F X-Mailer: Mozilla 4.7C-CCK-MCD {C-UDP; EBM-APPLE} (Macintosh; I; PPC) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F Subject: Pipsqueak family *F Content-Type: multipart/mixed; boundary='------------EEA638BA9476FE8AE8C9D94A' *F Content-Length: 2584818 *F *F Dear Flybase curator, *F *F I would like to draw your attention to a few recent publications that *F you may want to consult in updating the entries for several Drosophila *F genes. Proteins encoded by the pipsqueak (psq) gene contain an unusual, *F experimentally identified DNA-binding domain, which consists of 4 *F repeats of a 50-amino acid motif (Lehmann et al., 1998: J. Biol. Chem. *F 273, 28504--28509). Subsequent database searches confirmed that this *F motif defines a novel family of eukaryotic helix-turn-helix (HTH) *F DNA-binding proteins (Siegmund and Lehmann, 2002: Dev. Genes Evol. 212, *F 152--157). This family includes proteins encoded by 14 Drosophila genes, *F which are listed below. The presence of Psq DNA-binding motifs has also *F been noticed by Shim et al. (2001: Development 128, 4923--4933) in the *F Ribbon protein and by Couderc et al. (2002: Development 129, 2419--2433) *F in the Bric à brac proteins 1 and 2. Based on its first identification *F in the Psq protein, the authors of all the above-mentioned papers *F consistently refer to the new type of HTH domain as a “Psq DNA-binding *F domain” and to the defining 50-amino acid motif as the “Psq motif”. *F Drosophila genes that encode proteins with a Psq DNA-binding domain are: *F *F pipsqueak (psq) *F Tyrosine kinase-related protein (Tkr) *F ribbon (rib) *F bric à brac 1 (bab1) *F bric à brac 2 (bab2) *F piefke (pfk) *F BTB-protein-VII (Btb VII) *F Eip93F (E93) *F CG3726 *F CG8924 *F CG13651 *F CG11849 *F CG13496 *F *F A Psq DNA-binding domain is also present in the transposase encoded by *F the transposable element pogo. *F *F For your convenience, PDF files of the papers cited here are attached to *F this message. *F *F Sincerely, Michael Lehmann *F ------------------- *F Michael Lehmann, Ph.D. *F Howard Hughes Medical Institute *F University of Utah *F 15 N 2030 E, Room 2100 *F Salt Lake City, UT 84112-5331, USA *F phone: 001-801-581-2612 *F fax: 001-801-581-5374 *F email: mlehmann@howard.genetics.utah.edu # *U FBrf0149565 *a Smith *b D. *t 2002.1.14 *T personal communication to FlyBase *u *F FASTA sequences of Obp (Odorant-binding protein) genes described in *F FBrf0141547 (Galindo and Smith, 2001, Genetics 159(3): 1059--1072). *F Dean Smith, M.D., Ph.D. *F Department of Pharmacology and *F Center for Basic Neuroscience *F University of Texas Southwestern Medical Center *F 5323 Harry Hines Blvd. *F Dallas TX. 75390-9111 *F 214/648-1650 *F fax 214/648-1801 *F smith15@swvx12.swmed.edu *F \------------------------------------------------------------------------------ *F -- *F >18a *F MKVVCSIAVLWICLITMVSSWQSAGRVNAEGCLKHHNLTSAQVQAVAPSTPVADVPVAVKCYSRCLIQDYFGDDGKIDL *F QKVGKRGSQEDHVILSQCKQQFDGVTNLDTCDYPYLILQCYFKGKQSGTIAS *F >19a *F MKFHLLLVCVAISLGPIPQSEAGVTEEQMWSAGKLMRDVCLPKYPKVSVEVADNIRNGDIPNSKDTNCYINCILEMMQA *F LESTLKQMDIMLPDSYKDEYRKGINLCKDSTVGLKNAPNCDPAHALLSCLKNNIKVFVFP *F >19b *F MTTTLKMTNLLLAVACAAVLMGSATADEEEGSMTVDEVVELIEPFGDACTPKPSRENIVEMVLNKEDAKHETKCFRHCM *F LEQFELMPEDQLQYNEDKTVDMINMMFPDREDDGRRIVKTCNEELKAEQDKCEAAHGIAMCMLREMRSSGFKIPEIKE *F >19c *F MKPSTPVAAIPLMTIVVAVLLQTHCVRGQTQAFDLAKLLPKTGTEPIWAVIDRNLPQVQELVTAARMECIQKLQLPRDQ *F RPLGKVTNPSEKEKCLVECVLKKIKLMDADNKLNVGQVEKLTSLVTQDNKMAIAVSSSMAQACSRGISSKNPCEVAHLF *F NQCISRQLERNNVKLVW *F >19d *F MSHLVHLTVLLLVGILCLGATSAKPHEEINRDHLLELANECKAETGATDEDVEQLMSHDLPERHEAKCLRACVMKKLQI *F MDESGKLNKEHAIELVKVMSKHDAEKEDAPAEVVAKCEAIETPEDHCDAAFAYEECIYEQMREHGLELEEH *F >22a *F MRVLLAFVLLLGLSVLATKEPEEVKIVSECAKENNVHRKKALDLLMSYRLKKKTHNVMCFINCIFERTNILQKVKEKVV *F KENHNCDSIKDADKCAESFQKFQCLVKIEMKRDSAATRHVPQTMPKL *F >28a *F MQSTPIILVAIVLLGAALVRAFDEKEALAKLMESAESCMPEVGATDADLQEMVKKQPASTYAGKCLRACVMKNIGILDA *F NGKLDTEAGHEKAKQYTGNDPAKLKIALDIGETCAAITVPDDHCEAAEAYGTCFRGEAKKHGLL *F >47a *F MNRVLVLLLVLKMFALSEININLGLTVADESPKTITEEMIRLCGDQTDISLRELNKLQREDFSDPSESVQCFTHCLYEQ *F MGLMHDGVFVERDLFGLLSDVSNTDYWPERQCHAIRGNNKCETAYRIHQCQQQLKQQQQNLLATKEVEVTTTPAGSDET *F KP *F >51a *F MKVFIGLVLLLAVTTLSSALFESEANECAKKLGITPDYFENFPHSSRVKCFYHCQMEKLEIIANGVVTPFDLKVLNISP *F ESYDKYGVKVKPCLKLSHRDKCELGYLVFQCLKREFNL *F >56a *F MNSYFVIALSALFVTLAVGSSLNLSDEQKDLAKQHREQCAEEVKLTEEEKAKVNAKDFNNPTENIKCFANCFFEKVGTL *F KDGELQESVVLEKLGALIGEEKTKAALEKCRTIKGENKCDTASKLYDCFESFKPAPEAKA *F >56b *F MKLIYLLVVFLIFALSELVAGQSAAELAAYKQIQQACIKELNIAASDANLLTTDKEVANPSESVKCYHSCVYKKLGLLG *F DDGKPNTDKIVKLAQIRFSSLPVDKLKSLLTSCGTTKSAATCDFVYNYEKCVVKGISA *F >56c *F MFFIFYISFVNSTYVVLGIICFIFTSQEMLRACMPPTPVQSIGDVNNLGDLDFNGNSQMPYLDLKHNEPLQCFVSCLYE *F TLDLDRYNVLLEEAFKNQVQTIIQHEKAEIKECSDLQGKTRCEAAYKLHLCYNHLKTLEAEQRIREILERTEAENEGFG *F PEGSDFIDGIQHSGEAMTTAKSE *F >56d *F MKFLIVLSVILAISAAKAVAHANGALCAQQEGITKDQAIALRNGNFDDSDPKVKCFANCFLEKIGFLINGEVQPDVVLA *F KLGPLAGEDAVKAVQAKCDATKGADKCDTAYQLFECYYKNRAHI *F >56e *F MKVFFVFAALAALSLASAVGLTDSQKAEAKQRAKACVKQEGITKEQAIALRSGNFADSDPKVKCFANCFLEQTGLVANG *F QIKPDVVLAKLGPIAGEANVKEVQAKCDSTKGADKCDTSYLLYKCYYENHAQF *F >56f *F MKVFLLFIFISAIWLQAFCMKSSEKIKACLKRQLGYTITENTKFDAKEDSLQSKCFYHCLLEVKGVIANDAISSEQPRK *F VLEKKYGITDTDELEKAEEKCHSIKASGKCELGYEILKCYQSITKH *F >56g *F MRATFALTLLLGCLSGILAQQANIDSSVSKELVTDCLKENGVTPQDLADLQSGKVKAEDAKDNVKCSSQCILVKSGFMD *F STGKLLTDKIKSYYANSNFKDVIEKDLDRCSAVKGANACDTAFKILSCFQAAN *F >56h *F MKFTLFCIALAAFLSMGQCNPDFRQIMQQCMETNQVTEADLKEFMASGMQSSAKENLKCYTKCLMEKQGHLTNGQFNAQ *F AMLDTLKNVPQIKDKMDEISSGVNACKDIKGTNDCDTAFKVTMCLKEHKAIPGHH *F >56I *F MHFFTCCALLLVVVTLPTCFVQAGPIKDQCMAAAGITAQDVANRHETDDPGHSVKCFFRCFLENIGIIADNQIIPGAFD *F RVLGHIVTAEAVERMEATCNMIKSETSHDESCEFAWQISECYEGVRLSDVKKGQRTRNHRG *F >57a *F MFNTRLAIFLLLIVVSLSQAKESQPFDFFEGTYDDFIDCLRINNITIEEYEKFDDTDNLDNVLKENVELKHKCNIKCQL *F EREPTKWLNARGEVDLKSMKATSETAVSISKCMEKAPQETCAYVYKLVICAFKSGHSVIKFDSYEQIQEETAGLIAEQQ *F ADLFDYDTIDL *F >57b *F MFIYRLVFIAPLILLLFSLAKARHPFDIFHWNWQDFQECLQVNNITIGEYEKYARHETLDYLLNEKVDLRYKCNIKCQL *F ERDSTKWLNAQGRMDLDLMNTTDKASKSITKCMEKAPEELCAYSFRLVMCAFKAGHPVIDSE *F >57c *F MLKLWLICILTVSVVSIQSLSLLEETNYVSDCLASNNISQAEFQELIDRNSSEEDDLENTDRRYKCFIHCLAEKGNLLD *F TNGYLDVDKIDQIEPVSDELREILYDCKKIYDEEEDHCEYAFKMVTCLTESFEQSDEVTEAGKNTNKLNE *F >57d *F MPEKMSLRLVPHLACIIFILEIQFRIADSNDPCPHNQGIDEDIAESILGDWPANVDLTSVKRSHKCYVTCILQYYNIVT *F ASGEIFLDKYYDTGVIDELAVAPKINRCRYEFRMETDYCSRIFAIFNCLRQEILTKS *F >57e *F MLDQLTLCLLLNFLCANVLANTSVFNPCVSQNELSEYEAHQVMENWPVPPIDRAYKCFLTCVLLDLGLIDERGNVQIDK *F YMKSGVVDWQWVAIELVTCRIEFSDERDLCELSYGIFNCFKDVKLAAEKYVSISNAK *F >69a *F MVARHFSFFLALLILYDLIPSNQGVEINPTIIKQVRKLRMRCLNQTGASVDVIDKSVKNRILPTDPEIKCFLYCMFDMF *F GLIDSQNIMHLEALLEVLPEEIYKTINGLVSSCGTQKGKDGCDTAYETVKCYIAVNGKFIWEEIIVLLG *F >76c (LUSH) *F MKHWKRRSSAVFAIVLQVLVLLLPDPAVAMTMEQFLTSLDMIRSGCAPKFKLKTEDLDRLRVGDFNFPPSQDLMCYTKC *F VSLMAGTVNKKGEFNAPKALAQLPHLVPPEMMEMSRKSVEACRDTHKQFKESCERVYQTAKCFSENADGQFMWP *F >83a *F MALNGFGRRVSASVLLIALSLLSGALILPPAAAQRDENYPPPGILKMAKPFHDACVEKTGVTEAAIKEFSDGEIHEDEK *F LKCYMNCFFHEIEVVDDNGDVHLEKLFATVPLSMRDKLMEMSKGCVHPEGDTLCHKAWWFHQCWKKADPKHYFLP *F >83b *F MLKYPLILLLIGCAAAQEPRRDGEWPPPAILKLGKHFHDICAPKTGVTDEAIKEFSDGQIHEDEALKCYMNCLFHEFEV *F VDDNGDVHMEKVLNAIPGEKLRNIMMEASKGCIHPEGDTLCHKAWWFHQCWKKADPVHYFLV *F >83c *F MQMKSGILIALCLCLSLNEGLALLEHEGETINRCIQNYGGLTAENAERLERFKEWSDSYEEIPCFTRCYLSEMFDFYNN *F LTGFNKDGIVGVFGRPVYEACRKKLELPFESGESSCKHAYEGFHCITNVSRHLNFTDLHR *F >83d *F MRIYTTLLNILQMESHPFTVIDNMPNISPSAKDAMKDCLQDVHQDEWKSFDAFAYYPVNEPIPCFTRCFVDKLHIFEEK *F TRLWKLEAMKQNLGIPAKGARIRTCHRHRGRDRCATYYKQFTCYAMAV *F >Pseudogene 83e *F LSTLLQCSRQLNATNVELLQYSKLKSKEPIPCLFQCFADAMGFYDPDGNWRLENWKQAFGPSGNEDQSSGADYSGCRL *F SGTQREVALSKCSWMYHEYKCWERVNGNKLVEDNEEQ *F >83f *F MSSPRAVLVSLFLICSQALADLSGDAQTLEKCLRQLSSPESIAGDLRKLERYSSWTREEVPCLMRCLAREKGWFDVEEN *F KWRLKQLTEDLGADVYNYCRFELRRMGSDGCSFAYRGLRCLKQAEMHAGTS *F >83g *F MQSQSLLLIVAAVATFLVAQTTAKFLLKDHADAEKAFEECREDYYVPDDIYEKYLNYEFPAHRRTSCFVKCFLEKLELF *F SEKKGFDERAMIAQFTSKSSKDLSTVQHGLEKCIDHNEAESDVCTWANRVFSCWLPINRHVVRKVFA *F >84a *F MYSALVRACAVIAFLILSPNCARALQDHAKDNGDIFIINYDSFDGDVDDISTTTSAPREADYVDFDEVNRNCNASFITS *F MTNVLQFNNTGDLPDDKDKVTSMCYFHCFFEKSGLMTDYKLNTDLVRKYVWPATGDSVEACEAEGKDETNACMRGYAIV *F KCVFTRALTDARNKPTV *F >99a *F MKVFVAICVLIGLACADYVVKNRHDMLAYRDECVKELAVPVDLVEKYQKWEYPNDAKTQCYIKCVFTKWGLFDVQSGFN *F VENIHQQLVGNHADHNEAFHASLAACVDKNEQGSNACEWAYRGATCLLKENLAQIQKSLAPKA* *F >99b *F MKVLIVLLLGLAFVLADHHHHHHDYVVKTHEDLTNYRTQCVEKVHASEELVEKYKKWQYPDDAVTHCYLECIFQKFGFY *F DTEHGFDVHKIHIQLAGPGVEVHESDEVHQKIAHCAETHSKEGDSCSKAYHAGMCFMNSNLQLVQHSVKV* *F \------------------------------------------------------------------------------ *F -- # *U FBrf0149570 *a Faivre-Sarrailh *b C. *t 2002.3.7 *T personal communication to FlyBase *u FlyBase error report for CG1084 on Thu Mar 7 06:07:13 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 7 Mar 2002 06:07:13 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: sarrailh@lgpd.univ-mrs.fr *F Subject: FlyBase error report for CG1084 on Thu Mar 7 06:07:13 2002 *F Error report from Catherine Faivre-Sarrailh (sarrailh@lgpd.univ-mrs.fr) *F Gene or accession: CG1084 *F Release: 2 *F Gene annotation error *F Gene CG1084 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CTTCTGGACTGTTTTGTTTTTATTTAATTAAACTATAATTTAAAAAGCTG *F TAAATTGCCTCTGATACTTATTTTGTCCGGATCCGCACAACTCGTGGTTT *F TTTTTTTTGTCAGCAGGAACACCTCGCAGTGGTAGCGTTTTCAACCGTTC *F GTGTGCTGCGTCCTGGAGTCGTAATTAAGAAACTATCGAATTCCAGAGCA *F AAATGTGCCTTTAGTCTGCTGCGAGACATCTAAACCAGAACCCACGCTAT *F TAAAGCTAAGGACCAACTATGCTAGCAAAAATCGGCCTTCTGGCGAGTAT *F TCTTGTGCTCAACTTAGTTGGCCAGATCACGCCCCAATTTTCCGAAAACC *F TGCCGGATCCGGATCCGCAGTCGGGACAGCAGCCTCAGAACTATCAGCCT *F AGCTACAACAAGGATTACTCTCCGCGATACAACCCCCTGTATACTGGACA *F GCAAAGCGCTGACCCCAACCAGTTCGACAATACGCTGGTCGACGGACAGA *F GCCCTAATACCTACAAGGGGTACTATGATGGCAGGGCAGGAGGTGGGGGC *F CTTGGCGGCAATGTAGTCGGGCCTGGAAACAACCTCGGCGGACTCGGCCC *F ACAGTACGACCCCTTTAATCGGAACAGCATAGGTTCAGCAGGTGTCAGCT *F ACCGGGACGCCTATACGGATGAAGATAACTTTTGCCCGGAGCACTGGGTC *F TCGTTCAGACAGACGTGCTATCGCTTCATCCGCTCGCCCAAGCGCAACTG *F GGCAGAGGCTAAAAAGATCTGCAAGGCACACAATGCCGACCTGATAAACG *F TGGATAATGTAGAGAAGCACTCGTTTATACTCAAGAATCTCATCCTGCAG *F AATCAACGGCAGAACCGATTCTTCATCTCCGCCCGGCAAACGGGCCCGTT *F GAATTGGGTCAACGATGACAACACCCAGTTAGTCCAGATCGAAGACTCCT *F TTTCCATGGACGAGCAAGTGCCCTTGGAAAACGAAGATCTGCACGACAATAGATTCCTTGTGCAAAACGACCTCAACAA *F CCAAAATATCAATAATCCAAATCAGTTTTACAACTCTCTTCCGGGGACTGTTAATCAGCGCAATCAAAATAACCTTCGC *F GGCTTTATAGGTCCCAACCAGCCGTATGGAGACAACCGATATGTGCGCGATCGCGTTGTGTACGCCTTCTCAAAGAAAA *F GGGATCGGTGGATGTTCATGCCAGC *F CTATGAAATTGAACTAAATCTCTTTATATGCGAGTCAAAAGTACTATACA *F GCTCAGACAATGTCAACATAAAGTTAGACGACAAGCGTCCCTACCATTAT *F GGACTCGATATAAACGACATGGAACGGATTCCACGGGGTCCTTATTTCGT *F AAAGCAGCCAAACGACACGACTTTCGATGTGAACAAGAATCGCCTGATTA *F ATGATGTTACTCTTAGCTGTTTGGCCAACGGGTATCCAACACCTTCATAC *F ACATGGTACCGAGAGGTATACGTAGACGACAGACTCGAGTACCAAAAGAT *F TGATCCCCTGGCGCAGGATCGATACACCATATCAGGAGGCAACCTGATTA *F TCTACGAGCCGAAGCAAGCGCTGGACCAGGGCGCGTATCATTGTGTGGCA *F GAAAACAAATTTGGGCGAATTCGATCCGAAAGCGCGCATTTAAACTTCGG *F TTTCATTATGGAATTCAATCTAAAACGGTCTGCGGAGACAAGCGAGATGA *F ATTGGGGCAAATCCATATTCTGTGACCCCCCGCAGCACTATCCCGACGTA *F CGCTATTACTGGGCTCGAGACTATTTTCCTAACTTCGTGGAGGAAGATCA *F ACGCGTATTCGTCTCCCGCGATGGTGCCCTATACTTCTCCTTCATAGAAA *F CTGTGGACAGAGCCAACTACTCGTGCACCGTACAGACCCTTGTGTCGGAC *F ACCGGTCGCAACGGACCCTTCTTTCCGTTGCGCGTTACCCCCAACAGCAA *F CTATCAAGCACTGATATTTGCCAACACCTTCCCTAAAGTCTTTCCGGAGG *F CGCCAGTGGCAGGTGACGAGATTCGGCTGGAGTGCATGGCCTTCGGTTAC *F CCCATTCCGTCATACAATTGGACCCGCCAAGGCCTACCTCTCCAACGCAA *F CGCCTACACGATCAACTATGGGCGTGTCTTAATAATCCAAAATGCGACAA *F CGAACGACAACGGCGAGTACTCCTGCACGATTACCAATCCCCGGAAGACA *F CTTATGAAGAGCATATACATCAATATTCAAATGCGCCCACAATTTACCAT *F ACCTCTTAAAGACATGATAAAAGACTACAACAGTGACGTGACTTTCATTT *F GCGAGGCATTTGCAATTCCGGACGCGAATTACACGTGGTACAAAAATGCC *F GAACGACTCGACCCAGCGAATATTAATCGCGACCGATACATTATCCAAGA *F CAATGTGTTAACCATTAAATTTCTAGAAAAGGATAAAGACGACGCAATGT *F ACCAATGTGGTGCCCAAAACCAACTGAAGACCTCCTTCTCTTCGGCGCAG *F CTCCGAGTGCTGTCTATGAAACCGTCCTTTAAAAAGCATCCCCTTGAATC *F TGAAGTCTATGCTGTTTACAACGGCAATACGACAATTGTGTGCGACCCGG *F AAGCCGCTCCACGCCCTAAGTTTCAATGGAAGAAGGATGGACAGGTGATT *F GGCTCGGGAGGCCACCGGCGCATTCTGCCCAGTGGTACCCTGACAATATC *F ACCCACGTCGCGGGACGATGAGGGCATTTACACATGTATTGCATCAAACC *F AAGCCGGCACCGACGAATCGCACGCGCGTGTTATTGTATTGCAGGAAATT *F CGTTTCATTGAAACGCCTCCACAACGAATCGTTTCTAAGGAACACGACTT *F AATCTTCTTGCATTGCGAGGCCGCCTTTGATGAACTCCTCGACATTGCTT *F ATGTTTGGAAACACAACGGCGAAGTCCTAAAAAACAATCACGACGGCACC *F GGACGCATTATTGTAGATTGGAACAGATTGACAGTGCATAACACGAGCAT *F GCGCGATGCCGGGGACTACGAATGCGTTGTCAAGTCGGCGGTAAACGAAA *F TTAGCAGCAAGACCAGTGTTTCTATTGAGGGAGCTCCTGGCGCTCCTGGT *F GGTGTTCAAGTCATACAGATAAGTAAAACGAAGGCCATCATTGAGTGGGT *F GGACGGATCGCACAACGGTCGTGCGATCCGCTACTACAACATCCTAGGCC *F GTACAAACTGGAATCGCACCTGGGTTAATGTGTCGACCCATGTGCAGGCG *F CGAGAAGTAGATCGATACACCTCTCGCCAGCAAGCCGAGGTTGTCAATCT *F GACGCCGTGGTCGGCTTACGAATTTAGTGTTACTGCTGTAAACGATCTGG *F GAATCGGAACGCCCTCGGCCCCCTCGCCAATTTACAGCACCTATGAGGAC *F AAACCATACATAGCCCCGAGGAACGTGGGTGGTGGAGGTGGAAAGATTGG *F CGACCTAACTATCACCTGGGATCCGCTTCTACCGCAAGAGCAGCATAGCC *F ACGGCATACACTATAAAGTGTTTTGGAAGCTCAAGGGAGCCATTGAATGG *F GCATCTGACGAAATAAAAAAACAGGATCACATGGGCGTGGCTGTGGTTAA *F TATTCCTCTTAATAACTACTATACGGAGTACGAAGTCAAAGTCCAAGCCA *F TTAACAGTGTTGGCAAGGGACCGGAGAGTGAAATAGCTGTCATACACTCC *F GCTGAGGACATGCCACAGGTGGCTCCTCAAAAGCCCATTGCGCTCGCCTA *F CAACTCCACATGCTTTAATGTCACTTGGCAACCTATAGACATGTCCCGTG *F AAAACATTCGTGGCAAACTCATTGGCCACAGGTTGAAGTATTGGAAAACG *F ACGCACCAGGAAGAAGACTCGGTATACTACCTGTCACGTACTACAAGGAA *F TTGGGCGCTTATCGTAGGTCTACAACCAGATACTTACTACTTTGTTAAAG *F TCATGGCTTACAATGCTGCAGGTGAAGGACCTGAAAGCGAACGCTTTGAA *F GAACGAACATATAGAAAGGCCCCGCAAAAGCCGCCATCTTCGGTTCACGT *F GTATGGAATAAACCCGTCTACCGTGCGGGTTGTTTGGAGATATGTTTCAC *F CTTCGCAAGACGAGGAACCAGTAGAGGGTTATAAGGTTCGCATTTGGGAA *F TCGGACCAGAACATGATTACGGCCAACAATACCATAGTGCCAATCGGACA *F GAAACTGGAGTCGTACATCAACAATCTGACGCCAGGGAAGAGTTACAACA *F TGCGAGTCCTCGCATACAGCAATGGAGGAGACGGTCGCATGTCCAGTCCA *F ACTTTACACTTCCAGATGGGCAAGACCACGCGCAACGGCGCAAATACCCG *F ACATGGCCACAATATCAATACGGCTCTTATCTTAAGCACATTGCTACTCA *F TTAGCACCTTTCTTTACACAAGCCAATGA *F Protein sequence: *F >MLAKIGLLASILVLNLVGQITPQFSENLPDPDPQSGQQPQNYQPSYNKDY *F SPRYNPLYTGQQSADPNQFDNTLVDGQSPNTYKGYYDGRAGGGGLGGNVV *F GPGNNLGGLGPQYDPFNRNSIGSAGVSYRDAYTDEDNFCPEHWVSFRQTC *F YRFIRSPKRNWAEAKKICKAHNADLINVDNVERHSFILKNLILQNQRQNR *F FFISARQTGPLNWVNDDNTQLVQIEDSFSMDEQVPLENEDLHDNRFLVQNDLNNQNINNPNQFYNSLPGTVNQRNQNNL *F RGFIGPNQPYGDNRYVRDRVVYAFPKKRDRWMFMPAYEIELNLFICESKVLYSSDNVNIKLDDKRPYHYGLDINDMERI *F PRGPYFVKQPNDTTFDVNKNRLINDVTLSCLANGYPTPSYTWYREVYVDDRLEYQKIDPLAQDRYTISGGNLIIYEPKQ *F ALDQGAYHCVAENKFGRIRSESAHLNFGFIMEFNLKRSAETSEMNWGKSIFCDPPQHYPDVRYYWARDYFPNFVEEDQR *F VFVSRDGALYFSFIETVDRANYSCTVQTLVSDTGRNGPFFPLRVTPNSNYQALIFANTFPKVFPEAPVAGDEIRLECMA *F FGYPIPSYNWTRQGLPLQRNAYTINYGRVLIIQNATTNDNGEYSCTITNPRKTLMKSIYINIQMRPQFTIPLKDMIKDY *F NSDVTFICEAFAIPDANYTWYKNAERLDPANINRDRYIIQDNVLTIKFLEKDKDDAMYQCGAQNQLKTSFSSAQLRVLS *F MKPSFKKHPLESEVYAVYNGNTTIVCDPEAAPRPKFQWKKDGQVIGSGGHRRILPSGTLTISPTSRDDEGIYTCIASNQ *F AGTDESHARVIVLQEIRFIETPPQRIVSKEHDLIFLHCEAAFDELLDIAYVWKHNGEVLKNNHDGTGRIIVDWNRLTVH *F NTSMRDAGDYECVVKSAVNEISSKTSVSIEGAPGAPGGVQVIQISKTKAIIEWVDGSHNGRAIRYYNILGRTNWNRTWV *F NVSTHVQAREVDRYTSRQQAEVVNLTPWSAYEFSVTAVNDLGIGTPSAPSPIYSTYEDKPYIAPRNVGGGGGKIGDLTI *F TWDPLLPQEQHSHGIHYKVFWKLKGAIEWASDEIKKQDHMGVAVVNIPLNNYYTEYEVKVQAINSVGKGPESEIAVIHS *F AEDMPQVAPQKPIALAYNSTCFNVTWQPIDMSRENIRGKLI! *F GHRLKYWKTTHQEEDSVYYLSRTTRNWALIVGLQPDTYYFVKVMAYNAAGEGPESERFEERTYRKAPQKPPSSVHVYGI *F NPSTVRVVWRYVSPSQDEEPVEGYKVRIWESDQNMITANNTIVPIGQKLESYINNLTPGKSYNMRVLAYSNGGDGRMSS *F PTLHFQMGKTTRNGANTRHGHNINTALILSTLLLISTFLYTSQ *F Comments: The exon 1 is 162 bp longer than the predicted cDNA # *U FBrf0149571 *a Bouche *b N. *t 2002.4.2 *T personal communication to FlyBase *u FlyBase error report for CG8809 on Tue Apr 2 07:35:58 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 2 Apr 2002 07:35:58 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: n.bouche@leeds.ac.uk *F Subject: FlyBase error report for CG8809 on Tue Apr 2 07:35:58 2002 *F Error report from Nicolas Bouché (n.bouche@leeds.ac.uk) *F Gene or accession: CG8809 *F Release: 2 *F Gene annotation error *F Gene CG8809 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >DmCAMTA *F ATGGCCGTGATTGCGCCAAGTATCACACTTTGGGGCGACAAAAAGGAGTG *F GACCAAGGAGGAGCTGGTCAGCCAGCTCAAGCCCATGCTATCCACAATTA *F ATTCTGATGATGACTCCGACTCGGGTAACGATATAGAAATATCAACGGCG *F GAGACGGTGGAGTCCATAGTTTGCCAACTGATGGAGAAGCAGAGGTTGTC *F GCGTCAGGCTGCCTTGGTCAAGCAGCTGGACTGCGGGTGCGGAGATGGAG *F GATGCGTGGACGGAAAGACCTGTACGCATCCCGTGATGCGACGATCGGCC *F GCCATGCTGAAGTCCTCGGTGCAGGAGAAACGGCTGGGCGAGCCCTTCAA *F CGGCAACACGCCCAATGTGGTGGTGGCCAGCAAGCTCTACTCCCGATGGG *F CGGAGCGGCCAAGGCAGCACGTGGATAATCACGGCCAGTTCCACAACGTG *F ACGCAGCAGTTGCCGCAGGAGTCAGCCATTAAGTTTCAGATCATTCCGCC *F GCAGCAACAGCAGCAGCAGGATGGGAACTACCAGCAGCAGCAGTATCGGG *F GTGGAAGTCAAATGCTCGCTGCGAGGAGTAATCTGGTGATGCAGCAGCAG *F CAGCAACACCAGCAGCAGCAGCAATATCACCAGCAACAGCAACAGCAACA *F GCAACAGCAGCAGCAGCACCAACAACAGCAGCAGCATTTGAGTCTACAGC *F GATTTGTTGCCAGTCAAGGCCAGAATTCGGTGCATCTCAATCAGCAGCAC *F AGGCTGCAAATAGCCAATACAACGATCACAGCCACAGCCAGTGATCCTGC *F GACGACTTTAGCAGCAGCAGCAGCAGTAGCAGCAGCTTCAGGTGCAACAC *F CAGTCGCCTTGAATATAATGGACACCGAACTAATTGAAAACCAAAACCAC *F ATGACCGCAAACAAAATCACAAACTCCGGCAGCAGCAATAGCAGTAGCAG *F CAATGCCAGCAAGCAATTAGCAGGCCAAACAAACAACAAATTAAATGTCG *F TAAATAATAACGACCCCGGCAACAATAACTTTATGTACAATCAACAGCAG *F CAACTTAATGCTTCCAGCAACAGCAACAACAGCAGCCAGCAGCAGCAGCA *F ACAGCAGCAGCAACATTATTATAAATTGCAACAGACAACAGCAACCCCAA *F CTAGTGGTCAGCCGCCTCCTCTGGCGCAAGTTCAACCCCATTCCTTGGCC *F CAACCACCCGTGGAGGCCATGTGCATGTCACCCGAGCATCGCAGCAGCAG *F CCAGCCCACACCTGCAACGTCTTCCGCTGGTAGCATCCCCTCCTCCGTAT *F CCGTATCCATATCCGTGTCCACATCCACACCCACACCCGCTCCCACGTCC *F ATATCCACGCCCACATCGGGCACTTCGTCCACTTCGAGTTCCTTACAATC *F GATTATCGATGGCAATCAGCAGCAACAAATTACAACGACGTCGACGGCAG *F CAACTTGTGATAATTTAATGAGCGCCAATGCGCTCTCTGAGGATAGCCAC *F ACAGTCAACAATCAGGCCACAGAAGCCCCCAACAGGAGTCAGCTGCAATC *F GCAGTCGCAATCGCTATCCCTGAACCAAAATGGTTGTGCAACTTACAGTG *F CTTCCAGTGATAACAGCAGCCAAATTAGTGACGAAAGCAGCAGCTTTGGC *F GGTAACAATCAAAACAGCAGCAACAGCAGCACCAGCGAGGAGGAGCCCCA *F GGTGGAAGCGTTGAGCTTCTTCAACGAGACCCTGGATCTGTCCCAAGAGG *F ATATCCAGCGCACTCTGATAGCAAATATGCCGTATAATACGACAGCAGCG *F GGAGCCACAGCACCCAGTACAACGATAACAACTGGCAACACCAAACTCGA *F ATTAAGCCAACAGGAGACGAAGGAGAAACCTGCGATGGGAACAGAAACAG *F CAACCGAGATTGAGGATGATGAAACGGACGATGTGTTTGCCAATCTGGAT *F GCCTTCGATATGCTCGTGGAGTTTCCCGAACTGGACTTGGATGACAAGCA *F GGCCCTGAATAACACGGCTTTGGAGCAGAGCTCCTTTTTGGGAGAGTCCG *F CGCCATCGCAGCCACGCAAGGTGCACAATATATGCGACTTCAGTCCCGAA *F TGGTCGTACACGGAGGGAGGCGTTAAGGTTCTGGTTGCCGGACCTTGGAC *F GAGCTCGAATGGCGGTGCGTATACGGTGCTATTCGATGCGCAACCAGTAC *F CCACGCAACTGGTCCAGGAAGGAGTACTCCGCTGCTATTGTCCAGCCCAC *F GAGGCCGGATTTGTGACTCTGCAGGTGGCTTGTGGCGGATTCCTTGTTTC *F CAACTCCGTGATGTTTGAATACAAGCTCTCCCTGCTGGCGGATGCTCCAT *F TCGATGCCACCAGCTCCAACGATTGCCTGTACAAGTTTACGCTGCTAAAT *F CGCCTGTCCACCATCGACGAGAAACTGCAGGTGAAGACGGAGCATGAGCT *F CACAACCGACAACACTGCGCTCTACCTAGAGCCAAACTTCGAAGAGAAGC *F TGGTTGCATATTGCCACAAGCTGATCAAGCACGCCTGGAGCATGCCCAGC *F ACAGCCGCCTCGTGGACGGTGGGATTACGTGGCATGACTCTGCTCCACTT *F GGCCGCTGCCTTGGGCTATGCCAAGCTGGTGGGCGCCATGCTGAATTGGC *F GATCGGAAAATCCACATATCATTCTTGAAACCGAACTGGACGCCCTCAGC *F CAGGATGTCTACGGTTTCACTCCGCTCGCCTGGGCCTGTGTACGTGGCCA *F TGTGGAGTGCTCGCTGCTACTCTACAAGTGGAACCACAATGCGCTGAAGA *F TCAAAACGCAGGCACAGCAAACTCCCCTGGATTTGGCCAGCATGCGGGGT *F CACAAAGTCCTGCTGGCGCAGATGTATCGCCTGGAGAAGGAGCGATGCCC *F GGAGGCGGAGGAACAGCACCAGAGTTTCAGCGCTTTTGACTTGGAACTCC *F AGCGAAAGCATGATGGAGTTTTCCTAAGACCTGTTGCGGTGCATAGCAAT *F CAGAGTCCACCCAATGGCAGCAGTCGTTACTCCAAACGTTCGTCCATAGA *F TAGTGGCATTAACATGGATATACGCAGCAAGTCTGGAAAACCGCTGGCCA *F GGCTCCATAGCAACTTTGAAAGCCATGACAGCTATGCTCTGGGCGTCGAT *F TCGCCGCTGGACTCGCTGACTGGAACCAACTGTCTGCTGTCACCGCTGCG *F CAAAATGGACTTTGCCCTTTGCGAGGTATCTACGGGCGAATCGAGTCCCG *F TGCACGACAAAGATTGCGACGACAATTCAACAAGCGCGACCGACGTGACC *F ATTGGCAATGATTTGGTCCTGCCCGATGCCGTTGTAGGGGACTCCGATGC *F CAAAGTTCTGACATTAGCTGAACACATAATAGCAGCCATGCCAGAACGAA *F TCAAGAACGAAGCCGATGAAATGATGGTGCTGGGCAGTCCCTTGACAGAA *F CCCCTCACTTCAGAATCTTCAGCGCTGACGGACAGCTTTATGGACCCACT *F GCTGGATTCGCTGCCCAACACACATTTCGACAGCGACTTCAGCTTCGACT *F TCCATGACCATAGCTATCGCTATCACGACGTCAGCACGCCGTGCTCCAGT *F TTGAGTCCCGCCAGCTCGGGACCGCTGCAGTCCCCGGCCAGTTACTCTAT *F TTTGGGAACCGATCCCTCAGTCAGTTCACCCAGTCCCCCGCCATCCACCA *F AACAGTTGACGGAGTTCCTGCACGCCTCCAGCATCTCGTCGTATCCCTTT *F GAGGCGGATTTCTCCAAGCTAACCCTAACAGACACGGAACAGCGAGAGCT *F TTACGAGGCAGCCAAGTGCATCCAGAAGGCGTATCGCTCGTACAAGGGTC *F GACAGAAGCTCGAAGAACAGAACAAGGAACGGAGCGCTGCCACTGTCATC *F CAAAACTACTATAGGCGGTACAAGCAGTACGCCTACTACAGGCAAATGAC *F AAATGCTGCCCTGGTGATTCAACATGGCTATCGCTCCTACAGACGAAACA *F AGCGATTCAAGAAGAGCGGCTTGTGCTTGAGCAGCTCCAGCGATCATGGA *F AGTGTGAGCAGCAATTCTCAGTGCCTGTCCAGCTTCTACGATCACTACAA *F ACAGGATCAGCAGCAGCTTCACGAGTTGGGCTCCCAACCGAGCACGCCTA *F AGGAAACCAGCCCTTCGGGTCCCTTAAAGCGAACCTACTCCCAGTCCACG *F CAAAATCAAGCTGCCCGGAAAATTCAACAGTTTATGAGACAATCACGCAT *F CAAACTACAGAAAGAGCGAGCCGAAAAAGAGAAGCTGGTGCACCAACGCA *F GGGCGGAATACCTTCAAAACTTGCAGTTTCAAGGGCAGCAGGAAATGTTG *F GTCTACCACGAAAATAACATTTCTGCGCCAAGCAGTGGCAATACCAATGC *F GAGCAACAACAACAATCTACACCAAATACAATCCAATCAGTGA *F Comments: CG8809 is one member of the CAMTA (Calmodulin \-binding *F transcription activator) family of proteins found in human brain, fly, *F nematode and other plant species. The proteins are transcription factors *F targeted to the nucleus containing a calmodulin-binding domain and ankyrins *F (ANK) motifs. Function remains unknown. *F CAMTA proteins are described in the following article : *F 'A novel family of calmodulin-binding *F transcription activators in multicellular organisms' that has been accepted *F and will be published in Journal of Biological Chemistry by Nicolas Bouché, *F Ariel Scharlat, Wayne Snedden, David Bouchez and Hillel Fromm. *F We refer to CG8809 as DmCAMTA. # *U FBrf0149572 *a Edwards *b K. *t 2002.3.15 *T personal communication to FlyBase *u FlyBase error report for CG9488 on Thu Mar 14 17:37:11 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 14 Mar 2002 17:37:11 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: kaedwar@ilstu.edu *F Subject: FlyBase error report for CG9488 on Thu Mar 14 17:37:11 2002 *F Error report from Kevin Edwards (kaedwar@ilstu.edu) *F Gene or accession: CG9488 *F Release: 2 *F Gene annotation error *F Gene CG9488 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Ddr virtual cDNA *F GAACCTGCAAACAGGCCTTGGGCATGGAGTCTGGCGCCATCGCCGACTTTCAAATAACGGCCAGTTCGGCCCATGATAT *F GGGAAATGTGGGACCACAGCATGCCAGACTTAAGATAGACAACAACGGAGGCGCTTGGTGTCCCAAGCACATGGTATCT *F CGTGGTCTGACGGAGTACCTGCAAGTCGACCTGCTAGGTGTCCACCTGGTCAGTGCCATACGAACCCAGGGTCGCTTTG *F GCAAGGGGCAGGGCCAGGAGTATACCGAAGCCTATGTGATTGAGTACTGGAGGCCGGGCCTCAAGAAATGGATTCGATG *F GAGGAGCCTCCAAGGCAAAGAGGTGCTGCCCGGCAACATAAACACCTACAGCGAGGTGGAGAACGTGCTCCAGCCCAGC *F GTCTTTGCGTCCAAGGTGCGACTCTATCCCTACAGCCAGTACGATCGCACCGTCTGCCTGCGCGCCGAGATCGTCGGCT *F GTGCCTGGGAAGAGGGAATTGTATCGTACAGTATTCCCAAGGGCATGCAGAGGGGCATGGACATCGATCTGTCGGACAA *F AACCTACGATGGATACGAGGAGGGTGATCATTATGTCAACGGTTTGGGCCAACTTGTTGATGGTCAGCGGGGCAAGGAC *F AACTTTCGAGCCGATATTAATGGCCTTGGCAAAGGTTACGAATGGATTGGCTGGCGAAACGACACTTTGCTCGGGCGTC *F CAGTTGAAATCATATTCGAGTTTGAGACAGTTCGGAATTTAAGTGCTGTTATCATACACACCAACAACATGTTTTCCAA *F AGATGTTCAGGTTTTCGTCCATGCCAAAGTGTTTTTCAGCATTGGCGGCCGCCACTTCTCTGGAGAGCCGGTGCAGTTT *F TCCTATATGCCCGATCTGGTTTTGGATCATGCACGCGATGTGACAATCAAGTTGCATCATCGTCTGGGGCGCTACGTGC *F AGTTGCATCTATACTTTGCCGCCCGCTGGATGATGATAAGC! *F GAGATAACGTTCATATCAGTTATATCTCCGAAGCCTATTGACCACCAGGAACCGGAGACGAGCTTCGTCGGCGTCATAA *F TCACAGTGCTGGGCACAATTATAATGCTGCTGGTGGCATTTATACTACTGATAGTGGCTCGCAACAAGCACGTTAGGAG *F CAGGGAGAACGGATTGGATGCCTTCCAGCACAACTTCAGCCCGGACACACTTAACGGCAACGGTGTCCTGAAGGTGGTC *F ACAACAATGGATGACAATGAGTCGTCCATTGACAAGAACAGTCTGTACCATGAGCCCTTCAACGCGAACATGTACACCA *F GTGCGGCGAGTGCTTGCCCCATGAATGACCTGCAGCGTCAGCATGTATCACCCGACTATACAGATGTGCCGGACATTGT *F GTGCCAGGACTATGCGGTGCCTCACATGCAGCAGCTGCTGCCCAATGCCCCGCCATGCGGTTCGGGATGCGGAACAGGC *F ACGGGCACAGGTCCTGGAAGGGGGTCGAGTCCTGAATTCGTTGTTGCCACAGGCAAAGTGACCGCCAGCGCACGCAACT *F CTCTTAATGCGGCCACACTGCCGCTACCTTCGCCGCCCGTGCCACCGCCGCTGGAGAAATATTACGCAGCAACGCCAGT *F TAGCAGCAAACCCATGCCAGTGGCGCCGCCGGGATCCCAGAGTAGTGGATCCCTTAGTTCTTCGACTACGGCTGCGACC *F ACGCCCACCAGTGGGGTGGTGGGCGTAGGCAAACCGCATCATTACAATTTGGATATGAGCGCAAATTTCGCCGACATTA *F ACGAGGAGCGGGCCAATTGCCAAGTGCAGGAGTTTCCACGTCAGTCGCTGGTCATCGTCGAGAAACTGGGCTCCGGCGT *F ATTTGGTGAGCTGCATTTGTGCGAAACAAATGTGCTGAATGATCTAACCGAATTTGATTCCCATTGCAGCGCTACCCTC *F GTGGCAGTGGCCACCCTGCGTCCCGGGGCCAATGACCATCT! *F GCGTAAGGAATTCCGCAGCAAGGCAAAGCAGCTGGCGCAGCTGAGCGACCCCAATGTGGCCCG! *F CTTGGTGGGTGCCTGCCTGCGGGACGAGCCCATCTGCATTGTTCAGGACTACTCGCACTGCCTGGGCGACTTGAACCAG *F TTCCTGCAGGAGCACGTGGCCGAGACCAGCGGACTGATGGCCAAGAAGAGTCTTAGCTTCGGGTGCTTAGTCTACATTG *F CCACCCAAATTGCATCGGGCATGAAGCATTTGGAGCAAATGAATTTCGTTCATCGGGATCTGGCGACAAGGAGCTGCAT *F CATTGGCCCCGAACTGTGCGTCAAAGTCTGCTCCATCGGCACTGTGATAAACCGCTCGGCCTACGCGTCGGACTACTGC *F CAATTGGAGGGCTTCACCGGCAGACAGAGCCAGCCCATGCCCATTCGCTGGATGGCTTGGGAGAGCGTCCTATTGGGCA *F AGTTCACAACGAAATCGGATGTGTGGTCCTTTGCGGTTGCCCTGTGGGAAATATTGACTTTCGCCAGGGAGCAGCCCTA *F CGAGCATTTGTCGGACAAAAGTGTAATTGAAAACATCGGCCTCATATATCGGGACTACAAAATGCATGAACTTTTGCCA *F ATGCCTCCGAATTGTCCGCGAGAGATTTACGATCTGATGTGCGAGTGCTGGCAGCGCGACGAGTCGAGTCGACCCAGTT *F TCCGCGAGATACATTTGTATCTGCAGCGCAAGAATTTAGGTTTTAAGCCACAGACCCATACACACATGTATTGA *F Protein sequence: *F >Ddr virtual protein *F TCKQALGMESGAIADFQITASSAHDMGNVGPQHARLKIDNNGGAWCPKHMVSRGLTEYLQVDLLGVHLVSAIRTQGRFG *F KGQGQEYTEAYVIEYWRPGLKKWIRWRSLQGKEVLPGNINTYSEVENVLQPSVFASKVRLYPYSQYDRTVCLRAEIVGC *F AWEEGIVSYSIPKGMQRGMDIDLSDKTYDGYEEGDHYVNGLGQLVDGQRGKDNFRADINGLGKGYEWIGWRNDTLLGRP *F VEIIFEFETVRNLSAVIIHTNNMFSKDVQVFVHAKVFFSIGGRHFSGEPVQFSYMPDLVLDHARDVTIKLHHRLGRYVQ *F LHLYFAARWMMISEITFISVISPKPIDHQEPETSFVGVIITVLGTIIMLLVAFILLIVARNKHVRSRENGLDAFQHNFS *F PDTLNGNGVLKVVTTMDDNESSIDKNSLYHEPFNANMYTSAASACPMNDLQRQHVSPDYTDVPDIVCQDYAVPHMQQLL *F PNAPPCGSGCGTGTGTGPGRGSSPEFVVATGKVTASARNSLNAATLPLPSPPVPPPLEKYYAATPVSSKPMPVAPPGSQ *F SSGSLSSSTTAATTPTSGVVGVGKPHHYNLDMSANFADINEERANCQVQEFPRQSLVIVEKLGSGVFGELHLCETNVLN *F DLTEFDSHCSATLVAVATLRPGANDHLRKEFRSKAKQLAQLSDPNVARLVGACLRDEPICIVQDYSHCLGDLNQFLQEH *F VAETSGLMAKKSLSFGCLVYIATQIASGMKHLEQMNFVHRDLATRSCIIGPELCVKVCSIGTVINRSAYASDYCQLEGF *F TGRQSQPMPIRWMAWESVLLGKFTTKSDVWSFAVALWEILTFAREQPYEHLSDKSVIENIGLIYRDYKMHELLPMPPNC *F PREIYDLMCECWQRDESSRPSFREIHLYLQRKNLGFKPQTHTHMY* *F Comments: The 5 CG's from CG9488 to CG9490 are rerally all one gene, a *F receptor tyrosine kinase gene we call Ddr (for the DDR class of RTKs). We have *F no cDNA for it, and I haven't found any ESTs, but the gene structure I put *F together is mostly unambiguous (based on similarity to other RTKs, and to a *F partial tandem duplication of Ddr that lies ~100kb upstream.) There are a few *F questionable splices, but overall it lines up well to the human ortholog. The *F first exon, which presumably encodes the signal sequence, can't be identified, *F so my prediction begins with what is probably the second coding exon. The *F introns are huge, but I did not detect any alternative exons similar to the *F ones used. # *U FBrf0149573 *a Furukawa *b Y. *t 2002.3.21 *T personal communication to FlyBase *u FlyBase error report for CG5830 on Wed Mar 20 17:11:30 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Wed, 20 Mar 2002 17:11:30 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: yoshi@biol1.bio.nagoya-u.ac.jp *F Subject: FlyBase error report for CG5830 on Wed Mar 20 17:11:30 2002 *F Error report from furukawa (yoshi@biol1.bio.nagoya-u.ac.jp) *F Gene or accession: CG5830 *F Release: 2 *F Gene annotation error *F Gene CG5830 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F ACACGTTTTGTTTTTTGCGCTATTCCTGTTACTTTTTTTCGACGTACACACGCGTTTTGTTATTTTGCGCGATTAATTA *F ATGGCAAAAAATAAAAAAAATAAATAAATAACGAGACGAAATAACGAAAACACCAGCGGCCGAGCTAGTGTGCGTGCGA *F GAGTGTGTGTGAGTGCTGTGTCTGTGTGGGCGTTTATAAATAAAACGCTGCGAAGCTGTGTGCGAAAAGCAGACGCGGA *F TAAAGAAGTAAAAGAATGAGCGCAACCAAAAACAAGAGCGAAGGAAAGAAAGAAAGTGTTTTTTATTGAAATTCGTTGA *F AAAGTTTTAAATTTACTAAAACAGAGAGGCGAAAGTGCAACAGAGCGAAAGAGAGAGCGATAAATAGCGAGCTATCCTA *F TACGAAACCGATCTGAATCAGTATCTGAATCCGAATCCGATTTATCAACCTGTTAAGCGAAGTGGAGCCACAAACCGAG *F TGCTTAAATGGATGCAACATCAATAATAACGCAAGTTTCCCGCGACGACGAGCAACTGAACGTCTATCCCAGCTATCCC *F AATGACAAAGACGATGTGGATCGTTTAAAGCCACAGAAGCGAGGATTATTCCATTCTCTGCTTTGCTGCTGGCGACGAA *F ACCGAACGAAAACCAACCAGAATGGCACACAAATCGATGGATCGACAACACCGCCACCATTACCGGACCAACAAAGGTA *F CCTACTACCTCAGGTTAGGCTTACCGATATGCACAGAAAGTGCATGGTGATCGATTTGGATGAGACCCTAGTGCACAGT *F AGTTTTAAGCCCATACCGAATGCTGATTTCATAGTGCCCGTGGAGATCGATGGAACCGTACATCAGGTGTACGTCCTGA *F AGCGACCGCACGTGGACGAGTTTCTGCAGAAGATGGGCGAACTATACGAGTGCGTTCTGTTCACAGCATCACTAGCCAA *F ATACGCAGATCCCGTCGCCGATCTGTTAGACAAGTGGAATG! *F TGTTTCGTGCAAGACTGTTTAGGGAATCATGCGTTTATTACAGGGGTAATTACATTAAGGATCTGAATCGTCTGGGGCG *F GGACCTTCAGAAGATTGTCATTGTCGACAATTCACCGGCCAGTTATATCTTTCATCCAGACAATGCAGTTCCTGTTAAA *F TCCTGGTTTGACGATGTCACCGACTGCGAACTGCGCGAACTGATCCCGCTCTTTGAGAAGCTTAGCAAAGTTGATTCCG *F TCTACTCGGTGCTCTGCAATTCGAACCAGCCGCTGAACAATCAGACAAACCAGCAGCAGCATCCCCAGGAACTGCAGCA *F GGCGCCGAACCAGCTGCATCAGCAGCTCCAACAGCAGCAACAACAACAGACCATCTCTGCCACGACAGTTATTACCCAG *F GCAACCACATTGTCTGCTCCGACCATGTTGAACCAGCAGCAGACAAGTCCGCCCAGCCCACAAAGCGAGTTGCTGCAAA *F AGACGTAACATCAGTGGGAACTAATGTTTATAAATATATGATAAATTGTTTACATATTACAAGACCAAGCAAGCCGATA *F AGCAAGCAAACAAAGCATCCCTATCCCAAGCAGTTCACTCAGTACCCCAGGACGAATGGTAATGGGTCGACGAGATGAA *F ATCAACTACCAATTGCTATCAACTATAATCCCCAGATCGACACCTAGGCCTAAGCATAAGTTTTATATGATATACATAA *F TTTTTACAGAACCCAATTTACACGGCACATTTGTTATTTATTCTTGTTTTGCTCTAAACCAATTATAATAATTATTATT *F TTTTTGTGTTTTTTCTAAACAACTGCTTATTGCTTACTGCGTTTTGCGGTACCCCAACTAGGGGGTATATTAAATCCAA *F GCATAAGCCTCTATTTTTACCCAATAATCATAACTCTGTAGACGTATTTAATGGTAAACCAATTGAGCAAGCAAAAGAT *F TTAAATGCATAAAACAAAAATGATAACTATACATATGAACA! *F GAGAGATAAAATCAAATTCAAAATAGCTATATTTATATATATTTTTTATTAAAAAAACAAGAA! *F AACAAAAA *F Protein sequence: *F MDATSIITQVSRDDEQLNVYPSYPNDKDDVDRLKPQKRGLFHSLLCCWRRNRTKTNQNGTQIDGSTTPPPLPDQQRYLL *F PQVRLTDMHRKCMVIDLDETLVHSSFKPIPNADFIVPVEIDGTVHQVYVLKRPHVDEFLQKMGELYECVLFTASLAKYA *F DPVADLLDKWNVFRARLFRESCVYYRGNYIKDLNRLGRDLQKIVIVDNSPASYIFHPDNAVPVKSWFDDVTDCELRELI *F PLFEKLSKVDSVYSVLCNSNQPLNNQTNQQQHPQELQQAPNQLHQQLQQQQQQQTISATTVITQATTLSAPTMLNQQQT *F SPPSPQSELLQKT *F Comments: I finished sequencing EST clones (RE52350 amd SD03472). *F Therefore I found a mistake in the intron-exon structure. # *U FBrf0149574 *a Buenemann *b H. *t 2002.3.21 *T personal communication to FlyBase *u *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 21 Mar 2002 08:47:34 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: bueneman@uni-duesseldorf.de *F Subject: FlyBase error report for CG3723 on Thu Mar 21 08:47:34 2002 *F Error report from Bünemann Hans (bueneman@uni-duesseldorf.de) *F Gene or accession: CG3723 *F Release: 2 *F Assembly error *F Comments: By a very careful comparison with the sequence of the orthologous *F gene DhDhc3 of D. hydei we found several corrections for the gene-region fasta *F display. We have corrected this file and I would like to send it as the word *F Dhc93ABcor.doc file. In this way the exon/intron specific colors of the *F 'Gene-region fasta display' are conserved and the changes are shown by *F underling. I suppose that this way of correction is much more convenient and *F less error-prone for complex changes in large genes than your recommended *F stepwise procedure. *F Please let me know to which address I can send an attachment of the *F Dhc93ABcor.doc file. Yours sincerely, Hans Buenemann. *F FlyBase curator comment: Dhc93ABcor.doc word file is archived # *U FBrf0149575 *a Bilder *b D. *t 2002.3.26 *T personal communication to FlyBase *u FlyBase error report for CG18338 on Tue Mar 26 13:16:04 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 26 Mar 2002 13:16:04 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: Bilder@socrates.berkeley.edu *F Subject: FlyBase error report for CG18338 on Tue Mar 26 13:16:04 2002 *F Error report from David Bilder (Bilder@socrates.berkeley.edu) *F Gene or accession: CG18338 *F Release: 2 *F Gene annotation error *F Genes CG18338 and CG13219 should be merged. *F Gene cDNA sequence: *F >skiff cDNA *F CCGTCGTGTGCAGTCGCTCTCGCTCCCGTTATTCGCCGTTCTGGGATTCCCGCTGGCGGACTCTGGTGGCCATCACCTT *F GATAATCCTTATTGTGAAATATGTGAAGTGCTGCAAAGAGTCTAAGGATATCCTGATATATTAACGGTTAACTAAACTA *F GTGCGCAATATTAGAGGCCTAAAAATAGCGAAAATAGCAGACATGCCGTGTTTTTGTGCCTCTGTGTGTGCGTGAGTGT *F CTGCGAGTGAAGTGGAAATTTAAAAATAAAACAACGCTGGGACTCAACCGAAATCCGTCTGTATCGTATTGGCCAACTG *F CCCAGAAAAACTCCAGGAACAGCGCCAAAGAATAACTTGTAAAGGATAAGCAAGGACAGTGACTCGTCGACTGTTGTGT *F GACATGCTAGAAGCAGTTATGGCATCGGACATCAACTGGGATCCTGCTCTTTCCAAGCTGATCAGCACGCTCAAGGAGT *F CGGAGAATCTATCCAATGACCAGGAAATCGACTTCCTGAAGGCCTTGCTTGAATCCAAGGAACTAAACGCGCTGGTCAA *F TGTCCACACGAAGGTGGCCAAAGTGGGTCGAGATGATCGTCTGGCCCCGGTGCTCTCCACCTCTGCCCAGGTGTTGTAC *F GAAGTCCTGGAGCAGCTGTCCCAGCATTGTCATCTGAACGATGACTGCAAGGAGGCCTTTCACCTGCTGCAGGACTCTC *F ACCTGCAGCACCTTCTGTTCGCCCACGATGCGATTGCCCAGAAGGACTTCTACCCGCACCTGCCGGAGGCGCCTGTCGA *F AATGGACGAGGACGAGGAGACCATCAAGATTGTGCAGCTCGTCAAGAGCAACGAGCCCCTGACAGGAGCACAGAGTACG *F GAGCCAATTGTTGGCGCCACCATTAAAACGGACGAGGAGTCGGGCAAGATAATCATCGCCAGAATTATGCACGGCGGCG *F CTGCAGATCGCTCCGGATTGATTCACGTCGGCGACGAGGTC! *F ATCGAGGTCAACAACATCAACGTGGAGGGCAAGACGCCGGGCGATGTGCTCACCATACTGCAAAACTCGGAGGGCACCA *F TTACCTTTAAGCTGGTTCCCGCGGACAACAAGGGCGCCCAGCGCGAGTCCAAAGTGCGGGTGAGGGCCCACTTTGACTA *F CAATCCGGATGTGGATCCCTACATTCCGTGCAAGGAGGCGGGTCTGGCCTTCCAGCGCGGCGATGTGTTGCACATTGTG *F GCCCAAGACGATGCCTACTGGTGGCAGGCGCGCAAGGAGCACGAGCGCTCGGCAAGAGCCGGTTTGATTCCCAGCCGGG *F CGCTGCAGGAGCGCCGTATCCTCCACGACCGTACACAGAAAAACGGCACCGATCTGGACTCGAAGCAGAACGACGACTT *F TGACCGCGAGCAGATCGCCACGTACGAGGAGGTGGCCAAGTTGTATCCACGGCCCGGAGTCTTCAGGCCCATAGTGCTC *F ATCGGAGCACCCGGCGTGGGGCGGAATGAATTGCGCCGCCGGCTGATAGCCCGGGATCCCGAGAAGTTCCGCAGTCCGG *F TTCCGTACACCACCCGACCAATGCGCACAGGTGAAGTGGCCGGACGAGAGTACATCTTTGTGGCCCGCGAGAAAATGGA *F CGCGGACATCGAGGCGGGAAAGTTTGTGGAGCACGGCGAGTACAAGGGCCATTTGTACGGAACATCGGCGGAGAGTGTC *F AAGTCGATTGTGAATGCCGGCTGCGTATGTGTGCTGAGTCCGCACTACCAGGCGATCAAGACTCTGCGCACAGCTCAGC *F TGAAGCCCTTCCTCATCCATGTGAAGCCACCAGAGTTGGACATCCTGAAGGCTACGCGAACGGAGGCGCGTGCCAAATC *F CACATTCGACGAGGCGAATGCCAGAAGTTTTACGGACGAAGAGTTCGAGGACATGATCAAGTCGGCCGAACGCATAGAT *F TTCCTATACGGACACTTCTTCGACGTAGAGCTGGTCAACGG! *F CGAACTGGTCAATGCTTTCGAGCAGCTGGTCCAGAATGTCCAGCGACTGGAGAACGAACCGGT! *F ATGGGCACCATCCATGTGGGTGCAGTAGATCGGTTGCCAAATGTGGACAAGCATTTCATGCAGTTTGTTGATGAACACC *F AAAGTATTTTTGCCATACGAATTTAGCAATCTGCTAGAGAAACGGAGAAGAGACACTATTTACCCGTAGGCGGTCCTGC *F TGGGCACTCCCGTCCGGCCTTAGTCCTTTTCACGACATGTACCTTTTTGCGTAACAGTGAAAACAATGTAATGAATACA *F ATAATTATTGACAGAAAGTGAAGGAAATTTGAACTCAACCAGCATCTAACTTACAATTAAATTATATAAACTTGTTGTA *F CAAAATTTATTTGTGTTCAAAGAATGACTTAATTATAAATGTATGTATTACACGTTGTTGAAATCAAAGTACAGAGTTA *F ATCCCCCCGTAAAACCTAAAGCTAATCGTATTGTATCTCAGTTGTATATACACTCACTAACTGAAGCATATTGGATACA *F ATGTGAAGTTCTGTGTGACGACATGTCTCATTTCGAAAACTGATTTCACCAAAAACGTAGTGACAAATTGTTGAAAACA *F AGTGAGAAACTAATAAAAAAAAAAAA *F Protein sequence: *F >Skiff protein *F MLEAVMASDINWDPALSKLISTLKESENLSNDQEIDFLKALLES *F KELNALVNVHTKVAKVGRDDRLAPVLSTSAQVLYEVLEQLSQHCHLNDDCKEAFHLLQ *F DSHLQHLLFAHDAIAQKDFYPHLPEAPVEMDEDEETIKIVQLVKSNEPLTGAQSTEPI *F VGATIKTDEESGKIIIARIMHGGAADRSGLIHVGDEVIEVNNINVEGKTPGDVLTILQ *F NSEGTITFKLVPADNKGAQRESKVRVRAHFDYNPDVDPYIPCKEAGLAFQRGDVLHIV *F AQDDAYWWQARKEHERSARAGLIPSRALQERRILHDRTQKNGTDLDSKQNDDFDREQI *F ATYEEVAKLYPRPGVFRPIVLIGAPGVGRNELRRRLIARDPEKFRSPVPYTTRPMRTG *F EVAGREYIFVAREKMDADIEAGKFVEHGEYKGHLYGTSAESVKSIVNAGCVCVLSPHY *F QAIKTLRTAQLKPFLIHVKPPELDILKATRTEARAKSTFDEANARSFTDEEFEDMIKS *F AERIDFLYGHFFDVELVNGELVNAFEQLVQNVQRLENEPVWAPSMWVQ *F Comments: CG18338 and CG13219 are two portions of a single gene, which encodes *F a p55-like MAGUK subfamily member that we have named Skiff (Skf) (Genbank *F AF495381). *F We have sequenced three cDNAs (including GH12103) and found that they contain *F sequences of both predicted genes. Examination of genomic splice *F acceptor/donor sites is consistent with the gene merge. The predicted *F full-length Skf protein joins L27 domains from CG18338 with the PDZ, GUK, and *F SH3 domains from CG13219, and the resultant protein is more homologous to *F zebrafish Humpback then either individual predicted protein. Northerns and *F embryonic in situs using probes for either predicted gene show similar *F patterns. Finally, mutations that affect CG18338 transcription also affect *F CG13219. # *U FBrf0149576 *a Mueller *b J. *t 2002.3.28 *T personal communication to FlyBase *u FlyBase error report for CG10852 on Thu Mar 28 15:55:55 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 28 Mar 2002 15:55:55 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: jlm62@cornell.edu *F Subject: FlyBase error report for CG10852 on Thu Mar 28 15:55:55 2002 *F Error report from Jacob Mueller (jlm62@cornell.edu) *F Gene or accession: CG10852 *F Release: 2 *F Gene annotation error *F Gene CG10852 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Acp63F *F ATGAAAGCTATCATCGTTTTTATTCTGTTCATTTCAAGTGTGCATGCTAT *F GAGCAAATGCAACCAAGCAATTTATCTAAATCTTGATCCTCACTGCGGAA *F TACTTCCCGATTGTAACTTAGATGGTCCAAATCCAAGTTACCTCAAAAGG *F GTGTCGTGTGAACGCAAAGAAAACGGAAAACCAGGATTCATCGAACTAAT *F TCCCGGAAAATGTCTCCATGGTAAACCGCGTTGCTCGTTAAAATAG *F Protein sequence: *F >Acp63F *F MKAIIVFILFISSVHAMSKCNQAIYLNLDPHCGILPDCNLDGPNPSYLKRVSCERKENGKPGFIELIPGKCLHGKPRCS *F LK *F Comments: The signal sequence is missing, you have it in the cDNA as part of *F the UTR, but don' t have it in the AA seq. or CDS # *U FBrf0149577 *a Sampson *b H. *t 2002.5.10 *T personal communication to FlyBase *u *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Fri, 10 May 2002 11:30:33 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: heidi.sampson@utoronto.ca *F Subject: FlyBase error report for CG4059 on Fri May 10 11:30:33 2002 *F Error report from Heidi Sampson (heidi.sampson@utoronto.ca) *F Gene or accession: CG4059 *F Release: 2 *F Gene annotation error *F Gene CG4059 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F > CG4059 Ftz-F1 *F ATGGATACCTTCAATGTACCTATGCTGGCGGAGAGCAGCAACACCAACTATGCAACAGAAGCTACCAGCAATCATCACC *F ACCTGCAACATCAGCATCAGCAGCAACATTCACATCAGCAGCAGCAGCAGCAGCAACTCCTAATGCCCCATCATCACAA *F GGATCAAATGCTGGCGGCGGGCAGTTCGCCCATGCTGCCATTCTATTCACACTTGCAGTTGCAACAGAAGGATGCAACT *F GCAACAATAGGACCTGCAGCAGCAGCAGCAGCAGTTGAGGCGGCAACAACATCGGCAAATGCCGATAACTTCAGTTCAT *F TGCAAACGATCGATGCCAGTCAACTGGATGGAGGCATCTCCTTGAGCGGCTTGTGCGATCGTTTCTTTGTGGCCAGTCC *F AAATCCCCACAGCAATAGCAACATGACACTAATGGGCACTGCCACAGCTGCAACAACTACCACTACCAACAACAACAAT *F AATAACAACACTAACAATAACAATAACAACAACGTGGAGGCTAAGACAGTGAGGCCATCGAACGGCAATTCGGTGATCA *F TCGAATCTGTGACGATGCCATCGTTTGCCAATATTCTATTTCCCACCCATCGCAGCGCCAACGAGTGCATTGATCCCGC *F CCTGTTGCAAAAGAATCCCCAAAATCCAAATGGCAACAACAGTAGCATAATTGTGCCGCCCGTCGAATATCATCAATTG *F AAACCACTTGAAGTCAATTCGTCGACTTCCGTTTCCACCAGCAACTTCCTGTCCTCCACAACGGCCCAATTGTTGGACT *F TTGAGGTGCAGGTGGGCAAGGATGATGGCCACATTAGCACCACTACGACCACAGGGCCGGGCTCTGGATCGGCATCGGG *F TTCAGGATCGGGATCGGGATCGGGATCAGGATCGATTGCACGCACAATAGGCACTGCAACGCCCACAACGACAACATCC *F ATGAGCAACACTGCCAATCCGACGAGGAGCTCATTGCACAGTA! *F TTGAGGAGCTGGCCGCCAGTTCCTGTGCCCCGAGAGCAGCGTCTCCCAACAGCAACCACACTAGCAGTGCCAGCACCAC *F TCCGCAGCAGCAGCAGCAGCAGCAGCATCACATGCAAAGCGGCAACCACAGTGGCTCCAATCTCAGCAGCGACGATGAG *F TCCATGTCCGAGGATGAGTTCGGTCTGGAAATCGACGATAACGGAGGCTACCAGGACACCACCTCCTCACACTCGCAAC *F AGAGCGGAGGAGGCGGTGGCGGCGGCGGTGGCAACCTGCTAAACGGCAGCTCCGGCGGCAGCTCCGCCGGCGGTGGCTA *F CATGCTGCTCCCCCAGGCGGCCAGCTCCAGTGGCAATAATGGCAATCCGAATGCCGGCCACATGTCCTCCGGTTCCGTG *F GGCAATGGCAGCGGAGGCGCTGGCAATGGCGGAGCGGGCGGCAACTCCGGTCCCGGCAATCCCATGGGCGGTACGAGCG *F CCACGCCGGGACACGGCGGCGAGGTGATCGACTTCAAGCACCTGTTCGAGGAGCTTTGCCCCGTGTGTGGCGACAAGGT *F GAGCGGCTACCACTACGGCCTGCTCACCTGCGAGTCCTGCAAGGGATTCTTCAAGCGCACCGTGCAGAACAAGAAGGTC *F TACACCTGCGTGGCGGAGCGGTCGTGCCACATCGACAAGACGCAGCGCAAGCGGTGTCCCTACTGCCGATTCCAGAAGT *F GCCTCGAGGTGGGCATGAAGCTAGAGGCTGTTCGAGCGGATAGAATGCGTGGTGGACGCAACAAATTCGGACCCATGTA *F CAAACGGGATCGCGCGCGGAAGTTGCAAGTGATGCGGCAGCGGCAGTTGGCGCTGCAAGCGCTGCGCAACTCGATGGGT *F CCGGACATCAAGCCAACGCCGATCTCGCCGGGCTACCAGCAAGCATATCCAAATATGAACATTAAGCAGGAAATTCAAA *F TACCTCAGGTATCCTCACTCACCCAATCTCCGGACTCGTCGCC! *F CAGCCCCATAGCAATTGCGTTGGGACAGGTGAACGCGAGCACGGGCGGTGTTATAGCCA! *F CGCCCATGAACGCCGGCACTGGCGGCAGTGGGGGCGGTGGTCTGAACGGACCAAGTTCCGTGGGCAACGGCAATAGCAG *F CAACGGCAGCAGCAACGGCAACAACAACAGCAGCACGGGCAACGGAACGTCCGGAGGAGGAGGTGGCAATAATGCGGGC *F GGCGGAGGAGGAGGAACCAATTCCAACGATGGCCTGCATCGCAACGGCGGCAATGGCAACAGCAGTTGCCACGAGGCTG *F GAATAGGATCTCTGCAGAACACGGCCGACTCGAAATTGTGCTTCGATTCTGGCACACATCCATCGAGCACAGCCGACGC *F GCTAATCGAGCCATTAAGAGTCTCACCGATGATTCGTGAATTTGTGCAATCTATTGACGATCGGGAATGGCAGACGCAA *F CTGTTTGCCCTGCTGCAGAAGCAAACCTACAACCAGGTGGAAGTGGATCTCTTCGAGCTGATGTGCAAAGTGCTCGACC *F AGAATTTGTTCTCGCAAGTAGACTGGGCACGGAACACCGTCTTCTTCAAGGATCTGAAGGTCGACGACCAAATGAAGCT *F GCTGCAGCATTCCTGGTCGGACATGCTTGTTCTGGATCACCTGCATCATCGAATCCATAACGGCCTGCCCGACGAGACG *F CAACTGAACAATGGTCAGGTGTTCAATCTGATGAGTCTGGGTTTGTTGGGAGTGCCACAGCTGGGCGATTACTTCAACG *F AGCTGCAGAACAAGCTGCAGGACCTGAAATTCGATATGGGCGACTATGTCTGCATGAAATTCCTAATCCTGTTGAATCC *F AAGTGTACGGGGTATTGTCAACCGGAAGACCGTCTCCGAGGGACATGATAATGTGCAAGCCGCTTTGCTGGACTACACC *F CTCACCTGCTATCCGTCAGTGAATGACAAATTCAGAGGGCTAGTTAACATCTTACCGGAAATCCATGCCATGGCCGTTC *F GCGGCGAGGATCACCTGTACACCAAGCACTGTGCCGGCAGTGC! *F GCCCACCCAAACGCTGCTCATGGAGATGCTGCACGCCAAGCGCAAGGGATAGAGGCCGGGAGAACGTGACACGGAATAC *F TTAATCATTTATGAAATGTAAATAACAAGGCGGGAAGGCCCTCGGGGCAACCGGGTCATGGAAGGCGAACGAAGGATAC *F AGCAGAATTC *F Protein sequence: *F > CG4059 Ftz-F1 protein *F MDTFNVPMLAESSNTNYATEATSNHHHLQHQHQQQHSHQQQQQQQLLMPHHHKDQMLAAGSSPMLPFYSHLQLQQKDAT *F ATIGPAAAAAAVEAATTSANADNFSSLQTIDASQLDGGISLSGLCDRFFVASPNPHSNSNMTLMGTATAATTTTTNNNN *F NNNTNNNNNNNVEAKTVRPSNGNSVIIESVTMPSFANILFPTHRSANECIDPALLQKNPQNPNGNNSSIIVPPVEYHQL *F KPLEVNSSTSVSTSNFLSSTTAQLLDFEVQVGKDDGHISTTTTTGPGSGSASGSGSGSGSGSGSIARTIGTATPTTTTS *F MSNTANPTRSSLHSIEELAASSCAPRAASPNSNHTSSASTTPQQQQQQQHHMQSGNHSGSNLSSDDESMSEDEFGLEID *F DNGGYQDTTSSHSQQSGGGGGGGGGNLLNGSSGGSSAGGGYMLLPQAASSSGNNGNPNAGHMSSGSVGNGSGGAGNGGA *F GGNSGPGNPMGGTSATPGHGGEVIDFKHLFEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNKKVYTCVAERSCHID *F KTQRKRCPYCRFQKCLEVGMKLEAVRADRMRGGRNKFGPMYKRDRARKLQVMRQRQLALQALRNSMGPDIKPTPISPGY *F QQAYPNMNIKQEIQIPQVSSLTQSPDSSPSPIAIALGQVNASTGGVIATPMNAGTGGSGGGGLNGPSSVGNGNSSNGSS *F NGNNNSSTGNGTSGGGGGNNAGGGGGGTNSNDGLHRNGGNGNSSCHEAGIGSLQNTADSKLCFDSGTHPSSTADALIEP *F LRVSPMIREFVQSIDDREWQTQLFALLQKQTYNQVEVDLFELMCKVLDQNLFSQVDWARNTVFFKDLKVDDQMKLLQHS *F WSDMLVLDHLHHRIHNGLPDETQLNNGQVFNLMSLGLLGVPQLGDYFNELQNKLQDLKFDMGDYVCMKFLILLNPSVRG *F IVNRKTVSEGHDNVQAALLDYTLTCYPSVNDKFRGLVNILPEI! *F HAMAVRGEDHLYTKHCAGSAPTQTLLMEMLHAKRKG *F Comments: There appears to be an intron of 513bp in length included in the *F cDNA sequence. The sequence that should be removed is as follows: *F AAATGGATCAGCAACAGGCGACCGTACAGTTTATATCGTCGCTGAATATATCGCCGTTCAGCATGCAGCTGGAGCAGCA *F GCAGCAGCCCTCCAGTCCCGCTCTGGCCGCCGGTGGCAACAGCAGCAACAACGCGGCCAGCGGTAGCAACAACAACAGC *F GCCAGCGGCAACAACACCAGCAGCAGCAGCAACAACAACAACAACAATAACAACGACAATGATGCACACGTTCTAACGA *F AATTCGAGCACGAATACAATGCCTACACGTTGCAGTTGGCCGGAGGCGGTGGGAGTGGCAGCGGCAATCAGCAGCACCA *F CAGCAACCACAGCAACCACGGCAACCACCACCAGCAGCAGCAGCAACAACAGCAACAGCAGCAGCAACATCAGCAGCAG *F CAGCAAGAACACTACCAGCAGCAACAGCAACAGAATATCGCCAACAATGCCAATCAATTCAACTCCTCGTCCTACTCGT *F ATATATACAATTTCGATTCACAGTATATATTCCCGACAG # *U FBrf0149578 *a Weake *b V. *t 2002.5.14 *T personal communication to FlyBase *u FlyBase error report for CG15926 on Tue May 14 20:46:23 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 14 May 2002 20:46:23 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: vmw@paradise.net.nz *F Subject: FlyBase error report for CG15926 on Tue May 14 20:46:23 2002 *F Error report from Vikki Weake (vmw@paradise.net.nz) *F Gene or accession: CG15926 *F Release: 2 *F Gene annotation error *F Gene CG15926 has incorrect exon/intron structure or translation start site. *F Comments: Currently the first exon for CG15926 does not match the 5' 239 bases *F of the cDNAs that match the second exon and the rest of the exons *F (e.g.RE29468). Instead the first 239 bases of the RE29468 cDNA match a *F sequence approximately 3500bp upstream of the current first exon (as shown by *F Blast searching). This match for the actual first exon corresponds to position *F 150948 on this particular genomic scaffold X segment, whereas the false first *F exon shown on gadfly is at position 154583. The cDNA sequence itself is *F correct, however the exon/intron boundaries and start site for translation *F shown in gadfly are not (for this first 239 bases). This means there is a very *F large first intron in the CG15926 gene. *F Regards, Vikki Weake, Massey University, New Zealand # *U FBrf0149579 *a Huen *b D. *t 2002.5.16 *T personal communication to FlyBase *u FlyBase error report for CG1200 on Thu May 16 02:23:02 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Thu, 16 May 2002 02:23:02 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: smh1008@cus.cam.ac.uk *F Subject: FlyBase error report for CG1200 on Thu May 16 02:23:02 2002 *F Error report from David Huen (smh1008@cus.cam.ac.uk) *F Gene or accession: CG1200 *F Release: 2 *F Gene annotation error *F Gene CG1200 corresponds to SWISSPROT:AAL50332 *F Comments: The current name assignment of CG1200 to SP512 is based on a *F submission by Serrano et al to Genbank in Feb 2000. Unusually, there appears *F to be a prior submission in Dec 1999 by Taru et al. The publication *F extensively describing the protein product appears to have emerged as:- *F Taru H, Iijima Ki K, Hase M, Kirino Y, Yagi Y, Suzuki T.(2002) *F Interaction of APP family proteins with scaffold proteins of the JNK signaling *F cascade. *F They have been using the name APLIP1 for the protein product and have cloned *F and described a family of proteins which they name JIP of which they identify *F APLIP1 as a member of. # *U FBrf0149580 *a Caggese *b C. *t 2002.5.21 *T personal communication to FlyBase *u FlyBase error report for CG12203 on Tue May 21 04:25:52 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Tue, 21 May 2002 04:25:53 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: caggese@biologia.uniba.it *F Subject: FlyBase error report for CG12203 on Tue May 21 04:25:52 2002 *F Error report from Corrado Caggese (caggese@biologia.uniba.it) *F Gene or accession: CG12203 *F Release: 2 *F cDNA or EST error *F Gene cDNA sequence: *F >RH17496.complete AY070680 *F GGCACTGGCGAACAGCTGTTTTCCCAATCGAAAAGCGCTTGTGTTCCAAATTTTACGTAA *F TTCGTATTTTGAAGCCGATTTCTGCCGTGTAGCCCCAAACGCCTAGCGAAATGTCGGCCC *F TCCGCCAAGTGATGTGCAGAAGCACCGCCTCCCTGCAGCTGTACCAGGCCAACAGGGCAG *F CCGCTGCCCGCTGGGCATCCACCGCCACGGACGGCGGTCCGCTGGACCCCAAGACGGCCC *F TGGCCCGCCCCGAAGAGCTGGAGCAGCGCAACAAGCTGAGTGGCAAGATCACCGTGCCGA *F CTGCCGTCAATCTCAGCCCGATCAGCGGCGTTCCGGAGGAGCACATTCGGGAGCGCCGTG *F TGCGCATCCATATTCCGCCCAAGAACGCAATGCAGAGCGGCACCGATAACGTGAACACCT *F GGCAAATCGAGTTCGACAACCGCGAGCGCTGGGAGAATCCGCTCATGGGCTGGGCCTCCA *F GCGGCGACCCATTGTCCAACATGAACGTGCAGTTCGGATCCCCAGAGGAGGCCATCACGT *F TCTGCGAACGGAACGGATGGCGCTGGTACGTCGACGGCGCCGCGAAGCCCAAGAAGGAGC *F GCGTCAAGAACTACGGCATCAACTTTGCCTGGAACAAGCGCACGCGCGTCTCCACCAAGT *F AGACGCTCCCCGCAGCTAGCCAGGATTATAGACCACAATTATCTGATTTTTTTTTTGCTT *F TGTTCATTATCTGAGTACCCAAATAAACAGTCGGCTGGACT *F Protein sequence: *F >NADH-UBIQUINONE OXIDOREDUCTASE 18 KDA SUBUNIT *F MSALRQVMCRSTASLQLYQANRAAAARWASTATDGGPLDPKTALARPEELEQRNKLSGKI *F TVPTAVNLSPISGVPEEHIRERRVRIHIPPKNAMQSGTDNVNTWQIEFDNRERWENPLMG *F WASSGDPLSNMNVQFGSPEEAITFCERNGWRWYVDGAAKPKKERVKNYGINFAWNKRTRV *F STK *F Comments: Five nucleotides are deleted in the GadFly cDNA sequence for CG12203. # *U FBrf0149582 *a Mansfield *b J.H. *t 2002.5.16 *T personal communication to FlyBase *u *F Date: Thu, 16 May 2002 12:45:07 \-0400 (EDT) *F From: Jennifer Helen Mansfield *F Sender: jhm34@columbia.edu *F To: flybase-help@morgan.harvard.edu *F Subject: update on yps gene *F Hi, *F I'm writing to update your webpage for the yps gene. First, I would like *F to add a primary reference: *F Mansfield, et al., 2002. Ypsilon Schachtel, a Drosophila Y-box protein, *F acts antagonistically to Orb in the oskar mRNA localization and *F translation pathway. Development 129(1): 197-209. *F Second, you have listed two mutant alleles for yps: yps2 and ypsJM2. *F These are actually the same allele, which we published under the name *F ypsJM2 in the paper above. *F Thanks, *F Jennifer Mansfield # *U FBrf0149585 *a Galindo *b M.I. *t 2002.4.9 *T personal communication to FlyBase *u *F Date: Tue, 09 Apr 2002 11:50:47 \+0000 *F Subject: LD11279 and LD20919 *F From: 'Maximo Ibo Galindo' *F To: flybase-updates@morgan.harvard.edu *F Dear Gillian, *F following your suggestion a few weeks ago, I am including our *F sequences *F corresponding to cDNAs LD11279 and LD20919. I am sending them to you *F as attachments in FASTA format. These sequences confirm *F that this locus contains at least two different transcripts sharing *F the *F first exon. This common exon is non-coding and therefore the *F transcripts code for completely unrelated proteins. *F LD20919 confirms the predicted structure for gene CG8165. In contrast, *F LD11279 is not completely identical to the predicted gene CG8176 as it *F appears in Gadfly. When I blasted this sequence against the genomic *F clones it revealed that the gene contains 12 exons instead of the *F predicted 13. *F Ibo Galindo *F \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\Dr. Maximo Ibo Galindo (i.galindo@biols.susx.ac.uk) *F School of Biological Sciences *F University of Sussex *F Falmer *F Brighton, East Sussex BN1 9QG *F UK *F TEL: (44) 1273 877 451 *F ////////////////////////////////////////// *F \------------------------------------------------------------------------------ *F -- *F >LD11279 *F GAAAAATCGAATTTTTCGCTTCAAATCGACTGAAGTTTACACGGGCTAAAGTATTACAGAAGTTTGGCTCAAGCCATTAC *F ACAACATGCAATAAGTAAATTATTTACATAGACAACACTAAGCCTAAGCAAGATACTCTCCAGCAACAAATGCCCATGCA *F TGCTGAGGACGGAGCTGAATCTGATGATGAGGAGTCCAGCCCAGGCAGACATTAGCATCGTAAGGATCATACTACTAATT *F TGGCGCCCATATAGAAGTCAAGTCAATTACAACGCCAGAAAGCCTTTTATGTTAATCGAGAGTGGAAAGGGAAAATAATT *F TTTTTGCTAAACTAGCCTAGAGATCGAAATATTAAGAATACAACTAAAATGACGGTGGATTTCAACGATTATTTTTGGGG *F GGAGAAGAACAATGGCTACGATGTTCTGTACCACAACATGAAGTTCGGCCTGATTGCCAGCAAGGAGTTATCCGAGTTTC *F TGCGCGAGAAGTCTAACATTGAGGAGCAGAACTCAAAGATGATGTCCAAGCTGGCCCACAAGGCTGGTACACTAAATAGC *F ACATTCGCTCCTGTTTGGACCATACTGCGAACATCGGCAGAGAAGCTCTCCACGCTCCACCTGCAGATGGTGCAAAAGCT *F GACTGAGCTGGTCAAGGATGTTGCCAAGTATGCCGACGAGCTGCACAAAAAGCACAAATCCGTCAAGGAGGAGGAATCGC *F AGACTCTTGAGTGTGTCCAAGCCATCCAGACGTCGACGGTGGCAGTGCAAAAGTTGCGCGATCTGTACGCCAGCAAGGTC *F CAGGAGCTGGAGAAGCTGCGCAAGGACAACGGCTCGCACAAGGATGCCGAGAAGCTAGAGAGCAAGCTGAAAAAACTGCA *F GGAGGAGTACAAGGCTTTGTTGGACAAGCATAACCCCATTAAGAACGAATTTGAGAGACGCATGACACAGACCTGCAAGC *F GCTTTCAAGAGATTGAGGAGGTGCACCTTCGTCAAATGAAGGAGTTTCTGTCCACGTATCTAGAAATGCTGCAAAACAAT *F CACGATATGGTGGGTCAAGTACATTCCGACTTTAAGAGGCAGTTTGTCGACATGTCTGTTGATAAACTCCTGGAACAGTT *F TGTGCTCAATAAATACACGGGATTGGAGAAGCCAGAAATGCCTGAACTGGAGTATGTGGCCTTGTCGAGTGGTTCTCGCC *F TTCAACAGCCGCAGGTCTCGGCCAGCGCATCTAACTCCAATTCGGCCACCAGCATTCAGGCTGCTCTGCGGGCTGGAAAT *F GCAGCAACGACGGCCTCGGGCAGTGCGGTGTCCATGGATCAACGGGGCGATGCCTTGGCGGCAATCTCTCTGGGCAGCGG *F AAGTGCAGCCAGCGGTAGTATACCCTTTGCCGAGGATCCGATACTGGGAGCTATGGGCTCTCCGCGTCCCACATCGCCGG *F ACATGCTGGCGGGAGCCAATGCATCGGGAATACAATCGCAACCGACTGGTGGAGCCACATCCACGGTGAAAAGCCTGCGA *F TCCTGGTTTTTGCCCACTGCCAAACAGCAACCGTCTGCAGGCGCCGGAAATTTCGGTATGGCCGGAAGTGGCAGCACCAA *F CAATACCACCACCACGACAACAGCAAGCAACAATAGCAACAACTTGAACACCAACAACACAACAGCAACAACTACAACAA *F CAACTGGCACGAATAATCTTACCACAAATATCACCAAAAATTCCCCTAATTCTGAGGATACTAGCAATCAAGTCTCTGGC *F ATTTTACGCAGTCGTCGCGACAAGACAAAAACCAAAAAGGCTAAGAAAAAGAAAGACAATGAGGCCGCCGAGAACATACA *F GGAAGAACGCCTCACCAGTTTGGATCGAGGCAACAACACGCTGCTGGACTATGACGACCATGGGCGCAGCATGAAGACGC *F AGAATTCCAGCGGCAGTAGCGCTGGCGGTGGCTTGGCCGCCCTATCGGTGACCTCAGCTCAAGGAAGCGTTGATTCTGCA *F GGAGCAAGTAGCATGGGCGGAAGCGGAGGTGCGGCAGTGGCCAGTGGGAGCCTACCCAATGCAGGCGCCCTGACCAACTC *F GGCTTCGATCACAGCTCCGGGCGCCGCAAGTGGGACCGGAACTGGATATGAGGTCGATGAGGAGGGATTTAGCATACAGC *F CTGCTAAGGAAATAGCGTGGGAGGAGGGCCAGGATAAATCTGGCAGCTTCTATTCCGACTCGGATACGGATTCGGACAAT *F GACAAACAGGAACGTCGGATCCATGTCAGCATTAAGCCTTTGAAGAATGGTCAAGCGCCCATGTCGGCCAGCGTGGATGA *F GCTGCGTGCCACCGTGGAGAACATTTCGCTTTCCCCAACTGGAGTTTTTTCACATCACAGCCAGCAGTTGCAAGCAGCGA *F GAGGAGCTCCGGCGTCGCGTCAACAGTCGCCGGAAATATCGAATGCCTCCACTCCCACGGCCAGTGTGCATCCTTATGCC *F CCGCTGCAGAGTCCCACGCTGTCCAACAATAATAACGCAAACAACACGACCAACAGTCGTTATGCCGATCTGGGCGATAT *F CTTCTCAGAGTTGGGTGACATGAGTATGTCGGCGCCCTCATCGGCGACGCTGGGAAAACCTCCCGGAAGGCAAATACCAA *F CACCGACGTCAGCGTCAACAGGTGGAAGCATAGCCATACCACGTCCGCCATCACGACGCTCTGATATGATTGCCATTGGA *F CCGGCGGCAACAGGGACAGCGCCAGGTCGCGGCAGAATGTCCCCGGCACCGGTGACGGCCATGAATCGAGCCGAGAGCAT *F TGGCAGTCTGGAGTTCCGTACGGCTATCGGGGGAGTGGGATCCTCGCGAGGCCCCTCTCCACTTACAATCGGTATTTCGG *F ACACTATACCGCTGGCTGTGGCATTCCACGAAATCATACACGCATATTTTCGGGGCAGCGATGAGTCGCGCTGCCAGGTG *F AAAGTGTCCGGTGATATGATGCTATCATTCCCAGCTGGCATTGCCGGCCTTTTAGCCAATAATCCAAACCCAGCCAAGCT *F GGGTTTTCGCATTAAGCACGTTCAGAATCTGGAAAACCTGGTGCCTAACGGAAAACTGGTGCAAATAGATCGCCAGCAGT *F CGAGTTCTGTGAGCACGATGCTGGAGTTCAATATGGGTGCTTTAACGGCCCTGCTCCGAAGGCAGGCGGAGAATAACCCG *F ACTGCCTCCTACTTCAACGTGGACATACTCAAGTATCAGGTGCGCACGAAGCCGGGAGCCAGCTCGTGTCCCTTCCAACT *F TGTCAGCTACTGGAAGTGTGAGTCCAGCTACACGGCCCTTAAAGTGGACTACAAGTACAATAACCACGCCATGGCCAGTG *F CCTCGCCCCTTCTGAATGTCACGCTCTCGGTGCCCGTCAATGGATCTGTGCGCAATGTCCAGTCAAAGCCGCATTCTGCC *F TGGTTGGGCGAATCGAATCGTTTGGTGTGGAACTTTACGGACATCTCGCAGAATTCACAAGACGGCGGAGTGGGGACGCT *F GCGGGCACGCCTGGAGCTTAACGATGGTCCCTCTACACCAGCACTACTGGCCACTCAGTTCAACTGCGAGGGCACCACAC *F TTTCGGGCATCGAGTTTGAGCTCCAGGGCAGCGGCTATCGCTTGTCGCTGGTCAAACGGCGCTTCGTTTCTGGAAAATAT *F GTGTGCGAAGGCGATGGCATTCGCAGTGGAGCCACACCCACGCCTCCGTCGGTCGGGTCCACCAGTCCGTACTCGGCGAA *F GTCAAATTAGTTACTCCCCACTCAATAGACGGCAAATGGGAACGAAAAATGGCAATATGGCAATAGGCTCGTGAAAAACT *F AGAGAAATTTTTGTAACTAATCTTAAATATGAGACAACCACATATACACCCAAACCATATTGAATTAGGCACACGAATAG *F AGGAGCCTACATAGTTTTCTCTGAGCTTTTTATTATACCCCCCTTTCTTGATTTCGAAATGGACATCACAATGCGGAATG *F AAATTGACAAACTTTAAGATTTCCACAGATCGAAGCACGACAAGATTGAGGAAACTGGAAATTCTATCAATTCGCATTCC *F AACAGTTTAGATCCAAACGAAATCGAAAGTGGGACCTATTATGTATTTTTGTACACACAAAATTCTGTTTTTCACGCGAA *F ATGTTGAATCTGCCTGCGGGAGGTCCGAAATGCGGGCCTGCAAAATCTGGATGTATTATTTGTTATATATATACGTATGC *F GAGTATATATTATTAAAGTGTATTTATTATGTTTAACTGAATATGATGTAAACGAACGATATTGTATGTAGCTATGTAGA *F TATCTAAAAACAACACACCGAACGTTAATTTTATGTTCACTTTTGTACACACAACCAAATCCAAATGTATTAAATGTAAG *F ATACAAAAAAAAAAAAAAAAAA *F \------------------------------------------------------------------------------ *F -- *F >LD20919 *F GAATTTTTCGCTTCAAATCGACTGAAGTTTACACGGGCTAAAGTATTACAGAAGTTTGGCTCAAGCCATTACACAACATG *F CAATAAGTAAATACAGAGGAGCTAAAATGTCGCAAAAAGAATTGGCGGCTCTGGTTGCCACGAGAACGAGTCGCAAACGC *F ACCATATCCTCGACCTCATTCTCTATTTCCACTCGCACTGATCCAAAATCCATCATAGAGATACACATATTCAATGACTC *F CTTGCCCATAAAAAGAGCTTGCTGCGAAGTGGAGAATCCAACTTTAAAAGCGGAACAGCGAGTGCAGCAGGAAGAAGAGA *F GCCTGGGCCAAGTGCCGCCGCTGACCGAGGAAGAGCAGCAGCGGCACGATGAGTTCCGAAATAGTGGTTCGATATTCCTG *F CAGGATTTGCCCTGCTTTAAGCTGCAGCAGGCACAGCCGATAGCAGGATCATCACCCATTCCCAAGTGTCGGGAGTGCAG *F AAGAAGAAATTTGGCTACAACAGAGGGCGAACCCAGTTCAGTTAGCGACGTTTACTGCCGGTTCTACGAgTTCCGACGCC *F TTCAGTTCAATGAGAATGGAGAGCTGTGCGTGGTTGGCTTCCCAAATCCCTACAGCGAGCCCTCACCAGAGGACATCGCC *F ATTTGGCAGCCGGATAAAAATACACCGCCGACCAGCGGTTACATGGATATACAGGTTTGCAGGTACATCCTACTCCATGC *F CGGCGACCAGTTCTGCTATATTTGGCACCAGGAAGCAGAGGCTTTAAGTTTGCATCAGAATACGGACGGAACCATTGCCT *F GGAAGAAGGCCGTAAAAGGAACACGAGAGATTTGCGACGTGTGTGACACCACATTGTTCAACTACCATTGGACTTGCCGC *F AAGTGCGGCTTTGGAGTGTGTCTGGACTGCGTTAAAGATCGCAAGGAGGGATTGCGACTGCGGCGAGCGGAGAACGCTGC *F CCAAAAGGGATGCGATGAGTACCACTGGCTGCTCTGCAGCGACCCCAGTGGCCCACAGGAGCACGTCCTTACCGAGCTGA *F TGCTAACACAGATCATAGCCGGAGATGCCCTCAACGTCTTGGGCAGGCTGCTGCACGAGGTTCGTACTTTGTGGCAGGTG *F CCACAGGTCTGCGGCTGCCTTCTGAGCAAGCAAGCGATTGAAGATGCACAATCAAAGGAAGTGATCCAGGATATGATTAA *F GGAGTCTCAGCTGAAGCAGCACACCAGCTACTCGTCGCTGGCCTCCGAGCAGAAGGTGCACCAGCAACAGCGCCTCGATC *F AACTGCACGCCACGAAGTTGGAGTTTGCCAGAGAGCTAGGTGTAGATTATGTTCCAGGTCGAGTGTGGACCAAGGAAACC *F TTGGGAAAGGATCCAATAACAACGGCGTTTGATAATTTGAAGCACATTAATTTTCTGAGAAAGGGATTGGCCGGCTTGAG *F AAGGTTTCTTCCACCAAGGACCATGACTTTTGCTTACTCCACTCAGTTGGCTCCAGGAGTTCCTCACGAGTTTCTGTGCG *F ATGGCAGACTGCTTAGACTCACCGATGCCATGCATCCGGATAATCGAGTTCTTTTCCAGGAGGTTTGGAAATGCGGCCAG *F CCAGTTATGATTTCGGAGGTGGCTCGCTCTTTGAACTTAGACTTATGGCATCCACAAGCCTTTTGCCGGGACTTTGGCGA *F CAAGCCCAATGATCTTATTAATTGCCTGAATGGCAATCTGGTGCCCAATCAGCCAATGCGACACTTTTGGGAGGGTTTTC *F AGTGCATGACCAAGCGTCTGCCAGATGCATATGGCAAGCCAATGCTCTTAAAGCTTAAGGATTGGCCGCCGGGTGATGAT *F TTTGCCGAAATACTGCCCACCAGGTTTGCTGATTTGATGAAGGGACTGCCCATGCCGGAGTACACACTTCGCACGGGAAA *F CCTTAACATAGCTAGCTGCTTACCCAAGATGTTTGTGCCACCAGACTTAGGACCCAAAATGTACAACGCATATGGCTCAG *F CATTGCACCCCGACAAGGGTACAACCAATCTTCACTTGGACATTTCGGATGCAGTGAACATAATGGTTTATGTGGGAATA *F CCCCAGGATGGGGACACGAGACCTCAGATGGCGGCCACGCAGAAAGCCATAGAAATTGGCGGATGCGATTACATCACTAG *F AGCTCGTTGCCAATCGCCAGATGTATTACCCGGAGCACTTTGGCACATCTTTCCAGCTCGCGATGCAGACAAAATAAGAG *F ATTTACTTAACCGCGTGACCCTGGAGAAGGGTTTCCGTCTGGAGCCGGATCACGATCCAATTCACGATCAGAATTGGTAC *F TTGGACGACAAGTTGCGAGCTCGTTTATTCAAAGAGTATGGCGTGGAGGGGCATCCGATTGTCCAGTGCTTGGGCGACGC *F AGTCTTTATACCAGCTGGCGCCCCACATCAGGTTCAAAATCTCCACAACTGTATTAAGGTGGCCGAGGATTTTGTATCGC *F CGGAAAATATCACGCACTGCTATCATCTAACTCACGAGTTTAGAAGGCTTTCGCACTCGCACACAAATCACGAGGACAAG *F CTTCAGATCAAGAATATTATCTACCATGCCATCAAGGACTGTTGCACCATCCTGACCAGAGCTGTGGATGAGAGACTTAA *F TGCGGAATTAACCAAGCTAAATGCAGATTAGAATTTTCTTACCTTTTAGTTTTTTTCAACTATTTAACATTTATTATTAT *F TTTTTAAGATCAAAAATTGTCAAATATTCAATAAATAACTTACTTAGAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAA *F \------------------------------------------------------------------------------ *F -- # *U FBrf0149613 *a Saunders *b R.D. *t 2002.6.13 *T personal communication to FlyBase *u *F From: R.D.Saunders@open.ac.uk *F To: gm119@gen.cam.ac.uk *F Cc: lesley.mclellan@cancer.org.uk *F Subject: RE: FlyBase query: Glutamate-Cysteine ligase *F Date: Thu, 13 Jun 2002 15:10:49 \+0100 *F Hi Gillian, *F Happy to answer your query. Some enzyme commission renamed the enzyme *F subunits, so that: *F gamma Gltuamylcysteine synthetase, or gammaGCS became glutamate cysteine *F ligase, or GCL *F The two subunits formerly had the suffixes h for 'heavy' and l for 'light'. *F These now become GCLC (for GCL catalytic subunit), and GCLM (for GCL *F modifier subunit). *F So GCSh = GCLC and GCSl = GCLM. We prefixed these with Dm to indicate *F Drosophila melanogaster. *F Hope this clarifies the matter! .. *F Robert *F Dr Robert D C Saunders *F Lecturer in Molecular Biology *F Department of Biological Sciences *F The Open University *F Walton Hall *F Milton Keynes MK7 6AA *F Tel: 01908-654069 *F http://www.open.ac.uk/science/biosci/research/molecular-genetics/molgen.html *F > \-----Original Message----- *F > From: Gillian Millburn mailto:gm119@gen.cam.ac.uk *F > Sent: 13 June 2002 15:03 *F > To: r.d.saunders@open.ac.uk *F > Cc: gm119@gen.cam.ac.uk *F > Subject: FlyBase query: Glutamate-Cysteine ligase *F > *F > *F > Dear Dr. Saunders, *F > *F > I am curating your paper for FlyBase: *F > *F > Fraser et al., 2002, J. Biol. Chem. 277(2): 1158--1165 *F > *F > and I have a couple of questions. *F > *F > A. is the 'DmGCLM' gene in this paper, the same gene as that called *F > 'DmGCSl' in your abstract: *F > *F > Saunders et al., 2001, Europ. Dros. Res. Conf. 17: F34 *F > *F > 'Glutathione synthesis in Drosophila: molecular and genetic *F > characterisation of gamma-glutamylcysteine synthetase.' *F > *F > I am pretty sure it is the same gene but I just wanted to check. *F > *F > B. I'd like to sort out the nomenclature of the *F > 'gamma-glutamylcysteine *F > synthetase' genes in FlyBase. *F > *F > At the moment we have: *F > *F > 1. for the catalytic subunit: *F > *F > gene symbol: Gcsh *F > full name: gamma-Glutamylcysteine synthetase catalytic subunit *F > *F > this corresponds to CG2259 and is the gene that you called 'DmGCS' in *F > your paper Saunders and McLellan, 2000, FEBS Lett. 467(2-3): 337--340 *F > and 'DmGCSh' in your abstract Saunders et al., 2001, Europ. Dros. Res. *F > Conf. 17: F34. *F > *F > In your 2002 JBC paper you call this 'DmGCLC' for *F > 'glutamate-cysteine ligase catalytic subunit' *F > *F > *F > 2. for the regulatory subunit (assuming that I'm right in A. above and *F > 'DmGCLM' in your JBC paper is the same gene as 'DmGCSl' in your EDRC *F > abstract): *F > *F > gene symbol: Gcsl *F > full name: gamma-Glutamylcysteine synthetase regulatory subunit *F > *F > this corresponds to EST GH01757, which means that it is predicted gene *F > CG4919. *F > *F > In your 2002 JBC paper you call this 'DmGCLM' for *F > 'glutamate-cysteine ligase modifier subunit' *F > *F > *F > are these 2 symbols above the ones that you want to be the valid *F > symbols in FlyBase, or would you rather have the valid symbols be the *F > ones you use in the 2002 JBC paper, i.e. *F > *F > 1. for the catalytic subunit: *F > *F > symbol: Gclc (or Gcl-c) *F > full name: Glutamate-cysteine ligase catalytic subunit *F > *F > 2. for the regulatory subunit *F > *F > symbol: Gclm (or Gcl-m) *F > full name: Glutamate-cysteine ligase modifier subunit *F > *F > Since your group is the first to have published on these two genes *F > you can choose the final valid symbols for these genes in FlyBase. *F > Could you let me know what you would like the symbols to be (they *F > should be the symbols you intend to use in future publications), *F > *F > I look forward to hearing from you, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- *F > # *U FBrf0149614 *a Graham *b L. *t 2002.6.18 *T personal communication to FlyBase *u *F From: Sherry Gauthier *F To: cdsmith@bdgp.lbl.gov *F Subject: Drosophila odorant binding protein annotations *F Date: 18 Jun 2002 19:40:49 \-0400 *F Dear Dr. Smith *F Here are the files via our technician's e-mail account. *F Sincerely *F Laurie Graham *F \---- *F Hello *F We amplified cDNAs corresponding to 21 of the 38 Odorant binding protein *F homologues in *F Drosophila. This work is in press (Graham L.A. and Davies P.L. 2002 The *F odorant- *F binding proteins of Drosophila melanogaster: Annotation and characterization *F of a *F divergent gene family. Gene). I have given the coding sequences below with *F notes *F describing how our annotation compares with that done on the whole genome. *F Comparisons are *F also made with the annotations given by Galindo & Smith (Galindo K, Smith DP *F (2001) A *F large family of divergent Drosophila odorant-binding proteins expressed in *F gustatory and *F olfactory sensilla. Genetics 159(3):1059-72) or by Hugh Robertson via *F annotation updates. *F Our cDNAs were amplified from Canton-S so between 0-8 polymorphisms were *F observed per gene. *F The primers that we used to amplify the cDNAs are shown with each sequence. *F The 3' primer *F is shown in reverse complement so you can easily visualize its position relative *F to the cDNA sequence. The cDNA was derived from a mixture of all *F developmental stages *F (embryo-adult). All of the genes contain at least one intron which was absent *F in the cDNA *F sequence except Obp99d and Obp99b. Obp99b was independently confirmed by ESTs. *F OBPs typically contain 6 highly conserved Cys residues. However, some family *F members only *F contain 4 of these in which case Cys 2 and 5 are not present. Others contain *F insertions or *F deletions. These differences are noted below. *F In the cases where a gene name has not been given by Galindo & Smith, we have *F noted a *F compatible gene name below (Obp followed by the band number with an alphabetic *F suffix ex. *F Obp23a). *F > CG13517 Original gene prediction is fine. New name proposed...Obp59a. My *F coding *F > sequence shown below. Amplified from first strand cDNA using the following *F primers. *F > 5' end CACGGATCCCAAGATGAAACAGTTGAT 3' end GCTCTTCAATTAGGATTAAACTCGAGAGG. *F > This isoform contains two insertions (60 a.a. Gly-rich insert \+ 15 a.a. *F insert) each *F > containing a Cys residue (for 8 Cys in total). *F ATGAAACAGTTGATTTTCCTGCTGATTTGCTTGAGCTGCGGCACCTGCTC *F CATTTACGCACTGAAATGCAGATCCCAGGAGGGACTAAGTGAAGCGGAACTCAAGCGAAC *F TGTGCGCAACTGTATGCATCGCCAGGACGAGGACGAAGATCGAGGACGAGGTGGACAGGG *F CCGGCAAGGAAATGGCTATGAGTACGGTTACGGAATGGATCAGGATCAGGAGGAGCAGGA *F CAGGAATCCAGGCAACAGGGGCGGCTATGGCAATCGAAGGCAGCGAGGACTAAGGCAATC *F GGATGGCAGGAACCACACCAGCAACGATGGAGGTCAGTGTGTGGCCCAGTGCTTTTTCGA *F GGAGATGAATATGGTGGATGGCAATGGGATGCCCGATCGGCGCAAGGTGAGCTATTTGCT *F GACCAAGGACCTTCGGGACCGGGAGCTGCGCAACTTCTTCACGGACACCGTGCAGCAGTG *F CTTCCGCTATCTGGAGAGCAACGGAAGGGGCCGGCACCACAAGTGTTCAGCGGCCCGGGA *F ACTGGTCAAGTGCATGTCGGAGTACGCGAAGGCGCAGTGCGAGGATTGGGAGGAGCACGG *F CAACATGCTCTTCAATTAG *F > CG15883 Obp18a Original gene prediction incorrectly identified the first *F exon. Our cDNA *F > is compatible with the prediction made by Hugh Robertson in his annotation *F updates. The *F > sequence predicted in Galindo & Smith 2001 has 3 extra a.a. that we did not *F observe. *F > 5' end catgaaggttgtgtgcag 3' end ctatcgcctcgtaatttacctcgaggtg. Also *F confirmed by ESTs *F > (see BI243891. RE41704.5prime RE...gi:14712817). *F ATGAAGGTTGTGTGCAGCATAGCTGTACTATGGATTTGCTTGATAACTATGTGG *F CAATCAGCTGGCCGCGTTAACGCAGAGGGTTGCCTAAAGCACCACAATCTGACCAGTGCC *F CAAGTGCAGGCAGTGGCTCCATCCACTCCCGTTGCGGATGTTCCAGTGGCCGTTAAGTGC *F TATAGCCGGTGTCTGATCCAGGATTATTTCGGTGATGATGGGAAAATCGATCTGCAGAAG *F GTGGGAAAGCGAGGATCTCAAGAGGACCACGTGATTTTGTCCCAGTGTAAGCAGCAGTTC *F GATGGCGTCACCAATCTGGACACGTGCGACTATCCATACCTAATTCTCCAGTGTTATTTT *F AAGGGCAAGCAGAGTGGAACTATCGCCTCGTAA *F > CG15457 Obp19c Original gene prediction is fine. *F > 5' end GAAGGATCCAAGATGAAGCCATCCACT 3' end GTCTGGTAGAGGACATCTCGAGGTG *F ATGAAGCCATCCACTCCAGTCGCAGCCATTCCGCTAATGACGATAGTGGTC *F GCTGTCCTGCTGCAGACGCACTGCGTCCGTGGCCAGACCCAGGCTTTCGATCTCGCCAAG *F CTGCTGCCCAAGACCGGAACGGAGCCCATCTGGGCGGTAATCGATCGCAACTTGCCGCAG *F GTGCAGGAGCTGGTCACCGCGGCCAGGATGGAGTGCATCCAGAAGCTGCAGCTGCCCAGG *F GACCAGCGACCGCTGGGGAAGGTGACCAATCCAAGTGAGAAGGAGAAGTGCCTGGTGGAG *F TGCGTGCTCAAGAAGATCAAGTTGATGGACGCCGACAACAAGCTGAACGTTGGCCAGGTG *F GAGAAGCTGACCAGCCTGGTGACCCAGGACAACAAGATGGCCATCGCCGTTAGCTCCAGC *F ATGGCGCAGGCCTGCAGCCGCGGCATCTCCTCGAAGAACCCCTGCGAGGTGGCCCACCTC *F TTCAACCAGTGCATCAGTCGCCAGCTGGAACGCAACAACGTAAAGCTGGTCTGGTAG *F > CG11748 Obp19a I agree with Hugh Robertson that the initiator Met is *F closer than *F > originally predicted. There is a high scoring TATA-containing promoter well *F > positioned relative to this later Met. The second intron proposed by *F Galindo & Smith *F > is longer than the intron we observed. *F > 5' end gaaggatccggaaatgaagttccatctg 3'end gtattcgtgtttccctaggctcgaggtg *F ATGAAGTTCCATCTGCTGCTGGTCTGCGTCGCCATATCCCTGGGCCCAAT *F CCCCCAGTCGGAGGCAGGGGTGACGGAGGAGCAGATGTGGTCTGCCGGAAAGCTGATGCG *F CGATGTCTGCCTGCCCAAGTATCCGAAGGTCAGCGTCGAGGTGGCCGACAACATTCGCAA *F CGGTGACATACCCAATAGCAAGGACACCAACTGCTACATCAATTGCATCCTGGAAATGAT *F GCAGGCAATCAAGAAGGGAAAGTTCCAGCTGGAGTCGACCCTCAAGCAGATGGACATCAT *F GCTGCCGGACAGCTACAAGGACGAGTACCGCAAGGGCATCAATCTGTGCAAGGACTCCAC *F CGTCGGCCTGAAGAACGCCCCCAACTGCGATCCCGCCCACGCCCTGCTCAGCTGCCTGAA *F GAACAACATCAAGGTATTCGTGTTTCCCTAG *F > CG15583 Obp83g This region contains three OBP motifs that were originally *F predicted to *F > be fused into one construct. I agree with Hugh Robertson that the first *F motif forms a *F > separate gene. One codon was absent in our Canton-S construct. We did *F check for *F > alternative splicing to the other motifs using PCR but this was the only *F product *F > recovered. 5'-end gaaggatccagaaatgcagtcccaatc 3'-end *F ggtcttcgcctgacctgaatctcgagagg. *F > EST data supports this gene structure (see BI229555.RE27361.5prime *F RE...gi:14696819). *F ATGCAGTCCCAATCCCTCCTGCTGATCGCAGCCGTTGCCACGTTTCT *F GGTGGCCCAGACTACGGCCAAGTTCCGGCTGAAGGACCACGCCGACGCAGAGAAGGCGTT *F CGAGGAGTGCCGTGAGGACTACTACGTGCCGGACGACATCTACGAGAAGTACCTGAACTA *F CGAGTTTCCCGCCCACCGGCGCACCAGCTGCTTCGTCAAGTGCTTCCTGGAGAAGCTTGA *F GCTGTTCTCGGAGAAGAAGGGATTTGACGAGCGCGCCATGATCGCCCAGTTCACCTCCAA *F GAGCAGCAAAGACCTGTCCACGGTCCAGCACGGCCTGGAGAAGTGCATCGACCACAACGA *F GGCCGAGTCGGATGTCTGCACCTGGGCCAACCGAGTCTTCTCCTGCTGGCTGCCCATCAA *F CCGCCACGTGGTGCGTAAGGTCTTCGCCTGA *F > CG15583 Obp83f (Obp83e). This is the second portion of the original *F prediction *F > containing two OBP motifs. These motifs are not separated by an intron. *F Galindo & *F > Smith predicted a monomer at this location and Hugh Robertson suggested *F either a monomer *F > or a dimer and raised the possibility of alternative splicing. A cDNA *F containing a *F > single motif could be produced by removal of an intron which would introduce *F a stop codon *F > to the end of the first motif. However, the PCR products obtained were not *F spliced at *F > this location. Rather, the cDNA contains two fused OBP motifs (Obp83f \+ *F Obp83e) and is *F > an 'obligate heterodimer'. 5'-end CACGGATCCGCAGAGAGATGAGTTCC *F > 3'-end GAGGACAACGAAGAGCAGTAGAAGCTTGTC. To check for alternative *F splicing..used internal *F > primer ctgtcgacta agtgggactcgagagg in combination with primer at 5' end. *F Also used 5' *F > primer of Obp83g in combination with these two 3' primers and did not *F recover a product, *F > indicating that this locus does encode two separate genes (monomer and an *F obligate *F > heterodimer). No evidence of alternative splicing. *F ATGAGTTCCTCTCGTGCGGTTCTAGTCAGCCTGTTCCTGATCTGCAGTCAGGCACTAGCT *F GACCTTTCTGGTGATGCCCAGACTCTGGAAAAGTGCCTGCGGGAACTTAGTTCGCCGGAG *F AGCATTGCTGGCGATCTTCGAAAGCTGGAACGGTATTCATCTTGGACGCGGGAGGAGGTA *F CCTTGCCTGATGCGCTGCTTGGCCAGAGAAAAGGGCTGGTTCGACGTGGAGGAAAACAAG *F TGGAGGCTCAAGCAACTGACTGAGGACCTGGGCGCCGATGTCTATAACTACTGCAGATTC *F GAGCTGCGCCGGATGGGGTCCGATGGCTGCAGCTTCGCCTATCGGGGACTCAGGTGCCTG *F AAGCAGGCCGAGATGCATGCGGGCACCAGCCTGAGCACACTGCTACAGTGTTCCCGCCAG *F CTGAACGCCACCAACGTGGAGCTGCTGCAGTACAGTAAGCTGAAGTCAAAGGAACCTATT *F CCCTGCCTCTTTCAGTGCTTTGCGGATGCCATGGGATTCTACGATCCCGATGGAAACTGG *F CGGCTGGAAAACTGGAAGCAGGCGTTTGGGCCTTCCGGAAATGAGGATCAGTCTTCTGGC *F GCTGACTACAGTGGCTGTCGACTAAGTGGGACTCAGCGGGAGGTGGCGCTAAGCAAGTGC *F TCGTGGATGTACCATGAGTACAAATGCTGGGAGCGAGTAAATGGGAATAAGCTAGTGGAG *F GACAACGAAGAGCAGTAG *F > CG15582 Obp83c/d This was originally annotated as a fusion of two OBP *F motifs but the 5' *F > exon was not predicted correctly and did not encode a signal peptide. *F Galindo & Smith *F > predicted two separate genes and Hugh Robertson raised the possibility of *F alternative *F > splicing or a heterodimer. I agree with Robertson that there is no suitable *F signal *F > peptide for the second half of the gene. If the intron separating the two *F motifs was *F > not removed, a monomer would be produced. Alternatively, the second motif *F could be *F > spliced to the signal peptide encoding exon upstream of the first motif. *F Using primers *F > to test each possibility, we only recovered a cDNA encoding the obligated *F heterodimer *F > and found no evidence of alternative splicing. In addition, although the *F portion of the *F > ESTs sequenced does not include the entire coding sequence, it is long *F enough to confirm *F > the heterodimeric structure (see gi|15319558|gb|BI485590.1|BI485590). *F > 5'end-GAAGGATCCGCAATGCAGATGAAAAGTG GA 3' end-CTACG CGATGGCTGTTTAGCTCGAGGTG *F ATGCAGATGAAAAGTGGAATATTAATAGCATTATGTCTATGCCTTTCGTTG *F AACGAAGGCCTGGCCCTTCTGGAGCACGAAGGCGAGACCATCAACAGATGCATCCAAAAC *F TATGGCGGACTTACTGCGGAAAATGCCGAACGTCTAGAACGATTCAAGGAATGGTCGGAT *F AGCTACGAGGAAATCCCCTGCTTCACGCGCTGCTATTTGTCCGAGATGTTCGACTTTTAC *F AATAACTTAACGGGCTTCAATAAGGACGGAATTGTGGGCGTCTTTGGAAGACCCGTCTAC *F GAAGCCTGCCGAAAGAAATTGGAACTGCCATTCGAATCAGGCGAGAGCAGCTGCAAACAT *F GCCTACGAGGGCTTCCACTGCATCACCAACATGGAAAGCCACCCGTTCACGGTTATTGAC *F AACATGCCAAACATATCCCCGTCGGCCAAGGATGCAATGAAGGACTGCCTGCAGGATGTC *F CACCAGGACGAGTGGAAGAGCTTCGATGCCTTCGCCTACTATCCTGTCAATGAACCGATT *F CCGTGCTTCACCCGGTGCTTCGTGGACAAGCTGCATATCTTCGAGGAGAAAACGCGTCTT *F TGGAAACTGGAGGCGATGAAGCAAAACCTGGGCATTCCGGCCAAAGGAGCTCGCATAAGG *F ACCTGCCATCGGCACCGCGGCAGGGACCGATGTGCCACATATTACAAACAGTTCACCTGC *F TACGCGATGGCTGTTTAG *F > CG155505 New name proposed...Obp99d. This is a four-Cys isoform. The *F original gene *F > prediction is fine. This OBP has a small internal deletion of about 17 a.a. *F relative *F > to other OBPs. *F > 5' end-gaaggatccgcaatgaatca cttgagac 3'end-ggaagtggaa cgatgaaatactcgaggtg *F ATGAATCACTTGAGACTGGAGATCATCTGCTGGAGCTGCCTGCTTATTGCG *F ATGGCTGTTATCACGGAAGCTGCTTCTGTTTGGAAACTACCCACCGCGCAGATGGTCTAC *F GAGGATCTGGAGAAGTGTCGCCAAGAAAGCCAAGAAGAGGATGCTGCTACCCTGAGGTGT *F TTGGTTAAGAAACTGGGTCTTTGGACGGATGAGAGTGGCTACAATGCCAGGCGGATAGCA *F AAGATCTTTGCCGGACACAACCAGATGGAGGAGCTGATGCTGGTGGTGGAGCACTGCAAC *F CGGATGGAGCAGGACACGAGCCACCTGGACGACTGGGCCTTCCTGGCCTACAGGTGCGCC *F ACTTCCGGGCAGTTTGGCCATTGGGTCAAGGACTTCATGAGTCAAAAGGAAGTGGAACGA *F TGA *F > CG13429 Obp57e. I agree with Hugh Roberston and Galindo & Smith that the 5' *F exon was *F > missed and the 3' exon does not exist as indicated in the original prediction. *F > 5' end-GCTGGATCCGTATGTTGGACCAACTTACAC 3' end-GTTTCAATTAGTAATGCAAAGTAGCTCGAGAGG *F ATGTTGGACCAACTTACACTGTGTTTGTTGCTAAATTTTCTGTGCGCAAATG *F TTCTCGCTAACACTTCAGTATTTAATCCGTGTGTTTCGCAAAATGAGTTATCCGAATATG *F AAGCCCACCAAGTGATGGAGAATTGGCCAGTTCCGCCCATCGATCGGGCTTACAAATGCT *F TTCTAACTTGCGTCCTTTTGGATTTGGGCCTGATTGATGAACGGGGTAATGTGCAGATCG *F ATAAGTACATGAAATCCGGAGTGGTGGACTGGCAATGGGTGGCAATAGAGTTGGTAACAT *F GTCGCATAGAATTCAGCGACGAAAGGGATCTGTGCGAGCTATCATATGGAATCTTCAACT *F GCTTCAAGGATGTGAAGCTTGCGGCCGAGAAGTATGTTTCAATTAGTAATGCAAAGTAG *F > CG13874 Obp56h. I agree with Galindo and Smith & Robertson that the 5' *F signal-peptide *F > encoding exon was missed. 5' end-gagggatcctcaaaatgaagttcaccct, *F > 3'end-acatcactaatgcctgatcctcgaggtg *F ATGAAGTTCACCCTATTCTGTATTGCTCTGGCAGCTTTTTTGTCCATGG *F GACAGTGTAATCCGGACTTTCGCCAAATAATGCAACAGTGCATGGAGACCAACCAAGTGA *F CCGAGGCTGATCTCAAGGAGTTCATGGCCAGCGGGATGCAGAGCAGTGCCAAGGAGAACC *F TCAAGTGCTACACCAAGTGCCTGATGGAGAAGCAGGGTCATCTCACCAATGGCCAGTTCA *F ATGCACAGGCTATGCTCGACACTCTCAAAAATGTGCCTCAGATCAAGGACAAAATGGACG *F AGATTTCCTCGGGAGTGAATGCCTGCAAGGACATCAAGGGAACCAACGATTGCGACACGG *F CCTTTAAGGTTACCATGTGCCTGAAGGAGCACAAGGCCATTCCAGGACATCACTAA *F > CG13873 Obp56g. I agree with Galindo & Smith and Robertson that the 5' *F signal-peptide *F > encoding exon was missed. Two splice donor sites are predicted 3 bases apart. *F > Our transcript was spliced at the earlier (somewhat lower scoring) site, so *F the protein *F > encoded by our cDNA is one a.a. longer than that predicted by Hugh Robertson. *F > 5'end-gtcggatccatgagggctacattcgcattg 3'end-caggcagccaattagggtctctcgagtcc *F ATGAGGGCTACATTCGCATTGACTCTGTTGCTCGGCTGCCTTTCAGGAATTTTG *F GCGCAGCAAGCCAACATAGACAGTTCGGTGTCCAAGGAACTGGTGACGGATTGCCTCAAG *F GAGAACGGTGTCACTCCCCAGGATCTGGCTGACTTGCAATCGGGCAAGGTGAAGGCCGAG *F GATGCCAAGGACAATGTGAAGTGCTCCTCACAGTGCATTCTGGTCAAGAGCGGTTTCATG *F GACTCCACTGGCAAACTGCTGACCGACAAGATTAAGTCTTACTATGCGAACTCGAACTTT *F AAGGATGTCATCGAAAAGGATTTGGACAGGTGTAGCGCGGTCAAGGGGGCCAATGCCTGT *F GACACTGCCTTCAAGATATTATCCTGCTTCCAGGCAGCCAATTAG *F > FBgn0043532 Obp56i. I agree with both Robertson and Galindo & Smith that *F there is a gene *F > here (not originally predicted) and our cDNA matches their predictions. I *F have submitted *F > the cDNA sequence to genbank (AF457143) and release was requested on May *F 30th (still *F > pending). 5'end-GCTGGATCCTACCCTGCTGAAAAATGCAT *F 3'end-CGAAATCACAGAGGATAAAGTCTCGAGTCC *F ATGCATTTTTTCGCCTGCTGTGCATTATTGTTAGTCGTCG *F TTACTTTACCAACATGTTTCGTACAAGCAGGTCCCATTAAGGATCAATGCATGGCGGCGG *F CGGGCATCACAGCACAAGATGTTGCGAATCGTCATGAGACCGACGACCCTGGCCATAGTG *F TCAAGTGCTTTTTCCGCTGTTTTCTAGAAAACATTGGCATTATCGCCGATAACCAGATAA *F TACCCGGTGCTTTTGACCGAGTTCTAGGCCATATAGTTACCGCGGAAGCCGTAGAGCGAA *F TGGAAGCGACGTGTAATATGATTAAGAGCGAGACAACCTATGACGAGTCTTGTGAATTCG *F CCTGGCAAATCTCCGAGTGCTACGAAGGAGTAAGATTATCAGATGTTAAGAAGGGCCAAA *F GAACTCGAAATCACAGAGGATAA *F > FBgn0043536 Obp57d. I agree with both Robertson and Galindo & Smith that *F there is a gene *F > here (not originally predicted) and our cDNA matches their predictions. I *F have submitted *F > the cDNA sequence to genbank (AF457149) and release was requested on May *F 30th (still *F > pending). There is some uncertainty as to the initiator Met. The others *F have predicted *F > a start at the second ATG in this sequence. Our primer location does not *F unequivocally *F > differentiate between the two as is contains 15 nt which lie between these *F two ATGs. *F > 5'end-AGCGGATCCTATGATATCTTCAACTAGT 3'end-GTCATAAAAGCCGTTGTAAAGACTCGAGCCT *F ATGATATCTTCAACTAGTTTGATGCCTGAAAAAATGTCTCTAAGACTCATACC *F GCATCTGGCTTGTATTATTTTTATTTTGGAAATTCAGTTTAGAATTGCCGATTCTAACGA *F TCCGTGCCCCCATAATCAAGGAATAGACGAAGATATAGCCGAATCAATTCTAGGTGACTG *F GCCTGCAAATGTGGATTTGATTAGCGTGAAAAGGTCCCACAAGTGTTATGTGACCTGCAT *F TTTGCAATATTACAATATTGTGACCACTTCTGGTGAGATATTTCTGAACAAATACTACGA *F TACTGGAGTCATTGATGAATTGGCGGTGGCACCCAAAATCAATCGATGCCGATATGAGTT *F TAGAATGGAAACAGATTATTGTAGCCGAATTTTTGCTATATTCAATTGTTTAAGGCAAGA *F AATATTAACAAAGTCATAA *F > FBgn0043534 Obp57b. I agree with both Robertson and Galindo & Smith that *F there is a gene *F > here (not originally predicted) and our cDNA matches their predictions. I *F have submitted *F > the cDNA sequence to genbank (AF457147) and release was requested on May *F 30th (still *F > pending). 5'end-actggatcctacaatgttcatctacagac *F 3'end-cgtaattgattcggaataaatgctcgagagg *F ATGTTCATCTACAGACTTGTATTTATTGCGCCTCTGATTTTGTTATTGTT *F CAGCTTGGCCAAGGCTCGCCACCCCTTTGATATATTTCATTGGAATTGGCAAGACTTTCA *F GGAGTGTCTACAAGTTAATAATATTACCATAGGAGAATATGAGAAATACGCGCGACACGA *F AACTTTGGATTACCTGCTCAACGAGAAAGTCGACTTGAGGTACAAGTGCAATATTAAATG *F TCAGCTGGAAAGGGATTCAACGAAATGGTTGAATGCTCAAGGCAGAATGGATTTGGATTT *F GATGAATACCACCGATAAGGCATCCAAATCCATTACCAAGTGCATGGAGAAGGCTCCCGA *F AGAACTTTGTGCGTACAGTTTTAGACTGGTGATGTGTGCATTTAAGGCCGGCCATCCGGT *F AATTGATTCGGAATAA *F > FBgn0043535 Obp57a. I agree with both Robertson and Galindo & Smith that *F there is a gene *F > here (not originally predicted) and our cDNA matches their predictions. I *F have submitted *F > the cDNA sequence to genbank (AF457148) and release was requested on May *F 30th (still *F > pending). 5'end-actggatccaatgttcaacactagacttgc *F 3'end-gattacgataccatcgatttataactcgagagg *F ATGTTCAACACTA *F GACTTGCCATTTTTTTGCTTCTTATCGTTGTTTCGCTTAGCCAAGCTAAGGAAAGCCAAC *F CCTTTGACTTTTTCGAAGGAACCTATGACGATTTTATTGATTGTCTGAGAATCAATAATA *F TTACCATTGAAGAGTATGAGAAGTTTGACGATACCGACAATTTGGATAATGTCCTCAAGG *F AAAATGTCGAACTGAAGCACAAGTGCAACATTAAGTGTCAACTGGAAAGAGAGCCAACCA *F AATGGCTAAATGCTCGGGGTGAAGTCGATCTGAAATCAATGAAAGCAACCAGTGAGACAG *F CGGTATCCATATCAAAGTGCATGGAGAAGGCTCCCCAAGAAACCTGTGCCTACGTCTATA *F AATTGGTAATATGTGCATTCAAATCCGGACATTCAGTCATCAAGTTCGATTCATATGAAC *F AAATACAAGAGGAAACCGCTGGACTAATAGCTGAACAGCAGGCGGATCTGTTTGATTACG *F ATACCATCGATTTATAA *F > FBgn0043533 Obp56f. I agree with both Robertson and Galindo & Smith that *F there is a gene *F > here (not originally predicted) and our cDNA matches their predictions. I *F have submitted *F > the cDNA sequence to genbank (AF457146) and release was requested on May *F 30th (still *F > pending). 5'end-actggatcctatgaaagtattcctgttgttc *F 3'end-CCATTACCAAGCATTAGACTTCTCGAGACT *F ATGAAAGTATTCCTGTTGTTCATTTTCATCTCTGCTATCTGGCTCCAAGCATTTTGTATG *F AAATCTTCTGAAAAAATAAAAGCCTGCTTGAAACGGCAGCTGGGGTATA *F CAATTACAGAAAATACAAAATTTGATGCTAAAGAAGACTCTCTTCAAAGCAAGTGTTTTT *F ATCACTGCTTACTGGAAGTGAAAGGTGTTATTGCAAATGATGCGATCAGTTCGGAGCAAC *F CGAGGAAAGTACTTGAAAAAAAGTATGGCATTACTGACACAGATGAATTGGAAAAGGCTG *F AAGAAAAGTGTCATTCCATCAAGGCTTCAGGAAAATGTGAATTGGGCTACGAAATCTTGA *F AATGCTATCAGTCCATTACCAAGCATTAG *F > CG12944 Obp47a. I agree with Robertson and Galindo & Smith that the *F initiator Met is *F > earlier than originally predicted. However, I agree with the original *F prediction of the *F > splice acceptor site which lies 15 nt further downstream than that predicted *F by Robertson *F > and Galindo & Smith. However, there were 7 polymorphisms relative to the *F Celera *F > sequence, so we amplified the genomic sequence as well. The polymorphisms *F were confirmed *F > and the intron sequence had numerous additional polymorphisms which could *F potentially *F > result in use of an earlier junction (end of lowercase on bottom line) in *F the strain *F > sequenced by Celera. This is the intron alignment. *F > Canton-S (Mine) *F gtgagtttgggttcaaaaaacaag----caaagggatttgcttataaaagcacctcttttcttcag *F > ::::::::::::: :::::: :: ::: ::::::::::::::::::::: :::: *F : :::: *F > Celera *F gtgagtttgggttaaaaaaaaaaaaaaacaatgggatttgcttataaaagCACATCTTCT-GTCAG *F > 5'end-GAGTCTAGAAATGAATCGAGTTCTAGTGC 3'end-CTTAGAACTTTGCACAACACTCGAGGTG *F ATGAATCGAGTTCTAGTGCTATTGCTGGTGCTTAAAATGTTCGCTTTGAGCGAGTCCCGT *F TTCGCCAAGATAAACATCAATCTGGGACTAACCGTTGCTGATGAATCCCCCAAAACGATC *F ACCGAGGAAATGATTCGCCTGTGCGGAGATCAAACGGATATATCCCTCAGGGAGTTGCAC *F AAGCTGCAAAGGGAGGACTTTTCGGATCCCTCGGAATCCGTCCAGTGTTTCACCCATTGC *F CTCTACGAGCAAATGGGTCTCATGCACGATGGTGTTTTTGTGGAACGCGATCTATTCGGG *F CTTCTTTCCGATGTCAGTAATCCCGATTACTGGCCAGAACGTCAATGCCACGCGATTCGT *F GGCAATAACAAATGTGAGACGGCCTACAGGATTCATCAATGCCAACAGCAGTTGAAACAA *F CAGCAAAAGAACTTATTGGCCACCAAGGAGGTTGAGGTCACCACCACACCAGCTGGATCC *F GATGAAACAAAACCTTAG *F > FBgn0043530 Obp51a. I agree with both Robertson and Galindo & Smith that *F there is a gene *F > here (not originally predicted) and our cDNA matches their predictions. I *F have submitted *F > the cDNA sequence to genbank (AF457145) and release was requested on May *F 30th (still *F > pending). 5'end-atcggatccagtattcattggcctggttc *F 3'end-cttgtaaaattgtatctgaacctcgagagg *F ATGAAAGTATTCATTGGCCTGGTTCTGTTGTTAGCTGTCACTACGCTGTCATCCGCTTT *F ATTCGAATCTGAAGCGAACGAATGTGCTAAAAAGCTGGGAATTACCCCAGATTACTTCGA *F AAATTTTCCGCACAGCAGTCGGGTGAAGTGCTTTTACCACTGCCAAATGGAAAAACTTGA *F AATAATTGCCAATGGTGTGGTAACACCATTCGATTTGAAAGTATTGAACATATCACCGGA *F GAGCTATGATAAGTATGGTGTAAAGGTAAAACCATGCCTCAAACTATCGCATCGCGACAA *F ATGTGAGCTCGGTTACTTGGTGTTCCAGTGCTTGAAACGAGAATTCAACTTGTAA *F > CG15129 Obp56b. I agree with both Robertson and Galindo & Smith that the *F original *F > prediction has fused two separate OBP genes. I was not able to amplify a cDNA *F > corresponding to the prediction although I did amplify the cDNA for each OBP *F > individually (second one below). My cDNA matches the prediction of *F Robertson and *F > Galindo & Smith. *F > 5'end-GAGGGATCCGAATGTAGCTTTGGAAAGATG 3'end-GTTAAGGGTATTAGTGCCTAACTCGAGGTG *F ATGAAACTTATCTACTTGTTGGTTGTATTCCTAATT *F TTCGCTCTAAGCGAACTAGTAGCGGGCCAGTCAGCTGCGGAATTGGCAGCCTACAAGCAA *F ATTCAACAAGCCTGCATCAAGGAGCTGAATATTGCTGCCAGTGATGCTAATTTGCTGACC *F ACCGACAAGGAGGTGGCGAATCCCTCTGAGTCGGTGAAGTGCTATCACAGCTGCGTCTAC *F AAGAAACTGGGTCTCCTGGGTGACGATGGAAAGCCCAATACTGATAAGATCGTTAAGTTG *F GCCCAGATCCGTTTCAGCAGTCTGCCGGTGGATAAGCTAAAGAGTTTGCTTACCAGCTGC *F GGAACCACAAAGTCAGCCGCCACCTGTGACTTTGTCTACAACTATGAAAAGTGTGTTGTT *F AAGGGTATTAGTGCCTAA *F > CG15129 Obp56c. Second OBP in fused prediction. Differed from predictions *F of Robertson *F > and Galindo & Smith in that we did not observe a second intron. Therefore, *F in addition *F > to having longer than usual N and C termini, this isoform also contains an *F insertion of *F > about 40 a.a.. Galindo & Smith also predicted a different 5' signal peptide. *F Original *F > gene prediction, although fused upstream of Obp56b, includes the observed *F signal peptide *F > (predicted start codon 21 nt downstream of actual start) but not the second *F intron. *F > 5'end-GAGGGATCCTTAGATGTATTTTCGAGCCAG 3'end-GCTAAGTCGGAGTAAATAGCTCGAGGTG *F ATGTATTTTCGAGCCAGTTTGATGGCATTGCTTTGCCTCACTCTTAGTGAATTCGTTTCT *F AAAGCATGGACCCGATCGCTTTCCGTCTCGCTGAACATGTCGATGACACGAACCCTGGTT *F CCAGATCCGCTAAATGGAACAGAAAACAAACTCAGCCAGGAGATGCTGAGGGCTTGTATG *F CGTAGGACCGAGATCTCAATGTCGCAACTGAAACTATTTCACATGAGCCTGATGAACAGC *F GACTACAATAATGACAACGATATAGCCCCTACGCCAGTTCAATCCATTGGCGATGTAAAT *F AACCTGGGTGATCTGGACTTCAATGGCAACTCGCAGATGCCCTATCTCGATCTGAAGCAT *F AATGAGCCGCTGCAGTGCTTTGTGAGCTGTCTGTATGAGACCCTGGATTTGGATAGGTAC *F AATGTCCTGCTGGAGGAGGCCTTTAAGAATCAGGTGCAAACGATCATACAGCATGAGAAG *F GCGGAGATCAAGGAGTGTAGTGATCTTCAGGGCAAAACACGATGCGAGGCAGCCTACAAG *F CTGCACCTGTGCTACAATCACCTGAAAACTCTGGAGGCGGAGCAGCGTATCCGTGAGATA *F CTTGAGCGGACCGAGGCGGAGAACGAAGGATTCGGTCCGGAGGGCAGCGACTTTATCGAC *F GGCATCCAGCATTCCGGAGAAGCAATGACCACCGCTAAGTCGGAGTAA *F > FBgn0043539 Obp22a. I agree with both Robertson and Galindo & Smith that *F there is a *F > gene here (not originally predicted). Our cDNA did not contain the second *F intron that *F > they predicted. We designed three primers to test three possible scenarios; *F that there *F > is no intron at the 3' end, that there is a short intron as predicted by the *F others, *F > or that there is a longer intron. All three possibilities introduce only a *F small number *F > of a.a. to the C terminus of the protein, so the actual transcript was *F difficult to *F > predict. We only obtained the cDNA corresponding to the no 3' intron *F version. I have *F > submitted the cDNA sequence to genbank (AF457144) and release was requested *F on May 30th *F > (still pending). This isoform has a small internal deletion of about 12 *F a.a. relative *F > to other OBPs. *F > 5'end-actggatccttcgagatgcgagtgttgct 3'end-ggatagatagaggatagagtctcgagcgt *F ATGCGAGTGTTGCTGGCTTTTGTACTTCTGCTTGGCCTCTCAGTTTTG *F GCCACTAAGGAACCGGAAGAAGTTAAAATTGTAAGCGAGTGTGCCAAGGAGAACAATGTT *F CATAGGAAGAAGGCACTGGACCTTTTAATGAGCTATCGTTTGAAGAAGAAAACCCACAAC *F GTCATGTGCTTCATCAACTGCATCTTCGAGCGAACCAACATACTGCAGAAAGTTAAGGAA *F AAGGTTGTAAAGGAAAATCACAACTGCGACTCCATCAAGGACGCTGATAAGTGTGCAGAA *F TCCTTCCAAAAATTTCAATGCTTGGTCAAGATTGAGATGAAAGTGAGGGGGATAGATAGA *F GGATAG *F > CG11797 Obp56a. There are 18 ESTs (several which appear not to be the same *F > clone resequenced) which confirm the original gene prediction. *F > See gi|15481152|gb|BI589730.1|BI589730 for example. *F > CG13421 Obp57c. There are 2 ESTs which confirm the original gene prediction. *F > gi|15523123|gb|BI627598.1|BI627598 *F > CG2297 We propose the name Obp44a. This is a four-Cys isoform. There are 87 *F ESTs which *F > confirm the original gene prediction. gi|15531140|gb|BI628930.1|BI628930 *F > CG11218 Obp56d. There are 24 ESTs which confirm the original gene prediction. *F > gi|15484370|gb|BI592948.1|BI592948 *F > CG1670 Obp19b. There are 10 ESTs, several of which appear to be independent *F clones, *F > which determine the coding sequence to be as below. The initiator Met is *F downstream *F > of that originally predicted but upstream of that predicted by either *F Robertson or *F > Galindo & Smith. *F > See gi|15532575|gb|BI630365.1|BI630365 or gi|3833396|gb|AI238538.1|AI238538. *F atgatgcagtg cagccgaatg acgacgacgt tgaagatgac gaaccttctg ctagcagtgg *F cctgcgccgc cgtgctgatg ggatcggcga cggcggacga ggaggagggg tccatgaccg *F tggacgaggt ggtggagctg atcgagccct ttggcgacgc ctgcacgcca aagccgtcga *F gggagaacat cgtcgagatg gtgctgaaca aggaggacgc caagcacgag accaagtgct *F tccgccactg catgctggag cagttcgagc tgatgcccga ggatcagttg cagtataacg *F aggacaagac ggtcgatatg atcaacatga tgttcccgga tcgcgaggac gacggcaggc *F gcatcgtcaa gacctgcaac gaggagctaa aggccgagca ggacaagtgc gaggcagccc *F acgggatcgc tatgtgcatg ctgcgcgaga tgcgctcttc gggcttcaag attcccgaga *F tcaaggaatg a *F > CG8462 Obp56e. There are 24 ESTs which confirm the original gene prediction. *F > gi|15531268|gb|BI629058.1|BI629058 *F > CG18111 Obp99a. I agree with Robertson and Galindo & Smith that the first *F exon was not *F > identified in the original gene prediction. I don't understand why the *F intron report of *F > Steve Mount is here as the gene was not originally predicted to have an *F intron. However, *F > the intron in his report is definitely the actual one but with incorrect *F ends. There *F > are 15 ESTs, of which several were independently obtained, which confirm the *F following *F > coding sequence. See gi|15506074|gb|BI610549.1|BI610549, *F > gi|13692677|gb|BF500840.2|BF500840 *F atgaaggtt ttcgttgcca *F tctgcgtgct gattggactg gcctccgccg actatgtggt gaagaaccga cacgacatgc *F tggcctaccg cgatgagtgc gtcaaggagc tggccgtgcc cgtggatctg gtggagaagt *F accagaagtg ggagtacccc aacgacgcca agacccagtg ctacatcaag tgcgtcttca *F ccaagtgggg cctgttcgac gtccagagcg gtttcaacgt ggagaacatc caccaacagc *F tggtgggcaa ccacgctgac cacaacgagg ccttccacgc ctccttggcc gcctgcgtgg *F acaagaacga gcagggatcc aatgcctgcg agtgggccta ccgcggagcc acctgtctgc *F tgaaggagaa cctggcccag atccagaaga gcctggcccc gaaggcctag *F > CG12665 We propose the name Obp8a. I agree completely with Robertson that *F the first *F > exon was not identified in the original gene prediction. This is another *F 4-Cys isoform. *F > There are four ESTs confirming the following coding sequence. *F > See gi|4247802|gb|AI404715.1|AI404715 (orientation backwards). *F ATGATGCGGAGATCACAGATCGGTTTGCTCAGCAGGCTGCTGCTGTTGCTGCTGGTGGTGGAACTGACGC *F CCCCTGCTATTCCGGTGCCCATGCGATCCTCACCCCAATCGCTGGCCCTACTGCGAGCACGGGATCAGTG *F CGGCAGGGAGCTGACTGCTGCCCAGCGTCTGCAGCTGGACAGGATGCAATTCGAGGATGCTGCCCATGTG *F CGTCACTATCTCCATTGCTTCTGGTCACGGCTGCAGCTCTGGCTGGATGAGACCGGATTCCAGGCACAGC *F GCATCGTTCAGAGTTTCGGCGGCGAGAGGCGTCTCAATGTGGAGCAGGCACTGCCAGCCATCAACGGGTG *F CAATGCGAAAACGAGCTCCAGAGGATCGGGCGCTCAGACAGTGGTCGACTGGTGTTTCCGTGCCTTTGTC *F TGCGTGCTGGCCACTCCAGTCGGTGAGTGGTACAAGCGCCACATGTCCGATGTCATCAATGGGAATGCCTAG *F > CG7584 We propose the name Obp99c. This is another 4-Cys isoform. The *F original gene *F > prediction is confirmed by 36 ESTs. See gi|15521987|gb|BI626462.1|BI626462. *F > CG7592 Obp99b. The original gene prediction is confirmed by 12 ESTs. *F > See gi|3868657|gb|AI261132.1|AI261132I261132 *F Dr. Laurie Graham *F Department of Biochemistry *F Queen's University *F Kingston, Ontario *F K7L 3N6 *F PH: 613-533-2984 *F FAX: 613-533-2497 *F grahamla@post.queensu.ca # *U FBrf0149615 *a Schneider *b M. *t 2002.6.17 *T personal communication to FlyBase *u FlyBase error report for CG18250 on Mon Jun 17 03:24:49 2002. *F Date: Mon, 17 Jun 2002 03:24:50 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: martina.schneider@medkem.lu.se *F Subject: FlyBase error report for CG18250 on Mon Jun 17 03:24:49 2002 *F Error report from Martina Schneider (martina.schneider@medkem.lu.se) *F Gene or accession: CG18250 *F Release: 1 *F Gene annotation error *F Gene CG18250 has incorrect exon/intron structure or translation start site. *F Comments: The in release 2 predicted exon \#8 has been removed in release 3. *F We find exon 8 is present in ld11619. # *U FBrf0149616 *a Peifer *b M. *t 2002.5.14 *T personal communication to FlyBase *u FlyBase error report for CG4211 on Mon Apr 15 12:40:31 2002. *F Date: Tue, 14 May 2002 11:57:37 \-0700 (PDT) *F From: Sima Misra *F To: Mark Peifer *F Cc: *F Subject: Re: FlyBase error report for CG4211 on Mon Apr 15 12:40:31 2002 *F Dear Mark, *F Thanks very much for your error report. We will certainly use it to *F reannotate nonA (CG4211), and make sure that the release 3 annotation maps *F the protein in SWP and GenBank. This work should happen sometime this *F summer; the X chromosome is the last one to be reannotated. *F Sima Misra *F FlyBase BDGP *F > Error report from Mark Peifer (peifer@unc.edu) Gene or accession: *F > CG4211 Release: 2 Gene annotation error Gene CG4211 has incorrect *F > exon/intron structure or translation start site. Comments: Why does *F > FlyBase list NonA as encoding a 230 aa protein, while X55902, the *F > Genbank entry, and Q0404, the SWISS PROT entry, suggest it is more *F > like 700 amino acids? *F > # *U FBrf0149617 *a Levis *b B. *t 2002.6.25 *T personal communication to FlyBase *u *F Date: Tue, 25 Jun 2002 11:08:55 \-0400 *F To: flybase-help@morgan.harvard.edu, *F 'Kathy Matthews' *F From: Robert Levis *F Subject: The P-element insertion in l(3)10418 is in the CG14858 gene *F I think that the CG14858 and l(3)10418 genes can be merged, since the *F P-element insertion in l(3)10418 is in an exon of the CG14858 gene, *F according to release 2 annotations. I am not aware of any *F experimental evidence that this P-element has been shown to be *F responsible for the lethal mutation, but I gather that such evidence *F is not required for the merging of these genes. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149618 *a Levis *b B. *t 2002.6.25 *T personal communication to FlyBase *u *F Date: Tue, 25 Jun 2002 12:43:41 \-0400 *F To: flybase-help@morgan.harvard.edu, *F 'Kathy Matthews' *F From: Robert Levis *F Subject: KG00759 is an allele of both CG18078 and CG9273 *F Cc: *F KG00759 is listed in FlyBase as an allele of CG18078. The P-element *F insertion in KG00759 is inserted in an exon of CG9273, which is *F nested in an intron of CG18078. I don't know what convention you use *F in such cases of an insertion mutation hitting two genes. However *F you annotate this, the gene report for CG9273 should note that *F KG00759 is a mutant allele of this gene; the gene report currently *F states that there are no known mutant alleles. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149619 *a Llimargas *b M. *t 2002.6.3 *T personal communication to FlyBase *u *F From kalk2 Fri May 31 15:53:42 2002 *F To: mlcbmc@cid.csic.es *F Subject: FlyBase query *F Dear Dr Llimargas, *F I am currating your paper for FlyBase: *F Llimargas and Lawrence, 2001, Proc. Natl. Acad. Sci. USA 98(25): 14487--14492 *F .. *F DWnt6 DWnt8 DWnt10 \- Please could you tell me which CG gene prediction *F each of these genes corresponds to so we can rename them in our *F database. I will curate this piece of information as a personal *F communication to FlyBase so that users can see how we knew this piece *F of information. *F Thankyou in advance for your help. *F Kerry. *F \---------------------------------------------------------------------- *F Kerry Knight, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: kalk2@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 3 Jun 2002 11:49:22 \+0200 *F To: Kerry Knight *F From: Marta Llimargas *F Subject: Re: FlyBase query *F dear Kerry, *F .. *F > DWnt6 DWnt8 DWnt10 \- Please could you tell me which CG gene prediction *F >each of these genes corresponds to so we can rename them in our *F >database. I will curate this piece of information as a personal *F >communication to FlyBase so that users can see how we knew this piece *F >of information. *F According to the Wnt homepage (http://www.stanford.edu/~rnusse/wntwindow.html) *F DWnt-6 in GadFly (CG4969) *F DWnt-8 in GadFly (CG8458) *F DWnt-10 in GadFly (CG4971) *F best wishes *F marta *F \-- *F \------------------------------------------------------------------------------ *F --------------------------------------- *F Marta *F Llimargas *F Institut de Biologia Molecular de Barcelona (IBMB) *F CSIC *F C/ Jordi Girona 18-26 *F 08034 Barcelona *F Spain *F Tel: (34) 93 400 61 35 *F Fax: (34) 93 204 59 04 *F Email: mlcbmc@cid.csic.es *F \------------------------------------------------------------------------------ *F --------------------------------------- # *U FBrf0149621 *a Laverty *b T. *t 2002.5.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 14 17:28:53 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-kuz.DN}2 insertion *F The following information accompanied stocks sent to the Bloomington Stock *F Center by Todd Laverty, UC Berkeley (4/02). *F P{UAS-kuz.DN}2 is a homozygous viable and fertile second chromosome *F insertion. The construction and transformation of P{UAS-kuz.DN} were *F described in Pan and Rubin, 1997 (Cell 90: 271-280; FBrf0096030). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149622 *a Brodsky *b M. *t 2002.5.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 14 17:40:28 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: p53 constructs and insertions *F The following information was provided to the Bloomington Stock Center by *F Michael Brodsky, UMass Medical School (5/02). *F P{GUS-p53.259H}3.1 is a homozygous viable and fertile third chromosome *F insertion. *F P{GUS-p53.Ct}3.1 is a third chromosome insertion. *F The construction and transformation of P{GUS-p53.259H} and P{GUS-p53.Ct} *F were described in Brodsky et al., 2000 (Cell 101:103-113; FBrf0127026). *F P{GUS-p53} is the full length, wild type p53 ORF cloned into the P{GUS} *F vector. P{GUS-p53}2.1 is a homozygous viable and fertile second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149623 *a Cook *b K. *t 2002.5.15 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed May 15 16:33:49 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(3L)ri-XT1 cytology *F I have examined polytene chromosome squashes of Df(3L)ri-XT1 and found the *F breakpoints 77E2-4;78A2-4. The 77E1-4 quadruplet is attenuated, but a *F distal portion is still present. The 78A5,6 doublet is present, but the *F 78A1,2 doublet is absent. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149624 *a Campbell *b K. *t 2002.5.9 *T personal communication to FlyBase *u *F From campbell@morgan.harvard.edu Thu May 09 20:45:53 2002 *F Subject: Problem with DGC and linked Swiss Prot records AE003587 *F To: cambridge-curators@morgan.harvard.edu *F DGC:SD10843 which is supposed to correspond to CG4775 l\(2\)k00619 FBgn0031312 *F is incorrect and does not match. It is being linked to Swiss Prot: SWP:Q95R38 *F and this is the incorrect protein sequence. DGC:SD10843 corresponds to the *F protein sequence on the opposite strand. *F There is a correct DGC for CG4775 (RH48586). I have made the correct annotation *F in apollo on AE003587. Let me know if I should put any notations in the text *F editor for this gene. *F Kathy # *U FBrf0149625 *a Stefancsik *b R. *t 2002.5.22 *T personal communication to FlyBase *u *F From raymund.stefancsik@cbrc2.mgh.harvard.edu Wed May 22 22:35:46 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: l(2)k05909 *F Hi, *F I have some unpublished results which may be of interest to FlyBase and *F the Drosophila research community. Our genetic analysis of l(2)k05909 *F suggests that it is an allele of synaptotagmin (syt). In the attachement *F of this e-mail I am sending a txt *F file describing our experimental results. *F Best regards, *F \-- *F Raymund Stefancsik, Ph.D. *F Cutaneous Biology Research Center *F Massachusetts General Hospital/Harvard Medical School *F Building 149, 13th Street *F Charlestown, MA 02129 *F USA *F Phone: 617-724-8290 Fax: 617-726-4453 *F E-mail: raymund.stefancsik@cbrc2.mgh.harvard.edu *F Genetic analysis of two insertions, l(2)k05909 and EP(2)1039, *F located close to Alp23B *F Our laboratory is interested in TGF-beta signal transduction, *F so we tested two P element insertions in the proximity of *F Alp23B (Activin Like Protein at 23B, FBgn0031461) as *F potential alleles of this gene. The results are summarized below. *F A) l(2)k05909 is lethal in trans to sytT77 or *F deficiencies uncovering syt (see Table 1). The only lethal *F mutation on the sytT77 chromosome is in syt, because it can *F be rescued by a synaptotagmin cDNA transgene (sytelav.PD.), *F when homozygous or in trans to sytN6 (Littleton et al., *F 1994, Calcium dependence of neurotransmitter release and rate *F of spontaneous vesicle fusions are altered in Drosophila *F synaptotagmin mutants. Proc. Natl. Acad. Sci. USA 91(23): *F 10888--10892 FBrf0077252). From the lack of *F complementation between l(2)k05909 and sytT77 we conclude *F that l(2)k05909 (FBgn0022151) is an allele of synaptotagmin *F (syt, FBgn0004242). *F B) The sequence recovered from the 3' end of P element *F EP(2)1039 (FBti0010740, GenBank \#AQ254821) maps very close to *F the upstream region of the Alp23B transcribed region. *F EP(2)1039 is homozygous viable (see Table 1) with no apparent *F abnormalities. It is not an allele of Cy (FBgn0000403), which *F also maps to this region. *F _____________________________________________________ *F TABLE 1: *F l(2)k05909 EP(2)1039 Cy1 *F Df(2L)C144 \- \+ \- *F Df(2L)JS31 \- \+ \- *F Df(2L)N6 \- \+ \+ *F Df(2)JS17 \+ \+ \+ *F sytT77 \- nd \+ *F Cy67.3 \+ \+ \- *F EP(2)1039 \+ \+ \+ *F _____________________________________________________ *F TABLE LEGEND: *F \+: viable in trans (with no visible abnormalities) *F \-: lethal in trans *F nd: not determined *F FLY STOCKS USED: *F y1 w67c23; P{w+mC=lacW}l(2)k05909k05909/CyO (SOURCE: *F Bloomington Stock Center, BL-10586) *F Df(2L)C144, dppd-ho ed1/In(2LR)Gla, wgGla-1 Bc1 *F EgfrE1 (SOURCE: Bloomington Stock Center, BL-90) *F Dp(2;1)JS13, z1 w11E4; Df(2L)JS31, dppd-ho/CyO CyO *F (SOURCE: Bloomington Stock Center, BL-1581) *F y1 w*; Df(2L)N6, P{w+mC=lacW}B8-2-30, sytN6/CyO *F (SOURCE: Bloomington Stock Center, BL-3910) *F Df(2L)JS17, dppd-ho/CyO, P{ry+t7.2=en1}wgen11 (SOURCE: *F Bloomington Stock Center, BL-1567) *F y1 w*; P{w+mC=lacW}sytT77/CyO (SOURCE: Bloomington *F Stock Center, BL-4377) *F EP(2)1039/CyO (balancer is a guess R.S.) (SOURCE: Szeged *F Stock Center) *F Cy67.3 dppd*/In(2LR)Gla, wgGla-1 (SOURCE: Bloomington *F Stock Center, BL-3918) *F \- *F Raymund Stefancsik, Ph.D. *F Cutaneous Biology Research Center *F Massachusetts General Hospital/Harvard Medical School *F Building 149, 13th Street *F Charlestown, MA 02129 *F USA *F Phone: 617-724-8290 Fax: 617-726-4453 *F E-mail: raymund.stefancsik@cbrc2.mgh.harvard.edu # *U FBrf0149626 *a Sweeney *b S. *t 2002.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu May 23 20:34:17 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-Hsap\KCNJ2.EGFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Sean Sweeney, University of California at San Francisco (4/02). *F P{UAS-Hsap\KCNJ2.EGFP}7 is a homozygous viable and fertile third chromosome *F insertion. *F P{UAS-Hsap\KCNJ2.EGFP}1 is a homozygous viable and fertile second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149627 *a Laverty *b T. *t 2002.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed May 22 15:39:23 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: ER2-7BXE-2847 and ER2-8AXE-2684 *F The following information accompanied stocks sent to the Bloomington Stock *F Center by Todd Laverty, UC Berkeley (4/02). *F Karim et al. (Genetics 143: 315-329, 1996; FBrf0087493) isolated two *F X-ray-induced enhancers of Ras85DV12.sev which were not described in the *F paper. They are ER2-7BXE-2847 and ER2-8AXE-2684. The ER2-8A locus is *F located on chromosome 2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149628 *a Carpenter *b A.T.C. *t 2002.4.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sat May 25 15:47:00 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: In(3L)A54 and cu2 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Adelaide Carpenter, Cambridge University (4/02). *F In(3L)A54 was induced by X-ray treatment of a st1 pp red1 e4 *F chromosome. Its breakpoints are 65A7-15;69B3-C11;72E3-F4 and its new *F cytological order is *F 3Lt \- 65A7 | insertion of 6 bands of unknown origin | 69B3 \- 65A15 | 72E3 \- *F 69C11 | 72F4 \-3Rt. *F The inversion complex is associated with homozygous lethality and a *F dominant gapped wing vein phenotype involving L4. *F In combination with Df(3L)E44 or Df(3L)F10, recessive phenotypes are *F seen. These include missing mid-thorax, reduced eyes and aristae, and *F held-up wings. These recessive phenotypes may reflect effects of the 69BC *F inversion breakpoint on gv and eyg. Viability is low with many uneclosed *F pupae. When dissected, these pupae are headless (except for the presence *F of palps) or have very reduced heads. They also show the thoracic defects *F seen in the viable escapers. *F The X-ray treatment also induced a new allele of cu, called cu2, *F identified by its curled wing phenotype in combination with cu1. There *F is no cytologically visible aberration in region 86 where the cu locus *F maps. cu2 can be separated from the inversion complex by meiotic *F recombination. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149629 *a Brodsky *b M. *t 2002.5 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 21 16:35:23 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{ey-FLP.B} construct and insertion *F The following information was provided to the Bloomington Stock Center by *F Michael Brodsky, UMass Medical School (5/02). *F He described the construction of P{ey-FLP.B} as follows: *F 'My ey-FLP construct was generated by cloning the 0.2kb eyeless enhancer *F fragment described by Halder and Gehring into the pHZ50pL enhancer *F detection vector (at the XbaI and KpnI sites) and then replacing the *F TATA-box and lacZ gene (cutting at KpnI, SalI, and blunting) with a *F fragment containing the minimal hsp70 promoter and FLP gene from Golic's *F pDM420 derivative (from the NruI site in hsp70 promoter and XbaI site *F adjacent to pDM420).' *F P{ey-FLP.B}3.1 is an insertion into a Sb1-marked TM3 [most likely *F TM3-rSS, K.C.]. This balancer variant will be denoted by the balancer *F short name TM3, P{ey-FLP.B}3.1, Sb1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149630 *a Adachi-Yamada *b T. *t 2002.5.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon May 27 18:21:03 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-Src42A.CA}5 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Takashi Adachi-Yamada, Kobe University (1/02). *F P{UAS-Src42A.CA}5 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149631 *a Hofmeyer *b K. *t 2002.5.30 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Mar 18 09:54:28 2002 *F To: hofmeyer@saturn.med.nyu.edu *F Subject: Helping FlyBase: ADRC-10422 *F Dear Kerstin, *F We are currently curating the abstracts for the upcoming 43rd *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'out of step, a new mutation affecting R7 projection to the optic lobe in *F Drosophila.' *F You mention a gene that is new to FlyBase, oos. Do you know yet which *F of the Genome Project CG annotations your gene corresponds to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its map location, as this is valuable *F information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From hofmeyer@saturn.med.nyu.edu Thu May 30 02:43:58 2002 *F Subject: Re: Helping FlyBase: ADRC-10422 *F To: Rachel Drysdale *F Dear Rachel *F i am very sorry to reply so late \- *F in case you still need this information *F ' out of step' is a mutation in the D.m. Liprin 1 alpha gene, encoded by *F CG11199. *F this gene has been described for the first and only time so far ( to my *F knowledge) in the following publication *F Neuron 2002 Mar 28;34(1):27-38 *F Drosophila liprin-alpha and the receptor phosphatase Dlar control synapse *F morphogenesis. *F Kaufmann N, DeProto J, Ranjan R, Wan H, Van Vactor D. *F best regards *F Kerstin # *U FBrf0149632 *a Stocker *b H. *t 2002.5.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu May 30 18:39:48 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(3L)X-21.2 *F The following information was communicated to the Bloomington Stock Center *F by Hugo Stocker, University of Zurich (5/02). *F Df(3L)X-21.2 was generated by X-ray treatment of a P{lacW} insertion in 71EF. *F Polytene chromosome squashes by Kevin Cook showed the breakpoints 71F1;72A2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149633 *a Nitabach *b M. *t 2002.5.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu May 23 21:43:48 2002 *F To: Rachel Drysdale (Genetics) *F Subject: P{UAS-dORKdelta-C} and P{UAS-dORKdelta-NC} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mike Nitabach, New York University (4/02). *F P{UAS-Ork1.&Dgr;-C}1 is a third chromosome insertion. The chromosome *F carrying this insertion is homozygous viable and male fertile, but is *F homozygous female sterile. *F P{UAS-Ork1.&Dgr;-C}2 is a homozygous viable and fertile, third chromosome *F insertion. It expresses the modified ORK1 channel more weakly than the *F P{UAS-dORKdelta-C}1 insertion. *F P{UAS-Ork1.&Dgr;-NC}1 is a homozygous viable and fertile, third chromosome *F insertion. It expresses the non-conducting, modified ORK1 channel at a *F similar level as P{UAS-dORKdelta-C}1 in pacemaker neurons. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0149634 *a Levis *b R. *t 2002.6.6 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Thu Jun 06 18:58:58 2002 *F To: flybase-help@morgan.harvard.edu, *F 'Kathy Matthews' *F Subject: P{lacW}v(2)k16105k16105 is an allele of fl(2)d? *F The P-element insertion P{lacW}v(2)k16105k16105 is inserted an what *F is annotated in release 2 as the first exon of fl(2)d. There is a *F stock of this insertion strain in the Bloomington stock center, *F whereas the FB gene report for fl(2)d reports that there are no *F mutant alleles available from stock centers. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149635 *a Levis *b R. *t 2002.6.10 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Mon Jun 10 19:11:35 2002 *F To: flybase-help@morgan.harvard.edu, *F 'Kathy Matthews' *F Subject: l(3)04053 is an allele of CG11238 *F The insertion in l(3)04053 is in exon 1 of CG11238, according to *F Release 2 annotations. The FlyBase gene report for this gene reports *F only the wild type allele and the Bloomington genotype for this *F insertion has it as an allele of the l(3)04053 gene. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149637 *a McClelland *b A. *t 2002.5.10 *T personal communication to FlyBase *u *F From acm9@duke.edu Fri May 10 18:25:37 2002 *F Subject: Insertion map position *F To: *F FlyBase, *F I am a technician in Rick Fehon's laboratory at Duke University, and in the *F course of a project I have mapped the p-element insertion P{ry+t7.2=S38M}1 *F (FBti0001649) using inverse PCR. I got this insertion from the Bloomington *F Stock Center (BL-10427). From both 5' and 3' flanking sequence this *F insertion maps to CG16980. CG16980 is listed as having a map location of *F 71E1-2 and the insertions map location should be updated accordingly. If you *F would like, I could send you the 5' and 3' flanking sequences. *F Thank you, *F Andy McClelland *F \----------------- *F Duke University *F Developmental, Cell, and Molecular Biology Group *F B333 LSRC *F Research Drive *F Durham, NC 27708 # *U FBrf0149638 *a Levis *b R. *t 2002.6.12 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Wed Jun 12 20:17:59 2002 *F To: flybase-help@morgan.harvard.edu, *F Subject: The P-element in l(2)k05713 is inserted in CG8256 *F The P-element insertion in the mutant l(2)k05713 is inserted in an *F intron of all annotated (Release 2) transcripts of CG8256. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149639 *a Levis *b R. *t 2002.6.17 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Mon Jun 17 22:48:45 2002 *F To: flybase-help@morgan.harvard.edu, *F 'Kathy Matthews' *F Subject: The P-element insertion in l(3)02640 is in the CG9165 gene *F The P-element insertion in l(3)02640 is in an intron of the CG9165 *F gene. This isn't evident from the gene report of CG9165 or the Stock *F Center genotype. *F ...Bob *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149640 *a Levis *b R. *t 2002.6.24 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Mon Jun 24 18:05:33 2002 *F To: flybase-help@morgan.harvard.edu, *F 'Kathy Matthews' *F Subject: P-element in l(3)03022 is inserted in eIF-5C *F According to release 2 annotations, the P-element in l(3)03022 is *F inserted in the transcribed sequences of the eIF-5C gene. There is a *F stock of this insertion mutant at Bloomington. The FlyBase report *F for this gene does not list l(3)03022 as an allele and states that *F there are no mutant alleles at the stock center. I am not aware of *F any experimental evidence that this P-element has been shown to be *F responsible for the lethal mutation. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0149641 *a Nairz *b K. *t 2002.6.28 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Fri Jun 28 15:58:28 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: Df(3R)96B (stock 4531) *F comments: The distal breakpoint of Df(3R)96B has been wrongly mapped: *F \-Analysis of polytene chromosome squashes indicates a chromosomal *F deletion between 96A21 and 96B11. *F \-Using a local high-density SNP map the borders have been placed between *F tok and tld on the proximal side and between CG6031 and CG13646 on the distal *F side. As CG6238/slingshot (96B10/B11) is complemented by the deficiency, the *F distal breakpoint must be situated somewhere between CG6031 (at 96B8) and Osbp *F (96B10). *F Original data will be published in PNAS soon. *F Sincerely, *F Knud *F mailto: flybase-help@morgan.harvard.edu *F realname: Knud Nairz *F reply-to: nairz@zool.unizh.ch *F Sent from computer wildmaus.unizh.ch # *U FBrf0149675 *a Courey *b A. *t 2002.7.19 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Fri Jul 19 20:23:10 2002 *F Envelope-to: R.Foulger@gen.cam.ac.uk *F Delivery-date: Fri, 19 Jul 2002 20:23:10 +0100 *F Date: Fri, 19 Jul 2002 14:23:38 -0500 (EST) *F From: fbmailer@bio.indiana.edu *F Reply-to: courey@chem.ucla.edu *F To: flybase-help@morgan.harvard.edu *F Subject: CG6854 *F Content-Length: 826 *F *F comments: The flybase entry for CG6854 is somewhat misleading. You *F indicate that the gene encodes a CTP synthase. In fact the locus *F appears to encode at least two different proteins that result from *F alternative splicing. One is a CTP synthase and the other is a *F transcription factor with homology to Adf-1, Stonewall, and Dip3. The *F protein sequence to which you have included a link, Q9VUK9, is the *F transcription factor sequence. The transcription factor coding region *F may be completely contained within an intron of the CTP synthase *F transcript. But there is also a third transcript , which, if real, *F gives rise to a hybrid protein that is part transcription factor and *F part CTP synthase. *F mailto: flybase-help@morgan.harvard.edu *F realname: Al Courey *F reply-to: courey@chem.ucla.edu *F Sent from computer d45-39.chem.ucla.edu # *U FBrf0149778 *a Weihofen *b A. *t 2002.7.28 *T personal communication to FlyBase *u *F From andreas.weihofen@bc.biol.ethz.ch Sun Jul 28 00:26:12 2002 *F Envelope-to: R.Foulger@gen.cam.ac.uk *F Delivery-date: Sun, 28 Jul 2002 00:26:12 +0100 *F User-Agent: Microsoft-Entourage/9.0.2509 *F Date: Sun, 28 Jul 2002 01:27:01 +0200 *F Subject: Shanti;update suggestion *F From: Andreas Weihofen *F To: *F Mime-version: 1.0 *F Content-Type: multipart/alternative; boundary='B_3110664421_2473966' *F Content-Length: 1652 *F *F *F The gene product of shanti (FBgn0031260 ) was recently identified as *F orthologue of the human intramembrane cleaving presenilin-type aspartic *F protease signal peptide peptidase *F *F Weihofen A, Binns K, Lemberg MK, Ashman K, Martoglio B. *F Identification of signal peptide peptidase, a presenilin-type aspartic *F protease. *F Science. 2002 Jun 21;296(5576):2215-8. *F *F Kind regards *F *F Andreas Weihofen *F -- *F Andreas Weihofen *F Institute of Biochemistry *F ETH Hoenggerberg *F 8093 Zurich *F Switzerland # *U FBrf0150764 *a Laszlo *b T. *t 2002 *T personal communication to FlyBase *u *F From laszlo@titus.u-strasbg.fr Fri Jul 05 07:57:19 2002 *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (cy1628) *F Dear Chihiro Yamada, *F Thank you for your mail. *F The real dmTAF12 should be CG17358. *F However, according to our new nomenclature CG15632 should be called *F dmTAF12L (L for 'like') in the future. *F Best regards, *F Laszlo *F >Dear Dr Tora, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Tora, 2002, Genes Dev. 16(6): 673--675 *F > *F >I have a quick question I was hoping you could answer for me. *F > *F >You rename a gene TAFII30(alpha); to TAF12. We have two *F >genes in our records this could be: *F > *F >Taf30(alpha) *F >CG17358 *F >TBP-associated factor 30kD subunit (alpha) *F > *F > *F >Taf30(alpha)-2 *F >CG15632 *F >transcription factor TFIID, 30kD (alpha)-subunit 2 *F > *F >Could you tell me which of these two genes is TAF12? *F > *F >Thanks *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >United Kingdom. FAX: 01223-333992 *F > \- Memes don't exist. Tell your friends. (Anon) \- *F >---------------------------------------------------------------------- *F INSTITUT DE GENETIQUE ET DE BIOLOGIE MOLECULAIRE ET CELLULAIRE (IGBMC) *F INSERM \- U.184, CNRS \- LGME, ULP, *F Parc d'Innovation, *F 1, rue Laurent Fries, *F BP 10142, *F 67404 ILLKIRCH Cedex, *F C.U. de STRASBOURG, *F FRANCE *F Fax: from France 03 88 65 32 01 from abroad 33 3 88 65 32 01 *F Tel.: from France 03 88 65 34 44 (or 41) from abroad 33 3 88 65 34 44 (or 41) *F Web site: http://biblio-igbmc.u-strasbg.fr/Tora # *U FBrf0150770 *a Skaer *b H. *t 2002 *T personal communication to FlyBase *u *F Date: Wed, 7 Aug 2002 11:23:06 \+0100 *F To: Chihiro Yamada *F From: Helen Skaer *F Subject: Re: FDlyBase Query (cy1667) *F Dear Chihiro *F Thank you for your message about the CtBGal4 \- here is something about *F it......... *F The CtB-Gal4 line refers to a promoter fusion construct that we have *F generated. The construct contains a 3.3kb promoter fragment from the cut *F gene (Jack & DeLotto, 1995) fused to the GAL4 coding sequence and inserted *F into pCaSpER. In this line, Gal4 is expressed in the embryonic Malpighian *F tubules throughout development and is maintained into larval life. This *F line also drives low levels of expression in the foregut. *F Jack & DeLotto (1995) Genetics 139: 1689-1700 *F Please let me know if you need any further information. *F best wishes helen *F Helen Skaer *F Department of Zoology *F University of Cambridge *F Downing Street *F Cambridge CB2 3EJ *F tel 01223 763189 (office) *F 01223 336640 ( lab) *F fax 01223 336676 *F \---------------------------------------------------------------------- *F To: hs17@cam.ac.uk *F Subject: FDlyBase Query (cy1667) *F From: Chihiro Yamada *F Date: Wed, 31 Jul 2002 12:03:21 \+0100 *F Dear Helen, *F I am currently curating your paper for FlyBase: *F Sudarsan et al., 2002, Development 129(4): 935--944 *F I have a quick question I was hoping you could answer for me, *F In your materials and methods you mention a previously unpublished Gal4 *F line that you call CtB-Gal4. *F Can you give me some information about this line? *F Is is an enhancer trap or a promoter fusion? If it is the former can *F you tell me which transposon was used to generate this line (e.g. *F P{GawB}). If the latter can you give me some molecular details about *F tyhe transposon? Does the CtB stand for the gene C-terminal Binding *F Protein? *F Any new information you give me will be curated as a personal *F communication from you to FlyBase, if thats fine with you. *F Thanks *F Chihiro *F \---------------------------------------------------------------------- *F Chihiro Yamada. *F FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: c.yamada@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F United Kingdom. FAX: 01223-333992 *F Memes don't exist. Spread the Word. *F \---------------------------------------------------------------------- # *U FBrf0150776 *a Robertson *b H. *t 2002.7.5 *T personal communication to FlyBase *u *F Date: Fri, 05 Jul 2002 16:25:58 \-0500 *F From: Hugh Robertson *F Subject: More on Or19a duplication *F To: Celniker_Susan , *F Misra_Sima , *F Millburn_Gillian , *F Carlson_John , *F Warr_Coral , *F Vosshall_Leslie , *F Moriyama_Etsuko , *F Kim_Junhyong *F Dear Susan et al., *F I've spent the afternoon fighting with the apparent duplication of Or19a, *F about 50kbp downstream and in opposite orientation, that several of us are *F aware of. From the BACR11H15 sequence in GenBank I discovered a few new neat *F things that I think convincingly bring the DmOr number to 62. *F First, the duplication is much longer, extending ±850bp upstream and ±700 *F downstream for a total of around 2940bp and an average divergence of just 1%. *F The entire Or19a sequence is below, coding exons in upper case. *F Or19a \- *F MDISKVDSTRALVNHWRIFRIMGIHPPGKRTFWGRHYTAYSMVWNVTFHICIWVSFSVNLLQSNSLETFCESLCVTMP *F HT *F LYMLKLINVRRMRGQMISSHWLLRLLDKRLGCDDERQIIMAGIERAEFIFRTIFRGLACTVVLGIIYISASSEPTLMY *F PT *F WIPWNWRDSTSAYLATAMLHTTALMANATLVLNLSSYPGTYLILVSVHTKALALRVSKLGYGAPLPAVRMQAILVGYI *F HD *F HQIILRLFKSLERSLSMTCFLQFFSTACAQCTICYFLLFGNVGIMRFMNMLFLLVILTTETLLLCYTAELPCKEGESL *F LT *F AVYSCNWLSQSVNFRRLLLLMLARCQIPMILVSGVIVPISMKTFTVMIKGAYTMLTLLNEIRKTSLE *F aatagatattgaagaatagaatgagaaaaattgtttcaaattattcaaaagtaggtgtaaggaatggcgtaaccaatg *F tg *F cttttggtatggcggtagaaatttacaaaataaacaataaaacgaagaaaaatctacacatctttcaaaagtgtgagc *F gt *F ggcagtagtgggcggatggtgggcgtgccgaaaagttgtttggcatatcgataaaatctatagaactaacaaaaatgt *F aa *F aagaacatcttctatagttcctgagatcgagacgttcatacgaaatgaccgacggatggattgaaatcgccagctcga *F ca *F cggctattgatccggatcaagaatgtatatactttatatcgagtatacccttgtactctacgaggaacggatatataa *F aa *F atgtttcacttcctttgtcgcgtctcgcagccgccaaaattttgaagacacagttcaactatgcgccaccgcagctat *F gc *F agccttatcttcgttaactaagctatctcccatcgcagcgatttgccaaacaactatttgccacgcccacaaatcggc *F ca *F aacctttcacgccgttgaacacaacgctttagataaaataacgctttaaaaaaaaaatttaaacaaaagtgtgggaat *F aa *F ttataaaaaccttttcatttaccagaaagctaagtggcgctaatgatgataaggaggcgattgatttgcgaaaaatga *F tt *F gtcattgtaattgctggtggtggttcaaaatcgaactttttcggtgtaatttgcagcaatttgcataatttgcggcat *F ag *F gtggcttattggatgaatataaaaaggtcggaacgcggagagctgcttcaaagaggcgaacaaccATGGACATATCGA *F AG *F GTGGATTCAACGAGGGCTCTGGTTAACCACTGGCGCATCTTCAGGATTATGGGAATCCATCCGCCGGGCAAGAGGACC *F TT *F CTGGGGTCGCCACTACACGGCGTACTCCATGGTGTGGAACGTAACCTTCCACATCTGCATCTGGGTGTCCTTTTCGGT *F CA *F ATCTCCTGCAGTCCAATTCGCTGGAGACTTTCTGCGAGAGCCTCTGCGTGACCATGCCGCACACGCTCTACATGCTTA *F AG *F CTGATCAATGTCCGTCGGATGCGCGGCCAGATGATCAGCAGCCACTGGTTGCTCCGTCTCTTGGACAAGCGGCTTGGC *F TG *F CGACGACGAACGCCAGATCATTATGGCCGGCATCGAGCGGGCCGAGTTCATATTCCGCACCATTTTTCGCGGCCTTGC *F GT *F GCACCGTCGTCCTTGGCATCATCTACATATCCGCGTCCAGCGAGCCCACGCTGATGTACCCCACCTGGATTCCCTGGA *F AC *F TGGAGGGACAGCACCTCCGCCTACCTGGCCACCGCCATGCTGCACACGACCGCCCTCATGGCGAATGCGACACTTGTC *F CT *F CAATCTGAGCTCCTATCCGGGCACCTACCTCATCCTGGTCAGTGTCCACACCAAGGCGCTCGCCCTGCGGGTCTCCAA *F AT *F TGGGATATGGCGCGCCACTACCGGCGGTTCGGATGCAGGCCATTCTGGTTGGTTACATCCACGACCACCAGATCATTT *F TG *F CGgtgagtgtcaggaaatctcatctcccaatgcaagaacttttaaagcatttcgggagttttgaccttcatcgaaagg *F cg *F tatgtacacacactttggcgtgccaaacatcttcattgtcattgaagattattatatcctttttctcaactacagCCT *F CT *F TCAAGTCACTGGAGAGATCCCTTTCGATGACCTGCTTTCTGCAGTTCTTCAGCACGGCGTGTGCGCAGTGCACAATCT *F GC *F TACTTTCTACTCTTCGGGAACGTCGGGATCATGAGGTTCATGAATATGTTGTTCCTGCTGGTGATCCTCACCACGGAG *F AC *F CCTCCTTCTCTGCTACACGGCGGAGCTACCTTGCAAGGAAGGGGAGAGCCTCCTGACCGCTGTCTACAGCTGCAACTG *F GC *F TGTCCCAGTCGGTTAACTTTCGGAGACTCCTGCTCCTGATGCTCGCACGCTGCCAGATTCCAATGATCCTGGTCTCCG *F GC *F GTAATTGTGCCCATCAGCATGAAGACCTTCACGGTGgtgagtgctgtacaaagctaacatttacccaccttatcagcg *F at *F tttttgcagATGATTAAGGGAGCGTACACCATGCTTACTCTGCTGAATGAAATTCGTAAAACGTCCCTTGAATAGaac *F tg *F aaatcgtaggcaatatagtatatataattcatatgttcaaacacacacttgggaaacagtcgctttcctattcgcttc *F gc *F actgactttatctgagtaagaggtatccgatagtcgcagaactcggcaatggcattctctttggtctacccgcagtcg *F cc *F agaatttcctaaatttgttattttcaaacatgacaactttgaagacgcagttcagcatagacgcatcgatgaagaata *F aa *F taatcgttcgctaaagtaaataaacattaatcgtttgaaatcactacataaaaatcaatcgccttttcattgctcgaa *F aa *F aagcaaataagcattttaattatttttttttattattatttgggaataagttaacagtaatcgcatatactagatgtc *F ct *F gcaaatgttgattttggcttagtactcctcggattacatgcatgagctgaaataggtacagtatttcacattccttct *F cc *F ttttgggcaatcagcctctccatgtcctgtatttcctcatcgatgcgctggatctcttcaaaaagaaaaagcttcaat *F tg *F tcgaagaagaagcctgtaaggactgcttaccctccgtgtaagtaggcgaacttatcatattcgatacactcggctgct *F ca *F ggatccttcctcgtcaaatactcgttgtttctagttgaatatgcattctcgttcgactt *F Second, within the coding region there are now six single bp changes, three of *F which change the encoded amino acids, so this needs to be annotated as a *F separate slightly divergent gene, presumably Or19b by the accepted gene *F nomenclature for Ors. .. *F Or19b \- *F MDISKVDSTRALVNHWRIFRIMGIHPPGKRTFWGRHYTAYSMVWNVTFHICIWVSFSVNLLQSNSLETFCESLCVTMP *F HT *F LYMLKLINVRRMRGEMISSHWLLRLLDKRLGCADERQIIMAGIERAEFIFRTIFRGLACTVVLGIIYISASSEPTLMY *F PT *F WIPWNWKDSTSAYLATAMLHTTALMANATLVLNLSSYPGTYLILVSVHTKALALRVSKLGYGAPLPAVRMQAILVGYI *F HD *F HQIILRLFKSLERSLSMTCFLQFFSTACAQCTICYFLLFGNVGIMRFMNMLFLLVILTTETLLLCYTAELPCKEGESL *F LT *F AVYSCNWLSQSVNFRRLLLLMLARCQIPMILVSGVIVPISMKTFTVMIKGAYTMLTLLNEIRKTSLE *F aatagatattggggaatagaatgagaaaaattgtttcaaattattcaaaagtaggtgtaaggaatggcgtaaccaatg *F tg *F tttttggtatggcggtagaaatttacaaaataaacaataaaacgaagaaaaatctacacatctttcaaaagtgtgagc *F gt *F ggcagtagtgggcggatggtgggcgtgccgaaaagttgtttggcaaatcgataaaatctatagaactaacaaaaatgt *F aa *F aagaacatcttctatagttcctgagatcgagacgttcatacgaaatgaccgacggatggaccaaatcgccagctcgac *F ac *F ggctattgatccggatcaagaatgtatatactttatatcgagtatacccttttactcttcgagtaacgagtataaaaa *F tg *F tttcacttcctttgtcgcgtctcgcagccgccagaattttgaagacacagttcaactatgcgccaccgcagctatgca *F gc *F cttatcttcgttaactaagctatctcccatcgcagcgatttgccaaacaactatttgccacgcccacaaatcggccaa *F ac *F ctttcacgccgttgaacacaacgctttagataaaataacgctttaaaaaaaaaatttaaacaaaagtgtgggaataat *F ta *F taaaaaccttttcatttaccagaaagctaagtggcgctaatgatgataaggaggagattgatttgcgaaaaatgattg *F tc *F attgtaattgctggtggtggttcaaaatcgaactttttcggtgtaatttgcagcaatttgcataatttgcggcatagg *F tg *F gcttattggatgaatataaaaaggtcggaacgcggagagctgcttcaaagaggcgaacaaccATGGACATATCGAAGG *F TG *F GATTCAACGAGGGCTCTGGTTAACCACTGGCGCATCTTCAGGATTATGGGAATCCATCCGCCGGGCAAGAGGACCTTC *F TG *F GGGTCGCCACTACACGGCGTACTCCATGGTGTGGAACGTAACCTTCCACATCTGCATCTGGGTGTCCTTTTCGGTCAA *F TC *F TCCTGCAGTCCAATTCGCTGGAGACTTTCTGCGAGAGCCTCTGCGTGACCATGCCGCACACGCTCTACATGCTTAAGC *F TG *F ATCAATGTCCGTCGGATGCGCGGCGAGATGATCAGCAGCCACTGGTTGCTCCGTCTCTTGGACAAGCGGCTTGGCTGC *F GC *F CGACGAACGCCAGATCATTATGGCCGGCATCGAGCGGGCCGAGTTCATATTCCGCACCATTTTTCGCGGCCTTGCGTG *F CA *F CCGTCGTCCTTGGCATCATCTACATATCCGCGTCCAGCGAGCCCACGCTGATGTACCCCACCTGGATTCCCTGGAACT *F GG *F AAGGACAGCACCTCCGCCTACCTGGCCACCGCCATGCTGCACACGACCGCCCTCATGGCGAATGCGACACTTGTCCTC *F AA *F TCTGAGCTCCTATCCGGGCACCTACCTCATCCTGGTCAGTGTCCACACCAAGGCGCTCGCCCTGCGGGTCTCCAAATT *F GG *F GATATGGCGCGCCACTACCGGCGGTTCGGATGCAGGCCATTCTGGTTGGGTACATCCACGACCACCAGATCATTTTGC *F Gg *F taagtgtcaggaaatctcatctcccaatgcaagaacttttaaagcatttcgggagttttgaccttcatcgaaaggcgt *F at *F gtacacacactttggcgtgccaaacatcttcattgtcattgaagattattatatcctttttctcaactacagCCTCTT *F CA *F AGTCACTGGAGAGATCCCTTTCGATGACCTGCTTTCTGCAGTTCTTCAGCACGGCGTGTGCGCAGTGCACAATCTGCT *F AC *F TTTCTACTCTTCGGGAACGTCGGGATCATGAGGTTCATGAATATGTTGTTCCTGCTGGTGATCCTCACCACGGAGACC *F CT *F TCTTCTCTGCTACACGGCGGAGCTACCTTGCAAGGAAGGGGAGAGCCTCCTGACCGCTGTCTACAGCTGCAACTGGCT *F GT *F CCCAGTCGGTAAACTTTCGGAGACTCCTGCTCCTGATGCTCGCACGCTGCCAGATTCCAATGATCCTGGTCTCCGGCG *F TA *F ATTGTGCCCATCAGCATGAAGACCTTCACGGTGgtgagtgctgtacaaagctaacatttacccaccttatcagcgatt *F tt *F ttgcagATGATTAAGGGAGCGTACACCATGCTTACTCTGCTGAATGAAATTCGTAAAACGTCCCTTGAATAGaactga *F aa *F tcgtaggcaatatagtatatataattcatatgttcaaacacacacttgggaaacagtcgctttcctattcgcttcgca *F ct *F gactttatctgagtaagaggtatccgatagtcgcagaactcggcaatggcattctctttggtctacccgcagtcgcca *F ga *F atttcctaaatttgttattttcaaacatgacaactttgaagacgcagttcagcatagacgcatcgatgaagaataaat *F aa *F tcgttcgctaaagtaaataaacattaatcgtttgaaatcactacataaaaatcaatcgccttttcattgctcgaaaaa *F ag *F caaataagcattttaattatttttttttattattatttggcaataagttaacagtaatcgcatatactagatgtcctg *F ca *F aatattgattttggcttagtactcctcggattacatgcatgagctgaaataggtacagtatttcacattccttctcct *F tt *F tggacaatcagcctctccatgtcctgtatttcctcatcgatgcgctggatctcttcaaaaagaaaaagcttcaattgt *F cg *F aagaagaagcctgtaaggactgcttaccctccgtgtaagtaggcgaacttatcatattcgatacactcggctgctcag *F ga *F tccttcctcgtcaaatactcgttgtttctagttgaatatgcattcgcgttcgactt *F Hugh *F Hugh M. Robertson, Professor *F Department of Entomology, University of Illinois at Urbana-Champaign *F 320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801 *F Phone 217-333-0489; FAX 217-244-3499; email hughrobe@uiuc.edu *F WWW http://www.life.uiuc.edu/robertson/lab.html # *U FBrf0150777 *a Robertson *b H. *t 2002.7.11 *T personal communication to FlyBase *u *F Date: Thu, 11 Jul 2002 00:48:17 \-0500 *F From: Hugh Robertson *F Subject: Update on Gr47b annotation *F To: Millburn_Gillian , Amrein_Hubert , *F Scott_Kristin , Carlson_John *F Dear colleagues, *F I've been working on the Drosophila Or/Gr molecular evolution again and as *F part of that have a minor change to propose in Gr47b, which I think needs to *F be 18aa longer, providing a more decently alignable C-terminus. My proposed *F sequence is below. *F Hugh *F MQRDDGFVYCYGNLYSLLLYWGLVTIRVRSPDRGGAFSNRWTVCYALFTRSFMVICFMATVMTKLRDPEMSAAMFGHL *F SPLVKAIFTWECLSCSVTYIEYCLSLDLQKDRHLKLVARMQEFDRSVLMVFPHVQWNYRRARLKYWYGTVIVGFCFFS *F FSISLIFDTTRCTCGIPSTLLMAFTYTLLTSSVGLLGFVHIGIMDFIRVRLRLVQQLLHQLYQADDSSEVHERIAYLF *F EMSKRCSFLLAELNGVFGFAAAAGIFYDFTIMTCFVYVICQKLLEREPWDPEYVYMLLHVAIHTYKVVITSTYGYLLL *F REVGDLISKRNCMHLLSQYSRYFSGQDVARRKTEDFQHWRMHNRQAAMVGSTTLLSVSTIYLVYNGMANYVIILVQLL *F FQQQQIKDHQLTSGKDVDIVGPMGPITHMD *F Hugh M. Robertson, Professor *F Department of Entomology, University of Illinois at Urbana-Champaign *F 320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801 *F Phone 217-333-0489; FAX 217-244-3499; email hughrobe@uiuc.edu *F WWW http://www.life.uiuc.edu/robertson/lab.html # *U FBrf0150780 *a Basler *b K. *t 2002.7.9 *T personal communication to FlyBase *u *F To: basler@molbio.unizh.ch *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Tue, 9 Jul 2002 10:41:22 \+0100 *F Dear Dr. Basler, *F I am curating your paper for FlyBase: *F Gerlitz and Basler, 2002, Genes Dev. 16(9): 1055--1059 *F and I have a few questions about some of the constructs and mutants you *F used. *F 1. wf141 ( = superscript). *F You state that this mutant was described in: *F Melendez et al., 1995, Genetics 141(4): 1507--1520 *F We have a record of a number of lethal mutations from that paper, but *F none of the alleles have a symbol like '141'. Do you know which lethal *F complementation group from that paper wf141 corresponds to (the *F complementation groups are l(3)72Ab, l(3)72Ac, l(3)72Ad, l(3)72CDa, *F l(3)72CDb, l(3)72CDc, l(3)72CDd, l(3)72CDe and l(3)72CDf) \- then I *F could merge the lethal complementation group in with the wf gene in *F FlyBase. *F 2. .. scalloped-Gal4 *F is this the 'SG29.1' line mentioned in : *F Milan et al., 1997, Proc. Natl. Acad. Sci. USA 94(11): 5691--5696 *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F Date: Fri, 12 Jul 2002 22:04:32 \+0200 *F To: Gillian Millburn (Genetics) *F From: Konrad Basler *F Subject: Re: FlyBase query *F Dear Gillian, *F here the answers to your questions: *F > *F > *F >1. wf141 ( = superscript). *F > *F >You state that this mutant was described in: *F > *F >Melendez et al., 1995, Genetics 141(4): 1507--1520 *F > *F >We have a record of a number of lethal mutations from that paper, but *F >none of the alleles have a symbol like '141'. Do you know which lethal *F >complementation group from that paper wf141 corresponds to (the *F >complementation groups are l(3)72Ab, l(3)72Ac, l(3)72Ad, l(3)72CDa, *F >l(3)72CDb, l(3)72CDc, l(3)72CDd, l(3)72CDe and l(3)72CDf) \- then I *F >could merge the lethal complementation group in with the wf gene in *F >FlyBase. *F wf141 is l(3)72CDf). We called it wf141 because of the protein *F truncation at amino acid position 141. *F >2. .. scalloped-Gal4 *F > *F >is this the 'SG29.1' line mentioned in : *F > *F >Milan et al., 1997, Proc. Natl. Acad. Sci. USA 94(11): 5691--5696 *F No, this is S278, a Gal4 insertion line from our collection (see *F Gerlitz, O., Nellen, D., Ottiger,M., and Basler K. 2002. A screen for *F genes expressed in Drosophila imaginal discs. Int. J. Dev. Biol. 46: *F 173-176). *F Please do not hesitate to contact me if there are further questions. *F Thanks for your help! *F Kind regards, *F Konrad # *U FBrf0150781 *a Tycowski *b K. *t 2002.7.12 *T personal communication to FlyBase *u *F To: steitz@biomed.med.yale.edu *F Subject: FlyBase query: snoRNAs *F Cc: gm119@gen.cam.ac.uk, mhuang@morgan.harvard.edu *F From: Gillian Millburn (Genetics) *F Date: Wed, 10 Jul 2002 16:33:00 \+0100 *F Dear Dr. Steitz, *F I have just curated your paper for FlyBase: *F Tycowski and Steitz, 2001, Europ. J. Cell Biol. 80(2): 119--125 *F which discusses the genes 'dUHG1' and 'dUHG2' and the snoRNA genes in *F their introns. *F It would be really useful to FlyBase if we knew the sequences of dUHG1, *F dUHG2 and the snoRNAs so that they could be annotated correctly onto *F the genome. Do you have these sequences (preferably in FASTA format) ? *F If you send us the sequences, we would record your e-mail as a personal *F communication to FlyBase so that users could see where we got the *F information from, and we would use the sequences to annotate the genes *F correctly, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F Date: Fri, 12 Jul 2002 12:17:44 \-0400 *F From: Kazio Tycowski *F Subject: Drosophila UHG genes *F To: gm119@gen.cam.ac.uk *F Dear Dr. Millburn, *F In response to your email to Dr. Steitz, I am sending you sequences of *F Drosophila UHG1 and UHG2 cDNAs as well as snoRNAs encoded within the UHG *F introns. Note that some of the snoRNA genes are found in several introns *F (Fig. 2 in our paper); the variants of the same snoRNA differ by only *F few nucleotides. I am sending you only one representative sequence of *F each snoRNA. *F The 5' ends of the cDNAs have been experimentally mapped and the 3' *F ends terminate at the first AAUAAA polyadenylation signal; the authentic *F 3' ends are not known. *F Please let me know if you have any further questions. *F Sincerely, *F Kazimierz Tycowski *F Research Scientist in Joan A. Steitz Lab *F \------------------------------------------------------------------------------ *F -- *F >U14snoRNA.Seq *F ttttactgatgattaacttcaacaccttttgcggtttccaccagaaagcttcggcttaatgatggtctaaggcgtctga *F ct *F >U15snoRNA.Seq *F ctcaaatgatgaaacagatgacgagactgaaggaacaaattctcgacttggcgctgtatttttcagtgcctcgttatat *F atttgttcatttaagatgattcggcgatctgatg *F >U27snoRNA.Seq *F gttctgtgatgtcaaaccaatagacaagcatataaccgaacaatcatgttgattttcacacgactgagc *F >U25snoRNA.Seq *F cttctctgatgatcttttacacagacctgtactgagctcataaccgtgcagaaaattgtaaactgaaa *F >U29snoRNA.Seq *F actcaatgatgaaacatttacccagctcgctttgatattcaatgaagagaactgttccatatccaacgcaaaaataaac *F tgctgact *F >U31snoRNA.Seq *F cttacatgatgattttattcgtaccgccccagtctgaagagacgtgttgattggttatttatactgata *F >U76snoRNA.Seq *F tgtaaatgatgatttattattatttgctactcttgaaggtcattgatgaatactttcaccttaaaacctgatg *F >Snr38snoRNA.Seq *F gggcaatgatgaacactttatttaagccaaacagttatccctgtcataagaataattgatgataaaagttgctgatt *F >Dros-UHG1-cDNA.Seq *F cacttctttttttcgttcaaaatggacggtcgcgttttctgcttgaattctcaactaaaatttaacaaatgggatgttg *F tgcggtagcaggaaatggaactgaaaataactatggaaaaaacagtttttatggctttctgtcaaatttatccacacgt *F gtctcaaagtaagatggtctattcgaataaaagagatttcgcttacggcaaagatagacggaagattatatgattcgaa *F ataagttcttataaattcctagggatctgtaatgtcggactcctattttccttaattccggaaaaatcaaaatgtttag *F aatttaagtcagtgatcctggccgtctgcttaaggaaaaatgagaaaatgagaaaatggttgacacaaatcataatcat *F aatcaaacaaaatatacaatccacggataaatcttttctcagaacaatggaacacagaaatgcggtcaagtttggaaat *F atgagaaatattttttcaagtttactcgttgttttcactgggttttgcgctttgtagttaaacgggaacgcaggaaagc *F ttagatattgaacgaataaaatttaaaaatggtatttgcacactatataaatcaagctgcgaaggaattttggtgatta *F atgaagattcgctaatatcaacatattacaaaataaaacccgattgaggatggctaaaaatctaaaggtattccccgtg *F ttgactgacagccgatttgcaatggctttttagtacaaggtcttcataatatgcagtacccaagttggtttggattata *F atatgtatttccaagatgtttgaagattaccagggaaaaagtctatctaccctgcaagattattaaggcttgaactaaa *F gagcggtcgttttgcatggaagatcccaaatgaacgcagaatattaataaggtaatacctgacatctggctttgtgatt *F cagctgaacttttgtggtatcgtgcgaattgcaaagatcgaccaacgccatttcactccaaaccccaaattgctgtagt *F catttaaacaaataaa *F >Dros-UHG2-cDNA.Seq *F cattttatttcaagactcgaaatgtgaacaatattgaaaccctaagcatttggataaatcgaaatccgggcactccacg *F tgcatggtgcatgtgagacacctcgggacaacatgtgagcccgttgagacaccttgggcactgagcccactggagacgc *F cattgtcacattggagaatttacattctggtttgaggttatgtatgggtccagctgcagcagcgagcacatggatttac *F cgataaagatcaactcagaaatgtggtttatcgcaggcaaatataactaaatgagctcgtttattcgactgtataagag *F tgacgttgccttgtgtaaaagatcttgatataaaaagaattggacatcttcaaatactaaaataaa *F \------------------------------------------------------------------------------ *F -- # *U FBrf0150782 *a Zimniak *b P. *c H. *d Benes *t 2002.7.15 *T personal communication to FlyBase *u *F From: 'Zimniak, Piotr' *F To: 'Gillian Millburn' *F Cc: 'Benes, Helen' *F Subject: RE: Insect GSTs *F Date: Sun, 14 Jul 2002 19:29:52 \-0500 *F Glutathione S-transferases: nomenclature *F The glutathione S-transferase (GST) family includes multiple members *F in many species. Thus, the early nomenclature was extremely confusing, with *F each lab using different naming schemes. Often several names were assigned *F to a single protein. In 1992, the major players in the field agreed on a *F unifying and systematic nomenclature, initially for human GSTs (1). The *F basic principles of that nomenclature are as follows: *F (1) Each gene that encodes a GST subunit (cytosolic GSTs are dimeric) has a *F unique name. *F (2) The subunits are grouped into classes, designated by capital letters. *F (Classes have been previously identified on the basis of substrate *F specificity, immunological crossreactivity, sequence similarity, and the *F ability to form dimers with members of the same class, but not other *F classes. Examples of classes are A (also known as Alpha), P (known as Pi), *F etc.). *F (3) New GST subunits are given systematic names when their full sequence *F becomes known. A name consists of the class letter followed by an Arabic *F numeral. The numbering is consecutive (in the order of sequence publication) *F and has no meaning beyond that. *F (4) The name of the protein reflects the dimeric structure of GSTs. For *F example, a homodimer of the Alpha subunit that was first to be sequenced is *F GSTA1-1, and a heterodimer of two different Alpha subunits could be GSTA1-2 *F (the name 'GSTA1-A2' is redundant since dimers are formed only within, but *F not between classes). The genes encoding the subunits are called GSTA1 and *F GSTA2, respectively. *F (5) Allelic variants of the same locus receive the same Arabic numeral, *F followed by a lowercase letter. *F The above nomenclature, which resembles similar schemes established *F for other multigene families such as cytochromes P450, ABC transporters, and *F others, has been quickly extended to other species, mostly mammals, by using *F a prefix denoting the species. Thus, hGSTA1-1 is human, while mGSTA1-1 is *F murine etc. *F The nomenclature of insect GSTs remains chaotic for historical *F reasons and for lack of a generally accepted system. To rectify this, *F several of the leading GST researchers suggested an extension of the *F mammalian naming convention to invertebrates (2). According to this *F proposal, the species designator is typically derived from the systematic *F name of the organism, e.g., Dm for Drosophila melanogaster or Ag for *F Anopheles gambiae. The major problem is with classes. Some insect GSTs can *F be assigned to existing classes, for others, new classes need to be *F established. To maintain a unified and conflict-free nomenclature, we *F strongly suggest consulting with Dr. Philip Board prior to naming a class or *F a single GST. *F For Drosophila melanogaster, the systematic nomenclature (2) would *F abolish the traditional designations of Class I and Class II. GSTs of Class *F I become class Delta; e.g., an enzyme originally described by C.-P. Tu's *F group (3) would be named DmGSTD1-1. The previous Class II is heterogeneous. *F The Drosophila GST previously named GST-2 (4) belongs to the Sigma class, *F and is therefore named DmGSTS1-1. To the recently described GST-3 (5), in *F consultation with Dr. Board we assigned the name DmGSTE1-1 (class Epsilon). *F Other Drosophila GSTs cloned by Board's group are listed in ref. (2). A *F correspondence table of the old and new names is given in reference (2). A *F slightly modified and extended version of this table is also shown below. *F Although names seemingly don't matter, it is obviously important to *F keep them consistent and unequivocal. Since a systematic and generally *F accepted nomenclature for vertebrate GSTs already exists, we strongly urge *F the adoption of its extension to insect GSTs, as proposed in (2). *F References: *F 1. Mannervik, B., Awasthi, Y.C., Board, P.G., Hayes, J.D., Di Ilio, C., *F Ketterer, B., Listowsky, I., Morgenstern, R., Muramatsu, M., Pearson, W.R., *F Pickett, C.B., Sato, K., Widersten, M. and Wolf, C.R. (1992). Nomenclature *F for human glutathione transferases. Biochem. J. 282: 305-308. *F 2. Chelvanayagam, G., Parker, M.W. and Board, P.G. (2001). Fly fishing for *F GSTs: a unified nomenclature for mammalian and insect glutathione *F transferases. Chem. Biol. Interact. 133: 256-260. *F 3. Toung, Y.-P.S., Hsieh, T. and Tu, C.-P.D. (1993). The glutathione *F S-transferase D genes. A divergently organized, intronless gene family in *F Drosophila melanogaster. J. Biol. Chem. 268: 9737-9746. *F 4. Beall, C., Fyrberg, C., Song, S. and Fyrberg, E. (1992). Isolation of a *F Drosophila gene encoding glutathione S-transferase. Biochem. Genetics 30: *F 515-527. *F 5. Singh, M., Silva, E., Schulze, S., Sinclair, D.A.R., Fitzpatrick, K.A. *F and Honda, B.M. (2000). Cloning and characterization of a new theta-class *F glutathione-S-transferase (GST) gene, gst-3, from Drosophila melanogaster. *F Gene 247: 167-173. *F \--------------------------------------------------------------------------- *F |Old name | Proposed | Class | Flybase ID | Other | Comments | *F | | systematic | | | identifiers | | *F | | gene name | | | | | *F \--------------------------------------------------------------------------- *F | GST-1 | DmGSTD1 | Delta | FBgn0001149 | | | *F | DmGST-1 | | | | | | *F \--------------------------------------------------------------------------- *F | DmGST21 | DmGSTD2 | Delta | FBgn0010038 | CG4181 | | *F \--------------------------------------------------------------------------- *F | DmGST22 | DmGSTD3 | Delta | | CG4381 | footnote a | *F \--------------------------------------------------------------------------- *F | DmGST23 | DmGSTD4 | Delta | FBgn0010040 | CG11512 | | *F \--------------------------------------------------------------------------- *F | DmGST24 | DmGSTD5 | Delta | FBgn0010041 | CG12242 | | *F \--------------------------------------------------------------------------- *F | DmGST25 | DmGSTD6 | Delta | FBgn0010042 | CG4423 | | *F \--------------------------------------------------------------------------- *F | DmGST26 | DmGSTD7 | Delta | FBgn0010043 | CG4371 | | *F \--------------------------------------------------------------------------- *F | DmGST27 | DmGSTD8 | Delta | FBgn0010044 | CG4421 | | *F \--------------------------------------------------------------------------- *F | none | DmGSTD9 | Delta | | | | *F \--------------------------------------------------------------------------- *F | none | DmGSTD10 | Delta | | | | *F \--------------------------------------------------------------------------- *F | GST2 | DmGSTS1 | Sigma | FBgn0010226 | | | *F \--------------------------------------------------------------------------- *F | GST3 | DmGSTE1 | Epsilon | FBgn0034335 | CG5164 | footnote b | *F \--------------------------------------------------------------------------- *F | none | DmGSTT1 | Theta | | | footnote c | *F \--------------------------------------------------------------------------- *F | none | DmGSTZ1 | Zeta | | | footnote c | *F \--------------------------------------------------------------------------- *F | none | DmGSTO1 | Omega | | | footnote c | *F \--------------------------------------------------------------------------- *F a DmGSTD3 is listed in Flybase as a pseudogene. However, an EST is listed in *F Flybase (clone LP11313) that perfectly matches DmGSTD3, indicating that the *F gene is transcribed. We have evidence (manuscript in preparation) that the *F open reading frame of DmGSTD3 can be translated and that the resulting *F protein is enzymatically active. Thus, DmGSTD3 is probably a functional *F gene. *F b DmGSTE1 is a member of a gene cluster containing at least ten genes (named *F DmGSTE1 to DmGSTE10) (manuscript in preparation). *F c Reference (2). No further information (such as sequence) is currently *F published. *F \------------------------------------------------------------------------------ *F >DmGSTD1 *F MVDFYYLPGSSPCRSVIMTAKAVGVELNKKLLNLQAGEHLKPEFLKINPQ *F HTIPTLVDNGFALWESRAIQVYLVEKYGKTDSLYPKCPKKRAVINQRLYF *F DMGTLYQSFANYYYPQVFAKAPADPEAFKKIEAAFEFLNTFLEGQDYAAG *F DSLTVADIALVATVSTFEVAKFEISKYANVNRWYENAKKVTPGWEENWAG *F CLEFKKYFE *F >DmGSTD2 *F MDFYYMPGGGGCRTVIMVAKALGLELNKKLLNTMEGEQLKPEFVKLNPQH *F TIPTLVDNGFSIWESRAIAVYLVEKYGKDDYLLPNDPKKRAVINQRLYFD *F MGTLYESFAKYYYPLFRTGKPGSDEDLKRIETAFGFLDTFLEGQEYVAGD *F QLTVADIAILSTVSTFEVSEFDFSKYSNVSRWYDNAKKVTPGWDENWEGL *F MAMKALFDARKLAAK *F >DmGSTD3 *F MVGKALGLEFNKKIINTLKGEQMNPDFIKINPQHSIPTLVDNGFTIWESR *F AILVYLVEKYGKDDALYPKDIQKQAVINQRLYFDMALMYPTLANYYYKAF *F TTGQFGSEEDYKKVQETFDFLNTFLEGQDYVAGDQYTVADIAILANVSNF *F DVVGFDISKYPNVARWYDHVKKITPGWEENWAGALDVKKRIEEKQNAAK *F >DmGSTD4 *F MDFYYSPRSSGSRTIIMVAKALGLELNKKQLRITEGEHLKPEFLKLNPQH *F TIPTLVDNGFAIWESRAIAVYLVEKYGKDDSLFPNDPQKRALINQRLYFD *F MGTLHDSFMKYYYPFIRTGQLGNAENYKKVEAAFEFLDIFLVGQDYVAGS *F QLTVADIAILSSVSTFEVVEFDISKYPNVARWYANAKKITPGWDENWKGL *F LQMKTMYEAQKASLK *F >DmGSTD5 *F MDFYYSPRGSGCRTVIMVAKALGVKLNMKLLNTLEKDQLKPEFVKLNPQH *F TIPTLVDNGFSIWESRAIAVYLVEKYGKDDTLFPKDPKKQALVNQRLYFD *F MGTLYDSFAKYYYPLFHTGKPGSDEDFKKIESSFEYLNIFLEGQNYVAGD *F HLTVADIAILSTVSTFEIFDFDLNKYPNVARWYANAKKVTPGWEENWKGA *F VELKGVFDARQAAAKQ *F >DmGSTD6 *F MDLYNMSGSPSTRAVMMTAKAVGVEFNSIQVNTFVGEQLEPWFVKINPQH *F TIPTLVDNLFVIWETRAIVVYLVEQYGKDDSLYPKDPQKQALINQRLYFD *F MGTLYDGIAKYFFPLLRTGKPGTQENLEKLNAAFDLLNNFLDGQDYVAGN *F QLSVADIVILATVSTTEMVDFDLKKFPNVDRWYKNAQKVTPGWDENLARI *F QSAKKFLAENLIEKL *F >DmGSTD7 *F MTNIFIQTLLRLIVLWLFFHKYEHSDSKKSVYFFAFRSSHVSVTMPNLDL *F YNFPMAPASRAIQMVAKALGLELNSKLINTMEGDQLKPEFVRINPQHTIP *F TLVDNGFVIWESRAIAVYLVEKYGKPDSPLYPNDPQKRALINQRLYFDMG *F TLYDALTKYFFLIFRTGKFGDQEALDKVNSAFGFLNTFLEGQDFVAGSQL *F TVADIVILATVSTVE *F >DmGSTD8 *F MDFYYHPCSAPCRSVIMTAKALGVDLNMKLLKVMDGEQLKPEFVKLNPQH *F CIPTLVDDGFSIWESRAILIYLVEKYGADDSLYPSDPQKKAVVNQRLYFD *F MGTLFQSFVEAIYPQIRNNHPADPEAMQKVDSAFGHLDTFLEDQEYVAGD *F CLTIADIALLASVSTFEVVDFDIAQYPNVASWYENAKEVTPGWEENWDGV *F QLIKKLVQERNE *F >DmGSTD9 *F MLDFYYMLYSAPCRSILMTARALGLELNKKQVDLDAGEHLKPEFVKINPQ *F HTIPTLVDDGFAIWESRAILIYLAEKYDKDGSLYPKDPQQRAVINQRLFF *F DLSTLYQSYVYYYYPQLFEDVKKPADPDNLKKIDDAFAMFNTLLKGQQYA *F ALNKLTLADFALLATVSTFEISEYDFGKYPEVVRWYDNAKKVIPGWEENW *F EGCEYYKKLYLGAILNKQ *F >DmGSTD10 *F MDLYYRPGSAPCRSVLMTAKALGVEFDKKTIINTRAREQFTPEYLKINPQ *F HTIPTLHDHGFALWESRAIMVYLVEKYGKDDKLFPKDVQKQALINQRLYF *F DMGTLYKSFSEYYYPQIFLKKPANEENYKKIEVAFEFLNTFLEGQTYSAG *F GDYSLADIAFLATVSTFDVAGFDFKRYANVARWYENAKKLTPGWEENWAG *F CQEFRKYF *F >DmGSTS1 *F MADEAQAPPAEGAPPAEGEAPPPAEGAEGAVEGGEAAPPAEPAEPIKHSY *F TLFYFNVKALAEPLRYLFAYGNQEYEDVRVTRDEWPALKPTMPMGQMPVL *F EVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDFRLKIAV *F VSYEPEDEIKEKKLVTLNAEVIPFYLEKLEQTVKDNDGHLALGKLTWADV *F YFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEPIKAWIEKRPVTEV *F >DmGSTE1 *F MSSSGIVLYGTDLSPCVRTVKLTLKVLNLDYEYKEVNLQAGEHLSEEYVK *F KNPQHTVPMLDDNGTFIWDSHAIAAYLVDKYAKSDELYPKDLAKRAIVNQ *F RLFFDASVIYASIANVSRPFWINGVTEVPQEKLDAVHQGLKLLETFLGNS *F PYLAGDSLTLADLSTGPTVSAVPAAVDIDPATYPKVTAWLDRLNKLPYYK *F EINEAPAQSYVAFLRSKWTKLGDK *F \------------------------------------------------------------------------------ # *U FBrf0150784 *a Viragh *b E. *c T. *d Szlanka *t 2002.7.17 *T personal communication to FlyBase *u *F To: kissi@nucleus.szbk.u-szeged.hu *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Tue, 16 Jul 2002 11:41:57 \+0100 *F Dear Dr. Kiss, *F I am curating a paper for FlyBase: *F Mason et al., 2002, Genetics 161(1): 157--170 *F in which they report a personal communication from you which indicates *F that phenotypes previously attributed to the importin-alpha2 gene *F Pendulin are in fact due to a second site mutation. *F On page 161 they state: *F Two early studies reported that flies homozygous for a null allele of *F the importin 2 gene (oho-31) die during development while exhibiting an *F overgrowth of hematopoietic tissues (KUSSEL and FRASCH 1995 ; TOROK et *F al. 1995 ). However, these phenotypes were subsequently found to be due *F to a second site mutation (I. KISS, personal communication). *F The mutant line they are talking about is 'l(2)k14401'. *F ( = superscript) *F As a consequence of this, I am in the process of splitting out the *F 'oho31' phenotype of the 'l(2)k14401' line into a separate gene from *F Pendulin in FlyBase. There will end up being two separate genes \- *F Pendulin and oho31. The l(2)k14401 line will end up with 2 alleles *F associated with it \- 'Penk14401a' (associated with the insertion in *F the 5' end of Pendulin) and 'oho31k14401b'. *F I am trying to partition the phenotypic information we currently have *F under 'l(2)k14401' into the correct allele records. *F >From what they say in the Mason Genetics paper, the lethality and *F overgrown hematopoietic tissue phenotypes should end up under *F 'oho31k14401b' and not under 'Penk14401a'. *F 1. In your paper: *F Torok et al., 1995, J. Cell Biol. 129(6): 1473--1489 *F there are a number of other phenotypes mentioned for the 'l(2)k14401' *F line. They are: *F \- overgrowth of gonads. *F \- testes filled with small single cells, lacking spermatogonial cysts. *F \- most imaginal discs reduced in size, and abnormal in shape and *F folding pattern. *F \- genital discs larger than normal *F \- brain hemispheres smaller than normal, ventral ganglion often longer *F than normal. *F \- ring gland reduced in size. *F For each of these phenotypes, I am not sure whether they are due to an *F effect on Pen or oho31 i.e. do they belong under Penk14401a or *F oho31k14401b \- do you know which phenotypes belong under which allele? *F 2. in the paper: *F Kussel and Frasch, 1995, J. Cell Biol. 129(6): 1491--1507 *F they say that the 'l(2)k14401' line shows abnormal cell proliferation *F in the central nervous system. Do you know whether this phenotype is *F due to an effect on Pen or oho31 ? *F 3. in your paper: *F Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112 *F you mention a second line 'l(2)k14410' which is allelic to 'l(2)k14401'. *F At the moment I have put the following comment in about this line: *F FlyBase curator comment: it is not clear how many mutations or *F P-element insertions are present in the 'l(2)k14410' line. The *F lethality of 'l(2)k14410' is probably due to an effect on 'oho31' as *F 'l(2)k14410' fails to complement 'l(2)k14401' (FBrf0100624) (the *F lethality of the l(2)k14401' line is due to an effect on 'oho31'). *F However, it is not known whether or not there is also a mutation in *F 'Pen' in the 'l(2)k14410' line; since 'l(2)k14401' and 'l(2)k14410' are *F members of the same cluster (FBrf0100624), it is possible that *F 'l(2)k14410' contains a P-element insertion in 'Pen' (similar to the *F situation in 'l(2)k14401'). At present 2 alleles associated with the *F 'l(2)k14410' line have been made to accommodate the uncertainty and the *F phenotypes of this line \- 'Penk14410a' and 'oho31k14410b'. It is *F not known whether either (or both) of these (potential) alleles are *F associated with a P-element. *F (FBrf0100624 = Roch et al., 1998, Molec. gen. Genet. 257(2): 103--112) *F Do you have any information about this line which might help clear up *F whether or not this line has a mutation in Pen or oho31 or both, and *F whether or not any alleles are associated with a P-element ? *F Any information you give me about these lines would be curated as a *F personal communication to FlyBase, so that users can see how we knew *F the information, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From Kissi@nucleus.szbk.u-szeged.hu Wed Jul 17 10:08:04 2002 *F Delivery-date: Wed, 17 Jul 2002 10:08:04 \+0100 *F To: Gillian Millburn (Genetics) *F Date: Wed, 17 Jul 2002 11:05:51 \+0200 *F Subject: Re: FlyBase query *F Dear Gillian, *F Thank you for your letter and the curation of this complicated package *F of information. I can say the following: *F 1./ In l(2)k14401 homozygotes, in addition to the overgrowth of *F haematopoietic organs, all the other phenotypes (larval gonad *F overgrowth, etc., Torok et al., 1995) belong to the background mutation *F responsible for the lethality, and none of them is caused by the P *F insertion in the importin-alpha2 gene. *F 2./ 'Abnormal cell proliferation in CNS' (Kussel and Frasch, 1995) is *F the same as mentioned also in Torok et al., 1995, and also belong to *F the oho-31 background mutation. *F In fact, we removed this lethality from the l(2)k14401 chromosome by *F genetic recombination, and the remaining P insertion had no *F homozygous phenotype at all. We also generated (Torok et al., 1995) *F intragenic deletions, null alleles, in the importin-alpha2 (Pen) gene by *F remobilizing the P insertion, and the homozygotes are again viable and *F normal, except for the complete female and almost complete male *F sterility. *F 3./ As regards l(2)k14410, it does not complement l(2)k14401 lethality *F hence sharing the same oho-31 background lethal mutation. However, *F it has one P insertion in 54B and none in 31A, in the importin-alpha2 *F gene. As 14410 and 14401 probably belong to the same cluster, it is *F still possible that 14410 had the P insertion in 31A earlier but lost it *F later. Whether the importin-alpha2 gene has any mutation left behind *F by the hypothetical P loss or the new insertion at 54B has any effect, *F was not tested. Please, quote this piece of information as 'Erika Viragh and *F Tamas Szlanka, personal communication'. *F I hope this may help you. Please, write again in any case of *F uncertainty. *F With regards, *F Istvan # *U FBrf0150850 *a Bloomington Drosophila Stock Center *b ?. *t 2002.7.9 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Wed Jul 10 01:28:15 2002 *F Subject: location of P{GawB}AB1 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: location of P{GawB}AB1 *F We have mapped the insertion P{GawB}AB1 to chromosome 3. # *U FBrf0150851 *a Sweeney *b S. *t 2002.7.9 *T personal communication to FlyBase *u *F From sweeney@itsa.ucsf.edu Tue Jul 09 23:54:14 2002 *F Subject: Re: Helping FlyBase: CSH-240 *F To: Rachel Drysdale (Genetics) *F Dear Rachel *F Re: our March correspondence. We've finally submitted our 'diphthong' *F paper and we're awaiting referees reports. Since Grae has spoken *F publicly about it I reckon I can tell you that diphthong is allelic to *F spinster, originally cloned and sequenced by the Yamamoto lab. *F All the best *F Sean # *U FBrf0150852 *a Hales *b K. *t 2002.7.8 *T personal communication to FlyBase *u *F From kahales@davidson.edu Mon Jul 08 19:34:28 2002 *F Subject: RE: Helping FlyBase: ADRC-10453 *F To: 'Rachel Drysdale (Genetics)' *F Rachel, *F The gene nmd appears to correspond to CG5395. Inverse PCR with the original *F nmd allele indicated that the insertion is in the 5' UTR of CG5395, and the *F positions of the next closest genes make it unlikely that they are affected. *F Sequence analysis of the gene makes it a good candidate as well- the yeast *F homolog is an outer mitochondrial membrane protein. We are doing a rescue *F experiment to confirm that we have cloned the right gene. *F .. *F Thanks, *F Karen *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Karen G. Hales, Ph.D. *F Assistant Professor of Biology, Davidson College *F PO Box 7118, Davidson NC 28035-7118 *F (704) 894-2324 FAX (704) 894-2512 *F http://www.bio.davidson.edu/people/kahales/index.html *F From kahales@davidson.edu Tue Jul 09 19:59:50 2002 *F Subject: RE: Helping FlyBase: ADRC-10453 *F To: 'Rachel Drysdale (Genetics)' *F Rachel, *F On a separate note, it looks like CG3210 is the same as Drp1 and *F dynamin-2. I've only done some database searches and sequence gazing, *F but all the entries are referring to a dynamin homolog in 23A. *F Thanks, *F Karen *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Karen G. Hales, Ph.D. *F Assistant Professor of Biology, Davidson College *F PO Box 7118, Davidson NC 28035-7118 *F (704) 894-2324 FAX (704) 894-2512 *F http://www.bio.davidson.edu/people/kahales/index.html # *U FBrf0150854 *a Lehner *b C. *t 2002.7 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Jul 14 20:48:25 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Lehner insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Christian Lehner, University of Bayreuth (7/02). *F The following are homozygous viable and fertile, second chromosome insertions: *F P{UAS-CycB.W}II.1 *F P{UAS-Cdk2-myc}II.1 *F P{UAS-CycB3.W}II.1 *F P{UAS-CycA.W}II.2 *F P{UAS-Cdk4-myc}II.1 *F The following is a homozygous viable and fertile, third chromosome insertion: *F P{UAS-Cdk2-myc}III.1 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0150855 *a Deal *b J. *c K. *d Cook *t 2002.7.14 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Jul 14 02:38:08 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC16 *F Isolation and characterization of Df(2L)BSC16 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Df(2L)BSC16 was isolated as a P transposase-induced male recombination *F event involving P{lacW}exk12913 and P{PZ}ds05142. The deletion was *F isolated as a net1-cn1 recombinant from the cross net1 b1 cn1 *F sp1 females x net1 P{lacW}exk12913/P{PZ}ds05142 cn1; TMS, *F delta2-3 males. Polytene chromosome squashes showed the breakpoints *F 21C3-4;21C6-8. Df(2L)BSC16 failed to complement ush2, Gsc05341 and *F Df(2L)ast2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0150856 *a Bloomington Drosophila Stock Center *b ?. *t 1996.2.22 *T personal communication to FlyBase *u *F > From matthewk@fly.bio.indiana.edu Thu Feb 22 17:32:32 1996 *F > To: ag24@gen.cam.ac.uk *F > Subject: Ubx allele *F > *F > Hi Aubrey, *F > *F > One of the unmatched allele in our stocks was UbxEdK8. I didn't have *F > you create this because Thom made it, no one else ever got the stock, and *F > we decided to get rid of it \- uncharacterized allele etc. However, since *F > announcing that we were discarding it, several labs have requested it, so *F > I think you'd better create the allele afterall. *F > *F > allele: UbxEdK8 *F > discoverer: Thom Kaufman *F > mutagen: EMS *F > *F > *F > Thanks, Kathy # *U FBrf0150857 *a Ahmed *b Y. *t 2002.7.16 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 16 00:21:36 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Ahmed lethals *F The following information accompanied stocks donated by Yashi Ahmed, *F Princeton University (7/02). Eighteen lethal complementation groups were *F defined in Ahmed et al., 1998 ('Regulation of armadillo by a Drosophila APC *F inhibits neuronal apoptosis during retinal development'. Cell 93: 1171-1182 *F FBrf0102882) by screening EMS-treated chromosomes over Df(3R)3450. These *F included string and Apc. Representative alleles of the other 16 *F complementation groups are listed below with the number of alleles in the *F complementation group and the lethal phase of the allele. *F l(3)3450-AB1 8 alleles embryonic lethal *F l(3)3450-BA1 5 alleles embryonic lethal *F l(3)3450-CC6 5 alleles pupal lethal *F l(3)3450-DC2 9 alleles larval lethal *F l(3)3450-EF3 7 alleles pupal lethal *F l(3)3450-FG3 6 alleles embryonic lethal *F l(3)3450-GH2 3 alleles larval lethal *F l(3)3450-HO2 4 alleles larval lethal *F l(3)3450-IM2 4 alleles *F l(3)3450-JL1 3 alleles *F l(3)3450-KK5 1 allele larval lethal *F l(3)3450-LD1 1 allele larval lethal *F l(3)3450-MH1 1 allele larval lethal *F l(3)3450-NI5 1 allele larval lethal *F l(3)3450-OQ9 1 allele embryonic lethal *F l(3)3450-PO5 1 allele pupal lethal *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0150858 *a Deal-Herr *b M. *c K. *d Cook *t 2002.7.15 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 16 00:55:52 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2R)BSC19 *F Isolation and Characterization of Df(2R)BSC19 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center *F Df(2R)BSC19 was isolated as a P transposase-induced male recombination *F event involving P{EP}EP951 and P{lacW}l(2)s4831s4831. The deletion was *F isolated as a cn1-bw1 recombinant chromosome from the cross cn1 bw1 *F females x cn1 P{EP}EP951/P{lacW}l(2)s4831s4831 bw1 sp1; TMS, *F P{Delta2-3}/+ males. Polytene chromosome squashes showed the breakpoints *F 56F12-14;57A4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0150859 *a Das *b J. *t 2002.7.3 *T personal communication to FlyBase *u *F From jdas@princeton.edu Wed Jul 03 19:37:47 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: P{lacW}CoVa S011046 *F I have been working on the nuclear-encoded subunits of cytochrome c *F oxidase, one of which is encoded by the gene CoVa. In the FlyBase *F Report, the l(3)S011046 insertion is listed as an allele of CoVa, but *F there was some ambiguity about its location in the reference *F correspondence with Corrado Caggese. *F Using PCR, I have independently confirmed Caggese's result that *F l(3)S011046 is indeed located in the CG6946 location. Based on the *F genomic sequence of that region, the flanking sequence submitted by *F Peter Deak overlaps with CG6946 on one end, overlaps with CoVa on the *F other end, and spans the 317 bp in between. The original insertion site *F was reported to be at the CoVa end of the flanking sequence, but *F Caggese's and my results show that the P-element is actually located at *F the 24th nucleotide of the CG6946 gene. *F It would be helpful if you could remove the l(3)S011046 listing as an *F allele of CoVa, as it may be misleading to others like me who are *F looking for alleles of this gene. *F Please let me know if you have any questions. *F Thanks, *F Jayatri *F \-- *F Jayatri Das *F Ecology & Evolutionary Biology *F M156 Guyot Hall *F Princeton University *F Princeton, NJ 08544-1003 USA *F jdas@princeton.edu *F phone: 609-258-5587 *F fax: 609-258-1712 *F From rd120@gen.cam.ac.uk Thu Jul 11 13:43:37 2002 *F To: flybase-help@morgan.harvard.edu, jdas@princeton.edu *F Subject: Re: P{lacW}CoVa S011046 *F Dear Jayatri, *F Thank you for writing in about the S011046 insertion. I have looked at *F the Release 3 annotation and discover (as suggested by the gbrowse view *F of these two e.g. at *F http://www.fruitfly.org/cgi-bin/annot/gbrowse?name=gene:CG14724) *F that CoVa and CG6946 overlap at the 5' end by more than 24bp, in fact *F by 61bp. Thus the S011046 insertion seems to fall within both the *F CoVa and the CG6946 transcription units. Of course it is possible that *F the beginning of CG6946 (i.e. base pair 1) is not the same for you and *F for the Release 3 annotation. If you could send me the first few 10s *F of bps according to your mapping I can double check. *F FlyBase curation defines as alleles those cases where an insertion maps *F within a transcription unit. Thus S011046 is an allele of both CoVa *F and CG6946. The insertion (FBti object) has to be named after one of *F them and in this case it is *F FBti0009814 == P{lacW}CoVaS011046 *F though it is linked to both alleles, CG6946S011046 and CoVaS011046. *F Hope this helps explain the FlyBase data for S011046. *F Best regards, *F Rachel. *F for FlyBase curators. *F From jdas@Princeton.EDU Wed Jul 17 22:44:30 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: P{lacW}CoVa S011046 *F Dear Rachel, *F Thanks for your reply regarding the S011046 allele. According to my *F experiments, the sequence 5' to the putative insertion site is *F GACTTATTTTTCAGAGAGATTAATCAAATA; the sequence 3' to the putative insertion *F site is GCACGCGTCAAGTTGAAGATGCAGTGTGAC (the 'flanking sequence'). I have not *F confirmed this by direct sequencing, this is merely derived from a PCR *F experiment to determine which end of the 'flanking sequence' was the *F insertion site. *F I was not aware of the RA transcript of the CoVa gene that apparently *F overlaps with CG6946, as I think I was working off of a previous release. *F However, I am still confused about where the 5' end of the CoVa transcription *F unit is \-- although the annotation indicates an overlap, none of the three *F Genbank sequences for CoVa (NG_000573, NM_057869, Y09065) overlap with *F CG6946. Can you help clarify this discrepancy? Otherwise I will do a 5' *F RACE experiment to determine experimentally the sequence of the 5' end of *F CoVa and whether the P-element actually disrupts the transcription unit. *F Thanks very much, *F Jayatri *F \-- *F Jayatri Das *F Ecology & Evolutionary Biology *F M156 Guyot Hall *F Princeton University *F Princeton, NJ 08544-1003 USA *F jdas@princeton.edu *F phone: 609-258-5587 *F fax: 609-258-1712 *F From rd120@gen.cam.ac.uk Thu Jul 18 13:05:03 2002 *F To: jdas@Princeton.EDU *F Subject: Re: P{lacW}CoVa S011046 *F Dear Jayatri, *F thanks for sending me the element flanking sequence. I have delved a *F little (no, a lot!) and this is what I have found. *F I used Gadfly to get the sequence of the longest CoVa and CG6946 transcripts. *F I then used the BDGP BLAST against 'Celera/BDGP whole genome shotgun *F sequence (Release 2) (NT)' for each sequence in turn. This produces *F hits which give the coordinates for each with respect to segment *F AE003963. *F The longest CoVa transcript extends from 68276 (5' end) to 69225 (3' end) on *F AE003963. *F The longest CG6946 transcript extends from 68337 (5' end) to 64117 (3' end). *F The P-element flanking sequence you sent me extends from 68188 to 68247. *F Thus CoVa and CG6946 overlap by 61 bp, as we previously thought, but *F the 5' of CG6946 must be different than where you thought it was when *F you said 'my results show that the P-element is actually located at the *F 24th nucleotide of the CG6946 gene', since the insertion site is *F actually 120 bp within the CG6946 gene, well clear of the CoVa *F transcription unit. Having delved so deeply I have found that the 24bp *F refers to LD29934, whose 5' end is at coordinate 68242 of AE003963, *F well 3' of the current annotated 5' end of CG6946 at 68337. *F I was gratified to find that the flanking sequences for the S011046 *F insertion sent in by you and Peter agree. In conclusion the insertion *F actually maps within the CG6946 transcription unit and 58 or 59 base *F pairs 5' of the CoVa transcription unit. *F It was the Deak personal communication that allocated S011046 to CoVa, *F on the basis of proximity to CoVa but he didn't know at the time that *F the insertion site mapped within CG6946 (CG6946 was not annotated *F at that time). *F I will disentangle S011046 from the gene record for CoVa, thus all the *F phenotypic data will be ascribed to effects on CG6946. Since some of *F the effects may be due to the proximity of the insertion to CoVa this *F may not actually represent the biological reality, but until we know *F more (such as have results of rescue experiments and the like) we can *F associate S011046 with CG6946 alone. *F Hope this helps, *F Rachel. # *U FBrf0150860 *a Younger *b S. *t 2002.7.26 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Fri Jul 26 16:44:03 2002 *F Subject: construct identity for sca109-68 insertion *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Susan Younger, University of *F California San Francisco *F To: Bloomington Drosophila Stock Center *F Subject: construct identity for sca109-68 insertion *F Dated: 22 March 2002 *F Information communicated: *F The insertion associated with sca109-68 is an insertion of *F P{GawB}. # *U FBrf0150861 *a Cook *b K. *t 2002.7.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jul 29 16:23:28 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Merging fs(1)K10 allele entries *F Hi Rachel-- *F The allele entries fs(1)K102 (FBgn0000810) and fs(1)K1013-534 *F (FBal0034640) should be merged. Likewise, fs(1)K103 (FBal0004299) and *F fs(1)K1013-1121 (FBal0034639) should be merged. *F The alleles were isolated as strains 13-534 and 13-1121 in Mohler's female *F sterile screen and were given the 2 and 3 allele designations *F later. The correspondences come from some of Mohler's lab notes that I have. *F Thanks! *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0150862 *a Grosshans *b J. *c K. *d Cook *e E. *f Wieschaus *t 2002.7.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 30 18:57:05 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Deletions of P{lacW}B204 *F Deletions of P{lacW}B204 *F Jörg Grosshans, Kevin Cook and Eric Wieschaus *F The following aberrations were isolated by Jörg Grosshans in the laboratory *F of Eric Wieschaus, Princeton University, following X-ray treatment of *F P{lacW}B204. Polytene chromosome squashes were made by Kevin Cook, Indiana *F University. *F Df(3L)XG1 71C3-D1;71F2-5 *F Df(3L)XG3 70E3-4;71C2-D4 *F Df(3L)XG5 71C2-3;72B1-C1 *F Df(3L)XG6 70D3-4;71E2-5 *F Df(3L)XG7 71C1;71C2-4 *F Df(3L)XG8 71C3-D1;71F2-5 *F Df(3L)XG10 71C3-E5;71C3-E5 *F Df(3L)XG12 70D3-4;71E2-5 *F Df(3L)XG14 71A3-B1;71C2-4 *F Df(3L)XG15 71A3;71F4 *F Df(3L)XG16 71A3-B1;71C2-4 *F Df(3L)XG4, with breakpoints 71C1-2;71D2-E1;70D5-E1, is a deficiency (first *F two listed breaks) plus inversion with new cytological order 3Lt \- 70D5 | *F 71C1,2 \- 70E1 | 71E1 \- 3Rt. The 71C1,2 breakpoint is within the doublet band. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0150864 *a Ebacher *b D. *t 2002.7.31 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Thu Aug 01 15:52:29 2002 *F Subject: P{neoFRT}18A *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Dominic Ebacher, Panganiban Lab, University of *F Wisconsin, Madison *F To: Bloomington Drosophila Stock Center *F Subject: P{neoFRT}18A *F Dated: 31 July 2002 *F Information communicated: *F 'While conducting crosses recently, I encountered anomalous results with flies *F of genotype FRT(18A); eyFLP. Subsequent test crosses revealed that in the *F presence of continuous FLPase, FRT(18A) is >75% lethal and 100% of the escapers *F have severe eye and head defects. Test crosses with other FRT(18A) chromosomes *F and other FLPase stocks indicate that these phenotypes are due to the FRT(18A) *F chromosome. I now have tested eyFLPase stocks with insertions on either the *F second or third chromosome. All yield the same results with FRT(18A). None are *F lethal with FRT(19A) stocks. The lethality and developmental abnormalities are *F observed with both male and female flies carrying an FRT(18A) chromosome, so *F the defects are not due to inter-chromosomal recombination. I therefore suspect *F that the original FRT(18A) chromosome has an aberration (perhaps another FRT *F site near the one at 18A) and that this has been transmitted to recombinant *F chromosomes carrying FRT(18A). I further suspect that recombination between the *F two FRT sites deletes essential genes, leading to cell lethality and head *F defects. Consistent with my results, Seth Blair has been able to disrupt wing *F disc development using flies carrying FRT(18A) and ap-GAL4, a continuous source *F of FLPase in the wing. Thus FRT(18A) probably is cell lethal in the presence *F of continuous FLPase. In light of this, we recommend that people use the *F FRT(18A) chromosome only with transient (e.g. heat shock-inducible) FLPase, *F and that they use FRT(19A) whenever feasible.' # *U FBrf0151615 *a Lehmann *b M. *t 2002.7.1 *T personal communication to FlyBase *u *F From mlehmann@howard.genetics.utah.edu Wed Jun 12 21:17:11 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 12 Jun 2002 21:17:11 +0100 *F Date: Wed, 12 Jun 2002 16:12:46 -0700 *F From: Michael Lehmann *F X-Mailer: Mozilla 4.7C-CCK-MCD {C-UDP; EBM-APPLE} (Macintosh; I; PPC) *F X-Accept-Language: en *F MIME-Version: 1.0 *F To: flybase-updates@morgan.harvard.edu *F Subject: Pipsqueak family *F Content-Length: 2584818 *F *F Dear Flybase curator, *F *F I would like to draw your attention to a few recent publications that *F you may want to consult in updating the entries for several Drosophila *F genes. Proteins encoded by the pipsqueak (psq) gene contain an unusual, *F experimentally identified DNA-binding domain, which consists of 4 *F repeats of a 50-amino acid motif (Lehmann et al., 1998: J. Biol. Chem. *F 273, 28504--28509). Subsequent database searches confirmed that this *F motif defines a novel family of eukaryotic helix-turn-helix (HTH) *F DNA-binding proteins (Siegmund and Lehmann, 2002: Dev. Genes Evol. 212, *F 152--157). This family includes proteins encoded by 14 Drosophila genes, *F which are listed below. The presence of Psq DNA-binding motifs has also *F been noticed by Shim et al. (2001: Development 128, 4923--4933) in the *F Ribbon protein and by Couderc et al. (2002: Development 129, 2419--2433) *F in the Bric à brac proteins 1 and 2. Based on its first identification *F in the Psq protein, the authors of all the above-mentioned papers *F consistently refer to the new type of HTH domain as a “Psq DNA-binding *F domain” and to the defining 50-amino acid motif as the “Psq motif”. *F Drosophila genes that encode proteins with a Psq DNA-binding domain are: *F *F pipsqueak (psq) *F Tyrosine kinase-related protein (Tkr) *F ribbon (rib) *F bric à brac 1 (bab1) (*a bab) *F bric à brac 2 (bab2) (*a bab-II) *F piefke (pfk) *F BTB-protein-VII (Btb VII) (*a BtbVII) *F Eip93F (E93) (*a Eip93F) *F CG3726 *F CG8924 *F CG13651 *F CG11849 *F CG13496 *F *F A Psq DNA-binding domain is also present in the transposase encoded by *F the transposable element pogo. *F *F For your convenience, PDF files of the papers cited here are attached to *F this message. *F *F Sincerely, Michael Lehmann *F ------------------- *F Michael Lehmann, Ph.D. *F Howard Hughes Medical Institute *F University of Utah *F 15 N 2030 E, Room 2100 *F Salt Lake City, UT 84112-5331, USA *F phone: 001-801-581-2612 *F fax: 001-801-581-5374 *F email: mlehmann@howard.genetics.utah.edu # *U FBrf0151620 *a Manning *b G. *t 2002.11.16 *T personal communication to FlyBase *u *F From gerard.manning@sugen.com Sat Nov 16 02:30:08 2002 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Sat, 16 Nov 2002 02:30:08 +0000 *F From: 'Manning, Gerard [R&D/0111]' *F To: ''Michael Ashburner'' *F Subject: RE: where is the Drosophila file ? *F Date: Fri, 15 Nov 2002 21:29:36 -0500 *F MIME-Version: 1.0 *F X-Mailer: Internet Mail Service (5.5.2655.55) *F *F Hi Michael, *F You're certainly welcome to add the annotation and point to *F kinase.com as a temporary reference source - I'm only sorry that I haven't *F had the chance to go further on that myself. I am hoping to submit a *F shortish paper on the Drosophlia kinome, which is half-written but hasn't *F made much progress recently (the human kinome paper is coming out in *F December, in Science, and is still taking a lot of time, and we're in the *F middle of the Pharmacia-Pfizer merger, so there are lots of distractions!). *F I also haven't forgotten that I owe you some time working on the potential *F of more detailed GO assignments to kinases... *F *F BTW the CG10673, annotated as a endopeptidase probably is a kinse: *F it's a a homolog of human PRPK and yeast Bud32, all of which have weak but *F detectable similarity to the kinase domain. *F *F The other information that I would love to get in flybase and other *F databases concerns the classification and 'orthology' groups that we have *F constructed for all kinases: using the sub-family classification, we can *F often find 1:1 orthologs between genomes, and more often see 1:N mappings *F which are well curated and much better than most blast-based connections. *F The family memberships are all available at kinase.com (the human ones will *F be added on Dec 6), but we can also do work at our end to make the data more *F palatable. Have there been any discussions with the other model organism *F databases at trying to link putative orthologs? *F *F All the best, *F *F -Gerard. *F *F -----Original Message----- *F From: Michael Ashburner [mailto:ma11@gen.cam.ac.uk] *F Sent: Wednesday, November 13, 2002 9:16 AM *F To: ma11@gen.cam.ac.uk; Manning, Gerard [R&D/0111] *F Cc: r.foulger@gen.cam.ac.uk *F Subject: RE: where is the Drosophila file ? *F *F *F *F Gerard *F *F I have downloaded the fasta file of kinase sequences from yr web site. *F The following genes are not annotated as having the function 'protein *F kinase' *F in FlyBase. I wonder if I could add this annotation from your data and, if *F so, can you give me a suitable way to reference it ? *F *F Best *F *F Michael *F *F ======================= *F BcDNA:LD22679 *F CG10673 *F .. annotated as: metalloendopeptidase ; GO:0004222 ; EC:3.4.24.- | inferred *F from sequence similarity with SWISS-PROT:O27476 *F CG11660 *F CG11859 *F CG14163 *F CG1951 *F CG1973 *F CG2905 *F CG30078 *F CG3008 *F CG3608 *F CG4041 *F CG4410 *F CG4523 *F CG4588 *F CG7156 *F CG7616 *F CG8726 *F Slob *F Taf250 *F fs(1)h *F *F *F ----- End Included Message ----- *F *F http://kinase.com/kinbase/FastaFiles/ *F fly_protein_sequences.fasta 11-Nov-2002 18:07 201k *F *F >twf.AA *F MSHQTGIRANEQLAKVFGKAKNGKFRVIKVSIENEQLSCGATAETKKDWERDYDKLIGPLLEKDVPCYILYRLDAKIPLG *F YSWLLISWTPDTASIRQKMVYASTKATLKTEFGSAYITEELHATTLDECTLEGYRRHKQDFAAPAPLTSREEELKELRKT *F EVHTEINTNTRHQTLGGINCPLSEATVAAVQDLVRGKHDYLQFRIDLEEEQIHVSRAAKVELADLPKQVPEDHARYHLFL *F FRHTHEGDYFESYVFVYSMPGYSCSVRERMMYSSCKAPFLDELAALGVEVVKKLEIDSGSELTEAFLQDELHPKKILHRP *F AFAKPKGPPNRGAKRLTRPTAED *F >CG4410.AA *F MSRSAQEAAALLRGLRILLEACGREHLAHGRHLWSNSSIRELIAENVAHTQQLVRSSSQNPTEELKKLQQTVKETGERGY *F VVAKGICSLLETKIKMSREESAISEPAQVSSIRKSTPTAQNPASWDAANLDISSITLQEFEEILSRRNKDRSVSLRTPAT *F KSNQTMPKKETSTNPGIITQDTEYVNNVLRFVAGAKVEEQPPDAQPKKKSDQPAIEVPELSKVAKQRKVPSSRIGRMASF *F GGLFAGLGLGTLNELTKGALGLGGSTSMREALLSPANAERIVDTLCKVRGAALKIGQILSIQDSSVVSPQLAKAFERVRQ *F AADYMPDWQVERVMNTQLGADWRQRLKSFEDKPFAAASIGQVHRATLSDGMDVAIKIQYPGVAQSIESDIDNLVGMLKVW *F DVFPQGFFIDNVVRVAKRELQWEVDYDREAEYTEKFREMIAPYPEYYVPRVVRDLTTSSVLTTELVPGVPLDKCFDLSYE *F HRRHIAASVLKLCLRELFEIECMQTDPNWSNFLYDAPSRRLMLIDFGSTRFYRHEFIRNYRRVIMSAAENNRQGVLEMSR *F EMGFLTGYETKQMEQAHVDAVMILGEIFRYDGDFDFGRQNTTERLAALVPTMVAHRLCPPPEEIYSIHRKLSGIFLLCAR *F LNVRMNCVPFYKDIVLGKFKDYMKRKNELFHHDCVSDIDGSVSDGSEELEEQSPEQIEEIAWPQMKREIGAMNRTLDEIQ *F AQLAIDFHERDGGRSCWEEEQEALAKGLEPEDSRWMIMHGSREAALRELQWNNLRIRRQLADLVRCSELGRARISALDRL *F FQRQTKELVGCRQDHERVLSFHLTKQLEAGKCLQRYRYARDLYASWRELFKMGRHLKEAYKKILERTQSRLEYAEIKRQA *F LDEMTVAHEMCLGSTRSLLARVRDLELGLAPLGFSRPSVMSRKIGSLGTSGAGHADSNRAEEHPGRPVWVKAKAWQRRSY *F RTVKFSRGELLELPVVVPPEAKTWYGKMLRMLHNPPKIY *F >CG7616.AA *F MRPLQRVRQLLSLGTRIRGKRALNGLREANVQKGGRPVLRFSLLAAGAGGLAYDGIVNDFTYCGASVRFVRSLKTAGLIA *F ADYLRLDENDPEYETKVKLLHKKSAERLLETCLLNGGLYIKVGQGFAAINDILPVEYTSTLSLLQDRCLPTTQADVQKVF *F RKDFGQLPEEIYQEFDYQPVAAASLAQVFKARLPSGEQVAVKVQYNDLQKRFISDLGTIIFLQDIVEFFFKDYNFGWILN *F DLRKNLVLELNFLQEGQNAERCAKDMEKFSYVHVPKVHWSYTKTRVLTLEWMDGCKISDLKTIEKEKLSLKDIDVKLFEA *F FAEQIFYTGFVHADPHPGNIFVRKNRKNGRADIILLDHGLYEELPQNVRGPLCEFWEATVLRQENRMQAAAEKIGIGDYM *F RFAEVLFQQPIRNRGGGIRGKLTQEDIDHMQEIARNNFEHIMGTLKEMPRSMLFVVRNLNTVRAISHQHGDVVNRPRVMA *F RYAQKCLYMQHNRRSPVQYIRWLSGRIYFEYCLFLSAFKLYLLDWYFNILYLVGRAPASARTVMKDIMQPPEPNVLK *F >CG3608.AA *F MLLRRVLGYGVVGAGLASAGWSLHTNDYDPNSLGIVRLSRSAAAVVDVALTYKRELYYKEWDKETPEYKAEKSRVHKIAA *F EKLLQLICINKGVYIKVGQHIGALEYLLPKEFVQTMKVLHSDAPQNPIEDLYKVIRQDLHCNPEEIFDSFEREPLGTASL *F AQVHKARLKTGELVAVKVQHPYVKGNSRVDMKTMELAVNVLARIFPDFKIHWLVEESKKNLPIELDFLNEGRNAEKVAKQ *F FKKYSWLRVPKIYWKYSSSRVLVMEYLEGGHVTDLDYIRRNKIDSFAVANRIGQLYSEMIFRTGFVHSDPHPGNILVRRT *F PENSLEIVLLDHGLYANLTDKFRYDYSNLWLSILKVDRKAMRQHSEQLGIKGDLYGLFACMVTGRPWETVMQGLTKVKYS *F KEEKNTLQNNTSLVLPHISDVLEQVDRQMLLILKTNDLIRGIESTLRTQNRMTAFWVMSKCCVQSSYAEQRAKQSDSGSS *F RILWLRVRERWELFKLNCYYLYLGLINFGFLEALKQVI *F >Abl.AA *F MGAQQGKDRGAHSGGGGSGAPVSCIGLSSSPVASVSPHCISSSSGVSSAPLGGGSTLRGSRIKSSSSGVASGSGSGGGGG *F GSGSGLSQRSGGHKDARCNPTVGLNIFTEHNEALLQSRPLPHIPAGSTAASLLADAAELQQHQQDSGGLGLQGSSLGGGH *F SSTTSVFESAHRWTSKENLLAPGPEEDDPQLFVALYDFQAGGENQLSLKKGEQVRILSYNKSGEWCEAHSDSGNVGWVPS *F NYVTPLNSLEKHSWYHGPISRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRISEDPDGKVFVTQEAKFN *F TLAELVHHHSVPHEGHGLITPLLYPAPKQNKPTVFPLSPEPDEWEICRTDIMMKHKLGGGQYGEVYEAVWKRYGNTVAVK *F TLKEDTMALKDFLEEAAIMKEMKHPNLVQLIGVCTREPPFYIITEFMSHGNLLDFLRSAGRETLDAVALLYMATQIASGM *F SYLESRNYIHRDLAARNCLVGDNKLVKVADFGLARLMRDDTYTAHAGAKFPIKWTAPEGLAYNKFSTKSDVWAFGVLLWE *F IATYGMSPYPAIDLTDVYHKLDKGYRMERPPGCPPEVYDLMRQCWQWDATDRPTFKSIHHALEHMFQESSITEAVEKQLN *F ANATSASSSAPSTSGVATGGGATTTTAASGCASSSSATASLSLTPQMVKKGLPGGQALTPNAHHNDPHQQQASTPMSETG *F STSTKLSTFSSQGKGNVQMRRTTNKQGKQAPAPPKRTSLLSSSRDSTYREEDPANARCNFIDDLSTNGLARDINSLTQRY *F DSETDPAADPDTDATGDSLEQSLSQVIAAPVTNKMQHSLHSGGGGGGIGPRSSQQHSSFKRPTGTPVMGNRGLETRQSKR *F SQLHSQAPGPGPPSTQPHHGNNGVVTSAHPITVGALDVMNVKQVVNRYGTLPKGARIGAYLDSLEDSSEAAPALPATAPS *F LPPANGHATPPAARLNPKASPIPPQQMIRSNSSGGVTMQNNAAASLNKLQRHRTTTEGTMMTFSSFRAGGSSSSPKRSAS *F GVASGVQPALANLEFPPPPLDLPPPPEEFEGGPPPPPPAPESAVQAIQQHLHAQLPNNGNISNGNGTNNNDSSHNDVSNI *F APSVEEASSRFGVSLRKREPSTDSCSSLGSPPEDLKEKLITEIKAAGKDTAPASHLANGSGIAVVDPVSLLVTELAESMN *F LPKPPPQQQQKLTNGNSTGSGFKAQLKKVEPKKMSAPMPKAEPANTIIDFKAHLRRVDKEKEPATPAPAPATVAVANNAN *F CNTTGTLNRKEDGSKKFSQAMQKTEIKIDVTNSNVEADAGAAGEGDLGKRRSTDDEEQSHTEGLGSGGQGSADMTQSLYE *F QKPQIQQKPAVPHKPTKLTIYATPIAKLTEPASSASSTQISRESILELVGLLEGSLKHPVNAIAGSQWLQLSDKLNILHN *F SCVIFAENGAMPPHSKFQFRELVTRVEAQSQHLRSAGSKNVQDNERLVAEVGQSLRQISNALNR *F >PR2.AA *F MVKREEAPGGGSQVALDGSSASACSSLAAKNGASSPSKVPNNKHIIPADSISVNKQLGTGEFGIVQQGVWSNGNERIQVA *F IKCLCRERMQSNPMEFLKEAAIMHSIEHENIVRLYGVVLATDSLMLVTELAHLRSLLECLKDSGLRVSFLTIPTLCEFAL *F QICNGMRYLEQKRLIHRDLAARNILVFSKDKVKISDFGLSRALGVGKDYYKTNFNVNLKLPIAWCAPECINYLRFTNASD *F VWAFGVCLWEMFSYGFQPWAALTGLQILEAIDAPNYQRLEQPDCCPSEYYTLMMKCWQDDAAKRPRFGEIYDQLPDMKPE *F QLKAVVNCTEPKKDHLLYRQGDIISVLDRNTGTPFWKGVLSTGKTGYFNPSNTVAFLEGLPSSTRDSFSRVSDHRISKRK *F LRTEMISKPQNDFKHTGHVGIDGATFGDIAFLGSSQNYNHVPKQIVTPYKPSEDIEQTPLLLPPTPTSPDSLQTASGYFP *F EGANSGGAMGTSMNPTFIPSAEHTPKLIATNGQSSFDFASGSTNPFFPNRGDDELEFGLHNYGADGKSVHSETGWRPTSR *F SIVDDPHEYHEISDDEIAADKLDFGPSLLDEINSMFGSISAATGSHPKSPGFDHVNNKNEITEMSAKLGQKSGDTNGNKH *F GHGLLPTLSKKKSSGTVKPISVKDEKILNHAIEIANEISARSMIDLVSDQTPVIHSPKRKFSFRFPHLSNNGSGDKSGGL *F GTSGSAHTPTHGNASPFPKKKNFTEELQSIPDIQSLIGKEGLEAYNSLIERKALLDIGPSPAATLLRHLDTDEFDLQSLH *F QSQRPMTLPTRGATQRVRKAELAAGLSRHNDENSNSLEACESPSYMTHGSYKFPEAQPTEQLPEPESPNPIPLPPREGKK *F QVKTSTKRHVRKYPLIIPANGLQRTLSKLADFGDEAAKSPEISTSSQPQPGRAIENLDKLTPADAAGLTDTASLQFESIM *F EADTSKEGILQSPDVTDGFYNFSIQKEHYNKGKDAEFEATQISGLYVNDDELRNLDIESSRRTATPCSSCSALESEHSQP *F DALPSTESVSEVSRFSSVDNELAGNALFKKVRASVNMAMNRKSVAETSLTSNQPGGASAKPQTEAEYFAATAARLADSNS *F VSCEDLLEFSDKKPKGCERGVDSDEVRIMVKVLGKDVSSLCTKNQISYNQIFISQSTPNRCLGALEFINWDVHKSIKLIK *F LQNLVSEANLSLEASFEALQQHEWDLHTTAHKLNGLKL *F >Ack.AA *F MRGPLTARHQNQQPSRQLLIWREMTSTSAVDGGLGSETAWLEDLLREVQLEQFLDRIRDDLQVTRLAHFDYVLPDDLERC *F GLGKPAIRRLMEAVRKKKAHQWRKNILSKLIGGGKQPSSKKQSSAARESSQGNGTQLTCLIHEKDITMGLKLGDGSFGVV *F RRGEWSASPAGKVIPVAVKVLKSDNLTQPGIIDDFFREVQAMHALDHANLVRLYGVVLSQPMMMITELAERGSLLDTLRK *F QCRHTSLTIIWNWSVQIVTGMAYLEQKRFLHRDLACRNVLLAAGNKIKIGDFGLMRALPQEDDCYVMSEHKKVPFPWCAP *F ESLRFRQFSHASDTWMFGVTLWEMFSFGEDPWVGLNGSQILRKIDREGERLHQPDACPPDVYAMMLQCWDKTPAERPTFA *F ALKEYLASMSPPVMRASRSHHESKGLQIEPGDTIAIIDGRHELKLIKGQNQRTFDIGIFPRNLLEQRKVGAAGDVVMRSS *F VGNGSSSSPFGFCWGGAAAMANGDDRQRKCASMTNQPHAKERKSTSSKQFAYNKLVNDSATGLQRRNAVKHKGVVVGPQR *F PPPPQFQQEGILIDISPDMRPIAEAGTGGAKGAGDSSSLQADSSFCILDAPIDVPTYAGSSGSGDLNVSPTYYNEQPQFD *F FDPAKMTASPGRLQPPPYQMPPTYSNTMEFVQKRDLHQQQLATPVRERDPFDTTNVETTVALYSNFNQSLEAASPPAPIY *F NSPSVRKSLFGGSKSNKENIPALESAAMQLNLSNLTLERHDATCIQPVEPVPAPPGDGVLLDKSFIAELEKDMYSNGQNR *F AQEEYQRNSTQMYASKDMVYKQNLTPLKNGAAPGSVHSNHSSPSSTASPKQNNVEAAAAAAATTQSVVNRIWYEQVASTQ *F SEYYAQPPTEQAEEQIYQNHRHQQQQQQELNHSFVAISNRVVAPKNNAYSSTASLYDAVAASTAGSTYYGQVPNGSGAVL *F YDEVTQDDYLRPTRPAPLAPPPLSAQQIQRRMEKMRLQQQQQLDGAHQLYAPVPSDYGREQEKLQQLMQELGSSAVEQDV *F RNALRAASGDVGLATRHYKIDQLARLGVAGRPQCEQALQQTNWSLEVAAELLLNAG *F >Akt1.AA *F MSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVR *F GCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDE *F LSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKST *F NHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDG *F HIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEV *F KFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGES *F VELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ *F >Alk.AA *F MPQLRQLVVVLAILSLLVDPSFAQRPLIMNHTFSSLPMSQHPSFNSWPSASGGKQLRNGRLHEQPLPLPQPTLPVATQYE *F DYEAAPPGSRRDNKRRLAQMAQRPGSGNGGRRGMESLRKELMNPSVGGPGGGISGGGSGYPSYSATSSIGRIDGLGQGLG *F SADRYGALYDQLRQPSGMAAMTAGPLGGPYSTRFPPLYAGVDDQPKRSNRKNSISELYKLKKALNQADEPAGGVTHGNFE *F GDPQPSSADPLQEIKDRIRDTSPNANTYAPHTDATPSSEYEYELGVKCNFETPCSWTWGNYSDGFQVITGTELSKRNLTG *F LLPGPAADSIDDANGHFLYARVNPSSRPLNLTSPEFSTTMEKCFLEVYMHQSDMSHGLSRVVVELLHTAESSWVPAEILG *F DNVRQWTRKVYRLGRVSRDFRIVFEVVPDLRVGQKGHVALDNLRMVNCFPEGTKSEKCSTSQVKCTSSKVPVCIHLPRIC *F DITRDCDEAEDEQQSCDKIPYGGRCDFEEDWCGWRDSGKTTLTWSRHTGSSPTHDTGPDGDHTMQHLQNNTSGYYMLVNM *F NQHMNNSEKNSIIGFASNAIMVSKTFNPPPSVHGNPDSPYRNSCVVRFFIHQFGKNPGSINLSVVEMKEKENITTTLWWS *F TKNQGSDWMRAEYVLPNITSKYYLQFEARMGMRIYSDVAVDDFSLSPECFGLNIPEDHLGGYNYWDVRQNLKSPTYKDFE *F YTNYLELTTCDTRGMIGPSQAQCEAAYREQNKTHVLREVHVVEDQSSYKGMQKWKVPHEGHYTIIAKGASGGLGSGGVGS *F SRGSVAVAILELHKNEELYFLVGQQGENACIKSMGVLKEAGCGTDHDLDLAQYSFRSKQDMVKNIYIENGAGGGGGGSYV *F FLLNQAKNEAVPLLVAGGGGGLGIGQYIDEDFQHGQKAKPLQAPESGQINGEPLNKKTAGPGGGWRAKEDQALSPTYGAA *F LLQGGRGGHSCYVELADNGTSVHRHGQGGFGGGGGGCNTGGGGGGYAGGDVYLTESNGEGGSSYISPSRSLREISEIHAG *F ASSGPGAIIIIPAIEGCGCDYRCVALDEFRSKVRCICPDGWSLKRDNHTACEIREEAGKSSFQYLVSILMISLAVLFICI *F AALIFMLYNRYQRKKQSKKRHKMLVEQDLQLTRLRNNIDDSNLNNFNPNYGCDGILNGHIDVNSLPQVARDSLQLVNALG *F KGAFGEVYMALYRHRDGDAVEMGVAVKTLREDPKREKEEDFLKEAAIMAKFNHPNMVHLIGVCFDRQPYYIVLELLAGGD *F LQKFLRENRNTPERPSLLTMKDLLFCALDVAKGCRYMESKRFIHRDIAARNCLLSSKGPGRVVKIADFGMSRDIYRSDYY *F RKGGKAMLPIKWMPPEAFLDGIFTSKTDVWSFGILLWEVFSLGRSPYPGQHNTQVMELVVRGGRLGSPTECPVSIYKVMA *F DCWNPTPEDRPTFITLLEHLTACTQDASIMNAPLPNILGPTASERDDTVIRPPNGEEFCLAVPDYLVPLPPGGSNNPSMA *F SGSGYVPELQRQQMSSCTPPAVTSPAAPHPRPVENIAPTSGDCWETSFILPNSKVEPPVVGSGHDVPLAGGEEAKLISLD *F TPQPTPTTIQPPLSFASQLDGITLDPSALTKSLPNGNAKQSYANVQVKNEAEAKVINGVVGNGTVMNGDVSTGSGGADSP *F FTIQGYAERYKDNNNHSEISC *F >CG30078.AA *F MYFETNWVFHQPRSWIETNFQKRECIKFIPCPKDDTKCCCGQAQITHQTIPGIESGSPGDLWLPTKHTRPQPTDAYGTIE *F FQGGAHPTKAQYVRLSFDTRPELLVQLFTKEWNLELPKLLITVQGGKANFDLQAKLKKEIRKGLLKAAKTTGAWIFTGGT *F NTGVTKQVGDALLLEGQQRTGRVVSIGIAPWGIVERNHELLGHNREVPCHSISSPRSKLAVLNNRHAYFLLVDNGTQAKY *F GAELILRRKLEKFISNLKLHPFTHSSTPVVCLVIEGGTNTIRAVLEYVTDSPPVPVVVCDGSGRAADLLAFVHKYASDGE *F EQPVLESMRDYLIGTIQKTFEVGLDQSEKLYQELLQCTRNKNLITVFRIQEKPEGEAQELDQTILTALFKSQHLSPPEQL *F SLALTWNRVDIARSEIFVYGQEWPNGALDEAMMQALEHDRIDFVKLLLENGVSMKKFLTIPRLEELYNTKHGPANTLGYI *F LRDVRPHIPKGYIYTLHDIGLVINKLMGGAYRSYYTRRKFRPIYAKVMNSYANACRKSSTYQYQRYAGANSLSLVTGLLP *F FTSEMALFEFPFNELLVSGFRHNTHPSCCTSRLLFFKIWAVLTKRQQMALLMWTHGEEALAKSLVSCKLYKAMAHEAAED *F DLDTEIYEELRSYAKEFESKGNKLLDFSYRQDAEKAQRLLTCELHSWSNQSCLSLAVAANHRALLAHPCSQVILADLWMG *F GLRTRKNTNFKVILGLAMPFYIRQLDFKSKEELQQMPQTEEEHLENQNLDNDDSDRSQPDAEVSLTDSDTAQFREFFNLS *F EYNEVKQHQPLRLKKKFYEFYTAPITKFWADSIAYMFFLIMFSFTVLVKMEQMPRWQEWYSIAYITTLGFEKVREIISSE *F PVAITHKFSVWAWNMWNPCDGAAIILFVIGLAFRFRENTMDIGRVIYCVDSIYWYLRILNILGVNKYLGPLVTMMGKMVK *F NMIYFVVLLAVVLMSFGVSRQAILYPNKQPTWSLIKEVIAGSITAPGFLGALGHNTRSSYHRGYHTMGAPPVTTTAASTT *F TGSSSTVPAATTSTTAPIHNEHSNLTSGNLTNVTFQPYFMLYGEVFAGDIDPPCGEDPSQPGCVTGHWVTPITMSMYLLI *F ANILLINLLIAVFNNIFNEVNSVSHQVWMFQRFTVVMEYQQKPVLPPPFIALCHFYSLLKYCVRKAKGLEVQRDNGLKLF *F LEKDDLERLYDFEEECVEGFFHEQEIILNQSTDERVKNTTERVETMSQKIEDINQKENIQTATVQNIEFRLRKMEESSEQ *F ILSHLAVIHRFMSTHTAGADDLRGSTINIPGEMQRMRTISISDTEGGSGPGGNGGGGGGGGAIVPLGLGAGLNLNSLQVT *F TRRRFNRSLTEVRPDAYIFDEGTHFEVVPLPEEPDEVVKSREALNEQVVRKASMQSEADSDIYIPVSQRPSTCETVKRTP *F YVTVRQDTGASTESKDTLTPMGNNDDDQTLVGGDNSDDATPDINFEAARHRALRQRTVSLCRRNSETYSLTGADINRSHI *F SLNQLASLSRRQMSLTQSEPDSDKDAPIAQGSAHPGKSVLHAKPSRNILLKLHSEYTSITDELESVCHMIASPTVSLPSN *F KASLDRPKTEMSRAEAAALLEKKHLKECEENDYMILEGLIESRGSIDASAQGFEIGVSIDYSHRYPLRRETAVELSPSKP *F SVDGDLMGGGEGGGAGGGDSSDTSGAGSCGAMVGISSGFQLKNERPWQRNSSMEQQTYPSPLVPTRATSDFLNPPYEGRL *F FKKSSESLQKNSSTETDYSAHPYRFIKQSSNETNTSLTGSYNVDTPSLTAEPSLDAGDSHSATGISISVGAVGGTATARY *F QPIRTASVGAADGRRLREESSSSLDLSSSGPVTMQAAPAPPVRPMLLKKQFSVDQGKPSQTAAEAVPQTPEAAQAGQAKL *F ISTLKPQPFASKLGMNVLKESSSSTDESVGSSAKSSNPALSIPQISTHLVQDEIAKLSSNIKSSTESEKDPPFNETMC *F >ial.AA *F MTLSRAKHANRNHLPHLLAKVPEEHQEPIKNMCLKMMSHDAYGQPYDWSPRDFEMGAHLGRGKFGRVYLARERHSHYLVA *F MKVMFKEELRKGCVQRQVLREIEIQSRLKHPHILRLLTWFHDESRIYLALEIASEGELFKHLRGAPNHRFDEPRSAKYTY *F QVANALNYCHLNNVIHRDLKPENILLTSTDDLKLADFGWSAHTPNNKRRTLCGTLDYLPPEMVDGNSYDDSVDQWCLGIL *F CYEFVVGCPPFESNSTESTYSKIRRMEISYPSHLSKGCKELIGGLLRKESKGRITLVDVMTHYWVKAGMAERELQLQKRE *F RGKENTARN *F >aur.AA *F MSHPSDHVLRPKENAPHRMPEKSAAVHNMQKNLLLGKKPNSENMAPASKPLPGSSGALIRKPGLGGSNSIASSEGNNFQK *F PMVPSVKKTTSEFAAPAPVAPIKKPESLSKQKPTAASSESSKELGAASSSAEKEKTKTETQPQKPKKTWELNNFDIGRLL *F GRGKFGNVYLAREKESQFVVALKVLFKRQIGESNVEHQVRREIEIQSHLRHPHILRLYAYFHDDVRIYLILEYAPQGTLF *F NALQAQPMKRFDERQSATYIQALCSALLYLHERDIIHRDIKPENLLLGHKGVLKIADFGWSVHEPNSMRMTLCGTVDYLP *F PEMVQGKPHTKNVDLWSLGVLCFELLVGHAPFYSKNYDETYKKILKVDYKLPEHISKAASHLISKLLVLNPQHRLPLDQV *F MVHPWILAHTQ *F >fs(1)h.AA *F MSSSEPPPRYEPPVEPVNGIVQPPVIPPAERPGRNTNQLQYLIKTVMKVIWKHHFSWPFQQPVDAKKLNLPDYHKIIKQP *F MDMGTIKKRLENNYYWSAKETIQDFNTMFNNCYVYNKPGEDVVVMAQTLEKVFLQKIESMPKEELELEPVTAKGGKKKQR *F APATPKSSSGGAGASTGSGTSSAAVTSGPGSGSTKVSVAASSAQQSGLQGATGAGGGSSSTPGTQPGSGAGGAIAARPVS *F AMGGTVSSTAGGAPSIPPISTMPPHTVPGSTNTTTTAMAGGVGGPGAAGANPNAAALMASLLNAGQTGAYPGAPGQTAVN *F SSSLLDGSTAAVAAAAAAAAAAAAAAGGAAGAAGGAGTIPAVAVNAANAVQAYVNAGVSVGVDAVIPPQQPAKIKKGVKR *F KADTTTPTANAFESPYTQMDSKSAKIATRRESNRQDLTFQGSGYNMSPLGVSGVPGLGGLVAGGVAGVAVAKNKEKLSDA *F LKSCNEILKELFSKKHSGYAWPFYKPVDAEMLGLHDYHDIIKKPMDLGTVKRKMDNREYKSAPEFAADVRLIFTNCYKYN *F PPDHDVVAMGRKLQDVFEMRYANIPDEPVANAAHHHGHGHGHGHGHGHGHGHGHGHGHGHGYGGSSSLKHDASDSSSEDS *F SDTENESNSDEERSARLKMLESKLLGLQEEIRKLSEEASAKKKAKKKLKEKKKSIGGGSGSGSASHHCHATGGGANAGGA *F GGPGSGGHGSVSVPGGVGSLGPGGAGGANLNALLGGSLVGHGGAAVAGGVPNVGALHSQVHDVAMAFSQMAGGGAAAGAG *F FGAGVTAAGASSGGKAGTLAGALAAGAAAGAGGTTAGSGSSKGAKSKGGRGAKGSGAGGVGASNNAAAGNAAGGAAGAAA *F GAGSVGGVGGAGAAGGGNASKRAKGSSSAGAGGGVGGANASAGGAGARGSSKKKPSQVMNFDSEEEDTAKPMSYDEKRQL *F SLDINKLPGDKLGRVVHIIQNREPSLRDSNPDEIEIDFETLKPSTLRELESYVASCLRKKTRKPYYKKPSGKSKDEQMAE *F KKQELEKRLQDVTGQLGASKKTAKKANMLGNANPLTAAAMLNNNNKTSLPGSNFGGAPAPGNMMHAGAGVPVAGAAVSAS *F TGQQHNKNGPNDLSKVQPGGPINAALPPHSFAGGTATVATSQSSGGIRIASNLHKPSGLGGGDLGEHHAALAAALTSGIN *F STGTAGGGINNNGGSNNNANPLGGSHGDAMVNASLASLASGLKQIPQFDDPVEQSLASLEFSAGSTGKSGLTDNFLMQQH *F LMQPAGPQQQQQQQQQQPFGHQQQQQQQQQQQQQQQQHMDYVTELLSKGAENVGGMNGNHLLNFNLDMAAAYQQKHPQQQ *F QQQAHNNGFNVADFGMAGFDGLNMTAASFLDLEPSLQQQQMQQMQLQQQHHQQQQQQTHQQQQQHQQQHHQQQQQQQLTQ *F QQLQQQQQQQQQQQHLQQQQHQQQHHQAANKLLIIPKPIESMMPSPPDKQQLQQHQKVLPPQQSPSDMKLHPNAAAAAAV *F ASAQAKLVQTFKANEQNLKNASSWSSLASANSPQSHTSSSSSSSKAKPAMDSFQQFRNKAKERDRLKLLEAAEKEKKNQK *F EAAEKEQQRKHHKSSSSSLTSAAVAQAAAIAAATAAAAVTLGAAAAAALASSASNPSGGSSSGGAGSTSQQAITGDRDRD *F RDRERERERSGSGGGQSGNGNNSSNSANSNGPGSAGSGGSGGGGGSGPASAGGPNSGGGGTANSNSGGGGGGGGPALLNA *F GSNSNSGVGSGGAASSNSNSSVGGIVGSGGPGSNSQGSSGGGGGGPASGGGMGSGAIDYGQQVAVLTQVAANAQAQHVAA *F AVAAQAILAASPLGAMESGRKSVHDAQPQISRVEDIKASPGGQGQSSPAQQSPQDRAAAKRAEQRRAEQERRRREALAGQ *F IDMNMQSDLMAAFEETL *F >CG14030.AA *F MAMHSYMRQGSGSGGGAGAVAAGAPPLASPDEMHGFLNDKQAWEHAISLYQGPDPLDHWYNYICWYENHAQSDPELKYRE *F TLERCLTVYEHNDYYRQDVRLVRLWLKYIAMQTDPLHFYQVLFQRGTGRQVAAFYIGWAAYYESREEYKDAEAVFNLAFQ *F EKAQSTSELQHAHTKFAYARSLFYQRQQQQQQQQQQHQQHPPQDALQQLTNYAQQQMPQSYNQHRPQPYQQNVYQQYHPQ *F AQAHQAPQPHQPAPQQQLPPEQQVPYQTHYQERPRYEPHPATQSPTAIPPSQVQQQSHYAPVAESHYAPAQQSQLPPQQT *F TVPQLHAQQPQQQQNGNGNPPPQQSPPVTNEVAGLRLPRNFHAYGRNNHETWKPALTLEEPDDPSRVCHYAKQLVYPPGA *F GVEYSPEEILARKFKQLMDQKAKPSEPPEQEQQTLYDSYETEKSYYMTAVDGALYGQNTSSGQENTGEEDEDNDAEEGEE *F EEDGGEENEEDDSDEEEEDDEEEEEHSGPYTNGVQFSAQTTFEQENRSIKIKFRKEPSSTYSAYTIENVYQQQQQQQQEQ *F HQIIHQPPQAVHHPSPDPAPASPIPIQRQRNGSHHHFHPYMLGQTSTPKSEANGYRRARTKVKRSKFQPDLCSNSNSASS *F VADVASSSVLAGAPGTFNDNANFSFSSATALDNSNSSLALAVDRLNFRDTSQQQILHPVNKTLQIHNNNNNTSNNNNGTS *F TMADFSTFQENSYFATQHDTEAQERRLSKAVETIARHMDKEAIDPFNSELCRAFLAKLDFPGNHDAHASYKIVQTPLPKI *F SNTRTLNVLEGVTFSIDKEVGRGSYGSVYKATDSRTGNVVALKYQKPPNTWEIYICDQVLKRIKEPEVLPGVMDISTAII *F APNASLIATEFSPFGSLLDINNKIRQATTKVMHESLVMHFSAQICNIVDHLHRQHIIHADIKPDNFLLMRVPNVDSPLPS *F LRLIDFGCAIDMTLFPDGEKTKFRKVVQTDGFTCIEMQEGRSWSYETDLFCIAATVHVMLFGDYMQPQKKGSSWEIRQKL *F PRYLKKHVWTKFFGDLLNMQADKLPALHEMRLIFEEEAYRMDSELQKQIRTLSNILHRR *F >Bub1.AA *F MDFDNAKENIQPLASGRNVSLLQASLSQDSTHGQELLAQRKQMEEEVRTYKGADPLGAWYTFICWIEQSYPAGGSGSGLQ *F TVLHQCLTKFEDDERYRQDKRLIKLFIKFMEKQKDKIEFYQQMYNNGIGTMLADFYIAWAYSYDLSGNMRKADEIFRLGL *F ECRAEPLEDLKEAHHHFGYTVGQRMLYSTGEEANAVNQELNERRLALQSLHGRRQQISNSITVGSIRTGAAVKSGLPGVV *F QVEPSTTTSRRNVGRNVEVFNDENSEGNIPISNSETEVKSSLRSIIDSACSQENIKEPVAWNKANAKRHKHGKIFGSNAS *F PDLGFDIHVDEQAMPPITNYERRLEQPFRFPTNFVAKNRPQEPWVTPVTIEDEPNASGLPCYNKCLLYPRPNIEFSPEEY *F RAYSFLKHRNPQHPFIHRNDDWWGTGRVIRGIRCYPNFARYSKPQDLDELDKFWKPPLVPGLQVVFDKIYNEVEQKEYQP *F EELLAAKWMEKRNVTVHGDFDMEETVCLPGNKMPRRKSFFPSSFRKSIMPRRVSSVQEEDEKKEDEATLIVKDIPSQVAP *F KIPESPCDQIDEPKIKIFEDSGSPRDNFAVPAVPVPKIEIYEDSEEPQPTPKTRPFYDGDETCSTQMFNMFIKSQAVSTP *F KGTQKQAPSRQFGTVLKELPLPEEAAHSVESPVETRSPTLRKQLSTILETSEHGTQSLATSGTTTKSTITSTSSPGSNAV *F SKLGSKIEENTPGQMRLQRVISAGVSAVLSEPEKPGGDNFLRRELWEPNAPSVPMLKSLRFQEDKTETIPRPLACFQEDK *F TETLPQMPIALPENSMDHGSLGAENCFSAPQLPTLEDESDLCGLFGKTPPKTNIFGSPKRNFDPTSQLFKSTQGDRRSFA *F VTGNKLEAAPSISNLQDSFMCDLSFVPETQPEAVAKSGAIQKFDIFLDDTQPKLTKSKPVALMVSSFDLDCTLPETQQVA *F IKEPGTQQENIGHSHMIASFMKDCTEIGSCPSQMPASVVKTNISSTSNKIKFSNDSMSESSRKAPVNENVGISKQPDEFF *F ELNAATEMFATNISMIKNSTLLIPNAKVAEASELSIYYKTTPLTPKQSHRSWSQSDLETPPRENFVHPTSNGDQTVLNET *F LADANKNPFNVELISSLLESIDFSMYIEKLPHCQLVGHVKRLHPNTHLEVHNEKFEVSKMIGKGAYGSVYVGKHLKSGKK *F VALKQERPTNYWEFYICLEIHSRLTSEQMIPSYAHIDYALVGNNSSVYISEFSDYGSLIGVCNKVKSVTNRNMDEYVVMH *F LSCQMLDIVDHLHAMGIIHADIKPDNFLLMKPICADPNEVSLQLIDFGVSIDMKLFPDNQTFNYVHHDDLFKCIEMRTGR *F PWTYQLDLYGLVSVMHVLLFGRYMEVVQRSPSTIWMPKTNVPRYFQRTMWENIFRTLLNIRDCRTMPNLQQLRTQLKCAL *F AEKEKYVAEAISKFNTILQK *F >CG10673.AA *F MSLEILKQGAEGRLYLGDFKGEACLIKERFVKKYRHPELNTQITRQRMKAEAKASGRCLAAGILAPKILHSDLNTHKLYM *F EYFDAAKTAKQFIQETVSTQTEDEAKKCLLEFCTRIGEIIGKMHSNHIIHGDLTTSNILINPKGGDYDVILIDFGLSHYN *F QATEDKGVDLYVLERALLSTHSEQPYIFEHVLAAYRTACGKDEQAVLTKFEEVRARGRKRTMIG *F >CaMKI.AA *F MPLFGKKDSGKKAKAKDLKELNKQVSIEEKYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKALKGKEESLENEIR *F VLRRFSANHFDGKCLNGTRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVDYMH *F EQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMEDSGIMATACGTPGYVAPEVLAQKPYGKAVDVWSIGVISYILLCG *F YPPFYDENDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWISGNEASSRNIHGTVSEQL *F KKNFAKSRWKQAYYAATVIRQMQRMALNSNSNANFDSSNSSNQDSTTPTAATGAWTSNVLSSQQSVQSHAQEMNKSGGST *F NAASQ *F >CaMKII.AA *F MAAPAACTRFSDNYDIKEELGKGAFSIVKRCVQKSTGFEFAAKIINTKKLTARDFQKLEREARICRKLHHPNIVRLHDSI *F QEENYHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQILESVNHCHQNGVVHRDLKPENLLLASKAKGAAVKLADFGL *F AIEVQGDHQAWFGFAGTPGYLSPEVLKKEPYGKSVDIWACGVILYILLVGYPPFWDEDQHRLYSQIKAGAYDYPSPEWDT *F VTPEAKNLINQMLTVNPNKRITAAEALKHPWICQRERVASVVHRQETVDCLKKFNARRKLKGAILTTMLATRNFSKFTGR *F SMITKKGEGSQVKESTDSSSTTLEDDDIKAARRQEIIKITEQLIEAINSGDFDGYTKICDPHLTAFEPEALGNLVEGIDF *F HKFYFENVLGKNCKAINTTILNPHVHLLGEEAACIAYVRLTQYIDKQGHAHTHQSEETRVWHKRDNKWQNVHFHRSASAK *F ISGATTFDFIPQK *F >CG17698.AA *F MDLTDPLLITKSQQVNSQEVDFQKKTEDTFPSKFHKNKASCGLLAVNESRCTDHQLQCENNIVGLSLETQIAENSSINVS *F RELKPHDHTFPKDKQTFKQISDELRKSYENINKVQNIKKSQSVENFEKCSEINLANKCLYTNLNTSPKERNALDKQAMFI *F KFHSLELCTDKVCSLHIHENFCTDIHKCVVDSESNKPNKMKKCLDYESVKRYPCKVSNEPPQHNTASKPQILYETRPIYP *F NVPYSPYSTPFSSPRSSRRKPAFRESRRISIDKSGSFLQLNQYRLMEQIGQGSYGLVKLAYSEEDSTHYAMKILSKKRLL *F RQAGLMRRGPRKATSPLDRVYREIAVLKKLDHPNVVKLVEVLDDPLEDSLYMVFELVKQGEVLRIPTDNPLSEKRAWSIF *F RESLLGLEYLHHQKIIHADIKPGNLLLTEFGHVKIADLGVCNEFLGDDATISNGSTAGTPAFRAPETLIPGQNEYCGRAA *F DVWALGATLYSLIFGNVPFLADSVPLLYEKIKQDSVKFPENHKVTENLKSCIVQMLEKNPTQRITIPQLKTSKWVTSDGD *F YPLPTEEENCCLVQVDDEDIDSVVRSIPKLDTLILIKTMLKKHSFGNPFVKGCSGTSSSLAGCSRIERFIRAGRSNSAPG *F SYHMSTARKKHSFGNPFVKGCSGTSSSLAGCSRIERFIRAGRSNSAPGSYHMSTARQPSSDTLLPSLMEHCSTQCNEGTH *F SFDY *F >CG9374.AA *F MTVTTMEAQVKAAHHHHLHHPTGGGAQHKVEEEEPDPVEDEMTVLLANKNFHYDVASDLDDDAFVELKEDTAQADDAGAG *F VGFYNPDELLLDDQPHPQVTWLDDDEIETLDRVTLDMGNMFFNRVDSQDIIYQQKKKSIKMVGKYIMGDVLGEGSYGKVK *F EAMNSENLCRLAVKILTKRKLRRIPNGEQNVTREIALLKQLKHRHVVELVDVLYNEEKQKMYLVMEYCVGGLQEMIDYQP *F DKRMPLFQAHGYFKQLVDGLEYLHSCRVIHKDIKPGNLLLSLDQTLKISDFGVAEQLDLFAPDDTCTTGQGSPAFQPPEI *F ANGHETFAGFKVDIWSSGVTLYNLATGQYPFEGDNIYRLLENIGRGQWEAPAWLYEMDADFANLILGMLQADPSKRLSLQ *F EIRHDTWFRSAPVKTGPPIPIPPLKGDKYRNSTVIPYLEAYHYGTQEEDVYFTEHDVNQELARQAAAAASEIRAKQSAAA *F LAACHTYEPPSTSAAAASNSLGNGSREEAPVKKKGSALKRRAKKLTSCISVRKLSHCRTS *F >CG3105.AA *F MEDQGDVTDQAAGKLSSSNAQCSRNRCANDDPIMGSHSQSRQNSSGMLNMMDASSFSMPPPNLHSSKVINPNKAIFTIDA *F NTGQIFIVNNKACQLLGYTSQELRNKGFFDLLNGKTESHISSLAEMQIEGDEGRVVLLSGKVIEMKTKSGGKILVSLWIR *F QISSDGRHIAVAEPVERHICHISTDRSGVITSVDSTTATIFFYESVESVVGVSIVTLIPFIKLPDPDSREIPKSLRKQRA *F TGRTTDNVKFPLCLLIALDEDASAGYSHSGKTGLNITVWVFQNLSGLIVVDDIGNILMCNQPFSLLMFGYGQDKIMNMHI *F SAILPNFGKDSREEKSPNVSNTSITSNDWEPDTDPLVVDNDSSLQSCKKSSTNRTAPNNEQSSVGDAHLSCNLENSSGLF *F CDLRQPDDCTIDDILTPVNASNSFPADEFEVGSQSQVNESSLDKAKSASTETCDASGSNPATRLLSSINGSFVGEAIHAD *F GSVIEVVYSVLLQILPCSNRVYCIWVCRNPSTRLDGEKYNYANLTSTFNSMASTVEQSLGQVIKTTAAQNSSRPNSLSLV *F SKYEDELYLGDYSKYYTSIRQIGRGAYGYVNMAFRNSDRLLVITKFILKEKLCSQFMVKSRDCKEVPIEIHLLQTLNHKN *F IVSVLDVFENDLFYQLVMEKHGSGMDLWTFIERRPLMDEKLGSYIFRQVADAVNYLHEQKILHRDIKDENIIIDQNFTIK *F LIDFGSATFMEEGKFFSTFYGTTEYCSPEVLAGNRYVGPELEIWALGVTLYVLMFFENPFIDVEETLKAEIQIPKAVSEQ *F LSRLLSSMLNKDPKYRCTMHQLITDPWLTQEVNPSTFSFSWIVPCKAHEANPNLYFSGYLYSSTSVLSTISPQESFSHIE *F ESSIGGSDDARLASHRTGHKLCVNEGTILLRLLRILVVKHPFVILAKHNKMDTLYAKQFQLNTSVSNHELRVSLPDSSHT *F EIGGSICSSKSENDIFKNKLQPCVSTYNVVSLHDVSTKPKKLDLK *F >CG11870.AA *F MVISKPDGTAPNGAAGGAEAAAPTGLDATGNSLAHPSGIPQDQIDNIMSGIANTGNVKMNNHRKKLRQRKVAPELIESIL *F KFDIIKKLGQGTYGKVQLGINKETGQEVAIKTIKKCKIEAEADLVRIRREVQIMSSVHHPNIIHIYEVFENREKMVLVME *F FAAGGELYDYLSERKVLTEEEARRIFRQVATAVYYCHKHKICHRDLKLENILLDEKGNAKIADFGLSNVFDDQRLLGTFC *F GSPLYASPEIVEGTPYQGPEVDCWSLGVLLYTLVYGSMPFDGSNFKRLVKQISQGDYYEPRKPSRASTLIRDMLTVCPRK *F RASIEQICSHWWVNENDNVSCLDLAEDLANQTPVRLDVLLSLTPATITADQLVVPSAEGAAAAKAAANERVPRSHSVGSI *F RDMGPPNTEAERRILDMVAGESMSILLLDASEIA *F >CG15072.AA *F MATTPTAGPAAAPPTSSTPQNYKVPSTSKISVDKLLRVGYYELEKTIGKGNFAVVKLATNIVTKTKVAIKIIDKTCLNEE *F YLNKTFREIAILKSLRHPHITRLYEVMESQSMIYLVTEYAPNGEIFDHLVANGRMKEPEAARVFTQLVSAVHYCHRRGVV *F HRDLKAENVLLDKDMNIKLADFGFSNHYEEGATLKTWCGSPPYAAPEVFQGLEYDGPKSDIWSLGVVLYALVCGALPFDG *F KTILELKSRVVLGKFRIPFFMSQECEQLIRNMLVVEPDRRYTIKQIIKHRWLSEWQSEMQEQERFGDMSPGSGTVSKSAS *F TSSLGSASDSPPQLDSVVMTHMLQLPGLTADMIAQSVHEQRFDNIYAIYNLLHDKLQQRRRENQRLQHHASLAYSRSRKT *F SITTGVVDRSEPVKQESLDRLSPLSNANASSSALGFGWSDVAVDLEKYGDFELECLARSNDPPVNAQHLSAHAGGGVNGA *F NTRRHTVGPGDVAHEQALANPNVPPIDFKCPPQCSDPTQPVPYYPVNLPMLQNQPLHNLTIKDQHLLKPPVVMGASSFGR *F RASDGGANLHIYYPATGTVVGPAQGQQMDTAGYYINPNCGTDPLAVQELSPLNEQSVAQMQCCQENATGECNEELQSYMQ *F KRGGTQRHTVGCTEDLSVAHGSGAGSQSQAPTTSSNMRQRRTGLLTVTERPPGALERLTKIL *F >grp.AA *F MAATLTEAGTGPAATREFVEGWTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHI *F LRFFGKRSQGSVEYIFLEYAAGGELFDRIGEEPDVGMPQHEAQRYFTQLLSGLNYLHQRGIAHRDLKPENLLLDEHDNVK *F ISDFGMATMFRCKGKERLLDKRCGTLPYVAPEVLQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDH *F WQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLS *F NGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYF *F QRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGL *F EFKRRFIKIKNALEDIVLKGPTTWPIAIATNSVP *F >CG8485.AA *F MTLETQPVGGVSDGKIAGLYDLEETLGSGHFAVVKLARHVFTGAKVAVKVVDKTKLDDVSKAHLFQEVRCMKLVQHPNVV *F RLYEVIDTQTKLYLVLELGDGGDLYDYIMKHDSGLSEELARKYFRQILRAITYCHQLHVVHRDLKPENVVFFEKLGLVKL *F TDFGFSNKFLPGQKLETFCGSLAYSAPEILLGDSYDAPAVDIWSLGVILYMLVCGQAPFEKANDSETLTMIMDCKYTVPS *F HVSTDCRDLIASMLVRDPKKRATVEEIASSAWLKPIDEPDSTTSTSEHFLPLVSREQLGEEDHAFIIQKMINGNIASKEE *F ILQALDKNKYNHITATYFLLAELRLRRRREELAQKQRLLNDASMKVGDASRKLVPEKPSPTEAQKGGVPISINVTPAAQF *F ANDGGKPDKRSRKCSIVREEDEEESATECTAVGNELKVTSSSRREAFSDSPFSRSIHERSSSEPGNQTKGEQIANSGVPS *F HSRTKIVVSVDATLAHKLKQMEKSAKIDEDTLSGLKELEIGKLKPLPSSSKNPVLTHRRTKLNKIRTPSCSSSEASDDDT *F KTRNKKKINKFVGDTPVRFRMHRRDSHDDSSDSQDQLYPPPGSASNAGNFISKSGSVSGKKEGQSQYKEPNNKSDENRKS *F HSQKNRKQHNKDHVDKHSHAERQLADSSTATTGNSTNRRRRIRESQSLDRITEAQEYEMRHRCYAESEAASSSSSSTQHI *F DQRYSLLSLNTSTFSVAETKEEYDEEADATNATDQIMNTLNAAKATLQQEQYFNNNTNLSRNFNHNHNQSIIHISSQKAN *F GKKSNETPKDIYNLNSIEHIDLILEDKSLTKAIHVTGSKCFVTMKKIRRLGKYFPVMNFS *F >SNF1A.AA *F MPQMRAAAAEAVAAGSANGQPLVKIGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNL *F KLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLD *F HNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKS *F GIFPIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKHEWFQKDLPAYLFPSSIEQDSNVIDTYAVAEVCTKFGVKETEVH *F NSLLSGDPHDQLAIAYHLIIDNKRFADDAANQINEINNFFVAGSPPPPPPPPVPQSSMDHQAPLATVTVGGGTSASSGTA *F TPVPPVAGGTPSSTIPIRPHPERIAPMRDRQLAMSVQTSGGGAFPEKTARGGTPIKRAKWHLGIRSQSKPNDIMLEVYRA *F MKALSYEWKIINPYHVRVRRQNVKTGKFSKMSLQLYQVDAKSYLLDFKSLTNDEVEQGDDVIMESLTPPPLSVSGVMPLQ *F PTGHHTMEFFEMCAALIIQLAR *F >EG:22E5.8.AA *F MSTCEAAAAGENGSQAKSEESQPPEDQKKKQPQHERNEKQLDKPPENLPQNGKTEAKGAEGACSHPPLDALRSSVLLDAG *F AASPSIDAIVACKDALLAQKLFASGGGSTPGPSPTSSAVGAGGISGKDLLKLKEPMRVGFYDIERTIGKGNFAVVKLARH *F RITKNEVAIKIIDKSQLDQTNLQKVYREVEIMKRLKHPHIIKLYQVMETKNMIYIVSEYASQGEIFDYIAKYGRMSESAA *F RFKFWQIISAVEYCHKKGIVHRDLKAENLLLDLNMNIKIADFGFSNHFKPGELLATWCGSPPYAAPEVFEGKQYTGPEID *F IWSLGVVLYVLVCGALPFDGSTLQSLRDRVLSGRFRIPFFMSSECEHLIRRMLVLEPTRRYTIDQIKRHRWMCPELLEHV *F LIAKYNLGAERQTSVEPSEDILRIMAEYVGIGSDKTRASLKKNTYDHVAAIYLLLQDRVSHKKEQSNGLGASALASSTSA *F SRMIYSSRNDHQPTQQQSQQQSKTISTSSILAKDQCHKRLSRHQTVLMSERNAHAGATPTVPDPGPGYYAKYGPLQLPLP *F LTGHSHLTGYLNGGGVEVDASGIPLPMRYTPLPTAASPAPSNCSSTSSRVGRHSLSSSSPRSHRPVAISLSIDNNPSLAN *F LRCREMMEAGGGPVGAVGVPLASKQLHQTISEFIIKQSTEDCRALLQQSTAVAEGKDDPPKAESSVGGVPPPASTTPTSS *F TAGPESGSAPCPGEINGKTIKTMSSSSSFDSKANLGQSFRYKMSAEASKLFQTLQESPLPVEQRTKRRVHVGSTNGSGGD *F SGQETNDAKSNGDSRSEKKVLAQGSSSTDEGCETDQGNDPGSASQESKGSNGGGSGNANGGPTSHSSSDLTRLVGTTTSG *F QSHKMRSYASSSSSSGVLAGSAGSYSKSLSQNLSRGSSKSNCSGPYDSLDFALPSGKGSLPSCMGSSSMLATPTPASASP *F AGISSEHSSERSLYGSHNSCIHMPGALPLGLGLPQSSASTPTPNPTPPPNGGGVTFLDKRSPIHFREGRRASDGLVAQGL *F LSSGSLLGTSRVYGSYRYEQAKRHGWLEIQQLQQLQQEAAVGHSHPHAHQHPHQHPHPHPQAYGLEELCQFPNGQFYALP *F GKHHPLLTLPHHHAHPAQHHHGHHSLFHSGHQATPLLLEAAAGGDMYGHGCYAPPPPPPGLYTHHQLGVGMAVPMSPMQK *F PPLQQQLLQHRLLQQKRQLFQKQYALEAQLAGRHHHHQQHSHHHHHHFGHSLGPPPPPAPAPEHHLADELYELALLDRPR *F SGTPRLQHSMTMPGAHAFSQMNLKTSYISQSDQVPGAAPNGAGSGGSAAGVSCSAPPSTAPGTPTVKCKPTTPHGHSLDS *F DYHTSTPVLSLFTPNWQSLVKPLSESPILEISEHLESV *F >CG6114.AA *F MQKENNVTAENCQFVGPYRLEKTLGKGQTGLVKLGVHCVIGKKVAIKIINREKLSESVLMKVEREIAIMKLIDHPHVLGL *F SDVYENKKYLYLILEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIKIADFG *F MASLQPAGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGALPFDDDNLRQLLEKVKRGVFHIPHFVPP *F DCQSLLRGMIEVNPDRRLTLAEINRHPWVTAGGKGELELELPMMEVVQTHVIPTATAVDPDVLNAICSLGCFKEKEKLIQ *F ELLSSSHNTEKVIYFLLLERKRRRPALEDDDEIAQKSRSELDAVDPPRKRLDTCRINGTNAPSYGQISEGSPLTPRRQAF *F NFRSYSSTRNHQRRSPTTVTSSVRSSSYHSPTRCNSPMSSAQQQANAISRPSSPAAGTRHSTYGDRDRSGHHSSVSRTPS *F HSSQKSIEGDVVVVREPRIERRDSLRQERGGGSPRDRGDCGIPPGSPGGNSSGSTSASPSVHHRANSGPTIAIIVNPNGS *F PMMNNSSPGMPGSPCNTPGGQLWKTRLTNIKNSFLGSPRFHRRKMQVSADEVHLTPESSPELTKRSWFGNLITTEKDETF *F TILVKGKPIATVKAHLIHAFLSMAELSHSVVSPTSFRVEYKRNGNGPVMFQRHVKFQVDISAICKQGDIADMLFALTFTL *F LSGNIRRFRRICEHIQSQVCSKRFPGPSSPPTVTSVTQAVSESSSCGSVSSERLSYKRQVIENDMENDSIFSYKSGNGRR *F ASTTNNNNANPVDIPGSPIAVRSNSETAEHERNRELQSERQATTMSGSAIA *F >CG4629.AA *F MRSALRPRGECKLKCLWRRYPNQGVRLQLGAWWQVTIGRRIGLYRFCGDIGRGNFSKVKLAVHQLTRDKVAIKVVDLDRA *F GLDAKALRMLSSEIATLECVHHPNILRLFEVVETLGRVYLVTEWIRGGELYNHITQGGPLREIHAAPLLKQLLLAVKHMH *F SLGYVHRDIKAENVLLLSEDRLKLADFGFSTQLINGTGANQKLDTFCGSPPYAAPELFSDDHYIGAPVDVWALGILLYFM *F VVGNMPFRAPTIPGLKAAILKGDYLLPGQLSLPCIRLIQRILIHIPAQRPTIDDMLNSQFVTCPKLSADLMQWEINQHTK *F PVKRSIFWVRSKSHRLRKSASLRDRYAEVVKKPAISMNTRQQDEMFVQNFLQPIEMGHELLVPVSSQLKEPQSTEQAKRP *F TRRYMFCGSLKKKVTPMETEPEKQLANGGQSIGSAKINPWNVEVAEDCPLFKNYDAETGSCVMLPTNTEDLSQLGALEFE *F ARQILAELGLTSEMLINARQSGPRSDIIGAYRIVVNRLQKQSWLARKHVEMALHSEPKVEKRIERSCCIL *F >Par-1.AA *F MSTAMRTTLQSVPEALPADSVSNGTASNVAAPAAPVSSATNAVPPLAAVSSTTATYATNSISTSSHSVKDQQQQQQQQQH *F DSANANIVSLPPTTTPVANTNTMMPIVTSSNSATSNSTAATPTPASGAAATGGVGSVSQGPATVSASAANTNHSHQHSHQ *F HHHHVANNMTTDGARLSSNNSAVVASSAINHHHHHTPGSGVAPTVNKNVLSTHSAHPSAIKQRTSSAKGSPNMQMRSSAP *F MRWRATEEHIGKYKLIKTIGKGNFAKVKLAKHLPTGKEVAIKIIDKTQLNPGSLQKLFREVRIMKMLDHPNIVKLFQVIE *F TEKTLYLIMEYASGGEVFDYLVLHGRMKEKEARVKFRQIVSAVQYCHQKRIIHRDLKAENLLLDSELNIKIADFGFSNEF *F TPGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGSTLRELRERVLRGKYRIPFYMSTDCENL *F LRKFLVLNPAKRASLETIMGDKWMNMGFEEDELKPYIEPKADLADPKRIEALVAMGYNRSEIEASLSQVRYDDVFATYLL *F LGRKSTDPESDGSRSGSSLSLRNISGNDAGANAGSASVQSPTHRGVHRSISASSTKPSRRASSGVGPTNAAATVAAATGA *F VGAVNPSNNYNAAGSAADRASVGSNFKRQNTIDSATIKENTARLAAQNQRPASATQKMLTTADTTLNSPAKPRTATKYDP *F TNGNRTVSGTSGIIPRRSTTLYEKTSSTEKTNVIPAETNLFDRLRMASAVKSSRHFPRNVPSRSTFHSGQTRARNNTALE *F YSGTSGASGDSSHPGRMSFFSKLSSRFSKRPTIADEAAKPRVLRFTWSMKTTSPLMPDQIMQKIREVLDQNNCDYEQRER *F FVLWCVHGDPNTDSLVQWEIEVCKLPRLSLNGVRFKRISGTSIGFKNIASRIAFDLKL *F >KP78a.AA *F MSVDNSLGILDLSSGKESEKTAMTAANKENDPDSHFTGTIVTTGATAQPATPTTSNATALPATPTVAAGQDLGDGACSSK *F NTDSKFQSYVNGNGNGVYKIIKTLGKGNFAKVKLAIHVPTGREVAIKVIDKTQLNTSARQKLYREVKIMKLLNHPNIVRL *F FQVIESERTLYLVMEYASRGELFDHLVKNGRMRERDARVIFRQLVSAIQYCHSKFVVHRDLKAENLLLDQHMNIKIADFG *F FGNTFDPNAQLETFCGSPPYAAPELFMGRKYAGPEVDAWSLGVVLYTLVSGSLPFDGGTLKELRERVLRGKYRVPYYISM *F DCENLMRKFLVLNPAKRTSLSAVMSDKWINLGHDESDRLRPFREKPMELQDAARFDLLMSMGHKRRDVEQSVKGQLFDDI *F YCTYMLLGVAKPRSSNRSTKPEAIPTVDLTTPAVSSPLPNITTPTVTIAHVTLALDKNPPIHSSSASGRPIAPRLANAPN *F TPTSTPPTGPPAKPTRRTPARTPARKATNHTSGQGRPEPSSLPHTPQSKRASANMDVKPTLLSAQRQLAQTQKLANTPPR *F FQYPVAQTPNQAQGSSRRPTTLYEKAEPAVTPLLVPKSPAMGARLLERIPGGGAVEKTSDKPFTRQNVARATFHFGQARS *F GKRGGGSGNGAGGEEDSYQTPPLSADDTKPASRVGFFSKLTARFGRRVIHLNEKDVTEQRKNLTK *F >KP78b.AA *F MTAANTNKTTDKENDPGPNTSISTTATPPSAAAQNVGGCFGSSGGRSSPKFQSYVNGNGYGVYKIIKTLGKGNFAKVKLA *F IHLPTGREVAIKLIDKTALNTIARQKLYREVNIMKKLNHPNIVRLLQVIESERTLYLVMEYVSGGELFNYLVKNGRMRER *F DARVLFRQLVSAIEYCHSKSIVHRDLKAENLLLDQQMKLKIADFGFSTTFEPKAPLETFCGSPPYAAPELFRGKKYSGPE *F VDSWSLGVVLYTLVSGSLPFDGTNLKELRDRVLRGKYRVPYYVSIECESLIRKFLVLNPTQRTSLSAVMADRWINMGYEQ *F GNGLRPFQEKPMDLHDVNRLSLLSNMGHKPRDVEQSLKNQKFDDIYCTYMLLDVAKPRSTACSEKSGSSFRETPTAMPGS *F SRIPVPIAAPNVTISQVTFALDKSTPNRPAATSIRPMAPRLANALTPLPLTPPPKKYICCSASKAANPRRSEPSSIPQSA *F MPKKGVGSPVDVKTTLLSAQRKLAVNQKLTSASHQIRSPITQSPSQASECTRTPPTHFEMLDSTSTPLKVLKLVASNSQT *F PPSTENTNRPTRVGFFSKLSARFVRRSLHKGEKDISEQGRNLTK *F >CG10177.AA *F MSEQSTSLGSRRVGPPLHKKALRVCFLRNGDRHFKGVNLVISRAHFKDFPALLQGVTESLKRHVLLRSAIAHFRRTDGSH *F LTSLSCFRETDIVICCCKNEEIICVKYSINKDFQRMVDSCKRWGQHHLDSGTLESMKSHDLPEAIQLYIETIEPVEHNTR *F TLIYRGQTRANRTKCTVKMVNKQTQSNDRGDTYMEAEVLRQLQSHPNIIELMYTVEDERYMYTVLEHLDCNMQKVIQKRG *F ILSEADARSVMRCTVSALAHMHQLQVIHRDIKPENLLVCSSSGKWNFKMVKVANFDLATYYRGSKLYVRCGTPCYMAPEM *F IAMSGYDYQVDSWSLGVTLFYMLCGKMPFASACKNSKEIYAAIMSGGPTYPKDMESVMSPEATQLIDGLLVSDPSYRVPI *F AELDKFQFLAL *F >Caki.AA *F MTEDEIVFDDVYELCEVIGKGPFSIVRRCIHRESNQQFAVKIVDVAKFTASPGLSTADLKREATICHMLKHPHIVELLET *F YSSEGMLYMVFEFMEGSDLCFEVVRRAVAGFVYSEAVACHYMRQILEALRYCHENEILHRDVRPACALLATVDNSAPVKL *F GGFGSAIQLPGTRETIETHGRVGCPHYMAPEVVTRRLYGKGCDVWGAGVMLHVLLSGRLPFLGSGVRLQQSVARGRLSFE *F APEWKSISANAKDLVMKMLAANPHHRLSITEVLDHPWIRDRDKLQRTHLADTVEELKRYNARRKLKGAVQAIAGGTNMDP *F LYATDADMPITGATDEWADEEAGIEAVQRILDCLDDIYSLQDAHVDADVLRDMLRDNRLHQFLQLFDRIAATVVTSNGRA *F PAAEAVGRCRDVLEQLSSTSGGNSLGGKYAKEELMRLLAAPHMQALLHSHDVVARDVYGEEAARHPAPDGALPQWRELDN *F VEGGELQHVTRVRLVHFQKNTDEPMGITLKMTEDGRCIVARLMHGGMIHRQATLHVGDEIREINGQPVQHQSVGQLQRML *F REARGSVTFKIVPSYRSAPPPCEIFVRAQFDYNPLDDELIPCAQAGISFQVGDILQIISKDDHHWWQARLGTVGGSAGLI *F PSPELQEWRIACQTVDKTKQEQVNCSIFGRKKKQCRDKYLAKHNAIFDTLDVVTYEEVVKVPVGDPNFQRKTLVLLGAHG *F VGRRHIKNTLISKYPDKYVYPIPHTTRPAKPEEENGRSYYFVSHDEMMADIGANEYLEYGTHEDAMYGTKLDTIRRIHTE *F GKMAILDVEPQALKILRTAEFTPYVVFIAAPSLQNIADYDGSLERLAKESEMLRQLYGHFFDLTIVNNDISETIATLETA *F IDRVHTTPQWVPVSWLY *F >otk.AA *F MTARMISICGLVMALMMASVLASSSRFQRVPQSQSVVENESVKFECESTDSYSELHYDWLHNGHRIAYDKRVHQIGSNLH *F IEAVRRTEDVGNYVCIATNLASGAREASPPAKLSVIYLESASVQLLGSNRNELLLKCHVEGASGDLEPLEIEWYRNSEKL *F STWKNVQLDQHRLIIRQPGSEDDGLYRCTASNAAGRVMSKQGYVYQSSVKCLPRLPRRKNEKMMESWDKQTFLCRGKRGG *F AAGLEALPAAPEDLRIVQGPIGQSIIKEGEPTALTCLYELPDELKNQRIQLRWRKDGKLLRQVELGGSAPIPGHSFDSGK *F DALLREDARLVLHKQNGTLSFASIIASDAGQYQCQLQLEAHAPINSSPGILEVIEQLKFVPQPTSKNLELDAVVAKVHCK *F AQGTPTPQVQWVRDGENTTLPDHVEVDANGTLIFRNVNSEHRGNYTCLATNSQGQINATVAINVVVTPKFSVPPVGPIET *F SEQGTVVMHCQAIGDPKPTIQWDKDLKYLSENNTDRERFRFLENGTLEIRNVQVEDEGSYGCTIGNSAGLKREDVQLVVK *F TTGDGFAPEESGGDGFLVTRAVLITMTVALAYIVLVVGLMLWCRYRRQARKARLNDLSTKEAGGDQPDVAGNGKGSEQEP *F CLSKQHNGHSKSRSKSSGDAQKSDDTACSQQSRASKKSAHIYEQLALPRSGLSELIQIGRGEFGDVFVGKLKATLVTSPS *F DKDADTEKQHSNSENGSGGSGSGSTTLSTLNEKRRSKTSMDDIEEIKEEEQDQHNQSGLEQLVLVKALNKVKDEQACQEF *F RRQLDLLRAISHKGVVRLFGLCREKDPHYMVLEYTDWGDLKQFLLATAGKVNTATAGSSSPPPLTTSQVLAVAYQIARGM *F DAIYRARFTHRDLATRNCVISSEFIVKVSYPALCKDKYSREYHKHRNTLLPIRWLAPECIQEDEYTTKSDIFAYGVVVWE *F LFNQATKLPHEELTNEQVVQRSQAGSLEWSVAEATPDSLREILLSCWVSNPKERPSFSQLGAALSKAMQSAEK *F >CG8655.AA *F MADRVMCQELATPGSHYRRPKTGVPSTNVVYANGGGSWRAAPRQTQRLPRQTVQQYATGTPATGSAGRHSGRAFATAGAT *F EEQTVEFNGAAVMGLPATPRTTKQARNKSEEAINDLLTRIPDIGKLFDVHSRIGNGTFSTVLLGTLRRESHLPDSLRRKF *F AIKHHIPTSHPDRIMKELQCMTKMGGKENVVGIHCCMRYDASAAFVMPFMAHDRFQDFYTRMDVPEIRQYMRNLLVALRH *F VHKFDVIHRDVKPSNFLYNRRRREFLLVDFGLAQHVNPPAARSSGSAAAIAAANNKNNNNNNNNNSKRPRERESKGDVQQ *F IALDAGLGGAVKRMRLHEESNKMPLKPVNDIAPSDAPEQSVDGSNHVQPQLVQQEQQQLQPQQQQQQQQQQQQSQQQQQP *F QQQSQQQHPQRQPQLAQMDQTASTPSGSKYNTNRNVSAAAANNAKCVCFANPSVCLNCLMKKEVHASRAGTPGYRPPEVL *F LKYPDQTTAVDVWAAGVIFLSIMSTVYPFFKAPNDFIALAEIVTIFGDQAIRKTALALDRMITLSQRSRPLNLRKLCLRF *F RYRSVFSDAKLLKSYESVDGSCEVCRNCDQYFFNCLCEDSDYLTEPLDAYECFPPSAYDLLDRLLEINPHKRITAEEALK *F HPFFTAAEEAEQTEQDQLANGTPRKMRRQRYQSHRTVAASQEQVKQQVALDLQQAAINKL *F >CG5790.AA *F MDKKEVQHHRSQQAMTKSLYAIGMGLLGKSATNMDCANTNLESAGKSSSTNYNRNRFYNERFVPMASGTMPSVSGTNHHS *F RHQQLLLQQAPKCTADLNEKLVKINRQNANKELSTIQAKDMQSVDKNEEALKELQESIPEINKIFDVHCRIGSGTFSTVL *F LGTLQRERGLVETQRRRFAIKHHNPTNHPERILRELECMYRIGGVENVIGINCCIRYNDNVAFIMPYMTHDRFHDIYRSL *F NFPEIRDYLRNLLIALRHVHKFNVIHRDVKPSNILYNRRTGKFLLCDFGLAQRIADDGSVVQSSDLSSREVFSILRDLEN *F GRSVTLTDGNSAQAEAEDYMARRRMRALGGGGSVERAVTGPPSIQKLREQAGGHLTKKDVANQRADTMRLLNRLRLVSPN *F ADPNNYVVSTNTSKKEMHASRAGTPGYRPPEVLLRYPKQSTAVDVWAAGVIMLSLLSGLHPFFKAPHDCGALAEIINLFG *F DMPVRKTAFLLDRLILLAQKVNTLDLRRVCMRFRHADFFLAPEIQRKYQRPDGTTEMCRSCEQPTFNCLCSNSGHNLERY *F DGLDIFPAVAYDLLSRLLEVNPQKRITAEEALKHPFFSDQHRITPGIPLHQQQLIMHQQQHIMQHQQHLQRSRETLPSSA *F ARTLKAFVCYPMEITSTASQAAGNI *F >cdc2.AA *F MEDFEKIEKIGEGTYGVVYKGRNRLTGQIVAMKKIRLESDDEGVPSTAIREISLLKELKHENIVCLEDVLMEENRIYLIF *F EFLSMDLKKYMDSLPVDKHMESELVRSYLYQITSAILFCHRRRVLHRDLKPQNLLIDKSGLIKVADFGLGRSFGIPVRIY *F THEIVTLWYRAPEVLLGSPRYSCPVDIWSIGCIFAEMATRKPLFQGDSEIDQLFRMFRILKTPTEDIWPGVTSLPDYKNT *F FPCWSTNQLTNQLKNLDANGIDLIQKMLIYDPVHRISAKDILEHPYFNGFQSGLVRN *F >Cdk5.AA *F MQKYDKMEKIGEGTYGTVFKGRNRDTMEIVALKRVRLDEDDEGVPSSALREICLLKELKHKNIVRLIDVLHSDKKLTLVF *F EHCDQDLKKYFDSLNGEIDMAVCRSFMLQLLRGLAFCHSHNVLHRDLKPQNLLINKNGELKLADFGLARAFGIPVKCYSA *F EVVTLWYRPPDVLFGAKLYTTSIDMWSAGCILAELADAGRPLFPGSDVLDQLMKIFRVLGTPNEDSWPGVSHLSDYVALP *F SFPAITSWSQLVPRLNSKGRDLLQKLLICRPNQRISAEAAMQHPYFTDSSSSGH *F >Pitslre.AA *F MVNSSGSEDGQLRSPNDVHYHSRGEEDEHEGDADALYIQPPQASRESGSGPRREKKKHSRERRRHKERDDVGGAALALER *F DHRYDYRSREEHYHHHQRERSSNAAAAYAKHHLGHAYHYPQPPQQQQQPLPPAPSYAAHHYHHHQHLSGARAAPREYHSY *F PSGYHSGSRHGDYPMEEPTRRSSKYAESKDAESLEQDLRSRLLKKRHNYVKDYETEENYEHRVERSDRREGGRKERERTV *F RSTHKQNRHDRVIELLDSPEQEHHHQHQHKSHRSKWREEVEVIRRKVPEDLELLARREKLLAAERESRQRKQTAREELEA *F RRELLRERNEHSDALSPTTVAASVTAGLNIHVKRKSKPDNYEKEIKLKKRREDDIEVIRDDDDEESEESDSNEEVPEQDS *F EGSATESGSEDSYASKKKSKIKSKSQLEDDDEDLPLPDSPLSVGELYKSPKQRQRSRSVSSKSSSQSSRSSRSRSRSRSQ *F SSLEDEVDRQDVGADASPSSSTRSEERGMTQEQPEEKPEEKLKEKQKSLEEQIPCDDKGIPLPNYYPGVQGCRSVEEFQC *F LNRIEEGTYGVVYRAKDKRTNEIVALKRLKMEKEKEGFPITSLREINTLLKGQHPNIVTVREIVVGSNMDKIFIVMDYVE *F HDLKSLMETMKNRKQSFFPGEVKCLTQQLLRAVAHLHDNWILHRDLKTSNLLLSHKGILKVGDFGLAREYGSPIKKYTSL *F VVTLWYRAPELLLCSPVYSTPIDVWSVGCIFAEFLQMLPLFPGKSEIDELNRIFKELGTPNEKIWPGYTELPAVKNMLSQ *F NSQFTEYPVSQLRKHFQEKTSEMGLSLLQGLLTYDPKQRLSADAALKHGFFKELPLPIDPSMFPTWPAKSELGARKAQAS *F SPKPPSGGSQFKQLGRDEPIIVGPGNKLSSGIITGNKKSHGAGGSSASTGFVLNAGITQRQLAMGPGFSLKF *F >cdc2rk.AA *F MSSLKSNDVDTNPAPDPQAPITRKGFLMSLNTGTPMPIPEQNLFGRCRPVSEFEKLNRVGEGSYGIVYRARDTRSNEIVA *F LKKVRMDQEKDGLPISGLREIMILKQCHHENIVRLREVVVGKSLDSIFLVMDFCEQDLASVLDNMSQPFTESEVKCITLQ *F VLKALKYLHSRFMIHRDLKVSNLLMTDKGCIKVADFGLARMFSNPPKPMTPQMVTLWYRAPELLLGCRTHTTAVDMWAFG *F CILGELLLGKPLLPGNSEIAQLDMIIDLLGAPSESIWPGFADLPAVQNFTLSQQPYNNLTPKFHMIGQSGRNLLNILFIY *F NPKTRATAEECLKSKYFVDPPQACDPGMMPTFPQHRNNAAPAPAVQPPADIPISDLLNVFIKRQRME *F >cdc2c.AA *F MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNL *F YMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMR *F AYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFK *F TKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV *F >Cdk4.AA *F MSYVRQLKRQKMSQAKKFGDGDPFNYQELNIIGEGAYGTVYRARDVITGNIVALKKVRISLNENGVPMSTLREISLLKQL *F NASNHANIVKLYEVCQFLERDGQLLILLVFEHVEQDLSDLIDRLPKSGMSPPTIQRLSRELLTGVDFLHSHRIIHRDLKP *F QNLLVSSQGHLKIADFGLAKTYGSEMKLTSVVVTLWYRAPEVLLAQPYNSTVDIWSAACIIFEMFNRRALFPGTSEKNQL *F DRIFELTGRPTEQQWPQTISVALEHFPQRHPKRPKDFCPHLCKYADDLLNKMLSYDLHLRPSALACLEHDYFQQEPL *F >CG6800.AA *F MEDYAPSRYKMLEKIGEGVHGCVFKAIDLQRNKEVAIKKVALKNKFGNIALNTLREIKTLQLCKSEYILDIIDIYPDLTG *F LSLVLEYQPDTLYNRLKSEVNPLSRQQVRKFAHQMFKGIAYLHEAGLMHRDIKPANLLISDTDMLKIADFGLARLYFPED *F ESRLYSPQVSTRWYRAPEILFGSQKYGTGVDMWAAGCVVAEMLRGVPLFAGTTDIEQLAIIIRTLGSPRLNQWPELTSLP *F DYSKIRFPNSVGIHWDNLFPSCTHAVEINLVSNLVVYNPKNRLKASEVGSASSLTPVRYYNW *F >Eip63E.AA *F MAELISQPTKINNNNFGAGVTMREKKGGALQKLKKRLSHSFGRLTISREDGDESTHHHHHHHHHRGGHGGHHNHGNKVPY *F NGYNSEEYLDRLEPNGNIPADKTNCRYGDWRSTDSTDHHDRVQRQLSVSSDSKLLDEDIREEMKYHSHVVMRPKKPPRPK *F SEVFLNKQETHPRRKRFSAFGGDSPFGKQEAYVKLEPLGEGSYATVYKGFSKLTYQRVALKEIRLQEEEGAPFTAIREAS *F LLKELKHSNIVTLHDIVHTRETLTFVFEYVNTDLSQYMEKHPGGLDHRNVRLFLFQLLRGLSYCHKRRVLHRDVKPQNLL *F ISDCGELKLADFGLARAKSVPSHTYSHEVVTLWYRPPDVLLGSTEYSTSLDMWGVGCIFVEMVTGMPTFPGIRDTYDQLD *F KIFKLLGTPTEDTWPGVTHFPGYKPHKLGFYRPRKLGHNFPRLYDIIEGETIANGFLQLNPEQRLGADDALQHPYFAQLP *F KKLYELPDGENLNFHRGRRAALHGAKSTE *F >Cdk8.AA *F MQNVRAYPYQPATSNMNAYCLHNMQYQQQRLPFHQQQQQHHQQLQQQQSQFQPLRSHQLIGGIFIYKQLWNLQTFVKMDY *F DFKMKTQIERTKVEDLFNYEGCKVGRGTYGHVYKAKWKETSDGKEYALKQIDGTGLSMSACREIALLRELKHQNVITLIR *F VFLSHNDRKVFLLIDYAEHDLWHIIKFHRAAKATKKQVVVPRGMVKSLLYQILDGIHYLHSNWVLHRDLKPANILVMGDG *F NERGRVKIADMGFARLFNAPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKT *F SNPYHHDQLDRIFNVMGFPQDKDWEDIKKMPEHHTLTKDFKRSTYSTCSLAKYMERHKIKPDSKAFHLLQKLLLMDPNKR *F ITSEQAMQDQYFQEEPQPTQDVFAGCPIPYPKREFLTDDDQEDKSDNKRQQQQQQQQQQQQQQQQQQQQQQQQQQQQMNA *F EPNAKRVRLSGAGNQQDFHHQQQQQQQQQQQQQQQQQQMMFNQQQNFQRFN *F >Cdk9.AA *F MAHMSHMLQQPSGSTPSNVGSSSSRTMSLMEKQKYIEDYDFPYCDESNKYEKVAKIGQGTFGEVFKAREKKGNKKFVAMK *F KVLMDNEKEGFPITALREIRILQLLKHENVVNLIEICRTKATATNGYRSTFYLVFDFCEHDLAGLLSNMNVKFSLGEIKK *F VMQQLLNGLYYIHSNKILHRDMKAANVLITKHGILKLADFGLARAFSIPKNESKNRYTNRVVTLWYRPPELLLGDRNYGP *F PVDMWGAGCIMAEMWTRSPIMQGNTEQQQLTFISQLCGSFTPDVWPGVEELELYKSIELPKNQKRRVKERLRPYVKDQTG *F CDLLDKLLTLDPKKRIDADTALNHDFFWTDPMPSDLSKMLSQHLQSMFEYLAQPRRSNQMRNYHQQLTTMNQKPQDNSMI *F DRVW *F >Cdk7.AA *F MLPNANDKTERYAKLSFLGEGQFATVYKARDTVTNQIVAVKKIKKGSREDARDGINRTALREIKILQELQHENIIGLVDV *F FGQLSNVSLVFDFMDTDLEVIIKDNKIILTQANIKAYAIMTLKGLEYLHLNWILHRDLKPNNLLVNSDGILKIGDFGLAK *F SFGSPNRIYTHHVVTRWYRSPELLFGARQYGTGVDMWAVGCILAELMLRVPFMPGDSDLDQLTRIFSTLGTPTEAEWPHL *F SKLHDYLQFRNFPGTPLDNIFTAAGNDLIHLMQRLFAMNPLRRVSCREALSMPYFANKPAPTVGPKLPMPSAILAAKEGA *F NPQTGDTKPALKRKLVETTVRGNGLAQKKRLQF *F >CG7597.AA *F MHASSAAATALVEYSDVSSEDFSDQEAGDLDADAGKGAGNIKKPKPAPDNQFSKGRLDAKPDKEGYDNYRSRRAEDSSDP *F VAAGSRQTSSSEATNPREEPSQASNTSKDELWGREIYMSSDSIDTDELEAEMKRQKRKKQKKEKHKHKSKKKSKKRKKKR *F AKSYSSIDSMSDNDINALLDRRYTPPTAPSKSNERTVSAAPSSFTPHNLKESSSPATPPPVRRPNTNSNYYGESSLETAN *F SALGSNLQVTVTNKQSISNRLRSPPPSSRSSGNGPRFGNSPRTPPPSHYSSSGGGGVGSGSVVRDSRSSRYVNSPHKEDV *F SAHHRSSHDHGYQGRYSGAGSSSHDTRKVKRLSPELDRYNHQPSTPPHKRRKFSDGREVGLGNFEHSRHHSGKYERYSRD *F RYSRRSSRSPSVQHSRSRQSPSGGLSSGSNAFRHGGSHKHKYGTTVSSTPSHTTRTSKRASGTGTSGDRYSRSPRTSSRY *F MESSPPSPVGASGSHHYHHRRSPRMRQRTRGDSRRRSPSSASSESSASRSRSPTSRDLKHKREEYIKKISETSLFAELVK *F DRHKRQKALKEIIERQEENSNSNSNGALTINDNSSSVDGNTPNAADGRSAPGSGTPAAASTTSNGLQALGSKPDLDLNNI *F PMPNKQNDSVVSNPASNADVPDSVAQLKQPLLVPPFSASKNNIKPKSLTSLPLPPGMNVLDLAGARSPSPGQKKESDEKN *F VTSSGSANKSVLNLPMPPVIPGSEELSGDDDVIDSPEDFDAPAVGTVHGHGGGPGTTRQRPVILNRRDSRNNVRDWGERC *F VDVFEMIAQIGEGTYGQVYKARDHHTNDMVALKKVRLEHEKEGFPITAVREIKILRQLNHRNIVNLHEIVTDKQDAVEFR *F KDKGSFYLVFEYMDHDLMGLLESGMVDFNEENNASIMKQLLDGLNYCHKKNFLHRDIKCSNILMNNRGKVKLADFGLARL *F YNADDRERPYTNKVITLWYRPPELLLGEERYGPSIDVWSCGCILGELFVKRPLFQANAEMAQLETISKICGSPVPAVWPN *F VIKLPLFHTLKQKKTHRRRLREDFEFMPAPALDLLDKMLDLDPDKRITAEDALRSPWLRKINPDEMPTPQLPTWQDCHEL *F WSKKRRRQMREQQESLPPTVIASTKYQQHGATMVGDA *F >CG7236.AA *F MDRYEKLSRLGEGSYGVVYKCRDRETGALVAVKRFVESEDDPAIRKIALREIRLLKNLKHPNLVSLLEVFRRKRRLHLVF *F EFCELTVLHELERHPQGCPEHLTKQICYQTLLGVAYCHKQGCLHRDIKPENILLTAQGQVKLCDFGFARMLSPGENYTDY *F VATRWYRAPELLVGDTQYGTPVDVWAIGCLFAELVRGEALWPGRSDVDQLYLIRKTLGDLLPRHIQIFGQNEYFKGITLP *F VPPTLEPLEDKMPAKSQQNPLTIDFLKKCLDKDPTKRWSCEKLTKHSYFDDYIAKQRELEHVNSLEAANLRQQQLASQQF *F MLATAAQQLQTGPAQAAAIAAARDKSKTSNTSLPLLPSTQHHHHPHQDYVKLQPLNKNANLLHRTEHHLPTI *F >CG9962.AA *F MNDYELETMLRINSIMVIRKLGSGSFGDIYEAKHMGSGLHVALKVERKNAGQSHLSIESTVYNLLRHGMGIPMTYQFFSN *F RRHDVLVMELLGPSLETLFTMCNRRFSMKTVLMLADQMVDRLEYLHLHHYVHRDIKPENFLMGVGLTRHRLHLIDFGLSK *F RYWDMKENRHVPQRRGTKWAGTARYASVNALCCKVQSRRDDLESVGYVLIYLLRGSLPWQGLLPNSKLQKAEMILEMKLS *F TLPNSLCAGYPNEFYNYIIYTRQLGFEEEPDYRMIRCTFLSLLFNLKFTNDLIYDWDHAEKNSGKSGSEEDRVVVKKVV *F >gish.AA *F MYSTRQSVSTTTGVLMVGPNFRVGKKIGCGNFGELRLGKNLYNNEHVAIKMEPMKSKAPQLHLEYRFYKLLGSHAEGVPE *F VYYFGPCGKYNALVMELLGPSLEDLFDICGRRFTLKSVLLIAIQLLHRIEYVHSRHLIYRDVKPENFLIGRTSTKREKII *F HIIDFGLAKEYIDLDTNRHIPYREHKSLTGTARYMSINTHMGREQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERY *F QKIGDTKRATPIEVLCDGHPEEFATYLRYVRRLDFFETPDYDFLRRLFQDLFDRKGYTDEGEFDWTGKTMSTPVGSLQTG *F HEVIISPNKDRHNVTAKTNAKGGVAAWPDVPKPGATLGNLTPADRHGSVQVVSSTNGELNPDDPTAGHSNTPITQQPEVE *F VVDETKCCCFFKRKKKKSTRQK *F >CG7094.AA *F MAKQEATNGKVRNASLHLEKLLIGGKYRLVKPIGSGSFGDIYLGLSITDGSEVAIKVEKNDAKYPQLIYEAKVYEQLARC *F PGFPTLLHYGCEKNYNAMVMDLLGPSLEELFNLCKRRFSLKTVLMLTDQLLMRIECVHERGFIHRDIKPDNFLMGLDRHC *F NKLYLIDFGLSKRYKDIESEIHIPYRTDRNLTGTVRYASINAQIGVEQSRRDDMESMSYCLMYFNLGKLPWQGITAANKK *F QKYEKILEKKTSVTIAQLCKGFPSEFCLLMTYVRNLGFKEPPDHTYLRQIFRILFRSLNHHYDYIYDWTALQQQKDQICR *F SREQILESEREEVRKRDGERGCEPQRDKERHKDLELDRLHKTSTQQAKCSNGHLTNRYDRIGDGGISKRK *F >CkIalpha.AA *F MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIR *F HHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLID *F FGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKIS *F EKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLE *F QLDKDKEKQNGKPLIAD *F >CG2577.AA *F MERLRSSQNREVRIGNYKVVRKIGCGSFGDIYLGIYIHSGERVAIKVESSKVRHPQLNYERRIYRALRPAHGLPRIRYFH *F KEEHYQAMVMDLLGPSLERLFQFCERAFTIKTVLLLAEQMLRRVEYVHNRGFLHRDIKPDNFLMGLGTMSKQVYLIDFGL *F SKKYLDITTGVHIPYREERSLTGTARYASIGAHAGVESARRDDMVAVGYVLMYFNRGSLPWQDLKASTKQQKYERIHEKK *F ISVSIEVLCEGFPCEFTMYLNYCRGMGFYDKPNYDFICRMFRMLRNGLNLRPGLIYDWDMLMMKFHNTQKNPGIGMRVFP *F PKQKEDDGISGEPIIKDEKCNFWP *F >CG12147.AA *F MAEREKGRAKDDRRWQGRSDVHQSKPQIRKERRHPPQPNHPHEKDQRQDRRFSEEKQSLPEIIVAGKYRLLKRIGNGSFG *F ELFQAEGLKYHEKVAIKLESSTVKHPLLPREARIYGILQGGLGIPHVKHYATEGAYNVMVMDLLGPTLEDLLNLCSRSFS *F MKTTLMLADQILARVELLHRRCFIHRDIKPDNFLMGLNRHQTQVYMIDFGLAKKFYSLRTQKHIGYTENRDLVGTARYAS *F VRAHYAEQSRRDDLESVGYLLLYFQRGRLPWQGIRAQSQAQKYEKIAEYKANIPLQQLCSGLPVEFFMYLKYCRKLHFAE *F KPDYVYLQQLFKVLFRNQYKVCDFLFDWVVLKRESPEQQSQQKGRERDRGGKRNVVVQKERHRNKDRDSDTQRCRIKNGG *F DLLEIEENESTTGEDPNEDKPQKKHGKACSCSYHLQKKRLQDRDRRAPLMTERRILSGEQEQELELELRGSPQMPCL *F >dco.AA *F MELRVGNKYRLGRKIGSGSFGDIYLGTTINTGEEVAIKLECIRTKHPQLHIESKFYKTMQGGIGIPRIIWCGSEGDYNVM *F VMELLGPSLEDLFNFCSRRFSLKTVLLLADQMISRIDYIHSRDFIHRDIKPDNFLMGLGKKGNLVYIIDFGLAKKFRDAR *F SLKHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGALPWQGLKAANKRQKYERISEKKLSTSIVVL *F CKGFPSEFVNYLNFCRQMHFDQRPDYCHLRKLFRNLFHRLGFTYDYVFDWNLLKFGGPRNPQAIQQAQDGADGQAGHDAV *F AAAAAVAAAAAASSHQQQQHKVNAALGGGGGSAAQQQLQGGQTLAMLGGNGGGNGSQLIGGNGLNMDDSMAATNSSRPPY *F DTPERRPSIRMRQGGGGGGGGVGVGGMPSGGGGGGVGNAK *F >CkIIalpha.AA *F MTLPSAARVYTDVNAHKPDEYWDYENYVVDWGNQDDYQLVRKLGRGKYSEVFEAINITTTEKCVVKILKPVKKKKIKREI *F KILENLRGGTNIITLLAVVKDPVSRTPALIFEHVNNTDFKQLYQTLTDYEIRYYLFELLKALDYCHSMGIMHRDVKPHNV *F MIDHENRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQL *F VRIAKVLGTEELYAYLDKYNIDLDPRFHDILQRHSRKRWERFVHSDNQHLVSPEALDFLDKLLRYDHVDRLTAREAMAHP *F YFLPIVNGQMNPNNQQ *F >Doa.AA *F MCVRFQMPRTRRLHHSRDRSSAGTRDKRRRHDTADHSPPLAEAPSPPRITNTHHTRSAAKRRRHELDAKKAQISKEPTFD *F DSISTRRRKERSKRSHRKSPAASRRQHKYRYRDETSHSSSRRRHRDRAKDERDSGRNNRQSQAKTAKPVIQDDADGHLIY *F HTGDILHHRYKIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYREAAKLEINALEKIAQKDPHCDHLCVKMIDWFD *F YHGHMCIVFEMLGLSVFDFLRENNYEPYPLDQVRHMAYQLCYSVKFLHDNRLTHTDLKPENILFVDSDYTSHYNHKINRE *F VRRVKNTDVRLIDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCILFELYLGITLFQTHDNREHLAMME *F RILGQIPYRMARKTKTKYFYHGKLDWDEKSSAGRYVRDHCKPLFLCQLSDSEDHCELFSLIKKMLEYEPSSRITLGEALH *F HPFFDRLPPHHRVGEVSNKQPLSSGSSSRERSHSLSR *F >CG17309.AA *F MSESIAIGFLQYIITINYDDEEAQARKTSHEKSQKPKPKPEENHKEKEAISRKASGEGDLITVEIKQGLLRKQTATTTTG *F QAMNSHATATQNGQTTVVGHHRGFSQPSVTVAAAAATGSTAGDPWFLQTTGHQMPPTAPLRHNKRPAPQPNAVLGAGSSG *F TAPGSAVGAVLLHQQQLSQSQPHIVPPSIPPHHHHHRENNVHPIQAGAAAALHLSSHALNSHNLISSASNHSPKSPSQTQ *F QQQQTTNQQQQQQQQQASHSILSTFAPAPTVAAVQPAGSQSVAGTAAAVAAVAQQQQQQHLQLLANCPPGGAGLMSSSLN *F AAQMGMHGAERALPPKSLALSGSQHGSNMLLHTAPQPPQASQPAAVVGVSGSGSAGMSTSLHSFDINGVTAGSGVNASSA *F WSSGSTTAMNHASLSPTALAPQQGRSRCEVKLNAMPWFHGSITRDEAEHLLQPREDGLFLVRESTNFPGDYTLCVCFQSK *F VEHYRVKYLENKLTIDDEEYFENLGQLVAHYEADADGLCTQLIKCLPKLGKQEFCINSKDFVDKGWVIPEAELQLRESIG *F KGEFGDVMLGILRNEKVAVKMLKDEGAVQKFLAEASVMTTLEHDNLVKFIGLVFTSKHLYLVTEYMSKGSLVDYLRSRGR *F QHITKKDQIIFAYDTASGMEYLEAKKVVHRDLAARNVLISEDCVAKVSDFGLAREECYNLDVGKLPIKWTAPEALKNGRF *F SNKSDMWSFGILLWEIYSFGRVPYPRIPLADVVKHVEVGYKMEAPEGCPPEIYEMMRQAWDLNPAKRPTFAELKVKLQLL *F NNATAHGQSQSLPEMGQIQS *F >CG1760.AA *F MIAAGSRTVGKFAAVRRAIHKNTGSHFAAKFLKRRRRAQSSDKEIKHEIAVLMLCEGEDNIVNLNAVHETRSDTALLLEL *F ATGGELQTILDNEECLTEAQARHCMREVLKALKFLHDRSIAHLDLKPQNILLAGERIEDGLKLCDFGISRVVCEGINVRE *F MAGTPDYVAPEVLQYEPLSLLTDIWSVGVLTYVLLSGFSPFGGDTKQETFLNISQCALTFPDNLFGGVSPVAIDFIRRAL *F RIKPNDRMNATGCLDHIWLKDDCSLDRQIYLQPQSDAEEEEEEDVDDDVEDEEEEEQVEEEEEETQNVEEPTTEAPQQQQ *F QPVQQHQLAKSGQIHSKPTHNGHHRANSGGSISKIPIATSKLQSTPSSETTTAIHTLTSNSNGHVHKPTQIVTPTRRASD *F SDKENTYTATFVKKPVATIQLGSSNGIEDATVVATLTLFPDAPTTPKVIRKTPTGESNGSATSVKALVKKFQLEDSSGSA *F VARRSGGAVTSSSGLHSPTTTSVRLNSIRRASEPLTTVYKKQTSQNGCSSTSNPSSSPGSSPTTSGSTATLLIHSQRLGN *F STAPASHCVAVPATATATSSASSSNSSSGKSTSAAHHLHHHHMHHHHHHHHHHVVIAAKNAAAVAATNNLSLDQGIIC *F >CG17528.AA *F MELEKVKINSLHCNDAVLSSLQASPSATSPQSVPSKANVVTEASIIEKTNQPHNVQEDNNYNRDCDSPESSSSEQDKELD *F DLRNLHSSSLTNSVVVGKSTGSLNGVYSITSVTSETKTLESVVTTNSASGSACLSNTPTADHIKKRIPSSRTPTRKALRI *F KFYRNGDRFYPGITIPVSNERYRSFERLFEDLTRLLEENVKIPGAVRTIYNLCGKKITSLDELEDGQSYVCSCNNENFKK *F VEYNTGSQPLSNLPLSNSSSPFPACGGGTGNGSPLIASRLSDRVTVVHPRIVTLIRSGTKPRRIMRLLLNKRNSPSFDHV *F LTAITQVVRLDTGYVRKVFTLSGIPVVRLSDFFGSDDVFFAYGTERINTAEDFKLEAEEQRAINVIRKTMRTTGTTCKGP *F KPKMPIKSKKVYPPLVDSEPFKAETTPEDDRHAALLTSTGMEINELPSNIRNTYSLGRIIGDGNFAIVFKIKHRQTGHSY *F ALKIIDKNKCKGKEHYIDAEVRVMKKLNHPHIISLILSVDQNTNMYLVLEYVSGGDLFDAITQVTRFSENQSRIMIRHLG *F AAMTYLHSMGIVHRDIKPENLLVKLDEHGNVLELKLADFGLACEVNDLLYAVCGTPTYVAPEILLEVGYGLKIDVWAAGI *F ILYILLCGFPPFVAPDNQQEPLFDAIISGIYEFPDPYWSDIGDGVRDLIANMLQADPDVRFTSEDILDHSWTIGNKGNEC *F TTYKR *F >CG11573.AA *F MESGAIADFQITASSAHDMGNVGPQHARLKIDNNGGAWCPKHMVSRGLTEYLQVDLLGVHLVSAIRTQGRFGKGQGQEYT *F EAYVIEYWRPGLKKWIRWRSLQGKEVLPGNINTYSEVENVLQPSVFASKVRLYPYSQYDRTVCLRAEIVGCAWEEGIVSY *F SIPKGMQRGMDIDLSDKTYDGYEEGDHYVNGLGQLVDGQRGKDNFRADINGLGKGYEWIGWRNDTLLGRPVEIIFEFETV *F RNLSAVIIHTNNMFSKDVQVFVHAKVFFSIGGRHFSGEPVQFSYMPDLVLDHARDVTIKLHHRLGRYVQLHLYFAARWMM *F ISEITFISAPVVGNFTDELANGEKQSVTSEYPLQRDEVGRAISSGGDLLILLIQLLLPLIINVCSTPFMRSSHLPKSPGR *F GSSPEFVVATGKVTASARNSLNAATLPLPSPPVPPPLEKYYAATPVSSKPMPVAPPGSQSSGSLSSSTTAATTPTSGVVG *F VGKPHHYNLDMSANFADINEERANCQVQEFPRQSLVIVEKLGSGVFGELHLCETNVLNDLTEFDSHCSATLVAVATLRPG *F ANDHLRKEFRSKAKQLAQLSDPNVARLVGACLRDEPICIVQDYSHCLGDLNQFLQEHVAETSGLMAKKSLSFGCLVYIAT *F QIASGMKHLEQMNFVHRDLATRSCIIGPELCVKVCSIGTVINRSAYASDYCQLEGFTGRQSQPMPIRWMAWESVLLGKFT *F TKSDVWSFAVALWEILTFAREQPYEHLSDKSVIENIGLIYRDYKMHELLPMPPNCPREIYDLMCECWQRDESSRPSFREI *F HLYLQRKNLGFKPQTHTHMY *F >gek.AA *F MEYESSEISDITTGSCKKRLTFLKCILSDTTSDQKWAAEFGEDTEGHQFSLDYLLDTFIVLYDECSNSSLRREKGVSDFL *F KLSKPFVHIVRKLRLSRDDFDILKIIGRGAFGEVCVVQMISTEKVYAMKILNKWEMLKRAETACFREERDVLVFGDRQWI *F TNLHYAFQDNINLYLVMDYYCGGDLLTLLSKFEDKLPEDMAKFYITEMILAINSIHQIRYVHRDIKPDNVLLDKRGHVRL *F ADFGSCLRLDKDGTVQSNVAVGTPDYISPEILRAMEDGKGRYGTECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNH *F QNCFNLPSQETLNYKVSETSQDLLCKLICIPENRLGQNGIQDFMDHPWFVGIDWKNIRQGPAPYVPEVSSPTDTSNFDVD *F DNDVRLTDSIPPSANPAFSGFHLPFIGFTFSLTSSSTLDSKKNQSSGFGDDTLDTISSPQLAILPSNNSETPVDSVQLKA *F LNDQLAALKQEKAELSKQHNEVFERLKTQDSELQDAISQRNIAMMEYSEVTEKLSELRNQKQKLSRQVRDKEEELDGAMQ *F KNDSLRNELRKSDKTRRELELHIEDAVIEAAKEKKLREHAEDCCRQLQMELRKGSSSVETTMPLSISSEMSSYEIERLEL *F QFSEKLSHQQTRHNMELEALREQFSELENANLALTKELQQTQERLKYTQMESITDSAETLLELKKQHDLEKSSWFEEKQR *F LSSEVNLKSKSLKELQAEDDEIFKELRMKREAITLWERQMAEIIQWVSDEKDARGYLQALATKMTEELEYLKHVGTFNNN *F GVDNKNWRNRRSQKLDKMELLNLQSALQREIQAKNMISDELSQTRSDLISTQKEVRDYKKRYDSILHDFQKKETELRDLQ *F KGGLEYSESFLNKSTHHGLSSAFFRDMSKNSEIIDSAESFGNESGDNFTPNFFQSGNSGMLFNYEPKYAGKNNKDHSSMK *F EASVSDLSREESDQLVKESQKKVPGNTAIHQFLVRTFSSPTKCNHCTSLMVGLTRQGVVCEICGFACHTICCQKVPTTCP *F VPMDQTKRPLGIDPTRGIGTAYEGYVKVPKSGVIKRGWIRQFVVVCDFKLFLYDISPDRCALPSVSVSQVLDMRDPEFSV *F GSVRESDVIHAAKKDVPCIFKIKTALIDGGLSLNTLMLADNESEKSKWVIALGELHRILKRNSLPNTAIFKVNEILDNTL *F SLIRNALCSVIIYPNQILLGTEDGLFYINLDQYEIARIGESKKILQLWYIEEEQILVILCGKQRNLRLLPIRALEASDVE *F WIKVVESKNCISACTGIIRRFPNIVYSFIIALKRPNNHTQIVVYEINRTRTRHQKTCEFTIGYMAQHLQILSDMRLVVAH *F QSGFTAYFLRGEATAMSLVHPENQLCAFLNYSGVDAVRVIEILCPSGGNFGEYLLVFQTLAIYVDLQGRKSRDREIMYPA *F FPTYITFCDGHLLVFSDTHLDIFNTQTAEWVQSIGLKQSLPLNNLGNVVLSSVNDTPLIVYLSNIHTKGLLQYRDGNRKG *F LPSIKRRFSIREINKTIKSDRRSKMISAPTNFNHISHMGPGDGIQNQRLLDLPTTLETADQACSPIIHSLSCIPQSRKSN *F FLEQVDANSDDYGNDNIISRTPSPMASSFMDGLSNND *F >rok.AA *F MPAGRETVTKQRSMDVERRRRANTLEREMRDPTSICNVDCLLDTVSALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQL *F RMNVEDFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQLHFAFQDAKYL *F YMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNMLLDSYGHLKLADFGTCMRMGANGQV *F VSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLYEMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQA *F KALIRAFLTDRTQRLGRYGIEDIKAHPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFDG *F NHLPFIGFTYTGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRHRPSNSNELKRLEALLERERGRSEALEQQDAG *F LRQQIELITKREAELQRIASEYEKDLALRQHNYKVAMQKVEQEIELRKKTEALLVETQRNLENEQKTRARDLNINDKVVS *F LEKQLLEMEQSYKTETENTQKLKKHNAELDFTVKSQEEKVRDMVDMIDTLQKHKEELGQENAELQALVVQEKNLRSQLKE *F MHKEAENKMQTLINDIERTMCREQKAQEDNRALLEKISDLEKAHAGLDFELKAAQGRYQQEVKAHQETEKSRLVSREEAN *F LQEVKALQSKLNEEKSARIKADQHSQEKERQLSMLSVDYRQIQLRLQKLEGECRQESEKVAALQSQLDQEHSKRNALLSE *F LSLHSSEVAHLRSRENQLQKELSTQREAKRRFEEDLTQLKSTHHEALANNRELQAQLEAEQCFSRLYKTQANENREESAE *F RLSKIEDLEEERVSLKHQVQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFVMKHRNEINAKEAALATLKEAEN *F ELHKKLGQKAAEYEDLVQQHKKQQEELALMRSSKDEEITKLLDKCKNEVLLKQVAVNKLAEVMNRRDSDLPKQKNKARST *F AELRKKEKEMRRLQQELSQERDKFNQLLLKHQDLQQLCAEEQQLKQKMVMEIDCKATEIENLQSKLNETASLSSADNDPE *F DSQHSSLLSLTQDSVFEGWLSVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGD *F VIRADAKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNCNEERPGTIVHKGHEFVHITYHMPTACEVCPKPLWHMFKP *F PAAYECKRCRNKIHKEHVDKHDPLAPCKLNHDPRSARDMLLLAATPEDQSLWVARLLKRIQKSGYKAASYNNNSTDGSKI *F SPSQSTRSSYKPYAVNVQRSATLPANSSLK *F >CG10522.AA *F MPPKMEPISVRTARLNNLILGKGAGVCAKPAGSASGSGIPASTRRSIVPVSTTSAAVAEAICREGLLDAFCLLYNECDKD *F TLKKRDRNIAEFVNKFRPIIEETRKLRVNADDFLIKTLIGQGYFGNVHLVVERQTNDIYAMKKIKKSVVTTSQVKEERDI *F MSIRNSEWLINLQYAFQDNDNLYLVMEYMPGGDLLSLMSRHGPFDEDLARFYLAELTVALHTLHEMGYVHRDIKPENILI *F DRFGHIKLADFGNAAALDRDGHVLSLSPVGTPDYIAPELLQTISTYKLSKSMHDVSCDYWSMGIIGYELICETTPFHEDN *F VHETYSKILSHCEESHLKELISFPADLKVSVNYRNLIESLVTNPSKRLSYERIKNHPFFSEIPWGSIRSQVPPIIPTVRS *F DDDTSNFEDGIRHKTRREQGVAKKSLTTNMKSNDFSGKDLPFIGYSFVHMEKSAISATTDEKLQEKLKELLQKLKTRENE *F ISMLKQDLLRAQQSLRKTDNKSQVVADAKMEIKKLQQIIKEKTMELTTCKTQIKTLQSSAKIDEEMWSKKEATITDLLRL *F NRQKYEEAKIASEQRYEKQLADKKQELASTLQKLDARELEFNAKFEECKHLSMKLQNYKDMLQQIKEQNLKSETNHEEQR *F RQMAELYEQKLTDLRKKVRDSQDTNRRMTMEIKEIRTELDESISSSKSTQEAKNATERNIEEILRRLNEEIASNNELHAE *F KVKLETKLQLKENETQEVRAECHRLERELQLAECRCQLAESSLATQVSPYETAPGSLTELNAIEDQLRADLLAAKESENH *F QKGRADQLQTLVTKLEQMLERFNEQSLSPTKSHSSRKQEGETVGDMLERQNEKLEDKLAAVREQMIVERQAARTANLSLW *F KVEKQLEEALSEKKLLARRMELTEDRIKKVQNASDEAQRMLKTSQEETRQRESRIEELKQELAAAKRDVLKEHRQWEKAE *F QERMKCKSEIIEHLANVHRLEQQETELRQKLRQIQSRFDGVTLEQKNTIRELQEEREKSRKANDSCLVLQKELKQLTDNF *F QRLKYACSITDSQLTEVETMLKSEQERNKSQKSQLDTLHEKLRERNDQLTDLRKQLTTVESEKRLAEQRAQVLASEIDEL *F RLNLKEQQKKLVAQQDQLVEQTNALFATQERAELLDGQNANYEAQTADSNREMVSLKEENARILSELFHKKEEVGNLQAE *F IRGLESAQANLHAEIDSLQDTLAEKEQFYVQRDIKSNATLAQHKKLIDYLQV *F >CG17090.AA *F MKTSYPPKASDFVDYAVAGGGGSGFCSGLGAGLSVRATSFYGDQTVTATTDGTRHQEQQQQQLQQQQQQHILRQPATSAS *F TSSVAAAATSKRKRQTDCDCGCVDSYNSSHGPPVQQDSSAAAGAGSVGSKAGSGPGPDGIGPISSLKTAHTKVATSGGHA *F NTQPPSKRSSSGADGDYQLVQHEVLYSLSAEYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSIL *F SRLSQENADEFNFVRAFECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILEQVLTALLKLKQLGLIHADLKP *F ENIMLVDPVRQPYRVKVIDFGSASHVSKTVCNTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVVAELFLGWPLYPGSSEF *F DQIRYISQTQGLPTEHMLNSASKTSKFFYRDVDSTYPFWRLKTTEEHEAETNTKSKEARKYIFNCLDDIGQVNVPTDLEG *F GQLLAEKTDRREFIDLLKRMLTIDQERRLTPAEALNHSFTRLTHLVDYVYCNNVKASVQMMEVCRRGDFHTVQPASTLVT *F NFVPSSTENMTFTINNQLTSQVQRLVRDGRPLAYEGLYQIYNGRSVARQYPQTRTDSFQHQLVSNILCPPSYQTMPSPTK *F HVVVGSATMQPPLQVPPQQYVNVPVPVSMVEPTSGQRMLLTNRVQASGVAWPQTGRQMALVPSWPQQAPAHSLIVDSTPL *F FNVEEIYPKHHLNLPRNDLKKESPAHHIAKGNSYRVPRHEKKEHQQLSPVKKRVKESSPPHQQRYQRAAHVSPQYHAHHN *F CNYGNGSGYSASAGSVVASSAASSSNIVNGSSSSSHHHHVPVAHAQPYSSSSGHHIVSNCNANGGAGGAVVYQQPPAHAH *F QQHGQQPHPQSSQHPQHVKQPTITIHDTPSPTAVITISDSEDEGGEAGGAQVPVLKQRAHAQSQTSGSLLQHAPSSSCSR *F SSAHQQPHPHPQQQQQQQINYGDHDPEDARRRHHAAAAAAQQQQQYQPQPPPQQVLPQPQPSIKYEPGQSQKKRILAMAQ *F SECGYQPQVPSQPSSSASLPHIPTKQEPAEFYPEYAAAPPQQLDTKRSSWAPTSSGSGVSALPLAHPKREAPSVAPISYV *F APSVAPPLAHSKSSSASSSSSISAATAAAAAAAAAAAASVGPPSWGPPQVYRQPSQPPPGSVGLPGSTQPPPSSVAPHPH *F HHSHGHHHHQAGTPLGGSPSSTAAAALLQPDIYAQGDIYRRPTVFVSQAAPSYAYANRAVVAPPPAHNSSSRQVIPSHPL *F PAHIQIPTQYSQFGPLSPAQVAASKHAAHFAPTNIWYGAE *F >Dyrk3.AA *F MINQNLTHTGIAQNNTEKANRHQYRDSGLQYLTRCFEPLAMLNDSKEDFPTQPSNNIANYPGDIQILPIFDCCEISESIQ *F AISLPNVTSPSKTKDVPGLFLRTISENSKSKSEPECESLISVKESSVMENHTFLFHEQIIMSGQQKCELHEKPKVLVVSP *F QQVMILYMNKLTPYERTEILTYPQIYFIGANAKKRPGVYGPNNSEYDNEQGAYIHVPHDHVAYRYEMLKIIGKGSFGQVI *F KAYDHKTHEHVALKIVRNEKRFHRQAQEEIRILHHLRRHDKYNTMNIIHMFDYFTFRNHTCITFELLSINLYELIKKNGF *F KGFSLQLVRKFAHSLLQCLDALYKNDIIHCDMKPENVLLKQQGRSGIKVIDFGSSCFENQRIYTYIQSRFYRAPEVILGG *F KYGRAIDMWSLGCILAELLSGHALFPGENESDQLACIIEVLGMPNKNILASSKRSKSFFSPKGYPRYCTVRTMSDGMVVL *F IGGQSRRGKQRGPPCSKSLSKALDGCKDPLFLNFIRGCLEWDADKRLTPSEALKHPWLRRRLPRPPSSSSGCGGVSGLCS *F SRNESPVTGKLDL *F >mnb.AA *F MHHHSSPSSSSEVRAMQARIPNHFREPASGPLRKLSVDLIKTYKHINEVYYAKKKRRAQQTQGDDDSSNKKERKLYNDGY *F DDDNHDYIIKNGEKFLDRYEIDSLIGKGSFGQVVKAYDHEEQCHVAIKIIKNKKPFLNQAQIEVKLLEMMNRADAENKYY *F IVKLKRHFMWRNHLCLVFELLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLSTPELNIIHCDLKPENILLCNPKR *F SAIKIVDFGSSCQLGQRIYHYIQSRFYRSPEVLLGIQYDLAIDMWSLGCILVEMHTGEPLFSGCNEVDQMNKIVEVLGMP *F PKYLLDQAHKTRKFFDKIVADGSYVLKKNQNGRKYKPPGSRKLHDILGVETGGPGGRRLDEPGHSVSDYLKFKDLILRML *F DFDPKTRVTPYYALQHNFFKRTADEATNTSGAGATANAGAGGSGSSGAGGSSGGGVGGGLGASNSSSGAVSSSSAAAPTA *F ATAAATAAGSSGSGSSVGGGSSAAQQQQAMPLPLPLPLPLPPLAGPGGASDGQCHGLLMHSVAANAMNNFSALSLQSNAH *F PPPSLANSHHSTNSLGSLNHISPGSTGCHNNNSNSSNNNTRHSRLYGSNMVNMVGHHNSGSSNNHNSISYPHAMECDPPQ *F MPPPPPNGHGRMRVPAIMQLQPNSYAPNSVPYYGNMSSSSVAAAAAAAAAAASHLMMTDSSVISASAAGGGQGGGNPGQN *F PVTPSAAAFLFPSQPAGTLYGTALGSLSDLPLPMPLPMSVPLQLPPSSSSSVSSGSASVGSGGVGVGVVGQRRHITGPAA *F QVGISQSVGSGSSGSATGASSSDASSSSPMVGVCVQQNPVVIH *F >smi35A.AA *F MLDRCEMPIQLDNEKLRRDVRLSGSRLDLPQLCNGSRRLDGHNNHVAANENTVTTTSLNGNGNGNGNSNSNNNNNIGSPV *F SSSTTNSSNGGNERGSSTKSNSSSGSGSSGNSASSTGSGELKCNTPMTPSELVKKFRNYLTDLEFEELKVYKEVWYFGQH *F ASKNYNKPAPTANTTNLGYDDDNGNYKIIEHDHIAFRYEILEVIGKGSFGQVIRALDHKTNTHVAIKIIRNKKRFLNQAV *F VELNILDELREKDADGSHNVIHMLDYTYFRKHLCITFELMSLNLYELIKKNNYNGFSMSLIRRFCNSIVKCLRLLYKENI *F IHCDLKPENILLKQRGSSSIKVIDFGSSCYVDRKIYTYIQSRFYRSPEVILGLQYGTAIDMWSLGCILAELYTGFPLFPG *F ENEVEQLACIMEVLGLPPKVLISVARRRRLFFDSRDAPRCITNTKGRKRSPGSKSLAHILHCQDRYFIDFLQRCLEWDPA *F ERMTPDEAAHHEFLQPSASSRHRSCRMSSSSSSSGLNSVSQKSSCYSFSEISPGTNGPVVASITSTTAVHNAAIATTTKS *F RQQPPSQSHGHAQSNGHLPDIKLSASDKYNSMQKVAVRSKITSSVSDLESVQQYSLHRIYGGVGSGSTHHVSSAATRKHL *F PGTGSGIVGAMSSKYGSSTLAHNHHNVTHHNASTATIATTTHHHHHHGGQQQQQSSSGASTMAMSHSQSTGDVSDRAIFG *F RA *F >BcDNA:GH04978.AA *F MADDKSYDSSNNSETEERRAKHKKAKKHKKHKKSSTGKTEKDRHVHKKQKKAKKRPHRTSESTNESDAPENAKLKLSQNC *F GLSSKFTEIMQKASRNGADFRVGKPLLPTDPSSLVEEITKTIQNKMLPVLEVASSSSESDVPADAASPIILSIIEDELNL *F EELMRQKALLQARLGAYMSDPEAEDKGDLLPHTHSQQLVVHTKSVLKAVTSVPQPQPSKSAAPLATATSKHANIKKSTTH *F NSNESDVILLDDSSGGQRTPSPTPEKRRRHASPLPAAPRSRCRRDRRSRDRDRSPPRRRASDQQTQVRPRSRNRNMEDLR *F QEINRDKQRERRDHSRDRDRRGLDRPPPPTDMRPGRARERDVRPSRMQRSHSRSKFESDRRRERERQKDRDRGGGRVGAR *F SGTDNGDRDRYKGSLSEGQNKLDKESSDEEVNVNIDILEEDDEERIIELRRKKREELLKKLGTGQESPAPHNSVSYESRS *F TSQGSSQRERPLRTPSPTMPCPNPLISEIPKDREDMDNSTSQKTCSAKEKRNEWDMFADQDVDSNFDSPNTIVQNKHQHE *F NPALTDNWDDAEGYYRVRIGEVLDNRYLVNGYTGQGVFSNVVRGRDQARGQANVAIKIIRNNEIMHKTGLRELEILKKLN *F DADPEDRFHCLRLYRHFFHKQHLCMVFEPLAMNLREVLKKYGKNVGLHIKAVRSYTQQLFLALKLLKKTGILHADIKPDN *F ILVNENNLILKLCDFGSASAISDNEITPYLVSRFYRSPEIILGIPYDYGIDTWSAGCTIYELYTGKILFSGKSNNQMLKF *F FMDVKGKIPNRIIRKGQFREQHFDQSCNFLYHEIDKLTEREKIVVMPVVKPSRSLQQELIADQNLPDDQHRKVTQLKDLL *F ENMFALDPAKRISLNQALVHPFIQEKM *F >Egfr.AA *F MLLRRRNGPCPFPLLLLLLAHCICIWPASAARDRYARQNNRQRHQDIDRDRDRDRFLYRSSSAQNRQRGGANFALGLGAN *F GVTIPTSLEDKNKNEFVKGKICIGTKSRLSVPSNKEHHYRNLRDRYTNCTYVDGNLELTWLPNENLDLSFLDNIREVTGY *F ILISHVDVKKVVFPKLQIIRGRTLFSLSVEEEKYALFVTYSKMYTLEIPDLRDVLNGQVGFHNNYNLCHMRTIQWSEIVS *F NGTDAYYNYDFTAPERECPKCHESCTHGCWGEGPKNCQKFSKLTCSPQCAGGRCYGPKPRECCHLFCAGGCTGPTQKDCI *F ACKNFFDEGVCKEECPPMRKYNPTTYVLETNPEGKYAYGATCVKECPGHLLRDNGACVRSCPQDKMDKGGECVPCNGPCP *F KTCPGVTVLHAGNIDSFRNCTVIDGNIRILDQTFSGFQDVYANYTMGPRYIPLDPERLEVFSTVKEITGYLNIEGTHPQF *F RNLSYFRNLETIHGRQLMESMFAALAIVKSSLYSLEMRNLKQISSGSVVIQHNRDLCYVSNIRWPAIQKEPEQKVWVNEN *F LRADLCEKNGTICSDQCNEDGCWGAGTDQCLTCKNFNFNGTCIADCGYISNAYKFDNRTCKICHPECRTCNGAGADHCQE *F CVHVRDGQHCVSECPKNKYNDRGVCRECHATCDGCTGPKDTIGIGACTTCNLAIINNDATVKRCLLKDDKCPDGYFWEYV *F HPQEQGSLKPLAGRAVCRKCHPLCELCTNYGYHEQVCSKCTHYKRREQCETECPADHYTDEEQRECFQCHPECNGCTGPG *F ADDCKSCRNFKLFDANETGPYVNSTMFNCTSKCPLEMRHVNYQYTAIGPYCAASPPRSSKITANLDVNMIFIITGAVLVP *F TICILCVVTYICRQKQKAKKETVKMTMALSGCEDSEPLRPSNIGANLCKLRIVKDAELRKGGVLGMGAFGRVYKGVWVPE *F GENVKIPVAIKELLKSTGAESSEEFLREAYIMASVEHVNLLKLLAVCMSSQMMLITQLMPLGCLLDYVRNNRDKIGSKAL *F LNWSTQIAKGMSYLEEKRLVHRDLAARNVLVQTPSLVKITDFGLAKLLSSDSNEYKAAGGKMPIKWLALECIRNRVFTSK *F SDVWAFGVTIWELLTFGQRPHENIPAKDIPDLIEVGLKLEQPEICSLDIYCTLLSCWHLDAAMRPTFKQLTTVFAEFARD *F PGRYLAIPGDKFTRLPAYTSQDEKDLIRKLAPTTDGSEAIAEPDDYLQPKAAPGPSHRTDCTDEIPKLNRYCKDPSNKNS *F STGDDETDSSAREVGVGNLRLDLPVDEDDYLMPTCQPGPNNNNNINNPNQNNMAAVGVAAGYMDLIGVPVSVDNPEYLLN *F AQTLGVGESPIPTQTIGIPVMGVPGTMEVKVPMPGSEPTSSDHEYYNDTQRELQPLHRNRNTETRV *F >Eph.AA *F MSLLRTILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKGINWRSYVVCDVAYHNVNN *F WLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDAATREPPPWQTDSYRLIARIAAGEGRFNQNSD *F VDINTEVKSIAVNKKGVYFAFRDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPT *F YLCKGDGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHM *F PCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSPNVVYNPSTDTFNETKITLTNL *F EPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILA *F ITNKDADLEWDKPVQSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYA *F QTLQSVGSVYDDSVQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDTTPIVKKL *F HLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTR *F SNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFGLSREI *F ENASDAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEA *F LYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSLLTTRPSPESDGNHILDGQRGQNIFISTDLWLEHIKMSRYCHHFK *F EANLINAQQVNSSNYHFCINQFFIVKY *F >Fak56D.AA *F MNTAGATSQPPPTKNEINSEEYLIHVHMPNKSFKAVRFNVKETVFHVIRRTVEDLGTDGRTPSIQRYACRMLNMITKEVI *F WLARSTSMQKVLSHILTPGCSNVDCPNNQSELDEVLLEHGRRITDNRVWRVELRVRYVPNNIQELFEEDKATCFYYFNQV *F KEDFIQANVTAIDTEVAVQLCCLGIRHYFKNITVKAPDKKQHIDYIEKEIGFKSFLPQSVIATSKPKNLKKLIQVGYKKV *F YNYNDIEYLTRFFDLLKNIYLTNFEQFSVTLSSAWNISGILHVGPHIGISYQTHPQASLKNVAQFKDVVSIKTCTLPKEK *F LSKSGENTTEPELQNFNCNCQKIKTQIKISASNNVEDLVITCNGINTAESIADLIDGYCRLLSKDLEFTIWHRETNASNE *F DSAKALPNDATLGSNKSTSSQGKPMLTDDYAEIGLLEGEGDYSTPTVRNYELDRALITPSAKIGVGQFGDVYVGTYTLPK *F LGKGKNLAGNGKNSNSDQRNADSRPDVIQVAIKTCKANDDPEKTENFLAEAYIMQKFDHPHIIRLIGICSVMPIWIVMEL *F AKLGELRAYLKTNSERLSHGTLLKYCYQLSTALSYLESKKFVHRDIAARNVLVSSPTCVKLADFGLSRWVSDQSYYHSTP *F TVALPIKWMSPESINFRRFTTASDVWMFGVCIWEILMLGVKPFQGVKNSDVILKLENGERLPLPPNCPPRLYSLMSQCWA *F YEPLKRPNFKRIKETLHEILIEDSINSSETLKREQRKVASMSWIGSDDIDIPPSKPSRVMHDPDITGLMPETTGLPQTYI *F IAQNPAVLAKLMMENQKRGINPAAYTTPASGIHNVLGEKLRQQQKDSNSDSEWLIQEELLRQRSCSIPQGSLNDHQAQMF *F KLDFMSAGPSSLPDCSNSSSRPMTPNANLSSLKSNHSSADHLSSLTSAEEQMGSNARNLGSAVPSRPPNRADDEVYCATT *F LVVKSIMALSQGVEKANTEGYLELVKNVGVKLRNLLTSVDKISIIFPAQALKEVQMAHQVLSKDMHELVSAMRLAQQYSD *F TTLDCEYRKSMLSAAHVLAMDAKNLFDVVDSIRQRYQHLFPPSATKETSCSSSFESTSGSIVAEPVNDLGGYIKTSTSGD *F LLQNTGIYDNDLHHSFNSQLQLQNPKASIDLSGGGSLQRGMSLGLDTTRSTNEPLRIVEETLGSPGEHMYCNTSALHGHA *F *F >Fps85D.AA *F MGFSSALQSRAAHEALIVRQDAELRLMETMKRSIQMKAKCDKEYAISLTAVAQQGLKIDRADEMQGSLISKSWRSYMDEL *F DHQAKQFKFNAEQLEVVCDKLTHLSQDKRKARKAYQEEHAKIAARLNHLTDEVVRKKSEYQKHLEGYKALRTRFEENYIK *F APSRSGRKLDDVRDKYQKACRKLHLTHNEYVLSITEAIEVEKDFRNVLLPGLLEHQQSVQESFILLWRNILQEAAQYGDL *F TADKYKEIQKRIDTVIGSINPTEEYGEFTEKYKTSPTTPLLFQFDETLIQDIPGKLQSSTLTVDNLTVDWLRNRLQELEG *F AVRDCQEKQMKMIEHVNGGSPVANGSIISNGSNTSNGIQSNKDSLCRQSKDLNALRCQEKQKQKLVDMIKCALNEVGCEE *F LPSGCDDDLTLEQNFIENGYNNEQQISLSTNRPLYEEEWFHGVLPREEVVRLLNNDGDFLVRETIRNEESQIVLSVCWNG *F HKHFIVQTTGEGNFRFEGPPFASIQELIMHQYHSELPVTVKSGAILRRPVCRERWELSNDDVVLLERIGRGNFGDVYKAK *F LKSTKLDVAVKTCRMTLPDEQKRKFLQEGRILKQYDHPNIVKLIGICVQKQPIMIVMELVLGGSLLTYLRKNSNGLTTRQ *F QMGMCRDAAAGMRYLESKNCIHRDLAARNCLVDLEHSVKISDFGMSREEEEYIVSDGMKQIPVKWTAPEALNFGKYTSLC *F DVWSYGILMWEIFSKGDTPYSGMTNSRARERIDTGYRMPTPKSTPEEMYRLMLQCWAADAESRPHFDEIYNVVDALILRL *F DNSH *F >btl.AA *F MAKVPITLVMIIAIVSAAADLGCDYGHHRCYIDVTVENSPRQRHLLSDMDITLQCVRPMAKWFYEDKFQLRATLLRLERA *F QSGNSGNYGCLDSQNRWYNISLVVGHKEPVGNDIASFVKLEDAPALPESDLFFQPLNESRSLKLLQPLPKTVQRTAGGLF *F QLNCSPMDPDAKGVNISWLHNDTQILGGRGRIKLKRWSLTVGQLQPEDAGSYHCELCVEQDCQRSNPTQLEVISRKHTVP *F MLKPGYPRNTSIALGDNVSIECLLEDSALEPKITWLHKGNADNIDDLLQRLREQSQLPVDVTRLITRMDEPQVLRLGNVL *F MEDGGWYICIAENQVGRTVAASYVDLYSPSDTTTVRTTTTTTVASPIPTASTGEDNDDDVENPAAEASGGVGPPVFRKEL *F KRLQHSLSGNTVNLACPVYGKANITWTKDKKPLNRELGVYVQKNWTLRFVEATSEDSGLYNCKVCNAWGCIQFDFSVQIN *F DRTRSAPIIVVPQNQTVKVNGSLVMKCTVYSDLHPTVSWKRVVLKNASLDGLKSVEIQNLNFTVTNDSVVLTLRNVTFDQ *F EGWYTCLASSGLGRSNSSVYLRVVSPLPPLEIYALLHAHPLGFTLAAITIVALFLLGSAFITFMLRRLRREKLLKLRIET *F VHQWTKKVIIYRPGGEEGSGCSSGDLQMPVIRIEKQRTTVSTTGTGGTDPAQGFNEYEFPLDSNWEIPRQQLSLGSILGE *F GAFGRVVMAEAEGLPRSPQLAETIVAVKMVKEEHTDTDMASLVREMEVMKMIGKHINIINLLGCCSQGGPLWVIVEYAPH *F GNLKDFLKQNRPGAPQRRSDSDGYLDDKPLISTQHLGEKELTKFAFQIARGMEYLASRRCIHRDLAARNVLVSDGYVMKI *F ADFGLARDIQDTEYYRKNTNGRLPIKWMAPESLQEKKYDSQSDVWSYGVLLWEIMTYGDQPYPHILSAEELYSYLITGQR *F MEKPAKCSLNIYVVMRQCWHFESCARPTFAELVESFDGILQQASSNPNDAYLDLSMPMLETPPSSGDEDDGSDTETFRET *F SPLRYQYTYKFN *F >htl.AA *F MAAAWSWRASHSTITMTSGSLVVLFLLLSIWQPAVQVEGRRQMANSQEMIKDHLGARSQNKTPAITNNANQSSTSSADLD *F DGAADDDDNKADLPVNVSSKPYWRNPKKMSFLQTRPSGSLLTLNCHALGNPEPNITWYRNGTVDWTRGYGSLKRNRWTLT *F MEDLVPGDCGNYTCKVCNSLGCIRHDTQVIVSDRVNHKPILMTGPLNLTLVVNSTGSMHCKYLSDLTSKKAWIFVPCHGM *F TNCSNNRSIIAEDKDQLDFVNVRMEQEGWYTCVESNSLGQSNSTAYLRVVRSLHVLEAGVASGSLHSTSFVYIFVFGGLI *F FIFMTTLFVFYAIRKMKHEKVLKQRIETVHQWTKKVIIFKPEGGGDSSGSMDTMIMPVVRIQKQRTTVLQNGNEPAPFNE *F YEFPLDSNWELPRSHLVLGATLGEGAFGRVVMAEVNNAIVAVKMVKEGHTDDDIASLVREMEVMKIIGRHINIINLLGCC *F SQNGPLYVIVEYAPHGNLKDFLYKNRPFGRDQDRDSSQPPPSPPAHVITEKDLIKFAHQIARGMDYLASRRCIHRDLAAR *F NVLVSDDYVLKIADFGLARDIQSTDYYRKNTNGRLPIKWMAPESLQEKFYDSKSDVWSYGILLWEIMTYGQQPYPTIMSA *F EELYTYLMSGQRMEKPAKCSMNIYILMRQCWHFNADDRPPFTEIVEYMDKLLQTKEDYLDVDIANLDTPPSTSDEEEDET *F DNLQKWCNY *F >Gprk2.AA *F MELENIVANTVYLKAREGGSDSNKGKSKKWRKILQFPHISQCINLKDKLDISYGYVIDQQPIGRELFRLFCENKRPVYFR *F YITFLDEVVKYEIEYISNRIFIGHDIGRRFLDVEAQLELRNGSGGDALDAETQEELLLNSSNANPTETAETEHCNNTTAN *F NCNNINNSNNSQHSSDINHKKLDTRNHNGDDATGNGSSHQDDGDESVKCQEGHDDAEKGGGGEGGGGGKCVPVGGYPDEL *F VLDVLNDDLIAQVRNKLNSGGKDIFAQCVNAVKAFLAGEPFREFESSMYFHRYLQWKWLEAQPITYKTFRMYRVLGKGGF *F GEVCACQVRATGKMYACKKLEKKRIKKRKGESMVLIEKQILQKINSPFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYN *F MGGEPGFELERARFYAAEVACGLQHLHKQGIVYRDCKPENILLDDHGHVRISDLGLAVEIPEGEMVRGRVGTVGYMAPEV *F IDNEKYAFSPDWFSFGCLLYEMIEGQAPFRMRKEKVKREEVDRRVKEDPEKYSSKFNDEAKSMCQQLLAKSIKQRLGCRN *F GRMGGQDVMAHPFFHSTQLNWRRLEAGMLEPPFVPDPHAVYAKDVLDIEQFSTVKGVNIDESDTNFYTKFNTGSVSISWQ *F NEMMETECFRELNVFGPEECPTPDLQINAAPEPDKAGCFPFRRKKKQPARTQPIPIPEHLLTTSHSVSSTTVES *F >Gprk1.AA *F MADLEAVLADVSYLMAMEKSKCTPAARASKKLNLPDPSVRSVMYKYLEKEGELNFHKNFNEVLGYLLFKDFCENDSEEPI *F QQLKFFEQIKLFEKTECYDERKKMARDIYDNFIMEEMLSHTYEYSKHAVASVQKYLLKNEVPVDLFEPYLEEIFTQLKGK *F PFKKFLESDKFTRFCQWKNLELNIQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGEMLALNER *F NMLQAVSTGIDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGIFSEDEMKFYAAEVILGLEHMHKRCIVYRDLK *F PANILLDENGHIRISDLGLACDFSKKKPHASVGTHGYMAPEVLSKGTSYDSCADWFSFGCMLYKLLKGHSPFRQHKTKDK *F LEIDKMTLTMNVELPESFSLELKNLLEMLLQRDVSKRLGCMGNGADEVKMHNFFCGIDWHQVYIQKYTPPLVPPRGEVNA *F ADAFDIGSFDEEDTKGIKLNDADQDLYKMFSLTISERWQQEVSETVFDTVNTETDKLEQKRKLKQKQHFDADEKESDCIL *F HGYIKKLGGSFASLWQTKYAKLYPNRLELHSESGNNKPELIFMDQVEDISSDFILHKNENCIQIRINDGTRDGRIILTNS *F DEIGLKEWSSSLRSAHKISQDLLGSMAKKAGKIYGSERDVNKSMYIFGGNCSTKTSNGSN *F >gskt.AA *F MASQSKNSGLTNKVTTVVATNAFGADVMSEISYTDAKVVGNGSFGVVFQAKMVPSNEMVAIKKVLQDRRFKNRELQIMRK *F LRHDNIITLKWFFFSSGEKRDEVYLNLVMEFLPETLYKVERQYARAKQTLPVNFVRLYMYQLLRSMGYLHSLGFCHRDIK *F PQNMLLDSETGVLKLCDFGSAKQLISGEPNVSYICSRYYRAPELIFGSTDYTTKIDMWSAGCVMSELLLGQLIFPGDSGV *F DQIVEIVKVMGTPTSEQLHDMNPHYKQFKLPELKPHPWSKVFRIRTPAEAIDLVSKMLIYSPNARVSPLMGCAHPFFDEL *F RQDPHQQLPNGRSLPPLFNFTDYEKTIEPDTMPLLLPRAQGSSTTKEPSAAHRNRNTAGEESPRKTEDSQKPATAALSKS *F PGPSGKALESPPGFLQHDLGNGDHVAVGTMPMEPLTLEQNHFAAESYAVGEDAEDNLEEDVGDENDYDYDDGGQCNSTYI *F SDDMDEASESDDDDFEEEDEN *F >sgg.AA *F MFTFYTNINNTLINNNNNNNNTSNSNNNNNNVISQPIKIPLTERFSSQTSTGSADSGVIVSSASQQQLQLPPPRSSSGSL *F SLPQAPPGGKWRQKQQRQQLLLSQDSGIENGVTTRPSKAKDNQGAGKASHNATSSKESGAQSNSSSESLGSNCSEAQEQQ *F RVRASSALELSSVDTPVIVGGVVSGGNSILRSRIKYKSTNSTGTQGFDVEDRIDEVDICDDDDVDCDDRGSEIEEEEEDQ *F TEQEEEVDEVDAKPKNRLLPPDQAELTVAAAMARRRDAKSLATDGHIYFPLLKISEDPHIDSKLINRKDGLQDTMYYLDE *F FGSPKLREKFARKQKQLLAKQQKQLMKRERRSEEQRKKRNTTVASNLAASGAVVDDTKDDYKQQPHCDTSSRSKNNSVPN *F PPSSHLHQNHNHLVVDVQEDVDDVNVVATSDVDSGVVKMRRHSHDNHYDRIPRSNAATITTRPQIDQQSSHHQNTEDVEQ *F GAEPQIDGEADLDADADADSDGSGENVKTAKLARTQSCKNQTGRDGSKITTVVATPGQGTDRVQEVSYTDTKVIGNGSFG *F VVFQAKLCDTGELVAIKKVLQDRRFKNRELQIMRKLEHCNIVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAK *F TKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKLCDFGSAKQLLHGEPNVSYICSRYYRAPELI *F FGAINYTTKIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEVIKVLGTPTREQIREMNPNYTEFKFPQIKSHPWQKVFRIR *F TPTEAINLVSLLLEYTPSARITPLKACAHPFFDELRMEGNHTLPNGRDMPPLFNFTEHELSIQPSLVPQLLPKHLQNASG *F PGGNRPSAGGAASIAASGSTSVSSTGSGASVEGSAQPQSQGTAAAAGSGSGGATAGTGGASAGGPGSGNNSSSGGASGAP *F SAVAAGGANAAVAGGAGGGGGAGAATAAATATGAIGATNAGGANVTDS *F >Pk34A.AA *F MENKEKKQKPLKRHAQVPLIIPKNSVISTYAVGRLCSEPALVRIEVKDLIGSGSFGRVYQAHVNESEEIVAVKQTLYNPK *F LSQGEAEIMGQLKDHNNIVRLIMHSSVSLGFPSVDYVLLVMEYMPMTLLDYINYHLTVLQPAERLINVRILSYQMFRGLG *F YLHLLGISHRDVKPENLLIDNQKMVLKLSDFGSAKLLVPQEPSISYICSRLYRAPELFAGYELYSCAVDIWSAGCVLAEL *F LKGYPLFSSHKHDRKQLRLIVNMLGTDGLERAPEILSKCGNSLHPRTTRPSWNYLLNTAVPQDLCGLLNSCFIYEAAARI *F SPMMACSHGSYDELRIMDAMALPMPNGNPLPPLFDFNSLEMGTDPKLWVNLLPIHLSSMEDKYSVVEAFEAV *F >Haspin.AA *F MLSISKTKMDFLEEGRWKDPFDELLDSRTKLSKMNIVKQNVRVTYNIDSSVENSSYIEIKEPNHKNEPLTLEDCSIKVYC *F PSDSISTPCDKRLGGTTGLFETDLSPITRLKLEGVEDSRDKCADTNEADLYVNVEILFQNINSSPKKCSNFGKKRLSNLN *F KMVTAVHPSISLNPGKWRKSLNNFIRSKITETNFTKKVERRSSICQDRKSLVLKGEHKFENKYEEDVLKYCHQCTPLPFN *F TAYEQHKLLNTKKIGEGAYGEVFRCSRNQEVLKDHISDIVLKIIPLEGSTVINGEKQKTFSQILPEIIITKKMCSLRTSK *F TNSTNGFVSIQKVSLVKGRYPPHFIKLWEKYDNEKGSENDHPELFGDNQLFAVLELKFAGSDMANFKFLNSEQSYYALQQ *F IILALAVGEEEYQFEHRDLHLGNILIEYTNKKHIVCTFKSSNLTLLSKGVNVTIIDYTLSRVTINDCCYFNDLSRDEELF *F QATGDYQYDVYRMMRNELRNNWSSFSPKTNIIWLSYVIVKVLDSVKYKSINTKVHRMYIDKIKELKNIIMTFESASHCAN *F YLFNLN *F >ik2.AA *F MSFLRGSVSYVWCTTSVLGKGATGSVFQGVNKITGESVAVKTFNPYSHMRPADVQMREFEALKKVNHENIVKLLAIEEDQ *F EGRGKVIVMELCTGGSLFNILDDPENSYGLPEHEFLLVLEHLCAGMKHLRDNKLVHRDLKPGNIMKFISEDGQTIYKLTD *F FGAARELEDNQPFASLYGTEEYLHPDLYERAVLRKSIQRSFTANVDLWSIGVTLYHVATGNLPFRPFGGRKNRETMHQIT *F TKKASGVISGTQLSENGPIEWSTTLPPHAHLSQGLKTLVTPLLAGLLEENREKTWSFDRFFHEVTLILRKRVIHVFFTNR *F TSSVEVFLEPDEQIDNFRERIFLQTEVPLEKQILLFNNEHLEKKVTPRTIDQPIFLYSNDDNNVQLPQQLDLPKFPVFPP *F NVSVENDASLAKSACSVGHECKRRVDIFTSMDILIKKGVEHFIEMLVTTITLLLKKTESFDNLLSTVIDYADVVHSMARV *F TKGDQEIKTLLTALENVKSDFDGAADVISQMHKHFVIDDELNDQWTSSMHGKKCPCKTRASAQAKYLVERLRDSWQHLLR *F DRATRTLTYNDEQFHALEKIKVDHNGKRIKALLLDNVNPTVAQIAECLADWYKLAQTVYLKTQILEKDVRDCERKLNGIR *F DELYHVKSELKLDVDTKTINNNNQLAKIEERNRLRVMQQQQQEVMAVMRTNSDIISLLSKLGITNGSLESS *F >ird5.AA *F MITVVFCFSDCHGGIQILGRMSSVNKIKLNENNKMHSFGNWERCRNLGEGGFGLVIHWRNRTTGREIATKHIKEMGALSA *F DQQVKLSERWNKELNWSRQFKNFPHIVAGVDIEDPDFLEYLNGMFSAKLPVIVLEYCNGGDVRKRLQSPENANGLTEFEV *F RQILGALRKALHFLHSQCGICHRDLKPDNIVIQRGVDGKKIYKLTDFGLARGTPDQTMVQSVVGTRHYYAPEVVENGFYN *F STVDLWSFGVIAYELVTGELPFIPHQTLKNIILNLIKKPAKCIAITEDPEDNTRFVNQFELPQTHHLSRPWAAQFTKWLA *F SPLNSNYKERGQLAANNVPVVFADLDKILNMNVLTIFAVNNCERLEYAVSAEMTMKDLIALIVLDTGMDEKELYFVLPTS *F HPHKTITPKSTPLQLYVEEWSDTSKDSRKWTKRSNPPVMLYIFQVKKECDYKIPEPILSILSRKFIANKFKTKERWLQKR *F VVLDMLYVLTKEQARYEMLVSGINERALSLEDEMMENSFIDSIDKQRIIISFAYDQLTSLLKEAQAKIPSRQLISSAQWE *F KLNRNYNFIIQSAKSIRSFLEACLREAKDMVKTTNQLRKEVCEKDLFDCARFYKKYLCNGAIISPSELNNDAEEFAKSRF *F KLYNEGEARHLPKSIDHMHYLYFKTKESIPVLLQQFCDIKKEIFQINLQMLMSASSTPPPKLELSAAMDRLAISSGSPSS *F DPFDSLRTINAIEEAERINNM *F >InR.AA *F MFNMPRGVTKSKSKRGKIKMENDMAAAATTTACTLGHICVLCRQEMLLDTCCCRQAVEAVDSPASSEEAYSSSNSSSCQA *F SSEISAEEVWFLSHDDIVLCRRPKFDEVETTGKKRDVKCSGHQCSNECDDGSTKNNRQQRENFNIFSNCHNILRTLQSLL *F LLMFNCGIFNKRRRRQHQQQHHHHYQHHHQQHHQQHHQRQQANVSYTKFLLLLQTLAAATTRLSLSPKNYKQQQQLQHNQ *F QLPRATPQQKQQEKDRHKCFHYKHNYSYSPGISLLLFILLANTLAIQAVVLPAHQQHLLHNDIADGLDKTALSVSGTQSR *F WTRSESNPTMRLSQNVKPCKSMDIRNMVSHFNQLENCTVIEGFLLIDLINDASPLNRSFPKLTEVTDYIIIYRVTGLHSL *F SKIFPNLSVIRGNKLFDGYALVVYSNFDLMDLGLHKLRSITRGGVRIEKNHKLCYDRTIDWLEILAENETQLVVLTENGK *F EKECRLSKCPGEIRIEEGHDTTAIEGELNASCQLHNNRRLCWNSKLCQTKCPEKCRNNCIDEHTCCSQDCLGGCVIDKNG *F NESCISCRNVSFNNICMDSCPKGYYQFDSRCVTANECITLTKFETNSVYSGIPYNGQCITHCPTGYQKSENKRMCEPCPG *F GKCDKECSSGLIDSLERAREFHGCTIITGTEPLTISIKRESGAHVMDELKYGLAAVHKIQSSLMVHLTYGLKSLKFFQSL *F TEISGDPPMDADKYALYVLDNRDLDELWGPNQTVFIRKGGVFFHFNPKLCVSTINQLLPMLASKPKFFEKSDVGADSNGN *F RGSCGTAVLNVTLQSVGANSAMLNVTTKVEIGEPQKPSNATIVFKDPRAFIGFVFYHMIDPYGNSTKSSDDPCDDRWKVS *F SPEKSGVMVLSNLIPYTNYSYYVRTMAISSELTNAESDVKNFRTNPGRPSKVTEVVATAISDSKINVTWSYLDKPYGVLT *F RYFIKAKLINRPTRNNNRDYCTEPLVKAMENDLPATTPTKKISDPLAGDCKCVEGSKKTSSQEYDDRKVQAGMEFENALQ *F NFIFVPNIRKSKNGSSDKSDGAEGAALDSNAIPNGGATNPSRRRRDVALEPELDDVEGSVLLRHVRSITDDTDAFFEKDD *F ENTYKDEEDLSSNKQFYEVFAKELPPNQTHFVFEKLRHFTRYAIFVVACREEIPSEKLRDTSFKKSLCSDYDTVFQTTKR *F KKFADIVMDLKVDLEHANNTESPVRVRWTPPVDPNGEIVTYEVAYKLQKPDQVEEKKCIPAADFNQTAGYLIKLNEGLYS *F FRVRANSIAGYGDFTEVEHIKVEPPPSYAKVFFWLLGIGLAFLIVSLFGYVCYLHKRKVPSNDLHMNTEVNPFYASMQYI *F PDDWEVLRENIIQLAPLGQGSFGMVYEGILKSFPPNGVDRECAIKTVNENATDRERTNFLSEASVMKEFDTYHVVRLLGV *F CSRGQPALVVMELMKKGDLKSYLRAHRPEERDEAMMTYLNRIGVTGNVQPPTYGRIYQMAIEIADGMAYLAAKKFVHRDL *F AARNCMVADDLTVKIGDFGMTRDIYETDYYRKGTKGLLPVRWMPPESLRDGVYSSASDVFSFGVVLWEMATLAAQPYQGL *F SNEQVLRYVIDGGVMERPENCPDFLHKLMQRCWHHRSSARPSFLDIIAYLEPQCPNSQFKEVSFYHSEAGLQHREKERKE *F RNQLDAFAAVPLDQDLQDREQQEDATTPLRMGDYQQNSSLDQPPESPIAMVPAIRIHCEQYS *F >pll.AA *F MSGVQTAEAEAQAQNQANGNRTRSRSHLDNTMAIRLLPLPVRAQLCAHLDALDVWQQLATAVKLYPDQVEQISSQKQRGR *F SASNEFLNIWGGQYNHTVQTLFALFKKLKLHNAMRLIKDYVSEDLHKYIPRSVPTISELRAAPDSSAKVNNGPPFPSSSG *F VSNSNNNRTSTTATEEIPSLESLGNIHISTVQRAAESLLEIDYAELENATDGWSPDNRLGQGGFGDVYRGKWKQLDVAIK *F VMNYRSPNIDQKMVELQQSYNELKYLNSIRHDNILALYGYSIKGGKPCLVYQLMKGGSLEARLRAHKAQNPLPALTWQQR *F FSISLGTARGIYFLHTARGTPLIHGDIKPANILLDQCLQPKIGDFGLVREGPKSLDAVVEVNKVFGTKIYLPPEFRNFRQ *F LSTGVDVYSFGIVLLEVFTGRQVTDRVPENETKKNLLDYVKQQWRQNRMELLEKHLAAPMGKELDMCMCAIEAGLHCTAL *F DPQDRPSMNAVLKRFEPFVTD *F >ire-1.AA *F EALMVFSTLGGGLTAIDPVTSEIRWTIADDPPIVAEHQENVQVPHFLPDPRDGSIYQLGQMGSLKKLPYTIPQLVANAPC *F RSSDGILYSGKKSDTWYMVDPKTGRREKVMGFGDATVDGKEGEQIGWATSRAIYLGRTQYTVMMYDSLAKNKDAKPWNIT *F FYDYNAVSAPPELAKEYEYIHLTTTTNGQIVTLDRKHGKFLWQRDLSSPVVAAFLLGPDGLLSVPFTTVSDEAYQAILEE *F SKTGNVNTVKLFQSLYVGEHQKGLYALPSLVDKNTPRISTAPPIKLLDGPTGDQNSNQETDPRTIYINDVLQEHAGIMLG *F HYNMPNEGNGNLQLSPTTASSKVVQSLATIHNYNDGYGLLANNEKNAADIGVQTDPELVEIGIDQRTNGNTINRTKTIIL *F QNSNKVQAFINEWFMEHPSGKVHQILIVIVLGMIALFWYTCSTMRELQKQSENGSKTFAIAQNGSNGSTGSNGSNANAED *F LVDLGNGQVRVGKISFSTNEVLGKGCEGTFVFKGTFEERFVAVKRLLPECFTFADREVALLRESDAHENVVRYFCTEQDR *F QFRYIAVELCAATLQDYTEGDRSLELQNHIDVWQVLSQAASGLSHLHSLDIVHRDIKPQNVLISLPDAKGKVRVMISDFG *F LCKKLNFGKTSFSRRSGVTGTDGWIAPEMMRSQRTTTAVDIFSLGCVYYYVLSGGHHAFGDNLKRQANILSHEYNLAKLR *F PEDDSEDSRIILAEQLISDMIHKDPQSRPPARCIGNHPLFWDEPKMLSFLQDVSDRVEKLQFHAEPLKSLEKNGRIVVLD *F DWNVHLDPMITDDLRKYRGYMGASVRDLLRALRNKKHHYHELTPAAQKMLGCIPHEFTNYWVDRFPQLISHAYHAFSICS *F NEPIFKPYYSAGYLFTRPWYFDADDALFPMLMLDPKPLPKIGSPKKTPSPASSQAQQLKQRKGLYNFRKPNDELPIPGVG *F LQRNLELDGQSLEPDGKRDVFANFKFRRYSKPGNNRNYNGGHKEAQDKEKYVIWTLPPSTQD *F >hop.AA *F MALANGGEDRMDDSSSGRTSLADSASLTNSSLRSGTSSQSIHTNDGTIRVFNFTTGEFERFHPNMLCEEICNTMCRQLGI *F APIAQLLYGIREHSTSRRPSPLVRLDLTWCLPGERLNCQLVYCFRMRFRVPELDSQLELIDGRSHKFLYRQMRYDMRTEQ *F IPEIRYPEHKDKSTGLAVMDMLIDDQEQSEDQQAMRSIEKLYKLYLPPSLWRAHSFFVGSKIREVFRSLKANSLSVERLK *F WHYVHQVSHLAPTYMTEQFTCTVQYLPNEEVARGSGPIGTSLAHSTSTLASSGSTNTLSTLTTNTNSVALGGSGKKAKRR *F STSGGIDVYVRVFPHDSLEPGLKVARVTSEATLKWILVGAVEGIFMISKINDTSVRLEIVGLPKGYEMQFQTEKEMKSFI *F SYLGIYIRLSSKWMQDLCHSYRTPSLEELSSLHCHGPIGGAYSLMKLHENGDKCGSYIVRECDREYNIYYIDINTKIMAK *F KTDQERCKTETFRIVRKDSQWKLSYNNGEHVLNSLHEVAHIIQADSPDRYRIPASKYDKPPLLLLLLPKNLKAKKTDLQL *F SEAELQRRNPQIFNPRTDLQWYPDSISLSDDGMMFTMRGDWIQQSPVKDVSVTMKMLKSDGNFMEFFRLAQTWSLIQSPQ *F FLKLYGLTLADPYTMVMEYSRYGPLNKFLHSMPNVTLHCLLDLMHGLVRGMHYLEDNKIIHNYIRCSNLYVTKYDPNSYV *F LDAKISDPGYPRPYRESDSPWIPVKYYRNLQAAKTDQFAQLWAFATTIYEIFSRCKEDLSTLRQEQLLRQKNLDGNILKM *F LDQDICPAPIFETIMDGWSDDETKRFSHHDIFSRLNTIKAEILPNYMPPPEIATNGTGDETVIDRSDIPFLPFPRSNMLM *F VIPLTSECRVIYNMENMIGRGHYGTVYKGHLEFNDKDQPREQVAIKMLNTMQVSTDFHREIGIMRTLSHPNIVKFKYWAE *F KSHCIIMEYLQSGSFDIYLRFTAPNLNNPRLVSFALDIANGMKYLSDMGLIHRDLAARNILVDHNGDGDCVKISDFGLAQ *F FANSDGYYYAKSKRDIPIRWYSPEAISTCRFSSYSDVWSYGVTLFEMFSRGEEPNLVPIQTSQEDFLNRLQSGERLNRPA *F SCPDFIYDLMQLCWHATPRSRPSFATIVDIITREVATKVTHPTDGHQSPPNQPTDAE *F >cdi.AA *F MSLNQHSGPGPPSEICGLSVDQVDSSAASPFIWPSTAGMCTTATTVTTSTNGDATAEAPVKHQPLHNGGWIGNGLPPAPV *F TRTISSDRLVTGSSCRALRTAVSALYSVDDFVKEKIGSGFFSEVYKVTHRTTGQVMVLKMNQLRANRPNMLREVQLLNKL *F SHANILSFMGVCVQEGQLHALTEYINGGSLEQLLANKEVVLSATQKIRLALGIARGMSYVHDAGIFHRDLTSKNVLIRNL *F ANDQYEAVVGDFGLAAKIPVKSRKSRLETVGSPYWVSPECLKGQWYDQTSDVFSFGIIQCEIIARIEADPDMMPRTASFG *F LDYLAFVELCPMDTPPVFLRLAFYCCLYDAKSRPTFHDATKKLTLLLEKYEHESSAGASPLSSLELINCSPGGSDSENVT *F LTPRASVPTVATICTAPAGTLTPRHLQEVDEDGFRFPSKKSASAALPGTPNGSAGSSGFDDHILKSRVRRATQKLEAEIL *F LHRRSLSENIIHFPMHTSPSDKARCHQMQRQRSSTHSPTPPRQLSSVQLPATSIPDPPPLPKPVPAVLTLRKVGDPQYKP *F TSEKDHNGSKPNPFEALAQLRGVKKILDANPKTYAAGVGDLFSSCFEMLAPFFKELAAMQSKAGAQGGGTSTEETSTGNK *F ASAATPSEPKSLPVSPHMTRKYSAIELRLPQRISNAAPPAVTSAPPPSIALPTDEDDCDDCNSEGEDPDGGGNCADAESS *F SDDSAVRPSTVPTARKYRANSLYAHPLFRGSNGTSMKSTSTGEFESPTLLKASASTITLTASVGATNSCEDLTESARKSK *F EQASSGATAAVQALPTPMSGCSTRLVETPVSVSSCTDLELDELKNTCDLNELKNSCEQLPLKTNLTRRDSIESGFFSCFN *F EESDAAITNNSFGRQLNGIGSLGFGCGGGGNSSNCCYEKLKTQLMLDACVSADSEINDKLASLCRCCYYATSTSNAAVTA *F AAAAAQQQQQSHLLNDTSSTSSSVLLIDNDPAVIGGNTSSSTALDSMDDLDADIVASRRTHQRRYTCHHALLSGNGSESQ *F MAATASLINRLALDSEIHTLLQRSPFATNQLLYCKNRTSSIYTDSSDDISSLAGSDSLLWDDRSFTALPSTRSAQIAKIV *F EYFERKRQDSSPPSVHHRNGNGTVAAAVAASALGSNYLSYESRRYSDFKRHLNADGLCFDLDKKPAQQRMMVCEGAVKSK *F LPIFDKKKQQQGG *F >LIMK1.AA *F MHHQQRLRANGGRGGTGLGAGSGPVSGGHSPLCAHCRGQLLPHPEEPIVMALGQQWHCDCFRCSVCEGHLHNWYFEREGL *F LYCREDYYGRFGDACQQCMAVITGPVMVAGEHKFHPECFCCTACGSFIGEGESYALVERSKLYCGQCYGKRSCQPADAKA *F RITTAGKPMHSIRLVEIPKDATPGLRVDGVALDDGCPTVRITDLFCNFFLWLTSMAEQCCGAPYRIDVNLTNLHIGDRIL *F EVNGTPVSDSSVEQIDKLIRSNEKMLQLTVEHDPVQVCRSCSQADIQRAMSASTLILPLSTSASSVEVGRERLYKTPGEQ *F GTKARKLRQATNASTTIPPAAGATAMTQLKEKERCSSLSKLLDEQHQAQQHSAHPQLYDLSRTQSCRVVQKPQRIFRATD *F LVIGEKLGEGFFGKVFKVTHRQSGEVMVLKELHRADEEAQRNFIKEVAVLRLLDHRHVLKFIGVLYKDKKLHMVTEYVAG *F GCLKELIHDPAQVLPWPQRVRLARDIACGMSYLHSMNIIHRDLNSMNCLVREDRSVIVADFGLARSVDAPRLPSGNMTPG *F GYGSGANSDAPMSPSGTLRRSKSRQRRQRYTVVGNPYWMAPEMMKGLKYDEKLDVFSFGIMLCEIIGRVEADPDFMPRNS *F DFSLNQQEFREKFCAQCPEPFVKVAFVCCDLNPDMRPCFETLHVWLQRLADDLAADRVPPERLLHEIETFQEWYASSEDA *F LSPTSQRSLNNLDELVKSAVDSEISPVEKEKENMVIKPQDIPKSPHLGKDFSPSGERLRDSMRARRRQRFLGAQEERRNL *F TPDTESKERALKKALKKCRPFGERGYLVDLRAGAELQLEDVRDLNTYSDVDSSCDTSLNYHDVNNLPAAQEDENTVKPGK *F EELLEESTNKPSNQESQHHRLAIDDMRTRLNQCRSKFEHLEEASRRNFNQSQHSMKNFFKTPPVALKMFQRLEHEAAALN *F GGNNCPPPPPRTQRINQTPIFGRKNPPVAIVGQKLQHAESLEDLASSGVAKQLATPAPKRSKATATTKGGQSSNPPLFLP *F PSLNISVALNSNGNVTTTTNTNSSCPPSASDWLPKKHKLTLPLPSAQQQRTSSNHRLPMCNNKGKTLKPLPSRTGSQGIP *F ASNCVSPTRSSRPGSPTKHLAQRHTAATAQRLTNAAATHQQQHQQQSSKTTRLNILSPEKVHRLGARLTDQKQKMREEAA *F ATASSVGGAGCAAGTAAGSLNGHRTIGSSGTPNSAVGERRRRAAPSPPVRTHFNTRC *F >CG5483.AA *F MEHPKTGTETALEACDYFVDEVIEASSIRDAREEVRQIKHGELRTAVISGDERTVRVLLAALGTERQIIVNMAPSGANTL *F LFLACQSGYESITQRLLDAGADGRSHAVTKYSPLYAAVHSGHLGIARLMLDHFPELIQQPTVERWLPLHAACINGHIKLL *F ELLISYSYPDYLYQTYRDEEGQWEWRLPFDANAHDVTGQTSLYIASILGNKQLVGVLLKWQLHCRRTLGDSASSVSTPIT *F PTRKRISFGIQAIMSKLHISGESEGPDDLASQESTECQRCPINVNLLCGAARETALLAAVRGGHLDVVQSLLQHGANPNI *F VAKPVEDHNDPKCCEEIYGLSNVPIAEACKQRSLAMLDLLLKHGARDDNGTAIGMAITCGDEAILSRLLARRVHPDSDYK *F INKKGLPTPVEVNVFLPSTSNISYSAMFPNNPTIIDWHSMGSSVQLSVVRVPWMVSGVLLLNPKLQSHPRLNEVALTAIT *F RIDFSHNVLTSIPQELFHLVSLRYLNVAQNKITDLPAPIGQTYGCPVLDELFLQDNQLTTLPAAIFHLPALSILDVSNNK *F LQQLPFDLWRAPKLRELNVAFNLLRDLPVPPMQTSSSLLSLDKLNLQSFEEPPSNKPRNVTQQRLTHRNLWSATLDITDN *F DMKWQHEQDLGDGKTAGVGSSQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEI *F SCWPSLPRITESDPHLLCYSCVQLPEGRDDDYKTASSKGSSSSATSFRASVLKSVCRHRRHLRLEALRTLILADNLLTRI *F QLSTDDATTLFNESEDADWSVVGVNRSKVIFPNLSMLDMTNNCLKEIPASLHELSSLSVLNISGNVNITELPPHLGLLSR *F LWNLNTRGCLLQEPLRSMIESKKHKTMDIVGYLKSIYEDAQPYARMKLMVVGVAGIGKSTLLDLLRQGAGSGSSSSSHRS *F RASENHWAKRMGHARSTSRSHRHSSASSANISTVGVDIGTWICEKRKRAPGSHGPVVFRTWDFGGQKEYYATHQYFLSKR *F SLYLVLWRISDGHKGLAELLQWLGNIQARAPNSPVIIVGTHFDAVGESISPQKAEQLQQLIREKFIAIPDAEKIGLPRVI *F DSIEISCRTLHNIHLLANIIYDTAMQLRSPGSKEPMLLQKIPASYIALEDIVNVIACNLRAAGRDPVLDGEQYKRLVTEQ *F MRLHNYKSFRDAAELQQATTWCHENGVLLHYDDATLRDYYFLDPQWLCDMLAHVVTVREINPFAPTGVMKLDDLQMLFRS *F VQVQGNGNRSQGLRRILLMTYFPSGFWSRLITRILADEQIIEAIRGVYMASQDYADFDLRTSLEQDTQWNLWQTGLALYY *F GPILIFKIWEVPFQKTERTQPFRTDGNRFKLKQDGIWSDVNLSSSSILEVYFPLYEVNISQEVDDNERQLLAEIRPHMSQ *F VAKLLALTVDHIDLLLEDWYPSLGTRFVHTSEGRFLITRLVLCPRCLWKLQLQQNNEPSDREVPPVGCNRPSRSSRRGAG *F AYFLHGVGDPGEDGALNVFSAYLNATARRERRSEDSLGAGSDADSGVGPDSAGSSRNTSVDGHPGYHLPDNSNVCYAWMI *F EECILSVYNQSKISCPVHLEQSMAQLAPDVIFADIPDKHTIPSECIIKGSLLGRGAFGFVFKANCKSALMAFKVAVGKWD *F RDPLQHSCKAYCTARQELAVLLTLKHPNIVPLVGICIKPLALVLELAPLGGLDALLRHYRRSGAHMGPHTFQTLVLQAAR *F AIEYLHRRRIIYRDLKSENVLVWELPQPHTEDSPRNLVHIKIADYGISRQTAPSGAKGFGGTEGFMAPEIIRYNGEEEYT *F EKVDCFSFGMFIYENISLRQPFEGHESIKECILEGSRPALTQRETQFPTCCLDLMVLCWHEQPRRRPTASQIVSILSAPE *F CIHLLDVVAMPHSEKIVCGVFQSLVGMGDDERCGLELWLPSFGSRIDILDCSPSGSLLQCNSISCSPQPQVAPPKTPENG *F ANSRARSAQRLPKMNMLCCCLVGEAIWMGDVSGNLHAYSTSTYAHLFSYMLDPNIKSAVISLVYMEKIARVAVGTHNGRV *F FLVDATQMPSNCAFAEGSFVLTEICSGFVLHAACSVVVDGIYELWCGEIAGKINVFPLNENGVSGHQALCHSEEPNLIED *F VKVARMCSNESHVFSCLYPGCMVYQWDVISKRIENKLDCSKLLPCSESLQSIAIDEHVNLIKCQISALAAHNSELYIGTT *F WGCLIVAELHTLRPISVFRPYENEIKSIITLSKDNVPLIATIGRRYRSLISRYVDSAESSTKSSAVSTPTHGAAKSVPPA *F DVDNHIHCLLWRAKHWT *F >rl.AA *F MEEFNSSGSVVNGTGSTEVPQSNAEVIRGQIFEVGPRYIKLAYIGEGAYGMVVSADDTLTNQRVAIKKISPFEHQTYCQR *F TLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLH *F RDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPI *F FPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEA *F LAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP *F >bsk.AA *F MTTAQHQHYTVEVGDTNFTIHSRYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNH *F KNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKAD *F CTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYTENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLG *F TPSPSFMQRLQPTVRNYVENRPRYTGYSFDRLFPDGLFPNDNNQNSRRKASDARNLLSKMLVIDPEQRISVDEALKHEYI *F NVWYDAEEVDAPAPEPYDHSVDEREHTVEQWKELIYEEVMDYEAHNTNNRTR *F >p38b.AA *F MSRKMAKFYKLDINRTEWEIPETYQNLQPVGQGAYGQVCKAVVRGTSTKVAIKKLARPFQSAVHAKRTYRELRLLKHMDH *F ENVIGLLDVFHPGQPADSLDQFQQVYMVTHLMDADLNNIIRTQKLSDDHVQFLVYQILRGLKYIHSAGVIHRDLKPSNIA *F VNEDCELRILDFGLARPAESEMTGYVATRWYRAPEIMLNWMHYNQTADIWSVGCIMAELLTGRTLFPGTDHIHQLNLIME *F VLGTPADEFMSRISSESARNYIRSLPVMPRRNFRDIFRGANPLAIDLLEKMLELDADKRITAEQALAHPYMEKYHDPTDE *F QTAALYDQSFEENELPVEKWREMVFSEVTAFKPTAAFAELLPKEQ *F >Mpk2.AA *F MSVSITKKFYKLDINRTEWEIPDIYQDLQPVGSGAYGQVSKAVVRGTNMHVAIKKLARPFQSAVHAKRTYRELRLLKHMD *F HENVIGLLDIFHPHPANGSLENFQQVYLVTHLMDADLNNIIRMQHLSDDHVQFLVYQILRGLKYIHSAGVIHRDLKPSNI *F AVNEDCELRILDFGLARPTENEMTGYVATRWYRAPEIMLNWMHYDQTVDIWSVGCIMAELITRRTLFPGTDHIHQLNLIM *F EMLGTPPAEFLKKISSESARSYIQSLPPMKGRSFKNVFKNANPLAIDLLEKMLELDAEKRITAEEALSHPYLEKYAEPSV *F EQTSPPYDHSFEDMDLPVDKWKELIYKEVTNFKPPPSYAQVLKDVK *F >P38c.AA *F MPEFVRVAINESLWEFPDIYEFVRFLGGGSFGQVAKVRLRGTENYFAMKRLMRPFEREEDAKGTYREIRLLKHMNHRNVI *F SLLNVFHPPAHNMMEFQQVYLVTHLMDADLHRYSRSKRMSDQEIRIILYQILRGLKYIHSAGVVHRDLKPCNIAVNGNSE *F VRILDFGLSRMCADKMTDHVGTMWYLAPEIIFLRGQYTKAIDVWSVGCILAELITDRVLFRGENYVSQIRCLINIMGTPT *F REFITGISMERSRNYLEGYPLRQRCDFHHLFMGYDVQAIDLMEKMLEMVPEKRITAAEAMLHPYLRDLIEPHHHAEDTAP *F VYDQNFENMVLPVKCWKELVSHEIRNFRPDQLDLHF *F >CG2309.AA *F MANYQTAHAQERRIQELDQTVESIFDVRKRMGKGAYGIVWKATDRRTKNTVALKKVFDAFRDETDAQRTYREVIFLRAFR *F CHPNIVRLVDIFKASNNLDFYLVFEFMESDLHNVIKRGNVLKDVHKRFVMYQLINAIKFIHSGNVIHRDLKPSNILIDSK *F CRLKVADFGLARTLSSRRIYDDLEQDGMLTDYVATRWYRAPEILVASRNYTKGIDMWGLGCILGEMIRQKPLFQGTSTVN *F QIEKIVTSLPNVTKLDIASIGPSFGSVLLSRNIQRDRRYSLDEMMKNCCDDGISLVKALLVLNPHNRLTAKEAIRHPYVS *F RFQYASAEMDLHMDVVPPLKDHVRYDVDQYRNSLYELIDRETSCSNRTVSNSTPSSNRDELPKPVRVTKQARTTSAKQPT *F TSPAERKKEPSSVDXAAAPPAPAATAPAVPRKSGDKSVPKCQHNNAAVQRELAAVAAAAAVARRKKSSWQSQAQSQGKFH *F TEAKAHVQTAIQTQKDNIKDSPPRMIQESQSLTEAKAPIPKNRYSNKMCQEKKYKKKHHSMSCITRDTFPSETEHRQQRE *F ERAYQRQMKRELQLKESYRRRIEAESEPLKETTEQESKTIKVIEQKVCEHTEKKADESLSKDQQKDSITFGTCVRERIHH *F LELEMEKCTEELVDFVELNADVLNYANVSTHLKKLQRSKESDEKDEDDRRALPEGIGGPGSQNYEIFRQEQEKERQRQVQ *F EFLARDETNEYDNLDLDHAYRAKYYTAYKEIGKELNPAPDSGGRDSGSEHSPGRDNYTTYADYFLKYTTPQQNWNDLERA *F NGLQREHDRIYGLFWLNDRRQEEKYRRKLAQNDQEELPVHHHKACRHRHHKPNHHAPYDHMRPTEDDIQEADSLPESN *F >nmo.AA *F MSVSMSLVQGGAAGGAPQGASAILAAAAPYYQPPAVPQDVQPDRPIGYGAFGVVWAVTDPRDGRRVALKKLPNVFQSLVS *F SKRVFRELKMLCFFKHENVLSALDILQPPHLDFFQEIYVITELLQSDLHKIIVSPQHLSADHIKVFLYQILRGLKYLHSA *F RILHRDIKPGNLLVNSNCVLKICDFGLARVEEPDQAKHMTQEVVTQYYRAPEILMGARHYSSAVDVWSVGCIFGELLGRR *F ILFQAQNPVQQLELITELLGTPTMEDMRHACEGARTHMLRRAPKPPSFSVLYTLSSHATHEAVHLLCQMLVFDPDKRISV *F TDALAHPYLDEGRLRYHSCMCKCCFTTSAGMRQYTADFEPSAGQPFDDLWERKLTSVQQVKEEMHKFIAEQLQTGRVPLC *F INPQSAAFKSFARMRKSTTTTTTTTTTTTCTAAPAAAPIRRRLLLRCPREPIQVQIEILGARSPLTRTTRYRHA *F >Lk6.AA *F MVEPKSGTAASAAAAKASNNNNNNHPRGSGDSGIRSGSGISCSNTDNSCSQSQSDGQNELTRYSSEDVSGNESSEAPNMT *F EVERQAELNRHKEEMQKKRRKKRISSSLHSSTFQELYKLTGEILGEGAYASVQTCVNIYTDLEYAVKVIDKIPGHARARV *F FREVETFHHCQGHLGILQLIEFFEDDEKFYLVFEKINGGPLLSRIQEHICFSEHEASQIIKEIASGLDFLHKKGIAHRDL *F KPENILCVKTDSLCPIKICDFDLGSGIKFTTDISSPAATPQLLTPVGSAEFMAPEVVDLFVGEAHYYDKRCDLWSLGVIA *F YILLCGYPPFSGNCGEDCGWNRGENCRTCQELLFESIQEGHFSFPEAEWHDVSDEAKDLISNLLVKKASNRLSAEAVLNH *F PWIRMCEQEPPASKHGRRHKALQTPSNIRRNHQSAREISQFAESAMAVKRVVLQHFSMRYDYMKERPNIYQPSQAYMDAY *F SDENYNPKPPGHYTRNRSQRNPASSLCGYGGRMSSMHGQRANSRRSSRNASRNASAIYPNSGGFKTLNVHEEDDDDEGLE *F AFGHIDDDDEWSRSRREYQQQCETLGEDRFRRQSGSEGDEVEDDEDGENEDYQHYKHYWRELDEEEGDDYLYEQQQRVDD *F KFGEEEFEDEPKEETQADNLKLSKAYVEQVGETNVEKSKPQDDNGGYIREDLIMDNMDMKKNTQQSEFAKLTIMRNDAQT *F EENKIMQQQDEEKKEEKQQDDVDGAKKQGPSSDISATTITDNNKLQTPVMTTTHINNWQTGDAIEDDDVKLLDSISDLNE *F KLPEIYETANIVVNSAAVPAASTPAASATRPPTDNPEEDDSNVTKPTTTAEGTTMQTTFGMSAEEEKPVALSHTAGHHSK *F TGRTVNFAPDAYQNDEDADIDEDDDYDDEENLHEHSKQQLPSNAYTRKQRQQHQRYIVPRYQLADQVPQRQHTENWRYRT *F HHSQEQQPTADYRKYRPPFSTGGGGGHHGNQQRNYLGSFSHSGGAAGYKIAPMPPPMQPPPRHNSAGSSGSGSGSGGSPP *F SDEQSAIRNWRQDCVYARSCGMNQGPEQQRHNRSSGQRVQQQPRIGSGRFAHLQAAQLMDELPDMRIGLSPPSESVLLQR *F RLRQQQRANDLSEYCEPATASG *F >MAPk-Ak2.AA *F MLSLQNQRQPKTTPLTDDYVTSNTVLGYGINGKVVQCTHRRTQQNYALKVLLDSERARREVDLHWRVSGCRYIVNIIDVY *F ENTFKDRKCLLVVMECMEGGELFQRIQDKADGAFTEREAAQIMHEICAAVDYLHSRDIAHRDLKPENLLYTTTQPNATLK *F LTDFGFAKETFTSYTLQTPCYTPYYVAPEVLGPEKYDKSCDIWSLGVVMYIIMCGFPPFYSNHGLAISPGMKNRIRTGQY *F DFPDPEWTNVSQAAKDLIKGMLNVDPSKRLRIQDVISNKWIAQYNAVPQTPLCTGRMLKEAEETWPEVQEEMTRSLATMR *F VDYDQMQIKALDKSNNPLLTKRRKKIEEMELYMANATRN *F >gwl.AA *F MENADATSQSDVHIDYKTPKKTHSLIDSEQLLDKINILTTKPENHSQNAKLPTIKDFVIIKPISRGAFGKVFLGYKNNDS *F KRLFAIKVMRKSEMINKNMVSQVITERNALALSRSQFCVSLFYSLQSLSYVYLVMEYMVGGDLKSLLAMFGYFDEPTARF *F YVAEMVMALQYLHQHGIVHRDIKPDNMLLSSSGHVKLTDFGLSKIDMRRDLEISDLINCSPNLNARTPGQLLSLTSHLSF *F GSEKKLNDFGSVSSGQNNGMGSVATGTSHLLQAINKHSLIMELSDSEGDTSLNDAEKTSDSKISGVSPFFSAEEANESIT *F HTCTTNVNVGKHIAIKMDLFIYHSPNLQPQDSSSSCSFHTCNSADLSKCSPPLESKDGAAAGNAIPSKRRVEFVLDAAPC *F QGCKLAEQDSSNMATNDGKHLPKIDNAIEASFEFSMVRRRSVDERNRISKGPEDSGVSSRKGDDYSSCHLNLNSESTASS *F IEKNVDNLSQSKEDFSCSDYSRSYNVTNGNEMSGINMNSPFRNLSKHFKRPDFLRGMKRKINLVNRSDNMSSMDTDGCSS *F SNGSTNTGLTQEIEILNIGSSTPKKRKARSSPIRGVLKVRSLSDDEMPINHLLGPEANVANVVFSTPVSSQKLPRRDGGL *F LGKLKATRFALPLSIENKKREHATADKMSGIQYHLKLSDDPTMSPINHGAGNLPKTPKNVNINTPFRTPKSVRRGARVSN *F ERILGTPDYLAPELLLKQGHGPAVDWWALGVCFYEFMTGIPPFNDETPQKVFDNILNKNIEWPEGDEALSVESMEAVELL *F LTMDPNERPAAKEVQQMRHFACIDWENIGNTEPPFVPTPDNPTDTGYFDARNNLQHLQLSNFALEE *F >CG6498.AA *F MSRQEGAASRPADGATGPVASSSSSTSSNHSVSAVLGSKISTSTPQKNDEHQEQEFINQSVASEIEAMSISDSASAESSS *F GATATATATMSGSPVPKRHLSTANLSRIRPQSSYSARVLIFDDSDQEAAAAAAALAAASSSCNSSVKSCSGLELLPTSPA *F KLSIGNDSTNGSSMMRNLNLTKSSSGGLSGSSSSLHSRGYTALLRKISYQQHTNSLRAVSGETSNLLRMRHSSLGKSAPC *F LTGNYFRHELTAPLPVQPPGFGASPLGGHNISRSGSCAGIGLAKHHHHHHLHGVVMRGSAGGGAGSGGGSSSGVAHHRLS *F LVTNSAAVAAAGGSRTHSPYSASPVDSPRLNSPMPFAFAPIKRIASCRGVVADGRRWSVASLPSSGYGTTPGSSNLSSQC *F SSQEGLNQLAHNIPPGVQEADAAAVAAGACCAEHLKQHCPKHCALLLAVSQKQLTPQPPKPTQLQPQKSMTAACVNCCAE *F LSVTCGGQQAGGGAANGGSSANSSGSSAMQVGGRMSPYFRPRSRSLSSPSRSPIVDNEIAVMNTLYKERFPKATQQMEER *F LKLFINENKSAACNSFRDSQPIVRFVHHQVLEMARDCLHKSEAKLITSQYFYELSENLERLLVETKEKSPEAAAELNGVI *F KKLLLIISRPARLLECLEFDPQEFYELLEAAEGHAKAMPVIKADIPQYIIHKLGLNRDPIAELQQELRETQQMCSEQVTV *F DADPLHPGGSLLLNSPLTSAAKQLSSLALDAIAMDSGSGTATPQQPPQTPVATCDTAPSFALAISQMNEEKGGSSSAVGG *F GSSSKVAGAEGITGGSAATATGSGAQQPSPQENDFDIVKLISNGAYGAVYLVKHKTTRQRFAMKKINKNNLILRNQVEQV *F FAERDILSFADNPFVVSMYCSFETKKHLCLVMEYVEGGDCGTLLKNIGPLPADMARFYFAETVLAVEYLHSYGIVHRDLK *F PDNLLITALGHIKLTDFGLSKMGLMSLATNLYEGYIDSETRQFSDKQVYGTPEYIAPEVILRQGYGKPVDWWSMGIILYE *F FLIGCVPFFGETTEELFAHTVNDDIEWPDSEDWPVQAEAKDIISQLLQQNPRDRLGTQSGALEMKEHEYFLGMDWNSLLR *F QKAEFVPQLSHDDDTSYFDTRMDRYNHDLGGEDTDDTDDTPVFGSFNSYTPQYRKQHYSWSRHATPTSTDGAKPTGPPPL *F SSLPSRAQETPAATVGATSTGALPKLKSGMGSGSGSGSGSGSGSASHDGVVNKFLNTPQLRKLDLSSSCLKVPSTPDADY *F LPELLHNVTIGNDAELRMLKHYLQQPNPGATQRMQQRHSMPPNTTTIISTPATPPPTQTQATMAATPPTATVGSFSRSTP *F ESSQTDSDDFSPQINRKRKGVCARDILPRFSISIEDETISAGSSSTENMNLPREQSPLALQHQPKSMDGSTSGSSVKHHR *F SRSIVKSASALGLSLMTSLDNSQLAAQLCGIQSPGGGGNGSSTASSRDTSPCRELSPLVTNLKPPIIIRRGPRGFGFTVH *F TIRVYYGDTDFYTMHHLVMAVDEGSPAFEAGLRPADLITHVNGEAVQGLFHTQVLQLLLSGGEHVTLRATPLEHTSIQSG *F GRKRDLMQSKLAKKGVNRQKKQTKREHDKKRKTSLFRRISSKRANAEMQQYSAGTSSPTTPSARNLSPLDSSYHSSCCQS *F AANSSSQSTSPSSSSPNTPTGSSGSNHGGNAGSVAVAPSALSVQLPSAPPPTQLVLLPHVGAVVGSNPTSVGNVPVSPTG *F VVPQLYQRPSTLHGLKHKLHAATAVIAGGGNANNPAGGLKTLHTTNNNSLPNRRKSVGHIPLSPLARTPSPSPLPSSPTR *F SPSPLAFPLVGHQPGASNTTQSYSPGSTLPTLQTAVNANTKKAGFARTKSAEPSSPLLRRALSPDRLHPRSAETKISPLC *F CSPPIKQPTHQRVVTTTWRSTPGGSASGAGGAVLGAPSVQPLQLVPAASEESQRQTAMATVVSTTAAGTDPSATAVVTLA *F NCEPLPRIAEEKDSPTSTQDSSSVSEGFMPAIEEFAEGESSSSPTQTLEKPTKVKATEQPEMEPAGKSKKVDQGKTTTPG *F TPKTKPHNSGCKTSMTTTKPASPSVTISTAPRKDTSAVTGGGSSGSGGSAATSAAKQRK *F >CG1776.AA *F MIYIDDSEPEGVLEPAFPMRDVTINRNVDAHKHYDVLGEVGRGKFGTVYKCRDKANGLQLAAKFVPIPKREDKRNVEREV *F EIMNSLQHHLIIQLYAAYEYQKMMCVVLELIEGGELFDRVVDDEFVLTERVCRVFIRQVCEAMAFIHGNGIVHLDLKPEN *F ILVLTQKGNRIKIIDFGLARKFDPDKRLRVLFGTPEFVAPEVVNFDCISYGTDMWSVGVICYVLISGLSPFMGENDIETM *F SNVTIAKYDFEDECFNGISPECLDFIAKLLAKDLSTRMTAAECMKHKWLQQRPATAATATPITKAASAASKSRLKSVSPV *F TAPSESSEDSTETIEDEDDEEEVAVQQAKQKDQQQDEELANLCGDAELENKELDATKDNLKNFIVRWETHPNSPYVFDVE *F GNVIAPLSETSYPHPRRTHGADSLSSSRVCSPSPCDSISTLTDDERGDIEDLPEEDESRSAENESPRSVATPINESREKL *F FPTVATSSPSTPTPQHLFNENFDEFSGSQSTAQQQRSMKSYLHTFDRRNSDTTYLLGRRSSGERVNLADEIRKLSDHLLM *F LAEINTKLGDANNNGSSTSVAPSGSTSSKTSEISQEGNRWTSKTTSSSSWNRPTLKGGLFSQASSSSQSQSTYDDGKGKT *F KISSLSVRLQQSIEETPKLSNGNSSSSKSLVQSQRKSTVQTMSSSNARSFTNQQVQRTSTTSSKVISSSTRSSNTSSSNY *F IASNASNSISTSASNPSSTGASNPITTSASNESASSRRAKFRINQMSRDVPVGLPDTHQTVNLEEAANTTKDCLLHLLEK *F YNETRIRNPVGRHQSISVDWRVSDNLEYRSMSSINAFFQRHNPNGGGHNVRHIQAQLEEKAAGK *F >CG18021.AA *F MDEEIDDERTRSRISMYAANESYSIRRLRTELGPRLDEYTEADAMIETQREGYPPFFREKPQTIAITENQPSHIHCFAVG *F DPKPCVQWFKNDMVLTESKRIKISVDEDGRSILRFEPALHFDVGVYKVVARNKVGQTVARCRIVVATLPDAPDSPEISAN *F SGTEILLRWKQPRDDGHSTVLCYSLQYKLSNCDAWTTVADNIDHEFYLLHDLQPNTNYQFRLASKNRIGWSEMGIPVSAS *F TVGGDAPKIHITKAMKHLQQLTENGHQVVPEEERVHTDYHCEREPPNWVTDSSVSDKYSFISEIARGEFSTIVKGIQKST *F DTVVVAKILEVTDENEDNVVAEFDNFKTLRHERIPALFSAYKPLNVPIAIFVMEKLQGADVLTYFSSRHEYSEQMVATVV *F TQLLDALQYLHWRGYCHLNIQPDNVVMASVRSIQVKLVDFGSAKKVNKLGMKVTPCGSLDFQPPEMINDEPIFPQSDIWS *F LGALTYLLLSGCSPFRGADEYETKQNISFVRYRFENLFKEVTPEATRFIMLLFKRHPTKRPYTEDCLEHRWLMSSDYMVR *F KRERAIFLGSRLKTFCDEYHDLKNASATSSKVLNTVAGGPTPTQLLRSNSIQEELLTTF *F >bt.AA *F MGEVQWLRNGEEIKPDKRIQIVKDGRKRKLVIKDCKVTDAGQFKCTTNADTTESEIIINYQNRFNKKLKDTEAVEREKLI *F LDIELQDQTAPCDWKFNGEPIVPSESIEIKNMGGGKHQLIFSSLDMSNEGEITCESGQLSSKCKLSIRKGESRPNIDCPD *F KFSGPISAPVLLEVPFKVSGTKQTPVEAKLFKDGKPLPVKDVEVAVTDDKVTFKIKKPSRDLSGPYQIKISNGQGEDTKD *F VQIICQDVPQPPQDVDITDVYQTSCVVSFNPPSDDGGTPITKYVIERQDLSKKHGWESVAEVLPSEPCLKKIDDLIPKKQ *F YRFRIRAVNAIGQSDPATFKNTILAKDPWDEPGKPKAVDLTDWDKDHADLKWEAPETDGGDPITAYIVEYKEKFSNDWVS *F GKEVDGDARTATVDGLKEGQQYEFRVRAVNRAGPGEPSDKTKSIIAKCRFVKPFIVGEGLKNVTVKKGQTIRFDIKYDGE *F PEPAATWVKGTDNLKFDNQRICLDQLERNSSITIKKSVRKDTGKYKLVLSNSSGTIESEAQVVVLDRPLPPGGPFEPEEI *F RASHIKMKWKRPDDDGGCEISGYALERMDEETGRWIPAGEVGPNETSFDFKGLTPNKKYKFRVKAINKEGESEPLETFDA *F IVARNPYDPPSPPSQPVIDDYDNKSVLLKWKRPPSDGGRPITHYIVEIKDKFAPSWSEVAKTDDPNPECNVEGLKEKMVY *F QFRVRAVNKAGPSEPSQPTDNHLCKHKNLKPQIDRSTFKRVTIKSGRTHKWSVDVLGEPIPELHWSWRDDIPLTNGDRIK *F IENVDYHTDFSITNVLRKDSGFYTLKAENRNGIDRETVELVVLGKPSSPKGPLAVSDVTASGCKLQWKKPEDDGGVPIKE *F YVVEKMDTATGKWVRVGRSPGEKEPPSFDVTGLSLGSEYMFRVSAVNEEGESEPLTTLVGVVAKDPFDEPNKPGTPEVTD *F YDNQSISLKWAAPNNDGGAPIQKYIIEKKNKNKTEWEKALEIPGDQLEATVAGLQEYGEYQFRVIAVNKAGLSPPSDASV *F PQIVKYKKLKPRIDRSNLKPLLIRAGKPIRYDVNVRGEPAPVITWYQNDKELKPEELPSSSEIKNIPYNTKISIIETVRK *F HTGIYKIIAVNEHGQDEATVEVNILAPPSKPRGPLDVKDVTKDSCKLKWKKPEDDGGKPISAYQVEKFDKKQGRWVPLGR *F TSANDTEFDVKGLQEGHEYQFRVKAINEEGESDPLDSDDSIIAKNPYDAASKPGTPNIVDYNEHMVKLKWEAPRSDGGAP *F ISGYIIEKKDKFSPIWDEILSTNTSVPEATVEGLVEGNIYQFRVRAVNKAGFSDPSDATEPHLAKPRNLKPYINRDKMKP *F IKVRAGQPVKFDVDVKGEPAPSLTWFLKETELTSTGQVRLENIDYNTKLTLLDTDRKQSGQYKLRAENINGVDEAVVEVI *F ILDKPSKPEGPIEVSDIHKEGCKLKWRKPKDDGGIPITGYVIEKMDTATGKWVPAGSVDPEKYDIEIKGLDPNHRYQFRV *F KAVNEEGESEPLETESAITAKNPFDVSAPPGLPELEDWDEHHVKLKWEPPIRDGGSPITNYIIEVMDKDSGEFVKAVETD *F SPVCKGVVKKLEEGQQYKFRVRAVNKAGPSDPSEQTNWHVAKPRFLKPHIDRVNLKPVIVKTGLSISLDINIRGEPAPKV *F EWFFNNSSVTSDEHSVKIDNVDYNTKFFVMRAQRSQSGKYIIKATNEVGEDEAELEVTVLGKPGKPKGPLQVNDITKHSC *F KLKWEKPDDDGGSPIDYYEIEKLDPHTGQWLPCGKSTEPEAKVIGLHEGKAYKFRVRAVNKEGESEDLETEKPIIAKNPY *F DEPDRPGKPEPTNWDKDFVDLAWDPPKNDGGAPIQKYVIQMRDKSGRAWVDSATVPGDKCNGTVTGVEEGHEYEFRIVAV *F NKAGPSDPSDVSKSVIAKPRFLKPHIDRKNLQKKIMRSGQMLHIDALIKAEPPAKVTWTYNKTEIKTSDHIKIENEDYKT *F TFIMPKVKRADRGIYIVTAKNDSGSDTVEVELEVLCKPSKPKGPLAVSNVTAETLHLKWEKPEDDGGDPIEQYLVERMDT *F ETGRWVPVLTTKTPEADVTGLTEGKEYLFRVKAVNSEGESEPLVTDIPTKAKNPFDAADTPGKPQIVDWSGNHCDLKWRA *F PEDDGGASITGYIVERKDPNTGKWQKALETSTPDCKARVNDLIAGNKYQFRIMAVNKAGKSKPSEPSDQMTAKDRFAPPK *F IDRTNIKDITIKAGQHIRFDIKVSGEPPATKVWLHNKARLENDDSNYNIDMESYRTKLTVPISKRFHSGKYTLKAENESG *F RDEASFEVIVLDKPGPPEGPLRVTDVHKEGCKLKWNAPLDDGGLPIDHYIIEKMDVESGRWLPSGRFKESFAELNNLEPS *F HEYKFRVLAVNTEGESEPLTGEQSVIAKNPFDEPGKPGTPEAVDWDKDHVDLVWRPPINDGGSPITGYVVEKREKGTDKW *F IKGTEITIPCLGEECKATVPTLNENCEYEFRVKAINAAGPGEPSDASKPIITKPRKLAPKIDRKNIRTYNFKSGEPIFLD *F INISGEPAPDVTWNQNNKSVQTTSFSHIENLPYNTKYINNNPERKDTGLYKISAHNFYGQDQVEFQINIITKPGKPEGPL *F EVSEVHKDGCKLKWKKPKDDGGEPVESYLVEKFDPDTGIWLPVGRSDGPEYNVDGLVPGHDYKFRVKAVNKEGESEPLET *F LGSIIAKDPFSVPTKPGVPEPTDWTANKVELAWPEPASDGGSPIQGYIVEVKDKYSPLWEKALETNSPTPTATVQGLIEG *F NEYQFRVVALNKGGLSEPSDPSKIFTAKPRYLAPKIDRRNLRNITLSSGTALKLDANITGEPAPKVEWKLSNYHLQSGKN *F VTIETPDYYTKLVIRPTQRSDSGEYLVTATNTSGKDSVLVNVVITDKPSPPNGPLQISDVHKEGCHLKWKRPSDDGGTPI *F EYFQIDKLEPETGCWIPSCRSTEPQVDVTGLSPGNEYKFRVSAVNAEGESQPLVGDESIVARNPFDEPGKPENLKATDWD *F KDHVDLAWTPPLIDGGSPISCYIIEKQDKYGKWERALDVPADQCKATIPDLVEGQTYKFRVSAVNAAGTGEPSDSTPPII *F AKARNKPPIIDRSSLVEVRIKAGQSFTFDCKVSGEPAPQTKWLLKKKEVYSKDNVKVTNVDYNTKLKVNSATRSDSGIYT *F VFAENANGEDSADVKVTVIDKPAPPNGPLKVDEINSESCTLHWNPPDDDGGQPIDNYVVEKLDETTGRWIPAGETDGPVT *F ALKVGGLTPGHKYKFRVRAKNRQGTSEPLTTAQAIIAKNPFDVPTKPGTPTIKDFDKEFVDLEWTRPEADGGSPITGYVV *F EKRDKFSPDWEKCAEISDDITNAHVPDLIEGLKYEFRVRAVNKAGPGSPSDATETHVARPKNTPPKIDRNFMSDIKIKAG *F NVFEFDVPVTGEPLPSKDWTHEGNMIINTDRVKISNFDDRTKIRILDAKRSDTGVYTLTARNINGTDRHNVKVTILDAPS *F VPEGPLRNGDVSKNSIVLRWRPPKDDGGSEITHYVVEKMDNEAMRWVPVGDCTDTEIRADNLIENHDYSFRVRAVNKQGQ *F SQPLTTSQPITAKDPYSHPDKPGQPQATDWGKHFVDLEWSTPKRDGGAPISSYIIEKRPKFGQWERAAVVLGDNCKAHVP *F ELTNGGEYEFRVIAVNRGGPSDPSDPSSTIICKPRFLAPFFDKSLLNDITVHAGKRLGWTLPIEASPRPLITWLYNGKEI *F GSNSRGESGLFQNELTFEIVSSLRSDEGRYTLILKNEHGSFDASAHATVLDRPSPPKGPLDITKITRDGCHLTWNVPDDD *F GGSPILHYIIEKMDLSRSTWSDAGMSTHIVHDVTRLVHRKEYLFRVKAVNAIGESDPLEAVNTIIAKNEFDEPDAPGKPI *F ITDWDRDHIDLQWAVPKSDGGAPISEYIIQKKEKGSPYWTNVRHVPSNKNTTTIPELTEGQEYEFRVIAVNQAGQSEPSE *F PSDMIMAKPRYLPPKIITPLNEVRIKCGLIFHTDIHFIGEPAPEATWTLNSNPLLSNDRSTITSIGHHSVVHTVNCQRSD *F SGIYHLLLRNSSGIDEGSFELVVLDRPGPPEGPMEYEEITANSVTISWKPPKDNGGSEISSYVIEKRDLTHGGGWVPAVN *F YVSAKYNHAVVPRLLEGTMYELRVMAENLQGRSDPLTSDQPVVAKSQYTVPGAPGKPELTDSDKNHITIKWKQPISNGGS *F PIIGYDIERRDVNTGRWIKINGQPVPTAEYQDDRVTSNHQYQYRISAVNAAGNGKTSEPSAIFNARPLREKPRFYFDGLI *F GKRIKVRAGEPVNLNIPISGAPTPTIEWKRGDLKLEEGKRISYETNSERTLFRIDDSNRRDSGKYTVTAANEFGKDTADI *F EVIVVDKPSPPEGPLSYTETAPDHISLHWYSPKDDGGSDITGYIIEFTEFGVDDWKPVPGTCPNTNFTVKNLVEGKKYVF *F RIRAENIYGASEALEGKPVLAKSPFDPPGAPSQPTISAYTPNSANLEWHPPDDCGGKPITGYIVERRERGGEWIKCNNYP *F TPNTSYTVSNLRDGARYEFRVLAVNEAGPGHPSKPSDPMTAEHQRYRPDPPEPPKPDRITRNGVTLSWRPPRTDGKSRIK *F GYYVEMRPKNGKDWKTVNDIPINSTVYTVPSLKEGEEYSFRVVAENEVGRSDPSKPSQPITIEEQPNKPCMELGKVRDIV *F CRAGDDFSIHVPYLAFPKPNAFWYSNDNMLDDNNRVHKHLTDDAASVVVKNSKRADSGQYRLQLKNTSGFDTATINVRVL *F DRPSPPTRLRADEFSGDSLTLYWNPPNDDGGSAIQNYIIEKKEARSSTWSKVSSFCTVPFVRIRNLVLNKEYDFRVIAEN *F KYGQSDPANTSEPILARHPFDIPNTPGIPHGIDSTEDSITIAWTKPKHDGGSPITGYIIEKRLLSDDKWTKAVHALCPDL *F SCKIPNLIENAEYEFRVAAVNAAGQSAYSGSSDLIFCRRPPHAPKITSDLSIRDMTVIAGDEFRITVPYHASPRPTASWS *F LNGLEVIPGERIKFDSNDYASMYYNKSAKRDETGSYTITLTNNKGSDTASCHVTVVDRPLPPQGPLNAYDITPDTCTLAW *F KTPLDDGGSPITNYVVEKLDNSGSWVKISSFVRNTHYDVMGLEPHYKYNFRVRAENQYGLSDPLDIIEPIVAKHQFTVPD *F EPGQPKVIDWDSGNVTLIWTRPLSDGGSRIQGYQIEYRDILNDSSWNAYDYIIKDTKYQLYNLINGSEYEFRIKAKNAAG *F LSKPSSPSLRFKLKGKFTVPSPPGAPQVTRVGKNYVDLKWEKPLRDGGSRITGYIIERRDIGGAVWVKCNDYNVLDTEYT *F VMNLIEMGDYEFRVFAVNSAGRSEPSLCTMPIKVCEVLGGKKPDWITRLQDKVAPFGKDYTLQCAASGKPSPTARWLRNG *F KEIQMNGGRMTCDSKDGVFRLHISNVQTGDDGDYTCEAMNSLGFVNTSGYLKIGSPPIINRCPSELKLPEGDNSKIKIFY *F SGDQPLTVILKKNNEVICDSNDDTHVKVNIFDDYVAIYIRNIVKSDGGPYQIEFTNESGSATGEFYVHITGMPSAPTGPM *F GISYINKNSCMLNWRPPSYDGGLKVSHYVIERKDVSSPHWITVSSTCKDTAFNVQGLIENQEYIFRVMAVNENGMGPPLE *F GLNPIRAKDPIDPPSPPGSPQITEIGGDFVHLEWEKPESDGGAHIQGYWIDKREVGSNTWQRVNATICAANQINCINLIE *F GRQYEFRIFAQNVAGLSTESSASQAVKIIDPQAASPPLIVKPLRDANCIQNHNAQFTCTINGVPKPTISWYKGAREISNG *F ARYHMYSEGDNHFLNINDVFGEDADEYVCRAVNKAGAKSTRATLAIMTAPKLNVPPRFRDTAYFDKGENVVIKIPFTGFP *F KPRIHWVRDGENIESGGHYTVEVKERHAVLIIRDGSHLDSGPYRITAENELGSDTAIIQVQISDRPDPPRFPLIESIGTE *F SLSLSWKAPVWDGCSDITNYYVERREHPLSSWIRVGNTRFTSMAVSGLTPGKEYDFRIFADNVYGRSDASDTSTLIKTKE *F SVKKKPIERKWEIDANGRKLRGKADGPVKDYDSYVFDIYSKFVPQPVEISQQSVYDRYDILEEIGTGAFGVVHRCRERST *F GNIFAAKFIPVSHSVEKDLIRREIDIMNQLHHQKLINLHDAFEDDDEMILILEFLSGGELFERITAEGYVMTEAEVINYM *F RQICEGIRHMHEQNIIHLDIKPENIMCQTRSSTNVKLIDFGLATRLDPNEVVKITTGTAEFAAPEIVNREPVGFYTDMWA *F TGVLSYVLLSGLSPFAGDNDVQTLKNVKACDWDFDVESFKYISEEAKDFIRKLLVRNKEKRMTAHECLLHPWLTGDHSAM *F KQEINRDRYLAYREKLRRKYEDFERFLLPIGRLSEYSSLRKLLMEKYKIHDAVFDRRQAAPRFVIRPSSQFCYEGQSVKF *F YCRCIAIATPTLTWSHNNIELRQSVKFMKRYVGDDYYFIINRVKLDDRGEYIIRAENHYGSREEVVFLNVQPLPKEQPRY *F RTESTPVRRREPLPYTFWQEESETAPSFTFLLRPRVMQARDTCKLLCCLSGKPVPNVRWYKDGRELSKYEYAMTHSDGVV *F TMEIIDCKPSDSGKYSCKATNCHGTDETDCVVIVEGEWVTPEQAQLAHNFLYSGDRKYIEQPIKPAPLPIVTSRQYTSSS *F VQNTSEPQGDKVNVSNSNSSGISNKKKYASNSLQAPGSPSRSRSATKELILPPDDSLMCKPEFTKPLHDLTIHDGEQLIL *F TCYVKGDPEPQISWSKNGKSLSSSDILDLRYKNGIATLTINEVFPEDEGVITCTATNSVGAVETKCKLTIQPLDKNINKR *F KVNAGDNAPKIVSHLESRFVRDGDAVNLACRIIGAQHFDVVWLHNNKEIKPSKDFQYTNEANIYRLQIAEIFPEDGGTYT *F CEAFNDIGESFSTCTINVTVPGDETKQPSFVKFPTSVSVLEGEGTTFECEIDSELLNLVWLKDGKPIDETLPRYSFTKDG *F HRYSFAVAKCNMDDVGQYQAKAVSGKAESICKSLAIIVHNFKFQKSLCEIFPAATLNFMIRKQSQWLFVFFGCIDNTWGP *F SCRKDLETLTDISLRVLFRGWLETHTDNGGKLFTLSADPPSLVAYKAYLYDGDRC *F >CG18020.AA *F MWFVYGYPKPKMTYYFDDMLIESGGRFDQSYTRNGQATLFINKMLDRDVGWYEAVATNEHGEARQRVRLEIAEHPRFLKR *F PDETFIMARKNGRIEAKLVGIPLPEVHWFKDWKPIVDSSRIKISSYDPDIYVLSIHDSIIKDGGLYSISARNIAGSISTS *F VTVHIEENEDQYIYKTYGRHPYVRSKQLRYQDKYDIGDELGRGTQGITYHAVERSSGDNYAAKIMYGRPELRPFMLNELE *F MMNTFNHKNLIRPYDAYDTDRSVTLIMELAAGGELVRDNLLRRDYYTERDIAHYIRQTLWGLEHMHEMGVGHMGLTIKDL *F LISVVGGDIIKVSDFGLSRKINRHNLSTLDYGMPEFVSPEVVNKEGVNFSHDMWTVGLITYVLLGGHNPFLGIDDRETLT *F KIREGRWDFKDEIWTHISDDGRDFISRLLLYSPEERMDVKTALKHPWFFMLDRPVYDHDYQIGTDRLRNYYDHFRDWYAN *F ASCKNYFRRRRLSGCFQHPSKMVYPPGHVYTPENTPEPLPEPRIRAKREEVVSKYLHPDYELGLIQSESQ *F >Strn-Mlck.AA *F MRDVTASRSGEICLQVKHPQAEFRRIPTTRTYTSLLVLPAIRGNSSSSSLAARSCILTRPEDCTALIGGHVRLSVRYEPF *F PGTKVIWYKACHPIVESSNVTIRTTSQQSTLYITDISADDSGKYTVEVMNDYGVEAAAASVAVEGPPEPPSGQPSVSLGP *F DRVAVAWCGPPYDGGCMITGFIIEMQTIGDENCDEDSWQQVTRVVDSLAYTVKNLQPERQYRFRVRAENIHGRSAPGQAS *F ELVQITNTPQRSTSSDASDRFGQATVSVQSGGDFKSRFEIIEELGKGRFGIVYKVQERGQPEQLLAAKVIKCIKSQDRQK *F VLEEISIMRALQHPKLLQLAASFESPREIVMVMEYITGGELFERVVADDFTLTEMDCILFLRQVCDGVAYMHGQSVVHLD *F LKPENIMCHTRTSHQIKIIDFGLAQRLDTKAPVRVLFGTPEFIPPEIISYEPIGFQSDMWSVGVICYVLLSGLSPFMGDT *F DVETFSNITRADYDYDDEAFDCVSQEAKDFISQLLVHRKEDRLTAQQCLASKWLSQRPDDSLSNNKICTDKLKKFIIRRK *F WQKTGNAIRALGRMANLSVSRRNSAIAMGVLSSPRPSISGLGMLTTSAIGSGTSSQMTSLHEEEDDFSGEMPPVEKRTVL *F KLRDKSQCSERSDSGYSECSNCSGAQETLLLSLAKSKLEAIAKASTLPTVVHDTEQPVSLELPTKGEAIMRSDFTNTIKM *F RKKSLEDSAAREKPRSKPQVKPLLCESKLKVSQLKDRFQVSPAPASASASASAANKPPLAYGPFKIAKVASVGRISRTEE *F PGRSGRGAPSVSGKGKSPQVRSMPSSPLPQRSATPTRLMSQRVREAAERLAQQHTVASAQRHLGNGRGTGTGNGNGNSNS *F NGNGNGNTAETNRESRARRLINRFNSETQHITS *F >Tak1.AA *F MATASLDALQAAYVDFSEITLREKVGHGSYGVVCKAVWRDKLVAVKEFFASAEQKDIEKEVKQLSRVKHPNIIALHGISS *F YQQATYLIMEFAEGGSLHNFLHGKVKPAYSLAHAMSWARQCAEGLAYLHAMTPKPLIHRDVKPLNLLLTNKGRNLKICDF *F GTVADKSTMMTNNRGSAAWMAPEVFEGSKYTEKCDIFSWAIVLWEVLSRKQPFKGIDNAYTIQWKIYKGERPPLLTTCPK *F RIEDLMTACWKTVPEDRPSMQYIVGVMHEIVKDYTGADKALEYTFVNQQIVTKESDGTVAAQPDSLSSQEGELSPSSTQL *F TPTTAANANVNAIAISKTTTSSMTENTSSTSSDITPTNSGQLDNNPLFYMVTNRWDAIPEEESNESRNDSFNLTSSAEAT *F QRLETIRNGMILMACKPMEQLTLDVEANGFDLSPSESSSSSTNAKSDGRERLTVTDTKPVMMTTDLSNNNGGIHAHSNGL *F LSHANGWQARDEELQEQEHEQEIVNSLDVDVDPDEDENDGTEQSLAEILDPELQPEPPIPNDAESQLIYRDHRHMAKEYL *F SVDTNLYYAQDFKDKLIVQMDRTEREQKQELLRKMKDKEGLQSLYNNLQQQYASRQLAAGHHPQPHPHPHPNQLQHPHSH *F PPMHFLQDEGCGLLPGSVCGGSESVEEGWVVIPPHHNA *F >Takl1.AA *F MVKQVDFAEVKLSEKFLGAGSGGAVRKATFQNQEIAVKIFDFLEETIKKNAEREITHLSEIDHENVIRVIGRASNGKKDY *F LLMEYLEEGSLHNYLYGDDKWEYTVEQAVRWALQCAKALAYLHSLDRPIVHRDIKPQNMLLYNQHEDLKICDFGLATDMS *F NNKTDMQGTLRYMAPEAIKHLKYTAKCDVYSFGIMLWELMTRQLPYSHLENPNSQYAIMKAISSGEKLPMEAVRSDCPEG *F IKQLMECCMDINPEKRPSMKEIEKFLGEQYESGTDEDFIKPLDEDTVAVVTYHVDSSGSRIMRVDFWRHQLPSIRMTFPI *F VKREAERLGKTVVREMAKAAADGDREVRRAEKDTERETSRAAHNGERETRRAGQDVGRETVRAVKKIGKKLRF *F >slpr.AA *F MLPISEEQQLQQQQQQQQLEQLHHPQIPEIPIPDLEQVETQVGDGSLWTALYDYDAQGEDELTLRRGEIVVVLSTDSEVS *F GDVGWWTGKIGDKVGVFPKDFVTDEDPLQLNVSSAIGDIQPHEIEYNELDIKEVIGSGGFCKVHRGYYDGEEVAIKIAHQ *F TGEDDMQRMRDNVLQEAKLFWALKHENIAALRGVCLNTKLCLVMEYARGGSLNRILAGKIPPDVLVNWAIQIARGMNYLH *F NEAPMSIIHRDLKSSNVLIYEAIEGNHLQQKTLKITDFGLAREMYNTQRMSAAGTYAWMPPEVISVSTYSKFSDVWSYGV *F LLWELITGETPYKGFDPLSVAYGVAVNTLTLPIPKTCPETWGALMKSCWQTDPHKRPGFKEILKQLESIACSKFTLTPQE *F SFHYMQECWRKEIAGVLHDLREKEKELRNKEEQLLRVQNEQREKANLLKIREQNLRERERVLIERELVMLQPVPSKRKHK *F KGKKNKPLQISLPTGFRHTITAVRDKAEQPGSPSFSGLRIVALTDGHKGKTWGPSTMHQRERSLLPSQLSGGQPEWPAQT *F STHSSFSKSAPNLDKKQQQQNQQQVASLTPPPGLGILGGSGGAGGTPATPLLYPAKIYHRARSQEYGLDHPLAYQPPPLY *F LVTDDSSETDTVASPTGCFHFLKSGNSSAASGAVHLHRFGGSLGNSPAVGRKKHSLDSSSHHPPANGSNSFALPNQLTLP *F SEDNNTYDHAFYRDVIKKMSMASSERVNSKSSGDLTMYNSSTPLTARDCDDAEEAFEGGRFQPDADADDECQVPASQMRQ *F NSTTSRKSSVTFQSVSFEEPDFVATPRTTARSDLYTSSASISFATYRSASPSLSSSSTTASASPSIASTEAVNGYHMQEN *F SILNTRRMQDVQPHPDVIKLRAQEQRQQTKNQKKQRPKHITKSKSVEAPVEGQHHEHDDHNDPQHQHHSAGSSKIRALFN *F LFTRSRKKYSKLAEHNMVGGPEFCAIDPYQTDLAMGGSSRSLKRKGKKPQTQSCEQLERC *F >Ilk.AA *F MEDIFHWCREGNSIQVRLWLDETEHDNNLGDDHGFSPLHWVAKEGHAKLVETLLQRGSRVNATNMGDDIPLHLAAAHGHR *F DVVQMLIKERSDVNAVNEHGNTPLHYACFWGYDMICEDLLNAGAQVGIANKDGHTPLEKAKPSLAKRLQDLVEKSGREVK *F VISFKEQSWQGLKTRSRDATLSRFKGISMGDLDLHTKLSVTPSGETWRGRWQKNDVVAKILAVRQCTPRISRDFNEEFPK *F LRIFSHPNILPIIGACNSPPNLVTISQFMPRSSLFSLLHGATGVVVDTSQAVSFALDVARGMAFLHSLERIIPTYHLNSH *F HVMIDDDLTARINMGDAKFSFQEKGRIYQPAWMSPETLQRKQADRNWEACDMWSFAILIWELTTREVPFAEWSPMECGMK *F IALEGLRVKIPPGTSTHMAKLISICMNEDPGKRPKFDMVVPILEKMRR *F >CG8789.AA *F MQPFSDSLSKSRDDLVTAASRQQQQLHGRRRHHGSSPNLSLDQTDNLRRSMACLQDEFGHLGIAATDLPFKSSDLDSPPR *F LQHHNNYAEITDSSAENTCQQRWPPHVGGAGAAFGHPDKPIGWMYGLLGCMKPVLSFIGKTGVIEVKSQRSEDWQIPFES *F ITELEWLGSGAQGAVFSGRLKNETVAVKKVKELKETDIKHLRKLDHENIIKFKGVCTQSPVFCIIMEFCPYGPLQNILKE *F EQVMLPSRLVSWSKQIALGMQYLHSHKIIHRDLKSPNILISTNEVVKISDFGTSREWNEISTKMSFAGTVAWMAPEVIRN *F EPCSEKVDIWSYGVVLWEMLTCEIPYKDVDSSAIIWGVGNNSLKLLVPSTCPEGFKLLVKLCWKSKPRNRPSFRQILSHL *F DIAGPELLRKTEKQYFETQKSWKEEVRSHLKEITQNGTNIHKYEQDLIKRRTAEWRHAQDIRMVYEDKLQKTNQLFFELS *F ECMSQLQEKEKEIAERERKLPGSGYKPNRRFGNTIRKMQHYRRRLNPAPAAIQQQSTTPDPETTPESPVKCMLYAQLDSN *F CQPKSYLANIIPSSGLGAPMPNKNKKVFRHRRNASGSFGAPPKYSPTRDRRYQSEPENRKVQLVERQTQTDAMDVSETDI *F SPSAEAPRSQPIDVPVPNHRQLPLQLQRVQKIAQAQARARSGSTSSAAGAVNPACPSNGNSLSTSELTYQDACSSPDQLI *F DDVMNSNERLDMTECCSDNENLERLGRKVIEFINENRLSIQSNTNSVSNADNGNGGASPLELRESGNSPCLSRCSSTHSK *F RRKHPLGDNPNGSSIGNANGESHEQDSWSDEEGETTDYKYALRRRSIGRQPIARGMRPRRSYKAPLSQKIAIHKRNVVIV *F SDEEENTSEYSHSPSSQHSTLESNTDIADMKKTQATSTSSNSYSEPEDDSSDSSDEEQGNRPTEAKAEGPALPMGAVRSS *F DIISIPTFEADGAVNMV *F >Takl2.AA *F MSAPVEGVPYEEIQTKELIGTGFYGSVYRAVWRNREIALKRIREGCEDKKIEREIYQLTKASHVNIVELYGTSRHEGCAL *F LLMEFVDGGSLSSFLHAKSKPSYSHAHAFNWAHQIAQGIAYLHGMQPKAVIHRDIKPLNTLLCEKGLKLKICDFGTVVDL *F SQSISCNAGTCRYKAPEVRELFDFKSNRIIINQPTGFQKVLQGNKPDEKCDVYSWAITFWEILSRKEPFEQYNTLFELYM *F AINEGKRARDRI *F >CG8767.AA *F MGDLLLNTPKRKQLLKDGPPVPTRCQVLGRGAYGTVFKAIYRDRSVAVKIIRAQAASTLHNESHLLNLEHRNIVRLLKLE *F SAADFGLVIMECPRGQSLQRIVDTLALPLMHRVLITLDVVAALRYCHSQNVLHLDVKPTNILVALGTKSSITCNSSKIKV *F KRSYICKLCDFGSSIEMGEFCAWQEPSVAKGTLRYMSPEALRSDTLTEASDIYSLGITMWQLQARRLPYHTLDCNETIAY *F QVVKHELRPDNYHQLKILALDSPIDCDWDLAHESTANVICRRANTSARRNLSLDPSYTVGRDLKKKRHRNRLALHFDSPA *F PEGSACSESAYSQLYKSCWVSAPELRLSSIQLKHELEFILCHST *F >Nrk.AA *F MVLKWGANLAVLGLCVFLFASATHANSLNAIEEPVTRRHHQRHHEREREENGYCAPYSGKVCKEYLTGQVWYSLEDPTGG *F WKNEQVTTALWDELISDLTGLCREAAEKMLCAYAFPNCHMEGGRAVKAPLCFEDCQATHLQFCYNDWVLIEEKKERNMFI *F KSRGHFRLPNCSSLPHYNASMRRPNCSYIGLTELKESEVSYDCRNGNGRFYMGTMNVSKSGIPCQRWDTQYPHKHFQPPL *F VFHQLLEGENYCRNAGGEEPHPWCYTVDESVRWQHCDIPMCPDYVDPNAVDLNTPIKMEKFFTPSMIFLLAGIGFVAIVT *F LHLMILLVYKLSKHKDYSQPAGAATAECSVSMRGGGDCGGNLNTSRETLGGNGNTNTLAKWGTIRSTATIHSNCVALTTV *F TNVSDAKGTKPNARLEKLEYPRGDIVYVRSLGQGAFGRVFQARAPGLVPDQEDLLVAVKMLKDDASDQMQMDFEREACLL *F AEFDHPNIVRLLGVCALGRPMCLLFEYMAPGDLSEFLRACSPYATHQAPTQDRLQLNELHLLQMAANIAAGMLYLSERKF *F VHRDLATRNCLINEHMAVKIADFGLSHKIYLQDYYKGDENDFIPIRWMPLESILYNKFSLESDVWAYGICLWEVFSFALQ *F PYFGLTHEEVIKYIKEGNVLGCPDNTPLSVYALMRRCWNRKPSERPGFAEINHCIQHSIAESECKAML *F >CG1227.AA *F MQSIGWTLIMKRGCLFSCRKETLNINGSRYTIRERLATGGFSLIDLGENASTRRSYAIKRITCHSIDDQNIALREIENCR *F KIDSENVIRVVDYELKGQADIVINTTSTLFIVLPYYKHGSLADHLQLRSRKQDHMPEAQILQIFLGVCEGLKAIHEAMPV *F PLAHRDLKTANICLSDSFEPIIVDLGSMTEARLQIVGQTDAQRLQDEAEERSSIVYRAPELFTVKTYCTIDERTDIWSLG *F CVLYAMCYFNSPYDPIYERGDSVALAVLSGNINIPEDSIYTEDMHELIKYMLRTDPMERPFVFSVIERTHDLIQKLEGRL *F *F >Nak.AA *F MKKILSKFERNENSRLENPPNSSSSSSSNEKNSFVGKVFTVGRVTVTVEDVLAEGGFAMVFLARGNGGGSSSATKYALKR *F MYVNNEHDLNVAKREIQIASNLSGHKNIIGYVDSSITPTGNGVCEVLLLMPYCKHHMLAMMNARLHVGFTEPEVLNIFCD *F IAEAVSRLHYCQTPIIHRDLKVENILQTDAGNFVLCDFGSATAKTLNPQQHGVTVVQEEIQKYTTLSYRAPEMIDLYGGK *F SITTKADIWALGCMLYKLCFFSLPFGESTLAIQNGQFSIPDSSKYSKGMHQLIKYMLEPDMEKRPNIWQVCEVAFRLAGK *F DNPVQNLHKTPIPNFDLLVVPPFESEAKRISAAASKAAKAQNVSVVEAGTSVAPRSRPKGSSSVHGGNPLGLGLPPSPSP *F RQNITSPQPQPQPVVEQFQANFPAMPPAVPPPPQAVTPAAPAASKLFESSGYPDPFNEPAAAVIPTEFLPAAEEPNKEEV *F PQDLNVIAGTPTKSMLTPPKPSGGGGHRRNVSDTSAFNKTFANETSQFLAPFNNNLAAMGDGQQTLASAASNPGIYASST *F ANSAAVSISELMKPGQTAVEKRVEAWNPFEEEQPFSQMTEDHIFEAEFDKIRQRGSQGTAPGAVPPPPIAAAVSPYPSQV *F SEDPFGSAPFSLPPGLREKASSLRKTGAKFIGVSSGGTGGGVATPIGLVAGGANTATSSNSSKWMLSPTLEVSSEEKTSL *F ISKLKTDSECGIPGAVGGAGEGVSGYVKLPVEDRNKYEKLRSKDQPTSDDSDSEASEFFQQDSDEGGGGIFRQMQMVTSN *F LPETIHKIQQVAYHKVDKTAHLPIVKKLRDKTKKPTTAAHQAAQQLNLMAAAVAAQAEEAAKAAESDGDSIGSASDLRAE *F DDDLFDENPRQTQAKQRRPLPTEMDGISESVKTCSSSAYHAECESVTTHEDDVRSRVVVKVRMRKKDRALVTATGDPSAP *F SEELSPTSSDLLHKFGDRPLLLDDELDYGSGESKSSTESPVAPILPTNPDVQESEEDVFALAPFKLPVGVPLKRKTKPKA *F KPKPSEPEMWTSTPVKSAAVGDPSTNFASFPAQLTPTNNVGNSAQLDEFNEPIFNPFQENAQNTQSGETNQCDLFGLEPF *F PQVIRTIEQNPQHHQIHQFPSHNNNNNVIKVPASVSVALPASVPVSVSVNSSAPQLVTINHSIIINKTPEPPMNYNNNNN *F NQTAKSSGTSSVYVNVPSFSSMSTSSCSINQPQSSPAAVIPVQAPLPVPVPAPVSVPAVPVPIPAVVTHLRPLLPIADNA *F YVQISQDRCSDFTPDDEEEPVVLRDADVVADGIGIPTPAKPKKEKSNLAVRVLPGKLTQKVKGHSYKKVSAAALGSTSSL *F SSGSKQKHQRLQYQSHDDDDDEQDQDENRGAGGGRNFQSNTIQNSAKTGFSNMSFEDFPSDQEMDRLSRTIPFEVVRNEK *F MLLEAEKKFGSLKRRNNLFS *F >auxillin.AA *F MGEFFKSLNLNYFSSSEVNGAAGNGAPEALGGRLDNDFVGQVVEVAGHRLRIKCVIAEGGYAFVYVAQDVQTGTEYALKR *F LIGADMQASTAIINEINIHKQLSGHENIVAFVGSSYTAPSTQLGAQYLLLTELCKGGSLVDCFRTNNAPFNPTCVLRIFY *F QMARAVASLHSQSPPIAHRDIKIENFLIGNDKQIKLCDFGSASTEVLSPTFEWSANQRSMLEDQLNTVTTPMYRSPEMLD *F TWSNNPIGPKVDIWALGCILYFLCYRKHPYEDGGKLRIINANYMLPPDPQYQCFSDIIRGCLKVNPFERLDITMVLEGLA *F ALAETHNWSLKGPLDLHIMPIESLPAESPLKSSAYSEVYTEPLSAAIPSSYNGHGSLLSSLRGGAGTLLKNIKDTSTKVM *F QTMQQSLARNDLDISHITSRILVMPCPSDGFESTYKTNNIEDVRLSLESRFVPQKLSIYNFGQRTEPRLPPPVRTVEAGS *F VYGCPQAHAPNLQGLFTVSADMYNFLNADPKSVVIVQTGDSGGCTAATVICALLMYADLLREPEDAVQVFAVKRHTINLR *F PSEFRYLYYFGDILRPTPLLPHYKNTTLVSLSCQPVPRMTKARDGCRIYMEVYCNGNLLLSTLQDYEKMRLYQAGPGKIV *F LPINLTACGDVTVVLFHARKGMVRPQGLKICQFQFNTGFIPEPETLITFTNQDLDDLPDPEQVTPRFCVSLSLAVTDSES *F PPSHKPPWMPAKPKRSPAALFSSDLEYAEMLDNFVTKPSTRSSPPPGARKSDRVSSPLVLPDVTETAHELPSPMPEPSPP *F IDLLNLNQQPSDVPAADPLTSAKPSTDASFDLLGAFGDDDSTGIGSAPIPDILPPPPLQQPQPKINNDPFDIFGSVDQGG *F VPSMKPSGFPPFVGSLPTFVAQPAATTNPSPTHPARASADPFANIADLATGLNLNFNRSTLSGKSPVNTSPQPTQFSSPT *F HKPSPSSQPQATFMHTPPTPQTLPSTSSIRTPTQPQAVPAQSRPDYSRLHFDSQKAAQQPGTGGSKNSDIFADILGQQGY *F SFGSKMNQCPRSINEMRKEDLFKDMDPKKVRIMEWTDGKKNNIRALLCSMHTVLWDNAKWQRCEMSTMVTPTEVKKAYRR *F ACLAVHPDKHNGTENEEIAKLIFMELNNAWTDFENDATQQNMFNA *F >trc.AA *F MMSSRTQDADGASIRFSDHTLDKATKAKVTLENYYSNLVTQYGERKQRLAKLEAQLKDESLSEAQRQEKRLQHAQKETEY *F LRLKRLRLGVEDFEALKVIGRGAFGEVRLVQKKDTGHVYAMKVLRKADMLEKEQVAHVRAERDVLVEADHQWVVKMYYSF *F QDPVNLYLIMEFLPGGDMMTLLMKKDTLSEEGTQFYISETALAIDSIHKLGFIHRDIKPDNLLLDARGHLKLSDFGLCTG *F LKKSHRTDFYRDLSQAKPSDFIGTCASPMDSKRRAESWKRNRRALAYSTVGTPDYIAPEVFLQTGYGPACDWWSLGVIMY *F EMLMGYPPFCSDNPQDTYRKVMNWRETLIFPPEIPISEEAKETIINFCCEADRRLGSQRGLEDLKSVPFFRGVDWEHIRE *F RPAAIPVEVRSIDDTSNFDEFPDVSLEIPSAPIPQGGEIAKDWVFINYTYKRFEVRNLE *F >wts.AA *F MHPAGEKRGGRPNDKYTAEALESIKQDLTRFEVQNNHRNNQNYTPLRYTATNGRNDALTPDYHHAKQPMEPPPSASPAPD *F VVIPPPPAIVGQPGAGSISVSGVGVGVVGVANGRVPKMMTALMPNKLIRKPSIERDTASSHYLRCSPALDSGAGSSRSDS *F PHSHHTHQPSSRTVGNPGGNGGFSPSPSGFSEVAPPAPPPRNPTACSAATPPPPVPPTSQAYVKRRSPALNNRPPAIAPP *F TQRGNSPVITQNGLKNPQQQLTQQLKSLNLYPGGGSGAVVEPPPPYLIQGGAGGAAPPPPPPSYTASMQSRQSPTQSQQS *F DYRKSPSSGIYSATSAGSPSPITVSLPPAPLAKPQPRVYQARSQQPIIMQSVKSTQVQKPVLQTAVAPQSPSSASASNSP *F VHVLAAPPSYPQKSAAVVQQQQQAAAAAHQQQHQHQQSKPPTPTTPPLVGLNSKPNCLEPPSYAKSMQAKAATVVQQQQQ *F QQQQQQQVQQQQVQQQQQQQQQQLQALRVLQAQAQRERDQRERDQRERERDQQKLANGNPGRQMLPPPPYQSNNNNNSEI *F KPPSCNNNNIQISNSNLATTPPIPPAKYNNNSSNTGANSSGGSNGSTGTTASSSTSCKKIKHASPIPERKKISKEKEEER *F KEFRIRQYSPQAFKFFMEQHIENVIKSYRQRTYRKNQLEKEMHKVGLPDQTQIEMRKMLNQKESNYIRLKRAKMDKSMFV *F KLKPIGVGAFGEVTLVSKIDTSNHLYAMKTLRKADVLKRNQVAHVKAERDILAEADNNWVVKLYYSFQDKDNLYFVMDYI *F PGGDLMSLLIKLGIFEEELARFYIAEVTCAVDSVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHNSKYYQEN *F GNHSRQDSMEPWEEYSENGPKPTVLERRRMRDHQRVLAHSLVGTPNYIAPEVLERSGYTQLCDYWSVGVILYEMLVGQPP *F FLANSPLETQQKVINWEKTLHIPPQAELSREATDLIRRLCASADKRLGKSVDEVKSHDFFKGIDFADMRKQKAPYIPEIK *F HPTDTSNFDPVDPEKLRSNDSTMSSGDDVDQNDRTFHGFFEFTFRRFFDDKQPPDMTDDQAPVYV *F >CG10951.AA *F MKKFRAKASSLPIFNGRITDATTLTTSSLQLPLGQNTQRKQSTCTRVLPTVFTITDGTTGAASTSLAEAMSSSKAQMPNR *F QESLLQLSVPRETGVGVAGPELANYEKVRVVGQGSFGIAILYRRKSDGHQIVFKQINLSELSPPGRDLAMNEVDVFSKLH *F HPNIVSYLGSFIKDNTLLIEMEYADGGTLAQIIAERQGKLHFPERYIIAVFEQISSAINYMHSENILHRDLKTANVFLNR *F RGIVKIGDFGISKIMNTKIHAQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILGEMCCLKKTFAASNLSELVTKIMAGN *F YTPVPSGYTSGLRSLMSNLLQVEAPRRPTASEVLVYWIPLIFRSLGKNKGYSYEDDVGGPGSDQLTAPVPAAAYSNVSME *F LELPTAQTETKQLMIADTAAPHEILEKRSVLYQLKAFGTCFSMAPIQLPPKAVIVDVAMSDSHFVVVNEDGSAYAWGEGT *F HGQLGLTALEAWKHYPSRMESVRNYHVVSACAGDGFTILVTQAGSLLSCGSNAHLALGQDEQRNYHSPKLIARLADVRVE *F QVAAGLQHVLALSREGAVYVWGTSTCGALGLGNYQQQQKFPQKILLSHVKTKPSKIYCGPDTSAVLFANGELHVCGSNDY *F NKLGFQRSAKITAFKKVQLPHKVTQACFSSTHSVFLVEGGYVYTMGRNAEGQRGIRHCNSVDHPTLVDSVKSRYIVKANC *F SDQCTIVASEDNIITVWGTRNGLPGIGSTNCGLGLQICTPNMELELGNNTAAFTNFLASVYKSELILEPVDILALFSSKE *F QCDRGYYVQVHDVYPLAHSVLVLVDTTTPLISSYEGDYPHL *F >Nek2.AA *F MSGEESAGMDFSQKTLQDYEVLAVMGNGSFGTCYKVRDKSTGELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQ *F YYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNGTILHRDIKPANIFLD *F AAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDVWAVGCLVYEMCALRPPFRGRAFDQLSEKIAQ *F GEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGIEVIIRHPLVVRNISELDGKFPILVDSGEDFYTLPSGARLFEDEEEDGV *F HPELSSTMFTEQYSFNEGYGQRRLSVTGVFTPDLRSELFYSAKRKIFPAKKLQLSDPSLYESIRREERAEERQVELAEER *F RRKKDQEQQKRDQELLKEAPSSPRALTQNIFDEVLKTRLHAIRAQESLLQQKLEELQTREQELQLAEQRVQTLERQMQEK *F LLQQEKHTCSCRQPIAPPIPPRKPAKSHHDDTYCTIELNETSPTVAKLNLATLPAPKSLNTLRKVTFKSPQKFVTYGIEN *F MPPPAVKPAVNPPPTGVPMQMSVSSNDSGDSQASSTRRKSILSLFGLSRGSKATSKSLPSVNQVAQAAATRVQRPPLAVK *F PPITQEQPAEVPPVANLWTKEQKKQAFELLAAMNAAEREASGGGVVGSGPGGARRQHLRQSVRERNSTLQRNRMRRSLVV *F ATGHQKLTTRDQMVI *F >CG4945.AA *F MSKKPRGNIHKIREFELEKIQLVDEFDIIQIVGEGWFGKILLVEHRGSQTEMVLKAVPKPYVTLRDFYREFHYGLHLGVH *F RHIVTTYDVAFETAGFYVFTQEYAPLGDLTSNVTDSGVGEVYSKRVAKQLASAIDYMHSKDIVHRDIKLDNVLIYRSDFQ *F RIKLCDFGESFPTGSTVERRNEWLPYSPPEVLEIKPEGSYKADPSHDVWQFGIVIFVCLTGCLPWQKAASDDPRYVRYLA *F WQGGLMMMPLRRTPRLFKLLTSNAQRMFKRFFARISNRPKSLADVTKFLDDRWLAKTAQKEMAEYETDELCPSMYSFHSS *F PDEKNKLLYTLADCGIETNVDRQQKKNRIKDWIESSIITEEDEEENEETNSASPSSSVSREPLPGHISSLRKPTSPAESA *F KEINSTLKDATQKHFDPRTGALQQGPSEMGQVAMAYSKSASPASNYSTTASTLNNADSLMTLGSRQDLLASNLTMYTSME *F TELNRLGEADPRLNQRTQSNNPLLTTFDVNTNPAAKNSIGVGTRGSSRSILKSSIATAAYPALGAFNHGQSHSLQQLNQH *F LQTSTGLTPSKSSFFRR *F >CG11221.AA *F MGTIEKRSFSFRLRRSFGDGGSTNSRNSNNNSSTCTNHNNQKRCSTPLTPTSTSTGRLEVPGAASVSRRSSIYKKPDKND *F GGQIHLIPDVELPLMTFADQYNIEKTLAEGCFAKILLCRHRPTNTLVVLKAVHAELTTIKEFQKEFHYNYELSHHHHILS *F AYAVAFQTMDYYVFAMEHAPYGDLASNIGPNGLHENACKLISEQLSSALGFMHSKNLVHRDLKIENILVFTPDFTRVKLC *F DFGATTKKGLLVHKVKHTWTSCVPPEQLELIKNERFQCLPVSDSWQFGILLYNILTGNPPWQSADWVKDQSYANFMKYEQ *F RKTTKVPDNFRRFSPRLMRCFRKYLSHDPEDRCKITEVAKYMKDRWVECRISTSKSATLISPTNHDQDSCIYLNQREGRL *F SGDENKLRFKRMMSTYGLDIPIDQAMVRRRVWDWLSTCDANFDPDVESLHALDLLQ *F >CG4523.AA *F MSVRLLTVRLIKHGRYILRSYCKRDIHANILDQNQLKTRSKRGFPLPSTAANVLRTTPQQAAKSVVNVVPRTINSPSGSP *F FNGSGSSPTSSSGIFRVGQHARKLFIDNILSRVTTTYSEDLRQRATRKLFFGDSAPFFALIGVSLASGSGVLSKEDELEG *F VCWEIREAASRLQNAWNHDEISDTLDSKFTIDDLEIGPPIAKGCAAVVYAADFKKDVASDGASLHTDAQPQATPAFAPNS *F WSTHEMMSPLQNMSRFVHNFGGSVDNVFHYSQPSAASDFVGAQSREQDQRHHEQQQHQNQEQEQHQNQEPSSSAFNVTSP *F ANSNINSSVDSYPLALKMMFNYDIQSNALSILRAMYKETVPARQRGMNEAADEWERLLQNQTVHLPRHPNIVCMFGFFCD *F EVRNFPDGHLLYPVAQPQRINPQGYGRNMSLYLLMKRYDHSLRGLLDSQDLSTRNRILLLAQMLEAVNHLSRHGVAHRDL *F KSDNVLIELQDDAAPVLVLSDFGCCLADKVHGLRLPYVSHDVDKGGNAALMAPEIFNTMPGPFAVLNYGKADLWACGALA *F YEIFGNRNPFYSSSGGMARERGEMTLSLRNSDYRQDQLPPMSDACPPLLQQLVYNILNPNPSKRVSPDIAANVVQLFLWA *F PSNWLKAGGMPNSPEILQWLLSLTTKIMCEGRPQMGAGLMPVASCGNRRAYVEYLLICSFLARARLRRIRGALNWIQNVV *F A *F >BcDNA:LD28657.AA *F MSNSQANAGISGSTVADEPIQHHPSLAAGPVSASCPAATPPSQSTQQPPPHIVSASTADAGSSAAVGVGVVAGSEGVNLD *F SSPRESGDDSEDESEILEESPCGRWLKRREEVDQRDVPGIDCVHLAMDTEEGVEVVWNEVQYASLQELKSQEEKMRQVFD *F NLLQLDHQNIVKFHRYWTDTQQAERPRVVFITEYMSSGSLKQFLKRTKRNAKRLPLESWRRWCTQILSALSYLHSCSPPI *F IHGNLTCDSIFIQHNGLVKIGSVVPDAVHYSVRRGRERERERERGAHYFQAPEYGAADQLTAALDIYAFGMCALEMAALE *F IQPSNSESTAINEETIQRTIFSLENDLQRDLIRKCLNPQPQDRPSANDLLFHPLLFEVHSLKLLTAHCLVFSPANRTMFS *F ETAFDGLMQRYYQPDVVMAQLRLAGGQERQYRLADVSGADKLEKFVEDVKYGVYPLITYSGKKPPNFRSRAASPERADSV *F KSATPEPVDTESRRIVNMMCSVKIKEDSNDITMTILLRMDDKMNRQLTCQVNENDTAADLTSELVRLGFVHLDDQDKIQV *F LLEETLKAGVMSDGAGAESSGAGVTTTATMAALEQLERNWSISSDADKQGTAVMYVPQEQQNADGDVDVEHSGTTSN *F >png.AA *F MELGDSKLRAVRVLGQGTFGRVFLCHQNEVRGQPERKVCVKRIIVRNPKTELGLIKEEVYIISQLRHPHIVEFLRSFSHA *F GTVNIVMEYVPNGTLRDVIQQLPSGTGGVNQERLMGYFRDMVVGLEYLHIRCVIHRDIKPENMLLDANDRVKIADFGIAN *F VHAPSTQLQAGMGTPMYMAPEAMSSQGKVDFKSDVWSLGLVLYELCLGRSPFAAFLDKNATPALLHTVVQALVRPRLDCQ *F LIRRLYDPVWAQVCELMVVYEQERRICLPDIFHLDAGMTVTLYKHYFSYSY *F >Pvr.AA *F MAMLPRLILLPLLLILRISWSDAVPLQQFSPDPDDSIENCGGENGAPLMTPCKSAIILDAQTSTTLKCEDDEPMSWWTSQ *F SQYVHVKSFDNTEDPARPFGTSLHLIEVTADYVAAYYCVKTSKFSQIAKEEQSDEAMIELVNQGYASSIYVYVNDPDTKL *F VDSHNVVTARQYTDVVIPCKPAMPDTEVLLETSNGEMHSSKSVGRYDPQRGFTIEIRSIVDGGDYYCRPNPPFPHNEEEM *F TSIEVRFIGNGHIDKDGKPLPKPVIRSSVEHHVFTDTNFTLDCEQSAYVESVYGMEWFTPSRDENRIFASQSRTDPKTRN *F STHQTGRSTLTVLNAQPSDTGLYKCVTTDNSNQNVQRATYRIKVLKQNESYLNVGEPSGHYNVQEYANRTIQMTANFEGF *F PTPSFSWFKPDGTEVRQSENNFKILSTELSTMLQVLNAQLQDSGTYVLRGSNSFGVVQREYNVSVMDAPALKMSDAYVQV *F GSVARLECTVRSYPPAIVTFFFRPCSLEPQWPTCSVLNQNFSLPSEQEKYQFQTRPRPGKLSVERIYEVSFLPTEPGILT *F CIAQNIIDGKERRTLTKAHVLLGNISENMTIYGFDKDHKIAKEDNVNFTCEALAYHFDGNLKWFINGEDLKESDSVHIET *F SHTKYSYKSTVHITTISDRDRGTYECRAYHNDKDAAYSSREIDLYVHDPSAPQWTNGGQEGHSKIKRKLSQTLELECAST *F AVPVAIVRWFKDDKEVTESKLRHIIEKESKLLITHLYPGDEGVYKCVVENRLDRIERSFTVVISDLPGISMAWVWFGVIL *F FLILIGLCVFLAVRYQKEHKRHLALKAAGLANFEEGAVGHINPDLTLDEQAELLPYNREFEFPRENLKLGKQLGAGAFGV *F VLKGEAKGIRREEPTTTVAVKMVKATADNEVVRALVSELKIMVHLGQHLNVVNLLGAVTKNIAKRELMVIVEYCRFGNIQ *F NFLLRNRKCFINQINPDTDHIDPSIMTQRMSDNYELHRDTNGGGLKYANVGFPIHSYINEPHNNNTQPPTHRRNSDNDPR *F SGTRAGRTGSGTATYSYDRQMDTCATVMTTVPEDDQIMSNNSVQPAWRSNYKTDSTEAMTVTTVDLISWAFQVARGMDYL *F SSKKVLHGDLAARNILLCEDNVVKICDFGLARSMYRGDNYKKSENGKLPIKWLALESLSDHVFSTYSDVWSYGIVLWEMF *F SLAKVPYPGIDPNQELFNKLNDGYRMEKPKFANQELYEIMLECWRKNPESRPLFAELEKRFANMLGEDVASHYLDLNNPY *F MQSNIEYMKKQSTDYLALMGSPDELAPAAPRYVNGHIVPDIRIEELPDDYMEMSRDSDPDACTAIFSPTRLEGESSDFPD *F FSSETTFNFPGARQSPTLSNNLNSGSSKPLRKKNGMPTVDVADQAPEEIPMLHRSSTGSDGSPEQGRRFNQALKQQYVTP *F TPSPRHHVETKLNGEPSENYVNMKPPRKNIPGKTTTGGGGAAAGASTEAFSNPSYQPLSTVNEKEQRRY *F >CG3277.AA *F MHHSTKVPSRIVLILLLVLVRNAVGQAPRENATNPTLISLVRSSEKEPEQMESHRYVTGLSEPIAMEPLTRMRLNPNPDG *F EITELTTNVTLYMEKIPRLQVRWQDNLPQGTSYNVLVSAVNNSRCPDAPCSEYGIKEKNASAETHSVNLPVNPNLNVESV *F DCNYMFGCQYQVIVETSNAMVRRSARVYIPQCLEGKCSCQLAPTPPKVLAVAKMLSNETISINLSILASELLRKEQDSHL *F HETLRTYKMQLNELNFRATPKGFEGVMKLGLGTQLKEKASLSLQAVIIDPTGCEGPRSVTKVPEQLHNCDERHADCHYPS *F WPDYAASSSPKSQDNGQAAQAGADLHPDLRHLRHRKSQVLGLADIFEVPHSAIQIGRMLGEGAFGQVHEATAINLRRMRG *F TTIVAVKQLKPNPKADEVAEYLAEIEMLKGVGTHHNVVSFLGCCTIKPPYLMIMEYVNKGNLLSYLRTVRKEASKLRNRN *F ANYTSCRPIMTRTNSTSSPKSQSVNYIELKASSQTIEMPQEDSTAHMNGIRQPHPSFAESKLVALGLTSWAKSVNENLIS *F ATYTIVEDEDAFEYILDNKELHNFALQIANGMRFLEEQEITHRDLAARNVLIDSNKTLKISDFGLSRHGIYTNTRTRKLP *F LRWLSIEAIRENVYSSKSDIWAYGVVLWEIGTLGASPYPTISNDELIPFLMAGNRLERPEICTPQVYTIMLQCWLEEPEE *F RPTFDALYKVLSPKTTYVDINSLSDDYVFPPISENRE *F >PDK.AA *F MRLFPVRFSAASLSMASLAKMLDFYSGFNPSPLSIKQFMDFGQNACEKKSYIFLRKELPVRLANIMKEIALLPDNLLHTR *F SVSEVSSWYVKSFEDVLVYEKAEPTHDNLQKFVADLDLIRNRHNDVVQTMAQGVIEMKENEGGQVDAPTESSIQYFLDRL *F YMSRISIRMLINQHTLLFGGNPHAGGRHIGCLDPACDLSDVVRDAYENARFLCDQYYLTSPALEIQQHSSEPGDNLPIRT *F VYVPSHLYYMLFELFKNSMRAVVEHHGHDNNDTLPPLKVAICKGKEDICVKISDQGGGIPRSQTDQLFKYMYSTAPQPSK *F SDLHTVPLAGYGYGLPISRLYARYFHGDIVLLSCEGFGTDAIIYLKALSDEANELLPIFNKTSSKFYRATVPTGDWSNQV *F KYAKKKKTSAVSQ *F >PEK.AA *F MQDDLDGIVRHRRRSLSFLQIVTLTMAGLVAFDPAQVLAGHPTTDSELQTAGSPRPPGLEHCVDQEERRVARRLLYISTL *F DGRLSALDIAKSGKLRWSVPTGPGPLISSSIHRLELTNNGQFVRMIPSLSGGIYKFDGDSIDPIPITAEHLLSSSAKFSD *F DLVISGGKETRSYGVSVRTGQLLYECSLNGCVNSTEEGLAIDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIV *F RRQTQTVRAVESRTGVERWNFSVGQHELDLVRPSECQLQPRDELELAVLDVDIKVVVPEGIICAFSKSEPQTMLWKYKFD *F HPIVSAWNTNADDELQPIDLFSSAQWLWDQDENDTELPNAPQSPPSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTG *F DTSLMPRIPWKPISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDENFAVAAQSVLNASEFVNGNGFYFYTTGDLN *F GPQECSTQNNPTDLPAITAPTSPTNATSEGTEATGNHSVNDDLGFSLDDIDAPVKVVILSLWFWWKEIVVIAFTSAVILN *F IFMGQRNQRVEREYLVIERHVPVQTAIEATEASTQALLGPVVPMQRPGNRFSFPPGQANQRTISESTTDSGEHYTSRFQS *F DFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIVRYFHSWTETPPTGWQEEED *F RKLLAHELSTSIQIETPDDSTMPSLTEQLKEKRQQQLLSWVSDAANSTACSHDFHLPGESSLKNIREEYDYDEEEDSLIE *F FRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFDLKNINYSQHQLVSNSFQIESVRPKSSGSDD *F ANDDNKARRKPLTLALAQNHNNNQNGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKESLRDWLRDNRSETRAAHIGDI *F FHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPE *F QLLGQHYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQ *F LRNILQLPHLLSEGQSEQAELAERARRLSRSRTFSSSSEPHQ *F >Gcn2.AA *F MADEKAKESFRERQAQELEVIKSIFGCDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLP *F PKISLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDIQRQR *F ESLQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQ *F IQRGCCVGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVMASIEKQVSSLSQ *F LQHKNLVSYECVLCIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVARGVLDALVFLHNKGVSHSHLLDTTVFM *F DNTGNVRVSDFSLVPNLLELLSGAGQSSSCGDLPALGALVESLMPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVD *F NGQQQVMPLPQQQHPNTVQRTGSAMPYQIPTLALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPARS *F RQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQSQQDLSVKPAKSPQLGPTLEEDEDEEDSS *F SSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEDSTRGKTNSPKPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDR *F LWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGT *F TLYVAPELTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLN *F HDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAII *F IDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSTANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNS *F VNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAIL *F IFCNVPKAQYGALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEA *F ASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDRASNGGIVWQMTADLKPNRSGHPSVLAIGERYDSMLHEFQKQ *F AQKFNPAMPARGVLSGAGLTFSLDKLVAAVGVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASEL *F GDEAQDLARLGALHVILVAENGSLRVRSFERERFQERHLTRTELVEFIQKMLRSDGLNGTTVDNFSQLSALGSGDNRSSG *F GKERERGENGLSTSASNATIKNNYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAV *F VNAIVGAINPREIRKRETEPEINYVIERFSKYKRYISEINEEVVDYLSDAKTPIVALYSISDSYYRVII *F >PhKgamma.AA *F MAKDEEDDLLPDKDAAKGFYAKYEPKEILGRGISSTVRRCIEKETGKEFAAKIIDLGATTESGETNPYHMLEATRQEISI *F LRQVMGHPYIIDLQDVFESDAFVFLVFELCPKGELFDYLTSVVTLSEKKTRTIMRQIFEGVEYIHAKSIVHRDLKPENIL *F LDENHNVKITDFGFAKQLQEGEKLTNLCGTPGYLAPETLKCNMFEGSPGYSQEVDIWACGVIMFTLLVGCPPFWHRKQMV *F MLRNIMEGKYSFTSPEWADISEDPKDLIRKCLVVDPSQRITVKEVLRHPFFNQMVLMGDRRHPAPPIAPAQTNSRHLLQP *F EASSYRFGQLNSSCAGAPNYLYCAPQSSYSSNRMRDGALDDAGASAASATCHVMSPSNASNSYATNTTANTSNSALASAS *F SHKTLTAMVYYQQQPQQHQTQQQQQQYQQQQTTQLPVQLQLQHPLLQTGGDGKQSVYAPPTVQLRKQSRFNARKKFQFAI *F LVIRAIIRIRRLRFTAEPLHVEEAIRDPYRVKVLRKVIDGCAFRVYGHWVKKGEGQNRAALFENTPRTELHALYINNLSR *F *F >CG4549.AA *F MKNAIHPENCNGAGTEEEASSAFHAEIDRVLLNNNGNHGDSSNEGGGGNGSGRGGATGSGNIAGLGGSESMWSPGGGKSH *F DVAQAFANALLLRNMNHVVGKGQPVVQNHRKAYQCKGDTINPMANGEDLRLSKIIRRLINENNPTVSLELCSKLDQAVRT *F PINMGYMTCSFVWILDNMLTLYKQCPPPVLEECSKTLGLIGFINRKSYPIYEEFIVKNYKSSKRMQKYMIMALRATLSCD *F TKCELHMYADKIMLLLKDFLENAESADIFIVVSNTLVQFAASYAETFECHFTDVVDIVIGWQLEAGQPTDLKTHCAQVLE *F QLTPFFSKQIDFSYGLLDQFVEDITTLEEGEPANTAERVGAFVGAFNTLLKCLARMQIFVGMPTCECIVKMAVDHLIKIM *F PTLHLNTEALVNINELICICLLNNFTGLDPILLEQVLLDQVKRMISLTELQRQSVLYLLLCTVRRLRARLTPSLVHFIFQ *F SNPYMTKVRLRSPGETSYKLLLRTCQETLLIRNVPLLQQAYKYLVDDIDACLEKLLITAPRSKARKASVLLVFHLSALAA *F LAKQTSSIIGMYACKPSILELLLTNCRAHELKFWSKYPAAQQAIFGLLVVHCQANHNFRTNSSLLRDQELSAENTSPTAN *F SFASILRFLDSVLGQAHQLAPQNLRVLLQWIQMLLRECREKIDLLMEQENFRGICRNIAATASKLVPLESAACIQTVLDY *F GLERLEKYPKLLILYRDTALQQLQMLSTNYHAPYFQIYAQLPLHLTLTGGESSMPGMASRRVSVWQQRISQYSAVRDNVF *F RDFFDRVQKPEQDSLIHCLRELFVRSCQVAPQDERQMNLSQCTKRCQRLAIAWLQFEAARYCVDQRLRTTVGKPQETFLG *F FEAIIMRHARLLSGCAKEIERSALDDLSLEELLSMQSNLSLLLGFLDALEKLIYNAAEGSAFALRPPEKQVAAFFRLNNP *F TCQSWFNRIRIGVVIIAMHVQQPELVIRYAQQILVNSKTQDPTYSQAIVYMAWSLVSCQEADSLRGLRLWARGKSCKSYK *F WLKYAADQAAGKRESALAGYRTILAEKELQSELEPHTRQFVVSQMMQCLQDLGQWSQLVELKQQQMTRPEDRELNPFLQR *F SNVEVNALERLLAKSEESCSSMDALGGVFQQLSLWPSNWDESVSSSGLSERASFSSIHMRQRTEDIVLHKLLEDRCVPDQ *F AKNLLDTQWRDSLLNPSFDQRSCKELTLLRHIVQGVSGGQELSLLPVSSGRCQNRSKFISSAILMRCLAWTQLLRQHCAP *F GSWETLCLDAAAAAREEGNLQLAETLLTQFFGQPIGEIAALFSLEQGVQTDNPEMLRGYSELVKCLHLQQQQSQTHSGDL *F SSSIDVCAALCLNIQKSNNQPAAGADLLLNLADWIAVRTCNGLTTNQSPVLIQLLDQLPECPLTCDSSQPLAIPQAERMV *F ARLVHSCLQQRPNYAEALIAYGNWCYRWGKKVADSCCVLTQADATAISQALDIPQPLESEKLDELLQALSTEQPPANCVE *F VCPDAARARDDEAAKNRLRRLTFLADKTPEALDAILQIWRRAIANTYDYYKDAARSYFQYLSFKSGSGPEKPEGEGVVSQ *F RERLHVDDSNLVTTTLRLLRLIVKHASGLQEVLEQGLHTTPIAPWKVIIPQLFSRLNHHEPYVRKSVCDLLCRLAKSRPQ *F LVIFPAVVGANREQQDATAPPATARPTTEDACCYGYLLGELSKQAPEAVQHVKLMVKELRRVCLLWDEYWIHSLAHIYNT *F YVSRVSALATDFRPDDHEGKNNRFNVWRPQLLADLEALVAVTSRPPETTYERSFRKRFDAPIRLTVDALRHRRYPEAWDK *F LKQLYHILQSNMIRGSGSTLKMQSISPVLCGIGRMRISMPGLDAHGPDGDQVYIESVESSVCVLPTKTKPKKVAFYGSNG *F QRYTFLFKGMEDLHLDERIMQFLSISNAIMACRSDAPGNGCYRAHHYSVIPLGPQSGLISWVDGVTPVFALYKKWQQRRS *F QVAGNAGAGAVANVPRRFTDLFYNKLSPLLAKHNMQVSDPRRQWPISVLLQVLDELSQETPNDLLARELWCQAGNAAEWR *F QSVRRFVRCMSVMSMIGYVIGLGDRHLDNVLINLGSGDIVHIDYNVCFEKGRTLRIPEKVPFRLTQNLVQAMGITGIEGP *F FRLGCEYVLKVMRKERETLLTLLEAFVYDPLVDWTTNDDAQALRRSLNAKLQESADGGGAGGLGVGDLKYHKKDKNKGKP *F LDSDVKRQPFLSKLGMLQKYWSTNKTELMPQLEEMEQEVGNLQAAQAKQVVAEEELVKLNQRSALIAEIKSLGTAIESHS *F FNTASLRNAVRRGHSEALALLSTERLPDFGRVQCILRSYGQCLQLYHLLDLQGQLVKLQMESNSENAREFSALTEALQLS *F GLDSMRSQLNELLGRMDMVAQKSSKHLQEYAGVMNFYPEQSHRQNLFVRFHDSFATYIQNGYTADSTTNTNSPSSSIICK *F ADVVGVAEAMEYSWERLGCQLHEASKLYAANQAQALTLGAPTTALLSMIVQSGCSQLLLKASLVRTLDRAGGAFAAYEQV *F ALASHDDGLLHHQLLFIHLVRTMLQGVLVMTKEEDQHLAQLESLLSALSHLKKMFEYDLPANLYRLLLLQPNLGKLSALC *F HLSASSLAQLFLEATMENGHKPPDQFPVERRFLLTLQPVYDQFLLASTSLDSLVSSMQSMLEDVHDVQTQQIMELGLMRS *F CHTELNDECFFGLVSEALESSRTCDVREMARPMLGFIHRLQVEKLAGLLPILTRNFYTAVGPQCLPTASCGDPAQADHLC *F ESLFISLQSDGALLQQQAEIALLSQQVDLHTLAASAQYWAYSEALGSQLRCGPHIVSRPKLTAAIGECWLELDQKLTALQ *F QLQAGLESQLSQLQTQRSNWNRNHIDNLLRMEQCNKQRTMSHVALLQKMTDGAGAVARLEQNAIVVGEEGQALVDHLEQW *F LAAHGQWQASSSRISAVEQSMVELLDPEGAIDHYWLENVQGLLEEQTCKVHREIAAIEGEQQSKHRFICTLLKETLRLLE *F NMPRFHVQSLCSEAQAQGQGKMEYANVQLLSDHLREGQGLMQSLYMRLQELRKDICSDRRVLQPSMLQNWRHQLEMILTL *F AKQEVNEFFKGLEDFMQHAGETDSYEIFTHAKGSGNVHEQKRNAYGVSVWKKIRMKLEGRDPDSNQRSTVAEQVDYVIRE *F ACNPENLAVLYEGWTPWV *F >Tor.AA *F MSTTSVVQQFVNGLKSRNRNVQNKATQDLLFYVKTELREMSQEELAQFFDEFDHHIFTMVNATDINEKKGGALAMKCLIN *F CEGSLTARKGISPYLNRLRDLLLINDVSVMEIAARSLVKLANMPTSKGADSFDFDIKKAFEVLRGERQEYRRHSAVFILR *F ELAIALPTYFYQHILTFFEVIFNAIFDPKPAIRESAGEALRAALIVTAQRESTKQSSEPQWYRICYDEANGSFNADLGSS *F KDQKGVTRDDRIHGGLVVFNELFRCANATWERRYTSLKTLFPKTQHNKFLEASSSSSMGSQLNTLVPRLKVPFIDKLGST *F QTHLGEGEHHKGVAKFASHNVLESAYAQEILQEHYTSICDNVLEQRTSKSPYVQQALLQILPRLAAFNRAVFVEKYLQTC *F VSHLMQILRGKEKDRTVAYITIGYMAVAVQSAIEVHLSSIMTSVKVALPSKDLTSKRKVPVDPAVFACITLLAHAVKSEI *F ADDVKDILEQMFYTGLSPALTVCLRELSENVPQLKSAITEGLIGILSQVLMNKAAILPYTALPTIAIDGSLMQNGDGATT *F VLALKTLGTFNFEEQNMLDFVQRCADYFIVHEQQEIRLEAVQTCTRLLKLAVQSSESMENSKTLSDTVSHVIERLLMVAI *F TDMDCNVRIRILRSLDETFDGKLAQPESLNSLFITLHDEIFEIRELAMVTIGRLSSINPAYVMPKLRTTMIELITDLKYS *F GMSRNKEQSAKMLDHLVISTPRLISSYMNPILKALVPKLHEPESNPGVILNVLRTIGDLAEVNGGSDEMELWADDLLSIL *F LEMLGDAGSPDKRGVALWTLGQLISATGRVVTPYHKYPVLIDILINFLKTEQRRSIRRETIRVLGLLGAMDPYKHKMNKG *F LIDSQKDNVLIAYSDGKVDESQDISTAELLVNMGNALDEYYPAVAIAALMRILRDPTLSTRHTSVVQAVTFIFQSLGIKC *F VPYLAQVLPNLLDNVRTADNNLREFLFQQLAILVAFVKLHIISYMGDIFKLIKEFWTINTPLQNTLINLIEQIAVALGCE *F FRDYLAELIPQILRVLQHDNSKDRMVTRRLLQALQKFGSTLGYYLPLILPPIVKLFDSPYVPQQVSMVALETINNLACQL *F DFTDFSSRIIHPLVRVLDAEPELRDQAMTTLRSLAKQLGKKYLVFVPMVQRTLNKHRIVDPEYEELLSKIKSCSTLADSY *F GAGESELRPSRFKNNEPFVTDRNSNNKNLQVTTNELRTAWQVTRRVSKDDWVEWLKRLSIGLLKESPSHALRACRSLAQE *F YDTLLRDLFNAAFISCWTELSPDLKNELTQSLIQALQVTDMPEITQTILNLAEFMEHCDRDPIPIETKLLGTRAMACRAY *F AKALRYKEEEFLLREDSQVFESLILINNKLQQREAAEGLLTRYRNAANELNVQGRWYEKLHNWDEALEHYERNLKTDSSD *F LEARLGHMRCLEALGDWSELSNVTKHEWENFGTEAKSRAGPLAAVAAWGLQDWEAMREYVRCIPEDTQDGSYYRAVLAVH *F HDDFETAQRLIDETRDLLDTELTSMAGESYERAYGAMVCVQMLAELEEVIQYKLIPERREPLKTMWWKRLQGGQRLVEDW *F RRIIQVHSLVVKPHEDIHTWLKYASLCRKSGSLHLSHKTLVMLLGTDPKLNPNQPLPCNQPQVTYAYTKYMAANNQLQEA *F YEQLTHFVSTYSQELSCLPPEALKQQDQRLMARCYLRMATWQNKLQDSIRPDAIQGALECFEKATSYDPNWYKAWHLWAY *F MNFKVVQAQKSALDKQQPPGASMGMTMGSGLDSDLMIIQRYAVPAVQGFFRSISLIKGNSLQDTLRLLTLWFDYGNHAEV *F YEALLSGMKLIEINTWLQVIPQLIARIDTHRQLVGQLIHQLLMDIGKNHPQALVYPLTVASKSASLARRNAAFKILDSMR *F KHSPTLVEQAVMCSEELIRVAILWHEQWHEGLEEASRLYFGDRNVKGMFEILEPLHAMLERGPQTLKETSFSQAYGRELT *F EAYEWSQRYKTSAVVMDLDRAWDIYYHVFQKISRQLPQLTSLELPYVSPKLMTCKDLELAVPGSYNPGQELIRISIIKTN *F LQVITSKQRPRKLCIRGSNGKDYMYLLKGHEDLRQDERVMQLFSLVNTLLLDDPDTFRRNLAIQRYAVIPLSTNSGLIGW *F VPHCDTLHTLIRDYRDKKKVPLNQEHRTMLNFAPDYDHLTLMQKVEVFEHALGQTQGDDLAKLLWLKSPSSELWFERRNN *F YTRSLAVMSMVGYILGLGDRHPSNLMLDRMSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLIKAMEVTGIEGTYRRT *F CESVMLVLRRNKDSLMAVLEAFVYDPLLNWRLLDVDKKGNDAVAGAGAPGGRGGSGMQDSLSNSVEDSLPMAKSKPYDPT *F LQQGGLHNNVADETNSKASQVIKRVKCKLTGTDFQTEKSVNEQSQVELLIQQATNNENLCQCYIGWCPFW *F >CG6535.AA *F MSALLNEIQRILGDLQSGKAASRNKGIQQLDEKLSSCREDLDKLFLSKTSDISWTTIFEASKEALFKQAGNLEDVNEKVF *F KTLAGKNYLYDNVLEKITQFNLEAGSQTSGNGHFLAKTSIFSAFEDGIKMRVVVKYFGDRILSLLEKGIYSSVSYVRDLK *F INEYSRILSYLFELNVDMDEVLRTRILKCITRTVSLAKDRVQLHVDLVEYLPELSSFALSAFGARKTEIVRVYLIFASEL *F AVNYNHQLNVHMQEILPKLCEYHDEDAFRDDTRNLFFQCVSKSLHSMYLKMDMCDFNTLGVPVHEKWPQTLLRLKTIVNV *F EIRKNSWARCKNALLSNNKFSDPFIKMSALAMYIVLWHLETKKADENGEGDAPKKIPKPADKMETIFSLIDKKENTFNDV *F WLAIFTEILQLSSVILNVANYQMALTTVAEIMQMYGNAKNLRNLRLCLAHLLTKEQELLHSKSIREDFLGELWSQMANQL *F ISETTTNSEEIKEKQLVLQMLIRHNKLNQKLSSTLLNNIISNEMLKRNECLATIREIFIHADKCGQDKASADLEPIIAWA *F YGSADRFIAAQMIHNIDSIDAQLQADTFAISIINFLDVQQLRQISQSEHIVPSTERNLLAYKYNEQLICFDKDYATPFEI *F AIKKSYTSNLTAKIVRCVGLIAQRCPDIYLLENFAVICKSTAKFITMPTLEVRFATLFTFTILLESNCVTSDAIGHSRTH *F WDFCQELYESIEFKKLTYNNEDAIQNSNALIVQMLIAIFLRSSFHQEIALKELLHHCALRRLTEKDIERCSKSISEELAL *F PILASFLLIKNSSCSESEGQNFKEHLQLLSENLSYSQLNATDVDLDLDILCYVISMLHDPQEMMRLFGSLAICNRTASWY *F SLSGESLFKCLNFHIDPEMRPSMDSRIQSMTTLQTKHSRVLVDIFGRLKTNCYSASFSSQALHDFFLYCEVADAVYDAAR *F KNETIVTQCSFFVRDIWFFVVRLLIHTKFIRVQMAALTFLELLLNKHNFRLDDYQNHFGDIAKLLSNFQLCCEAKEVREK *F TMSIVMYILESKKGHINLNSFLEETTDCEFLKPLREDCKSSQPNIDRADVANYLRSFLLSPTPERLRDLRTYIAEHKDKV *F QEHEKLLFGVINKLIQMTRDAHNKTTSIDSLKCLAQIGPLKISTVSYYFQTDFEAFEKSNGEPMEAFLGVVCHSLDTSLF *F QFDPKTHEGLVSVAIQVVNSKPGSNIMERYKNLRIFADKSTASTFLHSNKQIRRIDWLSILKATKSLSYEPWMCAFVSKV *F FQMCGWLGFDKLAATSFAFAKTCLLPFIKLLLENSLEHVESLSQMLDYFFEGFTSSTAPNSQEIFRNKRAIKKFLHICEY *F IRIFNNWTIPINLSNVVMASNHCQAYFLSIMYLELWACSESPKSKADFLDNECFQDGAKKAYESIGCLDAIPGFVNPMRS *F RLDFLGHGSNLSTILLESDHLDRASGQLCIDIMKGNGLWSFAKLQQHQNIEPDYEIFWRLGQWDSLTDPKHQQNQTVVRT *F SLDLEQEFKRHHFVALRSIGQREEENSLSAIEQAYSCVRDILMEISVECLQSVYKYLTWLCSLQQAEDFCQIQFGTQLDP *F ASTTKIFRKWQTELELKYGNFSCKEYVIAHQIALLKLAGTRASRRMSEFYQKDPISTYLMKGIEECKSAGKLNLAAKYTA *F TLRELPNIRESIKISVLLEDAEINLKMGNQQIAKAILDYVTNNNEFVYCVQRVPALRMQGEFLLDCNAETLSWVQSHKFN *F NSLKLIDDFVQHRQTLSEKYRDIFDWHQLDAYASKQRTAAYATIAKYADREYQQLHDYRHSQEYQTLKDIIEQNRQTAEK *F VTQRENQDRRVISVQMKRYASLDEQQLNQIEEKLTEYLRLALTNYMAYCRLDSGFSSAAIYRIISLWFTNATSKQCQECI *F KDEILTVPSYKFICAANQLTARLNSKNTSLLKGLTDLLVQCGKDHPYHTFYQLYPLVFAHLDGENSNTERSGIARKIIAM *F ICEKNGTAGECSKQLESLLPALITFANEGKTNDNRPVSDSVRNKQFDKVRRWRNLNAVHCPTLELPVMPSKEYSIISVVK *F WTNETTQCGGLNAPVKIMCVCSDGKIRAQLVKGKDDLRQDAVMQQVFGIVNELLNQDSEFIERKLKLRTYKVTPLSMRSG *F ILEWCTNSVPVGHYLVVEGKGGAHARYRPNDWNNNKCRKLSSDHLKSPKETRYAIYKKICENIKPVFHYFLLEKFPIPGV *F WFERRLAYTNSVATTSMVGYVLGLGDRHTQNILVDQQTAEVIHIDFGIAFEQGKIQTTPETVPFRLTRDFVAPMGICGTK *F GVFAKSCEATMHILRRYKSVFTTILEVLLYDPLFIWGVLKKKQSPQQSGEESVNLVAQRALLLVQNKLDGREAGTMGDSN *F VEAQVERLINEATLPSNLCMLFPGWDPHL *F >CG2905.AA *F MSVIENVPVNTFRNYLNILNDSSSKDELKLKATQELSEHFEMIMQSPAYPSFLDNSLKIFMRILQDGEPQFIQENTMQHI *F RKLILEMIHRLPITESLRQHVKTIITMMLKILKTDNEENVLVCLRIIIELHKHFRPSFNSEIQLFLGFVKEIYTNLPNHL *F TSIFETSNDVWVTDLKDLNLEVLLSESYSVRTIHVEKALDSNSQQQIYNLLPRGILSLKVLQELPIIVVLMYQIYKNAVH *F QEVSEFIPLILTTINLQPTVTRRNSPQKEIYVEFMGAQIKTLSFLAYIVRIFQEVVIASSLSVTSGMLNLMKNCPKEAAH *F LRKELLIAARHIFATDLRQKFIPSIEQLFDEDLLIGKGVTLDSIRPLAYSTLADLAHHVRQSLNIDVLIKAVNLFSKNVH *F DESLAVGIQTMSCKLLLNLVDCLRHHSETEPQRSKALLSKLLKVFVKKFETIAKIQLPLIIQKCKGHAFSGALVNSSGNA *F SLSHINAPDLKDDISNIQVSASGSQWIYSVNVAEFRSLVKTLVGGVKTITWGFFNSKFQLTDTKLANHEKIFGPEIVCSY *F IDLVYYAMEALDIYTINVNPNQQRTSGLISRSKEEKEVLEHFSGIFLMMHSQNFQEIFSTTINFLVERIYKNQSLQVIAN *F SFLANPTTSPLFATVLVEYLLNKMEEMGSNLERSNLYLRLFKLVFGSVSLFPVENEQMLRPHLHKIVNRSMELALISEEP *F YNYFLLLRALFRSIGGGSHDLLYQEFLPLLPNLLEGLNRLQSGFHKQHMRDLFVELCLTVPVRLSSLLPYLPMLMDPLVS *F ALNGSPTLISQGLRTLELCVDNLQPDFLYDHIQPVRAALMQALWKTLRNQDNAALVAFRVLGKFGGGNRKMMVEPQALSY *F IINDKPTISIVTYFQEYETPIDFPVDEAIKSAFRALGSNSTDQFYRRQSWEVIRCFLAAFISLDDEKHMLLKLFTHVDFV *F ENKIMNWSTFQHKAGNETVRETHQTALIGMLVASATKDLRDSVCPVMAAVVRHYTMVAIAQQAGPFPQKGYQATHGIDPM *F ILIDALASCMGHEEKELCKPGIACMGIILDTATNIMGNKDRACKLPIIQYLAEKMVSLCYDRPWYSKVGGCQAIQFLCKH *F MSLRALFQNLFNFLKAFMFVLMDLEGDVSNGAIEITKSYMKSMLEICLTPINECYKNIDLKDLQAKATYEVIHELVRHIT *F SPNTIVREESMVLLKHIGTIQSKTVSEVMDPHKDVLADIIPPKKHLLRHQPANAQIGLMDGNTFCTTLEPRLFTIDLTNT *F YHKLFFHELLTLSEAEDATLAKLDCYKNVPNLIPLRTSALRALAACHYISDIGYKEKIINIIFKVMESDKSELQTTAFHC *F MKHFITGVTLEKEKVQSAMRPLLLKLGDHRNLSIPAIKRLSYFTQIFPQMFNEKLSEQILQHCSKIMEIFVSEYKSTSPN *F VNFFASSKGGEYEQKIVILIEMFFYISASVKYIEKLCQLVLKTEKNLMIEASSPYREALIKFLQRFPTETVDLFLTESLM *F IDPQWNRLFIYLLKHETGVSFRAVIKSSRYNNLIHYLNTHTEFPEALKYEIQHQAVLIIFTLMESDDQWIPTRQDIVDAL *F KNCWQNYLSTLSSEDVLCDLWHLIGKILLHYFSNNTNDIELLFQLLRALCFRFIPDVYFLRDFLQHTVAQSFTVNWKRNA *F FFYFVENFNNSFLSEELKAKIITAVIIPCFAVSFDKGEGNKLIGAPPTPYQEDEKNIVSVFINKVFDPDKQYDDAVRIAL *F LQLACLLVERASQHIHDGDANNKRQGNKLRRLMTFAWPCLLSKSSVDPTARYHGHLLLSHIIARLAIHKKIVLQVFHSLL *F KGHALEARSIVKQALDVLTPAMPLRMEDGNTMLTHWTKKIIVEEGHAMQQLFHILQLIIRHYKVYFPVRHQLVQHLINYM *F QRLGFPPTASIEHKKLAVDLAEVIIKWELHRIKDDRETKTDGTEEELIQESSVKRSGIDLVETRKKSFDIIRETTVQGKC *F VMLLKMAMRPEIWPQPFDIKLNWLDKVLATVETPHHNLNNICTGIDFLTFLTTILSPDQLVSIIRPVQRGLSLCIIHQNT *F RIVRLMHMFLTRIMAIFPPDTQHKHEDLDLLYTAVSKMIAENLTSYEKSPQPNASSLFGTLMILKACTTNNASYIDRILV *F QFIRVLNHLTRDHINTIGGNTVISQSPDSNALPLELLVLSLELIKNRIFVMSVEIRKLFIGTILVSLIEKSTEVKIIKCI *F IKMLDEWIKTKEPNVMTQVPSIREKSALLVKLMQNVEKKFTDEIELNIQFLEIINFIYRDEILKQTELTNKLEGAFLNGL *F RFQNPNVRSKFFEILDSSMRRRLHDRLLYIICSQAWDTIGSHYWIKQCIELLILTANTMMQIQCSNEQFKIPSITSVIPV *F NSSETQENSFVSFLSSHSESFDIIQTVDDKDDVYDIDLNADRKEDCQQILPNRRVTLVELVYKQAEFLEANRNIRTDQML *F VATSQLCHIDTQLAQSVWLSMFPRIWSIFTEDQRCNITKELIPFLSSGTNVNQKDCHPSTLNTFVESLTKCAPPIYIPPN *F LLAYLGKSHNLWHRAILVLEDMAVNQSMQSKDIDGGENQFSDLDVQQSNNIFDSLSKMYSSMHEEDLWAGLWLKFAHYPE *F TNIAVSYEQMGFFEEAQGAYDLAMTKFKQDLSNGVVNTYVNSELLLWENHWMRCAKELNQWDILLDYAQTNKDKNMFLIL *F ESSWRVPDWNLMKIALAKTEQCYLKHYGFKINLYKGYLSILHQEERQTGNIERYVEIASSLCIREWRRLPNIVSHIHLPY *F LQASQQIMELHEASQIHQGLAQSRNNSLHDMKAIVKTWRNRLPIISDDLSHWSDIFTWRQHHYQIITQHLEQQSDQGSTM *F LGVHASAQAIISFGKIARKHNLTGVCQETLSRIYTIPSVPIVDCFQKIRQQVKCYLQMPSTSGKNEINEALEVIESTNLK *F YFTGEMNAEFYALKGLLLAQIGRSEEAGKSFSVAAQLHDGLTKAWAMWGDYMEQIFLKERKITLAVDALICYLQASRNQI *F ESKTRKYIAKVLWFLSYDNNTKILISTLEKHVAGIPPSYWLPWIPQLLCCLEQFEGDVILNLLSQIGRLYPQAVYFPIRT *F LYLTLKIEQREKHKTAEQAVKSSCSNIDGTTLSFGRGASHGNIPSINPIKATPPMWRCSKVMQLQREVHPTILSSLEGIV *F DQMVWFRESWTEEVLRQLRQGLIKCYAIAFEKRDTVQHSTITPHTLHFVKKLGSTFGIGIENVPGSVTSSISNSAASESL *F ARRAQVTFQDPVFQKMKEQFTNDFDFSKPGAMKLHNLISKLKTWIKVLETKVKKLPTSFLIEDKCRFLSNFSQKTAEVEL *F PGELLIPLSSHYYVRIARFMPRVEIVQKNNTAARRLYIRGTNGKIYPYLVVLDSGLGDARREERVLQLKRMLNYYLEKQK *F ETSRRFLNITVPRVVPISPQMRLAEDNPNSISLLKIFKKCCQSMQVDYDMPIVKYYDRLSEVQARGTPTTHTLLREIFSE *F IQWTMVPKTLLKHWALKTFLAATDFWHFRKMLTLQLALAFLCEHALNLTRLNADMMYLHQDSGLMNISYFKFDVNDDKCQ *F LNQHRPVPFRLTPNVGEFITHFGITGPLSAAIVATARCFIQPNYKLSSILQTILRDEIIALQKKGFRECKLIEGSEDRYS *F DGNCMEHSVNIVNSAVDIIMTRFNKISYFDSIENKKISVLVQSATNIDNLCRMDPAWHPWL *F >mei-41.AA *F MYAKSLLEVLQHAHHIGYATHGDIFSGSLHQALLILKECDMDTKLASLNYCHNVLRSQSASSWITNPDVGHYAQLTLEAT *F AIMWSAVAKWLDMGCMTRQELKRLNITTKLLLEVLHMRARPAHHLGYLLLNEILSLPTAIELDDGLLETLSSYIQGQLEH *F SVVPLEQLVHLQQLMLSHWHCHPTHLVPILALMGLKQTEMRSGVVQVLTQSLVEILKKEEVLSKDWQKLIAILRGFKQLE *F KLILSQSQHKIAEHEGHIDSSVLAMLPLQCEIIKVADTNWNNLSMQLVELESKCSADRRHIHLEICSLLMQITFIRHFLK *F TQTQHQLLAILQRHLKLSYLCAIRLETPSSVHTQMQSFYAQQYMRLFQSEETQEIFCSNLPQLYISGFIKPEQLMKALPT *F INNRSGRAQVIRLLLCSQPGKLSVFKVKDRIELYCPKCRPLPKKLPGIYLGKCKQQLPCPDFSSTNLEMIANDLLFYPDF *F ECIAQHLDLLCFEPNVILGLLRETEALQKVSVKVIGQLVSAMRVRSPEFLEQLANLVLAAIKAMLAKPLTEQNVLQQRSM *F LNVLTAIAHMEDNEIWLFHWFKMTFFFLVHTRSLVAQEAVLAATEMCASQGLQTIHLWNWYKRDALDLTVRLALNVYLLD *F GVRFTRSLRALTKMLGFTCVQEFTCKYHRLLTAMVLPHCIVNPLCKGVLVLIAKQMQKHIGTLFSISFLRIYTHVFLTEE *F PELANSCIELVVSCTQSSLQQLMNADVKQTVAELLIYFNRNPTFVMRSFQSLLQLSIGSLEELSSQTANAEFANFIAERF *F LGVITYFESCLSEPSFEKPLKEETLYSLGQIMRFVGSQHVTQFRFKIIAMLSFVHTLQEPRLQRICLKIWHIFLHVVNVQ *F ELGPSLGRIVATLQPLLADNESVKQVNDLYEFIILRNASMLGTFITDLYFLDRMENVSPSIQKCIRRHTAHLDLKGLAEE *F ENQSPPLVDQLRFLQKHITDECLQVRVYALQHLGDLFGRRRPKLNSTILSELPLEPMLEQIVNVLMAGCQHDDSQLQMAS *F AKCLGELGAIDASYLPSNYNFASPQHLPLNILSDDFAVLALTSLCRGYQFQQNTKHVDSFSLAIQETLAICGISPKEQKK *F VQLWQSLPARMRQLMEPMLHSCYTCVHRPSTCLQQPLFGSHYSHNYYEEWAFLWASRLIDYLPSSGRRHLLSSYKPCIKR *F DSNMLSTFYPYILLHALLECTTEQRNHIQEEFMAVLQANEESSSSVRGRQELGAIKENAFKQFESRKYAAGIKPLASTLV *F SDRKEDSSRVPRLAGKLCAELLDFLQRWLREWQRIHGRSTGGKPPETIDSNYRKIHEFLNLIPKLLVSRASYNCGEYARA *F LSYLESYLEEGEDKSQRLLEQFTFLVEVYGSLRDPDSVEGAVQVRSYDMSVTQDILVNRLVERQQDMITSYEQLLSSTDQ *F MQPDHVRAMIDAYLRDTPKTAQLIADGLWQRLSDRYSDQCFAECKSELLWRLGSYDEMEELQSNWPAQCSQGCLKLRRPL *F TTRIEFDSLLDGMRESVLEELRSCSAVQQHSYANAYDAVLKLHLVHELHCSQELVEKLEQDRDEDNQEKLMKNYFDDWQY *F RLQIVQPQVRIQESIYSFRRNILAELQRRLTDRNHLLPHLKTELARIWLNSAQINRNAGQLQRAQLYILKAAEYQPSGLF *F IERAKLLWQKGDQVMAMNYLEEQLSIMRSGCQGNVKQLAAEQRHLFFRGKYLQAVYSAESMHLCADAVLKYFQEAIAVHR *F QSESCHVQMAQFLEKILEARQGGKSEPTGEQDDMLINVMVNYAKSLRYGSEHVYQSMPRLISLWLDTTESSTNTEQVKKM *F NDLLTNCCTALPTAVFYTVYSQMLSRLCHPVNDVFTVLRNVIIKLVEAYPQQSLWMLLPHFKSAKAHRIKRCKLVLTDSR *F LQNSTFQKLLQDFNSLTERLMDLTNKEVTLDRTYKLSDLDTRLSKLCKQPEFSQILLPFEKYMQPTLPLNSDSNSSEGSH *F LPANQSTVNWFPYQQIYISGFQESVLILRSAAKPKKLTIRCSDGKDYDVLVKPKDDLRRDARLMEFNGLVKRYLHQDAPA *F RQRRLHIRTYAVLPFNEECGLVEWLPNLASYRSICMNLYAQRRLVMSTRQLQSLAVPLHESIERKREVFTKQLVPAHPPV *F FQEWLRQRFATPHSWYEARNTYIRTVAVMSMVGYILGLGDRHGENILFAEGNGDAVHVDFNCLFNQGELLPYPEVVPFRL *F THNMIVAMGPLGVEGSFRKCCEITLRLLKQESKTLMSILRPFVYDVGAQTRKGAATAKITKDVQRIADRLQGHVKRQQAN *F SIPLSTEGQVNFLINEATKVDNLASMYIGWGAFL *F >PKA-C3.AA *F MSTATCARFCTPLSSGTAGSTSKLTTGNGSGNTMTSAYKIPSNNSTTANDSSNTETTFTFKLGRSNGRSSSNVASSESSD *F PLESDYSEEDPEQEQQRPDPATNSRSSSTATTTTTSSADHDNDVDEEDEEDDENEGEGNGRDADDATHDSSESIEEDDGN *F ETDDEEDDDESEESSSVQTAKGVRKYHLDDYQIIKTVGTGTFGRVCLCRDRISEKYCAMKILAMTEVIRLKQIEHVKNER *F NILREIRHPFVISLEWSTKDDSNLYMIFDYVCGGELFTYLRNAGKFTSQTSNFYAAEIVSALEYLHSLQIVYRDLKPENL *F LINRDGHLKITDFGFAKKLRDRTWTLCGTPEYIAPEIIQSKGHNKAVDWWALGVLIYEMLVGYPPFYDEQPFGIYEKILS *F GKIEWERHMDPIAKDLIKKLLVNDRTKRLGNMKNGADDVKRHRWFKHLNWNDVYSKKLKPPILPDVHHDGDTKNFDDYPE *F KDWKPAKAVDQRDLQYFNDF *F >CG12069.AA *F MGPQPEQAQMHFSPKVDYILILDKLREDFNKKFATNTPSPSTGLDDYEIKATLGSGSFGKVQLVRERESGVYYASKQLSK *F DQIVKTKQVSHVMSEKNVLRSMTFPNTVNLIASYKDFDSLYLVLPLIGGGELFTYHRKVRKFTEKQARFYAAQVFLALEY *F LHHCSLLYRDLKPENIMMDKNGYLKVTDFGFAKKVETRTMTLCGTPEYLPPEIIQSKPYGTSVDWWAFGVLVFEFVAGHS *F PFSAHNRDVMSMYNKICEADYKMPSYFSGALRHLVDHLLQVDLSKRFGNLINGNRDIKEHEWFKDVEWIPLLNQTVNAPY *F VPNISNPEDISNFDKVSDKPRPKAKTMRHEEAFADF *F >Pka-C2.AA *F MSQHTSQYVFNSKEDYNVILDNMSREFEERWNHQTQSPYTNLENYITRAVLGNGSFGTVMLVREKSGKNYYAAKMMSKED *F LVRLKQVAHVHNEKHVLNAARFPFLIYLVDSTKCFDYLYLILPLVNGGELFSYHRRVRKFNEKHARFYAAQVALALEYMH *F KMHLMYRDLKPENILLDQRGYIKITDFGFTKRVDGRTSTLCGTPEYLAPEIVQLRPYNKSVDWWAFGILVYEFVAGRSPF *F AIHNRDVILMYSKICICDYKMPSYFTSQLRSLVESLMQVDTSKRLGNSNDGSSDVKSHPWFQGVDWFGILNQEVTAPYQP *F TISGAEDLSNFENFEFKDRYKSRINRHPELFANF *F >Pka-C1.AA *F MGNNATTSNKKVDAAETVKEFLEQAKEEFEDKWRRNPTNTAALDDFERIKTLGTGSFGRVMIVQHKPTKDYYAMKILDKQ *F KVVKLKQVEHTLNEKRILQAIQFPFLVSLRYHFKDNSNLYMVLEYVPGGEMFSHLRKVGRFSEPHSRFYAAQIVLAFEYL *F HYLDLIYRDLKPENLLIDSQGYLKVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLVYEMAAGYPP *F FFADQPIQIYEKIVSGKVRFPSHFGSDLKDLLRNLLQVDLTKRYGNLKAGVNDIKNQKWFASTDWIAIFQKKIEAPFIPR *F CKGPGDTSNFDDYEEEALRISSTEKCAKEFAEF *F >Pk61C.AA *F MAKEKASATVSLGESNFRDINLKDLAVVVEAASRLHHQQNVCGCGAVSSTENNNNSRYGSSKYLTNGHTSPLAAAVASNS *F SSVATTPHCRMLHNCSLQQYQNDIRQQTEILDMLRQQHQQGYQSQQQQQQPQQQQEQQQQQEQSQQQQQLQNPAPRRSPN *F DFIFGRYIGEGSYSIVYLAVDIHSRREYAIKVCEKRLILRERKQDYIKREREVMHQMTNVPGFVNLSCTFQDQRSLYFVM *F TYARKGDMLPYINRVGSFDVACTRHYAAELLLACEHMHRRNVVHRDLKPENILLDEDMHTLIADFGSAKVMTAHERALAT *F EHCSEQRRSNSDEDDEDSDRLENEDEDFYDRDSEELDDGDDEQQQEEMDSPRHRQRRYNRHRKASFVGTAQYVSPEVLQN *F GPITPAADLWALGCIVYQMIAGLPPFRGSNDYVIFKEILDCAVDFPQGFDKDAEDLVRKLLRVDPRDRLGAQDEFGYYES *F IRAHPFFAGIDWQTLRQQTPPPIYPYLPGVSQDEDFRSSYTVPGDLEPGLDERQISRLLSAELGVGSSVAMPVKRSTAKN *F SFDLNDAEKLQRLEQQKTDKWHVFADGEVILKKGFVNKRKGLFARKRMLLLTTGPRLIYIDPVQMIKKGEIPWSPDLRAE *F YKNFKIFFVHTPNRTYYLDDPEGYAIHWSEAIENMRKLAYGDPSSTSAVSCSSGSSNSLAVISNSSAASSSNSPTVKRSS *F PVNAPQASTASDNRTLGSTRTGTSPSKKTASK *F >aPKC.AA *F MPSQILNDGSSVSLNSASMNMANTPNSITVKTAYNGQIIITTINKNISYEELCYEIRNICRFPLDQPFTIKWVDEENDPC *F TISTKMELDEAIRLYEMNFDSQLVIHVFPNVPQAPGLSCDGEDRSIYRRGARRWRKLYRVNGHIFQAKRFNRRAFCAYCQ *F DRIWGLGRQGFKCIQCKLLVHKKCHKLVQKHCTDQPEPLVKERAEESSDPIPVPLPPLPYEAMSGGAEACETHDHAHIVA *F PPPPEDPLEPGTQRQYSLNDFELIRVIGRGSYAKVLMVELRRTRRIYAMKVIKKALVTDDEDIDWVQTEKHVFETASNHP *F FLVGLHSCFQTPSRLFFVIEFVRGGDLMYHMQRQRRLPEEHARFYAAEISLALNFLHEKGIIYRDLKLDNVLLDHEGHIK *F LTDYGMCKEGIRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLLYEMLAGRSPFDLAGASENPDQNTEDYLFQV *F ILEKTIRIPRSLSVRAASVLKGFLNKNPADRLGCHRESAFMDIVSHPFFKNMDWELLERKQVTPPFKPRLDSDRDLANFP *F PEFTGEAVQLTPDDDHVIDNIDQSEFEGFEYVNPLLMSLEDCLYGEDSSDYDSVADDLSLLHLNSAAGAGGSVSSNNNLS *F QQQHYRQQQQQQYQPHSAYSQQQQQQQQHQHAAATSAASTSAATAATTSS *F >Pkc98E.AA *F MFTGKLQIKVCEASGLRPTDFQKRHNLTFGKLADEQLIDPYVSIDVDESHFDRATTRPKTFDPVWNEQFVHDVTNVSNIN *F LTVFHDAALPPDDFVANCIISFEDLMQSETAVQDLWVNLEPQGKIHVIIELKNRTDKAKAEAVVEHTVAVNKEFKERAGF *F NRRRGAMRRRVHQVNGHKFMATFLRQPTFCSHCREFIWGIGKQGYQCQVCTLVVHKKCHLSVVSKCPGMRDEQPAKVEMV *F PAGQRFNVNLPHRFVVHSYKRFTFCDHCGSLLYGLIKQGLQCETCGMNVHKRCQKNVANTCGINTKQMAEILSSLGISPD *F KQQPRRSKYLNQQGGEDNYGASLGADGDGAPGQSFRSCALSVDSLATSTTTMTSGYNSSSCMSLAVTGSGGVGATGETRP *F GKCSLLDFNFIKVLGKGSFGKVMLAEKKGTDEIYAIKVLKKDAIIQDDDVDCTMTEKRILALAANHPFLTALHSCFQTPD *F RLFFVMEYVNGGDLMFQIQKARRFEASRAAFYAAEVTLALQFLHTHGVIYRDLKLDNILLDQEGHCKLADFGMCKEGIMN *F GMLTTTFCGTPDYIAPEILKEQEYGASVDWWALGVLMYEMMAGQPPFEADNEDELFDSIMHDDVLYPVWLSREAVSILKG *F FLTKNPEQRLGCTGDENEIRKHPFFAKLDWKELEKRNIKPPFRPKMKNPRDANNFDAEFTKEDPVLTPIGNEVVRCINQD *F EFAGFSFVNPKFGPERKVY *F >inaC.AA *F MAAAAVATPGATVLPPSVPSAAPGAKAPAAGAGKGPGNLLEITGEANIVNYMKNRLRKGAMKRKGLEMVNGHRFGVRFFK *F NPTYCGHCKDFIWGFGKQGFQCEECRFNIHQKCCKFVVFKCPGKDTDFDADCAKVKHGWISTTYTTPTFCDECGLLLHGV *F AHQGVKCENCNLNVHHACQETVPPMCGADISEVRGKLLLYVELKGNNLKVDIKEAANLIPMDTNGFSDPYIAVQMHPDRS *F GRTKKKTKTIQKNLNPVFNETFTFELQPQDRDKRLLIEVWDWDRTSRNDFMGSFSFSLEELQKEPVDGWYKFLSQVEGEH *F YNIPCVDAFNDIARLRDEVRHDRRPNEKRRMDNKDMPHNMSKRDMIRAADFNFVKVIGKGSFGKVLLAERRGTDELYAVK *F VLRKDVIIQTDDMELPMNEKKILALSGRPPFLVSMHSCFQTMDRLFFVMEYCKGGDLMYHMQQYGRFKESVAIFYAVEVA *F IALFFLHERDIIYRDLKLDNILLDGEGHVKLVDFGLSKEGVTERQTTRTFCGTPNYMAPEIVSYDPYSIAADWWSFGVLL *F FEFMAGQAPFEGDDETTVFRNIKDKKAVFPKHFSVEAMDIITSFLTKKPNNRLGAGRYARQEITTHPFFRNVDWDKAEAC *F EMEPPIKPMIKHRKDISNFDDAFTKEKTDLTPTDKLFMMNLDQNDFIGFSFMNPEFITII *F >PKCdelta.AA *F NRRGAIKHQKTHDINGHRFVAKFFRQPTFCAFCNLFLWGFGKQGYQCIICQTVVHKKCHDKLLGKCSGSVFTSASTILLR *F ERFKIDMPHRFKPHTFMSPTFCDHCGSLMGGFFIQGLKCEECDVNCHKKCERLTANLCGVNQKLIVEALNHVKRETSYTY *F SQFQKSGRFTAPATVIPRFKNYSVDDFHFLAVLGKGSFGKVLLAELRDTTYYYAIKCLKKDVVLEDDDVDSTLIERKVLA *F LGTKHPYLCHLFCTFQTESHLFFVMEYLNGGDLMFHIQESGRFSEERARFYGAEIISGLKFLHKKGIIYRDLKLDNVLLD *F YEGHVRIADFGMCKLQIYLDKTADSFCGTPDYMAPEIIKGEKYNQNVDWWSFGVLLYEMLIGQSPFSGCDEDELFWSICN *F EIPWFPVYISAEATGILKGLLEKDYTKRIGSQYSPAGDIADHIFFRPIDWGLLEKRQIEPPFKPQVKHPLDTQYFDRVFT *F RERVRLTPIDKEILASMDQKQFHGFTYTNPHITLD *F >Pkc53E.AA *F MSEGSDNNGDPQQQGAEGEAVGENKMKSRLRKGALKKKNVFNVKDHCFIARFFKQPTFCSHCKDFIWGFGKQGFQCQVCS *F YVVHKRCHEYVTFICPGKDKGIDSDSPKTQHNFEPFTYAGPTFCDHCGSLLYGIYHQGLKCSACDMNVHARCKENVPSLC *F GCDHTERRGRIYLEINVKENLLTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDL *F KPEDKDRRILIEVWDWDRTSRNDFMGALSFGISEIIKNPTNGWFKLLTQDEGEYYNVPCADDEQDLLKLKQKPSQKKPMV *F MRSDTNTHTSSKKDMIRATDFNFIKVLGKGSFGKVLLAERKGSEELYAIKILKKDVIIQDDDVECTMIEKRVLALGEKPP *F FLVQLHSCFQTMDRLFFVMEYVNGGDLMFQIQQFGKFKEPVAVFYAAEIAAGLFFLHTKGILYRDLKLDNVLLDADGHVK *F IADFGMCKENIVGDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGEDEEELFAAITDHNVSYP *F KSLSKEAKEACKGFLTKQPNKRLGCGSSGEEDVRLHPFSRRIDWEKIENREVQPPFKPKIKHRKMCPTLTSSSHQRKQT *F >CG7125.AA *F MEGPEVTFLFQFGSVRDAVCVPANSLTLKTLKDLACDFINTKIPDSGLTYLSERILLFVHDYSTPNVLHIINSAAEVVDE *F TLVEIVLTASPILYPTSEMPTLKPHSLNVHSYKGPTFCDFCGEMLFGLVRQGLKCDGCGQNYHKRCVVKIPNNCNRSNDA *F TSRRSFTLQAPRSPSGSSQQSLVSTEDSTGRNDSSSSLNIPGRHQRTHSSGSRNGLMVLRIPHTFMVHTYGIPTVCQLCK *F KLLKGLFKQGLQCRDCQYNTHKKCMDKVPHDCTGEAQLSQLQIQAGAKERDNFFREEFDDSDGDDGSEEAPKALTNAHNS *F PPELVNGGLVDDSSVSGGETSKSSSDGQSEQSQSSTSSSPSANIPLMRIVQSVKHTKKRGGQALKEGWLVHYTSLDKAVK *F RYYWRLDSKTITLFVSEQGSKYHKELPLAELLSIESHLGDPRPECNYCFELRLPNLSYFVGQDPQVGAKEEQAVRLPPPD *F SGIGSDIAKSWETSIRQAFMHVTNTQCCESEEQVQDMGQLYQIFPDEVLGSGQFGVVYGGVHKKTQREVAIKVIDKLRFP *F TKQEAQLKNEVAILQNISHCGVVNLERMFETPERIFVVMEKLKGDMLEMILSHARGRLSERVTKFLITQILIALKYLHSQ *F NIVHCDLKPENVLLSSDAEFPQVKLCDFGYARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGT *F FPFNEEEDINDQIQNAAFMYPPNPWKEISSNAIDLINNLLQVKQRKRYTVDKSLLHYWLQDKQTYRDLRNLEAQVGAGRY *F LTHEADDLRWD *F >Pkg21D.AA *F MAAGMLTDREREAIVSNLTKDVQALREMVRSRESELVKLHREIHKLKSVLQQTTNNLNVTRNEKAKKKLYSLPEQCGEQE *F SRNQNPHLCSSCGMVLPTSPEFALEALSLGPLSPLASTSSASPSGRTSADEVRPKAMPAAIKKQGVSAESCVQSMQQSYS *F IPIPKYEKDFSDKQQIKDAIMDNDFLKNIDASQVRELVDSMYSKSIAAGEFVIREGEVGAHLYVSAAGEFAVMQQGKVLD *F KMGAGKAFGELAILYNCTRTASIRVLSEAARVWVLDRRVFQQIMMCTGLQRIENSVNFLRSVPLLMNLSEELLAKIADVL *F ELEFYAAGTYIIRQGTAGDSFFLISQGNVRVTQKLTPTSPEETELRTLSRGDYFGEQALINEDKRTANIIALSPGVECLT *F LDRDSFKRLIGDLCELKEKDYGDESRKLAMKQAQESCRDEPKEQLQQEFPDLKLTDLEVVSTLGIGGFGRVELVKAHHQD *F RVDIFALKCLKKRHIVDTKQEEHIFSERHIMLSSRSPFICRLYRTFRDEKYVYMLLEACMGGEIWTMLRDRGSFEDNAAQ *F FIIGCVLQAFEYLHARGIIYRDLKPENLMLDERGYVKIVDFGFAKQIGTSSKTWTFCGTPEYVAPEIILNKGHDRAVDYW *F ALGILIHELLNGTPPFSAPDPMQTYNLILKGIDMIAFPKHISRWAVQLIKRLCRDVPSERLGYQTGGIQDIKKHKWFLGF *F DWDGLASQLLIPPFVRPIAHPTDVRYFDRFPCDLNEPPDELSGWDADF *F >CG4839.AA *F MACFPRLFHNRSKNKFTPAEDENTDTILNTQDSNVQIGKYIRREEKPLYSPIVTPSASEAKLQRLRQQQQPPGSPPAPAR *F WTLHSVAENGSANNGVQERKFRTNKDKLQAQDACPSGGSAINVIRSSSKLKPVKERRPTALPTEVPSIEIEDVRDAKDTG *F DGMDSLGERVQPGANAHVDVVDGGGKSKLKVNVNGIIDESVAHDDDYDTPSSNSIATTPVTEGPVKFFIGHTQELSAQQT *F DDTLSCQSSDANNNSAAKLTNGVVPQEQPEREMGKQQALQAPPRAKSSSNVSLNYLAKSQTDSQRRRRVSTMPDINIPPA *F PPLPPELLVPGTPLHLRKKRSMERQQQQKQQEQQQQQEIQQDATNSQDQMAGAEESHKSSDSSTPKTHRVEGGLIYVRPN *F FPIQSDIEIESIAKDEGTRNLIRTAIERNDFLNNLMDKERKEMVINAMAPASYRKHSLIIREHEEGSEIYVSAEGQYDVI *F RGGQLVASFGPATVFGELAILYNAPRQASIEAATDARVWKIARETFRAIMQISGSREREENLQFLRSAPFLQEFDQSLLL *F KVVDLLQRKFYETDSCIVREGELGNEFYIIRCGTVTIKKLDEQQQEQIVANRKRGDYFGEQALLNADVRQASVYADAPGT *F EVLMLDREAFISYLGTIKQLREKPSSQRNDTSGRSSTKSLEFDNEYSQVAISELKKIATLGCGAFGRVDLVAYNQQALAL *F KIIKKIEVVKQDQIEHVYNEKNVMIKCRQSPFIVQLYRTYRNDKYVYFLMEACMGGDVWTVMSKRQYFDEKTAKFIAGCV *F VEAFDYLHSHHFIYRDLKPENLMLGTDGYCKLVDFGFAKFVRQNEKTNTFAGTPEYVAPEIILDRGHDRAVDYWALGILV *F YELLVGKTPFRGVNQIKIYQQILSGIDVIHMPSRIPKSAQHLVRHLCKQLPAERLGYQRKGIADIKRHSWFESLDWQRLK *F LKQLPSPIKRPLKSWTDLQYFGPSGVENDYEPPEELSGWDKDF *F >for.AA *F MKIKHYPGKAVDASLSLEGSSAMGALYEANWLRAANQPAAPATTGTKLSRQSSSAGSSFLIEGISALSKYQMTLENIRQL *F ELQSRDKRIASTIKELSGYRPSALQHHQQQQMHNVWVAEDQDQEHEELEDASEGKEKLASIQEPPAVNHYVLDPTERPRV *F PRPRQQFSVKPPSLRRSQTMSQPPSYATLRSPPKIKENLSKSSSAYSTFSSAAEDSQDQVVICQQPQRLMAPPPREPPPE *F PPKRVSKPLSRSQTSVQRYATVRMPNQTTSFSRSVVRSRDSTASQRRLSLEQAIEGLKLEGEKAVRQKSPQISPAASSNG *F SSKDLNGEGFCIPRPRLIVPVHTYARRRRTGNLKEQSSGGQEEEAEKGKGWKDFYVLSQDRHSSFYINRIGQNYDYDYPI *F NFNIFSPDGRVEVSREGKYLSTLSGAKVLGELAILYNCQRTATITAITECNLWAIERQCFQTIMMRTGLIRQAEYSDFLK *F SVPIFKDLAEDTLIKISDVLEETHYQRGDYIVRQGARGDTFFIISKGKVRVTIKQQDTQEEKFIRMLGKGDFFGEKALQG *F DDLRTANIICESADGVSCLVIDRETFNQLISNLDEIKHRYDDEGAMERRKINEEFRDINLTDLRVIATLGVGGFGRVELV *F QTNGDSSRSFALKQMKKSQIVETRQQQHIMSEKEIMGEANCQFIVKLFKTFKDKKYLYMLMESCLGGELWTILRDKGNFD *F DSTTRFYTACVVEAFDYLHSRNIIYRDLKPENLLLNERGYVKLVDFGFAKKLQTGRKTWTFCGTPEYVAPEVILNRGHDI *F SADYWSLGVLMFELLTGTPPFTGSDPMRTYNIILKGIDAIEFPRNITRNASNLIKKLCRDNPAERLGYQRGGISEIQKHK *F WFDGFYWWGLQNCTLEPPIKPAVKSVVDTTNFDDYPPDPEGPPPDDVTGWDKDF *F >CG2049.AA *F MIVPTFHALGQVLRQMFAVQLQQQQQRTHNQLHYLDLSPARTINQLLVCPCASNPVPPAARISLVHITLEPINASRTTSC *F LIEEVAEPDSQPEIKPVAEAQSAKVSEACVESILPETVEKLETADQVQQVIPQLGKLYVGSSQQQYAQQSSPIIQEPATP *F TIYGNSAAAGAPQFPQPAQRQEKQPPQQQPIYANQYELNVAKAAAAASVYSPSSSTTSNSNQQQQQQRRNVARGLQYRES *F GGLETGRAGKQPPNAGMLSMDNFRLLSVLGRGHFGKVILSQLRSNNQYYAIKALKKGDIIARDEVESLLSEKRIFEVANA *F MRHPFLVNLYSCFQTEQHVCFVMEYAAGGDLMMHIHTDVFLEPRAVFYAACVVLGLQYLHENKIIYRDLKLDNLLLDTEG *F YVKIADFGLCKEGMGFGDRTGTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIFEMLVGESPFPGDDEEEVFDSIVNDEV *F RYPRFLSLEAIAVMRRLLRKNPERRLGSSERDAEDVKKQAFFRSIVWDDLLLRKVKPPFVPTINHLEDVSNFDEEFTSEK *F AQLTPPKEPRHLTEEEQLLFQDFSYTAEWC *F >SAK.AA *F MLSNRAFGETIEDYEVQHLLGKGGFATVYKARCLHTHQDVAIKMIDKKLIQGTGLTNRVRQEVEIHSRLKHPSVLQLYTF *F FQDANYVYLVLELAHNGELHRYMNHIARPFTETEAASILKQVVAGLLYLHSHNIMHRDISLSNLLLSREMHVKIADFGLA *F TQLKRPDERHMTMCGTPNYISPEVVSRTSHGLPADVWSVGCMLYTLLVGRPPFETDAVQSTLNKVVMSEYIMPAHLSYEA *F QDLINKLLKKLPHERITLEAVLCHPFMLKCSNGGHSAPGALNVFSQSMESGDSGIITFASSDSRNSQQIRSVENSGPQQV *F LPQIREEFKQVHHKLPYEQTGLFGQASTGLAEPNWPGAAKSSAFCMEAGNVPNSKQASLKEDRISVPPLNTKRLLPTRYK *F TKNAIMSILRNGEVVLEFLKFRPTYNEDRINDICRISDDGQRIIIYQPDPGRGLPVREQPPDLQIPSGDCVYNYDNLPSK *F HWKKYIYGARFVGLVKSKTPKVTYFSTLGKCQLMETMTDFEIRFYSGAKLLKTPSEGLKVYDRNGMLLSDYSCSESRSLI *F EHGNECFTHCVNISNALEVAQTKDNSCFPVTIGRRPITDVQPAQRLDGLRDTTNIAFSTPKSNQGSINFSLSTISSTRNT *F SDFGTNCSRSNMLAAHQNIPIKRINVPEIGIATELSHGVVQVQFYDGSVVSVIPSMQGGGITYTQPNGTSTHFGKGDDLP *F FPVRDRVGQIPNIQLKLKTAPLLGSGRKTDYNNAMTPKTTTPYYNRMLL *F >polo.AA *F MAAKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQKEKTAQEITIHRSL *F NHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLL *F HVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEY *F RVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLPSSCLTMAPRIGSNDTIEDSMHRKPLMEMNGIRP *F DDTRLESTFLKANLHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPRILQGNLGDENTDPAAQPLFWISKWVDYSD *F KYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKMKLLSYFKRYMIEHLVKAGANNVN *F IESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLNFSDHMKLILCPRMSAITYMDQEKNFRTYRFSTIVENGVSKDLYQKI *F RYAQEKLRKMLEKMFT *F >lok.AA *F MARDTQGTQGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKV *F HFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVN *F RKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVL *F EFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVM *F RTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDEYGTPAAQQIKKGRFAYGHPSWKSVSQRAKLL *F INQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLMKLDGMEIEEENFLEPPTKRSRR *F >ksr.AA *F MSSNNNAPASAPDTGSTNANDPISGSLSVDSNLVIIQDMIDLSANHLEGLRTQCAISSTLTQQEIRCLESKLVRYFSELL *F LAKMRLNERIPANGLVPHTTGNELRQWLRVVGLSQGTLTACLARLTTLEQSLRLSDEEIRQLLADSPSQREEEELRRLTR *F AMQNLRKCMESLESGTAASNNDPEQWHWDSWDRPTHIHRGSVGNIGLGNNSTASPRTHHRQHGVKGKNSALANSTNFKSG *F RQSPSATEELNSTQGSQLTLTLTPSPPNSPFTPSSGLSSSLNGTPQRSRGTPPPARKHQTLLSQSHVQVDGEQLARNRLP *F TDPSPDSHSSTSSDIFVDPNTNASSGGSSSNVLMVPCSPGVGHVGMGHAIKHRFTKALGFMATCTLCQKQVFHRWMKCTD *F CKYICHKSCAPHVPPSCGLPREYVDEFRHIKEQGGYASLPHVHGAAKGSPLVKKSTLGKPLHQQHGDSSSPSSSCTSSTP *F SSPALFQQRERELDQAGSSSSANLLPTPSLGKHQPSQFNFPNVTVTSSGGSGGVSLISNEPVPEQFPTAPATANGGLDSL *F VSSSNGHMSSLIGSQTSNASTAATLTGSLVNSTTTTSTCSFFPRKLSTAGVDKRTPFTSEYTDTHKSNDSDKTVSLSGSA *F STDSDRTPVRVDSTEDGDSGQWRQNSISLKEWDIPYGDLLLLERIGQGRFGTVHRALWHGDVAVKLLNEDYLQDEHMLET *F FRSEVANFKNTRHENLVLFMGACMNPPYLAIVTSLCKGNTLYTYIHQRREKFAMNRTLLIAQQIAQGMGYLHAREIIHKD *F LRTKNIFIENGKVIITDFGLFSSTKLLYCDMGLGVPHNWLCYLAPELIRALQPEKPRGECLEFTPYSDVYSFGTVWYELI *F CGEFTFKDQPAESIIWQVGRGMKQSLANLQSGRDVKDLLMLCWTYEKEHRPQFARLLSLLEHLPKKRLARSPSHPVNLSR *F SAESVF *F >phl.AA *F MSSESSTEGDSDLYDPLAEELHNVQLVKHVTRENIDALNAKFANLQEPPAMYLIEYQELTSKLHELEAKEQELMERLNSQ *F DQQEDSSLVERFKEQPHYQNQTQILQQQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQPKILLRAHLPNQQRTSVEVISG *F VRLCDALMKALKLRQLTPDMCEVSTTHSGRHIIPWHTDIGTLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCR *F RLLFTGFYCSQCNFRFHQRCANRVPMLCQPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSS *F KPPSSSSGNHRQGRPPRISQDDRSNSAPNVCINNIRSVTSEVQRSLIMQARPPLPHPCTDHSNSTQASPTSTLKHNRPRA *F RSADESNKNLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKT *F RHCNILLFMGCVSKPSLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDL *F SVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFM *F VGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPNLTQSQLQNDEFLYLPS *F PKTPVNFNNFQFFGSAGNI *F >CG4588.AA *F MNRYITLTQLGDGTYGTVVLGQRKDTGEKVAIKRMKRKYYSWEEAMNLREVKSLKKLSHPNIVKLKEVIRENDTLYFVFE *F YMKENLYQMIKDRDTHLPEPELKSILFQVLTGLAFMHRHGFFHRDLKPENLLCSGPDLIKIADFGLAREIRSRPPFTDYV *F STRWYRAPEVLLHSTNYGSTIDLWAMGCIMAELYTFRPLFPGSSEVDQLFKICSVLGTPDKDDWPDGYRLASMIHFRYPD *F CIKVPLSSVVSRCSQNGLDLLEDMLAYDPDKRPTAQQSLKYPYFHALKRISPTAATKANVRLNSKYAASNGHPVQSVSNN *F VLPVQEKLQAVTELLHQTNNNMNSHSNLGKNNNLAPKNGGVPRKYQPKLSFLTTSEMGAGSHADSSSLVGGATVDGVPVP *F QHQNGGESINDIYLNRNISQLFGLPAGPQHQQQQQVHHPNVSFSTTSSRNAGAIYVNGSHLSYDTANMNAKNNAKFVVGA *F SNGGYYVPVARPSLLGPDAKVYNVFSKVSASQAPPSLIVRQPQLQPEVAPAPMRLSGRSRAAAAVQDPKMGALKSDDLDL *F ILGSKLKTSAKRQRNAKANILLEDLFGQLSMDSDSDGKYPHTVPPTQQAKSGKTSTGGHGAGARERDLFGESYLPRPGLR *F KKATQSSLEVNNGSHADSLWWQVQGFGFISPTGEVYMVYGLHKAWVSERGIRFLSYHKLVTATMGQDSSYKSGLSQALTH *F QSSALHRFSYNSNLFAMNVLAGIIASHPDELIASPAQYEFHYSVHDSHSGDVKDQFEHRRGEYVTGRYSLVDPDGHRRIV *F DYTADPLLGFNAQVRREPISRY *F >Ret.AA *F MESTTIVFVTLLTIITQRKHCAAVDVYFPTTSVKFNLPINEESESIFSKIPLAQFQVLRMEDNRLASDYLYSLEQNPLLR *F INSSSGEIYMRTDYRSPNSSATFLVTAFPRDQPDHELLNVSHLSLEVTPQPLEEYCSELEHICFWSSAQYTIAESHGPYR *F RKDFFEPVLIGALNSRAAKYLCPHVSLEYSLNAGSSHFVLKQNRLYTRQTLDHDELNGLNAKAGQLQARITCTVKLSSRD *F QRKFSRILDIKLLDRNDNGPKLQESSSKFDFYLEQPYFQADEEAGKKVIYVDKDTLEANAHLVYAVHNDSHGLFRPDCHA *F YEADHTGRPHTIVSCQLRFSRNGVFRETPYCVSLEARDLTIVSRVDAMSATANVCYHINLSKLHESEQELPQALPLRARQ *F HRIFESEEFNGDSAGRSLSPPTVDYDKDVSVYRSAASNFRVVQPDSFLDLMRLRSIRFDIVEDKLGAFGITSTSGIVFVK *F NPQVLEEAPETIYFLNVTWIDQQRLSHVRVINVHLVHGRPENTSCELKVKSRSQTCAQIKYQSQCVRYCGLATGGGSCQW *F RGSNSAMFGTRYGSCVPESRYCPDHVCDPLEELNPMACPQDCTPAGRIVGPHSSNENKRGIYSASGTCICEDNGKCSCAP *F LDEEPKMKKPRKRKNETEAEPLLGVRRGTPPNQPLQDPMLLGVLNVAGFECDRSCMFFVITCPLLFVLLLLCLLIAQRKM *F LQRRLGKQSMTTSSKQALPESGGGDFALMPLQSGFRFESGDAKWEFPREKLQLDTVLGEGEFGQVLKGFATEIAGLPGIT *F TVAVKMLKKGSNSVEYMALLSEFQLLQEVSHPNVIKLLGACTSSEAPLLIIEYARYGSLRSYLRLSRKIECAGVDFADGV *F EPVNVKMVLTFAWQICKGMAYLSELKLVHRDLAARNVLLADGKICKISDFGLTRDVYEDDAYLKRSRDRVPVKWMAPESL *F ADHVYTSKSDVWSFGVLCWELITLGASPYPGIAPQNLWSLLKTGYRMDRPENCSEAVYSIVRTCWADEPNGRPSFKFLAS *F EFEKLLGNNAKYIDLETNAVSNPLYCGDDSALITTELGEPESLQHLWSPPKIAYDIHDQATSYDQSEEEMPVTSTAPPGY *F DLPRPLLDATANGQVLRYENDLRFPLNIRKSSCTPSYSNMTSEPPATTSLPHYSVPVKRGRSYLDMTNKSLIPDNLDSRE *F FEKHLSKTISFRFSSLLNLSETKEVSPGWQAEDAV *F >CG3216.AA *F MHLLGISIHFFFLMYVNCFSAHPNPRRNDITWDDLNKDISLDSTTSLAGLNASDAGLEQRMYERSRESKSTQLSRYTEVG *F EMGSTMRVYNVGVLMASHLDSPFDLERCGPAVDLALDEINKVFLKPHNITLLKKKGSYPSCSGARAPGLAADMYFQDDVI *F AFIGPACAFALEPVARLAAYWNKPIITGMGDQPPSSEGELTVTSGILGRIHKWKNENTGMFKDKSKYPTLTRMSYCQCRL *F ILVFASVIRQFNWNHVALLVDRSELFSWTVGKNLEYGLRQEGLLSFVKELNGNEEEVYENYLKDASMYARVVILSVRGVL *F VRKFMLAAHSLGMTNGEWVFLDVEIFQSEYWGDKGWEMKDEHDAKARKAYEALLRVSLLQPTSPKFQDFADNVRENALYD *F YNYTFGEGEEVNFFIGAFYDGVYLLGMALNETLTEGGDIRDGVNITRRMWNRTFEGITGHVRIDDNGDRDADYSILDLDP *F INGKFSVVAHYSGVHKVYSAVHGKKIHWPGGREEPPPDVPPCGFLGNSTDCVGNFTPRSINILFIYTGTDFRQMKLSKEL *F NNMSWRVRPDDVLIEMGGMFGSKGGLQRLDVENISLQQFGIHSGRASIASFTSLPPQVYTTIGQFKGERVAIKKVNVKKV *F DLTPQLLWEIKQARDVSHENTVRFVGACIDLPRPTVLILTEYCSRGSLKDVLENEAIELDWNFRMSLIHDIVKGMNYLHN *F SDVAAHGKLRSCNCLIDGRFVLKISDFGLRTLTTPSDFVRDQNYYLKLLWIAPELLPLTTIPGCCPATQRGDVYSFGIIL *F EEIVNRGGPYQEARQQMDVHTILHKVRQCNGFRPLIRERECPPDLLELMEKCWADNQEERPTFSTIRSNIRTIMKGFCEN *F LMDDLLNRMEQYANNLESLVEEKTRQLSLEKQRTEELLYQILPRPVAQQLMAGDLVEPEEFSSVTIYFSDIVGFTELCAR *F SSPMDVVNFLNDLYSTFDRIIGFYDVYKVETIGDAYLVVSGLPEPNGDKHAREIALMALDILRAVSSFNLRHKPEYKIQI *F RIGMHSGSVCAGVVGKKMPHYCLFGDTVNTASRMESTGQPGKIHVSSATKAILDKFGTFQMEQRGDVELKGKGTVTTYWL *F NSTSEGEARPPTPQILTTDEVPFPLLFAGMGK *F >CG4224.AA *F MLLVLLLVGLVFGPKDCVATCREEPARDCEAICDANGRNCTIRALVLLPDDNMYQASLPRVLPILKVAEQQIRSKSLIPS *F HIDFEWLAHDTKCDASLGVIKAMDGIIKQCAQVIFGPVCDYSLVFGLPCEYVLAPISRYADVWQVPVLTTGGNAKEFNKK *F SESYSTLTRLKGAQVNNLGNVVRAILNSFNWTRTALIYQNENAKVKGNSVCFLCLAAIHDTIEEHSVYQLGFDTSTWTKA *F DITRMLKNVAMQSRIVIMCADPQSIRQIMLTAEELNMIDSGEYVFINIELFSRVQYLTSQPWYDKNDTDLNNERAQKAYT *F AMLTVTPKQPNDNEYTRVSNEIKAIAAEKYNYTFSDNEPISAFVTSFFDGVLLYANALNESIREDPTMLTRPINGTDMVR *F RMWNRSFTGITGNVTIDANGDRLSAYSLLDMNPTTGRFEIVAHFLHNRLEFEANKEIHWAGDREEAPPDRPICGYDGALC *F PDNSLPGYAILSIVLGTMVVVMAVCFFFGYRHYIAEAEINSMSWKVSLEDVMFRDAAERGLRGSFHSLVKQSSQLTLMSE *F DMVSINGDRQIFIPVGMFRKSKVAIKPVEVDNVQGLLTRSLMLELKRMKDLQHDHLVKFYGACLDQRRSFLLTEYCPKGS *F LQDILENEQFQLDWMFRLSLMHDIVRGMQFLHSSDIRSHGNLKSSNCVVDSRFVLKITDFGLHTLRRTRFDLESDGGNCN *F SHAYWSKLLWTAPELLRVEHNRPPEGTQKGDVYAFGIIVHEITTRQGPFYLGRCAYEKSPQEIIELVKGYNPHRMQKPFR *F PELEPNGDTKADINGIIRRCWAEDPAERPDFNTLKSMIRRFNKDNETGNIVDNLLKRMELYANNLEELVEERTQDYHEEK *F KKCEKLLYQLLPQSVAAQLISGQPVVAETFDQVTIYFSDIVGFTAISAESTPMQVVQFLNDLYTCFDSIVENFDVYKVET *F IGDAYMVVSGLPIRNGNQHAREIARLALALLEAVHNFRIHHRPEDRLKLRIGLHTGACVAGVVGLKMPRYCLFGDTVNTA *F SRMESNGEALKIHISETTKEALDEFGTFVTTRRGFVPMKGKGEMLTYWLEGEVPRPNSLISPSKLMLTRRSSLKQPQRSQ *F SHNLHKQYSELVVASPPPQLPPPAIALPPPPSPSKDSPNLRVKRKISSSSPKLNGGGFDYHKTENHYLDTAAAAQRNCDI *F YSSRSLRDMEETSDSWELAHFRLPLSGALKSHHHLNNNSHGYVHSNSSSNLSGILQPPPLGVQRTRLKPSPTISTLLTSA *F RSEANASPGPETGSMGSGQLRIKFAEGDETPLLPTPPPLAAPPPIPQMVAASPQRSGSGQNIPADSSHYGFLVKSYKQQC *F PPVAGSAVTQPLLAKINS *F >Gyc76C.AA *F MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGDLKTRQGLAISGALTMALDEVNKDPNLLPNVYLDLRWNDTKGDT *F VLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNK *F FSILYEDVWSPVADLLKDQATKRNMTINHKQSFIDNRVKCCEQMLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFM *F SSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRRVDQITFMSNCHSTENFNQMARSLLVVASTPPTKDYIQFTKQVQKY *F SSKPPFNLEIPRLFVESNFSKFISIYAAYLYDSVKLYAWAVDKMLREETRVLTDDVIFEVASNGTRVIDTIIKNRTYMSI *F TGSKIKIDQYGDSEGNFSVLAYKPHKWNNSNNMPCNYHMVPVAYFHQGEEHPEYKLINGSIDWPSGGEKPADEPMCGFAN *F ELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGS *F RWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIE *F NEDIKLDDLFIASLIHDLIKGMIYIHNSQLVYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWR *F APELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQINFEPKEIVDYVKKLPLKGEDPFRPEVESIIEAESCPDYVLACI *F RDCWAEDPEERPEFSVIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKMKTEDLLHRMLPQSVAEK *F LTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDR *F HAGEIASMALELLHAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKC *F KLALDKLGGGYITEKRGLVNMKGKGDVVTWWLTGANENAIQKKLVDMMDMPPPLFSRPRKSPKLNPDSRQPSIQAMHFCG *F TGSRRQSTVPRAMDGESTYSLQGSVRESPRMVSKRDRDRERPPINGLGAGHFVGGALLESAQASLSTLNHSETNETNCDM *F DGGSGGVSGSGSGLVRQPNALHKPLAMVRPHRIISAAQLPQLGDNDDDSADTLLRESRSLDPMPMQQLRKRHDRVKLPPS *F KLSKNNSRSLDTGVSLISGNPNGEVHSSQLDLDNEMTANPVDATDGYDDELGLLMRHDNGQLPALRYSGSFPNAQISIVP *F TGRSAGGGGGGREGGGSNCAKHLNNNCNGGVNVEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMDGPGGHGGNSVS *F YAADIDNRHPGDLDFFSGSSNQHHRSKAAGGSKLTNWMTNIFKGNGVRSGEARRVGILPSGVHGARTGFTDMAASAAARD *F RESIV *F >Gyc32E.AA *F MRDYASNVIRSHRCLCIFTVLQLTLMLSMLEVQIQIDQSPELQRPMTRVLLGAQLGELAWSPLFSGFAMGAMFYGYGLIH *F LPSKGPLGCFFSRLRTAGLSVWQQCSPVMLVPWTLKVAQGLSLFPCHLWEFLANEVCLETDNCLQHVILAFVLWIYLLIL *F AITSLVSVVRLNLQMLADRHIHSRDHELRAYVTLGGLPLAIEDVNKNPNLLPGKKLAFKPVDIGHKMSAYRVKPLRAMTQ *F MREAGVTAFIGPDESCTTEALLASAWNTPMLSFKCSDPIVSNKSTFHTFARTLAPASKVSKSVISLLNAFHWNKFSIVVS *F SKPIWGSDVARAIQELAEARNFTISHFKYISDYIPTTKTLSQIDKIIEETYATTRIYVFIGEHIAMVDFVRGLQNRRLLE *F SGDYIVVSVDDEIYDSNRRVNIMERNYLDPYIRKEKSKSLDKISFRSVIKISMTYPQNPHIRVPIYGLHLYDSVMIYVRA *F ITEVLRLGGDIYDGNLVMSHIFNRSYHSIQGFDVYIDSNGDAEGNYTVITLQNDVGSGASIGSLAKMSMQPVGFFAYDKN *F SVIPEFRYIKNDRPIQWLNGRPPLAEPLCGFHGELCPRKKLDWRYLVSGPLCALVVVVAIALLIKHYRYEQTLAGLLWKV *F DMKDVTVINLGEYNNPTNKNIFQICRQSILVVGEPNKRSFTNIALFRGNIVAMKKIHKKSVDITRSIRKELKLMREVRHE *F NIINFIGASTDHGSVIIFTTYCARGSLEDVLANEDLHLDHMFISSLVSDILKGMIYLHDSEIISHGNLRSSNCLIDSRWV *F CQISDFGLHELKAGQEEPNKSELELKRALCMAPELLRDAYRPGRGSQKGDVYSFGILLYEMIGRKGPWGDTAYSKEEIIQ *F FVKCPEMLQHGVFRPALTHTHLDIPDYIRKCLCQCWDEDPEVRPDIRLVRMHLKELQAGLKPNIFDNMLSIMEKYAYNLE *F GLVQERTNLLYEEKKKTDMLLYQMLPRPVAELLKRGDPVEAECFDCVTILFSDIVGFTELCTTSTPFEVVEMLNDWYTCC *F DSIISNYDVYKVETIGDAYMVVSGLPLQNGSRHAGEIASLALHLLETVGNLKIRHKPTETVQLRIGVHSGPCAAGVVGQK *F MPRYCLFGDTVNTASRMESTGDSMRIHISEATYQLLQVIGSYVCIERGLTSIKGKGDMRTYWLTKRQQPELTPDLISTVD *F TLDTYCSGPRESMEVSVHQYCSPASNNYRLGSCNCDTKCLYSRRSDDNVTNSHGTSEFPKVSEPAQVNCNQLCVCRLNSS *F QMFNNRGPRSAPSITFRL *F >CG9783.AA *F MLCCWLQQLQEFGGPEVDKPDLLKGSIGSLKNLGFVIPGAAAPGSAGAITTTANGKSGSSATGSLARHNPAHLDMRARYN *F GDLVQLKEVNINGSAELRTKAMDLLVMAHGLRHENINPLIGWLSDPNRTAMVFDYCSRGSLQDVLIMDEIKLDWSFRLSL *F LTDLVRGMRYLHTSPLRVHGALTSRNCVVDARWVLKITDYGLNSFYESQGLPPRTRSAKELLWTAPELLRNMKLHQHHHQ *F HGRIQLGTQLGDVYSFGIIMQEVVVRGEPYCMLSLSPEEIIVKIKKPPPLIRPSVSKGAAPPEAINIMRQCWAEQPDMRP *F DFNSVYERFKMLNHGRKVNFVDTMFQMLEKYSNNLEELIRERTEQLDIERKKTEQLLNRMLPSSVAEKLKMGLAVDPEEF *F SDVTIYFSDIVGFTTIAAHCSPVQVVDLLNDLYTIFDATINAYNVYKVETIGDAYMVVSGLPVKIPDHAEQIATMALDLL *F HQSGRFNVKHLPGVPLQLRIGLHTGPCCAGVVGLTMPRYCLFGDTVNTASRMESTGSSWRIHMSQETRDRLDARGGYAIE *F PRGLIDIKGKGMMNTFWLLGKKGFDKPLPAPPPIGLDESLIRNSITLKAQANKSRTSTNPSSSQSSSLAGESVEVKVEIT *F PPTNADLASGTNLPNSYSLDSNSTNTISPNATLCPEFPGKTTPSSTSPQSRKLSELTPENLLNPNSFNRLPSSTGGSSSR *F LYKKIEEMMDLSSPYNHYKCLSPSESNLTQFYDGKYLYGSVAGGAGGGQAGGGACASIALSGSGCGGGASRFDSKPGSSR *F LLRRQFSLDRDDQQAKGEQQHHQHSLQANTYSGLVGGCGGGVKSSMLDIPLLHDTTRSPKGTLTRSHKQNSASITQDLEK *F IEEIPLSPASSQHHSSLDSNLNRSPPSTLEAQSPPLPPMSPPQLSAPTSPAPSRTLNGIYAHNSNSTSSHGAANNNNGTH *F PGPELTLNAEQLLSR *F >CG10738.AA *F MFAHPCPAPAGNYHSGLLHPLLLLLFLLAFSNFRPTHGEVFTLGYLTASQRRPGNLDYNRPGLTISGAISLAVEEVNAGR *F LRDRGHSLQFVVAETYGEEVVSIRQTAALWTQQVAAYIGPQETCVHEGRMAAAFNLPMISYYCTHRDPSNKADFPTFART *F RPPDTQISKSVVALLLAFNWTQVSFLYLDDASGQYQPVAETILSTLTDAGVSIRDIRTWNTIYHHGFMDNPFEALVEQTY *F ANTRIYLILGHYYEHVGLMVSLQRRGILSKGDYFVVGIDIEQYDPAKPEKYLRGLLLEDVEPLAVQAFQSYLGIVPTASV *F SFATFANEVNKYMERPPFNFPNPLGPFGGVKQISAEAAYLYDAVHLYAKALMEVLDSGGRPRNGSAIVAAIKGSRYRSAM *F GYHVYIDENGDAAGNYTVLARGSVRNGRNQTVLGLRPVGTFIHRNSSLSSISKALPNLKLFSPIDWVGGTRPAAAPRCGF *F GGEKCVNYTGEISAAIAGGALLLLSLVSLVLYRNWRYEQELDSLLWKIDFREVQIHENEREQQSQKQTRSTHPLIRTSQV *F SLSSNPDADFRYTTIFTPIGLYKGQLYAIKKVRKKSVDITREMKKELKLLRDARHDNICAFIGACTDPPNICIISEYCTR *F GSLKDILENEDVKLDNMFIASMVADIIRGVIYLHDSPIRFHGALCTSNCLVDSRWVVKLTDFGLFAFKQGIEDSSTDMQH *F MSAKCLKLLYRAPELLRQGPSSLVMGTQRGDAYSFGILLYEMHVRRGPFGETGLTPMQCLQKVLQPQDYLNPYRPSLQPL *F ETAFDCVSECLRECWAERPEDRPDFKTIRTKLRPLRKGMRPNIFDNMMAMMEKYANNLEALVDDRTDQLQEEKKKTDALL *F HEMLPRCVADQLKKGHKVDPEHYEQVSIYFSDIVGFTAMSAECTPLQVVDFLNDLYTCFDSIIGHYDVYKVETIGDAYMV *F VSGLPLRNGDLHAAEIATMSLHLLSAVSEFKIRHRPTNRLLLRIGIHSGPVCAGVVGLKMPRYCLFGDTVNTASRMESSG *F VPLKIHCSWQCRQLLDRLGGYHFAERGVISMKGKGDQRTYWLLGEDEEARTRRTYERSQRRGSRALNKFIQGTIKQAQEQ *F ANEYGIRSSLKQKNLPRNSLTRSSSLESPKKLRFAAGSLLEHHRYHRFDNIY *F >CG3008.AA *F MSSPWVKTSEPVQQVNLADIMSEQYAHKLHDKELQRHTEKLKRPDEQIAPVWGSQASSPPAPNAQSIQSDAEDWEDYSAL *F LNDEGNVAEDIQLPEDSDAVIAQLLQSQFDHEYNEELRRIERQQNKQSKVTVTLNKFLRDGDAEFLHDTAEDDYEEDELE *F QQKHDWDRFEDNERQLDSIPRCGFKVNKEGEMITKHDPQLCAVRNAQRVMSFPPEFPTGDAAGFDMKLSNKVFNQLRAHS *F RRGRSDKHEKVATAEMGLDAGTRLLLYKLINNQILEQINGIISTGKEAVILHANSDANYTGSNEHGHQSGVLVPPQLLPR *F ECAIKIFKTTLNEFKQRDRYIKDDYRFKDRFSKQNHRVIINMWAEKEMHNLMRMQAIGLNVPDVVVLKKHVLVMRFIGDN *F HNAAPKLKDARLSDAELSCAYEEIVAAMHKLYNEAKLVHADMSEYNILWFEGKCWFIDVAQSVEPKHPSALEFLMRDCGN *F IVNFFERRGLPNIYTKEQLFEFITGLNSEVHTAAQLEQIHTRGASISQATAPNQEECPDELKPLEYPFELAWEKSQQDRA *F AQKALQQAQDNNDNKTDTDKDQETDDNENDSDDKEKINKTANH *F >CG11859.AA *F MGKLNVTVLRYLTKEDFRVLTAIEMGMKNHELVPGPLAAAIANLKSGGVHKLLKELCKHKLLAYERGKKYDGYRLTNTGY *F DYLALKSLTLRGSVSSFGNQIGIGKESNIYVVADEEGTPICLKLHRLGRTCFRNVKAKRDYHGRRHKASWLYLSRISATR *F EFAYMSALYDRGFPVPKPIDFNRHCVLMDLVKGWPMTQVHELLDAPQVYDDLMNLIVRLGNSGVIHGDFNEFNLMVTDAG *F KPILIDFPQMMSTSHENAEFFFERDVNCVREMFRRKFGYESEDYPKFSDLVREDDLDAEVHCTGYGFTKEMEQDLLEEYG *F MVDQADEEDEGEDLEEEEEPPTLVTAASVEIDECRRQVENEVIYSEAKPTQKSDDAVRRYIESCTQYLGNLSVGPEVPDQ *F TMPKKLDISLPAETPDVIPFEAETSATNTVEEDVKSISSNDLDTDEVPELVGLDPNSRMYRLKMVEQMLNDARSQRSYST *F TTSTIAPSVITDRIRRNMDIKEKREQRKKCVAKGEASAVHRHRKENKDVVKEYAGWDF *F >CG11660.AA *F MNNDSHKYSDAEEDEEDLRSSEWTNDFKNLTLKHEIFKDIKLTPKEDCDDLAQVIATANEEDEPEDEEPEEYDEDDYDDI *F GDDYDTYEEAYTGFNKLHVQPQLTNASGGGSGGGAGGASGGSQRVSSYQPNEKLLRRYSARINVEKYDPTTNMSAQAANR *F LVNFDRRDRTQVRDKHDRATAEQVMDPRTRMILFKLLNRGMIQEINGCISTGKEANVYHAVSKNGEEEFAIKIYKTSILV *F FKDRDKYVSGEFRFRHGYCKHNPRKMVRTWAEKEMRNYLRMRNAGVPVPEPILLRSHVLVMRFCGRDGWPAPKLKDVELS *F TSKARELYRDCVVIMWRIYNQCRLVHADLSEFNILLQDGQLVIIDVSQSVEHDHPHSFDFLRKDCTNISEFFRKRAVATM *F TVKELFDFITDQTITTENMEECLERISERIKDRDFDAISAQEKIDEAVWQNTYIPKRLDEVRHFERDVAKAKQGVQQNLI *F YGKITGLTSDLDVQQKPDVLQSSTAKEDDGSEAQTSGSEDEDNSQDNDNDEDAKKFKNSARPREESPESKKARKKAVKDA *F KAEQRKVKVKKHVKKRKEKMGSMKK *F >Ror.AA *F MNKYSAFIVCISLVLLFTKKDVGSHNVDSRIYGFQQSSGICHIYNGTICRDVLSNAHVFVSPNLTMNDLEERLKAAYGVI *F KESKDMNANCRMYALPSLCFSSMPICRTPERTNLLYFANVATNAKQLKNVSIRRKRTKSKDIKNISIFKKKSTIYEDVFS *F TDISSKYPPTRESENLKRICREECELLENELCQKEYAIAKRHPVIGMVGVEDCQKLPQHKDCLSLGITIEVDKTENCYWE *F DGSTYRGVANVSASGKPCLRWSWLMKEISDFPELIGQNYCRNPGSVENSPWCFVDSSRERIIELCDIPKCADKIWIAIVG *F TTAAIILIFIIIFAIILFKRRTIMHYGMRNIHNINTPSADKNIYGNSQLNNAQDAGRGNLGNLSDHVALNSKLIERNTLL *F RINHFTLQDVEFLEELGEGAFGKVYKGQLLQPNKTTITVAIKALKENASVKTQQDFKREIELISDLKHQNIVCILGVVLN *F KEPYCMLFEYMANGDLHEFLISNSPTEGKSLSQLEFLQIALQISEGMQYLSAHHYVHRDLAARNCLVNEGLVVKISDFGL *F SRDIYSSDYYRVQSKSLLPVRWMPSESILYGKFTTESDVWSFGVVLWEIYSYGMQPYYGFSNQEVINLIRSRQLLSAPEN *F CPTAVYSLMIECWHEQSVKRPTFTDISNRLKTWHEGHFKASNPEM *F >S6kII.AA *F MADVSDPSELFDLELHDLELQDDKARDSDDDRIELDDVDLEPELCINLHQDTEGQETIQLCEENVNPGKIKLGPKDFELK *F KVLGKGGYGKVFQVRKTAGRDANKYFAMKVLKKASIVTNQKDTAHTRAERNILEAVKHPFIVELVYAFQTDGKLYLILEY *F LSGGELFMHLEREGIFLEDTTCFYLSEIILALGHLHKLGIIYRDLKPENILLDAQGHVKLTDFGLCKEHIQEGIVTHTFC *F GTIEYMAPEILTRSGHGKAVDWWSLGALMFDMLTGVPPFTAENRKKTIETILKAKLNLPAYLTPEARDLVRRLMKRQEPQ *F RLGSGPEDAAAVQIHPFFKHVNWDDVLARRLEPPIKPLLRSEDDVSQFDTRFTRQIPVDSPDDTTLSESANLIFQGFTYV *F APSILEDMHRANRMPARSPRRTPRQLPDSSFRLQFPSANVGANAPLAMHGHSQRSGMFARATPPHHMQTFAPRPSPAQDE *F MMDVQGLPMV *F >JIL1.AA *F MSRLQKQNYEILSGTSTSRLKNHQHPRESESLAYEEPDQMVRNHLNGQLVANGNGKTRKNSNSETMTNGKKSKLNTEGSG *F SGSGKTLNYNNNNNNNNSISATNGQYTNSSSKTTSASARDYTYRETISPPTPPSPPTTNVADIVCISDAESEDGRDPERE *F YYDQDMEEDEPNGIEIDESSSSLSKAKSNNAAAAAAAAAAAAAAAASKASSSTTPSYAMPTSNSTPLDLDNEAHQRDLEA *F VTDLKYYVKLYSDEAVSLNDFKIIRVLGTGAYGRVFLVRKLTRHDAGKLYAMKVLNKITVVQKRKTAEHTKTERVVLEAI *F QRNPFLVSLHYAFQSSSKLYLVLDFANGGELFTHLYHSENFEESRVRVYIAEVVLALEQLHQLGIIYRDIKLENILLDGE *F GHIVLSDFGLSKILTAENEYRAHSFCGTLEYMAPEIIRTGPPGHDSAVDWWSVGVLTFELLTGASPFATSDGQVQQSEIS *F RRIQKEQPMIPSSFSANARDFVLKMLEKNPKRRLGGNHRDASEIKEHPFFNGINWQELRTKRRKAPYKPTLTAEDDVQNF *F SNEFTDQVPEDPECDAPPSRIRLFRGYTYVAPEHLEQMRRDNHCEIQYFNTGLQNIPCRPDDLELGTRTSNGAYGTCHFV *F VDSSTDLVFLAKIIPLSKFRPSEVDALISCALDTTNHKNIVSYHGTFREKCETWIVMEYLSGPELTASIRMDEDSCREIF *F LQLVMAVRHIHSKHFIHGDLKPENIMFENREDRTVKLIDFGSACYNNRFKSWKDKPRYTLDYAPPEMLADANLVTYSPAV *F DIYGLGATLYTMLVGHRPYRQNEDDVDHSAAAHHELRKRMRRGTFNQRSMRWESASPAFRHLVSWCLQRDPADRPTLSDI *F LDSEWLQYGSNDPDVDIILPQQMVVDLSEDTMEQPTGGMFDDQQQLEFMHDKSAEDEGITLVSEPMDTTVATHESRRNAA *F AFSSVVAPTTDDEIVHERFDPAFEVQADFYGFDENAPPLPLPEEYYSELPLPEEDRQYIPPPPALIPVEPETTFRRPRTR *F QQRRTESQLVQPVSVATYEDSKASLRVLMQQLPPPGDNVVARIPKRTHRVVRTLPPTFGTTKREENFYGFSKTAISWRKT *F RASWRHFCLLINGVQQVLKVRFKKARRVYCLPHIKEEKLDHAYEKPLTFPRPKAQLKRTKREPKVPRPPTRVQPERARAM *F RQLYQFQ *F >S6k.AA *F MPLADSQKDLRQQPQQQQQQQHVSSTSSSNNAEQQCSSSGLGLQLRQRMQITSSGCSSLAVTPMEHTPTEDDESGGGNSG *F VTSSVTTVTSSQRRQQLQQVQQQSALQAALEQHHISPTADLDSARKRPTHRTLCPPPELMELSDSESQGGVETGGRREGA *F TGRSAPDLEDTEPLYETENEFELKEVIKEGHDKADPSQFELLRVLGEGSFGKVFLVRKIIGKDAGTLYAMKVLKKATLKV *F KDRVRSTNERKILADVGHAFIVRLHYAFQTPGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALAMNHLHTLGI *F IYRDLKPENILLDEHGHIALTDFGLSKQPLDGSKTYSFCGTVEYMAPEIVNRKGHDFAADWWSFGVLMYEMLTGNLPFHG *F QTRQETMNQILRSKLGMPENLSPEAQSLLRALFKRNPQNRLGAGAQGILDIKAHCFFATIDWVRLERKQVRPPFIPAVSR *F DDAFYFDVEYTSKSPRDSPGGPISASAHEIFRGFSFVAPVLLEGQCAGSNMCSTSASPLHSIAPIPAAPVGAPRTLPGVL *F PGNFHAEYNLLQELGRGTFSVCRLCEHRASKKHYAVKVIEKAAVAAASTSTSADCWEEVEIMLRYGNHPNIVTLYSVYED *F AGSAYLVMELLKGGELLDRILAVGQMCESEASAVLRTIASAVAYLHEHGVVHRDLKPSNMIYASMRQTPETLKLCDLGFA *F KQLRADNGLLMTPCYTANFVAPEVLKRQGYDLACDIWSLGVLLYIMLSGRTPFASTPNDSPDVILKRIGSGQIDFTSSRW *F ALISVPAKELLRQMLHIVPENRPTAARILEHDWLREQFAGGVQLTEYAVAPGSQLSLGAQQQQQNHISMALRGAVDATFR *F AIAIPQAANVGPVELSMLAKRRAKDRANLHS *F >Pk17E.AA *F MGNSQPRNASRSRRTSQWPQGAAELGASASDHHYHQEELYQEQQQQQQQPQQEQPSSRRIQFQAHTNQPTQSHPPGDEEQ *F PVQSQQQQLSTWSLGSLSGGRLNWSFSGARNSFRHRNKATRKSLTSLHGSRRGKTQWHRPLTNSIFNSHFKETSKNDLYR *F IDHLVAKGAFGVVFKVSSKSDISQCYALKVLKKSKLIEDNSVRQIKDEADIQKVCGHHPFIVKQIDLWQNRHNLHILSEY *F VPNGELFSKITHFSIDLVRLYIGEIALALDFLHNAGIIYRDAKPENILLTEQFHIKLTDFGLSKWLKLGANTRTMCGTFK *F YMAPEILCGEPYGHAVDWWALGVIACQMLTQKSPNIKRHLLRRRESVEPEDGLSNAPSIAQINGCLQDSDGDSEDFLPEE *F VQHLTHEGRDVLRKLLTIEPRQRIRSVMALQRIAIYKDYNLSSKQLLSLSPREIIARDGIRIYEDRHFDQLTNQCAIDAF *F LDF *F >CG7156.AA *F MTLNLGGWIHSFTVTDTQEHKGGYTIYKITSIVFPRSVPQALTCLVVWKRFHDVKRLHRELSRRHKSLQLPGKLPVPTDS *F TYFKRFDAAVIQRRKEYILQLLDFAAQHPALYKCSTFTQFFQETPSPNGSPLKKLYVERSLRLQTEDNGCGSGGSETGTC *F AGAIADICDKLDLPYDPHDAGGITPLDPRCDKEQTNNESQAKETEIELETKQEPKTDVALTKRDYLRLLTPMASIESDDS *F DYIYEAALEFSHAVQAEVNLEYAEAHERYKHGVDLLLNGAKQDSSEERRFIAKAKIAKYLARAEEIHANFLANDCPTKKL *F QFQLSLDTTDGGSVSHLECPWNQLARYKVLQILQDRVMQVQCVTKSQQPAAIMKGVEKPASHSLTQSIFLPQQVPYMVQL *F LAYFQSEQKIFLLLRQAEGGMLYDYLQSYTPTSSKSVNYGELFPSDQEPEEKEAEEPPLTSDSDVSDLVRSSQQLLKSVT *F NTLSNLQAGVVTPKRNKKVDQNRIRSKPIPEQCLRQWACELAIAIHSLHGKGVILRDLHMGNILLGAKGQLLLTYFYQNE *F GLTSDDNYVHKALNLEAVANHFVAPERPLTFRSDWWSYGVVLYQLLLGVPYKVTHPGQLDLYGCVQYPSESLEISDHAKD *F LLEQLLQLSPELRLDYDQLQAHPFFKGIDWQAVLEQGTESM *F >drl.AA *F MAPNLLTIGLLLTLIASGQAHLNIFLNLHEVLRLIGVSAELYYVREGAINDYALNFAVPVPANISDVTFTWQSLVDHPLP *F YSINIATSDTEVLPRPILNISRIGDVPVEPQTWGIALKCSGTRNAEVTVTINVEVILDRATNNNTNLIFKRKKICLREEQ *F DSAHEEYDDDDLDLLQTARKGHGGDIHYVDRNDEHVVANGHQAPEKQRPVVTESPVGRGNSGGSKRDFDPMLRENLVPPA *F SGLVTLIVGGILALVLVSTLILIAYCAKGPSKRHPSNGVHLIKTSSFQRLPTISSTAHNSIYVCPSTITPTYATLTRPFR *F EYEHEPEEFNRRLQELTVQKCRVRLSCLVQEGNFGRIYRGTYNDCQEVLVKTVAQHASQLQVNLLLQESMMLYEASHPNV *F LSVLGISIEDYATPFVLYAATGSVRNLKSFLQDPSYARSVTTIQTVLMGSQLAMAMEHLHNHGVIHKDIAARNCVIDDQL *F RVKLTDSALSRDLFPGDYNSLGDGEYRPIKWLSLEALQKSHYNEGSDVWSFGVLMWEMCTLGKLPYAEIDPYEMEHYLKD *F GYRLAQPFNCPDELFTIMAYCWASMPAERPSFSQLQICLSEFHTQITRYV *F >dnt.AA *F MSRKMWVLSLLALAALQLHSGSEVAAHLNVFLNPVEVMRLLGVSAEVYYVREGHINNYALNFIVPVPANVKDISFTWQSL *F AGRGLPYSINVVSSDQEVLPRPAINVSHSGEIPTTIQTWSIALKCSGLKAAEVDVTVSLEVVLNRSLNNVTHLVFRRKKI *F CLMNDSAEDLSEDVDDPQLLETVMLPPTGLITLVVGVSVAMGSVCLLLMIAYCVKGAANKRQHHQHGGQPMRTSSFQRLN *F THPPCQSSMGSAAYMTPSIIAPIHGSSLPRKVPVSVEQQHPEELHRRISELTVERCRVRLSSLLQEGTFGRVYRGTYNDT *F QDVLVKTVAQHASQMQVLLLLQEGMLLYGASHPGILSVLGVSIEDHTTPFVLYPALNNTRNLKQFLLDPACARTVTTIQI *F VMMASQLSMALDHLHSHGVVHKDIATRNCVIDDQLRVKLSDSSLSRDLFPSDYNCLGDSENRPVKWMSLEALQHKQFSEA *F SDSWAFGVLMWELCTSAKQPYAEVDPFEMEHYLKDGYRLAQPFNCPDELFTIMAYCWALLPAERPTFAQLQSCLSEFYSQ *F ITRYV *F >Drl-2.AA *F MHKVCSAERISGSNGVGVPSKLRGNFRFTSAVHPHPQLSLRPVGSFIPAGLEADLFYVHEGAINTYAMHFTVPVPADVHE *F LEFSWQSLIAYPLPYAISIEYNNDQEALGTPTLSIPHKGLVPQEIESFLVYLPCTGNASLQMPVNVNMVVRAPPRFNDTR *F LHFKRNKICAKVDQSQPASQGSPRPLSHPTRRDSFDDGVRYCCVLIEFSLWAKSSSKRGSNSSSGSTTNTIKDKQQDLST *F PKSIRSNGNGNCTSGSGSLSLFGGIPTGSTITMASHGEKGNQRLRRITSVQPGALSYEELVKEGTFGRIYAGKLGESCEA *F LVKTVIDGASLTQVACLLQDASLLIGVSHQHILAPLLANTELPGPPEIAYPHPSKGNLKMYLQKSRESSTALSTRQLVEF *F GLHITKGLAYLHSLGIVHKDIATRNCYLDEESYVKICDSALSRDLFPDDYDCLGDNENRPLKWLSLESLQKRVYATQGDV *F WALGVTYWELVTLAQMPHEEVDIFELTNYLAAGFRLEQPVNCPDEFFTVMNCCWHCEAKQRPTPSQLLSYLQDFHADLGM *F YI *F >BcDNA:LD22679.AA *F MLSVFEGKVFVKAHVWSNGVGPIERAIKNLMVYRHPYILKYIATWEKSGRKYLATERVRPLDEVLAQQTDIEVCLGLRTI *F LCALIFLVEKALARHLNINTQSIYVTESGSWRLAGFEYVWRATDVNKQLLDLAHSFIDLTIHGENFEQFFFSILCEKVLS *F RKGTDSCITDSTPHVHEFREYCSTHLKHQNTKLRPRLSAILLHPYFNHEFVLIHSFLFELPLKSVHERHKFFRSLIDRLR *F YFDEEVVASQLACDLLSRMVLLDPAAQEFVTPHILRTKVTDKAPASLFSPQIYVQYLMPHILKMFRLRDAQIRLILLDYF *F MDYVRLLSDEQLESEILPHLQLGMNDTNDVLVGKTLRCMADLVSILGANKVLGGDRARCFSDGRPHAAVSRDSNNPYPEP *F RSISPLMNTRSFDVEDFMVSGSPLPQESNASPLSIRLSPDGGEDEKLRLNSNEKSISIKHNIDPEKDSYISGTEHERVAN *F IDEEGTWLDWDNTDHLQQGYQVDHAVTDSETNSNSFAREIQTGKSLSPSYRTGKCNLSVGVNETISQTEQKVIDDLSELD *F IKVQTVIQRSEFNEFDFFKDMEPIIEIRTSTCETPEQISSRFAAAASAVNCNELCADQGWGHDEQDDKDDIVWGVTNVST *F L *F >CG1973.AA *F MWSFFSRDSSKDFPYDIGEPVGGFDQYSIWTLHKAKRKTTLEEVSVFVYDIRSGSDTKCELAKAALKRLKTLRHPSILQY *F LDSLETDKMLYVATEAVDPLGTYFSKLGSDNVQKGLYLAWGIFQITRALSFLNNDGNLRHNNVSAWSVFVNASGEWKLGS *F LEYVSAADGNPMPPAKIPVTLEVYDSPEKNDPSKLKAATKCSVDMWGLGCLVWEAFNGVLKQRSNLKDIEHIPKSLQSLY *F CELVGASPSNRPNPADIITRCRKPGGFFKNDLVDTLLFLEEIQIKDKAEKNRFFSGLTTHLDNFPDNVCRHKILPQLITA *F YEYGDAGSAVLAPMFKLGKLLDEVEYQKRIVPCVVKLFASTDRVTRSRLLQQLDLFIAHLQPQVVNDQIFPQVAHGFLDT *F NATIREQTVKSIIHLAPKLNYNNLNVEVLRHFARLQARDDQGGIRTNTTVCLGKIAPHLHPQVRQRVLVSAFIRAMRDPF *F PPARVAGVLALAATQQYFLLSEVANRVLPSLCSLTVDPEKTVRDPAFKTIRGFLGKLEKVSEDPSLRETMEADVHTATPS *F IGNAAATWAGWAVTAVTAKFYRSQSDSSRPRPPLTGRNLSKPASLEQPSSSSLSTTTSSVTSMTSLEHESNDTSASASDY *F GNNDWDNENWGEMDTSQDPSSPLAASSNNGALMAANALSEVRDGWDNEEWGSLEEDPCEEEEQAEQEQQQQQLARQSISS *F TSSSQPNQRPLLPHQQQYPHQQQLQQHELNDLIEPLAKLNSHVTSPSKQSMLRPKELPNLSSSNTSPTSATANCNNISPP *F SSHLNNSTHNANWNSDSWADGEFEPLDESGFGNAKLDEARRKREEKKLQRQRELEARRAQRASGPMKLGAKKL *F >CG1951.AA *F MDMINKFYSSAVQTVSTLSGVLPGNNVTREYEVLEQVCTAGVGLMWKVYNGHKKSTKQEVSVFVFEKKSLERWSKDDRET *F MLETLRRGVQQLTKIRHPHVLTVQHPLEESRDSLAFATEPVFASLANVVGDNVRSEKKLYDVEIRHGLLQLFDGLQFLHQ *F DAKIVHRNISAETIVINKNRSWKLFGFDFCIANQPATDGTPHWPFREYTTSLHVLAQPSLEYTAPELALNSVNTPDSDLF *F SLGVLIFTIYAGKPLKMFGSDYSSFRRYANDLNQRKYPPMNAVPSELTESLKALLHPSANLRPKLHELKQIAYFQDVGVK *F TLSYLDSLYQWDNLQKSKFYKGLPQIIPTLPHRVNLHSILPYLVKEFVNSPMIPFVLPNVLLIAEMSSQREYCDHILPHL *F KPIFKLTDPIQILLIFMQKMDLLLKLTPAEEVKQSVLPLLYRSLECDMPQIQDLCLAVLPTFSTLIDYNAMKNSVLPRIK *F KLCLQSSNVSVKVNCLISIGKLLENLDKWLVLDEILPFLQQIQSREPAIIMGIIGIYKIAMTNTKLGITKDVMAHKCIPY *F LVPLSVENGLTIAQFNTIISLIKEMMGRVEQEQREKLQQLSTIQRDNKPKDASEILANELENSTLSPYGSINGNKINDDM *F FSGFTVGQPKAAIGAAIAPAKAREQLKISHNTISAPPAARPDILSSMMQSNLTGLGTITVAAAPPPTTTQMPSNNGWHSA *F NPLVMNHQLASPQASNNNSFSLDDLDPFAGGRPSVGAPKANNTNGANMYTVQQPTVNYIYPTGYSLNSIQQHQQQQLLGK *F PSQAPTLQPQTQPLLPTDNQNLNALSQQDILNFLN *F >CG14163.AA *F MPAGTMFAKFKSTNVAASTATQAVADGNPITQYFEIGKPVACAGPELVWRIHDAYRKSDNKECSVFIFEKKIAEKLHKPR *F RKETITELLKSSVKTLERFRHPRILQIYHTVEESADTLAFASEPIFASLSNVLAFHESKTYEATNVAPAAGGAAASQAQA *F AANTLPQRPAHAKEYNFLDMELKYGFLQLTEALAYLHYSGHVIHRNVCPSSILITKRGIWKLAGMEYVERMNENDLNSSI *F PCPPWSNRVSKMAQPNLDFMAPETQSTSKCSLLSDMFSLGMVICAVFNNGRPLIQAGNSTSNYAKQMETLDDLVHKLMPR *F LPIALQEATSRMASRDATARPTAQLLQLIKYFIDTPINALKFLDVVNMKDTQQKSQFYKTTLIEAMPMIPRKLWWQNIWP *F MLKSEINNNEVLAAVLQPVMLFVQEATLTEYDTLMSATMKVIYNTPKSIQASVTILENLHLIIEKTKPEDVTTDIMPMLF *F YSFDGSTIQVQSAAVVAVANVFDSIDELSIRRMVLPKVKQVFEKNITDPKIVQNVLMCIERVMDRMERAQVMDEVLPLLA *F NIRIPDPDIIMRTVRIYHKLFVDKTYGLTVETMATNVLPLLIPHTVNPSLNFEQYCYLLEVLQQMLEAIDRQQRNKLKLD *F NLSMASPERHRTLRHQFSTDNMNAPPFNIPNLRIDQRKTSSAEDMARKNSGGE *F >sev.AA *F MTTTHINQQAPGTSSSSSNSQNASPSKIVVRQQSSSFDLRQQLARLGRQLASGQDGHGGISTILIINLLLLILLSICCDV *F CRSHNYTVHQSPEPVSKDQMRLLRPKLDSDVVEKVAIWHKHAAAAPPSIVEGIAISSRPQSTMAHHPDDRDRDRDPSEEQ *F HGVDERMVLERVTRDCVQRCIVEEDLFLDEFGIQCEKADNGEKCYKTRCTKGCAQWYRALKELESCQEACLSLQFYPYDM *F PCIGACEMAQRDYWHLQRLAISHLVERTQPQLERAPRADGQSTPLTIRWAMHFPEHYLASRPFNIQYQFVDHHGEELDLE *F QEDQDASGETGSSAWFNLADYDCDEYYVCEILEALIPYTQYRFRFELPFGENRDEVLYSPATPAYQTPPEGAPISAPVIE *F HLMGLDDSHLAVHWHPGRFTNGPIEGYRLRLSSSEGNATSEQLVPAGRGSYIFSQLQAGTNYTLALSMINKQGEGPVAKG *F FVQTHSARNEKPAKDLTESVLLVGRRAVMWQSLEPAGENSMIYQSQEELADIAWSKREQQLWLLNVHGELRSLKFESGQM *F VSPAQQLKLDLGNISSGRWVPRRLSFDWLHHRLYFAMESPERNQSSFQIISTDLLGESTQKVGESFDLPVEQLEVDALNG *F WIFWRNEESLWRQDLHGRMIHRLLRIRQPGWFLVQPQHFIIHLMLPQEGKFLEISYDGGFKHPLPLPPPSNGAGNGPASS *F HWQSFALLGRSLLLPDSGQLILVEQQGQAASPSASWPLKNLPDCWAVILLVPESQPLTSAGGKPHSLKALLGAQAAKISW *F KEPERNPYQSADAARSWSYELEVLDVASQSAFSIRNIRGPIFGLQRLQPDNLYQLRVRAINVDGEPGEWTEPLAARTWPL *F GPHRLRWASRQGSVIHTNELGEGLEVQQEQLERLPGPMTMVNESVGYYVTGDGLLHCINLVHSQWGCPISEPLQHVGSVT *F YDWRGGRVYWTDLARNCVVRMDPWSGSRELLPVFEANFLALDPRQGHLYYATSSQLSRHGSTPDEAVTYYRVNGLEGSIA *F SFVLDTQQDQLFWLVKGSGALRLYRAPLTAGGDSLQMIQQIKGVFQAVPDSLQLLRPLGALLWLERSGRRARLVRLAAPL *F DVMELPTPDQASPASALQLLDPQPLPPRDEGVIPMTVLPDSVRLDDGHWDDFHVRWQPSTSGGNHSVSYRLLLEFGQRLQ *F TLDLSTPFARLTQLPQAQLQLKISITPRTAWRSGDTTRVQLTTPPVAPSQPRRLRVFVERLATALQEANVSAVLRWDAPE *F QGQEAPMQALEYHISCWVGSELHEELRLNQSALEARVEHLQPDQTYHFQVEARVAATGAAAGAASHALHVAPEVQAVPRV *F LYANAEFIGELDLDTRNRRRLVHTASPVEHLVGIEGEQRLLWVNEHVELLTHVPGSAPAKLARMRAEVLALAVDWIQRIV *F YWAELDATAPQAAIIYRLDLCNFEGKILQGERVWSTPRGRLLKDLVALPQAQSLIWLEYEQGSPRNGSLRGRNLTDGSEL *F EWATVQPLIRLHAGSLEPGSETLNLVDNQGKLCVYDVARQLCTASALRAQLNLLGEDSIAGQLAQDSGYLYAVKNWSIRA *F YGRRRQQLEYTVELEPEEVRLLQAHNYQAYPPKNCLLLPSSGGSLLKATDCEEQRCLLHLPMITASEDCPLPIPGVRYQL *F NLTLAKEPGSEEHDHGMEPLGQWLLGAGESLNLTDLLPFTRYRVSGILSSFYQKKLALPTLVLAPLELLTASATPSPPRN *F FSVRVLSPRELEVSWLPPEQLRSESVYYTLHWQQELDGENVQDRREWEAHERRLETAGTHRLTGIKPGSGYSLWVQAHAT *F PTKSNSSERLHVRSFAELPELQLLELGPYSLSLTWAGTPDPLGSLQLECRSSAEQLRRNVAGNHTKMVVEPLQPRTRYQC *F RLLLGYAATPGAPLYHGTAEVYETLGDAPSQPGKPQLEHIAEEVFRVTWTAARGNGAPIALYNLEALQARSDIRRRRRRR *F RRNSGGSLEQLPWAEEPVVVEDQWLDFCNTTELSCIVKSLHSSRLLLFRVRARSLEHGWGPYSEESERVAEPFVSPEKRG *F SLVLAIIAPAAIVSSCVLALVLVRKVQKRRLRAKKLLQQSRPSIWSNLSTLQTQQQLMAVRNRAFSTTLSDADIALLPQI *F NWSQLKLLRFLGSGAFGEVYEGQLKTEDSEEPQRVAIKSLRKGASEFAELLQEAQLMSNFKHENIVCLVGICFDTESISL *F IMEHMEAGDLLSYLRAARATSTQEPQPTAGLSLSELLAMCIDVANGCSYLEDMHFVHRDLACRNCLVTESTGSTDRRRTV *F KIGDFGLARDIYKSDYYRKEGEGLLPVRWMSPESLVDGLFTTQSDVWAFGVLCWEILTLGQQPYAARNNFEVLAHVKEGG *F RLQQPPMCTEKLYSLLLLCWRTDPWERPSFRRCYNTLHAISTDLRRTQMASATADTVVSCSRPEFKVRFDGQPLEEHREH *F NERPEDENLTLREVPLKDKQLYANEGVSRL *F >CG8726.AA *F MAVFASRYERKLPIDDTQALSCEITAVQEVAGHTEYLLRVWRGASNKNYWTVLRRYNDFDRLDKSLRVSGIELPLPRKRI *F FGNMRPEFIAERKQALQEYINAVLMNPILASSLPAKRFVDPESYSQSFHDHAVQNAMLCLRNDTTWSLGGSMGAIGWRLR *F KHYFKVTTKPPEKSSNKQLVKSGSQTHQSKHFAAGSSNGSGHSIDAGTLDPGSEVVAEWLEYGPDKFIDEKEIGGIMKSL *F MGLQHPHIEPVLLAAHTENGCLVIRKFHKHGTLKDVLCMAANPKNTFLSKYGNPKGRTALSMKQVATYGKQILEALIFLH *F SKGYAYGHLHSGNIVIVDDCVKLLDIENFLLGVPAFYRPFFMQHSKIHAIETIDVYCFGHVLFEMAMGYPLQESVVRQIT *F ECPEALKCLLESILSKEACKAGLPTLEQLLGHRFFLQYASTESAGAAANAEKPYFKLSLNAKELLKQAAIKSENRLRDEQ *F KSVKNQKRIVRVQELMSSEEEKRKSKQKAKLEHKQSKLKQQSSIQTNNGRLSLVAATAAAASSSTTVVGELCGTDSFNRS *F DSTPEEPTTLAGIKSPPLTPGPHLGSIYQQELPSTTGAPVERQSSTPNMLSEDAEGGDGDEPTRSALLESICKFNRGSLR *F KVRSND *F >Slob.AA *F MFKFNKAAQQQRIDNRNSAVTGHDPFVRPPVPEKKVRNIMKKLHKANGLKRSNSAIEFDVSALTAANRRQIYSSNRSASS *F EQDNSDLSEHSEKSPLVSARLDNLARLFFSKSMPAETGSRDTIDSVLTTRPNIKYQYSALDSGNGIVERSPRERAQREKA *F LNATQEWIQGANGRYEVIAHLDEIGSRHGKNWFLVTDASVRTDRLQTLLPLPPDCVAFEDLPPNECAREILMELLGSLHH *F PYIYPVLDLGFLRNSSYNYACLVTPFNSRGSLKDLIYKAQWNEPWARKYTRKPNGLPVSQVQRLGRQILEALLFLKERGF *F PLHGHLHSGNVILQNGAARLSGLENGLLGLSSRINAVMWSRSVTEIENVDIVCFGHLLYEMCTGQELTTPKPSMRVLEME *F LQHYPQIGQTRKQILEILGLIFEPPSGVCPSVEDLVLCDLFRSIDLRELRGPCFSTIKPSLSRSTLNLLQAVKKRQCASL *F GHSLSEANSPCTPPSTPHDRRTGVLPAPYEVFRMY *F >Src42A.AA *F MGNCLTTQKGEPDKPADRIKLDDPPTIGVGVGVPQIPMPSHAGQPPEQIRPVPQIPESETAGANAKIFVALYDYDARTDE *F DLSFRKGEHLEILNDTQGDWWLARSKKTRSEGYIPSNYVAKLKSIEAEPWYFRKIKRIEAEKKLLLPENEHGAFLIRDSE *F SRHNDYSLSVRDGDTVKHYRIRQLDEGGFFIARRTTFRTLQELVEHYSKDSDGLCVNLCKPCVQIEKPVTEGLSHRTRDQ *F WEIDRTSLKFVRKLGSGQFGDVWEGLWNNTTPVAIKTLKSGTMDPKDFLAEAQIMKKLRHTKLIQLYAVCTVEEPIYIIT *F ELMKHGSLLEYLQAIAGKGRSLKMQTLIDMAAQIAAGMAYLESQNYIHRDLAARNVLVGDGNIVKIADFGLARLIKEDEY *F EARVGARFPIKWTAPEAANYSKFSIKSDVWSFGILLTELVTYGRIPYPGMTNAEVLTQVEHGYRMPQPPNCEPRLYEIML *F ECWHKDPMRRPTFETLQWKLEDFYTSDQSDYKEAQAY *F >Src64B.AA *F MGNKCCSKRQDQELALAYPTGGYKKSDYTFGQTHINSSGGGNMGGVLGQKHNNGGSLDSRYTPDPNHRGPLKIGGKGGVD *F IIRPRTTPTGVPGVVLKRVVVALYDYKSRDESDLSFMKGDRMEVIDDTESDWWRVVNLTTRQEGLIPLNFVAEERSVNSE *F DWFFENVLRKEADKLLLAEENPRGTFLVRPSEHNPNGYSLSVKDWEDGRGYHVKHYRIKPLDNGGYYIATNQTFPSLQAL *F VMAYSKNALGLCHILSRPCPKPQPQMWDLGPELRDKYEIPRSEIQLLRKLGRGNFGEVFYGKWRNSIDVAVKTLREGTMS *F TAAFLQEAAIMKKFRHNRLVALYAVCSQEEPIYIVQEYMSKGSLLDFLREGDGRYLHFEDLIYIATQVASGMEYLESKQL *F IHRDLAARNVLIGENNVAKICDFGLARVIADDEYCPKQGSRFPVKWTAPEAIIYGKFSIKSDVWSYGILLMELFTYGQVP *F YPGMHSREVIENIERGFRMPKPTNHYFPDNIYQLLLQCWDAVPEKRPTFEFLNHYFESFSVTSEVPYREVQD *F >BEST:CK01209.AA *F MLLSLAGRLHLLSRHILGTLGQDPIFMGVLQGSLLMLFLFLMHLLRLWSCGYAVERPLNPTPDVAKELSCDFNFVSEQEK *F RAAQEAATEALLEVEEKKRRKRFTKKRNCVSAGPILIALRHQSKRPKHHRHAALPFEPVTPGEIILDTYLPPRELPLTIR *F LPQLAEPVKEEEVEYIELKSEPRGTLSTTDEIYPDSSDSSLYVSDEEQEDASQYCRGGYHPVVIGDIFDNRFRVVRKLGW *F GHFSTVWLCRDLKDEKYVALKVVKSAPHYIETAADEIRLLEAIRDADPMDVKRERIVRLMNHFTVRGVNGMHTCLVFEAL *F GCSLYKLIVKNNYQGLAIAQVRNIIRQVLEGLDYLHSKCSIIHTDIKPENILLVIDNAAAMNQQIDDEINSLRVKGVDFP *F DSYISSIEKQTKSRAKWPLIEPNGSTNTNTNTSNSTATNSNSSTPLAAVIMSTLDKEDTTTTTSSTLNSNTTSSLASKYS *F SLLGDSECNGGLGGSANINNRYRTEKKITAKSSGDCDEDAESDTLGEQSTLASTMDSPTDLDPEPELDSKPNTVPEPTTI *F AKSKINSNILSTCTSLTSTNEYCFTNTNTTTASTPSAPPSATPSSAPALASATPPSTCTPSFTQIAPSATAPATSTTCTT *F SAEFYDGDHKTTSTSTTTTSNAISSATTTTATTTTAIAKLNVHANAIPSQNQSQSSQNNTYTIQSLIDNSNVRVKIADLG *F NACYDYHHFTEDIQTRQYRSIEVLLGAPYNYTADIWSTACLAFELATGDYLFDPHAGESYSRDEDHLAHIVELLGSIPQS *F VIFRGKHGLKYFTSYGSLRNITKLKPWSLMNVLVEKYDWDPVEAKKFSDFLLPMLEYNPVIRASAAECLQHPWLEQEEFV *F *F >CG8565.AA *F MFKMKRTRVIRRKAAIIRASKRAFQEKIRKQMESCGVTQMGNASTQQSNVPMDVDADMGRKMSEDGMTDTSNTVKQPSCS *F KDQKQLSRKQKPSPSYNHQEQIEQRAKRPKICKKRCKQKRRMPSSLSDDGIAGRKPAKINKYSELSYGSESSMPFEMKQQ *F SNDNQNNNDNAEGSGFDRQESANEYVIGGYHPVAIGDVFVNRYHVFKKLGWGHFSTVWLCYDTQMDRYCAVKVSKSAQVY *F KETGIDEIMLFSQMSLHDQHKYRSHVVGFYDFFEITGPHGRHICLVLEVLGDNLLKVIERCFYKGMPISNIKQIAQQVLT *F GLKFLHEECGIIHTDLKPENVLLASNEVSVRTEIKTAIEVYLKANEGKLSPSSKMTKTAKRRMQAKSKKVISFFKNHRRM *F LRRQGIEDLLYLAERGLIEPITAAMAVSDNLPFIPFGFDGFMMMSDADCRTVQNSKLPEMEGMEYKCDRINLCLKSPELF *F LRYVLDIIKNLDEKELSTQDKRRRFTQASGRGQTKKNARSRLRLASNAAYTTGTGWRSNRKINLNDIYPIDPANNECDVR *F VKIADLGNACYFHHHFTDDIQTKEYRALEVILGAGYCETADIWSVACLLWELATGTYLFDTHSKRGKYNLDEVHIAKIVE *F TCGRIPWYLIRKGKHSRNFINSAGKLCNIETLKPLKLANILIRWYGWRTRQSTEFVNFLMPMLQTNPLSRISASKALESH *F YLCNIALPGIDIDSYYYYSVREIKDCTHTKSERNSDEDSSSCSSIDSSEFSSDEVCYEDDVNEEDIKKLY *F >SRPK.AA *F MNKADVNRRVLAIQAKKKRHKSRKRGKQNGTNPQEQSNSSQNQNPKPSSNPIPEPNRSSENLQAPDNANNSHLNAPSSAA *F FSKATATATASTSSGGSAGTPDQYQPPKKTQAKAKPNTQKEHQQQPPRSSSNESYESETFSDNEDQELMEDYCKGGYHPV *F NIGDLFHDRYHVIRKLGWGHFSTVWLCWDLQAMGYVAIKIVKSAPHFAETARDEIKILKTVRETDPSNPRRHKTVQMLDD *F FKITGVNGTHICMVFEVLGDNLLKLIRKSNYRGIPLANVKTITRQVLEGLDYLHTCCKIIHTDIKPENVLLCVDEPHVRS *F LATEATQLYCMNSKMYPSLVSRAPKEYREPPITGKMSKNRKKKLKKKAKKRMELFKQQRDYLEQADGQGAINPNEVQNGD *F AGISDADEYFDANSVEDNVHVAAGQPSRKQRDERKAPPEQSEEAQEQDPLTEGTDKAKKKKKKKNKNKSNQSKGQQPPQL *F ENSTSSAESSSALKSQQANGSNSTTNNKSNTNSSGTLKGQSNGKKMPLRPKQEKQGSNHQLNNNNSKPNSNSDSKISSGS *F VENTSSATNGPHSNSTLPTPPPPPQAKHKAKKDPALDECNVHVKIADLGNACWVDRHFTEDIQTRQYRSLEVIIGAGYNT *F SADIWSTACMVFELATGDYLFEPHSGESYTRDEDHLAHIIELLGPIPREILLNGTYAAKSFTRSCELRNISGLKPWGLMD *F VLLEKYEWSQKDAASFASFLTPMLEFDPNKRATAAECLQHPWLR *F >Pk92B.AA *F MDTAVGDHLEDRQCALEEIKQAVQSAGANFQRVQFERLDFGETNVLETFYNADVAIIDLSILTQQRPLSYHYGVRESFGM *F KENILTYNDIDSKQTLSLKLSCANYLFLSYKRNAETNSCHLTSQPNSGNNSKEPNAEGRVPTLQWRLKRKLQDVEIQSKA *F HMREKFLSDMRTARDTYATNGAKLQSILHEMRKRLDDPHVLSGEVVHSFMCSLRDVQDYDAMVRLVNDLKNIPNTRKYVE *F TGNMSFLYAFALNRRNRKGDREKALESSLKALEKKENHFPDMLCLCGRIYKDIFVESDYTDATSLAHAIKWYRQSFEVQP *F NEYAGINLATLLVIEGKEFTNTEELQHIGMTLNNLIGKKGSLSSLSEYWDVATFFEISVLANDYAKAIQAAECMFKLKPP *F NWYLKSTIGNISLIHRFRKKPEERQPPIEEQVFQFWMDFFLEATNTEEVKNSIRFPILILEPQKIYMPSYVTINMDADEK *F SIQIVNICLAHAKNACKKIHDFLFVASKIKSVSLYKRDDRCAYLYVHHNSDDFQIYFPSTERRQKFYDMILEMTADQVVF *F VNLSNDDANIEYEYDYDEQNRKMVLGKGTYGTVYAARDKQTQVRIAIKEVPEKNSQDVQPLHEEIKLHSQLRHRNIVRYL *F GSCSENGFFKIFMEQVPGGSLSDLLETKWGPLKDNESTMAFYSKQILEGLKYLHEQDIVHRDIKGDNVLVNTYSGVVKIS *F DFGTSKRLARINPMTETFTGTLQYMAPEVIDQGVRGYGPAADIWSFGCTNVEMATGKPPFIELGSAHAAMFKVGFYKKHP *F NIPEELSANAKNFILRCFAISVMDRPSASQLLEDPFLQDKPRKVRPALPINTEFGRSISVPADRFVHKTTPPLSYNTTCN *F TPTTPELDITHSSSVDIDELPSNQFMLERRNSHGFLLSPEIEPSTPSLRTSISETSETDGFYRLKKDSQRRTTLSKVLAL *F DESKICDIWLKKVDADQNSIAIRKSDLEDAVVCVLRYHCIKPHWMFALDNLVKRAVQAAVTIFSPELGANLADKDLSGND *F DESLRTSLMQKGSTESQEKQSLEKVLPTSNTREGVGLTPAESGGDALSSSMECARILRDIRDNQQKLQRQLLEQQRQSMR *F ALHNISQELAHIYMGGRKRLRRTINGFNKKCHRMSTSVGGTSGVRPNIAGNPRRQLNKHHFGLHEVDEQLEQWLTQQEID *F EFSKTLILNEGFTFDDFIYNMEKLDLMRLGLRVGIEVRLWKLIIQERVCTSSDCVDSPPSTYHKPIGGNSSFALIDNNNT *F LPSPTTTTPGTPGIKTKIKKSESEYDSCSE *F >Mekk1.AA *F MSNRRRVRTIDYLALQQSLRLQKTPAATTNAEEQVAREEENGNGHHSTVTAETPPTPPIPPIPPIRLRREQSVEEDVARV *F EYRVKQTPSRPVQMTRNRIGALEEDLPPEDELAAHYEAFGTTPPRTRLKIKNRDWERKQKVVNVTASADLPASVGGTPKK *F TRTARSRVLRRNTMDCALLNEMFVNDESKRTDKRLQSLLRDSEREMKNSLATTAVAAPRGNSFHETAHPLESLDQIMPLN *F SEAMAPIPLRIASKVVESCNRYRCVSSRPIGYRSSAPPLAFAAQLPAGMLRSDGRATPGLGKRKDFHETFANLIKLGSVD *F RQDAKLSQEEHTWQTELKDLIWLELQAWQADRTVEQQDKYLFEARQGVSDLLTHIINYKFQPRYRREPSLISLDSGTHSD *F SNSNASSPLPSKMCQGCMSLYCKDCMDHQKLALREVEGMLTRLEAAEALYPSSQAMGALHPIYKSQSFVGRIKSMCLWYN *F ITKQNKLKLSILGKILARLQDEKFSWPVCTSSYIATDSGSSSASGVENDDSAVNSMDSSKPPSMAGSASRKGVTPCHKVQ *F FMLNDATHVPGETSSSNESTSTEVSQWSSECSHSHMRKGSMHDINIFSVEPLGTCSSNGTGEQSTGLYRKFIENVLKSRG *F LAKSLAFLHKLHNVALYKAHIALEKPGAEDFDYESDAESIEEDVPRLDPEISREQVVELRTYGYWSEEAQSINLPSYIPT *F FVFLSGIPLQFMHEFLRMRLETRPVRPNPLSLEQLMKELREGLTLALTHRERYQRHITTALVENEAELGSYISILNHYDA *F TVRKTFELYLEYIDQLVLVAVPEGNQKSVLEKEWMFTKLISPMIKGMHTLASQKFCGIISKLLRSISERLVKRTVELDQQ *F IDGTADTDDNEEVKWQLLTICRETQSLLTVERERSIKVLFFAKTFCRDVETTDFHREHYEHDVANQQHDFICSDVKAAFK *F LLQQDVLQVRNKLTAIIEGVQKRCCLSNMRDLDEQDKQAVLSRTREILHQGYKFGFEYHKDVIRLFEQKIMDQKDSGAHT *F VDLALGIIAYAKMWMHFVMERCERGRGMRPRWASQGLEFLILACDPQITQHLDDDEFEALKQQMDRCISHVIGITSEPEK *F VAKKKASPRTRKTSSPATSRSRTPTRTPMSAGMVLNPNTPPLQSPPYNKLLHPQFSLKEDVSGTSYSPVDSSDYVDTPCQ *F RSANGELRLLVPQTPPTPASPGKSSLESTPLALRQERVRDAVNRLDMDLEDGLRERRLIGQVKSLNSSDKVHIRARSVHF *F RWHRGIKIGQGRFGKVYTAVNNNTGELMAMKEIAIQPGETRALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMEL *F CSEGTLESLVELTGNLPEALTRRFTAQLLSGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTTVPGEL *F QGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVGMGEKPQAPESLSQEGHDFID *F HCLQHDPKRRLTAVELLEHNFCKYGRDECSSEQLQMQVRGSFRRNVATSSS *F >CG11228.AA *F MSEPEVTSVVDMKSPNISSSCSFFKLKKLSEESLLQPPEKVFDIMYKLGEGSYGSVYKAVHKESSSIVAIKLVPVESDLH *F EIIKEISIMQQCDSPYVVRYYGSYFKQYDLWICMEYCGAGSVSDIMRLRKKTLTEDEIATILSDTLQGLVYLHLRRKIHR *F DIKAANILLNTEGYAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIEEIGYDCVADIWSLGITALEMAEGKPPYGEIH *F PMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKEPDDRATATELLEHEFIRNAKHRSILKPMLEETCAIREQQRANR *F SFGGVLAASQAKSLATQENGMQQHITDNAFMEDPGTLVPEKFGEYQQSSASDATMIAHAEQGVDEGTLGPGGLRNLSKAA *F APAAASSAASPLDMPAVDSGTMVELESNLGTMVINSDSDDSTTAKNNDDQKPRNRYRPQFLEHFDRKNAGDGLLMAYPLM *F NEQLIALNNQPNLLLSNAAPMGQQGIPAAAPAQPPPAYQNQHMHTQSHAYVEGEFEFLKFLTFDDLNQRLCNIDHEMELE *F IEQLNKKYNAKRQPIVDAMNAKRKRQQNINNNLIKI *F >fray.AA *F MTSIPANLSSNNVAGAATLPGAAAPPEKYTWPNSKDDYELRDVIGVGATAVVHGAYCIPRNEKCAIKRINLEKWNTSMDE *F LLKEIQAMSSCFHENVVTYHTSFVVREELWLVLRLLEGGSLLDIIKHKMRTSNCKQGVFDEATIATVLKEVLKGLEYFHS *F NGQIHRDIKAGNILIGDDGTIQIADFGADIWSFGITAIEMATGTAPYHKYPPMKVLMLTLQNDPPTLDTGADDKDQYKAY *F GKTFRKMIVECLQKEPSKRPTASELLKHAFFKKAKDRKYLTQTLLQSGPSMETRVHKAAKRQPGASGRLHRTVTGEWVWS *F SEEEDNGGSATGSGTGDRKHPSSDSDSEDRPMNRLERADSSDSDREEPSPEITHSVSSATVTPGAPAAAGAGAAQELTAG *F IAQLPLPSEAAGEAPPVNLVLRMRNLRRELHDIRFEFVVGKDTAEGIATELVDAGLVDALDTQPMATHLDQLIAASATMK *F TITFQLSSGVQPGEVPDERSLVGYAQISITD *F >Pak3.AA *F MSFTKWFKKKGGDGGSISEIGAPTNFQRHFHVSRNQETGDLEGLPAPWVRLMNSQITRDEQDKNPDAAYHAVKYYNYSIK *F KKENEVFKPFITEDVIHEESKEIENYVNYKNKHKSQDPEKSDDDGSSTATETESSSGCGSSAGNSNSSSINDSSQQSTVP *F LDALEEVFKELKTNLEHRETLKAAPQEPPPVPPKKSPHTIPPKPQIKPKPRVTQKFSRTSDIRRDEDSDNQHKINTDTII *F IKPAVGAQDAGADDNPDETILRRSKEKRAQKTDAEIYVELRAICNSDDPRERYKTTQEVGKGASGIVFIAADLQNESQVA *F VKTIDMKNQSSKDLILTEIRVLKDFNHKNLVNFLDAYLLEPEDQLWVVMEYMDGGPLTDVVTETVMKERQIACVCRETLY *F AISFLHAKGIIHRDIKSDNVLLGMDGSVKVTDFGFCANIEGDEKRQTMVGTPYWMAPEVVTRKKYGKKVDIWSIGIMAIE *F MIEGQPPYLYETPLRALYLIAANGRPDIKSWDKLSPNLQDFLDRCLQVEVDRRATADELLSHPFLNDCSEVKALVPNIKA *F AKKVLRRNV *F >CG4527.AA *F MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHM *F VEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLK *F AGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSE *F MSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVV *F DDETEVSAPWKPMDPCAQHRTLVVQLLDEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAA *F ATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAV *F AETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTAS *F SIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVM *F PPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDES *F EVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQ *F EKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDE *F EEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQEL *F RELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQ *F EQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINR *F NFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRAL *F PKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNE *F KRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVTNREEFEDQEVKLPAHLRNIYNER *F SSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPNTPIMHKQQRSQMIGDQVLEEDEEQMLSEQMK *F RASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPSMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEAS *F GVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQ *F PQLRSSVKPGHSRANGSVVSADSAASSEDIYYDLYAAQYKLPRQLQQRLQQEVSPLIYPQCASQPFDTTQGPETGARRLE *F ETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG *F >CG5169.AA *F MSWTEKVDPELIFTKQERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIDDIQQEIMVLSQCDSPYVTKYYGSFLK *F GTKLWIIMEYLGGGSALDLMKAGSFEEMHIGIILREVLKGLDYLHSERKLHRDIKAANVLLSEQGDVKLADFGVAGQLTN *F TTSKRNTFVGTPFWMAPEVIKQSQYDAKADIWSLGITAIELAKGEPPNSELHPMRVLFLIPKNNPPQLTGSYTKSFKDFV *F EACLNKDPENRPTAKELLKYPFIKKAKKNAYLIDLIDRFKKWKVSKGDESETESENSDSDSDAKQGGSSQDEPWIMTVKG *F LHINSAPRAGVNSFQLQEHELTMQKLMQTTHSPAGGGGVQASPAANALSSSVSAVAASASSVSVITANEGVSSASALPPQ *F NSHVHNHNHNNINNNNIRNLSNKHATTTMLNAAPPPSSVTSSSSASELQQKRSSSKPELRQSRSAAPLDQVEDRRASLGL *F PLQSSVPTPSTAEQQQQRRSSQRESFHANNAALVASCSSSSNNHSHEEHVATNHASHVKHSGAGQRLVQTNSACLNNFLF *F PVLSDIQRKYSGGGSAGSGGSGGRNIGVGSDIGDLKSVFELAERSSPGVSDLFMKELIHMLVPGYSETRINTIMDRAIRN *F RK *F >Pak.AA *F MSSEEDKPPAPPVRLTSNRGGNERSGGGVGVGGGGLGGGGMGDVPPDMRPLPKEPDDSDRKKKTLKSKIKGSKPSHTDSK *F PNISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQAVLDVLKWFDNTTKQRPSSKYMTNAITTHS *F GSSLSRVSSSSPSSTPTDSELHGSNSGGNLIGVQLGSMTLGPNANNVAVAGQILGNHYQQQQQHLLQQQQPLLHQNHNQH *F HMGISQSHSYNFVGHTVSSSTSQHSSANEDDMLGPQHPQQQPPPPPVASRPERTKSIYTRPIEDLQPAIIPMPVAPATTP *F ATPLQNHRTPGGISAPAASPMMHNNATTTLDKNKNNANLYTPEPTVAQVSAGGPSSQVAGNQIAVPQAAVAPAATPNTRA *F ANAKKKKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMR *F ENKHPNVVNYLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLD *F GSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALYLIATNG *F KPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFLKLARPLASLTPLIMAAKEATKGN *F >CG7097.AA *F MAAAHSHHNANMLSSDISRRNPQDEYELIQKIGSGTYGDVYKAKRIQSNELAAIKVIKLEPSDDIQIIQQEIIMMRDCRH *F PNIIAYYGSYLRRDKLWICMEFCGGGSLQDIYQVTGPLTEVQIAYMCRETLKGLEYLHSMGKMHRDIKGANILLTEYGDV *F KLADFGVSAQITATINKRKSFIGTPYWMAPEVAAVERKGGYNQLCDIWACGITAIELAELQPPMFDLHPMRALFLMSKSG *F FKPPTLNNKDKWSPTFHNFIKTALTKNPKKRPTAERLLQHPFVQCEMSLRVAKELLQKYQSPNPQFYYYLDGDEESVAGV *F PQRIASKMTSRTNGVPAQNHTLKTGMTTNSTWNERSSSPETLPSDMSLLQYIDEELKLRATLPLNNDTKDPLGAECSCSS *F HNGGAAGGGGGGGVGVGAGGAAANGSSSSSGGATVGTTHHQHQQHHQHHHHPNHLHQHQSHQLPQQQQQQSHQAAQQEHH *F HHHPMTSSISSGLVTANANNISSSASLASMPGLSAYLGMRSHVVGHMGMGFGMGLGMSNLRTTSIDADDDELVAADVAMN *F NAAAAVPGNGSGAGNGSGSGCSASAAHYLRYSSNYRSAAAAQASQAAHPHVNSNSNSNNGHGHAPITSSISSSASSASFY *F NRLLLLDNSSGDAVVGGNGGGSSNISSSGGASSGTNGLLDKHELDALPQAATKSAGDGFSHSNSPTANSGAGATGSRDND *F GPSSSNSSHLYQNLLRSSSGETPAGSSSAGNNCDYRHENNQNGLEDSPRRHSSMDQLIGLLENMGKSPRTRSLSDGGTQD *F DDEAEKEAQPDLLNNTPPVPPKRSHKRRHTPPRPISNGLPPTPKVHMGACFSKIFNGCPLRVHCTASWIHPETRDQHLLI *F GAEEGIYNLNMNELHDAAIDQLFPRRTTWLYVIKDVLMSLSGKSCQLYRHDLVALHSKQTVRFSLHMNKIPERLVPRKFA *F LTTKVPDTKCCTQCCVTRNPYNGYKYLCGATPSGIFLMQWYDPLNKFMLLKQCEWPAISIQGGGHGCVQNGHTPVFEMII *F TPELEYPIVCTGVRKAMNGCLKLELINMNSASWFHSEDLEYDAMATMVPRRDLLKVVRVHQVEKDAILVCYGNLIQVVTL *F QGNPKQHKKMVSQLNFDFNVDSIVCLPDSVLAFHKHGMQGKSLRNGEVTQEIKDMSRTYRLLGSDKVVALESQLLRTGSL *F GSEEGHDLYILAGHEASY *F >ninaC.AA *F MMYLPYAQLPDPTDKFEIYEEIAQGVNAKVFRAKELDNDRIVALKIQHYDEEHQVSIEEEYRTLRDYCDHPNLPEFYGVY *F KLSKPNGPDEIWFVMEYCAGGTAVDMVNKLLKLDRRMREEHIAYIIRETCRAAIELNRNHVLHRDIRGDNILLTKNGRVK *F LCDFGLSRQVDSTLGKRGTCIGSPCWMAPEVVSAMESREPDITVRADVWALGITTIELADGKPPFADMHPTRAMFQIIRN *F PPPTLMRPTNWSQQINDFISESLEKNAENRPMMVEMVEHPFLTELIENEDEMRSDIAEMLELSRDVKTLYKEPELFVDRG *F YVKRFDEKPEKMYPEDLAALENPVDENIIESLRHRILMGESYSFIGDILLSLNSNEIKQEFPQEFHAKYRFKSRSENQPH *F IFSVADIAYQDMLHHKEPQHIVLSGESYSGKSTNARLLIKHLCYLGDGNRGATGRVESSIKAILMLVNAGTPVNNDSTRC *F VLQYCLTFGKTGKMSGAVFNMYMLEKLRVATTDGTQHNFHIFYYFYDFINQQNQLKEYNLKADRNYRYLRVPPEVPPSKL *F KYRRDDPEGNVERYREFENILRDIDFNHKQLETVRKVLAAILNIGNIRFRQNGKYAEVENTDIVSRIAELLRVDEKKFMW *F SLTNFIMVKGGIAERRQYTTEEARDARDAVASTLYSRLVDFIINRINMNMSFPRAVFGDTNAIIIHDMFGFECFNRNGLE *F QLMINTLNEQMQYHYNQRIFISEMLEMEAEDIDTINLNFYDNKTALDNLLTKPDGLFYIIDDASRSCQDQDLIMDRVSEK *F HSQFVKKHTATEISVAHYTGRIIYDTRAFTDINRDFVPPEMIETFRSSLDESIMLMFTNQLTKAGNLTMPFEAVQHKDES *F ERKSYALNTLSAGCISQVNNLRTLAANFRFTCLTLLKMLSQNANLGVHFVRCIRADLEYKPRSFHSDVVQQQMKALGVLD *F TVIARQKGFSSRLPFDEFLRRYQFLAFDFDEPVEMTKDNCRLLFLRLKMEGWALGKTKVFLRYYNDEFLARLYELQVKKV *F IKVQSMMRALLARKRVKGGKVFKLGKKGPEHHDVAASKIQKAFRGFRDRVRLPPLVNEKSGQLNENTADFIRPFAKKWRE *F KSIFQVLLHYRAARFQDFVNLSQQVHIYNQRMVAGLNKCTRAVPFERINMREVNSSQLGPLPVPIKKMPFRLDQIPFYDT *F QYMVDPANSISRQAFPNQLLTQHMEDDEPWDSPLQRNPSMTSCALTYNAYKKEQACQTNWDRMGESDNIYNQGYFRDPQQ *F LRRNQMQMNMNAYNNAYNSYNSNYNNQNWGVHRSGSRRNSLKGYAAPPPPPPPMPSSNYYRNNPNQQQRNYQQRSSYPPS *F DPVRELQNMARNEGDNSEDPPFNFKAMLRKTNYPRGSETNTYDFNNRRGSDSGDQHTFQPPKLRSTGRRYQDDEGYNSSS *F GNYGVSRKFGQQQRAPTLRQSPASVGRSFEDSNARSFEEAGSYVEEEIAPGITLSGYAVDI *F >msn.AA *F MAHQQQQQLAPSVNCSLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVL *F KKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREILRGLSYLHSNKVIHRD *F IKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDENPDATYDNRSDLWSLGITALEMAESQPPL *F CDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDTVLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRK *F QEKEREDYRYSGSDNDDDEPQLAGEHSSIVQAPGGDTLRRNFQQIQEGRLAAEQQQQHHLMAQAQAQAAAAHAAAQAQAQ *F LQQQQQQAAAAAAAAAHAAQQAQQAAQQAQAQQPQANRQPKPPSRQQVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKC *F GEPAGQTPQQQQRNSQNNFKPSLPPRRPEDHLDVLAAQLSELGVVFSQQPQPQTAAGGQGSQQQAQPEAPPRNNRQSSGL *F SSSGGSASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKPLPGLGPVSEDAN *F TTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTSSVLPDLLSQ *F ASPATPPRHDKSSSEEKQRSFLTFGFGAGGSGPSRRESHVNVNVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLL *F IGTENGLMLLDRSGQGKVYQLISRRRFQQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINV *F GDLQGAVHFKIVKYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGFHAV *F DLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWGEMPTSVAYIGTGQIMGWG *F NKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSCQIYFMTLNKPGMANW *F >CG14217.AA *F MPSARPGSLKDPEIADLFNKHDPEKIFEDLREIGHGSFGAVYYARCNLTREIVAIKKMSYTGKQSQEKWQDILKEIRFLR *F QLNHPNTIEYKGCYLRESTAWLVMEYCVGSASDIIEVHKKPLHEDEIAAICLGVLSGLSYLHSLGRIHRDIKAGNILLTD *F NGVVKLADFGSAAIKCPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWSLGITCIELAERKPPYFNMNAMSALYHIAQNE *F SPTLPKNDWSDAFCSFVELCLKKMPAERPSSAKLLTHAYVTRPRSDTVLLELIARTKSAVRELDNLNYRKMKKILMVDTC *F ETESAVGDTDDQQDDHAGGDSSKSNSITSEHSIHSVGVSAASSQSSSSNSIPAAAQNHHHIAAHHHQQAASAAVAAAMHH *F HHHPHQQPPPSWPSGQQGQPVPPGAVSRNSSRHRNRPPLPNIMHSMNNNVTPTNSASVVPAPAPAPVLPPPISVLPHLSA *F MGHVGGGGTGTGGSGGGSPASGGPLADRIQPVQPRYLTTPAAQAAVYAASSASSQQAISNAVNDHGPNNFATIRTTSIVT *F KQQKEHMQEEMHEQMSGYKRMRREHQAHLVKLEEKCKVDMEAHKTALDKEYDTLLHNFTRDLDRLETKHQQDVERRAKQT *F SAAEKKLHKEITLKQENDRKVYDLNRKKEYKANKERWKRELSMDESTPKRQRDLTLQSQKDNLKQHEAQEEQRMLQAQKQ *F YIELEMRKFKRKRMIMQHEHEDQQLRDELGKKEQQLQQAHAMLLKHHEKTQELEYRQQKSVHQLREEQINKQHDTELHNQ *F KDYMDRIKKELVRKHAVELRQQPKSLKQKELQIRKQFRETCKTQTKQYKRYKAQVLQTTPKEQQKEVIKQLKEEKHRKLT *F LLGEQYEQSIADMFQSQSYKLDESQVIECQRTHEQLEYELEMLTAYQNKNKKQAQEQRDRERRELENRVSVRRGLLENKM *F DAELQQFNQERAERLRMKHEKHTKELEAFDNESIALGFSTLSLIEVSREAYADEEGSLSGSMISLAHSNSSTSFPAGSL *F >STLK.AA *F MMCSNNISDYKLLEILKNGMIGTVYKAEDINNKCLAVKKVSMDQPMEKLTLLFNEVLTVRRLQHRNINTIVSCFLYKQYV *F YLTYKFMCFGNCEVLLKNVYTSGFPEVAIALILKDVLSALTYIHSEHYVHGSVRAKHILLSPRKAVLSNFSYCQSFISQG *F EKKTFIFGSTVGIEKELYWTAPEVLYQNLSGYTEKIDIYSIGITCCEMANGFQPFKDTELTYMYIEKVRGSLQVLLDKNS *F LLENQGSLSLEHTNKRIARDVIVNKSFSENFHQFVELCLNKNPLSRWAASKLMTHSFLKQCRNTSLLDQLKDLGQKMSKF *F KRNERKYACTDNI *F >mbt.AA *F MFSKKKKKPLISMPSNFEHRVHTGFDKRENKYVGLPLQWASIVGNNQILKSSNRPLPLVDPSEITPTEILDLKTIVRPHH *F NNNKADTTSLNSSSTMMMGSMAPMNPMAPGAHPMMSHGPGMMMPPETGGIVLPKTSHVARSNSLRSSSPPRVRRVANVPP *F SVPEEEGPPAAGTPGVGGASSGGFKPPGAHPSLLYNSQHAHANGATGPLAVRTDQTNLQQYRSNLAPPSGGSMPQQQQTS *F PVGSVASGTRSNHSHTNNGNSGGSYPPMYPTSHQQQQQQQQQAKQGGDQNQNPLHPHAHPHPHHHQHLAKSASRASSSSG *F GASSAAQQASGASGGAAGQPKQDQRLTHEQFRAALQMVVSAGDPRENLDHFNKIGEGSTGTVCIATDKSTGRQVAVKKMD *F LRKQQRRELLFNEVVIMRDYHHPNIVETYSSFLVNDELWVVMEYLEGGALTDIVTHSRMDEEQIATVCKQCLKALAYLHS *F QGVIHRDIKSDSILLAADGRVKLSDFGFCAQVSQELPKRKSLVGTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEP *F PFFNEPPLQAMRRIRDMQPPNLKNAHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFLRQAGPPSLLVPLMRNARHHP *F >Dsor1.AA *F MSKNKLNLVLPPVNTEATVAAATVAPTPPFKTPSGTDTHSLLGKPKTSIDALTETLEGLDMGDTERKRIKMFLSQKEKIG *F ELSDEDLEKLGELGSGNGGVVMKVRHTHTHLIMARKLIHLEVKPAIKKQILRELKVLHECNFPHIVGFYGAFYSDGEISI *F CMEYMDGGSLDLILKRAGRIPESILGRITLAVLKGLSYLRDNHAIIHRDVKPSNILVNSSGEIKICDFGVSGQLIDSMAN *F SFVGTRSYMSPERLQGTHYSVQSDIWSLGLSLVEMAIGMYPIPPPNTATLESIFADNAEESGQPTDEPRAMAIFELLDYI *F VNEPPPKLEHKIFSTEFKDFVDICLKKQPDERADLKTLLSHPWIRKAELEEVDISGWVCKTMDLPPSTPKRNTSPN *F >lic.AA *F MSKRHRLTPFTIAKEPEAAIVPPRNLDSRATIQIGDRTFDIDADSLEKICDLGRGAYGIVDKMRHKQTDTVLAVKRIPMT *F VNIREQHRLVMDLDISMRSSDCPYTVHFYGAMYREGDVWICMEVMSTSLDKFYPKVFLHDLRMEESVLGKIAMSVVSALH *F YLHAQLKVIHRDVKPSNILINRAGQVKICDFGISGYLVDSIAKTIDAGCKPYMAPERIDPQGNPAQYDIRSDVWSLGIGM *F IEMATGRYPYDNWRTPFEQLRQVVEDSPPRLPEGTFSPEFEDFIAVCLQKEYMARPNYEQLLKHSFIVEHLQRNTDISEF *F VARILDLPDAQPAQ *F >Mkk4.AA *F MAERPKNLFATGSSRSRNPPDQLSLNNLSIRHPPSSTSSTSSGSTSSGSSSSSQHNHVTRCFGAQQPQQTPPVASSQVPP *F VPAASSSSAADRHRERIRQQACGKLQFGEGGANTHTFTSDDLEDEGEIGRGAFGAVNKMTFKKLDKVMAVKRIRSTVDEK *F EQKQLLMDLEVVMKSNECIYIVQFYGALFKEGDCWICMELMDTSLDKFYKYIYEKQQRHIPESILAKITVATVNALNYLK *F EELKIIHRDVKPSNILLHRRGDIKLCDFGISGQLVDSIAKTKDAGCRPYMAPERIDPERAKGYDVRSDVWSLGITLMEVA *F TGNFPYRKWDSVFEQLCQVVQGEPPRLLTSYNGMEFSKEFVDFVNTCLIKKESDRPKYSRLLEMPFIRRGETSHTDVAVY *F VADILESMEKDGITQFTANQQAES *F >hep.AA *F MSTIEFETIGSRLQSLEAKLQAQNESHDQIVLSGARGPVVSGSVPSARVPPLATSASAATSATHAPSLGAGSVSGSGISI *F AQRPAPPVPHATPFGSASASSSSSSASAFASAAPATGTFGGTYTPPTTRVSRATPTLPMLSSGPGGGLNRTRPVILPLPT *F PPHPPVSETDMKLKIIMEQTGKLNINGRQYPTDINDLKHLGDLGNGTSGNVVKMMHLSSNTIIAVKQMRRTGNAEENKRI *F LMDLDVVLKSHDCKYIVKCLGCFVRDPDVWICMELMSMCFDKLLKLSKKPVPEQILGKVTVATVNALSYLKDKHGVIHRD *F VKPSNILIDERGNIKLCDFGISGRLVDSKANTRSAGCAAYMAPERIDPKKPKYDIRADVWSLGITLVELATARSPYEGCN *F TDFEVLTKVLDSEPPCLPYGEGYNFSQQFRDFVIKCLTKNHQDRPKYPELLAQPFIRIYESAKVDVPNWFQSIKDNRLRA *F NGDPTLQRLPNS *F >tkv.AA *F MGSEFSANVQPDVEAMAAAALSGMEMGSGPGSEGYEDADNEKSKTVENARSLTCYCDGSCPDNVSNGTCETRPGGSCFSA *F VQQLYDETTGMYEEERTYGCMPPEDNGGFLMCKVAAVPHLHGKNIVCCDKEDFCNRDLYPTYTPKLTTPAPDLPVSSESL *F HTLAVFGSIIISLSVFMLIVASLCFTYKRREKLRKQPRLINSMCNSQLSPLSQLVEQSSGSGSGLPLLVQRTIAKQIQMV *F RLVGKGRYGEVWLAKWRDERVAVKTFFTTEEASWFRETEIYQTVLMRHDNILGFIAADIKGNGSWTQMLLITDYHEMGSL *F HDYLSMSVINPQKLQLLAFSLASGLAHLHDEIFGTPGKPAIAHRDIKSKNILVKRNGQCAIADFGLAVKYNSELDVIHIA *F QNPRVGTRRYMAPEVLSQQLDPKQFEEFKRADMYSVGLVLWEMTRRCYTPVSGTKTTTCEDYALPYHDVVPSDPTFEDMH *F AVVCVKGFRPPIPSRWQEDDVLATVSKIMQECWHPNPTVRLTALRVKKTLGRLETDCLIDVPIKIV *F >put.AA *F MSKYDLLYLTAQLTLVCCLIGIHGSILPGSHGIIECEHFDEKMCNTTQQCETRIEHCKMEADKFPSCYVLWSVNETTGIL *F RIKMKGCFTDMHECNQTECVTSAEPRQGNIHFCCCKGSRCNSNQKYIKSTTEATTQVPKEKTQDGSNLIYIYIGTSVFSV *F LMVIVGMGLLLYRRRKQAHFNEIPTHEAEITNSSPLLSNRPIQLLEQKASGRFGDVWQAKLNNQDVAVKIFRMQEKESWT *F TEHDIYKLPRMRHPNILEFLGVEKHMDKPEYWLISTYQHNGSLCDYLKSHTISWPELCRIAESMANGLAHLHEEIPASKT *F DGLKPSIAHRDFKSKNVLLKSDLTACIADFGLAMIFQPGKPCGDTHGQVGTRRYMAPEVLEGAINFNRDAFLRIDVYACG *F LVLWEMVSRCDFAGPVGEFQLPFEAELGLRPSLDEVQESVVMKKLRPRLLNSWRAHPGLNVFCDTMEECWDHDAEARLSS *F SCVMERFAQLNKYPSTQLLIKNHTNIDDAKESTNCL *F >SAX.AA *F MESNIYLVFLLTFLLYNNARAEISDHLREDIPDLDMELSSASSAHLNGKELPAVPQNSVQTHPRYKCYSCEPPCRDPYEF *F THTCQNAIQCWKSRTRDADGQVQESRGCSTSPDQLPMICSQNSLKINGPSKRNTGKFVNVVCCAGDYCNEGDFPELLPFD *F SNDVTVITADTSSISKMLVAVLGPFLVIALLGAVTIFFIRRSHRKRLAASRTKQDPEAYLVNDELLRATSAGDSTLREYL *F QHSVTSGSGSGLPLLVQRTLAKQVTLIECIGRGKYGEVWRGHWHGESIAVKIFFSRDEESWKRETEIYSTILLRHENILG *F FIGSDMTSRNSCTQLWLMTHYYPLGSLFDHLNRNALSHNDMVWICLSIANGLVHLHTEIFGKQGKPAMAHRDLKSKNILV *F TSNGSCVIADFGLAVTHSHVTGQLDLGNNPKVGTKRYMAPEVLDESIDLECFEALRRTDIYAFGLVLWEVCRRTISCGIA *F EEYKVPFYDVVPMDPSFEDMRKVVCIDNYRPSIPNRWSSDSLMTGMSKLMKECWHQNPDVRLPALRIKKTIHKLASADEK *F IRLDFDEVCV *F >wit.AA *F MNWAIYLLLALISLGRATPVPNRQYSCMSYQEDDNSFHDDDGDQDSSGELQEQQVESTPIPSEPHRRTCPDGYTFCFTIW *F NQTANGARVVKQGCWKDNTDRTSICSQSECTSSAPTSKTSSLYYCCCSGGVCNAQYSVVEPAPLELGSNEGRTSITNRAT *F EKQHQSFLASTMLGLAGGLTALTIGIFLAVQYCRTAKEKPEPEESPLAPSGPGYSSNLRNVDNMNLIGMLGSGKYGTVMK *F GLLHDQEVAVKIYPEEHHQYYVNERNIYALPLMECPALLSYFGYDERCTMDGRMEYQLVLSLAPLGCLQDWLIANTLTFS *F ECCGMLRSITRGISHLHTELRLGDQHKPCVAHRDINTRNVLVQADLSCCIADFGFALKVFGSKYEYKGEVAMAETKSINE *F VGTLRYMAPELLEGAVNLRDCETSLKQMDVYALGLVLWEVATRCSDFYAPGQATPPYKAPYEQEVGSHPSFDQMQALVVR *F HKARPLFPTGWGGGAAAKVVRDTCEDCWDHDADARLTSLCAEERMQEMSTLRPRAQAQPSSPLLNTNNLVASPTAEQGIN *F IIATTTTAAAVHHQMSSDTTGLIQPPPNQQIPLAALEREKNHLSYPQQQLQPYQGRNPCQERNLAPLTMRTPPVLVERSK *F KHSFQTQPQENSLSCLEHDVSVEELIASHQHQQQKNTIVSTGGNGNSCLGQGFPKQQNTDQKLRGWHGVRALIHKKLFRK *F EHAEELCRQLQLGEEKSNLVTALRRPNNLDLNPRLDKPPPDQLRSAEQRMGTPAHIVPRSLSSSLIKHINGTTNNNSIQS *F HGSELQTLTRPASKRRPGHLRTNSLMATTGQGPPTEQQMRRQHSLEVFREVFSGRGSSERLRDPSERVKTPGDVPPSVRK *F ARASKTLSLYDDRMMDSSLLNIL *F >BABO.AA *F MLSALRLIFLGALLGASVCASPIEFVMDTSLNGSRSDPATATHPGKWPPTTKAPALRAPAGTAGHAYQSPSSSLAADNRS *F HDNNNASAVSMLLPQDGDASGAVAPAVTPQLPIYIAQPSAKKPENKIKCHCDTCKESNNICETDGFCFTSVEKNSDGSII *F FSYSCMVVKYNMQRSKPFECLTSNERFDTYRIDCCKSDFCNKNEIMKRIFETDYVPHRLTSWEFVAIILGATLFICFTGT *F STWYYCQRRKRMASGRTFAKEDSAYDPILNGNTTIHDIIEMTTSGSGSAGLPLLVQRSIARQVQLCHVIGKGRFGEVWRG *F RWRGENVAVKIFSSREECSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVTDYHENGSLFDYLTTHPVDTNTM *F LNMSLSIATGLAHLHMDIVGTRGKPAIAHRDLKSKNILVKSNLSCAIGDLGLAVRHVEKNDSVDIPSTHRVGTKRYMAPE *F VLDESMNDQHFDSYKRADVYAFGLILWEIARRCNMGMIYDEYQLPYYDVVQPDPSIEEMKKVVCIEKCRPNIPNRWHASD *F VLHNMAKVMKECWYPNPVARLTALRIKKTLASISVEDKVKN *F >shark.AA *F MSRDSDPMKWYHGNLSREAADELLKQGYEDGTFLVRESSTAAGDFVLSLLCQGEVCHYQVRRHGGEDAFFSIDDKVQTKI *F LHGLDTLVDYYQQAANGLPTKLTVPLIRDLPPHNTRSHGVTNLLHRATSKNESKVVFELLKCGYRNFDAKNQDGQTALHL *F AALHSDEDILKHLLNAKVQVNSSDSFGCQPLHYAARSKPASFIRTLISAQANVQGRNIDNGYVPLHEAAKHGNLEAVQEL *F LLAEAPPLPRTSSGEFPFDLAKEAGQTAVEEFLLNYKLPPANTTRDQWYHGTLTREEAVAILKKHAKELLAKQPEVDTSG *F CFLVRYSESPAASGLVLTLLCDQVVKNFRISQADLYQNGNKVQSGGSKFLYIDDGPYWPSVEHLIAHFMRFSYGLPVSLK *F YPVPPQPKPEVPSFATIPRSNMKPKAASPATPPTPVSPHSHHQHPHVPALTITKKKQKENSSSMFNTLRLTSPKKALFDM *F NSLRKNKSKGKRSDSESSVSGSLAGTEQELQAAAPMLKSLSFSTEFSTFNADGVTGSGAAAAGEVYNVPRNNTPIEIDLP *F PIAQKTEAEVEYFTKSDVAIERERAGQWIGNGYQPTMDVLSLLDQQIKAPAVARLNSLGPNASTESEMASYLHRKCSGTP *F STPSATEVEAAKLRFFIEPEKLVLDREIGHGEFGSVHSGWLLRKSGAGEESRLEVAIKMLSDEHSNKQEFLREASVMMRL *F EHKCIVRLIGIAKGEMLMMVQELAPLGSMLQYILDHGHEITANAELKVWASQIACGMHYLESQHFVHRDLAARNILLTAR *F HQAKISDFGMSRSLRPGSTEYQFTQGGRWPIRWYAPESFNLGIFSHASDVWSFGVTIWEMFSLGAPPYGEISNVDAIKLV *F DSGERLPQPNLCPAYIYAVMQSCWKERPKDRPTFVYLTEFFARDPDYQNLPELVQTVHI *F >Taf250.AA *F MEMESDNSDDEGSIGNGLDLTGILFGNIDSEGRLLQDDDGEGRGGTGFDAELRENIGSLSKLGLDSMLLEVIDLKEAEPP *F SDDEEEEDARPSAVSASEGMSAFDALKAGVKREDGAVKAQDDAIDYSDITELSEDCPRTPPEETSTYDDLEDAIPASKVE *F AKLTKDDKELMPPPSAPMRSGSGGGIEEPAKSNDASSPSDDSKSTDSKDADRKLDTPLADILPSKYQNVDVRELFPDFRP *F QKVLRFSRLFGPGKPTSLPQIWRHVRKRRRKRNQSRDQKTTNTGGSDSPSDTEEPRKRGFSLHYAAEPTPAECMSDDEDK *F LLGDFNSEDVRPEGPDNGENSDQKPKVADWRFGPAQIWYDMLEVPDSGEGFNYGFKTKAASTSSQPQLKDERRVKSPEDD *F VEDPSIADDAFLMVSQLHWEDDVVWDGNDIKAKVLQKLNSKTNAAGWLPSSGSRTAGAFSQPGKPSMPVGSGSSKQGSGA *F SSKKAQQNAQAKPAEAPDDTWYSLFPVENEELIYYKWEDEVIWDAQQVSKVPKPKVLTLDPNDENIILGIPDDIDPSKIN *F KSTGPPPKIKIPHPHVKKSKILLGKAGVINVLAEDTPPPPPKSPDRDPFNISNDTYYTPKTEPTLRLKVGGNLIQHSTPV *F VELRAPFVPTHMGPMKLRAFHRPPLKKYSHGPMAQSIPHPVFPLLKTIAKKAKQREVERIASGGGDVFFMRNPEDLSGRD *F GDIVLAEFCEEHPPLINQVGMCSKIKNYYKRKAEKDSGPQDFVYGEVAFAHTSPFLGILHPGQCIQAIENNMYRAPIYPH *F KMAHNDFLVIRTRNNYWIRSVNSIYTVGQECPLYEVPGPNSKRANNFTRDFLQVFIYRLFWKSRDNPRRIRMDDIKQAFP *F AHSESSIRKRLKQCADFKRTGMDSNWWVIKPEFRLPSEEEIRAMVSPEQCCAYFSMIAAEQRLKDAGYGEKFLFAPQEDD *F DEEAQLKLDDEVKVAPWNTTRAYIQAMRGKCLLQLSGPADPTGCGEGFSYVRVPNKPTQTKEEQESQPKRSVTGTDADLR *F RLPLQRAKELLRQFKVPEEEIKKLSRWEVIDVVRTLSTEKAKAGEEGMDKFSRGNRFSIAEHQERYKEECQRIFDLQNRV *F LASSEVLSTDEAESSASEESDLEELGKNLENMLSNKKTSTQLSREREELERQELLRQLDEEHGGPSGSGGAKGAKGKDDP *F GQQMLATNNQGRILRITRTFRGNDGKEYTRVETVRRQPVIDAYIKIRTTKDEQFIKQFATLDEQQKEEMKREKRRIQEQL *F RRIKRNQERERLAQLAQNQKLQPGGMPTSLGDPKSSGGHSHKERDSGYKEVSPSRKKFKLKPDLKLKCGACGQVGHMRTN *F KACPLYSGMQSSLSQSNPSLADDFDEQSEKEMTMDDDDLVNVDGTKVTLSSKILKRHGGDDGKRRSGSSSGFTLKVPRDA *F MGKKKRRVGGDLHCDYLQRHNKTANRRRTDPVVVLSSILEIIHNELRSMPDVSPFLFPVSAKKVPDYYRVVTKPMDLQTM *F REYIRQRRYTSREMFLEDLKQIVDNSLIYNGPQSAYTLAAQRMFSSCFELLAEREDKLMRLEKAINPLLDDDDQVALSFI *F FDKLHSQIKQLPESWPFLKPVNKKQVKDYYTVIKRPMDLETIGKNIEAHRYHSRAEYLADIELIATNCEQYNGSDTRYTK *F FSKKILEYAQTQLIEFSEHCGQLENNIAKTQERARENAPEFDEAWGNDDYNFDRGSRASSPGDDYIDVEGHGGHASSSNS *F IHRSMGAEAGSSHTAPAVRKPAPPGPGEVKRGRGRPRKQRDPVEEDLQCSTDDEDDDEEEDFQEVSEDENNAASILDQGE *F RINAPADAMDGMFDPKNIKTEIDLEAHQMADESMDVDPNYDPSDFLAMHKQRQSLGEPSSLQGAFTNFLSHEQDDNGPYN *F PAEASTSAASGADLGMDASMAMQMAPEMPVNTMNNGMGIDDDLDISESDEEDDGSRVRIKKEVFDDGDYALQHQQMGQAA *F SQSQIYMVDSSNEPTTLDYQQPPQLDFQQVQEMEQLQHQVMPPMQSEQLQQQQTPQGDNDYAWTF *F >CG4041.AA *F MGTRERERECRLCAVTFFAKLHPGDVCGSNGLPLTPNSIAILGRAQKLKELQDEHLCQYLDVIRGKHERTIVVSEYLGLS *F LEDYAMRHPPLAIAQILRIFYQVACGINVLSRHHLVAHNVEPKHILLSSDGQRVKLFNYGLHHMTKGGAYVPFPIGNIRY *F MAPERLLGLNGNVKSDVWSLALVMVELILQIELWPKLKLSNVVRKILAFGKSNGALEKIAREHQCHERYVQMDQRLRQLL *F ESCLSVLPKRRPLPGELLEHPIFEEVLLDLKKQKMQPLSPETEHLPLLLRCPLSQIYHLWQLAGGDVQAELKKEGLIRSE *F APILGLPQIVRLSGASVCPGRSQAQLMDDRVVPLRLKALLQRLSGLPAAVYFPLLHSPRFPAHFARELQELPLVIREKDI *F EYQFQRVRLFARLLQGYPHTAEQLQREAAVDVPPLLRGPIWAALLEVVPNGSYAKIDKFTSTSTDRQIEVDIPRCHQYDE *F LLSSPDGHRKLRRLLKAWVTAHPQYVYWQGLDSLTAPFLYLNFNNEELAFLSLFKFIPKYLQWFFLKDNSAVIKEYLSKF *F SQLTAFHEPLLAQHLASISFIPELFAIPWFLTMFSHVFPLHKILHLWDKLMLGDSSYPLFIGIAILRQLRSTLLTSGFNE *F CILLFSDLPDIVMDGCVLESQKMYEATPKSITHRQHALRLQPPQALDIGVADVELKHLQQEQCPRISAKDVQFLLDNSPA *F ELALIDLRSVVEFGRVHVPHSINIPFATVQLGEQRLEALQVPQLEAQLRGKIVVCVSNIHQHSVEVGHPLAQLKLLCKLK *F SFPISQFSHFLVACGVQRTCILHKGFNVLHSIEPNILISN *F >Btk29A.AA *F MIPCVSLAETSVIGNMKERVKEMKVFGCRLNFWNHIGHSLTSSKTKEGNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSG *F SLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHF *F VKLVVALYPFKAIEGGDLSLEKQNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAES *F LLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSS *F PCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLV *F QLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVK *F VADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIIL *F EKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD *F >WSCK.AA *F MECGSHSGHRPIPIWLSSCLVAMCLGLPLGAAVPQEAPAYYYVGCYTARTDLLHESVYAKTPQTCIEICEHQGHHYAVLA *F SEKCFCANVLEPQEQQDEQLCNTRCLANKAQYCGGVGVHSYYSTILTKQPGPHHLRISNKTENSLTLSWNAYEARKLLLA *F GGAEAVLPNQLLDNFLIKAQVLKTYSSLPAFPQPEFMVQSTETQFELTDLHPATLYNISVRAMCKDAQVGQSECGQASIE *F ATTEVGLPSPVPAQPKILSRTDRTVTIELSPIRNDNGPLSKLLVIVEYVDNALSQPFDAQLLGSWQQAQQDGVPYYIAAE *F LDYDRPEDNRTRRFVVGDGKRYGRFTNKPLDQPDAHVHISLGLVSNALNSNEYLCNYLYSRXSTLEGVTKTMYSRGTHDQ *F HVTSLDDFSYATFEKGQSSVVALAVTCVIFGSCLLLSLIAYFYLRYKTCRGRRLTGGNTHEMTLQTPIIERENNGYLVED *F DPLPHSPENFKQQLQQLVEGYERIPRNALRLNVNDVIGDGRFGEIITGKVSTNDFARDCTLHVLCLDDLNGTTQAQLLRE *F LRQLSQLKRQEHLLDFYGVSASPDWFYLIFEQQRMSLKRKLVESRLMAPSPRLTSLSEQLVLQWIYELASAMNYLSSCQV *F VHRQLCSHSVFVTSDFKLKLSVFGPLPYMNIARQQPDHNRWLAPEVLRHQHHHSTRSDVWSLACVAWECCALGGTPYANA *F VASNQQLLEAIRAAVRPAQPAYVYGDLYQLLLNCWQLEPSERSSCEDVAFGVRQLMTSPRHALSFDRVAGGLDTLPPYLP *F QLEAVATMG *F >TOR.AA *F MLIFYAKYAFIFWFFVGSNQGEMLLMDKISHDKTLLNVTACTQNCLEKGQMDFRSCLKDCRINGTFPGALRKVQENYQMN *F MICRTESEIVFQIDWVQHSRGTEPAPNATYIIRVDAVKDDNKETALYLSDDNFLILPGLESNSTHNITALAMHGDGSYSL *F IAKDQTFATLIRGYQPSKMGAVNLLRFVPQPDDLHHIAAEIEWKPSAESNCYFDMVSYSTNSVNMDEPLEVQFRDRKKLY *F RHTVDNLEFDKQYHVGVRTVNIMNRLESDLQWLPIAVPSCLDWYPYNYTLCPPHKPENLTVTQKQYLPNILALNITWARP *F RYLPDNYTLHIFDLFKGGTELNYTLDQNRSHFYVPKITVLGSHFEVHLVAQSAGGKNVSGLTLDKVHRGVLLSEGNMVKL *F VLFIIVPICCILMLCSLTFCRRNRSEVQALQMDAKDAKASEFHLSLMDSSGLLVTLSANESLEVMDELEVEPHSVLLQDV *F LGEGAFGLVRRGVYKKRQVAVKLLKDEPNDEDVYAFKCEIQMLKAVGKHPNIVGIVGYSTRFSNQMMLLIEYCSLGSLQN *F FLREEWKFRQEQNAIGLKKNLEQNVDNRRFNRLPRNSIHDRIEDINNSMLSTVEEESESDQTHSSRCETYTLTRITNAAD *F NKGYGLEDIENIGGSYIPKTAEAPKDRPKRKLKPQPKKDSKQDFKSDNKKRIFENKEYFDCLDSSDTKPRIPLKYADLLD *F IAQQVAVGMEFLAQNKVVHRDLAARNVLISVDRSIKIADFGLSRDVYHENVYRKSGGSGKLPIKWLALESLTHQVYTSQS *F DVWSFGVLLYEITTLGGMPYPSVSPSDLLQLLRQGHRMKRPEGCTQEMFSLMESCWSSVPSHRPTFSALKHRLGGMILAT *F NDVPERLKQLQAATESKLKSCDGLNSKVEQVPCEEELYLEPLN *F >Cad96Ca.AA *F MVYHHHNHESRIIHCRKQLTSWRRRSLLLTIIVVTATVVSLISQEAEAHNQNAPPILYVRERNWRISETEKVGQIIDRVR *F AEDPDGDDLIFGIEPRFSLPGGENDASPPEKIPFQIDRETGVVTLNESLAGRAGQNFLIYITVTDGSYTAKNEVFINILG *F ERENSSGYRPQTSISNVVHNISQFLPRFDQLPGVQSIRNGLPNSRPGGWYPPVPQNNIFGPPPFGNNYPPPPPNIPGVRG *F EQSGEEEQPDEEVTPTTPVRISSTTPKSRTKLTPITANNSTRVESAIPAETTTPSGGHHNNSSSPITIFSLKSGTIPIVV *F TVGGFFVAIAVLLAYLCRRRLCAISRTLKKTKEKEELAKKSNQSQLSSTLTDDSRNSMVMQQWQGPVAFANRYVPWERDQ *F QMGIATSQLSTGVTNGGVSSPGVPSPGTGEPGSNLGPGCLTGGAGSSGAPENAFAGEANCDRWEFPRYRLKFFNILGEGA *F FGQVWRCEATNINGNEGITTVAVKTLKESATEVDRKDLLSELEVMKSLEPHINVVHLLGCCTDKDPTFVILEYVNRGKLQ *F TYLRSSRAERHYGNTHGKSNVLTSCDLTSFMYQVAKGMDYLTSRGIIHRDLAARNILITDDHTCKVADFGFARDVITSKI *F YERKSEGKLPIRWMATESLYDNIFSVKSDIWSFGILMWEIVTLGSTPYPGISAADVMRKVRDGYRLEKPEHCRRELYNIM *F YYCWSHDPQERPLFAEIIQMLDKLLHTEMDYIELERFPDHNYYNIVSLSGEKL *F >Tie.AA *F MKIHRGNESFCSCWLLLLLLVALLLAEAIVAAAAATLSETQTNATNGAIREPPVQLQLQQQQLQLQLQLNATAGDGESRN *F SAEIFSPGDVDVVGDKPTTYRPSGANRPSIAPLNSAQDRERKLMNVLAARRTALHRGGFRTRIGTTGRTAISPSAAVEST *F SKAPSDPRSVCIRNCTKNFTRRTTASCMRQCANFTRMAMPSNGNSSVVLKGSASLKAAAPAGDRDTNEILFSDESSHGLF *F VVAKEQNGTLNHIADGTKPAVMGKGIPSKTNSMEDDVEEEEDELKPYVYFGAKLPPIRPGGLTIKAAEGDDDHPRVLEVS *F VAQSTTPTITTTSTTPAPVSSTRPTASTRRPLTPVERSRSKYKYHMRERGLVPTAAPPPAAPPAPPVSRTRYVGSGRPTA *F GLSDSIQDLKQTESEVAKPVVRRVVQKVSPSSSTPLIITVLSAEEETTVGQTEALPTDESTTTTSTTTTTEKPSTTTSTS *F TTTTTPVPSTSKRTTTNAPLSTTNTPTTTTSSTTPSTTTTSSTSTTVIPTTTKANSIIEKDKELIRALGPALTREINIDD *F ANNLIVFCNNTSDCGVTPRTQTQPSHTTDSSPKILRTTVLTSIRSTVHPRPTSTSTTTSTTTTAPPAVTAPSNEVYIGIV *F ESSSSASPADISSTVIANERDLNMEMRRMNLVTLVLVAVGVIPLAAIILYLVRNFVIRRRAKQSEVFDVCITDQQPISPV *F KKVDSKYQVDDDEDEVDHQHHQHMQHHQNHQNHQNHQHHQAMPMSQASQRDANHNRYGNNDDKTSLASEFHDFERSNIRL *F KSLLGEGNFGQVWKAEADDLSGHFGATRIVAVKTIRACSAQVSLKDEANIMRKLGSHQNVVTLLGACVESEPHMLIMEYA *F MRGRLLSLLRAARSATNILPASVPGGRSLAPLSPRTLAGFALDIACGMEYIAGRRIVHRDLAARNVLLDHNGMCKICDFG *F MSIDLDAERMRKEQEKNAANDLMRHNAHKFKFDFGSRYILQHWQHTFGQGQGQGHCSKDQPHGEKKSHHGHDTIGKRHAL *F PIRWMAPESLQYHMFTTETDIWAFGIVLWEIATLGSTPYSQLTGREVIRRVPQGLRPDLPKESRHEFYNLMSRCWHKEPH *F MRPSFAQSRLEITRSLHKWVDDDSAASDYMDVSGFSEDLEHGVVYFNHRISEFECEI *F >BcDNA:GH07910.AA *F MSPGAHLQMSPQNTSSLSQHHPHQQQQLQPPQQQQQHFPNHHSAQQQSQQQQQQEQQNPQQQAQQQQQILPHQHLQHLHK *F HPHQLQLHQQQQQQLHQQQQQHFHQQSLQGLHQGSSNPDSNMSTGSSHSEKDVNDMLSGGAATPGAAAAAIQQQHPAFAP *F TLGMQQPPPPPPQHSNNGGEMGYLSAGTTTTTSVLTVGKPRTPAERKRKRKMPPCATSADEAGSGGGSGGAGATVVNNSS *F LKGKSLAFRDMPKVNMSLNLGDRLGGSAGSGVGAGGAGSGGGGAGSGSGSGGGKSARLMLPVSDNKKINDYFNKQQTGVG *F VGVPGGAGGNTAGLRGSHTGGGSKSPSSAQQQQTAAQQQGSGVATGGSAGGSAGNQVQVQTSSAYALYPPASPQTQTSQQ *F QQQQQPGSDFHYVNSSKAQQQQQRQQQQTSNQMVPPHVVVGLGGHPLSLASIQQQTPLSQQQQQQQQQQQQQQLGPPTTS *F TASVVPTHPHQLGSLGVVGMVGVGVGVGVGVNVGVGPPLPPPPPMAMPAAIITYSKATQTEVSLHELQEREAEHESGKVK *F LDEMTRLSDEQKSQIVGNQKTIDQHKCHIAKCIDVVKKLLKEKSSIEKKEARQKCMQNRLRLGQFVTQRVGATFQENWTD *F GYAFQELSRRQEEITAEREEIDRQKKQLMKKRPAESGRKRNNNSNQNNQQQQQQQHQQQQQQQNSNSNDSTQLTSGVVTG *F PGSDRVSVSVDSGLGGNNAGAIGGGTVGGGVGGGGVGGGGVGGGGGRGLSRSNSTQANQAQLLHNGGGGSGGNVGNSGGV *F GDRLSDRGGGGGGIGGNDSGSCSDSGTFLKPDPVSGAYTAQEYYEYDEILKLRQNALKKEDADLQLEMEKLERERNLHIR *F ELKRILNEDQSRFNNHPVLNDRYLLLMLLGKGGFSEVHKAFDLKEQRYVACKVHQLNKDWKEDKKANYIKHALREYNIHK *F ALDHPRVVKLYDVFEIDANSFCTVLEYCDGHDLDFYLKQHKTIPEREARSIIMQVVSALKYLNEIKPPVIHYDLKPGNIL *F LTEGNVCGEIKITDFGLSKVMDDENYNPDHGMDLTSQGAGTYWYLPPECFVVGKNPPKISSKVDVWSVGVIFYQCLYGKK *F PFGHNQSQATILEENTILKATEVQFSNKPTVSNEAK *F >CG8173.AA *F MDTPRRKLRNLHLENVQNSSTPINVPPSPMMKTLGHGTGIRVYRLDRSPRLGEIRSPWAVKRITQNMRVKKDTLFNERIV *F HEADILRKLKHPNIVGFRGVITNDEGINTLALEMCTTSLGSILEERHDEDLGPLPAKHTYKMIMDVAQALDFLHNEAHLM *F HGDLKSFNVLVKGEFEICKLCDFGVSLPLDEQGEVNFLKNPGLRYVGTNLWCAPEVIDEVDVIDSKADIFSFGLVIYETL *F ALVPPHTLELDAALGEDMDSSHDLPTDTDKLQCKQLDFSSDENKNGLPSAMEEHTDNDMSQDYDEEDDEEEKEEEEEDDE *F EDDDTKENDISYFTLNNLHSAYGTRPPLPVAFQLSDDYNCVVELFYLCTNALSEDRPAAKTIWQCLENNGANVATESD *F >trbl.AA *F MDNSSGQNSRTASSASTSKIVNYSSPVSPGVAAATSSSSSSSSSGMSSSQEDTVLGLFTPKKEFPNAKMLQTIREKLMTP *F GGACDLLALGIAAEPTDQQPVKLIQQRYLISAQPSHISAAVAAKTPASYRHLVDLTASNLRCVDIFTGEQFLCRIVNEPL *F HKVQRAYFQLQQHDEELRRSTIYGHPLIRPVHDIIPLTKDRTYILIAPVPQERDSTGGVTGVYENLHTYIRHAKRLCETE *F ARAIFHQICQTVQVCHRNGIILRDLKLKRFYFIDEARTKLQYESLEGSMILDGEDDTLSDKIGCPLYTAPELLCPQQTYK *F GKPADMWSLGVILYTMLVGQYPFYEKANCNLITVIRHGNVQIPLTLSKSVRWLLLSLLRKDYTERMTASHIFLTPWLREQ *F RPFHMYLPVDVEVAEDWSDAEEDEGTAADAMDDDEEGLCPLGDKHEYEDIGVEPLDYTRSTLQMAQNANGLSTEPEPDTD *F VDMG *F >CG14305.AA *F MSKYNSSAGIRQLGTRSSDVDALAQRGYNVGHKIGEGSYATVITAGYADDHGHGVHLACKIIDKAKAPTDFVNKFFPREL *F EILTKIDHSNIIQIHSILQRGPKIFIFMRYAENGDLLSHIKRSGPIDEKQSKIWFFQMSKALKYLHNLDIAHRDLKCENI *F LLSKRLNIKLADFGFARYCRDDNGREMKSETYCGSAAYAAPEVVCGRPYDPKLADAWSLGVILFIMMNAKMPFDDSNLTK *F LLEDQRNRKFAFRRKLQETISAQAKATVSVLLEPEAHARWNLREILNCAWLRTVEESQTPIP *F >CG9222.AA *F MRFPLVIGASSSQDLVTDQNSGRRQEQKVYTFSDRPPQPKPPAPSGVVPVKADDKSKPQKTILEEHGIILGKVIGTGNYA *F KVKIGFSEEYGKRVAVKIISKVKAPSEYTQKFLPREIEAVKGLHHENLITFYQSIETSHRVYLIMQLAENGTLLDYVRER *F KFLDEPQSRTLFKQLVSAVEYIHSKGVVHRDIKCENLLLDENWNLKLIDFGFARKDTRTSDNQVILSKTFCGSYAYASPE *F ILKGVAYDPFMSDIWACGVVCYAMVFGRLPYDGSNVHILLKRINQSLVFPKSPSASSECKHMIMHILAPVKIRYNIPQVK *F EDPWYSPSK *F >CG11533.AA *F MTSEDLLQPGHVVKERWKVVRKIGGGGFGEIYEGQDLITREQVALKVESARQPKQVLKMEVAVLKKLQGKEHVCRFIGCG *F RNDRFNYVVMQLQGKNLAELRRAQPRGAFSLSTTLRLGLQILKAIESIHSVGFLHRDIKPSNFSVGRLPYNCRRVYMLDF *F GLARQYTTGTGEVRCPRAAAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVNGQLPWRKIKDKEQVGLTKEKYDH *F RILLKHLPSDLKQFLEHIQSLTYGDRPDYAMLIGLFERCMKRRGVKESDPYDWEKVDSTAIGNISATGNPSIPIKSDYMH *F GNITQMTVAASNASGTEYIRKRAEIETAHITATDPLNIKEKVDK *F >CG7643.AA *F MTTPVPRRTKDMMALGLDSDSEDDFNTPYRPRQAAAGERKQQPVASFQVQTRGKENEPHPLPINMLPRRVSELTMMDSDS *F DEEDIKSNHNLNCAILNDSFVLSPSQELTNSNSNITTRRTPSAAPLKQSDSNLSFLGRFNDMGINCSSQGSPVANSEKQV *F AKKTAPTLQAAPSATERRPLQETETPLRNELPSTSKTKPDADFITPQVRTIGSTLAGKSRSAVSNDFRAQKVLFQTPMTV *F SRAAPVASDSISFSLCDTITESPDIPEPPKKAEPPKSQHPSKKSLDHVFRESDKDNVPDKVDNVEPKELVSIPAVAVPPE *F QPSHKTSNILKIKNHEYTIDKKLGCGGSSSVFLARRSDSGNEFALKVVDLQADPQVVQGYLNETKLLAKLQGNVCVVALY *F DYQLVREESKLYMVMEKGDCDLNKILQSYTTNLPLYSLMNILYQMLQAVNYIHQHGVIHSDLKPANFLMVSGRLKLIDFG *F IASNIAVDSTSIIKFSQAGTFNYISPEALTDTSTGNSPMRRADQPKIKISTKSDVWSLGCILYLLLYQKTPFGHIRNVYA *F KMSAITTPGTSIEYPAIPPYYPIMLVHMAKNCLQLNPKKRPSCTELLQYPFHMIIPLQNLQIPSRTANSN *F >fu.AA *F MNRYAVSSMVGQGSFGCVYKATRKDDSKVVAIKVISKRGRATKELKNLRRECDIQARLKHPHVIEMIESFESKTDLFVVT *F EFALMDLHRYLSYNGAMGEEPARRVTGHLVSALYYLHSNRILHRDLKPQNVLLDKNMHAKLCDFGLARNMTLGTHVLTSI *F KGTPLYMAPELLAEQPYDHHADMWSLGCIAYESMAGQPPFCASSILHLVKMIKHEDVKWPSTLTSECRSFLQGLLEKDPG *F LRISWTQLLCHPFVEGRIFIAETQAEAAKESPFTNPEAKVKSSKQSDPEVGDLDEALAALDFGESRQENLTTSRDSINAI *F APSDVEHLETDVEDNMQRVVVPFADLSYRDLSGVRAMPMVHQPVINSHTCFVSGNSNMILNHMNDNFDFQASLRGGGVAA *F KPIVAPTVRQSRSKDLEKRKLSQNLDNFSVRLGHSVDHEAQRKATEIATQEKHNQENKPPAEAISYANSQPPQQQPQQLK *F HSMHSTNEEKLSSDNTPPCLLPGWDSCDESQSPPIENDEWLAFLNRSVQELLDGELDSLKQHNLVSIIVAPLRNSKAIPR *F VLKSVAQLLSLPFVLVDPVLIVDLELIRNVYVDVKLVPNLMYACKLLLSHKQLSDSAASAPLTTGSLSRTLRSIPELTVE *F ELETACSLYELVCHLVHLQQQFLTQFCDAVAILAASDLFLNFLTHDFRQSDSDAASVRLAGCMLALMGCVLRELPENAEL *F VERIVFNPRLNFVSLLQSRHHLLRQRSCQLLRLLARFSLRGVQRIWNGELRFALQQLSEHHSYPALRGEAAQTLDEISHF *F TFFVT *F >CG10967.AA *F MNIVGEYEYSSKDMLGHGAFAVVYKGRHRKKHMPVAIKCITKKGQLKTQNLLGKEIKILKELTELHHENVVALLDCKESQ *F DCVSLVMEYCNGGDLADYLSVKGTLSEDTVRLFLVQLAGAMKALYTKGIVHRDLKPQNILLSHNYGKTLPAPSKITLKIA *F DFGFARFLNEGAMAATLCGSPMYMAPEVIMSLQYDSKADLWSLGTIVYQCLTGKAPFYAQTPNELKSYYEQNANLAPKIP *F SGVSPDLRDLLLCLLRRNSKDRISYESFFVHRFLQGKKAAVSPVDMPPLGGTPPAKAKSPLQQQLEQELKLVKLAEQQQK *F EREEQEAQEDENTVSVVANPAICATITNVGVLCDSENNSGSCSSHEDSDDFVLVPKNLPEDQRQGLAQVQAQPASGGQRP *F QQQQNQSSPPRPSSLPISEPKPVPAPARRQVARPGPLTVATLGGQQIPRSQPISVKQPRPDQRKSSVSSDINSISPPAVQ *F FAIGTPPTRMRSASGGSLSETPPPHAPSTWQVSPGHSQSPLRRSGNSSPVLPSAALTKLPTLGSPTMLVAPGSLGSIGSA *F GSGSENNNQHHMLGPRAFTLPELGATGGLHSLLDTGAGGGGEPHAFQAPELSEETLMDREHNETLSKLNFVLALTDCIQE *F VADSRCAPLSTFMVAGSQSAAQAASADAQQIPPHAPEHCKRAERLVLLVRGLQLLSSGMNLASQQLSNGQLKPSSNVKNA *F LLTMNAKYRSMLFESKRLNGSGLLQKANAFNITADKILYDYALDMCQAAALDELLKNTKNCFERYNTAHILLHSLVQKCN *F HPQDKMMLNKYRDAVEKRLSILQQHGYIYMTDENA *F >CG8866.AA *F MSLPRITDFEILEKLGAGSYATVYKARHKKQRTYHAIKYVEMSTLSQTSRENLITEIRLLRELKHKYIVTLQDFFWDDKN *F IYIVLEYCNAGNLSAFIRTKKALPESTCRYFLRQLAAAVQYMRANDVSHFDLKPQNLLLTRGANNVSLKVADFGFAQHLK *F LGEINQQLKGSPLYMAPEIVRKHQYDAKADLWSIGVILYECLFGKAPYSSRTIEELLLRIRKAEAITLPPNARISNECHD *F LLRRLLAHEPTARISFADFFAHPFLDLKTFPTEHTLQKAIDLVTQACAYDEKHNYKEAYYLYCSALQYFVPLITEETDAT *F KRLALRNRALSYTKRAEEIKNCIIEDEYRMLAERQRQAATAATANQEPSSATQVPAAQPSSSRVAEMLEPDSRYKQLYAL *F SNSSPSMKTGLEIGRKGELYLYERKLDAALESYTSALGILVPFVNNEPKGERRNLLLQQLEFWMKEAESIKSILSAKHLD *F DEEQKLSTRTSLRTITFQGWSKPPLIFFILFIVLNLIKIV *F >CG9746.AA *F MGNQLVGIAPSQIYAVEHYFSGQFGSEIAFSSNMGSTRFFKVAKAKTDEGQIVVKVFVKHDPTLPLEDHKERLEGIKKTL *F SLANAVNCLPFQRVELIDKAAYIMREYVKHSLYDRVSTRPFLTVLEKKWITFQILCALNQCHKQKICHGDIKLENILITS *F WNWILLSDFASFKPTYLPEDNPADYTYFFDTSRRRTCYIAPERFVKTLASDDDGGNGNMSVIHTDSIIRLAPSYAGNTLL *F PAMDIFSAGCALLELWTEGTAPFELSQLLAYRRGERDLVEKHLAGIENERLRNLLASMIDIHSMNRKSAEDYLDQERGQL *F FPEYFYSFLQSYLQMFSSTPIMSPDDKIQCLHKDIGHCIKVLTQAQDLTPEEEEKEEKKDPTLCARLPPEHDGLILIITV *F VTSCIRGLKQSNTKIAALELLQKLSKYTTSETILDRILPYILHLAQKSPAKVQVQAIETLTACLGMVKDIPRSDANVFPE *F YILPSITDLASETSAVIVRVAFARNIAALAKSAVYFLEETQRNAPNDMPTPRYEAELNALHDIVRQAVLSLLTDSQPVVK *F QTLMESGICDLCAFFGKEKANDVILSHIMTFLNDEDKNLRGAFYDNIAGVAGYVGWQASDILVPLLQQGFTDREEFVIAK *F AIRAVTILIELGHIKKPAVTEIVQETACYLCHPNLWVRHEICGMIATTARNLSAIDVQCKIMPAIGAFLKAPLIQVEKPH *F ILLDCVHPPVPRQIFDSVLRFQDIHHFIRALEARSRVRSQTRQGALPQYEEMGQTLRNLFRRLSSEGLTDLIEMQLLAMN *F PFLISMKHKALQDLDDTASGNGRIVVSRKQVACHEYPLADKASKMPLDNRSSEGPTLASPASDAAITPLGAGGLAGSPAS *F GAVVLGVPTATVSAATSLGEYTMPERNCWLERSSECRKDLESLTAKIKSRYNVLAISQRCSYDKLVTPYPPGWRMTGTLV *F AHLHEHSESVIKLASLRPHGSLFASGSIDGTVRLWDCSKLNGNQGVNKSRQVYSANTPIYALAACDSGQSLAVGGKDGSM *F LMMRIDRNSAKMTLQQALNQNDHKDGPVVDMHAYDQATQSVIVYATLYGGIVAWDTRMQHSAWRLQNELRHGVITTICAD *F PTGSWLATGTSGGKHICWDLRFRLPIAEIKHPADSWIRKVACHPTEPSYLISASQSNNEVSVWNIETGQRQTVLWASPVA *F ALSSASPSDPATTCGILSGVVDGSPFILTGSSDQRIRYWDITNPKNSSLKVPAANDNLADVAFNYGARLIDGSQVIEEQI *F ISMASTGDSQQLRSSTEENPRSGPDMPTASHHDAITDLLMCKDKGQIYIASASRNGVIKLWK *F >BcDNA:LD23371.AA *F MGKRLQLERPTTDRSARKRKRSAVKAAEKRQRLSGGSSSANGFEFHENDDEESCSSAGSAAGTEADPPTLLHTPQARSLL *F LTGASIASDHNNSSVMESPRPVYTLRPSVVNGTILRDVLSKAWRLGRPIGKGNFGEIFLASDDTVCPASSETAKYVVKIE *F PHSNGPLFVEIHCLINTSRNNDLSDAAEDAASLPAPQTHVLSRGPPSGIPSFIASGTHYFGDVRYRFLVLPRFDRDLHSL *F IKNSRVQQKSLLVLAVHIINVLENLHDKGYCHNDIKAQNLMVSKCKYLRRQVVPKGNGYEDHYEEKQQTTDSGNSSEQET *F NDDDYFLKSEKFALKKIVDIKQDEDEDDEDFDDGATSNSNNSNSLDVFHTPVNKKRSARNAIQFSGSNPVRACRREKRNS *F MYEEMVKSHYLRPTKRISYREEFNEDGYPKETAENSDESPESSDNESDEFIPPSSRRSVIKRGRSAQIATPKKTPVSTRA *F SRQEKVKKEPNGDQKLRSRGSKHLDNNPTEYKFLPTEEEHVFLIDFGLASKFQDRGVHRPFIMDQRRAHDGTLEFTSRDA *F HLGAHSRRSDLECLGYNLLYWSEGYLPWKDVAQQQQQEKVHRAKELFMTDVPEMLRQFYGKQVPKYLGEFLLQIGQLAYQ *F ERPNYERYRKIFKREYQRLGYDPCQMRLSSEEILRTCVSTKDVVDGSKCDIFELNNKAAVNVMRNSTLSTPFQEHSLTNR *F VSPKNLRSKSNKKTTKKKFSWAEVLSQDPDQIARERAVKEFEREETICPLESRLPRRYEGKPTYAILDMEQRRREKGLVV *F QEHIEEEEEDADEDDEEENQEAMDIDQEEDGEAADSAEGEDESDRSMEGSDCSDHSQKRARGRPKGTSRKQTTSRQAQPH *F QNQPPVKVHRGVGRPGKNSGVVKLAAGAVSKNRTTPLSAVASNKRGCATRKENSTLASATGEGERKLKSGRTRRALYKTE *F PKHGEHDAENNSSLLVVQNLYGEYDDENNYGKGRSVHSSRHCRK *F >BcDNA:LD09009.AA *F MPRVAKPKAAAPAKKVVSAKKAKSKLYKMPEKVKEGTVFTDLAKGQWRIGPSIGVGGFGEIYAACKVGEKNYDAVVKCEP *F HGNGPLFVEMHFYLRNAKLEDIKQFMQKHGLKSLGMPYILANGSVEVNGEKHRFIVMPRYGSDLTKFLEQNGKRLPEGTV *F YRLAIQMLDVYQYMHSNGYVHADLKAANILLGLEKGGAAQAYLVDFGLASHFVTGDFKPDPKKMHNGTIEYTSRDAHLGV *F PTRRADLEILGYNLIEWLGAELPWVTQKLLAVPPKVQKAKEAFMDNIGESLKTLFPKGVPPPIGDFMKYVSKLTHNQEPD *F YDKCRSWFSSALKQLKIPNNGDLDFKMKPQTSSNNNLSPPGTSKAATARKAKKIDSPVLNSSLDEKISASEDDEEEEEKS *F HRKKTAKKVTPSARNAKVSPLKRVADSSPPSQKRVKTEPKSTPRERATPKASPKPRSTPKASPKPQTPTAARLRTPNAKI *F NFSPSISLRGRPGGKTVINDDLTPQPRSKKTYEFNFELDVSMDANVIVNVKRKKKADQDKATAVDSRTPSSRSALASSSK *F EEASPVTRVNLRKVNGHGDSSTPGRSPRTPAVTVRKYQG # *U FBrf0151621 *a Thorpe *b C. *t 2002.11.6 *T personal communication to FlyBase *u *F From owner-dros@hgmp.mrc.ac.uk Wed Nov 06 22:29:35 2002 *F Envelope-to: ma11@gen.cam.ac.uk *F Delivery-date: Wed, 6 Nov 2002 22:29:35 +0000 *F To: bionet-drosophila@net.bio.net *F From: cthorpe@UDel.Edu (Colin Thorpe) *F Newsgroups: bionet.drosophila *F Subject: ECM Disulfide formation: sulfhydryl oxidases *F Organization: University of Delaware *F MIME-Version: 1.0 *F Content-Transfer-Encoding: 7bit *F X-Received: from localhost (localhost [127.0.0.1]) *F by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9D29C7D12E *F for ; Wed, 6 Nov 2002 16:45:15 +0000 (GMT) *F X-Received: from copland.udel.edu (copland.udel.edu [128.175.13.92]) *F by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id A7F547D170 *F for ; Wed, 6 Nov 2002 16:45:14 +0000 (GMT) *F X-Received: from udel.edu ([128.175.54.224]) *F by copland.udel.edu (8.12.6/8.12.6) with ESMTP id gA6Gj7ch014602; *F Wed, 6 Nov 2002 11:45:13 -0500 (EST) *F X-Mailer: Mozilla 4.7 [en]C-CCK-MCD (Win98; I) *F X-Accept-Language: en *F X-To: dros@net.bio.net, cthorpe@UDel.Edu *F X-DCC-UDEL-Metrics: copland.udel.edu 101; Body=2 Fuz1=2 Fuz2=2 *F Date: Wed, 6 Nov 2002 22:29:35 +0000 (GMT) *F X-Razor-id: 7a28006aea079617870713710a436ad2a3952421 *F Sender: owner-dros@hgmp.mrc.ac.uk *F *F I wish to advertise a newly-discovered family of enzymes involved in *F disulfide bond formation in secreted proteins and peptides. My hope is *F that someone in the Drosophila community might be interested in *F following them up. *F *F These sulfhydryl oxidases are found in all multicellular organisms. *F They are not found in yeast. The human counterparts have been called *F 'bone-derived growth factor', 'cell inhibitory factor' and 'quiescin'. *F *F Drosophila has 3 or 4 such sulfhydryl oxidases genes (CG4670-PA; *F CG17843; CG6690-PA; CG31413-PA). Their roles are unknown. *F *F Circumstantial (non-knock-out) evidence in mammals suggests involvement *F in ECM formation and differentiation, in spermatogenesis, and in *F secretion of peptides and proteins. *F *F For a recent review on these enzymes, and some nice pictures of cellular *F distribution in human tissues, see: *F *F 'Sulfhydryl oxidases: emerging catalysts of protein disulfide bond *F formation in eukaryotes' (2002) Thorpe et al. Archives of Biochemistry *F and Biophysics 405, 1-12. *F *F Thank you. *F *F Colin Thorpe *F *F Professor of Biochemistry *F Department of Chemistry and Biochemistry *F University of Delaware *F Newark, Delaware 19716 *F Phone: (302) 831-2689 (FAX -6335) *F http://www.udel.edu/chem/thorpe/ # *U FBrf0151622 *a Goodwin *b S. *t 2002.8.27 *T personal communication to FlyBase *u *F Date: Tue, 20 Aug 2002 11:01:24 \+0100 *F To: Robert Levis *F From: Markus Noll *F Subject: Re: FlyBase Query (cy1670) *F Cc: efrei@molbio.unizh.ch, michaeld@molbio.unizh.ch *F .. *F ..(Hochman changed) the nomenclature of the alleles named in his first *F paper with Gloor and Green (1964) in a subsequent paper, which he *F authored alone (1971). We have carefully read all the literature *F available, including short communications in DIS, have actually tested *F all the alleles still available from FlyBase, and tried to give this *F information in the paper (text and legend to Fig. 3). However, it *F might still not be sufficiently clear, for which i apologize. I will *F ask Erich Frei, who did most of this work together with Michael Daube, *F to help you. *F With best regards, *F Markus *F Dr. Markus Noll *F Institute for Molecular Biology *F University of Zürich *F Winterthurerstrasse 190 *F CH-8057 Zürich *F Switzerland *F e-mail: noll@molbio.unizh.ch *F phone (office): \+41-1-635-3130 *F fax (personal): \+41-1-635-6829 *F home page: http://www.unizh.ch/imb/noll *F >Dear Dr Noll, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Kronhamn et al., 2002, Development 129(4): 1015--1026 *F > *F >I have some questions, as I have to admit, I am somewhat confused about *F >the relationship between the genes you mention in the toy region, and *F >the genes FlyBase has with similar names or synonyms. *F > *F >You describe an allele l(4)8 whose headless phenotype you discuss. You *F >then state that it is allelic to toy. You cite the Hochman 1964 paper, *F >for l(4)8, and I can find in that paper a complementation group that *F >contains mutations 8 10 and 19 (Fig1). *F > *F >We have l(4)8 as a synonym for l(4)102CDg (specifically l(4)102CDg1) *F >We have l(4)10 as a synonym for l(4)102CDh AND l(4)102CDg *F >(l(4)102CDg2 aka l(4)8a) *F >We have l(4)19 as a synonym for l(4)102EFf and l(4)102CDg *F >(l(4)102CDg3 aka l(4)8b) *F > *F >This would suggest that the mutations 8 10 and 19 and thus the l(4)8 *F >'hdl' are alleles of l(4)102CDg, however you name (4)102CDg as a *F >separate gene in Fig3. Which means that the gene FlyBase currently *F >calls l(4)102CDg is probably not the gene you call l(4)102CDg. *F > *F >You mention that there has been confusion about nomenclature in this *F >region, and I have had a look at the Hochman papers involved, but it *F >hasn't really cleared things up for me. *F > *F >Is the toy allele 'l(4)8=hdl' allelic to any of the FlyBase genes? *F >l(4)102CDh, or l(4)102CDg? Or is the allele l(4)8 you used not *F >described in FlyBase (possibly merged with another l(4)102CDg by *F >accident? when the symbol l(4)8 was reused) *F > *F >You mention that the allele you call l(4)102CDg3 was previously called *F >l(4)19, which means that FlyBase probably assumed that it was the same *F >as l(4)102CDg3 above. In which paper was this allele called l(4)19? *F > *F >Have we merged two genes by accident? l(4)102CDg with three alleles *F >called 8 10 and 19 in the Hochman 64 paper A different gene with the *F >single allele called by you 102CDg3? *F > *F >To summarise: *F > *F >You mention these three genes, *F >102CDh (2 alleles) *F >102CDg (1 allele) *F >toy(l(4)8) *F > *F >FlyBase has currently, *F >l(4)102CDh (3 alleles) *F >l(4)102CDg (3 alleles) *F > *F >Can you help me establish which genes are which? Any new information *F >you give us will be curated as a personal communication, if thats OK *F >with you. *F > *F >Thanks *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: c.yamada@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph: 01223-333963 *F >United Kingdom. FAX: 01223-333992 *F >---------------------------------------------------------------------- *F Dear Chihiro, *F .. *F Erich and I went once again through our notes and summarized our *F findings and conclusions in the attachment that should answer all of *F your questions and help you eliminate ambiguities in the annotations of *F FlyBase. *F With best regards, also from Erich, *F Markus *F \-- *F Here is what caused the confusion. In 1964, Hochman, Gloor, and Green *F published a paper (Genetica 35, 109-126) describing 54 lethal mutations on the *F 4th chromosome, 36 of which were induced by X-ray by Gloor and Green, while *F the remaining 18 were isolated as spontaneous mutations by Hochman, largely *F from natural populations. These 54 mutations were grouped into *F complementation groups, including one or several alleles. The X-ray induced *F mutations were given numbers, while the spontaneous mutations were designated *F by a two or three-letter code followed by a number. The X-ray induced and *F spontaneous mutations were grouped into complementation groups. For example, *F the following X-ray induced mutations were found in the same complementation *F groups: *F 1, 27, 28, 32 *F 2, 21, 23, 29, 34, 37, 38 *F 4, 18, 20 *F 5 *F 6, 15, 36 *F 7 *F 8, 10, 19 *F 11, 12, 24 *F 13 *F and so on! *F The confusion started when Hochman published the next paper in 1971 (Genetics *F 67, 235-252), in which he decided to 'replace the nomenclature used earlier *F with one in which all lethals are symbolized by an italicized number, e.g., *F l(4)1, and all alleles found subsequently are given the same number and a *F letter superscript. Thus the lethals allelic to 1 described earlier (27, 28, *F and 32) become 1a, 1b, and 1c, respectively. This practice serves to release *F integers such as 27, 28, and 32 in naming newly detected loci and it also *F provides a means of computing rapidly the number of allelic mutations that *F have occurred at each locus.' *F In other words, the complementation groups above became: *F l(4)1, l(4)1a, l(4)1b, l(4)1c *F l(4)2, l(4)2a, l(4)2b, l(4)2c, l(4)2d, l(4)2e, l(4)2f *F and so on. *F Specifically, the alleles of the earlier complementation group, 8, 10, and 19, *F were renamed as l(4)8, l(4)8a, and l(4)8b. As a consequence, 10 and 19 *F were 'released' and reused to designate new alleles of different loci! *F Therefore, what later appeared as l(4)19 is not allelic to l(4)8, as the *F uninformed reader might suspect, but rather represents a new locus! *F In contrast, the mutant allele l(4)8 that Åsa Rasmuson-Lestander discovered *F among the Umeå stocks to have a headless phenotype (Kronhamn et al., *F Development 129, 1015-1026, 2002) never changed its original name and was *F still listed under this name in the Umeå stock list. We have shown that this *F mutation is a deficiency of the toy gene that deletes the entire coding region *F of the four last exons, exons 6-9. Because of its headless phenotype, we *F named the l(4)8 allele toyhdl. *F Apparently, FlyBase was also confused by the different nomenclatures of *F Hochman. Therefore, all lethals on the 4th chromosome were given new names *F according to their suspected cytological position. For example, l(4)8 was *F also called l(4)102CDg1, while l(4)10 was called l(4)102CDh as well as *F l(4)102CDg2 (aka l(4)8a). The trouble is that there are two different *F l(4)10 mutations: one is the old mutation described in 1964, which in 1971 was *F renamed l(4)8a by Hochman and later l(4)102CDg2 by FlyBase; the other *F l(4)10 mutation was first published in 1971 and, accordingly, is not allelic *F to l(4)8. This mutation was given the name l(4)102CDh by FlyBase. *F The same problem arises with the l(4)19 name. The trouble is that there are *F two different l(4)19 mutations: one is the old mutation described in 1964, *F which in 1971 was renamed l(4)8b by Hochman and presumably later *F l(4)102CDg3 by FlyBase; the other l(4)19 mutation was first published in *F 1971 and, accordingly, is not allelic to l(4)8. This mutation was given the *F name l(4)102EFf by FlyBase. From the Bloomington as well as the Umeå Stock *F Centers we have obtained the l(4)102CDg3 stock (# 1089 from Bloomington and *F \# 73560 from Umeå). In both cases, this stock complements with l(4)8 and *F maps to the region closest to the telomere. Therefore, we concluded that it *F was the l(4)19 mutation described by Hochman in 1971 and not that described in *F 1964 to be allelic to l(4)8 and renamed l(4)8b in 1971 by Hochman! In our *F paper, we used the name given by FlyBase and/or the Stock Center, *F l(4)102CDg3. The mutation l(4)102EFf of FlyBase does not exist as stock in *F the Stock Center. However, what the Stock Center carries as l(4)102CDg3 *F stock is more appropriately called l(4)102EFf, as we have shown, and *F presumably corresponds to the l(4)19 mutation described and mapped to the *F telomere by Hochman in 1971. *F In summary, we know that (i) the 102CDh1 and 102CDh2 mutations (and *F alleles of the Stock Center) are allelic and located between ey and toy as *F described (Kronhamn et al., 2002); (ii) the l(4)102CDg1 allele is identical *F to l(4)8, which never changed its name, and a toy allele with a headless *F phenotype, which is why we called it toyhdl (Kronhamn et al., 2002); (iii) *F the l(4)102CDg3 allele (and stock of the Stock Center) maps to the most *F distal portion of the right arm of the 4th chromosome and hence is presumably *F identical to the l(4)19 mutation mapped to the same region by Hochman in his *F 1971 paper. Therefore, this stock should be called more appropriately *F l(4)102EFf, a name already carried by FlyBase for the most distal lethal *F mutation on the right arm of the 4th chromosome. # *U FBrf0151626 *a Levis *b R. *t 2002.8.13 *T personal communication to FlyBase *u *F Date: Tue, 13 Aug 2002 16:40:43 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: Merge Tapdelta and BcDNA:GM12291? *F It appears to me that the FlyBase genes Tap-delta *F (Translocon-associated protein-delta) and BcDNA:GM12291 are probably *F synonyms. FlyBase lists one allele of the Tap-delta gene, which is *F Tap-deltak17005. The P-element insertion in this allele is *F inserted at the 5' end of the gene named BcDNA:GM12291. The gene *F report for BcDNA:GM12291 states: 'It encodes a product which is a *F component of the translocon.' There are no mutant alleles listed for *F BcDNA:GM12291. Neither of the gene reports for the two genes lists *F the other as a synonym. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0151627 *a Ashburner *b M. *t 2002.8.15 *T personal communication to FlyBase *u *F From ma11@gen.cam.ac.uk Thu Aug 15 11:04:03 2002 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Thu, 15 Aug 2002 11:04:03 \+0100 *F To: gm119@gen.cam.ac.uk *F Subject: te updates *F Cc: ma11@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Michael Ashburner (Genetics) *F Date: Thu, 15 Aug 2002 11:04:06 \+0100 *F Content-Length: 7927 *F Transposable element update from M.A. *F ==================================== *F 1. Name changes: *F Pilgrim (FBgn0045970) to Tabor *F Mercurio (FBgn0026410) to Tc1 *F Tinker (FBgn0043969) to diver *F Waldo-A (FBgn0042682) to Rt1b *F You (FBgn0043055) to Ivk *F pilger (FBgn0041728) to Rt1a *F 2. Merge: *F Strider (FBgn0062256) should be merged with Juan, as Juan. *F cruiser (FBgn0044355) should be merged with Quasimodo, as Quasimodo. *F 3. New references to be added: *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 1997- *F \*u A reference collection of Drosophila repetitive sequences. *F \*w http://www.girinst.org/ *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u G5_DM, an ancient family of non-LTR retrotransposons from the Jockey clad. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 2 *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u ROOA, an ancient family of endogenous retrovirus ancestral to the modern *F ROO family. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 9 *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u G4_DM, an ancient family of non-LTR retrotransposons from the Jockey clad. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 1 *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u JOCKEY2, an ancient family of non-LTR retrotransposons from the Jockey clad. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 5 *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u G6_DM, an ancient family of non-LTR retrotransposons from the Jockey clad. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 3 *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u POGON1, a 'bona fide' family of nonautonomous DNA transposons. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 7 *F \# *F \*a Kapitonov *F \*b V.V. *F \*c J. *F \*d Jurka *F \*t 2002 *F \*u Looper1_DM, a family of Looper/PiggyBac DNA transposons in D. melanogaster. *F \*w Repbase Rpts *F \*y 2 *F \*Y 3 *F \*z 6 *F \# *F ==== *F new starw *F \*w Repbase Rpts *F \*V 1- *F \*t 2001- *F \*u Repbase Reports *F \*w http://www.girinst.org/Repbase_Reports.html *F \# *F ====== *F 4. Adds *F \*a pogo *F \+ *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 7 *F \*E Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 7 *F \*i POGON1 *F \# *F \*a Dm88 *F \+ *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*i copia2 *F \# *F \*a Rt1b *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Rt1a *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Ivk *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Tc1 *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a F-element *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Helena *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a roo *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a HMS-Beagle *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Tirant *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Quasimodo *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a Tabor *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a 1360 *F \+ *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*i protop *F \*i protop_b *F \# *F \*a diver *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F \*a opus *F \+ *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*i nomad *F \# *F \*a blood *F \+ *F \*x Kapitonov and Jurka, 1997- *F \# *F 5. New records: *F \*a gtwin *F \*x Kapitonov and Jurka, 1997- *F \*x FBrf0138423 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \*i gtwin *F \*E FBrf0138423 *F \*i hamilton *F \# *F \*a gypsy2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a accord *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a gypsy3 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a gypsy4 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a gypsy5 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a gypsy6 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a Doc2-element *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \# *F \*a Doc3-element *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \# *F \*a invader1 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a invader2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a invader3 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a invader4 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a invader5 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a Stalker2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a Stalker3 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a G2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i G2_DM *F \# *F \*a G3 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i G3_DM *F \# *F \*a G4 *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 1 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i G4_DM *F \# *F \*a G5 *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 2 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i G5_DM *F \# *F \*a Cr1a *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i DMCR1A *F \# *F \*a Rt1c *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i DMRT1C *F \# *F \*a baggins *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i baggins1 *F \# *F \*a mariner2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \# *F \*a transib1 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \# *F \*a transib2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \# *F \*a transib3 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \# *F \*a transib4 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \# *F \*a diver2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \# *F \*a S2 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \*i S2_DM *F \# *F \*a rooA *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 9 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \*i ROOA *F \# *F \*a jockey2 *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 5 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \# *F \*a G6 *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 3 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LINE *F \*i G6_DM *F \# *F \*a looper1 *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 6 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \*i LOOPER1_DM *F \# *F \*a Tom1 *F \*x Kapitonov and Jurka, 1997- *F \*x Kapitonov and Jurka, 2002, Repbase Rpts 2(3): 9 *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: LTR *F \*i DMTOM1 *F \# *F 6. merge Ars4 >> INE-1 *F \*a INE-1 *F \+ *F \*i Ars4 *F \*x Kapitonov and Jurka, 1997- *F \*E Kapitonov and Jurka, 1997- *F \*t transposable_element *F \*l element type: IR *F \*i narep1 *F \*i ARS312 *F \# # *U FBrf0151628 *a Kaminker *b J. *t 2002.1.16 *T personal communication to FlyBase *u *F Date: Wed, 16 Jan 2002 15:04:52 \-0800 *F From: Josh Kaminker *F To: ma11@gen.cam.ac.uk *F Subject: FROGGER sequence *F attached is FROGGER *F Date: Wed, 16 Jan 2002 15:05:14 \-0800 *F From: Josh Kaminker *F To: ma11@gen.cam.ac.uk *F Subject: ROVER SEQUENCE *F attached is rover seq *F >FROGGER|LTR 1-203|LTR 2281-2483| POLYMERASE 240-1859 (broken) *F TGGTTGCATAAGAGTGCCGCCGCGGTAAAATGATTGAGACAATGAGATGT *F GATTAGGGTGAATGCTTTATTAGAACTGAACTGTGTACATAACTTCGTAT *F ACATGCTTCACTACTTTGAGTGGCAAATGAGAGCAAGAGAAGCTTGCTCC *F CTGAGAGAGTAATAAAGTTGCCAGACAGATGATACGCAATCTTATATTTC *F CAACACTTTTGCTTAAGATCAACATCATCTGTTTTGCAATTACAAATTTA *F AACAAATCCAAGTGATTTCAAAAAGCCACAATGCTTTTGCTTTGGTTAGT *F ATATCTGCGACATTATTCTGAGTGTTAACATATTCAACAATTATTTCATT *F TTTGTTAACTTTGTCCCTTATAAAATGATATCTGACATCAATGTGTTTTG *F TACGCTTGTGATTAATTGGATTCTTGGCAATACTCGAGGCGCTCAGATTG *F TCGCATAGTAGTTTCAATGGCCCCTTGTCGAACCATCCCATCTCTTGTAA *F GAGTCTTCTTAAAAACACTGCTTCTTTTGCTGCTGTTGATAAGGCGATGT *F ATTCAGCCTCGGTGCTGCTTTGGGCGATGACGCTCTGCTTTGATGAGCCC *F CATGAAAATGCACTTCCAGCCATAAGGAATGCATATCCTGAATACGACTT *F TCGGTCGACTTTGTCGTTTGCCCAATCTGCGTCTGCGAATTCCTTGACCA *F GCTCGCCGGTCTTCATGTAGATAATGCTCAGGTTGATTGTCCCACACAAA *F TATCGTAGCACATGCTTTGCTGCTGCTTCGTCTTCGTGGTGAGGATCTGT *F ATTTCTCTGTGATAACTTGCTGACAGAGTGCAATATGTCTGGTCGACTTA *F CTACTGCTAAATACATAAGCGAACCAATTAGTGATTGAAAATGGGTTAAA *F TTTACCTTTGCACATTTCTCATTTGTGCAACCAGATTCATAACCAGGGTC *F AAATGGGGTTCCCACTAGTCGACAGCTTTGCATGCCATACCGTCTTAGTA *F GATTTTCAATGTAGCGCTTTTGACATAGCTTTATAGGACCTGTTTGTCCC *F TTTCGGCTTATTTCCATCCCAAGGAAATATCTCAGTTGGCCGCCATCCTT *F CATATCAAACTTTGTTGCTATGCTCCCTTTGATGTCTTCTATGTCGCTTT *F CTTTGGGAGATATGATGATCAAATCGTCAACGTAGACAGCTATATAGCTA *F AAACCGTTTTGTTGCTTCTTGTAGTATAAACATGCCTCGTTCTTGCTTCT *F TTTAAAGCCCATATTTTGAAGAACTTCATTTAAGGCATCGTTCCACAGTC *F GTTCAGACTGCTTAAGCCCATAGATTGCTTTTTGCAACTTAAGGACTTTT *F CCAGGATTTTCCTTGTCAACGTAATTTTTTGGTTGTTTCAAATAAACATC *F CTCCTCAAGGTCGCTGTTTAGGTATGCGTTTGATATATCAATTTGATGCA *F TGTGAAGGTCTTTTTCTGCAGCAACTGCAAGCAGCATCCTGATGGTCTCG *F TACCTGATTACCGGGGAATATGTTTCCCAGTAGTTCACTCCAAATTGTTG *F TCCACAACCTTTGGCAACAAGTCGTGATTTGAATCTCTCTATTTCGCCAT *F CCTTGTCTTTCTTGATGCGAAATACCCATTTTGATCCAATTGCTTTTGGG *F CAGGTCTACCAGTGACCATGTATTGTTGGCTATTAACGCTTCATACTTCG *F CTCGCATTGAATCCTGCCAATTTACAATTGGCCCTTGAGTGCTTGCTCCA *F ACGACTGCGGTGTTTCTTCATCTTCCTCGTAATTCAAATATTGAAGAGTT *F TGATACACCTTTTTCGGCCTTCCTGGTTGGCCAGTTCGTATGAACTTTGG *F TCGGCCTGGCCTTCGCTGTTGTGCCATTAGGGTGTCATCTGCAGATTGGT *F CATCAGAGTCGTCATTGGCACTGACAAACGTCCTGCCCTCATCGCTGTCG *F TAAACGGCTTCTTCATCGTCAGTACTGCTGGCTTCAGCTAGCAAGTGACG *F TTTCAGCCCATAAAAATTCATCCAAACCAGCATGTATTAAAAGACTTTTG *F GCCATTTCCACAATTGTGCGGTTTGCACGTTCAGCAATTCCGTGTTGCTG *F AGGGGTATACGAGACACTTAACTGTCTCTTTATCCCGCATTGGCTTAAAC *F AAAGATAGACGCGAGTTCTTTTCTCTTAACTTTGTTTTATTTTTGGTTAC *F TTCACTTTGATCACTTCACGAATACTTTGTAAACGAATCAATACATTTCT *F TTACGCGTAGGGCCTGGGCCCATAACCAAATGTTTGCATAAGAGTGCCGC *F CGCGGTAAAATGATTGAGACAATGAGATGTGATTAGGGTGAATGCTTTAA *F TAGAACTGAACTGTGTACATAACTTCGTATACATGCTTCACTACTTTGAG *F TGGCAAATGAGAGCAAGAGAAGCTTGCTCTCTGAGAGAGTAATAAAGTTG *F CCAAACAGATGATATGCAATCTTATATTTCCAA *F >ROVER|LTR 1-367|LTR 6952-7318|Gag 1282-2535|Pol 2595-5144|Env 5823-6575 *F AGTAACATAATATGCTTCTCATATTACGTTTACATACTTACACTAATTGT *F ACATACAATCTTGCACATGCATAAACACATGAAACCAGTTTACATTTTTA *F CTTACACTTAAGCGCATAATTTGTTGTGCATCCATACCGTTATTTTTCCG *F TTCTTTTTTGTACACATATACTGATTAGACATTCCCGTTTCTCGCGACTC *F ACTTCGAGCCGATCAAAAACTCTGTACAGTCAGTCTTAAGCCGACAACGA *F AGAAATAAAGATCTAAACTAAAAAATACCTCGTGTTGATTCTGAAACTTC *F TTTAAAGGCGTTGATCTTAGTCAAACGACGGATCATTTGTTCGACTCGAA *F TAGTAAAATACGTAAGTGGCGCAGTCGGTAGGATAATACATTGTTGATGC *F GATAGTAACCCTATTCAATTCTTCAATTATTATCCACAAAAGAACTAACG *F CAGTCGCGTCGTGCTAAGTGGAAAGTGTTAACCAATGGTACTCAATCCCA *F TAAAGTGACCACGAAGTGAAATAACTTAAAATTACAAAAATTTTAAGAAG *F ACGCCTTTGAATTCGCTGAGTAGTAAATCCACAAAAGGTTACTCAAACCA *F AAGCGAAACAGCTAAGAGCGCAACACAACAAACTTAAATTAAAAACTTAC *F AAATAAGTGAATAACAGAAATATTACGAACAAATAAGTGCGAAAATTCAA *F CAAAACCTAAGCTAAAGAACTATAACTAAGTGAATAACAAACATTATAAG *F TGAATTAAATTAATAAGTGAACAACACAACAAACTTAAATTAAAAACTTA *F CAAATAAGTGAATAACAGAAATATTACGAACAAATAAGTGCGAAAATTCA *F ACAAAACCTAAGCTAAAGAACTATAACTAAGTGAATAACAAACATTATAA *F GTGAATTAAATTAATAAGTGAACAACACAACAAACTTAAATTAAAAACTT *F ACAAATAAGTGAATAACAGAAATATTACGAACAAATAAGTGCGAAAATTC *F AACAAAACCTAAGCTAAAGAACTATAACTAAGTGAATAACAAACATTATA *F AGTGAATTAAATTAATAAATGAACAAACACAAACACAAAGCTCAGACCTG *F GAATACAAACTAATAGTGGAAATCAAGAACCCATAGTGAAAACCAAAACC *F CTATCCAAAACATAAACCTACAGTAAAAACTTGAACTTTATCGAAAAACG *F AAACCTATCCGAAACTGCAAACCTTACCCAGAACGTGAACCTTACCTAAA *F ACATTAAATAACAATAAATACAACAACAACAATGGCAACAGCAAGCCCTA *F TAATACTATCCGACTCAAATATGAGTCAGGTCGAGAGACAGATAAATGCT *F GTCGAAATCTTCAATGGAGACCCGAATACTCTACATACCTTTATAACTCG *F CATCGACTTCATTCTGGCCTTATACCAAACTACTGACGAAAGGCAGAAGT *F TGATAATTTATGGACACATCGAACGCAATATCAGCGGCGACGTCATCCGT *F ACTCTCGGGACCAACAACTTTACCAGCTGGATTGAACTAAGGACCAGATT *F GATCCTATATTACAAACCCCAGGCACCGAGTCATCAACTTTTGGAGGATT *F TTCGGAACATTCAATACAAAGGCAACATCAGGCAGTTTCTGGAGGAAGCC *F GAGAAAAGACGACAAGTTTTAATGAGTAAGTTAGAACTAGAAAATAACTC *F AGCTGAAACCGCCTTATTTACCAGACTTATTCAAGATAGCATAGAAAACT *F TGATCCTAAAACTCCCTAACCATATTCATCTAAGAATAGTTAACTGCCAA *F ATCCCCGATTTAAGATCCCTTATTAATATCTTACAAGAAAAGGGTCTATA *F TGAACCTCAAACATTAAACAAAGATAACTTTAAGCCAGCTCCTAATTCTA *F ATAGGACGCCAAATAACTTACCAAATAGACAAGTTAATGGCCAAAATAAA *F CCTATAACACCTTTTCAACCGTACCATAACAACGTCTTTCAACCCTATTA *F TCCACCATACCCATACGCACCAATCCATACACCCAGGCCCAATCAAACCC *F CTTTTCCCCAATATCAACCAAGACCAACCTACCCACAACCTAACATACCC *F AGACCGAATCCTGTCTTTAATCGACAGAATATCTTCGACCAAAATCGTTT *F CGGACCTAATCAAACTTACACACCGAATAACTTCCCTCCAACTGGGAACA *F CACAAACCAACAACCCGGTAAAAAGACAACGACCATCAGACAGCGGACAG *F ACTAAAATGAGCATAGAAGAACTAAGATACCAAGAAATGGTATCAAACCA *F ACCCGAATACCCTTACCATTATTACTATCCATACTACCCAGATAACTTCC *F AACCACAACCATATCCTTATCAAATGAGTTATATCCCAGACCCAACTCAA *F ATCCCTTTCGAACAAGATCATGACACTTCGGACAAAACACAACAAGAACA *F GACAGCACCAAACGATGACGAATCAGACAATACCAAAGAAGCAGAAAATT *F TTCGGCTAGTTGCCCCGGATCAAACCAATATATAATTATTAAACATAATG *F ACATAGACTTAAAGTGCCTAATTGACACTGGATCCACCATAAACATGCTT *F ACTTGGAACATTTTCGAAACACCTATACAACAAACCAACCACCTAATTCA *F TACAAGTAATGGCTCGGTAACTATTAACGAGACCACCACTATACCACCCA *F ATAACTACTTTCCTATTGCTCAGGAGTTTCTAATTCACAAATTCTCAGAT *F CATTATGATATGCTGATTGGACGCAAATTGTTGTCTAAAGCTAAAGCCAT *F AATAGATTATCAAAAGAAAACTGCCACCCTCTTCAACAAAGTCTATAAAA *F TAAAAGACACTGAAAAACTTACAGACCAAAATCATGCTCAAGTAGACTCT *F TCGTTCCCACAAGACCCTACCTTTTTAGAAACCTCCACACTTCAGGAGAA *F TCTGTTCCGACTAAACCATTTAAATGCAGAAGAAAAATACAAATTAACAA *F ACTTACTGACAAGATTTAAAGACATTCAGTTTCACGAGGGCGACAAACTT *F AGCTTCACAAACCAAGAAAAACATACAATTAATACCACACATAATATTCC *F AGTTTACTCTAAAATGTATAGCTTCCAACAATCATACGAACAAGAAGTAG *F AAAGACAAATTCAAGAAATGTTAGAACAAGGCATCATTCGAGAAAGCAGC *F TCACCGTACTGCAGCCCCATCTGGGTAGTACCAAAAAAATTAGATGCTTC *F CGGACAGCAGAAACTTCGAATAGTCATAGACTACAGGAAGCTGAATGAAA *F TAACAATAAATGACCGATACCCTATGCCAAATATAGACGAAATACTAGGA *F AAATTAGGGAGGTCTAATTACTTTACCACCATAGACCTGGCAAAAGGCTT *F CCATCAAATAGAGATGGATTCAGAATCAATAGCCAAAACGGCTTTCTCTA *F CTAAATACGGACATTACGAATATACTAGAATGCCATTTGGGCTTAAAAAC *F GCCCCAGCTACCTTCCAACGCTGCATGAATAACCTACTTCGTCCGCTTTT *F AAATAAAAATTGTTTAGTATATCTAGACGATATCATTGTCTTTTCTACCT *F CTCTAGAGGAACACCTTCAATCCCTTGAAGCAGTCTTCGAGAAATTATCT *F CAAGCCAACCTTAAACTACAGCTAGATAAATGTGAATTCCTAAGACAAGA *F AACTACCTTTCTAGGACACGTTATCACTAAAGACGGAATTAAACCAAATC *F CCGAAAAAATCAAAGCTATACAAGATTACCCACTTCCATCTAAACCTAAA *F GAAATTAAAGCATTCCTTGGAATCACAGGATACTACAGGAAATTCATACC *F CAACTTCTCTGACATAGCGAAACCACTGACTAAATGTCTCAAAAAGGGAG *F TAAAAATAGATACAAAACATAAAGAATATATAGAAGCATTTCAAAAATTA *F AAATTACTTATTTCCGAAGACCCCATATTAAAAATACCAAATTTTGAAAG *F AAAATTTGTATTAACAACTGACGCAAGCAATGTAGCATTAGGTGCTGTTC *F TATCTCAAGATGGACATCCCATTAGCTACATTAGCAGGACCCTAAACGAA *F CATGAAGTTAATTACAGTGCTATAGAAAAAGAACTACTAGCAATAGTTTG *F GGCCACAAAGACCTTTAGACACTACCTGTTAGGACGACATTGCGAAATAG *F CTTCTGACCATCAACCATTGTGCTGGCTACACAAATTAAAAGAACCTAAC *F TCAAAGCTGACTAGGTGGAAGATTAGATTATCCGAGTACGACTTCGATAT *F AAAATATATCAAAGGAAAGGAAAACCATGTCGCAGACGCACTGTCTAGAA *F TCAAAATAGAACAAGCATTTTTCGGAGAATCTACGCAACATAGCGCAGAA *F GAAGATAATAGCGACTTAATAGGGTTGTCCGAAAAAGCCATTAATTACCA *F TAAAAGACAAATTATTTTCTCAAAAGGTCCCAATAGTAGTATAGAACACG *F AAACTTATTTCAAAAAACAAATCATACACATTTCCTACAAAGAAATGACC *F CTAGAAGCAGCCAAACAATATCTACTCAATCACTTTTGCTCAAAGAATAG *F CGCTCTTTACATCGAAAGCGATGCAGACTTCGAAATGATCCAAAATGCAT *F ACACAACAACAATGAATCCGAAATGTACAAAAATTTTCCGAAGCCTCGTT *F TTATTAAAAAACATCCCTACTTATGCAAGTTTTAAGGAACTAATTCTAAA *F CACCCACGAAAAGCTCTTACACCCCGGAATTCAAAAAACTGTAAAACTCT *F TTAGTGAGAATTATTACTTCCCCAATAGCCAACTCCTTATCCAGAATATA *F ATCAACGAATGTCAAGTCTGCAACCTAGCCAAAACAGAACACCGTAACAC *F GGAAATGCCAATGAAAATTACCCCTAAACCCGAACATTGTCGTGATAAGT *F TCGTAATAGACATCTATTCTTCCGAAGGAAACCATTATCTGAGCTGTATC *F GATATTTACTCTAAATTCGCCACCCTTGAACAGATTAAGACAAAAGACTG *F GGTAGAATGCAAAAATGCCTTAATGCGCATATTTAATCAATTAGGGAAAC *F CTAAACTTCTCAAAGCAGATCGAGATGGTGATTCTCCAGCCTAGCCCTCA *F AACGTTGGCTCGAAACCGAAGAAGTAGAGTTACAACTTAATACTACAAAA *F ACAGGTGCGCTGACATAGAGAGACTCCACAAAACTATAAACGAAAAAATC *F CGCATCATAAAAAACTCTGACGACAACGAGACCAAATTAAGTAAAATAGA *F AACAATCCTTTACATTTACAACCACAAGACCAAACACGATACTACCGGAC *F AAACCCCTGCTCACATATTCATTTACGCCGGACAACCTAACTTAGATACA *F CAAAACAATAAAGAGAAAAAAAATTAACGAAATAAATAAGAATAGAACTG *F AATATAATGTCGACACCAGATACAGAAAAGGACCATTACAAAAAGGAAAA *F TTAGAAGCCCCTTTCAAATTAACAAAGAACGTCGAACAAACCGACGAAGA *F CCATTACAAAATTACTAACAGAAACAGAGAGACACATTACTATAAGACCC *F AATTTAAAAAACAGAAGAAAACTAATCAACTCCCCATTTTACAGGCCCCT *F TGTTCACCATAATACTTTTTCTTACTCTGACAAACCTATTCAAATCCCTT *F CAGAATACGAACACCACTGTCTCCGCATAAACCTCTTGGATATAGACAAC *F CTAACTCACTATTTTAACACTAAAGTAACCAACTATACACATATCCCCCA *F AATTAAACTTCTACACAATAAAATGTTAAGGGAACTTAACGCTATAACGT *F TACACCAGACAAATAGACAGAAACGCGGACTAATCAACATAATGGGATCT *F GCATTTAAATACCTTTTCGGGACACTAGACAATAACGACCGGATACAAAT *F TCATAACCAATTAGAGTCCGCGACAAACAACTCAATTAACATACACGAAA *F TGAGCGACATAATTCAACTTATCAATAGTAACATGCAAAAGATCAAAGAA *F TTTGAAGACGAACATAATAACAGAGAACAAATGCTATACGAACTAATACA *F ATTTACCGAATACATTGAAGACATCGCAATGGGAATGCAACTCTCACGAC *F TAGGACTGTTTAACCCAAAATTACTTAACTATGACAAATTAGAAAACGTT *F GACAGTAGCAACATACTACAGACTAAAACCTCAACCTGGATCAACACTAA *F CCAACTACTTATCATAGCTCATATCCCTACTAAACAAAAAACCATTCATA *F CTATTAACATAATACCCTATCCAGATAATAATGGCTATCAACTTGATCAT *F ATAGACAAAGACACTTATTTCGAAGAACAAGATAAAATTTATAACCAGAA *F CTATCAAGAAATTAACAACGAATGTATCAAAAATATTATTAAGAGAACAA *F ATCCAATATGTAACTTTGTCCCTATAACAGTCGAACAAATAATTAAGTAT *F GTAGAACCAAATAGTATCATCACTTGGAATCTAAACAACACTATCATAGA *F ACAAAAATTGCCAAGAAATAAATAAAAATGTAACGGTTAACGGCAACAAA *F ATAATAACAATAAAACAATGTAAAATCAAAATAGGAAACATAATACTTAA *F CGAAAACAAGCTAAAAACCCCGAGATAAACCTTACCCCATTGTACACACC *F CTTAAGCCTAATAAAAATAAAACCAATAGAACATAAGGACATTGTACAAT *F TAATATCAAATAATAATATCACAGTTTGTATAGTAGTATCAATCATAAGC *F CTCACATCTTGTATTGCTTGTATTTACTTAAAACTAAAAAACAAAATAAT *F CCTTGAATCACAAGATGCAAATCCAACTGCATCACCAAGATTGCGGGCAT *F CAACACCAATAGAAGGAGTGGAAGCATAAGCTTCCCTTTTAAAGGGAGGG *F AAGTAACATAATATGCTTCTCATATTACGTTTACATACTTACACTAATTG *F TACATACAATCTTGCACATGCATAAACACATGAAACCAGTTTACATTTTT *F ACTTACACTTAAGCGCATAATTTGTTGTGCATCCATACCGTTATTTTTCC *F GTTCTTTTTTGTACACATATACTGATTAGACATTCCCGTTTCTCGCGACT *F CACTTCGAGCCGATCAAAAACTCTGTACAGTCAGTCTTAAGCCGACAACG *F AAGAAATAAAGATCTAAACTAAAAAATACCTCGTGTTGATTCTGAAACTT *F CTTTAAAGGCGTTGATCTTAGTCAAACGACGGATCATTTGTTCGACTCGA *F ATAGTAAAATACGTAAGT # *U FBrf0151631 *a Rogers *b G. *t 2002.8.14 *T personal communication to FlyBase *u *F Date: Tue, 13 Aug 2002 13:45:12 \-0400 *F To: gm119@gen.cam.ac.uk *F From: Greg Rogers *F Hi Gillian, *F My name is Greg Rogers, I'm a postdoc with David Sharp at the Albert *F Einstein College of Medicine. I used to work for Jon Scholey and he *F forwarded your e-mail to me. I was surprised and impressed with you *F question, you guys really keep up on this stuff which is *F fantastic. Anyway, the real story and answer is that there are actually *F two kinesin motor genes near this position one at KLP59C (CG3219) and one *F at KLP59D (CG12192), they are approximately 17Kb away from each other. The *F searching in FlyBase does recognize CG12192 as a kinesin motor at 59D1 *F position, so we're calling it KLP59D. The CT number for KLP59D is CT9848 *F but there are some mistakes I believe. Anyway, KLP59D is a real gene that *F is transcribed, and have sequenced two identical full-length ESTs for *F KLP59D (AT18571 and AT12557). Let me know if you want cDNA sequence for *F KLP59C and KLP59D \- we have them for both genes. *F Take care, *F Greg *F Dear Dr. Scholey, *F > *F >I am curating your abstract for FlyBase: *F > *F >Rogers et al., 2000, Molec. Biol. Cell 11(Suppl.): 569a *F > *F >Three microtubule-motors, cytoplasmic dynein, KLP59D and KLP38B, *F >function cooperatively to position chromosomes relative to spindle *F >poles in Drosophila early embryos. *F > *F >You mention 'KLP59D' in this abstract. We don't have a record of this *F >gene in FlyBase, but we do have a record of a 'Klp59C' (which *F >corresponds to the gene prediction CG3219). Is your 'KLP59D' the same *F >gene as 'Klp59C' ? If it is not the same gene, do you know which of *F >the Genome Project CG annotations your gene corresponds to, so that I *F >can rename the CG in FlyBase, *F > *F >thanks, *F > *F >Gillian *F Date: Wed, 14 Aug 2002 10:28:42 \-0400 *F To: Gillian Millburn (Genetics) *F From: Greg Rogers *F Subject: Re: KLP59D *F Hey there, *F Making the e-mail a personal communication would be just fine. I've *F included the KLP59C and 59D cDNA sequences attached as MSWord format *F files. If you have trouble opening them or anything, please let me know. *F Greg *F .. *F KLP59C cDNA Sequence (also know as CG3219) *F ATGGATAAGTTGTCGATCGAACAGAAGATTTTCATCCGTCGCTCGGACGGTAGAGTCCACTTGGCGGAGATTATCAAAC *F TGGAGGGCGGTGACTCCAAGCTGATTACGGTGGAGTGGCCCGAAGGACATACGGTGCGTGGTAAGGAACTGCCCCTGGA *F GCTGGTGGTCCTGATGAATCCTCATATCTTCGACTCGCCCCGCTGCAGTGGCGGCAATGCGGCGTCAGCCAATCAAACT *F GCCTCCATATCGCCACGTTCAATGAAACAACGAATAGCGACTGGGAGCTTATCTCCTGTACTGGCCACTGCCCCGCCAC *F GCCAGCAGACGGCGCCGCCAGTTCGAGAGGACGAGGTGGTGCACCAGGCTGAAAGAATGCGAAAAGAACGGGAACGGAG *F GCGAGAAGCACAAGCCAGGACTCGTCTAGATCGGGAGCAGGGGAAGAACGAAGATCCGGGAAATCCCAACTGGGAAGTA *F GCCAGAATGATACGACTGCAACGCGAGCAAATGGAGAGTCAGCGGGTGAGAAGTGGTACTACGAACGAACGAATCAATT *F GCCACCAAATTATGGTTTGTGTGAGGAAGAGACCACTGAGGCGCAAGGAGCTGGCTGACCGGGAACAGGATGTGGTCAG *F CATTCCGTCTAAGCACACATTGGTGGTCCACGAGCCCCGCAAGCATGTGAACCTGGTCAAGTTCCTGGAAAATCATAGC *F TTCCGTTTCGATTACGTCTTCGACGAGGAGTGCTCCAATGCCACGGTCTACGAATTCACAGCCCGACCCTTGATAAAGC *F ACATTTTTGATGGCGGAATGGCCACGTGTTTCGCCTACGGACAAACTGGAAGCGGCAAGACCTATACGATGGGTGGTCA *F GTTCCCCGGAAGGCATCAGAGCTCAATGGATGGCATCTATGCAATGGCCGCTAAGGACGTGTTCTCCACTCTAAAGACG *F GTTCCCTATAACAAGCTTAATCTGAAAGTTTACTGCAGCTTCTTCGAGATCTACGGCACCCGGGTGTTCGATCTCCTGA *F TGCCTGGCAAGCCACAACTGCGTGTCTTGGAGGATAGAAACCAGCAGGTGCAAGTGGTGGGCCTCACCCAGAATCCAGT *F ACAGAACACCGCCGAAGTTCTGGACCTACTCGAGTTGGGCAATAGTGTCCGAACCTCGGGTCACACCTCTGCCAATTCC *F AAGTCCTCCCGATCGCATGCTGTGTTCCAAATCGTGCTGAGATCCGCGGCGGGCGAGAAGCTACACGGGAAATTCTCGC *F TTATAGATCTGGCCGGGAATGAAAGAGGAGCGGACAACAGCTCGGCGGATCGACAGACGCGCCTGGAAGGATCCGAGAT *F CAATAAATCGCTGCTGGTCCTCAAGGAATGCATTCGCGCTCTGGGGCGCCAGTCGAGTCATTTGCCATTCCGTGGCTCC *F AAGCTGACCCAAGTCCTGCGGGACTCCTTCATCGGAGGTAAGAAGGTGAAAACCTGCATGATAGCCATGATCTCGCCAT *F GCTTGCATTCGGTGGAGCATACCTTGAACACGCTGCGTTATGCGGATCGGGTGAAGGAACTAAGTGTGGAGTCGATCCC *F ATCTAAACGGATGCCCGATGCCAACCTTGGAAGCACTTCCATGTCAGATATTGTTTGCCAGTCGTCCACCCAGCGACTC *F TTTCCATGTGCCTCCTCCACATCGATGCCAGGTGGTGGCAACCAAGCGCAGCAACACACCAACACCGCGAACGACTTGA *F ACAGATCCCAGAAACCAACATCTAAGCCCACTTATCCCACATCCGGTCAGCAGCTGGTCCAGAGGAAGGGAAGCTCCCA *F ACGGGAGGCCTCCATGATGCTGACCAAATCCCTGGCACAATTCCGAGGGCGCAACTTTCCCTAA *F KLP59D cDNA Sequence (also know as CG12192) *F ATGGATCGCATCAAAATTGGCGAGCAGCTCCTCTTCCAGAGGAGTGACGGGCGTGTCCAT *F CAGGTGGTCTGCACTGCCAAGAACCTGGAACGGGATTCCATCACGGGCGAATGGACCGAG *F GGCACGGTGGTGAAGGGTAAGGAAGTGCCATTGAGCACGCTGATTGGCATCAACCATCAT *F ATATTCGCGGAGAATCAACCGCCGATATCGAAGCCACCGAGCTCCTTGCTGCCGAAGCCT *F CGAGGCTCGAGTCCGACCGGAGGACCTAAGACCCACACTGGAAATCCGTATGCAGAGCGC *F ATGAGAGTACAGGACGGTCGCAGTAAAATTGCCACCAGAAAGACGGATCCTGCAGCGGTC *F GGAGCGGGAAATCATGAGCTGGGTAGAGCGCACACCCCTCGTGTGCCAGCCGGTAGGCCG *F GACCGGGATGCCAGCCCCACCCAAGGTCGCAAGTCACAGGGTGGCAACAATAGCAACCAA *F CGCTTTTCCAGTGTAGTGAGGGAGGTGAATCGCATGAAGGAGCAGAGGGAGAAGCGAAGG *F GCTCGCCAGGCGGAACAGCTCCAGGAGAAGGATGCACTGCGTCGCAATAATCCGGGGAAT *F CCCAACTGGGAGGTGCACTGTTCAGCAAATTACGGTGTGTGTGCGAAAACGACCCATGAG *F TCGCAAGGAGGAGAACTCCAAGAACCTGGACATCATCACAGTTCCCAGTGCCGACAGCCT *F GATCGTCCATGAGTTGCGCCTCAAGGTGGATCTCACCAAGTTCCTGGAGCACCACAAATT *F CCGTTTCGACTACACGTTCGACGAGGAGTGCTCCAATGCGCTGGTCTACGATCACACTGC *F TCGTCCGTTGATCAGAACCATGTTCGAGGGCGGCAATGCCACTTGTTTCGCTTACGGACA *F AACTGGCAGCGGAAAAACGCACACCATGGGCGGAGAATTCTTCGGAAAGGTTCAGGATTG *F CGGTACCGGGATCTACGCCATGGCAGCTCGCGATGTCTTCGAGGAGGTATCGCGCCCGGA *F GTACCGGCAAATGGGTGCCAAGATTACGTGCAGCTTCTTCGAAATCTATGGCACCAAGGT *F GTTCGATCTCTTGCTACCCAACAAGCCCATGCTGCGGGTCCTAGAGGATGCCAGGCAGCA *F GGTCGTGGTGGTGGGCCTAACGGAGATGCCGGTGACCAAAGTGGAGGATGTCCTGAGACT *F GATTGAGCACGGCAGCAAAGAGCGCACTTCCGGCCAAACATCGGCGAACGCCAAGTCATC *F GCGTTCCCACGCCGTCTTTCAAATAGCACTCCACTTTCCCGATTCCTGGGGCCCACACGG *F CAAGTGCTCCTTTGTGGACTTGGCGGGCAATGAACGCGGGGCGGATACGCAATCCGCCGA *F TCGTCAAACTCGCATCGAGGGAGCCGAGATCAATAAATCTCTGCTGGCCCTCAAGGAGTG *F CATTCGAGCCCTCAGCCGGCAGTCGAGTCACCTTCCCTTCCGTGGCTCCAAGTTGACCCA *F AGTGCTGCGCGACTCCTTTGTCGGCGGCAAGAAGAACAAGACCTGCATGATTGCCATGAT *F ATCGCCATCCATGAGCTGCGTGGAGAATACGCTCAACACTCTACGTTACGCAGACAGGGT *F TAAGGAGCTCATAGCCAAGGAAGACGAACACTTGCAGTCCGTGGAAGGGGATGGAGAGAA *F GTCTCCCGATCTCAACGAGGAATCAGAGCCAGAAATGATGGCCGACGAGGAGGGCGATGA *F GGAGCCGGAGGATGAGGACAATCAGCATCTAACCATATCCTCGGAGGAGGCAAGCTCCTA *F CAACAACATGAGTTATGACATGAGCTTTAACCACACCCTCAACATTCTGGGTCCCTCAAG *F GAACGTGGATATCCAAGAGGTGGCCGAGCAGCACGCACTTTTGGTAGAGAATCTAGAGAC *F TTACGCTCACAATTTCCGGCAACTGAAAACGGATAAGGAAATAGAGCAGTACACGCAAAA *F TTCAGAAAGCGCATTGATGAAACTGCTAGCAATGGTGAATAGAACGCGGGATGTAACGCA *F CAATTACAACACTCAGAAATTATTGAAGGAAGAAAACCAAAATGCATATAAGAAGGGCGA *F GCTGGATGAATCCGAGTAG # *U FBrf0151643 *a Deal *b J. *c K. *d Cook *t 2002.8.20 *T personal communication to FlyBase *u *F Date: Tue, 20 Aug 2002 11:00:41 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC20 *F Isolation and characterization of Df(3L)BSC20 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC20 was isolated as a P transposase-induced male recombination *F event involving P{PZ}l(3)0086400864 and P{lacW}l(3)L3809L3809. The *F deletion was isolated as a st1-ca1 recombinant chromosome from the *F cross st1 Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; *F st1 P{PZ}l(3)0086400864/P{lacW}l(3)L3809L3809 ca1 males. The *F miniwhite marker in P{lacW}l(3)L3809L3809 was disrupted or deleted. *F Polytene chromosome squashes showed the breakpoints 76A7-B1;76B4-5. *F Df(3L)BSC20 failed to complement pip2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151644 *a Deal-Herr *b M. *c K. *d Cook *t 2002.8.20 *T personal communication to FlyBase *u *F Date: Tue, 20 Aug 2002 11:12:17 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC21 *F Isolation and characterization of Df(3L)BSC21 *F Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC21 was isolated as a P transposase-induced male recombination *F event involving P{PZ}Ten-m05309 and P{A92}nrmA37. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsdd-1 es ro1 ca1 females X CyO, H{PDelta2-3}HoP2.1/+; ru1 h1 *F P{A92}nrmA37 sr1 es ca1/st1 P{PZ}Ten-m05309 ry506 *F males. Polytene chromosome squashes showed the breakpoints 79E5-F1;80A2-3. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151645 *a Ranson *b H. *t 2002.8.27 *T personal communication to FlyBase *u *F To: HRanson@liverpool.ac.uk *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Wed, 21 Aug 2002 15:57:54 \+0100 *F Dear Dr. Ranson, *F I am curating your paper for FlyBase: *F Ranson et al., 2001, Biochem. J. 359: 295--304 *F In this paper you compare insect GSTs, including Drosophila ones. *F You say in the Materials \+ Methods that: *F 'Two putative full-length transcripts were dervied from the annotations *F for CG12930 and CG6673. The derived amino acid sequences of both of *F these were included in this study (denoted by A and B)'. *F Do you have the amino acid sequences of CG12930A, CG12930B, CG6673A and *F CG6673B ? *F If you could send me the sequences I would be able to figure out which *F of the transcription units in FlyBase these correspond to \- the *F structure of the CGs is changing as Release 3 of the genome annotation *F is just about finished (for example 'CG12930' has been split into 2 *F separate genes), so I'd like to be able to figure out which of the new *F release 3 transcripts your amino acid sequences correspond to if *F possible, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph: 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F Date: Tue, 27 Aug 2002 14:28:01 \+0100 *F To: Gillian Millburn (Genetics) *F From: Hilary Ranson *F Subject: Re: FlyBase query *F Dear Gillian, *F Sorry for the delay in replying to your request. I have pasted the *F putative amino acid sequences of the Drosophila GSTs, CG12930A and B *F and CG6673A and B below. I have also included the other putative GSTs *F whose amino acid translations were modified from those contained in *F release 1 of flybase. The modifications are described in the *F materials and methods of the same Biochem J. manuscript. *F .. *F please note these annotations were done based on *F alignments and studying of intron exon boundaries. The putative cDNA *F sequences have not been confirmed experimentally. *F Please let me know if you need any further information. *F Hilary *F >CG12930B *F mskaikyyyd flsqpsralw iamklgktpf edcpvalrkq eqltdeyrsi nrfqkvpaiv *F dgkfqlgesv sivryladkg vfseqlypkt leerarvdef lewqhfnvrl *F vcslffrqvw llpakglapa pkpesvkkli *F kdvesnlgllerlwlelkdflvgdkltvadifgsseinqynvnekqfpkvakwmerdatnpyydeahsfvyktsqq *F avkakn *F >CG12930A *F mskpirfyyd llspiarglw iglkfsnspv *F eycpialrkf eqltdeykki nrfqkvpaiv ggdfhlseti aiiryladkg qfdeklypkt *F lenrarvdef lewqhlnirl acsmyfrdaw lfpmngiapk pkpeqiqali egvennlgll *F erlwlendfl vgknltmadi *F lgsseinqrvdekkfpkvvkwlervrvsanpyhdegltfidrkskqstaakl *F >CG1702 *F msapiryyyd lmsqpsralf *F iifrlsnmpf edcvvalrng ehltedfkke inrfqrvpci hdngyklaes vailrylsak *F gkipehlypk yfvdqsrvde flewqhmslr ltcamyfrtv wleplltgrt pseakietfr *F mqmernldvv eevwlegkdf ltgssltvad ifaaceieqt rmadydvrik ypkirawlkr *F vrqscnpyyd vahefvykis gtgpqakl *F >CG17639 *F msppvlyyl ppsppcrsil llakmldidf elkivnileg *F eqlkpdfvam npqhcvptmn deglvlwesr ailsylvaay gksdqlyptd irvralvdqr *F lqfdlgtlym rltdyyfptm figapldegk raklaeavgw lntilegrqf saadhftiad *F ltllvtvsql eafefelrpy khirqwldrc kdhmapfdye elnankanml admfkakmnq *F sag *F >CG10065 *F mklyavsdg ppslavrmtl kaldiqyqli *F nvdfcamehr seeyskmnpq keipvldddg fylsesiaim qylcdkyapd stlypqdvnv *F ravinqrlcf nmgfyyapis ahsmapiffd ykrtpmslkk vqnaldvfet ylqrlgtkya *F agenitiadf alisaticle ainfdlhqft lvnkwyetfk veypqlweia nsgmqeisaf *F eqnppdmshm ehpfhptrks mglkl *F >CG6673A *F malpqkhfkrgspkpeipedgvlryysmrfcpysqraglvlaakkiphhtvyidlsekpewyidysplgkvpaiqlpnlp *F gqpalveslviaeyldeqypgegslfpkdplqkaldrilierlspavsaiypvlftknppadaiknfetaldvfeqeitk *F rgtpyfggnkigiadymiwpwferfpalkytldepyeldktryqnllkwrdlvaqdeavkataldarihakfmktrhenk *F pdydvafqpl *F >CG6673B *F mlflpfqlgfgstkpelpedgvprffsmafcpfshrvrlmlaakhiehhkiyvdliekpewykdfsplgkvpalqltgvk *F dqptlvesliiaeyldqqypqtrlfptdplqkaldkilierfapvvsaiypvltcnpnapkdaipnfenaldvfevelgk *F rgtpyfagqhigivdymiwpwferfpsmkinteqkyeldtkrfekllkwrdlmtqdevvqktaldvqlhaefqksktlgn *F pqydiafkgtp *F >CG1681 *F msqplkfyfdflnqssralyilleaskipfeaipismlgehltgefrdnvnrfrklpaitdhgyqlsenvaifrhlarek *F lvpehwyprrhlgrsrideylawqqtnmgvatteyfqqkwlvpylqktrpadnavnlaskqlehtlnefeqlflnsrkfm *F mgdnisyadlsaiceidqpksigynafqnrnklarwyetvreelgphykevlgefeaklkgsgsgqqqgvaqavkq # *U FBrf0151649 *a Suzuki *b T. *t 2002.9.13 *T personal communication to FlyBase *u *F Date: Fri, 13 Sep 2002 18:11:36 \+0900 *F To: Gillian Millburn (Genetics) *F From: Toshiharu Suzuki *F Subject: Re: FlyBase query: Aplip1 *F Yes, dp1 is APLIP1. *F Thank you. *F At 9:51 AM \+0100 02.9.13, Gillian Millburn (Genetics) wrote: *F >Dear Dr. Suzuki, *F > *F >I am a curator working for FlyBase and I have a question about a gene *F >mentioned in your conference abstract: *F > *F >Yagi et al., 1999, A. Dros. Res. Conf. 40: 560A *F > *F >Analysis of Alzheimer related genes in Drosophila. *F > *F >In this abstract you mention a clone named 'dp1': *F > *F >'At the same time, we tried yeast two-hybrid screen to get APPL *F >cytoplasmic domain binding proteins, and recovered clones. One clone *F >tentatively named dp1 can bind APPL cytoplasmic domain thorough PI *F >domain. But no known APP binding proteins showed homology with dp1. dp1 *F >is also expressed in CNS of embryo. dX11L was also recovered from the *F >screen and confirmed APPL binding activity.' *F > *F >I am writing to ask whether or not 'dp1' is the same gene as 'APLIP1' *F >(APP-like protein interacting protein 1) which you describe in your *F >paper: *F > *F >Taru et al., 2002, J. Biol. Chem. 277(22): 20070--20078 *F > *F >If they are the same gene then I will be able to merge the information *F >in FlyBase currently under 'dp1' into the APLIP1 gene record, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph: 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F \-- *F \---------------------- *F Toshiharu Suzuki, Ph.D *F Professor & Head *F Laboratory of Neuroscience *F Graduate School of Pharmaceutical Sciences *F Hokkaido University *F Sapporo 060-0812 JAPAN *F Tel: \+81-11-706-3250 (Office) *F \+81-11-706-3251 &Bgr;!' (B3252(Lab) *F FAX:+81-11-706-4991 *F e-mail: tsuzuki@pharm.hokudai.ac.jp *F http://www.hokudai.ac.jp/pharma/shinkei/index.html *F \----------------------- # *U FBrf0151650 *a Giraldez *b A.J. *t 2002.9.13 *T personal communication to FlyBase *u *F To: Gillian Millburn *F Subject: Re: FlyBase Help Mail *F Date: Tue, 27 Aug 2002 16:31:46 \+0200 (CEST) *F From: 'Antonio J. Giraldez' *F Dear Gillian, *F Please find the answer to your questions. *F If I've missed something or you need more information, I'll be happy to answer *F you. *F Best regards and thanks for your interest in curating this information. *F Antonio. *F Quoting Gillian Millburn : *F > Dear Antonio, *F > *F > I have finished curating your Dev. Cell paper on Notum (Dev. Cell 2(5): *F > 667--676), and I have some questions about the various mutants and *F > constructs you used. *F > *F .. *F > *F > You state that 'Notum4' ( = superscript) is l(3)72Da. *F > *F > We have a record of 4 l(3)72Da alleles in FlyBase \- which one did you *F > use ? *F > *F > Did you get the l(3)72Da allele from the Bloomington Stock Center \- if *F > you did what is the Stock number ? *F > *F Stock Number: 5051 *F Genotype: l(3)72Da1/TM6C, cu1 Sb1 ca1 *F All the alleles obtained from the stock center contained second side lethal *F mutations. The stocks used in my paper were cleaned *F this also applies for Notum5, which is viable in homozygosis. *F Stock Number: 4117 *F Genotype: w1118?; l(3)72CDfI6 P{w+m*=*}71F/TM3, Sb1 *F .. *F Antonio J. Giraldez PhD. *F Stephen Cohen's Lab *F EMBL, Meyerhofstrasse 1 *F Heidelberg. Germany *F Phone \+49 6221 387 290 *F Fax \+49 6221 387 166 *F Home \+49 6221 656 586 *F To: giraldez@embl-heidelberg.de *F Subject: Re: FlyBase Help Mail *F From: Gillian Millburn (Genetics) *F Date: Tue, 10 Sep 2002 10:32:27 \+0100 *F Dear Antonio, *F sorry for the delay in replying. Thanks for your answers to my *F questions about your Dev. Cell paper on Notum, they were very helpful. *F .. *F 1. You say in your reply that 'Notum4' (l(3)72Da1, stock 5051) has a *F second site lethal. I need to know whether or not the cleaned *F 'Notum4' allele is lethal when homozygous or over a deficiency that *F removes Notum \- i.e. is there lethality associated with the Notum *F mutant in this allele or is the only lethality due to the separable *F second site lethal. At the moment in FlyBase we have a separate gene *F record for 'l(3)72Da' and I need to know whether or not to merge this *F lethal complementation group in with Notum or keep it as a separate *F lethal gene (which corresponds to the second site lethality). *F 2. Also you say that Notum5 is homozygous viable. *F In the paper: *F Gerlitz and Basler, 2002, Genes Dev. 16(9): 1055--1059 *F they say that Notum5 is a pupal lethal as a homozygote and also is *F lethal over Df(3L)st-f13. So I am wondering: *F Is Notum5 lethal over a deficiency that removes Notum ? i.e. is it a *F hypomorph that is homozygous viable but is lethal over a deficiency for *F Notum because less Notum product is present in the hemizygous case. *F OR is it lethal over Df(3L)st-f13 because this Df removes the locus *F responsible for the secondary lethal. Again, I need to know this to *F establish whether there is any lethality associated with Notum in the *F Notum5 chromosome, or whether all the lethality is due to a separate *F second site lethal. *F 3. Finally, if the lethality in Notum4 and Notum5 is all due to *F secondary lethals i.e. there is no lethality associated with the *F mutation of the Notum gene in these alleles, do you know whether the *F second site lethals in Notum4 and Notum5 are in the same *F complementation group ? *F sorry to ask such a complicated question, but it would really help sort *F things out properly in the database if you could help with any of this, *F thanks *F Gillian *F > > *F > > You state that 'Notum4' ( = superscript) is l(3)72Da. *F > > *F > > We have a record of 4 l(3)72Da alleles in FlyBase \- which one did you *F > > use ? *F > > *F > > Did you get the l(3)72Da allele from the Bloomington Stock Center \- if *F > > you did what is the Stock number ? *F > > *F > *F > Stock Number: 5051 *F > Genotype: l(3)72Da1/TM6C, cu1 Sb1 ca1 *F > *F > All the alleles obtained from the stock center contained second side lethal *F > mutations. The stocks used in my paper were cleaned *F > *F > this also applies for Notum5, which is viable in homozygosis. *F > Stock Number: 4117 *F > Genotype: w1118?; l(3)72CDfI6 P{w+m*=*}71F/TM3, Sb1 *F > *F > *F To: Gillian Millburn *F Subject: Re: FlyBase Help Mail *F Date: Fri, 13 Sep 2002 10:47:32 \+0200 (CEST) *F From: 'Antonio J. Giraldez' *F Dear Gillian, *F Thanks for your interest, please find the answer to your questions below. *F Quoting Gillian Millburn : *F > Dear Antonio, *F > *F > sorry for the delay in replying. Thanks for your answers to my *F > questions about your Dev. Cell paper on Notum, they were very helpful. *F > *F > *F > 1. You say in your reply that 'Notum4' (l(3)72Da1, stock 5051) has a *F > second site lethal. I need to know whether or not the cleaned *F > 'Notum4' allele is lethal when homozygous or over a deficiency that *F > removes Notum \- i.e. is there lethality associated with the Notum *F > mutant in this allele or is the only lethality due to the separable *F > second site lethal. At the moment in FlyBase we have a separate gene *F > record for 'l(3)72Da' and I need to know whether or not to merge this *F > lethal complementation group in with Notum or keep it as a separate *F > lethal gene (which corresponds to the second site lethality). *F The stock 5051 has a second site lethal because over itself it is embryonic *F lethal but over the deficiency Df(3L)st-f13 or Notum alleles is larval-early- *F pupal lethal. also the Notum alleles that I isolated are, once cleaned, larval- *F early-pupal lethal over the Df(3L)st-f13. I would say that they are one *F complementation group. *F Notum complements with stock 5052 *F l(3)72Db8/TM6C, cu1 Sb1 ca1 *F If you want to send me some of the other alleles l(3)72Da, I can do a *F complementation test with Notum. *F > 2. Also you say that Notum5 is homozygous viable. *F After cleaning it, I have been able to generate a semi-lethal stock that shows a *F strong phenotype in homozygosis. This stock can be kept in homozygosis. Many *F pupae die. *F > *F > In the paper: *F > *F > Gerlitz and Basler, 2002, Genes Dev. 16(9): 1055--1059 *F > *F > they say that Notum5 is a pupal lethal as a homozygote and also is *F > lethal over Df(3L)st-f13. So I am wondering: *F I can get viable flies Notum5 over Df(3L)st-f13 but very few. The phenotype is *F stronger than Notum5 homozygous. *F > *F > Is Notum5 lethal over a deficiency that removes Notum ? i.e. is it a *F > hypomorph that is homozygous viable but is lethal over a deficiency for *F > Notum because less Notum product is present in the hemizygous case. *F Yes I would say it is the case. *F > *F > OR is it lethal over Df(3L)st-f13 because this Df removes the locus *F > responsible for the secondary lethal. Again, I need to know this to *F > establish whether there is any lethality associated with Notum in the *F > Notum5 chromosome, or whether all the lethality is due to a separate *F > second site lethal. *F In all the cases I've look at, Notum mutants are lethal from embryonic to *F larval- *F early pupae. Except when one of the alleles is Notum5 then I get some flies *F and the number is under mendelian ratios. *F > *F > 3. Finally, if the lethality in Notum4 and Notum5 is all due to *F > secondary lethals i.e. there is no lethality associated with the *F > mutation of the Notum gene in these alleles, do you know whether the *F > second site lethals in Notum4 and Notum5 are in the same *F > complementation group ? *F No, the second site lethals are different because in transheterozygosis they *F show *F a lethality that goes form the embryo to the pupae, getting some adults ( *F because *F Notum5 from my point of view is a hypomorph. This contrasts with the sequence *F data of Notum5 that appears as a stop codon at the beginning of the protein. *F (there might be another starting codon after?) *F > *F > sorry to ask such a complicated question, but it would really help sort *F > things out properly in the database if you could help with any of this, *F > *F > thanks *F > *F > Gillian *F > *F > *F > > > *F > > > You state that 'Notum4' ( = superscript) is l(3)72Da. *F > > > *F > > > We have a record of 4 l(3)72Da alleles in FlyBase \- which one did *F > you *F > > > use ? *F > > > *F > > > Did you get the l(3)72Da allele from the Bloomington Stock Center \- *F > if *F > > > you did what is the Stock number ? *F > > > *F > > *F > > Stock Number: 5051 *F > > Genotype: l(3)72Da1/TM6C, cu1 Sb1 ca1 *F > > *F > > All the alleles obtained from the stock center contained second side *F > lethal *F > > mutations. The stocks used in my paper were cleaned *F > > *F > > this also applies for Notum5, which is viable in homozygosis. *F > > Stock Number: 4117 *F > > Genotype: w1118?; l(3)72CDfI6 P{w+m*=*}71F/TM3, Sb1 *F > > *F > > *F > *F Antonio J. Giraldez PhD. *F Stephen Cohen's Lab *F EMBL, Meyerhofstrasse 1 *F Heidelberg. Germany *F Phone \+49 6221 387 290 *F Fax \+49 6221 387 166 *F Home \+49 6221 656 586 # *U FBrf0151651 *a Hekmat-Scafe *b D. *t 2002.3.5 *T personal communication to FlyBase *u *F Date: Tue, 5 Mar 2002 15:15:21 \-0800 *F From: Daria Hekmat-Scafe *F To: sima@fruitfly.berkeley.edu *F Cc: Mark Tanouye , daria@nature.berkeley.edu *F Subject: OBP Gene Family *F Sima, *F I have identified a group of 51 Drosophila genes which likely encode a *F family of odorant-binding proteins (OBPs). Below I have given the *F corresponding nucleotide and amino acid sequences for the coding regions of *F each of these genes, along with the genes' tentative cytological locations. *F Because the number of OBP genes is large and because family members have *F previously been known by several conflicting names, I am proposing a single *F nomenclature analogous to the one used for the large family of Drosophila *F odorant receptor genes (Drosophila Odorant Receptor Nomenclature Committee *F 2000 Cell 102:145). The previous names (given in parentheses) include: *F PBPRP (pheromone-binding protein related protein gene) (Pikielny et al. *F 1994 Neuron 12:35), OS (olfactory-specific gene) (McKenna et al. 1994 JBC *F 269:16340), LUSH (Kim et al. 1998 Genetics 150: 711), and OBP *F (odorant-binding protein gene) (Galindo and Smith 2001 Genetics 159:1059). *F I have modified the annotations to some of the CG and OBP genes as *F indicated by the suffix '.m'. Please note that Obp83e is composed of two *F tandem, in-frame OBP-like sequences following a single signal sequence. *F Four of the Obp genes (8a, 44a, 99b and 99c) encode atypical OBP-like *F proteins that lack the second and fifth of six conserved cysteine residues. *F Daria *F Obp8a (CG12665.m) 8D34 *F ATGGCCCATTGTATGTACATTCTGCTGCTGTTGCTGCTGGTGGTGGAACTGACGCCCCCTGCTATTCCGGTGCCCATGCG *F ATCCTCACCCCAATCGCTGGCCCTACTGCGAGCACGGGATCAGTGCGGCAGGGAGCTGACTGCTGCCCAGCGTCTGCAGC *F TGGACAGGATGCAATTCGAGGATGCTGCCCATGTGCGTCACTATCTCCATTGCTTCTGGTCACGGCTGCAGCTCTGGCTG *F GATGAGACCGGATTCCAGGCACAGCGCATCGTTCAGAGTTTCGGCGGCGAGAGGCGTCTCAATGTGGAGCAGGCACTGCC *F AGCCATCAACGGGTGCAATGCGAAAACGAGCTCCAGAGGATCGGGCGCTCAGACAGTGGTCGACTGGTGTTTCCGTGCCT *F TTGTCTGCGTGCTGGCCACTCCAGTCGGTGAGTGGTACAAGCGCCACATGTCCGATGTCATCAATGGGAATGCC *F mahcmyillllllvveltppaipvpmrsspqslallrardqcgreltaaqrlqldrmqfedaahvrhylhcfwsrlqlwl *F detgfqaqrivqsfggerrlnveqalpaingcnaktssrgsgaqtvvdwcfrafvcvlatpvgewykrhmsdvingna *F Obp18a (CG15883.m, OBP18a.m) 18B1 *F ATGAAGGTTGTGTGCAGCATAGCTGTACTATGGATTTGCTTGATAACTATGTGGCAATCAGCTGGCCGCGTTAACGCAGA *F GGGTTGCCTAAAGCACCACAATCTGACCAGTGCCCAAGTGCAGGCAGTGGCTCCATCCACTCCCGTTGCGGATGTTCCAG *F TGGCCGTTAAGTGCTATAGCCGGTGTCTGATCCAGGATTATTTCGGTGATGATGGGAAAATCGATCTGCAGAAGGTGGGA *F AAGCGAGGATCTCAAGAGGACCACGTGATTTTGTCCCAGTGTAAGCAGCAGTTCGATGGCGTCACCAATCTGGACACGTG *F CGACTATCCATACCTGATTCTCCAGTGTTATTTTAAGGGCAAGCAGAGTGGAACTATCGCCTCG *F mkvvcsiavlwiclitmwqsagrvnaegclkhhnltsaqvqavapstpvadvpvavkcysrcliqdyfgddgkidlqkvg *F krgsqedhvilsqckqqfdgvtnldtcdypylilqcyfkgkqsgtias *F Obp19a (CG11748.m, OBP19a) 19D2 *F ATGAAGTTCCATCTGCTGCTGGTCTGCGTCGCCATATCCCTGGGACCAATCCCCCAGTCGGAGGCAGGGGTGACGGAGGA *F GCAGATGTGGTCTGCCGGAAAGCTGATGCGCGACGTCTGCCTGCCCAAGTATCCGAAGGTCAGCGTCGAGGTGGCCGACA *F ACATTCGCAACGGTGACATACCCAATAGCAAGGACACCAACTGCTACATCAATTGCATCCTGGAAATGATGCAGGCAATC *F AAGAAGGGAAAGTTCCAGCTGGAGTCGACCCTCAAGCAGATGGACATCATGCTGCCGGACAGCTACAAGGACGAGTACCG *F CAAGGGCATCAATCTGTGCAAGGACTCCACCGTCGGCCTGAAGAACGCCCCCAACTGCGATCCCGCCCACGCCCTGCTCA *F GCTGCCTGAAGAACAACATCAAGGTATTCGTGTTTCCC *F mkfhlllvcvaislgpipqseagvteeqmwsagklmrdvclpkypkvsvevadnirngdipnskdtncyincilemmqai *F kkgkfqlestlkqmdimlpdsykdeyrkginlckdstvglknapncdpahallsclknnikvfvfp *F Obp19b (CG1670.m, clot \#2470, OBP19b.m) 19D2 *F ATGACGAACCTTCTGCTAGCAGTGGCCTGCGCCGCCGTGCTGATGGGATCGGCGACGGCGGACGAGGAGGAGGGGTCCAT *F GACCGTGGACGAGGTGGTGGAGCTGATCGAGCCCTTTGGCGACGCCTGCACGCCAAAGCCGTCGAGGGAGAACATCGTCG *F AGATGGTGCTGAACAAGGAGGACGCCAAGCACGAGACCAAGTGCTTCCGCCACTGCATGCTGGAGCAGTTCGAGCTGATG *F CCCGAGGATCAGTTGCAGTATAACGAGGACAAGACGGTCGATATGATCAACATGATGTTCCCGGATCGCGAGGACGACGG *F CAGGCGCATCGTCAAGACCTGCAACGAGGAGCTAAAGGCCGAGCAGGACAAGTGCGAGGCAGCCCACGGGATCGCTATGT *F GCATGCTGCGCGAGATGCGCTCTTCGGGCTTCAAGATTCCCGAGATCAAGGAA *F mtnlllavacaavlmgsatadeeegsmtvdevveliepfgdactpkpsrenivemvlnkedakhetkcfrhcmleqfelm *F pedqlqynedktvdminmmfpdreddgrrivktcneelkaeqdkceaahgiamcmlremrssgfkipeike *F Obp19c (CG15457, OBP19c) 19D2 *F ATGAAGCCATCCACTCCAGTCGCAGCCATTCCGCTAATGACGATAGTGGTCGCTGTCCTGCTGCAGACGCACTGCGTCCG *F TGGCCAGACCCAGGCTTTCGATCTCGCCAAGCTGCTGCCCAAGACCGGAACGGAGCCCATCTGGGCGGTAATCGATCGCA *F ACTTGCCGCAGGTGCAGGAGCTGGTCACCGCGGCCAGGATGGAGTGCATCCAGAAGCTGCAGCTGCCCAGGGACCAGCGA *F CCGCTGGGGAAGGTGACCAATCCAAGTGAGAAGGAGAAGTGCCTGGTGGAGTGCGTGCTCAAGAAGATCAAGTTGATGGA *F CGCCGACAACAAGCTGAACGTTGGCCAGGTGGAGAAGCTGACCAGCCTGGTGACCCAGGACAACAAGATGGCCATCGCCG *F TTAGCTCCAGCATGGCGCAGGCCTGCAGCCGCGGCATCTCCTCGAAGAACCCCTGCGAGGTGGCCCACCTCTTCAACCAG *F TGCATCAGTCGCCAGCTGGAGCGCAACAACGTAAAGCTGGTCTGG *F mkpstpvaaiplmtivvavllqthcvrgqtqafdlakllpktgtepiwavidrnlpqvqelvtaarmeciqklqlprdqr *F plgkvtnpsekekclvecvlkkiklmdadnklnvgqvekltslvtqdnkmaiavsssmaqacsrgissknpcevahlfnq *F cisrqlernnvklvw *F Obp19d (PBPRP-2, OBP19d) 19D2 *F ATGTCGCATCTGGTTCACCTGACCGTCCTGCTCCTAGTGGGCATCCTCTGCCTGGGCGCCACCAGCGCCAAGCCGCACGA *F GGAGATCAACAGGGACCACCTTCTTGAGCTGGCCAACGAGTGCAAGGCTGAGACGGGAGCCACCGATGAGGATGTGGAGC *F AGCTGATGAGCCACGACCTGCCCGAGAGACACGAGGCCAAGTGCCTGCGCGCCTGCGTGATGAAAAAGCTGCAGATAATG *F GATGAATCCGGTAAGCTGAACAAGGAACACGCCATCGAGTTGGTCAAGGTCATGAGCAAGCACGATGCAGAGAAGGAAGA *F CGCTCCCGCCGAGGTGGTGGCCAAGTGCGAGGCCATCGAGACACCCGAGGATCATTGCGACGCTGCCTTCGCCTACGAGG *F AATGCATTTACGAGCAAATGAGGGAGCATGGACTCGAGCTGGAGGAGCAC *F mshlvhltvlllvgilclgatsakpheeinrdhllelaneckaetgatdedveqlmshdlperheakclracvmkklqim *F desgklnkehaielvkvmskhdaekedapaevvakceaietpedhcdaafayeeciyeqmrehgleleeh *F Obp28a (PBPRP-5, OBP28a) 28A1 *F ATGCAGTCTACTCCAATCATTCTGGTGGCAATCGTCCTTCTCGGCGCCGCACTGGTGCGAGCCTTTGACGAGAAGGAGGC *F CCTGGCCAAGCTGATGGAGTCAGCCGAGAGCTGCATGCCGGAAGTGGGGGCCACCGATGCCGATCTGCAGGAAATGGTCA *F AGAAGCAGCCAGCCAGCACATATGCCGGCAAGTGCCTGCGCGCCTGCGTGATGAAGAACATCGGAATTCTGGACGCCAAC *F GGAAAACTGGACACGGAGGCAGGTCACGAGAAGGCCAAGCAGTACACGGGCAACGATCCGGCCAAGCTAAAGATTGCCCT *F GGACATCGGCGAGACCTGTGCCGCCATCACTGTGCCGGATGATCACTGCGAGGCCGCCGAAGCCTATGGCACTTGCTTCA *F GGGGCGAGGCCAAGAAACATGGACTCTTG *F mqstpiilvaivllgaalvrafdekealaklmesaescmpevgatdadlqemvkkqpastyagkclracvmknigildan *F gkldteaghekakqytgndpaklkialdigetcaaitvpddhceaaeaygtcfrgeakkhgll *F Obp44a (CG2297, clot \#1214) 44A8 *F ATGAAGAACGCTGTTGCTATTCTGCTGTGCGCCCTGCTGGGTCTGGCCTCTGCCTCCGACTACAAGCTGCGCACCGCCGA *F GGATCTGCAGAGCGCGCGCAAGGAGTGTGCCGCCAGCAGCAAGGTGACCGAGGCCCTGATCGCCAAGTACAAGACCTTCG *F ACTATCCCGACGATGACATCACCCGCAACTACATCCAGTGCATCTTCGTCAAATTCGACCTGTTCGATGAGGCCAAGGGC *F TTCAAGGTGGAGAACCTGGTGGCGCAACTGGGCCAGGGCAAGGAGGACAAGGCCGCGCTGAAGGCCGACATCGAGAAGTG *F CGCCGACAAGAACGAGCAGAAGTCGCCCGCCAACGAATGGGCCTTCCGTGGCTTCAAGTGTTTCCTTGGCAAGAACCTGC *F CCCTCGTGCAGGCCGCCGTCCAGAAGAAC *F mknavaillcallglasasdyklrtaedlqsarkecaasskvtealiakyktfdypddditrnyiqcifvkfdlfdeakg *F fkvenlvaqlgqgkedkaalkadiekcadkneqkspanewafrgfkcflgknlplvqaavqkn *F Obp46a (CG12905.m) 46F11 *F ATGTGCTCCCAACTGTTCGCATTTCTGCTCCTCCTTTTGACCGCTTTTGTGACGGGTAGAAGTACTCCACCGGCGTTGGA *F TGAAGACTGTGAACTGAATTCTGTAGATACAATGCATGATTTCTGCTGCGACCTGCACGACGAGAGTCCCCAGTTTAGCG *F ACTGCCAGATGGAGTGGCACGAAAAGATTCCATACGAGACGGATGAAGAGGAGCAGACGTACATGTTTTGCACCGCAGAG *F TGCAGTTTCAACAGTACGAACTTCTTGGGCAGGGACCGCCGTTCACTGAATCTCAATGAGGTAAAGGAGCACTTGGAAAG *F CGACCTGGTCAACGATGCGGACATTAAGCTTCTCTATGACACCTATGTCAAGTGCGACAAGCATGCGCTCTCACTGATGC *F CGCACAAAGGTGTGAAGCAGCTATCCAAACGTCTTTCGCGTCTTGGCTGTCATCCGTATCCCGGACTGGTCCTCGAGTGC *F GTGGCCAACGAGATGATCCTGCACTGTCCCACCAAGCGCTTCCGTCAAACAGCCCAGTGCGAAGAGACACGCAATCATTT *F GAAGCAATGTATGCAGTATCTAAAGTACAAGTCCTAG *F mcsqlfafllllltafvtgrstppaldedcelnsvdtmhdfccdlhdespqfsdcqmewhekipyetdeeeqtymfctae *F csfnstnflgrdrrslnlnevkehlesdlvndadikllydtyvkcdkhalslmphkgvkqlskrlsrlgchpypglvlec *F vanemilhcptkrfrqtaqceetrnhlkqcmqylkyks *F Obp47a (CG12944.m, OBP47a.m) 47C6 *F ATGAATCGAGTTCTAGTGCTATTGCTGGTGCTTAAAATGTTCGCTTTGAGCGAGTTATAAAAGCACATCTTCTGTCAGTC *F CCGTTTCGCCAAGATAAACATCAATCTGGGACTAACCGTGGCTGATGAATCCCCCAAAACGATCACCGAGGAAATGATTC *F GCCTGTGCGGAGATCAAACGGATATATCCCTCAGGGAGTTGAACAAGTTGCAAAGGGAGGACTTCTCGGATCCCTCGGAA *F TCGGTCCAGTGTTTCACCCATTGCCTCTACGAGCAAATGGGTCTCATGCACGATGGTGTTTTTGTGGAACGCGATCTATT *F CGGGCTTCTTTCCGATGTCAGTAATACCGATTACTGGCCAGAACGTCAATGCCACGCGATTCGTGGCAATAACAAATGTG *F AGACGGCCTACAGGATTCATCAATGCCAACAGCAGTTGAAACAACAGCAACAGAACTTATTGGCCACCAAGGAGGTTGAG *F GTCACCACCACACCAGCTGGATCCGATGAAACAAAACC *F mnrvlvlllvlkmfalsesrfakininlgltvadespktiteemirlcgdqtdislrelnklqredfsdpsesvqcfthc *F lyeqmglmhdgvfverdlfgllsdvsntdywperqchairgnnkcetayrihqcqqqlkqqqqnllatkevevtttpags *F detkp *F Obp47b (CG13208) 47E5 *F ATGTCGCCCAGTCAGCTTCTTGTGATCTTCGCTTCGCTGGCCCTAAATACTCGTCTAGTATTTGGCCAAGCCACGATTGA *F TTGCCAAAGACCACCGCAACTAGTGGATCCCGCACTCTGCTGCAAGGATGGGGGTCGCGACCAGGTGGCTGAGCAGTGCG *F CCCAACGGATTTTAGGAACAGCAAATGGACAGAAGGCAGGAGGTCCTCCATCTTTGGACACGGCAGCATGCCTAGCTGAG *F TGCATCCTCACATCCTCCAAGTATATTGATGAGCCCCAGAAACTAAACCTGGCAAACATACGCAGTGATCTCTCCGCTAA *F GTTCTCCAACGATACCCTTTACGTGGAAACCATGACCATGGCGTTCTCCAAGTGTGAGCCCCAATCGCAGCGCAGACTGG *F CCATGATCATGCAGCAGCAACAACAGGTGCAGCAGCAAAAGACGCAGCAGCAACAACCAAGATGCAGCCCCTTCTCAGCC *F ATCGTCCTTGGGTGCACCTACATGGAGTACTTCAAGAACTGCCCCGATCATCGGTGGACTCCAAATGCCCAGTGTACTCT *F GGCCAAGGCCTACGTAACGCAGTGCGGTTTGGGTGCC *F mspsqllvifaslalntrlvfgqatidcqrppqlvdpalcckdggrdqvaeqcaqrilgtangqkaggppsldtaaclae *F ciltsskyidepqklnlanirsdlsakfsndtlyvetmtmafskcepqsqrrlamimqqqqqvqqqktqqqqprcspfsa *F ivlgctymeyfkncpdhrwtpnaqctlakayvtqcglga *F Obp49a (CG8769.m) 49B9 *F ATGCTTTCAAAATCGCAGCTATTACTGCTCGTTGTCGGGTTCTGCCTGAATGCAGCCGTCTCGGCTGACGTGGACTGCAG *F CAAGCGGCCATCGTTTGTCAATCCCAAAACCTGTTGTCCAATGCCGGACTTCGTGACCGCCGAGTTGAAGCAAAAGTGCA *F TCAAGTTTGACATGACTCCGCCGCCGCCGCCGGATGGGGAAGCAAGTGGATCCTTTGAGAGCAAGCGTCGTCATCATCAT *F CCGCATCCCCCACCATGCTTCTTCTCCTGCATCTTTAACGAGACTGGAATCTATCAAAACCGGAAACTGGATGAGGCAAA *F GCTGAACGCCTACTTGCAGGAGGTCTTCGAGGACAGTTCTGATCTGCAGACCACGGCCACCCAGGCCTTCACCACCTGTG *F CAACCAAAGTCGCGGATTTCGAGGCCAATTTACCTCCTCGTCCGGCTCCATCTCCGCCACCCGGATTCCCCATGTGTCCT *F CACGATGCTGGTCACCTCATGGGCTGTGTGTTCCGCAATATGATGAAGAATTGCCCAGACTCAATACGGAATGATTCCCA *F GCAGTGCACTGACATGAAAGAGTTCTTCACCAAGTGCAAGCCACCTCGTGGTCCTCCTCCTTCCGCCGAAGATATG *F mlsksqllllvvgfclnaavsadvdcskrpsfvnpktccpmpdfvtaelkqkcikfdmtpppppdgeasgsfeskrrhhh *F phpppcffscifnetgiyqnrkldeaklnaylqevfedssdlqttatqafttcatkvadfeanlpprpapspppgfpmcp *F hdaghlmgcvfrnmmkncpdsirndsqqctdmkefftkckpprgpppsaedm *F Obp50a 50F6 *F ATGCGGACAGGGCGTATACTTGTTGCTTTGATTTTTCTAGGTTTAATAATTCCCTTTCGGGCTGCAAAGTGCAGGGCAGC *F GCCCAAATCTGTGCAAAATGTACACGTTTGTTGCTCAGCACCCCTGCCTAACTGGGGAGTTTTCAACAGAGAGTGTCATA *F AATCGGCCATTCAAGCAAGTGTAAGTATTAATAGGATATCTAAAAGCAAAGTTAATTTAGCAAACTTCCTCATCAAGTGC *F CGTTTAGATTGCGATTTCAATGCCAGCTCGGTTCTGCAGGGAAATCGATTGATCCAAGCGAAAGTTCGACCCATGTTGGA *F GCGCGCCTTTTCCAACGAACCCACCATCGATGCATATGAGTCCAACTTTGCCAAATGTTCAACGGTGGTCAGGAGCAAAT *F ACCAGGAGCTATCTCCGCTGAGTCGTCAAAGCGACGCCTGTGACAGACACGCCCTCTTCTACAGCCTTTGTGCGTATGCT *F CGCTTGATTTTCACCTGTCCGGACAAAATGTGGCAAAGAAATAACAGGATGTGCCAGGAGGCCAAGGCCTATGCGAAAAA *F ATGTCCTTGGCCAGCGCTAAAAATGTTTATGAGGAATACC *F mrtgrilvaliflgliipfraakcraapksvqnvhvccsaplpnwgvfnrechksaiqasvsinriskskvnlanflikc *F rldcdfnassvlqgnrliqakvrpmlerafsneptidayesnfakcstvvrskyqelsplsrqsdacdrhalfyslcaya *F rliftcpdkmwqrnnrmcqeakayakkcpwpalkmfmrnt *F Obp50b (CG13940.m) 50F6 *F ATGTCTTCGGTGCTGCATCTACTGGGTTTCTTGTGGCTTCCTTTGCTGGTTTATAGTGTATCCAATGATATGGGAGGATT *F GCAGAAATGTACAGAACTTCTAAATACACATAAGTTGGTCTACTGTTGTGGCAAATCCTTTCTGGATAAGTTCCCCTTTG *F TTGGCAGCAATTGCACACCGTTTTGGGATGACTATGGTCCTTGCCGCTATGAATGTCTGTATAGGCACTGGGACCTCCTT *F GATCAGGATAACAAGATCAAAAAACCGGAGCTCTACCTGATGATCACCAGCCTCTACAGCCCCTTGAATGGTTATGATAA *F ATACGGGGCCGCTTTTAAGGCAGCTCACGAGACTTGCGAAGCTCTGGGCTCCAGACACGCCGACTTTCTGCTGCTCTACT *F CCAACCAGGTGGCGGATAAGATGGGCATGGCTTCCTCAACCTGTCTGCCATATGCCATGCTTCATGCACAGTGTACCATG *F GTATACCTAACCGCCAACTGTCCCCGTGAGAACTGGATAGATGATCCGAAGTGCAATAGTCTGCAAAAATTGCTGTCAAG *F TTGCACAAAGAAATTGGACGAAAAGACGAATGCACTTAAGGGAAAGGATGAGGAACTAACGGACAACGGGTGTGGGCATA *F TAGATTCAGAAGGATCCAATCTGCTCATGGCCTGTTTTCTCACACTGATGATCGCCAAGTTCATATCGGATCAT *F mssvlhllgflwlpllvysvsndmgglqkctellnthklvyccgksfldkfpfvgsnctpfwddygpcryeclyrhwdll *F dqdnkikkpelylmitslysplngydkygaafkaahetcealgsrhadflllysnqvadkmgmasstclpyamlhaqctm *F vyltancprenwiddpkcnslqkllssctkkldektnalkgkdeeltdngcghidsegsnllmacfltlmiakfisdh *F Obp50c 50F6 *F ATGGCCCGGCATATAGCCCTGCTGATCTGCTCATTGCTAGCGATGGCTGGCTGTGATCCAATCGATGTGGACTGTACTCG *F CAGACAGGATTTCAATATTGTCAAGGACTGCTGTGTCTATCCCACATTTAGGTTTGACCAGTTCAAAAGCCAGTGTGGTA *F AATATATGCCAGTTGGTGCTCCCAGAATTTCACCCTGCCTCTATGAATGCATTTTCAATAAGACCAACACAGTTGTGGAC *F GGAGCTATTCATCCTGACAATGCCCGACTCATGCTGGAGAAGCTTTTCGGTAATCAGGACTTTGAAGAAGCCTATTTCAA *F TGGTTTAATGGGCTGTTCGGATTCTGTGCAAGAGATGATTAGCAACAGGAGGTCACGGCCCCAAAGAAAAACAGAACAAT *F GCTCTCCATTCTCACTTTTCTATGGAATTTGTGCCCAGAGATATGTCTTCAACCATTGTCCATCATCCAGCTGGTCCGGC *F ACTGAATCTTGCGAAATGGCCCGATTGCAGAACATGAACTGTTCGAAACCATCACGTGGTTCTAGTCATCGCCTT *F marhiallicsllamagcdpidvdctrrqdfnivkdccvyptfrfdqfksqcgkympvgaprispclyecifnktntvvd *F gaihpdnarlmleklfgnqdfeeayfnglmgcsdsvqemisnrrsrpqrkteqcspfslfygicaqryvfnhcpssswsg *F tescemarlqnmncskpsrgsshrl *F Obp50d 50F6 *F ATGCTTCACAAGCTGACTTGGGTTCTAATATTTATACCTGCTTTTCGAGCTGCCGATCCCATTTGCTCTCAAAGGCCGGA *F TGTAACTGCCTTGAGAAACTGTTGCAAGCTGCCTAATCTGGACTTTTCGAGTTTCAACTCCAAGTGCAGTCAGTATTTGG *F TCAATGGAGTCCACATATCACCTTGCAGCTTTGAGTGCATCTTTCGAGCGGCGAATGCATTAAATGGCACCCATTTGGTT *F ATGGAAAATATCGAAAAGATGATGAAAACCATTTTGGGCTCTGATGAATTTGTGCACGTGTATCTGGATGGATTCAGGAG *F CTGCGGTAATCAGGAGAAGGTGCTCATTAAGGCAATGAAACGTCGCCGTGTTCCCATCACCGGAAAATGTGGCTCGATGG *F CGATAATGTATGGCCTGTGTGCCCATCGATATGTCTACCGTAATTGCCCGGAAAGCGTGTGGAGCAAGAGTGCCACCTGC *F AATGAGGCCAGGGAATACAGCATTCGCTGCGATGACATG *F mlhkltwvlifipafraadpicsqrpdvtalrnccklpnldfssfnskcsqylvngvhispcsfecifraanalngthlv *F meniekmmktilgsdefvhvyldgfrscgnqekvlikamkrrrvpitgkcgsmaimyglcahryvyrncpesvwsksatc *F neareysircddm *F Obp50e (CG13939.m) 50F6 *F ATGCATAAATATATAATTTGTTTTGGTTTTTTACTAATTATTCTGGAATGTTCGTTGGCGTCATTTAACTGCTCAGCACC *F GCCGAATTTCAATAACTTTGATATCAACACTTGCTGTCGCACTCCGGAATTGGATATGGGTGATGTGCCCCAGAAGTGCC *F ACAAATATGTGAGTGGCTTAAAGTCGGCGAACTCCAAGTATCCCAGCTATGCTCATCTGTGCTATCCTGATTGCATTTAT *F CGCGAGACGGGTGCCATGGTCAATGGAAAGATTAAAGTGAACAGAGTTAAGCAGTACTTGGAAGAGCACGTTCATCGGCG *F GGATCAGGAAATTGTTTCTCACATAGTGCAATCCTTCGAATCCTGTCTGTCGAACGTTAAAGGTCACATGAAGTCATTGA *F ACATTGAGTCGTACAAAGTGCTGCCCCATGGCTGTTCCCCCTTTGCCGGAATCATATACAGCTGTGTGAATGCCGAGACT *F TTTCTCAATTGCCCCCAGCAAATGTGGAAAAACGAGAAACCCTGTAATTTGGCCAAGCAATTCGCCGAGCAGTGCAATCC *F ACTGCCTCATGTTCCGTTGCCCAGCAGC *F mhkyiicfgflliilecslasfncsappnfnnfdintccrtpeldmgdvpqkchkyvsglksanskypsyahlcypdciy *F retgamvngkikvnrvkqyleehvhrrdqeivshivqsfesclsnvkghmkslniesykvlphgcspfagiiyscvnaet *F flncpqqmwknekpcnlakqfaeqcnplphvplpss *F Obp51a (OBP51a) 51D10-11 *F ATGAAAGTATTCATTGGCCTGGTTCTGTTGTTAGCTGTCACTACGCTGTCATCCGCTTTATTCGAATCTGAAGCGAACGA *F ATGTGCTAAAAAACTGGGAATTACCCCAGATTACTTCGAAAATTTTCCGCACAGCAGTCGGGTGAAGTGCTTTTACCACT *F GCCAAATGGAAAAACTTGAAATAATTGCCAATGGTGTGGTAACACCATTCGATTTGAAAGTATTGAACATATCACCGGAG *F AGCTATGATAAGTATGGTGTAAAGGTAAAACCATGCCTCAAACTATCGCATCGCGACAAATGTGAGCTCGGTTACTTGGT *F GTTCCAGTGCTTGAAACGAGAATTTAACTTG *F mkvfiglvlllavttlssalfeseanecakklgitpdyfenfphssrvkcfyhcqmekleiiangvvtpfdlkvlnispe *F sydkygvkvkpclklshrdkcelgylvfqclkrefnl *F Obp56a (CG11797, clot \#14015, OBP56a) 56E4 *F ATGAACTCCTACTTCGTGATCGCTTTGAGTGCTCTTTTTGTGACTCTGGCTGTTGGATCGTCCCTTAATCTGAGCGACGA *F GCAGAAGGATCTGGCCAAGCAGCATCGTGAGCAGTGCGCCGAGGAAGTGAAACTTACCGAGGAGGAAAAGGCCAAGGTCA *F ATGCCAAGGACTTCAATAACCCCACCGAGAACATCAAGTGCTTTGCCAACTGCTTCTTCGAGAAGGTCGGAACCCTGAAG *F GACGGTGAGCTGCAGGAGTCAGTGGTGCTGGAGAAGCTCGGCGCTCTCATTGGCGAGGAGAAGACCAAGGCGGCTCTGGA *F GAAGTGCAGGACCATCAAGGGCGAGAACAAGTGTGATACCGCCTCCAAGTTGTACGATTGCTTCGAGAGCTTCAAGCCCG *F CCCCCGAGGCTAAGGCC *F mnsyfvialsalfvtlavgsslnlsdeqkdlakqhreqcaeevklteeekakvnakdfnnptenikcfancffekvgtlk *F dgelqesvvleklgaligeektkaalekcrtikgenkcdtasklydcfesfkpapeaka *F Obp56b (CG15129.m2, OBP56b) 56E4 *F ATGAAACTTATCTACTTGTTGGTTGTATTCCTAATTTTCGCTCTAAGCGAACTAGTAGCG *F GGCCAGTCAGCTGCGGAATTGGCAGCCTACAAGCAAATTCAACAGGCCTGCATCAAGGAGCTGAATATTGCTGCCAGTGA *F TGCTAATTTGCTGACCACCGACAAGGAGGTGGCGAATCCCTCTGAGTCGGTGAAGTGCTATCACAGCTGCGTCTACAAGA *F AACTGGGTCTCCTGGGTGACGATGGAAAGCCCAATACTGATAAGATCGTTAAGTTGGCCCAGATCCGTTTCAGCAGTCTG *F CCGGTGGATAAGCTAAAGAGTTTGCTTACCAGCTGCGGAACCACAAAGTCAGCCGCCACCTGTGACTTTGTCTACAACTA *F TGAAAAGTGTGTTGTTAAGGGTATTAGTGCC *F mkliyllvvflifalselvagqsaaelaaykqiqqacikelniaasdanllttdkevanpsesvkcyhscvykklgllgd *F dgkpntdkivklaqirfsslpvdklkslltscgttksaatcdfvynyekcvvkgisa *F Obp56c (CG15129.m1, OBP56c.m) 56E4 *F ATGTATTTTCGAGCCAGTTTGATGGCATTGCTTTGCCTCACTCTTAGTGAATTCGTTTCTAAAGCATGGGTAATGTTTTT *F CATATTTTATATATCTTTTACCCGATCGCTTTCCGTCTCGCTGAACATGTCGATGACACGAACCCTGGTTCCAGATCCGC *F CAAATGGAACAGAAAACAAACTCAGCCAGGAGATGCTGAGGGCTTGTATGCGTAGGACCGAGATCTCAATGTCGCAACTG *F AAACTCTTTCACATGAGCCTGATGAACAGCGACTACAATAATGACAACGATATAGCCCCTACGCCAGTTCAATCCATTGG *F CGATGTAAATAACCTGGGTGATCTGGACTTCAATGGCAACTCGCAGATGCCATATCTCGATCTGAAGCATAATGAGCCGC *F TGCAGTGCTTTGTGAGCTGTCTGTATGAGACCCTGGATTTGGATAGGTACAATGTCCTGCTGGAGGAGGCCTTTAAGAAT *F CAGGTGCAAACGATCATACAGCATGAGAAGGCGGAGATCAAGGAGTGTAGTGATCTTCAGGGCAAAACACGATGCGAGGC *F AGCCTACAAGCTGCACCTGTGCTACAATCACCTGAAAACTCTGGAGGCGGAGCAGCGTATCCGTGAGATACTTGAGCGGA *F CCGAGGCGGAGAACGAGGGATTCGGTCCGGAGGGCAGCGACTTTATCGACGGCATCCAGCATTCCGGAGAAGCAATGACC *F ACCGCTAAGTCGGAG *F myfraslmallcltlsefvskawvmffifyisftrslsvslnmsmtrtlvpdppngtenklsqemlracmrrteismsql *F klfhmslmnsdynndndiaptpvqsigdvnnlgdldfngnsqmpyldlkhneplqcfvsclyetldldrynvlleeafkn *F qvqtiiqhekaeikecsdlqgktrceaayklhlcynhlktleaeqrireilerteaenegfgpegsdfidgiqhsgeamt *F takse *F Obp56d (CG11218.m, clot \#1175, OBP56d.m) 56E4 *F ATGAAGTTCCTGATTGTCCTCTCCGTCATTTTGGCCATTTCGGCTGCTGAGCTGCAACTATCCGATGAGCAGAAGGCTGT *F GGCCCATGCCAATGGCGCCCTTTGTGCCCAGCAGGAGGGAATCACCAAGGATCAGGCGATCGCCTTGCGAAACGGCAATT *F TCGATGACAGTGACCCCAAGGTGAAATGCTTCGCCAATTGTTTCTTGGAGAAAATCGGATTCCTGATCAATGGTGAGGTC *F CAGCCCGATGTCGTTCTGGCCAAGTTGGGACCCCTGGCCGGCGAGGATGCCGTGAAGGCCGTTCAGGCCAAGTGTGATGC *F CACCAAGGGAGCGGATAAGTGCGATACTGCCTATCAGCTATTCGAGTGCTACTACAAGAATCGCGCCCACATC *F mkflivlsvilaisaaelqlsdeqkavahangalcaqqegitkdqaialrngnfddsdpkvkcfancflekigflingev *F qpdvvlaklgplagedavkavqakcdatkgadkcdtayqlfecyyknrahi *F Obp56e (CG8462, clot \#16275, OBP56e) 56E5 *F ATGAAAGTATTCTTTGTGTTTGCCGCCCTTGCAGCTCTATCTTTGGCATCTGCCGTGGGGCTAACTGATTCCCAAAAGGC *F TGAGGCAAAGCAGAGAGCCAAGGCCTGCGTCAAACAGGAGGGAATCACGAAGGAGCAAGCTATTGCCCTGCGGTCTGGAA *F ACTTTGCAGACTCCGATCCAAAGGTAAAGTGCTTCGCCAACTGCTTCCTGGAGCAGACCGGCCTGGTGGCCAATGGGCAG *F ATAAAACCTGACGTGGTTTTGGCCAAACTAGGTCCCATCGCCGGCGAAGCCAATGTCAAGGAGGTGCAGGCCAAGTGTGA *F CTCGACCAAGGGAGCCGACAAGTGCGACACTAGCTATCTGCTGTACAAGTGCTACTACGAAAACCACGCCCAATTC *F mkvffvfaalaalslasavgltdsqkaeakqrakacvkqegitkeqaialrsgnfadsdpkvkcfancfleqtglvangq *F ikpdvvlaklgpiageanvkevqakcdstkgadkcdtsyllykcyyenhaqf *F Obp56f (OBP56f) 56E5 *F ATGAAAGTATTCCTGTTGTTCATTTTCATCTCTGCTATCTGGCTCCAAGCATTTTGTATGAAATCTTCTGAAAAAATAAA *F AGCCTGCTTGAAACGGCAGCTGGGGTATACAATTACAGAAAATACAAAATTTGATGCTAAAGAAGACTCTCTTCAAAGCA *F AGTGTTTTTATCACTGCTTACTGGAAGTGAAAGGTGTTATTGCAAATGATGCGATCAGTTCGGAGCAACCGAGGAAAGTA *F CTTGAAAAAAAGTATGGCATTACTGACACAGATGAATTGGAAAAGGCTGAAGAAAAGTGTCATTCCATCAAGGCTTCAGG *F AAAATGTGAATTGGGCTACGAAATCTTGAAATGCTATCAGTCCATTACCAAGCAT *F mkvfllfifisaiwlqafcmkssekikaclkrqlgytitentkfdakedslqskcfyhcllevkgviandaisseqprkv *F lekkygitdtdelekaeekchsikasgkcelgyeilkcyqsitkh *F Obp56g (CG13873.m, OBP56g) 56F1 *F ATGAGGGCTACATTCGCATTGACTCTGTTGCTCGGCTGCCTTTCAGGAATTTTGGCGCAGCAAGCCAACATAGACAGTTC *F GGTGTCCAAGGAACTGGTGACGGATTGCCTCAAGGAGAACGGTGTCACTCCCCAGGATCTGGCTGACTTGCAATCGGGCA *F AGGTGAAGGCCGAGGATGCCAAGGACAATGTGAAGTGCTCCTCACAGTGCATTCTGGTCAAGAGCGGTTTCATGGACTCC *F ACTGGCAAACTGCTGACCGACAAGATTAAGTCTTACTATGCGAACTCGAACTTTAAGGATGTCATCGAAAAGGATTTGGA *F CAGGTGCAGCGCGGTCAAGGGGGCCAATGCCTGTGACACTGCCTTCAAGATATTATCCTGCTTCCAGGCAGCCAAT *F mratfaltlllgclsgilaqqanidssvskelvtdclkengvtpqdladlqsgkvkaedakdnvkcssqcilvksgfmds *F tgklltdkiksyyansnfkdviekdldrcsavkganacdtafkilscfqaan *F Obp56h (CG13874.m, OBP56h) 56F2 *F ATGAAGTTCACCCTATTCTGTATTGCTCTGGCAGCTTTTTTGTCCATGGGACAGTGTAATCCGGACTTTCGCCAAATAAT *F GCAACAGTGCATGGAGACCAACCAAGTGACCGAGGCTGATCTCAAGGAGTTCATGGCCAGCGGGATGCAGAGCAGTGCCA *F AGGAGAACCTCAAGTGCTACACCAAGTGCCTGATGGAGAAGCAGGGTCATCTCACCAATGGCCAGTTCAATGCTCAGGCT *F ATGCTCGACACTCTCAAAAATGTGCCTCAGATCAAGGACAAAATGGACGAGATTTCCTCGGGAGTGAATGCCTGCAAGGA *F CATCAAGGGAACCAACGATTGCGACACGGCCTTTAAGGTTACCATGTGCCTGAAGGAGCACAAGGCCATTCCAGGACATC *F AC *F mkftlfcialaaflsmgqcnpdfrqimqqcmetnqvteadlkefmasgmqssakenlkcytkclmekqghltngqfnaqa *F mldtlknvpqikdkmdeissgvnackdikgtndcdtafkvtmclkehkaipghh *F Obp56i (OBP56i) 56F2 *F ATGCATTTTTTCACCTGCTGTGCATTATTGTTAGTCGTCGTTACATTACCAACATGTTTCGTACAAGCAGGTCCCATTAA *F GGATCAATGCATGGCGGCGGCGGGCATCACAGCACAAGATGTTGCGAATCGTCATGAGACCGACGACCCTGGCCATAGTG *F TCAAGTGCTTTTTCCGCTGTTTTCTAGAAAACATTGGCATTATCGCCGATAACCAGATAATACCCGGTGCTTTTGACCGA *F GTTCTAGGCCATATAGTTACCGCGGAAGCCGTAGAGCGAATGGAAGCGACGTGTAATATGATTAAGAGCGAGACATCCCA *F TGACGAGTCTTGTGAATTCGCCTGGCAAATCTCCGAGTGCTACGAAGGAGTAAGATTATCAGATGTTAAGAAGGGCCAAA *F GAACTCGAAATCACAGAGGA *F mhfftccalllvvvtlptcfvqagpikdqcmaaagitaqdvanrhetddpghsvkcffrcflenigiiadnqiipgafdr *F vlghivtaeavermeatcnmiksetshdescefawqisecyegvrlsdvkkgqrtrnhrg *F Obp57a (CG13421, GH01026, OBP57c) 57A6 *F ATGCTTAAGCTATGGCTAATATGTATCTTGACTGTCAGCGTGGTCTCCATACAGAGTCTCTCACTTCTGGAGGAAACCAA *F TTACGTGTCAGATTGCCTGGCCAGCAATAACATCAGCCAGGCTGAATTCCAGGAACTCATCGATCGCAACAGCTCCGAAG *F AGGATGACCTCGAGAATACCGACAGAAGATACAAATGCTTCATCCATTGCCTAGCGGAGAAGGGAAATCTTTTGGATACC *F AATGGCTATTTGGATGTGGATAAAATTGATCAAATTGAGCCCGTGAGCGATGAACTGCGAGAGATACTCTACGATTGCAA *F GAAAATCTACGATGAGGAAGAAGATCATTGCGAATATGCGTTTAAGATGGTGACTTGTCTAACTGAAAGCTTCGAGCAGA *F GCGATGAGGTCACCGAAGCGGGTAAAAATACAAACAAATTAAACGAA *F mlklwliciltvsvvsiqslslleetnyvsdclasnnisqaefqelidrnsseeddlentdrrykcfihclaekgnlldt *F ngyldvdkidqiepvsdelreilydckkiydeeedhceyafkmvtcltesfeqsdevteagkntnklne *F Obp57b (OBP57b) 57A6 *F ATGTTCATCTACAGACTTGTATTTATTGCGCCTCTGATTTTGTTATTGTTCAGCTTGGCCAAGGCTCGCCACCCCTTTGA *F TATATTTCATTGGAATTGGCAAGACTTTCAGGAGTGTCTACAAGTTAATAATATTACCATAGGAGAATATGAGAAATACG *F CGCGACACGAAACTTTGGATTACCTGCTCAACGAGAAAGTCGACTTGAGGTACAAGTGCAATATTAAATGTCAGCTGGAA *F AGGGATTCAACGAAATGGTTGAATGCTCAAGGCAGAATGGATTTGGATTTGATGAATACGACCGATAAGGCATCCAAATC *F CATTACCAAGTGCATGGAGAAGGCTCCCGAAGAACTTTGTGCGTACAGTTTTAGACTGGTGATGTGTGCATTTAAGGCTG *F GCCATCCCGTAATTGATTCGGAA *F mfiyrlvfiaplilllfslakarhpfdifhwnwqdfqeclqvnnitigeyekyarhetldyllnekvdlrykcnikcqle *F rdstkwlnaqgrmdldlmnttdkasksitkcmekapeelcaysfrlvmcafkaghpvidse *F Obp57c (OBP57a) 57A6-7 *F ATGTTCAACACTAGACTTGCCATTTTTTTGCTTCTTATCGTTGTTTCGCTTAGCCAAGCTAAGGAAAGCCAACCCTTTGA *F CTTTTTCGAAGGAACCTATGACGATTTTATTGATTGTCTGAGAATCAATAATATTACCATTGAAGAGTATGAGAAGTTTG *F ACGATACCGACAATTTGGATAATGTCCTCAAGGAAAATGTCGAACTGAAGCACAAGTGCAACATTAAGTGTCAACTGGAA *F AGAGAGCCAACCAAATGGCTAAATGCTCGGGGTGAAGTCGATCTGAAATCAATGAAAGCAACCAGTGAGACAGCGGTATC *F CATATCAAAGTGCATGGAGAAGGCTCCCCAAGAAACCTGTGCCTACGTCTATAAATTGGTAATATGTGCATTCAAATCCG *F GACATTCAGTCATCAAGTTCGATTCATATGAACAAATACAAGAGGAAACCGCTGGACTAATAGCTGAACAGCAGGCGGAT *F CTGTTTGATTACGATACCATCGATTTA *F mfntrlaiflllivvslsqakesqpfdffegtyddfidclrinnitieeyekfddtdnldnvlkenvelkhkcnikcqle *F reptkwlnargevdlksmkatsetavsiskcmekapqetcayvyklvicafksghsvikfdsyeqiqeetagliaeqqad *F lfdydtidl *F Obp57d (CG13429.m, OBP57e) 57A8 *F ATGTTGGACCAACTTACACTGTGTTTGTTGCTAAATTTTCTGTGCGCAAATGTTCTCGCTAACACTTCAGTATTTAATCC *F GTGTGTTTCGCAAAATGAGTTATCCGAATATGAAGCCCACCAAGTGATGGAGAATTGGCCAGTTCCGCCCATCGATCGGG *F CTTACAAATGCTTTCTAACATGCGTCCTCTTGGATTTGGGTCTGATTGATGAACGGGGTAATGTGCAGATCGATAAGTAC *F ATGAAATCCGGAGTGGTGGACTGGCAATGGGTGGCAATAGAGTTGGTAACATGTCGCATAGAATTCAGCGACGAAAGGGA *F TCTGTGCGAGCTATCATATGGAATCTTCAACTGCTTCAAGGATGTGAAGCTTGCGGCCGAGAAGTATGTTTCAATTAGTA *F ATGCAAAG *F mldqltlclllnflcanvlantsvfnpcvsqnelseyeahqvmenwpvppidraykcfltcvlldlglidergnvqidky *F mksgvvdwqwvaielvtcriefsderdlcelsygifncfkdvklaaekyvsisnak *F Obp57e (OBP57d) 57A8 *F ATGTCTCTAAGACTTGTACCGCATCTGGCTTGTATTATTTTTATTTTGGAAATTCAATTTAGAATTGCCGATTCTAACGA *F TCCGTGCCCCCATAATCAAGGAATAGACGAAGATATAGCCGAATCAATTCTAGGTGACTGGCCTGCAAATGTGGATTTGA *F CTAGCGTGAAAAGGTCCCACAAGTGTTATGTGACCTGTATTTTGCAATATTACAATATTGTGACCGCTTCTGGTGAGATA *F TTTCTGGACAAGTACTACGATACTGGAGTCATTGATGAATTGGCGGTGGCACCCAAAATCAATCGATGCCGATATGAGTT *F TAGAATGGAAACAGATTATTGTAGCCGAATTTTTGCTATATTCAATTGTTTAAGGCAAGAAATATTAACAAAGTCA *F mslrlvphlaciifileiqfriadsndpcphnqgidediaesilgdwpanvdltsvkrshkcyvtcilqyynivtasgei *F fldkyydtgvidelavapkinrcryefrmetdycsrifaifnclrqeiltks *F Obp58a (CG13517) 58F6 *F ATGAAACAGTTGATTTTCCTGCTGATTTGTTTGAGCTGCGGCACCTGCTCCATTTACGCACTGAAATGCAGATCCCAGGA *F GGGACTAAGTGAAGCGGAGCTCAAGCGAACTGTGCGCAACTGTATGCATCGCCAGGACGAGGACGAAGATCGAGGACGAG *F GTGGACAGGGCCGGCAAGGAAATGGCTATGAGTACGGTTACGGAATGGATCACGATCAGGAGGAGCAGGACAGGAATCCA *F GGCAACAGGGGCGGCTATGGCAATCGAAGGCAGCGAGGACTAAGGCAATCGGATGGCAGGAACCACACCAGCAACGATGG *F AGGTCAGTGTGTGGCCCAGTGCTTTTTCGAGGAGATGAATATGGTGGATGGCAATGGGATGCCCGATCGGCGCAAGGTGA *F GCTATTTGCTGACCAAGGACCTTCGGGACCGGGAGCTGCGCAACTTCTTCACGGACACCGTGCAGCAGTGCTTCCGCTAT *F CTGGAGAGCAACGGAAGGGGCCGGCACCACAAGTGTTCAGCGGCCCGGGAACTGGTCAAGTGCATGTCGGAGTACGCGAA *F GGCGCAGTGCGAGGATTGGGAGGAGCACGGCAACATGCTCTTCAAT *F mkqliflliclscgtcsiyalkcrsqeglseaelkrtvrncmhrqdededrgrggqgrqgngyeygygmdhdqeeqdrnp *F gnrggygnrrqrglrqsdgrnhtsndggqcvaqcffeemnmvdgngmpdrrkvsylltkdlrdrelrnfftdtvqqcfry *F lesngrgrhhkcsaarelvkcmseyakaqcedweehgnmlfn *F Obp58b (CG13518.m) 58F6 *F ATGCTGAGAATTGGTTTCGTGATTTGTGTGATTATATCACTGCGCTTGAATGGACTCGTGGCTGTCCGAGTTCATTGCCG *F GCACATGGAGCGTATTCACGAGGAGAACATCCACCATTGCTGCAAACATCAGGATGGCCACGACGATGTCACGGAATCTT *F GCGCCAAGCAGACCAACTTTCGTTTGCCCAGTCCCAATGAGGAGGCCATCGTGGATGTGACCGTGGACCAGGCGATGGTT *F GGAACCTGCTGGGCCAAGTGCGTCTTCGATCACTACAATCTGATGGAGAACAACACACTGGATATGGACAAGGTGCGCTC *F GTACTACAAGCGGTACCATCAAACAGATCCGGAGTACGCGACTGAAATGCTGAATGCCTACGAAAAATGTCACACACAAT *F CTGAAGAGGCCACCGAAAAGTTCCTATCACTTCCAATTGTTAGGGCCTTCTCCACCGCCAAGTTCTGTAAGCCGACCTCG *F AGTATCATCATGTCCTGCGTCATCTACAACTTCTTCCACAATTGTCCAGCGAGTCGCTGGTCAAATACCACCGAGTGCGT *F GGAAACCTTGGCCTTTGCTAGGAAGTGCAAGGATGTGCTGACTACTATG *F mlrigfvicviislrlnglvavrvhcrhmeriheenihhcckhqdghddvtescakqtnfrlpspneeaivdvtvdqamv *F gtcwakcvfdhynlmenntldmdkvrsyykryhqtdpeyatemlnayekchtqseeatekflslpivrafstakfckpts *F siimscviynffhncpasrwsnttecvetlafarkckdvltt *F Obp58c (CG13524) 58F6 *F ATGAAGTGCACCATTTTATTGAGTTTTTTCAGCCTGATTTGGTTTGCTGGTGGAATTAAAATTGATTGCGAGAATACGGA *F GGCCATTAACGAGGACCACATCCACTATTGCTGCAAGCATCCCGATGGACACAATGATCTAATCGAAGGATGTGCCAGGG *F AGACCAATTTTACGCTGCCCAATCAAAACGAGGAGGCACTGGTGGACATCACGGCGGACCGAGCCATCCGAGGCACTTGT *F TTTGGCAAATGCGTCTTTAGCAAACTGAACCTGATGAAGGACAACAACCTGGACATGGATGCCGTGAGAAGTTTGTTCAC *F TGAAAGGTTTCCCGACGATCCGGAATATGCCAAGGAAATGATCAACGCCTTTGATCATTGTCACGGCAAAAGCGAAGAGA *F ATACCTCCATGTTCCTGTCGAAGCCCCTCTTCAAGCAAATGTCCAAGCAATTCTGCGATCCGAAGTCTAGTGTCGTCCTG *F GCCTGTGTCATCCGCCAGTTCTTCCACAACTGCCCGGCGGATCGCTGGTCGAAGACCAAGGAGTGCGAGGATACCCTGGC *F CTTCAGCAAGAAGTGCCAGGACTCGCTGGCTACTTTG *F mkctillsffsliwfaggikidcenteainedhihycckhpdghndliegcaretnftlpnqneealvditadrairgtc *F fgkcvfsklnlmkdnnldmdavrslfterfpddpeyakeminafdhchgkseentsmflskplfkqmskqfcdpkssvvl *F acvirqffhncpadrwsktkecedtlafskkcqdslatl *F Obp58d (CG13519) 58F6-7 *F ATGGTCAACATTGTTTGTTATTGGACGTTCCTAATTCTGGTGGCCGTTTCAAAGGCTCAGGATAATGAGGAAACTACTGC *F AGTTGCCATATCTTCAGGTGACTTGACTGAGGATAAGTGTAACACATCGAGGGCTGGCTGCTGTTCTGAACTTTATATAG *F GAGAAGAGGAGGACCTGGTCAAGTGCTTCGTTATACACTCACCCAAGTTGCCAGTGGATGGGGACGCTGACATCGGCAAG *F ACACTGAGGTTTCTATCGTGCTTTGTGGAGTGCCTGTACAAGCAAAAGAAGTACATTGGTAAGAGCGACACCATCAACAT *F GAAGATGGTCAAGCTGGATGCAGAAAAGACATTTGTGGATCGGCCCAAGGAGAAGGATTATCACATTGCCATGTTCGAGT *F TCTGTCGCAAGGATGCTGTTGGGGTCTACAACCTTTTAAAGGCCAGTCCTGGGGCTAAGGTGCTCCTGAAAGGAGCCTGT *F CGCCCCTATCTATTGATGGTATTCATGTGCATCAGTGACTATCATCAGAAGCACGAGTGTCCCTACTTCCGGTGGGAGGG *F CACTGCCAAGGCAGGAACTAAGGATATGTGCGAAAATGCCAAAGCCGAATGCTACCAAATAGATGGAATTACACTGCCCA *F CAAAGAGTCCTGCC *F mvnivcywtflilvavskaqdneettavaissgdltedkcntsragccselyigeeedlvkcfvihspklpvdgdadigk *F tlrflscfveclykqkkyigksdtinmkmvkldaektfvdrpkekdyhiamfefcrkdavgvynllkaspgakvllkgac *F rpyllmvfmcisdyhqkhecpyfrwegtakagtkdmcenakaecyqidgitlptkspa *F Obp69a (PBPRP-1, OBP69a) 69B2 *F ATGGTTGCAAGGCATTTTAGTTTTTTTTTAGCACTACTCATACTATATGATTTAATTCCTAGTAATCAAGGAGTGGAAAT *F TAATCCTACGATCATAAAGCAGGTGAGAAAACTGCGAATGCGATGCTTAAATCAGACAGGAGCTTCTGTAGATGTGATTG *F ACAAGTCGGTGAAAAATAGAATACTACCCACAGATCCCGAGATCAAGTGTTTTCTCTACTGCATGTTTGATATGTTCGGA *F TTGATTGATTCACAAAACATAATGCACTTGGAAGCACTGTTGGAGGTTTTACCCGAGGAAATATACAAAACGATTAACGG *F ATTAGTCAGTTCATGTGGAACTCAGAAGGGAAAAGATGGCTGTGATACCGCTTATGAAACCGTCAAGTGCTACATTGCTG *F TAAACGGAAAATTCATATGGGAAGAGATAATAGTGCTACTTGGG *F mvarhfsfflallilydlipsnqgveinptiikqvrklrmrclnqtgasvdvidksvknrilptdpeikcflycmfdmfg *F lidsqnimhleallevlpeeiyktinglvsscgtqkgkdgcdtayetvkcyiavngkfiweeiivllg *F Obp76a (LUSH, OBP76c) 76B9-C1 *F ATGAAGCATTGGAAACGACGCTCTTCCGCTGTTTTCGCGATCGTCCTGCAAGTGCTGGTACTCCTGCTACCCGATCCTGC *F AGTTGCCATGACGATGGAGCAGTTCTTGACCTCGCTAGACATGATCCGCAGTGGCTGTGCGCCGAAGTTTAAGCTCAAAA *F CAGAAGATCTCGATCGGCTTCGCGTGGGTGATTTCAACTTTCCGCCATCGCAGGATCTTATGTGCTACACAAAGTGTGTG *F TCTTTGATGGCGGGCACTGTGAACAAAAAGGGGGAGTTCAACGCTCCCAAGGCGCTGGCCCAACTTCCGCATCTGGTTCC *F CCCGGAAATGATGGAGATGTCCAGGAAATCCGTTGAAGCTTGTCGGGATACGCATAAACAATTTAAGGAATCTTGCGAGA *F GGGTCTACCAGACGGCCAAGTGCTTCTCTGAAAACGCCGATGGGCAGTTCATGTGGCCT *F mkhwkrrssavfaivlqvlvlllpdpavamtmeqfltsldmirsgcapkfklktedldrlrvgdfnfppsqdlmcytkcv *F slmagtvnkkgefnapkalaqlphlvppemmemsrksveacrdthkqfkescervyqtakcfsenadgqfmwp *F Obp83a (OS-F, PBPRP-3, OBP83a) 83C8-D1 *F ATGGACCAGGAAGGACCACGCAGCAGCGGAAAGGAGCGAAACGGAAAGAGCCACATTAAAATGGCTTTGAATGGCTTTGG *F TCGGCGTGTCAGTGCGTCTGTCCTTTTAATCGCCTTGTCGCTGCTCAGCGGAGCGCTGATCCTGCCGCCGGCTGCGGCGC *F AGCGTGACGAGAACTATCCACCGCCGGGCATCCTGAAAATGGCCAAGCCCTTCCACGACGCGTGTGTGGAGAAGACGGGC *F GTAACCGAGGCTGCCATCAAGGAGTTCAGCGATGGGGAGATTCACGAGGACGAGAAGCTCAAATGCTACATGAACTGCTT *F CTTCCACGAGATCGAAGTGGTGGACGACAATGGGGACGTGCATCTGGAGAAGCTCTTCGCCACGGTACCGCTCTCCATGC *F GCGACAAGCTGATGGAGATGTCCAAGGGCTGCGTCCATCCGGAGGGCGATACGCTGTGCCACAAGGCCTGGTGGTTCCAC *F CAGTGCTGGAAAAAGGCCGATCCCAAGCACTACTTCTTGCCG *F malngfgrrvsasvllialsllsgalilppaaaqrdenypppgilkmakpfhdacvektgvteaaikefsdgeihedekl *F kcymncffheievvddngdvhleklfatvplsmrdklmemskgcvhpegdtlchkawwfhqcwkkadpkhyflp *F Obp83b (OS-E, OBP83b) 83D1 *F ATGGTCAAATACCCACTGATACTACTTTTGATTGGCTGTGCCGCTGCCCAGGAACCAAGGCGCGATGGAGAGTGGCCTCC *F GCCAGCGATTTTAAAACTGGGCAAGCACTTCCATGACATTTGTGCTCCCAAAACTGGCGTTACTGATGAGGCCATCAAGG *F AGTTCAGCGATGGGCAAATTCATGAGGACGAGGCCCTCAAGTGCTATATGAACTGCCTCTTCCACGAGTTCGAGGTGGTC *F GACGACAATGGGGATGTCCACATGGAGAAGGTCTTGAACGCCATTCCGGGAGAAAAGCTGAGGAACATTATGATGGAGGC *F TTCCAAGGGATGCATTCATCCTGAGGGCGACACCCTGTGCCACAAAGCCTGGTGGTTCCACCAATGCTGGAAGAAGGCTG *F ATCCTGTCCACTACTTTTTGGTC *F mvkyplillligcaaaqeprrdgewpppailklgkhfhdicapktgvtdeaikefsdgqihedealkcymnclfhefevv *F ddngdvhmekvlnaipgeklrnimmeaskgcihpegdtlchkawwfhqcwkkadpvhyflv *F Obp83c (CG15582.m1, OBP83c) 83D4 *F ATGCAGATGAAAAGTGGAATATTAATAGCATTATGTCTATGCCTTTCGTTGAACGAAGGCCTGGCCCTTCTGGAGCACGA *F AGGCGAGACCATCAACAGATGCATCCAAAACTATGGCGGACTTACTGCGGAAAATGCCGAACGTCTAGAACGATTCAAGG *F AATGGTCGGATAGCTACGAGGAAATCCCCTGCTTCACGCGCTGCTATTTGTCCGAGATGTTCGACTTTTACAATAACTTA *F ACGGGCTTCAATAAGGACGGAATTGTGGGCGTCTTTGGAAGACCCGTCTACGAAGCCTGCCGAAAGAAATTGGAACTGCC *F ATTCGAATCAGGCGAGAGCAGCTGCAAACATGCCTACGAGGGCTTCCACTGCATCACCAACAAAGAATTC *F mqmksgilialclclslneglallehegetinrciqnyggltaenaerlerfkewsdsyeeipcftrcylsemfdfynnl *F tgfnkdgivgvfgrpvyeacrkklelpfesgessckhayegfhcitnkef *F Obp83d (CG15582.m2, OBP83d.m) 83D4 *F ATGCGTATTTACACTACATTACTTAATATATTACAGATGGAAAGCCACCCGTTCACGGTTATTGACAACATGCCAAACAT *F ATCCCCGTCGGCCAAGGATGCAATGAAGGACTGCCTTCAGGATGTCCACCAGGACGAGTGGAAGAGCTTCGATGCCTTCG *F CCTACTATCCTGTCAATGAACCGATTCCGTGCTTCACCCGGTGCTTCGTGGACAAGCTGCATATCTTCGAGGAGAAAACG *F CGTCTTTGGAAACTGGAGGCGATGAAGCAAAACCTGGGCATTCCGGCCAAAGGAGCTCGCATAAGGACCTGCCATCGGCA *F CCGCGGCAGGGACCGATGTGCCACATATTACAAACAGTTCACCTGCTACGCGATGGCTGTT *F meshpftvidnmpnispsakdamkdclqdvhqdewksfdafayypvnepipcftrcfvdklhifeektrlwkleamkqnl *F gipakgarirtchrhrgrdrcatyykqftcyamav *F Obp83e (CG15583.m2, OBP83f.m) 83D4 *F ATGAGTTCCCCTCGTGCGGTTCTAGTCAGCCTGTTCCTGATCTGCAGTCAGGCACTAGCTGACCTTTCTGGTGATGCCCA *F GACTCTGGAAAAGTGCCTGCGGCAACTTAGTTCGCCGGAGAGCATTGCTGGCGATCTTCGAAAGCTGGAACGGTATTCAT *F CTTGGACGCGGGAGGAGGTACCTTGCCTGATGCGCTGCTTGGCCAGAGAAAAGGGCTGGTTCGACGTGGAGGAAAACAAG *F TGGAGGCTCAAGCAACTGACTGAGGACCTGGGCGCCGATGTCTATAACTACTGCAGATTCGAGCTGCGCCGGATGGGGTC *F CGATGGCTGCAGCTTCGCCTATCGGGGACTCAGGTGCCTGAAGCAGGCCGAGATGCATGCGGGCACCAGCCTGAGCACAC *F TGCTACAGTGTTCCCGCCAGCTGAACGCCACCAACGTGGAGCTGCTGCAGTACAGTAAGCTGAAGTCAAAGGAACCTATT *F CCCTGCCTCTTTCAGTGCTTTGCGGATGCCATGGGATTCTACGATCCCGATGGAAACTGGCGGCTGGAAAACTGGAAGCA *F GGCGTTTGGGCCTTCCGGAAATGAGGATCAGTCTTCTGGCGCTGACTACAGTGGCTGTCGACTAAGTGGGACTCAGCGGG *F AGGTGGCGCTAAGCAAGTGCTCGTGGATGTACCATGAGTACAAATGCTGGGAGCGAGTAAATGGGAATAAGCTAGTGGAG *F GACAACGAAGAGCAG *F msspravlvslflicsqaladlsgdaqtlekclrqlsspesiagdlrklerysswtreevpclmrclarekgwfdveenk *F wrlkqltedlgadvynycrfelrrmgsdgcsfayrglrclkqaemhagtslstllqcsrqlnatnvellqysklkskepi *F pclfqcfadamgfydpdgnwrlenwkqafgpsgnedqssgadysgcrlsgtqrevalskcswmyheykcwervngnklve *F dneeq *F Obp83f (CG15583.m1, OBP83g.m) 83D4 *F ATGCAGTCCCAATCCCTCCTGCTGATCGTTGCAGCCGTTGCCACGTTTCTGGTGGCCCAGGTCAGAGCCCAGTGGTTGCC *F CTTGCTGATGGAGACTACGGCCAAGTTCCTGCTGAAGGACCACGCCGACGCAGAGAAGGCGTTCGAGGAGTGCCGTGAGG *F ACTACTACGTGCCGGACGACATCTACGAGAAGTACCTGAACTACGAGTTTCCCGCCCACCGGCGCACCAGCTGCTTCGTC *F AAGTGCTTCCTGGAGAAGCTTGAGCTGTTCTCGGAGAAGAAGGGATTTGACGAGCGCGCCATGATCGCCCAGTTCACCTC *F CAAGAGCAGCAAAGACCTGTCCACGGTCCAGCACGGCCTGGAGAAGTGCATCGACCACAACGAGGCCGAGTCGGATGTCT *F GCACCTGGGCCAACCGAGTCTTCTCCTGCTGGCTGCCCATCAACCGCCACGTGGTGCGTAAGGTCTTCGCC *F mqsqslllivaavatflvaqvraqwlpllmettakfllkdhadaekafeecredyyvpddiyekylnyefpahrrtscfv *F kcfleklelfsekkgfderamiaqftsksskdlstvqhglekcidhneaesdvctwanrvfscwlpinrhvvrkvfa *F Obp84a (PBPRP-4, OBP84a) 84C7 *F ATGTATTCCGCGTTAGTTAGAGCTTGTGCTGTCATTGCTTTTCTGATCTTGAGCCCGAATTGTGCCAGGGCTCTACAGGA *F TCACGCCAAGGATAATGGTGATATTTTCATCATAAACTATGATAGTTTCGATGGCGATGTGGATGACATATCCACCACCA *F CTTCAGCTCCTAGAGAGGCTGACTACGTAGATTTTGACGAGGTTAATCGTAACTGCAATGCTAGTTTCATAACGTCGATG *F ACCAATGTCTTGCAGTTTAATAACACTGGGGATTTGCCAGATGACAAGGATAAGGTAACCAGCATGTGCTATTTTCACTG *F CTTTTTCGAAAAGTCCGGTTTGATGACGGACTATAAGTTAAATACGGATCTGGTGCGCAAATATGTTTGGCCAGCCACTG *F GCGATTCCGTTGAGGCCTGCGAAGCTGAAGGCAAGGACGAGACGAATGCTTGCATGCGGGGCTATGCCATCGTCAAGTGC *F GTGTTTACTAGAGCCCTCACGGATGCTAGAAACAAACCCACTGTA *F mysalvracaviaflilspncaralqdhakdngdifiinydsfdgdvddistttsapreadyvdfdevnrncnasfitsm *F tnvlqfnntgdlpddkdkvtsmcyfhcffeksglmtdyklntdlvrkyvwpatgdsveaceaegkdetnacmrgyaivkc *F vftraltdarnkptv *F Obp85a (CG11732.m) 85A1 *F ATGTCCCCTGGGTCGGTGGTATTTTCCATGTTCCTAACACGTCCCAGTTTAGATAAAGGCAATTCCGAATGTAGGAAAAG *F CCTCAACTTGCCTGCGCACCGGAAATTCAACTTTGCCGAACTATATACGATTAATATGTGCATTGAGGAGTGCAACTTCA *F TAGGCTGTGGCTACATCGAAATCGATCCACCCTTTCGCTTGGATCTGGCCAACATTCGCACCAATCTGCAGACGATTGCA *F CCCCAGCCACAGAATGAATCGATACCATTCCTGGTGGATGCGTATCGGAAATGCGAGCTATTCCGATCGTCGCACGGAAG *F ACGATTCACTCTCCACCTGCCAGATATTGAATTCATCGAGGAACCCTGCAATCCCTTTGCCTTACAAATCACCATATGCG *F TCCGCATCCATGCCATGCAAAAGTGTCCCAGCGAATTTTACGTGGACAGCGACGAGTGCCGATTGGCCAGGGAATACTTC *F ACCCAGTGCGTCGGGGATATTGAAACCAATCTTGCC *F mspgsvvfsmfltrpsldkgnsecrkslnlpahrkfnfaelytinmcieecnfigcgyieidppfrldlanirtnlqtia *F pqpqnesipflvdayrkcelfrsshgrrftlhlpdiefieepcnpfalqiticvrihamqkcpsefyvdsdecrlareyf *F tqcvgdietnla *F Obp93a (CG17284) 93C1 *F ATGAAAACTTCAAATAAAATCGTGTTTTTATTACTACAACTGAATATTTGGCAGTTGAGCTCATGCTGTGATGTTCAAAA *F AAACGACAAGGCCATCAATTCGTGTCGCAAGTCCTTGCTGGGCAATAATTCAACCAATTCGAATGGGGAAGTGCGCAATC *F TTAAGTCGGATAAAGTGGCTTTGCATGCATGTATTGCGGAGTGCTCCTTCAGGACCAATGGATTCTTGTTGAGCAATGGC *F ACTGTTAATACCCAGGCATTGCAGAAGAGCTATCAGCAACGCTACAAAAACGATCCGAATATGTCGCAACTGATGTTGAA *F GAGCCTCAATAGTTGCACGGACTACGCTAGGAAGCGGGTGCAGGAGTTCCAATGGATGCCCAAGAAGGGCGACTGCGACT *F TCTATCCTGCCACCTTGCTGGCCTGCGTCATGGAAAAGGTCTACATCAACTGCCCGACCAGCAAATGGAAAAACACCAGC *F GATTGCACAGCGATGTGGAAATATTTGGTTGCCTGTGATGATGTGGCCAGTAATAAGAAGAAA *F mktsnkivflllqlniwqlssccdvqkndkainscrksllgnnstnsngevrnlksdkvalhaciaecsfrtngfllsng *F tvntqalqksyqqrykndpnmsqlmlkslnsctdyarkrvqefqwmpkkgdcdfypatllacvmekvyincptskwknts *F dctamwkylvacddvasnkkk *F Obp99a (CG18111.m, clot \#14334, OBP99a) 99B9 *F ATGAAGGTTTTCGTTGCCATCTGCGTGCTGATTGGACTGGCCTCCGCCGACTATGTGGTGAAGAACCGACACGACATGCT *F GGCCTACCGCGATGAGTGCGTCAAGGAGCTGGCCGTGCCCGTGGATCTGGTGGAGAAGTACCAGAAGTGGGAGTACCCCA *F ACGACGCCAAGACCCAGTGCTACATCAAGTGCGTCTTCACCAAGTGGGGCCTGTTCGACGTCCAGAGCGGTTTCAACGTG *F GAGAACATCCACCAACAGCTGGTGGGCAACCACGCTGACCACAACGAGGCCTTCCACGCCTCCTTGGCCGCCTGCGTGGA *F CAAGAACGAGCAGGGATCCAATGCCTGCGAGTGGGCCTACCGCGGAGCCACCTGTCTGCTGAAGGAGAACCTGGCCCAGA *F TCCAGAAGAGCCTGGCCCCGAAGGCC *F mkvfvaicvliglasadyvvknrhdmlayrdecvkelavpvdlvekyqkweypndaktqcyikcvftkwglfdvqsgfnv *F enihqqlvgnhadhneafhaslaacvdkneqgsnacewayrgatcllkenlaqiqkslapka *F Obp99b (CG7584, clot \#1575) 99B10 *F ATGCTCAAGTATCTGATCGTAGCTCTGGCCCTCTGTGCTGTGGCCCATGCCGATGACTGGACGCCCAAGACTGGTGAGGA *F GATCAGGAAAATCCGCGTTGATTGCCTCAAGGAGAATCCGCTGAGCAACGACCAGATCTCCCAGCTGAAGAACCTGATCT *F TCCCCAATGAGCCCGACGTGCGCCAGTACCTGACCTGCTCGGCCATCAAGCTGGGCATCTTCTGCGACCAACAGGGCTAC *F CACGCCGACCGCCTGGCCAAGCAGTTCAAGATGGACCTGTCCGAGGAGGAGGCCCTTCAGATCGCCCAGAGCTGCGTGGA *F CGACAACGCGCAGAAGAATCCCACGGATGTGTGGGCCTTTCGCGGACACCAGTGCATGATGGCCAGTAAGATCGGCGACA *F AGGTCAGGGCCTTCGTGAAGGCCAAGGCGGAGGAGGCCAAAAAGAAGGCCGCC *F mlkylivalalcavahaddwtpktgeeirkirvdclkenplsndqisqlknlifpnepdvrqyltcsaiklgifcdqqgy *F hadrlakqfkmdlseeealqiaqscvddnaqknptdvwafrghqcmmaskigdkvrafvkakaeeakkkaa *F Obp99c (CG15505) 99B11 *F ATGAATCACTTGAGACTGGAGATCATCTGCTGGAGCTGCCTGCTTATTGCGATGGCTGTTAGCACGGAAGCTGCTTCTGT *F TTGGAAACTACCCACCGCGCAGATGGTCTACGAGGATCTAGAGAAGTGTCGCCAAGAAAGCCAAGAAGAGGATGCTGCTA *F CCCTGAGGTGTTTGGTTAAGAAACTGGGTCTTTGGACGGATGAGAGTGGCTACAATGCCAGGCGGATAGCAAAGATCTTT *F GCCGGACACAACCAGATGGAGGAGCTGATGCTGGTGGTGGAGCACTGCAACCGGATGGAGCAGGACACGAGCCACCTGGA *F CGACTGGGCCTTCCTGGCCTACAGGTGCGCCACTTCCGGGCAGTTTGGCCATTGGGTCAAGGACTTCATGAGTCAAAAGG *F AAGTGGAACGA *F mnhlrleiicwsclliamavsteaasvwklptaqmvyedlekcrqesqeedaatlrclvkklglwtdesgynarriakif *F aghnqmeelmlvvehcnrmeqdtshlddwaflayrcatsgqfghwvkdfmsqkever *F Obp99d (CG7592, clot \#11054, OBP99b) 99C1 *F ATGAAGGTTCTCATCGTTCTCCTATTGGGTCTGGCCTTTGTCCTGGCCGATCACCATCACCATCACCACGACTATGTGGT *F GAAGACGCACGAGGATCTGACCAACTATCGCACGCAGTGCGTGGAGAAGGTTCACGCCAGCGAGGAGCTCGTGGAGAAGT *F ACAAGAAGTGGCAGTACCCCGATGACGCGGTGACCCACTGCTACCTGGAGTGCATCTTCCAGAAGTTCGGCTTCTACGAC *F ACCGAGCACGGATTCGATGTCCACAAGATCCACATCCAGCTGGCCGGCCCTGGAGTTGAGGTGCACGAATCGGACGAGGT *F GCACCAGAAGATCGCCCACTGCGCCGAGACCCACTCCAAGGAGGGCGACTCCTGCTCGAAGGCCTACCACGCCGGCATGT *F GCTTCATGAACTCCAACCTGCAGCTGGTGCAGCACAGCGTCAAGGTC *F mkvlivlllglafvladhhhhhhdyvvkthedltnyrtqcvekvhaseelvekykkwqypddavthcylecifqkfgfyd *F tehgfdvhkihiqlagpgvevhesdevhqkiahcaethskegdscskayhagmcfmnsnlqlvqhsvkv *F Daria Hekmat-Scafe *F Department of Molecular & Cell Biology *F University of California *F Berkeley, CA 94720 *F TEL (510) 642-8786 *F FAX (510) 643-6791 *F daria@nature.berkeley.edu # *U FBrf0151658 *a Wilder *b B. *t 2002.8.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Aug 20 17:24:52 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: l(2)27Ea125 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Betsy Wilder, University of Pennsylvania Medical Center (8/02). *F l(2)27Ea125 is one of the alleles described in Tiong and Nash, 1990 *F (Genetics 124: 889-897; FBrf0051986) as a 'complementation group G' *F mutation. Since l(2)27Ea125 is lethal, it must correspond to one of the *F l(2)27Ea1 through l(2)27Ea6 alleles. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151659 *a Levis *b R. *t 2002.9.6 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Fri Sep 06 18:33:51 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: New gene CG32386 = ms(3)04202 *F The P-element insertion in the mutant ms(3)04202 is in the newly *F annotated gene CG32386, which has not yet been entered into the *F FlyBase genes database. FlyBase already has a gene named ms(3)04202 *F (synonym ms(3)65E). The gene names should be merged when the record *F for CG32386 is created. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0151660 *a Jackson *b F.R. *t 2002.7.26 *T personal communication to FlyBase *u *F From rob.jackson@tufts.edu Fri Jul 26 20:02:51 2002 *F Subject: Andante *F To: Rachel Drysdale *F Hi Rachel, *F I wanted to add something to the discussion of Andante and Adrocam *F (i.e., your previous emails with Paige Pavlik). It turns out we have *F finally resolved a discrepany concerning dusky (dy) and Andante (And), *F which we had believed for a number of years might be the same gene. We *F now know that they are two different genes within region 10E2-3. We *F recently published some old work on dusky in MGG and are now writing up *F the work on Andante. The 'long and short of it' is that the Andante *F mutant carries two independent EMS-induced mutations, one in dy and one *F in CKII beta. It is the one in CKII beta that is responsible for the *F circadian long-period phenotype. Dusky mutations have no effect on *F rhythmicity. Thus, the name of Andante ought to be CKIIbetaAnd. I *F hope this helps. *F Regards, *F Rob *F \-- *F F. Rob Jackson, Ph.D. *F Department of Neuroscience *F Tufts University School of Medicine *F Boston MA 02111 USA *F Tel \- 617.636.6752 *F Fax \- 617.636.2413 *F From rd120@gen.cam.ac.uk Tue Jul 30 11:52:19 2002 *F To: rd120@gen.cam.ac.uk, rob.jackson@tufts.edu *F Subject: Re: Andante *F Dear Rob, *F All very interesting \- nice that it is becoming clear. So what I need *F to do is extract all reference to Andante from the dusky gene record, *F and move it into that for FBgn0000259. I'd appreciate a bit of help *F with some aspects of this \- so please excuse a few questions.... *F Firstly, I do not believe that any of the molecular information in the *F gene record for dusky applies to Andante, does it .... by 'molecular *F information' I mean these data-bits: *F \*g M84606; AAA28490 *F \*m SPTREMBL:Q24328 *F \*m SPTREMBL:Q9VYU7 *F Is that correct? *F It seems a very close call as to name precedence, but you seem clear *F that CkIIbeta takes priority so I'm happy to go with that. *F Alleles that we currently refer to as dyn1 (FBal0031147), dyn3 *F (FBal0031149), dyn4 (FBal0031150) and dy73 (FBal0003275) all have *F 'locomotor rhythm defective' and all but dy73 have 'eclosion rhythm *F defective' as features of their phenotype. Are these all double *F mutants too? (They do not seem to have a common ancestor, at least not *F according to the info we have on them). They were all discussed in *F FBrf0054173 == Newby et al., 1991, Genetics 128: 571--582 *F and it seems a bit strange to be removing all Andante rhythm stuff from *F the dy gene record while leaving this in. *F Finally \- I will have to name the dy allele on the Andante mutant *F chromosome as something, even though it will have no rhythm phenotype *F associated with it. I would rather it wasn't dyAnd. Do you have a *F line designation, or an amino acid alteration (e.g. Y742N) that I could *F use for the dy allele (e.g. dyY742N or whatever). *F When we have ironed out all these details I'll curate your mail (the *F whole correspondence, probably) as a personal communication from you to *F FlyBase, to bring about the change in a trackable way. *F all the best, *F Rachel. *F From rob.jackson@tufts.edu Fri Aug 16 15:50:28 2002 *F Subject: Re: Andante *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, I will try to answer each of your questions about dy and *F Andante in the appropriate section of your email below. If you still *F have questions, please contact me again. *F Best, *F Rob *F \-- *F F. Rob Jackson, Ph.D. *F Department of Neuroscience *F Tufts University School of Medicine *F Boston MA 02111 USA *F Tel \- 617.636.6752 *F Fax \- 617.636.2413 *F \------------------ *F >Dear Rob, *F > *F >All very interesting \- nice that it is becoming clear. So what I need *F >to do is extract all reference to Andante from the dusky gene record, *F >and move it into that for FBgn0000259. I'd appreciate a bit of help *F >with some aspects of this \- so please excuse a few questions.... *F > *F >Firstly, I do not believe that any of the molecular information in the *F >gene record for dusky applies to Andante, does it .... by 'molecular *F >information' I mean these data-bits: *F >*g M84606; AAA28490 *F >*m SPTREMBL:Q24328 *F >*m SPTREMBL:Q9VYU7 *F >Is that correct? *F All reference to Andante should be removed from the dy record. This *F includes a portion of the mosaic analysis data showing a requirement *F for the rhythm function in the brain. However, you might wish to *F include a note in the CKIIbeta record that the Andante strain carries *F two hits, one in CKIIbeta and one in dy. The most recent and *F appropriate reference for all molecular information about dy is *F DiBartolomeis et al. (2002) Molecular Genetics and Genomics 267: *F 564-576. The entire molecular characterization of dy is in that paper. *F >It seems a very close call as to name precedence, but you seem clear *F >that CkIIbeta takes priority so I'm happy to go with that. *F I think it makes sense to use CKIIbeta since that really is the *F molecular function affected in Andante. *F >Alleles that we currently refer to as dyn1 (FBal0031147), dyn3 *F >(FBal0031149), dyn4 (FBal0031150) and dy73 (FBal0003275) all have *F >'locomotor rhythm defective' and all but dy73 have 'eclosion rhythm *F >defective' as features of their phenotype. Are these all double *F >mutants too? (They do not seem to have a common ancestor, at least not *F >according to the info we have on them). They were all discussed in *F >FBrf0054173 == Newby et al., 1991, Genetics 128: 571--582 *F >and it seems a bit strange to be removing all Andante rhythm stuff from *F >the dy gene record while leaving this in. *F We believe that the dyn1, dyn3, and dyn4 alleles are not really *F independent alleles but rather re-isolates of Andante, based on the *F molecular characterization of polymorphisms in these strains and *F Andante. Although dy73 is reported as being rhythm defective, it is *F not because of a mutation in dy or CKIIbeta. As indicated in Newby et *F al (1991), the factor causing the dy73 rhythm defect does not map to *F the 10E region, but rather to some other part of the X. *F >Finally \- I will have to name the dy allele on the Andante mutant *F >chromosome as something, even though it will have no rhythm phenotype *F >associated with it. I would rather it wasn't dyAnd. Do you have a *F >line designation, or an amino acid alteration (e.g. Y742N) that I could *F >use for the dy allele (e.g. dyY742N or whatever). *F This is a good idea \- the nucleotide change is a G to A transversion *F (characteristic of EMS) that results in a change from a Gly at residue *F 189 to an amber stop codon. You might want to indicate that *F DiBartolomeis et al (2002) refer to this mutant as dyAnd. # *U FBrf0151661 *a Deal-Herr *b M. *c K. *d Cook *t 2002.9.10 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Sep 10 19:42:48 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2R)BSC22 *F Isolation and characterization of Df(2R)BSC22 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC22 was isolated as a P transposase-induced male recombination *F event involving P{EP}betaTub56DEP2640 and P{PZ}mus20902448. The *F deletion was isolated as a cn+-bw+ recombinant chromosome from the *F cross cn1 bw1 females X cn1 *F P{PZ}mus20902448/P{EP}betaTub56DEP2640 bw1 sp1; TMS, Sb1 *F P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints *F 56D7-E3;56F9-12. Df(2R)BSC22 failed to complement 18wDelta7-35 and *F P{lacW}l(2)k00705k00705. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151662 *a Steward *b R. *t 2002.9.13 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Fri Sep 13 19:54:00 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail *F comments: In the synopsis of the shu gene the DNA accession number is *F missing. Thanks for updating it. Ruth Steward *F mailto: flybase-help@morgan.harvard.edu *F realname: Ruth Steward *F reply-to: steward@mbcl.rutgers.edu *F Sent from computer waks-mac-64.rutgers.edu *F From ag24@gen.cam.ac.uk Fri Sep 13 20:01:06 2002 *F To: steward@waksman.rutgers.edu *F Subject: Re: FlyBase Help Mail *F Dear Ruth, *F We were unaware that shu had been identified molecularly. In fact, I'm *F still unaware of it, because I can find nothing in GenBank. Could you *F please give me the accession number? *F Cheers, Aubrey de Grey *F FlyBase *F From steward@waksman.rutgers.edu Fri Sep 13 21:22:48 2002 *F To: Aubrey de Grey *F Subject: Re: FlyBase Help Mail *F When we blast our shu cDNA sequence we only obtain a genomic DNA *F sequence CG 4735. The corresponding cDNA is LD24746. The predicted *F translation product is longer than deduced from our cDNA sequencing. I *F hope this helps and you can update the information for this gene. *F Thanks for the help. *F \-- *F Ruth Steward *F MBB Professor *F Waksman Institute *F 190 Frelinghuysen Rd *F Piscataway, NJ 08854-8020 *F Tel: 732.445.3917 *F FAX: 732.445.5735 *F From ag24@gen.cam.ac.uk Fri Sep 13 21:29:24 2002 *F To: steward@waksman.rutgers.edu *F Subject: Re: FlyBase Help Mail *F Hi, *F That's a great start \-- but since your cDNA sequence is not in GenBank *F we have no published (or even electronically published) data to link to. *F However, if you wish, we can use your latest email as a source for the *F merging of CG4735 (which we have as identified with LD24746) into shu. *F Just let us know if that's what you'd like; if so, it will be apparent *F on FlyBase (as a 'recent updates' entry for shu) within a few days. *F Cheers, Aubrey *F From steward@waksman.rutgers.edu Fri Sep 13 21:43:54 2002 *F To: Aubrey de Grey *F Subject: Re: FlyBase Help Mail *F If that is OK with you it is OK with me. Thanks *F \-- *F Ruth Steward *F MBB Professor *F Waksman Institute *F 190 Frelinghuysen Rd *F Piscataway, NJ 08854-8020 *F Tel: 732.445.3917 *F FAX: 732.445.5735 # *U FBrf0151663 *a Roote *b J. *t 2002.9.18 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Wed Sep 18 11:15:09 2002 *F To: Rachel Drysdale *F Subject: FBrf0145636 update *F Dear Rachel, *F An update of my pers comm FBrf0145636: *F Df(2R)ED1 is also lethal with RhoGEF204291 *F Df(3R)ED2 is lethal with l(3)S007302 and Df(3R)Cha9 *F Df(2L)ED3 has been confirmed by PCR. *F from John # *U FBrf0151664 *a Simonova *b O. *t 2002.8.21 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Wed Aug 21 16:25:43 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase Help Mail *F comments: 1th comment. The full name of lawc locus is leg arista wing *F complex. *F 2nd comment. l(1)520 \- is the lethal embryonic allele of lawc gene. *F mailto: flybase-help@morgan.harvard.edu *F realname: Simonova Olga *F reply-to: osimonova.hotmail.com *F Sent from computer 195.178.221.125 # *U FBrf0151665 *a Montell *b D. *t 1998.11.25 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Fri Sep 20 01:39:59 2002 *F Subject: Insertions of ras and ral constructs *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Denise Montell, Johns Hopkins School of Medicine *F To: Bloomington Drosophila Stock Center *F Subject: Insertions of ras and ral constructs *F Dated: 25 November 1998 *F Information communicated: *F 1. P{UAS-ras.N17}TL1 *F Chromosome 1 insertion, viable and fertile. *F This dominant negative allele of mammalian ras works better than the *F Drosophila allele carried in P{UAS-Ras85D.N17}. *F 2. P{UAS-Ras85D.N17}TL1 *F Chromosome 1 insertion, viable and fertile. *F 3. P{UAS-Ras85D.V12}TL1 *F Chromosome 3 insertion, viable and fertile. *F 4. P{UAS-ralA.72L}TL1 *F Chromosome 1 insertion, viable and fertile. *F This construct carries mammalian ralA. *F 5. P{hs-ralA.N28}TL1 *F Chromosome 3 insertion, lethal. *F This construct carries mammalian ralA. # *U FBrf0151666 *a Luo *b L. *t 2002.9.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Sep 20 17:38:52 2002 *F Envelope-to: rd120@gen.cam.ac.uk *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Luo insertions *F Mime-Version: 1.0 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Liqun Luo, Stanford University (9/02). *F The following are homozygous/hemizygous viable and fertile X chromosome *F insertions: *F P{UAS-rok.CAT}2.1 *F P{UAS-Rac1.Myc}2.4 *F P{UAS-Myc-Rac1.F37A}7.2 *F The following are homozygous viable and fertile second chromosome insertions: *F P{UAS-rok.CAT}7.1 *F P{UAS-rok.CAT-KG}2B1 *F P{UAS-Myc-Rac1.Y40C}2 *F P{UAS-myc-RhoGAPp190.B}2.1 *F P{UAS-RhoGAP93B-dsRNA}3.1 *F The following are homozygous viable and fertile third chromosome insertions: *F P{alphaTub84B-rok.W}3C *F P{UAS-rok.CAT}3.1 *F P{UAS-rok.CAT-KG}3 *F P{UAS-myc-RhoGAPp190.R1389L}2.2 *F P{Rac1+t3.6}1.7.2 is an insertion on 2R. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151667 *a Kadonaga *b J. *t 2002.9.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Sep 20 19:49:10 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{DPE-GFP.TATA-GFP}e90 insertion and derivatives *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jim Kadonaga, University of California at San Diego (9/02). *F P{DPE-GFP.TATA-GFP}e90 is a homozygous viable and fertile, third *F chromosome insertion which traps a Downstream Promoter Element *F (DPE)-specific enhancer. P{TATA-GFP}e90 and P{DPE-GFP}e90 are *F cre-induced and FLP-induced derivatives of *F P{DPE-GFP.TATA-GFP}e90, respectively. The recovery of *F P{DPE-GFP.TATA-GFP} insertions and the generation of recombinant *F derivatives was described in Butler and Kadonaga, 2001 (Genes Dev. *F 15:2515--2519; FBrf0139667). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151668 *a Luo *b L. *t 2002.9.23 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Sep 23 17:59:18 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{alphaTub84B-rok.W}2A insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Liqun Luo, Stanford University (9/02). *F P{alphaTub84B-rok.W}2A is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151669 *a Herr *b D. *t 2002.10.4 *T personal communication to FlyBase *u *F From dherr@sunstroke.sdsu.edu Fri Oct 04 19:03:46 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: gene nomenclature *F Hello again Rachel, *F We have demonstrated that two Drosophila cDNAs (LD11247 and RE64552) are *F both able to fully complement a yeast sphingosine kinase mutant indicating *F that CG32484 (CG2159) and CG1747 both have sphingosine kinase *F activity. This is also suggested by their high degree of sequence *F similarity to human, mouse, and yeast sphingosine kinases. Therefore, we *F suggest the following changes to the gene nomenclature: *F CG1747 changed to Sphk1 *F CG32484 changed to Sphk2 *F This work will be discussed in a forthcoming publication tentatively titled: *F Identification and analysis of Drosophila sphingosine kinases. *F Deron Herr, Michael Creason, Henrick Fyrst, Karie Heinecke, Julie Saba, and *F Greg Harris. *F Thank You, *F Deron Herr *F San Diego State University *F (619)594-5038 # *U FBrf0151670 *a Hirota *b Y. *t 2002.10.1 *T personal communication to FlyBase *u *F From yhirota@sc.itc.keio.ac.jp Tue Oct 01 06:23:36 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: about our gene *F To whom concerned, *F We performed cloning of tincar gene and our paper was accepted for *F Mechanisms of Development yesterday. I found mistakes in spelling of tincar *F described in Flybase. Because of tincar is not a dominant gene, it should *F be 'tincar', not 'Tincar'. Additionally, we call tincar 'tinc' for short. *F Would you correct and add these information in Flybase? *F Thank you for your consideration, *F Sincerely yours, *F Yuki Hirota *F HIROTA,Yuki *F Department of Physiology *F Keio University School of Medicine *F 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. *F Phone 81-3-5363-3746 *F Fax 81-3-3357-5445 *F e-mail: yhirota@sc.itc.keio.ac.jp # *U FBrf0151671 *a Bejsovec *b A. *t 2002.10.7 *T personal communication to FlyBase *u *F From bejsovec@duke.edu Mon Oct 07 19:44:55 2002 *F To: gelbart@morgan.harvard.edu *F Subject: dAPC2 curation *F Cc: flybase-help@morgan.harvard.edu *F Dear Bill, *F I've just donated another allele of dAPC2, d40, to the *F Bloomington Stock Center and noticed that the Flybase curation for *F both this allele and the first dAPC2 allele, delta-S, was a little *F inaccurate. The original allele my lab isolated, delta-S, is *F temperature sensitive but even at the restrictive temperature it is *F homozygous viable in the first generation. Homozygous mutant flies *F mated to each other will produce 100% lethal embryos, all showing the *F excess naked cuticle typical of ectopic Wg pathway activity. At 18 *F degrees, the homozygous mutant (maternal/zygotic) embryos are *F essentially wild-type and the flies can be maintained as a homozygous *F stock. This is experimentally convenient, but we have since found *F that the phenotype at restrictive temperature is not equivalent to a *F null phenotype. We've more recently isolated a more severe allele, *F d40. This allele is not temperature sensitive, but is still *F homozygous viable in the first generation. Homozygous mutant flies *F mated together produce embryos showing a more complete excess naked *F cuticle phenotype than that produced by the delta-S stock. Thus we *F consider this a more severe allele for the locus, although we suspect *F it is still not a null since we can detect protein with the dAPC2 *F antibody. The molecular lesion in d40 is a stop codon replacing *F cysteine 677, so it is a fairly late truncation. *F I've sent this allele to Eric Wieschaus' lab and shared it *F with Mark Peifer in a collaboration. The first published reference *F to the d40 allele is: *F McCartney, B.M., McEwen, D.G., Grevengoed, E., Maddox, P., Bejsovec, *F A., Peifer, M. (2001) Drosophila APC2 and Armadillo participate in *F tethering mitotic spindles to cortical actin. Nature Cell Biol. *F 3(10):933--938 *F The reference to which Flybase attributes d40 (Ahmed et al. *F 2002) is a later publication from Eric's lab where they use it to *F show that APC1 and APC2 are partially redundant, which accounts for *F the F1 viability of the APC2 mutations. Mark's lab has a similar *F paper showing this, also using the d40 allele, which has just come *F out: *F Akong K, Grevengoed E, Price M, McCartney B, Hayden M, DeNofrio J, *F Peifer M. (2002) Drosophila APC2 and APC1 play overlapping roles in *F Wingless signaling in the embryo and imaginal discs. Dev Biol. *F 250(1):91 \- 100. *F Could the d40 allele entry be updated to include the *F molecular data (personal communication from me), the correct primary *F reference, an indication that this is the most severe allele *F available at the moment, and eventually the Bloomington stock number *F when Kevin and Kathy have it ready? *F Thanks! *F \-- Amy *F \-- *F Amy Bejsovec, Ph.D. *F Duke University *F Dept. of Biology/DCMB Group *F Box 91000 *F B336 LSRC, Research Dr. *F Durham, NC 27708-1000 *F (919) 613-8162 office email: bejsovec@duke.edu *F (919) 613-8163 lab FAX: (919) 613-8177 *F (919) 613-8153 fly room # *U FBrf0151672 *a Merdes *b G. *t 2002.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Oct 07 18:04:07 2002 *F To: Rachel Drysdale (Genetics) *F Subject: APP constructs and insertions *F The following information was provided by Gunter Merdes, University of *F Heidelberg (9/02). *F P{UAS-Hsap\APP.695SPA4CT.T:Myc}XX and P{UAS-Hsap\APP.695DeltaCT.T:Myc}61 *F are homozygous viable and fertile second chromosome insertions. Their *F construction and transformation were described in Fossgreen et al. (PNAS *F 95: 13703-13708, 1998; FBrf0105227). *F After Fossgreen et al. was published, constructs similar to *F P{UAS-Hsap\APP.695} and P{UAS-Hsap\APP.695-Swedish} were made with *F N-terminal Myc tags. The constructs are P{UAS-APP695-N-myc} and *F P{UAS-APP695-Swedish-N-myc}. *F P{UAS-APP695-N-myc}TW6 is a homozygous viable and fertile second *F chromosome insertion. *F P{UAS-APP695-Swedish-N-myc}TS23 is a homozygous viable and fertile *F third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151673 *a Levis *b R. *t 2002.10.2 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Wed Oct 02 18:30:31 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: Zfrp8 = CG3260? *F Zfrp8 and CG3260 are currently treated as separate genes in FlyBase. *F I haven't looked at the literature on Zfrp8, but it seems likely that *F they are synonyms since the Zfrp8k13705 allele has a P-element *F insertion in the 5' exon of CG3260. Perhaps one of the curators at *F FlyBase could investigate this more carefully. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0151674 *a Levis *b R. *t 2002.10.8 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Tue Oct 08 21:10:27 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: l(3)05712 is an allele of sar1 *F The P-element in the l(3)05712 mutant is inserted in the 5' exon of *F the sar1 gene. There is a stock of this mutant in the Bloomington *F stock center (11669). The FlyBase report for the sar1 gene does not *F record this mutant as an allele. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0151675 *a Prochnik *b S. *t 2002.10.14 *T personal communication to FlyBase *u *F From simonp@fruitfly.org Mon Oct 14 19:31:48 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: renaming a gene *F Hi Cambridge, Rachel *F Thanks for your email explaining the renaming protocol *F I have named CG32208 825-Oak *F it's a long story, and like i said to Suzi when she asked why, a brief *F if cryptic explanation is that you'll find out one Thursday night. *F I enjoyed seeing you in cambridge during my brief stay at flybase/mine. *F see you soon *F All the Best *F Simon *F \-- *F Simon Prochnik, PhD. *F Postdoctoral Curator, *F Berkeley Drosophila Genome Project, *F Life Sciences Division, *F Lawrence Berkeley National Lab, *F One Cyclotron Road, Mailstop 64-121, *F Berkeley, CA 94720 *F tel (510) 486 6836 *F fax (510) 486 6798 *F cell (415) 310 6502 *F home (415) 552 9594 # *U FBrf0151676 *a Smith *b C. *t 2002.10.14 *T personal communication to FlyBase *u *F From cdsmith@fruitfly.org Mon Oct 14 22:13:23 2002 *F To: 'Rachel Drysdale (Genetics)' *F Subject: RE: naming a gene \- ACTION REQUIRED THIS WEEK *F Rachel, *F Here is my official request to for my named gene- *F I would like to rename the dicistornic cassette CG33075 to Tyler+Shawn (note *F that the annotation id cannot actually be 'Tyler+Shawn' until the new *F schema. CG14208 should be called Tyler. CG14209 should be called Shawn. *F These both have GO headings as mitochondrial transporters which I thought *F was appropriate since I am naming these genes after my parents newly adopted *F and energetic 9 and 11 year old boys. *F Thanks, *F Chris Smith # *U FBrf0151677 *a Lewis *b S. *t 2002.10.22 *T personal communication to FlyBase *u *F From suzi@fruitfly.org Tue Oct 22 07:44:16 2002 *F From: Suzanna Lewis *F Subject: Re: naming a gene \- ACTION REQUIRED THIS WEEK *F To: Rachel Drysdale (Genetics) *F the name for my gene is PrayForElves *F it is this gene: *F *F It is the middle of the night (2:38 to be precise), *F I am away from friends and family, It has been *F this way for over 2 years, I can't sleep because *F of all the work there is yet to do, and there *F is no end in sight. So when do the magic *F little elves appear out of nowhere and get *F everything done? *F \-S *F p.s. I am serious. # *U FBrf0151680 *a Salvaterra *b P. *t 2002.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Oct 30 02:33:00 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Nrv2-GAL4.S} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Paul Salvaterra, Beckman Research Institute of the City of *F Hope (9/02). *F P{Nrv2-GAL4.S}3 is a homozygous viable and fertile second chromosome insertion. *F P{Nrv2-GAL4.S}8 is a homozygous viable and fertile third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151681 *a Salvaterra *b P. *t 2002.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Oct 30 02:36:51 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Cha-GAL4.W}19B *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Paul Salvaterra, Beckman Research Institute of the City of *F Hope (9/02). *F P{Cha-GAL4.W}19B is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151682 *a Deal-Herr *b M. *c K. *d Cook *t 2002.11.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Nov 08 20:13:45 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3R)BSC24 *F Isolation and characterization of Df(3R)BSC24 *F Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC24 was isolated as a P transposase-induced male recombination *F event involving P{EP}EP707EP707 and P{EP}EP3243. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females X CyO, H{PDelta2-3}HoP2.1/+; st1 *F EP(3)3243/P{EP}EP707EP707 ca1 males. Polytene chromosome squashes *F showed the breakpoints 85C4-9;85D12-14. Df(3R)BSC24 failed to complement *F pum13, neur1, and osk6. The deficiency chromosome retains the *F miniwhite marker from one or both P{EP} elements. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151683 *a Luo *b L. *t 2002.10 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Nov 08 20:58:26 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-myc-RhoGAPp190.mut}7.1 *F The following information was provided to the Bloomington Stock Center by *F Liqun Luo, Stanford University (10/02). *F P{UAS-myc-RhoGAPp190.mut}7.1 is a homozygous viable and fertile, second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151684 *a Schubiger *b G. *t 2002.10 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 11 20:28:35 2002 *F To: Rachel Drysdale (Genetics) *F Subject: P{AGiR-Gal4}3 *F The following information was provided by Gerold Schubiger, University of *F Washington (10/02). *F P{AGiR-Gal4}3 is a third chromosome insertion. Its isolation was described *F in Gibson et al., 2002 (Dev. Cell 3: 451-460). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151685 *a Bourouis *b M. *t 2002.10 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 11 20:45:14 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GAL4-vg.M}2 and P{UAS-lacZ.Hsp70}2 *F The following information was provided to the Bloomington Stock Center by *F Marc Bourouis, Universite de Nice (10/02). *F P{GAL4-vg.M}2 is a second chromosome insertion originating in Simmonds et *F al., 1995 (Nature 376:424-427; FBrf0082604). *F P{UAS-lacZ.Hsp70}2 is a second chromosome insertion originating in Hinz et *F al., 1994 (Cell 76:77-87; FBrf0068528). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151686 *a Raabe *b T. *t 2002.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Nov 12 15:49:06 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{lacW}dosP115 *F The following information was provided to the Bloomington Stock Center by *F Thomas Raabe, University of Würzburg (11/02). *F The P element construct present in the dosP115 allele is P{lacW}. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151687 *a Deal *b J. *c R. *d Andrade *e K. *f Cook *t 2002.11.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Nov 12 15:56:29 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC23 *F Isolation and characterization of Df(3L)BSC23 *F Jennifer Deal, Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC23 was isolated as a P transposase-induced male recombination *F event involving P{lacW}dosP115 and P{lacW}Hsp83j5C2. The deletion was *F isolated as a rhove-1-e1 recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{lacW}dosP115/P{lacW}Hsp83j5C2 e1 males. Polytene chromosome *F squashes showed the breakpoints 62E8;63B5-6. Df(3L)BSC23 failed to *F complement aly1 and l(3)63Bg1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151688 *a Golic *b K. *t 2002.10.25 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Nov 11 22:00:35 2002 *F To: Rachel Drysdale (Genetics) *F Subject: p53 alleles *F Hi Rachel-- *F I've attached a copy of a letter from Kent Golic describing two alleles of *F p53, p5311-1B-1 and p535A-1-4, generated in his paper Rong et al. Genes *F Dev. 2002 16(12):1568--1581 (FBrf0149003). *F Could you make allele entries for these? *F Many thanks! *F Kevin *F \--------------------------------------------------------------------- *F October 25, 2002 *F Hi Kathy, *F We've had many, many requests for the p53 mutant stocks so we decided to *F send them to the stock center. I hope you'll be able to take care of them. *F If there *F are any problems, please let me know. There's no obvious phenotype for the *F p53- alleles. They can be verified by PCR. *F p535A-1-4 is a deletion. These primers: *F P53-C1: 5'-AGCTAATGTGACTTCGCATTGAACAAA-3' *F P53-C2: 5'-TCGATAAACATTGGCTACGGCGATTGT-3' *F will give a 4kb product from the deletion and a 7.3kb product from the wild-type *F allele. *F The other allele, p5311-1B-1 results from introduction of a stop mutation *F into the *F coding sequence. It can be verified by allele-specific PCR with the following *F three *F primers: *F (1) 5'-GTTCGCCTGGATCTTAATTA-3'; *F (2) 5'-GTTCGCCTGGATCTGAATGT-3'; and *F (3) 5'-AATCGCTGCATGCGGTAGTA-3'. *F Primers 1 and 3 generate a 1.3kb fragment specifically from the mutant allele. *F Primers 2 and 3 generate a 1.3kb fragment specifically from the wild-type *F allele. *F Thanks, *F Kent *F Kent Golic, *F Department of Biology *F 257 South 1400 East Rm 201 *F Salt Lake City, Utah 84112-0840 *F PHONE (801) 581-6517 FAX (801)581-4668 *F www.biology.utah.edu *F \--------------------------------------------------------------------- *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0151689 *a Wilkin *b M.B. *c M.N. *d Becker *e D. *f Mulvey *g I. *h Phan *i A. *j Chao *k K. *l Cooper *m H.J. *n Chung *o I.D. *p Campbell *q M. *r Baron *q R. *r MacIntyre *t 2002.11.25 *T personal communication to FlyBase *u FlyBase error report for dp. *F From mbaron@man.ac.uk Mon Nov 25 16:26:45 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: dp gene structure *F Hi Rachel, *F We had a look at this and it looks fine . Thanks for sorting this out. *F Best wishes, *F Martin *F >M.B. Wilkin, M.N. Becker, D. Mulvey, I. Phan, A. Chao, K. Cooper, *F H-J. Chung, I.D. Campbell, M. Baron and R. MacIntyre *F A version of this information has been previously published in FBrf0131182. *F The predicted cDNA sequence of dumpy: *F Sites of introns removed from the genomic sequence AE003576 are *F indicated with a vertical line |. All exons were shown experimentally *F to be able to splice directly on to adjacent exons. The sequence is *F split between the 5'and 3'coding regions. In between lies an *F additional 11 kb of dumpy coding region consisting entirely of highly *F conserved 306 bp tandem repeats, encoding the `Pigs-Feast' repeat. *F Because there is virtual identity between these repeats it has not been *F possible to contig this region. The predicted initiating methionine *F codon and the predicted stop codon are marked by __. Altogether, the dumpy *F gene encodes a 70kb cDNA which codes for a 2.5 MDa extracellular matrix *F protein. *F 5'half of dumpy: *F TGGACAGTGTGTTAAGTGTTAAGTTTGGCTCCAGCAGTCCGGATAGATCATCCGAGCGGAGATCGTTGCCTTATTTGAA *F TAGGCGGGGAATCTAACATCTATC *F GCCTAATTGCAGTGAAAGTTGCCGGGCATTTTTCAGCTATTTTACCCTGTGAAAATTCCGAAAAACCAAACGCGCGCAA *F AGCAATCCAAAGTGTTCATAAGAA *F AAGCGTTTAATATTGTTAAGTACAACAATACGTTTTAATACAAATATTCAAGTGTCTGTGAGTCTGCCAAAGTCGTCGC *F GCAGTGTGTGTGTGTGTGTCTTTC *F TGCGCTTTTGACGGCAAATCGCTTTTGGCAGCTGCTTTGGCAGCAGCCAAGTAAGAAACAAGGCTTTTTTGCTGGCGTC *F GATCTTCGTTGGCAGCTGTTGGCA *F GTATGAACTGATTTTGGCCGAAAGTTGATGAATAAAATG|CAAAA_ATG_AAAATATTTTTGCCACTAGTCACGTGGAT *F AGTGCTACTACTCTCGAGTGCA|GTAC *F ATTCACAATATTCACAACAACCGCAACCATTCAAGACGAACTTAAGGGCGAATAGTCGGTTTCGCGGCGAGGTCTTCTA *F CCTGAACCTCGAGAATGGCTACTT *F TGGCTGCCAAGTCAACGAGTCCACCGAATATTTGCAGCTATTCAACCTATCGAAACTATGCGACGGTACCCAAGATTGC *F TTCCTGGGTGCCGATGAGTTGAGC *F AAAGAGCTCAAATGCACAA|ATGACTGTGATAAGGATGGCACCAAGTGCACACATGGCGCGTGTTTGAACGGCGTCTGC *F CATTGCAATGACGGCTACGGCGGCT *F GCAATTGCGTGGACAAAG|ATGAGAACGAATGCAAACAGCGGCCGTGCGACGTGTTCGCCCATTGCACAAACACACTGG *F GCAGCTTCACGTGTACCTGCTTTCC *F GGGCTATCGAGGCAATGGTTTTCATTGTGAAG|ACATTGACGAATGCCAAGATCCCGCGATAGCAGCGCGATGCGTGGA *F GAACGCCGAGTGCTGCAACCTGCCG *F GCGCACTTCCTCTGCAAGTGCAAGGATGGATACGAGGGCGACGGCGAAGTTCTGTGCACGGATGTGGACGAGTGTCGCA *F ATCCGGAGAACTGCGGTCCCAACG *F CTCTGTGCACCAACACGCCGGGCAATTACACATGCTCCTGCCCGGACGGCTATGTGGGCAACAATCCGTATCGGGAGGG *F ATGCCAGGATGTGGACGAGTGCTC *F GTACCCGAATGTCTGCGGACCCGGTGCCATATGTACGAATCTCGAGGGCAGTTATCGCTGCGACTGCCCGCCGGGATAC *F GACGGCGATGGACGCTCGGAGTCC *F GGTTGCGTGGATCAGGATGAGTGCGCACGGACGCCCTGCGGCCGGAACGCGGATTGTTTGAACACGGACGGCAGCTTCC *F GCTGCTTGTGTCCGGACGGCTACA *F GTGGTGATCCCATGAACGGCTGCGAGG|ATGTCGATGAATGCGCCACTAACAATCCATGCGGTTTGGGTGCTGAATGCG *F TGAACCTGGGCGGTAGCTTCCAGTG *F CCGCTGTCCCTCCGGCTTTGTGTTGGAGCACGATCCGCACGCGGATCAGTTGCCCCAGCCGCTGAACACCCAACAATTG *F GGCTACGGGCCGGGGGCGACGGAC *F ATTGCGCCCTATCAAAGGACCTCCGGCGCCGGTCTGGCATGCCTGGACATCGATGAGTGCAATCAGCCGGATGGAGTGG *F CCAAGTGCGGCACCAATGCCAAGT *F GCATTAACTTTCCCGGCTCATACCGCTGCCTGTGTCCGAGTGGATTCCAAGGACAGGGCTATTTGCACTGCGAGA|ACA *F TCAACGAGTGTCAGGATAATCCGTG *F TGGGGAGAATGCGATCTGTACGGACACCGTTGGCAGCTTCGTGTGCACATGCAAACCGGACTATACCGGTGATCCGTTC *F CGCGGATGTGTGGATATCGATGAG *F TGTACCGCGTTGGATAAGCCCTGTGGACAGCATGCGGTATGCGAAAATACCGTTCCTGGCTACAATTGCAAGTGCCCGC *F AGGGATATGATGGCAAACCCGATC *F CCAAGGTGGCCTGCGAACAGGTGGATGTCAATATACTGTGCAGCAGTAACTTCGATTGTACCAACAATGCGGAGTGCAT *F TGAGAATCAGTGCTTCTGCTTGGA *F TGGATTCGAACCGATTGGATCCAGTTGTGTGGACATCGATGAGTGTCGCACCCATGCCGAGGTGTGTGGTCCCCATGCC *F CAGTGCCTGAATACGCCCGGATCG *F TACGGCTGTGAATGCGAGGCGGGATATGTGGGTAGTCCGCCAAGAATGGCGTGCAAGCAGCCATGCGAGGATGTTCGCT *F GTGGAGCCCACGCCTACTGCAAAC *F CGGACCAGAATGAAGCCTACTGTGTGTGCGAGGATGGATGGACCTACAACCCGAGCGATGTCGCTGCGGGCTGTGTGGA *F CATCGATGAGTGCGATGTGATGCA *F TGGACCTTTCGGAAGCTGTGGCCAAAATGCCACGTGTACAAACAGTGCCGGAGGATTTACATGTGCCTGCCCACCTGGA *F TTCTCGGGAGATCCGCACTCTAAA *F TGCGTCGATGTGGATGAGTGCCGAACCGGAGCCAGCAAGTGTGGAGCTGGAGCTGAATGTGTGAACGTACCCGGTGGTG *F GATACACCTGTCGTTGCCCCGGGA *F ACACCATTGCCGATCCGGATCCTAGTGTGAGGTGTGTGCCCATTGTCAGCTGCTCCGCTAACGAAGACTGCCCGGGTAA *F CTCCATATGTGATGCTACAAAGCG *F CTGCCTGTGTCCGGAACCGAATATTGGTAATGACTGCCGTCATCCGTGCGAAGCCCTCAACTGTGGAGCACATGCCCAG *F TGCATGCTGGCCAATGGACAGGCT *F CAGTGCCTGTGTGCTCCCGGATACACAGGAAACTCTGCCCTTGCAGGAGGCTGTAATGACATCGATGAATGTCGCGCCA *F ATCCCTGCGCCGAGAAGGCAATCT *F GTTCGAATACAGCCGGAGGGTATCTATGTCAGTGTCCAGGAGGATCCAGTGGAGATCCATACCGCGAGGGGTGTATTAC *F CTCAAAGACGGTGGGCTGCTCTGA *F TGCCAATCCTTGCGCGACTGGCGAGACTTGCGTCCAAGACTCATACACGGGCAATAGCGTATGCATTTGTCGACAGGGC *F TATGAGCGAAATTCCGAGAACGGA *F CAGTGCCAGGATGTGGATGAATGCTCGGTGCAAAGAGGAAAGCCCGCTTGTGGACTAAATGCTCTCTGTAAGAATTTGC *F CTGGCAGCTACGAGTGCCGCTGCC *F CACAGGGTCATAACGGAAATCCCTTCATCATGTGCGAGATCTGCAATACGCCTGAGTGCCAGTGTCAGTCACCGTACAA *F GCTTGTTGGAAATAGTTGTGTTCT *F CTCCGGTTGCTCCAGTGGACAGGCCTGTCCCAGCGGAGCTGAGTGCATCTCCATTGCTGGAGGTGTTAGCTACTGTGCC *F TGTCCCAAGGGTTACCAAACCCAA *F CCGGATGGAAGCTGTGTGGATGTCGACGAGTGCGAAGAACGGGGCGCGCAATTGTGTGCTTTTGGCGCTCAGTGCGTGA *F ACAAGCCTGGAAGCTATAGTTGCC *F ACTGTCCCGAGGGTTACCAGGGAGATGCCTACAACGGATTATGTGCACTCGCACAAAGGAAATGCGCTGCCGATAGGGA *F GTGCGCTGCCAACGAAAAATGCAT *F CCAGCCAGGAGAGTGTGTTTGCCCGCCTCCTTATTTCTTGGACCCGCAGGACAACAACAAGTGCAAGAGCCCTTGCGAA *F CGCTTCCCATGTGGCATTAACGCT *F AAGTGTACGCCCTCGGATCCTCCACAGTGCATGTGCGAGGCTGGATTCAAGGGAGATCCACTGCTGGGATGTACAGATG *F AAGACGAGTGCTCCCATCTCCCAT *F GTGCCTATGGAGCCTACTGTGTAAACAAAAAGGGCGGATACCAGTGCGTGTGCCCCAAGGATTATACCGGAGATCCGTA *F CAAGAGTGGATGCATCTTCGAGAG *F CGGCACGCCGAAGAGCAAGTGTCTGAGCAACGATGATTGCGCAAGTAATCTAGCCTGCTTGGAAGGTAGCTGCGTAAGT *F CCATGCAGCAGTCTTCTGTGTGGA *F TCGAATGCCTACTGTGAGACAGAGCAACATGCTGGCTGGTGTCGCTGTCGTGTGGGATACGTTAAAAATGGCGATGGGG *F ACTGCGTGTCTCAGTGCCAGGACG *F TGATCTGCGGCGATGGTGCTCTATGCATTCCAACCAGTGAGGGACCCACCTGTAAGTGCCCGCAAGGACAGCTCGGCAA *F TCCCTTCCCCGGCGGCAGCTGCAG *F CACGGATCAGTGTTCAGCCGCCAGGCCTTGTGGCGAGCGGCAGATTTGCATCAATGGAAGATGCAAGGAGCGCTGCGAG *F GGTGTGGTCTGTGGCATCGGAGCC *F ACCTGCGATAGAAACAATGGAAAGTGCATCTGCGAGCCGAACTTTGTGGGTAATCCCGACTTGATCTGTATGCCACCCA *F TCGAGCAGGCCAAGTGCTCTCCAG *F GATGTGGAGAGAACGCTCACTGCGAGTACGGATTGGGTCAGAGCCGATGCGCCTGTAACCCGGGAACCTTCGGTAATCC *F CTATGAAGGCTGTGGAGCACAAAG *F CAAGAACGTCTGTCAGCCGAATAGCTGTGGACCCAATGCCGAATGCCGTGCTGTGGGCAACCACATATCCTGTCTTTGC *F CCACAGGGCTTCAGTGGTAATCCC *F TACATTGGCTGCCAGGATGTGGATGAGTGTGCCAACAAACCATGTGGTCTGAATGCTGCCTGTTTGAATAGAGCCGGAG *F GATTCGAGTGCTTGTGTCTTTCGG *F GACACGCCGGAAATCCCTACAGTAGTTGTCAACCCATCGAGAGCAAGTTCTGCCAAGATGCGAACAAGTGCCAGTGCAA *F CGAACGAGTGGAGTGTCCGGAAGG *F CTACAGTTGCCAAAAGGGACAGTGCAAGAACCTCTGTTCCCAGGCTTCGTGTGGCCCAAGGGCCATCTGCGATGCCGGT *F AATTGCATCTGCCCCATGGGCTAC *F ATTGGAGATCCCCACGACCAAGTGCACGGTTGCAGCATTCGCGGTCAGTGTGGCAATGATGCGGACTGCCTGCACTCTG *F AGATCTGTTTCCAGTTAGGCAAGG *F GTCTGCGCAAATGTGTGGACGCATGCTCCAAGATTCAGTGTGGACCCAATGCCCTCTGCGTGTCCGAGGATCATCGATC *F TTCCTGTATATGCTCTGACGGATT *F CTTCGGAAATCCAAGCAACCTACAGGTGGGATGCCAGCCGGAAAGAACAGTTCCCGAGGAAGAGGATAAATGTAAATCG *F GATCAGGACTGTAGTCGAGGATAC *F GGTTGTCAGGCCAGCGTTAACGGCATCAAGGAGTGCATTAATCTCTGCTCCAATGTTGTCTGCGGACCCAACGAGCTGT *F GCAAGATCAACCCAGCTGGGCATG *F CGATTTGTAATTGCGCCGAGAGCTATGTCTGGAACCCAGTGGTCTCTTCCTGCGAAAAGCCTTCACTGCCAGACTGCAC *F CAGTGACGCTAATTGCCCGGATGC *F TTCTGCCTGTCGTCCTGATGTCTTGGGAGTCCTTAAGTGCGTCGCCATCTGTGATGCCTTCACGTGCCCGGCCAACTCG *F GTTTGCGTAGCCCGTCAGCACCAG *F GGACGATGTGATTGTCTCAACGGGTTTGTGGGTAACCCCAACGATAGGAACGGCTGTCAGCCGGCACAGAAACATCACT *F GCAGAAACCATGCAGAGTGCCAGG *F AGTCGGAAGCCTGTATTAAGGATGAATCTACCCAGACTCTCGGATGTCGTCCAGCTTGTGATACGGTGAAATGTGGACC *F CCGAGCCGTTTGCGTCACCAACAA *F TCATCAGGCGCAGTGTCAGTGTCCACCTGGACCTTTCGCCGGAGATCCATACGATCCATTTAATGGCTGCCAGAGTGTT *F CCTTGTGTTTACAACCATGACTGC *F CCACCCAGCCAGATGTGCAACCGTATGACACACACATGCTTCGATGTGTGCGACGAGGAGTCCTGCGGTGACAATGCCA *F TCTGTTTGGCTGAGGATCATCGAG *F CCGTCTGCCAGTGTCCACCTGGATTTAAGGGAGATCCGTTACCCGAAGTGGCCTGCACCAAGCAAGGCGGTTGTGCCGC *F TGGAACTTGCCACCCCTCAGCAAT *F ATGCGAGGTCACTCCGGAAGGTCCTGTCTGCAAGTGTCCTCCGCTATTTGTGGGCGACGCGAAGTCAGGTGGCTGTCGG *F CCAGATGGTCAGTGCCCGAATGGA *F GACGCCGATTGCCCAGCCAATACCATCTGCGCCGGAGGAGTATGCCAAAATCCCTGCGACAACGCTTGCGGATCGAATG *F CTGAGTGCAAGGTTATCAATAGGA *F AACCCGTCTGCAGTTGTCCCCTGCGATTCCAGCCTATCTCTGACACTGCCAAGGATGGATGTGCCCGGACCATATCTAA *F ATGTCTCACTGATGTCGACTGCGG *F AGGAGCGCTGTGCTACAATGGACAATGTCGTATTGCTTGCAGGAACAGCCAAGATTGCTCTGATGGCGAGAGCTGCTTG *F AAGAACGTCTGTGTGGTTGCCTGT *F CTGGATCACAGTCAGTGCGCTAGTGGACTGGCCTGTGTCGAGGGCCACTGCACCATCGGATGTCGCAGCAACAAGGAGT *F GCAAGCAGGATCAGTCTTGCATTG *F AGAACAAGTGCCTGAATCCCTGCCAGTCTGCCAACAGTTGTGGTCCAAATGCCCTGTGCAGCATTGATCAGCACCATAG *F CCAGTGCAGCTGCCCGGAAGGTTT *F CGAGGGCAACCCCACTCCAGAACAGGGATGCGTGCGAGTTCCGGCTCCCTGCTTAGCCAGTAATCAATGTCCCAGCGGA *F CACATGTGCATTGGAAATCAGTGT *F AATCTTCCCTGCACCAAAACAGCTTCCTGTGCAGTTGGAGAACGGTGCTATCAACAAGTCTGCCGCAAGGTGTGCTATA *F CCAGCAACAACTGTTTGGCCGGAG *F AGATCTGTAATTCGGACAGGACGTGCCAACCAGGATGTGATTCAGATGCCGATTGTCCACCCACCGAATTGTGTTTGAC *F AGGAAAGTGCAAGTGCGCCACCGG *F ATTTATAGGAACACCTTTCGGTTGCTCGGATATTGATGAATGCACGGAACAACCCTGCCATGCCAGTGCTCGATGTGAG *F AACCTCCCGGGAACCTATCGTTGT *F GTATGCCCCGAAGGTACTGTCGGCGATGGGTACTCCCAGCCGGGTTGTTCGCAGCCCAGGCAATGCCACAAACCAGACG *F ACTGTGCCAATAATCTGGCCTGTA *F TACATGGAAAGTGCACGGATCCCTGCCTACATACTGTTTGTGGTATTAATGCCAATTGCCAGTCGGAAGGACACGAAGC *F TCTGTGCAGCTGTCCAGCTGGATT *F CTTGGGAGACCCCAACGACACTGGAGTCGGATGCTTTAAGGTGGAGTGCATCGACCATGTGGATTGCGCCGGAGATCGT *F GCCTGCGATGCGGAAACCAATCGA *F TGTATTAAACCATGTGACCTGACCAGTTGTGGAAAGGGCAATTGCCAGGTGAGGGATCATAAGGCGACATGTGCCTGCT *F ACGAAGGATATCAATTAGTGAACG *F ATGTATGTGAGGACATTAACGAGTGTCTGTCGCAGCCATGCCACAGCACAGCTTTTTGCAACAACCTTCCGGGAAGCTA *F CAGTTGCCAGTGCCCGGAGGGATT *F GATCGGAGATCCCCTGCAGGCCGGTTGTCGGGATCCCAATGAGTGTTTGAGTGATGCGGATTGTCCTGCCTCAGCTAGT *F TGCCAGAATTCCCGATGCCGTAGC *F CCTTGCGAGCGACAAAATGCTTGTGGATTAAATGCTAACTGCCAGGCACAGGCCCATCAAGCCATTTGCACTTGCCCTC *F TCAATTCCCGTGGAGATCCAACCA *F TCGAGTGCGTGCACATTGAGTGCGCCGACAACGACGATTGCTCCGGGGAGAAAGCATGTTTGGACTCGAAGTGCATAGA *F TCCATGTTCTCTGCCCAACGCCTG *F TGGAGCTTTGGCTCGCTGCTCGGTGCAGAACCACATCGGCGTCTGCTCCTGCGAAGCGGGCAGTACCGGAGATGCCAAA *F TTGGGCTGTGTGCAACTGCAATAT *F TGCCAGCAGGACGGACAATGTGCCCAGGGAAGCATATGCTCCCATGGAATTTGCAGTCCGCTGTGCAGCACCAACCGCG *F ATTGTATTTCGGAACAGTTGTGCT *F TGCAGGGCGTCTGTCAGGGAACCTGCAAGTCAAACTCAAGCTGTCCCCAATTCCAATTCTGTTCGAATAACATTTGCAC *F CAAGGAGCTGGAGTGCCGATCCGA *F TAGCGAATGTGGCGAAGACGAAACCTGTCTGAGTGATGCCTATGGGCGTGCAAAGTGTGAATCCGTTTGTCTTGGTCGC *F GCTGCCTGTGGTAGGAATGCAGAA *F TGTGTAGCCCGTTCCCATGCTCCCGATTGCCTCTGCAAGGAGGGATTCTTCGGGGACGCCAAGTCTGGATGCCGAAAGA *F TCGAGTGCACCAGCGACGATGACT *F GCTCCAATGACAAGAGCTGTGACAACCACATGTGCAAGATTGCCTGCCTAATTGGACAGCCGTGCGGAGAGAATGCCCT *F CTGCACCACAGAACACCACCAGCA *F GGTTTGCCACTGCCAGCCAGGATTCTCTGGAGATCCGCGGGTTCGTTGCGATGTTATAGACTTTTGCCGGGATGCTCCG *F TGCGGACCCGGAGCCCGTTGCCGA *F AACGCGAGGGGGTCGTACAAGTGCACCTGTCCACCGGGACTCGTTGGTGATCCGTACAACGAGGGCTGTCGCAGCTCCG *F TTGAGTGCGAGACCAACGAGGACT *F GTCCCCCGCATGCCGCATGCACCAAAACCAATGGGGTGGCCAAGTGCCGCGATGTCTGTGCCCAGCTGCAATGTGGCCC *F CAATGCGGAATGTGTTCCGAAGGG *F CCACGTGGCGCAGTGTGCATGCCGCAGCGGCTACGATGGCCAACCCGCCGACCGGGTGGCCGGCTGTAAGCCGCTGCCC *F TCTCCCTGTCAGGTCACCGGCGAC *F TGTCCGACCAACACATACTGCTCGGATAGCGTCTGCAAAC|CTGCCTGTGTTCTGGACACCGAATGTGGAGCATTTGAA *F GTGTGTCAAGGTGGCCAATGCTTCA *F ATCCGTGCCTTCAACCTCAAGCTTGTGGCCAGAATGCCGAGTGCGTGATGCAGAATCATCTGAAACAGTGCCATTGCCC *F GGAGGGCTTCACCGGCGACTCAGC *F GAAGGAGTGCGTGCGTGTGCCAGTGGCCTGTGATGGCGAGTGTGGTCCAGGATATACCTGCCGCGACTCCATGTGCCTG *F CCTGTTTGCCACAACGATCTGGAG *F TGCGCCTCCAACGAAAAGTGCCTGAAGGGCAGCTGTATGC|TGACCTGTCGGGTGGACAACGATTGCTTCCTGGGTCAC *F GTTTGCCTGCACAACAAGTGCGTGT *F ATGGCTGCCATGTGGACGATGACTGCAGTGCGTCAGAGTCTTGTCGCAACGATAAGTGCGTAAACCCCTGTCTGGAAAA *F TCCCTGTGGTCCCAATGCCGCCTG *F TTCGGTGTCCAACCACCGGGCTAGCTGCAGTTGTTTGGAGAGTATGGTGCCCAATCCCACACCCCAGGTGGGCTGCGTC *F CGTTCTCCCCCCTTGGAATGCCGC *F GAGAATCGCGACTGTGGAAATGGATTGGCTTGCTTCGAGTCGGTATGTCGACCACTATGCGCCGACGATGCTGGCTGCT *F TGACCAACGAGCGATGCCAGCAGG *F GAGTTTGCAAACCCCTGTGCCGCCACGACAATGAGTGCGGTCATGGAGAGCTCTGTCTGGGCCTGAACTGCGTACCTGG *F ATGCCGGTCCGATCAGGGCTGCCC *F GCCGGAATTGTCCTGCGTGGGTCAGCAGTGCGTGGATCCATGTGCAGATCCCACTGCCTGCGGCACCAATGCCCACTGT *F CAAACGATCGATCACAGGAAACAG *F TGCCTATGTCCAGAGGGTCTTGATGGCAATGCCAATGTGGCCTGCAAGGTGCCTCGCATTGCTTGTGGCAGGAACGAGG *F ATTGCCAGTCGAATCAGCTATGCT *F ATGCCGGCAGCTGCCAAGGAAAGTGCAGAAACGACCAGAACTGCTTGGCCGATGAGCGTTGCATGAGAGGAACATGTAG *F AACCGTATGCAACACCGATGAGGC *F ATGTGCTCAGGGACAGATTTGCGAGAATCGAATGTGCCAGACTGGATGTCGTACCGATTTGAGTTGTGCCACCGACGAG *F GCCTGCGTGAACAAGAAGTGCCAG *F AATCCATGTCGTACACCCGGCCAATGTGGCCAGTGTGCCGACTGCCTTGTGGTCAACCATGGAGTCCAGTGCCAGTGTC *F CCGCCGCCTTTATGGGAGACGGTT *F TGACAGGCTGTCAACTTCCGCCGGAACGCTGTCATCCTGACTGTGAATGCGACGAAAATGGAGCCTACTGTGCCCCCAA *F GTGCTCGAGAACCGAGGATTGCGC *F CTGTGGACAACAGTGCGCCAGAGGAAAGTGCCGGAATAAGTGCGGACCCAAGCGCCAGTGTACGGTGGGTCAGCTTTGC *F GAGAGAGGCGCATGCATTGCCGGG *F TGCAAGTCCAACGGTGATTGTGCCGCCGACCAGAGTTGTGTCAATGGAAAGTGCTCCGATCCGTGTGCGAACGAGAAGG *F CTTGCGGACGGAATGCTCTTTGCA *F CTGTGTCCGAGCACCGGATGCTCTGCTACTGCCCGGATGGATATGAAGGCGAGCCCAGCAAGGAGTGCGTGCAGTTCGA *F GTGCCGTGTGGACACCGATTGCGA *F CAGCAATAAGCGCTGCGATCAAGGAAAGTGCCGCAATCCTTGCCTGGAGTATGGAGCTTGTGGAACGAATGCTCAGTGT *F CGAGTTGTCGGTCGCAAGGCCCAA *F TGTTCATGTCCGCCGGACTTCTTTGGCAATCCGACTAGTGAGTGCCGACCGCTGGAAGGTGGATGCTCTAGCAAACCCT *F GCGGTGAGAACTCCAAGTGTACTG *F AAGTGCCCGGAGGCTATGAATGCGCTTGCATGGACGGCTGCATTGGAGATGCCCACCAGGGATGCCTTTGCGGCGGACC *F TCTAGTGAATGCCTGTCGCGATCA *F ACCGTGCGGTCTGAATGCCGCCTGCCACGTATTGGAAAACAATCAAGCCGAGTGCTACTGCCCGGAGGACTTCCCCAAT *F GGTGATGCTTATGTACAAT|GTTAC *F TTGACTACGCCGAAGCAGGATTGTCGTACCCTTGGATGCGAAGTCGGCGGCTGTGTACGCCAGGGCTATGAATACGTTT *F GCCAGCAGG|ACACCGAACAATGCT *F ACTCAGATACGGATTGTCCCAGTGAAAAGTCATGCCTCCAAGGACACTGCAGCGATCCTTGTACAATGCGTGGCGTGTG *F TGGTTTGAATGCTCTCTGCAAAAC *F CGTACTGCATCGACCGCGCTGCTCCTGCCCCAGTTGCCACATCGGTCGGCCAGAGATCGAGTGTAAGTCTGATCCCAAG *F TGCGTGGCCGAGGACACGGATCCA *F AAGACCAAGGAGCAGATCCCATGCTCCACAGACTCCGAGTGTCCAGAGACATTGCAGTGCGGCCAGTACGGCCAATGCA *F CAGATCCGTGCAACAATCCGCTCT *F TCATCTGCGAGAGCAACAAGAAGTGCGAGACGCGACGCCACCAGCCCGTTTGTATCTGCAAATCGGGCTTCATAGTGAA *F TGAGTACGGAGAACTGACGTGCGC *F CCCGGACAAGCGGGAGTGCTATCGCGACGATGACTGCGCCTCGAACATGGCCTGCTCCGATGGCAAGTGCCGCAATCCC *F TGCATCGTGCCCCTGGGCCGAGCC *F GCAATCTGTGCCGAGAACAAGTCCTGCGAGGTGCAGAACCACAAGCCAGTCTGCATTTGCATGCGCGACTGCCAGCCAT *F CCATATCGATTTGCCTGCGCGACG *F CCGGCTGTCCGGCTAGCCAGGCTTGCCGGAAGCTCAAGTGCGTCGATCCGTGCGAGTTTGCCACATGCGCACCCAACTC *F GCCATGCATTGTCGAGGATCACAA *F GCCGATATGTAAATTCTGCCCAGCTGGATTTATAGCCGATGCCAAGAATGGATGTCAAAAAG|CTAAGCCAGGTGGAAA *F CTGTACGTCAAACACAGATTGCAGT *F CAAGCGCACCAATGTGGATCGTCGGGAAAATGTATCGACCCCTGCCTCACTTCCTGTGCAGGCGGAGTAAAGTGTGTTG *F TATCAGCACATAGGGTTACTATAT *F GTACTTGTCCAGCCACCTTAACAAACAACACAGATTCGAACTGCACCTCGACAGACATAACAGTCGGGACTACAACGCA *F AAGAATTGAAACTACAACAGACTT *F CATTAACGTAAAATACACTGTAATGCAATTGGCAAATCAAACTGAAATGAGAACCCGCTTTACCGATATAGAAGCCGAA *F AACGAAACAACAGGTCCTTACACA *F ACCACAACAGAGTCCTATAAGACGACTAAGCAACTTTCTTCTAACCCAGAAACAGAGACACCCACAACTTTACCTTCAC *F GCCCAACAACTAGACCATTTACTG *F ATCAAACGACTGAATTTACTAGTGAAATTCCCACTATCACACCAATGGAGGGATCTACTCCCACACCATCACATCTAGA *F AACCACTGTCGCATCAATCACCTC *F AGAATCCACTACCAGAGAAGTATATACCATCAAGCCCTTCGATAGATCTACTCCCACTCCAGTATCGCCGGACACTACT *F GTTCCATCAATCACTTTTGAAACC *F ACCACAAATATTCCGATTGGAACAACACGAGGGCAAGTGACAGAACAGACTACATCTTCTCCCAGCGAAAAGAGAACTA *F CAATACGTGTTGAAGAATCTACAC *F TTCCTTCTAGGTCAACGGATAGAACCACTCCATCCGAAAGTCCTGAGACACCCACAATATTACCTTCAGACTCCACTAC *F TCGAACTTACTCAGATCAAACGAC *F TGAATCTACTAGAGACGTTCCAACCACCCGGCCATTTGAGGCATCTACTCCCAGTCCAGCATCTCTGGAAACAACTGTT *F CCATCAGTTACTTTAGAAACCACT *F ACAAATGATCCAATCGGTTCAACAGGTGGGCAAGTGACAGAACAAACTACATCTTCTCCGAGCGAAGTCAGAACTACAA *F TAGGTCTTGAAGAATCTACACTTC *F CTTCAAGGTCAACGGATAGAACTACTCCATCCGAAAGTCCTGAGACACCCACAACATTACCTTCAGACTTCATAACTAG *F ACCTCACTCAGATCAAACGA *F 3'half of dumpy: *F ACAACTGAATCTACTAGAGACGTTCTTACCACCCGTCCATTTGAGACATCTACTCCCAGTCCAGTATCTCTGGAAACTA *F CCGTTCCATCAGTCACTTCAGAAA *F CCTCTACAAATGTTCCAATTGGTTCAACAGGTGGGCAAGTGACAGAACAGACTACTGCACCTCCGAGCGTCAGAACAAC *F TGAAACTATAGTAAAATCTACTCA *F TCCTGCAGTTTCACCCGATACAACTATTCCATCCGAAATTCCAGCCACGAGAGTACCTTTAGAGTCAACTACTCGGCTA *F TATACTGACCAAACAATTCCACCT *F GGATCAACGGACAGAACAACTTCATCCGAAAGACCGGACGAAAGCACTAGATTAACCAGTGAAGAATCAACAGAAACTA *F CTAGGCCAGTACCAACTGTGAGCC *F CAAGGGATGCATTAGAGACGACAGTTACTTCATTAATCACAGAAACAACCAAAACTACTTCTGGTGGTACACCACGAGG *F ACAAGTGACAGAACGGACTACCAA *F ATCGGTCAGCGAATTAACGACTGGGCGCTCAAGTGACGTAGTGACAGAGCGTACTATGCCAAGTAATATTTCGTCAACT *F ACGACTGTTTTCAACAATTCGGAA *F CCCGTTTCGGATAATTTGCCAACTACAATAAGCATTACAGTGACAGATAGCCCAACGACAGTACCTGTACCGACTTGTA *F AAACAGACTATGACTGCTTGGACG *F AACAGACCTGCATTGGCGGACAGTGCATATCGCCCTGTGAATACTTCACTAATCTCTGCACGGTGCAGAATCTGACCAT *F TTGTCGTACATTGAACCACACCAC *F AAAGTGCTACTGTGATACCGACGATGATGTAAATAGACCCGATTGCTCTATGAAAGCAGAG|ATTGGATGCGCATCGAG *F TGATGAGTGTCCGTCCCAGCAGGCG *F TGCATTAATGCTCTTTGTGTGGATCCTTGCACCTTTAACAATCCCTGCAGCCGTAATGAAGATTGCCGAGTTTTTAACC *F ACCAGCCATTGTGCTCAGCCG|AGC *F ATGGTCGCACGCCTGGATGCGAGCATTGTCCACCAGGCGCGAACTGCGATCCAACAACCGGTGCTTGTATAAAGGGTAA *F GTTCTACTGTCAGTCCTTCTCCTT *F AATCAACCTAAAACTCTTAGCTTTAAGTGTAAATACAATGTGTACATATTCTAATTTAAAATTAACCTCCCGTTACACC *F CGTGTAAATACATCTCTCTCCTTA *F GCTAATGTAACAATAACAACTATTACTACCAAGAACTCCACATCTACCAAGATACCAACGAAACCAAGAACAACAGCTA *F ACCCAAACACAGGCGTCAAAACGA *F CGCCGACAACCACAAGGGTCACCACAAGAAATACCACCACGACCACAACCACTACTACCACTTCCTCCACTAGCACTGA *F ATCGAGCACAATCACAAGCGCTAC *F TAACCAGACCAGTAAGAACCAAAAACCAGACACTGAAAGCACCACCTCCCACACCGATGCCACGAGGCGGTATCGCGAT *F GGTGAGAACAATATCACCGATACT *F CCGACACCGAGGCCCACGATCCAGACGACCACGCTGCGGGGCGAAGGTGTCATGGGTGACAGTCAGAGGCGGTCCACTA *F CGACGCCCAAAATGAAGACCACCC *F GGCTGGATACTTCCAACGAGGTACCAGATACCACATCGCCGTGGCCAATAGAGCTGCCCACAACGGAGGGCACCACCAC *F GGAAGTCTACAACACCATGTTTGC *F CCCGGTGGTCAACACCACTGACACCTCATTAATTAACCCATGTACAGTAGATACTAATTGTGCCCCTAACGAGCACTGC *F AAACTGGGCCATTGCCGTAAAAAA *F G|AACCGCCGGGTTCACCGAAAACACCCGAACCCTGCCAATCTAACAACGACTGTATTGAGAGCGAGGCCTGTTACATG *F GGTTTGTGCCAGGATCCTTGTGAGT *F TTGCCAAGATCTGTGCCGCGACTGCCAAGTGCACGGCCAAGAGTCATCGACCAGTGTGCACCTGTCCGCAGGGCCATGA *F GGGCAATCCGATGGTCAAATGTGT *F AACCACTCAAACCTCAA|TTGAATGCACTGACGATTCGGACTGCGGTGTCACGGAAGCGTGCATCAACCAACTTTGCCA *F GCATCCTTGCGATGTTCACGATCCT *F TGTGCCACGAATGCTGTTTGCATCAACAGCAACCACGCAGCTGACTGCAGTTGCGCGGATGGCTTCCAGGGCAATGGAT *F TTGTTGGCTGTCAGCCAG|CACGTT *F CCCATGTCTGCCAATACAACGAGGACTGTCCACCGACGAAGCTCTGCGATCGCCTAAACCGACGCTGCATCAATCCCTG *F CCAAGAGGACTCTTGCGGCGAGAA *F TGCCGAGTGCATACCCGTGAACCATGGCACTGAATGTCGCTGCCTGCCCGGATTTCTGGGCAACGCCTACGTGCAGTGT *F CTCCCTAGTCAGGGATGCCGCTCC *F GATTCCGAATGTGATTCGAGTCAGGCCTGCATCAACGGAAAGTGCTCATCGCCCTGCCAGTGTGGAGCCTATGCCCTCT *F GCGATGTGGTTAATCATCGTGGTG *F TTTGCAAATGCCCTCCCGGCTACAACGGAAATCCCAAGGTGGGCTGCAGTCCGCCTCAAGATCCATGCGACCCCAATCC *F ATGTGGCTTAAATGCCTTGTGCGA *F ACTGGACAATGGCAACCCCATCTGCTACTGCCCGAAGGGTCTTACCGGAAATCCGTTCAAAAATTGCA|TTCCTGAGGG *F AGATGAGTGCACACCGAATCCCTGT *F GGTCCCAACTCTGGATGTCGTCGCGTAGGTGGAAACCCGGTGTGCTTCTGTCTGCCGGAATATGAGGGTCAACCACCGT *F CGATTCCTTGTGAGCTACCTTCCA *F ATCCCTGCGACCCATCGCCTTGCGGACCGAATACTCAGTGCTCTGTGCTCAGCAATGGGTTCTCCAAGTGCACTTGTCT *F GCCGAACTATGTGGAGAGTCCGAA *F CACCATCCGTGGATGTGTTGAACCTATTAACCCGTGTGACCCGAACCCTTGTGGAACTGGTGCAATCTGCGATTCATCA *F CGTCACCCCGTGTGTTACTGCCCG *F GACAACAAGATTGGCAATCCTTTCAGACTGTGCGACAAACCTGCAGTTACCATTGAACTTTGCCAGCCAGGACCATGTG *F GTCGAAATGCCGAGTGCTATGTGG *F CTGGAAATCGCGAGGAGTGCTACTGCCGCTCGGGATATGTGGGAGATGCTTACCAGGGATGCCGTGAACCGAGTCGCAC *F CGTTTGCGACCCCAATCCTTGTGG *F ACCCAATGCCAACTGCGTTGTGGCTGGCGACGGACAAACGGCATGCGTTTGTCCTGACGGTTTGTCAGGAGATCCCACG *F TCCGTAATTGGCTGTCATGGCTAC *F GAATGTCAGGTGGATGCCGATTGTCCAAATAGCAAGGCCTGCATGGGCTACCGCTGCTACGATCCCTGTCCAGGAGCAT *F GTGGCCAAGGAGCTCACTGCCAAG *F TGGAGGAACATCACCCAGTTTGCTCTTGTAACTCCGGCTTGACTGGTAACCCTGGAATTCGCTGCTATGCTTTGGACCA *F TCCCAAAAAGAATCCTTGCGTTCC *F TAGCCCTTGTGGCCGAAATAGCGAGTGCAAGTTGCTAAATAACCGCGCTGTTTGTTCGTGCATCCCAGGTTACTTGGGC *F GATCCTCAATCGGGATGCCAACCT *F GAGTGCGACATTAACTCCGATTGCGGTGATACCTTGTCGTGCATTAACCACAAGTGCGTGGATCCATGTGCCGGAGCGA *F TCTGTGGAATTAATGCCATCTGTA *F ATGTGCGCCAGCATACGCCAGTATGTCTTTGCCTGGACGGCTTCGTCGGCGACGCCTTTTTGCAGTGCGTTCCCATTGG *F AATACTGAAGAACGTTTCTCGGGA *F TCCGTGTGCTCCGTCGCCCTGTGGACCTCATGACGTTTGTTCGGTGTACGGCGATGGAGTGGCCCTTTGTGATCCCTGC *F TTTGGGCCAAATGCCCAGCAGAAT *F CCACGCTGCCGACCCGAGTGCGTCGGCAATTCGGATTGTCCGTTTGATCGCGCCTGTTTGGGTCAGCGTTGTCTGGATC *F CCTGTCCCGGATCCTGTGGCCGGA *F ATGCTATCTGTAATGTCTACGAACATAATCCCGTATGCGCTTGTCCCACAGGACTATTTGGCAATCCGTACGAACAGTG *F CACAACTAAGTCGGTGGTCGAGAC *F GCCACCTCAACCCAGCTGTGCTAAACTCCATTGCGGTGCCAATGCGGAGTGCAAGCGTCAGCACAGCGGTCTGGCCTGC *F GTCTGTCGTAAGGGTTACTTCGGA *F GACCCTCATATTGGTTGTCGGCCAGAATGTGTTCTTAACAGTGACTGTCCCGCTGAAAAGGCTTGCTTGAACTCCAAGT *F GCGTGGAAGCGTGTACCGGTGTGT *F GTGGCGTTAATGCAGTTTGCCGTGTGGTTAACCATGCGCCTGTTTGCATATGCGCCGAGGGATATAGTGGAGATGCCTC *F TATTGCCTGCAATCCGTTCTACCT *F GCCACCTCCAGAGCGACCTCATCCGTGTGAGCCATCACCATGCGGACCAAACTCCCGATGCAAGGCCACCCCTGATGGC *F TATGCAGCCTGTTCCTGCCTGCCG *F AACTTCAAAGGAGCTCCACCAGTCTGTCAGCCAGAGTGTGTGGTTAGCTCGGAGTGTGCTCCCAACCAGGCATGTCTGA *F ATCAGAGGTGCACAGATCCATGCC *F CAGGTATTTGCGGTGGTGGAGCTCGCTGCGAAGTGCTGAACCATAACCCCATCTGCTCCTGTGAGGCCAACTTCGAGGG *F TGATCCGTTTGTGGCGTGCTCACC *F CATTCAGGATCCAGGTCGCGACATCCCTGTGCCAAAGAACCCGTGCGTGCCATCTCCTTGCGGACCCAACTCCATTTGC *F CAGATTAAACAAAACCGACCAGTC *F TGCTCCTGTGTGGCCAACTATATCGGCAGCCCACCGTATTGCCGACCGGAGTGCACGCTGAGCAGTGAGTGCCCATCGG *F ACAAGGCCTGCATCAACGAAAAGT *F GCCAGAATCCGTGTGCCAACGTATGTGGACACAATGCCCGTTGTACGGTCATCGCTCACTCCGCTCACTGCAGTTGTGA *F CGAGGACTACGAGGGAGATGCCTT *F CATTGGTTGCTCTAAGAAGATTACAGAAA|GGCCCGGTGATCACATTGATCCATGCTACCCTAACCCATGTGCCGAGAA *F CGCTGTTTGCACGCCATACAATAAT *F GCCGCACGCTGTACCTGCATTGAGCCCTATAATGGCGATCCTTACAGTACCGGATGTCGACCGGAATGTATCTACAGCT *F CGGAGTGCCCCTCATCGCTGGCCT *F GCATCAAGCAGCACTGTCGCGATCCATGCACGGCTGCTTGTGGAGCGAATGCCGAATGTACGGTGGTCAACCACTTACC *F CTCTTGCAGTTGCACCAGAGGATT *F TGAGGGTAATCCGTTTGACGGATGCAAACGGGTGGTCGTTGTGCGACCCGAAACCGTGTGTGAACCCAATCCCTGTGGA *F CCGAACAGCATTTGTCGCTCTGTA *F GAGGGACATCCAACGTGCAGTTGCCAGGTCGGTTACTTCGGAGCTCCGCCACAGTGCAGACCCGAATGTGTGGTCAGCT *F CGGAGTGTGCTCAGCATTTGTCCT *F GCATAAATCAGAAGTGTATGGATCCGTGTGTGGGCACTTGTGGATTCAACGCCAAATGTCAAGTCAACAACCACAATCC *F TATTTGCTCCTGTCCAGCCAACTA *F TGAGGGAAATCCATTTGAGCAATGCATGCCCAAAC|CCGCAGAACCAACTCGCAACGTAGATCCATGCCTTCCCAGCCC *F ATGCGGTTCCAATTCCATTTGCCGA *F AATGTGAACAATAGGGCAGAGTGCTCTTGTGCGCCAGGAATGTTTGGAGCACCACCGAACTGTCGACCTGAATGTGTGA *F TTAACCAGGATTGCCCATCAAATC *F GAGCTTGTATACGGCAGCGGTGTGAGGATCCTTGCATTGGCATTTGTGGCTTCAATGCCGTGTGCTCCACGCAGAACCA *F CCAGCCCAAGTGCAGTTGCATTGA *F AAGCTTTGAGGGTGATCCGTACACCGCCTGCAAAATGCGTGAGA|TCGTGGTGCTAGATCCACCCACCGATCCATGCTA *F TCCATCGCCATGTGGTGCGAATGCC *F ATTTGTCGCGTGCGCAACGGAGCCGGCTCCTGTTCCTGCATCCAAAATTATTTCGGTGATCCCTATATCAACTGCCGCC *F CTGAGTGCGTCCAGAATAGCGATT *F GTCCCAATAACCGTGCCTGTATAAACATGAAGTGCCGCGATCCCTGCGCCAATGCCTGTGGATTTAATGCCATTTGCCG *F GGTGGCTCATCATCAGCCCGTCTG *F TAGTTGTGAACCCCACTTAACAGGCAATCCACTGCGCGCATGCGTCGAGAGGCCCTCAA|ATATGTACCTACCCCTGCC *F CAAAGACCCTTGTAGACCGTCGCCT *F TGTGGCCTGTTCAGCACTTGTCATGTAGTTGGGGAGCGTCCTGTGTGTGCCTGCTTGCCAGACTACATGGGTGCTCCGC *F CCAACTGCAAGCCCGAGTGTATGA *F CCAGTGCCGAGTGTCCTTCGGATAGGGCCTGTATAAACCAGCGCTGCAAAGATCCTTGTCCCGGAACATGTGGCTACAA *F TGCCCGCTGTCGCTGCACGAATCA *F TTCACCAATATGCAGTTGCTACGATGGTTACACCGGGGATCCGTTCCACCAGTGCGTTCCTGAAAGAA|AACCACCACC *F AATAGCTGATCCCATTGTTCCGCCC *F AATCCTTGTGTGCCATCACCTTGCGGACCCAACTCTCAGTGCCAGGTTTCAAGCAGTGGAGCAGTGTGCTCCTGCGTAA *F CCAATTACATAGGACGACCACCTG *F GTTGTCGGCCAGAGTGCAGTATTAATTCCGAGTGTCCGGCCAGAATGGCCTGCATCAATGCTCGTTGTGCCGATCCGTG *F CATCGGATCGTGTGGAAATAACGC *F ACTCTGCCATGTCAGTCTGCATGCACCGGTGTGCATGTGTGAGCCCGGATATTCCGGCGATCCGTTCTCCGGCTGCTAC *F AAGATAATTGAGA|CCCCGATTGAG *F GTAATCCAACCGTGCCGTCCCAGCCCATGCGGCTTAAATGCCCTTTGCGAGGAGCGGAATCAAGCCGCCGCCTGCAAGT *F GTCTTCCGGAATACTTTGGGGATC *F CATATGTCGAGTGTCGCCCCGAGTGCGTCATCAACTCGGACTGTCCCAGGTCGCGAGCCTGTGTCAATCAAAAGTGCGT *F GGATCCCTGTCCGGGAATGTGTGG *F TCACAACGCTCTATGCGCCGTCTTCAACCACGCTCCCAACTGCGAGTGTCTTCCTGGTTATACGGGCAACCCCATCGTT *F GGTTGTCACATAGTCCCTGAAAGT *F CCGCGCT|ATCCCGACCCAATAGTGCCTGAAAACCCTTGCCAACCCTCGCCCTGTGGCCTCTACAGCAACTGTCGCCCG *F GTCAATGGCCACGCAGTTTGCTCCT *F GTGTGCCCAGCTACATCGGCTCTCCTCCCAACTGCCGGCCAGAGTGCATGAGCAGCTCGGAGTGTGCGCAGGACAAGTC *F GTGCCTTAACGAGCGCTGCAAGGA *F TCCCTGCCCAGGCACCTGCGGCAATAACGCGCTCTGCCGCGTAGTCAACCACAACCCCATCTGCTCCTGCAGTCCCGGC *F TTCAGCGGTGATCCCTTCGTGCGC *F TGCTTCCCACAAGAGA|AGCGACCGCCCATTACCCATGACCGCATCGATCCGTGCGTGCCCTCGCCATGTGGGCCCAAC *F TCTGAGTGCCGGGTGTCGGCCGCCA *F ATGAGCAGGCTGTCTGCTCATGCTTGCAACATTACGTGGGTAGGGCGCCAAATTGCCGACCGGAGTGTACATCCGATTC *F CGAGTGCCCTGGCAATTTGGCGTG *F CATAAATCTGCGCTGCCGGGATCCGTGTGTCGGTACCTGCGGCATACAGACCACCTGCCTTGTAAATAATCATAGACCC *F ATTTGCCGCTGCATTGACGGTTAT *F GCAGGCGATCCATTCTCAGAGTGTAGTCCAAAGA|TTAATGTCCCGGTACAAGTGGCACAGCCCTGCAACCCGAGTCCC *F TGTGGCGCCAATGCTGTGTGCAAAG *F AGCGCAATGGAGTGGGTTCCTGCTCCTGTCTACCGGAGTATAATGGAGACCCGTACACGGAGTGCCGCCCAGAGTGCGT *F GCTCAATAGTGACTGCTCGAAGAA *F CCGCGCCTGCCTCAACAACAAGTGCCGCGATCCCTGTCCGGGAGTCTGTGGCGTCTCGGCCGAGTGCCACGTGATCAAC *F CACGCCCCCAGCTGCAGTTGTCCT *F TCCGGTTTCACTGGAAATCCCTCGCAATTTTGTCGGGAGATACCAAGAT|TGCCCGCTCCCGTTGAACCATGTCGTCCT *F TCGCCCTGCGGACCCTACAGCCAGT *F GCCGTGAGGTTAACGGACATGCGGTCTGCTCCTGCGTTACCAATTATATTGGCACACCGCCCGCTTGTCGTCCGGAGTG *F CTCGGTTAGCTCCGAATGCGCTCA *F GGACAGAGCATGTGTGAACCAGCGCTGTGCGGACCCATGTCCTGGTACCTGTGGCAATGAGGCTATCTGCAAAGTGACC *F AACCACAACCCGATCTGCTCCTGC *F CCAGCCGGCTACAGCGGCGATCCTTTCGTTCGCTGTGCGCCGTGGCAGGAGGAACCGGAGCAACCCAAGTCCAATGAAA *F ATCCTTGTGTACCCAGTCCCTGTG *F GACGCAATTCCCAGTGTCGTGTCGTGGGCGAGACGGGCGTGTGCTCCTGCCTGCCAAATTTCGTGGGTAGGGCGCCAAA *F TTGCCGCCCAGAGTGCACTATCAA *F CACAGAGTGTCCTGCCAACCTGGCGTGCATCAATGAGCGCTGCCAGGATCCCTGTCCGGGATCGTGTGGCTTTAATGCT *F TTCTGTAGTGTTGTAAACCACTCG *F CCCATCTGCACCTGCGACTCTGGTTACACAGGTGACCCATTCGCCGGCTGTAACCCACAAC|CTCCAGCCATACCCGAT *F GAACGTCTGACTCCCTGCCAACCCT *F CCCCCTGTGGTCCCAATGCCGAGTGCCGTGAGCGCAACGGAGCCGGCTCCTGTACTTGTCTGCCGGAATATTTCGGTGA *F TCCGTACAGTGGCTGCCGTCCGGA *F ATGCGTGGTGAACAGTGATTGCTCGCGCGACAAGTCGTGTGTCAACCAGAAATGCGTGGACCCGTGTCCAGGTGTTTGT *F GGCTTGAACGCCCAGTGTCGTGTG *F TCCAATCATCTTCCCAGTTGCTCATGCTTGGCTGGATACACCGGAAATCCGTCGAGCGCGTGCCGTGAAATTCCCCAGT *F TGCCCCCACCAC|CCGAACGGGATG *F AGAACCCCTGTAGACCATCTCCCTGTGGTCCCTATAGTCAGTGCCGCGAGGTCGACGGCCATGCCGTGTGCTCCTGCCT *F TCAGGGCTTCATCGGATCAGCTCC *F CAACTGCCGGCCCGAGTGCATCATTAGCTCGGATTGCGCCCAAAATCTTAACTGTCAGAATCAAAAGTGCGTGGACCCG *F TGTCCGGGCACTTGTGGCATCGAG *F GCGCGCTGCCAGGTCATTAACCACTATCCCGCATGCTCCTGTGCCCCAGGTTTTACCGGAGATCCCTTCAACCGGTGCA *F CCAAGATATTGT|TGGAGCCCCCAC *F CCACCGAAAAGTCTGGTAATCCCTGCATCCCATCACCATGCGGACCCAACTCGAAATGCCTCGATGTCCGTGGTTCGCC *F TGCATGCTCCTGTTTGCCCGACTA *F TCTGGGACGTCCGCCAAACTGCCGGCCCGAGTGCCTCAGTAGTGCCGACTGTCCGGCTAACCTGGCCTGTGTGAACCAG *F CGCTGCTCGAACCCATGTATCGGC *F GCTTGCGGCCTGCACTCCGTGTGCACGGTCATCAAGCACCGACCAGCATGCGAGTGTGTGCCAGGTTACACCGGAGATC *F CCTTCTCAGGCTGTGCGATCGTAC *F AGCAAA|TCGCCCCGCCGGACGAGACAAGAAATCCCTGCAATCCTAGCCCCTGCGGAGCAAATGCCATTTGCCGGGAGC *F GCAATGGAGCTGGCTCTTGTGCCTG *F CCTGCCCGAGTACTTTGGTGATCCGTACAGTGGTTGCCGACCGGAATGTGTCCAGAACGACGACTGCGACCGCTCGCGT *F GCCTGTATCAACAACAAGTGCCAG *F GATCCTTGTCCCGGAGCCTGCGGCATCAACGCTGAGTGCAGAGTTTTGAATCACGGACCGAACTGCAACTGTTTCGATG *F GCTATACTGGAGATCCGCATCGCT *F CGTGCTCGTTGATCGAGGTGGTCACTATTCGACCGGAGCCATGCAAGCCATCACCCTGCGGACCGTACAGCCAGTGCTT *F GGACACCAACAGTCATGCAGTGTG *F CAGCTGCTTGGAAGGCTACATTGGTGCACCGCCCAGCTGCAAACCGGAATGTGTGGTCAGCTCGGAGTGCCCACAAAAT *F AGGGCTTGCATTAACCAGAAGTGC *F GAGGATCCTTGTCGAGGATCGTGTGGCAATAATGCTAAATGCCAAGTGGTCAACCACAATCCCATCTGTACCTGCCAGC *F CTGGCATGACAGGTGATCCGATAT *F CGGGATGTGAGCCCATGCCCGAAGTCAAGAACGTGGAGAATCCGTGTGTGCCGTCACCATGTGGACCCAACTCTGTTTG *F TCGACAGATTGGAAACCAGGCAGC *F ATGTTCCTGTAACGCTGGCTATATTGGACGCCCACCCACTTGCCGACCGGAATGCACAAACAATGACGAGTGCCAGAAC *F CATCTTTCCTGTCAGCAGGAGCGC *F TGCGTGGATCCGTGCCCCGGATCGTGTGGTAGCAACGCCATCTGCCAGGTGGTGCAGCACAATGCTGTATGCTCCTGCG *F CCGATGGATACGAGGGAGAGCCAC *F TCTTTGGTTGTCAGCTGATTCCAGCTGTGACGCCCACCGAGTCACCATCCTCGCCCTGCGAGCCATCACCATGCGGTCC *F TCATGCCGAGTGTCGTGAAAGGAA *F TGGAGCTGGAGCTTGCTATTGTCACGATGGATTCGAGGGTAATCCCTACGATGCCCAACGAGGATGCCGACGAGAGTGC *F GAGAATAACGATGACTGTACTGCC *F GTCCAAGCCTGCTCTCGATTCAAGTGCGTCGATCCCTGCAACAATATTTGCGGAGACTATGCCATTTGCACAGTCGACA *F AACATGTGCCCACTTGTGACTGCC *F CGCCTGGATACACTGGAGATCCGTTCTTCAGCTGCAAACCCGTGCCGGTCACACCGCGTCCTCCGCTCAACCCATGCAA *F CCCATCTCCATGTGGACCCAACAG *F CAATTGTCGGGCCATGAACAACCAGGCAGTGTGTTCCTGCCAGGCAGGATTCATCAACCAGCCACCCAACTGCAAACCG *F GAATGCGTGGTCAGTGCCGAATGT *F GCTCCGGAAAAAGCATGTGTACACAAGAAGTGCGTTGATCCCTGTCAGCATACCTGCGGTATCCGGGCCATCTGCACCA *F CCAAAAACCACAGCCCCATCTGCA *F CGTGTCCCCGAACAATGACTGGAGATCCCTTCGTTGAGTGCACTAGAGTTG|CAATCACCAATGACAATACGACACCGT *F CGCCGGCTCCAGCATCCTGTGTGCC *F TTCGCCTTGTGGACCCAATGCCAAGTGCCAGATCGTGGGCAATAGTCCGGCATGCTCCTGCCTGCCGAACTTCATCGGG *F GCTCCACCCCGCTGTCGCCCGGAG *F TGCGTCTTGAACTCGGAATGTGGACCCACAGAGGCGTGCATAAACCAAAAGTGCGCAGATCCATGCTCTGGATCGTGCG *F GATTTGAGGCCAAGTGCCATGTGC *F TCAATCACCTGCCCATTTGCAACTGCATTGAGGGTTACGAAGGTGATCCGTTCGTGCGTTGCACCAAGAAGGAAGAAGA *F TAGATCTCCTCCACCCCCGAATGA *F CCCGTGCAACCCGAACCCGTGCGGACAAAATGCGGATTGCTTTGCCGGAGAGTGCCGCTGCCAAAATAATTACCAAGGC *F AATGCCTACGAGGGATGTCGTCCT *F GAGTGCACGCTCTCAGCGGACTGTCCAAGGGACAAGGCCTGTATGCGAAACCGTTGTGTGGATCCCTGTCCAGGAATCT *F GCGGTAACAATGCTGTGTGCGAGG *F TGATGAACCACATACCGGTGTGCTCCTGCGTCAAGGGTTATGAAGGTGACCCGTTCGTCAACTGCCGCGTGAAACCCGT *F CGTGGAGGATCCTATTATTGAGGC *F CTGCTCCCCATCGCCATGCGGTTCCAACAGTCAGTGTCGCGACGTTAACGGCCATGCGGTTTGCTCTTGCCTGGAAGGT *F TATATCGGAGCACCACCCCAGTGC *F CGTCCCGAGTGTGTGGTGTCAAGCGAGTGCTCCGCCTTACAAGCCTGTGTCAACAAGAAGTGCGTAGATCCATGCGCCG *F CTGCTTGTGGTTTGGAGGCCCGCT *F GTGAGGTCATCAATCATAGTCCGATTTGTGGCTGCCCACCTGGCCGAACTGGAGATCCGTTCAAGCAATGCGTCGTCCT *F CCCACCGATTGCTGTTCCAGACGT *F AAAGTCACCTCCCCAAGATCCCTGTGTGCCATCCCCCTGCGGACCCAACTCGATCTGTAAGAATGATAGGAATGGCCCC *F GTCTGCCAATGTCAACCAGAGTTC *F TTTGGCTCGCCGCCCAACTGTCGACCCGAGTGTATCATTAATCCGGACTGTCAATCTACACAGGCGTGTATCAACAACA *F AATGCAGCAATCCATGCCCAGAAT *F CATGCGGAACCAATGCGGAGTGTCGTGTTATTGGACACGCTGTGTCCTGCTCTTGTCCAACAGGATACGCCGGCAACGC *F TTTCGTACAGTGTGTACCGCAGCA *F AGAGGAGCCACCGAAGCCGTGCCAGCCATCGCCATGTGGTCCAAATGCCGAGTGTATTGAACGGAACGGAGCCGCAGCC *F TGCAAATGTATTGATGAATACCAG *F GGTAATCCGTACGAGGGTTGTCGTCCCGAGTGCGTACTGAGCTCAGATTGTCCTACGGACAAGACGTGCATACGCAACA *F AGTGCCAGGATCCTTGCCCAGGAA *F TCTGTGGATTGAACGCCCAGTGCTATGCTGTGAACCACGTGCCCAACTGTGTTTGCAACGATGGCTATACTGGTGATCC *F GTTCGCCAGTTGTCGTCGAGTGGA *F GGTCACAACTCCATCACCAGTTTCGGATCCTTGCATTCCATCTCCTTGCGGAGCCAATAGCAAATGCAGGGTCGCAAAT *F GGCTTAGCCGTTTGCTCATGCATG *F GAAACATTCATTGGAGCCCCACCAAATTGCAAACCCGAGTGCACGGTTAATGCCGAGTGTCCTTCCAATAGGGCGTGCC *F ACAAGTTCCGTTGCGCGAATCCTT *F GCGCCAAGACGTGTGGCCTGAATGCCAAGTGTGAAGTGATCAACCATAATCCCATCTGTAGTTGCCCTCTGGACATGAC *F GGGTGATCCATTTGCTCGCTGCTA *F CCCAGCTCCCCCACCGCCGCCACCAGGACCCAAGGATGAGCCCGTTAGGAGACCATGTCAACCATCACCATGTGGATTG *F AACTCGGAATGCAGAGTACGTGAC *F GAGCAGGCCTCGTGCTCTTGCCTACCCAACTTTATAGGAGCACCACCTAACTGCCGACCCGAATGTGTTGTGAACACGG *F ATTGTTCGCCGGACCAAGCTTGCA *F TTGCCGAGAAGTGCCGTGATCCCTGCGATGGATCCTGCGGAGTCGACTCCGAATGCCGTGTTCAGAACCACTTGGCCAT *F CTGCACCTGCCGTGGAGGATTCAC *F CGGTGATCCGTTTGTTCGTTGCTTCGAGTTCGTCGAAGAGACCACAAAGTCACCGCCACTCACACAGGATCCTTGTGAT *F CTGCAGCCATGCGGATCGAATGCC *F GAGTGCCGAAACGGTATCTGTAGCTGCCTCGCGGACTACCAGGGAGATCCCTATACCGGCTGTCGTCCAGAATGTACAT *F TGAGTACTGATTGCGCACCTACCA *F AGGCTTGCTTAAACAAGAAGTGTGTTGATCCTTGCCCAGGAGTATGTGGGCAGAACTCGCAGTGTGATGTTTCAAACCA *F CATACCGATTTGCAGTTGCCTGCA *F GGGCTACACGGGCGATCCGTTTGTTCACTGCCGTCACGAGACGCCAGTGGCCAAGGATCCGTGCCAACCGAACCCATGC *F GGACCCAACAGCTTGTGCCACATA *F TCCGGACAAGGACCAGTCTGCGCCTGTCAACCGGGAATGCTGGGCTCACCGCCCGCCTGCAAACCGGAGTGCATTGTCA *F GCTCCGAGTGCTCGTTGCACACTG *F CCTGTGTGAACAGGAAGTGCGTGGATCCTTGCCCCGGAGCATGTGGGCAATTTGCTCGCTGCCAGGTGATCAACCACAA *F CCCGTCCTGCTCATGCAACACGGG *F ATACACTGGCGATCCGTTTACCCGATGCTATCAGGAGGAACGAAAACCACCGACGACGCCAGATAATCCTTGCCAGCCA *F TCTCCTTGCGGACCCAATTCCGAG *F TGCAAGGTGCTGAATGGAAACGCTGCCTGCTCGTGTGCGGCCACCTTCATTGGAACGCCACCAAGCTGCCGACCCGAAT *F GCTCCATCAATCCAGAATGTCCAC *F CAACCAAGGCCTGTATCCGCCAGAAGTGCTCGGATCCGTGTGTGAATGCCTGTGGATTTAATGCCCGCTGCAATGTGGC *F CAATCATCAACCCATCTGCACATG *F CGATGTGGGCTATACTGGAGATCCGTTTACGGGCTGTCAGAAGGAGCAAG|AACGCATTGTCAATGAGCAGGTGACTCC *F CTGCGAGCCCAATCCCTGTGGCTCG *F AATGCTGTGTGCCGCGAAAGAAATGGCATCGGTTCCTGCCAGTGCCTGCCGGATCATTTTGGTGACCCGTATCAGTCGT *F GTCGTCCGGAGTGCGTACGCCACT *F CGGACTGCGCCTCGAATAAGGCGTGCCAACAGCAGAAGTGTCGCGATCCCTGTCCAGGCACCTGCGGCAGCAATGCGGA *F CTGCAGTGTAACAAACCATCTGCC *F CACCTGTACATGCCGCATTGGATATACCGGTGATCCGTACCGCTACTGCCACGTGGAACCACCCCAGC|TCCCTGCCCG *F AGTGACCGAACCATCTCAACCCTGC *F CGCCCTTCACCATGTGGTCCCAACTCTCAGTGCCGCGAACTGAACGGTCAGGCCGTGTGCTCCTGCCTGGAGCTTTACA *F TCGGTCTGCCACCCAACTGCCGAC *F CGGAGTGTGTGCTGAGCACAGAGTGTCCCACCGATAAGGCTTGCATCAGTCAACGTTGTCAGGACCCGTGTCCGGGCAC *F TTGTGGCATCAATGCTGAGTGCCG *F GGTGCGTAATCATAGTCCTCTGTGTCAGTGTCGTCAAGGTTTTACCGGTGATTCCTTTACCCGTTGTTATCCCCTGCCA *F C|CCCCGCCTCCTGTAATCGAACGA *F GTTGAGCGTGATCCTTGCCTGCCATCGCCCTGCGGATTGAATAGCCAATGCCGCAATGTCCAAGGTGTGCCGTCCTGTA *F CCTGCCTGCCAGACTTTTTGGGGG *F CACCGCCCAACTGTAGGCCGGAGTGCACAATCAGTGCAGAGTGTCCCTCAAATCTCGCTTGCATACGGGAGAGGTGTAT *F AGATCCCTGTCCTGGTTCTTGTGG *F CTATGCCGCCGAGTGTAGTGTGGTAAACCATACGCCAATTTGCGTCTGTCCCGCTGGCTTTACGGGTGATCCGTTCTCC *F AGTTGTCGTCCAGCGCCGCCGCCA *F GAGCCCACACAAA|GCGAATATGTGGATCCTTGCAACCCATCACCTTGTGGACCGAATGCCCAATGCAACGCAGGAATC *F TGTACTTGTCTGGCCGAATTCCATG *F GCGACCCCTACTCAGGTTGCCGTCCCGAGTGCGTTCTCAATTCGGACTGTCCCAGGGACAAGGCTTGCCATAGCAGCAA *F GTGCGTAAACCCTTGTCCAGGAAC *F TTGTGGCGAGAATGCCATTTGTGATGTCATCAATCACATACCCATGTGCAGATGTCCCGAACGCACCGCTGGCAGCGCA *F TTCATCCGCTGCTCTCCTGTGCAG *F ATTACAGTATCTAATCCCTGCCGACCATCACCGTGTGGTCCAAACTCGCAGTGCCGAGAGGTTAATCAGCAAGCAGTGT *F GTTCGTGCCTGCCAAGCTTCATT *F GGAGCTCCGCCATCTTGCCGACCCGAGTGCACCTCGAATTCGGAGTGTGCACCCACTCAAGCTTGCCTGAATCAACGAT *F GTGGAGATCCCTGCCCCGGTACAT *F GTGGAGTGGGTGCTAATTGCGCTGTTGTCAGTCATAGCCCCTTCTGCACATGTCCTGAGAGGTTTACGGGTAACCCATT *F CATTCGTTGCCAGCCCCAGA|TTGA *F ACCCCCCGTTCGTGATGTTGCGCCCGTTGATCCCTGTCGCCCATCTCCCTGTGGACCTTATTCGCAGTGTCGCCCAGTT *F GGTGAGGCCCCGGCCTGTTCCTGT *F GTGGAGACCTATATTGGTCGCCCTCCAAACTGCCGCCCAGAGTGTGTGACCAGCTCGGACTGCTCCAGTCAGCTGGCCT *F GTGTGAACCAGAAGTGTGTCGACC *F CATGTCCAGGACGCTGTGGTCTTAATGCCGAGTGCTTTGTGGTTAGCCACGCCGTGCAATGTATTTGCCAGCAGGGCTT *F CAACGGAGATCCGTTCGTGCAATG *F CAAACCGGAGATCGCTTATGAAAATGAAATCCGCACTCCGTGCAGCCCGAGTCCTTGTGGCCCGAATGCTGTGTGCCGC *F GATCGCAACGGAGTAGGCTCCTGC *F CAGTGCCTGCCGCAATACTTTGGAGATCCTTACGAAGGCTGTCGCCCGGAGTGCATGCTTGACTCGGACTGCCCGTCAA *F ACCGGGCTTGCCAACAGCTGAGAT *F GCCAAGACCCTTGTCCAGGAACCTGTGGACTGAATGCCAACTGTCAAGTGGTGAACCATTTGCCAACCTGTACTTGCCT *F GACCGGATATGTGGGTGACCCATA *F CCGTCAATGTAATCGATTGCCAGAAC|CACCCCAAAACGAGTATGTAAACCCATGTCAGCCGACTCCTTGTGGTCCCAA *F CTCCCAGTGCCGCGTTTCGAATGAA *F CAGGCGGTTTGCTCTTGCCTGCCCCTGTTCGTAGGAACTCCACCATCCTGCCGGCCCGAGTGTACCATATCCTCGGAGT *F GTTCTGCTGATAGGGCCTGTGTGA *F ATCAGAAATGTGTGGATCCTTGTGCAGCAGATACATGCGGAAATAATGCCATATGTAGGGTCCGCAACCACAGCCCTAT *F TTGCAGTTGCATTAGCGGCTATAC *F AGGCGACGCCTTTACTAGATGTTTCCTTATTCCAC|CGCCGATTATTGAGACCAAGGACGAACCTTTAAGGGATCCCTG *F TATACCAACACCCTGTGGTCCCAAC *F TCCGAGTGCCGTAATATCAATGGTGTGCCTGCCTGTTCCTGTCTGGTTAATTTCATTGGCCAGGCACCAAATTGTCGTC *F CCGAGTGCACAATCAACTCCGAGT *F GTCCCAGTCAGCTGGCGTGTATCAACCAAAAGTGTCGCGATCCCTGCCCGGGAGCCTGCGGACAAAACGCTGTTTGCAG *F TGTCATCAATCATACGCCACTGTG *F CGCCTGCATCGATGGTTACATAGGAAATCCATTTACCAACTGCAACCCCAAACCTCCAGAAC|CCCCAGCGCCACCAGT *F TGCGGACGATCCTTGCAATCCGTCG *F CCTTGCGGAGCTAATGCTCAGTGCCGAAATGGCCAATGCTCTTGTATACCTGAATACAAAGGGGATCCATACGTCTCCT *F GTCGTCCAGAGTGTGTGCTTAACA *F CCGATTGCCCAAGGGATCGAGCCTGTGTGCGCAACAAGTGCATCGATCCCTGTTCTGGAACTTGTGGGGTAAATGCCTT *F GTGCGAGGTCAACAACCATATCCC *F AATTTGCCGTTGTCCAGAGCAAATGTCTGGCAATGCCTTTTTCGAGTGTCGACCCGTGCCGCCTGCCAAGATTCAAAAT *F CCCTGCCAGCCGTCGCCGTGTGGT *F CCGAACAGCCAGTGCCGCGTGGTGCAACAAACTGCCGTCTGCTCCTGTCTGGCTAACTACGTAGGCAGTCCGCCGCAAT *F GTCGGCCGGAATGTGTAACTAACT *F CAGACTGTCCTGCCGATCAGGACTGCCAGAATATGAAGTGTCGCGACCCGTGTCCTGGTACATGTGGCTTTAATGCTCT *F ATGCAACGTCGTCAATCACCGTCC *F CTTCTGCAGCTGCCCCACTGGCATGTCCGGAAATCCTTTCGTGAGCTGTCAGCAGCTAA|TTATACGCGATGAAAGACC *F TCAAAATCCGTGCCAGCCTTCTCCT *F TGTGGTCCCAACTCCGAATGTCGTGTATCTGGAGATTCACCGTCTTGTTCATGTCTGCCTGAATTTGTAGGAGCACCGC *F CGAACTGCCGACCCGAGTGTATAT *F CCAACTCTGAGTGCCCCACAAACCAGGCTTGCATCAACCAGAAATGTGTGGACCCCTGTCCAGGACTATGTGGTCAAAA *F TGCCATTTGTCGGGTGTTCAGTCA *F TAGTGCCATGTGCTTGTGTGACGGTGGCTTCACCGGAGATCCGTTTAGTCAGTGTAGTCCAATAAGAGATAGTCCTCCT *F GAAGTTCTGCAGCCCTGCAACCCC *F AGTCCTTGCGGCGTGAATGCCAAGTGCGAGGAACGTGGTGGGGCCGGATCCTGCCAGTGTCTGCCTGATTACTTCGGAA *F ATCCATATGATGGCTGTCGTCCCG *F AGTGCGTTTTGAACTCTGACTGCCCTTCGAATCAGGCATGTGTCAACCAGAAATGCCGTGACCCATGTCCCGGCACTTG *F CGGCCAAAACGCCGAGTGTCAAGT *F GGTCAATCACTTGGCCACCTGTAATTGTTTGGTTGGATACACCGGTGACCCGTACAGCATATGCCGCATCACAGTCAAT *F GAGCCGC|CTGAACGCGTCTATGTG *F AACCCTTGTCAGCCGTCTCCATGCGGTCCAAACTCGCAATGCCGTGAAGTAAACGAGCAAGGAGTTTGTTCCTGCTTGC *F CAGAGTTCATCGGCAGTCCTCCTG *F CCTGTCGTCCGGAATGCACCAGTAGCTCGGAGTGCGCCGCCGACAAGGCTTGTGTGAACCGAAAATGTGTGGACCCTTG *F TCCAAATGTTTGTGGTCAGCAAGC *F CGAGTGTCGCGTGCGTAACCACAATCCCATTTGCACTTGCCTAAGTGGATTCACTGGTGACCCATTCACCCGTTGCTAC *F CGTCAGCCGC|CTCCGCCTCCAGTT *F GTCGAACGTGAACCCCTTGACCCTTGTGTTCCTTCACCCTGCGGTGCAAATTCCCAGTGCCGCGAAATTCACGGCACAC *F CATCCTGCTCTTGCCTTCCGCAAT *F ATTTGGGTACACCACCCAATTGCCGCCCGGAATGTTCCATCAATGCCGAGTGTCCCAGTCATCAGGCCTGTATTAATCA *F AAAATGCAGAGACCCCTGTCCTGG *F CTCTTGTGGTCTAAACACTCAGTGCAGTGTCATCAACCACACGCCAATCTGCAGTTGTCTCGCGGGTTATATAGGTGAC *F CCATTCAGCGTTTGCAACCCCGAG *F CCAATACCCGAGAAAA|TTCGAGACCCACTGCCACCGGAGGATCCTTGCAACCCATCACCATGTGGCTCTAATACACAG *F TGCAACAATGGAGTTTGCTCTTGCC *F TGCCCGAATACCATGGTGACCCATACACGGGATGTCGTCCAGAGTGCGTTCTGCACACGGATTGTGACCGTAGTAGAGC *F TTGTGTTCGTCACAAGTGTGTTGA *F TCCCTGTCCCGGTACTTGTGGAACCAATGCGATATGCGAGGTTCTAAACCATATACCCAACTGCCGTTGTTTAGAAGGA *F ATGCAAGGCAATGCATTTATCCAG *F TGCAGCCCAGTTCCAA|AGCTCGACGTCGTACAGAATCCGTGCCAACCCTCGCCCTGCGGACCAAACTCACAGTGTCGC *F GTTGTTAATCAGCAAGCAATCTGCT *F CCTGCATCACGTCCTTCATTGGAAGTCCGCCATTCTGCCGACCTGAATGCACTACAAACTCGGAGTGCCCCTTGAACCT *F GGCGTGTCGCAACCAGAAGTGCAG *F TGATCCCTGTCCCGGCGTCTGTGGTCGCGGTGCCCAATGTCATGTGACAAACCACAGTCCATTCTGTCGCTGCTTAGAG *F CGCTACACGGGTAATCCGTTTGTG *F AGTTGTCAGCAGATCATCGAACCTCCGGTTCCACCACCACGGCAAACATGCCTGCCATCACCCTGCGGACCGTATTCCC *F AATGTCGTGAAGTTAACGAATCAC *F CTTCTTGCACTTGCCTGCCAGAGTACATTGGAGCTCCACCTAACTGTCGGCCGGAGTGCGTGACTAGCTCGGAGTGTCC *F CACGAATCAGGCGTGCATTCAGCA *F GAAGTGTCGCGATCCTTGCCCAGGACTATGTGGACAATCTGCGGAATGCCGAGTCCTTTCGCATACGCCAAGCTGCGTT *F TGCCCCGAAGGCATGGAAGGTGAT *F CCGTTCACTTTATGTAAGGAAAAGAGGATTCAGGAACTAGACCAGCTAGACCCCTGCAGTCCCAGTCCTTGTGGAATCA *F ATGCCCGTTGTACTTCCCGCCAAG *F ATGCCGGCTCATGCCAGTGCCTGCCAGATTACTTCGGAAATCCTTACGAGGGATGTCGCCCTGAGTGCGTTCTTAATTC *F GGACTGCCCATCGAACAAGGCTTG *F CCAACAGCAAAAGTGTCAGGATCCCTGTCCAGGAACCTGCGGTCAGAATGCTCTCTGCAATGTCCTGAATCATATACCC *F AGTTGTAGCTGTATATCAGGATAT *F TCCGGTGATCCTTACCGCTCGTGTGTCCCAGAAC|CGGTCAAGGAATATGTCAATCCCTGCCAGCCATCACCGTGTGGT *F CCAAATTCTCAGTGCCGTGAAGTGA *F ATGAACAAGCCATATGCTCGTGTCTTCCGGAATATGTAGGAGCTCCGCCTGTGTGTCGCCCTGAGTGTACGATATCTTC *F AGAGTGCCCGGCTGACAAGGCCTG *F CGTGAACCAGAAATGTGTTGACCCTTGTCCAAACACTTGTGGAGATCAAGCGATCTGTCGAGTGGTGAACCACAGTCCG *F ATTTGCTCATGTCGTGCCGGTTAC *F ACGGGCGACGCCTTCTTCCGCTGTTTCCCCAAGCCCC|CGGTACCACCAACACCAGTACAAAAGACACCGGTTGATCCT *F TGTGTTCCCACACCTTGTGGCCCCT *F ACTCCCAGTGCCGATCCCAGGGAGATGCCCCAGCCTGTTCCTGTTTGGTCGGCTACATAGGAGCCCCTCCAAATTGCCG *F ACCCGAATGTCGCATCAACGCTGA *F ATGTCCTAGCAGCCAAGCGTGTATTAATGAGAAATGTAGAGACCCTTGCCCCGGCTCTTGCGGCTATGGTGCCATTTGC *F AATGTGATCAATCACACGCCTAGC *F TGCACCTGTCCACCAGGATACTCGGGTGATCCATTTAGTCAGTGTCAGCCCGTGCCACCTCCGCCTCCAACAC|CCGTT *F AAGCTTGATGATCCATGCAATCCCT *F CACCATGTGGTCCGAACGCTCAGTGCAATAATGGAGTATGTACTTGCATTCCCGAATACCATGGCGATCCCTACAGCGG *F TTGCCGACCCGAGTGCATCACCAG *F TGCCGATTGCTCACGAGAACTAGCCTGTTCGAGAAACAAGTGCTTCGATCCTTGCCCCGGTACCTGTGCCCCCAATGCC *F ATTTGTACTGTCCTTAACCACGTG *F CCCATGTGCACCTGTCCGGAGGGATATAATGGCAATGCATTCGTGCAGTGTAAACCCACACCACGTAAATATTTCCCCT *F CAAGGCCGTGTTCAAAGCCCAACG *F ATACCCAACTCAACGATTTCCTTCCAGCCCCTGCCCTTGTCCAGCCTTGCCAACCATCTCCGTGTGGACCCAACTCTCA *F GTGCCGCGAAGTCAACCAGCAAGC *F TGTTTGCTCCTGTGTGCCAGGATACATCGGAACCCCGCCACTCTGTCGACCGGAGTGCACGTCAAACTCAGAGTGTTTG *F TCACACCTAGCATGTGTTAACCAA *F AAGTGCAATGATCCCTGTCCCGGCTCATGTGGCCGTAATGCCCAGTGCAGTGTGGTTAACCACAATCCCTTCTGTACGT *F GCTTGCCAAGGTTCACTGGCAATC *F CTTTCGTGGGTTGTCAGCAGATTATTGAGCCACCACGTCAAGATATTGTGCCACAGGATCCGTGCCGACCGTCACCTTG *F TGGTCCGAACTCTGAATGTCGCGC *F CGCTGGTGAGACAGCAACATGTACATGCCTTGGTGATTTTGTCGGCTCCCCGCCATATTGCAAACCCGAATGCGTGGCC *F AATTCAGAGTGTCCCTCGAACCTG *F GCGTGTATCAATCAAAAGTGTCGCGATCCCTGTCCTGGATTGTGCGGTTCAAGTGCTACTTGCCGCGTCGTCTCTCACA *F CAGCAATGTGCATTTGTGATGCTG *F GTTTGACCGGAGATCCATTTACACAATGTCAACCCATTGTACAGGATGTTGAGATCATTAATCCTTGCCAGCCGTCACC *F TTGCGGAGCCAATGCCGAGTGCAT *F ACAACGGAATGGAGCCGGAGCCTGCCAGTGTCTCACAGACTATTTCGGAAATCCCTACGAAGGCTGTCGACCGGAGTGT *F GTTTTGAACTCGGACTGCCCATCG *F AACCGAGCTTGTCAGCAGCAGAAGTGCCGTGATCCTTGTCCTGGAAGCTGTGGACAGAATGCCGAGTGTAATGTGGTGA *F ACCATACACCCATGTGTAACTGCT *F TCGCTGGATTCATCGGCGATCCGTATCGCTATTGTAGCCAACCGCCAGAAC|CTATTGTCCATGAGTATGTGAACCCCT *F GTCAACCGTCCCCCTGTGGCCCGAA *F CTCAAATTGTCGCGAGGTTAATGAACAGGCAGTATGCTCCTGCCGTTCCGAATTTGAGGGAGCCCCACCCAACTGCCGG *F CCACAATGCACCTCGAGCTCTGAG *F TGCGCCTCCAACAGGGCGTGCATCAATCAAAAATGCGTTGACCCTTGTCCCGGTGTTTGTGGCCAGCAGGCAATCTGCG *F AGGTGCGCAACCACAGTCCAATTT *F GTAGGTGTCCCACCGCCATGATAGGCGATCCCTTCGTGCGCTGCATACCCCGACCAACAA|TTGCACCGCCACCCTTGA *F GGGATGTAGCTCCCTATCGGGATCC *F CTGCCTCCCCTCACCATGTGGTCTGTATGCGTCGTGCAGGAATCAACAGAACCAAGCGGTTTGCTCTTGCTTGCCCAAC *F TATTTTGGTACTCCGCCCCATTGT *F CGTCCCGAGTGCTCCATTAATGCAGAGTGCCCAAGCCACTTGGCCTGCATTGGTGAACGCTGTCGAGATCCCTGTCCTG *F GTGCTTGTGGTCAACAGACCGAGT *F GCCGTGTGATCAGTCACGTTCCCAGTTGCGTTTGCCTACGCGGCTATGTGGGCGATGCATTCCTGGCCTGCCACCCGGC *F ACCAC|CCCCACCATCCCGTGAAGA *F GCCCCGCGATCCATGCAACCCCAGTCCATGCGGCAGCAATGCTATCTGTTCTAATCAGGGCGAATGTAAATGTGTGGCC *F GACTATCAGGGAGATCCCTATGTG *F GCGTGCAGACCCGAGTGTGTTCTTAGTTCAGAGTGTCCCCGAAATCTGGCCTGTATACAACAGAAGTGCACTGATCCTT *F GTCCCGGAACGTGTGGTACCAATG *F CTATCTGCGATGTGGTCAACCACATTGCCATGTGCCATTGTCCGGATCGAATGACCGGAAATGCTTTTGTCCAATGTAC *F TCCAGTGCAGC|TTGATGTATACCG *F TAACCCTTGCAATCCCTCGCCATGCGGCTCCTATGCTGAATGTCGGGAGCAGAACGGTCAAGCGGTGTGCAGTTGTCTT *F CCCAACTACTTTGGGGTACCACCC *F TCGTGTCGACCCGAGTGTAGCACCAACTACGATTGTTCCCCAAGCTTGGCGTGTCAGAATCAGCGCTGCGTCGATCCTT *F GTCCTGGAGCTTGTGGCGCCTACG *F CCGAGTGCCGAACAGTCAACCACAGTCCTTTCTGTAGTTGCAGACCCGGCTATACGGGAAATCCCATTGTCCAATGTCA *F TATGATTA|TTGAGCCCCAGCGGGA *F TATCACACCCAAAGATCCTTGCCAACCCTCACCCTGTGGTCCAAACAGCGAATGTCGACGCGTAGGAGAAACACCCTCC *F TGCTCCTGCCTGTCCAATTTCTTT *F GGCACTCCGCCCAACTGTCGACCTGAATGTGTCTCCAATTCCGAGTGCAGTCAGGTGCATGTCTGCTCAAATAATCGCT *F GCAAGGATCCGTGCCCGGGTCTCT *F GCGGAACTGATGCCGTCTGCCGCGTGATAAGTCACAGCGCTATGTGCTACTGCCAGCCGGGTTACAGCGGCGATCCCTT *F CGTCCGTTGTGCACCACACATTCA *F AAGGGAATCGATTGAGATAGTGCAGCCCTGCAACCCCAATCCCTGCGGCGCCTTTGCCGAGTGTCGTCAGCAGAATGGA *F GTCGGATCGTGTCAGTGTCTACCA *F GAGTATTTCGGCAATCCTTATGAAGGATGTCGTCCTGAATGTGTACTTGACTCTGATTGCCCCTCTCAGCTGGCCTGTG *F TCAATCAAAAATGCCGTGATCCCT *F GTCCCGGAAGCTGCGGCCAAAATGCCGAATGCTTTGTGCGCAATCACCTGCCCACTTGTAACTGCCTCAGTGGCTATGT *F GGGTGACCCATATCGGTATTGTAG *F CATCGAACCGAAAC|CCATTCGGGAATATGTCAACCCCTGTCAACCCTCTCCTTGTGGTCCTAACTCTCAGTGCCGCGA *F ACAGAATGGTGTGGCCACCTGCTCT *F TGCTTGCCAGAATTCGTTGGCACTCCGCCGGGTTGTCGACCGGAATGTACAGTATCCTCTGAGTGCAATCTTGACAAGG *F CCTGCGTCCGTCACAAGTGCCTGG *F ATCCTTGTCCTGGTGCTTGCGGCAGCTCCGCCAATTGCCAGGTGGTGAACCACGCTCCACTCTGCTCTTGTCAAGCGGG *F TTACACAGGAGACCCATTTACTCG *F ATGTTACCCAATTCCTT|CTCCACCTACGCATATTGTCCATGATTACGCACGCCATCCATGCCAACCATCTCCCTGCGG *F AGCTAATGCTCAATGTAGACAATCC *F CAAGGTCAAGCCATCTGCTCCTGTATACCCAACTACTTTGGTGTTCCACCCAATTGTCGACCCGAGTGCACTCAAAGCT *F CAGAGTGCCTGTCCAGTCTGGCCT *F GCATCAATCAACGATGCGCGGATCCCTGTCCTGGCTCCTGTGCCTACAACGCCATATGCCATGTCCGTAATCACGTGCC *F CAGTTGTCAGTGCCCAGTGGGCTA *F TGTGGGTGATCCGTTCACCAACTGTCACCCTGAACCTCAGCCACCAC|CCAAACCCGTTGCTCTCGATGATCCCTGCAA *F TCCTTCCCCATGTGGGGCCAATGCA *F GTGTGCCAAAATGGTCAATGTTCGTGTATACCCGAGTACCAAGGAGATCCTTATACCGGCTGTCGGCCGGAATGTGTGT *F TAAATGCGGATTGTCCGCGAAATC *F GTGCTTGTGTTAGACATAAGTGTGTGGATCCGTGTCCGGGAACGTGTGCACCGAATGCCATCTGCGATGTGATTAACCA *F CATTGCCATGTGCCGTTGCCCAGA *F GCGTATGACAGGCAATGCCTTCATCCAATGTGAAACACCGCCAGTGTCGCTGGCACCGCCAGATCCGTGTTACCCATCG *F CCCTGCGGTCCGAACAGTCGCTGT *F CGTGTGTTCAACAACAACGCTGTGTGTTCCTGTATTGAGGATTTCATTGGAACCCCACCAAATTGCCGACCGGAGTGTA *F CGCATAACTCAGATTGCCTGCCTA *F GACTGGCGTGCCAGAGGCAGCATTGTATAGACCCGTGCCCAGGAACTTGTGGCTTTAATGCCCTTTGCCACGTTGTGAA *F CCATGCACCAATTTGCAGTTGTCC *F ACCGAAGCATAATGGAAACCCCTTCTTGGGATGCTTCCCGGAACCCGTGCGACGCGATGAGGTGATTCCAAAGAATCCG *F TGCCAGCCCTCGCCATGTGGTCCA *F TATGCTAAGTGCACATCCGTGGGCGATCAGGCGCAGTGCAGTTGTCTGCCTGAGTACATTGGAACTCCGCCCAACTGTC *F GCCCAGAGTGTATCACCAATTCGG *F AATGCTCCTTCGACAAAGCCTGTTTAAACCAAAGATGTCGCGATCCCTGCTCGGGAACTTGTGGTTCCAACGCCAATTG *F CCATGTAATTAGTCATACTGCCAT *F GTGCTACTGTCTACCAGGTTTTACTGGTGACCCATTCACTTCCTGTGTCCAAGTACCAGTGATTCAGCAGGCTGAGATC *F GTTCAACCATGCTCTCCTAATCCT *F TGTGGAGCCAATGCTGTATGTCGCCAGGAGGGCCATGTTGGTTCTTGCCAATGTCTTCCCGAATACTACGGAAACCCAT *F ACGAGACATGTCGGCCGGAGTGTG *F TAACGAATAACGACTGTCCTTCCAATAAGGCTTGTCAACAGCAGAAGTGTCGTGATCCTTGTCCAGGAGTCTGTGCCCT *F CAATGCTTTGTGCCGCGTGATCAA *F TCACTTACCAACTTGTCATTGTCAGAATGGCTTTGTTGGTGACCCATATCGCTATTGCCAAATTCCCGAGAAAC|CTGT *F CCTTAAAGAATATATCAACCCCTGC *F CAGCCCTCCCCGTGTGGCCCCAACTCGCAGTGCCGTGAAAACAACGAACAGGCCATCTGCTCATGTTTGCCCGAGTACG *F TCGGTGCTCCACCTAATTGTCGAC *F CTGAGTGCGTGACCAGTGCAGAGTGTCCTCATGACAAGGCGTGCATTAGGCAAAAGTGTAATGATCCCTGCCCCGGTGT *F ATGTGGCTCAAATGCCGATTGCCG *F TGTTATCCAGCATGCTCCTATTTGTAGTTGTCGTGCAGGATTCACAGGCGATGCCTTTTCACGCTGCTTGCCCCTGCCA *F C|CCTCGAGACCACCGCAGTTGGAT *F GTATACCGCAATCCTTGTGTACCTTCGCCTTGTGGCCAGTATGCAGAGTGTCGGGATAACCAGGGCACTGCCACGTGCA *F GTTGTCTGCCCTCATATTTTGGCA *F CCCCGCCCAACTGTCGCCCCGAGTGTACGATAAATCCCGACTGCCCCTCTCACTTGTCCTGCCAACAACAGCGTTGTCG *F CGATCCTTGTCCTGGAGCCTGTGG *F ATTCAATGCGCTGTGCACAGTTATTAATCACAATCCGACGTGTCAGTGTGCTCCAGGATTTATTGGCAATGCCTTTACT *F TCCTGCCATGTTCCACCTCCAATT *F GTTCGGGACCCCCCGCAAATAAGTGATCCATGTGATCTGATCACTTGTGGCCCCAATGCTGTTTGCAATCAGGGACAAT *F GTAATTGTCTGCCTGAATTTGTGG *F GCAACCCATTAGTTGGATGCCGCCCGGAATGTGTGTTGAGCACAGAATGCGACTGGAGCAAGGCGTGCGTGAGAAACAA *F GTGCATCGATCCATGTCCAGGAAC *F TTGCGGATCCAATGCCATATGTGAGGTGCACAGGCATATAGCCATGTGTCATTGTCCACCGGAAATGACTGGAAATGCA *F TTTAGCCAATGCAGACCCTTGCCA *F CCAGCTCCGGTACGCGATGTTATAGACCCTTGTCAGCCTTCGCCATGCGGACCAAATGCCCAGTGTCGCAATATCAACG *F GACAAGCAGTGTGCTCATGTCTTC *F GGGACTTCATCGGAGTTCCACCTTCATGTCGGCCAGAGTGCGTTAGTAATGCCGAGTGCCCACTTCATCTGGCTTGCCT *F CCAACGACATTGCCGAGATCCTTG *F CCCCGGAGTCTGTGGCCTGAATGCCGAATGCCGGGTCATAAACCACAGCCCGAACTGCCATTGCATTGGTTCCTTCACG *F GGAAATCCATTCGCGGCTTGTCAC *F CGGCCACCGCCGCCACCAATTAAGCATGAGCCCATTGATCCTTGTCAGCCATCGCCATGTGGAGCTAATGCGGAATGCC *F GTGTCCAGGGAAGCAATGCTCAAT *F GCAGTTGTCTAAGTGGCTTCATCGGAACACCACCCAACTGCCGACCCGAATGCGTATCCAACTCGGATTGTCCCATGAA *F TCTGGCTTGCCTCAATCAGAAGTG *F CCGAGATCCCTGTCCAGGTGTCTGTGGCTCCAATGCGGAATGTTATGTGATAAACCATACACCCATGTGCACATGCCTT *F GCTGGACAAACCGGCAATCCTTTC *F GTAAGCTGTCAAGTGGTGCGAGATGTTCCCGAGCCACAAACTCCTTGTGTGCCCAGTCCATGCGGAGCCAATGCACTCT *F GTTCTGAGGGAAATGGAGCCGGAG *F CCTGTAAATGCCTTCCGGAATTCTATGGCAATCCCTATGAAGGCTGCCGACCGGAGTGCGTTCTCAATTCGGACTGCCC *F CAGCCACTTGGCTTGTCTCAACCA *F GCATTGCCGCGATCCCTGTCCCGGAACATGTGGTATCAACGCCGAATGTCAAGTGCGCGACCACTTGCCCCAATGCAAC *F TGCCATGTGGGATATCAGGGAAAT *F CCCTACGTTTACTGTAGTGTTCTGCGTGATC|CCCTGCCTGAACCAGTTCCCTCCCGCCCTTGTCAGCCGTCACCTTGT *F GGTCCAAACTCTCAGTGTCGGGAAT *F CGAACAACCAAGCAATTTGCAAGTGTCTGCCCAACTTTATTGGATCCCCACCTGCGTGTAGACCCGAGTGCACCATCTC *F CAGTGAATGTGATTTGACCCTGGC *F TTGTGTTCAACAACACTGTGTCGATCCCTGTCCCGGTGTCTGCGGCAATAGTGCCCAGTGTCGGGTCATAAATCACAGT *F CCTCACTGTAGTTGCTTGCCTGGT *F TTCACTGGTGATGCCATTAGTGGCTGCCAACGGATTC|CACCCGCAATTACCCACGATGCCCCCAATGAGACCCCTCGC *F GATCCCTGTGTCCCCTCCCCCTGCG *F GAGCCTTTGGACAGTGCCGTGCTCAAGGAAACCAGGCTATATGCTCATGCCTTCCTGGCTACTACGGAGCACCTCCAAA *F TTGCAGGCCGGAGTGCGCAATAAA *F CCCGGACTGCGCCTCCCATTTGGCGTGTATCAGTGAAAAGTGTCGCGATCCATGTCCCGGTTCTTGTGGCCTGCAGGCC *F CAATGCAGTGTCATAAACCACACA *F CCGATCTGTAGTTGTCCATCAGGTTATGAGGGCAATCCCTTCGTCAGGTGTCAACGAACCCCTCCAACGCCGACACCGC *F CCCTCCACGATGCCTGTAATCCCT *F CACCTTGTGGCTCCAACGCTATCTGCAGCCCCGGAGGACAGTGCTCATGTCTGCCCGACTTTGATGGCAACCCCTACGT *F GGGCTGCCGCCCTGAGTGCGTCCT *F CAACACGGACTGTGCCAGAGACAAAGCCTGCCAGCGCAGCAAGTGCACCGATCCTTGTCCCGGAGCATGTGGTATTGGA *F GCAGTTTGCGAAGTTCGCAATCAC *F ATTCCCACGTGTAATTGCCCACCCGGAACTTCGGGCAATGCTTTCGTTCAATGTACCCTTGTACAAT|CTTCACCCGTG *F GTGCCCTTAAACCCTTGTCAACCAT *F CCCCGTGCGGAAACAATGCCCAATGCCGAGAGGTTAACGATCAGGCAGTTTGCTCTTGTCTACCAGGCTTCTTTGGTGT *F TCCACCAAAGTGTCGTCCTGAGTG *F CACCATTAACTCAGACTGTGCCCCACATCTGGCATGTTTGAACCAACAGTGCCGGGATCCTTGCCCTGGAGCATGCGGC *F CAGTTCGCTCAATGCCAGGTCATC *F CGACATGTGCCGCACTGTAGTTGTCCCGCAGGCTTCTCAGGCAATGCTTTCTTCCTGTGCCAGCGCTTACCGCCACCAC *F CACCAGTCCAACGGGAACCGATCA *F ACCCATGCTACCCATCGCCATGCGGACCCAATGCAGAATGCACAAATCAGAATGAACAGGCCATATGCAAGTGCCTGAA *F GGACTACATAGGAACACCACCCAA *F CTGTCGACCGGAGTGCATAACGTCATCAGAGTGTCCGATTCAGCTGGCTTGTATTGGTCAAAAATGCAAGGATCCTTGT *F TCTGGACTTTGTGGAATTGCTGCC *F ACCTGTCAAGTTGTCAGCCACGTGCCTTCGTGTATTTGCATTGCGGACTATATTGGAGACCCTTACACTGGATGCTATG *F CGCGGCCGCCCATTCAGCGAGAGC *F AAATCAATCCATGCTACCAGAACCCGTGCGGAAGCAATGCGGTCTGCAGAGAGCGTGGAGAGGCTGCTTCGTGCCAGTG *F TCTACCGGAATATTACGGTAACCC *F GTATGAAGGATGTCGACCCGAATGTGTGCTTAACTCGGACTGCTCCAGCCATTTGGCTTGCCTCAATCAACACTGTCGA *F GATCCCTGTCCAGGAAGTTGTGCC *F CCTAATGCCCAATGTCAAGTAGTCAATCACGTGCCCAGCTGTAGTTGTTATCCTGGATATAGTGGCGATCCCTATCGCC *F ATTGCCATGTGGCTCAGGCAGAAC| *F CCGTCCAAGTTGTGCACTTTAATCCCTGTCAACCCTCGCCATGTGGTCCCAACTCACAGTGCACAGAGTCTCAGGGTCA *F AGCAGTGTGTCGCTGTCTACCCGA *F CTACTATGGTTCCCCACCTGCTTGTCGACCCGAGTGCACCACTAATCCCGAATGTCCCAATGATAAAGCCTGCGTGAGC *F AGAAGATGCACCGATCCTTGCGCG *F GGTGCTTGTGGTCAGAACGCTATTTGTCGAGCGCATCAGCACAGAGCTTATTGCTCATGTCATCCTGGTTATACAGGTG *F ATGCCTTCATGCGTTGCCAGTCCT *F TGCCTTCGCCCCAACCGATTAGGGACTCACCAGTAATCTATAGAGATCCCTGTGTACCCTCGCCGTGTGGACAGTTTGC *F TCAGTGTCGCGTGGAGTACGAACA *F AGCGGTATGCTCTTGTCTTACCTCCTACTACGGTACTCCACCATACTGCCGACCCGAGTGCACTCAGAATTCAGATTGC *F CCCAGCCATCGGGCATGCGTCAAT *F CAAAGGTGCGTGGATCCCTGCCCCGGGGCTTGTGGTCTCAACGCTCGCTGCGATGTGCTCAATCACGTGCCTAGCTGTT *F CATGTCCCGAGGGTTATTTAGGAG *F ATCCCTTCTACCGCTGTTACCCTGCTCCCGCGCCTCCTCCAACACCAGTTACCGTCGTAGCCGATGACCCTTGTCAGCC *F CTCACCATGCGGTCCCAATGCACA *F GTGTTCGAACGGCGTCTGTAGTTGTCTGCCCCTGTACCAGGGTGATCCCTATGTGGGCTGTCGCCCAGAGTGCGTACTA *F AGTACTGAGTGTCCGTGGGACAAG *F GCCTGCATCCGTAATCGTTGCTTGGACCCGTGTCCCGGAACGTGCGGATCAGGAGCCACATGTCAGGTACACAATCATG *F TAGCCATGTGTCAGTGTCCGGTTG *F GCTATCAGGGCAATCCTTTTGTCCTGTGCCAGCAGACACCTCTTCAAGCACCCGTGGAGCTCCATCCCTGCCAGCCATC *F ACCTTGTGGACACCATGGAGAGTG *F TCGGGAAGTTGGTTCGCAGGCCATTTGCACTTGCCGTCTCGGTTATTATGGCAGCCCACCGGCTTGTCGACCCGAATGT *F GTCAGCGATCCGGAGTGTCCACCC *F AGTTTGGCCTGTGTAAATCAGAAATGCCGCGATCCCTGCCCCGGTGCTTGTGGTCACCTAGCTCAATGTCATGTAATTA *F ACCACAGTCCACAGTGTGTGTGCC *F CTGCTGGATACACTGGTAGTCCTTACTCCGAGTGCCACTTAATTAGGGCAGATTCCTCACCAATTCAGCGTCAACCAAT *F TGATCCCTGTTTGCCATCTCCCTG *F TGGTCCTCATGCTCAATGCAGCAATGAGGGAGGCAACGCAGTTTGCCGATGCCTAACGGAATACCTGGGAGTACCGCCA *F TACTGTCGACCCGAATGCATTGCC *F AATAGCGAGTGTCCCAGTGACAGGGCGTGTATTAATCGGAAGTGCCAGGATCCATGTCCCGGCCTGTGCGGGTACAATG *F CCATTTGTCGAACCTACAATCACC *F AACCAAACTGCGTTTGTGCTCCTGGTTTGGTGGGTAATCCCTTTAACTCCTGCCTGCCCCCAACGCGTCCTGAGATTCC *F AGCAACACCTCCCACAACTGCCAT *F ACAAGTGCTGCAGTATGAGGAACCATTCATCAATGGTTGTGAACCAAATCCTTGTGGGGCAAACGCGCAATGCAATCAG *F CGAAGGGGAGTGGTCTCCTGCGTC *F TGTCTGCCAGACTATTTTGGCAACCCCTACGAAGCGTGCCGACCCGAGTGTATTCTGAACTCTGATTGTCCCTTAAGTA *F GAGCTTGTGTACAACAGAAATGTC *F GCGATCCATGTCCCGGAACTTGTGGCCTGAATGCCGAGTGTCATGTCATGGATCATTTGCCACAGTGCAGATGTTTCAG *F TGGGTATACTGGAAATCCCTTGGC *F GTACTGCTCGCCTGTTCCAATAATCCAAGAAT|CTCCCCTGACCCCCTGTGATCCCTCACCTTGTGGACCCAATGCCCA *F GTGTCATCCGTCCCTCAATGAGGCC *F GTGTGCTCCTGTTTACCCGAATTCTATGGAACCCCACCCAATTGTCGACCCGAGTGTACTCTAAACTCGGAGTGTGCTT *F ATGATAAGGCTTGCGTCCATCACA *F AGTGCGTTGATCCTTGTCCTGGAATTTGTGGTATTAATGCGGATTGTCGCGTGCATTACCATAGTCCCATATGTTATTG *F CATTTCTTCGCATACGGGTGATCC *F ATTCACACGATGCTACGAAACCCCAAAAC|CTGTGCGACCGCAAATATACGACACGCCCTCTCCCCCATATCCGGTCGC *F TATACCCGATCTAGTGTACGTGCAA *F CAACAACAACCGGGAATAGTAAATATTCCCTCTGCCCCTCAACCGATATATCCTACGCCCCAGTCACCACAATACAATG *F TAAACTATCCATCTCCGCAACCGG *F CGAATCCCCAAAAACCGGGAGTTGTGAATATACCATCTGTGCCACAGCCAGTTTATCCATCACCGCAACCACCTGTTTA *F CGATGTCAACTACCCAACAACGCC *F AGTTTCCCAACATCCCGGGGTCGTGAACATTCCTTCGGCACCTCGACTGGTGCCTCCGACATCTCAGAGACCTGTGTTT *F ATAACATCGCCAGGGAACCTATCA *F CCCACACCTCAACCTGGAGTTATTAATATTCCTTCAGTTTCACAACCTGGATATCCAACGCCGCAAAGCCCCATTTATG *F ATGCAAACTATCCTACTACACAAA *F GTCCTATTCCCCAACAACCTGGCGTAGTTAATATTCCATCGGTGCCAAGTCCATCTTATCCGGCTCCAAATCCGCCGGT *F GAACTATCCAACGCAACCATCACC *F TCAGATACCAGTTCAACCGGGAGTTATCAATATACCGTCTGCGCCGCTTCCAACAACGCCACCACAACATCCTCCCGTT *F TTTATTCCGTCCCCTGAGAGCCCG *F TCACCAGCTCCAAAGCCTGGAGTAATTAATATTCCATCCGTAACGCACCCAGAATACCCAACATCGCAGGTTCCCGTTT *F ATGACGTCAATTATTCAACAACAC *F CGTCTCCTATTCCTCAAAAGCCTGGAGTAGTAAACATTCCTTCAGCACCGCAGCCAGTGCACCCTGCACCCAATCCACC *F AGTCCACGAATTTAATTATCCAAC *F GCCGCCTGCAGTTCCTCAACAACCAGGCGTTCTTAACATACCATCGTACCCAACGCCAGTGGCACCGACTCCTCAATCT *F CCTATATATATACCATCCCAGGAG *F CAACCGAAACCAACCACAAGGCCATCAGTTATAAATGTGCCTTCAGTTCCACAACCTGCATATCCTACACCACAGGCAC *F CTGTTTACGACGTTAATTATCCAA *F CATCTCCATCTGTAATACCACACCAACCTGGAGTAGTTAACATTCCATCAGTGCCACTTCCAGCACCTCCAGTTAAACA *F GCGTCCTGTGTTTGTACCATCACC *F AGTACATCCTACTCCCGCTCCTCAACCAGGTGTTGTTAATATACCTTCAGTGGCACAGCCCGTGCATCCCACGTATCAA *F CCACCGGTTGTGGAACGGCCTGCT *F ATATATGACGTGTACTACCCGCCGCCGCCATCTAGACCGGGTGTGATCAATATTCCTTCGCCACCAAGGCCTGTATACC *F CAGTGCCACAGCAACCGATCTACG *F TTCCGGCACCAGTTCTACATATACCAGCTCCAAGACCAGTCATTCATAACATTCCTTCAGTGCCTCAACCAACATATCC *F ACATCGTAATCCTCCGATTCAGGA *F TGTTACTTACCCAGCTCCACAACCAAGTCCTCCTGTACCAGGAATAGTAAATATTCCATCACTGCCTCAACCCGTGTCA *F ACACCCACTTCTGGAGTGATAAAT *F ATTCCCTCGCAAGCATCTCCACCGATCTCAGTACCAACTCCAGGAATTGTAAACATTCCATCAATACCGCAACCAACGC *F CACAGCGTCCTTCACCAGGAATTA *F TTAATGTTCCTTCAGTGCCTCAACCTATACCAACTGCTCCTTCACCTGGAATTATTAATATTCCTTCTGTACCTCAACC *F GTTGCCATCGCCAACTCCTGGTGT *F GATCAATATTCCGCAACAACCCACGCCACCACCTTTGGTTCAGCAGCCCGGAATTATAAACATTCCGTCGGTACAACAA *F CCATCAACTCCAACCACTCAGCAT *F CCTATACAGGATGTTCAATATGAAACCCAGAGACCACAGCCTACCCCTGGAGTTATCAACATTCCATCTGTGTCACAAC *F CTACGTATCCAACTCAAAAGCCAT *F CTTACCAGGATACCAGTTACCCAACAGTACAGCCTAAGCCTCCGGTATCTGGAATTATAAATATTCCCTCGGTGCCCCA *F GCCTGTGCCATCATTGACTCCTGG *F GGTGATTAATTTGCCTTCAGAGCCATCCTACTCGGCACCAATTCCAAAGCCTGGAATCATCAACGTTCCCTCGATACCG *F GAACCTATACCATCAATACCGCAA *F AATCCCGTACAAGAGGTTTATCACGATACTCAGAAGCCACAAGCAATTCCCGGTGTGGTAAATGTTCCATCTGCTCCAC *F AGCCAACTCCAGGTCGCCCCTATT *F ATGATGTGGCCAAACCAGATTTCGAGTTCAACCCGTGCTATCCATCGCCTTGTGGTCCCTACTCTCATTGTCACAATCG *F ATTTGGAGTAGCTGCCTGTGTTTG *F CCTACCCAATTACAGAGGAACTCCACCGAATTGCCGACCAGAATGCGTTATTAATTCGGACTGTCCATCATCTTTGGCC *F TGCATTAACGAAAAGTGCCGAGAT *F CCTTGTCCAGGATCTTGTGCTTACAATGCCGTGTGCCGTGTGCATGAGCATGTGCCTAATTGTTACTGCCAGACTGGCT *F ACACCGGAAATCCATTTATTTCCT *F GTCAACGTACACCTATAGCTCCTGTTCAACGTGAGCCGATTGAGGCGAAGGATCCATGTTATCCATCGATTTGCGGACC *F AAACGCTGTGTGCAATAATGGAAA *F GTGCAGCTGCATACCTGAATATCGAGGAGATCCCTATGTTGGGTGTAGGCCAGAATGTGTTCTCAATACAGACTGTGCT *F AGGGATAAGGCTTGCATTCAACAA *F AAATGCAAGAATCCGTGTCCAGGCACTTGTGGACTACAGGCCTTGTGTCATGTGTACAACCATGTGGCCACCTGCTCTT *F GTCCGGAAGGAATGCAGGGCGATG *F CCTTTGTGAGATGTGATCCAAAACCCAAGCCTCAACCGCCTGCACCAGCACCACCCACAACATTGCCGGCCATCGTACC *F GCAACGTGCTCCGATTAATCCCTG *F CCAGCCGACTCCCTGTGGACCCAATTCCCAGTGCAGAGCTTATCATGAGCAGGCGATTTGCTACTGCCTGCCGAATTTC *F ATTGGCACGCCACCAGGCTGTCGT *F CCGGAGTGTACCTCCAACTCGGACTGTCCCCTCGACAAATACTGTCTGAATCTCAGGTGTCGCGATCCTTGTCCCGGAG *F CATGTGGCATTCGTGCCATTTGTC *F ATGTCCAGAACCACGGTCCCTTGTGTGTGTGCCCACCCCATTTGACGGGTAATCCCCTACTGGCCTGTCAACCCATAG| *F TTATTCCCCCTGTAGAGCGTGACGA *F AGTGAATCCCTGCCAGCCCTCGCCTTGTGGCCCGAACTCCGAGTGCCAAGCCACGTCCGGCGGAGCTCGCTGCTCCTGC *F CTACCCCAGTATCATGGCACTCCG *F CCCTTCTGTCGGCCGGAGTGCGTGAACAGCGCTGACTGTCCGGCGGACAAGGCGTGCCGTAACTACAAGTGCATTGATC *F CCTGCCCCGGCAGCTGTGGTTTCT *F CAGCTCTCTGTCGTGTGGTGGCCCACAGTCCTGTGTGCTATTGTCCCGAGGGCTATGTGGGCAATGCCTACACCCTTTG *F CTCTCGACCAGAGCCGAGTCCACC *F TGCCGTGGTGATCCTGCCTTGCAATCCCAGTCCCTGTGGCGTAAACGCATTCTGTCAGCCGCACAACGACTTGAGCGTT *F TGCCAATGCCTGCCGGGTTACTAT *F GGCAATCCCTCTGAGATTTGCCGACCCGAATGCACCGTCAACTCGGACTGTCCAAGCCACAGGGCTTGTATGAGCGAAA *F AGTGTCGCGATCCTTGTCCCGGAG *F TTTGTGGTCTTAATGCCCTCTGTCAGGTCATTAACCACAGCCCAGTTTGCGAATGCCACACGGGGCATGTGGGCAATCC *F CTATCACAGCTGTCGCATACCACA *F GCGTGAAC|CCCCTGCACCGGAATACGTGAACCCCTGTCAGCCGTCGCCTTGCGGTGCCAATTCTCAGTGTCGCGAGTC *F CCAGGGACAGGCTATCTGCTCCTGT *F CTGCCGGAGTTTGTGGGCACACCGCCCTCGTGTCGTCCGGAATGTGTGATCAGCGCCGAGTGTCCAGCGGATAGGGCGT *F GCATTAACCAGAAGTGCCAGGACC *F CTTGTCCAGGAGCATGTGGCTTGAATGCCCAGTGCCATGTGCGCAATCACAGTCCGCTGTGCTCGTGTCAGCCCGGATT *F CACCGGAGATGCTCTGACCCGCTG *F TCTACCAGTACCGCCTCCACAACCGCCGAAATCGAATGATATTCGCGATCCTTGTGTGCCATCTCCTTGTGGACCTTAC *F TCCCAATGCCGGGTGGTTAATGGC *F GGAGCTAGTTGCAGCTGCCTTCCCAATTATGTGGGAGCGGCGCCCAACTGCCGTCCGGAATGTACCATCAATGCGGAGT *F GTCCCAGTAATCTGGCCTGCATCA *F ACGAGAAGTGTCGCGATCCTTGTCCTGGAGCCTGTGGCTTTGCTGCCCAGTGCAGTGTCATCAATCATACGCCCAGTTG *F TTCGTGTCCCGCTGGTTACACGGG *F AGATCCGTTTACCAGCTGCCGAGTTCTGCCACCACCACCGCCTCCGAAGA|CTCCTTCCGATCCCTGCCAGCCTTCGCC *F CTGCGGAGCCAATGCTCTGTGCAAC *F AATGGCCAGTGCTCTTGCCTGCCAGAGTATCATGGTGATCCTTACACTGGATGCCGTCCCGAATGCGTTTTGAATTCTG *F ATTGTCCCAGGAATAGAGCGTGTG *F TCAACCAGAAGTGCGTTGATCCTTGCCCAGGTCACTGTGGTCTCAATGCTCTCTGCGATGCCGTTAACCACATTGCCAT *F GTGCCACTGCCCCGAGCGGATGAC *F GGGCAATGCGTTCGTTTCCTGCCAGCCAATTCGCGATGATCCACCACCACCAACGACACCCAATCCATGCCAGCCCTCG *F CCGTGCGGTGCAAATGCCCAGTGC *F CTAGAGAGGAATGGCAACGCCATCTGTTCTTGCCTAGCCGGATACTTTGGTCAGCCGCCCAATTGCCGTTTGGAGTGCT *F ACTCCAGCTCTGATTGCTCCCAAG *F TGCACAGCTGCATTAACAACAAGTGTGTGGACCCGTGTCCAGGCAAATGTGGTCTAAATGCTGTGTGCCAGGCGATCCA *F GCACAGAGCCCATTGCGAGTGCAT *F CCCGAGATACACAGGAAATGCCTTTGTGCAGTGCAACCCCATTCCAGTTCCCAGAGTACCAGAACCAGTGCGCGATCCT *F TGCCAACCGTCACCCTGTGGTCCC *F AACTCGCAGTGCACGAATGTAAATGGCCAAGCGGAGTGTCGCTGTCTGCAGGAGTTCCAGGGTACCCCGCCAAACTGCC *F GACCAGAGTGTGTAAGCCACGATG *F AGTGTGCCAATACCCTGGCTTGCATGAACCAAAAGTGCCGCGATCCCTGTCCCGGCAGCTGTGGCCAGAGTGCCCAGTG *F TACGGTCAGCCTACACATTCCGAA *F CTGTCAATGCCCTGTGGGCATGACGGGTGATCCATTCCGAATTTGCCTGCCCAAACCACGTG|ACGAACCAAAGCCACC *F ACCAACTCCCAAGAATCCTTGCTAC *F CCTTCGCCCTGCGGAACCAATGCTGTGTGCCGCGTTCAAGGAGAGAACTACGTCTGCGAGTGCAGCCAACTGGAGTACA *F TCGGCAATCCCTACGAGGGTTGTC *F GCCCCGAATGTGTGGGCAACTCCGAGTGCCCGGCCAATCAGGCTTGCATTCGCAGCAAGTGCCAGGATCCTTGTCCAGG *F AGTTTGCGGACTGGAGGCCATTTG *F CACCATGAACAATCACATACCGATCTGCTCTTGCCCGCCTGGCTACACTGGCAACGCGTTTGCCCAGTGTACGCGTCAG *F GTAACACCACCTCCTCCCTCGGAT *F CCTTGCTACCCGTCCCCATGTGGACCCAATTCGATATGCCGAATTCAGAACGAGAAGGCCGTGTGCGAGTGCCTGCCCG *F GATTCTTCGGAAATCCGCTGGCCC *F AGGGATGTCGCCCTGAATGCACCCTGAGTTCGGACTGCGCCAAGGATCGGGCATGCATCAACTCCAAGTGCGTGGATGC *F CTGCGTGGGAGAGTGTGGATTCGG *F AGCTGTTTGCCAAACGATCAACCACAGCCCGGTGTGTAGTTGCCCTGCCAACATGGTGGGCAATCCGTTCGTCCAGTGC *F GAGGAACCACGCCAGGCTGAGCCT *F ATCGATCCCTGCCAGCCTTCACCGTGTCGAAGCAACGGAATATGTCGCGTTTACAATGGAGCTGCCACCTGCAGTTATC *F CGGAATGCGTTATCAACGAGGATT *F GTTCCCGCGACAGGGCGTGTGTTAGCCAAAAGTGTCGAGACCCTTGTCTGAATGCCTGTGGCATCAATGCCATTTGTCG *F GGCCATCAACCACAAGGCTGTTTG *F TAGCTGCCCACCGGAGTTCTACGGATCTCCGTACGCTCAATGTCTGAGGCAGCTACCGGAGCCAGAGCCCAAGCCGGAA *F TGCATCAGTGACGGTGATTGCACC *F AATGACAAGGCCTGCATCAACCAAGTTTGCCGCAATCCATGCGAGCAATCGAACATCTGCGCCCCACAGGCGCGTTGCC *F ATGTCCAGCTCCACCGGCCATTGT *F GCGTCTGCAATGAGGGATATACTGGAAACGCCTTGCAGAACTGCTACCTTCTGGGATGCCGATCGGATGGTGAATGTGC *F CGCCAACGAGGCTTGTGTTAACCA *F GCAGTGCGTAGATCCATGTGGTTTCACACAGTGCGGAACGGGAGCCATTTGTCGGGCTGACTTCAATCACCGAGCTAGG *F TGCCATTGCCTGGACGGATACCGC *F GGCAATCCTCTGGTGCGTTGTGAACGTCCAGAGTGCCGATCCGATGATGAATGTGCCTTCCACCTCGCCTGCCGCAATG *F AACGCTGCGAAGACCCGTGCAACT *F GTGGCATCGGAGCCCAGTGCCGCGTGGAGAACCATCGTGCCCAGTGTCGTTGTCCAGCTGGCTTCAGTGGCAACCCGGC *F AGTAAGATGTGACCTTGTGCCAAC *F ACAACCGGAGGGCTGCACCATGGATGCCGAGTGTCCCAGCAAACTGGCCTGTTTCGGCGGCGAATGCAAGAACCCGTGC *F GATGTCACGCATCCGTGTGGCGCC *F AATGCCATCTGCGAAGTGGTCGACACACTGCCTCTGCGCACCATGATGTGCAGTTGTCTACCTGGCTATGTGGGTGAAG *F CCGACATTGGATGTCACAAAG|AAC *F CCCCTCGTGATCAGGGCTGTACGTCACATGATCAATGCCAGGATACGGAAGCCTGTCGTGGCGGAAACTGTGTCAATCC *F CTGCCTGGATGCATCGCCTTGTGC *F TCGAAGCGCCCAGTGTTTGGCCCAACAACATCGCGCCATTTGCAGCTGTCCCGAGAGGACACAGGGAGATCCATTCACC *F AACTGCTACGAGCCAC|CTGAAATC *F AAGACTGGCTGCACCCACGATTCGGAATGCCAGCCGACCACGGCCTGCATCAATAAACGCTGCCAGGATCCTTGTGCCG *F AGGCCAACCCATGTGCCGGAAATG *F CCGAGTGTCGCGTACAGAACTCCCGACCTATTTGCTTCTGTCCAGCTGGTTGGGGTGGTGACCCACAAGTTCAATGCTA *F CAAAC|CTGAATGCAAGATCAACGC *F CGACTGTCCGTATGACAAGACCTGTTTGAACGAAAACTGCGTGGATCCCTGCACTCATGGCCAAGTGCGTTGTGGCAAT *F GGGGCCCAGTGCCTGGCTCAGAAC *F CACCAAGCTGTGTGCATCTGTCCAACCGGAACTCAGGGAAATCCATTCATCTCCTGCATCACTGGACACTGCCAGTACA *F ACGAGGATTGTGCGGATCACGAAG *F CCTGCGATCGATTGAATCGCGTTTGCCGACCGGTTTGCGATCAGGAGACCTGTGCCCTAAACGCCATCTGTGTGGGTCG *F TCGCCATCAGCCGCAGTGCGAGTG *F CCGACCCGGTTACCAGGGCAATCCGCATGTGCAGTGCGATATACCAGTGAAGACACCCAAGCCCCAGTGTATCCAGGAT *F GCGGACTGTCCATCGAAATTGGCC *F TGCATCAATGAGCGCTGCGCAGATCCTTGCGCCACGCCTCATGTTTGCACTCCACAACAGACTTGTACGGTTTTGGATA *F CCCTTCCAAAGCGAGCCATGGCAT *F GCAAGTGCCCCGGCGATACAGTGACGGATATCTCACGCAACTGCGTGCCCATCACTGTGCCAAAGGTCATCTCCGGCTG *F CCAGCACAATTCTGAGTGTGCCAA *F TACCGAGGTCTGTTCAAATGGAAACTGTCTGGATGCATGCCGACTGGAGAGATGTGGCGTCAATGCCCAGTGTACAGCT *F CGGGATCACTATGCTCAGTGCAAC *F TGCCCTAAGGGCTTCCAAGGCAATCCTCGCATCGAATGCTACACTACAGAAGTTGATGTTCCACGAATCCCCAATCCTG *F GCTGCTCCCGCAACGATGATTGCC *F CCAGGGACCAGATTTGTCGCAACGAGATTTGCATCAGTCCGTGTGCCGCGGATGACTGTGGAATTGGTGCCTACTGCCA *F TGTGCAGCAGCGAAAGGCCATCTG *F CCGCTGTCCACCTGGTTATACCGGTAACCCACAAGAGCGCTGCCTGCCAC|CTTCCGATGTGATACTGGTGGGCTGCAA *F GTCTAGCACCGACTGCCCCTCGAAC *F GAGGCCTGCATCAACACCCAGTGCGCCAGTCCTTGCAACTGTGGACCCAATGCCGAATGCACGGTGAAGAATCACCATC *F CGATCTGCTACTGCAAGCCTGGAT *F TCTCCGGAAATGCCCAGTTTGGATGTGCACCCATTGGCTGCCGGTCGGATGACGAGTGCAGCGGAGACAAGCAGTGTGT *F GAACCGCGAGTGTATCAATCCCTG *F CCTGGCCAGCGATCCGTGTGCCCTTAATGCCGAGTGTTACGGACGTAACCATCGCGCCAATTGCCGCTGTCCCGTGGGA *F TTGGAGGGTGATCCGTTTGTGCGT *F TGTTTACGCCTGGAATGCCACTCGGATTACGACTGTGCATCGAACCTGGCTTGCGTGTCAAACGAATGTGTTAGCCCAT *F GTGGTCAACGTAATCCGTGCGCCC *F AGAATGCCATTTGTCAGGCTCTGCAGCATCGCGCTGTTTGCCGTTGTCCGGATCAGTTGCCATTGGGTAATCCGTACGC *F TTATTGCGAACCCAGACCTGTTGA *F GCCCGTCTGCCGAGATGATGGAGATTGTCCAAGTAAGCTGGCCTGCATCGACGACAAGTGTCAAGATCCGTGCTCTGTG *F CTCTCACCATGCCATCCAACTGCT *F CAGTGCAGTGTCCTAAATAGCGTTCCAGTACGAACAATGGTTTGTGAGTGTGCCGAGTACGAGGTGCCCGATGCCAGTG *F GAGCTTGCCGTAAGATGATGCCGC *F CTAGGCTTCCTGGCTGCGAGAGCGACCAGGACTGTCCCGACCAAGAGGCCTGCATACACGCTCAGTGCCGCAATCCATG *F CAACTGTGGAACTAACGCCGTCTG *F CCAAGTGACGCAACATCGCGCCGTATGCAGTTGCCAAGATGGATTTGAGGGTAATCCCTATGCCTCCTGTCGCTCAATT *F GGATGTCGCGTCGACGGTGAGTGC *F GATTCGGGCAAGGCCTGCATCAATGGTGACTGCATCAACCCATGCCTGATCAACGATCCCTGTGGACCCAACGCCGAGT *F GCTATGTGCAATCGAACCGTGCCC *F AGTGCCGCTGCCTGAGTGGCTATCGAGGAAATCCCTACGAGCGTTGTCGTGTCATTGGCTGCAGCAGCAACAACGACTG *F TCCCACAGACAAGACCTGCCAGAA *F CGAGCAGTGCGTGAATCCCTGCGTCTACCACAACCCGTGTGCCCCGCGTGCTGAGTGCAGGGCTCAGAACCATCTGGCC *F GTGTGCCGCTGTCCTGTCGATTTC *F CTGGGCAATCCATACGTGGACTGCCGACCGCCGCCACAGCCCATCTGCCAGTTGGATACCGACTGTCCTGGTCGCCAAG *F CCTGCATCAACGAACAGTGCGTGG *F ATCCATGCGTTGTATTGGAACCGTGCCAACGGCCTGCCATCTGCGAGGTGACTCCTACATCGCCGGTGCGTACGATGTT *F ATGTATTTGTCCGGATGGTTATGT *F TAGTAGAGGAAAAGGAGGCTGCAAGCCTACGCCGGGAATCAAGGAGGTTGGAGGTTGCATCTCGGACTCAGATTGCCCG *F ACAGATAAGTCCTGCCTGAACAGC *F GTCTGTAGAGATCCTTGCAACTGTGGCTTGAATGCCGAATGCCGTATTAAGGACCATAAACCCGTATGCACTTGTCGCC *F AAGGATTCGAAGGTAACCCTGAGT *F TTGAGTGCTCCAAGATCGAGTGCAGTATCAACTCAGACTGCCCGGGAACACATGTGTGCCGCAACCAGCTCTGCATTCC *F TGCCTGCCAGGGTGAACAGTGTGG *F ATCCAATGCCCAGTGCTTGGCTATCGAGCATCGTGCGGTATGCGAGTGCATCCCAGGACATGGTGGAAATGCCAGGATT *F GCTTGTACTCCGTTGGGATGTCGC *F TCAGACGACGAATGTCCAACGGACAAGGCGTGCGTCAACGGTAAATGTAATGATCCTTGTACAACAACTGCTTTATGTG *F CCCAGGACGAGCTTTGCAAGGTGT *F ATCATCACCGACCGCAGTGCGCCTGTCCACCAGGAACCGTGCCCGGAAAGAATGGCTGCGAATCGGAGCGACACATTCC *F CATCTGCATAAGCGATGCGGACTG *F TCCCAGCCAGAAAGCCTGTCTGCGCGGAGAGTGTGTGAATCCGTGCAATGCCACCCAACCATGCGGAGTCAATGCCTTC *F TGCAGTGTCCGTGATACCTTGCCC *F GTAAGGACGATGATCTGTGAGTGCCTTGAAGGCTACACTGGCAACCCAGCCGTGCAGTGCGACAAGCGTTCGCTGTGCG *F TCATCGAGAAGGGCTTCGTTCGGG *F ATGTGGATGGACAATGTGTTTGCCCACCGGGAACCGCTTTGGATATCTACGAATACTGCACCCCATGCCGAGAGGAGCA *F AGGCTTCCGCATCGACGAGAGTGG *F TCACTGTGTGTGCGCCTTGGAGCGCGGAATGGTGATCGACGAGCGCGGCCGTTGCACTTGCCCCATTGATCTGGGCTAC *F CGACTGACTCCACGTGGCGAATGT *F CAGCCAGAGGAGCCGCCAGAGTGCACGAGCAATGATCAGTGCGCCGACAACCGATTCTGCAACCTAGACACCAAGACCT *F GTGAGGATCCCTGCCTGACCAAGG *F TGTGCGGAGTGAATGCCTTCTGTAACGCGGTGAATCACCGAGCCCAGTGCCAGTGCATCACCGGTTACACGGGTAATCC *F GGATCTACACTGCAACCACACAAA *F CTTCCGAACCGATTTCCCACGACCCGATATGGTCGTCAGTTGTCTTGCCGATGGAGTTCAAGTTGAGATCCACATCACC *F GAACCAGGATTCAATGGAGTGTTG *F TACGTTAAGGGACACAGCAAGGACGAGGAGTGCCGTCGGGTGGTTAACCTGGCTGGCGAGACTGTTCCGCGTACCGAGA *F TCTTCCGTGTCCACTTCGGTAGCT *F GCGGCATGCAGGCTGTAAAGGATGTGGCAAGCTTCGTGCTGGTCATCCAGAAACATCCCAAGCTGGTCACCTACAAGGC *F CCAGGCCTACAACATTAAGTGTGT *F CTACCAGACTGGCGAGAAGAATGTTACCCTAGGATTCAACGTATCCATGCTGACGACTGCCGGAACAATTGCCAACACA *F GGACCGCCGCCGATCTGCCAGATG *F CGCATCATCACCAACGAAGGCGAAGAGATCAACTCTGCAGAGATCGGCGACAATCTCAAGCTGCAAGTGGATGTGGAGC *F CAGCCA|CCATTTATGGAGGTTTCG *F CCCGCTCTTGTATTGCCAAAACCATGGAGGACAATGTGCAGAACGAGTATCTGGTCACGGATGAGAATGGGTGTGCCAC *F GGATACCAGTATCTTTGGAAACTG *F GGAGTACAATCCGGACACGAACAGCCTGCTGGCCAGTTTCAATGCTTTCAAGTTCCCATCGAGCGATAACATCCGCTTC *F CAGTGCAACATACGAGTCTGCTTT *F GGACGCTGTCAACCCGTCAATTGTGGTGGCTACAACGCCTTCGGGCGGCGTCGCCGCTCCATAGCGGATAACTCCACCG *F ATGCGACCGCCATTGCCACGAATT *F CGGGTGTGGAGGGTCAACTGCGCGAAGAGATCACAATCTCATCAAATGCCATTTTGACATTCGAGAAGCGCAGCGGTCA *F AGGTCTCAATGATGCCAACA|TAAA *F ACCGGCGGCTCAAAGGGTTGAGGATATCTGCGTATCTATGGTGGGGCTGATTATAGCACTGGTCATCACGGCACTGCTG *F GCTCTTGTGGCCGTTGCCGTGGCA *F GTTTCCTGCTGGCTAATGGCCTACAGGAGGAGGCCCAAGACCATTGCGCCCCTGCCCCATCCGCCAGAGTTCCCCAATC *F CGCTGTTCTCCAATCCCGACGCAG *F TGCCCGAGCCAACGCCGGATTACATATCC_TAA_ACGTAACGTAACAATACACTGATACACAACACCTAGCTATATCTT *F TAGTATGTAAGAGAATCGAATGAAAA *F TGAAAAA *F The predicted protein sequence of Dumpy: *F The sequence is split between the amino-terminal and carboxy-terminal *F halves. In between lies an additional 11 kb of dumpy coding region *F consist- ing entirely of highly conserved 306 bp tandem repeats, *F encoding the `Pigs-Feast' repeat. Because there is virtual identity *F between these repeats it has not been possible to contig this region. *F (A representative Pigs-Feast repeat at teh end of the 'Amino-terminal' *F section is flanked with __.) Dumpy represents an extreme form of *F modular protein evolution, containing 308 Epidermal Growth Factor *F modules, interspersed with a new module class DPY and terminating in a *F cross-linking Zona Pellucida domain and membrane anchor. The version *F of this figure that appears in FBrf0131182 is very helpfully color *F coded with respect to the various domains \- it is not possible in this *F version to reproduce that annotation. *F Amino-terminal half of Dumpy: *F MKIFLPLVTWIVLLLSSAVHSQYSQQPQPFKTNLRANSRFRGEVFYLNLENGYFGCQVNESTEYLQLFNLSKLCDGTQD *F CFLGADELSKEL *F KCTNDCDKDGTKCTHGACLNGVCHCNDGYGGCNCVDKDENECKQRPCDVFAHCTNTLGSFTCTCFPGYRGNGFHCEDID *F ECQDPAIAAR *F CVENAECCNLPAHFLCKCKDGYEGDGEVLCTDVDECRNPENCGPNALCTNTPGNYTCSCPDGYVGNNPYREGCQDVDEC *F SYPNVCGPGAI *F CTNLEGSYRCDCPPGYDGDGRSESGCVDQDECARTPCGRNADCLNTDGSFRCLCPDGYSGDPMNGCEDVDECATNNPCG *F LGAECVNLGGS *F FQCRCPSGFVLEHDPHADQLPQPLNTQQLGYGPGATDIAPYQRTSGAGLACLDIDECNQPDGVAKCGTNAKCINFPGSY *F RCLCPSGFQGQ *F GYLHCENINECQDNPCGENAICTDTVGSFVCTCKPDYTGDPFRGCVDIDECTALDKPCGQHAVCENTVPGYNCKCPQGY *F DGKPDPKVACE *F QVDVNILCSSNFDCTNNAECIENQCFCLDGFEPIGSSCVDIDECRTHAEVCGPHAQCLNTPGSYGCECEAGYVGSPPRM *F ACKQPCEDVR *F CGAHAYCKPDQNEAYCVCEDGWTYNPSDVAAGCVDIDECDVMHGPFGSCGQNATCTNSAGGFTCACPPGFSGDPHSKCV *F DVDECRTGASK *F CGAGAECVNVPGGGYTCRCPGNTIADPDPSVRCVPIVSCSANEDCPGNSICDATKRCLCPEPNIGNDCRHPCEALNCGA *F HAQCMLANGQA *F QCLCAPGYTGNSALAGGCNDIDECRANPCAEKAICSNTAGGYLCQCPGGSSGDPYREGCITSKTVGCSDANPCATGETC *F VQDSYTGNSVC *F ICRQGYERNSENGQCQDVDECSVQRGKPACGLNALCKNLPGSYECRCPQGHNGNPFIMCEICNTPECQCQSPYKLVGNS *F CVLSGCSSGQA *F CPSGAECISIAGGVSYCACPKGYQTQPDGSCVDVDECEERGAQLCAFGAQCVNKPGSYSCHCPEGYQGDAYNGLCALAQ *F RKCAADRECAA *F NEKCIQPGECVCPPPYFLDPQDNNKCKSPCERFPCGINAKCTPSDPPQCMCEAGFKGDPLLGCTDEDECSHLPCAYGAY *F CVNKKGGYQCV *F CPKDYTGDPYKSGCIFESGTPKSKCLSNDDCASNLACLEGSCVSPCSSLLCGSNAYCETEQHAGWCRCRVGYVKNGDGD *F CVSQCQDVIC *F GDGALCIPTSEGPTCKCPQGQLGNPFPGGSCSTDQCSAARPCGERQICINGRCKERCEGVVCGIGATCDRNNGKCICEP *F NFVGNPDLICM *F PPIEQAKCSPGCGENAHCEYGLGQSRCACNPGTFGNPYEGCGAQSKNVCQPNSCGPNAECRAVGNHISCLCPQGFSGNP *F YIGCQDVDEC *F ANKPCGLNAACLNRAGGFECLCLSGHAGNPYSSCQPIESKFCQDANKCQCNERVECPEGYSCQKGQCKNLCSQASCGPR *F AICDAGNCICP *F MGYIGDPHDQVHGCSIRGQCGNDADCLHSEICFQLGKGLRKCVDACSKIQCGPNALCVSEDHRSSCICSDGFFGNPSNL *F QVGCQPERTVP *F EEEDKCKSDQDCSRGYGCQASVNGIKECINLCSNVVCGPNELCKINPAGHAICNCAESYVWNPVVSSCEKPSLPDCTSD *F ANCPDASACR *F PDVLGVLKCVAICDAFTCPANSVCVARQHQGRCDCLNGFVGNPNDRNGCQPAQKHHCRNHAECQESEACIKDESTQTLG *F CRPACDTVKC *F GPRAVCVTNNHQAQCQCPPGPFAGDPYDPFNGCQSVPCVYNHDCPPSQMCNRMTHTCFDVCDEESCGDNAICLAEDHRA *F VCQCPPGFKGD *F PLPEVACTKQGGCAAGTCHPSAICEVTPEGPVCKCPPLFVGDAKSGGCRPDGQCPNGDADCPANTICAGGVCQNPCDNA *F CGSNAECKVI *F NRKPVCSCPLRFQPISDTAKDGCARTISKCLTDVDCGGALCYNGQCRIACRNSQDCSDGESCLKNVCVVACLDHSQCAS *F GLACVEGHCT *F IGCRSNKECKQDQSCIENKCLNPCQSANSCGPNALCSIDQHHSQCSCPEGFEGNPTPEQGCVRVPAPCLASNQCPSGHM *F CIGNQCNLPC *F TKTASCAVGERCYQQVCRKVCYTSNNCLAGEICNSDRTCQPGCDSDADCPPTELCLTGKCKCATGFIGTPFGCSDIDEC *F TEQPCHASAR *F CENLPGTYRCVCPEGTVGDGYSQPGCSQPRQCHKPDDCANNLACIHGKCTDPCLHTVCGINANCQSEGHEALCSCPAGF *F LGDPNDTGVGC *F FKVECIDHVDCAGDRACDAETNRCIKPCDLTSCGKGNCQVRDHKATCACYEGYQLVNDVCEDINECLSQPCHSTAFCNN *F LPGSYSCQCP *F EGLIGDPLQAGCRDPNECLSDADCPASASCQNSRCRSPCERQNACGLNANCQAQAHQAICTCPLNSRGDPTIECVHIEC *F ADNDDCSGEK *F ACLDSKCIDPCSLPNACGALARCSVQNHIGVCSCEAGSTGDAKLGCVQLQYCQQDGQCAQGSICSHGICSPLCSTNRDC *F ISEQLCLQGV *F CQGTCKSNSSCPQFQFCSNNICTKELECRSDSECGEDETCLSDAYGRAKCESVCLGRAACGRNAECVARSHAPDCLCKE *F GFFGDAKSGC *F RKIECTSDDDCSNDKSCDNHMCKIACLIGQPCGENALCTTEHHQQVCHCQPGFSGDPRVRCDVIDFCRDAPCGPGARCR *F NARGSYKCTC *F PPGLVGDPYNEGCRSSVECETNEDCPPHAACTKTNGVAKCRDVCAQLQCGPNAECVPKGHVAQCACRSGYDGQPADRVA *F GCKPLPSPCQ *F VTGDCPTNTYCSDSVCKPACVLDTECGAFEVCQGGQCFNPCLQPQACGQNAECVMQNHLKQCHCPEGFTGDSAKECVRV *F PVACDGECGP *F GYTCRDSMCLPVCHNDLECASNEKCLKGSCMLTCRVDNDCFLGHVCLHNKCVYGCHVDDDCSASESCRNDKCVNPCLEN *F PCGPNAACSV *F SNHRASCSCLESMVPNPTPQVGCVRSPPLECRENRDCGNGLACFESVCRPLCADDAGCLTNERCQQGVCKPLCRHDNEC *F GHGELCLGLN *F CVPGCRSDQGCPPELSCVGQQCVDPCADPTACGTNAHCQTIDHRKQCLCPEGLDGNANVACKVPRIACGRNEDCQSNQL *F CYAGSCQGKC *F RNDQNCLADERCMRGTCRTVCNTDEACAQGQICENRMCQTGCRTDLSCATDEACVNKKCQNPCRTPGQCGQCADCLVVN *F HGVQCQCPAA *F FMGDGLTGCQLPPERCHPDCECDENGAYCAPKCSRTEDCACGQQCARGKCRNKCGPKRQCTVGQLCERGACIAGCKSNG *F DCAADQSCVN *F GKCSDPCANEKACGRNALCTVSEHRMLCYCPDGYEGEPSKECVQFECRVDTDCDSNKRCDQGKCRNPCLEYGACGTNAQ *F CRVVGRKAQC *F SCPPDFFGNPTSECRPLEGGCSSKPCGENSKCTEVPGGYECACMDGCIGDAHQGCLCGGPLVNACRDQPCGLNAACHVL *F ENNQAECYCPE *F DFPNGDAYVQCYLTTPKQDCRTLGCEVGGCVRQGYEYVCQQDTEQCYSDTDCPSEKSCLQGHCSDPCTMRGVCGLNALC *F KTVLHRPRCSC *F PSCHIGRPEIECKSDPKCVAEDTDPKTKEQIPCSTDSECPETLQCGQYGQCTDPCNNPLFICESNKKCETRRHQPVCIC *F KSGFIVNEYGEL *F TCAPDKRECYRDDDCASNMACSDGKCRNPCIVPLGRAAICAENKSCEVQNHKPVCICMRDCQPSISICLRDAGCPASQA *F CRKLKCVDPC *F EFATCAPNSPCIVEDHKPICKFCPAGFIADAKNGCQKAKPGGNCTSNTDCSQAHQCGSSGKCIDPCLTSCAGGVKCVVS *F AHRVTICTCPA *F TLTNNTDSNCTSTDITVGTTTQRIETTTDFINVKYTVMQLANQTEMRTRFTDIEAENETTGPYTTTTESYKTTKQLSSN *F PETETPTTLPSR *F PTTRPFTDQTTEFTSEIPTITPMEGSTPTPSHLETTVASITSESTTREVYTIKPFDRSTPTPVSPDTTVPSITFETTTN *F IPIGTTRGQVTE *F QTTSSPSEKRTTIRVEESTLPSRSTDRTTPSESPETPTILPSDSTTRTYSDQT_TESTRDVPTTRPFEASTPSPASLET *F TVPSVTLETTTND *F PIGSTGGQVTEQTTSSPSEVRTTIGLEESTLPSRSTDRTTPSESPETPTTLPSDFITRPHSDQT_ *F Carboxy-terminal half of Dumpy: *F TTESTRDVLTTRPFETSTPSPVSLETTVPSVTSETSTNVPIGSTGGQVTEQTTAPPSVRTTETIVKSTHPAVSPDTTIP *F SEIPATRVPLES *F TTRLYTDQTIPPGSTDRTTSSERPDESTRLTSEESTETTRPVPTVSPRDALETTVTSLITETTKTTSGGTPRGQVTERT *F TKSVSELTTGRS *F SDVVTERTMPSNISSTTTVFNNSEPVSDNLPTTISITVTDSPTTVPVPTCKTDYDCLDEQTCIGGQCISPCEYFTNLCT *F VQNLTICRTLN *F HTTKCYCDTDDDVNRPDCSMKAEIGCASSDECPSQQACINALCVDPCTFNNPCSRNEDCRVFNHQPLCSAEHGRTPGCE *F HCPPGANCDPT *F TGACIKGKFYCQSFSLINLKLLALSVNTMCTYSNLKLTSRYTRVNTSLSLANVTITTITTKNSTSTKIPTKPRTTANPN *F TGVKTTPTTTRV *F TTRNTTTTTTTTTTSSTSTESSTITSATNQTSKNQKPDTESTTSHTDATRRYRDGENNITDTPTPRPTIQTTTLRGEGV *F MGDSQRRSTTTP *F KMKTTRLDTSNEVPDTTSPWPIELPTTEGTTTEVYNTMFAPVVNTTDTSLINPCTVDTNCAPNEHCKLGHCRKKEPPGS *F PKTPEPCQSNN *F DCIESEACYMGLCQDPCEFAKICAATAKCTAKSHRPVCTCPQGHEGNPMVKCVTTQTSIECTDDSDCGVTEACINQLCQ *F HPCDVHDPCA *F TNAVCINSNHAADCSCADGFQGNGFVGCQPARSHVCQYNEDCPPTKLCDRLNRRCINPCQEDSCGENAECIPVNHGTEC *F RCLPGFLGNAY *F VQCLPSQGCRSDSECDSSQACINGKCSSPCQCGAYALCDVVNHRGVCKCPPGYNGNPKVGCSPPQDPCDPNPCGLNALC *F ELDNGNPICY *F CPKGLTGNPFKNCIPEGDECTPNPCGPNSGCRRVGGNPVCFCLPEYEGQPPSIPCELPSNPCDPSPCGPNTQCSVLSNG *F FSKCTCLPNYV *F ESPNTIRGCVEPINPCDPNPCGTGAICDSSRHPVCYCPDNKIGNPFRLCDKPAVTIELCQPGPCGRNAECYVAGNREEC *F YCRSGYVGDAY *F QGCREPSRTVCDPNPCGPNANCVVAGDGQTACVCPDGLSGDPTSVIGCHGYECQVDADCPNSKACMGYRCYDPCPGACG *F QGAHCQVEEH *F HPVCSCNSGLTGNPGIRCYALDHPKKNPCVPSPCGRNSECKLLNNRAVCSCIPGYLGDPQSGCQPECDINSDCGDTLSC *F INHKCVDPCA *F GAICGINAICNVRQHTPVCLCLDGFVGDAFLQCVPIGILKNVSRDPCAPSPCGPHDVCSVYGDGVALCDPCFGPNAQQN *F PRCRPECVGNS *F DCPFDRACLGQRCLDPCPGSCGRNAICNVYEHNPVCACPTGLFGNPYEQCTTKSVVETPPQPSCAKLHCGANAECKRQH *F SGLACVCRKGY *F FGDPHIGCRPECVLNSDCPAEKACLNSKCVEACTGVCGVNAVCRVVNHAPVCICAEGYSGDASIACNPFYLPPPERPHP *F CEPSPCGPNS *F RCKATPDGYAACSCLPNFKGAPPVCQPECVVSSECAPNQACLNQRCTDPCPGICGGGARCEVLNHNPICSCEANFEGDP *F FVACSPIQDPG *F RDIPVPKNPCVPSPCGPNSICQIKQNRPVCSCVANYIGSPPYCRPECTLSSECPSDKACINEKCQNPCANVCGHNARCT *F VIAHSAHCSC *F DEDYEGDAFIGCSKKITERPGDHIDPCYPNPCAENAVCTPYNNAARCTCIEPYNGDPYSTGCRPECIYSSECPSSLACI *F KQHCRDPCTA *F ACGANAECTVVNHLPSCSCTRGFEGNPFDGCKRVVVVRPETVCEPNPCGPNSICRSVEGHPTCSCQVGYFGAPPQCRPE *F CVVSSECAQHL *F SCINQKCMDPCVGTCGFNAKCQVNNHNPICSCPANYEGNPFEQCMPKPAEPTRNVDPCLPSPCGSNSICRNVNNRAECS *F CAPGMFGAPPN *F CRPECVINQDCPSNRACIRQRCEDPCIGICGFNAVCSTQNHQPKCSCIESFEGDPYTACKMREIVVLDPPTDPCYPSPC *F GANAICRVRN *F GAGSCSCIQNYFGDPYINCRPECVQNSDCPNNRACINMKCRDPCANACGFNAICRVAHHQPVCSCEPHLTGNPLRACVE *F RPSNMYLPLP *F KDPCRPSPCGLFSTCHVVGERPVCACLPDYMGAPPNCKPECMTSAECPSDRACINQRCKDPCPGTCGYNARCRCTNHSP *F ICSCYDGYTG *F DPFHQCVPERKPPPIADPIVPPNPCVPSPCGPNSQCQVSSSGAVCSCVTNYIGRPPGCRPECSINSECPARMACINARC *F ADPCIGSCGN *F NALCHVSLHAPVCMCEPGYSGDPFSGCYKIIETPIEVIQPCRPSPCGLNALCEERNQAAACKCLPEYFGDPYVECRPEC *F VINSDCPRSRA *F CVNQKCVDPCPGMCGHNALCAVFNHAPNCECLPGYTGNPIVGCHIVPESPRYPDPIVPENPCQPSPCGLYSNCRPVNGH *F AVCSCVPSYIG *F SPPNCRPECMSSSECAQDKSCLNERCKDPCPGTCGNNALCRVVNHNPICSCSPGFSGDPFVRCFPQEKRPPITHDRIDP *F CVPSPCGPNS *F ECRVSAANEQAVCSCLQHYVGRAPNCRPECTSDSECPGNLACINLRCRDPCVGTCGIQTTCLVNNHRPICRCIDGYAGD *F PFSECSPKINV *F PVQVAQPCNPSPCGANAVCKERNGVGSCSCLPEYNGDPYTECRPECVLNSDCSKNRACLNNKCRDPCPGVCGVSAECHV *F INHAPSCSCP *F SGFTGNPSQFCREIPRLPAPVEPCRPSPCGPYSQCREVNGHAVCSCVTNYIGTPPACRPECSVSSECAQDRACVNQRCA *F DPCPGTCGNE *F AICKVTNHNPICSCPAGYSGDPFVRCAPWQEEPEQPKSNENPCVPSPCGRNSQCRVVGETGVCSCLPNFVGRAPNCRPE *F CTINTECPANL *F ACINERCQDPCPGSCGFNAFCSVVNHSPICTCDSGYTGDPFAGCNPQPPAIPDERLTPCQPSPCGPNAECRERNGAGSC *F TCLPEYFGDPY *F SGCRPECVVNSDCSRDKSCVNQKCVDPCPGVCGLNAQCRVSNHLPSCSCLAGYTGNPSSACREIPQLPPPPERDENPCR *F PSPCGPYSQC *F REVDGHAVCSCLQGFIGSAPNCRPECIISSDCAQNLNCQNQKCVDPCPGTCGIEARCQVINHYPACSCAPGFTGDPFNR *F CTKILLEPPPT *F EKSGNPCIPSPCGPNSKCLDVRGSPACSCLPDYLGRPPNCRPECLSSADCPANLACVNQRCSNPCIGACGLHSVCTVIK *F HRPACECVPGY *F TGDPFSGCAIVQQIAPPDETRNPCNPSPCGANAICRERNGAGSCACLPEYFGDPYSGCRPECVQNDDCDRSRACINNKC *F QDPCPGACGI *F NAECRVLNHGPNCNCFDGYTGDPHRSCSLIEVVTIRPEPCKPSPCGPYSQCLDTNSHAVCSCLEGYIGAPPSCKPECVV *F SSECPQNRACI *F NQKCEDPCRGSCGNNAKCQVVNHNPICTCQPGMTGDPISGCEPMPEVKNVENPCVPSPCGPNSVCRQIGNQAACSCNAG *F YIGRPPTCRP *F ECTNNDECQNHLSCQQERCVDPCPGSCGSNAICQVVQHNAVCSCADGYEGEPLFGCQLIPAVTPTESPSSPCEPSPCGP *F HAECRERNGAG *F ACYCHDGFEGNPYDAQRGCRRECENNDDCTAVQACSRFKCVDPCNNICGDYAICTVDKHVPTCDCPPGYTGDPFFSCKP *F VPVTPRPPLNP *F CNPSPCGPNSNCRAMNNQAVCSCQAGFINQPPNCKPECVVSAECAPEKACVHKKCVDPCQHTCGIRAICTTKNHSPICT *F CPRTMTGDPFV *F ECTRVAITNDNTTPSPAPASCVPSPCGPNAKCQIVGNSPACSCLPNFIGAPPRCRPECVLNSECGPTEACINQKCADPC *F SGSCGFEAKC *F HVLNHLPICNCIEGYEGDPFVRCTKKEEDRSPPPPNDPCNPNPCGQNADCFAGECRCQNNYQGNAYEGCRPECTLSADC *F PRDKACMRNRC *F VDPCPGICGNNAVCEVMNHIPVCSCVKGYEGDPFVNCRVKPVVEDPIIEACSPSPCGSNSQCRDVNGHAVCSCLEGYIG *F APPQCRPECV *F VSSECSALQACVNKKCVDPCAAACGLEARCEVINHSPICGCPPGRTGDPFKQCVVLPPIAVPDVKSPPQDPCVPSPCGP *F NSICKNDRNGP *F VCQCQPEFFGSPPNCRPECIINPDCQSTQACINNKCSNPCPESCGTNAECRVIGHAVSCSCPTGYAGNAFVQCVPQQEE *F PPKPCQPSPC *F GPNAECIERNGAAACKCIDEYQGNPYEGCRPECVLSSDCPTDKTCIRNKCQDPCPGICGLNAQCYAVNHVPNCVCNDGY *F TGDPFASCRRV *F EVTTPSPVSDPCIPSPCGANSKCRVANGLAVCSCMETFIGAPPNCKPECTVNAECPSNRACHKFRCANPCAKTCGLNAK *F CEVINHNPIC *F SCPLDMTGDPFARCYPAPPPPPPGPKDEPVRRPCQPSPCGLNSECRVRDEQASCSCLPNFIGAPPNCRPECVVNTDCSP *F DQACIAEKCR *F DPCDGSCGVDSECRVQNHLAICTCRGGFTGDPFVRCFEFVEETTKSPPLTQDPCDLQPCGSNAECRNGICSCLADYQGD *F PYTGCRPECTL *F STDCAPTKACLNKKCVDPCPGVCGQNSQCDVSNHIPICSCLQGYTGDPFVHCRHETPVAKDPCQPNPCGPNSLCHISGQ *F GPVCACQPGM *F LGSPPACKPECIVSSECSLHTACVNRKCVDPCPGACGQFARCQVINHNPSCSCNTGYTGDPFTRCYQEERKPPTTPDNP *F CQPSPCGPNS *F ECKVLNGNAACSCAATFIGTPPSCRPECSINPECPPTKACIRQKCSDPCVNACGFNARCNVANHQPICTCDVGYTGDPF *F TGCQKEQERIV *F NEQVTPCEPNPCGSNAVCRERNGIGSCQCLPDHFGDPYQSCRPECVRHSDCASNKACQQQKCRDPCPGTCGSNADCSVT *F NHLPTCTCRI *F GYTGDPYRYCHVEPPQLPARVTEPSQPCRPSPCGPNSQCRELNGQAVCSCLELYIGLPPNCRPECVLSTECPTDKACIS *F QRCQDPCPGT *F CGINAECRVRNHSPLCQCRQGFTGDSFTRCYPLPPPPPVIERVERDPCLPSPCGLNSQCRNVQGVPSCTCLPDFLGAPP *F NCRPECTISAE *F CPSNLACIRERCIDPCPGSCGYAAECSVVNHTPICVCPAGFTGDPFSSCRPAPPPEPTQSEYVDPCNPSPCGPNAQCNA *F GICTCLAEFH *F GDPYSGCRPECVLNSDCPRDKACHSSKCVNPCPGTCGENAICDVINHIPMCRCPERTAGSAFIRCSPVQITVSNPCRPS *F PCGPNSQCRE *F VNQQAVCSCLPSFIGAPPSCRPECTSNSECAPTQACLNQRCGDPCPGTCGVGANCAVVSHSPFCTCPERFTGNPFIRCQ *F PQIEPPVRDVA *F PVDPCRPSPCGPYSQCRPVGEAPACSCVETYIGRPPNCRPECVTSSDCSSQLACVNQKCVDPCPGRCGLNAECFVVSHA *F VQCICQQGFN *F GDPFVQCKPEIAYENEIRTPCSPSPCGPNAVCRDRNGVGSCQCLPQYFGDPYEGCRPECMLDSDCPSNRACQQLRCQDP *F CPGTCGLNAN *F CQVVNHLPTCTCLTGYVGDPYRQCNRLPEPPQNEYVNPCQPTPCGPNSQCRVSNEQAVCSCLPLFVGTPPSCRPECTIS *F SECSADRACVN *F QKCVDPCAADTCGNNAICRVRNHSPICSCISGYTGDAFTRCFLIPPPIIETKDEPLRDPCIPTPCGPNSECRNINGVPA *F CSCLVNFIGQA *F PNCRPECTINSECPSQLACINQKCRDPCPGACGQNAVCSVINHTPLCACIDGYIGNPFTNCNPKPPEPPAPPVADDPCN *F PSPCGANAQC *F RNGQCSCIPEYKGDPYVSCRPECVLNTDCPRDRACVRNKCIDPCSGTCGVNALCEVNNHIPICRCPEQMSGNAFFECRP *F VPPAKIQNPC *F QPSPCGPNSQCRVVQQTAVCSCLANYVGSPPQCRPECVTNSDCPADQDCQNMKCRDPCPGTCGFNALCNVVNHRPFCSC *F PTGMSGNPFVS *F CQQLIIRDERPQNPCQPSPCGPNSECRVSGDSPSCSCLPEFVGAPPNCRPECISNSECPTNQACINQKCVDPCPGLCGQ *F NAICRVFSHS *F AMCLCDGGFTGDPFSQCSPIRDSPPEVLQPCNPSPCGVNAKCEERGGAGSCQCLPDYFGNPYDGCRPECVLNSDCPSNQ *F ACVNQKCRDP *F CPGTCGQNAECQVVNHLATCNCLVGYTGDPYSICRITVNEPPERVYVNPCQPSPCGPNSQCREVNEQGVCSCLPEFIGS *F PPACRPECTSS *F SECAADKACVNRKCVDPCPNVCGQQAECRVRNHNPICTCLSGFTGDPFTRCYRQPPPPPVVEREPLDPCVPSPCGANSQ *F CREIHGTPSCS *F CLPQYLGTPPNCRPECSINAECPSHQACINQKCRDPCPGSCGLNTQCSVINHTPICSCLAGYIGDPFSVCNPEPIPEKI *F RDPLPPEDPC *F NPSPCGSNTQCNNGVCSCLPEYHGDPYTGCRPECVLHTDCDRSRACVRHKCVDPCPGTCGTNAICEVLNHIPNCRCLEG *F MQGNAFIQCSP *F VPKLDVVQNPCQPSPCGPNSQCRVVNQQAICSCITSFIGSPPFCRPECTTNSECPLNLACRNQKCSDPCPGVCGRGAQC *F HVTNHSPFCR *F CLERYTGNPFVSCQQIIEPPVPPPRQTCLPSPCGPYSQCREVNESPSCTCLPEYIGAPPNCRPECVTSSECPTNQACIQ *F QKCRDPCPGL *F CGQSAECRVLSHTPSCVCPEGMEGDPFTLCKEKRIQELDQLDPCSPSPCGINARCTSRQDAGSCQCLPDYFGNPYEGCR *F PECVLNSDCPS *F NKACQQQKCQDPCPGTCGQNALCNVLNHIPSCSCISGYSGDPYRSCVPEPVKEYVNPCQPSPCGPNSQCREVNEQAICS *F CLPEYVGAPPV *F CRPECTISSECPADKACVNQKCVDPCPNTCGDQAICRVVNHSPICSCRAGYTGDAFFRCFPKPPVPPTPVQKTPVDPCV *F PTPCGPYSQC *F RSQGDAPACSCLVGYIGAPPNCRPECRINAECPSSQACINEKCRDPCPGSCGYGAICNVINHTPSCTCPPGYSGDPFSQ *F CQPVPPPPPTP *F VKLDDPCNPSPCGPNAQCNNGVCTCIPEYHGDPYSGCRPECITSADCSRELACSRNKCFDPCPGTCAPNAICTVLNHVP *F MCTCPEGYNG *F NAFVQCKPTPRKYFPSRPCSKPNDTQLNDFLPAPALVQPCQPSPCGPNSQCREVNQQAVCSCVPGYIGTPPLCRPECTS *F NSECLSHLACV *F NQKCNDPCPGSCGRNAQCSVVNHNPFCTCLPRFTGNPFVGCQQIIEPPRQDIVPQDPCRPSPCGPNSECRAAGETATCT *F CLGDFVGSPPY *F CKPECVANSECPSNLACINQKCRDPCPGLCGSSATCRVVSHTAMCICDAGLTGDPFTQCQPIVQDVEIINPCQPSPCGA *F NAECIQRNGA *F GACQCLTDYFGNPYEGCRPECVLNSDCPSNRACQQQKCRDPCPGSCGQNAECNVVNHTPMCNCFAGFIGDPYRYCSQPP *F EPIVHEYVNP *F CQPSPCGPNSNCREVNEQAVCSCRSEFEGAPPNCRPQCTSSSECASNRACINQKCVDPCPGVCGQQAICEVRNHSPICR *F CPTAMIGDPFV *F RCIPRPTIAPPPLRDVAPYRDPCLPSPCGLYASCRNQQNQAVCSCLPNYFGTPPHCRPECSINAECPSHLACIGERCRD *F PCPGACGQQT *F ECRVISHVPSCVCLRGYVGDAFLACHPAPPPPSREEPRDPCNPSPCGSNAICSNQGECKCVADYQGDPYVACRPECVLS *F SECPRNLACIQ *F QKCTDPCPGTCGTNAICDVVNHIAMCHCPDRMTGNAFVQCTPVQLDVYRNPCNPSPCGSYAECREQNGQAVCSCLPNYF *F GVPPSCRPEC *F STNYDCSPSLACQNQRCVDPCPGACGAYAECRTVNHSPFCSCRPGYTGNPIVQCHMIIEPQRDITPKDPCQPSPCGPNS *F ECRRVGETPSC *F SCLSNFFGTPPNCRPECVSNSECSQVHVCSNNRCKDPCPGLCGTDAVCRVISHSAMCYCQPGYSGDPFVRCAPHIQRES *F IEIVQPCNPN *F PCGAFAECRQQNGVGSCQCLPEYFGNPYEGCRPECVLDSDCPSQLACVNQKCRDPCPGSCGQNAECFVRNHLPTCNCLS *F GYVGDPYRYCS *F IEPKPIREYVNPCQPSPCGPNSQCREQNGVATCSCLPEFVGTPPGCRPECTVSSECNLDKACVRHKCLDPCPGACGSSA *F NCQVVNHAPL *F CSCQAGYTGDPFTRCYPIPSPPTHIVHDYARHPCQPSPCGANAQCRQSQGQAICSCIPNYFGVPPNCRPECTQSSECLS *F SLACINQRCA *F DPCPGSCAYNAICHVRNHVPSCQCPVGYVGDPFTNCHPEPQPPPKPVALDDPCNPSPCGANAVCQNGQCSCIPEYQGDP *F YTGCRPECVLN *F ADCPRNRACVRHKCVDPCPGTCAPNAICDVINHIAMCRCPERMTGNAFIQCETPPVSLAPPDPCYPSPCGPNSRCRVFN *F NNAVCSCIEDF *F IGTPPNCRPECTHNSDCLPRLACQRQHCIDPCPGTCGFNALCHVVNHAPICSCPPKHNGNPFLGCFPEPVRRDEVIPKN *F PCQPSPCGPY *F AKCTSVGDQAQCSCLPEYIGTPPNCRPECITNSECSFDKACLNQRCRDPCSGTCGSNANCHVISHTAMCYCLPGFTGDP *F FTSCVQVPVIQ *F QAEIVQPCSPNPCGANAVCRQEGHVGSCQCLPEYYGNPYETCRPECVTNNDCPSNKACQQQKCRDPCPGVCALNALCRV *F INHLPTCHCQ *F NGFVGDPYRYCQIPEKPVLKEYINPCQPSPCGPNSQCRENNEQAICSCLPEYVGAPPNCRPECVTSAECPHDKACIRQK *F CNDPCPGVCG *F SNADCRVIQHAPICSCRAGFTGDAFSRCLPLPPSRPPQLDVYRNPCVPSPCGQYAECRDNQGTATCSCLPSYFGTPPNC *F RPECTINPDCP *F SHLSCQQQRCRDPCPGACGFNALCTVINHNPTCQCAPGFIGNAFTSCHVPPPIVRDPPQISDPCDLITCGPNAVCNQGQ *F CNCLPEFVGNP *F LVGCRPECVLSTECDWSKACVRNKCIDPCPGTCGSNAICEVHRHIAMCHCPPEMTGNAFSQCRPLPPAPVRDVIDPCQP *F SPCGPNAQCR *F NINGQAVCSCLRDFIGVPPSCRPECVSNAECPLHLACLQRHCRDPCPGVCGLNAECRVINHSPNCHCIGSFTGNPFAAC *F HRPPPPPIKHE *F PIDPCQPSPCGANAECRVQGSNAQCSCLSGFIGTPPNCRPECVSNSDCPMNLACLNQKCRDPCPGVCGSNAECYVINHT *F PMCTCLAGQT *F GNPFVSCQVVRDVPEPQTPCVPSPCGANALCSEGNGAGACKCLPEFYGNPYEGCRPECVLNSDCPSHLACLNQHCRDPC *F PGTCGINAEC *F QVRDHLPQCNCHVGYQGNPYVYCSVLRDPLPEPVPSRPCQPSPCGPNSQCRESNNQAICKCLPNFIGSPPACRPECTIS *F SECDLTLACVQ *F QHCVDPCPGVCGNSAQCRVINHSPHCSCLPGFTGDAISGCQRIPPAITHDAPNETPRDPCVPSPCGAFGQCRAQGNQAI *F CSCLPGYYGAP *F PNCRPECAINPDCASHLACISEKCRDPCPGSCGLQAQCSVINHTPICSCPSGYEGNPFVRCQRTPPTPTPPLHDACNPS *F PCGSNAICSP *F GGQCSCLPDFDGNPYVGCRPECVLNTDCARDKACQRSKCTDPCPGACGIGAVCEVRNHIPTCNCPPGTSGNAFVQCTLV *F QSSPVVPLNP *F CQPSPCGNNAQCREVNDQAVCSCLPGFFGVPPKCRPECTINSDCAPHLACLNQQCRDPCPGACGQFAQCQVIRHVPHCS *F CPAGFSGNAFF *F LCQRLPPPPPVQREPINPCYPSPCGPNAECTNQNEQAICKCLKDYIGTPPNCRPECITSSECPIQLACIGQKCKDPCSG *F LCGIAATCQV *F VSHVPSCICIADYIGDPYTGCYARPPIQREQINPCYQNPCGSNAVCRERGEAASCQCLPEYYGNPYEGCRPECVLNSDC *F SSHLACLNQHC *F RDPCPGSCAPNAQCQVVNHVPSCSCYPGYSGDPYRHCHVAQAEPVQVVHFNPCQPSPCGPNSQCTESQGQAVCRCLPDY *F YGSPPACRPE *F CTTNPECPNDKACVSRRCTDPCAGACGQNAICRAHQHRAYCSCHPGYTGDAFMRCQSLPSPQPIRDSPVIYRDPCVPSP *F CGQFAQCRVEY *F EQAVCSCLTSYYGTPPYCRPECTQNSDCPSHRACVNQRCVDPCPGACGLNARCDVLNHVPSCSCPEGYLGDPFYRCYPA *F PAPPPTPVTV *F VADDPCQPSPCGPNAQCSNGVCSCLPLYQGDPYVGCRPECVLSTECPWDKACIRNRCLDPCPGTCGSGATCQVHNHVAM *F CQCPVGYQGNP *F FVLCQQTPLQAPVELHPCQPSPCGHHGECREVGSQAICTCRLGYYGSPPACRPECVSDPECPPSLACVNQKCRDPCPGA *F CGHLAQCHVI *F NHSPQCVCPAGYTGSPYSECHLIRADSSPIQRQPIDPCLPSPCGPHAQCSNEGGNAVCRCLTEYLGVPPYCRPECIANS *F ECPSDRACINR *F KCQDPCPGLCGYNAICRTYNHQPNCVCAPGLVGNPFNSCLPPTRPEIPATPPTTAIQVLQYEEPFINGCEPNPCGANAQ *F CNQRRGVVSCV *F CLPDYFGNPYEACRPECILNSDCPLSRACVQQKCRDPCPGTCGLNAECHVMDHLPQCRCFSGYTGNPLAYCSPVPIIQE *F SPLTPCDPSP *F CGPNAQCHPSLNEAVCSCLPEFYGTPPNCRPECTLNSECAYDKACVHHKCVDPCPGICGINADCRVHYHSPICYCISSH *F TGDPFTRCYET *F PKPVRPQIYDTPSPPYPVAIPDLVYVQQQQPGIVNIPSAPQPIYPTPQSPQYNVNYPSPQPANPQKPGVVNIPSVPQPV *F YPSPQPPVYDVN *F YPTTPVSQHPGVVNIPSAPRLVPPTSQRPVFITSPGNLSPTPQPGVINIPSVSQPGYPTPQSPIYDANYPTTQSPIPQQ *F PGVVNIPSVPSP *F SYPAPNPPVNYPTQPSPQIPVQPGVINIPSAPLPTTPPQHPPVFIPSPESPSPAPKPGVINIPSVTHPEYPTSQVPVYD *F VNYSTTPSPIPQ *F KPGVVNIPSAPQPVHPAPNPPVHEFNYPTPPAVPQQPGVLNIPSYPTPVAPTPQSPIYIPSQEQPKPTTRPSVINVPSV *F PQPAYPTPQAPV *F YDVNYPTSPSVIPHQPGVVNIPSVPLPAPPVKQRPVFVPSPVHPTPAPQPGVVNIPSVAQPVHPTYQPPVVERPAIYDV *F YYPPPPSRPGVI *F NIPSPPRPVYPVPQQPIYVPAPVLHIPAPRPVIHNIPSVPQPTYPHRNPPIQDVTYPAPQPSPPVPGIVNIPSLPQPVS *F TPTSGVINIPSQ *F ASPPISVPTPGIVNIPSIPQPTPQRPSPGIINVPSVPQPIPTAPSPGIINIPSVPQPLPSPTPGVINIPQQPTPPPLVQ *F QPGIINIPSVQQ *F PSTPTTQHPIQDVQYETQRPQPTPGVINIPSVSQPTYPTQKPSYQDTSYPTVQPKPPVSGIINIPSVPQPVPSLTPGVI *F NLPSEPSYSAPI *F PKPGIINVPSIPEPIPSIPQNPVQEVYHDTQKPQAIPGVVNVPSAPQPTPGRPYYDVAKPDFEFNPCYPSPCGPYSHCH *F NRFGVAACVCL *F PNYRGTPPNCRPECVINSDCPSSLACINEKCRDPCPGSCAYNAVCRVHEHVPNCYCQTGYTGNPFISCQRTPIAPVQRE *F PIEAKDPCYP *F SICGPNAVCNNGKCSCIPEYRGDPYVGCRPECVLNTDCARDKACIQQKCKNPCPGTCGLQALCHVYNHVATCSCPEGMQ *F GDAFVRCDPKP *F KPQPPAPAPPTTLPAIVPQRAPINPCQPTPCGPNSQCRAYHEQAICYCLPNFIGTPPGCRPECTSNSDCPLDKYCLNLR *F CRDPCPGACG *F IRAICHVQNHGPLCVCPPHLTGNPLLACQPIVIPPVERDEVNPCQPSPCGPNSECQATSGGARCSCLPQYHGTPPFCRP *F ECVNSADCPAD *F KACRNYKCIDPCPGSCGFSALCRVVAHSPVCYCPEGYVGNAYTLCSRPEPSPPAVVILPCNPSPCGVNAFCQPHNDLSV *F CQCLPGYYGNP *F SEICRPECTVNSDCPSHRACMSEKCRDPCPGVCGLNALCQVINHSPVCECHTGHVGNPYHSCRIPQREPPAPEYVNPCQ *F PSPCGANSQC *F RESQGQAICSCLPEFVGTPPSCRPECVISAECPADRACINQKCQDPCPGACGLNAQCHVRNHSPLCSCQPGFTGDALTR *F CLPVPPPQPPK *F SNDIRDPCVPSPCGPYSQCRVVNGGASCSCLPNYVGAAPNCRPECTINAECPSNLACINEKCRDPCPGACGFAAQCSVI *F NHTPSCSCPA *F GYTGDPFTSCRVLPPPPPPKTPSDPCQPSPCGANALCNNGQCSCLPEYHGDPYTGCRPECVLNSDCPRNRACVNQKCVD *F PCPGHCGLNA *F LCDAVNHIAMCHCPERMTGNAFVSCQPIRDDPPPPTTPNPCQPSPCGANAQCLERNGNAICSCLAGYFGQPPNCRLECY *F SSSDCSQVHSC *F INNKCVDPCPGKCGLNAVCQAIQHRAHCECIPRYTGNAFVQCNPIPVPRVPEPVRDPCQPSPCGPNSQCTNVNGQAECR *F CLQEFQGTPPN *F CRPECVSHDECANTLACMNQKCRDPCPGSCGQSAQCTVSLHIPNCQCPVGMTGDPFRICLPKPRDEPKPPPTPKNPCYP *F SPCGTNAVCR *F VQGENYVCECSQLEYIGNPYEGCRPECVGNSECPANQACIRSKCQDPCPGVCGLEAICTMNNHIPICSCPPGYTGNAFA *F QCTRQVTPPPP *F SDPCYPSPCGPNSICRIQNEKAVCECLPGFFGNPLAQGCRPECTLSSDCAKDRACINSKCVDACVGECGFGAVCQTINH *F SPVCSCPANM *F VGNPFVQCEEPRQAEPIDPCQPSPCRSNGICRVYNGAATCSYPECVINEDCSRDRACVSQKCRDPCLNACGINAICRAI *F NHKAVCSCPPE *F FYGSPYAQCLRQLPEPEPKPECISDGDCTNDKACINQVCRNPCEQSNICAPQARCHVQLHRPLCVCNEGYTGNALQNCY *F LLGCRSDGEC *F AANEACVNQQCVDPCGFTQCGTGAICRADFNHRARCHCLDGYRGNPLVRCERPECRSDDECAFHLACRNERCEDPCNCG *F IGAQCRVENH *F RAQCRCPAGFSGNPAVRCDLVPTQPEGCTMDAECPSKLACFGGECKNPCDVTHPCGANAICEVVDTLPLRTMMCSCLPG *F YVGEADIGCHK *F EPPRDQGCTSHDQCQDTEACRGGNCVNPCLDASPCARSAQCLAQQHRAICSCPERTQGDPFTNCYEPPEIKTGCTHDSE *F CQPTTACINK *F RCQDPCAEANPCAGNAECRVQNSRPICFCPAGWGGDPQVQCYKPECKINADCPYDKTCLNENCVDPCTHGQVRCGNGAQ *F CLAQNHQAVCI *F CPTGTQGNPFISCITGHCQYNEDCADHEACDRLNRVCRPVCDQETCALNAICVGRRHQPQCECRPGYQGNPHVQCDIPV *F KTPKPQCIQD *F ADCPSKLACINERCADPCATPHVCTPQQTCTVLDTLPKRAMACKCPGDTVTDISRNCVPITVPKVISGCQHNSECANTE *F VCSNGNCLDA *F CRLERCGVNAQCTARDHYAQCNCPKGFQGNPRIECYTTEVDVPRIPNPGCSRNDDCPRDQICRNEICISPCAADDCGIG *F AYCHVQQRKAI *F CRCPPGYTGNPQERCLPPSDVILVGCKSSTDCPSNEACINTQCASPCNCGPNAECTVKNHHPICYCKPGFSGNAQFGCA *F PIGCRSDDEC *F SGDKQCVNRECINPCLASDPCALNAECYGRNHRANCRCPVGLEGDPFVRCLRLECHSDYDCASNLACVSNECVSPCGQR *F NPCAQNAICQ *F ALQHRAVCRCPDQLPLGNPYAYCEPRPVEPVCRDDGDCPSKLACIDDKCQDPCSVLSPCHPTAQCSVLNSVPVRTMVCE *F CAEYEVPDASG *F ACRKMMPPRLPGCESDQDCPDQEACIHAQCRNPCNCGTNAVCQVTQHRAVCSCQDGFEGNPYASCRSIGCRVDGECDSG *F KACINGDCI *F NPCLINDPCGPNAECYVQSNRAQCRCLSGYRGNPYERCRVIGCSSNNDCPTDKTCQNEQCVNPCVYHNPCAPRAECRAQ *F NHLAVCRCPVD *F FLGNPYVDCRPPPQPICQLDTDCPGRQACINEQCVDPCVVLEPCQRPAICEVTPTSPVRTMLCICPDGYVSRGKGGCKP *F TPGIKEVGGC *F ISDSDCPTDKSCLNSVCRDPCNCGLNAECRIKDHKPVCTCRQGFEGNPEFECSKIECSINSDCPGTHVCRNQLCIPACQ *F GEQCGSNAQC *F LAIEHRAVCECIPGHGGNARIACTPLGCRSDDECPTDKACVNGKCNDPCTTTALCAQDELCKVYHHRPQCACPPGTVPG *F KNGCESERHI *F PICISDADCPSQKACLRGECVNPCNATQPCGVNAFCSVRDTLPVRTMICECLEGYTGNPAVQCDKRSLCVIEKGFVRDV *F DGQCVCPPGTA *F LDIYEYCTPCREEQGFRIDESGHCVCALERGMVIDERGRCTCPIDLGYRLTPRGECQPEEPPECTSNDQCADNRFCNLD *F TKTCEDPCLTK *F VCGVNAFCNAVNHRAQCQCITGYTGNPDLHCNHTNFRTDFPRPDMVVSCLADGVQVEIHITEPGFNGVLYVKGHSKDEE *F CRRVVNLAGETV *F PRTEIFRVHFGSCGMQAVKDVASFVLVIQKHPKLVTYKAQAYNIKCVYQTGEKNVTLGFNVSMLTTAGTIANTGPPPIC *F QMRIITNEGEEI *F NSAEIGDNLKLQVDVEPATIYGGFARSCIAKTMEDNVQNEYLVTDENGCATDTSIFGNWEYNPDTNSLLASFNAFKFPS *F SDNIRFQCNIRV *F CFGRCQPVNCGGYNAFGRRRRSIADNSTDATAIATNSGVEGQLREEITISSNAILTFEKRSGQGLNDANIKPAAQRVED *F ICVSMVGLIIAL *F VITALLALVAVAVAVSCWLMAYRRRPKTIAPLPHPPEFPNPLFSNPDAVPEPTPDYIS # *U FBrf0153944 *a Baugh *b L.R. *t 2002.5.30 *T personal communication to FlyBase *u A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation. *F P{EPgy2} is similar to P{EPg}. P{EPg} was first described in a *F publication by Mata et al. (2002) Cell 101: 511-522 (FBrf0128569) and *F the FlyBase ID for P{EPg} is FBtp0012862. The major differences are *F that P{EPgy2} contains an intronless yellow gene module and lacks the *F plasmid rescue module of P{EPg}. I constructed the plasmid pP{EPgy2} *F from two plasmid precursors, p1462 and yellow-BSX. *F *F 1) p1462 was obtained from Pernille Rorth (EMBL, Heidelberg). This *F was an intermediate used in the construction of pP{EPg}. She *F described p1462 to me as being the same as pP{EPg} except for the *F absence of the plasmid rescue module. *F *F 2) The yellow-BSX plasmid was obtained from Tim Parnell in the *F laboratory of Pamela Geyer (University of Iowa). It was described as *F having the SalI fragment of yellow-intronless inserted into the SalI *F site of a modified pBluescript vector, pBS-X. This vector has the *F KpnI site of the polylinker converted into an XbaI site. This yellow *F SalI fragment is the same as segment designated by FlyBase as *F y+mDint25.2(S,S) (FlyBase ID FBms0003824). *F *F I digested yellow-BSX DNA with a combination of NotI and PspOMI. *F PspOMI cleaves the same recognition sequence as ApaI (GGGCCC), but *F generates the same 5' overhang as NotI (GGCC). NotI cuts yellow-BSX *F at a single site in the polylinker sequences closest 3' end of the *F yellow gene and PspOMI cuts yellow-BSX at a single site in the *F polylinker sequences closest to the 5' end of the yellow gene. I *F gel-purified the 5.8 kb fragment containing the yellow gene. *F *F I ligated the NotI - PspOMI fragment of yellow-BSX with DNA from *F p1462 that had been cut by NotI. p1462 has a unique NotI site *F located between mini-white and the GAGA/GAL4-UAS modules. The yellow *F fragment can insert in either of two orientations into the NotI site *F of p1462. I recovered transformants in both orientations and named *F them pP{EPgy1} and pP{EPgy2}. *F -- *F ------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu # *U FBrf0154285 *a Ashburner *b M. *t 2003.2.5 *T personal communication to FlyBase *u *F \*g S75832 best bet is bit of a R1 element by BLAST *F *F >EM_INV:DMRGM219 K01582.1 d.melanogaster 28s rrna gene, 5 kb type i insertion, clone *F cdm219, units 3 and 4 of tandem array. *F Length = 612 *F *F Score = 93.7 bits (47), Expect = 7e-18 *F Identities = 53/55 (96%) *F Strand = Plus / Plus *F *F *F Query: 3 aaaagcatacattgtccctatctacgcgcatcaatggattaacgacggacgtgtt 57 *F |||||||||||||||||||||||||||| || ||||||||||||||||||||||| *F Sbjct: 215 aaaagcatacattgtccctatctacgcggatgaatggattaacgacggacgtgtt 269 *F *F *F \# *F \*g S75831 rDNA spacer by BLAST *F *F >EM_INV:AF191295 AF191295.1 Drosophila melanogaster 28S ribosomal RNA gene, partial *F sequence; and external transcribed spacer, complete *F sequence. *F Length = 1335 *F *F Score = 178 bits (90), Expect = 5e-43 *F Identities = 124/133 (93%), Gaps = 8/133 (6%) *F Strand = Plus / Minus *F *F *F Query: 28 gataaaattctttaaattctttatattaattatatgatagggaca--atatcatatgc-- 83 *F |||||||||||||||||| |||||||||||||||||||||||||| ||||||||||| *F Sbjct: 294 gataaaattctttaaattatttatattaattatatgatagggacacaatatcatatgctg 235 *F *F *F Query: 84 -gtcactaaattgatgacgagctgtttggcaaccatttattttatatcga---atcaagc 139 *F ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| *F Sbjct: 234 cgtcactaaattgatgacgagctgtttggcaaccatttattttatatcgaatcatcaagc 175 *F *F *F Query: 140 aaaggataagctt 152 *F ||||||||||||| *F Sbjct: 174 aaaggataagctt 162 *F \# *F \*g M26817 18S by BLAST: *F *F >EM_INV:DMRG M21017.1 D.melanogaster 18S, 5.8S 2S and 28S rRNA genes, complete, *F and 18S rRNA gene, 5' end, clone pDm238. *F Length = 12026 *F *F Score = 119 bits (60), Expect = 2e-25 *F Identities = 69/72 (95%) *F Strand = Plus / Plus *F *F *F Query: 3 gtatgtaagtgtattactggtggagttcttatatgtgattaaacacttgtattttttcat 62 *F ||||||||| ||||||| ||||||||||||||||||||||||| |||||||||||||||| *F Sbjct: 701 gtatgtaagcgtattaccggtggagttcttatatgtgattaaatacttgtattttttcat 760 *F *F *F Query: 63 atgttcctccta 74 *F |||||||||||| *F Sbjct: 761 atgttcctccta 772 *F \# *F \*g AI124285 18S by BLAST: *F *F >EM_INV:DMRG M21017.1 D.melanogaster 18S, 5.8S 2S and 28S rRNA genes, complete, *F and 18S rRNA gene, 5' end, clone pDm238. *F Length = 12026 *F *F Score = 1304 bits (658), Expect = 0.0 *F Identities = 720/733 (98%), Gaps = 6/733 (0%) *F Strand = Plus / Plus *F *F *F Query: 2 attaccggtggagttcttatatgtgattaaatacttgtattttttcatatgttcctccta 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 713 attaccggtggagttcttatatgtgattaaatacttgtattttttcatatgttcctccta 772 *F *F *F Query: 62 tttaaaaacctgcattagtgctcttaaacgagtgttattgtgggccggtactattacttt 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 773 tttaaaaacctgcattagtgctcttaaacgagtgttattgtgggccggtactattacttt 832 *F *F *F Query: 122 gaacaaattagagtgcttaaagcaggcttcaaatgcctgaatattctgtgcatgggataa 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 833 gaacaaattagagtgcttaaagcaggcttcaaatgcctgaatattctgtgcatgggataa 892 *F *F *F Query: 182 tgaaataagacctctgttctgctttcattggttttcagatcaagaggtaatgattaatag 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 893 tgaaataagacctctgttctgctttcattggttttcagatcaagaggtaatgattaatag 952 *F *F *F Query: 242 aagcagtttgggggcattagtattacgacgcgagaggtgaaattcttggaccgtcgtaag 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 953 aagcagtttgggggcattagtattacgacgcgagaggtgaaattcttggaccgtcgtaag 1012 *F *F *F Query: 302 actaacttaagcgaaagcatttgccaaagatgttttcattaatcaagaacgaaagttaga 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1013 actaacttaagcgaaagcatttgccaaagatgttttcattaatcaagaacgaaagttaga 1072 *F *F *F Query: 362 ggttcgaaggcgatcagataccgccctagttctaaccataaacgatgccagctagcaatt 421 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1073 ggttcgaaggcgatcagataccgccctagttctaaccataaacgatgccagctagcaatt 1132 *F *F *F Query: 422 gggtgtagctacttttatggctctctcagtcgcttcccgggaaaccaaagc-ttttgggc 480 *F ||||||||||||||||||||||||||||||||||| ||||||||||||||| |||||||| *F Sbjct: 1133 gggtgtagctacttttatggctctctcagtcgctt-ccgggaaaccaaagctttttgggc 1191 *F *F Query: 481 tccgggggaagtatggttgcaaagctgaaacttaaaggaattgacggaagggcaccacca 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1192 tccgggggaagtatggttgcaaagctgaaacttaaaggaattgacggaagggcaccacca 1251 *F *F *F Query: 541 ggagtggagcctgcggcttaatttgactcaacacgggaaaacttaccaagtccgaacata 600 *F |||||||||||||||||||||||||||||||||||||||||||||||| || |||||||| *F Sbjct: 1252 ggagtggagcctgcggcttaatttgactcaacacgggaaaacttaccaggt-cgaacata 1310 *F *F *F Query: 601 agtgtgtaagacagattgatagctccttctcgaaatctaaggggtggtggtgcatggccg 660 *F ||||||||||||||||||||||||| |||||| ||||| | ||||||||||||||||||| *F Sbjct: 1311 agtgtgtaagacagattgatagctctttctcg-aatct-atgggtggtggtgcatggccg 1368 *F *F *F Query: 661 ttc-taaatcgtggagtgatttgtccggttaantccgataacgaacgagactcaaatata 719 *F ||| || ||||||||||||||||| |||||| ||||||||||||||||||||||||||| *F Sbjct: 1369 ttcttagttcgtggagtgatttgtctggttaattccgataacgaacgagactcaaatata 1428 *F *F *F Query: 720 ttaaatagatatc 732 *F ||||||||||||| *F Sbjct: 1429 ttaaatagatatc 1441 *F # *U FBrf0155309 *a Adachi-Yamada *b T. *c K. *d Vaccaro *t 2003.2.6 *T personal communication to FlyBase *u *F To: moconnor@mail.med.umn.edu *F Subject: FlyBase Query (cy1739) *F From: Chihiro Yamada *F Date: Thu, 9 Jan 2003 18:39:27 \+0000 *F Dear Dr O'Connor, *F I am currently curating your paper for FlyBase: *F Adachi-Yamada and O'Connor, 2002, Dev. Biol. 251(1): 74--90 *F I have some questions I was hoping you could answer for me. *F .. *F UAS-sal *F \------- *F salmScer\UAS.cdCa *F de Celis et al., 1996, Nature 381(6581): 421--424 *F Lunde et al., 1998, Development 125(21): 4145--4154 *F de Celis et al., 1999, Development 126(12): 2653--2662 *F Expression of a BamHI-KpnI salm cDNA fragment containing the full open *F reading frame is driven by Scer\UAS Scer\GAL4 binding sites. *F salmScer\UAS.cKa *F Bradley and Andrew, 2001, Development 128(15): 3001--3015 *F Chen et al., 1998, Development 125(24): 4959--4968 *F Elstob et al., 2001, Development 128(5): 723--732 *F Kuhnlein and Schuh, 1996, Development 122(7): 2215--2223 *F Expression of salm fragment from nucleotide 370 to nucleotide 5555 of *F the composite cDNA sequence (Kuhnlein et al., 1994, EMBO J. 13(1): *F 168--179) is governed by Scer\UAS regulatory sequences. *F .. *F Date: Sat, 18 Jan 2003 14:19:49 \+0900 *F To: Chihiro Yamada *F From: Takashi Adachi-Yamada *F Subject: Re: Reply to query about our paper *F Hi Chihiro, *F Mike O'Connor told me that the UAS-sal we used was derived from Sean Carroll *F in Univ. Wisconsin. It was also labelled with '43A'. The insertion is on *F the 3rd chromosome. These are all we know about the UAS-sal line. *F Best regards, *F Takashi *F .. *F Hi Chihiro, *F Sorry for the delay of my response. As I told you, the UAS-sal43A is not *F our original. So I would like you to inquire to Sean Carroll *F (sbcarrol@facstaff.wisc.edu) about recording it as a new allele in the *F FlyBase. *F Thanks in advance, *F Takashi *F .. *F [FlyBase curator comment. Email sent to Sean Carroll. Kathy Vaccaro *F replied on his behalf] *F From kvaccaro@facstaff.wisc.edu Mon Jan 27 14:49:36 2003 *F Date: Mon, 27 Jan 2003 08:49:33 \-0600 *F From: Kathy Vaccaro *F Subject: uas-sal line *F Dear Chihiro Yamada, *F I checked our fly stock list and the line w;Cyo/+;UAS-sal 43A/TM2 has *F listed for a source as Rosa Barrio. *F .. *F That is all the information I have. Hope it helps. # *U FBrf0155312 *a Fujioka *b M. *c R. *d Bodmer *t 2003.2.6 *T personal communication to FlyBase *u *F Date: Thu, 6 Feb 2003 16:43:50 \-0500 (EST) *F From: Rolf Bodmer *F Sender: *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (cy1756) *F ... *F Question 2: *F EVEG92 is the one used to make the 86T, delta ES, and delta NS. The 86T *F construct has a \+8.6kb endpoint, and both delta ES and NS have a \+9.2kb *F endpoint. A wild type construct that has either endpoint gives the same *F expression pattern. *F thanks, rolf *F On Wed, 5 Feb 2003, Chihiro Yamada wrote: *F > Dear Dr Bodmer, *F > *F > I'm currently curating your paper for FlyBase: *F > *F > Han et al. 2002, Dev. Biol. 252(2) 225--240. *F > *F ... *F > tkv1A&Dgr;GS.Scer\UAS.T:Ivir\HA1 *F > P{UAS-tkv.1A&Dgr;GS} *F > A dominant negative form of the tkv1 isoform, in which the GS *F > box is deleted, tagged with HA1, is expressed under the control *F > of Scer\UAS regulatory sequences. *F > *F > Could you tell me which one of these you used in your paper? *F > *F > *F > *F > *F > eve rescue construct *F > ==================== *F > In Fig 7 you describe 2 eve transgene constructs, that have been made by *F > deleting portions of the eve mesoderm enhancer. These were made from a *F > eve rescue construct for which you cite: *F > *F > Fujioka et al., 1999, Development 126(11) *F > *F > In this paper, 2 constructs were made that meet the description. *F > *F > P{E+L-eve.9.2.G} called EVEG92 in the Fujioka paper *F > P{E+L-eve.9.2} called EVE92 *F > *F > The only difference is the presence of w+mCG (a w+mC allele that *F > has been modified by the addition of a gl activator sequence) in *F > EVEG92. *F > *F > Can you tell me which one of these was used to make 86T insertion, and *F > whether this was the same construct that was used as the basis for the *F > P[eve+,eme&Dgr;ES] and P[eve+,eme&Dgr;NS] constructs? *F > *F > Best wishes, *F > *F > Chihiro *F > *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F > Department of Genetics, *F > University of Cambridge, email: c.yamadagen.cam.ac.uk *F > Downing Street, Tel : 01223-333963 *F > Cambridge, CB2 3EH, FAX : 01223-333992 *F > United Kingdom. Memes don't exist. Spread the Word. *F > \---------------------------------------------------------------------- *F Dear Chihiro, *F in the pers. communication, please, include Miki Fujioka, who made the T86 *F construct and related transgenic insertion lines. J48 and J43 are *F insertion lines of the delta ES and delta NS construct, respectively *F (+9.2kb endpoint). In fact, we tested another delta ES insertion line, *F J49, which also abolished mesodermal expression, as J48. *F I hope this clarifies the issue. thanks, rolf *F On Fri, 7 Feb 2003, Chihiro Yamada wrote: *F > Dear Rolf, *F > *F > Thanks for your prompt reply. *F > *F > > Question 2: *F > > EVEG92 is the one used to make the 86T, delta ES, and delta NS. The 86T *F > > construct has a \+8.6kb endpoint, and both delta ES and NS have a \+9.2kb *F > > endpoint. A wild type construct that has either endpoint gives the same *F > > expression pattern. *F Dr. Rolf Bodmer Tel: 734-763-3182 *F Dept. of MCDB, NS3013 Fax: 734-647-0884 *F University of Michigan E-mail: rolf@umich.edu *F 830 N University *F Ann Arbor, MI 48109-1048 *F http://biology.lsa.umich.edu/research/labs/bodmer/index.htm # *U FBrf0155314 *a Badenhorst *b P. *t 2003.2.13 *T personal communication to FlyBase *u *F Date: Wed, 12 Feb 2003 12:06:55 \-0500 *F Subject: Fwd: FlyBase Query (cy1763) (fwd) *F Cc: Carl Wu *F To: cy200@gen.cam.ac.uk *F From: 'Paul Badenhorst' *F Hi Chihiro, *F In answer to your questions... *F Df(3L)XZB970 was reported in Rebay et al, Genetics 154(2), 695-712, *F 2000. It complements trachealess, and fails to complement nurf301 and *F CG7036. *F Df(3L)rH321 is originally from the Berkeley P-element insertion *F collection, known as l(3)rH321. Matt Voas inverse PCRed off the 5' and *F 3' ends of this insertion and realized that they lie about 50kb apart. *F The proximal end is to the right of the nurf301 coding region and the *F distal end is to the right of the CG7036 coding region. He confirmed *F by PCR that this region is deleted rather than having two insertions *F that would confound the inverse PCR results. Genetically, Df(3L)rH321 *F fails to complement nurf301 but complements CG7036. *F Df(3l)3643 is an excision line derived from EP(3)3643. It removes *F nurf301 entirely. I mapped the proximal to within 2 kb of the nurf301 *F ATG. It leaves CG7020 intact. Distally, the deficiency removes at *F least to CG17142, however this breakpoint has not been precisely *F mapped. *F I hope this helps. *F Sincerely, *F Paul. *F Begin forwarded message: *F > From: Carl Wu *F > Date: Tue Feb 11, 2003 05:34:25 US/Eastern *F > To: Paul Badenhorst *F > Subject: FlyBase Query (cy1763) (fwd) *F > *F > *F > please reply *F > Carl Wu, Ph.D. *F > Chief, Laboratory of Molecular Cell Biology *F > National Cancer Institute *F > NIH Bg. 37, Rm 6068 *F > Bethesda, MD 20892-4255 *F > *F > Tel. 301-496-3029 *F > Fax. 301-435-3697 *F > *F > *F > \---------- Forwarded message \---------- *F > Date: Tue, 11 Feb 2003 18:21:49 \+0000 *F > From: Chihiro Yamada *F > To: carlwu@helix.nih.gov *F > Cc: cy200@gen.cam.ac.uk *F > Subject: FlyBase Query (cy1763) *F > *F > Dear Dr Wu, *F > *F > I am currently curating your paper for FlyBase: *F > *F > Badenhorst et al., Genes Dev. 2002 16(24), 3186--3198 *F > *F > I have a few question I was hoping you could answer for me. *F > *F > Df(3L)XZB970 *F > ============ *F > We don't have a Deficiency of this name in our records. Has it been *F > published before? If so under what name? *F > *F > *F > Df(3L)rH321 *F > =========== *F > We don't have a Deficiency of this name in our records. Has it been *F > published before? If so under what name? What relationship does it *F > have to the insertion P{PZ}l(3)06240rH321, Was this insertion used to *F > make this line by imprecise excision? *F > *F > Df(3L)3643 *F > ========== *F > You make this Deficiency from P{EP}EP3643. Do you any information *F > about the breakpoints in this Df? Are either of them within the *F > nurf301 gene? *F > *F > Any new information you give us will be curated as a personal *F > communication form you to FlyBase, if thats fine with you. *F > *F > Best wishes, *F > *F > Chihiro *F > *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F > Department of Genetics, *F > University of Cambridge, email: c.yamada@gen.cam.ac.uk *F > Downing Street, Tel : 01223-333963 *F > Cambridge, CB2 3EH, FAX : 01223-333992 *F > United Kingdom. Memes don't exist. Spread the Word. *F > \---------------------------------------------------------------------- *F > *F > *F Paul Badenhorst *F NCI Laboratory of Molecular Cell Biology *F Bldg 37 Rm 6066 *F 37 Convent Drive *F Bethesda MD 20892-4255 *F USA # *U FBrf0155315 *a Labrador *b M. *t 2003.3.14 *T personal communication to FlyBase *u *F To: corces@jhu.edu *F From: Chihiro Yamada *F Date: Tue, 11 Feb 2003 18:03:28 \+0000 *F Dear Dr Corces, *F I am currently curating your paper for FlyBase: *F Labrador and Corces, Genes Dev. 2003 17(1), 43--48 *F I have a quick question I was hoping you could answer for me. *F At the beginning of your materials and methods section, *F you cite 3 Bloomingtons stocks. 1521, 1522 and 4775. The first two *F include the transgene construct, *F P{UAS-GFP.S65T} *F 4775 includes: *F P{UAS-GFP.nls} *F Which also has a nls signal. *F You describe a couple of new insertions of a UAS-GFP construct. Can *F you tell me which transgene construct you used to make them? *F pUAST-Hsp70:GFP4.1.3 *F pUAST-Hsp70:GFP 22.21 *F Best wishes, *F Chihiro *F \---------------------------------------------------------------------- *F Date: Wed, 12 Feb 2003 14:34:44 \-0500 *F From: Mariano Labrador San Jose *F To: cy200@gen.cam.ac.uk *F Dear Dr Yamada, *F Dr Corces just gave me your e-mail message asking for the origin of *F transgenes pUAST-Hsp70:GFP4.1.3 and pUAST-Hsp70:GFP22.21. I just realized *F that there are several typos in the paper. These lines are in fact *F pUASp-P/T:GFP lines, not pUAST-Hsp70:GFP lines. Looking at the material and *F methods of the paper I also noticed that there is another mistake concerning *F the line pUASp-P/T:GFP1.5 line. *F The pUASp-P/T:GFP lines were generated in our lab using a GFP sequence *F cloned in the cloning site of the pUASp vector. The name of the three lines *F is corrected bellow. They correspond to insertions in different chromosomes *F of the same P element. *F I apologize for the mistakes. Hopefully this e-mail will help you for the *F curation of the manuscript. *F pUAST-Hsp70:GFP1.5 it should say pUASp-P/T:GFP1.5 *F pUAST-Hsp70:GFP4.1.3 it should say pUASp-P/T:GFP4.1.3 *F pUAST-Hsp70:GFP22.21 it should say pUASp-P/T:GFP22.21 *F Mariano Labrador, Ph.D. *F Department of Biology *F The Johns Hopkins University *F Baltimore, MD # *U FBrf0155317 *a Rushton *b E. *t 2002.3.12 *T personal communication to FlyBase *u FlyBase error report for CG15864 on Tue Mar 12 15:25:04 2002. *F Date: Tue, 12 Mar 2002 15:25:04 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: rushton@biology.utah.edu *F Subject: FlyBase error report for CG15864 on Tue Mar 12 15:25:04 2002 *F Error report from Emma Rushton (rushton@biology.utah.edu) *F Gene or accession: CG15864 *F Release: 2 *F cDNA or EST error *F Comments: The annotation CG18749 corresponds to a dicistronic transcript. Both *F ORFs were predicted in Release 1 but only the 3' most, represented by *F CG18749, is predicted in Release 2. *F I enclose the sequence with the beginnings and ends of the two open *F reading frames marked with \**. This sequence was obtained by completely *F sequencing the EST clone GH05783. There are multiple stop codons in all three *F frames, between the two ORFs. *F ATAGACGTAC ACCCACAACC CCAATTCCAA CCGGCAAGTT CCGGAACGTA *F GTCTCTTCTT TAAAAGTCTA TAGAGGGAAG TGGCCAATCC G**ATG**GACAAG *F CGCGTAACAG TCCTGCTGCC CAATGGTCGG CGGCAGAATG TGAACGTGAC *F GGCGAACATG ACGCTGTTGG AGATTCTGGA GACGGCCTGC TTCAAGCACG *F GCTACGATGC GGAGGAGCAC TGCCTCAAGT TTCACAACAA GGTGGTCGGA *F TTGACGCAAC AGTTCCGTTT TAGTGGCCTG CCTAACAACT GTGTCCTGGA *F GATGGAGCAA ACAGAAAAGC GGCGCACACT GAGTAACGTT CTTGTCTGCG *F TCCAATTGAA CGACGGTTCC CGCCAGCAAG GGGACTTCTC GCCCAACGAC *F ACCATATGGC TGGTGGTCGA GAAGCTTTGC GAAACACATG TAAAGGCTTA *F CGAAAGCCCC GTCATCATCT ACATGCGCAG CGAGGTCATA GGACATGAGC *F AGATGCGACA GACCACTCTT AAGTCCCTAG GAATCCTCGA GGGTCGTGCC *F ATGATGCGTC TTATCGACAA AAAACCCGAG GACCTTAAGA CCCAAGCAAA *F TGTGTATAAA GCTCCCGCTG TCAAGCCTCG GGTGGACAAT GACGACCAGC *F CCAGTACCTC CCGCTCTGCA ATGGCAGCTG GTGGCAGCGG AGGAGGAGGT *F GGATTTGCTC TTACCAGCAA CATGATCAAG AATTTGAAAC GCACGGCTCC *F TGAAGAAAAG GCCAGCGGTA GCGAACCAGC CAAGCCCTTA GGGGAATCCA *F ATCAAGAGCA GCAGCCTCAG CCAGAACCAC CTAAATATGA CTGGGGCAAT *F GGTTCCGGAT ACTTAATGAA CCACCCTCCC GAGCAGAAGC AGGCAGAAAA *F CGAAGTCGAA GAGAATCCAG GCAGGGCTCC ACCAGTGGTG AAAATAATTG *F GGCCACGCCA GGCTGTACTC TTTTCCTTGG ACGAATCCAA GAAAAATGCG *F AATGACCTGC CGGACTCATT TTTCGACTTG ACCGTCAACG ATTTGAAGAT *F GGTACTCCGT GATTTAAAGC GTACGTCGAG CGGAGATGAC GACGCTCCCT *F TGCTGACAGC AAAGCTAAGG GAATTGGAGC GCCAAAAGGC CATGCTGGCT *F AAGCTTAACC AGTACAAGGA CTGTGTGCTG CGCATTCAGT TTCCCGATCG *F CTTCGTGCTC CAGGGCATGT TTAAGCCGCA CGAGCCCCTT TCCAAGGTGG *F AGGATTTTGT TCGGGAGTTT TTGGTTCAGC CAGGTGAGCA ATTCCATCTC *F TTCACCATTC CACCGAANAA GGTGCTGCCC TCCGGTGAGA CGCTCCTGGA *F GCTGAATTTT GTTCCAAATG CCATCGTGCA TTTTGGCTTT ATCAAGGACT *F CGCTCAATGC GGTGAGCAAT CGATTCGTGA AGGAGGAGTA CGTGGATCAG *F TTGACCTCCG AGGAGGGAGC GCACTACGCC AGTGCAAAAG TACCGCCTTA *F CCCGTGCAAC GGCCTCAGGG TC**TAG**TTAAT AGATACATAC ATACAGTCTG *F TGTGATATTA AATGAATCCG CTTTATTTCT ATATATTTTT TTATTTGACA *F CATTTTATAT AAAATGAGAA TTGTCGTTAT ATTCTTTATC AAATGAAACT *F GGAAAACTGA CAGTAATACG CTGAGCCAGG CTTTAACATG TTCTTTTTTT *F TTTTAAACAT GTGTTAGACA AAAATCCACT ATCGCATAAC AATCATGAGC *F TGGCAGTGAT AAAGCATTGC TAATTTAATT AGACCGCGAC AAATTTCTTA *F TTGTCAATGT TATCATTACC ATGTTGTAAA TTCATTTGCC AATACATCTC *F AAAAGTGAA \**ATG**TACATCTT AAAAAAATTA ACGTTTATTG GCTTTCTGAG *F TGCTTGTTTT ATTAACTGCC AAGGATTTGT CAATTCGAAC CCAAAGAAAT *F CTTATGCTGC CTCAGCAATG GAACTGATGA AACTCCTAGA GGTGGAGGAT *F GAACTGGTAG ACAATTTAAA GGGCTATGTG AAAACACTGA AAATGAAATT *F CAATTTAATN GAAAGATCCC TAATAGATAT GAGTAGAGAG AACATGGAAA *F TGAAGAGTGA TTATGAATCG TACTTGGGAA ATCCGTTGAA CAGCTTTCGA *F CTAATACATC GCCTACACAC GAGTTGGAGA AAATGGTATC AATATGCCAT *F TAAGGTCGAG AATAATGCTT TAGGGCACAT TGAAAATGCC CGACTGATGA *F GAAAGATGCT ACCAACCTCG TCGGATTTGC AGCAGGCGTG TCGCGGAATC *F CATGACTTGA TGTACTTTTA CGATTTGAAG CCAGAAGAAC TGGCAGCTGG *F TAACTTAGCA GGGTACTCGC AGCCAGGAAC AGGACTGACA GCTTACGATT *F GTTTAGCCCT TGGCGAGTTT GGCGTCCAAA ATCAAAAGGA TGATCTTGCC *F GAGGCTTGGT ATAACCTTTC TTTGACTCGT TTTGATAATA TAATCGAAAA *F ATACCAAGTG CACAAGGCAT GGGCACTTCT CCTGGCGAAG AATAAGCAAC *F TTACTGAAGC CTTTCAACAT TTCGAGAACA AGCCTGAGGG AATAGTCGCC *F AGTAATGAAG TTATACACTT TGAGGGTGTG TTGGCGACTA CGCAAAACTG *F CACTGCTGTG GTCCAAAAAC CAAGTAAAAA ACTCCACTGT CGCTACAACA *F CCTCCACGAC TCCCTTCACG CGGATTGCCC CTCTAAAAAT GGAGGAGCTG *F GGCTTAGATC CGTACATGGT GGTCTTTCAC GATGTGATTT ACGACACCGA *F AATTGATGGG ATGCTAAACT CGAGTGANTT TGGATTATCC GAGTCTGTTT *F CTGGTCTGAA GTCTGAGGTG AGAACGTCCA AGGACTCACA TATAGTAGAT *F GCTAAAACAC TCAATGAGCG CGTAACCGAT ATGACCGGAC TCAGTATGGA *F GATGAGCGAT CCATTTTCGC TGATTAATTA CGGCTTAGGT GGGCACTTCA *F TATTGCACCA TGACTTTCAT GAATATACGA ATACGACCAG GCTTAAGCAA *F GGGGATTGTA TAGCTACTGT TTTGTTTTAC CTGAGAGAAG TTGACTCGGG *F TGGCGCCACA GTTTTCCCGA TGCTTAATAT TACGGTTATG CCCAAGAAGG *F GATCAGCGGT TTTTTGGTAC AATTTTCACA AATCAGGAGC TGTAAATTCG *F AAAACTTTGC ACACAGCTTG TCCAGTGATA AGCGGTTCGA AATACGTGCT *F CACGAAGTGG ATAAACGAAC TGCCGCAAAT GTTTGTCACA CCATGTATGA *F AAGAATTGAA CCTACGCCCA CCCCAAAAAG AA**TAA**TTTTG TAATTTGTAA *F TGGAATTAAA TTTAAATTAA ATTTGTTTTC CCTTTTTTGG ATG # *U FBrf0155319 *a Peifer *b M. *t 2002.6.27 *T personal communication to FlyBase *u FlyBase error report for CG14768 on Thu Jun 27 10:51:00 2002. *F Date: Thu, 27 Jun 2002 10:51:00 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG14768 on Thu Jun 27 10:51:00 2002 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG14768 *F Release: 3 *F Missed gene *F Comments: It looks like this may be a retrotransposon remnant. If you blast *F search with the predicted mRNA translated in three frames, you hit a gag=pol *F polyprotein precursor matching the cysteines and the active site *F >gi|20129709|ref|NP_610160.1| LocusLink info (NM_136316) CG14768 gene *F product Drosophila melanogaster *F gi|7302250|gb|AAF57343.1| LocusLink info (AE003787) CG14768 gene product *F Drosophila melanogaster *F Length = 111 *F Score = 227 bits (579), Expect = 2e-59 *F Identities = 111/111 (100%), Positives = 111/111 (100%) *F Frame = \+1 *F Query: 1 MAPRPRAISTAQTASPIGTQEETSAAKRDARSVEGITTPYSTCMRNSVQIRQWHRCHLRH 180 *F MAPRPRAISTAQTASPIGTQEETSAAKRDARSVEGITTPYSTCMRNSVQIRQWHRCHLRH *F Sbjct: 1 MAPRPRAISTAQTASPIGTQEETSAAKRDARSVEGITTPYSTCMRNSVQIRQWHRCHLRH 60 *F Query: 181 AKRVSTLHLVRCPSPGLRPEPQSPTIARPQKKSVHILPTAIVVLDTGSRTF 333 *F AKRVSTLHLVRCPSPGLRPEPQSPTIARPQKKSVHILPTAIVVLDTGSRTF *F Sbjct: 61 AKRVSTLHLVRCPSPGLRPEPQSPTIARPQKKSVHILPTAIVVLDTGSRTF 111 *F >gi|21298156|gb|EAA10301.1| (AAAB01008944) ebiP5034 [Anopheles gambiae str. *F PEST] *F Length = 691 *F Score = 42.4 bits (98), Expect = 0.001 *F Identities = 21/47 (44%), Positives = 25/47 (52%) *F Frame = \+3 *F Query: 21 HKYCPNCLAHRHSGGDFRSKEGCKKCGGNNHTLLHMHEELRSDPTVA 161 *F HK C NCL H S+ GC+ CG \+HTLLH RSD \+A *F Sbjct: 237 HKLCWNCLQGSHFVTSCTSRYGCQTCGKRHHTLLHAE---RSDSVIA 280 *F >gi|21302777|gb|EAA14922.1| (AAAB01008984) agCP4897 [Anopheles gambiae str. *F PEST] *F Length = 957 *F Score = 40.4 bits (93), Expect = 0.004 *F Identities = 19/50 (38%), Positives = 24/50 (48%) *F Frame = \+3 *F Query: 30 CPNCLAHRHSGGDFRSKEGCKKCGGNNHTLLHMHEELRSDPTVASMPPPT 179 *F C NCL H+ \+SK C+KC \+HTLLH \+ T SM T *F Sbjct: 263 CENCLGKNHAANRCKSKYTCRKCKQRHHTLLHKEPVHPTTSTTISMQNTT 312 *F >gi|21214752|emb|CAD32253.1| (AJ487856) gag-pol polyprotein precursor; *F hypothetical protein *F Drosophila melanogaster *F Length = 1830 *F Score = 38.9 bits (89), Expect = 0.012 *F Identities = 20/52 (38%), Positives = 25/52 (47%) *F Frame = \+3 *F Query: 30 CPNCLAHRHSGGDFRSKEGCKKCGGNNHTLLHMHEELRSDPTVASMPPPTRK 185 *F C NC A H D S C C G \+HTLLH S+ \+S PPT++ *F Sbjct: 464 CLNCFARGHQLRDCTSMHSCFTCKGRHHTLLH-RSPPNSENASSSTSPPTQQ 514 # *U FBrf0155320 *a Peifer *b M. *t 2002.7.1 *T personal communication to FlyBase *u FlyBase error report for CG30442 on Mon Jul 1 06:52:16 2002. *F Date: Mon, 1 Jul 2002 06:52:16 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG30442 on Mon Jul 1 06:52:16 2002 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG30442 *F Release: 3 *F Missed gene *F Comments: CG30442 is a probable retrotransposon remnant. The predicted *F protein matches several retroviral env proteins *F >gi|19527951|gb|AAL90090.1| (AY089352) AT17684p Drosophila melanogaster *F gi|21626854|gb|AAM68371.1| (AE003787) CG30442-PB Drosophila melanogaster *F Length = 126 *F Score = 234 bits (596), Expect = 2e-61 *F Identities = 126/126 (100%), Positives = 126/126 (100%) *F Query: 1 MESSSWLTWLTLEKCDNLWTKSLKMPLPCRKGGLRSFLSTHLKGATECVNRIASAPVRKP 60 *F MESSSWLTWLTLEKCDNLWTKSLKMPLPCRKGGLRSFLSTHLKGATECVNRIASAPVRKP *F Sbjct: 1 MESSSWLTWLTLEKCDNLWTKSLKMPLPCRKGGLRSFLSTHLKGATECVNRIASAPVRKP 60 *F Query: 61 CSINWLGSAWKWIAGNPDAADWNTILATQKVPLKNSDQQLRINSRLFDATHESITNKRGH 120 *F CSINWLGSAWKWIAGNPDAADWNTILATQKVPLKNSDQQLRINSRLFDATHESITNKRGH *F Sbjct: 61 CSINWLGSAWKWIAGNPDAADWNTILATQKVPLKNSDQQLRINSRLFDATHESITNKRGH 120 *F Query: 121 RNSNCH 126 *F RNSNCH *F Sbjct: 121 RNSNCH 126 *F >gi|21297620|gb|EAA09765.1| (AAAB01008907) agCP7298 [Anopheles gambiae str. *F PEST] *F Length = 475 *F Score = 45.1 bits (105), Expect = 2e-04 *F Identities = 29/82 (35%), Positives = 44/82 (53%), Gaps = 3/82 (3%) *F Query: 31 KGGLRSFLSTHLKGATECVNRIASAPVRKPCSINWLGSAWKWIAGNPDAADWNTILATQK 90 *F K L+S \++ L+ \+++I \+R N \+G+AWKWIAG+PDA D I T \+ *F Sbjct: 47 KSPLQSLINLKLEKPNATISKIRPRRLRTK-RWNSIGTAWKWIAGSPDAEDLTIINTTLE 105 *F Query: 91 VPLKNSDQQLRIN--SRLFDAT 110 *F \+N++Q L N SR F T *F Sbjct: 106 FAHQNNEQLLINNGLSRRFQET 127 *F >gi|15384852|emb|CAC59744.1| (AJ308094) putative retrovirus-like env *F glycoprotein [Drosophila *F virilis] *F Length = 488 *F Score = 42.7 bits (99), Expect = 8e-04 *F Identities = 22/65 (33%), Positives = 42/65 (63%), Gaps = 7/65 (10%) *F Query: 62 SINWLGSAWKWIAGNPDAADWNTILATQKVPLKNSDQQLRINS-------RLFDATHESI 114 *F S+++LG+A K \+AG PDA+D+ I T+ \++ \+ \+Q++INS RL D \++ I *F Sbjct: 102 SLDFLGTALKVVAGTPDASDFLRIRVTETQLVEANSKQIQINSETQKQINRLTDTINKII 161 *F Query: 115 TNKRG 119 *F \++++G *F Sbjct: 162 SSRKG 166 *F >gi|2133742|pir||S64735 retrovirus-related env protein homolog \- fruit fly *F (Drosophila *F subobscura) retrotransposon gypsy *F gi|1237206|emb|CAA51085.1| (X72390) ORF3 Drosophila subobscura *F gi|1588534|prf||2208454C ORF 3 Drosophila subobscura *F Length = 384 *F Score = 40.8 bits (94), Expect = 0.003 *F Identities = 21/65 (32%), Positives = 42/65 (64%), Gaps = 7/65 (10%) *F Query: 62 SINWLGSAWKWIAGNPDAADWNTILATQKVPLKNSDQQLRINS-------RLFDATHESI 114 *F S+++LG+A K \+AG PDA+D+ \+ T+ \++++ \+Q+ INS RL D \++ I *F Sbjct: 84 SLDFLGTALKVVAGTPDASDFLKVRITEAQLVESNSKQIIINSETQKQINRLTDTINKII 143 *F Query: 115 TNKRG 119 *F \++++G *F Sbjct: 144 SSRKG 148 # *U FBrf0155321 *a Peifer *b M. *t 2002.7.1 *T personal communication to FlyBase *u FlyBase error report for CG30441 on Mon Jul 1 07:00:48 2002. *F From FlyBase-error@ogre.lbl.gov Mon Jul 01 15:09:45 2002 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Mon, 1 Jul 2002 15:09:45 \+0100 *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 1 Jul 2002 07:00:48 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG30441 on Mon Jul 1 07:00:48 2002 *F Content-Length: 415 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG30441 *F Release: 3 *F Missed gene *F Comments: Blast searching suggests this may be the ortholog of intraflagellar *F transport protein 20, AAL07274 or AAL99902.1 # *U FBrf0155322 *a Peifer *b M. *t 2002.7.1 *T personal communication to FlyBase *u *F Date: Mon, 1 Jul 2002 09:18:44 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG30439 on Mon Jul 1 09:18:44 2002 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG30439 *F Release: 3 *F Missed gene *F Comments: While the predicted ORF has no matches in blast, if one searches the *F predicted mRNA in all six frames, the \-3 frame has a significant match to ORF *F 1 of jockey *F gi|120799|sp|P21330|GAGJ_DROME Nucleic-acid-binding protein... 56 5e-07 *F >gi|120799|sp|P21330|GAGJ_DROME Nucleic-acid-binding protein (Mobile element *F jockey) (ORF1) *F Length = 568 *F Score = 55.8 bits (133), Expect = 5e-07 *F Identities = 29/94 (30%), Positives = 43/94 (44%), Gaps = 16/94 (17%) *F Frame = \-3 *F Query: 235 DTYRQVARHFLKKGSLFHHHQLPEDRPYRIVLRSIHHEVSSKDIIACLQNEGHSLVRVYT 56 *F D \+R \+ K F HHQL E++PYR+VL+ IH V S I \+ G+ \++ \+Y *F Sbjct: 264 DAFRTAVKELNKLNCQFWHHQLKEEKPYRVVLKGIHANVPSSQIEQAFSDHGYEVLNIYC 323 *F Query: 55 PR----------------NKSISLPLNMIYIDLK 2 *F PR K+ N+ YI+LK *F Sbjct: 324 PRKSDWKNIQVNEDDNEATKNFKTRQNLFYINLK 357 # *U FBrf0155323 *a Peifer *b M. *t 2002.7.8 *T personal communication to FlyBase *u FlyBase error report for CG2682 on Mon Jul 8 09:44:14 2002. *F Date: Mon, 8 Jul 2002 09:44:14 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG2682 on Mon Jul 8 09:44:14 2002 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG2682 *F Release: 3 *F Gene annotation error *F Gene CG2682 has incorrect exon/intron structure or translation start site. *F Comments: The B transcript (the long one with alternative exons far 5' to the *F coding sequence may be bogus. The first few hundred basepairs of this *F transcript match in reverse complement an S element (as does the second best *F est match, HL02569) *F >gb|U33463|S-element DM33463 1736bp Derived from U33463 (g1006788) (Rel. 47, *F Last updated, Version 5). *F Length = 1736 *F Minus Strand HSPs: *F Score = 707 (112.1 bits), Expect = 9.0e-29, P = 9.0e-29 *F Identities = 193/235 (82%), Positives = 193/235 (82%), Strand = Minus / Plus *F Query: 420 TGTCAAGAAACTCT--ACACA-TTTTGTTTTCATACTTTGTTTTGCTCATTTTCTTAGAA 364 *F || ||||||||||| ||||| ||||| ||| ||||||||||||||| ||||||||||| *F Sbjct: 214 TGCCAAGAAACTCTTTACACAGTTTTGGGTTCCTACTTTGTTTTGCTCTTTTTCTTAGAA 273 *F Query: 363 CGAA-CACAATTTTTCCGTTATTTTTGGATT-TGCATTGCCTTTTACAATGCTTCTATTG 306 *F || | || ||||||||||||||||| || |||||| | |||||||| |||||||||| *F Sbjct: 274 ACAATCTCA-TTTTTCCGTTATTTTTGTCTTATGCATTCCTTTTTACAACGCTTCTATTG 332 *F Query: 305 AGATTTTTTTGACTTTGCTTGCGAACTTTTGCTGATCAAATGTGCTTAAAGCAAATTATT 246 *F ||||||| |||||||||| ||| ||||| ||||| || ||||||||||| ||||||| *F Sbjct: 333 CAATTTTTTC-ACTTTGCTTGTGAAATTTTGTTGATCTAACGTGCTTAAAGCGAATTATT 391 *F Query: 245 AAATTTAATATTTAATAAAAATGCCTGGAAAGAGATTAACTTTTGAACTTACCCA 191 *F ||||||||| ||| |||||||||||||||| |||||||| | ||||| *F Sbjct: 392 AAATTTAATG------AAA--TGCCTGGAAAGAGATTGGCTTTTGAAGTGACCCA 438 *F while the best est match to this region of the gene, GM22420, matches a *F trasnsib element *F >transib1 TRANSIB1 2167bp *F Length = 2167 *F Minus Strand HSPs: *F Score = 234 (41.2 bits), Expect = 7.9e-05, P = 7.9e-05 *F Identities = 160/261 (61%), Positives = 160/261 (61%), Strand = Minus / Plus *F Query: 311 GATTTT-GTGGTACAGC-AACGCCAG--ACGAAGGTATATGATGGTGATGCTGATGCGTT 256 *F ||||| | | | ||| ||||| | ||| || ||||| | || | ||| || *F Sbjct: 1710 GATTTGCGCAGCAAAGCCAACGCATTTAATGAATGTGAATGATTTTAATTCAGATATTTT 1769 *F Query: 255 TGATGGTGGGAGTGT-AAACTTTTCACTCCTCGGCGAAAGATCGATCTCCCGGCGCAGAT 197 *F ||||| | | | | ||||| |||| | | | ||| | | | | | ||| ||| *F Sbjct: 1770 TGATGCTAA-A-TCTCAAACTCTTCAGTT-T--G-GAATAAGCCCTTTACATGCGTGGAT 1823 *F Query: 196 GAGCCAATTCTGTATTCTGTATGAATA-TTGTAGTTACGTATGCACAGAAACTAAACATT 138 *F || || || ||| ||| | || | ||| | | || | || || |||| || *F Sbjct: 1824 TCGCTTCTT-TGAATT-TGTTTTAAAACTTGCA-T-ACAGA-GCT--GATCTTAAAACTT 1876 *F Query: 137 AAC-TA-TTTAAAGTCATTTTTTAAATTTATTTTATTATCTTTAC-ACATGAGTTTTACT 81 *F | || |||| | || | | | | ||||||||||| | ||| | | || | | || | *F Sbjct: 1877 GGCATAATTTACA-TCTTAATATGATTTTATTTTATTTTATTTTCTATAT-A-TAATA-T 1932 *F Query: 80 TAAGATAA-ATTTAATTAAAT 61 *F || | || |||||||||||| *F Sbjct: 1933 CAATAAAATATTTAATTAAAT 1953 # *U FBrf0155324 *a Isaac *b R.E. *t 2002.7.9 *T personal communication to FlyBase *u FlyBase error report for CG7105 on Tue Jul 9 09:20:33 2002. *F Date: Tue, 9 Jul 2002 09:20:33 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: r.e.isaac@leeds.ac.uk *F Subject: FlyBase error report for CG7105 on Tue Jul 9 09:20:33 2002 *F Error report from R.E Isaac (r.e.isaac@leeds.ac.uk) *F Gene or accession: CG7105 *F Release: 2 *F Missed gene *F Protein sequence: *F >Proct (CG7105) *F MGVPRSHGTGIGCGSGHRWLLVWMTVLLLVVPPHLVDGRYLPTRSHGDDLDKLRELMLQILELSNEDPQQQQQQQQQQQ *F HPQLRLHNEATGGSSSSSNINNPRVSNGNSNAAWLQKLSAMGALDELGGDGARFGPNYGRY *F Comments: We believe that the start Met has been wrongly predicted. There is *F a much better Kozak Consensus sequence for the translation initiation codon at *F nt 317 of the cDNA. Changing the start Met in this way does not alter the *F predicted signal peptide cleavage site at VDG-RY , but does give a much better *F score. *F We have submitted a paper for publication describing the identification of *F CG7105 as the gene encoding the insect neuropeptide, proctolin. We have given *F the gene name Proct for CG7105. *F Details have been submitted to EMBL (accession number AJ458447) to be released *F later this month. # *U FBrf0155325 *a Posakony *b J. *t 2002.7.11 *T personal communication to FlyBase *u *F Date: Thu, 11 Jul 2002 15:27:19 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: jposakony@ucsd.edu *F Subject: FlyBase error report for CG15064 on Thu Jul 11 15:27:19 2002 *F Error report from Jim Posakony (jposakony@ucsd.edu) *F Gene or accession: CG15064 *F Release: 2 *F Missed gene *F Comments: A new paper from our lab: *F Rebeiz, M., Reeves, N. L., and Posakony, J. W. (2002). SCORE: A computational *F approach to the identification of cis-regulatory modules and target genes in *F whole-genome sequence data. Proc. Natl. Acad. Sci. USA, in press (available *F now in PNAS Early Edition) *F shows that CG15064, to which we have given the name Him, is expressed in adult *F muscle precursor cells in both embryos and imaginal discs, under the direct *F control of Suppressor of Hairless and probably Twist. *F We would also like to note the presence of two putative post-transcriptional *F regulatory elements in the 3' UTR of this gene: *F (1) A 'Bearded (Brd) box' (AGCTTTA) [Lai and Posakony (1997) Development 124: *F 4847-4856] at position 58317-58323 of AE003508.2 *F (2) A 'K box' (TGTGAT) Lai et al. (1998) Development 125: 4077-4088 at *F position 58317-58323 of AE003508.2 *F Thanks! # *U FBrf0155326 *a Whitfield *b E. *t 2002.7.22 *T personal communication to FlyBase *u FlyBase error report for CG11217 on Mon Jul 22 13:21:28 2002. *F Date: Mon, 22 Jul 2002 13:21:28 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG11217 on Mon Jul 22 13:21:28 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG11217 *F Release: 3 *F Gene annotation error *F Gene CG11217 has incorrect exon/intron structure or translation start site. *F Comments: AE003840; AAF59195 annotation is missing an N-terminal exon that *F encodes the initiating Met. This exon is seen in nucleotide entry U56245: *F FT CDS join(complement(2679..2681),complement(2408..2447), *F FT complement(1415..1713),complement(1186..1356)) *F With the annotation of this N-terminal exon the translation exactly matches *F that from U56245 *F thanks *F Nellie # *U FBrf0155327 *a Whitfield *b E. *t 2002.7.23 *T personal communication to FlyBase *u FlyBase error report for CG9428 on Tue Jul 23 07:27:26 2002. *F Date: Tue, 23 Jul 2002 07:27:26 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9428 on Tue Jul 23 07:27:26 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9428 *F Release: 3 *F Gene annotation error *F Gene CG9428 has incorrect exon/intron structure or translation start site. *F Comments: Release 3 has truncated the translation of CG9428 to match the *F translation of the cDNA from AJ401614; CAC14873. *F Complication is that the cDNA BDGP-DGC:RE1584 AY071095; AAL48717 is an exact *F match for the longer translation. Also if you extend the N-terminal *F translation of the cDNA in AJ401614; CAC14873 it also matches BDGP-DGC:RE1584. *F So I wonder if the original translation that exactly matched BDGP-DGC:RE1584 *F should be reinstated as the third source reinforces this N-terminus. *F correct translation? MSATATMSQE QTQDVDHHAL LVAKIVSMVV *F AY071095; AAL48717 MSATATMSQE QTQDVDHHAL LVAKIVSMVV cDNA BDGP-DGC:RE1584 *F AE003790; AAM70819 MSQE QTQDVDHHAL LVAKIVSMVV release 3 translation *F AJ401614; CAC14873 ATATMSQE QTQDVDHHAL LVAKIVSMVV independent cDNA *F thanks *F Nellie # *U FBrf0155328 *a Whitfield *b E. *t 2002.7.24 *T personal communication to FlyBase *u FlyBase error report for CG10052 on Wed Jul 24 08:35:12 2002. *F Date: Wed, 24 Jul 2002 08:35:12 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG10052 on Wed Jul 24 08:35:12 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG10052 *F Release: 3 *F Gene annotation error *F Gene CG10052 has incorrect exon/intron structure or translation start site. *F Comments: Rx translation has been much improved (AE003452; AAF46639) but an *F old problem remains at an internal exon-intron splice site. *F Eggert et al., Proc. Natl. Acad. Sci. U.S.A. 95:2343-2348(1998) translation in *F AJ223300; CAA11241 has a frameshift: *F release 3 PLLSALPGFLSHPQTVYPSYLTPPLSLAPGNLTMSSLAAMGHHHAHNGPPPPHVGHGGHG *F Eggert PLLSALPGFLSHPQTVYPSYLTPQPGARKSDHEQS--GGHGPPPCPQWAAAPHVGHGGHG *F but also you have an incorrect splice site relative to this sequence *F release 3 VRVQFIPPFIFQVWFQNRRAKWRRQEKSESLRLGLTHFTQLPHRLGCGASGLPVDPWLSP *F Eggert VRV--------QVWFQNRRAKWRRQEKSESLRLGLTHFTQLPHRLGCGASGLPVDPWLSP *F thanks *F Nellie # *U FBrf0155329 *a Whitfield *b E. *t 2002.7.26 *T personal communication to FlyBase *u FlyBase error report for CG9325 on Fri Jul 26 05:47:16 2002. *F Date: Fri, 26 Jul 2002 05:47:16 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG9325 on Fri Jul 26 05:47:16 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG9325 *F Release: 3 *F Gene annotation error *F Gene CG9325 has incorrect exon/intron structure or translation start site. *F Comments: Comparing translation of hts from genome project AE003796; AAF57565 *F and that of two independent sequences: *F Yue and Spradling, Genes Dev. 6:2443-2454(1992) \- L05016; AAA28643. *F Ding et al, Proc. Natl. Acad. Sci. U.S.A. 90:2512-2516(1993) \- AF151708; *F AAB59182 *F it seems the genome project is missing an N-terminal exon that encodes the *F first 33 residues *F 'MTEVEQPPQN GIDPTAGEDD DNSKARPADI EQD' *F The exon is there in the DNA sequence it just isn't annotated in the CDS for hts *F thanks *F Nellie # *U FBrf0155330 *a Whitfield *b E. *t 2002.7.26 *T personal communication to FlyBase *u FlyBase error report for CG5058 on Fri Jul 26 06:26:45 2002. *F Date: Fri, 26 Jul 2002 06:26:45 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG5058 on Fri Jul 26 06:26:45 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG5058 *F Release: 3 *F Gene annotation error *F Gene CG5058 has incorrect exon/intron structure or translation start site. *F Comments: One of the intron-exon splice sites in the genome project *F translation (AE003801; AAF57782 and AE003801; AAF57784) is just 2 residues *F short compared to full length translation from: *F Bray et al, Genes Dev. 3:1130-1145(1989) \- X15657; CAA33692 *F and partial translation that covers the splice site: *F Dynlacht et al, Genes Dev. 3:1677-1688(1989) *F ELF1_DROME KNSVGLVGCIEEVSHNAIAVYWNPLESSAKINIAVQCLSTDFSSQKGVKGLPLHVQIDTF *F genome trans KNSVGLVGCIEEVSHNAIAVYWNPLESSAKINIAVQCLSTDFSSQKG--GLPLHVQIDTF *F thanks *F Nellie # *U FBrf0155331 *a Whitfield *b E. *t 2002.7.28 *T personal communication to FlyBase *u FlyBase error report for CG6536 on Fri Jul 26 07:06:21 2002. *F Date: Fri, 26 Jul 2002 07:06:21 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6536 on Fri Jul 26 07:06:21 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6536 *F Release: 3 *F Gene annotation error *F Gene CG6536 has incorrect exon/intron structure or translation start site. *F Comments: The translation currently in SP Q9V817 is taken directly from Brody *F and Cravchik A, J. Cell Biol. 150:F83-F88(2000). *F Fig 3 *F (http://www.jcb.org/content/vol150/issue2/images/large/JCB0004032.f3.jpeg) *F gives an alignment of the Methuselah family members. *F The new genome project translation adds 60aa near the C-terminus that destroys *F the family alignment. Maybe these residues are valid but I do wonder? *F thanks *F Nellie # *U FBrf0155332 *a Whitfield *b E. *t 2002.7.26 *T personal communication to FlyBase *u FlyBase error report for CG7761 on Fri Jul 26 07:28:56 2002. *F Date: Fri, 26 Jul 2002 07:28:56 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG7761 on Fri Jul 26 07:28:56 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG7761 *F Release: 3 *F Gene annotation error *F Gene CG7761 has incorrect exon/intron structure or translation start site. *F Comments: new translation (AE003812; AAF58174.3) has reduced a 477 residues *F translation down to just 37 residues. *F Note in the xml reads 'assume that new one is better' but I am a little *F concerned about this as there is a corresponding Berkeley cDNA *F GH09755(AY058328; AAL13557) that also produces a 477 residue product. *F Should I trust the new translation as the cDNA is also in error, or not? *F thanks *F Nellie # *U FBrf0155333 *a Whitfield *b E. *t 2002.7.26 *T personal communication to FlyBase *u FlyBase error report for CG13158 on Fri Jul 26 07:44:55 2002. *F Date: Fri, 26 Jul 2002 07:44:55 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG13158 on Fri Jul 26 07:44:55 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG13158 *F Release: 3 *F Gene annotation error *F Gene CG13158 has incorrect exon/intron structure or translation start site. *F Comments: Genome project translation (AE003822; AAF58519) is missing the *F penultimate exon compared to the translation provided by Hugh Robertson. *F O49A_DROME SYMMLFASVAAQFYVVSSHGQMLIDLSTNLAKAAFESKWYEGSLRYKKEILILMAQAQRP *F AAF58519 SYMMLFASVAAQFYVVSSHGQMLIDL---------------------------------- *F O49A_DROME LEISARGVIIISLDTFKILMTITYRFFAVIRQTVEK *F AAF58519 \------------------LMTITYRFFAVIRQTVEK *F thanks *F Nellie # *U FBrf0155334 *a Ashburner *b M. *t 2002.7.3 *T personal communication to FlyBase *u *F To: gm119@gen.cam.ac.uk *F Subject: new genes *F Cc: ma11@gen.cam.ac.uk *F From: Michael Ashburner (Genetics) *F Date: Wed, 3 Jul 2002 15:43:45 \+0100 *F \*a Dana\lyso *F \*g AB074814; BAB93535 *F > *F \*a Dana\CG6428 *F \*M CG6428 *F CG6428 protein sequence. This match suggests that CG6428 may be a fusion *F ma20020703. *F >BAB93535.1 *F >CG6428|FBgn0029689|pp-CT20046|FBan0006428 GO:lysophospholipase (GO:0004622) *F mol_weight=69176 located on: X 4A2-4A2; *F Length = 631 *F Score = 1900 (673.9 bits), Expect = 1.3e-197, P = 1.3e-197 *F Identities = 366/404 (90%), Positives = 387/404 (95%) *F Query: 1 MRGNRTVKVSSNSLDAFDSPNVPPLARIGIDVNVDYRLIFRPCSIERFCVHLNLDENVGL 60 *F MRGNRTVKVSSN+LDAFDSPNVPPLARIGIDVNVDYRLIFRPC+IERFCVHLNLDENVGL *F Sbjct: 228 MRGNRTVKVSSNALDAFDSPNVPPLARIGIDVNVDYRLIFRPCTIERFCVHLNLDENVGL 287 *F Query: 61 LRIFPSISHSTFRAFLAPPIRGVVLQSFGSGNIPSNRKDLIEELRAADERGVIIINCTQC 120 *F LRIFPSISHSTFRAFLAPPIRGVVLQSFGSGNIPSNRKDLI+ELRAA ERGVIIINCTQC *F Sbjct: 288 LRIFPSISHSTFRAFLAPPIRGVVLQSFGSGNIPSNRKDLIDELRAAGERGVIIINCTQC 347 *F Query: 121 PNGTVAEIYDTGKVLCDVGVIPGYDMTPEAALSKLAYVIGKTECXLEVKKQMMQSNLRGE 180 *F PNGTVAEIYDTGKVLCDVGVIPG+DMTPEAAL+KLAYVIGK E L++KKQMMQS+LRGE *F Sbjct: 348 PNGTVAEIYDTGKVLCDVGVIPGFDMTPEAALAKLAYVIGKEEWSLDMKKQMMQSSLRGE 407 *F Query: 181 LTSVKAAKIEDYDLVDAVARSLHLSSPQELDQLGATLFPAMINAAVSEGDPKKINNLKAY 240 *F LTS+KA K+EDYDLVDAVARSLHLSSPQELDQLG TLFPAMINAAV+EGDPKKINNLKAY *F Sbjct: 408 LTSLKAPKMEDYDLVDAVARSLHLSSPQELDQLGETLFPAMINAAVAEGDPKKINNLKAY 467 *F Query: 241 GADLSGINHDQRTALHLACQLGSVNIVKYLLNNGVSVHVRDRYDRTPLLEAVSTDNHEII 300 *F GADLSG NHDQRTALHLACQLG+V IVKYLL NGVSVHVRDRYDRTPLLEAV+TD+HEII *F Sbjct: 468 GADLSGTNHDQRTALHLACQLGNVEIVKYLLQNGVSVHVRDRYDRTPLLEAVATDSHEII 527 *F Query: 301 QLLINCGAHLTGSSRAVGEQLCAAAARGSIVRIKSYQLAGADLSQADPSGLTALHVAALH 360 *F QLLINCGAHLTGSSRAVGEQLCAAAARGS++R+KSYQ AGADL+Q+DPSG TALHVAALH *F Sbjct: 528 QLLINCGAHLTGSSRAVGEQLCAAAARGSMIRLKSYQYAGADLAQSDPSGRTALHVAALH 587 *F Query: 361 GHPEVILYVLPYFENPNEKDMLGLTPWDYAERGGHPAVLEALKS 404 *F G PEV+ YVLPYFENPNEKDMLGLT DYAERGGH AV+E LKS *F Sbjct: 588 GFPEVVQYVLPYFENPNEKDMLGLTAMDYAERGGHAAVMEVLKS 631 # *U FBrf0155335 *a Langlais *b K. *t 2002.7.30 *T personal communication to FlyBase *u FlyBase error report for CG4154 on Tue Jul 30 10:16:22 2002. *F Date: Tue, 30 Jul 2002 10:16:22 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: langlais@ohsu.edu *F Subject: FlyBase error report for CG4154 on Tue Jul 30 10:16:22 2002 *F Error report from Kristofor Langlais (langlais@ohsu.edu) *F Gene or accession: CG4154 *F Release: 3 *F cDNA or EST error *F Gene cDNA sequence: *F >CG4154|splice variant 1 *F ATGTACGGACTGCTGCTGGAGAACCTCTCCGAGTACATCAAGTCCGTTTACGGCGAGGAGAAATGGGAGGACATCCGGCG *F ACAGGCTGGCATCGATTCGCCCTCCTTCAGCGTCCATCAGGTCTATCCCGAGAATCTGCTGCAGAAGCTGGCGAAAAAGG *F CTCAACAGGTTCTGGGCGTTTCTGAGCGGGATTTCATGGACCAGATGGGCGTCTACTTCGTGGGCTTTGTGGGCCAGTAT *F GGCTACGATCGTGTCCTTTCCGTCCTGGGCCGCCACATGCGCGACTTTCTCAACGGACTGGACAACCTGCATGAGTACCT *F GAAGTTCTCGTATCCGCGGATGAGAGCTCCCAGCTTTATCTGCGAAAACGAAACGAAGCAGGGTCTGACCCTGCACTATC *F GTTCCAAGCGTCGAGGATTCGTCTACTACACGATGGGTCAAATCCGAGAGGTGGCACGGTATTTCTATCACAAGGAGATG *F CATATTGAGCTCGTTCGGGAAGAGATCCTCTTTGACACGGTCCATGTGACCTTCCAACTGACCTTCGATAATCGGGCCTT *F CACACTTGCATCCTTGGCCATGACCCGGGAGGAGAAACATCTGCCCATCAGTGCTCATGTGCTGTTTGAGATATTTCCCT *F TTTGCATGGTTTTTGGTGCTGATATGGTTGTGCGTAGTATTGGTAACTCTTTGATGGTGATTTTACCGGAACTTCTGGGA *F AAAAAAATTACGGCCTGGTTCGATCTAGTTCGTCCCTTAATTGCATTCAAGTTTCAGACTATACTCAATCGTACTAACAA *F CATTTTCGAGCTGGTCACTGTGGATCCAGTTACGGAGAGATTCGATGTGCAAAACGAGGACTTACTGCAGCATGAAGATG *F GCAGCGAACCGGAAAAGTCTCTTAGATTAAAAGGTCAAATGGTTTACATGGAGAACTGGCGCATGATTATGTTTCTGGGT *F ACTCCCGTAATGCCGGATCTGACCTCACTAATAACCACAGGTCTATATATCAACGATCTGTCCATGCACGACTTCAGCAG *F AGATCTCATGCTGGCGGGCACTCAACAATCGGTTGAACTGAAGTTGGCACTGGATCAAGAACAGCAGAAGTCAAAGAAGC *F TAGAGGAGTCCATGAGATTGTTGGATGAGGAGATGCGTAGAACGGATGAGCTGCTGTATCAGATGATACCCAAACAGGTG *F GCTGATAGGCTCCGACGGGGCGAGAATCCCATTGATACCTGTGAGATGTTCGATAGCGTTTCCATCCTGTTCTCAGACAT *F CGTTACCTTCACCGAGATTTGCAGCCGCATTACTCCGATGGAGGTAGTGTCCATGCTGAACGCTATGTACTCCATTTTCG *F ATAAGCTGACGGAGCGGAATTCAGTGTACAAGGTGGAAACTATTGGTGATGCGTATATGGTGGTGGCGGGTGCTCCGGAT *F AAGGATGCCAATCACGCCGAGCGAGTCTGTGATATGGCCCTGGATATGGTGGATGCGATAACCGATCTAAAGGATCCCTC *F AACGGGCCAGCACTTGAGGATACGTGTGGGCGTCCACTCGGGCGCAGTGGTGGCGGGCATCGTGGGCCTTAAGATGCCGC *F GTTACTGTCTCTTTGGCGATACGGTCAATACCGCCTCACGAATGGAGTCCACCAGCATCGCCATGAAGGTGCACATATCC *F GAGTCTACGAAGGTTCTTATTGGACCCAACTACAAGATCATCGAGCGCGGTGAGATCGATGTAAAGGGCAAGGGCACCAT *F GGGCACATACTGGCTGGAGGAACGAGAGAACCGGCTGCCTCTGCAACTAACCGCTGCTCTGCAAGTTCATCCGCTCTCAC *F CTGTCCCACCGACGCCCACACCCAAGACGAAGGCCATCATGCCTCCTGTATCCAAGCCGTTGACTCCGATGATGCCCGTT *F TCAGTTTCCCTGGCAGCTTCCATGCCCACCAGCAACGTTCCTGCTGTGGATGTGATGGCTTCATCATCCAGCATTTCCGG *F TTTGGCACTAACAGCCGCTGCAGCGGCCCACATGTCGCTACATCACCAGGCGGTGGTGGCGGAGGCATTAACTGGAGCTT *F CAGTTGAAGTGGCACTACCATCAGTAGCTTCTGGCGCAACAGGAGCGGCAGCGGGTGGAGGTGCACCGTCGGATGACCGC *F AACAGTCGCATCTACTCCCCCGTGACCTTCAAGGATGTGGCTCGACGAAGCGTAGCCAATTCTCCGGTAAGGAGCTGTGC *F TCAACCGGATCAGGAGAGACGTCGCGAGTCCCGCTCCAATTCCACGGGCCACGTGTTTATGCGAACTCCTTCGGAAATTT *F TCGGCTCTCTCATCCTGGACACCGAGGAGTTTCTCGAGGATCTGCAAATCTCACGTTCCTCCCTGGCAAACAATAACAAC *F AATCAGAGCCCCTGCGGTTTTAGCCCAACTCCTCCATTCCGGATTGGCAGCGCACCGCCCAAGCCGAGACCTAGTAATCC *F GGATAAATTTACACCGGAGGAACTGGCCGCCATGGATCAACTGACGCCTCCGTCAACGGCACCAGCCAGAGAAACGGCCA *F GCTGCAGCAGTGCATCATTGGATCGCGACAAGGCGACCAAGCTGAACAGCAGTCTGGATGCAACCACTCCAACTGCGTTG *F GCTGCTGTGGTATGTCCAATGAGAACCAAGTCACCGCCAATGGCAGTGGCTCCAGTGATGCACATGGTACAGGCTAGCAC *F CAGCAAAGGCGACGGCCAACGACCAGGCTCGAAGGATTCAGTATCATCCATTTCCCTGCACTCACCTCCTCCGCATCGAT *F CTAACTCGGCACCAGCCAGGTGA *F Protein sequence: *F >CG4154|protein variant 1 *F MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQY *F GYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEM *F HIELVREEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLG *F KKITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLG *F TPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQV *F ADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPD *F KDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHIS *F ESTKVLIGPNYKIIERGEIDVKGKGTMGTYWLEERENRLPLQLTAALQVHPLSPVPPTPTPKTKAIMPPVSKPLTPMMPV *F SVSLAASMPTSNVPAVDVMASSSSISGLALTAAAAAHMSLHHQAVVAEALTGASVEVALPSVASGATGAAAGGGAPSDDR *F NSRIYSPVTFKDVARRSVANSPVRSCAQPDQERRRESRSNSTGHVFMRTPSEIFGSLILDTEEFLEDLQISRSSLANNNN *F NQSPCGFSPTPPFRIGSAPPKPRPSNPDKFTPEELAAMDQLTPPSTAPARETASCSSASLDRDKATKLNSSLDATTPTAL *F AAVVCPMRTKSPPMAVAPVMHMVQASTSKGDGQRPGSKDSVSSISLHSPPPHRSNSAPAR *F Comments: Intron-exon predictions incorrect. Splice variant 2 sent on separate *F form. # *U FBrf0155336 *a Langlais *b K. *t 2002.7.31 *T personal communication to FlyBase *u FlyBase error report for CG4154 on Wed Jul 31 14:20:18 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 31 Jul 2002 14:20:18 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: langlais@ohsu.edu *F Subject: FlyBase error report for CG4154 on Wed Jul 31 14:20:18 2002 *F Error report from Kristofor Langlais (langlais@ohsu.edu) *F Gene or accession: CG4154 *F Release: 3 *F cDNA or EST error *F Gene cDNA sequence: *F >correct splice variant 2 *F ATGTACGGACTGCTGCTGGAGAACCTCTCCGAGTACATCAAGTCCGTTTACGGCGAGGAGAAATGGGAGGACATCCGGCG *F ACAGGCTGGCATCGATTCGCCCTCCTTCAGCGTCCATCAGGTCTATCCCGAGAATCTGCTGCAGAAGCTGGCGAAAAAGG *F CTCAACAGGTTCTGGGCGTTTCTGAGCGGGATTTCATGGACCAGATGGGCGTCTACTTCGTGGGCTTTGTGGGCCAGTAT *F GGCTACGATCGTGTCCTTTCCGTCCTGGGCCGCCACATGCGCGACTTTCTCAACGGACTGGACAACCTGCATGAGTACCT *F GAAGTTCTCGTATCCGCGGATGAGAGCTCCCAGCTTTATCTGCGAAAACGAAACGAAGCAGGGTCTGACCCTGCACTATC *F GTTCCAAGCGTCGAGGATTCGTCTACTACACGATGGGTCAAATCCGAGAGGTGGCACGGTATTTCTATCACAAGGAGATG *F CATATTGAGCTCGTTCGGGAAGAGATCCTCTTTGACACGGTCCATGTGACCTTCCAACTGACCTTCGATAATCGGGCCTT *F CACACTTGCATCCTTGGCCATGACCCGGGAGGAGAAACATCTGCCCATCAGTGCTCATGTGCTGTTTGAGATATTTCCCT *F TTTGCATGGTTTTTGGTGCTGATATGGTTGTGCGTAGTATTGGTAACTCTTTGATGGTGATTTTACCGGAACTTCTGGGA *F AAAAAAATTACGGCCTGGTTCGATCTAGTTCGTCCCTTAATTGCATTCAAGTTTCAGACTATACTCAATCGTACTAACAA *F CATTTTCGAGCTGGTCACTGTGGATCCAGTTACGGAGAGATTCGATGTGCAAAACGAGGACTTACTGCAGCATGAAGATG *F GCAGCGAACCGGAAAAGTCTCTTAGATTAAAAGGTCAAATGGTTTACATGGAGAACTGGCGCATGATTATGTTTCTGGGT *F ACTCCCGTAATGCCGGATCTGACCTCACTAATAACCACAGGTCTATATATCAACGATCTGTCCATGCACGACTTCAGCAG *F AGATCTCATGCTGGCGGGCACTCAACAATCGGTTGAACTGAAGTTGGCACTGGATCAAGAACAGCAGAAGTCAAAGAAGC *F TAGAGGAGTCCATGAGATTGTTGGATGAGGAGATGCGTAGAACGGATGAGCTGCTGTATCAGATGATACCCAAACAGGTG *F GCTGATAGGCTCCGACGGGGCGAGAATCCCATTGATACCTGTGAGATGTTCGATAGCGTTTCCATCCTGTTCTCAGACAT *F CGTTACCTTCACCGAGATTTGCAGCCGCATTACTCCGATGGAGGTAGTGTCCATGCTGAACGCTATGTACTCCATTTTCG *F ATAAGCTGACGGAGCGGAATTCAGTGTACAAGGTGGAAACTATTGGTGATGCGTATATGGTGGTGGCGGGTGCTCCGGAT *F AAGGATGCCAATCACGCCGAGCGAGTCTGTGATATGGCCCTGGATATGGTGGATGCGATAACCGATCTAAAGGATCCCTC *F AACGGGCCAGCACTTGAGGATACGTGTGGGCGTCCACTCGGGCGCAGTGGTGGCGGGCATCGTGGGCCTTAAGATGCCGC *F GTTACTGTCTCTTTGGCGATACGGTCAATACCGCCTCACGAATGGAGTCCACCAGCATCGCCATGAAGGTGCACATATCC *F GAGTCTACGAAGGTTCTTATTGGACCCAACTACAAGATCATCGAGCGCGGTGAGATCGATGTAAAGGGCAAGGGCACCAT *F GGGCACATACTGGCTGGAGGAACGAGAGAACCGGCTGCCTCTGCAACTAACCGCTGCTCTGCAAGTTCATCCGCTCTCAC *F CTGTCCCACCGACGCCCACACCCAAGACGAAGGCCATCATGCCTCCTGTATCCAAGCCGTTGACTCCGATGATGCCCGTT *F TCAGTTTCCCTGGCAGCTTCCATGCCCACCAGCAACGTTCCTGCTGTGGATGTGATGGCTTCATCATCCAGCATTTCCGG *F TTTGGCACTAACAGCCGCTGCAGCGGCCCACATGTCGCTACATCACCAGGCGGTGGTGGCGGAGGCATTAACTGGAGCTT *F CAGTTGAAGTGGCACTACCATCAGTAGCTTCTGGCGCAACAGGAGCGGCAGCGGGTGGAGGTGCACCGTCGGATGACCGC *F AACAGTCGCATCTACTCCCCCGTGACCTTCAAGGATGTGGCTCGACGAAGCGTAGCCAATTCTCCGGTAAGGAGCTGTGC *F TCAACCGGATCAGGAGAGACGTCGCGAGTCCCGCTCCAATTCCACGGGCCACGTGTTTATGCGAACTCCTTCGGAAATTT *F TCGGCTCTCTCATCCTGGACACCGAGGAGTTTCTCGAGGATCTGCAAATCTCACGTTCCTCCCTGGCAAACAATAACAAC *F AATCAGAGCCCCTGCGGTTTTAGCCCAACTCCTCCATTCCGGATTGGCAGCGCACCGCCCAAGCCGAGACCTAGTAATCC *F GGATAAATTTACACCGGAGGAACTGGCCGCCATGGATCAACTGACGCCTCCGTCAACGGCACCAGCCAGAGAAACGGCCA *F GCTGCAGCAGTGCATCATTGGATCGCGACAAGGCGACCAAGCTGAAAAAAATCACTTTCTCCAACAGCAGCAGTCTGGAT *F GCAACCACTCCAACTGCGTTGGCTGCTGTGGTATGTCCAATGAGAACCAAGTCACCGCCAATGGCAGTGGCTCCAGTGAT *F GCACATGGTACAGGCTAGCACCAGCAAAGGCGACGGCCAACGACCAGGCTCGAAGGATTCAGTATCATCCATTTCCCTGC *F ACTCACCTCCTCCGCATCGATCTAACTCGGCACCAGCCAGGTGA *F Protein sequence: *F >correct protein variant 2 *F MYGLLLENLSEYIKSVYGEEKWEDIRRQAGIDSPSFSVHQVYPENLLQKLAKKAQQVLGVSERDFMDQMGVYFVGFVGQY *F GYDRVLSVLGRHMRDFLNGLDNLHEYLKFSYPRMRAPSFICENETKQGLTLHYRSKRRGFVYYTMGQIREVARYFYHKEM *F HIELVREEILFDTVHVTFQLTFDNRAFTLASLAMTREEKHLPISAHVLFEIFPFCMVFGADMVVRSIGNSLMVILPELLG *F KKITAWFDLVRPLIAFKFQTILNRTNNIFELVTVDPVTERFDVQNEDLLQHEDGSEPEKSLRLKGQMVYMENWRMIMFLG *F TPVMPDLTSLITTGLYINDLSMHDFSRDLMLAGTQQSVELKLALDQEQQKSKKLEESMRLLDEEMRRTDELLYQMIPKQV *F ADRLRRGENPIDTCEMFDSVSILFSDIVTFTEICSRITPMEVVSMLNAMYSIFDKLTERNSVYKVETIGDAYMVVAGAPD *F KDANHAERVCDMALDMVDAITDLKDPSTGQHLRIRVGVHSGAVVAGIVGLKMPRYCLFGDTVNTASRMESTSIAMKVHIS *F ESTKVLIGPNYKIIERGEIDVKGKGTMGTYWLEERENRLPLQLTAALQVHPLSPVPPTPTPKTKAIMPPVSKPLTPMMPV *F SVSLAASMPTSNVPAVDVMASSSSISGLALTAAAAAHMSLHHQAVVAEALTGASVEVALPSVASGATGAAAGGGAPSDDR *F NSRIYSPVTFKDVARRSVANSPVRSCAQPDQERRRESRSNSTGHVFMRTPSEIFGSLILDTEEFLEDLQISRSSLANNNN *F NQSPCGFSPTPPFRIGSAPPKPRPSNPDKFTPEELAAMDQLTPPSTAPARETASCSSASLDRDKATKLKKITFSNSSSLD *F ATTPTALAAVVCPMRTKSPPMAVAPVMHMVQASTSKGDGQRPGSKDSVSSISLHSPPPHRSNSAPAR. *F Comments: I believe I sent you a slightly erroneous sequence for splice *F variant 2. The exon-intron structure is the same. The error is a point *F mutation (unique to the clone) I failed to catch and fix in the sequence I *F sent you. It is at position 1984 in the nucleotide sequence: TCACCATCCA *F should be TCATCATCCA. I also found a difference in all of my clones compared *F to the genomic sequence from flybase. At position 657 in the nucleotide *F sequence your genome says: TTTTTGGGGCT and all of my clones say: TTTTTGGTGCT. *F This does not change the amino acid. In the previous splice variant 2 *F sequence I sent, I fixed that sequence to read as your genomic. In the *F current corrected sequence I put it back to TTTTTGGTGCT since it is like this *F in all my clones. So to sum up, there are two changes in this new sequence I *F am sending you. There should be one difference in the protein sequence. *F Terribly sorry for this little mess and please E-mail me for further *F clarification if needed! *F . Thank you very much! *F \-Kristofor # *U FBrf0155337 *a Misra *b S. *t 2002.8.2 *T personal communication to FlyBase *u FlyBase error report for CG3273 on Fri Aug 2 11:24:53 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 2 Aug 2002 11:24:53 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: sima@fruitfly.bdgp.berkeley.edu *F Subject: FlyBase error report for CG3273 on Fri Aug 2 11:24:53 2002 *F Error report from Sima Misra (sima@fruitfly.berkeley.edu) *F Gene or accession: CG3273 *F Release: 3 *F Gene annotation error *F Gene CG3273 has incorrect exon/intron structure or translation start site. *F Comments: Please use the upstream start codon predicted by genscan and genie. # *U FBrf0155338 *a Whitfield *b E. *t 2002.8.15 *T personal communication to FlyBase *u FlyBase error report for CG1339. *F Date: Thu, 15 Aug 2002 15:53:47 \+0100 *F From: Eleanor Whitfield *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report for CG1339 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG1339 *F Release: 3 *F Gene annotation error *F Gene CG1339 has incorrect exon/intron structure or translation start *F site. *F Comments: new translation (AE003840; AAF59215.3) has 1 amino acid *F difference to the translation provided by Hugh Robertson. The error is *F at a splice site so I was wondering if the error it with the *F reannotation or Hughs original prediction. *F G43B_DROME *F LLIAMVLENE-HTSLIIHQKLKPLENMIILDITCDREFVMDYIVTVILTALSLVQYTIST *F AAF59215 *F LLIAMVLENEQHTSLIIHQKLKPLENMIILDITCDREFVMDYIVTVILTALSLVQYTIST *F thanks *F Eleanor # *U FBrf0155339 *a Selvaraj *b A. *t 2002.9.15 *T personal communication to FlyBase *u *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Sun, 15 Sep 2002 08:58:44 \-0700 (PDT) *F To: flybase-updates@morgan.harvard.edu *F Cc: anand@molbio.unizh.ch *F Subject: FlyBase error report for CG15921 on Sun Sep 15 08:58:44 2002 *F Error report from anand selvaraj (anand@molbio.unizh.ch) *F Gene or accession: CG15921 *F Release: 2 *F Missed gene *F Gene cDNA sequence: *F >TTTTAACACAAAATGGGTTGCAAGGCTTGTGGAACAAACTGCCAGTGCTC *F CGCCACCAAGTGCGGTGACAACTGCGCCTGCAGCCAGCAGTGCCAGTGCT *F CCTGCTAGAACGGACCCAAGGACAAGTGCTGCTCCACCAAAAACTAGATA *F CAGGACCCATCTCGAGCTCATCTGTGTGATGAAAGACCCTTTGGCATGAC *F AACGAAATAAAATAC *F Protein sequence: *F >MGCKACGTNCQCSATKCGDNCACSQQCQCSCKNGPKDKCCSTKN *F Comments: This is a metallothinein like protein from the opposite strand of *F CG15921. the translated product of CG15921 doesn`t make a metallothinein like *F protein as it is claimed in GADFLY. # *U FBrf0155340 *a Schneider *b M. *t 2002.9.17 *T personal communication to FlyBase *u FlyBase error report for CG18250 on Tue Sep 17 02:35:32 2002. *F From: FlyBase-error@toy.lbl.gov *F Date: Tue, 17 Sep 2002 02:35:32 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: martina.schneider@medkem.lu.se *F Subject: FlyBase error report for CG18250 on Tue Sep 17 02:35:32 2002 *F Error report from Martina Schneider (martina.schneider@medkem.lu.se) *F Gene or accession: CG18250 *F Release: 1 *F Gene annotation error *F Gene CG18250 has incorrect exon/intron structure or translation start site. *F Comments: The new release lacks an exon between exon 7 and 8. This exon *F (further referred to as exon 8) had been included in the previous release (see *F bottom). *F We sequenced LD11619 and find exon 8 to be present. *F We further checked several cDNAs for the presence of exon 8 and the following *F exon, 9, and found that both are subjected to alternative splicing. *F LD11619 includes 8 and lacks exon 9. *F SD06707 lacks both, exon 8 and 9. *F GH09323 lacks exon 8 and includes exon 9. *F >CG18250:8 generated Thu Dec 20 06:06:29 2001 *F TTGGAGGTGTCGGAGAAGATAGTGTCGCCCGAATCCGTCTCTTCGTCGGC *F TGTTCCCCTGGTTCCGGACGTGGAGGAGGAGATCGAAGAGAGCGTCTCCA *F AGCTGGAGTCCGTCATCAGCAAGACGATTGAGAATACGAAGAATATTAAG *F GAGCTGCCCGTTCTGGGCGAAGCCGACGATGGTGAAGATGAAGAGGAAGT *F GGAACTACAGCAATTGGGAGTTCCATTGGCCGCGGAGATTGTGCCAGACG *F AAACCACCAGTGCGGCGGCCACACGTGCCACGGAAATGGTAAAGCCCGTT *F GATTTGGATGAGCAAGAAATTTCGGTGGACTCCGGCGCTGTGCCGGCTAT *F CGATGTGGAGGCCTCGGCCACGCCCACGCCCTCGATGTCCGCTCGCGAAA *F CACAGAAGCCCTTTTCACCCTATGACGCCACCTCTTCGGTGTCGTCCTCG *F TTCTCGCCCGTCGCATCGATTGCATCTGGCAGCGAAGCTGGTGAAGATTC *F AACTTCGGTGTCGACCCCTCACCTGTCCCCAGCTAACTCACCCACGGTCA *F TGCCCACAGAGCTAGACAGAAATGGCCTAGCCACCACATCATCCTCATCA *F TCTTCATCCGCCTCACCTCCTCCATCGTCATCCGTTCCAACACAAGCCAC *F TCAACCAACCACATCTGCATCATCATTCTCATCATCATCATCAACAACAA *F CAACAAGCACAACAGCAACAGTCTCAACAACAACAACAGCATCATCAACA *F GCAACAACAACTACAACAACAACAACACTGTCTGAATCGCCAAAG # *U FBrf0155341 *a Yu *b J. *t 2002.9.17 *T personal communication to FlyBase *u *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 17 Sep 2002 12:12:05 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: jy64@cornell.edu *F Subject: FlyBase error report for CG7719 on Tue Sep 17 12:12:05 2002 *F Error report from Jiangtao Yu (jy64@cornell.edu) *F Gene or accession: CG7719 *F Release: 3 *F Assembly error *F Comments: There is an allele under this gene CG7719, named KG01447, should be *F put under Pk61C instead of this Pk91C. # *U FBrf0155342 *a Whitfield *b E. *t 2002.10.18 *T personal communication to FlyBase *u FlyBase error report for CG6488 on Fri Oct 18 06:35:53 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 18 Oct 2002 06:35:53 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: eleanor@ebi.ac.uk *F Subject: FlyBase error report for CG6488 on Fri Oct 18 06:35:53 2002 *F Error report from Eleanor Whitfield (eleanor@ebi.ac.uk) *F Gene or accession: CG6488 *F Release: 3 *F Gene annotation error *F Gene CG6488 has incorrect exon/intron structure or translation start site. *F Comments: In the reannotation differences between genomic DNA CG6488 and cDNA *F SD07339 were not resolved. *F I believe that cDNA SD07339 could represent a splice variant of CG6488, for 2 *F reasons. *F 1) *F Upstream translation from cDNA sequence init-Met does not match the genomic *F DNA translation of CG6488 *F 2) *F Mapping the cDNA back onto the genomic DNA shows that the cDNA init-Met is the *F first in-frame Met for that exon. *F Why was a second isoform not annotated during the reannotation, or maybe the *F genomic DNA annotation is in error and should match the cDNA? *F Please let me know which is true. *F thanks *F Nellie # *U FBrf0155343 *a Craig *b C. *t 2002.11.4 *T personal communication to FlyBase *u FlyBase error report for CG6173 on Mon Nov 4 10:29:31 2002. *F From: FlyBase-error@hedgehog.lbl.gov *F Date: Mon, 4 Nov 2002 10:29:32 \-0800 (PST) *F To: flybase-updates@morgan.harvard.edu *F Cc: crcraig@artsci.wustl.edu *F Subject: FlyBase error report for CG6173 on Mon Nov 4 10:29:31 2002 *F Error report from Caroline Craig (crcraig@artsci.wustl.edu) *F Gene or accession: CG6173 *F Release: 2 *F cDNA or EST error *F Gene cDNA sequence: *F ggcacgagcccacttgagatttagattcagattctgattcggattgagattcggtttcggttttgttacttaagatcaa *F cagttcccaggagcggaaatcggaacagtgcggcgaattaaagattaaagtggcaacaagcgatcaaagaaggcgctcc *F gactgcgtgggagtacgagatcacggatcacgaggcgtggagccagtgcatccgaaagtgtttccattaccattgcaat *F caccgaggatcggagcgtttcagatgggcagcatgcaagtggcgctgctggcgctgcttgttctcggccagctattccc *F aagcgccgtggccaatggatcctcctcctatagttccacctccacatccgcatcgaatcagctgcagcgacagaagctg *F gcacactggttccgggatagcaatgatgttaaggataagatcctggagctgcaatgcctggcgaagtgtggcagcaatc *F ccacaaccaaagctggacgggaacagtgcctgaacaagtgcatccaggagcttttgctgggacccagagccggcagttg *F ccccaaaattggaaggcaatcgcgtgccagactctcctgcctggacaactgtcagtacgatcatgaatgcccagaggtg *F cagaagtgttgtccctccagttgcggacccatgtgcgtggaacctctcggcgttaggaacaacacacagcttccgccca *F taccgaagattttgtatttccggagatcgcgaggtcatgctgtcgatctgaagatcgagtcctcgctactggtctacta *F cttccatgtggaggtaagatcccacataggacggcattttgcagccagaaaactgggtccttggcaatggcagaaggtg *F gagaagaccatggaggagaacatcggacacagcaagcatacttacatcttctttcacatgcgacctggtcggtggtatg *F aggttcgagtggcagccgtaaacgcctacgggttccgtggata! *F ttccgagccaagcgatccctttccctcgacgggcaacccaaagcccccaaagtctccgaacgattcgaagatcatcggc *F aagcagttcgatggacgctacatgacccttaagctggtgtggtgcccctccaagtccaacctgcctgtcgagaagtaca *F agatcacctggtcattgtacgtaaacagtgccaaggcctcgatgattacgaacagctcctacgttaaggatacacacca *F gttcgaaatcaaggaactgctacccaactcctcgtactacatccaagtgcaggccatatcctacctgggttcgcgtcgc *F ctcaagtccgagcagtggtcgatgctgttcaacacgacgctgcaacctctggagccaattacaccgcttcagtgctccg *F ggaatggcaataggcgacggcatcatcacactagcagctctatgagctcggaacgggccacaacctcggagccagttgc *F cctcaatgaagtttcgcccaccattacaaaccggacatcggccgccgccacgtatgaagtgggtttccggttaaaccgg *F aagttcggcatgattgtgcagattctgggcttccagccacacaaggagaaggtctatgaactgtgtccccaggagacga *F actgcgagcagcgagagttccgcgcgattcgcgccaaaaaagacccgctggagttcagcaaactgaagtacaacaccac *F gtatgtgctgaggattccccgttccagtcccaattccgtgttggacgactccagaaatgtctttaccttcaccacgcct *F aaatgtgaaaatttccgcaagagatttcccaagctgcagatcaagtgcagcgactagccttctctgtatgggctcaacg *F cactgctgaccatgagattacattagattgtaaataattgtatgtaaaacttaacttttacttatcgttaggcctaaga *F acaaacaacaagaaactaaaaaaaaaaaaaaaaaaaa *F Comments: This is the cDNA sequence submitted by Rugarli et al., AF342988. *F The cDNA sequence in the clot GH04611 contains an extra C at position 655. *F Rugarli et al are in agreement with genomic and BAC sequence. GH04611 is not. # *U FBrf0155344 *a Kovalick *b G. *t 2002.11.6 *T personal communication to FlyBase *u FlyBase error report for CG10651 on Wed Nov 6 12:09:17 2002. *F From FlyBase-error@ogre.lbl.gov Wed Nov 06 20:18:56 2002 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Wed, 6 Nov 2002 20:18:56 \+0000 *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 6 Nov 2002 12:09:17 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG10651 on Wed Nov 6 12:09:17 2002 *F Content-Length: 2488 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG10651 *F Release: 3 *F Gene annotation error *F Gene CG10651 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG10651 *F ctagtccttaactattgtattgaaatttaaatctgggttgtctggtagcgttggcaacacatcgtttggatcgtacgtt *F ggaagtgtgaacacggtggtctcgaattcataatcgttttccatattctcttcttgaccatcgttataatcattttcag *F gttcgacgaaaagtgagccaccattgcaggtcttcacgagctcatctttgtggcaaagattcttgtattgcttattgct *F tcccttcatgcactctgaagcggcttcttcgtcggtgtcctcgtagacgggattatcctcctcctcacagagactcact *F ccgcagttgtagacgcactttagcaactggtggaccagtgctggccgaacatactcaactatggcgcagcccacgcgat *F tagctcgatccctcaatacctgaaagtagtttttggacagttcctgctgatttttagtccatgagaagaacagcttctg *F cacttcatctttactattggggtcaaaccagtgatcgagttgcttcctcagggcctcctttttcgtggtcatgcagaag *F tacttttccagcgaatagctgacttccgcatgaccgtaatttggggttatggcgcactgatctcggattggctcacaac *F gacgtaccagaccgtcggccactttggccagttccggatcccattcgatcgtggtcatccttgccgccttcggattctg *F atccattccgccggcgaacttgttgcgatattcattgtgcttgtccacgatgagggctatcatatcccagctcattttc *F accatggctgcggcttgtttgggacaagtggattcaaaatttccattgtcatcgcagagcacgtgttgtcctcggcaca *F ggtcagctttgcaccatttatctgaagcgcctgtatcttgaatatataatgtggagaacagcagccagatgcactttat *F cat *F Protein sequence: *F >CG10651 *F MIKCIWLLFSTLYIQDTGASDKWCKADLCRGQHVLCDDNGNFESTCPKQAAAMVKMSWDMIALIVDKHNEYRNKFAGGM *F DQNPKAARMTTIEWDPELAKVADGLVRRCEPIRDQCAITPNYGHAEVSYSLEKYFCMTTKKEALRKQLDHWFDPNSKDE *F VQKLFFSWTKNQQELSKNYFQVLRDRANRVGCAIVEYVRPALVHQLLKCVYNCGVSLCEEEDNPVYEDTDEEAASECMK *F GSNKQYKNLCHKDELVKTCNGGSLFVEPENDYNDGQEENMENDYEFETTVFTLPTYDPNDVLPTLPDNPDLNFNTIVKD *F Comments: CG10651 is has sequence similarity to SCP/TPX-1/Ag5/PR-1/Sc *F proteins. In vespid wasps, Ag5 proteins have a sequence containing 4 *F conserved cysteines in a domain N-terminal to the SCP/TAPS domain. The four *F conserved cysteines participate in disulfide bonding. A similar domain with *F four conserved cysteines is also present in some Drosophila proteins with an *F SCP/TAPS domain. These proteins include Agr, Agr2, CG9400, CG6628, CG8072, *F CG5106, CG5207, CG10284, CG17210, CG3640, CG9822, CG13569, and CG17575. An *F sequence with similarity to this N-terminal domain lies upstream of the *F CG10651 SCP/TAPS domain. The sequence is interrupted by an apparent phase 0 *F intron. A phase 0 intron with a similar location is present in Agr, CG3640, *F CG9822, CG17575, CG6628, CG8072, CG5106, CG5207, CG10284, and CG17210. # *U FBrf0155345 *a Kovalick *b G. *t 2002.11.6 *T personal communication to FlyBase *u FlyBase error report for CG11977 on Wed Nov 6 12:39:58 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 6 Nov 2002 12:39:58 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG11977 on Wed Nov 6 12:39:58 2002 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG11977 *F Release: 3 *F Gene annotation error *F Gene CG11977 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG11977 *F atgaatcttttgtgggctatagtgattgtggccgaactgaaatgtcatacgggccatactccacccgattactgcaatg *F cagacatttgtccggccaacaagaagcacatcacctgcggcttcaaattttggtctacgaaatgcggtagaaatcacga *F gggcgtcaggatgagcgattatcgctatgatatcgtgaggaatgtgaacaactttcgacggaagttggaatggggcctg *F gggaatctgcccagagcggtcaaatttaagaacatcaagtgggacgatgagctgtcggtgatggcgatgcgggtctcta *F atcaatgcttgcagcacactttctcaccctgcgtgaatacgttcctctacaaggatgtgggcgagtcctcagatttcgt *F caaggtgcagaacacttccaagggcttcaacgtgatcagcttcctgaatatgtggttcgaataccacaagatgatgaag *F cccagctatgtgaacaactttcccaatatagcccctcaggatcggctcattatcttcgccaatctgatctacgagaaga *F acaagaaaatgggctgcggaatggtgaagtcggggcaggggcgcttcctgacctgcctcttcgataagaaaatcaagcc *F aaaccagaagctttacaccactcggttgaatgatccatttcgcacaaatcggaaaacaaacgaaacagaatcgatccaa *F tcgaatagtagtagcacagaagcaattgtaaatttgaacacagcgtataaatag *F Protein sequence: *F >CG11977 *F MNLLWAIVIVAELKCHTGHTPPDYCNADICPANKKHITCGFKFWSTKCGRNHEGVRMSDYRYDIVRNVNNFRRKLEWGL *F GNLPRAVKFKNIKWDDELSVMAMRVSNQCLQHTFSPCVNTFLYKDVGESSDFVKVQNTSKGFNVISFLNMWFEYHKMMK *F PSYVNNFPNIAPQDRLIIFANLIYEKNKKMGCGMVKSGQGRFLTCLFDKKIKPNQKLYTTRLNDPFRTNRKTNETESIQ *F SNSSSTEAIVNLNTAYK *F Comments: CG11977 has sequence similarity to SCP/TPX-1/Ag5/PR-1/Sc (SCP/TAPS) *F proteins. In vespid wasps, Ag5 proteins have a sequence containing four *F conserved cysteines in a domain N-terminal to the SCP/TAPS domain. The four *F conserved cysteines participate in disulfide bonding. A similar domain with *F four conserved cysteines is also present in some Drosophila proteins with an *F SCP/TAPS domain. These proteins include Agr, Agr2, CG9400, CG6628, CG8072, *F CG5106, CG5207, CG10284, CG17210, CG3640, CG9822, CG13569, and CG17575. A *F sequence with similarity to this N-terminal domain lies upstream of the *F CG11977 SCP/TAPS domain. The sequence is interrupted by an apparent phase 0 *F intron. A phase 0 intron with a similar location is present in Agr, CG3640, *F CG9822, CG17575, CG6628, CG8072, CG5106, CG5207, CG10284, and CG17210. # *U FBrf0155346 *a Kovalick *b G. *t 2002.11.6 *T personal communication to FlyBase *u FlyBase error report for CG30486 on Wed Nov 6 12:47:03 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 6 Nov 2002 12:47:03 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG30486 on Wed Nov 6 12:47:03 2002 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG30486 *F Release: 3 *F Gene annotation error *F Gene CG30486 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG30486 *F tcaattaagctcgcccaattgcagggcattgggattaacatgctcctcgggtgagcagagcccctcatatatggagtgg *F gttcccgcatagcagcgacttcccggatgagcacttgcccggtagaccagctcattggcgatctttccgcgcgaaaagt *F gacataggacaccgtactgatagagtccgctcgtttggccttttcgcagcatcatggcacagccgacatgggcagccaa *F atcttggaccagctgggtgaagtagccgcgacactgtccatccttggccggtttctcggcattgatgtgggccatcgtg *F cagcccttaacatcatccatccaaaactgcaagacaaagtccaaaacggatatgggatccttctccagttgcaaccact *F tggtactgccatatatatacgacacatagctgaactcatccgtattgctgcagtaatcgtctatatagtcacaacgcct *F caaggcaaacttggccaacccggccagttcctcgtgccaccggagtgttgccatccgggaagctggacgcaagtgcgga *F tactgacccttagccacggtattccgaaaatcgtttaggacggccaggagatgggcacgcatgtgcatgggaaacttca *F tgatgatcgcttcactgccacaactctcgtcaaagtcgccataattgcggcaagcgatatgcgttatttccggcagaca *F cagatccttgttgcagtaatccgtagataacgcctcctggctgataaggactattataaggactgaaagaaggtacaac *F at *F Protein sequence: *F >CG30486 *F MLYLLSVLIIVLISQEALSTDYCNKDLCLPEITHIACRNYGDFDESCGSEAIIMKFPMHMRAHLLAVLNDFRNTVAKGQ *F YPHLRPASRMATLRWHEELAGLAKFALRRCDYIDDYCSNTDEFSYVSYIYGSTKWLQLEKDPISVLDFVLQFWMDDVKG *F CTMAHINAEKPAKDGQCRGYFTQLVQDLAAHVGCAMMLRKGQTSGLYQYGVLCHFSRGKIANELVYRASAHPGSRCYAG *F THSIYEGLCSPEEHVNPNALQLGELN *F Comments: CG30486 has sequence similarity to SCP/TPX-1/Ag5/PR-1/Sc (SCP/TAPS) *F proteins. In vespid wasps, Ag5 proteins have a sequence containing four *F conserved cysteines in a domain N-terminal to the SCP/TAPS domain. The four *F conserved cysteines participate in disulfide bonding. A similar domain with *F four conserved cysteines is also present in some Drosophila proteins with an *F SCP/TAPS domain. These proteins include Agr, Agr2, CG9400, CG6628, CG8072, *F CG5106, CG5207, CG10284, CG17210, CG3640, CG9822, CG13569, and CG17575. A *F sequence with similarity to this N-terminal domain lies upstream of the *F CG30486 SCP/TAPS domain. The sequence is interrupted by an apparent phase 0 *F intron. A phase 0 intron with a similar location is present in Agr, CG3640, *F CG9822, CG17575, CG6628, CG8072, CG5106, CG5207, CG10284, and CG17210. # *U FBrf0155347 *a Kovalick *b G. *t 2002.11.6 *T personal communication to FlyBase *u FlyBase error report for CG31296 on Wed Nov 6 13:04:04 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 6 Nov 2002 13:04:04 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG31296 on Wed Nov 6 13:04:04 2002 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG31296 *F Release: 3 *F Gene annotation error *F Gene CG31296 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG31296 *F ctatatggctggcacataagcttgtcgaccgatggataaatcgaggggtaactggaatacccttgcatttagcattggc *F ttattgttttcgtagttttcaccaacagcacaaagtgctgagtatgtgggatccaagcgcttgcaagcagctcctggtc *F tcatagacatccggtagatgggtcgctccacgaagagatcggtgctgaaggcgcacaagaagacgaagttgtggacgga *F gtgcacggtgaatctcatagccgaacagcccacatgggtgttccgatctgccatcagtagtagggcttttgctattcca *F cttttccccaaagttgtcggcatatagacacccattttaatgttgacataatccgcatatctggtccaatgctgtatta *F tccgcaacatgagttctaggtcctcgtagtcgttcaagttacccaaatagtgggtggaacctatattggtacccacgta *F atagaactcctgtgagttcaggcaaaacttgtgcgtggagcacgtgatgaccgccagtcgggctaagtcctccagttcc *F attgagtaactcaatcgggacattcgtgccgccgctgccttgagatacttttgcttcccgctcgccgtataattgcgaa *F actcattgaaggcaattagtaataaattcctgtaggtcgacatggaaagagttctggcattcggtggacacatgacgct *F gaatttcgatgggttattacaggctaggttatttgtaccgcagtaaggcagctggcaaaaatcaaccaacttgctgcat *F ccaaagggaatgaaattgagcagtgctacgaatatgaacccacttagttccat *F Protein sequence: *F >CG31296 *F MELSGFIFVALLNFIPFGCSKLVDFCQLPYCGTNNLACNNPSKFSVMCPPNARTLSMSTYRNLLLIAFNEFRNYTASGK *F QKYLKAAAARMSRLSYSMELEDLARLAVITCSTHKFCLNSQEFYYVGTNIGSTHYLGNLNDYEDLELMLRIIQHWTRYL *F GKSGIAKALLLMADRNTHVGCSAMRFTVHSVHNFVFLCAFSTDLFVERPIYRMSMRPGAACKRLDPTYSALCAVGENYE *F NNKPMLNARVFQLPLDLSIGRQAYVPAI *F Comments: CG31296 has sequence similarity to SCP/TPX-1/Ag5/PR-1/Sc (SCP/TAPS) *F proteins. In vespid wasps, Ag5 proteins have a sequence containing four *F conserved cysteines in a domain N-terminal to the SCP/TAPS domain. The four *F conserved cysteines participate in disulfide bonding. A similar domain with *F four conserved cysteines is also present in some Drosophila proteins with an *F SCP/TAPS domain. These proteins include Agr, Agr2, CG9400, CG6628, CG8072, *F CG5106, CG5207, CG10284, CG17210, CG3640, CG9822, CG13569, and CG17575. A *F sequence with similarity to this N-terminal domain lies upstream of the *F CG31296 SCP/TAPS domain. The sequence is interrupted by an apparent phase 0 *F intron. A phase 0 intron with a similar location is present in Agr, CG3640, *F CG9822, CG17575, CG6628, CG8072, CG5106, CG5207, CG10284, and CG17210. # *U FBrf0155348 *a Kovalick *b G. *t 2002.11.6 *T personal communication to FlyBase *u FlyBase error report for CG16995 on Wed Nov 6 15:04:14 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 6 Nov 2002 15:04:14 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG16995 on Wed Nov 6 15:04:14 2002 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG16995 *F Release: 3 *F Gene annotation error *F Gene CG16995 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG16995 *F atgagcgctcaacaatttgagcaagaagttcttcaagcccacaatttatatcgggccaagcatggtgctcaacccttga *F ctttgagtcccaaactgaatcgattggccacggaatgggccaattatttgctgtccagaaatcgcatggaacatcgaca *F gaacagtggatatggtgagaacatttacatggcctccgggggaaatctgaaaggtgccgatgccgtcagatcctggtac *F gaagagattcgacagtacaattggaattctccgtcgtttcaaggtaacaccggccatttcactcaggtggtctggaaga *F gctccaccgagctaggcgtgggttttgcgaagagtggcagtaccatctatgttgtgtgcaactacaatccccctggcaa *F ctacaacaacttgttcagggagaatgtcgcacccccaatgcaatag *F Protein sequence: *F >CG16995 *F MSAQQFEQEVLQAHNLYRAKHGAQPLTLSPKLNRLATEWANYLLSRNRMEHRQNSGYGENIYMASGGNLKGADAVRSWY *F EEIRQYNWNSPSFQGNTGHFTQVVWKSSTELGVGFAKSGSTIYVVCNYNPPGNYNNLFRENVAPPMQ *F Comments: CG16995 has sequence similarity to SCP/TPX-1/Ag5/PR-1/Sc (SCP/TAPS) *F proteins. However, the SCP/TAPS domain is incomplete. CG16995 is missing the *F N-terminal portion of the domain. A sequence with similarity to this *F N-terminal portion lies upstream of the remaining SCP/TAPS domain within the *F genome. This sequence is interrupted by two apparent phase 0 introns. *F CG4270, CG31482, and CG32313 each have phase 0 introns with a location that is *F similar to the second intron of CG16995. # *U FBrf0155349 *a Saucedo *b L. *t 2002.10.25 *T personal communication to FlyBase *u FlyBase error report for CG1081 on Thu Oct 24 18:15:50 2002. *F From FlyBase-error@ogre.lbl.gov Fri Oct 25 02:26:22 2002 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Fri, 25 Oct 2002 02:26:22 \+0100 *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 24 Oct 2002 18:15:50 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: lsaucedo@fred.fhcrc.org *F Subject: FlyBase error report for CG1081 on Thu Oct 24 18:15:50 2002 *F Content-Length: 2399 *F Error report from leslie saucedo (lsaucedo@fred.fhcrc.org) *F Gene or accession: CG1081 *F Release: 3 *F Gene annotation error *F Gene CG1081 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >AGAATTACTGTTTTCTTTTACACGATCTGATAAATAATACTGGAATTAAA *F ATCGCCGGAAAAGCTCTAGCTGTGATAGAAACACTGGGCCCCAGGAAAAT *F GCCAACCAAGGAGCGCCACATAGCCATGATGGGCTACCGATCAGTGGGCA *F AATCGTCGCTATGCATACAGTTCGTGGAGGGCCAGTTCGTGGACTCCTAT *F GACCCCACCATTGAGAACACCTTCACCAAGATCGAGCGTGTAAAGTCGCA *F AGACTACATCGTAAAGCTCATCGACACGGCGGGCCAGGACGAGTACAGCA *F TATTCCCGGTGCAGTACAGCATGGATTACCACGGCTACGTCCTGGTGTAC *F TCGATAACCAGCCAAAAGTCTTTTGAGGTGGTGAAGATCATATACGAAAA *F ACTGCTCGACGTAATGGGCAAGAAATATGTACCTGTGGTGTTGGTCGGCA *F ACAAGATCGATCTCCACCAGGAACGAACCGTTTCCACGGAGGAAGGCAAG *F AAGCTGGCCGAGTCCTGGCGAGCTGCATTTCTCGAAACGTCCGCCAAACA *F GAACGAGTCCGTGGGAGATATATTCCATCAGCTACTGATCCTCATCGAAA *F ACGAGAACGGCAATCCCCAGGAGAAGAGCGGTTGTCTTGTATCGTAGGGC *F GGCTGAGTAGCTAAAACTACGGCCGAACTGTTGGAAATACGGCAAATCGC *F TATGGCAATACGAAAATCGATTATCTCTTATCTACACAGTTGGGTCAGCG *F TGCATGGCGGGGCCGTACCCTTTGTGCGCAGCAGGAAAATCTAAAAATCT *F AATAGACCTAGTATTCTTAAGACCACACAGCAAGTAACCAATATCAAGCG *F ATCTGAATGCGTCCGCTAAACGCTGCCCAGCAGGTTTTGTGGTTCGCGTC *F TGGCGCAACACAACAGGTTTAAGTTAGTACGTTTTTTTTACAACAACAAT *F TCAGTTACAAATTTCATATACAAATTCTGCAAACTTTTAGTTTCAATTTA *F AATATAATTTTTCAAAATACTAAAAAAAAAAAAAAAAAAA *F Protein sequence: *F >NYCFLLHDLINNTGIKIAGKALAVIETLGPRKMPTKERHIAMMGYRSVGK *F SSLCIQFVEGQFVDSYDPTIENTFTKIERVKSQDYIVKLIDTAGQDEYSI *F FPVQYSMDYHGYVLVYSITSQKSFEVVKIIYEKLLDVMGKKYVPVVLVGN *F KIDLHQERTVSTEEGKKLAESWRAAFLETSAKQNESVGDIFHQLLILIEN *F ENGNPQEKSGCLVS *F Comments: the aa sequence reported for CG1081 currently reported contains a *F different 3' end due to an insertion of 4 bases in the CG1081-RA and CG1081-RB *F transcripts. *F the corrected sequence that i am submitting is what was formerly reported and *F is the ORF predicted from EST GH10361 (the only EST of CG1081 completely *F sequenced). My own sequencing of GH10361 and GH14685 agrees with the ORF *F predicted for GH10361. Additionally, other partially sequenced ESTs in the *F database which cover the 3' end of CG1081 agree with GH10361 and not CG1081-RA *F or CG1081-RB. *F there is a note saying that 'C-terminus differs from SWP:Q9VND8 due to *F frameshift at last splice site; BDGP EST data is very clear that C terminus is *F different.' # *U FBrf0155350 *a Huen *b D. *t 2002.9.22 *T personal communication to FlyBase *u *F From: David Huen *F Reply-To: smh1008@cus.cam.ac.uk *F To: flybase-help@morgan.harvard.edu *F Subject: Genuine duplication or assembly error? *F Date: Sun, 22 Sep 2002 18:41:38 \+0100 *F Hi, *F There appears to be what is either a genuine duplication or assembly error *F in AE003634.2 (NCBI version dated 16 Sept \- we seem to still as a version *F skew between NCBI and BDGP blast server so attempting to report coordinates *F is a non-starter). *F The duplication spans:- *F 1st copy: *F ======= *F C-terminal part of CG5202 to N-terminal of CG31864. *F immediately followed by:- *F 2nd copy:- *F ======= *F C-terminal part of CG31864 to CG12264. *F The sequence of the first copy is listed here so you can place it against *F whichever scaffold you use:- *F GAGATCTGGTTCGTACGCTTCGCCTTCAACGCCTGGCAAAAGATTCTGGCCTTGGGCAAT *F CAACTGGGCACAACATTTGTCTGGGAATTGGACTGCAATGATCCCAATTTAACAAAGTGT *F AGCCAGCTGGTTCATCCCAAAAGCAACTCCACCATCCGACAGACTTCCTTCTCCAAAGAC *F GGTTCCATTTTAGTTTGCGTGTGCGACGACAGCACCGTGTGGCGATGGGATCGCGTCAAT *F TAGGATGAGTGTTTCTAGGCTAAAAGAATTTTCCTTATCCTTCTAAATCCGATACCATAT *F TTATGTAACCATAGTTATTTTCAGTGTAATGTTTACCTTCAATATTAAGTATGTTATTAT *F GCGTAGCATTTATATACAATACTCATTGTATTGACAATTTATGTTAAATTATGTTAAATT *F TTTTAACATTAACCAAGATATTTATTCTATTCTATTACTTTTTTAATGATCAAATAGCTT *F CGCAAATATTATCATCTTATGTTGTGTGAATGGCGTGGGCTCTATCTTTAATGTTTATAT *F TTTAACTAACTAACTTGTACACAATAAAAACTGTGAACACTAAAGATTAAAACCAATATT *F TTTTTAAAGGGTTCTTCAAAAATAACGGTTTTTATCTCAAATGTATCTGGTATTTTCAGC *F CGTTCTTTGAACGGTCACTCGAATCAGCTGCATTCATTTGTTTATATAGCAATTCGCAAA *F CAGTTGGATTTTCCTGTTTAGTTGTTATTCCCATTACTAAAATGCTTACCGACAGAGTTC *F TGGCCACCAAGGAGGCGCTAGTGGTGGCTCTGGGCGACAACTGGGAGCGTTACCGGGCCA *F ACATGAAGAATTGGTTTCGGAGCCGTTGGACTAAGGAGGAATTCGATGCGGAGTCGCGGA *F AAATCCTAACACCTGACAAACTGCACCTGCACAATCAGTTCCTGCTCGCCCTGCTCAACA *F AGATCGACGCCTTTGCTCCCCTGGAGAATCCTCCGGCCGTCCAGACCAGCAGCAGTAGTG *F GCAACCGAAGCAAACGGCGCAAAAGAAGTTCCCGCACCTTCGCCGAACGCCTTAATTTTG *F AGCTCAGCGACGTCCTGGACTTTGTAGCCGAGGACAACATGCAAATCATTCGTCCGCCAA *F CCACGATCGGTATTCCGTCGGACCAGCAACAGCAACAGCTTCAGTCGCAGCGGTACTGCG *F CGCAGGAACTTTTCCTGCCCGACGCGGGATTCATTATGGGACGATTTCTGATCGGTGCCT *F GGGAAATCGGACTTGTCTCCGTGGACGACAATGTCGCCGAGTACGTTGCCATGGCCGTGC *F AAGTTCTGCTCAAGGATCTACTATCGGCAATCATCAAGAAACGAAAGCACTACAAGACCA *F GCGGCGAGGGAAACTTTTACTACGATGTAGGTGCTCCGTTGCGTGATCCTTCGCTGCGCA *F ACACCGTCACCCGGCAGAAGGTCGACGACACGCCGCTGGAACTGGACAAGGAACTGAATA *F CGGCCAACTTTATGCGGCGACAGAACGACGATGTGACCTTCCTCTCCGCCTGCGAAGAAG *F TGTAAGCTCCTGAATAATAATGAGATTTTCATGTGAAACACAAACTTAGGTTTTGTTTCT *F TTCAGCCAACCCACCGAACGCACAGTAATCACCCTCAAGGACTGCCAACTGGCCTTACGG *F GATCGCAACCTGATAGGCTCGCATGCCGTCTACTCTATAAACATGGAACGACTCAATATG *F ATGATGCATTAGCTTATAAGTTCTAATTATTTTATTATATCAGCTTCTGTAAAAATATAT *F ACATTATACGCTAGTGTACCATTAAGTGTTTGGTTCAGCAAAAATTCCGTGGAGGCGGTT *F TGAATGGCAAGGCACCTGGTAGAAATTGATCCGCCTTGAATGCTTCGATTTCCTGCTCGT *F TGAGATCCTCCATGCGACTGTAAACTGAGGACTGGATTTCGTTGGTCGGTTGAGCTTGCT *F GCTGCTGCTGCTGCATCATTTGCTGTTGCTGTTGCATTTGAAATTGTTGTTGCTGCTGCT *F GCATTTGTTGCTGTTGCATCTGCTGCTGCTGATGCATTTGTTGCTGTTGCATCTGTTGCT *F GCTGCTGCATCTGTTGCTGCTGCTGCATCTGTTGTTGATTAGGGAATGCAGATGCTGCTG *F CTTGAGCAAATAATCCGGAGGCAGCTGCCTGCTGCTGCTGTTGTAGTTGCTGAGAAGCCT *F GAGTAAACATTCCGCCCATATTTTGGCTTGCGGCTTGGCCAAAGATTCCCGTCGACTGTG *F GTTGCTGCTGCTGCTGTTGGAAACCACCGAAAGGATTAGGATTCACTGGCTGAGCTTGCA *F CCTGAACCTGCGCCTGGGCGAAAATGTTGCCCCCTTGGAAAGCTTGTGCAGCAGGCTGCA *F GCTGTTGCTGCTGTGGCTGTCCAAAGACACTTTGTGCACCTGTTGCCGCAGCTCCTACAC *F CAAAAATGCTATTCCTTGCATTGGCATTGCTAATCGTTCCGCCGAAAATGCTATTGGTGT *F TGCTGGCTTGGCTTGTGGCATTCCCAAATATATTTCCCGCACTCAGCGTGGGCTTTCCAA *F AAATGGATGCAGATGTATTGGCGGCAGCCGGAGCTGCTGCAGCGAAGGGATTACTGGACT *F GCTGAGGGGCAGCCGACACAGTGCCGCCCTCCGGCGTCTCGTGGATCTTGATCATCATTT *F CCAGGACCTGCGGCGACATCTGAAGCATGGTCTTCATCTTGTTGTTAGCCTCTAGGACCT *F CACGGTTAAACTGTTGGCTGAACTGTTCGAATTGGTTTTGTCGTTTGGCCTCGTAACACA *F TGAACCGCACCTCCTCGAAGGATTGGTCCTGAATAAAGTTGGGGAAATTCGCTTTGTCCT *F TAAAGGGTCCGTAGGCGGACAAAGGCCACATCTTGCCATTGAGGCTGGATTCCATGTCGG *F CTTTGAGATATTGCCTAAAATAATTAAGTTATTAGTTCATCTGCATAGTTTTAATATCAA *F TGTACTCACTTAACATCGAAATGCTCGTTGTTGCACTTTGCGCCATAGCGGCAGGAGCCT *F TGTTGAAAGAACCGGCAGACAACCATATTTAAATTTGATTTTAATTTTTGCCTATTTCCT *F ATATTTATTATTTCAAAACTGCTTCAACTTTTGTACTTTTGTTTATAATTGTCTTACTTT *F GCGTATTGAGTATTCTTTGAGTATTTTATGGCTCACTGAGAAAAATATACCGTGCCTATG *F CGCGCAATTTCGTGAATTCGTAACTTAAGTTCGCTGAATGATTAAGATAATTTTGTAAGC *F AAACATATTTAAGATGAAAACTGGTTTTTGAAAATATAGTTCCTTGTAAATTATACATTA *F AGTGCGAATATATCTGAAAAATATTGGTTTCTTGCACAGATGCTATTCATTTTTCATATG *F TTCAAAGAAATTCAGAAACTTGCTTCTGCATAATCGAATCAGATAATTTTAAAGTGAATT *F CCGGAGTCCTTTGGTATATCTCGTGCCGCCGAATACGGTCCCACTGATTGTGAAATCCGT *F GATAACGAATTTTAACACCAGAATTTCTAGGATTCCATCAGAATGCAAAAAGTGCTGGGA *F CGCATCCAGGCGCAGGTCCTCCGCTCCCGGGCGACGAATGCATTAGCCAATGTGGTTAGA *F CACGAGGCCACCTCCTCCAGGACAGCGGCTAAACCAGCCGCCACATCCAAAGGTAAGCGG *F ATCCTCCAAGATGCACAGTTACCGCTTACCAAAATCGTATTCGTTCGTAGAATTCCGGGA *F GAGACAGGTGCGATTCAACATAAAGAACGAACAGACCGAGGGTAGACCACTTTACCTGGA *F TGCCCAGGCCACCACTCCAATGGACCCACGTGTATTGGACGCTATGCTCCCCTATCTGAC *F CAATTTCTACG *F The first and second copy are identical except in a small central portion *F (nts 1973-2137). *F This central portion is very slightly different:- *F Score = 825 (129.8 bits), Expect = 2.0e-30, P = 2.0e-30 *F Identities = 165/165 (100%), Positives = 165/165 (100%), Strand = Plus *F /Plus *F Query: 1 AGCTTGCTGCTGCTGCTGCTGCATCATTTGCTGTTGCTGTTGCATTTGAAATTGTTGTTG *F 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 172919 AGCTTGCTGCTGCTGCTGCTGCATCATTTGCTGTTGCTGTTGCATTTGAAATTGTTGTTG *F 172978 *F Query: 61 CTGCTGCTGCATTTGTTGCTGTTGCATCTGCTGCTGCTGATGCATTTGTTGCTGTTGCAT *F 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 172979 CTGCTGCTGCATTTGTTGCTGTTGCATCTGCTGCTGCTGATGCATTTGTTGCTGTTGCAT *F 173038 *F Query: 121 CTGTTGCTGCTGCTGCATCTGTTGCTGCTGCTGCATCTGTTGTTG 165 *F ||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 173039 CTGTTGCTGCTGCTGCATCTGTTGCTGCTGCTGCATCTGTTGTTG 173083 *F Score = 751 (118.7 bits), Expect = 4.3e-27, P = 4.3e-27 *F Identities = 157/161 (97%), Positives = 157/161 (97%), Strand = Plus / Plus *F Query: 5 TGCTGCTGCTGCTGCTGCATCATTTGCTGTTGCTGTTGCATTTGAAATTGTTGTTGCTGC *F 64 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 176837 TGCTGCTGCTGCTGCTGCATCATTTGCTGTTGCTGTTGCATTTGAAATTGTTGTTGCTGC *F 176896 *F Query: 65 TGCTGCATTTGTTGCTGTTGCATCTGCTGCTGCTGATGCATTTGTTGCTGTTGCATCTGT *F 124 *F ||| |||||||||| ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 176897 TGC---ATTTGTTGCTATTGCATCTGCTGCTGCTGATGCATTTGTTGCTGTTGCATCTGT *F 176953 *F Query: 125 TGCTGCTGCTGCATCTGTTGCTGCTGCTGCATCTGTTGTTG 165 *F ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 176954 TGCTGCTGCTGCATCTGTTGCTGCTGCTGCATCTGTTGTTG 176994 *F This has the following implications:- *F ============================ *F 1) CG31864 is a fusion between two known genes, CG12264 and CG5202. *F Evidence for the genuineness of CG12264 and CG5202 is strong. *F 2) CG31865 and CG31866 are identical. *F 3) CG18787 and CG18789 are almost identical. The difference lies in near *F the C-terminal end of the genes where the slight sequence difference *F between the copies leads to CG18787 terminating first. I note that the *F sequence viewer from BDGP names both these genes as CG18787: perhaps in the *F current version of AE003634, both sequences are now identical? if this is *F the case, it will be worth reviewing what evidence there is for this *F duplication being there at all. It may well be artifactual. *F Regards, *F David Huen, Dept. of Genetics, Cambridge # *U FBrf0155351 *a Shaffer *b C. *t 2002.11.14 *T personal communication to FlyBase *u FlyBase error report for CG12864 on Thu Nov 14 11:20:31 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 14 Nov 2002 11:20:31 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: shaffer@biology.wustl.edu *F Subject: FlyBase error report for CG12864 on Thu Nov 14 11:20:31 2002 *F Error report from christopher shaffer (shaffer@biology.wustl.edu) *F Gene or accession: CG12864 *F Release: 3 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG12864. *F Comments: Please see shaffer et al. PNAS 99:14332-7 (2002). We have indetified *F the protein product of this gene as a chromosomal protein localized to *F heterochromatin. We have shown by several methods that the protein interacts *F with Heterochromatin protein 1 (HP1). We have also recovered EMS induced *F lethal alleles of this gene, three of which are reported in the paper. Two of *F the three lethal mutations act as suppressors of variegation when tested *F against wm4h. We have named the gene Su(var)2-HP2 and named the protein *F Heterochromatin Protein 2 (HP2). Finally we have identified a second *F transcript that is produced from this locus. This transcript is an *F alternative splice product that connects the end of exon 4 to the beginning of *F exon 7 producing a transcript of 5081 bases (predicted from genomic sequence) *F and matching well to an observed transcript on Northern blots of 5.0 Kb. We *F can detect at least two protein products which match well in size with the *F size predicted! *F from these two transcripts (~175 kDa and ~350 kDa) # *U FBrf0155352 *a Robertson *b H. *t 2002.9.2 *T personal communication to FlyBase *u *F Date: Sun, 01 Sep 2002 22:02:36 \-0500 *F From: Hugh Robertson *F Subject: methuselah family of GPCRs *F To: Millburn_Gillian *F Dear Gillian, *F .. *F for a small part of a manuscript we have almost accepted in Science *F for the A. gambiae genome issue this Fall I have worked up the methuselah *F family of GPCrs. I see that West et al named the genes last year in PNAS in a *F mth-like series, abbreviated mthl in my work .. *F Anyway, the annotations of these proteins remain somewhat poor in the *F public databases, so I'm not sure if they have been updated to what they *F published. Any case, I've also added two members, 13 and 14, by fixing the *F annotation of GH30018 and recognizing RE31310p as a family member, *F respectively. Below is a simple FASTA compilation of the resultant family. *F I've done this pretty carefully with detailed alignments of intron positions, *F etc, and am pretty confident of them from a bioinformatic perspective, and *F several now have full-length cDNAs, but if you have questions about any of *F them please let me know. *F Hugh *F >mth *F MKTLLVLRISTVILVVLVIQKSYADILECDYFDTVDISAAQKLQNGSYLFEGLLVPAILTGEYDFRILPDDSKQKVAR *F HIRGCVCKLKPCVRFCCPHDHIMDNGVCYDNMSDEELAELDPFLNVTLDDGSVSRRHFKNELIVQWDLPMPCDGMFYL *F DNREEQDKYTLFENGTFFRHFDRVTLRKREYCLQHLTFADGNATSIRIAPHNCLIVPSITGQTVVMISSLICMVLTIA *F VYLFVKKLQNLHGKCFICYMVCLFMGYLFLLLDLWQISISFCKPAGFLGYFFVMAAFFWLSVISLHLWNTFRGSSHKA *F NRFLFEHRFLAYNTYAWGMAVVLTGITVLADNIVENQDWNPRVGHEGHCWIYTQAWSAMLYFYGPMVFLIAFNITMFI *F LTAKRILGVKKDIQNFAHRQERKQKLNSDKQTYTFFLRLFIIMGLSWSLEIGSYFSQSNQTWANVFLVADYLNWSQGI *F IIFILFVLKRSTWRLLQESIRGEGEEVNNSEEEISLENTTTRNVLL *F >mthl1 *F MDRSRSSRAASSNQFIRPCGLLTTVILLQTLVSMSLAIEEMSPPPAAPPRPSPPPTVKLNKCCHSGEYLNDGTCIAGS *F EALWLPMVYLVQQQRFFEPHGASPRFLKFLPNTRPTCRKDQTTEIFRSRGANVMLFPNGTLYVRERALMVQPSDYCVD *F WEVAVVCLNDSQPINALEDPDYAANPLVQQEPPKASLRLSKCCGKWGSYNTQLQNCDLQPNHQAAVDGLLRLSPQLPE *F GSYQTSYGLPDCGQPGGYSIAGDWQDAKLDRNTAMLQLPHKNLSAGQYCLEHTQREGEVKIIACQHLFSSAAGAGIHD *F GSIGGTIEQANGQNLQKAVLTGGILVSIVFLSATLVAGFLLPAVHHALHWRCQICYVTCLLFGKILLAIEELSSSLQP *F GSAACHTLAITMQFFFLAAFFWLNTMCFNIWWTFRDFRPSSLERNQEALRRYLYSLYAWGGPLLITFVAACVDQLPET *F TLLRPGFGQLYCWFDNRNLSIFAYFYGPIGLLLCANIALFVSTTHQLTCGLWKRDDVKSSSEKSALGRVCLKLVVVMG *F VTWIADILSWLVGGPHGVWFFTDLINALQGVFIFIVVGCQPQVWTACRRIFCPRLRHDITNTTNGVQHSSSSQGLPSM *F AGGTEITQNTTTTTTTTNTTATHMPSNPAEDEVPEKAPIAPVAPIVKMETIC *F >mthl2 *F MLLSASILIYFLLNLQSSSAEIADCSFYDTVDISEGQRLSNGSYLYEGLLIPAHLTAKYEFKLLANGDKEQVPSHVRG *F CVCKLRTCVRFCCPHDHIMDMGECYANMTTEENELLDPMLNVTLDDGSVVQRHYKKELMVQWDLPKPCDDMFYLDNRD *F IMDEYTLFENGRLLRHYDQVYLDKSEYCLQHRTFGEGNNNSIRIIPHNCLILPSRTGQTVVMITSLICLVLTIAVYLC *F VKKLMNLEGKCFICYMMCLFFGYLFLLLDLWELSLDFCKAAGFLGYFFVMAAFFWLSIISRHYWKCLTNPCASMNIRS *F ERAFLLYSCFAWAMPLALTGVTYLADNVVNNEEWQPRVGDEGHCWIYTKSWSAMVYFYGPMVLLILFNITMFVLTAKH *F IIDSKRTLRKIARNEGRIQKLNSDKQNYTQFLLLFTVMGMSWSFEIFSYLVQREKLWVNIFLVADYFNWSQGVIIFVL *F FILRRKTLVLFKKQIFPKQRAFSRSATQSTIESISQTKRHFNMT *F >mthl3 *F MRIVIGSFTAFLLLLLQNSNAEIPGCDFFDTVDISKAPRFSNGSYLYEGLLIPAHLTAEYDYKLLADDSKEKVASHVR *F GCACHLRPCIRFCCPQYQKMQKSKCYGDMSEDELNKHDPFVNVTLSDGSVVRRHFKEDLIVQSDLAKPGCPRMYFLNH *F ELPGNEFTLFENGSLLRHWDKVELSKREYCVQHLSFKDDSIRIAPHFCPLSSEHSRTWKTVAIVISLICIILTISVYL *F YVEKLRNLHGKCFICYLASLFLGYFFLVLNVWKYSSGFCVTAGFLGYFSVIAAFFWLSVISLTLWNSFSGNSSWLNRF *F LPQNRFLSYNLYAWGMALLLTAITYIADQVVKNEKLRPRVGVGKNCWIYTGDMTVMIYFYGPMLLIIVFNITMFVLTA *F FRIMKVKKEAQNFTQQQKTTNRLNSDKQTYALFLRLFIIMGLSWSLEIISFLLSKNQAWAKAFMVADYFNWSQGTVIF *F LLFVLRPSTLKLLKERIKGGRDEAGASDEHISLQNTKIDPSVF *F >mthl4 *F MRILLIAVLFLLMPKSNAEIPGCDFFDTVDISKAPRFSNGSYLYEGLLIPAHLTAEYDYKLLADDSKEKVASHVRGCA *F CHLRPCIRFCCPQYQKMQKSKCYGDMSEDELNKHDPFVNVTLSDGSVVRRHFKEDLIVQSDLAKPGCPRMYFLNHELP *F GNEFTLFENGSLLRHWDKVELSKREYCVQHLSFKDDSIRIAPHFCPLSSEHSRTWKTVAIVISLICIILTISVYLYVE *F KLRNLHGKCFICYLASLFLGYFFLVLNVWKYSSGFCVTAGFLGYFSVMAAFFWLSVIGIHLRIKFSLASNCLHRLLPE *F NPFRAYNLYAWGIPLIMTAITYTADQVVKNEKLRPRVGVGKNCWIYTGDMTVMIYFYGPMLLLIAFNIIMFVLSAIYI *F YNIKKNVKGLVHKQQTNQQINDQQMFAIFLRLFILMGLSWSFEILSFLLTKQQAWARALMVADYFNWSQGTIIFVLFI *F LKPSILKLIIAGGRQNLPGSHHNSRSKAARYNSTHTACEGSIADPNAYC *F >mthl5 *F MLVKTLGAHFAAGQNAKKCSCCALLISLLCVLLLSLNPLPVTSHVTSAGSSTALSSDPNLVLFNKCCEKFEIHVDHEC *F QQVNETDYFQPMFTSYGGEQNRPVKFKFVIGIPNCGSMQMWPIYHYAGSSDKLVLLDDGRLRHYTNAENEAEERHGIQ *F SDYEEDIAGSLEPLYHDYDKGLYCIDKATSSTGEENVLFANICLARKEIKWSDSNFLLRKILNPIFHGISLVILLVIA *F IIYFILPTLRDLVGNIVTTIAMCLMVSQAADLVRIFTELTSHVSFIVADIILCFSLLAAFFWLNSFGFYIWKTFRSRN *F VFLRVTDGRKYCYYSAYAWGCTATMAALAVFAHFFLDAESYKQEHMVGEQETIGWLGICIFFAPIACTILVNIFFYVT *F TRKLINRRTVYGRIAHKLKANFIMFSLMLLVMSIAWLFLIMSWLQMEGLLYAHIVVNALQTPLLLYICVLRQRHVTFL *F LKKTCCYNEPPSANDWGDELHYMNGNDY *F >mthl6 *F MLLNILAIILVFVISSQSEAVIPGCDYFDTVDISHIPKLNDSYAYEELIIPAHLTGLYTFRQLADGSQEPVKSHLRAC *F ICKLKPCIRFCCPRNKMMPNSRCSDGLTENLKRINPYLKITLEDGTIGKYYLLTDMIVLRYEFRYCEKVVSVQEDQYK *F LYENGSFMIKPDVNWTLSKQWYCLHPRLEDPNSIWILEHVYIPKSMPAVPQVGTISMVGCILTIAVYLYIKKLRNLLG *F KCFICYVFCKFVQYLIWAGGDLNLWNNICSLAGYTNYFFALASHFWLSVMSHQIWKNLRLINRDERSYHFLIYNIYGW *F GTPAIMTAITYLVDWAWEDRPDKLNWIPGVGLYRCWINTYDWSAMIYLYGPMLILSLFNVVTFILTVNHIMKIKSSVK *F SSTQQQRKCIQNNEFLLYLRLSVMMGVTGISEVITYFVKRHKFWRQVLRVPNFFHLGSGIVVFVLFILKRSTFQMIME *F RISGPRRQQPAS *F >mthl7 *F MRLPWVIFCTVLLLIFTNNSNADIPGCNYYDTVDISYIERQNDSYLYDDIEIPASLTGYYEFRQFGDGSITPIEKHLR *F ACVCSVRPCIRICCPAKNFLANGKCDDGLKEELARFKPYIYFTYMDLQARVPLTDMAIIRDEFFDCDEMIYISDFNYF *F LEEDGKFWVTVDLFMEKQDYCLYRHNFDSDFPKSMWIIRHRCTSHISPGSLEILIITMICFVLTIAVYLYIKKLRNVT *F GKCIVCCIVSRFIQCLIMILDHLNLLNGICSPAGYSSHFFRMASNLWLSVISYHTWKVLTSLNRVDPNYRFLRYNAFV *F WSTAAIMTGSIYIVNQIWENDPSKWNWLPLVGFIRCSVKDWHPSVWIYISGPSLALSTFNVAMFALTAIYIRKVKGGI *F NKFTNEEEGRINCINFDSQTYLQFLRLSIVMGLTWIFNVIPYSARLHIFWEWVGIISEYFHSAFGIVLFVLLVLKRST *F WTLMMDS *F >mthl8 *F MAQFCILGVLLILSGTHCSWGFHEETHYPCAFIDTANITGSYGLDGPFVHNWTVIPRHFVAVYDFVIENGIRIPASRH *F LRACVCKTKPCVRICCLRGEIYDLEKRQCLVPVAGVSSLPSHSHMEVELGNGSLRLVKLQPRFSIHVETPCEHMKAVT *F KGSEYVHWTLHENGTISHRGHIFSKHYCFTPLLHGNSTWEWQPLACAPEKLYFVLGVREWTYAICLLIAILSMFIVLM *F VYLMCSEMRNSFYGVAIKAYAICMILGYALLAYLTLHNPANLSNAACRILPSLALMNLVLSFYILSFIAFKLYLSFYG *F VVFTKLMFWLIFTPIVLVAVGWSFFVGFSYYGSRLIFGGDTCWFDPRNWSVMIYFYAPVFVACAISGFFYVLSQIYIR *F DQPDIETEKSFESIEKNRFKSFWKYFGYTAVVWVVCICSFAFNYYWENRSHLNYAVSFCMAFHGFAALYALIGKNQQI *F QNFLRRIDNGEDTCENSVPLSSFG *F >mthl9 *F MVSPLIILLIIWLSVGAKSVEIASINHPCAYAHTVNITDGLRMKDGSYSYAGVVVPPHLMAEYSFKVIDGVEYRAKKH *F LRGCVCLLKPCISFCCPENLVFDAKHWNCTMPHQVRESTHVELTYANRTVDQVRIRDRFVVRTELGCRNKFVDKKHDN *F FWQWDLFENGTLRRDNRLWSTDEYCFSPLEHNPEQWELTPLNCERFQTGYRVWIYAICSIIAIIINIFILSLLGSVRD *F ARKSHYGQLIIYYLLSMIVGYSLLVYLALKNPMKLSHVACRNIGFLAYFCIMLSFVFLAICSLDFLLKFKQKAVRSSV *F RRLSLALAVLAVIGLRFLVSLAQDSKLPKHFKPGMGEDYCWFDVRTWGILIYYYGPIALLLIFSIVCCLKAYFSIYEL *F PPDTQYILGTQLKIVKTHFYAFSAYIVGVFAVWIREIVVYIMARVREHFFIIDFWSGICILGLAIAGFILLLGKNLHV *F KSWWAINVESSQTDLSIINARVYKFDEKGDLKSSDSPYKPTVTSL *F >mthl10 *F MPKKIHQPGGSLYCGVTLLGVLCLVVFRLIPGIPFGTYVMAERDHYHTIDDPNVPCNFYDTVNLTGHRLFPNGSYDYY *F GTIVPAELVGTYDYIHSSLTERIEVREHVRGCVCKFKSCLNICCPWRQVFNSEVDGCIIDHSDNRTWPDPPMLNITFR *F NESTILVNMFTQFAIQSFRPCPKMFSLQPETNNWDDYLLFENGSMLRVDDKLLIRKNEFCMVPTYVNESDMFYTIHPA *F NCDMQDDHSTVKIINSYAMMFSIPFMMLTIAVYLLIPELRNQHGKSLVCYLIGLSVGYSSLCYVQLYQVDATGVTCKV *F FGYTAYFFFMGAYMWLSVISFDLWHNFRGTRGINRFQEKKRFLFYSLYSWGIALVFLAFTYCAQQLTNLPANLKPGIG *F DGVYCWLDMSNWAAMIYFYGPILAIVVANTIMFIMTAIKIHGVQREMARIIASENSTKNLRTEKDKFGLFLRLFLIMG *F ITWLTELISYFVGSDKGWSKLFYISDLANAMQGFLIFMLFVMKKKVKHLITNRCSSVRDGSNQRQSQYSTKTTSSSVA *F NLSLHEKPSVEKPLVISSSVDPQKTTIFR *F >mthl11 *F MGMFRVEYLLLGILVIGVRSRDIPNCDFFDTVQLRESEKLCNGSYRYEDVVIPAKLTGKYDYEIDYDGDRVSVPKHIR *F GCVCKLKTCIRFCCHHKKLMAGNLCSQDVYENLTYEYTLDITQLNGSVIKKHVLNDMVVQQDLPLPCERHYSLDAETS *F TYDMWSLYENGSLFRHFDQRYLSKQEFCLQPNPTSTGKNYSLIVAFNCIQKPSMKMAYVKFSSVFFMVITIAAYLWLP *F KFRSLHGKCCNLYFICLAITFLLNVISLFGIFELKTPICYLTGYAGYFTVMATFLWLSVISFDVWRRFAMRKFQVFYK *F NKRSSFFNYNIIVWSSAGLLTCIIFLVDQFVETNLDNPYNPAVGVFSCWIFTNGWSATFYFYAPLAILIILNCASFFL *F TTRYIYVENKQNQKVLNNSEPQKLSRNHANYRIYFRLFIIMGGSWFLEIIAFICEMENMWKPLIILNDYINCSQGIII *F FVATFCNHEMFRLIRKRIQNRNITSLELTNTSRPVESEKMADVELGK *F >mthl12 *F MFLWLKCFCTLIIVTIAKNSSAKIPHCKYDETINISHFKRLNDAYIYEHFEIPANLTGEFDYKELMDGSKVPTEFPNL *F RGCICKVRPCIRICCARKNILSNGECSDGVKNEIKLTMLDLTMQDILLTDPTLAELNMIPQYNSTELLILREQFQPCD *F EIVSLKRDEYTIFKDGSILLHTSAEILSNDQYCLYPEIYSDFPETIRIINRRCYRNVMPGIAQLSVISVVGFILTLAV *F YLSVEKLRNLLGKCLICSLFSMFMEYFIWTMDYFRLLQSICSAAGYMKYFFSMSSYLWFSVVSFHLWELFTSLNRHEP *F QYRFLIYNTFVWCTAAIPTVVIFSMNQMWENDPGKSEWLPLVGYFGCSVKDWNSSSWFYSHIPIVILNSFNVIMFVLT *F AIYIWKVKKGVKSFAQHDERNTTCLEFNVQTYIQFVRLFLIMGASWLLDQLTRLAEDSHLLLDTIVLNLTVYLNAAFG *F ILIFVLLILKGSTFKMIMERIRGTRFMTCLKGNQ *F >mthl13 *F MKLQLLGTFLVLILLQFQAVNTDSNKTEEDSCVKSDDDNSNDEDDLWHCIPICCPRKNMMANGGCSFKEHLFRTKLDL *F NIRLDDNSTEALYFNNQTLLITSFWDIDEMMGLRRDEYTLYKNGTIYIHSDQSIQTKEEYCFYPHQIYSDFPETIWII *F SHKFSSIESPGAFELASGSIICYIIIFGIYLFVKELRNDFGKCVMSCVFCLFLDYLIWLMDELRLLDDFCSLTGYIMF *F FFDFANSVWFSIISYCIWKKITSVVSQENRDQFVFYSTFAYGISAIPLGIIISINQFWEEDLRKWNWLPLVGFYLLQF *F SDCKRSSWVYYFVPYAIMCAINIIMFVLTIKHIMKTKRNLHNLTKRPDRNETCVTVNFLNFELYLLFLRISGIMGVAW *F ILIIFLLIEVNSTFWDIFGIIIQQIHYGFGIILFVLLIFKRSTHQLLIAKIRGKNLHQQTDV *F >mthl14 *F MNLGHWNFLLALISLQTFFNASAQISTVNNSSKGSNNSNIFHEDTFNSTSIDVLDASIHSTLVVPQTYSTEAATISGA *F RFATSVPVQSPVDNPLDPADCSQREKYRKQPVATPSSRLRKCCPHGENLNIYRENQSDSMCDNGLLSFEPTIISAVLF *F DNCIEDLEVETTLDYDIGNPCNSSLLYDDKDDVFFVLQDGSLLIIDKFGNESYTVKEHYCLDIDKSGHLFAFTCVTQV *F EEQIAFAKVVFVAVLMLISMPCLLLVSYLHMTLRLLRNLHGLSLSLMSLCLASGYFVHSVVHIYGIPNQGFIGYVIQF *F CILSYFFWYLCICFNVLLNVWYKLPCCIQCSKSWATFNFACYAVFAFSGPATIVALTVQKGLPGMPSYFLQGLTESIR *F DSQRYFIPPVSTILFLSFLLNIISFFGFQRISGYAKAEKNIQERKCLFDQQKYEDVKKDAKCVSLLGIIMVVSWLLEI *F ITFYSGSNSNYLILCDMVNGLQGVWVLLIFLVVRRRRTIILRWWYDRGSHEIEGTELQALSNSPT *F Hugh M. Robertson, Professor *F Department of Entomology, University of Illinois at Urbana-Champaign *F 320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801 *F Phone 217-333-0489; FAX 217-244-3499; email hughrobe@uiuc.edu *F WWW http://www.life.uiuc.edu/robertson/lab.html # *U FBrf0155353 *a Celniker *b S. *t 2002.7.30 *T personal communication to FlyBase *u FlyBase error report for CG1028 on Tue Jul 30 12:44:07 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 30 Jul 2002 12:44:07 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: celniker@bdgp.lbl.gov *F Subject: FlyBase error report for CG1028 on Tue Jul 30 12:44:07 2002 *F Error report from Susan Celniker (celniker@bdgp.lbl.gov) *F Gene or accession: CG1028 *F Release: 3 *F Missed gene *F Comments: This gene was correctly annotated in Release 2 please revert to the *F old annotation. It should have eight exons. .. # *U FBrf0155354 *a Bettencourt *b B. *t 2002.1.16 *T personal communication to FlyBase *u *F Date: Wed, 16 Jan 2002 12:26:57 \-0500 *F To: Yanmei Huang *F From: Brian Bettencourt *F Subject: Re: Structure of the 87C1 locus of D. melanogaster *F Hello! *F I have also been contacted by Wei Gong in K. Golic's lab regarding the 87C1 *F area and confusion over copy number. As far as I can tell, the y; cn bw sp *F chromosome which was sequenced in the Drosophila genome project has three, *F not two, tandemly repeated hsp70 genes at 87C1. If you look at BACR02G11, *F there \*is* a reverse-orientation hsp70 gene, hsp70Ba, near the beginning of *F the BAC. About 40kb downstream are the three tandemly-repeated *F copies. This arrangement, which I've never observed on any natural *F chromosomes in small-scale analyses, has been reported on the Cu Kar Sb *F balancer, and also in a Drosophila cell line. Detecting the 'extra' copy *F on natural chromosomes via Southern blotting or similar techniques would be *F very difficult given the extreme similarity of the two innermost tandem *F copies, *F and as such an exhaustive analysis has not been conducted. Thus, I can not *F speculate on the actual frequency of this gene arrangement in natural *F populations. *F If you can indeed verify that there the three hsp70s in tandem on *F BACR02G11, the problem will become one of nomenclature. The distalmost *F copy is definitely hsp70Bc, as it retains the divergent 3'-UTR. So what to *F call the other two tandem hsp70s, if the name hsp70Ba is reserved for the *F reverse-orientation copy? Given that the inner two tandem copies are *F virtually identical, I would propose hsp70Bb and hsp70Bb-prime or something *F similar. The original hsp70 nomenclature was proposed by Leigh Brown and *F Ish-Horowicz in 1981- *F Leigh Brown AJ, Ish-Horowicz D. *F Evolution of the 87A and 87C heat-shock loci in Drosophila. *F Nature. 1981 Apr 23;290(5808):677-82. # *U FBrf0155355 *a Tao *b Y. *t 2002.12.8 *T personal communication to FlyBase *u *F \-------------------------------------------------------------------------- *F To: gm119@gen.cam.ac.uk *F Subject: maur P's for curation *F From: Michael Ashburner (Genetics) *F Date: Mon, 16 Dec 2002 10:54:03 \+0000 *F Personal communication from Yun Tao , 8 Dec 2002. *F Dmau\P{lacW}* *F (True, Mercer & Laurie, 1996; FBrf0086665) *F line name insert cyt pos other info *F A1 15A *F D1 66D *F E1 9BC *F E4 67BC (I) *F F2, F3c 91A *F G2 35F *F I1 99D *F I2 27BC *F J1 62E *F L1b 61CD (I) *F L1c 3A (I) *F M2 50D *F O1 98DE lost (Oct'02) *F P1 72EF (I) lost (Oct'02) *F Q1 79F *F V1 42B *F W1 83AB *F X1 4F5A lost(1/15/99) *F Z1 10EF (I) *F 2A1 10EF (II) *F 2A2 9A (I) *F 2A3 46CD *F 2B1 71A (I) lost (Oct'02) *F 2E1 18DE (I) *F 2G3 12F (I) *F 2H1 45DE (I) *F 2H3 63C *F 2I2 52DE *F 2J1 45DE (II) *F 2K3 82A *F 2L1 90B Lost (9/00) *F 2M1 96E *F 2N1 26B Lost (9/00) *F 2O3 67BC (II) *F 2O5 93EF *F 2Q2 50CD *F 2R1 6EF (I) *F 2R2 84DE *F 2T1 99A (I) *F 2U1 3A (II) *F 2U2 71A (II) *F 2V1 24CD *F 2X2 6EF(II) *F 2Y1 99A (II) lost (Oct'02) *F 2Z1 35EF *F 3A1, 3A2 92E *F 3D2 72EF (II) *F 3E2 95A *F 3G1 7CD *F 3I1 92BC *F 3J1 78CD *F 3L1 18DE (II) *F 3N3 *F 3P1 64C *F 3Q1 35F36A *F 3Q3, 3Q3a 56DE (II) *F 3S1 6BC (I) *F 3T1 42E Lost (1/15/99) *F 3V1 19AB *F 3X1 19BC *F 3Y1 82C Lost (1/15/99) *F 3Z4 76C *F 4A1 97A *F 4B2 *F 4C1 85BC *F 4C2 61CD (II) *F 4D1 13A *F 4F1 69D *F 4F3 99A (III) Lost (9/00) *F 4G4C 34DE *F 4G5 33F34A *F 4H1 87EF *F 4I3 75C (II) *F 4J1 9A (II) *F 4K1 62BC *F 4L1 66EF *F 4M1 ? added to list(5/00) *F 4N1 65A *F 4N2 19EF *F 4O1 7A *F 4Q1 ? added to list(5/00) *F 4R1 4B (I) *F 4T1 29CD (I) Lost (9/00) *F 4V1 82E *F 4W1 86EF *F 4X1 47A *F 4Z2, 4Z3 71F72A *F AMY1 91BC *F BECKY1 4B (II) *F DEE1 57A *F EMMA1 65CD *F FAE1 67DE Lost (9/00) *F GINA1 58A *F GINA2 95EF *F ILEA1 12F (II) *F JOSIE2 13EF *F KIKI4 61CD (III) *F MAYBONNE1 9F *F NENEH2 1EF *F OPHELIA1 6BC (II) *F PANDORA1 13B Lost (9/01) *F QUEEN2 34AB Lost (9/01) *F RYLAH1 44D Lost (9/01) *F SHERI1 45DE (III) *F WANDA1 66B *F YAR1 86AB *F ZENA1 11C *F BART1 47F48A *F CALVIN2 29CD (II) *F ERNIE1 67F-68A *F ICHABOD1 24A *F \------------------------------------------------------------------------------ *F -- *F Date: Tue, 17 Dec 2002 18:43:11 \-0500 *F To: Gillian Millburn (Genetics) *F From: Yun Tao *F Subject: Re: FlyBase query *F Dear Gillian, *F Sorry for later reply. The numbers are corresponding like this: *F P44 92E 3A1 *F P42 91BC AMY1 *F P41 91A F2 *F P40 90B 2L1 (lost) *F P38 87EF 4H1 *F P37 86EF 4W1 *F P35 86AB YAR1 *F A full description for the introgression lines I made will soon be *F submitted to Genetics in the next one or two weeks. *F Thanks for curating this info, *F Yun *F >Dear Dr. Tao, *F > *F >I am a curator working for FlyBase. *F > *F >Michael Ashburner passed your information about the P{lacW} lines in D. *F >mauritiana to me so that I can record this information in the *F >database. *F > *F >I noticed that you also used some lines containing P{lacW} in D. *F >mauritiana in your paper: *F > *F >Tao et al., 2001, Proc. Natl. Acad. Sci. USA 98(23): 13183--13188 *F > *F >In that paper the lines are called: *F > *F >from Figure 2: P44, P42, P41, P40, P38, P37 and P35 *F > *F > *F >do any of these lines from Figure 2 correspond to the lines in the *F >information you sent Michael \- could you tell me which lines they *F >correspond to so that I can add that information into the database, *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F >-------------------------------------------------------------- *F > # *U FBrf0155358 *a Tricoire *b H. *t 2002.12.19 *T personal communication to FlyBase *u *F From tricoire@ijm.jussieu.fr Thu Dec 19 14:51:38 2002 *F To: ''Gillian Millburn \(Genetics\)'' *F Subject: RE : RE : UY530 gene *F Date: Thu, 19 Dec 2002 15:49:57 \+0100 *F Dear Gillian, *F The DIP3K2 gene correspond to the CG1630 gene. *F Best wishes *F Hervé *F \-----Message d'origine----- *F De : Gillian Millburn (Genetics) mailto:gm119@gen.cam.ac.uk *F Envoyé : jeudi 19 décembre 2002 15:36 *F À : tricoire@ijm.jussieu.fr *F Cc : gm119@gen.cam.ac.uk *F Objet : Re: RE : UY530 gene *F Dear Hervé, *F I have now curated both your papers on the 'UY530' insertion in the *F 'D-IP3K1' gene: *F Monnier et al., 2002, genesis 34(1-2): 76--79 *F Monnier et al., 2002, Free Rad. Biol. Med. 33(9) 1250--1259 *F so the information in them will make their way onto the public server in *F a few weeks, with the associated gene merge of 'UY530' and 'CG4026'. *F I have one question about your paper: *F Monnier et al., 2002, Free Rad. Biol. Med. 33(9) 1250--1259 *F In it you mention 'D-IP3K2' located on the X at 11F-12A. Could you tell *F me which predicted 'CG' gene 'D-IP3K2' corresponds to, so that I can *F rename it in FlyBase, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- # *U FBrf0155359 *a Hochheimer *b A. *t 2002.12.17 *T personal communication to FlyBase *u *F Date: Tue, 17 Dec 2002 11:59:18 \-0800 *F Subject: Hochheimer et al., 2002 *F From: Andreas Hochheimer *F To: gm119@gen.cam.ac.uk *F Hi Gillian, *F unfortunately we have missed this missmatch bug in the supplementary *F data. I am sorry for the confusion this might have caused. *F As you already expected, p67=p70=GH08269 cDNA *F and p63=p65=SD02767 cDNA *F Thanks for informing us. *F Best regards *F Andreas *F > To: jmlim@uclink4.berkeley.edu *F > Subject: FlyBase query *F > Cc: gm119@gen.cam.ac.uk *F > X-Sun-Charset: US-ASCII *F > From: Gillian Millburn (Genetics) *F > Date: Tue, 17 Dec 2002 12:59:24 \+0000 *F > *F > Dear Dr. Tjian, *F > *F > I am curating your paper for FlyBase: *F > *F > Hochheimer et al., 2002, Nature 420(6914): 439--445 *F > *F > and I have a question about two of the proteins in Figure 1 \- p67 and *F > p63. *F > *F > I looked in the Supplementary information for the paper and p67 and p63 *F > are not mentioned, but two proteins are mentioned in the supplementary *F > info which are not in Figure 1 \- these two proteins are p70 (EST *F > GH08269) and p65 (CG13176). *F > *F > Do p67 and p63 from Figure 1 of the paper correspond to p70 (EST *F > GH08269) and p65 (CG13176) respectively, or are they different ? *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- *F > *F \------------------------------------------------------------------------ *F \---------- *F Andreas Hochheimer *F University of California at Berkeley c/o Tjian *F Howard Hughes Medical Institute *F Department of Molecular and Cell Biology *F 401 Barker Hall \#3204 *F Berkeley, CA 94720-3204, USA *F (510) 643 3762 (lab) *F (510) 643 9547 (fax) *F hochheim@uclink4.berkeley.edu *F \------------------------------------------------------------------------ *F \----------- # *U FBrf0155360 *a Levis *b R. *t 2003.1.9 *T personal communication to FlyBase *u *F Date: Thu, 9 Jan 2003 19:21:56 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: Genes bft and MESK1 are probably allelic *F Cc: rolf@umich.edu, Gerry Rubin *F Hardiman et al. 2002 (FBrf0149016) have described the bereft (bft) *F gene and its association with transcripts that lack long ORFs. *F FlyBase has assigned this gene the synonym CR31863. In looking at *F this region with the Genome Browser, I noticed that EP(2)0980 is *F inserted near the 5' end of this gene. In fact, the insertion is *F located 13 nt upstream of the common 5' end of the long and short *F mRNAs of bft, as defined by the cDNAs reported by Hardiman et al. *F 2002 (accession AF486813 amd AF313801). Huang and Rubin 2000 *F (FBrf0131317) reported that EP(2)0980 interacts genetically with ksr *F and Ras85D. They named this gene Misexpression Suppressor of KDN 1, *F abbreviated as MESK1. They did not molecularly define the gene *F corresponding to MESK1. The proximity of EP(2)0980 to bft suggests *F that bft and MESK1 are allelic. *F As part of the BDGP Gene Disruption Project, we have localized *F several more P elements very close to the 5' end of bft. The line *F KG08390, which is now at the Bloomington stock center, is inserted 20 *F nt upstream of the 5' end of bft. A more recently-generated line, *F EY04690, is inserted in exon 1 of bft and is oriented such that it *F should permit Gal4-regulated mis-expression of the downstream *F sequences of bft. This line is currently being maintained in Hugo *F Bellen's lab, where it was generated, and can be requested through *F their P screen web site form *F (http://flypush.imgen.bcm.tmc.edu/pscreen/). Because of its *F proximity to KG08390, we will probably not send EY04690 to *F Bloomington unless it is sent as a replacement for KG08390. It would *F be interesting to see if either KG08390 or EY04690 show the *F interactions reported for mutant alleles of bft and MESK1. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0155379 *a Vanzo *b N. *t 2003.2.12 *T personal communication to FlyBase *u *F To: ephrussi@embl-heidelberg.de *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Wed, 5 Feb 2003 11:48:53 \+0000 *F Dear Dr. Ephrussi, *F I am curating your paper for FlyBase: *F Vanzo and Ephrussi, 2002, Development 129(15): 3705--3714 *F and I have a question about one of the constructs you used \- 'osk WT' *F in Figures 4 and 5. *F Is this wild-type osk construct the 6.45kb genomic rescue construct *F described in: *F Ephrussi and Lehmann, 1992, Nature 358(6385): 387--392 *F If it is not the 6.45kb rescue construct, please can you tell me a *F reference where 'osk WT' has been used, so that I can work out which *F construct in FlyBase it corresponds to, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F Date: Wed, 12 Feb 2003 19:06:57 \+0100 *F To: gm119@gen.cam.ac.uk *F From: Nathalie Vanzo *F Subject: Re: FlyBase query *F Dear Dr Millburn. *F Anne Ephrussi contacted me concerning your request on the construct we used *F \- 'osk WT' in Figures 4 and 5 of my paper. NO, this construct is not *F strictly identical to the wild-type osk 6.45kb genomic rescue construct *F described in: *F Ephrussi and Lehmann, 1992, Nature 358(6385): 387--392 *F The osk WT genomic construct I used is described in the Figure 3 of the *F same publication (Ephrussi and Lehmann, 1992, Nature 358(6385): 387--392) *F except that the bcd3'UTR was replaced by the osk3'UTR. This substitution *F was done as following: downstream of the stop codon TAA, I added a 1037bp *F WT genomic osk fragment beginning with the next osk codon and containing *F the full-length osk3'UTR. *F So basically the two constructs are different at the level of the osk *F promoter fragment which is slightly longer in the wild-type osk 6.45kb *F genomic rescue construct. *F Regards *F Nathalie *F ============================================ *F Dr. Nathalie Vanzo *F Ephrussi Group, Developmental Biology Programme *F European Molecular Biology Laboratory *F Meyerhofstrasse, 1 *F 69 117 Heidelberg *F Germany *F Tel: (49) 6221 387 616 *F Fax: (49) 6221 387 166 *F email : nathalie.vanzo@embl-heidelberg.de # *U FBrf0155381 *a Deal *b J. *c R. *d Andrade *e K. *f Cook *t 2002.12.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 17 00:33:04 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC27 *F Isolation and characterization of Df(3L)BSC27 *F Jennifer Deal, Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC27 was isolated as a P transposase-induced male recombination *F event involving P{lacW}l(3)L4060L4060 and P{PZ}corn04202. The deletion *F was isolated as a rho+-e+ recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{PZ}corn04202/P{lacW}l(3)L0460L0460 e1 males. Polytene chromosome *F squashes showed the breakpoints 65D4-5;65E4-6. Df(3L)BSC27 failed to *F complement sglA31, binI1 and msl-31. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155382 *a Deal-Herr *b M. *c K. *d Cook *t 2002.12.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 17 00:32:17 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2R)BSC26 *F Isolation and characterization of Df(2R)BSC26 *F Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2R)BSC26 was isolated as a P transposase-induced male recombination *F event involving P{lacW}corak08713 and P{PZ}hts01103. The deletion was *F isolated as a cn+-bw+recombinant chromosome from the cross cn1 bw1 *F females X cn1 P{PZ}hts01103/ P{lacW}corak08713 bw1 sp1; TMS, *F Sb1 P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 56C4;56D6-10. The deficiency chromosome retains the miniwhite *F marker from P{lacW}corak08713. Df(2R)BSC26 failed to complement wblE4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155383 *a Deal-Herr *b M. *c R. *d Andrade *e K. *f Cook *t 2002.12.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 17 00:31:24 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2R)BSC25 *F Isolation and characterization of Df(2R)BSC25 *F Megan Deal-Herr, Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2R)BSC25 was isolated as a P transposase-induced male recombination *F event involving P{lacW}walk14026 and P{PZ}Cct506444. The deletion was *F isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 *F females X cn1 P{PZ}Cct506444/P{lacW}walk14026 bw1 sp1; TMS, Sb1 *F P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints *F 48B7-C1;48C2-4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155384 *a Beckingham *b K. *t 2002.16.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Dec 16 19:34:33 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: UAS-Cam insertions *F The following information accompanied stocks donated by Kate Beckingham, *F Rice University (11/02). *F P{UAS-Cam.B34Q}3 *F P{UAS-Cam.V91G}3 *F P{UAS-Cam.B12Q}3 *F P{UAS-Cam.W}3 *F P{UAS-Cam.B1234Q}3 *F are all homozygous viable and fertile, third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155385 *a Roman *b G. *t 2002.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Dec 16 19:05:10 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Switch2}19-2 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Gregg Roman, Baylor College of Medicine (11/02). *F P{Switch2}19-2 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155386 *a Huet *b F. *c W. *d Gelbart *t 2002.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Dec 16 16:50:01 2002 *F To: flybase-updates@morgan.harvard.edu *F Subject: Huet et al. deletions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Francois Huet and Bill Gelbart, Harvard University (11/02). *F The following hobo-mediated deletions were isolated in Huet et al., 2002 *F (PNAS 99: 9948-9954) (FBrf0151478). The first two sets were described in *F detail. The third set was not described explicitly, but came from the same *F screen as the first set. *F 1. Deletions isolated as w+ y- progeny following hobo mobilization *F from P{wHy}01D01. All retain a P{w+mC=5'wHy}. The numbers give the size *F of the deletion in base pairs. The Roman numerals refer to the deletion *F class in Figure 3B. GenBank accession numbers for the sequences of *F deletion endpoints are given in Huet et al. *F Df(2R)01D01W-L133 46568 II *F Df(2R)01D01W-L186 105231 III *F Df(2R)01D01W-L197 204791 III *F Df(2R)01D01W-L053 320378 IV *F Df(2R)01D01W-L149 398322 IV *F 2. Deletions isolated as w- y+ progeny following hobo mobilization *F from P{wHy}01D09. All retain a P{y+t7.7=3'wHy}. The numbers give the *F size of the deletion in base pairs. The Roman numerals refer to the *F deletion class in Figure 3A. GenBank accession numbers for the sequences *F of deletion endpoints are given in Huet et al. *F Df(2R)01D09Y-M088 78060 I *F Df(2R)01D09Y-M125 101979 II *F Df(2R)01D09Y-M186 200920 III *F Df(2R)01D09Y-M092 329337 IV *F Df(2R)01D09Y-M073 353318 IV *F 3. Deletions isolated as w- y+ progeny following hobo mobilization *F from P{wHy}01D01. All retain a P{y+t7.7=3'wHy}. The numbers give the *F size of the deletion in base pairs. *F Df(2R)01D01Y-R11 50175 *F Df(2R)01D01Y-R09 103508 *F Df(2R)01D01Y-R24 240928 *F Df(2R)01D01Y-R23 361566 *F Df(2R)01D01Y-R21 492864 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155387 *a Cherbas *b L. *t 2002.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Dec 13 17:22:45 2002 *F To: rd120@gen.cam.ac.uk *F Subject: Cherbas constructs *F Cc: lcherbas@sunflower.bio.indiana.edu *F The following information was provided to the Bloomington Stock Center by *F Lucy Cherbas, Indiana University (12/02). *F P{UAS-EcR.C}TP1-4 and P{Sgs3-GAL4.PD}TP1 are homozygous viable and fertile, *F third chromosome insertions. *F P{UAS-EcR.B1-DeltaC655.F645A}TP1, P{Eip71CD-GAL4.PC}TP1-1 and *F P{UAS-EcR.B1-DeltaC655.W650A}TP1-9 are homozygous viable and fertile, *F second chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155388 *a Levis *b R. *t 2002.11.27 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Wed Nov 27 20:41:48 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: CG14750 and l(2)44Db should be merged *F There is evidence that the genes CG14750 and l(2)44Db should be *F merged. Dockendorff et al.2000 FBrf0126727 determined that the *F mutant l(2)k08904 is an allele of a complementation group they called *F l(2)44Db. There is a P-element insertion in l(2)k08904 that is *F within the protein coding sequences of the gene CG14750 (at *F AE003836.4 nucleotide 91180). The estimated cytogenetic location of *F CG14750 (2R-44D5) is consistent with it being l(2)44Db. Neither of *F these two genes currently lists the other as a synonym. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0155389 *a Levis *b R. *t 2002.10.23 *T personal communication to FlyBase *u *F To: flybase-help@morgan.harvard.edu *F Date: Wed, 23 Oct 2002 15:12:22 \-0400 *F From: Robert Levis *F Subject: BEST:HL05962 and CG8788 are synonyms *F I think that the genes BEST:HL05962 and CG8788 are synonyms and *F should be merged. The only mutant allele for BEST:HL05962 is *F BEST:HL05962k04512. A stock carrying this allele is available from *F the Bloomington stock center. This allele is associated with a *F P-element insertion in a small intron of the gene CG8788. The *F FlyBase reports for these two genes do not reference each other. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0155390 *a Levis *b R. *t 2002.11.5 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Tue Nov 05 22:49:19 2002 *F To: flybase-help@morgan.harvard.edu *F Subject: P{lacW} n(2)k04810 is an allele of CG6426, not Ef1beta *F P{lacW}n(2)k04810 is currently recorded as an allele of the Ef1beta *F gene. I did a BLAST of the flanking sequence for P{lacW} n(2)k04810 *F (accession AQ025749) vs. WGS release 3 and localized the insertion to *F scaffold segment AE003805.3 position 137074. This is 265 bp 3' of *F the Ef1beta gene and is actually at \+9 bp of the CG6426 gene. *F Therefore, I suggest making this insertion an allele of the CG6426 *F gene rather than the Ef1beta gene. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0155392 *a Britt *b S.G. *t 2002 *T personal communication to FlyBase *u *F > From steve.britt@uchsc.edu Mon Nov 18 22:09:09 2002 *F > To: flybase-help@morgan.harvard.edu *F > Subject: rh6 gene *F > *F > I am writing to inquire about sending an update concerning one of the *F > genes in flybase (Rh6), for which we identified a mutant allele *F > several years ago (unpublished). The mutant allele Rh61 is *F > identical to that found in the published genome sequence of this *F > gene, and we have found that the strain the genome sequence was *F > obtained from (y1; cn1, bw1, sp1 Bloomington stock number *F > 2057) is phenotypically mutant for Rh6. I wanted to find out who to *F > contact so that the rh6 entries in flybase, gadfly and the stock *F > center can be amended. *F > *F > Thanks, *F > *F > Steve *F > \-- *F > _______________________________________ *F > *F > Steven G. Britt, M.D. *F > Associate Professor *F > Department of Cellular and Structural Biology *F > Department of Ophthalmology, SOM Room 4547 *F > University of Colorado Health Sciences Center *F > 4200 East 9th Ave., Campus Box B111 *F > Denver, CO 80262 *F > *F > phone: 303-315-0880 *F > fax: 303-315-4729 *F > lab: 303-315-0912 or 315-6282 *F > dept. phone: 303-315-7009 *F > *F > email: steve.britt@uchsc.edu # *U FBrf0155393 *a Cooley *b L. *t 2002.12.31 *T personal communication to FlyBase *u *F > On 7/17/02 10:50 AM, 'Rachel Drysdale (Genetics)' *F > wrote: *F > *F > Hi Lynn, *F > *F > I'm curating this paper of your for FlyBase: *F > \*x FBrf0147062 == Buszczak et al., 2002, Genetics 160(4): 1511--1518 *F > *F > I have a question about 'es(3)79' (M and Ms, Drosophila stocks section, *F > last sentence). Is it by any chance *F > FBti0003868 == P{A92}M34a *F > ? *F > I wouldn't want to make a new insertion record for it if we already *F > have it in FlyBase, so I thought I would check. *F > *F > If it isn't P{A92}M34a, then please let me know which element is *F > inserted and its site of location in the genome and I'll have another *F > go at tracking it down. *F > *F > Thanks for your help. *F > Best wishes, *F > *F > Rachel. *F > \---------------------------------------------------------------------- *F > Rachel Drysdale, Ph.D. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: rd120@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > *F > FlyBase: http://fly.ebi.ac.uk:7081/ *F > \---------------------------------------------------------------------- *F > From lynn.cooley@yale.edu Tue Dec 31 15:30:06 2002 *F > Subject: Re: Helping FlyBase: es(3)79 *F > To: 'Rachel Drysdale (Genetics)' *F > *F > Dear Rachel, *F > *F > As part of getting ready for the new year, I have been cleaning out my in *F > box and found this message. This is an enhancer trap element generated *F > years ago in Allan Spradling's lab. I have never mapped the insertion site, *F > and I don't know if corresponds to the entry you mentioned. *F > *F > *F > Happy new year, *F > Lynn *F > *F > *F > On 1/2/03 11:31 AM, 'Rachel Drysdale (Genetics)' *F >wrote: *F > Hi Lynn, *F > *F > Happy New Year! *F > *F > Thanks for this reply. Do *F > you recall which enhancer trap element (e.g. P{lArB} vs P{lacW} or *F > whatever)? If not I'll simply record it as P{lacZ}es79 (and that it is *F > on the third which is what I presume the (3) denotes), but it would be *F > nice to include the element specifics if possible. *F > *F > All the best *F > *F > Rachel. *F > *F > *F >From lynn.cooley@yale.edu Thu Jan 02 16:59:44 2003 *F >Subject: Re: Helping FlyBase: es(3)79 *F >To: 'Rachel Drysdale (Genetics)' *F > *F >Rachel, *F > *F >I am pretty sure it was P{lacW}, and its definitely on the third. *F > *F >Regards, *F >Lynn *F > *F >-- *F >Lynn Cooley, Ph.D. *F >Departments of Genetics and Cell Biology *F >Yale University School of Medicine *F >333 Cedar St., PO Box 208005 *F >New Haven, CT 06520-8005 *F > *F >203-785-5067 voice *F >203-785-6333 fax *F >lynn.cooley@yale.edu *F >http://info.med.yale.edu/cooley # *U FBrf0155394 *a Roote *b J. *t 2003.1.8 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Wed Jan 08 18:37:11 2003 *F To: prh@bcm.tmc.edu *F Subject: Re: P-Screen Project Update November 2002 *F Cc: Rachel Drysdale *F Dear Robin, *F I don't know if you collect genetic information on your P inserts. *F But just in case you do (and copied to FlyBase): *F BG00682 is a viable ck allele. It has a strong ck phenotype over ck deletions. *F BG02008 is a male-sterile allele of l(2)35Di (BG02008 is inserted in *F BG:DS09217.1. i.e. this result identifies l(2)35Di). *F KG00239 is a lethal allele of CycE. *F Regards, *F John Roote. *F >Dear Drosophilist, *F > *F >the database of the ongoing P-Screen / Gene disruption project of the *F >Bellen/Rubin/Spradling labs has been updated today and is available at: *F > *F >http://flypush.imgen.bcm.tmc.edu/pscreen *F > *F >A total of 4357 (+194 additional temporary lines) have so far been made *F >available, 2570 of which are already available from the Bloomington *F >Stockcenter. Please note that numerous lines that were available in the *F >past have been replaced in the meantime. *F > *F >--- *F > *F > P. Robin Hiesinger, Ph.D. Bellen Lab *F > Howard Hughes Medical Institute Baylor College of Medicine *F > One Baylor Plaza Houston, Texas 77030 *F > Rm. T630, Mail Stop BCM235 Tel: 713 798 6928 Fax: 713 798 3694 *F \-- *F ___________________________________________________________________ *F John Roote *F Ashburner Lab *F Department of Genetics *F University of Cambridge *F Downing Street *F Cambridge *F CB2 3EH *F UK *F Office: \+44 1223 765124 *F Lab: \+44 1223 333982 *F Fax: \+44 1223 333992 *F email: jr32@mole.bio.cam.ac.uk *F website: http://www.gen.cam.ac.uk/dept/ashburner.html # *U FBrf0155395 *a Postigo *b A. *t 2002.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 07 15:20:44 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Postigo constructs and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Antonio Postigo, Washington University in St. Louis (12/02). *F P{UAS-zfh1} is the full length zfh1 coding sequence inserted in the *F NotI/XbaI cloning site of P{UAST}. *F P{UAS-zfh1-DeltaHD} is identical to P{UAS-zfh1} except the homeodomain *F (corresponding to nucleotides 2110 to 2293: CGGAAAGTCCG... to *F ....CGCAAAATGCAG) was replaced by a KpnI site. *F P{UAS-zfh1-CIDm} is identical to P{UAS-zfh1} except the CtBP interacting *F domain (PLDLS at nucleotides 2374 to 2388: CGCTGGACTTGTCC) was changed to *F ASASA. A description of the CtBP interacting domain is given in Postigo *F and Dean, 1999 (PNAS 96: 6683-6688; FBrf0111586). *F P{UAS-zfh1}2B, P{UAS-zfh1-CIDm}2B and P{UAS-zfh1-DeltaHD}2B are homozygous *F viable and fertile, second chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155396 *a Cook *b K. *t 2003.1.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 20 21:48:11 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Revised cytology of Df(2R)vg135 *F Polytene squashes of the deficient inversion Df(2R)vg135 showed that 49B7, *F 49E1,2 and all the bands between are deleted, while 49B1,2 and 49F1,2 are *F still present. The inversion breakpoint falls between 47F16 and *F 47F18. Therefore, the cytology of the aberration is 49B2-7;49E2-F1;47F16-18. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155397 *a Roote *b J. *t 2002.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 21 17:20:14 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Cambridge insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote, Cambridge U. (12/02). *F P{70FLP}7 is an insertion on the SM6b balancer. (We will list the balancer *F variant as SM6b, P{ry+t7.2=70FLP}7 in our stocklist.) *F P{RS3}CB-0100-3 is an insertion on the SM6a balancer. (We will list the *F balancer as SM6a, P{w+mW.Scer\FRT.hs=RS3}CB-0100-3 in our stocklist.) *F P{RS3}l(1)CB-6411-31 is a lethal insertion on the X chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155398 *a Deal *b J. *c K. *d Cook *t 2003.1.30 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jan 30 20:14:07 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC28 *F Isolation and characterization of Df(2L)BSC28 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Df(2L)BSC28 was isolated as a P transposase-induced male recombination *F event involving P{PZ}toc01361 and P{EP}EP2297. The deletion was isolated *F as a net+-cn+ recombinant from the cross net1 b1 cn1 females x *F P{PZ}toc01361 cn1/net1 P{EP}EP2297; TMS, Delta2-3 males. Polytene *F chromosome squashes showed the breakpoints 23C5-D1;23E2. Df(2L)BSC28 failed *F to complement Madk00237, l(2)23DbA18-1 and l(2)23DaA5-2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155399 *a Cook *b K. *t 2003.2.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jan 31 15:13:43 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: RF32 *F Hi folks-- *F I'd like to propose merging two entries: tsg (FBgn0003865) and l(1)11Ac *F (FBgn0001519). *F George Lefevre isolated a mutation called RF32, which is listed as both *F l(1)11Ac3 and tsg4. The l(1)11Ac entry cites Schalet, 1986 *F (FBrf0045066) and Abe said he got the mutation from Lefevre. The tsg4 *F entry cites Perrimon et al., 1989 (FBrf0049894) and they say they got the *F mutation from Lefevre, too. *F It's probably best to retain the tsg4 name. *F Thanks! *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155400 *a Carpenter *b A.T.C. *t 2003.1.31 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jan 31 20:31:39 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df information *F The following information about deletions in the Bloomington collection was *F provided by Adelaide Carpenter, Cambridge University (1/03). *F Revised cytological breakpoints: *F Df(3R)DG2 89E9;91A3-7 *F Df(3R)RD31 89E2-3;90D2-4 *F Df(3R)P14 90C7+;91B1-2 *F Df(3R)Cha1a 91A8-B1;91F12-92A1 *F Df(3R)sr16 90C7-8;90E1-2 *F Df(3R)DG4 90D2+;90F6-8 *F The following combinations are lethal: *F Df(3R)p14/Df(3R)Cha7 *F Df(3R)P14/Df(3R)Cha1a *F Df(3R)DG2/Df(3R)Cha7 *F Df(3R)RD31/Df(3R)sr16 *F Df(3R)RD31/Df(3R)DG4 *F Df(3R)DG4/Df(3R)Cha7 *F The following combinations are viable and female fertile: *F Df(3R)sr16/Df(3R)Cha7 *F Df(3R)sr16/Df(3R)Cha1a *F Df(3R)DG4/Df(3R)Cha1a *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0155401 *a Malik *b H.S. *t 2003.2.8 *T personal communication to FlyBase *u *F From hsmalik@fhcrc.org Sat Feb 08 01:51:13 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: CG4715 nomenclature *F Cc: steveh@fhcrc.org *F Dear Flybase colleagues, *F We would like to ascribe the name 'Iris' to CG4715 in flybase. We *F have an abstract in the forthcoming Drosophila Research Conference *F (below) in which we have carried out an extensive evolutionary *F analysis of CG4715 and shown its evolutionary relatednes to the *F envelope genes from two classes of insect viruses: the baculoviridae *F and the errantiviridae. *F We have named this gene Iris because it is a host gene (not a dead *F virus) and plays an important role in host oogenesis, but is related *F to 'pathogenic' viruses. *F IRIS, in Greek mythology, Goddess of the Rainbow, the daughter of the *F Titan Thaumas and Electra, daughter of the Titan Oceanus. As *F messenger of the god Zeus and his wife, Hera, Although she was a *F sister of the winged monsters, the Harpies, Iris was represented as a *F beautiful maiden, with wings and robes of bright colors and a halo of *F light on her head, trailing across the sky with a rainbow in her wake. *F We have a manuscript in preparation which we would also like to use *F this nomenclature. Please let me know if this is okay and what *F procedures we should follow. *F (poster) *F Program Nr: 926B *F Iris, a viral envelope related host gene in Drosophila. H.S. Malik , *F S. Henikoff. *F Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA. *F Mobile genetic elements impose a high cost on the host genomes they *F infest. Yet, occasionally their enzymatic machinery gets usurped for *F beneficial function to eukaryotic genomes, from protecting the ends *F of linear chromosomes to generating diversity in immune function. In *F rare cases, structural genes of mobile elements have been acquired by *F host genomes. Here we report on a retroviral envelope-related gene, *F Iris, that has been preserved solely as a host gene (not in the *F context of a virus or provirus) in Diptera. Cytological analyses *F indicate that Iris plays an important role in oocyte development in *F D. melanogaster, while molecular evolutionary studies suggest that *F dual selective pressures *F act on the gene. By analogy to the gypsy-like envelope genes, the *F receptor-interacting segment of Iris is under strong positive *F selection, while the structurally important membrane-fusion segment *F is under balancing selection in D. melanogaster. Iris provides a *F unique opportunity to study the process of 'domestication' of a *F selfish gene. *F (end) *F You may address any queries to me or to Steve Henikoff *F (steveh@fhcrc.org) who is the senior author on this study. *F Best wishes, *F Harmit Malik *F \-- *F Dr. Harmit Singh Malik *F Henikoff Lab, Fred Hutchinson Cancer Research Center, *F 1100 Fairview Ave. N, A1-162, Seattle, WA 98109-1024. *F Tel. (206)-667-4509; Fax. (206)-667-5889 *F http://blocks.fhcrc.org/~harmit # *U FBrf0155402 *a Levis *b R. *t 2003.3.6 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Thu Feb 06 20:20:13 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: P{hsneo}l(3)neo381 is inserted in the CG31364 gene *F Based on the flanking sequence, GenBank AQ034103, the insertion in *F P{hsneo}l(3)neo381 is within the protein coding sequence of the *F CG31364 gene. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0155403 *a White-Cooper *b H. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:19:50 2003 *F To: helen.white-cooper@zoo.ox.ac.uk *F Subject: Helping FlyBase: ADRC-10302 *F Dear Helen, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The aly \-class meiotic arrest genes of Drosophila: transcriptional regulators *F in primary spermatocytes. *F You mention a gene symbol that is new to FlyBase, comr. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From helen.white-cooper@zoology.oxford.ac.uk Mon Feb 10 10:30:36 2003 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 10 Feb 2003 10:30:36 \+0000 *F Mime-Version: 1.0 *F X-Sender: zool0195@zool0195.herald.ox.ac.uk (Unverified) *F Date: Mon, 10 Feb 2003 10:30:35 \+0000 *F To: Rachel Drysdale (Genetics) *F From: Helen White-Cooper *F Subject: Re: Helping FlyBase: ADRC-10302 *F comr is an abbreviation for cookie monster. It is the final name for *F the gene we had been calling zab. it is CG13493. The paper *F describing the cloning etc is coming out in development this month. *F helen *F \-- *F \------------------------------------------------------------------------------ *F ------------------------------------------------------------------------------- *F --- *F Helen White-Cooper *F Dept of Zoology *F University of Oxford *F South Parks Road *F Oxford *F OX1 3PS *F Tel: \+44 (0)1865 271195 (office) 271291 (lab) *F Fax: \+44 (0)1865 310447 # *U FBrf0155404 *a Kuranaga *b E. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:12:58 2003 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 10 Feb 2003 10:12:58 \+0000 *F To: kuranaga@brain.riken.go.jp *F Subject: Helping FlyBase: ADRC-10119 *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 10 Feb 2003 10:13:00 \+0000 *F Content-Length: 1075 *F Dear Erina, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Degradation of IAP protein induces DTRAF1-dependent JNK activation. *F You mention a gene symbol that is new to FlyBase, Ask1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kuranaga@brain.riken.go.jp Mon Feb 10 10:35:25 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10119 *F Dear Dr. Rachel Drysdale, *F Thank you for your kind asking. The Genome Project CG annotation no. *F of DASK1 is CG4720. This gene was reported as PK92B, but the reported *F PK92B cDNA had a 1-bp deletion and a 1-bp insertion at nucleotide, and *F the resulting clone contained the longer ORF. So, we re-named this gene *F as DASK. Could you use CG4720 for Flybase? *F Best regards, *F Erina Kuranaga # *U FBrf0155405 *a Orihara *b M. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:07:07 2003 *F To: orihara@cdb.riken.go.jp *F Subject: Helping FlyBase: ADRC-10079 *F Dear Minako, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Analysis of a Drosophila gene, slender lobes, encoding a novel factor *F involved in the brain development. *F You mention a gene symbol that is new to FlyBase, sle. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From orihara@cdb.riken.go.jp Mon Feb 10 11:05:43 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10079 *F Dear Rachel, *F Our data show that sle corresponds to CG12819 on 3R, 86A8. *F Thank you for all your efforts. *F Minako. # *U FBrf0155406 *a Daga *b A. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:20:15 2003 *F To: daga@unipd.it *F Subject: Helping FlyBase: ADRC-10306 *F Dear Genny, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Functional analysis of the Drosophila spastin gene. *F You mention a gene symbol that is new to FlyBase, Spastin. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Also, if you have settled on a short symbol for your gene now would be *F a good time to let me know what it is, as then I can enter it in the *F database from the outset. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From daga@unipd.it Mon Feb 10 11:41:19 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Helping FlyBase: ADRC-10306 *F Dear Rachel, *F the gene mentioned in the abstract is the homolog of the human SPG3A *F (spastin) gene. It corresponds to CG5977, and we call it D-spastin. I would *F suggest spas as a shorthand, but feel free to change to whatever you like *F in case the symbol already existed. *F If you need further clarifications, please let me know. *F Regards, *F andrea *F Andrea Daga *F Assistant Telethon Scientist *F Dulbecco Telethon Institute and *F Department of Pharmacology *F University of Padova *F Largo Meneghetti 2 *F 35121 Padova \- Italy *F Tel: \+39-0498275778 *F Fax: \+39-0498275093 *F email: daga@unipd.it adaga@dti.telethon.it # *U FBrf0155407 *a Rasheva *b V.I. *t 2003.2.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:25:39 2003 *F To: vauya@imb.sinica.edu.tw *F Subject: Helping FlyBase: ADRC-10257 *F Dear Vanya, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Overexpression of dMBD-R2 Causes Changes in Drosophila Development. *F You mention a gene symbol that is new to FlyBase, MBD-R2. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From vauya@imb.sinica.edu.tw Mon Feb 10 11:41:26 2003 *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10257 *F Dear Rachel, *F dMBD-R2 is synonym of the CG10042. *F Best wishes: *F Vanya # *U FBrf0155408 *a Kassis *b J. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:23:29 2003 *F To: jk14p@nih.gov *F Subject: Helping FlyBase: ADRC-10428 *F Dear Judith, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Drosophila pho-like gene encodes a YY1-related DNA binding protein that is *F redundant with pleiohomeotic in homeotic gene silencing. *F You mention a gene symbol that is new to FlyBase, pho-like. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jkassis@mail.nih.gov Mon Feb 10 14:04:48 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10428 *F Dear Rachel, *F pho-like is CG3445. The symbol is phol. We have a paper on it in *F January Development. *F Best wishes, *F Judy # *U FBrf0155409 *a Norga *b K.K. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 09:51:24 2003 *F To: knorga@bcm.tmc.edu *F Subject: Helping FlyBase: ADRC-10024 *F Dear Koenraad, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Lambik, a novel putative LRR/Immunoglobulin transmembrane protein, is a *F bristle number QTL that modulates Notch signaling. *F You mention a gene symbol that is new to FlyBase, lbk. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From knorga@bcm.tmc.edu Mon Feb 10 14:05:36 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10024 *F Cc: hbellen@bcm.tmc.edu *F Dear Rachel, *F Thanks for your inquiry. To the best of our understanding lbk corresponds *F to CG8434. *F Koenraad # *U FBrf0155410 *a Orian *b A. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:00:38 2003 *F To: aoryan@fhcrc.org *F Subject: Helping FlyBase: ADRC-10766 *F Dear Amir, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Global analysis of genome binding by the Drosophila Myc, Max, Mad/Mnt *F transcription factor network. *F It seems likely that the Mad that you are writing about is Mad-3E *F (FBgn0024333) as opposed to Mad:Mothers against dpp (FBgn0011648). *F Could you confirm this for us please? Also, your abstract seems to *F suggest that Mad-3E and Mnt (FBgn0023215) are the same gene. Again if *F you could confirm this for us that would be great. We will merge the *F gene records in FlyBase, if so. We would prefer to go with the Mnt *F gene symbol, to reduce to possibility of confusion with FBgn0011648, *F and hope that you would use Mnt in future publications. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From aoryan@fhcrc.org Mon Feb 10 14:14:56 2003 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10766 *F Dear Rachel, *F thank you for your care. *F Yes, you are correct Mad-3E is dMnt. ( dMax heterodimer partner) *F I agree that the Mnt symbol will be better as to not confuse the reader with *F dpp related Mad *F (FBgn0011648) *F In the future and in an upcoming publication we are already using the term *F dMnt. *F Best *F Amir *F Dr. Amir Orian (Oryan) MD/PhD *F Postdoctoral fellow *F Robert Eisenman Lab *F Division of Basic Sciences *F Fred Hutchinson Cancer Ctr. *F 1100 Fairview Ave. N Seattle WA 98109 *F 206-667-4446 Fax: 206-667-6522 aoryan@fhcrc.org # *U FBrf0155411 *a Curtiss *b J. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:04:23 2003 *F To: jennifer.curtiss@mssm.edu *F Subject: Helping FlyBase: ADRC-10895 *F Dear Jennifer, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F dan and danr encode novel factors involved in specification of both eye and *F antennal development. *F You mention genes that are new to FlyBase, dan and danr. Do you know *F which of the Genome Project CG annotations your genes correspond to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Jennifer.Curtiss@mssm.edu Mon Feb 10 14:17:09 2003 *F Subject: Re: Helping FlyBase: ADRC-10895 *F To: Rachel Drysdale (Genetics) *F dan is CG11849, danr is CG13651 # *U FBrf0155412 *a Ivanovska *b I. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 09:57:42 2003 *F To: ivanovska@wi.mit.edu *F Subject: Helping FlyBase: ADRC-10621 *F Dear Irena, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Role of MOS in the meiotic cell cycle in Drosophila. *F You mention a gene symbol that is new to FlyBase, mos. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F Is it by any chance CG8767 (FBgn0033773)? All the CGs have *F corresponding gene records in FlyBase already and we don't like to make *F duplicate records for what is actually the same gene unless we can't *F avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From ivanovska@wi.mit.edu Mon Feb 10 15:15:32 2003 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10621 *F Hi Rachel, *F The mos I refer to is indeed CG8767. Hope this helps. *F Irena # *U FBrf0155413 *a Bejsovec *b A. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:22:04 2003 *F To: wmj2@duke.edu *F Subject: Helping FlyBase: ADRC-10378 *F Dear Whitney, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of a mutation that modulates the wingless mutant phenotype. *F You mention a gene symbol that is new to FlyBase, eon. Do you now know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From bejsovec@duke.edu Mon Feb 10 15:18:36 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: 10378 \- eon *F Hi Rachel, *F You don't need to worry about curating eon \-- we've figured *F out that the mutations are in RacGap50C, formerly CG13345. Now we *F just have to figure out why it's modulating the wg phenotype! *F Take care, *F Amy *F \-- *F Amy Bejsovec, Ph.D. *F Duke University *F Dept. of Biology/DCMB Group *F Box 91000 *F B336 LSRC, Research Dr. *F Durham, NC 27708-1000 *F (919) 613-8162 office email: bejsovec@duke.edu *F (919) 613-8163 lab FAX: (919) 613-8177 *F (919) 613-8153 fly room # *U FBrf0155414 *a Jemc *b J. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:16:42 2003 *F To: jcj27@mit.edu *F Subject: Helping FlyBase: ADRC-10244 *F Dear Jennifer, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of the spen paralog spenito during embryonic and retinal *F development. *F You mention a gene symbol that is new to FlyBase, nito. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From jcj27@MIT.EDU Mon Feb 10 15:20:09 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10244 *F The CG number that corresponds to nito is CG2910. *F Thanks, *F Jennifer # *U FBrf0155415 *a Goode *b S. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:12:15 2003 *F To: sgoode@bcm.tmc.edu *F Subject: Helping FlyBase: ADRC-11010 *F Dear Scott, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Opposing Regulation of Cell Cluster Movement by Fasciclin II via Neoplastic *F Tumor Suppressors and a Drosophila AIB1 Negative Feedback Loop. *F You mention a gene symbol that is new to FlyBase, Aib1:Amplified in *F breast cancer-1. Do you know which of the Genome Project CG *F annotations your gene corresponds to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From sgoode@bcm.tmc.edu Mon Feb 10 15:34:26 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-11010 *F The gene is also called Taiman, discovered in Denise Montell's lab. *F At a recent cell migration meeting her and members of her lab *F also frequently referred to it as DAIB1. *F Scott # *U FBrf0155416 *a Qian *b L. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:18:11 2003 *F To: qianl@umich.edu *F Subject: Helping FlyBase: ADRC-10256 *F Dear Li, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The function of neuromancer, the invertebrate orthologue of human TBX20, in *F heart, muscle and CNS development. *F I'm writing about the nmr:neuromancer gene (which you also refer to as *F H15). FlyBase already has a gene record for H15 (FBgn0016660). Is *F this the same gene as neuromancer? *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From qianl@umich.edu Mon Feb 10 16:03:24 2003 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10256 *F Dear Rachel *F Yes, neuromancer is H15. Since we found interesting potential *F function of *F H15 in both CNS and heart, we rename it as neuromancer. *F Any questions, just feel free to contact me. *F Have a nice day! *F Li # *U FBrf0155417 *a Ketel *b C. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:00:12 2003 *F To: kete0013@umn.edu *F Subject: Helping FlyBase: ADRC-10759 *F Dear Carrie, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Analysis of Histone Methyltransferases in Drosophila . *F You mention a gene symbol that is new to FlyBase, Set8. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kete0013@umn.edu Mon Feb 10 16:09:21 2003 *F Subject: Re: Helping FlyBase: ADRC-10759 *F To: Rachel Drysdale *F Rachel, *F Set8 corresponds to CG3307. The reference is Fang et al, Current Biology, *F 2002, Vol 12, 1086-1099. If you have any other questions, please let me *F know. *F Thanks, *F Carrie # *U FBrf0155418 *a Xu *b H. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:13:37 2003 *F To: xuhong@jhmi.edu *F Subject: Helping FlyBase: ADRC-10156 *F Dear Hong, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F You mention a gene symbol that is new to FlyBase, sunglass. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F Also, if you have settled on a short symbol for your gene now would be *F a good time to let me know what it is, as then I can enter it in the *F database from the outset. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From XUHONG@JHMI.EDU Mon Feb 10 16:24:46 2003 *F Subject: Re: Helping FlyBase: ADRC-10156 *F To: Rachel Drysdale (Genetics) *F Hi, Rachel: *F The flybase annotation for sunglass:CG12143, which map to the 2-42E, encodes *F a ttraspanin protein. *F . *F . *F thanks *F Hong Xu # *U FBrf0155419 *a Chang *b H. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 09:50:14 2003 *F To: henry@chronos.med.yale.edu *F Subject: Helping FlyBase: ADRC-10015 *F Dear Henry, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Roles of Hsc70 and Rme-8 in Clathrin-mediated Endocytosis. *F You mention a gene symbol that is new to FlyBase, Rme-8. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your gene does not correspond to a CG *F then perhaps you could tell me its genomic location, as this is *F valuable information for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From henry@chronos.med.yale.edu Mon Feb 10 16:35:08 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10015 *F Hi Rachel, *F Rme-8 is CG8014 at 45B3. I kept this name from the homolog first *F identified in C.elegans. Rme stands for Receptor Mediated Endocytosis *F which is quite appropriate for the description of its function. As *F far as allele goes, it is allelic with l(2)45Ba. Although this is *F information is not yet published, it should be out soon because I am *F currently preparing a manuscript on this. *F Hope this helps. *F \-H *F \-- *F Henry Chang *F Department of Cell Biology *F Yale University School of Medicine *F SHM C435 *F 333 Cedar St. *F New Haven, CT 06510 *F 203-785-6078 *F From henry@chronos.med.yale.edu Wed Feb 12 21:47:13 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10015 *F Hi Rachel, *F The mutation in l(2)45Ba is single nucleotide (G to A) substitution *F at amino acid position 1287 (normally a tryptophan, W). This mutation *F generates a premature stop codon at that amino acid position. *F \-H *F From rd120@gen.cam.ac.uk Wed Feb 12 21:54:29 2003 *F To: henry@chronos.med.yale.edu *F Subject: Re: Helping FlyBase: ADRC-10015 *F Hi Henry, *F thanks for this ... *F >The mutation in l(2)45Ba is single nucleotide (G to A) substitution *F >at amino acid position 1287 (normally a tryptophan, W). This mutation *F >generates a premature stop codon at that amino acid position. *F I'm not quite clear which allele this is ... *F thanks *F Rachel. *F From henry@chronos.med.yale.edu Thu Feb 13 15:42:33 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10015 *F Hi Rachel, *F l(2)45Ba3. The mutant available from Bloomington. I generated other *F alleles as well but they haven't been sequenced yet. *F \-H *F \-- *F Henry Chang *F Department of Cell Biology *F Yale University School of Medicine *F SHM C435 *F 333 Cedar St. *F New Haven, CT 06510 *F 203-785-6078 # *U FBrf0155420 *a Selvaraj *b A. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 09:50:58 2003 *F To: anand@molbio.unizh.ch *F Subject: Helping FlyBase: ADRC-10018 *F Dear Anand, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Central role of 'metal transcription factor' (MTF-1) in metal homeostasis *F revealed by targeted mutagenesis in D.melanogaster. *F You mention genes that are new to FlyBase, MtnC and MtnD. Do you know which of *F the Genome Project CG annotations your genes correspond to? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If your genes do not correspond to CGs then *F perhaps you could tell me their genomic location, as this is valuable *F information for the genome annotation project. As it happens an MtnD has *F recently been introduced to our dataset, representing CG33192 \- though this *F may not have been public when you wrote your abstract. Is this the same gene *F as your MtnD? *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From anand@molbio.unizh.ch Mon Feb 10 16:51:26 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10018 *F Dear Rachel, *F Thanks for your note. *F MtnC has a CG number and it is CG5097 *F The accession number for MtnD is AF546903 in gene bank. *F I visited the CG number (FlyBase) you wrote me and it is the same MtnD and *F actually i wrote to flybase about this MtnD last year and it seems they *F have given it a CG number. But i haven`t got any updated information from *F them. Anyway, I think it is clear now. *F will be happy to answer any further clarification. *F regards *F anand *F anand selvaraj *F Ph.D student *F Institute of Molecular Biology *F University of Zurich *F Winterthurer strasse 190, 8057 Zurich *F Switzerland *F Ph: 0041-1-6353173 *F Fax: 0041-1-6356830 *F Res: Frohburg Strasse 200 *F 8057 Zurich *F Ph: 0041-1-3643878 *F Mobile: 0041-0765855910 # *U FBrf0155421 *a Landis *b G. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:14:15 2003 *F To: landis@usc.edu *F Subject: Helping FlyBase: ADRC-10171 *F Dear Gary, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A search for doxycycline-dependent mutations that increase Drosophila life *F span identifies the VhaSFD, Sugar baby, filamin, fwd and Cct1 genes. *F You mention a gene symbol that is new to FlyBase, Sugar-baby. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F You also mention Red-herring, in the intron of enc. The Release 3 *F genome annotation for enc shows no intronic gene, so we have missed *F this one. The gene models for Red-herring and enc need to be *F incorporated into the genome annotation. It would be great if you could *F submit the gene structure information to FlyBase, either by using the *F update form for the genome annotation for enc e.g. at *F http://www.fruitfly.org/cgi-bin/annot/fban?FBan0010847 or by submitting *F to me or flybase-help@morgan.harvard.edu the sequences for the genes. *F These will be fed into the pipeline for reannotation of enc and be *F taken into account when it is next updated. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From landis@usc.edu Mon Feb 10 23:28:08 2003 *F Subject: Re: Helping FlyBase: ADRC-10171 *F To: Rachel Drysdale (Genetics) *F Dear Rachel, *F We just published an article in Genome Biology Online 'A search for *F doxycycline-dependent mutations that increase Drosophila life span identifies *F the VhaSFD, Sugar baby, filamin, fwd and Cct1 genes', which explains all the *F things you want to know, with maps! *F Please see G.Landis, D. Bhole, and J.Tower in the recent edition of that *F journal. *F There is other info in there that you will want to know as well. *F Sincerely, *F Gary Landis. *F P.S.-Sugary Baby corresponds to CG7334 # *U FBrf0155422 *a O'Day *b M. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:17:50 2003 *F To: lissa95@imap2.asu.edu *F Subject: Helping FlyBase: ADRC-10248 *F Dear Melissa, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Identification and Characterization of persephone, a Gene that *F Codes for a Transcription Factor in Drosophila melanogaster. *F You mention the CG30011 gene, which you have named persephone. *F Unfortunately you will not be able to use this gene symbol as there *F already is a gene called psh:persephone (FBgn0030926). Sorry to be the *F bearer of bad tidings. Please could you choose a different symbol:name *F for CG30011 and let me know what it is so that I can enter it into the *F database as soon as possible. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From lissa95@imap2.asu.edu Mon Feb 10 21:53:01 2003 *F Subject: Re: Helping FlyBase: ADRC-10248 *F To: Rachel Drysdale *F Dear Rachel, *F Sorry, I did not know that persephone was already being used. I renamed the *F CG30011 gene. The new name is gem:gemini. *F Thank you for your patience, *F Melissa # *U FBrf0155423 *a Edwards *b K. *t 2003.2.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:03:42 2003 *F To: kaedwar@ilstu.edu *F Subject: Helping FlyBase: ADRC-10855 *F Dear Kevin, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Is extra eye a tyrosine phosphatase? *F You write about a gene, GEF, at 26CD. Is this Gef26 (FBgn0021873)? If *F not, do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kaedwar@ilstu.edu Mon Feb 10 14:44:30 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10855 *F I was referring generically to the proteins encoded by genes to avoid *F using their names. The genes referred to are Gef26 and Ddr, which *F encodes the Discoidin Domain Receptor-class RTK. *F I was avoiding the name 'Gef26' since Qi He's lab uses the synonym *F 'dizzy' for this gene. We used 'dizzy' on his poster last year, but I *F will keep using the more descriptive 'Gef26' until 'dizzy' gets into *F the literature. *F Kevin *F \-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= *F Dr. Kevin A. Edwards *F Asst. Prof. of Genetics *F Dept. of Biological Sciences *F Illinois State University *F Box 4120 *F Normal, IL 61790-4120 *F Office 309-438-7689 *F Fax 309-438-3722 *F Lab 309-438-5075 *F http://www.bio.ilstu.edu/Edwards *F kaedwar@ilstu.edu *F \-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= *F From rd120@gen.cam.ac.uk Tue Feb 11 15:08:10 2003 *F To: kaedwar@ilstu.edu *F Subject: Re: Helping FlyBase: ADRC-10855 *F Hi Kevin, *F Thanks for this \- all very interesting. Your mail raises several *F things all of which give me a chance to improve the FlyBase files. *F First of all dizzy/Gef26. dizzy corresponds to CG9491 and, you say, is *F the same gene as Gef26 (for which a correlation with a genome *F annotation had not been established). Great. I will merge the gene *F records for Gef26 and dizzy (both of which currently exist in *F FlyBase). Your mail below indicates that you favour dizzy as the gene *F symbol. I notice that Gef26 has precedence but as far as I can see you *F are the only author to have used Gef26 so it can become dizzy if that *F is OK with you. *F Secondly \- I note that you have sent us a personal communication before *F (FBrf0149572) concerning the annotation of CG9488/CG9490 and things *F between, which you would like to see named Ddr for 'Discoidin Domain *F Receptor-class RTK'. The curator who dealt with that personal *F communication suffered from a lack of nerve and did not merge the five *F annotations into one gene record because you had not named each one *F explicitly. Are they CG9488, CG13985, CG11573, CG13984 and CG9490? *F When you confirm this to be the case I will finally bring about the *F merge and rename the merged gene Ddr. *F Sorry to answer your helpful reply with more questions, but if I have *F this all correct we will be able to reduce seven FlyBase gene records *F to two, both of which are tied to the genome, and that would be very *F satisfying. *F Best regards, *F Rachel. *F From kaedwar@ilstu.edu Wed Feb 12 00:21:29 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10855 *F > *F > *F >First of all dizzy/Gef26. dizzy corresponds to CG9491 and, you say, is *F >the same gene as Gef26 (for which a correlation with a genome *F >annotation had not been established). Great. I will merge the gene *F >records for Gef26 and dizzy (both of which currently exist in *F >FlyBase). Your mail below indicates that you favour dizzy as the gene *F >symbol. I notice that Gef26 has precedence but as far as I can see you *F >are the only author to have used Gef26 so it can become dizzy if that *F >is OK with you. *F I would not go with 'dizzy' until it is published and the name is *F clearly linked with the phenotype. Let's stick with Gef26 for now *F since that is more descriptive for the broader signaling community. *F > *F >Secondly \- I note that you have sent us a personal communication before *F >(FBrf0149572) concerning the annotation of CG9488/CG9490 and things *F >between, which you would like to see named Ddr for 'Discoidin Domain *F >Receptor-class RTK'. The curator who dealt with that personal *F >communication suffered from a lack of nerve and did not merge the five *F >annotations into one gene record because you had not named each one *F >explicitly. *F Let's call it 'Discoidin domain receptor (Ddr)'. *F >Are they CG9488, CG13985, CG11573, CG13984 and CG9490? *F My predicted aa seq (not based on cDNA, there still is no EST *F available) includes sequences from CG9488, CG13985, CG11573, and *F CG9490. It does not include CG13984, which may be a spurious ORF. I *F did not look in detail at the genomic arrangement of the predicted *F genes on a genome browser, you are probably better equipped to do *F that. I can resend my predicted aa seq if necessary. # *U FBrf0155424 *a Grewal *b S. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:02:12 2003 *F To: ssgrewal@fhcrc.org *F Subject: Helping FlyBase: ADRC-10797 *F Dear Savraj, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Ribosomal RNA synthesis and the regulation of cell growth. *F You mention a gene symbol that is new to FlyBase, Tif-IA. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From ssgrewal@fhcrc.org Mon Feb 10 22:35:36 2003 *F Subject: Re: Helping FlyBase: ADRC-10797 *F To: Rachel Drysdale (Genetics) *F Hi Rachel *F The gene I am referring to is known as TIF-IA in mammals and rrn3 in *F yeast. I identified it as the drosophila homolog by sequence *F alignment. The CG number is CG3278 *F take care *F Savraj # *U FBrf0155425 *a Read *b R. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:09:20 2003 *F To: rdread@artsci.wustl.edu *F Subject: Helping FlyBase: ADRC-10992 *F Dear Renee, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Drosophila ortholog of C-terminal Src kinase regulates cell growth and *F proliferation through Stat92E. *F You mention a gene symbol that is new to FlyBase, Csk. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F Is it CG17309? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From rdread@artsci.wustl.edu Mon Feb 10 17:30:10 2003 *F To: Rachel Drysdale *F Subject: Re: Helping FlyBase: ADRC-10992 *F Dear Ms. Drysdale, *F The Drosophila ortholog of C-terminal Src kinase, or dCsk, is indeed *F CG17309. Let me know if you need any more information. *F Renee Read *F Department of Molecular Biology and Pharmacology *F Washington University School of Medicine *F 660 S. Euclid Avenue *F St. Louis, MO 63110 *F lab: (314)-362-7797 *F fax: (314)-362-7058 *F rdread@artsci.wustl.edu *F On Mon, 10 Feb 2003, Rachel Drysdale wrote: # *U FBrf0155426 *a Kim *b C. *t 2003.2.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:03:24 2003 *F To: changgk@hanwha.co.kr *F Subject: Helping FlyBase: ADRC-10841 *F Dear Changsoo, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Nanchung and Inactive are TRPV channel subunits required for hearing. *F You mention a gene symbol that is new to FlyBase, nanchung. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. Do you have an abbreviation that you *F will use for this gene? Now would be a good time to introduce it to *F FlyBase. I have just checked and, at time of writing, nch is available *F for you to use as the short symbol for nanchung \- let me know if you *F think this is suitable. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From changgk@hanwha.co.kr Tue Feb 11 00:13:29 2003 *F To: 'Rachel Drysdale (Genetics)' *F Subject: ReHelping FlyBase: ADRC-10841 *F nanchung corresponds *F to CG5842. RT-PCR cloned gene contains one additional exon than CG5842. *F I named it nanchung since it is deaf (Korean for deafness). *F I used nan for abbri. for nanchung in the paper submitted recently. *F Hope that this helps you. *F with best wishes, *F chang # *U FBrf0155427 *a Crews *b S. *t 2003.2.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:15:43 2003 *F To: ljiang@imap.unc.edu *F Subject: Helping FlyBase: ADRC-10233 *F Dear Lan, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Dysfusion control of tracheal fusion. *F You mention a gene symbol that is new to FlyBase, dys. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From steve_crews@unc.edu Tue Feb 11 17:56:26 2003 *F To: *F Subject: RE: Helping FlyBase: ADRC-10233 *F Hi Rachel, *F Dysfusion (Dys) is a bHLH-PAS protein that corresponds to CG32474. *F Initially, CG14552, CG14553, CG14554, and CG12561 comprised this gene, *F and it was later coalesced into CG32474. I had also contributed the *F name 'cranky', but since we have gone on to show that dysfusion plays a *F role in tracheal fusion, I renamed it. Since no publications or *F abstracts have used 'cranky', I felt it was preferable to rename the *F gene, while recognizing this is generally not good behavior. We have *F submitted a manuscript describing our initial molecular, cellular, and *F genetic work on dys. *F Cheers, *F Steve *F \------------------------------------------------ *F Stephen Crews *F Professor *F The University of North Carolina at Chapel Hill *F Department of Biochemistry/PMBB *F CB#3280 Fordham Hall *F Chapel Hill, NC 27599-3280 *F (919) 962-4380 (Tel) *F (919) 962-8472 (Fax) *F steve_crews@unc.edu *F http://www.unc.edu/~crews # *U FBrf0155428 *a Wei *b H.C. *t 2003.2.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:12:37 2003 *F To: hweica@yahoo.ca *F Subject: Helping FlyBase: ADRC-10115 *F Dear Ho-Chun, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of the phosphoinositide phosphatase Dsac1 in Drosophila *F melanogaster. *F You mention a gene symbol that is new to FlyBase, Sac1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From hweica@yahoo.ca Wed Feb 12 16:34:05 2003 *F Subject: Re: Helping FlyBase: ADRC-10115 *F To: Rachel Drysdale *F HI Rachel *F Sorry for the delayed reply. The corresponding CG for *F Sac1 is CG9128. I looked up the Flybase but there was *F no name for CG9128. I carefully analyzed this gene *F sequence and found it is a homolog of yeast Sac1p and *F thus I named it as Dsac1. Please let me know if there *F is any problem with it. Thank you. *F Yours truly *F HoChun # *U FBrf0155429 *a Tanaka-Matakatsu *b M. *t 2003.2.11 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:08:57 2003 *F To: miho@mail.nih.gov *F Subject: Helping FlyBase: ADRC-10986 *F Dear Miho, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Shattered encodes Anaphase Promoting Complex-1 (APC-1) and regulates G1 arrest *F in developing eye. *F You indicate that you know which of the Genome Project CG annotations *F shtd corresponds to. If you could let me know then I will merge the gene *F records for the CG and shtd. All the CGs have corresponding gene records *F in FlyBase already and we don't like to keep duplicate records for what *F is actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From miho@mail.nih.gov Wed Feb 12 01:12:12 2003 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10986 *F Dear Dr. Drysdale, *F I appreciate your effort to updating Fly base. *F We are working for APC-1, it is CG9198. However, I am pretty surprised. I *F checked AE003499 genomic sequence a week ago, I found two alternative *F transcripts reported to CG9198. They are CG9198-RA(log form) and *F CG9198-RB(short form). We have APC-1 mutant, it is rescued by entire CG9198-RA *F ( long *F form), and shtd1 and shtd3 mutants have each mutation on N-terminal that is *F covered only CG9198-RA. Unfortunately, when I made CG9198 transgenic fly, *F it was 2 years ago, I did not have information about CG9198-RB. I did not make *F transgenic fly for short form CG9198-RB neither performed phenotype *F rescue. *F Thank you *F Miho Tanaka-Matakatsu # *U FBrf0155430 *a Bhattacharya *b A. *t 2003.2.12 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:24:17 2003 *F To: bhatta@waksman.rutgers.edu *F Subject: Helping FlyBase: ADRC-10441 *F Dear Ananya, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A phosphatase inhibitor-3 functions in the NF-kB/Rel pathway in embryonic *F polarity and immunity. *F You mention a gene symbol that is new to FlyBase, R9-28. We already *F have an allele in FlyBase with as similar name \- l(1)19Ec8 \- is this *F the same allele as yours? *F Also, do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From bhatta@waksman.rutgers.edu Wed Feb 12 21:47:59 2003 *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: RE: Helping FlyBase: ADRC-10441 *F Dear Rachel, *F By R-9-28 we mean that gene mutant of which was referred to as l(1)19Ec8. *F We are currently working on the publication where we have have changed the *F name R-9-28. The CG number for this is CG11233. *F Hope this clears the confusion. *F Ananya # *U FBrf0155431 *a Sharma *b Y. *t 2003.2.10 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:15:23 2003 *F To: yashoda-sharma@uiowa.edu *F Subject: Helping FlyBase: ADRC-10223 *F Dear Yashoda, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Does dynein heavy chain 36D perform intraflagellar transport in Drosophila? *F I'm wondering whether you have, by now, shown whether or not btv *F corresponds to Dhc36C. If they do both so I will merge the gene *F records for btv and Dhc36C in FlyBase. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From ysharma@blue.weeg.uiowa.edu Tue Feb 11 00:33:02 2003 *F To: Rachel Drysdale (Genetics) *F Subject: RE: Helping FlyBase: ADRC-10223 *F Hi, *F The gene Dhc36C has absolutely nothing to do with the gene on talking *F about in my poster, 36D. They are two different genes, mine is CG15148 *F in Fly Base. And our results indicate that CG15148 is not btv. Does this *F help? *F Yashoda # *U FBrf0155432 *a Isaac *b E. *t 2003.2.13 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:00:58 2003 *F To: r.e.isaac@leeds.ac.uk *F Subject: Helping FlyBase: ADRC-10775 *F Dear Richard, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Neprilysin (NEP) family of zinc metallopeptidases and peptide signaling in *F Drosophila melanogaster . *F You mention a gene symbol that is new to FlyBase, Nep4. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From r.e.isaac@leeds.ac.uk Thu Feb 13 09:14:57 2003 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10775 *F NEP4 is CG4058. *F \-- *F Professor R. Elwyn Isaac *F Faculty of Biological Sciences *F School of Biology *F Miall Building *F University of Leeds *F Leeds LS2 9JT *F U.K. *F Office Telephone: \+44-(0)113 343 2903 *F Lab Telephone: \+44-(0)113 343 2904 *F FAX: \+44-(0)113 343 2835 *F Email: r.e.isaac@leeds.ac.uk # *U FBrf0155433 *a Welte *b M. *t 2003.2.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:06:27 2003 *F To: welte@brandeis.edu *F Subject: Helping FlyBase: ADRC-10059 *F Dear Michael, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A determinant for directionality of organelle transport in Drosophila embryos. *F It is clear from your abstract that you now know which genome *F annotation corresponds to halo. Great. If you could let me know which *F it is I'll merge the gene records for the annotation and halo. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From welte@brandeis.edu Mon Feb 17 06:16:43 2003 *F Subject: Re: Helping FlyBase: ADRC-10059 *F To: Rachel Drysdale (Genetics) *F Hi Rachel, *F Sorry for not responding right away, but I am swamped with preparing *F for the meeting. The Halo data aren't yet published, but as I *F understand giving Flybase this info shouldn't affect a future *F publication. Is that correct? *F If so, here is what we found: Halo corresponds to CG7428. *F Best, *F Michael *F Michael Welte, Ph.D. *F Assistant Professor *F Department of Biology MS029 *F Rosenstiel Center, Room 332 *F Brandeis University *F 415 South Street *F Waltham, MA 02454 *F Tel. (781) 736-2445 *F Lab (781) 736-2391 *F Fax. (781) 736-4793 *F e-mail welte@brandeis.edu *F http://www.bio.brandeis.edu/weltelab # *U FBrf0155434 *a Yanicostas *b C. *c N. *d Raich *t 2003.2.3 *T personal communication to FlyBase *u phtf expression in Drosophila testis [figure: Northern blot]. *F Archived. # *U FBrf0155435 *a He *b Y. *t 2003.2.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:05:02 2003 *F To: hy826@hotmail.com *F Subject: Helping FlyBase: ADRC-10927 *F Dear Ying, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F In vivo functional analysis of trc gene. *F You mention a gene symbol that is new to FlyBase, Mob1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From hy826@hotmail.com Mon Feb 17 14:54:02 2003 *F Subject: Re: Helping FlyBase: ADRC-10927 *F CG11711, which is homolog of s.cerevesiae Mob1. *F Ying *F Ying He, Ph.D Candidate *F Paul Adler Lab *F Gilmer Hall 245 *F Dept. of Biology *F University of Virginia *F Charlottesville, VA 22903 *F USA *F Tel : 1-434-982-5476 # *U FBrf0155436 *a Flores *b C. *t 2003.2.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:02:39 2003 *F To: wrengels@facstaff.wisc.edu *F Subject: Helping FlyBase: ADRC-10807 *F Dear Bill, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Double strand break repair: four pathways, many genes. *F You mention two genes that at face value are new to FlyBase, mus81 and *F ligase4. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From ccflores@facstaff.wisc.edu Mon Feb 17 16:33:21 2003 *F Subject: Re: Fwd: Helping FlyBase: ADRC-10807 *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F mus81 is CG3026 (EG:BACR7A4.16 ) *F and *F ligase4 is CG12176 *F Thanks very much for all your hard work! *F \-- *F Carlos Flores *F University of Wisconsin *F Lab of Genetics *F 445 Henry Mall *F Madison, WI 53706 *F (608) 262-5108 # *U FBrf0155437 *a Pusateri *b L. *t 2003.2.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:05:39 2003 *F To: pusateri@saturn.med.nyu.edu *F Subject: Helping FlyBase: ADRC-10932 *F Dear Leslie, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Maternal-Effect Screens Identify Mutants That Reduce Germ Cell Number. *F You mention genes that are new to FlyBase, shakelton, out-of-sync, *F bebra and poirot. Do you know which of the Genome Project CG *F annotations your genes correspond to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F Also, if you have short symbols for any of these genes, now would be *F a good time to tell me what they are and I can enter them correctly in *F the database from the outset. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From pusatl01@med.nyu.edu Mon Feb 17 16:37:27 2003 *F Subject: Re: Helping FlyBase: ADRC-10932 *F To: Rachel Drysdale *F Dear Dr. Drysdale, *F We are currently in the process of mapping the genes I mentioned: shakelton *F (shkl), out of sync (out), and bebra (beb). Therefore I do not yet know the CG *F annotations for these genes. poirot (prt) was previously published in *F Development, July 2002 as 'poirot, a new regulatory gene of Drosophila oskar *F acts at the level of the short Oskar protein isoform' by *F Sinka R, Jankovics F, Somogyi K, Szlanka T, Lukacsovich T, Erdelyi M. *F Thank you, *F Leslie Pusateri *F From rd120@gen.cam.ac.uk Tue Feb 18 10:36:02 2003 *F To: rd120@gen.cam.ac.uk, pusatl01@med.nyu.edu *F Subject: Re: Helping FlyBase: ADRC-10932 *F Dear Leslie, *F Thanks very much for getting back to me. Unfortunately out is already *F in use for outsiders (FBgn0063400), but shkl and beb are both fine. *F oos is available for out of sync, would you like to use that? We do now *F have poirot (valid symbol:name pcs:parcas) in our files though didn't *F when we first looked at the abstracts (our literature curation a little *F behind due to our exertions regarding genome reannotation). *F All the best, I'll go with 'oos' for 'out of sync' unless I hear from *F you otherwise. *F Rachel. *F From pusatl01@med.nyu.edu Tue Feb 18 19:09:02 2003 *F Subject: Re: Helping FlyBase: ADRC-10932 *F To: Rachel Drysdale *F Dear Dr. Drysdale, *F I did not know that out was already in use for outsiders, but oos is out of *F step/liprin-alpha, so I cannot use that. I looked up 'oosy' in Flybase and *F there are no matches, so let's just go with that for now. *F Thanks, *F Leslie *F From rd120@gen.cam.ac.uk Wed Feb 19 21:15:28 2003 *F Subject: Re: Helping FlyBase: ADRC-10932 *F To: pusatl01@med.nyu.edu *F Hi Leslie, *F as it happens oos is only in use as a synonym (Liprin-alpha being the *F valid symbol for FBgn0046704). *F That seems good enough reason to go with oosy for 'out of sync', to *F avoid any confusion, so I will, but I did just want to let you know *F that Liprin-alpha is the valid symbol for the 'other' oos. *F All the best , *F Rachel. # *U FBrf0155438 *a Daas *b S. *t 2003.2.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:01:50 2003 *F To: sajith.dass@uni-koeln.de *F Subject: Helping FlyBase: ADRC-10793 *F Dear Sajith, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The role of Cornichon (Cni) in subcellular transport of Gurken (Grk). *F You mention a gene symbol that is new to FlyBase, cnir. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Sajith.Dass@uni-koeln.de Mon Feb 17 18:02:31 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10793 *F Dear Rachel, *F Cornichon related (cnir) named so because of its *F sequence similarity with Cornichon (cni). It is CG17262. For the *F functional relatedness though i can only say it with absolute *F confidence once i have the loss of function allele (which i am trying *F to get). Also, if the loss of function has any interesting phenotype *F i would like to rename it with a more 'interesting name' (in the *F tradition of phenotype dependant nomenclature in fly research). *F Hope its not too late to answer your query, *F Thanks *F best regards *F Sajith *F 'The greatest danger for most of us is not that we aim too high and *F we miss it, but that it is too low and we reach it.' *F \-Michaelangelo *F Sajith Dass T , *F Prof. Siegfried Roth AG, *F Institut fur Entwicklungsbiologie, *F Universitat zu Koeln, *F Gyrhofstrasse. 17 (Lindenthal), *F D-50923 Koeln, *F Tel no: 0221-470-3167 (lab) *F 0021-470-3158 (fly-lab) *F 0179-1354030 (cell) *F Fax no: 0221-470-5164 (lab) # *U FBrf0155439 *a Nagengast *b A.A. *c A.S. *d Chaouki *t 2003.2.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 09:56:40 2003 *F To: sami@po.cwru.edu *F Subject: Helping FlyBase: ADRC-10577 *F Dear Ahmad, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F SIN and SPP: two proteins that link SXL to the general splicing machinery to *F block splicing of the Sxl male exon. *F You mention a gene symbol that is new to FlyBase, Spp. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From sami@po.cwru.edu Mon Feb 17 18:43:09 2003 *F Subject: Re: Helping FlyBase: ADRC-10577 *F To: Rachel Drysdale (Genetics) *F Hi Rachel--sorry for the delay; in fact, it is my colleague, Alexis *F Nagengast's, project which concerns SPP. The annotation corresponding to *F SPP (SNF's Protein Partner) is CG6525. Thanks *F A Sami Chaouki # *U FBrf0155440 *a Laurencon *b A. *t 2003.2.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:19:13 2003 *F To: Laurencon@maccgmc.univ-lyon1.fr *F Subject: Helping FlyBase: ADRC-10292 *F Dear Anne, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Drosophila Regulatory Factor X is necessary for ciliated sensory neuron *F differentiation. *F Your work studies Drosophila homologs of osm-6 , osm-5, and osm-1. We *F need to know which these genes are, so that we can add your reference *F to the correct genes in our files. Is the osm-6 gene our CG9595 *F (FBgn0031829)? Do you know which of the Genome Project CG annotations *F your other two genes corresponds to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. We *F can use the symbols osm-6 , osm-5, and osm-1 for the fly genes, as they *F have not (yet) been used for any other gene. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Laurencon@cgmc.univ-lyon1.fr Tue Feb 18 08:22:39 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10292 *F Hello Rachel, *F Sorry for the delay, here are the genes *F osm-6: CG9595 *F osm-5: CG12548, nompB *F osm-1: CG13809 *F Best regards, *F \-- *F Anne Laurencon *F Centre de Genetique Moleculaire et Cellulaire *F Universite Claude Bernard Lyon1/ UMR 5534 *F 16 rue Dubois *F 69622 VILLEURBANNE Cedex *F ph: (0)472-446-260 *F fax: (0)472-440-555 # *U FBrf0155441 *a Beller *b M. *t 2002.3.17 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:19:30 2003 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 10 Feb 2003 10:19:30 \+0000 *F To: mbeller@gwdg.de *F Subject: Helping FlyBase: ADRC-10298 *F Cc: rd120@gen.cam.ac.uk *F X-Sun-Charset: US-ASCII *F From: Rachel Drysdale (Genetics) *F Date: Mon, 10 Feb 2003 10:19:31 \+0000 *F Content-Length: 1095 *F Dear Mathias, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Fat storage is controlled by Perilipin-like lipid droplet protein LSD2 in *F Drosophila. *F You mention a gene symbol that is new to FlyBase, Lsd2. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From mbeller@gwdg.de Mon Feb 17 17:00:24 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10298 *F Dear Rachel, *F Excuse me very much for the late answer! The gene we call LSD2 corresponds *F to the annotated gene CG9057. *F With best wishes, *F Mathias Beller *F Mathias Beller *F Max-Planck-Institut für biophysikalische Chemie *F Abteilung Molekulare Entwicklungsbiologie *F Am Fassberg 11 *F 37077 Göttingen *F phone: 0551 / 2011657 *F mobile: 0179 / 5136251 # *U FBrf0155442 *a Sokol *b N. *t 2003.2.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:03:04 2003 *F To: nicholas.sokol@dartmouth.edu *F Subject: Helping FlyBase: ADRC-10817 *F Dear Nicholas, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Functional Analysis of microRNAs in Drosophila. *F You mention genes that are new to FlyBase, mir-34, mir-100 and mir-125. *F Do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. Given the nature of *F these genes, it is not unlikely that they won't have corresponding CG/CR *F genes, in which case perhaps you could send us sequence so that we *F could get them correctly annotated for the genome annotation project. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From Nicholas.S.Sokol@Dartmouth.EDU Tue Feb 18 02:52:48 2003 *F Subject: Re: Helping FlyBase: ADRC-10817 *F To: rd120@gen.cam.ac.uk *F Dear Rachel, *F Here is some hopefully useful information about the genes listed above: *F mir-34 *F sequence: GGCAGTGTGGTTAGCTGGTTG *F predicted hairpin precursor: *F TTGGCTATGCGCTTTGGCAGTGTGGTTAGCTGGTTGTGTAGCCAATTATTGCCGTTGACAATTCACAGCCACTATCTTC *F ACTGCCGCCGCGACAAGCTAA *F location:AE003685 *F mir-34 was identified based on it sequence similarity to C. elegans mir-34, *F reported in Lau, Lim, Weinstein, and Bartel, 2001, SCIENCE, 294:858-862, and *F its expression in flies has been confirmed by Northern blot. *F mir-100 *F sequence: AACCCGTAAATCCGAACTTGTG *F predicted hairpin precursor: *F CCATTAACAGAAACCCGTAAATCCGAACTTGTGCTGTTTTATATCTGTTACAAGACCGGCATTATGGGAGTCTGTCAAT *F location: AE003659 *F mir-100 was identified based on it sequence similarity to human mir-100, *F reported in Mourelatos et al., 2002, GENES AND DEVELOPMENT, 16:720-728, and *F its expression in flies has been confirmed by Northern blot. *F mir-125 *F sequence: TCCCTGAGACCCTAACTTGTGA *F predicted hairpin precursor: *F GTTTGTATGGCTGATTCCCTGAGACCCTAACTTGTGACTTTTAATACCAGTTTCACAAGTTTTGATCTCCGGTATTGGA *F location: AE003659 *F mir-125 was identified based on it sequence similarity to mouse mir-125, *F reported in Lagos-Quintana, Rauhut, Yalcin, Meyer, Lendeckel and Tuschl, 2002, *F CURRENT BIOLOGY, 12: 735-739, and its expression in flies has been confirmed *F by Northern blot. A Northern blot of mir-125 expression in flies has also *F been published in the Current Biology paper mentioned above. *F Incidentally, mir-100 and mir-125 flank the fly let-7 sequence: mir-100 is *F about 500 bp upstream of let-7 and mir-125 is about 300 bp downstream of *F let-7. All 3 have similar expression patterns raising the possibility that *F they are contranscribed from the same promoter. *F Hope this helps, *F Nick *F _____________________ *F Nicholas Sokol, Ph.D. *F Dartmouth Medical School *F Department of Genetics *F Vail 608 *F Hanover, NH 03755 *F Phone: 603/650-1940 *F Fax: 603/650-1188 # *U FBrf0155443 *a Hiller *b M. *t 2003.2.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 09:57:22 2003 *F To: chenxin@cmgm.stanford.edu *F Subject: Helping FlyBase: ADRC-10613 *F Dear Xin, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Tissue specific transcription machinery regulates gene expression program for *F spermatid differentiation. *F You mention a gene symbol that is new to FlyBase, rye. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From mhiller@goucher.edu Tue Feb 18 15:50:31 2003 *F Subject: RE: Helping FlyBase: ADRC-10613 *F To: 'Rachel Drysdale (Genetics)' *F Hello, *F Sorry I've been slow to reply. The CG number for ryan express (rye) is *F CG15632. *F Mark *F Mark Hiller *F Department of Biological Sciences *F Goucher College *F Baltimore, MD 21204-2794 *F Phone: (410)-337-6306 *F E-mail: mhiller@goucher.edu # *U FBrf0155444 *a Johansen *b K. *t 2003.2.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:16:02 2003 *F To: uttama@iastate.edu *F Subject: Helping FlyBase: ADRC-10235 *F Dear Uttama, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Mitotic requirement for a functional complex of spindle matrix proteins in *F Drosophila . *F You mention a gene symbol that is new to FlyBase, Megator. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Also, if you have settled on a short symbol for your gene now would be *F a good time to let me know what it is, as then I can enter it in the *F database from the outset. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kristen@iastate.edu Tue Feb 18 16:28:59 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10235 *F Dear Rachel, *F My graduate student forwarded your e-mail below regarding Megator. *F Megator is the name we have settled on for the Bx34 antigen (which *F until now did not have a name, and is currently listed in FlyBase *F under the antibody that was used to identify it.) There is no CG *F number assigned to it that I could tell. The best short symbol that *F was still available appears to be mtor. Please let me know if there *F is any problem with that abbreviation that I didn't notice. And, of *F course, let me know if you have any other questions! *F Best regards, *F Kristen Johansen *F Kristen M. Johansen, Ph.D. *F Professor of Zoology and Genetics *F 3154 Molecular Biology Building *F Iowa State University *F Ames, Iowa 50011 *F phone:515-294-7959 *F fax: 515-294-4858 *F e-mail: kristen@iastate.edu *F From kristen@iastate.edu Tue Feb 18 17:39:54 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10235 *F >Hi Kristen, *F > *F >Thank you for getting back to me on this one. I've looked at the Bx34 *F >record and notice two things. Firstly \- it is associated with a CG *F >annotation \- CG8274. Does this make sense to you? Secondly, I see *F >that it has been discussed under the name of Tpr. Since Bx34 is really *F >an antibody designation we should have renamed the gene Tpr, but due to *F >some oversight or other we did not. I will now, and that means that Megator *F >will become a synonym of Tpr, as Tpr has precedence. *F > *F >Best regards, and thanks again for getting back to me, *F > *F >Rachel. *F > *F Hi Rachel- *F The reason why we did not name it Tpr is because there is little *F doubt that the Bx34 antigen is not the functional Tpr homolog. The *F overall domain organization of the two proteins is very similar but *F the sequence identity is quite low. Tpr appears to be restricted to *F NPC and nuclear rim, whereas the Bx34 antigen is also very abundant *F intranuclearly. (There has been a very vigorous debate about Tpr in *F the nucleus, and it appears that the major labs involved finally all *F agree that Tpr is not found intranuclearly.) So that is why Michael *F Paddy originally resisted naming the protein Tpr. He had mentioned *F to me that FlyBase had contacted him because the gene needed a name, *F but he wasn't ready to name it at that time because he was unsure of *F its true identity or function. We have been collaborating on this *F project when we found Megator interacted with Skeletor .... and so we *F have named it to reflect its functional association with the spindle *F matrix. This appears to be very different than what Tpr's function *F is in mammalian nuclei. (Tpr does not go to the spindle.) So *F although it is true that the Bx34 antigen has sometimes erroneously *F been called 'Drosophila Tpr', it is probably very misleading to name *F the gene Tpr because it does not appear to be the same in either *F sequence or function, just in overall domain organization. *F However, of course FlyBase should do what they think is best, but *F this was the rationale for Michael and I giving it another name. And *F I suspect if it is 'officially' named Tpr the confusion will continue. *F Let me know what you ultimately decide to do, and of course I'll be *F happy to answer any other questions or make any clarifications that *F would help you in deciding how to handle the naming. *F Thanks in advance! *F Best regards, *F Kristen *F Kristen M. Johansen, Ph.D. *F Professor of Zoology and Genetics *F 3154 Molecular Biology Building *F Iowa State University *F Ames, Iowa 50011 *F phone:515-294-7959 *F fax: 515-294-4858 *F e-mail: kristen@iastate.edu # *U FBrf0155445 *a Johansen *b K. *t 2003.2.18 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:16:21 2003 *F To: yunji@iastate.edu *F Subject: Helping FlyBase: ADRC-10240 *F Dear Yun, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F D-hillarin, a novel LIM-domain containing protein in the fly CNS. *F You mention a gene symbol that is new to FlyBase, Hillarin. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F Also, if you have settled on a short symbol for your gene now would be *F a good time to let me know what it is, as then I can enter it in the *F database from the outset. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From kristen@iastate.edu Tue Feb 18 16:37:11 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Helping FlyBase: ADRC-10240 *F Dear Rachel, *F Yun Ji forwarded the e-mail below to me. D-Hillarin is the *F Drosophila homolog to the leech Hillarin gene (accession \# AAK49949). *F It is currently identified in FlyBase under the following CG numbers: *F CG30147 *F CG10439 *F CG13435 *F We haven't chosen a short abbreviation yet but would propose either *F D-hil (to emphasize it's a homolog of hillarin) or just plain hil. *F Both of those options appear to be available, so we're willing to go *F with whatever is preferred by FlyBase. (Note that hillarin is *F localized to axon hillocks in leech, hence the name, but we don't yet *F know whether that will be true for the drosophila homolog.) *F Let me know if you have any other questions! *F Thanks in advance, *F Kristen Johansen *F Kristen M. Johansen, Ph.D. *F Professor of Zoology and Genetics *F 3154 Molecular Biology Building *F Iowa State University *F Ames, Iowa 50011 *F phone:515-294-7959 *F fax: 515-294-4858 *F e-mail: kristen@iastate.edu # *U FBrf0155446 *a Gatti *b M. *t 2003.2.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:09:45 2003 *F To: maurizio.gatti@uniroma1.it *F Subject: Helping FlyBase: ADRC-10996 *F Dear Maurizio, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F fascetto encodes a Drosophila homologue of PRC1 required for cytokinesis. *F You mention a gene symbol that is new to FlyBase, feo. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From maurizio.gatti@uniroma1.it Wed Feb 19 11:24:12 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10996 *F Hi Rachel, *F fascetto corresponds to CG11207. *F Regards *F Maurizio *F Maurizio Gatti, *F Dipartimento di Genetica e Biologia Molecolare *F Università di Roma 'La Sapienza' *F P. A. Moro, 5 00185 Roma, Italy *F Tel: 39-06-49912842; Fax: 39-06-4456866 # *U FBrf0155447 *a Sasamura *b T. *t 2003.2.24 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:18:51 2003 *F Envelope-to: rd120@gen.cam.ac.uk *F To: sasamura@rs.noda.tus.ac.jp *F Subject: Helping FlyBase: ADRC-10273 *F Dear Takeshi, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Drosophila maternal neurogenic gene, neurotic is essential for Notch *F signaling pathway. *F You mention a gene symbol that is new to FlyBase, ntc:neurotic. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From sasamura@rs.noda.sut.ac.jp Mon Feb 24 06:37:44 2003 *F To: rd120@gen.cam.ac.uk *F Subject: about neurotic *F Dear Rachel: *F I am sorry that my reply is too late. *F The CG annotation number of our mutant, neurotic is CG12366. *F This is found to be the same gene as OFUT1, which is reported by Okajima and *F Irvine. *F Sincerely, *F Takeshi Sasamura, PhD *F Matsuno Lab # *U FBrf0155448 *a Levis *b R. *t 2003.1.30 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Thu Jan 30 16:35:08 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: l(2)k08901 \- two insertions? *F The FlyBase record for P{lacW}l(2)k08901k08901 (synonym l(2)k08901) *F treats it as having a single P element insertion with a flanking *F sequence accession \# AQ034114. This accession number is for the 5' *F flanking sequence recovered by BDGP. BDGP also recovered a 3' *F flanking sequence, GenBank accession \# AQ025874, which is not given *F in the FlyBase record for l(2)k08901. The two flanks map 30 kb *F apart, suggesting either two insertions 30 kb apart or an insertion *F associated with a 30 kb deletion. The 3' flank to an intron of *F CG4798. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ *F From rd120@gen.cam.ac.uk Mon Feb 17 15:12:38 2003 *F To: flybase-help@morgan.harvard.edu, levis@ciwemb.edu *F Subject: Re: l(2)k08901 \- two insertions? *F Dear Bob, *F Gillian and I have looked into this now. Sorry to take so long. *F The gene record for l(2)k08901 does in fact have both accessions for *F k08901, AQ025874 and AQ034114. *F BLAST of AQ025874 maps to the large intron of CG4798. *F BLAST of AQ034114 maps to about 30kb distal, just 5' to BEST:GM02553. *F According to gbrowse the large intron of CG4798 includes the insertions k08901 *F (AQ025874) and k01209 (AQ025700). *F gbrowse shows no l(2)k08901 label for AQ034114 5' to BEST:GM02553. *F AQ025700 maps to the large intron of CG4798 *F So here is what I will do. I will reinstate l(2)k01209 as a gene and merge *F the record with that for CG4798. *F I will disentangle l(2)k08901 from l(2)k01209, and invent an *F aberration 'Df(2R)k08901'. One of the aberration breakpoints will be *F 'l(2)k01209k08901', which will have both a link to FBti0006619, and *F to the new aberration. *F Thanks for bringing this to our attention, *F All the best *F Rachel. # *U FBrf0155461 *a King *b O.D. *c J.V. *d White *e R.E. *f Foulger *g F.P. *h Roth *t 2002.9.23 *T personal communication to FlyBase *u *F frederick_roth@hms.harvard.edu *F oliver_king@hms.harvard.edu *F ref26@gen.cam.ac.uk *F Frederick Roth's group modeled relationships among GO terms to predict *F gene function based on patterns of annotations. Using existing *F annotations in FlyBase (from a snapshot taken in January 2002), they *F predicted GO terms that should be assigned to 50 fly genes. The *F predicted annotations were manually assessed and the predicted terms *F that were scored 'known to be true' and were more specific than the *F existing GO terms were included in FlyBase gene records. *F \---------------------------------------------------------------------- *F KEY: *F NAME: Gene name *F FBID: FBgn identifier for gene *F GOID: Identifier of predicted GO term *F TERM: Name of predicted GO term *F SCORE: Rating of 1 to 3 for if the GO term is likely *F 1 = Known to be true. *F 2 = Known not to be true. *F 3 = Neither of the above. *F NAME CG1268 FBID FBgn0035521 GOID GO:0000220 TERM C-hydrogen-transporting *F ATPase V0 domain SCORE 1 *F NAME CG1268 FBID FBgn0035521 GOID GO:0000221 TERM C-hydrogen-transporting *F ATPase V1 domain SCORE 2 *F NAME CG6905 FBID FBgn0035136 GOID GO:0003734 TERM F-small nuclear *F ribonucleoprotein SCORE 3 *F NAME CG4980 FBID FBgn0039558 GOID GO:0003734 TERM F-small nuclear *F ribonucleoprotein SCORE 3 *F NAME CG12775 FBID FBgn0032987 GOID GO:0003735 TERM F-structural protein of *F ribosome SCORE 1 *F NAME CG12740 FBID FBgn0035422 GOID GO:0003735 TERM F-structural protein of *F ribosome SCORE 1 *F NAME Hsf FBID FBgn0001222 GOID GO:0003773 TERM F-heat shock protein SCORE 2 *F NAME Ubi-p63E FBID FBgn0003943 GOID GO:0003773 TERM F-heat shock protein SCORE 3 *F NAME Thor FBID FBgn0022073 GOID GO:0003797 TERM F-antibacterial peptide SCORE 3 *F NAME REG FBID FBgn0029133 GOID GO:0004299 TERM F-proteasome endopeptidase *F SCORE 3 *F NAME TyrR FBID FBgn0004514 GOID GO:0004984 TERM F-olfactory receptor SCORE 3 *F NAME lush FBID FBgn0020277 GOID GO:0004984 TERM F-olfactory receptor SCORE 3 *F NAME CG4536 FBID FBgn0029904 GOID GO:0004984 TERM F-olfactory receptor SCORE 3 *F NAME Tl FBID FBgn0003717 GOID GO:0005044 TERM F-scavenger receptor SCORE 3 *F NAME bnk FBID FBgn0004389 GOID GO:0005200 TERM F-structural protein of *F cytoskeleton SCORE 1 *F NAME Cortactin FBID FBgn0025865 GOID GO:0005200 TERM F-structural protein of *F cytoskeleton SCORE 1 *F NAME CG4415 FBID FBgn0031296 GOID GO:0005563 TERM F-transfer RNA SCORE 2 *F NAME CG6905 FBID FBgn0035136 GOID GO:0005570 TERM F-small nuclear RNA SCORE 2 *F NAME CG4980 FBID FBgn0039558 GOID GO:0005570 TERM F-small nuclear RNA SCORE 2 *F NAME Tbh FBID FBgn0010329 GOID GO:0005792 TERM C-microsome SCORE 3 *F NAME CG5235 FBID FBgn0036565 GOID GO:0005792 TERM C-microsome SCORE 3 *F NAME CG7495 FBID FBgn0037154 GOID GO:0005792 TERM C-microsome SCORE 3 *F NAME Cyp12a2 FBID FBgn0040476 GOID GO:0005792 TERM C-microsome SCORE 3 *F NAME Cyp450_U5csm FBID FBgn0044501 GOID GO:0005792 TERM C-microsome SCORE 3 *F NAME Pros28.2 FBID FBgn0011827 GOID GO:0005839 TERM C-20S core proteasome *F SCORE 1 *F NAME CG1909 FBID FBgn0039911 GOID GO:0005892 TERM C-nicotinic *F acetylcholine-gated receptor-channel SCORE 1 *F NAME CG7972 FBID FBgn0035843 GOID GO:0006371 TERM P-mRNA splicing SCORE 1 *F NAME CG5931 FBID FBgn0036548 GOID GO:0006371 TERM P-mRNA splicing SCORE 1 *F NAME CG14060 FBID FBgn0036683 GOID GO:0006371 TERM P-mRNA splicing SCORE 1 *F NAME CG4980 FBID FBgn0039558 GOID GO:0006371 TERM P-mRNA splicing SCORE 1 *F NAME l(2)01424 FBID FBgn0010488 GOID GO:0006413 TERM P-protein synthesis *F initiation SCORE 1 *F NAME AGO1 FBID FBgn0026611 GOID GO:0006413 TERM P-protein synthesis initiation *F SCORE 1 *F NAME CG12413 FBID FBgn0039588 GOID GO:0006413 TERM P-protein synthesis *F initiation SCORE 1 *F NAME Ulp1 FBID FBgn0027603 GOID GO:0006514 TERM P-deubiquitylation SCORE 1 *F NAME trp&ggr; FBID FBgn0032593 GOID GO:0006816 TERM P-calcium ion transport *F SCORE 1 *F NAME kl-2 FBID FBgn0001313 GOID GO:0007018 TERM P-microtubule-based movement *F SCORE 1 *F NAME shi FBID FBgn0003392 GOID GO:0007018 TERM P-microtubule-based movement *F SCORE 3 *F NAME Cdic FBID FBgn0013761 GOID GO:0007018 TERM P-microtubule-based movement *F SCORE 1 *F NAME unc-104 FBID FBgn0034155 GOID GO:0007018 TERM P-microtubule-based *F movement SCORE 3 *F NAME CG1193 FBID FBgn0037375 GOID GO:0007018 TERM P-microtubule-based movement *F SCORE 3 *F NAME CG10845 FBID FBgn0039246 GOID GO:0007018 TERM P-microtubule-based *F movement SCORE 3 *F NAME CG6905 FBID FBgn0035136 GOID GO:0008248 TERM F-pre-mRNA splicing factor *F SCORE 1 *F NAME Voila FBID FBgn0024917 GOID GO:0008527 TERM F-taste receptor SCORE 3 *F NAME PK19 FBID FBgn0044025 GOID GO:0008527 TERM F-taste receptor SCORE 3 *F NAME PK11 FBID FBgn0044026 GOID GO:0008527 TERM F-taste receptor SCORE 3 *F NAME syd FBID FBgn0024187 GOID GO:0008567 TERM F-dynein ATPase SCORE 3 *F NAME Dhc1 FBID FBgn0025441 GOID GO:0008567 TERM F-dynein ATPase SCORE 1 *F NAME Unc-76 FBID FBgn0040395 GOID GO:0008567 TERM F-dynein ATPase SCORE 3 *F NAME cnn FBID FBgn0013765 GOID GO:0008570 TERM F-myosin ATPase SCORE 1 *F NAME bip2 FBID FBgn0026262 GOID GO:0016251 TERM F-general RNA polymerase II *F transcription factor SCORE 1 # *U FBrf0155462 *a Smith *b D. *t 2003.2.19 *T personal communication to FlyBase *u *F Date: Tue, 18 Feb 2003 17:41:45 \-0500 *F From: dean smith *F Subject: Lush *F To: flybase-help@morgan.harvard.edu *F MIME-version: 1.0 *F Content-transfer-encoding: 7BIT *F User-Agent: Microsoft-Entourage/9.0.2509 *F Lush is still listed as a G-coupled receptor-it is a soluble *F odorant-binding protein. *F Dean Smith # *U FBrf0157089 *a Levis *b R.W. *t 2002.11.20 *T personal communication to FlyBase *u *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Wed, 20 Nov 2002 16:10:26 -0500 *F To: crosby@morgan.harvard.edu *F From: Robert Levis *F Subject: Construction of pP{EPgy2} *F X-Spam-Status: No, hits=1.7 required=3.0 *F tests=SIGNATURE_LONG_SPARSE,SMTPD_IN_RCVD,SPAM_PHRASE_00_01 version=2.43 *F *F Lynn - Here is a draft of a personal communication describing the *F construction of pP{EPgy2}. Let me know if anything needs *F clarification. ...Bob *F *F P{EPgy2} is similar to P{EPg}. P{EPg} was first described in a *F publication by Mata et al. (2002) Cell 101: 511-522 (FBrf0128569) and *F the FlyBase ID for P{EPg} is FBtp0012862. The major differences are *F that P{EPgy2} contains an intronless yellow gene module and lacks the *F plasmid rescue module of P{EPg}. I constructed the plasmid pP{EPgy2} *F from two plasmid precursors, p1462 and yellow-BSX. *F *F 1) p1462 was obtained from Pernille Rorth (EMBL, Heidelberg). This *F was an intermediate used in the construction of pP{EPg}. She *F described p1462 to me as being the same as pP{EPg} except for the *F absence of the plasmid rescue module. *F *F 2) The yellow-BSX plasmid was obtained from Tim Parnell in the *F laboratory of Pamela Geyer (University of Iowa). It was described as *F having the SalI fragment of yellow-intronless inserted into the SalI *F site of a modified pBluescript vector, pBS-X. This vector has the *F KpnI site of the polylinker converted into an XbaI site. This yellow *F SalI fragment is the same as segment designated by FlyBase as *F y+mDint25.2(S,S) (FlyBase ID FBms0003824). *F *F I digested yellow-BSX DNA with a combination of NotI and PspOMI. *F PspOMI cleaves the same recognition sequence as ApaI (GGGCCC), but *F generates the same 5' overhang as NotI (GGCC). NotI cuts yellow-BSX *F at a single site in the polylinker sequences closest 3' end of the *F yellow gene and PspOMI cuts yellow-BSX at a single site in the *F polylinker sequences closest to the 5' end of the yellow gene. I *F gel-purified the 5.8 kb fragment containing the yellow gene. *F *F I ligated the NotI - PspOMI fragment of yellow-BSX with DNA from *F p1462 that had been cut by NotI. p1462 has a unique NotI site *F located between mini-white and the GAGA/GAL4-UAS modules. The yellow *F fragment can insert in either of two orientations into the NotI site *F of p1462. I recovered transformants in both orientations and named *F them pP{EPgy1} and pP{EPgy2}. *F -- *F ------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F ------------------------------------ *F *F Mime-Version: 1.0 *F X-Sender: levis@pop.ciwemb.edu *F Date: Wed, 20 Nov 2002 17:22:36 -0500 *F To: Madeline Crosby *F From: Robert Levis *F Subject: Sequences P{EPgy2} and p1462 *F X-Spam-Status: No, hits=-0.3 required=3.0 *F tests=BALANCE_FOR_LONG_20K,IN_REP_TO,REFERENCES, *F SIGNATURE_LONG_SPARSE,SMTPD_IN_RCVD,SPAM_PHRASE_00_01, SUPERLONG_LINE *F version=2.43 *F X-Spam-Level: *F *F Lynn, *F *F I'm attaching files of p1462 (including the plasmid vector) and EPgy2 *F (P-element only). Both were saved as text-only Word files in FASTA *F format. *F *F ...Bob *F ------------- End Forwarded Message ------------- *F *F *F --Array_of_Hedgehogs_970_000 *F Content-Type: TEXT/plain; name="p1462.txt"; charset=us-ascii *F Content-Description: p1462.txt *F Content-MD5: rAG/qKcYXsvPGKwUKbEKXw== *F *F >p1462 from 5'P, including plasmid vector *F catgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtg *F cacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtg *F gaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgc *F cgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaat *F ttgtgatacccactttaatgattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggagg *F cgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctata *F ccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgac *F tgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagta *F cgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaatt *F aaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccattttt *F ttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactc *F cgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaa *F atatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacga *F aatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtc *F gaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtg *F gtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgtttt *F atgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatcc *F aaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattgg *F cacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaa *F aaaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccag *F atatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcg *F gagaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaa *F tttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagag *F gtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaat *F ggtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatc *F cgaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggc *F ccgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggcaattcg *F gcaatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactg *F gcagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattgaaatgcaaatg *F tattctaaacatgacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaa *F gatggctccgttgatattcatcacacccacttgcgtgagttgttggcccaaaaagatgaggccaatcaag *F atggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcgcgagttaaatgaaatttatt *F tatcttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaatt *F ctatctaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccac *F tcaccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgcca *F caggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggc *F ttctccagatttttggtggccaacaactgctccatatcccgggctactttgctaatggcaaaattgtcgc *F atatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaa *F gtccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgttatgtaatac *F tagatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaag *F aaagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgat *F gaatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggt *F aaaggagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagagcctcggaggcg *F aatgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgct *F gacatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatg *F tcgtggcatccgcaccatggcttggaaaatcaggtgttccctggccgttagggagccgataaagaggtca *F tcctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgca *F tcccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttcc *F ggcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaatt *F ggtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgag *F caaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctg *F ccgccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttc *F tcggctagttggccgctccctgaaccggagtcctccggcggactgggtggccggagcgtgccgtagtttt *F tggcctgcccgaagccctggttaatgcagctctgcgaagccgctccgctgtcaccctgcaatgatagggg *F atctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaaagtaccccgaagtatcctacg *F aagtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattt *F tttgtaaaaaagaatatagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatctcaaaaatt *F gtcttatatagcgagaacagctaccagaataatctgtttcgtgtcactatttgtttgtgcaattgcggtt *F tgggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatc *F aagtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctg *F cctccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttac *F ccacccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttgg *F cacagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaaatgc *F aactgaaggcggacattgacgctacgtaacgctacaaacggtggcgaaagagatagcggacgcagcggcg *F aaagagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattt *F tgagtgagaggtaatcgaaagaacctggtacatcaaatacccttggatcgaagtaaatttaaaactgatc *F agataagttcaatgatatccagtgcagtaaaaaaaaaaaatgttttttttatctactttccgcaaaaatg *F ggttttattaacttacatacatactagaattcggtacccgcccggggatcagatccgcggccgctctagc *F cccccctcgaatgttctctctcttctcttctctctctctttctcgaatgttctctctcttctcttctctc *F tctctttctcgaggtcatcaagcttaggcctccaaggcggagtactgtcctccgggctggcggagtactg *F tcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgt *F cctccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtc *F ctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgtcc *F tccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcct *F ccggcaagggtcgagtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgt *F tgagaggaaaggttgtgtgcggacgaatttttttttgaaaaccggtgatagagcctgaaccagaaaagat *F aaaagaaggctataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgta *F aacaataacgtgactgtgcgttaggtcctgttcattggtaccctcgaggtcgacctgcagccaagcttat *F gagttaattcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactca *F atacgacactcagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttg *F ccgacgggaccaccttatgttatttcatcatggtctggccattctcatcgtgagcttccgggtgctcgca *F tatctggctctaagacttcgggcccgacgcaaggagtagccgacatatatccgaaataactgcttgtttt *F ttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgtaattatattt *F gtgccaataaaaacaagatatgacctatagaatacaagtatttccccttcgaacatccccacaagtagac *F tttggatttgtcttctaaccaaaagacttacacacctgcataccttacatcaaaaactcgtttatcgcta *F cataaaacaccgggatatattttttatatacatacttttcaaatcgcgcgccctcttcataattcacctc *F caccacaccacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcaccttttg *F ttcgacgaccttggagcgactgtcgttagttccgcgcgattcggtgcggtatttcacaccgcatatggtg *F cactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgac *F gcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgca *F tgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctattttt *F ataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcgga *F acccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaa *F tgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttt *F tgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcag *F ttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccg *F aagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgc *F cgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcaca *F gaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataaca *F ctgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatggg *F ggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgac *F accacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagctt *F cccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttcc *F ggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactg *F gggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaac *F gaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactc *F atatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgat *F aatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatca *F aaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctacc *F agcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactgacttcagcagagcg *F cagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgc *F ctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgg *F gttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacag *F cccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgc *F ttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga *F gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcga *F tttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccttcttcttgaactcgggctcggtgc *F cagtatacctcaaatggttgtcgtacctctcatggttccgttacgccaacaagggtctgctgattaacca *F atgggcggacgtgaagccgggcgaaattagctgcacatcgtcgaacaccacgtgccccagttcgggcaag *F gtcatcctggagacgcttaacttctccgccgccgatctgccgctggactacgtgggtctggcc *F *F --Array_of_Hedgehogs_970_000 *F Content-Type: TEXT/plain; name="EPgy2.txt"; charset=us-ascii *F Content-Description: EPgy2.txt *F Content-MD5: pOPzyyw68QmrH/f3FW658A== *F *F >EPgy2 P element, from 5'P end *F catgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtg *F cacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtg *F gaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgc *F cgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaat *F ttgtgatacccactttaatgattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggagg *F cgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctata *F ccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgac *F tgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagta *F cgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaatt *F aaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccattttt *F ttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactc *F cgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaa *F atatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacga *F aatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtc *F gaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtg *F gtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgtttt *F atgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatcc *F aaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattgg *F cacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaa *F aaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccaga *F tatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcgg *F agaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaat *F ttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagagg *F tacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaatg *F gtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatcc *F gaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggcc *F cgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggcaattcgg *F caatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactgg *F cagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattgaaatgcaaatgt *F attctaaacatgacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaag *F atggctccgttgatattcatcacacccacttgcgtgagttgttggcccaaaaagatgaggccaatcaaga *F tggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcgcgagttaaatgaaatttattt *F atcttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaattc *F tatctaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccact *F caccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgccac *F aggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggct *F tctccagatttttggtggccaacaactgctccatatcccgggctactttgctaatggcaaaattgtcgca *F tatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaag *F tccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgttatgtaatact *F agatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaaga *F aagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgatg *F aatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggta *F aaggagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagagcctcggaggcga *F atgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgctg *F acatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatgt *F cgtggcatccgcaccatggcttggaaaatcaggtgttccctggccgttagggagccgataaagaggtcat *F cctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgcat *F cccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttccg *F gcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaattg *F gtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgagc *F aaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctgcc *F gccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttctc *F ggctagttggccgctccctgaaccggagtcctccggcggactgggtggccggagcgtgccgtagtttttg *F gcctgcccgaagccctggttaatgcagctctgcgaagccgctccgctgtcaccctgcaatgataggggat *F ctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaaagtaccccgaagtatcctacgaa *F gtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattttt *F tgtaaaaaagaatatagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatctcaaaaattgt *F cttatatagcgagaacagctaccagaataatctgtttcgtgtcactatttgtttgtgcaattgcggtttg *F ggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatcaa *F gtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctgcc *F tccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttaccc *F acccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttggca *F cagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaaatgcaa *F ctgaaggcggacattgacgctacgtaacgctacaaacggtggcgaaagagatagcggacgcagcggcgaa *F agagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattttg *F agtgagaggtaatcgaaagaacctggtacatcaaatacccttggatcgaagtaaatttaaaactgatcag *F ataagttcaatgatatccagtgcagtaaaaaaaaaaaatgttttttttatctactttccgcaaaaatggg *F ttttattaacttacatacatactagaattcggtacccgcccggggatcagatccgcggccgctctagaac *F tagtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtcgacctgcaggtcaacgga *F tcctaccctaccaccaacaaagtctttttgtttgccccgtctgaagtctggctccgatcaaaagcaaaaa *F tttcaattacagtcgaatctcaagcgaccaggcgatctcaaattgagcgattaagttgaactttccctgc *F acccaaacaagatcttgtgcatagctttatcaacctactttcttgcctggtagtagtactccagaaaatg *F tacagttgttgatatatttagaatttatgcatacttacattttttccgctttttccgctcaagaaaattg *F cgtaaactcttaaccttcaaaaaaaagttgatttattgttattttttgcttaacataactagataaagta *F ttgatttgccacttgctcatacgtcatgtggtttttttaaccgctgttgccctatgttgttgtctttaaa *F tattctttacatcaatcgagtgtgtgagaatatacccaagtaccgtgtgtaggattatgttaaccttgat *F gctgatgatgccaccacccagattggcgggcattcacataagtttttaacccaccatcctggccacccgg *F ttgggcgggttgatgggtgggaaatagatggggaccacttgtctcgtaatttatgccgttgtgctggttg *F aaaatataggcctttggcacctccactcctgcaggacccacagaatttgtagagacactaatactggaga *F ctacattgcctgaattggcgggcaaataagtgcgacttggaggaggcggcgaggaagcccagctggtctg *F aggtttctgtggcaagacaggacgatattgtttcgtatataacggtggacccattggcaaaacggcttgt *F tttggtattgaaacggtatttggcggcccataggcgttattcctcaaatcacacacagtattctcaatta *F aagtggccaagggagccgtgtaaattcggaaattagtatctgaataatccaagtcagacagcaagaaaac *F gggcatcctatcggatagaacccaaacgtttttgttctcatcaattttcacatcggccggaaaaactaag *F ccaacgtcatcgcgatccacaatgccatgaaattgcggtgagtacggcattgatgagtgccagcaaccca *F ctgcattttgatctattaaattgaacagctcaattccatcatcgctcatcacacgtgaagtggtatggga *F gtttggaccccgttcatctaaggcaacaaagtcatgatagctatcttccgtcctggtttcatccctcaaa *F atcctcgtggatacggcaaattgtcgatgacttgctaacggactaaagtacagggtacgataaccatccg *F atcgaatgggcgaaagggacataccaaatataccctcctcgccccattggaagttaataccagcgacatt *F gaaatcgcccctcaatggatcggggaaaaaatacgaatgtgccgagaatctccaggacttgttcagttcc *F caggagtaagcaatcaagccgtatcccaattcatcggcaaaataggcatatgcatcatcgcaatttttgc *F ctatatccacggcaatgttagctatgaaagtatttggatttgtgtccacgccaggtagctcgtatctccg *F aattcgcgtatccgtggtcaagtcaaagacatttaccgcataggggcacggattagtggtggtattgccg *F atgcccacggttccagtgtccaaaacccacagccgaccacactcatccactttaatgcggtaggcagtgg *F taatactgttggcgcaatctccagctgtatttgagcgccaatctggatacggaattagctccggtgaacc *F cgtcaaactgcggtccatgtttatataggtcagagtggccggaatcccatcacgccagcggggaacagtg *F acgaataaccgattgccaaagtgttcgactccaacaggtagagcattttgcggaatataatctccactag *F ccagagcttggtcctttagtcgggtattcgggaaagcaaagtccagctggctccaactatatcgctcctg *F aagtttgtaagcagcccaagacggcgtcaccaaggtgatcagggtcacaaggatccaccctttgtcctgg *F aacattgcacttagctctaagctgacaatcactcttaacacttaagccaaagtgtgttggcaataaaaaa *F aaatatatttgtttatatatgttcagctatagggggttcttctctagccttcggctgtgtgatatttttt *F taatcctcttctgtggaccgtggcgcggtaacgactggtggccataatatgtcggccgcgttttatatga *F aggtttttttctccgaagacgaagacaggccaatgaaaatgaaaacgaaggcgcgcgccaacttcggtgg *F ttttagtgattcgggtggttcagtgttcgggtaatcaggtggcttatgctgttcccatagatcggccaac *F tttgcgttttgtcttccatgattgattttcacgcatgtttaataaatacactcgaatcccccaatttaat *F gagtcagacatcacctttcgctgggtttggtatgatttttggccttcatcgacatttaaaactgcgctga *F tttgtgccgaaatacctttcggtaccctaaaagattaagacctattttaaagttgtgactactatttatt *F taattttagggactttatttgacgatctaatttcaaacaagtaaattgtaaggccaggaggctcgtgcat *F agaatgcattgctctccaaatttctgaaaatctgaaagcttaatacatgacagttgtgttctgagaactg *F tgttcatctttatcggcgactgcaaaaaatgttctaatatgattttttagttcaattcattaaaaaaatc *F aaatcaatattatatgaaatacatgcagacaaaaatccagaaatatcaaataaatcgttattataacgtg *F tactatgtagagtatttaaagcaggtgtttccttgtatactattaaattggaactcgtgctcgtttcaat *F tgcttaggattggatttcgattgggcgtcaccgatgattcacaattcactgagggtcaaatatttggttt *F ccgctagttattggcaggtgattttgagcatacatatacataaatataattgagtgttacataaaatttc *F aaaaaggaacgaaagcgcttttaggtaattccactagctgttatttaaaatattgtggtccctgaaaatc *F aactggatgttgggggtgccctaagtgggtaatgctctaatgaccatcgccgcaagtaaatgttaaaaat *F tcgaaccgaaagcgatcgcatatatctcttggcccaaatgcacctgcttgctaaagaaaaaattgcaaaa *F cttaagtaaagtaaacaaccagcgacacgcataataaacatgcaaatgggtaaaaatacgttttacgacc *F tccgcaggtaaagtggtattgcattaatcctcaataatctacactttaaataaggaaaacctctcaaaat *F attaaaatacaaattaaagaatgccgtttaaatttaatcttatttcttcggatgtctaaaaacaatttca *F aagctaatgtaaccattactgctattgaattggtgtagtttttggactctatatcatttatgcaatgtct *F attttttcttagaagtggttcaacttgatcccttgcccgcattctaaatcattgcacccacagaaaaaat *F aaacaaacacggtgtggcaatcgaaaaatatgttgcacaaaattaccggcaatgctgctcgaatttattg *F cgatcggcaggcttattaaaagacacacttgaaataaccagtacagttttattaattaattgtgtgtgtt *F gtgtaatgaaatggtgtatttactaaaaacgcaactaatgatgcgatcgctgcagccgttgaacctataa *F tttttttcgaattgacttgtttcaagtttttgcttaattgtgtgggggaatgctgcgtttgtctgtttgg *F ttactgtttttctttttcgcttttcagagcttgcggcaatgatttatacgcacttttcccaataattgaa *F acaagttttctgccccagcacaaaacaaaacaacatcagcggagcgcgctaagtggtcaaaaaaagcttc *F cacaaatcagatctctttggccataattgaccaacgcttatgcaaccgaccggcaacattcgacattatt *F tacacacaggatcacttcaacttgggcggaaagcaaagccgaataccgcatgacattgcccgacccgtgt *F ttcatgttttattttccttattccccacttatggcagcctggacattatctccctccctcccctcccccc *F tcctacactaatttggaaatccatcgtgactgcaaaacgaaaaacccgttgctcaacttctctaacataa *F gcggtacacagcattttccgttttgtgttatatatttttgtgttttttttttttcaagttcaatgtccat *F ggccctgaccgccgggtcccctcctttcgagctgggattatttggcatggccaaattcggaaggccgcga *F tcacttcccgcttacacacaattgcacatgtccccatgacatgcactgcgctcaaactattagtacccct *F tagtaacttaagccaaattaaaaataagttcattaatataaataaaatagtcaatttccactgtcccgat *F tgaatagagaaactttatttgtaaattttaaatgtcgccaaggaactcgctagatatactaaaccaaaac *F cgattgtagctcccgaaatcgcaaccataagtggaataattagatgccaacaaaggctttgtttgcttac *F tgaataaaggttgtcgtaatttgatttctgtgtaatcttgcacgaaacggaatcattacctcagacataa *F tttgcctgcaattgcggtggtgcacttgtctcgtgcaagcgaaaaaatcagtgctcgaaaaagcttagtg *F gatatactcgtacatatactatgaatcgtttagaggggtaggtaaatcagcgggctgcgttcgaaattta *F tgagtgactctgcgacgtatttatcttaagccatcgtcagttgcatattttatggctattttaaaccact *F caatgtggtaatcgggcgataatcatttaatagtcgacctcgagggggggccgctctagccccccctcga *F atgttctctctcttctcttctctctctctttctcgaatgttctctctcttctcttctctctctctttctc *F gaggtcatcaagcttaggcctccaaggcggagtactgtcctccgggctggcggagtactgtcctccggca *F aggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggct *F ggcggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaag *F gcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggctgg *F cggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggg *F tcgagtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgttgagaggaaa *F ggttgtgtgcggacgaatttttttttgaaaaccggtgatagagcctgaaccagaaaagataaaagaaggc *F tataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacg *F tgactgtgcgttaggtcctgttcattggtaccctcgaggtcgacctgcagccaagcttatgagttaattc *F aaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactcaatacgacact *F cagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttgccgacgggac *F caccttatgttatttcatcatg *F --Array_of_Hedgehogs_970_000 *F Content-Type: TEXT/plain; name=NoName; charset=us-ascii *F Content-Description: NoName *F Content-MD5: HCi0qs2rF+7Y4svz+WbtjA== *F *F -- *F ------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F ------------------------------------ *F # *U FBrf0157149 *a Khush *b R. *t 2003.2.25 *T personal communication to FlyBase *u *F Date: Mon, 24 Feb 2003 14:46:31 \-0800 *F To: Chihiro Yamada *F From: Ranjiv Khush *F Subject: Re: FlyBase Query (cy1790) *F Hi Chihiro, *F You've picked out some mistakes in the paper that I've corrected *F below. If you can note these corrections as a personal communication *F to flybase, I would appreciate it. *F Thanks, *F Ranjiv *F >Dear Dr Khush, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Khush et al., 2002, Curr. Biol. 12(20): 1728--1737 *F > *F >I have a few questions I was hoping you could answer for me. *F > *F >dCullin1 *F >======== *F >In your materials and methods you describe an allele, *F >dCullin1l(2)02074. This confuses me for a number of reasons. *F > *F >The only gene we have in our records that has been called Cullin1 or *F >dCul1 is a gene which is called lin19 in FlyBase, aka CG1877 or *F >l(2)k01207. This has been placed at 43F. This gene was called dCul1 *F >in Bocca et al., 2001, Biochem. biophys. Res. Commun. 286(2): 357--364 *F > *F >We currently have a gene called l(2)02074, which has an allele *F >l(2)0207402074, caused by an insertion, P{PZ}l(2)0207402074. This *F >has been placed at 39EF by insitu. I BLASTed the flanking sequence for *F >this insertion, and again it is placed at 39-40. *F > *F >Which annotation/gene do you mean when you use the symbol dCullin1 in *F >your Curr. Biol. paper? *F > *F >Do you mean the gene at 39EF which is affected by the insertion *F >P{PZ}l(2)0207402074, or do you mean CG1877 at 43F? *F > *F >Do you have data on the P{PZ}l(2)0207402074 insertion that FlyBase *F >does not know about? Any more information you have about this would be *F >greatly appreciated. *F I made a mistake: l(2)02074 is an insertion in cul-2 or CG1512. The *F mutation that I described in the paper is l(2)k01207, the insertion *F in dCullin1. *F .. *F Ranjiv Khush *F Department of Microbiology and Immunology *F Stanford University School of Medicine *F Stanford, CA 94305-5124 USA *F phone: (650) 724-8064 *F FAX: (650) 725-6757 *F email: khush@stanford.edu # *U FBrf0157150 *a Alexandre *b C. *t 2003.2.28 *T personal communication to FlyBase *u *F Date: Tue, 11 Mar 2003 20:58:22 \+0000 *F To: Chihiro Yamada *F From: Cyrille *F Subject: Re: FlyBase Query (cy1797) *F ... *F > GFP-Arm *F > ======= *F > You describe GFP-Arm construct, which you got from C. Alexandre. Do *F > you have any more molecular details on this construct? Is it a 'true' *F > mini-gene, i.e. constructed from genomic DNA. Is (as the name suggests) *F > the GFP inserted at the N terminus? What marker does the transposon *F > carry? *F just to recap: *F The GFP cDNA has been fused to the N terminus of Arm cDNA. The *F mini-gene arm pro-GFPARM has been created by cloning the fusion *F downstream of 2 kb arm promoter. The mini-gene has been inserted in *F pCasper4 so it's with white as a marker. *F \-- *F Cyrille ALEXANDRE *F Division of Mammalian Development, Room 108 *F National Institute for Medical Research (N.I.M.R) *F The Ridgeway, Mill Hill *F LONDON NW7 1AA *F UK *F Tel: \+44(0)208 816 2017(lab) *F Fax: \+44(0)208 816 2109 *F e-mail: calexan@nimr.mrc.ac.uk # *U FBrf0157151 *a Badenhorst *b P. *t 2003.3.11 *T personal communication to FlyBase *u *F Date: Wed, 12 Feb 2003 12:06:55 \-0500 *F Subject: Fwd: FlyBase Query (cy1763) (fwd) *F Cc: Carl Wu *F To: cy200@gen.cam.ac.uk *F From: 'Paul Badenhorst' *F Hi Chihiro, *F I answer to your questions... *F Df(3L)XZB970 was reported in Rebay et al, Genetics 154(2), 695-712, *F 2000. It complements trachealess, and fails to complement nurf301 and *F CG7036. *F Df(3L)rH321 is originally from the Berkeley P-element insertion *F collection, known as l(3)rH321. Matt Voas inverse PCRed off the 5' and *F 3' ends of this insertion and realized that they lie about 50kb apart. *F The proximal end is to the right of the nurf301 coding region and the *F distal end is to the right of the CG7036 coding region. He confirmed *F by PCR that this region is deleted rather than having two insertions *F that would confound the inverse PCR results. Genetically, Df(3L)rH321 *F fails to complement nurf301 but complements CG7036. *F Df(3l)3643 is an excision line derived from EP(3)3643. It removes *F nurf301 entirely. I mapped the proximal to within 2 kb of the nurf301 *F ATG. It leaves CG7020 intact. Distally, the deficiency removes at *F least to CG17142, however this breakpoint has not been precisely *F mapped. *F I hope this helps. *F Sincerely, *F Paul. *F > \---------- Forwarded message \---------- *F > Date: Tue, 11 Feb 2003 18:21:49 \+0000 *F > From: Chihiro Yamada *F > To: carlwu@helix.nih.gov *F > Cc: cy200@gen.cam.ac.uk *F > Subject: FlyBase Query (cy1763) *F > *F > Dear Dr Wu, *F > *F > I am currently curating your paper for FlyBase: *F > *F > Badenhorst et al., Genes Dev. 2002 16(24), 3186--3198 *F > *F > I have a few question I was hoping you could answer for me. *F > *F > Df(3L)XZB970 *F > ============ *F > We don't have a Deficiency of this name in our records. Has it been *F > published before? If so under what name? *F > *F > *F > Df(3L)rH321 *F > =========== *F > We don't have a Deficiency of this name in our records. Has it been *F > published before? If so under what name? What relationship does it *F > have to the insertion P{PZ}l(3)06240rH321, Was this insertion used to *F > make this line by imprecise excision? *F > *F > Df(3L)3643 *F > ========== *F > You make this Deficiency from P{EP}EP3643. Do you any information *F > about the breakpoints in this Df? Are either of them within the *F > nurf301 gene? *F > *F > Any new information you give us will be curated as a personal *F > communication form you to FlyBase, if thats fine with you. *F > *F > Best wishes, *F > *F > Chihiro *F > *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F > Department of Genetics, *F > University of Cambridge, email: c.yamada@gen.cam.ac.uk *F > Downing Street, Tel : 01223-333963 *F > Cambridge, CB2 3EH, FAX : 01223-333992 *F > United Kingdom. Memes don't exist. Spread the Word. *F > \---------------------------------------------------------------------- *F Paul Badenhorst *F NCI Laboratory of Molecular Cell Biology *F Bldg 37 Rm 6066 *F 37 Convent Drive *F Bethesda MD 20892-4255 *F USA # *U FBrf0157186 *a Brodsky *b M.H. *t 2003.5.16 *T personal communication to FlyBase *u *F Date: Fri, 16 May 2003 10:19:57 \-0400 *F Subject: Re: FlyBase Query (cy1883) *F From: Michael Brodsky *F To: Chihiro Yamada *F CC: *F Dear Chihiro, *F The rescue construct is a genomic fragment containing the 5' intergenic *F region and the transcribed region. Here is an excerpt from our paper *F currently under review. *F The genomic position of the amplified region of mnk is indicated in Figure *F 2. The oligonucleotides used for amplification were as follows: 523 bases 5¹ *F to the start of transcription, GGCCTCTAGAAACGACGCCGCAATTTAGGGC; 72 bases 3¹ *F to the end of transcription, GGCCGCGGCCGCTGAGCAATTTGCCCGCCTCCG. The *F underlined sequences correspond to XbaI and NotI sites that were used to *F clone the genomic fragment into the pCaSpeR-2 transformation vector. *F Regards, *F Mike Brodsky *F > Dear Drs Therkauf and Brodsky, *F > *F > I'm currently curating: *F > *F > Takada et al., 2003, Cell 113(1): 87--99 *F > *F > I have a quick question I was hoping you could answer for me. *F > *F > In this paper Takada et al. use a 'mnk-rescued' construct, which was *F > obtained from M.H. Brodsky. Can you give me some more molecular *F > details for this construct? Is it a genomic fragment, a mini-gene, or *F > a promoter driven mnk cDNA? *F > *F > I'm happy to wait for Brodsky's own paper for full details, however, I *F > need some details to minimally curate this allele, in order to ensure *F > that it is tracked between both papers. *F > *F > Any new information you give us will be curated as a personal *F > communication from you to FlyBase. *F > *F > Best wishes, *F > *F > Chihiro. *F > *F > \---------------------------------------------------------------------- *F > Chihiro Yamada. *F > *F > FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F > \---------------------------------------------------------------------- # *U FBrf0157193 *a Yang *b X. *t 2003.2.27 *T personal communication to FlyBase *u *F From mcbyangn@imcb.nus.edu.sg Thu Feb 27 02:40:21 2003 *F Envelope-to: djs93@gen.cam.ac.uk *F Subject: Re: Queries for FlyBase Curation. *F Dear David, *F Here are my answers in bold: *F >Firstly, could you check that the following are correct: *F > *F >sna/esg/wor triple mutants = sna1; esgG66 double homozygous embryos, *F >injected with wor RNAi *F san/esg/wor/=sna1, esgG66B double homozygous embryos injected with wor *F RNAi. *F >sna/esg/wor/pins embryos = sna1,esgG66 double homozygous, that are *F >also maternally and zygotically homozygous for rapsP62, injected with *F >wor RNAi. *F sna/esg/wor/pins embryos = Df(2L)TE35BC-3; pins62 *F >pins mutant embryos = Embryos both maternally and and zygotically *F >homozygous for rapsP62 or rapsP89. *F It should be rapsP89 in our paper. But both alleles are null and showed *F identical phenotype. *F >Secondly, on pg.55 you write: *F > *F >'In the majority of metaphase pins NBs ectopically expressing Galphai *F >(66%, n = 40), DaPKC remains localized as an apical crescent, although the *F >intensity is weak compared to wt (not shown). When zygotic Pins is present *F >under the same conditions (embryos derived from the same cross), the *F >frequency of symmetric NB divisions increases dramatically (82%, n=48).' *F > *F >Am I correct to infer that the pins mutant neuroblasts were both *F >maternally and zygotically homozygous mutant for Pins. The other progeny *F >you also clarify which pins (Raps) allele you used for this experiment? *F Yes. The allele used in this experiment is pins89. *F >Finally, it is not clear to me whether you used sca-Gal4 or neur-Gal4 in *F >the following cases: *F > *F >'When C-terminal Pins (C-Pins) containing the GoLoco motifs (Yu et al., *F >2002 ) are overexpressed in wt embryos...' Pg 55 *F For this experiment, C-Pins is under the control of a heatshock promoter. *F >'In the majority of metaphase pins NBs ectopically expressing Galphai'. Pg 55 *F For this experiment, Galphai ectopic expression was driven by the sca-gal4. *F >'To ascertain the role for Pins in the symmetric NB divisions caused by *F >Galphai overexpression, we overexpressed Galphai in pins mutant NBs' Pg 55 *F Driven by the sca-gal4. *F >'To explore this possibility, we expressed Pins-C-Pon in insc mutant *F >embryos' Pg 56 *F Driven by the sca-gal4. *F >'When we examined telophase pI ectopically expressing Insc after labeling *F >the centrosomes' Pg 57 *F Driven by the sca-gal4. *F >'When Insc is ectopically expressed in ventral epithelial cells of the *F >neuroectoderm, Pins/Galphai and Insc/Baz localize to the apical cortex of *F >the epithelial cell' *F >Pg 58 *F Driven by the sca-gal4. *F Please let me know if you other questions. *F Cheers, Xiaohang # *U FBrf0157203 *a Eissenberg *b J. *t 2003.4.16 *T personal communication to FlyBase *u *F In response to my query: *F 'From djs93 Wed Apr 16 15:51:34 2003 *F To: eissenjc@slu.edu *F Subject: Curation query *F I am currently curating your recent paper (Eissenberg et al., 2002, PNAS, USA *F 99(15): 9894--9899) for FlyBase. Would it be possible for you to help me with *F clarify the following issue: *F The numbering of the Su(Tpl) sequence in figure 2, doesn't appear to match *F that used in the text for decribing the the changes seen in particular alleles. *F e.g. *F Su(Tpl)17: nonsense mutation \- encodes a C terminally truncated protein of *F 789 aa. *F From the figure that substitution is Q798@. *F In Su(Tpl)S-192 missense mutations: R917G & D919N in the text, In fig are *F R926G & D928N *F Which numbers should I use in curating the leisions?' *F Joel Eissenberg replied: *F 'From eissenjc@SLU.EDU Wed Apr 16 19:53:01 2003 *F Envelope-to: djs93@gen.cam.ac.uk *F Delivery-date: Wed, 16 Apr 2003 19:53:02 \+0100 *F Date: Wed, 16 Apr 2003 13:51:27 \-0500 *F From: joel c eissenberg *F Subject: Re: Curation query *F To: David Sutherland *F ... the numbers running down the left-hand side of the sequence should be, *F from top *F to bottom: 1, 89, 177, 265, 353, 441, 529, 617, 705, 793, 881, and 969. In *F addition, the position of the tick in the Su(Tpl)17 mutation is shifted to *F the right by one amino acid. Now, this makes mutation in Su(Tpl)17 a *F Q789stop mutation, and the protein would be 788 a.a. in length. *F For Su(Tpl)S-192, this would make the mutations you asked about R916G and *F D918N. In addition, the third mutation in this allele is S660L, which is *F correctly stated in the text. The text *F doesn't state the position of the XS-706 substitution, but it is G501S. *F The annotation of the S1 and S2 alleles are unchanged.' # *U FBrf0157205 *a Dow *b J.A.T. *t 2003.2.21 *T personal communication to FlyBase *u *F Date: Fri, 21 Feb 2003 20:50:17 \+0000 *F To: Aubrey de Grey *F From: Julian Dow *F Subject: Re: FlyBase Help Mail *F .. *F Yes, These should be merged. CAP2b is a peptide (from Manduca sexta) which *F was found to be active in Drosophila, and thus implied the presence of an *F endogenous Drosophila hormone. capa encodes two such peptides, with *F biological activity inseparable from Manduca CAP2b (as described in Kean, *F 2001). There are no other similar potential peptides within the genome, so *F we believe that capa encodes the endogenous Drosophila peptide. *F regards, Julian Dow *F \------------------------------------------------------------------ *F Prof. Julian A.T. Dow IBLS Division of Molecular Genetics, *F University of Glasgow, Glasgow G11 6NU, UK *F Phone: \+44 141 330 4616 FAX: \+44 141 330 4878 *F email: j.a.t.dow@bio.gla.ac.uk WWW: fly.to/tubules # *U FBrf0157206 *a Gilliland *b W. *t 2002.10.16 *T personal communication to FlyBase *u FlyBase error report for CG4869 on Wed Oct 16 13:42:49 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 16 Oct 2002 13:42:49 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: wdgilliland@ucdavis.edu *F Subject: FlyBase error report for CG4869 on Wed Oct 16 13:42:49 2002 *F Error report from William Gilliland (wdgilliland@ucdavis.edu) *F Gene or accession: CG4869 *F Release: 3 *F Missed gene *F Comments: CG4869 appears to be betaTubulin97EF (FlyBase ID FBgn0003890). *F CG4869 is the only significant blast similarity to another Drosophila beta *F tubulin (betaTubulin56D) near 97EF, which was indicated as the site of a beta *F tubulin by situ hybridization. The predicted transcript (1879 nt) is also *F close to the reported transcript size (1.8 kb, Northern data). # *U FBrf0157207 *a Leopold *b P. *t 2003.3.18 *T personal communication to FlyBase *u *F To: leopold@unice.fr *F Subject: FlyBase query: abstract 1038C *F From: Gillian Millburn (Genetics) *F Date: Tue, 18 Mar 2003 13:10:35 \+0000 *F Dear Dr. Leopold, *F I am curating your abstract from the addendum of the 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 1038C: A nutrient sensor mechanism controls Drosophila growth. *F You mention a gene that is new to FlyBase, 'slimfast'. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F Also, do you have a short symbol that you use for the gene, so that I *F can enter it into the database ? There is already a gene with the *F symbol 'slim' in the database, so you would not be able to use that *F symbol. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F \-------------------------------------------------------------- *F From: 'Pierre Leopold' *F To: 'Gillian Millburn \(Genetics\)' *F Subject: Re: FlyBase query: abstract 1038C *F Date: Tue, 18 Mar 2003 15:21:55 \+0100 *F Dear Gillian, *F slimfast (short symbol : slif) corresponds to CG11128 *F Thank you for taking care of this matter, *F Best *F Pierre # *U FBrf0157210 *a Nose *b A. *t 2003.3.25 *T personal communication to FlyBase *u *F From: 'Nose Akinao' *F To: ''Gillian Millburn \(Genetics\)'' *F Subject: RE: FlyBase query: abstract 1041C *F Date: Tue, 25 Mar 2003 16:30:49 \+0900 *F Dear Mr. Gillburn, *F Thank you very much for your mail. The 'formin3' gene that we described *F in our poster at the fly meeting corresponds to CG14824, CG8633 and *F CG18138. These three tandem CG genes were mistakenly annotated as *F different genes. Based on the cDNA analysis, we found that these three *F CG genes actually encode a single gene which we named formin 3. .. *F Best regards, *F Akinao Nose *F > \-----Original Message----- *F > From: Gillian Millburn (Genetics) mailto:gm119@gen.cam.ac.uk *F > Sent: Tuesday, March 18, 2003 10:21 PM *F > To: nose@bio.phys.s.u-tokyo.ac.jp *F > Cc: gm119@gen.cam.ac.uk *F > Subject: FlyBase query: abstract 1041C *F > *F > *F > Dear Dr. Nose, *F > *F > I am curating your abstract from the addendum of the 44th *F > (Chicago) Annual Drosophila Research Conference, for FlyBase. *F > *F > I am writing in connection with your abstract: *F > *F > 1041C: A novel formin homology protein implicated in tracheal *F > formation in Drosophila. *F > *F > You mention a gene symbol that is new to FlyBase, 'formin3'. *F > Do you know which of the Genome Project CG annotations your *F > gene corresponds to? All the CGs have corresponding gene *F > records in FlyBase already and we don't like to make *F > duplicate records for what is actually the same gene unless *F > we can't avoid it. *F > *F > thanks, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > \-------------------------------------------------------------- *F > # *U FBrf0157211 *a Chien *b C.T. *t 2003.3.19 *T personal communication to FlyBase *u *F From ctchien@gate.sinica.edu.tw Wed Mar 19 04:20:58 2003 *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query: abstract 1049B *F Date: Wed, 19 Mar 2003 12:24:50 \+0800 *F Dear Dr. Millburn, *F In release 3 of genome annotation, multiple is CG31719 and yumo is CG6187. *F CG31719 is merged by CG13143 and CG7475 of the release 2. *F multiple and yumo may correspond to 'Scim16', Sensitized chromosome *F inheritance modifiers . *F Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places *F 'Scim16' near/in 'CG13143' or 'CG6187'. (Genetics, 2001,157:1623). *F However, the insertion site is not clear. *F By sequence analysis, Multiple and Yumo contain the pseudouridine synthase 2 *F domain which is shared by the RluA-family pseudouridine synthase of E. *F coli. *F Therefore, it may be more appropriate to rename multiple as RluA-1 and yumo *F as RluA-2. *F Thanks, *F CT *F Cheng-Ting Chien, Ph. D. *F Associate Research Fellow *F Institute of Molecular Biology *F Academia Sinica *F Taipei, Taiwan *F TEL:886-2-27899193 *F FAX:886-2-27826085 *F \----- Original Message \----- *F From: Gillian Millburn (Genetics) *F To: *F Cc: *F Sent: Tuesday, March 18, 2003 9:32 PM *F Subject: FlyBase query: abstract 1049B *F > Dear Dr. Chien, *F > *F > I am curating your abstract from the addendum of the 44th *F > (Chicago) Annual Drosophila Research Conference, for FlyBase. *F > *F > I am writing in connection with your abstract: *F > *F > 1049B: multiple encodes an RluA pseudouridine synthase-like protein in *F > multiple dendritic neurons in the Drosophila peripheral nervous *F > system. *F > *F > You mention 2 gene symbols that I think are new to FlyBase: 'yumo' and *F > 'multiple'. *F > *F > 1. yumo *F > *F > Do you know which of the Genome Project CG annotations yumo corresponds *F > to? All the CGs have corresponding gene records in FlyBase already and *F > we don't like to make duplicate records for what is actually the same *F > gene unless we can't avoid it. *F > *F > 2. multiple *F > *F > There is already a gene with the name 'multiple' (short symbol: mul) in *F > FlyBase, but this gene maps to chromosome 1, at 1B-1C. *F > *F > Your 'multiple' gene maps near to the E7-2-36 enhancer trap insertion *F > line, which according to Uemura et al., 1989, Cell 58: 349--360 maps to *F > chromosome 2. *F > *F > Please could you confirm that your 'multiple' gene is different from *F > the one in FlyBase. *F > *F > If it is different, could you tell me which CG it corresponds to. Also, *F > since the name 'multiple' is already taken by the gene on chromosome 1, *F > can you suggest a different name for your 'multiple' gene, *F > *F > thanks, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > *F > FlyBase: http://fly.ebi.ac.uk:7081/ *F > \-------------------------------------------------------------- # *U FBrf0157221 *a Acharya *b J. *t 2003.3.31 *T personal communication to FlyBase *u *F To: acharyaj@mail.ncifcrf.gov *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Mon, 31 Mar 2003 16:22:06 \+0100 *F Dear Dr. Acharya, *F I am curating your paper for FlyBase: *F Acharya et al., 2003, Science 299(5613): 1740--1743 *F and I have a question about the UAS-ceramidase construct you made. *F I would be grateful if you could tell me which of the CG gene *F predictions your 'ceramidase' corresponds to so that I make sure I put *F the construct under the correct gene in the database and can rename it *F to 'CDase'. *F In the 'Supporting Online Material' you state that it corresponds to *F 'EST SD007768', however, there are too many numbers in this EST number *F \- it should only have 5 numbers after the 'SD'. *F Did you mean 'EST SD07768' ? *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \-------------------------------------------------------------- *F Date: Mon, 31 Mar 2003 11:13:10 \-0500 *F To: Gillian Millburn (Genetics) *F From: Jairaj Acharya *F Subject: Re: FlyBase query *F Hello Dr.Millburn, *F The CDase we refer to is CG1471. The est we used is SD07768. *F Thank you, *F Jairaj Acharya *F >Dear Dr. Acharya, *F > *F >I am curating your paper for FlyBase: *F > *F >Acharya et al., 2003, Science 299(5613): 1740--1743 *F > *F >and I have a question about the UAS-ceramidase construct you made. *F > *F >I would be grateful if you could tell me which of the CG gene *F >predictions your 'ceramidase' corresponds to so that I make sure I put *F >the construct under the correct gene in the database and can rename it *F >to 'CDase'. *F > *F >In the 'Supporting Online Material' you state that it corresponds to *F >'EST SD007768', however, there are too many numbers in this EST number *F >- it should only have 5 numbers after the 'SD'. *F > *F >Did you mean 'EST SD07768' ? *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fly.ebi.ac.uk:7081/ *F >-------------------------------------------------------------- *F \-- *F Jairaj Acharya *F Principal Investigator *F National Cancer Institute, Frederick *F Room.22-6, Building 560 *F 1050 Boyle Street, Frederick, MD 21702 *F USA *F Ph:301-846-5299 *F Fax:301-846-1666 # *U FBrf0157229 *a Blanke *b S. *t 2003.4.2 *T personal communication to FlyBase *u *F Date: Wed, 2 Apr 2003 12:54:39 \+0200 *F Subject: CG for dRalBP *F Cc: hjaeckl@gwdg.de *F To: gm119@gen.cam.ac.uk *F From: Stephen Blanke *F Dear Gillian, *F the CG-number for 'dRalBP' is CG11622. dRalBP corresponds to RLIP *F mentioned unter FBgn0026056. *F Sincerely yours *F Stephen Blanke *F >> Dear Dr. Jackle, *F >> *F >> I am curating your paper for FlyBase: *F >> *F >> Beller et al., 2002, Mech. Dev. Gene Expression Patterns 2(3-4): *F >> 289--296 *F >> *F >> about the 'ima' gene and I have a question. *F >> *F >> In Figure 1A, you show the gene 'dRalBP'. Could you tell me which CG *F >> gene prediction this gene corresponds to so that I can rename it in *F >> the *F >> database, *F >> *F >> thanks, *F >> *F >> Gillian *F >> *F >> \-------------------------------------------------------------- *F >> Gillian Millburn. *F >> *F >> FlyBase (Cambridge), *F >> Department of Genetics, *F >> University of Cambridge, *F >> Downing Street, email: gm119@gen.cam.ac.uk *F >> Cambridge, CB2 3EH, Ph : 01223-333963 *F >> UK. FAX: 01223-333992 *F >> *F >> FlyBase: http://fly.ebi.ac.uk:7081/ *F >> \-------------------------------------------------------------- *F >> *F > # *U FBrf0157230 *a Stewart *b M. *t 2003.3.31 *T personal communication to FlyBase *u *F Date: Mon, 31 Mar 2003 20:03:44 \-0500 *F To: gm119@gen.cam.ac.uk *F From: Kevin Cook *F Subject: UAS-S6k insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mary Stewart, North Dakota State University (2/03). *F P{UAS-S6k.KQ}2, P{UAS-S6k.TE}2 and P{UAS-S6k.STDETE}2 are homozygous viable *F and fertile second chromosome insertions. *F P{UAS-S6k.STDE}3 is a homozygous viable and fertile third chromosome insertion. *F P{UAS-S6k.M}2 is a second chromosome insertion. *F The construction and transformation of these constructs was described in *F Stewart and Barcelo, genesis 34: 83-85, 2002. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157234 *a Fischer *b J.A. *t 2003.2.24 *T personal communication to FlyBase *u *F Date: Mon, 24 Feb 2003 22:36:05 \-0600 *F From: Janice Fischer *F To: flybase-help@morgan.harvard.edu *F Subject: stock problem *F BL stock number 11043 (l(2)k11511) is not an allele of lamin. It *F complements l(2)04643 and other lam alleles. It is actually inserted a *F few nt upstream of the tsn start of the adjacent gene, Hel25E. *F \-- *F Janice A. Fischer, PhD *F Associate Professor *F The University of Texas at Austin *F Section of Molecular Cell and Developmental Biology *F Institute for Cellular and Molecular Biology *F 1 University Station \- A4800 *F Austin, Texas 78712 # *U FBrf0157246 *a Grillo-Hill *b B. *t 2003.4.28 *T personal communication to FlyBase *u FlyBase error report for CG30474 on Mon Apr 28 13:57:25 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 28 Apr 2003 13:57:25 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: bree@genetics.wustl.edu *F Subject: FlyBase error report for CG30474 on Mon Apr 28 13:57:25 2003 *F Error report from Bree Grillo-Hill (bree@genetics.wustl.edu) *F Gene or accession: CG30474 *F Release: 3 *F Gene annotation error *F Gene CG30474 has incorrect exon/intron structure or translation start site. *F Comments: The annotation and protein prediction for CG30474 are based on the 5 *F prime read of 601bp of RH14220. There is additional 3 prime sequence available *F for this EST (and perhaps more sequence between), which likely affects the *F annotation. Thank you. # *U FBrf0157247 *a Bryce *b S. *t 2003.3.17 *T personal communication to FlyBase *u FlyBase error report for CG4049 on Mon Mar 17 06:52:58 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 17 Mar 2003 06:52:58 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: sbryce@hgmp.mrc.ac.uk *F Subject: FlyBase error report for CG4049 on Mon Mar 17 06:52:58 2003 *F Error report from Steven Bryce (sbryce@hgmp.mrc.ac.uk) *F Gene or accession: CG4049 *F Release: 3 *F DNA error at position 32360. Upstream nucleotides: GAGGAAGTAGAACCATCCAA *F Insertion of length 1: A. *F Corrected sequence: ACCATCCAACCTGGAACCAA. *F Comments: The SNF2 domain of CG4049 is missing a block of key motifs. This *F appears to be due to a base insertion in the genomic sequence (A at position *F 32360 of AE003463). This results in a frameshift and missidentification of the *F appropriate splice junctions producing a deletion of the motifs from the SNF2 *F domain. *F If this base is removed the coding sequence then becomes: *F CDS *F complement(join(30167..30912,30962..31195,31253..31364,31430..32099,32163..3302 *F 6,33171..35512)) *F The translation then contains a full SNF2 motif. *F (Protein window will not accept the aa sequence, so I have coppied it below) *F MEESNPNYDVNQRLQKPEGWPHVNEFHPSMMYPDDPRRYFHNASTDPQHQPQQHPQQQPEQHPEQHPQHPLHSQQQPQQH *F PQQQPQQHPQQQTQQPPQLQPQQHPQAQIQPPIPVPISTPMEGTMEVPVKMPEYCPETSQPVEPDRVINPLAEVAKNTVP *F YGHKRKNSAGEDQAANKKLSMAAAEEKKNSHLRKNIRDVMNENNLDTTTLAAQREESERLARVAGQQKSMREIQKQVVHK *F QIFRILQLDESEGIESCAVANPVEHHPPVDFPEEEIASDTFDDSNSSSLSGGSIEDVLHKGASVQPSEVVTIDDSSDDDC *F ILLSEEEEEEDDEDLNESDDATNSGMHVKDIYNVPDENGQVVVNMAHPEGEETLYLAPQIAKVIKPHQIGGVRFLYDNII *F ESTRRYNKSSGFGCILAHSMGLGKTLQVVSFCDIFLRHTSAKTVLCVMPINTLQNWLSEFNMWIPRYSTDSNVRPRNFDI *F FVLNDQQKTLTARAKVILNWVHDGGVLLIGYELFRLLALKLVKTRKRKGSVIRPDGMDSSSDLMNLVYEALVKPGPDLVI *F CDEGHRIKNSHAGISLALKEIRTRRRIVLTGYPLQNNLLEYWCMVDFVRPNYLGTRTEFCNMFERPIQNGQCVDSTPDDI *F KLMRYRAHVLHSLLLGFVQRRSHTVLQLTLPQKYEYVILVKMTAFQRKLYDTFMTDVVRTKAFPNPLKAFAVCCKIWNHP *F DVLYNFLKKCETDLDLEIDEEVTKGAATPIVEPSADSSLSLASPLEKKINGSGDPINSIETPSFYDEKPEPLNYGSFGSE *F GKNNYWMDSSILPKPGCVEVIKQTDTNMSSNFESITGSSEIVDLDTNEIKTVETTIQAPCSNNQLDNGCNAGKPSEWNAA *F GSKNSSGVAAAEPFKKLLKSKQRNEEFSCSWAVDLMKNYVSGLISNSPKMEIFFCILKESLNLGDRILLFSQSLLTLNLL *F EVYLKSSYVPGSNQLWTKNSSYFRKYAQLPYGMALTLYNFGSRLDGSTSSQERERLVNEFNANSNVKLFLISTRAGSLGI *F NLTGANRVIIFDASWNPCHDTQAVYRIYYGQTKPCFVYRIVMDRCLEKKIYDRQIKKQGMSDRIVDECNPEAHLSMKDIT *F NLCQDYDSDEDTVEEVNKSTGDLSKPGSPSEEGSKPGSANMSPNASNKSNNGPTKPLDDQNTPKSPNGASHSPIKTEKND *F GQEHAVHVDGIINTILDMHRHCVTKEPFMHESLLVDQKDRKLSQAEKRQAHRSYELEKKAAVNQRFTYAPAKMNYKIVNN *F DGTVITKPMNQQIKTNTSSSTGGRSTRWIPADVWQRQGMTAQEMTLPLDVVIPTNSADKANIILKAGQRVMVLKSAKGIY *F MQLDSGKIIAIRTTAKTEPEKTNKDDVIDITDDGDTDTTKPQSPEEDVKDLTADDEPRVVKNKESNPATTSRPEMPAKDR *F KSTENVQHFKSQTQPNTTLATTTNQHQLPSDLTYSQPQQSHQQPQQQASQQQPAQQPPPPAYKHQNHLPAAGEGYSNANS *F IQPNVPLPPHPGAPNSSEAPPPADSSYFQPKPFQHTYPPQYYDYYDNKHKSAPPGSGYHINNYTSYGNLNFAPYHSNLHP *F PASYIGAPSSYVNSNVFPRQHWSSVVNSSPASSESMRQPSYIGSTYPVNEWSSQ # *U FBrf0157248 *a Sackerson *b C. *t 2003.3.7 *T personal communication to FlyBase *u FlyBase error report for CG2328 on Fri Mar 7 09:16:47 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 7 Mar 2003 09:16:47 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: csackerson@iona.edu *F Subject: FlyBase error report for CG2328 on Fri Mar 7 09:16:47 2003 *F Error report from Charles Sackerson (csackerson@iona.edu) *F Gene or accession: CG2328 *F Release: 3 *F Gene annotation error *F Gene CG2328 has incorrect exon/intron structure or translation start site. *F Comments: The gene model for eve extends too far 5'. The reason for this is *F that the evidence used includes community sequence data from 5' regulatory *F regions \- NOT within the transcription unit. # *U FBrf0157249 *a Duffy *b J.B. *t 2003.3.6 *T personal communication to FlyBase *u FlyBase error report for CG12199 on Thu Mar 6 11:14:56 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 6 Mar 2003 11:14:56 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: jduffy@bio.indiana.edu *F Subject: FlyBase error report for CG12199 on Thu Mar 6 11:14:56 2003 *F Error report from JB Duffy (jduffy@bio.indiana.edu) *F Gene or accession: CG12199 *F Release: 3 *F cDNA or EST error *F Comments: We have sequenced a cDNA (LD12728) for this locus. This has been *F submitted to Genbank and the accession number AY195738 has been assigned. This *F sequence does confirm the large size of the gene linking the tow halfs \- 5' *F and 3' ests. Based on the sequence similarity of this gene to other members *F of the kekkon family (kek1, kek2, kek3, and kek4) we have used the name *F kekkon5 for this gene. # *U FBrf0157250 *a Murray *b M. *t 2002.12.6 *T personal communication to FlyBase *u FlyBase error report for CG8874 on Fri Dec 6 04:38:21 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 6 Dec 2002 04:38:21 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: mjm55@mole.bio.cam.ac.uk *F Subject: FlyBase error report for CG8874 on Fri Dec 6 04:38:21 2002 *F Error report from Michael Murray (mjm55@mole.bio.cam.ac.uk) *F Gene or accession: CG8874 *F Release: 3 *F Gene annotation error *F Gene CG8874 has incorrect exon/intron structure or translation start site. *F Comments: Hi, There is an inconsistency in the BDGP sequences. *F Transcript CG8874-RA has a small intron *F gtgcccaagatccagaagaaatcaaaggccatccgcaatacattccgctccaagttgctcaatttccagttgaag *F which is not present in the final sequence for BcDNA:RH14840. *F My sequencing of RH14840, confirms that the intron is an error. *F thanks, *F Mike Murray # *U FBrf0157251 *a Levis *b R. *t 2002.12.17 *T personal communication to FlyBase *u FlyBase error report for CG9403 on Tue Dec 17 16:01:27 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 17 Dec 2002 16:01:27 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG9403 on Tue Dec 17 16:01:27 2002 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG9403 *F Release: 3 *F cDNA or EST error *F Comments: Liu and Montell have sequenced a full length cDNA for the jing gene *F (GenBank accession AF356082) that has its 5' end 3.2 kb upstream of the *F annotated 5' end of the jing gene in the current version of the scaffold *F segment (AE003789.3) available from GenBank. Nucleotides 24-412 of the cDNA *F sequence align perfectly with AE003789.3 positions 275251-275639. This *F appears to be an upstream exon that is not annotated in the current genomic *F sequence record. The current annotations of the genomic sequence place the 5' *F end of the jing gene at AE003789.3 position 278520. # *U FBrf0157252 *a Gilliland *b B. *t 2003.1.7 *T personal communication to FlyBase *u FlyBase error report for CG1763 on Tue Jan 7 14:49:36 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 7 Jan 2003 14:49:36 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: billg@ucdavis.edu *F Subject: FlyBase error report for CG1763 on Tue Jan 7 14:49:36 2003 *F Error report from Bill Gilliland (billg@ucdavis.edu) *F Gene or accession: CG1763 *F Release: 2 *F Gene annotation error *F Gene CG1763 has incorrect exon/intron structure or translation start site. *F Comments: The listed intron/exon structure (Transcript CT5198) does not match *F the supporting cDNA clone (SD02282). Here is the sequence with predicted *F features, based on an alignment of the full-length cDNA with the genomic *F region (>CG1763_extend2000 transcript_highlighted:CT5198 upstream_of_gene:2000). *F LOCUS FIXED MEL 8950 BP DS-DNA SYN 07-JAN-2003 *F DEFINITION \- *F ACCESSION \- *F KEYWORDS \- *F SOURCE \- *F FEATURES Location/Qualifiers *F 3'UTR 2001..2106 *F exon 2107..2223 *F intron 2224..2287 *F exon 2288..2482 *F intron 2483..2552 *F exon 2553..3087 *F intron 3088..3221 *F exon 3222..3367 *F intron 3368..5488 *F exon 5489..5679 *F intron 5680..5742 *F exon 5743..6453 *F intron 6454..6532 *F exon 6533..6638 *F 3'UTR 6639..6934 *F BASE COUNT 2428 A 2118 C 1928 G 2476 T 0 OTHER *F ORIGIN \- *F 1 ATTTAATTCG AAATAAGTTT GGGTATTATT AGCTTATAAT ATTCAAAAGT GTATTTTGCA *F 61 ATTGGAACGA ATTTGGAATC CCGCTATTTT CGGATTCATG CAGTTCCCAT TATATTTTAT *F 121 TCGGTATTGG AATCCCGCTA TTTTCGCATT CATGCAGTTC CCACTATATT TTATTCGGTA *F 181 TTTTCGCAGT CATTAGTGTG GCCAAAACTC GAAAATATCA AGGGCTAGAG CAGCTAGATG *F 241 TTGCGGCATT CGCGGCCTGG TCACACTAAG CTAGGGCTAC TTTTTATATC ATAAGTCGAG *F 301 CGTTTAGGTA AGCGAAACAA AAAGAGCTTT AGTTAGAGGT ATTAGTTTGG TTGCTATTAT *F 361 TTCTAAATTG AAAACTTCAC TTCCTTTGAT CCAAAAGACA CTGAAAAGAC ACGTTTGCAG *F 421 TTGAGTTCAT TATTTTCTGG TATACATACA CACTGATTAC TCATTCAATT GGCATTTTGC *F 481 TCTTGTTTTT CTCCGATTGC CTTGCACTCG CATAAATTTA ACACAAAAAA GAAGTTCGGG *F 541 GTGTTTAGAA TCCATCCACT TGGTACAGTT CCCATCGAGC TGGTGAGTAC TCCGCGCAGT *F 601 GCAACGTATA CACACTTGAA CTCGAATTCT GGCAGCAAAA TGGTGGTGAC CTATTAATAA *F 661 CACCCGCTCA ATTTTCCCTC GCTTTTCCAT TCAGTCTCCA ACTAGCTGCA AGTGAGTACG *F 721 TTACTTCCGA GATCCTCTCC AAAAAAATAA AACATAAATC CAAATCCAAC CAGTGTAGCA *F 781 TCCATTGAAT TAGATTTTCC GACACGTTGC GCCTATGACA ATCCTAATTC CATCCTCTTC *F 841 TCTTTCGTTT AGGTAGCTCG GATGGCCATC GAAAGGGTAA ATTGGTGTTA CATATAGCTT *F 901 TAGAGATCGT TTCGAATCAC ATTGATAATC GTTCGAAACG TTCTCCGAAG CAAAATCAAG *F 961 CAAGAGTAAC GATTTCCGCA TAGTCGAAAA TGTTTAAGTT GAATTGTCTT ACGGACAGTG *F 1021 AGATGAGTAC GACTATTTGG AAATCACAAA CGAATTGTTT TCATGGTTGA CGCGCTTGTC *F 1081 AAGCTACAAA ACAAAATGAA TGATATACAA TATACAATAT ACAATATGCA ATACAATACA *F 1141 ATACAAGACA AAAAAATGTG TCTTGGAACG CAACATTGTA CAAGTCGCAA TGCAAACTGA *F 1201 AGTCTTAAAA GACGTGTAAA ATGTTGCAAA TTAAGCAAAT ATATATGCAT ATATGGGTAA *F 1261 CGTTTTACGC GCCTTAACCA GTCAAAATAC AAAATAAATT GGTAAATTTC ATATAACTAG *F 1321 TGAAATGTTA TACGAAACTT AACAATTGCC AAATAATACA GATCGATTTA GAGCGAGATG *F 1381 ACAATAGAGA GGCGATCTCT CTCGTATACG AGTCTTGAAA AGAAAGAGAA GGCGAACGGT *F 1441 GCTGGCTTAG AGAGAGATGG CAATACTAAT TAACTGCAAA TACATTTCCG CCATTTTGTT *F 1501 GGCGCTAAAA GTAACGGAAA TTCGAGATGC TTTTAGGGCT GCCACCTTGG TTTCCAGGGT *F 1561 GACCAGAACT GACACTTAAA TTACATATGA CAAATAAAGA CTTATCTGCT ATTGCAAATA *F 1621 CTGTTTTAAG CGGCTTTTTT TCCATCAAAT TTGTTAGTTC TTGTTCCTGT TTTAATAAGT *F 1681 TGGCAATACC GATCCTCCGC ACTTGCTATG TGTGTCTATA ATCATATAGG GTGGCAGCGC *F 1741 AGGCCAGGGC TGGCAAGGGT GGCGCATTAA CAGAGTTCTG TAATGTTATG CTCATGAACA *F 1801 ATAGCGATGC CATTGTGCAC GATATGTTTG CAAAACATGT TAAGTTGTGT GCAGTATTTT *F 1861 AGCAAGATTA AGTTAAATTA GAGATGGCCC CTACAACATA CACATACCAA AAGCGAGGTC *F 1921 ACAAGCCACA ATGTAAATAT CCATGACACC AACCCAGATC GTTCTTAGTG TTATTACCGC *F 1981 GACGGTCACA CTGCCAAAAT CAATTTGAAA TGCAAAGGTC GCTTGGCTTC GTCAAAAAAG *F 2041 TAAAATAATT ACGGTGAATG CAAGCCAATT GTGCATTATT CAAACAACTT CAATTCTTCA *F 2101 ATCTGCATGG AGGGCGCCAA ATTAAGCGCA GTTCGGATTG CGGTCCGCGA GGCGCCGTAC *F 2161 CGCCAGTTCT TGGGGCGTCG GGAGCCCAGC GTCGTCCAGT TTCCGCCATG GAGCGACGGA *F 2221 AAGGTATGTG CCCCACCATT GGCCATGTGC ATGTCTAACG GTATATGACC GCCATCTCGC *F 2281 TTTGTAGTCG TTAATAGTGG ATCAGAATGA ATTCCACTTC GATCACGCCT TTCCCGCGAC *F 2341 CATCAGCCAG GATGAGATGT ACCAGGCGCT GATCTTGCCG CTGGTGGACA AGCTGCTCGA *F 2401 GGGATTCCAG TGCACTGCAC TCGCCTACGG CCAGACGGGA ACGGGCAAGA GCTACTCAAT *F 2461 GGGCATGACA CCTCCGGGAG AGGTGGGTTT TTCGTTTAGC CATCAGTGGC TTAAATCTTT *F 2521 CCCACTGATC TATCGCTTCT ATTCCTTTAC AGATACTGCC CGAGCACCTG GGTATTCTGC *F 2581 CTCGCGCCCT GGGCGACATT TTTGAGCGCG TGACCGCCCG GCAGGAGAAC AACAAGGATG *F 2641 CGATTCAGGT GTACGCCTCC TTCATAGAGA TCTACAATGA GAAACCCTTC GATCTGCTGG *F 2701 GCTCCACGCC ACATATGCCC ATGGTGGCGG CGCGTTGCCA GCGATGCACC TGCCTTCCTT *F 2761 TGCACAGCCA GGCGGATCTG CATCACATCT TGGAGCTAGG CACTCGCAAT CGACGCGTTC *F 2821 GTCCCACCAA TATGAATTCC AATAGTTCGC GATCCCATGC CATAGTCACC ATTCACGTGA *F 2881 AGAGTAAAAC CCATCACTCG CGGATGAATA TTGTGGATCT GGCCGGTTCA GAAGGCGTGC *F 2941 GGCGAACTGG GCACGAGGGC GTGGCCAGGC AGGAGGGCGT CAACATCAAT CTGGGCCTGT *F 3001 TGAGCATCAA CAAGGTGGTG ATGTCCATGG CGGCGGGCCA CACAGTGATA CCATACCGCG *F 3061 ACAGCGTCCT TACCACAGTT CTGCAGGGTA AGTGCTACGT TTTGGATCGT CTCATGCTCA *F 3121 ATTAGTTAGT GGACCATTTG GGTTCAAGCC TTTATTGATT AGCGACTTAG TGACTACTAT *F 3181 AAATATTGAC TGCAACTTCT TGCCGTTTTC ATTCTTTGCA GCCTCGCTAA CCGCGCAGTC *F 3241 GTATCTGACC TTTCTGGCCT GCATCAGTCC GCATCAATGC GATCTCAGCG AGACGTTGTC *F 3301 CACCCTGCGT TTTGGCACCA GTGCCAAGAA GCTTCGGCTG AATCCGATGC AAGTGGCGCG *F 3361 CCAGAAGGTG AGCCATCTAT ATGTAATTAA AGTCCACACA GTCGAGGATC ACCTCGCGTA *F 3421 TACGCTGTTT AAATGTAGCC TCGTTGCCCG CGCCCAGGAA ACCAGCTCCA TGGAGATCCG *F 3481 TAGTGGCCCC CGCCTCGATG CGCTCGCTAA TTGCCTGAAG GTCGGGCACA TCCTGACCCT *F 3541 GCATCGTTAC AATGGGTAGA ACCATCATGG CGAGAAGCAG GCCCACAGCC GCTTTGGTAG *F 3601 CCACCTGTTC ATCGAATGCA GGACGGGGAA AAAGTTGCTC CACCCGCTCG CCTAGTCTGT *F 3661 TGCGAACACA ACAATACAAT AGTTGAAGCT CTAGGATTCT TATACTTTTA CTTACCTAAT *F 3721 CAGCTGCAGG CCCAACTCTT CATAGTAATC CTCGAGCATA TTTTCGAGGT GTCGCTGCCG *F 3781 GAATCGCCGC GAAGTGCAAG TGAAGAGGAA GTAAGCTAGA TCGGTGACCG GCGAGGCATA *F 3841 TCGCATCAGT TGCCAGTCGA TCAGGCGCAC GTCCTCGAGC TCGCCCCGCT CTGTACTCTT *F 3901 GTAGAGAATG TTGTTATTCC AGCAATCGCC GTGGCAAATA ACAGCGAAGG GTTCGCAATT *F 3961 GAATCCGCTG ACCAAAGGCA GCAGCAGCTC AAAGTACGAT CCTCTGGCGA AGTACGCCTC *F 4021 CAGACGAACT CTGTATGCAT CATCCGCCGG TGCGAGCAGT GCGGATAGGG CGCTTTCCTT *F 4081 CAGATTCTCG AAGTAGACCC CCAAGGCATG ATCATCGCGT CGCTGCTCAA AGATATCCAC *F 4141 CAGCTGCTGC AGTTGTTGCA TTTTCTCGGG CAACTGCCGT TTCCCGGCCA ATGATATAGC *F 4201 ATGTAATTTG GCATAGGTGA GCATTACGCG ACGCACATGC TCGACGGATA GGTCGAGAAA *F 4261 GCGATTGTGC AGTGAGAAGC CGGCACAAGA CAGATCCTCC AGCACGATGC ACTCGTTGGG *F 4321 CTCATCCTGG CGAGTTCCGA AACAGAGCGC GTGCTGCCGG AAACGATCAT CTCCGATTAT *F 4381 CTTCCACTTA TCCTGAATTA ACGCAGTCAG CGGCAGAAAG ACCTCATATG CTGCCGTCTC *F 4441 GCGCAGGAAA CATGGTCGTG CAAAGAATTG CTTGCGGCGC ACGCGATTCT GTGGCGGCAG *F 4501 TTTAACCACC AGGCTGCGTT TCGAATCGGA TGCCGCCTGT AGGCGCCACA CCACGCCCAA *F 4561 ATAGTTGTCG CCCTTGGCAC TCGCCCTTTC CAGACGCAGT TCCGGATTGG CGCCCAGCTC *F 4621 TGGCCACAGC TGGCTGACCA GTTGGCGAAG GAACTGTGTA ACCTGCTCGT TGGGCAGATC *F 4681 TTCGTCTGGC TTGGCTGTGT CCTCTTGTTG CTCTACAGGA CTCTCCATCC TGTCTGGGTG *F 4741 CTCTCTATTC ACCTCCCCAT TTCGTTCATC TTCGGTGTCT TCATGTGGAT CTGGTTCGGC *F 4801 TTCCTTCTGA TCAACTTGCT CTGCTGGAGC TGCTAGGTTG CCAGTGAGCT GAATCTCACT *F 4861 CGCAGAGATT TCTGCTAGTT TTTCCCTGTC CGCATTCTCT GCCAGCTCAT CCTTTGATTT *F 4921 CGCCTCAGGC TCATCATCTC CTTCCAACAA CTGATCTCCA AATGTTTGAT TCAACTGGGG *F 4981 AATTTTTTTA TCAAGCTCCT CTATGCCTTT GAGCTCTTGT TTGTCGATCA CTTTCGAATC *F 5041 AATCTCTTTG GCTAGTTGTT TTTCAATGTG CTCCACCTCA GCACCTCTCG GTTCCTCCAA *F 5101 CTCTGCCACC AATTCCTCCT TTTTCACACG ATTATCTTCT AAATCCTGCA CTGCTTCGCT *F 5161 GTTATTTGAG TTCTCCTCCT TTGGTTCCTC AACATCAATT CGTTTAGGTG ACTTCTGATC *F 5221 GACATCTGGA TAACTTTCTG TTGAGTCTGG TGAATTTCCT TGATCGGATG CCACTTCTAT *F 5281 CACTTCCTGA CTTTGGCGAT CTTTCTGCTC CCCCTCCTGC GTCTTCTCAC TGGGCTGTTC *F 5341 CATGGCACAC TACGCCACCG CCGCGAGTGT GGATCGTTTA TATAGAGAAA CCAAGCCGTA *F 5401 CCGAACCGAA CTCATTCCAA CCGCACTGAC CGCAAATCGA ACGATGCAGT TACTCGAGTT *F 5461 TCATCCACCC ATCTTGCTCT GATTGCAGCA ATCGCTGGCC GCACGGACAA CACACGTCTT *F 5521 CCGCCAAGCG CTATGCACCT CGACGGCCAT CAAGTCAAAC GCAGCCAATC ATAATAGCAT *F 5581 AGTGGTTCCA AAATCCAAAT ATAGCACAAC CAAGCCGCTG AGCGCCGTGC TCCATCGAAC *F 5641 TCGCTCCGAA CTTGGCATGA CGCCCAAAGC TAAGAAAAGG TGAGTAACAC GGAATGGACC *F 5701 AAAAGCGAAG ATGATCTAAT CCTCCCATAT CTTCCTCCAT AGGGCTCGCG AGCTATTGGA *F 5761 GCTGGAGGAG ACCACGCTGG AGCTCTCGTC TATACACATT CAGGACAGCA GTCTGAGTCT *F 5821 GTTGGGTTTC CATAGCGATA GCGATAAGGA TAGGCATTTA ATGCCTCCCC CAACAGGGCA *F 5881 AGAGCCAAGG CAAGCCAGCA GCCAGAACTC TACGCTAATG GGCATTGTCG AAGAGACCGA *F 5941 GCCCAAGGAA TCGTCAAAGG TGCAACAGTC AATGGTTGCC CCCACGGTGC CCACAACTGT *F 6001 ACGCTGCCAG CTGTTCAACA CCACCATCAG TCCCATCAGT CTACGGGCAT CCAGCTCTCA *F 6061 GCGAGAACTT AGCGGCATCC AGCCAATGGA GGAGACAGTA GTGGCTTCGC CACAGCAGCC *F 6121 ATGCCTTCGT CGTTCCGTGC GTCTAGCGAG TAGCATGCGT TCGCAGAACT ATGGAGCCAT *F 6181 TCCCAAGGTT ATGAATTTGC GGCGCAGCAC GCGGCTGGCG GGAATCCGGG AACATGCCAC *F 6241 CTCCGTTGTT GTGAAAAACG AGACGGATGC GATACCGCAC CTTCGAAGTA CAGTGCAAAA *F 6301 AAAACGTACG CGAAACGTGA AACCTGCGCC CAAGGCCTGG ATGGCCAATA ATACAAAATG *F 6361 TTTTCTGGAC CTGCTTAACA ATGGAAACGT TAAGCAATTG CAGGAGATTC CAGGGATCGG *F 6421 TCCAAAGTCC GCCTTTAGTT TGGCCTTGCA CAGGTAAGTT ACTTACATAG TTGGCATACC *F 6481 TTTATTTTGC GTTTTACCCA ATAACCCTTA ATCCGTAATC CACATTTTTC AGATCCCGCC *F 6541 TGGGTTGCTT CGAGAATCTT TTTCAAGTCA AATCCCTGCC CATTTGGTCG GGAAATAAAT *F 6601 GGGAACGATT TTGTCAAATT AACTGTCTCG ACACTTGATA CAATTACTAA TTAAATAGCA *F 6661 TTTTAATTCG AATATAGTAT AGTGATTGTT ATTTATGTGG CATATACTTT GATTTTACAA *F 6721 CTATAGTAGG AGTAAAAAAA GGAGCCGGAT ATCCTAAAAC AAACAGTTAT ATATGTGTAT *F 6781 GTGTAATATT TCTCAAGATG TTTACTTATT TTTTGTTTTC GACTATAATT TATTTGATGA *F 6841 TAGCTTAGAC TACCTATTAA ATAAGCGCTA TGTAATCTAA TATTTAATTG CATTTTTTGA *F 6901 TTTAAAGTAA AATAACTTTT AGGTCAATAA CAATAATAAT AGGACTTTTA CACTTTGACA *F 6961 CTTTTTATTC TTGGCATATA ATTTTTAGAC AATATACATA TATATATACA TTGATTATTG *F 7021 AAGTCGTCGA TCTTCACTCT TCGCACTCAC TCAACAGGGG GATTCTATCT ACGAATCCAT *F 7081 CAGTTTCGGC CGCTTCGATT CCAGTTTAGT TAGCACTCTC TCCTGGTTGA TTTGGTATAT *F 7141 ACATACATAC ATATTTGATA TATATATGTA TATAGGTATG TATGATCATA CGATTAGCAT *F 7201 ACGAGATAAG CTGCCAATCC AATCCAAACA CATGGCGCTG GAAATCGGCA TTCCATACAC *F 7261 ATTCCTACTC TCTTTTGGCT GGAAATCTTA TCCGATTTCC CTTCGCTTCA AACATCAAAA *F 7321 TACTCTGTTT GGGTTTCCCG AGCGTTGATT CATTAATCTA GCCATGATAT AACAACCAAA *F 7381 ATAATATCAA GCTATAGAAT ATATTTTTTA AAGAAAAAAA ATCAGTCTGC GGGCATCCAG *F 7441 TTCTCAGCGA GAACTTAGCG GCATCCAGCT AATGGAGGAG ACAGTAGTGG CTTCGCCACA *F 7501 GCAGCCATGC CTTCGTCGTT CCGTACGTCT AGCGGGTAGG ATGCGTTCGC AGAACTATAG *F 7561 AGCCATTCCC AAGGTTATGA ATTTGCGGCG CAGCACGCGG CTGGCGGGAA TCCGGGAACA *F 7621 TGCCACCTCC GTTGTTGTGG AAAACGGGAC GCGCCTTCAA AGTGCAGTCC AAAAGAAACG *F 7681 CACGCGAAAA GTGAAACCTG CGCCCAAGGC CTGGATGGCC AATAATACTA AATGTTTACT *F 7741 GGGCCATCTT AACAATGGAA ACGTTAAGCA ATTGCAGGAG ATTCCAGGGA TCGGTTCAAA *F 7801 GACCGCCTCT ATTTTGGCCG TGCACAGGTC AGTTGACATA CGTTCATTTG CGTTTTACCC *F 7861 AATAACCCTT AATCCTTAAT CCACATTTTT CAGATCCCGC CTGGGTTGCT TCAAGAATCT *F 7921 AATTGAAGTC AAATCCCTGC CCATTTGGTC GGGAAATAAA TGGAAACGAT TTTCTCAAAT *F 7981 TAACTGTCTT GACACTTGAT ACAATTACTA ATTAAATAGC ATTTTAATTC GAATATAGTA *F 8041 TAGTGATTGT TATTTATGTG GCATATACTT TGATTTTACA ACTATAGTAG GAGTAAAAAA *F 8101 AAAGGAGCCG GATATCCTTA AAGAAACAGT TATATATGTG ATTTATTTTT TAAATAGGCT *F 8161 TACCTAGGAT ATATTATATA ATATATTAAA TTAACAAAAC AAGTTGGCAT TTTAAATTAA *F 8221 AGCACAAATT GATTGTTATA AAAGTATTAA ACAAAAAAAA CTAGTCAAAA AAGAAAAACT *F 8281 TTCAAATAAA TGACTTATTA AAAGTGACCA AATAAATAAT ATTCATTAAC TTTTTTTTTC *F 8341 AAATTTAACT CCCTTTCAGC GTTGCCAGAT TGGGTTGCAT GCGGGATTTG ATTATTTTTA *F 8401 TTCACAAATT CTGGGTAGAG GGCCACTTGT GCTGCTCCGG CAGCTGGTCA CACTGTACAG *F 8461 TCGCGCAGTT TGGTTTCTTT GGTGCGAGAA GACGGTTGGT GTTGTTATTT TTTTTTTAGT *F 8521 TCGCTCCGCT GTTTTTAGGT GCTACGCGCT TTATGGCCCG CTCTTAATCT TATCGCAAAT *F 8581 CAAATCAAAC CGCGGCCAAT GAGCACCGTA ATTTCACTTT TTGGCTAACG GTAACGGCAC *F 8641 TTCCGCTCTC CGAATTCGAT ATTCAAACAG TCGCATTCCA TCGAAAAGCG AAACAAGAAG *F 8701 TGGCCGTAAA AATGACCGGT GAAAACAGTG AATCGCCAGC AGAGCCAAAC GCCCACCCAA *F 8761 CAACGGCAAT TGCATCAGCA GCGGGACGTC GAGAATGGGC TCTATCCCAC GATTTCGGAC *F 8821 GGAATATGCC ACATTATTAT CGGATAGAAT ACAGGAAATG TAGGATTTAG GTAAGGGAAT *F 8881 AATAAATTTC TAGTAGTTTT CAAGTCATTT TGGAGAGCAC ATAGTGGTTA ATTCATGTAC *F 8941 CATATTAATG *F // # *U FBrf0157253 *a Berman *b B. *t 2003.1.15 *T personal communication to FlyBase *u FlyBase error report for CG2328 on Wed Jan 15 13:25:08 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 15 Jan 2003 13:25:08 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: benb@fruitfly.org *F Subject: FlyBase error report for CG2328 on Wed Jan 15 13:25:08 2003 *F Error report from Ben Berman (benb@fruitfly.org) *F Gene or accession: CG2328 *F No CGI param release--guessing release using get_release *F Release: 3 *F Gene annotation error *F Gene CG2328 has incorrect exon/intron structure or translation start site. *F Comments: The 5' end of the first exon extends about 2kb past the original *F release 2 transcription start site. This extension was apparently based on *F records in GenBank that correspond to promoter/enhancer sequences located *F \-upstream- of the gene (eve stripe 2 enhancer element). You guys should be *F able to locate the actual transcription start site from the other GenBank *F evidence, or just revert back to the release 2 start site. *F Thanks, ben. # *U FBrf0157254 *a Gronostajski *b R. *t 2003.1.21 *T personal communication to FlyBase *u FlyBase error report for CG2380 on Mon Jan 20 16:06:59 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 20 Jan 2003 16:06:59 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: rgron@buffalo.edu *F Subject: FlyBase error report for CG2380 on Mon Jan 20 16:06:59 2003 *F Error report from Richard Gronostajski (rgron@buffalo.edu) *F Gene or accession: CG2380 *F Release: 3 *F cDNA or EST error *F Gene cDNA sequence: *F >GATGAATTTCACCCTTTTATTGAGGCACTCTTACCGTACGTCAAATCATTTTCCTACTCTTGGTTTAATTTGCAAGCC *F GCCAAACGAAAATATTATAAGAAACATGAAAAACGTATGTCACTGGAAGAAGAAAGACATTGTAAAGATGAACTCCAGA *F ATGAAAAGACTGAGGTGAAGCAAAAGTGGGCTTCCCGTCTTCTTGGAAAACTCAGAAAAGACATTACACAAGAAAGTCG *F AGAGGATTTTGTGCAATCTATAATCGGGAAAAGAAAATCAATTTGTGTTCTCTCAAACCCCGATCAAAAGGGAAAAATG *F AGACGAATTGACTGCTTAAGACAGGCTGACAAAGTATGGCGGCTTGATTTGGTCATGGTTATACTCTTCAAGGCGATTC *F CATTAGAAAGCACAGATGGTGAGCGGTTAGAAAAAAATCCGGAATGTTTACATCCAGGATTATGTGTAAATCCGTACCA *F CATTAACGTGTCTGTTCGGGAATTGGACTTGTACTTGGCCAACTTTATAAATACTCATAATTCAATTAACAATACAAAT *F ACGACTTTCTCAACTACTCCGGGCGTAAGATCGCCACATATAACTATGTATAATCGAAGTAATGATCCAAACAGAAAAG *F ATGAGGTCGCTGACATGAAAAATGATGTCGTTAAACAAAACCCATATAATGGTGTTGTTTGTAATGATATTATTTTAGC *F AACTGGCGTGTTTTCTTCTCAAGAACTGTGGACATTATCTAAAGATTTAATTTTAGATGAATCAAATGATATAAACCTA *F AACTCTAGCCTTATAAAGAGGGAGAACGTTGGAGCTACCTATGAGTGTAATACATATCAAATTAACTCTGAATCCTCAA *F TTTCTGCTGCTCAGAGTCTTGTTCAATCTGGGTCAATTGCGGCTAATCCGATTCCACTTGGATATAGCATTGATTTTAT *F AGATCAGAGGATTACACAATTATCACAAAGTCCATTGCCAC! *F GTACAGAAGGCCCAAACGGCGATTCCCATGATGCTAACAAAATCGATCAAAGTAGCTCACCGCCAATCACGAGCACTG *F CATCAGATAATGGCACAAGTAGTTTTTCTTTGATCGTGAGAGCTACGGATAGCTCTATTGAAGATCCAAAAACTTCACC *F CATTTCTTTGCATCGCTATACCTCTTTAAGTAGTCGGACGCTGGCCCAAGCTCTCCCACAACATAGTTGCTCCTTGCTA *F CATTCGTCTTCCACTAGTCCGACTAATACCTTATATTACCCCCAGCATAATAATACAAGACCATCTCAAACAACTTCCG *F TTGAGGAACAAAATCGTGTTGGCTCCGACAAATATTCAACGGAGCCTCAAGATATTTCGGACTTTGTCACTTATGTTTG *F TCAAGACACCAGTCATACGACAACTATAACAGGATCAGCTGAAAACCATTCATTTCAACACTCCCATACACTCCCACAA *F GGTCATGGTCATTCGCCGCATTTTCAAATTCATCAACAGCTGCGATCTGCAAAATTAACGACTTCACCATCAACTCATT *F ATCACAGTACAATGCTTCCGCCGATGCTGCCACCAATGGCTCGACCTGTAGCTATAATTCGCAGTAGCAGTGATCTAAC *F CTTAGTCCAATCCCCACCAACTTCTTTGCCACTGACAGCCCAATCTACAAGCTTGAACGACAATAGTTGTATAGCGAAA *F CATAGCCTGCCAACGGATAATAGACTTGCAAGTAACCTTGACAACAGAAATAGCCCCAATAACACAGATGTCGTTTCAC *F AACACGAAATGACTGGAACTGCATCTCCTCAACCGCAATCTCACACACCGACATTAGCGAGTCCCCAATCTTATCTGGA *F TCCTGGGCGATGTAAGCTTTTATCAGCTGGCTCAAACATAGGCAGAATTACAACACAAATGTATCCCTCATCAAATAAT *F AGAGAATATTTTAATCACTTTCATTCGCAGCCCACATCGCT! *F TTTGGGATATGCCGGCTCTATTTCTACAATGTCTGGAGTTATAAGTCCAACAGACCTAACACTTTACTCA *F GCTCCAATGGCTGTATCACGTTCAAGTACCAGGACTAGATGGAACGAAGAGGAGCATAATGTAATTCCACAGTCTGCAT *F CTAGTACAAACATGGATAACACCCAAGTTATATTAATGGAAGACTCGACTGGTCGTTATATAGATGAGTATTCTAGTCG *F TGACTATGTGTCATGA *F Protein sequence: *F >DEFHPFIEALLPYVKSFSYSWFNLQAAKRKYYKKHEKRMSLEEERHCKDELQNEKTEVKQKWASRLLGKLRKDITQES *F REDFVQSIIGKRKSICVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKAIPLESTDGERLEKNPECLHPGLCVNPY *F HINVSVRELDLYLANFINTHNSINNTNTTFSTTPGVRSPHITMYNRSNDPNRKDEVADMKNDVVKQNPYNGVVCNDIIL *F ATGVFSSQELWTLSKDLILDESNDINLNSSLIKRENVGATYECNTYQINSESSISAAQSLVQSGSIAANPIPLGYSIDF *F IDQRITQLSQSPLPRTEGPNGDSHDANKIDQSSSPPITSTASDNGTSSFSLIVRATDSSIEDPKTSPISLHRYTSLSSR *F TLAQALPQHSCSLLHSSSTSPTNTLYYPQHNNTRPSQTTSVEEQNRVGSDKYSTEPQDISDFVTYVCQDTSHTTTITGS *F AENHSFQHSHTLPQGHGHSPHFQIHQQLRSAKLTTSPSTHYHSTMLPPMLPPMARPVAIIRSSSDLTLVQSPPTSLPLT *F AQSTSLNDNSCIAKHSLPTDNRLASNLDNRNSPNNTDVVSQHEMTGTASPQPQSHTPTLASPQSYLDPGRCKLLSAGSN *F IGRITTQMYPSSNNREYFNHFHSQPTSLLGYAGSISTMSGVISPTDLTLYSAPMAVSRSSTRTRWNEEEHNVIPQSASS *F TNMDNTQVILMEDSTGRYIDEYSSRDYVS* *F Comments: This gene should be marked as the NfI gene in flys. I corrected the *F annotation and sent it a few versions of flybase ago. It was probably under *F my old e-mail address gronosr@ccf.org. We're still missing the first exon, *F but I've removed the incorrect synaptogambin sequences from the 5' end that *F were in the original predicted gene. I have PCR data for all the sequence *F given. # *U FBrf0157255 *a Rotin *b D. *t 2003.2.14 *T personal communication to FlyBase *u FlyBase error report for CG7555 on Fri Feb 14 12:36:07 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 14 Feb 2003 12:36:07 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: drotin@sickkids.ca *F Subject: FlyBase error report for CG7555 on Fri Feb 14 12:36:07 2003 *F Error report from Daniela Rotin (drotin@sickkids.ca) *F Gene or accession: CG7555 *F Release: 3 *F Missed gene *F Gene cDNA sequence: *F > Drosophila Nedd4 cDNA splice variant of CG7555 gene *F AATAAATCGTGTGTGCGCCAAAACCGTGAAAATCAAGTAAAAAGAACGCA *F AAAAAGGCTGCAAGAATATGAAATTCCTTTTTGACCAACTCGTTTTGAGC *F AGTTAAAACCGCCAAACGCGGCCAATATTCTGATGCTCTGTGTGTTTGTT *F TATTTTATAACCCTCGCAATTTATTGTTCAATTTGGTTTGAGAGGCGTGC *F ACGAAAATTAACACAAACGCGAAACGTAAACAGCGGAAGAAAAATCGGCA *F GTGAATAAAGTCCAAATCGGAGGCAAAACCAAATCCAAATCCCAATCCCA *F ATCGCCATCAAAATAAAGTAAAAATGGCCGAGTCAACAACCACATCGCCA *F TCGGTGACGAGCGAAGATGGCCAAATCCATGGCTGCAATAACAGTGATAA *F CGACTCTGGGGACTCGTGTCACCTGCGAATCGTCGTACTCACCGGTCAGT *F CGCTGGCCAAAAAGGATATATTCGGCGCCAGCGACCCGTATGTCAGAATC *F GATTTGAACACAATCAACGGTGATATTAATATTGACTCTGTTTTGACGAA *F GACCAAAAAGAAGACACTGAATCCAACCTGGAATGAAGAGTTCATATTTA *F GAGTTAAACCGTCTGAGCACAAGCTTGTGTTTCAAGTATTTGACGAGAAT *F CGCCTGACACGCGACGACTTCCTGGGCATGGTGGAGCTGACCCTGGTGAA *F TCTGCCTACCGAGCAGGAAGGTCGCACCATCGGAGAACAAAGCTACACTC *F TGCGCCCGCGCAGCGCCAAATCCCGCATCAAGGGCACACTGCGCATCTAC *F CACGCCTTTATCCGGGAGACGCGGGAGCAGAGCGAGCCATCTAGTGGCAA *F TAGCGATGGCGAGTGGGAGCACGTTGAGGCCACCAATGCCGGTGAGACGT *F CTGCCCAGCCGCACCCCTTTCCTACTGGTGGCCACGATGCCCTACCCGCT *F GGATGGGAAGAACGCCAGGATGCCAACGGGCGCACGTACTATGTGAATCA *F CACGGCGAGGACGACGCAATGGGATAGGCCTACTGTCTTAAACAGCCACA *F GTAGCCAATCCACTGACGACCAGTTGGCCTCGGATTTCCAGAGACGTTTC *F CACATCAGCGTGGACGACACGGAGTCAGGACGTTCGGCGAATGAAGACAC *F CGATCACACAGACAGCCACAATCCCTCTGACATCTCAGCTCCGTCCACAC *F GACGCAATTCCGAGGAAGACAACGCGGCTGTGCCTCCCATGGAACAAAAT *F ACTGGTGGTGAAGAGGAACCCCTGCCACCGCGCTGGTCGATGCAGGTAGC *F CCCCAACGGAAGGACATTCTTCATTGATCATGCGTCCCGGAGGACTACTT *F GGATAGACCCGCGCAACGGACGGGCCAGCCCTATGCCAAATCAAACTCGT *F CGCGTCGAGGACGACCTGGGTCCTTTACCAGAGGGCTGGGAGGAGCGAGT *F ACACACCGACGGACGCGTATTCTATATAGATCATAACACGCGAACTACGC *F AGTGGGAAGATCCTCGCTTGTCCAATCCAAATATTGCCGGTCAGGCTGTG *F CCCTATTCTCGAGATTACAAGCAAAAATACGAGTATTTCAAGAGTCACAT *F TAGAAAGCCTACAAATGTACCAAATAAATTTGAAATACGTATTCGACGTA *F CGTCGATACTGGAGGATTCTTACAGAATTATTAGTTCGGTAACGAAGACC *F GATTTACTAAAGACTAAATTATGGGTAGAGTTTGAAGGAGAAACTGGCTT *F AGATTATGGAGGCCTCGCAAGGGAATGGTTCTATTTATTATCAAAAGAAA *F TGTTTAATCCGTACTACGGACTATTCGAGTACTCGGCCATGGACAACTAC *F ACGCTTCAGATAAACAATGGTAGTGGTTTGTGCAACGAGGAGCATTTAAG *F TTACTTTAAATTCATTGGCCGTATTGCGGGCATGGCTGTGTATCATGGAA *F AGCTGCTAGATGCCTTCTTCATTCGTCCATTCTACAAGATGATGCTGCAG *F AAGCCGATTGACCTAAAGGACATGGAGTCTGTGGACACGGAGTACTACAA *F CTCGCTGATGTGGATTAAGGAGAACGATCCACGCATTCTGGAACTGACTT *F TCTGCCTGGATGAAGACGTTTTTGGCCAGAAGAGTCAGCACGAACTAAAG *F CCCGGCGGTGCCAACATAGACGTGACTAACGAAAACAAGGATGAATACAT *F TAAATTGGTTATCGAATGGCGCTTTGTGGCACGTGTCAAGGAACAGATGT *F CGTCCTTCCTTGATGGTTTTGGATCGATAATTCCTCTGAATCTTATTAAG *F ATTTTCGACGAGCACGAACTGGAGCTACTGATGTGTGGCATACAGAACAT *F CGATGTGAAGGATTGGCGCGAGAATACTCTGTACAAGGGCGACTACCACA *F TGAATCATATTATTATTCAGTGGTTCTGGAGGGCTGTACTTTCCTTCTCC *F AACGAGATGCGTTCTCGTCTGCTGCAATTTGTTACGGGCACTTCACGCGT *F GCCCATGAACGGTTTCAAGGAGCTGTACGGCTCAAATGGCCCCCAGATGT *F TTACTATCGAAAAGTGGGGCACTCCTAATAACTTTCCAAGGGCACATACC *F TGTTTCAATCGCCTGGACCTGCCACCCTATGAAGGCTACCTGCAACTGAA *F GGACAAACTGATCAAGGCCATCGAGGGCAGCCAAGGATTTGCTGGAGTTG *F ATTAACACCGGCAACGGGAGTGCAAGCGAATGGCTTTGGCTGGAGGTGCA *F GCAACAGTTTCTGCAGCGCCAGGAGGAGGAGCAGTTACAACGATTCCACA *F CATCACAAGTACCACAAGATCAACAACAACAACGACTTGAAGAGCAGCAA *F CAGCGAAATCAGCGGCACAACCGCAACCTCATTGCATTCACTTGGAATAG *F CTCCGCCAGCAGATCGTGTACTTATTTGTTCGTTTGCTTTTGTATTATAA *F TCATTTTTGCTAGTTTAGTGTTATTTCGTATGTATTTTAACTAGGCGCCA *F AAACTCACACACTACACATACACCTTAAACAAGCAGAAAAAAGATGTTTA *F CTATCGAAAAGTGGGGCACTCCTAATAACTTTCCAAGGGCA *F Protein sequence: *F > Drosophila Nedd4 protein splice variant of CG7555 gene *F MAESTTTSPSVTSEDGQIHGCNNSDNDSGDSCHLRIVVLTGQSLAKKDIF *F GASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHK *F LVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRSAKS *F RIKGTLRIYHAFIRETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFP *F TGGHDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQ *F LASDFQRRFHISVDDTESGRSANEDTDHTDSHNPSDISAPSTRRNSEEDN *F AAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGR *F ASPMPNQTRRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLS *F NPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSY *F RIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGL *F FEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFI *F RPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVF *F GQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFG *F SIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQW *F FWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGT *F PNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGFAGVD *F Comments: This is an additional splice variant of Drosophila Nedd4 that has *F not yet been reported for the CG7555 gene. The partial cDNA sequence was *F isolated in our lab from a screen of a Drosophila embryonic expression library *F (using a PY motif as bait). The full-length sequence was obtained by 5'RACE. # *U FBrf0157256 *a Kovalick *b G. *t 2003.2.20 *T personal communication to FlyBase *u FlyBase error report for CG32313 on Thu Feb 20 15:50:03 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 20 Feb 2003 15:50:03 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG32313 on Thu Feb 20 15:50:03 2003 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG32313 *F Release: 3 *F Missed gene *F Gene cDNA sequence: *F >CG32313 *F ttatgcgaactcaaacccatggtcaacaatgtgttctgcatcccaaaacgttgtcggtcgcttgggaacattggacgcc *F atctctcgcaaaatgtttccaggaggagcatatctcaccactaggtatcgcgtttcctgtattctgcctagtccaacgc *F caagtctggtggaggcgttccatatcatggccgtaaacctgtaatactttgtgttctcggcgaagcctcggtaatgaaa *F ccaaattcgaacacattccctaggcaaggcctcccggaaaacgcagacgacttgaaggtgctttgctgatattgggtcc *F gttgcatacaggtattccaagtagttctctgtatatacaccttcattcctgactatttcatctgcgtattctgagcact *F ctgtacagagttcttcgtccagaaccatgggctgattaccgtacttggccctccttcgattgtgagcctccaggattaa *F ggagcgaagacctatgccgattgtctggttgcactcgatggcgattaaaaacaaaataatttttatgctcat *F Protein sequence: *F >CG32313 *F MSIKIILFLIAIECNQTIGIGLRSLILEAHNRRRAKYGNQPMVLDEELCTECSEYADEIVRNEGVYTENYLEYLYATDP *F ISAKHLQVVCVFREALPRECVRIWFHYRGFAENTKYYRFTAMIWNASTRLGVGLGRIQETRYLVVRYAPPGNILREMAS *F NVPKRPTTFWDAEHIVDHGFEFA *F Comments: CG32313 has sequence similarity to SCP/TPX-1/Ag5/PR-1/Sc (SCP/TAPS) *F proteins. However, the SCP/TAPS domain is incomplete. CG32313 is missing the *F N-terminal portion of the domain. A sequence with similarity to this *F N-terminal portion lies upstream of the remaining SCP/TAPS domain within the *F genome. This coding sequence is interrupted by two apparent phase 0 introns. *F The sequences given above are supported by EST evidence (BE975960). # *U FBrf0157257 *a Kovalick *b G. *t 2003.2.21 *T personal communication to FlyBase *u FlyBase error report for CG32679 on Fri Feb 21 11:32:43 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 21 Feb 2003 11:32:43 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: kovalick_g@utpb.edu *F Subject: FlyBase error report for CG32679 on Fri Feb 21 11:32:43 2003 *F Error report from Gae Kovalick (kovalick_g@utpb.edu) *F Gene or accession: CG32679 *F Release: 3 *F Gene annotation error *F Gene CG32679 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG32679 *F ttatcccgaccattgattatagttgtacacctcgttgggcgagcagagattcggatagttggagtttcgtcctgtagtg *F cattccgaggcagcggttcccgccctgtaaaccgggttattcaccacattggtgaccgcgtagttgcaagccagcaggg *F tggcctgcacattgttggcgtccgtaaatcgagcaatcgcacaacccactcggttatttcgctcgttgaccatggtggt *F gaagtggccaattgctggtccaccgcgcatctgatagtcctccatgtcgccactggtcgcatcccgattctcatcgaac *F caggcggcgatccgttggcgcaggaagaccgccacatccacacttcgggtgaagagaatgctcagattctggccggcat *F agcga *F tacgtgttcgtgttgcggcattcgtcgtgcgccatgcggcactgcagcgcattgtaggcggccagttgggccagtgtcg *F tgtcccaggacattgtggccatttggacggcactggggaagccactaactccaccgccagcgatcaggttgcggcgttc *F gttgtgcagctggagaatcagcgggatgtgggcatccacttggacaaactcgccgctgcaaccgctcacaaagcgaccg *F ctgttttggcaggccacgtgtcttccactgggacacagttctggatcgcaatagttttgtgcgaaagtaaaagtgaaaa *F tgataaggaccaaatataaaaggaaaatccagcagggagatcgtgacat *F Protein sequence: *F >CG32679 *F MSRSPCWIFLLYLVLIIFTFTFAQNYCDPELCPSGRHVACQNSGRFVSGCSGEFVQVDAHIPLILQLHNERRNLIAGGG *F VSGFPSAVQMATMSWDTTLAQ *F LAAYNALQCRMAHDECRNTNTYRYAGQNLSILFTRSVDVAVFLRQRIAAWFDENRDATSGDMEDYQMRGGPAIGHFTTM *F VNERNNRVGCAIARFTDANNV *F QATLLACNYAVTNVVNNPVYRAGTAASECTTGRNSNYPNLCSPNEVYNYNQWSG *F Comments: CG32679 has sequence similarity to SCP/Tpx-1/Ag5/PR-1/Sc (SCP/TAPS) *F proteins. In vespid wasps, Ag5 proteins have a sequence containing four *F conserved cysteines in a domain that is N-terminal to the SCP/TAPS domain. *F The four conserved cysteines participate in disulfide bonding. A similar *F domain with four conserved cysteines is also present in many Drosophila *F SCP/TAPS proteins. These include Agr, Agr2, CG9400, CG6628, and CG5106. A *F sequence encoding a similar N-terminal domain lies upstream of the CG32679 *F SCP/TAPS coding sequence. The two sequences are interrupted by an apparent *F phase 0 intron. A phase 0 intron with a similar location is also present in *F Agr, CG6628, CG5106 and other SCP/TAPS genes. # *U FBrf0157275 *a Gilmour *b D.S. *t 2003.4.18 *T personal communication to FlyBase *u FlyBase error report for CG9984 on Fri Apr 18 07:07:36 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 18 Apr 2003 07:07:36 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: dsg11@psu.edu *F Subject: FlyBase error report for CG9984 on Fri Apr 18 07:07:36 2003 *F Error report from David S. Gilmour (dsg11@psu.edu) *F Gene or accession: CG9984 *F Release: 3 *F Missed gene *F Comments: We have raised antibody against the N-terminal part of the gene *F product referred to as CG9984-PA, and this antibody detects a single protein *F on a immunoblot of Drosophila nuclear proteins that is approximately 60 kD. *F This size is close to the predicted size, thus verifying that CG9984-PA *F encodes a real protein. The protein goes by the name of NELF-D, and it is *F involved in promoter proximal pausing on the hsp70 heat shock gene in *F Drosophila. A manuscript describing the protein is in press in Genes and *F Development (v.17, Wu et al). # *U FBrf0157276 *a Bailey *b A. *t 2003.4.11 *T personal communication to FlyBase *u FlyBase error report for CG4241 on Fri Apr 11 11:51:50 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 11 Apr 2003 11:51:50 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: adina@fruitfly.org *F Subject: FlyBase error report for CG4241 on Fri Apr 11 11:51:50 2003 *F Error report from Adina Bailey (adina@fruitfly.org) *F Gene or accession: CG4241 *F Release: 3 *F Missed gene *F Comments: *F CG4241 appears to correspond to the gene, alternative testis transcripts (att) *F described by Madigan et al., Mol. Cell. Biol., August 1996, 4222-4230. The *F sequences reported in this reference don't seem to have been submitted to *F GenBank # *U FBrf0157277 *a Levis *b R. *t 2003.3.14 *T personal communication to FlyBase *u FlyBase error report for CG4551 on Fri Mar 14 08:24:47 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 14 Mar 2003 08:24:47 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG4551 on Fri Mar 14 08:24:47 2003 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG4551 *F Release: 3 *F cDNA or EST error *F Comments: There is an annotation note that 'EST data suggest additional 5' *F exon(s) (that may lie as far as 24kb upstream). ' I assume that the curator *F was referring to LD02884-5prime. The BDGP report on DGC LD02884 indicates *F that LD02884-3prime gives a BLAST hit to CG4551. It seems likely to me that *F LD02884-5prime is an EST for a 5' exon for CG4551. There are two P-element *F insertions near the 5' end of LD02884, which is further evidence that this is *F the 5' end of a gene. I haven't checked Apollo to look at all of the EST *F data. I'm writing to bring your attention to this with the hope that one of *F the curators will reexamine the evidence. # *U FBrf0157278 *a Davis *b T. *t 2003.2.20 *T personal communication to FlyBase *u FlyBase error report for CG15283 on Thu Feb 20 05:43:50 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 20 Feb 2003 05:43:50 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@cardiff.ac.uk *F Subject: FlyBase error report for CG15283 on Thu Feb 20 05:43:50 2003 *F Error report from terence davis (davist2@cardiff.ac.uk) *F Gene or accession: CG15283 *F Release: 3 *F Missed gene *F Comments: I have found an ORF in the Drosophila pseudoobscura genome with high *F homology to CG15283. *F D.pseudoobscura Contig2322 from position: 5358..5071 (Complementary strand). *F This makes a 95aa protein with 62/87 (71%) identity; 74/87 (85%) similarity *F with CG15283-PA. # *U FBrf0157279 *a Davis *b T. *t 2003.2.20 *T personal communication to FlyBase *u FlyBase error report for CG15284 on Thu Feb 20 04:02:58 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 20 Feb 2003 04:02:58 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: davist2@cardiff.ac.uk *F Subject: FlyBase error report for CG15284 on Thu Feb 20 04:02:58 2003 *F Error report from terence davis (davist2@cardiff.ac.uk) *F Gene or accession: CG15284 *F Release: 3 *F Missed gene *F Comments: I have found a putative gene in the Drosophila pseudoobscura genome *F that has very high homology to gene CG15284. *F D.pseudoobscura Contig2201 from position: 19925..19668; 19597..19427 *F (complementary strand); intron 70bp. This makes a 142 aa protein with 134/142 *F (94%) aa identity; 139/142 (97%) similarity with CG15284-PA. The *F D.pseudoobscura protein is also a putative cystine-kont cytokine and has all *F the conserved cysteine residues. # *U FBrf0157280 *a Levis *b R. *t 2002.12.19 *T personal communication to FlyBase *u FlyBase error report for CG1250 on Thu Dec 19 13:56:44 2002. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 19 Dec 2002 13:56:44 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: levis@ciwemb.edu *F Subject: FlyBase error report for CG1250 on Thu Dec 19 13:56:44 2002 *F Error report from Robert Levis (levis@ciwemb.edu) *F Gene or accession: CG1250 *F Release: 3 *F Gene annotation error *F P element P{lacW}l(3)j13C8j13C8 hits gene CG1250. *F Comments: Based on the flanking sequence determined by BDGP, the P-element *F insertion P{lacW}l(3)j13C8j13C8 (aka l(3)j13C8) is inserted at base 460 of *F the 5' UTR of the sec23 (= CG1250) gene, according to the current gene model *F (scaffold segment accession AE003602.3). # *U FBrf0157288 *a Haynes *b S. *t 2003.2.24 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Feb 10 10:23:08 2003 *F To: alal@usuhs.mil *F Subject: Helping FlyBase: ADRC-10414 *F Dear Ashish, *F We are currently curating the abstracts for the upcoming 44th *F (Chicago) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F You mention gene symbols that are new to FlyBase, Scpr-A, Scpr-B and *F Scpr-C. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From shaynes@usuhs.mil Mon Feb 24 22:38:24 2003 *F To: *F Subject: Re: Helping FlyBase: ADRC-10414 *F Dear Rachel, *F I'm sorry for the delay in responding to your request; my postdoc, Ashish, was *F not sure how to answer you and just recently forwarded your message to me. *F The corresponding CG numbers for these genes (which should start with *F lowercase) are: *F scpr-A = CG5207 *F scpr-B = CG17210 *F scpr-C = CG5106 *F Please let me know if you require further information. *F Regards, *F Sue Haynes # *U FBrf0157289 *a Perrimon *b N. *t 2003.3.13 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Mar 13 17:44:42 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{FRT(whs)} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Norbert Perrimon, Harvard U. *F P{FRT(whs)}101 is a homozygous and hemizygous viable and fertile *F insertion at 14AB. *F P{FRT(whs)}G13 is a homozygous viable and fertile insertion at 42B. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157290 *a Yanicostas *b C. *c N. *d Raich *t 2003.2.3 *T personal communication to FlyBase *u phtf expression in Drosophila testis [text description]. *F X-Sender:raich@mail.cochin.inserm.fr *F Date: Mon, 3 Feb 2003 14:03:34 \+0100 *F To: flybase-updates@morgan.harvard.edu *F Subject: personal communication to fly base *F We are writing to you about the phtf gene (Gene phtf : FBgn0028579). *F we would like to add an expression data about phtf expression in drosophila *F testis. We have shown that the level of accumulation of d-phtf transcript *F was almost imperceptible in germ line-les compared to wild-type males *F indicating that expression of the d-phtf gene in Drosophila is essentially *F restricted to the male germinal cells.The canton S stock of Drosophila *F melanogaster was used. Germ line-less flies were obtained by crossing *F tud1bw sp/tud1 bw sp virgin females with wild-type males of the Canton S *F stock. *F The figure represents a northern blot of RNAs isolated from wild-type (wt) *F and germ line-less tudor (Tu) adult male flies probed with a d-phtf probe. *F Levels of RNA were verified by hybridization with a control probe specific *F for the rpL17A gene *F Citation from C. Yanicostas and N. Raich # *U FBrf0157291 *a Michelson *b A. *t 2003.3.18 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Tue Mar 18 01:50:13 2003 *F Subject: Components of stocks from the Michelson lab *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Components of stocks from the Michelson lab *F These insertions are described, named and sequence-mapped in FBrf0152364, *F Halfon et al., genesis 2002. The cytology below is inferred from the *F insertion site given in the publication. *F Enhanced GFP insertions *F P{UAS-2xEGFP}AH2 28F3-5 *F P{UAS-2xEGFP}AH3 70D *F Enhanced YFP insertions *F P{UAS-2xEYFP}AX 10B *F P{UAS-2xEYFP}AH2 58D8 *F P{UAS-2xEYFP}AH3 68C *F These insertions are described but not named in FBrf0152364, Halfon et al., *F genesis 2002. They were generated by hopping an unnamed 2nd chromosome *F insertion of P{GAL4-twi.G} described in FBrf0059139, Greig and Akam, Nature *F 1993. They map to the chromosome indicated. The insertion identifiers are *F those used in the Bloomington stock list. *F Twist-GAL4 insertions *F P{GAL4-twi.G}2.2 2- *F P{GAL4-twi.G}2.3 3- *F These balancers expressing enhanced GFP from embryonic stage 8 through adult *F are described in FBrf0152364, Halfon et al., genesis 2002. They were *F generated by hopping P{UAS-2xEGFP}AH2 into CyO and TM3 chromosomes. The *F symbol used by the donor and the balancer short genotype used in the *F Bloomington stock list are shown. *F CTG CyO, P{w+mC=GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2 *F TTG TM3, P{w+mC=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb1 Ser1 *F An additional balancer was mentioned by not further described in FBrf0152364, *F Halfon et al., genesis 2002. It carries an otherwise undescribed insertion of *F P{UAS-2xEGFP} on chromosome 1 that we have called AX, in keeping with other *F names in this set. The identify of the P{GAL4-twi.G} insertion is our best *F guess. *F FM7c, P{w+mC=GAL4-twi.G}108.4, P{UAS-2xEGFP}AX # *U FBrf0157292 *a Yasuda *b G. *c B. *d Wakimoto *t 2003.3.18 *T personal communication to FlyBase *u *F From GYASUDA@seattleu.edu Tue Mar 18 00:06:22 2003 *F To: ''flybase-updates@morgan.harvard.edu'' *F Cc: ''wakimoto@u.washington.edu'' *F Subject: ms(3)k81 gene *F Dear Fly Base Curators, *F We wish to provide information for FlyBase on the identification of the *F ms(3)K81 gene which was reported in our previous publications. The *F gene was mapped through the characterization of deficiency alleles *F generated by excisions of P elements in 97D. These four alleles are *F ms(3)K812 through ms(3)K816 and are described in Yasuda, Schubiger, *F and Wakimoto, 1995. Genetics 140:219-229. *F Existing mutations are strict paternal effect mutations. The mutant *F phenotype was fully rescued by transformation with a fragment *F containing nts# 22690254 through 22692698 (Release 3.1). This 2.4 kb *F fragment spanned from the 2nd exon of the Rb97D gene (CG6354) through *F the 2nd exon of the rough gene (CG6348). The transformation *F construct is a Bgl II fragment into the BamHI site of the pCaSpeR 4 *F vector. The orientation is nt 1-4793 of casper then *F 22690254-22693698 of the K81 region. The transformants were *F initially tested against the ms(3)K812 deficiency allele and have *F subsequently been tested as a trans-heterozygote against the other *F alleles and the larger Df(3R)roXB3. This rescue demonstrated that *F the ms(3)K81 gene is equivalent to CG14251 22691043-22691597. *F Characterization of a series of cDNA clones isolated from a testes-cDNA *F library revealed that there is no evidence of splicing of this *F message. Based on the cDNAs, the transcription start site is placed at *F \-42 and the poly-A tail is initiated 52nt downstream of the translation *F terminator sequence. With this placement, there are only 154bps *F between the Rb97D and K81 transcripts on the 5' end and only 260 bps *F between the K81 and rough transcripts on the 3' end. The only *F significant similarity to this gene in the data base is to a second *F gene in Drosophila, the CG6874 gene. [Reported at the 38th Annual *F Drosophila Research Conference, April 16-20, 1997]. *F The K81 transcript is testes-specific and is first observed in pupal *F stages. The transcript is only observed in the primary spermatocyte *F stages. Sequence analysis of two EMS-induced alleles reveals both *F result in nonsense mutations causing a shortened K81 product. The two *F ems alleles should be designated ms(3)K81Z3416 and ms(3)K81Z5795. *F The reference for the isolation of these alleles should be : Wakimoto, *F Lindsley, and Herrera (either ms in prep or personal communication). *F They both show the paternal-effect lethality characteristic of the *F other alleles. The Z3416 allele is predicted to produce a short 56aa *F polypeptide. The Z5795 allele is predicted to produce a K81 product *F that is missing only the last amino-acid. The C-terminal Glycine is *F found in the predicted CG6874 and in the K81 sequences from 5 other *F species. The sequence from a 6th species is highly conserved in the *F C-terminal region, but I do not yet have sequence information for the *F last 3 amino-acids. *F Please email us if you would like additional information. *F Thank you, *F Glenn Yasuda *F Chairman of Biology *F Assoc. Professor of Biology *F Biology Department, Seattle University *F 900 Broadway, Seattle WA 98122 *F phone: (206) 296-5980 fax: (206)296-5634 *F Barbara Wakimoto *F Professor *F University of Washington *F Department of Biology *F Box 351800 *F Seattle, WA 98195-1800 *F PH (206) 543-0487, 543-0567 *F FAX (206) 543-3041 # *U FBrf0157293 *a Bloomington Drosophila Stock Center *b ?. *t 2003.3.24 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Mar 24 20:21:26 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{GawB}c309 insertion *F P{GawB}c309 is a homozygous viable and fertile second chromosome *F insertion. The insertion location was inferred from the segregation of the *F miniwhite marker from Cy1 in the cross w1118 females x w1/Y; *F In(2L)Cy, In(2R)Cy, Cy1/P{w+mW.hs=GawB}c309; TM6B, Tb1/+ males. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157294 *a Bloomington Drosophila Stock Center *b ?. *t 2003.3.31 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sun Mar 30 03:52:32 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Revised cytological breakpoints for Df(2L)spd and Df(2L)XE-2750 *F Revised cytological breakpoints for Df(2L)spd and Df(2L)XE-2750 *F Df(2L)XE-2750 *F 28A4 present, 28B1,2 doublet absent, 28C1 absent, 28D1,2 doublet *F present. It's also likely that 28C7,8 present, but conservative *F interpretation is 28A5-B1;28C1-9. *F Df(2L)spd *F Though reported in literature as a simple deletion, it is really a *F deficient inversion. The breakpoints are *F 26A6-B1;27E1-3;28B1-3. Inversion breakpoint between 26A6 and 26B1. *F 27E1,2 doublet may be partially deleted. 27E3 definitely deleted. *F Deletion breakpoint between 26B1,2 and B3. 28C1 present. Therefore, *F cytology is 27E1-3;28B1-3;26A6-B1 with the deletion between the first *F two breakpoints. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157295 *a Kozopas *b K. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Mar 31 21:31:41 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-Wnt2.K}5A *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Karen Kozopas (3/03). *F P{UAS-Wnt2.K}5A is a homozygous and hemizygous viable and fertile, X-linked *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157296 *a Zhang *b Y. *c K. *d Broadie *t 2003.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 01 03:23:33 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Broadie insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Yong Zhang and Kendal Broadie, Vanderbilt University (2/03). *F P{UAS-n-syb.eGFP}1 is an X-linked insertion. *F P{UAS-syt.eGFP}1 is a homozygous and hemizygous viable and fertile, *F X-linked insertion. *F P{UAS-n-syb.eGFP}2 and P{UAS-syt.eGFP}2 are homozygous viable and fertile, *F second chromosome insertions. *F P{UAS-n-syb.eGFP}3 and P{UAS-syt.eGFP}3 are homozygous viable and fertile, *F third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157297 *a Zhang *b Y. *c K. *d Broadie *t 2003.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 01 03:27:55 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-Fmr1.Z}3 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Yong Zhang and Kendal Broadie, Vanderbilt University (2/03). *F P{UAS-Fmr1.Z}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157298 *a Golic *b K. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 01 15:21:19 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Golic insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kent Golic, University of Utah (3/03). *F P{70FLP}11 is a second chromosome insertion. *F P{70FLP}23 is a third chromosome insertion. *F P{70FLP}10 is a second chromosome insertion. It is expressed at a high *F level without heat shock. *F P{70I-SceI}2B is a second chromosome insertion. *F P{70I-SceI}4A is a third chromosome insertion. *F P{hs-I-CreI.R}2A is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. P{hs-I-CreI.R}1A is a third chromosome *F insertion. Synonyms: P{70I-CreI}2A and P{70I-CreI}1A. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157299 *a Roote *b J. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 01 15:51:43 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{RS3}CB-0560-3 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by John Roote, University of Cambridge (3/03). *F P{RS3}CB-0560-3 is a Y-linked insertion. In transposition screens, it gave *F a jump frequency of 51%, i.e. 51% of vials gave at least one jump. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157300 *a Bellen *b H. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 01 17:49:56 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{lacW}A3-3-53 *F The following information was provided by Hugo Bellen, Baylor College of *F Medicine (3/03). *F P{lacW}A3-3-53 is a homozygous viable and fertile insertion at 94D. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157301 *a Bellen *b H. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 01 19:25:14 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: dedU9 *F The following information was provided by Hugo Bellen, Baylor College of *F Medicine (3/03). *F dedU9 is a loss-of-function EMS allele. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157302 *a Kozopas *b K. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Mar 31 21:27:13 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Mutations in 45CD *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Karen Kozopas (3/03). *F Five complementation groups were defined by the EMS screen described in *F Kozopas et al. (Genes Dev. 12: 1155--1165, 1998) based on their failure to *F complement Df(2R)11, a deletion with breakpoints 45C6;45E1. The Wnt2 *F alleles were described in the paper. The remaining four complementation *F groups are described here. *F 1. wunen alleles *F wunEMS4, wunEMS23 and wunEMS41 failed to complement wunk09507, *F wunk10201, wunk15909 and Df(2R)Np5. They also failed to complement *F l(2)k16806k16806, which failed to complement wun alleles in Zhang et al. *F (Genetics 143:1231--1241, 1996). *F 2. l(2)45CDa alleles *F l(2)45CDaEMS3 and l(2)45CDaEMS25 failed to complement Df(2R)Np5. *F 3. l(2)03659 allele *F l(2)03659EMS93 failed to complement l(2)0365903659 and Df(2R)Np5. It *F also failed to complement l(2)03659EMS5, which was lost. *F l(2)03659EMS93/l(2)03659EMS5 individuals had low viability, short *F bristles on the head, thorax and scutellum, and downward curving *F wings. They were also sterile. *F 4. l(2)45Da alleles *F l(2)45DaEMS9, l(2)45DaEMS39 and l(2)45DaEMS74 complemented Df(2R)Np5. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157303 *a Kozopas *b K. *t 2003.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Mar 31 21:50:06 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Wnt2RJ and Wnt2I *F The following information about the temperature sensitivity of Wnt2RJ and *F WntI is quoted from a letter to the Bloomington Stock Center from Karen *F Kozopas (3/03). *F The ts nature of Wnt2(RJ) is described in Dev Biol. 2002 Mar *F 15;243(2):312-25. The ts nature of Wnt2(I) has not been published. The ts *F phenotype is different for each allele, affecting the two tissues where *F I've studied the phenotype differently: *F This is the Wnt2 null phenotype in the two tissues: *F Testis phenotype: loss of pigment cells, malformed or absent muscle layer *F Held-out wing phenotype: wings held out at 90 degrees from body, loss or *F misattachment of direct flight muscle (DFM) 52, misattachment of other DFMs *F Allele Wnt2(RJ)/Df(2R)11 phenotype: *F [note that the Wnt2(RJ) alllele has an associated lethal that I haven't *F been able to get rid of by recombination, so I observed the ts phenotype in *F the transheterozygotes] *F Permissive temperature (18 degrees) *F Testis phenotype: wild type *F Held out wing phenotype: wild type *F Nonpermissive temperature (29 degrees) *F Testis phenotype: hypomorphic, loss of some pigment cells but little or no *F muscle loss *F Held out wing phenotype: wings held out at 45 to 90 degrees, loss of DFM *F 52, hypomorphic for misattachment of other DFMs *F Allele Wnt2(I) *F Permissive temperature (18 degrees) *F Testis phenotype: wild type *F Held out wing phenotype: wings are held out, DFMs not studied in detail *F Nonpermissive temperature (29 degrees) *F Testis phenotype: severe loss of pigment cells and muscle, *F indistinguishable from null phenotype *F Held out wing phenotype: wings are held out, DFMs not studied in detail *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157304 *a Deal-Herr *b M. *c K. *d Cook *t 2003.4.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Apr 02 19:23:40 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2R)BSC29 *F Isolation and Characterization of Df(2R)BSC29 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center *F Df(2R)BSC29 was isolated as a P transposase-induced male recombination *F event involoving P{PZ}l(2)0365903659 and P{lacW}l(2)k09501k09501. The *F deletion was isolated as a cn1-bw1 recombinant chromosome from the *F cross cn1 bw1 females x cn1 *F P{PZ}l(2)0365903659/P{lacW}l(2)k09501k09501 bw1 sp1; TMS, Sb1 *F P{Delta2-3}/+ males. Polytene chromosome squashes showed the breakpoints *F 45D3-4;45F2-6. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157305 *a Andrade *b R. *c K. *d Cook *t 2003.4.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Apr 02 19:30:12 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC33 *F Isolation and characterization of Df(3L)BSC33 *F Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC33 was isolated as a P transposase-induced male recombination *F event involving P{lacW}Cdc27L7123 and P{lacW}l(3)j1D5j1D5. The deletion *F was isolated as a rhove-1-e1 recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{lacW}Cdc27L7123/P{lacW}l(3)j1D5 e1 males. Polytene chromosome *F squashes showed the breakpoints 65E10-F1;65F2-6. Df(3L)BSC33 failed to *F complement qmL14.4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157306 *a Deal *b J. *c K. *d Cook *t 2003.4.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Apr 02 19:52:52 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC31 *F Isolation and characterization of Df(2L)BSC31 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC31 was isolated as a P transposase-induced male recombination *F event involving P{EP}EP2297 and P{PZ}l(2)0627006270. The deletion was *F isolated as a net1-cn1 recombinant chromosome from the cross net1 *F cn1 b1 females x net 1 P{EP}EP2297/P{lacW}l(2)0627006270 cn1; *F TMS, P{Delta2-3}/+ males. Polytene chromosome squashes showed the *F breakpoints 23E5;23F4-5. Df(2L)BSC31 failed to complement msl-2227 and *F Pdswk10101. The deficiency chromosome retains the miniwhite marker from *F P{EP}EP2297. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157307 *a Levis *b R. *t 2003.3 *T personal communication to FlyBase *u *F From crosby@morgan.harvard.edu Thu Apr 03 16:58:20 2003 *F Subject: Alleles changes based on Levis pc *F To: curators@morgan.harvard.edu *F Personal communication to FlyBase from Robert Levis *F March 2003 *F Note:In cases in which the flanks map to different nearby sites, there is *F no way to determine from invPCR data whether there are two insertions *F or an insertion associated with a deletion. I would think that it *F would be simplest to record it as two insertions, but put in a caveat *F in a comment field to the effect that this could be two insertions or *F one insertion associated with a deletion *F KG00307 *F Previously reported to be multiple insertions 14 nt apart. This is *F incorrect. Flanking sequences map to the same site. The 3' flank begins *F with a 12 nt sequence that does not align with genomic at the insertion *F site. This sequence is found in the vector. I'm not sure whether this *F is an invPCR artifact or a sloppy P element insertion (that added extra *F nucleotides from the P-element), but it does NOT appear to be a double *F insert. *F KG00799 *F 2 insertions within Dscam; flanking sequences map 169 nt apart on opposite *F strands. *F KG06827 *F 2 insertions within Hnf4; flanking sequences map 11 nt apart. *F KG00542 *F 2 insertions; flanking sequences map 2415 nt apart on opposite strands. *F The 3' flank is in the 5' UTR of PGRP-LF (CG4437) and 5' flank is in *F CDS of CG4347. *F KG01370 *F Previously reported to be multiple insertions 18 nt apart. This is *F incorrect. Flanking sequences map to the same site. *F KG03309 *F Two insertions 2.5 kb apart or one mael insertion and a 2.5 kb deletion. *F KG03939 *F 2 insertions; flanking sequences map 484 nt apart on opposite strands. *F KG01645 *F No evidence for more than one insertion. *F KG05879 *F 2 insertions; flanking sequences map 72 nt apart. *F KG01037 *F No evidence for more than one insertion. *F KG01248 *F There is marginal evidence for a 2nd insert at an undetermined site. *F The 5' flank is 139 nt long and aligns at the site recorded for *F KG01248a. The 3' flank was 29 nt long in the original and 33 nt long *F in the recheck (the 29 nt is a subset of the 33 nt). The 3' flank *F and 5' flanks do not have 8 nt target site duplication. The 3' flank *F is not only short, but has a low complexity because of a run of 13 *F T's. When I BLAST it vs all Dros sequences, the 3' flank does not *F give any really good alignments. *F KG00877 *F 2 insertions; flanking sequences map 16 kb apart, 5' CG17741, 3' *F unknown. *F KG03323 *F No evidence for more than one insertion. *F KG00779 *F No evidence for more than one insertion. *F KG00872 *F Previously reported to be multiple insertions 24 nt apart. This is *F incorrect. Flanking sequences map to the same site. *F KG02394 *F No evidence for more than one insertion. *F KG02862 *F Short 5' flank that mapped to 2L, but didn't reappear on recheck. *F Perhaps this should be entered as a single insert with a comment *F that there is a possibility of a 2nd insert at 2L-21E4-21F1. *F KG03784 *F No evidence for more than one insertion. *F KG04515 *F No evidence for more than one insertion. *F KG05115 *F No evidence for more than one insertion. *F P{GT1}cher[BG02734a] *F The flanks of BG02734 map 8 nts apart on opposite strands. Perhaps a *F tandem double insert? *F P{SUPor-P}KG01280a *F No evidence for a 2nd insertion for KG01280. # *U FBrf0157308 *a Bloomington Drosophila Stock Center *b ?. *t 2003.4.9 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Wed Apr 09 17:11:39 2003 *F Subject: symbol for protoporphyrinogen-oxidase *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: gene symbol for protoporphyrinogen-oxidase *F We need a gene symbol for protoporphyrinogen-oxidase and apparently *F none has yet been assigned. We will use Ppox. # *U FBrf0157309 *a Whitfield *b W.G.F. *t 2003.4.8 *T personal communication to FlyBase *u *F From FlyBase-error@ogre.lbl.gov Tue Apr 08 14:16:29 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: FlyBase error report for CG6384 on Tue Apr 8 07:15:52 2003 *F Error report from William G F Whitfield (w.g.f.whitfield@dundee.ac.uk) *F Gene or accession: CG6384 *F Release: 3 *F Missed gene *F Comments: This is not really to report an error, but to suggest that the gene *F name be changed from Cen190 to Cp190. Although I suggested the current name *F some considerable time ago, I have since reached agreement with other workers *F in the field to call the expressed protein CP190 (previously referred to as *F Bx63 antigen or DMAP190 \- see Oegema et al., 1995 J. Cell Biol. 131, *F 1261-1273). I am currently writing a manuscript to report the derivation and *F characterisation of fly lines that are mutant for this gene, and I have come *F to the conclusion that changing the name of the gene to correspond with its *F protein product would help to avoid confusion in future reports. *F Browser: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.0) *F Accessed from: /local/fruitfly.org_80/cgi-bin/annot/batch.pl, *F /local/fruitfly.org_80/htdocs # *U FBrf0157311 *a Johansen *b K. *t 2003.4.23 *T personal communication to FlyBase *u *F From kristen@iastate.edu Wed Apr 23 16:01:02 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10380 *F Dear Rachel, *F You previously had asked about the CG number for a gene we named *F Chromator that we reported in a Drosophila meeting abstract. *F Chromator corresponds to CG10712. *F Let me know if you need any other information. *F Thanks! *F Best regards, *F \-Kristen *F Kristen M. Johansen, Ph.D. *F Professor of Zoology and Genetics *F 3154 Molecular Biology Building *F Iowa State University *F Ames, Iowa 50011 *F phone:515-294-7959 *F fax: 515-294-4858 *F e-mail: kristen@iastate.edu *F From kristen@iastate.edu Wed Apr 23 18:50:32 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: ADRC-10380 *F >Dear Kristen, *F > *F >Many thanks for this \- will curate as a personal communication from you *F >to FlyBase and make Chromator be the valid symbol for CG10712. *F > *F >Best regards, *F > *F >Rachel. *F Hi Rachel- *F Sounds good, and thanks for updating this. For a short symbol for *F Chromator could we use Chro? I don't think it has been taken yet but *F if so, let me know, and I'll look for another. *F Thanks! *F \-Kristen *F Kristen M. Johansen, Ph.D. *F Professor of Zoology and Genetics *F 3154 Molecular Biology Building *F Iowa State University *F Ames, Iowa 50011 *F phone:515-294-7959 *F fax: 515-294-4858 *F e-mail: kristen@iastate.edu # *U FBrf0157313 *a Fischer *b J.A. *t 2003.4.30 *T personal communication to FlyBase *u *F From jaf@mail.utexas.edu Wed Apr 30 17:07:17 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: something's wrong with nudC *F Something's wrong with the links for the gene nudC. The gene isn't *F indicated on the map, and the links to the sequence aren't there. *F \-- *F Janice A Fischer, PhD *F Associate Professor *F The University of Texas at Austin *F Institute for Cellular and Molecular Biology *F 2500 Speedway-MBB *F Austin, TX 78712 *F office phone: 512-471-5831 *F lab phone: 512-471-2826 *F fax: 512-232-3432 *F e-mail: jaf@mail.utexas.edu *F http://www.icmb.utexas.edu *F >From rd120@gen.cam.ac.uk Wed Apr 30 17:24:59 2003 *F To: flybase-help@morgan.harvard.edu, jaf@mail.utexas.edu *F Subject: Re: something's wrong with nudC *F Hi Janice, *F We don't seem to know anything molecular about this gene, which is why *F it is not mapped to the sequence. I've just now checked the paper *F FBrf0098222 == Cunniff et al., 1997, Mech. Dev. 66(1-2): 55--68 *F and see it includes sequence (Figure 1) which was not (apparently) submitted *F to Genbank. This means that we had nothing to go on in drawing the *F correspondence between the gene record and any CG gene annotation. *F I'll check it out by BLAST and get back to you. *F Best regards, *F Rachel. *F for FlyBase curators *F >From jaf@mail.utexas.edu Wed Apr 30 18:08:06 2003 *F Subject: Re: something's wrong with nudC *F To: Rachel Drysdale (Genetics) *F It looks like it's CG9710 (I BLASTed it) *F >From rd120@gen.cam.ac.uk Thu May 01 11:07:13 2003 *F To: jaf@mail.utexas.edu *F Subject: Re: something's wrong with nudC *F >It looks like it's CG9710 (I BLASTed it) *F yup \- definitely best hit by far for DNA and protein, and map location *F concurs. I'll curate our correspondence as a personal communication *F from you to FlyBase and that will merge the gene records for nudC and *F CG9710 (nudC will become valid symbol, CG9710 the synonym). Great. *F Regards, *F Rachel. # *U FBrf0157314 *a Levis *b R. *t 2003.4.25 *T personal communication to FlyBase *u *F From levis@ciwemb.edu Fri Apr 25 16:39:46 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: l(3)s3635 is inserted in CG8947 *F I have unpublished information on the mutant l(3)s3635 that you may *F want to curate as a personal communication. The flanking sequence *F (GenBank AQ026395 ) of the P-element insertion in l(3)s3635, aligns *F with scaffold segment AE003536 with a small indel. The insertion *F site maps to coordinate 254413, which is at \+8 of the 5' UTR of the *F 26-29 kD proteinase gene (CG8947), according to the current release 3 *F annotations. Another P-element, KG00154, is inserted at the same *F nucleotide site; KG00154 is homozygous viable and fertile, raising *F the possibility that the P-element insertion in l(3)s3635 may not be *F responsible for the lethality. Both l(3)s3635 and KG00154 are *F currently available from the Bloomington Stock Center. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0157315 *a Rong *b Y. *t 2003.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 28 18:14:54 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Rong insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Yikang Rong, National Cancer Institute (4/03). *F P{y-donor}1B is a third chromosome insertion. *F P{w8,I-site}7 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157316 *a Bellen *b H. *t 2003.4.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 28 20:00:06 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Mapping of P{lacW}M84 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Hugo Bellen, Baylor College of Medicine. *F P{lacW}M84 is a homozygous viable and fertile insertion at 44C. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157317 *a Reuter *b G. *t 2003.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 28 20:54:18 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Reuter P{RS5} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Gunter Reuter, University of Halle (4/03). *F The following are insertions on SM6a. Insertion sites and transposition *F frequencies are shown. *F P{RS5}5-HA-1005 unknown on 2 22.7% *F P{RS5}5-HA-1006 46B13-E4 18.6% *F P{RS5}5-HA-1007 52F7-53C 23.9% *F P{RS5}5-HA-1026 52F7-53C2 28.5% *F P{RS5}5-HA-2484 is an insertion on Dp(1;Y)y+ with a transposition *F frequency of 40.8%. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157318 *a Rawls *b A. *c T. *d Wolff *t 2003.4.28 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Apr 28 21:54:39 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: stan and pk mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Amy Rawls and Tanya Wolff, Washington University in St. *F Louis (3/03). *F stan192 is an X ray-induced null allele showing pattern polarity *F phenotypes in the ommatidia, wing, thorax and leg. *F stanfrz3 is a hypomorphic allele discovered by David Gubb showing *F polarity phenotypes in the ommatidia, wing, thorax and leg. *F pkeq is a null allele discovered by Tian Xu showing polarity phenotypes *F in the ommatidia, wing, thorax and leg. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157319 *a Deal *b J. *c K. *d Cook *t 2003.4.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Apr 29 16:03:51 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC30 *F Isolation and characterization of Df(2L)BSC30 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC30 was isolated as a P transposase-induced male recombination *F event involving P{PZ}l(2)0143301433 and P{lacW}l(2)k00302k00302. The *F deletion was isolated as a dp+-cn+ recombinant chromosome from the *F cross dpov1 cn1 bw1 females x P{PZ}l(2)0143301433 cn1/dpov1 *F P{lacW}l(2)k00302k00302; TMS, P{Delta2-3}99B males. The miniwhite marker *F from P{lacW}l(2)k00302k00302 was disrupted or deleted based on eye color *F upon outcrossing to w- flies. Polytene chromosome squashes showed the *F breakpoints 34A3;34B7-9. Df(2L)BSC30 failed to complement Nnp-1k07826 and *F l(2)k05911k05911, but complemented the visible phenotype of nub2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0157320 *a Rao *b S. *c D. *d Deitcher *t 2003.4.8 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Thu May 01 19:22:37 2003 *F Subject: P{UAS-preproANF-EMD}136.3 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Sujata Rao and David Deitcher, *F Cornell University *F To: Bloomington Drosophila Stock Center *F Subject: P{UAS-preproANF-EMD}136.3 *F Dated: 8 April 2003 *F Information communicated: The insertion identifier of the X *F chromosome insertion of P{UAS-preproANF-EMD} described in *F Rao et al., J. Neurobiol. 49:159, 2001 (FBrf0141613) is 136.3. # *U FBrf0157321 *a Morin *b X. *c W. *d Chia *t 2002.12.21 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Fri Apr 25 21:51:43 2003 *F Subject: Sequence of P{PPT-GA}, P{PPT-GB}, P{PPT-GC} *F To: flybase-updates@morgan.harvard.edu *F *F Personal communication from: Xavier Morin, Developmental Biology *F Institute of Marseilles, and William Chia, King's College London *F To: Bloomington Drosophila Stock Center *F Subject: Sequence of P{PPT-GA}, P{PPT-GB}, P{PPT-GC} *F Dated: 21 Dec 2002 *F *F Background: Xavier has provided an html file with relevant links *F and color-coding of various annotations of the sequence of these *F three constructs. Stocks carrying these constructs are now in the *F stock center, but the sequence information is not available. *F *F File deposited: Morin.2002.12.21.html; File date: 2002.12.21; File *F size: 16389; File format: html # *U FBrf0157338 *a Richardt *b A. *c B. *d Hovemann *t 2003.1.30 *T personal communication to FlyBase *u FlyBase error report for CG32099 on Thu Jan 30 03:02:15 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 30 Jan 2003 03:02:15 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: Bernhard.Hovemann@ruhr-uni-bochum.de *F Subject: FlyBase error report for CG32099 on Thu Jan 30 03:02:15 2003 *F Error report from Arnd Richardt, Bernhard Hovemann* *F (Bernhard.Hovemann@ruhr-uni-bochum.de) *F Gene or accession: CG32099 *F Release: 3 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG32099. *F Comments: \*correspondence to *F Our in vitro assays showed that the coded protein transfers phosphopantetheine *F to the thiolation domain of the ebony protein (FBgn 0000527). This reaction *F depends on CoA and is therefore homologous to the reaction catalyzed by *F microbial phosphopantetheine transferases. We therefore propose that CG32099 *F is a Drosophila phosphopantetheine transferase and since it converts apo-Ebony *F to holo-Ebony named it Ebony Activating Protein (EAP). # *U FBrf0159398 *a FlyBase Curators *b ?. *t 2002- *T personal communication to FlyBase *u Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. *F The Gene Ontology (GO) Consortium created the evidence code 'ND' *F to indicate 'No biological data available'. This code is used for *F annotations to the three terms 'molecular_function unknown', *F 'biological_process unknown' or 'cellular_component unknown'. *F *F In FlyBase the use of any of these three GO terms, attributed to this *F reference and supported by the ND evidence code, signifies that a *F curator has examined the available literature and sequence for this *F gene and that as of the date of the annotation to the unknown term, *F there is no information supporting an annotation to any GO term in *F that ontology. # *U FBrf0159399 *a Shearn *b A. *t 1995.5.3 *T personal communication to FlyBase *u *F From bio_cals@jhu.edu Wed May 3 17:15:02 1995 *F Date: Wed, 03 May 1995 12:09:47 -0400 (EDT) *F Date-Warning: Date header was inserted by jhmail.hcf.jhu.edu *F From: bio_cals@jhu.edu (Allen Shearn) *F To: flybase-updates@morgan.harvard.edu *F Mime-Version: 1.0 *F Content-Type: text/plain; charset='us-ascii' *F Content-Transfer-Encoding: 7BIT *F Content-Length: 1324 *F Status: RO *F X-Lines: 40 *F *F In the Redbook there are classes of l(3)discs small and l(3) discless *F mutations. These are synonymous with l(3)SG mutations. *F *F ds1=SG56 ds2=SG26 ds3=SG66 ds4=SG42 ds5=SG29 *F ds6=SG37 ds8=SG49 ds9=SG41 ds10=SG20 *F ds11=SG8 ds12=SG17 ds13=SG60 ds14=SG31 ds15=SG67 *F *F ds16=SG34,which is allelic to l(3)ry100 of Berg and Spradling (1992) and *F has been renamed all discs small (ads) *F ds17=SG27 *F *F dsl1=SG13 dsl2=SG68 dsl3=SG25 (3-41.4) dsl4=SG1 *F dsl5=SG44 dsl6=SG40 dsl7=SG58 dsl8=SG61 dsl9=SG62 *F dsl10=SG43 *F *F l(3)SG29 has been renamed minidiscs (mnd) by Allen Shearn *F *F l(3)SG67 has been renamed diminished discs (dim) by Allen Shearn *F *F l(3)SG7 has been renamed many discs missing (mdm) by Allen Shearn *F *F l(3)SG8 has been renamed microdiscs (mcd) by Allen Shearn *F *F l(3)SG56 has been renamed quartet (qrt) by Clarissa Cheney *F *F l(3)SG33 which is = l(3)85Ea has been renamed hyperplastic discs (hyd) by *F Allen Shearn ( in Mansfield et al. 1994). *F *F The cytogenestic location of ash1 has ben refined to 76B6-11 (Tripoulas et *F al. 1994). *F *F Sincerely, *F *F Allen Shearn *F *F ALLEN SHEARN *F Department of Biology Johns Hopkins University Baltimore, MD 21218 *F phone 410-516-7285 FAX 410-516-5213 # *U FBrf0159686 *a Aigaki *b T. *t 2003.4.9 *T personal communication to FlyBase *u *F Date: Wed, 9 Apr 2003 10:02:01 \+0900 *F To: Chihiro Yamada *F From: stephen cohen *F Subject: Re: FlyBase Query (cy1880) *F Hi Chihiro, *F .. *F We used the tubulin alpha 1 promoter from the Tubulin>CD2>hh *F construct (Basler & Struhl 1994). I don't know from which tubulin *F locus it was derived. This was cloned as an EcoRI-KpnI fragment into *F Casper 4, followed by EGFP (clontech EGFP-N1) as a KpnI-NotI *F fragment. This construct is described first in Brennecke et al 2003. *F Steve *F >Dear Dr Cohen, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Brennecke et al., 2003, Cell 113(1): 25--36 *F > *F >I have a quick question I was hoping you could answer for me. *F > *F >Tub-EGFP *F >======== *F >You use a Tub-EGFP as a starting point for your bantam sensor *F >construct. I'm not sure whether we have this construct in our records *F >yet. *F > *F >Where did you get it from? Has it been published before, if so what was *F >it called? Can you tell me which Tubulin promoter was used to drive *F >expression of EGFP? *F > *F >&agr;Tub67C *F >&agr;Tub84B *F >&agr;Tub84D *F >&agr;Tub85E *F > *F > *F >Best wishes, *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), http://fly.ebi.ac.uk:7081/ *F >Department of Genetics, *F >University of Cambridge, email: c.yamada@gen.cam.ac.uk *F >Downing Street, Tel : 01223-333963 *F >Cambridge, CB2 3EH, FAX : 01223-333992 *F >United Kingdom. Memes don't exist. Spread the Word. *F >---------------------------------------------------------------------- *F \-- *F Stephen Cohen *F Developmental Biology Programme *F EMBL *F Meyerhofstr 1 *F 69117 Heidelberg, Germany *F telephone \+49 6221 387 414 *F secretary \+49 6221 387 165 *F fax \+49 6221 387 166 # *U FBrf0159689 *a Tully *b T. *c J. *d Dubnau *t 2003 *T personal communication to FlyBase *u *F Date: Thu, 5 Jun 2003 16:49:36 \-0400 *F To: Chihiro Yamada *F From: tully *F Subject: Re: FlyBase Query (cy1907/8) *F Hi, *F Josh and I have discussed these issues and have the following ideas: *F > *F >Rex *F >=== *F >You name one of your mutants Rex. The insertion hasn't been localised *F >to a particular known gene, so I have made a new gene. However we *F >already have genes called 'Rex' (Ribosomal exchange) and 'rex' (rapid *F >exhaustion). What would you like me to call this gene? *F how about 'Pavlov's Rex' \-- prx *F >If could tell me what symbols you would like for each gene, *F >I'll change my curation accordingly. *F > *F >valiet val *F >tungus tun *F >barbos barb *F >laska *F >ikar *F >rogdi *F >arleekin arl *F >pastrel pst *F >toi *F >rafael rafl *F >milkah mil *F >avgust avg *F >murashka mura *F >beluy *F >mampus mmp *F >novichok nov *F >mirta *F >rijiy *F >diana *F >zolotistuy zol *F >rosa *F >gryzun gry *F >chyorny chyo *F >moladietz mol *F >dikar *F >nord *F >drujok dru *F >jack *F >martik mrt *F >premjera prem *F >visgun vsg *F >zloday zlo *F >john *F > *F >Any new information you give me will be curated as a personal *F >communication from you to FlyBase. *F > *F OK. Two of these mutants have not yet been identified molecularly. I'll *F let you know when we know (we have done so tentatively and now are *F confirming...) *F .. *F cheers, *F tim # *U FBrf0159722 *a Giniger *b E. *t 2003.7.28 *T personal communication to FlyBase *u *F Date: Mon, 28 Jul 2003 09:50:32 \-0700 *F To: Chihiro Yamada *F From: Ed Giniger *F Subject: Re: FlyBase Query (cy1992) *F >Dear Dr Giniger, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Crowner et al. 2003, Curr. Biol. 13(11): 967--972 *F > *F >I have a few questions I was hoping you could answer for me: *F > *F >UAS-Dl *F >====== *F the UAS-Dl is the one used in the Doherty paper (what the paper *F neglects to mention is that I'm the one who made it and gave it to *F them) *F >You use a UAS-Dl construct. We have 4 of these constructs in our *F >records \- is it one of these? *F > *F > *F >DlScer\UAS.cDa *F > Doherty et al., 1996, Genes Dev. 10(4): 421--434 *F > Okajima and Irvine, 2002, Cell 111(6): 893--904 *F > Panin et al., 1997, Nature 387(6636): 908--912 *F > *F > *F >DlScer\UAS.cHa *F > Jonsson and Knust, 1996, Dev. Genes Evol. 206(2): 91--101 *F > Klein et al., 1997, Dev. Biol. 189(1): 123--134 *F > Fanto and Mlodzik, 1999, Nature 397(6719): 523--526 *F > *F > *F >DlScer\UAS.cJa *F > Baonza and Freeman, 2001, Development 128(20): 3889--3898 *F > Hukriede et al., 1997, Development 124(17): 3427--3437 *F > Lear et al., 1999, Mech. Dev. 88(2): 207--219 *F > *F > *F >DlScer\UAS.cLa *F > Lee et al., 2000, Curr. Biol. 10(15): 931--934 *F > Li and Baker, 2001, Curr. Biol. 11(5): 330--338 *F > *F > *F >If you are not sure, can you give me some more information on the one *F >that you used to help me identify it? Where did you get the line from? *F >Has it been published before? If so where? *F > *F > *F >nrtM54 *F >======== *F the source of nrtM54 is discussed in the text. The mutant that has *F traditionally been called dabM54 (from Gertler and Hoffmann) is *F actually a mutation in nrt, as was shown recently by Liebl et al *F (Development, 2003; also referenced in the text) *F >We do not have a nrt allele with this name. Can you give me some more *F >information on this allele? Where did you get the line from? Has it *F >been published before? If so where and under what name? If its a new *F >allele can you give me some details? What was the mutagen used to make *F >this allele? What are the molecular details of this allele? *F > *F > *F >trio123.4 *F >=========== *F trio 123.4 is from Eric Liebl. What he told me is that it is an *F imprecise excision of the P-element in l(3)036810 *F >We do not have a trio allele with this name. Can you give me some more *F >information on this allele? Where did you get the line from? Has it *F >been published before? If so where and under what name? If its a new *F >allele can you give mesome details? What was the mutagen used to make *F >this allele? What are the molecular details of this allele? *F > *F >sim-GAL4 *F >======== *F >You use a sim-GAL4 line in your paper. We have 2 of these constructs in our *F >records \- is it one of these? *F We got sim-GAL4 from Steve Crews, so my guess would be that it is the *F P3.7, but that is just a guess. *F > *F >Scer\GAL4sim.P3.7 *F > Battye et al., 1999, Development 126(11): 2475--2481 *F > Georgiou and Tear, 2002, Development 129(12): 2947--2956 *F > Kidd et al., 1998, Neuron 20(1): 25--33 *F > Kramer et al., 2001, Science 292(5517): 737--740 *F > Lanoue and Jacobs, 1999, Dev. Genet. 25(4): 321--330 *F > Noordermeer et al., 1998, Neuron 21(5): 991--1001 *F > Sedaghat et al., 2002, Development 129(11): 2591--2606 *F > Xiao et al., 1996, Mech. Dev. 58(1-2): 65--74 *F > Scer\GAL4 gene expression is driven by 3.7kb BamHI fragment of *F > sim 5' flanking DNA (and 18 amino acids of coding sequence) *F > containing the early sim promoter and 5' flanking sequences. *F > *F > *F >Scer\GAL4sim.PS *F > Becker et al., 1997, Development 124(13): 2615--2622 *F > Bossing and Brand, 2002, Development 129(18): 4205--4218 *F > Dorfman et al., 2002, Dev. Biol. 252(1): 119--126 *F > Golembo et al., 1996, Development 122(11): 3363--3370 *F > Hummel et al., 2000, Neuron 26(2): 357--370 *F > Luer et al., 1997, Development 124(14): 2681--2690 *F > Sanchez Soriano and Russell, 1998, Development 125(20): 3989--3996 *F > Scholz et al., 1997, Mech. Dev. 62(1): 79--91 *F > Vorbruggen and Jackle, 1997, Proc. Natl. Acad. Sci. USA *F >94(16): 8606--8611 *F > 3.5kb EcoRV sim promoter fragment drives expression of *F > Scer\GAL4. *F > *F >If you are not sure, can you give me some more information on the one *F >that you used to help me identify it? Where did you get the line from? *F >Has it been published before? If so where? *F > *F >Any new information you give me will be curated as a personal *F >communication from you to FlyBase, if thats fine with you. *F Feel free to quote me as a personal communication for any of the *F information above. *F >Best wishes, *F > *F >Chihiro *F > *F >---------------------------------------------------------------------- *F >Chihiro Yamada. *F > *F >FlyBase (Cambridge), http://fbserver.gen.cam.ac.uk:7081/ *F >Department of Genetics, *F >University of Cambridge, email: c.yamadagen.cam.ac.uk *F >Downing Street, Tel : 01223-333963 *F >Cambridge, CB2 3EH, FAX : 01223-333992 *F >United Kingdom. Memes don't exist. Spread the Word. *F >---------------------------------------------------------------------- # *U FBrf0159729 *a Rodrigues *b V. *t 2003.5.23 *T personal communication to FlyBase *u *F Date: Fri, 23 May 2003 16:43:38 \+0530 (IST) *F From: Veronica Rodrigues *F To: David Sutherland *F Our Dll-Gal4 stock was made by us (Ludwin Pinto in my lab) by conversion *F of the P(981)ry+ insertion to agal4 insertion. We verified by completion *F and by checking patterns of expression that the conversion to a P(Gal4) *F was accurate and re-capitulated the dll pattern of expression. *F Here is the data on P(981): *F Stock Number: 10981 *F Old P Stock#: P981 *F Genotype: P{ry+t7.2=PZ}Dll01092 cn1/CyO; ry506 *F Chromosome(s): 2;3 *F Breakpts/Insertion: 060E01-060E02 *F Date added: 6/01/93 *F Donor: Berkeley Drosophila Genome Proj. *F Donor's source: Allan Spradling # *U FBrf0159732 *a Christiansen *b A. *t 2003.6.4 *T personal communication to FlyBase *u *F Date: Tue, 3 Jun 2003 13:43:23 \-0700 *F To: David Sutherland *F From: christn@pmgm2.Stanford.EDU *F Subject: Fwd: FlyBase Curation Query *F A. Spradling provided two lacZ enhancer trap insertions in esg, *F esg05729 and esg05730. The insertion sites for both enhancer traps *F were mapped to the same location, 410bp upstream of the esg *F transcriptional start site. We used these enhancer trap insertions *F as targets for a P-element mediated replacement with a P{GawB} line *F (Sepp and Auld, 1999) to obtain GAL4 expressing enhancer trap lines *F in esg. We obtained 4 GAL4 insertions in the esg locus but the *F original lacZ insertion remained in each case. *F Those 4 GAL4 insertions are cleverly named A, B, C and D. All four *F recreate the esg expression pattern in embryos and larvae. I've used *F B since I had some initial problems with A. *F Audrey Christiansen *F To: bbaker@pmgm2.Stanford.EDU *F >>Subject: FlyBase Curation Query *F >>From: David Sutherland *F >>Date: Tue, 3 Jun 2003 16:21:40 \+0100 *F >> *F >>Dear Bruce, *F >> *F >>Do you have information you could send me on 'esgGAL4.B, isolated *F >>by A. Christiansen' as mentioined in your paper 'Keisman et al., *F >>2001, Dev. Cell 1(2): 215--225'. The minimum I need to know to *F >>create a record for it is whether it is an enhancer trap or a *F >>promoter/enhancer fusion? Any molecular details you can provide *F >>could also be included in a curation record and an archived *F >>presonal communication. # *U FBrf0159800 *a Luschnig *b S. *t 2003.6.11 *T personal communication to FlyBase *u *F To: flybase-help@morgan.harvard.edu, luschnig@pmgm2.stanford.edu *F Subject: Re: rpl25 vs. rpl23a *F From: Michael Ashburner (Genetics) *F Date: Wed, 11 Jun 2003 09:49:30 \+0100 *F Stefan *F Yes, I think you are correct that what FB calls RpL23a and RpL25 are indeed *F the same thing. Only RpL23a has an associated gene model and sequence. *F FB will now merge these two 'genes' into one under the name RpL23a. *F Thanks for the heads up. *F Michael Ashburner *F > From luschnig@pmgm2.stanford.edu Wed Jun 11 06:52:30 2003 *F > Envelope-to: ma11@gen.cam.ac.uk *F > Delivery-date: Wed, 11 Jun 2003 06:52:30 \+0100 *F > Mime-Version: 1.0 *F > Date: Tue, 10 Jun 2003 22:58:48 \-0700 *F > To: flybase-help@morgan.harvard.edu *F > From: Stefan Luschnig *F > Subject: rpl25 vs. rpl23a *F > *F > Hi, *F > *F > I noticed a mixup in FlyBase between rpl25 (FBgn0014878) and rpl23a *F > (FBgn0026372). *F > I was searching for the drosophila homolog of yeast rpl25, and a BDGP *F > BLAST search comes up with drosophila rpl23 as the closest homolog. *F > There is no genbank entry or any other sequence file associated with *F > rpl25 in FlyBase. *F > According to FlyBase, though, drosophila rpl23a and the elusive *F > drosophila rpl25 map to slightly different intervals (62A for rpl23a *F > vs 62C for rpl25). *F > I assume there has been some annotation problem due to the confusing *F > nomenclature for ribosomal proteins, and I assume the entries for *F > rpl25 and rpl23a are supposed to refer to the same single gene. *F > *F > regards, *F > Stefan *F > *F > *F > \-- *F > ___________________________________________________________ *F > Stefan Luschnig *F > Department of Biochemistry/HHMI *F > 279 Campus Drive, Beckman Center Rm. B453 *F > Stanford University School of Medicine *F > Stanford, CA 94305 *F > USA *F > *F > lab phone (650)-723-5872 *F > Fax (650)-723-6783 *F > E-mail luschnig@cmgm.stanford.edu *F > ____________________________________________________________ # *U FBrf0159801 *a Herr *b D. *t 2003.6.4 *T personal communication to FlyBase *u *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 4 Jun 2003 13:59:51 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: dherr@sunstroke.sdsu.edu *F Subject: FlyBase error report for CG32484 on Wed Jun 4 13:59:51 2003 *F Error report from Deron Herr (dherr@sunstroke.sdsu.edu) *F Gene or accession: CG32484 *F Release: 3 *F Gene annotation error *F Gene CG32484 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >CG32484_(Sk2)_cDNA *F CTGCCACCGACGGTAACACTGCGGCTATGATTGGATGATAAGCGATCCATAAAAGCCTGGACACAAAAGACCTGAAACA *F TGCTGCTTGGCAGCCGAATCTCCAGTCGAAATGAGCGAATCTCTTGATAAGACCACCAGCCCGAGCTCGGCCAGTTCCA *F GGGCCACGCCCCCGGGAACGCAGGATGCGGATGAGGGCCACGACGTGAGCGATACCTTCTACACGAGCCAGCGCAAGAA *F GGGCAGCCACGTATTTCGGGTGCGCCTTGACGCCACAGGATTCACCCTGCAGCGGGAGTCGCCGGGCGGTAGCATTGTT *F AAGGAGCAACATGTCCGCATATCGGACATTGTGGGTGCCCGCTGCATGCGGCCCAAGAAGAGCCGGCGCCTGGCGATGT *F CGGGCGCCTGTGCGTGCAGCTCCGGTAATCCCAATTCGCCAGCCATCTCGGCGTCCGGCGATCACCATCGCCCTGCCAC *F CACACCAAGCAAATGCAGCACCAATAGTCGGGATAATATTCCTTCGGATGGCGGCGATGTCAGCGCGTTTCTCTACGTT *F TTTGCCTATGTTCTGAAGAAGAGGAGCCTGCGGTCGGAGTTGCACCGGGAGCGAACGGTGCTCACTCTGCGCTTCCGGT *F CGTTCGACACCTTCGAGGACAACATGAGGGAGGCGGATCGTTGGTACAGATCCCTTCGCTGGCAGTTGCATCGCACGCT *F GGAGGAGATCTTTGTGGCGCCGACGGTGGATGAGCGACGCCGTCGAGTGCTTGTGCTGTTGAATCCCAAATCCGGTTCC *F GGTGACGCTCGTGAGGTCTTCAACATGCACGTGACGCCGGTGCTCAACGAGGCCGAGGTGCCCTACGACCTGTATGTAA *F CCAAGCATTCCAACTTTGCCATCGAGTTCTTGAGCACCAGGTGCCTGGACGCCTGGTGCTGCGTGGTGGCTGTCGGCGG *F AGACGGTCTCTTCCACGAGATAGTCAATGGACTGCTGCAGC! *F GCCAGGACTGGGCCCACGTCCTGCCTCATCTGGCACTGGGAATCATTCCTTGCGGCTCCGGAAATGGATTGGCCCGCT *F CCATTGCCCATTGTTACAACGAACCATACTTCAGCAAGCCAGTGCTAGGAGCTGCTCTGACCGTAATCAGTGGACGCAG *F TTCACCCATGGACGTGGTCCGGGTGCAGCTGCAGAGTCGCTCCCTCTACTCCTTCCTGTCCATCGGCTGGGGTCTGATC *F TCGGACGTGGACATCGAGAGCGAGCGCATTCGCATGTTGGGCTACCAGCGCTTCACCGTGTGGACCCTCTACCGTCTGG *F TGAATCTGCGCACCTACAACGGCCGAATCAGCTATCTTCTGACGGACCATGAGGTGTCCTCAACCCATAGCGCTACCGG *F TTATGCTGCCCAGCGGAGAATGCAGAGCAGCCGTAGCTGCAACACGCACATCGACATGCTAAATGGGCCGGCGCCCATC *F TATCATTCCAGTGCCGAGTACCTGCCACAGGAGTTTGCGGACGTGATCTCCCTGGAGACGTCCATCAATCAGTCGTTCC *F GCTCGAGGTGCGACAGCTGGTTGTCGGGGGGATCGCGGCGCAGCTTTTACTATTCCATATCGGAGAGCATCTACCACAG *F TCTGGCGGATGAGAGCGAGTTCGCCGGCCTGGCGGCCGCCTCGCTGGAAAACCGGCAGCAGAACTACGGTCCGGCAAGC *F GAGCTGCCGGATCTGAACGAACCGCTGTCCGAGGATCAGGGTTGGCTGGTGGAGGAGGGCGAGTTCGTCATGATGCACG *F CCGTTTACCAGACCCATCTGGGCATCGACTGTCATTTTGCGCCCAAGGCCCAGCTGAACGACGGCACCATCTACCTGAT *F CCTCATACGCGCCGGCATCAGCCGCCCGCACCTGCTGAGCTTCCTCTACAACATGAGCTCCGGCACTCACCTGCCGGAG *F TCGCACGACGACCATGTGAAGGTGCTGCCAGTGCGAGCATT! *F CCGCCTGGAGCCCTACGACAATCACGGCATCATCACGGTCGACGGCGAGCGCGTCGAGTTCGGGCCCCTC *F CAAGCTGAGGTCCTGCCGGGCATAGCCCGCGTCATGGTGCCCAAGTAGGAGGAGCTTACTGAAGACCATCAAGCAATAG *F AAATCTCAATTTTGGAATCTCTGCATTTGTAGCTAATACTTAGGGTCCCAGGTGGCCGATATGAGAGAGTTGTGCATTC *F TACATATTTCGTGTTTTTGTGGCCTGCTTCTGCCAACCAATCATGTATTGTTAACATTTTAAACACAATAACAGCTATT *F TCCGAAATATCTAACATGTTTGTTTATAAAACGTGTGCCATATGAAGTGCACGTGAATTTATTTTTATCTCGGCTGTTC *F AAAATAGCGATGAAAGTCTTATTTATTTTGTTCTTTTTTTTTTTAAACTGTGTAACGAAATGAGATATATATTCAAAAT *F GTTTAAAGATGAATACAAATAAATCTTCATGAATTCAAAAATCTTAGAAAGTAACAGTGTAAGTAACAGAGCTAAATCA *F TTTACAATTCCATATTTTAAAGTAGGAAGTTAAGAATATAACATCTTTGAGCTTGAAATAAAAAATAAAAATGTTAACT *F AAA *F Protein sequence: *F >CG32484_(Sk2)_aa *F MSESLDKTTSPSSASSRATPPGTQDADEGHDVSDTFYTSQRKKGSHVFRVRLDATGFTLQRESPGGSIVKEQHVRISDI *F VGARCMRPKKSRRLAMSGACACSSGNPNSPAISASGDHHRPATTPSKCSTNSRDNIPSDGGDVSAFLYVFAYVLKKRSL *F RSELHRERTVLTLRFRSFDTFEDNMREADRWYRSLRWQLHRTLEEIFVAPTVDERRRRVLVLLNPKSGSGDAREVFNMH *F VTPVLNEAEVPYDLYVTKHSNFAIEFLSTRCLDAWCCVVAVGGDGLFHEIVNGLLQRQDWAHVLPHLALGIIPCGSGNG *F LARSIAHCYNEPYFSKPVLGAALTVISGRSSPMDVVRVQLQSRSLYSFLSIGWGLISDVDIESERIRMLGYQRFTVWTL *F YRLVNLRTYNGRISYLLTDHEVSSTHSATGYAAQRRMQSSRSCNTHIDMLNGPAPIYHSSAEYLPQEFADVISLETSIN *F QSFRSRCDSWLSGGSRRSFYYSISESIYHSLADESEFAGLAAASLENRQQNYGPASELPDLNEPLSEDQGWLVEEGEFV *F MMHAVYQTHLGIDCHFAPKAQLNDGTIYLILIRAGISRPHLLSFLYNMSSGTHLPESHDDHVKVLPVRAFRLEPYDNHG *F IITVDGERVEFGPLQAEVLPGIARVMVPK *F Comments: The predicted splice site excludes 15 bp that appears in the cDNA *F sequence (LD11247). (This sequence was confirmed by our lab.) Consequently, *F there is a 5 aa deletion in the predicted protein. # *U FBrf0159820 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for CG33083 on Tue Jun 24 18:44:41 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:44:41 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG33083 on Tue Jun 24 18:44:41 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG33083 *F Release: 3 *F Gene annotation error *F Gene CG33083 has incorrect exon/intron structure or translation start site. *F Comments: I don't understand the evidence for the alternative isoform of this *F annotations, PB, which starts ±300bp into the first long ORF/exon of the PA *F annotation, which aligns nicely with the rest of the family. Unless there is *F strong evidence that the PB form is real I think it had best be removed. # *U FBrf0159821 *a Robertson *b H. *t 2003.6.16 *T personal communication to FlyBase *u FlyBase error report for CG33157 on Mon Jun 16 23:46:25 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 16 Jun 2003 23:46:25 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG33157 on Mon Jun 16 23:46:25 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG33157 *F Release: 3 *F Gene annotation error *F Gene CG33157 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >DmGr64e.cDNA *F ATGGCCAGGACCACTGGGGATCCTGCCAAGCGACGCAGGTGCATGTCTCGCATCAAGTTCTGGCGAAGATCGCGCGTGGG *F CAGCGATGCCACCTTGGGAATCATTAAATACAGAGTCGTTGAAAAAGATACAAAGCGATTTAAGCTTTCCTTGATAAAAG *F CTTGGCTTCTTCGCATCAGACAGGAGGATTATAAGTACAGTGGATCTTTCCAGGAAGCCATCAAGCCTGTCCTCATAATC *F GCGCAGATCTTTGCTTTGATGCCAGTTCGAAAAGTGAGCTCCAAGTTCGCAGAAGACCTAACCTTCACCTGGTTCAGCGT *F TCGATCCTATTACGCCCTGGTAACCATCCTGTTTTTTGGTGTTAGCTCTGGATATATGGTGGCCTTTGTGACAAGTGTGT *F CCTTTAATTTTGATTCGGTGGAGACCCTGGTCTTCTACTTGTCCATATTCCTTATCTCTTTGTCGTTTTTTCAACTGGCC *F AGAAAGTGGCCAGAAATAGCTCAGTCATGGCAGTTGGTGGAGGCCAAGTTGCCGCCTCTGAAGTTGCCCAAAGAGCGCAG *F ATCCCTGGCGCAGCACATTAACATGATCACCATCGTGGCCACCACTTGCTCGCTGGTGGAGCACATTATGAGCATGTTGT *F CCATGGGCTACTATGTCAACTCCTGTCCCCGATGGCCAGATCGTCCCATAGACAGTTTCCTGTACTTGAGCTTCTCCTCG *F GTCTTCTACTTCGTGGACTACACACGCTTCCTGGGCATTGTGGGCAAGGTGGTCAATGTGCTGAGCACCTTCGCCTGGAA *F CTTTAACGACATCTTTGTGATGGCAGTTAGCGTAGCTTTGGCTGCTCGCTTTCGTCAGCTTAATGACTACATGATGAGGG *F AGGCTAGATTGCCCACCACTGTGGACTATTGGATGCAGTGCAGGATTAACTTTCGCAACCTTTGCAAGCTGTGCGAAGAG *F GTGGACGATGCCATATCCACCATTACATTGCTCTGCTTCAGCAACAATCTGTACTTCATCTGCGGAAAGATCCTAAAGAG *F TATGCAGGCCAAGCCGTCTATATGGCATGCCCTGTACTTCTGGTTCTCGCTGGTCTACCTACTTGGTCGCACCCTCATCC *F TGTCCCTGTACAGCTCCAGTATAAATGATGAGTCCAAGCGACCACTGGTAATCTTTCGATTGGTTCCCCGGGAATATTGG *F TGCGATGAGCTAAAACGCTTTTCGGAGGAGGTCCAAATGGACAATGTGGCTCTGACTGGCATGAAATTCTTTCGGCTAAC *F ACGCGGCGTTGTCATTTCGGTGGCCGGCACAATTGTGACCTACGAACTCATTTTGCTGCAATTCAATGGCGAGGAAAAGG *F TGCCCGGCTGTTTCGAAAACTGA *F Protein sequence: *F >DmGr64e *F MARTTGDPAKRRRCMSRIKFWRRSRVGSEATLGIIKYRVVEKDTKRFKLSLIKAWLLRIRQEDYKYSGSFQEAIKPVLII *F AQIFALMPVRKVSSKFAEDLTFTWFSVRSYYALVTILFFGVSSGYMVAFVTSVSFNFDSVETLVFYLSIFLISLSFFQLA *F RKWPEIAQSWQLVEAKLPPLKLPKERRSLAQHINMITIVATTCSLVEHIMSMLSMGYYVNSCPRWPDRPIDSFLYLSFSS *F VFYFVDYTRFLGIVGKVVNVLSTFAWNFNDIFVMAVSVALAARFRQLNDYMMREARLPTTVDYWMQCRINFRNLCKLCEE *F VDDAISTITLLCFSNNLYFICGKILKSMQAKPSIWHALYFWFSLVYLLGRTLILSLYSSSINDESKRPLVIFRLVPREYW *F CDELKRFSEEVQMDNVALTGMKFFRLTRGVVISVAGTIVTYELILLQFNGEEKVPGCFEN *F Comments: I have compared DmGr64e with the D. pseudoobscura ortholog, and this *F comparison clearly shows that the intron splice acceptor for the first intron *F is earlier by 27bp than the current annotation. Also, the current CG33157-PA *F is actually DmGr64d. # *U FBrf0159822 *a Robertson *b H. *t 2003.6.17 *T personal communication to FlyBase *u FlyBase error report for Gr64f on Mon Jun 16 23:52:20 2003. *F From flybase-error@ogre.lbl.gov Tue Jun 17 07:53:21 2003 *F Envelope-to: gm119@gen.cam.ac.uk *F Delivery-date: Tue, 17 Jun 2003 07:53:21 \+0100 *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 16 Jun 2003 23:52:21 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for Gr64f on Mon Jun 16 23:52:20 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: Gr64f *F Release: 3 *F Gene annotation error *F Gene Gr64f has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >DmGr64f.cDNA *F ATGAAGATTCTTCCGAAGCTGGAGCGCAAGTTGAGGCGGCTGAAGAAAAGGGTAACCCGGACATCACTGTTCAGAAAATT *F GGATTTGGTTCACGAGAGTGCCCGAAAAAAGGCATTTCAAGAGAGTTGTGAGACCTACAAAAATCAGATTGAGAATGAGT *F ACGAAATCAGGAATAGTTTGCCCAAGCTGTCTCGTAGCGATAAGGAGGCATTCCTCAGCGATGGAAGCTTCCACCAGGCG *F GTGGGCAGAGTGCTCCTAGTGGCGGAGTTCTTTGCCATGATGCCGGTCAAGGGTGTGACGGGCAAACATCCTTCGGATCT *F GAGCTTCTCTTGGCGCAACATACGCACCTGTTTCAGTCTGCTTTTCATCGCCTCGAGCTTAGCTAACTTTGGACTGTCGT *F TGTTTAAGGTGTTAAACAATCCGATTAGTTTCAACAGTATTAAGCCCATTATATTCCGGGGTTCTGTCCTGCTGGTCTTG *F ATAGTGGCTCTTAATCTGGCTAGGCAGTGGCCCCAGTTGATGATGTACTGGCACACGGTGGAGAAGGATCTGCCGCAGTA *F CAAGACACAGTTGACCAAGTGGAAGATGGGTCATACCATTTCCATGGTCATGCTGCTGGGAATGATGCTGTCCTTTGCGG *F AGCATATCCTGAGCATGGTATCGGCCATAAACTACGCCTCATTCTGCAACCGCACCGCCGATCCCATCCAGAACTACTTT *F CTGCGCACCAACGATGAGATCTTCTTCGTGACCAGCTACTCCACAACTCTGGCCTTGTGGGGCAAATTCCAGAATGTGTT *F TTCGACCTTCATATGGAACTACATGGACCTGTTCGTGATGATTGTGAGCATTGGACTGGCTTCGAAGTTTCGCCAGCTCA *F ACGATGATTTGCGAAACTTTAAAGGAATGAATATGGCTCCATCTTACTGGTCAGAGCGTCGTATTCAGTACAGAAATATA *F TGCATACTGTGCGACAAAATGGACGATGCCATATCACTCATAACAATGGTGTCCTTCTCCAACAACTTGTATTTCATTTG *F CGTCCAGTTGCTGAGAAGCTTGAACACTATGCCATCGGTGGCCCATGCTGTGTACTTTTACTTCTCGCTCATCTTTCTAA *F TTGGTCGCACTCTGGCGGTTTCACTGTACTCCTCGAGTGTTCATGATGAATCCAGGCTGACTTTGAGATATCTGCGCTGT *F GTGCCCAAGGAGTCCTGGTGTCCGGAGGTGAAACGATTTACGGAGGAGGTAATCAGCGATGAGGTGGCCCTCACTGGCAT *F GAAGTTTTTCCACCTCACAAGGAAGCTGGTGCTGAGTGTGGCTGGAACCATCGTCACGTACGAACTTGTTCTCATCCAAT *F TTCATGAGGACAACGACCTGTGGGACTGCGATCAGAGCTATTACTCATAG *F Protein sequence: *F >DmGr64f *F MKILPKLERKLRRLKKRVTRTSLFRKLDLVHERARKKAFQESCETYKNQIENEYEIRNSLPKLSRSDKEAFLSDGSFHQ *F AVGRVLLVAEFFAMMPVKGVTGKHPSDLSFSWRNIRTCFSLLFIASSLANFGLSLFKVLNNPISFNSIKPIIFRGSVLL *F VLIVALNLARQWPQLMMYWHTVEKDLPQYKTQLTKWKMGHTISMVMLLGMMLSFAEHILSMVSAINYASFCNRTADPIQ *F NYFLRTNDEIFFVTSYSTTLALWGKFQNVFSTFIWNYMDLFVMIVSIGLASKFRQLNDDLRNFKGMNMAPSYWSERRIQ *F YRNICILCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSVAHAVYFYFSLIFLIGRTLAVSLYSSSVHDESRLTL *F RYLRCVPKESWCPEVKRFTEEVISDEVALTGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCDQSYYS *F Comments: In the original annotation revision I sent a long time ago I made a *F mistake, revealed by comparison with the D. pseudoobscura ortholog. I missed *F an exon in the first current intron. This corrected gene structure also makes *F it more similar to its paralog, Gr64e. # *U FBrf0159823 *a Robertson *b H. *t 2003.6.17 *T personal communication to FlyBase *u FlyBase error report for CG14916 on Tue Jun 17 20:49:16 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 17 Jun 2003 20:49:16 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG14916 on Tue Jun 17 20:49:16 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG14916 *F Release: 3 *F Gene annotation error *F Gene CG14916 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >DmGr32a.cDNA *F ATGTCCCCGAACACTTGGGTAATTGAAATGCCCACGCAGAAAACGCGATCACATCCGTATCCGCGAAGAATCTCGCCGTA *F CCGGCCACCCGTCTTGAACCGCGATGCCTTCAGTAGGGATGCACCACCTATGCCAGCGAGGAACCATGATCATCCCGTTT *F TCGAGGACATTCGCACCATTTTGTCCGTGCTCAAGGCCAGTGGCTTGATGCCAATATACGAACAGGTCTCCGACTATGAG *F GTGGGTCCTCCGACCAAAACGAATGAGTTCTACTCGTTCTTCGTAAGAGGCGTGGTCCACGCCCTGACCATCTTCAATGT *F CTACAGCTTATTTACACCGATATCGGCGCAATTATTTTTCTCCTACCGCGAGACGGACAATGTGAACCAGTGGATCGAGC *F TGCTGCTCTGCATCCTAACCTATACCCTTACGGTTTTCGTTTGTGCCCACAATACCACGAGCATGTTGCGGATAATGAAT *F GAAATCCTTCAACTCGACGAGGAAGTGCGTCGCCAGTTTGGTGCCAATTTGAGCCAAAATTTTGGGTTCTTGGTAAAGTT *F TTTAGTAGGAATCACCGCTTGCCAGGCTTATATAATCGTGCTCAAAATATATGCCGTGCAAGGCGAGATCACACCCACCT *F CCTATATACTATTGGCCTTCTATGGCATCCAGAACGGTCTGACCGCCACATATATAGTATTCGCATCGGCTTTGCTCAGG *F ATTGTGTACATCCGGTTTCATTTCATCAACCAGCTGCTAAATGGTTACACCTATGGGCAGCAGCATAGGCGCAAAGAAGG *F TGGAGCACGAGCGAGGCGGCAGCGTGGTGACGTCAATCCCAATGTCAATCCTGCTCTAATGGAACATTTCCCGGAAGACT *F CGCTATTCATATACCGCATGCACAACAAACTGCTGCGTATCTACAAGGGCATCAACGATTGCTGCAACTTGATTCTGGTC *F TCGTTTCTGGGCTACTCCTTTTACACGGTCACCACCAACTGCTACAACCTCTTTGTCCAGATTACCGGCAAGGGCATGGT *F TTCACCAAACATATTGCAGTGGTGCTTCGCCTGGTTATGTCTCCACGTTTCCCTGCTGGCTTTGCTGTCACGCAGCTGTG *F GTCTGACCACCACGGAGGCCAATGCCACATCCCAAATTCTTGCAAGGGTGTATGCCAAGTCGAAGGAGTATCAGAATATC *F ATTGATAAGTTCCTTACGAAGAGCATTAAACAGGAGGTGCAATTCACGGCATATGGATTTTTTGCGATAGATAACTCCAC *F ACTATTCAAGATATTTTCGGCCGTCACAACATACTTGGTAATCTTGATTCAGTTCAAACAGCTCGAAGACTCGAAAGTAG *F AGGATCCTGTACCAGAACAAACTTAA *F Protein sequence: *F >DmGr32a *F MSPNTWVIEMPTQKTRSHPYPRRISPYRPPVLNRDAFSRDAPPMPARNHDHPVFEDIRTILSVLKASGLMPIYEQVSDY *F EVGPPTKTNEFYSFFVRGVVHALTIFNVYSLFTPISAQLFFSYRETDNVNQWIELLLCILTYTLTVFVCAHNTTSMLRI *F MNEILQLDEEVRRQFGANLSQNFGFLVKFLVGITACQAYIIVLKIYAVQGEITPTSYILLAFYGIQNGLTATYIVFASA *F LLRIVYIRFHFINQLLNGYTYGQQHRRKEGGARARRQRGDVNPNVNPALMEHFPEDSLFIYRMHNKLLRIYKGINDCCN *F LILVSFLGYSFYTVTTNCYNLFVQITGKGMVSPNILQWCFAWLCLHVSLLALLSRSCGLTTTEANATSQILARVYAKSK *F EYQNIIDKFLTKSIKQEVQFTAYGFFAIDNSTLFKIFSAVTTYLVILIQFKQLEDSKVEDPVPEQT *F Comments: Comparison with D. pseudoobscura shows that there are another 43aa *F on the N-terminus of this Gr annotation. # *U FBrf0159824 *a Robertson *b H. *t 2003.6.18 *T personal communication to FlyBase *u FlyBase error report for CG17213 on Tue Jun 17 20:51:57 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 17 Jun 2003 20:51:57 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG17213 on Tue Jun 17 20:51:57 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG17213 *F Release: 3 *F Gene annotation error *F Gene CG17213 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >DmGr33a.cDNA *F ATGATTCAAATTATGAATTGGTTTTCAATGGTCATAGGCCTCATTCCACTGAATCGCCAGCAATCGGAAACTAACTTCAT *F ACTCGACTACGCCATGATGTGCATTGTGCCCATCTTCTATGTGGCTTGCTATCTTCTCATAAATCTTAGCCACATTATTG *F GCCTCTGTTTACTGGACTCTTGCAATAGTGTTTGCAAGCTGAGCAGCCATCTCTTTATGCATTTGGGCGCATTTCTATAT *F CTGACCATCACCCTGCTGTCACTTTATCGCCGAAAGGAGTTTTTCCAGCAGTTTGATGCGAGACTTAATGACATTGATGC *F AGTTATCCAGAAATGCCAGCGGGTGGCGGAAATGGACAAGGTGAAGGTTACTGCGGTGAAACACAGTGTGGCCTATCACT *F TCACCTGGCTTTTCCTATTCTGCGTTTTCACCTTTGCCCTTTACTATGACGTCAGATCTTTGTACTTGACCTTCGGCAAT *F CTCGCCTTCATTCCGTTCATGGTGTCCAGTTTCCCATATTTGGCCGGCAGCATCATTCAGGGTGAGTTCATCTATCACGT *F GTCGGTCATCTCGCAGCGCTTCGAGCAGATTAACATGCTGCTGGAGAAGATTAACCAGGAGGCGCGCCATCGCCACGCAC *F CCCTTACCGTGTTCGATATCGAGAGCGAGGGCAAAAAGGAGCGGAAGACCGTTACACCGATTACGGTCATGGACGGCAGG *F ACGACGACAGGATTTGGCAATGAGAACAAGTTCGCCGGCGAAATGAAGCGCCAGGAGGGGCAACAAAAGAACGACGATGA *F CGATTTGGACACCAGCAACGACGAGGACGAGGATGACTTTGATTATGACAATGCCACCATCGCGGAAAATACTGGAAACA *F CATCGGAAGCCAATTTACCAGATCTCTTCAAGCTGCATGATAAAATCCTGGCGCTCAGCGTGATTACAAACGGCGAGTTT *F GGACCACAGTGTGTACCCTATATGGCGGCCTGCTTTGTGGTGAGCATCTTTGGCATTTTCCTGGAGACCAAGGTCAACTT *F CATTGTGGGCGGCAAGAGTCGACTGCTGGACTATATGACCTATTTGTACGTGATTTGGAGCTTCACCACCATGATGGTGG *F CCTACATAGTGCTTCGACTATGCTGCAATGCCAACAATCATTCCAAACAATCGGCGATGATTGTCCATGAGATTATGCAA *F AAGAAACCGGCATTTATGTTGAGCAACGATCTGTTCTACAACAAAATGAAGTCATTCACACTGCAGTTTCTTCACTGGGA *F GGGTTTCTTTCAATTCAACGGCGTGGGATTGTTTGCTCTGGACTACACATTCATTTTCTCGACTGTAAGTGCAGCCACAT *F CCTATTTAATTGTCCTGCTGCAGTTTGACATGACTGCAATTTTGCGCAACGAGGGGCTAATGTCATAA *F Protein sequence: *F >DmGr33a *F MIQIMNWFSMVIGLIPLNRQQSETNFILDYAMMCIVPIFYVACYLLINLSHIIGLCLLDSCNSVCKLSSHLFMHLGAFL *F YLTITLLSLYRRKEFFQQFDARLNDIDAVIQKCQRVAEMDKVKVTAVKHSVAYHFTWLFLFCVFTFALYYDVRSLYLTF *F GNLAFIPFMVSSFPYLAGSIIQGEFIYHVSVISQRFEQINMLLEKINQEARHRHAPLTVFDIESEGKKERKTVTPITVM *F DGRTTTGFGNENKFAGEMKRQEGQQKNDDDDLDTSNDEDEDDFDYDNATIAENTGNTSEANLPDLFKLHDKILALSVIT *F NGEFGPQCVPYMAACFVVSIFGIFLETKVNFIVGGKSRLLDYMTYLYVIWSFTTMMVAYIVLRLCCNANNHSKQSAMIV *F HEIMQKKPAFMLSNDLFYNKMKSFTLQFLHWEGFFQFNGVGLFALDYTFIFSTVSAATSYLIVLLQFDMTAILRNEGLMS *F Comments: Comparison with D. pseudoobscura shows that the donor splice site *F for the first intron should be a little earlier than in the current annotation. # *U FBrf0159825 *a Robertson *b H. *t 2003.6.18 *T personal communication to FlyBase *u FlyBase error report for CG1712 on Wed Jun 18 21:11:22 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 18 Jun 2003 21:11:22 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG1712 on Wed Jun 18 21:11:22 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG1712 *F Release: 3 *F Gene annotation error *F Gene CG1712 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >DmGr43a.cDNa *F ATGGAGATATCCCAGCCCAGCATCGGCATCTTCTACATCAGCAAAGTGCTGGCCCTGGCTCCATATGCTACGGTCCGGA *F ACTCCAAGGGCCGGGTGGAAATTGGTCGCAGCTGGCTCTTCACTGTATACTCGGCCACATTAACGGTGGTTATGGTTTT *F TCTCACTTATCGGGGTCTCCTGTTCGATGCTAACTCCGAAATTCCCGTGCGAATGAAGTCGGCTACGTCGAAGGTGGTG *F ACGGCCCTGGACGTGTCTGTGGTGGTCATGGCCATTGTATCCGGTGTTTACTGTGGCCTTTTCAGCCTGAACGACACTC *F TCGAGCTCAACGATCGACTAAACAAGATCGATAATACCCTAAATGCCTACAATAACTTTCGAAGAGATCGATGGCGAGC *F CTTGGGCATGGCTGCCGTCTCTCTGCTGGCCATTTCGATTCTGGTCGGCCTGGATGTAGGGACGTGGATGCGCATTGCC *F CAGGATATGAACATAGCTCAGTCGGATACGGAGCTCAATGTGCACTGGTACATTCCATTCTATAGTCTTTACTTCATCC *F TCACGGGCTTGCAGGTTAACATTGCCAACACGGCCTATGGACTAGGAAGGCGCTTTGGCCGTCTGAACCGAATGCTTTC *F GAGCAGCTTTCTTGCAGAGAACAATGCAACCAGTGCCATCAAGCCACAAAAGGTCAGCACCGTGAAGAACGTTAGCGTA *F AATCGGCCAGCGATGCCATCGGCACTCCACGCGAGCCTGACGAAGCTGAACGGCGAAACCTTGCCCAGTGAAGCCGCAG *F CCAAGAACAAGGGGTTGCTCCTCAAATCCCTGGCGGACAGTCACGAGTCGCTGGGAAAATGTGTCCACCTCCTGTCCAA *F CTCCTTCGGCATTGCGGTTCTCTTCATCCTGGTGTCCTGTCTGCTGCACCTCGTGGCTACGGCCTACTTCCTCTTTCTG *F GAACTGCTCAGCAAGCGGGATAACGGCTACCTGTGGGTGCA! *F GATGCTCTGGATTTGCTTTCACTTCCTGCGACTGCTCATGGTGGTGGAGCCGTGCCACTTGGCTGCCCGAGAGTCCCG *F TAAAACGATCCAGATTGTTTGCGAAATCGAGCGGAAAGTGCACGAGCCCATTCTCGCGGAGGCAGTTAAGAAGTTTTGG *F CAGCAGTTACTCGTCGTAGATGCTGACTTCTCCGCCTGCGGATTGTGCCGCGTGAACCGCACCATTCTGACATCGTTTG *F CATCCGCCATAGCCACCTATCTCGTGATTCTCATTCAGTTTCAACGGACCAATGGCTAA *F Protein sequence: *F >DmGr43a *F MEISQPSIGIFYISKVLALAPYATVRNSKGRVEIGRSWLFTVYSATLTVVMVFLTYRGLLFDANSEIPVRMKSATSKVVT *F ALDVSVVVMAIVSGVYCGLFSLNDTLELNDRLNKIDNTLNAYNNFRRDRWRALGMAAVSLLAISILVGLDVGTWMRIAQD *F MNIAQSDTELNVHWYIPFYSLYFILTGLQVNIANTAYGLGRRFGRLNRMLSSSFLAENNATSAIKPQKVSTVKNVSVNRP *F AMPSALHASLTKLNGETLPSEAAAKNKGLLLKSLADSHESLGKCVHLLSNSFGIAVLFILVSCLLHLVATAYFLFLELLS *F KRDNGYLWVQMLWICFHFLRLLMVVEPCHLAARESRKTIQIVCEIERKVHEPILAEAVKKFWQQLLVVDADFSACGLCRV *F NRTILTSFASAIATYLVILIQFQRTNG *F Comments: Comparison with D. pseudoobscura reveals an intron near the *F C-terminus that is open and in frame, but which is not open or in-frame in Dp, *F hence it should be spliced in the Dm annotation too. This removes an others *F unalignable expansion region in this protein relative to the rest of the Gr *F family. # *U FBrf0159826 *a Robertson *b H. *t 2003.6.19 *T personal communication to FlyBase *u FlyBase error report for CG30030 on Wed Jun 18 22:48:49 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 18 Jun 2003 22:48:49 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG30030 on Wed Jun 18 22:48:49 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG30030 *F Release: 3 *F Gene annotation error *F Gene CG30030 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr47b *F ATGCAACGCGACGATGGCTTTGTCTACTGTTACGGAAACTTGTACAGCCTCCTGCTCTACTGGGGATTGGTCACAATCC *F GGGTGAGAAGTCCTGATCGTGGAGGTGCGTTTTCAAACAGGTGGACTGTGTGCTATGCCCTTTTCACGCGCTCCTTTAT *F GGTTATCTGCTTTATGGCTACCGTGATGACTAAGCTGAGGGATCCCGAGATGTCCGCTGCCATGTTTGGACACCTTAGC *F CCGTTGGTCAAGGCAATATTCACCTGGGAGTGCCTCAGTTGCTCGGTCACCTACATTGAGTACTGCCTTTCGCTGGACT *F TGCAGAAGGACAGACACCTGAAACTCGTGGCTAGGATGCAAGAGTTCGATCGTTCCGTTTTGATGGTGTTCCCTCATGT *F TCAGTGGAACTATCGACGCGCTCGCTTGAAGTATTGGTATGGCACTGTGATTGTCGGCTTTTGCTTTTTCTCCTTTTCC *F ATCTCACTGATCTTCGATACGACGCGTTGCACATGCGGCATACCGTCCACCTTGCTGATGGCCTTCACCTACACCTTGC *F TGACCAGTTCCGTAGGATTGCTGGGTTTCGTGCACATAGGTATTATGGACTTTATAAGGGTTCGTCTGCGTCTGGTGCA *F GCAGCTGCTTCACCAACTTTACCAAGCGGATGACAGTTCAGAG---------------GTGCACGAAAGGATTGCTTAT *F CTTTTCGAAATGTCCAAGCGCTGTTCGTTTCTCCTGGCGGAGTTAAACGGAGTTTTTGGATTCGCCGCAGCTGCTGGTA *F TCTTTTATGATTTCACTATCATGACCTGCTTCGTGTACGTTATCTGCCAAAAGCTGCTGGAGAGAGAGCCCTGGGATCC *F CGAGTACGTGTACATGTTGCTGCACGTGGCCATACACACCTACAAAGTGGTCATTACTAGCACCTATGGGTATTTACTT *F CTGCGAGAGAAACGCAACTGTATGCATTTGTTGAGCCAATA! *F CTCGCGATACTTCTCCGGACAGGATGTGGCTCGCCGGAAAACGGAGGACTTTCAGCATTGGCGCATGCACAATCGGCA *F AGCGGCAATGGTGGGCAGCACAACCTTGCTAAGTGTTTCTACCATTTACCTGGTGTACAACGGAATGGCCAACTATGTG *F ATTATCCTGGTGCAGCTGCTATTTCAACAACAGCAAATCAAAGATCACCAGTTGACATCGGGCAAAGATGTGGACATTG *F TGGGCCCAATGGGACCCATCACTCACATGGATTGA *F Protein sequence: *F >Gr47b *F MQRDDGFVYCYGNLYSLLLYWGLVTIRVRSPDRGGAFSNRWTVCYALFTRSFMVICFMATVMTKLRDPEMSAAMFGHLSP *F LVKAIFTWECLSCSVTYIEYCLSLDLQKDRHLKLVARMQEFDRSVLMVFPHVQWNYRRARLKYWYGTVIVGFCFFSFSIS *F LIFDTTRCTCGIPSTLLMAFTYTLLTSSVGLLGFVHIGIMDFIRVRLRLVQQLLHQLYQADDSSEVHERIAYLFEMSKRC *F SFLLAELNGVFGFAAAAGIFYDFTIMTCFVYVICQKLLEREPWDPEYVYMLLHVAIHTYKVVITSTYGYLLLREKRNCMH *F LLSQYSRYFSGQDVARRKTEDFQHWRMHNRQAAMVGSTTLLSVSTIYLVYNGMANYVIILVQLLFQQQQIKDHQLTSGKD *F VDIVGPMGPITHMD *F Comments: Comparison with D. pseudoobscura shows an extended N-terminus and a *F slightly earlier donor splice site for the first exon, compared with the *F current annotation in Dm. # *U FBrf0159827 *a Robertson *b H. *t 2003.6.19 *T personal communication to FlyBase *u FlyBase error report for CG32395 on Thu Jun 19 22:01:09 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 19 Jun 2003 22:01:09 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG32395 on Thu Jun 19 22:01:09 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG32395 *F Release: 3 *F Gene annotation error *F Gene CG32395 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr65a *F ATGCGCGTACATCAGCGGCAAAGTGCGGTCATAATTCAAATGGGGCACCCTCCATTCATGTCCTTGAAGGGCGGCAAATC *F GGGTTTCGGATCAATTGTTTGGCCATCCGCGATGAGGGAAGTGAATCTGCTCAACCGCTTTACACGCCAGTTCCTGTTTC *F TCATCGTGTTGGTGACCCAGATCTGCGGGGTCGCCACCTTTGTGTACAACTCGAAGGCGCAATGCTTTCGTCAGTCCGGA *F TTTCTGCGGTTCTACTCCAGCTTAGTTCTCATTTTTCTGGCACTTTTCCTGATTGTTACCACGAGTAAAATGTTTCATAA *F TCTGCAAGCTGTGTGGCCATATGTGGTAGGAAGTGTTATCATATTGGTGGTAAGAATACACGGACTTTTGGAAAGTGCAG *F AGATCGTGGAGTTGCTAAACCAAATGCTGAGAATCATGAGGCAGGTGAATCTAATGGCCAGGCACCCGAATCTGTTTCGC *F CTGAAACATTTGCTGCTCCTTCTTTTGGCCCTGCAAAATCTTTTAAGATCACTGAATACGATAGTGGGAATAAGTAACCA *F CTCTGCTGAAGCTTATGACTCTTTTCTTAATAGCGTTATCCTATTAATTATACTGGCCGTCCTGCTGAGCTTTCTTCTTC *F AGATCACCATCAATATTTGCCTCTTTGTAGTGCTCATTGCCACGTATAGCGAACTACACCATTGCACTCGACGAATCTCA *F AATGATATGGATAAGCTCAGACTTCATTCTGTCCATGAAAGTGGTCAATTTATGGTGTTGGTAAAACAACTTCAAGGAAT *F CACTGAAAAATTAATTCGACTGCGTCAAAATGTCTTTCATATTACCGTCAGAATCATACGGCATTTCCGATTTCATTGGC *F TGTGTGCTATTATCTACGGATTATTACCATTCTTTAGTTTAACAGCTAAAGATCAAAATGGTTTTAACTTCCTCATCATT *F TCCGCATTGAACATAATATTCCAGTGGACTATTTTTGCGATTCTTTCTCGTGAATCAAGAATCACCCGGAGTTTATGCAC *F TTTTCACTTGACCAATTACCATAAGGAAACTGCTAGAACGATTGATGAATTACTACATCAGGAAATTTGGGAACGTATCA *F CAGTCACCATATACGGCAATACACTCGACACGAAATTGCTGTTCAAACTGCTATCCATTAGTGCCTTTTGCGCGTTTGTT *F AACCGACTGGAGTACTTGCACATCTAG *F Protein sequence: *F >Gr65a *F MRVHQRQSAVIIQMGHPPFMSLKGGKSGFGSIVWPSAMREVNLLNRFTRQFLFLIVLVTQICGVATFVYNSKAQCFRQSG *F FLRFYSSLVLIFLALFLIVTTSKMFHNLQAVWPYVVGSVIILVVRIHGLLESAEIVELLNQMLRIMRQVNLMARHPNLFR *F LKHLLLLLLALQNLLRSLNTIVGISNHSAEAYDSFLNSVILLIILAVLLSFLLQITINICLFVVLIATYSELHHCTRRIS *F NDMDKLRLHSVHESGQFMVLVKQLQGITEKLIRLRQNVFHITVRIIRHFRFHWLCAIIYGLLPFFSLTAKDQNGFNFLII *F SALNIIFQWTIFAILSRESRITRSLCTFHLTNYHKETARTIDELLHQEIWERITVTIYGNTLDTKLLFKLLSISAFCAFV *F NRLEYLHI *F Comments: Comparison with D. pseudobscura shows a clear C-terminal intron and *F exon for this gene. # *U FBrf0159828 *a Robertson *b H. *t 2003.6.21 *T personal communication to FlyBase *u FlyBase error report for CG14901 on Fri Jun 20 23:00:54 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 20 Jun 2003 23:00:54 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG14901 on Fri Jun 20 23:00:54 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG14901 *F Release: 3 *F Gene annotation error *F Gene CG14901 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr89a *F ATGTTGCGATTTCCGCATGTCTGCGGTCTTTGTTTGCTGCTTAAATATTGGCAGATTTTGGCCTTGGCCCCGTTTCGGAC *F TTCCGAGCCAATGGTCGCCAGGTGCCAGCGATGGATGACCTTGATTGCGGTCTTTAGATGGCTACTACTCACATCAATGG *F CGCCCTTTGTTCTCTGGAAAAGTGCTGCCATGTATGAGGCCACTAATGTGCGGCACTCGATGGTCTTTAAGACAATCGCT *F TTGGCCACCATGACGGGTGATGTCTGCATTTCACTAGCTCTGTTGGGAAACCACCTTTGGAATCGCCGGGAGTTGGCCAA *F TTTGGTAAATGACTTGGCCAGATTGCATCGTCGGAGGAGACTGAGCTGGTGGTCCACATTGTTCCTCTGGCTTAAGTTGC *F TACTCTCCCTCTACGACCTGTTATGTAGTGTGCCATTTCTCAAAGGAGCCGGCGGTCGTCTGCCGTGGTCCCAACTCGTG *F GCCTATGGAGTCCAACTCTACTTTCAGCACGTGGCCAGTGTCTATGGAAACGGAATATTCGGTGGAATTCTTTTAATGCT *F AGAGTGCTACAACCAATTGGAGCGTGAGGAACCTACCAACCTGGCTAGACTCTTGCAAAAGGAATACAGCTGGCTGAGAT *F TGATCCAAAGATTCGTAAAGCTCTTTCAGCTGGGCATCTTTCTGCTGGTCCTTGGCAGCTTCGTTAATATAATGGTCAAT *F ATATACGCATTTATGTCCTACTATGTATCGCTGCATGGAGTTCCCCTGACCATATCCAATAACTGCCTAGTCCTGGCCAT *F CCAACTATATGCAGTCATTTTGGCTGCTCATCTTTGCCAGGTGAGGTCTGCTAAATTGAGAAAAAAATGCTTGCAACTGG *F AATATGTGCCCGAAGGATTGACACAGGAGCAGGCCATGGCTTCGACTCCATTTCCTGTGCTCACGCCCACTGGCAATGTC *F AAGTTTCGTATTTTGGGCGTTTTCATTCTTGACAACTCGTTTTGGCTTTTCTTGGTCTCGTATGCGATGAACTTCATAGT *F GGTTATTCTGCAGACTAGCTTTGAGCATATAAATCATGGTGAAATCTAA *F Protein sequence: *F >Gr89a *F MLRFPHVCGLCLLLKYWQILALAPFRTSEPMVARCQRWMTLIAVFRWLLLTSMAPFVLWKSAAMYEATNVRHSMVFKTIA *F LATMTGDVCISLALLGNHLWNRRELANLVNDLARLHRRRRLSWWSTLFLWLKLLLSLYDLLCSVPFLKGAGGRLPWSQLV *F AYGVQLYFQHVASVYGNGIFGGILLMLECYNQLEREEPTNLARLLQKEYSWLRLIQRFVKLFQLGIFLLVLGSFVNIMVN *F IYAFMSYYVSLHGVPLTISNNCLVLAIQLYAVILAAHLCQVRSAKLRKKCLQLEYVPEGLTQEQAMASTPFPVLTPTGNV *F KFRILGVFILDNSFWLFLVSYAMNFIVVILQTSFEHINHGEI *F Comments: Comparison with D. pseudoobscura shows this to be a previously *F unrecognized member of the gustatory receptor or Gr family. It needs a further *F upstream start and an intron and C-terminal exon to encode the typical 7 TM *F domains of these proteins, and has a good Dp ortholog that confirms this *F structure. From its cytological position in 89D2, following the convention for *F the rest of the family, it should be named Gr89a. # *U FBrf0159829 *a Robertson *b H. *t 2003.6.21 *T personal communication to FlyBase *u FlyBase error report for CG13948 on Sat Jun 21 17:50:02 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 17:50:02 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13948 on Sat Jun 21 17:50:02 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13948 *F Release: 3 *F Gene annotation error *F Gene CG13948 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr21a *F ATGTCGTTTTGGGCAGTGAGTCGTGGCCTAACGCCGCCTTCGAAGGTAGTGCCGATGCTGAATCCCAACCAACGCCAGTT *F TCTGGAGGACGAGGTGCGCTATCGGGAGAAACTGAAGCTGATGGCTCGGGGTGATGCCATGGAGGAGGTGTACGTGCGCA *F AGCAGGAAACTGTTGACGATCCGCTGGAACTGGACAAGCACGATTCCTTCTACCAGACCACCAAGAGCCTCCTCGTGCTT *F TTCCAGATAATGGGCGTAATGCCCATTCACCGCAATCCGCCCGAGAAGAACCTGCCTAGAACGGGCTACTCCTGGGGCTC *F CAAGCAGGTCATGTGGGCCATCTTCATCTACAGCTGCCAGACGACCATTGTTGTTCTGGTGCTGCGTGAGCGCGTGAAAA *F AATTCGTCACCAGCCCGGACAAGCGCTTCGATGAGGCCATCTACAATGTCATTTTCATCAGTCTGCTCTTCACCAACTTT *F CTGCTCCCGGTGGCCAGCTGGCGGCACGGTCCCCAGGTGGCTATCTTCAAGAACATGTGGACCAACTACCAGTATAAGTT *F CTTCAAAACTACCGGCTCGCCGATCGTCTTTCCGAATCTCTACCCACTCACCTGGTCGCTGTGCGTCTTCTCCTGGCTCC *F TAAGCATCGCCATCAACCTGTCGCAGTACTTCCTGCAGCCGGACTTCCGGCTGTGGTACACATTCGCCTACTACCCCATC *F ATCGCCATGCTCAACTGCTTCTGCAGCTTGTGGTACATCAACTGCAATGCATTCGGAACTGCAAGTCGCGCTCTTTCCGA *F CGCTCTGCAGACAACCATCCGGGGCGAGAAGCCCGCCCAAAAACTCACTGAGTACCGCCATCTATGGGTGGATCTGAGCC *F ACATGATGCAGCAGCTGGGACGAGCTTACTCCAACATGTACGGCATGTACTGTCTAGTCATCTTCTTCACAACGATCATC *F GCCACTTACGGCAGCATCAGCGAAATCATCGACCACGGGGCAACCTACAAGGAGGTGGGTCTGTTCGTCATCGTGTTCTA *F CTGCATGGGTCTGCTGTACATTATCTGCAACGAGGCGCACTACGCCTCCCGAAAGGTCGGACTTGACTTCCAGACGAAGC *F TGCTCAATATCAACCTGACTGCCGTGGACGCTGCCACCCAGAAGGAAGTGGAGATGCTGCTTGTGGCCATAAACAAGAAC *F CCGCCCATCATGAATCTGGACGGGTATGCAAACATCAACCGCGAGCTGATCACGACCAACATCTCGTTCATGGCCACCTA *F CCTCGTGGTCCTGCTGCAGTTTAAGATCACGGAGCAGAGACGCATTGGTCAGCAGCAGGCCTAG *F Protein sequence: *F >Gr21aMSFWAVSRGLTPPSKVVPMLNPNQRQFLEDEVRYREKLKLMARGDAMEEVYVRKQETVDDPLELDKHDSFYQT *F TKSLLVL *F FQIMGVMPIHRNPPEKNLPRTGYSWGSKQVMWAIFIYSCQTTIVVLVLRERVKKFVTSPDKRFDEAIYNVIFISLLFTNF *F LLPVASWRHGPQVAIFKNMWTNYQYKFFKTTGSPIVFPNLYPLTWSLCVFSWLLSIAINLSQYFLQPDFRLWYTFAYYPI *F IAMLNCFCSLWYINCNAFGTASRALSDALQTTIRGEKPAQKLTEYRHLWVDLSHMMQQLGRAYSNMYGMYCLVIFFTTII *F ATYGSISEIIDHGATYKEVGLFVIVFYCMGLLYIICNEAHYASRKVGLDFQTKLLNINLTAVDAATQKEVEMLLVAINKN *F PPIMNLDGYANINRELITTNISFMATYLVVLLQFKITEQRRIGQQQA *F Comments: This is really picky, but in the interests of the best annotation I *F can offer, I suggest that the first 7 amino acids be removed from the *F N-terminus of this protein. This suggestion is based on comparisons with D. *F pseudoobscura in which this protein is extraordinarily conserved at about 95% *F aa identity. Both Dm and Dp have possible N-terminal extension up to 47 and *F 55aa respectively, but they do not align at all. Furthermore, my N-terminus *F aligns better with Anopheles gambiae AgGr22 where there is no possible *F N-terminal extension. My version is also in SWISSPROT and changing FlyBase *F would make the two agree. # *U FBrf0159830 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG31929 on Sat Jun 21 18:03:03 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 18:03:03 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG31929 on Sat Jun 21 18:03:03 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG31929 *F Release: 3 *F Gene annotation error *F Gene CG31929 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr22c *F ATGTTTGCATCACGTAGCGACTTGCAGTCCCGTCTGTGTTGGATTATTCTAAAGGCAACTTTGTACTCATCCTGGTTCC *F TTGGAGTATTTCCCTACAGATTCGATTCGCGAAATGGACAGCTGAAGCGCTCCAGATTTCTATTATTCTACGGACTAAT *F CTTGAACTTCTTCCTACTTCTGAAAATGGTGTGCTCA---------GGCGGTCAGAAACTGGGCATCCCAGAGGCATTT *F GCGCGGAATTCAGTTTTAGAAAATACACATTACACAACTGGCATGTTGGCCGTTTTCTCATGCGTGGTAATACATTTTT *F TGAACTTTTGGGGAAGCACAAGAGTGCAGGACTTGGCCAATGAATTGCTAGTCCTTGAGTATCAGCAATTTGCCAGTCT *F GAATGAAACGAAGTGCCCCAAGTTCAACTCTTTTGTGATTCAAAAGTGGCTTTCTGTCATTGGCCTTTTACTGTCATAT *F CTGAGTATAGCCTACGGACTGCCTGGCAACAACTTCTCCGTGGAAATGGTGCTCATAAACTCCTTAGTGCAATTCAGCT *F TCAATTGCAATATTATGCATTATTATATCGGAGTCTTGTTAATCTATCGCTATTTATGGCTGATCAACGGACAACTATT *F AGAAATGGTCACGAATCTTAAACTAGATTGTTCGGTGGACTCCTCGAGGATTCGGAAATACCTTTCATTGTACCGCCGT *F CTTTTGGAGCTCAAGGGATACATGGTGGCTACGTACGAGTACCATATGACCCTTGTTCTTACAACTGGCTTAGCGTCTA *F ATTTTTTGGCCATATATTCCTGGATTGTGTTGGACATCAGCATGAACATAAATTTCATCTATTTGCTAATTTTCCCACT *F GTTCTTACTTGTTAATGTTTGGAATCTTTGGTTGAGCATTGCCGCCAGCGATCTAGCTGAGAATGCTGGCAAAAGTACC *F CAAACTGTACTTAAACTTTTTGCGGATCTAGAGGTGAAAGA! *F TATTGAACTGGAGAGAAGTGTGAACGAATTCGCATTGCTGTGCGGTCATTGTCAGTTTAACTTTCACGTGTGTGGACT *F TTTCACTATAAACTATAAAATGGGTTTCCAGATGATCATCACCAGTTTTTTATACTTGATTTATATGATTCAATTCGAT *F TTCATGAATTTGTAA *F Protein sequence: *F >Gr22c *F MFASRSDLQSRLCWIILKATLYSSWFLGVFPYRFDSRNGQLKRSRFLLFYGLILNFFLLLKMVCSGGQKLGIPEAFARNS *F VLENTHYTTGMLAVFSCVVIHFLNFWGSTRVQDLANELLVLEYQQFASLNETKCPKFNSFVIQKWLSVIGLLLSYLSIAY *F GLPGNNFSVEMVLINSLVQFSFNCNIMHYYIGVLLIYRYLWLINGQLLEMVTNLKLDCSVDSSRIRKYLSLYRRLLELKG *F YMVATYEYHMTLVLTTGLASNFLAIYSWIVLDISMNINFIYLLIFPLFLLVNVWNLWLSIAASDLAENAGKSTQTVLKLF *F ADLEVKDIELERSVNEFALLCGHCQFNFHVCGLFTINYKMGFQMIITSFLYLIYMIQFDFMNL *F Comments: Again this is picky, but the D. pseudoobscura comparison shows that *F the intron donor splice site should be 6bp earlier than the current *F annotation, removing two aa from the protein. These two additional aa also do *F not align well. # *U FBrf0159831 *a Robertson *b H. *t 2003.6.21 *T personal communication to FlyBase *u FlyBase error report for CG13787 on Sat Jun 21 18:35:08 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 18:35:08 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13787 on Sat Jun 21 18:35:08 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13787 *F Release: 3 *F Gene annotation error *F Gene CG13787 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr28a *F ATGGCCTTTAAGTTGTGGGAGCGCTTTTCACAGGCGGACAATGTGTTCCAGGCACTTCGACCGCTAACATTCATATCGCT *F ATTGGGCCTGGCTCCATTTCGTTTGAATTTGAATCCTCGCAAGGAGGTGCAAACATCGAAGTTCTCCTTCTTTGCCGGCA *F TAGTGCACTTCCTGTTCTTCGTCCTGTGTTTTGGTATCTCCGTAAAGGAGGGAGATTCCATAATAGGGTACTTCTTTCAG *F ACCAATATCACCAGATTCAGCGATGGAACCCTACGCCTGACTGGCATCCTGGCCATGTCCACTATTTTTGGCTTTGCCAT *F GTTCAAGAGACAACGTTTGGTCAGCATAATACAGAACAACATAGTGGTGGACGAGATATTTGTGAGGCTGGGCATGAAGT *F TGGACTACCGCAGGATACTGTTGTCCAGCTTTCTCATATCCTTGGGCATGCTGCTGTTCAACGTCATTTACTTGTGTGTG *F AGCTACAGCCTGCTGGTCAGTGCCACCATATCGCCCTCATTTGTGACTTTCACAACCTTCGCCCTGCCGCACATCAATAT *F CAGCCTGATGGTCTTCAAGTTTCTTTGCACCACGGACTTGGCCAGGAGCCGGTTTAGTATGTTAAACGAAATCCTGCAGG *F ATATTTTGGATGCCCATATAGAGCAATTAAGCGCCTTGGAACTCTCACCCATGCACTCGGTTGTCAATCACAGACGCTAC *F TCACATCGCCTACGAAATTTGATTAGTACGCCAATGAAGCGGTACAGTGTTACCTCCGTCATACGCCTCAATCCCGAATA *F CGCAATCAAACAGGTGTCCAACATACACAATCTGCTCTGCGACATTTGCCAGACCATCGAGGAATACTTCACATATCCGC *F TGCTCGGAATCATAGCCATATCCTTTCTGTTCATTCTCTTTGATGACTTTTACATTTTGGAAGCCATTCTGAATCCCAAA *F CGACTGGATGTCTTTGAGGCCGATGAGTTCTTTGCCTTCTTCCTGATGCAGCTCATCTGGTATATAGTGATCATAGTGCT *F GATCGTGGAGGGCAGCAGTCGGACTATTTTGCATAGCAGCTATACGGCAGCTATAGTTCACAAGATTCTCAATATCACCG *F ATGATCCAGAACTCAGAGATCGACTTTTCCGTCTGTCCTTGCAGCTGTCGCATCGGAAGGTCCTTTTCACAGCCGCAGGA *F CTTTTTCGTCTGGATCGCACACTGATTTTTACGATTACTGGTGCTGCCACGTGCTACCTCATTATCCTAATTCAGTTTCG *F ATTCACACATCACATGGACGACACCAGCTCCAATTCAACAAATAATTTACATTCCATTCATCTCGGCGATTGA *F Protein sequence: *F >Gr28a *F MAFKLWERFSQADNVFQALRPLTFISLLGLAPFRLNLNPRKEVQTSKFSFFAGIVHFLFFVLCFGISVKEGDSIIGYFFQ *F TNITRFSDGTLRLTGILAMSTIFGFAMFKRQRLVSIIQNNIVVDEIFVRLGMKLDYRRILLSSFLISLGMLLFNVIYLCV *F SYSLLVSATISPSFVTFTTFALPHINISLMVFKFLCTTDLARSRFSMLNEILQDILDAHIEQLSALELSPMHSVVNHRRY *F SHRLRNLISTPMKRYSVTSVIRLNPEYAIKQVSNIHNLLCDICQTIEEYFTYPLLGIIAISFLFILFDDFYILEAILNPK *F RLDVFEADEFFAFFLMQLIWYIVIIVLIVEGSSRTILHSSYTAAIVHKILNITDDPELRDRLFRLSLQLSHRKVLFTAAG *F LFRLDRTLIFTITGAATCYLIILIQFRFTHHMDDTSSNSTNNLHSIHLGD *F Comments: Somehow the C-terminal exon is still missing from the annotation. It *F is correct in SWISSPROT. Comparisons with D. pseudoobscura and the rest of the *F family confirm it is needed to encode this 7TM receptor. # *U FBrf0159832 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG31747 on Sat Jun 21 19:13:43 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 19:13:43 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG31747 on Sat Jun 21 19:13:43 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG31747 *F Release: 3 *F Gene annotation error *F Gene CG31747 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr36a *F ATGTTTGACTGGGTCGGTTTGTTGTTAAAGGTACTCTACTACTATGGGCAGATCATTGGACTTATCAACTTCGAAATTGA *F CTGGCAAAGAGGTCGTGTCGTTGCAGCCCAAAGAGGCATTCTTTTCGCAATCGCAATTAACGTCTTAATTTGCATGGTGC *F TGCTTTTGCAAATATCCAAGAAATTCAATCTCGATGTGTACTTCGGTAGGGCTAACCAGCTGCATCAATATGTGATCATC *F GTGATGGTCTCACTGAGGATGGCTTCAGGAATTTCTGCAATTCTCAACAGATGGCGTCAGCGAGCACAACTAATGCGCCT *F TGTTGAATGTGTACTTCGGCTATTTCTGAAAAAACCGCATGTAAAGCAAATGTCCCGATGGGCAATTCTGGTAAAGTTCT *F CTGTAGGTGTCGTCAGCAATTTCCTACAAATGGCCATCTCTATGGAATCATTGGATCGCTTGGGGTTCAACGAATTCGTG *F GGAATGGCTTCGGATTTCTGGATGTCGGCCATTATAAATATGGCCATATCACAACACTATTTGGTAATACTTTTCGTTCG *F AGCCTATTACCATTTGCTCAAGACAGAGGTGCGGCAGGCGATCCATGAAAGCCAAATGTTAAGTGAGATTTACCCACGGA *F GAGCGGCTTTCATGACCAAGTGTTGTTACTTGGCTGATCGAATAGATAATATAGCAAAACTTCAGAATCAACTGCAATCG *F ATTGTGACCCAGTTGAACCAAGTGTTTGGCATCCAAGGGATAATGGTTTATGGCGGATACTATATATTCTCAGTAGCTAC *F AACTTACATAACGTACAGTTTAGCTATAAATGGTATAGAAGAACTGCACTTGAGTGTCAGAGCAGCGGCACTGGTATTTA *F GTTGGTTTTTATTCTACTACACGAGTGCAATACTAAATCTGTTTGTTATGCTCAAACTCTTCGATGATCACAAGGAGATG *F GAACGGATACTAGAAGAGAGAACTCTGTTTACTTCCGCCTTGGATGTCCGCTTGGAGCAATCCTTTGAAAGCATTCAATT *F GCAGCTAATTAGAAACCCGTTGAAAATTGAAGTATTGGATATATTTACCATTACTCGCAGTTCATCTGCTGCCATGATTG *F GATCTATAATAACGAATTCGATATTTCTTATTCAATACGATATGGAATATTTTTAA *F Protein sequence: *F >Gr36a *F MFDWVGLLLKVLYYYGQIIGLINFEIDWQRGRVVAAQRGILFAIAINVLICMVLLLQISKKFNLDVYFGRANQLHQYVI *F IVMVSLRMASGISAILNRWRQRAQLMRLVECVLRLFLKKPHVKQMSRWAILVKFSVGVVSNFLQMAISMESLDRLGFNE *F FVGMASDFWMSAIINMAISQHYLVILFVRAYYHLLKTEVRQAIHESQMLSEIYPRRAAFMTKCCYLADRIDNIAKLQNQ *F LQSIVTQLNQVFGIQGIMVYGGYYIFSVATTYITYSLAINGIEELHLSVRAAALVFSWFLFYYTSAILNLFVMLKLFDD *F HKEMERILEERTLFTSALDVRLEQSFESIQLQLIRNPLKIEVLDIFTITRSSSAAMIGSIITNSIFLIQYDMEYF *F Comments: There is an open in-frame intron that needs to be spliced near the *F C-terminus. Comparison with D. pseudoobscura confirms this, with a closed *F out-of-frame intron here. # *U FBrf0159833 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG30396 on Sat Jun 21 20:16:01 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 20:16:01 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG30396 on Sat Jun 21 20:16:01 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG30396 *F Release: 3 *F Gene annotation error *F Gene CG30396 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr58a *F ATGCTGTTGAAATTCATGTACATATATGGAATAGGCTGTGGTCTGATGCCTGCTCCTCTGAAGAAAGGACAGTTTCTTTT *F GGGATATAAGCAGAGATGGTACCTCATCTATACGGCCTGCCTGCACGGCGGTCTACTGACCGTCCTGCCCTTCACATTTC *F CCCACTACATGTACGACGACAGCTACATGAGCAGCAATCCAGTCCTGAAATGGACATTTAATCTAACCAACATCACCCGT *F ATTATGGCCATGTTTTCGGGCGTTCTCCTGATGTGGTTCAGAAGAAAAAGGATCTTGAATTTGGGTGAAAACTTAATACT *F TCACTGTCTTAAGTGCAAAACACTGGATAATCGTTCTAAGAAGTATTCTAAATTGCGGAAAAGAGTTCGAAATGTACTTT *F TCCAGATGTTACTCGTCGCAAATCTCAGCATTCTGCTGGGGGCTTTAATTTTGTTTAGAATTCATTCAGTACAAAGAATT *F AGTAAAACTGCAATGATTGTAGCCCATATTACACAGTTTATATATGTGGTCTTTATGATGACGGGAATATGTGTGATCCT *F ATTAGTTCTCCACTGGCAAAGTGAGAGACTTCAAATAGCACTCAAGGACTTGTGCTCCTTTCTTAATCATGAAGAACGCA *F ACTCCTTGACTTTATCAGAAAACAAGGCCAATAGATCCCTCGGAAAATTGGCTAAGCTCTTTAAACTTTTTGCGGAAAAT *F CAGCGATTAGTCCGAGAAGTTTTTCGAACCTTTGACTTGCCCATCGCCCTTCTTTTGCTGAAAATGTTTGTTACAAACGT *F GAATCTGGTTTATCATGGAGTGCAATTTGGCAACGATACCATAGAAACCTCAAGTTACACAAGAATCGTGGGACAGTGGG *F TGGTGATTTCTCATTACTGGAGTGCTGTTTTGCTCATGAATGTTGTGGATGACGTGACGCGGAGAAGTGACCTGAAAATG *F GGAGACCTCCTGCGAGAATTCAGTCATCTGGAGCTGGTCAAAAGGGACTTTCATTTGCAGCTGGAACTTTTCTCGGATCA *F CCTTCGTTGTCATCCGTCAACGTATAAGGTCTGTGGACTATTTATTTTCAATAAGCAAACGAGTTTGGCTTATTTTTTCT *F ATGTGCTGGTTCAAGTTTTGGTGCTTGTACAATTTGATTTAAAAAATAAAGTTGAAAAAAGAAATTAA *F Protein sequence: *F >Gr58a *F MLLKFMYIYGIGCGLMPAPLKKGQFLLGYKQRWYLIYTACLHGGLLTVLPFTFPHYMYDDSYMSSNPVLKWTFNLTNITR *F IMAMFSGVLLMWFRRKRILNLGENLILHCLKCKTLDNRSKKYSKLRKRVRNVLFQMLLVANLSILLGALILFRIHSVQRI *F SKTAMIVAHITQFIYVVFMMTGICVILLVLHWQSERLQIALKDLCSFLNHEERNSLTLSENKANRSLGKLAKLFKLFAEN *F QRLVREVFRTFDLPIALLLLKMFVTNVNLVYHGVQFGNDTIETSSYTRIVGQWVVISHYWSAVLLMNVVDDVTRRSDLKM *F GDLLREFSHLELVKRDFHLQLELFSDHLRCHPSTYKVCGLFIFNKQTSLAYFFYVLVQVLVLVQFDLKNKVEKRN *F Comments: Somehow this annotation did not get updated. It is correct in *F SWISSPROT and the D. pseudoobscura comparison confirms it. Needs longer *F N-terminus and a C-terminal intron/exon, like other genes in this family. # *U FBrf0159834 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG30189 on Sat Jun 21 20:28:55 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 20:28:55 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG30189 on Sat Jun 21 20:28:55 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG30189 *F Release: 3 *F Gene annotation error *F Gene CG30189 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr59a *F ATGAAGCGGATAGGTCAAGCGTATAACGTATACGCCGTGTTCATCGGCATGACGTCATACGAAACAATGGGTGGAAAATT *F CAGGCAGTCGCGGATTACTCGGATATATTGCTTGCTTATAAATGCCATCTTCTTGACTTTGCTACCGAGTGCTTTCTGGA *F AATCGGCCAAGCTTTTGAGTACGGCGGACTGGATGCCTTCCTACATGAGGGTCACTCCGTACATCATGTGTACAATCAAC *F TATGCTGCAATAGCCTACACCTTGATCTCAAGATGCTACAGGGACGCCATGTTGATGGACTTGCAACGCATTGTGCTGGA *F AGTGAATCGGGAAATGTTGCGCACGGGAAAGAAAATGAACTCACTACTTCGACGAATGTTTTTCTTGAAAACATTTACAT *F TGACCTACTCGTGCTTGTCGTACATTCTGGCGGTTTTCATTTACCAGTGGAAAGCTCAAAACTGGTCGAATCTTTGCAAT *F GGACTCTTGGTTAACATTTCCCTAACTATCCTGTTTGTCAACACATTCTTCTACTTCACCTCTTTGTGGCACATAGCCAG *F GGGATATGACTTTGTAAACCAGCAACTGAATGAGATTGTCGCCTGCCAATCGATGGACTTGGAGAGAAAGTCAAAAGAAC *F TCCGCGGCCTGTGGGCTCTGCACAGAAATCTTAGTTATACAGCACGCAGGATAAACAAGCACTACGGTCCCCAAATGCTT *F GCCATGCGATTTGATTACTTCATATTCTCCATCATCAACGCTTGCATAGGCACGATTTACTCGACTACCGACCAGGAGCC *F CTCGCTTGAAAAGATTTTCGGATCTCTAATTTACTGGGTGCGAAGTTTCGATTTCTTTCTGAACGACTATATCTGCGATC *F TGGTCAGCGAGTACCAAATGCAGCCAAAGTTCTTCGCCCCCGAAAGCAGCATGTCCAATGAGTTGAGTTCTTACTTGATC *F TACGAGAGCAGCACGCGATTGGATCTGTTGGTCTGCGGACTCTATCGTGTCAATAAGCGGAAATGGTTGCAAATGGTTGG *F CTCCATCGTAGTCCACTCAAGTATGCTGTTCCAGTTCCATCTCGTCATGCGAGGTGGTCTCTAG *F Protein sequence: *F >Gr59a *F MKRIGQAYNVYAVFIGMTSYETMGGKFRQSRITRIYCLLINAIFLTLLPSAFWKSAKLLSTADWMPSYMRVTPYIMCTI *F NYAAIAYTLISRCYRDAMLMDLQRIVLEVNREMLRTGKKMNSLLRRMFFLKTFTLTYSCLSYILAVFIYQWKAQNWSNL *F CNGLLVNISLTILFVNTFFYFTSLWHIARGYDFVNQQLNEIVACQSMDLERKSKELRGLWALHRNLSYTARRINKHYGP *F QMLAMRFDYFIFSIINACIGTIYSTTDQEPSLEKIFGSLIYWVRSFDFFLNDYICDLVSEYQMQPKFFAPESSMSNELS *F SYLIYESSTRLDLLVCGLYRVNKRKWLQMVGSIVVHSSMLFQFHLVMRGGL *F Comments: When CG13537 was split for some reason this annotation was not *F extended all the way to the appropriate start codon. Comparison with D. *F pseudoobscura and Gr59b and other family members confirm this version. # *U FBrf0159835 *a Robertson *b H. *t 2003.6.21 *T personal communication to FlyBase *u FlyBase error report for CG30330 on Sat Jun 21 20:36:40 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 20:36:40 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG30330 on Sat Jun 21 20:36:40 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG30330 *F Release: 3 *F Gene annotation error *F Gene CG30330 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr59d *F ATGGCTGACCTGCTCAAATTGTGTTTGAGAATCGCATATGCCTACGGACGGTTGACCGGCGTAATCAACTTTAAGATTGA *F TTTGAAAACGGGTCAAGCGCTAGTTACCAGAGGAGCTACGCTTATTTCAGTGAGCACACACTTGCTGATCTTTGCCCTAC *F TCCTCTACCAAACAATGCGAAAAAGTGTGGTGAACGTCATGTGGAAGTATGCCAATTCCCTTCACGAATACGTGTTCCTG *F GTCATAGCCGGCTTTCGCGTAGTCTGTGTCTTTCTGGAGCTGGTCAGTCGATGGTCGCAGCGTCGCACCTTTGTGAGGCT *F CTTCAACTCATTCCGGAGACTGTATCAGAGAAATCCGGATATAATCCAGTACTGCCGAAGGAGCATCGTTAGCAAATTCT *F TTTGCGTCACAATGACAGAGACACTGCACATCATAGTCACCTTGGCCATGATGAGGAATCGGCTGAGCATTGCTCTGGCT *F CTGCGCATTTGGGCCGTATTGAGTCTGACGGCCATAATAAATGTAATCATCACGCAGTACTATGTGGCCACCGCGTGTGT *F GCGAGGACGATATGCGCTCCTGAACAAGGATCTTCAGGCGATTGTGACCGAATCCCAGTCGCTGGTTCCCAACGGAGGTG *F GCGTCTTTGTGACCAAGTGTTGCTACCTAGCGGATCGCTTGGAGCGAATAGCCAAGTCCCAGTCGGACCTACAGGAGCTC *F GTCGAAAACTTGTCCACGGCATACGAAGGAGAAGTGGTCTGCCTGGTCATCACATACTATCTGAATATGCTGGGCACCTC *F GTATCTGCTGTTCAGCATTAGCAAGTATGGCAATTTTGGGAATAACCTGCTCGTGATCATCACTCTTTGTGGCATTGTCT *F ACTTCGTATTTTACGTCGTCGATTGCTGGATCAACGCGTTTAATGTGTTTTACCTTTTGGATGCCCATGATAAGATGGTT *F AAGTTGCTGAATAAGCGAACTTTGTTTCAGCCAGGTCTGGATCATCGATTGGAAATGGTTTTTGAAAACTTTGCTCTGAA *F CTTGGTGCGGAATCCATTGAAGCTCCATATGTACGGCCTTTTCGAGTTTGGTCGAGGAACATCCTTTGCCGTGTTTAACT *F CCCTGTTAACACACTCCCTTCTCCTCATTCAATACGACGTGCAAAACTTCTAA *F Protein sequence: *F >Gr59d *F MADLLKLCLRIAYAYGRLTGVINFKIDLKTGQALVTRGATLISVSTHLLIFALLLYQTMRKSVVNVMWKYANSLHEYVFL *F VIAGFRVVCVFLELVSRWSQRRTFVRLFNSFRRLYQRNPDIIQYCRRSIVSKFFCVTMTETLHIIVTLAMMRNRLSIALA *F LRIWAVLSLTAIINVIITQYYVATACVRGRYALLNKDLQAIVTESQSLVPNGGGVFVTKCCYLADRLERIAKSQSDLQEL *F VENLSTAYEGEVVCLVITYYLNMLGTSYLLFSISKYGNFGNNLLVIITLCGIVYFVFYVVDCWINAFNVFYLLDAHDKMV *F KLLNKRTLFQPGLDHRLEMVFENFALNLVRNPLKLHMYGLFEFGRGTSFAVFNSLLTHSLLLIQYDVQNF *F Comments: A strange minor problem with the intron donor site, which needs to *F be slightly later, keeping two extra aa in protein. Comparison with D. *F pseudoobscura confirms this version. # *U FBrf0159836 *a Robertson *b H. *t 2003.6.21 *T personal communication to FlyBase *u FlyBase error report for CG7189 on Sat Jun 21 23:20:12 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 23:20:12 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG7189 on Sat Jun 21 23:20:12 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG7189 *F Release: 3 *F Gene annotation error *F Gene CG7189 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr66a *F ATGGACAACATGGCGCAGGCGGAGGACGCAGTGCAACCACTATTGCAGCAGTTCCAGCAACTGTTCTTCATATCCAAGAT *F AGCTGGAATTCTGCCACAGGATCTCGAGAAGTTTCGATCTAGGAATCTGCTGGAGAAATCCCGTAATGGCATGATTTACA *F TGCTGAGTACTTTAATACTCTACGTTGTGCTCTATAATATTTTGATATATTCCTTTGGAGAGGAGGACCGCAGCCTAAAG *F GCCTCGCAGAGCACCTTGACTTTCGTGATTGGCTTGTTCCTGACCTATATCGGTCTGATTATGATGGTCTCCGACCAGTT *F GACCGCGTTACGAAACCAGGGTAGAATTGGAGAGCTTTACGAGCGCATCCGTCTGGTGGATGAGCGCCTTTACAAAGAGG *F GGTGTGTTATGGACAACAGTACAATTGGACGGCGCATACGAATTATGCTGATCATGACGGTCATCTTTGAGTTGTCCATT *F TTGGTGAGCACCTATGTCAAGCTGGTGGACTATAGTCAATGGATGTCCTTGTTATGGATAGTGTCCGCCATTCCCACGTT *F CATCAACACGCTAGACAAGATCTGGTTCGCTGTTTCGTTATATGCGTTGAAAGAACGCTTCGAGGCCATAAACGCCACCC *F TAGAGGAACTGGTGGACACGCACGAGAAGCATAAGCTGTGGCTGCGAGGCAATCAAGAGGTTCCGCCTCCTCTGGACAGC *F TCCCAGCCGCCTCAGTATGACAGCAACCTGGAGTATCTGTACAAGGAACTAGGAGGTATGGACATAGGTTCCATTGGCAA *F GAGTTCAGTGTCTGGTTCGGGGAAAAACAAAGTAGCACCAGTGGCCCACTCCATGAACTCCTTTGGTGAAGCAATTGATG *F CGGCCAGCAGGAAGCCTCCACCGCCTCCCCTGGCCACTAACATGGTCCATGAAAGCGAGCTGGGAAATGCCGCTAAGGTA *F GAGGAGAAACTAAACAACCTGTGCCAGGTGCACGACGAGATCTGTGAAATCGGAAAAGCTTTGAACGAGCTGTGGAGCTA *F TCCCATTCTATCTCTAATGGCCTATGGTTTTCTGATTTTCACTGCTCAACTTTATTTCCTCTACTGCGCTACACAGTACC *F AATCGATACCATCGCTTTTCCGTTCCGCCAAGAATCCCTTCATCACTGTTATAGTTCTAAGTTATACGTCTGGAAAATGC *F GTGTACCTCATCTACCTGAGTTGGAAAACGTCGCAGGCCTCCAAGCGCACAGGAATCAGTCTGCACAAATGTGGCGTGGT *F GGCCGATGATAATCTTCTCTACGAAATTGTTAACCACCTATCGCTAAAATTGCTCAACCACTCGGTGGACTTTTCGGCTT *F GCGGCTTCTTTACCCTGGACATGGAAACATTGTATGGTGTGAGTGGCGGGATCACTAGCTACCTGATCATCCTGATTCAG *F TTCAATTTGGCCGCCCAGCAGGCCAAAGAGGCTATACAGACGTTCAACTCGCTTAATGACACCGCCGGCTTGGTTGGTGC *F CGCCACCGATATGGATAATATTAGCTCCACGCTGCGTGATTTCGTCACCACGACCATGACACCGGCGGTCTAA *F Protein sequence: *F >Gr66a *F MDNMAQAEDAVQPLLQQFQQLFFISKIAGILPQDLEKFRSRNLLEKSRNGMIYMLSTLILYVVLYNILIYSFGEEDRSLK *F ASQSTLTFVIGLFLTYIGLIMMVSDQLTALRNQGRIGELYERIRLVDERLYKEGCVMDNSTIGRRIRIMLIMTVIFELSI *F LVSTYVKLVDYSQWMSLLWIVSAIPTFINTLDKIWFAVSLYALKERFEAINATLEELVDTHEKHKLWLRGNQEVPPPLDS *F SQPPQYDSNLEYLYKELGGMDIGSIGKSSVSGSGKNKVAPVAHSMNSFGEAIDAASRKPPPPPLATNMVHESELGNAAKV *F EEKLNNLCQVHDEICEIGKALNELWSYPILSLMAYGFLIFTAQLYFLYCATQYQSIPSLFRSAKNPFITVIVLSYTSGKC *F VYLIYLSWKTSQASKRTGISLHKCGVVADDNLLYEIVNHLSLKLLNHSVDFSACGFFTLDMETLYGVSGGITSYLIILIQ *F FNLAAQQAKEAIQTFNSLNDTAGLVGAATDMDNISSTLRDFVTTTMTPAV *F Comments: Comparison with D. pseudoobscura suggests that the second phase 1 *F intron, which is open and in frame, is not real. It encodes amino acids that *F align well with those encoded by Dp. Remarkably the Dp gene has a unique phase *F 1 intron of its own (for real with stop codons and a frameshift) in this region. # *U FBrf0159837 *a Robertson *b H. *t 2003.6.21 *T personal communication to FlyBase *u FlyBase error report for CG31208 on Sat Jun 21 23:37:12 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 23:37:12 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG31208 on Sat Jun 21 23:37:12 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG31208 *F Release: 3 *F Gene annotation error *F Gene CG31208 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr92a *F ATGTTCGAGTTCCTTCATCAGATGAGTGCACCGAAACTATCCACCTCAATTTTGAGATACATTTTTCGGTACGCGCAATT *F TATTGGAGTGATCTTTTTCTGTCTACACACTCGAAAGGACGATAAAACTGTATTTATTCGCAATTGGCTCAAGTGGCTGA *F ATGTTACGCATCGTATAATAACTTTTACCAGATTCTTTTGGGTCTATATAGCGTCCATATCGATTAAGACAAATCGCGTC *F CTACAAGTTCTTCATGGTATGCGACTTGTGCTGAGCATTCCCAACGTTGCAGTTATCTTGTGCTACCACATTTTTCGAGG *F GCCAGAAATAATCGATCTTATCAACCAGTTTTTGCGTCTCTTTCGGCAAGTGAGTGATTTATTCAAGACGAAAACACCTG *F GCTTTGGCGGCAGACGAGAACTGATATTAATCCTACTGAATTTGATATCCTTCGCCCATGAGCAAACATATCTGTGGTTT *F ACGATACGGAAAGGATTTTCTTGGCGTTTCTTAATCGATTGGTGGTGCGACTTTTACCTAGTCAGTGCCACCAATATATT *F CATCCACATCAATTCCATCGGCTATCTGAGTCTGGGTGTTCTTTATTCCGAGCTCAATAAATATGTATATACAAACTTGC *F GAATTCAATTGCAAAAATTAAATACTTCGGGCAGTAAACAAAAAATACGAAGAGTGCAAAATCGCTTGGAAAAATGCATA *F AGCTTGTACAGAGAGATATATCATACCAGCATTATGTTTCACAAGCTATTTGTTCCTCTCCTTTTCCTGGCATTGATTTA *F CAAGGTTTTACTAATTGCCTTGATTGGCTTTAATGTAGCTGTAGAATTTTATTTAAACAGCTTCATATTCTGGATACTTT *F TGGGCAAACACGTATTGGATCTTTTTCTGGTAACTGTCTCCGTTGAGGGAGCGGTTAACCAATTTTTGAACATTGGAATG *F CAGTTTGGAAATGTCGGTGATCTCAGTAAATTTCAAACTACGCTGGATACTTTATTCCTTCATCTACGACTTGGCCATTT *F TCGAGTCAGCATTTTGGGCCTATTCGATGTCACCCAAATGCAATATTTACAGTTCTTATCAGCACTCCTATCTGGGCTAG *F CTTTTATAGCACAATATAGAATGCAGGTTGGAAACGGTTAG *F Protein sequence: *F >Gr92a *F MFEFLHQMSAPKLSTSILRYIFRYAQFIGVIFFCLHTRKDDKTVFIRNWLKWLNVTHRIITFTRFFWVYIASISIKTNRV *F LQVLHGMRLVLSIPNVAVILCYHIFRGPEIIDLINQFLRLFRQVSDLFKTKTPGFGGRRELILILLNLISFAHEQTYLWF *F TIRKGFSWRFLIDWWCDFYLVSATNIFIHINSIGYLSLGVLYSELNKYVYTNLRIQLQKLNTSGSKQKIRRVQNRLEKCI *F SLYREIYHTSIMFHKLFVPLLFLALIYKVLLIALIGFNVAVEFYLNSFIFWILLGKHVLDLFLVTVSVEGAVNQFLNIGM *F QFGNVGDLSKFQTTLDTLFLHLRLGHFRVSILGLFDVTQMQYLQFLSALLSGLAFIAQYRMQVGNG *F Comments: This gene clearly needs an intron and exon at the end to encode the *F TM7 region of these 7TM gustatory receptors. Although the Dp ortholog has been *F lost, comparison with the rest of the family shows this clearly. # *U FBrf0159838 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG11767 on Sun Jun 22 20:11:18 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sun, 22 Jun 2003 20:11:18 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG11767 on Sun Jun 22 20:11:18 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG11767 *F Release: 3 *F Gene annotation error *F Gene CG11767 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Or24a *F ATGGAGCGCCATTATTTCATGGTGCCAAAGTTTGCATTATCGCTGATTGGTTTTTATCCCGAACAGAAGCGAACGGTTTT *F GGTGAAACTTTGGAGTTTCTTCAACTTTTTCATCCTCACCTACGGCTGTTATGCAGAGGCTTACTATGGCATACACTATA *F TACCGATTAACATAGCCACTGCATTGGATGCCCTTTGTCCTGTGGCCTCCAGCATTTTGTCGCTGGTGAAAATGGTCGCC *F ATTTGGTGGTATCAAGATGAATTAAGGAGTTTGATAGAGCGGGTAAGATTTTTAACAGAGCAACAGAAGTCCAAGAGGAA *F ACTGGGCTATAAGAAGAGGTTCTATACACTGGCAACGCAACTAACATTCCTGCTACTATGCTGTGGATTTTGCACCAGTA *F CTTCCTATTCCGTCAGACATTTGATTGATAATATCCTGAGACGCACCCATGGCAAGGACTGGATCTACGAGACTCCGTTC *F AAGATGATGTTCCCCGATCTTCTCCTGCGTTTGCCACTCTATCCCATCACCTATATACTCGTGCATTGGCATGGCTACAT *F TACTGTGGTTTGTTTTGTCGGCGCGGATGGTTTCTTCCTGGGGTTCTGTTTGTACTTCACTGTTTTGCTGCTCTGTCTGC *F AGGACGATGTTTGTGATTTACTAGAGGTTGAAAACATCGAGAAGAGTCCCTCCGAAGCGGAGGAAGCTCGCATAGTTCGG *F GAAATGGAAAAACTGGTGGACCGGCATAACGAGGTGGCCGAGCTGACAGAAAGATTGTCGGGTGTTATGGTGGAAATAAC *F ACTGGCCCACTTTGTTACTTCGAGTTTGATAATCGGAACCAGCGTGGTGGATATTTTATTATTTTCCGGCCTGGGAATCA *F TTGTGTATGTGGTCTACACTTGTGCCGTAGGTGTGGAAATATTTCTATACTGTTTAGGAGGATCTCATATTATGGAAGCG *F TGTTCCAATCTAGCGCGCTCCACATTTTCCAGCCACTGGTATGGCCACAGTGTTCGGGTCCAAAAGATGACCCTTTTGAT *F GGTAGCTCGTGCTCAACGAGTTCTCACAATTAAAATTCCTTTCTTTTCCCCATCATTAGAGACTCTAACTTCGATTTTGC *F GCTTCACTGGATCTCTGATTGCCCTGGCAAAGTCGGTTATATAA *F Protein sequence: *F >Or24a *F MERHYFMVPKFALSLIGFYPEQKRTVLVKLWSFFNFFILTYGCYAEAYYGIHYIPINIATALDALCPVASSILSLVKMVA *F IWWYQDELRSLIERVRFLTEQQKSKRKLGYKKRFYTLATQLTFLLLCCGFCTSTSYSVRHLIDNILRRTHGKDWIYETPF *F KMMFPDLLLRLPLYPITYILVHWHGYITVVCFVGADGFFLGFCLYFTVLLLCLQDDVCDLLEVENIEKSPSEAEEARIVR *F EMEKLVDRHNEVAELTERLSGVMVEITLAHFVTSSLIIGTSVVDILLFSGLGIIVYVVYTCAVGVEIFLYCLGGSHIMEA *F CSNLARSTFSSHWYGHSVRVQKMTLLMVARAQRVLTIKIPFFSPSLETLTSILRFTGSLIALAKSVI *F Comments: The current annotation has an extra intron near the N-terminus that *F is open and in frame. Comparison with the rest of the Or family, and the D. *F pseudoobscura ortholog strongly suggests that this intron is not real and this *F coding sequence should be used. # *U FBrf0159839 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG13206 on Sun Jun 22 21:00:55 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sun, 22 Jun 2003 21:00:55 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13206 on Sun Jun 22 21:00:55 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13206 *F Release: 3 *F Gene annotation error *F Gene CG13206 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Or47b *F ATGAACGACTCGGGTTATCAATCAAATCTCAGCCTTCTGCGGGTTTTTCTCGACGAGTTCCGATCGGTTCTGCGGCAGGA *F AAGTCCCGGTCTCATCCCACGCCTGGCTTTTTACTATGTTCGCGCCTTTCTGAGTTTGCTGTGCCAGTATCCCAACAAGA *F AGTTGGCCAGCTTGCCCCTGTACCGATGGATCAACTTGTTCATCATGTGCAATGTGATGACCATTTTCTGGACCATGTTC *F GTGGCCCTGCCCGAGTCGAAGAACGTGATCGAAATGGGCGACGACTTGGTTTGGATTTCGGGGATGGCACTGGTGTTCAC *F CAAGATCTTTTACATGCATTTGCGTTGCGACGAGATCGATGAACTTATTTCGGATTTTGAATACTACAACCGGGAGCTGA *F GACCCCATAATATCGATGAGGAGGTGTTGGGTTGGCAGAGACTGTGCTACGTGATAGAATCGGGTCTATATATCAACTGC *F TTTTGCCTGGTCAACTTCTTCAGTGCCGCTATTTTCCTGCAACCTCTGTTGGGCGAGGGAAAGCTGCCCTTCCACAGCGT *F CTATCCGTTTCAATGGCATCGCTTGGATCTGCATCCCTACACGTTCTGGTTCCTCTACATCTGGCAGAGTCTGACCTCGC *F AGCACAACCTAATGAGCATTCTAATGGTGGATATGGTAGGCATTTCCACGTTCCTCCAGACGGCGCTCAATCTCAAGTTG *F CTTTGCATCGAGATAAGGAAACTGGGGGACATGGAGGTCAGTGATAAGAGGTTCCACGAGGAGTTTTGTCGTGTGGTTCG *F CTTCCACCAGCACATTATCAAATTGGTGGGGAAAGCCAATAGAGCTTTCAATGGCGCCTTCAATGCACAATTAATGGCCA *F GTTTCTCCCTGATTTCCATATCCACTTTCGAGACCATGGCTGCAGCGGCTGTGGATCCCAAAATGGCCGCCAAGTTCGTG *F CTTCTCATGCTGGTGGCATTCATTCAACTGTCGCTTTGGTGCGTCTCTGGAACTTTGGTTTATACTCAGTCAGTGGAGGT *F GGCTCAGGCTGCTTTTGATATCAACGATTGGCACACCAAATCGCCAGGCATCCAGAGGGATATATCCTTTGTGATACTAC *F GAGCCCAGAAACCCCTGATGTATGTGGCCGAACCATTTCTGCCCTTCACCCTGGGAACCTATATGCTTGTTCTGAAGAAC *F TGCTATCGTTTGCTGGCCCTGATGCAAGAATCGATGTAG *F Protein sequence: *F >Or47b *F MNDSGYQSNLSLLRVFLDEFRSVLRQESPGLIPRLAFYYVRAFLSLLCQYPNKKLASLPLYRWINLFIMCNVMTIFWTMF *F VALPESKNVIEMGDDLVWISGMALVFTKIFYMHLRCDEIDELISDFEYYNRELRPHNIDEEVLGWQRLCYVIESGLYINC *F FCLVNFFSAAIFLQPLLGEGKLPFHSVYPFQWHRLDLHPYTFWFLYIWQSLTSQHNLMSILMVDMVGISTFLQTALNLKL *F LCIEIRKLGDMEVSDKRFHEEFCRVVRFHQHIIKLVGKANRAFNGAFNAQLMASFSLISISTFETMAAAAVDPKMAAKFV *F LLMLVAFIQLSLWCVSGTLVYTQSVEVAQAAFDINDWHTKSPGIQRDISFVILRAQKPLMYVAEPFLPFTLGTYMLVLKN *F CYRLLALMQESM *F Comments: Comparison with D. pseudoobscura shows that the first intron should *F be shorter. Specifically, the acceptor site used now is not present in Dp, *F instead the earlier one I use is present and the exon encodes conserved aa. # *U FBrf0159840 *a Hultmark *b D. *t 2003.6.23 *T personal communication to FlyBase *u FlyBase error report for CG4432 on Mon Jun 23 08:02:20 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 23 Jun 2003 08:02:20 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: dan.hultmark@ucmp.umu.se *F Subject: FlyBase error report for CG4432 on Mon Jun 23 08:02:20 2003 *F Error report from Dan Hultmark (dan.hultmark@ucmp.umu.se) *F Gene or accession: CG4432 *F Release: 3 *F Gene annotation error *F Gene CG4432 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >PGRP-LC transcript, splice form x *F GTCACTCGAACGCGCCGCTGCTCAACGATTCGAAATTGGCGCGAAACGGTATAAAAAGTTCGACTTACAAATTGGCTATA *F TAGTATAGACGCTCAAATATCAAACGAGCTGCAACGGTTTTCGTCGGCAATTTAAAATGCTCATGATGTGATGTTTGCCG *F AGAGCTGTGCCTAGTCAGCCGTTTTGCCATACGAAAAATAATGTACCGCCCAATCTGCTAAATAGGAAGGCTAACACATA *F ACGCTCAGAGATCCAGAGAAAATATCAATCTGGTTACGTGACTGTCACGATAGTTCGACATGCCTTTTAGCAATGAAACG *F GAAATGAGCCAATGTTCCAATGCGAAACGTAGAGTAAACGATCCGTTGACTGGACCAAAAAACTGCAGTACTTCGTCCAC *F AGACTCGGGCGTTATACTAAACGATAATGTGGCGGCATTCCGGCCGGAGAAGGAGACCAAAGATCGGGGGACGGGCGAAG *F GGCAGTTCCAATCGAAATCGGAAGAGAAAACGGAATCCAAGCGTATCAGCGTTGAGCACACTGTCAATATAACAACGGAA *F AATGTTGGAAAGACATCTTCGCCGGCGGTTTCCATACGGAGTACCACAATTTCCGTTGTGTCCATCGATGATAATGCCAT *F CGACTCGAGTAGTATTGATAGTGATTCGGAGGCCGAAGCGGAGGATTATACGGTCCAGAAGCTGGGTCACCAGGTCACCT *F ACCCGCCCAACAGTTCACACCTGCGCGACCTTAACCAGGGTCTAACGGTGATCAGTCGCCATGTGGCGCCAGGTGAGGCG *F GCAGTACCACCACCCAATCCCCTGGAAGCTGGCATTGTGGCCAAGCAAATACTGAACGGTAATCTGGCCGTGGCCACGCC *F CACATCGCCGGCGGGCGGTGCCACGCAGGGTATTGGCAGCATCGCCCTGACCAACTCCACAGATGTGACGTTCGGTGATA *F AGCATTTCTACGAGGGACCCGTGACGATACAACAGTTTCTCATCGATAATCGGGACAAGTGGAAACCGGGCGAGGGACCA *F GCTGGTGGACAGGATAACCCCGCATTCAATGGTGGTCCCAGCACGAATGGAAGTGCGCCGGGCTCCAAACACGAAGATCC *F GGCGCAAACCCCACCAATTTGTCCTTTTCTGCCCAACACTGTCGGACGCAAGGCCGTCACAGTTACAGTGGTTTTTGTAA *F CTTTAACCTTCCTGCTGGGTATCGTACTGGCCACCACCACAAATCTCTTCGGAAAGACGTTGAACCAAACGGACTTGGAT *F GTCATCGATAATAGCACATTGGTGATCCTAAAAGTAGCCGAGTGGGGCGGAAGACCTGCCAAGAGGATGTTGGATGCGCA *F ACAGTTGCCCATCAATCGGGTGGTCATCTCGCACACCGCCGCTGAGGGTTGCGAATCGAGGGAAGTCTGCTCCGCCCGGG *F TGAATGTCGTCCAATCGTTTCACATGGACAGCTGGGGTTGGGACCACATAGGCTACAACTTTCTCGTGGGCGGCGATGGC *F CGTGTGTACGAGGGGCGTGGCTGGGACTATGTGGGCGCTCACACCAAGGGTTATAATAGAGGAAGCATTGGTATATCCTT *F TATTGGAACCTTCACGACAAGAAAACCCAACGAACGGCAGTTAGAGGCATGCCAACTGCTTTTGCAAGAGGGCGTTCGTC *F TTAAGAAATTGACGACGAATTATCGTCTTTATGGCCATCGTCAACTGAGTGCCACGGAGAGTCCCGGGGAGGAGCTCTAC *F AAGATCATTAAAAAGTGGCCGCACTGGTCACACGAAATCTAATAAGTCATGTATTATATAAAAATTACAAGTAAATAGAG *F TCTTAAGAAATATG *F >PGRP-LC transcript, splice form y *F GTCACTCGAACGCGCCGCTGCTCAACGATTCGAAATTGGCGCGAAACGGTATAAAAAGTTCGACTTACAAATTGGCTATA *F TAGTATAGACGCTCAAATATCAAACGAGCTGCAACGGTTTTCGTCGGCAATTTAAAATGCTCATGATGTGATGTTTGCCG *F AGAGCTGTGCCTAGTCAGCCGTTTTGCCATACGAAAAATAATGTACCGCCCAATCTGCTAAATAGGAAGGCTAACACATA *F ACGCTCAGAGATCCAGAGAAAATATCAATCTGGTTACGTGACTGTCACGATAGTTCGACATGCCTTTTAGCAATGAAACG *F GAAATGAGCCAATGTTCCAATGCGAAACGTAGAGTAAACGATCCGTTGACTGGACCAAAAAACTGCAGTACTTCGTCCAC *F AGACTCGGGCGTTATACTAAACGATAATGTGGCGGCATTCCGGCCGGAGAAGGAGACCAAAGATCGGGGGACGGGCGAAG *F GGCAGTTCCAATCGAAATCGGAAGAGAAAACGGAATCCAAGCGTATCAGCGTTGAGCACACTGTCAATATAACAACGGAA *F AATGTTGGAAAGACATCTTCGCCGGCGGTTTCCATACGGAGTACCACAATTTCCGTTGTGTCCATCGATGATAATGCCAT *F CGACTCGAGTAGTATTGATAGTGATTCGGAGGCCGAAGCGGAGGATTATACGGTCCAGAAGCTGGGTCACCAGGTCACCT *F ACCCGCCCAACAGTTCACACCTGCGCGACCTTAACCAGGGTCTAACGGTGATCAGTCGCCATGTGGCGCCAGGTGAGGCG *F GCAGTACCACCACCCAATCCCCTGGAAGCTGGCATTGTGGCCAAGCAAATACTGAACGGTAATCTGGCCGTGGCCACGCC *F CACATCGCCGGCGGGCGGTGCCACGCAGGGTATTGGCAGCATCGCCCTGACCAACTCCACAGATGTGACGTTCGGTGATA *F AGCATTTCTACGAGGGACCCGTGACGATACAACAGTTTCTCATCGATAATCGGGACAAGTGGAAACCGGGCGAGGGACCA *F GCTGGTGGACAGGATAACCCCGCATTCAATGGTGGTCCCAGCACGAATGGAAGTGCGCCGGGCTCCAAACACGAAGATCC *F GGCGCAAACCCCACCAATTTGTCCTTTTCTGCCCAACACTGTCGGACGCAAGGCCGTCACAGTTACAGTGGTTTTTGTAA *F CTTTAACCTTCCTGCTGGGTATCGTACTGGCCACCACCACAAATCTCTTCGGAAAGACGTTGAACCAAACGAATGATCCT *F AAAATCCATGTGGATGGAAAACTAGTTGTGGTCAGTATAAAAGGCTGGGGTGGTATGCCTACTAGAGGAAACCTAAAGCC *F ATTTAAACTTCCGGTCTCCAAGGTAATTATCTCTGAGACTCCACCCGAGATATGTACGACACAGGATTCCTGTTCTTATT *F GGACTCGAGTAACCCAAAGCAGGCACATGGATACCTTCAACTGGAGTCAAGTGGGATATAATTTTCTTGTGGGCGGAGAT *F GGACGTATTTACGAGGGGCGTGGCTGGAACTACATGGGCGATCATACGAGGGACAATAATAACAACAGTATCGGTATTAC *F ATTTTTGGGAACATTTCGACGACAGGAGCCGACGCCAAAATCATTAGAGGCATGTCAGCTGCTCATCGCTCAAGGTGTTC *F GCCTCAAGAAACTAAAGCCAGATTACCAACTGCTGGGACATCGGCAAATCACAGGCACTTTGATGCCCGGCGAGGAACTC *F TATAGAATCATTCAAACCTGGAATAACTGGTACAATCTGACGAAAACTTGGCCCGATCTGCATATGACTCAATAGTTATA *F ATTAATACAATCAAGAACGAATAAAGGTGTTCAAAAAATG *F >PGRP-LC transcript, splice form a (corresponds to CG4432-RB) *F GTCACTCGAACGCGCCGCTGCTCAACGATTCGAAATTGGCGCGAAACGGTATAAAAAGTTCGACTTACAAATTGGCTATA *F TAGTATAGACGCTCAAATATCAAACGAGCTGCAACGGTTTTCGTCGGCAATTTAAAATGCTCATGATGTGATGTTTGCCG *F AGAGCTGTGCCTAGTCAGCCGTTTTGCCATACGAAAAATAATGTACCGCCCAATCTGCTAAATAGGAAGGCTAACACATA *F ACGCTCAGAGATCCAGAGAAAATATCAATCTGGTTACGTGACTGTCACGATAGTTCGACATGCCTTTTAGCAATGAAACG *F GAAATGAGCCAATGTTCCAATGCGAAACGTAGAGTAAACGATCCGTTGACTGGACCAAAAAACTGCAGTACTTCGTCCAC *F AGACTCGGGCGTTATACTAAACGATAATGTGGCGGCATTCCGGCCGGAGAAGGAGACCAAAGATCGGGGGACGGGCGAAG *F GGCAGTTCCAATCGAAATCGGAAGAGAAAACGGAATCCAAGCGTATCAGCGTTGAGCACACTGTCAATATAACAACGGAA *F AATGTTGGAAAGACATCTTCGCCGGCGGTTTCCATACGGAGTACCACAATTTCCGTTGTGTCCATCGATGATAATGCCAT *F CGACTCGAGTAGTATTGATAGTGATTCGGAGGCCGAAGCGGAGGATTATACGGTCCAGAAGCTGGGTCACCAGGTCACCT *F ACCCGCCCAACAGTTCACACCTGCGCGACCTTAACCAGGGTCTAACGGTGATCAGTCGCCATGTGGCGCCAGGTGAGGCG *F GCAGTACCACCACCCAATCCCCTGGAAGCTGGCATTGTGGCCAAGCAAATACTGAACGGTAATCTGGCCGTGGCCACGCC *F CACATCGCCGGCGGGCGGTGCCACGCAGGGTATTGGCAGCATCGCCCTGACCAACTCCACAGATGTGACGTTCGGTGATA *F AGCATTTCTACGAGGGACCCGTGACGATACAACAGTTTCTCATCGATAATCGGGACAAGTGGAAACCGGGCGAGGGACCA *F GCTGGTGGACAGGATAACCCCGCATTCAATGGTGGTCCCAGCACGAATGGAAGTGCGCCGGGCTCCAAACACGAAGATCC *F GGCGCAAACCCCACCAATTTGTCCTTTTCTGCCCAACACTGTCGGACGCAAGGCCGTCACAGTTACAGTGGTTTTTGTAA *F CTTTAACCTTCCTGCTGGGTATCGTACTGGCCACCACCACAAATCTCTTCGGAAAGACGTTGAACCAAAGTAAGATCAGA *F GATGACGACGATTACAGACAAAATATTCCAATCAATTCAACAATAGATCTGGACAACATTGGTGGTGGACTAATACTGAG *F ATTCGTGGAGCGTCAGCAATGGCTCGCACAGCCGCCACAGAAGGAGATTCCCGATCTGGAGCTGCCCGTGGGATTGGTCA *F TAGCCTTGCCCACCAATTCAGAAAATTGCAGCACACAGGCGATCTGTGTGCTCCGAGTTCGTCTGCTGCAGACCTACGAT *F ATAGAATCGTCGCAGAAGTGTGATATTGCGTACAACTTTCTGATTGGCGGAGATGGAAATGTATATGTGGGACGGGGCTG *F GAATAAAATGGGTGCCCACATGAACAATATAAACTATGACTCGCAGAGTCTTAGCTTCGCCTATATCGGATCGTTTAAGA *F CCATCCAGCCGTCGGCCAAGCAACTAAGTGTTACTCGTCTTTTGCTGGAGCGTGGCGTAAAGCTGGGCAAGATTGCACCA *F AGCTACCGATTTACGGCGAGCAGTAAATTGATGCCAAGTGTAACCGATTTTAAGGCGGATGCGCTCTACGCAAGCTTCGC *F CAACTGGACTCATTGGTCGTGAATAGGTTAAGAGCTATTTCGATATTTTGCAATACTATCATTTATTTTCGTATTGATTT *F TGTATCATAGGTGTTCTCCCATTAATGTGTGGCATATTCGATTTAGCTAGAGTAACGATTCGTTTAAAAAGAAAATGTGA *F TATTAAATAAAACAAAATGATTT *F Protein sequence: *F >PGRP-LC transcript, splice form x *F MPFSNETEMSQCSNAKRRVNDPLTGPKNCSTSSTDSGVILNDNVAAFRPEKETKDRGTGEGQFQSKSEEKTESKRISVEH *F TVNITTENVGKTSSPAVSIRSTTISVVSIDDNAIDSSSIDSDSEAEAEDYTVQKLGHQVTYPPNSSHLRDLNQGLTVISR *F HVAPGEAAVPPPNPLEAGIVAKQILNGNLAVATPTSPAGGATQGIGSIALTNSTDVTFGDKHFYEGPVTIQQFLIDNRDK *F WKPGEGPAGGQDNPAFNGGPSTNGSAPGSKHEDPAQTPPICPFLPNTVGRKAVTVTVVFVTLTFLLGIVLATTTNLFGKT *F LNQTDLDVIDNSTLVILKVAEWGGRPAKRMLDAQQLPINRVVISHTAAEGCESREVCSARVNVVQSFHMDSWGWDHIGYN *F FLVGGDGRVYEGRGWDYVGAHTKGYNRGSIGISFIGTFTTRKPNERQLEACQLLLQEGVRLKKLTTNYRLYGHRQLSATE *F SPGEELYKIIKKWPHWSHEI *F >PGRP-LC transcript, splice form y *F MPFSNETEMSQCSNAKRRVNDPLTGPKNCSTSSTDSGVILNDNVAAFRPEKETKDRGTGEGQFQSKSEEKTESKRISVEH *F TVNITTENVGKTSSPAVSIRSTTISVVSIDDNAIDSSSIDSDSEAEAEDYTVQKLGHQVTYPPNSSHLRDLNQGLTVISR *F HVAPGEAAVPPPNPLEAGIVAKQILNGNLAVATPTSPAGGATQGIGSIALTNSTDVTFGDKHFYEGPVTIQQFLIDNRDK *F WKPGEGPAGGQDNPAFNGGPSTNGSAPGSKHEDPAQTPPICPFLPNTVGRKAVTVTVVFVTLTFLLGIVLATTTNLFGKT *F LNQTNDPKIHVDGKLVVVSIKGWGGMPTRGNLKPFKLPVSKVIISETPPEICTTQDSCSYWTRVTQSRHMDTFNWSQVGY *F NFLVGGDGRIYEGRGWNYMGDHTRDNNNNSIGITFLGTFRRQEPTPKSLEACQLLIAQGVRLKKLKPDYQLLGHRQITGT *F LMPGEELYRIIQTWNNWYNLTKTWPDLHMTQ *F >PGRP-LC transcript, splice form a (corresponds to CG4432-RB) *F MPFSNETEMSQCSNAKRRVNDPLTGPKNCSTSSTDSGVILNDNVAAFRPEKETKDRGTGEGQFQSKSEEKTESKRISVEH *F TVNITTENVGKTSSPAVSIRSTTISVVSIDDNAIDSSSIDSDSEAEAEDYTVQKLGHQVTYPPNSSHLRDLNQGLTVISR *F HVAPGEAAVPPPNPLEAGIVAKQILNGNLAVATPTSPAGGATQGIGSIALTNSTDVTFGDKHFYEGPVTIQQFLIDNRDK *F WKPGEGPAGGQDNPAFNGGPSTNGSAPGSKHEDPAQTPPICPFLPNTVGRKAVTVTVVFVTLTFLLGIVLATTTNLFGKT *F LNQSKIRDDDDYRQNIPINSTIDLDNIGGGLILRFVERQQWLAQPPQKEIPDLELPVGLVIALPTNSENCSTQAICVLRV *F RLLQTYDIESSQKCDIAYNFLIGGDGNVYVGRGWNKMGAHMNNINYDSQSLSFAYIGSFKTIQPSAKQLSVTRLLLERGV *F KLGKIAPSYRFTASSKLMPSVTDFKADALYASFANWTHWS *F Comments: We have sequenced cDNA corresponding to the PGRP-LC gene (CG4432) *F transcripts. We find three major splice forms: *F LCx (first described by Choe et al. 2002, accession AF500096) *F LCy (Werner et al. 2003, JBC in press, accession numbers not yet assigned) *F LCa (first described by Werner et al. 2000, accession AF207539). This *F transcript corresponds to CG4432-RB. We believe that the CG4432-RA isoform *F does not exist. # *U FBrf0159841 *a Robertson *b H. *t 2003.6.23 *T personal communication to FlyBase *u FlyBase error report for CG9700 on Mon Jun 23 21:19:24 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 23 Jun 2003 21:19:25 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG9700 on Mon Jun 23 21:19:24 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG9700 *F Release: 3 *F Gene annotation error *F Gene CG9700 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Or85e *F ATGGCCAGTCTTCAGTTCCACGGCAACGTCGATGCGGACATCAGGTATGATATTAGCCTGGATCCGGCTAGGGAATCGAA *F TCTCTTCCGTCTGCTAATGGGACTCCAGTTGGCGAATGGCACGAAGCCATCGCCGCGGTTACCCAAATGGTGGCCAAAGC *F GGCTGGAAATGATTGGTAAAGTGCTGCCCAAAGCCTATTGTTCCATGGTGATTTTCACCTCCCTGCATTTGGGTGTCCTG *F TTCACGAAAACCACACTGGATGTCCTGCCGACGGGGGAGCTGCAGGCCATAACGGATGCCCTCACCATGACCATAATATA *F CTTTTTCACGGGCTACGGCACCATCTACTGGTGCCTGCGCTCCCGGCGCCTCTTGGCCTACATGGAGCACATGAACCGGG *F AGTATCGCCATCATTCGCTGGCCGGGGTGACCTTTGTGAGTAGCCATGCGGCCTTTAGGATGTCCAGAAACTTCACGGTG *F GTGTGGATAATGTCCTGCCTGCTGGGCGTGATTTCCTGGGGCGTTTCGCCACTGATGCTGGGCATCCGGATGCTGCCGCT *F CCAATGTTGGTATCCCTTCGACGCCCTGGGTCCCGGCACATATACGGCGGTCTATGCTACACAACTTTTCGGTCAGATCA *F TGGTGGGCATGACCTTTGGATTCGGGGGATCACTGTTTGTCACCCTGAGCCTGCTACTCCTGGGACAATTCGATGTGCTC *F TACTGCAGCCTGAAGAACCTGGATGCCCATACCAAGTTGCTGGGCGGGGAGTCTGTAAATGGCCTGAGgttggtatctgt *F tccacagaaattcaattcaaaaactttccgttcttgcaacttattttgttttcgtgttacaactttaaatcaattcaact *F tttattctttgttttttatattttaagttttaattcgttaatactaatgactttttattttagTTCGCTGCAAGAGGAGT *F TGCTGCTGGGGGACTCGAAGAGGGAATTAAATCAGTACGTTTTGCTCCAGGAGCATCCGACGGATCTGCTGAGATTGTCG *F GCAGGACGAAAATGTCCTGACCAAGGAAATGCGTTTCACAACGCCTTGGTGGAATGCATTCGCTTGCATCGCTTCATTCT *F GCACTGCTCACAGGAGTTGGAGAATCTATTCAGTCCATATTGTCTGGTCAAGTCACTGCAGATCACCTTTCAGCTTTGCC *F TGCTGGTCTTTGTGGGCGTTTCGGGTACTCGAGAGGTCCTGCGGATTGTCAACCAGCTACAGTACTTGGGACTGACCATC *F TTCGAGCTCCTAATGTTCACCTATTGTGGCGAACTCCTCAGTCGGCATAGTATTCGATCTGGCGACGCCTTTTGGAGGGG *F TGCGTGGTGGAAGCACGCCCAT *F Protein sequence: *F >Or85e *F MASLQFHGNVDADIRYDISLDPARESNLFRLLMGLQLANGTKPSPRLPKWWPKRLEMIGKVLPKAYCSMVIFTSLHLGVL *F FTKTTLDVLPTGELQAITDALTMTIIYFFTGYGTIYWCLRSRRLLAYMEHMNREYRHHSLAGVTFVSSHAAFRMSRNFTV *F VWIMSCLLGVISWGVSPLMLGIRMLPLQCWYPFDALGPGTYTAVYATQLFGQIMVGMTFGFGGSLFVTLSLLLLGQFDVL *F YCSLKNLDAHTKLLGGESVNGLSSLQEELLLGDSKRELNQYVLLQEHPTDLLRLSAGRKCPDQGNAFHNALVECIRLHRF *F ILHCSQELENLFSPYCLVKSLQITFQLCLLVFVGVSGTREVLRIVNQLQYLGLTIFELLMFTYCGELLSRHSIRSGDAFW *F RGAWWKHAH *F Comments: The version of this gene in the sequenced D. mel strain is a *F pseudogene resulting from deletion of the 3' end. Only the above portions *F correspond to the intact gene, as shown by Leslie Vosshall's cDNA in GenBank *F for DOR104, and by the D. pseuoobscura comparison. What is missing is the end *F of exon 2, intron 2 (present in Dp, but of course I can't be completely *F confident about Dm until the intact gene is sequenced from other strains), and *F all of exon 3. This gene *F >is intact in Dp. # *U FBrf0159842 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for CG32693 on Tue Jun 24 18:20:52 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:20:52 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG32693 on Tue Jun 24 18:20:52 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG32693 *F Release: 3 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG32693. *F Comments: As a result of working up the Gr family in Dp I have come to *F recognize this gene as a member of the Gr family. I propose the name Gr9a for *F it following our convention of naming these genes for their cytological *F location. Problematic for Dp I know, but consistent with the rest of the Gr *F family in Dm, and that's where they are going to be studied, so it's *F appropriate. # *U FBrf0159861 *a Shih *b H.P. *t 2003.6.25 *T personal communication to FlyBase *u *F Date: Wed, 25 Jun 2003 12:11:16 \-0700 *F To: Gillian Millburn (Genetics) *F From: Hsin-Pei Shih *F Subject: Re: FlyBase query *F Cc: Mark Peifer jpringle@imap.unc.edu *F Dear Gillian, *F Sorry for the confusion. When we wrote the paper, we claimed sip2 (66B) as *F the DmUba2, so renamed .. CG9188/27C .. into sip2 (in JCS). The reason is *F that the 'old' sip2 (66B) now has a real name as DmUba2 (66B), so we *F renamed .. CG9188/27C .. to 'new' sip2 (CG9188/27C). *F Therefore, the right info should be following as *F DmUba2 (66B; old sip2 data should be corrected into DmUba2) *F sip2 (CG9188/27C) should be stated as in JCS paper *F I hope it will help you to clarify this confusion *F Thank you very much! *F Hsin *F At 05:25 PM 6/25/2003 \+0100, you wrote: *F >Dear Dr. Shih, *F > *F >I am curating your paper for FlyBase: *F > *F >Shih et al., 2002, J. Cell Sci. 115(6): 1259--1271 *F > *F >and I am hoping you can help clear up some confusion about the 'sip2' *F >gene. *F > *F >In the paper you say that sip2 corresponds to CG9188. *F > *F >CG9188 maps to 27C. *F > *F >However, we already have a record of a 'sip2' (septin interacting *F >protein) gene in FlyBase from your abstract: *F > *F >Shih et al., 1998, A. Dros. Res. Conf. 39: 371C *F >Novel putative protein partners of Drosophila septins identified in a *F >two-hybrid screen. *F > *F >and also from a personal communication to FlyBase: *F > *F >Shih, 1998.3.17, personal communication to FlyBase (FBrf0102083). *F > *F >The cytology you gave for sip2 in the personal communication was '66B5 *F >or/and 66B11' which does not agree with the cytology for CG9188. *F > *F >My questions are: *F > *F >1. is the 'sip2' gene mentioned in your 2002 J. Cell Sci. paper the *F >same 'sip2' gene as the one mentioned in the Drosophila Conference *F >abstract and the personal communication ? *F > *F >2. if it is the same gene, which information is correct *F >i.e. does CG9188 correspond to sip2 (in which case the cytology in the *F >personal communication was in error), or is CG9188 a typographical *F >error (in which case which CG does sip2 correspond to), *F > *F >any help you can give in clearing this up so that I can get the *F >information correct in FlyBase would be much appreciated, *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- # *U FBrf0159866 *a Andrade *b R. *c M. *d Deal-Herr *e K. *f Cook *t 2003.5.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed May 21 01:21:08 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(3R)BSC34 *F Isolation and Characterization of Df(3R)BSC34 *F Rachel Andrade, Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC34 was isolated as a P transposase-induced male recombination *F event involving P{lacW}howj5B5 and P{PZ}klgrN712. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{lacW}howj5B5/P{PZ}klgrN712 ca1 males. Polytene chromosome squashes *F showed the breakpoints 93F13-94A2;94D1-2. Df(3L)BSC34 failed to complement *F P{lacW}Dph5L4910. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159867 *a Bloomington Drosophila Stock Center *b ?. *t 2003.5.22 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Thu May 22 23:53:25 2003 *F Subject: phenotypes of KG03177 & KG03334 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: phenotypes of KG03177 & KG03334 *F The chromosomes that carry P{SUPor-P}CG2201KG03177 and *F P{SUPor-P}NHE2KG03334, although previously reported to be viable *F and fertile, are semi-viable. Fertility of homozygotes has not been *F determined. Whether the semi-lethality is caused by the insertion or *F maps elsewhere is not known. # *U FBrf0159868 *a Dean *b D. *t 2003.6.5 *T personal communication to FlyBase *u *F From dd35@cornell.edu Thu Jun 05 03:15:22 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: noncomplementation data *F Years ago, I had ordered (old stock \#s) P451, a mutation in turtle, *F and P838, a mutation in Tps1, and tested these alleles for *F noncomplementation with alleles generated by Szidonya and Reuter, *F 1988 Cytogenetic analysis of the echinoid (ed), dumpy (dp) and clot *F (cl) region in Drosophila melanogaster. Genet. Res. 51(3): 197--208 *F FBrf0048137. I never followed up on the project, but in case *F someone else might find it useful, please add to the records for the *F corresponding genes that P451 does not complement jf4 alleles I was *F sent, and P838 does not complement any jf5 alleles I was sent. If *F people who work on turtle or Tps1 want more alleles, they can write *F to the Szeged stock center. *F Thanks, *F DD *F \-- *F 'I look through a microscope at some Golgi apparatus. *F Golgi, oh, woe is me!' *F \-Phish *F Derek Dean *F 607-275-0745 (home) *F 607-254-4358 (work) *F Booker Lab *F 119 Mudd *F Cornell University *F Ithaca, NY 14853 USA *F dd35@cornell.edu # *U FBrf0159869 *a Voas *b M. *t 2003.6.5 *T personal communication to FlyBase *u *F From voas@wi.mit.edu Thu Jun 05 03:09:47 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: rhinoceros *F I'm a researcher in the lab of Ilaria Rebay and I'm writing to correct *F some mistakes that have been made in the record for the gene rhinoceros *F (rno). The record was begun based upon an abstract by our lab for the *F 1999 DRC. We later found that the this gene corresponds to split ends *F (spen) and our lab's data was published using the name spen. Since then *F we decided to reuse the name rhinoceros (rno) to refer to gene CG7036 and *F information from my 2002 DRC abstract has been added to the web page for *F rno. If possible, I would like to continue using the name rno for CG7036 *F and have the information that refers to the Rebay 1999 abstract removed *F from the rno page. *F One last thing. The page for CG7036 cites data from my 2001 DRC abstract, *F including defects in the dorsal appendages in mutant egg chambers. I have *F since found that this result is an artefact. If possible, please remove *F this data. *F Thank you for your help. *F Matt Voas # *U FBrf0159870 *a Lengyel *b J. *t 2003.6.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Jun 04 15:49:28 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Mae-UAS.6.11}bowlRBG *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Judith Lengyel, UCLA (5/03). *F P{Mae-UAS.6.11}bowlRBG was generated by a precise P transposase-induced *F replacement of the P{lacW} element in P{lacW}bowlk08617 with P{Mae-UAS.6.11}. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159871 *a Bejsovec *b A. *t 2003.6.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 03 17:48:47 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: ElfLR16 and eRF1KY7 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Amy Bejsovec, Duke U. (5/03). *F ElfLR16 (synonym eRF3LR16) and eRF1KY7 are EMS mutations isolated as *F nonsense suppressors of the wgPE4 mutation. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159872 *a Cook *b K. *t 2003.6.3 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 03 14:42:43 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Cytological Breakpoints of Df(2L)ade3 *F The Stock Center received a stock of Df(2L)ade3 from Betsy Wilder, U. of *F Pennsylvania, which her lab used in Cohen et al., 2002, Dev. Cell 2(4): *F 437-448 (FBrf0146974). She said the deletion originated with the work of *F Tiong and Nash (Genetics 1990 124:889--897; FBrf0051986). My polytene *F squashes showed that the deletion has breakpoints 27D1-2;27F1-2. The *F deletion breaks within both doublets to form a thick fusion band. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159873 *a Andrade *b R. *c K. *d Cook *t 2003.6.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jun 02 16:46:50 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(3L)BSC35 *F Isolation and characterization of Df(3L)BSC35 *F Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC35 was isolated as a P transposase-induced male recombination *F event involving P{EP}smgEP3556 and P{lacW}l(3)j2B9j2B9. The deletion *F was isolated as a rhove-1-e1 recombinant chromosome from the cross *F rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{EP}smgEP3556/P{lacW}l(3)j2B9j2B9 e1 males. The mini-white marker *F from at least one insertion was retained on the deletion *F chromosome. Polytene chromosome squashes showed the breakpoints *F 66F1-2;67B2-3 (the distal breakpoint fell within the 66F1,2 doublet band, *F leaving a portion of distal 66F1,2 undeleted). Df(3L)BSC35 failed to *F complement Rdl1, P{PZ}bol1 and P{lacW}aayS042314. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159874 *a Davis *b R. *t 2003.5.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 27 21:20:58 2003 *F To: rd120@gen.cam.ac.uk *F Subject: P{tubP-GAL80ts} construct and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Ron Davis, Baylor College of Medicine (5/03). *F P{tubP-GAL80ts} carries sequences encoding a temperature-sensitive GAL80 *F expressed under the control of the alphaTub84B promoter. The construct was *F described in McGuire et al. 'Temporal and Regional Gene Expression *F Targeting with the Conventional GAL4/UAS System in Drosophila', A. Dros. *F Res. Conf. 44 2003 :153 (FBrf0154707). *F Homozygous viable and fertile insertions include P{tubP-GAL80ts}9 on the *F X chromosome, P{tubP-GAL80ts}20 on the second chromosome, and *F P{tubP-GAL80ts}2 and P{tubP-GAL80ts}7 on the third chromosome. *F P{tubP-GAL80ts}10 is inserted on the second chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159875 *a Lengyel *b J. *t 2003.5.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 27 19:03:55 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-drm.G} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Judith Lengyel, UCLA (5/03). *F P{UAS-drm.G}6.1 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-drm.G}2.1 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159876 *a Dearolf *b C. *t 2003.5.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 27 15:29:07 2003 *F To: rd120@gen.cam.ac.uk *F Subject: P{Cg-GAL4.A}2 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Chuck Dearolf, Massachusetts General Hospital (5/03). *F P{Cg-GAL4.A}2 is a homozygous viable and fertile, second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159877 *a Bloomington Drosophila Stock Center *b ?. *t 2003.5.27 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue May 27 15:25:22 2003 *F To: rd120@gen.cam.ac.uk, cy200@gen.cam.ac.uk, gm119@gen.cam.ac.uk *F Subject: Morin et al. insertions *F Gillian, Chihiro, Rachel-- *F Can I persuade one of you to curate the transposon insertions from Table 1 *F of Morin et al. 2001 (FBrf0141725)? I've attached a copy of the paper. *F There aren't transposon entries for the three constructs on the public view *F of FlyBase, but I'm pretty sure that you have dealt with them already and *F named them P{PTT-GA}, P{PTT-GB} and P{PTT-GC}. I have stocks for *F P{PTT-GA}III-1b, P{PTT-GB}III-3b and P{PTT-GC}III-4b. *F Muchos gracias! *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159878 *a Bloomington Drosophila Stock Center *b ?. *t 2003.5.27 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Tue May 27 01:25:02 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Miscellaneous Insertions *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: Miscellaneous Insertions *F Background: These insertions are in FlyBase without an associated *F reference. The information we received from the donor of the stock, or have *F inferred from the genotype of the stock, is provided here. *F Donor: Michael Bender, University of Georgia (1998) *F P{hs-EcR.A}60.19 *F This is a viable and fertile insertion on chromosome 2 of a construct *F described in FBrf0128551. *F P{hs-EcR.B2}1.1 *F This is a viable and fertile insertion on chromosome 2 of a construct *F described in FBrf0128551. *F P{hs-EcR.B1}MB1 *F This is a viable and fertile insertion on chromosome 3 of a construct *F described in FBrf0128551. *F Donor: Ruth Lehmann, Skirball Institute, NYU (1998) *F P{GAL4::VP16-nos.UTR}MVD1 *F This is a viable and fertile insertion on chromosome 3 of a construct *F described in FBrf0100715. *F Donor: Mark Peifer, University of North Carolina (1998) *F P{UAS-arm.S2}A1 *F This is a viable and fertile insertion on chromosome 1 of a construct *F described in FBrf0093638. *F P{UAS-arm.S10}C *F This is a viable and fertile insertion on chromosome 3 of a construct *F described in FBrf0093638. *F Donor: Norbert Perrimon, Harvard Medical School (1995) *F P{GAL4-Hsp70.sev}2 *F This is an insertion on chromosome 2 of a construct described in FBrf0080361. *F P{UAS-Hraf.gof}ra2 *F This is a viable and fertile insertion on chromosome 1 of a construct *F described in FBrf0076497. *F P{UAS-phl.gof}F179 *F This is a viable and fertile insertion on chromosome 3 of a construct *F described in FBrf0076497. *F P{GAL4-Hsp70.PB}2 *F This is a lethal insertion on chromosome 2 of a construct described in *F FBrf0076602. *F P{GAL4-Hsp70.PB}31-1 *F This is an insertion on chromosome 3 of a construct described in FBrf0076602. *F Donor: Peter Wensink, Brandeis University, via Rod Nagoshi, University of *F Iowa (1998) *F P{T&agr;2-lacZ.1}147 (= P{Talpha2-lacZ.1}147) is a viable and fertile *F insertion on chromosome 2 of a construct described in FBrf0049559. # *U FBrf0159879 *a Edgar *b B. *t 1998.9.14 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Mon May 26 23:57:54 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Assorted Insertions *F Personal communication from: Bruce Edgar, Fred Hutchinson Cancer Research *F Center *F To: Bloomington Drosophila Stock Center *F Subject: Assorted Insertions *F Dated: 14 September 1998 and 23 February 1999 *F Background: These insertions are in FlyBase without an associated *F reference. The information we received from the donor of the stock, or have *F inferred from the genotype of the stock, is provided here. *F P{UAS-CycE.L}ML1 *F This is a homozygous viable and fertile insertion on chromosome 3. *F P{UAS-Dp.D}1-4b *F This is a homozygous viable and fertile insertion on chromosome 3. *F P{UAS-Dp.D}8C *F This is an insertion on chromosome 2. *F P{UAS-E2f.N}3B *F This is a homozygous viable and fertile insertion on chromosome 3. *F P{UAS-E2f.N}5A *F This is an insertion on chromosome 2. *F P{UAS-p35.H}BH1 *F This is a homozygous viable and fertile insertion on chromosome 2. *F P{UAS-p35.H}BH2 *F This is a homozygous viable and fertile insertion on chromosome 3. *F P{UAS-stg.N}4 *F This is a homozygous viable and fertile insertion on chromosome 3. *F P{UAS-stg.N}16 *F This is a homozygous viable and fertile insertion on chromosome 2. # *U FBrf0159880 *a Montell *b D. *t 1998.11.25 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Mon May 26 23:21:57 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Insertions of RhoL and Cdc42 constructs *F Personal communication from: Denise Montell, Johns Hopkins School of Medicine *F To: Bloomington Drosophila Stock Center *F Subject: Insertions of RhoL and Cdc42 constructs *F Dated: 25 November 1998 *F Background: These insertions are in FlyBase without an associated *F reference. The information we received from the donor of the stock, or have *F inferred from the genotype of the stock, is provided here. *F Information communicated: *F P{UAS-Cdc42.V12}LL1 *F a homozygous viable and fertile insertion on chromosome 2 *F P{hs-RhoL.N25}AM1 *F the location of this insertion is unknown *F P{hs-RhoL.V20}AM1 *F an insertion on chromosome 2; the insertion chromosome is homozygous lethal *F P{UAS-RhoL.N25}AM1 *F an insertion on chromosome 2; the insertion chromosome is homozygous lethal *F P{UAS-RhoL.V20}AM1 *F a homozygous viable and fertile insertion on chromosome 3 # *U FBrf0159881 *a Davis *b T. *t 2003.5.6 *T personal communication to FlyBase *u *F From wpttd@forest.cf.ac.uk Tue May 06 16:16:55 2003 *F To: rd120@gen.cam.ac.uk *F Subject: elbow gene *F Dear Rachel *F i have been perusing some of the elbow b gene literature and have a few queries. *F The Flybase entry for elB states class i alleles enhance Sco but class ii *F alleles do not yet the alleles *F el4, el5 and el6 are given as class ii but all three enhance Sco? *F . *F . *F . *F thanks *F Terry *F Dr Terence Davis *F Pathology *F UWCM *F Heath Park *F Cardiff CF14 4XN *F Wales, UK *F tel \+44-29-20744964 *F fax \+44-29-20745121 *F \------------------------------------------------------------------------ *F From rd120@gen.cam.ac.uk Wed May 21 16:14:22 2003 *F To: wpttd@cardiff.ac.uk *F Subject: Re: elbow gene *F Hi Terry, *F sorry to take so long to get back to you on this. I've been away and *F then it is quite tricky.... *F The history is that the text in the following paragraph dates back to *F Lindsley and Zimm (page 194-195, where it was associated with the *F 'elbow' entry (i.e. elbow A and B had not been defined at that point). *F Michael apparently wrote this text (page 194-195). *F \------------------------------------------------ *F Wings extended and bent backward, often warped and *F shortened; sometimes blistered and nicked. Alulae *F reduced in size with reduced number of marginal *F bristles \-- may fuse with wing blade. Venation reduced *F by terminal shortening of L5 and of crossveins. *F Halteres reduced, often to stubs. Eye size decreased *F (variable, even with strong alleles). Weak alleles may *F overlap wild type and show only a reduction in number *F of marginal bristles on alulae. Some alleles may be *F semi-lethal when hemizygous. Class (i) alleles enhance *F Sco, are semi-lethal with alleles of l(2)35Ba and show *F a weak noc phenotype when heterozygous with strong noc *F alleles or noc deletions. Class (ii) alleles do not *F interact with these loci. All alleles more extreme *F when hemizygous; strong alleles nearly apterous. *F Part of el-noc complex. *F \------------------------------------------------ *F When elA and elB were made 'genes' in their own right the text was *F simply propagated to both new records. *F Judging from this information we have from your paper *F Davis et al., 1997, Hereditas 126(1): 67--75 ... *F 'The elbow locus is made up of two genes, @elA@ and @elB@, each of *F which has a distinct phenotype when mutant. Mutations in @elA@ have a *F strong phenotype with marked disruptions of the wing. Mutations in *F @elB@ are weak, mainly affecting the alula and wing bristles. @elA@ *F and @elB@ are dominant enhancers of each other.'..... *F the 'Wings extended' paragraph of text needs to be updated (for example *F one update is that l(2)35Ba and Sco are now known to correspond to noc) *F \- I have a plan for this ,whereby this paragraph will be attributed to *F \*x FBrf0056834 == Ashburner, 1992, Lindsley, Zimm, 1992: 194--195 which *F will de-emphasis it and put a long-ago date to it. *F We are also in trouble about the alleles. When such a 'gene record *F split' occurs it is very difficult for us to partition the alleles, as *F we do not generally have enough information to know which of the new *F genes the alleles represent. This is particularly difficult in cases *F as genetically complex as the elbow-noc region. In the following table *F I have enumerated the way it is in FlyBase at the moment \- the old *F symbol in the first column and the alleles in the current version in *F the right hand column. In those cases where there is both an elA and *F an elB allele, each record in FB has a note saying that it might actually be *F an allele of the other. *F Old symbool Current symbol *F el1 elA1 *F el2 elA2, elB2 *F el3 elA3, elB3 *F el4 elB4 *F el5 elB5 *F el6 elB6 *F el7 elB7 *F el8 elA8, elB8 *F el9 elB9 *F el10 elA10, elB10 *F el11 elA11, elB11 *F el12 elA12, elB12 *F el13 elA13, elB13 *F el21 elA21, elB21 *F el22 elA22, elB22 *F el23 elA23, elB23 *F el14 has become Df(2L)el14, hence not in this list. *F I'm sending you this list in case you can cast any light on whether any *F of these 'el' alleles with both an elA and elB allele can be more *F accurately assigned to elA or elB. It is not transparently obvious why *F some alleles have become alleles of elA, some elB and some both, though *F such things are usually based on figures in papers (e.g. el4, el5, *F el6, el7 and el9 became elB alleles because of your figure 2 in *F Davis et al., 1997, Hereditas 126(1): 67--75. *F The classification described by Ashburner in L&Z into class i and class *F ii is extremely odd. *F \---------------------------------------------------------------------- *F Class i alleles *F \--------------- *F The following alleles were marked as class i alleles in L&Z \- el1, *F el2, el3 *F 'Class (i) alleles enhance Sco, are semi-lethal with alleles of l(2)35Ba *F and show a weak noc phenotype when heterozygous with strong noc alleles *F or noc deletions.' *F Relevent FlyBase info from 'el1' i.e. elA1 record: *F \*E FBrf0093413 == Davis et al., 1997, Hereditas 126(1): 67--75 *F \*i el1 *F \*k Phenotype manifest in: wing *F \*k Phenotype manifest in: wing vein L4 *F \*k Phenotype manifest in: wing vein L5 *F \*k Phenotype manifest in: alula *F \*k Phenotype manifest in: dorsal row *F \*k Phenotype manifest in: costal cell *F \*k Phenotype manifest in: wing vein L1 *F \*k Both the anterior and posterior wing are severely reduced in hemizygotes, *F \*k wing vein L4 is shortened, and wing vein L5 and the alulae are absent. *F \*k The submarginal cell (between L2 and L3) appears relatively normal. *F \*k In some wings the costal cell is extended and wing vein L1 reaches *F \*k the wing margin more distally than normal. The triple row is reduced *F \*k in the dorsal rows but not the ventral row. *F \*k @elA1@/@Df(2L)b83d29a@ adults are wild-type. @elA1@/@Df(2L)b84a2@ *F \*k adults have an 'elbow' phenotype. *F Relevent FlyBase info from 'el2' i.e. elA2, elB2 records: *F elA2 has: *F \*E FBrf0093413 == Davis et al., 1997, Hereditas 126(1): 67--75 *F \*i el2 *F \*o ethyl methanesulfonate *F \*k Weak allele. Lethal in double heterozygous combination with @Df(2L)TE35B-1@, *F \*k @Df(2L)TE35BC-8@, @In(2L)TE35B-13@, @Df(2L)el28@, @Df(2L)A400@ and *F \*k @Df(2L)TE35B-7@ (rare @Df(2L)el28@, @Df(2L)A400@ and @Df(2L)TE35B-7@ *F \*k adult escapers are occasionally seen). Semi-lethal in double heterozygous *F \*k combination with @Tp(2;2)A446@, @Df(2L)n78l3@, @Df(2L)fn3@ and *F @Df(2L)TE35B-6@. *F \*S Genetic interaction (effect, anatomy): enhancer | dominant, chaeta { *F nocSco } *F elB2 has nothing relevent *F Relevent FlyBase info from 'el3' i.e. elA3, elB3 records: *F Neither elA3 nor elB3 have anything relevent *F CONCLUSION: Nothing for 'el1' either supports or contradicts the *F Class i definition. Data for 'el2' does not directly contradict 'are *F semi-lethal with alleles of l(2)35Ba' and does support 'Class (i) *F alleles enhance Sco' with respect to the chaeta phenotype. There is no *F data for 'el3'. *F \---------------------------------------------------------------------- *F Class ii alleles *F \---------------- *F The following alleles were marked as class ii alleles in L&Z \- el4, *F el5, el6 *F 'Class (ii) alleles do not interact with these (Sco, l(2)35Ba, noc) *F loci.' *F Relevent FlyBase info from 'el4' i.e. elB4 record: *F \*E FBrf0093413 == Davis et al., 1997, Hereditas 126(1): 67--75 *F \*k Phenotypic class: visible | recessive *F \*k Phenotype manifest in: wing *F \*k Phenotype manifest in: alula *F \*k Hemizygotes have reduced wings, and, especially, reduced alulae. *F \*S Genetic interaction (effect, class): enhancer | dominant { nocSco } *F Relevent FlyBase info from 'el5' i.e. elB5 record: *F \*E FBrf0093413 == Davis et al., 1997, Hereditas 126(1): 67--75 *F \*k Phenotypic class: visible | recessive *F \*k Phenotype manifest in: alula & macrochaeta *F \*k Phenotype manifest in: wing | posterior *F \*k Phenotype manifest in: wing vein L5 *F \*k Hemizygotes are almost wild-type, but have a reduction in the number *F \*k of marginal bristles on the alulae and along the posterior wing margin. *F \*k Wing vein L5 is sometimes shortened. *F \*S Genetic interaction (effect, class): enhancer | dominant { nocSco } *F Relevent FlyBase info from 'el6' i.e. elB6 record: *F \*E FBrf0093413 == Davis et al., 1997, Hereditas 126(1): 67--75 *F \*k Phenotypic class: visible | recessive *F \*k Phenotype manifest in: wing *F \*k Phenotype manifest in: alula *F \*k Hemizygotes have reduced wings, and, especially, reduced alulae. *F \*S Genetic interaction (effect, class): enhancer | dominant { nocSco } *F CONCLUSION: *F Thus your data from FBrf0093413 is directly at odds, in terms of the Sco *F interaction data, with that from L&Z. *F \---------------------------------------------------------------------- *F The best I can do for now is attribute the 'class' info to the *F Ashburner, 1992, Lindsley, Zimm, 1992: 194--195 reference, for the same *F reasons as with the chunk of text from the gene level. It is not *F traceable and therefore we can't dig any further. I'm not sure that *F the 'class' info contributes any useful info and if I deal with it in *F this way then at least the info is not lost, only de-emphasised. *F It may be that this is more than you had bargained for.... any help you *F can give clarifying the records would be gratefully received. I'll *F work on the updates now, but it will be some weeks before any changes *F get made in the main FlyBase dataset. The records will certainly look *F better for the changes you have provoked! They haven't had a spring *F clean for many years. *F All the best, *F Rachel. *F \------------------------------------------------------------------------ *F From wpttd@forest.cf.ac.uk Thu May 22 15:32:02 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Re: elbow gene *F Dear Rachel *F thanks for your detailed reply. *F Unfortunately most of the info concerning the el alleles and their *F interactions with noc/l(2)35Ba are *F buried in Mike's files (yes that lot!!). I used to know most of the relevant *F but it's been some time. The *F rest of the data is buried in the various of Mike's papers in Genetics from *F 1979-1983, (at least for alleles *F el1, el2, el3 and el4). *F But it is clear that el1 is elA (deletes the putative elA transcript CG15283) *F and class ii (i.e. it does not *F enhance Sco or have a noc phene with noc alleles). *F el2 and el3 are class i as both strongly enhance sco, and are semi-lethal over *F l(2)35Ba. In fact I believe *F that el2 is actually an allele of l(2)35Ba/noc!! see my 1997 paper. This is *F based upon its recombination *F frequency which puts it well proximal of elA and its lethality over 1(2)35Ba *F alleles. *F el4, el5, el6, el7 and el9 are class i based upon slight but real enhancement *F of Sco and are marked as elB *F as they break in the first intron of the elB gene. I'm not sure of their *F interaction with l(2)35Ba/noc, but *F elB alleles do interact with noc. *F el24 is more confusing, as it breaks proximal to putative elA gene but has *F strong el phenotype and does not *F enhance sco (putative elA class ii). *F in summary: *F class i alleles are elB alleles *F class ii are elA alleles *F the other alleles in the list are more difficult as their interactions with *F l(2)35Ba/noc are not known to me *F (they are probably in Mike's files), and they have little real effect on Sco. *F they are all very weak so *F difficult to assign and are all point mutations not aberrations. *F Judging by dorfmann et al's paper it may be difficult to assign weak point *F alleles to elB or to l(2)35Ba as *F the two genes clearly interact and have similarities of phenotype *F . *F . *F . *F Best wishes *F Terry *F \------------------------------------------------------------------------ *F From rd120@gen.cam.ac.uk Thu May 22 16:07:12 2003 *F To: wpttd@cardiff.ac.uk *F Subject: Re: elbow gene *F Hi Terry, *F the state of play is that I have renovated the records for elA and elB *F and am about to leave until June 3rd \- so I can't do much more now. At *F least the records are much tidier for the attention, in the meantime. *F I think what you are telling me directly contradicts what is in L&Z for *F several alleles, and therefore I should do a correction, citing your *F mail as the source. The contradictions are *F L&Z Your mail *F el1 Class i Class ii *F el2 Class i Class i *F el3 Class i Class i *F el4 Class ii Class i *F el5 Class ii Class i *F el6 Class ii Class i *F I'm happy to change the class designations, if that is your intention. *F I'll have to keep the old statements in text, but will cross reference *F to the update (your mail). *F You also say *F >class i alleles are elB alleles *F >class ii are elA alleles *F which means I should delete el2 and el3 from the elA record and *F leave them in elB, right? *F I'll write to Shilo (corresponding author for FBrf0151266) now. *F cheers, *F Rachel. *F From wpttd@forest.cf.ac.uk Thu May 22 16:20:36 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Re: elbow gene *F > L&Z Your mail *F > *F >el1 Class i Class ii *F >el2 Class i Class i *F >el3 Class i Class i *F >el4 Class ii Class i *F >el5 Class ii Class i *F >el6 Class ii Class i *F \-------- Basically yes. But as I said the el4, el5, el6 interactions with *F l(2)35Ba are problematical. it *F seems that elB alleles over l(2)35Ba are semi-lethal and/or weak noc, elA1 *F over deletions inc l(2)35Ba are *F actually el you see the problem. i.e alleles. of both these genes seem to *F interact with l(2)35Ba but *F in different ways, this has never been fully investigated. *F Thus I think the definitive interaction is with Sco as this seems to pose no *F contradictions. The real data *F are buried in Mike's files. *F >You also say *F >>class i alleles are elB alleles *F >>class ii are elA alleles *F > *F >which means I should delete el2 and el3 from the elA record and *F >leave them in elB, right? *F \---yes. But it is possible that at least el2 is l(2)35Ba!! *F Terry # *U FBrf0159882 *a Morin *b X. *t 2003.6.19 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Mon Jun 09 11:51:00 2003 *F To: xavier.morin@kcl.ac.uk, william.chia@kcl.ac.uk *F Subject: Helping FlyBase: PTT lines *F Dear Xavier and Bill, *F I'm writing about your PTT paper *F \*x FBrf0141725 == Morin et al., 2001, Proc. Natl. Acad. Sci. USA 98(26): *F 15050--15055 *F This paper will give rise to three transposon records, for P{PTT-GA}, *F P{PTT-GB}, P{PTT-GC} in FlyBase. There will also be insertion records *F for P{PTT-GA}III-1b, P{PTT-GB}III-3a etc from Table 1. *F The thing is that I would also like to make records for the insertion *F lines in Tables 2 and 3, however to do this I need to know which of *F P{PTT-GA}, P{PTT-GB} or P{PTT-GC} is inserted in each case. If I knew *F this I would make an allele of the gene in question, e.g. for G5, if it *F was an insertion of P{PTT-GA}, I would make an allele of Tm2, called *F Tm2G5, with an associated insertion P{PTT-GA}Tm2G5. For those *F lines not associated with a known or predicted gene, the insertion *F would go into FlyBase as a simple insertion, e.g. P{PTT-GA}G50. *F Would you be able to tell me which transposon is inserted in each *F line? We may as well get these lines explicitly instantiated in FlyBase *F now, or else we'll just have to do them piecemeal later on. I've *F included a list of the lines at the bottom, so that it is easy for you *F to put the info in a reply message. *F Looking forward to hearing from you. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From morin@ibdm.univ-mrs.fr Thu Jun 19 17:24:39 2003 *F To: rd120@gen.cam.ac.uk *F Subject: protein trap *F Dear Rachel, *F I attach a table which summarises the info you need. Unfortunately, since *F at some point we pooled some cages, some lines arised from a mixture of *F GA/B/C progenitors: they are labelled with a '?'. three lines have both a *F PTT id and a question mark: these came from pools, but I have had other *F subsequent inserts in the same loci and introns, with clearly identified *F GA-B-C PTT progenitors, so I assume they were derived from the same PTT. *F regards, *F Xavier MORIN, PhD *F Developmental Biology Institute of Marseilles (IBDM) *F Case 907, Campus de Luminy *F 13288 Marseille Cedex 9 *F FRANCE *F tel: 33 (0)4 91 26 97 46 *F fax: 33 (0)4 91 26 97 48 *F morin@ibdm.univ-mrs.fr *F www.ibdm.univ-mrs.fr *F \----------------------- *F Known genes: *F G5 C *F G7 A *F G29 ? *F G44 ? *F G53 A(?) *F G74 A(?) *F G109 C *F G126 B *F G129 A *F G138 A *F G158 B *F G169 ? *F G259 A *F G262 B *F G280 C *F G305 A *F G409 B *F G430 A *F G454 ? *F \----------------------- *F Predicted genes: *F G9 A *F G38 ? *F G88 A *F G89 A *F G93 B *F G112 C *F G119 A *F G147 B *F G180 C *F G189 B  *F G196 B *F G198 A *F G245 ? *F G264 C *F G271 A *F G282 C *F G365 C *F \----------------------- *F No known/predicted gene: *F G50 ? *F G108 C *F G116 A *F G123 ? *F G154 B *F G157 A *F G231 C *F G258 A *F G270 A *F G318 B *F G357bis ? *F G145 B *F G215 B *F G214 C *F G260 B *F G276 B *F G281 B *F G284 B *F G287 B *F G304 B *F G341 ? *F G357 B *F G360 ? *F G361 B *F G370 ? *F G377 B *F G392 C *F G413 A *F G419 C *F G428 C (?) *F \----------------------- # *U FBrf0159883 *a Santamaria *b P. *t 2003.6 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jun 24 19:51:09 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Santamaria and Randsholt mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Pedro Santamaria, Centre de Genetique Moleculaire du *F C.N.R.S, Gif sur Yvette (6/03). *F The mutations Dsor1G79, Dsor1G42, rtgG44, rtgG6, elgG178 and *F elgG11 were isolated in the EMS screen described in Santamaria and *F Randsholt, 1995, Mol. Gen. Genet. 246: 282-290 FBrf0080370. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159884 *a Ring *b B. *c D. *d Garza *t 2003.6.10 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Tue Jun 10 20:14:48 2003 *F Subject: PBac Half-P Constructs *F To: flybase-updates@morgan.harvard.edu *F Cc: brian.ring@pharma.novartis.com *F Personal communication from: Brian Ring and Dan Garza, Florida State *F University, Novartis *F To: Bloomington Drosophila Stock Center *F Subject: PBac Constructs from the Project 'Genome Wide Dispersal of *F Half-P Elements in Drosophila Using PiggyBac' *F Dated: 23 May 2003 *F Background: This is the information needed to curate the two piggyBac *F constructs that are present in the set of PBac insertion lines donated to *F the Bloomington Stock Center by Brian Ring and Dan Garza. The primary *F reference is Brian's dissertation, FBrf0152434 (Chapter 4): *F Ring, Brian C., 'Functional Analysis of the Drosophila Genome Using *F Transposable Elements'. Florida State University. 2001 pgs. 130-199. *F Data on the insertion lines themselves will be submitted separately as a *F computer file. *F Information communicated: *F 1) PBac{5HPw+} *F This is a 5' half-P construct marked with w+mC. The minimal 5' P-element *F cis acting sequences and 8 bp target site duplication derived from P{lacW} *F were cloned into the BglII site of Al Handler's pB{Dmw} construct (Handler *F and Harrell, 1999, Insect Molec. Biol. 8:449--457; FBrf0123037) and *F transformed into flies. Handler created pB{Dmw} by cloning the w+mC *F allele (4.2-kb EcoRI fragment) into the HpaI site of the original piggyBac *F plasmid p3E1.2 (Cary et al., 1989, Transposon mutagenesis of baculoviruses: *F analysis of Trichoplusiani transposon IFP2 insertions within the FP-Locus *F of nuclear polyhedrosis viruses. Virology 161: 8--17). The white mini-gene *F interrupts the piggyBac transposase open reading frame, but otherwise *F leaves the piggyBac element intact, with the respective promoters in *F opposite orientation (per Handler and Harrell, 1999). *F Insertions of this construct used for the screens: *F PBac{5HPw+}X7 \- This homozygous viable and fertile X chromosome insertion *F was the progenitor of the A series of insertions. *F PBac{5HPw+}X10 \- This homozygous viable and fertile X chromosome *F insertion was the progenitor of the B series of insertions. *F 2) PBac{3HPy+} *F This is a 3' half-P construct marked with y+mDint. The minimal 3' *F P-element cis acting sequences and 8 bp target site duplication derived *F from P{lacW} were cloned into the 3' end of the yellow mini-gene (Geyer and *F Corces, 1987, Genes Dev. 1: 996--1004; FBrf0046293) in plasmid yellow-BSX, *F a generous gift from Pam Geyer, to form the intermediate plasmid p3HPy+. *F This DNA was then shuttled into a modified site in p3E1.2 (Cary et al., *F 1989, see above) to form pPBac{3HPy+}, which was transformed into flies. *F The resulting yellow mini-gene and 3' P-element interrupt the piggyBac *F gene and reside in opposite orientation with respect to promoters. *F Because intronic sequences necessary for y+ expression in bristles (all *F types), tarsal claw and arista are not present in y+mDint, y- flies *F transformed with PBac{3HPy+} lack y+ pigmentation in these structures. *F Insertions of this construct used for the screens: *F PBac{3HPy+}X0 and PBac{3HPy+}X3 \- These homozygous viable and fertile X *F chromosome insertion were the progenitors of the C series of insertions. # *U FBrf0159885 *a O'Kane *b C. *t 2003.7.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 02:08:30 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-NLS-NES+-GFP}5A & P{UAS-NLS-NESP12-GFP}2A *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Cahir O'Kane, Cambridge University. *F P{UAS-NLS-NES+-GFP}5A is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-NLS-NESP12-GFP}2A is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159886 *a Andrade *b R. *c J. *d Deal *e K. *f Cook *t 2003.7.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 02:45:43 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(3R)BSC38 *F Isolation and Characterization of Df(3R)BSC38 *F Rachel Andrade, Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC38 was isolated as a P transposase-induced male recombination *F event involving P{EP}EP3681 and P{EP}EP3340EP3340. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{EP}EP3681/P{EP}EP3340EP3340 ca1 males. Polytene chromosome squashes *F showed the breakpoints 85F1-2;86C7-8 (i.e. breaks within both doublet bands *F to form a fusion band). Df(3R)BSC38 failed to complement P{PZ}tws02414, *F P{PZ}jumu06439 and hth5E04. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159887 *a Chang *b H. *t 2003.6 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 18:05:30 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Chang constructs and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Henry Chang, Yale University (6/03). *F 1. P{UAS-EGFP-Clc} insertions *F P{UAS-EGFP-Clc}3 and P{UAS-EGFP-Clc}4 are homozygous viable and fertile, *F second chromosome insertions. *F P{UAS-EGFP-Clc}5 is a third chromosome insertion. *F 2. P{GMR-myr.GFP} insertions *F P{GMR-myr.GFP}2R is a homozygous viable and fertile insertion on 2R distal *F to 42D. *F P{GMR-myr.GFP}2L is an insertion on 2L distal to 40A. *F P{GMR-myr.GFP}3R is a homozygous viable and fertile insertion on 3R distal *F to 82B. *F 3. P{GMR-auxillin.C}2 is a homozygous viable and fertile, second *F chromosome insertion. *F 4. P{GMR-shiK39A} insertions *F P{GMR-shiK39A}3 is a third chromosome insertion. *F P{GMR-shiK39A}2 is a second chromosome insertion. *F 5. P{bossT:Arus\HRP} insertions *F P{bossT:Arus\HRP}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{bossT:Arus\HRP}3 is a homozygous viable and fertile, third chromosome *F insertion. *F 6. P{UAS-myr-mRFP} construct *F In this construct, monomeric RFP with a myristoylation signal is expressed *F under the control of UAS. The myristoylation signal is the first 90 amino *F acids of Src64B. *F 7. P{UAS-myr-mRFP} insertions *F P{UAS-myr-mRFP}1 is a second chromosome insertion. *F P{UAS-myr-mRFP}2 is a third chromosome insertion. *F 8. P{GMR-myr-mRFP} construct *F In this construct, monomeric RFP with a myristoylation signal is expressed *F under the control of the GMR promoter. The myristoylation signal is the *F first 90 amino acids of Src64B. *F 9. P{GMR-myr-mRFP} insertions *F P{GMR-myr-mRFP}3 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-myr-mRFP}7 is a homozygous viable and fertile insertion on 3L *F P{GMR-myr-mRFP}2L is an insertion on 2L distal to 40A. *F P{GMR-myr-mRFP}2R is a homozygous viable and fertile insertion on 2R distal *F to 42D. *F P{GMR-myr-mRFP}3R is an insertion on 3R distal to 82B. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159888 *a Santamaria *b P. *t 2003.7.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 18:36:43 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{lacW}l(1)8DaH128B *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Pedro Santamaria, Centre de Genetique Moleculaire du *F C.N.R.S, Gif sur Yvette (6/03). *F P{lacW}l(1)8DaH128B is a lethal P{lacW} insertion that fails to *F complement Df(1)10-70d and is rescued by Dp(1;Y)FF1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159889 *a Lengyel *b J. *t 2003.7.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 19:13:44 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Lengyel constructs and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Judith Lengyel, University of California at Los Angeles (6/03). *F P{UAS-dve.5.0}3 is a homozygous viable and fertile, third chromosome insertion. *F P{UAS-rib.B}HVII is a homozygous viable and fertile, second chromosome *F insertion. *F P{drm-GAL4.7.1}1.1 is a third chromosome insertion. *F P{croc-lacZ.7}3 is a third chromosome insertion. *F P{fkh-GAL4.H}3 is a homozygous viable and fertile, third chromosome insertion. *F P{UAS-sob.G} was constructed by Ryan Green. It carries a wild type sob *F sequence under the control of UAS. *F P{UAS-sob.G}15.1 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159890 *a Brand *b A. *t 2003.7.4 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 19:46:56 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Brand insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Andrea Brand, University of Cambridge (5/03). *F P{UAS-ECFP-beta-actin}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-IR.GFP-FK506-bp2}1 and P{Ubi-tacc.GFP}1 are homozygous/hemizygous *F viable and fertile, X chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159891 *a Ferro *b W. *t 2002.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 20:53:22 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: 99DE complementation groups *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Wouter Ferro, Leiden University Medical Center (12/02). *F A chromosome with the markers st1 in1 kniri-1 pp was mutagenized *F with EMS and screened for recessive lethal and MMS-sensitive mutations that *F fail to complement the chromosome Df(3R)X3F, P{RP49}A3-84F. Ten lethal *F loci and one MMS-sensitive locus were defined. *F Locus Number of alleles Including alleles *F l(3)99DEa 3 l(3)99DEa7-6 *F l(3)99DEb 4 l(3)99DEb3-2 *F l(3)99DEc 1 l(3)99DEc3-13 *F l(3)99DEd 7 l(3)99DEd3-18, l(3)99DEd6-8 *F l(3)99DEe 3 l(3)99DEe6-4 *F l(3)99DEf 2 l(3)99DEf5-1 *F l(3)99DEg 1 l(3)99DEg7-14 *F l(3)99DEh 1 l(3)99DEh7-20 *F l(3)99DEi 1 l(3)99DEi9-27 *F l(3)99DEj 1 l(3)99DEj10-28 *F mus313 1 mus3136-12 *F Note that l(3)99DEh7-20 was semilethal in combination with *F l(3)99DEb3-2. Unfortunately, the l(3)99DEg7-14 line was lost. *F In addition, one allele of warts, wts3-17, was isolated in this screen. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159892 *a Ferro *b W. *t 2002.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 22:09:54 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: 23CD mutation II (please ignore 1st version I sent) *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Wouter Ferro, Leiden University Medical College (12/02). *F An EMS screen for lethal mutations that fail to complement Df(2L)JS17 was *F described in Eeken et al., 1997 (Isolation and characterization of two *F MMS-sensitive mutants in region 23C-23D. A. Dros. Res. Conf. 38: 272A; *F FBrf0092383) *F and in the FlyBase personal communication FBrf0125088. *F cn1 bw1 chromosomes were mutagenized and screened for lethality over *F Df(2L)JS17. The following numbers of alleles were recovered: *F l(2)23Ca 1 *F l(2)23Cb 3 *F l(2)23Cc 4 *F l(2)23Cd 2 *F l(2)23Ce 4 *F l(2)23CDa 4 *F l(2)23CDb 4 *F l(2)23CDc 3 *F l(2)23CDd 4 *F l(2)23CDe 4 *F l(2)23CDf 2 *F l(2)23CDg 3 *F l(2)23Da 5 *F l(2)23Db 1 *F toc 34 *F The following are specific alleles of these complementation groups: *F l(2)23CbA20-2 *F l(2)23CeA14-9 *F l(2)23CeA15-2 *F l(2)23CDaA4-3 *F l(2)23CDaA14-6 *F l(2)23CDbA3-2 *F l(2)23CDcA14-4 *F l(2)23CDdA9-3 *F l(2)23CDdA19-9 *F l(2)23CDfA18-2 *F l(2)23DaA9-4 *F tocA1-7 *F l(2)23Cc is allelic to lilliputian based on the noncomplementation of EMS *F mutations with lillik05431. *F Small deletions isolated in this screen gave the following complementation *F results: *F Df(2L)16 failed to complement mutations in l(2)23CDa and l(2)23CDb *F Df(2L)17 failed to complement mutations in l(2)23CDc and toc *F Df(2L)18 failed to complement mutations in toc, l(2)23CDd and l(2)23CDg *F Df(2L)19 failed to complement mutations in l(2)23CDd and l(2)23CDg *F Df(2L)20 failed to complement mutations in l(2)23CDe and l(2)23CDf *F Df(2L)16 through 20 complemented other mutations from the screen. *F Small deletions from other screens gave the following complementation results: *F Df(2L)JS25 and Df(2L)JS27 failed to complement mutations in l(2)23CDb, *F l(2)23CDc, l(2)23CDd, l(2)23CDe, l(2)23CDf, l(2)23CDg and toc, but *F complemented other mutations from the screen. *F Df(2L)AS2 complemented lilli mutations and failed to complement l(2)23Cd, *F l(2)23Ce and toc mutations, but other mutations from the screen were not *F tested. *F Df(2L)AS4 failed to complement toc, l(2)23CDd, l(2)23CDf and l(2)23Da *F mutations, but other mutations from the screen were not tested. *F Df(2L)AS9 failed to complement toc, l(2)23CDf and l(2)23Da mutations, but *F other mutations from the screen were not tested. *F Df(2L)AS8 failed to complement mutations in l(2)23Cb, l(2)23Ca and okra, *F but complemented other mutations from the screen. *F Df(2L)C28 failed to complement l(2)23Da and l(2)23Db mutations, but *F complemented other mutations from the screen. *F These results allow the loci to be arranged in the distal-to-proximal order: *F l(2)23Cc *F l(2)23Cd and l(2)23Ce *F l(2)23CDa *F l(2)23CDb *F l(2)23CDc *F toc *F l(2)23CDd *F l(2)23CDg *F l(2)23CDe *F l(2)23CDf *F l(2)23Da and l(2)23Db *F l(2)23Cb, l(2)23Ca and okra *F Since these results place l(2)23Ca and l(2)23Cb in 23D, they should be *F renamed l(2)23Dc and l(2)23Dd, respectively. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159893 *a Ferro *b W. *t 2002.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 04 22:24:16 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: l(1)1Bb8-36-2 and dwg11-32 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Wouter Ferro, Leiden University Medical College (12/02). *F l(1)1Bb8-36-2 is a spontaneous mutation rescued by Dp(1;Y)y267g19.1. *F dwg11-32 is a spontaneous mutation rescued by Dp(1;Y)w+y+. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159894 *a Younger *b S. *t 2001.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Sat Jul 05 20:28:42 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Younger P{GawB} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Susan Younger, University of California at San Francisco *F (2/01). *F I. P{GawB}GII5 is homozygous viable and fertile second chromosome *F insertion. In combination with a P{UAS-lacZ} insertion*, staining is seen *F in embryonic peritracheal cells and pericardial cells. *F (*Note: The specific identities of the P{UAS-lacZ} and P{UAS-Notch} *F constructs were not provided.) *F II. P{GawB}G11-1 is a homozygous and hemizygous viable and fertile X *F chromosome insertion. In combination with a P{UAS-lacZ} insertion, *F staining is seen in a subset of the embryonic peripheral nervous system and *F uniformly in stage 9 follicle cells. *F III. P{GawB}112A is a homozygous and hemizygous viable and fertile X *F chromosome insertion generated in the lab of Y.N. Jan. In combination with *F a P{UAS-lacZ} insertion, staining is seen in the embryonic epidermis and in *F the stalk cells and in follicle cells at the anterior and posterior poles *F of the egg chamber. *F IV. P{GawB}109C1 is a homozygous and hemizygous viable and fertile X *F chromosome insertion generated in the lab of Y.N. Jan. In combination with *F a P{UAS-lacZ} insertion, staining is seen in the embryonic epidermis and in *F the follicle cells at the anterior and posterior poles of the egg chamber. *F V. The following insertions were generated by mobilization of P{GawB}109C1 *F in the lab of Y.N. Jan. *F A. P{GawB}109-28 *F 1. A homozygous viable and fertile second chromosome insertion. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in the *F follicle cells of the dorsal appendage. *F B. P{GawB}109-30 *F 1. This insertion is carried on the second chromosome. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in the *F embryonic dorsal midline and peripheral nervous system, the larval ventral *F nerve cord and segmentally in the nervous system, and in stalk cells, *F follicle cells at the poles of the egg chamber and the precursors to the *F follicle cells in the germarium. *F 3. In combination with P{UAS-N}, it is larval lethal. *F 4. In combination with P{UAS-Cdc42.L}, it is larval lethal. *F C. P{GawB}109-39 *F 1. This insertion is carried on the third chromosome. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in *F blastoderm stripes, and in stage 2 follicle cells. *F 1. In combination with P{UAS-N}, it is larval lethal. *F 2. In combination with P{UAS-Cdc42.L}, it is larval lethal. *F 3. In combination with P{UAS-Rac1.L}, it is larval lethal. *F D. P{GawB}109-53 *F 1. This insertion is carried on the third chromosome. *F 2. In combination with a P{UAS-lacZ} insertion, general staining is seen in *F embryos. In ovaries, staining is seen in stalk cells and terminal filament *F cells. *F 1. In combination with P{UAS-numb.W}, it is embryonic-larval lethal. *F 2. In combination with P{UAS-N}, it is larval lethal. *F 3. In combination with P{UAS-Cdc42.L}, it is larval lethal. *F E. P{GawB}sca109-68 (FBti0004036) *F 1. A homozygous lethal, second chromosome insertion. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in *F proneural cells of the wing imaginal disk and in stage 6 follicle cells. *F 3. In combination with P{UAS-numb.W}, it causes bristle defects. *F 4. In combination with P{UAS-N}, it causes bristle defects. *F 5. In combination with P{UAS-Cdc42.L}, it is larval lethal. *F 6. In combination with P{UAS-Rac1.L},it causes bristle defects. *F F. P{GawB}109-69 *F 1. This insertion is carried on the second chromosome. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in *F embryonic dorsal epidermis and in parts of the peripheral nervous system, *F in the larval wing imaginal disk and parts of the brain, and in stage 8 *F follicle cells. *F 3. In combination with P{UAS-Cdc42.L}, it is larval lethal. *F 4. In combination with P{UAS-Rac1.L}, it causes rough eyes. *F G. P{GawB}109-79 *F 1. A homozygous viable and fertile second chromosome insertion. *F 2. In combination with a P{UAS-lacZ} insertion, staining is not seen in *F embryos, but strong staining is seen in stage 2-3 follicle cells. *F 5. In combination with P{UAS-numb.W}, it is embryonic-larval lethal. *F 6. In combination with P{UAS-N}, it is larval lethal. *F 7. In combination with P{UAS-Cdc42.L}, it is larval lethal. *F 8. In combination with P{UAS-Rac1.L},it is embryonic-larval lethal. *F VI. The following insertions were generated in the lab of Gerhard Technau. *F A. P{GawB}1158x *F 1. A homozygous and hemizygous viable and fertile X chromosome insertion. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in *F embryonic ventral muscles and a subset of the CNS. *F B. P{GawB}60IIA *F 1. A homozygous viable and fertile second chromosome insertion. *F 2. In combination with a P{UAS-lacZ} insertion, staining is seen in the *F nervous system. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159895 *a Bender *b M. *t 2003.7.2 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Mon Jul 07 22:09:01 2003 *F Subject: P{hs-EcR.A}60.19 and P{hs-EcR.B2}1.1 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Michael Bender, University of Georgia *F To: Bloomington Drosophila Stock Center *F Subject: P{hs-EcR.A}60.19 and P{hs-EcR.B2}1.1 *F Dated: 2 Jul 2003 *F Background: These two insertions were incorrectly shown as insertions on *F chromosome 2 in the Bloomington stock list from 11/25/1998 through 7/1/2003. *F Both insertions are viable and fertile. *F Information communicated: *F P{hs-EcR.A}60.19 and P{hs-EcR.B2}1.1 are insertions on chromosome 3. # *U FBrf0159896 *a Fleming *b R. *t 2003.7 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Jul 10 16:46:09 2003 *F To: rd120@gen.cam.ac.uk *F Subject: P{Ser-GAL4.GF}1 and P{Ser-GAL4.GF}2 *F The following information was provided to the Bloomington Stock Center by *F Robert Fleming, Trinity College (7/03). *F The regulatory sequences in P{Ser-GAL4.GF} that drive the expression *F pattern come from the 3' region of Serrate and are there in conjunction *F with the Serrate promoter to drive GAL4. There are two independently *F derived inserts of this construct on the second chromosome that were *F brought together by recombination. The P{Ser-GAL4.GF}1 and P{Ser-GAL4.GF}2 *F insertions are homozygous viable and fertile. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159897 *a Park *b J. *t 2003.1 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 11 16:49:38 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Pdf-GAL4.P2.4} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jae Park, University of Tennessee (1/03). *F P{Pdf-GAL4.P2.4}X is a homozygous/hemizygous viable and fertile, X *F chromosome insertion. *F P{Pdf-GAL4.P2.4}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159898 *a Deal *b J. *c K. *d Cook *t 2003.7.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 11 17:09:08 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC36 *F Isolation and characterization of Df(2L)BSC36 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC36 was isolated as a P transposase-induced male recombination *F event involving P{PZ}Nup15401501 and P{GT1}BG00672. The deletion was *F isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 *F cn1 bw1 females x P{PZ}Nup15401501 cn1/dpov1 P{GT1}BG00672; TMS, *F P{Delta2-3}99B males. The mini-white marker from P{GT1}BG00672 was deleted *F or disrupted. Polytene chromosome squashes showed the breakpoints *F 32D1;32D4-E1. Df(2L)BSC36 failed to complement l(2)0400804008. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159899 *a Deal *b J. *c K. *d Cook *t 2003.7.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Jul 11 17:12:36 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC32 *F Isolation and characterization of Df(2L)BSC32 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC32 was isolated as a P transposase-induced male recombination *F event involving P{PZ}dbf1 and P{lacW}l(2)k05812k05812. The deletion was *F isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 *F cn1 bw1 females x P{PZ}dbf1 cn1/dpov1 P{lacW}l(2)k05812k05812; *F TMS, P{Delta2-3}99B males. The miniwhite marker from *F P{lacW}l(2)k05812k05812 was retained. Polytene chromosome squashes *F showed the breakpoints 32A1-2;32C5-D1. Df(2L)BSC32 failed to complement *F UbcD2k13206 and l(2)k03107k03107. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0159902 *a Day *b J.P. *c S.A. *d Davies *t 2003.6.10 *T personal communication to FlyBase *u Cyclic nucleotide Phosphodiesterases. *F Dear Flybase *F Personal communication to Flybase: Cyclic nucleotide Phosphodiesterases *F Day, Jonathan Peter and Davies, Shireen-A. *F We would like to propose a nomenclature for the genes encoding cyclic *F nucleotide-specific phosphodiesterases (PDEs) in Drosophila *F melanogaster. *F Most of these remain as CG numbers although they have been recently *F annotated as PDEs. However, each has a clear similarity to one member *F of vertebrate gene family, so I propose that they are named *F accordingly. *F I have attached the gene list for your consideration. *F Thank you. *F Shireen Davies *F CG no. Closest vertebrate homologue % similarity Proposed Deduced function *F (Accession number) % identity nomenclature *F (Catalytic domain) *F 8279 C. familiaris PDE6 (CAB98144) 173/249: 69% PDE6 cGMP-specific PDE *F 127/249: 51% *F 10231 H. sapiens PDE11a (BAB 16371) 231/241: 96% PDE11 cAMP-, cGMP-specific *F PDE *F 185/241: 77% *F 14940 H. sapiens PDE1C (Q14123) 152/243: 63% PDE1c Ca2+/calmodulin-sensitive *F cGMP-specific PDE *F 193/243: 79% *F 32648 H. sapiens PDE9A (NP002597) 181/238: 76% PDE9 cGMP-specific PDE *F 149/238: 63% *F 5411 H. sapiens PDE8A1 (060658) 224/285: 79% PDE8 cAMP-specific PDE *F 171/285: 60% # *U FBrf0159903 *a Sardiello *b M. *c F. *d Licciulli *e D. *f Catalano *g M. *h Attimonelli *i C. *j Caggese *t 2003.6.11 *T personal communication to FlyBase *u MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion. *F Id/Sim *F MitoDrome ID ENTRY SW-PROT PROTEIN NAME AA(N) *F Drosophila FlyBase ID MAP Mutant *F NAME (HUMAN) HUM/DRO *F GENE POSITION allele *F (%) *F MTDROME10361 KBL O75600 2-AMINO-3-KETOBUTYRATE COENZYME 419/417 *F 66/78 CG10361 FBgn0036208 68E1 *F A LIGASE, MITOCHONDRIAL *F MTDROME07433 GABT P80404 4-AMINOBUTYRATE 500/486 *F 52/71 CG7433 FBgn0036927 76D8-E1 *F AMINOTRANSFERASE, MITOCHONDRIAL *F HEM0 P22557 587/539 *F 59/74 *F MTDROME03017 \------ \--------- 5-AMINOLEVULINIC ACID SYNTHASE, \---------- *F \------ Alas, FBgn0020764 60B8 *F HEM1 P13196 MITOCHONDRIAL 640/539 *F 47/73 CG3017 *F MTDROME10932 THIL P24752 ACETYL-COA ACETYLTRANSFERASE, 427/410 *F 65/80 CG10932 FBgn0029969 7C1 *F MITOCHONDRIAL *F MTDROME04600 THIM P42765 3-KETOACYL-COA THIOLASE, 397/398 *F 59/74 yip2, FBgn0040064 30E4 *F MITOCHONDRIAL *F CG4600 *F Acon, *F MTDROME09244 ACONITATE HYDRATASE, 780/787 *F 70/81 CG9244 FBgn0010100 39B1 *F \------------- ACON Q99798 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- 12 alleles *F MTDROME04706 780/783 *F 70/81 CG4706 FBgn0037862 86D5-6 \----------------------------- *F ACYL-COA DEHYDROGENASE, *F MTDROME12262 ACDM P11310 MEDIUM-CHAIN SPECIFIC, 421/419 *F 70/83 CG12262 FBgn0035811 66A11 *F MITOCHONDRIAL *F ACYL-COA DEHYDROGENASE, *F MTDROME03902 ACDB P45954 SHORT/BRANCHED CHAIN SPECIFIC, 432/414 *F 60/78 CG3902 FBgn0036824 75E4 l(3)j4E6 *F MITOCHONDRIAL *F EP(3)3660 *F Arc42, *F MTDROME04703 ACYL-COA DEHYDROGENASE, 412/405 *F 70/83 CG4703 FBgn0038742 92B4 \----------------------------- *F \------------- ACDS P16219 SHORT-CHAIN SPECIFIC, \---------- *F \------ \--------------- \------------ \---------- BG00476 *F MTDROME04860 MITOCHONDRIAL 412/ 415 *F 57/75 CG4860 FBgn0037999 87B2 *F ACYL-COA DEHYDROGENASE, *F MTDROME07461 ACDV P49748 VERY-LONG-CHAIN SPECIFIC, 655/655 *F 57/72 CG7461 FBgn0034432 56D3 *F MITOCHONDRIAL *F MTDROME03140 KAD2 P54819 ADENYLATE KINASE ISOENZYME 2 238/240 *F 40/62 Adk2, FBgn0022708 60B13 l(2)k04201l(2)k16120EP(2)2149 *F (KAD4) (P27144) (4), MITOCHONDRIAL *F CG3140 *F MTDROME06612 KAD3 Q9UIJ7 GTP:AMP PHOSPHOTRANSFERASE, 226/216 *F 47/64 Adk3, FBgn0042094 86C7 *F MITOCHONDRIAL *F CG6612 *F sesB, *F MTDROME16944 298/299 *F 79/87 CG16944 FBgn0003360 9E10-F1 *F \------------- ADT1 P12235 ADP,ATP CARRIER PROTEIN \---------- *F \------ \--------------- \------------ \---------- 20 alleles *F MTDROME01683 298/307 *F 71/81 Ant2, FBgn0025111 9E10 \----------------------------- *F CG1683 *F MTDROME01319 ADX P10109 ADRENODOXIN, MITOCHONDRIAL 184/153 *F 56/72 CG1319 FBgn0035529 64B1 *F MTDROME06512 AF32 Q9Y4W6 AFG3-LIKE PROTEIN 2 797/826 *F 67/80 CG6512 FBgn0036702 74A1-2 *F MTDROME03752 517/520 *F 72/84 CG3752 FBgn0032114 30B3 *F \------------- DHAM P05091 ALDEHYDE DEHYDROGENASE, \---------- *F \------ \--------------- \------------ \---------- KG02748 *F MTDROME06309 (DHA5) (P30837) MITOCHONDRIAL 517/485 *F 59/74 CG6309 FBgn0039468 97D13-14 \----------------------------- *F MTDROME09319 AMAC Q9UHK6 ALPHA-METHYLACYL-COA RACEMASE 382/373 *F 54/70 CG9319 FBgn0032881 38E5 *F MTDROME06415 GCST P48728 AMINOMETHYLTRANSFERASE, 403/405 *F 54/67 CG6415 FBgn0032287 32A2 *F MITOCHONDRIAL *F MTDROME18104 ARG2 P78540 ARGINASE II 354/351 *F 42/63 arg, FBgn0023535 1B7-8 8 alleles *F CG18104 *F MTDROME12397 BCL2 P10415 APOPTOSIS REGULATOR BCL-2 239/300 *F 22/31 debcl, FBgn0029131 42E-43A *F CG12397 *F 430/424 *F 60/76 *F MTDROME04233 ASPARTATE AMINOTRANSFERASE, 430/431 *F 60/76 CG4233 FBgn0031380 22B8 \----------------------------- *F \------------- AATM P00505 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- BG01081 *F MTDROME08430 430/416 *F 49/70 CG8430 FBgn0034079 53A1 *F MTDROME03156 AB10 Q9NRK6 ATP-BINDING CASSETTE, SUB-FAMILY 738/692 *F 41/55 CG3156 FBgn0023536 1B5 *F B, MEMBER 10, MITOCHONDRIAL *F MTDROME05651 ABE1 Q96B10 ATP-BINDING CASSETTE SUB-FAMILY 599/611 *F 76/86 CG5651 FBgn0035946 65E5 *F E MEMBER 1 *F MTDROME03612 ATPA P25705 ATP SYNTHASE ALPHA CHAIN, 553/552 *F 82/91 blw, FBgn0011211 59B6 22 alleles *F MITOCHONDRIAL *F CG3612 *F MTDROME08189 ATPF P24539 ATP SYNTHASE B CHAIN, 256/243 *F 47/67 ATPsyn-b, FBgn0019644 67C5 *F MITOCHONDRIAL *F CG8189 *F MTDROME11154 *F ATPsyn-beta, *F \------------- ATPB P06576 ATP SYNTHASE BETA CHAIN, 529/505 *F 88/92 CG11154 FBgn0010217 102F3 *F MTDROME05389 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F 529/622 *F 70/82 CG5389 FBgn0036568 72D5-6 *F MTDROME04412 ATPR P18859 ATP SYNTHASE COUPLING FACTOR 6, 108/106 *F 46/68 ATPsyn-Cf6, FBgn0016119 94E13 *F MITOCHONDRIAL *F CG4412 *F MTDROME10731 ATPW Q99766 ATP SYNTHASE COUPLING FACTOR B, 215/203 *F 19/31 CG10731 FBgn0034081 52F2-4 *F MITOCHONDRIAL *F MTDROME06030 ATPQ O75947 ATP SYNTHASE D CHAIN, 160/178 *F 45/64 ATPsyn-d, FBgn0016120 91F1 *F MITOCHONDRIAL *F CG6030 *F MTDROME02968 ATPD P30049 ATP SYNTHASE DELTA CHAIN, 168/157 *F 48/65 CG2968 FBgn0030184 9B4 *F MITOCHONDRIAL *F MTDROME03321 ATPJ P56385 ATP SYNTHASE E CHAIN, 68/81 *F 51/68 CG3321 FBgn0038224 88B7-8 *F MITOCHONDRIAL *F MTDROME09032 50/61 *F 40/62 sun, *F \------------- ATPE P56381 ATP SYNTHASE EPSILON CHAIN, 50/57 *F 40/62 CG9032 FBgn0014391 13F12 2 alleles *F MTDROME12810 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F 50/64 *F 40/62 CG12810 FBgn0040541 85F11-12 *F MTDROME04692 ATPK P56134 ATP SYNTHASE F CHAIN, 93/107 *F 33/55 CG4692 FBgn0035032 60D15 *F MITOCHONDRIAL *F MTDROME06105 ATPN O75964 ATP SYNTHASE G CHAIN, 103/99 *F 54/70 l(2)06225, FBgn0010612 32C4-5 l(2)06225 *F MITOCHONDRIAL *F CG6105 *F MTDROME07610 ATPG P36542 ATP SYNTHASE GAMMA CHAIN, 298/297 *F 58/78 ATPsyn-gamma, FBgn0020235 99B9 *F MITOCHONDRIAL *F CG7610 *F AT91 P05496 136/138 *F 74/82 *F \------ \--------- \---------- *F \------ *F MTDROME01746 AT92 Q06055 ATP SYNTHASE LIPID-BINDING 141/138 *F 70/83 CG1746 FBgn0039830 100B7 EP(3)3264 *F \------ \--------- PROTEIN P1,P2,P3 \---------- *F \------ *F AT93 P48201 142/138 *F 70/82 *F ATP SYNTHASE OLIGOMYCIN *F MTDROME04307 ATPO P48047 SENSITIVITY CONFERRAL PROTEIN 213/ 209 *F 45/70 Oscp, FBgn0016691 88E8-9 *F PRECURSOR, MITOCHONDRIAL *F CG4307 *F MTDROME04225 ABC6 Q9NP58 ATP-BINDING CASSETTE, SUB-FAMILY 842/866 *F 53/69 CG4225 FBgn0038376 89A6 EP(3)3387 *F B, MEMBER 6, MITOCHONDRIAL *F MTDROME07955 ABC7 O75027 ATP-BINDING CASSETTE, SUB-FAMILY 752/709 *F 56/74 CG7955 FBgn0035244 62A8-9 *F B, MEMBER 7, MITOCHONDRIAL 752/606 *F MTDROME01824 ABC8 Q9NUT2 ATP-BINDING CASSETTE, SUB-FAMILY 735/ 761 *F 50/68 CG1824 FBgn0030403 11B2-3 *F B, MEMBER 8, MITOCHONDRIAL *F BIFUNCTIONAL *F MTDROME18466 MTDC P13995 METHYLENETETRAHYDROFOLATE 344/309 *F 50/64 Nmdmc, FBgn0010222 85C6-7 *F DEHYDROGENASE/CYCLOHYDROLASE, 344/303 *F 50/64 CG18466 *F MITOCHONDRIAL *F MTDROME07314 UCP5 O95258 BRAIN MITOCHONDRIAL CARRIER 325/303 *F 58/73 Bmcp, FBgn0036199 68D3 BG02446 *F PROTEIN-1 *F CG7314 *F MTDROME01673 BCAM O15382 BRANCHED-CHAIN AMINO ACID 392/395 *F 42/60 CG1673 FBgn0030482 11F7 EP(X)1023 *F AMINOTRANSFERASE, MITOCHONDRIAL *F BG00310 *F 678/682 *F 678/679 *F CMC1 O75746 678/695 *F MTDROME02139 \------ \--------- CALCIUM-BINDING MITOCHONDRIAL \---------- *F 58/75 Aralar1, FBgn0028646 99F5-6 EP(3)3675 *F CMC2 Q9UJS0 CARRIER PROTEIN ARALAR1-2 675/682 *F CG2139 *F 675/679 *F 675/695 *F MTDROME03057 301/306 *F 50/69 colt, FBgn0019830 23A5 *F \------------- MCAT O43772 CARNITINE/ACYLCARNITINE CARRIER \---------- *F \------ CG3057 \------------ \---------- 6 alleles *F MTDROME03476 PROTEIN, MITOCHONDRIAL 301/299 *F 45/62 \--------------- FBgn0031881 27D4 \----------------------------- *F CG3476 *F MTDROME05265 CACP P43155 CARNITINE O-ACETYLTRANSFERASE 624/643 *F 33/54 CG5265 FBgn0038486 89F1 *F 773/782 *F CPT1 P50416 773/780 *F MTDROME12891 \------ \--------- CARNITINE O-PALMITOYLTRANSFERASE \---------- *F 50/67 CPTI, FBgn0027842 47A11 1 allele *F CPTM Q92523 I, MITOCHONDRIAL 772/782 *F CG12891 *F 772/780 *F MTDROME02107 CPT2 P23786 CARNITINE O-PALMITOYLTRANSFERASE 658/667 *F 47/68 CG2107 FBgn0035383 63A2 *F II, MITOCHONDRIAL *F MTDROME03861 466/522 *F 70/80 CG3861 FBgn0029869 5F3 *F \------------- CISY O75390 CITRATE SYNTHASE, MITOCHONDRIAL 466/464 *F \------ \--------------- \------------ \---------- 1 allele *F MTDROME14740 \---------- *F 49/66 CG14740 FBgn0037988 87A6 \----------------------------- *F 466/478 *F COMPLEMENT COMPONENT 1, Q *F MTDROME06459 MA32 Q07021 SUBCOMPONENT BINDING PROTEIN, 282/263 *F 32/49 CG6459 FBgn0034259 54E9 *F MITOCHONDRIAL *F COMPLEX I *F MTDROME07598 CI30 Q9Y375 INTERMEDIATE-ASSOCIATED PROTEIN 327/296 *F 31/50 CG7598 FBgn0039689 99B9 *F 30, MITOCHONDRIAL *F MTDROME03433 HEM6 P36551 COPROPORPHYRINOGEN III OXIDASE, 354/390 *F 57/69 Coprox, FBgn0021944 27C7 3 alleles *F MITOCHONDRIAL *F CG3433 *F MTDROME12264 NFS1 Q9Y697 CYSTEINE DESULFURASE, 457/462 *F 74/87 CG12264 FBgn0032393 33B7 *F MITOCHONDRIAL *F Cyt-c-p, EP(2)2305 *F MTDROME17903 *F CG17903 FBgn0000409 36A12 EP(2)2049 *F \------------- CYC P00001 CYTOCHROME C 104/108 *F 74/80 \--------------- \------------ \---------- ms(2)04445 *F MTDROME13263 \---------- *F \------ Cyt-c-d, FBgn0000408 36A11 \----------------------------- *F 104/105 *F 65/75 *F CG13263 l(2)k13905 *F MTDROME06922 COXZ Q9Y6N1 CYTOCHROME C OXIDASE ASSEMBLY 276/241 *F 45/57 CG6922 FBgn0031712 25E5 *F PROTEIN COX11, MITOCHONDRIAL *F MTDROME09065 COXS Q14061 CYTOCHROME C OXIDASE COPPER 62/89 *F 44/51 CG9065 FBgn0030610 13A12 *F CHAPERONE *F MTDROME10396 169/176 *F 33/52 CG10396 FBgn0033020 41D1 *F \------------- CX41 P13073 CYTOCHROME C OXIDASE SUBUNIT IV \---------- *F \------ \--------------- \------------ \---------- *F MTDROME10664 (CX42) (Q96KJ9) 169/182 *F 29/43 CG10664 FBgn0032833 38A8 *F MTDROME14724 COXA P20674 CYTOCHROME C OXIDASE POLYPEPTIDE 150/149 *F 41/55 CoVa, FBgn0019624 86F9 *F VA, MITOCHONDRIAL *F CG14724 *F MTDROME11015 129/120 *F 41/54 CG11015 FBgn0031830 26E3 *F \------------- COXB P10606 CYTOCHROME C OXIDASE POLYPEPTIDE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME11043 VB, MITOCHONDRIAL 129/154 *F 26/41 CG11043 FBgn0031831 26E3 *F COXD Q02221 97/109 *F MTDROME17280 \------ \--------- CYTOCHROME C OXIDASE POLYPEPTIDE \---------- *F 47/61 CG17280 FBgn0034877 59F5 *F COXE P12074 VIA, MITOCHONDRIAL 109/109 *F MTDROME18809 COXG P14854 CYTOCHROME C OXIDASE POLYPEPTIDE 85/77 *F 53/69 CG18809 FBgn0042132 18E5 *F VIB *F MTDROME14028 COXH P09669 CYTOCHROME C OXIDASE POLYPEPTIDE 75/77 *F 41/53 cype, FBgn0015031 25D6 l(2)03771 *F VIC *F CG14028 *F MTDROME09603 79/89 *F 34/54 CG9603 FBgn0040529 84F12 *F \------------- COXK P24310 CYTOCHROME C OXIDASE POLYPEPTIDE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME18193 (COXJ) (P14406) VIIA-HEART, MITOCHONDRIAL 79/106 *F 30/46 CG18193 FBgn0037579 84F12 *F MTDROME02249 COXO P15954 CYTOCHROME C OXIDASE POLYPEPTIDE 63/66 *F 46/62 CG2249 FBgn0040773 46E3 *F VIIC *F MTDROME06404 OXA1 Q15070 CYTOCHROME OXIDASE BIOGENESIS 495/442 *F 32/52 BcDNA:GH02220, FBgn0027615 67F1 *F PROTEIN OXA1, MITOCHONDRIAL *F CG6404 *F MTDROME04769 325/307 *F 54/68 CG4769 FBgn0035600 64D1 *F \------------- CY1 P08574 CYTOCHROME C1, HEME PROTEIN \---------- *F \------ \--------------- \------------ \---------- *F MTDROME14508 325/344 *F 45/58 CG14508 FBgn0039651 99A1 *F MTDROME06022 CCHL P53701 CYTOCHROME C-TYPE HEME LYASE 268/281 *F 50/62 CG6022 FBgn0038925 93F13 *F MTDROME07470 P5CS P54886 DELTA 1-PYRROLINE-5-CARBOXYLATE 795/776 *F 55/69 CG7470 FBgn0037146 79B2-3 *F SYNTHETASE *F MTDROME07145 566/574 *F 58/77 CG7145 FBgn0037138 79A3 *F \------------- PUT2 P30038 DELTA-1-PYRROLINE-5-CARBOXYLATE \---------- *F \------ \--------------- \------------ \---------- EP(3)3138 *F MTDROME06661 DEHYDROGENASE 566/581 *F 35/52 CG6661 FBgn0036403 70C15 \----------------------------- *F MTDROME09577 ECH1 Q13011 DELTA3,5-DELTA2,4-DIENOYL-COA 328/312 *F 43/61 CG9577 FBgn0031092 19C1 *F ISOMERASE *F DIHYDROLIPOAMIDE *F MTDROME05261 ODP2 P10515 ACETYLTRANSFERASE COMPONENT OF 614/512 *F 39/51 CG5261 FBgn0031912 27F7 *F PYRUVATE DEHYDROGENASE COMPLEX, 614/421 *F 36/46 *F MITOCHONDRIAL *F MTDROME07430 DLDH P09622 DIHYDROLIPOAMIDE DEHYDROGENASE, 509/504 *F 65/76 CG7430 FBgn0036762 75A4 EP(3)3231 *F MITOCHONDRIAL *F EP(3)3698 *F DIHYDROLIPOAMIDE *F MTDROME05214 ODO2 P36957 SUCCINYLTRANSFERASE COMPONENT OF 453/468 *F 53/67 CG5214 FBgn0037891 86E1 *F 2-OXOGLUTARATE DEHYDROGENASE *F COMPLEX, MITOCHONDRIAL *F MTDROME09741 PYRD Q02127 DIHYDROOROTATE DEHYDROGENASE 369/405 *F 47/63 Dhod, FBgn0000447 85A5 24 alleles *F CG9741 *F MTDROME08987 DPG1 P54098 DNA POLYMERASE GAMMA, SUBUNIT 1 1239/1145 *F 42/57 tam, FBgn0004406 34D6 10 alleles *F CG8987 *F DNApol-gamma35, *F MTDROME08969 DPG2 Q9UHN1 DNA POLYMERASE GAMMA SUBUNIT 2 485/361 *F 16/25 FBgn0004407 34D-E 4 alleles *F CG8969 *F MTDROME04644 RPOM O00411 DNA-DIRECTED RNA POLYMERASE, 1230/1369 *F 37/53 CG4644 FBgn0031300 21E4-F1 *F MITOCHONDRIAL *F MTDROME08996 ETFA P13804 ELECTRON TRANSFER FLAVOPROTEIN 333/330 *F 69/81 wal, FBgn0010516 48C2 5 alleles *F ALPHA-SUBUNIT *F CG8996 *F MTDROME07834 ETFB P38117 ELECTRON TRANSFER FLAVOPROTEIN 255/253 *F 70/80 CG7834 FBgn0039697 99C1 *F BETA-SUBUNIT *F ELECTRON TRANSFER *F MTDROME12140 ETFD Q16134 FLAVOPROTEIN-UBIQUINONE 617/604 *F 64/77 CG12140 FBgn0033465 46C6 *F OXIDOREDUCTASE *F MTDROME06412 EFTS P43897 ELONGATION FACTOR TS, 325/318 *F 33/47 CG6412 FBgn0032646 36C10 *F MITOCHONDRIAL *F MTDROME06050 *F EfTuM, *F \------------- EFTU P49411 ELONGATION FACTOR TU, 452/489 *F 57/72 CG6050 FBgn0024556 50B8 l(3)4569 *F MTDROME12736 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F 452/456 *F 44/63 CG12736 FBgn0033184 43D5 *F MTDROME08862 NUCG Q14249 ENDONUCLEASE G 297/295 *F 48/62 CG8862 FBgn0033690 48F1 *F MTDROME06543 ECHM P30084 ENOYL-COA HYDRATASE, 290/295 *F 56/73 CG6543 FBgn0033879 50C11 l(2)k08708 *F MITOCHONDRIAL *F ferrochelatase, *F MTDROME02098 HEMZ P22830 FERROCHELATASE, MITOCHONDRIAL 423/384 *F 48/65 FBgn0024891 100F5 *F CG2098 *F MTDROME02543 FOLC Q05932 FOLYLPOLYGLUTAMATE SYNTHASE, 587/572 *F 32/46 folc, FBgn0030407 11B7 PG44 *F MITOCHONDRIAL *F CG2543 *F MTDROME08971 FRDA Q16595 FRATAXIN 210/190 *F 34/46 fh, FBgn0030092 8D9 *F CG8971 *F 510/495 *F 72/82 *F MTDROME04094 510/467 *F 70/80 CG4094 FBgn0029889 6C10-11 *F \------------- FUMARATE HYDRATASE, \---------- *F \------ \--------------- \------------ \---------- *F MTDROME04095 FUMH P07954 MITOCHONDRIAL 510/500 *F 67/78 CG4095 6C5 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F MTDROME31874 510/484 *F 54/70 CG31874 FBgn0051874 31B1 *F MTDROME05320 DHE3 P00367 558/549 *F 66/80 Gdh, *F \------------- \------ \--------- GLUTAMATE DEHYDROGENASE , 558/562 *F 65/78 CG5320 FBgn0001098 95C5 5 alleles *F MTDROME04434 DHE4 P49448 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F 558/535 *F 43/61 CG4434 FBgn0039071 94E6 *F 669/706 *F 39/54 *F 669/716 *F 39/54 *F MTDROME08772 GLSK O94925 GLUTAMINASE, ISOFORMS, 669/648 *F 39/54 nemy, FBgn0033765 49C1 *F (GLSL) (Q9UI32) MITOCHONDRIAL *F CG8772 *F 669/658 *F 39/53 *F 669/737 *F 39/52 *F MTDROME09547 GCDH Q92947 GLUTARYL-COA DEHYDROGENASE, 438/419 *F 63/76 CG9547 FBgn0031824 26D1 *F MITOCHONDRIAL *F MTDROME01742 155/151 *F 39/56 Mgstl, *F \------------- GST1 P10620 GLUTATHIONE S-TRANSFERASE 155/152 *F 44/59 CG1742 FBgn0025814 19E6 5 alleles *F MTDROME12628 \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F 155/124 *F 38/50 CG12628 FBgn0032985 40E3 *F MTDROME05508 PLSB Q9HCL2 GLYCEROL-3-PHOSPHATE 828/850 *F 26/47 CG5508 FBgn0027579 98B3-4 *F ACYLTRANSFERASE, MITOCHONDRIAL *F MTDROME08256 727/724 *F 61/75 CG8256 FBgn0022160 52C8 *F \------------- GPDM P43304 GLYCEROL-3-PHOSPHATE \---------- *F \------ \--------------- \------------ \---------- l(2)k05713 *F MTDROME02137 DEHYDROGENASE, MITOCHONDRIAL 727/713 *F 42/64 CG2137 FBgn0033190 43D5 \----------------------------- *F MTDROME07758 GCSH P23434 GLYCINE CLEAVAGE SYSTEM H 173/165 *F 45/58 ppl, FBgn0027945 78C2 8 alleles *F PROTEIN, MITOCHONDRIAL *F CG7758 *F GLYCINE DEHYDROGENASE *F MTDROME03999 GCSP P23378 DECARBOXYLATING, 1020/985 *F 59/72 CG3999 FBgn0037801 86A1 *F MITOCHONDRIAL *F MTDROME06155 GRE2 Q8TAA5 GRPE PROTEIN HOMOLOG 2, 225/213 *F 35/54 Roe1, FBgn0014877 50B1 *F MITOCHONDRIAL *F CG6155 *F MTDROME11267 101/103 *F 53/70 CG11267 FBgn0036334 69F2 *F \------------- CH10 Q04984 10 KDA HEAT SHOCK PROTEIN, \---------- *F \------ \--------------- \------------ \---------- *F MTDROME09920 MITOCHONDRIAL 101/102 *F 49/65 CG9920 FBgn0038200 88A4 *F Hsp60, *F MTDROME12101 *F CG12101 FBgn001524 10A8 *F \------------- 573/573 *F 74/85 \--------------- \------------ \---------- 53 alleles *F MTDROME07235 \---------- *F \------ CG7235 FBgn0031728 25F1 \----------------------------- *F \------------- CH60 P10809 60 KDA HEAT SHOCK PROTEIN, *F \--------------- \------------ \---------- *F MTDROME02830 MITOCHONDRIAL \---------- *F \------ Hsp60B, \----------------------------- *F CG2830 FBgn0011244 21D2 *F \------------- \---------- *F \------ \--------------- \------------ \---------- 06619 *F MTDROME16954 *F CG16954 FBgn0032525 34B7 \----------------------------- *F Hsc70-5, *F MTDROME08542 679/686 *F CG8542 FBgn0001220 50E6 k04907 *F \------------- \---------- *F \--------------- \------------ \---------- k06618 *F 72/83 Hsc70-3, \----------------------------- *F MTDROME04147 6.0e-161 *F \------ CG4147 FBgn0001218 10F1 *F \------------- \---------- *F \--------------- \------------ \---------- 14 alleles *F \------ Hsc70-4, \----------------------------- *F MTDROME04264 3.2e-153 *F CG4264 FBgn0001219 88E2 *F \------------- GR75 P38646 MITOCHONDRIAL STRESS-70 PROTEIN \---------- *F \------ \--------------- \------------ \---------- 30 alleles *F Hsp68, \----------------------------- *F MTDROME05436 5.5e-151 *F \------ CG5436 FBgn0001230 95D3 *F \------------- \---------- *F \--------------- \------------ \---------- 5 alleles *F \------ Hsc70-1, \----------------------------- *F MTDROME08937 1.7e-149 *F CG8937 FBgn0001216 70C5 *F \------------- \---------- *F \--------------- \------------ \---------- \----------------------------- *F Hsc70-2, *F MTDROME07756 1.8e-143 *F CG7756 FBgn0001217 87D5-6 *F MTDROME15093 D3HI P31937 3-HYDROXYISOBUTYRATE 336/324 *F 46/63 CG15093 FBgn0034390 55F2 *F DEHYDROGENASE, MITOCHONDRIAL *F MTDROME10399 HMGL P35914 HYDROXYMETHYLGLUTARYL-COA LYASE, 325/323 *F 58/73 CG10399 FBgn0031877 27C7 *F MITOCHONDRIAL *F IMPORT INNER MEMBRANE *F MTDROME09878 IM10 Q9Y5J8 TRANSLOCASE SUBUNIT TIM10, 90/92 *F 56/72 Tim10, FBgn0027360 57F8 4 alleles *F MITOCHONDRIAL *F CG9878 *F I13A Q9UHL8 IMPORT INNER MEMBRANE 95/92 *F 49/63 *F MTDROME11611 \------ \--------- TRANSLOCASE SUBUNIT TIM13 A-B, \---------- *F \------ Tim13, FBgn0036204 68D4 *F I13B Q9Y5L4 MITOCHONDRIAL 95/92 *F 49/64 CG11611 *F Tim17a1, *F MTDROME10090 IMPORT INNER MEMBRANE 171/222 *F 38/49 CG10090 FBgn0038018 87B7 *F \------------- IM7A Q9959 TRANSLOCASE SUBUNIT TIM17 A, \---------- *F \------ \--------------- \------------ \---------- *F MITOCHONDRIAL 171/204 *F 29/40 Tim17a2, *F MTDROME14666 *F CG14666 FBgn0037307 82F8 *F Tim17b1, *F MTDROME01158 172/179 *F 48/58 CG1158 FBgn0037310 82F9 *F \------------- IMPORT INNER MEMBRANE \---------- *F \------ \--------------- \------------ \---------- *F IM7B O60830 TRANSLOCASE SUBUNIT TIM17 B, *F Tim17b2, *F MTDROME15257 MITOCHONDRIAL 172/176 *F 56/72 CG15257 FBgn0020371 35D2 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F MTDROME01724 172/185 *F 55/70 CG1724 FBgn0031164 19F1 *F IMPORT INNER MEMBRANE *F MTDROME31229 IM22 Q9Y584 TRANSLOCASE SUBUNIT TIM22, 194/195 *F 42/59 CG31229 91B8 *F MITOCHONDRIAL *F IMPORT INNER MEMBRANE *F MTDROMETim23 IM23 O14925 TRANSLOCASE SUBUNIT TIM23, 209/206 *F 39/51 Tim23 FBgn0063893 *F MITOCHONDRIAL *F IM8A O60220 IMPORT INNER MEMBRANE 97/88 *F 35/47 *F MTDROME01728 \------ \--------- TRANSLOCASE SUBUNIT TIM8 A, \---------- *F \------ Tim8, FBgn0027359 10B10 *F IM8B Q9Y5J9 MITOCHONDRIAL 83/88 *F 51/68 CG1728 *F IMPORT INNER MEMBRANE *F MTDROME01660 IM9A Q9Y5J7 TRANSLOCASE SUBUNIT TIM9 A, 89/95 *F 49/65 Tim9a, FBgn0030480 11F5 *F MITOCHONDRIAL *F CG1660 *F IMPORT INNER MEMBRANE *F MTDROME17767 IM9B Q9Y5J6 TRANSLOCASE SUBUNIT TIM9 B, 103/117 *F 30/43 Tim9b, FBgn0027358 18D7-8 *F MITOCHONDRIAL *F CG17767 *F MTDROME07654 145/171 *F 47/62 CG7654 FBgn0036928 76E1 *F \------------- OM20 Q15388 IMPORT RECEPTOR SUBUNIT TOM20 \---------- *F \------ \--------------- \------------ \---------- *F MTDROME14690 HOMOLOG, MITOCHONDRIAL 145/147 *F 41/61 CG14690 FBgn0037828 86C5 *F IMPORT INNER MEMBRANE *F MTDROME11779 IM44 O43615 TRANSLOCASE SUBUNIT TIM44, 452/459 *F 45/67 CG11779 FBgn0038683 91F6-7 3 alleles *F MITOCHONDRIAL 452/428 *F 48/71 *F MTDROME07791 PMIP Q99797 INTERMEDIATE PEPTIDASE, 713/699 *F 46/64 CG7791 FBgn0033038 41F9 KG05092 *F MITOCHONDRIAL *F MTDROME12233 IDHA P50213 ISOCITRATE DEHYDROGENASE NAD 366/354 *F 67/80 CG12233 FBgn0031024 18C8 PG140 *F SUBUNIT ALPHA, MITOCHONDRIAL 366/377 *F 63/76 *F MTDROME06439 IDHB O43837 ISOCITRATE DEHYDROGENASE NAD 385/370 *F 57/74 CG6439 FBgn0038922 93F13 *F SUBUNIT BETA, MITOCHONDRIAL *F MTDROME05028 IDHG P51553 ISOCITRATE DEHYDROGENASE NAD 393/402 *F 43/60 CG5028 FBgn0039358 96E4 *F SUBUNIT GAMMA, MITOCHONDRIAL *F 452/416 *F 58/71 *F 452/416 *F 58/71 *F MTDROME07176 IDHP P48735 ISOCITRATE DEHYDROGENASE NADP, 452/450 *F 58/71 CG7176 FBgn0001248 66C7 8 alleles *F MITOCHONDRIAL *F 452/437 *F 57/70 *F 452/427 *F 58/72 *F MTDROME06638 IVD P26440 ISOVALERYL-COA DEHYDROGENASE, 423/420 *F 60/73 CG6638 FBgn0035911 66D6-7 *F MITOCHONDRIAL *F LIPOAMIDE ACYLTRANSFERASE *F MTDROME05599 ODB2 P11182 COMPONENT OF BRANCHED-CHAIN 482/462 *F 45/61 CG5599 FBgn0030612 13A10 *F ALPHA-KETO ACID DEHYDROGENASE *F COMPLEX, MITOCHONDRIAL *F MTDROME08798 LONM P36776 LON PROTEASE HOMOLOG, 959/1006 *F 56/69 CG8798 FBgn0036892 76B9 *F MITOCHONDRIAL *F MTDROME07998 338/336 *F 59/77 CG7998 FBgn0038587 90F6 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F MTDROME10749 MDHM P40926 MALATE DEHYDROGENASE, 338/347 *F 45/63 CG10749 FBgn0036328 69F2 *F \------------- MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F MTDROME10748 338/349 *F 42/60 CG10748 FBgn0036327 69F2 *F MTDROME09393 MTX1 Q13505 METAXIN 1 317/327 *F 29/48 CG9393 FBgn0037710 85D24-25 *F MTDROME08004 263/269 *F 41/61 CG8004 FBgn0036920 76D5 *F \------------- MTX2 O75431 METAXIN 2 \---------- *F \------ \--------------- \------------ \---------- *F MTDROME05662 263/292 *F 32/49 CG5662 FBgn0030620 13B3 *F MTDROME02118 MCCA Q96RQ3 METHYLCROTONYL-COA CARBOXYLASE 725/634 *F 48/62 CG2118 FBgn0039877 100D2 *F ALPHA CHAIN, MITOCHONDRIAL 725/698 *F 51/66 *F MTDROME03267 MCCB Q9HCC0 METHYLCROTONYL-COA CARBOXYLASE 563/578 *F 67/78 CG3267 FBgn0042083 42C7 *F ALPHA CHAIN, MITOCHONDRIAL *F METHYLMALONATE-SEMIALDEHYDE *F MTDROME17896 MMSA Q02252 DEHYDROGENASE ACYLATING, 535/552 *F 61/77 CG17896 FBgn0023537 1B5-6 EP(X)1320 *F MITOCHONDRIAL 535/511 *F 64/80 *F MTDROME08026 MFT Q9H2D1 MITOCHONDRIAL FOLATE 315/304 *F 52/69 CG8026 FBgn0033391 45B3 *F TRANSPORTER/CARRIER *F MTDROME01628 ORT1 Q9Y619 MITOCHONDRIAL ORNITHINE 301/459 *F 30/42 CG1628 FBgn0030218 9D3-4 *F TRANSPORTER 1 *F MTDROME05705 RF1M O75570 MITOCHONDRIAL PEPTIDE CHAIN 445/392 *F 34/50 CG5705 FBgn0032486 34A10 *F RELEASE FACTOR 1 *F MTDROME06756 OM70 O94826 MITOCHONDRIAL PRECURSOR PROTEINS 608/589 *F 37/56 CG6756 FBgn0032397 33B9-10 2 alleles *F IMPORT RECEPTOR *F MTDROME08728 MPPA Q10713 MITOCHONDRIAL PROCESSING 525/556 *F 48/64 CG8728 FBgn0033235 43F3-4 *F PEPTIDASE ALPHA SUBUNIT *F MTDROME03731 MPPB O75439 MITOCHONDRIAL PROCESSING 489/470 *F 65/74 CG3731 FBgn0038271 88D5 *F PEPTIDASE BETA SUBUNIT *F MTDROME05889 MAON Q16798 NADP-DEPENDENT MALIC ENZYME, 604/617 *F 52/68 Mdh, FBgn002915 *F MITOCHONDRIAL *F CG5889 97E10 EP(3)3349 *F MTDROME08680 NUMM O75380 NADH-UBIQUINONE OXIDOREDUCTASE 124/126 *F 48/58 CG8680 FBgn0031684 25C6 *F 13 KDA-A SUBUNIT *F MTDROME06463 NUFM Q16718 NADH-UBIQUINONE OXIDOREDUCTASE 115/124 *F 46/61 CG6463 FBgn0036100 67E7 *F 13 KDA-B SUBUNIT *F MTDROME11455 NIPM O43920 NADH-UBIQUINONE OXIDOREDUCTASE 105/101 *F 25/37 CG11455 FBgn0031228 21B1-2 *F 15 KDA SUBUNIT *F MTDROME12203 NUYM O43181 NADH-UBIQUINONE OXIDOREDUCTASE 175/183 *F 44/62 CG12203 FBgn0031021 18C7 *F 18 KDA SUBUNIT *F MTDROME03683 NUPM P51970 NADH-UBIQUINONE OXIDOREDUCTASE 171/175 *F 44/57 CG3683 FBgn0035046 60E1 *F 19 KDA SUBUNIT *F MTDROME09172 213/221 *F 60/73 CG9172 FBgn0030718 14A5 *F \------------- NUKM O75251 NADH-UBIQUINONE OXIDOREDUCTASE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME02014 20 KDA SUBUNIT 213/212 *F 64/74 CG2014 FBgn0039669 99B1 *F MTDROME03944 NUIM O00217 NADH-UBIQUINONE OXIDOREDUCTASE 210/217 *F 69/75 ND23, FBgn0017567 89A5 *F 23 KDA SUBUNIT *F CG3944 *F MTDROME05703 249/242 *F 64/78 CG5703 FBgn0030853 16B10 *F \------------- NUHM P19404 NADH-UBIQUINONE OXIDOREDUCTASE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME06485 24 KDA SUBUNIT 249/238 *F 40/57 CG6485 FBgn0036706 74A4 *F MTDROME12079 NUGM O75489 NADH-UBIQUINONE OXIDOREDUCTASE 264/265 *F 62/72 CG12079 FBgn0035404 63B5 *F 30 KDA SUBUNIT *F MTDROME06020 NUEM Q16795 NADH-UBIQUINONE OXIDOREDUCTASE 377/416 *F 42/57 CG6020 FBgn0037001 77C6 *F 39 KDA SUBUNIT *F MTDROME06343 NUDM O95299 NADH-UBIQUINONE OXIDOREDUCTASE 3.4e-48 *F 30/50 ND42, FBgn0019957 93F13 *F 42 KDA SUBUNIT, MITOCHONDRIAL *F CG6343 *F MTDROME01970 463/468 *F 74/84 CG1970 FBgn0039909 102C4 *F \------------- NUCM O75306 NADH-UBIQUINONE OXIDOREDUCTASE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME11913 49 KDA SUBUNIT 463/502 *F 54/67 CG11913 FBgn0039331 96D2 *F MTDROME09140 464/474 *F 78/87 CG9140 FBgn0031771 26B6 *F \------------- NUBM P49821 NADH-UBIQUINONE OXIDOREDUCTASE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME08102 51 KDA SUBUNIT 464/462 *F 45/59 CG8102 FBgn0034007 51F12 *F 464/481 *F 48/62 *F MTDROME02286 NUAM P28331 NADH-UBIQUINONE OXIDOREDUCTASE 727/731 *F 60/74 ND75, FBgn0017566 7E1 *F 75 KDA SUBUNIT *F CG2286 *F MTDROME03192 NIAM O95169 NADH-UBIQUINONE OXIDOREDUCTASE 186/175 *F 34/46 CG3192 FBgn0029888 6C5 *F ASHI SUBUNIT *F MTDROME10320 NB2M O43676 NADH-UBIQUINONE OXIDOREDUCTASE 97/110 *F 33/43 CG10320 FBgn0034645 57F6 *F B12 SUBUNIT *F MTDROME07712 NB4M P56556 NADH-UBIQUINONE OXIDOREDUCTASE 127/124 *F 47/72 CG7712 FBgn0033570 47C6 *F B14 SUBUNIT *F MTDROME12859 NB5M O95168 NADH-UBIQUINONE OXIDOREDUCTASE 128/113 *F 21/38 CG12859 FBgn0033961 51C2 *F B15 SUBUNIT *F MTDROME13240 NB7M O95139 NADH-UBIQUINONE OXIDOREDUCTASE 167/127 *F 18/33 CG13240 FBgn0028508 35D3 *F B17 SUBUNIT *F MTDROME05548 NB8M P17568 NADH-UBIQUINONE OXIDOREDUCTASE 136/117 *F 41/53 CG5548 FBgn0030605 13A8 *F B18 SUBUNIT *F MTDROME09306 NI2M Q9Y6M9 NADH-UBIQUINONE OXIDOREDUCTASE 178/144 *F 35/50 CG9306 FBgn0032511 34B8 *F B22 SUBUNIT *F MTDROME15434 NI8M O43678 NADH-UBIQUINONE OXIDOREDUCTASE 95/98 *F 42/59 CG15434 FBgn0040705 24F3 *F B8 SUBUNIT *F MTDROME32230 NUML O00483 NADH-UBIQUINONE OXIDOREDUCTASE 81/83 *F 50/62 CG32230 FBgn0052230 80E2 *F (NUOS) (Q9NRX3) MLRQ SUBUNIT *F MTDROME18624 NINM O75438 NADH-UBIQUINONE OXIDOREDUCTASE 58/56 *F 39/53 CG18624 FBgn0029971 7D1 *F MNLL SUBUNIT *F MTDROME08844 NIDM O96000 NADH-UBIQUINONE OXIDOREDUCTASE 171/159 *F 30/51 Pdsw, FBgn0021967 23F3 *F PDSW SUBUNIT *F CG8844 *F MTDROME09762 NISM O43674 NADH-UBIQUINONE OXIDOREDUCTASE 189/186 *F 32/43 l(3)neo18, FBgn0011455 68F5 *F SGDH SUBUNIT *F CG9762 *F MTDROME03621 N4AM O95182 NADH-UBIQUINONE OXIDOREDUCTASE 112/103 *F 40/53 CG3621 FBgn0025839 2D6-E1 *F SUBUNIT B14.5A *F MTDROME12400 N4BM O95298 NADH-UBIQUINONE OXIDOREDUCTASE 119/116 *F 30/53 CG12400 FBgn0031505 23D3 *F SUBUNIT B14.5B *F MTDROME03446 NB6M Q9P0J0 NADH-UBIQUINONE OXIDOREDUCTASE 143/154 *F 34/50 CG3446 FBgn0029868 5F2 *F SUBUNIT B16.6 *F MTDROME03214 N7BM Q9UI09 NADH-UBIQUINONE OXIDOREDUCTASE 145/142 *F 38/55 CG3214 FBgn0031436 23A1 *F SUBUNIT B17.2 *F NADH-UBIQUINONE OXIDOREDUCTASE, *F MTDROME09160 ACPM O14561 ACYL CARRIER PROTEIN, 132/143 *F 47/60 MtACP1, FBgn0011361 61F6 j4A6 *F MITOCHONDRIAL 132/152 *F 46/55 CG9160 *F MTDROME12390 ADRO P22570 NADPH:ADRENODOXIN 491/466 *F 45/60 dare, FBgn0015582 47E4 10 alleles *F OXIDOREDUCTASE, MITOCHONDRIAL 491/426 *F 40/54 CG12390 *F MTDROME17320 NLTP P22307 NONSPECIFIC LIPID-TRANSFER 547/544 *F 60/74 Scpx, FBgn0015808 36F11-37A1 *F PROTEIN *F CG17320 *F MTDROME10214 ORN Q9Y3B8 OLIGORIBONUCLEASE, MITOCHONDRIAL 237/211 *F 42/59 CG10214 FBgn0039115 95A7 *F MTDROME08782 OAT P04181 ORNITHINE AMINOTRANSFERASE 439/431 *F 69/83 CG8782 FBgn0036898 76B8 *F MTDROME11661 1002/1008 *F 61/74 CG11661 FBgn0036682 73D5-6 *F \------------- ODO1 Q02218 2-OXOGLUTARATE DEHYDROGENASE E1 \---------- *F \------ \--------------- \------------ \---------- *F MTDROME07934 COMPONENT, MITOCHONDRIAL 1002/1238 *F 43/56 CG7934 FBgn0035240 62A7-8 *F 1002/1260 *F 42/55 *F MTDROME01907 313/317 *F 56/68 CG1907 FBgn0039674 99B2 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F MTDROME18418 M2OM Q02978 2-OXOGLUTARATE/MALATE CARRIER 313/311 *F 52/68 CG18418 FBgn0035568 64B15 *F \------------- PROTEIN, MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F MTDROME07514 313/301 *F 51/63 CG7514 FBgn0035567 64B15 *F MTDROME08199 ODBA P12694 2-OXOISOVALERATE DEHYDROGENASE 445/439 *F 54/69 CG8199 FBgn0037709 85D22 *F ALPHA SUBUNIT, MITOCHONDRIAL *F MTDROME17691 ODBB P21953 2-OXOISOVALERATE DEHYDROGENASE 392/364 *F 56/70 CG17691 FBgn0039993 *F BETA SUBUNIT *F MTDROME02658 PGN Q9UQ90 PARAPLEGIN 795/819 *F 46/61 EG:100G10.7, FBgn0024992 3B2-3 4 alleles *F CG2658 *F MTDROME02789 PKBS P30536 PERIPHERAL-TYPE BENZODIAZEPINE 169/185 *F 38/51 CG2789 FBgn0031263 21D1 *F RECEPTOR *F MTDROME09090 362/374 *F 66/79 CG9090 FBgn0034497 56F15 *F \------------- MPCP Q00325 PHOSPHATE CARRIER PROTEIN, \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F MTDROME04994 MITOCHONDRIAL *F Mpcp, 00564 *F 362/356 *F 67/78 CG4994 FBgn0026409 70E1 04680 *F MTDROME17725 *F pepck, *F \------------- PHOSPHOENOLPYRUVATE 640/647 *F 60/74 CG17725 FBgn0003067 55D3 *F PPCM Q16822 CARBOXYKINASE, MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F MTDROME10924 PRECURSOR GTP 640/563 *F 60/68 *F 640/638 *F 64/74 CG10924 FBgn0034356 55D1-2 *F PHOSPHOLIPID HYDROPEROXIDE 197/169 *F 38/56 *F MTDROME12013 GSHH P36969 GLUTATHIONE PEROXIDASE, 197/198 *F 35/52 CG12013 FBgn0035438 63D1 *F MITOCHONDRIAL 197/238 *F 35/53 *F PROBABLE GLUTAMYL-TRNA(GLN) *F MTDROME05463 GATB O75879 AMIDOTRANSFERASE SUBUNIT B, 557/514 *F 38/53 CG5463 FBgn0039153 95D9-10 *F MITOCHONDRIAL *F MTDROME07479 SYLM Q15031 PROBABLE LEUCYL-TRNA SYNTHETASE, 903/869 *F 40/54 CG7479 FBgn0035576 64C2 *F MITOCHONDRIAL *F MTDROME12157 *F Tom40, *F \------------- OM40 O96008 PROBABLE MITOCHONDRIAL IMPORT 361/344 *F 52/66 CG12157 FBgn0016041 7B8-C1 *F MTDROME08330 RECEPTOR SUBUNIT TOM40 HOMOLOG \---------- *F \------ \--------------- \------------ \---------- *F 361/340 *F 44/59 CG8330 FBgn0033074 42A14 *F MTDROME08226 OM07 Q9P0U1 PROBABLE MITOCHONDRIAL IMPORT 55/54 *F 49/64 CG8226 FBgn0033357 44F11 *F RECEPTOR SUBUNIT TOM7 HOMOLOG *F MTDROME07263 PCD8 O95831 PROGRAMED CELL DEATH PROTEIN 8, 613/739 *F 48/66 CG7263 FBgn0031392 22D1 *F MITOCHONDRIAL 613/674 *F 48/66 *F MTDROME01417 PROD O43272 PROLINE OXIDASE, MITOCHONDRIAL 516/669 *F 39/51 slgA, FBgn0003423 19F3-4 4 alleles *F CG1417 *F PROTOHEME IX *F MTDROME05037 COXX Q12887 FARNESYLTRANSFERASE, 443/391 *F 37/47 CG5037 FBgn0032222 31B1 *F MITOCHONDRIAL *F MTDROME05796 PPOX P50336 PROTOPORPHYRINOGEN OXIDASE 477/475 *F 38/56 CG5796 FBgn0020018 96A9 *F ATP-DEPENDENT CLP PROTEASE *F MTDROME04538 CLPX O76031 ATP-BINDING SUBUNIT CLPX, 633/673 *F 49/59 CG4538 FBgn0038745 92B3 *F MITOCHONDRIAL *F PUTATIVE ATP-DEPENDENT CLP *F MTDROME05045 CLPP Q16740 PROTEASE PROTEOLYTIC SUBUNIT, 277/253 *F 49/58 CG5045 FBgn0032229 31C1-2 *F MITOCHONDRIAL *F MTDROME01516 PYC P11498 PYRUVATE CARBOXYLASE 1178/1181 *F 67/81 BcDNA-GH06348, FBgn0027580 46B9-12 EP2547 *F 1178/542 *F 30/37 CG1516 *F ODPA P08559 PYRUVATE DEHYDROGENASE E1 390/399 *F 54/68 *F MTDROME07010 \------ \--------- COMPONENT ALPHA SUBUNIT, \---------- *F \------ CG7010 FBgn0029721 4D1 *F ODPT P29803 MITOCHONDRIAL 388/443 *F 47/60 *F PYRUVATE DEHYDROGENASE E1 *F MTDROME11876 ODPB P11177 COMPONENT BETA SUBUNIT, 359/365 *F 64/77 CG11876 FBgn0039635 98F5 *F MITOCHONDRIAL 359/273 *F 34/47 *F PDK1 Q15118 436/413 *F 50/66 *F \------ \--------- \---------- *F \------ *F PDK2 Q15119 PYRUVATE DEHYDROGENASE 407/413 *F 54/70 *F MTDROME08808 \------ \--------- LIPOAMIDE KINASE ISOZYME 1, \---------- *F \------ Pdk, FBgn0017558 45D5-6 4018 *F PDK3 Q15120 2, 3, 4, MITOCHONDRIAL 406/413 *F 55/68 CG8808 EP547 *F \------ \--------- \---------- *F \------ *F PDK4 Q16654 411/413 *F 51/67 *F PDP1 Q9P0J1 PYRUVATE DEHYDROGENASE 538/475 *F 33/51 *F MTDROME12151 \------ \--------- LIPOAMIDE-PHOSPHATASE 1, 2, \---------- *F \------ CG12151 FBgn0029958 7B8 *F PDP2 Q9P2J9 MITOCHONDRIAL 529/475 *F 34/47 *F MTDROME15016 RT06 P82932 28S RIBOSOMAL PROTEIN S6, 124/147 *F 35/51 mRpS6, FBgn0035534 64B3 *F MITOCHONDRIAL *F CG15016 *F MTDROME07925 RT12 O15235 28S RIBOSOMAL PROTEIN S12, 138/140 *F 53/61 tko, FBgn0003714 3A3 16 alleles *F MITOCHONDRIAL 138/154 *F 49/56 CG7925 *F MTDROME04207 RT15 P82914 28S RIBOSOMAL PROTEIN S15, 257/280 *F 22/35 bonsai, FBgn0026261 58F3 k08322 *F MITOCHONDRIAL *F CG4207 *F MTDROME08338 RT16 Q9Y3D3 28S RIBOSOMAL PROTEIN S16, 137/129 *F 41/55 mRpS16, FBgn0033907 50E1 *F MITOCHONDRIAL *F CG8338 *F MTDROME04326 RT17 Q9Y2R5 28S RIBOSOMAL PROTEIN S17, 130/155 *F 23/39 mRpS17, FBgn0034986 60C1 *F MITOCHONDRIAL *F CG4326 *F MTDROMERpS21 RT21 P82921 28S RIBOSOMAL PROTEIN S21, 87/87 *F 49/74 mRpS21 FBgn0044511 *F MITOCHONDRIAL *F MTDROME12261 RT22 P82650 28S RIBOSOMAL PROTEIN S22, 360/393 *F 26/44 mRpS22, FBgn0039555 98B3 *F MITOCHONDRIAL *F CG12261 *F MTDROME14413 RT25 P82663 28S RIBOSOMAL PROTEIN S25, 173/167 *F 54/70 mRpS25, FBgn0030572 12F1 *F MITOCHONDRIAL *F CG14413 *F MTDROME03633 RT29 P51398 28S RIBOSOMAL PROTEIN S29, 398/392 *F 36/55 mRpS29, FBgn0034727 58E1 *F MITOCHONDRIAL *F CG3633 *F MTDROME08288 RM03 P09001 60S RIBOSOMAL PROTEIN L3, 348/362 *F 37/52 mRpL3, FBgn0030686 13E16 *F MITOCHONDRIAL *F CG8288 *F MTDROME05012 RM12 P52815 60S RIBOSOMAL PROTEIN L7/L12, 198/182 *F 34/49 mRpL7-L12, FBgn0011787 66E3 5 alleles *F MITOCHONDRIAL *F CG5012 *F SCO1 O75880 300/251 *F 40/54 *F MTDROME08885 \------ \--------- SCO1, SCO2 PROTEIN HOMOLOG \---------- *F \------ CG8885 FBgn0031656 25B1 *F SCO2 O43819 266/251 *F 41/58 *F MTDROME03011 GLYM P34897 SERINE HYDROXYMETHYLTRANSFERASE, 504/537 *F 54/70 CG3011 FBgn0029823 5D1 *F MITOCHONDRIAL 504/467 *F 55/71 *F MTDROME04337 SSB Q04837 SINGLE-STRANDED DNA-BINDING 148/146 *F 37/58 mtSSB, FBgn0010438 89B9 3 alleles *F PROTEIN, MITOCHONDRIAL *F CG4337 *F SUCCINATE DEHYDROGENASE *F MTDROME10219 DHSD O14521 UBIQUINONE CYTOCHROME B SMALL 159/182 *F 27/47 CG10219 FBgn0039112 95A7 *F SUBUNIT, MITOCHONDRIAL *F SUCCINATE DEHYDROGENASE *F MTDROME05718 DHSA P31040 UBIQUINONE FLAVOPROTEIN 664/661 *F 74/84 Scs-fp, FBgn0036222 68E3 *F SUBUNIT, MITOCHONDRIAL *F CG5718 *F SUCCINATE DEHYDROGENASE *F MTDROME03283 DHSB P21912 UBIQUINONE IRON-SULFUR 280/297 *F 65/78 SdhB, FBgn0014028 42D1 *F PROTEIN, MITOCHONDRIAL *F CG3283 *F SUCCINATE DEHYDROGENASE *F MTDROME06666 C560 Q99643 CYTOCHROME B560 SUBUNIT, 169/171 *F 36/55 CG6666 FBgn0037873 86D8 *F MITOCHONDRIAL *F MTDROME04685 SSDH P51649 SUCCINATE SEMIALDEHYDE 535/509 *F 49/66 CG4685 FBgn0039349 96E3 *F DEHYDROGENASE *F MTDROME01065 SUCCINYL-COA LIGASE *F Scsalpha, *F \------------- SUCA P53597 GDP-FORMING ALPHA-CHAIN, 333/328 *F 66/83 CG1065 FBgn0004888 63F1 *F MTDROME06255 MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F 333/342 *F 57/74 CG6255 FBgn0038708 92A3 *F SUCCINYL-COA LIGASE *F MTDROME10622 SCB2 Q96I99 GDP-FORMING BETA-CHAIN, 432/416 *F 54/70 Sucb, FBgn0029118 64D3 *F MITOCHONDRIAL *F CG10622 *F SUCCINYL-COA LIGASE *F MTDROME11963 SCB1 Q9P2R7 ADP-FORMING BETA-CHAIN, 463/502 *F 49/65 CG11963 FBgn0037643 85B7 *F MITOCHONDRIAL *F MTDROME01140 SCOT P55809 SUCCINYL-COA:3-KETOACID-COENZYME 520/516 *F 59/75 CG1140 FBgn0035298 62B11-12 *F A TRANSFERASE, MITOCHONDRIAL *F MTDROME07280 SUOX P51687 SULFITE OXIDASE 488/573 *F 43/57 CG7280 FBgn0030966 17E8 *F MTDROME08905 SODM P04179 SUPEROXIDE DISMUTASE MN, 222/217 *F 57/72 Sod2, FBgn0010213 53C15 KG06854 *F MITOCHONDRIAL *F CG8905 *F MTDROME09943 SUR1 Q15526 SURFEIT LOCUS PROTEIN 1 300/300 *F 42/55 Surf1, FBgn0029117 65D3 *F CG9943 *F MTDROME08517 166/145 *F 33/53 CG8517 FBgn0034472 56E6 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F MTDROME08993 THI2 Q99757 THIOREDOXIN, MITOCHONDRIAL 166/142 *F 33/56 CG8993 FBgn0035334 62D7 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F \----------------------------- *F MTDROME03719 166/160 *F 31/50 CG3719 FBgn0024986 2A1 PG118 *F MTDROME05826 PDX3 P30048 THIOREDOXIN-DEPENDENT PEROXIDE 256/234 *F 50/63 Prx5037, FBgn0038519 90B1 *F REDUCTASE, MITOCHONDRIAL *F CG5826 *F MTDROME05452 KITM O00142 THYMIDINE KINASE 2, 232/250 *F 39/55 dnk, FBgn0022338 91D4 *F MITOCHONDRIAL *F CG5452 *F MTDROME04217 TFAM Q00059 TRANSCRIPTION FACTOR 1, 246/284 *F 24/40 TFAM, FBgn0038805 92E12 *F MITOCHONDRIAL 246/257 *F 28/46 CG4217 *F MTDROME04598 302/281 *F 35/53 CG4598 FBgn0032160 30D1 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F MTDROME04594 D3D2 P42126 3,2-TRANS-ENOYL-COA ISOMERASE, 302/280 *F 35/50 CG4594 FBgn0032161 30D1 *F \------------- MITOCHONDRIAL \---------- *F \------ \--------------- \------------ \---------- *F MTDROME04592 302/287 *F 34/52 CG4592 FBgn0032162 30D1 *F MTDROME12413 IF2M P46199 TRANSLATION INITIATION FACTOR 727/696 *F 46/62 CG12413 FBgn0039588 98D2 *F IF-2, MITOCHONDRIAL *F MTDROME31305 TXTP P53007 TRICARBOXYLATE TRANSPORT 311/317 *F 62/75 CG31305 FBgn0037912 86E12-13 EP3364 *F PROTEIN *F MTDROME04389 ECHA P40939 TRIFUNCTIONAL ENZYME ALPHA 763/783 *F 54/71 CG4389 FBgn0028479 30B8 *F SUBUNIT, MITOCHONDRIAL 763/744 *F 54/70 *F MTDROME04581 ECHB P55084 TRIFUNCTONAL ENZYME BETA 474/469 *F 64/77 thiolase, FBgn0025352 60A5 00628 *F SUBUNIT, MITOCHONDRIAL *F CG4581 k09828 *F MTDROME00000 UCRH P07919 UBIQUINOL-CYTOCHROME C REDUCTASE 91/85 *F 36/54 Ucrh no Flybase *F COMPLEX 11 KDA PROTEIN *F MTDROME03560 111/111 *F 53/68 CG3560 FBgn0030733 14B10 *F \------------- UCR6 P14927 UBIQUINOL-CYTOCHROME C REDUCTASE \---------- *F \------ \--------------- \------------ \---------- *F MTDROME17856 COMPLEX 14 KDA PROTEIN 111/111 *F 53/65 CG17856 FBgn0039576 98C2 *F MTDROME14482 UCRY O14957 UBIQUINOL-CYTOCHROME C REDUCTASE 56/57 *F 26/54 CG14482 FBgn0034245 54C9 *F COMPLEX 6.4 KDA PROTEIN *F MTDROME08764 UCRX Q9UDW1 UBIQUINOL-CYTOCHROME C REDUCTASE 62/55 *F 48/63 ox, FBgn0011227 49D2 7 alleles *F COMPLEX 7.2 KDA PROTEIN *F CG8764 *F MTDROME03731 UCR1 P31930 UBIQUINOL-CYTOCHROME C REDUCTASE 480/470 *F 54/71 CG3731 FBgn0038271 88D5 *F COMPLEX CORE PROTEIN I *F MTDROME04169 UCR2 P22695 UBIQUINOL-CYTOCHROME C REDUCTASE 453/440 *F 32/51 CG4169 FBgn0036642 73A4 *F COMPLEX CORE PROTEIN 2 *F UBIQUINOL-CYTOCHROME C REDUCTASE *F MTDROME07580 UCRQ O14949 COMPLEX UBIQUINONE-BINDING 81/89 *F 43/60 CG7580 FBgn0036728 74B2 *F PROTEIN QP-C *F UBIQUINOL-CYTOCHROME C REDUCTASE *F MTDROME07361 UCRI P47985 IRON-SULFUR SUBUNIT, 274/230 *F 51/61 RFeSP, FBgn0021906 22A3 k11704 *F MITOCHONDRIAL *F CG7361 M73 *F Ucp4A, *F MTDROME06492 323/340 *F 51/68 CG6492 FBgn0030872 16E1-2 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F UNCOUPLING PROTEIN 4, 323/337 *F 42/62 Ucp4B, *F MTDROME18340 UCP4 O95847 MITOCHONDRIAL 323/222 *F 26/38 CG18340 FBgn0031758 26A1 *F \------------- \---------- *F \------ \--------------- \------------ \---------- *F Ucp4C, *F MTDROME09064 323/335 *F 36/54 CG9064 FBgn0031757 26A1 *F POR1 *F porin, *F MTDROME06647 POR2) P21796 VOLTAGE-DEPENDENT 282/282 *F 58/76 CG6647 FBgn0004363 32A5 k05123 *F \------------- (P45880) ANION-SELECTIVE CHANNEL PROTEINS \---------- *F \------ \--------------- \------------ \---------- k08405 *F MTDROME17137 ( (Q9Y277) 282/293 *F 34/59 CG17137 FBgn0032308 32A5 \----------------------------- *F POR3) # *U FBrf0161091 *a Ryder *b E.J. *t 2003.6.17 *T personal communication to FlyBase *u *F From ejr34@mole.bio.cam.ac.uk Tue Jun 17 15:01:33 2003 *F To: ag24@gen.cam.ac.uk,r.drysdale@gen.cam.ac.uk *F Subject: deletions *F *F Please find attached 2 files. One contains all of the virtual deletions *F possible with drosdel v1.0 elements between 1bp and 1Mb (plus 2 others, *F one of which is 1.02Mb, and one which was made subsequently). *F The other file contains all of the deletions that have been made by the *F consortium (although not necessarily available to the public atm). *F ===================================== *F Dr. Ed Ryder *F Department of Genetics, University of Cambridge, *F Downing Street, Cambridge, CB2 3EH *F Tel: +44 (0)1223 765928. *F Email: e.ryder@gen.cam.ac.uk *F http://www.drosdel.org.uk *F ===================================== *F ED_no name2 loc2 band2 name1 loc1 band1 chr gap made *F Df(2L)ED2809 5-HA-1191 67365 21A4 CB-0264-3 72671 21A4 2L 5306 0 *F Df(2L)ED5878 5-HA-1191 67365 21A4 CB-5775-3 161120 21B2 2L 93755 0 *F Df(2L)ED4 5-HA-1191 67365 21A4 CB-5541-3 161484 21B2 2L 94119 0 *F Df(2L)ED10 5-HA-1191 67365 21A4 CB-6339-3 221924 21B5 2L 154559 0 *F Df(2L)ED13 5-HA-1191 67365 21A4 CB-5283-3 249365 21B6 2L 182000 0 *F Df(2L)ED25 5-HA-1191 67365 21A4 CB-5609-3 292463 21B8 2L 225098 0 *F Df(2L)ED45 5-HA-1191 67365 21A4 CB-5353-3 589648 21D1 2L 522283 0 *F Df(2L)ED76 5-HA-1191 67365 21A4 CB-0901-3 852310 21D3 2L 784945 0 *F Df(2L)ED83 5-HA-1191 67365 21A4 CB-0647-3 854492 21D4 2L 787127 0 *F Df(2L)ED90 5-HA-1191 67365 21A4 CB-5483-3 1038635 21E2 2L 971270 0 *F Df(2L)ED9 UM-8116-3 114685 21B1 5-HA-1693 183037 21B3 2L 68352 0 *F Df(2L)ED24 UM-8116-3 114685 21B1 5-SZ-3580 285747 21B7 2L 171062 0 *F Df(2L)ED37 UM-8116-3 114685 21B1 5-SZ-3961 492113 21C7 2L 377428 0 *F Df(2L)ED44 UM-8116-3 114685 21B1 5-SZ-3017 492518 21C7 2L 377833 0 *F Df(2L)ED59 UM-8116-3 114685 21B1 5-HA-1003 706585 21D2 2L 591900 0 *F Df(2L)ED67 UM-8116-3 114685 21B1 5-HA-1332 828453 21D3 2L 713768 0 *F Df(2L)ED75 UM-8116-3 114685 21B1 5-HA-1013 852310 21D3 2L 737625 0 *F Df(2L)ED8 UM-8348-3 131577 21B1 5-HA-1693 183037 21B3 2L 51460 0 *F Df(2L)ED22 UM-8348-3 131577 21B1 5-SZ-3580 285747 21B7 2L 154170 0 *F Df(2L)ED35 UM-8348-3 131577 21B1 5-SZ-3961 492113 21C7 2L 360536 0 *F Df(2L)ED42 UM-8348-3 131577 21B1 5-SZ-3017 492518 21C7 2L 360941 0 *F Df(2L)ED56 UM-8348-3 131577 21B1 5-HA-1003 706585 21D2 2L 575008 0 *F Df(2L)ED64 UM-8348-3 131577 21B1 5-HA-1332 828453 21D3 2L 696876 0 *F Df(2L)ED72 UM-8348-3 131577 21B1 5-HA-1013 852310 21D3 2L 720733 0 *F Df(2L)ED929 5-HA-1273 142636 21B1 CB-5775-3 161120 21B2 2L 18484 0 *F Df(2L)ED5 5-HA-1273 142636 21B1 CB-5541-3 161484 21B2 2L 18848 0 *F Df(2L)ED12 5-HA-1273 142636 21B1 CB-6339-3 221924 21B5 2L 79288 0 *F Df(2L)ED15 5-HA-1273 142636 21B1 CB-5283-3 249365 21B6 2L 106729 0 *F Df(2L)ED27 5-HA-1273 142636 21B1 CB-5609-3 292463 21B8 2L 149827 0 *F Df(2L)ED47 5-HA-1273 142636 21B1 CB-5353-3 589648 21D1 2L 447012 0 *F Df(2L)ED78 5-HA-1273 142636 21B1 CB-0901-3 852310 21D3 2L 709674 0 *F Df(2L)ED85 5-HA-1273 142636 21B1 CB-0647-3 854492 21D4 2L 711856 0 *F Df(2L)ED92 5-HA-1273 142636 21B1 CB-5483-3 1038635 21E2 2L 895999 0 *F Df(2L)ED6 CB-0416-3 159063 21B2 5-HA-1693 183037 21B3 2L 23974 0 *F Df(2L)ED19 CB-0416-3 159063 21B2 5-SZ-3580 285747 21B7 2L 126684 0 *F Df(2L)ED31 CB-0416-3 159063 21B2 5-SZ-3961 492113 21C7 2L 333050 0 *F Df(2L)ED38 CB-0416-3 159063 21B2 5-SZ-3017 492518 21C7 2L 333455 0 *F Df(2L)ED52 CB-0416-3 159063 21B2 5-HA-1003 706585 21D2 2L 547522 0 *F Df(2L)ED60 CB-0416-3 159063 21B2 5-HA-1332 828453 21D3 2L 669390 0 *F Df(2L)ED68 CB-0416-3 159063 21B2 5-HA-1013 852310 21D3 2L 693247 0 *F Df(2L)ED7 CB-5662-3 160605 21B2 5-HA-1693 183037 21B3 2L 22432 0 *F Df(2L)ED21 CB-5662-3 160605 21B2 5-SZ-3580 285747 21B7 2L 125142 0 *F Df(2L)ED34 CB-5662-3 160605 21B2 5-SZ-3961 492113 21C7 2L 331508 0 *F Df(2L)ED41 CB-5662-3 160605 21B2 5-SZ-3017 492518 21C7 2L 331913 0 *F Df(2L)ED55 CB-5662-3 160605 21B2 5-HA-1003 706585 21D2 2L 545980 0 *F Df(2L)ED63 CB-5662-3 160605 21B2 5-HA-1332 828453 21D3 2L 667848 0 *F Df(2L)ED71 CB-5662-3 160605 21B2 5-HA-1013 852310 21D3 2L 691705 0 *F Df(2L)ED11 5-SZ-3548 207391 21B4 CB-6339-3 221924 21B5 2L 14533 0 *F Df(2L)ED14 5-SZ-3548 207391 21B4 CB-5283-3 249365 21B6 2L 41974 0 *F Df(2L)ED26 5-SZ-3548 207391 21B4 CB-5609-3 292463 21B8 2L 85072 0 *F Df(2L)ED46 5-SZ-3548 207391 21B4 CB-5353-3 589648 21D1 2L 382257 0 *F Df(2L)ED77 5-SZ-3548 207391 21B4 CB-0901-3 852310 21D3 2L 644919 0 *F Df(2L)ED84 5-SZ-3548 207391 21B4 CB-0647-3 854492 21D4 2L 647101 0 *F Df(2L)ED91 5-SZ-3548 207391 21B4 CB-5483-3 1038635 21E2 2L 831244 0 *F Df(2L)ED18 5-HA-1691 222149 21B5 CB-5283-3 249365 21B6 2L 27216 0 *F Df(2L)ED30 5-HA-1691 222149 21B5 CB-5609-3 292463 21B8 2L 70314 0 *F Df(2L)ED51 5-HA-1691 222149 21B5 CB-5353-3 589648 21D1 2L 367499 0 *F Df(2L)ED82 5-HA-1691 222149 21B5 CB-0901-3 852310 21D3 2L 630161 0 *F Df(2L)ED89 5-HA-1691 222149 21B5 CB-0647-3 854492 21D4 2L 632343 0 *F Df(2L)ED96 5-HA-1691 222149 21B5 CB-5483-3 1038635 21E2 2L 816486 0 *F Df(2L)ED23 UM-8357-3 222377 21B5 5-SZ-3580 285747 21B7 2L 63370 0 *F Df(2L)ED36 UM-8357-3 222377 21B5 5-SZ-3961 492113 21C7 2L 269736 0 *F Df(2L)ED43 UM-8357-3 222377 21B5 5-SZ-3017 492518 21C7 2L 270141 0 *F Df(2L)ED58 UM-8357-3 222377 21B5 5-HA-1003 706585 21D2 2L 484208 0 *F Df(2L)ED66 UM-8357-3 222377 21B5 5-HA-1332 828453 21D3 2L 606076 0 *F Df(2L)ED74 UM-8357-3 222377 21B5 5-HA-1013 852310 21D3 2L 629933 0 *F Df(2L)ED20 UM-8106-3 225366 21B5 5-SZ-3580 285747 21B7 2L 60381 0 *F Df(2L)ED32 UM-8106-3 225366 21B5 5-SZ-3961 492113 21C7 2L 266747 0 *F Df(2L)ED39 UM-8106-3 225366 21B5 5-SZ-3017 492518 21C7 2L 267152 0 *F Df(2L)ED53 UM-8106-3 225366 21B5 5-HA-1003 706585 21D2 2L 481219 0 *F Df(2L)ED61 UM-8106-3 225366 21B5 5-HA-1332 828453 21D3 2L 603087 0 *F Df(2L)ED69 UM-8106-3 225366 21B5 5-HA-1013 852310 21D3 2L 626944 0 *F Df(2L)ED17 5-HA-1614 249337 21B6 CB-5283-3 249365 21B6 2L 28 0 *F Df(2L)ED29 5-HA-1614 249337 21B6 CB-5609-3 292463 21B8 2L 43126 0 *F Df(2L)ED50 5-HA-1614 249337 21B6 CB-5353-3 589648 21D1 2L 340311 0 *F Df(2L)ED81 5-HA-1614 249337 21B6 CB-0901-3 852310 21D3 2L 602973 0 *F Df(2L)ED88 5-HA-1614 249337 21B6 CB-0647-3 854492 21D4 2L 605155 0 *F Df(2L)ED95 5-HA-1614 249337 21B6 CB-5483-3 1038635 21E2 2L 789298 0 *F Df(2L)ED16 5-SZ-3153 249358 21B6 CB-5283-3 249365 21B6 2L 7 0 *F Df(2L)ED28 5-SZ-3153 249358 21B6 CB-5609-3 292463 21B8 2L 43105 0 *F Df(2L)ED48 5-SZ-3153 249358 21B6 CB-5353-3 589648 21D1 2L 340290 0 *F Df(2L)ED79 5-SZ-3153 249358 21B6 CB-0901-3 852310 21D3 2L 602952 0 *F Df(2L)ED86 5-SZ-3153 249358 21B6 CB-0647-3 854492 21D4 2L 605134 0 *F Df(2L)ED93 5-SZ-3153 249358 21B6 CB-5483-3 1038635 21E2 2L 789277 0 *F Df(2L)ED33 CB-0312-3 482538 21C7 5-SZ-3961 492113 21C7 2L 9575 0 *F Df(2L)ED40 CB-0312-3 482538 21C7 5-SZ-3017 492518 21C7 2L 9980 0 *F Df(2L)ED54 CB-0312-3 482538 21C7 5-HA-1003 706585 21D2 2L 224047 0 *F Df(2L)ED62 CB-0312-3 482538 21C7 5-HA-1332 828453 21D3 2L 345915 0 *F Df(2L)ED70 CB-0312-3 482538 21C7 5-HA-1013 852310 21D3 2L 369772 0 *F Df(2L)ED103 CB-0312-3 482538 21C7 5-HA-1599 1422194 22A1 2L 939656 0 *F Df(2L)ED57 CB-5083-3 512705 21C8 5-HA-1003 706585 21D2 2L 193880 0 *F Df(2L)ED65 CB-5083-3 512705 21C8 5-HA-1332 828453 21D3 2L 315748 0 *F Df(2L)ED73 CB-5083-3 512705 21C8 5-HA-1013 852310 21D3 2L 339605 0 *F Df(2L)ED107 CB-5083-3 512705 21C8 5-HA-1599 1422194 22A1 2L 909489 0 *F Df(2L)ED49 5-SZ-3596 569760 21D1 CB-5353-3 589648 21D1 2L 19888 0 *F Df(2L)ED80 5-SZ-3596 569760 21D1 CB-0901-3 852310 21D3 2L 282550 0 *F Df(2L)ED87 5-SZ-3596 569760 21D1 CB-0647-3 854492 21D4 2L 284732 0 *F Df(2L)ED94 5-SZ-3596 569760 21D1 CB-5483-3 1038635 21E2 2L 468875 0 *F Df(2L)ED105 CB-0616-3 854519 21D4 5-HA-1599 1422194 22A1 2L 567675 0 *F Df(2L)ED106 CB-6427-3 854604 21D4 5-HA-1599 1422194 22A1 2L 567590 0 *F Df(2L)ED108 CB-5389-3 1120800 21F1 5-HA-1599 1422194 22A1 2L 301394 0 *F Df(2L)ED104 CB-5673-3 1120856 21F1 5-HA-1599 1422194 22A1 2L 301338 0 *F Df(2L)ED111 5-SZ-4004 1729433 22B1 CB-0776-3 2171402 22D1 2L 441969 0 *F Df(2L)ED114 5-SZ-4004 1729433 22B1 CB-5692-3 2189688 22D3 2L 460255 0 *F Df(2L)ED118 5-SZ-4004 1729433 22B1 CB-0509-3 2214602 22D4 2L 485169 0 *F Df(2L)ED122 5-SZ-4004 1729433 22B1 CB-0243-3 2214632 22D4 2L 485199 0 *F Df(2L)ED126 5-SZ-4004 1729433 22B1 CB-0425-3 2214658 22D4 2L 485225 0 *F Df(2L)ED110 5-SZ-3989 1739130 22B2 CB-0776-3 2171402 22D1 2L 432272 0 *F Df(2L)ED113 5-SZ-3989 1739130 22B2 CB-5692-3 2189688 22D3 2L 450558 0 *F Df(2L)ED117 5-SZ-3989 1739130 22B2 CB-0509-3 2214602 22D4 2L 475472 0 *F Df(2L)ED121 5-SZ-3989 1739130 22B2 CB-0243-3 2214632 22D4 2L 475502 0 *F Df(2L)ED125 5-SZ-3989 1739130 22B2 CB-0425-3 2214658 22D4 2L 475528 0 *F Df(2L)ED109 5-SZ-3542 1987595 22B8 CB-0776-3 2171402 22D1 2L 183807 0 *F Df(2L)ED112 5-SZ-3542 1987595 22B8 CB-5692-3 2189688 22D3 2L 202093 0 *F Df(2L)ED115 5-SZ-3542 1987595 22B8 CB-0509-3 2214602 22D4 2L 227007 0 *F Df(2L)ED119 5-SZ-3542 1987595 22B8 CB-0243-3 2214632 22D4 2L 227037 0 *F Df(2L)ED123 5-SZ-3542 1987595 22B8 CB-0425-3 2214658 22D4 2L 227063 0 *F Df(2L)ED141 5-SZ-3542 1987595 22B8 CB-0891-3 2745701 23A3 2L 758106 0 *F Df(2L)ED160 5-SZ-3542 1987595 22B8 CB-0516-3 2745855 23A3 2L 758260 0 *F Df(2L)ED181 5-SZ-3542 1987595 22B8 CB-0916-3 2902276 23C1 2L 914681 0 *F Df(2L)ED130 CB-5150-3 2123449 22C3 5-HA-1055 2743650 23A3 2L 620201 0 *F Df(2L)ED137 CB-5150-3 2123449 22C3 5-HA-1056 2745694 23A3 2L 622245 0 *F Df(2L)ED148 CB-5150-3 2123449 22C3 5-HA-1642 2745701 23A3 2L 622252 0 *F Df(2L)ED156 CB-5150-3 2123449 22C3 5-HA-1873 2745793 23A3 2L 622344 0 *F Df(2L)ED168 CB-5150-3 2123449 22C3 5-SZ-3215 2866396 23B6 2L 742947 0 *F Df(2L)ED176 CB-5150-3 2123449 22C3 5-HA-1220 2901526 23C1 2L 778077 0 *F Df(2L)ED203 CB-5150-3 2123449 22C3 5-HA-1695 3048286 23D1 2L 924837 0 *F Df(2L)ED213 CB-5150-3 2123449 22C3 5-HA-1917 3048415 23D1 2L 924966 0 *F Df(2L)ED133 CB-0724-3 2123457 22C3 5-HA-1055 2743650 23A3 2L 620193 0 *F Df(2L)ED140 CB-0724-3 2123457 22C3 5-HA-1056 2745694 23A3 2L 622237 0 *F Df(2L)ED151 CB-0724-3 2123457 22C3 5-HA-1642 2745701 23A3 2L 622244 0 *F Df(2L)ED159 CB-0724-3 2123457 22C3 5-HA-1873 2745793 23A3 2L 622336 0 *F Df(2L)ED171 CB-0724-3 2123457 22C3 5-SZ-3215 2866396 23B6 2L 742939 0 *F Df(2L)ED180 CB-0724-3 2123457 22C3 5-HA-1220 2901526 23C1 2L 778069 0 *F Df(2L)ED4243 CB-0724-3 2123457 22C3 5-HA-1695 3048286 23D1 2L 924829 0 *F Df(2L)ED4557 CB-0724-3 2123457 22C3 5-HA-1917 3048415 23D1 2L 924958 0 *F Df(2L)ED128 CB-5125-3 2171359 22D1 5-HA-1055 2743650 23A3 2L 572291 0 *F Df(2L)ED135 CB-5125-3 2171359 22D1 5-HA-1056 2745694 23A3 2L 574335 0 *F Df(2L)ED146 CB-5125-3 2171359 22D1 5-HA-1642 2745701 23A3 2L 574342 0 *F Df(2L)ED154 CB-5125-3 2171359 22D1 5-HA-1873 2745793 23A3 2L 574434 0 *F Df(2L)ED166 CB-5125-3 2171359 22D1 5-SZ-3215 2866396 23B6 2L 695037 0 *F Df(2L)ED174 CB-5125-3 2171359 22D1 5-HA-1220 2901526 23C1 2L 730167 0 *F Df(2L)ED4106 CB-5125-3 2171359 22D1 5-HA-1695 3048286 23D1 2L 876927 0 *F Df(2L)ED4381 CB-5125-3 2171359 22D1 5-HA-1917 3048415 23D1 2L 877056 0 *F Df(2L)ED131 UM-8134-3 2189884 22D3 5-HA-1055 2743650 23A3 2L 553766 0 *F Df(2L)ED138 UM-8134-3 2189884 22D3 5-HA-1056 2745694 23A3 2L 555810 0 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CB-5064-3 19396685 18D13 X 463884 0 *F Df(1)ED7526 5-HA-1951 18932801 18B1 CB-5788-3 19414455 18E1 X 481654 0 *F Df(1)ED7532 5-HA-1951 18932801 18B1 CB-6514-3 19420374 18E1 X 487573 0 *F Df(1)ED7538 5-HA-1951 18932801 18B1 UM-8195-3 19444225 18E3 X 511424 0 *F Df(1)ED7550 5-HA-1951 18932801 18B1 CB-5666-3 19477069 18E5 X 544268 0 *F Df(1)ED7556 5-HA-1951 18932801 18B1 CB-5191-3 19477074 18E5 X 544273 0 *F Df(1)ED7562 5-HA-1951 18932801 18B1 CB-5637-3 19514517 18F2 X 581716 0 *F Df(1)ED7569 5-HA-1951 18932801 18B1 UM-8171-3 19514548 18F2 X 581747 0 *F Df(1)ED7584 5-HA-1951 18932801 18B1 CB-5436-3 19582446 18F4 X 649645 0 *F Df(1)ED7591 5-HA-1951 18932801 18B1 CB-5445-3 19617978 19A2 X 685177 0 *F Df(1)ED7629 5-HA-1951 18932801 18B1 CB-0927-3 19618116 19A2 X 685315 0 *F Df(1)ED7638 5-HA-1951 18932801 18B1 CB-5193-3 19861673 19B3 X 928872 0 *F Df(1)ED7432 5-HA-1964 18951547 18B2 CB-0521-3 19029733 18C1 X 78186 0 *F Df(1)ED7442 5-HA-1964 18951547 18B2 CB-5562-3 19053067 18C2 X 101520 0 *F Df(1)ED7457 5-HA-1964 18951547 18B2 CB-0310-3 19229249 18C8 X 277702 0 *F Df(1)ED7480 5-HA-1964 18951547 18B2 CB-0218-3 19349486 18D7 X 397939 0 *F Df(1)ED7487 5-HA-1964 18951547 18B2 CB-0219-3 19349486 18D7 X 397939 0 *F Df(1)ED7494 5-HA-1964 18951547 18B2 CB-0216-3 19349486 18D7 X 397939 0 *F Df(1)ED7501 5-HA-1964 18951547 18B2 CB-0217-3 19349486 18D7 X 397939 0 *F Df(1)ED7508 5-HA-1964 18951547 18B2 CB-5689-3 19368467 18D10 X 416920 0 *F Df(1)ED7514 5-HA-1964 18951547 18B2 CB-5064-3 19396685 18D13 X 445138 0 *F Df(1)ED7528 5-HA-1964 18951547 18B2 CB-5788-3 19414455 18E1 X 462908 0 *F Df(1)ED7534 5-HA-1964 18951547 18B2 CB-6514-3 19420374 18E1 X 468827 0 *F Df(1)ED7540 5-HA-1964 18951547 18B2 UM-8195-3 19444225 18E3 X 492678 0 *F Df(1)ED7552 5-HA-1964 18951547 18B2 CB-5666-3 19477069 18E5 X 525522 0 *F Df(1)ED7558 5-HA-1964 18951547 18B2 CB-5191-3 19477074 18E5 X 525527 0 *F Df(1)ED7564 5-HA-1964 18951547 18B2 CB-5637-3 19514517 18F2 X 562970 0 *F Df(1)ED7571 5-HA-1964 18951547 18B2 UM-8171-3 19514548 18F2 X 563001 0 *F Df(1)ED7586 5-HA-1964 18951547 18B2 CB-5436-3 19582446 18F4 X 630899 0 *F Df(1)ED7593 5-HA-1964 18951547 18B2 CB-5445-3 19617978 19A2 X 666431 0 *F Df(1)ED7631 5-HA-1964 18951547 18B2 CB-0927-3 19618116 19A2 X 666569 0 *F Df(1)ED7640 5-HA-1964 18951547 18B2 CB-5193-3 19861673 19B3 X 910126 0 *F Df(1)ED7445 CB-6351-3 19029155 18C1 5-SZ-3008 19054085 18C2 X 24930 0 *F Df(1)ED7461 CB-6351-3 19029155 18C1 5-SZ-3424 19241161 18C8 X 212006 0 *F Df(1)ED7466 CB-6351-3 19029155 18C1 5-SZ-3425 19241161 18C8 X 212006 0 *F Df(1)ED7471 CB-6351-3 19029155 18C1 5-SZ-3360 19241161 18C8 X 212006 0 *F Df(1)ED7519 CB-6351-3 19029155 18C1 5-SZ-3660 19398231 18D13 X 369076 0 *F Df(1)ED7577 CB-6351-3 19029155 18C1 5-HA-2039 19578283 18F3 X 549128 0 *F Df(1)ED7600 CB-6351-3 19029155 18C1 5-SZ-4105 19618066 19A2 X 588911 0 *F Df(1)ED7607 CB-6351-3 19029155 18C1 5-SZ-4104 19618066 19A2 X 588911 0 *F Df(1)ED7614 CB-6351-3 19029155 18C1 5-SZ-3422 19618087 19A2 X 588932 0 *F Df(1)ED7622 CB-6351-3 19029155 18C1 5-SZ-4100 19618087 19A2 X 588932 0 *F Df(1)ED7651 CB-6351-3 19029155 18C1 5-HA-1523 20003356 19C3 X 974201 0 *F Df(1)ED7444 CB-6037-3 19029550 18C1 5-SZ-3008 19054085 18C2 X 24535 0 *F Df(1)ED7459 CB-6037-3 19029550 18C1 5-SZ-3424 19241161 18C8 X 211611 0 *F Df(1)ED7464 CB-6037-3 19029550 18C1 5-SZ-3425 19241161 18C8 X 211611 0 *F Df(1)ED7469 CB-6037-3 19029550 18C1 5-SZ-3360 19241161 18C8 X 211611 0 *F Df(1)ED7515 CB-6037-3 19029550 18C1 5-SZ-3660 19398231 18D13 X 368681 0 *F Df(1)ED7573 CB-6037-3 19029550 18C1 5-HA-2039 19578283 18F3 X 548733 0 *F Df(1)ED7595 CB-6037-3 19029550 18C1 5-SZ-4105 19618066 19A2 X 588516 0 *F Df(1)ED7602 CB-6037-3 19029550 18C1 5-SZ-4104 19618066 19A2 X 588516 0 *F Df(1)ED7609 CB-6037-3 19029550 18C1 5-SZ-3422 19618087 19A2 X 588537 0 *F Df(1)ED7617 CB-6037-3 19029550 18C1 5-SZ-4100 19618087 19A2 X 588537 0 *F Df(1)ED7643 CB-6037-3 19029550 18C1 5-HA-1523 20003356 19C3 X 973806 0 *F Df(1)ED7452 5-HA-1900 19211286 18C7 CB-0310-3 19229249 18C8 X 17963 0 *F Df(1)ED7476 5-HA-1900 19211286 18C7 CB-0218-3 19349486 18D7 X 138200 0 *F Df(1)ED7483 5-HA-1900 19211286 18C7 CB-0219-3 19349486 18D7 X 138200 0 *F Df(1)ED7490 5-HA-1900 19211286 18C7 CB-0216-3 19349486 18D7 X 138200 0 *F Df(1)ED7497 5-HA-1900 19211286 18C7 CB-0217-3 19349486 18D7 X 138200 0 *F Df(1)ED7504 5-HA-1900 19211286 18C7 CB-5689-3 19368467 18D10 X 157181 0 *F Df(1)ED7510 5-HA-1900 19211286 18C7 CB-5064-3 19396685 18D13 X 185399 0 *F Df(1)ED7524 5-HA-1900 19211286 18C7 CB-5788-3 19414455 18E1 X 203169 0 *F Df(1)ED7530 5-HA-1900 19211286 18C7 CB-6514-3 19420374 18E1 X 209088 0 *F Df(1)ED7536 5-HA-1900 19211286 18C7 UM-8195-3 19444225 18E3 X 232939 0 *F Df(1)ED7548 5-HA-1900 19211286 18C7 CB-5666-3 19477069 18E5 X 265783 0 *F Df(1)ED7554 5-HA-1900 19211286 18C7 CB-5191-3 19477074 18E5 X 265788 0 *F Df(1)ED7560 5-HA-1900 19211286 18C7 CB-5637-3 19514517 18F2 X 303231 0 *F Df(1)ED7567 5-HA-1900 19211286 18C7 UM-8171-3 19514548 18F2 X 303262 0 *F Df(1)ED7582 5-HA-1900 19211286 18C7 CB-5436-3 19582446 18F4 X 371160 0 *F Df(1)ED7589 5-HA-1900 19211286 18C7 CB-5445-3 19617978 19A2 X 406692 0 *F Df(1)ED7627 5-HA-1900 19211286 18C7 CB-0927-3 19618116 19A2 X 406830 0 *F Df(1)ED7636 5-HA-1900 19211286 18C7 CB-5193-3 19861673 19B3 X 650387 0 *F Df(1)ED7657 5-HA-1900 19211286 18C7 CB-6266-3 20066485 19C5 X 855199 0 *F Df(1)ED7460 CB-0530-3 19229249 18C8 5-SZ-3424 19241161 18C8 X 11912 0 *F Df(1)ED7465 CB-0530-3 19229249 18C8 5-SZ-3425 19241161 18C8 X 11912 0 *F Df(1)ED7470 CB-0530-3 19229249 18C8 5-SZ-3360 19241161 18C8 X 11912 0 *F Df(1)ED7517 CB-0530-3 19229249 18C8 5-SZ-3660 19398231 18D13 X 168982 0 *F Df(1)ED7575 CB-0530-3 19229249 18C8 5-HA-2039 19578283 18F3 X 349034 0 *F Df(1)ED7597 CB-0530-3 19229249 18C8 5-SZ-4105 19618066 19A2 X 388817 0 *F Df(1)ED7604 CB-0530-3 19229249 18C8 5-SZ-4104 19618066 19A2 X 388817 0 *F Df(1)ED7611 CB-0530-3 19229249 18C8 5-SZ-3422 19618087 19A2 X 388838 0 *F Df(1)ED7619 CB-0530-3 19229249 18C8 5-SZ-4100 19618087 19A2 X 388838 0 *F Df(1)ED7645 CB-0530-3 19229249 18C8 5-HA-1523 20003356 19C3 X 774107 0 *F Df(1)ED7516 CB-0200-3 19241326 18C8 5-SZ-3660 19398231 18D13 X 156905 0 *F Df(1)ED7574 CB-0200-3 19241326 18C8 5-HA-2039 19578283 18F3 X 336957 0 *F Df(1)ED7596 CB-0200-3 19241326 18C8 5-SZ-4105 19618066 19A2 X 376740 0 *F Df(1)ED7603 CB-0200-3 19241326 18C8 5-SZ-4104 19618066 19A2 X 376740 0 *F Df(1)ED7610 CB-0200-3 19241326 18C8 5-SZ-3422 19618087 19A2 X 376761 0 *F Df(1)ED7618 CB-0200-3 19241326 18C8 5-SZ-4100 19618087 19A2 X 376761 0 *F Df(1)ED7644 CB-0200-3 19241326 18C8 5-HA-1523 20003356 19C3 X 762030 0 *F Df(1)ED7521 CB-6344-3 19362961 18D8 5-SZ-3660 19398231 18D13 X 35270 0 *F Df(1)ED7579 CB-6344-3 19362961 18D8 5-HA-2039 19578283 18F3 X 215322 0 *F Df(1)ED7601 CB-6344-3 19362961 18D8 5-SZ-4105 19618066 19A2 X 255105 0 *F Df(1)ED7608 CB-6344-3 19362961 18D8 5-SZ-4104 19618066 19A2 X 255105 0 *F Df(1)ED7615 CB-6344-3 19362961 18D8 5-SZ-3422 19618087 19A2 X 255126 0 *F Df(1)ED7623 CB-6344-3 19362961 18D8 5-SZ-4100 19618087 19A2 X 255126 0 *F Df(1)ED7652 CB-6344-3 19362961 18D8 5-HA-1523 20003356 19C3 X 640395 0 *F Df(1)ED7518 CB-5736-3 19368264 18D10 5-SZ-3660 19398231 18D13 X 29967 0 *F Df(1)ED7576 CB-5736-3 19368264 18D10 5-HA-2039 19578283 18F3 X 210019 0 *F Df(1)ED7598 CB-5736-3 19368264 18D10 5-SZ-4105 19618066 19A2 X 249802 0 *F Df(1)ED7605 CB-5736-3 19368264 18D10 5-SZ-4104 19618066 19A2 X 249802 0 *F Df(1)ED7612 CB-5736-3 19368264 18D10 5-SZ-3422 19618087 19A2 X 249823 0 *F Df(1)ED7620 CB-5736-3 19368264 18D10 5-SZ-4100 19618087 19A2 X 249823 0 *F Df(1)ED7648 CB-5736-3 19368264 18D10 5-HA-1523 20003356 19C3 X 635092 0 *F Df(1)ED7565 5-HA-1030 19481746 18F1 CB-5637-3 19514517 18F2 X 32771 0 *F Df(1)ED7572 5-HA-1030 19481746 18F1 UM-8171-3 19514548 18F2 X 32802 0 *F Df(1)ED7587 5-HA-1030 19481746 18F1 CB-5436-3 19582446 18F4 X 100700 0 *F Df(1)ED7594 5-HA-1030 19481746 18F1 CB-5445-3 19617978 19A2 X 136232 0 *F Df(1)ED7632 5-HA-1030 19481746 18F1 CB-0927-3 19618116 19A2 X 136370 0 *F Df(1)ED7641 5-HA-1030 19481746 18F1 CB-5193-3 19861673 19B3 X 379927 0 *F Df(1)ED7658 5-HA-1030 19481746 18F1 CB-6266-3 20066485 19C5 X 584739 0 *F Df(1)ED7599 CB-5613-3 19618040 19A2 5-SZ-4105 19618066 19A2 X 26 0 *F Df(1)ED7606 CB-5613-3 19618040 19A2 5-SZ-4104 19618066 19A2 X 26 0 *F Df(1)ED7613 CB-5613-3 19618040 19A2 5-SZ-3422 19618087 19A2 X 47 0 *F Df(1)ED7621 CB-5613-3 19618040 19A2 5-SZ-4100 19618087 19A2 X 47 0 *F Df(1)ED7649 CB-5613-3 19618040 19A2 5-HA-1523 20003356 19C3 X 385316 0 *F Df(1)ED7616 CB-6272-3 19618075 19A2 5-SZ-3422 19618087 19A2 X 12 0 *F Df(1)ED7624 CB-6272-3 19618075 19A2 5-SZ-4100 19618087 19A2 X 12 0 *F Df(1)ED7654 CB-6272-3 19618075 19A2 5-HA-1523 20003356 19C3 X 385281 0 *F Df(1)ED7646 CB-0640-3 19618087 19A2 5-HA-1523 20003356 19C3 X 385269 0 *F Df(1)ED7626 5-SZ-3656 19618115 19A2 CB-0927-3 19618116 19A2 X 1 0 *F Df(1)ED7635 5-SZ-3656 19618115 19A2 CB-5193-3 19861673 19B3 X 243558 0 *F Df(1)ED7656 5-SZ-3656 19618115 19A2 CB-6266-3 20066485 19C5 X 448370 0 *F Df(1)ED7653 CB-0259-3 19618219 19A2 5-HA-1523 20003356 19C3 X 385137 0 *F Df(1)ED7633 5-HA-1732 19618224 19A2 CB-5193-3 19861673 19B3 X 243449 0 *F Df(1)ED7655 5-HA-1732 19618224 19A2 CB-6266-3 20066485 19C5 X 448261 0 *F Df(1)ED7647 CB-0675-3 19618293 19A2 5-HA-1523 20003356 19C3 X 385063 0 *F Df(1)ED7642 CB-0019-3 19940156 19C1 5-HA-1523 20003356 19C3 X 63200 0 *F Df(1)ED7650 CB-0905-3 20003228 19C3 5-HA-1523 20003356 19C3 X 128 0 *F Df(1)ED7660 5-SZ-4066 20796045 19F1 CB-5138-3 20953620 19F3 X 157575 0 *F Df(1)ED7662 5-SZ-4066 20796045 19F1 CB-5663-3 20953709 19F3 X 157664 0 *F Df(1)ED7664 5-SZ-4066 20796045 19F1 CB-5128-3 21046418 19F6 X 250373 0 *F Df(1)ED7659 5-SZ-4110 20953132 19F3 CB-5138-3 20953620 19F3 X 488 0 *F Df(1)ED7661 5-SZ-4110 20953132 19F3 CB-5663-3 20953709 19F3 X 577 0 *F Df(1)ED7663 5-SZ-4110 20953132 19F3 CB-5128-3 21046418 19F6 X 93286 0 *F ============= *F ED_no name2 loc2 band2 name1 loc1 band1 chr gap made *F Df(2L)ED7666 CB-5641-3 2485502 22F3 5-HA-1233 2486690 22F3 2L 1188 1 *F Df(2L)ED165 CB-0939-3 2486662 22F3 5-SZ-3215 2866396 23B6 2L 379734 1 *F Df(2L)ED206 CB-0337-3 2866523 23B6 5-HA-1695 3048286 23D1 2L 181763 1 *F Df(2L)ED4651 CB-0337-3 2866523 23B6 5-HA-1143 3470876 23F6 2L 604353 1 *F Df(2L)ED270 CB-0544-3 5650844 25F2 5-HA-1420 5792411 25F5 2L 141567 1 *F Df(2L)ED284 CB-0544-3 5650844 25F2 5-HA-1725 5936177 26A3 2L 285333 1 *F Df(2L)ED695 CB-0733-3 9691454 30C5 5-HA-1142 9910601 30E4 2L 219147 1 *F Df(2L)ED700 CB-0374-3 9889753 30E1 5-HA-1142 9910601 30E4 2L 20848 1 *F Df(2L)ED746 5-HA-1552 10499182 31F4 CB-0279-3 10725113 32A4 2L 225931 1 *F Df(2L)ED775 5-SZ-3619 11998881 33B8 CB-0356-3 12963898 34A3 2L 965017 1 *F Df(2L)ED800 5-HA-1150 14472727 35B2 CB-0185-3 15310773 35D1 2L 838046 1 *F Df(2L)ED3 5-HA-1150 14472727 35B2 CB-0183-3 15311845 35D1 2L 839118 1 *F Df(2L)ED1203 CB-5022-3 18595308 36F7 5-HA-1269 19136530 37C5 2L 541222 1 *F Df(2L)ED1226 CB-0762-3 18981481 37B9 5-HA-1039 19442139 37E3 2L 460658 1 *F Df(2L)ED1303 5-SZ-3616 19495693 37E5 CB-5090-3 20352127 38C6 2L 856434 1 *F Df(2L)ED1305 5-HA-1723 20063474 38B4 CB-5090-3 20352127 38C6 2L 288653 1 *F Df(2L)ED1315 5-HA-1723 20063474 38B4 CB-5075-3 20887261 38F5 2L 823787 1 *F Df(2L)ED1317 5-HA-1687 20608322 38D1 CB-5075-3 20887261 38F5 2L 278939 1 *F Df(2L)ED1455 5-HA-1213 21021870 39A1 CB-0697-3 21490798 39E6 2L 468928 1 *F Df(2L)ED1473 5-HA-1182 21220636 39B4 CB-5359-3 21661629 40A5 2L 440993 1 *F Df(2L)ED1466 5-HA-1496 21462437 39E3 CB-5359-3 21661629 40A5 2L 199192 1 *F Df(2R)ED1 CB-0140-3 12090452 53E10 5-HA-1118 12161047 53F9 2R 70595 1 *F Df(3L)ED201 CB-5349-3 104554 61B1 5-SZ-3091 328571 61C1 3L 224017 1 *F Df(3L)ED4191 CB-0396-3 524902 61C3 5-SZ-3468 1459566 62A2 3L 934664 1 *F Df(3L)ED4196 CB-5006-3 620212 61C7 5-SZ-3468 1459566 62A2 3L 839354 1 *F Df(3L)ED202 5-SZ-3251 719368 61C9 CB-5013-3 1317010 61F7 3L 597642 1 *F Df(3L)ED4238 5-SZ-3251 719368 61C9 CB-0748-3 1527560 62A5 3L 808192 1 *F Df(3L)ED207 5-SZ-3251 719368 61C9 CB-5091-3 1548737 62A6 3L 829369 1 *F Df(3L)ED208 CB-0230-3 3229777 63C1 5-HA-1116 3873777 63F5 3L 644000 1 *F Df(3L)ED4342 CB-0907-3 4258613 64B1 5-SZ-3243 4603313 64B13 3L 344700 1 *F Df(3L)ED210 CB-0753-3 4522175 64B11 5-SZ-3218 5314650 64D1 3L 792475 1 *F Df(3L)ED211 5-SZ-3253 6177664 65A9 CB-0548-3 6512288 65B4 3L 334624 1 *F Df(3L)ED212 CB-0040-3 6178182 65A9 5-HA-1091 6924270 65D5 3L 746088 1 *F Df(3L)ED4408 CB-6289-3 7938638 66A22 5-HA-1500 8258284 66C5 3L 319646 1 *F Df(3L)ED4421 5-HA-1672 8704036 66D14 CB-5112-3 9342785 67B1 3L 638749 1 *F Df(3L)ED4470 5-SZ-3283 11054521 68A6 CB-5550-3 11790716 68E1 3L 736195 1 *F Df(3L)ED4475 CB-5549-3 11544572 68C13 5-SZ-3455 12366148 69B4 3L 821576 1 *F Df(3L)ED4483 5-SZ-3469 12234767 69A4 CB-6051-3 12650761 69D3 3L 415994 1 *F Df(3L)ED215 5-HA-1076 12375100 69B5 CB-5040-3 12461845 69C4 3L 86745 1 *F Df(3L)ED4486 CB-6193-3 12471966 69C4 5-SZ-3267 12990032 69F6 3L 518066 1 *F Df(3L)ED4543 5-SZ-3479 13884105 70C6 CB-6268-3 14706920 70F4 3L 822815 1 *F Df(3L)ED217 CB-0754-3 14706950 70F4 5-SZ-3089 15537975 71E1 3L 831025 1 *F Df(3L)ED218 CB-5025-3 14962947 71B1 5-SZ-3089 15537975 71E1 3L 575028 1 *F Df(3L)ED219 CB-0072-3 15481449 71E1 5-HA-1128 16360556 72F1 3L 879107 1 *F Df(3L)ED220 CB-0003-3 16036363 72D4 5-HA-1128 16360556 72F1 3L 324193 1 *F Df(3L)ED4606 CB-0003-3 16036363 72D4 5-SZ-3234 16729003 73C4 3L 692640 1 *F Df(3L)ED223 CB-5452-3 16400704 73A1 5-SZ-3248 16839757 73D5 3L 439053 1 *F Df(3L)ED224 5-HA-1089 17918082 75B2 CB-0578-3 18347398 75C6 3L 429316 1 *F Df(3L)ED225 CB-5465-3 18135024 75C1 5-HA-1189 18570216 75D4 3L 435192 1 *F Df(3L)ED4782 5-SZ-3490 18944773 75F2 UM-8378-3 19119581 76A1 3L 174808 1 *F Df(3L)ED4786 CB-6325-3 19049830 75F7 5-SZ-3669 19244541 76A5 3L 194711 1 *F Df(3L)ED4789 CB-5135-3 19119585 76A1 5-SZ-3669 19244541 76A5 3L 124956 1 *F Df(3L)ED4799 CB-5135-3 19119585 76A1 5-SZ-3487 19431051 76B3 3L 311466 1 *F Df(3L)ED228 CB-5135-3 19119585 76A1 5-SZ-3185 19790347 76D2 3L 670762 1 *F Df(3L)ED229 CB-5135-3 19119585 76A1 5-HA-1123 19921249 76E1 3L 801664 1 *F Df(3L)ED4858 5-SZ-4114 19813911 76D3 UM-8189-3 20320358 77C1 3L 506447 1 *F Df(3L)ED230 CB-0142-3 22051929 79C2 5-HA-1247 22751649 80A4 3L 699720 1 *F Df(3L)ED231 5-SZ-3080 22789094 80B2 CB-5309-3 22862798 80C1 3L 73704 1 *F Df(3R)ED5092 5-SZ-3048 107091 82A3 CB-5306-3 912804 82E8 3R 805713 1 *F Df(3R)ED5066 5-HA-1188 475606 82D1 CB-5152-3 778401 82E4 3R 302795 1 *F Df(3R)ED5095 5-HA-1188 475606 82D1 CB-5306-3 912804 82E8 3R 437198 1 *F Df(3R)ED7665 5-HA-3039 2916250 84B6 CB-0050-3 3919820 84E10 3R 1003570 1 *F Df(3R)ED2 CB-0160-3 14224969 91A5 5-HA-1087 14922510 91F1 3R 697541 1 *F Df(4)ED6364 CB-0038-3 79870 102A4 5-HA-1572 213735 102B1 4 133865 1 *F Df(4)ED6366 CB-5204-3 90336 102A4 5-HA-1572 213735 102B1 4 123399 1 *F Df(4)ED6369 CB-5204-3 90336 102A4 5-SZ-3463 473880 102C1 4 383544 1 *F Df(1)ED404 5-SZ-3683 692379 1D2 CB-5340-3 892882 1E3 X 200503 1 *F Df(1)ED409 5-HA-1028 1798999 2C7 CB-0780-3 2074461 2F5 X 275462 1 *F Df(1)ED411 CB-0004-3 2226572 3A3 5-SZ-3093 2399458 3A8 X 172886 1 *F Df(1)ED418 5-SZ-3675 5642065 5C7 CB-5199-3 6019777 5E4 X 377712 1 *F Df(1)ED429 CB-0612-3 10287124 9D3 5-HA-1157 10325691 9D3 X 38567 1 *F Df(1)ED447 5-HA-1134 18237900 17C1 CB-0402-3 18594695 17F1 X 356795 1 # *U FBrf0161459 *a Mi *b H. *c P. *d Thomas *e S.E. *f Lewis *g M. *h Ashburner *t 2003.9.9 *T personal communication to FlyBase *u *F The following GO term predictions are from Huaiyu Mi and Paul Thomas *F (Celera at Foster City, CA) using the PANTHER protocol (Thomas et al. *F 2003, Genome Res. 13:2129). This is a repeat of the work described in *F Mi et al. 2003, Genome Res. 13:2118, but performed on the Release 3.1 *F sequence. *F *F See also: http://panther.celera.com *F *F Huaiyu Mi and Paul Thomas provided FlyBase with a file (dated June 26 *F 2003) of all PANTHER predictions of GO terms for Drosophila gene *F products. The PANTHER confidence scores for the GO predictions are *F rated as follows: *F *F <-85 closely related *F between -50 to -85 related *F between -35 to -50 distantly related *F between -20 to -35 no rating. Likely to be not related. *F *F For incorporation of these predicted GO terms into FlyBase, the *F original file was processed as follows: *F *F 1. Lines where the NLL-NULL score was greater than -85 (Mi et al. *F 2003 Genome Research 13:2118) were removed. *F *F 2. All remaining lines with molecular_function unknown ; *F GO:0005554, biological_process unknown ; GO:0000004 and *F cellular_component unknown ; GO:0008372 were removed. *F *F 3. The following parsing errors were corrected. *F *F i. cell proliferation ; GO:0030154 cell differentiation ; GO:0008283 *F was edited to: cell proliferation ; GO:0008283 *F *F ii. protein targeting ; GO:0008104 protein localization ; GO:0006605 *F was edited to: protein targeting ; GO:0006605 *F *F iii. intracellular protein transport ; GO:0006605 was edited to: *F protein targeting ; GO:0006605. This was the only case of the same GO *F string being attached to more than one GO identifier. *F *F 4. 74 duplicate lines were removed. *F *F 5. For all FBgn's with no current GO annotation, PANTHER GO term *F predictions were automatically added into FlyBase under this personal *F communication, supported by the IEA (inferred from electronic *F annotation) evidence code. *F *F 6. For all FBgn's with current GO annotation, PANTHER GO term predictions *F were manually checked for conflicts with exisiting GO terms. Excluding *F redundant PANTHER predictions (where the same or a higher-level GO term *F already exists in FlyBase), and very broad PANTHER predictions *F (including development ; GO:0007275 and metabolism ; GO:0008152), *F non-conflicting PANTHER predictions were added into FlyBase under this *F personal communication, supported by the IEA evidence code. *F *F Predicted GO terms that were not applicable to Drosophila were not added *F into FlyBase for any genes. # *U FBrf0161497 *a Robertson *b H. *t 2003.6.22 *T personal communication to FlyBase *u FlyBase error report for CG13788 on Sat Jun 21 18:56:30 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sat, 21 Jun 2003 18:56:30 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG13788 on Sat Jun 21 18:56:30 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG13788 *F Release: 3 *F Gene annotation error *F Gene CG13788 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Gr28bA *F ATGATCCGCTGCGGATTGGACATTTTTCGCGGCTGCCGAGGACGATTCCGCTACTGGTTGAGTGCCCGGGATTGCTATG *F ATTCCATTTCGTTGATGGTGGCCATTGCCTTTGCTTTGGGCATCACACCGTTTCTGGTGAGGCGAAACGCGCTGGGGGA *F AAATTCCTTAGAACAGTCGTGGTATGGATTCCTAAATGCCATTTTTCGCTGGCTACTGCTCGCCTATTGCTACAGCTAC *F ATTAACCTGAGAAACGAGAGCCTGATTGGCTATTTCATGAGGAACCATGTGTCACAGATAAGCACACGAGTCCATGACG *F TCGGTGGCATTATAGCGGCCGTGTTCACCTTTATATTGCCGCTACTTCTGCGAAAATACTTCCTGAAATCTGTCAAGAA *F TATGGTGCAGGTTGATACACAACTAGAACGTTTGCGAAGTCCTGTCAATTTCAATACGGTTGTAGGCCAGGTGGTCCTG *F GTCATATTGGCGGTAGTTCTCCTGGATACCGTCCTTCTGACAACTGGCTTAGTTTGCCTGGCCAAAATGGAGGTGTACG *F CTTCGTGGCAACTCACCTTCATTTTTGTCTACGAGCTCCTGGCCATTTCCATCACCATCTGCATGTTTTGTCTGATGAC *F CAGAACGGTTCAGCGCCGAATCACCTGCCTTCACAAGGTGTTGAAGAACCTAGCCCATCAATGGGACACCCGAAGCCTC *F AAGGCAGTGAATCAAAAACAGCGCTCTCTACAATGTCTCGATTCATTTTCCATGTACACCATTGTAACCAAGGATCCTG *F CGGAGATTATACAGGAGTCCATGGAGATACATCATCTCATTTGCGAGGCAGCTGCCACGGCTAACAAATATTTTACCTA *F CCAATTGCTGACCATTATATCCATAGCATTTCTGATCATCGTTTTCGATGCATACTATGTTCTGGAGACGCTACTGGGA *F AAATCGAAGCGCGAAAGCAAATTCAAAACTGTGGAATTTGTGACATTTTTCTCGTGTCAAATGATTTTGTATCTGATCG *F CCATAATTTCCATTGTCGAGGGAAGTAATCGAGCCATCAAAAAGAGCGAGAAAACTGGAGGCATAGTGCACTCCCTACT *F CAATAAAACCAAAAGTGCTGAGGTCAAGGAGAAACTGCAGCAATTCTCCATGCAGTTGATGCATCTGAAAATTAATTTT *F ACTGCAGCTGGTCTGTTCAACATCGACCGCACATTGTATTTCACGATCAGCGGGGCCTTGACCACTTATCTCATCATCT *F TGCTGCAGTTCACATCCAATTCCCCGAACAATGGTTATGGGAATGGCAGCTCTTGCTGTGAGACCTTCAATAATATGAC *F GAATCATACGCTTTAG *F >Gr28bB *F ATGTCCGCCCTGCGTCGGGTGCGCAAATACTTTATATCCTCTCAGGTCTACGAGGCACTGCGTCCACTATTCTTCCTAA *F CGTTTCTATACGGACTAACGCCATTTCATGTGGTCAGACGAAAGATGGGGGAATCCTACTTGAAGATGTCCTGTTTCGG *F TGTCTTCAACATCTTCATTTACATCTGCCTCTGCGGATTCTGCTACATTTCATCCCTGAGGCAGGGAGAATCCATTGTG *F GGATATTTCTTTCGCACCGAAATCTCCACAATCGGCGATCGTTTGCAGATCTTTAATGGTTTGATAGCTGGTGCTGTGA *F TTTATACGTCGGCAATCCTAAAACGCTGCAAGCTTTTGGGCACACTGACCATTCTACACAGCTTGGATACGAACTTCTC *F GAACATTGGAGTACGTGTGAAATATTCGAGAATATTCCGGTACTCGCTACTGGTGCTGATCTTCAAGCTACTGATCCTG *F GGTGTTTATTTCGTTGGAGTTTTCCGGCTGCTCGTCTCCCTGGATGTCACACCATCGTTCTGCGTTTGTATGACATTCT *F TTCTGCAACATTCGGTTGTCTCCATAGCGATTTGTTTGTTCTGCGTGATTGCCTTCAGTTTCGAGCGGCGCCTCAGTAT *F CATCAATCAGGTGTTGAAGAACCTAGCCCATCAATGGGACACCCGAAGCCTCAAGGCAGTGAATCAAAAACAGCGCTCT *F CTACAATGTCTCGATTCATTTTCCATGTACACCATTGTAACCAAGGATCCTGCGGAGATTATACAGGAGTCCATGGAGA *F TACATCATCTCATTTGCGAGGCAGCTGCCACGGCTAACAAATATTTTACCTACCAATTGCTGACCATTATATCCATAGC *F ATTTCTGATCATCGTTTTCGATGCATACTATGTTCTGGAGACGCTACTGGGAAAATCGAAGCGCGAAAGCAAATTCAAA *F ACTGTGGAATTTGTGACATTTTTCTCGTGTCAAATGATTTTGTATCTGATCGCCATAATTTCCATTGTCGAGGGAAGTA *F ATCGAGCCATCAAAAAGAGCGAGAAAACTGGAGGCATAGTGCACTCCCTACTCAATAAAACCAAAAGTGCTGAGGTCAA *F GGAGAAACTGCAGCAATTCTCCATGCAGTTGATGCATCTGAAAATTAATTTTACTGCAGCTGGTCTGTTCAACATCGAC *F CGCACATTGTATTTCACGATCAGCGGGGCCTTGACCACTTATCTCATCATCTTGCTGCAGTTCACATCCAATTCCCCGA *F ACAATGGTTATGGGAATGGCAGCTCTTGCTGTGAGACCTTCAATAATATGACGAATCATACGCTTTAG *F >Gr28bC *F ATGGACATTGAAATGGCCAAGGAGCCGGTGAATCCAACGGATACTCCGGATATAGAAGTGACTCCCGGTCTATGCCAGC *F CCTTGCGTCGTAGATTTCGGCGATTTGTTACCGCCAAACAGCTATACGAGTGCCTGCGCCCGGTGTTCCATGTGACCTA *F CATCCACGGACTCACCTCCTTCTACATTAGTTGCGATACTAAAACCGGAAAGAAAGCCATCAAGAAAACCATTTTCGGC *F TACATCAATGGAATCATGCACATCGCCATGTTTGTCTTTGCCTATAGCCTTACGATTTATAACAATTGCGAGTCGGTGG *F CTAGTTACTTCTTTCGATCTCGCATTACCTATTTCGGGGATTTGATGCAGATAGTGAGTGGATTCATTGGAGTTACTGT *F CATCTACCTGACTGCCTTTGTCCCAAACCATCGATTGGAGCGTTGCCTTCAGAAGTTTCACACCATGGACGTGCAACTC *F CAGACGGTGGGAGTGAAGATCATGTACAGCAAGGTGCTGCGATTTAGCTACATGGTCCTGATCTCCATGTTCCTTGTAA *F ACGTACTCTTTACCGGCGGCACCTTTTCGGTTCTCTATTCCTCGGAAGTGGCGCCCACCATGGCCCTGCACTTCACCTT *F CCTCATCCAGCACACGGTCATCGCCATTGCCATTGCGCTCTTCAGTTGCTTCACATATCTGGTGGAGATGCGACTGGTG *F ATGGTCAATAAGGTGTTGAAGAACCTAGCCCATCAATGGGACACCCGAAGCCTCAAGGCAGTGAATCAAAAACAGCGCT *F CTCTACAATGTCTCGATTCATTTTCCATGTACACCATTGTAACCAAGGATCCTGCGGAGATTATACAGGAGTCCATGGA *F GATACATCATCTCATTTGCGAGGCAGCTGCCACGGCTAACAAATATTTTACCTACCAATTGCTGACCATTATATCCATA *F GCATTTCTGATCATCGTTTTCGATGCATACTATGTTCTGGAGACGCTACTGGGAAAATCGAAGCGCGAAAGCAAATTCA *F AAACTGTGGAATTTGTGACATTTTTCTCGTGTCAAATGATTTTGTATCTGATCGCCATAATTTCCATTGTCGAGGGAAG *F TAATCGAGCCATCAAAAAGAGCGAGAAAACTGGAGGCATAGTGCACTCCCTACTCAATAAAACCAAAAGTGCTGAGGTC *F AAGGAGAAACTGCAGCAATTCTCCATGCAGTTGATGCATCTGAAAATTAATTTTACTGCAGCTGGTCTGTTCAACATCG *F ACCGCACATTGTATTTCACGATCAGCGGGGCCTTGACCACTTATCTCATCATCTTGCTGCAGTTCACATCCAATTCCCC *F GAACAATGGTTATGGGAATGGCAGCTCTTGCTGTGAGACCTTCAATAATATGACGAATCATACGCTTTAG *F >Gr28bD *F ATGTCATTTTACTTTTGCGAAATATTCAAACCTCGAGATGCTTTCGGAGCCGAGCAAACCTTACTTTTGTACACCTATC *F TACTCGGTTTGACCCCTTTTCGTTTAAGGGGTCAGGCTGGTGAGAGGCAGTTTCATCTAAGTAAAATCGGCTATCTGAA *F TGCTTTTCTTCAGTTGAGTTTCTTCAGCTATTGCTTTTTGGCCGCTCTCATCGAACAGCAGAGTATTGTTGGCTATTTT *F TTCAAATCGGAAATTTCTCAAATGGGAGACTCACTGCAAAAATTCATCGGCATGACTGGAATGTCGATACTGTTCCTCT *F GCAGCAGCATTCGTGTTAGATTGCTGATCCATATATGGGATCGTATTTCCTATATAGATGATCGTTTTCTCAACTTGGG *F CGTATGCTTCAATTATCCCGCCATTATGCGATTGAGACTTTTACAGATCTTCCTCATTAATGGCGTGCAATTGGGCTAT *F TTGATAAGTTCCAACTGGATGTTGTTGGGTAATGATGTGCGACCCATTTACACAGCAATTGTGGCGTTCTACGTGCCCC *F AAATATTTCTACTAAGTATTGTTATGCTGTTTAACGCTACATTACATCGTCTCTGGCAACATTTCACTGTACTGAATCA *F GGTGTTGAAGAACCTAGCCCATCAATGGGACACCCGAAGCCTCAAGGCAGTGAATCAAAAACAGCGCTCTCTACAATGT *F CTCGATTCATTTTCCATGTACACCATTGTAACCAAGGATCCTGCGGAGATTATACAGGAGTCCATGGAGATACATCATC *F TCATTTGCGAGGCAGCTGCCACGGCTAACAAATATTTTACCTACCAATTGCTGACCATTATATCCATAGCATTTCTGAT *F CATCGTTTTCGATGCATACTATGTTCTGGAGACGCTACTGGGAAAATCGAAGCGCGAAAGCAAATTCAAAACTGTGGAA *F TTTGTGACATTTTTCTCGTGTCAAATGATTTTGTATCTGATCGCCATAATTTCCATTGTCGAGGGAAGTAATCGAGCCA *F TCAAAAAGAGCGAGAAAACTGGAGGCATAGTGCACTCCCTACTCAATAAAACCAAAAGTGCTGAGGTCAAGGAGAAACT *F GCAGCAATTCTCCATGCAGTTGATGCATCTGAAAATTAATTTTACTGCAGCTGGTCTGTTCAACATCGACCGCACATTG *F TATTTCACGATCAGCGGGGCCTTGACCACTTATCTCATCATCTTGCTGCAGTTCACATCCAATTCCCCGAACAATGGTT *F ATGGGAATGGCAGCTCTTGCTGTGAGACCTTCAATAATATGACGAATCATACGCTTTAG *F >Gr28bE *F ATGTGGCTCCTTAGGCGATCGGTTGGAAAATCGGGCAACCGACCACACGACGTATACACCTGCTATCGATTGACTATAT *F TCATGGCACTTTGTCTCGGAATTGTGCCTTACTACGTATCCATATCTTCGGAAGGCAGAGGAAAACTAACATCCTCGTA *F CATCGGCTACATCAACATCATCATACGAATGGCCATATATATGGTCAACTCATTCTACGGCGCCGTCAATCGGGATACG *F TTGATGTCCAACTTTTTCCTTACAGACATATCCAATGTGATAGATGCTTTGCAAAAGATCAATGGAATGCTGGGAATAT *F TTGCCATATTACTTATATCGCTATTGAACCGAAAAGAATTGCTAAAACTGCTGGCCACATTCGATAGACTTGAGACGGA *F GGCGTTTCCACGGGTGGGCGTGGCAATGCACCAGGTTGCAGCTAATAAGAAAATGAATCGATTGGTTATAATCCTTGTT *F GGCAGTATGGTGGCATATATAACCTGTAGTTTTCTGATGATCAGTTTGAGAGACACGACCACATTTTCTATCTCAGCAG *F TGATTAGTTTTTTTTCACCACATTTTATCGTGTGCGCGGTTTCTTTTCTGGCTGGAAATGTAATGATTAAGTTACGCAT *F ATATCTGAGTGCACTTAACGAGGTGTTGAAGAACCTAGCCCATCAATGGGACACCCGAAGCCTCAAGGCAGTGAATCAA *F AAACAGCGCTCTCTACAATGTCTCGATTCATTTTCCATGTACACCATTGTAACCAAGGATCCTGCGGAGATTATACAGG *F AGTCCATGGAGATACATCATCTCATTTGCGAGGCAGCTGCCACGGCTAACAAATATTTTACCTACCAATTGCTGACCAT *F TATATCCATAGCATTTCTGATCATCGTTTTCGATGCATACTATGTTCTGGAGACGCTACTGGGAAAATCGAAGCGCGAA *F AGCAAATTCAAAACTGTGGAATTTGTGACATTTTTCTCGTGTCAAATGATTTTGTATCTGATCGCCATAATTTCCATTG *F TCGAGGGAAGTAATCGAGCCATCAAAAAGAGCGAGAAAACTGGAGGCATAGTGCACTCCCTACTCAATAAAACCAAAAG *F TGCTGAGGTCAAGGAGAAACTGCAGCAATTCTCCATGCAGTTGATGCATCTGAAAATTAATTTTACTGCAGCTGGTCTG *F TTCAACATCGACCGCACATTGTATTTCACGATCAGCGGGGCCTTGACCACTTATCTCATCATCTTGCTGCAGTTCACAT *F CCAATTCCCCGAACAATGGTTATGGGAATGGCAGCTCTTGCTGTGAGACCTTCAATAATATGACGAATCATACGCTTTAG *F Protein sequence: *F >Gr28bA *F MIRCGLDIFRGCRGRFRYWLSARDCYDSISLMVAIAFALGITPFLVRRNALGENSLEQSWYGFLNAIFRWLLLAYCYSY *F INLRNESLIGYFMRNHVSQISTRVHDVGGIIAAVFTFILPLLLRKYFLKSVKNMVQVDTQLERLRSPVNFNTVVGQVVL *F VILAVVLLDTVLLTTGLVCLAKMEVYASWQLTFIFVYELLAISITICMFCLMTRTVQRRITCLHKVLKNLAHQWDTRSL *F KAVNQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLG *F KSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINF *F TAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28bB *F MSALRRVRKYFISSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNIFIYICLCGFCYISSLRQGESIV *F GYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFSNIGVRVKYSRIFRYSLLVLIFKLLIL *F GVYFVGVFRLLVSLDVTPSFCVCMTFFLQHSVVSIAICLFCVIAFSFERRLSIINQVLKNLAHQWDTRSLKAVNQKQRS *F LQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFK *F TVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGLFNID *F RTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28bC *F MDIEMAKEPVNPTDTPDIEVTPGLCQPLRRRFRRFVTAKQLYECLRPVFHVTYIHGLTSFYISCDTKTGKKAIKKTIFG *F YINGIMHIAMFVFAYSLTIYNNCESVASYFFRSRITYFGDLMQIVSGFIGVTVIYLTAFVPNHRLERCLQKFHTMDVQL *F QTVGVKIMYSKVLRFSYMVLISMFLVNVLFTGGTFSVLYSSEVAPTMALHFTFLIQHTVIAIAIALFSCFTYLVEMRLV *F MVNKVLKNLAHQWDTRSLKAVNQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISI *F AFLIIVFDAYYVLETLLGKSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEV *F KEKLQQFSMQLMHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28bD *F MSFYFCEIFKPRDAFGAEQTLLLYTYLLGLTPFRLRGQAGERQFHLSKIGYLNAFLQLSFFSYCFLAALIEQQSIVGYF *F FKSEISQMGDSLQKFIGMTGMSILFLCSSIRVRLLIHIWDRISYIDDRFLNLGVCFNYPAIMRLRLLQIFLINGVQLGY *F LISSNWMLLGNDVRPIYTAIVAFYVPQIFLLSIVMLFNATLHRLWQHFTVLNQVLKNLAHQWDTRSLKAVNQKQRSLQC *F LDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVE *F FVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGLFNIDRTL *F YFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F >Gr28bE *F MWLLRRSVGKSGNRPHDVYTCYRLTIFMALCLGIVPYYVSISSEGRGKLTSSYIGYINIIIRMAIYMVNSFYGAVNRDT *F LMSNFFLTDISNVIDALQKINGMLGIFAILLISLLNRKELLKLLATFDRLETEAFPRVGVAMHQVAANKKMNRLVIILV *F GSMVAYITCSFLMISLRDTTTFSISAVISFFSPHFIVCAVSFLAGNVMIKLRIYLSALNEVLKNLAHQWDTRSLKAVNQ *F KQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRE *F SKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLLNKTKSAEVKEKLQQFSMQLMHLKINFTAAGL *F FNIDRTLYFTISGALTTYLIILLQFTSNSPNNGYGNGSSCCETFNNMTNHTL *F Comments: The current Gr28b annotation of 1227aa has unfortunately not been *F revised according to my earlier proposal, so I renew it. This is an *F alternatively spliced splice gene, like Gr23a, except here there are five *F alternative first exons, each presumably with their own promoters and encoding *F quite different TM1-4 domains from each other. They are all proposed to splice *F into a common pair of C-terminal exons that encode the TM5-7 domains of these *F 7TM receptors. I've included the full proposed cDNAs for each of the splice *F forms and proteins (A-E). Comparison with D. pseudoobscura shows exactly the *F same gene structure there. # *U FBrf0161498 *a Robertson *b H. *t 2003.6.23 *T personal communication to FlyBase *u FlyBase error report for Or98P on Mon Jun 23 21:25:29 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 23 Jun 2003 21:25:29 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for Or98P on Mon Jun 23 21:25:29 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: Or98P *F Release: 3 *F Missed gene *F Gene cDNA sequence: *F >Or98P *F ATGTTGTTTAGGTACTTGCGAAAACCTATTGCTCAGGATCTCCTGACTTCTCCGGAGGCATGGCGCTACTTTGAGTATGG *F CATGTTTTGGATGGGATGGATGGCTCCGCCAAAGAACCGGGGCCTAATTCAATATATCGTGGCTAAGCTGGCATTTTCAG *F TAGTTACTTTTGTCAAACTGAACATTTCTGAGCGATTGACAAAAAATTAG *F Protein sequence: *F >Or98P *F MLFRYLRKPIAQDLLTSPEAWRYFEYGMFWMGWMAPPKNRGLIQYIXVAKLAFSVVTFVKLNISERLTKN *F Comments: This is a pseudogene related to Or98a, but which has suffered a *F large internal deletion. What remains is the N-terminus, part of the last *F intron and the last exon. The X in the 'translation' marks the deletion. # *U FBrf0161499 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for CG17868 on Tue Jun 24 18:04:29 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:04:29 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG17868 on Tue Jun 24 18:04:29 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG17868 *F Release: 3 *F Gene annotation error *F Gene CG17868 has incorrect exon/intron structure or translation start site. *F Comments: The correct full-length transcript is PB. I don't understand what *F the evidence for PA is, and since it removes the long second exon it encodes a *F protein without 7TM domains that is unlikely to be a functional receptor. *F Unfortunately the PA form comes up as the default version of the gene, which I *F think is misleading. # *U FBrf0161500 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for CG1978 on Tue Jun 24 18:07:49 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:07:49 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG1978 on Tue Jun 24 18:07:49 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG1978 *F Release: 3 *F Gene annotation error *F Gene CG1978 has incorrect exon/intron structure or translation start site. *F Comments: The PA form of this annotation is correct in my book, but the PB *F form does not have the final exon, which encodes the TM7 domain. This is *F unlikely to be a functional receptor and I'm unsure as to the basis for this *F annotation \- it might be a hangover from the automated annotations, which *F commonly missed the C-terminal final exon of these chemoreceptor genes. # *U FBrf0161501 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for Gr22bP on Tue Jun 24 18:27:17 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:27:17 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for Gr22bP on Tue Jun 24 18:27:17 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: Gr22bP *F Release: 3 *F Missed gene *F Gene cDNA sequence: *F >Gr22bP *F ATGTTTGGTTCAAGTCGCGAAATTCGTCCTTATTTGGCAAGACAGATGCTGAAAACCACACTTTATGGATCCTGGCTACT *F CGGTATATTCCCCTTTACCCTCGACTCCGGAAAAAGAATACGTCAACTGAGACGATCCCGCTGCCTAACTTTGTACGGCC *F TGGTACTTAATTACTTCCTTATATTTACTCTGATTTGATTGGCTTTTGAATACCGGAAACATAAACTAGAAGCCTTTAAG *F CGAAATCCTGTGCTGGAGATGATAAATGTAGTAATTGGAATTATAAACGTTCTTTCAGCCCTGATTGTACACTTTATGAA *F CTTTTGGGGCAGCAGAAAAGTTGGTGAGATATGCAACGAACTTTTAATTCTTGAGTATCAAGACTTTGAAGGCCTTAATG *F GGAGAAATTGCCCAAATTTCAACTGCTTTGTGATTCAAAAGTGTTTGACAATATTGGGCCAGCTGTTATCTTTCTTCACG *F CTCAACTTCGCACTTCCGGGCCTTGAGTTTCATATATGTCTAGTACTTCTGAGCTGCCTGATGGAGTTTTCGCTCAATTT *F GAACATTATGCACTACCATGTCGGTGTCCTGTTAATCTATCGCTATGTATGGCTGATAAATGAACAACTGAAGGACCTAG *F TGAGCCAATTAAAACTGAATCCTGAGACAGACTTCTCCAGAATACATCAGTTTCTATCCTTATACAAACGCCTTCTAGAG *F CTAAATAGAAAATTGGTGATCGCCTACGAATACCAAATGACCCTATTTATAATAGCTCAGCTATCTGGAAACATCGTGGT *F CATTTATTTCCTCATTGTATATGGATTAAGTATGCGTACTTATTCTATCTTCTTGGTGGCATTCCCAAATTCCCTGTTAA *F TAAACATTTGGGATTTCTGGTTGTGCATTGCCGCATGCGATCTTACCGAAAAGGCTGGTGACGAAACCGCGATAATCCTA *F AAAATATTTTCTGACCTCGAGCACAGAGATGATAAGCTAGAAATGAGTGTTAACGAGTTCGCTTGGCTTTGCAGTCACCG *F GAAGTTCCGATTTCAACTGTGTGGACTATTTTCTATGAACTGTAGAATGGGCTTCAAAATGATCATAACCACTTTCCTGT *F ACTTAGTATATTTAGTTCAGTTTGATTATATGAACTTGTAA *F Protein sequence: *F >Gr22bP *F MFGSSREIRPYLARQMLKTTLYGSWLLGIFPFTLDSGKRIRQLRRSRCLTLYGLVLNYFLIFTLIZLAFEYRKHKLEAFK *F RNPVLEMINVVIGIINVLSALIVHFMNFWGSRKVGEICNELLILEYQDFEGLNGRNCPNFNCFVIQKCLTILGQLLSFFT *F LNFALPGLEFHICLVLLSCLMEFSLNLNIMHYHVGVLLIYRYVWLINEQLKDLVSQLKLNPETDFSRIHQFLSLYKRLLE *F LNRKLVIAYEYQMTLFIIAQLSGNIVVIYFLIVYGLSMRTYSIFLVAFPNSLLINIWDFWLCIAACDLTEKAGDETAIIL *F KIFSDLEHRDDKLEMSVNEFAWLCSHRKFRFQLCGLFSMNCRMGFKMIITTFLYLVYLVQFDYMNL *F Comments: This gene was recognized as a young pseudogene by Dunipace et al *F (2001), but Scott et al (2001) avoided the stop codon in the first exon by *F starting their annotation later (which is unsatisfactory because then the *F protein doesn't have a TM1 region comparable to Gr22a, c, e, and f. I think it *F would be good to somehow annotate this in FlyBase as Gr22bP. We have amplified *F and sequenced the first exon from an Oregon R strain and find that the stop *F codon is a CGA encoding arginine there. So like Or85eP this appears to be a *F polymorphic pseudogene in the species. # *U FBrf0161502 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for Gr22dP on Tue Jun 24 18:32:54 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:32:54 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for Gr22dP on Tue Jun 24 18:32:54 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: Gr22dP *F Release: 3 *F Missed gene *F Gene cDNA sequence: *F >Gr22dP *F ATGTTCCGACCTCGATGTGGTCTTCGACAGAAGTTTGTCTACGTTATCCTCAAGTCGATCCTTTATAGCTCCTGGCTGCT *F TGGAATATTTCCATTTAAGTATGAACCCAAAAAGAGGAGATTGCGTCGTTCTATGTGGCTAATCC*TTTTGGAGTCGTCA *F TAAGTTCCAGTCTCCTTATTCTGATGGTAAAACAGAGTGCAGAGGACAGAGAGCATGGGATCATGTTGGACGTCTTTCAA *F AGGAACGCCCTGCTCTATCAAATTAGTTCGCTAATGGGAGTAGTCGGAGTGGTGAGCATTTGTACGGTGCACTTGCGAAC *F CCTTTGGAGGAGCAAACACCTGGAGGAGATCTACAATGGATTAATGCTGCTGGAAGCCAAGTACTTTTGCTCTAATGCAG *F TGGAGTGTCCCGCCTTCGATGGTTATGTAATCCAGAAAGGAGTCGTGATTGTCGTGGGACTATTAGCTCCATGGATGGTG *F CACTTTGGAATGCCAGATTCTAAATTGCCGGTACTGAACGTCTTGGTCGTCTCGATGGTCAAGTTGGGAACCCTTCTCCT *F GGCCCTCCATTATCATCTTGGGGTGGTTATTATTTACCGATTTGTGTGGCTTATAAACAGGGAACTCCTAAGCCTGGTTT *F GTTCCTTGAGGGGAAACCATAAGGGCAGCTCCTCCAGAGTTCGCTTTCTCCTGAAACTCTACAATAAGCTGGTGAATCTA *F TATAGCAAACTGGCCGATTGCTACGACTGCCAGACGGTCCTGATGATGGCTATCTTTCTAGCTGCCAATATCATCGTTTG *F CTTCTATATGATCGTCTACAGGATCAGCCTGAGCAAGATGTCCTTTTTCGTAATGCTAATTATGTTTCCCCTTGCAATAG *F CTAATAATTTCATGGACTTTTGGCTGAGCATGAAGGTTTGCGACTTATTGCAGAAAACTGGAAGACAAACTTCAATGATC *F TTAAAGCTGTTTAATGATATTGAGAATATGGACAAGGATCTTGAGATAAGTATATCAGACTTTGCCTTGTACTGCAGCCA *F TCGTAGATTTAAGTTCCTTCACTGCGGACTATTTCATGTGAATCGAGAAATGGGCTTTAAAATGTTCGTCGCAAGCGTTC *F TCTACTTGCTGTACCTGGTCCAGTTCGACTATATGAACTTGTGA *F Protein sequence: *F >Gr22dP *F MFRPRCGLRQKFVYVILKSILYSSWLLGIFPFKYEPKKRRLRRSMWLIXFGVVISSSLLILMVKQSAEDREHGIMLDVFQ *F RNALLYQISSLMGVVGVVSICTVHLRTLWRSKHLEEIYNGLMLLEAKYFCSNAVECPAFDGYVIQKGVVIVVGLLAPWMV *F HFGMPDSKLPVLNVLVVSMVKLGTLLLALHYHLGVVIIYRFVWLINRELLSLVCSLRGNHKGSSSRVRFLLKLYNKLVNL *F YSKLADCYDCQTVLMMAIFLAANIIVCFYMIVYRISLSKMSFFVMLIMFPLAIANNFMDFWLSMKVCDLLQKTGRQTSMI *F LKLFNDIENMDKDLEISISDFALYCSHRRFKFLHCGLFHVNREMGFKMFVASVLYLLYLVQFDYMNL *F Comments: Like Gr22bP, this appears to be a strain-specific young pseudogene. *F Noted by Dunipace et al. (2001) and again avoided by Scott et al. (2001) by *F starting the translation later, there is a single base deletion in a string of *F four Ts (indicated by the asterisk in the cDNA above), as shown by *F amplification and sequencing of the region from Oregon-R. The deleted base is *F another T, hence the X in the amino acid sequence for the intact allele is *F proline. As for Or85e and Gr22b I think it would be good to somehow include *F this annotation in GadFly/FlyBase. # *U FBrf0161503 *a Muller *b B. *t 2003.7.1 *T personal communication to FlyBase *u FlyBase error report for CG4041 on Tue Jul 1 08:30:20 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 1 Jul 2003 08:30:20 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: B.Mueller@molbio.unizh.ch *F Subject: FlyBase error report for CG4041 on Tue Jul 1 08:30:20 2003 *F Error report from Bruno Müller (B.Mueller@molbio.unizh.ch) *F Gene or accession: CG4041 *F Release: 3 *F Gene annotation error *F Gene CG4041 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F CAACGTGAACAGCTGTTTTTGCATTCCGCTCGCAAAAAAAAAAGAAAAAAAAATATTAAATTGTTGCTGATGCTGGCGT *F GAAGTCCAAGGAAATCGCTGTGATTTGCAAATCGCATCGAGATCATGGGCACGCGGGAGCGCGAAAGGGAGTGCCGCCT *F GTGCGCGGTCACCTTCTTCGCGAAACTGCATCCCGGCGACGTGTGCGGCAGCAACGGCCTGCCCCTAACGCCCAACTCG *F ATTGCGATCCTGGGGCGTGCCCAGAAACTGAAGGAGCTGCAGGACGAGCACCTGTGCCAGTACCTGGATGTGATCCGCG *F GCAAACACGAGCGCACCATTGTGGTTTCGGAGTATCTGGGACTCTCACTGGAGGACTACGCCATGCGTCATCCACCGCT *F GGCCATTGCCCAGATACTGAGAATCTTCTACCAGGTGGCGTGCGGCATCAATGTGCTGAGTCGACACCATCTGGTGGCC *F CACAACGTGGAGCCCAAGCACATTCTACTCTCCAGCGATGGGCAGCGGGTGAAGCTCTTCAACTACGGGCTGCATCACA *F TGACCAAGGGCGGTGCCTATGTGCCCTTTCCCATTGGCAACATTCGCTACATGGCACCCGAACGACTGCTCGGTCTGAA *F TGGCAATGTGAAGAGCGACGTTTGGTCGCTGGCCCTGGTAATGGTGGAGCTAATATTGCAGATCGAGCTGTGGCCGAAG *F CTCAAGCTCTCGAATGTGGTTAGAAAGATCCTGGCCTTTGGCAAGAGCAACGGGGCGTTGGAGAAGATTGCGCGAGAGC *F ACCAGTGTCACGAGCGCTATGTGCAGATGGATCAGCGCCTGCGACAGCTGCTGGAGAGCTGTTTAAGTGTACTGCCCAA *F GAGGAGACCACTGCCAGGTGAGCTCTTGGAACATCCCATTTTCGAGGAAGTCCTATTGGATTTGAAGAAACAGAAGATG *F CAGCCACTGAGCCCAGAAACCGAACATTTACCGCTGCTGCTGCGATGTCCCTTGTCGC *F AGATCTACCACCTGTGGCAACTGGCCGGCGGAGATGTGCAGGCGGAGCTGAAAAAGGAGGGCCTCATCCGGAGTGAGGC *F ACCCATTTTGGGTCTGCCACAAATCGTTCGCCTGAGCGGCGCCAGCGTTTGTCCCGGCCGAAGCCAGGCGCAGCTGATG *F GACGATCGCGTGGTGCCGCTGCGCCTGAAGGCGCTGCTCCAGCGACTGAGTGGCCTGCCGGCCGCAGTCTACTTTCCCC *F TGCTCCACTCGCCGCGTTTTCCCGCCCACTTTGCCCGCGAATTGCAGGAACTGCCGCTGGTGATCCGCGAAAAGGACAT *F CGAGTATCAGTTTCAGCGCGTGCGACTTTTTGCGCGCCTGCTCCAGGGCTATCCGCATACGGCGGAGCAGCTGCAGCGC *F GAGGCAGCCGTGGATGTGCCGCCGCTACTGAGGGGTCCCATTTGGGCCGCCCTGCTGGAGGTTGTGCCCAATGGCAGTT *F ACGCGAAGATCGACAAGTTTACGTCTACGAGCACGGATCGGCAGATCGAGGTGGATATACCGCGCTGCCATCAGTACGA *F TGAGCTGCTCTCCTCGCCGGATGGCCACCGCAAGCTGAGGCGGCTGCTCAAGGCCTGGGTCACCGCCCATCCGCAGTAC *F GTCTACTGGCAGGGACTGGACTCACTGACGGCACCGTTTCTCTATCTCAACTTTAACAATGAAGAGTTGGCCTTCCTCA *F GCTTGTTCAAGTTCATTCCAAAGTATCTGCAATGGTTCTTTCTCAAGGATAATTCGGCGGTGATCAAGGAGTACCTGAG *F CAAGTTCTCCCAGCTGACTGCCTTCCATGAGCCGCTTCTCGCTCAGCATTTGGCCAGCATTAGCTTTATACCCGAGTTG *F TTTGCCATCCCGTGGTTCCTAACCATGTTCTCGCATGTATTCCCGCTGCACAAGATCCTGCATTTGTGGGATAAACTCA *F TGCTGGGCGATAGTTCGTATCCG *F CTGTTCATTGGCATCGCCATATTGCGCCAGCTGAGAAGCACGCTGCTCACCTCCGGTTTCAACGAGTGCA *F TCCTGCTCTTCTCCGACCTGCCCGATATCGTGATGGATGGCTGTGTGCTGGAGTCACAGAAGATGTACGAAGCCACACC *F GAAGAGTATTACTCATCGCCAGCACGCGTTGCGCCTCCAGCCGCCACAGGCGTTGGACATTGGCGTGGCGGATGTCGAG *F CTGAAGCACCTGCAACAGGAGCAGTGCCCCCGGATCAGCGCCAAGGATGTGCAATTCCTGTTGGACAATTCGCCAGCGG *F AGCTGGCCCTCATCGATTTGCGCAGTGTAGTCGAGTTCGGACGCGTCCATGTGCCGCATAGCATCAACATACCGTTCGC *F CACCGTCCAGTTGGGTGAACAGCGATTGGAGGCCCTGCAGGTGCCCCAATTGGAGGCGCAGCTCCGCGGCAAGATCGTC *F GTCTGCGTGAGCAATATCCATCAGCACTCCGTGGAGTTCTCGCACTTTCTGGTGGCCTGCGGTGTCCAGCGCACCTGTA *F TCCTTCACAAAGGATTCAACGTCCTGCACTCCATCGAGCCAAATATACTCATCTCGAATTGA *F Protein sequence: *F MGTRERERECRLCAVTFFAKLHPGDVCGSNGLPLTPNSIAILGRAQKLKELQDEHLCQYLDVIRGKHERTIVVSEYLGL *F SLEDYAMRHPPLAIAQILRIFYQVACGINVLSRHHLVAHNVEPKHILLSSDGQRVKLFNYGLHHMTKGGAYVPFPIGNI *F RYMAPERLLGLNGNVKSDVWSLALVMVELILQIELWPKLKLSNVVRKILAFGKSNGALEKIAREHQCHERYVQMDQRLR *F QLLESCLSVLPKRRPLPGELLEHPIFEEVLLDLKKQKMQPLSPETEHLPLLLRCPLSQIYHLWQLAGGDVQAELKKEGL *F IRSEAPILGLPQIVRLSGASVCPGRSQAQLMDDRVVPLRLKALLQRLSGLPAAVYFPLLHSPRFPAHFARELQELPLVI *F REKDIEYQFQRVRLFARLLQGYPHTAEQLQREAAVDVPPLLRGPIWAALLEVVPNGSYAKIDKFTSTSTDRQIEVDIPR *F CHQYDELLSSPDGHRKLRRLLKAWVTAHPQYVYWQGLDSLTAPFLYLNFNNEELAFLSLFKFIPKYLQWFFLKDNSAVI *F KEYLSKFSQLTAFHEPLLAQHLASISFIPELFAIPWFLTMFSHVFPLHKILHLWDKLMLGDSSYPLFIGIAILRQLRST *F LLTSGFNECILLFSDLPDIVMDGCVLESQKMYEATPKSITHRQHALRLQPPQALDIGVADVELKHLQQEQCPRISAKDV *F QFLLDNSPAELALIDLRSVVEFGRVHVPHSINIPFATVQLGEQRLEALQVPQLEAQLRGKIVVCVSNIHQHSVEVGHPL *F AQLKLLRTCILHKGFNVLHSIEPNILISN *F Comments: according to sequenced EST clone LD35883, and alignment information *F obtained when compared to Anopheles, human and murine homolgs, a stretch of *F the following sequence is not translated and hence intronic: *F GGTGGGGCATCCACTGGCGCAATTGAAGCTTCTTTGCAAACTAAAGTCATTCCCTATTTCGCA # *U FBrf0161504 *a DiMario *b P.J. *t 2003.7.23 *T personal communication to FlyBase *u FlyBase error report for CG7421 on Wed Jul 23 06:44:22 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 23 Jul 2003 06:44:22 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: pdimari@lsu.edu *F Subject: FlyBase error report for CG7421 on Wed Jul 23 06:44:22 2003 *F Error report from Patrick J. DiMario (pdimari@lsu.edu) *F Gene or accession: CG7421 *F Release: 3 *F Gene annotation error *F Gene CG7421 has incorrect exon/intron structure or translation start site. *F Comments: The predicted transcript, CG7421-RC, shows a small intron of 96 bp *F beginning with GCAG... and ending with ...AAG. This 96 bp sequence is *F correct, but it should not be shown as an intron. It should be a part of the *F larger exon. The error is ours. When we posted our original cDNA sequence we *F inadvertently left this 96 bp sequence block out due to its highly repetitive *F nature within the overall cDNA sequence. Our accession number is AF162774; it *F has been corrected to show the proper coding sequence. *F Also, the existence of transcript CG7421-RA would be very surprising. *F CG7421-RB encodes a close homologue to the mammalian Nopp140, and this protein *F is expected to exist in Drosophila. CG7421-RC encodes a Nopp140 isomer with a *F distinctive glycine and arginine carboxy rich tail that has been found in *F other nucleolar proteins, so we also expect this isomer to exist. But the *F predicted protein product from transcript CG7421-RA would be totally unexpected. # *U FBrf0161505 *a Robertson *b H. *t 2003.6.25 *T personal communication to FlyBase *u FlyBase error report for CG17849 on Tue Jun 24 18:14:43 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:14:43 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG17849 on Tue Jun 24 18:14:43 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG17849 *F Release: 3 *F Gene annotation error *F Gene CG17849 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Or46aA *F ATGAGCAAAGGAGTAGAAATCTTTTACAAGGGCCAGAAGGCATTCTTGAACATCCTCTCGTTGTGGCCTCAGATAGAAC *F GCCGGTGGAGAATCATCCACCAGGTGAACTATGTCCACGTAATTGTGTTTTGGGTGCTGCTCTTTGATCTCCTCTTGGT *F GCTCCATGTGATGGCTAATTTGAGCTACATGTCCGAGGTTGTGAAAGCCATCTTTATCCTGGCCACCAGTGCAGGGCAC *F ACCACCAAGCTGCTGTCCATAAAGGCGAACAATGTGCAGATGGAGGAGCTCTTTAGGAGATTGGATAACGAAGAGTTCC *F GTCCTAGAGGCGCCAACGAAGAGTTGATCTTTGCAGCAGCCTGTGAAAGAAGTAGGAAGCTTCGGGACTTCTATGGAGC *F GCTTTCGTTTGCCGCCTTGAGCATGATTCTCATACCCCAGTTCGCCTTGGACTGGTCCCACCTTCCGCTCAAAACATAC *F AATCCGCTTGGCGAGAATACCGGCTCACCTGCTTATTGGCTCCTCTACTGCTATCAGTGTCTGGCCTTGTCCGTATCCT *F GCATCACCAACATAGGATTCGACTCACTCTGCTCCTCACTGTTCATCTTCCTCAAGTGCCAGCTGGACATTCTGGCCGT *F GCGACTGGACAAGATCGGTCGGTTAATCACTACTTCTGGTGGCACTGTGGAACAGCAACTTAAGGAAAATATCCGCTAT *F CACATGACCATCGTTGAACTGTCGAAAACCGTGGAGCGTCTACTTTGCAAGCCGATTTCGGTGCAGATCTTCTGCTCGG *F TTTTGGTGCTGACTGCCAATTTCTATGCCATTGCTGTGTTATCTGACGAGAGGCTGGAGCTCTTTAAGTATGTGACCTA *F TCAGGCGTGCATGTTGATTCAGATTTTTATATTGTGCTACTATGCCGGTGAGGTAACCCAGCGCAGCCTGGACCTTCCG *F CACGAGCTGTACAAGACCTCCTGGGTGGACTGGGACTACAGGAGCCGAAGGATTGCGCTCCTCTTTATGCAACGCCTTC *F ACTCGACCTTGAGGATTAGGACACTTAATCCAAGTCTTGGTTTTGACTTAATGCTCTTCAGCTCGATCGTCAACTGCTC *F CTACAGCTACTTCGCACTGCTGAAGCGCGTCAACAGTTAA *F >Or46aB *F ATGGTTACGGAGGACTTTTATAAGTACCAGGTGTGGTACTTCCAAATCCTTGGTGTTTGGCAGCTCCCCACTTGGGCCG *F CAGACCACCAGCGTCGTTTTCAGTCCATGAGGTTTGGCTTCATCCTGGTCATCCTGTTCATCATGCTGCTGCTTTTCTC *F CTTCGAAATGTTGAACAACATTTCCCAAGTTAGGGAGATCCTAAAGGTATTCTTCATGTTCGCCACGGAAATATCCTGC *F ATGGCCAAATTATTGCATTTGAAGTTGAAGAGCCGCAAACTCGCTGGCTTGGTTGATGCGATGTTGTCCCCAGAGTTCG *F GCGTTAAAAGTGAACAGGAAATGCAGATGCTGGAATTGGATAGAGTGGCGGTTGTCCGCATGAGGAACTCCTACGGCAT *F CATGTCCCTGGGCGCGGCTTCCCTGATCCTTATAGTTCCCTGTTTCGACAACTTTGGCGAGCTACCACTGGCCATGTTG *F GAGGTATGCAGCATCGAGGGATGGATCTGCTATTGGTCGCAGTACCTTTTCCACTCGATTTGCCTGCTGCCCACTTGTG *F TGCTGAATATAACCTACGACTCGGTGGCCTACTCGTTGCTCTGTTTCTTGAAGGTTCAGCTACAAATGCTGGTCCTGCG *F ATTAGAAAAGTTGGGTCCTGTGATCGAACCCCAGGATAATGAGAAAATCGCAATGGAACTGCGTGAGTGTGCCGCCTAC *F TACAACAGGATTGTTCGTTTCAAGGACCTGGTGGAGCTGTTCATAAAGGGGCCAGGATCTGTGCAGCTCATGTGTTCTG *F TTCTGGTGCTGGTGTCCAACCTGTACGACATGTCCACCATGTCCATTGCAAACGGCGATGCCATCTTTATGCTCAAGAC *F CTGTATCTATCAGCTGGTGATGCTCTGGCAGATCTTCATCATTTGCTACGCCTCCAACGAGGTAACTGTCCAGAGCTCT *F AGGTTGTGTCACAGCATCTACAGCTCCCAATGGACGGGATGGAACAGGGCAAACCGCCGGATTGTCCTTCTCATGATGC *F AGCGCTTTAATTCCCCGATGCTCCTGAGCACCTTTAACCCCACCTTTGCTTTCAGCTTGGAGGCCTTTGGTTCTATCGT *F CAACTGCTCCTACAGCTACTTCGCACTGCTGAAGCGCGTCAACAGTTAA *F Protein sequence: *F >Or46aA *F MSKGVEIFYKGQKAFLNILSLWPQIERRWRIIHQVNYVHVIVFWVLLFDLLLVLHVMANLSYMSEVVKAIFILATSAGHT *F TKLLSIKANNVQMEELFRRLDNEEFRPRGANEELIFAAACERSRKLRDFYGALSFAALSMILIPQFALDWSHLPLKTYNP *F LGENTGSPAYWLLYCYQCLALSVSCITNIGFDSLCSSLFIFLKCQLDILAVRLDKIGRLITTSGGTVEQQLKENIRYHMT *F IVELSKTVERLLCKPISVQIFCSVLVLTANFYAIAVLSDERLELFKYVTYQACMLIQIFILCYYAGEVTQRSLDLPHELY *F KTSWVDWDYRSRRIALLFMQRLHSTLRIRTLNPSLGFDLMLFSSIVNCSYSYFALLKRVNS *F >Or46aB *F MVTEDFYKYQVWYFQILGVWQLPTWAADHQRRFQSMRFGFILVILFIMLLLFSFEMLNNISQVREILKVFFMFATEISCM *F AKLLHLKLKSRKLAGLVDAMLSPEFGVKSEQEMQMLELDRVAVVRMRNSYGIMSLGAASLILIVPCFDNFGELPLAMLEV *F CSIEGWICYWSQYLFHSICLLPTCVLNITYDSVAYSLLCFLKVQLQMLVLRLEKLGPVIEPQDNEKIAMELRECAAYYNR *F IVRFKDLVELFIKGPGSVQLMCSVLVLVSNLYDMSTMSIANGDAIFMLKTCIYQLVMLWQIFIICYASNEVTVQSSRLCH *F SIYSSQWTGWNRANRRIVLLMMQRFNSPMLLSTFNPTFAFSLEAFGSIVNCSYSYFALLKRVNS *F Comments: There's a complicated problem with the current Or46a/b gene(s). Like *F several of the Grs, we now recognize that they are alternatively spliced, *F starting with different promoters and first exons and sharing only the final *F short exon. So there is one gene, Or46a, with two alternatively transcribed *F transcripts, PA, and PB. Thus the current annotation CG17849-PA should have *F the final exon added, and a PB transcript added for the current 46b. CG17848 *F should be dropped. My cDNAs and proteins are above \- they are confirmed by *F the D. pseudoobscura comparison and by RT/PCR from antennae by Coral Warr *F (ex-postdoc in John Carlson’s lab now in Australia and co-author on our *F manuscript). We call these proteins Or46aA/B. # *U FBrf0161506 *a Robertson *b H. *t 2003.6.24 *T personal communication to FlyBase *u FlyBase error report for CG17902 on Tue Jun 24 18:38:12 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 24 Jun 2003 18:38:12 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG17902 on Tue Jun 24 18:38:12 2003 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG17902 *F Release: 3 *F Gene annotation error *F Gene CG17902 has incorrect exon/intron structure or translation start site. *F Gene cDNA sequence: *F >Or69aA *F ATGCAGTTGCACGACCATATGAAGTACATAGACTTGGGTTGCAAGATGGCATGCATACCAAGATATCAATGGAAAGGACG *F CCCTACTGAAAGACAGTTCTACGCTTCGGAGCAAAGGATAGTGTTCCTTCTTGGAACCATTTGCCAGATATTCCAGATTA *F CTGGAGTGCTTATCTATTGGTATTGCAATGGCCGTCTTGCCACGGAAACGGGCACCTTTGTGGCACAATTATCTGAAATG *F TGCAGTTCTTTTTGTCTAACATTTGTGGGATTCTGTAACGTTTATGCGATCTCTACAAACCGCAATCAAATTGAAACATT *F ACTCGAGGAGCTTCATCAGATATATCCGAGATACAGGAAAAATCACTATCGCTGCCAGCATTATTTTGACATGGCCATGA *F CAATAATGAGAATTGAGTTTCTTTTCTATATGATCTTGTACGTGTACTACAATAGTGCACCATTATGGGTGCTTCTTTGG *F GAACACTTGCACGAGGAATATGATCTTAGCTTCAAGACGCAGACCAACACTTGGTTTCCATGGAAAGTCCATGGGTCGGC *F ACTTGGATTTGGTATGGCTGTACTAAGCATAACCGTGGGATCCTTTGTGGGCGTAGGTTTCAGTATTGTCACCCAGAATC *F TTATCTGTTTGTTAACCTTCCAACTAAAGTTGCACTACGATGGAATATCCAGTCAGTTAGTATCTCTCGATTGCCGTCGT *F CCTGGAGCTCATAAGGAGTTGAGCATCCTCATCGCCCACCACAGCCGAATCCTTCAGCTGGGCGACCAAGTCAATGACAT *F AATGAACTTTGTATTCGGCTCTAGCCTAGTAGGTGCCACTATTGCCATTTGTATGTCAAGTGTTTCTATAATGCTACTGG *F ACTTAGCATCTGCCTTCAAATATGCCAGTGGTCTAGTGGCATTCGTCCTCTACAACTTTGTCATCTGCTACATGGGAACC *F GAGGTCACTTTAGCTAGTGGCAAAGTATTGCCAGCGGCCTTTTATAACAATTGGTATGAAGGCGATCTTGTTTATCGAAG *F GATGCTCCTCATCCTGATGATGCGTGCTACGAAACCTTATATGTGGAAAACCTACAAGCTGGCACCTGTATCCATAACTA *F CATATATGGCAACATTGAAGTTTTCATATCAAATGTTTACCTGTGTGCGGTCCCTTAAATAA *F >Or69aB *F ATGCAGTTGGAGGACTTTATGCGGTACCCGGACCTCGTGTGTCAAGCGGCCCAACTTCCCAGATACACGTGGAATGGCAG *F ACGATCCTTGGAAGTTAAACGCAACTTGGCAAAACGCATTATCTTCTGGCTTGGAGCAGTAAATTTGGTTTATCACAATA *F TTGGCTGCGTCATGTATGGCTATTTCGGTGATGGAAGAACAAAGGATCCAATTGCGTATTTAGCTGAATTGGCATCTGTG *F GCCAGCATGCTTGGTTTCACCATTGTGGGCACCCTCAACTTGTGGAAGATGCTGAGCCTTAAGACCCATTTTGAGAACCT *F ACTAAATGAATTCGAGGAATTATTTCAACTAATCAAGCACAGGGCGTATCGCATACACCACTATCAAGAAAAGTATACGC *F GTCATATACGAAATACATTTATTTTCCATACCTCTGCCGTTGTCTACTACAACTCACTACCAATTCTTCTAATGATTCGG *F GAACATTTCTCGAACTCACAGCAGTTGGGCTATAGAATTCAGAGTAATACCTGGTATCCCTGGCAGGTTCAGGGATCAAT *F TCCTGGATTTTTTGCTGCAGTCGCCTGTCAAATCTTTTCGTGCCAAACCAATATGTGCGTCAATATGTTTATCCAGTTTC *F TGATCAACTTTTTTGGTATCCAGCTAGAAATACACTTCGATGGTTTGGCCAGGCAGCTGGAGACCATCGATGCCCGCAAT *F CCCCATGCCAAGGATCAATTGAAGTATCTGATTGTATATCACACAAAATTGCTTAATCTAGCCGACAGAGTTAATCGATC *F GTTTAACTTTACGTTTCTCATAAGTCTGTCGGTATCCATGATATCCAACTGTTTTCTGGCATTTTCCATGACCATGTTCG *F ACTTTGGCACCTCTCTAAAACATTTACTCGGACTTTTGCTATTCATCACATATAATTTTTCAATGTGCCGCAGTGGTACG *F CACTTGATTTTAACGAGTGGCAAAGTATTGCCAGCGGCCTTTTATAACAATTGGTATGAAGGCGATCTTGTTTATCGAAG *F GATGCTCCTCATCCTGATGATGCGTGCTACGAAACCTTATATGTGGAAAACCTACAAGCTGGCACCTGTATCCATAACTA *F CATATATGGCAACATTGAAGTTTTCATATCAAATGTTTACCTGTGTGCGGTCCCTTAAATAA *F Protein sequence: *F >Or69aA *F MQLHDHMKYIDLGCKMACIPRYQWKGRPTERQFYASEQRIVFLLGTICQIFQITGVLIYWYCNGRLATETGTFVAQLSEM *F CSSFCLTFVGFCNVYAISTNRNQIETLLEELHQIYPRYRKNHYRCQHYFDMAMTIMRIEFLFYMILYVYYNSAPLWVLLW *F EHLHEEYDLSFKTQTNTWFPWKVHGSALGFGMAVLSITVGSFVGVGFSIVTQNLICLLTFQLKLHYDGISSQLVSLDCRR *F PGAHKELSILIAHHSRILQLGDQVNDIMNFVFGSSLVGATIAICMSSVSIMLLDLASAFKYASGLVAFVLYNFVICYMGT *F EVTLASGKVLPAAFYNNWYEGDLVYRRMLLILMMRATKPYMWKTYKLAPVSITTYMATLKFSYQMFTCVRSLK *F >Or69aB *F MQLEDFMRYPDLVCQAAQLPRYTWNGRRSLEVKRNLAKRIIFWLGAVNLVYHNIGCVMYGYFGDGRTKDPIAYLAELASV *F ASMLGFTIVGTLNLWKMLSLKTHFENLLNEFEELFQLIKHRAYRIHHYQEKYTRHIRNTFIFHTSAVVYYNSLPILLMIR *F EHFSNSQQLGYRIQSNTWYPWQVQGSIPGFFAAVACQIFSCQTNMCVNMFIQFLINFFGIQLEIHFDGLARQLETIDARN *F PHAKDQLKYLIVYHTKLLNLADRVNRSFNFTFLISLSVSMISNCFLAFSMTMFDFGTSLKHLLGLLLFITYNFSMCRSGT *F HLILTSGKVLPAAFYNNWYEGDLVYRRMLLILMMRATKPYMWKTYKLAPVSITTYMATLKFSYQMFTCVRSLK *F Comments: Like some of the Gr genes and Or46aA/B, we now believe that this and *F it's unannotated neighbor are a single alternatively spliced gene. They have *F different N-terminal exons and presumably independent promoters, and then *F splice into the same two C-terminal exons. This structure is completely *F conserved in Dp, and the splicing of this current annotated exon into the *F final two exons is confirmed by RT/PCR by Coral Warr. We would very much like *F to have the names modified a bit so they make sense, that is, as indicated in *F the cDNAs and proteins above. # *U FBrf0161507 *a Bossing *b T. *t 2003.8.19 *T personal communication to FlyBase *u *F Date: Tue, 19 Aug 2003 09:43:31 \-0500 *F To: gm119@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{GawB}227 *F The following information was provided to the Bloomington Stock Center by *F Torsten Bossing, Wellcome/Cancer Research UK Institute (8/03). *F P{GAL4}227, described in Bossing et al., 2002 (genesis 34: 123--126; *F FBrf0152361), is a homozygous viable and fertile, third chromosome *F insertion of P{GawB}, which originated in the lab of Cahir O'Kane. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161536 *a Serfas *b M. *t 2003.8.25 *T personal communication to FlyBase *u *F Date: Tue, 26 Aug 2003 10:02:39 \-0400 (EDT) *F From: Andy Schroeder *F Subject: Re: Extra protein in 'chromosome bows' mRNA *F To: flybase-help@morgan.harvard.edu, msserfas@wisc.edu *F Dear Mike, *F Thank you for bringing this to our attention. After examining the evidence that *F was used to produce this annotation, it is clear that you are absolutely correct *F and we missed the Drosophila gene encoding EC 6.3.5.4 and incorporated it as a *F 5'UTR of chb (bow). *F The evidence suggests that this is not a case of a dicistronic transcript but *F rather that EC 6.3.5.4 is nested within an intron of one of the alternative *F transcripts of chb (bows). The alternative transcript of chb that I am *F referring to is not currently present in the FlyBase annotation report but is *F supported by the LD20859 cDNA. This transcript has a first exon consisting of *F 5'UTR that is 5' to the intronless EC 6.3.5.4 gene. *F Based on current evidence, the annotation for EC 6.3.5.4 will essentially *F consist of the first exon of the current CG32435-RB transcript as supported by *F your tblastn result. *F Currently, because we are working hard on integrating the FlyBase data *F structures, we are in an annotation hiatus. However, we expect to be making a *F new release of annotations before years end and if all goes well this correction *F should appear as a product of this new release. *F I hope this is clear and please let me know if we can provide any additional *F information. *F cheers, *F Andy Schroeder *F for FlyBase *F >Date: Mon, 25 Aug 2003 18:56:21 \-0500 *F >From: Mike Serfas *F >Subject: Extra protein in 'chromosome bows' mRNA *F >To: flybase-help@morgan.harvard.edu *F > *F >To whom it may concern: *F > *F > I was trying to look up EC 6.3.5.4 (which interconverts aspartate *F >and asparagine) in KEGG, which yielded an entry for Anopheles but not *F >Drosophila. *F >http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids *F =21293942&dopt=GenPept *F > *F >Curious, I tried a B-link from this in NCBI, finding no Drosophila *F >homologue. So I did a tblastN restricted to Drosophila and found the *F >following alignment: *F > *F >>gi|24667859|ref|NM_168882.1| Drosophila melanogaster chromosome *F >>bows CG32435-PB (chb) mRNA, *F > complete cds *F > Length = 6844 *F > *F > Score = 614 bits (1583), Expect = e-175 *F > Identities = 317/535 (59%), Positives = 387/535 (72%), Gaps = 3/535 (0%) *F > Frame = \+1 *F > *F >Query: 2 SLREMAFRQSSKQRHRGPDQTGLCVNDEYGFVLVQERLSIVGVKTGRQPLTSTNGMTHLV 61 *F > SLRE+A+RQS K RHRGPD TG+ VN G \++ ERL I+GV+ G QP S \+G LV *F >Sbjct: 76 SLRELAYRQSGKHRHRGPDSTGVYVNSLEGVAMIHERLRIIGVEMGDQPFVSEDGNLILV 255 *F > *F >Query: 62 ANGEIYNFLHMASLINDALKFEYYTPRSDCDVLVAFYELFGADRLLQIVRGMFAFVLYDC 121 *F > ANGEIYN+L \+++ I A K Y P SDC V++ Y+ \+G D LLQ \+ GMFAF LYD *F >Sbjct: 256 ANGEIYNYLELSAEI--AKKRGSYNPMSDCHVILELYQDYGKD-LLQYITGMFAFALYDR 426 *F > *F >Query: 122 STHRLLVARDPVGIVPLYYGWDDAGSLWFASELKCLVGCCPEVNVFPPGHSYYGLRESFV 181 *F > T \+L+ARDP GI+P+Y G D \+G+LW ASE+KCLV C \+V F PG \+ \+G F *F >Sbjct: 427 KTKEVLLARDPFGIIPMYVGEDASGNLWVASEMKCLVDTCSKVETFTPGEARFGKVGDFK 606 *F > *F >Query: 182 PKPYFQALWMTEISTARVDLELLKHHLESAVESHLQSEVPFGALLSGGLDSSLIASIATR 241 *F > FQ W+ E+ T \+L LL+ \+LE AV SHLQ \+V GALLSGG+DSSLIASIAT+ *F >Sbjct: 607 TCWQFQQSWIKEVPTQTCELSLLRANLEFAVRSHLQCDVQMGALLSGGVDSSLIASIATK 786 *F > *F >Query: 242 ILRARTDNSTFRLKTYSIGLEGESPDLTYARMVADYIGSDHTEVRFTIPEGIDLVRDAVY 301 *F > I+R R N FRLKT+S+GL \++PD AR VA YI SDH E+ F I E \+D \+RD \+Y *F >Sbjct: 787 IMRERDPN--FRLKTFSVGLR-DAPDFQAARSVAKYIDSDHKEIIFEIDEALDGIRDIIY 957 *F > *F >Query: 302 YAETYDVTTIRCIVAVMLLARAIRSEGIKVVLSGEGADELFGGYLYFHRAPSAAEFHRET 361 *F > \+ ETYDVTT+RC \+ \++LLAR I+S GIK++LSGEGADE+FGGYLYFH+APS \+FH E *F >Sbjct: 958 HLETYDVTTVRCSLPMLLLARYIKSTGIKMILSGEGADEIFGGYLYFHKAPSYNDFHEEL 1137 *F > *F >Query: 362 VRRVRGLHLSDCLRANKGCAAWGLELRVPFLDTDFLQHVMSIRPEDRAPGIPS---HDDH 418 *F > V+RVR LHLSDCLRANK A G+ELRVPFLDT F+ HVM IRPED+ PG \+ *F >Sbjct: 1138VKRVRQLHLSDCLRANKVAMAKGVELRVPFLDTGFVNHVMQIRPEDKIPGPLNKFGEVQQ 1317 *F > *F >Query: 419 SALEKRILRQAFADGNYLPSEVLWRQKEQFSDGVGYAWIDAIAQWASKRVTQTEYAAAAD 478 *F > LEK \+LR AFAD NYLP EVLWRQKEQFSDGVGY WID+I \+ A+ V+ E++AAA *F >Sbjct: 1318KRLEKYVLRAAFAD-NYLPDEVLWRQKEQFSDGVGYDWIDSIRRVATSHVSDQEFSAAAL 1494 *F > *F >Query: 479 RFPFNTPTTKEAFYYRKLFEQMFPGESFARTVQRWIPRTDWGCSEDPSGRKQDVH 533 *F > RFPFNTPTTKEAFYYR \+F \+ FPGES ARTV RW+PR DWGC EDPSGR Q VH *F >Sbjct: 1495RFPFNTPTTKEAFYYRCIFAEQFPGESAARTVVRWVPRLDWGCPEDPSGRAQAVH 1659 *F > *F >The annotation for this mRNA suggests that a different protein begins *F >far into the sequence: *F > *F > CDS 1699..6174 *F > /gene='chb' *F > /locus_tag='CG32435' *F > /note='chb gene product from transcript CG32435-RB' *F > /codon_start=1 *F > /product='chromosome bows CG32435-PB' *F > /protein_id='NP_730596.1' *F > /db_xref='GI:24667860' *F > /db_xref='FLYBASE:FBgn0021760' *F > /db_xref='LocusID:43901' *F > /translation='MAYRKPSDLDGFIQQMPKA *F > *F >Now, I haven't tried to figure out if the apparent dicistronic *F >transcript is genuine or some type of artifact, but it seems that *F >there's a fly 6.3.5.4 enzyme not currently accessible as a protein *F >sequence. Is there a way for you to fix this? *F > *F >Thanks, *F > *F >Mike *F > *F >msserfas@facstaff.wisc.edu or michael@serfas.net *F >(608)262-7898 (voice) (608)262-9343 (FAX) *F >Michael Serfas, Ph.D., *F >Carroll Lab, Rm 205 Bock *F >University of Wisconsin-Madison, *F >Laboratory of Molecular Biology *F >1525 Linden Drive, Madison WI 53706 *F > *F >-- # *U FBrf0161537 *a Hoppe *b P. *t 2003.8.26 *T personal communication to FlyBase *u FlyBase error report for CG6114 on Mon Aug 25 14:03:29 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Mon, 25 Aug 2003 14:03:29 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: phoppe@genetics.wustl.edu *F Subject: FlyBase error report for CG6114 on Mon Aug 25 14:03:29 2003 *F Error report from Pamela Hoppe (phoppe@genetics.wustl.edu) *F Gene or accession: CG6114 *F Release: 3 *F Gene annotation error *F Gene CG6114 has incorrect exon/intron structure or translation start site. *F Comments: The CG6114 protein sequence begins in the middle of the serine *F kinase domain. In a paper reporting the worm homolog, SAD-1 (Crump, Zhen, Jin, *F and Bargmann, 2001 Neuron 29: 115-129.), the full-length sequence for fly is *F given and called CG6114, although additional N-terminal residues are shown in *F their alignment figure. I have typed a file from their alignment that gives *F the N-terminal residues, but I can't manage to get flybase to accept my file *F when I paste it into the box for corrected protein sequence. *F Date: Tue, 26 Aug 2003 09:39:56 \-0500 *F To: Gillian Millburn (Genetics) *F From: Pam Hoppe *F Subject: Re: FlyBase error report for CG6114 on Mon Aug 25 14:03:29 2003 *F Dear Gillian, *F Here is the sequence file. Given that I typed it in from their figure, I can't *F swear I got it correct. *F Pam *F >DmCG6114 N-terminal residues from SAD-1 paper to end of kinase domain *F MQKENNVTAENCQFVGPYRLEKTLGKGQTGLVKLGVHCVIGKKVAIKIINREKLSESVLMKVEREIAIMKL *F IDHPHVLGLSDVYENKKYLYLILEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLK *F PENLLLDEKNNIKIADFGMASLQPAGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGAL *F PFDDDNLRQLLEKVKRGVFHIPHFVPPDCQSLLRGMIEVNPDRRLTLAEINRHPWV *F At 09:48 AM 8/26/2003 \+0100, you wrote: *F >Dear Pamela, *F > *F >thanks for the update about CG6114. *F > *F >Do you still have the file of sequence you typed in ? If so, can you *F >send it to me and I will make your e-mail below and the sequence a *F >personal communication to FlyBase.=A0 The molecular curators will then *F >look at the new information and can use it to adjust the gene model for *F >CG6114 if they see fit, *F > *F >thanks, *F > *F >Gillian *F > *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- # *U FBrf0161538 *a Hoppe *b P. *t 2003.8.26 *T personal communication to FlyBase *u FlyBase error report for CG11870 on Tue Aug 26 08:55:35 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 26 Aug 2003 08:55:35 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: phoppe@genetics.wustl.edu *F Subject: FlyBase error report for CG11870 on Tue Aug 26 08:55:35 2003 *F Error report from Pamela Hoppe (phoppe@genetics.wustl.edu) *F Gene or accession: CG11870 *F Release: 3 *F Gene annotation error *F Gene CG11870 has incorrect exon/intron structure or translation start site. *F Comments: There are 2 alternative first exons. The last 4 bases of the called *F second exon are part of the intron. The end sequence of exon 2 should be *F TGAACAATCACCGCAAGAAGTTGCGACAAAG. Thus the ORF begins in exon 2 at this *F sequence: ATGGTGATAAGCAAA. The splice of exon 1a (2001-2573) to the corrected *F second exon is supported by EST LP05937.5prime. Exon 1b (28331-28415) to *F corrected second exon is in AT01228.5prime. I have a color-coded word file as *F well as corrected cDNA and protein files, but can't get the boxes here to take *F the fasta files (which work on our Unix machines and on web workbench sites). # *U FBrf0161539 *a Peifer *b M. *t 2003.9.5 *T personal communication to FlyBase *u FlyBase error report for CG17478 on Fri Sep 5 07:22:58 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 5 Sep 2003 07:22:59 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG17478 on Fri Sep 5 07:22:58 2003 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG17478 *F Release: 3 *F Missed gene *F Comments: CG40280 = CG17478 Possible retrotransposon. *F 1) Blast match of predicted protein to BAA95569 RNase H and integrase-like *F protein Bombyx mori. 53% identical over first 30 of 55 amino acids. P(n) = *F 0.24 *F 2) When translated in all three frames, three additional matches to BAA95569 *F are found. P(n) = 6e-06 *F >gi|7768792|dbj|BAA95569.1| RNase H and integrase-like protein Bombyx mori *F Score = 44.3 bits (103), Expect(2) = 6e-06 *F Identities = 22/47 (46%), Positives = 29/47 (61%) *F Frame = \+2 *F Query: 752 VQLEIYYSLDTSSCAMCLTNLMSRRATPKKIFSDNGTNFNGEGGEGR 892 *F \+ LEI SL+T S M L \+++RR P \+I+SDNGTN G E R *F Sbjct: 633 IHLEIAASLNTHSAVMALRRMIARRGCPTQIWSDNGTNLKGADRELR 679 *F Score = 39.7 bits (91), Expect = 0.13 *F Identities = 16/33 (48%), Positives = 25/33 (75%) *F Frame = \+2 *F Query: 179 FLMYRA*EIMETTSANQWRWVPTKLNVADLATK 277 *F \++ \+R I \++++ N+WRWVPTK NVAD AT+ *F Sbjct: 279 YVAHRLAAIEDSSTVNEWRWVPTKHNVADDATR 311 *F Score = 29.3 bits (64), Expect(2) = 6e-06 *F Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 7/42 (16%) *F Frame = \+1 *F Query: 616 VRKCCQRCKNNSA-----KMSPLPPARVA--MKPFTYTGVDF 720 *F V KC Q C+ \+ \+ LPP R+A \+PFT+TGVD+ *F Sbjct: 565 VNKCLQ-CRMKTQIPSYPRTGDLPPCRLAHHKRPFTFTGVDY 605 *F It's also repetitive in Anopheles # *U FBrf0161540 *a Peifer *b M. *t 2003.9.5 *T personal communication to FlyBase *u FlyBase error report for CG40290 on Fri Sep 5 07:25:50 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 5 Sep 2003 07:25:50 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG40290 on Fri Sep 5 07:25:50 2003 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG40290 *F Release: 3 *F Missed gene *F Comments: While this CG does not match known transposons, it is repetitive in *F the genome, with 7 blast matches with P(n) < e-10 # *U FBrf0161541 *a Peifer *b M. *t 2003.9.5 *T personal communication to FlyBase *u FlyBase error report for CG30442 on Fri Sep 5 07:36:19 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 5 Sep 2003 07:36:19 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG30442 on Fri Sep 5 07:36:19 2003 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG30442 *F Release: 3 *F Missed gene *F Comments: I think this CG is a possible retrotransposon. *F 1) It is repetitive in genome, with 8 blast matches with P(n) < e-10. *F 2) It has a weak blast match to CAC59744 putative retrovirus-like env *F glycoprotein Drosophila virilis 33% identity over 65 of 126 amino acids. *F P(n) = 0.046 *F >gi|15384852|emb|CAC59744.1| putative retrovirus-like env glycoprotein *F Drosophila virilis *F Length = 488 *F Score = 42.7 bits (99), Expect = 0.001 *F Identities = 22/65 (33%), Positives = 42/65 (64%), Gaps = 7/65 (10%) *F Query: 62 SINWLGSAWKWIAGNPDAADWNTILATQKVPLKNSDQQLRINS-------RLFDATHESI 114 *F S+++LG+A K \+AG PDA+D+ I T+ \++ \+ \+Q++INS RL D \++ I *F Sbjct: 102 SLDFLGTALKVVAGTPDASDFLRIRVTETQLVEANSKQIQINSETQKQINRLTDTINKII 161 *F Query: 115 TNKRG 119 *F \++++G *F Sbjct: 162 SSRKG 166 # *U FBrf0161542 *a Peifer *b M. *t 2003.9.5 *T personal communication to FlyBase *u FlyBase error report for CG1294 on Fri Sep 5 07:38:40 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 5 Sep 2003 07:38:40 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: peifer@unc.edu *F Subject: FlyBase error report for CG1294 on Fri Sep 5 07:38:40 2003 *F Error report from Mark Peifer (peifer@unc.edu) *F Gene or accession: CG1294 *F Release: 3 *F Missed gene *F Comments: I think this CG may be a transposon *F It is repetitive in the genome, with 24 blast matches with P(n) < e-10. *F The best blast protein match is AAB66824 Tel1, a transposable element of D. *F virilis. *F >gi|2330989|gb|AAB66824.1| Tel1 Drosophila virilis *F Length = 588 *F Score = 43.9 bits (102), Expect = 4e-04 *F Identities = 38/141 (26%), Positives = 56/141 (39%), Gaps = 29/141 (20%) *F Query: 2 LRELLDKFNDHIRALNKMGSTEQISGCT---------STCSNAGSGESGEMG-----RAF 47 *F LRE DK N H+RAL MGS EQI+GC \+ \+ A \+ \+ F *F Sbjct: 249 LREFSDKLNSHLRALKSMGSVEQIAGCVIVHTLLQKLDSVTQASWEDDAPLDVIPSCERF 308 *F Query: 48 RGPSVQKCN-FDVGIHGIASGPAVKDAGEHGVRHGKLNACNRTPALCRSDLLATSLSPAT 106 *F \++C \+ H A G++ N+ RT \+ R+ \+ *F Sbjct: 309 TTFIERRCQRLENADHATAMYTPSSQVGQN-------NSSRRTFVVTRN-------GTSA 354 *F Query: 107 CAFCHASGHSIYHFQSFKSLN 127 *F C FC \+GHSIY F \+L+ *F Sbjct: 355 CVFCEVAGHSIYKCLQFANLS 375 # *U FBrf0161563 *a Roote *b J. *t 2003.9.18 *T personal communication to FlyBase *u *F Date: Thu, 18 Sep 2003 11:32:13 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{betaTub85D-FLP}1 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote, University of Cambridge (9/03). *F P{betaTub85D-FLP}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion generated in the laboratory of Gary Struhl. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161564 *a Kaufman *b T. *t 2003.9.18 *T personal communication to FlyBase *u *F Date: Thu, 18 Sep 2003 09:25:01 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GAL4::VP16-nos.UTR}MVD2 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (9/03). *F P{GAL4::VP16-nos.UTR}MVD2 is a homozygous and hemizygous viable and *F fertile, X chromosome insertion generated in the laboratory of Ruth *F Lehmann. The construction and transformation of P{GAL4::VP16-nos.UTR} were *F described in Van Doren et al., Current Biology 8: 243--246, 1998 (FBrf0100715). *F ________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161565 *a Bossing *b T. *t 2003.9.18 *T personal communication to FlyBase *u *F Date: Thu, 18 Sep 2003 10:04:34 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GawB}43 *F Torsten Bossing of the Wellcome/Cancer Research UK Institute confirmed that *F the P construct in P{GAL4}43 (FBti0024397) is P{GawB}. *F The insertion is homozygous viable and fertile. It maps to chromosome 2 *F based on its segregation from CyO in the cross w1118; *F P{w+mW.hs=GawB}43/CyO, P{ry+t7.2=sevRas1.V12}FK1 females x Canton S males. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161566 *a Kaufman *b T. *t 2003.9.18 *T personal communication to FlyBase *u *F Date: Thu, 18 Sep 2003 17:31:57 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Kaufman insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (9/03). *F P{UAS-pb.A}49.1, P{UAS-lab.M}X2, P{UAS-Antp.Mb}W1 and P{UAS-Scr.K}EE2 are *F homozygous viable and fertile, second chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161567 *a LaJeunesse *b D. *t 2003.9.12 *T personal communication to FlyBase *u *F Personal communication from: D. LaJeunesse *F To: Bloomington Drosophila Stock Center *F Subject: EYFP constructs *F Dennis LaJeunesse, U. North Carolina at Greensboro, described three *F constructs in a poster at the last fly meeting: *F Lake, J., Na, C., Buckner, S., LaJeunesse, D. *F Organization of intracellular membrane bound compartments. *F A. Dros. Res. Conf. 44 2003 :259A *F FBrf0155063 *F The constructs encode EYFP with sequences that target the protein to *F the mitochondria, the Golgi or the endoplasmic reticulum. The 'pSpSq' *F sequence in the vectors is the promoter from the spaghetti squash (sqh) *F gene, which is expressed ubiquitously. *F [FlyBase curator comment: Diagrams of the 3 constructs are archived at *F FlyBase and can be obtained by e-mailing: flybase-offprint@morgan.harvard.edu] # *U FBrf0161568 *a LaJeunesse *b D. *t 2003.9.19 *T personal communication to FlyBase *u *F Date: Fri, 19 Sep 2003 09:54:55 \-0500 *F To: gm119@gen.cam.ac.uk *F From: Kevin Cook *F Subject: LaJeunesse insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Dennis LaJeunesse, University of North Carolina at *F Greensboro (8/03). *F P{sqh-EYFP-Mito}3 and P{sqh-EYFP-Golgi}3 are homozygous viable and fertile, *F third chromosome insertions. *F P{sqh-EYFP-ER}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161569 *a Kaufman *b T. *t 2003.9.19 *T personal communication to FlyBase *u *F Date: Fri, 19 Sep 2003 10:40:36 \-0500 *F To: gm119@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-Dfd.B}W4 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (9/03). *F P{UAS-Dfd.B}W4 is a homozygous viable and fertile, second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161570 *a Luschnig *b S. *t 2003.9.19 *T personal communication to FlyBase *u *F Date: Fri, 19 Sep 2003 16:14:03 \-0700 *F From: Stefan Luschnig *F To: Sima Misra *F Subject: Re: existence-uncertain genes *F ... *F > *F > *F > > Dear Sima, *F > > *F > > thanks for the reply. *F > > I'll compile a list of the existence-uncertain CGs that I find as genes *F > > that change in various array experiments. I am also in the process *F > > of doing a relatively large number of in situ's on these genes; do you *F > > want to wait until I have that data , or would you want me to send the *F > > information as I have it right now ? *F > > *F > > In any case, I just found the following three uncertain genes to show *F > > reduced transcript levels in ribbon (rib) mutant embryos compared to WT *F > > embryos. If I look more carefully through all my experiments, there will *F > > certainly be more. I'll keep in touch and let you know when I have all the *F > > info together. *F > > *F > > CG13064 *F > > CG10394 *F > > CG17588 *F > > *F > > cheers *F > > Stefan *F > > *F > > *F > > >Dear Stefan, *F > > > *F > > >Thank you very much for your message. We did detail our methods in *F > > >re-annotating the genome to create Release 3.1, both in this paper *F > > > *F > > >http://genomebiology.com/2002/3/12/research/0083 *F > > > *F > > >and at *F > > > *F > > >http://www.fruitfly.org/annot/reannot-guidelines.html *F > > > *F > > >Basically, if a gene encoded a small protein (<50aa) we required there to *F > > >be experimental evidence in FlyBase for its existence. However, since *F > > >these checks were done by hand, sometimes a curator missed a piece of *F > > >experimental evidence, and a gene was mistakenly deleted. It sounds as *F > > >though CG13064 is a case of such a gene that should be reinstated in a *F > > >future release. *F > > > *F > > > > I'd be happy to compile a list of the existence-uncertain genes *F > that I've *F > > > > come across in my array experiments. *F > > > *F > > >That would be wonderful. If you sent such data to *F > > >flybase-updates@morgan.harvard.edu, we could curate it as a personal *F > > >communication from you, and then we could reinstate the genes based on *F > > >attributable evidence. *F > > > *F > > >Thanks very much, *F > > > *F > > >Sima Misra *F > > >FlyBase BDGP *F > > > *F > > > > ___________________________________________________________ *F > > > > Stefan Luschnig, Ph.D. *F > > > > Department of Biochemistry/HHMI *F > > > > 279 Campus Drive, Beckman Center Rm. B453 *F > > > > Stanford University School of Medicine *F > > > > Stanford, CA 94305 *F > > > > USA *F > > > > *F > > > > lab phone (650)-723-5872 *F > > > > Fax (650)-723-6783 *F > > > > E-mail luschnig@stanford.edu *F > > > > ____________________________________________________________ *F > > > > *F > > *F > > # *U FBrf0161571 *a Nairz *b K. *t 2003.9.16 *T personal communication to FlyBase *u *F To: hafen@zool.unizh.ch, nairz@zool.unizh.ch *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 16 Sep 2003 14:42:46 \+0100 *F Dear Drs. Hafen and Nairz, *F I am curating your paper for FlyBase: *F Rintelen et al., 2003, Development 130(15): 3479--3490 *F .. *F 1. sevE(f4)-lacZ *F could you tell me some more details about this unpublished construct so *F that I can get the details of it correct in the database (I would *F record this information as a personal communication from you to *F FlyBase). *F It would be useful to know: *F a. is it an enhancer trap or promoter fusion construct *F b. which gene is the enhancer trap in or which promoter drives *F expression of lacZ in the construct *F c. which vector is it in (this helps with working out the marker genes *F e.g. w or ry) *F .. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Tue, 16 Sep 2003 16:32:44 \+0200 *F Subject: Re: FlyBase query *F From: Knud Nairz *F To: 'Gillian Millburn (Genetics)' *F Dear Gillian, *F .. *F > *F > 5. sevE(f4)-lacZ *F > *F > could you tell me some more details about this unpublished construct so *F > that I can get the details of it correct in the database (I would *F > record this information as a personal communication from you to *F > FlyBase). *F > *F > It would be useful to know: *F > *F > a. is it an enhancer trap or promoter fusion construct *F > b. which gene is the enhancer trap in or which promoter drives *F > expression of lacZ in the construct *F > c. which vector is it in (this helps with working out the marker genes *F > e.g. w or ry) *F It is an vitro construct \- a sevenless enhancer fragment was cloned into the *F reporter plasmid pX27 (Segalat et al, 1994). *F .. *F Sincerely, *F Knud # *U FBrf0161572 *a Cooley *b L. *t 2003.9.22 *T personal communication to FlyBase *u *F Date: Mon, 22 Sep 2003 13:12:02 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Act5C.T:GFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Lynn Cooley, Yale University (9/03). *F P{UAS-Act5C.T:GFP}127.1.1 is a homozygous and hemizygous viable and *F fertile, X chromosome insertion. *F P{UAS-Act5C.T:GFP}127.37.2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-Act5C.T:GFP}127.18.4 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161589 *a Deal *b J. *c M. *d Deal-Herr *e K. *f Cook *t 2003.7.22 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jul 22 20:52:17 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC37 *F Isolation and characterization of Df(2L)BSC37 *F Jennifer Deal, Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC37 was isolated as a P transposase-induced male recombination *F event involving P{PZ}aop03953a and P{EP}EP2232. The deletion was isolated *F as a net+-cn+ recombinant chromosome from the cross net1 b1 cn1 *F sp1 females x P{PZ}aop03953a cn1/net1 P{EP}EP2232; TMS, *F P{Delta2-3}99B/+ males. The deficiency chromosome retains the miniwhite *F marker from P{EP}EP2232. Polytene chromosome squashes showed the *F breakpoints 22D1-3;22F1-2 (distal break between 22D1 and the 22D3,4 *F doublet; proximal break within the 22F1,2 doublet). Df(2L)BSC37 failed to *F complement Rab5k08232. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161590 *a Bloomington Drosophila Stock Center *b ?. *t 2003.8.1 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Aug 01 20:53:17 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Df(2R)Px1 *F Hi Rachel-- *F Could you add the following comment or something similar to the entry for *F Df(2R)Px1 (FBab0002026), please? *F 'Complementation tests reported for Df(2R)Px1 may have used a Df(2R)Px2 *F stock. The Mid-America Stock Center stock 1779 and the Bloomington Stock *F Center stock 750, which were labeled Df(2R)Px1, were in fact Df(2R)Px2 *F stocks. The mix-up predates 1991.' *F Darin Coulson and John Roote at Cambridge discovered the problem. *F Thanks! *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161591 *a Brower *b D. *t 2003.8.5 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Tue Aug 05 00:46:53 2003 *F Subject: P{white-un4}BE1305 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Danny Brower, University of Arizona *F To: Bloomington Drosophila Stock Center *F Subject: P{white-un4}BE1305 *F Dated: December 1999 *F P{white-un4}BE1305 is inserted within 200 kb of mew. *F Stock Center Comment: Based on the location of mew in release 3.1 we *F estimate the cytology for this insertion as 11D8-12A2. # *U FBrf0161592 *a Zhang *b N. *t 1994.9 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Mon Aug 04 23:58:52 2003 *F Subject: In(2R)46C *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Nian Zhang, Roche Research Center *F To: Bloomington Drosophila Stock Center *F Subject: Df(2R)44CE *F Dated: September 1994 *F Background: Nian reported a previously unidentified inversion on this *F deficiency chromosome. We have named it In(2R)46C. *F Information communicated: *F The Df(2R)44CE chromosome carries an inversion with the breakpoints 46C;49B. # *U FBrf0161593 *a Franklin-Dumont *b T. *t 2003.7.23 *T personal communication to FlyBase *u *F From gopher@rail.bio.indiana.edu Wed Jul 23 18:22:18 2003 *F To: flybase-help@morgan.harvard.edu *F Reply-to: dumont@mail.med.upenn.edu *F Subject: Feedback about EP(3)3236 *F comments: To whom it may concern: *F I wanted to let you know of some recent results I got that calls into *F question the reported insertion site of EP(3)3236. I am in the process of *F mapping a mutation in the 95C region, so I ordered available P elements in *F this region. To confirm their insertion sites, I designed primers that lie *F w/in about 500bp of the reported insertion site. I purified genomic DNA from *F this EP line and used my flanking primers (see below) along with primers *F specific to the 5' and 3' P ends used for inverse PCR (Plac1 and Pry4, *F respectively). I have used this to successfully confirm the insertion sites *F of the following: EP(3)3135, EP(3)3572, EP(3)3285, EP(3)3283, and *F P{SUPor-P}KG02255. I was unable to amplify on either side of the 3236 EP *F element, though could generate a band using just the genomic primers with each *F other (the genomic DNA was from a heterozygote). Please note that the forward *F primer was purposely designed to fall outside of the opus{}1412 repeat region. *F I got negative results in both possible orientations of the P element. *F Genomic primers: *F (forward) 3236F0 \- AGTTAATGCAGCTAACCGC *F (reverse) 3236R0 \- ACTCGCAATCTGCATATTCC *F When I do a pattern search using the flanking sequence reported for this *F element, the 27-mer can be found at numerous sites in the genome, including 5 *F different sites in the 90s region of 3R. While it's possible the stock I *F received was mislabeled or some other type explanation, I thought I should *F caution you about positioning this EP element at 95C6 (as in the list of *F 'insertions by map position'). *F Let me know if any other information would be helpful. *F Thanks, *F Tina *F mailto: flybase-help@morgan.harvard.edu *F realname: Tina Franklin-Dumont # *U FBrf0161594 *a Steel *b F. *t 2003.8.7 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Thu Aug 07 20:17:42 2003 *F Subject: T(2;3)rdgCco6 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Fintan Steel, University of *F Notre Dame *F To: Bloomington Drosophila Stock Center *F Subject: T(2;3)rdgCco6 *F Dated: May 1990 *F Information communicated: *F The breakpoints of T(2;3)rdgCco6 are 35A;77B1, with the *F caveat that *F the 35A breakpoint is less certain than the 77B1 breakpoint. # *U FBrf0161595 *a Gavis *b E. *t 2003.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Aug 15 13:30:11 2003 *F To: rd120@gen.cam.ac.uk *F Subject: P{Hsp83-MCP-GFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Liz Gavis, Princeton University (8/03). *F P{Hsp83-MCP-GFP}11 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{Hsp83-MCP-GFP}3 is a homozygous viable and fertile, second chromosome *F insertion. *F P{Hsp83-MCP-GFP}5A is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161596 *a Kaufman *b T. *t 2003.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Aug 22 17:19:51 2003 *F To: rd120@gen.cam.ac.uk *F Subject: P{UASp-GFP-Cnn1} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (8/03). *F P{UASp-GFP-Cnn1}26-1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UASp-GFP-Cnn1}29-3 is a homozygous viable and fertile third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161597 *a Kaufman *b T. *t 2003.8 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Aug 21 17:01:38 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UASp-GFPS65C-alphaTub84B}3 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (8/03). *F P{UASp-GFPS65C-alphaTub84B}3 is a homozygous viable and fertile, third *F chromosome insertion which originated from the work of Grieder et al. *F (Development 2000 127:4253--4264; FBrf0129835). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161600 *a Bloomington Drosophila Stock Center *b ?. *t 2003.8.29 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Fri Aug 29 01:24:36 2003 *F Subject: FBti0024396 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: FBti0024396 *F P{GAL4}C147 is a synonym for P{GawB}C147, an insertion generated *F by Lynn Manseau, University of Arizona, and colleagues (the *F screen is described in FBrf0095642). # *U FBrf0161601 *a Cook *b K. *t 2003.8.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Aug 29 14:24:57 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(1)w-194A cytology *F I analyzed polytene chromosome squashes of Df(1)w-194A (FBab0014444) and *F found the following. The distal breakpoint is either in the interband *F between 3A1 and 3A2 or in the distal part of 3A2. The proximal breakpoint *F lies between the 3C2,3 doublet and the 3C5,6 doublet. Consequently, the *F breakpoints are 3A1-2;3C3-5. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161602 *a Deal-Herr *b M. *c K. *d Cook *t 2003.8.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Aug 29 20:54:37 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2R)BSC39 *F Isolation and Characterization of Df(2R)BSC39 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC39 was isolated as a P transposase-induced male recombination *F event involving P{PZ}Ef1alpha48D01275 and P{GT1}Rep1BG01033. The *F deletion was isolated as a cn1-bw1 recombinant chromosome from the *F cross cn1 bw1 females X cn1 *F P{PZ}Ef1alpha48D01275/P{GT1}Rep1BG01033 bw1 sp1; TMS, Sb1 *F P{ry+t7.2=Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 48C5-D1;48D5-E1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161603 *a Deal-Herr *b M. *c K. *d Cook *t 2003.8.29 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Fri Aug 29 20:57:30 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2R)BSC40 *F Isolation and Characterization of Df(2R)BSC40 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC40 was isolated as a P transposase-induced male recombination *F event involving P{GT1}Rep1BG01033 and P{PZ}Cct506444. The deletion was *F isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 *F females x cn1 P{PZ}Cct506444/ P{GT1}Rep1BG01033 bw1 sp1; TMS, *F Sb1 P{ry+t7.2=Delta2-3}99B/+ males. Polytene chromosome squashes showed *F the breakpoints 48E1-2;48E2-10. Df(2R)BSC40 failed to complement *F SmD3k09029 and jebk05644. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161604 *a Montana *b E. *t 2003.9.7 *T personal communication to FlyBase *u *F From rd120@gen.cam.ac.uk Tue Mar 05 15:53:46 2002 *F To: emontana@mit.edu *F Subject: Helping FlyBase: CSH-37 *F Cc: rd120@gen.cam.ac.uk *F Dear Enrico, *F We are currently curating the abstracts for the 2001 CSH Neurobiology *F of Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Genetic and molecular identification of novel syntaxin interacting *F proteins'. *F You mention several genes that are new to FlyBase; SIP1, SIP2 and SIP3, *F a (un-named) novel decarboxylase and an (un-named) homolog of SNAP-29. *F Do you know which of the Genome Project CG annotations any of these *F genes corresponds to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From emontana@mit.edu Sun Sep 07 19:24:57 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-37 *F I looked up the sequences in Flybase, and these are the corresponding gene *F records. I'm sorry this took a while, but I had not worked on these *F proteins for some time and had to find all the records. *F SIP1 = CG30173 *F SIP2 = CG13164 *F SIP3 = CG15468 *F The decarboxylase is CG1486 *F SNAP-29 homolog = CG11173 (ubisnap) *F I hope this helps. Please feel free to email me with further *F questions. Thank you for all the effort and time that goes into Flybase *F and the invaluable resources it provides. *F Rico # *U FBrf0161605 *a Cooley *b L. *c X. *d Morin *e W. *f Chia *t 2002.10-2003.5 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Mon Sep 08 22:16:44 2003 *F Subject: GFP Protein Trap Insertion Lines *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Lynn Cooley, Yale School of Medicine, *F Xavier Morin and William Chia, King's College London *F To: Bloomington Drosophila Stock Center *F Dated: October 2002 through May 2003 *F Subject: GFP Protein Trap Insertion Lines *F Background: The information included here was provided by Lynn, Bill *F or Xavier in connection with stocks donated to the stock center, or *F it was extracted from http://flytrap.med.yale.edu/. These lines were *F generated in the Chia lab, as described in Morin et al. 2001. Some *F sequence data was generated in the Chia lab and some in the Cooley *F lab. Expression patterns were determined by the Cooley group. Images *F of the GFP expression patterns are at http://flytrap.med.yale.edu/. *F Questions can be directed to Lynn Cooley (lynn.cooley@yale.edu) *F and/or Bill Chia (william.chia@kcl.ac.uk). *F 1. P{PTT-GC}ATPalphaG00109 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 93B2 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | membrane; *F follicle cell | cytoplasm *F 2. P{PTT-GA}AsphZCL1605 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 52E4--5 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | cytoplasm (faint); *F nurse cell | cytoplasm (faint) *F 3. P{PTT-GC}CG1640G00282 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 11F1 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | cytoplasm; *F nurse cell | cytoplasm (punctate); nurse cell | nucleus; *F oocyte | cytoplasm *F 4. P{PTT-GC}CG3735ZCL1614 *F sequence data: *F GAACGTGCCTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGAC *F GGGACCACCTTATGTTATTTCATCATGCCGAGTATCTTTCGCACACACG *F TGTGGATCAACAAACATCTGGATTGGTGAAGGAATCCGATACACAGAC *F ATATCGATAGCTCCTAGAGATGGCGTGTGGTGAATTTAAAGCCGGGAT *F ATATTTTTTATATACATACTTTTCAAATCGCGCGCCCTCTTCATAATTC *F ACCTCCACCACACCACGTTTCGTAGTTGCTCTTTCGCTGTCTCCCACCC *F GCTCTCCGCAACACATTCACCTTTTGTTCGACGACCTTGGAGCGACTGT *F CGTTAGTTCCGCGCGATTCGGTTCGCTCAAATGGTTCCGAGTGGTTCAT *F TTCGTCTCAATAGAAATTAGTAATAAATATTTGTATGTACAATTTATTT *F GCTCCAATATATTTGTATATATTTCCCTCACAGCTATATTTATTCTAATT *F TAATATTATGACTTTTTAAGGTAATTTTTTGTGACCTGTTCGGAGTGAT *F TAGCGTTACACNCTNNNNTCNGCTCCNCCCTCNANCGNNCN *F estimated cytological location: 60A12 *F viable: yes *F fertile: yes *F GFP expression pattern: no ovary expression *F 5. P{PTT-GC}CG6084G00112 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 68C15 *F viable: yes *F fertile: yes *F GFP expression pattern: nurse cell | nucleus; *F follicle cell | nucleus; *F oocyte associated follicle cell | nucleus *F 6. P{PTT-GA}CG6416ZCL0663 *F sequence data: *F ACACGAAATGAGAAACACACACAATTCGAACATACGATTGAACACGA *F AAACCAGTCCATAGATGTTGTACGTACTACTTACTACTGCCTACTGCCG *F GGATCACTCTCGGCATGGACGAGCTGTACAAGTCGACCTCGAGGTAAG *F TTATTTGAACAATGGCATCAAATGCCTTCATCATCACTACCCTTTAGCC *F CTTAAGACCCCACAATGACCTTACCCACTCAGAGAAAAAAGTAAATAT *F GAAAGCCCATTTGAACTTCTCTGCAGCCAAGCTTTGCGTACTCGCAAA *F TTATTAAAAATAAAACTTTAAAAATAATTTCGTCTAATTAATATTATGA *F GTTAATTCAAACCCCACGGACCATTGCTAAGGANNNNNNNNNNNNNN *F NNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNCNNNNNNNNTNNNN *F NNNNNTNGNNNNNNNNGNNNNCNNNCGNNNNNNNNNNGGNNNNNNN *F CCNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN *F NNNCNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNN *F NNNNCGGNNNNNNNNNNNNNNNNNNNNNNGNGNNNNNGCGNNCCNN *F NNNNNCNNNNNNNNCCNNNNNNNNNNNNNNNCNNNNNNNNCNNNNN *F CNNNNNNNNCNTNNNNNNCNNNNCNNNNCNCNNCNNNNNNNNNNNN *F NNNNNNNNNNNCNCNNNCNNNNNNNNNNNNNGN *F estimated cytological location: 66D8--9 *F viable: yes *F fertile: yes *F GFP expression pattern: ovarian sheath muscle *F 7. P{PTT-GB}CG8029ZCL0366 *F sequence data: *F CTAGAAAAAAATTATTCGACTCTGAGAGCATTTCGTAAAGCCTAATTG *F GATATTTGCACCAGGAACTGACATTAACTGGGTTTCTTTTCAAAAGTA *F ATACTTATAAGAAAGTATAGNTTGTCAAATAAGGTTCATATCTTCGGT *F GTCCAACTAGCGCAGTCACACACCTTTCCAGCTGGAAACTGTCAACGA *F AACCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTCGACCTCGAG *F GTAAGTTATTTGAACAATGGCATCAAATGCCTTCATCATCACTACCCTT *F TAGCCCTTAAGACCCCACAATGACCTTACCCACTCAGAGAAAAAAGTA *F AATATGAAAGCCCATTTGAACTTCTCTGCAGCCAAGCTTTGCGTACTC *F GCAAATTATTAAAAATAAAACTTTAAAAATAATTTCGNCTAATTAATA *F TTATGAGTTAATTCAAACCCCACGGACATGCTAAGGGGGNNCCCNNCC *F CCCCCGGGNNGGGNNGCGGGNNGGCNGNCNCNNNGGGCNNCNCNCG *F TCNGCCCCNNCNNNNGGCTNNCCCCGCCTGGGNTNCCGGCNCCACNCC *F NGCCCCTGTNCCGNNCCCTTNNTCNTTGTCCCNNNNNNTNNCNCNGTC *F CNCGNCNNNNCCGNNNNCNGGTNNNNCNNGNCCTNCNGCTCTNCGNN *F NGTNNNNCCCNNTTNCCCCCCCNNCCGNCNTNNNTTNNCGTCNCCGCG *F NNTGCCCNGGGCGTNCCNNCNCNTATGCGGTCCNCCCCCGCCNGNCTC *F CCCTNNNTGCT *F estimated cytological location: 45B4 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | nucleus; *F nurse cell | nucleus *F 8. P{PTT-GA}CG8443G00271 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 52F6--7 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | cytoplasm; *F nurse cell | cytoplasm (punctate); oocyte | cytoplasm *F 9. P{PTT-GC}CG10997G00264 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 12C2 *F viable: unknown *F fertile: unknown *F GFP expression pattern: nurse cell | cytoplasm *F 10. P{PTT-GA}CG14648ZCL3169 *F sequence data: *F TGCTCATACGAATACATATTAATTGAGAAATTCGCGAGTCGATGCGCT *F CATCGCCCAGAAATGTGTGCGTGCGAGTGTGTGCGTGTCGAAGCGTTG *F CCAACTTATTGGTCATGCGTTAATATCGGGATATGGGTTCTTAATTTGT *F ATTTCTCATTGAACCCGCCAAGAAAGACGGCCGAAATAGTTTTAGCGG *F CCAATTCATACTTCAGTTTCAATTCAACAAGCGCACACACATGCATGTT *F GACGTGTGTTCTTTAACCAGCACAGGTGGCAACCCTGCCGTCCCGATT *F TTGCATGCAGATTTTTGTTTTCAATGATACGGGCCGGGATCACTCTCGG *F CATGGACGAGCTGTACAAGTCGACTCGAGGTAATTATTTGAACAATGG *F CATCAAATGCCTTCATCATCNACTACCCTTTAGCCCTTAAGACCCCACN *F ATGACCTTACCCNACTCAGAGAAAAAAGTAAATATGAAAGCCCCATTT *F GAACTTCCTCTGCAGCCAAGNTTTGCGTACTCGCTAATTATTAAAAAA *F TAAAACTTTNAAGAATAATTTCCNTCTNAATTAAATATTATGAGTTAAT *F TCAAACCCCCACNGCACNATGCCNAANGGATANTANTCCTCCCACNNC *F TTNCTACCTNCCTNAACCNTACNCTCCCGNCGCATTNATNTCNTNTNCC *F CGTNNCCCTANANGACCTATAGCCTGNCCTATNCTAANANAAAAAAN *F NTNNN *F estimated cytological location: 82B1 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | cytoplasm; *F nurse cell | cytoplasm (punctate); oocyte | cytoplasm *F 11. P{PTT-GA}CG18763ZCL0931 *F sequence data: *F GAACGTGNCTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGAC *F GGGACCACCTTATGTTATTTCATCATGATGTGGCGCATCAAAACAAAA *F ACAAACTACAGCTGATGCTGATTAGAGTTGGAGCCCGCTCTCTTCCAT *F GCTCTCTCCCTCTCTCTATCTCTCACTTGTGGGGCAGAACCCAACTCGT *F GGTGAGTTCTTCACTCCGCTTGTAGGTCACAAAAAAGTTTAAAATACG *F CCAAGAACTGAAGCGAAAATAAAACATATACTTAAGCCGTGTTTAAG *F ACACTTGCGGGATTAACATTCTGCATTTATTTATCTCATTTTATGTGCC *F CCACCGGGATATATTTTTTATATACATACTTTTCAAATCGCGCGCCCTC *F TTCATAATTCACCTCCACCACACCACGTTTCGTAGTTGCTCTTTCGCTG *F TCTCCCACCCGCTCTCCGCAACACATTCACCTTTTGTTCGACGACCTTG *F GAGCGACTGTCGTTAGTTCCGCGCGATTCGGTTCGCTCAAATGGTTCC *F GAGTGGTTCATTTCGTCTCAATAGAAATTAGTAATAAATATTTGTATGT *F ACAATTTATTTGCTCCAATATATTTGTATATATTTCCCTCACAGCTATA *F TTTATTCTAATTTAATATTATGACTTTTTAAGGTAATTTTTTGTGACCTG *F TTCGGAGTGATTTANCGTTACANCCNNNNNCNCGCCCGCNANAGNTTC *F NCNCTTTTCCCCTGCCCNNCCCGTGNGTTATNTGGNNNGNCCCNNCGC *F CC *F estimated cytological location: 86E16 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | cytoplasm; *F polar follicle cell; nurse cell | cytoplasm; *F oocyte | cytoplasm *F 12. P{PTT-GB}CG30084G00189 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 52C4--6 *F viable: yes *F fertile: yes *F GFP expression pattern: ovarian sheath muscle *F 13. P{PTT-GA}CG31363G00147 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 86E16 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | cytoplasm; *F follicle cell | membrane; border follicle cell; *F polar follicle cell; nurse cell | cytoplasm; *F nurse cell | nucleus; nurse cell | nuclear membrane; *F oocyte | vitelline membrane; oocyte | cytoplasm *F 14. P{PTT-GC}Hrb98DEZCL0588 *F sequence data: *F GCATTAGTAAAATGGTGAACTCGAACCAGAACCAGAACGGCAACTCC *F AATGGCCATGATGATGTAAGTAGTGGATATTAAGGGTCTAGCAATTTC *F CTGTAAACTGGTGTCTCGCCTTACCGGGATCACTCTCGGCATGGACGA *F GCTGTACAAGTCGACACTCGAGGTAAGTTATTTGAACAATGGCATCAA *F ATGCCTTCATCATCACTACCCTTTAGCCCTTAAGACCCCACAATGACCT *F TACCCACTCAGAGAAAAAAGTAAATATGAAAGCCCATTTGAACTTCTC *F TGCAGCCAAGCTTTGCGTACTCGCAAATTATTAAAAATAAAACTTTAA *F AAATAATTTCGTCTAATTAATATTATGAGTTAATTCAAACCCCACGACC *F ATGGCTTAAGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN *F NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN *F NNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNGNNNNNGNNN *F NNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNG *F NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNN *F NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNCCCN *F NNNNNNCCNNNNNNNNNNNNNNNNCNNNNNNCNGNNNNNNGNNNNN *F NCNNNNCNNCNNNCNCNNNNNNNCNNCCNNNCNNNNCNNNNNNNNN *F NNNCCCCNCCNNNGNGNNNNNNNNNN *F estimated cytological location: 98D6 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | nucleus; *F nurse cell | nucleus *F 15. P{PTT-GB}IlkZCL3111 *F sequence data: *F GAACGTGCCTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGAC *F GGGACCACCTTATGTTATTTCATCATGATACGTATACTTATATATTTGA *F TTCGTCTGTTTACACAACACAAGAATAAATCATGACGTCACTTAGACT *F TGAAAGACCCTCGAAATCTTGGGCGTATTTAGGCGATGACTAAGTAAA *F CGTTCCTGGTAGAGGGGGTTGCTACTTGAGGGCTTAACTTTCTAAGTG *F CACTATAAAGCAACTAGAAAGTGATGGAGTTAGTTGCCTCTATTGGAT *F TTACCCCAAATTGTTGTCGTGCTCCGTTTCATCCAACCAGAGGCGCACT *F TGAATCGAGTTGCCCTCGCGGCACCAGTGGAATATGTCCTCCATTTTTA *F CTCCTCACTCCTCTATTCCGCTCGCAGTCACTGATATTATAGGTTTTCC *F AACCTAATTTACATCTAGAAACAGATTTAAACGCTTAAAATGCGACAG *F TTGTGAGAATTCCAATTAAAATTTGTGCAGTGTGGACGTTTTAAATATT *F TAACACACTGCTCGCGTTGCCAACTGCTGCAACACATCGACAAGTGTG *F TATCGTGGTATCGATGACATACACCAATTGTGAATGTTTAAAGCAAGA *F AGAAGAAGGAATACAACGTGCACCGCAACTATTATTTAAAAACATTGT *F TGTTTACCTCATCTCTTTGTATTAATTAAACCATTACNAGCCCGAAAAT *F GAAACTCAGCACATACAACTTCTTGACCTCCGTGGCCATCAAGGGCGT *F CAAAGTGGG *F estimated cytological location: 78C2 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | nucleus; *F follicle cell | cytoplasm *F 16. P{PTT-un1}LacG00044 *F construct could be PTT-GA, PTT-GB or PTT-GC *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 49A6--7 *F viable: unknown (unverified lethal) *F fertile: unknown *F GFP expression pattern: no ovary expression *F 17. P{PTT-GB}LamCG00158 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 51B1 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | nucleus; *F nurse cell | nucleus; oocyte | nucleus *F 18. P{PTT-GC}larpZCL2726 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 98C3 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | cytoplasm; *F nurse cell | cytoplasm; oocyte | cytoplasm *F 19. P{PTT-GA}NrgG00305 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 7F3--4 *F viable: yes *F fertile: yes *F GFP expression pattern: follicle cell | cytoplasm; *F follicle cell | membrane; border cell | membrane; *F nurse cell | membrane (faint) *F 20. P{PTT-GA}Nrv2ZCL1649 *F sequence data: *F GCACGTGCACTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGA *F CGGGACCACCTTATGTTATTTCATCATGATTCAAACTATATACACTCGT *F ATACATATACAGCAGTCCAGGTCCTTTTTGCTCTCTTAGTTTCTCTCAC *F TTTTCACCCGACGTTTCAACCCCCCTGGCCGTTTTGGAAATTTGGCGCC *F TTTTTCGGCTCTCCTGGCCGAAAAAAATGTTGCATCCGTCAGATACGA *F CCTGCAACTGGTTGGCATGGAATGGGCTCGAAGGGCCAAACAATGTG *F GGCCAAAATGGGGCCAGAAGGCCGTGCTACTGCTGGGGCGGCGACAT *F GGTCCCATTTCCGCGTCTGCTGTTGTGATTTCAATGTCGCATTGGCTTT *F GACCCGGGATATATTTTTTATATACATACTTTTCAAATCGCGCGCCCTC *F TTCATAATTCACCTCCACCACACCACGTTTCGTAGTTGCTCTTTCGCTG *F TCTCCCACCCGCTCTCCGCAACACATTCACCTTTTGTTCGACGACCTTG *F GAGCGACTGTCGTTAGTTCCGCGCGATTCGGTTCGCTCAAATGGTTCC *F GAGTGGTTCATTTCGTCTCAATAGAAATTAGTAATAAATATTTGTATGT *F ACAATTTATTTGCTCCAATATATTTGTATATATTTCCCTCACAGCTATA *F TTTATTCTAATTTAATATTATGACTTTTTAAGGTAATTTTTTGTGACCTG *F TTCGGATGNAATAANACNGTTANAAANNNNNNNNNNNNNNNNNNNN *F ANNNNNN *F estimated cytological location: 27B1 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | cytoplasm (faint); *F nurse cell | cytoplasm (faint); oocyte | cytoplasm (faint) *F 21. P{PTT-GA}PdiG00198 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 71B5 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | cytoplasm; *F nurse cell | cytoplasm; oocyte | cytoplasm *F 22. P{PTT-GC}scyllaZCL0611 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 68B1--2 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | nucleus; *F follicle cell | cytoplasm *F 23. P{PTT-GC}Tm2ZCL2456 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 88E12--13 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | cytoplasm *F 24. P{PTT-GA}VhaSFDG00259 *F sequence data: Morin et al. 2001 (FBrf0141725) *F estimated cytological location: 36A6 *F viable: unknown *F fertile: unknown *F GFP expression pattern: follicle cell | cytoplasm; *F follicle cell | membrane; nurse cell | cytoplasm; *F oocyte | cytoplasm # *U FBrf0161606 *a Bloomington Drosophila Stock Center *b ?. *t 2003.9.5 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Mon Sep 08 16:54:22 2003 *F Date: Mon, 8 Sep 2003 10:50:30 \-0500 (EST) *F Subject: Re: l(3)10418 *F To: flybase-updates@morgan.harvard.edu, matthewk@indiana.edu, *F rd120@gen.cam.ac.uk *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: l(3)10418 *F The gene l(3)10418 was given a generic lethal name by Spradling *F et al. (FBrf0111489) based on the semi-lethal phenotype of the *F P{PZ}-bearing chromosome in their line 10418. The insertion has *F since been mapped to the sequence and is inserted in CG31306. In *F stock, the chromosome bearing this insertion is now viable and *F fertile. No other lethal alleles of l(3)10418 are known. I recommend *F that the misnomer l(3)10418 revert to a synonym for this gene and *F CG31306 be used as the valid symbol. *F Gene: CG31306 *F Allele: CG3130610418 *F Insertion: P{PZ}CG3130610418 # *U FBrf0161607 *a Roote *b J. *t 2003.9.11 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Thu Sep 11 11:18:09 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Df(3L)ED4079 *F Dear Rachel, *F Please could this vital piece of information be included in the entry *F for ED4079: *F Df(3L)ED4079 CB-0127-3 20950 61A5 5-SZ-3709 112410 *F 61B1 91460 *F The deletion is homozygous viable. *F But I think that's OK... There are only 4 genes in this 91460 bp region: *F Lsp1gamma lof alleles are viable and fertile *F CG13405 *F CG12483 *F Pk61C Pk61C4/Pk61C5 survive to adulthood \- flies have *F reduced heads and are male sterile. Alleles 4 and 5 are reported *F to be lof...and anyway ED4079 only removes the first 2kb of one of *F the Pk61C transcripts and misses the others by 54bp. *F Many thanks. *F John # *U FBrf0161608 *a Bienz *b M. *t 2003.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Sep 11 21:17:30 2003 *F Date: Thu, 11 Sep 2003 15:20:14 \-0500 *F To: flybase-updates@morgan.harvard.edu *F Subject: Bienz insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mariann Bienz, University of Cambridge (9/03). *F The following are homozygous viable and fertile, second chromosome insertions: *F P{UAS.LEF-1}21 *F P{UAS-Fra}2 *F P{UAS-Fra.Fbz}5 *F P{Jbz}1 *F P{dCREB-B-UAS}88 *F P{LL.lacZ}6 *F P{lexA-GAD.C}2 *F The following are homozygous viable and fertile, third chromosome insertions: *F P{UAS.LEF-1}59 *F P{UAS-Fra.Fbz}7 *F P{UAS-Jra}2 *F P{Jbz}10 *F P{dCREB-B-UAS}94 *F The following are third chromosome insertions: *F P{Cbz}4 *F P{lexA-GAD.C}3 *F P{Cbz}2 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161609 *a Bienz *b M. *t 2003.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Sep 11 21:24:46 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-Axn.GFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mariann Bienz, MRC Laboratory of Molecular Biology (9/03). *F P{UAS-Axn.GFP}2 is a second chromosome insertion. *F P{UAS-Axn.GFP}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161610 *a Myster *b S. *c M. *d Peifer *t 2003.9.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Sep 11 19:40:31 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Myster and Peifer lethals *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Steve Myster and Mark Peifer, University of North Carolina *F (6/03). *F 1. A cn1 bw1 chromosome was mutagenized with EMS and lethal mutations *F were isolated that failed to complement Df(2R)M41A8. This led to the *F identification of the following complementation groups: *F l(2)NC83 1 allele = l(2)NC83NC83 *F l(2)NC30 1 allele = l(2)NC30NC30 *F l(2)NC28 1 allele = l(2)NC28NC28 *F l(2)NC37 1 allele = l(2)NC37NC37 *F l(2)NC38 2 alleles = l(2)NC38NC38 and l(2)NC38NC173 *F l(2)NC133 1 allele = l(2)NC133NC133 *F l(2)NC110 1 allele = l(2)NC110NC110 *F l(2)NC70 1 allele = l(2)NC70NC70 *F l(2)NC19 6 alleles including l(2)NC19NC19 and l(2)NC19NC86 *F l(2)NC204 1 allele = l(2)NC204NC204 *F l(2)NC192 1 allele = l(2)NC192NC192 *F l(2)NC85 2 alleles including l(2)NC85NC85 and l(2)NC85NC117 *F l(2)NC148 1 allele = l(2)NC148NC148 *F l(2)NC139 1 allele = l(2)NC139NC139 *F l(2)NC45 1 allele = l(2)NC45NC45 *F l(2)NC130 6 alleles including l(2)NC130NC130 and l(2)NC130NC95 *F l(2)NC12 1 allele = l(2)NC12NC12 *F l(2)NC47 3 alleles including l(2)NC47NC47 and l(2)NC47NC84 *F l(2)NC114 1 allele = l(2)NC114NC114 *F l(2)NC14 4 alleles including l(2)NC14NC14 and l(2)NC14NC54 *F l(2)NC136 2 alleles including l(2)NC136NC136 which failed to *F complement P{SUPor-P}CG8426KG10496 *F 2. Additional alleles of the following complementation groups were isolated: *F l(2)IR3 5 new alleles including l(2)IR3NC1 *F l(2)41Af 1 new allele called l(2)41AfNC21 *F Nipped-A 36 new alleles including Nipped-ANC116 and Nipped-ANC186 *F 3. The following deletions were isolated from the screen: *F Df(2R)NC138 which failed to complement mutations in l(2)NC30 and l(2)NC28 *F Df(2R)NC109 which failed to complement mutations in l(2)NC37 and l(2)NC38 *F Df(2R)NC9 which failed to complement mutations in l(2)IR3, l(2)IR23 and *F l(2)NC19 *F Df(2R)NC89 which failed to complement mutations in l(2)IR23 and l(2)NC19 *F Df(2R)NC13 and Df(2R)NC228 which failed to complement mutations in *F l(2)NC83, l(2)NC30, l(2)NC28, l(2)309, l(2)NC37, l(2)NC38, l(2)NC133, *F l(2)NC110, l(2)NC70, l(2)IR3, l(2)IR23, l(2)NC19, l(2)41Af, l(2)NC204, *F Nipped-B, Nipped-A, l(2)NC192, l(2)NC85, l(2)NC148, l(2)NC139, l(2)NC45, *F l(2)NC130, l(2)NC12, l(2)NC47, l(2)NC114, l(2)NC14, l(2)NC136, vlc and Bub1 *F and deleted the CG40293, p120ctn and CG17486 sequences. *F Df(2R)NC73 which failed to complement mutations in l(2)NC192, l(2)NC85, *F l(2)NC148, l(2)NC139, l(2)NC45, l(2)NC130, l(2)NC12, l(2)NC47, l(2)NC114, *F l(2)NC14, l(2)NC136 and vlc. *F Df(2R)NC32 which failed to complement mutations in l(2)NC130, l(2)NC12 and *F l(2)NC47 *F Df(2R)NC219 which failed to complement mutations in l(2)NC47, l(2)NC114 and *F l(2)NC14 *F Df(2R)NC207 which failed to complement mutations in l(2)NC14 and l(2)NC136 *F 4. Df(2R)Dark2, Df(2R)244, Df(2R)247 and P{SUPor-P}l(2)3091 were *F isolated after mobilization of P{SUPor-P}KG01086. Df(2R)Dark2 deletes the *F CG17486 sequence. Df(2R)244 deletes the CG40293 and p120ctn *F sequences. Df(2R)247 fails to complement mutations in l(2)NC37, l(2)NC38, *F l(2)NC133, l(2)NC110, l(2)NC70, l(2)IR3, l(2)IR23, l(2)NC19, l(2)41Af and *F l(2)NC204 and deletes the CG40293 and p120ctn sequences. *F 5. Hilliker (Genetics 83: 765-782, 1976; FBrf0028786) described l(2)41Ae *F as a complex locus showing complicated interallelic complementation *F patterns. Some or all of the mutations defining the twelve most proximal *F complementation groups listed above (l(2)NC83, l(2)NC30, l(2)NC28, l(2)309, *F l(2)NC37, l(2)NC38, l(2)NC133, l(2)NC110, l(2)NC70, l(2)IR3, l(2)IR23 and *F l(2)NC19) may be alleles of l(2)41Ae *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161611 *a Bienz *b M. *t 2003.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Sep 17 01:05:47 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Bienz 95EF lethals *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mariann Bienz, MRC Laboratory of Molecular Biology (9/03). *F Imprecise w- excisions and lethal w+ hops were generated from *F P{wAR}4-95, an insertion near Apc2. Two lethal insertions in the 5' UTR *F of jaguar (P{wAR}jar2095 and P{wAR}jar1646) were isolated. Four of *F the imprecise excisions were alleles of l(3)95Ea including the allele *F l(3)95Eaw1. Two of the imprecise excisions were the deletions Df(3R)w5 *F and Df(3R)w6. Df(3R)w5 extends to the right from the P{wAR}4-95 *F insertion site. Df(3R)w6 extends to the left from the P{wAR}4-95 *F insertion site. *F In a screen for lethal mutations in 95EF, an isogenized P{neoFRT}82B *F P{piM}87E stock was treated with 45 mM EMS. Lethals were selected based on *F noncomplementation with Df(3R)crb87-4 and complementation with *F Df(3R)crb87-5. Ten complementation groups were recovered, each of which *F was represented by at least two alleles. Df(3R)w5 and Df(3R)w6 were used *F to subdivide the loci: l(3)95Ea, l(3)95Eb, l(3)95Ec, l(3)95Ed and l(3)95Ef *F fall within Df(3R)w6, and l(3)95EFa, l(3)95EFb, l(3)95EFc, l(3)95EFd and *F jar fall within Df(3R)w5. Alleles of these complementation groups include *F those listed below along with phenotypic information. *F l(3)95Ea4437 Clones with rough eyes, vein defects and wing nicks *F l(3)95Ea5383 Clones with rough eyes and missing notum bristles *F l(3)95Eb1117 Normal embryos and clones *F l(3)95Eb3627 Normal embryos and clones *F l(3)95Ec149 Normal embryos. clones with rough eyes and vein *F defects *F l(3)95Ec455 Abnormal embryos. Clones with rough eyes, wing *F nicks and vein and bristle defects. *F l(3)95Ed4378 Normal embryos. Clones with rough eyes, bristle *F defects and wing nicks *F l(3)95Ed4532 Clones with bristle defects and wing nicks. *F l(3)95Ee2153 Abnormal embryos. Clones with bristle defects. *F l(3)95Ee4454 Clones with rough eyes, wing nicks and vein and *F bristle defects *F l(3)95EFa2707 *F l(3)95EFa1103 Normal embryos and clones *F l(3)95EFb1807 *F l(3)95EFb2328 Slightly abnormal embryos. *F l(3)95EFc1665 Normal embryos and clones *F l(3)95EFc2201 *F l(3)95EFd1214 Slightly abnormal embryos *F l(3)95EFd4182 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161612 *a Bienz *b M. *t 2003.9.16 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Sep 17 01:10:47 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-pnr.H}13 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mariann Bienz, MRC Laboratory of Molecular Biology (9/03). *F P{UAS-pnr.H}13 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161613 *a Peifer *b M. *t 2003.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Sep 17 02:36:37 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Peifer insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mark Peifer, University of North Carolina (9/03). *F P{Ubi-p63E-p120ctn.DeltaC.GFP}M3 is a third chromosome insertion. *F P{Ubi-p63E-p120ctn.DeltaC.GFP}L2 is a second chromosome insertion. *F P{UAS-p120ctn.DeltaC.GFP}S2 is a homozygous viable and fertile, second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161614 *a Deal *b J. *c R. *d Andrade *e K. *f Cook *t 2003.9.17 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC41. *F From kcook@bio.indiana.edu Wed Sep 17 21:03:45 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2L)BSC41 *F Cc: jedeal@sunflower.bio.indiana.edu, Rachel Andrade , *F medeal@bio.indiana.edu *F Isolation and characterization of Df(2L)BSC41 *F Jennifer Deal, Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC41 was isolated as a P transposase-induced male recombination *F event involving P{lacW}l(2)k05404k05404 and P{EP}EP946. The deletion was *F isolated as a dpov1-cn1 recombinant chromosome from the cross dpov1 *F cn1 bw1 females x dpov1 P{lacW}l(2)k05404k05404/P{EP}EP946 cn1; *F TMS, P{Delta2-3}99B/+ males. The deletion chromosome retained one or both *F mini-white markers from the P elements used in the screen. Polytene *F chromosome squashes showed the breakpoints 28A4-B1;28D3-9. Df(2L)BSC41 *F failed to complement LanB1KG03456 and mts02496. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0161615 *a Laing *b L. *t 2003.8.25 *T personal communication to FlyBase *u *F Archived. # *U FBrf0161616 *a Bloomington Drosophila Stock Center *b ?. *t 2003.9.17 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Sep 17 01:34:55 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Wei and Brill lethals *F Hi Rachel-- *F I received some stocks from an EMS screen done in the lab of Julie Brill by *F Ho-Chun Wei. The work will be described in an article in press in the *F journal Genome. *F Here are the mutations and Dfs I have: *F Glut1L1H *F Glut117J *F Klp61F6D283 *F l(3)L3DL3D *F l(3)8E2768E276 *F l(3)8E276L14C *F l(3)4106L4C *F l(3)93589358 *F l(3)B197B197 *F l(3)B197L18C *F l(3)L3FL3F *F mtacp11303 *F l(2)026407E130 *F l(3)L1ML1M *F Df(3L)3C7 *F Df(3L)10F125 *F Df(3L)4231 *F Df(3L)L12C *F Thanks! *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0166451 *a Boundy-Mills *b K. *t 2003.12.1 *T personal communication to FlyBase *u *F Personal communication to FlyBase from Kyria Boundy-Mills, 1 December 2003 *F *F Phaff Yeast Culture Collection, University of California Davis *F *F Yeasts associated with Drosophila species. *F *F Information from http://www.phaffcollection.org/module/collection/ *F parsed by: *F *F Kyria Boundy-Mills, Curator *F Phaff Yeast Culture Collection *F Food Science and Technology *F University of California Davis *F One Shields Ave *F Davis, CA 95616 *F December 1 2003. *F *F 51-227 Aciculoconidium aculeatum 'Near Tioga Pass, 9000 ft., Yosemite National Park, 08/51' California Isolated from Drosophila occidentalis *F 73-122 Candida boidinii Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders).' *F 57-94 Candida catenulata 'Wild Rose Canyon, Panamint Mtns. 06/57' Isolated from Drosophila pseudoobscura *F 57-92 Candida catenulata 'Wild Rose Canyon, Panamint Mtns. 06/57' Isolated from Drosophila pseudoobscura *F 57-95 Candida catenulata 'Wild Rose Canyon, Panamint Mnts, southern Sierra' California tree exudate?Ex Drosophila pseudoobscura *F 57-98 Candida catenulata 'Wild Rose Canyon, Panamint Mnts.,Southern Calif.' California Ex Drosophila pseudoobscura *F 51-163 Candida catenulata? 'Aspen Valley, Yosemite area, California 07/51' Isolated from Drosophila pinicola *F 51-156 Candida catenulata? 'Aspen Valley, Yosemite area, California, 07/51' Isolated from Drosophila occidentalis *F 72-163 Candida diversa 'Kipuka Ki in Hawaiian Volcanoes Natl. Park, Hawaii.' Isolated 3/72 from wet brown flux of Acacia koa with Drosophila hawaiiensis and honey bees. *F 50-5 Candida famata 'Berkeley, California' Isolated from Drosophila sp. in a vinegar factory *F 61-211 Candida guilliermondii 'Gualala River, pacific coast, Northern Calif.' California Drosophila pseudoobscura *F 56-118 Candida krusei 'Andrews Ranch, Fresno, CA. 09/56' California Isolated from Drosophila melanogaster from a calimyrna fig *F 61-287 Candida krusei 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila pseudoobscura *F 61-551 Candida krusei 'Fruit orchard near Winters, Nov. 1961' Isolated from Drosophila melanogaster *F 61-528 Candida krusei 'Fruit orchard near Winters, Oct. 1961' Isolated from Drosophila melangaster *F 56-126 Candida krusei 'Johnson Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 60-361 Candida krusei 'Peach orchard near Winters, 08/60' Isolated from Drosophila melanogaster *F 61-114 Candida magnoliae 'Berryessa Hills near Winters, CA 2/14/61' isolated from Drosophila pseudoobscura (male) *F 76-9 Candida new species isolated from Drosophila carbonaria breeding in mesquite flux by W. Starmer (Starmer#75-156.4) *F 76-10 Candida new species isolated from Drosophila carbonaria breeding in mesquite flux by W. Starmer (Starmer#75-159.4) *F 73-507.2 Candida new species Hawaii Isolated from rotting cheirodendron branch with drosophila larvae at 3900 ft elevation. Island of Hawaii. *F 73-680.1 Candida new species Island of Hawaii. 'Isolated from full clear crop of Drosophila sordidapex, male.' *F 73-686 Candida new species Island of Hawaii. 'Isolated from full crop of Drosophila setosifrons, male.' *F 73-506.2 Candida new species hawaii Isolated from rotting cheirodendron branch with drosophila larvae at 3900 ft elevation. Island of Hawaii. *F 61-254 Candida new species 'Cedar Pass, N.E. California, August 1961' Isolated from Drosophila pseudoobscura *F 61-260 Candida new species 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila pseudoobscura *F 61-266 Candida new species 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila pseudoobscura *F 60-376 Candida new species 'Peach Orchard near Winters, 11/60' Isolated from Drosophila pseudoobscura *F 61-130 Candida new species 'Peach orchards, near Winters, CA 2/14/61' isolated from Drosophila pseudoobscura *F 82-543.1 Candida new species 'Site YaYa, Dominican Republic, Caribbean' Selenocereus flower with Drosophila larvae in flower *F 51-150 Candida new species 'Aspen Valley, Yosemite area, California 07/51' Isolated from Drosophila occidentalis *F 52-160 Candida nitratophila by D1D2 'Mather, Yosemite area, Calif. 06/52' California Isolated from ex Drosophila pseudoobscura *F 55-412 Candida parapsilosis Drosophila *F 51-223 Candida parapsilosis Central Sierra Nevada Mnts. Isolated from Drosophila persimilis *F 51-151 Candida parapsilosis 'Aspen Valley, Yosemite area, Calif., 07/51' California Isolated from Drosophila occidentalis *F 52-159 Candida parapsilosis 'Mather, Yosemite area, Calif. 06/52' California Isolated from ex Drosophila pseudoobscura *F 61-155 Candida parapsilosis 'Peach orchard, near Davis.' California Drosophila pseudoobscura *F 51-217 Candida parapsilosis 'Porcupine Flat, Yosemite National Park' California Isolated from Drosophila miranda *F 54-200 Candida parapsilosis 'Smoky Jack, Yosemite National Park' California Isolated from Drosophila occidentalis *F 54-201 Candida parapsilosis 'Smoky Jack, Yosemite National Park' California Isolated from Drosophila occidentalis *F 54-202 Candida parapsilosis 'Smoky Jack, Yosemite National Park' California Isolated from Drosophila occidentalis *F 54-204 Candida parapsilosis 'Smoky Jack, Yosemite Natl. Park' California Isolated from Drosophila occidentalis *F 54-212 Candida parapsilosis 'Smoky Jack, Yosemite Natl. Park' California Isolated from Drosophila occidentalis *F 57-100 Candida parapsilosis 'Wild Rose Canyon, Southern Sierra Nevada' California Isolated from Drosophila Pseudoobscura *F 62-1037 Candida parapsilosis 'Class isolate in FS&T 216, 5/62 Male Drosophila fly.' *F 53-55 Candida parapsilosis J.A. Recca, student of Marty Miller 'Isolated from a fruit fly (Drosophila) in a citrus processing plant, 1950' *F 50-2 Candida parapsilosis Shehata 'Berkeley, California' Isolated from Drosophila sp. at a vinegar factory *F 50-20 Candida parapsilosis Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 57-96 Candida rugosa 'Wild Rose Canyon, Panamint Mnts, southern Sierras' California Drosophila pseudoobscura *F 50-207 Candida rugosa Shehata 'Mountain Center, San Jacinto Mnts., So. Calif.' California Isolated from Drosophila pseudoobscura *F 73-127.2 Candida sake Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-129 Candida sake Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-140 Candida sake Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-141 Candida sake Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-123 Candida sake Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders).' *F 76-809.1 Candida sake on the island of Hawaii isolated from Drosophila or Sapinda *F 76-793.1 Candida sake on the island of Hawaii isolated from Sapinda or Drosophila *F 76-800.1 Candida sake on the island of Hawaii isolated from Sapinda or Drosophila *F 76-839.3 Candida sake on the island of Hawaii isolated from Sapinda or Drosophila *F 76-846.1 Candida sake on the island of Hawaii isolated from Sapinda or Drosophila *F 72-332 Candida sake 'At Patrick Point State Park, Trinidad, Calif.' California Isolated from mushroom fruiting bodies with Drosophila larvae & eggs *F 72-333 Candida sake 'At Patrick Point State Park, Trinidad, Calif.' California Isolated from mushroom fruiting bodies with Drosophila larvae & eggs. *F 72-335 Candida sake 'At Patrick Point State Park, Trinidad, Calif.' California Isolated from mushroom fruiting bodies with Drosophila larvae & eggs. *F 72-337 Candida sake 'At Patrick Point State Park, Trinidad, Calif.' California Isolated from mushroom fruiting bodies with Drosophila larvae & eggs. *F 72-330 Candida sake 'At Patrick Point State Park, Trinidad, Calif.' California Isolated from mushroom fruiting bodies with Drosophila larvae and eggs & 1st instar Drosophila *F 61-153 Candida sake 'Peach orchard, near Winters, CA 5/4/61' isolated from Drosophila pseudoobscura (female) *F 61-246 Candida sake? 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila occidentalis *F 73-127.1 Candida santamariae Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-128 Candida santamariae Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-139 Candida santamariae Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-120 Candida silvae by D1D2 Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders).' *F 73-132 Candida silvatica Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 75-57 Candida sorbosa Hawaii From feces of the Hawaiian Drosophila crucigera by Hedrick & Burk. *F 73-634.2 Candida sp. Island of Hawaii. Isolated from lower surface of Cheirodendron leaf; Drosophila egg present. *F 73-674.1 Candida stellata Island of Hawaii. Isolated from Drosophila engiocracea. *F 73-674.3 Candida stellata Island of Hawaii. Isolated from Drosophila engiocracea. *F 61-521 Candida stellata 'Beryessa Hills, Calif. September 1961' California Isolated from Drosophila melanogaster *F 61-536 Candida stellata 'Fruit orchard near Winters, Oct. 1961' Isolated from Drosophila pseudoobscura *F 51-259 Candida stellata 'Mather, Yosemite area of California, 09/51' Isolated from Drosophila pinicola, Sierra Nevada Mts. *F 51-142 Candida vini 'Mather, Sierra Nevada, 07/51' California Isolated from Drosophila azteca *F 61-181 Citeromyces matritensis 'Berryessa Hills, near Winters, CA 6/20/61' Drosophila pseudoobscura *F 61-256 Citeromyces matritensis 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila occidentalis *F 61-251 Citeromyces matritensis 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila pseudoobscura *F 85-885.1 Clavispora opuntiae Ironwood Park, Tucson Mnts, AZ Drosophila nigrospiracula on rotting saguara *F 73-135 Cryptococcus aerius Northwest California 'From mushrom top with Drosophila eggs and larvae Near Orleans (Trinidad) N.W. Calif. W. Heed, 6/19/73.' *F 61-121 Cryptococcus kuetzingii 'Peach orchards near Winters, CA 2/14/61' isolated from Drosophila pseudoobscura (male) *F 60-413 Cryptococcus laurentii 'Peach orchard near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 52-274 Cryptococcus laurentii Phaff, Miller & Shifrine 'Porcupine Flat, Yosemite area, Calif. 06/54' California Isolated ex Drosophila pinicola *F 60-422 Cryptococcus macerans 'Peach orchard near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 55-475 Cryptococcus sp. 'Isolated from Drosophila melanogaster, 1955' *F 73-126 Cystofilobasidium infirmominiatum Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 73-515.3 Cystofilobasidium infirmominiatum at Bird Park, Kipuka puaulu, Island of Hawaii.' 'Isolated from Drosophila mimica *F 72-331 Cystofilobasidium infirmominiatum 'At Patrick Point State Park, Trinidad, Calif.' California Isolated from mushroom fruiting bodies with Drosophila larvae & eggs. *F 72-356 Cystofilobasidium infirmominiatum 'At Prairy Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae present. *F 61-182 Debaryomyces carsonii 'Berryessa Hills, near Winters, CA 6/20/61' Drosophila pseudoobscura *F 76-801.1 Debaryomyces hansenii on the island of Hawaii isolated from Drosophila - Cheirodendron *F 61-164 Debaryomyces hansenii 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 61-129 Debaryomyces hansenii 'Peach orchards, near Winters, CA 2/14/61' isolated from Drosophila pseudoobscura *F 61-101 Debaryomyces hansenii var. hansenii 'Berryessa Hills, near Winters, CA 2/3/61' isolated from Drosophila pseudoobscura (fem.) *F 60-379 Debaryomyces hansenii var. hansenii 'Peach orchard near Winters, 11/60' Isolated from Drosophila pseudoobscura *F 75-80 Debaryomyces polymorphus Shehata Berkeley Isolated from Drosophila sp. in a vinegar factory *F 76-14 Debaryomyces prosopidis isolated from Drosophila carbonaria near flux of mesquite tree by W.T. Starmer (Starmer#75-148.2) *F 76-15 Debaryomyces prosopidis isolated from Drosophila carbonaria near flux of mesquite tree by W.T. Starmer (Starmer#75-150.5) *F 76-16 Debaryomyces prosopidis isolated from Drosophila carbonaria near flux of mesquite tree by W.T. Starmer (Starmer#75-155.5) *F 84-102 Debaryomyces prosopidis Southern Arizona Isolated from Drosophila carbonaria breeding in mesquite flux (Prosopis juliflora) *F 50-6 Dipodascopsis uninucleata Berkeley Isolated from Drosophila sp. in a vinegar factory *F 51-266 Galactomyces geotrichum 'Mather, Yosemite area, California, 09/51' Isolated from Drosophila pinicola *F 50-64 Galactomyces geotrichum 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 61-296 Geotrichum fermentans 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila melanogaster *F 52-260 Geotrichum sp. Phaff, Miller & Shifrine 'Mather, Yosemite area, Calif. 07/52' California Isolated ex Drosophila persimilis *F 60-405 Hanseniaspora osmophila 'Hills near Winters, Calif. ; Dec. 1960' California Ex crop of Drosophila pseudoobscura *F 55-317 Hanseniaspora uvarum 'Isolated from Drosophila melanogaster, 1955.' *F 76-811.1 Hanseniaspora uvarum on the island of Hawaii isolated from Drosophila - Cheirodendron *F 56-121 Hanseniaspora uvarum 'Alta Sierra Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 56-122 Hanseniaspora uvarum 'Alta Sierra Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies. *F 56-117 Hanseniaspora uvarum 'Andrews Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 61-277 Hanseniaspora uvarum 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila Pseudoobscura *F 61-297 Hanseniaspora uvarum 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila melanogaster *F 61-502 Hanseniaspora uvarum 'Fruit orchard near Winters, September, 1961' Isolated from Drosophila melanogaster *F 56-124 Hanseniaspora uvarum 'Johnson Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 56-123 Hanseniaspora uvarum 'Johnson Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies. *F 60-375 Hanseniaspora uvarum 'Peach orchard near Winters, 11/60' Isolated from Drosophila pseudoobscura *F 60-383 Hanseniaspora uvarum 'Peach orchard near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 61-151 Hanseniaspora uvarum 'Peach orchard, near Winters, CA 5/4/61' isolated from Drosophila pseudoobscura *F 61-160 Hanseniaspora uvarum 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 61-169 Hanseniaspora uvarum 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 61-516 Hanseniaspora valbyensis 'Beryessa Hills, California, Sept. 1961' Isolated from Drosophila melanogaster *F 56-113 Hanseniaspora valbyensis 'Fresno, CA. 08/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 56-110 Hanseniaspora valbyensis 'Fresno, CA. 08/56'California 'Isolated from a Calimyrna fig with emerging Drosophila flies, eggs and larvae' *F 60-360 Hanseniaspora valbyensis 'Peach orchard near Winters., 08/60' Isolated from Drosophila malanogaster *F 61-165 Hanseniaspora valbyensis 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 61-136 Issatchenkia occidentalis 'Berryessa Hills, near Winters, CA 4/61' isolated from Drosophila pseudoobscura *F 52-171 Issatchenkia occidentalis 'Mather, Sierra Nevada (Yosemite area) 1952' California Drosophila pinicola *F 70-15 Issatchenkia occidentalis 'Obtained from Delft, ex Drosophila salivary glands and reported to be pathogenic to Drosophila. Original # 2929B.' Ex Drosophila clones (salivary glands). *F 51-154 Issatchenkia orientalis 'Aspen Valley, Yosemite area, California 07/51' Isolated from Drosophila occidentalis *F 61-161 Issatchenkia orientalis 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 61-186 Issatchenkia orientalis 'Peach orchard, near Winters, CA 7/5/61' Isolated from Drosophila melanogaster *F 56-108 Issatchenkia terricola 'Fresno, CA.' California Isolated from Calimyrna fig with emerging Drosophila flies *F 56-107 Issatchenkia terricola 'Fresno, CA.' California Isolated from Calimyrna fig with emerging Drosophila flies. *F 56-111 Issatchenkia terricola 'Fresno, CA.' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 61-147 Kloeckera apiculata 'Berryessa Hills, near Winters, CA 5/4/61' isolated from Drosophila pseudoobscura *F 61-282 Kloeckera apiculata 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila Pseudoobscura *F 61-549 Kloeckera apiculata 'Fruit orchard near Winters, Nov. 1961' Isolated from Drosophila melanogaster *F 50-45 Kluyveromyces dobzhanskii 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 51-200 Kluyveromyces dobzhanskii 'Porcupine Flat, Yosemite Natl. Park , 08/51' California Isolated from Drosophila Òobscura groupÓ *F 60-407 Kluyveromyces lactis var. drosophilarum 'Beryessa Hills near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 51-130 Kluyveromyces lactis var. drosophilarum 'Mather, Central Sierra Nevada Mountains, CA, 07/51' California Isolated from Drosophila azteca *F 51-272 Kluyveromyces lactis var. drosophilarum 'Mather, Yosemite area, California, 09/51' Isolated from Drosophila pinicola *F 51-144 Kluyveromyces lactis var. drosophilarum 'Mather, Sierra Nevada, 07/51' California Isolated from Drosophila azteca *F 51-237 Kluyveromyces lactis var. drosophilarum 'Mather, Yosemite area, California, 09/51' Isolated from Drosophila pinicola *F 61-200 Kluyveromyces lactis var. drosophilarum 'Pacific Coast, Gualala, Northern CA 6/28/61' Isolated from Drosophila pseudoobscura *F 61-177 Kluyveromyces lactis var. drosophilarum 'Peach orchard, near Winters, CA 6/20/61' Drosophila pseudoobscura *F 50-80 Kluyveromyces lactis var. drosophilarum 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 50-81 Kluyveromyces lactis var. drosophilarum 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 52-163 Kluyveromyces lactis var. drosophilarum Phaff, Miller & Shifrine 'Mather, Yosemite area, Calif. 06/52' California Isolated ex Drosophila miranda *F 50-112 Kluyveromyces lactis var. drosophilarum Shehata 'Keen Camp, Southern Sierra Nevada Mnts.' California Isolated from Drosophila pseudoobscura *F 61-293 Kluyveromyces marxianus 'Winters fruit orchard, 09/61' Isolated from Drosophila melanogaster *F 50-178 Kluyveromyces marxianus Shehata 'Mountain Center on Mount San Jacinto, So. Calif.' California Isolated from Drosophila pseudoobscura *F 50-84 Kluyveromyces marxianus Shehata 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 50-16 Kluyveromyces marxianus Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 50-18 Kluyveromyces marxianus Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 50-29 Kluyveromyces marxianus Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 61-189 Kluyveromyces marxianus Sacto valley peach orchard isolated by Knapp from Drosophila melanogaster *F 61-104 Kluyveromyces thermotolerans Lake Barryessa area isolated from the crop of Drosophila pseudoobscura61-108 Candida magnoliae 'Peach orchard near Winters, CA 2/3/61' isolated from Drosophila pseudoobscura (male) *F 61-134 Kluyveromyces thermotolerans Lake Berryessa Area isolated from the crop of Drosophila pseudoobscura *F 61-140 Kluyveromyces thermotolerans Lake Berryessa Area isolated from the crop of Drosophila pseudoobscura *F 61-143 Kluyveromyces thermotolerans Lake Berryessa Area isolated from the crop of Drosophila pseudoobscura *F 61-518 Kluyveromyces thermotolerans Lake Beryessa area Isolated from crop of Drosophila melanogaster *F 61-519 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila melanogaster *F 60-373 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila pseudoobscura *F 60-386 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila pseudoobscura *F 60-395 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila pseudoobscura *F 60-403 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila pseudoobscura *F 60-406 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila pseudoobscura *F 61-510 Kluyveromyces thermotolerans Lake Beryessa area Isolated from the crop of Drosophila pseudoobscura *F 61-529 Kluyveromyces thermotolerans Valley fruit orchard Isolated from the crop of Drosophila melanogaster *F 61-537 Kluyveromyces thermotolerans Valley fruit orchard Isolated from the crop of Drosophila pseudoobscura *F 51-160 Kluyveromyces thermotolerans 'Aspen Valley, Yosemite area, Calif.' California Isolated from crop of Drosophila azteca *F 61-262 Kluyveromyces thermotolerans 'Cedar Pass, N.E. California' Isolated from the crop of Drosophila pseudoobscura *F 50-101 Kluyveromyces thermotolerans 'Keen Camp, Southern Sierra Nevada Mnts.' California Isolated from crop of Drosophila pseudoobscura *F 50-106 Kluyveromyces thermotolerans 'Keen Camp, Southern Sierra Nevada Mnts.' California Isolated from crop of Drosophila pseudoobscura *F 50-89 Kluyveromyces thermotolerans 'Keen Camp, So. Calif. Mtns.' California Isolated from crop of Drosophila pseudoobscura *F 51-171 Kluyveromyces thermotolerans 'Mather, Yosemite area, California' Isolated from crop of Drosophila pseudoobscura *F 52-222 Kluyveromyces thermotolerans 'Mather, Yosemite area, Calif. ' California From crop of Drosophila azteca *F 50-174 Kluyveromyces thermotolerans Shehata 'Jacksonville, Central Sierra Nevada Mtns.' California Isolated from Drosophila pseudoobscura *F 50-166 Kluyveromyces thermotolerans Shehata 'Jacksonville, Central Sierra Nevada Mtns.' California Isolated from crop of Drosophila pseudoobscura *F 50-208 Kluyveromyces thermotolerans Shehata 'Mountain Center, San Jacinto Mnts., So. Calif.' California Isolated from Drosophila pseudoobscura *F 50-15 Kluyveromyces thermotolerans Shehata 'Pinon Flats, San Jacinto Mnts. Southern California' Isolated from Drosophila pseudoobscura *F 54-213 Kluyveromyces wickerhamii Yosemite Creek 07/54 California Isolated from Drosophila montana *F 54-210 Kluyveromyces wickerhamii 'Aspen Valley, Yosemite area' California Isolated from Drosophila pinicola *F 61-294 Kodamaea ohmeri by D1D2 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila melanogaster *F 73-136 Leucosporidium scottii Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders) by W.B. Heed.' *F 72-359 Leucosporidium scottii 'At Prairie Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae. *F 60-397 Metschnikowia pulcherrima 'Beryessa Hills near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 61-275 Metschnikowia pulcherrima 'Fruit orchard near Winters, California, Sept., 1961' Isolated from Drosophila pseudoobscura *F 50-31 Metschnikowia pulcherrima 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 50-11 Metschnikowia pulcherrima Shehata 'Berkeley, Calif.' California Isolated from Drosophila sp. in a vinegar factory *F 50-26 Metschnikowia pulcherrima Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 50-28 Metschnikowia pulcherrima Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 85-886.3 Phaffomyces thermotolerans Ironwood Park near Tucson, Arizona 'Isolated from Drosophila mettleri' *F 85-892.1 Phaffomyces thermotolerans Ironwood Park near Tucson, Arizona 'Isolated from Drosophila nigrospiracula' *F 85-890.3 Phaffomyces thermotolerans Ironwood Park near Tucson, Arizona' 'Isolated from Drosophila nigrospiracula *F 85-888.2 Phaffomyces thermotolerans Ironwood Park, Tucson Mnts., AZ 'Isolated from Drosophila nigrospiracula Ironwood Park near Tucson, Arizona' *F 80-204 Phaffomyces thermotolerans 'El Arenoso, Baja Calif. Norte, Mexico' Cactus specific. Drosophila larvae on Pachycereus pringlei (cardon) rot *F 60-394 Pichia angusta 'Beryessa Hills near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 51-138 Pichia angusta 'Mather, Sierra Nevada, 07/51' California Isolated from Drosophila pseudoobscura 51-140 Hanseniaspora osmophila 'Mather, Sierra Nevada, 07/51' California Isolated from Drosophila pseudoobscura *F 61-235 Pichia angusta 'Pacific coast near Gualala River, June, 1961' Isolated from Drosophila viridis *F 52-251 Pichia angusta 'Mather, Sierra Nevada, Calif.' California Isolated from Drosophila pseudoobscura *F 61-541 Pichia anomala 'Beryessa Hills, 10/17/61' Isolated from Drosophila melanogaster *F 72-101 Pichia cactophila San Agusta, Sonora, Mexico 'Isolated by W.B. Heed from larval gut of Drosophila pachea Orig. A 309 gut 2.' *F 77-494.5 Pichia cactophila Tucson, AZ Crop content of Drosophila mettleri *F 71-156 Pichia cactophila Tucson, Arizona. 'Isolated from larval gut of Drosophila rot saguaro at Tucson, Arizona.' *F 77-355.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-359.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-378.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-379.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-387.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-391.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-393.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 77-404.1 Pichia cactophila 'El Secorro, Baja California, Norte Mexico' Drosophila mojavensis *F 50-44 Pichia cactophila 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 77-464 Pichia cactophila 'Tucson, Arizona' Drosophila metleri male *F 77-472 Pichia cactophila 'Tucson, Arizona' Drosophila nigrospiracula male *F 72-323 Pichia cactophila 'at Silver Bell site, Tucson, Arizona 9/72.' Isolated from wet soil under saguaro with larvae of Drosophila mettleri. *F 72-324 Pichia cactophila 'at Silver Bell site, Tucson, Arizona 9/72.' Isolated from wet soil under saguaro with larvae of Drosophila mettleri. *F 76-213 Pichia cactophila Phaff La Paz, Baja California Sur, Mexico, near airport Agria cactus, very wet fermenting, Drosophila mojavensis adults seen *F 61-158 Pichia fermentans 'Peach orchard, near Winters, CA 5/4/61' Drosophila pseudoobscura *F 56-119 Pichia guilliermondii 'Alta Sierra Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 61-162 Pichia guilliermondii 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 50-19 Pichia guilliermondii Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated form Drosophila pseudoobscura *F 56-106 Pichia guilliermondii 'Alta Sierra Ranch, Fresno, CA. 08/56' California 'Isolated from Calimyrna fig, containing Drosophila eggs and larvae + adults.' *F 77-493.2 Pichia heedii Crop content of Drosophila mettleri *F 77-470.2 Pichia heedii near Tucson, Arizona 'Isolated from Drosophila nigrospiracula .' *F 77-480 Pichia heedii near Tucson, Arizona 'Isolated from Drosophila nigrospiracula .' *F 77-493.1 Pichia heedii (imperfect) Tucson, AZ Crop content of Drosophila mettleri *F 77-465.2 Pichia heedii imperfect Tucson AZ Crop of Drosophila nigrospiracula *F 60-411 Pichia holstii 'Beryessa Hills near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 85-891.3 Pichia kluyveri var. kluyveri Ironwood Park, Tucson Az Drosophila nigrospiracula *F 73-121 Pichia kluyveri var. kluyveri Pacific Northwest 'Isolated from Drosophila breeding sites P.N.W. (mushrooms, baytrees, alders).' *F 61-520 Pichia kluyveri var. kluyveri 'Beryessa Hills, Calif. Sept., 1961' California Isolated from Drosophila melanogaster *F 56-115 Pichia kluyveri var. kluyveri 'Fresno, CA. 08/56' California Isolated from a Calimyrna fig with emerging Drosophila melanogaster *F 61-525 Pichia kluyveri var. kluyveri 'Fruit orchard near Winters, Oct. 1961' Isolated from Drosophila melanogaster *F 61-503 Pichia kluyveri var. kluyveri 'Fruit orchard near Winters, Sept. 1961' Isolated from Drosophila melanogaster *F 56-125 Pichia kluyveri var. kluyveri 'Johnson Ranch, Fresno, CA. 09/56' California Isolated from a Calimyrna fig with emerging Drosophila flies *F 84-663.4 Pichia kluyveri var. kluyveri 'Ohara, Austrailia' Isolated from female Drosophila buzzatii by Starmer *F 84-660.3 Pichia kluyveri var. kluyveri 'OÕhara, Austrailia' Isolated from Drosophila buzzatii by Starmer. *F 60-377 Pichia kluyveri var. kluyveri 'Peach orchard near Winters, 11/60' Isolated from Drosophila pseudoobscura *F 61-171 Pichia kluyveri var. kluyveri 'Peach orchard, near Winters, CA 6/12/61' Drosophila pseudoobscura *F 70-14 Pichia membranifaciens 'Ex Drosophila clones (salivary glands), Delft, reported to be pathogenic to Drosophila. Original # 2929A.' *F 76-811.2 Pichia membranifaciens on the island of Hawaii isolated from Drosophila - Cheirodendron *F 61-265 Pichia membranifaciens 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila pseudoobscura *F 61-187 Pichia membranifaciens 'Peach orchard, near Winters, CA 7/5/61' Isolated from Drosophila melanogaster *F 61-132 Pichia membranifaciens Phaff 'Peach orchards, near Winters, CA 2/14/61' isolated from Drosophila pseudoobscura *F 84-797.3 Pichia nakasei W.T.Starmer 'Trinkey, N.S.W. Australia.' Isolated from Drosophila buzzatii. *F 50-24 Pichia norvegensis Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 52-155 Pichia pastoris 'Mather, Yosemite area, Calif. 06/52' California Isolated from Drosophila pseudoobscura *F 50-165 Pichia pastoris Shehata 'Jacksonville, Central Sierra Nevada Mtns.' California Isolated from Drosophila pseudoobscura *F 50-97 Pichia pastoris Shehata 'Keen Camp, Southern Sierra Nevada Mnts.' California Isolated from Drosophila pseudoobscura *F 60-372 Pichia pijperi 'Peach orchard near Winters, 11/60' Isolated from Drosophila melanogaster *F 83-501.2 Pichia pseudocactophila Crop of surface -sterilized Drosophila nigrospiracula on fallen rotting saguara *F 83-514.2 Pichia pseudocactophila Ironwood Park, Tucson Mnts, AZ Fly obtained from fallen rotting saguaro. Live Drosophila nigrospiracula on agar plate *F 83-518.2 Pichia pseudocactophila Ironwood Park, on fallen rotting saguaro Live Drosophila nigrospiracula on plate *F 77-466.3 Pichia pseudocactophila by D1D2 Crop of Drosophila mettleri, female, Tucson, AZ *F 77-465.1 Pichia pseudocactophila by D1D2 Tucson AZ Crop of Drosophila nigrospiracula *F 83-505.2 Pichia pseudocactophila? Ironwood Park, fallen rotting saguaro,Tucson Mnts,AZ Drosophila nigrospiracula crop (surface sterilized fly) *F 83-506.1 Pichia pseudocactophila? Ironwood Park,Tuscon Mnts, on fallen rotting saguaro Surface-sterilized Drosophila nigrospiracula crop content *F 61-213 Pichia sp. 'Gualala River, Pacific Coast, northern Calif.' California Drosophila pseudoobscura *F 52-167 Pichia sp. Phaff, Miller & Shifrine 'Mather, Yosemite area, California. 06/52' Isolated ex Drosophila occidentalis *F 57-103 Pichia subpelliculosa 'Wild Rose Canyon, Southern CA Mnts.' Isolated from Drosophila pseudoobscura *F 52-162 Pichia xylosa Phaff, Miller & Shifrine 'Mather, Yosemite area, Calif. 06/52' California Isolated from ex Drosophila miranda *F 51-197 Pichia xylosa by D1D2 'Porcupine Flat, Yosemite National Park' California Isolated from Drosophila persimilis *F 61-102 Rhodotorula aurantiaca 'Berryessa Hills, near Winters, CA 2/3/61' isolated from Drosophila pseudoobscura (fem.) *F 73-680.2 Rhodotorula fujisanensis Island of Hawaii Full clear crop of drosophila sordidapex *F 73-562.1 Rhodotorula fujisanensis Island of Hawaii, kipuka 4140 (N57) Brown Cheirodendron leaf with Drosophila eggs. *F 73-562.2 Rhodotorula fujisanensis Island of Hawaii, kipuka 4140 (N57) Brown Cheirodendron leaf with Drosophila eggs *F 72-363 Rhodotorula fujisanensis 'At Prairie Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves No Drosophila larvae present. *F 72-360 Rhodotorula fujisanensis 'At Prairie Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae. *F 72-361 Rhodotorula minuta 'At Prairie Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae. *F 54-190 Rhodotorula mucilaginosa Hedrick & Burke 'Oahu, Hawii' Isolated from Drosophila larvae *F 72-353 Rhodotorula muscorum 'At Prairy Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae present. *F 72-355 Rhodotorula muscorum by D1/D2 'At Prairy Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae present. *F 72-354 Rhodotorula new species 'At Prairy Creek State Park, Trinidad, Calif. by W.B. Heed..' California Isolated from fermenting bay tree leaves with Drosophila larvae present. *F 61-137 Saccharomyces bayanus 'Berryessa Hills, near Winters, CA 4/7/61' isolated from Drosophila pseudoobscura *F 51-206 Saccharomyces bayanus 'Porcupine Flat, Yosemite Natl. Park 08/51' California Isolated from Drosophila Òobscura groupÓ *F 61-196 Saccharomyces cerevisiae 'Pacific Coast, Gualala, Northern CA 6/28/61' Isolated from Drosophila pseudoobscura *F 61-232 Saccharomyces cerevisiae 'Pacific coast near Gualala River, June, 1961' Isolated from Drosophila viridis *F 61-190 Saccharomyces cerevisiae 'Peach orchard, near Winters, CA 7/28/61' Isolated from Drosophila pseudoobscura *F 79-42 Saccharomyces cerevisiae 'Sierra Nevada, California, obtained from CBS under #2709' isolated from Drosophila pinicola by H.J. Phaff *F 72-164 Saccharomyces cerevisiae Phaff Kipuka Ki in Hawaiian Volcanoes Natl. Park, Hawaii. Isolated 3/72 from wet brown flux of Acacia koa with Drosophila hawaiiensis and honey bees. *F 61-269 Saccharomyces kluyveri 'Cedar Pass, N.E. California, August, 1961' Isolated from Drosophila pseudoobscura *F 61-204 Saccharomyces kluyveri 'Gualala River, Pacific Coast, Northern Calif.' California Ex Drosophila pseudoobscura *F 51-242 Saccharomyces kluyveri 'Mather, Yosemite area of California, 09/51' Isolated from Drosophila pinicola *F 61-234 Saccharomyces kluyveri 'Pacific coast near Gualala River, June, 1961' Isolated from Drosophila pseudoobscura *F 57-78 Saccharomyces kluyveri 'Wild Rose Canyon, Panamint Mnts' Isolated from Drosophila pseudoobscura by Snyder and Phaff *F 51-137 Saccharomyces paradoxus 'Mather, Central Sierra Nevada, 07/51' California Isolated from Drosophila azteca *F 51-240 Saccharomyces paradoxus 'Mather, Yosemite area, California 09/51' Isolated from Drosophila californica *F 51-186 Saccharomyces paradoxus 'Mather, Yosemite area, California 08/51' Isolated from Drosophila pseudoobscura *F 52-153 Saccharomyces paradoxus M. Shifrine 'Yosemite area, Calif. 06/52' California Isolated from Drosophila (Obscura group) *F 51-110 Saccharomyces sp. Yosemite region of Calif. California 'Isolated from Drosophila fly , 1969' *F 68-378 Sporidiobolus salmonicolor S. Calif. California 'From intestinal tract of Drosophila by Recca and Mrak, 1952, in citrus processing plant, S. Calif.' *F 85-891.2 Sporopachydermia cereana-like Ironwood Park near Tucson, Arizona 'Isolated from Drosophila nigrospiracula' *F 77-399.1 Starmera amethionina var. amethionina 'El Sorocco, Baja Calif. Norte, Mexico' Isolated from Drosophila mojavensis *F 85-884.3 Starmera amethionina var. pachycereana Ironwood Park, Tucson Mnts, AZ 'Isolated from Drosophila nigrospiracula, near rotting saguaro,' *F 85-894.1 Starmera amethionina var. pachycereana Tucson Mountains, Arizona. 'Isolated from Drosophila nigrospiracula,' *F 76-216 Starmera amethionina var. pachycereana 'Rancho Cunano, Baja California Sur, Mexico' isolated from Lophocereus schottii (senita) rot attracted Drosophila pachea *F 62-1017 Torulaspora delbrueckii 'Class isolate from FS&T 216, 5/62. Source: Drosophila fly' *F 61-539 Torulaspora delbrueckii Sact. Valley fruit orchard 10/17/61 Isolated from Drosophila pseudoobscura *F 61-138 Torulaspora delbrueckii 'Berryessa Hills, near Winters, CA 4/7/61' isolated from Drosophila pseudoobscura *F 61-184 Torulaspora delbrueckii 'Berryessa Hills, near Winters, CA 6/20/61' isolated from Drosophila pseudoobscura *F 60-391 Torulaspora delbrueckii 'Beryessa Hills near Winters, 12/60' Isolated from Drosophila pseudoobscura *F 61-504 Torulaspora delbrueckii 'Orchard in Winters, 09/25/61' Isolated from Drosophila melanogaster *F 61-284 Torulaspora delbrueckii 'Orchard near Winters, California, 09-25-61' Isolated from Drosophila pseudoobscura *F 50-21 Torulaspora delbrueckii Shehata 'Pinon Flats, San jacinto Mnts., Southern California' Isolated from Drosophila pseudoobscura *F 82-543.2 Torulaspora delbrueckii like 'Site YaYa, Dominican Republic, Caribbean' Selenocereus flower with Drosophila larvae in flower *F 61-193 Williopsis saturnus var. saturnus peach orchard (7/28/61) Isolated from Drosophila pseudoobscura *F 50-46 Yarrowia lipolytica 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 50-47 Yarrowia lipolytica Shehata. 'Pinon Flats, San Jacinto Mnts., Southern Calif.' California Isolated from Drosophila pseudoobscura *F 50-140 Yarrowia lipolytica Shehata 'Berkeley, Calif.' California Isolated from Drosophila sp.at a vinegar factory *F 61-285 Zygosaccharomyces bailii 'Fruit Orchard near Winters, California, Sept., 1961' Isolated from Drosophila pseudoobscura *F 61-207 Zygosaccharomyces bailii 'Gualala River, pacific coast, northern Calif.' California Ex Drosophila pseudoobscura *F 61-192 Zygosaccharomyces fermentati ' Peach orchard, near Winters, CA 7/28/61' Isolated from Drosophila pseudoobscura *F 51-118 Zygosaccharomyces fermentati 'Mather, Central Sierra Nevada, 07/51' California Isolated from Drosophila azteca *F 51-188 Zygosaccharomyces fermentati 'Mather, Yosemite area, California 08/51' Isolated from Drosophila pseudoobscura *F 61-237 Zygosaccharomyces fermentati 'Pacific coast near Gualala River, June, 1961' Isolated from Drosophila pseudoobscura *F 51-212 Zygosaccharomyces fermentati 'Porcupine Flat, Yosemite National Park, 08/51' California Isolated from Drosophila Òobscura groupÓ *F 52-164 Zygosaccharomyces fermentati Phaff, Miller & Shifrine 'Mather, Yosemite area, Calif. 06/52' California Isolated ex Drosophila azteca *F 61-511 Zygosaccharomyces fermentati H. Naganishi 'Beryessa Hills, California, Sept. 1961' Isolated from Drosophila pseudoobscura *F 52-198 Zygosaccharomyces fermentati Phaff, Miller & Shifrine 'Mather, Yosemite area, Calif. 06/52' California Isolated ex Drosophila azteca *F 51-146 Zygosaccharomyces fermentati 'Mather, Sierra Nevada, 07/51' California Isolated from Drosophila azteca *F 51-198 Zygosaccharomyces fermentati 'Porcupine Flat, Yosemite Natl. Park 08/51' California Isolated from Drosophila persimilis *F 51-203 Zygosaccharomyces fermentati 'Porcupine Flat, Yosemite Natl. Park, 08/51' California Isolated from Drosophila occidentalis *F 51-208 Zygosaccharomyces florentinus 'Porcupine Flat, Yosemite Natl. Park 08/51' California Isolated from Drosophila persimilis *F 72-279 Zygosaccharomyces microellipsoides? Drosophila crop # *U FBrf0173059 *a Thor *b S. *t 2003.9.29 *T personal communication to FlyBase *u *F From stefan_thor@hms.harvard.edu Mon Sep 29 14:49:01 2003 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Mon, 29 Sep 2003 14:49:01 \+0100 *F Dear Chihiro, *F .. *F UAS-EGFPF *F =========== *F The membrane-targeted reporter UAS-EGFPF used in our study was *F generated (by us) by N-terminal insertion of 6 copies of the c-myc *F epitope tag into the previously generated UAS-EGFPF (Finley et al. *F 1998), a C-terminal insertion into EGFP of the carboxy-terminal 20 *F amino acids of c-Ha-Ras containing membrane-targeting farnesylation *F and palmitoylation sequences (Jiang and Hunter, 1998). *F Finley et al. construct, denoted UAS-EGFPF: UAS::EGFP::CAAX *F Allan et al. construct, denoted UAS-EGFPF: UAS::6xMyc::EGFP::CAAX *F Finley, et al. (1998). Neuron 21, 1363-1374. *F Jiang, W., and Hunter, T. (1998). Biotechniques 24, 348-354. *F We should have called it UAS-myc-EGFPF. This was a mistake. *F Hope that this is helpful, best wishes, *F \-- *F Stefan Thor Ph.D. *F Assistant Professor of Neurobiology *F Department of Neurobiology, GB 504 *F Harvard Medical School *F 220 Longwood Avenue *F Boston, MA 02115 *F Phone: (617) 432-2351(of) \-2352(lab) *F Fax: (617) 734-7557 *F sthor@HMS.Harvard.edu *F FEDEX acc.no: 1599-9330-2 *F http://neuro.med.harvard.edu/site/faculty/thor.html # *U FBrf0173062 *a Wharton *b K. *t 2003.10.8 *T personal communication to FlyBase *u *F From kristi_wharton@brown.edu Wed Oct 08 14:31:09 2003 *F Envelope-to: cy200@gen.cam.ac.uk *F Delivery-date: Wed, 8 Oct 2003 14:31:09 \+0100 *F Subject: Re: FlyBase Query *F Dear Chihiro, *F There seems to be some confusion in the source of the various UAS-gbb *F lines. It is true that the I received three UAS-gbb lines from Mike *F Hoffmann as noted below but these are NOT the lines that were used in *F the Khalsa et al 1998 paper. In that paper, we described making a *F construct and recovering four UAS-gbb transgenic lines. These lines *F are distinct from the three made in the Hoffmann lab. Our construct *F makes use of only gbb cDNA sequences, with the endogenous polyA *F signal. We made the new lines because we were unable to rescue gbb *F mutants with the fly stocks we received from Mike Hoffmann. It was *F never clear why Mike's lines did not completely rescue gbb mutants in *F our hands. I discussed the problem with Karen Staehling-Hampton at *F one point but we never resolved the problem. *F In the Khalsa etal 1998 paper, we unfortunately did not name the four *F new lines we generated because for the experiments described there we *F did not observe dramatic differences between the lines. However, *F since that paper, when we have sent the lines out we have been *F careful to denote the line number should it be important in future *F experiments. *F They were designated: *F UASgbb9.1 on chr III *F UASgbb9.9 on chr II *F UASgbb9.7 on chr X *F UASgbb9.8 on chr II *F I hope this helps to clarify the confusion. Of course, if you have *F any further questions please feel free to contact me. *F Regards, *F Kristi *F \-- *F Kristi A. Wharton *F Associate Professor *F Department of Molecular Biology, Cell Biology and Biochemistry *F Brown University *F Box G-J160 *F Providence, R.I. 02912 *F Phone: 401 863 1951 *F FAX: 401 863 2421 # *U FBrf0173063 *a Strutt *b D. *t 2003.10.8 *T personal communication to FlyBase *u *F Date: Wed, 8 Oct 2003 11:05:24 \+0100 (BST) *F From: David Strutt *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (cy2078) *F Dear Chihiro, *F .. *F Below, I've tried to compile a definitive list of the constructs actually *F made and used in the Mol Cell paper, and then I've listed where the *F Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs have been *F subsequently used, both in our publications and others. Hopefully this is *F a complete list, although I might have missed a usage by another group. *F .. *F Best wishes, *F David *F \* Constructs made and used in experiments described in Strutt, 2001, *F Molec. Cell 7(2): 367--375 *F Arm-Fz-EGFP *F Act5C-FRT-Stop-FRT-Fz-EYFP *F Act5C-FRT-Stop-FRT-FzP278L-EYFP *F Act5C-FzdIntra-EYFP *F UAS-Fz-EGFP *F UAS-FzP278L *F UAS-FzdIntra-EYFP *F Stable line with the FRT-Stop-FRT excised was generated for: *F Act5C-FRT-Stop-FRT-FzP278L-EYFP = Act5C-FzP278L-EYFP *F (Note that I didn't generate a stable line of the form Act5C-Fz-EYFP \- *F expression of Fz-EYFP was always induced by co-expression of FLP in a *F Act5C-FRT-Stop-FRT-Fz-EYFP background.) *F For simplicity, both EGFP and EYFP are referred to as GFP in the *F manuscript. In the wing, both the Act5C and Arm promoter constructs give *F the same results, so I also don't distinguish between them in the text. *F \* Fz-GFP constructs used in subsequent publications *F Strutt et al., 2002, Curr. Biol. 12(10): 813--824 *F In this paper I used both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP *F transgenes. Arm-Fz-GFP is referred to as Fz-GFP in text and figures, *F whereas Act-FRT-Stop-FRT-Fz-EYFP is referred to as Fz-YFP (and is only *F used for one mosaic experiment in figure 4). *F Strutt and Strutt, 2002, Dev. Cell 3(6): 851--863 *F Here again, both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes are *F used. In figure 1, where Fz-GFP distribution in dsh and ds clones in *F analysed, I used Arm-Fz-GFP and referred to it as Fz-GFP. In the *F experiments in figures 2 and 3, where we induce Fz-GFP expression using *F hsFLP1, we use the original Act5C-FRT-Stop-FRT-Fz-EYFP transgene from the *F Mol Cell paper (and often abbreviated it to >>Fz on the figure panels). *F Strutt and Strutt, 2003, Current Biology 13(16):1451--1457 *F Here we used only Arm-Fz-EGFP. *F \* Our Fz-GFP constructs used by other groups in subsequent publications *F Both Arm-Fz-EGFP and Act5C-FRT-Stop-FRT-Fz-EYFP transgenes have been *F distributed, depending upon what was requested and whether it seemed *F likely they would want to induce mosaic expression. However, I believe *F that all the published examples used Arm-Fz-EGFP. *F To my knowledge these are: *F Tree et al., 2002, Cell 109(3): 371--381 *F Ma et al., 2003, Nature 421(6922): 543--547 *F Yang et al., 2002, Cell 108(5): 675--688 *F He & Adler, 2002, BMC Dev Biol. 2002 May 3;2(1):7 *F Adler, 2002, Dev Cell. 2002 May;2(5):525-35 *F \- although Ma et al and Yang et al don't actually show any pictures of *F Fz-GFP ('data not shown') *F \----------------------------------------------------------------------------- *F Dr. David Strutt *F Centre for Developmental Genetics *F Department of Biomedical Science *F University of Sheffield *F Western Bank *F Sheffield *F S10 2TN *F England *F Tel.: \+44 114 22 22 372 *F Fax.: \+44 114 22 22 788 *F e-mail: d.strutt@sheffield.ac.uk # *U FBrf0173106 *a Wehrli *b M. *t 2003 *T personal communication to FlyBase *u *F From wehrlim@ohsu.edu Thu Nov 06 20:47:27 2003 *F Envelope-to: djs93@gen.cam.ac.uk *F Delivery-date: Thu, 6 Nov 2003 20:47:27 \+0000 *F > 1. I have been unable to trace the construct 'UAS-fz2-eCFP'. Could *F you *F > send details of its construction or point me towards a reference *F where *F > I could get these details? *F We made the construct by fusing the eCFP (Clontech) with its start *F methionine to the last amino acid of fz2 (a gift of Jeremy Nathans) *F while inserting an aspartate inbetween (the rationale was to provide *F with the aspartate a carboxy group to replace the now blocked C-terminal *F carboxy group). Therefore the protein sequence at the junction is *F (fz2)AASHV-D-MVSKGE(eCFP). *F The construct was assembled from restriction fragments and PCR *F fragments by transforming the linear fragments into yeast. The *F fragments were designed to have at least 20bp overlap in sequence and *F yeast efficiently uses these overlaps to recombine the fragments and *F assemble the construct. *F Erdeniz N, Mortensen UH, Rothstein R. Cloning-free PCR-based allele *F replacement methods. Genome Res. 1997 Dec;7(12):1174-83. *F The resulting fz2-eCFP construct was cloned into the UAS vector we *F previously used followed by the insertion of a white-flip-out cassette *F (Wehrli and Tomlinson, 1998). When the flip-out cassette is removed the *F UAS promoter can drive expression of the cDNA. This construct is based *F on those generated by Gary Struhl. The resulting construct would be *F UAS> GMR-white flipout cass.> fz2-eCFP \- tub 3'UTR \- Carnegie2NX *F > 2. What is the origin/nature of the myristoylatation tag used in the *F > construct 'UAS-Myr-Arrintra'? *F MGNKCCSKRQ (as in Struhl and Adachi, Cell, 93, 649-660) *F The complete protein sequence (including the 6xFLAG tags) is *F MGNKCCSKRQFMKSAEDYKDDDDKDYKDDDDKDYKDDDDKGSDYKDDDDKDYKDDDDKDYKDDDDKLQFCRTRIGKSRT *F EPKDDQATDPLSPSTLSKSQRVSKIASVADAVRMSTLNSRNSMNSYDRNHITGASSSTTNGSSMVAYPINPPPSPATRS *F RRPYRHYKIINQPPPPTPCSTDICDESDSNYTSKSNSNNSNGGATKHSSSSAAACLQYGYDSEPYPPPPTPRSHYHSDV *F RIVPESSCPPSPSSRSSTYFSPLPPPPSPVQSPSRGFT *F Please let me know if you need addtional information. *F Best wishes, *F Marcel # *U FBrf0173129 *a Perotti *b M.E. *t 2003.10.14 *T personal communication to FlyBase *u *F To: elisa.perotti@unimi.it *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Fri, 3 Oct 2003 18:30:26 \+0100 *F Dear Dr. Perotti, *F I am curating your paper for FlyBase: *F Perotti et al., 2001, Molec. Reprod. Dev. 60(2): 248--259 *F on the 'casanova' (csn) mutation. *F In this paper you say that the mutation was isolated from a set of male *F sterile lines that were originally described in: *F Hackstein, 1991, Europ. J. Cell Biol. 56(2): 151--169 *F there are a number of named male sterile mutations in that paper, with *F names such as 'ms(3)HB74', 'ms(3)HB267' etc. *F do you know what the 'HB' number of your csn mutant is so that I can *F record this information in the database and if we already have a record *F of the mutation I can make sure we do not end up with 2 gene records in *F the database for the same mutation. *F .. *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Tue, 14 Oct 2003 19:07:21 \+0200 *F From: Maria Elisa Perotti *F Subject: Re: FlyBase query *F To: Gillian Millburn (Genetics) *F Dear Dr. Millburn, *F I apologize for the delay in replying to your letter. *F 'Casanova' (csn) was originally ms (3)HB 156. *F .. *F Thank you. *F Best regards. *F Maria-Elisa Perotti *F >Dear Dr. Perotti, *F > *F >I am curating your paper for FlyBase: *F > *F >Perotti et al., 2001, Molec. Reprod. Dev. 60(2): 248--259 *F > *F >on the 'casanova' (csn) mutation. *F > *F >In this paper you say that the mutation was isolated from a set of male *F >sterile lines that were originally described in: *F > *F >Hackstein, 1991, Europ. J. Cell Biol. 56(2): 151--169 *F > *F >there are a number of named male sterile mutations in that paper, with *F >names such as 'ms(3)HB74', 'ms(3)HB267' etc. *F > *F >do you know what the 'HB' number of your csn mutant is so that I can *F >record this information in the database and if we already have a record *F >of the mutation I can make sure we do not end up with 2 gene records in *F >the database for the same mutation. *F > *F .. *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- # *U FBrf0173133 *a Rutkowski *b R. *c B. *d Warren *t 2003.10.6 *T personal communication to FlyBase *u FlyBase error report for CG18176 on Sun Oct 5 20:37:49 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Sun, 5 Oct 2003 20:37:49 \-0700 *F To: flybase-updates@morgan.harvard.edu *F Cc: bill.warren@jcu.edu.au *F Subject: FlyBase error report for CG18176 on Sun Oct 5 20:37:49 2003 *F Error report from Rachael Rutkowski & Bill Warren (bill.warren@jcu.edu.au) *F Gene or accession: CG18176 *F Release: 3 *F Missed gene *F Comments: We have isolated 5 homozygous lethal P element insertions into *F CG18176. *F Lethal period varies between alleles and includes both larvae and pupae. *F Mutant larvae become sluggish and appear developmentally retarded. Pupal *F lethality is associated with severe defects in abdominal formation. We have *F named this gene 'deflated' (abbrev. defl) to reflect the pupal phenotype. # *U FBrf0173137 *a Dahmann *b C. *t 2003.11.6 *T personal communication to FlyBase *u *F To: dahmann@mpi-cbg.de *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Mon, 22 Sep 2003 13:53:05 \+0100 *F Dear Dr. Dahmann, *F I am curating a paper for FlyBase: *F Rintelen et al., 2003, Development 130(15): 3479--3490 *F in which the authors use 2 engrailed-GAL4 lines from you: *F en-GAL454 and en-GAL433 ( = superscript) *F I wrote to the authors about these lines as I was not sure whether or *F not we already have them in FlyBase, and Knud Nairz replied that 'Those *F lines have been generated from the lacZ enhancer trap line by *F gene-conversion. Possibly they have not been put into FlyBase.' *F so I am still unsure whether or not we have a record of these lines in *F FlyBase, although I suspect that they are new. *F I was hoping you could tell me the details of how you made the two *F lines so that I can get this information into the database (I would *F record this information as a personal communication from you). *F Since they were made by gene conversion, I would be grateful if you *F could tell me: *F a. the starting lacZ enhancer trap line, with its identifier number, *F e.g. 24B, A101. *F b. what is the GAL4 vector that was used for the gene conversion \- was *F it the GawB element ? *F c. are the '54' and '33' lines just two different cases of gene *F conversion that were recovered, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Tue, 04 Nov 2003 13:03:45 \+0100 *F Subject: Re: FlyBase query *F From: Christian Dahmann *F To: 'Gillian Millburn (Genetics)' *F Dear Gillian, *F The starting lacZ enhancer trap line for en-GAL454 and en-GAL433 ( = *F superscript) was enryXho25 (Hama et al. (1990) Genes and Development *F 4:1079-1093)). *F The GAL4 vector that was used for the gene conversion is unknown. *F The lines '54' and '33' are two independent cases of gene conversion. *F Regards, *F Christian Dahmann *F On 22.09.2003 2:53, 'Gillian Millburn (Genetics)' *F wrote: *F > Dear Dr. Dahmann, *F > *F > I am curating a paper for FlyBase: *F > *F > Rintelen et al., 2003, Development 130(15): 3479--3490 *F > *F > in which the authors use 2 engrailed-GAL4 lines from you: *F > *F > en-GAL454 and en-GAL433 ( = superscript) *F > *F > I wrote to the authors about these lines as I was not sure whether or *F > not we already have them in FlyBase, and Knud Nairz replied that 'Those *F > lines have been generated from the lacZ enhancer trap line by *F > gene-conversion. Possibly they have not been put into FlyBase.' *F > *F > so I am still unsure whether or not we have a record of these lines in *F > FlyBase, although I suspect that they are new. *F > *F > I was hoping you could tell me the details of how you made the two *F > lines so that I can get this information into the database (I would *F > record this information as a personal communication from you). *F > *F > Since they were made by gene conversion, I would be grateful if you *F > could tell me: *F > *F > a. the starting lacZ enhancer trap line, with its identifier number, *F > e.g. 24B, A101. *F > *F > b. what is the GAL4 vector that was used for the gene conversion \- was *F > it the GawB element ? *F > *F > c. are the '54' and '33' lines just two different cases of gene *F > conversion that were recovered, *F > *F > thanks, *F > *F > Gillian *F > *F > \-------------------------------------------------------------- *F > Gillian Millburn. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: gm119@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > *F > FlyBase: http://fbserver.gen.cam.ac.uk/ *F > \-------------------------------------------------------------- *F > # *U FBrf0173164 *a Jaffe *b E.K. *t 2003.11.20 *T personal communication to FlyBase *u *F Date: Thu, 20 Nov 2003 09:58:16 \-0500 *F From: Eileen Jaffe *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: FlyBase query *F Dear Gillian Millburn, *F Please see below for the answers to your queries. *F Gillian Millburn (Genetics) wrote: *F >Dear Dr. Jaffe, *F > *F >I am curating your paper for FlyBase: *F > *F >Kundrat et al., 2003, J. Biol. Chem. 278(33): 3125-31330 *F > *F >about Drosophila porphobilinogen synthase (DmPBGS) and I have a *F >question. *F > *F >In the materials you state that you sequenced an EST from ResGen which *F >corresponds to DmPBGS. *F > *F >Could you tell me the identifier number of the individual EST that *F >corresponds to DmPBGS, e.g. GH12345, LD16475. Alternatively, do you *F >know which CG gene prediction DmPBGS corresponds to ? *F > *F On the ResGen invoice, the item number was 98002 and the clone id is *F listed as RE50220 (plate 502, row b, col 8, library RE, vector pFlc1, *F antibiotic amp). *F The gene corresponds to CG10335 gene product and was identified as PBGS *F on the basis of a blast search that I ran on 4/26/2000. The record for *F CG10335 does not identify the gene as encoding porphobilinogen synthase *F (a.k.a. 5-aminolevulinic acid dehydratase). Although we confirmed the *F sequence, we did not deposit it in the databases. *F > *F >This information will allow me to make sure that the information in *F >your paper gets attached to the correct CG gene record in FlyBase, *F >otherwise we may end up with duplicate records in FlyBase for what is *F >actually the same gene, *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- *F > *F > *F Please feel free to contact me if you need additional information. *F \-- *F \----------------------------------- *F Eileen K. Jaffe, Ph.D. *F Member, Institute for Cancer Research *F Fox Chase Cancer Center *F 7701 Burholme Ave *F Philadelphia, PA 19111, U.S.A. *F phone: 215 728-3695 *F fax: 215 728-2412 *F e-mail: EK_Jaffe@fccc.edu *F \----------------------------------- # *U FBrf0173177 *a Schaefer *b U. *t 2003.12.4 *T personal communication to FlyBase *u *F To: uschaef@gwdg.de *F Subject: FlyBase query: Gottingen P-element lines *F From: Gillian Millburn (Genetics) *F Date: Wed, 3 Dec 2003 17:59:45 \+0000 *F Dear Dr. Schaefer, *F I am a curator working for FlyBase. I am curating the supplementary *F information from your paper Peter et al., 2002, EMBO Rpts 3(1): 34--38 *F which contains the information about all the Gottingen X chromosome *F P{lacW} insertion lines to get the information into the database and I *F have a few questions to try and clarify what is going on in a few of *F the lines. *F I got the supplementary information as a table from: *F ftp://ftp.ebi.ac.uk/pub/databases/edgp/200111/PX-lines.txt *F the format for each P-element line in the above file is as follows: *F and I have just copied the line relevant to each particular P-insertion *F in the questions I have that follow. I am sorry there are several *F questions, I have tried to organise them into similar categories to *F make it easier. Any extra information you can give me about these *F lines would be very useful and I would record the information as a *F personal communication from you to FlyBase. *F 1. viable lines. *F The following lines are listed as viable in the supplementary *F information, but we already have a record of them in FlyBase as lethal *F genes \+ alleles. *F My question for these is, in each case, is there any lethality in the *F strain or are the flies homozygous viable ? If the flies are homozygous *F viable I will remove the lethal alleles from FlyBase and will just make *F insertion records to store the information like the accession number *F and the affected gene. *F If there is some homozygous lethality in the strain, does this mean *F that in each case the lethality is separable from the insertion ? *F The lines are: *F l(1)G0500,AJ300032,12282,2C2,2C1-2,east,viable *F l(1)G0123,AJ426927,11885,13E3-8,13E1-6,sog,viable *F l(1)G0104,AJ427038,11817,,12D,yoyo,viable *F l(1)G0398,AJ427047,11909,,12C,yoyo,viable *F l(1)G0250,AJ426807,11925,8D10-12,8E1-8,CG17251,viable *F l(1)G0346,AJ426808,11929,8D10-12,8E1-4,CG17251,viable *F l(1)G0400,AJ426812,12006,8F3-5,(3A1-4 &) 8F,CG15321,viable *F l(1)G0230,AJ426821,11955,9B6-7,9B1-8,CG2968,viable *F l(1)G0444,AJ426867,12049,10C4-5,10C1-2,CG1558,viable *F l(1)G0185,AJ426901,12153,12D4-E1,12E5-7,CG11595,viable *F l(1)G0225,AJ427021,12231,18F1-2,18F,CG12701,viable *F l(1)G0094a,AJ426682,11811,4C8,4D1-2,ctp,viable *F l(1)G0094b,AJ426841,11811,9E2-4,9E3-4,ras,viable *F 2. discrepancies between cytology and sequence localisation *F a. In the following lines there is a discrepancy between the *F 'LOCALIZATION' (determined molecularly) and the 'IN SITU HYBRIDIZATION' *F localisation. *F In each case, could you tell me whether you think that this means that *F there are actually 2 inserts \- one defined by the accession number and *F one defined by the in situ hybridisation, or whether you think there is *F just one insertion and there is an error in the in situ value. *F The lines are: *F l(1)G0031,AJ299996,,1B5-6,12C,elav,E *F l(1)G0024,AJ300007,11496,1D1,3B,BACR7A4.15,L3 *F l(1)G0015,AJ300074,,3C7,2C7-8,N,E *F l(1)G0449,AJ426694,10104,5C5-6,4D4-6,Act5C,'L3, short L3s' *F l(1)G0182,AJ426748,11950,7A4,12A1-2,brk,E *F l(1)G0327,AJ426757,12292,7C8-D1,1B7-10,CG2206,L1-L2 *F l(1)G0238,AJ426839,11957,9E2-4,7E3-6,ras,fl *F l(1)G0141,AJ426868,10148,10C6-7,11A7-8,CG1703,'L3-pP-P-pA-A, short bristles, ms' *F l(1)G0476,AJ426910,10127,12D4-E1,13E1-4,CG1405,L3-pP *F l(1)G0285,AJ426999,11965,18D8-11,19E,CG12238,fs *F l(1)G0196,AJ427035,11872,19F6-20A1,9E1-4,CG14616,L1-L2 *F l(1)G0270,AJ426818,11873,8F3-5,9E4-10,CG15321,P-pA *F l(1)G0473,AJ426952,10125,14B9-12,11B1-4,CG3415/katanin-80,fs *F b. the following 2 lines also show a discrepancy between the cytology *F and sequence localisation, and in addition they are also listed as *F viable: *F l(1)G0495,AJ426760,12280,7D5-6,12C,fs(1)h,viable *F l(1)G0060,AJ426693,11653,5B2-5,11B1,CG3125,viable *F for these, could you tell me whether you think that this means that *F there are actually 2 inserts \- one defined by the accession number and *F one defined by the in situ hybridisation, or whether you think there is *F just one insertion and there is an error in the in situ value. In *F addition, are these lines homozygous viable or is there a separable *F lethality somewhere in the strain (like question 1.) *F 3. l(1)G0459,AJ427049,12260,,3A & 10E-F,yoyo,fs *F By BLAST, the AJ427049 sequence given for this line is in the gene *F c12.1 and is identical to the sequence given in accession record *F AJ426806 \- is the sequence in AJ427049 a mistake ? *F here is the sequence I got from EMBL for AJ427049: *F >embl|AJ427049|DME427049 Drosophila melanogaster X chromosomal sequences *F flanking P-lacW insertion, strain l(1)G0459 *F gtactgttgatacatcaaacaaagcacgcagttcggaaattcatttctcttttcattaat *F ttaacaattaaacgcataactagctagttgaaaatgaccacagcagcgc *F Also, in a previous personal communication to FlyBase (FBrf0125103, *F date:1999.12.2) you gave the in situ localisation for l(1)G0459 as 7D *F Could you tell me which in situ localisation is correct \- 3A & 10E-F as *F given in the supplementary info, or 7D as in FBrf0125103. *F 4. l(1)G0396,AJ426858,,10B8,10B1-2,KAP3,'pA, die at hatching' *F I just wanted to check that the 'KAP3' mentioned in your abstracts: *F Peter et al., 1999, Europ. Dros. Res. Conf. 16: 264 *F 'Characterization of a gene encoding the kinesin associated protein 3 *F (KAP3) in Drosophila melanogaster.' *F and *F Pflanz et al., 2001, Europ. Dros. Res. Conf. 17: O4 *F 'Functional analysis of the kinesin-associated protein KAP3.' *F is the same gene as the one affected in the l(1)G0396 strain. *F 5. *F l(1)G0252,AJ426889,11880,11D5-6,11C,lic,'semilethal, m & f' *F The sequence of AJ426889 at EMBL is identical to AJ426848 and does not *F map to lic in 11C but maps by BLAST to region 9 cytologically in *F sesB/Ant2 \- has there been a mistake in the sequence in AJ426889 ? *F here is the sequence I got from EMBL of AJ426889: *F >embl|AJ426889|DME426889 Drosophila melanogaster X chromosomal sequences *F flanking P-lacW insertion, strain l(1)G0252 *F atagcccatttaacaaaanacgngngtggttccaaangcttttattaatanttttttttt *F ttaaatcaaatccaacctgagcattgaaagaaaatctgcctggttatgtaaaacaacaaa *F agagttgaacttgaatcattcattgaacaattttttttntggtgtgtggtggtggtctta *F tgcaatcaatacncaatgaattaagaataatcttaacactggattgtggatacanctaag *F gctgttcagccttcggtggaagctttccacgtgcaactgtagaatttttccagctgaggc *F tattacatcacgagccntttagctatnggctctntgnggctgtggccgaagaaaaagctt *F cactctctttccagccaatcaaaagtctaacacgattagataacggttgttatttcaaag *F aaagagaacanaagagnagcgaancaatggcaaagcccagttctctttgccaagtgtgtt *F acataaaacccttgtcaatgaaatgctgatttaatttcgctaaaatattttttaaaccac *F gcggctaacgcagagcggcggcatgctcttgaaagtctaaaattaacaatacgcgc *F 6. the sequence in the two accession number AJ427025 (l(1)G0004 ) and *F AJ427026 (l(1)G0005) are identical \- is this correct ? *F here are the sequences I got from EMBL: *F >embl|AJ427025|DME427025 Drosophila melanogaster X chromosomal sequences *F flanking P-lacW insertion, strain l(1)G0004 *F gatcccagcacctggccctcaatgccggtcgcctcatccacctgcatctccacgtcctgc *F tgcccgcctccgctggctccgcgcttcgtcgctctcttcgccggctcgaatgcgtctgct *F c *F >embl|AJ427026|DME427026 Drosophila melanogaster X chromosomal sequences *F flanking P-lacW insertion, strain l(1)G0005 *F gatcccagcacctggccctcaatgccggtcgcctcatccacctgcatctccacgtcctgc *F tgcccgcctccgctggctccgcgcttcgtcgctctcttcgccggctcgaatgcgtctgct *F c *F 7. l(1)G0001,,,,nd (P-GawB!),yoyo,E *F I just wanted to check that this strain contains a GawB insertion *F instead of a lacW ?! *F 8. *F l(1)G0460a,AJ300082,,3C7,nd,N,E *F l(1)G0460b,AJ426801,,8B4-7,8B3-7,Moe,E *F the sequences given in AJ300082 and AJ426801 are identical and both map *F to Moe in 8B. Is there are mistake with AJ300082 as it is said to map *F to 3C in N ? *F here are the sequences I got from EMBL: *F >embl|AJ300082|DME300082 Drosophila melanogaster X chromosomal sequence *F flanking P-lacW insertion strain l(1)G0460a *F gcgtcggcttttcatttttcgttcatttatttattttcctctccagtttttttttttttt *F tttgggtgcaacaagtgtgcggcgctttgttgtttttgtaccgttttttatgttaattaa *F ccaaaacgagttgttgttcgtgtgtttgctatgcttgactagttaagcgcgtagagagca *F aaagagatggagagggagcgaacgaaagagagaggaagaaagatcattacgtaatgggga *F aaacgttgctttcaaattctccagtgttgccaccttatcaaaatgacacttgttacgctc *F cgcgacccctttttaacccttttccattgcttatttaaaaatttaaattaaacatttttt *F atgaaaatttcaaaagttttcctattcacctgtttttttagtggtcaaaacattttatgc *F caacgtgaattggcaatactggttgttaagccgcgtgactcgtttttttttttgaccgca *F cataaaattcaaatttttgttgtttgtttaccgatcatcttgcattctctcgctcgcttt *F tatttctctctgtttgcatttcgttttgactccttctttggccttactcatttgatttcg *F gacgcttgagacgtgcgcttttcgcagcaatcgcatcgaaagaagagagcaaggagttgc *F gagggagagatgaagtgaccgaaagagagagtcaaaagagagtagacttttcttctagaa *F accacgatgccttttaataaatcatagttttccaagtgagtaattttaaccccgccttca *F cccaattattcaaatgcagcggngccgagaaaaaattatattgtnaatgccatgggtcaa *F aaaaaaaagctgc *F >embl|AJ426801|DME426801 Drosophila melanogaster X chromosomal sequences *F flanking P-lacW insertion, strain l(1)G0460b *F gcgtcggcttttcatttttcgttcatttatttattttcctctccagtttttttttttttt *F tttgggtgcaacaagtgtgcggcgctttgttgtttttgtaccgttttttatgttaattaa *F ccaaaacgagttgttgttcgtgtgtttgctatgcttgactagttaagcgcgtagagagca *F aaagagatggagagggagcgaacgaaagagagaggaagaaagatcattacgtaatgggga *F aaacgttgctttcaaattctccagtgttgccaccttatcaaaatgacacttgttacgctc *F cgcgacccctttttaacccttttccattgcttatttaaaaatttaaattaaacatttttt *F atgaaaatttcaaaagttttcctattcacctgtttttttagtggtcaaaacattttatgc *F caacgtgaattggcaatactggttgttaagccgcgtgactcgtttttttttttgaccgca *F cataaaattcaaatttttgttgtttgtttaccgatcatcttgcattctctcgctcgcttt *F tatttctctctgtttgcatttcgttttgactccttctttggccttactcatttgatttcg *F gacgcttgagacgtgcgcttttcgcagcaatcgcatcgaaagaagagagcaaggagttgc *F gagggagagatgaagtgaccgaaagagagagtcaaaagagagtagacttttcttctagaa *F accacgatgccttttaataaatcatagttttccaagtgagtaattttaaccccgccttca *F cccaattattcaaatgcagcggngccgagaaaaaattatattgtnaatgccatgggtcaa *F aaaaaaaagctgc *F that's it, sorry there are so many questions \! *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Thu, 4 Dec 2003 11:33:07 \+0100 *F To: Gillian Millburn (Genetics) *F From: Ulrich Schaefer *F Subject: Re: FlyBase query: Gottingen P-element lines *F Dear Gillian, *F thank you for your e-mail and particularly for the effort in sieving *F through our data on those lethal lines. ... I will go through all your *F questions in the order you mailed me and I will answer them and *F correct any errors. *F 1. *F The P insertion lines were kept if the insertion caused lethality *F (few escapers <1% compared to their FM7c brothers that were already *F clearly affected in their viability) or semilethality (<20% of FM7c *F brothers). I did a quantitative recheck of the lethal effect after *F the final establishing of the lines. In addition, Peter Deak in David *F Glover's lab screened our lines for the timepoint of lethality and his *F data are incorporated in the table. The label 'viable' just points *F out that adult flies eclosed in his analysis even when I could find *F none or only a few escapers as listed below. *F l(1)G0500: semilethal (<20% of FM7c control) *F l(1)G0123: semilethal (<10% of FM7c control) *F l(1)G0104: semilethal (<10% of FM7c control) *F l(1)G0398: semilethal (<5% of FM7c control) *F l(1)G0250: semilethal (<10% of FM7c control) *F l(1)G0346: semilethal (<5% of FM7c control) *F l(1)G0400: lethal (<1% of FM7c control), no escapers in my hands *F l(1)G0230: lethal (<1% of FM7c control), no escapers in my hands *F l(1)G0444: semilethal (<20% of FM7c control) *F l(1)G0185: lethal (<1% of FM7c control), no escapers in my hands *F l(1)G0225: lethal (<1% of FM7c control) *F l(1)G0094a,l(1)G0094b: semiviable (<50% of FM7c control), faint *F bristles and female sterile *F In addition, I should point out that Timothy Karr (University of *F Bath) has detected Wolbachia in most of our strains and that the *F strength of this bacterial infection might influence the vitality of *F the flies. *F 2. *F a) Although there is no final proof available we have no evidence for *F another site as indicated by the in situ hybridisation. Hence, *F everything is in favor that the molecular localisation is correct and *F that the in situs are misinterpreted. *F b) Same answer as in 2a). With regard to the viability here are my *F data (also seen in the background of 1) *F l(1)G0495: lethal (<1% of FM7c control) *F l(1)G0060: lethal (<1% of FM7c control), no escapers in my hands *F 3. *F My fault. I did submit the same sequence twice as you suggested. The *F correct sequence for l(1)G0459 follows. It is derived from 5' and 3' *F inverse PCR and has the P insertion at position 11 (i.e. first *F position of 8 bp duplication). *F GCGCTGGTGA GTCCTGACGA AGATCGCAAG AAGAGGGTTC GTAACTTACA CGAACATGTT *F ACTAGACAGG CCCCGAGTCG CCTGCTGACG GGGCCCGAAA ACACGAGAGC CGAGCGC *F 4. *F Your assumption is correct, the l(1)G0396 strain is mutated in the KAP3 gene. *F 5. *F My fault again. The correct sequence for l(1)G0252 follows. It is *F derived from 5' inverse PCR and has the P insertion at position 119 *F (i.e. first position of 8 bp duplication). *F CCGGGTTTTG CCGCCCAACC TTTCGCTATT GCCTTTGCTT TCCTTTCCGC ACTTTTTGCA *F CTGTTCACCG ATCGCTTTTC GCTTCTCTTC TCGTTTTTCT CTCTTTTCCA CTAGCGCTGG *F CCAGCC *F 6. *F Yes, both lines gave the same insertion sequences. They were *F originally isolated from different remobilisation vials. I can, *F however, not exclude that \- likely \- an error occurred during the *F passages until we determined the insertion site. *F 7. *F Yes, our only line with a GawB insertion. *F 8. *F It's my fault again. The correct sequence for l(1)G0460a follows. It *F is derived from 3' plasmid rescue and has, therefore, the P insertion *F at position 1 (i.e. first position of 8 bp duplication). *F GAGGAGGCAA TGAAAAATGA CAATCATCAT TATATACACT TGAGCGGATC GACGACAGTC *F GCCGATCGTG TGCTCGTCGT CTTCTCAAGT TGAAACTGAA ATTGCTACGA CTTTGTCGTC *F TCTTCCTTCT TCTCCGCCGA CATCTCCTTG TCTTTTTCAG TCTCCGTCTC CATCTTTATA *F TTTTTCTCTT CACATGCGTG TTTGAGAATC ATCTGGGGGA GAGGGAGAAT AGAGATCGGT *F CCAAGCTCAA GTGAGTGGTT TGACTTTAAG AGATGCATAG ATACTTTGGC TTGCATAATA *F ATAATAATAA TAATAGTAAT GATCGCTGGT CGAAGGAAGA AGCCATGCAG AAAGATACGG *F GACGATATGA TGGCGAATGA TACTCTTTCA TGGTGGTCTT GGGAGTTTTA GAATCATTAC *F AGATACCCTC ACTCAAAACA TAAGCAAAGA TAAAAGAATT AATTGCAAAT TTGACAGATA *F TGGGTTATAT AACTATAAAT TGGAAAACTC ACGTACAGCT AAAGGTTAGA TCATTCTAAA *F GGTAACTGGA TACCCTGATA *F GATCCTTAAG ATAAATAAAA GGCACCTTCG AAACTGCAGG ATACACAAAC ACTGAGATAA *F ACACATGCTT CAAGCTGCCA CTTGAAAATG TATCTCAAAT TCAAGCGTCT GACACTCGCT *F GGCTCTATAA AGTCTTAAGA TGAGAATCTT GAAGGAAATA TGTAAGAAGG ATGTCTCTGT *F TTTGGCTTGC GATTCCCAAC GAACGATTTG AAGTTTAACT ATAAATATAA GATACCTAAG *F TTTCGATTTA GGTACATATG TACATATCTG GAATTAAAGT CCAGTTTTTT TATGGCTGCA *F TGATTGTAGC AACTGCGTTC ACTTCATCTG ACGCTCACTT AGCATTGATT AGTAGCCATA *F TCTCGCGGAT GCATCTTTCT CTTTGACTTT AGCCCTAAG *F ... *F I hope the information I provided is sufficient for you. ... *F regards *F Ulrich *F \-- *F Dr. Ulrich Schaefer *F Max-Planck-Institut fuer biophysikalische Chemie *F Abt. Molekulare Entwicklungsbiologie *F 37070 Goettingen *F GERMANY *F Street address: Am Fassberg 11, 37077 Goettingen *F Phone: (+49) 551 201-1798 *F Fax: (+49) 551 201-1755 *F __ *F (oo) Have a look at the web page of the department! *F /( )\ URL: http://www.mpibpc.gwdg.de/abteilungen/170/ *F ^^ # *U FBrf0173187 *a Smith *b D. *t 2003.12.15 *T personal communication to FlyBase *u *F To: xwang@biochem.swmed.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 9 Dec 2003 17:57:54 \+0000 *F Dear Dr. Wang, *F I am curating your paper for FlyBase: *F Liu et al., 2003, Science 301(5641): 1921--1925 *F and I have a couple of questions about the r2d2 mutant shown in Figure *F 2D and E. *F 1. You call the starting P-element strain 'P-2450' \- is this a stock *F number ? does the strain have any other form of identifier \- I am *F trying to work out which P-element strain in FlyBase it corresponds *F to. *F 2. do you have a symbol you are using for the r2d2 mutant e.g. r2d21 *F ( = superscript) \- I need to give it a symbol that includes something *F in superscript so that it can be distinguished from other r2d2 mutants *F when they are made, *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F To: gm119@gen.cam.ac.uk *F From: Dean Smith *F Date: Mon, 15 Dec 2003 11:09:26 \-0600 *F The starting R2D2 mutant P element was EP2450, located upstream of r2d2 *F (CG7138). *F The mutant is by convention, r2d21 with r2d2 in italics and 1 *F denoting superscript. *F Thanks, *F Dean Smith *F >> I am curating your paper for FlyBase: *F >> *F >> Liu et al., 2003, Science 301(5641): 1921--1925 *F >> *F >> and I have a couple of questions about the r2d2 mutant shown in Figure *F >> 2D and E. *F >> *F >> 1. You call the starting P-element strain 'P-2450' \- is this a stock *F >> number ? does the strain have any other form of identifier \- I am *F >> trying to work out which P-element strain in FlyBase it corresponds *F >> to. *F >> *F >> 2. do you have a symbol you are using for the r2d2 mutant e.g. r2d21 *F >> ( = superscript) \- I need to give it a symbol that includes *F >> something *F >> in superscript so that it can be distinguished from other r2d2 mutants *F >> when they are made, # *U FBrf0173192 *a Schaefer *b U. *t 2004.1.8 *T personal communication to FlyBase *u *F To: uschaef@gwdg.de *F Subject: FlyBase query: Gottingen P-element lines *F From: Gillian Millburn (Genetics) *F Date: Thu, 8 Jan 2004 11:40:02 \+0000 *F Dear Ulrich, *F I am doing a final check on my curation of the supplementary *F information from your paper Peter et al., 2002, EMBO Rpts 3(1): 34--38 *F about the Gottingen lines, and I realise I forgot to ask you about one *F particular line \- sorry. *F The line in question is: *F l(1)G0462,AJ426758,,7C8-D1,7D1-3,CG2206,L1-L2 *F so the data from the EMBO Rpts info suggests that this line is a single *F insert line, with the insert mapping to 7C-7D. *F However, we have a personal communication from you from 14th December *F 1999, in which you stated that this line was a multiple insert line, it *F is FBrf0125056. *F here is relevant bit of the pc: *F Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun *F He, Hugo Bellen, Todd Laverty and Gerry Rubin *F To: Bloomington Drosophila Stock Center *F Subject: Goettingen lethals \- set 6 *F Dated: 14 December 1999 *F New Alleles (presumed to be caused by P{lacW} insertions): *F l(1)G0462G0462 ? \-- multi-insert line: 3A, 10E-F *F New Transposon insertions (from multi-insert lines): *F P{lacW}G0462a 3A *F P{lacW}G0462b 10E-F *F can I assume that the more recent data is correct and that you assume *F that there is actually only one insert in this line at 7C-7D ? *F sorry I forgot to ask you before, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Thu, 8 Jan 2004 18:26:50 \+0100 *F To: Gillian Millburn (Genetics) *F From: Ulrich Schaefer *F Subject: Re: FlyBase query: Gottingen P-element lines *F Dear Gillian, *F line l(1)G0462 is mapped by in situ to 7D1-3 and thus in accord with *F the molecular data. The only line I have that is mapped by in situ to *F 3a & 10E-F is line l(1)G0466. The best explanation for the *F FBrf0125056 note is, therefore, that a typo \- .. *F \- caused the error. By the way, the latter line is one of the more *F than ten lines where we could never substantiate the second in situ *F site at 3A whereas the other \- variable \- site was always verified *F molecularly. Hence, we are quite confident that the second 3A site is *F an artifact. *F .. *F Ulrich *F \-- *F Dr. Ulrich Schaefer *F Max-Planck-Institut fuer biophysikalische Chemie *F Abt. Molekulare Entwicklungsbiologie *F 37070 Goettingen *F GERMANY *F Street address: Am Fassberg 11, 37077 Goettingen *F Phone: (+49) 551 201-1798 *F Fax: (+49) 551 201-1755 *F __ *F (oo) Have a look at the web page of the department! *F /( )\ URL: http://www.mpibpc.gwdg.de/abteilungen/170/ *F ^^ # *U FBrf0173216 *a Kpebe *b A. *c L. *d Rabinow *t 2004.1.15 *T personal communication to FlyBase *u FlyBase error report for CG31049. *F From: FlyBase-error@ogre.lbl.gov *F Date: Tue, 18 Nov 2003 08:12:39 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: Leonard.Rabinow@emex.u-psud.fr *F Subject: FlyBase error report for CG31049 on Tue Nov 18 08:12:39 2003 *F Error report from Leonard Rabinow (Leonard.Rabinow@emex.u-psud.fr) *F Gene or accession: CG31049 *F Release: 3 *F cDNA or EST error *F Comments: As we published in 2000, multiple DOA proteins exist (we're up to *F 4), based upon data from western blots, confirmed with affinity-purified *F antibodies and use of mutants to confirm origin of the protein. We sequenced *F RE30665 in its entirety- this EST contains the N-terminal end of CG31049 and *F the C-terminal (Kinase domain) of Doa, CG1658, as we'd hoped, and thus it *F encodes one of the alternative Doa isoforms (of 105 kD). *F Given its placement in the gene, I suspect that CG33204 will also turn out to *F be a Doa isoform, but we've not done anything with that yet, nor do we have *F any experimental evidence to confirm it actually exists. Do you have any I'm *F missing in the database? *F Also, please note that a previous BLAST analysis we reported *F (Rabinow and Rabinow, 2001.5.23 *F Personal communication to FlyBase available from *F http://flybase.bio.indiana.edu/.bin/fbpcq.html?FBrf0135387  FBrf0135387) *F with sequence from one of the alternative cDNAs we'd obtained was partly *F misleading and should be deleted from the database if possible. *F Thank you very much. *F Best wishes, *F Lenny Rabinow *F Date: Thu, 15 Jan 2004 10:47:41 \+0100 *F To: Beverley Matthews *F From: Leonard Rabinow *F Subject: Re: Doa/CG31049 correction *F Cc: arlette.kpebe@ibaic.u-psud.fr *F >Dear Dr. Rabinow, *F > *F >It would be very helpful if you could send a FASTA file of RE30665 and the *F >encoded CDS to insure that we properly correct the Doa annotation. If you can *F >send it soon, I will try to include it in the upcoming annotation release. *F >Thanks for your help. *F > *F >Sincerely, *F > *F >Beverley Matthews *F >Curator, FlyBase-Harvard *F > *F > *F Hello Beverly, *F .. *F Attached is a Word file (sorry for the improper format, we'll do better *F next time) of the complete DNA sequence of RE30665, supporting the fact *F that CG31049 is actually an alternately spliced (and initiated) isoform of *F Doa kinase (CG1658). Based on northern hybridizations and predicted protein *F size from the ORF, this message, reported in Yun et al 1994 as 4.0 kb *F FBrf0074875 *F Yun, B., R. Farkas, K. Lee, and L. Rabinow. 1994. The Doa locus encodes a *F member of a new protein kinase family, and is essential for eye and *F embryonic development in Drosophila melanogaster. Genes Dev. 8: 1160-1173 *F encodes the 105 kD cytoplasmic isoform of the kinase reported in Yun et al. *F 2000 *F FBrf0130184 *F Yun, B., K. Lee, R. Farkas, C. Hitte, and L. Rabinow. 2000. The LAMMER *F protein kinase encoded by the Doa locus of Drosophila is required in both *F somatic and germ-line cells, and is expressed as both nuclear and *F cytoplasmic isoforms throughout development. Genetics 156: 749-761. *F If you attribute this data, please cite A. Kpebe and L. Rabinow, *F unpublished (or in prep.) Thanks. *F As for another predicted gene in the introns of Doa, CG33204, we've no *F evidence either way regarding it. I'm willing to bet it's yet another DOA *F isoform. I note that the ESTs supporting this 'gene': *F RH25104; RH05514; RH28293; RH21287; RH06871; RH31228; RH70859 *F are all 5' sequences. I will order one or two of the above ESTs and we'll *F sequence from the 3' side (unless you can request the folks at the *F physical-end of BDGP to it first, since I'm sure they'll be quicker). Let *F me know if this is of any interest to you. *F Any insight into any of the above would be appreciated, With all the *F information available through Flybase it's easily possible I'm missing yet *F other annotations of interest to us on Doa. *F Thanks very much for your assistance. *F Best wishes, *F Lenny Rabinow *F \-------------------------------------------------------------------- *F Leonard Rabinow *F Signalisation, Developpement et Cancer *F Batiment 442 bis *F Universite Paris XI *F 91405 ORSAY CEDEX *F FRANCE *F Leonard.Rabinow@emex.u-psud.fr *F http://www.u-psud.fr/orsay/recherche/ibaic/SDC.nsf/SDC201.htm!OpenPage *F Phone: 1-69-15-74-65 *F FAX: 1-69-15-68-02 *F (from US, prefix: 011-33) *F File deposited: Kpebe.2004.1.15.doc ; File date: 2004.2.3 ; File size: 38912 ; *F File format: doc # *U FBrf0173217 *a Mitchell *b P.J. *t 2004.1.20 *T personal communication to FlyBase *u *F Date: Tue, 20 Jan 2004 18:39:39 \-0500 *F To: Beverley Matthews *F From: 'Pamela J. Mitchell' *F Subject: Re: AP-2 mutations *F Dear Beverly, *F Your guesses were correct! *F Thanks, *F Pam Mitchell *F >Dear Dr. Mitchell, *F > *F >I am a curator for FlyBase and am annotating the molecular basis of the *F >AP-2 mutations from your 2001 Monge et. al. Development paper. This *F >type of information should start appearing soon in the annotation *F >reports. I didn't have quite enough information to locate the sites of *F >the AP-29 (GG to AG change creating a new splice acceptor in intron *F >2) and AP-23 (TG to AG change in intron 1, creating a new splice *F >acceptor) mutations. *F > *F >Could you send me some sequence surrounding the site of the mutations *F >so I can locate them accurately in the genome? *F > *F >For AP-29, I am guessing that it is the most 3' GG in intron 2 *F > *F >wt AGATATCC GG CTACTTGCA *F >mutant AGATATCC AG CTACTTGCA *F > *F >For AP-23, is it also the one closest to the splice acceptor? *F > *F >wt TTCCGCT TG TCTTCCCC *F >mutant TTCCGCT AG TCTTCCCC *F > *F >Thanks for your assistance, *F > *F >Sincerely, *F >Beverley Matthews *F >FlyBase-Harvard *F Pamela J. Mitchell, Ph.D. *F Associate Professor *F Department of Biochemistry & Molecular Biology *F Pennsylvania State University *F 332 South Frear *F University Park, PA 16802 *F Phone: (814) 865-5802 (office) or \-5803 (lab) *F FAX: (814) 863-7024 *F email: pjm23@psu.edu *F http://www.bmb.psu.edu/faculty/mitchell/mitchell.html # *U FBrf0173218 *a Heidmann *b S. *t 2003.11.11 *T personal communication to FlyBase *u FlyBase error report for CG5785 on Tue Nov 11 08:23:43 2003. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Tue, 11 Nov 2003 08:23:43 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: stefan.heidmann@uni-bayreuth.de *F Subject: FlyBase error report for CG5785 on Tue Nov 11 08:23:43 2003 *F Error report from Stefan Heidmann (stefan.heidmann@uni-bayreuth.de) *F Gene or accession: CG5785 *F Gene annotation error *F I have new supporting evidence or functional assignment for gene CG5785. *F Comments: Dear Madam or Sir, *F I would like to present evidence that the protein product encoded by the gene *F CG5785 (thr) is larger at its N-terminus than appears in FlyBase. *F The FlyBase (FBpp0001383) and the GenBank entries (AAB60210) correspond to a *F D. melanogaster THR protein of 1209 aa in length. In fact, the thr reading frame *F can be extended in the 5' direction, so that a protein of 1379 aa is encoded. *F There is a full length EST entry (SD03660, accession number AY128502) that *F supports this extension. Unfortunately, this sequence contains a frame shift, *F thus resulting again in a protein that is too small. There is a run of seven *F adenosines at nt positions 2045-2051 of AY128502. The deletion of one adenosine *F would correct the frame shift, which then results in an ORF long enough to *F encode a protein of 1379 aa. This deviation in the length of the adenosine track *F is obvious when performing alignments using AY128502. All genomic sequences as *F well as the ESTs LP01826 (accession no. AI258595) and LD22604 (accession no. *F AA817348) have six instead of seven adenosines at the respective position. *F Indeed, when using the FlyBase Apollo Genome Annotation tool, the SD03660 *F sequence that can be extracted has the coding potential for a 1379 aa protein. *F At last but not at least, we have experimental data supporting a 1379 aa long D. *F melanogaster THR protein: First, western blot analyses reveal that the *F endogenous THR protein runs at a higher molecular weight than the 1209 aa *F version expressed from a transgene. Second, the heat shock induced expression of *F the 1379 aa version of THR rescues the thr mutant phenotype in the embryo, while *F the same experiment with the 1209 aa version failed to rescue. Also, we have *F recently undertaken a comparative analysis of thr genes from various Drosophila *F species (Jaeger et al. Structure Predictions and Interaction Studies Indicate *F Homology of Separase N-Terminal Regulatory Domains and Drosophila THR. Cell *F Cycle 2004. in press). The similarity, particularly when the N-terminal *F extension of D. melanogaster THR is compared with the N-terminal region of the *F proposed D. pseudoobscura THR protein (AY352649) suggests functional *F conservation of these sequences. Finally, we have produced a series of genomic *F THR deletion *F constructs fused at their C-terminus with an epitope tag (Jaeger et al. *F Drosophila *F separase is required for sister chromatid separation and binds to PIM and THR. *F Genes Dev. 2001. 15:2572-2584). The migration behaviour in a gel of those *F epitope-tagged products containing the THR N-terminus is consistent with an *F N-terminally extended, 1379 aa version of THR. *F Taken together, all our data clearly support a 1379 aa protein encoded by *F the D. melanogaster thr gene. Therefore, we believe that the annotation of thr *F in Flybase should be corrected to take these observations into account. *F Please don't hesitate to contact me in case you need further information. *F Sincerely, *F Stefan Heidmann # *U FBrf0173219 *a Wainman *b A. *t 2003.11.7 *T personal communication to FlyBase *u FlyBase error report for CG6875 on Fri Nov 7 10:06:22 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Fri, 7 Nov 2003 10:06:23 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: ajw64@hermes.cam.ac.uk *F Subject: FlyBase error report for CG6875 on Fri Nov 7 10:06:22 2003 *F Error report from alan (ajw64@hermes.cam.ac.uk) *F Gene or accession: CG6875 *F Release: 3 *F Gene annotation error *F Gene CG6875 has incorrect exon/intron structure or translation start site. *F Protein sequence: *F >MELVWSPVLEVACKETLQLIDNRNFRKEVMIILKSKSNQPVKNPRKFPTVGKTLQLKSPTGAGKTMKSVVSAAVQQKK *F RMSAAAAPPSKQTWRVTAPSRPAAWAHPPPQAPLVEKNVYKTPQEEPVYISPQPRSLKENLSPMTPGNLLDVIDNLRFT *F PLTETRGKGQATIFPDNLAAWPTPTLKGNVKSCANDMRPRRITPDDLEDQPATNKTFDVKHSETINISLDTLDCSRIDG *F QPHTPLNKTTTIVHATHTRALACIHEEEGPSPPRTPTKSAIHDLKRDIKLVGSPLRKYSESMKDLSLLSPQTKYAIQGS *F MPNLNEMKIRSIEQNRYYQEQQIQIKAKDLNSSSSSEASLAGQQEFLFNHSEILAQSSRFNLHEVGRKSVKGSPVKNPH *F KRRSHELSFSDAPSNESLYRNETVAISPPKKQRVEDTTLPRSAAPANASARSSSAHAWPHAQSKKFKLAQTMSLMKKPA *F TPRKVRDTSIQPSVKLYDSELYMQTCINPDPFAATTTIDPFLASTMYLDEQAVDRHQADFKKWLNALVSIPADLDADLN *F NKIDVGKLFNEVRNKELVVAPTKEEQSMNYLTKYRLETLRKAAVELFFSEQMRLPCSKVAVYVNKQALRIRSDRNLHLD *F VVMQRTILELLLCFNPLWLRLGLEVVFGEKIQMQSNRDIVGLSTFILNRLFRNKCEEQRYSKAYTLTEEYAETIKKHSL *F QKILFLLLFLDQAKQKRIVKHNPCLFVKKSPHKETKDILLRFSSELLANIGDITRELRRLGYVLQHRQTFLDEFDYAFN *F NLAVDLRDGVRLTRVMEVILLRDDLTRQLRVPAISRLQRIFNVKLALGALGEANFQLGGDIAAQDIVDGHREKTLSLLW *F QLIYKFRSPKFHAAATVLQKWWRRHWLHVVIQRRIRHKELMRRHRAATVIQAVFRGHQMRKYVKLFKTERTQAAIILQK *F FTRRYLAQKQLYQSYHSIITIQRWWRAQQLGRQHRQRFVEL! *F REAAIFLQRIWRRRLFAKKLLAAAETARLQRSQKQQAAASYIQMQWRSYQLGRIQRQQFLRQRDLIMFVQRRMRSKWS *F MLEQRKEFQQLKRAAINIQQRWRAKLSMRKCNADYLALRSSVLKVQAYRKATIQMRIDRNHYYSLRKNVICLQQRLRAI *F MKMREQRENYLRLRNASILVQKRYRMRQQMIQDRNAYLRTRKCIINVQRRWRATLQMRRERKNYLHLQTTTKRIQIKFR *F AKREMKKQRAEFLQLKKVTLVVQKRRRALLQMRKERQEYLHLREVTIKLQRRFHAQKSMRFMRAKYRGTQAAVSCLQMH *F WRNHLLRKRERNSFLQLRQAAITLQRRYRARLNMIKQLKSYAQLKQAAITIQTRYRAKKAMQKQVVLYQKQREAIIKVQ *F RRYRGNLEMRKQIEVYQKQRQAVIRLQKWWRSIRDMRLCKAGYRRIRLSSLSIQRKWRATVQARRQREIFLSTIRKVRL *F MQAFIRATLLMRQQRREFEMKRRAAVVIQRRFRARCAMLKARQDYQLIQSSVILVQRKFRANRSMKQARQEFVQLRTIA *F VHLQQKFRGKRLMIEQRNCFQLLRCSMPGFQARARGFMARKRFQALMTPEMMDLIRQKRAAKVIQRYWRGYLIRRRQKH *F QGLLDIRKRIAQLRQEAKAVNSVRCKVQEAVRFLRGRFIASDALAVLSRLDRLSRTVPHLLMWCSEFMSTFCYGIMAQA *F IRSEVDKQLIERCSRIILNLARYNSTTVNTFQEGGLVTIAQMLLRWCDKDSEIFNTLCTLIWVFAHCPKKRKIIHDYMT *F NPEAIYMVRETKKLVARKEKMKQNARKPPPMTSGRYKSQKINFTPCSLPSLEPDFGIIRYSPYTFISSVYAFDTILCKL *F QIDMF *F Comments: the asp protein encoded by the supposed 'full-length' cDNA, *F LD39479.pep is shorter than that published by Saunders et al. 1997. # *U FBrf0173220 *a Torroja *b L. *t 2003.10.29 *T personal communication to FlyBase *u FlyBase error report for CG18853 on Wed Oct 29 08:59:30 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Wed, 29 Oct 2003 08:59:30 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: laura.torroja@uam.es *F Subject: FlyBase error report for CG18853 on Wed Oct 29 08:59:30 2003 *F Error report from Laura Torroja (laura.torroja@uam.es) *F Gene or accession: CG18853 *F Release: 3 *F Assembly error *F Comments: sequence CG18853 aligns (exact match) with more than one stretch of *F genomic DNA in AE003839: sequence 1-797 of CG18853 transcript aligns with *F AE003839 sequence 40608-41405 (gene CG18853) and 43718-44514 (gene CG12822); *F sequence 793-1341 of CG18853 transcript aligns with AE003839 sequence *F 38290-38838(gene phr) and 41401-41948(gene CG18853). These 3 genes should also *F be checked(CG18853 appears to be a hybrid of phr and CG12822, but I have not *F analyzed all the information). *F Date: Wed, 29 Oct 2003 12:37:08 \-0500 (EST) *F From: Madeline Crosby *F Subject: Re: FlyBase error report for CG18853 on Wed Oct 29 08:59:30 2003 *F To: flybase-updates@morgan.harvard.edu, FlyBase-error@ogre.lbl.gov *F Cc: laura.torroja@uam.es *F Dear Laura, *F Thank you for your error reports. In regard to this case: *F >CG18853 appears to be a hybrid of phr and CG12822 *F I think you are absolutely correct, but I am doubtful that it is *F an assembly error. We have seen a handful of cases like this, *F in regions where there is a tandem duplication. The duplication *F breakpoints are in the middle of two genes (in this case, phr *F and CG12822), producing a chimeric gene at the boundary. Often, *F there are intact duplicated genes between the breakpoints. You *F will note that CG30382 is an intact duplication of Prosalpha6. *F The original (unduplicated) order must have been: *F phr (+ strand), Prosalpha6 (- strand), CG12822 (+ strand) *F We will add an annotation comment to CG18853, describing *F it as chimeric gene and noting that it may not be functional. *F Thanks again for your input. *F Lynn Crosby *F FlyBase # *U FBrf0173221 *a Roth *b G.E. *t 2003.10.30 *T personal communication to FlyBase *u FlyBase error report for CG3522 on Thu Oct 30 05:35:19 2003. *F From: FlyBase-error@ogre.lbl.gov *F Date: Thu, 30 Oct 2003 05:35:19 \-0800 *F To: flybase-updates@morgan.harvard.edu *F Cc: groth@genetik.biologie.fu-berlin.de *F Subject: FlyBase error report for CG3522 on Thu Oct 30 05:35:19 2003 *F Error report from Guenther E. Roth (groth@genetik.fu-berlin.de) *F Gene or accession: CG3522 *F Release: 3 *F cDNA or EST error *F Gene cDNA sequence: *F >RE28156 complete sequence *F TCTTGCATTCGGTGTTCCTTTTGTTTCGATAGTGCAATTAATATAAATTGGGACAACTGC *F AAATCTAATAGTATTATTAGAACCGGAATTAGTTGTGCACCTTCTAGTATTAGAGACTTA *F TTAACATAACCAACCTGTGCCTACCGGAAATATAGAGGTACTTCTCGCAAATTATGCGGG *F TTCTTGCAAGATAATACGATACCATTTGTTTATGGCTGAGTAAAAAAAAAACAAAGAACT *F CCAGGCTGACGAGTCATTTGTGCGTATCGGCACTGAATCTGCTCCAGATTCGAAAACTAG *F GTTTATTTACATTTGCATGCAAAGACATGAGCTAGGCCAAATATAACCGCTCGCCATGTC *F CAATATGGATCCAAGTGATGTCCGCAGTACCGCCCAGCTCATCCTGGCCAACGCTCGGCA *F GGGAAATAGCGCTTACAACATGCAGTATGATATGTCACGGGCACATTCCATAAACCTGAT *F TACGGAGGACTTCTTGGCCGGTTATATGCAGGATGGCAGGATGTCCGTGGTTCGAAGGTT *F TTTCTGCCTCTTCGTCACATTCGACTTGGTCTTCGTTTCGCTGCTGTGGCTCATTTGCAT *F TGTGATCAATGGAGACAATATATTCACTGCCTTCCACAAACAAATCGTGGAGTACACCAT *F CTACAAATCGCTCTTTGATGTTGTGGCAGTCGCTATCTGCCGATTTTTGGTGCTCATATT *F TTTCTACGCCATATTGTACATCAATCACTGGTCCATCATAGCGCTCTCTACAAGTGGGTC *F TTGCTTGTTCCTCATCTCGAAGGTGTTTGTGTTCGATTGGCTGGATTCAAAGCAGCAGGT *F ATTTGAGGTAATCCTCATAATAACCTCGTTCATACTGGCTTGGGGAGAAGCCTGGTTCCT *F GGACTGTAGGGTGATTCCTCAAGAGCGACATGCCCAACACTATTTCCGGACTATGACTTC *F AAATGATCGCACACCCATGGAACAGCCTGCCATTTTGATTGAGCAAGAACGGCCTCCGCA *F AAGTGTAACCGATTTTTATTCACTCATGGACACGGCTCGTCATTCCGACGAGGAGGATGA *F GTTGGATGATGAGTACACACAAATGGGATTGGATTGCCTTCGAAAGGCCTACGAGATCAT *F CGAGTCAAGTGACTGGAAGGTGGAAAAAGTTAACCAGAAAGGCGACACCATACACAGCAC *F TCAGCGCGACAAGATTGGAAAGATCTACAAGTTGACGGCCCGCATCAAGTATCCTGCAAA *F GGCTCTGATGGAAGATCTGTTCTATCGCATTGAAGACTGTCCCAAGTGGAATCCTGCTCT *F TTTGGAGTCCAAGATAGTACGCAAAATCAACTCCTACACCGATATTACCTATCAGGTATC *F CGTGGGCGGAGGAGGTGGCATGGTGAAGAGCCGCGACTTCGTGAACTTGCGGTCTTGTAG *F GCTCTTTTACAATGGTCAAATCTGCGATGACGATGAGACGGCTCAGCTCAGCAGCGATGA *F TGGGAACAGCAGTCTAAATCGGTCTTGCGAGGGTAGTGTTAGTACCATTTCCGATGGTGA *F CTCAAACACCCCACTGCTGCCCAGTAGCGTGTCTAGTTGCAAGGCAACGTTTCCCACTTC *F ATCCAAGGGAGCCGCTATGCCTTTTGACACCCTGGGCAACAGCTTGGGCGCCAAGAGCCT *F AGGTCCCATCGTGAACTTTGACGAGGAGCCACCGCCATTGGATCAGGACGAGTTCGAGGA *F TGCTAAGGACAAGGTCGACGGCGAAGCGAATAACATGACGAAACCAAATGTACCCAGCGT *F GGGAAAAACCAAGGACAGGGTTTGGGTCACTTCGGCGGTTAGTGTGCAATATGCTGCCGT *F TCCACCTTCACCCAAATACACAAGAGGGCAGAACATTGTCAGTGGGTTTGCGTTTCGTGA *F AATCGTTGGAAAATCGGATAGCTGCATTGTGGAGTGGGTGCTTTGCCTAGACCTAAAAGG *F CTATATTCCTCGCTATGTCCTGGATGCAGCCCTCACCTCGTCCATGACCGACTACATTAG *F CAATCTGCGCAAGCATGTCAACGAACTGAGGCAGAAGGGGCGTGGTCGAGCCCCCAGGAC *F CCACTAGGAAATCAGTAATCCGTATTTTAATTTAAATCCCATTGTATCTCAAGCGAGTGC *F TGTTCGCAGCAAGGTATCTAATAGAATATTTCAATAATTTGAAGCAGGAGAGCGAGTTCA *F GTGCTGTAAGTTTATTGAGTATTTTATGTATATGTATGGCATAAGTTCTATGTACCTATA *F TGCACGGCTTACATTATAAATTTTATACACGAAAAAAAAAAAAAAAA *F Comments: We completely sequenced cDNA RE28156. *F cDNA LD23890 contains an obvious splice artefact: It contains 23 bp of Intron10. *F The translation of LD23890 therefore leads to a truncated peptide. *F Translation of RE28156 leads to a peptide identical with the peptide predicted *F by gadfly for CG3522. # *U FBrf0173223 *a Bejsovec *b A. *t 2004.2.9 *T personal communication to FlyBase *u *F To: bejsovec@duke.edu *F Subject: FlyBase query *F From: Gillian Millburn (Genetics) *F Date: Wed, 28 Jan 2004 19:15:32 \+0000 *F Hi Amy, *F I am curating your paper on eRF1 and eRF3 in Genetics: *F Chao et al., 2003, Genetics 165(2): 601--612 *F .. *F UAS-wgPE4 and UAS-wgPE13. I just wanted to check that these two *F constructs weren't originally made with an FRT cassette \- the reason I *F ask is that we have an HA tagged UAS-wgPE4 construct from: *F Hays et al., 1997, Development 124(19): 3727--3736 *F but that was made with an FRT cassette between the UAS and wg sequences *F to start with \- so I wanted to check that the UAS-wgPE4 construct *F described in your Genetics paper is a different construct. *F .. *F thanks, *F Gillian *F Date: Mon, 9 Feb 2004 11:28:32 \-0500 *F To: Gillian Millburn (Genetics) *F From: Amy Bejsovec *F Subject: Re: FlyBase query *F Hi Gillian, *F >Hi Amy, *F > *F >I am curating your paper on eRF1 and eRF3 in Genetics: *F > *F >Chao et al., 2003, Genetics 165(2): 601--612 *F > *F .. *F > *F >UAS-wgPE4 and UAS-wgPE13. I just wanted to check that these two *F >constructs weren't originally made with an FRT cassette \- the reason I *F >ask is that we have an HA tagged UAS-wgPE4 construct from: *F > *F >Hays et al., 1997, Development 124(19): 3727--3736 *F > *F >but that was made with an FRT cassette between the UAS and wg sequences *F >to start with \- so I wanted to check that the UAS-wgPE4 construct *F >described in your Genetics paper is a different construct. *F All of the wg constructs that we make have FRT sites flanking a polyA *F sequence, otherwise it is impossible to recover transformants. The *F UAS promoter suffers transient activation when the pUAST *F transformation vector is injected, and with something as potent as *F wingless downstream, any promoter leakiness will kill the embryo. *F The FRT cassette is flipped out in all the lines we use for our *F experiments: once a stably transformed line is recovered, it is *F crossed to the testis-specific beta-tubulin-promoter driven flippase. *F This flips out the cassette in every construct passed through the *F male germline (we recover individual lines and test each for *F transgene expression to make sure, but so far 100% of all our *F constructs have had the FRT cassette removed). *F This wgPE4 construct is different from the Hays et al. line because *F that line introduced the stop codon in a much more primitive way, by *F including the mutation in a PCR primer, and then recloning the PCR *F product back into the HA-tagged wild-type transgene. In the process, *F some of the sequences downstream of the stop were altered because of *F cloning issues. The new wgPE4 construct introduced the stop using a *F newer PCR-based methodology, and introduces the single nucleotide *F change with no other changes to the wingless sequence. The wgPE13 *F change was introduced in exactly the same way, so that these two *F transgenes could be compared directly. *F .. *F Amy Bejsovec, Ph.D. *F Duke University *F Dept. of Biology/DCMB Group *F Box 91000 *F B336 LSRC, Research Dr. *F Durham, NC 27708-1000 *F (919) 613-8162 office email: bejsovec@duke.edu *F (919) 613-8163 lab FAX: (919) 613-8177 *F (919) 613-8153 fly room # *U FBrf0173224 *a Cook *b K. *c I. *d Duncan *t 2003.10.9 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Oct 09 18:36:30 2003 *F To: rd120@gen.cam.ac.uk *F Subject: Note on Df(3L)v65c cytology *F Polytene squashes by Ian Duncan and myself show that Df(3L)v65c is *F associated with a heterochromatic inversion. Ian judged the breakpoint *F junctions to be 65D1-3/81 het and 64F2-5/het. Noncomplementation with *F Df(3L)v65c may be due to position effect variegation of wild type alleles *F on the deletion chromosome rather than deletion of genomic sequences. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173225 *a Toub *b O. *t 2003.10.22 *T personal communication to FlyBase *u *F From flybase-help@morgan.harvard.edu Wed Oct 22 20:53:04 2003 *F To: *F Subject: Question about a Transposon insertion site *F Dear Flybase, *F I have a Drosophila Melanogaster stock that has a P-element inserted in *F one of its genes. the P- element is: p{w+mC=lacW}l(3)s2214s2214 and *F the stock is purchased from Bloomington Drosophila Stock center. The *F cytological map in fly base locates this P-element to be at *F 2945944..2975369 on chromosome 3R(84c4-6 map location). However, if I *F blast the 79 bp flanking sequence of the P-element (given below) *F against the drosophila genome the homology is found at the CG10061 gene *F that maps to 2977094..2979905 on 3R(84c6 map location). The P-element *F is inserted at 2977691. I am a little confused could you please clarify *F this? *F Flanking sequence: *F gcgcagctgg tcgggattga tcttgaaccg ctgctttaga ccttcgccgc gacgcagaaaagggcgttta *F ggcttggccThank you in advance, *F Omid Toub. *F Date: Wed, 29 Oct 2003 16:08:04 \-0800 (PST) *F From: Sima Misra *F To: Omid Toub *F Cc: flybase-help@morgan.harvard.edu *F Subject: Re: Question about a Transposon insertion site *F I'm sorry for the confusion. I believe that no one has yet made the *F connection between this insertion, p{w+mC=lacW}l(3)s2214s2214, and the *F gene model, CG10061. So the location on the cytogenetic map may have been *F based purely on the in situ results, and not on the sequence information. *F Indeed, the BLAST results indicate that l(3)s2214 is in fact CG10061, and *F the insertion maps to 2977691 on 3R. *F Perhaps someone else at FlyBase has something more to add; if so, they *F will chime in. *F Hope this helps, *F Sima Misra *F FlyBase BDGP *F From sima@fruitfly.org Thu Oct 30 00:33:49 2003 *F To: Curators *F Subject: Re: Question about a Transposon insertion site (fwd) *F Camcur, Lynn, *F I think that this insertion indicates that l(3)s2214 and CG10061 should be *F merged. What do you think? The BLAST puts it firmly within an exon of *F CG10061. *F \-sima # *U FBrf0173226 *a Klaembt *b C. *t 2003.10.31 *T personal communication to FlyBase *u *F >From klaembt@uni-muenster.de Fri Aug 15 18:28:02 2003 *F To: m.ashburner@gen.cam.ac.uk *F From: Christian Klaembt *F Dear Michael, *F ... *F 4 years ago a gene was published FBrf0107610 named discs lost *F It turns out that the mutant has no discs, but it was mapped to the *F wrong transcription unit. It also has a different cellular phenotype. *F Last year a second gene nearby was named vanaso (which has a human *F homolog called codanin.1). When you read the paper the evidence for *F assigning the phenotype under study to this transcription unit is rather *F weak and since vanaso and alpha-spectrin share the first untranslated exon *F the phenotype could be equally well be linked to alpha- spectrin. *F We have now shown that the discs lost mutant corresponds to the gene called *F vanaso. *F What to do? *F We suggested in our paper that vanaso should be changed into discs lost. *F The transcription unit previously thought to be encoded by discs lost *F should be called according to its vertebrate homologue \- Patj. *F ... *F many thanks for your help *F best wishes christian *F \---------------------------------------------------------- *F Dr. Christian Klaembt *F Institut fuer Neurobiologie *F Universitat Munster *F Badestrasse 9 *F D-48149 Munster Germany *F Phone: xx49 251 83 2 1122 *F FAX: xx49 251 83 2 4686 *F email: klaembt@uni-muenster.de *F URL: http://www.uni-muenster.de/Biologie/Neurover/Neuro/welcome.html *F \---------------------------------------------------------- *F From klaembt@uni-muenster.de Fri Oct 31 15:06:29 2003 *F To: Rachel Drysdale (Genetics) *F Subject: Re: your dlt paper *F Dear Rachel the paper is now accepted, it should appear in the december *F issue of Dev Cell. *F best wishes christian # *U FBrf0173227 *a Roote *b J. *t 2003.11.4 *T personal communication to FlyBase *u *F From j.roote@gen.cam.ac.uk Fri Nov 28 11:05:19 2003 *F Subject: Re: Cat *F Date: Fri, 28 Nov 2003 11:05:56 \+0000 *F To: Rachel Drysdale (Genetics) *F Dear R, *F Df(3L)Cat (75C1;75F1) is reported to delete Ten-m (79E1-3). I believe *F this mistake must have arisen from Addison's paper (FBrf0082995) which *F states that Cat is lethal with l(3)00844. 00844 is an allele of Ten-m *F and is inserted in 79E1-3. *F 00844 was probably a typo for l(3)00864, which is inserted in 75E1-2. *F This interpretation is supported by the location of 'l(3)00844' *F implied in Fig 6A of FBrf0082995. *F J # *U FBrf0173228 *a Bloomington Drosophila Stock Center *b ?. *t 2003.12.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 18:40:21 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(3R)BSC42 *F Isolation and Characterization of Df(3R)BSC42 *F Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC42 was isolated as a P transposase-induced male recombination *F event involving P{PZ}l(3)rL203rL203 and P{EP}EP3088. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{PZ}l(3)rL203rL203/P{EP}EP3088 ca1 males. Polytene chromosome squashes *F showed the breakpoints 98B1-2; 98B3-5. The proximal breakpoint of *F Df(3R)BSC42 lies within the 98B1,2 doublet, leaving a portion of the *F doublet intact in the deletion chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173229 *a Bloomington Drosophila Stock Center *b ?. *t 2003.12.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 18:50:17 2003 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and Characterization of Df(3R)BSC43 *F Isolation and Characterization of Df(3R)BSC43 *F Rachel Andrade, Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC43 was isolated as a P transposase-induced male recombination *F event involving P{PZ}CG3119503806 and P{lacW}Rab11j2D1. The deletion *F was isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{PZ}CG3119503806/P{lacW}Rab11j2D1 ca1 males. Polytene chromosome *F squashes showed the breakpoints 92F7-93A1;93B3-6. Df(3R)BSC43 failed to *F complement Mvlm13 and P{PZ}Atpalpha01453a. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173230 *a Bloomington Drosophila Stock Center *b ?. *t 2003.12.2 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 22:13:40 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2R)BSC44 *F Cc: medeal@bio.indiana.edu *F Isolation and characterization of Df(2R)BSC44 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC44 was isolated as a P transposase-induced male recombination *F event involving P{lacW}l(2)k04222bk04222b and P{EP}CG14478EP2283. The *F deletion was isolated as a cn+-bw+ recombinant chromosome from the *F cross cn1 bw1 females X cn1 *F P{EP}CG14478EP2283/P{lacW}l(2)k04222bk04222b bw1 sp1; TMS, Sb1 *F P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 54B1-2;54B7-10. Df(2R)BSC44 failed to complement cnkE-2083, *F cnkk16314 and mblk07103. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173231 *a Thor *b S. *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Dec 01 21:55:29 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-roe.SP} and P{UAS-rn.SP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Stefan Thor, Harvard Medical School (11/03). *F P{UAS-roe.SP}18, P{UAS-roe.SP}88 and P{UAS-rn.SP}1 are homozygous viable *F and fertile, second chromosome insertions. *F P{UAS-rn.SP}32 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173232 *a Mlodzik *b M. *t 2002.5 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 14:29:25 2003 *F Date: Tue, 02 Dec 2003 09:29:22 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{arm-lacZ.V}MM1 insertion *F The following information was provided to the Bloomington Stock Center by *F Marek Mlodzik, Mount Sinai School of Medicine (5/02). *F P{arm-lacZ.V}MM1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion distal to 18A isolated by Marek Mlodzik and colleagues. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173235 *a Weber *b U. *t 2002.5 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 15:18:06 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Tub-Sec61Beta.V}1 *F The following information was provided to the Bloomington Stock Center by *F Ursula Weber, Mount Sinai School of Medicine (5/02). *F P{Tub-Sec61Beta.V}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173236 *a Weber *b U. *c M. *d Mlodzik *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 15:55:25 2003 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: UAS-Rho1 insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Ursula Weber and Marek Mlodzik, Mount Sinai School of *F Medicine (11/03). *F P{UAS-Rho1.N19}1.3 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Rho1.N19}4.3 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Rho1.V14}5.1, P{UAS-Rho1.V14.F39V}13 and P{UAS-Rho1.V14.E40L}9 are *F second chromosome insertions. *F P{UAS-Rho1.N19}2.1, P{UAS-Rho1.V14}2.1 , P{UAS-Rho1.V14.E40L}4 and *F P{UAS-Rho1.Sph}2.1 are homozygous viable and fertile, third chromosome *F insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173237 *a Dura *b J.M. *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 16:32:41 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: UAS-ttk insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jean-Maurice Dura, Institut de Genetique Humaine, CNRS *F Montpellier (11/03). *F P{UAS-ttk.p88}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-ttk.p69}2 is a homozygous viable and fertile, second chromosome *F insertion. *F ________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173238 *a Dura *b J.M. *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 16:48:53 2003 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GawB}7B *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jean-Maurice Dura, Institut de Genetique Humaine, CNRS *F Montpellier (11/03). *F P{GawB}7B is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173239 *a Szabad *b J. *c A. *d Szent-Gyorgyi *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 19:11:00 2003 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Lam.GFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Janos Szabad, Albert Szent-Gyorgyi Medical University (11/03). *F P{UAS-Lam.GFP}3-3 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Lam.GFP}3-2 is a second chromosome insertion. *F P{UAS-Lam.GFP}2-1 is a homozygous viable and fertile, third chromosome *F insertion. *F P{UAS-Lam.GFP}1-9 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion which is expressed more weakly than the other *F P{UAS-Lam.GFP} insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173240 *a Grieder *b N. *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 19:29:55 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UASp-GFPS65C-alphaTub84B}14-6-II *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Nicole Grieder, University of Basel (11/03). *F P{UASp-GFPS65C-alphaTub84B}14-6-II is a homozygous viable and fertile, *F second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173241 *a Green *b M. *t 2003.10 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 20:42:31 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Df(1)y-91g23 and yMG *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mel Green, University of California at Davis (10/03). *F Df(1)y-91g23 is a deletion of the X tip to 1B4-9. It deletes y and sc. *F yMG is a spontaneous mutation that arose on Dp(1;Y)y+sc. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173242 *a Weber *b U. *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 04 19:09:25 2003 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Cct16919 and psqF112 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Ursula Weber, Mount Sinai School of Medicine (11/03). *F P{lacW}Cct116919 is a P-element insertion generated in the screen *F described in Erdelyi et al. 1995 Nature 377(6549):524--527 (FBrf0083893). *F P{lacZ}psqF112, described in FBal0050311, is a P{PZ} insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173243 *a Deal-Herr *b M. *c K. *d Cook *t 2003.12.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 11 21:01:07 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(2R)BSC45 *F Isolation and Characterization of Df(2R)BSC45 *F Megan Deal-Herr and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2R)BSC45 was isolated as a P transposase-induced male recombination *F event involving P{SUPor-P}CG6424KG00595 and P{lacW}l(2)k07406k07406. *F The deletion was isolated as a cn+-bw+ recombinant chromosome from the *F cross cn1 bw1 females X cn1 *F P{SUPor-P}CG6424KG00595/P{lacW}l(2)k07406k07406 bw1 sp1; TMS, Sb1 *F P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 54C8-D1;54E2-7. Df(2R)BSC45 failed to complement rhi02086, *F POSHk15815 and Df(2R)14H10Y-53. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173244 *a Andrade *b R. *c K. *d Cook *t 2003.12.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Thu Dec 11 21:10:22 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(3L)BSC46 *F Cc: Rachel Andrade *F Isolation and Characterization of Df(3L)BSC46 *F Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3L)BSC46 was isolated as a P transposase-induced male recombination *F event involving P{PZ}fry02240 and P{EP}EP771. The deletion was isolated *F as a rhove-1-e1 recombinant chromosome from the cross rhove-1 pp *F e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 *F P{PZ}fry02240/P{EP}EP0771 e1 males. Polytene chromosome squashes showed *F the breakpoints 67C2-4;67C8-10. Df(3L)BSC46 failed to complement *F P{PZ}l(3)0185901859 and l(3)67BDp1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173245 *a Thor *b S. *t 2003.11 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Dec 02 13:49:32 2003 *F To: rd120@gen.cam.ac.uk *F Subject: rnGAL4-DeltaS *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Stefan Thor, Harvard Medical School (11/03). *F rnGAL4-DeltaS is a P transposase-induced derivative of *F P{GawB}rnGAL4-5. It is a presumed imprecise excision which retains GAL4 *F expression, but not miniwhite expression. It shows a rn null phenotype. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173246 *a Munoz *b E. *t 2003.12.10 *T personal communication to FlyBase *u *F From ermunoz@cnea.gov.ar Wed Dec 10 17:25:53 2003 *F To: flybase-updates@morgan.harvard.edu *F Subject: Drosophila data *F Dear colleges, *F The Drosophila lab. at the Argentine Atomic *F Energy Commission is being closed by the end of *F this year. Owing to this I'm in touch with Dr. Kevin *F Cook to whom I'm sending some neutron induced *F lethals and deletions from the base of the X chromosome *F covered by Dp (1;Y) mal+. He will surely get in touch *F with you in order to incorporate this information to *F the Flybase. The lethals can be divided in four *F groups: A, B, C and D. *F GROUP A: lethals i) described in the Flybase or *F mentioned as alleles or synonyms; ii) no stocks *F were available in Bloomington. *F GROUP B: lethals that as far as I know do not appear *F in the Flybase. Since I think information on their *F localization may be of use to those interested in the *F proximal end of the X, the mapping details are *F provided in the attached file. *F GROUP C: lethals, i) mentioned in the Flybase; *F ii) with stocks available in Bloomington. *F GROUP D: Although fully analyzed, I haven't *F published the location of these lethals and deficiencies *F (about 20) and I believe they do not appear in the *F Flybase. Unfortunately we lost the stocks, and after *F giving copies away we also lost their track. Actually, *F I don't know if somebody described them or *F still have a copy of the stocks. With this in mind I *F thought it may be of interest to incorporate the *F information on their mapping in the Flybase. *F Therefore, if you believe my data on the localization *F of the lethals could be useful, please let me know *F and I'll be glad to send it. *F Please find attached: 1) A letter sent to Dr. Cook; *F 2) Mapping of GROUP B lethals. *F Best regards, *F Dr. Enzo R. Munoz *F Radiobiologia *F Comision Nacional de *F Energia Atomica *F Av. Gral. Paz 1499 *F 1650 \- San MNartin (Buenos Aires) *F Argentina *F \-------------------------- *F GROUP B *F Stocks of lethals covered by Dp(1;Y)mal+ that do not appear in the Flybase. *F bbl: bb lethal of unknown origin. \-(Fb): The chromosome appears in the Flybase *F Lethals and deficiencies whose designation does not start with prefixes 14 to *F 19 are from Lifschytz and Falk collection. Their location and subsequent *F corrections can be found in the following references (by no means exhaustive): *F Lifschytz, E. and Falk, R.: Mutation Res. 6, 235-244 (l968); Mutation Res. 8, *F (1969) 147-155; DIS 45 (1970), 44. Falk, R., Mutation Res. 20, (1973) *F 287-290. Lifschytz, E. Mutation Res. 13 (1971) 35-47. Schalet, A. and *F Lefevre, G., Chromosoma 44 (1973) 183-202; The Genetics and Biology of *F Drosophila, Vol 1b (1976) 847-902 *F 10.-Df(1)16-1-80 *F Not covered by Dp(1;Y)BSYy+. Lethal with Dfs.B-111 and 17-196 and with *F lethals A-200, Q-56, Q-456. Viable with Dfs. B-12, B-57, 16-3-35 (Fb), 17-25 *F (Fb) and n23 (Fb) and with lethals E-54, 14-1, x-3, 114, P-19,Q-463, X-4, *F R-9-18, 3-Des. Normal with bbl. *F bk1: Between eo and wap. Left limit from non-inclusion of l(1)E-54 (eo); *F right, from inclusion of A-200 (wap). *F bk2: Between uncl and l(1)114 (fog). Left limit from inclusion of uncl; right, *F from non-inclusion of l(1)114. *F Deficiency with similar break points limits: 16-2-13 (Fb) *F 11.-Df(1)16-3-20 *F Not covered by Dp(1;Y)BSYy+. Lethal with Df(1)B-111 and with lethals *F A-200, Q-56, 114, X-3, P-19, Q-463, X-4, R-9-18, 3-Des, and bbl. Semi-lethal *F with Q-456 (uncl). Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb) and *F 17-322 and l(1)E-54. *F bk1: Between eo and wap. Left limit from non-inclusion of l(1)E-54 (eo); right *F from inclusion of A-200 (wap). *F bk2: To the right of bb ?, is lethal with bbl. *F Deficiencies with similar break points limits: 16-2-14, 15-13, 15-17, 18-67 *F 12.-Df(1)15-13 *F See above Df(1)16-3-20 *F 13.-Df(1)16-3-34 *F Not covered by Dp(1;Y)BSYy+. Letal with Df(1)B-111 and with lethals A-200 *F and Q-56. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), 17-196 and *F with lethals E-54, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, 14-1.- *F Normal with bbl. *F bk 1: Between eo and wap. Left limit from non-inclusion of l(1)E54, l(1)14-1 *F (eo); right from inclusion of l(1)A-200 (wap). *F bk 2: Between l(1)Q-56 and uncl. Left limit from inclusion of l(1)Q56; right *F from non-inclusion of Df(1)17-196 and l(1)Q-456 (uncl). *F Deficiency with similar break points limits: Df(1)17-326 *F 14.-Df(1)16-3-93 *F Not covered by Dp(1;Y)BSYy+. Lethal with Df(1)B-111 and with lethals *F A-200, Q-56, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des. Semi-lethal with *F l(1)Q-456 (uncl). Viable with Dfs.B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), 17-322 *F and l(1)E-54. Normal with bbl. *F bk 1: Between eo and wap. Left limit from non-inclusion of l(1)E-54 (eo) and *F Df(l)B-12; right from inclusion of A-200 (wap). *F bk 2: Between su(f) and bb. Left limit from inclusion of 3-Des (su(f)); right *F from non-inclusion of bb. *F Dfs. with similar break points: 16-2-17, 16-3-71, 16-3-145, 18-6, 17-319, *F 18-57, 18-30 (male sterile), 17-459 (male sterile). *F l5.-Df(1)18-6 *F See above Df(1)16-3-93 *F 16.-Df(1)16-3-211 *F Not covered by Dp(1;Y)BSYy+. Lethal with Df(1)B-111 and with lethals *F A-200, Q-56, 114, x-3, P-19, Q-463, x-4, R-9-18, and 3-Des. Semi-lethal with *F l(1)Q-456 (uncl). Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), *F 17-322 and with l(1)E-54 (eo). It shows bb with bbl *F . *F bk1: Between eo and wap. Left limit from non-inclusion of l(1)E54 (eo) and *F Df(1)B-12; right from inclusion of l(1)A-200. *F bk2: Disrupt bb. *F 17.-Df(1)14-11 *F Not covered by Dp(1;Y)BSYy+. Lethal with Dfs. B-111, B-57, B-12, 16-3-35 *F (Fb), T2-14-A, and with lethals A-200, Q-56, Q-456, B-56, B-214, W-2, W-4, *F LB20, E-81, AA-33, 17-62 (Fb), 14-1, 16-3-212 (Fb). Viable with Dfs. 17-25 *F (Fb), 18-61, mal-10 and with lethals 114, x-3, P-19, Q-463, x-4, R-9-18, *F 3-Des. Normal with ot and bbl. *F bk1: Between mell and l(1)AA-33 (leg, run). Left limit from non-inclusion of *F Dfs. mal-10 and 17-25 (Fb), does not show mell; right from inclusion of AA-33. *F bk 2: Between uncl and l(1)114 (fog). Left limit from inclusion of Q-456 *F (uncl); right, from non-inclusion of l(1)114 *F Df. With similar break points limits: 17-241 *F 18.-Df(1)17-241 *F See above Df(1)14-11 *F 19.-Df(1)17-196 *F Not covered by Dp(1;Y)BSYy+. Lethal with Df(1)B-111 and with lethals 114, *F x-3, P-19, Q-463, x-4, R-9-18, 3-Des. Semi-lethal with Q-456. Viable with Dfs. *F B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), and lethals A-200, Q-56 (intro). Normal *F with bbl. *F bk1: Between l(1)Q-56 and uncl. Left limit from non-inclusion of l(1)Q-56; *F right from inclusion of Q-456 (uncl) *F bk2: Between su(f) and bb. Left limit from inclusion of 3-Des; right, normal *F with bbl *F Df. with similar break points limits: 17-408 (Fb) *F 20.-l(1)16-3-190 *F Not covered by the Dp(1;Y)BSYy+. Lethal with Df(1)B-111 and with lethal *F 114. Viable with Dfs. B-57, B-12, 16-3-3 (Fb), 17-25 (Fb), 17-123 (Fb), *F 16-1-80, 16-2-13 (Fb) and with lethals A-200, Q-56, Q-456, x-3, P-19, Q-463, *F x-4, R-9-18, 3-Des. Normal with bbl. *F Location: allele of l(1)114 (fog). l(1)16-3-190/Dp(1;Y)mal+ males are *F sterile. *F 21.-l(1)16-2-27 *F Not covered by Dp(1;Y)BSYy+. Lethal with Dfs. B-12, 17-137 (Fb) and with *F l(1)E-54. Viable with Dfs. B-111, B-57, 16-3-35 (Fb), 17-25 (Fb), 16-2-14, *F 17-351 (Fb) and with lethals A-112, LB 20, w-4, w-2, B-214, B-56, w-1, A-118, *F R-9-28, E-81, AA-33.Shows eo with 17-36 (Fb). *F Location: allele of l(1)E-54 (eo), isolated as lethal owing to the poor *F viability of males. *F Alleles: 16-3-119, 17-427, 17-260 (Fb), 17-36 (Fb), 14-1 *F 22.-Df(1)19-18 *F Lethal with Dfs. B-111, B-57, B-12 and 16-3-35 (Fb) and with lethals A-200, *F Q-56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, *F LB20, 17-62, 14-1, 16-3-212, A-118, R-9-28, E-81, AA-33. Viable with Dfs. *F 17-25 and mal-10. Normal with ot. Lethal with bbl. Males *F Df(1)19-18/Dp(1;Y)mal+ are sterile. *F bk1: Between mell and leg. Left limit from non-inclusion of mell, viable with *F Df(1)17-25; right from inclusion of l(1)AA-33 *F bk2: To the right of bb?, is lethal with bbl *F Df. with similar break points: Df(1)16-3-129.- Df(1)16-3-129/Dp(1;Y)mal+ *F males are fertile. # *U FBrf0173247 *a Cavener *b D. *t 2003.12.17 *T personal communication to FlyBase *u *F From flybase-help@morgan.harvard.edu Wed Dec 17 02:35:11 2003 *F To: flybase-help@morgan.harvard.edu *F Subject: FlyBase-NG Help Mail *F comments: PEK and PERK is the same gene (i.e. they are synonyms for the same *F gene more formally known as EIF2AK3. I originally sent the map location of *F this gene and called it PERK, the term that is used most extensively in the *F literature. Apparent the annotators of the Drosophila genome discovered the *F coding sequence of this gene but failed to note that I had already given the *F approximate map location to flybase and instead gave it the name PEK, There *F are no two independent entries in flybase, one for PEK and one for PERK *F without any cross reference to each other. *F mailto: flybase-help@morgan.harvard.edu *F realname: Doug Cavener *F reply-to: drc9@psu.edu *F Sent from computer 68.168.175.108 # *U FBrf0173248 *a Luschnig *b S. *t 2003.12.17 *T personal communication to FlyBase *u *F From luschnig@pmgm2.stanford.edu Thu Dec 18 03:58:01 2003 *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Thu, 18 Dec 2003 03:58:01 \+0000 *F X-Sender: luschnig@cmgm.stanford.edu (Unverified) *F X-Mailer: QUALCOMM Windows Eudora Version 5.2.0.9 *F Date: Wed, 17 Dec 2003 19:57:02 \-0800 *F To: flybase-updates@morgan.harvard.edu *F From: Stefan Luschnig *F Subject: Re: existence-uncertain genes *F Mime-Version: 1.0 *F Hi, *F I had previously exchanged emails with Sima Misra about the genes annotated *F in FlyBase as 'existence-uncertain'. Initially, I had found a few of these *F genes to show high fold-changes in an expression profiling experiment *F comparing wildtype and rib mutant embryos. Out of curiosity I went on and *F retrieved all the CGs (321) annotated in Flybase as 'existence-uncertain', *F and looked them up in a series of array experiments done in our lab. *F Interestingly, many of these genes show marked changes in expression levels *F between these different experiments, suggesting they may in fact be 'real' *F genes (e.g., some seem to be specifically expressed in the male germline, *F others in the third instar larval gut). *F Sima had suggested at some point that I send these data, which I am doing *F here; I attached a pdf file with clustered expression profiles for these *F genes, as well as an Excel file with the data. *F I hope this might be helpful in re-annotating these genes. The labelling of *F the arrays should be somewhat self-explanatory, but I'll be happy to *F answer any questions, of course. *F Stefan Luschnig *F File deposited: Luschnig.2003.12.17-1.pdf ; File date: 2003.12.17 ; File size: *F 1106490 ; File format: pdf *F File deposited: Luschnig.2003.12.17-2.xls ; File date: 2003.12.17 ; File size: *F 225792 ; File format: xls # *U FBrf0173249 *a Matthews *b K. *t 2003.12.17 *T personal communication to FlyBase *u *F From matthewk@indiana.edu Wed Dec 17 20:43:56 2003 *F Subject: assorted Aberrations comments *F To: flybase-updates@morgan.harvard.edu *F Cc: matthewk@fly.bio.indiana.edu *F These are notes on things I came across in FlyBase Aberration records as I *F worked on the new Class assignments. *F FBab0027958 Df(2L)sna2.4LGYLR *F Df is sna- cact-, dup is 33B1;35C1-2, therefore presumably deficient *F for at least 35D2;35F9-11 *F FBab0024736 T(2;3)sna2.40 *F This is a transposition, so should be Tp(2;3)sna2.40. The deficiency seg *F is sna- and the duplication seg is sna+ \- ref is DIS 66 (1987), p. 259, *F where the genotype of stock D710, Df(2L)sna2.40L Dp(2;2)GYLR/CyO; *F Dp(2;3)sna+2.40/TM3, Sb, makes it clear that the parental T(2;3)sna2.40 *F must be a Tp (and that the transposed segment carries sna). *F FBab0024625 *F Progenitors: In(1)scS1 and In(1)EN *F Mutagen: recombination *F should be duplicated for 1A;1B3 \+ 20C;h32 *F FBab0027225 *F Progenitors: In(1)scV2 and In(1)sc4 *F Mutagen: recombination *F FBab0024626 *F Progenitors: In(1)y3P and In(1)scV2 *F Mutagen: recombination *F FBab0024631 *F Progenitors: In(2L)t and In(2L)Cy *F Mutagen: recombination *F should be duplicated for 22D1-2;22D3-6 and 33F5-34A1;34A8-9 *F FBab0023861 *F Progenitors: In(3L)C90 and In(3L)P *F Mutagen: recombination *F deficient for 62B8-10;63B8-11 *F duplicated for 72E1-2;80C-F *F FBab0023862 *F Progenitors: In(3L)C90 and In(3L)P *F Mutagen: recombination *F deficient for 72E1-2;80C-F *F duplicated for 62B8-10;63B8-11 *F FBab0023871 *F Progenitors: In(3LR)79i and In(3LR)bxd194 *F Mutagen: recombination *F duplicated for 67B11-C1;80 and 87D5-13;89E1-2 *F FBab0023878 *F Progenitors: In(3LR)79i and In(3LR)bxd194 *F Mutagen: recombination *F deficient for 67B11-C1;80 and 87D5-13;89E1-2 *F FBab0023879 *F Progenitors: In(3LR)bxd194 and In(3LR)C190 *F Mutagen: recombination *F duplicated for 80;69F3-4 and 89D;89E1-2 *F FBab0023876 *F Progenitors: In(3LR)P91 and In(3LR)C190 *F Mutagen: recombination *F deficient for 67C10-D1;69F3-4 \+ 81F;89D *F FBab0023883 *F Progenitors: In(3R)221 and In(3R)P *F Mutagen: recombination *F deficient for 82F;89C-D *F FBab0023891 *F Progenitors: In(3R)221 and In(3R)P *F Mutagen: recombination *F duplicated for 82F;89C-D # *U FBrf0173250 *a Munoz *b E. *t 2004.1.5 *T personal communication to FlyBase *u *F From ermunoz@cnea.gov.ar Mon Jan 05 12:53:59 2004 *F To: rd120@gen.cam.ac.uk *F Subject: Drosophila... *F FLYBASE *F (Suggestions, amendments, additions...) *F Df (1) 18-80 *F I mapped this neutron induced small (probably a two complementation units) *F deletion as being allelic to l (1) w-2 (fliI14) and l (1) w-4 (l (1) 19Fe4). *F In the Flybase Report of gene fliI it can be seen that under synonyms appears l *F (1) l8-80. The designation 18-80 also appears as a synonym of fliI18-80 . Since *F years ago I sent a bunch of stocks to Miklos (though I don¹t remember if this *F particular one was included) I wonder if we are not talking about the same *F chromosome . My data indicates that Df (1) 18-80 is: *F Lethal with Df (1) B-12 and with lethals w-2 and w-4. Viable with Dfs. B- *F 111, B-57, 16-3-35 (Fb), 17-25 (Fb), 17-137 (Fb), 16-2-14 and A-118 and *F with lethals E-54, A-112, LB20, B-214, B-56, w-1, R-9-28, E-81 and AA-33. *F Normal with ot and with bbl. *F bk1: Between l ( 1) B-214 and w-2. Left limit from non-inclusion of B-214; *F right from inclusion of w-2. *F bk2: Between l (1) w-4 and l (1) LB20. Left limit from inclusion of w-4; right *F from non-inclusion of LB20. *F l (1) l7-169 *F Is a male sterile double lethal. One is allelic to vao (vao2); the other is *F allelic *F to run (run19). Thus, run has 3 neutron induced alleles (all are male sterile): *F 17-169 (run19), 17-26 (run18) and 17-44 (to be added to the Flybase). *F In vao2, run18 and run19 please correct the spelling: is not Munos, but Munoz. *F eo *F l (1) 17-36 (eo33) (Fb), l (1) 17-260 (eo34) (Fb), and lethals: 14-1, 17-427, *F 16- *F 3-119 and 16-2-27 are all neutron induced alleles described under l (1) 16-2- *F 27 in the list of group B lethals. *F In Flybase l (1) 17-360 (eo17-360) appears as synonym of l (1) 17-260 (eo34) but *F it is actually a different lethal. *F 17-360 is a male fertile allele of shak B (shak B20) (Fb), l (1) 17-96 (shak18) *F (Fb) and l (1) 17-189 (shak19) (Fb); the two latter are male sterile. In shak *F B18, *F B19 and B20 please correct the spelling: is not Munos but Munoz. *F Df (1) 16-2-19 *F Polytene analysis by Schalet and Lefevre (see reference in Group B Lethals). *F l (1) 16-3-212 (unc2) (Fb) *F This is the original mutant used to describe the 'new?' unc because the *F phenotype was similar to a lost mutant described by Famy (see Schalet and *F Lefevre). In Flybase under synonyms of the gene unc appears l (1) 6-3-212. *F I¹m sure l (1) 16-3-212 and l (1) 6-3-212 refers to the same lethal. *F l (1) 16-2-9 *F Is a double lethal. One is allelic to l (1) x-4 (sph) and the other is between *F wap *F and uncl and lethal with l (1) Q-56.- Males carrying Dp (1;Y) y+ mal106 are *F sterile. *F Df (1) sp (Jlc) *F In the Flybase under synonyms appear: l (1) 17-18 and Df (1) 17-8. *F I didn¹t finished the analysis of my neutron induced l (1) l7-18. I lost my copy *F and lost control over the stocks left in Leiden. *F I think that Df (1) l7-8 refers to the spontaneous lethal described by Schalet *F in *F Mutation Research 163, 115-144. *F I recovered a neutron induced Df (1) 17-8. It is a large deficiency that maps in *F the proximal end of the X. Although I don¹t know if it has been used by others *F I think it may be wise to name it as Df (1) n17-8 (n for neutron). *F Df (1) n 17-8 : Lethal with Dfs B-111 and B-12 and with lethals A-200, Q- *F 56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, *F LB20, 17-62 (Fb) bbl and 14-1. Viable with Dfs. B-57, 16-3-35 (Fb) and 17- *F 25 (Fb) and with lethal 16-3-212 (Fb). Normal with ot. *F bk1: Betweem unc and lfl. Left limit from non inclusion of Df (1) B-57 and l *F (1) 16-3-212; right from inclusion of l (1) B-56. *F bk2: To the right of bb? from lethality with bbl. *F Hlc17-62 *F It may be convenient to mention that l (1) 17-62 is neutron induced. I isolated *F and localized it as an allele of l (1) A112. # *U FBrf0173251 *a Misra *b S. *t 2004.1.7 *T personal communication to FlyBase *u *F From sima@fruitfly.org Wed Jan 07 19:08:59 2004 *F To: Rachel Drysdale , *F Madeline Crosby *F Cc: Andy Schroeder , , *F *F Subject: Re: LysE or LysC *F There was a typo in our Genome Biology paper, FBrf0155827, in this line: *F 'Finally, in order to allow the construction of a wild-type proteome from *F the mutant sequenced y1; cn1bw1sp1 strain, we replaced annotated sequences *F from known mutated genes (y, cn, bw, MstProx, LysE, Rh6) with RefSeq *F wild-type sequences from GenBank with an appropriate note.' *F The LysE gene is not mutated in this strain; rather, the mutated gene is *F LysC. We apologize for any confusion may have this caused. *F Sima Misra *F FlyBase BDGP # *U FBrf0173252 *a Munoz *b E. *t 2004.1.5 *T personal communication to FlyBase *u *F From ermunoz@cnea.gov.ar Mon Jan 05 12:59:56 2004 *F To: rd120@gen.cam.ac.uk *F Subject: Drosophila II... *F Dear Rachel, *F Please find attached the file with information *F on Group D lethals. *F Best regards. Enzo *F \-------------------------------------------------------------------------- *F GROUP D LETHALS *F Though not stated in each case, all lethals are covered by the Dp (1;Y) mal+. *F (Fb): Flybase *F \-Df(1) n20 (Fb), (Syn. 17-274) *F \-Df(1)17-148 (Fb) and *F \-Df (1)17-441 *F have all similar break points limits. The three are lethal with Df(1)n23 (Fb), *F (Syn. *F Df(1)17-123) and viable with Df(1)n49 (Syn. Df(1)17-439 (Fb)). *F The data on the Flybase under _______n49____ *F 'Genetic data about other abe.' *F (17-439 and n20) needs to be *F corrected according to this scheme ____n23_____ *F __ n20, 17-148, *F 17-441_ *F Break points in Fb: O.K. Additional information: *F Lethal with Dfs. B-111 and Df(1)17-19 and with lethals Q-463, x-4, R-9-18, *F 3-Des. *F Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb) and 17-252 (Fb) and with *F lethals A-200, Q-56, Q-456, 114, x-3, P-19, Q-463. Normal with bbl. *F Df(1)18-76 *F Lethal with Df(1) B-111 and with lethals A-200, Q-56, Q-456 and 114. Viable *F with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), n23 (Fb) and with lethals x-3, *F P- *F 19, Q-463, x-4, R-8-18, 3-Des, E-54 and 14-1. Df(1)18-76/Df(1)n23 or /Df(1)18- *F 61 or /Df(1)16-1-79 or /Df (1)16-2-3, shows visible (abnormal wings, veins *F and *F bristles, ret ?). Normal with bbl *F bk 1: Between eo and wap. Left limit from non-inclusion of l (1)E-54; right from *F inclusion of A-200 *F bk 2: Between l(1)114 (fog) and l(1)x-3 (stnA). Left limit from inclusion of *F ll4 (and *F ret ?); right from non-inclusion of x-3. *F _________18-76_________ret ? *F ......._____n23____ *F ......._____18-61_____________ *F ......._____16-1-79_______ *F ......._____16-2-3________ *F ___________________________________________________________________ *F Df(1)18-61 *F Not covered by Dp (1;Y) BS Y y+. Lethal with Df (1)B-111 and with lethals *F x-3, P- *F 19, Q-463, x-4, R-9-18, 3-Des, bbl. Viable with lethals A-200, Q-56, Q-456, 114 *F and 16-3-190 and with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb). With Dfs, 18-76 *F and 17-454 shows visible (see 18-76) *F bk1: Between l (1)114 and l (1)x3. Left limit from non-inclusion of 114; *F right from *F inclusion of x-3 (and ret ? ). *F bk 2: To the right of bb *F ___________________________________________________________________ *F Df(1)16-1-79 and 16-2-3 *F Both are male sterile. Lethal with Df(1)B-111 and with lethals x-3, P-19, *F Q-463, x- *F 4, R-9-18, 3-Des. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), l7-466 *F (Fb) and 18-76 (it shows visible, see l8-76), and with lethals A-200, Q-56, *F Q-456, *F 114. Df(1)16-1-79 has normal phenotype with bbl; Df(1)16-2-3 was not tested with *F bbl *F bk1: Between l(1)114 and l (1)x3. Left limit from non-inclusion of 114; right *F from *F inclusion of x-3 (and ret ?) *F bk2: Df(1)16-1-79 : Between l(1)3-Des (su(f)) and bb. Left limit from *F inclusion of *F 3-Des; right from non inclusion of bb. *F Df(1)16-2-3: To the right of su(f) from inclusion of 3-Des . *F ___________________________________________________________________ *F Df(1)18-74 *F Covered by Dp(1;Y)BS Y y+ . Male sterile. Lethal with Df (1)B-111 and with *F lethals Q-463, x-4. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), l7-25 (Fb), *F 16-185 *F (Fb), 17-252 (Fb) and with lethals A-200, Q-56, Q-456, 114, x-3, P-19, R-9-18, *F 3- *F Des. Normal with bbl. *F bk1: Between l(1)P-19 (20 Ca) and Q-463 (20 Cb). Left limit from non-inclusion *F of *F P-19; right from inclusion of Q-463. *F bk2: Between x-4 (sph) and l (1)R-9-18. Left limit from inclusion of x-4; *F right from *F non-inclusion of R-9-18. *F _________________________________________________________________Df *F (1)17-454 *F Not covered by Dp (1;Y) BS Y y+. Male sterile. Lethal with Df(1)B-111 and with *F l(1)114. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), l7-25 (Fb), 16-1-80 and *F n23 *F (it shows visible, ret ? see Df(1)18-76) and with lethals A-200, Q-56, *F Q-456, x-3, *F P-19, Q-463, x-4, R-9-18, 3-Des and l6-1-49. Normal with bbl *F bk1: Between l(1)Q-456 (uncl) and l(1)114. Left limit from non-inclusion of Q- *F 456; right from inclusion of 114. *F bk2: Between 114 (or ret ?) and x-3 . Left limit from inclusion of ret ?; *F right from *F non-inclusion of x-3. *F __________________________________________________________________ *F Df(1)17-19 *F Not covered by Dp (1;Y)BS Y y+. Lethal with Df(1)B-111 and with lethals 114, x- *F 3, P-19, Q-463, 16-3-190. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), *F 16-1-80 and 17-87 and with lethals A-200, Q-56, Q-456, x-4, R-9-18 and 3-Des. *F Normal with bbl *F bk1: Between l(1)Q-456 (uncl) and l(1)114 (fog). Left limit from non-inclusion *F of *F Q-456; right from inclusion of 114. *F bk2: Between l(1)Q-463 (20Cb) and x-4 (sph). Left limit from inclusion of Q-463; *F right from non inclusion of x-4. *F __________________________________________________________________ *F Df (1)17-401 *F Not covered by Dp (1;Y)BS Y y+. Lethal with Df (1)B-111 and with lethals 114, x- *F 3, P-19, Q-463, x-4, R-9-18, 3-Des. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), *F 17- *F 25 (Fb) and 16-1-80 and with lethals A-200, Q-56 and Q-456. Normal with bbl. *F bk1: Between uncl and fog . Left limit from non-inclusion of l (1) Q-456; right *F from inclusion of l (1) 114. *F bk2: Between l (1)3-Des (su(f)) and bb. Left limit from inclusion of 3-Des; *F right *F from non-inclusion of bb. *F __________________________________________________________________ *F Df(1)17-228 and Df(1)17-369 *F Break point similar to Df (1) 17-137 already described in the Flybase. *F Additional *F information: Lethal with Dfs. B-111, B-12 and with lethals A-200, Q-56, Q-456, *F 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, E-54 and 14-1. Viable with Dfs. B-57, *F 16-3-35 (Fb), 17-25 (Fb), 16-1-29 (Fb) and 18-80 (Fb) and with lethals B-56, B- *F 214, w-2, w-4, LB20, 17-62 (Fb). Normal with ot and with bbl bk1: Between l *F (1)A-112 (Hlc) and l (1)E-54 (eo). Left limit from non-inclusion of A-112, right *F from inclusion of E-54. *F bk2: Between su(f) and bb. Left limit from inclusion of 3-Des, right from non- *F inclusion of bb. *F Df(1)17-412 *F Break points similar to Df(1)17-59 already described in the Flybase. The only *F difference is that Df (1)17-412/Dp (1;Y) mal+ males are sterile. *F Additional information: Lethal with Dfs. B-111 and B-12 and with lethalsA-200, *F Q-56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, LB20, 17-62 (Fb) and *F 14- *F 1. Viable with Dfs. B-57, 16-3-35, 17-25, 16-129 (Fb), 18-80 (Fb) and with *F lethals *F B-56, B-214, w-2, w-4, 16-3-212 (Fb). Normal with ot and with bbl. *F bk1: Between l (1)w-4 (l (1)19-Fe4) and LB20.Left limit from non-inclusion of w- *F 4, right from inclusion of LB20. *F bk2: Between su(f) and bb. Left limit from inclusion of l (1) 3-Des, right *F from non- *F inclusion of bb *F ___________________________________________________________________ *F Df(1)16-1-88 *F Lethal with Dfs. B-111, B-57, B-12, A-118 and 16-3-35 and with lethals A-200, Q- *F 56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, *F LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), , R-9-28, E-81 and bbl. Viable with Dfs. *F 17-25 (Fb) and mal-10 and with l (1)AA-33. Normal with ot. Df (1)16-1-88/Dp *F (1;Y) mal+ males are sterile. *F bk1: Between l (1)AA-33 (run, leg) and l(1) E-81. Left limit from *F non-inclusion of *F AA-33 and mal-10, right from inclusion of l (1) E-81. *F bk2: To the right of bb ?. Left limit from the inclusion of 3-Des; right from *F lethality *F with bbl. *F ___________________________________________________________________ *F Df (1)19-2 *F Break Points similar to Df (1) 17-489 already described in the Flybase. *F Additional *F information: Df (1)19-2/Dp(1;Y)mal+ males are sterile. Lethal with Dfs. B-111, *F B- *F 57, A-118, B-12 and T2-14A and with lethals A-200, Q-56, Q-456, 114, x-3, P-19, *F Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, LB20, l7-62 (Fb), 14-1, 16-3- *F 212 (Fb) and bbl. *F Viable with Dfs. 16-3-35 (Fb) and 17-25 (Fb) and with lethals R-9-28, E-81 and *F AA-33. *F Normal with ot. *F bk1: Fb *F bk 2: Fb *F Df (1) 19-18 and Df (1) 16-3-129 *F Lethal with Dfs. B-111, B-57, A-118, B-12 and 16-3-35 and with lethals A-200, Q- *F 56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, *F LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), , R-9-28, E-81, AA-33 and bbl. Viable *F with *F Dfs. 17-25 (Fb) and mal-10. Normal with ot. Df (1)19-18/Dp (1;Y) mal+ males are *F sterile. *F bk1: Between mell and l(1)AA-33. Left limit from viability with Df (1) 17-25; *F right *F from inclusion of AA-33. *F bk2: Left limit from inclusion of l (1)3-Des (su(f)); right to the right of bb *F ?, from *F lethality with bbl. *F ___________________________________________________________________ *F Df (1) 16-3-139 *F Lethal with Dfs. B-111, B-57, B-12, 16-3-35 and T2-14 A and with lethals A-200, *F Q-56, Q-456, 114, B-56, B-214, w-2, w-4, LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), *F E-81 and AA-33. Viable with Dfs. 17-25 (Fb), n20 (Fb) and mal-10 and with *F lethals x-3, P-19, Q-463, x-4, R-9-18, 3-Des. With Dfs. n24 and n23 (Fb) it *F shows *F visible, ret ? . Df (1)16-3-139/Dp (1;Y) mal+ males are sterile. *F bk1: Between mell and l (1)AA-33. Left limit from viability with Df (1) 17-25; *F right from inclusion of AA-33. *F bk2: Between ret ? and x-3 (stnA). Left limit from inclusion of ret; right *F from non- *F inclusion of x-3. *F Df (1)17-244 *F Lethal with Dfs. B-111, B-57 and B-12 and with lethals A-200, Q-56, Q-456, 114, *F x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, LB20, 17-62 (Fb), *F 14-1, 16-3-212 (Fb) Viable with Dfs. 16-3-35 (Fb), 17-25 (Fb) and A-118 and with *F l (1) 17-169 (Fb). The latter has two separate lethals, one is allelic to run *F (run19) *F and the other to vao . Normal with ot and with bbl *F bk1: Between vao and unc. Left limit from non-inclusion of l (1)17-169 and Df *F (1) *F A-118; right from inclusion of l(1) 16-3-212. *F bk2: Between su(f) and bb. Left limit from inclusion of 3-Des; right from non- *F inclusion of bb *F Df (1) 18-36 *F Lethal with Dfs. B-111, B-57, B-12, 16-3-35 (Fb), 17-25 Fb), A-118, T2-4A and *F 16-2-5 and with lethals A-200, Q-56, Q-456, 114, x-3, P-19, Q-463, x-4, *F R-9-18, 3- *F Des, B-56, B-214, w-2, w-4, LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), E-54, A-112, *F w-1, R-9-28, E-81, AA-33, 19Cc, 16-3-98 (Fb), 16-1-27 (Fb), 17-234 (Fb). Visible *F with ot, bbl and mal. *F bk1: To the left of ot, from inclusion of ot. *F bk2: disrupt bb *F ___________________________________________________________________ *F Df (1)17-322 *F Lethal with Dfs. B-12, B-57, 16-3-35 (Fb), 17-25 (Fb) and 17-137 (Fb) and with *F lethals E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, A-118, R-9-28, E-81 and *F AA-33. Viable with Dfs. B-111, 16-2-14 and T2-4A and with lethals 19Cc and 16- *F 3-98 (Fb). Normal with ot and bbl . Shows mell and mal with Df (1) mal10 . *F bk1: Between l (1) 19Cc and mal. Left limit from non-inclusion of 19Cc; right *F from *F inclusion of mal. *F bk2: Between eo and wap. Left limit from inclusion of E-54; right from non- *F inclusion of Dfs. B111 and 16-2-14. *F ___________________________________________________________________ *F Df (1) 16-3-95 *F Lethal with Dfs. B-12, B-57, 16-3-35 (Fb) A-118 and 17-137 (Fb) and with lethals *F E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, R-9-28, E-81 and AA-33. Viable *F with Dfs. B-111, mal-10, 17-25 (Fb) and 16-2-14 . Normal with ot and bbl. *F bk1: Between mell and run. Left limit from non inclusion of Df (1) 17-25; right *F from inclusion of AA-33. *F bk2: Between eo and wap. Left limit from inclusion of E-54; right from non- *F inclusion of Dfs. B-111 and 16-2-14. *F ___________________________________________________________________ *F Df (1)16-3-160 *F Lethal with Dfs. B-12, B-57, A-118, 16-3-35 and 17-137 (Fb) and with lethals E- *F 54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, R-9-28 and E-81. Viable with Dfs. *F B-111, 17-25 (Fb), 16-2-14 and mal-10 and with lethals AA-33 and 17-26 (Fb). *F Normal with bbl and ot. *F bk1: Between run and l (1) E-81. Left limit fron non-inclusion of l (1) AA-33; *F right *F from inclusion of E-81. *F bk2: Between eo and wap. Left limit from inclusion of E-54; right from non- *F inclusion of Dfs. B-111 and 16-2-14. *F ___________________________________________________________________ *F Df (1)16-3-53 and Df (1) 17-456 *F Lethal with Dfs. B-12, B-57 and 16-3-35 and with lethals E-81 and AA-33. Viable *F with Dfs. mal-10, 17-351 (Fb), A-118, 17-137 (Fb), 17-25 (Fb), 16-2-14 and B-111 *F and with lethals E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1 and R-9-28. *F Normal with bbl and ot. Df (1) 16-3-53 and Df (1) 17-456/Dp (1;Y) mal+ males are *F sterile. *F bk1: Between mell and run. Left limit from non-inclusion of Df (1) 17-25; right *F from inclusion of l (1) AA-33. *F bk2: Between l (1) E-81 and l (1) R-9-28. Left limit from inclusion of E-81; *F right *F from non-inclusion of R-9-28. *F ___________________________________________________________________ *F Df (1) 16-2-5 *F Df (1) 16-2-5/Dp (1;Y) mal \+ males are sterile. Lethal with Df (1) 17-25 (Fb). *F Viable with Dfs. B-111, B-12, 16-3-35 (Fb), 17-322 and T2-4A and with lethals *F AA-33, 16-1-27 (Fb), 17-234 (Fb). Not tested with l (1) 19 Cc. Not tested with *F l(1) 16-3-98 (Fb) (19-Cb1) because in both cases the males are sterile. Do not *F show *F mel in combination with Df (1) T2-4A . Do not show mal with Df (1) 17-322. *F bk1: Left limit from non-inclusion of mel; right undetermined. *F bk2: Left limit undetermined, right from non inclusion of mal. *F ___________________________________________________________________ *F l (1) 16-1-49 *F Lethal with Dfs. B-111 and 17-439 (Fb) and with l (1) x-3. Viable with Dfs. *F B-57, *F B-12, 16-3-35 (Fb), 17-25 (Fb), 17-274 (Fb) and 18-76 and with lethals A-200, Q- *F 56, Q-456, 114, P-19, Q-463, x-4, R-9-18, 3-Des. Normal with bbl. Covered by Dp *F (1;Y) BS Y y+ *F Location: Allele of x-3 (stnA). *F ___________________________________________________________________ *F l (1) 16-3-4 and l (1) 17-410 *F Lethal with Dfs. B-111 and 16-3-34 and with l (1) Q-56. Viable with Dfs. B-57, *F B- *F 12, 16-3-35 (Fb), 17-25 (Fb) and 17-196, and with lethals A-200, Q-456, 114, *F x-3, *F P-19, Q-463, x-4, R-9-18 and 3-Des. Normal with bbl. Not covered by Dp (1;Y) *F BS Y y+. Both are male sterile with Dp (1;Y) mal+. *F Location: Male sterile alleles of l (1) Q-56 (intro ?) *F l (1) 17-344 *F Lethal with Dfs. B-12, B-57 and A-118 and with lethal R-9-28. Viable with Dfs. *F B- *F 111, 16-3-35 (Fb), 17-25 (Fb), 17-137 (Fb), 16-2-14 and T2-14A. and with lethals *F E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, E-81 and AA-33. Normal with ot *F and with bbl. *F Location: Allele of l (1) R-9-28. *F l (1) 16-2-9 *F Lethal with Dfs. B-111 and 16-3-34 and with lethals Q-56 and x-4. Viable with *F Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), 16-1-85 (Fb), 17-2-52 (Fb) and 17-19 *F and with lethals A-200, Q-456, 114, x-3, P-19, Q-463, R-9-18 and 3-Des. Normal *F with bbl. *F Location: Is a double lethal, one is allelic to l (1) Q-56 and the other to l *F (1) x-4 *F (sph). *F From ermunoz@cnea.gov.ar Mon Jan 19 12:44:30 2004 *F To: rd120@gen.cam.ac.uk *F Subject: Drosophila Df(1)17-25 *F Dear Rachel, *F I had, the apparently wrong idea, that Df(1)17-25 *F had been previously described in the Flybase, *F but when checking if somebody had done the *F cytology I couldn't find it in the FlyBase. Since I used it *F for mapping other deficiencies I think it is necessary *F to include the data I have: Is a large deficiency with *F similar breakpoints than Df(1)16-2-19 (described in *F Flybase). The only difference being that the *F latter is male sterile. *F Df(1)17-25 : *F Lethal with lethals ELC1, 25C4, 16-1-27, 16-3-98, 17-234 *F and LB11. Viable with Dfs. B-111, 16-3-95 and B-12 and *F with l(1)AA-33. It is normal with 'bbl'and shows 'mal' *F and 'mell' with Df(1)17-322. It shows 'mell' with *F Df(1)16-3-35. It shows visible with 'ot'. *F bk1: To the left of 'ot'. Left limit undetermined, *F right from inclusion of 'ot'. *F bk2:Left limit from inclusion of 'mell'; right from non- *F inclusion of lethal AA-33. *F In my previous mails I fail to clarify the following: *F You have surely noted that for mapping all the *F lethals many crosses appear as superfluous. *F However, the information recovered is relevant when *F mapping lethals induced by high LET radiations. In this *F case, and due to the crowded ionization tracks generated *F by neutrons it is necessary to clearly establish if the damage *F inflicted is a continuous deficiency or it actually consists *F of close double or multiple lethals in the same segment. *F Thus it has a bearing on the reported RBE for recessive *F lethals and the effect of ionization tracks generated by *F high LET radiations in general ( that are so different from *F those produced by ionization of lower LET like gamma or X rays). *F I apologize for bothering you so much but *F I thought it was relevant. Many thanks *F Enzo. # *U FBrf0173253 *a Kaufman *b T. *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Wed Jan 14 22:10:12 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: In(3R)pb36 and In(3R)Antp5Lpb36R *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (12/03). *F Revised breakpoints of In(3R)pb36 are 84A1-2;87B1-2. *F In(3R)Antp5Lpb36R was generated by recombination from the precursor *F aberrations In(3R)pb36 and In(3R)Antp5 as the reciprocal recombinant of *F In(3R)pb36LAntp5R (FBab0022429). It duplicates both 84A1-2;84B2 and *F 87B1-2;87C. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173254 *a Fujioka *b M. *c J. *d Jaynes *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 17:56:34 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{CQ2-GAL4} and P{RN2-GAL4} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Miki Fujioka and James Jaynes, Thomas Jefferson University *F (12/03). *F P{CQ2-GAL4}H and P{RN2-GAL4}P are homozygous viable and fertile, second *F chromosome insertions. *F P{CQ2-GAL4}O and P{RN2-GAL4}E are homozygous viable and fertile, third *F chromosome insertions. *F The construction and transformation of P{RN2-GAL4} was described in Fujioka *F et al. 2003, Development 130: 5385-5400. *F The construction and transformation of P{CQ2-GAL4} was described in *F Landgraf et al. 2003, PLoS Biology 1: 221-230. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173255 *a Fujioka *b M. *c J. *d Jaynes *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 18:00:41 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-tau-lacZ.B}3 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Miki Fujioka and James Jaynes, Thomas Jefferson University *F (12/03). *F P{UAS-tau-lacZ.B}3 is a homozygous viable and fertile third chromosome *F insertion. *F The construct and its transformation were described in Hidalgo et al. 1995 *F Development 121(11):3703--3712 *F (FBrf0084024). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173256 *a Frolov *b M. *t 2004.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 18:28:58 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: Rbf14 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Maxim Frolov, Massachusetts General Hospital (12/03). *F Rbf14 is a null allele Rbf. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173257 *a Kaufman *b T. *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 19:27:13 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{hsp70-ems}1 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (12/03). *F P{hsp70-ems}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173258 *a Kaufman *b T. *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 19:38:48 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{Hsp70-Ama} construct and insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Thom Kaufman, Indiana University (12/03). *F P{Hsp70-Ama} contains an Amalgam cDNA driven by the Hsp70 promoter. It was *F constructed in a pP{CaSpeR} vector. *F P{Hsp70-Ama}2 is a homozygous viable and fertile, second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173259 *a Ratnakumar *b K. *c C. *d Desplan *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 22:43:00 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: Rh-GAL4 and Rh-GFP insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York University *F (12/03). *F P{ninaE-EGFP.P}2, P{Rh3-GAL4}2, P{Rh3-EGFP.P}2, P{Rh4-EGFP.P}2, *F P{Rh5-EGFP.P}2, P{Rh5-GAL4}2, P{Rh6-GAL4.D}2 and P{Rh6-EGFP.P}2 are second *F chromosome insertions *F P{Rh4-EGFP.P}3, P{Rh6-EGFP.P}3 and P{Rh6-GAL4.D}3 are third chromosome *F insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173260 *a Andrade *b R. *c J. *d Deal *e K. *f Cook *t 2004.1.19 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 22:58:59 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(3R)BSC47 *F Isolation and Characterization of Df(3R)BSC47 *F Rachel Andrade, Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC47 was isolated as a P transposase-induced male recombination *F event involving P{EP}CG31549EP3625 and P{EP}CG2017EP3503. The deletion *F was isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{EP}CG31549EP3625/P{EP}CG2017EP3503 ca1 males. Polytene chromosome *F squashes showed the breakpoints 83B7-C1;83C6-D1. Df(3R)BSC47 failed to *F complement casj1C2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173261 *a Andrade *b R. *c K. *d Cook *t 2004.1.19 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 19 23:03:34 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and Characterization of Df(3R)BSC48 *F Cc: Rachel Andrade *F Isolation and Characterization of Df(3R)BSC48 *F Rachel Andrade and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC48 was isolated as a P transposase-induced male recombination *F event involving P{EP}EP3281 and P{PZ}l(3)0468404684. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{EP}EP3281/P{PZ}l(3)0468404684 ca1males. Polytene chromosome squashes *F showed the breakpoints 95A5-8;95B2-6. Df(3R)BSC48 failed to complement *F HmgcrC14.5. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173262 *a Cherbas *b L. *t 2004.1.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 20 15:46:17 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-DIAP1.H}3 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Lucy Cherbas, Indiana University. *F P{UAS-DIAP1.H}3 is a homozygous viable and fertile, third chromosome *F insertion originating in the laboratory of Bruce Hay. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173263 *a Bonini *b N. *t 2003.12 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 20 15:57:40 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{UAS-Hsc70-4.K71S} and P{UAS-Hsap\HSPA1L.W} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Nancy Bonini, University of Pennsylvania (12/03). *F P{UAS-Hsc70-4.K71S}I is a homozygous viable and fertile, second chromosome *F insertion originating in the lab of Karen Palter. *F P{UAS-Hsap\HSPA1L.W}41.1 is a homozygous viable and fertile, third *F chromosome insertion. *F P{UAS-Hsap\HSPA1L.W}53.1 is second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173264 *a Deal-Herr *b M. *c K. *d Cook *t 2004.1.20 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Tue Jan 20 17:41:25 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: Isolation and characterization of Df(2R)BSC49 *F Cc: medeal@bio.indiana.edu *F Isolation and characterization of Df(2R)BSC49 *F Megan Deal-Herr and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC49 was isolated as a P transposase-induced male recombination *F event involving P{EP}EP2344 and P{EP}CG14478EP2283. The deletion was *F isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 *F females X cn1 P{EP}CG14478EP2283/P{EP}EP2344 bw1 sp1; TMS, Sb1 *F P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 53D9-E1;54B5-10. Df(2R)BSC49 failed to complement *F RhoGEF204291, cnkk16314, mmk01212 and cnkE-2083. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173265 *a Ratnakumar *b K. *c C. *d Desplan *t 2004.1.26 *T personal communication to FlyBase *u *F From kcook@bio.indiana.edu Mon Jan 26 14:49:04 2004 *F To: flybase-updates@morgan.harvard.edu *F Subject: P{longGMR-GAL4}3 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York University *F (12/03). *F P{longGMR-GAL4}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-855-5782 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0173266 *a Montell *b D. *t 2004.1.30 *T personal communication to FlyBase *u *F To: Rachel Drysdale *F Date: Fri, 30 Jan 2004 11:16:59 \-0500 *F From: Denise Montell *F Subject: Re: RhoX vs. RhoBTB *F Yes, they are the same gene. *F On Friday, January 30, 2004 10:46 AM, Rachel Drysdale *F wrote: *F >Dear Denise, *F > *F >I enclose a query sent to FlyBase by Craig Magie, who raises the possibility *F >that RhoX (FBgn0041190) and RhoBTB (FBgn0036980) might be the same gene. The *F >only references we have for RhoX are yours, and judging from the gene record it *F >seems likely that you have unpublished sequence information about RhoX that *F >would answer this question. If you could either send me the sequence or BLAST *F >it against the RhoBTB sequence and let me know the bottom line then that would *F >be helpful in telling me whether or not to merge these gene *F >records. *F > *F >With best wishes, and thanks for your help, *F > *F >Rachel. *F > *F >------------- Begin Forwarded Message \------------- *F > *F >Envelope-to: rd120@gen.cam.ac.uk *F >Date: Thu, 29 Jan 2004 20:56:51 \-0500 (EST) *F >From: flybase-server@rail.bio.indiana.edu *F >To: flybase-help@morgan.harvard.edu *F >Subject: RhoX vs. RhoBTB *F > *F > comments: Hello, *F > *F > I am working on the Rho1 small GTPase. In the course of writing my PhD *F >thesis, I've been researching the various Rho family members in Drosophila. *F >There are 2 flybase entries, RhoX and RhoBTB, which look to me like they *F >actually represent the same gene. RhoX was mapped cytologically, with no *F >molecular information, and the only Rho-like gene in this interval is RhoBTB. *F >Is it true that these are the same gene? *F > *F > thanks very much, *F > Craig Magie *F > mailto: flybase-help@morgan.harvard.edu *F > realname: Craig Magie *F > reply-to: cmagie@fhcrc.org *F > *F >Sent from computer 140.107.17.250 *F > *F > *F >------------- End Forwarded Message \------------- # *U FBrf0173267 *a Flores *b C. *t 2004.1.28 *T personal communication to FlyBase *u *F To: FlyBase *F Date: Wed, 28 Jan 2004 16:44:51 \-0600 *F From: Carlos Flores *F Subject: data on deletions near 50C *F Cc: Bill Engels , *F 'Christine R. Preston' , *F Chuck Carey *F RE: 50C molecular analysis of deletions et al. *F I have characterized the endpoints of eight of the deletions in the 50C region *F created by Christine R. Preston in the Engels' lab ( Preston et al., 1996 *F Flanking duplications and deletions associated with P-induced male *F recombination in Drosophila. Genetics 144(4): 1623--1638  FBrf0091145). The *F deletions were caused by 'HEI', hybrid element insertion, an aberrant effect *F of P element mobilization. These eight deletions retain a CaSpeR element at *F the original insertion site but have flanking deletions extending proximally *F or distally. I used TAIL PCR (Liu YG, Whittier RF. 'Thermal asymmetric *F interlaced PCR: automatable amplification and sequencing of insert end *F fragments from P1 and YAC clones for chromosome walking.' Genomics. 1995 Feb *F 10; 25(3): 674-81) to amplify the (unknown) deletion junctions and then *F sequenced the resulting products. I also used TAIL PCR to determine the *F position of the P element insertion in the peanuts (pea1) mutant stock. The *F data are listed below. *F For reference, the starting element, P{CaSpeR}Cp150C (FBti0004219) which *F remains at the left or right boundary of all these deletions has the 8 bp *F target duplication of GATTGGGC corresponding to nucleotide position 9029183 to *F 9029190 of the sequence of chromosome arm 2R release:3.1 *F Df(2R)50C-36 *F FBab0026827 *F The deletion extends ca. 43 kb distally from the end of P{CaSpeR}Cp150C. *F The sequence flanking the 3' end of the P element was determined approximately *F as tcgtcgccggcgatttttta, corresponding to nucleotide position 9071823 of the *F sequence of chromosome arm 2R release:3.1 *F Deletes from the middle of Cp1 to near the start of shorter mam transcripts *F and all the genes between. *F Deletes part of Cp1, deletes all of Aats-phe, CG6704, CG18568 CG6701, CG13350, *F CG8067, RN-tre, CG30482 and part of mam. *F Df(2R)50C-38 *F FBab0026828 *F The deletion extends ca. 211 kb distally from the end of P{CaSpeR}Cp150C. *F The sequence flanking the 3' end of the P element was determined exactly as *F cgccacacgctttatgtaac, corresponding to nucleotide position 9239710 of the *F sequence of chromosome arm 2R release:3.1 *F Deletes from the middle of Cp1 into the middle of cg and all the genes between. *F Deletes part of Cp1, deletes all of Aats-phe, CG6704, CG18568 CG6701, CG13350, *F CG8067, RN-tre, CG30482, mam, CG18371, Prosap, CG13353, CG8233, CG8241, *F CG13018, CG13016, CG8257, O-fut1, CG8309, CG8323, CG18327, CG18324, CG8331, *F mRpS16, and part of cg. *F Df(2R)50C-40 *F FBab0026829 *F The deletion extends ca. 44 kb distally from the end of P{CaSpeR}Cp150C. *F The sequence flanking the 3' end of the P element was determined exactly as *F gtacacagcataacataatt, corresponding to nucleotide position 9073066 of the *F sequence of chromosome arm 2R release:3.1 *F Deletes from the middle of Cp1 to near the start of shorter mam transcripts *F and all the genes between. *F Deletes part of Cp1, deletes all of Aats-phe, CG6704, CG18568 CG6701, CG13350, *F CG8067, RN-tre, CG30482 and part of mam. *F Df(2R)50C-68 *F FBab0026833 *F The deletion extends ca. 6.7 kb proximally from the end of P{CaSpeR}Cp150C. *F The sequence immediately flanking the 5' end of the P element was determined *F approximately as aaaaaaacatacaggctgcc, corresponding to nucleotide position *F 9022521 of the sequence of chromosome arm 2R release:3.1 *F Deletes part of Cp1, all of CG13349 and into the longer transcripts of AGO1. *F Df(2R)50C-81 *F FBab0026836 *F The deletion extends ca. 7.3 kb proximally from the end of P{CaSpeR}Cp150C. *F The sequence immediately flanking the 5' end of the P element was determined *F roughly as tacaaaattatgaatctttc, corresponding to nucleotide position 9021881 *F of the sequence of chromosome arm 2R release:3.1 *F Deletes part of Cp1, all of CG13349 and into the longer transcripts of AGO1. *F Df(2R)50C-85 *F FBab0026837 *F The deletion extends ca. 7.6 kb proximally from the end of P{CaSpeR}Cp150C. *F The sequence immediately flanking the 5' end of the P element was determined *F approximately as atatccccgccatccacgtc, corresponding to nucleotide position *F 9021603 of the sequence of chromosome arm 2R release:3.1 *F Deletes part of Cp1, all of CG13349 and into the longer transcripts of AGO1. *F Df(2R)50C-102 *F FBab0026816 *F The deletion extends ca. 10.2 kb proximally from the end of P{CaSpeR}Cp150C. *F The sequence immediately flanking the 5' end of the P element was determined *F approximately as gacattatttatgttttttt, corresponding to nucleotide position *F 9019018 of the sequence of chromosome arm 2R release:3.1 *F Deletes part of Cp1, all of CG13349, most of CG33184 and into the longer *F transcripts of AGO1. *F Df(2R)50C-129 *F FBab0026821 *F The deletion extends ca. 27 kb distally from the end of P{CaSpeR}Cp150C. *F The sequence flanking the 3' end of the P element was determined exactly as *F gttttagcgctcgaaaattg, corresponding to nucleotide position 9055955 of the *F sequence of chromosome arm 2R release:3.1 *F Deletes from the middle of Cp1 to near the start of longer mam transcripts and *F all the genes between. *F Deletes part of Cp1, deletes all of Aats-phe, CG6704, CG18568 CG6701, CG13350, *F CG8067, RN-tre and probably part of mam. *F P{A92}pea1 *F FBti0003542 *F This P element is inserted in the 5' end of CG8241 but also in CG8233. *F The sequence immediately flanking the 3' end of the P element was determined *F exactly as AGCTGAACTAGTACTCATCG. Bases 1 to 8 of this sequence correspond to *F the 8 bp target duplication which are at nucleotide position 9217995 to *F 9218002 of the sequence of chromosome arm 2R release:3.1 *F N.B. there is a sequence polymorphism at positions 17 & 18 of the sequence *F given above relative to the release:3.1 reference sequence, which is *F agctgaactagtactctccg. *F The mutant phenotype of pea could result from disruption of either CG8241 or *F CG8233, but my guess would be that the male sterility is caused by disruption *F of CG8241 because it appears to be an RNA helicase/pre-mRNA splicing factor. # *U FBrf0173284 *a Torrie *b L.S. *t 2004.1.22 *T personal communication to FlyBase *u BDGP genome annotation. *F File deposited: Torrie.2004.1.22.doc ; File date: 2004.1.22 ; File size: 16384 *F ; File format: doc *F Subject: BDGP genome annotation *F From: 'Leah Suzanne Torrie' <9902470T@student.gla.ac.uk> *F To: flybase-updates@morgan.harvard.edu *F Date: Thu, 22 Jan 2004 11:26:23 \+0000 *F Please find attached a word document explaining my arguments for the *F annotation of eight Drosophila genes as members of the organic anion *F transporting polypeptide (oatp) family. *F Thank you, *F Leah Torrie.r *F Leah Torrie *F IBLS, Division of Molecular Genetics, *F University of Glasgow *F 56 Dumbarton Road *F Glasgow. *F G11 6NU. # *U FBrf0174215 *a FlyBase Curators *b ?. *c ?. *d Swiss-Prot Project Members *e ?. *f InterPro Project Members *t 2004- *T personal communication to FlyBase *u Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. *F For annotations documented via this citation, GO terms were assigned to *F FlyBase genes through InterPro protein domain assignments. *F *F InterPro protein domains are assigned to FlyBase genes as part of an *F ongoing collaboration between the UniProt database and FlyBase. The *F translation table of InterPro protein domains to GO terms (generated by *F Nicola Mulder at the EBI) was used to associate GO terms with FlyBase *F genes. *F *F Before addition to FlyBase, the InterPro-predicted GO annotations were *F filtered to prevent redundant annotations; 'molecular_function unknown *F ; GO:0005554' annotations were removed from the InterPro-predicted set *F of GO terms. InterPro-predicted GO terms that were identical to an *F existing non-IEA GO annotation for a FlyBase gene were not added in. *F InterPro-predicted GO terms that are a parent of (i.e. less specialized *F than) an existing non-IEA GO annotation for a gene were also not added *F in. All remaining InterPro GO predictions (including predicted GO terms *F that are identical to an existing IEA GO annotation for a given gene) *F were added into FlyBase, supported by the inferred from electronic *F (IEA) evidence code. # *U FBrf0174216 *a Ashburner *b M. *t 2004.4.1 *T personal communication to FlyBase *u *F From ClustalW alignments new-Stalker is Stalker2. *F fly3L_18659693 ----------TGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATATAAGTAA 50 *F flyX_1711713 ----------TGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATATAAGTAA 50 *F fly3R_3555248 GAAAGGAAATTGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATATAAGTAA 60 *F fly3L_14774061 ----------TGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATATAAGTAA 50 *F STALKER2 ----------TGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATATAAGTAA 50 *F \************************************************** *F *F fly3L_18659693 CGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCAAAGACC 110 *F flyX_1711713 CGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCAAAGACC 110 *F fly3R_3555248 CGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCAAAGACC 120 *F fly3L_14774061 CGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCAAAGACC 110 *F STALKER2 CGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCAAAGACC 110 *F \************************************************************ *F *F fly3L_18659693 ACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACATAAGCC 170 *F flyX_1711713 ACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACATAAGCC 170 *F fly3R_3555248 ACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACATAAGCC 180 *F fly3L_14774061 ACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACATAAGCC 170 *F STALKER2 ACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACATAAGCC 170 *F \************************************************************ *F *F fly3L_18659693 GATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGCCATACA 230 *F flyX_1711713 GATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGCCATACA 230 *F fly3R_3555248 GATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGCCATACA 240 *F fly3L_14774061 GATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGCCATACA 230 *F STALKER2 GATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGCCATACA 230 *F \************************************************************ *F *F fly3L_18659693 TAACATATGTATGCCTTCTGCATACACATGTATATGTATATACAA---------TATGTA 281 *F flyX_1711713 TAACATATGTATGCCTTCTGCATACACATGTATATGTATATACAA---------TATGTA 281 *F fly3R_3555248 TAACATATGTATGCCTTCTGCATACACATGTATATGTATATACAA---------TATGTA 291 *F fly3L_14774061 TAACATATGTATGCCTTCTGCATACACATGTATATGTATATACAATATATACAATATGTA 290 *F STALKER2 TAACATATGTATGCCTTCTGCATACACATGTATATGTATATACAA---------TATGTA 281 *F \********************************************* \****** *F *F fly3L_18659693 CAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGGATTTCA 341 *F flyX_1711713 CAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGAATT-CA 340 *F fly3R_3555248 CAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGAATT-CA 350 *F fly3L_14774061 CAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGGATT-CA 349 *F STALKER2 CAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGGATT-CA 340 *F \***************************************************** \*** \** *F *F fly3L_18659693 TTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATTACACTG 401 *F flyX_1711713 TTCAAATAAAACGATTCAAACGAAACAGACGCTCTGAGCTATTCAATATCTATTACACTG 400 *F fly3R_3555248 TTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATTACACTG 410 *F fly3L_14774061 TTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATTACACTG 409 *F STALKER2 TTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATTACACTG 400 *F \********************** \************************************* *F *F fly3L_18659693 AGCTATTACTTATTACTTATTACATGGCGACCGTGACTTGGTCTCGAGTCTGCCTCTGTG 461 *F flyX_1711713 AGCTATTACTTATTACTTATTACATGGCGACCGTGACTTGGTCTCGAGTCTGCCTCTGTG 460 *F fly3R_3555248 AGCTATTACTTATTACTTATTACATGGCGACCGTGACTTGGTCTCGAGTCTGCCTCTGTG 470 *F fly3L_14774061 AGCTATTACTTATTACTTATTACATGGCGACCGTGACTTGGTCTCGAGTCTGCCTCTGTG 469 *F STALKER2 AGCTATTACTTATTACTTATTACATGGCGACCGTGACTTGGTCTCGAGTCTGCCTCTGTG 460 *F \************************************************************ *F *F fly3L_18659693 TTGTGCCTCTGTGTATTGTGATTATGTATCGTGTATTGTGTTTGTGTTAAAAAAAAAAAA 521 *F flyX_1711713 TTGTGCCTCTGTGTATTGTGATTATGTATCGTGTATTGTGTTTGTGTTAAAAAAAAAAAA 520 *F fly3R_3555248 TTGTGCCTCTGTGTATTGTGATTATGTATCGTGTATTGTGTTTGTGTTAAAAAAAAAAAA 530 *F fly3L_14774061 TTGTGCCTCTGTGTATTGTGATTATGTATCGTGTATTGTGTTTGTGTTAAAAAAAAAAAA 529 *F STALKER2 TTGTGCCTCTGTGTATTGTGATTATGTATCGTGTATTGTGTTTGTGTTAAAAAAAAAAAA 520 *F \************************************************************ *F *F fly3L_18659693 AAAAAACAACATTTTGTGCCTATTATATTCAACGTGTGAACACACAAACATAACTAAAAT 581 *F flyX_1711713 AAAAA-CAACATTTTGTGCCTATTATATTCAACGTGTGAACACACAAACATAACTAAAAT 579 *F fly3R_3555248 AAAAA-CAACATCTTGTGCCTATTATATTCAACGTGTGAACACACAAACATAACTAAAAT 589 *F fly3L_14774061 AAAA--CAACATTTTGTGCCTATTATATTCAACGTGTGAACACACAAACATAACTAAAAT 587 *F STALKER2 AAAAA-CAACATTTTGTGCCTATTATATTCAACGTGTGAACACACAAACATAACTAAAAT 579 *F \**** \****** \*********************************************** *F *F fly3L_18659693 GTACTTGTAGTACTTGGACGCATTAAATGCAAATATATGTTCAATTGAAACAAACAAAGC 641 *F flyX_1711713 GTACTTGTAGTACTTGGACGCATTAAATGCAAATATATGTTCAATTGAAACAAACAAAGC 639 *F fly3R_3555248 GTACTTGTAGTACTTGGACGCATTAAATGCAAATATATGTTCAATTGAAACAAACAAAGC 649 *F fly3L_14774061 GTACTTGTAGTACTTGGACGCATTAAATGCAAATATATGTTCAATTGAAACAAACAAAGC 647 *F STALKER2 GTACTTGTAGTACTTGGACGCATTAAATGCAAATATATGTTCAATTGAAACAAACAAAGC 639 *F \************************************************************ *F *F fly3L_18659693 ACAAAAACACACAAAAACACACACAAAAACGCACAAAAACACGCAAACACAGCACACACA 701 *F flyX_1711713 ACAAAAACACACAAAAACACACACAAAAACGCACAAAAACACGCAAACACAGCACACACA 699 *F fly3R_3555248 ACAAAAACACACAAAAACACACACAAAAACGCACAAAAACACGCAAACACAGCACACACA 709 *F fly3L_14774061 ACAAAAACACACAAAAACACACACAAAAACGCACAAAAACACGCAAACACAGCACACACA 707 *F STALKER2 ACAAAAACACACAAAAACACACACAAAAACGCACAAAAACACGCAAACACAGCACACACA 699 *F \************************************************************ *F *F fly3L_18659693 GAAGAACAAGCGGCGATTGATGGCAGACGAGCCACAATTCGCCAATGCACAACCACAAGT 761 *F flyX_1711713 GAAGAACAAGCGGCGATTGATGGCAGACGAGCCACAATTCGCCAATGCACAACCACAAGT 759 *F fly3R_3555248 GAAGAACAAGCGGCGATTGATGGCAGACGAGCCACAATTCGCCAATGCACAACCACAAGT 769 *F fly3L_14774061 GAAGAACAAGCGGCGATTGATGGCAGACGAGCCACAATTCGCCAATGCACAACCACAAGT 767 *F STALKER2 GAAGAACAAGCGGCGATTGATGGCAGACGAGCCACAATTCGCCAATGCACAACCACAAGT 759 *F \************************************************************ *F *F fly3L_18659693 GCAACGGGACCAGCCAACGCTAGAGGAAGCGTTGCGGTTAAACAACGCCGATGGACCACG 821 *F flyX_1711713 GCAACGGGACCAGCCAACGCTAGAGGAAGCGTTGCGGTTAAACAACGCCGATGGACCACG 819 *F fly3R_3555248 GCAACGGGACCAGCCAACGCTAGAGGAAGCGTTGCGGTTAAACAACGCCGATGGACCACG 829 *F fly3L_14774061 GCAACGGGACCAGCCAACGCTAGAGGAAGCGTTGCGGTTAAACAACGCCGATGGACCACG 827 *F STALKER2 GCAACGGGACCAGCCAACGCTAGAGGAAGCGTTGCGGTTAAACAACGCCGATGGACCACG 819 *F \************************************************************ *F *F *F fly3L_18659693 CCCACTCACAGTAGCTGAGTACCGGGCACGGCAGGGGAAGAAACAACTACGAAAGCACAA 881 *F flyX_1711713 CCCACTCACAGTAGCTGAGTACCGGGCACGGCAGGGGAAGAAACAACTACGAAAGCACAA 879 *F fly3R_3555248 CCCACTCACAGTAGCTGAGTACCGGGCACGGCAGGAGAAGAAACAACTACGAAAGCACAA 889 *F fly3L_14774061 CCCACTCACAGTAGCTGAGTACCGGGCACGGCAGGAGAAGAAACAACTACGAAAGCACAA 887 *F STALKER2 CCCACTCACAGTAGCTGAGTACCGGGCACGGCAGGAGAAGAAACAACTACGAAAGCACAA 879 *F \*********************************** \************************ *F *F fly3L_18659693 ACGCTCAGGACGGAGGATTAAACTACTACAACAACGCCGACTGGTCAAGGAAATGACCCA 941 *F flyX_1711713 ACGCTCAGGACGGAGGATTAAACTACTACAACAACGCCGACTGGTCAAGGAAATGACCCA 939 *F fly3R_3555248 ACGCTCAGGACGGAGGATTAAACTACTACAACAACGCCGACTGGTCAAGGAAATGACCCA 949 *F fly3L_14774061 ACGCTCAGGACGGAGGATTAAACTACTACAACAACGCCGACTGGTCAAGGAAATGACCCA 947 *F STALKER2 ACGCTCAGGACGGAGGATTAAACTACTACAACAACGCCGACTGGTCAAGGAAATGACCCA 939 *F \************************************************************ *F *F fly3L_18659693 GTTGGCAAAAGAAGAATCAGCACGACAACGCTACCAAGAGCGTTTGGAAGCCATTGAACA 1001 *F flyX_1711713 GTTGGCAAAAGAAGAATCAGCACGACAACGCTACCAAGAGCGTTTGGAAGCCATTGAACA 999 *F fly3R_3555248 GTTGGCAAAAGAAGAATCAGCACGACAACGCTACCAAGAGCGTTTGGAAGCCATTGAACA 1009 *F fly3L_14774061 GTTGGCAAAAGAAGAATCAGCACGACAACGCTACCAAGAGCGTTTGGAAGCCATTGAACA 1007 *F STALKER2 GTTGGCAAAAGAAGAATCAGCACGACAACGCTACCAAGAGCGTTTGGAAGCCATTGAACA 999 *F \************************************************************ *F *F fly3L_18659693 AGAACTTCGCCAAAGTGCGAAGACACGCAAACGGGCTGCTTAAATGCAAATGCCCCAATT 1061 *F flyX_1711713 AGAACTTCGCCAAAGTGCGAAGACACGCAAACGGGCTGCTTAAATGCAAATGCCCCAATT 1059 *F fly3R_3555248 AGAACTTCGCCAAAGTGCGAAGACACGCAAACGGGCTGCTTAAATGCAAATGCCCCAATT 1069 *F fly3L_14774061 AGAACTTCGCCAAAGTGCGAAGACACGCAAACGGGCTGCTTAAATGCAAATGCCCCAATT 1067 *F STALKER2 AGAACTTCGCCAAAGTGCGAAGACACGCAAACGGGCTGCTTAAATGCAAATGCCCCAATT 1059 *F \************************************************************ *F *F fly3L_18659693 TGCCCTAGAATTAAAATAAACCCAAGCC-GAGGCGGGAAACCGCTGCTTGAAAATACTAA 1120 *F flyX_1711713 TGCCCTAGAATTAAAATAAACCCAAGCC-GAGGCGGGAAACCGCTGCTTGAAAATACTAA 1118 *F fly3R_3555248 TGCCCTAGAATTAAAATAAACCCAAGCC-GAGGCGGGAAACCGCTGCTTGAAAATACTAA 1128 *F fly3L_14774061 TGCCCTAGAATTAAAATAAACCCAAGCC-GAGGCGGGAAACCGCTGCTTGAAAATACTAA 1126 *F STALKER2 TGCCCTAGAATTAAAATAAACCCAAGCCCGAGGCGGGAAACCGCTGCTTGAAAATACTAA 1119 *F \**************************** \******************************* *F *F fly3L_18659693 CAAAACTTTATGCTAGGCTTTATACTAAAAAATGTGGTTGGAGATTTTTTTTT--AAAAT 1178 *F flyX_1711713 CAAAACTTTATGCTAGGCTTTATACTAAAAAATGTGGTTGGAGATTTTTTTTTT-AAAAT 1177 *F fly3R_3555248 CAAAACTTTATGCTAGGCTTTATACTAAAAAATGTGGTTGGAGATTTTTTTTTT-AAAAT 1187 *F fly3L_14774061 CAAAACTTTATGCTAGGCTTTATACTAAAAAATGTGGTTGGAGATTTTTTTTTTTAAAAT 1186 *F STALKER2 CAAAACTTTATGCTAGGCTTTATACTAAAAAATGTGGTTGGAGATTTTTTTTTT-AAAAT 1178 *F \***************************************************** \***** *F *F fly3L_18659693 CCTATTGAATTGTAAATTGTGACTTGTGAGCCAACCACATTAGCACTATCAAATTTCCAT 1238 *F flyX_1711713 CCTATTGAATTGTAAATTGTGACTTGTGAGCCAACCACATTAGCACTATCAAATTTCCAT 1237 *F fly3R_3555248 CCTATTGAATTGTAAATTGTGACTTGTGAGCCAACCACATTAGCACTATCAAATTTCCAT 1247 *F fly3L_14774061 CCTATTGAATTGTAAATTGTGACTTGTGAGCCAACCACATTAGCACTATCAAATTTCCAT 1246 *F STALKER2 CCTATTGAATTGTAAATTGTGACTTGTGAGCCAACCACATTAGCACTATCAAATTTCCAT 1238 *F \************************************************************ *F *F fly3L_18659693 GTCTCTGCCACTGAGTATATATACTCAGTTGCAAATAATTTGTGTAACTTTAACAACTTT 1298 *F flyX_1711713 GTCTCTGCCACTGAGTATATATACTCAGTTGCAAATAATTTGTGTAACTTTAACAACTTT 1297 *F fly3R_3555248 GTCTCTGCCACTGAGTATATATACTCAGTTGCAAATAATTTGTGTAACTTTAACAACTTT 1307 *F fly3L_14774061 GTCTCTGCCACTGAGTATATATACTCAGTTGCAAATAATTTGTGTAACTTTAACAACTTT 1306 *F STALKER2 GTCTCTGCCACTGAGTATATATACTCAGTTGCAAATAATTTGTGTAACTTTAACAACTTT 1298 *F \************************************************************ *F *F fly3L_18659693 AAATTTTCTATTTCAAAAATAAAATTTTACATTTTAAATTTAAATGAATAACATTTTAAA 1358 *F flyX_1711713 AAATTTTCTATTTCAAAAATAAAATTTTACATTTTAAATTTAAATGAATAACATTTTAAA 1357 *F fly3R_3555248 AAATTTTCTATTTCAAAAATAAAATTTTACATTTTAAATTTAAATGAATAACATTTTAAA 1367 *F fly3L_14774061 AAATTTTCTATTTCAAAAATAAAATTTTACATTTTAAATTTAAATGAATAACATTTTAAA 1366 *F STALKER2 AAATTTTCTATTTCAAAAATAAAATTTTACATTTTAAATTTAAATGAATAACATTTTAAA 1358 *F \************************************************************ *F *F fly3L_18659693 AATGGATTAAAAAAAAAAAAAAAAAAAATAACTTTCTTAAAGAGAATTTTCTTTCTAAAA 1418 *F flyX_1711713 AATGGATTAAAAAAAAAAAA--------TAACTTTCTTAAAGAGAATTTTCTTTCTAAAA 1409 *F fly3R_3555248 AATGGATAAAAAAAAAAAAAAAAAA--ATAACTTTCTTAAAGAGAATTTTCTTTCTAAAA 1425 *F fly3L_14774061 AATGGATTAAAAAAAAAAAAAAA-----TAACTTTCTTAAAGAGAATTTTCTTTCTAAAA 1421 *F STALKER2 AATGGATTAAAAAAAAAAAAAAAA----TAACTTTCTTAAAGAGAATTTTCTTTCTAAAA 1414 *F \******* \************ \******************************** *F *F fly3L_18659693 ATACAAGAAAAAATAGATTTTTATTTTTCATATTATATATATAATAACATTATATATAAT 1478 *F flyX_1711713 ATACAAGAAAAAATAGATTTTTATTTTTCATATTATATATATAATAACATTATATATAAT 1469 *F fly3R_3555248 ATACAAGAAAAAATAGATTTTTATTTTTCATATTATATATATAATAACATTATATATAAT 1485 *F fly3L_14774061 ATACAAGAAAAAATAGATTTTTATTTTTCATATTATATATATAATAACATTATATATAAT 1481 *F STALKER2 ATACAAGAAAAAATAGATTTTTATTTTTCATATTATATATATAATAACATTATATATAAT 1474 *F \************************************************************ *F *F fly3L_18659693 ACTTCAAAAAAAAAA--CGTTTCATTGAATATATAAAAATATTCAACACAAATTAAAATA 1536 *F flyX_1711713 ACTTCAAAAAAAAAAA-CGTTTCATTGAATATATAAAAATATTCAACACAAATTAAAATA 1528 *F fly3R_3555248 ACTTCAAAAAAAA----CGTTTCATTGAATATATAAAAATATTCAACACAAATTAAAATA 1541 *F fly3L_14774061 ACTTCAAAAAAAAAAAACGTTTCATTGAATATATAAAAATATTCAACACAAATTAAAATA 1541 *F STALKER2 ACTTCAAAAAAAAAAA-CGTTTCATTGAATATATAAAAATATTCAACACAAATTAAAATA 1533 *F \************* \******************************************* *F *F fly3L_18659693 AATTTTCTCTTCAATTTAAAATTACACAAATCAAACAAAAATTAGAAATAAAATTAGGAA 1596 *F flyX_1711713 AATTTTCTCCTCAATTTAAAATTACACAAATCAAACAAAAATTAGAAATAAAATTAGGAA 1588 *F fly3R_3555248 AATTTTCTCTTCAATTTAAAATTACACAAATCAAACAAAAATTAGAAATAAAATTAGGAA 1601 *F fly3L_14774061 AATTTTCTCCTCAATTTAAAATTACACAAATCAAACAAAAATTAGAAATAAAATTAGGAA 1601 *F STALKER2 AATTTTCTCCTCAATTTAAAATTACACAAATCAAACAAAAATTAGAAATAAAATTAGGAA 1593 *F \********* \************************************************** *F *F fly3L_18659693 ACCTAATAAGTAAAACAATTATATTACATGTCATTAAAATTGAATGAGCATTAAAGATAA 1656 *F flyX_1711713 ACCTAATAAGTAAAACAATTATATTACATGTCATTAAAATTGAATGAGCATTAAAGATAA 1648 *F fly3R_3555248 ACCTAATAAGTAAAACAATTATATTACATGTCATTAAAATTGAATGAGCATTAAAGATAA 1661 *F fly3L_14774061 ACCTAATAAGTAAAACAATTATATTACATGTCATTAAAATTGAATGAGCATTAAAGATAA 1661 *F STALKER2 ACCTAATAAGTAAAACAATTATATTACATGTCATTAAAATTGAATGAGCATTAAAGATAA 1653 *F \************************************************************ *F *F fly3L_18659693 TTCCATACCGAAGATAGACCCCCTAAAGACAATCATTTCGGAACTGTCTACTATGTTAGA 1716 *F flyX_1711713 TTCCATACCGAAGATAGACCCCCTAAAGACAATCATTTCGGAACTGTCTACTATGTTAGA 1708 *F fly3R_3555248 TTCCATACCGAAGATAGAATCCCTAAAGACAATCATTTCGGAACTGTCTACTATGTTAGA 1721 *F fly3L_14774061 TTCCATACCGAAGATAGACCCCCTAAAGACAATCATTTCGGAACTGTCTACTATGTTAGA 1721 *F STALKER2 TTCCATACCGAAGATAGACCCCCTAAAGACAATCATTTCGGAACTGTCTACTATGTTAGA 1713 *F \****************** \**************************************** *F *F fly3L_18659693 CAAAAATTCCAATATAGCGGACTCCACCGCAAACGTAATCAATACAGTTCAGGTATCACA 1776 *F flyX_1711713 CAAAAATTCCAATATAGCGGACTCCACCGCAAACGTAATCAATACAGTTCAGGTATCACA 1768 *F fly3R_3555248 CAAAAATTCCAATATAGCGGACTCCACCGCAAACGTAATCAATACAGTTCAGGTATCACA 1781 *F fly3L_14774061 CAAAAATTCCAATATAGCGGACTCCACCGCAAACGTAATCAATACAGTTCAGGTATCACA 1781 *F STALKER2 CAAAAATTCCAATATAGCGGACTCCACCGCAAACGTAATCAATACAGTTCAGGTATCACA 1773 *F \************************************************************ *F *F fly3L_18659693 GCCCGAACTTAAAATAATTACGGTACTCCTCATAATAATAGTAGTACTGCTATGTGCGAG 1836 *F flyX_1711713 GCCCGAACTTAAAATAATTACGGTACTCCTCATAATAATAGTAGTACTGCTATGTGCGAG 1828 *F fly3R_3555248 GCCCGAACTTAAAATAATTACGGTACTCCTCATAATAATAGTAGTACTGCTATGTGCGAG 1841 *F fly3L_14774061 GCCCGAACTTAAAATAATTACGGTACTCCTCATAATAATAGTAGTACTGCTATGTGCGAG 1841 *F STALKER2 GCCCGAACTTAAAATAATTACGGTACTCCTCATAATAATAGTAGTACTGCTATGTGCGAG 1833 *F \************************************************************ *F *F fly3L_18659693 TATGATAACGAAATTGTACAAACTACATAACAGATGCCTCAAGAAGAAATACTTGAGTAA 1896 *F flyX_1711713 TATGATAACGAAATTGTACAAACTACATAACAGATGCCTCAAGAAGAAATACTTGAGTGA 1888 *F fly3R_3555248 TATGATAACGAAATTGTACAAACTACATAACAGATGCCTCAAGAAGAAATACTTGAGTAA 1901 *F fly3L_14774061 TATGATAACGAAATTGTACAAACTACATAACAGATGCCTCAAGAAGAAATACTTGAGTAA 1901 *F STALKER2 TATGATAACGAAATTGTACAAACTACATAACAGATGCCTCAAGAAGAAATACTTGAGTAA 1893 *F \********************************************************** \* *F *F fly3L_18659693 GGCATTGGACCTAGATAAGGTCTAAAGTAGTACCCTGCGTACAAATTTCGAATATTCAAT 1956 *F flyX_1711713 GGCATTGGACCTAGATAAGGTCTAAAGTAGTACCCTGCGTACAAATTTCGAATATTCAAT 1948 *F fly3R_3555248 GGCATTGGACCTAGATAAGGTCTAAAGTAGTACCCTGCGTACAAATTTCGAATATTCAAT 1961 *F fly3L_14774061 GGCATTGGACCTAGATAAGGTCTAAAGTAGTACCCTGCGTACAAATTTCGAATATTCAAT 1961 *F STALKER2 GGCATTGGACCTAGATAAGGTCTAAAGTAGTACCCTGCGTACAAATTTCGAATATTCAAT 1953 *F \************************************************************ *F *F *F fly3L_18659693 TAAATAGAAATTATCTTTACATATATATATAAAAAAAAAAAAAAAGAATATCAAACATAC 2016 *F flyX_1711713 TAAATAGAAATTATCTTTACATATATATATAAAAAAAAAAAAAA-GAATATCAAACATAC 2007 *F fly3R_3555248 TAAATAGAAATTATCTTTACATATATATATAAAAAAAAAAAA---GAATATCAAACATAC 2018 *F fly3L_14774061 TAAATAGAAATTATCTTTACATATATATATATAAAAAAAAAAAC--AATATCAAACCCAA 2019 *F STALKER2 TAAATAGAAATTATCTTTACATATATATATATAAAAAAAAAAAC--AATATCAAACCCAC 2011 *F \******************************* \********** \********** \* *F *F fly3L_18659693 TATAAATGAAATATTAAGTAGAGACCCCCACTTCGCAAGTAGACTATATTTTATACTTGC 2076 *F flyX_1711713 TATAAATGAAATATTAAGTAGAGACCCCCACTTCGCAAGTAGACTATATTTTATACTTGC 2067 *F fly3R_3555248 TATAAATGAAATATTAAGTAGAGACCCCCACTTCGCAAGTAGACTATATTTTATACTTGC 2078 *F fly3L_14774061 TA---------------------------------------------------------- 2021 *F STALKER2 TATAA------------------------------------------------------- 2016 *F \** *F *F fly3L_18659693 CGAGCAACCCGTAAGCCTTTACGAGTTGCAAATATTATACATTAACACCTACAACTGCGA 2136 *F flyX_1711713 CGAGCAACCCGTAAGCCTTTACGAGTTGCAAATATTATACATTAACACCTACAACTGCGA 2127 *F fly3R_3555248 CGAGCAACCCGTAAGCCTTTACGAGTTGCAAATATTATACATTAACACCTACAACTGCGA 2138 *F fly3L_14774061 ------------------------------------------------------------ *F STALKER2 ------------------------------------------------------------ *F *F *F fly3L_18659693 GTTCTTTCCAATGTTCGGGTTGGAAAGAGAACAAGAAATCATGGTATTAAATTCAATACC 2196 *F flyX_1711713 GTTCTTTCCAATGTTCGGGTTGGAAAGAGAACAAGAAATCATGGTATTAAATTCAATACC 2187 *F fly3R_3555248 GTTCTTTCCAATGTTCGGGTTGGAAAGAGAACAAGAAATCATGGTATTAAATTCAATACC 2198 *F fly3L_14774061 ------------------------------------------------------------ *F STALKER2 ------------------------------------------------------------ *F *F *F fly3L_18659693 AAATGTTATCGCAGAAGTTGTAGAAATATCAAACCCAATACTAGGGTCAACAGAAATAAT 2256 *F flyX_1711713 AAATGTTATCGCAGAAGTTGTAGAAATATCAAACCCAATACTAGGGTCAACAGAAATAAC 2247 *F fly3R_3555248 AAATGTTATCGCAGAAGTTGTAGAAATATCAAACCCAATACTAGGGTCAACAGAAATAAT 2258 *F fly3L_14774061 ----------------------------------------CTAGGGTCAACAGAAATAAT 2041 *F STALKER2 ----------------------------------------CTAGGGTCAACAGAAATAAT 2036 *F \******************* *F *F fly3L_18659693 AACAGTTCGTAAGAATTAGAGCATGGAATGGCATGAAATATGCACAACAATTAAAAATAT 2316 *F flyX_1711713 AACAGTTCGTAAGAATTAGAGCATGGAATGGCATGAAATATGCACAACAATTAAAAATAT 2307 *F fly3R_3555248 AACAGTTCGTAAGAATTAGAGCATGGAATGGCATGAAATATGCACAACAATTAAAAATAT 2318 *F fly3L_14774061 AACAGTTCGTAAGAATTAGAGCATGGAATGGCATGAAATATGCACAACAATTAAAAATAT 2101 *F STALKER2 AACAGTTGGTAAGAATTAGAGCATGGAATGGCATGAAATATGCACAACAATTAAAAATAT 2096 *F \******* \**************************************************** *F *F fly3L_18659693 TAAAACTAAATTCGATAAGACATATAAATGCTTATC-GCCCAATAGGCCTATACAGGCTG 2375 *F flyX_1711713 TAAAACTAAATTCGATAAGACATATAAATGCTTATC-GCCCAATAGGCCTATACAGGCTG 2366 *F fly3R_3555248 TAAAACTAAATTCGATAAGACATATAAATGCTTATC-GCCCAATAGGCCTATACAGGCTG 2377 *F fly3L_14774061 TAAAACTAAATTCGATAAGACATATAAATGCTTATC-GCCCAATAGGCCTATACAGGCTG 2160 *F STALKER2 TAAAACTAAATTCGATAAGACATATAAATGCTTATCTGCCCAATAGGCCTATACAGGCTG 2156 *F \************************************ \*********************** *F *F fly3L_18659693 AAACAATTAAAAAACATGCAACTACATTAGTCGACTGCTTTAATGAAGCACGAACACTAA 2435 *F flyX_1711713 AAACAATTAAAAAACATGCAACTACATTAGTCGACTGCTTTAATGAAGCACGAACACTAA 2426 *F fly3R_3555248 AAACAATTAAAAAACATGCATCTACATTAGTCGACTGCTTTAATGAAGCACGAACACTAA 2437 *F fly3L_14774061 AAACAATTAAAAAACATGCATCTACATTAGTCGACTGCTTTAATGAAGCACGAACACTAA 2220 *F STALKER2 AAACAATTAAAAAACATGCATCTACATTAGTCGACTGCTTTAATGAAGCACGAACACTAA 2216 *F \******************** \*************************************** *F *F fly3L_18659693 TATACGAACATAGAGAAACACTTAATTCTGAACACTGGTCTAAGTTATCAAGACTATTAA 2495 *F flyX_1711713 TATACGAACATAGAGAAACACTTAATTCTGAACACTGGTCTAAGTTATCAAGACTATTAA 2486 *F fly3R_3555248 TATACGAACATAGAGAAACACTTAATTCTGAACACTGGTCTAAGTTATCAAGACTATTAA 2497 *F fly3L_14774061 TATACGAACATAGAGAAACACTTAATTCTGAACACTGGTCTAAGTTATCAAGACTATTAA 2280 *F STALKER2 TATACGAACATAGAGAAACACTTAATTCTGAACACTGGTCTAAGTTATCAAGACTATTAA 2276 *F \************************************************************ *F *F fly3L_18659693 TCAAACTTCGATTAAACTTGATAGCAGTTAAAAGGAAATTTAGTTTGGAAATTTCAATTC 2555 *F flyX_1711713 TCAAACTTCGATTAAACTTGATAGCAGTTAAAAGGAAATTTAGTTTGGAAATTTCAATTC 2546 *F fly3R_3555248 TCAAACTTCGATTAAACTTGATAGCAGTTAAAAGGAAATTTAGTTTGGAAATTTCAATTC 2557 *F fly3L_14774061 TCAAACTTCGATTAAACTTGATAGCAGTTAAAAGGAAATTTAGTTTGGAAATTTCAATTC 2340 *F STALKER2 TCAAACTTCGATTAAACTTGATAGCAGTTAAAAGGAAATTTAGTTTGGAAATTTCAATTC 2336 *F \************************************************************ *F *F fly3L_18659693 CAACAATACTAAATACCCCTTTAACGATTGATACAGACGAACAATCTGAATCAATAGAAC 2615 *F flyX_1711713 CAACAATACTAAATACCCCTTTAACGATTGATACAGACGAACAATCTGAATCAATAGAAC 2606 *F fly3R_3555248 CAACAATACTAAATACCCCTTTAACGATTGATACAGACGAACAATCTGAATCAATAGAAC 2617 *F fly3L_14774061 CAACAATACTAAATACCCCTTTAACGATTGATACAGACGAACAATCTGAATCAATAGAAC 2400 *F STALKER2 CAACAATACTAAATACCCCTTTAACGATTGATACAGACGAACAATCTGAATCAATAGAAC 2396 *F \************************************************************ *F *F fly3L_18659693 CAGAAGAAATCGATTTAGAGGTCACCGAGTCGGAAGAAAAATATACCGACATCGAGGATA 2675 *F flyX_1711713 CAGAAGAAATCGATTTAGAGGTCACCGAGTCGGAAGAAAAATATACCGACATCGAGGATA 2666 *F fly3R_3555248 CAGAAGAAATCGATTTAGAGGTCACCGAGTCGGAAGAAAAATATACCGACATCGAGGATA 2677 *F fly3L_14774061 CAGAAGAAATCGATTTAGAGGTCACCGAGTCGGAAGAAAAATATACCGACATCGAGGATA 2460 *F STALKER2 CAGAAGAAATCGATTTAGAGGTCACCGAGTCGGAAGAAAAATATACCGACATCGAGGATA 2456 *F \************************************************************ *F *F fly3L_18659693 AAGACCTACTAAACCTAACAATTCCGGCTATATTAACATTAGCTGAAGACACTAATCAGG 2735 *F flyX_1711713 AAGACCTACTAAACCTAACAATTCCAGCTATATTAACATTAGCTGAAGACACTAATCAGG 2726 *F fly3R_3555248 AAGACCTACTAAACCTAACAATTCCAGCTATATTAACATTAGCTGAAGACACTAATCAGG 2737 *F fly3L_14774061 AAGACCTACTAAACCTAACAATTCCAGCTATATTAACATTAGCTGAAGACACTAATCAGG 2520 *F STALKER2 AAGACCTACTAAACCTAACAATTCCAGCTATATTAACATTAGCTGAAGACACTAATCAGG 2516 *F \************************* \********************************** *F *F fly3L_18659693 AAAAATTTAAAACAGAAAACATAATGG-CACAGTCCAATATTGATTTTCTGAATACAGCA 2794 *F flyX_1711713 AAAAATTTAAAACAGAAAACATAATGG-CACAGTCCAATATTGATTTTCTGAATACAGCA 2785 *F fly3R_3555248 AAAAATTTAAAACAGAAAACATAATGG-CACAGTCCAATATTGATTTTCTGAATACAGCA 2796 *F fly3L_14774061 AAAAATTTAAAACAGAAAACATAATGG-CACAGTCCAATATTGATTTTCTGAATACAGCA 2579 *F STALKER2 AAAAATTTAAAACAGAAAACATAATGGGCACAGTCCAATATTGATTTTCTGAATACAGCA 2576 *F \*************************** \******************************** *F *F fly3L_18659693 TCAAAGCTTATACCCGACTTTGACGGTAAGGCTGAAAACTTAACAAGTTTTATAGATGCT 2854 *F flyX_1711713 TCAAAGCTTATACCCGACTTTGACGGTAAGGCTGAAAACTTAACAAGTTTTATAGATGCT 2845 *F fly3R_3555248 TCAAAGCTTATACCCGACTTTGACGGTAAGGCTGAAAACTTAACAAGTTTTATAGATGCT 2856 *F fly3L_14774061 TCAAAGCTTATACCCGACTTTGACGGTAAGGCTGAAAACTTAACAAGTTTTATAGATGCT 2639 *F STALKER2 TCAAAGCTTATACCCGACTTTGACGGTAAGGCTGAAAACTTAACAAGTTTTATAGATGCT 2636 *F \************************************************************ *F *F fly3L_18659693 CTAAACATTGTAGATACAATCAAAGGTGAGCATGAGTCTCTAGCTGTTTCGGTTATAAAA 2914 *F flyX_1711713 CTAAACATTGTAGATACAATCAAAGGTGAGCATGAGTCTCTAGCTGTTTCGGTTATAAAA 2905 *F fly3R_3555248 CTAAACATTGTAGATACAATCAAAGGTGAGCATGAGTCTCTAGCTGTTTCGGTTATAAAA 2916 *F fly3L_14774061 CTAAACATTGTAGATACAATCAAAGGTGAGCATGAGTCTCTAGCTGTTTCGGTTATAAAA 2699 *F STALKER2 CTAAACATTGTAGATACAATCAAAGGTGAGCATGAGTCTCTAGCTGTTTCGGTTATAAAA 2696 *F \************************************************************ *F *F fly3L_18659693 ACCAAACTTAAAGGCCATGCAAGAAACCTCATAAGTAATGAGCAGACAATTGCTGCAATC 2974 *F flyX_1711713 ACCAAACTTAAAGGCCATGCAAGAAACCTCATAAGTAATGAGCAGACAATTGCTGCAATC 2965 *F fly3R_3555248 ACCAAACTTAAAGGCCATGCAAGAAACCTCATAAGTAATGAGCAGACAATTGCTGCAATC 2976 *F fly3L_14774061 ACCAAACTTAAAGGCCATGCAAGAAACCTCATAAGTAATGAGCAGACAATTGCTGCAATC 2759 *F STALKER2 ACCAAACTTAAAGGCCATGCAAGAAACCTCATAAGTAATGAGCAGACAATTGCTGCAATC 2756 *F \************************************************************ *F *F fly3L_18659693 ATTACCCAACTGTCAAGTGCAGTTAAAGGAGAATCGGTAGAAGTTATATCAGCTAAGCTT 3034 *F flyX_1711713 ATTACCCAACTGTCAAGTGCAGTTAAAGGAGAATCGGTAGAAGTTATATCAGCTAAGCTT 3025 *F fly3R_3555248 ATTACCCAACTGTCAAGTGCAGTTAAAGGAGAATCGGTAGAAGTTATATCAGCTAAGCTT 3036 *F fly3L_14774061 ATTACCCAACTGTCAAGTGCAGTTAAAGGAGAATCGGTAGAAGTTATATCAGCTAAGCTT 2819 *F STALKER2 ATTACCCAACTGTCAAGTGCAGTTAAAGGAGAATCGGTAGAAGTTATATCAGCTAAGCTT 2816 *F \************************************************************ *F *F fly3L_18659693 CTCAGTCTCCAACAAAAGAGTAAAACGGCCAACCAATACACCCAAGAGGTGGAGAAACTG 3094 *F flyX_1711713 CTCAGTCTCCAACAAAAGAGTAAAACGGCCAACCAATACACCCAAGAGGTGGAGAAACTG 3085 *F fly3R_3555248 CTCAGTCTCCAACAAAAGAGTAAAACGGCCAACCAATACACCCAAGAGGTGGAGAAACTG 3096 *F fly3L_14774061 CTCAGTCTCCAACAAAAGAGTAAAACGGCCAACCAATACACCCAAGAGGTGGAGAAACTG 2879 *F STALKER2 CTCAGTCTCCAACAAAAGAGTAAAACGGCCAACCAATACACCCAAGAGGTGGAGAAACTG 2876 *F \************************************************************ *F *F fly3L_18659693 ACAAAGGCTCTTGAAGGCGCCTATATCAGTGAAGGTTTAGGCCAAACTCTTGCCAATAAA 3154 *F flyX_1711713 ACAAAGGCTCTTGAAGGCGCCTATATCAGTGAAGGTTTAGGCCAAACTCTTGCCAATAAA 3145 *F fly3R_3555248 ACAAAGGCTCTTGAAGGCGCCTATATCAGTGAAGGTTTAGGCCAAACTCTTGCCAATAAA 3156 *F fly3L_14774061 ACAAAGGCTCTTGAAGGCGCCTATATCAGTGAAGGTTTAGGCCAAACTCTTGCCAATAAA 2939 *F STALKER2 ACAAAGGCTCTTGAAGGCGCCTATATCAGTGAAGGTTTAGGCCAAACTCTTGCCAATAAA 2936 *F \************************************************************ *F *F fly3L_18659693 TACAGCACTACCACAGCTGTAAAGGCCATGACGAAAAATTGCTCCATTGATAAGGTAAAA 3214 *F flyX_1711713 TACAGCACTACCACAGCTGTAAAGGCCATGACGAAAAATTGCTCCATTGATAAGGTAAAA 3205 *F fly3R_3555248 TACAGCACTACCACAGCTGTAAAGGCCATGACGAAAAATTGCTCCATTGATAAGGTAAAA 3216 *F fly3L_14774061 TACAGCACTACCACAGCTGTAAAGGCCATGACGAAAAATTGCTCCATTGATAAGGTAAAA 2999 *F STALKER2 TACAGCACTACCACAGCTGTAAAGGCCATGACGAAAAATTGCTCCATTGATAAGGTAAAA 2996 *F \************************************************************ *F *F fly3L_18659693 CTTATCATGCAAGCAGGCACATTCACAAACATGAATGATGCCATATCTAAATTTGTAAAC 3274 *F flyX_1711713 CTTATCATGCAAGCAGGCACATTCACAAACATGAATGATGCCATATCTAAATTTGTAAAC 3265 *F fly3R_3555248 CTTATCATGCAAGCAGGCACATTCACAAACATGAATGATGCCATATCTAAATTTGTAAAC 3276 *F fly3L_14774061 CTTATCATGCAAGCAGGCACATTCACAAACATGAATGATGCCATATCTAAATTTGTAAAC 3059 *F STALKER2 CTTATCATGCAAGCAGGCACATTCACAAACATGAATGATGCCATATCTAAATTTGTAAAC 3056 *F \************************************************************ *F *F fly3L_18659693 AGCTGCACGGAGATAACAGGTCAGAGTAACACTGTACTCTATTATCGACGAGGTGCAAAT 3334 *F flyX_1711713 AGCTGCACGGAGATAACAGGTCAGAGTAACACTGTACTCTATTATCGACGAGGTGCAAAT 3325 *F fly3R_3555248 AGCTGCACGGAGATAACAGGTCAGAGTAACACTGTACTCTATTATCGACGAGGTGCAAAT 3336 *F fly3L_14774061 AGCTGCACGGAGATAACAGGTCAGAGTAACACTGTACTCTATTATCGACGAGGTGCAAAT 3119 *F STALKER2 AGCTGCACGGAGATAACAGGTCAGAGTAACACTGTACTCTATTATCGACGAGGTGCAAAT 3116 *F \************************************************************ *F *F fly3L_18659693 AACAATAATAGAGGGGGCCGAGGTTATAATCGCGGCAGAAATGGCAACAATTACAACCGA 3394 *F flyX_1711713 AACAATAATAGAGGGGGCCGAGGTTATAATCGCGGCAGAAATGGCAACAATTACAACCGA 3385 *F fly3R_3555248 AACAATAATAGAGGGGGCCGAGGTTATAATCGCGGCAGAAATGGCAACAATTACAACCGA 3396 *F fly3L_14774061 AACAATAATAGAGGGGGCCGAGGTTATAATCGCGGCAGAAATGGCAACAATTACAACCGA 3179 *F STALKER2 AACAATAATAGAGGGGGCCGAGGTTATAATCGCGGCAGAAATGGCAACAATTACAACCGA 3176 *F \************************************************************ *F *F fly3L_18659693 GGCAACAATAATAGTAATAACTATAACAACCGTGGAGGAAGACGAGGCCGAAACCAAGGT 3454 *F flyX_1711713 GGCAACAATAATAGTAATAACTATAACAACCGTGGAGGAAGACGAGGCCGAAACCAAGGT 3445 *F fly3R_3555248 GGCAACAATAATAGTAATAACTATAACAACCGTGGAGGAAGACGAGGCCGAAACCAAGGT 3456 *F fly3L_14774061 GGCAACAATAATAGTAATAACTATAACAACCGTGGAGGAAGACGAGGCCGAAACCAAGGT 3239 *F STALKER2 GGCAACAATAATAGTAATAACTATAACAACCGTGGAGGAAGACGAGGCCGAAACCAAGGT 3236 *F \************************************************************ *F *F fly3L_18659693 AGAGGCCGCGGCAACTCCAACCAAGGTTACAATACCAACAATGTGAGAGTGACGCAAAAC 3514 *F flyX_1711713 AGAGGCCGCGGCAACTCCAACCAAGGTTACAATACCAACAATGTGAGAGTGACGCAAAAC 3505 *F fly3R_3555248 AGAGGCCGCGGCAACTCCAACCAAGGTTACAATACCAACAATGTGAGAGTGACGCAAAAC 3516 *F fly3L_14774061 AGAGGCCGCGGCAACTCCAACCAAGGTTACAATACCAACAATGTGAGAGTGACGCAAAAC 3299 *F STALKER2 AGAGGCCGCGGCAACTCCAACCAAGGTTACAATACCAACAATGTGAGAGTGACGCAAAAC 3296 *F \************************************************************ *F *F fly3L_18659693 ACGTCGGAAAACTCACAGACCCCTTTAGGAAACAATCAATAAATGCTAGAGTTCATTCCA 3574 *F flyX_1711713 ACGTCGGAAAACTCACAGACCCCTTTAGGAAACAATCAATAAATGCTAGAGTTCATTCCA 3565 *F fly3R_3555248 ACGTCGGAAAACTCACAGACCCCTTTAGGAAACAATCAATAAATGCTAGAGTTCATTCCA 3576 *F fly3L_14774061 ACGTCGGAAAACTCACAGACCCCTTTAGGAAACAATCAATAAATGCTAGAGTTCATTCCA 3359 *F STALKER2 ACGTCGGAAAACTCACAGACCCCTTTAGGAAACAATCAATAAATGCTAGAGTTCATTCCA 3356 *F \************************************************************ *F *F fly3L_18659693 TCAATTATAGTCTTAATATATTCG-TAACTTTTTACAATAATTCAAC-TGATAACAAGTT 3632 *F flyX_1711713 TCAATTATAGTCTTAATATATTCG-TAACTTTTTACAATAATTCAAC-TGATAACAAGTT 3623 *F fly3R_3555248 TCAATTATAGTCTTAATATATTCG-TAACTTTTTACAATAATTCAAC-TGATAACAAGTT 3634 *F fly3L_14774061 TCAATTATAGTCTTAATATATTCG-TAACTTTTTACAATAATTCAAC-TGATAACAAGTT 3417 *F STALKER2 TCAATTATAGTCTTAATATATTCGGTAACTTTTTACAATAATTCAACCTGATAACAAGTT 3416 *F \************************ \********************** \************ *F *F fly3L_18659693 AACATTTCTCATTGATACTGGTGCAGATATCTCACTTTTAAAAGTTAATTCAGATAACTT 3692 *F flyX_1711713 AACATTTCTCATTGATACTGGTGCAGATATCTCACTTTTAAAAGTTAATTCAGATAACTT 3683 *F fly3R_3555248 AACATTTCTCATTGATACTGGTGCAGATATCTCACTTTTAAAAGTTAATTCAGATAACTT 3694 *F fly3L_14774061 AACATTTCTCATTGATACTGGTGCAGATATCTCACTTTTAAAAGTTAATTCAGATAACTT 3477 *F STALKER2 AACATTTCTCATTGATACTGGTGCAGATATCTCACTTTTAAAAGTTAATTCAGATAACTT 3476 *F \************************************************************ *F *F fly3L_18659693 CAAAATTCAGGATGACAAAATAATAAACATCCAAGGCATAGGCCAAGGTGTAATAAAGTC 3752 *F flyX_1711713 CAAAATTCAGGATGACAAAATAATAAACATCCAAGGCATAGGCCAAGGTGTAATAAAGTC 3743 *F fly3R_3555248 CAAAATTCAGGATGACAAAATAATAAACATCCAAGGCATAGGCCAAGGTGTAATAAAGTC 3754 *F fly3L_14774061 CAAAATTCAGGATGACAAAATAATAAACATCCAAGGCATAGGCCAAGGTGTAATAAAGTC 3537 *F STALKER2 CAAAATTCAGGATGACAAAATAATAAACATCCAAGGCATAGGCCAAGGTGTAATAAAGTC 3536 *F \************************************************************ *F *F fly3L_18659693 TCAAGGAACAACCCTAATAGATCTTCAATCAACAAAATATATTATTCCACATGAATTTCA 3812 *F flyX_1711713 TCAAGGAACAACCCTAATAGATCTTCAATCAACAAAATATATTATTCCACATGAATTTCA 3803 *F fly3R_3555248 TCAAGGAACAACCCTAATAGATCTTCAATCAACAAAATATATTATTCCACATGAATTTCA 3814 *F fly3L_14774061 TCAAGGAACAACCCTAATAGATCTTCAATCAACAAAATATATTATTCCACATGAATTTCA 3597 *F STALKER2 TCAAGGAACAACCCTAATAGATCTTCAATCAACAAAATATATTATTCCACATGAATTTCA 3596 *F \************************************************************ *F *F fly3L_18659693 TTTGGTAAACTCAAATTTTTCAATACCATGTGATGGAATAATAGGCATTGACTTTATAAA 3872 *F flyX_1711713 TTTGGTAAACTCAAATTTTTCAATACCATGTGATGGAATAATAGGCATTGACTTTATAAA 3863 *F fly3R_3555248 TTTGGTAAACTCAAATTTTTCAATACCATGTGATGGAATAATAGGCATTGACTTTATAAA 3874 *F fly3L_14774061 TTTGGTAAACTCAAATTTTTCAATACCATGTGATGGAATAATAGGCATTGACTTTATAAA 3657 *F STALKER2 TTTGGTAAACTCAAATTTTTCAATACCATGTGATGGAATAATAGGCATTGACTTTATAAA 3656 *F \************************************************************ *F *F fly3L_18659693 AAAATTCAATTGCCAACTTGACTTCAAACCAAGTGAAGACTGGTTTATAATTAGACCCCA 3932 *F flyX_1711713 AAAATTCAATTGCCAACTTGACTTCAAACCAAGTGAAGACTGGTTTATAATTAGACCCCA 3923 *F fly3R_3555248 AAAATTCAATTGCCAACTTGACTTCAAACCAAGTGAAGACTGGTTTATAATTAGACCCCA 3934 *F fly3L_14774061 AAAATTCAATTGCCAACTTGACTTCAAACCAAGTGAAGACTGGTTTATAATTAGACCCCA 3717 *F STALKER2 AAAATTCAATTGCCAACTTGACTTCAAACCAAGTGAAGACTGGTTTATAATTAGACCCCA 3716 *F \************************************************************ *F *F fly3L_18659693 AAATTTAAATTATCCAATATATGTCCCAATAACATATAGCGCTGGCAACAATACAGTTCT 3992 *F flyX_1711713 AAATTTAAATTATCCAATATATGTCCCAATAACATATAGCGCTGGCAACAATACAGTTCT 3983 *F fly3R_3555248 AAATTTAAATTATCCAATATATGTCCCAATAACATATAGCGCTGGCAACAATACAGTTCT 3994 *F fly3L_14774061 AAATTTAAATTATCCAATATATGTCCCAATAACATATAGCGCTGGCAACAATACAGTTCT 3777 *F STALKER2 AAATTTAAATTATCCAATATATGTCCCAATAACATATAGCGCTGGCAACAATACAGTTCT 3776 *F \************************************************************ *F *F fly3L_18659693 TCTGCCAGCCAGATCACAAGTTATTCGGAAAATAGACATCAATAGCGTAAATGATCAAAT 4052 *F flyX_1711713 TCTGCCAGCCAGATCACAAGTTATTCGGAAAATAGACATCAATAGCGTAAATGATCAAAT 4043 *F fly3R_3555248 TCTGCCAGCCAGATCACAAGTTATTCGGAAAATAGACATCAATAGCGTAAATGATCAAAT 4054 *F fly3L_14774061 TCTGCCAGCCAGATCACAAGTTATTCGGAAAATAGACATCAATAGCGTAAATGATCAAAT 3837 *F STALKER2 TCTGCCAGCCAGATCACAAGTTATTCGGAAAATAGACATCAATAGCGTAAATGATCAAAT 3836 *F \************************************************************ *F *F fly3L_18659693 ATTCGTTCCTAATCAGGAAATACATAATGGAATTTATGTTGCGAATACAATAGCAGCATC 4112 *F flyX_1711713 ATTCGTTCCTAATCAGGAAATACATAATGGAATTTATGTTGCGAATACAATAGCAGCATC 4103 *F fly3R_3555248 ATTCGTTCCTAATCAGGAAATACATAATGGAATTTATGTTGCGAATACAATAGCAGCATC 4114 *F fly3L_14774061 ATTCGTTCCTAATCAGGAAATACATAATGGAATTTATGTTGCGAATACAATAGCAGCATC 3897 *F STALKER2 ATTCGTTCCTAATCAGGAAATACATAATGGAATTTATGTTGCGAATACAATAGCAGCATC 3896 *F \************************************************************ *F *F fly3L_18659693 AAAAAATGTATATATTCGACTTCTAAATACCACTAATTTCGACCAAATGGTCAAAGTAAA 4172 *F flyX_1711713 AAAAAATGTATATATTCGACTTCTAAATACCACTAATTTCGACCAAATGGTCAAAGTAAA 4163 *F fly3R_3555248 AAAAAATGTATATATTCGACTTCTAAATACCACTAATTTCGACCAAATGGTCAAAGTAAA 4174 *F fly3L_14774061 AAAAAATGTATATATTCGACTTCTAAATACCACTAATTTCGACCAAATGGTCAAAGTAAA 3957 *F STALKER2 AAAAAATGTATATATTCGACTTCTAAATACCACTAATTTCGACCAAATGGTCAAAGTAAA 3956 *F \************************************************************ *F *F fly3L_18659693 CAAAATCAAATATGAAAATCTTAAAGATTATGACATTCATAATACCAATCTAGAAGATAG 4232 *F flyX_1711713 CAAAATCAAATATGAAAATCTTAAAGATTATGACATTCATAATACCAATCTAGAAGATAG 4223 *F fly3R_3555248 CAAAATCAAATATGAAAATCTTAAAGATTATGACATTCATAATACCAATCTAGAAGATAG 4234 *F fly3L_14774061 CAAAATCAAATATGAAAATCTTAAAGATTATGACATTCATAATACCAATCTAGAAGATAG 4017 *F STALKER2 CAAAATCAAATATGAAAATCTTAAAGATTATGACATTCATAATACCAATCTAGAAGATAG 4016 *F \************************************************************ *F *F fly3L_18659693 AAGCGAAAAAGTACTATCATTACTGAAGAAGAATTTTCCAGAACAATTTAAAAGTCAATT 4292 *F flyX_1711713 AAGCGAAAAAGTACTATCATTACTGAAGAAAAATTTTCCAGAACAATTTAAAAGTCAATT 4283 *F fly3R_3555248 AAGCGAAAAAGTACTATCATTACTGAAGAAAAATTTTCCAGAACAATTTAAAAGTCAATT 4294 *F fly3L_14774061 AAGCGAAAAAGTACTATCATTACTGAAGAAAAATTTTCCAGAACAATTTAAAAGTCAATT 4077 *F STALKER2 AAGCGAAAAAGTACTATCATTACTGAAGAAGAATTTTCCAGAACAATTTAAAAGTCAATT 4076 *F \****************************** \***************************** *F *F fly3L_18659693 AACTGAATTATGCACAAAGTATAGTGATGTGTTCGGACTGGAAACCGAACC-AATATCAA 4351 *F flyX_1711713 AACTGAATTATGCACAAAGTATAGTGATGTGTTCGGACTGGAAACCGAACC-AATATCAA 4342 *F fly3R_3555248 AACTGAATTATGCACAAAGTATAGTGATGTGTTCGGACTGGAAACCGAACC-AATATCAA 4353 *F fly3L_14774061 AACTGAATTATGCACAAAGTATAGTGATGTGTTCGGACTGGAAACCGAACC-AATATCAA 4136 *F STALKER2 AACTGAATTATGCACAAAGTATAGTGATGTGTTCGGACTGGAAACCGAACCCAATATCAA 4136 *F \*************************************************** \******** *F *F fly3L_18659693 CAAATAATTTTTATAAACAAACATTAAGACTTAAAGATGATGAACCCATTTATATTAAGA 4411 *F flyX_1711713 CAAATAATTTTTATAAACAAACATTAAGACTTAAAGATGATGAACCCATTTATATTAAGA 4402 *F fly3R_3555248 CAAATAATTTTTATAAACAAACATTAAGACTTAAAGATGATGAACCCATTTATATTAAGA 4413 *F fly3L_14774061 CAAATAATTTTTATAAACAAACATTAAGACTTAAAGATGATGAACCCATTTATATTAAGA 4196 *F STALKER2 CAAATAATTTTTATAAACAAACATTAAGACTTAAAGATGATGAACCCATTTATATTAAGA 4196 *F \************************************************************ *F *F fly3L_18659693 ACTACAGAAGCCCGCATAGCCATATCGAAGAAATTCAAAAACAAGTAGGGAAATTAATTA 4471 *F flyX_1711713 ACTACAGAAGCCCGCATAGCCATATCGAAGAAATTCAAAAACAAGTAGGGAAATTAATTA 4462 *F fly3R_3555248 ACTACAGAAGCCCGCATAGCCATATCGAAGAAATTCAAAAACAAGTAGGGAAATTAATTA 4473 *F fly3L_14774061 ACTACAGAAGCCCGCATAGCCATATCGAAGAAATTCAAAAACAAGTAGGGAAATTAATTA 4256 *F STALKER2 ACTACAGAAGCCCGCATAGCCATATCGAAGAAATTCAAAAACAAGTAGGGAAATTAATTA 4256 *F \************************************************************ *F *F fly3L_18659693 ACGACAAAATCGTAGAACCATCTGTATCTGAATATAACAGCCCACTCTTGCTAGTTCCGA 4531 *F flyX_1711713 ACGACAAAATCGTAGAACCATCTGTATCTGAATATAACAGCCCACTCTTGCTAGTTCCGA 4522 *F fly3R_3555248 ACGACAAAATCGTAGAACCATCTGTATCTGAATATAACAGCCCACTCTTGCTAGTTCCGA 4533 *F fly3L_14774061 ACGACAAAATCGTAGAACCATCTGTATCTGAATATAACAGCCCACTCTTGCTAGTTCCGA 4316 *F STALKER2 ACGACAAAATCGTAGACCCATCTGTATCTGAATATAACAGCCCACTCTTGCTAGTTCCGA 4316 *F \**************** \******************************************* *F *F fly3L_18659693 AAAAATCATTACCGAATTCAGAACAAAAGAAATGGCGATTAGTAATTGACTATCGTCAAA 4591 *F flyX_1711713 AAAAATCATTACCGAATTCAGAACAAAAGAAATGGCGATTAGTAATTGACTATCGTCAAA 4582 *F fly3R_3555248 AAAAATCATTACCGAATTCAGAACAAAAGAAATGGCGATTAGTAATTGACTATCGTCAAA 4593 *F fly3L_14774061 AAAAATCATTACCGAATTCAGAACAAAAGAAATGGCGATTAGTAATTGACTATCGTCAAA 4376 *F STALKER2 AAAAATCATTACCGAATTCAGAACAAAAGAAATGGCGATTAGTAATTGACTATCGTCAAA 4376 *F \************************************************************ *F *F fly3L_18659693 TCAATAAGAAACTACTTTCTGATAAATTCCCACTCCCTAGAATTGATGACATTTTAGATC 4651 *F flyX_1711713 TTAATAAGAAACTACTTTCTGATAAATTCCCACTCCCTAGAATTGATGACATTTTAGATC 4642 *F fly3R_3555248 TTAATAAGAAACTACTTTCTGATAAATTCCCACTCCCTAGAATTGATGACATTTTAGATC 4653 *F fly3L_14774061 TTAATAAGAAACTACTTTCTGATAAATTCCCACTCCCTAGAATTGATGACATTTTAGATC 4436 *F STALKER2 TTAATAAGAAACTACTTTCTGATAAATTCCCACTCCCTAGAATTGATGACATTTTAGATC 4436 *F \* \********************************************************** *F *F fly3L_18659693 AACTAGGTCGAGCTAAATACTTTTCATGCCTTGACTTGATGTCAGGTTTCCATCAAATAG 4711 *F flyX_1711713 AACTAGGTCGAGCTAAATACTTTTCATGCCTTGACTTGATGTCAGGTTTCCATCAAATAG 4702 *F fly3R_3555248 AACTAGGTCGAGCTAAATACTTTTCATGCCTTGACTTGATGTCAGGTTTCCATCAAATAA 4713 *F fly3L_14774061 AACTAGGTCGAGCTAAATACTTTTCATGCCTTGACTTGATGTCAGGTTTCCATCAAATAG 4496 *F STALKER2 AACTAGGTCGAGCTAAATACTTTTCATGCCTTGACTTGATGTCAGGTTTCCATCAAATAG 4496 *F \*********************************************************** *F *F fly3L_18659693 AACTTGAAGAAAACTCAAGAAATATAACATCTTTTTCAACAAGCAATGGCTCATATCGCT 4771 *F flyX_1711713 AACTTGAAGAAAACTCAAGAAATATAACATCTTTTTCAACAAGCAATGGCTCATATCGCT 4762 *F fly3R_3555248 AACTTGAAGAAAACTCAAGAAATATAACATCTTTTTCAACAAGCAATGGCTCATATCGCT 4773 *F fly3L_14774061 AACTTGAAGAAAACTCAAGAAATATAACATCTTTTTCAACAAGCAATGGCTCATATCGCT 4556 *F STALKER2 AACTTGAAGAAAACTCAAGAAATATAACATCTTTTTCAACAAGCAATGGCTCATATCGCT 4556 *F \************************************************************ *F *F fly3L_18659693 TCACGCGATTACC-ATTTGGTCTCAAAATAGCACCAAATTCATTTCAGAGAATGATGACT 4830 *F flyX_1711713 TCACGCGATTACC-ATTTGGTCTCAAAATAGCACCAAATTCATTTCAGAGAATGATGACT 4821 *F fly3R_3555248 TCACGCGATTACC-ATTTGGTCTCAAAATAGCACCAAATTCATTTCAGAGAATGATGACT 4832 *F fly3L_14774061 TCACGCGATTACC-ATTTGGTCTCAAAATAGCACCAAATTCATTTCAGAGAATGATGACT 4615 *F STALKER2 TCACGCGATTACCCATTTGGTCTCAAAATAGCACCAAATTCATTTCAGAGAATGATGACT 4616 *F \************* \********************************************** *F *F fly3L_18659693 ATATCATTCTCTGGTTTAGAACCTTCTCAGGCATTCCTTTATATGGATGACTTAATGGTG 4890 *F flyX_1711713 ATATCATTCTCTGGTTTAGAACCTTCTCAGGCATTCCTTTATATGGATGACTTAATGGTG 4881 *F fly3R_3555248 ATATCATTCTCTGGTTTAGAACCTTCTCAGGCATTCCTTTATATGGATGACTTAATGGTG 4892 *F fly3L_14774061 ATATCATTCTCTGGTTTAGAACCTTCTCAGGCATTCCTTTATATGGATGACTTAATGGTG 4675 *F STALKER2 ATATCATTCTCTGGTTTAGAACCTTCTCAGGCATTCCTTTATATGGATGACTTAATGGTG 4676 *F \************************************************************ *F *F fly3L_18659693 ATAGGATGTTCCGAAAAACACATGATTAAAAACTTAACTGATGTTTTTAATATATGTAGG 4950 *F flyX_1711713 ATAGGATGTTCCGAAAAACACATGATTAAAAACTTAACTGATGTTTTTAATATATGTAGG 4941 *F fly3R_3555248 ATAGGATGTTCCGAAAAACACATGATTAAAAACTTAACTGATGTTTTTAATATATGTAGG 4952 *F fly3L_14774061 ATAGGATGTTCCGAAAAACACATGATTAAAAACTTAACTGATGTTTTTAATATATGTAGG 4735 *F STALKER2 ATAGGATGTTCCGAAAAACACATGATTAAAAACTTAACTGATGTTTTTAATATATGTAGG 4736 *F \************************************************************ *F *F fly3L_18659693 AAATATAACCTAAAGTTGCATCCGGAAAAATGTTCATTTTTCATGCATGAAGTGACATTC 5010 *F flyX_1711713 AAATATAACCTAAAGTTGCATCCGGAAAAATGTTCATTTTTCATGCATGAAGTGACATTC 5001 *F fly3R_3555248 AAATATAACCTAAAGTTGCATCCGGAAAAATGTTCATTTTTCATGCATGAAGTGACATTC 5012 *F fly3L_14774061 AAATATAACCTAAAGTTGCATCCGGAAAAATGTTCATTTTTCATGCATGAAGTGACATTC 4795 *F STALKER2 AAATATAACCTAAAGTTGCATCCGGAAAAATGTTCATTTTTCATGCATGAAGTGACATTC 4796 *F \************************************************************ *F *F fly3L_18659693 CTAGGTCACAAATGCACAGACAAAGG-AGTTTTGCCAGATGACAAAAAATATGACGTCAT 5069 *F flyX_1711713 CTAGGTCACAAATGCACAGACAAAGG-AGTTTTGCCAGATGACAAAAAATATGACGTCAT 5060 *F fly3R_3555248 CTAGGTCACAAATGCACAGACAAAGG-AGTTTTGCCAGATGACAAAAAATATGACGTCAT 5071 *F fly3L_14774061 CTAGGTCACAAATGCACAGACAAAGG-AGTTTTGCCAGATGACAAAAAATATGACGTCAT 4854 *F STALKER2 CTAGGTCACAAATGCACAGACAAAGGGAGTTTTGCCAGATGACAAAAAATATGACGTCAT 4856 *F \************************** \********************************* *F *F fly3L_18659693 CAAAAATTATCCTGTCCCTCACGATGCGGACAGCGCAAGACGATTTGTAGCATTCTGCAA 5129 *F flyX_1711713 CAAAAATTATCCTGTCCCTCACGATGCGGACAGCGCAAGACGATTTGTAGCATTCTGCAA 5120 *F fly3R_3555248 CAAAAATTATCCTGTCCCTCACGATGCGGACAGCGCAAGACGATTTGTAGCATTCTGCAA 5131 *F fly3L_14774061 CAAAAATTATCCTGTCCCTCACGATGCGGACAGCGCAAGACGATTTGTAGCATTCTGCAA 4914 *F STALKER2 CAAAAATTATCCTGTCCCTCACGATGCGGACAGCGCAAGACGATTTGTAGCATTCTGCAA 4916 *F \************************************************************ *F *F fly3L_18659693 CTATTATCGTCGATTTATAAAGAACTTCGCC-GACTATTCACGGCACATAACTAGATTAT 5188 *F flyX_1711713 CTATTATCGTCGATTTATAAAGAACTTCGCC-GACTATTCACGGCACATAACTAGATTAT 5179 *F fly3R_3555248 CTATTATCGTCGATTTATAAAGAACTTCGCC-GACTATTCACGGCACATAACTAGATTAT 5190 *F fly3L_14774061 CTATTATCGTCGATTTATAAAGAACTTCGCC-GACTATTCACGGCACATAACTAGATTAT 4973 *F STALKER2 CTATTATCGTCGATTTATAAAGAACTTCGCCTGACTATTCACGGCACATAACTAGATTAT 4976 *F \******************************* \**************************** *F *F fly3L_18659693 GTAAAAAGAATGTTCCTTTTGAATGGTCAAGCGAATGCCAAAACGCATTCGAATACCTTA 5248 *F flyX_1711713 GTAAAAAGAATGTTCCTTTTGAATGGTCAAGCGAATGCCAAAACGCATTCGAATACCTTA 5239 *F fly3R_3555248 GTAAAAAGAATGTTCCTTTTGAATGGTCAAGCGAATGCCAAAACGCATTCGAATACCTTA 5250 *F fly3L_14774061 GTAAAAAGAATGTTCCTTTTGAATGGTCAAGCGAATGCCAAAACGCATTCGAATACCTTA 5033 *F STALKER2 GTAAAAAGAATGTTCCTTTTGAATGGTCAAGCGAATGCCAAAACGCATTCGAATACCTTA 5036 *F \************************************************************ *F *F fly3L_18659693 AAGAAAAGCTTATGCACCCCACATTATTACAATATCCTGATTTTCGCAAAGAATTTTGCA 5308 *F flyX_1711713 AAGAAAAGCTTATGCACCCCACATTATTACAATATCCTGATTTTCGCAAAGAATTTTGCA 5299 *F fly3R_3555248 AAGAAAAGCTTATGCACCCCACATTATTACAATATCCTGATTTTCGCAAAGAATTTTGCA 5310 *F fly3L_14774061 AAGAAAAGCTTATGCACCCCACATTATTACAATATCCTGATTTTCGCAAAGAATTTTGCA 5093 *F STALKER2 AAGAAAAGCTTATGCACCCCACATTATTACAATATCCTGATTTTCGCAAAGAATTTTGCA 5096 *F \************************************************************ *F *F fly3L_18659693 TCATAACGGATGCTAGTAAACAAGCTTGTGGAGCGGTTTTAACCCAGAACCGAGACGGAA 5368 *F flyX_1711713 TCATAACGGATGCTAGTAAACAAGCTTGTGGAGCGGTTTTAACCCAGAACCGAGACGGAA 5359 *F fly3R_3555248 TCATAACGGATGCTAGTAAACAAGCTTGTGGAGCGGTTTTAACCCAGAACCGAGACGGAA 5370 *F fly3L_14774061 TCATAACGGATGCTAGTAAACAAGCTTGTGGAGCGGTTTTAACCCAGAACCGAGACGGAA 5153 *F STALKER2 TCATAACGGATGCTAGTAAACAAGCTTGTGGAGCGGTTTTAACCCAGAACCGAGACGGAA 5156 *F \************************************************************ *F *F fly3L_18659693 TTCAGATCCCAATATCTTATGCATCACGTTCGTTTACAAAAGGGGAAAGCAATAAGAGTA 5428 *F flyX_1711713 TTCAGCTCCCAATATCTTATGCATCACGTTCGTTTACAAAAGGGGAAAGCAATAAGAGTA 5419 *F fly3R_3555248 TTCAGCTCCCAATATCTTATGCATCACGTTCGTTTACAAAAGGGGAAAGCAATAAGAGTA 5430 *F fly3L_14774061 TTCAGCTCCCAATATCTTATGCATCACGTTCGTTTACAAAAGGGGAAAGCAATAAGAGTA 5213 *F STALKER2 TTCAGCTCCCAATATCTTATGCATCACGTTCGTTTACAAAAGGGGAAAGCAATAAGAGTA 5216 *F \***** \****************************************************** *F *F fly3L_18659693 CAACGGAACAAGAGTTAGCGGCAATTCATTGGGCAATTACCCATTTTAGACCATACATTT 5488 *F flyX_1711713 CAACGGAACAAGAGTTAGCGGCAATTCATTGGGCAATTACCCATTTTAGACCATACATTT 5479 *F fly3R_3555248 CAACGGAACAAGAGTTAGCGGCAATTCATTGGGCAATTACCCATTTTAGACCATACATTT 5490 *F fly3L_14774061 CAACGGAACAAGAGTTAGCGGCAATTCATTGGGCAATTACCCATTTTAGACCATACATTT 5273 *F STALKER2 CAACGGAACAAGAGTTAGCGGCAATTCATTGGGCAATTACCCATTTTAGACCATACATTT 5276 *F \************************************************************ *F *F fly3L_18659693 ATGGCAAACATTTCACAATCAAAACAGATCACAGACCTTTAACATATCTATTTTCTATGA 5548 *F flyX_1711713 ATGGCAAACATTTCACAATCAAAACAGATCACAGACCTTTAACATATCTATTTTCTATGA 5539 *F fly3R_3555248 ATGGCAAACATTTCACAATCAAAACAGATCACAGACCTTTAACATATCTATTTTCTATGA 5550 *F fly3L_14774061 ATGGCAAACATTTCACAATCAAAACAGATCACAGACCTTTAACATATCTATTTTCTATGA 5333 *F STALKER2 ATGGCAAACATTTCACAATCAAAACAGATCACAGACCTTTAACATATCTATTTTCTATGA 5336 *F \************************************************************ *F *F fly3L_18659693 CCAATCCCAATTCAAAATTAACTCGCATGCGACTAGAGCTTGAAGAATACGACTTCACTG 5608 *F flyX_1711713 CCAATCCCAATTCAAAATTAACTCGCATGCGACTAGAGCTTGAAGAATACGACTTCACTG 5599 *F fly3R_3555248 CCAATCCCAATTCAAAATTAACTCGCATGCGACTAGAGCTTGAAGAATACGACTTCACTG 5610 *F fly3L_14774061 CCAATCCCAGTTCAAAATTAACTCGCATGCGACTAGAGCTTGAAGAATACGACTTCACTG 5393 *F STALKER2 CCAATCCCAATTCAAAATTAACTCGCATGCGACTAGAGCTTGAAGAATACGACTTCACTG 5396 *F \********* \************************************************** *F *F fly3L_18659693 TAGAATACCTAAAGGGAAAAGATAATTTTGTGGCAGATGCACTGTCACGTATCACTATAA 5668 *F flyX_1711713 TAGAATACCTAAAGGGAAAAGATAATTTTGTGGCAGATGCACTGTCACGTATCACTATAA 5659 *F fly3R_3555248 TAGAATACCTAAAGGGAAAAGATAATTTTGTGGCAGATGCACTGTCACGTATCACTATAA 5670 *F fly3L_14774061 TAGAATACCTAAAGGGAAAAGATAATTTTGTGGCAGATGCACTGTCACGTATCACTATAA 5453 *F STALKER2 TAGAATACCTAAAGGGAAAAGATAATTTTGTGGCAGATGCACTGTCACGTATCACTATAA 5456 *F \************************************************************ *F *F fly3L_18659693 ACGAGCTGAAAGACATATCAGCAAATGTACTAAAAGTCACTACAAGACAGCAAAGTAAAC 5728 *F flyX_1711713 ACGAGCTGAAAGACATATCAGCAAATGTACTAAAAGTCACTACAAGACAGCAAAGTAAAC 5719 *F fly3R_3555248 ACGAGCTGAAAGACATATCAGCAAATGTACTAAAAGTCACTACAAGACAGCAAAGTAAAC 5730 *F fly3L_14774061 ACGAGCTGAAAGACATATCAGCAAATGTACTAAAAGTCACTACAAGACAGCAAAGTAAAC 5513 *F STALKER2 ACGAGCTGAAAGACATATCAGCAAATGTACTAAAAGTCACTACAAGACAGCAAAGTAAAC 5516 *F \************************************************************ *F *F fly3L_18659693 AGAAAAATATCTGCGCAGATACTAATATAAATAAACAAGAAGAAACTCTTGTTAACGCTT 5788 *F flyX_1711713 AGAAAAATATCTGCGCAGATACTAATATAAATAAACAAGAAGAAACTCTTGTTAACGCTT 5779 *F fly3R_3555248 AGAAAAATATCTGCGCAGATACTAATATAAATAAACAAGAAGAAACTCTTGTTAACGCTT 5790 *F fly3L_14774061 AGAAAAATATCTGCGCAGATACTAATATAAATAAACAAGAAGAAACTCTTGTTAACGCTT 5573 *F STALKER2 AGAAAAATATCTGCGCAGATACTAATATAAATAAACAAGAAGAAACTCTTGTTAACGCTT 5576 *F \************************************************************ *F *F fly3L_18659693 CTAAGCCCAACGTATATGAAGTCATTAATAATGACGAAATACGAAAAGTAGTGACTCTGC 5848 *F flyX_1711713 CTAAGCCCAACGTATATGAAGTCATTAATAATGACGAAATACGAAAAGTAGTGACTCTGC 5839 *F fly3R_3555248 CTAAGCCCAACGTATATGAAGTCATTAATAATGACGAAATACGAAAAGTAGTGACTCTGC 5850 *F fly3L_14774061 CTAAGCCCAACGTATATGAAGTCATTAATAATGACGAAATACGAAAAGTAGTGACTCTGC 5633 *F STALKER2 CTAAGCCCAACGTATATGAAGTCATTAATAATGACGAAATACGAAAAGTAGTGACTCTGC 5636 *F \************************************************************ *F *F fly3L_18659693 GAATAACTGAATCAAAATGTTTATTCAAACATGGAAATAAAGTTATAGCAAGAATTGATG 5908 *F flyX_1711713 GAATAACTGAATCAAAATGTTTATTCAAACATGGAAATAAAGTTATAGCAAGAATTGATG 5899 *F fly3R_3555248 GAATAACTGAATCAAAATGTTTATTCAAACATGGAAATAAAGTTATAGCAAGAATTGATG 5910 *F fly3L_14774061 GAATAACTGAATCAAAATGTTTATTCAAACATGGAAATAAAGTTATAGCAAGAATTGATG 5693 *F STALKER2 GAATAACTGAATCAAAATGTTTATTCAAACATGGAAATAAAGTTATAGCAAGAATTGATG 5696 *F \************************************************************ *F *F fly3L_18659693 TTAGCGATCTGTACACCAATGGAATTCTAGACTTAGGTCAGTTCTTCCAAAGGCTTGAAA 5968 *F flyX_1711713 TTAGCGATCTGTACACCAATGGAATTCTAGACTTAGGTCAGTTCTTCCAAAGGCTTGAAA 5959 *F fly3R_3555248 TTAGCGATCTGTACACCAATGGAATTCTAGACTTAGGTCAGTTCTTCCAAAGGCTTGAAA 5970 *F fly3L_14774061 TTAGCGATCTGTACACCAATGGAATTCTAGACTTAGGTCAGTTCTTCCAAAGGCTTGAAA 5753 *F STALKER2 TTAGCGATCTGTACACCAATGGAATTCTAGACTTAGGTCAGTTCTTCCAAAGGCTTGAAA 5756 *F \************************************************************ *F *F fly3L_18659693 CACAAGCCGGTATACATGAGATCAGCCAACTCAAAGTGGCACCGAGCGAAAAGATCTTTG 6028 *F flyX_1711713 CACAAGCCGGTATACATGAGATCAGCCAACTCAAAGTGGCACCGAGCGAAAAGATCTTTG 6019 *F fly3R_3555248 CACAAGCCGGTATACATGAGATCAGCCAACTCAAAGTGGCACCGAGCGAAAAGATCTTTG 6030 *F fly3L_14774061 CACAAGCCGGTATACATGAGATCAGCCAACTCAAAGTGGCACCGAGCGAAAAGATCTTTG 5813 *F STALKER2 CACAAGCCGGTATACATGAGATCAGCCAACTCAAAGTGGCACCGAGCGAAAAGATCTTTG 5816 *F \************************************************************ *F *F fly3L_18659693 AAACAATTTCAATAGACTCTTTTAAAAAAATGGGCAATAAATTATTGAAAATATTGAGAG 6088 *F flyX_1711713 AAACAATTTCAATAGACTCTTTTAAAAAAATGGGCAATAAATTATTGAAAATATTGAGAG 6079 *F fly3R_3555248 AAACAATTTCAATAGACTCTTTTAAAAAAATGGGCAATAAATTATTGAAAATATTGAGAG 6090 *F fly3L_14774061 AAACAATTTCAATAGACTCTTTTAAAAAAATGGGCAATAAATTATTGAAAATATTGAGAG 5873 *F STALKER2 AAACAATTTCAATAGACTCTTTTAAAAAAATGGGCAATAAATTATTGAAAATATTGAGAG 5876 *F \************************************************************ *F *F fly3L_18659693 TAGC-GCTACTCAAGCCGGTGACCCAAATATATGATCAAAAAGATCAAGAAGCGATACTG 6147 *F flyX_1711713 TAGC-GCTACTCAAGCCGGTGACCCAAATATATGATCAAAAAGATCAAGAAGCGATACTG 6138 *F fly3R_3555248 TAGC-GCTACTCAAGCCGGTGACCCAAATATATGATCAAAAAGATCAAGAAGCGATACTG 6149 *F fly3L_14774061 TAGC-GCTACTCAAGCCGGTGACCCAAATATATGATCAAAAAGATCAAGAAGCGATACTG 5932 *F STALKER2 TAGCCGCTACTCAAGCCGGTGACCCAAATATATGATCAAAAAGATCAAGAAGCGATACTG 5936 *F \**** \******************************************************* *F *F fly3L_18659693 TTTACATACCATGACGATCCAATTCAAGGAGGTCATACAGGCATTACAAGAACGCTAGCA 6207 *F flyX_1711713 TTTACATACCATGACGATCCAATTCAAGGAGGTCATACAGGCATTACAAGAACGCTAGCA 6198 *F fly3R_3555248 TTTACATACCATGACGATCCAATTCAAGGAGGTCATACAGGCATTACAAGAACGCTAGCA 6209 *F fly3L_14774061 TTTACATACCATGACGATCCAATTCAAGGAGGTCATACAGGCATTACAAGAACGCTAGCA 5992 *F STALKER2 TTTACATACCATGACGATCCAATTCAAGGAGGTCATACAGGCATTACAAGAACGCTAGCA 5996 *F \************************************************************ *F *F fly3L_18659693 AAAATTAAAAGACATTATTATTGGAAAAATATGACACG-TCATATCAAAGAGTACGTTAA 6266 *F flyX_1711713 AAAATTAAAAGACATTATTATTGGAAAAATATGACACG-TCATATCAAAGAGTACGTTAA 6257 *F fly3R_3555248 AAAATTAAAAGACATTATTATTGGAAAAATATGACACG-TCATATCAAAGAGTACGTTAA 6268 *F fly3L_14774061 AAAATTAAAAGACATTATTATTGGAAAAATATGACACG-TCATATCAAAGAGTACGTTAA 6051 *F STALKER2 AAAATTAAAAGACATTATTATTGGAAAAATATGACACGGTCATATCAAAGAGTACGTTAA 6056 *F \************************************** \********************* *F *F fly3L_18659693 TAAATGTCAAAAATGCCTTACGTCTAAAACAACGACGCATACAAAAACACCCCTTACAAT 6326 *F flyX_1711713 TAAATGTCAAAAATGCCTTACGTCTAAAACAACGACGCATACAAAAACACCCCTTACAAT 6317 *F fly3R_3555248 TAAATGTCAAAAATGCCTTACGTCTAAAACAACGACGCATACAAAAACACCCCTTACAAT 6328 *F fly3L_14774061 TAAATGTCAAAAATGCCTTACGTCTAAAACAACGACGCATACAAAAACACCCCTTACAAT 6111 *F STALKER2 TAAATGTCAAAAATGCCTTACGTCTAAAACAACGACGCATACAAAAACACCCCTTACAAT 6116 *F \************************************************************ *F *F fly3L_18659693 AACGGAAACACCAACTTGCGCTTTCGACAGAGTGATAGTAGACACTATAGGTCCACTACC 6386 *F flyX_1711713 AACGGAAACACCAACTTGCGCTTTCGACAGAGTGATAGTAGACACTATAGGTCCACTACC 6377 *F fly3R_3555248 AACGGAAACACCAACTTGCGCTTTCGACAGAGTGATAGTAGACACTATAGGTCCACTACC 6388 *F fly3L_14774061 AACGGAAACACCAACTTGCGCTTTCGACAGAGTGATAGTAGACACTATAGGTCCACTACC 6171 *F STALKER2 AACGGAAACACCAACTTGCGCTTTCGACAGAGTGATAGTAGACACTATAGGTCCACTACC 6176 *F \************************************************************ *F *F fly3L_18659693 CAAATCGGAAAATGGTAATGAATATGCAGTCACTCTAATCTGTGACTTAACAAAATATTT 6446 *F flyX_1711713 CAAATCGGAAAATGGTAATGAATATGCAGTCACTCTAATCTGTGACTTAACAAAATATTT 6437 *F fly3R_3555248 CAAATCGGAAAATGGTAATGAATATGCAGTCACTCTAATCTGTGACTTAACAAAATATTT 6448 *F fly3L_14774061 CAAATCGGAAAATGGTAATGAATATGCAGTCACTCTAATCTGTGACTTAACAAAATATTT 6231 *F STALKER2 CAAATCGGAAAATGGTAATGAATATGCAGTCACTCTAATCTGTGACTTAACAAAATATTT 6236 *F \************************************************************ *F *F fly3L_18659693 AGTTGCCATACCAATTCCCAACAAAAATGCAAATACAGTCGCAAAAGCTATATTTGAATC 6506 *F flyX_1711713 AGTTGCCATACCAATTCCCAACAAAAATGCAAATACAGTCGCAAAAGCTATATTTGAATC 6497 *F fly3R_3555248 AGTTGCCATACCAATTCCCAACAAAAATGCAAATACAGTCGCAAAAGCTATATTTGAATC 6508 *F fly3L_14774061 AGTTGCCATACCAATTCCCAACAAAAATGCAAATACAGTCGCAAAAGCTATATTTGAATC 6291 *F STALKER2 AGTTGCCATACCAATTCCCAACAAAAATGCAAATACAGTCGCAAAAGCTATATTTGAATC 6296 *F \************************************************************ *F *F fly3L_18659693 ATTCATTCTGAAGTACGGTCCAATGAAGACGTTCATTACGGACATGGGAACAGAATACAA 6566 *F flyX_1711713 ATTCATTCTGAAGTACGGTCCAATGAAGACGTTCATTACGGACATGGGAACAGAATACAA 6557 *F fly3R_3555248 ATTCATTCTGAAGTACGGTCCAATGAAGACGTTCATTACGGACATGGGAACAGAATACAA 6568 *F fly3L_14774061 ATTCATTCTGAAGTACGGTCCAATGAAGACGTTCATTACGGACATGGGAACAGAATACAA 6351 *F STALKER2 ATTCATTCTGAAGTACGGTCCAATGAAGACGTTCATTACGGACATGGGAACAGAATACAA 6356 *F \************************************************************ *F *F fly3L_18659693 GAATAGCATTATAGCCGACTTATGCAAATATCTGAGAATAGACAATATAACGTCTACAGC 6626 *F flyX_1711713 GAATAGCATTATAGCCGACTTATGCAAATATCTGAGAATAGACAATATAACGTCTACAGC 6617 *F fly3R_3555248 GAATAGCATTATAGCCGACTTATGCAAATATCTGAGAATAGACAATATAACGTCTACAGC 6628 *F fly3L_14774061 GAATAGCATTATAGCCGACTTATGCAAATATCTGAGAATAGACAATATAACGTCTACAGC 6411 *F STALKER2 GAATAGCATTATAGCCGACTTATGCAAATATCTGAGAATAGACAATATAACGTCTACAGC 6416 *F \************************************************************ *F *F fly3L_18659693 ACACCACCACCAGACATTAGGTACCGTTGAAAGAAGTCATCGAACCTTCAATGAGTACAT 6686 *F flyX_1711713 ACACCACCACCAGACATTAGGTACCGTTGAAAGAAGTCATCGAACCTTCAATGAGTACAT 6677 *F fly3R_3555248 ACACCACCACCAGACATTAGGTACCGTTGAAAGAAGTCATCGAACCTTCAATGAGTACAT 6688 *F fly3L_14774061 ACACCACCACCAGACATTAGGTACCGTTGAAAGAAGTCATCGAACCTTCAATGAGTACAT 6471 *F STALKER2 ACACCACCACCAGACATTAGGTACCGTTGAAAGAAGTCATCGAACCTTCAATGAGTACAT 6476 *F \************************************************************ *F *F fly3L_18659693 TCGTTCATATATATCAGTAGATAAAACTGACTGGGACATATGGTTACAATATTTTGTGTA 6746 *F flyX_1711713 TCGTTCATATATATCAGTAGATAAAACTGACTGGGACATATGGTTACAATATTTTGTGTA 6737 *F fly3R_3555248 TCGTTCATATATATCAGTAGATAAAACTGACTGGGACATATGGTTACAATATTTTGTGTA 6748 *F fly3L_14774061 TCGTTCATATATATCAGTAGATAAAACTGACTGGGACATATGGTTACAATATTTTGTGTA 6531 *F STALKER2 TCGTTCATATATATCAGTAGATAAAACTGACTGGGACATATGGTTACAATATTTTGTGTA 6536 *F \************************************************************ *F *F fly3L_18659693 CTGTTTTAACACTACACCATCCGTAACGCATAATTACTGTCCATATGAACTAGTATTTGG 6806 *F flyX_1711713 CTGTTTTAACACTACACCATCCGTAACGCATAATTACTGTCCATATGAACTAGTATTTGG 6797 *F fly3R_3555248 CTGTTTTAACACTACACCATCCGTAACGCATAATTACTGTCCATATGAACTAGTATTTGG 6808 *F fly3L_14774061 CTGTTTTAACACTACACCATCCGTAACGCATAATTACTGTCCATATGAACTAGTATTTGG 6591 *F STALKER2 CTGTTTTAACACTACACCATCCGTAACGCATAATTACTGTCCATATGAACTAGTATTTGG 6596 *F \************************************************************ *F *F fly3L_18659693 TAAGACTAGTAATTTAGCAAAACAATTTAGTAACATAGATAATATAGACCCTATATATAA 6866 *F flyX_1711713 TAAGACTAGTAATTTAGCAAAACAATTTAGTAACATAGATAATATAGACCCTATATATAA 6857 *F fly3R_3555248 TAAGACTAGTAATTTAGCAAAACAATTTAGTAACATAGATAATATAGACCCTATATATAA 6868 *F fly3L_14774061 TAAGACTAGTAATTTAGCAAAACAATTTAGTAACATAGATAATATAGACCCTATATATAA 6651 *F STALKER2 TAAGACTAGTAATTTAGCAAAACAATTTAGTAACATAGATAATATAGACCCTATATATAA 6656 *F \************************************************************ *F *F fly3L_18659693 CATAGATGATTACGCTAAAGAAGTTAAATTTAGACTAGAAAACGCGTATAAAAGAGCACG 6926 *F flyX_1711713 CATAGATGATTACGCTAAAGAAGTTAAATTTAGACTAGAAAACGCGTATAAAAGAGCACG 6917 *F fly3R_3555248 CATAGATGATTACGCTAAAGAAGTTAAATTTAGACTAGAAAACGCGTATAAAAGAGCACG 6928 *F fly3L_14774061 CATAGATGATTACGCTAAAGAAGTTAAATTTAGACTAGAAAACGCGTATAAAAGAGCACG 6711 *F STALKER2 CATAGATGATTACGCTAAAGAAGTTAAATTTAGACTAGAAAACGCGTATAAAAGAGCACG 6716 *F \************************************************************ *F *F fly3L_18659693 CATATTATTAGAAAGAAATAAAGTAAAACAGAAAACCAGTTATGATAACAAAGTTAGCGA 6986 *F flyX_1711713 CATATTATTAGAAAGTAATAAAGTAAAACAGAAAACCAGTTATGATAACAAAGTTAGCGA 6977 *F fly3R_3555248 CATATTATTAGAAAGTAATAAAGTAAAACAGAAAACCAGTTATGATAACAAAGTTAGCGA 6988 *F fly3L_14774061 CATATTATTAGAAAGAAATAAAGTAAAACAGAAAACCAGTTATGATAACAAAGTTAGCGA 6771 *F STALKER2 CATATTATTAGAAAGAAATAAAGTAAAACAGAAAACCAGTTATGATAACAAAGTTAGCGA 6776 *F \*************** \******************************************** *F *F fly3L_18659693 TTTTAAACTAAAAGTAGGAGATAACGTTTTAATAAGAAACGAAACAGGTCACAAACTAGA 7046 *F flyX_1711713 TTTTAAACTAAAAGTAGGAGATAACGTTTTAATAAGAAACGAAACAGGTCACAAACTAGA 7037 *F fly3R_3555248 TTTTAAACTAAAAGTAGGAGATAACGTTTTAATAAGAAACGAAACAGGTCACAAACTAGA 7048 *F fly3L_14774061 TTTTAAACTAAAAGTAGGAGATAACGTTTTAATAAGAAACGAAACAGGTCACAAACTAGA 6831 *F STALKER2 TTTTAAACTAAAAGTAGGAGATAACGTTTTAATAAGAAACGAAACAGGTCACAAACTAGA 6836 *F \************************************************************ *F *F fly3L_18659693 ACCAACGTATCTAGGGCCATTCGAAGTAATTAGAATCGAAGAAAACAATAACATAGTAAT 7106 *F flyX_1711713 ACCAACGTATCTAGGGCCATTCGAAGTAATTAGAATCGAAGAAAACAATAACATAGTAAT 7097 *F fly3R_3555248 ACCAACGTATCTAGGGCCATTCGAAGTAATTAGAATCGAAGAAAACAATAACATAGTAAT 7108 *F fly3L_14774061 ACCAACGTATCTAGGGCCATTCGAAGTAATTAGAATCGAAGAAAACAATAACATAGTAAT 6891 *F STALKER2 ACCAACGTATCTAGGGCCATTCGAAGTAATTAGAATCGAAGAAACCAATAACATAGTAAT 6896 *F \******************************************** \*************** *F *F fly3L_18659693 TAGGAATAAAAAGAACAAAGAACAGAAAGTTCATAAGGATAGGTTAAAAATATATAATTA 7166 *F flyX_1711713 TAGGAATAAAAAGAACAAAGAACAGAAAGTTCATAAGGATAGGTTAAAAATATATAATTA 7157 *F fly3R_3555248 TAGGAATAAAAAGAACAAAGAACAGAAAGTTCATAAGGATAGGTTAAAAATATATAATTA 7168 *F fly3L_14774061 TAGGAATAAAAAGAACAAAGAACAGAAAGTTCATAAGGATAGGTTAAAAATATATAATTA 6951 *F STALKER2 TAGGAATAAAAAGACCAAAGACCAGAAAGTTCATAAGGATAGGTTAAAAATATATAATTA 6956 *F \************** \****** \************************************** *F *F fly3L_18659693 ATGAAACGTTTTATACAAAATAAATAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 7226 *F flyX_1711713 ATGAAACGTTTTATACAAAATAAATAACTAAAAAAAAAAAAAAAAAAAAAAA-------G 7210 *F fly3R_3555248 ATGAAACGTTTTATACAAAATAAATAACTAAAAAAAAAAAAAAAAAAAAAAAAAA----G 7224 *F fly3L_14774061 ATGAAACGTTTTATACAAAATAAATAACTAAAAAAAAAAAAAA-G--------------G 6996 *F STALKER2 ATGAAACGTTTTATACAAAATAAATAACTAAAAAAAAAAAAAAAG--------------G 7002 *F \******************************************* \* *F *F fly3L_18659693 GTCTGATCAACCGAAAAAAAAAAAA------TTTAATAAGTTTTTGTCTAAGAAAGTTAA 7280 *F flyX_1711713 GTCTGATCAACCGAAAAAAAAAAAAAAAAAATTTAATAAGTTTTTGTCTAAGAAAGTTAA 7270 *F fly3R_3555248 GTCTGATCAACCGAAAAAAAAAA--------TTTAATAAGTTTTTGTCTAAGAAAGTTAA 7276 *F fly3L_14774061 GTCTGATCAACCGAAAAAAAAAA--------TTTAATAAGTTTTTGTCTAAGAAAGTTAA 7048 *F STALKER2 GTCTGATCAACCGAAAAAAAAAA--------TTTAATAAGTTTTTGTCTAAGAAAGTTAA 7054 *F \*********************** \***************************** *F *F fly3L_18659693 AAATAGAAGCATAACGTAATTATAAACAAC---AAAAAAAAAAATATAAAAAAAAAAAAA 7337 *F flyX_1711713 AAATAGAAGCATAACGTAATTATAAACAAC---AAAAAAAAAAAAATATAAAAAAAAAAA 7327 *F fly3R_3555248 AAATAGAAGCATAACGTAATTATAAACAAC---AAAAAAAAAAA-ATATAAAAAAAAAAA 7332 *F fly3L_14774061 AAATAGAAGCATAACGTAATTATAAACAAC--AAAAAAAAAAAAAATATAAAAAAAAA-- 7104 *F STALKER2 AAATAGAAGCATAACGTAATTATAACCCCCCCAAAAAAAAAAAAAATATAAAAAAAAAAA 7114 *F \************************* \* \* \*********** \*** \********* *F *F fly3L_18659693 A-TTCTTTTACACAAATTATTCTGAGACCAAACTTTTTCTAATAGAAAGGATAAATAAGA 7396 *F flyX_1711713 --TTCTTTTACACAAATTATTCTGAGACCAAACTTTTTCTAATAGAAAGGATAAATAAGA 7385 *F fly3R_3555248 A-TTCTTTTACACAAATTATTCTGAGACCAAACTTTTTCTAATAGAAAGGATAAATAAGA 7391 *F fly3L_14774061 --TTCTTTTACACAAATTATTCTGAGACCAAACTTTTTCTAATAGAAAGGATAAATAAGA 7162 *F STALKER2 AATTCTTTTACACAAATTATTCTGAGACCAAACTTTTTCTAATAGAAAGGATAAATAAGA 7174 *F \********************************************************** *F *F fly3L_18659693 AATAATATAAGAAAAAAAA--TGTTTTAATTAATTAAATGACTACATATATTACGTCATT 7454 *F flyX_1711713 AATAATATAAGAAAAAAAA--TGTTTTAATTAATTAAATGACTACATATATTACGTCATT 7443 *F fly3R_3555248 AATAATATAAGAAAAAAAAAATGTTTTAATTAATTAAATGACTACATATATTACGTCATT 7451 *F fly3L_14774061 AATAATATAAGAAAAAAAA--TGTTTTAATTAATTAAATGACTACATATATTACGTCATT 7220 *F STALKER2 AATAATATAAGAAAAAAAA--TGTTTTAATTAATTAAATGACTACATATATTACGTCATT 7232 *F \******************* \*************************************** *F *F fly3L_18659693 TCTCTAAAAAGGGAGGTGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATAT 7514 *F flyX_1711713 TCTCTAAAAAGGGAGGTGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATAT 7503 *F fly3R_3555248 TCTCTAAAAAGGGAGGTGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATAT 7511 *F fly3L_14774061 TCTCTAAAAAGGGAGGTGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATAT 7280 *F STALKER2 TCTCTAAAAAGGGAGGTGTAGTGTATCTACCCTCACTATAACTCTACTCTACATATATAT 7292 *F \************************************************************ *F *F fly3L_18659693 AAGTAACGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCA 7574 *F flyX_1711713 AAGTAACGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCA 7563 *F fly3R_3555248 AAGTAACGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCA 7571 *F fly3L_14774061 AAGTAACGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCA 7340 *F STALKER2 AAGTAACGTACATACATTGTGACACTTTGTTGCAAACACAAATAAACATAATTCACATCA 7352 *F \************************************************************ *F *F fly3L_18659693 AAGACCACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACA 7634 *F flyX_1711713 AAGACCACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACA 7623 *F fly3R_3555248 AAGACCACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACA 7631 *F fly3L_14774061 AAGACCACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACA 7400 *F STALKER2 AAGACCACATGCACTTACATAAACACTCCAGCCAATGAAATACGATCTAACGCTTATACA 7412 *F \************************************************************ *F *F fly3L_18659693 TAAGCCGATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGC 7694 *F flyX_1711713 TAAGCCGATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGC 7683 *F fly3R_3555248 TAAGCCGATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGC 7691 *F fly3L_14774061 TAAGCCGATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGC 7460 *F STALKER2 TAAGCCGATCGCGGAGCGTGAGAATGCTGAGCATGCACTTAGCAGCTCAAGTGGTCAAGC 7472 *F \************************************************************ *F *F fly3L_18659693 CATACATAACATATGTATGCCTTCTGCATACACATGTATATGTATATACA---------A 7745 *F flyX_1711713 CATACATAACATATGTATGCCTTCTGCATACACATGTATATGTATATACA---------A 7734 *F fly3R_3555248 CATACATAACATATGTATGCCTTCTGCATACACATGTATATGTATATACA---------A 7742 *F fly3L_14774061 CATACATAACATATGTATGCCTTCTGCATACACATGTATATGTATATACAATATATACAA 7520 *F STALKER2 CATACATAACATATGTATGCCTTCTGCATACACATGTATATGTATATACA---------A 7523 *F \************************************************** \* *F *F fly3L_18659693 TATGTACAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGG 7805 *F flyX_1711713 TATGTACAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGA 7794 *F fly3R_3555248 TATGTACAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGA 7802 *F fly3L_14774061 TATGTACAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGG 7580 *F STALKER2 TATGTACAATATGTAAGAACACCATGTACGGGTAGCTGTACCCAAAGACAGCAACATAGG 7583 *F \*********************************************************** *F *F fly3L_18659693 ATTTCATTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATT 7865 *F flyX_1711713 ATT-CATTCAAATAAAACGATTCAAACGAAACAGACGCTCTGAGCTATTCAATATCTATT 7853 *F fly3R_3555248 ATT-CATTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATT 7861 *F fly3L_14774061 ATT-CATTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATT 7639 *F STALKER2 ATT-CATTCAAATAAAACGATTCAAACGGAACAGACGCTCTGAGCTATTCAATATCTATT 7642 *F \*** \************************ \******************************* *F *F fly3L_18659693 ACACTGAGCTATTACTTATTACTTATTACA---------- 7895 *F flyX_1711713 ACACTGAGCTATTACTTATTACTTATTACA---------- 7883 *F fly3R_3555248 ACACTGAGCTATTACTTATTACTTATTACAAAATTAAATA 7901 *F fly3L_14774061 ACACTGAGCTATTACTTATTACTTATTACA---------- 7669 *F STALKER2 ACACTGAGCTATTACTTATTACTTATTACA---------- 7672 *F \****************************** # *U FBrf0178737 *a Gelbart *b W.M. *t 2004.7.17 *T personal communication to FlyBase *u *F From: William Gelbart *F Date: Sat, 17 Jul 2004 23:10:09 -0400 (EDT) *F To: brian@morgan.harvard.edu, pavel@morgan.harvard.edu, peili@morgan.harvard.edu *F Subject: my look at Peili's BLAST data *F Cc: gelbart@morgan.harvard.edu *F *F Folks, *F *F I looked at Peili's BLASTn of the Dpse GenBank accessions. I ended *F up with 122 unambiguous hits (a couple of these rows are to the same *F gene because they were inadvertantly present in two accessions). The *F following table is of these 122 good hits. The table (tab-delimited *F format) has 5 columns: *F 1 - Dpse\Gene *F 2 - GB_accession (where there were multiple genes per record, the coordinates *F are shown as well as orientation) *F 3 - Dpse Contig-1 (the correct hit, except in 3 cases where the gene *F spans two contigs). *F 4 - Dpse Contig-2 (in 3 cases, is also a correct hit; in a few other *F cases, weaker hits are noted here) *F 5 - Comments *F *F I'll send the list of the other 16 problematic cases separately. *F *F Bill *F *F ============================================================================= *F *F Dpse\28SrRNA AF059868 Contig334: 32-168 (comp) 0 0 *F Dpse\5SrRNA U58691 Contig7754: 1264-1543 (comp) Several weaker hits as well. Divergent copies may be found in other contigs. *F Dpse\Adam AY190942 : <3689..>4979 Contig174: 18800-20090 (comp) 0 0 *F Dpse\ade3 X06285 : 468..1831 Contig1755: 11810-13177 (comp) 0 0 *F Dpse\Adh M60979 Contig1559: 9763-13248 (comp) 0 0 *F Dpse\Adhr U64535 Contig1559: 9763-13248 (comp) Lots of weaker hits 0 *F Dpse\Ama X77710 Contig1677: 67897-67995 0 0 *F Dpse\amd AF293722 Contig2327: 1814-3284 (comp) 0 0 *F Dpse\Amy2 U20332 Contig20: 38424-41018 (dir) 0 0 *F Dpse\Amy3 X76242 Contig20: 38433-40846 (dir) 0 0 *F Dpse\Amyrel U82556 Contig1864: 12047-14439 (comp) 0 0 *F Dpse\anon-Est-5 AF196011 Contig30: 4294-4819 (comp) 0 0 *F Dpse\anon-msRNA AF510848 Contig4576: 31231-31330 (comp) 0 0 *F Dpse\Antp AY190944 : <8728..>8979 Contig2736: 925-1178 (dir) 0 0 *F Dpse\ap AY190945 : <21777..>35693 Contig8262: 44626-53046 (dir) 0 0 *F Dpse\Aprt L06281 Contig5156: 23373-24080 (comp) 0 0 *F Dpse\Arc42 AY190960 : <13315..>14677 Contig5649: 67210-68572 (dir) 0 0 *F Dpse\bcd AF320167 Contig1677: 62184-62241 (comp) 0 short sequence (60bp); hit is 58/58 *F Dpse\CG11915 AY190946 : <41130..>43232 Contig3421: 28628-30730 (dir) 0 0 *F Dpse\CG12130 AY190942 : <108..>1293 Contig174: 22496-23692 (comp) 0 0 *F Dpse\CG12134 AY190942 : <5828..>7167 Contig174: 16615-17954 (comp) 0 0 *F Dpse\CG13030 AY190946 : <1..>229 Contig2223: 17224-17452 (dir) 0 0 *F Dpse\CG13309 AY452758 Cont4117: 6267-6629 (dir) 0 0 *F Dpse\CG1418 AY190942 : complement(<1722..>2408) Contig174: 21381-22067 (comp) 0 0 *F Dpse\CG14291 AY190947 : complement(<41655..>42336) Contig3752: 6142-6819 (comp) 0 0 *F Dpse\CG14292 AY190947 : complement(<34040..>34618) Contig3752: 13877-14461 (comp) 0 0 *F Dpse\CG14297 AY190947 : <28347..>29260 Contig3752: 19227-20134 (comp) 0 0 *F Dpse\CG14298 AY190947 : <7719..>8620 Contig3752: 40725-39825 (comp) 0 0 *F Dpse\CG15605 AF476111 : complement(<9851..>10342) Contig1765: 5944-6435 (comp) 0 0 *F Dpse\CG15611 AF476111 : <1..225 Contig1765: 16140-16358 (comp) 0 0 *F Dpse\CG15918 AF476111 : <12341..>12592 Contig1765: 3694-3855 (comp) 0 0 *F Dpse\CG17186 AY190960 : complement(<11997..>13047 Contig5649: 56891-66941 (dir) 0 0 *F Dpse\CG2206 AY452761 Contig3439: 20160-20474 (comp) 0 0 *F Dpse\CG30421 AY190942 : <25091..>37693 Contig5077: 10867-23228 (comp) 0 0 *F Dpse\CG32170 AY190946 : <16582..>18321 Contig3421: 3971-5712 (dir) 0 0 *F Dpse\CG32171 AY190946 : <6132..>37921 Contig2223: 23262-29365 (dir) = AY190946: 6132-12221 Contig3421: 1-25392 (dir) = AY190946: 12599-37921 CG32171 spans both Contigs *F Dpse\CG3880 AY190942 : <43749..>44081 Contig5077: 4466-5798 (comp) 0 0 *F Dpse\CG4390 AY190949 : complement(<11296..>12162) Contig6561: 8567-9432 (comp) 0 0 *F Dpse\CG4562 AY190960 : complement(<3900..>10687) Contig5649: 57789-54583 (dir) 0 0 *F Dpse\CG4572 AY190960 : complement(<1..>1302) Contig5649: 53880-55181 (dir) 0 0 *F Dpse\CG4686 AY190960 : <1714..>3097 Contig5649: 55593-56973 (dir) 0 0 *F Dpse\CG4973 AY190949 : <14193..>15393 Contig6561: 6556-7812 (comp) 0 0 *F Dpse\CG5245 AY190960 : <15209..>16774 Contig5649: 69104-70676 (dir) 0 0 *F Dpse\CG9196 AY190942 : <40664..>42391 Contig5077: 6155-7082 (comp) 0 0 *F Dpse\CG9895 AY190948 : complement(<5449..>7109) Contig3953: 53105-54665 (dir) 0 0 *F Dpse\CG9951 AY190946 : <1575..>3236 Contig2223: 18798-20459 (dir) 0 0 *F Dpse\cid AF435467 Contig3143: 253996-254769 (comp) 0 0 *F Dpse\Cyp1 AF025803 Contig4378: 1689-3468 (dir) 0 0 *F Dpse\Dfd X77719 Contig1677: 100883-100979 (comp) 0 0 *F Dpse\dos AY128672 Contig4096: 41838-47942 (comp) many weaker hits 0 *F Dpse\dpp U63856 Contig4388: 47458-54785 (dir) 0 0 *F Dpse\Eip74EF S73515 Contig625: 5179-5904 (comp) = S73515 728-1 Contig5526: 9831-14799 (dir) = S73515 728-2274 Eip74EF spans both Contigs *F Dpse\en AF476326 Contig3161: 12018-12394 (dir) 0 0 *F Dpse\endoA AY190947 : <37296..>38405 Contig3752: 10096-11205 (comp) 0 0 *F Dpse\Eno AF025805 Contig1667: 27663-28970 (comp) 0 0 *F Dpse\Est-5A AF016135 Contig30: 798-1437 (comp) 0 0 *F Dpse\Est-5B M55907 : 2735-4427 Contig30: 2463-4155 (comp) 0 0 *F Dpse\Est-5C AF016143 Contig30: 4570-6889 (comp) 0 0 *F Dpse\Est-5C M55907 : 230-1927 Contig30: 4962-6359 (comp) 0 0 *F Dpse\Est-5C/Est-5B-intergenic-spacer AF485508 Contig30: 4309-4807 (comp) 0 0 *F Dpse\eve AF476426 Contig174: 8305-8768 (comp) 0 0 *F Dpse\eve AY190942 : <14370..>15528 Contig174: 7559-8708 (comp) 0 0 *F Dpse\exu1 AF476528 Contig3698: 120231-120587 (dir) 0 0 *F Dpse\exu2 AY337547 Cont766: 19105-20135 (dir) 0 0 *F Dpse\ey AF451009 Contig6074: 5817-7425 (comp) 0 0 *F Dpse\fru AF297059 Contig1814: 66678-67357 (comp) 0 0 *F Dpse\ftz AY190944 : complement(<40669..>42162 Contig2736: 32852-34356 (dir) 0 0 *F Dpse\Gad1 AF025807 Contig3525: 12462-18500 (dir) 0 0 *F Dpse\Gapdh2 AF025809 Contig6461: 577-1484 (comp) 0 0 *F Dpse\Gld M29299 Contig268: 151942-164358 (dir) 0 0 *F Dpse\hb AF461311 Contig2373: 42031-42534 (dir) 0 0 *F Dpse\His3 AB044538 Contig138: 1047-1285 (dir) 0 Maybe a bit of Histone cluster *F Dpse\Hsp83 AF006529 Contig3978: 5279-7282 (dir) 0 0 *F Dpse\Indy-2 AY190949 : <22..>1971 Contig6561: 18791-20746 (comp) 0 0 *F Dpse\janA S77099 : 180..749 Contig4458: 22796-23363 (dir) 0 0 *F Dpse\janB S77099 : 927..1539 Contig4458: 23543-24158 (dir) 0 0 *F Dpse\l(2)gl X73259 Contig2108: 607-9611 (dir) 0 0 *F Dpse\l(3)87Df M33977 Contig1889: 2420-8935 (dir) 0 0 *F Dpse\lab X77713 Contig1676: 53127-53221 (comp) 0 0 *F Dpse\Mef2 AF476728 Contig4878: 16175-16559 (dir) 0 0 *F Dpse\mit(1)15 U54997 Contig1569: 11426-13007 (comp) 0 0 *F Dpse\Mlc1 L08052 Contig2263: 25784-28654 (comp) 0 0 *F Dpse\mt:CoI AF050745 Contig3756: 295-789 (dir) 0 0 *F Dpse\mt:CoII AF519348 Contig3756: 892-1578 (dir) 0 0 *F Dpse\mt:CoIII AF519380 Contig3756: 3237-3653 (dir) 0 0 *F Dpse\mt:lrRNA M93993 Contig4895: 1-471 (comp) 0 0 *F Dpse\mt:ND4 AF451105 : <1..111 Contig7113: 1915-2025 (dir) 0 0 *F Dpse\mt:ND5 AF451105 : 178..>997 Cont7113: 2062-2237 (dir) = AF451105 178-352 Cont4629: 1144-1724 (comp) = AF451105 406-986 mt:ND5 spans both Contigs. *F Dpse\mt:tRNA:H AF451105 : 112..177 Cont7113: 2026-2062 0 0 *F Dpse\ninaE AF450965 Contig5649: 78919-80361 (comp) 0 0 *F Dpse\ninaE AY190960 : complement(<24884..>26570) Contig5649: 78818-80506 (dir) 0 0 *F Dpse\Obp83cd AJ618970 Contig2923: 7221-14869 (comp) 0 0 *F Dpse\pb S77929 Contig1677: 43021-43827 (comp) 0 0 *F Dpse\Pcp X06285 : complement(1831..2681) Contig1755: 10960-11810 (comp) 0 0 *F Dpse\per L81275 Contig4134: 24441-25945 (comp) 0 0 *F Dpse\pim AY352648 Contig4930: 1855-3774 (comp) 0 0 *F Dpse\Pk92B AY190960 : <18106..>23153 Contig5649: 72008-77059 (dir) 0 0 *F Dpse\Rh2 AY190947 : <29475..>30808 Contig3752: 17677-19012 (comp) 0 0 *F Dpse\Rh3 AY190949 : complement(<38022..>39170) Contig3109: 15131-16279 (dir) 0 0 *F Dpse\Rh4 AY190946 : complement(<458..>1048) Contig2223: 17681-18263 (dir) 0 0 *F Dpse\RpII215 AJ544770 Cont4918: 11604-12613 (dir) 0 0 *F Dpse\RpL32 S59382 Contig4458: 15205-17323 (dir) 0 0 *F Dpse\run AF196134 Contig2329: 114835-115254 (comp) 0 0 *F Dpse\ry AF543163 Contig1889: 5288-6853 (dir) 0 0 *F Dpse\Scr X77717 Contig2736: 56913-57010 (dir) 0 0 *F Dpse\sesB AF025798 Contig2365: 40791-41819 (comp) 0 0 *F Dpse\sisA AF045586 Contig446: 21271 -23761 (dir) 0 0 *F Dpse\small-wing AF543828 Contig2592: 7714-13202 (comp) 0 0 *F Dpse\Sod AF059882 Contig2718: 11393-11674 (comp) 0 0 *F Dpse\swa AF169634 Contig4342: 27792-30224 (dir) 0 0 *F Dpse\thr AY352649 Contig1765: 50020-54674 (dir) 0 0 *F Dpse\Tl L25390 Contig148: 75412-79099 (comp) 0 0 *F Dpse\Tm1 AF039273 Contig248: 14444 -14873 (dir) 0 0 *F Dpse\Tpi AF025814 Contig3266: 11667-12432 (comp) 0 0 *F Dpse\Trap100 AY208899 Contig4576: 30752-33036 (dir) 0 0 *F Dpse\twi AY190948 : <42122..>43025 Contig3211: 99875-98995 (dir) 0 0 *F Dpse\Ubx U03179 Contig1892: 56716-179803 (comp) 0 0 *F Dpse\Uro X57113 Contig1794: 70672-72897 (comp) 0 0 *F Dpse\Vha14 AF025796 Contig3108: 31103-31372 (dir) 0 0 *F Dpse\vsg AY452754 Contig5648: 620-985 (comp) 0 0 *F Dpse\y AJ300666 Contig3656: 19883-25140 (dir) 0 0 *F Dpse\zen L17338 Contig1677: 59784-60419 (comp) 0 0 # *U FBrf0178738 *a Gelbart *b W.M. *t 2004.7.17 *T personal communication to FlyBase *u *F From: William Gelbart *F Date: Sat, 17 Jul 2004 23:11:30 -0400 (EDT) *F To: brian@morgan.harvard.edu, pavel@morgan.harvard.edu, peili@morgan.harvard.edu *F Subject: the 16 problematic cases *F Cc: gelbart@morgan.harvard.edu *F *F Here are the problematic cases. The columns are the same. *F *F Bill *F *F ============================================================================ *F *F Dpse\Amy1 AF476112 Contig20: 43001-43423 (comp) 0 In Contig20, 2 additional & better copies of bp1-114 on opposite strand): 34125-34238 (dir) and 40566-40679 (dir) *F Dpse\bb Z28411 X X No hits. *F Dpse\CG31155 AY190960 : <45653..>45871 Contig7464: 10198-10326 (dir) Contig7462: some possible duplicate assemblies between Contigs *F Dpse\CG4538 AY190960 : complement(<40232..>43546) Contig7464: 4774-8091 (dir) Contig7462: some possible duplicate assemblies between Contigs *F Dpse\CG4733 AY190960 : <32471..>38710 Contig5649: 84718-87423 (dir) Contig7464: some possible duplicate assembly here w/i Contig5649 & between Contigs. *F Dpse\EcR AF476222 Contig591: 2016-2353 (comp) Contig589: 4121-4456 (comp) Close call: Contig591 = 336/338 (no gaps); Contig589 = 335/338 (1 gap) *F Dpse\Gpdh L41249 X X No hit. *F Dpse\His2A AJ224812 X X Accession really intergenic spacer between His2A & His2B *F Dpse\His2B AJ224812 X X Accession really intergenic spacer between His2A & His2B *F Dpse\mt:ND1 M93993 X X No such annotation in GenBank record *F Dpse\mt:srRNA AF220074 X X No real hit. *F Dpse\mt:tRNA:L:CUN M93993 X X No such annotation in GenBank record *F Dpse\R1-element\ORF U23201 X X ignore - transposon *F Dpse\R2-element\ORF U64955 X X ignore - transposon *F Dpse\Surf6 AY190960 : complement(<44414..>45452) Contig7464: 8658-9987 (dir) Contig7462: some possible duplicate assemblies between Contigs *F Dpse\vg AF476818 Contig2562: 83356-83871 (comp) Contig3145: 1-326 (comp) Contig2562 & Contig3145 both look like primary hits -- duplicate assembly? # *U FBrf0178739 *a Chen *b E. *t 2004.3.1 *T personal communication to FlyBase *u *F Date: Fri, 27 Feb 2004 15:55:23 \-0600 *F To: cy200@gen.cam.ac.uk *F From: Elizabeth Chen *F Dear Chihiro, *F Eric forwarded your query to me and I'd be happy to answer your question. *F The MHC-tauGFP construct was made by the following steps: *F (1) Linearize the BscMHCRV vector (from Corey Goodman's lab) with RV. *F This vector contains ~2.5kb regulatory sequence of MHC, upstream of *F the translational start site (including the 1st and part of the 2nd *F exons). *F (2) Digest the pBD1128 plasmid (from Corey Goodman lab) with EcoRI *F and XbaI to get tau-GFPS65T, and clone it into the RV site of *F BscMHCRV to make BscMHC-tauGFP. *F (3) Digest BscMHC-tauGFP with XhoI and XbaI, and clone the MHC-tauGFP *F fragment into the pCaSpeR4 vector for P-element mediated germline *F transformation. *F Hope this helps. *F Best wishes, *F Elizabeth *F >> To: eolson@hamon.swmed.edu *F >> Subject: FlyBase Query (cy2125) *F >> Cc: cy200@gen.cam.ac.uk *F >> From: Chihiro Yamada *F >> Date: Mon, 8 Dec 2003 14:06:23 \+0000 *F >> *F >> Dear Dr Olsen. *F >> *F >> I am currently curating your paper for FlyBase: *F >> *F >> Chen et al., 2003, Cell 114(6):751--762 *F >> *F >> I have a quick question I was hoping you could answer for me. *F >> *F >> MHC-tauGFP *F >> ========== *F >> You use a MHC-tauGFP line in your paper. We don't have this construct *F >> in our records. Can you give me some molecular details about it? *F >> *F >> Any new data you send me will be curateed as a personal communication *F >> to FlyBase. *F >> *F >> Best wishes, *F >> *F >> Chihiro *F \-- *F Elizabeth Chen *F Department of Molecular Biology, NA8.510 *F UT Southwestern Medical Center at Dallas *F 6000 Harry Hines Blvd. *F Dallas, Texas 75390-9148 *F phone: 214-648-1189 *F fax: 214-648-1196 *F email: chen@hamon.swmed.edu # *U FBrf0178740 *a Hartenstein *b V. *t 2004 *T personal communication to FlyBase *u *F We speak of organs or tissues at a stage when these cells have reached 'a *F significant state' of differentiation. This means that 'a significant number' *F of the molecules required for the function of these cells (e.g., channels or *F neurotransmitters in case of nerve cells) is expressed. Such a state is *F reached for most structures in Drosophila during stage 16 (70% of development). *F Before this stage we speak of primordia, anlagen, anlagen in statu nascendi. *F These terms are defined *F \-(i) in regard to the structure they give rise to *F \-(ii) in regard to their location *F (i)example: the anlage of the central brain consists of the cells (of the *F stage 5-7 embryo) which give rise to the central brain; see definition of *F central brain *F (ii)example: the anlage of the central brain is located between 80 and 90% egg *F length and 30% and 90% egg width. This is obviously very imprecise; the *F location of anlagen and primordial should be given graphically. *F Primordium *F The times at which we start speaking of primordium vs organ, or anlage vs *F primordium, are very difficult to pin down precisely. At a given stage, we *F might be able to pinpoint one boundary of a primordium (e.g., the boundary *F that separates it from the dorsally adjacent primordium), but not another *F boundary (e.g. the boundary that separates it from the anteriorly adjacent *F primordium). Therefore, definitions have a lot of slack; developmental biology *F is not yet mathematics. *F Different types of primordial: If all boundaries of a primordium are defined, *F and there is na 1:1 relationship of the primordium to the organ it gives rise *F to, we might call that a 'distinct' or 'specific' primordium (P1). *F Frequently, the primordia are cell populations that include more than one *F later cell type/organ in them. We might call that a 'complex primordium' (P2). *F In cases where primordia are incomplete at a given stage (we can pinpoint a *F structurally visible condensation or outgrowth, but it does not yet contain *F all the cells it will later have), we might call this 'primordium in statu *F nascendi' (P3). *F These terms may eventually prove useful for denoting details of organogenesis, *F but should not be incorporated in the controlled vocabulary of the in situ *F database. *F Anlage *F Anlagen are populations of contiguous cells, typically arranged in one plane, *F that are morphologically indistinct, but that already correspond in extent to *F a later organ/tissue. Anlagen of different organs can (or should) be defined *F at different stages. In theory, one could map each part of the mature organism *F on the blastoderm. For purposes of developmental analysis, that may not be too *F useful. For example, one could, very imprecisely, define where an organ like *F the trachea maps at the blastoderm stage. Most likely, cells of this *F 'blastoderm tracheal anlage' woulod not be contiguous, but intermikngled with *F cells that form dorsal epidermis or sensory organs. More importantly, they are *F (according to our current knowledge) no genes involved in tracheal *F specification that are expressed already at the blastoderm stage. The earliest *F tracheal genes, such as trachealess, come on around stage 9 in segmental *F domains that reflect the tracheal primordial which can be seen slightly later. *F Therefore, in the controlled vocabulary, the term 'tracheal anlage' appears *F for the stage 9/10 embryo. *F Different types of anlagen: The same distinctions drawn out for primordial *F above can be stated for anlagen. We may distinguish 'discrete' or 'specific' *F anlagen which contain progenitors of only one organ from 'complex' or *F 'inclusive' anlagen that give rise to more than one structure. 'anlagen in *F statu nascendi' are domains that do not yet coincide 1:1 with a later organ. *F Anlagen in statu nascendi are typically defined for the early blastoderm by *F the expression domains of genes which, in the late blastoderm or later, are *F expressed in specific anlagen, but initially come on in larger domains. *F Germ layers *F Germ layers are 'super complex primordia'. They arise during gastrulation and *F later split up into discrete organ primordia. There is a lot of slack in the *F nomenclature in regard to terms that refer to germ layers and to organs. For *F example, we speak of ventral ectoderm or the 'primordium of the ventral *F epidermis'. Or of 'anterior endoderm' or 'anterior midgut primordium'. # *U FBrf0178741 *a Beitel *b G. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Wed, 25 Feb 2004 14:15:55 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: Gene data (problem or question) *F comments: The predicted gene structure and protein sequence in release 3.1 *F for Sinuous (CG10624) is based on an aberrantly spliced cDNA which has a novel *F and probably irrelevant c-Terminal sequence. The correct structure and *F sequence for the sinuous gene product is given by the representative cDNA with *F accession number AY423544. See Schulte,J., Hirschi,A., Tepass,U. and *F Beitel,G.J. ' Sinuous is a Drosophila claudin required for septate junction *F organization and epithelial tube size control' J. Cell Biol. 164 (2), 313-323 *F (2004) for details. Look in the materials and methods. We go through a lot of *F details of how we determined the correct structure of this gene. *F Greg Beitel *F realname: Greg Beitel *F reply-to: beitel@northwestern.edu # *U FBrf0178742 *a Overton *b P. *t 2003.2.19 *T personal communication to FlyBase *u *F From: 'Paul Overton' *F To: ''Gillian Millburn \(Genetics\)'' *F Subject: Another merge *F Date: Wed, 19 Feb 2003 12:42:07 \-0000 *F Hi Gillian. *F Another merge for you I think. *F So EB1 is described in Allada 98 as being in an intron of clk in 66A, *F and having homology to mammalian EB1, however it's not associated with *F an ORF on the sequence. *F CG32371 is sitting in an intron of clk, and has homology to mammalian *F EB1. *F What d'you reckon? *F I am a little unsure about the name EB1, as there is a previously *F published Eb1 in 42 \- do you normally allow this sort of thing? *F P # *U FBrf0178743 *a Ashburner *b M. *t 2004.3.31 *T personal communication to FlyBase *u *F Date: Wed, 31 Mar 2004 10:51:18 \+0100 (BST) *F From: 'Michael Ashburner (Genetics)' *F Subject: Personal communication from M. Ashburner *F To: gm119@gen.cam.ac.uk *F Cc: ma11@gen.cam.ac.uk, sws4@psu.edu *F Gillian *F At the Fly meeting Steve Schaeffer pointed out to me that the melanogaster *F 'gene' Jonah99 is probably from pseudoobscura, being a contaminant clone. *F I confirm this. The sequence (AF315732) has no significant hit to the *F melanogaster nucleotides (BLAST at Indiana) but has a very good hit to *F pseudoobscura at Baylor: *F >Contig1160_Contig3376 Length = 2263003 *F Score = 2819 bits (1422), Expect = 0.0 *F Identities = 1597/1662 (96%), Gaps = 14/1662 (0%) *F Strand = Plus / Plus *F and to several other contigs: *F Contig1160_Contig3376 2819 0.0 *F Contig7471_Contig6056 860 0.0 *F Contig3531_Contig5436 785 0.0 *F Contig1018_Contig1081 785 0.0 *F Contig3342_Contig5418 775 0.0 *F Contig8255_Contig717 761 0.0 *F Contig6581_Contig6381 761 0.0 *F Contig922_Contig1659 741 0.0 *F Contig7486_Contig7520 733 0.0 *F Contig2230_Contig3351 731 0.0 *F Contig2556_Contig5224 714 0.0 *F Contig3295_Contig8282 702 0.0 *F Contig446_Contig3289 698 0.0 *F Contig815_Contig5737 696 0.0 *F Contig6558_Contig6475 696 0.0 *F Contig7446_Contig2444 694 0.0 *F Contig3479_Contig7346 690 0.0 *F Contig2561_Contig6127 686 0.0 *F Contig1741_Contig5707 670 0.0 *F Contig4374_Contig4847 668 0.0 *F Contig2105_Contig7229 666 0.0 *F Contig6586_Contig7863 654 0.0 *F Contig7487_Contig8004 652 0.0 *F Contig152_Contig617 644 0.0 *F Contig6654_Contig5965 642 0.0 *F Goodness knows what this is. The Jonah genes were isolated as midgut expressors *F by Mike Akam and John Carlson. Later Janet Collett isolated this clone and *F reckoned it corresponded to Jonah99. There was no real evidence for this. *F .. *F Michael # *U FBrf0178744 *a Goldstein *b E.S. *t 2004.3.15 *T personal communication to FlyBase *u *F To: e.goldstein@asu.edu *F Subject: FlyBase query *F Cc: gm119@gen.cam.ac.uk *F From: Gillian Millburn (Genetics) *F Date: Tue, 21 Oct 2003 11:47:26 \+0100 *F Dear Dr. Goldstein, *F I am curating your paper for FlyBase: *F Goldstein et al., 2001, Molec. Genet. Genomics 266(4): 695--700 *F and I have a few questions. Sorry it has taken us so long to get to *F your paper, we got a little behind with MGG. *F .. *F The names of the alleles given in Table 1. of your MGG paper are *F very similar to those in Figure 9. of: *F Currie and Sullivan, 1994, J. Biol. Chem. 269(40): 24679--24687 *F and in that JBC paper they reference your 1993 ADRC abstract: *F Goldstein et al., 1993, A. Dros. Res. Conf. 34: 301 *F 'Further studies on the Drosophila homolog of the Jun oncogene.' *F Here is a Table listing the symbols in the Currie and Sullivan paper *F and the allele from Table 1 of your MGG paper that I think the Currie *F and Sullivan alleles correspond to: *F | Currie and | allele from Table 1 | *F | Sullivan | of your MGG paper | *F | | (complementation group| *F | | in brackets) | *F \------------------------------------------ *F Fig 9A| 1.2 | 1-2 (A) *F | 1.11 | not in Table 1 *F | 5.2 | 5-2 (G) *F | 5.3 | 5-3 (E) *F | 5.11 | 5-11 (K) *F | 6.6 | 6-6 (B) *F | 7.12 | 7-12 (I) *F | 10.5 | 10-5 (B or F ..) *F | 10.9 | 10-9 (F) *F | 12.6 | 12-6 (D) *F | 14.10 | 14-10 (F) *F | 14.39 | 14-39 (L) *F | 15.4 | 15-4 (B) *F | 17.5 | 17-5 (M) *F | 19.22 | 19-22 (N) *F | 20.10 | 20-10 (O) *F | 20.35 | 20-35 (F) *F | 21.15 | 21-15 (J) *F | 22.21 | 22-21 (C) *F | 22.26 | 22-26 (I) *F | 24.15 | 24-15 (P) *F | 28.19 | not in Table 1 *F | 29.26 | 29-26 (R) *F | x-1 | Df(2R)X1 *F \------------------------------------------ *F Fig9B | 8.1 | not in Table 1 *F | 8.4 | 8-4 (B) *F | 9.24 | 9-24 (B) *F | 14.35 | 14-35 (B) *F | 19.51 | 19-51 (B) *F | 21.25 | not in Table 1 *F | 21.30 | not in Table 1 *F | 29.42 | 29-42 (B) *F | 80.30 | not in Table 1 *F | 83.21 | not in Table 1 *F \------------------------------------------ *F The difference seems to be that Currie and Sullivan used a '.' where *F you used a '-'. I suspect that this means that the complementation *F groups in Figure 9. of the Currie and Sullivan paper do correspond to *F alleles listed in Table 1. of your paper, which means I could merge *F several gene records in FlyBase. However, I wanted to make sure that my *F hunch was correct before I do this. Do you have any information that *F might help me confirm that the complementation groups given in Figure *F 9. of the Currie and Sullivan, 1994 paper are the same as in Table 1. *F of your MGG paper, for example, did you give them these particular *F stocks ? *F .. *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Mon, 15 Mar 2004 13:30:57 \-0700 *F From: Elliott Goldstein *F Subject: Re: FlyBase query *F To: 'Gillian Millburn (Genetics)' *F sorry for taking so long in getting back to you, this has been a busy *F semester. if any of this is still unclear, let me know. *F elliott goldstein *F Gillian Millburn (Genetics) wrote: *F >Dear Dr. Goldstein, *F > *F >I am curating your paper for FlyBase: *F > *F >Goldstein et al., 2001, Molec. Genet. Genomics 266(4): 695--700 *F > *F >and I have a few questions. Sorry it has taken us so long to get to *F >your paper, we got a little behind with MGG. *F > *F .. *F > The names of the alleles given in Table 1. of your MGG paper are *F >very similar to those in Figure 9. of: *F > *F >Currie and Sullivan, 1994, J. Biol. Chem. 269(40): 24679--24687 *F > *F >and in that JBC paper they reference your 1993 ADRC abstract: *F > *F >Goldstein et al., 1993, A. Dros. Res. Conf. 34: 301 *F >'Further studies on the Drosophila homolog of the Jun oncogene.' *F > *F >Here is a Table listing the symbols in the Currie and Sullivan paper *F >and the allele from Table 1 of your MGG paper that I think the Currie *F >and Sullivan alleles correspond to: *F > *F > *F > | Currie and | allele from Table 1 | *F > | Sullivan | of your MGG paper | *F > | | (complementation group| *F > | | in brackets) | *F >------------------------------------------ *F >Fig 9A| 1.2 | 1-2 (A) *F > | 1.11 | not in Table 1 *F > | 5.2 | 5-2 (G) *F > | 5.3 | 5-3 (E) *F > | 5.11 | 5-11 (K) *F > | 6.6 | 6-6 (B) *F > | 7.12 | 7-12 (I) *F > | 10.5 | 10-5 (B or F ..) *F > | 10.9 | 10-9 (F) *F > | 12.6 | 12-6 (D) *F > | 14.10 | 14-10 (F) *F > | 14.39 | 14-39 (L) *F > | 15.4 | 15-4 (B) *F > | 17.5 | 17-5 (M) *F > | 19.22 | 19-22 (N) *F > | 20.10 | 20-10 (O) *F > | 20.35 | 20-35 (F) *F > | 21.15 | 21-15 (J) *F > | 22.21 | 22-21 (C) *F > | 22.26 | 22-26 (I) *F > | 24.15 | 24-15 (P) *F > | 28.19 | not in Table 1 *F > | 29.26 | 29-26 (R) *F > | x-1 | Df(2R)X1 *F >------------------------------------------ *F >Fig9B | 8.1 | not in Table 1 *F > | 8.4 | 8-4 (B) *F > | 9.24 | 9-24 (B) *F > | 14.35 | 14-35 (B) *F > | 19.51 | 19-51 (B) *F > | 21.25 | not in Table 1 *F > | 21.30 | not in Table 1 *F > | 29.42 | 29-42 (B) *F > | 80.30 | not in Table 1 *F > | 83.21 | not in Table 1 *F >------------------------------------------ *F > *F >The difference seems to be that Currie and Sullivan used a '.' where *F >you used a '-'. I suspect that this means that the complementation *F >groups in Figure 9. of the Currie and Sullivan paper do correspond to *F >alleles listed in Table 1. of your paper, which means I could merge *F >several gene records in FlyBase. However, I wanted to make sure that my *F >hunch was correct before I do this. Do you have any information that *F >might help me confirm that the complementation groups given in Figure *F >9. of the Currie and Sullivan, 1994 paper are the same as in Table 1. *F >of your MGG paper, for example, did you give them these particular *F >stocks ? *F > *F these should all correspond to our abstracts (although minor updating *F occurred) and our MGG paper. mutants 1-11, 8-1 and 83-21 were removed *F when we became convinced they were a double mutants. .. *F 28-19 should be 28-20. *F > *F .. *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- *F > *F > *F > *F \-- *F Elliott S. Goldstein *F School of Life Science *F Cellular, Molecular and Bioscience Program *F Arizona State University *F Tempe, AZ 85287-4501 *F phone: 480-965-7176 *F fax: 480-965-6899 *F e.goldstein@asu.edu # *U FBrf0178745 *a Dow *b J.A.T. *t 2004.3.2 *T personal communication to FlyBase *u FlyBase error report for CG32089 on Tue Mar 2 06:42:55 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Tue, 2 Mar 2004 06:42:55 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: j.a.t.dow@bio.gla.ac.uk *F Subject: FlyBase error report for CG32089 on Tue Mar 2 06:42:55 2004 *F Error report from Julian Dow (j.a.t.dow@bio.gla.ac.uk) *F Gene or accession: CG32089 *F Gene annotation error *F Gene CG32089 corresponds to FBgn0028668 *F Comments: CG32089 is the gene I referred to as vha16-2 in : *F Dow, 1999 *F The multifunctional Drosophila melanogaster V-ATPase is encoded by a multigene *F family. J. Bioenerg. Biomembranes 31(1): 75--83  FBrf0108722 *F I suggest these entries are merged. # *U FBrf0178746 *a Dow *b J.A.T. *t 2004.3.2 *T personal communication to FlyBase *u FlyBase error report for CG32090 on Tue Mar 2 06:45:52 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Tue, 2 Mar 2004 06:45:52 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: j.a.t.dow@bio.gla.ac.uk *F Subject: FlyBase error report for CG32090 on Tue Mar 2 06:45:52 2004 *F Error report from Julian Dow (j.a.t.dow@bio.gla.ac.uk) *F Gene or accession: CG32090 *F Gene annotation error *F Gene CG32090 corresponds to FBgn0028667 *F Comments: CG32090 is the gene I referred to as vha16-3 in: *F Dow, 1999 *F The multifunctional Drosophila melanogaster V-ATPase is encoded by a multigene *F family. J. Bioenerg. Biomembranes 31(1): 75--83  FBrf0108722 *F I think these entries should be merged. # *U FBrf0178747 *a Dow *b J.A.T. *t 2004.3.2 *T personal communication to FlyBase *u FlyBase error report for CG30329 on Tue Mar 2 11:05:45 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Tue, 2 Mar 2004 11:05:45 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: j.a.t.dow@bio.gla.ac.uk *F Subject: FlyBase error report for CG30329 on Tue Mar 2 11:05:45 2004 *F Error report from Julian Dow (j.a.t.dow@bio.gla.ac.uk) *F Gene or accession: CG30329 *F Gene annotation error *F Gene CG30329 corresponds to FBgn0028669 *F Comments: CG30329 is the gene to which I referred as vha100-3 in: *F Dow, 1999 *F The multifunctional Drosophila melanogaster V-ATPase is encoded by a multigene *F family. J. Bioenerg. Biomembranes 31(1): 75--83 FBrf0108722 *F The apparent discrepancy in cytogenetic loci (55F cf. 56F) is due to the *F uncertainty in positioning the genomic clone at the time the paper was *F published. *F I suggest these records be merged. # *U FBrf0178748 *a Roberston *b H. *t 2004.3.30 *T personal communication to FlyBase *u *F Date: Tue, 30 Mar 2004 13:09:42 \-0600 *F From: Hugh Robertson *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: missing e-mail *F Thanks for remembering this, Gillian, here's the basic story. The three *F exon gene is below with introns in lower case. *F ATGGAACAGGCGTACAGTACAGCTTCCCGACTGCGTGGGATTTGCGTTTGTGGATTGGCCATTTCGGTGTATTCACTGTA *F TGTGAAAATGAAACTAAAGGAGGATGAGAACTATAGGCCAATGTGCGACGTTAACGATAATATAAGCTGCTCGCTGGTTT *F TTAAATCGGGgtaggtaagattataatatatacaatataaatttaatgaaataattttccatcagCTATGGTGATGGCTT *F TGGTCTGGGTAACATAACCCAAGTAAATGCTCCCAACGGAGCCATCGGCTGTGCATTTTATATCCTGTACTTCTTGAGCT *F gtacgtaaatttcttagtacaaactaaactaaatttaataagcatttgttttcagCTTTCTTTAATCATCGTTGGCTGTG *F CCTGGTCCAATTGATAGTATGCACTTTGACATTACTTCTCTGCGTTTATCTGGGTTTCCTGCTGATTCTCGTGTTTTACG *F ATTTCTGTTTGGTATGCGTGACCATCTATTTCATACACACATGGCTCTTTCAGGAAGTCCTAAGGCGATATAGACGCCTT *F TATATGTAA *F The encoded protein is *F MEQAYSTASRLRGICVCGLAISVYSLYVKMKLKEDENYRPMCDVNDNISCSLVFKSGYGDGFGLGNITQVNAPNGAIGCA *F FYILYFLSSFFNHRWLCLVQLIVCTLTLLLCVYLGFLLILVFYDFCLVCVTIYFIHTWLFQEVLRRYRRLYMZ *F This is the Drosophila homolog of the newly described human and other *F mammalian VKORC1 gene encoding vitamin K epoxide reductase. There are *F nice pseudoobscura and Anopheles orthologs, and the latter has ESTs. *F It is currently partly annotated by Hild et al in the TPA as HDC06808, *F however they splice from the middle of the third exon to a fourth *F distant downstream exon, which is not right and might even be a gene *F fusion. This downstream gene does have ESTs supporting it, so you might *F want to look into it too. *F There are no ESTs or any other data I can find for this gene in Drosophila. *F Thanks, *F Hugh # *U FBrf0178749 *a Desplan *b C. *t 2004.4.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Apr 2004 11:56:15 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Rh-lacZ insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Claude Desplan, New York University. *F P{Rh3-lacZ.PD}2, P{Rh5-lacZ.PD}2 and P{Rh6-lacZ.PD}2 are homozygous viable, *F second chromosome insertions. *F P{Rh5-lacZ.PD}3, P{Rh3-lacZ.PD}3 and P{Rh6-lacZ.PD}3 are third chromosome *F insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178750 *a Bonini *b N. *t 2004.4.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Apr 2004 12:05:29 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Hsap\MJD.tr-Q78}c37.3 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Nancy Bonini, University of Pennsylvania (2/04). *F P{UAS-Hsap\MJD.tr-Q78}c37.3 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178751 *a Wakimoto *b B. *t 2004.4.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Apr 2004 12:17:34 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{betaTub85D-CD2} construct and insertion *F The following information was provided to the Bloomington Stock Center by *F Barbara Wakimoto, University of Washington. *F P{betaTub85D-CD2} consists of the rat CD2 membrane protein driven by the *F betaTub85D promoter in a miniwhite-marked vector. It has been used to *F stain sperm tails with an anti-CD2 antibody. P{betaTub85D-CD2}2a is a *F homozygous viable and fertile, second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178752 *a Chang *b H. *t 2004.4.13 *T personal communication to FlyBase *u *F Date: Tue, 13 Apr 2004 15:09:11 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: Gene data (problem or question) *F comments: Dear Flybase, *F I think l(3)07086 is allelic to rtet. The evidence is that the l(3)07086 P *F line fails to complement to EP(3)3734, an allele of rtet. *F \-Henry *F realname: Henry Chang *F reply-to: henry@cellbiology.med.yale.edu *F source: FB-NG Help Mail: *F Date: Thu, 15 Apr 2004 10:45:47 \-0400 *F To: 'Gillian Millburn (Genetics)' *F From: Henry Chang *F Subject: Re: FB-NG Help Mail: Gene data (problem or question) *F Hi Gillian, *F Yes, I came across l(3)07086 first, from a screen for mutants *F exhibiting abnormal pattern of HRP-Boss (bride of sevenless) *F staining. In FLP-generated l(3)07086 mutant clones, HRP-Boss *F staining is absent. The same phenotype is also observed in EP(3)3734 *F mutant clones. The final data is that they fail to complement by *F lethality. *F So in short, they fail to complement by lethality and they both have *F identical clonal phenotype in HRP-Boss staining. *F \-H *F >Hi Henry, *F > *F >thanks for this information, I'll record your e-mail as a personal *F >communication to FlyBase and will merge the rtet and l(3)07086 gene *F >records. *F > *F >Can I just check what phenotype you were looking at when you were *F >looking at the failure of l(3)07086 and EP(3)3734 to complement \- were *F >you looking at lethality or another mutant phenotype ? (this extra info *F >would be useful to know and I'll add it to your personal *F >communication), *F > *F >Gillian *F > *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- *F > *F > *F >> *F >> comments: Dear Flybase, *F >> *F >> I think l(3)07086 is allelic to rtet. The evidence is that *F >>the l(3)07086 *F >P line fails to complement to EP(3)3734, an allele of rtet. *F >> *F >> \-Henry *F >> realname: Henry Chang *F >> reply-to: henry@cellbiology.med.yale.edu *F > > source: FB-NG Help Mail: *F > > *F \-- *F Henry Chang *F Department of Cell Biology *F Yale University School of Medicine *F 333 Cedar St. SHM C-435 *F New Haven, CT 06510 *F 203-785-6078 # *U FBrf0178753 *a Graveley *b B. *t 2004.4.19 *T personal communication to FlyBase *u FlyBase error report for CG7535 on Mon Apr 19 10:33:24 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Mon, 19 Apr 2004 10:33:24 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: graveley@neuron.uchc.edu *F Subject: FlyBase error report for CG7535 on Mon Apr 19 10:33:24 2004 *F Error report from Brenton Graveley (graveley@neuron.uchc.edu) *F Gene or accession: CG7535 *F Missed gene *F Comments: There is an alternative exon in this gene that is not annotated. *F The nucleotide sequence of this exon *F is...GCCACCAATGTGTCGGTGAACATGTTCCTGCGCTCCATTTCGAAAATCGACGACTACAAAATG. This *F exon is one of three mutually exclusive exons. The sequence for the other two *F exons, which are annotated, are *F ATGGTCCCGCCATAGTCAGAATCAATCTATTCGTTCGCAGTATTATGACGATTAGTGATATTAAAATG *F and ATGGTCCAGCTGTGGTGCGCGTCAACATATTCGTTAGGAGTATATCGAAAATCGATGATGTAACCATG. *F This new exon is supported by several references, and was most recently *F featured in Trends in Genetics (2004) Copley, Evolutionary convergence of *F alternative splicing in ion channels. # *U FBrf0178754 *a Graveley *b B. *t 2004.4.19 *T personal communication to FlyBase *u FlyBase error report for CG7535 on Mon Apr 19 11:05:25 2004. *F [FlyBase curator comment: this error report was originally submitted *F under the CG7535 gene. However, the sequence in it pertains to the slo *F gene (CG10693) and the text of the error report has been edited to *F reflect this.] *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Mon, 19 Apr 2004 11:05:25 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: graveley@neuron.uchc.edu *F Subject: FlyBase error report for CG10693 on Mon Apr 19 11:05:25 2004 *F Error report from Brenton Graveley (graveley@neuron.uchc.edu) *F Gene or accession: CG10693 *F Missed gene *F Comments: There are two additional alternative exons in this gene that are not *F annotated. These other exons are mutually exclusive with one exon that is *F currently annotated. The current exon is: *F GCAATGTTTGCCAGCAGTATACCGGAGATAATTGAACTCGTCGGTAGTGGCAATAAGTATGGCGGTGAACTGAAAAGAG *F AACACGGAAAGAG *F The two additional, mutually exclusive exons are: *F 1.GCCGTTTTCGCTAGTTGGATACCAGAAATCACTGAACTGGCTGCCCAAAGAAGCAAATATGGTGGAACATATAGCAA *F GGATCCTAGAAAAAG *F 2.GCGATTTTTGCAAGTTGTATACCTGAGATTATAGACTTGATTGGAACAAGAGCCAAATATGGGGGCACATTAAAAAA *F TGAAAAAGGGCGAAG *F This is most recently described in Copley (2004) Evolutionary convergence of *F alternative splicing in ion channels. Trends in Genetics. # *U FBrf0178755 *a Tao *b Y. *c J. *d True *t 2004.4.20 *T personal communication to FlyBase *u A note on the D. mauritiana Pw+ insert lines and their derivatives. *F A note on the D. mauritiana Pw+ insert lines and their derivatives *F Yun Tao (ytao@oeb.harvard.edu) *F April 20, 2004 *F The Pw+ insert lines of D. mauritiana constructed by True, Mercer and Laurie *F (1996, Genetics 142: 507-523) has been used in studying hybrid incompatibilities *F between D. mauritiana and D. simulans through an introgression approach (True, *F Weir *F and Laurie 1996, Genetics 142: 819-837; Tao, Hartl and Laurie 2001 PNAS 98: *F 13183-13188;Tao, Chen, Hartl and Laurie 2003, Genetics 164: 1383-1397; Tao, *F Zeng, *F Li, Hartl and Laurie 2003, Genetics 164: 1399-1418; Tao and Hartl 2003 *F Evolution 57: *F 2580-2598). It can be said that more follow-up studies will be published in *F the next *F few years (Y. Tao, J. P. Masly, L. Araripe, A. Orr, A. G. Clark, D. L. Hartl, *F unpublished work). In the next decade, the D. mauritiana Pw+ lines and their *F introgressive derivatives are likely to become valuable resources in the field *F of *F genetics of speciation. The D. mauritiana Pw+ stocks are now kept by J. P. *F Masly *F (graduate student in Allen Orr's lab of the University of Rochester) and Y. Tao *F (research associate with Dan Hartl of Harvard University). JPM and YT back up *F each *F other for these stocks, most of which have survived through the years. A list *F of the *F stocks still alive can be found in Tao (personal communication to FlyBase, *F 2002.12.8). *F Recently, Dr. Gillian Millburn, FlyBase curator, has risen a question about the *F nomenclature used for the D. mauritiana Pw+ stocks and their derivatives: *F Dear Dr. Tao, *F I am curating your paper for FlyBase: *F Tao et al., 2003, Genetics 164(4): 1383--1397 *F and I have a question about a few of the D.mauritiana insertion lines *F that were used to mark the introgressed segments \- whch are shown in *F Figure 4. *F 1. 21 71F72A *F in your personal communication to FlyBase: *F FBrf0155355 == Tao, 2002.12.8, personal communication to FlyBase *F there it lists information for the '71F72A' cytological position: *F line name       insert cyt pos *F 4Z2, 4Z3        71F72A *F when I curated this before, I took this to mean that there are 2 *F separate lines that have an insert at 71F-72A and made 2 separate *F insertion records \- one called 'P{lacW}mau-4Z2' for the 4Z2 strain and *F one called 'P{lacW}mau-4Z3' for the 4Z3 strain. Was I correct in this *F assumption \- are there 2 different inserts at 71F-72A in the 2 *F different lines ? *F If so, which line does '21 71F72A' from your Genetics paper *F correspond to ? *F I have contacted John True for this question: *F John, *F In the past several years, I have the honor of keeping your D. mau P lines as *F great *F resources for the Drosophila research community. Various questions have been *F risen *F on these lines, I have been able to answer most of them. However, occasionally *F there *F are nasty ones. Here is one example (see the text of Gillian's question): *F         In Gillian's question 1, line 71F72A (as in your Table A1 of Genetics *F 1996 paper) *F actually has two lines, 4Z2 and 4Z3, as physically labeled and kept for *F stocks. I *F re-labeled 71F72A as line \#21 in my Genetics 2003 paper. I need to go back my *F original lab notes to figure out which line 4Z2 or 4Z3 I actually used. Are *F 4Z2 and *F 4Z3 actually two independent lines? or duplicates of the same line? *F         The same questions apply to (F2, F3c 91A), (3A1 3A2 92E), (3Q3, 3Q3a *F 56DE) \- If *F they are duplicates, then it is easy to answer the questions. If they are *F separate *F inserts, then it may be very difficult to figure out which line you actually *F used in *F the Genetics 1996 paper \- even if Cathy still keeps your original notes. *F         Hope I have made the question clear. If not, let's talk through phone *F next week. I *F will be out of town this weekend and next Monday, so I may call you next *F Tuesday if *F I am still not sure. *F Best, *F Yun *F John's answer is clear (email to YT on April 16, 2004): *F Hi Yun, *F         lines with the same prefix (e.g. 4Z and F) are from the same G0 and *F are likely to be *F the same insert (this is pretty much assumed if they map to the same cytological *F location, but it was not checked molecularly).  Therefore, all of the groups you *F mentioned are assumed (and extremely likely to be \- although this is not *F confirmed *F at the molecular level) the same insert. *F John *F In our introgression studies (Tao, Hartl and Laurie 2001 PNAS 98: *F 13183-13188;Tao, *F Chen, Hartl and Laurie 2003, Genetics 164: 1383-1397; Tao, Zeng, Li, Hartl and *F Laurie 2003, Genetics 164: 1399-1418; Tao and Hartl 2003 Evolution 57: *F 2580-2598), *F three of the four lines in question have been used. I went back to my old *F notes and *F found the following connections (see Tao and Hartl 2003 Evolution 57: *F 2580-2598 for *F a full list of all the introgression lines mentioned): *F 1. All lines of Pw+ \#21 were derived from 4Z3 71F72A; *F 2. Lines of 44.3, 44.5 and 44B were derived from 3A1 92E, while 44A was from *F 3A2 92E; *F 3. Lines of 41A, 41.1, 41.2, 41.3 and 41.4 were derived from F2 91A, while *F 41.5 and 41.6 were derived from F2c 91A. *F Whether these pairs of Pw+ are the same is inconsequential for all the work *F published so far. The insertion sites of these lines can be sequenced through *F inverse PCR \- which can be easily done if necessary. # *U FBrf0178756 *a Baum *b B. *t 2004.3.2 *T personal communication to FlyBase *u *F Date: Fri, 20 Feb 2004 15:28:13 \+0000 (GMT) *F From: 'Gillian Millburn (Genetics)' *F Subject: FlyBase query *F To: b.baum@ucl.ac.uk *F Hi Buzz, *F I'm curating your paper for FlyBase: *F Kunda et al., 2003, Curr. Biol. 13(21): 1867--1875 *F .. *F 1. Arc-p20 and HSPC300. *F could you tell me which CG each of these genes correspond to, so that I *F can rename the CGs in FlyBase. *F .. *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- *F Date: Tue, 02 Mar 2004 21:42:05 \+0000 *F To: 'Gillian Millburn (Genetics)' *F From: Buzz Baum *F Subject: Re: FlyBase query *F Dear Gillian, *F .. *F CG30173 = HSPC300 homologue *F CG5972 = Arp-p20 *F .. *F Cheers *F Buzz *F At 03:28 PM 2/20/04 \+0000, you wrote: *F >Hi Buzz, *F > *F >I'm curating your paper for FlyBase: *F > *F >Kunda et al., 2003, Curr. Biol. 13(21): 1867--1875 *F > *F .. *F > *F >1. Arc-p20 and HSPC300. *F > *F >could you tell me which CG each of these genes correspond to, so that I *F >can rename the CGs in FlyBase. *F > *F > *F .. *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- # *U FBrf0178757 *a Brody *b T. *t 2004.4.27 *T personal communication to FlyBase *u *F Date: Tue, 27 Apr 2004 15:21:18 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: Tom Brody *F Subject: incorrectly defined gene *F Dear FlyBasers: *F I would like to call your attention to two genes that are incorrectly *F defined in FlyBase. They are CG15000 and CG15001. *F These CGs really constitute one gene, as defined correctly by *F Clements M, Duncan D, Milbrandt J (2003). Drosophila NAB (dNAB) is an *F orphan transcriptional co-repressor required for correct CNS and eye *F development. Dev Dyn.226(1): 67-81 *F I have verified their definition of that gene by partial sequencing *F of the EST LP22227 of the BDGP. Please note the 5' sequence of this *F EST, available from the BDGP database, covers CG15001, but the end of *F that EST, by blast search, overlaps CG15000. I have now done a 3' *F sequence of that EST, and its sequence runs all the way through the *F 3' UTR of CG15000, the downstream gene. *F Below is my sequence using the proper primer for 3' sequence for that *F EST: This shows the 3' reach of the combined CG15001 (upstream) and *F CG15000 (downstream) sequence. *F TTTTTTTTTTTTTTTTTAAAATTATTATAACTTTTTATTGNATAGCATAAATAATGATTTTATTACAGTGTTTTAGTAT *F AGATCACAGNAGNACTACAAAATAATCGTTGTCTTAAAGCTCCTTAAATAAAGTTATGTTTTTAGACGATACTAATCTA *F GTTGAATTGCACTGGAATCAAAACGATTTGGGTGTGGGTAACCCAATCTGGCACTACGAATGGCATCCATCCAATTTTC *F TCCAGAACTAAGTCTCTGGNGAAGCAGCACTCCTCTTGAGCGCGAGCACCTCGTTCAAAGCGGGACTGAGTGCCAGGGC *F GGGATTG *F Sincerely, *F Tom Brody *F \-- # *U FBrf0178758 *a Schaeffer *b S.W. *t 2004.1.1 *T personal communication to FlyBase *u *F Date: Thu, 01 Apr 2004 15:37:56 \-0500 *F To: 'Michael Ashburner (Genetics)' *F From: 'Stephen W. Schaeffer' *F Subject: Re: Jonah99 *F Dear Michael, *F .... *F By the way, the real Jonah sequences identified by Carlson and Hogness *F (1985, Developmental Biology, 108:341-354) are serine proteases. I wanted *F to sort out Janet Collett's Jonah 99C sequence so I went back to original *F papers. I was able to infer what these sequences were by creating a *F database of approximately 200 kilobases around the putative genetic *F locations of the seven Jonah locations. I search each region against all *F of the other six regions and was able to find members of the gene *F family. Based on their restriction maps and the locations of the serine *F proteases with respect to the maps, I made the following identifications: *F Jonah 25B (3 genes: Ser4, CG8869, CG8871) *F Jonah 44E (1 gene: CG8579) *F Jonah 65A (7 genes: CG10475, CG6580, CG6483, CG6467, CG6462, yip7, CG10477) *F Jonah 66C (2 genes: CG7118, CG7170) *F Jonah 99C Beta (3 genes: Ser99Dc, Ser99Da, Ser99Db) *F Jonah 99C Alpha (I wasn't sure of the gene ID here) *F Jonah 99F (2 genes: CG18030, CG2229) *F One problem is that Carlson and Hogness identified a subset of the Jonah's in *F their midgut cDNA screen and I bet that there are other members of the *F family that already have different names. *F .... *F Steve # *U FBrf0178759 *a Levis *b R. *t 2001.9.28 *T personal communication to FlyBase *u *F Personal communication from Robert Levis, 28 September 2001 *F 1) BG02205 *F The available data suggest that this is a single insertion, since the *F 3' and 5' flanks have an 8 bp target site duplication and map to the *F same site in the prototype 1360/hoppel element. Guochun's software *F called it multiple, meaning that it hit multiple sites in the genome *F (i.e. it is repetitive, not a multiple insert). While most of both *F flanks is repetitive, there is a region of the 5' flank from 270 to *F ~410 that hits a single scaffold segment, AE003569. It was on this *F basis that I mapped it to 19F. This might be incorrect if this *F apparently unique segment is actually repeated elsewhere in the *F portion of the genome that is not represented in the whole genome *F assembly. *F 2) BG02303 *F The 5' and 3' flanking sequence reads for this insertion map 3.4kb *F apart. From the available data it cannot be determined whether this *F is two insertions or a single insertion associated with a deletion. *F 3) BG02678 *F The 5' flanking sequence aligns to the blastopia repetitive element, *F while the 3' flanking sequence maps to a single site in 2R-57C. The *F absence of a target site duplication suggests two insertions (or an *F insertion associated with a deletion). # *U FBrf0178760 *a Rana *b D. *t 2004.7.2 *T personal communication to FlyBase *u *F Date: Thu, 08 Jul 2004 12:30:17 \+0100 *F From: Debashis Rana *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: help with another BLAST ? *F Hi Gillian, *F .. This *F data was generated with dmel_all_transcripts_3.2.0 dataset with an e *F value of 100. *F Cheers, *F Debashis *F FlyBase curator comment: BLAST output file archived as Rana.2004.7.2-1.txt *F File deposited: Rana.2004.7.2-1.txt ; File date: 2004.7.16 ; File size: *F 2242679 ; File format: txt # *U FBrf0178761 *a Ashburner *b M. *t 2004.6.30 *T personal communication to FlyBase *u *F Date: Wed, 30 Jun 2004 16:44:55 \+0100 (BST) *F From: 'Michael Ashburner (Genetics)' *F Subject: Re: Liang anon-EST's *F To: michael@morgan.harvard.edu, crosby@morgan.harvard.edu *F Cc: rd120@gen.cam.ac.uk, gm119@gen.cam.ac.uk *F ... *F See attached file for Lang EST cleanup. *F File as a pc and do the merges needed. For those that are *F not in genome or are plasmid vector I suggest add a note and *F these must be status_uncertain *F Q's to me *F Michael *F Executive summary; *F anon-EST:Liang-1.11 == CG9022? *F anon-EST:Liang-1.13 = CG11798 *F anon-EST:Liang-1.16 = CG31738 *F anon-EST:Liang-1.18 == CG11107 *F anon-EST:Liang-1.21 == CG5931 *F anon-EST:Liang-1.24 = CG32743 *F anon-EST:Liang-1.25 = CG11525 *F anon-EST:Liang-1.3 = ? something new ? *F anon-EST:Liang-1.34 = ? something new ? *F anon-EST:Liang-1.38 == something new ? *F anon-EST:Liang-1.39 = no sequence data available *F anon-EST:Liang-1.4 = CG7977 *F anon-EST:Liang-1.40 == ? not in genome *F anon-EST:Liang-1.43 = CG5720 *F anon-EST:Liang-1.52 = CG3998 *F anon-EST:Liang-1.53 = looks like bacterial plasmid seq to me *F anon-EST:Liang-1.56 = CG7808 *F anon-EST:Liang-1.62 no sequence data available *F anon-EST:Liang-1.66 = CG4978 *F anon-EST:Liang-1.72 = CG12011 *F anon-EST:Liang-1.74 = CG14938 *F anon-EST:Liang-1.75 = CG5481 *F anon-EST:Liang-1.76 = CG31363 *F anon-EST:Liang-1.79 = CG5395 *F anon-EST:Liang-1.80 == CG14996 *F anon-EST:Liang-1.83 = CG7055 *F anon-EST:Liang-2.1 no sequence data available *F anon-EST:Liang-2.12 = CG17841 *F anon-EST:Liang-2.13 = ? on hth intron ? *F anon-EST:Liang-2.14 = sd? *F anon-EST:Liang-2.15 == CG15100 *F anon-EST:Liang-2.16 = CG4602? match bad, but note n's in query seq *F >anon-EST:Liang-2.19 = CG32177 *F anon-EST:Liang-2.2 = CG15154 *F anon-EST:Liang-2.23 = roo element *F anon-EST:Liang-2.24 == CG11525 *F anon-EST:Liang-2.28 = CG2210 *F anon-EST:Liang-2.31 = not in genome *F anon-EST:Liang-2.39 = in how intron ? *F anon-EST:Liang-2.40 = no sequence data available *F anon-EST:Liang-2.41 = CG32662 *F anon-EST:Liang-2.42 = CG5014 *F anon-EST:Liang-2.47 = CG1499 *F anon-EST:Liang-2.48 = CG14938 *F anon-EST:Liang-2.49 = CG3821 *F anon-EST:Liang-2.51 == CG7533 (charybde) (1-635 only; chimeric? Last *F anon-EST:Liang-2.54 == CG9894 *F anon-EST:Liang-20 == CG10035 *F anon-EST:Liang-38 == CG17579 (sca) *F anon-EST:Liang-97 == CG3359 (1-468 only; chimeric cDNA? Last 300bp not *F identifiable \-- het?) *F Michael *F \# *F 1. Blast at FB vs Predicted genes NT *F 2. If no: Blast at FB vs euchromatic and heterochromatic scaffolds NT *F 2. Blast Drosophila TE's at FB *F 4. If no Blast at FB all Drosophila NT *F 5. Blastn at NCBI *F \# *F anon-EST:Liang-1.11 == CG9022? *F >anon-EST:Liang-1.11 *F aaaantggnc ataatggtng tgnantangc atagagcggt gtgttnnata agntggnngg *F taatcngaac ntngtngagt ntattatnaa ntgggtattt ggngagantg gtntnttgnn *F nnnggcatnn gtgtaagatt ataaggaggg gganntgntg ttacgggata aggnn *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003446|gb|AE003446|arm:X 8788012..9078081 *F estimated-cyto:8C4-8E1 gadfly-seqname:AE003446 *F seq_release:3 *F Length = 290070 *F Score = 81.4 bits (42), Expect = 6e-15 Genome Map *F Identities = 107/154 (69%) *F Strand = Plus / Minus *F Query: 20 gtgnantangcatagagcggtgtgttnnataagntggnnggtaatcngaacntngtngag 79 *F ||| | || ||| ||||||||||||| | ||| ||| ||||||| || | | ||| *F Sbjct: 108565 gtgcagtacgcacagagcggtgtgttccacaagctggccggtaatcgcgacgtggccgag *F 108506 *F Query: 80 tntattatnaantgggtatttggngagantggtntnttgnnnnnggcatnngtgtaagat 139 *F | |||| || ||||||||||| |||| ||| ||| ||||| ||| || || *F Sbjct: 108505 tccattagcaagtgggtatttggagagactggccgcttgcgcgtggcatccgtgcaacat *F 108446 *F Query: 140 tataaggaggggganntgntgttacgggataagg 173 *F | |||||||| || || || || |||| ||| *F Sbjct: 108445 cacaaggagggcgagctgctgccaccggatcagg 108412 *F BLAST against: Database: dmel_all_transcript_r320 *F >CG9022-RA type=mRNA; *F loc=X:complement(8895906..8897199,8897274..8897495); Genome Map *F ID=Ost48-RA; name=Ost48-RA; *F db_xref='CG9022,FlyBase:FBgn0014868'; len=1516 *F Length = 1516 *F Score = 81.4 bits (42), Expect = 2e-15 *F Identities = 107/154 (69%) *F Strand = Plus / Plus *F Query: 20 gtgnantangcatagagcggtgtgttnnataagntggnnggtaatcngaacntngtngag 79 *F ||| | || ||| ||||||||||||| | ||| ||| ||||||| || | | ||| *F Sbjct: 846 gtgcagtacgcacagagcggtgtgttccacaagctggccggtaatcgcgacgtggccgag 905 *F Query: 80 tntattatnaantgggtatttggngagantggtntnttgnnnnnggcatnngtgtaagat 139 *F | |||| || ||||||||||| |||| ||| ||| ||||| ||| || || *F Sbjct: 906 tccattagcaagtgggtatttggagagactggccgcttgcgcgtggcatccgtgcaacat 965 *F Query: 140 tataaggaggggganntgntgttacgggataagg 173 *F | |||||||| || || || || |||| ||| *F Sbjct: 966 cacaaggagggcgagctgctgccaccggatcagg 999 *F Database: na_all.dros *F >BACR19P22-T7 (STS) made from BACR19P22 *F Length = 809 *F Score = 81.4 bits (42), Expect = 3e-14 *F Identities = 107/154 (69%) *F Strand = Plus / Plus *F Query: 20 gtgnantangcatagagcggtgtgttnnataagntggnnggtaatcngaacntngtngag 79 *F ||| | || ||| ||||||||||||| | ||| ||| ||||||| || | | ||| *F Sbjct: 476 gtgcagtacgcacagagcggtgtgttccacaagctggccggtaatcgcgacgtggccgag 535 *F Query: 80 tntattatnaantgggtatttggngagantggtntnttgnnnnnggcatnngtgtaagat 139 *F | |||| || ||||||||||| |||| ||| ||| ||||| ||| || || *F Sbjct: 536 ttcattagcaagtgggtatttggagagactggccgcttgcgcgtggcatccgtgcaacat 595 *F Query: 140 tataaggaggggganntgntgttacgggataagg 173 *F | |||||||| || || || || |||| ||| *F Sbjct: 596 cacaaggagggcgagctgctgccaccggatcagg 629 *F \# *F anon-EST:Liang-1.13 = CG11798 *F >anon-EST:Liang-1.13 *F aaaagcttgc ttgttctttt tgcagaagct cagaataaac gctcaacttt gggacctgca *F cccccccccc cccccctcga tcgctcggca gcgcccagca ttgaatacga gtccagtgcg *F gccggtgcct ctggcaacaa cttggccacc acccaggcca atgtaatcca caacaacagc *F agcagcaaca gcaagcggag tcgggcaact ccgtggtggt gacggccaca gtggggcgac *F tgtggtgccg gcacccagtg tatcggccgt tggtggcttc aagtccgagg atcatctgag *F cactgccttc gggctggcag ccctgatgca aacggtttcg caccggccag gcgggtctgc *F tgaaaccggc gagcaacaaa gcgctgggct caggacggat ctggtctggt ggcgccgcac *F cggcggaacc acaactcgtg cagtggactc gggcggaaag ctccaagcta cgctcatgtc *F aaccaacacg cacactaata acccgcatag acatccaatc aaaaaacacc taggaacatg *F cggctgaatt gattaccacc ccccacatcg ggcaatgcgg gccaaaatct tggcccaaat *F ccatatccac cttagcgccc atgtaatatg cgttgggggc tcacactcct ccggtggaaa *F gaagggtccg aaaaggccct accgttttcc gcc *F Database: dmel_all_transcript_r320 *F >CG11798-RA type=mRNA; *F loc=2R:join(10192641..10192910,10201827..10202161, *F 10202234..10202706,10202804..10203820, *F 10205562..10208508); ID=CG11798-RA; name=CG11798-RA; *F db_xref='CG11798,FlyBase:FBgn0033992'; len=5042 *F Length = 5042 *F Genome Map *F Score = 642 bits (334), Expect = 0.0 *F Identities = 407/421 (96%), Gaps = 9/421 (2%) *F Strand = Plus / Plus *F Query: 77 tcgatcgctcggcagcgcccagcattgaatacgagtccagtgcggccggtgcctctggca 136 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 613 tcgatcgctcggcagcgcccagcattgaatacgagtccagtgcggccggtgcctctggca 672 *F Query: 137 acaacttggccaccacccaggccaatgtaatcca-caacaacagcagcagcaacagcaag 195 *F ||||| |||||||||||||||||||||||||||| |||||||| |||||||||||||||| *F Sbjct: 673 acaacgtggccaccacccaggccaatgtaatccagcaacaacaacagcagcaacagcaag 732 *F Query: 196 cggagtcgggcaactccgtggtggtgacggcca-cagtggggcgactgtggtgccggcac 254 *F ||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||| *F Sbjct: 733 cggagtcgggcaactccgtggtggtgacggccagcagtggggcgactgtggtgccggcac 792 *F Query: 255 ccagtgtatcggccgttggtggcttcaagtccgaggatcatctgagcactgccttcgggc 314 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 793 ccagtgtagcggccgttggtggcttcaagtccgaggatcatctgagcactgccttcgggc 852 *F Query: 315 tggcagccctgatgca-aacggtttcgca-ccggccaggcgggtctgctgaaa-ccggcg 371 *F |||||||||||||||| |||||||||||| ||||||||||||||||||||||| |||||| *F Sbjct: 853 tggcagccctgatgcagaacggtttcgcagccggccaggcgggtctgctgaaagccggcg 912 *F Query: 372 agcaacaa-agcgctgggctcaggacggatctggtctggtggc-gccgcaccggcggaac 429 *F |||| ||| |||||||||||||||||||||||||||||||||| |||||| ||||||||| *F Sbjct: 913 agcagcaacagcgctgggctcaggacggatctggtctggtggcggccgcagcggcggaac 972 *F Query: 430 cacaactcgtgcagtgga-ctcgggcggaaagctcca-agctacgctcatgtcaaccaac 487 *F |||||||||||||||||| |||||||||||||||||| |||||||||||||||||||||| *F Sbjct: 973 cacaactcgtgcagtggacctcgggcggaaagctccagagctacgctcatgtcaaccaac 1032 *F Query: 488 a 488 *F | *F Sbjct: 1033 a 1033 *F \# *F anon-EST:Liang-1.16 = CG31738 *F >anon-EST:Liang-1.16 *F agcagttcca gttccctaaa actgggctgg gagcggcggg ctcaagatgg tgacttcctg *F ctgcaactac aagatgcaga aacaggtcat ggtttcctca atacctacaa gggtacagag *F ctgcaaacgg agtgcctgca attaaggcgg gctagcagct accaattccg tctgagatcc *F gaaaacgaag ccggtttttc accctggtca ccggaggtta gctaccgcac tttagcggaa *F cgcccgggaa ggccagggaa accgcatgcc aagggaaaga ttcatggcac tcagtttaag *F gcccgctggg atgcacccag cgattccggt ggtgctgaga tcctgtgcta ccacttggag *F ttaagtgccg ctgggccaac tttcgaaagg atctacagtg gcgccgaaac agaagccatg *F tgcgaaagac ttcagccggg taccacatac gctctgcgag cctgttgcga angtccaagc *F tggtcagagt ccgtactccg atataggaca agtcaccacn gaaggcggtg ccaccaatct *F gctcccgcct ccaaccgcaa ttgcagtgat cctccctcgc cgtacgccgc ccctactgaa *F gctccggggc tcccggaata taaacngtng nngctccnga ttttaaaagt ttgnagcccc *F aaaatccgtt cncctaanag cnaagttgcn agccngcccg cantttgggt ttaccgccgg *F naaagcanaa cctanctttg ttggcccaag gatnttgaaa accc *F Database: dmel_all_transcript_r320 *F >CG31738-RA type=mRNA; *F loc=2L:join(16635199..16635513,16635583..16636036, *F 16636108..16640110); ID=CG31738-RA; name=CG31738-RA; *F db_xref='CG31738,FlyBase:FBgn0051738'; len=4772 *F Length = 4772 *F Genome Map *F Score = 1040 bits (541), Expect = 0.0 *F Identities = 596/607 (98%), Gaps = 7/607 (1%) *F Strand = Plus / Plus *F Query: 1 agcagttccagttccctaaaactgggctgggagcggcgggctcaagatggtgacttcctg 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1747 agcagttccagttccctaaaactgggctgggagcggcgggctcaagatggtgacttcctg 1806 *F Query: 61 ctgcaactacaagatgcagaaacaggtcatggtttcctcaatacctacaagggtacagag 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1807 ctgcaactacaagatgcagaaacaggtcatggtttcctcaatacctacaagggtacagag 1866 *F Query: 121 ctgcaaacggagtgcctgcaattaaggcgggctagcagctaccaattccgtctgagatcc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1867 ctgcaaacggagtgcctgcaattaaggcgggctagcagctaccaattccgtctgagatcc 1926 *F Query: 181 gaaaacgaagccggtttttcaccctggtcaccggaggttagctaccgcactttagcggaa 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1927 gaaaacgaagccggtttttcaccctggtcaccggaggttagctaccgcactttagcggaa 1986 *F Query: 241 cgcccgggaaggccagggaaaccgcatgccaagggaaagattcatggcactcagtttaag 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1987 cgcccgggaaggccagggaaaccgcatgccaagggaaagattcatggcactcagtttaag 2046 *F Query: 301 gcccgctgggatgcacccagcgattccggtggtgctgagatcctgtgctaccacttggag 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2047 gcccgctgggatgcacccagcgattccggtggtgctgagatcctgtgctaccacttggag 2106 *F Query: 361 ttaagtgccgctgggccaactttcgaaaggatctacagtggcgccgaaacagaagccatg 420 *F |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2107 ttaagtgccgctgggccagctttcgaaaggatctacagtggcgccgaaacagaagccatg 2166 *F Query: 421 tgcgaaagacttcagccgggtaccacatacgctctgcgagcctgttgcgaangtccaagc 480 *F ||||||||||||||||||||||||||||||||||||||||||||||||||| |||| ||| *F Sbjct: 2167 tgcgaaagacttcagccgggtaccacatacgctctgcgagcctgttgcgaaggtcc-agc 2225 *F Query: 481 tggtcagagtccgtactccgatataggacaagtcaccacngaaggcggtgccaccaatct 540 *F |||||||||||||||||||||||||||||| |||||||| | ||||||||||||| |||| *F Sbjct: 2226 tggtcagagtccgtactccgatataggacacgtcaccacgg-aggcggtgccacc-atct 2283 *F Query: 541 gctcccgcctccaaccgcaattgcagtgatcctccctcgccgtacgccgcccctactgaa 600 *F ||| |||||||| ||||| |||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 2284 gct-ccgcctcc-accgc-attgcagtgatcctccctcgccgtacgccg-ccctactgaa 2339 *F Query: 601 gctccgg 607 *F ||||||| *F Sbjct: 2340 gctccgg 2346 *F \# *F anon-EST:Liang-1.18 == CG11107 *F \# *F anon-EST:Liang-1.21 == CG5931 *F \# *F anon-EST:Liang-1.24 = CG32743 *F >anon-EST:Liang-1.24 *F aaagtacaga caaactgacg gacacggatt tgctgtggac cggaagccgt tggatcgtgg *F catatgccac gtgctctctc ggatcgcaat ccgcattttg aattccgagt ggacataacg *F agttgctggc cgagtagcag cagatgcagc ggctgacaca gcctcctata gctcccgatg *F taagagatct gggctcgatg acgttatcgg atacgatata ttccaggttt tagatacaaa *F attttcgttt accggccctc cgttatattt cctttttttt ttttaaaatg ctgttgtgca *F acttttccta tacgattcga ttataaacta tatattaatt tacaccatac cggaagtgtt *F ttttttttat tacgtgttaa gccactctca gtttcagccg ccttccccca ctcccctcta *F aaaccaaatc tgtagccgca ttagaactat atgtacaacc caatatatat atatatatat *F atatatatat gtatatgtat atccgcgatg cccgttcata gccgatgtcg gctatgcccg *F cccgttctcg ttttagttat ttcaataaac acaatgctga atataaatat aaatgttcgt *F ttccttcgag tgtaagtgag ctaanaactt tttgtgggaa taaaatcact cattttaatt *F ttctaaaagt tcaanccccg aattgcgcat cccgtttttt actt *F CG32743-RA type=mRNA; *F loc=X:join(6565014..6565362,6565432..6565795, *F 6565866..6568125,6568194..6571694,6574241..6574560, *F 6574626..6575970,6576021..6576989,6577057..6577405, *F 6577477..6578626); ID=Smg1-RA; name=Smg1-RA; *F db_xref='CG32743,FlyBase:FBgn0052743'; len=10607 *F Length = 10607 *F Genome Map *F Score = 756 bits (393), Expect = 0.0 *F Identities = 434/463 (93%), Gaps = 1/463 (0%) *F Strand = Plus / Plus *F Query: 1 aaagtacagacaaactgacggacacggatttgctgtggaccggaagccgttggatcgtgg 60 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 9918 aaagtacagacagactgacggacacggatttgctgtggaccggaagccgttggatcgtgg 9977 *F Query: 61 catatgccacgtgctctctcggatcgcaatccgcattttgaattccgagtggacataacg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 9978 catatgccacgtgctctctcggatcgcaatccgcattttgaattccgagtggacataacg 10037 *F Query: 121 agttgctggccgagtagcagcagatgcagcggctgacacagcctcctatagctcccgatg 180 *F |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 10038 agttgctggccgagtagcagcagatgcagcggcttacacagcctcctatagctcccgatg 10097 *F Query: 181 taagagatctgggctcgatgacgttatcggatacgatatattccaggttttagatacaaa 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 10098 taagagatctgggctcgatgacgttatcggatacgatatattccaggttttagatacaaa 10157 *F Query: 241 attttcgtttaccggccctccgttatattt-ccnnnnnnnnnnnnaaaatgctgttgtgc 299 *F |||||||||||||||||||||||| ||||| || |||||||||||||| *F Sbjct: 10158 attttcgtttaccggccctccgttttatttccctttttttttttttaaatgctgttgtgc 10217 *F Query: 300 aacttttcctatacgattcgattataaactatatattaatttacaccataccggaagtgn 359 *F ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| *F Sbjct: 10218 aacttttcctatacgattcgattataaactatatattaatttacaccatactggaagtgt 10277 *F Query: 360 nnnnnnnnnattacgtgttaagccactctcagtttcagccgccttcccccactcccctct 419 *F |||||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 10278 tttttttttattacgtgttaagccactcttagtttcagccgccttcccccactcccctct 10337 *F Query: 420 aaaaccaaatctgtagccgcattagaactatatgtacaaccca 462 *F ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 10338 aaaaccaaatctgtagccgcattagaactatatgtacaaccca 10380 *F \# *F anon-EST:Liang-1.25 = CG11525 *F >anon-EST:Liang-1.25 *F gagggccggt atgggacacc gggtgctgct ggcttggaat atcagaagta cgaacaacaa *F caacaactgg aggatctggc ggagtccgag gcaggagccg tcggtggagc cagcaacaac *F aacggcgaat cgtcgtcgtc cttgaaaaag ctagaggatc agctgcacgc cctcacctcg *F gacgagttgt acgaaaccct caaggagtac gacgtcctgc aggacaagtt ccacacggtg *F ctgctgttgc ccaaggaatc aaggcgtgag gttactgccg gaggacgaga tggatccgcc *F tacgtgctgc gctgcctgaa gatgtggtac gagctgccct ccgacgtcct gttctcggcc *F atgagcctgg tggaccgctt cctggatcgc atggccgtca agccgaagca catggcctgc *F atgagcgtgg cctcgttcca cttggccatc aagcagctgg acttgaaacc cattcccgcc *F gaggatctgg ttacaatatc tcantgtggt tgtaccgctg gtgatctgga acgcaaggcc *F ggcgtngatt gccaacaaan ctgggcgttc aagatgggac atgcaccgat taacntctgt *F gagctacctg gcgcatctac taangcccct cttccgcaaa ctttggncga aaggannatt *F tggggggnga acttttttaa agnttctaac aaacaa *F Database: dmel_all_transcript_r320 *F >CG11525-RD type=mRNA; *F loc=3R:complement(27413538..27414436,27415477..27415691, *F 27415762..27416136,27416200..27416439,27416504..27417318, *F 27427399..27427752); ID=CycG-RD; name=CycG-RD; *F db_xref='CG11525,FlyBase:FBgn0039858'; len=2898 *F Length = 2898 *F Genome Map *F Score = 1092 bits (568), Expect = 0.0 *F Identities = 624/639 (97%), Gaps = 7/639 (1%) *F Strand = Plus / Plus *F Query: 1 gagggccggtatgggacaccgggtgctgctggcttggaatatcagaagtacgaacaacaa 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 906 gagggccggtatgggacaccgggtgctgctggcttggaatatcagaagtacgaacaacaa 965 *F Query: 61 caacaactggaggatctggcggagtccgaggcaggagccgtcggtggagccagcaacaac 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 966 caacaactggaggatctggcggagtccgaggcaggagccgtcggtggagccagcaacaac 1025 *F Query: 121 aacggcgaatcgtcgtcgtccttgaaaaagctagaggatcagctgcacgccctcacctcg 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1026 aacggcgaatcgtcgtcgtccttgaaaaagctagaggatcagctgcacgccctcacctcg 1085 *F Query: 181 gacgagttgtacgaaaccctcaaggagtacgacgtcctgcaggacaagttccacacggtg 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1086 gacgagttgtacgaaaccctcaaggagtacgacgtcctgcaggacaagttccacacggtg 1145 *F Query: 241 ctgctgttgcccaaggaatcaaggcgtgaggttactgccggaggacgagatggatccgcc 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1146 ctgctgttgcccaaggaatcaaggcgtgaggttactgccggaggacgagatggatccgcc 1205 *F Query: 301 tacgtgctgcgctgcctgaagatgtggtacgagctgccctccgacgtcctgttctcggcc 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1206 tacgtgctgcgctgcctgaagatgtggtacgagctgccctccgacgtcctgttctcggcc 1265 *F Query: 361 atgagcctggtggaccgcttcctggatcgcatggccgtcaagccgaagcacatggcctgc 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1266 atgagcctggtggaccgcttcctggatcgcatggccgtcaagccgaagcacatggcctgc 1325 *F Query: 421 atgagcgtggcctcgttccacttggccatcaagcagctggacttgaaacccattcccgcc 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1326 atgagcgtggcctcgttccacttggccatcaagcagctggacttgaaacccattcccgcc 1385 *F Query: 481 gaggatctggttacaatatctcantgtggttgtaccgctggtgatctggaacgcaaggcc 540 *F ||||||||||||||||||||||| ||||||||||||||||||||||||||||||| |||| *F Sbjct: 1386 gaggatctggttacaatatctcagtgtggttgtaccgctggtgatctggaacgcatggcc 1445 *F Query: 541 ggcgtngattgccaacaaanctgggcgttcaagatgggacatgcaccgattaacntctgt 600 *F ||||| |||||||||| || ||||||| || |||||||||||||||||| | || ||||| *F Sbjct: 1446 ggcgt-gattgccaac-aagctgggcg-tccagatgggacatgcaccga-tcacttctgt 1501 *F Query: 601 gagctacctggcgcatctactaangcccctcttccgcaa 639 *F ||||||||| |||||||||||| ||||||||||||| *F Sbjct: 1502 gagctacct-gcgcatctactacg--ccctcttccgcaa 1537 *F \# *F anon-EST:Liang-1.3 = ? something new ? *F >anon-EST:Liang-1.3 *F attagcctgc gagcaggcaa cagatggcac atcacatgca atccacatac ccccatacca *F aaccatacca ttccatacta taccatcctc caatcctccg atcctccgat cttgcaatct *F tggtgaactt gagcgcattg cgatgtgctc atatatttct tgctgctcgt tgaggatgat *F gggaaaactc gtttttcgtt gcattcttcg gccggcgtca tcgatgatgc tgataatgat *F aatgataatg acaatgataa taatgatgat aatattaaag aggttccatt ggcataagtc *F cggctcgatg atgatcgcga tttgccttgg cacaacgcaa tgccaaagtt gttgtgccgg *F ttgttgcctt ccagctccta tatatatata catacagata tactatgtac atatgttttt *F tcttttattt tctttgtccg gggcactctt gtctcccggg ttccgttcta cagacgacct *F taaggagggg gtcttgggag gccagtcggg agactggagc aaaccacatt tcgtattccg *F tanacctggc gaaatattcg atnacatgcg attttcaaca accagaggac attgtttcta *F ttgaaatata ataaaataag taaatatggt atgtaacang aaaaagttta agctttgatg *F attgttttgt tacaanagag cacgtcttaa gaagttttta ttcaaggcaa aangacaaat *F gagggccttt tttttttttt c *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003490|gb|AE003490|arm:X 12354616..12657280 *F estimated-cyto:11B5-11D3 gadfly-seqname:AE003490 *F seq_release:3 *F Length = 302665 *F Score = 431 bits (224), Expect = e-119 Genome Map *F Identities = 224/224 (100%) *F Strand = Plus / Minus *F Query: 1 attagcctgcgagcaggcaacagatggcacatcacatgcaatccacatacccccatacca 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268279 attagcctgcgagcaggcaacagatggcacatcacatgcaatccacatacccccatacca *F 268220 *F Query: 61 aaccataccattccatactataccatcctccaatcctccgatcctccgatcttgcaatct 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268219 aaccataccattccatactataccatcctccaatcctccgatcctccgatcttgcaatct *F 268160 *F Query: 121 tggtgaacttgagcgcattgcgatgtgctcatatatttcttgctgctcgttgaggatgat 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268159 tggtgaacttgagcgcattgcgatgtgctcatatatttcttgctgctcgttgaggatgat *F 268100 *F Query: 181 gggaaaactcgtttttcgttgcattcttcggccggcgtcatcga 224 *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268099 gggaaaactcgtttttcgttgcattcttcggccggcgtcatcga 268056 *F Score = 394 bits (205), Expect = e-108 Genome Map *F Identities = 258/271 (95%), Gaps = 7/271 (2%) *F Strand = Plus / Minus *F Query: 436 gtccggggcactcttgtctcccgggttccgttctacagacgaccttaaggagggggtctt 495 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 267838 gtccggggcactcttgtctcccgggttccgttctacagacgaccttaaggagggggtctt *F 267779 *F Query: 496 gggaggccagtcgggagactggagcaaaccacatttcgtattccgtanacctggcgaaat 555 *F ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||| *F Sbjct: 267778 gggaggccagtcgggagactggagcaaaccacatttcgtattccgtacacctggcgaaat *F 267719 *F Query: 556 attcgatnacatgcgattttcaacaaccagaggacattgtttctattgaaatataataaa 615 *F ||||||| |||||||||||||||||| |||| |||||||||||||||||||||||||||| *F Sbjct: 267718 attcgatcacatgcgattttcaacaagcaga-gacattgtttctattgaaatataataaa *F 267660 *F Query: 616 ataagtaaatatggtatgtaacangaaaaagtttaagctttgatgattgttttgttacaa 675 *F |||||||||||| |||||||||| |||||| | |||||||||||||||||||| |||| *F Sbjct: 267659 ataagtaaatat-gtatgtaacag--aaaagtat-agctttgatgattgttttgtaacaa *F 267604 *F Query: 676 nagagcacgtcttaagaagtttttattcaag 706 *F ||||||||||||||| |||||||||||||| *F Sbjct: 267603 \-agagcacgtcttaag-agtttttattcaag 267575 *F Score = 198 bits (103), Expect = 1e-49 Genome Map *F Identities = 103/103 (100%) *F Strand = Plus / Minus *F Query: 275 ttaaagaggttccattggcataagtccggctcgatgatgatcgcgatttgccttggcaca 334 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 267999 ttaaagaggttccattggcataagtccggctcgatgatgatcgcgatttgccttggcaca *F 267940 *F Query: 335 acgcaatgccaaagttgttgtgccggttgttgccttccagctc 377 *F ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 267939 acgcaatgccaaagttgttgtgccggttgttgccttccagctc 267897 *F \# *F anon-EST:Liang-1.34 = ? something new ? *F >anon-EST:Liang-1.34 *F aaaacaaaac aaaacaaaaa cgacaaagca taaaaactgt atgttggttc ttttattata *F ttatacttta tttctataaa tattattgta attgtactgt gtattatgat tttgctaacc *F aaaatccctt agtatgagat gctcatcgat atttcacccc catacacaaa atcaaatcaa *F ttcgaaccaa aaagaatact cggccatcca aacgaacaaa atttaccatt tattttgtac *F gcaattgtaa ctacttttat atgtatgtac atatgtatgt acattctcta ttttggtacg *F tccactgaaa caaaattata gtacgcctca tttgattttt caactgtctt gtgtttcata *F cgatttaatt taatgatatt gattatgcaa atatatatat aaatataaac atcatttcta *F ttgtaattaa aatgctgcag acaacaaaga cgaacgacaa aaactcatag aaaacacaan *F aaaaacatct gaggagaagc cggagcggaa gaactggaaa gaattctccg cggggagggt *F cactaccccc ctcgcgttct cctactccaa cactttctcc ctttacatcc aanttgggct *F tccgtttaat cacgggattc ctcgggtgtt taattggttt tccctttttt tccncgccaa *F ctttctcata aaaatcaaan gaatcaatca tcaagnccgc agatgggaga ag *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003527|gb|AE003527|arm:3L 16253079..16532982 *F estimated-cyto:72E1-73A10 gadfly-seqname:AE003527 *F seq_release:3 *F Length = 279904 *F Score = 987 bits (513), Expect = 0.0 Genome Map *F Identities = 564/589 (95%), Gaps = 4/589 (0%) *F Strand = Plus / Minus *F Query: 37 ctgtatgttggttcttttattatattatactttatttctataaatattattgtaattgta 96 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99748 ctgtatgttggttcttttattatattatactttatttctataaatattattgtaattgta 99689 *F Query: 97 ctgtgtattatgattttgctaaccaaaatcccttagtatgagatgctcatcgatatttca 156 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99688 ctgtgtattatgattttgctaaccaaaatcccttagtatgagatgctcatcgatatttca 99629 *F Query: 157 cccccatacacaaaatcaaatcaattcgaaccaaaaagaatactcggccatccaaacgaa 216 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99628 cccccatacacaaaatcaaatcaattcgaaccaaaaagaatactcggccatccaaacgaa 99569 *F Query: 217 caaaatttaccatttattttgtacgcaattgtaactacttttatatgtatgtacatatgt 276 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99568 caaaatttaccatttattttgtacgcaattgtaactacttttatatgtatgtacatatgt 99509 *F Query: 277 atgtacattctctattttggtacgtccactgaaacaaaattatagtacgcctcatttgat 336 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99508 atgtacattctctattttggtacgtccactgaaacaaaattatagtacgcctcatttgat 99449 *F Query: 337 ttttcaactgtcttgtgtttcatacgatttaatttaatgatattgattatgcaannnnnn 396 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99448 ttttcaactgtcttgtgtttcatacgatttaatttaatgatattgattatgcaaatatat 99389 *F Query: 397 nnnnnnnnnnnaacatcatttctattgtaattaaaatgctgcagacaacaaagacgaacg 456 *F ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99388 atataaatataaacatcatttctattgtaattaaaatgctgcagacaacaaagacgaacg 99329 *F Query: 457 acaaaaactcatagaaaacacaanaaaaacatctgaggagaagccggagcggaagaactg 516 *F ||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 99328 acaaaaactcatagaaaacacaaaaaaaacatctgaggagaagccggagcggaagaactg 99269 *F Query: 517 gaaagaattctccgcggggagggtcactacccccctcgcgttctcctactccaacacttt 576 *F | |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 99268 g-aagaattctccgcgggga-ggtcactacccccctcgcgttctcctactccaacacttt 99211 *F Query: 577 ctccctttacatccaanttgggcttccgtttaatcacgggattcctcgg 625 *F |||||||||||||| | |||| |||||||||| |||| ||||||||||| *F Sbjct: 99210 ctccctttacatcc-agttggccttccgtttattcac-ggattcctcgg 99164 *F \# *F anon-EST:Liang-1.38 == something new ? *F >anon-EST:Liang-1.38 *F aataactttt acaaaataca taccaaaaat acttaattaa atgcaagcag gtattttttc *F aaataaaccg aatattccaa aaacacgttg cccaaataca cctacgatta attctaaagc *F aaacaaaatg ctcatataca cgtagctact aatgtaataa aaagaacact aaacgaatcc *F aaaacttaaa tcaggtattc acattttttt cgtattactt agactaagat aaagcgagtc *F ggcgagccgc cagcctgact aaatgactaa aatcatcgtg agatgatttc aaagattttt *F caaagatata aggaggatgt aaggaggagc gagcgttgca agacccacat atgtaactat *F ttactaagcc acgttttata acaaacataa caacaaatcg atcgacgatg ctaaagcaaa *F cggaatgaac tcttatcaaa atacaatttt gngtaatact tttaaaacga caaatgcaaa *F naacaganng aaaaaaanaa acgaatgctg cggccgccga attccggtct ccctanagtg *F agtcgtantt aatttcgata agccaagctg cattaangaa atcgggncaa ngcgcggggg *F anaggcggtt tgcgtnattg gggcgctctt ccggnttnct ngctcantnn gactncnctg *F cngcttnggt cgttngggct ggcggggaag c *F Database: dmel_all_scaffolds_r310 *F gadfly|SEG:AE003841|gb|AE003841|arm:2R 2310945..2577370 *F estimated-cyto:43A2-43D3 gadfly-seqname:AE003841 *F seq_release:3 *F Length = 266426 *F Score = 898 bits (467), Expect = 0.0 Genome Map *F Identities = 476/485 (98%) *F Strand = Plus / Minus *F Query: 1 aataacttttacaaaatacataccaaaaatacttaattaaatgcaagcaggtattttttc 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230552 aataacttttacaaaatacataccaaaaatacttaattaaatgcaagcaggtattttttc *F 230493 *F Query: 61 aaataaaccgaatattccaaaaacacgttgcccaaatacacctacgattaattctaaagc 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230492 aaataaaccgaatattccaaaaacacgttgcccaaatacacctacgattaattctaaagc *F 230433 *F Query: 121 aaacaaaatgctcatatacacgtagctactaatgtaataaaaagaacactaaacgaatcc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230432 aaacaaaatgctcatatacacgtagctactaatgtaataaaaagaacactaaacgaatcc *F 230373 *F Query: 181 aaaacttaaatcaggtattcacannnnnnncgtattacttagactaagataaagcgagtc 240 *F ||||||||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 230372 aaaacttaaatcaggtattcacatttttttcgtattacttagactaagataaagcgagtc *F 230313 *F Query: 241 ggcgagccgccagcctgactaaatgactaaaatcatcgtgagatgatttcaaagattttt 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230312 ggcgagccgccagcctgactaaatgactaaaatcatcgtgagatgatttcaaagattttt *F 230253 *F Query: 301 caaagatataaggaggatgtaaggaggagcgagcgttgcaagacccacatatgtaactat 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230252 caaagatataaggaggatgtaaggaggagcgagcgttgcaagacccacatatgtaactat *F 230193 *F Query: 361 ttactaagccacgttttataacaaacataacaacaaatcgatcgacgatgctaaagcaaa 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 230192 ttactaagccacgttttataacaaacataacaacaaatcgatcgacgatgctaaagcaaa *F 230133 *F Query: 421 cggaatgaactcttatcaaaatacaattttgngtaatacttttaaaacgacaaatgcaaa 480 *F ||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 230132 cggaatgaactcttatcaaaatacaattttgagtaatacttttaaaacgacaaatgcaaa *F 230073 *F Query: 481 naaca 485 *F |||| *F Sbjct: 230072 aaaca 230068 *F \# *F anon-EST:Liang-1.39 = no sequence data available *F \# *F anon-EST:Liang-1.4 = CG7977 *F >anon-EST:Liang-1.4 *F ttttcatttc tttttctcnt aaaaatgnca cccaaaaagc caaccgaaaa atccgccaag *F cctggcnaca anaacccata gcagaanaag actgctgngg ctcccgctgc cggcaagaan *F gaggctgctc cctcggntgc caantcanct gccgctgcgc ccaagaaggc tgctgcccca *F gcggctaata aggctgctcc ggncgccaaa aanccagcga ccgcnggtgc tgcngccaat *F aagcccgcng ccgtnaagan gancgcggtc gccaaggcca atagcaagga tgccaaganc *F aaggtcctcn ccggcaanaa nccccagtcc gtgctggcca anctctctgc caaggctcgc *F gcggccgtca angccaaaaa cggcgttaag cccgtgacca agcccnccaa gggaacngcn *F aaggccaagg ntgtggctct gctgaacgcc aagaangtcc agaagaagat atcaanggcn *F ccttcngnac ccntgcccgc aagatncgca ccaacgtgca cttccgtcgg taaccaccct *F gaagctgccc atggagtccc aagtacccca agnaaatcgg tgcccaccan gntaaccgca *F tggaccgcgt taacatcatc aaagtaccac tgga *F Database: dmel_all_transcript_r320 *F >CG7977-RA type=mRNA; *F loc=3L:complement(1647819..1648364,1648438..1648850, *F 1649225..1649306); ID=RpL23a-RA; name=RpL23a-RA; *F db_xref='CG7977,FlyBase:FBgn0026372'; len=1041 *F Length = 1041 *F Genome Map *F Score = 850 bits (442), Expect = 0.0 *F Identities = 548/607 (90%), Gaps = 6/607 (0%) *F Strand = Plus / Plus *F Query: 1 ttttcatttctttttctcntaaaaatgncacccaaaaagccaaccgaaaaatccgccaag 60 *F |||||||||||||||||| || ||||| ||||||||||||||||||| |||||||||||| *F Sbjct: 28 ttttcatttctttttctcgtagaaatgccacccaaaaagccaaccgagaaatccgccaag 87 *F Query: 61 cctggcnacaanaacccatagcagaanaagactgctgnggctcccgctgccggcaagaan 120 *F |||||| |||| || ||| ||||||| |||||||||| ||||||||||||||||||||| *F Sbjct: 88 cctggcgacaagaagccagagcagaagaagactgctgcggctcccgctgccggcaagaag 147 *F Query: 121 gaggctgctccctcggntgccaantcanctgccgctgcgcccaagaaggctgctgcccca 180 *F |||||||||||||||| |||||| || |||||||||||||||||||||||||||||||| *F Sbjct: 148 gaggctgctccctcggctgccaagccagctgccgctgcgcccaagaaggctgctgcccca 207 *F Query: 181 gcggctaataaggctgctccggncgccaaaaanccagcgaccgcnggtgctgcngccaat 240 *F |||||||| ||||||||||||| |||||| || ||||||||||| |||||||| ||||| *F Sbjct: 208 gcggctaagaaggctgctccggccgccaagaagccagcgaccgccggtgctgctgccaag 267 *F Query: 241 aagcccgcngccgtnaagangancgcggtcgccaaggccaatagcaaggatgccaaganc 300 *F |||||||| ||||| |||| || ||||| |||||||||||| |||||||||||||||| *F Sbjct: 268 aagcccgcggccgtaaagacgaccgcggccgccaaggccaagagcaaggatgccaagaag 327 *F Query: 301 aaggtcctcnccggcaanaanccccagtccgtgctggccaanctctctgccaaggctcgc 360 *F ||||||||| ||||||| || |||||||||||||||||||| |||||||||||||||||| *F Sbjct: 328 aaggtcctcgccggcaagaagccccagtccgtgctggccaagctctctgccaaggctcgc 387 *F Query: 361 gcggccgtcaangccaaaaacggcgttaagcccgtgaccaagcccnccaagggaacngcn 420 *F ||||||| ||| ||||| || ||||| |||||||||||||||||| |||||||||| || *F Sbjct: 388 gcggccgccaaggccaagaagggcgtgaagcccgtgaccaagcccgccaagggaaccgcc 447 *F Query: 421 aaggccaaggntgtggctctgctgaacgccaagaangtccagaagaagat-atcaanggc 479 *F |||||||||| |||||||||||||||||||||||| |||||||||||||| ||||| ||| *F Sbjct: 448 aaggccaaggctgtggctctgctgaacgccaagaaggtccagaagaagatcatcaagggc 507 *F Query: 480 nccttcngnacccntgcccgcaagatncgcaccaacgtgcacttccgtcgg-taaccacc 538 *F ||||| | |||| |||||||||||| |||||||||||||||||||||||| ||||||| *F Sbjct: 508 gccttcggcacccgtgcccgcaagatccgcaccaacgtgcacttccgtcggccaaccacc 567 *F Query: 539 ctgaagctgcccatggagtcccaagtaccccaagnaaatcggtgcccaccangntaaccg 598 *F ||||||||||||| ||||||||||||||||| || |||||||||||||||| | ||||| *F Sbjct: 568 ctgaagctgccca-ggagtcccaagtacccc-aggaaatcggtgcccaccagg--aaccg 623 *F Query: 599 catggac 605 *F ||||||| *F Sbjct: 624 catggac 630 *F \# *F anon-EST:Liang-1.40 == ? not in genome *F >anon-EST:Liang-1.40 *F aaatntctnt nanatgggan tcgtttnatt cgnangnaan ggaattgg *F Database: na_all.dros *F 377,099 sequences; 626,972,236 total letters *F Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, *F GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) 2,262,611 *F sequences; 10,883,439,008 total letters *F If you have any problems or questions with the results of this search *F please refer to the BLAST FAQs *F No significant similarity found. For reasons why, click here. *F \# *F anon-EST:Liang-1.43 = CG5720 *F >anon-EST:Liang-1.43 *F aaaagcgatg aatatgatga ggatctgttg aacttgagcg cgagggagga gtctgtagac *F caggagccag aagaggatcc cccgaaattg gaggctccac cacgaaaagc taagagcccc *F attattttta acagacgcga gaagacaccc gaaaagcgtc caaaggctga accggagatc *F gattcccgcc aggaggaggc ggaggagaag agccccaaaa aagattcttc ggctgacaag *F gccaaggaag accggtctga aaggcgatcg gtgcatgacc gtctcggttc aaagggccaa *F acgccggcag atcgttcgca acgcaaccaa aaggagctct atgtgccgac ccatcggcga *F cggagcgaac ccgaagccac caaagagcgc agtcaaagag agcgcacccg agaacgacgc *F tccagccgcg atactctaag gacactaacc gtaatcaacg ccancgtcgt tcgcctaatc *F cgggagggtc caagtacaaa agcaacgcat tgtctcccaa gttattgttg gccgtttaca *F agccaccgga gcccgtcgga cgatgaagga tttggccgaa aaaaccaant caactccgtt *F aataaaggnc aaacccanga ncaaatnttt ctcccaaaaa naanaggctg caagaaaact *F cctggtgcga acaatngggg gnngcncaaa ngtttcaaaa tnctcgncaa aganttntga *F cncaaaaagg ggggaccang gatttnggca aanattnana aattc *F Database: dmel_all_transcript_r320 *F >CG5720-RA type=mRNA; *F loc=3R:complement(20131027..20131577,20131639..20131908, *F 20131971..20134220,20134275..20134500, *F 20134594..20134799); ID=CG5720-RA; name=CG5720-RA; *F db_xref='CG5720,FlyBase:FBgn0028471'; len=3503 *F Length = 3503 *F Genome Map *F Score = 975 bits (507), Expect = 0.0 *F Identities = 606/636 (95%), Gaps = 10/636 (1%) *F Strand = Plus / Plus *F Query: 2 aaagcgatgaatatgatgaggatctgttgaacttgagcgcgagggaggagtctgtagacc 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 859 aaagcgatgaatatgatgaggatctgttgaacttgagcgcgagggaggagtctgtagacc 918 *F Query: 62 aggagccagaagaggatcccccgaaattggaggctccaccacgaaaagctaagagcccca 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 919 aggagccagaagaggatcccccgaaattggaggctccaccacgaaaagctaagagcccca 978 *F Query: 122 ttatttttaacagacgcgagaagacacccgaaaagcgtccaaaggctgaaccggagatcg 181 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 979 ttatttttaacagacgcgagaagacacccgaaaagcgtccagaggctgaaccggagatcg 1038 *F Query: 182 attcccgccaggaggaggcggaggagaagagccccaaaaaagattcttcggctgacaagg 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1039 attcccgccaggaggaggcggaggagaagagccccaaaaaagattcttcggctgacaagg 1098 *F Query: 242 ccaaggaagaccggtctgaaaggcgatcggtgcatgaccgtctcggttcaaagggccaaa 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 1099 ccaaggaagaccggtctgaaaggcgatcggtgcatgaccgtctcggttcaaaggcccaaa 1158 *F Query: 302 cgccggcagatcgttcgcaacgcaaccaaaaggagctctatgtgccgacccatcggcgac 361 *F ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1159 cgccggcagatcgttcgcagcgcaaccaaaaggagctctatgtgccgacccatcggcgac 1218 *F Query: 362 ggagcgaacccgaagccaccaaagagcgcagtcaaagagagcgcacccgagaacgacgct 421 *F ||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1219 ggagcgaacccgaggccaccaaagagcgcagtcaaagagagcgcacccgagaacgacgct 1278 *F Query: 422 ccagccgcgata-ctctaaggacactaaccgtaatcaacgccancgtcgttcgcctaatc 480 *F |||||||||||| ||||| |||||||||||||||||| ||||| ||||||||||||| || *F Sbjct: 1279 ccagccgcgatacctctagggacactaaccgtaatcagcgccagcgtcgttcgcctagtc 1338 *F Query: 481 cgggagggtccaagtacaaaagcaacgcattgtctcccaagttattgttggccgtttaca 540 *F | ||||| ||||||||| ||||||||||||||||||||||||||||| |||||||| ||| *F Sbjct: 1339 c-ggaggctccaagtac-aaagcaacgcattgtctcccaagttattg-tggccgttaaca 1395 *F Query: 541 agccaccggagcccgtcggacgatgaaggatttggccgaaaaaaccaantcaactccgtt 600 *F ||||||||||| ||||||||||||| ||||| |||||| |||| | | ||||||||||| *F Sbjct: 1396 agccaccggag-ccgtcggacgatg-aggatatggccg--aaaagccagtcaactccgtt 1451 *F Query: 601 aataaaggncaaacccangancaaatntttctccca 636 *F || |||| | || ||| || ||||| ||||||||| *F Sbjct: 1452 \-attaaggtc-aagccaagaccaaatgtttctccca 1485 *F \# *F anon-EST:Liang-1.52 = CG3998 *F >anon-EST:Liang-1.52 *F actcgccagc agcgcattgg cgttttttca ttttttaaag tgtacttaag agttgttaat *F atttttcgca gtgctcggcc cgaccacaac aaacaataat agccgaataa tagtgtgagt *F gaaaatgcgg cgagctgcat tcggcgcaaa accaagtgtt ccgcagcacc caaagcccaa *F atcgaacaac tcgaaatcgg aatcaggcga agccatcatt agcgagacat ttgtatttgt *F ctgacccctc aacggtgtct gagaatctcg gagagtcgtc gtggagcggt gtggagagga *F gagagcagga gcagaaaagg gcccacaatc caagtccaag gaagcaccac tccgatactg *F caccgatatg gaaaccgagt cgatggctcc aagcagcagt gcggcggctg cagccaccaa *F gctcctggtg gagatnacca ccgacgggaa taccaagctg cactgcccgg tgtgcaacaa *F agngctggtc tccctaaccg gatatgtgaa agcacgttaa ggaagcacca gccgncgggc *F ggnttcnagt tgccgtcact gcgatgctcc ggttttgcca cgaaggaggg agctnaccca *F gcnatgcaaa ggnatnanca agggggtcac tgggggccgg taactgggnc agggagccaa *F aagccntttc ntttgttaaa aaattgcggg cngggntana agttncaagg aggnataacc *F nttngccact ggaaggncca aatttttggn gaagga *F Database: dmel_all_transcript_r320 *F >CG3998-RA type=mRNA; *F loc=2L:join(9750567..9750982,9751909..9752282, *F 9752350..9753728,9753794..9754606); ID=zf30C-RA; *F name=zf30C-RA; db_xref='CG3998,FlyBase:FBgn0022720'; *F len=2982 *F Length = 2982 *F Genome Map *F Score = 929 bits (483), Expect = 0.0 *F Identities = 574/612 (93%), Gaps = 7/612 (1%) *F Strand = Plus / Plus *F Query: 1 actcgccagcagcgcattggcgnnnnnnnnnnnnnnaaagtgtacttaagagttgttaat 60 *F ||||||||||||| |||||||| |||||||||||||||||||||||| *F Sbjct: 46 actcgccagcagctcattggcgttttttcattttttaaagtgtacttaagagttgttaat 105 *F Query: 61 atttttcgcagtgctcggcccgaccacaacaaacaataatagccgaataatagtgtgagt 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 106 atttttcgcagtgctcggcccgaccacaacaaacaataatagccgaataatagtgtgagt 165 *F Query: 121 gaaaatgcggcgagctgcattcggcgcaaaaccaagtgttccgcagcacccaaagcccaa 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 166 gaaaatgcggcgagctgcattcggcgcaaaaccaagtgttccgcagcacccaaagcccaa 225 *F Query: 181 atcgaacaactcgaaatcggaatcaggcgaagccatcattagcgagacatttgtatttgt 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 226 atcgaacaactcgaaatcggaatcaggcgaagccatcattagcgagacatttgtatttgt 285 *F Query: 241 ctgacccctcaacggtgtctgagaatctcggagagtcgtcgtggagcggtgtggagagga 300 *F ||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 286 ctgacccctcagcggtgtctgagaatctcggagagtcgtcgtggagcggtgtggagagga 345 *F Query: 301 gagagcaggagcagaaaagggcccacaatccaagtccaaggaagcaccactccgatactg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | *F Sbjct: 346 gagagcaggagcagaaaagggcccacaatccaagtccaaggaagcaccactccgataccg 405 *F Query: 361 caccgatatggaaaccgagtcgatggctccaagcagcagtgcggcggctgcagccaccaa 420 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 406 aaccgatatggaaaccgagtcgatggctccaagcagcagtgcggcggctgcagccaccaa 465 *F Query: 421 gctcctggtggagatnaccaccgacgggaataccaagctgcactgcccggtgtgcaacaa 480 *F ||||||||||||||| ||||||||||| | |||||||||||||||||||||||||||| *F Sbjct: 466 gctcctggtggagatcaccaccgacggaatacccaagctgcactgcccggtgtgcaacaa 525 *F Query: 481 agngctggtctccctaaccggatatgtgaaagcacgttaaggaagcaccagccgncgggc 540 *F | ||||||||||||| ||||||||||| ||||||| ||| ||||||||||||| ||||| *F Sbjct: 526 ggcgctggtctccctagccggatatgtg-aagcacg-taaagaagcaccagccgccgggc 583 *F Query: 541 ggnttcnagttgccgtcactgcgatgctccggttttgccacgaaggagggagctnaccca 600 *F || ||| || |||||||||||||||||| ||||||||||||| |||| |||||| ||||| *F Sbjct: 584 ggcttcgag-tgccgtcactgcgatgct-cggttttgccacg-agga-ggagctcaccca 639 *F Query: 601 gcnatgcaaagg 612 *F || ||||||||| *F Sbjct: 640 gc-atgcaaagg 650 *F \# *F anon-EST:Liang-1.53 = looks like bacterial plasmid seq to me *F >anon-EST:Liang-1.53 *F aaaaaaaaaa aacgaatgct gcggccgccg aattccggtc tccctatagt gagtcgtatt *F aatttcgata agccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg *F tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg *F gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa *F cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc *F gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc *F aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag *F ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct *F cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta *F ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttnagcccg accgctgcgc *F cttanccggt aactancgtc ttgagtccaa cccggttaag acacgactta tcgccactgg *F gcagcagcca atggtaacan ggattaagca naaccnaggt aattnaggcg ggtgctanan *F aanttcttga antgggtggc ctaactac *F Database: na_all.dros *F >gi||gb|X01803|ATPG418 Transposable P vector conferring G418 *F resistance in Drosophila. (06-JUL-2002) *F Length = 5073 *F Score = 1104 bits (574), Expect = 0.0 *F Identities = 599/608 (98%), Gaps = 2/608 (0%) *F Strand = Plus / Plus *F Query: 72 gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct 131 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 911 gccagctggattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgct 970 *F Query: 132 cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtat 191 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 971 cttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtat 1030 *F Query: 192 cagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaaga 251 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1031 cagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaaga 1090 *F Query: 252 acatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgt 311 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1091 acatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgt 1150 *F Query: 312 ttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggt 371 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1151 ttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggt 1210 *F Query: 372 ggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc 431 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1211 ggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc 1270 *F Query: 432 gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaa 491 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1271 gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaa 1330 *F Query: 492 gcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgct 551 *F |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1331 gcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgct 1390 *F Query: 552 ccaagctgggctgtgtgcacgaaccccccgttnagcccgaccgctgcgccttanccggta 611 *F |||||||||||||||||||||||||||||||| |||||||||||||||||||| |||||| *F Sbjct: 1391 ccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggta 1450 *F Query: 612 actancgtcttgagtccaacccggttaagacacgacttatcgccactgggcagcagccaa 671 *F |||| ||||||||||||||||||| |||||||||||||||||||||| ||||||||||| *F Sbjct: 1451 actatcgtcttgagtccaacccgg-taagacacgacttatcgccact-ggcagcagccac 1508 *F Query: 672 tggtaaca 679 *F |||||||| *F Sbjct: 1509 tggtaaca 1516 *F Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, *F GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) 2,262,611 *F sequences; 10,883,439,008 total letters *F Score E *F Sequences producing significant alignments: (bits) Value *F gi|31414875|gb|AY260554.1| Retrotransposon vector MEL/ELM, ... 1233 0.0 *F gi|31414872|gb|AY260553.1| Retrotransposon vector ELM 5, co... 1233 0.0 *F gi|58199|emb|X65302.1|CVPGEM3 Cloning vector pGEM-3 1233 0.0 *F gi|58196|emb|X65300.1|CVPGEM1 Cloning vector pGEM-1 1233 0.0 *F gi|3142442|gb|AF062998.1|AF062998 Retrotransposon vector pV... 1233 0.0 *F gi|3142439|gb|AF062997.1|AF062997 Retrotransposon vector pV... 1233 0.0 *F gi|3142436|gb|AF062996.1|AF062996 Retrotransposon vector pV... 1233 0.0 *F gi|310816|gb|L08951.1|SYNPT7T318 pT7T318 cloning vector 1233 0.0 *F gi|310773|gb|L08872.1|SYNPGEM3V pGEM3 cloning vector Gemini... 1233 0.0 *F gi|310771|gb|L08870.1|SYNPGEM1V pGEM1 cloning vector Gemini... 1233 0.0 *F gi|58197|emb|X65301.1|CVPGEM2 Cloning vector pGEM-2 1225 0.0 *F gi|310772|gb|L08871.1|SYNPGEM2V pGEM2 cloning vector Gemini... 1225 0.0 *F gi|58205|emb|X65303.1|CVPGEM4 Cloning vector pGEM-4 1225 0.0 *F gi|310845|gb|L08948.1|SYNSP6T719 pSP6T719 cloning vector 1217 0.0 *F gi|310817|gb|L08952.1|SYNPT7T319 pT7T319 cloning vector 1217 0.0 *F gi|34105724|gb|AY336796.1| Transformation vector pICon, com... 1148 0.0 *F gi|32140408|gb|AY301066.1| Transposition vector RescueMu, c... 1148 0.0 *F gi|31747150|gb|AY279346.1| Gene silencing vector pMTCbLCVA.... 1148 0.0 *F gi|31747149|gb|AY279345.1| Gene silencing vector pCPCbLCVA.... 1148 0.0 *F gi|31747148|gb|AY279344.1| Gene silencing vector pCPCbLCVB.... 1148 0.0 *F gi|31580803|gb|AY297714.1| Yeast truncation assay backbone ... 1148 0.0 *F gi|20454202|gb|AF502128.1| Transient expression vector pBI2... 1148 0.0 *F gi|23506937|gb|AY157312.1| Cloning vector YDp-W, complete s... 1148 0.0 *F gi|23506935|gb|AY157311.1| Cloning vector YDp-U, complete s... 1148 0.0 *F gi|23506933|gb|AY157310.1| Cloning vector YDp-L, complete s... 1148 0.0 *F gi|23506931|gb|AY157309.1| Cloning vector YDp-K, complete s... 1148 0.0 *F gi|23506929|gb|AY157308.1| Cloning vector YDp-H, complete s... 1148 0.0 *F gi|22347652|gb|AF531173.1| Cloning vector pDblet, complete ... 1148 0.0 *F gi|47420069|gb|AY603762.1| Cloning vector pLucFXR, complete... 1148 0.0 *F gi|47420067|gb|AY603761.1| Cloning vector pLucLRH-1, comple... 1148 0.0 *F etc etc *F \# *F anon-EST:Liang-1.56 = CG7808 *F >anon-EST:Liang-1.56 *F ttttctcgct ttttactcga acatgggtat tagccgcgat agtgcacaca aacgccgggc *F caccggaggc aagcgcaagt cgctccgcaa gaagcgcaag ttcgagttgg gacgccccgc *F cgccaacacc aagcttggct ccggccgcgt gcacaaggtg cgcacccgtg gtggaaacac *F caagctccgt gctctgcgcc tggaaaccgg aaacttcgcc tgggcctccg agggagtggc *F gcgcaagacc cgtatcgccg atgttgtgta caacgcctcc aacaacgagc tggtgcgaac *F caagaccttg gtgaagaaca gcatcgtggt catcgatgcc acgcccttcc gccagtggta *F cgaggctcac tacgtgctgc ccctgggacg caagcgtaac cccaagcacg cccagaagga *F ggacgagaac gacgtgctga ccaagaagcg cagcgaaaag gttatgaaga agtactggag *F cgccaaaagt acggcaaggt cgaacaaggc ctcgaggata attcaactcc gggcgcatcn *F tgggttgcat ttcttccgcc ccgganatnc ggtcgctccg aangggtaaa atttggaang *F naaaggantt ggaattcaac ntaagaagnn taagnttaaa gaaataaggn actanataaa *F ccttgaaaga cattgatttt gaaacaaaaa acngggataa aanaacttgg ttttttaaag *F gaagtgcatt *F Database: dmel_all_transcript_r320 *F >CG7808-RC type=mRNA; *F loc=3R:join(25676955..25677022,25677287..25677393, *F 25677873..25678284,25678503..25678717); ID=CG7808-RC; *F name=CG7808-RC; db_xref='CG7808,FlyBase:FBgn0039713'; *F len=802 *F Length = 802 *F Genome Map *F Score = 1035 bits (538), Expect = 0.0 *F Identities = 682/734 (92%), Gaps = 12/734 (1%) *F Strand = Plus / Plus *F Query: 2 tttctcgctttttactcgaacatgggtattagccgcgatagtgcacacaaacgccgggcc 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 44 tttctcgctttttactcgaacatgggtattagccgcgatagtgcacacaaacgccgggcc 103 *F Query: 62 accggaggcaagcgcaagtcgctccgcaagaagcgcaagttcgagttgggacgccccgcc 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 104 accggaggcaagcgcaagtcgctccgcaagaagcgcaagttcgagttgggacgccccgcc 163 *F Query: 122 gccaacaccaagcttggctccggccgcgtgcacaaggtgcgcacccgtggtggaaacacc 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 164 gccaacaccaagcttggctccggccgcgtgcacaaggtgcgcacccgtggtggaaacacc 223 *F Query: 182 aagctccgtgctctgcgcctggaaaccggaaacttcgcctgggcctccgagggagtggcg 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 224 aagctccgtgctctgcgcctggaaaccggaaacttcgcctgggcctccgagggagtggcg 283 *F Query: 242 cgcaagacccgtatcgccgatgttgtgtacaacgcctccaacaacgagctggtgcgaacc 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 284 cgcaagacccgtatcgccgatgttgtgtacaacgcctccaacaacgagctggtgcgaacc 343 *F Query: 302 aagaccttggtgaagaacagcatcgtggtcatcgatgccacgcccttccgccagtggtac 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 344 aagaccttggtgaagaacagcatcgtggtcatcgatgccacgcccttccgccagtggtac 403 *F Query: 362 gaggctcactacgtgctgcccctgggacgcaagcgtaaccccaagcacgcccagaaggag 421 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 404 gaggctcactacgtgctgcccctgggacgcaagcgtaaccccaagcacgcccagaaagag 463 *F Query: 422 gacgagaacgacgtgctgaccaagaagcgcagcgaaaaggttatgaagaagta-ctggag 480 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| *F Sbjct: 464 gacgagaacgacgtgctgaccaagaagcgcagcgaaaaggttatgaagaagtacctggag 523 *F Query: 481 cgccaaaagtacggcaaggtcgaacaaggcctcgaggat-aattcaactccgggcgcatc 539 *F ||||| ||||||||||||||||| || | |||||||||| | |||| |||||| |||||| *F Sbjct: 524 cgccagaagtacggcaaggtcgagcaggccctcgaggatcagttcacctccggccgcatc 583 *F Query: 540 ntgggttgcatttctt-ccgccccggana-tncggtcgctccgaangggtaaaatttgga 597 *F ||| ||||||||||| |||||||||| | | ||||||||||| | || || | |||||| *F Sbjct: 584 ttggcttgcatttcttcccgccccggacagtgcggtcgctccg-acggctacattttgga 642 *F Query: 598 angnaaagganttggaattc-aacntaagaagnntaagnttaaagaaataaggnactana 656 *F | | ||||| ||||||||| | | ||||||| ||| ||| || |||| |||| | *F Sbjct: 643 aggc-aaggagttggaattctaccttaagaagatcaagtctaagaaataaaggcactaga 701 *F Query: 657 taaaccttgaaagacattgattttgaaacaaaaaacngggataaaanaacttggtttttt 716 *F | || |||| |||||| |||||| ||||||||| || |||||| ||||| ||||||| *F Sbjct: 702 t-aagcttgcaagaca-tgatttgcaaacaaaaa--cggaataaaacaactt-gtttttt 756 *F Query: 717 aaaggaagtgcatt 730 *F | |||||||||||| *F Sbjct: 757 ataggaagtgcatt 770 *F \# *F anon-EST:Liang-1.62 no sequence data available *F \# *F anon-EST:Liang-1.66 = CG4978 *F >anon-EST:Liang-1.66 *F gaccaggtgc ccgtgggcca cattccgcgc agcatgacca tcatgtgcag gggtgaggtc *F actcgaatgg cccagcctgg ggatcacatt gtggtctctg gagtgttctt gccactgatg *F cgcacaggtt tcgctcagat gattcagggt ttgctctcgg agacattcct tcaagctcac *F cgcatcatct gtatcaacaa gaacgacgag atttcggaca aggatgccga gctgactcca *F gaagagctgg aggaattggc ccaggatgac ttctacgagc gcttggccac cagcttggca *F cccgaaatct atggccattt ggatgttaag aaagcgctgc tcttgctgtt ggtcggagga *F gttgacaagc ggcccgatgg catgaagatt cgtggcaaca tcaacatctg cctgatggga *F gatcccggtg tggccaagtc gcaactgctg ggctacatta gccgattggc cgtgcgatcg *F cagtacacca caggacgaag ttcttcgggg gtgggtctca cgctgcggtc atgaaggatc *F ccctcacagg cgaaatgact tggaaggaag actcttgtgc tggctgatca gggatctgct *F gcattgataa ttcacaaatg gcggatcagg atcttacacc ttcatagtga tggaacacaa *F accatttcat accaaaagca ggctctacaa cctaaacctc ctttcaatcc tgggccgctg *F ctaatccccc ttttggaacc taccatcctc cttcctacc *F Database: dmel_all_transcript_r320 *F >CG4978-RA type=mRNA; *F loc=3L:join(8850320..8850489,8850554..8850923, *F 8850995..8851496,8851564..8852621,8852684..8853045); *F ID=Mcm7-RA; name=Mcm7-RA; *F db_xref='CG4978,FlyBase:FBgn0020633'; len=2462 *F Length = 2462 *F Genome Map *F Score = 1063 bits (553), Expect = 0.0 *F Identities = 626/645 (97%), Gaps = 7/645 (1%) *F Strand = Plus / Plus *F Query: 1 gaccaggtgcccgtgggccacattccgcgcagcatgaccatcatgtgcaggggtgaggtc 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 863 gaccaggtgcccgtgggccacattccgcgcagcatgaccatcatgtgcaggggtgaggtc 922 *F Query: 61 actcgaatggcccagcctggggatcacattgtggtctctggagtgttcttgccactgatg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 923 actcgaatggcccagcctggggatcacattgtggtctctggagtgttcttgccactaatg 982 *F Query: 121 cgcacaggtttcgctcagatgattcagggtttgctctcggagacattccttcaagctcac 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 983 cgcacaggtttcgctcagatgattcagggtttgctctcggagacattccttcaagctcac 1042 *F Query: 181 cgcatcatctgtatcaacaagaacgacgagatttcggacaaggatgccgagctgactcca 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1043 cgcatcatctgtatcaacaagaacgacgagatttcggacaaggatgccgagctgactcca 1102 *F Query: 241 gaagagctggaggaattggcccaggatgacttctacgagcgcttggccaccagcttggca 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1103 gaagagctggaggaattggcccaggatgacttctacgagcgcttggccaccagcttggca 1162 *F Query: 301 cccgaaatctatggccatttggatgttaagaaagcgctgctcttgctgttggtcggagga 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1163 cccgaaatctatggccatttggatgttaagaaagcgctgctcttgctgttggtcggagga 1222 *F Query: 361 gttgacaagcggcccgatggcatgaagattcgtggcaacatcaacatctgcctgatggga 420 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 1223 gttgacaagcggcccgatggcatgaagattcgtggcaacattaacatctgcctgatggga 1282 *F Query: 421 gatcccggtgtggccaagtcgcaactgctgggctacattagccgattggccgtgcgatcg 480 *F ||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 1283 gatcccggtgtggccaagtcgcagctgctgggctacattagccgattggccgtgcgatcg 1342 *F Query: 481 cagtacaccacaggacgaagttcttcgggggtgggtctca-cgctgcggtcatgaaggat 539 *F |||||||||||||| ||| ||||||||||||||||||| | |||||||||||||||||| *F Sbjct: 1343 cagtacaccacagggcgaggttcttcgggggtgggtcttaccgctgcggtcatgaaggac 1402 *F Query: 540 cccctcacaggcgaaatgac-ttggaaggaaga-ctcttgtgctggctgatcaggga-tc 596 *F || ||||||||||| ||||| ||||||||| || ||||||||||||||||||||||| || *F Sbjct: 1403 cctctcacaggcgagatgacattggaaggaggagctcttgtgctggctgatcagggagtc 1462 *F Query: 597 tgctgcattgat-aattc-acaa-atggcggatcaggatcttaca 638 *F |||||||||||| | ||| |||| |||||||||||||||| |||| *F Sbjct: 1463 tgctgcattgatgagttcgacaagatggcggatcaggatcgtaca 1507 *F \# *F anon-EST:Liang-1.72 = CG12011 *F >anon-EST:Liang-1.72 *F acagttcaac tgaaaccctc atcatgcatt ctctacgact cctggttctt cttggtgttc *F tgatggccac tcagggcaga gtcctagaac aggacaagac tccggaggtt gtcggttcaa *F ctcaagaaat caaaccaaac gtggaggtca agcaggaagt tgccaataaa gtgccggaaa *F cccagccgaa gctgggtggc gctgtcatct cccaaggcca atcttctgca gctatcgatc *F aggttggtgt gaaggagggt caagatcagg tggcgaactc cacaactcct gatgtccagg *F aaagccaacg cgaaggggtt tctttcgtcg aagtgactaa agaaaaccag gaagcacatg *F ccccactgag taaggtcgtc agtagccagt agccccagcg atagccgagg aaaagcagcc *F cacttccaat gagataccca aggaccaagg agccacgcaa agaagttata acccagaact *F atcccgacgt ccagggacgc gacaataaga ttcagttggt gttcgagcaa cctggtcaaa *F accaaaacca agattccaca gataagcatt caggatttgg gagaaagatt ttcaagtatc *F anggagtgca aattggtcaa cgggatntca atcaatttaa accagtatcc gagcatctac *F aaacaanntt ccattcttta cccgaaaggg tggaaaaant tttctgtggg taaaaaaaac *F cggatgccaa t *F Database: dmel_all_transcript_r320 *F >CG12011-RA type=mRNA; *F loc=3L:join(1675248..1675273,1675346..1677049); Genome Map *F ID=CG12011-RA; name=CG12011-RA; *F db_xref='CG12011,FlyBase:FBgn0035257'; len=1730 *F Length = 1730 *F Score = 1035 bits (538), Expect = 0.0 *F Identities = 634/663 (95%), Gaps = 9/663 (1%) *F Strand = Plus / Plus *F Query: 1 acagttcaactgaaaccctcatcatgcattctctacgactcctggttcttcttggtgttc 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 acagttcaactgaaaccctcatcatgcattctctacgactcctggttcttcttggtgttc 60 *F Query: 61 tgatggccactcagggcagagtcctagaacaggacaagactccggaggttgtcggttcaa 120 *F |||||||||||||||||||||| ||||||||||||||| ||||||||||||||| |||| *F Sbjct: 61 tgatggccactcagggcagagttctagaacaggacaaggctccggaggttgtcgcttcac 120 *F Query: 121 ctcaagaaatcaaaccaaacgtggaggtcaagcaggaagttgccaataaagtgccggaaa 180 *F |||||||||||||||| ||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 121 ctcaagaaatcaaacccaacgtggaggtcaagcaggaagttgccaataaagtgcgggaaa 180 *F Query: 181 cccagccgaagctgggtggcgctgtcatctcccaaggccaatcttctgcagctatcgatc 240 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 cccagccgcagctgggtggcgctgtcatctcccaaggccaatcttctgcagctatcgatc 240 *F Query: 241 aggttggtgtgaaggagggtcaagatcaggtggcgaactccacaactcctgatgtccagg 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 aggttggtgtgaaggagggtcaagatcaggtggcgaactccacaactcctgatgtccagg 300 *F Query: 301 aaagccaacgcgaaggggtttctttcgtcgaagtgactaaagaaaaccaggaagcacatg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301 aaagccaacgcgaaggggtttctttcgtcgaagtgactaaagaaaaccaggaagcacatg 360 *F Query: 361 ccccactgagtaaggtcgtcagtag-ccagtagccccagcgatagccgaggaaaagcagc 419 *F ||||||||||||||||||||||||| ||| |||||||||||||||||||||||||||||| *F Sbjct: 361 ccccactgagtaaggtcgtcagtagcccattagccccagcgatagccgaggaaaagcagc 420 *F Query: 420 ccacttccaatgagatacccaaggaccaaggagccacgcaaagaagttataacccagaac 479 *F ||||||||||||||||||||||||||| |||||||| ||||||||||||||||||||||| *F Sbjct: 421 ccacttccaatgagatacccaaggacc-aggagccaggcaaagaagttataacccagaac 479 *F Query: 480 tatcccgacgtccagggacgcgacaataagattcagttggtgttcgagcaacctggtcaa 539 *F ||||||||||||| ||||||||||||||||||||||| |||| |||||| ||||||||| *F Sbjct: 480 catcccgacgtccaaggacgcgacaataagattcagtttgtgtacgagcagcctggtcaa 539 *F Query: 540 aaccaaaaccaagattccacagataagcattcaggatttgggagaaagattttcaagtat 599 *F |||||||||| |||||||||||||||||||||||||| | |||| ||||| ||||||||| *F Sbjct: 540 aaccaaaacc-agattccacagataagcattcaggatct-ggag-aagatcttcaagtat 596 *F Query: 600 canggagtgcaaattggtcaacgggatntcaatcaatttaaaccagtatccgagcatcta 659 *F || ||||||| || |||||||| |||| ||||||| || |||||||||||||||||||| *F Sbjct: 597 cagggagtgc-aagtggtcaac-ggatctcaatca--tttaaccagtatccgagcatcta 652 *F Query: 660 caa 662 *F ||| *F Sbjct: 653 caa 655 *F \# *F anon-EST:Liang-1.74 = CG14938 *F >anon-EST:Liang-1.74 *F ttttctacat ttttgaaatg tgtgccaggc ggcgcgtgac tttgtttggg tcgatcgata *F tggtttttgt gatatccgat tgttccttag ctgcgctcag tgaatcaaat ggatccgaag *F accaatccgt ctcaggcaat tttctcgcta gacacttaaa gttaaggggt ttttgttgcc *F gtaaccagca gaagcgcacc atcttgtatt tttattgtgt tttgactaag ttatgattat *F atccatatgg agcgacattc atagaaagca tcctcatcat acataaacat atgcagatat *F gcataggcat acaattataa tatatactta tagtttgtaa ataacacgca acaagaccct *F tggaaatcta tcgaaaatta ttatcacaac tgtattattt ttgcattttc caaggaggaa *F cattaataac gagctaacgc gcgaaaactt tcacatatat tggccaagtg tagtccttaa *F cccagtttac aatatatcga gccctgaaag tggggctgtg atgattatga gatatatata *F caattatatg acagtggaaa cagaagcagc ataacggnag acccaactaa cgaaaataat *F gaaaagggat taaacaaaca agttattana tttaatgtta ggttgaacta ctaaaagnta *F atgattgaaa agcacggggt ntaaagggat ggngaanagg agttcccgcn tgggaatgcg *F aatagntttt anttggggcg c *F Database: dmel_all_transcript_r320 *F >CG14938-RD type=mRNA; *F loc=2L:complement(11783198..11786612,11786700..11786912, *F 11787536..11788021,11788106..11788273,11791158..11792545, *F 11792656..11792769,11794919..11795028, *F 11797997..11798304); ID=crol-RD; name=crol-RD; *F db_xref='CG14938,FlyBase:FBgn0020309'; len=6202 *F Length = 6202 *F Genome Map *F Score = 1248 bits (649), Expect = 0.0 *F Identities = 715/737 (97%), Gaps = 7/737 (0%) *F Strand = Plus / Plus *F Query: 1 ttttctacatttttgaaatgtgtgccaggcggcgcgtgactttgtttgggtcgatcgata 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4076 ttttctacatttttgaaatgtgtgccaggcggcgcgtgactttgtttgggtcgatcgata 4135 *F Query: 61 tggtttttgtgatatccgattgttccttagctgcgctcagtgaatcaaatggatccgaag 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4136 tggtttttgtgatatccgattgttccttagctgcgctcagtgaatcaaatggatccgaag 4195 *F Query: 121 accaatccgtctcaggcaattttctcgctagacacttaaagttaaggggtttttgttgcc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4196 accaatccgtctcaggcaattttctcgctagacacttaaagttaaggggtttttgttgcc 4255 *F Query: 181 gtaaccagcagaagcgcaccatcttgtatttttattgtgttttgactaagttatgattat 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4256 gtaaccagcagaagcgcaccatcttgtatttttattgtgttttgactaagttatgattat 4315 *F Query: 241 atccatatggagcgacattcatagaaagcatcctcatcatacataaacatatgcagatat 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4316 atccatatggagcgacattcatagaaagcatcctcatcatacataaacatatgcagatat 4375 *F Query: 301 gcataggcatacaattataatatatacttatagtttgtaaataacacgcaacaagaccct 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4376 gcataggcatacaattataatatatacttatagtttgtaaataacacgcaacaagaccct 4435 *F Query: 361 tggaaatctatcgaaaattattatcacaactgtattatttttgcattttccaaggaggaa 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4436 tggaaatctatcgaaaattattatcacaactgtattatttttgcattttccaaggaggaa 4495 *F Query: 421 cattaataacgagctaacgcgcgaaaactttcacatatattggccaagtgtagtccttaa 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4496 cattaataacgagctaacgcgcgaaaactttcacatatattggccaagtgtagtccttaa 4555 *F Query: 481 cccagtttacaatatatcgagccctgaaagtggggctgtgatgattatgagatatatata 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4556 cccagtttacaatatatcgagccctgaaagtggggctgtgatgattatgagatatatata 4615 *F Query: 541 caattatatgacagtggaaacagaagcagcataacggnagacccaactaacgaaaataat 600 *F ||||||||||||||||||||||||||||||||||||| ||||||||||||||| | |||| *F Sbjct: 4616 caattatatgacagtggaaacagaagcagcataacggcagacccaactaacgaga-taat 4674 *F Query: 601 gaaaagggattaaacaaacaagttattanatttaatgttaggttgaactactaaaagnta 660 *F |||||||||||||||||||||||||||| |||||||||||| ||||||||||||||| || *F Sbjct: 4675 gaaaagggattaaacaaacaagttattatatttaatgttag-ttgaactactaaaag-ta 4732 *F Query: 661 atgattgaaaagcacggggtntaaagggatggngaanaggagttcccgcntgggaatgcg 720 *F |||||||||| |||||| |||| |||| || |||||||||||| ||||| |||| *F Sbjct: 4733 atgattgaaa-gcacggatcg--aaggaatggaga-taggagttcccgcgtgggagtgcg 4788 *F Query: 721 aatagnttttanttggg 737 *F | ||| ||||| ||||| *F Sbjct: 4789 agtagtttttagttggg 4805 *F \# *F anon-EST:Liang-1.75 = CG5481 *F >anon-EST:Liang-1.75 *F agcaacaggc gcagcagccg caccagcaac accaggctct ccagcagcac cagcaactgc *F cacccagcaa catctaccag cagatgtcca ccaccagcga gatatacccc acgaacacgg *F gtccttcgcg ctctgtctac tctgagcagt attactaccc caaggacaag cagagacaca *F tccacatcac cgagaacaag ctgagcaact gccacaccta tgaggcggct cctggcgcca *F agcagtcctc gccgatatcc tcgcagttcg ccagcgtgag gcggcagcag ctgccgccca *F actgcagcat cggcagggaa agtgcccgct tcaaggtgct aaacacggat cagggcaaga *F accagcagaa tctcctggat ctcgacggct cctcgatgtg ctacaacggt ctggcagact *F cgggctgcgg tggatctccc tccccgatgg ccatgctgat gtcgcacgag gacgagcacg *F cgctgtacca cacggcggat ggggatctgg acgacatgga acgactgtac gtcaaggtgg *F acgagcagca gcctccgcag cagcagcagc agctgatacc ctagttccac agcatccggg *F cggaangtca cctgcagtcc tggngggaat cagagcacgc ggancagtcc ggaagaacgg *F caggaatgca tcaaggganc caacnagttg tntacctccg gggaancgtg nccaangaaa *F nggagcct *F Database: dmel_all_transcript_r320 *F >CG5481-RA type=mRNA; *F loc=2L:complement(1382554..1384298,1386593..1386878, *F 1388514..1388677,1388773..1389019,1389097..1389192, *F 1392559..1392964,1393055..1393174,1393253..1394190, *F 1394356..1394487,1395858..1395998,1396140..1396424, *F 1397569..1397671,1398728..1398918,1421570..1422115); *F ID=lea-RA; name=lea-RA; *F db_xref='CG5481,FlyBase:FBgn0002543'; len=5400 *F Length = 5400 *F Genome Map *F Score = 1037 bits (539), Expect = 0.0 *F Identities = 541/542 (99%) *F Strand = Plus / Plus *F Query: 1 agcaacaggcgcagcagccgcaccagcaacaccaggctctccagcagcaccagcaactgc 60 *F ||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3895 agcaacaggcgcagcagacgcaccagcaacaccaggctctccagcagcaccagcaactgc 3954 *F Query: 61 cacccagcaacatctaccagcagatgtccaccaccagcgagatataccccacgaacacgg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3955 cacccagcaacatctaccagcagatgtccaccaccagcgagatataccccacgaacacgg 4014 *F Query: 121 gtccttcgcgctctgtctactctgagcagtattactaccccaaggacaagcagagacaca 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4015 gtccttcgcgctctgtctactctgagcagtattactaccccaaggacaagcagagacaca 4074 *F Query: 181 tccacatcaccgagaacaagctgagcaactgccacacctatgaggcggctcctggcgcca 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4075 tccacatcaccgagaacaagctgagcaactgccacacctatgaggcggctcctggcgcca 4134 *F Query: 241 agcagtcctcgccgatatcctcgcagttcgccagcgtgaggcggcagcagctgccgccca 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4135 agcagtcctcgccgatatcctcgcagttcgccagcgtgaggcggcagcagctgccgccca 4194 *F Query: 301 actgcagcatcggcagggaaagtgcccgcttcaaggtgctaaacacggatcagggcaaga 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4195 actgcagcatcggcagggaaagtgcccgcttcaaggtgctaaacacggatcagggcaaga 4254 *F Query: 361 accagcagaatctcctggatctcgacggctcctcgatgtgctacaacggtctggcagact 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4255 accagcagaatctcctggatctcgacggctcctcgatgtgctacaacggtctggcagact 4314 *F Query: 421 cgggctgcggtggatctccctccccgatggccatgctgatgtcgcacgaggacgagcacg 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4315 cgggctgcggtggatctccctccccgatggccatgctgatgtcgcacgaggacgagcacg 4374 *F Query: 481 cgctgtaccacacggcggatggggatctggacgacatggaacgactgtacgtcaaggtgg 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4375 cgctgtaccacacggcggatggggatctggacgacatggaacgactgtacgtcaaggtgg 4434 *F Query: 541 ac 542 *F || *F Sbjct: 4435 ac 4436 *F \# *F anon-EST:Liang-1.76 = CG31363 *F >anon-EST:Liang-1.76 *F gacttaattc gcctttcgaa ctgaacgaac gtgccgcggc atctgcaatt ttccgtgcat *F ttccacttgc attttgtgtg tttcactttg ttacgtgtgt gccagttttc catttaatcg *F ttaccgatcg tccaataagc ggctacagcg gcaaaatgat ctctaacttt gattgcaccg *F ataatcaggc cagcagcaag gtgctgaggc ccccgggcgg cggatcgagc gacatctttg *F gatcggagat gccgcagacc cccaggaacg tgaagaatcg catggcgtcc aacatattcg *F ctgccgagaa agataatgga gtgaaaaaca acggtgatgc accacgtcgc ggccagaaga *F ccgtcgactc ccactctcgg ctgtttgggg agcccacccg cccgatcacc cccggcaaga *F accacatgaa gagcagcatt ccctttggtc agaacacaga ggccgttgcc gcccagaagc *F tgctgaccac caatggccac tacaacggca agagcggatc ggtgtcctcg gcctcgtctt *F cggtgtcgtc ctccancgag aacctcaaag atgaacagtg gctcgagatc aganggcaac *F cccgtcacan gcgaaggcta caaggtcgta accaacgagt attcccaagc gccaggaagt *F cgtccaatng cgggaactcc ggtgatnaac aaagaaacgg cattccccca ngcgggnaat *F cgtcgggnct gttgtaatga caanccggga attt *F Database: dmel_all_transcript_r320 *F >CG31363-RD type=mRNA; *F loc=3R:complement(7416128..7417047,7418107..7418364, *F 7419373..7419506,7445444..7445674); ID=CG31363-RD; *F name=CG31363-RD; db_xref='CG31363,FlyBase:FBgn0051363'; *F len=1543 *F Length = 1543 *F Genome Map *F Score = 1260 bits (655), Expect = 0.0 *F Identities = 718/741 (96%), Gaps = 6/741 (0%) *F Strand = Plus / Plus *F Query: 2 acttaattcgcctttcgaactgaacgaacgtgccgcggcatctgcaattttccgtgcatt 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| *F Sbjct: 34 acttaattcgcctttcgaactgaacgaacgtgccgcggcatctgcaattttctgtgcatt 93 *F Query: 62 tccacttgcattttgtgtgtttcactttgttacgtgtgtgccagttttccatttaatcgt 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 94 tccacttgcattttgtgtgtttcactttgttacgtgtgtgccagttttccatttaatcgt 153 *F Query: 122 taccgatcgtccaataagcggctacagcggcaaaatgatctctaactttgattgcaccga 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 154 taccgatcgtccaataagcggctacagcggcaaaatgatctctaactttgattgcaccga 213 *F Query: 182 taatcaggccagcagcaaggtgctgaggcccccgggcggcggatcgagcgacatctttgg 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 214 taatcaggccagcagcaaggtgctgaggcccccgggcggcggatcgagcgacatctttgg 273 *F Query: 242 atcggagatgccgcagacccccaggaacgtgaagaatcgcatggcgtccaacatattcgc 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 274 atcggagatgccgcagacccccaggaacgtgaagaatcgcatggcgtccaacatattcgc 333 *F Query: 302 tgccgagaaagataatggagtgaaaaacaacggtgatgcaccacgtcgcggccagaagac 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 334 tgccgagaaagataatggagtgaaaaacaacggtgatgcaccacgtcgcggccagaagac 393 *F Query: 362 cgtcgactcccactctcggctgtttggggagcccacccgcccgatcacccccggcaagaa 421 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 394 cgtcgactcccactctcggctgtttggggagcccacccgcccgatcacccccggcaagaa 453 *F Query: 422 ccacatgaagagcagcattccctttggtcagaacacagaggccgttgccgcccagaagct 481 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 454 ccacatgaagagcagcattccctttggtcagaacacagaggccgttgccgcccagaagct 513 *F Query: 482 gctgaccaccaatggccactacaacggcaagagcggatcggtgtcctcggcctcgtcttc 541 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 514 gctgaccaccaatggccactacaacggcaagagcggatcggtgtcctcggcctcgtcttc 573 *F Query: 542 ggtgtcgtcctccancgagaacctcaaagatgaacagtggctcgagatcaganggcaacc 601 *F |||||||||||||| |||||||||| |||||||||||||||||||||||||| ||||||| *F Sbjct: 574 ggtgtcgtcctccaccgagaacctc-aagatgaacagtggctcgagatcagagggcaacc 632 *F Query: 602 ccgtcacangcgaaggctacaaggtcgtaaccaacgagtattcccaagcgccaggaagtc 661 *F |||||||| |||| ||||||||||||||| ||||||||||||||| ||||||||| |||| *F Sbjct: 633 ccgtcacaggcgagggctacaaggtcgtagccaacgagtattccc-agcgccagg-agtc 690 *F Query: 662 gtccaatngcgggaactccggtgatnaacaaagaaacggcattcccccangcgggnaatc 721 *F ||||||| || || ||||||||||| ||||| ||| ||||||||||| |||| | || *F Sbjct: 691 gtccaatggc-ggcactccggtgatcaacaa--gaaccgcattcccccaggcggctactc 747 *F Query: 722 gtcgggnctgttgtaatgaca 742 *F |||||| |||| ||||||||| *F Sbjct: 748 gtcgggcctgtggtaatgaca 768 *F \# *F anon-EST:Liang-1.79 = CG5395 *F >anon-EST:Liang-1.79 *F ggctctaaat agaatatctg cagaaacatg gacaacttcg gactgggcgg atcggatcta *F agcaaggggc agatattcca ggtactggtt cgcctgtctg tggcctcgct gatcacatat *F tattcggtga aatggatgat gaaccagatg gatccgacca gtaaaaacaa gaaaaaggcc *F aaagttctgg ctgaggagca gcttaagagg ctagccgagc aggagggctt caagctaaga *F gggcaagagt ttagcgacta cgagcttatg attgcgtcac atcttgttgt tcccgccgac *F ataacagtca gttgggccga tatcgccggt ctgggattca gtcattcatg agctccggga *F atcggtggtg ctgccaatcc agcacaagga tctcttcaag cattcgaagc tgtggcaggc *F gcccaaaggc gtcctgctcc atggacnanc gggctgtgga aagacgctga tancgaaggc *F gacggnaaaa gaagcgggaa tgcgccttta tcaacttggg angtcgccat tcttancgac *F aagtggtacg gggaatncca ngaaattgac ttctgctgtc ttctcgcttc atcncgaatt *F tagcatgcan taatttttat tgacnaaaat aactcatttt tgngatttcg anacattacg *F ancattaang gnnaccgccc attattaagn acccantttt atnatagcng tggggattgg *F cttagcacca aat *F Database: dmel_all_transcript_r320 *F >CG5395-RA type=mRNA; *F loc=2L:complement(10295001..10296007, *F 10296064..10296354); ID=nmd-RA; name=nmd-RA; *F db_xref='CG5395,FlyBase:FBgn0005322'; len=1298 *F Length = 1298 *F Genome Map *F Score = 1063 bits (553), Expect = 0.0 *F Identities = 630/657 (95%), Gaps = 7/657 (1%) *F Strand = Plus / Plus *F Query: 2 gctctaaatagaatatctgcagaaacatggacaacttcggactgggcggatcggatctaa 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 84 gctctaaatagaatatctgcagaaacatggacaacttcggactgggcggatcggatctaa 143 *F Query: 62 gcaaggggcagatattccaggtactggttcgcctgtctgtggcctcgctgatcacatatt 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 144 gcaaggggcagatattccaggtactggttcgcctgtctgtggcctcgctgatcacatatt 203 *F Query: 122 attcggtgaaatggatgatgaaccagatggatccgaccagtaaaaacaagaaaaaggcca 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 204 attcggtgaaatggatgatgaaccagatggatccgaccagtaaaaacaagaaaaaggcca 263 *F Query: 182 aagttctggctgaggagcagcttaagaggctagccgagcaggagggcttcaagctaagag 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 264 aagttctggctgaggagcagcttaagaggctagccgagcaggagggcttcaagctaagag 323 *F Query: 242 ggcaagagtttagcgactacgagcttatgattgcgtcacatcttgttgttcccgccgaca 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 324 ggcaagagtttagcgactacgagcttatgattgcgtcacatcttgttgttcccgccgaca 383 *F Query: 302 taacagtcagttgggccgatatcgccggtctgggattcagtcattcatgagctccgggaa 361 *F ||||||||||||||||||||||||||||||| ||||||||||||||| |||||||||||| *F Sbjct: 384 taacagtcagttgggccgatatcgccggtct-ggattcagtcattcaggagctccgggaa 442 *F Query: 362 tcggtggtgctgccaatccagcacaaggatctcttcaagcattcgaagctgtggcaggcg 421 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 443 tcggtggtgctgccaatccagcacaaggatctcttcaagcattcgaagctgtggcaggcg 502 *F Query: 422 cccaaaggcgtcctgctccatggacnancgggctgtggaaagacgctgatancgaaggcg 481 *F ||||||||||||||||||||||||| | ||||||||||||||||||||||| |||||||| *F Sbjct: 503 cccaaaggcgtcctgctccatggaccaccgggctgtggaaagacgctgatagcgaaggcg 562 *F Query: 482 acggnaaaagaagcgggaatgcgcctttatcaacttgggangtcgccattcttancgaca 541 *F |||| |||||| ||||||||||| |||||||||||| ||| ||||||||||||| ||||| *F Sbjct: 563 acggcaaaagaggcgggaatgcg-ctttatcaactt-ggacgtcgccattcttaccgaca 620 *F Query: 542 agtggtacggggaatnccangaaattgacttctgctgtcttctcgctt-catcncgaatt 600 *F ||||||||||||||| ||| |||||||||||||||||||||||||||| |||| ||||| *F Sbjct: 621 agtggtacggggaatccca-gaaattgacttctgctgtcttctcgcttgcatcgcgaatc 679 *F Query: 601 tag-catgcantaatttttattgacnaaaataactcatttttgngatttcganacat 656 *F || |||||| | |||||||||||| ||| ||||||||||| ||| |||| |||| *F Sbjct: 680 gagccatgcatt-atttttattgacgaaatagactcatttttgcgatctcgaaacat 735 *F \# *F anon-EST:Liang-1.80 == CG14996 *F \# *F anon-EST:Liang-1.83 = CG7055 *F >anon-EST:Liang-1.83 *F acgaccctat taccagcccc agtacggcgg acatcccacg ccacagccct actacgcacc *F gttctcgccg tatcagcagt cctatggccc gccacctggc tcgcactaca tgtcaccgcg *F tccgccgccg ccgcagcaca atggcaatcc cgggcatccg tatgcgccgg agcatggaag *F caatccaccg ccaccacagc agcagcaaca acaacaaccg ccaccaggac acctgcacga *F gccgagcggt ggaggacccg gggccccagg cggtggagct ggagcggcag cagcagcggc *F gcccggagcc ggagtgtatc ccacgcccgg ggcaggagcg ggaccaggag caccacctgg *F accagctgga ggagcaccgc tgggcgaggc cgcagtagct gggggagtag ctccgccagc *F ggcaacagca cctggcaagc atccagaggc ggagaagcac gaggcggact aagacaagga *F atcccaccac ccagatctag ttgtggtccc taacttatat atgaatcgat tcctggtgtt *F gtaacatagc tgtcccctcc cggaacttcc ggaaggcaaa caaccggaat gctcttaact *F tgaaatccct atttaggcgc cacacctaaa gaactccaca tcaatccatt ctcaaattaa *F agtttatatc actttttttt cgcnaaagcc taaattttaa tattttataa ctgagtgtgc *F gtccaatcct ggactgttag ctaaat *F Database: dmel_all_transcript_r320 *F >CG7055-RA type=mRNA; loc=X:8888309..8890962; ID=dalao-RA; Genome Map *F name=dalao-RA; db_xref='CG7055,FlyBase:FBgn0030093'; *F len=2654 *F Length = 2654 *F Score = 1263 bits (657), Expect = 0.0 *F Identities = 727/749 (97%), Gaps = 7/749 (0%) *F Strand = Plus / Plus *F Query: 1 acgaccctattaccagccccagtacggcggacatcccacgccacagccctactacgcacc 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1877 acgaccctattaccagccccagtacggcggacatcccacgccacagccctactacgcacc 1936 *F Query: 61 gttctcgccgtatcagcagtcctatggcccgccacctggctcgcactacatgtcaccgcg 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1937 gttctcgccgtatcagcagtcctatggcccgccacctggctcgcactacatgtcaccgcg 1996 *F Query: 121 tccgccgccgccgcagcacaatggcaatcccgggcatccgtatgcgccggagcatggaag 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1997 tccgccgccgccgcagcacaatggcaatcccgggcatccgtatgcgccggagcatggaag 2056 *F Query: 181 caatccaccgccaccacagcagcagcaacaacaacaaccgccaccaggacacctgcacga 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2057 caatccaccgccaccacagcagcagcaacaacaacaaccgccaccaggacacctgcacga 2116 *F Query: 241 gccgagcggtggaggacccggggccccaggcggtggagctggagcggcagcagcagcggc 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2117 gccgagcggtggaggacccggggccccaggcggtggagctggagcggcagcagcagcggc 2176 *F Query: 301 gcccggagccggagtgtatcccacgcccggggcaggagcgggaccaggagcaccacctgg 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2177 gcccggagccggagtgtatcccacgcccggggcaggagcgggaccaggagcaccacctgg 2236 *F Query: 361 accagctggaggagcaccgctgggcgaggccgcagtagctgggggagtagctccgccagc 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2237 accagctggaggagcaccgctgggcgaggccgcagtagctgggggagtagctccgccagc 2296 *F Query: 421 ggcaacagcacctggcaagcatccagaggcggagaagcacgaggcggactaagacaagga 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2297 ggcaacagcacctggcaagcatccagaggcggagaagcacgaggcggactaagacaagga 2356 *F Query: 481 atcccaccacccagat-ctagttgtggtccctaacttatatatgaatcgattcctggtgt 539 *F |||||||||||||||| |||||||||||||||||||||||||||||||||| |||||||| *F Sbjct: 2357 atcccaccacccagatcctagttgtggtccctaacttatatatgaatcgatccctggtgt 2416 *F Query: 540 tgtaacatagctgtcccctcccggaacttccggaaggcaaacaaccggaatgctcttaac 599 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2417 tgtaacatagctgtcccctcccggaacttccggaaggcaaacaaccggaatgctcttaac 2476 *F Query: 600 ttgaaatccctatttaggcgccacacctaaagaactccacatcaat-ccattctcaaatt 658 *F |||||||||||||||||||||||||||| ||||||||||||||||| ||||||||||||| *F Sbjct: 2477 ttgaaatccctatttaggcgccacacct-aagaactccacatcaatcccattctcaaatt 2535 *F Query: 659 aaagtttatatcacnnnnnnnncgcnaaagcctaaattttaatatttt-ataactgagtg 717 *F ||||||||||||| ||| |||||| |||||||||||||| ||||||||||| *F Sbjct: 2536 \-aagtttatatcacttttttttcgcg-aagcct-aattttaatattttaataactgagtg 2592 *F Query: 718 tgcgtccaatcctggactgttagctaaat 746 *F |||||| || ||||||||| ||||||| *F Sbjct: 2593 tgcgtcgcattctggactgtatgctaaat 2621 *F \# *F anon-EST:Liang-2.1 no sequence data available *F \# *F anon-EST:Liang-2.12 = CG17841 *F >anon-EST:Liang-2.12 *F ggcacgaatc catgagccct cgcaccgccg agtcttccag ctcctcccga tcgcccagtc *F gtccaatcgc catgtcccag acgcagtgcc gcctgtaaac aaccgccgag tgaagtccgc *F agcccgcagt catcggtagc agcagcgtca ggagtccgga atccgtttct cggtttccgc *F aacaggttgc gtcgccttcg ccaacagaag gttttagtgc gagctttgat cagagcgtgg *F agtgcgtata cgcgccagga tgcagcagcg ccgacagccg gcgaatggca acggcagagg *F aggaggaacc accgccgccg tccgtacgga ggagcagcaa caacagcagc agccattgag *F tcaggatgct ggcgccaagg aggcgggcag cacgccgggc aagacaacgg gacgcggctg *F ctatttctcg ctggccttct ccatcggttc gatgggcctg gcctgcggca gcctgtggca *F gataccgaac agcagtgacc gcatctcgct gcagcgcggc ctggtgctca cctcgctggg *F attcatctat tttgtctcgc taacggactt ctgcaacaaa tacctgctgg gagacgacgc *F atggacagcg ctttcgtcgc aaagtaccgc ctgatgatgt tccgacgtcc tggagataan *F caaacaaaat cgtctcggga atccaaggcc tcaatctcct tcttcgtggg aactcatcgt *F ctgcaaagtc gacatgcacc aaatccnttg ttg *F Database: dmel_all_transcript_r320 *F >CG17841-RA type=mRNA; *F loc=X:join(10107959..10108667,10121567..10122017, *F 10122089..10122418,10122490..10123139); ID=CG17841-RA; *F name=CG17841-RA; db_xref='CG17841,FlyBase:FBgn0028480'; *F len=2140 *F Length = 2140 *F Genome Map *F Score = 1304 bits (678), Expect = 0.0 *F Identities = 737/750 (98%), Gaps = 7/750 (0%) *F Strand = Plus / Plus *F Query: 2 gcacgaatccatgagccctcgcaccgccgagtcttccagctcctcccgatcgcccagtcg 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 48 gcacgaatccatgagccctcgcaccgccgagtcttccagctcctcccgatcgcccagtcg 107 *F Query: 62 tccaatcgccatgtcccagacgcagtgccgcctgtaaacaaccgccgagtgaagtccgca 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 108 tccaatcgccatgtcccagacgcagtgccgcctgtaaacaaccgccgagtgaagtccgca 167 *F Query: 122 gcccgcagtcatcggtagcagcagcgtcaggagtccggaatccgtttctcggtttccgca 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 168 gcccgcagtcatcggtagcagcagcgtcaggagtccggaatccgtttctcggtttccgca 227 *F Query: 182 acaggttgcgtcgccttcgccaacagaaggttttagtgcgagctttgatcagagcgtgga 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 228 acaggttgcgtcgccttcgccaacagaaggttttagtgcgagctttgatcagagcgtgga 287 *F Query: 242 gtgcgtatacgcgccaggatgcagcagcgccgacagccggcgaatggcaacggcagagga 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 288 gtgcgtatacgcgccaggatgcagcagcgccgacagccggcgaatggcaacggcagagga 347 *F Query: 302 ggaggaaccaccgccgccgtccgtacggaggagcagcaacaacagcagcagccattgagt 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 348 ggaggaaccaccgccgccgtccgtacggaggagcagcaacaacagcagcagccattgagt 407 *F Query: 362 caggatgctggcgccaaggaggcgggcagcacgccgggcaagacaacgggacgcggctgc 421 *F |||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||| *F Sbjct: 408 caggatgctggcgccaaggaggcgggcagcacgccgggcaaggcaacgggacgcggctgc 467 *F Query: 422 tatttctcgctggccttctccatcggttcgatgggcctggcctgcggcagcctgtggcag 481 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 468 tatttctcgctggccttctccatcggttcgatgggcctggcctgcggcagcctgtggcag 527 *F Query: 482 ataccgaacagcagtgaccgcatctcgctgcagcgcggcctggtgctcacctcgctggga 541 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 528 ataccgaacagcagtgaccgcatctcgctgcagcgcggcctggtgctcacctcgctggga 587 *F Query: 542 ttcatctattttgtctcgctaacggacttctgcaacaaatacctgctgggagacgacgca 601 *F ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||| *F Sbjct: 588 ttcatctattttgtctcgctaacggacttctgcaacaaatacctgct-ggagacgacgca 646 *F Query: 602 tggacagcgctttcgtcgcaaagtaccgcctgatgatgttccgacgtcctggagataanc 661 *F ||||||||||||||||||| |||||||||||||||||| ||||||||||||||||||| | *F Sbjct: 647 tggacagcgctttcgtcgc-aagtaccgcctgatgatg-tccgacgtcctggagataacc 704 *F Query: 662 aaacaaaatcgtctcgggaatccaaggcctcaatctccttcttcgtgggaactcatcgtc 721 *F |||||||||||||||| ||||| |||||||| |||||||||||||||| |||||||||| *F Sbjct: 705 \-aacaaaatcgtctcggcaatcc-aggcctcattctccttcttcgtggg-actcatcgtc 761 *F Query: 722 tgcaaagtcgacatgcaccaaatccnttgt 751 *F ||| |||||||| |||||||||||| |||| *F Sbjct: 762 tgc-aagtcgacgtgcaccaaatcctttgt 790 *F \# *F anon-EST:Liang-2.13 = ? on hth intron ? *F >anon-EST:Liang-2.13 *F gtcattatga acacacacaa aacacccaca cacacattgt aaattagtca gaatcagtaa *F atgaaaacaa aaaaaaaaaa aaacatacaa aaagcgaaga aagcccaaaa caaaaaaaaa *F atacccacac aaaacacaaa aatatattaa taaattcaga aatatacaga atcaagcgaa *F aaggagagca gaggaatttc ctagccggag cagcagcagc aacatcaacg gcagctacat *F gcaacatgcg gcagcaacat cgccggccag cagcagcagc cgaggacagt aaccgacatg *F agtgactaac cagcgacaaa cgagcttcca agaggcttcg gccacaatcc gaaccgaaaa *F acacaacgaa ccgactccac gaacgaccaa accgaactgc gaaccacgaa tcacgagtgt *F atcttgcgga tgcggatacc cattaatgtt gctatcgctg ccacggccag aatttgtttg *F ctctcctctg tgtacagtaa atgccataaa tataagttcc gaaaaagcac tgaggacagc *F cgtggctagg gccgaaagtg agtacacttt tgcctgcccc angcgccttc ccagttcatc *F tcaancgctc tcaaaaaaca ngaaaacacg taaaccacaa caaaacatca agtttntata *F tctcaaggca gantggtaaa tggntnaagg ttanacaatt acgaaancaa aanncaactg *F gggcaacaat taatt *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003687|gb|AE003687|arm:3R 6210549..6470716 *F estimated-cyto:86B2-86C3 gadfly-seqname:AE003687 *F seq_release:3 *F Length = 260168 *F Score = 929 bits (483), Expect = 0.0 Genome Map *F Identities = 534/546 (97%), Gaps = 6/546 (1%) *F Strand = Plus / Minus *F Query: 122 tacccacacaaaacacaaaaatatattaataaattcagaaatatacagaatcaagcgaaa 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 200459 tacccacacaaaacacaaaaatatattaataaattcagaaatatacagaatcaagcgaaa *F 200400 *F Query: 182 aggagagcagaggaatttcctagccggagcagcagcagcaacatcaacggcagctacatg 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 200399 aggagagcagaggaatttcctagccggagcagcagcagcaacatcaacggcagctacatg *F 200340 *F Query: 242 caacatgcggcagcaacatcgccggccagcagcagcagccgaggacagtaaccgacatga 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 200339 caacatgcggcagcaacatcgccggccagcagcagcagccgaggacagtaaccgacatga *F 200280 *F Query: 302 gtgactaaccagcgacaaacgagcttccaagaggcttcggccacaatccgaaccgaaaaa 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 200279 gtgactaaccagcgacaaacgagcttccaagaggcttcggccacaatccgaaccgaaaaa *F 200220 *F Query: 362 cacaacgaaccgactccacgaacgaccaaaccgaactgcgaaccacgaatcacgagtgta 421 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 200219 cacaacgaaccgactccacgaacgaccaaaccgaactgcgaaccacgaatcacgagtgta *F 200160 *F Query: 422 tcttgcggatgcggatacccattaatgttgctatcgctgccacggccagaatttgtttgc 481 *F |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 200159 tcttgcggatgcggatacccattaatgttgctatagctgccacggccagaatttgtttgc *F 200100 *F Query: 482 tctcctctgtgtacagtaaatgccataaatataagttccgaaaaagcactgaggacagcc 541 *F |||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 200099 tctcctctgtgtacagtaaatgccataaatataagttccg-aaaagcactgaggacagcc *F 200041 *F Query: 542 gtggctagggccgaaagtgagtacacttttgcctgccccangcgccttcccagttcatct 601 *F ||||||||||||| |||||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 200040 gtggctagggccg-aagtgagtacacttttgcctgccccacgcgccttcccagttcatct *F 199982 *F Query: 602 caancgctctcaaaaaacangaaaacacgtaaaccacaacaaaacatcaagtttntatat 661 *F || | ||||||||||||| ||||||||||||| |||||| ||||||| ||||| ||||| *F Sbjct: 199981 cacgccctctcaaaaaaca-gaaaacacgtaaa-cacaac-aaacatc-agtttatatat *F 199926 *F Query: 662 ctcaag 667 *F |||||| *F Sbjct: 199925 ctcaag 199920 *F \# *F anon-EST:Liang-2.14 = sd? *F >anon-EST:Liang-2.14 *F aaaatnatan ggatatcgat gntttgatag tatttnaagt ggaggaggtt atttatanan *F anttataagg gaatactang ttattaatt *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003500|gb|AE003500|arm:X 15436758..15740102 *F estimated-cyto:13E8-14A6 gadfly-seqname:AE003500 *F seq_release:3 *F Length = 303345 *F Score = 79.5 bits (41), Expect = 1e-14 Genome Map *F Identities = 60/73 (82%) *F Strand = Plus / Plus *F Query: 4 atnatanggatatcgatgntttgatagtatttnaagtggaggaggttatttatananant 63 *F || ||| ||||||||||| |||| || | || ||||||||||||||||||||| | | | *F Sbjct: 119088 atcatacggatatcgatgccttgacagcacttcaagtggaggaggttatttatacacagt *F 119147 *F Query: 64 tataagggaatac 76 *F | |||||||||| *F Sbjct: 119148 cacaagggaatac 119160 *F Database: dmel_all_transcript_r320 *F >CG8544-RC type=mRNA; *F loc=X:join(15549171..15549532,15550610..15550737, *F 15551010..15551148,15551437..15551567,15554201..15554241, *F 15554322..15554496,15554633..15554695,15554766..15554938, *F 15555056..15555290,15555349..15556750); ID=sd-RC; *F name=sd-RC; db_xref='CG8544,FlyBase:FBgn0003345'; *F len=2849 *F Length = 2849 *F Genome Map *F Score = 79.5 bits (41), Expect = 3e-15 *F Identities = 60/73 (82%) *F Strand = Plus / Plus *F Query: 4 atnatanggatatcgatgntttgatagtatttnaagtggaggaggttatttatananant 63 *F || ||| ||||||||||| |||| || | || ||||||||||||||||||||| | | | *F Sbjct: 1944 atcatacggatatcgatgccttgacagcacttcaagtggaggaggttatttatacacagt 2003 *F Query: 64 tataagggaatac 76 *F | |||||||||| *F Sbjct: 2004 cacaagggaatac 2016 *F \# *F anon-EST:Liang-2.15 == CG15100 *F \# *F anon-EST:Liang-2.16 = CG4602? match bad, but note n's in query seq *F >anon-EST:Liang-2.16 *F tcatacnggt tctggccata cccgaggant atcgggccct gnanatgntt aataacggaa *F cnantgtgcc gggantccan annccggact ccaagctacn gnccgaagtc attaancgca *F tcgagggana gntgccgcat caagtgatca anacntanta ccccaagttg gtggaattca *F atctgccgga gtanccggcc ttaccctcnt tctacgatgc gcgcaaaatc taggagattc *F ggngcaccat tatcgtgtgn natnttaaga acnagtggcg cctaaangat ctgatggaat *F gctttcancg nnctggggag gtgaagtatg cncgctgggc cgagaaggat ancaanacgt *F actgcattga ttgatttctg cgaacagacc ancattattc acgctctgcg catgcagggn *F caggagttca agggtggcca nctaanncgt tancaatcna cg *F Database: dmel_all_transcript_r320 *F >CG4602-RA type=mRNA; loc=2L:9905316..9907453; ID=Srp54-RA; Genome Map *F name=Srp54-RA; db_xref='CG4602,FlyBase:FBgn0024285'; *F len=2138 *F Length = 2138 *F Score = 639 bits (332), Expect = 0.0 *F Identities = 397/445 (89%), Gaps = 1/445 (0%) *F Strand = Plus / Plus *F Query: 1 tcatacnggttctggccatacccgaggantatcgggccctgnanatgnttaataacggaa 60 *F |||||| |||||||||||||||||||| |||||||||||| | ||| | || ||||||| *F Sbjct: 389 tcatacccgttctggccatacccgaggagtatcgggccctggagatgctcaagaacggaa 448 *F Query: 61 cnantgtgccgggantccanannccggactccaagctacngnccgaagtcattaancgca 120 *F | | |||||||||| |||| | |||||||||||||||| | ||||||||||||| |||| *F Sbjct: 449 ccattgtgccgggactccagaagccggactccaagctaccgcccgaagtcattaaccgca 508 *F Query: 121 tcgaggganagntgccgcatcaagtgatcaanacntantaccccaagttggtggaattca 180 *F |||||||| || ||||||| ||||||||||| || || ||||||||||||||||||||| *F Sbjct: 509 tcgagggacagctgccgcagcaagtgatcaagacgtacgaccccaagttggtggaattca 568 *F Query: 181 atctgccggagtanccggccttaccctcnttctacgatgcgcgcaaaatctaggagattc 240 *F ||||||||||||| |||||||||||||| ||||||||||||||||||||| ||||||||| *F Sbjct: 569 atctgccggagtacccggccttaccctcgttctacgatgcgcgcaaaatcgaggagattc 628 *F Query: 241 ggngcaccattatcgtgtgnnatnttaagaacnagtggcgcctaaangatctgatggaat 300 *F || |||||||||||||||| || |||||||| ||||||| ||| | ||||||||||||| *F Sbjct: 629 ggcgcaccattatcgtgtgcgatgttaagaacgagtggcggctagacgatctgatggaat 688 *F Query: 301 gctttcancgnnctggggaggtgaagtatgcncgctgggccgagaaggatancaanacgt 360 *F ||||||| || ||||||||||||||||||| || |||||||||||||||| ||| |||| *F Sbjct: 689 gctttcagcgcgctggggaggtgaagtatgcccgttgggccgagaaggataacaagacgt 748 *F Query: 361 actgcattgattgatttctgcgaacagaccancattattcacgctctgcgcatgcagggn 420 *F |||||| ||||||| |||||||||||||||| |||||||||||| |||||||||||||| *F Sbjct: 749 actgca-tgattgagttctgcgaacagaccagcattattcacgccctgcgcatgcagggc 807 *F Query: 421 caggagttcaagggtggccanctaa 445 *F |||||||||||||||||||| |||| *F Sbjct: 808 caggagttcaagggtggccatctaa 832 *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003626|gb|AE003626|arm:2L 9701091..9966189 *F estimated-cyto:30C5-30F5 gadfly-seqname:AE003626 *F seq_release:3 *F Length = 265099 *F Score = 639 bits (332), Expect = 0.0 Genome Map *F Identities = 397/445 (89%), Gaps = 1/445 (0%) *F Strand = Plus / Plus *F Query: 1 tcatacnggttctggccatacccgaggantatcgggccctgnanatgnttaataacggaa 60 *F |||||| |||||||||||||||||||| |||||||||||| | ||| | || ||||||| *F Sbjct: 204614 tcatacccgttctggccatacccgaggagtatcgggccctggagatgctcaagaacggaa *F 204673 *F Query: 61 cnantgtgccgggantccanannccggactccaagctacngnccgaagtcattaancgca 120 *F | | |||||||||| |||| | |||||||||||||||| | ||||||||||||| |||| *F Sbjct: 204674 ccattgtgccgggactccagaagccggactccaagctaccgcccgaagtcattaaccgca *F 204733 *F Query: 121 tcgaggganagntgccgcatcaagtgatcaanacntantaccccaagttggtggaattca 180 *F |||||||| || ||||||| ||||||||||| || || ||||||||||||||||||||| *F Sbjct: 204734 tcgagggacagctgccgcagcaagtgatcaagacgtacgaccccaagttggtggaattca *F 204793 *F Query: 181 atctgccggagtanccggccttaccctcnttctacgatgcgcgcaaaatctaggagattc 240 *F ||||||||||||| |||||||||||||| ||||||||||||||||||||| ||||||||| *F Sbjct: 204794 atctgccggagtacccggccttaccctcgttctacgatgcgcgcaaaatcgaggagattc *F 204853 *F Query: 241 ggngcaccattatcgtgtgnnatnttaagaacnagtggcgcctaaangatctgatggaat 300 *F || |||||||||||||||| || |||||||| ||||||| ||| | ||||||||||||| *F Sbjct: 204854 ggcgcaccattatcgtgtgcgatgttaagaacgagtggcggctagacgatctgatggaat *F 204913 *F Query: 301 gctttcancgnnctggggaggtgaagtatgcncgctgggccgagaaggatancaanacgt 360 *F ||||||| || ||||||||||||||||||| || |||||||||||||||| ||| |||| *F Sbjct: 204914 gctttcagcgcgctggggaggtgaagtatgcccgttgggccgagaaggataacaagacgt *F 204973 *F Query: 361 actgcattgattgatttctgcgaacagaccancattattcacgctctgcgcatgcagggn 420 *F |||||| ||||||| |||||||||||||||| |||||||||||| |||||||||||||| *F Sbjct: 204974 actgca-tgattgagttctgcgaacagaccagcattattcacgccctgcgcatgcagggc *F 205032 *F Query: 421 caggagttcaagggtggccanctaa 445 *F |||||||||||||||||||| |||| *F Sbjct: 205033 caggagttcaagggtggccatctaa 205057 *F \# *F >anon-EST:Liang-2.19 = CG32177 *F >anon-EST:Liang-2.19 *F gatgatcagt tgggaccgcc aaaggcggac ttcagtgctc cgccaccgca cgaggcgaac *F gttggccatg gccagcaggt agcactgccg gctcagatgc caccgctggc cttggccaca *F cccgatccca gtcagatccc gatgcagatg caagtgcagc tgccgggcca ggcggacctt *F atgaatgcac aactgccgcc agaaatacac gggaagttgc ccacgtacga ggaggttcaa *F atggaaaagt cgctgaacgg agagctgccg cccgcctttt tgactttacc ctcctcgcag *F cagctaccgc cgccgccgaa tccgctactg ccgccaaatc cgctgcgcga tggatccgct *F gctgtgcgat ctgcccagcc ggcactcacg ttcatcgcca tcgatgccag cgatccggaa *F aatagtctgt ccaccaccga caacttgctc ggcacggaca tcatgttcat cacggccttc *F attgtggccc ttttgtttaa ctggatcggc ttcctgatgc tcacctgttt ctgtcacaca *F atcgccgccc gatatggagc attgtcggga ttcggattgt cnctgggtaa atggacgctg *F atngtgaagc actcgacggg ncttggcctc gcacgaaaaa ctcctggctc tggtggctga *F tctgtgcctt tggcttcctg ataagcatac gcgcnttann cagtatgtca gcattaaagc *F nnatcgttgg *F Database: dmel_all_transcript_r320 *F >CG32177-RA type=mRNA; *F loc=3L:complement(17596318..17598125,17602871..17603155); Genome *F Map *F ID=CG32177-RA; name=CG32177-RA; *F db_xref='CG32177,FlyBase:FBgn0052177'; len=2093 *F Length = 2093 *F Score = 1265 bits (658), Expect = 0.0 *F Identities = 699/715 (97%), Gaps = 3/715 (0%) *F Strand = Plus / Plus *F Query: 1 gatgatcagttgggaccgccaaaggcggacttcagtgctccgccaccgcacgaggcgaac 60 *F |||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| *F Sbjct: 318 gatgatcagttgggaccgccaaaggcggacttcagtgctccgccaccgtacgaggcgaac 377 *F Query: 61 gttggccatggccagcaggtagcactgccggctcagatgccaccgctggccttggccaca 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 378 gttggccatggccagcaggtagcactgccggctcagatgccaccgctggccttggccaca 437 *F Query: 121 cccgatcccagtcagatcccgatgcagatgcaagtgcagctgccgggccaggcggacctt 180 *F ||||||||||||||||||||||| ||||||||||||||||||||| |||||||||||||| *F Sbjct: 438 cccgatcccagtcagatcccgatacagatgcaagtgcagctgccgagccaggcggacctt 497 *F Query: 181 atgaatgcacaactgccgccagaaatacacgggaagttgcccacgtacgaggaggttcaa 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 498 atgaatgcacaactgccgccagaaatacacgggaagttgcccacgtacgaggaggttcaa 557 *F Query: 241 atggaaaagtcgctgaacggagagctgccgcccgcctttttgactttaccctcctcgcag 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 558 atggaaaagtcgctgaacggagagctgccgcccgcctttttgactttaccctcctcgcag 617 *F Query: 301 cagctaccgccgccgccgaatccgctactgccgccaaatccgctgcgcgatggatccgct 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 618 cagctaccgccgccgccgaatccgctactgccgccaaatccgctgcgcgatggagccgct 677 *F Query: 361 gctgtgcgatctgcccagccggcactcacgttcatcgccatcgatgccagcgatccggaa 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 678 gctgtgcgatctgcccagccggcactcacgttcatcgccatcgatgccagcgatccggaa 737 *F Query: 421 aatagtctgtccaccaccgacaacttgctcggcacggacatcatgttcatcacggccttc 480 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 738 aatagtctgtccaccaccgacaacttgctcggcacggacatcatgttcatcacggccttc 797 *F Query: 481 attgtggcccttttgtttaactggatcggcttcctgatgctcacctgtttctgtcacaca 540 *F ||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 798 attgtggcctttttgtttaactggatcggcttcctgatgctcacctgtttctgtcacaca 857 *F Query: 541 atcgccgcccgatatggagcattgtcgggattcggattgtcnctgggtaaatggacgctg 600 *F ||||||||||||||||||||||||||||||||||||||||| |||| ||||||||||||| *F Sbjct: 858 atcgccgcccgatatggagcattgtcgggattcggattgtcgctggctaaatggacgctg 917 *F Query: 601 atngtgaagcactcgacgggncttggcctcgcacgaaaaactcctggctctggtggctga 660 *F || |||||||||||||| || |||||||||||||| |||||||||||||||||||||||| *F Sbjct: 918 atcgtgaagcactcgac-ggacttggcctcgcacg-aaaactcctggctctggtggctga 975 *F Query: 661 tctgtgcctttggcttcctgataagcatacgcgcntt-anncagtatgtcagcat 714 *F |||||||||||||||||||||| ||||||||||| || | |||||||||||||| *F Sbjct: 976 tctgtgcctttggcttcctgatcagcatacgcgctttaatacagtatgtcagcat 1030 *F \# *F anon-EST:Liang-2.2 = CG15154 *F >anon-EST:Liang-2.2 *F gagtcagcaa tatgttgtcg gcgggaacgc aacacagcag caagccagtt tccagaagca *F ataatagcaa cagttgctgc cgcagtcgca gcagtaaagc actttcaatg ggagcagcaa *F catcctccgc ctccggatcc gcatccacat ccgtgtccac atccacatcc tcgaagcgac *F gatgctgcaa gtgcaagtgc cgcgacgcca tccgaggatt aagggatatg ctgccgagca *F gcagcagttc cgtgagcaat aaaaaggatg ccgctgcggt ggtggcgatg gttccgagga *F aatcccggac agctgaccgc cgctccacac cgcccacgct gggcacatcg agtggtggct *F cacagcgccg gatgcagagc caaaggatac gaaattccgt cgccgttccg gacgccagtc *F atcaccatca tcaagctcat cattcggatc acatcgccgg gcttcgtggt cgagtcgccc *F aatggcggcc gcgtaactgt ggtcaccgag cccgcccccc agtcgcccag actccgcccc *F ggccacgtcc cctggccacg gtcgcccccg tcggaggggc tggccatgcc ggaatcggag *F ccgcccgcaa catgaccgnt gcactcgcaa aatcgacttc atgcactgcc tggttnccga *F tttcgagaag attaacgaan agcaagcttc taacgggggg caanatggcc cgcttaccaa *F ggnggggcaa cntggt *F Database: dmel_all_transcript_r320 *F >CG15154-RB type=mRNA; *F loc=2L:complement(18116751..18117927,18117979..18118237, *F 18118526..18119333,18119827..18120663, *F 18130123..18130493); ID=Socs36E-RB; name=Socs36E-RB; *F db_xref='CG15154,FlyBase:FBgn0041184'; len=3452 *F Length = 3452 *F Genome Map *F Score = 1210 bits (629), Expect = 0.0 *F Identities = 664/672 (98%), Gaps = 4/672 (0%) *F Strand = Plus / Plus *F Query: 2 agtcagcaatatgttgtcggcgggaacgcaacacagcagcaagccagtttccagaagcaa 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1162 agtcagcaatatgttgtcggcgggaacgcaacacagcagcaagccagtttccagaagcaa 1221 *F Query: 62 taatagcaacagttgctgccgcagtcgcagcagtaaagcactttcaatgggagcagcaac 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1222 taatagcaacagttgctgccgcagtcgcagcagtaaagcactttcaatgggagcagcaac 1281 *F Query: 122 atcctccgcctccggatccgcatccacatccgtgtccacatccacatcctcgaagcgacg 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1282 atcctccgcctccggatccgcatccacatccgtgtccacatccacatcctcgaagcgacg 1341 *F Query: 182 atgctgcaagtgcaagtgccgcgacgccatccgaggattaagggatatgctgccgagcag 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1342 atgctgcaagtgcaagtgccgcgacgccatccgaggattaagggatatgctgccgagcag 1401 *F Query: 242 cagcagttccgtgagcaataaaaaggatgccgctgcggtggtggcgatggttccgaggaa 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1402 cagcagttccgtgagcaataaaaaggatgccgctgcggtggtggcgatggttccgaggaa 1461 *F Query: 302 atcccggacagctgaccgccgctccacaccgcccacgctgggcacatcgagtggtggctc 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1462 atcccggacagctgaccgccgctccacaccgcccacgctgggcacatcgagtggtggctc 1521 *F Query: 362 acagcgccggatgcagagccaaaggatacgaaattccgtcgccgttccggacgccagtca 421 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| || *F Sbjct: 1522 acagcgccggatgcagagccaaaggatacgaaattccgtcgccgttccggacgccagcca 1581 *F Query: 422 tcaccatcatcaagctcatcattcggatcacatcgccgggcttcgtggtcgagtcgccca 481 *F ||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1582 tcaccatcatc-agctcatcattcggatcacatcgccgggcttcgtggtcgagtcgccca 1640 *F Query: 482 atggcggccgcgtaactgtggtcaccgagcccgccccccagtcg-cccagactccgcccc 540 *F |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 1641 atggcggccgcgtaactgtggtcaccgagcccgccccccagtcgccccagactccgcccc 1700 *F Query: 541 ggccacgtcccctggccacggtcgcccccgtcggaggggctggccatgccggaatcggag 600 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 1701 ggccacgtcccctggccacggtcgcccccgccggaggggctggccatgccggaatcggag 1760 *F Query: 601 ccgcccgcaacatgaccgntgcactcgcaaaatcgacttcatgcactgcctggttnccga 660 *F |||||||||||||||||| ||||||||| |||||||||||||||||||||||||| |||| *F Sbjct: 1761 ccgcccgcaacatgaccg-tgcactcgc-aaatcgacttcatgcactgcctggttcccga 1818 *F Query: 661 tttcgagaagat 672 *F | |||||||||| *F Sbjct: 1819 tctcgagaagat 1830 *F \# *F anon-EST:Liang-2.23 = roo element *F >anon-EST:Liang-2.23 *F tgnaattaat ccaaaaacta aaaggatcaa ttgacaatat aatggagatc tatgcttgga *F gtgattccac gattacctta gcatggatta acagtggtca aagtaagatc aaatttataa *F aaagaagaac ggatgacatt cggaaattaa aaaatactga atggaatcat gttaagtcag *F aggataatcc agcagattta gcatccaggg gagtggattc taaccagttg atcaactgtg *F atttttggtg gaaaggtccg aaatggctag cagacccaaa agaactttgg cctcggcagc *F agtctgtaga agaacctgtc ttaataaata cggtattaaa tgacaaaata gatgatccta *F tttacgaatt aatagaaagg tattccagta tagaaaaact tatacgtata atagcataca *F taaatagatt cgtgcagatg aaaacaagaa ataaagccta ttcatcaatt atttcagtaa *F aggagataag aatagcggaa acagttgtta ttaagaaaca acaagaatac cagtttaggc *F aagagataaa gtgccttaaa atcaaaaagg aaatcaagac aaatataaaa tattgtcatt *F gaatccattt ttggacaagg gatggggttc taaganttgg aggaagattg caaaattcca *F atgcagaatt taatggttaa acatccaatn cattttagaa aaaatgccan ctaacaagct *F tantaaataa aaaatgcnca t *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003418|gb|AE003418|arm:X 288957..603388 *F estimated-cyto:1B9-1D1 gadfly-seqname:AE003418 *F seq_release:3 *F Length = 314432 *F Score = 1308 bits (680), Expect = 0.0 Genome Map *F Identities = 728/739 (98%), Gaps = 6/739 (0%) *F Strand = Plus / Plus *F Query: 4 aattaatccaaaaactaaaaggatcaattgacaatataatggagatctatgcttggagtg 63 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 300791 aattaatccaaagactaaaaggatcaattgacaatataatggagatctatgcttggagtg *F 300850 *F Query: 64 attccacgattaccttagcatggattaacagtggtcaaagtaagatcaaatttataaaaa 123 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 300851 attccacgattaccttagcatggattaacagtggtcaaagtaagatcaaatttataaaaa *F 300910 *F Query: 124 gaagaacggatgacattcggaaattaaaaaatactgaatggaatcatgttaagtcagagg 183 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 300911 gaagaacggatgacattcggaaattaaaaaatactgaatggaatcatgttaagtcagagg *F 300970 *F Query: 184 ataatccagcagatttagcatccaggggagtggattctaaccagttgatcaactgtgatt 243 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 300971 ataatccagcagatttagcatccaggggagtggattctaaccagttgatcaactgtgatt *F 301030 *F Query: 244 tttggtggaaaggtccgaaatggctagcagacccaaaagaactttggcctcggcagcagt 303 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301031 tttggtggaaaggtccgaaatggctagcagacccaaaagaactttggcctcggcagcagt *F 301090 *F Query: 304 ctgtagaagaacctgtcttaataaatacggtattaaatgacaaaatagatgatcctattt 363 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301091 ctgtagaagaacctgtcttaataaatacggtattaaatgacaaaatagatgatcctattt *F 301150 *F Query: 364 acgaattaatagaaaggtattccagtatagaaaaacttatacgtataatagcatacataa 423 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301151 acgaattaatagaaaggtattccagtatagaaaaacttatacgtataatagcatacataa *F 301210 *F Query: 424 atagattcgtgcagatgaaaacaagaaataaagcctattcatcaattatttcagtaaagg 483 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301211 atagattcgtgcagatgaaaacaagaaataaagcctattcatcaattatttcagtaaagg *F 301270 *F Query: 484 agataagaatagcggaaacagttgttattaagaaacaacaagaataccagtttaggcaag 543 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 301271 agataagaatagcggaaacagttgttattaagaaacaacaagaataccagtttaggcaag *F 301330 *F Query: 544 agataaagtgccttaaaatcaaaaaggaaatcaagacaaat-ataaaatattgtcattga 602 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 301331 agataaagtgccttaaaatcaaaaaggaaatcaagacaaataataaaatattgtcattga *F 301390 *F Query: 603 atccatttttggacaagggatggggttctaaganttggaggaagattgcaaaattccaat 662 *F |||||||||||||||| |||||||||||||||| |||||||||||||||||||||||||| *F Sbjct: 301391 atccatttttggacaa-ggatggggttctaagagttggaggaagattgcaaaattccaat *F 301449 *F Query: 663 gcagaatttaatggttaaacatccaatncattttagaaaaaatgccanctaacaagctta 722 *F |||||||||||| |||||||||||||| |||||||| |||||||||| |||||||||||| *F Sbjct: 301450 gcagaatttaat-gttaaacatccaat-cattttag-aaaaatgccacctaacaagctta *F 301506 *F Query: 723 ntaaataaaaaatgcncat 741 *F | |||||||||||| ||| *F Sbjct: 301507 tt-aataaaaaatgctcat 301524 *F Database: dmel_all_transposon_r320 *F Score E *F Sequences producing significant alignments: (bits) Value *F FBti0019658 type=transposable_element; loc=X:20509196..20516740;... 1308 0.0 *F FBti0019083 type=transposable_element; loc=X:complement(18688083... 1308 0.0 *F FBti0019068 type=transposable_element; loc=X:complement(16071742... 1308 0.0 *F FBti0019060 type=transposable_element; loc=X:14667929..14677127;... 1308 0.0 *F FBti0019051 type=transposable_element; loc=X:13835738..13844829;... 1308 0.0 *F FBti0019630 type=transposable_element; loc=X:11474279..11481367;... 1308 0.0 *F FBti0019597 type=transposable_element; loc=X:6865677..6874777; I... 1308 0.0 *F FBti0019576 type=transposable_element; loc=X:4731680..4736379; I... 1308 0.0 *F FBti0019561 type=transposable_element; loc=X:complement(3365404.... 1308 0.0 *F FBti0019556 type=transposable_element; loc=X:complement(3251584.... 1308 0.0 *F FBti0019553 type=transposable_element; loc=X:complement(2976997.... 1308 0.0 *F FBti0019547 type=transposable_element; loc=X:complement(2580615.... 1308 0.0 *F FBti0019544 type=transposable_element; loc=X:2156665..2164959; I... 1308 0.0 *F FBti0019532 type=transposable_element; loc=X:821248..829985; ID=... 1308 0.0 *F FBti0019526 type=transposable_element; loc=X:585093..592827; ID=... 1308 0.0 *F FBti0020394 type=transposable_element; loc=3R:complement(2770289... 1308 0.0 *F FBti0019450 type=transposable_element; loc=3R:24573429..24578987... 1308 0.0 *F FBti0019439 type=transposable_element; loc=3R:22822547..22831625... 1308 0.0 *F FBti0019435 type=transposable_element; loc=3R:complement(2219146... 1308 0.0 *F FBti0019432 type=transposable_element; loc=3R:21647833..21656956... 1308 0.0 *F FBti0019431 type=transposable_element; loc=3R:complement(2154097... 1308 0.0 *F FBti0019421 type=transposable_element; loc=3R:19651837..19660930... 1308 0.0 *F FBti0019416 type=transposable_element; loc=3R:complement(1805623... 1308 0.0 *F FBti0019406 type=transposable_element; loc=3R:complement(1598248... 1308 0.0 *F FBti0019374 type=transposable_element; loc=3R:10020224..10029315... 1308 0.0 *F FBti0019357 type=transposable_element; loc=3R:complement(7205031... 1308 0.0 *F FBti0020187 type=transposable_element; loc=3L:21223479..21232579... 1308 0.0 *F etc ect *F \# *F anon-EST:Liang-2.24 == CG11525 *F \# *F anon-EST:Liang-2.28 = CG2210 *F >anon-EST:Liang-2.28 *F gattcttttc tgtaatctcg gcgacaatgg cggctaacaa ggagaggact ttcatcatgg *F tcaagcccga tggcgtccag cgcgggctcg tcggcaagat catcgagcgc ttcgagcaga *F agggcttcaa gctggtcgcc ctgaagttca cctgggcctc caaggagctg ctggagaagc *F actacgctga tctgtccgcc cgccccttct tccccggact cgtgaactac atgaactccg *F gccccgtggt gcccatggtg tgggagggtc tgaatgtggt caagaccggt cgccagatgc *F tcggcgccac caaccccgcc gactcgctgc ccggcaccat ccgcggtgac ttctgcattc *F aggtcggacg caacatcatc cacggctccg atgccgtcga gtctgccgan aaggagatcg *F ccctgtggtt caacgaaaan gagctggtca cctggacccc ggccgccaag gactggatct *F acgaatagac ggctacttta actgtctgcc ctcgtctaag ctgaatacag attgattctt *F ctaaagaaat aaaattccac aataattact aanaaannnn aaanaaacan ntgctgcggc *F cgccgaaant ccggtcccct aaaagtnagt cgtattaant tcgataagcc angcttgcat *F taaatgaant cgggccaacn cccgggggaa aagcgggttt gcgtattggg gcgctcnncc *F nctttcctcc gctcaatgan ctcgctggcg cncgggtt *F Database: dmel_all_ *F >CG2210-RA type=mRNA; *F loc=3R:complement(27560260..27560678, *F 27560858..27561121); ID=awd-RA; name=awd-RA; *F db_xref='CG2210,FlyBase:FBgn0000150'; len=683 *F Length = 683 *F Genome Map *F Score = 1088 bits (566), Expect = 0.0 *F Identities = 568/570 (99%) *F Strand = Plus / Plus *F Query: 2 attcttttctgtaatctcggcgacaatggcggctaacaaggagaggactttcatcatggt 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 111 attcttttctgtaatctcggcgacaatggcggctaacaaggagaggactttcatcatggt 170 *F Query: 62 caagcccgatggcgtccagcgcgggctcgtcggcaagatcatcgagcgcttcgagcagaa 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 171 caagcccgatggcgtccagcgcgggctcgtcggcaagatcatcgagcgcttcgagcagaa 230 *F Query: 122 gggcttcaagctggtcgccctgaagttcacctgggcctccaaggagctgctggagaagca 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 231 gggcttcaagctggtcgccctgaagttcacctgggcctccaaggagctgctggagaagca 290 *F Query: 182 ctacgctgatctgtccgcccgccccttcttccccggactcgtgaactacatgaactccgg 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 291 ctacgctgatctgtccgcccgccccttcttccccggactcgtgaactacatgaactccgg 350 *F Query: 242 ccccgtggtgcccatggtgtgggagggtctgaatgtggtcaagaccggtcgccagatgct 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 351 ccccgtggtgcccatggtgtgggagggtctgaatgtggtcaagaccggtcgccagatgct 410 *F Query: 302 cggcgccaccaaccccgccgactcgctgcccggcaccatccgcggtgacttctgcattca 361 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 411 cggcgccaccaaccccgccgactcgctgcccggcaccatccgcggtgacttctgcattca 470 *F Query: 362 ggtcggacgcaacatcatccacggctccgatgccgtcgagtctgccganaaggagatcgc 421 *F |||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||| *F Sbjct: 471 ggtcggacgcaacatcatccacggctccgatgccgtcgagtctgccgagaaggagatcgc 530 *F Query: 422 cctgtggttcaacgaaaangagctggtcacctggaccccggccgccaaggactggatcta 481 *F |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 531 cctgtggttcaacgaaaaggagctggtcacctggaccccggccgccaaggactggatcta 590 *F Query: 482 cgaatagacggctactttaactgtctgccctcgtctaagctgaatacagattgattcttc 541 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 591 cgaatagacggctactttaactgtctgccctcgtctaagctgaatacagattgattcttc 650 *F Query: 542 taaagaaataaaattccacaataattacta 571 *F |||||||||||||||||||||||||||||| *F Sbjct: 651 taaagaaataaaattccacaataattacta 680 *F \# *F anon-EST:Liang-2.31 = not in genome *F >anon-EST:Liang-2.31 *F aannncntta agctgtagtg agcnagtaaa atgtnagnan ttggagnact tnagtgnaat *F aatcgtnatt cctgtnanaa gg *F Database: na_all.dros *F Only self hit *F Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, *F GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) 2,262,611 *F sequences; 10,883,439,008 total letters *F If you have any problems or questions with the results of this search *F please refer to the BLAST FAQs *F No significant similarity found. For reasons why, click here. *F \# *F anon-EST:Liang-2.39 = in how intron ? *F >anon-EST:Liang-2.39 *F acttattttg cagccaaatc taaaaatcag atcaaatcta tcgaatacac aagcgcatat *F ataaacgact cattccaaag aagcagagaa tttaaatgtt acaaagtgaa atagaataca *F gaatacgana aacaggtcga taattccccc tcagttggna atttggcttc naagctgctg *F ccttcagctt cagtttgang caagcgaatg gtagataagt aaggangtat gcaatatcta *F ttagacctaa gttgtaacct gtatctgang atgttcacat cnatttannc atatnaccga *F natgtanacc tatgnaancc antatctaca tgtntcntat cgtaatnntt tagtatngcg *F ccantaagca gcagcntcgt ttggcgacta nccgcgagca nccctatcag ncgancaacn *F gtcgggntgc caagccanng cccggcgggt ttcaattgag antacagtng atncnatgnn *F tngcttgaat tgganganaa ttcngncgca ntagtaantc agnacnttnn ccactcntan *F gancacacca ctntttggca aaaag *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003737|gb|AE003737|arm:3R 17772253..17950503 *F estimated-cyto:93F10-94A2 gadfly-seqname:AE003737 *F seq_release:3 *F Length = 178251 *F Score = 631 bits (328), Expect = e-180 Genome Map *F Identities = 397/432 (91%), Gaps = 5/432 (1%) *F Strand = Plus / Plus *F Query: 1 acttattttgcagccaaatctaaaaatcagatcaaatctatcgaatacacaagcgcatat 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 118533 acttattttgcagccaaatctaaaaatcagatcaaatctatcgaatacacaagcgcatat *F 118592 *F Query: 61 ataaacgactcattccaaagaagcagagaatttaaatgttacaaagtgaaatagaataca 120 *F ||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||| *F Sbjct: 118593 ataaacgactcattccaaagaagcagagaatttaaatgttacaaagtgaaagagaataca *F 118652 *F Query: 121 gaatacganaaacaggtcgataattccccctcagttggnaatttggcttcnaagctgctg 180 *F |||||||| ||||||||||||||||||||||||||||| ||||||||||| |||||||| *F Sbjct: 118653 gaatacgaaaaacaggtcgataattccccctcagttggcaatttggcttcc-agctgctg *F 118711 *F Query: 181 ccttcagcttcagtttgangcaagcgaatggtagataagtaaggangtatgcaatatcta 240 *F |||||||||||||||||| |||||||||||||||||||||||||| |||||||||||||| *F Sbjct: 118712 ccttcagcttcagtttga-gcaagcgaatggtagataagtaaggatgtatgcaatatcta *F 118770 *F Query: 241 ttagacctaagttgtaacctgtatctgangatgttcacatcnatttanncatatnaccga 300 *F |||||||||||||||| |||||| |||| |||||||||||| ||||| ||||| ||||| *F Sbjct: 118771 ttagacctaagttgtaccctgtagctgatgatgttcacatctatttatacatataaccga *F 118830 *F Query: 301 natgtanacctatgnaanccantatctacatgtntcntatcgtaatnntttagtatngcg 360 *F ||||| ||||||| || ||| ||||||||||| || |||||| || ||||||| ||| *F Sbjct: 118831 tatgtaaacctatgcaaaccaatatctacatgtatcgtatcgt-atattttagta-ggcg *F 118888 *F Query: 361 ccantaagcagcagcntcgtttggcgactanccgcgagcanccctatcagncgancaacn 420 *F ||| ||||||||||| |||||||||||||| ||||||||| ||||||||| ||| |||| *F Sbjct: 118889 ccattaagcagcagcgtcgtttggcgactaaccgcgagcatccctatcag-cgagcaacg *F 118947 *F Query: 421 gtcgggntgcca 432 *F ||||| ||||| *F Sbjct: 118948 gtcggagtgcca 118959 *F \# *F anon-EST:Liang-2.40 = no sequence data available *F \# *F anon-EST:Liang-2.41 = CG32662 *F >anon-EST:Liang-2.41 *F tgaaagctaa aagagaaaga gagggctgaa aagcttaaag acctagaaaa agaantaaag *F ctaaaggaaa aggaggaaca gctcaaggag aaggaaaagg agcttaaact caaggagaag *F aaggagaaag acaaggtcaa agagaaggag aagtccctgg aaagcgaaaa gctcctcatc *F tcggcaaccg ttagcaatcc atggaggcga gtcgtcnagg atactccacc caaactgcct *F gcggttcagg actatcccan ccttggcaag aagcccacta aagcgagtcc ggagaagaag *F cgggatgaaa aactgctgcc tggactgact actcccccca aagaggtgaa cgacaccttt *F gaggacttct tgagcggctt aaagccactg gaggccttgc nanctctccc tgctctcgaa *F cccctggagg ttaangagga ctcnaaaaag gaatctgtca gtctaatcaa ctttgaaagt *F ccgttgcang agacaagctt cnataccgcg gaagatttcc caccgccacg tggtttcacc *F gaaacagaat ttgnttcctc gcccttgttg cggttccctt gcactatgaa anatnaacag *F ggaacgcatc cgggggaaan gganggccga aattgcancc ctganggtgg gtaanaaaca *F ccgggaaacc ggttanacan ttaccctngg ananncnggg nnccngnann naatcaactc *F cnaaatcgaa ananctccnc cgggatttga gggaaatttt tcgtncatnt g *F Database: dmel_all_transcript_r320 *F >CG32662-RA type=mRNA; loc=X:11560075..11564160; ID=CG32662-RA; Genome Map *F name=CG32662-RA; db_xref='CG32662,FlyBase:FBgn0052662'; *F len=4086 *F Length = 4086 *F Score = 760 bits (395), Expect = 0.0 *F Identities = 468/487 (96%), Gaps = 9/487 (1%) *F Strand = Plus / Plus *F Query: 129 agacaaggtcaaagagaaggagaagtccctggaaagcgaaaagctcctcatctcggcaac 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2205 agacaaggtcaaagagaaggagaagtccctggaaagcgaaaagctcctcatctcggcaac 2264 *F Query: 189 cgttagcaatccatggaggcgagtcgtcnaggatactccacccaaactgcctgcggttca 248 *F |||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||| *F Sbjct: 2265 cgttagcaatccatggaggcgagtcgtcgaggatactccacccaaactgcctgcggttca 2324 *F Query: 249 ggactatcccanccttggcaagaagcccactaaagcgagtccggagaagaagcgggatga 308 *F ||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2325 ggactatcccagccttggcaagaagcccactaaagcgagtccggagaagaagcgggatga 2384 *F Query: 309 aaaactgctgcctggactgactactccccccaaagaggtgaacgacacctttgaggactt 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2385 aaaactgctgcctggactgactactccccccaaagaggtgaacgacacctttgaggactt 2444 *F Query: 369 cttgagcggcttaaagccactggaggccttgcnanctctccctgctctcgaacccctgga 428 *F |||||||||||||||||||||||||||||||| | ||||||||||||||||||||||||| *F Sbjct: 2445 cttgagcggcttaaagccactggaggccttgccacctctccctgctctcgaacccctgga 2504 *F Query: 429 ggttaangaggactcnaaaaaggaatctgtcagtctaatcaactttgaaagtccgttgca 488 *F |||||| |||||||| |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2505 ggttaaggaggactcgaaaaaggaatctgtcagtctaatcaactttgaaagtccgttgca 2564 *F Query: 489 ngagacaagcttcnataccgcggaagattt-cccaccgccacgtggtttcaccgaaacag 547 *F ||||| |||||| |||||||||||||||| ||||||||||||||||||||||| ||||| *F Sbjct: 2565 ggagac-agcttccataccgcggaagatttccccaccgccacgtggtttcaccg-aacag 2622 *F Query: 548 aatttgnttcctcgcccttgttgcggttcccttgcactatgaaanatnaacagggaacgc 607 *F |||||| |||||| ||||| |||||||||| |||||||||||| || |||| ||||||| *F Sbjct: 2623 aatttga-tcctcg-ccttg-tgcggttccc-tgcactatgaaa-atgaaca-ggaacgc 2676 *F Query: 608 atccggg 614 *F ||||||| *F Sbjct: 2677 atccggg 2683 *F \# *F anon-EST:Liang-2.42 = CG5014 *F >anon-EST:Liang-2.42 *F taagaaaaaa agagagacga gtaaagtaaa acgaaacagg cataaaaaca gcagcagttt *F tcttgatata tttggctaaa aaacgcaaac caaacagcca gcaagaacaa caaatagctg *F ggcaaaaaca ggacgcacaa aaaataaaat taaaacgata agaggcgaaa agcggagaga *F gtgaaattct cggcagcaac aacgacaaga acaacaccag gagcagcagc aacaacaaca *F acaacaaaag ccagccgcca caatgagcaa atcactcttt gatcttccgt tgaccattga *F accagaacat gagttgcgtt ttgtgggtcc cttcacccga cccgttgtca caatcatgac *F tctgcgcaac aactcggctc tgcctctggt cttcaagatc aagacaaccg ccccgaaacg *F ctactgcgta cgtccaaaca tcggcaagat aattcccttt cgatcaaccc aggtggagat *F ctgccttcag ccattcgtct acgattnagc aggagaagaa caagcacaag ttcatggtgc *F agagcgtcct ggcacccaat ggatgctgat ctaagcgatt taaataaatt gtggaaagga *F tctgganccc gnagcagctg gatgggacgn caaactgaaa gtgcgttttc nagatgnccc *F acngctgaag gcaaatgctg agaacaccaa cgggttggtg gtgccgttgg cggggggaac *F cgggagcaan gcngaaacct ctccat *F Database: dmel_all_transcript_r320 *F >CG5014-33-1-RC type=mRNA; *F loc=X:join(3690044..3690329,3692031..3692183, *F 3692252..3692361,3692445..3692657,3692724..3692837, *F 3693383..3694721); ID=Vap-33-1-RC; name=Vap-33-1-RC; *F db_xref='CG5014,FlyBase:FBgn0029687'; len=2215 *F Length = 2215 *F Genome Map *F Score = 712 bits (370), Expect = 0.0 *F Identities = 449/466 (96%), Gaps = 11/466 (2%) *F Strand = Plus / Plus *F Query: 248 aagccagccgccacaatgagcaaatcactctttgatcttccgttgaccattgaaccagaa 307 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208 aagccagccgccacaatgagcaaatcactctttgatcttccgttgaccattgaaccagaa 267 *F Query: 308 catgagttgcgttttgtgggtcccttcacccgacccgttgtcacaatcatgactctgcgc 367 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268 catgagttgcgttttgtgggtcccttcacccgacccgttgtcacaatcatgactctgcgc 327 *F Query: 368 aacaactcggctctgcctctggtcttcaagatcaagacaaccgccccgaaacgctactgc 427 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 328 aacaactcggctctgcctctggtcttcaagatcaagacaaccgccccgaaacgctactgc 387 *F Query: 428 gtacgtccaaacatcggcaagataattccctttcgatcaacccaggtggagatctgcctt 487 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 388 gtacgtccaaacatcggcaagataattccctttcgatcaacccaggtggagatctgcctt 447 *F Query: 488 cagccattcgtctacgattnagcaggagaagaacaagcacaagttcatggtgcagagcgt 547 *F ||||||||||||||||| | |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 448 cagccattcgtctacga-tcagcaggagaagaacaagcacaagttcatggtgcagagcgt 506 *F Query: 548 cctggcacccaatggatgctgatctaagcgatttaaataaattgtggaaaggatctggan 607 *F |||||||||| |||||||||||||||||||||||||||||||||||| ||||||||||| *F Sbjct: 507 cctggcaccc-atggatgctgatctaagcgatttaaataaattgtgg-aaggatctggag 564 *F Query: 608 cccgnagcagctggatgggacgncaaactgaaagtgcgttttcnagatgncccacngctg 667 *F |||| ||||||| ||| ||||| ||||||| |||||||||||| ||||| ||||| |||| *F Sbjct: 565 cccg-agcagct-gat-ggacgccaaactg-aagtgcgttttcgagatg-cccaccgctg 619 *F Query: 668 aaggcaaatgctgagaacaccaacgggttggtggtgccgttggcgg 713 *F ||||||||||||||||||||| ||| |||||||||||||||||| *F Sbjct: 620 \-aggcaaatgctgagaacaccagcgg--tggtggtgccgttggcgg 662 *F \# *F anon-EST:Liang-2.47 = CG1499 *F >anon-EST:Liang-2.47 *F gagttattcg gtcaactctc gacggtcaag ctcgcagcga tctcgcgact tggcaaccaa *F aaaataattc acaaaagcac cgaatcgcgt tacaaggaac tttagtccgc ggctttgtcg *F aggagcaacc tgccagcaaa gtcaagtgca acattcaata cccaccgatg tgcaatgtgt *F gtaatcagca aacagtgatc atttacatac atgaataaaa ctcatctcga aagtgaagcg *F tgactcagca tcgatagccg aaaaaggcag gagaacaaga caatccgaaa ggaaaggata *F cttttcggtc acttccaaaa ctcacgaccc caagttacag ttgcagttgc aaaatattct *F ggccaaatac aacgacgaca agacccgatc tgaagtttta gaggattgaa gacacgagag *F caaacagtta agacaaaatg tttctacgcc gctgcactct actgntgctg atntgcctaa *F ttgcgaatga cgccgccagc gccgcacgca aaacaaaagc gtcccgtcaa agccggtcaa *F acagancaat ccgcgcaaca atgtaacgcc ttcgccgccg ntgtcgcctc atcnggggta *F gttccacaac ccgccagaac cagnaagcac caccaacnac nggagggaan cnggngaacc *F aagaanggtg ggcanaatcc ggattttgca gccctanccg gggggcgggg aaaatgcccn *F aannctccgg ggnaagncca atcgnagggg ncaacccggg ggggaaa *F Database: dmel_all_transcript_r320 *F >CG1499-RA type=mRNA; *F loc=3R:join(27358120..27358615,27364375..27364440, *F 27373215..27373596,27374317..27374477, *F 27375090..27375291,27375622..27375873, *F 27375940..27376883,27377035..27377238, *F 27377318..27377401,27380199..27381255); ID=CG1499-RA; *F name=CG1499-RA; db_xref='CG1499,FlyBase:FBgn0039852'; *F len=3848 *F Length = 3848 *F Genome Map *F Score = 1063 bits (553), Expect = 0.0 *F Identities = 616/635 (97%), Gaps = 7/635 (1%) *F Strand = Plus / Plus *F Query: 2 agttattcggtcaactctcgacggtcaagctcgcagcgatctcgcgacttggcaaccaaa 61 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 132 agttattcggtcaactctcgacggtcaagctcgcagcgatctcgcgacttggcaaccaaa 191 *F Query: 62 aaataattcacaaaagcaccgaatcgcgttacaaggaactttagtccgcggctttgtcga 121 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 192 aaataattcacaaaagcaccgaatcgcgttacaaggaactttagtccgcggctttgacga 251 *F Query: 122 ggagcaacctgccagcaaagtcaagtgcaacattcaatacccaccgatgtgcaatgtgtg 181 *F |||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 252 ggagcaacctaccagcaaagtcaagtgcaacattcaatacccaccgatgtgcaatgtgtg 311 *F Query: 182 taatcagcaaacagtgatcatttacatacatgaataaaactcatctcgaaagtgaagcgt 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 312 taatcagcaaacagtgatcatttacatacatgaataaaactcatctcgaaagtgaagcgt 371 *F Query: 242 gactcagcatcgatagccgaaaaaggcaggagaacaagacaatccgaaaggaaaggatac 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 372 gactcagcatcgatagccgaaaaaggcaggagaacaagacaatccgaaaggaaaggatac 431 *F Query: 302 ttttcggtcacttccaaaactcacgaccccaagttacagttgcagttgcaaaatattctg 361 *F |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 432 ttttcggtcacttccagaactcacgaccccaagttacagttgcagttgcaaaatattctg 491 *F Query: 362 gccaaatacaacgacgacaagacccgatctgaagttttagaggattgaagacacgagagc 421 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 492 gccaaatacaacgacgacaagacccgatctgaagttttagaggattgaagacacgagagc 551 *F Query: 422 aaacagttaagacaaaatgtttctacgccgctgcactctactgntgctgatntgcctaat 481 *F ||||||||||||||||||||||||||||||||||||||||||| ||||||| |||||||| *F Sbjct: 552 aaacagttaagacaaaatgtttctacgccgctgcactctactgctgctgatctgcctaat 611 *F Query: 482 tgcgaatgacgccgccagcgccgcacgcaaaacaaaagcgtcccgtcaaagccggtcaaa 541 *F ||||| ||||||||||||||||||||||||||| ||||||||||||| ||||||||||| *F Sbjct: 612 tgcgagtgacgccgccagcgccgcacgcaaaac-aaagcgtcccgtc-aagccggtcaag 669 *F Query: 542 cagancaatccgcgcaacaatgtaacgccttcgccgcc-gntgtcgcctcatcnggggta 600 *F |||| ||||||||||| ||||||||||||||||||||| | |||||||||||| ||||| *F Sbjct: 670 cagaccaatccgcgcaccaatgtaacgccttcgccgccggctgtcgcctcatc--gggta 727 *F Query: 601 gttccacaacccgccagaaccagnaagcaccacca 635 *F ||||||| ||||||||| ||||| |||||||||| *F Sbjct: 728 gttccac-acccgccagcaccagc-agcaccacca 760 *F \# *F anon-EST:Liang-2.48 = CG14938 *F >anon-EST:Liang-2.48 *F aatcatgtac ggcaacatac aggcgagtcg ccgcacaagt gcacgtactg caccaagacg *F ttcacgcgca aggagcacct gacgaaccat gtgcgccagc acacgggcga ctccccgcac *F cgctgctcct actgcaagaa gaccttcacg cgcaaggagc acctgacgaa ccatgtgcgc *F ctgcacacgg gcgactcgcc gcacaagtgc gagtactgcc agaagacgtt tacgcggaag *F gagcacctga acaatcatat gcgccagcat tcgagcgaca atccgcattg ctgcaacgtt *F tgcaacaagc cgtttacgcg caaggaacac ctgatcaacc atatgtcgcg gtgccacacc *F ggtgaccggc ccttcacctg cgagacgtgc ggcaaatcgt tcccgctcaa gggcaatctg *F ctcttccatc agcgcagcca taccaagggc caggaagatg gagcggccat tcgcctgcga *F gaagtgcccc aagaacttca tctgcaaagg tcacttggtc tcgcacatgc gctcccattc *F gggtgagaaa ccacacgcgt gcacactgtg caacaaggng ttcgtcnagc cgcggcaatt *F tgaagcgcca catgaangat tgaatcaccc ggattgctat gntnccgnca ccaacccgtn *F gaatccgcat tccgcaaata ccggntggtt gtgctgacgc aagtcaagca nggaagtgaa *F aaccgattat tantttccca tcactctg *F Database: dmel_all_transcript_r320 *F >CG14938-RC type=mRNA; *F loc=2L:complement(11783198..11786612,11786700..11786912, *F 11787536..11788273,11789026..11789193,11791776..11792545, *F 11792656..11792769,11794919..11795028, *F 11796885..11797843); ID=crol-RC; name=crol-RC; *F db_xref='CG14938,FlyBase:FBgn0020309'; len=6487 *F Length = 6487 *F Genome Map *F Score = 1194 bits (621), Expect = 0.0 *F Identities = 720/746 (96%), Gaps = 12/746 (1%) *F Strand = Plus / Plus *F Query: 1 aatcatgtacggcaacatacaggcgagtcgccgcacaagtgcacgtactgcaccaagacg 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2352 aatcatgtacggcaacatacaggcgagtcgccgcacaagtgcacgtactgcaccaagacg 2411 *F Query: 61 ttcacgcgcaaggagcacctgacgaaccatgtgcgccagcacacgggcgactccccgcac 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2412 ttcacgcgcaaggagcacctgacgaaccatgtgcgccagcacacgggcgactccccgcac 2471 *F Query: 121 cgctgctcctactgcaagaagaccttcacgcgcaaggagcacctgacgaaccatgtgcgc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2472 cgctgctcctactgcaagaagaccttcacgcgcaaggagcacctgacgaaccatgtgcgc 2531 *F Query: 181 ctgcacacgggcgactcgccgcacaagtgcgagtactgccagaagacgtttacgcggaag 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2532 ctgcacacgggcgactcgccgcacaagtgcgagtactgccagaagacgtttacgcggaag 2591 *F Query: 241 gagcacctgaacaatcatatgcgccagcattcgagcgacaatccgcattgctgcaacgtt 300 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2592 gagcacctcaacaatcatatgcgccagcattcgagcgacaatccgcattgctgcaacgtt 2651 *F Query: 301 tgcaacaagccgtttacgcgcaaggaacacctgatcaaccatatgtcgcggtgccacacc 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2652 tgcaacaagccgtttacgcgcaaggaacacctgatcaaccatatgtcgcggtgccacacc 2711 *F Query: 361 ggtgaccggcccttcacctgcgagacgtgcggcaaatcgttcccgctcaagggcaatctg 420 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2712 ggtgaccggcccttcacctgcgagacgtgcggcaaatcgttcccgctcaagggcaatctg 2771 *F Query: 421 ctcttccatcagcgcagccataccaagggccaggaagatggagcggccattcgcctgcga 480 *F |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 2772 ctcttccatcagcgcagccataccaagggccagg-agatggagcggccattcgcctgcga 2830 *F Query: 481 gaagtgccccaagaacttcatctgcaaaggtcacttggtctcgcacatgcgctcccattc 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2831 gaagtgccccaagaacttcatctgcaaaggtcacttggtctcgcacatgcgctcccattc 2890 *F Query: 541 gggtgagaaaccacacgcgtgcacactgtgcaacaaggngttcgtcnagccgcggcaatt 600 *F |||||||||||||||||||||||||||||||| ||||| ||||||| || |||||||||| *F Sbjct: 2891 gggtgagaaaccacacgcgtgcacactgtgcagcaaggcgttcgtcgag-cgcggcaatt 2949 *F Query: 601 tgaagcgccacatgaangattgaatcacccggattgctatgntnccgncaccaacccgtn 660 *F |||||||||||||||| ||||||||||||| ||||||| | ||| |||| |||||| *F Sbjct: 2950 tgaagcgccacatgaag--atgaatcacccgga-tgctatgatgccgccacc-acccgt- 3004 *F Query: 661 gaatccgcattccgcaaataccggntggttgtgctgacgcaagtcaagcanggaagtgaa 720 *F | ||||||| |||||||||||||| ||| ||||||||||||||||||||| ||||||| | *F Sbjct: 3005 gcatccgca-tccgcaaataccggctgg-tgtgctgacgcaagtcaagca-ggaagtg-a 3060 *F Query: 721 aaccgattattantttcccatcactc 746 *F ||||||| | || || |||||||||| *F Sbjct: 3061 aaccgatca-taattccccatcactc 3085 *F \# *F anon-EST:Liang-2.49 = CG3821 *F >anon-EST:Liang-2.49 *F gaagtacacg acgctcgtga aatcaattcg tttgtgtatt aaaagggcaa gatctagctg *F ccgtttttgt gcattccaag atggtcgagg acaaagagca agtggccaac ggggagcagg *F tctccaagaa gggagccaaa aagctggcca aggccgctaa gaaagcgaac aaaaagcgga *F gaacgcctcc accgcggcgg ccaataacgc tggtggcgat tctgcggagg atcacgccgc *F cggaagatat ggcctctcgg aaatgatcca atccaaggac aaacgcagtg aacgcaactt *F tgtcccggtt tcggaactaa gcggtcaggt gggcaaaggg ctggtctggg tgcgangacg *F cgtccacact tcnagagcca agggaaagca ntgcttcctc atcctgcgcc agcagagcag *F cacggtgcan ttgcatcctg gctgtcngtg atgttatctc caaacanatg gtcnaatttn *F ccgggaaana tccctaaagg ggagcattan tggatatcca angncaaacc aaattgcggt *F gtctaacaaa nattgaatcc tgcacaaanc aatccccngg naatntccct tcaancanat *F attcnttaat atcccaagnc aaaggaacaa nttnncattt caaatccaag gntncatccc *F gttcccnnaa aanccccttt atnccggaaa gggtctgaaa attnccgtta accanganaa *F accccttgtg aaaaccnatt nttntacctt aaggaccccg ncaaattaag gccatttttc *F caccgggaaa ccngggtttt ttt *F Database: dmel_all_transcript_r320 *F >CG3821-asp-RA type=mRNA; *F loc=2R:join(7945033..7945094,7945484..7945584, *F 7945664..7945989,7946222..7947246,7947456..7947884); *F ID=Aats-asp-RA; name=Aats-asp-RA; *F db_xref='CG3821,FlyBase:FBgn0002069'; len=1943 *F Length = 1943 *F Genome Map *F Score = 881 bits (458), Expect = 0.0 *F Identities = 537/565 (95%), Gaps = 8/565 (1%) *F Strand = Plus / Plus *F Query: 3 agtacacgacgctcgtgaaatcaattcgtttgtgtattaaaagggcaagatctagctgcc 62 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5 agtacacgacgctcgtgaaatcaattcgtttgtgtattaaaagggcaagatctagctgcc 64 *F Query: 63 gtttttgtgcattccaagatggtcgaggacaaagagcaagtggccaacggggagcaggtc 122 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65 gtttttgtgcattccaagatggtcgaggacaaagagcaagtggccaacggggagcaggtc 124 *F Query: 123 tccaagaagggagccaaaaagctggccaaggccgctaagaaagc-gaacaaaaagcggag 181 *F |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 125 tccaagaagggagccaaaaagctggccaaggccgctaagaaagccgaacaaaaagcggag 184 *F Query: 182 aacgcctccaccgcggcggccaataacgctggtggcgattctgcggaggatcacgccgcc 241 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 185 aacgcctccaccgcggcggccaataacgctggtggcgattctgcggaggatcacgccgcc 244 *F Query: 242 ggaagatatggcctctcggaaatgatccaatccaaggacaaacgcagtgaacgcaacttt 301 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 245 ggaagatatggcctctcggaaatgatccaatccaaggacaaacgcagtgaacgcaacttt 304 *F Query: 302 gtcccggtttcggaactaagcggtcaggtgggcaaagggctggtctgggtgcgangacgc 361 *F |||||||||||||||||||||||||||||||||||||| ||||||||||||||| ||||| *F Sbjct: 305 gtcccggtttcggaactaagcggtcaggtgggcaaaggactggtctgggtgcgaggacgc 364 *F Query: 362 gtccacacttcnagagccaagggaaagcantgcttcctcatcctgcgccagcagagcagc 421 *F ||||||||||| ||||||||||||||||| |||||||||||||||||||||||||||||| *F Sbjct: 365 gtccacacttcgagagccaagggaaagcagtgcttcctcatcctgcgccagcagagcagc 424 *F Query: 422 acggtgcanttgcatcctggctgtcngtgatgttatctccaaacanatggtcnaatttnc 481 *F |||||||| ||||||||||||||| ||||||||||||||||||| |||||| ||||| *F Sbjct: 425 acggtgcag-tgcatcctggctgtcggtgatgttatctccaaacagatggtcaaatttg- 482 *F Query: 482 cgggaaanatccctaaaggggagcattantggatatccaangncaaaccaaattgcggtg 541 *F ||||||| |||||| |||| |||||||| | |||||||| | | || | | | |||||| *F Sbjct: 483 cgggaaatatccct-aaggagagcattatt-gatatcca--ggccaagccagtggcggtg 538 *F Query: 542 tctaacaaanattgaatcctgcaca 566 *F |||| | || ||||||||||||||| *F Sbjct: 539 tctagc-aagattgaatcctgcaca 562 *F \# *F anon-EST:Liang-2.51 == CG7533 (charybde) (1-635 only; chimeric? Last *F 150bp not identifiable \-- het?) *F \# *F anon-EST:Liang-2.54 == CG9894 *F \# *F anon-EST:Liang-20 == CG10035 *F \# *F anon-EST:Liang-38 == CG17579 (sca) *F \# *F anon-EST:Liang-97 == CG3359 (1-468 only; chimeric cDNA? Last 300bp not *F identifiable \-- het?) *F \# *F Date: Wed, 30 Jun 2004 18:23:22 \+0100 (BST) *F From: 'Michael Ashburner (Genetics)' *F Subject: Death to anon-ESTs *F To: michael@morgan.harvard.edu, crosby@morgan.harvard.edu *F Cc: gm119@gen.cam.ac.uk *F Some more \- will start on Poseys *F Michael *F anon-EST:Gibbs1 = ? maybe new *F anon-EST:Gibbs2 = ? maybe new *F anon-EST:GressD1 == ? maybe new or junk *F anon-EST:GressD10 = CG9253, I think (must be ?:) *F anon-EST:GressD11 = ?, only self match *F anon-EST:GressD16 = CG6483 *F anon-EST:GressD4 = CG4863 *F anon-EST:GressD5 = CG2934 *F anon-EST:GressD7 = CG6667 *F anon-EST:Nelson1 = CG4065 *F anon-EST:ParkEST125 = CG4182 *F anon-EST:ParkEST132 = CG10992 *F anon-EST:ParkEST325 = CG6199 *F =================================== *F anon-EST:Gibbs1 = ? maybe new *F >anon-EST:Gibbs1.1 *F gaattcggct tcgacgggac caccttatgt tatttcatca tggcctcgac gttcgctaac *F agagctgagc actgttaagc ggcgcttcgt gactgtgagc cgaacgcata cgaacgacct *F acgccagcgc gctcaaacct gttggccaac tgagtgtgca acaaacatgt tgtgtttacg *F ttttttcctt ggcctaagcg gtttgagcat gttgctgtcc tccatcaaca tgttgcactt *F ttcgggcttg gcaacaagtg ctttttgttt ccaacttcca gatactcttt ctatttaact *F gtatttatgg ttgtagttat attacgccgt taattgtgaa atgttaccaa tgagtattgc *F atataaaaat catttaaaat ttacatatta caaactcaag ctgattttat taaaattaaa *F tgtatatatc taagtcctat tcaaaaaaaa aacgtatcaa cagaagctgc gtaatatatt *F gcttaattca aattggacat tcagccgaaa taaaaatatc tttgacagat acactaggaa *F gctctgacac ggcgaaaagt aggacaaaat cggttcaggg gagatggtca ttgtgggtgg *F acgcgttcag cggggagctg taacttttct aactggcatt tttccgcaat gattgcagtt *F gaacacgctg cgataaaagc taatcgaatt atgcttttgg caaatgccac aacagctgca *F cgaaaccagc aaaaactaac aaacaattat gggtctctca actggggcgg ggaatgggaa *F aatcgaatag ggggagaatg gtcaagggtg gcttataact ctgctatgtg cggcgtcatg *F tcaacgccca gccaagcaaa gcgaggacca aaaggagacg cagcggtttg gagtttccgg *F caaaaagtta gtgggcaaag gaaagagcca aaaagggatt tgtgtatgcg aaaaaatttt *F acaagccatt aacaaaaatg ttgcgcctcg gcatatgcca cataaaattc actcagcagc *F agcagcagca gcagtggtaa caatggagga gtcaggcgcc taactcaggc acacacagat *F ggaaagcgag tggaacggtc ggaaaataag cggcataacc gcattcccaa tacgctatcc *F aaaatattat agccctagaa aagggtagca tttgctcttt catcagtaga gaattatgat *F tatttaagaa cagataaata attaaacttt ttataataaa tgttcaacta tcaatcgctg *F cagcttaata tatcttctgc atatattctt tcctcatggg caggtatgta catatatgaa *F tggagaaaac tcaattatcc tttgtgaggg tactgaaaag agcgaaacaa agagagagac *F tgatgcaaaa aggaaggagg ggcgaaaagc gagtcgaatg ccaaaatgtg caatggaag *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003679|gb|AE003679|arm:3R 4269603..4486596 *F estimated-cyto:85A1-85A5 gadfly-seqname:AE003679 *F seq_release:3 *F Length = 216994 *F Score = 2527 bits (1314), Expect = 0.0 Genome Map *F Identities = 1359/1397 (97%) *F Strand = Plus / Plus *F Query: 43 gcctcgacgttcgctaacagagctgagcactgttaagcggcgcttcgtgactgtgagccg 102 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 33811 gcctcgacgttcgctaacagagctgagcactgttaagcggcgcttcgtgactgtgagccg 33870 *F Query: 103 aacgcatacgaacgacctacgccagcgcgctcaaacctgttggccaactgagtgtgcaac 162 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 33871 aacgcatacgaacgacctacgccagcgcgctcaaacctgttggccaactgagtgtgcaac 33930 *F Query: 163 aaacatgttgtgtttacgttttttccttggcctaagcggtttgagcatgttgctgtcctc 222 *F |||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||| *F Sbjct: 33931 aaacatgttgtgtttacgttttttccttggcctaagcggtttgagcgtgttgctgtcctc 33990 *F Query: 223 catcaacatgttgcacttttcgggcttggcaacaagtgctttttgtttccaacttccaga 282 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 33991 catcaacatgttgcacttttcgggcttggcaacaagtgctttttgtttccaactaccaga 34050 *F Query: 283 tactctttctatttaactgtatttatggttgtagttatattacgccgttaattgtgaaat 342 *F |||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||| *F Sbjct: 34051 tactctttctatttaactgtatttatggttgtagttatattatgccgttaattgtgaaat 34110 *F Query: 343 gttaccaatgagtattgcatataaaaatcatttaaaatttacatattacaaactcaagct 402 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34111 gttaccaatgagtattgcatataaaaatcatttaaaatttacatattacaaactcaagct 34170 *F Query: 403 gattttattaaaattaaatgtatatatctaagtcctattcnnnnnnnnnncgtatcaaca 462 *F |||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 34171 gattttattaaaattaaatgtatatatctaagtcctattcaaaaaaaaaacgtatcaaca 34230 *F Query: 463 gaagctgcgtaatatattgcttaattcaaattggacattcagccgaaataaaaatatctt 522 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| || *F Sbjct: 34231 gaagctgcgtaatatattgcttaattcaaattggacattcagccgaaataaaaatatttt 34290 *F Query: 523 tgacagatacactaggaagctctgacacggcgaaaagtaggacaaaatcggttcagggga 582 *F ||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 34291 tgacagatacactaggaagctctgacacggcaaaaagtaggacaaaatcggttcagggga 34350 *F Query: 583 gatggtcattgtgggtggacgcgttcagcggggagctgtaacttttctaactggcatttt 642 *F |||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 34351 gatggtcattgtgggtggacgcgttcagcggggagctgtaacttttctaattggcatttt 34410 *F Query: 643 tccgcaatgattgcagttgaacacgctgcgataaaagctaatcgaattatgcttttggca 702 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34411 tccgcaatgattgcagttgaacacgctgcgataaaagctaatcgaattatgcttttggca 34470 *F Query: 703 aatgccacaacagctgcacgaaaccagcaaaaactaacaaacaattatgggtctctcaac 762 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34471 aatgccacaacagctgcacgaaaccagcaaaaactaacaaacaattatgggtctctcaac 34530 *F Query: 763 tggggcggggaatgggaaaatcgaatagggggagaatggtcaagggtggcttataactct 822 *F ||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 34531 tggggcggggaatgggaaaatcgaatagggggagaatgggcaagggtggcttataactct 34590 *F Query: 823 gctatgtgcggcgtcatgtcaacgcccagccaagcaaagcgaggaccaaaaggagacgca 882 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34591 gctatgtgcggcgtcatgtcaacgcccagccaagcaaagcgaggaccaaaaggagacgca 34650 *F Query: 883 gcggtttggagtttccggcaaaaagttagtgggcaaaggaaagagccaaaaagggatttg 942 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34651 gcggtttggagtttccggcaaaaagttagtgggcaaaggaaagagccaaaaagggatttg 34710 *F Query: 943 tgtatgcgaaaaaattttacaagccattaacaaaaatgttgcgcctcggcatatgccaca 1002 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34711 tgtatgcgaaaaaattttacaagccattaacaaaaatgttgcgcctcggcatatgccaca 34770 *F Query: 1003 taaaattcactnnnnnnnnnnnnnnnnnnnnntggtaacaatggaggagtcaggcgccta 1062 *F ||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 34771 taaaattcactcagcagcagcagcagcagcagtggtaacaatggaggagtcaggcgccta 34830 *F Query: 1063 actcaggcacacacagatggaaagcgagtggaacggtcggaaaataagcggcataaccgc 1122 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34831 actcaggcacacacagatggaaagcgagtggaacggtcggaaaataagcggcataaccgc 34890 *F Query: 1123 attcccaatacgctatccaaaatattatagccctagaaaagggtagcatttgctctttca 1182 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34891 attcccaatacgctatccaaaatattatagccctagaaaagggtagcatttgctctttca 34950 *F Query: 1183 tcagtagagaattatgattatttaagaacagataaataattaaactttttataataaatg 1242 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34951 tcagtagagaattatgattatttaagaacagataaataattaaactttttataataaatg 35010 *F Query: 1243 ttcaactatcaatcgctgcagcttaatatatcttctgcatatattctttcctcatgggca 1302 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35011 ttcaactatcaatcgctgcagcttaatatatcttctgcatatattctttcctcatgggca 35070 *F Query: 1303 ggtatgtacatatatgaatggagaaaactcaattatcctttgtgagggtactgaaaagag 1362 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35071 ggtatgtacatatatgaatggagaaaactcaattatcctttgtgagggtactgaaaagag 35130 *F Query: 1363 cgaaacaaagagagagactgatgcaaaaaggaaggaggggcgaaaagcgagtcgaatgcc 1422 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35131 cgaaacaaagagagagactgatgcaaaaaggaaggaggggcgaaaagcgagtcgaatgcc 35190 *F Query: 1423 aaaatgtgcaatggaag 1439 *F ||||||||||||||||| *F Sbjct: 35191 aaaatgtgcaatggaag 35207 *F >anon-EST:Gibbs1.2 *F gcctcgacgt tcgctaacag agctgagcac tgttaagcgg cgcttcgtga ctgtgagccg *F aacgcatacg aacgacctac gccagcgcgc tcaaacctgt tggccaactg agtgtgcaac *F aaacatgttg tgtttacgtt ttttccttgg cctaagcggt ttgagcgtgt tgctgtcctc *F catcaacatg ttgcactttt cgggcttggc aacaagtgct ttttgtttcc aactaccaga *F tactctttct atttaactgt atttatggtt gtagttatat tatgccgtta attgtgaaat *F gttaccaatg agtattgcat ataaaaatca tttaaaattt acatattaca aactcaagct *F gattttatta aaattaaatg tatatatcta agtcctattc aaaaaaaaaa cgtatcaaca *F gaagctgcgt aatatattgc ttaattcaaa ttggacattc agcccgaata aaatattttt *F gacagatcac taggaagctc tgacacggaa aaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003679|gb|AE003679|arm:3R 4269603..4486596 *F estimated-cyto:85A1-85A5 gadfly-seqname:AE003679 *F seq_release:3 *F Length = 216994 *F Score = 904 bits (470), Expect = 0.0 Genome Map *F Identities = 500/515 (97%), Gaps = 2/515 (0%) *F Strand = Plus / Plus *F Query: 1 gcctcgacgttcgctaacagagctgagcactgttaagcggcgcttcgtgactgtgagccg 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 33811 gcctcgacgttcgctaacagagctgagcactgttaagcggcgcttcgtgactgtgagccg 33870 *F Query: 61 aacgcatacgaacgacctacgccagcgcgctcaaacctgttggccaactgagtgtgcaac 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 33871 aacgcatacgaacgacctacgccagcgcgctcaaacctgttggccaactgagtgtgcaac 33930 *F Query: 121 aaacatgttgtgtttacgttttttccttggcctaagcggtttgagcgtgttgctgtcctc 180 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 33931 aaacatgttgtgtttacgttttttccttggcctaagcggtttgagcgtgttgctgtcctc 33990 *F Query: 181 catcaacatgttgcacttttcgggcttggcaacaagtgctttttgtttccaactaccaga 240 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 33991 catcaacatgttgcacttttcgggcttggcaacaagtgctttttgtttccaactaccaga 34050 *F Query: 241 tactctttctatttaactgtatttatggttgtagttatattatgccgttaattgtgaaat 300 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34051 tactctttctatttaactgtatttatggttgtagttatattatgccgttaattgtgaaat 34110 *F Query: 301 gttaccaatgagtattgcatataaaaatcatttaaaatttacatattacaaactcaagct 360 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 34111 gttaccaatgagtattgcatataaaaatcatttaaaatttacatattacaaactcaagct 34170 *F Query: 361 gattttattaaaattaaatgtatatatctaagtcctattcnnnnnnnnnncgtatcaaca 420 *F |||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 34171 gattttattaaaattaaatgtatatatctaagtcctattcaaaaaaaaaacgtatcaaca 34230 *F Query: 421 gaagctgcgtaatatattgcttaattcaaattggacattcagcccgaat-aaaatatttt 479 *F |||||||||||||||||||||||||||||||||||||||||||| ||| |||||||||| *F Sbjct: 34231 gaagctgcgtaatatattgcttaattcaaattggacattcagccgaaataaaaatatttt 34290 *F Query: 480 tgacagat-cactaggaagctctgacacggaaaaa 513 *F |||||||| ||||||||||||||||||||| |||| *F Sbjct: 34291 tgacagatacactaggaagctctgacacggcaaaa 34325 *F \# *F anon-EST:Gibbs2 = ? maybe new *F >anon-EST:Gibbs2 *F gaattccata atttataatc cttgctctgg acccccagca acaataatac caacaacaac *F aacaacaaca acaacaacaa caacagcagc agcagcaaca agaacaaaca cagcaacagg *F agcaggagta acaagagcag aaataatatg ggaatgccag tgcaaatatt tttgcttgca *F cctctcggtt tctatgatcc gagtttcctt ttacttttat gtcctatttt atttattcga *F aacctgtgcc tctcccaggt tggcagcaca aatattttgt tggttccctt ccgagccacc *F cagccactat ttcacacccg ctcggctgca tttagtttat gtgtgtgtta ccttttttct *F cggtaaaatg aggtcaaaag tttattggct aataaatgtg tgcgcaagtg ggtggggtgt *F tgttttcgca ggcattaatg tcaggcgcaa actgcagagg ctgcaaagtt tttggcagag *F ctttaatgcc gataaacaaa ttgcatgcac ttagctacag gccgcccatc caaaaggaga *F tgggcaggat tagggcagtc gaaatgggct ttaaatgatc ctgcagcaca ctcgatggca *F cgatggccac aaaagcgaat cagctgccct accgacggct aattacaaac taatcagcca *F acaaataaat ttcaaataaa ttatacaaga acggaattgg ctaatgcatt gcagttgaac *F gacagccaga gcgaaacaaa aaacttcaaa tctatgttgg gccaaccaaa ccgaacgaaa *F gcaaaccaaa ccaagccaaa actgccaaac acaccaaaat tttaagctat tcaattatgg *F ttcgttcaaa aagaacagca aaactatttt aattatttac attaagcccc tcaacatgtt *F tggccgacac acaagctctt ttctccaatt aaactgggaa taggatctag ctggcaaggg *F aaaccggcat agaatatctc attcgcattc acattcgact gaacatcaac cgatgtgctg *F taattcagaa cgggccgaaa gattcgagtt tcctctgatc agcccaaggt gaacttatag *F gcccccactc cctagaagaa ctaagttgtt cgccgtaaaa gcatttggtt caatgtttaa *F tgagggtggg ctcaataata gagaaagcct accttttatg cttgcctctt cccccgctgg *F gaaaacaacc ccttccaacg aataatgatt atgacagctt ctttgccgat gagcgactct *F gctcctgtca tttcgcactg gtttccactg tacctctgcc agatttgctg gctgcttggg *F ctttaattaa acggcaattg tggtcatcag ttgtcaggtt ctcttgtgcc taatcagttt *F gtgtgatgaa agactaagct gggattacgg aatgtctgcc atgcgggtgc gacatttgaa *F taatgaatgt caactcgatg ttcggcttgc aggggaaaaa tataagcccg acctaagtcc *F caaatgtcac tcagctgtca attgtaagca aatctaagga gcagagattc gggcggtttc *F gctagcggag gaggccaact aattggggcg tggccggttt cggccagctg acaaactaac *F tgaccaacta actgacgtaa ctaggcaagg caattggcct gcccgttagt ccgcccctcc *F atttgttcat ttgtccatgt cagttagctt ggccttctgt gtgcttgctg cttgaccata *F aactaatgca cctaattgct gtgattatgt cgtttgtgat tatctaagtg tgcccccgtg *F tccttgtccc cgtttccgtc ttctggagag ttctgtgcct ctgattttgc cattgcaatg *F cattgaccgc ctgcatgcgc tgcatacaat taggcacaaa acctaaatct catttatcaa *F tcacgagtcc tccggcgttc ggcgcgataa tatttgataa ttggccaaag ccattactga *F tagtcccagc tggatcagaa ttc *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003597|gb|AE003597|arm:3L 22100943..22406554 *F estimated-cyto:79D1-79E5 gadfly-seqname:AE003597 *F seq_release:3 *F Length = 305612 *F Score = 3584 bits (1864), Expect = 0.0 Genome Map *F Identities = 1878/1885 (99%) *F Strand = Plus / Plus *F Query: 119 ggagcaggagtaacaagagcagaaataatatgggaatgccagtgcaaatatttttgcttg 178 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 147665 ggagcaggagtaacaagagcagaaataatatgggaatgccagtgcaaatatttttgcttg *F 147724 *F Query: 179 cacctctcggtttctatgatccgagtttccttttacttttatgtcctattttatttattc 238 *F | |||||||||||||||||||||||||||||||||||||| ||||||||||||||||||| *F Sbjct: 147725 ctcctctcggtttctatgatccgagtttccttttacttttttgtcctattttatttattc *F 147784 *F Query: 239 gaaacctgtgcctctcccaggttggcagcacaaatattttgttggttcccttccgagcca 298 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 147785 gaaacctgtgcctctcccaggttggcagcacaaatattttgttggttcccttccgagcca *F 147844 *F Query: 299 cccagccactatttcacacccgctcggctgcatttagtttatgtgtgtgttacctttttt 358 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 147845 cccagccactatttcacacccgctcggctgcatttagtttatgtgtgtgttacctttttt *F 147904 *F Query: 359 ctcggtaaaatgaggtcaaaagtttattggctaataaatgtgtgcgcaagtgggtggggt 418 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 147905 ctcggtaaaatgaggtcaaaagtttattggctaataaatgtgtgcgcaagtgggtggggt *F 147964 *F Query: 419 gttgttttcgcaggcattaatgtcaggcgcaaactgcagaggctgcaaagtttttggcag 478 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 147965 gttgttttcgcaggcattaatgtcaggcgcaaactgcagaggctgcaaagtttttggcag *F 148024 *F Query: 479 agctttaatgccgataaacaaattgcatgcacttagctacaggccgcccatccaaaagga 538 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| *F Sbjct: 148025 agctttaatgccgataaacaaattgcatgcacttagctacaggccgcccatccaagggga *F 148084 *F Query: 539 gatgggcaggattagggcagtcgaaatgggctttaaatgatcctgcagcacactcgatgg 598 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148085 gatgggcaagattagggcagtcgaaatgggctttaaatgatcctgcagcacactcgatgg *F 148144 *F Query: 599 cacgatggccacaaaagcgaatcagctgccctaccgacggctaattacaaactaatcagc 658 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148145 cacgatggccacaaaagcgaatcagctgccctaccgacggctaattacaaactaatcagc *F 148204 *F Query: 659 caacaaataaatttcaaataaattatacaagaacggaattggctaatgcattgcagttga 718 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148205 caacaaataaatttcaaataaattatacaagaacggaattggctaatgcattgcagttga *F 148264 *F Query: 719 acgacagccagagcgaaacaaaaaacttcaaatctatgttgggccaaccaaaccgaacga 778 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148265 acgacagccagagcgaaacaaaaaacttcaaatctatgttgggccaaccaaaccgaacga *F 148324 *F Query: 779 aagcaaaccaaaccaagccaaaactgccaaacacaccaaaattttaagctattcaattat 838 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148325 aagcaaaccaaaccaagccaaaactgccaaacacaccaaaattttaagctattcaattat *F 148384 *F Query: 839 ggttcgttcaaaaagaacagcaaaactattttaattatttacattaagcccctcaacatg 898 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148385 ggttcgttcaaaaagaacagcaaaactattttaattatttacattaagcccctcaacatg *F 148444 *F Query: 899 tttggccgacacacaagctcttttctccaattaaactgggaataggatctagctggcaag 958 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148445 tttggccgacacacaagctcttttctccaattaaactgggaataggatctagctggcaag *F 148504 *F Query: 959 ggaaaccggcatagaatatctcattcgcattcacattcgactgaacatcaaccgatgtgc 1018 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148505 ggaaaccggcatagaatatctcattcgcattcacattcgactgaacatcaaccgatgtgc *F 148564 *F Query: 1019 tgtaattcagaacgggccgaaagattcgagtttcctctgatcagcccaaggtgaacttat 1078 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148565 tgtaattcagaacgggccgaaagattcgagtttcctctgatcagcccaaggtgaacttat *F 148624 *F Query: 1079 aggcccccactccctagaagaactaagttgttcgccgtaaaagcatttggttcaatgttt 1138 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148625 aggcccccactccctagaagaactaagttgttcgccgtaaaagcatttggttcaatgttt *F 148684 *F Query: 1139 aatgagggtgggctcaataatagagaaagcctaccttttatgcttgcctcttcccccgct 1198 *F |||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||| *F Sbjct: 148685 aatgagggtgggctcaataatacagaaagcctaccttttatgcttgcctcttcccccgct *F 148744 *F Query: 1199 gggaaaacaaccccttccaacgaataatgattatgacagcttctttgccgatgagcgact 1258 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148745 gggaaaacaaccccttccaacgaataatgattatgacagcttctttgccgatgagcgact *F 148804 *F Query: 1259 ctgctcctgtcatttcgcactggtttccactgtacctctgccagatttgctggctgcttg 1318 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148805 ctgctcctgtcatttcgcactggtttccactgtacctctgccagatttgctggctgcttg *F 148864 *F Query: 1319 ggctttaattaaacggcaattgtggtcatcagttgtcaggttctcttgtgcctaatcagt 1378 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148865 ggctttaattaaacggcaattgtggtcatcagttgtcaggttctcttgtgcctaatcagt *F 148924 *F Query: 1379 ttgtgtgatgaaagactaagctgggattacggaatgtctgccatgcgggtgcgacatttg 1438 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148925 ttgtgtgatgaaagactaagctgggattacggaatgtctgccatgcgggtgcgacatttg *F 148984 *F Query: 1439 aataatgaatgtcaactcgatgttcggcttgcaggggaaaaatataagcccgacctaagt 1498 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148985 aataatgaatgtcaactcgatgttcggcttgcaggggaaaaatataagcccgacctaagt *F 149044 *F Query: 1499 cccaaatgtcactcagctgtcaattgtaagcaaatctaaggagcagagattcgggcggtt 1558 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149045 cccaaatgtcactcagctgtcaattgtaagcaaatctaaggagcagagattcgggcggtt *F 149104 *F Query: 1559 tcgctagcggaggaggccaactaattggggcgtggccggtttcggccagctgacaaacta 1618 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149105 tcgctagcggaggaggccaactaattggggcgtggccggtttcggccagctgacaaacta *F 149164 *F Query: 1619 actgaccaactaactgacgtaactaggcaaggcaattggcctgcccgttagtccgcccct 1678 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149165 actgaccaactaactgacgtaactaggcaaggcaattggcctgcccgttagtccgcccct *F 149224 *F Query: 1679 ccatttgttcatttgtccatgtcagttagcttggccttctgtgtgcttgctgcttgacca 1738 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149225 ccatttgttcatttgtccatgtcagttagcttggccttctgtgtgcttgctgcttgacca *F 149284 *F Query: 1739 taaactaatgcacctaattgctgtgattatgtcgtttgtgattatctaagtgtgcccccg 1798 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149285 taaactaatgcacctaattgctgtgattatgtcgtttgtgattatctaagtgtgcccccg *F 149344 *F Query: 1799 tgtccttgtccccgtttccgtcttctggagagttctgtgcctctgattttgccattgcaa 1858 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149345 tgtccttgtccccgtttccgtcttctggagagttctgtgcctctgattttgccattgcaa *F 149404 *F Query: 1859 tgcattgaccgcctgcatgcgctgcatacaattaggcacaaaacctaaatctcatttatc 1918 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149405 tgcattgaccgcctgcatgcgctgcatacaattaggcacaaaacctaaatctcatttatc *F 149464 *F Query: 1919 aatcacgagtcctccggcgttcggcgcgataatatttgataattggccaaagccattact 1978 *F |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149465 aatcacgagtcctccgccgttcggcgcgataatatttgataattggccaaagccattact *F 149524 *F Query: 1979 gatagtcccagctggatcagaattc 2003 *F ||||||||||||||||||||||||| *F Sbjct: 149525 gatagtcccagctggatcagaattc 149549 *F \# *F anon-EST:GressD1 == ? maybe new or junk *F >anon-EST:GressD1 *F ttttctccaa atttcaaggg tctcaaatcg gattttttaa acaaaacctc gaaaaaatac *F tcaaaaactg aattgaccgg tgattaaaat ggaccgcctt tacaagacga gtggagtcgc *F ggacgatcta atggaccgat gtcgattaac gcgacaccca gcggcgaggg cagatcgcac *F ggcg *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003816|gb|AE003816|arm:2R 8923493..9210836 *F estimated-cyto:50C6-50E1 gadfly-seqname:AE003816 *F seq_release:3 *F Length = 287344 *F Score = 123 bits (64), Expect = 1e-27 Genome Map *F Identities = 89/94 (94%), Gaps = 3/94 (3%) *F Strand = Plus / Plus *F Query: 1 ttttctccaaatttcaagggtctcaaatcggattttttaaacaaaacctcgaaaaaatac 60 *F |||||||||||||||| | ||||||||||| ||||||||||||||||||||||||| ||| *F Sbjct: 3234 ttttctccaaatttcaggtgtctcaaatcg-attttttaaacaaaacctcgaaaaa-tac 3291 *F Query: 61 tcaaaaactgaattgaccggtgattaaaatggac 94 *F ||||||||||||||||||||||| |||||||||| *F Sbjct: 3292 tcaaaaactgaattgaccggtga-taaaatggac 3324 *F >gadfly|SEG:AE003441|gb|AE003441|arm:X 7253939..7539888 *F estimated-cyto:7B3-7B8 gadfly-seqname:AE003441 *F seq_release:3 *F Length = 285950 *F Score = 37.2 bits (19), Expect = 0.13 Genome Map *F Identities = 19/19 (100%) *F Strand = Plus / Minus *F Query: 24 caaatcggattttttaaac 42 *F ||||||||||||||||||| *F Sbjct: 80234 caaatcggattttttaaac 80216 *F Score = 31.5 bits (16), Expect = 7.2 Genome Map *F Identities = 18/19 (94%) *F Strand = Plus / Plus *F Query: 28 tcggattttttaaacaaaa 46 *F ||||| ||||||||||||| *F Sbjct: 79134 tcggactttttaaacaaaa 79152 *F \# *F anon-EST:GressD10 = CG9253, I think (must be ?:) *F >anon-EST:GressD10 *F tacaaacgag tgacgaaggg aggatctagg tagcggagcc aggaaatgaa gataacaatc *F ataaggaagg ggacagcagc actaagtgtg aagatgataa atccgaggat gaccgaggaa *F cagaagctaa cctggaagat ctggtctcaa tgaggcttgt gtcagcatgt gacgaattga *F agtgga *F Query= anon-EST:GressD10 *F >CG9253-RA type=mRNA; *F loc=2L:join(21112976..21113247,21113308..21113566, *F 21113756..21114895); ID=CG9253-RA; name=CG9253-RA; *F db_xref='CG9253,FlyBase:FBgn0032919'; len=1671 *F Length = 1671 *F Genome Map *F Score = 135 bits (70), Expect = 1e-31 *F Identities = 180/210 (85%), Gaps = 24/210 (11%) *F Strand = Plus / Plus *F Query: 1 tacaaacgagtgacgaag-ggaggatctaggtagcg-----gagccaggaaatgaagata 54 *F |||||||||||||||||| ||||||||||||||||| |||| | | ||||||||| *F Sbjct: 109 tacaaacgagtgacgaagaggaggatctaggtagcgaggaggagcaggaagatgaagata 168 *F Query: 55 acaatcataaggaaggggacagca-----gcactaagtg-tgaagatgataaa---tccg 105 *F ||||||||||||||||||||||| |||||||||| ||||||||||||| |||| *F Sbjct: 169 acaatcataaggaaggggacagcgaggcggcactaagtggtgaagatgataaaggctccg 228 *F Query: 106 aggatgac-----cgaggaacagaagctaacctgg-aagatct-ggtctcaatgaggc-t 157 *F |||| ||| |||||||||||||||||||||| ||||||| |||||||||||||| | *F Sbjct: 229 aggacgacgcagccgaggaacagaagctaacctggaaagatctcggtctcaatgaggctt 288 *F Query: 158 tgtgtc-agcatgtgacgaattgaagtgga 186 *F |||||| ||||||||||||||||||||||| *F Sbjct: 289 tgtgtcaagcatgtgacgaattgaagtgga 318 *F \# *F anon-EST:GressD11 = ?, only self match *F \*z FBgn0025330 *F \*g X65274 *F >anon-EST:GressD11 *F gaaataccca gtagataaaa ataaaacaga agtaaaaggc tgcataaaaa ataaattcca *F attgaagctc tagcaagttc acttcgtttt cgatacgagt gacaaaaaga gaccacatat *F Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, *F GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) 2,262,611 *F sequences; 10,883,439,008 total letters *F If you have any problems or questions with the results of this search *F please refer to the BLAST FAQs *F \# *F anon-EST:GressD16 = CG6483 *F >anon-EST:GressD16 *F ccctggctct ggccagcctc cgccggtctg ctgccccagc gagtgccgat ccacccccgt *F gacctgcccg ccgtgaccaa gatcgagggt cgcatcacca atggcaagac cgccacttct *F ggccagttcc ctaccaggtg ggactcagct tcgccagcac c *F Database: dmel_all_transcript_r320 *F >CG6483-RA type=mRNA; *F loc=3L:join(6010082..6010344,6010404..6011008); Genome Map *F ID=CG6483-RA; name=CG6483-RA; *F db_xref='CG6483,FlyBase:FBgn0035665'; len=868 *F Length = 868 *F Score = 256 bits (133), Expect = 4e-68 *F Identities = 157/164 (95%), Gaps = 3/164 (1%) *F Strand = Plus / Plus *F Query: 1 ccctggctctggcca--gcctccgccggtctgctgccccagcgagtgccgatccaccccc 58 *F ||||||||||||||| ||||||||||||||||||||||||| |||||||||||||||| *F Sbjct: 26 ccctggctctggccaccgcctccgccggtctgctgccccagcaggtgccgatccaccccc 85 *F Query: 59 gtgacctgcccgccgtgaccaagatcgagggtcgcatcaccaatggcaagaccgccactt 118 *F |||||||||||||||||||||| |||||||||||||||||||| |||||||||||||||| *F Sbjct: 86 gtgacctgcccgccgtgaccaatatcgagggtcgcatcaccaacggcaagaccgccactt 145 *F Query: 119 ctggccagtt-ccctaccaggtgggactcagcttcgccagcacc 161 *F |||||||||| ||||||||||||||||||||||||||||||||| *F Sbjct: 146 ctggccagttcccctaccaggtgggactcagcttcgccagcacc 189 *F \# *F anon-EST:GressD4 = CG4863 *F >anon-EST:GressD4 *F agcgatcaat aaacagattt tgggctaagc tgactcgaag atgcatctaa tggaaaagac *F cacgtacaga tttacatcga tatgtgatag agtgatcata tagtatctca t *F Database: dmel_all_transcript_r320 *F >CG4863-RB type=mRNA; *F loc=3R:join(7047909..7048107,7048301..7048469, *F 7049135..7049270,7049598..7049987,7050231..7050915); *F ID=RpL3-RB; name=RpL3-RB; *F db_xref='CG4863,FlyBase:FBgn0020910'; len=1579 *F Length = 1579 *F Genome Map *F Score = 54.5 bits (28), Expect = 2e-07 *F Identities = 35/36 (97%), Gaps = 1/36 (2%) *F Strand = Plus / Plus *F Query: 1 agcgatcaataaacagattttgggctaagctgactc 36 *F ||||||||||||||||||||| |||||||||||||| *F Sbjct: 1318 agcgatcaataaacagatttt-ggctaagctgactc 1352 *F Score = 35.3 bits (18), Expect = 0.093 *F Identities = 32/34 (94%), Gaps = 2/34 (5%) *F Strand = Plus / Plus *F Query: 42 tgcatctaatgg-aaaagaccacgt-acagattt 73 *F |||||||||||| |||||||||||| |||||||| *F Sbjct: 1368 tgcatctaatgggaaaagaccacgtcacagattt 1401 *F >CG4863-RE type=mRNA; *F loc=3R:join(7047659..7048107,7048301..7048469, *F 7049135..7049270,7049598..7049987,7050231..7050915); *F ID=RpL3-RE; name=RpL3-RE; *F db_xref='CG4863,FlyBase:FBgn0020910'; len=1829 *F Length = 1829 *F Genome Map *F Score = 54.5 bits (28), Expect = 2e-07 *F Identities = 35/36 (97%), Gaps = 1/36 (2%) *F Strand = Plus / Plus *F Query: 1 agcgatcaataaacagattttgggctaagctgactc 36 *F ||||||||||||||||||||| |||||||||||||| *F Sbjct: 1568 agcgatcaataaacagatttt-ggctaagctgactc 1602 *F Score = 35.3 bits (18), Expect = 0.093 *F Identities = 32/34 (94%), Gaps = 2/34 (5%) *F Strand = Plus / Plus *F Query: 42 tgcatctaatgg-aaaagaccacgt-acagattt 73 *F |||||||||||| |||||||||||| |||||||| *F Sbjct: 1618 tgcatctaatgggaaaagaccacgtcacagattt 1651 *F \# *F anon-EST:GressD5 = CG2934 *F >anon-EST:GressD5 *F ccacacaatt cgcgcaactt tcggtcgagc gagagcagtc ccatagaaag gaatccagcg *F caattcgcca cctcttcaac ttcagtcagc tcctctgcgt gtgtgtgtgt gcgtgtgtca *F tcaagatggc ttcagtcagc tcctctgcgt gtgtgtgtgt gcgtgtgtca tcaagatg *F Database: dmel_all_transcript_r320 *F >CG2934-RA type=mRNA; *F loc=X:join(3621356..3621731,3622070..3622578, *F 3622669..3623587); ID=VhaAC39-RA; name=VhaAC39-RA; *F db_xref='CG2934,FlyBase:FBgn0028665'; len=1804 *F Length = 1804 *F Genome Map *F Score = 175 bits (91), Expect = 9e-44 *F Identities = 98/99 (98%), Gaps = 1/99 (1%) *F Strand = Plus / Plus *F Query: 1 ccacacaattcgcgcaactttcggtcgagcgagagcagtcccatagaaaggaatccagcg 60 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 124 ccacacaattcgcgcaactttcggtcgagcgagagcagtcccatagaaaggaatccagcg 183 *F Query: 61 caattcgccacctcttcaactt-cagtcagctcctctgc 98 *F |||||||||||||||||||||| |||||||||||||||| *F Sbjct: 184 caattcgccacctcttcaacttccagtcagctcctctgc 222 *F Score = 31.5 bits (16), Expect = 2.3 *F Identities = 16/16 (100%) *F Strand = Plus / Plus *F Query: 133 cagtcagctcctctgc 148 *F |||||||||||||||| *F Sbjct: 207 cagtcagctcctctgc 222 *F \# *F anon-EST:GressD7 = CG6667 *F >anon-EST:GressD7 *F aaaagaaggc tgcataaaaa cggcataaat tcaattgaac gtctaaagca agtcacttgc *F tttttt *F Database: dmel_all_transcript_r320 *F >CG6667-RC type=mRNA; *F loc=2L:complement(17416681..17419962,17420023..17420417, *F 17420487..17420665,17420735..17421088, *F 17427379..17427451,17427959..17428442); ID=dl-RC; *F name=dl-RC; db_xref='CG6667,FlyBase:FBgn0000462'; *F len=4767 *F Length = 4767 *F Genome Map *F Score = 43.0 bits (22), Expect = 2e-04 *F Identities = 29/30 (96%), Gaps = 1/30 (3%) *F Strand = Plus / Plus *F Query: 9 gctgcataaaaacggcataaattcaattga 38 *F |||||||||||||| ||||||||||||||| *F Sbjct: 187 gctgcataaaaacg-cataaattcaattga 215 *F >CG6667-RB type=mRNA; *F loc=2L:complement(17414916..17415406,17415502..17416100, *F 17416233..17416295,17417273..17417380, *F 17419884..17419962,17420023..17420417, *F 17420487..17420665,17420735..17421088, *F 17427379..17427451,17427959..17428442); ID=dl-RB; *F name=dl-RB; db_xref='CG6667,FlyBase:FBgn0000462'; *F len=2825 *F Length = 2825 *F Genome Map *F Score = 43.0 bits (22), Expect = 2e-04 *F Identities = 29/30 (96%), Gaps = 1/30 (3%) *F Strand = Plus / Plus *F Query: 9 gctgcataaaaacggcataaattcaattga 38 *F |||||||||||||| ||||||||||||||| *F Sbjct: 187 gctgcataaaaacg-cataaattcaattga 215 *F \# *F anon-EST:Nelson1 = CG4065 *F >anon-EST:Nelson1 *F ggcangaggc gaaccagaga angggtctga cnctcccaat cccatgggtt tctctccgcg *F catccacgac cgcagccnac cgcccgcgtt tccgcgtacc attaagatca gggatcgtcc *F atccngttat cnatttctag aggaaatgat ttcgngcttc aaatacncct gcaaagtcnc *F cnnntacaag gattactant cggcgctgaa cttctttatc gantacagca aaaagtnggg *F ccactgcatc ctgtccagaa gcgtgctgca aaccctgttc agcgcccaca tgngttatgg *F cgcacngtaa agcttcccat naaagcantt ccngcgccat cggttcaggt cttcaactcn *F ccacccgtat tgaatgccaa ncacccngtg gctgcccatc ccaacgtgc *F Database: dmel_all_transcript_r320 *F >CG4065-RA type=mRNA; *F loc=2R:complement(19178741..19178935,19178998..19180035, *F 19180094..19180275,19180336..19180903,19180967..19181339, *F 19181429..19181606); ID=CG4065-RA; name=CG4065-RA; *F db_xref='CG4065,FlyBase:FBgn0034982'; len=2534 *F Length = 2534 *F Genome Map *F Score = 581 bits (302), Expect = e-165 *F Identities = 366/399 (91%), Gaps = 4/399 (1%) *F Strand = Plus / Plus *F Query: 12 aaccagagaangggtctgacnctcccaatcccatgggtttctctccgcgcatccacgacc 71 *F |||||||||| ||||||||| ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1105 aaccagagaaggggtctgacgctcccaatcccatgggtttctctccgcgcatccacgacc 1164 *F Query: 72 gcagccnaccgcccgcgtttccgcgtaccattaagatcagggatcgtccatccngttatc 131 *F |||||| |||||||||||||||||||| ||||||||||||||||||||||||| |||||| *F Sbjct: 1165 gcagccaaccgcccgcgtttccgcgtagcattaagatcagggatcgtccatccagttatc 1224 *F Query: 132 natttctagaggaaatgatttcgngcttcaaatacncctgcaaagtcnccnnntacaagg 191 *F ||||||||||||||||||||| ||||||||||| ||||||||||| || ||||||| *F Sbjct: 1225 agtttctagaggaaatgatttcgcgcttcaaatacgcctgcaaagtcaccaagtacaagg 1284 *F Query: 192 attactantcggcgctgaacttctttatcgantacagcaaaaagtngggccactgcatcc 251 *F ||||||| ||||||||||||||||||||||| ||||||||||||| |||||| ||||||| *F Sbjct: 1285 attactattcggcgctgaacttctttatcgagtacagcaaaaagtcgggccagtgcatcc 1344 *F Query: 252 tgtccagaagcgtgctgcaaaccctgttcagcgcccacatgngttatggcgcacngtaaa 311 *F ||||||||||||||||||||||||||||||||||| ||||| | |||||||||| | ||| *F Sbjct: 1345 tgtccagaagcgtgctgcaaaccctgttcagcgccaacatgcg-tatggcgcacgg-aaa 1402 *F Query: 312 gcttcccatnaaagcanttccngcgcca-tcggttcaggtcttcaactcnccacccgtat 370 *F ||||||||| ||||| |||| |||||| |||||||||||||||||||| |||||||||| *F Sbjct: 1403 gcttcccatg-aagcagttcctgcgccactcggttcaggtcttcaactcgccacccgtat 1461 *F Query: 371 tgaatgccaancacccngtggctgcccatcccaacgtgc 409 *F |||||||||| || || ||||||||| ||||||| |||| *F Sbjct: 1462 tgaatgccaagcatccggtggctgccgatcccaaggtgc 1500 *F \# *F anon-EST:ParkEST125 = CG4182 *F >anon-EST:ParkEST125 *F gaattctgac caactgaccg gaggtccaca aaaaaaatag cgagcaatcc aaaaaaatcg *F aacaattaaa acccatagct ccaaaatgaa cccactagga atgttgagcc tgggcttaat *F ggtgtgcctt atcggcgcct tcagccggat tgcatcggcc aagctggagg agaagttctc *F gtggaagcaa ctggccttcg attggcccac tccggaggcc gaggcggagg ccaagtcgaa *F tggccactac atcgtggaga acaacctgcc gctggtcgtg gagcgttggc aaaacagaat *F ctttgtcact gtgccaagat ggaaggctgg tgttgctgcc acactcaact acatcgacat *F caattcgacg gagaagtcgc caaagctgca tccgtatccc agttgggagg ccaacaagtt *F gccgcgcggc cgc *F Database: dmel_all_transcript_r320 *F >CG4182-c-RA type=mRNA; *F loc=2L:join(15013556..15013881,15014671..15015076, *F 15015136..15015927); ID=yellow-c-RA; name=yellow-c-RA; *F db_xref='CG4182,FlyBase:FBgn0041713'; len=1524 *F Length = 1524 *F Genome Map *F Score = 644 bits (335), Expect = 0.0 *F Identities = 341/344 (99%) *F Strand = Plus / Plus *F Query: 80 tccaaaatgaacccactaggaatgttgagcctgggcttaatggtgtgccttatcggcgcc 139 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 88 tccaaaatgaacccactaggaatgttgagcctgggcttaatggtgtgccttatcggcgcc 147 *F Query: 140 ttcagccggattgcatcggccaagctggaggagaagttctcgtggaagcaactggccttc 199 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148 ttcagccggattgcatcggccaagctggaggagaagttctcgtggaagcaactggccttc 207 *F Query: 200 gattggcccactccggaggccgaggcggaggccaagtcgaatggccactacatcgtggag 259 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208 gattggcccactccggaggccgaggcggaggccaagtcgaatggccactacatcgtggag 267 *F Query: 260 aacaacctgccgctggtcgtggagcgttggcaaaacagaatctttgtcactgtgccaaga 319 *F |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 268 aacaacctgccgctgggcgtggagcgttggcaaaacagaatctttgtcactgtgccaaga 327 *F Query: 320 tggaaggctggtgttgctgccacactcaactacatcgacatcaattcgacggagaagtcg 379 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 328 tggaaggctggtgttgctgccacactcaactacatcgacattaattcgacggagaagtcg 387 *F Query: 380 ccaaagctgcatccgtatcccagttgggaggccaacaagttgcc 423 *F ||||||||||||||||||||||||||||||||||||||| |||| *F Sbjct: 388 ccaaagctgcatccgtatcccagttgggaggccaacaagctgcc 431 *F \# *F anon-EST:ParkEST132 = CG10992 *F >anon-EST:ParkEST132 *F gaattctgac taactgacaa cgtcagtcgc gaatcggaat gaaaataaaa ggaggggagt *F acgaaaacaa gaacggagca gagagcccgc ttaatatgac gcgtcatttt ccaccatttt *F actgctgtga tttgtttgaa ttttgctgtg tcagcgcact ggaaattcgt tggacaagaa *F taatgaaact actgctcctg gtggccaccg cggcctccgt ggcggcactc acgtccggtg *F agccgtcact gctctcggat gagttcatcg aggtggtgcg cagtaaggcc aaaacctgga *F cggtgggtcg caatttcgat gcatccgtaa cggagccgcg cggccgc *F Database: dmel_all_transcript_r320 *F >CG10992-RA type=mRNA; *F loc=X:complement(13510677..13511309,13511818..13511985, *F 13512132..13512392,13512469..13512840); ID=CG10992-RA; *F name=CG10992-RA; db_xref='CG10992,FlyBase:FBgn0030521'; *F len=1434 *F Length = 1434 *F Genome Map *F Score = 514 bits (267), Expect = e-145 *F Identities = 277/282 (98%) *F Strand = Plus / Plus *F Query: 54 ggggagtacgaaaacaagaacggagcagagagcccgcttaatatgacgcgtcattttcca 113 *F ||| ||||||||||||||||||||||||||||||||||||||| |||||||||||||||| *F Sbjct: 124 gggcagtacgaaaacaagaacggagcagagagcccgcttaatacgacgcgtcattttcca 183 *F Query: 114 ccattttactgctgtgatttgtttgaattttgctgtgtcagcgcactggaaattcgttgg 173 *F ||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| *F Sbjct: 184 ccattttactgctgtgatttgtttgaattttgctgtgtcagcgcattggaaattcgttgg 243 *F Query: 174 acaagaataatgaaactactgctcctggtggccaccgcggcctccgtggcggcactcacg 233 *F |||||||||||||| ||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 244 acaagaataatgaatctattgctcctggtggccaccgcggcctccgtggcggcactcacg 303 *F Query: 234 tccggtgagccgtcactgctctcggatgagttcatcgaggtggtgcgcagtaaggccaaa 293 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 304 tccggtgagccgtcactgctctcggatgagttcatcgaggtggtgcgcagtaaggccaaa 363 *F Query: 294 acctggacggtgggtcgcaatttcgatgcatccgtaacggag 335 *F |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 364 acctggacggtgggtcgcaatttcgatgcatccgtaacggag 405 *F Score = 68.0 bits (35), Expect = 5e-11 *F Identities = 37/38 (97%) *F Strand = Plus / Minus *F Query: 19 aacgtcagtcgcgaatcggaatgaaaataaaaggaggg 56 *F ||||||||| |||||||||||||||||||||||||||| *F Sbjct: 1371 aacgtcagttgcgaatcggaatgaaaataaaaggaggg 1334 *F \# *F anon-EST:ParkEST325 = CG6199 *F >anon-EST:ParkEST325 *F gaattctgac taactgacgg ggggggggtc gcagagaagg ctgttcatcc ggccgataac *F gcaccatgaa gttcatcagc gcgcgtggag gattgtggaa gtaacccgta aaggcgcgct *F cttgcgggtg ggactcccga tattttgtgg agcggaaggt gttttgattt ccttagcatt *F tagcacaaaa aaatgcaact agtataaagt actgttcact ataacaattt ttaaccaccg *F atagcgagtg cttcactgtg tgtgcgagta agagagagag agagcaacta gctccagcat *F catgagaata caacaaagcg ccttgttgtt gttgctgctg gccgtgacgt cgcaaggaga *F tgccgagtcc ccgcgcggcc gc *F Database: dmel_all_transcript_r320 *F >CG6199-RA type=mRNA; *F loc=3L:complement(11155698..11156242,11156302..11156736, *F 11156798..11156924,11159747..11159872, *F 11159932..11160033,11160154..11160407, *F 11160475..11161006,11161065..11161204, *F 11162437..11162522,11163266..11163578); ID=CG6199-RA; *F name=CG6199-RA; db_xref='CG6199,FlyBase:FBgn0036147'; *F len=2660 *F Length = 2660 *F Genome Map *F Score = 402 bits (209), Expect = e-111 *F Identities = 230/251 (91%) *F Strand = Plus / Plus *F Query: 120 tcttgcgggtgggactcccgatattttgtggagcggaaggtgttttgatttccttagcat 179 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 53 tcttgcgggtgggactcccgatattttgtggagcggaaggtgttttgatttccttagcat 112 *F Query: 180 ttagcacnnnnnnntgcaactagtataaagtactgttcactataacaatttttaaccacc 239 *F ||||||| |||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 113 ttagcacaaaaaaatgcaactagtataaagtactgttcactataacaatttttaaccacc 172 *F Query: 240 gatagcgagtgcttcactgtgtgtgcgagtannnnnnnnnnnnnncaactagctccagca 299 *F ||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 173 gatagcgagtgcttcactgtgtgtgcgagtaagagagagagagagcaactagctccagca 232 *F Query: 300 tcatgagaatacaacaaagcgccttgttgttgttgctgctggccgtgacgtcgcaaggag 359 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 233 tcatgagaatacaacaaagcgccttgttgttgttgctgctggccgtgacgtcgcaaggag 292 *F Query: 360 atgccgagtcc 370 *F ||||||||||| *F Sbjct: 293 atgccgagtcc 303 *F Score = 181 bits (94), Expect = 4e-45 *F Identities = 96/97 (98%) *F Strand = Plus / Minus *F Query: 29 tcgcagagaaggctgttcatccggccgataacgcaccatgaagttcatcagcgcgcgtgg 88 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2178 tcgcagagaaggctgttcatccggccgataacgcaccatgaagttcatcagcgcgcgtgg 2119 *F Query: 89 aggattgtggaagtaacccgtaaaggcgcgctcttgc 125 *F ||||||||||||||||||||||||||||||||| ||| *F Sbjct: 2118 aggattgtggaagtaacccgtaaaggcgcgctcctgc 2082 *F \# *F \------ *F Date: Thu, 1 Jul 2004 09:56:05 \+0100 (BST) *F From: 'Michael Ashburner (Genetics)' *F Subject: Death to anon-ESTs 3 *F To: crosby@morgan.harvard.edu, ma11@gen.cam.ac.uk, gm119@gen.cam.ac.uk *F Cc: gm119@gen.cam.ac.uk *F Here is the last bunch of anon-ESTs, algorithm as before. enjoy *F M *F anon-EST:Posey1 = new ? *F anon-EST:Posey101 = CG32172 *F anon-EST:Posey102 = CG3056 *F anon-EST:Posey107 = new ? *F anon-EST:Posey11 = CG8086? *F anon-EST:Posey117 = CG3257 *F anon-EST:Posey119 = ? end of MTA-like ? *F anon-EST:Posey126 = ? end of Df31 ? *F anon-EST:Posey139 = end of BEST:GH08269 ? *F anon-EST:Posey146 = new? *F anon-EST:Posey152 = ? *F anon-EST:Posey155 = ? *F anon-EST:Posey156 = EST GM16138 *F anon-EST:Posey171 = CG12187 *F anon-EST:Posey175 = end of CG13203 ? *F anon-EST:Posey178 = ? end CG31369 ? *F anon-EST:Posey186 = CG6549 *F anon-EST:Posey204 = CG10071 *F anon-EST:Posey215 = CG2985? *F anon-EST:Posey216 = end of CG32423 *F anon-EST:Posey221 = CG3620 *F anon-EST:Posey226 = end of CG32712 ? *F anon-EST:Posey232 = CG18815 *F anon-EST:Posey243 = CG32172 *F anon-EST:Posey246 = CG5627 *F anon-EST:Posey247 = CG5119 *F anon-EST:Posey249 = CG5119 *F anon-EST:Posey25 = CG30185 *F anon-EST:Posey251 = ? *F anon-EST:Posey259 =CG8177 *F anon-EST:Posey262 = CG15209 *F anon-EST:Posey263 = ? end of l(3)82Fd ? *F anon-EST:Posey264 = not in genome, and a pretty odd seq *F anon-EST:Posey268 = ? *F anon-EST:Posey277 = CG31451 *F anon-EST:Posey280 = ? *F anon-EST:Posey285 = CG6869 *F anon-EST:Posey288 = CG13993 *F anon-EST:Posey289 = end of lace ? *F anon-EST:Posey292 = CG32172 *F anon-EST:Posey294 = CG2848 *F anon-EST:Posey35 = CG32172 *F anon-EST:Posey37 = CG9282 *F anon-EST:Posey5 = CG1263 *F anon-EST:Posey52 = CG31158? *F anon-EST:Posey55 = CG11739 *F anon-EST:Posey59 = CG8472 *F anon-EST:Posey6 = CG6253 *F anon-EST:Posey64 = CG11593 *F anon-EST:Posey65 = CG16982 *F anon-EST:Posey67 = CG8309 *F anon-EST:Posey69 = CG31543 *F anon-EST:Posey7 = end of CHES-1-like ? *F anon-EST:Posey79 = CG5889 *F anon-EST:Posey83 = ? *F anon-EST:Posey9 = CG9325 *F anon-EST:Posey93 = end of dlg1 ? *F anon-EST:PoseyA1 = qbert *F anon-EST:CL1d7 = CG10391 *F anon-EST:CLB3 = CG6736 *F \# *F anon-EST:Posey1 = new ? *F >anon-EST:Posey1 *F ggcacgagcc aaagttcact tgtctcagat tttaacttaa tttggagctt tatacagtat *F acagaagatt ttatttcacc acctatcata attcaaatta aaattgtttt tgaaatagta *F aagcggtaaa ttaaaaacta tatgtctatg aaaatctgtc tataaaaata tgtataattt *F gattaaatta atttttcatt tgtaagaatt actataatta tttaaaattt aaaaactctt *F gtacggcttt ttgactcaaa atctttgctt acaaaaaaga tttgtccccg cataagtcaa *F cacggtgttg acatggtgtt ctcatggtac tcaaaaataa ttaagtatgt atcaaagtat *F tagtgcgaaa aaatgtgaaa tagaaagtta acattttagc atataggaat acaactatta *F aaatgaattt aaatgtttga atttgttaca aaataaagtt aatttataaa cagcaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE002665|gb|AE002665|arm:3L 23206183..23352213 *F estimated-cyto:80E3-80F6 gadfly-seqname:AE002665 *F seq_release:3 *F Length = 146031 *F Score = 896 bits (466), Expect = 0.0 Genome Map *F Identities = 468/469 (99%) *F Strand = Plus / Minus *F Query: 9 ccaaagttcacttgtctcagattttaacttaatttggagctttatacagtatacagaaga 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 24285 ccaaagttcacttgtctcagattttaacttaatttggagctttatacagtatacagaaga 24226 *F Query: 69 ttttatttcaccacctatcataattcaaattaaaattgtttttgaaatagtaaagcggta 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 24225 ttttatttcaccacctatcataattcaaattaaaattgtttttgaaatagtaaagcggta 24166 *F Query: 129 aattaaaaactatatgtctatgaaaatctgtctataaaaatatgtataatttgattaaat 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 24165 aattaaaaactatatgtctatgaaaatctgtctataaaaatatgtataatttgattaaat 24106 *F Query: 189 taatttttcatttgtaagaattactataattatttaaaatttaaaaactcttgtacggct 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 24105 taatttttcatttgtaagaattactataattatttaaaatttaaaaactcttgtacggct 24046 *F Query: 249 ttttgactcaaaatctttgcttacaaaaaagatttgtccccgcataagtcaacacggtgt 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 24045 ttttgactcaaaatctttgcttacaaaaaagatttgtccccgcataagtcaacacggtgt 23986 *F Query: 309 tgacatggtgttctcatggtactcaaaaataattaagtatgtatcaaagtattagtgcga 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 23985 tgacatggtgttctcatggtactcaaaaataattaagtatgtatcaaagtattagtgcga 23926 *F Query: 369 aaaaatgtgaaatagaaagttaacattttagcatataggaatacaactattaaaatgaat 428 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 23925 aaaaatgtgaaatagaaagttaacattttagcatataggaatacaactattaaaatgaat 23866 *F Query: 429 ttaaatgtttgaatttgttacaaaataaagttaatttataaacagcaaa 477 *F |||||||| |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 23865 ttaaatgtatgaatttgttacaaaataaagttaatttataaacagcaaa 23817 *F >gadfly|SEG:AE003507|gb|AE003507|arm:X 17583607..17883410 *F estimated-cyto:16E1-16F7 gadfly-seqname:AE003507 *F seq_release:3 *F Length = 299804 *F Score = 43.0 bits (22), Expect = 0.007 Genome Map *F Identities = 24/25 (96%) *F Strand = Plus / Plus *F Query: 170 atgtataatttgattaaattaattt 194 *F ||||||||||| ||||||||||||| *F Sbjct: 87125 atgtataatttaattaaattaattt 87149 *F \# *F anon-EST:Posey101 = CG32172 *F >anon-EST:Posey101 *F ggcacgagcc gcagcaacga cggttgggca catcccgtct catccccact ctctcgaatt *F gtgtccgttg gtagccgaag ttacgattgc aaaactttcg tcatctctct ctcactctct *F gaagcaaaag ccaaggccgg agcaggtgca tatggtgcca acccccctgg cccccccaaa *F aggggggtgg tgctggtgca attgcaaagg cacaggagca caggagcagc taaataaaca *F agaaagcaaa atcaacaacc gcaaaagtct aagacatgta tcacgaattg aaaatgataa *F tgacaaaaaa aaa *F Database: dmel_all_transcript_r320 *F >CG32172-RA type=mRNA; loc=3L:complement(17348426..17350337); Genome Map *F ID=noe-RA; name=noe-RA; *F db_xref='CG32172,FlyBase:FBgn0026197'; len=1912 *F Length = 1912 *F Score = 442 bits (230), Expect = e-124 *F Identities = 263/296 (88%) *F Strand = Plus / Plus *F Query: 9 ccgcagcaacgacggttgggcacatcccgtctcatccccactctctcgaattgtgtccgt 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 126 ccgcagcaacgacggttgggcacatcccgtctcatccccactctctcgaattgtgtccgt 185 *F Query: 69 tggtagccgaagttacgattgcaaaactttcgtcannnnnnnnnnnnnnnnngaagcaaa 128 *F ||||||||||||||||||||||||||||||||||| |||||||| *F Sbjct: 186 tggtagccgaagttacgattgcaaaactttcgtcatctctctctcactctctgaagcaaa 245 *F Query: 129 agccaaggccggagcaggtgcatatggtgccaannnnnnnnnnnnnnnnaaaaggggggt 188 *F ||||||||||||||||||||||||||||||||| ||||||||||| *F Sbjct: 246 agccaaggccggagcaggtgcatatggtgccaacccccctggcccccccaaaaggggggt 305 *F Query: 189 ggtgctggtgcaattgcaaaggcacaggagcacaggagcagctaaataaacaagaaagca 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 306 ggtgctggtgcaattgcaaaggcacaggagcacaggagcagctaaataaacaagaaagca 365 *F Query: 249 aaatcaacaaccgcaaaagtctaagacatgtatcacgaattgaaaatgataatgac 304 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 366 aaatcaacaaccgcaaaagtctaagacatgtatcacgaattgaaaatgataatgac 421 *F \# *F anon-EST:Posey102 = CG3056 *F >anon-EST:Posey102 *F ggcacgagat aaacttgaaa ttttccaacc agcatacccg tgagacccag aggtccgaaa *F tttttttttt tagtattgat aatctctaaa cgtaaatcct agtctcgtag caatatcata *F gttaaggtga cttttttttc agaataaact taaagcacga aagaaataca tgtatttaaa *F ccttaaatcc taaagtttat aataaacgaa cacaaattga attcttgagt ctgcgagcga *F gatggtgcga tgcagcctca gcgaaagaga gcgagagaga tgatacagat ttgaggtaca *F gtcagcacag tacagtgcgg atcagagctt aagttgacga tatactattg ttgtaaattg *F atgttaggct aagcaaataa atagttaaaa tgttcaaaaa *F Database: dmel_all_transcript_r320 *F >CG3056-RA type=mRNA; *F loc=X:join(1128003..1128100,1128668..1128807, *F 1128888..1129211,1129352..1129459,1129523..1129704, *F 1129898..1130484,1130595..1132177); ID=CG3056-RA; *F name=CG3056-RA; db_xref='CG3056,FlyBase:FBgn0024987'; *F len=3022 *F Length = 3022 *F Genome Map *F Score = 319 bits (166), Expect = 8e-87 *F Identities = 192/212 (90%), Gaps = 1/212 (0%) *F Strand = Plus / Plus *F Query: 9 ataaacttgaaattttccaaccagcatacccgtgagacccagaggtccgaaannnnnnnn 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2811 ataaacttgaaattttccaaccagcatacccgtgagacccagaggtccgaaatttttttt 2870 *F Query: 69 nnn-agtattgataatctctaaacgtaaatcctagtctcgtagcaatatcatagttaagg 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2871 ttttagtattgataatctctaaacgtaaatcctagtctcgtagcaatatcatagttaagg 2930 *F Query: 128 tgacnnnnnnnncagaataaacttaaagcacgaaagaaatacatgtatttaaaccttaaa 187 *F |||| |||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2931 tgacttttttttcagaataaacttaaagcacgaaagaaatacatgtatttaaaccttaaa 2990 *F Query: 188 tcctaaagtttataataaacgaacacaaattg 219 *F |||||||||||||||||||||||||||||||| *F Sbjct: 2991 tcctaaagtttataataaacgaacacaaattg 3022 *F >CG3056-RB type=mRNA; *F loc=X:join(1128003..1128100,1128668..1128807, *F 1128888..1129211,1129352..1129459,1129523..1129704, *F 1129898..1132120); ID=CG3056-RB; name=CG3056-RB; *F db_xref='CG3056,FlyBase:FBgn0024987'; len=3075 *F Length = 3075 *F Genome Map *F Score = 210 bits (109), Expect = 8e-54 *F Identities = 135/155 (87%), Gaps = 1/155 (0%) *F Strand = Plus / Plus *F Query: 9 ataaacttgaaattttccaaccagcatacccgtgagacccagaggtccgaaannnnnnnn 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2921 ataaacttgaaattttccaaccagcatacccgtgagacccagaggtccgaaatttttttt 2980 *F Query: 69 nnn-agtattgataatctctaaacgtaaatcctagtctcgtagcaatatcatagttaagg 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2981 ttttagtattgataatctctaaacgtaaatcctagtctcgtagcaatatcatagttaagg 3040 *F Query: 128 tgacnnnnnnnncagaataaacttaaagcacgaaa 162 *F |||| ||||||||||||||||||||||| *F Sbjct: 3041 tgacttttttttcagaataaacttaaagcacgaaa 3075 *F \# *F anon-EST:Posey107 = new ? *F >anon-EST:Posey107 *F ggcacgagaa agatccatgt tgcatcctcg gcagttgtgt gttgtttatg ccgagaattg *F gagcgcatcg acaagttaca gcaaatgttg catcatattg ttgccctgct gcctgttgtg *F ctggcaaatg aagtggaaat tgagtctgct cttgttgact gcggttgcat ttcctttttt *F tttttttttt *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003636|gb|AE003636|arm:2L 12386446..12632675 *F estimated-cyto:33E3-33F2 gadfly-seqname:AE003636 *F seq_release:3 *F Length = 246230 *F Score = 302 bits (157), Expect = 2e-81 Genome Map *F Identities = 159/160 (99%) *F Strand = Plus / Minus *F Query: 14 tccatgttgcatcctcggcagttgtgtgttgtttatgccgagaattggagcgcatcgaca 73 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 120382 tccatgttgcatcctcggcagttgtgtgttgtttatgccgagaattggagcgcatcgaca *F 120323 *F Query: 74 agttacagcaaatgttgcatcatattgttgccctgctgcctgttgtgctggcaaatgaag 133 *F |||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 120322 agttgcagcaaatgttgcatcatattgttgccctgctgcctgttgtgctggcaaatgaag *F 120263 *F Query: 134 tggaaattgagtctgctcttgttgactgcggttgcatttc 173 *F |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 120262 tggaaattgagtctgctcttgttgactgcggttgcatttc 120223 *F \# *F anon-EST:Posey11 = CG8086? *F >anon-EST:Posey11 *F ggcacgaggg tatctgtatc tctgacatga tcaacaatca tctcaactca gtgaaaacgt *F ttttatgttc tttcgacttc atttcacgct ctccaaaact gcgaattaaa tatgagctca *F ttcccaaaat ttagacaatt tccaggcggc caacttgggg gcttttacgc ccaaatacgg *F cggccgacgt ttcctttagc ccacattgag ttgtccgaaa aagccagttc ggaattgggt *F ttccagacgg cagatcgacc cacgcagaaa ctccatgact ccgcgatata tagacataca *F gccaaatagc cataattcgt gtgctgtctc tattaactat tgcctctttt tttttgtaaa *F agtttaatta acgccgaaca agttgagcaa ttcctttcca aaaacagaaa acgacaacaa *F gtgagtgagc cagccggcaa aaggccaaag gccgaaaaga gctggtaaat gaaaacatga *F gagccaagaa gcaaaactga actctcgtga ttgtgataaa aacgccaaga ccgactgacg *F tcagctgtgt gaactagttt gcgatcgtcc aaatctaatt aagtttctca acattctcgg *F tgctctgcag cgcgaacgtg ttttggcttt gaaaaccaag agaaagctgg gaaatgaggc *F gggaaaaatt ggcaaaccct cactgaaact tggttctcaa ggcacaaggc caatgttttc *F aaatagctgt tgataattac taaatttaat gggggaccat cacacatccg tttccaaatt *F ctagagcctg agatcactgg ctgctgctga cacaatttgc atgtttggat acataaatat *F ccgtttgggt cattttataa atatttattt agcgattatt gcatttcccc ttagcagctt *F atttttctat ttaacctttt accattcgtt actttgttat tttgtatctt tttttttttt *F tttgtttttg gccaacgcgc ttttgtgttg tgcctgcgcc ataatcagtg actttgaaaa *F atgagctgct gactcgggtc gaccggttat tagagctgat gtcatgggct tcgtgggaca *F agtgaagtaa aggtatcaag aaccgcggaa gggttacatc tatgccaaca agtgacatca *F atacgggaac gcgattgtca ttgactaaac aatgggagga gctgggtgga tttatttggg *F ggcggagcag aacagctgac tggcggtggg gaaacctagc cctatgccac caggaaatgc *F caatagttag acagacagac agtcaatgaa ctttggggtc gggtcagcac gcaatcccac *F tggctgctcc ggtcgggctc cacctgactt ggttaacgcc tactccagtt tatccttacc *F gcaggctact ttacgatttc gggtatctcg tcctgtttcc gttgtatcgt gtgatttccg *F taactttctt ttacacagta aaaaacttca ctttattgcg gtgctaatag cgcgtagtgc *F atacagtcct tgccaggatt agtcgcgatt tttgggcgat attcaaaaca tctgttcacc *F cgaaagagat tgctatattc gttgttattg tagttgggtt tcgtggctga tttattattt *F cgttgattcg ttcgttgatt gggttcaagt gacagccgct ttcgagtgtc ggtctgccga *F ctggcgtttg aagtctacta gaggttttca aaacaaaaca cactttgctg atttatttat *F accgattgga ttggccgcta tgagctgagt ttgtttcagt gaaagagaag agagctagct *F agaaccgca *F Database: dmel_all_transcript_r320 *F >CG8086-RA type=mRNA; *F loc=2L:complement(8236776..8236963,8237201..8237454, *F 8244459..8244764,8244826..8245006,8249309..8249733); *F ID=CG8086-RA; name=CG8086-RA; *F db_xref='CG8086,FlyBase:FBgn0032010'; len=1354 *F Length = 1354 *F Genome Map *F Score = 821 bits (427), Expect = 0.0 *F Identities = 427/427 (100%) *F Strand = Plus / Minus *F Query: 1378 accgcaggctactttacgatttcgggtatctcgtcctgtttccgttgtatcgtgtgattt 1437 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 427 accgcaggctactttacgatttcgggtatctcgtcctgtttccgttgtatcgtgtgattt 368 *F Query: 1438 ccgtaactttcttttacacagtaaaaaacttcactttattgcggtgctaatagcgcgtag 1497 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 367 ccgtaactttcttttacacagtaaaaaacttcactttattgcggtgctaatagcgcgtag 308 *F Query: 1498 tgcatacagtccttgccaggattagtcgcgatttttgggcgatattcaaaacatctgttc 1557 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 307 tgcatacagtccttgccaggattagtcgcgatttttgggcgatattcaaaacatctgttc 248 *F Query: 1558 acccgaaagagattgctatattcgttgttattgtagttgggtttcgtggctgatttatta 1617 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 247 acccgaaagagattgctatattcgttgttattgtagttgggtttcgtggctgatttatta 188 *F Query: 1618 tttcgttgattcgttcgttgattgggttcaagtgacagccgctttcgagtgtcggtctgc 1677 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 187 tttcgttgattcgttcgttgattgggttcaagtgacagccgctttcgagtgtcggtctgc 128 *F Query: 1678 cgactggcgtttgaagtctactagaggttttcaaaacaaaacacactttgctgatttatt 1737 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 127 cgactggcgtttgaagtctactagaggttttcaaaacaaaacacactttgctgatttatt 68 *F Query: 1738 tataccgattggattggccgctatgagctgagtttgtttcagtgaaagagaagagagcta 1797 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 67 tataccgattggattggccgctatgagctgagtttgtttcagtgaaagagaagagagcta 8 *F Query: 1798 gctagaa 1804 *F ||||||| *F Sbjct: 7 gctagaa 1 *F \# *F anon-EST:Posey117 = CG3257 *F >anon-EST:Posey117 *F ggcacgagcg gcacttttgt gccattttac acccaaatcg atggtgtttt ttttggggaa *F aacttaaaac caagttagct ccatcgaaat ggcgatacga acctagttac tcgaacaaac *F gaatatgcaa acttagtctg aaggtcaatt ggtcctcatt tagagtcgtt gaatgttcat *F tacttgcttt ccgtgtgcgt tgacgacatt taccgaagat ggatctggtt tcactggaca *F aatccactcc gattagcact cgaaaatcga aagatagtga agcaaaaccg actaagtctg *F ttagctgtta gatggaagta ggaaattaca aataaaacgc gtaggaaaag cgaatcgata *F ccaagtactt tttccaagta gctaagtagc tgagtaagag aggagcccga actcgcatca *F gcatagaaat aattcgaaag ggatcgtagg aagtgtaagg attgtatttc gggctctggg *F aggttttcaa agtcggactc ctgtgcttga aaaacatata taaatgtacg aatatactaa *F acacctcaaa aaa *F Database: dmel_all_transcript_r320 *F >CG3257-RA type=mRNA; *F loc=2R:join(19160357..19160540,19163153..19163174, *F 19163464..19163822,19164280..19164389,19164460..19165042, *F 19165101..19165402,19165478..19166838); ID=CG3257-RA; *F name=CG3257-RA; db_xref='CG3257,FlyBase:FBgn0034978'; *F len=2921 *F Length = 2921 *F Genome Map *F Score = 983 bits (511), Expect = 0.0 *F Identities = 530/541 (97%), Gaps = 1/541 (0%) *F Strand = Plus / Plus *F Query: 9 cggcacttttgtgccattttacacccaaatcgatggtgnnnnnnnn-ggggaaaacttaa 67 *F |||||||||||||||||||||||||||||||||||||| ||||||||||||| *F Sbjct: 2375 cggcacttttgtgccattttacacccaaatcgatggtgtttttttttggggaaaacttaa 2434 *F Query: 68 aaccaagttagctccatcgaaatggcgatacgaacctagttactcgaacaaacgaatatg 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2435 aaccaagttagctccatcgaaatggcgatacgaacctagttactcgaacaaacgaatatg 2494 *F Query: 128 caaacttagtctgaaggtcaattggtcctcatttagagtcgttgaatgttcattacttgc 187 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2495 caaacttagtctgaaggtcaattggtcctcatttagagtcgttgaatgttcattacttgc 2554 *F Query: 188 tttccgtgtgcgttgacgacatttaccgaagatggatctggtttcactggacaaatccac 247 *F ||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2555 tttccgtgtacgttgacgacatttaccgaagatggatctggtttcactggacaaatccac 2614 *F Query: 248 tccgattagcactcgaaaatcgaaagatagtgaagcaaaaccgactaagtctgttagctg 307 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2615 tccgattagcactcgaaaatcgaaagatagtgaagcaaaaccgactaagtctgttagctg 2674 *F Query: 308 ttagatggaagtaggaaattacaaataaaacgcgtaggaaaagcgaatcgataccaagta 367 *F |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 2675 ttagatggaagtaggaaattacaagtaaaacgcgtaggaaaagcgaatcgataccaagta 2734 *F Query: 368 ctttttccaagtagctaagtagctgagtaagagaggagcccgaactcgcatcagcataga 427 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2735 ctttttccaagtagctaagtagctgagtaagagaggagcccgaactcgcatcagcataga 2794 *F Query: 428 aataattcgaaagggatcgtaggaagtgtaaggattgtatttcgggctctgggaggtttt 487 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2795 aataattcgaaagggatcgtaggaagtgtaaggattgtatttcgggctctgggaggtttt 2854 *F Query: 488 caaagtcggactcctgtgcttgaaaaacatatataaatgtacgaatatactaaacacctc 547 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2855 caaagtcggactcctgtgcttgaaaaacatatataaatgtacgaatatactaaacacctc 2914 *F Query: 548 a 548 *F | *F Sbjct: 2915 a 2915 *F \# *F anon-EST:Posey119 = ? end of MTA-like ? *F >anon-EST:Posey119 *F ggcacgagaa acaaactaac agagagacaa gcaacagaag caaatcaaac agagctatat *F acataattat atacctatat atatatatta tatacagata tacagtataa cagtacatac *F atggcgcatt ggtcaagaac agtacgcata atccttttta cagagcaaga aactgacgaa *F gacgacgacg tgacggccag acaagcaacc gaaaaagttt tccaaataaa attttaaaac *F taaacatttt tcctaaaaa *F Database: dmel_all_scaffolds_r310 *F gadfly|SEG:AE003602|gb|AE003602|arm:3R 1266332..1562422 *F estimated-cyto:83A3-83C1 gadfly-seqname:AE003602 *F seq_release:3 *F Length = 296091 *F Score = 289 bits (150), Expect = 3e-77 Genome Map *F Identities = 150/150 (100%) *F Strand = Plus / Minus *F Query: 110 acagtacatacatggcgcattggtcaagaacagtacgcataatcctttttacagagcaag 169 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 198187 acagtacatacatggcgcattggtcaagaacagtacgcataatcctttttacagagcaag *F 198128 *F Query: 170 aaactgacgaagacgacgacgtgacggccagacaagcaaccgaaaaagttttccaaataa 229 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 198127 aaactgacgaagacgacgacgtgacggccagacaagcaaccgaaaaagttttccaaataa *F 198068 *F Query: 230 aattttaaaactaaacatttttcctaaaaa 259 *F |||||||||||||||||||||||||||||| *F Sbjct: 198067 aattttaaaactaaacatttttcctaaaaa 198038 *F Score = 91.1 bits (47), Expect = 1e-17 Genome Map *F Identities = 47/47 (100%) *F Strand = Plus / Minus *F Query: 9 aaacaaactaacagagagacaagcaacagaagcaaatcaaacagagc 55 *F ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 198288 aaacaaactaacagagagacaagcaacagaagcaaatcaaacagagc 198242 *F Score = 37.2 bits (19), Expect = 0.19 Genome Map *F Identities = 27/31 (87%) *F Strand = Plus / Minus *F Query: 218 ttttccaaataaaattttaaaactaaacatt 248 *F ||||| ||||| |||| ||||||||||||| *F Sbjct: 219884 ttttcttaataatatttaaaaactaaacatt 219854 *F \# *F anon-EST:Posey126 = ? end of Df31 ? *F >anon-EST:Posey126 *F ggcacgaggt tttttttttt ttaaaatgct ttcgaaatcg tacctttttt atttgtaatc *F aaggtaccac ctatttcaca ttcccaatta cattttattt acttgtctaa gcctacgaaa *F tagtctgcga cgaaactctc attaatgttc aatgtttaac aacgatttaa tgggcaggaa *F aggtaatggc cagtaaatcc agcattcagc aaaatcaaga ttcgagatta ttaaaaaata *F aaatgaaacg taaaaattaa aatcaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003781|gb|AE003781|arm:2L 21394058..21699819 *F estimated-cyto:39E1-40B1 gadfly-seqname:AE003781 *F seq_release:3 *F Length = 305762 *F Score = 402 bits (209), Expect = e-111 Genome Map *F Identities = 209/209 (100%) *F Strand = Plus / Minus *F Query: 23 aaaatgctttcgaaatcgtaccttttttatttgtaatcaaggtaccacctatttcacatt 82 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65900 aaaatgctttcgaaatcgtaccttttttatttgtaatcaaggtaccacctatttcacatt 65841 *F Query: 83 cccaattacattttatttacttgtctaagcctacgaaatagtctgcgacgaaactctcat 142 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65840 cccaattacattttatttacttgtctaagcctacgaaatagtctgcgacgaaactctcat 65781 *F Query: 143 taatgttcaatgtttaacaacgatttaatgggcaggaaaggtaatggccagtaaatccag 202 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65780 taatgttcaatgtttaacaacgatttaatgggcaggaaaggtaatggccagtaaatccag 65721 *F Query: 203 cattcagcaaaatcaagattcgagattat 231 *F ||||||||||||||||||||||||||||| *F Sbjct: 65720 cattcagcaaaatcaagattcgagattat 65692 *F \# *F anon-EST:Posey139 = end of BEST:GH08269 ? *F >anon-EST:Posey139 *F ggcacgaggg agcaaggaga tttggcggag gagccgataa caacaataat gaaattaatg *F gagtaaacca attaaatcca tggaattccc aaatctaaaa taaaagctaa cttaatacgt *F ttttatttga atgaaagagt aacaaatcga ttgtaatatt gtatttcttg tgggcgtaaa *F acatcgacaa acgctcgacc cgttttacta gcatttaaaa caactattat ttaagagcat *F tttaaacatg aaaaaagaaa ataaaatcgg cagctacgcg cttctatcaa aagcacaatt *F gtaaaaagtc aatccagctc agctgcaatg aatgaatgaa gtctcccgaa tgaaaatgtt *F aaacgaaaaa acccgaacaa caaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003541|gb|AE003541|arm:3L 12268628..12534151 *F estimated-cyto:69B1-69C8 gadfly-seqname:AE003541 *F seq_release:3 *F Length = 265524 *F Score = 640 bits (333), Expect = 0.0 Genome Map *F Identities = 360/379 (94%), Gaps = 1/379 (0%) *F Strand = Plus / Plus *F Query: 9 ggagcaaggagatttggcggaggagccgataacaacaataatgaaattaatggagtaaac 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208657 ggagcaaggagatttggcggaggagccgataacaacaataatgaaattaatggagtaaac *F 208716 *F Query: 69 caattaaatccatggaattcccaaatctaaaataaaagctaacttaatacgtttttattt 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208717 caattaaatccatggaattcccaaatctaaaataaaagctaacttaatacgtttttattt *F 208776 *F Query: 129 gaatgaaagagtaacaaatcgattgtaatattgtatttcttgtgggcgtaaaacatcgac 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208777 gaatgaaagagtaacaaatcgattgtaatattgtatttcttgtgggcgtaaaacatcgac *F 208836 *F Query: 189 aaacgctcgacccgttttactagcatttaaaacaactattatttaagagcattttaaaca 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208837 aaacgctcgacccgttttactagcatttaaaacaactattatttaagagcattttaaaca *F 208896 *F Query: 249 tg-nnnnnnnnnnnnnnnntcggcagctacgcgcttctatcaaaagcacaattgtaaaaa 307 *F || ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208897 tgaaaaaaagaaaataaaatcggcagctacgcgcttctatcaaaagcacaattgtaaaaa *F 208956 *F Query: 308 gtcaatccagctcagctgcaatgaatgaatgaagtctcccgaatgaaaatgttaaacgaa 367 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 208957 gtcaatccagctcagctgcaatgaatgaatgaagtctcccgaatgaaaatgttaaacgaa *F 209016 *F Query: 368 aaaacccgaacaacaaaaa 386 *F ||||||| ||||| ||||| *F Sbjct: 209017 aaaaccccaacaaaaaaaa 209035 *F \# *F anon-EST:Posey146 = new? *F >anon-EST:Posey146 *F ggcacgaggg acattttaat gttaaaatca tggctatttt tccataaaca attacgccgt *F gattcggcgc tcgatatttt cactgagcgg tgattaattg atgaaaggac agtgtgtgga *F actgcagggt gtatataaaa ggaattataa atatgtgtat ttgtactcag cacttactga *F ggacaaagag atgctctgtt ggcactacaa tattaataag taacatattt gaaagttcgt *F ttgcgtgtta aaggctctag gtgcaaagcc ggacagcgat gaataaaaat aaatagtttc *F gttatatatc tgattgttta cttgacaaat aaagaataaa actgaaaaaa aaaaaaaaaa *F aa *F >gadfly|SEG:AABU01002756|gb|AABU01002756|arm:U 6723424..6923036 *F estimated-cyto:? gadfly-seqname:AABU01002756 seq_release:3 *F Length = 199613 *F Score = 341 bits (177), Expect = 1e-92 Genome Map *F Identities = 177/177 (100%) *F Strand = Plus / Plus *F Query: 168 cagcacttactgaggacaaagagatgctctgttggcactacaatattaataagtaacata 227 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 23411 cagcacttactgaggacaaagagatgctctgttggcactacaatattaataagtaacata 23470 *F Query: 228 tttgaaagttcgtttgcgtgttaaaggctctaggtgcaaagccggacagcgatgaataaa 287 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 23471 tttgaaagttcgtttgcgtgttaaaggctctaggtgcaaagccggacagcgatgaataaa 23530 *F Query: 288 aataaatagtttcgttatatatctgattgtttacttgacaaataaagaataaaactg 344 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 23531 aataaatagtttcgttatatatctgattgtttacttgacaaataaagaataaaactg 23587 *F Score = 312 bits (162), Expect = 5e-84 Genome Map *F Identities = 162/162 (100%) *F Strand = Plus / Plus *F Query: 9 ggacattttaatgttaaaatcatggctatttttccataaacaattacgccgtgattcggc 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 22647 ggacattttaatgttaaaatcatggctatttttccataaacaattacgccgtgattcggc 22706 *F Query: 69 gctcgatattttcactgagcggtgattaattgatgaaaggacagtgtgtggaactgcagg 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 22707 gctcgatattttcactgagcggtgattaattgatgaaaggacagtgtgtggaactgcagg 22766 *F Query: 129 gtgtatataaaaggaattataaatatgtgtatttgtactcag 170 *F |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 22767 gtgtatataaaaggaattataaatatgtgtatttgtactcag 22808 *F \# *F anon-EST:Posey152 = ? *F >anon-EST:Posey152 *F ggcacgaggt cgaaatatca ttttgaataa gttgctcaat ttgcttcgca aagtgattta *F ccgatttatc attctctatt ttcatatgtg tacaactaaa taaatgtatt gtaggcataa *F gctaaacaac acaataatgc aaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003795|gb|AE003795|arm:2R 14519511..14767606 *F estimated-cyto:56D9-56E5 gadfly-seqname:AE003795 *F seq_release:3 *F Length = 248096 *F Score = 264 bits (137), Expect = 5e-70 Genome Map *F Identities = 137/137 (100%) *F Strand = Plus / Minus *F Query: 9 gtcgaaatatcattttgaataagttgctcaatttgcttcgcaaagtgatttaccgattta 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65060 gtcgaaatatcattttgaataagttgctcaatttgcttcgcaaagtgatttaccgattta 65001 *F Query: 69 tcattctctattttcatatgtgtacaactaaataaatgtattgtaggcataagctaaaca 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65000 tcattctctattttcatatgtgtacaactaaataaatgtattgtaggcataagctaaaca 64941 *F Query: 129 acacaataatgcaaaaa 145 *F ||||||||||||||||| *F Sbjct: 64940 acacaataatgcaaaaa 64924 *F \# *F anon-EST:Posey155 = ? *F >anon-EST:Posey155 *F ggcacgagaa agatttaact gtggacgttg agttgaagtg ttgaagaaca cacaaaagaa *F aaaaacacga ggaaaacgta acgtctcttt ggtaagaaaa aggaaaacct aaagagcaaa *F tgctgtcatc caaaaaccaa aaaaaaaaaa cacaaaaaaa tgaaactgaa ctttttctga *F atcaataaaa gacaaattga tttcctgttg gaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003795|gb|AE003795|arm:2R 14519511..14767606 *F estimated-cyto:56D9-56E5 gadfly-seqname:AE003795 *F seq_release:3 *F Length = 248096 *F Score = 264 bits (137), Expect = 5e-70 Genome Map *F Identities = 137/137 (100%) *F Strand = Plus / Minus *F Query: 9 gtcgaaatatcattttgaataagttgctcaatttgcttcgcaaagtgatttaccgattta 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65060 gtcgaaatatcattttgaataagttgctcaatttgcttcgcaaagtgatttaccgattta 65001 *F Query: 69 tcattctctattttcatatgtgtacaactaaataaatgtattgtaggcataagctaaaca 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65000 tcattctctattttcatatgtgtacaactaaataaatgtattgtaggcataagctaaaca 64941 *F Query: 129 acacaataatgcaaaaa 145 *F ||||||||||||||||| *F Sbjct: 64940 acacaataatgcaaaaa 64924 *F \# *F anon-EST:Posey156 = EST GM16138 *F >anon-EST:Posey156 *F ggcacgagtc tgtttggggt attgtccaat tggttttaat gggtctattc ttctacataa *F atagtgtagc cctcattgag gacttacctc ttgaggagga ataccattcg ttggaagatt *F tttatgcagc tgcaaataga gcatacaatc agaatgccta caactgctgg attgccgcat *F gtatatatgt tttgactttg ttgctctccg cccagcaatt ttacatgaat agcagagtaa *F ctgccaacta atatttcaga tgtagtttcc caactactta aaactagtat tgaaattttc *F ccttcgatat atttgttaga ggtgttttcc ttttaataaa gttaaagttc aatttaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003795|gb|AE003795|arm:2R 14519511..14767606 *F estimated-cyto:56D9-56E5 gadfly-seqname:AE003795 *F seq_release:3 *F Length = 248096 *F Score = 264 bits (137), Expect = 5e-70 Genome Map *F Identities = 137/137 (100%) *F Strand = Plus / Minus *F Query: 9 gtcgaaatatcattttgaataagttgctcaatttgcttcgcaaagtgatttaccgattta 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65060 gtcgaaatatcattttgaataagttgctcaatttgcttcgcaaagtgatttaccgattta 65001 *F Query: 69 tcattctctattttcatatgtgtacaactaaataaatgtattgtaggcataagctaaaca 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 65000 tcattctctattttcatatgtgtacaactaaataaatgtattgtaggcataagctaaaca 64941 *F Query: 129 acacaataatgcaaaaa 145 *F ||||||||||||||||| *F Sbjct: 64940 acacaataatgcaaaaa 64924 *F Database: na_est.dros *F >GM16138.complete AY118852 *F Length = 472 *F Score = 675 bits (351), Expect = 0.0 *F Identities = 351/351 (100%) *F Strand = Plus / Plus *F Query: 9 tctgtttggggtattgtccaattggttttaatgggtctattcttctacataaatagtgta 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 107 tctgtttggggtattgtccaattggttttaatgggtctattcttctacataaatagtgta 166 *F Query: 69 gccctcattgaggacttacctcttgaggaggaataccattcgttggaagatttttatgca 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 167 gccctcattgaggacttacctcttgaggaggaataccattcgttggaagatttttatgca 226 *F Query: 129 gctgcaaatagagcatacaatcagaatgcctacaactgctggattgccgcatgtatatat 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 227 gctgcaaatagagcatacaatcagaatgcctacaactgctggattgccgcatgtatatat 286 *F Query: 189 gttttgactttgttgctctccgcccagcaattttacatgaatagcagagtaactgccaac 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 287 gttttgactttgttgctctccgcccagcaattttacatgaatagcagagtaactgccaac 346 *F Query: 249 taatatttcagatgtagtttcccaactacttaaaactagtattgaaattttcccttcgat 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 347 taatatttcagatgtagtttcccaactacttaaaactagtattgaaattttcccttcgat 406 *F Query: 309 atatttgttagaggtgttttccttttaataaagttaaagttcaatttaaaa 359 *F ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 407 atatttgttagaggtgttttccttttaataaagttaaagttcaatttaaaa 457 *F \# *F anon-EST:Posey171 = CG12187 *F >anon-EST:Posey171 *F ggcacgaggc gaaaccggaa acggaagcgc aggcgaacgc ttggagccca cctacataca *F actcgtattc gtagttaaga aacacattaa cgaagtgcta aacggaggag agagataagt *F gcagaatcat tttcgagagc taactgaatt tactcgttta ccttatagca gatgctttgt *F agctatgtaa ctcctgtcag atgggtgata acttaacatt gatttcgatc acgacacatt *F gtatagacta cctacttcgt aatcgtaact cgtaactgta actgtaactc taaccttata *F ttgtaactgt aactaatacc tgtaactgaa ttatgtacta aatttatgag aaacctttac *F aaaagtcact tgggcaggtg caaaggtccc tgaaaagagc tccctgggag atggaattgt *F gaaaaactga tgaaattttt ttagattatc cgcactaacc gccgctcgag gttgagatat *F tcatttcaaa accaatatgt ataaatttac aactcaacgc tatacgatac ttgaatatat *F atctatatac aaacagttaa tagtcactaa tgcgtatacc agcgtaccga aatgtgctag *F caacctaaat ttaactgaaa aacggctaac aaagaaatgt gaaaataaag tctctctatt *F taactgcaaa acaaaacgaa aaa *F Database: dmel_all_transcript_r320 *F >CG12187-RA type=mRNA; *F loc=3L:complement(2651232..2653522,2653694..2653913, *F 2654215..2654339,2659167..2659408,2659527..2659781, *F 2659842..2660098,2660248..2660496,2662850..2663190, *F 2665182..2665317,2670118..2670316); ID=CG12187-RA; *F name=CG12187-RA; db_xref='CG12187,FlyBase:FBgn0035367'; *F len=4315 *F Length = 4315 *F Genome Map *F Score = 1250 bits (650), Expect = 0.0 *F Identities = 657/664 (98%) *F Strand = Plus / Plus *F Query: 9 gcgaaaccggaaacggaagcgcaggcgaacgcttggagcccacctacatacaactcgtat 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3652 gcgaaaccggaaacggaagcgcaggcgaacgcttggagcccacctacatacaactcgtat 3711 *F Query: 69 tcgtagttaagaaacacattaacgaagtgctaaacggaggagagagataagtgcagaatc 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3712 tcgtagttaagaaacacattaacgaagtgctaaacggaggagagagataagtgcagaatc 3771 *F Query: 129 attttcgagagctaactgaatttactcgtttaccttatagcagatgctttgtagctatgt 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3772 attttcgagagctaactgaatttactcgtttaccttatagcagatgctttgtagctatgt 3831 *F Query: 189 aactcctgtcagatgggtgataacttaacattgatttcgatcacgacacattgtatagac 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3832 aactcctgtcagatgggtgataacttaacattgatttcgatcacgacacattgtatagac 3891 *F Query: 249 tacctacttcgtaatcgtaactcgtaactgtaactgtaactctaaccttatattgtaact 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3892 tacctacttcgtaatcgtaactcgtaactgtaactgtaactctaaccttatattgtaact 3951 *F Query: 309 gtaactaatacctgtaactgaattatgtactaaatttatgagaaacctttacaaaagtca 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3952 gtaactaatacctgtaactgaattatgtactaaatttatgagaaacctttacaaaagtca 4011 *F Query: 369 cttgggcaggtgcaaaggtccctgaaaagagctccctgggagatggaattgtgaaaaact 428 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4012 cttgggcaggtgcaaaggtccctgaaaagagctccctgggagatggaattgtgaaaaact 4071 *F Query: 429 gatgaaannnnnnnagattatccgcactaaccgccgctcgaggttgagatattcatttca 488 *F ||||||| |||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4072 gatgaaatttttttagattatccgcactaaccgccgctcgaggttgagatattcatttca 4131 *F Query: 489 aaaccaatatgtataaatttacaactcaacgctatacgatacttgaatatatatctatat 548 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4132 aaaccaatatgtataaatttacaactcaacgctatacgatacttgaatatatatctatat 4191 *F Query: 549 acaaacagttaatagtcactaatgcgtataccagcgtaccgaaatgtgctagcaacctaa 608 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4192 acaaacagttaatagtcactaatgcgtataccagcgtaccgaaatgtgctagcaacctaa 4251 *F Query: 609 atttaactgaaaaacggctaacaaagaaatgtgaaaataaagtctctctatttaactgca 668 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4252 atttaactgaaaaacggctaacaaagaaatgtgaaaataaagtctctctatttaactgca 4311 *F Query: 669 aaac 672 *F |||| *F Sbjct: 4312 aaac 4315 *F \# *F anon-EST:Posey175 = end of CG13203 ? *F >anon-EST:Posey175 *F ggcacgagct cgtgccgaat tcggcacgag ccggcgtcca cgcgtagttc tagatggtac *F cgtataccca ttgaacccgt atttactgta tggcggaact caagcttatg tggacgctga *F gcctggccaa taattgtaga cccaacgtag atccttaaca ataaagagta ctagaagaca *F cctcaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003826|gb|AE003826|arm:2R 6219987..6530078 *F estimated-cyto:47D5-47F15 gadfly-seqname:AE003826 *F seq_release:3 *F Length = 310092 *F Score = 246 bits (128), Expect = 1e-64 Genome Map *F Identities = 149/157 (94%), Gaps = 7/157 (4%) *F Strand = Plus / Plus *F Query: 31 ccggcgtccacgcgtagttctagatggtaccgtatacccattgaacccgtatttactgta 90 *F |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 221679 ccggcgtccacgcgtagttctagatggtaccgtagacccattgaacccgtatttactgta *F 221738 *F Query: 91 tggcggaactcaagcttatgtggacgctgagcctggccaataattgtagacccaacgtag 150 *F |||| ||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 221739 tggc-------aagcttatgtggacgctgagcctggccaataattgtagacccaacgtag *F 221791 *F Query: 151 atccttaacaataaagagtactagaagacacctcaaa 187 *F ||||||||||||||||||||||||||||||||||||| *F Sbjct: 221792 atccttaacaataaagagtactagaagacacctcaaa 221828 *F \# *F anon-EST:Posey178 = ? end CG31369 ? *F >anon-EST:Posey178 *F ggcacgaggg aagagctgca acagtaattt gaatttggaa tgcaccggag ggaacgagag *F cgctgcaaat tgtgtacata ttttgtctct gtctgtttcc ggtcgcctgc cattgtgtgc *F gtttgcttga gcttgtgcct gtgtgtgtga ttgtgggtgt atttcgtgta aataaatgct *F gtgcaaaaac agaaacagca acatcagaag aaacaggaat aacaagcgaa atggcaaaaa *F gtgcagttaa gcgtggaaat tgcattaaaa attgagcaaa aaaaaattgc gaaaacaaaa *F ggcggcgaaa agactaaaaa cggaacaaac aaagacgcaa aacgaaacgc tggagttaac *F gagcgctaaa aagcaaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003679|gb|AE003679|arm:3R 4269603..4486596 *F estimated-cyto:85A1-85A5 gadfly-seqname:AE003679 *F seq_release:3 *F Length = 216994 *F Score = 494 bits (257), Expect = e-139 Genome Map *F Identities = 257/257 (100%) *F Strand = Plus / Minus *F Query: 9 ggaagagctgcaacagtaatttgaatttggaatgcaccggagggaacgagagcgctgcaa 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 645 ggaagagctgcaacagtaatttgaatttggaatgcaccggagggaacgagagcgctgcaa 586 *F Query: 69 attgtgtacatattttgtctctgtctgtttccggtcgcctgccattgtgtgcgtttgctt 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 585 attgtgtacatattttgtctctgtctgtttccggtcgcctgccattgtgtgcgtttgctt 526 *F Query: 129 gagcttgtgcctgtgtgtgtgattgtgggtgtatttcgtgtaaataaatgctgtgcaaaa 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 525 gagcttgtgcctgtgtgtgtgattgtgggtgtatttcgtgtaaataaatgctgtgcaaaa 466 *F Query: 189 acagaaacagcaacatcagaagaaacaggaataacaagcgaaatggcaaaaagtgcagtt 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 465 acagaaacagcaacatcagaagaaacaggaataacaagcgaaatggcaaaaagtgcagtt 406 *F Query: 249 aagcgtggaaattgcat 265 *F ||||||||||||||||| *F Sbjct: 405 aagcgtggaaattgcat 389 *F Score = 37.2 bits (19), Expect = 0.29 Genome Map *F Identities = 19/19 (100%) *F Strand = Plus / Minus *F Query: 349 gctggagttaacgagcgct 367 *F ||||||||||||||||||| *F Sbjct: 305 gctggagttaacgagcgct 287 *F \# *F anon-EST:Posey186 = CG6549 *F >anon-EST:Posey186 *F ggcacgagct cgtgccgaat tcggcacgag ggacaatgcc aggcgaaaga acattcagca *F gtacgacgaa gtttacccca tgatggtaga ttattttgaa caggccttaa aggcacttcc *F ataaatgtct aatgtattat gtatctagaa ctatgtatgt atgtatgtaa gagcacgaat *F atgtttggaa taaacatatg catggcatcc ttctattaaa aa *F Database: dmel_all_transcript_r320 *F >CG6549-RC type=mRNA; *F loc=2L:complement(17456524..17457196,17457255..17458325, *F 17458379..17459002,17459052..17459150); ID=fws-RC; *F name=fws-RC; db_xref='CG6549,FlyBase:FBgn0024689'; *F len=2467 *F Length = 2467 *F Genome Map *F Score = 364 bits (189), Expect = e-100 *F Identities = 189/189 (100%) *F Strand = Plus / Plus *F Query: 30 gggacaatgccaggcgaaagaacattcagcagtacgacgaagtttaccccatgatggtag 89 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2279 gggacaatgccaggcgaaagaacattcagcagtacgacgaagtttaccccatgatggtag 2338 *F Query: 90 attattttgaacaggccttaaaggcacttccataaatgtctaatgtattatgtatctaga 149 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2339 attattttgaacaggccttaaaggcacttccataaatgtctaatgtattatgtatctaga 2398 *F Query: 150 actatgtatgtatgtatgtaagagcacgaatatgtttggaataaacatatgcatggcatc 209 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2399 actatgtatgtatgtatgtaagagcacgaatatgtttggaataaacatatgcatggcatc 2458 *F Query: 210 cttctatta 218 *F ||||||||| *F Sbjct: 2459 cttctatta 2467 *F \# *F anon-EST:Posey204 = CG10071 *F >anon-EST:Posey204 *F gggcacgagc aaccagaaca agaaggccca tcgcaatggc atcaagcgcc cgctgcgcaa *F acgccacgag tccactctgg gtatggatgt gaaattcctg atcaaccagc gctacgcacg *F caagggaaac ctttcccgcg aggagtccgt gaagcgctac aacgagcgca tcgcttccca *F gaagggcaag ccaaagcctg ttactctgta gatgattgcc ccgcttgtgg ataattaaag *F gacaattcag tttaacaaaa a *F Database: dmel_all_transcript_r320 *F >CG10071-RC type=mRNA; *F loc=2R:join(16337395..16337425,16337605..16337712, *F 16337782..16337961); ID=RpL29-RC; name=RpL29-RC; *F db_xref='CG10071,FlyBase:FBgn0016726'; len=319 *F Length = 319 *F Genome Map *F Score = 466 bits (241), Expect = e-131 *F Identities = 247/250 (98%) *F Strand = Plus / Plus *F Query: 18 caaccagaacaagaaggcccatcgcaatggcatcaagcgcccgctgcgcaaacgccacga 77 *F |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 67 caaccagaacaagaaggcccatcgtaatggcatcaagcgcccgctgcgcaaacgccacga 126 *F Query: 78 gtccactctgggtatggatgtgaaattcctgatcaaccagcgctacgcacgcaagggaaa 137 *F ||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||| *F Sbjct: 127 gtccactctgggtatggatgtgaaatttctgatcaaccagcgctacgcacgcaagggaaa 186 *F Query: 138 cctttcccgcgaggagtccgtgaagcgctacaacgagcgcatcgcttcccagaagggcaa 197 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 187 cctttcccgcgaggagtccgtgaagcgctacaacgagcgcatcgcttcccagaagggcaa 246 *F Query: 198 gccaaagcctgttactctgtagatgattgccccgcttgtggataattaaaggacaattca 257 *F ||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||| *F Sbjct: 247 gccaaagcctgttactctgtagatgattgccccgcgtgtggataattaaaggacaattca 306 *F Query: 258 gtttaacaaa 267 *F |||||||||| *F Sbjct: 307 gtttaacaaa 316 *F \# *F anon-EST:Posey215 = CG2985? *F >anon-EST:Posey215 *F ggcacgaggc gagtgggtat cttaagaaag actgtactta atatttaaga cactttggcc *F tgcgctttta ccaatatttt tgtggcccaa cttggcccga actccttggt tccaacaata *F ttttgcattg atatgctaga gctaccaaca agtatatagt attaatattt ctaacaattc *F ggaatgtgca cttaactaag cgaagattga aactaaaaat cagaagatgg aaacatccta *F tttaaatagg tggaagttta aataagcaaa agtctctcgc ctaagccctg atcctaaact *F cttggctgta tttctaattg ttatcgcttc atgttgaggt ttattgttat aacgccagcc *F tcccatgagc aatactcccc ccaaattacc gtaaacaaat tattttctaa tctgagccca *F agccataagt ggagaagatt agacctcaac accacaaaaa caccgacaac aataaaataa *F atctaagttt acaaaaccaa aaa *F Database: dmel_all_transcript_r320 *F >CG2985-RA type=mRNA; loc=X:join(9793758..9794044,9794122..9795724); Genome *F Map *F ID=Yp1-RA; name=Yp1-RA; *F db_xref='CG2985,FlyBase:FBgn0004045'; len=1890 *F Length = 1890 *F Score = 431 bits (224), Expect = e-120 *F Identities = 224/224 (100%) *F Strand = Plus / Minus *F Query: 278 cgcctaagccctgatcctaaactcttggctgtatttctaattgttatcgcttcatgttga 337 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1890 cgcctaagccctgatcctaaactcttggctgtatttctaattgttatcgcttcatgttga 1831 *F Query: 338 ggtttattgttataacgccagcctcccatgagcaatactccccccaaattaccgtaaaca 397 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1830 ggtttattgttataacgccagcctcccatgagcaatactccccccaaattaccgtaaaca 1771 *F Query: 398 aattattttctaatctgagcccaagccataagtggagaagattagacctcaacaccacaa 457 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1770 aattattttctaatctgagcccaagccataagtggagaagattagacctcaacaccacaa 1711 *F Query: 458 aaacaccgacaacaataaaataaatctaagtttacaaaaccaaa 501 *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1710 aaacaccgacaacaataaaataaatctaagtttacaaaaccaaa 1667 *F \# *F anon-EST:Posey216 = end of CG32423 *F >anon-EST:Posey216 *F ggcacgaggg gagaataagc ataagcttac aaagaccgag aagaaatggg gaatcttgta *F ctagttttta gcatattttt tgtgctcatc agtcgattta atgaatgaat tttttgtagc *F tgtctacagt tttttacgtt aactgttttt tagacatatt aatgaggaaa gtaaaaacca *F acaaaaacaa cctagaaata gtatattttt tctgcatatg acaagttgtt gaggaaagag *F aagaacgatg tttgattagc atggaaacca agaggcaaac actttttaaa agggaatagc *F gaaacaaacc gaggcaacac acaaaagtag cgaaacacag aaaacagaaa ataaaatttg *F aaactgtaga attgtagttt agctttgtga aggctggcga agaatgtacg attttttgaa *F ggatcagcta ggatcgaaaa gaacaacagc aacaacaaca gcagcagcaa caacaactgg *F cacaggcagt agcagaaatg tataacaaaa accaagaaaa ttaatgttaa attaaacaac *F aaaaaagaaa aaacaaaata atgaagtaag atagaagaaa ctacgtttag taaacttagt *F taataagcga agatttcttt agtcttgtaa gcgtctctta agtagagttg aacatgaagt *F tgagtatgga gtttagaatt gaaattaaac ctttgtcaaa gcctagcata tttatttttc *F cgagacacac acacacacac tgacacacaa acacagcaag acacaccaac acggacgcac *F ctatcaagcc taagcattgt atttgtgatt ttttcgaatg ttaattgatt aacgaaacac *F gatgagcaaa ataagcgaaa caagggaaac atcaagtggg cgatgtgggt gggagatagt *F gtggcgaagt gagaaatttt tgagaatctc cgtttgagca aggacaccct ctcaccacaa *F acaacattcc tgagcaaagt ctttgatttg attttgatat aaaaaaaatt tgtatgtgtt *F gatacgattt gattttcgag tttagagctg tcgagtcgcc taactgaact atctacatat *F atatatacat tatatatata tacatatata tatatataaa aacgtagaaa cacaacgcca *F aaagtcttcc taagcctaaa accaaaacaa caacataatg caaatgtttt tttttttatt *F cgattattt gctttagact gactacgaac tgaaactgta acaataacaa caaatgcagg *F attgtcaacc caaaaacaaa aa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003567|gb|AE003567|arm:3L 5083015..5237352 *F estimated-cyto:64C9-64C12 gadfly-seqname:AE003567 *F seq_release:3 *F Length = 154338 *F Score = 833 bits (433), Expect = 0.0 Genome Map *F Identities = 433/433 (100%) *F Strand = Plus / Minus *F Query: 9 gggagaataagcataagcttacaaagaccgagaagaaatggggaatcttgtactagtttt 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 36359 gggagaataagcataagcttacaaagaccgagaagaaatggggaatcttgtactagtttt 36300 *F Query: 69 tagcatattttttgtgctcatcagtcgatttaatgaatgaattttttgtagctgtctaca 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 36299 tagcatattttttgtgctcatcagtcgatttaatgaatgaattttttgtagctgtctaca 36240 *F Query: 129 gttttttacgttaactgttttttagacatattaatgaggaaagtaaaaaccaacaaaaac 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 36239 gttttttacgttaactgttttttagacatattaatgaggaaagtaaaaaccaacaaaaac 36180 *F Query: 189 aacctagaaatagtatattttttctgcatatgacaagttgttgaggaaagagaagaacga 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 36179 aacctagaaatagtatattttttctgcatatgacaagttgttgaggaaagagaagaacga 36120 *F Query: 249 tgtttgattagcatggaaaccaagaggcaaacactttttaaaagggaatagcgaaacaaa 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 36119 tgtttgattagcatggaaaccaagaggcaaacactttttaaaagggaatagcgaaacaaa 36060 *F Query: 309 ccgaggcaacacacaaaagtagcgaaacacagaaaacagaaaataaaatttgaaactgta 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 36059 ccgaggcaacacacaaaagtagcgaaacacagaaaacagaaaataaaatttgaaactgta 36000 *F Query: 369 gaattgtagtttagctttgtgaaggctggcgaagaatgtacgattttttgaaggatcagc 428 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35999 gaattgtagtttagctttgtgaaggctggcgaagaatgtacgattttttgaaggatcagc 35940 *F Query: 429 taggatcgaaaag 441 *F ||||||||||||| *F Sbjct: 35939 taggatcgaaaag 35927 *F Score = 565 bits (294), Expect = e-160 Genome Map *F Identities = 302/310 (97%) *F Strand = Plus / Minus *F Query: 767 caacacggacgcacctatcaagcctaagcattgtatttgtgattttttcgaatgttaatt 826 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35601 caacacggacgcacctatcaagcctaagcattgtatttgtgattttttcgaatgttaatt 35542 *F Query: 827 gattaacgaaacacgatgagcaaaataagcgaaacaagggaaacatcaagtgggcgatgt 886 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35541 gattaacgaaacacgatgagcaaaataagcgaaacaagggaaacatcaagtgggcgatgt 35482 *F Query: 887 gggtgggagatagtgtggcgaagtgagaaatttttgagaatctccgtttgagcaaggaca 946 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35481 gggtgggagatagtgtggcgaagtgagaaatttttgagaatctccgtttgagcaaggaca 35422 *F Query: 947 ccctctcaccacaaacaacattcctgagcaaagtctttgatttgattttgatatnnnnnn 1006 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35421 ccctctcaccacaaacaacattcctgagcaaagtctttgatttgattttgatataaaaaa 35362 *F Query: 1007 nntttgtatgtgttgatacgatttgattttcgagtttagagctgtcgagtcgcctaactg 1066 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35361 aatttgtatgtgttgatacgatttgattttcgagtttagagctgtcgagtcgcctaactg 35302 *F Query: 1067 aactatctac 1076 *F |||||||||| *F Sbjct: 35301 aactatctac 35292 *F Score = 404 bits (210), Expect = e-111 Genome Map *F Identities = 228/246 (92%) *F Strand = Plus / Minus *F Query: 478 tggcacaggcagtagcagaaatgtataacaaaaaccaagaaaattaatgttaaattaaac 537 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35890 tggcacaggcagtagcagaaatgtataacaaaaaccaagaaaattaatgttaaattaaac 35831 *F Query: 538 aacnnnnnnnnnnnnnnnnnntaatgaagtaagatagaagaaactacgtttagtaaactt 597 *F ||| ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35830 aacaaaaaagaaaaaacaaaataatgaagtaagatagaagaaactacgtttagtaaactt 35771 *F Query: 598 agttaataagcgaagatttctttagtcttgtaagcgtctcttaagtagagttgaacatga 657 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35770 agttaataagcgaagatttctttagtcttgtaagcgtctcttaagtagagttgaacatga 35711 *F Query: 658 agttgagtatggagtttagaattgaaattaaacctttgtcaaagcctagcatatttattt 717 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35710 agttgagtatggagtttagaattgaaattaaacctttgtcaaagcctagcatatttattt 35651 *F Query: 718 ttccga 723 *F |||||| *F Sbjct: 35650 ttccga 35645 *F Score = 239 bits (124), Expect = 2e-61 Genome Map *F Identities = 144/157 (91%), Gaps = 1/157 (0%) *F Strand = Plus / Minus *F Query: 1126 agaaacacaacgccaaaagtcttcctaagcctaaaaccaaaacaacaacataatgcaaat 1185 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35241 agaaacacaacgccaaaagtcttcctaagcctaaaaccaaaacaacaacataatgcaaat 35182 *F Query: 1186 gnnnnnnnnnnnatt-cgattatttgctttagactgactacgaactgaaactgtaacaat 1244 *F | ||| |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 35181 gtttttttttttattacgattatttgctttagactgactacgaactgaaactgtaacaat 35122 *F Query: 1245 aacaacaaatgcaggattgtcaacccaaaaacaaaaa 1281 *F |||||||||||||||||||| |||||||||||||||| *F Sbjct: 35121 aacaacaaatgcaggattgtaaacccaaaaacaaaaa 35085 *F \# *F anon-EST:Posey221 = CG3620 *F >anon-EST:Posey221 *F gcacgagcaa acaaacgcga acgcgaagga agcttcaaaa gagagacaag agcgtgaaaa *F ggaggtggag aggagctgcg gagaatctaa gtggctgtga gctgaaatac aaaaaaaaaa *F aaaacaagtg caaccaaata cagtgttacc tgttgtgagt aaacttaagg aaacgtggag *F agagcgagag agtgcgacag ccgactgatc aaactgtaaa tcttgcaatg ctcttcaaac *F tgaaattcgc aaggcgggaa aaaacaaaca aatataaata aacaagaata acaaacaaaa *F ataggtagca aaagcaggaa caacaaaagc cgaagccaat taattaaata catatctact *F tcagtacatt gtcgtgctgc catcaaacta aactaaatgt tgcgctgcgt cgaggatttt *F aaataaaagg gaacctgccg ctcggaagtt gtggcagaag tgcagcaagt gctgccgggc *F caagttcaat tagcccggaa aacggccatg tgatctgcta taaatgcgat tgtatataca *F cacacacaca taatcccgtt gggagcagcg atagataggg ccagtatata atatgtccgg *F aaattaagtg gactgtagaa aaa *F Database: dmel_all_transcript_r320 *F CG3620-RB type=mRNA; *F loc=X:join(4063242..4063771,4073412..4073456, *F 4089620..4090203,4090269..4090328,4094083..4094229, *F 4095087..4095166,4097656..4097892,4097961..4098127, *F 4098192..4098496,4098892..4099056,4099121..4099487, *F 4099683..4100946,4101011..4101167,4101232..4101321, *F 4102582..4103070); ID=norpA-RB; name=norpA-RB; *F db_xref='CG3620,FlyBase:FBgn0004625'; len=4687 *F Length = 4687 *F Genome Map *F Score = 414 bits (215), Expect = e-115 *F Identities = 245/264 (92%), Gaps = 1/264 (0%) *F Strand = Plus / Plus *F Query: 360 ttcagtacattgtcgtgctgccatcaaactaaactaaatgttgcgctgcgtcgaggattt 419 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 531 ttcagtacattgtcgtgctgccatcaaactaaactaaatgttgcgctgcgtcgaggattt 590 *F Query: 420 taaataaaagggaacctgccgctcggaagttgtggcagaagtgcagcaagtgctgccggg 479 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 591 taaataaaagggaacctgccgctcggaagttgtggcagaagtgcagcaagtgctgccggg 650 *F Query: 480 ccaagttcaattagcccggaaaacggccatgtgatctgctataaatgcgattgtatatnn 539 *F |||||||||||||||| | ||| | |||||||||||||||||||||||||||||||| *F Sbjct: 651 ccaagttcaattagccggaaaacggaccatgtgatctgctataaatgcgattgtatatac 710 *F Query: 540 nnnnnnnnnnntaatcccgttgggagcagcgatagatagggccagtatataatatgtccg 599 *F |||| |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 711 acacacacacataat-ccgttgggagcagcgatagatagggccagtatataatatgtccg 769 *F Query: 600 gaaattaagtggactgtagaaaaa 623 *F |||||||||||||||||||||||| *F Sbjct: 770 gaaattaagtggactgtagaaaaa 793 *F Score = 164 bits (85), Expect = 1e-39 *F Identities = 85/85 (100%) *F Strand = Plus / Plus *F Query: 8 caaacaaacgcgaacgcgaaggaagcttcaaaagagagacaagagcgtgaaaaggaggtg 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 447 caaacaaacgcgaacgcgaaggaagcttcaaaagagagacaagagcgtgaaaaggaggtg 506 *F Query: 68 gagaggagctgcggagaatctaagt 92 *F ||||||||||||||||||||||||| *F Sbjct: 507 gagaggagctgcggagaatctaagt 531 *F \# *F anon-EST:Posey226 = end of CG32712 ? *F >anon-EST:Posey226 *F ggcacgagtt tttttttttt tttttatacc taaacctatc tctttccttt ccgcgctagt *F tatttgttcc ctaaaggcat tttcatttct cgattttgtt tatgcaaatt aaaacattaa *F aaaaaaaaaa ataaacaaaa aaaaaattgt ataccaaaaa tgtgtataaa taatcgaggg *F aaagaaaagt ttgatatttc tcactaatta tatgtaattt cccttttact ttcgttttag *F ctattttgat tatgcacaag ttgaacgata tgaaagttgg tttcatgttt ttaagcacaa *F atgaaaattg aacttgatgt acgaatgagg caattcacgt ttaattggaa aacatcaatt *F ttgtttgcgt atgtacaatg attttgggtt ctgttgtggt ctggaagtac tatttcttca *F atgattgtac aattggctat cgtatctcat ttcgttttcc tatttatggg tgaaggcacg *F ccaaaacatc tagagtttg *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003444|gb|AE003444|arm:X 8154224..8453909 *F estimated-cyto:7E7-8A2 gadfly-seqname:AE003444 *F seq_release:3 *F Length = 299686 *F Score = 679 bits (353), Expect = 0.0 Genome Map *F Identities = 353/353 (100%) *F Strand = Plus / Minus *F Query: 147 ttgtataccaaaaatgtgtataaataatcgagggaaagaaaagtttgatatttctcacta 206 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15777 ttgtataccaaaaatgtgtataaataatcgagggaaagaaaagtttgatatttctcacta 15718 *F Query: 207 attatatgtaatttcccttttactttcgttttagctattttgattatgcacaagttgaac 266 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15717 attatatgtaatttcccttttactttcgttttagctattttgattatgcacaagttgaac 15658 *F Query: 267 gatatgaaagttggtttcatgtttttaagcacaaatgaaaattgaacttgatgtacgaat 326 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15657 gatatgaaagttggtttcatgtttttaagcacaaatgaaaattgaacttgatgtacgaat 15598 *F Query: 327 gaggcaattcacgtttaattggaaaacatcaattttgtttgcgtatgtacaatgattttg 386 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15597 gaggcaattcacgtttaattggaaaacatcaattttgtttgcgtatgtacaatgattttg 15538 *F Query: 387 ggttctgttgtggtctggaagtactatttcttcaatgattgtacaattggctatcgtatc 446 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15537 ggttctgttgtggtctggaagtactatttcttcaatgattgtacaattggctatcgtatc 15478 *F Query: 447 tcatttcgttttcctatttatgggtgaaggcacgccaaaacatctagagtttg 499 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15477 tcatttcgttttcctatttatgggtgaaggcacgccaaaacatctagagtttg 15425 *F Score = 179 bits (93), Expect = 6e-44 Genome Map *F Identities = 93/93 (100%) *F Strand = Plus / Minus *F Query: 26 atacctaaacctatctctttcctttccgcgctagttatttgttccctaaaggcattttca 85 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 15898 atacctaaacctatctctttcctttccgcgctagttatttgttccctaaaggcattttca 15839 *F Query: 86 tttctcgattttgtttatgcaaattaaaacatt 118 *F ||||||||||||||||||||||||||||||||| *F Sbjct: 15838 tttctcgattttgtttatgcaaattaaaacatt 15806 *F \# *F anon-EST:Posey232 = CG18815 *F >anon-EST:Posey232 *F ggcagagcca tagcactctt agaacgtacg aaagcccccg acccagcccg ttgcattcca *F actattcacg caatatggag cgcagtaaga gagtcacact gagagttata cgagtaggct *F ctctcgtagc ccaattatgg aaattatcac tgaaaaatca attcgaaaag caatcaatta *F ttatacacac acggtagcta tacctaatta aatgccaaaa tacaagcaat ttgcgaggga *F aattctccag cgcgagacgt ttcagagtat agaagcaact gactttgata tttcttcgct *F gctaattatt tcggtttact tatttgggac ttgttcgtat ttnaattgaa attaataaag *F ttgtattatc caagga *F Database: dmel_all_transcript_r320 *F >CG18815-RC type=mRNA; *F loc=3L:join(11585749..11585782,11585875..11586033, *F 11586095..11586126,11586493..11587036, *F 11587113..11587531); ID=CG18815-RC; name=CG18815-RC; *F db_xref='CG18815,FlyBase:FBgn0042138'; len=1188 *F Length = 1188 *F Genome Map *F Score = 685 bits (356), Expect = 0.0 *F Identities = 363/367 (98%) *F Strand = Plus / Plus *F Query: 8 ccatagcactcttagaacgtacgaaagcccccgacccagcccgttgcattccaactattc 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 808 ccatagcactcttagaacgtacgaaagcccccgacccagcccgttgcattccaactattc 867 *F Query: 68 acgcaatatggagcgcagtaagagagtcacactgagagttatacgagtaggctctctcgt 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 868 acgcaatatggagcgcagtaagagagtcacactgagagttatacgagtaggctctctcgt 927 *F Query: 128 agcccaattatggaaattatcactgaaaaatcaattcgaaaagcaatcaattattataca 187 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 928 agcccaattatggaaattatcactgaaaaatcaattcgaaaagcaatcaattattataca 987 *F Query: 188 cacacggtagctatacctaattaaatgccaaaatacaagcaatttgcgagggaaattctc 247 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 988 cacacggtagctatacctaattaaatgccaaaatacaagcaatttgcgagggaaattctc 1047 *F Query: 248 cagcgcgagacgtttcagagtatagaagcaactgactttgatatttcttcgctgctaatt 307 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1048 cagcgcgagacgtttcagagtatagaagcaactgactttgatatttcttcgctgctaatt 1107 *F Query: 308 atttcggtttacttatttgggacttgttcgtatttnaattgaaattaataaagttgtatt 367 *F ||||||||||||||||||||||||||||||||||| ||||||||||||||||||| ||| *F Sbjct: 1108 atttcggtttacttatttgggacttgttcgtatttaaattgaaattaataaagttatata 1167 *F Query: 368 atccaag 374 *F ||| ||| *F Sbjct: 1168 atcaaag 1174 *F \# *F anon-EST:Posey243 = CG32172 *F >anon-EST:Posey243 *F ggcacgagcg ctggcgcgcc acgccccccg cccctcccac tgtcacgcgc tcgccacgcc *F ccccagttgc cgccccatta gcagcgggca gatggacaac gtgtgggtgg cccacaagga *F catctagtaa cacgacgccc aacagcagcc gcaaggtctg atacgccgcc cgccacgccc *F atcgtgtttg ggcggcagag gaagcggtcc ggaagcggaa acggaaacgg aaacgggcga *F gcaaaatggt ggcgcggtat cgcgggcaag gcgacggcgc gtccacaaaa aataaccata *F gagacgttta aggcaaattc taaatgaaca aaagtataaa ccaaa *F Database: dmel_all_transcript_r320 *F >CG32172-RA type=mRNA; loc=3L:complement(17348426..17350337); Genome Map *F ID=noe-RA; name=noe-RA; *F db_xref='CG32172,FlyBase:FBgn0026197'; len=1912 *F Length = 1912 *F Score = 540 bits (281), Expect = e-153 *F Identities = 281/281 (100%) *F Strand = Plus / Plus *F Query: 65 agttgccgccccattagcagcgggcagatggacaacgtgtgggtggcccacaaggacatc 124 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 704 agttgccgccccattagcagcgggcagatggacaacgtgtgggtggcccacaaggacatc 763 *F Query: 125 tagtaacacgacgcccaacagcagccgcaaggtctgatacgccgcccgccacgcccatcg 184 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 764 tagtaacacgacgcccaacagcagccgcaaggtctgatacgccgcccgccacgcccatcg 823 *F Query: 185 tgtttgggcggcagaggaagcggtccggaagcggaaacggaaacggaaacgggcgagcaa 244 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 824 tgtttgggcggcagaggaagcggtccggaagcggaaacggaaacggaaacgggcgagcaa 883 *F Query: 245 aatggtggcgcggtatcgcgggcaaggcgacggcgcgtccacaaaaaataaccatagaga 304 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 884 aatggtggcgcggtatcgcgggcaaggcgacggcgcgtccacaaaaaataaccatagaga 943 *F Query: 305 cgtttaaggcaaattctaaatgaacaaaagtataaaccaaa 345 *F ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 944 cgtttaaggcaaattctaaatgaacaaaagtataaaccaaa 984 *F \# *F anon-EST:Posey246 = CG5627 *F >anon-EST:Posey246 *F ggcacgagta ttcatagagt tgattgtttt gtctttaagc atagtggtga acaaattatg *F ttacaaaatt gcaaagtttt gctgtctgta aatacacgaa gtatcaatat ggatcttaaa *F cttaagcgtg cgcataaacg tggtattgtc cttggtctct agagccaaag ttacagtatt *F ctccaatctt cctcttccgc acggatcgcg aaagccccgc gatccatcag ttatgctata *F tacatatata tatatatata tatgtaccgt aatcgattat actttagcca aatcatatca *F ttagccgcct ctcactaatc ccatagacaa attgtttcct gctaagcttt aaggcagaaa *F gcgccaaaca aaaacaaaaa ctcgcttacg actacctaaa acatagataa tgacaaatat *F atatatacat acatacatac atatatatac actcagatcg ggacttgact tcactgtggg *F gccctttgag agggcacaac aaacgaaaca caaacggaaa ttgaaggcaa tctagttagg *F gatatagtat actataccaa ctaaattcaa actaacaaac aaaatattaa aatattatgt *F gaatatctac cagacaacta cgatcataca taaagtaaat atatatatat ataaatatat *F atttatggta aaggaattta actagttcgt acagaaaata ttaagagatt gtatgacagc *F aagaagtggt cctaagttca gatctattgg gatcggtagt ttaaggaatt gtcagggaac *F acgagcacgg acgacggaga gttttggatt aacggggact ctggagagca ataagtatgt *F gtatgtgtat gtagtttgta aaaggagatc ggcgtacatc caaaatacac acatacacac *F acatatatat atatatatat ctgaaaatat atataaaaaa tataactatt tttttgttag *F ctggctctgg atgttaacca caaataaagc ccagcaccca tatatatata tatatacata *F cctctttagc cgtgtatata tgtatatgat cgattgtatt tttcaaagtt caaagcgaga *F aataaaaaaa aataaaaaca aaaa *F Database: dmel_all_transcript_r320 *F >CG5627-GEF-RA type=mRNA; *F loc=X:join(14824267..14824389,14824570..14824903, *F 14825128..14825627,14825681..14826042,14826101..14826258, *F 14828653..14829779,14829848..14830051,14830263..14830313, *F 14830827..14831196,14831257..14831642,14831704..14832186, *F 14832614..14832709,14833027..14833284,14833343..14833582, *F 14833647..14833919,14833991..14834158,14840711..14840783, *F 14840861..14840907,14842082..14844776); ID=rab3-GEF-RA; *F name=rab3-GEF-RA; db_xref='CG5627,FlyBase:FBgn0030613'; *F len=7948 *F Length = 7948 *F Genome Map *F Score = 779 bits (405), Expect = 0.0 *F Identities = 429/453 (94%) *F Strand = Plus / Plus *F Query: 450 cactcagatcgggacttgacttcactgtggggccctttgagagggcacaacaaacgaaac 509 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7297 cactcagatcgggacttgacttcactgtggggccctttgagagggcacaacaaacgaaac 7356 *F Query: 510 acaaacggaaattgaaggcaatctagttagggatatagtatactataccaactaaattca 569 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7357 acaaacggaaattgaaggcaatctagttagggatatagtatactataccaactaaattca 7416 *F Query: 570 aactaacaaacaaaatattaaaatattatgtgaatatctaccagacaactacgatcatac 629 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7417 aactaacaaacaaaatattaaaatattatgtgaatatctaccagacaactacgatcatac 7476 *F Query: 630 ataaagtaannnnnnnnnnnnnnnnnnnnnnnnttatggtaaaggaatttaactagttcg 689 *F ||||||||| ||||||||||||||||||||||||||| *F Sbjct: 7477 ataaagtaaatatatatatatataaatatatatttatggtaaaggaatttaactagttcg 7536 *F Query: 690 tacagaaaatattaagagattgtatgacagcaagaagtggtcctaagttcagatctattg 749 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7537 tacagaaaatattaagagattgtatgacagcaagaagtggtcctaagttcagatctattg 7596 *F Query: 750 ggatcggtagtttaaggaattgtcagggaacacgagcacggacgacggagagttttggat 809 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7597 ggatcggtagtttaaggaattgtcagggaacacgagcacggacgacggagagttttggat 7656 *F Query: 810 taacggggactctggagagcaataagtatgtgtatgtgtatgtagtttgtaaaaggagat 869 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7657 taacggggactctggagagcaataagtatgtgtatgtgtatgtagtttgtaaaaggagat 7716 *F Query: 870 cggcgtacatccaaaatacacacatacacacac 902 *F ||||||||||||||||||||||||||||||||| *F Sbjct: 7717 cggcgtacatccaaaatacacacatacacacac 7749 *F \# *F anon-EST:Posey247 = CG5119 *F >anon-EST:Posey247 *F ggcacgagcg atatgtaatt tttttcaatt cttttttctc aaattcaaac tttaagaaag *F ctttgtttag aaaaagaaac gcgacaacca tacactttta aaaaagcaaa gaaaaaacaa *F aaaaattgca aaagagttaa aataaaaacg aaaaaatgga aagcaaactg tgcagagaac *F agaagaatag aaagaaagaa agaaaaagaa aaccgccaga atatgagagt caagtttttg *F ctactttgaa aaaagagaga aaatacagaa taccaacagc tgaaaaaata tgttgacaaa *F tgaaaacaaa acataaatcg aaaaaactac aaaaaaatgg caaaacctat gttaaaacag *F caaa *F Database: dmel_all_transcript_r320 *F >CG5119-RG type=mRNA; *F loc=2R:join(13205133..13205406,13207147..13208631, *F 13208701..13209008,13209076..13209969); ID=pAbp-RG; *F name=pAbp-RG; db_xref='CG5119,FlyBase:FBgn0003031'; *F len=2961 *F Length = 2961 *F Genome Map *F Score = 150 bits (78), Expect = 6e-36 *F Identities = 85/92 (92%) *F Strand = Plus / Plus *F Query: 7 agcgatatgtaannnnnnncaattcttttttctcaaattcaaactttaagaaagctttgt 66 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2607 agcgatatgtaatttttttcaattcttttttctcaaattcaaactttaagaaagctttgt 2666 *F Query: 67 ttagaaaaagaaacgcgacaaccatacacttt 98 *F |||||||||||||||||||||||||||||||| *F Sbjct: 2667 ttagaaaaagaaacgcgacaaccatacacttt 2698 *F Score = 127 bits (66), Expect = 6e-29 *F Identities = 68/69 (98%) *F Strand = Plus / Plus *F Query: 213 ccgccagaatatgagagtcaagtttttgctactttgaaaaaagagagaaaatacagaata 272 *F |||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2813 ccgcaagaatatgagagtcaagtttttgctactttgaaaaaagagagaaaatacagaata 2872 *F Query: 273 ccaacagct 281 *F ||||||||| *F Sbjct: 2873 ccaacagct 2881 *F \# *F anon-EST:Posey249 = CG5119 *F >anon-EST:Posey249 *F ggcacgagcg atatgtaatt tttttcaatt cttttttctc aaattcaaac tttaagaaag *F ctttgtttag aaaaagaaac gcgacaacca tacactttta aaaaagcaaa gaaaaaacaa *F aaaaattgca aaagagttaa aataaaaacg aaaaaatgga aagcaaactg tgcagagaac *F agaagaatag aaagaaagaa agaaaaagaa aaccgcaaga atatgagagt caagtttttg *F ctactttgaa aaaagagaga aaatacagaa taccaacagc tgaaaaaata tgttgacaaa *F tgaaaacaaa acataaatcg aaaaaactac aaaaaaatgg caaaacctat gtaaaaacag *F caaa *F Database: dmel_all_transcript_r320 *F >CG5119-RG type=mRNA; *F loc=2R:join(13205133..13205406,13207147..13208631, *F 13208701..13209008,13209076..13209969); ID=pAbp-RG; *F name=pAbp-RG; db_xref='CG5119,FlyBase:FBgn0003031'; *F len=2961 *F Length = 2961 *F Genome Map *F Score = 150 bits (78), Expect = 6e-36 *F Identities = 85/92 (92%) *F Strand = Plus / Plus *F Query: 7 agcgatatgtaannnnnnncaattcttttttctcaaattcaaactttaagaaagctttgt 66 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2607 agcgatatgtaatttttttcaattcttttttctcaaattcaaactttaagaaagctttgt 2666 *F Query: 67 ttagaaaaagaaacgcgacaaccatacacttt 98 *F |||||||||||||||||||||||||||||||| *F Sbjct: 2667 ttagaaaaagaaacgcgacaaccatacacttt 2698 *F Score = 133 bits (69), Expect = 1e-30 *F Identities = 69/69 (100%) *F Strand = Plus / Plus *F Query: 213 ccgcaagaatatgagagtcaagtttttgctactttgaaaaaagagagaaaatacagaata 272 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2813 ccgcaagaatatgagagtcaagtttttgctactttgaaaaaagagagaaaatacagaata 2872 *F Query: 273 ccaacagct 281 *F ||||||||| *F Sbjct: 2873 ccaacagct 2881 *F >CG5119-RD type=mRNA; *F loc=2R:join(13205087..13205150,13205226..13205406, *F 13207147..13208631,13208701..13209008, *F 13209076..13209969); ID=pAbp-RD; name=pAbp-RD; *F db_xref='CG5119,FlyBase:FBgn0003031'; len=2932 *F Length = 2932 *F Genome Map *F Score = 150 bits (78), Expect = 6e-36 *F Identities = 85/92 (92%) *F Strand = Plus / Plus *F Query: 7 agcgatatgtaannnnnnncaattcttttttctcaaattcaaactttaagaaagctttgt 66 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2578 agcgatatgtaatttttttcaattcttttttctcaaattcaaactttaagaaagctttgt 2637 *F Query: 67 ttagaaaaagaaacgcgacaaccatacacttt 98 *F |||||||||||||||||||||||||||||||| *F Sbjct: 2638 ttagaaaaagaaacgcgacaaccatacacttt 2669 *F Score = 133 bits (69), Expect = 1e-30 *F Identities = 69/69 (100%) *F Strand = Plus / Plus *F Query: 213 ccgcaagaatatgagagtcaagtttttgctactttgaaaaaagagagaaaatacagaata 272 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2784 ccgcaagaatatgagagtcaagtttttgctactttgaaaaaagagagaaaatacagaata 2843 *F Query: 273 ccaacagct 281 *F ||||||||| *F Sbjct: 2844 ccaacagct 2852 *F \# *F anon-EST:Posey25 = CG30185 *F >anon-EST:Posey25 *F ggaacgaggt atttgttaaa aaccaaagcg aagctgatgc cgaaaagggt gaattattgg *F caaccgtttc tatagtttta tctgaaacat gtatgaatgt tttatggctt aaaaactaag *F tttcactgtc acagttaaac caaaaacaca actaaaaaaa actgttttag gtgatgaaac *F tgcgacagca gacaatagaa acttaaaaac ctacacaact ttatgcttta agattaagtg *F ggacaaacat tcacttgcca ccggcagtga atctcgtgcc gaattcggca cgaggtttct *F tgcaatttcg cacgccaaat acaataactt taatttcgct gaccatgtgt gactttgcaa *F cagtgcaaaa gatagccaac tgcctgggcg ttaatcccgg caaggtgcag ctgaatgagg *F agcaggtcgt aacgcgcacg agtggccaaa agaagacgtg gccggattcg ccacgatcct *F ggagagcctg gccagcgagt ccaagtcgga gaccgcccag aacagcaggg cgtcgcggga *F ggtggaagcc caggtgtacc agtggatcga gttctccgtg ctctacgtgg cgcccaagga *F caagtacgtg tccaagcagc ttctggcgga cttcaacaaa ctgtttgcca gcaaatccta *F cctggtgggc cacttcatca ctctagccga cctggccgtc tactatgcca tctacgatct *F tgtgaaatcc ctttcgccgg tggacaagga ggtatatttg aatctctccc gctggttcga *F tcacctccag aatcgcgcgg atgtgcacca gggcgagcca ctgctgaact tcaccaccat *F ctacctgcac ggctgggcca caggcaccca catctaagcc ccgggcgggc acccttgcat *F ccattcacat ccagtcacga atgcgacact taagttattt atttctattg catttcacaa *F tgtagttttg tgtgttgtga ttatcattta atgccaatga gagagttcag cgttggaata *F tgaagaatcg ttattgtagc ccgcaaaaaa a *F Database: dmel_all_transcript_r320 *F >CG30185-RA type=mRNA; *F loc=2R:join(18587609..18587747,18587816..18588121, *F 18588193..18588467); ID=CG30185-RA; name=CG30185-RA; *F db_xref='CG30185,FlyBase:FBgn0050185'; len=720 *F Length = 720 *F Genome Map *F Score = 1269 bits (660), Expect = 0.0 *F Identities = 698/712 (98%), Gaps = 7/712 (0%) *F Strand = Plus / Plus *F Query: 295 gtttcttgcaatttcgcacgccaaatacaataactttaatttcgctgaccatgtgtgact 354 *F ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| *F Sbjct: 9 gtttcttgcaatttcgcacgccaaacacaataactttaatttcgctgaccatgtgtgacg 68 *F Query: 355 ttgcaacagtgcaaaagatagccaactgcctgggcgttaatcccggcaaggtgcagctga 414 *F ||||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||| *F Sbjct: 69 ttgcaacagtgcagaagatagcaaactgcctgggcgttaatcccggcaaggtgcagctga 128 *F Query: 415 atgaggagcaggtcgtaacgcgcacgagtggccaaaagaaga-cgtggccggattcgcca 473 *F | ||||||||||||||||||||||||||||| |||||||||| |||||||||||||||| *F Sbjct: 129 acgaggagcaggtcgtaacgcgcacgagtgggcaaaagaagagcgtggccggattcgcct 188 *F Query: 474 cgatcctggagagcctggccagcgagtccaagtcggagaccgcccagaacagcagggcgt 533 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 189 cgatcctggagagcctggccagcgagtccaagtcggagaccgcccagaacagcagggcgt 248 *F Query: 534 cgcgggaggtggaagcccaggtgtaccagtggatcgagttctccgtgctctacgtggcgc 593 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 249 cgcgggaggtggaagcccaggtgtaccagtggatcgagttctccgtgctctacgtggcgc 308 *F Query: 594 cc------aaggacaagtacgtgtccaagcagcttctggcggacttcaacaaactgtttg 647 *F || |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 309 ccggctccaaggacaagtacgtgtccaagcagcttctggcggacttcaacaaactgtttg 368 *F Query: 648 ccagcaaatcctacctggtgggccacttcatcactctagccgacctggccgtctactatg 707 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 369 ccagcaaatcctacctggtgggccacttcatcactctagccgacctggccgtctactatg 428 *F Query: 708 ccatctacgatcttgtgaaatccctttcgccggtggacaaggaggtatatttgaatctct 767 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 429 ccatctacgatcttgtgaaatccctttcgccggtggacaaggaggtatatttgaatctct 488 *F Query: 768 cccgctggttcgatcacctccagaatcgcgcggatgtgcaccagggcgagccactgctga 827 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 489 cccgctggttcgatcacctccagaatcgcgcggatgtgcaccagggcgagccactgctga 548 *F Query: 828 acttcaccaccatctacctgcacggctgggccacaggcacccacatctaagccccgggcg 887 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 549 acttcaccaccatctacctgcacggctgggccacaggcacccacatctaagccccgggcg 608 *F Query: 888 ggcacccttgcatccattcacatccagtcacgaatgcgacacttaagttatttatttcta 947 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 609 ggcacccttgcatccattcacatccagtcacgaatgcgacacttaagttatttatttcta 668 *F Query: 948 ttgcatttcacaatgtagttttgtgtgttgtgattatcatttaatgccaatg 999 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 669 ttgcatttcacaatgtagttttgtgtgttgtgattatcatttaatgccaatg 720 *F \# *F anon-EST:Posey251 = ? *F >anon-EST:Posey251 *F ggcacgaggt ttgaattaaa tacagtaaag taaatcgaaa aaaaaacaca cttaaacaaa *F atggagtcta tgtgtaaatg cattataatt agaattgcag taaaccatac aaacagaggc *F gaaacgaaat ttaaataaaa tttttaacat tccaatgatg ctatttcttt ggtgtgctgt *F tttaaacaac aaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003726|gb|AE003726|arm:3R 15173357..15421232 *F estimated-cyto:92A2-92A10 gadfly-seqname:AE003726 *F seq_release:3 *F Length = 247876 *F Score = 321 bits (167), Expect = 3e-87 Genome Map *F Identities = 176/185 (95%) *F Strand = Plus / Plus *F Query: 9 gtttgaattaaatacagtaaagtaaatcgnnnnnnnnncacacttaaacaaaatggagtc 68 *F ||||||||||||||||||||||||||||| |||||||||||||||||||||| *F Sbjct: 112508 gtttgaattaaatacagtaaagtaaatcgaaaaaaaaacacacttaaacaaaatggagtc *F 112567 *F Query: 69 tatgtgtaaatgcattataattagaattgcagtaaaccatacaaacagaggcgaaacgaa 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 112568 tatgtgtaaatgcattataattagaattgcagtaaaccatacaaacagaggcgaaacgaa *F 112627 *F Query: 129 atttaaataaaatttttaacattccaatgatgctatttctttggtgtgctgttttaaaca 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 112628 atttaaataaaatttttaacattccaatgatgctatttctttggtgtgctgttttaaaca *F 112687 *F Query: 189 acaaa 193 *F ||||| *F Sbjct: 112688 acaaa 112692 *F \# *F anon-EST:Posey259 =CG8177 *F >anon-EST:Posey259 *F ggcacgagct cgtgccgaat tcggcacgag gagcgtcaca caatgattgc atcacacgca *F aaagaaacca acgagcggaa caagttatta actcggttgg agactcgcat gtattattag *F caataattgc atttgcatat acacaaaact attataaatt tggcttagac ctatcaaatc *F gaagcaatcc cagcgtggaa aactaagagg aacccgtaaa ctatttaact tcaactggcg *F tagtgataat tagtgtcaaa cacacacaaa aacctcccct ttaagatcga acgaacaatt *F gtttataaca aaaaaatcgt tttaagcact tcaaccggag ctttctttta aagtaaatgc *F ttcgcgtttg aagtggccgc tgtaagtttg ccttctttgt acttgtagtc nggatattga *F aaaacaacca aa *F Database: dmel_all_transcript_r320 *F >CG8177-RE type=mRNA; *F loc=3L:join(9731802..9732018,9736975..9737122, *F 9737181..9737436,9737652..9737781,9738065..9738226, *F 9738295..9738537,9738607..9738747,9739232..9739432, *F 9740138..9740332,9740403..9742156,9742226..9742420, *F 9742481..9742709,9742771..9743794); ID=CG8177-RE; *F name=CG8177-RE; db_xref='CG8177,FlyBase:FBgn0036043'; *F len=4895 *F Length = 4895 *F Genome Map *F Score = 740 bits (385), Expect = 0.0 *F Identities = 395/404 (97%) *F Strand = Plus / Plus *F Query: 29 aggagcgtcacacaatgattgcatcacacgcaaaagaaaccaacgagcggaacaagttat 88 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4470 aggagcgtcacacaatgattgcatcacacgcaaaagaaaccaacgagcggaacaagttat 4529 *F Query: 89 taactcggttggagactcgcatgtattattagcaataattgcatttgcatatacacaaaa 148 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4530 taactcggttggagactcgcatgtattattagcaataattgcatttgcatatacacaaaa 4589 *F Query: 149 ctattataaatttggcttagacctatcaaatcgaagcaatcccagcgtggaaaactaaga 208 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4590 ctattataaatttggcttagacctatcaaatcgaagcaatcccagcgtggaaaactaaga 4649 *F Query: 209 ggaacccgtaaactatttaacttcaactggcgtagtgataattagtgtcaaacacacaca 268 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4650 ggaacccgtaaactatttaacttcaactggcgtagtgataattagtgtcaaacacacaca 4709 *F Query: 269 aaaacctcccctttaagatcgaacgaacaattgtttataacnnnnnnntcgttttaagca 328 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||| *F Sbjct: 4710 aaaacctcccctttaagatcgaacgaacaattgtttataacaaaaaaatcgttttaagca 4769 *F Query: 329 cttcaaccggagctttcttttaaagtaaatgcttcgcgtttgaagtggccgctgtaagtt 388 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4770 cttcaaccggagctttcttttaaagtaaatgcttcgcgtttgaagtggccgctgtaagtt 4829 *F Query: 389 tgccttctttgtacttgtagtcnggatattgaaaaacaaccaaa 432 *F |||||||||||||||||||||| ||||||||||||||||| ||| *F Sbjct: 4830 tgccttctttgtacttgtagtcaggatattgaaaaacaacaaaa 4873 *F \# *F anon-EST:Posey262 = CG15209 *F >anon-EST:Posey262 *F ggcacgaggg atgacccatg cagatataca tactatggca actatatgat attctccgac *F ctgactttcg ttagaacatt ataaggagca ttgtagttcc agcagcaatt ctttcaaata *F atagtttgcc tcatttttag actcgtatag aaatgcaaat aaactaaacc gtattattgt *F aaaaa *F Database: dmel_all_transcript_r320 *F >CG15209-RA type=mRNA; loc=X:10584094..10584942; ID=CG15209-RA; Genome Map *F name=CG15209-RA; db_xref='CG15209,FlyBase:FBgn0030237'; *F len=849 *F Length = 849 *F Score = 323 bits (168), Expect = 3e-88 *F Identities = 172/174 (98%) *F Strand = Plus / Plus *F Query: 8 gggatgacccatgcagatatacatactatggcaactatatgatattctccgacctgactt 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 669 gggatgacccatgcagatatacatactatggcaactatatgatattctccgacctgactt 728 *F Query: 68 tcgttagaacattataaggagcattgtagttccagcagcaattctttcaaataatagttt 127 *F || ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||| *F Sbjct: 729 tctttagaacattataaggagcattgtaattccagcagcaattctttcaaataatagttt 788 *F Query: 128 gcctcatttttagactcgtatagaaatgcaaataaactaaaccgtattattgta 181 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 789 gcctcatttttagactcgtatagaaatgcaaataaactaaaccgtattattgta 842 *F \# *F anon-EST:Posey263 = ? end of l(3)82Fd ? *F >anon-EST:Posey263 *F ggcacgaggt aagaccctcc ctaagcacgc attaaaccaa atccaaatca agttgaatcc *F ctgttgtatt gtgaaaacgg aagacattat tttgaggaat tttgagagca aaagctatga *F taaccggaaa tcgaaagatc atttagcttt gaaatcactt caactacacg ctaacttcat *F tcaaaaactt gagaccaaaa tacgtttact acaatatatg gcatatacat atggcactac *F agagtggcaa caactctacg gttggagcat gatatcatta aatattatcg atacgtatac *F cgcagaatag tttgttgaca accagtcaat tatgtcccaa acaaaactca ctcacgcgag *F ctaaaagctt aatggaatac atatgtattg tatatgaagt caagaagtgt ttatttccgt *F agactatgaa actatgttgt tgaattcgta gctattttgt tgtgcgttga gcagtaatgc *F agtgaatatg caagccatat gaagccgaaa ttaatgaaga taattttata ttgtaacttc *F tgtcaattaa attgtagact ggtgcatata ccaagcgaaa ctgcgtaaca tatgccgcat *F acatacatat gtatagctgt tgcagcaggc acttgtttaa gccatcaatg catggtttat *F tgatgacgaa ctatagttct gctcccccaa attttgttgt gcgttcaata agtgcttagt *F attggtgtaa tatataattc aggcagacat acaaacataa gtacccagat gtatatcaca *F gccagttaat cagttagtgt gcaattatcc cagcccatgc gagtactttt tgcactcagt *F gtccgcattt ccagtgcgct tgcccattac agtgttgagt agtttgctaa agtttctagt *F tgttgtgcac tgaaaataaa cacaatgtaa ttcgcacaat tcgcggcaga tattcggcca *F ggtatgcttc agatatgcat ataatataca catacatatg taccccttct tagagataga *F tttgcgattg ttaggtgctg aagacgacct ccgctttttc agttcgaccc tgtagaatgc *F tgattgtaga accgcgcgat tgtataaact ccacgtagaa gggagcaaca ctctatctat *F ccaggccaca acttaatgtc catgccacat gccacacatg tatgttaagt gggtgactgg *F cgagaggaa ggattttaca aaggatacag ataaatcgat cggagattga ggcagttgga *F tgtggatgca gcaggtggtt tatttttcta ccgacagatt gcaagcaatt gagaacatag *F ttgattacta gacgaacagc agccattacc taagtcgtgt gtatatatac gagaggcata *F tatctaccta aatgcataca tatatagtat atatatatat ttttggaaag cgaatctagt *F gtgtctcgat gatatcggct agctgtattc aggtcgcggc caagtactaa gcatatttgc *F aaggatagtg atcagattaa atgttaaaac taattcgaga gttaaaaagt tcaccttggt *F tttttggttt gaatggaatg cattcgttgt tttttggtta ttctaaacac ggattaattc *F ctgtgtgcaa cttgtatcgt acctataaaa ttgcattcca acaaaaacaa aaacccatga *F gcagaacgca agagtatata tataaatata tcctatatgt aaatgtagtc attacaaaaa *F tgaacaactt ttctgaaaaa taaaatgtat attcaaatat caaaaa *F Database: dmel_all_scaffolds_r310 *F gadfly|SEG:AE003603|gb|AE003603|arm:3R 971101..1266389 *F estimated-cyto:82F1-83A3 gadfly-seqname:AE003603 *F seq_release:3 *F Length = 295289 *F Score = 2732 bits (1421), Expect = 0.0 Genome Map *F Identities = 1488/1539 (96%), Gaps = 3/1539 (0%) *F Strand = Plus / Minus *F Query: 247 gcaacaactctacggttggagcatgatatcattaaatattatcgatacgtataccgcaga 306 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 126271 gcaacaactctacggttggagcatgatatcattaaatattatcgatacgtataccgcaga *F 126212 *F Query: 307 atagtttgttgacaaccagtcaattatgtcccaaacaaaactcactcacgcgagctaaaa 366 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 126211 atagtttgttgacaaccagtcaattatgtcccaaacaaaactcactcacgcgagctaaaa *F 126152 *F Query: 367 gcttaatggaatacatatgtattgtatatgaagtcaagaagtgtttatttccgtagacta 426 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 126151 gcttaatggaatacatatgtattgtatatgaagtcaagaagtgtttatttccgtagacta *F 126092 *F Query: 427 tgaaactatgttgttgaattcgtagctattttgttgtgcgttgagcagtaatgcagtgaa 486 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 126091 tgaaactatgttgttgaattcgtagctattttgttgtgcgttgagcagtaatgcagtgaa *F 126032 *F Query: 487 tatgcaagccatatgaagccgaaattaatgaagataattttatattgtaacttctgtcaa 546 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 126031 tatgcaagccatatgaagccgaaattaatggagataattttatattgtaacttctgtcaa *F 125972 *F Query: 547 ttaaattgtagactggtgcatataccaagcgaaactgcgtaacatatgccgcatacatac 606 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125971 ttaaattgtagactggtgcatataccaagcgaaactgcgtaacatatgccgcatacatac *F 125912 *F Query: 607 atatgtatagctgttgcagcaggcacttgtttaagccatcaatgcatggtttattgatga 666 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125911 atatgtatagctgttgcagcaggcacttgtttaagccatcaatgcatggtttattgatga *F 125852 *F Query: 667 cgaactatagttctgctcccccaaattttgttgtgcgttcaataagtgcttagtattggt 726 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125851 cgaactatagttctgctcccccaaattttgttgtgcgttcaataagtgcttagtattggt *F 125792 *F Query: 727 gtaatatataattcaggcagacatacaaacataagtacccagatgtatatcacagccagt 786 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125791 gtaatatataattcaggcagacatacaaacataagtacccagatgtatatcacagccagt *F 125732 *F Query: 787 taatcagttagtgtgcaattatcccagcccatgcgagtactttttgcactcagtgtccgc 846 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125731 taatcagttagtgtgcaattatcccagcccatgcgagtactttttgcactcagtgtccgc *F 125672 *F Query: 847 atttccagtgcgcttgcccattacagtgttgagtagtttgctaaagtttctagttgttgt 906 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 125671 atttccagtgcgcttgcccattacagtgttgagtagtttgccaaagtttctagttgttgt *F 125612 *F Query: 907 gcactgaaaataaacacaatgtaattcgcacaattcgcggcagatattcggccaggtatg 966 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125611 gcactgaaaataaacacaatgtaattcgcacaattcgcggcagatattcggccaggtatg *F 125552 *F Query: 967 cttcagatatgcatataatatacacatacatatgtaccccttcttagagatagatttgcg 1026 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125551 cttcagatatgcatataatatacacatacatatgtaccccttcttagagatagatttgcg *F 125492 *F Query: 1027 attgttaggtgctgaagacgacctccgctttttcagttcgaccctgtagaatgctgattg 1086 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125491 attgttaggtgctgaagacgacctccgctttttcagttcgaccctgtagaatgctgattg *F 125432 *F Query: 1087 tagaaccgcgcgattgtataaactccacgtagaagggagcaacactctatctatccaggc 1146 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| *F Sbjct: 125431 tagaaccgcgcgattgtataaactccacgtagaagggagcaccactctatctatccaggc *F 125372 *F Query: 1147 cacaacttaatgtccatgccacatgccacacatgtatgttaagtgggtgactgg-cgaga 1205 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 125371 cacaacttaatgtccatgccacatgccacacatgtatgttaagtgggtgactggacgaga *F 125312 *F Query: 1206 ggaaggattttacaaaggatacagataaatcgatcggagattgaggcagttggatgtgga 1265 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125311 ggaaggattttacaaaggatacagataaatcgatcggagattgaggcagttggatgtgga *F 125252 *F Query: 1266 tgcagcaggtggtttatttttctaccgacagattgcaagcaattgagaacatagttgatt 1325 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125251 tgcagcaggtggtttatttttctaccgacagattgcaagcaattgagaacatagttgatt *F 125192 *F Query: 1326 actagacgaacagcagccattacctaagtcgtgtgtatatatacgagaggcatatatcta 1385 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125191 actagacgaacagcagccattacctaagtcgtgtgtatatatacgagaggcatatatcta *F 125132 *F Query: 1386 cctaaatgcatacnnnnnnnnnnnnnnnnnnnnnttttggaaagcgaatctagtgtgtct 1445 *F ||||||||||||| |||||||||||||||||||||||||| *F Sbjct: 125131 cctaaatgcatacatatatagtatatatatatatttttggaaagcgaatctagtgtgtct *F 125072 *F Query: 1446 cgatgatatcggctagctgtattcaggtcgcggccaagtactaagcatatttgcaaggat 1505 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125071 cgatgatatcggctagctgtattcaggtcgcggccaagtactaagcatatttgcaaggat *F 125012 *F Query: 1506 agtgatcagattaaatgttaaaactaattcgagagttaaaaagttcaccttggttttttg 1565 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 125011 agtgatcagattaaatgttaaaactaattcgagagttaaaaagttcaccttggttttttg *F 124952 *F Query: 1566 gtttgaatggaatgcattcgttgttttttggttattctaaacacggattaattcctgtgt 1625 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 124951 gtttgaatggaatgcattcgttgttttttggttattctaaacacggattaattcctgtgt *F 124892 *F Query: 1626 gcaacttgtatcgtacctataaaattgcattccaacaaaaacaaaaacccatgagcagaa 1685 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 124891 gcaacttgtatcgtacctataaaattgcattccaacaaaaacaaaaacccatgagcagaa *F 124832 *F Query: 1686 cgcaagagnnnnnnnnnnnnnnnnnnnnnnnngtaaatgt--agtcattacaaaaatgaa 1743 *F |||||||| |||||||| |||||||||||||||||| *F Sbjct: 124831 cgcaagagtatatatataaatatatcctatatgtaaatgtaaagtcattacaaaaatgaa *F 124772 *F Query: 1744 caacttttctgaaaaataaaatgtatattcaaatatcaa 1782 *F ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 124771 caacttttctgaaaaataaaatgtatattcaaatatcaa 124733 *F \# *F anon-EST:Posey264 = not in genome, and a pretty odd seq *F >anon-EST:Posey264 *F ggcacgagag agaagagaag agaagagaag agaagagaag agaagagaag agaagagaag *F agaagagaag agaagagaag agaagagaag agaagagaag agaagagaag agaaaaa *F \# *F anon-EST:Posey268 = ? *F >anon-EST:Posey268 *F ggcacgagtc gaaacccact taatttattt gcatttggcg acattgacgg tagactattc *F agcgcgaaac tccaacatcc gttaggagtg actttcaatg ataccaataa caaactctat *F gttgctgata cctataatca taaaatcaaa attattgaca tcgattcaaa tgatatatct *F actctacaaa ttaagagcca agaaaatact aacctgattt taaacgagcc cgccggactg *F tgcttggatg ccagcggacg gaatttgttg gtagctgata ccaataacca ttcaatacat *F acaatagatt tggtaacgct tatagcacaa ccgtttggct tagatttcag acaaatagcg *F tccgctagtg aaattgatgc gccacaagat acacaaaagc ctacggaaaa taatatagtt *F aaagcattgc ctcttgattt aattaaacca agtaaaattt tttttaacct tcggctttcg *F cccagattta actttacaaa ggaagctcct caaaaatgga tagtgaaaac tgtatgtcaa *F tctgtaatag taaatccaac gtgcggaaat ctacttgatg ggatgtgcca catgcaggtg *F caggctacta ggcctgattt tatgtgtgaa agcagtcaaa tatttaccat cgaattcgta *F ttaaatttat gcctttcaaa ttgttgccta gtaaaaaaaa tcacggtttc tataaaacgc *F gatgacatac aagaagaata tatatccatc cacaacgtaa atattgacat tgaacaataa *F aaaatgtttt gatagaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:2R_wgs3_centromere_extension|gb|2R_wgs3_centromere_extension|a *F rm:2h 1296910,1651714 estimated-cyto:? *F gadfly-seqname:2R_wgs3_centromere_extension seq_release:3 *F Length = 354805 *F Score = 1435 bits (746), Expect = 0.0 Genome Map *F Identities = 762/778 (97%) *F Strand = Plus / Minus *F Query: 22 aatttatttgcatttggcgacattgacggtagactattcagcgcgaaactccaacatccg 81 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280727 aatttatttgcatttggcgacattgacggtagactattcagcgcgaaactccaacatccg *F 280668 *F Query: 82 ttaggagtgactttcaatgataccaataacaaactctatgttgctgatacctataatcat 141 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280667 ttaggagtgactttcaatgataccaataacaaactctatgttgctgatacctataatcat *F 280608 *F Query: 142 aaaatcaaaattattgacatcgattcaaatgatatatctactctacaaattaagagccaa 201 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280607 aaaatcaaaattattgacatcgattcaaatgatatatctactctacaaattaagagccaa *F 280548 *F Query: 202 gaaaatactaacctgattttaaacgagcccgccggactgtgcttggatgccagcggacgg 261 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280547 gaaaatactaacctgattttaaacgagcccgccggactgtgcttggatgccagcggacgg *F 280488 *F Query: 262 aatttgttggtagctgataccaataaccattcaatacatacaatagatttggtaacgctt 321 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280487 aatttgttggtagctgataccaataaccattcaatacatacaatagatttggtaacgctt *F 280428 *F Query: 322 atagcacaaccgtttggcttagatttcagacaaatagcgtccgctagtgaaattgatgcg 381 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280427 atagcacaaccgtttggcttagatttcagacaaatagcgtccgctagtgaaattgatgcg *F 280368 *F Query: 382 ccacaagatacacaaaagcctacggaaaataatatagttaaagcattgcctcttgattta 441 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280367 ccacaagatacacaaaagcctacggaaaataatatagttaaagcattgcctcttgattta *F 280308 *F Query: 442 attaaaccaagtaaaannnnnnnnaaccttcggctttcgcccagatttaactttacaaag 501 *F |||||||||||||||| |||||||||||||||||||||||||||||||||||| *F Sbjct: 280307 attaaaccaagtaaaattttttttaaccttcggctttcgcccagatttaactttacaaag *F 280248 *F Query: 502 gaagctcctcaaaaatggatagtgaaaactgtatgtcaatctgtaatagtaaatccaacg 561 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280247 gaagctcctcaaaaatggatagtgaaaactgtatgtcaatctgtaatagtaaatccaacg *F 280188 *F Query: 562 tgcggaaatctacttgatgggatgtgccacatgcaggtgcaggctactaggcctgatttt 621 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280187 tgcggaaatctacttgatgggatgtgccacatgcaggtgcaggctactaggcctgatttt *F 280128 *F Query: 622 atgtgtgaaagcagtcaaatatttaccatcgaattcgtattaaatttatgcctttcaaat 681 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280127 atgtgtgaaagcagtcaaatatttaccatcgaattcgtattaaatttatgcctttcaaat *F 280068 *F Query: 682 tgttgcctagtnnnnnnnntcacggtttctataaaacgcgatgacatacaagaagaatat 741 *F ||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280067 tgttgcctagtaaaaaaaatcacggtttctataaaacgcgatgacatacaagaagaatat *F 280008 *F Query: 742 atatccatccacaacgtaaatattgacattgaacaataaaaaatgttttgatagaaaa 799 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280007 atatccatccacaacgtaaatattgacattgaacaataaaaaatgttttgatagaaaa 279950 *F Score = 33.4 bits (17), Expect = 9.0 Genome Map *F Identities = 21/23 (91%) *F Strand = Plus / Minus *F Query: 406 gaaaataatatagttaaagcatt 428 *F ||||||| ||| ||||||||||| *F Sbjct: 95737 gaaaatattattgttaaagcatt 95715 *F \# *F anon-EST:Posey277 = CG31451 *F >anon-EST:Posey277 *F ggcacgaggt cgagtgcgga tcccttgccc cagcggctat tcgcctgcgc actcggtcca *F tccctggctc actacacgaa gcgacggcgt ggcccagttg ggtgcgggaa tagccggatc *F tggccttaaa aaccggataa accattgagc gcgggacgca ctcggctgta ggtacgccgc *F ccaaacaagg tcacaccgag agacacacgt ccacgaacag accagagttc ttcccgcaat *F ccatcagaca cgcactgcca tggaaaggtc acggaaatcg catggcagtg ctcaccaaaa *F tccaaatcca agcccaatcc gtccctggtc ccgccccttg cgaatccgct tgcggaattg *F tgggggtggg gtgtggggcg gctgggtggg agtttgatca taacacaaac acgaaaccaa *F accaaacacc accacccttt gcgttcggca aatcacctcc ggctgcgatt aaggggctca *F gtgccagggg ttagtcggag cacactcgac cgatttccgt gacatacgtc tctagccaaa *F tccggcagag atcggagggc gcgcccgacg ccaccagtcg tgggctatgc aatggattga *F acgaaacatg cgccagagcc aatttttata tttgaccatt aagaaaaacc tatgattttg *F ctttactatt cgacccacta tcatcccaga ttgcgtagcc tccggcgtcc ttggcgacgc *F ggcgtcgtcg tccggaccat cctccggcac gccttccaaa attggcgttc actggatcga *F ttagccccca gcaaattgaa tcgcagcccg caggagattt ggttattatc gcctcatttc *F gtacaaagag aaaccgcaac aaggaaacac tgcacatcgt tttttacacc cttgcgttat *F gttttttgaa tgtatttaaa aacaaaaaca aaatgaaaaa ccaaaaccaa ataatgaaaa *F acaaaatatg aaaacacaca aaacactgaa aaaaaaaacc aaaaatagaa tgtaagttta *F aataatatgc tattcaaaga aatccaaaac atgattaccc ataaaacaaa aa *F Database: dmel_all_transcript_r320 *F >CG31451-RA type=mRNA; loc=3R:19799725..19800949; ID=CG31451-RA; Genome Map *F name=CG31451-RA; db_xref='CG31451,FlyBase:FBgn0051451'; *F len=1225 *F Length = 1225 *F Score = 985 bits (512), Expect = 0.0 *F Identities = 522/527 (99%) *F Strand = Plus / Plus *F Query: 391 agtttgatcataacacaaacacgaaaccaaaccaaacaccaccaccctttgcgttcggca 450 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 544 agtttgatcataacacaaacacgaaaccaaaccaaacaccaccaccctttgcgttcggca 603 *F Query: 451 aatcacctccggctgcgattaaggggctcagtgccaggggttagtcggagcacactcgac 510 *F ||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||| *F Sbjct: 604 aatcacctccggctgcgattaaggggctcagtgccaggggttaatcggagcacactcgac 663 *F Query: 511 cgatttccgtgacatacgtctctagccaaatccggcagagatcggagggcgcgcccgacg 570 *F ||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 664 cgatttccgtgacatacgtctctagccaaatgcggcagagatcggagggcgcgcccgacg 723 *F Query: 571 ccaccagtcgtgggctatgcaatggattgaacgaaacatgcgccagagccaatttttata 630 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 724 ccaccagtcgtgggctatgcaatggattgaacgaaacatgcgccagagccaatttttata 783 *F Query: 631 tttgaccattaagaaaaacctatgattttgctttactattcgacccactatcatcccaga 690 *F |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||| *F Sbjct: 784 tttgaccattaagaaaaacctatgattttgctttacgattcgacccactatcatcccaga 843 *F Query: 691 ttgcgtagcctccggcgtccttggcgacgcggcgtcgtcgtccggaccatcctccggcac 750 *F ||||||||||||||||||||||||||||||| ||||||||||||||||||||||| |||| *F Sbjct: 844 ttgcgtagcctccggcgtccttggcgacgcgacgtcgtcgtccggaccatcctccagcac 903 *F Query: 751 gccttccaaaattggcgttcactggatcgattagcccccagcaaattgaatcgcagcccg 810 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 904 gccttccaaaattggcgttcactggatcgattagcccccagcaaattgaatcgcagcccg 963 *F Query: 811 caggagatttggttattatcgcctcatttcgtacaaagagaaaccgcaacaaggaaacac 870 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 964 caggagatttggttattatcgcctcatttcgtacaaagagaaaccgcaacaaggaaacac 1023 *F Query: 871 tgcacatcgttttttacacccttgcgttatgttttttgaatgtattt 917 *F ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1024 tgcacatcgttttttacacccttgcgttatgttttttgaatgtattt 1070 *F \# *F anon-EST:Posey280 = ? *F >anon-EST:Posey280 *F ggcacgaggg gaagtagcat atccattcta aacccacaac tcctcaatca cacacactat *F tgtaaacaat aacacacaca ataatgccta agtgcgaaat gtaatcaacg gtcgagagcc *F cttcgattaa gctcattgct catttacgca gagcaaagtg caaaagtccg aagtccaaaa *F tccagactct gtggcgagcc aacgtccacg tcccctaaac atccgatttt tacgacttta *F ttctcttaaa cttcgctctt aagctatccc aatctgccta tataaaccca aacatgtccc *F catatagtag ttcagttctg ggtacgttat caatcgcgat aaccctgctn agcccacttc *F aacttcgaag tccattatct acctagtgat aatccaaagg gcctagctct tcctttattg *F gaattaaaaa gaaaacgttt aataatacat aa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003788|gb|AE003788|arm:2R 1..217015 *F estimated-cyto:41E3-41E5 gadfly-seqname:AE003788 *F seq_release:3 *F Length = 217015 *F Score = 285 bits (148), Expect = 8e-76 Genome Map *F Identities = 205/224 (91%), Gaps = 8/224 (3%) *F Strand = Plus / Plus *F Query: 235 actttattctcttaaacttcgctcttaagctatcccaatctgcctatataaacccaaaca 294 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 179821 actttattatcttaaacttcgctcttaagctatcccaatctgcctatataaacccaaaca *F 179880 *F Query: 295 tgtccccata---tagt--agttcagttctgggtacgttatcaatcgcgataaccctgct 349 *F |||||||||| |||| |||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 179881 tgtccccatacactagttcagttcagttctgagtacgttatcaatcgcgataaccctgct *F 179940 *F Query: 350 nagcccacttcaacttcgaagtccattatcta-cctagtgataatccaaagggcctagct 408 *F |||||||||||| |||||||||| ||||| | ||||||||||||||||||| |||| | *F Sbjct: 179941 aagcccacttcaatttcgaagtccgttatccagcctagtgataatccaaaggctctagtt *F 180000 *F Query: 409 cttcctttattggaattaaaaagaaaacgtttaataatacataa 452 *F ||||||||||||||||||| | |||||||| |||||||||||| *F Sbjct: 180001 cttcctttattggaattaata--aaaacgttaaataatacataa 180042 *F Score = 117 bits (61), Expect = 2e-25 Genome Map *F Identities = 71/76 (93%) *F Strand = Plus / Plus *F Query: 152 agcaaagtgcaaaagtccgaagtccaaaatccagactctgtggcgagccaacgtccacgt 211 *F |||||||||||||||||||||||||||| | |||||||||||||| ||||||||||||| *F Sbjct: 179605 agcaaagtgcaaaagtccgaagtccaaagtgtagactctgtggcgacccaacgtccacgt *F 179664 *F Query: 212 cccctaaacatccgat 227 *F |||||||| ||||||| *F Sbjct: 179665 cccctaaaaatccgat 179680 *F \# *F anon-EST:Posey285 = CG6869 *F >anon-EST:Posey285 *F ggcacgaggt gccgaccatt gtaacatata caataccaga tgcataagag aacgtagaaa *F cctagtctag ttatatatgg atatacaagt gctatgtata catagttcgg taatttatat *F agttgtttaa actaaaagca agtccaaaat agaccgtaat gagtaaatga aaggcaagtt *F aaagtagttc aacwttaaaa ggcttaactc aaatgagagg aggcatccag ctatatagat *F tagatctttt gtaagtgttt ttgtaagcaa ccagcaaaca acacaatggc ttgacctgaa *F ataagttgcc tgttattaga ggcgcacttt cttttccagg tttattttct gttttaggct *F ctatctagac cggcttttag ttgaaatatt agctgttgag agctacttgt aaaggtttcc *F cagcctgttg actaactact aactttataa ccgattcgca caagccaaca tatgtatwtc *F taatataaat acaaatacaa tctgcattcc tatttttcat gaaatatcat atggcgtatt *F attttagttc gaacagcgca atgtgtgtgt tttttacaat ttttatcaat atttgtattt *F attgtataac attttattta taagtattga ataatatcat a *F Database: dmel_all_transcript_r320 *F >CG6869-RA type=mRNA; *F loc=3L:join(15096019..15096281,15099691..15099880, *F 15100347..15101063,15101249..15101432, *F 15101499..15103276); ID=FucTA-RA; name=FucTA-RA; *F db_xref='CG6869,FlyBase:FBgn0036485'; len=3132 *F Length = 3132 *F Genome Map *F Score = 1092 bits (567), Expect = 0.0 *F Identities = 617/634 (97%), Gaps = 6/634 (0%) *F Strand = Plus / Plus *F Query: 9 gtgccgaccattgtaacatatacaataccagatgcataagagaacgtagaaacctagtct 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2405 gtgccgaccattgtaacatatacaataccagatgcataagagaacgtagaaacctagtct 2464 *F Query: 69 agttatatatggatatacaagtgctatgtatacatagttcggtaatttatatagttgttt 128 *F || ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2465 agatatatatggatatacaagtgctatgtatacatagttcggtaatttatatagttgttt 2524 *F Query: 129 aaactaaaagcaagtccaaaatagaccgtaatgagtaaatgaaaggcaagttaaagtagt 188 *F |||||||||||||||||||||||||||||||||| ||||||| ||||||||||||||||| *F Sbjct: 2525 aaactaaaagcaagtccaaaatagaccgtaatgattaaatgacaggcaagttaaagtagt 2584 *F Query: 189 tcaacwttaaaaggcttaactcaaatgagaggaggcatccagctatatagattagatctt 248 *F ||||| ||||||||||||||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 2585 tcaacattaaaaggcttaactcaaatgagaggaggcatc-agctatatagattagatctt 2643 *F Query: 249 ttgtaagtgtttttgtaagcaaccagcaaacaacacaatggcttgacctgaaataagttg 308 *F |||||||||||||||||||||||||||||||| |||||||| |||||||||||||||| *F Sbjct: 2644 ttgtaagtgtttttgtaagcaaccagcaaaca---caatggctcgacctgaaataagttg 2700 *F Query: 309 cctgttattagaggcgcactttcttttccaggttta-ttttctgttttaggctctatcta 367 *F ||||||||||||| |||||||||||||||||||||| |||||||| |||||||||||||| *F Sbjct: 2701 cctgttattagag-cgcactttcttttccaggtttaattttctgtattaggctctatcta 2759 *F Query: 368 gaccggcttttagttgaaatattagctgttgagagctacttgtaaaggtttcccagcctg 427 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2760 gaccggcttttagttgaaatattagctgttgagagctacttgtaaaggtttcccagcctg 2819 *F Query: 428 ttgactaactactaactttataaccgattcgcacaagccaacatatgtatwtctaatata 487 *F ||||||||||||||||||||||||||||||||||||||| |||||||||| ||| ||||| *F Sbjct: 2820 ttgactaactactaactttataaccgattcgcacaagcctacatatgtatatctgatata 2879 *F Query: 488 aatacaaatacaatctgcattcctatttttcatgaaatatcatatggcgtattattttag 547 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||| *F Sbjct: 2880 aatacaaatacaatctgcattcctatttttcatgaaatatcatatggcgtattatcttag 2939 *F Query: 548 ttcgaacagcgcaatgtgtgtgttttttacaatttttatcaatatttgtatttattgtat 607 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | *F Sbjct: 2940 ttcgaacagcgcaatgtgtgtgttttttacaatttttatcaatatttgtatttattgtgt 2999 *F Query: 608 aacattttatttataagtattgaataatatcata 641 *F |||||||||||||||||||||||||||||||||| *F Sbjct: 3000 aacattttatttataagtattgaataatatcata 3033 *F \# *F anon-EST:Posey288 = CG13993 *F >anon-EST:Posey288 *F ggtgttgcaa ttttcagtga attcgcagca gagccaagca atttgcattc aatcatctgt *F gaaggaaaag ctatcacaca aaatggcaca aatggacatc gaattgaaga aggccttcac *F cgagatgcag atcantgagc tggagacgac caagaagatc cacatgatcg acatgaagtg *F cgacgtggtc aagacaggca aacaaaagta ccagctgaca gaaaagggca cctgctgctt *F ggccgacgac acaagaattt accagtccgt gggtcgcatg ttcctgctta ccgatttaca *F gaatatgcgc gaggacctga aggctaggcn ggaaaaatgc gacaaagcga tngaactgct *F ggagaagaag aaggantctt gcagaagtcc ttaaaaacca ggaagacgtc tgcgctagtt *F ggtgcaccnc cgcagggaac ccatcagacn gcgaaataga ctaccctccg aactggcttt *F ntccctca *F Database: dmel_all_transcript_r320 *F >CG13993-RA type=mRNA; *F loc=2L:join(6061318..6061465,6061562..6061704, *F 6061774..6062055); ID=CG13993-RA; name=CG13993-RA; *F db_xref='CG13993,FlyBase:FBgn0031776'; len=573 *F Length = 573 *F Genome Map *F Score = 733 bits (381), Expect = 0.0 *F Identities = 464/494 (93%), Gaps = 6/494 (1%) *F Strand = Plus / Plus *F Query: 1 ggtgttgcaattttcagtgaattcgcagcagagccaagcaatttgcattcaatcatctgt 60 *F |||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| *F Sbjct: 37 ggtgttgcaattttcagtgaattcgcagcaaagccaagcaatttgcattcaatcatctgt 96 *F Query: 61 gaaggaaaagctatcacacaaaatggcacaaatggacatcgaattgaagaaggccttcac 120 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 97 gaaggaaaagctatcacacaaaatggcacaaatggacatcgaattgaagaaggccttcac 156 *F Query: 121 cgagatgcagatcantgagctggagacgaccaagaagatccacatgatcgacatgaagtg 180 *F |||||||||||||| | ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 157 cgagatgcagatcaataagctggagacgaccaagaagatccacatgatcgacatgaagtg 216 *F Query: 181 cgacgtggtcaagacaggcaaacaaaagtaccagctgacagaaaagggcacctgctgctt 240 *F |||| ||||||||||||||||||||||||||||||||||||||||||||||| || |||| *F Sbjct: 217 cgacatggtcaagacaggcaaacaaaagtaccagctgacagaaaagggcaccagcagctt 276 *F Query: 241 ggccgacgacacaagaatttaccagtccgtgggtcgcatgttcctgcttaccgatttaca 300 *F |||||||||||||||| |||||||||||||||||||||||||||||||||||||| |||| *F Sbjct: 277 ggccgacgacacaagagtttaccagtccgtgggtcgcatgttcctgcttaccgatgtaca 336 *F Query: 301 gaatatgcgcgaggacctgaaggctaggcnggaaaaatgcgacaaagcgatngaactgct 360 *F ||||||||||||||||||||||||||||| ||| ||||||||||||||||| |||||||| *F Sbjct: 337 gaatatgcgcgaggacctgaaggctaggcaggagaaatgcgacaaagcgatagaactgct 396 *F Query: 361 ggagaagaagaagga-ntcttgcagaagt-ccttaaaaaccaggaagac-gtctgcgcta 417 *F ||||||||||||||| |||||||||||| |||||| | |||||||||| |||||||| | *F Sbjct: 397 ggagaagaagaaggagttcttgcagaagtcccttaagagccaggaagacggtctgcgcga 456 *F Query: 418 gttggtgcaccnccgcagggaa-cccatcagacngcgaaatagactac-cctccgaactg 475 *F ||||||||| | |||| |||| || ||||||| |||||||||||||| |||| ||||| *F Sbjct: 457 gttggtgcagcagcgcaaggaagccgatcagactgcgaaatagactacaactccaaactg 516 *F Query: 476 gc-tttntccctca 488 *F || ||| ||||||| *F Sbjct: 517 gcatttatccctca 530 *F \# *F anon-EST:Posey289 = end of lace ? *F >anon-EST:Posey289 *F ggcacgagat tcactgtacg ggattcttaa gtctactagt gtaaatattt atttaaaata *F tataaaactt ggcgggcaga tgaatcttaa agtcgaaaaa tgttttgcct tgtattttac *F ataaattttg acacaactta tgcgacctct acagttaatt tacagaagtt ggatactcga *F cgcaaatata aaacacatat gtagagaatc cgctaagtct agggccttag ttcttatttt *F tgagaaaata agaagtacag tggccattaa ttaaataaag ctacttcttg aaccattttt *F aattaagtaa atgcctgact tttaagcgat aattagagat tagcgatatc aattaaatcg *F atattgataa tatgttattt tagacgattt ggaagtaaaa taaataaaat ttgtttcagt *F aaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003647|gb|AE003647|arm:2L 15243654..15505601 *F estimated-cyto:35C5-35D3 gadfly-seqname:AE003647 *F seq_release:3 *F Length = 261948 *F Score = 794 bits (413), Expect = 0.0 Genome Map *F Identities = 413/413 (100%) *F Strand = Plus / Plus *F Query: 9 attcactgtacgggattcttaagtctactagtgtaaatatttatttaaaatatataaaac 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239034 attcactgtacgggattcttaagtctactagtgtaaatatttatttaaaatatataaaac *F 239093 *F Query: 69 ttggcgggcagatgaatcttaaagtcgaaaaatgttttgccttgtattttacataaattt 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239094 ttggcgggcagatgaatcttaaagtcgaaaaatgttttgccttgtattttacataaattt *F 239153 *F Query: 129 tgacacaacttatgcgacctctacagttaatttacagaagttggatactcgacgcaaata 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239154 tgacacaacttatgcgacctctacagttaatttacagaagttggatactcgacgcaaata *F 239213 *F Query: 189 taaaacacatatgtagagaatccgctaagtctagggccttagttcttatttttgagaaaa 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239214 taaaacacatatgtagagaatccgctaagtctagggccttagttcttatttttgagaaaa *F 239273 *F Query: 249 taagaagtacagtggccattaattaaataaagctacttcttgaaccatttttaattaagt 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239274 taagaagtacagtggccattaattaaataaagctacttcttgaaccatttttaattaagt *F 239333 *F Query: 309 aaatgcctgacttttaagcgataattagagattagcgatatcaattaaatcgatattgat 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239334 aaatgcctgacttttaagcgataattagagattagcgatatcaattaaatcgatattgat *F 239393 *F Query: 369 aatatgttattttagacgatttggaagtaaaataaataaaatttgtttcagta 421 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 239394 aatatgttattttagacgatttggaagtaaaataaataaaatttgtttcagta 239446 *F Score = 33.4 bits (17), Expect = 4.7 Genome Map *F Identities = 17/17 (100%) *F Strand = Plus / Minus *F Query: 50 tatttaaaatatataaa 66 *F ||||||||||||||||| *F Sbjct: 138660 tatttaaaatatataaa 138644 *F Score = 33.4 bits (17), Expect = 4.7 Genome Map *F Identities = 21/23 (91%) *F Strand = Plus / Plus *F Query: 186 atataaaacacatatgtagagaa 208 *F ||||| | ||||||||||||||| *F Sbjct: 87488 atatataccacatatgtagagaa 87510 *F \# *F anon-EST:Posey292 = CG32172 *F >anon-EST:Posey292 *F ggcacgaggg gcacatcccg tctcatcccc actctctcga attgtgtccg ttggtagccg *F aagttacgat tgcaaaactt tcgtcatctc tctctcactc tctgaagcaa aagccaaggc *F cggagcaggt gcatatggtg ccaacccccc tggccccccc aaaagggggg tggtgctggt *F gcaattgcaa aggcacagga gcacaggagc agctaaataa acaagaaagc aaaatcaaca *F accgcaaaag tctaagacat gtatcacgaa ttgaaaatga taatgacaaa aa *F Database: dmel_all_transcript_r320 *F >CG32172-RA type=mRNA; loc=3L:complement(17348426..17350337); Genome Map *F ID=noe-RA; name=noe-RA; *F db_xref='CG32172,FlyBase:FBgn0026197'; len=1912 *F Length = 1912 *F Score = 417 bits (217), Expect = e-116 *F Identities = 250/283 (88%) *F Strand = Plus / Plus *F Query: 9 gggcacatcccgtctcatccccactctctcgaattgtgtccgttggtagccgaagttacg 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 143 gggcacatcccgtctcatccccactctctcgaattgtgtccgttggtagccgaagttacg 202 *F Query: 69 attgcaaaactttcgtcannnnnnnnnnnnnnnnngaagcaaaagccaaggccggagcag 128 *F |||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 203 attgcaaaactttcgtcatctctctctcactctctgaagcaaaagccaaggccggagcag 262 *F Query: 129 gtgcatatggtgccaannnnnnnnnnnnnnnnaaaaggggggtggtgctggtgcaattgc 188 *F |||||||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 263 gtgcatatggtgccaacccccctggcccccccaaaaggggggtggtgctggtgcaattgc 322 *F Query: 189 aaaggcacaggagcacaggagcagctaaataaacaagaaagcaaaatcaacaaccgcaaa 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 323 aaaggcacaggagcacaggagcagctaaataaacaagaaagcaaaatcaacaaccgcaaa 382 *F Query: 249 agtctaagacatgtatcacgaattgaaaatgataatgacaaaa 291 *F ||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 383 agtctaagacatgtatcacgaattgaaaatgataatgacaaaa 425 *F \# *F anon-EST:Posey294 = CG2848 *F >anon-EST:Posey294 *F ggcacgagtg aacctgatcc aggcctccgt ctttcagctg cactccaaca tgctggtgga *F tgtggccnac gtgctccacg aactgaaggc aggtggtggg caacgagcgg atgcagccct *F tcctggccca ggcgctggaa gcactgccca aaaagaacag cggtggctac ggtgacaccc *F acgcagcgac agtctggact aatttagcag cacanttctg agagcggaca ccacgaaagc *F catttcgcaa gccttgaaaa ccttcacgcg actctttcgc ttatcagggg agatgccatg *F agtttggatt cggatgattc tggtgccttt tggaatgatg tatgtcatgt gg *F Database: dmel_all_transcript_r320 *F >CG2848-SR-RA type=mRNA; *F loc=2L:join(2752484..2752715,2752786..2753877, *F 2754073..2754239,2754293..2755657,2755720..2756179); *F ID=Trn-SR-RA; name=Trn-SR-RA; *F db_xref='CG2848,FlyBase:FBgn0031456'; len=3316 *F Length = 3316 *F Genome Map *F Score = 477 bits (248), Expect = e-134 *F Identities = 323/344 (93%), Gaps = 7/344 (2%) *F Strand = Plus / Plus *F Query: 9 tgaacctgatccaggcctccgtctttcagctgcactccaacatgctggtggatgtggccn 68 *F |||||||||||||||| ||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 2646 tgaacctgatccaggcatccgtctttcagctgcactcctacatgctggtggatgtggccg 2705 *F Query: 69 acgtgctccacgaactgaaggcaggtggtgggcaacgagcggatgcagcccttcctggcc 128 *F | |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2706 aggtgctccacgaactgaag-caggtggtgggcaacgagcggatgcagcccttcctggcc 2764 *F Query: 129 caggcgctggaagcactgcccaaaaagaacagcggtggctacggtgacacccacgcagcg 188 *F ||||||||||| ||||||||||||||||||||||||||||||| ||||| ||||||||| *F Sbjct: 2765 caggcgctggaggcactgcccaaaaagaacagcggtggctacg-tgacagccacgcagca 2823 *F Query: 189 acagtctggactaatttagcagcacanttctgagagcggacaccacgaaagccatttcgc 248 *F |||| |||||| |||||||||||||| |||||||||||||||| |||||||||||||||| *F Sbjct: 2824 acag-ctggacgaatttagcagcacagttctgagagcggacacaacgaaagccatttcgc 2882 *F Query: 249 aagccttgaaaaccttcacgcgactctttcgcttatcaggggagatgccatgagtttgga 308 *F ||||||||||||||||||||||||||||||||| |||| |||||||| |||||||||||| *F Sbjct: 2883 aagccttgaaaaccttcacgcgactctttcgctaatca-gggagatg-catgagtttgga 2940 *F Query: 309 ttcggatgattctggtgccttttggaatgatgtatgtcatgtgg 352 *F ||||||||||||| || | |||| ||||||||||||| |||||| *F Sbjct: 2941 ttcggatgattct-gtacatttt-gaatgatgtatgtaatgtgg 2982 *F \# *F anon-EST:Posey35 = CG32172 *F >anon-EST:Posey35 *F cgcccgttcg tgtaatcgct ggcgcgccac gccccccgcc cctcccactg tcacgcgctc *F gccacgcccc ccagttgccg ccccattagc agcgggcaga tggacaacgt gtgggtggcc *F cacaaggaca tctagtaaca cgacgcccaa cagcagccgc aaggtctgat acgccgcccg *F ccacgcccat cgtgtttggg cggcagagga agcggtccgg aagcggaaac ggaaacgggc *F gagcaaaatg gtggcgcggt atcgcgggca aggcgacggc gcgtccacaa aaaataacca *F tagagacgtt taaggcaaat tctaaatgaa caaaagtata aaccaaaaac aattgtaacg *F agaaaacaaa cgaattcaaa aatgagcaat atgagcaaca actaataatg gcaaaaagca *F aaaacgacaa ctgcaaatta cgacacaaca ccttcgaaaa gacctcagta ataataaaaa *F Database: dmel_all_transcript_r320 *F >CG32172-RA type=mRNA; loc=3L:complement(17348426..17350337); Genome Map *F ID=noe-RA; name=noe-RA; *F db_xref='CG32172,FlyBase:FBgn0026197'; len=1912 *F Length = 1912 *F Score = 583 bits (303), Expect = e-166 *F Identities = 320/326 (98%), Gaps = 6/326 (1%) *F Strand = Plus / Plus *F Query: 3 cccgttcgtgtaatcgctggcgcgccacgccccccgcccctcccactgtcacgcgctcgc 62 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 634 cccgttcgtgtaatcgctggcgcgccacgccccccgcccctcccactgtcacgcgctcgc 693 *F Query: 63 cacgccccccagttgccgccccattagcagcgggcagatggacaacgtgtgggtggccca 122 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 694 cacgccccccagttgccgccccattagcagcgggcagatggacaacgtgtgggtggccca 753 *F Query: 123 caaggacatctagtaacacgacgcccaacagcagccgcaaggtctgatacgccgcccgcc 182 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 754 caaggacatctagtaacacgacgcccaacagcagccgcaaggtctgatacgccgcccgcc 813 *F Query: 183 acgcccatcgtgtttgggcggcagaggaagcggtccggaag------cggaaacggaaac 236 *F ||||||||||||||||||||||||||||||||||||||||| ||||||||||||| *F Sbjct: 814 acgcccatcgtgtttgggcggcagaggaagcggtccggaagcggaaacggaaacggaaac 873 *F Query: 237 gggcgagcaaaatggtggcgcggtatcgcgggcaaggcgacggcgcgtccacaaaaaata 296 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 874 gggcgagcaaaatggtggcgcggtatcgcgggcaaggcgacggcgcgtccacaaaaaata 933 *F Query: 297 accatagagacgtttaaggcaaattc 322 *F |||||||||||||||||||||||||| *F Sbjct: 934 accatagagacgtttaaggcaaattc 959 *F Score = 187 bits (97), Expect = 9e-47 *F Identities = 97/97 (100%) *F Strand = Plus / Plus *F Query: 384 gagcaatatgagcaacaactaataatggcaaaaagcaaaaacgacaactgcaaattacga 443 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1021 gagcaatatgagcaacaactaataatggcaaaaagcaaaaacgacaactgcaaattacga 1080 *F Query: 444 cacaacaccttcgaaaagacctcagtaataataaaaa 480 *F ||||||||||||||||||||||||||||||||||||| *F Sbjct: 1081 cacaacaccttcgaaaagacctcagtaataataaaaa 1117 *F \# *F anon-EST:Posey37 = CG9282 *F >anon-EST:Posey37 *F gagacgaatt cggcacgagg caagatgaaa attggcttgt gcgcattcag cgggtacaaa *F atctaccccg gtcatggcaa gaccatggtc aagatcgatg gcaattcgtt caccttcctg *F gacaaaaagt gcgagcgctc ctacctgatg aagcgcaatc cccgcaaggt tacttggacc *F gtgctgtacc gccggaagca ccgcaaggga atcgaggagg agcctccang gaagcgcacc *F cgccgcaccc agaatttcca gcgcgccatc gtcggtgcct cgctggccga nattctggcc *F aagcgtaaca tgaacccgag gtgcgcaagg cgcagcgcga ccaggccatc aaggtggcca *F aggaacaaaa gcgtgccgtc aaggccgcca aaaagctgct gcccccgctc ccgctaaaaa *F tcggccccaa ncagaagccg ccaaggtcac *F Database: dmel_all_transcript_r320 *F >CG9282-RA type=mRNA; *F loc=2L:complement(13382989..13383669,13383735..13383810, *F 13383926..13383985); ID=CG9282-RA; name=CG9282-RA; *F db_xref='CG9282,FlyBase:FBgn0032518'; len=817 *F Length = 817 *F Genome Map *F Score = 675 bits (351), Expect = 0.0 *F Identities = 415/436 (95%), Gaps = 5/436 (1%) *F Strand = Plus / Plus *F Query: 20 gcaagatgaaaattggcttgtgcgcattcagcgggtacaaaatctaccccggtcatggca 79 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 51 gcaagatgaaaattggcttgtgcgcattcagcgggtacaaaatctaccccggtcatggca 110 *F Query: 80 agaccatggtcaagatcgatggcaattcgttcaccttcctggacaaaaagtgcgagcgct 139 *F ||||||||||||||||||||||||| |||||||||||||||||||| ||||||||||||| *F Sbjct: 111 agaccatggtcaagatcgatggcaagtcgttcaccttcctggacaagaagtgcgagcgct 170 *F Query: 140 cctacctgatgaagcgcaatccccgcaaggttacttggaccgtgctgtaccgccggaagc 199 *F |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| *F Sbjct: 171 cctacctgatgaagcgcaatccccgcaaggttacgtggaccgtgctgtaccgccggaagc 230 *F Query: 200 accgcaagggaatcgaggaggagcctccanggaagcgcacccgccgcacccagaatttcc 259 *F ||||||||||||||||||||||| | | |||||||||||||||||||||||| |||| *F Sbjct: 231 accgcaagggaatcgaggaggaggcctccaagaagcgcacccgccgcacccagaagttcc 290 *F Query: 260 agcgcgccatcgtcggtgcctcgctggccganattctggccaagcgtaacatgaa-cccg 318 *F ||||||||||||||||||||||||||||||| ||||||||||||||||||||||| |||| *F Sbjct: 291 agcgcgccatcgtcggtgcctcgctggccgagattctggccaagcgtaacatgaagcccg 350 *F Query: 319 aggtgcgcaaggcgcagcgcgaccaggccatcaaggtggccaaggaacaaaagcgtgccg 378 *F |||||||||||||||||||||||||||||||||||||||||||||| || |||||||||| *F Sbjct: 351 aggtgcgcaaggcgcagcgcgaccaggccatcaaggtggccaaggagcagaagcgtgccg 410 *F Query: 379 tcaaggccgccaa-aaagctgctgcccccgctcccgctaa-aaatcggc-cccaancaga 435 *F ||||||||||||| || ||||||||||||||||||||||| || ||||| ||||| |||| *F Sbjct: 411 tcaaggccgccaagaaggctgctgcccccgctcccgctaagaagtcggcgcccaagcaga 470 *F Query: 436 a-gccgccaaggtcac 450 *F | |||||||||||||| *F Sbjct: 471 aggccgccaaggtcac 486 *F \# *F anon-EST:Posey5 = CG1263 *F >anon-EST:Posey5 *F ggcacgaggt accggtagga tccgtggtgg caagggcgac agcaaggaca agtaagctct *F ggctgcagca ggattccggc gtagcgcagg ttccgcctga gatctgggta gtggtcgtgc *F tctgctgtgc ggcgtcgtgg aagaaattgc tattctacat ggataatctg tatattctag *F gcatacgctt gacacggcaa aataaaacnt atttgtaaaa *F Database: dmel_all_transcript_r320 *F >CG1263-RB type=mRNA; *F loc=3L:join(2568108..2568142,2568205..2568519, *F 2568713..2569626); ID=RpL8-RB; name=RpL8-RB; *F db_xref='CG1263,FlyBase:FBgn0024939'; len=1264 *F Length = 1264 *F Genome Map *F Score = 387 bits (201), Expect = e-107 *F Identities = 208/212 (98%) *F Strand = Plus / Plus *F Query: 9 gtaccggtaggatccgtggtggcaagggcgacagcaaggacaagtaagctctggctgcag 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 937 gtaccggtaggatccgtggtggcaagggcgacagcaaggacaagtaagctctggctgcag 996 *F Query: 69 caggattccggcgtagcgcaggttccgcctgagatctgggtagtggtcgtgctctgctgt 128 *F |||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 997 caggattccggcgtggcgcaggttccgcctgagatctgggtagtggtcgtgctctgctgt 1056 *F Query: 129 gcggcgtcgtggaagaaattgctattctacatggataatctgtatattctaggcatacgc 188 *F ||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| *F Sbjct: 1057 gcggcgtcgtggaagaaattgctattctacatggataatctgtatgttctaggcatacgc 1116 *F Query: 189 ttgacacggcaaaataaaacntatttgtaaaa 220 *F ||||||||||| |||||||| ||||||||||| *F Sbjct: 1117 ttgacacggcagaataaaacgtatttgtaaaa 1148 *F \# *F anon-EST:Posey52 = CG31158? *F >anon-EST:Posey52 *F cacacgagca ttcatagata ctatatatag ttcatatata tatttatata tatgtgtata *F tgcatagtat aacggctttc ctaagtttaa tcaaagctta tcagttagta ataaatgttc *F acataaaaat caccgtctcc acgttcgtct tcttgtttcc cttctcgcca caagttggca *F agttgtctta aatgaattgt tatcgaaagg gtattgcttt agaaactaag tgatgccctc *F ttaagccagt taaaatgttt gactaaataa atatacaata tgcatacatt taggcgcttg *F gtttgcagca ggcaagttgc tttcactttt agttagtttg tattttcgaa tttattgccg *F tttcagtaga attgttaaaa attattataa acaatgttat atagtctgca tttacataat *F tacacaatac cataatcata atatattcaa aaatatatga tgcatttaat tttatttttt *F cctccatctc tctttacccc gtaatggata tttaatttca tattctagct acatggacat *F gtcgcgtaat caaccgatag ttgtttaaat aaatatatta atccgtacat aatattggag *F taactgttcc tacacagtta aatgtgataa tgtatcgcaa aagaccaaaa acaagttgta *F cacggtatgg tagtaattgc ttaagcttaa tgctaatgcg cttgtcctct tctcaattct *F catttcgtta ttgttactgc cgatgttctc catcttgtgg ttgtgtagag atattgttca *F ttaattaata atttatttty atcatttata atgatagcat aggtratgat gtaagtattg *F tttatcaact ctkgtaataa taagtctatg tgtataagca gttttcattc tcgttcctaa *F acagccgtcc agcagaatct ttggcggagc aaatatgaac gataattcaa attcgaatcg *F atctgactgt gtagctttaa atcaggtgaa accgatctcg aataccctca ttnctggata *F agtagcatgg taaatataaa atcaactgca cgaattcgag ataatcctct ggttgccgag *F atctcgtncc gaattcgtct c *F Database: dmel_all_transcript_r320 *F >CG31158-RB type=mRNA; *F loc=3R:join(18415336..18415350,18415597..18415663, *F 18415740..18415793,18416052..18416854,18416923..18418044, *F 18419223..18420301,18420368..18420754,18420808..18420971, *F 18421030..18421127,18421194..18421469,18421533..18421670, *F 18421731..18421948,18423575..18423686, *F 18423770..18424849); ID=CG31158-RB; name=CG31158-RB; *F db_xref='CG31158,FlyBase:FBgn0051158'; len=5613 *F Length = 5613 *F Genome Map *F Score = 1419 bits (737), Expect = 0.0 *F Identities = 744/748 (99%), Gaps = 1/748 (0%) *F Strand = Plus / Minus *F Query: 335 agtttgtattttcgaatttattgccgtttcagtagaattgttaaaaattattataaacaa 394 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5613 agtttgtattttcgaatttattgccgtttcagtagaattgttaaaaattattataaacaa 5554 *F Query: 395 tgttatatagtctgcatttacataattacacaataccataatcataatatattcaaaaat 454 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5553 tgttatatagtctgcatttacataattacacaataccataatcataatatattcaaaaat 5494 *F Query: 455 atatgatgcatttaattttattttttcctccatctctctttaccccgtaatggatattta 514 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5493 atatgatgcatttaattttattttttcctccatctctctttaccccgtaatggatattta 5434 *F Query: 515 atttcatattctagctacatggacatgtcgcgtaatcaaccgatagttgtttaaataaat 574 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5433 atttcatattctagctacatggacatgtcgcgtaatcaaccgatagttgtttaaataaat 5374 *F Query: 575 atattaatccgtacataatattggagtaactgttcctacacagttaaatgtgataatgta 634 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5373 atattaatccgtacataatattggagtaactgttcctacacagttaaatgtgataatgta 5314 *F Query: 635 tcgcaaaagaccaaaaacaagttgtacacggtatggtagtaattgcttaagcttaatgct 694 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5313 tcgcaaaagaccaaaaacaagttgtacacggtatggtagtaattgcttaagcttaatgct 5254 *F Query: 695 aatgcgcttgtcctcttctcaattctcatttcgttattgttactgccgatgttctccatc 754 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5253 aatgcgcttgtcctcttctcaattctcatttcgttattgttactgccgatgttctccatc 5194 *F Query: 755 ttgtggttgtgtagagatattgttcattaattaataatttattttyatcatttataatga 814 *F ||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| *F Sbjct: 5193 ttgtggttgtgtagagatattgttcattaattaataatttatttttatcatttataatga 5134 *F Query: 815 tagcataggtratgatgtaagtattgtttatcaactctkgtaataataagtctatgtgta 874 *F |||||||||| ||||||||||||||||||||||||||| ||||||||||||||||||||| *F Sbjct: 5133 tagcataggtaatgatgtaagtattgtttatcaactcttgtaataataagtctatgtgta 5074 *F Query: 875 taagcagttttcattctcgttcctaaacagccgtccagcagaatctttggcggagcaaat 934 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5073 taagcagttttcattctcgttcctaaacagccgtccagcagaatctttggcggagcaaat 5014 *F Query: 935 atgaacgataattcaaattcgaatcgatctgactgtgtagctttaaatcaggtgaaaccg 994 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 5013 atgaacgataattcaaattcgaatcgatctgactgtgtagctttaaatcaggtgaaaccg 4954 *F Query: 995 atctcgaataccctcattnctggataagtagcatggtaaatataaaatcaactgcacgaa 1054 *F |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 4953 atctcgaataccctcatt-ctggataagtagcatggtaaatataaaatcaactgcacgaa 4895 *F Query: 1055 ttcgagataatcctctggttgccgagat 1082 *F |||||||||||||||||||||||||||| *F Sbjct: 4894 ttcgagataatcctctggttgccgagat 4867 *F \# *F anon-EST:Posey55 = CG11739 *F >anon-EST:Posey55 *F ggcacgagtc gaaatactcc tgatattagc ctgaccgcat atgcttaccg ttcacagcaa *F gcgacacgtc agtgaatcaa atttacagtg tatattatag tgattgtgta aaagtgaaaa *F tgtatgaaac aaaaatatat ctgttaactg ttatctgaac tcagtttc *F Database: dmel_all_transcript_r320 *F >CG11739-RD type=mRNA; *F loc=3R:join(204643..204730,205171..205339,205396..205545, *F 205649..205766,205830..205991,206225..206352, *F 206424..206931); ID=CG11739-RD; name=CG11739-RD; *F db_xref='CG11739,FlyBase:FBgn0037239'; len=1323 *F Length = 1323 *F Genome Map *F Score = 308 bits (160), Expect = 1e-83 *F Identities = 160/160 (100%) *F Strand = Plus / Plus *F Query: 9 tcgaaatactcctgatattagcctgaccgcatatgcttaccgttcacagcaagcgacacg 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1164 tcgaaatactcctgatattagcctgaccgcatatgcttaccgttcacagcaagcgacacg 1223 *F Query: 69 tcagtgaatcaaatttacagtgtatattatagtgattgtgtaaaagtgaaaatgtatgaa 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1224 tcagtgaatcaaatttacagtgtatattatagtgattgtgtaaaagtgaaaatgtatgaa 1283 *F Query: 129 acaaaaatatatctgttaactgttatctgaactcagtttc 168 *F |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1284 acaaaaatatatctgttaactgttatctgaactcagtttc 1323 *F \# *F anon-EST:Posey59 = CG8472 *F >anon-EST:Posey59 *F acgagatcgt ttcgagcaac aagaacaaca agtctatcac tacacttaaa caaaatgagg *F agcgtgaaga agacgagcaa gcagagggag aagcaagaag cagcagcagc ntccaccatt *F attgccgaaa gatggcgccc atggcgcact aaggtcagcg cgccacctgg cgacggcggc *F tcgcaccggc tgcattttgt ttgacagttt tccactaagg aagtaaagaa aaaaaattat *F gaaaaccccc cactctgtaa gctagcaaat tgtgtttaaa gtataatnaa aaaattgtcg *F atttcgttgc gagaaagaat aaaantaaaa gct *F Database: dmel_all_transcript_r320 *F >CG8472-RB type=mRNA; *F loc=2R:join(7329273..7329388,7329456..7329630, *F 7333906..7334148,7338479..7339338); ID=Cam-RB; *F name=Cam-RB; db_xref='CG8472,FlyBase:FBgn0000253'; *F len=1394 *F Length = 1394 *F Genome Map *F Score = 533 bits (277), Expect = e-151 *F Identities = 312/330 (94%), Gaps = 3/330 (0%) *F Strand = Plus / Plus *F Query: 6 atcgtttcgagcaacaagaacaacaagtctatcactacacttaaacaaaatgaggagcgt 65 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1051 atcgtttcgagcaacaagaacaacaagtctatcactacacttaaacaaaatgaggagcga 1110 *F Query: 66 gaagaagacgagcaagcagagggagaagcaagaagcagcagcagcntccaccattattgc 125 *F ||||||||||||||||||||||||||||||||||||||||||||| ||||||| |||||| *F Sbjct: 1111 gaagaagacgagcaagcagagggagaagcaagaagcagcagcagcatccaccagtattgc 1170 *F Query: 126 cgaaagatggcgcccatggcgcactaaggtcagcgcgccacctggcgacggcggctcgca 185 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1171 cgaaagatggcgcccatggcgcactaaggtcagcgcgccacctggcgacggcggctcgca 1230 *F Query: 186 ccggctgcattttgtttgacagttttccactaaggaagt--aaagnnnnnnnnttatgaa 243 *F ||||||||||||||||||||||||||||||||| ||||| ||| | ||||| *F Sbjct: 1231 ccggctgcattttgtttgacagttttccactaa-gaagtaaaaaaaaagaaaataatgaa 1289 *F Query: 244 aaccccccactctgtaagctagcaaattgtgtttaaagtataatnaaaaaattgtcgatt 303 *F |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| *F Sbjct: 1290 aaccccccactctgtaagctagcaaattgtgtttaaagtataatgaaaaaattgtcgatt 1349 *F Query: 304 tcgttgcgagaaagaataaaantaaaagct 333 *F ||||||||||||||||||||| |||||||| *F Sbjct: 1350 tcgttgcgagaaagaataaaaataaaagct 1379 *F \# *F anon-EST:Posey6 = CG6253 *F >anon-EST:Posey6 *F ggcacgagtt tttttttncc taaaaataac actttctttt atttcctcca tggtggtgga *F tnttcatcaa gcagttactt tcgttaaagg tagtattttt tacttcttgg ccgcggcctt *F cttgccaccc ttggccttct cggcacncaa accctcgcaa caatccttct tgaggacccg *F gggattnccn tcagccttgg tgcnctnctt cattttttta aaggcaatgg tcagcacctt *F nttgcnctaa cccttggcat aacccacctt gaagcggtca aattctttca acaaggagcn *F cttgcaaatt ttctntgcct tgacggacca ggggctnacc ttccnctggg ccttcaggtc *F gctctctntc caggccttgc ccacaatc *F Database: dmel_all_transcript_r320 *F >CG6253-RA type=mRNA; *F loc=3L:join(8559712..8559747,8560024..8560125, *F 8560358..8560552,8560813..8561109); ID=RpL14-RA; *F name=RpL14-RA; db_xref='CG6253,FlyBase:FBgn0017579'; *F len=630 *F Length = 630 *F Genome Map *F Score = 512 bits (266), Expect = e-144 *F Identities = 326/366 (89%) *F Strand = Plus / Minus *F Query: 19 cctaaaaataacactttcttttatttcctccatggtggtggatnttcatcaagcagttac 78 *F |||||||||||||||||||||||||| |||||||||||||||| |||||||||||||||| *F Sbjct: 615 cctaaaaataacactttcttttattttctccatggtggtggatgttcatcaagcagttac 556 *F Query: 79 tttcgttaaaggtagtattttttacttcttggccgcggccttcttgccacccttggcctt 138 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 555 tttcgttaaaggtagtattttttacttcttggccgcggccttcttgccacccttggcctt 496 *F Query: 139 ctcggcacncaaaccctcgcaacaatccttcttgaggacccggggattnccntcagcctt 198 *F |||||||| || || ||||| || ||||||||||||||| |||||| | || |||||||| *F Sbjct: 495 ctcggcacgcagacgctcgcgacgatccttcttgaggacgcggggagtgccgtcagcctt 436 *F Query: 199 ggtgcnctncttcannnnnnnaaaggcaatggtcagcaccttnttgcnctaacccttggc 258 *F ||||| || ||||| ||||| |||||||||| ||| |||| || || |||||| *F Sbjct: 435 ggtgcgcttcttcagtgtgttgaaggcgatggtcagcagcttgttgcgctgacgcttggc 376 *F Query: 259 ataacccaccttgaagcggtcaaattctttcaacaaggagcncttgcaaattttctntgc 318 *F ||||| || ||||||||||||||| || |||||| |||||| |||||| || |||| ||| *F Sbjct: 375 ataacgcagcttgaagcggtcaaagtcgttcaacgaggagcgcttgcagatgttctgtgc 316 *F Query: 319 cttgacggaccaggggctnaccttccnctgggccttcaggtcgctctctntccaggcctt 378 *F |||||||||||||||||| ||||||| |||||||||||||||||||||| |||||||||| *F Sbjct: 315 cttgacggaccaggggctgaccttccactgggccttcaggtcgctctctgtccaggcctt 256 *F Query: 379 gcccac 384 *F || ||| *F Sbjct: 255 gcgcac 250 *F \# *F anon-EST:Posey64 = CG11593 *F >anon-EST:Posey64 *F ggcacgagat tttatagagg aacatacata tgcaaccaga aaccaccaaa cttccgaaca *F tgttaccttt cctttgtagt ggcaaaaaga aaaaaaaaaa acacaaagtt ggaaacggag *F caacacacta aactgaatct aaacataagt aattgaaaat ctttagctga attttgcatc *F ttatatcgca tattgtatat tccaagcgtt ccaaacacga cactttcgct gacacatgat *F ggaccccatg ccagtcgaca gtaggtgccc catttatctc atccaccatc agagttagcc *F gattagcctg taagtgaaat agcgaccatt ctttgtggaa tctacacacg gacacacgta *F gaatctctct agcggataat gtgacgtaca attaggcaat gtaaccgatt gatattaaga *F gcgacatttt gtggtttcct tttaccgctt taaacaaaca ataataataa agtgcaagat *F gatttttatt tattacgcaa taaaaa *F Database: dmel_all_transcript_r320 *F >CG11593-RA type=mRNA; *F loc=3L:complement(4018216..4019208,4019271..4019381, *F 4019443..4020660,4020725..4021111); ID=CG11593-RA; *F name=CG11593-RA; db_xref='CG11593,FlyBase:FBgn0035488'; *F len=2709 *F Length = 2709 *F Genome Map *F Score = 819 bits (426), Expect = 0.0 *F Identities = 451/470 (95%), Gaps = 1/470 (0%) *F Strand = Plus / Plus *F Query: 9 attttatagaggaacatacatatgcaaccagaaaccaccaaacttccgaacatgttacct 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2240 attttatagaggaacatacatatgcaaccagaaaccaccaaacttccgaacatgttacct 2299 *F Query: 69 ttcctttgtagtggcnnnnnnnnnnnnnnnnnncacaaagttggaaacggagcaacacac 128 *F ||||||||||||||| ||||||||||||||||||||||||||| *F Sbjct: 2300 ttcctttgtagtggcaaaaagaaaaaaaaaaaacacaaagttggaaacggagcaacacac 2359 *F Query: 129 taaactgaatctaaacataagtaattgaaaatctttagctgaattttgcatcttatatcg 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2360 taaactgaatctaaacataagtaattgaaaatctttagctgaattttgcatcttatatcg 2419 *F Query: 189 catattgtatattccaagcgttccaaacacgacactttcgctgacacatgatggacccca 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2420 catattgtatattccaagcgttccaaacacgacactttcgctgacacatgatggacccca 2479 *F Query: 249 tgccagtcgacagtaggtgccccatttatctcatccaccatcagagttagccgattagcc 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2480 tgccagtcgacagtaggtgccccatttatctcatccaccatcagagttagccgattagcc 2539 *F Query: 309 tgtaagtgaaatagcgaccattctttgtggaatctacacacggacacacgtagaatctct 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2540 tgtaagtgaaatagcgaccattctttgtggaatctacacacggacacacgtagaatctct 2599 *F Query: 369 ctagcggataatgtgacgtacaattaggcaatgtaaccgattgatattaagagcgacatt 428 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2600 ctagcggataatgtgacgtacaattaggcaatgtaaccgattgatattaagagcgacatt 2659 *F Query: 429 ttg-tggtttccttttaccgctttaaacaaacaataataataaagtgcaa 477 *F ||| |||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2660 ttgttggtttccttttaccgctttaaacaaacaataataataaagtgcaa 2709 *F \# *F anon-EST:Posey65 = CG16982 *F >anon-EST:Posey65 *F ggcacgagag cgctttgagg tgaagaagtg gaacgccgtg gctctgtggg cctgggacat *F cgtggtggac aactgcgcca tctgccgcaa ccacatcatg gacttgtgca tcgagtgtca *F ggcgaaccag gcgtccgcca ctagcgagga gtgcaccgtg gcctggggcg tctgcaacca *F cgccttccat ttccactgca tctctcgctg gctaaagacg cgccaggtat gcccactgga *F caaccgcgag tgggatttcc agaagtacgg ccactaaaat ctacaaaacc cccaagaccc *F accagatgga gggagcagca cttcccgcaa gctagatgat ggaggcctgc ttcatctgga *F catttgaata cctttttaga tcttaagcta gtggggcgat tcgagtgaac tggtagcctt *F taggagcaca gttaccgatt tccgagactt ccccgggaag cgcacctttt tgtacgcgtt *F aaataaaagc tgtagaagat ggagaaagac tnaaaaa *F Database: dmel_all_transcript_r320 *F >CG16982-RA type=mRNA; *F loc=X:join(404724..404962,405047..405074, *F 405140..406040); ID=Roc1a-RA; name=Roc1a-RA; *F db_xref='CG16982,FlyBase:FBgn0025638'; len=1168 *F Length = 1168 *F Genome Map *F Score = 929 bits (483), Expect = 0.0 *F Identities = 497/499 (99%), Gaps = 2/499 (0%) *F Strand = Plus / Plus *F Query: 9 agcgctttgaggtgaagaagtggaacgccgtggctctgtgggcctgggacatcgtggtgg 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 220 agcgctttgaggtgaagaagtggaacgccgtggctctgtgggcctgggacatcgtggtgg 279 *F Query: 69 acaactgcgccatctgccgcaaccacatcatggacttgtgcatcgagtgtcaggcgaacc 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 280 acaactgcgccatctgccgcaaccacatcatggacttgtgcatcgagtgtcaggcgaacc 339 *F Query: 129 aggcgtccgccactagcgaggagtgcaccgtggcctggggcgtctgcaaccacgccttcc 188 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 340 aggcgtccgccactagcgaggagtgcaccgtggcctggggcgtctgcaaccacgccttcc 399 *F Query: 189 atttccactgcatctctcgctggctaaagacgcgccaggtatgcccactggacaaccgcg 248 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 400 atttccactgcatctctcgctggctaaagacgcgccaggtatgcccactggacaaccgcg 459 *F Query: 249 agtgggatttccagaagtacggccactaaaatctacaaaacccccaagacccaccagatg 308 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 460 agtgggatttccagaagtacggccactaaaatctacaaaacccccaagacccaccagatg 519 *F Query: 309 gagggagcagcacttcccgcaagctagatgatggaggcctgcttcatctggacatttgaa 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 520 gagggagcagcacttcccgcaagctagatgatggaggcctgcttcatctggacatttgaa 579 *F Query: 369 tacctttttagatcttaagctagtggggcgattcgagtgaactggtagcctttaggagca 428 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 580 tacctttttagatcttaagctagtggggcgattcgagtgaactggtagcctttaggagca 639 *F Query: 429 cagttaccgatttccgagacttccccgggaagcgcacctttttgt-acgcgttaaataaa 487 *F |||||||||||||||||||| |||||||||||||||||||||||| |||||||||||||| *F Sbjct: 640 cagttaccgatttccgagac-tccccgggaagcgcacctttttgtaacgcgttaaataaa 698 *F Query: 488 agctgtagaagatggagaa 506 *F ||||||||||||||||||| *F Sbjct: 699 agctgtagaagatggagaa 717 *F \# *F anon-EST:Posey67 = CG8309 *F >anon-EST:Posey67 *F gcacgagaag cgccgaagac gtgtgtgcgt gttattttca gctgaatttg tttaaaaaag *F tggaataaca tttaacaaaa ccatgacttc gcatccggtt ttcatagacc tgtccctgga *F cgagcaggtg caagagctgc gcaagtattt caagaagctg ggagccgaaa tctcatcgga *F gaagtccaat aaaggtgtgg aggatgattt gcacaagatc atcggcgtct gcgacgtttg *F ctttaaggat ggtgagccct cgcagattga tggcattctg aacagcattg tgtccatcat *F gatcacgata cccctggatc gcggtgagaa cattgtcctg gcctactgcg agaagatgac *F caaggctccc aatcttccat tgggcaaagg tgtgccttnc agtcgttgtg gcgtctgttc *F aacnaacctg gacaccgcct ctcctttacg ctaccatgtg tactaccacc tggtccaggt *F ggccaagcag tgcgaacagg tgctggaggt cttctcaggt gtggatcagc tcaaatccca *F gtttgccaac tgcccacctt cgtcggaaca gatgcagaag ctgtaccgcc tgctgcacga *F cgtgaccaag gacaccaacc tggagctgtc ttccaaggtt atgattgagc tgctgggcac *F ctacacggcg gacaatgctt gtgttgcccg tgaggatgcc atgaagtgca ttgtgactgc *F cttggctgac cccaatacat tcctgctgga tcctctgctg tcgctgaagc ctgtgcgctt *F tttggaaggc gacctcatcc acgacctgct gtccatcttc gtgtccgaga agctgccagc *F gtacgtgcag ttctacgagg atcacaggga gtttgtcaac tcgcaaggat tgaaccatga *F gcagaacatg aagnaagatg cgtctgctga ccttcatgca gctggccgag agcagcccgg *F agatgacatt cgaaancgct taccaaggag ctgcagatca acgaagacga ggtggagccc *F ttcgtcattg aggtgctgaa aacaaagctg gtacgcgcac gactagatca ggccaatcaa *F aaggtacaca tctcatcgac aatgcaccga acctttggag caccacaatg ggagcagctt *F cgcgatttgc tgcaggcatg gaaggagaac ctcagcacag tgcgcgaggg tctaacgagc *F gtctcctcgg cgcaactgga tctggctcga tcccagaagc tgatacacta ggcgcaccac *F ttccaaaaaa attgcactga tgatgaacaa atgctggcct cggggcactg gagtgacgac *F ctaaattatt ttcaagaatg cgagagggct ggataacttg aataaattta taagtaaaat *F ctgtaaaaag cccggg *F Database: dmel_all_transcript_r320 *F >CG8309-RA type=mRNA; *F loc=2R:complement(9227837..9228907,9228962..9229141, *F 9229258..9229640); ID=CG8309-RA; name=CG8309-RA; *F db_xref='CG8309,FlyBase:FBgn0033902'; len=1634 *F Length = 1634 *F Genome Map *F Score = 2498 bits (1299), Expect = 0.0 *F Identities = 1357/1374 (98%), Gaps = 6/1374 (0%) *F Strand = Plus / Plus *F Query: 8 aagcgccgaagacgtgtgtgcgtgttattttcagctgaatttgtttaaaaaagtggaata 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 264 aagcgccgaagacgtgtgtgcgtgttattttcagctgaatttgtttaaaaaagtggaata 323 *F Query: 68 acatttaacaaaaccatgacttcgcatccggttttcatagacctgtccctggacgagcag 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 324 acatttaacaaaaccatgacttcgcatccggttttcatagacctgtccctggacgagcag 383 *F Query: 128 gtgcaagagctgcgcaagtatttcaagaagctgggagccgaaatctcatcggagaagtcc 187 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 384 gtgcaagagctgcgcaagtatttcaagaagctgggagccgaaatctcatcggagaagtcc 443 *F Query: 188 aataaaggtgtggaggatgatttgcacaagatcatcggcgtctgcgacgtttgctttaag 247 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 444 aataaaggtgtggaggatgatttgcacaagatcatcggcgtctgcgacgtttgctttaag 503 *F Query: 248 gatggtgagccctcgcagattgatggcattctgaacagcattgtgtccatcatgatcacg 307 *F |||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||||| *F Sbjct: 504 gatggtgagccctcgcagattgatggaattctaaacagcattgtgtccatcatgatcacg 563 *F Query: 308 atacccctggatcgcggtgagaacattgtcctggcctactgcgagaagatgaccaaggct 367 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 564 atacccctggatcgcggtgagaacattgtcctggcctactgcgagaagatgaccaaggct 623 *F Query: 368 cccaatcttccattgggcaaaggtgtgccttncagtcgttgtggcgtctgttcaacnaac 427 *F |||||||||||||||||||| |||||||||| |||||||||||||||||||||||| ||| *F Sbjct: 624 cccaatcttccattgggcaa-ggtgtgcctt-cagtcgttgtggcgtctgttcaac-aac 680 *F Query: 428 ctggacaccgcctctcctttacgctaccatgtgtactaccacctggtccaggtggccaag 487 *F ||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 681 ctggacaccgcctctcccttacgctaccatgtgtactaccacctggtccaggtggccaag 740 *F Query: 488 cagtgcgaacaggtgctggaggtcttctcaggtgtggatcagctcaaatcccagtttgcc 547 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 741 cagtgcgaacaggtgctggaggtcttctcaggtgtggatcagctcaaatcccagtttgcc 800 *F Query: 548 aactgcccaccttcgtcggaacagatgcagaagctgtaccgcctgctgcacgacgtgacc 607 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 801 aactgcccaccttcgtcggaacagatgcagaagctgtaccgcctgctgcacgacgtgacc 860 *F Query: 608 aaggacaccaacctggagctgtcttccaaggttatgattgagctgctgggcacctacacg 667 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 861 aaggacaccaacctggagctgtcttccaaggttatgattgagctgctgggcacctacacg 920 *F Query: 668 gcggacaatgcttgtgttgcccgtgaggatgccatgaagtgcattgtgactgccttggct 727 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 921 gcggacaatgcttgtgttgcccgtgaggatgccatgaagtgcattgtgactgccttggcc 980 *F Query: 728 gaccccaatacattcctgctggatcctctgctgtcgctgaagcctgtgcgctttttggaa 787 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 981 gaccccaatacattcctgctggatcctctgctgtcgctgaagcctgtgcgctttttggaa 1040 *F Query: 788 ggcgacctcatccacgacctgctgtccatcttcgtgtccgagaagctgccagcgtacgtg 847 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1041 ggcgacctcatccacgacctgctgtccatcttcgtgtccgagaagctgccagcgtacgtg 1100 *F Query: 848 cagttctacgaggatcacagggagtttgtcaactcgcaaggattgaaccatgagcagaac 907 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1101 cagttctacgaggatcacagggagtttgtcaactcgcaaggattgaaccatgagcagaac 1160 *F Query: 908 atgaagnaagatgcgtctgctgaccttcatgcagctggccgagagcagcccggagatgac 967 *F |||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1161 atgaag-aagatgcgtctgctgaccttcatgcagctggccgagagcagcccggagatgac 1219 *F Query: 968 attcgaaancgcttaccaaggagctgcagatcaacgaagacgaggtggagcccttcgtca 1027 *F |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1220 attcgaaa-cgcttaccaaggagctgcagatcaacgaagacgaggtggagcccttcgtca 1278 *F Query: 1028 ttgaggtgctgaaaacaaagctggtacgcgcacgactagatcaggccaatcaaaaggtac 1087 *F | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1279 tcgaggtgctgaaaacaaagctggtacgcgcacgactagatcaggccaatcaaaaggtac 1338 *F Query: 1088 acatctcatcgacaatgcaccgaacctttggagcaccacaatgggagcagcttcgcgatt 1147 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1339 acatctcatcgacaatgcaccgaacctttggagcaccacaatgggagcagcttcgcgatt 1398 *F Query: 1148 tgctgcaggcatggaaggagaacctcagcacagtgcgcgagggtctaacgagcgtctcct 1207 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1399 tgctgcaggcatggaaggagaacctcagcacagtgcgcgagggtctaacgagcgtctcct 1458 *F Query: 1208 cggcgcaactggatctggctcgatcccagaagctgatacactaggcgcaccacttccnnn 1267 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1459 cggcgcaactggatctggctcgatcccagaagctgatacactaggcgcaccacttcc-aa 1517 *F Query: 1268 nnnnttgcactgatgatgaacaaatgctggcctcggggcactggagtgacgacctaaatt 1327 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1518 aaaattgcactgatgatgaacaaatgctggcctcggggcactggagtgacgacctaaatt 1577 *F Query: 1328 attttcaagaatgcgagagggctggataacttgaataaatttataagtaaaatc 1381 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1578 attttcaagaatgcgagagggctggataacttgaataaatttataagtaaaatc 1631 *F \# *F anon-EST:Posey69 = CG31543 *F >anon-EST:Posey69 *F acccaccaaa cccaaaaaaa gagacgaatt cggcacgagg ccgacagcgc agctaaaggc *F aaccaaaaag tgtaaattat tttcaaccaa acacacatgt ataaagctag ttaaaaacta *F tttatagctt cggaggggcg gcagcgcaag cccgcattgc gaaagttaat caaagctcct *F ttagtcgtta agccttctag tttagtctct aagtcgtacc cttagtcatt ttcgcattaa *F ccattagctt actgccatgt cagcgtccga gttggttgtt tattaatttt agtttgttgc *F atctttgtca ggaccttttg cctagctcat tcttagtttt tggctgccaa agtattatac *F ctaaagagaa gttaactaga ttcaataaca taagcaactg tcgcgacgct cattgcatct *F tatctcaaaa ttatttaaca agccagtaaa tcgtggacaa acgcggtcac tggctaacac *F tcaatctgtc gactctcatg catcttgtta gacatctttt tcatatcgtt tacatctaat *F aacaaacgga aataaatgtt ngtccanata cgttctcttg ttatctgtaa atcatgaagt *F atgtatattt atgacatatc tacatattgt atgtatattt tttatattaa acaaaagcct *F gagctgatga accacgtgtg gccnacattt gtctacgtgg ttgtagagag atggatttta *F taagtg *F Database: dmel_all_transcript_r320 *F >CG31543-RB type=mRNA; *F loc=3R:join(1090663..1090921,1092472..1092751, *F 1092840..1092920,1092982..1093121,1093181..1094194); *F ID=Hph-RB; name=Hph-RB; *F db_xref='CG31543,FlyBase:FBgn0037308'; len=1774 *F Length = 1774 *F Genome Map *F Score = 1204 bits (626), Expect = 0.0 *F Identities = 628/630 (99%) *F Strand = Plus / Plus *F Query: 40 gccgacagcgcagctaaaggcaaccaaaaagtgtaaattattttcaaccaaacacacatg 99 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1145 gccgacagcgcagctaaaggcaaccaaaaagtgtaaattattttcaaccaaacacacatg 1204 *F Query: 100 tataaagctagttaaaaactatttatagcttcggaggggcggcagcgcaagcccgcattg 159 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1205 tataaagctagttaaaaactatttatagcttcggaggggcggcagcgcaagcccgcattg 1264 *F Query: 160 cgaaagttaatcaaagctcctttagtcgttaagccttctagtttagtctctaagtcgtac 219 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1265 cgaaagttaatcaaagctcctttagtcgttaagccttctagtttagtctctaagtcgtac 1324 *F Query: 220 ccttagtcattttcgcattaaccattagcttactgccatgtcagcgtccgagttggttgt 279 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1325 ccttagtcattttcgcattaaccattagcttactgccatgtcagcgtccgagttggttgt 1384 *F Query: 280 ttattaattttagtttgttgcatctttgtcaggaccttttgcctagctcattcttagttt 339 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1385 ttattaattttagtttgttgcatctttgtcaggaccttttgcctagctcattcttagttt 1444 *F Query: 340 ttggctgccaaagtattatacctaaagagaagttaactagattcaataacataagcaact 399 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1445 ttggctgccaaagtattatacctaaagagaagttaactagattcaataacataagcaact 1504 *F Query: 400 gtcgcgacgctcattgcatcttatctcaaaattatttaacaagccagtaaatcgtggaca 459 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1505 gtcgcgacgctcattgcatcttatctcaaaattatttaacaagccagtaaatcgtggaca 1564 *F Query: 460 aacgcggtcactggctaacactcaatctgtcgactctcatgcatcttgttagacatcttt 519 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1565 aacgcggtcactggctaacactcaatctgtcgactctcatgcatcttgttagacatcttt 1624 *F Query: 520 ttcatatcgtttacatctaataacaaacggaaataaatgttngtccanatacgttctctt 579 *F ||||||||||||||||||||||||||||||||||||||||| ||||| |||||||||||| *F Sbjct: 1625 ttcatatcgtttacatctaataacaaacggaaataaatgtttgtccaaatacgttctctt 1684 *F Query: 580 gttatctgtaaatcatgaagtatgtatatttatgacatatctacatattgtatgtatatt 639 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1685 gttatctgtaaatcatgaagtatgtatatttatgacatatctacatattgtatgtatatt 1744 *F Query: 640 ttttatattaaacaaaagcctgagctgatg 669 *F |||||||||||||||||||||||||||||| *F Sbjct: 1745 ttttatattaaacaaaagcctgagctgatg 1774 *F \# *F anon-EST:Posey7 = end of CHES-1-like ? *F >anon-EST:Posey7 *F ggcacgaggt tacacgtaca tagttgaaat atgctaaaca tttgttaaca cgaatccaat *F cgtaagctag ccgattttag ttgaatgaac ctcctgcaat tttcctaccc aaggtgtaca *F tatatacaaa atggattaag ctgttgaaat gatgctgcac atctttacat aggcatattg *F tggaaaccaa gaaatactta ttaattaaat aattaaatag gcgaaaggaa aaaaacttga *F caaagaacgc cctaagtgtt tacacttcat gtaccttttt agcaaaaagc aaaaaacgca *F aaagcaaaga tcgcattaaa attgtataca tacattatat ttaaactacc aattacgaaa *F ctaaactaaa tgttgatgtc aaagagaaac caatttgcta gttgttacca atcagaaaag *F aacactttaa acaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003441|gb|AE003441|arm:X 7253939..7539888 *F estimated-cyto:7B3-7B8 gadfly-seqname:AE003441 *F seq_release:3 *F Length = 285950 *F Score = 498 bits (259), Expect = e-140 Genome Map *F Identities = 266/273 (97%) *F Strand = Plus / Minus *F Query: 8 ggttacacgtacatagttgaaatatgctaaacatttgttaacacgaatccaatcgtaagc 67 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 171016 ggttacacgtacatagttgaaatatgctaaacatttgttaacacgaatccaatcgtaagc *F 170957 *F Query: 68 tagccgattttagttgaatgaacctcctgcaattttcctacccaaggtgtacatatatac 127 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 170956 tagccgattttagttgaatgaacctcctgcaattttcctacccaaggtgtacatatatac *F 170897 *F Query: 128 aaaatggattaagctgttgaaatgatgctgcacatctttacataggcatattgtggaaac 187 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 170896 aaaatggattaagctgttgaaatgatgctgcacatctttacataggcatattgtggaaac *F 170837 *F Query: 188 caagaaatacttattaattaaataattaaataggcgaaaggnnnnnnncttgacaaagaa 247 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||||| *F Sbjct: 170836 caagaaatacttattaattaaataattaaataggcgaaaggaaaaaaacttgacaaagaa *F 170777 *F Query: 248 cgccctaagtgtttacacttcatgtaccttttt 280 *F ||||||||||||||||||||||||||||||||| *F Sbjct: 170776 cgccctaagtgtttacacttcatgtaccttttt 170744 *F Score = 237 bits (123), Expect = 2e-61 Genome Map *F Identities = 127/129 (98%) *F Strand = Plus / Minus *F Query: 309 gatcgcattaaaattgtatacatacattatatttaaactaccaattacgaaactaaacta 368 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 170715 gatcgcattaaaattgtatacatacattatatttaaactaccaattacgaaactaaacta *F 170656 *F Query: 369 aatgttgatgtcaaagagaaaccaatttgctagttgttaccaatcagaaaagaacacttt 428 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | *F Sbjct: 170655 aatgttgatgtcaaagagaaaccaatttgctagttgttaccaatcagaaaagaacacata *F 170596 *F Query: 429 aaacaaaaa 437 *F ||||||||| *F Sbjct: 170595 aaacaaaaa 170587 *F Score = 33.4 bits (17), Expect = 4.8 Genome Map *F Identities = 19/20 (95%) *F Strand = Plus / Plus *F Query: 159 acatctttacataggcatat 178 *F ||||||||||||| |||||| *F Sbjct: 6725 acatctttacatacgcatat 6744 *F \# *F anon-EST:Posey79 = CG5889 *F >anon-EST:Posey79 *F ggcacgagat cgattacccc ctacaaaccg accgcctaga acaattggct agacagcaga *F gctaactggt gagaacaacc caaacaagca aacaaccaaa caactaaaca gccaaccatc *F caaccgattc cacatgtaaa acaacttaac tcgctgccgt taaccaccac caactatcta *F agttagaaca ttatgctttc tatcgattag ttcacttata tctgtagata tattcgagcg *F acgattatgt ttgttgttta gccatatctg atatttatta acgcaattaa gcagctttgt *F tataatttat tgttaaaagg tcgagcctaa tgtatcagta actagaaaac ctaacccctt *F atgatcgatc ggaccgattc agccattgta ccactgacta gatgatgatg atgtgcgata *F aacaataaaa cgaaaacaca agttaaaaa *F Database: dmel_all_transcript_r320 *F >CG5889-RA type=mRNA; *F loc=3R:join(22966728..22966993,22969303..22969477, *F 22969543..22970592,22970653..22970805,22970865..22971159, *F 22971228..22971356,22971427..22972693); ID=Mdh-RA; *F name=Mdh-RA; db_xref='CG5889,FlyBase:FBgn0029155'; *F len=3335 *F Length = 3335 *F Genome Map *F Score = 621 bits (323), Expect = e-177 *F Identities = 323/323 (100%) *F Strand = Plus / Plus *F Query: 125 cgattccacatgtaaaacaacttaactcgctgccgttaaccaccaccaactatctaagtt 184 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3013 cgattccacatgtaaaacaacttaactcgctgccgttaaccaccaccaactatctaagtt 3072 *F Query: 185 agaacattatgctttctatcgattagttcacttatatctgtagatatattcgagcgacga 244 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3073 agaacattatgctttctatcgattagttcacttatatctgtagatatattcgagcgacga 3132 *F Query: 245 ttatgtttgttgtttagccatatctgatatttattaacgcaattaagcagctttgttata 304 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3133 ttatgtttgttgtttagccatatctgatatttattaacgcaattaagcagctttgttata 3192 *F Query: 305 atttattgttaaaaggtcgagcctaatgtatcagtaactagaaaacctaaccccttatga 364 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3193 atttattgttaaaaggtcgagcctaatgtatcagtaactagaaaacctaaccccttatga 3252 *F Query: 365 tcgatcggaccgattcagccattgtaccactgactagatgatgatgatgtgcgataaaca 424 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3253 tcgatcggaccgattcagccattgtaccactgactagatgatgatgatgtgcgataaaca 3312 *F Query: 425 ataaaacgaaaacacaagttaaa 447 *F ||||||||||||||||||||||| *F Sbjct: 3313 ataaaacgaaaacacaagttaaa 3335 *F Score = 112 bits (58), Expect = 3e-24 *F Identities = 65/66 (98%), Gaps = 1/66 (1%) *F Strand = Plus / Plus *F Query: 9 atcgattaccccc-tacaaaccgaccgcctagaacaattggctagacagcagagctaact 67 *F ||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 2896 atcgattacccccctacaaaccgaccgcctagaacaattggctagacagcagagctaact 2955 *F Query: 68 ggtgag 73 *F |||||| *F Sbjct: 2956 ggtgag 2961 *F \# *F anon-EST:Posey83 = ? *F >anon-EST:Posey83 *F gcgcgaggtn cccaacgcct tttcctcatt tggcgcacca caaaagcgga aagcgaccac *F agagccccaa gcacactgag attgccggag acaaccanca gccaagtctg aagccaaaat *F cgaatcgaag tcctaancga atatcaaatc caagcccaaa accaaancct actattaaca *F aatagccnat agcgaggagt tcacgaagga gtcctgcaaa cacatccttt caacacagtg *F caagctcgcc attttgtgtt ttttcgaaat agaaaaagtt tttaaaagcc ataccgcaag *F attctgcatt aaatatatac acacatatat ttatatttcc atttttccgt gcgtatacgt *F gatctgcgtc gaagtcagtt cattctgtcc atgatttaca aatatattta tacataatty *F gccactgaga gaaccgcgaa gtgtaragtg caagtgaagt tcagtattat ttacaaggcg *F tgaaaatcga aaaagtttac accaaatatt ctgctcaaga actcactact caragtagtc *F caaggattcc agtctctcag cgcaacaatt gtcagcgaaa attcctacga atcttagcca *F gctaaaaact ctaattraag tcagacgaaa gcacaactac raagggttaa aaactctgaa *F agcaagaaag aaattaacta aatattaaag gaatcttcga agtgaacacc aaagcaaaga *F tctacagtat tattttgaat aagaaatata taaaacacac tctaagcgat aaatagagac *F aataaagttg aaataaaaaa accaaattaa atttgtgact ctagcctaaa agtaaacaca *F tanaccctag cttaatt *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003567|gb|AE003567|arm:3L 5083015..5237352 *F estimated-cyto:64C9-64C12 gadfly-seqname:AE003567 *F seq_release:3 *F Length = 154338 *F Score = 1513 bits (785), Expect = 0.0 Genome Map *F Identities = 820/845 (97%), Gaps = 1/845 (0%) *F Strand = Plus / Minus *F Query: 14 aacgccttttcctcatttggcgcaccacaaaagcggaaagcgaccacagagccccaagca 73 *F |||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149622 aacgccttttcctcattttgcgcaccacaaaagcggaaagcgaccacagagccccaagca *F 149563 *F Query: 74 cactgagattgccggagacaaccancagccaagtctgaagccaaaatcgaatcgaagtcc 133 *F |||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| *F Sbjct: 149562 cactgagattgccggagacaaccaacagccaagtctgaagccaaaatcgaatcgaagtcc *F 149503 *F Query: 134 taancgaatatcaaatccaagcccaaaaccaaancctactattaacaaatagccnatagc 193 *F ||| |||||||||||| |||||||||||||||| |||||||||||||||||||| ||||| *F Sbjct: 149502 taatcgaatatcaaattcaagcccaaaaccaaatcctactattaacaaatagccaatagc *F 149443 *F Query: 194 gaggagttcacgaaggagtcctgcaaacacatcctttcaacacagtgcaagctcgccatt 253 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149442 gaggagttcacgaaggagtcctgcaaacacatcctttcaacacagtgcaagctcgccatt *F 149383 *F Query: 254 ttgtgttttttcgaaatagaaaaagtttttaaaagccataccgcaagattctgcattaaa 313 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149382 ttgtgttttttcgaaatagaaaaagtttttaaaagccataccgcaagattctgcattaaa *F 149323 *F Query: 314 tatatacacacatatatttatatttccatttttccgtgcgtatacgtgatctgcgtcgaa 373 *F |||||||| |||||||||||||||||||||||||||||||||||||| |||||||||||| *F Sbjct: 149322 tatatacatacatatatttatatttccatttttccgtgcgtatacgtaatctgcgtcgaa *F 149263 *F Query: 374 gtcagttcattctgtccatgatttacaaatatatttatacataattygccactgagagaa 433 *F |||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||| *F Sbjct: 149262 gtcagttcattctgtccatgatttacaaatatatttatacataattagccactgagagaa *F 149203 *F Query: 434 ccgcgaagtgtaragtgcaagtgaagttcagtattatttacaaggcgtgaaaatcgaaaa 493 *F |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 149202 ccgcgaagtgtaaagtgcaagtgaagttcagtattatttacaaggcgtgaaaatcgaaaa *F 149143 *F Query: 494 agtttacaccaaatattctgctcaagaactcactactcaragtagtccaaggattccagt 553 *F ||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||| *F Sbjct: 149142 agtttacaccaaatattctgctcaagaactcactactcaaagtagtccaaggattccagt *F 149083 *F Query: 554 ctctcagcgcaacaattgtcagcgaaaattcctacgaatcttagccagctaaaaactcta 613 *F ||||||||||||||||||||||||||||||||||||||||||||||| |||| ||||||| *F Sbjct: 149082 ctctcagcgcaacaattgtcagcgaaaattcctacgaatcttagccaactaagaactcta *F 149023 *F Query: 614 attraagtcagacgaaagcacaactacraagggttaaaaactctgaaagcaagaaagaaa 673 *F ||| ||||||||||||||||||||||| ||||||||||||||||||||| |||||||||| *F Sbjct: 149022 attaaagtcagacgaaagcacaactacaaagggttaaaaactctgaaagtaagaaagaaa *F 148963 *F Query: 674 ttaactaaatattaaaggaatcttcgaagtgaacaccaaagcaaagatctacagtattat 733 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148962 ttaactaaatattaaaggaatcttcgaagtgaacaccaaagcaaagatctacagtattat *F 148903 *F Query: 734 tttgaataagaaatatataaaacacactctaagcgataaatagagacaataaagttgaaa 793 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 148902 tttgaataagaaatatataaaacacactctaagcgataaatagagacaataaagttgaaa *F 148843 *F Query: 794 t-nnnnnnnccaaattaaatttgtgactctagcctaaaagtaaacacatanaccctagct 852 *F | ||||||||||||||||||||||||||||||||||||||||| ||||||||| *F Sbjct: 148842 taaaaaaaaccaaattaaatttgtgactctagcctaaaagtaaacacataaaccctagct *F 148783 *F Query: 853 taatt 857 *F ||||| *F Sbjct: 148782 taatt 148778 *F \# *F anon-EST:Posey9 = CG9325 *F >anon-EST:Posey9 *F ggcacgaggg taaagcaaat gaacagataa aaaagggtac aaattcccag gcaactctaa *F cgacaaacaa agatttgttt gaaacaaagg caaacttttg tttttagaca catacgtaca *F agcgtggtac atgcataata cttaaatgca atattttttt agaaatgcaa taaattcgat *F gacaacaact acaatttgat attctaggca tgtgtgtgtt ttgtgaaagc gcgttttatg *F tttttgtgtn ctttngtttn gttttnaaat tccgggcagc cacaatttgc acacaaaaca *F aataaataat tattgtatac atgcagcaat tgtattagtt ttttatataa ataattatat *F atttttatgt acgtaaaact gtttatacat acatatacat atatatattt ataaaaacca *F ataaattatg aagaaaacga aaatccaaaa atacaccacg cccgcattaa aacatgcgat *F tantctgagg aaagcagaaa caacacnatc actgagaatg gttggaattt tccaaaaatt *F atctccaaga atcaaatttc agcatttctt ttgtaagtag atgtgcttga tttgtaatcg *F cttttccatt tataatattc aaatcgatga ataacgaata ctgcacatta actatatacg *F actgccatag catgtaaaac tttaaggcac aacttaaacg catttacatt tcaaattaac *F tgacataaaa ttaaaacccc gatgattaat gccagataaa tcgaatgttt ctgcttttcc *F tacccaaacc caccacca *F Database: dmel_all_transcript_r320 *F >CG9325-RF type=mRNA; *F loc=2R:complement(14461055..14462519,14462746..14462813, *F 14471561..14471632,14471695..14471775,14471844..14472138, *F 14472203..14472313,14473340..14473463,14473525..14474236, *F 14474310..14474793,14474880..14474991,14482277..14482325, *F 14487314..14487814); ID=hts-RF; name=hts-RF; *F db_xref='CG9325,FlyBase:FBgn0004873'; len=4074 *F Length = 4074 *F Genome Map *F Score = 717 bits (373), Expect = 0.0 *F Identities = 382/385 (99%), Gaps = 1/385 (0%) *F Strand = Plus / Plus *F Query: 414 aaaaccaataaattatgaagaaaacgaaaatccaaaaatacaccacgcccgcattaaaac 473 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3627 aaaaccaataaattatgaagaaaacgaaaatccaaaaatacaccacgcccgcattaaaac 3686 *F Query: 474 atgcgattantctgaggaaagcagaaacaacacnatcactgagaatggttggaattttcc 533 *F ||||||||| ||||||||||||||||||||||| |||||||||||||||||||||||||| *F Sbjct: 3687 atgcgattactctgaggaaagcagaaacaacacaatcactgagaatggttggaattttcc 3746 *F Query: 534 aaaaattatctccaagaatcaaatttcagcatttcttttgtaagtagatgtgcttgattt 593 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3747 aaaaattatctccaagaatcaaatttcagcatttcttttgtaagtagatgtgcttgattt 3806 *F Query: 594 gtaatcgcttttccatttataatattcaaatcgatgaataacgaatactgcacattaact 653 *F |||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| *F Sbjct: 3807 gtaatcgcttttccatttataatattcaaatcgatgaataacgaatactgca-attaact 3865 *F Query: 654 atatacgactgccatagcatgtaaaactttaaggcacaacttaaacgcatttacatttca 713 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3866 atatacgactgccatagcatgtaaaactttaaggcacaacttaaacgcatttacatttca 3925 *F Query: 714 aattaactgacataaaattaaaaccccgatgattaatgccagataaatcgaatgtttctg 773 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3926 aattaactgacataaaattaaaaccccgatgattaatgccagataaatcgaatgtttctg 3985 *F Query: 774 cttttcctacccaaacccaccacca 798 *F ||||||||||||||||||||||||| *F Sbjct: 3986 cttttcctacccaaacccaccacca 4010 *F Score = 404 bits (210), Expect = e-112 *F Identities = 217/224 (96%) *F Strand = Plus / Plus *F Query: 9 ggtaaagcaaatgaacagataaaaaagggtacaaattcccaggcaactctaacgacaaac 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3222 ggtaaagcaaatgaacagataaaaaagggtacaaattcccaggcaactctaacgacaaac 3281 *F Query: 69 aaagatttgtttgaaacaaaggcaaacttttgtttttagacacatacgtacaagcgtggt 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3282 aaagatttgtttgaaacaaaggcaaacttttgtttttagacacatacgtacaagcgtggt 3341 *F Query: 129 acatgcataatacttaaatgcaatannnnnnnagaaatgcaataaattcgatgacaacaa 188 *F ||||||||||||||||||||||||| |||||||||||||||||||||||||||| *F Sbjct: 3342 acatgcataatacttaaatgcaatatttttttagaaatgcaataaattcgatgacaacaa 3401 *F Query: 189 ctacaatttgatattctaggcatgtgtgtgttttgtgaaagcgc 232 *F |||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 3402 ctacaatttgatattctaggcatgtgtgtgttttgtgaaagcgc 3445 *F Score = 162 bits (84), Expect = 5e-39 *F Identities = 86/87 (98%) *F Strand = Plus / Plus *F Query: 267 aaattccgggcagccacaatttgcacacaaaacaaataaataattattgtatacatgcag 326 *F ||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||| *F Sbjct: 3480 aaattccgggcagccacaatttgcacacaaaacgaataaataattattgtatacatgcag 3539 *F Query: 327 caattgtattagttttttatataaata 353 *F ||||||||||||||||||||||||||| *F Sbjct: 3540 caattgtattagttttttatataaata 3566 *F \# *F anon-EST:Posey93 = end of dlg1 ? *F >anon-EST:Posey93 *F ggcacgaggt tgcggtctaa ttttatacac accagacaaa tcgaattgca aataaataaa *F taattaagaa agtgacaatg gagaagcaga gctggagatg agatcaactc aaactagata *F actatacata tatttgctga ttaatttatg taaaccaata tacgatttat agccaacgca *F cagaaaatga aaaacaaaaa aaagcaacac atacgaaatt tgttcgaaaa aatttagcta *F aattcaagtt gagtgttaac attataattc atgcgaatat gcatatattc aatttttttt *F tttaactctc catctctcct gctcgccatt atacgaacta cttaaaacat ttttttttaa *F ttaaattttt tgtatattgc aaaaacgtcg acgacgccac agcagcagca gctatggata *F ttatttcaaa aatatataca tataactatt atacgaataa agagaaaaca aattcattat *F aattttcaca atgtatgcgc cgagagcatt gtatatttaa taaacaacct aaacaaaagg *F acaaacatgg aaacaaataa aactatgcga ataatttcac tctaacaaaa tgcaaacaag *F caaccaaaaa *F Database: dmel_all_scaffolds_r310 *F >gadfly|SEG:AE003486|gb|AE003486|arm:X 11111476..11439603 *F estimated-cyto:10B6-10D5 gadfly-seqname:AE003486 *F seq_release:3 *F Length = 328128 *F Score = 858 bits (446), Expect = 0.0 Genome Map *F Identities = 545/597 (91%), Gaps = 9/597 (1%) *F Strand = Plus / Plus *F Query: 9 gttgcggtctaattttatacacaccagacaaatcgaattgcaaataaataaataattaag 68 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 38275 gttgcggtctaattttatacacaccagacaaatcgaattgcaaataaataaataattaag 38334 *F Query: 69 aaagtgacaatggagaagcagagctggagatgagatcaactcaaactagataactataca 128 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 38335 aaagtgacaatggagaagcagagctggagatgagatcaactcaaactagataactataca 38394 *F Query: 129 tatatttgctgattaatttatgtaaaccaatatacgatttatagccaacgcacagnnnnn 188 *F ||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 38395 tatatttgctgattaatttatgtaaaccaatatacgatttatagccaacgcacagaaaat 38454 *F Query: 189 nnnnnnnnnnnnnnngcaacacatacgaaatttgttcgaaaaaatttagctaaattcaag 248 *F |||| |||||||||||||||||||||||||||||||||||||||| *F Sbjct: 38455 gaaaaacaaaaaaaagcaatacatacgaaatttgttcgaaaaaatttagctaaattcaag 38514 *F Query: 249 ttgagtgttaacattataattcatgcgaatatgcatatattcaannnnnnnnnnnaactc 308 *F |||||||||||||||||||||||||||||||||||||||||||| ||||| *F Sbjct: 38515 ttgagtgttaacattataattcatgcgaatatgcatatattcaatttttttttttaactc 38574 *F Query: 309 tccatctctcctgctcgccattatacgaactacttaaaacannnnnnnnnaattaaattt 368 *F ||||||||||||||||||||||||||||||||||||||||| |||||||||| *F Sbjct: 38575 tccatctctcctgctcgccattatacgaactacttaaaacatttttttttaattaaattt 38634 *F Query: 369 tttgtatattgcaaaaacgtcgacgacgccacagcagcagcagctatggatattatttca 428 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 38635 tttgtatattgcaaaaacgtcgacgacgccacagcagcagcagctatggatattatttca 38694 *F Query: 429 aaaatatatacatataactattatacgaataaagagaaaacaaattcattataattttca 488 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 38695 aaaatatatacatataactattatacgaataaagagaaaacaaattcattataattttca 38754 *F Query: 489 caatgtatgcgccgagagcattg-tatatttaataaacaacctaaa--caaaaggacaaa 545 *F ||||||||||||||||||||||| |||||||||||||||||||||| ||||||||||| *F Sbjct: 38755 caatgtatgcgccgagagcattgttatatttaataaacaacctaaaacaaaaaggacaaa 38814 *F Query: 546 c-atgg-aaacaaa--taaaactatgcgaataattt-cactct-aacaaaatgcaaa 596 *F | |||| ||||||| ||||||||||||||||||| |||||| ||||||||||||| *F Sbjct: 38815 caatggaaaacaaaataaaaactatgcgaataatttccactctaaacaaaatgcaaa 38871 *F Score = 33.4 bits (17), Expect = 6.8 Genome Map *F Identities = 17/17 (100%) *F Strand = Plus / Plus *F Query: 428 aaaaatatatacatata 444 *F ||||||||||||||||| *F Sbjct: 148756 aaaaatatatacatata 148772 *F \# *F anon-EST:PoseyA1 = qbert *F >anon-EST:PoseyA1 *F ggcacgagag aatctgtatg cttaatctca accttcaggt tttatagatc ctgagatctc *F gactttcata gggacagaca tggccagatc gacttggcta ttcatcctga tcaagaatat *F atattctcta tgtggtcaga aacgcttcct tctgcctgtt acataaggca attttgccga *F ttgctcatat atttacaatc gtacacattc gggtacaagc cgaaccctgc gttgtccata *F cacagtctct gcagaattac tatcatcttt ttcagtttat tttttaaatt ttcaatatca *F tttgaaaata tatgcttctt acccgttact tgtagagtaa aagggtagag aaggaagcgt *F ttccgactac atgtagtatt tatattcttg atcaggatta acagcaaagt cgatcttacc *F ctctcagccg gtcaacggac aagggattaa caatgcccac ttcgcttatc gttctaattc *F taaagaggta ttgaacatgt gacggcagtg gttgtgggga ttttattagt gagtggtgaa *F atagaaagga attggataaa gcgtattgaa acataataac gtgcaagtgc aagtaaaaca *F aaggaaacaa acgaaatcaa gttcaaattg cttcacaggc gaccgtatat gtgtacatca *F aacgctgata aggcaccggg atcgcttgtg cgggacaatt tacaacaatt ataccaacaa *F gttactgtgt gtgatgaccg tatatgtgta catcaatcgc tgataaggca ccgggatcat *F ccatacaccc taact *F Database: dmel_all_transposon_r320 *F >FBti0019900 type=transposable_element; loc=2R:301100..308748; Genome Map *F ID=FBti0019900; name=qbert{}625; map=41F1-41F1; *F db_xref='qbert{}625,FlyBase:FBan0069900'; len=7649 *F Length = 7649 *F Score = 606 bits (315), Expect = e-173 *F Identities = 315/315 (100%) *F Strand = Plus / Minus *F Query: 481 taaagaggtattgaacatgtgacggcagtggttgtggggattttattagtgagtggtgaa 540 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7390 taaagaggtattgaacatgtgacggcagtggttgtggggattttattagtgagtggtgaa 7331 *F Query: 541 atagaaaggaattggataaagcgtattgaaacataataacgtgcaagtgcaagtaaaaca 600 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7330 atagaaaggaattggataaagcgtattgaaacataataacgtgcaagtgcaagtaaaaca 7271 *F Query: 601 aaggaaacaaacgaaatcaagttcaaattgcttcacaggcgaccgtatatgtgtacatca 660 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7270 aaggaaacaaacgaaatcaagttcaaattgcttcacaggcgaccgtatatgtgtacatca 7211 *F Query: 661 aacgctgataaggcaccgggatcgcttgtgcgggacaatttacaacaattataccaacaa 720 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7210 aacgctgataaggcaccgggatcgcttgtgcgggacaatttacaacaattataccaacaa 7151 *F Query: 721 gttactgtgtgtgatgaccgtatatgtgtacatcaatcgctgataaggcaccgggatcat 780 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 7150 gttactgtgtgtgatgaccgtatatgtgtacatcaatcgctgataaggcaccgggatcat 7091 *F Query: 781 ccatacaccctaact 795 *F ||||||||||||||| *F Sbjct: 7090 ccatacaccctaact 7076 *F Score = 79.5 bits (41), Expect = 4e-15 *F Identities = 43/44 (97%) *F Strand = Plus / Minus *F Query: 425 cagccggtcaacggacaagggattaacaatgcccacttcgctta 468 *F ||||||||||||||||||||||||||||||||||||| |||||| *F Sbjct: 7432 cagccggtcaacggacaagggattaacaatgcccactacgctta 7389 *F Score = 71.8 bits (37), Expect = 8e-13 *F Identities = 41/43 (95%) *F Strand = Plus / Minus *F Query: 736 gaccgtatatgtgtacatcaatcgctgataaggcaccgggatc 778 *F ||||| ||||||| ||||||||||||||||||||||||||||| *F Sbjct: 6562 gaccgaatatgtgcacatcaatcgctgataaggcaccgggatc 6520 *F Score = 69.9 bits (36), Expect = 3e-12 *F Identities = 42/45 (93%) *F Strand = Plus / Minus *F Query: 640 cgaccgtatatgtgtacatcaaacgctgataaggcaccgggatcg 684 *F |||||| ||||||| ||||||| |||||||||||||||||||||| *F Sbjct: 6563 cgaccgaatatgtgcacatcaatcgctgataaggcaccgggatcg 6519 *F Score = 60.3 bits (31), Expect = 2e-09 *F Identities = 39/43 (90%) *F Strand = Plus / Minus *F Query: 736 gaccgtatatgtgtacatcaatcgctgataaggcaccgggatc 778 *F ||||| ||||||||||| ||||||||| ||||||||||| ||| *F Sbjct: 6367 gaccgaatatgtgtacaacaatcgctgttaaggcaccggtatc 6325 *F Score = 58.4 bits (30), Expect = 9e-09 *F Identities = 40/45 (88%) *F Strand = Plus / Minus *F Query: 640 cgaccgtatatgtgtacatcaaacgctgataaggcaccgggatcg 684 *F |||||| ||||||||||| ||| ||||| ||||||||||| |||| *F Sbjct: 6368 cgaccgaatatgtgtacaacaatcgctgttaaggcaccggtatcg 6324 *F \# *F anon-EST:CL1d7 = CG10391 *F >anon-EST:CL1d7 *F ttgaaagaaa ctctnnnnaa atatncncct ctacctatta ttgaacncgt ttnccgcaaa *F act *F Database: dmel_all_transcript_r320 *F >CG10391-RA type=mRNA; *F loc=2L:complement(18629140..18629444,18629509..18629778, *F 18629838..18630068,18630121..18630302,18630364..18630480, *F 18630540..18631067); ID=Cyp310a1-RA; name=Cyp310a1-RA; *F db_xref='CG10391,FlyBase:FBgn0032693'; len=1633 *F Length = 1633 *F Genome Map *F Score = 75.7 bits (39), Expect = 3e-14 *F Identities = 51/61 (83%) *F Strand = Plus / Plus *F Query: 1 ttgaaagaaactctnnnnaaatatncncctctacctattattgaacncgtttnccgcaaa 60 *F |||||||||||||| |||||| | |||||||| |||||||||| |||| ||||||| *F Sbjct: 1052 ttgaaagaaactcttcgcaaatatccacctctacccattattgaacgtgtttgccgcaaa 1111 *F Query: 61 a 61 *F | *F Sbjct: 1112 a 1112 *F \# *F anon-EST:CLB3 = CG6736 *F >anon-EST:CLB3 *F tgcttngaga gtaactttat ttncctccac tagaaatttt acaatgaaat atttggaaat *F ncgattacgt ttatagttag tnnttaagtg tagtaggaaa atnaattgca aatngcct *F Database: dmel_all_transcript_r320 *F >CG6736-RA type=mRNA; *F loc=3L:complement(9760920..9761415,9761477..9761807); Genome Map *F ID=CG6736-RA; name=CG6736-RA; *F db_xref='CG6736,FlyBase:FBgn0036048'; len=827 *F Length = 827 *F Score = 194 bits (101), Expect = 9e-50 *F Identities = 110/118 (93%) *F Strand = Plus / Minus *F Query: 1 tgcttngagagtaactttatttncctccactagaaattttacaatgaaatatttggaaat 60 *F ||||| |||||||||||||||| ||||||||||||||||||||||||||||||||||||| *F Sbjct: 812 tgcttggagagtaactttatttgcctccactagaaattttacaatgaaatatttggaaat 753 *F Query: 61 ncgattacgtttatagttagtnnttaagtgtagtaggaaaatnaattgcaaatngcct 118 *F ||||||||||||||||||| ||||||||||||||||||| |||||||||| |||| *F Sbjct: 752 acgattacgtttatagttaggggttaagtgtagtaggaaaatgaattgcaaatagcct 695 *F \# # *U FBrf0178762 *a Rana *b D. *t 2004.7.8 *T personal communication to FlyBase *u *F Date: Thu, 08 Jul 2004 12:30:17 \+0100 *F From: Debashis Rana *F To: 'Gillian Millburn (Genetics)' *F Subject: Re: help with another BLAST ? *F Hi Gillian, *F .. This *F data was generated with dmel_all_transcripts_3.2.0 dataset with an e *F value of 100. *F Cheers, *F Debashis *F FlyBase curator comment: BLAST output file archived as Rana.2004.7.8-1.txt *F File deposited: Rana.2004.7.8-1.txt ; File date: 2004.7.16 ; File size: *F 3560586 ; File format: txt # *U FBrf0178763 *a Steward *b R. *t 2004.6.30 *T personal communication to FlyBase *u *F Date: Wed, 30 Jun 2004 12:19:22 \-0400 *F From: Ruth Steward *F To: 'Michael Ashburner (Genetics)' *F Subject: Re: flex-bb *F Content-Transfer-Encoding: 7bit *F Michael, *F Based on new experiments it appears most probable that flex is an *F allele of bb. *F As I mentioned before we also sequenced the insertion site of *F P{FRT(whs)}9-2 and found that it is inserted at 18C1 and not 18E as *F listed in flybase. We could send you the sequence if you would like to *F put it into flybase. *F Thanks for your help. Ruth *F Ruth Steward *F Molecular Biology and Biochemistry Professor *F Waksman Institute *F Rutgers University *F 190 Frelinghuysen Rd *F Piscataway, NJ 08854-8020 *F Tel: 732.445.3917 *F FAX: 732.445.5735 *F steward@waksman.rutgers.edu # *U FBrf0178764 *a Ashburner *b M. *c S. *d Marygold *t 2004.7.2 *T personal communication to FlyBase *u Ribosomal proteins. *F Date: Fri, 02 Jul 2004 18:10:59 \+0100 *F Subject: Re: Ribosomal proteins *F From: Steven Marygold *F To: 'Michael Ashburner (Genetics)' *F Dear Michael, *F Thanks a lot for getting back to me on this- I appreciate your time. I *F agree that most RPs are correctly identified and named in FlyBase, though as *F you say, there are a few that could do with tweaking. *F I've attached my comments on the list you sent me. To date, I've only *F considered the cyto RPs. I made my compilation of the D.m. RPs by combining *F my own gene ontology searches with the RPG data gleaned from BLAST hits *F against human RPs. In comparing my compilation to your list, there are only *F 2-3 that don't appear in both lists. In my comments, I've assumed that the *F RPG classification and nomenclature conventions are the correct ones. *F Therefore there is no recognised L1, L2, L16, L20, L25, L33, S1 or S22 RP *F classification. I think the convention with the fly RPs is that they're *F named after their similarity to vertebrate/mammalian RPs, and I've followed *F this in my comments. I've also added a few ideas for fly gene name changes- *F though I'm not sure of the FlyBase policy on this. *F ... *F Steven. *F \-- *F Steven Marygold Ph.D. *F Growth Regulation Laboratory (Rm 503), *F Cancer Research UK London Research Institute, *F 44 Lincoln¹s Inn Fields, *F LONDON, WC2A 3PX *F U.K. *F Tel: \+44 (0)20 7269 3351 *F Fax: \+44 (0)20 7269 3581 *F L1 RpL10Aa CG3843 *F L1 RpL10Ab CG7283 *F These genes are shown as RpL10A orthologs in RPG- there is no 'RPL1' *F classification. The confusion stems from these proteins having a 'Ribosomal L1 *F motif', which I think refers to the prokaryotic homologs; this corresponds to *F RPL10A in mammals. *F L3 RpL3 CG4863 *F L4 RpL4 CG5502 *F L5 RpL11 CG7726 *F This gene is the ortholog of mammalian RPL11. Again, I think there's confusion *F from the protein having a 'Ribosomal L5' motif, which corresponds to mammalian *F RPL11. CG17489 is the real RPL5 ortholog (below). *F L5 yip6 CG17489 *F I recommend this gene be renamed 'RpL5'. *F L6 RpL6 CG11522 *F L7 RpL7 CG4897 *F L7-like CG5317 CG5317 *F (CG4897 protein is 55% identical to human RPL7 and CG5317 is only 28% identical, *F so it's probably right to refer to the latter as 'L7-like' rather than L7b.) *F L7A RpL7A CG3314 *F L8 RpL8 CG1263 *F L9 RpL9 CG6141 *F L10 Qm CG17521 *F I recommend this gene be renamed 'RpL10'. *F L11 RpL12 CG3195 *F The real RPL11 ortholog is CG7726 (as in FlyBase). CG3195 is the RPL12 *F ortholog; again, I think the confusion from CG3195 protein having a prokaryotic *F 'Ribosomal L11 motif' when it actually corresponds to mammalian L12. *F L13 RpL13A CG1475 *F This gene is the RpL13A ortholog. *F L13E RpL13 CG4651 *F This gene is just the 'RPL13' ortholog. *F L14 RpL14 CG6253 *F L15 RpL15 CG17420 *F L17 RpL23 CG3661 *F This gene is the RPL23 ortholog. It appears to have been mis-named in the past *F as L17 or L17A for some reason. *F L18 RPL18 CG8615 *F L18A RPL18A CG6510 *F L19e RpL19 CG2746 *F This gene is just the 'RPL19' ortholog. *F L21 oho23B CG2986 *F This is wrong. oho23B is the RPS21 ortholog. *F L21E RpL21 CG12775 *F This gene is just the 'RPL21' ortholog. *F L22 RpL22 CG7434 *F L22-like CG9871 CG9871 *F (CG7434 protein is 58% identical to human RPL22 and CG9871 is 39% identical, so *F it's probably right to refer to the latter as 'L22-like' rather than L22b.) *F L23/L17-like CG3203 CG3203 *F This is the real L17 ortholog (see above). *F L23A RpL23A CG7977 *F L24 RpL24 CG9282 *F L24-like CG6764 CG6764 *F (CG9282 protein is 65% identical to human RPL24 and CG6764 is only 22% *F identical, so it's probably right to refer to the latter as 'L24-like' rather *F than L24b.) *F L26 RpL26 CG6846 *F L27 RpL27 CG4759 *F L28 RpL28 CG12740 *F L29 RpL29 CG10071 *F L27A RpL27A CG15442 *F L30 RpL30 CG10652 *F The L31 ortholog (CG1821) is missing, though it's in FlyBase. *F . *F L32 RpL32 CG7939 *F L33 CG15458 CG15458 *F I'm not at all sure about this one. There's no L33 classification in the RPG. *F There's a L33 reference in the CG15458 FlyBase record, but a SMART search shows *F the protein sequence has a transmembrane domain and a Pfam search shows it has *F no Pfam domains. *F L34 RpL34 CG6090 *F L34-like CG9354 CG9354 *F I think these two should probably be renamed L34a and L34b as both protein *F products are 52% identical to human RPL34. *F L35 RpL35 CG4111 *F L35A RpL35A CG2099 *F L36 RpL36 CG7622 *F L36A RpL36A CG7424 *F L37 RpL37 CG9091 *F L37-like CG9873 CG9873 *F There's also a case for these two being renamed L37a and L37b as CG9091 is 74% *F identical to human RPL27 and CG9873 is 65% identical. *F L37A RpL37A CG5827 *F L38 RpL38 CG18001 *F This has an updated CG no. of CG40278 *F L39 RpL39 CG3997 *F L40 RpL40 CG2960 *F L41 RpL41 CR30425 *F Clearly this is the RPL41 homolog and not a non-coding RNA (CR). *F P0 RpLP0 CG7490 *F P2 RpLP1 CG4087 *F P1 RpLP2 CG4918 *F The RPG now classifies these as RPLP0, RPLP1, and RPLP2 (like the fly gene names *F in fact.) *F SA sta CG14792 *F I recommend this be renamed RpSA. *F S2 sop CG5920 *F I recommend this be renamed RpS2. *F S3 RpS3 CG6779 *F S3A RpS3A CG2168 *F S4 RpS4 CG11276 *F S4-like CG4866 CG4866 *F I'm not sure what this is but it's not an ortholog of RPS4- it only shares 3% *F identity with human S4 compared to the 75% identity of CG11276 and human RPS4. *F It has a prokaryotic ribosomal S4 domain that actually corresponds to eukaryotic *F S9, but it's only 16% identical to human RPS9 (compared to 84% identity between *F human S9 and CG3395- see below). *F S5 RpS5a CG8922 *F S5 RpS5b CG7014 *F S6 RpS6 CG10944 *F S6-like CG11386 CG11386 *F This is the only one on your list that I didn't find at all in my original Dm RP *F searches. It looks like S6 (it's 40% identical to human S6 whereas CG10944 is *F 75% identical) and it has a Pfam Ribosomal S6e domain. *F S7 RpS7 CG1883 *F S8 RpS8 CG7808 *F S9 RpS9 CG3395 *F S10 RpS10a CG12275 *F S10 RpS10b CG14206 *F S11 RpS11 CG8857 *F S12 RpS12 CG11271 *F S13 RpS13 CG13389 *F S14 RpS14a CG1524 *F S14 RpS14b CG1527 *F S15 RpS15 CG8332 *F S15A RpS15Aa CG2033 *F S15A RpS15Ab CG12324 *F S16 RpS16 CG4046 *F S17 RpS17 CG3922 *F S18 RpS18 CG8900 *F S19 RpS19a CG4464 *F S19 RpS19b CG5338 *F S20 RpS20 CG15693 *F The RPS21 ortholog is CG2986/oho23B from your RP-L list above. I recommend this *F gene be renamed RpS21. *F S23 RpS23 CG8415 *F S24 RpS24 CG3751 *F S25 RpS25 CG6684 *F S26 RpS26 CG10305 *F S27 RpS27 CG10423 *F S27A RpS27A CG5271 *F S28 RpS28a CG15527 *F S28 RpS28b CG2998 *F S29 RpS29 CG8495 *F S30 RpS30 CG15697 *F Other structural components of cytosolic ribosome *F CG32276 CG32276 ribosome associated membrane protein-like *F CG1789 CG1789 ? *F hoip CG3949 Trisn small nuclear ribonucleoprotein *F Other genes in FB said to encode ribosomal proteins, but with no sequence data *F &, therefore, not mapped to genome. *F S11-like anon-MMS23 *F 7/8 RP7-8 *F 34 Rp21 *F 34 Rp34 *F ? RpL5 *F RpL5 and yip6 reports should be merged. # *U FBrf0178765 *a Ashburner *b M. *t 2004.7.2 *T personal communication to FlyBase *u *F Date: Fri, 2 Jul 2004 17:47:49 \+0100 (BST) *F From: 'Michael Ashburner (Genetics)' *F To: gm119@gen.cam.ac.uk *F \*a mRpL-CI-B8 *F please rename as *F \*a mRpL43 *F Here is the evidence: *F >gi|17505717|ref|NP_492686.1| LocusLink info mitochondrial ribosomal protein *F L43 (1K769) Caenorhabditis elegans *F gi|7496448|pir||T19441 hypothetical protein C25A1.13 \- Caenorhabditis elegans *F gi|3874440|emb|CAB02765.1| Hypothetical protein C25A1.13 Caenorhabditis elegans *F Length = 189 *F Score = 115 bits (287), Expect = 6e-25 *F Identities = 61/160 (38%), Positives = 96/160 (60%), Gaps = 1/160 (0%) *F Query: 10 SGFPRAPLQNGLGRYVCQLQRITLKFCKNNGSSRGMRDFIENHLLDFAKENPGIVVYVKP 69 *F S F \+ P NG+ RY QL RIT++FCK \+ SS G+R+FIEN L++ \++NP \+V+Y \+P *F Sbjct: 27 SDFLKIPAYNGISRYTNQLHRITVRFCKQSESSVGVRNFIENQLVEIGRKNPSVVIYAQP 86 *F Query: 70 RRHRTPVLVGEYLNGEREWMSCRNSTQEEISKWIDLLKTQNGSSSSLRLRKMWHTEVPSI 129 *F R+ P \+ EY NG \++ \+N TQ+E+SK \+ LL \+++G \++L VPSI *F Sbjct: 87 VRNSNPSIRAEYGNGRTLQINAKNMTQDEVSKDVHLLFSRSG-EPVVKLESRQSALVPSI 145 *F Query: 130 QGPWTPFLLRSPDAQGQAYPSAEASKPLDTPQTATEKLIE 169 *F QG WTP \+ \+ P+ \+ SK T \+ATE \+++ *F Sbjct: 146 QGQWTPITWLPTEMNTEQLPAKQFSKHKSTATSATEYILQ 185 *F >gi|30962909|gb|AAH52639.1| LocusLink info MRPL43 protein Homo sapiens *F Length = 164 *F Score = 111 bits (278), Expect = 6e-24 *F Identities = 59/127 (46%), Positives = 79/127 (62%), Gaps = 1/127 (0%) *F Query: 10 SGFPRAPLQNGLGRYVCQLQRITLKFCKNNGSSRGMRDFIENHLLDFAKENPGIVVYVKP 69 *F S F \+ L NGLGRYV QLQR++ \++ SSRG R+F+E \++DFA+ NPG+V+YV *F Sbjct: 8 SRFLASVLHNGLGRYVQQLQRLSFSVSRDGASSRGAREFVEREVIDFARRNPGVVIYVNS 67 *F Query: 70 RRHRTPVLVGEYLNGEREWMSCRNSTQEEISKWIDLLKTQNGSSSSLRLRKMWHTEVPSI 129 *F R P \+V EYLNG S \+ EEIS \+ L Q+G \+R+RK \+HT+ PSI *F Sbjct: 68 RPCCVPRVVAEYLNGAVREESIHCKSVEEISTLVQKLADQSG-LDVIRIRKPFHTDNPSI 126 *F Query: 130 QGPWTPF 136 *F QG W PF *F Sbjct: 127 QGQWHPF 133 # *U FBrf0178766 *a Ashburner *b M. *t 2004.7.2 *T personal communication to FlyBase *u *F Date: Fri, 2 Jul 2004 14:57:15 \+0100 (BST) *F From: 'Michael Ashburner (Genetics)' *F Subject: more ribosomal prot cleans *F To: gm119@gen.cam.ac.uk *F I have just done some more work on the genes said to have proteins *F that are 'ribosomal protein \*-like' *F ... *F This is a mitochondrial ribosomal protein *F rename *F \*a CG4866 *F > *F \*a mRpS4 *F ============================================================================== *F >CG4866-PA feature: protein *F MVRKLKFHEQKLLKKVDFITWKVDNGGKENKILRRFHIQKREDYTKYNKL *F SREIRELAERIAKLDASEPFKTEATTMLLNKLHAMGVSNDQLTLETAAKI *F SASHFCRRRLPVIMVKLRMSEHLKAATDLIEHGHVRVGPEMIKDPAFLVS *F RNLEDFVTWVDGSKIKEHVLRYNDMRDDFQM *F >RpS4-PA feature: protein *F MARGPKKHLKRLAAPKAWMLDKLGGVFAPRPSTGPHKLRESLPLLIFLRN *F RLKYALNGAEVTKIVMQRLVKVDGKVRTDPTYPAGYMDVITLEKTGEFFR *F LVYDVKGRFVIHRISAEEAKYKLCKVKKTQLGAKGVPFLVTHDGRTIRYP *F DPLIHANDSVQVDIASGKITDYIKFDSGNLCMITGGRNLGRVGTVVNRER *F HPGSFDIVHIKDSQGHVFATRLTNVFIIGKGNKPYISLPKGKGVKLSIAE *F ERDKRLAAKTH *F >RpS4-PB feature: protein *F MARGPKKHLKRLAAPKAWMLDKLGGVFAPRPSTGPHKLRESLPLLIFLRN *F RLKYALNGAEVTKIVMQRLVKVDGKVRTDPTYPAGYMDVITLEKTGEFFR *F LVYDVKGRFVIHRISAEEAKYKLCKVKKTQLGAKGVPFLVTHDGRTIRYP *F DPLIHANDSVQVDIASGKITDYIKFDSGNLCMITGGRNLGRVGTVVNRER *F HPGSFDIVHIKDSQGHVFATRLTNVFIIGKGNKPYISLPKGKGVKLSIAE *F ERDKRLAAKTH *F CG4866-PA MVRKLKFHEQKLLKKVDFITWKVDN-GG--KENK-----ILRR---FHIQKREDYTKYNK *F RpS4-PA MARGPKKHLKRLAAPK---AWMLDKLGGVFAPRPSTGPHKLRESLPLLIFLR-NRLKYAL *F RpS4-PB MARGPKKHLKRLAAPK---AWMLDKLGGVFAPRPSTGPHKLRESLPLLIFLR-NRLKYAL *F \*.\* \* \* ::\* :\* :\*: \*\* . \*\*. : \* *F \* : \*\* *F CG4866-PA LSREIRELA-ERIAKLD---ASEP-FKT--------EAT---TMLLN--K----LHAMGV *F RpS4-PA NGAEVTKIVMQRLVKVDGKVRTDPTYPAGYMDVITLEKTGEFFRLVYDVKGRFVIHRISA *F RpS4-PB NGAEVTKIVMQRLVKVDGKVRTDPTYPAGYMDVITLEKTGEFFRLVYDVKGRFVIHRISA *F . \*: ::. :\*:.\*:\* ::\* : : \* \* \*: \* *F :\* :.. *F CG4866-PA SNDQLTLETAAK--ISAS--HFCR----R--RLP--VIMV--KLRMSEHLKAATDLIEH- *F RpS4-PA EEAKYKLCKVKKTQLGAKGVPFLVTHDGRTIRYPDPLIHANDSVQVDIASGKITDYIKFD *F RpS4-PB EEAKYKLCKVKKTQLGAKGVPFLVTHDGRTIRYPDPLIHANDSVQVDIASGKITDYIKFD *F .: : .\* .. \* :.\*. \* \* \* \* :\* . .:::. *F \*\* \*:. *F CG4866-PA \-G---HV-------RVG--------P---E--MIKD---P--------AFLVSRNLEDFV *F RpS4-PA SGNLCMITGGRNLGRVGTVVNRERHPGSFDIVHIKDSQGHVFATRLTNVFIIGKGNKPYI *F RpS4-PB SGNLCMITGGRNLGRVGTVVNRERHPGSFDIVHIKDSQGHVFATRLTNVFIIGKGNKPYI *F \* : \*\*\* \* : \*\*\* *F .\*::.:. : :: *F CG4866-PA TWVDGSKIKEHVLRYNDMRDDFQM- *F RpS4-PA SLPKGKGVKLSIAEERDKRLAAKTH *F RpS4-PB SLPKGKGVKLSIAEERDKRLAAKTH *F : .\*. :\* : . .\* \* : *F >gi|8922794|ref|NP_060755.1| chromosome 15 open reading frame 12; mitochondrial *F ribosomal *F protein S4 Homo sapiens *F gi|7023341|dbj|BAA91929.1| unnamed protein product Homo sapiens *F gi|12053321|emb|CAB66847.1| hypothetical protein Homo sapiens *F gi|13623715|gb|AAH06487.1| Chromosome 15 open reading frame 12 Homo sapiens *F gi|15079901|gb|AAH11745.1| C15orf12 protein Homo sapiens *F gi|15808676|gb|AAL06639.1| BRMS2 Homo sapiens *F Length = 184 *F Score = 209 bits (531), Expect = 3e-53 *F Identities = 104/182 (57%), Positives = 143/182 (78%), Gaps = 1/182 (0%) *F Query: 1 MVRKLKFHEQKLLKKVDFITWKV-DNGGKENKILRRFHIQKREDYTKYNKLSREIRELAE 59 *F MVRKLKFHEQKLLK+VDF+ W+V D+ E \++LRR+ \+Q+REDYT+YN+LSR \+RELA *F Sbjct: 1 MVRKLKFHEQKLLKQVDFLNWEVTDHNLHELRVLRRYRLQRREDYTRYNQLSRAVRELAR 60 *F Query: 60 RIAKLDASEPFKTEATTMLLNKLHAMGVSNDQLTLETAAKISASHFCRRRLPVIMVKLRM 119 *F R+ L \+ F+ A+ LL+KL+A+G+ \+ \+LE \++AS FCRRRLP \+++KLRM *F Sbjct: 61 RLRDLPERDQFRVRASAALLDKLYALGLVPTRGSLELCDFVTASSFCRRRLPTVLLKLRM 120 *F Query: 120 SEHLKAATDLIEHGHVRVGPEMIKDPAFLVSRNLEDFVTWVDGSKIKEHVLRYNDMRDDF 179 *F \++HL+AA \+E GHVRVGP+++ DPAFLV+R++EDFVTWVD SKIK HVL YN+ RDDF *F Sbjct: 121 AQHLQAAVAFVEQGHVRVGPDVVTDPAFLVTRSMEDFVTWVDSSKIKRHVLEYNEERDDF 180 *F Query: 180 QM 181 *F \+ *F Sbjct: 181 DL 182 *F ============================================================================== # *U FBrf0178767 *a Ponting *b C. *t 2004.6.17 *T personal communication to FlyBase *u VKOR in Drosophila. *F From: 'Chris Ponting' *F To: ''Michael Ashburner \(Genetics\)'' *F Subject: RE: VKOR in Drosophila *F Date: Thu, 17 Jun 2004 17:03:27 \+0100 *F Hi Michael, *F Thanks for your email. Here are the region, the DNA and the protein. *F Many thanks! *F Best wishes, *F Chris *F 2R \++ 11842090 11842655 *F ATGGAACAGG CGTACAGTAC AGCTTCCCGA CTGCGTGGGA TTTGCGTTTG TGGATTGGCC *F 11842149 *F ATTTCGGTGT ATTCACTGTA TGTGAAAATG AAACTAAAGG AGGATGAGAA CTATAGGCCA *F 11842209 *F ATGTGCGACG TTAACGATAA TATAAGCTGC TCGCTGGTTT TTAAATCGGG Gtaggtaaga *F 11842269 *F ttataatata tacaatataa atttaatgaa ataattttcc atcagcTATG GTGATGGCTT *F 11842329 *F TGGTCTGGGT AACATAACCC AAGTAAATGC TCCCAACGGA GCCATCGGCT GTGCATTTTA *F 11842389 *F TATCCTGTAC TTCTTGagct gtacgtaaat ttcttagtac aaactaaact aaatttaata *F 11842449 *F agcatttgtt tTCAGCTTTC TTTAATCATC GTTGGCTGTG CCTGGTCCAA TTGATAGTAT *F 11842509 *F GCACTTTGAC ATTACTTCTC TGCGTTTATC TGGGTTTCCT GCTGATTCTC GTGTTTTACG *F 11842569 *F ATTTCTGTTT GGTATGCGTG ACCATCTATT TCATACACAC ATGGCTCTTT CAGGAAGTCC *F 11842629 *F TAAGGCGATA TAGACGCCTT TATATG *F MEQAYSTASR LRGICVCGLA ISVYSLYVKM KLKEDENYRP MCDVNDNISC SLVFKSGYGD 60 *F GFGLGNITQV NAPNGAIGCA FYILYFLSAF FNHRWLCLVQ LIVCTLTLLL CVYLGFLLIL 120 *F VFYDFCLVCV TIYFIHTWLF QEVLRRYRRL YM *F \-----Original Message----- *F From: Michael Ashburner (Genetics) mailto:ma11@gen.cam.ac.uk *F Sent: 17 June 2004 16:56 *F To: chris.ponting@anat.ox.ac.uk *F Cc: ma11@gen.cam.ac.uk *F Subject: VKOR in Drosophila *F Chris *F Just reading yr TIBS paper on VKOR. I see where it is on the genome but *F it *F clearly does not match our annotation. If you could send me the data I *F will *F have it curated citing yr paper and a personal communication (for the *F data) *F from you (yr pc will be given an identifier and archived for all to *F see). *F Thanks *F Michael # *U FBrf0178768 *a Wagstaff *b B. *t 2004.7.12 *T personal communication to FlyBase *u FlyBase error report for unannotated, 53C region on Mon Jul 12 18:17:49 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Mon, 12 Jul 2004 18:17:49 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: bwagstaff@mail.utexas.edu *F Subject: FlyBase error report for unannotated, 53C region on Mon Jul 12 *F 18:17:49 2004 *F Error report from Bradley Wagstaff (bwagstaff@mail.utexas.edu) *F Gene or accession: unannotated, 53C region *F Missed gene *F Comments: I have detected an unannotated tandem duplicate to Acp53Ea. There are *F 519bp between the termination codon of the unannotated gene and the initiation *F codon of Acp53Ea. This new gene is discussed in Holloway, A. K. and D. J. Begun *F 2004, Mol. Biol. Evol. 21(9):pages yet to be determined. The name chosen, based *F on putative function, location, and structure is 'Acp53C14c'. The open reading *F frame includes a first exon ATGAAGTCCAAACAAGTCTTTTACATCGCCTTCAGCTTGCTT, a 57bp *F intron, then a final exon *F CTGCTGGGATCCTTGCTGCCAAACGAAGTGGAGTCCTTAAGAGTAGATCTTAATAAGCTGGCGGAGTGCACAGAATCGGG *F ATTGAAAGTAGCCACAACGTTGCTCGTGAGGGCGATACCATGTGTAAAAAAATTGGCAAAATGTGCTGACTTTCGAGCCA *F TTAAGACTAAGGACCTTGATATTACCGCACTTGCCCTCTTGGGCTATCAATATCTGCAAACGGTCGTGAACAACCAAAGA *F TGTCTATTGATCTCATTGAAAGAAGGCTACGACGCTGTGTCGCCACACCTTGACAAACTTATTAGTGGCAAGTGCCTACC *F AGGGCTCAGCTAA *F I have RACE data to support the gene and annotation I have just described. *F Please let me know if there is anything else I should do. *F Thanks, *F Bradley Wagstaff # *U FBrf0178769 *a Dallman *b J. *t 2004.6.23 *T personal communication to FlyBase *u FlyBase error report for CG32535 on Wed Jun 23 11:34:02 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Wed, 23 Jun 2004 11:34:02 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: jdallman@notes.cc.sunysb.edu *F Subject: FlyBase error report for CG32535 on Wed Jun 23 11:34:02 2004 *F Error report from Julia Dallman (jdallman@notes.cc.sunysb.edu) *F Gene or accession: CG32535 *F Gene annotation error *F Genes CG32535 and CG3878 should be merged. *F Comments: In sequencing ESTs that corresponded to CG3878, there were two *F splicevariants that also contained CG32535 at their 3' end therefore, I do not *F think that CG32535 is a unique gene. There were three splicevariants in *F total, CG3878 being the shortest. # *U FBrf0178770 *a Holloway *b A. *t 2004.4.29 *T personal communication to FlyBase *u FlyBase error report for CG17011 on Thu Apr 29 13:38:08 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Thu, 29 Apr 2004 13:38:08 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: aholloway@mail.utexas.edu *F Subject: FlyBase error report for CG17011 on Thu Apr 29 13:38:08 2004 *F Error report from Alisha Holloway (aholloway@mail.utexas.edu) *F Gene or accession: CG17011 *F Gene annotation error *F Gene CG17011 has incorrect exon/intron structure. *F Comments: We have RT-PCR data showing that the initiation codon is 787 bases *F 3' of the currently annotated form of this gene. There is no intron in *F CG17011. This gene is related to Acp29AB and Lectin29Ca. Using the new *F annotation for CG17011, all three of these genes exhibit similar intron/exon *F structure, gene length, predicted function, and are predicted to be secreted *F proteins. # *U FBrf0178771 *a Scholz *b H. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:47:37 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10449 *F To: henrike.scholz@biozentrum.uni-wuerzburg.de *F Cc: rd120@gen.cam.ac.uk *F Dear Henrike, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The hangover gene defines a stress pathway required for ethanol tolerance in *F Drosophila *F You mention a gene symbol that is new to FlyBase, hangover. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if hangover has picked up a short symbol since you submitted the *F abstract then this would be a good time to tell me so I can get it into *F the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 11:09:57 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Henrike Scholz *F Subject: Re: Helping FlyBase: ADRC-10449 *F Dear Rachel, *F hangover (short: hang) corresponds to CG32575 (CG4411). Since it is in the *F process of getting published, I was a little bit imprecise. *F .... *F With best wishes *F Henrike *F \---------------------------------------------------------------------- *F Dr. Henrike Scholz *F Biozentrum, Lehrstuhl für Genetik und Neurobiologie *F University of Würzburg *F Am Hubland *F D- 97074 Würzburg *F Germany *F Phone (office): 49 0931 888 4479 *F Phone (lab) : 49 0931 888 4484 *F Fax: 49 0931 888 4452 *F email: henrike.scholz@biozentrum.uni-wuerzburg.de # *U FBrf0178772 *a Casas *b S. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:16:32 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10051 *F To: scasas@cib.csic.es *F Cc: rd120@gen.cam.ac.uk *F Dear Sergio, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Cloning and characteriztion of DmMNF: A new transcription factor of the fork *F head family in Drosophila melanogaster. *F You mention a gene symbol that is new to FlyBase, Mnf (we drop the DM, *F see copy mailing below explaining why). Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. I'm wondering whether it might be CG11799? We have *F EP3428 as an allele of CG11799.... *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: kaufman@bio.indiana.edu (T. Kaufman) *F Newsgroups: bionet.drosophila *F Subject: Nomenclature *F Date: 17 Mar 1999 08:24:52 \-0800 *F ============= *F Dear Colleagues, *F We are writing with a suggestion about the nomenclature of Drosophila *F genes. *F It is now routine that a D. melanogaster gene is identified on the *F basis of sequence homology with (or more rarely, functional *F complementation for) a gene from another organism such as a yeast or a *F vertebrate. Authors often prefix these Drosophila versions with 'D' or 'd' *F for Drosophila, or 'Dm' for D. melanogaster. However in the absence of a *F unifying pan-biological system of genetic nomenclature, to haphazardly *F use 'D' or 'd' or 'Dm' is actually counterproductive. Think of Danio, *F Dictyostelium, Daphnia magna .... there is room for confusion. *F FlyBase uses a defined system of prefixes for denoting species other *F than D. melanogaster. Examples are 'Dvir\' for D. virilis, 'Dsim\' for D. *F simulans, etc. Examples of gene symbols are Dvir\Adh1 and Dsim\per *F (see flybase/Documents/nomenclature/species-abbreviations.txt for full *F listing of species abbreviations). We have not explicitly listed *F melanogaster genes with the prefix 'Dmel\' since we consider that to be *F implicit. However we would like to suggest that when meaning to *F distinguish between a vertebrate gene and its Drosophila version that *F would otherwise have the same symbol, authors prefix the symbol with *F 'Dmel\'. 'D', 'd', or 'Dm' ought NOT to be used as a prefix to a *F D. melanogaster gene symbol \- this is a long-standing convention, having *F been made explicit by Lindsley and Zimm in the 1992 edition of the Red *F Book. *F FlyBase *F ============ *F From: 'Sergio Casas' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10051 *F Date: Mon, 23 Feb 2004 12:09:51 \+0100 *F Dear Rachel, *F i have just read your e-mail, Mnf corresponds to CG11799, and Ep3428 is an *F allele of CG11799, the gene that we are studing (CG11799) is the most *F homolog to vertebrate MNF and that is the reason because we named it *F 'DmMNF'. *F So, to avoid duplications, note that both CG11799 (Ep3428) and Mnf refers to *F the same gene. *F We didn´t know anything about the new nomenclature, in the future, we will *F avoid the 'Dm' *F Best wishes. *F Sergio # *U FBrf0178773 *a Faria *b M. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:32:50 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10134 *F To: mfaria@igc.gulbenkian.pt *F Cc: rd120@gen.cam.ac.uk *F Dear Mariana, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Cloning and characterization of Drosophila Mps1 protein kinase *F You mention a gene symbol that is new to FlyBase, Mps1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Mariana Faria' *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10134 *F Date: Mon, 23 Feb 2004 11:18:45 \-0000 *F Dear Rachel *F Mps1 corresponds to CG7643. It is described as the homolog of Saccharomyces *F cerevisiae Mps1 *F Best wishes *F Mariana # *U FBrf0178774 *a Sezgin *b E. *t 2004.2.23 *T personal communication to FlyBase *u *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 23 Feb 2004 09:32:57 \+0000 *F Date: Mon, 23 Feb 2004 09:31:40 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10103 *F To: e_sezgin@life.bio.sunysb.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Efe, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Population genetics of trehalose pathway genes in Drosophila melanogaster and *F Drosophila simulans. *F You mention a gene symbol that is new to FlyBase, UDPGPP. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 23 Feb 2004 14:13:47 \+0000 *F From: 'efe sezgin' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10103 *F Date: Mon, 23 Feb 2004 09:13:25 \-0500 *F Dear Rachel, *F Thank you for your warning email. This issue of different names for the *F genes is a real problem and I hope it will disappear by time trough *F integration of data in the flybase. I used the symbol UDPGPP because this *F was one of the names used couple of years ago. Now I checked Flybase again. *F I think using the symbol UGP (which stands for UTP-glucose-1-phosphate *F uridyltransferase) will be more appropriate. *F ... *F Thank you very much for your time and concerns, *F EFE SEZGIN *F Dept. of Ecology and Evolution *F SUNY at Stony Brook *F Stony Brook, NY 11794-5245 *F lab: 631/632-8586 *F fax: 631/632-7626 *F e_sezgin@life.bio.sunysb.edu *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 23 Feb 2004 15:11:46 \+0000 *F Date: Mon, 23 Feb 2004 15:10:56 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10103 *F To: rd120@gen.cam.ac.uk, e_sezgin@life.bio.sunysb.edu *F Dear Efe, *F UGP should be fine for a gene symbol though I's still like to know if *F you know which CG UGP corresponds to, provided that this is information *F that you are happy to have made public. *F Many thanks for your prompt reply. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Tue, 24 Feb 2004 00:34:44 \+0000 *F From: 'efe sezgin' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10103 *F Date: Mon, 23 Feb 2004 19:34:21 \-0500 *F Dear Rachel, *F I am sorry I forgot to mention the CG of the gene. The coding gene code for *F UGP is CG4347. *F thanks, *F EFE SEZGIN *F Dept. of Ecology and Evolution *F SUNY at Stony Brook *F Stony Brook, NY 11794-5245 *F lab: 631/632-8586 *F fax: 631/632-7626 *F e_sezgin@life.bio.sunysb.edu # *U FBrf0178775 *a Sato *b A. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:39:27 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10230 *F To: as2047@columbia.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Atsushi, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization and functional analysis of the Drosophila Corin Protein *F You mention a gene symbol that is new to FlyBase, Corin. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: Atsushi Sato *F Date: Mon, 23 Feb 2004 09:14:59 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10230 *F Dear Rachel, *F I'm sorry for confusing you. *F Corin is CG2105. *F Thank you always keeping new. *F Sincerely, *F Atsushi *F \################################################### *F Atsushi Sato Ph.D. *F Department of Genetics and Development *F College of Physicians and Surgeons *F Columbia University *F 701 West 168th Street, HHSC 1120 *F New York, NY 10032, USA *F Phone: \+1-212-305-3192 *F FAX: \+1-212-342-6902 *F E-mail: atsushi.sato@columbia.edu *F \################################################### # *U FBrf0178776 *a Certel *b S. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:18:31 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10597 *F To: scertel@hms.harvard.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Sarah, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Regulation of axon outgrowth and fasciculation by the matricellular protein, *F dCCN. *F You mention a gene symbol that is new to FlyBase, Ccn. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Subject: RE: Helping FlyBase: ADRC-10597 *F Date: Mon, 23 Feb 2004 09:43:01 \-0500 *F From: 'Certel, Sarah ' *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F What I've temporarily termed dCCN corresponds to CG32183 according to the *F Genome Project. *F Thanks, *F Sarah # *U FBrf0178777 *a Reed *b B. *t 2004.2.23 *T personal communication to FlyBase *u *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Mon, 23 Feb 2004 09:27:09 \+0000 *F Date: Mon, 23 Feb 2004 09:26:12 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10784 *F To: reed@sickkids.on.ca *F Cc: rd120@gen.cam.ac.uk *F MIME-Version: 1.0 *F Content-MD5: ZaFAB849KNIKmFcXdu5eEg== *F X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ *F X-Cam-AntiVirus: No virus found *F X-Cam-SpamDetails: Not scanned *F Hi Bruce, *F How are you? Well I hope \- hope you don't mind this query. We send out *F 100 such every meeting, mostly asking a similar question... *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Basigin and integrin in Drosophila extra-embryonic membranes promote *F morphogenetic cell-cell interactions and prevent anoikis. *F You mention a gene symbol that is new to FlyBase, Basigin. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an annotation is *F named with a more descriptive or functional name. *F I notice that another abstract mentions a Basigin, by Kathy Curtin: *F Basigin, a metastatic factor in mammals, affects cellular architecture in *F Drosophila *F Kathy has also been asked for a CG number \- it'll all become clear eventually *F whether the two Basigins are in fact the same gene. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 09:53:25 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: reed@sickkids.ca *F Subject: Re: Helping FlyBase: ADRC-10784 *F Rachel, *F ... *F See below for details on what we are calling the Drosophila basigin gene *F (it's a well characterized glycoprotein in mammals that, among other *F things, is expressed at high levels on the surface of tumors and is thought *F to be associated with invasivness). *F ... *F Bruce *F >You mention a gene symbol that is new to FlyBase, Basigin. Do you know *F >which of the Genome Project CG annotations your gene corresponds to? *F Yes: CG31605 *F There is a male sterile reported to be a mutation in CG31605 called gelded. *F There is, however, some conflicting complementation data concerning the *F gelded locus and mutations in CG31605. These data indicate complex *F complementation, which is a likely scenerio given that CG31605 is *F predicted by Gadfly to produce nine different transcripts and two different *F proteins. We haven't really sorted out this complex complementation *F AS I mentioned above, we have a paper in press in Current Biology \- our *F supplementary information includes RT-PCR data showing that the PTT *F insertion G289 forms a hybrid RNA with CG31605. We have also shown that *F the enhancer trap line B39.1M2 is inserted in CG31605. # *U FBrf0178778 *a Kernan *b M. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:23:09 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10632 *F To: mkernan@notes.cc.sunysb.edu *F Cc: rd120@gen.cam.ac.uk *F Hi there Maurice, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A flagellar polycystin required for sperm storage and sperm competition. *F You mention a gene symbol that is new to FlyBase, Amo. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. *F .... *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Subject: Re: Helping FlyBase: ADRC-10632 *F To: 'Rachel Drysdale (Genetics)' *F From: mkernan@notes.cc.sunysb.edu *F Date: Mon, 23 Feb 2004 10:18:42 \-0500 *F Hi Rachel *F amo = 'almost there' = CG6504 = Pkd2. *F ... *F maurice *F ______________________________________ *F Maurice Kernan *F Associate Professor *F Department of Neurobiology & *F Center for Developmental Genetics *F SUNY at Stony Brook *F CMM447, Stony Brook, NY 11794-5140 *F (631)632-9964 (office) \-9182 (lab) \-6661 (fax) *F mkernan@notes.cc.sunysb.edu *F ______________________________________ # *U FBrf0178779 *a Stathopoulos *b A. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:16:03 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10030 *F To: angelike@uclink4.berkeley.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Angelike, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F FGF genes that pattern the mesoderm of Drosophila embryos. *F You mention two gene symbols that are new to FlyBase, pyr and ths *F (great names!). Do you know which of the Genome Project CG annotations *F your genes correspond to? All the CGs have corresponding gene records *F in FlyBase already and we don't like to make duplicate records for what *F is actually the same gene unless we can't avoid it. The CG symbol *F becomes a synonym when an annotation is named with a more descriptive or *F functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 07:16:21 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: Angela Stathopoulos *F Subject: Re: Helping FlyBase: ADRC-10030 *F Dear Rachel, *F pyr is CG13194 and ths is CG12443. *F If you need any more info, please let me know. When will this *F information become publically available? *F thanks, *F Angelike # *U FBrf0178780 *a Daga *b A. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:48:00 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10451 *F To: alessia.gazziero@unipd.it *F Cc: rd120@gen.cam.ac.uk *F Dear Alessia, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Molecular and functional analyses of the Drosophila atlastin homolog *F You mention a gene symbol that is new to FlyBase, atlastin. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if atlastin has picked up a short symbol since you submitted the *F abstract then this would be a good time to tell me so I can get it into *F the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 16:44:42 \+0100 *F To: rd120@gen.cam.ac.uk *F From: Andrea Daga *F Subject: Atlastin *F Dear Rachel, *F I am replying to your request on behalf of Alessia. The gene we mention in *F the abstract is the fly homolog of human SPG3a or atlastin. It corresponds *F to CG6668 and does not have an official name yet. We have named it *F D-atlastin and I would suggest using atl as a shorthand. *F Best wishes, *F Andrea *F Andrea Daga *F Assistant Telethon Scientist *F Dulbecco Telethon Institute and *F Department of Pharmacology *F Fondazione Telethon and *F University of Padova *F Largo Meneghetti 2 *F 35131 Padova \- Italy *F Tel: \+39-0498275778 *F Fax: \+39-0498275093 *F email: daga@unipd.it adaga@dti.telethon.it # *U FBrf0178781 *a Texada *b M. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:24:45 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10661 *F To: mtexada@rice.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Michael, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Yuri, a gene affecting Drosophila gravitaxis. *F You mention your gene, yuri. Could you tell me which of the Genome *F Project CG annotations your gene corresponds to? All the CGs have *F corresponding gene records in FlyBase already and we don't like to make *F duplicate records for what is actually the same gene unless we can't *F avoid it. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Michael Texada' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10661 *F Date: Mon, 23 Feb 2004 10:47:47 \-0600 *F hiya- *F it's 31732. *F take it easy- *F michael # *U FBrf0178782 *a Rothenfluh *b A. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:43:00 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10332 *F To: adrianr@itsa.ucsf.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Adrian, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F white rabbit affects behavioral responses to nicotine and alcohol *F You mention a gene symbol that is new to FlyBase, white-rabbit. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if white-rabbit has picked up a short symbol since you submitted *F the abstract then this would be a good time to tell me so I can get it *F into the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F To: rd120@gen.cam.ac.uk *F From: adrianr@itsa.ucsf.edu *F Date: Mon, 23 Feb 2004 13:57:48 PST *F Subject: Re: Helping FlyBase: ADRC-10332 *F Rachel *F the mutant white rabbit (whir) has previously been annotated as 5-131 *F (from the CSHL neurofly meeting). *F i am currently closing in on a paper, and will gladly let you know as *F soon as it's accepted about the CGs involved. *F .. *F let me know if that sound fine with you *F thanks for your understanding *F regard, adrian # *U FBrf0178783 *a Finn *b B. *t 2004.2.23 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:17:30 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10503 *F To: bkf4@duke.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Bridget, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Investigating the role of CDEP in regulation of apical-basal polarity and Rho *F pathway activity. *F You mention a gene symbol that is new to FlyBase, Cdep. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 17:58:35 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F From: Bridget Finn *F Subject: Re: Helping FlyBase: ADRC-10503 *F It's CG31536, I've been calling it CDEP after the human homologue. *F ... *F Bridget Finn *F bkf4@duke.edu # *U FBrf0178784 *a Takanaka *b Y. *t 2004.2.24 *T personal communication to FlyBase *u *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10154 *F To: ytakanak@ucla.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Yoko, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Roles for SUMO in Drosophila development. *F You mention a gene symbol that is new to FlyBase, sumo. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Yoko Takanaka' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10154 *F Date: Mon, 23 Feb 2004 16:33:26 \-0800 *F Dear Rachel, *F SUMO is also called as 'smt3'. *F Recently, SUMO is more popular than smt3. *F This is the flybase site of the smt3 gene. *F http://flybase.bio.indiana.edu/.bin/fbidq.html?FBan0004494 *F Sincerely, *F Yoko Takanaka *F Yoko TAKANAKA, Ph. D (ytakanaka@ucla.edu) *F Dept. of Chemistry and Biochemistry *F UCLA *F Young Hall, rm. 5044 *F 607 Charles E. Young Drive East *F Los Angeles, CA 90095-156905 *F phone: 310-206-4038 # *U FBrf0178785 *a Foley *b E. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:34:09 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10148 *F To: foley@itsa.ucsf.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Edan, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A Comprehensive RNAi Screen for Novel Innate Immune Regulators *F You mention a gene symbol that is new to FlyBase, Dnr1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 16:55:15 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: Edan Foley *F Subject: Re: Helping FlyBase: ADRC-10148 *F Hi Rachel, *F we identified Dnr1 in a screen for genes that regulate the innate *F immune response. The corresponding gene is CG 12489. *F best *F Edan *F \-- *F Edan Foley, Ph. D. *F 600 16th Street *F S374 Dept. of Biochemistry & Biophysics *F UCSF *F San Francisco *F CA. 94143-2200 *F Tel: \++ 415 4764709 *F Fax: \++ 415 5144080 *F email: foley@itsa.ucsf.edu *F URL: http://www.ucsf.edu/poflab/ # *U FBrf0178786 *a Burke *b R. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:42:40 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10326 *F To: burker@unimelb.edu.au *F Cc: rd120@gen.cam.ac.uk *F Dear Richard, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of dmATP7, the Drosophila homologue of the mammalian Menkes and *F Wilsons Disease copper-transporting P-type ATPases, in Drosophila S2 cells *F You mention a gene symbol that is new to FlyBase, ATP7. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Tue, 24 Feb 2004 13:53:19 \+1100 *F From: Richard Burke *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10326 *F Dear Rachel, *F The CG number for the gene I mention, DmATP7, is CG1886, *F All the best, *F Richard Burke *F 'Rachel Drysdale (Genetics)' wrote: # *U FBrf0178787 *a Zid *b B. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:43:24 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10336 *F To: zid@caltech.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Brian, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Polyamines and the aging process in Drosophila. *F You mention a mutant \- DJ709 \- with an insertion into the transcription *F unit represented by genome annotation CG8327. The insertion is of an *F enhancer trap element \- could you tell me which one? That way I can *F record the allele details correctly from teh start. In view of the *F work that you have done on CG8327 I'm wondering whether you have named *F it with a more descriptive name. If so then this would be a good time *F to tell me so I can get it into the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 23 Feb 2004 20:01:39 \-0800 (PST) *F From: Brian Zid *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10336 *F Rachel, *F DJ709 is a pGawB line. The line is part of a collection created by *F Laurent Seroude, a former post-doc from the Benzer lab who is now a *F professor at Queens College in Canada. A paper which describes the *F collection of lines Laurent generated is 'Seroude, L. Gal4 Drivers *F Expression in the Whole Adult Fly (2002)Genesis 34: 34-38'. Though this *F paper does not specifically mention DJ709. *F For the name of the gene I feel that SpdS, spermidine synthase, is the *F most descriptive, instead of trying to give a name related to lifespan *F extension. *F Hope this helps you out, and if I can be of any more help feel free to *F ask. *F Brian M. Zid *F Division of Biology *F Caltech 156-29 *F 1200 E. California Blvd *F Pasadena CA 91125 *F (626)395-2186 # *U FBrf0178788 *a Fayazi *b Z. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:27:39 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10840 *F To: zsfayazi@buffalo.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Zahra, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The effect of a Drosophila ortholog of the human MRJ on polyglutamine toxicity *F and aggregation. *F You mention a gene symbol that is new to FlyBase, mrj. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Tue, 24 Feb 2004 09:17:44 \-0500 *F From: zsfayazi@acsu.buffalo.edu *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10840 *F Dear Rachel *F Hi. I am writting regarding my abstract: The effect of a Drosophila *F ortholog of the human MRJ on polyglutamine toxicity and aggregation, for *F the upcoming 45th Annual Drosophila Research Conference. The gene symbol *F mrj is correspond to CG8448 in FlyBase. *F Best regards *F Zahra *F Zahra Fayazi DVM., PhD *F Posdoctoral Research Scholar *F Biomedical Research Building, Room 520 *F 3435 Main Street, 124 Sherman Hall *F Buffalo, N.Y., 14214 *F Phone: 716 \- 829 \- 3050 *F Fax 716 \- 829 \- 3050 *F Email zsfayazi@buffalo.edu # *U FBrf0178789 *a Benson *b E. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:33:15 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10142 *F To: elizabeth.benson@zoology.ox.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Elizabeth, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Epigenetic Regulation by the aly-class Meiotic Arrest Genes *F You mention gene symbols that are new to FlyBase, schumacher-levy and *F hale-bopp. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. Also, since the names for your genes are too long to *F use as symbols now would would be a good time to tell me what short *F symbols you would like to use so I can get them into the database from *F the start. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: Elizabeth Benson *F Date: Tue, 24 Feb 2004 14:40:38 \+0000 (GMT) *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10142 *F Dear Rachel, *F Thanks for your email. The CG numbers are CG7570 for hale-bopp which we *F want to shorten to hale, and CG17736 for shumacher-levy, which we want to *F shorten to schuy. We gave these names for these genes because in their testes *F in-situs they gave a pattern on the elongating spermatids which looked liked *F comet tails. *F I hope this helps, Elizabeth # *U FBrf0178790 *a Sava *b E. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:46:58 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10411 *F To: elladasav@hotmail.com *F Cc: rd120@gen.cam.ac.uk *F Dear Ellada, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterisation of the Drosophila CAP-D2 condensin subunit during the cell *F cycle and its role in chromosome dynamics during mitosis *F You discuss the CAP-D2 gene. We believe this to be represented by *F annotation CG1911. Could you confirm this for me please, or tell me *F which other CG annotation it corresponds to. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'elli sava' *F To: rd120@gen.cam.ac.uk *F Subject: RE: Helping FlyBase: ADRC-10411 *F Date: Tue, 24 Feb 2004 21:03:21 \+0200 *F Helo Rachel, *F The CAP-D2 gene is indeed the CG1911 in Drosophila melanogaster. *F Best wishes *F Ellada # *U FBrf0178791 *a Preat *b T. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:18:11 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10536 *F To: preat@iaf.cnrs-gif.fr *F Cc: rd120@gen.cam.ac.uk *F Dear Thomas, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Cysteine proteinase pathway required for long-term memory formation *F You mention a gene symbol that is new to FlyBase, cer. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Wed, 25 Feb 2004 09:54:49 \+0100 *F Subject: Re: Helping FlyBase: ADRC-10536 *F From: Thomas PREAT *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F crammer corresponds to CG10460. *F Best wishes, *F Thomas. # *U FBrf0178792 *a Harte *b P. *t 2004.2.24 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:31:16 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10072 *F To: rlk6@cwru.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Rebeccah, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of Esc-like, an ESC paralog *F You mention a gene symbol that is new to FlyBase, escl. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Tue, 24 Feb 2004 10:37:48 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Peter Harte *F Subject: Re: FW: Helping FlyBase: ADRC-10072 *F Hi Rachel, *F Rebeccah passed your request on to me. We found 'esc-like' (CG5202) *F the week the genome sequence was released based on its high degree of *F protein sequence similarity to ESC. We raised antibodies to it and *F subsequently named it esc-like (escl) based not only on it high *F amino acid sequence similarity to ESC, but also based on our *F discovery that the ESCL protein is present in a complex in vivo with *F the same proteins that are also found in the ESC-E(Z) complex (namely *F E(Z), SU(Z)12, p55/Caf1-p55, RPD and PCL). The ESCL complex appears *F to be an 'alternative' ESC complex and we have functional evidence *F that it is involved in PcG silencing. It also co-localizes with these *F E(Z), SU(Z)12 and PCL at many sites on polytene chromosomes. This *F work will be reported soon. Related papers from our lab on ESC are *F below. *F Peter Harte *F Tie, F., Furuyama, T. and Harte, P.J. (2001) The Drosophila Polycomb *F Group proteins ESC and E(Z) are present in a complex containing the *F histone-binding protein p55 and the histone deacetylase RPD3. *F Development 128:275-86. *F Furuyama, T., Tie, F. and Harte P.J. (2003) Polycomb group *F proteins ESC and E(Z) are present in multiple distinct complexes that *F undergo dynamic changes during development. Genesis 5:114-24. *F Tie, F., Prasad-Sinha, J., Birve, A., Rasmuson-Lestander, Å. and *F Harte, P.J. (2003) A 1 MDa ESC/E(Z) complex from Drosophila that *F contains the Polycomblike, and RPD3 proteins. Mol. Cell. Biol. 23: *F 3352-62 *F \-- *F Peter Harte *F Dept. of Genetics *F School of Medicine *F Case Western Reserve University *F 10900 Euclid Ave.(BUT FOR COURIER SERVICES USE: 2109 Adelbert Rd.) *F Cleveland, OH 44106-4955 *F phone: (216) 368-6417 *F fax: (216) 368-3432 *F e-mail: pjh3@po.cwru.edu # *U FBrf0178793 *a Qian *b L. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:28:44 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10898 *F To: qianl@umich.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Li, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The function of T-box neuromancer genes in Drosophila heart development *F You mention two gene symbols that are new to FlyBase, neuromancer1 and *F neuromancer2. Do you know which of the Genome Project CG annotations *F your genes correspond to? All the CGs have corresponding gene records *F in FlyBase already and we don't like to make duplicate records for what *F is actually the same gene unless we can't avoid it. FlyBase already *F has a gene record, for H15 (FBgn0016660), a gene which has also been *F referred to by the name neuromancer, and encodes a T-box domain *F protein. What is the relationship of neuromancer1 and neuromancer2 to *F H15? Finally, if you have come up with short symbols for neuromancer1 *F and neuromancer2 now would be a good time to tell me \- so that I can *F them into the database from the start. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Li Qian' *F To: ''Rachel Drysdale \(Genetics\)'' *F Subject: RE: Helping FlyBase: ADRC-10898 *F Date: Tue, 24 Feb 2004 20:22:59 \-0800 *F Dear Rachel *F I am sorry for the confusion. Neuromancer1 actually is neuromancer (H15, *F short symbol for it is nmr). Recently we identified another T-box gene *F (CG6634) that is highly conserved with nmr1 and positional very close to *F it, meanwhile some of our preliminary data suggest that CG6634 might *F also function redundantly as nmr1, so we renamed it as neuromancer2 *F (nmr2). Nmr1 and nmr2 are likely to have evolved by recent gene *F duplication, that's why we use the same name but different numbers to *F distinguish these two genes. *F If you have any further questions, please feel free to contact me. *F Bests, Li # *U FBrf0178794 *a Guerrero *b I. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:30:13 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10918 *F To: iguerrero@cbm.uam.es *F Cc: rd120@gen.cam.ac.uk *F Dear Isabel, *F .. *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Shifted, the Drosophila ortholog of the vertebrate Wnt inhibitory factor (WIF) *F is required for Hedgehog transport. *F Your abstract indicates that you know which CG corresponds to shf *F (FBgn0003390). Could you let me inow which CG this is please? All the *F CGs have corresponding gene records in FlyBase already and we don't *F like to make duplicate records for what is actually the same gene *F unless we can't avoid it. If you let us know then I can merge the gene *F records. The CG designation would become a synonym and shf would *F become the valid symbol. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F To: rd120@gen.cam.ac.uk *F From: iguerrero@cbm.uam.es *F Subject: shifted *F Date: Wed, 25 Feb 2004 11:06:41 GMT *F Dear Rachel, *F .. *F Shifted or D-WIF corresponds to CG3135 (FBgn0029901). *F .. *F With best wishes, *F Isabel # *U FBrf0178795 *a Yang *b H.P. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:42:24 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10321 *F To: hpyang@ym.edu.tw *F Cc: rd120@gen.cam.ac.uk *F Dear Hsiao-Pei, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F hydra, a recently evolved gene in Drosophila *F You mention a gene symbol that is new to FlyBase, hydra. Do you know *F which of the Genome Project CG annotations your gene corresponds to? I *F realise that given the struture of the genome where it is located there *F may not be a CG, but I have to ask. All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F The CG symbols become synonyms when an annotation is named with a more *F descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Hsiao-Pei Yang' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: RE: Helping FlyBase: ADRC-10321 *F Date: Wed, 25 Feb 2004 20:12:01 \+0800 *F >You mention a gene symbol that is new to FlyBase, hydra. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F Hi Rachel, *F 'hydra' is CG1338 on X chromosome. *F Best, *F Hsiao-Pei *F Hsiao-Pei Yang, Ph.D. *F Assistant Professor *F Institute of Genetics *F National Yang Ming University *F Taipei, Taiwan *F TEL:886-22826-7039 *F FAX:886-22826-4930 *F Email: hpyang@ym.edu.tw # *U FBrf0178796 *a Oikemus *b S. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:25:27 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10720 *F To: Sarah.Oikemus@umassmed.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Sarah, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Drosophila homolog of the Atm DNA damage response protein is required for *F HP1 and HOAP localization to telomeres. *F You discuss the atm/tefu gene. Could you tell me which of the Genome *F Project CG annotations your gene corresponds to? All the CGs have *F corresponding gene records in FlyBase already and we don't like to make *F duplicate records for what is actually the same gene unless we can't *F avoid it. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Subject: RE: Helping FlyBase: ADRC-10720 *F Date: Wed, 25 Feb 2004 09:44:49 \-0500 *F From: 'Oikemus, Sarah' *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F The atm/tefu gene corresponds to CG6535. *F Sarah # *U FBrf0178797 *a Munoz *b S. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:29:46 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10094 *F To: silvia.munoz@uv.es *F Cc: rd120@gen.cam.ac.uk *F Dear Silvia, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F cabut encodes a C2H2 zinc finger transcription factor required during *F Drosophila embryogenesis *F You mention a gene symbol that is new to FlyBase, cabut. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Wed, 25 Feb 2004 17:01:07 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Silvia Muñoz *F Subject: Re: Helping FlyBase: ADRC-10094 *F Dear Rachel, *F cabut corresponds to the annotation CG4427 of the Flybase. *F Silvia *F Silvia Muñoz Descalzo *F Dept. de Genética (Fac. de CC. Biológicas, UV.) *F Avd. Dr. Moliner nº 50 *F CP 46100 Burjassot *F Tel. 96-354.43.79 *F Fax 96-354.30.29 *F silvia.munoz@uv.es # *U FBrf0178798 *a Lee *b S. *t 2004.2.26 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:25:04 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10673 *F To: sangil.lee@yale.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Sangil, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Oocyte integrity during rapid cytoplasm transport requires jagunal. *F You mention a gene symbol that is new to FlyBase, jagn. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Wed, 25 Feb 2004 22:24:00 \-0500 *F From: sangil.lee@yale.edu *F To: 'Rachel Drysdale (Genetics)' *F Cc: sangil.lee@yale.edu, rd120@gen.cam.ac.uk, lynn.cooley@yale.edu *F Subject: Re: Helping FlyBase: ADRC-10673 *F Dear Rachel, *F The gene jagunal (jagn) corresponds to CG10978. Germ line clone *F phenotype of the jagn mutation is small egg. So we named the gene *F jagunal. jagunal means small egg in Korean. It will be great that *F CG10978 is named jagunal (jagn). *F Best wishes, *F Sangil # *U FBrf0178799 *a McNabb *b S. *t 2004.2.26 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:39:46 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10237 *F To: smcnabb@u.washington.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Sue, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Spread your wings and tan with bursicon. *F We will rename CG13419 to be bursicon, and CG13419 will be relegated to *F synonymy. I was wondering if you have settled on a short symbol for *F bursicon. If so then this would be a good time to tell me so I can *F get it into the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Thu, 26 Feb 2004 11:14:38 \-0800 *F Subject: Re: Helping FlyBase: ADRC-10237 *F From: Susan McNabb *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F My collaborators and I think your proposed renaming will be grand. Our short *F symbol is burs. Not so short, but bu and bur are already taken and we have *F used burs in our paper which has been accepted by Current Biology . *F ... *F Best wishes, *F Sue *F \-- *F Susan L. McNabb, Research Asst Prof *F Department of Biology, Box 351800 *F University of Washington *F Seattle, WA 98195-1800 *F tel: (206)543-6513 *F fax: (206)616-2011 *F email: smcnabb@u.washington.edu # *U FBrf0178800 *a Gruntenko *b N. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:38:35 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10215 *F To: nataly@bionet.nsc.ru *F Cc: rd120@gen.cam.ac.uk *F Dear Natalia, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Juvenile hormone and 20-hydroxyecdysone in Drosophila oogenesis under heat *F stress. *F You mention a mutant that is new to FlyBase, Dvir\hs. Can you give me *F any information about its map location \- this is a data class we like *F to track, if possible. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Nataly Gruntenko' *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10215 *F Date: Fri, 27 Feb 2004 12:54:23 \+0600 *F Dear Rachel, *F hs mutation of D. virilis prevents the development of heat stress response *F in JH metabolic system in larvae and adults. It is located in chromosome 6. *F Unfortunately, we had not determine its map location since we didn't have *F strains with visible muations in chromosome 6. *F Sincerely, *F Nataly # *U FBrf0178801 *a Kulikova *b D. *t 2004.2.27 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:41:05 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10271 *F To: dinakulikova@excite.com *F Cc: rd120@gen.cam.ac.uk *F Dear Dina, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F ANALYSIS OF TOOTHRIN LOCUS IN DROSOPHILA *F You mention a gene symbol that is new to FlyBase, tth. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. The CG symbols become synonyms when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F To: rd120@gen.cam.ac.uk *F Subject: RE: Helping FlyBase: ADRC-10271 *F From: 'Dina' *F Date: Fri, 27 Feb 2004 13:26:49 \-0500 (EST) *F Dear Rachel, *F We are very sorry for incomplete information, concerning toothrin gene *F published in abstract. We are hurry to improve the situation. So, this locus *F corresponds to 'CG12175'. *F Sincerely, Dina. # *U FBrf0178802 *a Mecklenburg *b K. *t 2004.3.1 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:23:33 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10641 *F To: kmecklen@iusb.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Kirk, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A novel Drosophila retina gene with MORN repeats is conserved in Humans. *F You mention a gene symbol that is new to FlyBase, 9C4. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. *F Also, if 9C4 has picked up a more descriptive name/symbol since you *F submitted the abstract then this would be a good time to tell me so I *F can get it into the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Subject: RE: Helping FlyBase: ADRC-10641 *F Date: Mon, 1 Mar 2004 10:18:37 \-0500 *F From: 'Mecklenburg, Kirk L.' *F To: 'Rachel Drysdale (Genetics)' *F Hi back! *F Sorry its taken me so long to respond. *F I was a little worried that by giving the gene symbol someone might scoop me. *F I guess I've already outed myself though, so.... *F 9C4 corresponds to CG10233. *F Its from the reference: *F Hyde, D., Mecklenburg, K., Pollock, J., Vihtelic, T., and Benzer, S. (1990). *F Twenty Drosophilia visual system cDNA clones: One is a homolog of human *F arrestin. PNAS 87, 1008-1012. *F Thanks for your help. *F Kirk Mecklenburg *F Assoc. Prof. Genetics *F Indiana University South Bend # *U FBrf0178803 *a Roote *b J. *t 2004.3.1 *T personal communication to FlyBase *u *F To: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: In(2L)C163.41 *F Date: Mon, 1 Mar 2004 11:49:44 \+0000 *F Dear Rachel, *F 2 things: *F 1) please could I correct the genetic data that I gave you for the *F aberration In(2L)TE35B-210L C163.41R . It actually disrupts noc \-- *F beat-Ia. *F 2) The Fambrough and Goodman paper states that this aberration breaks *F within beat-Ia. Can (should) this data also be included in the *F In(2L)C163.41 record (FBab0004400), which is of course the same *F breakpoint? *F Thank you. *F John # *U FBrf0178804 *a Popkova *b A. *t 2004.2.27 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:15:07 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10004 *F To: popkova@img.ras.ru *F Dear Anna, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Variable effects of the glass-like mutation on the mini-white *F expression depend on the site of transgene insertion. *F You mention a gene symbol that is new to FlyBase, gl-l. Do you know *F yet which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbol becomes a synonym when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Fri, 27 Feb 2004 16:54:25 \+0300 *F From: Popkova *F To: rd120@gen.cam.ac.uk *F Subject: gl-l *F Dear Rachel, *F I don`t know which of Genome Project CG annotations glass-like (gl-l) locus *F corresponds to. *F gl-l is new mutation obtained by X-irradiation. *F At the present time I succeed only in recombination deletion mapping. *F Recombination mapping localized the gl-l gene in position 1-28,4. *F Using deficiencies Df(1)KA14, Df(1)C52, Df(1)M38-C5, Df(1)18.1.15 *F from Bloomington collection and *F Df(1)ED6957 obtained from Dr. H. Stocker gl-l was mapped to region 8C13-8E. *F I fail to map gl-l gene more exactly. *F With best wishes, *F Anna *F Date: Thu, 4 Mar 2004 12:02:40 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: gl-l *F To: rd120@gen.cam.ac.uk, popkova@img.ras.ru *F Dear Anna, *F Many thanks for your helpful reply, which I will curate as a *F personal communication from you to FlyBase. *F Please could you confirm my interpretation of your deletion mapping *F results .... from what you say I understand that *F Df(1)KA14 does not delete gl-l *F Df(1)C52 does not delete gl-l *F Df(1)ED6957 does not delete gl-l *F Df(1)M38-C5 does delete gl-l *F Df(1)18.1.15 does delete gl-l *F do I have that correct? *F With best wishes, *F Rachel. *F Date: Fri, 5 Mar 2004 12:11:19 \+0300 *F From: Popkova *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re2: gl-l *F Dear Rachel, *F Thanks for your attention to my work *F You interpretation of gl-l deletion mapping results is correct *F With best wishes, *F Anna # *U FBrf0178805 *a Dockendorff *b T. *t 2004.3.4 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:32:04 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10112 *F To: dockentc@muohio.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Thomas, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A Drosophila orthologue of PICK1 interacts with Germcell-less during pole cell *F development *F You mention a gene symbol that is new to FlyBase, PICK1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Thu, 4 Mar 2004 09:16:55 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F From: Tom Dockendorff *F Subject: Re: Helping FlyBase: ADRC-10112 *F Rachel, *F The Drosophila PICK1 orthologue corresponds to CG6167. It has about *F 60% amino acid identity with the mouse PICK1 gene, hence referring to *F the fly gene as PICK1. *F Sincerely, *F Tom Dockendorff *F \-- *F Dr. Tom Dockendorff *F Dept. of Zoology *F Miami University *F 212 Pearson Hall *F 500 E. High Street *F Oxford, OH 45056 *F Ph. 513-529-3126 *F FAX 513-529-6900 # *U FBrf0178806 *a Krieser *b R. *t 2004.3.4 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:47:14 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10443 *F To: ron.krieser@cbrc2.mgh.harvard.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Ronald, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A screen for genes involved in the phagocytosis of apoptotic cells. *F You mention a gene symbol that is new to FlyBase, PSR. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: 04 Mar 2004 10:04:20 \-0500 *F From: Ron Krieser *F Subject: Re: Helping FlyBase: ADRC-10443 *F To: Rachel Drysdale *F Hello Rachel, *F Sorry I did not respond sooner. The gene that I am referring to is CG5383. *F This gene is similar to the human phosphatidylserine receptor and codes for *F the following amino acid sequence: *F MSEEFKLPKRSRKRTREVKRKARPELDGENAWSAMRYCEKFEPFWDFTDN *F LERIEESQVPESEFIERFERPYKPVVIRGCTDGWLALEKWTLARLAKKYR *F NQKFKCGEDNEGYSVKMKMKYYVEYMQSTRDDSPLYIFDSSFGEHHRRRK *F LLDDYVVPKYFRDDLFQYCGENRRPPYRWFVMGPARSGTGIHIDPLGTSA *F WNTLIRGHKRWCLFPTQTPKELLKVTSAMGGKQRDEAITWFSTIYPRTQL *F PSWPEQYRPIEVLQGAGETVFVPGGWWHVVLNMDDTIAITQNFSSQTNFP *F CVWHKTVRGRPKLSRKWLRVLRDQRPELAQIADSINLNESTGFASDSSSN *F SSSSSSSSSSSSEEEESDDGGDSNTDSGQESLTAKKKKKRRMAGGGSGSG *F SMGGSSRS *F I hope this helps *F Ron Krieser # *U FBrf0178807 *a Rikhy *b R. *t 2004.3.5 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:15:44 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10025 *F To: richar@tifr.res.in *F Cc: rd120@gen.cam.ac.uk *F Dear Richa, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of Dynamin related protein in synaptic function in Drosophila. *F You 'report the genetic and physiological characterization of *F a Dynamin related protein at the Drosophila neuromuscular junction', *F but do not name it. If you can let us know which CG this gene *F corresponds to we can record your work on it by listing your abstract *F as a reference for the gene, and if you wish to confer a functional or *F phenotypic name then now would be a good time to do that. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Fri, 5 Mar 2004 10:13:54 \+0530 (IST) *F From: Richa Vinod Rikhy *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10025 *F Dear Rachel, *F I apologise for the delay in reply. *F This study is associated with the gene CG3210. *F We refer to the mutants obtained as 'noodle'. *F Thanks a lot for your email. *F Regards, *F Richa Rikhy # *U FBrf0178808 *a Klinge *b K. *t 2004.2.25 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:32:30 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10124 *F To: kklinge@gwdg.de *F Cc: rd120@gen.cam.ac.uk *F Dear Kathrin, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Ballchen is a novel Ser / Thr Kinase involved in germ cell development. *F You mention a gene symbol that is new to FlyBase, ballchen. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, you mention an EP insertion \- is this one of the Gene Disruption *F Project elements? If so we probably already have a record of that *F insertion, so it would be good if you let me know its identifier number *F and we can keep the insertion properly associated with the ballchen allele. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Fri, 5 Mar 2004 11:32:59 \+0100 *F To: rd120@gen.cam.ac.uk *F From: Kathrin Klinge *F Dear Rachel, *F the corresponding CG number is 6386. The gene is named baellchen (with *F an 'Umlaut'). The identifier number of the EP insertion that I *F mentioned ['EP-Element insertion representing a hypomorphic mutation of *F ballchen showed a male sterile phenotype with reduced testis'] is *F EP863. *F With best regards, *F Kathrin *F Kathrin Klinge *F Max Planck-Institut für biophysikalische Chemie *F Abteilung Molekulare Entwicklungsbiologie (170) *F Am Fassberg 11 *F 37077 Göttingen *F Tel.: 0551-201-1657 *F Fax: 0551-201-1755 *F e-mail: kklinge@gwdg.de # *U FBrf0178809 *a Grammenoudi *b S. *t 2004.3.5 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:44:35 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10357 *F To: grammenoudi@fleming.gr *F Cc: rd120@gen.cam.ac.uk *F Dear Sofia, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Identification and characterization of DSNX6, a sorting nexin that interacts *F with 14-3-3. *F You mention a gene symbol that is new to FlyBase, Dsnx6. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F What does the D in the symbol stand for? Gene symbols should not be *F prefixed with D for Drosophila (see nomenclature posting below) ... *F should the valid gene symbol be Snx6 for 'Sorting nexin 6'? *F With best wishes, *F Rachel. *F >From: kaufman@bio.indiana.edu (T. Kaufman) *F Newsgroups: bionet.drosophila *F Subject: Nomenclature *F Date: 17 Mar 1999 08:24:52 \-0800 *F Organization: BIOSCI International Newsgroups for Molecular Biology *F Distribution: world *F Message-ID: *F NNTP-Posting-Host: net.bio.net *F Xref: news.indiana.edu bionet.drosophila:4157 *F ============= *F Dear Colleagues, *F We are writing with a suggestion about the nomenclature of Drosophila *F genes. *F It is now routine that a D. melanogaster gene is identified on the *F basis of sequence homology with (or more rarely, functional *F complementation for) a gene from another organism such as a yeast or a *F vertebrate. Authors often prefix these Drosophila versions with 'D' or 'd' *F for Drosophila, or 'Dm' for D. melanogaster. However in the absence of a *F unifying pan-biological system of genetic nomenclature, to haphazardly *F use 'D' or 'd' or 'Dm' is actually counterproductive. Think of Danio, *F Dictyostelium, Daphnia magna .... there is room for confusion. *F FlyBase uses a defined system of prefixes for denoting species other *F than D. melanogaster. Examples are 'Dvir\' for D. virilis, 'Dsim\' for D. *F simulans, etc. Examples of gene symbols are Dvir\Adh1 and Dsim\per *F (see flybase/Documents/nomenclature/species-abbreviations.txt for full *F listing of species abbreviations). We have not explicitly listed *F melanogaster genes with the prefix 'Dmel\' since we consider that to be *F implicit. However we would like to suggest that when meaning to *F distinguish between a vertebrate gene and its Drosophila version that *F would otherwise have the same symbol, authors prefix the symbol with *F 'Dmel\'. 'D', 'd', or 'Dm' ought NOT to be used as a prefix to a *F D. melanogaster gene symbol \- this is a long-standing convention, having *F been made explicit by Lindsley and Zimm in the 1992 edition of the Red *F Book. *F FlyBase *F ============ *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'grammenoudi' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: ADRC-10357 *F Date: Fri, 5 Mar 2004 18:22:10 \+0200 *F Hello, *F I am very sorry for the delay and I hope that I didnt cause any problem. *F Indeed the D in DSNX6 stands for Drosophila. *F The CG number of the protein in the flybase is CG8282 and it appears to be *F the homologue of sorting nexin 6. *F Sofia Grammenoudi # *U FBrf0178810 *a Davis *b M. *t 2004.3.5 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:40:08 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10241 *F To: davism04@med.nyu.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Marie, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The role of vretano in oogenesis *F You mention a gene symbol that is new to FlyBase, vret. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also \- we like to track the etymology of interesting gene symbols \- *F where does the vretano name come from? *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Fri, 05 Mar 2004 13:59:19 \-0500 *F From: Marie Y Davis *F Subject: Re: Helping FlyBase: ADRC-10241 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F We are currently cloning vreteno so I cannot yet give you a CG annotation. *F Vreteno means spindle in bulgarian, and the weak vreteno alleles give a *F spindle phenotype. This name was given by a former graduate student who *F recovered this gene from our 3R maternal effect screen. We hope to identify *F the CG annotation soon! \-Marie # *U FBrf0178811 *a Page *b S. *t 2004.3.5 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:41:45 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10294 *F To: slp@stowers-institute.org *F Cc: rd120@gen.cam.ac.uk *F Dear Scott, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A role for Kinesin-like protein 3A (Klp3A) in regulating meiotic exchanges is *F implicated by identification of the mei-352 mutation. *F At FlyBase we would merge the gene record for Klp3A with that for *F mei-352 if we were confident that the two records represent the same gene in *F reality. You wrote \- 'These results strongly indicate that klp3A is *F the gene responsible for the mei-352 mutant' \- and I'm wondering if by *F now the last vestige of reservation is gone and we can go ahead and *F merge these records. The evidence in the abstract looks promising ... *F The new name for the merged gene record would be Klp3A with mei-352 *F relegated to a synonym. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Subject: RE: Helping FlyBase: ADRC-10294 *F Date: Fri, 5 Mar 2004 16:55:18 \-0600 *F From: 'Page, Scott' *F To: 'Rachel Drysdale (Genetics)' *F Rachel, *F I am confident that mei-352 is an allele of Klp3A. Klp3A alleles 521 and *F 63e4 fail to complement mei-352 (other alleles have not yet been *F tested), and mei-352 bears a missense point mutation in a conserved *F residue of Klp3A. *F Sorry for the delay, *F Scott *F \++++++++++++++++++++++++++++++++++++++ *F Scott L. Page, Ph.D. *F Stowers Institute for Medical Research *F 1000 E. 50th Street *F Kansas City, MO 64110 *F (816) 926-4146 (lab) *F (816) 926-2057 (fax) *F slp@stowers-institute.org *F www.stowers-institute.org # *U FBrf0178812 *a Kiger *b J. *t 2004.3.4 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:40:26 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10245 *F To: jakiger@ucdavis.edu *F Cc: rd120@gen.cam.ac.uk *F Dear John, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F An epithelial mesenchymal transition precedes migration of cells from the wing *F during wing maturation. *F You discuss a gene, mapping to 93B-E, for which you have EMS alleles. *F Does this gene have a symbol:name yet? If so now would be a good time *F for me to get it into the database. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Thu, 4 Mar 2004 09:58:03 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: John Kiger *F Subject: Re: Helping FlyBase: ADRC-10245 *F Dear Rachel, *F Sorry to be so slow, but we just found an error in the location of *F this gene and I was waiting for some crosses to come out to confirm *F the true location. The gene is located between 93D1 \- 93F6-8 in *F Df(3R)e-H4. Several alleles were in the Zuker lab collection; we call *F it 3-5569 after one of the alleles. The name 'furled wing' \- flw has *F been suggested. Do you like it? *F Regards, *F John *F John A. Kiger, Jr. *F Professor of Genetics *F Section of Molecular & Cellular Biology *F University of California *F One Shields Avenue *F Davis, CA 95616 *F TEL 530-752-8204 *F FAX 530-752-3085 *F jakiger@ucdavis.edu *F Date: Mon, 8 Mar 2004 14:40:25 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10245 *F To: rd120@gen.cam.ac.uk, jakiger@ucdavis.edu *F Dear John, *F thanks for getting back to me \- I will curate the data as a *F personal communication from you to FlyBase. 'furled wing' is fine as a *F name but flw is already being used as a symbol for 'flap wing' *F (FBgn0000711), so you'll have to choose another abbreviation. frl seems *F to be free, as does fwi. I'm happy to check others for you, if you *F have another favorite. Let me know which you favor. *F All the best, *F Rachel. *F Date: Mon, 8 Mar 2004 10:13:00 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: John Kiger *F Subject: Re: Helping FlyBase: ADRC-10245 *F Dear Rachel, *F Mel says frl is fine as an abbreviation for 'furled wing'. Thank you *F for suggesting it. *F John # *U FBrf0178813 *a Sharma *b Y. *t 2004.3.5 *T personal communication to FlyBase *u *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Fri, 5 Mar 2004 17:08:57 \+0000 *F Mime-Version: 1.0 *F X-Sender: ysharma@aretha.jax.org *F Date: Fri, 5 Mar 2004 12:08:02 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Yashoda Sharma *F Subject: Flybase update *F X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ *F X-Cam-AntiVirus: No virus found *F X-Cam-SpamDetails: scanned, SpamAssassin (score=0.2, MIME_MISSING_BOUNDARY 0.16) *F Hello Rachel, *F About a year ago you contacted me about CG15148, which I asked you *F to rename Dhc36D, due to its sequence properties. I am writing to *F give you an update of this gene. My thesis work at the University of *F Iowa, with Dr. Daniel Eberl has revealed that Dhc36D is the *F previously identified audition gene beethoven. I've attached a *F synopsis of my findings. We would appreciate it very much if you *F could include this as a communication to FlyBase and update the *F information. *F If you have any questions please contact me at this email. Please *F let me know the status of this. *F thank you for your assistance in this matter *F Yashoda *F PCR, Southern analysis, and sequencing data indicate that the audition gene, *F beethoven (btv) is encoded by the Drosophila DHC1b isoform, Dhc36D. As the 1b *F isoform, btv appears to serve as the retrograde motor for intraflagellar *F transport specifically in the sensory cilia of Johnston's organ. This finding *F is supported by EM studies that show that the Johnston's organ cilia are *F disrupted (Eberl et al., 1997), similarly to DHC1b mutant cilia from other *F organisms, especially those of C. elegans (Signor et al., 1999). The *F defective btv cilia resemble those in mutants for the other recently *F identified Drosophila IFT components (Han et al., 2003; Sarpal et al., 2003). *F btv mutants are fertile, confirming the findings of Han and Sarpal that IFT is *F not required for Drosophila sperm development. *F The lesion in the btv5P1 allele (EMS-induced) is a 401 basepair deletion *F and a 6 basepair insertion. This likely eliminates one of the P-loop motifs *F required for ATP hydrolysis, or it may result in the production of a truncated *F protein. *F The other btv allele, k07109b is likely a 'hit and run' lesion, which we have *F not yet defined molecularly, but is NOT associated with the k07109b insertion *F of the PlacW element, which is actually present in the FasIII gene nearby. # *U FBrf0178814 *a Levis *b R. *t 2004.3.8 *T personal communication to FlyBase *u *F Date: Mon, 8 Mar 2004 11:52:05 \-0500 *F To: flybase-help@morgan.harvard.edu, kcook@bio.indiana.edu *F From: Robert Levis *F Subject: EP2387 is an allele of CG30055 *F The P-element insertion P{EP}Sin3AEP2387 (aka EP2387, FBti0016418) *F is currently annotated as an allele of Sin3A. I noticed that it is *F also inserted within the R3.1 annotated limits of CG30055, which is *F nested in an intron of Sin3A. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0178815 *a Bloomington Drosophila Stock Center *b ?. *t 2004.3.9 *T personal communication to FlyBase *u *F Date: Tue, 09 Mar 2004 16:11:31 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{hs-hid} insertions *F P{hs-hid}2 and P{hs-hid}3 are second and third chromosome insertions, *F respectively. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178816 *a Parks *b A. *t 2004.3 *T personal communication to FlyBase *u *F Date: Tue, 09 Mar 2004 16:47:27 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: XPG-L *F The following information from Annette Parks accompanied stocks donated to *F the Bloomington Stock Center by Exelixis, Inc. (3/04). *F The lethal insertion described in Parks et al. (Nature Genetics 36: 288 \- *F 292, 2004) as 'XPG-L' is an X chromosome insertion of P{XP}. It was *F isolated from transposition crosses described in Thibault et al. (Nature *F Genetics 36: 283 \- 287, 2004). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178817 *a Levis *b R. *t 2004.3.9 *T personal communication to FlyBase *u *F Date: Tue, 9 Mar 2004 12:21:38 \-0500 *F To: flybase-help@morgan.harvard.edu, kcook@bio.indiana.edu *F From: Robert Levis *F Subject: EP2509 is an allele of EcR *F EP2509 (aka P{EP}CG8347EP2509 FBti0011194) is currently annotated *F in FlyBase and the Bloomington Stock database as an allele of CG8347. *F CG8347 is an 'existence uncertain gene' that is not annotated as a *F gene in Release 3. Based on the flanking sequence in GenBank, EP2509 *F is inserted in the EcR gene (CG1765) according to the R3.1 *F annotations. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ *F Date: Tue, 9 Mar 2004 14:20:00 \-0500 (EST) *F From: Madeline Crosby *F Subject: Re: EP2509 is an allele of EcR *F To: flybase-help@morgan.harvard.edu, levis@ciwemb.edu *F Cc: crosby@morgan.harvard.edu *F Gillian, et al., *F CG8347 should be a synonym of EcR. It represents the 5' end *F of several EcR isoforms. *F \--Lynn # *U FBrf0178818 *a Escudero *b L.M. *t 2004.3.11 *T personal communication to FlyBase *u *F Date: Mon, 23 Feb 2004 09:30:55 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-10067 *F To: lmescudero@cbm.uam.es *F Cc: rd120@gen.cam.ac.uk *F Dear Luis, *F We are currently curating the abstracts for the upcoming 45th *F (Washington DC) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F charlatan, a gene encoding a Zn-finger factor involved in nervous system *F development. *F You mention a mutation that is new to FlyBase, chnEPIL#6, and say *F 'inserted near the transcription origin of chn' which indicates you *F know which CG chn corresponds to. If so, then please could you let me *F know which it is? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. chn would of course be the *F valid symbol for the locus after the chn record was merged with the CG record. *F Also, it sounds liek the EP insert is not one of the Gene Disruption *F Project inserts, but is novel. If you could confirm this for me that *F would be great. *F Thanks for your help. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Thu, 11 Mar 2004 10:46:04 \+0100 *F From: LuisMa *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-10067 *F Dear Rachel *F I am sorry for the delay *F Please find enclosed the information that you request. *F We consider that chn is CG11798. *F EPIL6 is a novel insertion of a EP element, it is located 122 pb *F 5' to the CG11798 predicted gene. *F With best wishes. *F Luis M. Escudero Cuadrado. # *U FBrf0178819 *a Thomas *b G. *t 2004.3.16 *T personal communication to FlyBase *u *F Date: Tue, 16 Mar 2004 23:34:50 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: Gene data (problem or question) *F comments: I was just looking at the entry for karst (cg12008) and noticed *F that kst 2-119 and 1-151 are listed as different alleles \- these are merely *F cleaned up chromosomes carrying kst 2 and kst 1 respectively. They should be *F eliminated as separate allele listing and perhaps included as synonyms. *F sorry for any confusion we created. with this designation. *F realname: Graham Thomas *F reply-to: gxt5@psu.edu *F source: FB-NG Help Mail: *F Sent from computer 146.186.153.134 # *U FBrf0178820 *a Kiger *b J. *t 2004.3.17 *T personal communication to FlyBase *u *F Date: Wed, 17 Mar 2004 14:46:01 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: John Kiger *F Subject: Re: Helping FlyBase: ADRC-10245 *F Dear Rachel, *F It turns out that the gene we just named as 'furled wing' is the *F bursicon gene identified in abstract 738C of McNabb et al. I have *F just communicated with Jim Truman and I agree with him that since *F bursicon is a long-established hormone, that should be its name. *F Regards, *F John *F \-- *F John A. Kiger, Jr. *F Professor of Genetics *F Section of Molecular & Cellular Biology *F University of California *F One Shields Avenue *F Davis, CA 95616 *F TEL 530-752-8204 *F FAX 530-752-3085 *F jakiger@ucdavis.edu # *U FBrf0178821 *a Levis *b R. *t 2004.3.9 *T personal communication to FlyBase *u *F Date: Tue, 9 Mar 2004 13:07:05 \-0500 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: lie gene may be EcR *F Cc: bazinetc@stjohns.edu *F The gene lie (long island expressway) is defined by a single mutant *F allele, the PZ insertion originally called ms(2)06410 (now called *F lie06410). FlyBase lists several synonyms for lie, such as *F ms(2)42A. When I BLAST the flanking insertion of ms(2)06410 (GenBank *F AQ026415), it maps to an intron of the EcR gene. Therefore, unless *F lie is a separate gene nested in an intron of EcR, it is likely that *F lie = EcR. This assumes that the P-element insertion is responsible *F for the sterile phenotype. I have not looked at the literature to *F see whether this has been verified. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0178822 *a Tran *b V. *c B. *d Edgar *t 2004.4.13 *T personal communication to FlyBase *u *F Date: Tue, 13 Apr 2004 16:45:06 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{tGPH} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Vuong Tran and Bruce Edgar, Fred Hutchinson Cancer Research *F Center (4/04). *F P{tGPH}2 is a homozygous viable and fertile, second chromosome *F insertion. P{tGPH}4 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178823 *a Bonini *b N. *t 2004.4 *T personal communication to FlyBase *u *F Date: Wed, 14 Apr 2004 09:53:18 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Bonini insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Nancy Bonini, University of Pennsylvania (4/04). *F P{UAS-Hsap\MJD.tr-Q78}c211.2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-Hsap\SNCA.F}5B, P{UAS-Hsap\SNCA.A30P}40.1, P{UAS-Hsap\SNCA.A53T}15.3 *F and P{UAS-Hsap\MJD.tr-Q27}N18.3d are homozygous viable and fertile, third *F chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178824 *a Murata *b T. *t 2004.4 *T personal communication to FlyBase *u *F Date: Wed, 14 Apr 2004 11:20:29 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: hrg constructs and insertions *F The following informaion accompanied stocks donated to the Bloomington *F Drosophila Stock Center by Takehide Murata, RIKEN Tsukuba Institute (4/04). *F P{UAS-hrg.DeltaHpaI-BamHI} is a construct deleting the region of the hrg *F gene containing the nuclear localization signal. It does not rescue the *F hrg wing phenotype when expressed with P{GawB}C-765. *F P{UAS-hrg.DeltaHpaI-BamHI}8-2-4 is a homozygous viable and fertile, second *F chromosome insertion. P{UAS-hrg.DeltaHpaI-BamHI}7-1-1 is a homozygous *F viable and fertile, third chromosome insertion. *F P{UAS-hrg.E}7-4 and P{UAS-hrg.D175A}46-3-2-2 are homozygous viable and *F fertile, third chromosome insertions. *F P{hrg+t6.8}11-1, P{UAS-hrg.E}7-3, P{UAS-hrg.D175A}46-1-2 and *F P{UAS-hrg.D175A}56-1-3 are second chromosome insertions. *F P{vg(D/V)-lacZ}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178825 *a Munoz *b E. *c K. *d Cook *t 2004.4.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Apr 2004 17:19:01 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: 'W3' confusion *F Cc: matthewk@indiana.edu, Enzo Muñoz *F Hi Rachel-- *F Enzo Munoz and I have been corresponding about a case of confusing *F synonymy. I thought I'd summarize what we have figured out. Perhaps you *F can archive this note in case someone tries to work on these genes and alleles. *F There are two mutant strains called 'W3'. One of the mutated X chromosomes *F carries mutations in the run and unc loci, and the other strain carries a *F mutation in shakB. The relevant allele entries are *F run9 FBal0014854 *F uncW3a FBal0095151 *F shakB15 FBal0015588 *F The W3 chromosome carrying run9 and uncW3a was described in Lifschytz *F and Yakobovitz (Molec. Gen. Genet. 1978 161:275--284 = FBrf0032163). They *F described recombination experiments that mapped lethality both to the left *F of l(1)19Ec (=l(1)J9) and shakB (=l(1)R-9-29) and to the right of l(1)19Ec. *F They also showed that the Dp(1;Y)mal+-linked lethal l(1)YT14 failed to *F complement W3. *F Lindsley and Zimm (DIS 68: 110, 1990) cite unpublished results of George *F Lefevre, Jr. showing that 'l(1)W3b' is an allele of shakB. In this *F reference l(1)W3b was called pas15. (Passover is a synonym for shaking *F B.) In Lindsley and Zimm, 1992 (the Redbook), the synonym for shakB15 *F is l(1)N36, but I suspect 'N36' was simply a case of 'W3b' being written *F down illegibly. *F Enzo received both W3 lines from Lifschytz and maintained them as distinct *F stocks. Crosses made by Abe Schalet and Enzo showed that the run9 *F uncW3a stock failed to complement run4 (=l(1)LB19), run2 (=l(1)AA33), *F run18 (=l(1)17-26), run17-44, run19 (=l(1)17-169) and Df(1)16-3-53 *F for lethality and Df(1)17-351 for the unc phenotype, yet complemented *F shakB6 (=l(1)E81), shakB11 (=l(1)R-9-29), shakB19 (=l(1)17-189) and *F l(1)19Ec8 (=l(1)R-9-28). *F Enzo worked less with the shakB15 stock, but nevertheless showed that it *F failed to complement *F shakB6 (=l(1)E81) and shakB11 (=l(1)R-9-29), yet complemented Df(1)17-351. *F The distinction between the two W3 stocks must have been clear to Lifschytz *F and Lefevre, because references to the run9 uncW3a chromosomes use *F 'W3a' in allele designations and references to the shakB15 chromosomes *F use 'W3b'. *F Nevertheless, the distinction was blurred in both Lindsley and Zimm 1990 *F and 1992, where l(1)YT14 was listed as an allele of shakB (=shakB17) *F (presumably because both l(1)YT14 and shakB alleles failed to complement *F something called 'W3'!). In Figure 2 of Lifschytz and Yakobovitz, 1978, *F l(1)YT14 is shown mapping to the left of shakB, a position consistent with *F it being an allele of run, rather than an allele of unc. This position of *F l(1)YT14 was probably determined from complementation tests with deletions *F to the left of shakB. *F I recommend the following changes to FlyBase entries: (1) The shakB17 *F allele entry should be eliminated and a new allele entry created for *F l(1)YT14 under the run gene--perhaps as runYT14. (The l(1)YT14 *F chromosome is extinct, so the purpose of this is to correct a historical *F mistake and prevent confusion.) (2) l(1)W3 and l(1)W3b should be listed *F as synonyms for shakB15. *F Thanks! *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178826 *a Finley *b K. *c R. *d Kraut *t 2004.4.16 *T personal communication to FlyBase *u *F Date: Fri, 16 Apr 2004 22:10:21 \-0700 *F From: Rachel Papan *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: beached/clue cheese *F Rachel, *F Thanks for letting me know about this. It's fine with me to rename it, *F so go ahead. *F Rachel. *F >------------- Begin Forwarded Message \------------- *F > *F >Date: Thu, 15 Apr 2004 10:21:24 \+0100 (BST) *F >From: 'Rachel Drysdale (Genetics)' *F >Subject: beached/clue cheese *F >To: zinnk@its.caltech.edu *F >Cc: rd120@gen.cam.ac.uk, finley@salk.edu *F > *F >Dear Kai, and Rachel, *F > *F >Kim Finley has been in touch with me about renaming FBgn0043362 *F >(currently Beach1: Beached 1) to bchs: blue cheese. Kim indicates that *F >this is acceptable to you. We do not generally rename genes in FlyBase *F >but while a gene record is new/small, and if (crucially) all groups *F >working on that gene agree to the change and intend to use the new name *F >in future publications then we can do it. So please confirm to me that *F >this is all in order. Our email correspondence will be archived as a *F >personal communication from you all to FlyBase, to act as the source *F >for this change. *F > *F >With best regards, *F > *F >Rachel. *F > *F >---------------------------------------------------------------------- *F >Rachel Drysdale, Ph.D. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: rd120@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk:7081/ *F >---------------------------------------------------------------------- # *U FBrf0178827 *a Greenspan *b R. *t 2004.4.19 *T personal communication to FlyBase *u *F Date: Mon, 19 Apr 2004 14:25:02 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-eag.Delta932}2 *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Ralph Greenspan, Neurosciences Institute (4/19). *F P{UAS-eag.Delta932}2 is a homozygous viable and fertile, second chromosome *F insertion. The construction and transformation of P{UAS-eag.Delta932} were *F described in Broughton et al., 2004, Current Biology 14: 538-547. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178828 *a Seroude *b L. *t 2004.4.29 *T personal communication to FlyBase *u *F Date: Thu, 29 Apr 2004 10:36:21 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Seroude insertions *F Hi Rachel-- *F I just got a shipment of P{GawB} insertions from Laurent Seroude. The *F lines he sent are listed below. All were described in Seroude et al., *F Aging Cell 1: 47--56, 2002 (=FBrf0160941) and a subset was described in *F Seroude, genesis 34: 34-38, 2002 (=FBrf0152373). *F Name:DJ626 *F Genotype: w1118,P{w+mW.hs=GawB}DJ626 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: decreases with age; nervous system, cardia, oenocytes *F Insertion: 94E-F, 5' of pointed (CG17077) *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ628 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: increases with age; few in nervous system, cardia, testis *F Insertion: 79F, 5' of CG11133 *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ631 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: constant with age; longitudinal muscles, cardia, testis *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ646 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: increases at mid-age; few sensory neurons, oenocytes, testis *F Insertion: between PGRP-LC (CG4432) and PGRP-LF (CG4437) *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ649 *F Chromosome: 1,2 *F Comments: insertion viable, fertile. *F Adult expression: constant with age *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ667 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: constant with age; muscles *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ684/CyO *F Chromosome: 1,2 *F Comments: lethal insertion *F Adult expression: decreases with age; nervous system *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ688/TM3,Sb *F Chromosome: 1,3 *F Comments: lethal insertion *F Adult expression: constant with age; brain, vertical muscles *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ690/CyO *F Chromosome: 1,2 *F Comments: lethal insertion *F Adult expression: decreases with age; brain, muscles *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ691 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: constant with age; longitudinal muscles, cardia, testis *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ694 *F Chromosome: 1,2 *F Comments: insertion viable, fertile. *F Adult expression: increases with age; muscles *F Insertion: 54B-C, In EDTP (CG6542). *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ695 *F Chromosome: 1,2 *F Comments: insertion viable, fertile. *F Adult expression: increases with age; nervous system *F Insertion: 54B, uridine kinase (CG4798) *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ703 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: small decrease with age; brain, muscles, retina *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ715 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: constant with age; brain, muscles, retina *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ717 *F Chromosome: 1,2 *F Comments: insertion semi-lethal *F Adult expression: decreases with age; nervous system, vertical muscles, crop *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ738 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: small decrease with age; *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ752 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: constant with age; few in the brain, muscles, cardia *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ755 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: small decrease with age; *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F +mW.hs=GawB}DJ757 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: constant with age; muscles *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ761 *F Chromosome: 1,3 *F Comments: insertion viable, fertile. *F Adult expression: increases with age; everywhere but nervous system *F Insertion: 85D, CG16765 *F References: Aging Cell, 1, 47-56, 2002; Genesis 34,34-38, 2002; *F +mW.hs=GawB}DJ847 *F Chromosome: 1,2 *F Comments: insertion viable, fertile. *F Adult expression: decreases with age *F Insertion: *F References: Aging Cell, 1, 47-56, 2002; *F *F Subject: Ollman insertions *F Cc: annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04) concerning insertions of constructs described in Ollman et al., 2000 *F (Cell 101: *F 91-101; FBrf0127259). *F P{UAS-p53.H159N.Ex}1 and P{GMR-p53.R155H.Ex}1 are homozygous and *F hemizygous, viable and fertile X chromosome insertions. *F P{GMR-p53.Ex}2 and P{UAS-p53.H159N.Ex}2 are homozygous viable and fertile, *F second chromosome insertions. *F P{UAS-p53.H159N.Ex}3 and P{GMR-p21.Ex}3 are homozygous viable and fertile, *F third chromosome insertions. *F P{GMR-p53.Ex}1 is an X chromosome insertion. *F P{UAS-p53.Ex}2, P{GMR-p53.R155H.Ex}2 and P{UAS-p53.R155H.Ex}2 are second *F chromosome insertions. *F P{GMR-p53.Ex}3, P{GMR-p53.R155H.Ex}3 and P{UAS-p53.Ex}3 are third *F chromosome insertions. *F P{GMR-p21.Ex}2 is an insertion on CyO. The Bloomington Stock Center will *F call this balancer variant CyO, P{w+mC=GMR-p21.Ex}2 in its stock list. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178830 *a Featherstone *b D. *t 2004.5.8 *T personal communication to FlyBase *u *F Date: Sat, 8 May 2004 09:49:06 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: annotation info *F comments: I notice the annotation for GOT1 is a little sparse. *F GOT1 is CG8430. We've cloned the cDNA and deposited that info in genbank, *F accession \# AY339650. *F GOT2, by the way, is CG4233. We haven't cloned that one yet. *F The E.C. identifier both GOTs is 2.6.1.1 *F realname: Dave Featherstone *F reply-to: def@uic.edu *F source: FB-NG Help Mail: *F usersubject: annotation info *F Sent from computer 24.13.77.138 # *U FBrf0178831 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u l(2)44DEa8. *F Date: Thu, 06 May 2004 13:28:03 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: l(2)44DEa8 *F Cc: flybase-updates@morgan.harvard.edu, annettep@sbcglobal.net *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F l(2)44DEa8 is an EMS-induced point mutation introducing a stop codon in *F CG8732. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178832 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u AP-47SAE-10 and AP-47SHE-11. *F Date: Thu, 06 May 2004 13:29:06 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: AP-47SAE-10 and AP-47SHE-11 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F AP-47SAE-10 and AP-47SHE-11 were both induced with EMS. AP-47SAE-10 *F is a S366N change. AP-47SHE-11 is a 37 nucleotide deletion resulting in *F the deletion of amino acids 146 to 158 and a frameshift. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178833 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u CG35111 and CG35112. *F Date: Thu, 06 May 2004 13:30:22 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: CG35111 and CG35112 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F CG35111 was induced with EMS. It consists of C578 to T and A579 to G *F followed by a 1 bp deletion of C581. This is predicted to cause a *F frameshift of N133->E and 24 novel amino acids before a stop. CG35112 is *F a gamma ray-induced allele of CG3511. It fails to complement *F CG35111. Transcript levels of CG3511 were reduced, but the molecular *F nature of the lesion was not defined. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178834 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u Nsf2A6 and Nsf2A15. *F Date: Thu, 06 May 2004 13:31:22 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Nsf2A6 and Nsf2A15 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F Nsf2A6 is an EMS-induced A597V change. Nsf2A15 is an EMS-induced 187 *F base pair deletion (1761 to 1948) and frameshift. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178835 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u Psn9, Psn143, Psn145 and Psn227. *F Date: Thu, 06 May 2004 13:32:18 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Psn9, Psn143, Psn145 and Psn227 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F Psn9 was induced with EMS and results in a L499Q change in the 541 amino *F acid Psn isoform. It appears to be hypomorphic. Psn143 is a deletion *F and/or splice site mutation resulting in deletion of the amino acids *F between the first transmembrane domain and the middle of the fourth *F transmembrane domain. It behaves as a null allele. Psn145 results in *F loss of amino acids 51 to 105. Psn227 results in a change of Q41 to stop. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178836 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u gammaTub23Cbmps1. *F Date: Thu, 06 May 2004 13:33:11 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: gammaTub23Cbmps1 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F gammaTub23Cbmps1 is an EMS-induced Met382 to Ile change. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178837 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u aph-1D35. *F Date: Thu, 06 May 2004 13:35:09 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: aph-1D35 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F l(2)23AB6D35 of Littleton and Bellen (FBal0039520) is a mutation in the *F anterior pharynx defective 1 gene (FBgn0031458). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178838 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u Rbfcas-21 and Rbfsls-15. *F Date: Thu, 06 May 2004 13:36:19 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Rbfcas-21 and Rbfsls-15 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F Rbfcas-21 is an EMS-induced mutation that makes a truncated protein of 102 *F aa. Rbfsls-15 is an 11 nucleotide X ray-induced deletion which results in *F the addition of 14 novel amino acids after amino acid 519 (total protein *F length of 533 aa). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178839 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u ebiCCS-6, ebiCCS-8 and ebiWKS-24. *F Date: Thu, 06 May 2004 13:34:08 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: ebiCCS-6, ebiCCS-8 and ebiWKS-24 *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F ebiCCS-6, ebiCCS-8 and ebiWKS-24 are mutations in the ebi gene. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178840 *a Parks *b A. *t 2004.5.6 *T personal communication to FlyBase *u Exelixis cloning vectors. *F Date: Thu, 06 May 2004 15:04:43 \-0500 *F To: rd120@gen.cam.ac.uk, crosby@morgan.harvard.edu *F From: Kevin Cook *F Subject: Exelixis cloning vectors *F Cc: flybase-updates@morgan.harvard.edu, Annette Parks *F , Kris Klueg *F The following information was provided by Annette Parks of Exelixis, Inc. *F (5/04). *F Exelixis constructed a variety of vectors which are all variations on *F P{Express}: *F P{Express-UAS} (FBtp0012992) *F P{Express-glass} (FBtp0012991) *F P{Express-vgMQ} *F P{Express-vgM} *F P{Express-ey3.5} *F P{Express-ey1x} *F P{Express-ey4x} *F P{Express-sev3x} *F P{Express-sev1x} *F P{Express-elav} *F P{Express-FRT.2.1} *F P{Express-FRT.2.2} *F 1. P{Express-vgMQ} *F Kim et al., 1996 (Nature 382:133-138; FBrf0088295) discusses the *F identification of two enhancers at the vestigial (FBgn0003975) gene. The *F 'quadrant' enhancer drives expression in the wing blade and the 'margin' *F enhancer drives expression at the dorsal/ventral boundary. Kim et al. *F combined the Q and M enhancers into a single contiguous fragment. This MQ *F element is what Exelixis cloned into pP{Express} to generate P{Express-vgMQ}. *F 2. P{Express-vgM} *F A similar construct similar to P{Express-vgMQ}, but carrying only the M *F enhancer. *F 3. P{Express-ey3.5} *F The DNA fragment driving expression in the pattern of the eyeless *F (FBgn0005558) gene is a 3.5 kb Kpn fragment described in Halder et al. 1998 *F (Development 125: 2181-2191; FBrf0103272). *F 4. P{Express-ey1x} *F Carries one copy of the 200 bp ey enhancer PCR amplified with Kpn/Bam ends. *F 5. P{Express-ey4x} *F Carries four copies of the eyeless enhancer. *F 6. P{Express-sev3x} and P{Express-sev1x} *F These two vectors carry either three copies or one copy of the sevenless *F (FBgn0003366) enhancer. The MacVector file gives the sequence of the *F P{Express-sev3x} enhancer. *F 7. P{Express-elav} *F Carries sequences from the elav (FBgn0000570) gene to drive neural expression. *F 8. P{Express-FRT.2.1} *F This vector was designed to allow a sequence to be cloned between FRT *F sites. The order of the sequences in the vector is *F FRT \- miniwhite \- Hsp70 TATA \- polylinker \- alphaTub84B 3'end \- FRT *F 9. P{Express-FRT.2.2} *F This vector was designed to allow the miniwhite marker to be removed after *F transformation. The order of the sequences in the vector is *F FRT \- miniwhite \- FRT \- Hsp70 TATA \- polylinker \- alphaTub84B 3'end *F 10. P{Express-FRT.2.3} *F This vector is identical to P{Express-FRT.2.2} except for the sequence of *F the polylinker, which has more restriction sites. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178841 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u aur constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: aur constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-aur.Exel}, the wild type aur (FBgn0000147) coding sequence is *F cloned into P{Express-UAS}. *F P{UAS-aur.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-aur.Exel}3 is a third chromosome insertion. *F In P{GMR-aur.Exel}, the wild type aur coding sequence is cloned into *F P{Express-glass}. *F P{GMR-aur.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178842 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u GAL4 constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: GAL4 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{ey3.5-GAL4.Exel}, the GAL4 gene was cloned into P{Express-ey3.5}. *F P{ey3.5-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{ey3.5-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{ey3.5-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{vgM-GAL4.Exel}, the GAL4 gene was cloned into P{Express-vgM}. *F P{vgM-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{vgM-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{ey1x-GAL4.Exel}, the GAL4 gene was cloned into P{Express-ey1x}. *F P{ey1x-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{ey1x-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{vgMQ-GAL4.Exel}, the GAL4 gene was cloned into P{Express-vgMQ}. *F P{vgMQ-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{vgMQ-GAL4.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{vgMQ-GAL4.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F P{m4-GAL4.Exel} carries GAL4 driven by the E(spl) region transcript m4 *F (FBgn0002629) enhancer. The m4 enhancer was originally PCR amplified from *F a clone described in Singson et al., 1994 (Genes Dev. 8: 2058-2071; *F FBrf0076492) and cloned into KpnI and XhoI sites in the polylinker of *F pP{Express}. The enhancer fragment plus flanking pP{Express}sequence was *F cut out as a 0.5 kb SmaI-AscI fragment and cloned into the same sites in *F pP{Express-GAL4}. *F P{m4-GAL4.Exel}2 is a second chromosome insertion. *F P{E(spl)-GAL4.Exel} carries GAL4 driven by the E(spl) (FBgn0000591) *F enhancer. The enhancer fragment was PCR amplified from a clone described *F in Lecourtois & Schweisguth, 1995 (Genes Dev. 9: 2598-2608; FBrf0084115) *F and Kramatschek & Campos-Ortega, 1994 (Development 20: 815-26; FBrf0073637) *F and cloned into KpnI and XhoI sites in the pP{Express} polylinker. The *F enhancer fragment was cut out as a 1.1 kb SmaI-AscI fragment and cloned *F into pP{Express-GAL4}. *F P{E(spl)-GAL4.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{E(spl)-GAL4.Exel}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178843 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Akt1 constructs and insertions. *F Date: Wed, 12 May 2004 15:18:23 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Akt1 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-Akt1.Exel}, wild type sequences for the short, 60 kD form of Akt1 *F (GenBank accession X83510) as described in Andjelkovic et al., 1995 (J. *F Biol. Chem. 270: 4066-4075; FBrf0079853) were cloned into P{Express-UAS}. *F P{GMR-Akt1.Exel}consists of the same sequence cloned into P{Express-glass}. *F P{GMR-Akt1.T342D.S505D}consists of the same sequence with Thr342Asp and *F Ser505Asp mutations cloned into P{Express-glass}. The protein variant is *F constitutively active. *F P{UAS-Akt1.Exel}1, P{GMR-Akt1.T342D.S505D}1 and P{GMR-Akt1.Exel}1 are *F homozygous and hemizygous viable and fertile, X chromosome insertions. *F P{UAS-Akt1.Exel}2, P{GMR-Akt1.Exel}2 and P{GMR-Akt1.T342D.S505D}2 are *F homozygous viable and fertile, second chromosome insertions. *F P{GMR-Akt1.T342D.S505D}3 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178844 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u FLP constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: FLP constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-FLP.Exel}, the FLP recombinase gene was cloned into P{Express-UAS}. *F P{UAS-FLP.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-FLP.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-FLP.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{ey1x-FLP.Exel}, the FLP recombinase gene was cloned into P{Express-ey1x}. *F P{ey1x-FLP.Exel}2 is a second chromosome insertions. *F In P{ey3.5-FLP.Exel}, the FLP recombinase gene was cloned into *F P{Express-ey3.5}. *F P{ey3.5-FLP.Exel}2 is a second chromosome insertions. *F In P{ey4x-FLP.Exel}, FLP recombinase gene was cloned into P{Express-ey4x}. *F P{ey4x-FLP.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{ey4x-FLP.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178845 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u RPIE-6. *F Date: Wed, 12 May 2004 15:22:40 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: RPIE-6 *F The following information was provided by Annette Parks of Exelixis, Inc. *F RPIE-6 is an EMS-induced mutation in the Roughened (FBgn0004636) gene *F causing an L120F amino acid change. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178846 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Ras85D constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Ras85D constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{vgMQ-Ras85D.V12}, the V12 mutant version of Ras85D of Karim and Rubin *F (Development 125; 1-9, 1998; FBrf0100097) was cloned into P{Express-vgMQ}. *F P{vgMQ-Ras85D.V12}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F In P{vgM-Ras85D.V12}, the V12 mutant version of Ras85D of Karim and Rubin *F (Development 125; 1-9, 1998; FBrf0100097) was cloned into P{Express-vgM}. *F P{vgM-Ras85D.V12}2 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178847 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u ebi constructs and insertions. *F Date: Wed, 12 May 2004 15:19:39 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: ebi constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-ebi.Exel}, wild type ebi (FBgn0023444) coding sequences were *F cloned into P{Express-UAS}. *F P{UAS-ebi.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-ebi.Exel}2 is a second chromosome insertion. *F P{UAS-ebi.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-ebi.HA.Sma}, ebi coding sequence with HA tag sequence placed into *F a unique SmaI site was cloned into P{Express-UAS}. The protein variant has *F the HA tag (YPYDVPDYASL) approximately 100 amino acids from the N terminus *F to give the internal sequence VKTEPGK-HAtag-P. *F P{UAS-ebi.HA.Sma}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-ebi.HA.Sma}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-ebi.HA.Nco}, ebi coding sequence with HA tag sequence placed into *F a unique NcoI site was cloned into P{Express-UAS}. The protein variant has *F the HA tag (YPYDVPDYASL) approximately 17 amino acids N terminal of the WD1 *F repeat to give the internal sequence VPM-HAtag-M (the M is duplicated). *F P{UAS-ebi.HA.Nco}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-ebi.HA.Nco}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-ebi.DN}, a truncated ebi sequence was cloned into *F P{Express-UAS}. The construct encodes a dominant negative protein variant *F that is essentially only the WD repeats at the C terminus. The ebi *F sequence starts GCCGCGCCCCGTCATGACTCCCGCAGCTCTTGTTCCCATGGACA. The initial *F GCCGCGCC is an artificial AcsI site. This is followed by a start *F ATG. This is then followed by the truncated ebi. *F P{UAS-ebi.DN}3 is a homozygous viable and fertile, third chromosome insertion. *F In P{ebi.FRT}, ebi coding sequences and upstream regulatory regions were *F cloned into P{Express-FRT.2.1}. Upon exposure to FLP recombinase, *F miniwhite and the ebi gene are excised from the construct. *F P{ebi.FRT}1 is a homozygous and hemizygous viable and fertile, X chromosome *F insertion. *F P{ebi.FRT}2 is a homozygous viable and fertile, second chromosome insertion. *F P{ebi.FRT}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178848 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u SCAP constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: SCAP constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-SCAP.Exel}, wild type SCAP (FBgn0033052) was cloned into *F P{Express-UAS}. A genomic fragment was used for the C-terminal half of *F SCAP; consequently, introns are still present. *F P{UAS-SCAP.Exel}3 is a third chromosome insertion. *F In P{UAS-SCAP.Y319C}, SCAP sequences encoding a constitutively active Y319C *F variant protein were cloned into P{Express-UAS}. A genomic fragment was *F used for the C-terminal half of SCAP; consequently, introns are still *F present. Y319C in flies mimics hamster Y298C as described in Nohturfft et *F al. (PNAS 95: 12848, 1998). *F P{UAS-SCAP.Y319C}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-SCAP.Y319C}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-SCAP.Y319C}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-SCAP.P450}, SCAP sequences encoding a dominant negative protein *F variant were cloned into P{Express-UAS}. The rabbit cytochrome P450 2c1 *F transmembrane domain (MDPVVVLGLCLSCLLLLSLWKQSYGGGKL) was appended to the C *F terminus just downstream of the final transmembrane domain to mimic a *F hamster construct described by Sakai et al. (J. Biol. Chem. 10: 5785, *F 1998). This modification results in permanent anchoring of SCAP protein to *F the endoplasmic reticulum. *F P{UAS-SCAP.P450}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-SCAP.P450}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178849 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u HLH106 constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: HLH106 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-HLH106.Exel}, wild type HLH106 (FBgn0015234) coding sequence was *F cloned into P{Express-UAS}. *F P{UAS-HLH106.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-HLH106.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-HLH106.Ndel}, HLH106 sequences encoding a dominant negative *F protein variant deleted for the highly acidic region at the N terminus *F (amino acids 1-75) were cloned into P{Express-UAS}. This construct mimics a *F construct of Sato et al. (J. Biol. Chem. 269: 17267, 1994). *F P{UAS-HLH106.Ndel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-HLH106.Ndel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-HLH106.Ndel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-HLH106.NTdel}, HLH106 sequences were cloned into P{Express-UAS} *F which encode a dominant negative protein variant deleted for the highly *F acidic region at the N terminus (amino acids 1-75) and missing the *F transmembrane domains. *F P{UAS-HLH106.NTdel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-HLH106.NTdel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-HLH106.Cdel}, HLH106 sequences encoding a constitutively active *F protein variant truncated right before the first transmembrane domain were *F cloned into P{Express-UAS}. The C terminal sequence is RGMATNSR. The *F variant protein is localized to the nucleus constitutively and is *F constitutively active. *F P{UAS-HLH106.Cdel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-HLH106.Cdel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{UAS-HLH106.P450}, HLH106 sequences were cloned into P{Express-UAS} *F which encode a dominant negative protein variant with the rabbit cytochrome *F P450 2c1 transmembrane domain (MDPVVVLGLCLSCLLLLSLWKQSYGGGKL) appended to *F the C-terminus. This modification results in permanent anchoring of HLH106 *F protein to the endoplasmic reticulum. *F P{UAS-HLH106.P450}1 is an X chromosome insertion. *F P{UAS-HLH106.P450}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-HLH106.P450}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178850 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Nmdar1 constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Nmdar1 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-Nmdar1.Exel}, wild type Nmdar1 (FBgn0010399) coding sequences were *F cloned into P{Express-UAS}. May be missing amino acids 571-573, 833-855 *F and 965-969. *F P{UAS-Nmdar1.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Nmdar1.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{GMR-Nmdar1.Exel},wild type Nmdar1 coding sequences were cloned into *F P{Express-glass}. May be missing amino acids 571-573, 833-855 and 965-969. *F P{GMR-Nmdar1.Exel}1 is an X chromosome insertion. *F P{GMR-Nmdar1.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-Nmdar1.lurcher}, Nmdar1 sequences carrying the mouse lurcher *F mutation were cloned into P{Express-UAS}. The lurcher mutation changes a *F conserved YTANLAAF to YTANLATF. *F P{UAS-Nmdar1.lurcher}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-Nmdar1.lurcher}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-Nmdar1.lurcher}, Nmdar1 sequences carrying the mouse lurcher *F mutation were cloned into P{Express-glass}. The lurcher mutation changes a *F conserved YTANLAAF to YTANLATF. *F P{GMR-Nmdar1.lurcher}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-Nmdar1.lurcher}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{GMR-Nmdar1.lurcher}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178851 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Spt5MGE-3. *F Date: Wed, 12 May 2004 15:22:40 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Spt5MGE-3 *F The following information was provided by Annette Parks of Exelixis, Inc. *F Spt5MGE-3 is an EMS-induced mutation in the Spt5 (FBgn0040273) gene *F causing a K236stop amino acid change. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178852 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u rin construct and insertion. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: rin construct and insertion *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{GMR-rin.Exel}, the wild type rin (FBgn0015778) coding sequence from *F cDNA LD15503 was cloned into P{Express-glass}. *F P{GMR-rin.Exel}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178853 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Glu-RI constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Glu-RI constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-Glu-RI.lurcher}, Glu-RI (FBgn0004619) sequence encoding a variant *F protein was cloned into P{Express-UAS}. The sequence carries the mouse *F lurcher mutation, which changes a conserved YTANLAAF to YTANLATF. Amino *F acids 34-42, 359-382, 461-470, 640-662 and 896-916 may be missing relative *F to the sequence given in accession AAA28575. *F P{UAS-Glu-RI.lurcher}2 is a homozygous viable and fertile, second *F chromosome insertion. *F In P{GMR-Glu-RI.lurcher}, Glu-RI sequence encoding a variant protein was *F cloned into P{Express-glass}. The sequence carries the mouse lurcher *F mutation which changes a conserved YTANLAAF to YTANLATF. Amino acids *F 34-42, 359-382, 461-470, 640-662 and 896-916 may be missing relative to the *F sequence given in accession AAA28575. *F P{GMR-Glu-RI.lurcher}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-Glu-RI.lurcher}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{GMR-Glu-RI.lurcher}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178854 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Flip-out piggyBac transposase constructs and insertions. *F Date: Wed, 12 May 2004 15:25:50 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Flip-out piggyBac transposase constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{FRT(w+)Tub-PBac\T}, the piggyBac transposase coding region with the *F 3' UTR from fs(1)K10 under the control of the alphaTub84B promoter was *F cloned into P{Express-FRT.2.2}. Upon exposure to FLP recombinase, the *F miniwhite gene positioned between FRT sites is excised. The rearranged *F construct is P{Tub-PBac\T}. Both P{FRT(w+)Tub-PBac\T} and P{Tub-PBac\T} *F express piggyBac transposase. *F P{FRT(w+)Tub-PBac\T}2 is an insertion on CyO. P{Tub-PBac\T}2 is the same *F insertion after excision of the miniwhite cassette by FLP recombinase. *F In P{FRT(w+)Hsp70-PBac\T}, the piggyBac transposase coding region under *F the control of the Hsp70 promoter was cloned into P{Express-FRT.2.3}. Upon *F exposure to FLP recombinase, the miniwhite gene positioned between FRT *F sites is excised. The rearranged construct is P{Hsp70-PBac\T}. Both *F P{FRT(w+)Hsp70-PBac\T} and P{Hsp70-PBac\T} express piggyBac transposase *F upon heat shock. *F P{FRT(w+)Hsp70-PBac\T}2 is an insertion on CyO. P{Hsp70-PBac\T}2 is the *F same insertion after excision of the miniwhite cassette by FLP recombinase. *F The sequence of the alphaTub84B-PBac\T-fs(1)K10 3' UTR insert is given *F below and in attached files. *F GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT *F GAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT *F ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATC *F AGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACG *F TTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC *F GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGA *F CAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGG *F ACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCG *F TTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG *F CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTG *F CAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCG *F CGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT *F ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACT *F CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCAT *F GACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGAT *F CCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAG *F AGCTACCAACTCTTTTT *F CCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCA *F AGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTG *F TCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAG *F CCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAG *F GGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGC *F CTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGG *F AGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTC *F CTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGAC *F CGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATT *F CATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATG *F TGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGG *F ATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAG *F CTGGAGCTCCACCGCGGTGGCGGCCGCTTCGAGTCCCAACTAGTCCTGCAAAATACCGCAGCAAGTTTTAGAGAGACCA *F AGTGCCATTACCTCTCCCACTTCAGTTATCGGTTATGCGGCGTTTAAGTCGACAGCTTGCCGTCTCTAGCTCCGGTGCC *F TATATAAAGCAGCCCGCTTTCCACATTTCATATTCGTTTTACGTTTGTCAAGCCTCATAGCCGGCAGTTCGAACGTATA *F CGCTCTCTGAGTCAGACCTCGAAATCGTAGCTCTACACAATTCTGTGAATTTTCCTTGTCGCGTGTGAAACACTTCCAA *F TAAAAACTCAATATGGTGAGTACTTTAAAAAAAAATCTAGTGAAATAATGCTGAAAAGAAATTTGTGTGGGCAAAATTC *F AATGGGCAAAAACGCGATGCGGCTTTTTCTCAAAATGGCGGCCGGCCTGCGTTTTTTCCTCAAAAGTGATGACGTCATG *F CCTGTTTTTTTTTTTTGTTCGCAATGAGGAATGGCTCTTAAAATCTAGATAAAAAAAATATTCATTATTTCTATGCTGC *F TGGAACGCTTCATTAATCTTAAAAATTCTAAATTCGGTTACCATGATACTTCGACGCATAACTGTAGATTTTGGATAGA *F ATTAAAGAGAAAATGGCGAGAGAG *F TAAAATTCCGGCGTCGGCAAAGTAGAGCAAAAAAATCAGTATACCATTTAGCTACCTCTCTCACTCGCACGCAGTGCCG *F GCTCAAGTTGGGCGCGGCTCTGCAATTATCGATTTTCTTGGGGTGTGTAACTAATCATCCGTTTTCCCCTCCTCCTCAT *F CCACAGCGTGGGATCCTGGGTAGTTCTTTAGACGATGAGCATATCCTCTCTGCTCTTCTGCAAAGCGATGACGAGCTTG *F TTGGTGAGGATTCTGACAGTGAAATATCAGATCACGTAAGTGAAGATGACGTCCAGAGCGATACAGAAGAAGCGTTTAT *F AGATGAGGTACATGAAGTGCAGCCAACGTCAAGCGGTAGTGAAATATTAGACGAACAAAATGTTATTGAACAACCAGGT *F TCTTCATTGGCTTCTAACAGAATCTTGACCTTGCCACAGAGGACTATTAGAGGTAAGAATAAACATTGTTGGTCAACTT *F CAAAGTCCACGAGGCGTAGCCGAGTCTCTGCACTGAACATTGTCAGATCTCAAAGAGGTCCGACGCGTATGTGCCGCAA *F TATATATGACCCACTTTTATGCTTCAAACTATTTTTTACTGATGAGATAATTTCGGAAATTGTAAAATGGACAAATGCT *F GAGATATCATTGAAACGTCGGGAATCTATGACAGGTGCTACATTTCGTGACACGAATGAAGATGAAATCTATGCTTTCT *F TTGGTATTCTGGTAATGACAGCAGTGAGAAAAGATAACCACATGTCCACAGATGACCTCTTTGATCGA *F TCTTTGTCAATGGTGTACGTCTCTGTAATGAGTCGTGATCGTTTTGATTTTTTGATACGATGTCTTAGAATGGATGACA *F AAAGTATACGGCCCACACTTCGAGAAAACGATGTATTTACTCCTGTTAGAAAAATATGGGATCTCTTTATCCATCAGTG *F CATACAAAATTACACTCCAGGGGCTCATTTGACCATAGATGAACAGTTACTTGGTTTTAGAGGACGGTGTCCGTTTAGG *F ATGTATATCCCAAACAAGCCAAGTAAGTATGGAATAAAAATCCTCATGATGTGTGACAGTGGTACGAAGTATATGATAA *F ATGGAATGCCTTATTTGGGAAGAGGAACACAGACCAACGGAGTACCACTCGGTGAATACTACGTGAAGGAGTTATCAAA *F GCCTGTGCACGGTAGTTGTCGTAATATTACGTGTGACAATTGGTTCACCTCAATCCCTTTGGCAAAAAACTTACTACAA *F GAACCGTATAAGTTAACCATTGTGGGAACCGTGCGATCAAACAAACGCGAGATACCGGAAGTACTGAAAAACAGTCGCT *F CCAGGCCAGTGGGAACATCGATGTTTTGTTTTGACGGACCCCTTACTCTCGTCTCATATAAACCGAAGCCAGCTAAGAT *F GGTATACTTATTATCATCTT *F GTGATGAGGATGCTTCTATCAACGAAAGTACCGGTAAACCGCAAATGGTTATGTATTATAATCAAACTAAAGGCGGAGT *F GGACACGCTAGACCAAATGTGTTCTGTGATGACCTGCAGTAGGAAGACGAATAGGTGGCCTATGGCATTATTGTACGGA *F ATGATAAACATTGCCTGCATAAATTCTTTTATTATATACAGCCATAATGTCAGTAGCAAGGGAGAAAAGGTCCAAAGTC *F GCAAAAAATTTATGAGAAACCTTTACATGAGCCTGACGTCATCGTTTATGCGTAAGCGTTTAGAAGCTCCTACTTTGAA *F GAGATATTTGCGCGATAATATCTCTAATATTTTGCCAAATGAAGTGCCTGGTACATCAGATGACAGTACTGAAGAGCCA *F GTAATGAAAAAACGTACTTACTGTACTTACTGCCCCTCTAAAATAAGGCGAAAGGCAAATGCATCGTGCAAAAAATGCA *F AAAAAGTTATTTGTCGAGAGCATAATATTGATATGTGCCAAAGTTGTTTCTGACTGACTAATAAGTATAATTTGTTTCT *F ATTATGTATAAGTTAAGCTAATTACTTATTTTATAATACAACATGACTGTTTTTAAAGTACAAAA *F TAAGTTTATTTTTGTAAAAGAGAGAATGTTTAAAAGTTTTGTTACTTTATAGAAGAAATTTTGAGTTTTTGTTTTTTTT *F TAATAAATAAATAAACATAAATAAATTGTTTGTTGAATTTATTATTAGTATGTAAGTGTAAATATAATAAAACTTAATA *F TCTATTCAAATTAATAAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAATTTTACGCATGATTATCTTTAA *F CGTACGAGGCCTTAGATTACACCACTTGATTGTATTTTTAAATTAATTCTTAAAAACTACAAATTAAGATCACTCTGTG *F AACGTGTGCTCGATGGTGTGCATCTACGGATTTGTTTTTGTGTTCTTTTTCCCCACCCAACCCCGTCCCTGACCCAATT *F CCGCTGTTCTCTTTACCGACTTGTAGATTAATCACGCTCTTGATCTTCATGACCGGATCCACCTGACAAGTAACGCCAA *F AGCAGCCCCACTTAG *F CGGAATAGTAGAGCCTAGGGGACCCAACGTAAAAACTCTAGTATAGCACCAATCTCCCAACCAGTTAACATTATACCTA *F AACCCATGGTCAAGAGTAAACATTTCTGCCTTTGAAGTTGAGAACACAATTAAGCATCCCCTGGTTAAACCTGACATTC *F ATACTTGTTAATAGCGCCATAAACATAGCACCAATTTCGAAGAAATCAGTTAAAAGCAATTAGCAATTAGCAATTAGCA *F ATAACTCTGCTGACTTCAAAACGAGAAGAGTTACAAGTATTTGTAAGGCACAGTTTATAGACCACCGACGGCTCGTTAG *F GGCTCGTCATGTAACTAAGCGCGGTGAAACCCAATTGAACATATAGTGGAATTATTATTATCAATGGGGAATATTTAAC *F CCTCAGGTAGCAAAGTAATTTAATTGCAAATAGAGAGTCCTAAGACTAAATAATATATTTAAAAATCTGGCCCTTTGAC *F CTTGCTTGTCAGGTGCATTTGGGTTCAATCGTAAGTTGCTTCTATATAAACACTTTCCCCATCCCCGCAATAATGAAGA *F ATACCGCAGAATAAAGAGAGATTTGCAACAAAAAATAAAGGCATTGCGAAAACTTTTTATGGGGGATCATTACACTCGG *F GCCTACGGTTACAATTCCCAGCCACTTAAGCGACAAGTTTGGCCAACAATCCATCTAATAGCTAATAGCGCAATCACTG *F GTAATCGCAAGAGTATATAGGCAATAGAACCCATGGATTTGCCCAAAGGTAACCGAGACAATGGAGAAGCAAGAGGATT *F TCAAACTGAACACCCACAGTGCTGTGTACTACCACTGGCGCGTTTGGGAGCTCACTGG *F CCTGATGCGTCCTCCGGGCGTTTCAAGCCTGCTTTACGTGGTATACTCCATTACGGTCAACTTGGTGGTCACCGTGCTG *F TTTCCCTTGAGCTTGCTGGCCAGGCTGCTGTTCACCACCAACATGGCCGGATTGTGCGAGAACCTGACCATAACTATTA *F CCGATATTGTGGCCAATTTGAAGTTTGCGAATGTGTACATGGTGAGGAAGCAGCTCCATGAGATTCGCTCTCTCCTAAG *F GCTCATGGACGCTAGAGCCCGGCTGGTGGGCGATCCCGAGGAGATTTCTGCCTTGAGGAAGGAAGTGAATATCGCACAG *F GGCACTTTCCGCACCTTTGCCAGTATTTTCGTATTTGGCACTACTTTGAGTTGCGTCCGCGTGGTCGTTCGCCCGGATC *F GAGAGCTCCTGTATCCGGCCTGGTTCGGCGTTGACTGGATGCACTCCACCAGAAACTATGTGCTCATCAATATCTACCA *F GCTCTTCGGCTTGATAGTGCAGGCTATACAGAACTGCGCTAGTGACTCCTATCCGCCTGCGTTTCTCTGCCTGCTCACG *F GGTCATATGCGTGCTTTGGAGCTGAGGGTGCGGCGGATTGGCTGCAGGACGGAAAAGTCCAATAAAGGGC *F AGACGGTACCAAGCTTGATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATT *F ACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGC *F ACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAAT *F GGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTG *F CCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCT *F AAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGT *F TCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCT *F TGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTA *F TTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAG *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178855 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u polo constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: polo constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-polo.T182D}, the polo (FBgn0003124) coding sequence with a T182D *F change was cloned into P{Express-UAS}. The encoded mutant polo protein *F should be constitutively active and equivalent to the human mutant T210D *F protein. *F P{UAS-polo.T182D}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-polo.T182D}, the polo coding sequence with a T182D change was *F cloned into P{Express-glass}. *F P{GMR-polo.T182D}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178856 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u InR constructs and insertions. *F Date: Wed, 12 May 2004 15:19:39 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: InR constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{GMR-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to *F accession AAC47458 were cloned into P{Express-glass}. *F P{GMR-InR.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-InR.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-InR.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to *F accession AAC47458 were cloned into P{Express-UAS}. *F P{UAS-InR.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{sev-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to *F accession AAC47458 were cloned into either P{Express-sev1x} or *F P{Express-sev3x}. *F P{sev-InR.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{sev-InR.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{sev-InR.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-InR.del}, InR sequence deleted from Eco47III to XhoI to delete *F most of the alpha subunit was cloned into P{Express-UAS}. This construct *F mimics a construct expressing a constitutively active human InR described *F by Ellis et al., 1987 (Mol Endocrin. 1:15-24). *F P{UAS-InR.del}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-InR.del}3 is a homozygous viable and fertile, third chromosome insertion. *F In P{GMR-InR.del}, InR sequence deleted from Eco47III to XhoI to delete *F most of the alpha subunit was cloned into P{Express-glass}. This construct *F mimics a construct expressing a constitutively active human InR described *F by Ellis et al., 1987 (Mol Endocrin. 1:15-24). *F P{GMR-InR.del}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-InR.del}3 is a homozygous viable and fertile, third chromosome insertion. *F In P{UAS-InR.R418P}, InR sequences encoding a constitutively active R418P *F variant protein were cloned into P{Express-UAS}. This fly variant protein *F mimics the human K86P protein variant of Longo et al., 1993 (PNAS 90: 60-64). *F P{UAS-InR.R418P}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-InR.R418P}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{GMR-InR.R418P}, InR sequences encoding a constitutively active R418P *F variant protein were cloned into P{Express-glass}. This fly variant *F protein mimics the human K86P protein variant of Longo et al., 1993 (PNAS *F 90: 60-64). *F P{GMR-InR.R418P}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-InR.R418P}3 is a third chromosome insertion. *F In P{UAS-InR.K1409A}, InR sequences encoding a dominant negative K1409A *F protein variant were cloned into P{Express-UAS}. *F P{UAS-InR.K1409A}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-InR.K1409A}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-InR.K1409A}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-InR.K1409A}, InR sequences encoding a dominant negative K1409A *F protein variant were cloned into P{Express-glass}. *F P{GMR-InR.K1409A}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-InR.K1409A}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-InR.K1409A}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-InR.A1325D}, InR sequences encoding a constitutively active A1325D *F variant protein were cloned into P{Express-UAS}. This fly variant protein *F mimics the human V938D protein variant of Longo et al., 1992 (J Biol. Chem. *F 267:12416-9). *F P{UAS-InR.A1325D}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-InR.A1325D}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-InR.A1325D}, InR sequences encoding a constitutively active A1325D *F variant protein were cloned into P{Express-glass}. This fly variant *F protein mimics the human V938D protein variant of Longo et al., 1992 (J *F Biol. Chem. 267:12416-9). *F P{GMR-InR.A1325D}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-InR.A1325D}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178857 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u RhoGAPp190 construct and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: RhoGAPp190 construct and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{GMR-RhoGAPp190.Exel}, wild type RhoGAPp190 sequences from cDNA GH02343 *F were cloned into P{Express-glass}. *F P{GMR-RhoGAPp190.Exel}1 is a homozygous and hemizygous viable and fertile, *F X chromosome insertion. *F P{GMR-RhoGAPp190.Exel}2 is a homozygous viable and fertile, second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178858 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Pi3K92E constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Pi3K92E constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-Pi3K92E.Exel}, the coding region of wild type Pi3K92E *F (FBgn0015279) was cloned into P{Express-UAS}. *F P{UAS-Pi3K92E.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Pi3K92E.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-Pi3K92E.Exel}, the coding region of wild type Pi3K92E was cloned *F into P{Express-GMR}. *F P{GMR-Pi3K92E.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{UAS-Pi3K92E.A2860C}, Pi3K92E sequences with a A2860C nucleotide change *F were cloned into P{Express-UAS}. The A2860C mutation changes an Asp to Ala *F in the ATP binding site and results in a dominant negative allele. *F P{UAS-Pi3K92E.A2860C}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Pi3K92E.A2860C}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-Pi3K92E.A2860C}, Pi3K92E sequences with a A2860C nucleotide change *F were cloned into P{Express-glass}. The A2860 mutation changes an Asp to *F Ala in the ATP binding site and results in a dominant negative allele. *F P{GMR-Pi3K92E.A2860C}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{GMR-Pi3K92E.A2860C}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F In P{GMR-Pi3K92E.CAAX}, Pi3K92E coding sequence with a farnesylation signal *F (CAAX) added to the 3' end were cloned into P{Express-glass}. The encoded *F protein is constitutively active. *F P{GMR-Pi3K92E.CAAX}2 is a second chromosome insertion. *F In P{UAS-Pi3K92E.CAAX}, Pi3K92E coding sequence with a farnesylation signal *F (CAAX) added to the 3' end were cloned into P{Express-UAS}. The encoded *F protein is constitutively active. *F P{UAS-Pi3K92E.CAAX}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178859 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u rho construct and insertion. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: rho construct and insertion *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{GMR-rho.Exel}, the wild type rho (FBgn0004635) coding sequence was *F cloned into P{Express-glass}. *F P{GMR-rho.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178860 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u scu constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: scu constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-scu.Exel}, the wild type scu (FBgn0021765) coding region was *F cloned into the BglII and Not sites of P{Express-UAS}. *F P{UAS-scu.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-scu.Exel}2 is a second chromosome insertion. *F In P{GMR-scu.Exel}, the scu (FBgn0021765) coding region was cloned into the *F BglII and Not sites of P{Express-glass}. *F P{GMR-scu.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178861 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Psn constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Psn constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-Psn.541.Exel}, the sequence encoding the 541 amino acid isoform of *F Psn (FBgn0019947) was cloned into P{Express-UAS). *F P{UAS-Psn.541.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Psn.541.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Psn.541.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-Psn.541.N157I.Exel}, Psn sequences encoding a N157I variant of the *F 541 amino acid isoform were cloned into P{Express-UAS). The N157I change *F in flies mimics the N141I change in humans. *F P{UAS-Psn.541.N157I.Exel}1 is a homozygous and hemizygous viable and *F fertile, X chromosome insertion. *F P{UAS-Psn.541.N157I.Exel}3 is a homozygous viable and fertile, third *F chromosome insertion. *F In P{UAS-Psn.541.M157V}, Psn sequences encoding a M157V variant of the 541 *F amino acid isoform were cloned into P{Express-UAS). The M157V change in *F flies mimics the M239V change in humans. *F P{UAS-Psn.541.M157V}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Psn.541.M157V}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Psn.541.M157V}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-antisense.Psn.541}, the sequence encoding the 541 amino acid *F isoform of Psn was cloned into P{Express-UAS) in an antisense direction. *F P{UAS-antisense.Psn.541}1 is a homozygous and hemizygous viable and *F fertile, X chromosome insertion. *F P{UAS-antisense.Psn.541}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-antisense.Psn.541}3 is a third chromosome insertion. *F In P{UAS-Psn.527.Exel}, the sequence encoding the 527 amino acid isoform of *F Psn was cloned into P{Express-UAS). *F P{UAS-Psn.527.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Psn.527.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Psn.527.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{sev-Psn.527.Exel}, the sequence encoding the 527 amino acid isoform of *F Psn was cloned into either P{Express-sev1x) or P{Express-sev3x}. *F P{sev-Psn.527.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{sev-Psn.527.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-Psn.527.Exel}, the sequence encoding the 527 amino acid isoform of *F Psn was cloned into P{Express-glass). *F P{GMR-Psn.527.Exel}2 is a second chromosome insertion. *F P{GMR-Psn.527.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-Psn.527.D447A}, Psn sequences encoding a D447A variant of the 527 *F amino acid isoform were cloned into P{Express-UAS). The D447A change in *F flies mimics the D385A change in humans. *F P{UAS-Psn.527.D447A}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Psn.527.D447A}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Psn.527.D447A}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-Psn.527.D447A}, Psn sequences encoding a D447A variant of the 527 *F amino acid isoform were cloned into P{Express-glass). The D447A change in *F flies mimics the D385A change in humans. *F P{GMR-Psn.527.D447A}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-Psn.527.D447A}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{GMR-Psn.527.D447A}3 is a homozygous viable and fertile, third *F chromosome insertion. *F In P{UAS-Psn.527.deltaE9}, Psn sequences encoding a variant of the 527 *F amino acid isoform with the equivalent of human exon 9 deleted and replaced *F with a cys were cloned into P{Express-UAS}, i.e. nucleotides *F 1078-1170 *F (TCCACTGTCGTTTACGCACTTGTAAACACTGTTACGCCGCAGCAATCGCAGGCCCAGCTTCCTCCTCGCCGTCGTCCA *F GCAACTCCACCACA) *F in AF017024 were replaced with a cys. *F P{UAS-Psn.527.deltaE9}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-Psn.527.deltaE9}3 is a homozygous viable and fertile, third *F chromosome insertion. *F In P{GMR-Psn.527.deltaE9}, Psn sequences encoding a variant of the 527 *F amino acid isoform with the equivalent of human exon 9 deleted and replaced *F with a cys were cloned into P{Express-glass}, i.e. nucleotides *F 1078-1170 *F (TCCACTGTCGTTTACGCACTTGTAAACACTGTTACGCCGCAGCAATCGCAGGCCCAGCTTCCTCCTCGCCGTCGTCCA *F GCAACTCCACCACA) *F in AF017024 were replaced with a cys. *F P{GMR-Psn.527.deltaE9}1 is a homozygous and hemizygous viable and fertile, *F X chromosome insertion. *F P{GMR-Psn.527.deltaE9}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{GMR-Psn.527.deltaE9}3 is a homozygous viable and fertile, third *F chromosome insertion. *F In P{UAS-Psn.527.deltaE9.D447A}, Psn sequences encoding a variant of the *F 527 amino acid isoform with two alterations cloned into *F P{Express-UAS}. First, the equivalent of human exon 9 was deleted and *F replaced with a cys, i.e. nucleotides *F 1078-1170 *F (TCCACTGTCGTTTACGCACTTGTAAACACTGTTACGCCGCAGCAATCGCAGGCCCAGCTTCCTCCTCGCCGTCGTCCA *F GCAACTCCACCACA) *F in AF017024 were replaced with a cys. Second, a D447A change was introduced *F to mimic the D385A change in humans. *F P{UAS-Psn.527.deltaE9.D447A}1 is a homozygous and hemizygous viable and *F fertile, X chromosome insertion. *F P{UAS-Psn.527.deltaE9.D447A}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-Psn.527.deltaE9.D447A}3 is a homozygous viable and fertile, third *F chromosome insertion. *F In P{GMR-Psn.527.deltaE9.D447A}, Psn sequences encoding a variant of the *F 527 amino acid isoform with two alterations were cloned into *F P{Express-glass}. First, the equivalent of human exon 9 was deleted and *F replaced with a cys, i.e. nucleotides *F 1078-1170 *F (TCCACTGTCGTTTACGCACTTGTAAACACTGTTACGCCGCAGCAATCGCAGGCCCAGCTTCCTCCTCGCCGTCGTCCA *F GCAACTCCACCACA) *F in AF017024 were replaced with a cys. Second, a D447A change was introduced *F to mimic the D385A change in humans. *F P{GMR-Psn.527.deltaE9.D447A}1 is a homozygous and hemizygous viable and *F fertile, X chromosome insertion. *F P{GMR-Psn.527.deltaE9.D447A}2 is a homozygous viable and fertile, second *F chromosome insertion. *F In P{GMR-Psn.527.D279A}, Psn sequences encoding a D279A variant of the 527 *F amino acid isoform were cloned into P{Express-glass). The D279A change in *F flies mimics the D257A change in humans. *F P{GMR-Psn.527.D279A}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-Psn.527.D279A}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178862 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u lacZ constructs and insertions. *F Date: Wed, 12 May 2004 15:23:46 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: lacZ constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{ey3.5-lacZ}, lacZ was cloned into EcoR1/Bgll2 sites in the polylinker *F of P{Express-ey3.5}. The lacZ gene was isolated in two fragments: *F EcoR1-Sac1 was from pC4-AUG-betagal (FBmc0000220) and is an in-frame fusion *F of the Adh leader to the 5' end of lacZ. The other half of lacZ was from *F pDCZA (a clone from Dan Curtis) and has the 3' end of lacZ directly fused *F to the Adh 3'UTR immediately following the stop codon. *F P{ey3.5-lacZ}2 is a homozygous viable and fertile, second chromosome insertion. *F P{ey3.5-lacZ}3 is a homozygous viable and fertile, third chromosome insertion. *F In P{vgM-lacZ.Exel}, lacZ was cloned into EcoR1/Bgll2 sites in the *F polylinker of P{Express-vgM}. The lacZ gene was isolated in two fragments: *F EcoR1-Sac1 was from pC4-AUG-betagal (FBmc0000220) and is an in-frame fusion *F of the ADH leader to the 5' end of lacZ. The other half of lacZ was from *F pDCZA (a clone from Dan Curtis) and has the 3' end of lacZ directly fused *F to the ADH 3'UTR immediately following the stop codon. *F P{vgM-lacZ.Exel}2 is a second chromosome insertion. *F P{vgM-lacZ.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{GMR-UAS-lacZ}, a UAS-Hsp70TATA-lacZ fragment was cloned into *F P{Express-glass} in place of the Hsp70 TATA sequence of that vector using *F KpnI and SacII. The result was GMR-UAS-Hsp70TATA-lacZ. Both UAS and GMR *F can drive lacZ expression. *F P{GMR-UAS-lacZ}2 is a second chromosome insertion. *F P{GMR-UAS-lacZ}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{Dll-UAS-lacZ}, a Distalless (FBgn0000157) enhancer was placed 5' of *F UAS-Hsp70TATA-lacZ in P{Express} using XhoI and KpnI. lacZ is expressed in *F the embryonic Dll pattern, but not in imaginal disks. UAS can also drive *F lacZ expression. *F P{Dll-UAS-lacZ}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F In P{dpp-lacZ.Exel.1} and P{dpp-lacZ.Exel.2}, the dpp disk enhancer *F described in St. Johnston et al., 1990 (Genes Dev. 4: 1114-1127; *F FBrf0051824) drives lacZ expression. In P{dpp-lacZ.Exel.1}, the relative *F orientation of the 3 kb enhancer fragment to lacZ matches the orientation *F of the enhancer and the dpp coding region in vivo and in P{TnJMB7} *F (FBtp0004773) described in Masucci et al., 1990 (Genes. Dev. 4: 2011-2023; *F FBrf0051842). In P{dpp-lacZ.Exel.2}, the enhancer is in the opposite *F orientation and matches that in P{TnJMB2} (FBtp0004772) also described in *F Masucci et al. *F P{dpp-lacZ.Exel.1}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{dpp-lacZ.Exel.2}1is an X chromosome insertion. *F P{dpp-lacZ.Exel.2}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{dpp-lacZ.Exel.2}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178863 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Bub1 constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Bub1 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-Bub1.Exel}, the wild type Bub1 (FBgn0025458) coding sequence was *F cloned into P{Express-UAS}. *F P{UAS-Bub1.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Bub1.Exel}2 is a second chromosome insertion. *F P{UAS-Bub1.Exel}3 is a third chromosome insertion. *F In P{UAS-Bub1.DN}, the Bub1 coding sequence truncated at the Stu1 site was *F cloned into P{Express-UAS}. The C terminal sequence of this dominant *F negative protein variant should be LEQPFRFPTNFVAKN. *F P{UAS-Bub1.DN}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-Bub1.DN}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Bub1.DN}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178864 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u arm constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: arm constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-arm.Exel}, wild type arm (FBgn0000117) was cloned into *F P{Express-UAS}. The arm sequence was PCR amplified using forward primer *F GCGCGCGCGGCCGCGCAGATAAATTTACTT and reverse primer *F GCGCGCTCTAGAGGAATTCGTGTGTTTTAT, cloned into TOPO TA and shuttled into *F P{Express-UAS}. *F P{UAS-arm.Exel}1 is an X chromosome insertion. *F P{UAS-arm.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-arm.Exel}3 is a third chromosome insertion. *F In P{GMR-arm.Exel}, wild type arm was cloned into P{Express-glass}. The arm *F sequence was PCR amplified using forward primer *F GCGCGCGCGGCCGCGCAGATAAATTTACTT and reverse primer *F GCGCGCTCTAGAGGAATTCGTGTGTTTTAT, cloned into TOPO TA and shuttled into *F P{Express-glass}. *F P{GMR-arm.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{vgMQ-arm.Ndel}, the arm gene deleted for the first 128 amino acids was *F cloned into P{Express-vgMQ}. The encoded mutant protein is constitutively *F active. *F P{vgMQ-arm.Ndel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{vgMQ-arm.Ndel}2 is a second chromosome insertion. *F P{vgMQ-arm.Ndel}3 is a third chromosome insertion. *F In P{GMR-arm.S56F}, arm sequences encoding a S56F variant protein were *F cloned into P{Express-glass}. The mutant protein is constitutively *F active. The S56F change mimics the S45F change in humans. *F P{GMR-arm.S56F}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-arm.S56F}2 is a second chromosome insertion *F P{GMR-arm.S56F}3 is a third chromosome insertion. *F In P{GMR-arm.T52A}, arm sequences encoding a T52A variant protein were *F cloned into P{Express-glass}. The mutant protein is constitutively *F active. The T52A change mimics the T41A change in humans. *F P{GMR-arm.T52A}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178865 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u Eaat1 constructs and insertions. *F Date: Wed, 12 May 2004 15:18:23 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: Eaat1 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F P{UAS-Eaat1.Exel} consists of the wild type Eaat1 (FBgn0026439) sequence *F cloned into P{Express-UAS}. *F P{elav-Eaat1.Exel} consists of the wild type Eaat1 sequence cloned into *F P{Express-elav}. *F P{elav-Eaat1.Exel}3 a homozygous viable and fertile, third chromosome *F insertion. *F P{UAS-Eaat1.Exel}3 is a third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178866 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u wfs1 constructs and insertions. *F Date: Wed, 12 May 2004 15:19:38 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: wfs1 constructs and insertions *F The following information was provided by Annette Parks of Exelixis, Inc. *F CG4917 (FBgn0039003) is the fly homolog of the human Wolfram syndrome 1 *F (WFS1) gene. The human protein is also called wolframin in some references. *F In P{GMR-wfs1.Exel}, full length wfs1 was cloned into *F P{Express-glass}. The sequence of wfs1 in this construct and the two *F others below is not identical to other sequenced clones. Its sequence is: *F MATWTQNEPTGVTKRRRWNLEDRASLNKLKHHIAEEGCPQMQYDLAKELLDNSIEPNLAKGNQSQKAVNWLVSAAHNGH *F EDAVKLLRQCYNDGSGITAENTDEVRRCLAMTPGERAARKAARELFACLSNGNEHITPKQLERKMRRIYNLQRKRRRRD *F DDRSSSSSEGEQEPECEPLEDVPTIDLANVERRRLITEAHLVSAASNYSAGQMPSVNDALTLSVPDPRSLDHVPCFYRM *F IFHPLIFFTLFYHRLLNLIVSIPNVIPLSVRCSVLVAISWWSSRHMLPLVSYYLSLGIMIWATCKMLKTKQQFVDFRIW *F SGLFLSYGDQNIEADIAEHRFLRNNMKPYLYFFCAFICNLIVYPLVTDAWLPHSELTIISGALTFITMCVSMYASSHQL *F PDWLVIVSFAVNVLAKYPYEMDEVVSTRWRFLDLRVPTFSSFVIGNGIEFCLNCRTALYLFIPVLLIMMAKRSRWHGVY *F TFLIPHCVTLSWLQVCIATSQSSTVFGVMRAALGLAGIVLFLPLFGIVALLVPVFVAIDSLGLASEQLRWGSTALACGL *F VVVLSCILALNRATQKYITMLQLITAITTACLLVLPYMTSSFKDTPRFNAMPRAGLHSLSETNTLPWDRFHALCAQPVH *F EQPNKIKAQLRCSLLNGMPVIWEGSVTKVEISRVSNFLEDTIANYLPVWLGRMLRCLHGENISQHFKCDPKLDAQCEEW *F RSVFKTFNAQSGSCTLQRWNRYEYELLVKVGTKRSGRLLGRSTTTDVILRAHHDFGNFTRLLSEGDVVLFYGILHNSRL *F LADNVQVKLKTIECVECRSRDLGTASIERVVAASPMDARLQDLMRGIKYLLNALLNPLITFK *F P{GMR-wfs1.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{GMR-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{UAS-wfs1.Exel}, full length wfs1 was cloned into P{Express-UAS}. *F P{UAS-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{sev-wfs1.Exel}, full length wfs1 was cloned into either *F P{Express-sev1x} or P{Express-sev3x}. *F P{sev-wfs1.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{sev-wfs1.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{sev-wfs1.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178867 *a Parks *b A. *t 2004.5.12 *T personal communication to FlyBase *u PBac{PB}9B, PBac{RB}3AR and PBac{WH}3AWS. *F Date: Wed, 12 May 2004 15:24:47 -0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu, Annette Parks *F *F From: Kevin Cook *F Subject: PBac{PB}9B, PBac{RB}3AR and PBac{WH}3AWS *F *F The following information was provided by Annette Parks of Exelixis, Inc. *F *F PBac{PB}9B is a homozygous and hemizygous viable and fertile, X chromosome *F insertion. *F *F PBac{RB}3AR is an X chromosome insertion. *F *F PBac{WH}3AWS is an insertion on Binsinscy (In(1)sc[S1L]sc[8R]+dl-49; *F FBab0010488). *F *F These three insertions were used as starting insertions in transposition *F screens described in Thibault et al., 2004 (Nature Genetics 36: 283-287). *F *F *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F ------------------------------------------------------------------------ # *U FBrf0178868 *a Parks *b A. *t 2004.5.17 *T personal communication to FlyBase *u P{Rbf-FRT.2.1} construct and insertions. *F Date: Mon, 17 May 2004 13:57:34 \-0500 *F To: flybase-updates@morgan.harvard.edu, rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{Rbf-FRT.2.1} construct and insertions *F Cc: 'Annette Parks':; *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{Rbf-FRT.2.1}, the Rbf (FBgn0015799) coding sequence and regulatory *F region were cloned into P{Express-FRT.2.1}. Upon exposure to FLP *F recombinase, the Rbf gene and miniwhite are excised from the construct. *F P{Rbf-FRT.2.1}2 is a homozygous viable and fertile, second chromosome insertion. *F P{Rbf-FRT.2.1}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178869 *a Parks *b A. *t 2004.5.17 *T personal communication to FlyBase *u p53 constructs and insertions. *F Date: Mon, 17 May 2004 14:07:28 \-0500 *F To: rd120@gen.cam.ac.uk, flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: p53 constructs and insertions *F Cc: 'Annette Parks':; *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{vgMQ-p53.Exel}, the wild type p53 (FBgn0039044) coding sequence was *F cloned into P{Express-vgMQ}. *F P{vgMQ-p53.Exel}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{vgMQ-p53.Exel}2 is a second chromosome insertion. *F P{vgMQ-p53.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F In P{sev-p53.Exel}, the wild type p53 coding sequence was cloned into *F either P{Express-sev1x} or P{Express-sev3x}. *F P{sev-p53.Exel}1 a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{sev-p53.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{sev-p53.Exel}3 is a third chromosome insertion. *F In P{ey3.5-p53.Exel}, the wild type p53 coding sequence was cloned into *F P{Express-ey3.5}. *F P{ey3.5-p53.Exel}2 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178870 *a Parks *b A. *t 2004.5.17 *T personal communication to FlyBase *u Delta2-3 constructs and insertions. *F Date: Mon, 17 May 2004 14:41:13 \-0500 *F To: flybase-updates@morgan.harvard.edu, rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Delta2-3 constructs and insertions *F Cc: 'Annette Parks':; *F The following information was provided by Annette Parks of Exelixis, Inc. *F In PBac{Delta2-3.Exel}, the Delta2-3 sequence was cloned into a piggyBac *F vector. *F PBac{Delta2-3.Exel}106 is a homozygous viable and fertile, second *F chromosome insertion. *F PBac{Delta2-3.Exel}2 is an insertion on CyO. The Bloomington Stock Center *F will call this balancer variant CyO, PBac{w+mC=Delta2-3.Exel}2 in its *F stock list. *F In PBac{GMR-Delta2-3}, the Delta2-3 sequence under the control of GMR was *F cloned into a piggyBac vector. *F PBac{GMR-Delta2-3}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178871 *a Deal *b J. *c K. *d Cook *t 2004.5.27 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC50. *F Date: Thu, 27 May 2004 15:39:54 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC50 *F Isolation and characterization of Df(2L)BSC50 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC50 was isolated as a P transposase-induced male recombination *F event involving P{EP}CG13130EP2238 and P{EPgy2}EY03684. The deletion was *F isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 *F cn1 bw1 females x P{EP}CG13130EP2238 cn1/dpov1 P{P{EPgy2}EY03684; *F TMS, P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 30F4-5;31B1-4. Df(2L)BSC50 failed to complement bib1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178872 *a Deal *b J. *c K. *d Cook *t 2004.5.27 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC51. *F Date: Thu, 27 May 2004 15:42:35 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC51 *F Isolation and characterization of Df(2L)BSC51 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC51 was isolated as a P transposase-induced male recombination *F event involving P{GT1}CG15636BG01429 and P{GT1}BG01694. The deletion was *F isolated as a net1-cn1 recombinant chromosome from the cross net1 *F b1 cn1 sp1 females x net1 P{GT1}CG15636BG01429/P{GT1}BG01694 *F cn1; TMS, P{Delta2-3}99B/+ males. Polytene chromosome squashes showed *F the breakpoints 25A2-3;25C2-5. Df(2L)BSC51 failed to complement *F vkg01209, eIF-3p40k09003 and betaggt-IS-2554. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178873 *a Deal *b J. *c K. *d Cook *t 2004.5.27 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC52. *F Date: Thu, 27 May 2004 15:44:24 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC52 *F Isolation and characterization of Df(2L)BSC52 *F Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(2L)BSC52 was isolated as a P transposase-induced male recombination *F event involving P{GT1}CG15636BG01429 and P{PZ}l(2)0571405714. The *F deletion was isolated as a net1-cn1 recombinant chromosome from the *F cross net1 b1 cn1 sp1 females x net1 *F P{GT1}CG15636BG01429/P{PZ}l(2)0571405714 cn1; TMS,P{Delta2-3}99B/+ *F males. Polytene chromosome squashes showed the breakpoints *F 25A1-3;25B6-8. Df(2L)BSC52 failed to complement betaggt-IS-2554. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178874 *a Featherstone *b D. *c K. *d Cook *t 2004.5.18 *T personal communication to FlyBase *u Breakpoints of Df(3R)H-B79. *F Date: Tue, 18 May 2004 16:24:50 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F comments: As part of a mapping project, we've used PCR to *F improve the localization of the breakpoints in Df(3R)H-B79. *F Since this Df stock is at Bloomington and is one of only a few *F in this region, I thought the info might be useful to others. *F Our data shows that the left breakpoint of Df(3R)H-B79 is *F between 92E8 and 92E9, near base 16272000 (just right of *F CG17208). The right breakpoint is between 92E12 and 92E13, *F near base 16330000 (just left of CG4323). We used primers *F designed to amplify specific genomic sequences and looked in *F homozygous Df embryos for (lack of) PCR product. *F realname: Dave Featherstone *F reply-to: def@uic.edu *F source: FB-NG Help Mail: *F usersubject: breakpoints of Df(3R)H-B79 *F \-------------------------------------------------------------------- *F Date: Thu, 20 May 2004 15:01:00 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F To: flybase-help@morgan.harvard.edu, def@uic.edu *F Dear Dave, *F thanks for this info \- so's I can record it for best use I need to be *F able to delimit which genes are deleted as well as those that are not. *F To confirm, in this case there is only one gene deleted, it being *F CG5115. Is that correct? *F Thanks for help, *F Rachel *F for FlyBase curators. *F \-------------------------------------------------------------------- *F From: Dave Featherstone *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F Date: Thu, 20 May 2004 10:46:48 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F Yes, CG5115 is deleted, but CG4323 may also be. *F \-Dave *F \-------------------------------------------------------------------- *F Date: Tue, 18 May 2004 18:09:54 \-0500 (EST) *F From: Kathy Matthews *F Reply-To: Kathy Matthews *F Subject: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F To: kcook@bio.indiana.edu *F Cc: matthewk@fly.bio.indiana.edu *F Kevin, *F This is a DK3 (#4962). His results are very different *F from current bps (92B;92F13) and inconsistent with reports *F of failure to complement H at 92F3, bon at 92F2-3 and cdc2c *F at 92F10. Do you have any data for this one? *F \-- K *F \-------------------------------------------------------------------- *F Date: Thu, 20 May 2004 13:06:44 \-0500 *F To: matthewk@indiana.edu, rd120@gen.cam.ac.uk, def@uic.edu *F From: Kevin Cook *F Subject: Fwd: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F Hi Dave-- *F I just did a polytene chromosome prep from our Df(3R)H-B79 stock, and I saw *F the same breakpoints that were reported in the literature. I didn't take *F the time to reanalyze the cytology down to single bands, but I confirmed *F that the proximal breakpoint is somewhere in 92B and the distal breakpoint *F is somewhere in 92F. *F I don't know how to reconcile this with your PCR results. Maybe the stock *F carries all or a portion of 92BF transposed to another region of the *F genome. A transposition could fail to complement H, bon, *F cdc2c, l(3)10585, Stat92E and CG17838, as reported, if these genes were *F subject to position effect variegation. I didn't look for an insertion of *F 92BE in the squashes, since I was looking at chromosomes from H-B79/TM2 *F larvae and the balancer makes this really hard to do. *F I'm reluctant to list this stock as a Tp instead of a Df without some other *F evidence. *F Best regards, *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F \-------------------------------------------------------------------- *F Cc: matthewk@bio.indiana.edu *F From: Dave Featherstone *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F Date: Thu, 20 May 2004 19:03:08 \-0500 *F To: Kevin Cook *F Hi Kevin, *F All I can say is what our (my Tech's) PCR results were, which is what I *F forwarded via email. Chris Rodesch also did come complementation tests *F with H-B79 and said it complemented Stat92E, which is consistent with *F our PCR data (Although I later did this cross and concluded that B79 *F failed to complement Stat92E). Other than that, I can tell you *F nothing. We are unlikely to figure anything more out here since the *F H-B79 PCR was done while searching for a mutation that subsequently *F turned out to be in 92F4. Plus, I too hate TM2. *F I wrote because I thought our results might be handy additions to the *F annotation for H-B79, especially since they suggest the Df is smaller. *F I won't be offended at all if my note is associated with an 'editorial *F comment' noting that our PCR results don't mesh with other work and may *F be bogus. At least future researchers will have a heads up to look for *F themselves, hopefully resolve the matter, and write to Flybase. *F Anyway, it's your call. You guys do great work. *F \-Dave *F David E. Featherstone *F Department of Biological Sciences *F University of Illinois at Chicago *F SEL East, Room 4311 (Corner of Taylor & Halsted) *F 840 W. Taylor St. (M/C 067) *F Chicago, IL 60607 *F Phone: (312) 413-2516 *F FAX: (312) 996-2805 *F Email: def@uic.edu *F http://tigger.uic.edu/~def/ *F \-------------------------------------------------------------------- *F Date: Fri, 21 May 2004 12:19:17 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F To: def@uic.edu, flybase-help@morgan.harvard.edu, kcook@bio.indiana.edu *F Dear all, *F This is all a bit odd as the PCR assessments would say that Df(3R)H-B79 *F doesn't even delete H. H is at coordinate 16447K or so and CG5115 (the *F only gene to be wholly deleted, according to the PCR data) is at *F coordinate 16309K or so. Stat92E is at 166371K or so. *F It is possible for the FlyBase aberrations data file to house *F statements about what is molecularly deleted and not deleted without *F these causing adjustments to statements about cytologically determined *F breakpoints. *F So I will curate your disussion as a pers comm from Dave and Kevin, and *F capture *F a. apparently contradictory data *F b. discussion of possibility of explanation \- and possibility of Tp. *F If there is to be confusion it is as well to document it well! *F . *F . *F best to all, *F Rachel. *F \-------------------------------------------------------------------- *F From: Dave Featherstone *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F Date: Fri, 21 May 2004 08:20:19 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F Sounds fine to me. *F The PCR was done on the stock recently acquired from Bloomington. *F Chris Rodesch's comp tests were done when we were both in Kendal *F Broadie's lab in Utah, so used a previously-acquired stock. *F \-Dave *F \-------------------------------------------------------------------- *F Date: Fri, 21 May 2004 09:41:59 \-0500 *F To: 'Rachel Drysdale (Genetics)' , def@uic.edu, *F flybase-help@morgan.harvard.edu *F From: Kevin Cook *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F Hi Rachel-- *F It's fine with me to archive this discussion. *F The H-B79 stock does show a Hairless phenotype. Specifically, postvertical *F and orbital bristles are missing and wing vein L5 thins out as it *F approaches the wing margin. *F Thanks! *F Kevin *F \-------------------------------------------------------------------- *F Cc: 'Rachel Drysdale (Genetics)' , *F flybase-help@morgan.harvard.edu *F From: Dave Featherstone *F Subject: Re: FB-NG Help Mail: breakpoints of Df(3R)H-B79 *F Date: Fri, 21 May 2004 10:46:34 \-0500 *F To: Kevin Cook *F For what it's worth: I just checked our Df(3R)H-B79 stock, on which the *F PCR was performed, and it also is Hairless. And that, I agree, runs *F counter to the fact that we got PCR product from primers designed to *F amplify near base 16329k. *F \-Dave *F \-------------------------------------------------------------------- # *U FBrf0178875 *a Dickson *b B. *t 2004.6.1 *T personal communication to FlyBase *u *F Date: Tue, 01 Jun 2004 13:11:31 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{sE-raftorY9}475 insertion *F The following information was provided by Barry Dickson, University of *F Vienna (6/04). *F P{sE-raftorY9}475 is an insertion on CyO described in Dickson et al., *F Genetics 142: 163-171, 1996 (FBrf0086382). *F This balancer variant will be called CyO, P{w+mW.hs=sE-raftorY9}475 in *F the Bloomington stock list. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178876 *a Bloomington Drosophila Stock Center *b ?. *t 2004.6.3 *T personal communication to FlyBase *u *F Date: Thu, 3 Jun 2004 18:52:10 \-0500 (EST) *F From: Kathy Matthews *F Subject: Subject: P{dppshv-lacZ.RD2} *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: P{dppshv-lacZ.RD2} *F Dated: 3 June 2004 *F P{dppshv-lacZ.RD2}RD2 is a viable and fertile insertion into *F chromosome 3. It was generated in the Gelbart laboratory. *F Heuer et al., 1995 (FBrf0084021), incorrectly states that *F P{dppshv-lacZ.RD2} carries w+mC. According to Hursh et al., *F 1993 (FBrf0058069), P{dppshv-lacZ.RD2} was generated from *F pP{HZ50PL} and carries ry+t7.2. # *U FBrf0178877 *a Pilling *b A. *c W. *d Saxton *t 2004.6.16 *T personal communication to FlyBase *u *F Date: Wed, 16 Jun 2004 12:38:36 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{mitoGFP.AP} construct and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Aaron Pilling and Bill Saxton, Indiana University (4/04). *F The P{mitoGFP.AP} construct encodes the 31 amino acid mitochondrial import *F sequence from human cytochrome C oxidase subunit VIII fused to the N *F terminus of the S65T spectral variant of GFP. The original chimeric *F protein was described in Rizzuto et al., 1995 (Current Biology 5: 635-642). *F The chimeric DNA fragment was cloned into pP{UAST}. *F P{mitoGFP.AP}2 is a second chromosome insertion. *F P{mitoGFP.AP}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178878 *a Laudet *b V. *t 2004.6.14 *T personal communication to FlyBase *u *F Date: Mon, 14 Jun 2004 15:41:01 \+0200 *F From: Vincent Laudet *F To: flybase-updates@morgan.harvard.edu *F Subject: update on nuclear hormone receptor nomenclature *F Dear curators of The FlyBase, *F My lab maintains the official nomenclature of nuclear hormone receptors *F (Cell 97: 1-20). We have recently done an important effort to re *F evaluate the nuclear receptor content of published animal genomes (to *F appear in Mol Biol Evol). In this process, we have noticed some small *F discrepancies between the nomenclature we use and that used in FlyBase. *F We would like to suggest that you add the following names as synonyms. *F Thank you for the wonderful job you do in maintaining FlyBase. *F flybase ID flybase name suggested synonyms *F FBgn0034012 CG16801 PNR, NR2E3 *F FBgn0037436 CG10296 FAX-1, NR2E5 *F FBgn0023546 Hr4 GRF, NR6A2 *F Sincerely yours, *F Vincent Laudet *F \-- *F Pr. Vincent Laudet *F Membre de l'Institut Universitaire de France *F Structure and Evolution of Nuclear Hormone Receptors *F UMR 5161 du CNRS, INRA LA 1237 *F Laboratoire de Biologie Moléculaire de la Cellule *F IFR128 BioSciences Lyon-Gerlan *F Ecole Normale Supérieure de Lyon *F 46 Allée d'Italie *F 69364 Lyon Cedex 07 *F France *F Tel : 33 (0)4 72 72 81 90 *F Portable : 33 (0)6 16 41 73 34 *F Fax : 33 (0)4 72 72 80 80 *F e-mail : Vincent.Laudet@ens-lyon.fr # *U FBrf0178879 *a Levis *b R. *t 2003.4.28 *T personal communication to FlyBase *u Corrected location for EY01148. *F Date: Mon, 28 Apr 2003 23:18:35 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: Corrected location for EY01148 *F Cc: , 'Roger A. Hoskins' *F , Joe Carlson *F I am writing on behalf of the BDGP Gene Disruption Project to correct *F an error in the insertion site position for the P-element in the *F EY01148 mutant. The FlyBase record has the insertion site in *F 3R-87B14 associated with the gene Hsp70Bbb. I assume that this site *F was based on the insertion site data supplied by our Project, which *F mapped it by aligning the flanking sequence with the genome sequence, *F probably at a time when only release 2 of the genome sequence was *F available. I have reexamined the alignment of the EY01148 flanking *F sequence with release 3 of the genome sequence, as well as other *F GenBank sequence records (most notably the record with accession *F J01103.1 GI:157710). The best alignment of the EY01148 flanking *F sequence is at AE003693.3 coordinate 207312 (plus strand) = arm_3R *F coordinate 7784134, which corresponds to estimated cytogenetic region *F 87A3-7, between the Hsp70Aa and Hsp70Ab genes. The insertion is 0.16 *F kb from the annotated 5' end of the Hsp70Ab gene. There is a gap in *F the alignment of the EY01148 with the genome sequence in this region *F that probably corresponds to a S-element that is annotated in the *F BDGP/Celera genome sequence. This S-element appears to be *F polymorphic, as it is absent in the genome sequence in GenBank record *F J01103. The mapping of the EY01148 flanking sequence was complicated *F both by this S-element and by the similarity of the sequences present *F upstream of the reiterated copies of the Hsp70 genes. *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 115 West University Parkway *F Baltimore, MD 21210, U.S.A. *F phone: 1-(410) 554-1238 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0178880 *a Irvine *b K. *t 2004.6.25 *T personal communication to FlyBase *u *F Date: Fri, 25 Jun 2004 15:17:32 \-0500 (EST) *F From: Kathy Matthews *F Subject: Insertions of fringe construct *F To: flybase-updates@morgan.harvard.edu *F Cc: irvine@waksman.rutgers.edu, matthewk@fly.bio.indiana.edu *F Personal communication from: Ken Irvine, Rutgers, The State University of New *F Jersey *F To: The Bloomington Drosophila Stock Center *F Subject: Insertions of fringe construct *F Dated: 16 June 2004 *F Background: Some additional information from Ken Irvine about an insertion *F already in the Bloomington collection has led to a symbol change. He also *F provided information on two additional insertions that are not yet in FlyBase. *F New valid symbol for existing insertion: *F FBti Valid Symbol Synonyms Synonym Comment *F FBti0015591 P{UAS-fng.K}27 UAS-fng27 This is the symbol used by the *F Irvine lab *F P{UAS-fng.K}JK1 This is the symbol that was used *F at the stock center between *F 5/18/2000 and 6/16/2004. *F New insertions (new to FlyBase): *F Symbol Synonym Phenotype and location *F P{UAS-fng.K}22A UAS-fng22A viable, fertile insertion on chromosome 2 *F P{UAS-fng.K}22B UAS-fng22B viable, fertile insertion on chromosome 2 *F Comment from K. Irvine: 'The original UAS-fng22 stock had multiple *F insertions on the second chromosome \- we later sorted them out into two *F single insertions, which we called UAS-fng22A and UAS-fng22B, and a *F chromosome with both insertions, which we call UAS-fng22C.' # *U FBrf0178881 *a Roote *b J. *t 2004.6.24 *T personal communication to FlyBase *u ED genetic data. *F From: John Roote *F Subject: ED genetic data *F Date: Thu, 24 Jun 2004 11:50:02 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F Data regarding the EDs in the M(3)86D region: *F Df(3R)ED5506 Minute+ cu+ *F Df(3R)ED5511 Minute- cu- *F Df(3R)ED5514 Minute- cu- *F Df(3R)ED5518 Minute- cu- *F Df(3R)ED5516 Minute+ cu+ *F (cu phenotype scored when heterozygous with TM6C, cu Sb e ca) *F This places both curled and a Minute in the interval 6998488..7059910. *F RpL3 and RpS25 are in this interval. *F Various: *F Df(3R)ED5429 dmt- (data from Bilal Kerman) *F Df(1)ED7374 if- does not complement if3) *F Df(1)ED429 homozygous viable, female sterile *F Df(1)ED7008 homozygous viable, female sterile *F Df(1)ED7006 homozygous viable, female sterile *F Df(2L)ED1454 homozygous viable *F Df(2L)ED1050 stc-, gft-, ms(2)35Ci-, l(2)35Bf-, lethal over Df(2L)rd9 *F Df(3L)ED4079 homozygous viable *F Df(4)ED6369 suppresses phenotype of eyD *F Df(4)ED6366 Minute+ pan- *F Df(4)ED6364 Minute- (ED predicted to delete RpS3A) pan- *F J # *U FBrf0178882 *a Roote *b J. *t 2004.6.24 *T personal communication to FlyBase *u *F To: Rachel Drysdale (Genetics) *F Cc: Kevin Cook , David Gubb *F Subject: Cam Dps *F From: John Roote *F Date: Thu, 17 Jun 2004 11:46:41 \+0100 *F Hi Rach, *F I am attaching a file containing data on the new cytological orders of *F the so-called Cam duplications. Please do not read anything into the *F 2Lh, 2Rh, etc. designations. I am not presenting new data here. The *F only breakpoints which have been confirmed or for which there are new *F data are the ones which directly affect the duplications. *F John *F The Cam duplication set *F New cytological order *F Duplicated segment *F Abbreviation *F Genetic designation *F 2Lt \- 23D1|2Rh \- 21E2|41F \- 2Rt *F 21E2;23D1  *F Dp(2;2)Cam1  *F In(2LR)DTD8LS325R *F 2Lt \- 26C1.2|2Rh \- 23D1|2Rh \- 2Rt  *F 23D1;26C1.2   *F Dp(2;2)Cam2  *F In(2LR)DTD24LDTD8R *F 2Lt \- 29E|41A-C \- 26C1.2|2Rh \- 2Rt  *F 26C1.2;29E *F Dp(2;2)Cam3  *F In(2LR)DTD111LDTD24R, bw sp *F 2Lt \- 27D|41A-B \- 26C|2Rh \- 2Rt *F 26C;27D *F Dp(2;2)Cam3a *F In(2LR)DTD51LDTD24R, bw sp *F 2Lt \- 29F|41A-C \- 27F|2Rh \- 2Rt *F 27F;29F *F Dp(2;2)Cam3b *F In(2LR)DTD111LDTD11R, (sp) *F 2Lt \- 27F|2Rh \- 27C|2Rh \- 2Rt *F 27C;27F *F Dp(2;2)Cam3c  *F In(2LR)DTD11LDTD51R bw *F 2Lt \- 23D-E|28D \- 23D-E|28D \- 32F|2Rh \- 29F|41A-C \- 2Rt  *F 29F;32F  *F Dp(2;2)Cam4 *F In(2LR)DTD107LDTD111R \+ In(2L)DTD107, TE23CD vg *F 2Lt \- 35B1.2|2Rh \- 32F|2Rh \- 2Rt *F 32F;35B1.2 *F Dp(2;2)Cam5 *F In(2LR)noc-4LDTD107R, b *F 2Lt \- 36C|2Rh \- 35B1.2|2Rh \- 2Rt *F 35B1.2;36C *F Dp(2;2)Cam6  *F In(2LR)S1Lnoc-4R *F 2Lt \- 2Lh|2Rh \- 36C|2Rh \- 2Rt *F 36C;2Lh  *F Dp(2;2)Cam8 *F In(2LR)C3LCH9-25R, b *F 2Lt \- 35B1.2|43A1.2 \- 35B1.2|2Rh \- 2Rt *F 2Rh;43A1.2 *F Dp(2;2)Cam10 *F In(2LR)TE35B-4L noc-4R, (al) dp b cn bw *F 2Lt \- 35B1.2|47B10-14 \- 35B1.2|43A1.2 \- 2Rt *F 43A1.2;47B10-14  *F Dp(2;2)Cam11 *F In(2LR)TE35B-226L TE35B-4R, b pr l(2)pwn *F 2Lt \- 35B1.2|48C \- 35B1.2|47B10-14 \- 2Rt *F 47B10-14;48C *F Dp(2;2)Cam13 *F In(2LR)DTD128LTE35B-226R, net ho2 TE23CD *F 2Lt \- 2Lh|48B1.2 \- 2Lh|52D5 \- 2Rt *F 48B1.2;52D5  *F Dp(2;2)Cam12 *F In(2LR)RevBL P{lacW}k06524R *F Note: construction proceeds through DS autosynaptic element *F intermediate generated from P{lacW}k06524 by Delta2-3 induced *F recombination between insertion site and 2L heterochromatin. *F 2Lt \- 51EF|3Rh \- 3Rt; 3Lt \- 3Rh|49A \- 2Rt *F 49A;51EF *F Dp(2;3)Cam14T  *F Ts(2Lt;3Rt)6r23 \+ Ts(2Rt;3Lt)H24, cn *F 2Lt \- 2Lh|52D5 \- 2Lh|51F11-12 \- 2Rt *F 51F11-12;52D5 *F Dp(2;2)Cam12a  *F In(2LR)RevBL P{lacW}k03308R *F Note: construction proceeds through DS autosynaptic element *F intermediate generated from P{lacW}k03308 by Delta2-3 induced *F recombination between insertion site and 2L heterochromatin. *F 2Lt \- 40B-F|57C4-6 \- 2Lh|52D5 \- 2Rt *F 52D5;57C4-6 *F Dp(2;2)Cam15 *F In(2LR)Pu-LL RevBR, cn bw *F 2Lt \- 40F|53F1-4 \- 2Lh|52D5 \- 2Rt *F 52D5;53F1-4 *F Dp(2;2)Cam17 *F In(2LR)DTD99L RevBR, cn bw *F 2Lt \- 40B-F|57C4-6 \- 2Lh|53F1-4 \- 2Rt  *F 53F1-4;57C4-6 *F Dp(2;2)Cam18 *F In(2LR)Pu-LLDTD99R *F 2Lt \- 2Lh|60E5-8 \- 2Lh|57C4-6 \- 2Rt *F 57C4-6;60E5-8  *F Dp(2;2)Cam16  *F In(2LR)lt-G16LPu-LR, b bw *F 3Lt \- 64B10-12|81F \- 61F7-8|82A1 \- 3Rt *F 61F7-8;64B10-12 *F Dp(3;3)Cam31 *F In(3LR)P93LLD3R, pp *F 3Lt \- 64E5|81F-82A \- 64B10-12|81F \- 3Rt  *F 64B10-12;64E5  *F Dp(3;3)Cam32  *F In(3LR)LD12LP93R *F 3Lt \- 65C5-9|3Rh \- 64E5|81F-82A \- 3Rt *F 64E5;65C5-9 *F Dp(3;3)Cam33 *F In(3LR)P10LLD12R, Ubxbx43e *F 3Lt \- 67C5-9|81F \- 65C5-9|3Rh \- 3Rt  *F 65C5-9;67C5-11  *F Dp(3;3)Cam34 *F In(3LR)LD31LP10R, mwh *F 3Lt \- 69A4-5|81F \- 67C5-9|81F \- 3Rt  *F 67C5-11;69A4-5 *F Dp(3;3)Cam35 *F In(3LR)274LLD31R, Ubxbx43e *F 3Lt \- 70F1-2|81F1 \- 69A4-5|81F \- 3Rt  *F 69A4-5;70F1-2 *F Dp(3;3)Cam36 *F In(3LR)P42L274R, ri in cu sr es ca *F 3Lt \- 73C7|81 \- 70F1-2|81F1 \- 3Rt *F 70F1-2;73C7  *F Dp(3;3)Cam37  *F In(3LR)ME24LP42R *F 2Lt \- 35A4-B1|93C \- 3Lt; 3Rt \- 90C|35B1.2 \- 2Rt *F 90C;93C  *F Dp(3;3)Cam30T *F Ts(2Lt;3Lt)el-24 \+ Ts(2Rt;3Rt)TE35B-28, pr l(2)pwn cn *F 2Lt \- 2Lh|2Rh \- 36C|2Rh \- 2Rt *F 36C;2Lh *F Dp(2;2)Cam8a *F In(2LR)D29LCH9-25R, b # *U FBrf0178883 *a Dura *b J.M. *t 2004.7.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Jul 2004 15:01:58 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Src64B.C}2 insertion *F A stock with the following insertion was obtained from Jean-Maurice *F Dura. The construct was made and transformed in the lab of Jonathan *F Cooper. The insertion was mapped to a chromosome here at the Bloomington *F Stock Center. *F P{UAS-Src64B.C}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178884 *a Dura *b J.M. *t 2004.7.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Jul 2004 15:04:07 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-plexA.W}3 *F A stock with the following insertion was obtained from Jean-Maurice *F Dura. Its construction and transformation were described in *F FBrf0105958. The insertion was mapped to a chromosome here at the *F Bloomington Stock Center. *F P{UAS-plexA.W}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178885 *a Bellen *b H. *t 2004.7.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Jul 2004 15:18:03 \-0500 *F To: FlyBase updates *F From: Kevin Cook *F Subject: Df(1)v-M13-E7 *F Df(1)v-M13-E7 was donated to the Bloomington Stock Center by Wouter Ferro *F of the Department of Radiation Genetics and Chemical Mutagenesis of the *F Leiden University Medical Center. Accompanying notes said that it was *F generated in the Amherst M56i strain, isolated over the balancer M6 and *F initially called l(1)vM13-E7 and Df(1)vM13-E7. *F My polytene analysis showed the breakpoints 9D2-E1;9F2-10A1. *F From the genetic background, balancer and the source of the stock, it's *F likely that this deletion was isolated in the studies described in Eeken et *F al., 1994, 'The nature of X-ray-induced mutations in mature sperm and *F spermatogonial cells of Drosophila melanogaster' Mutation Research 307: *F 201-212 (FBrf0073021). If so, it probably corresponds to Df(1)v-Ee6 *F (FBab0026968), Df(1)v-Ee7 (FBab0026969), Df(1)v-Ee8 (FBab0026970), *F Df(1)v-Ee9 (FBab0026971) or Df(1)v-Ee31 (FBab0026976), based on the *F similarity of reported and observed breakpoints. *F Df(1)v-M13-E7 fails to complement vOf. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178887 *a Wang *b Z. *c R. *d Mann *t 2004.7.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Jul 2004 20:58:32 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{vis}3, P{achi}3 and P{tub-achi.L}3 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Zhaohui Wang and Richard Mann, Columbia University (5/04). *F P{achi}3 is a homozygous viable and fertile, third chromosome insertion. *F P{vis}3 and P{tub-achi.L}3 are third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178888 *a Desplan *b C. *t 2004.7.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Jul 2004 21:17:19 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-hb.W}F4A and P{UAS-hb.W}F1A insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Claude Desplan, New York University (5/04). *F P{UAS-hb.W}F4A is a homozygous viable and fertile, second chromosome insertion. *F P{UAS-hb.W}F1A is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178889 *a Chihara *b C. *t 2004.7.8 *T personal communication to FlyBase *u Lcp65Ab1 mutations. *F Date: Thu, 08 Jul 2004 10:12:54 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Lcp65Ab1 mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Carol Chihara, University of San Francisco (5/04). *F Lcp10Rho (FBal0011584) is an allele of Lcp5 which has a serine in place *F of proline in mature protein position 2 (after the signal sequence is *F removed). Lcp5 is encoded by Lcp65Ab1 and Lcp65Ab2, but the mutation has *F not been mapped to either specific gene. (To be consistent with the *F current Lcp65Ab1 and Lcp65Ab2 entries, this mutation should be arbitrarily *F assigned to Lcp65Ab1 and called Lcp65Ab1Rho with the understanding that *F it could be in Lcp65Ab2. The Lcp10 gene entry (FBgn0002540) should be *F merged with the entry for Lcp65Ab1 (FBgn0020644) and cross-referenced to *F the entry for Lcp65Ab2 (FBgn0020643).) *F Lcp65Ab1op is probably a duplication. *F In Lcp65Ab1Fnr, either Lcp65Ab1 and Lcp65Ab2 encode the same protein *F variant, or one gene is inactive and the other is modified. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178890 *a Romeijn *b R. *c W. *d Ferro *t 2004.7.8 *T personal communication to FlyBase *u ligase4 mutations. *F Date: Thu, 08 Jul 2004 11:09:29 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: ligase4 mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Ron Romeijn and Wouter Ferro of the Leiden University *F Medical Center (6/04). *F ligase45, ligase457 and ligase429 are mutations isolated from *F imprecise excision of P{EP}ligase4EP385. All are homozygous and *F hemizygous viable and fertile. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178891 *a Chang *b H. *t 2004.6 *T personal communication to FlyBase *u Chang constructs and insertions. *F Date: Thu, 08 Jul 2004 12:06:00 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Chang constructs and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Henry Chang, Yale University (6/04). *F In P{UAS-Rab4-mRFP}, the Rab4 (=CG4921) coding sequence is fused to the *F mRFP sequence. The fusion protein labels early endosomes. *F P{UAS-Rab4-mRFP}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{UAS-Rab11-GFP}, the Rab11 (=CG5771) coding sequence is fused to the *F EGFP coding sequence. The fusion protein labels recycling endosomes. *F P{UAS-Rab11-GFP}2 is a homozygous viable and fertile, second chromosome *F insertion. *F In P{UAS-Grasp65-GFP}, the CG7809 coding sequence is fused to the EGFP *F coding sequence. The fusion protein labels Golgi complex. *F P{UAS-Grasp65-GFP}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Grasp65-GFP}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178892 *a Ratnakumar *b K. *c C. *d Desplan *t 2004.7.8 *T personal communication to FlyBase *u P{Rh4-lacZ.PD} construct and insertions. *F Date: Thu, 08 Jul 2004 13:08:22 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{Rh4-lacZ.PD} construct and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York University *F (6/03). *F In P{Rh4-lacZ.PD}, lacZ expression is driven by the rhodopsin 4 *F promoter. This construct was constructed in the same manner as the other *F rhodopsin-lacZ transgenes described in Papatsenko et al., 2001 (Mech. Dev. *F 101:143-153; FBrf0134746). *F P{Rh4-lacZ.PD}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{Rh4-lacZ.PD}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178893 *a Luo *b H. *t 2004.5 *T personal communication to FlyBase *u P{Hsp70-Draf.gof}3 insertion. *F Date: Thu, 08 Jul 2004 13:14:58 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{Hsp70-Draf.gof}3 insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Hong Luo, Texas A&M University Health Science Center (5/04). *F P{Hsp70-Draf.gof}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178894 *a Cook *b H. *t 2004.6 *T personal communication to FlyBase *u P{UASp-armi-GFP} insertions. *F Date: Thu, 08 Jul 2004 13:44:39 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UASp-armi-GFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Heather Cook, University of Massachusetts Medical School *F (6/04). *F P{UASp-armi-EGFP}2 is a second chromosome insertion. *F P{UASp-armi-EGFP}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178895 *a Deal *b M. *c J. *d Deal *e K. *f Cook *t 2004.7.8 *T personal communication to FlyBase *u Isolation and characterization of Df(2R)BSC54. *F Date: Thu, 08 Jul 2004 13:56:22 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2R)BSC54 *F Cc: jedeal@helix.cgb.indiana.edu, medeal@bio.indiana.edu *F Isolation and characterization of Df(2R)BSC54 *F Megan Deal, Jennifer Deal and Kevin Cook *F Bloomington Stock Center, Indiana University *F Df(2R)BSC54 was isolated was a P transposase-induced male recombination *F event involving P{lacW}walk14026 and P{PZ}l(2)0024800248. The deletion *F was isolated as a cn+-bw+ recombinant chromosome from the cross cn1 *F bw1 females X cn1 P{PZ}l(2)0024800248/ P{lacW}walk14026 bw1 *F sp1; TMS, Sb1 P{Delta2-3}/+ males. Polytene chromosome squashes showed *F the breakpoints 48B6-C1;48C5-D1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178896 *a Deal *b M. *c J. *d Deal *e K. *f Cook *t 2004.7.8 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC53. *F Date: Thu, 08 Jul 2004 13:59:08 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC53 *F Cc: medeal@bio.indiana.edu, jedeal@helix.cgb.indiana.edu *F Isolation and characterization of Df(2L)BSC53 *F Megan Deal, Jennifer Deal and Kevin Cook *F Bloomington Drosophila Stock Center *F Df(2L)BSC53 was isolated as a P transposase-induced male recombination *F event involving P{lacW}Trf1 and P{PZ}lmg03424. The deletion was *F isolated as a dpov1-cn1 recombinant chromosome from the cross dpov1 *F cn1 bw1 females X dpov1 P{lacW}Trf1/P{PZ}lmg03424 cn1; TMS, *F Sb1 P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the *F breakpoints 29A2-B1;29D2-E1. Df(2L)BSC53 failed to complement *F fy2. Females heterozygous for the deletion are sterile with misshapen *F eggs. Males heterozygous for the deletion are fertile. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178897 *a Andrade *b R. *c J. *d Deal *e M. *f Deal *g K. *h Cook *t 2004.7.8 *T personal communication to FlyBase *u Isolation and characterization of Df(3R)BSC55. *F Date: Thu, 08 Jul 2004 14:03:52 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3R)BSC55 *F Cc: Rachel Andrade , jedeal@helix.cgb.indiana.edu, *F medeal@bio.indiana.edu *F Isolation and characterization of Df(3R)BSC55 *F Rachel Andrade, Jennifer Deal, Megan Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC55 was isolated as a P transposase-induced male recombination *F event involving P{hsneo}hhneo56 and P{PZ}klgrN712. The deletion was *F isolated as a st+-ca+ recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{hsneo}hhneo56/P{PZ}klgrN712 ca1 males. Polytene chromosome squashes *F showed the breakpoints 94D2-10;94E1-6. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178898 *a Parks *b A. *t 2004.7.12 *T personal communication to FlyBase *u *F Date: Mon, 12 Jul 2004 10:06:56 \-0500 *F To: flybase-updates@morgan.harvard.edu, rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-lacZ.Exel} construct and insertions *F Cc: 'Annette Parks' *F The following information was provided by Annette Parks of Exelixis, Inc. *F In P{UAS-lacZ.Exel}, lacZ was cloned into EcoR1/Bgll2 sites in the *F polylinker of P{Express-UAS}. The lacZ gene was isolated in two fragments: *F EcoR1-Sac1 was from pP{C4-AUG-Betagal} (FBmc0000220) and is an in-frame *F fusion of the ADH leader to the 5' end of lacZ. The other half of lacZ was *F from pDCZA (a clone from Dan Curtis) and has the 3' end of lacZ directly *F fused to the ADH 3'UTR immediately following the stop codon. *F P{UAS-lacZ.Exel}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-lacZ.Exel}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178899 *a Jackson *b G. *t 2004.7.21 *T personal communication to FlyBase *u P{GMR-HD.Q120} insertions. *F Date: Wed, 21 Jul 2004 16:04:23 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GMR-HD.Q120} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by George Jackson, UCLA (6/04). *F P{GMR-HD.Q120}2.4 is a homozygous viable and fertile, second chromosome *F insertion. *F P{GMR-HD.Q120}4.62 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178900 *a Kreber *b R. *t 2004.7.20 *T personal communication to FlyBase *u *F Date: Tue, 20 Jul 2004 14:53:23 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: T(2;3)SM6b-TM6B *F The following information was provided by Robert Kreber, University of *F Wisconsin at Madison (7/04). *F T(2;3)SM6b-TM6B is a gamma ray-induced translocation between SM6b *F (FBba0000040) and TM6B, Tb1 (FBba0000197) balancers. The translocation *F breakpoints are in polytene chromosome divisions 34 and 84. In addition to *F the markers usually present on the progenitor chromosomes (including Cy1, *F amosRoi-1, AntpHu and Tb1), T(2;3)SM6b-TM6B carries a strong allele *F of ap. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178901 *a Roote *b J. *t 2004.7.20 *T personal communication to FlyBase *u *F To: Rachel Drysdale (Genetics) , steve Russell , Ed Ryder , michael *F Ashburner (Genetics) , Mechler Prof. Bernard *F *F From: John Roote *F Subject: ED genetic confirmation *F Date: Tue, 20 Jul 2004 17:49:52 \+0100 *F These ED deletions have been confirmed genetically, by lack of *F complementation: *F Df gene allele *F Df(3R)ED5196 cas j1C2 *F Df(2L)ED489 wg 1 *F wg Cx1 *F Df(3R)ED5021 hkb 2 *F tub 2 *F Df(3R)ED5071 Kary&bgr;3 j3A4 *F opa 1 *F tub 2 *F Df(3R)ED5066 opa 1 *F Kary&bgr;3 j3A4 *F Df(3R)ED5147 cno 2 *F Hph 02255 *F Df(3R)ED5223 dsx 19 *F Tom34 03692 *F Df(3R)ED5177 eIF-5C 03022 *F Atu s1938 *F Df(3R)ED5138 opa 1 *F corto 07128b *F Df(3R)ED5156 Hph 02255 *F Df(3R)ED5020 hkb 2 *F tub 2 *F Df(3R)ED5100 tub 2 *F corto 07812b *F Kary&bgr;3 j3A4 *F Df(3R)ED5046 tub 2 *F hkb 2 *F Df(3R)ED5221 rn 3 *F Gld n1 *F l(3)s2214 (=CG10061)s2214 # *U FBrf0178902 *a Bloomington Drosophila Stock Center *b ?. *t 2004.7.26 *T personal communication to FlyBase *u *F Date: Mon, 26 Jul 2004 19:26:14 \-0500 (EST) *F From: Kathy Matthews *F Subject: In(2LR)Gla *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: In(2LR)Gla *F Gla is a commonly used synonym for In(2LR)Gla. Please add this *F synonym to the record for FBab0004765. # *U FBrf0178903 *a Gramates *b S. *t 2004.7.26 *T personal communication to FlyBase *u GUKholder annotation update. *F From: siang@mcb.umass.edu *F Date: Mon, 26 Jul 2004 09:58:14 \-0400 *F Subject: GUKholder annotation update (attn: Kathy) 1 of 2 *F To: flybase-updates@morgan.harvard.edu *F Dear Kathy, *F Here is some information (perhaps too much information) concerning GUKholder, *F including discrepancies between the sequence I have proposed and the sequence *F in FlyBase. The big discrepancy is in the fifth exon; I strongly believe that *F the actual fifth exon is much shorter than that currently in the database; I've *F included a bit of text from my dissertation as to how I arrived at my proposed *F fifth exon (the splice site I chose in the fifth exon is the only potential *F splice site that puts the remainder of the open reading frame in-frame with the *F fourth exon, and gives a predicted protein with a molecular weight that comes *F anywhere close to corresponding to the size predicted by Western analysis). *F Please let me know if anything needs clarification. *F Cheers, *F Sian Gramates *F 'Genomic analysis *F The gukholder (gukh) gene was mapped to position 91E-F of the right arm of the *F third chromosome by in situ hybridization (Langer-Safer, 1982). This position *F was refined to 91E2-3 by exploitation of the recently completed sequencing of *F the Drosophila genome (Adams, et al., 2000). The sequence of the EST GM02806 *F was used in a BLAST (Altschul, et al., 1990; Gish and States, 1993) search of *F the Drosophila genome. This predicted that the gukh gene is comprised of 5 *F exons spread over a 38 kB region). The 3' splice boundary of the fourth exon *F and the 5' splice boundary of the fifth exon result in a frame-shift, *F suggesting the possibility of an intervening sixth exon (Kudo, et al., 1987; *F Vignal, et al., 1996; Rogozin and Milanesi, 1997). The first 140 bases of the *F EST GM06978 include a 3' splice boundary that is in frame with final gukh exon; *F however the 5' end of GM06978 is not a predicted splice acceptor. Analysis of *F the genomic region upstream of the predicted fifth exon reveals a splice *F acceptor site 321 bases upstream of the genomic site corresponding to the 5' *F end of GM06978, which is in-frame with the 3' splice boundary of the fourth *F exon predicted by the sequence of GM02806. The six exons predict a 3132 bp *F open reading frame predicting a 1044 amino acid protein with a predicted *F molecular weight of 111.4 kDa, corresponding almost exactly to the molecular *F weight observed by Western blot analysis (see below). *F BDGP has subjected the completed Drosophila sequence to analysis by the gene *F prediction program Genie (Kulp, et al., 1996; Reese, et al., 1997), to predict *F sequences potentially corresponding to genes, and deposited the results in the *F GadFly database (Reese, et al., 2000). GadFly predicts three novel genes of *F unknown function, CG5456, CG14288 and CG6003 in region covered by gukh. These *F three predicted genes appear to in fact be a single gene, gukholder, with the *F first exon of CG5456 and the second exon of CG14288 corresponding to the first *F and second exons of gukh, and CG6003 accounting for the remaining three exons. *F Interestingly, there are no known or predicted genes lying in the 23.4 kB *F intron between the first two gukh exons. GM02806 does not include he smaller *F exons of predicted genes CG5456 or CG14288, nor does it include the large *F penultimate exon of predicted gene CG6003, although the predicted fifth gukh *F exon overlaps the final 461 bp of that exon. However, the 3' end of dEST *F AT22588 (Genbank accession number BF487615), which includes the first four *F exons of gukh, overlaps the first 1893 bp of that 2.7 kB CG6003 exon before *F ending in a poly-A tail.' *F This is the protein sequence I've predicted for GUKH, with some sequence *F analysis: *F MPFVQRVVQPVNVAQATAASLGHASGRFHCTPGKCRNNNCINKQSPDVEV 50 *F PNGHGSPTSLTRVLVHQAPEDQPVPPAPAPMKKLKHHVEFLSTSPTRHQS 100 *F QSLPNSRHPSQQRPAIGRIASAPQSPTAGSSKVVSGHEFDSISNITLSNA 150 *F LRQLASLVLIGSDIFDDLQRDLQAVGERAGRVQRKIAAVERRVCAYDPKT 200 *F VTVPESDLLTFAQRKHHFETDKSFQQELFTGETRPLSVRQLYTEAGKELP 250 *F VATGATATALASMAHSQSLIPSRSISFNGEVLSSDHEVLQLNGSTDTEDH 300 *F LLCVSDFGNANRKLRTRIDAEIEIRLPAAIEDLRKWTSSEALGDVTVTPD 350 *F CMHHVDTSVSTSLAIGENGILTPALSPSVLSADAVDALYAQNLSQAADSS 400 *F RHNHHTQALIPNDINKDVPLNHRLPSPEEQTKQIALKYPAEVISVNTSGK 450 *F HFQRMCAARKSTSGGYAGGATAGSSSSTSPENAGQTEGNNLDDNVQTVSR 500 *F RSRSRKVRGKRRNTIAGIDQKEIQDAANGLKRRKGAHTTFAPGPYVKETE 550 *F SALHSAESAAAAIASSQDPSAASGAISSTKSAKLLLLTSGGLISGNRKVA 600 *F NVRPIVAKSPLSRNVSEMATYQATSYQDQKQLLANRTNPFYETIAAPVAN 650 *F LMMPSPSGKSNSSTGSTATSSSSSASGQSGEEGYQRFSNIYEQVQPAPST 700 *F QPQEDATATPFQRSAPIYWTLQNRRSQPRPQPAGNVTCRDDLYATPIRRA 750 *F DRLPRPVAAAAATAAEGNRSNISPIVPRNRSGAFLTSTPTRSGEGERDSE 800 *F NTQLPPMKQPSRNWTDDAPERLNTCADRIGQSKTTLMDFKKLLLAKSAKA 850 *F SPVQRKVSAVELLKKPAQATATPPVVKPSGPGQKVASAGSKPTINTSLKL 900 *F LDLSGSPKTFANRRMLRQGQFGSPSKTFAPKMRGPVNMVAAAAAPPRTDI 950 *F MSTTIPEANSEEDHSNTSGGSRASSEAGETVPSSFDLKRNFFLQTEENNF 1000 *F MRGELKPSFARSNGAAGGGSTEGVNKYVAAPVANPPLPSFETAL 1044 *F WH1-like domain: aa 130-251 *F regions with homology to Kelch long form: aa 603-803, 852-977, 982-1043 *F GUKholding domain: aa 889-1044 *F PDZ binding motif: aa 1041-1044 *F PEST region: aa 947-963 *F Here's the predicted coding sequence: *F ATGCCCTTCGTCCAGCGCGTGGTGCAGCCCGTCAATGTGGCCCAGGCCACGGCGGCCAGTTTGGGCCACGCATCCGGAC *F GATTCCACTGCACGCCGGGCAAGTGCCGGAACAACAACTGCATTAACAAGCAGTCGCCGGATGTAGAGGTCCCCAATGG *F CCACGGATCACCCACATCCCTTACCAGGGTTCTGGTTCACCAGGCGCCGGAGGATCAACCCGTACCGCCCGCTCCGGCG *F CCCATGAAGAAGCTAAAGCACCACGTCGAGTTCCTGTCCACCTCGCCCACTCGCCATCAGTCGCAATCCCTGCCCAATT *F CGCGGCATCCCAGCCAGCAGAGGCCGGCGATTGGAAGGATCGCCAGTGCACCGCAGTCGCCCACTGCCGGCTCCTCCAA *F GGTGGTCTCCGGCCACGAGTTCGACTCGATCAGCAACATAACGCTGAGCAACGCCCTGCGGCAGCTGGCCAGCTTGGTC *F CTCATAGGCAGCGATATCTTCGACGACCTGCAGCGGGATCTGCAGGCGGTGGGCGAGCGGGCGGGTCGCGTGCAGCGGA *F AGATTGCCGCCGTGGAGCGACGCGTCTGTGCCTACGATCCGAAAACGGTCACAGTTCCTGAAAGCGACCTGCTCACCTT *F CGCACAGCGCAAACACCACTTCGAGACGGACAAGTCATTCCAGCAGGAGCTCTTTACAGGCGAGACCAGACCTCTGAGC *F GTCCGGCAGCTTTACACGGAGGCTGGTAAGGAGCTGCCGGTGGCCACCGGAGCCACTGCGACTGCCCTCGCCTCCATGG *F CGCACTCGCAGTCGCTGATCCCGTCCCGTTCCATCTCCTTCAACGGTGAGGTTCTGTCCAGCGATCACGAAGTTCTACA *F ACTAAATGGATCCACGGACACGGAGGATCATCTGCTGTGCGTGTCGGACTTTGGCAATGCCAACCGCAAGCTGCGCACG *F CGCATCGATGCCGAGATCGAGATCCGACTGCCCGCTGCCATTGAGGATTTGCGGAAGTGGACGTCCAGCGAGGCGCTGG *F GTGATGTCACCGTCACGCCGGATTGCATGCATCACGTGGACACCTCGGTGAGCACCTCGCTGGCCATCGGCGAGAATGG *F CATCCTGACCCCGGCGCTGTCACCGTCCGTCCTATCCGCCGATGCCGTCGATGCCCTCTACGCCCAGAATCTGAGCCAG *F GCGGCGGACAGCTCCCGGCACAACCACCACACGCAAGCCCTCATCCCGAACGATATTAATAAAGACGTGCCTTTGAATC *F ATCGATTGCCTTCACCCGAAGAACAGACCAAGCAGATTGCCTTAAAATACCCCGCCGAAGTGATTTCGGTGAACACCAG *F CGGTAAGCACTTCCAGCGGATGTGTGCGGCTCGCAAGTCGACCAGTGGAGGATACGCAGGTGGCGCCACCGCCGGATCC *F TCGAGCAGCACATCGCCGGAGAACGCCGGCCAAACGGAGGGCAACAACCTGGACGACAATGTGCAGACGGTGAGCCGGA *F GGTCAAGATCGCGCAAGGTGCGCGGAAAGCGGCGGAACACGATAGCCGGAATCGATCAGAAGGAGATCCAGGATGCGGC *F TAATGGTCTGAAGCGGCGCAAGGGTGCACACACCACCTTCGCACCTGGGCCGTACGTGAAGGAAACCGAAAGTGCGCTC *F CACAGTGCTGAAAGTGCAGCGGCGGCAATAGCATCGTCACAGGATCCATCAGCAGCATCAGGAGCCATTAGCAGTACCA *F AGAGCGCCAAGCTCCTACTGCTCACCAGCGGCGGATTAATCTCTGGCAATCGCAAGGTGGCCAACGTGAGGCCCATTGT *F GGCCAAATCCCCGCTCAGCAGGAACGTCAGCGAAATGGCAACCTACCAGGCAACCAGCTACCAGGACCAGAAACAACTG *F CTGGCCAACCGAACAAATCCCTTCTACGAGACCATTGCCGCACCCGTGGCCAATCTGATGATGCCCTCGCCGTCGGGCA *F AGTCCAATTCTTCGACGGGCTCCACAGCCACCAGTTCCTCCTCATCGGCATCGGGTCAATCCGGTGAAGAAGGGTATCA *F ACGTTTCTCTAATATCTATGAGCAAGTGCAGCCAGCACCGAGCACCCAGCCACAGGAAGATGCGACTGCCACGCCCTTC *F CAGCGCAGTGCGCCCATCTATTGGACACTGCAGAATCGCCGCAGCCAGCCAAGGCCACAGCCCGCTGGCAACGTGACCT *F GCCGGGATGACCTCTACGCCACGCCCATTCGACGGGCCGACCGACTGCCACGCCCAGTGGCTGCTGCTGCGGCAACAGC *F AGCGGAAGGCAACAGGAGCAACATATCGCCAATTGTGCCAAGGAATCGTTCGGGAGCCTTCCTCACCTCCACGCCCACG *F AGGAGCGGAGAGGGTGAACGCGATTCGGAGAACACACAACTTCCCCCCATGAAGCAGCCATCCCGCAACTGGACCGATG *F ACGCACCCGAACGCCTCAACACCTGTGCGGATCGCATTGGCCAAAGCAAGACCACGCTAATGGACTTTAAGAAGCTGCT *F GCTGGCCAAGTCCGCCAAGGCGAGTCCGGTCCAAAGGAAGGTATCCGCCGTGGAGCTGCTGAAGAAGCCAGCCCAAGCC *F ACCGCAACGCCGCCCGTTGTAAAGCCCAGTGGCCCTGGACAGAAGGTAGCATCAGCTGGAAGCAAACCAACGATCAACA *F CCAGCCTAAAGCTGCTAGATCTGAGCGGCTCACCCAAGACTTTCGCCAACCGGAGGATGCTTCGGCAAGGCCAGTTTGG *F ATCGCCCTCCAAGACCTTTGCTCCAAAGATGCGAGGACCCGTGAATATGGTGGCTGCTGCAGCGGCTCCACCGCGAACT *F GACATCATGTCCACCACGATTCCGGAGGCGAATAGCGAGGAGGATCACTCCAACACCAGCGGAGGATCGCGAGCCAGTT *F CCGAGGCGGGCGAAACGGTGCCCTCCAGCTTCGACTTGAAGCGGAACTTCTTTTTGCAAACCGAGGAGAACAATTTCAT *F GCGCGGGGAGCTGAAGCCCAGCTTTGCCAGGTCAAATGGAGCAGCCGGAGGTGGCAGCACCGAAGGCGTCAACAAGTAC *F GTGGCAGCTCCGGTGGCGAATCCTCCGCTGCCCTCGTTTGAAACGGCGTTGTAG *F Here's my analysis of the genomic region. This lies within section 49 of the *F genomic scaffold, circa 1991. Adding 14,716,602 will align the numbers below *F with the current scaffold. Each element has three rows of numbers. The first *F row is where in the predicted gene or EST the sequence lies. The second row is *F where that sequence lies in the genomic scaffold. The third row is the size of *F the exons (in parentheses) or introns in brackets. I will attach this as a *F file to a separate message, as I fear some crucial formatting may be lost. I *F will also attach an alignment of the GUKH protein sequence I predict with the *F GUKH protein sequence currently in Flybase. *F (FlyBase curator comment: filename Gramates.2004.7.26-1.rtf). *F CG5456 *F 1 \- 1069 1070 \- 1275 *F 121972 \- 123040 125285 \- 125490 *F (1069) 2245 (205) *F CG14288 *F 1 \- 37 38 \- 409 405 \- 438 *F 145764 \- 145800 146426 \- 146800 146886 \- 146919 *F (37) 626 (371) 86 (35) *F CG6003 *F 1 \- 371 372 \- 647 648 \- 3342 3343 \- 4429 *F 149501 \- 149871 151153 \- 151428 153770 \- 156464 158909 \- 159995 *F (371) 1282 (224) 2342 (2694) 2445 (1086) *F GUKH *F 1 \- 610 611 \- 975 976 \- 1307 1308 \- 1586 *F 1587 \- 2047 *F aa 1 \- 204 204 \- 325 326 \- 436 436 \- 523 *F 523 \- 683 *F 122431 \- 123040 146429 \- 146796 149540 \- 149871 151153 \- 151428 *F 156002 \- 156462 *F (610) 23389 (368) 2744 (332) 1282 (276) *F 4574 (461) 2446 *F 2048 \- 3135 *F 683 \- 1044 *F 158908 \- 159995 *F (1086) *F GM02806 GM02806 (Genbank accession number AA695424 *F 1 \- 1039 1040 \- 1407 1408 \- 1739 1740 \- 2018 *F 2019 \- 3500 2672 *F \-433 \- 610 611 \- 975 976 \- 1307 1308 \- *F 1586 *F 2048 \- 3135 *F 122001 \- 123040 146429 \- 146796 149540 \- 149871 151153 \- 151428 *F 158908 \- 160390 159995 *F (1039) 23389 (368) 2744 (332) 1282 (276) *F 7480 (1484) stop *F AT22588 Genbank accession number BF487615 *F 1 \- 1063 1064 \- 1431 1432 \- 1763 1764 \- 2038 *F 2039 \- 3931 *F \-453 \- 610 611 \- 975 976 \- 1307 1308 \- 1586 *F (alternative exon) *F 121977 \- 123040 146429 \- 146796 149540 \- 149871 151153 \- 151427 *F 153769 \- 155602 *F (1063) 23389 (368) 2744 (332) 1282 (276) *F 2342 (1893) *F GM06978 Genbank accession number AW941281 *F 1 \- 130 132 \- 1614 1220 *F 1918 \- 2047 2048 \- 3135 *F 156332 \- 156462 158908 \- 160390 159995 *F (129) 2446 (1484) stop *F File deposited: Gramates.2004.7.26-1.rtf ; File date: 2004.7.26 ; File size: *F 7593 ; File format: rtf # *U FBrf0178904 *a Posakony *b J. *t 2004.6 *T personal communication to FlyBase *u P{UAS-DsRed.T4.NLS} insertions. *F Date: Thu, 05 Aug 2004 17:26:06 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-DsRed.T4.NLS} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jim Posakony, University of California at San Diego (6/04). *F P{UAS-RedStinger}3 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{UAS-RedStinger}4 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-RedStinger}6 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178905 *a Parks *b A. *t 2004.6 *T personal communication to FlyBase *u P{UAS-Pten.dsRNA.Exel} construct and insertions. *F Date: Thu, 05 Aug 2004 17:07:51 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Pten.dsRNA.Exel} construct and insertions *F Cc: 'Annette Parks':; *F The following information was provided by Annette Parks of Exelixis, Inc *F (6/04). *F P{UAS-Pten.dsRNA.Exel} is a transgene construct which generates dsRNA *F transcripts for RNA interference of the Pten gene. Nucleotides 295 through *F 819 from Pten sequence AF201905 were cloned in the forward and reverse *F directions into pJM1084 (the hairpin vector described in Reichhart et al. *F 2002, genesis 34:160-164; FBrf0152370). The cloned sequences differed from *F AF201905 by an A to C change at nucleotide 423. *F P{UAS-Pten.dsRNA.Exel}1 is a homozygous and hemizygous viable and fertile, *F X chromosome insertion. *F P{UAS-Pten.dsRNA.Exel}2 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-Pten.dsRNA.Exel}3 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0178906 *a Reichhart *b J.M. *t 2004.8 *T personal communication to FlyBase *u pJM1084. *F Date: Thu, 05 Aug 2004 17:06:34 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: pJM1084 *F Cc: 'Annette Parks':; *F The following information was provided by Jean-Marc Reichhart, CNRS, *F Institut de Biologie Moléculaire et Cellulaire, Strasbourg (8/04). *F pJM1084 is the name used by Reichhart and colleagues for the vector *F described in Reichhart et al. 2002, 'Splice-activated UAS hairpin vector *F gives complete RNAi knockout of single or double target transcripts in *F Drosophila melanogaster', genesis 34:160-164 (FBrf0152370), which carries *F the intron of the mub gene and was used for generating most of the *F UAS-dsRNA transgenic constructs in the paper. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179686 *a Richards *b S. *c Y. *d Liu *e B.R. *f Bettencourt *g P. *h Hradecky *i S. *j Letovsky *k R. *l Nielsen *m K. *n Thornton *o M.J. *p Hubisz *q R. *r Chen *q R.P. *r Meisel *q O. *r Couronne *q S. *r Hua *q M.A. *r Smith *q P. *r Zhang *q J. *r Liu *q H.F. *r Bussemaker *q M.F. *r van Batenburg *q S.L. *r Howells *q S.E. *r Scherer *q E. *r Sodergren *q B.B. *r Matthews *q M.A. *r Crosby *q A.J. *r Schroeder *q D. *r Ortiz-Barrientos *q C.M. *r Rives *q M.L. *r Metzker *q D.M. *r Muzny *q G. *r Scott *q D. *r Steffen *q D.A. *r Wheeler *q K.C. *r Worley *q P. *r Havlak *q K.J. *r Durbin *q A. *r Egan *q R. *r Gill *q J. *r Hume *q M.B. *r Morgan *q G. *r Miner *q C. *r Hamilton *q Y. *r Huang *q L. *r Waldron *q D. *r Verduzco *q K.P. *r Clerc-Blankenburg *q I. *r Dubchak *q M.A.F. *r Noor *q W. *r Anderson *q K.P. *r White *q A.G. *r Clark *q S.W. *r Schaeffer *q W. *r Gelbart *q G.M. *r Weinstock *q R.A. *r Gibbs *t 2004.11.19 *T personal communication to FlyBase *u .; Verduzco,D.; Clerc-Blankenburg,K.P.; Dubchak,I.; Noor,M.A.F.; Anderson,W.; White,K.P.; Clark,A.G.; Schaeffer,S.W.; Gelbart,W.; Weinstock,G.M.; Gibbs,R.A. *F CM000070 2 E 2 *F CM000071 3 C 3 *F CH379058 4_group1 B 4 *F CH379059 4_group2 B 4 *F CH379060 4_group3 B 4 *F CH379061 4_group4 B 4 *F CH379062 4_group5 B 4 *F CH379081 Unknown_group10 Contig1154_Contig1157 A XL *F CH379175 Unknown_group104 Contig2487_Contig3123 E 2 *F CH379181 Unknown_group110 Contig2630_Contig4588 A XL *F CH379183 Unknown_group112 Contig2662_Contig3063 A XL *F CH379189 Unknown_group118 Contig2792_Contig7768 A XL *F CH379190 Unknown_group119 Contig2797_Contig5818 C 3 *F CH379191 Unknown_group120 Contig2812_Contig5955 C 3 *F CH379196 Unknown_group125 Contig289_Contig290 A XL *F CH379198 Unknown_group127 Contig2917_Contig2974 C 3 *F CH379204 Unknown_group133 Contig3010_Contig7301 B 4 *F CH379205 Unknown_group134 Contig3018_Contig3021 E 2 *F CH379208 Unknown_group137 Contig3064_Contig5137 F 5 *F CH379213 Unknown_group142 Contig3173_Contig7882 C 3 *F CH379217 Unknown_group146 Contig3286_Contig7811B E 2 *F CH379219 Unknown_group148 Contig332_Contig6133 F 5 *F CH379225 Unknown_group154 Contig3400_Contig702 A XL *F CH379234 Unknown_group163 Contig3628_Contig4336 A XL *F CH379241 Unknown_group170 Contig3721_Contig4526 A XL *F CH379250 Unknown_group179 Contig3774_Contig4907 E 2 *F CH379089 Unknown_group18 Contig1248_Contig1249 B 4 *F CH379258 Unknown_group187 Contig3999_Contig6922 A XL *F CH379090 Unknown_group19 Contig124_Contig7227 F 5 *F CH379262 Unknown_group191 Contig4007_Contig5983 A XL *F CH379264 Unknown_group193 Contig4021_Contig4574 A XL *F CH379091 Unknown_group20 Contig1260_Contig5691 C 3 *F CH379271 Unknown_group200 Contig4081_Contig4441 B 4 *F CH379279 Unknown_group208 Contig4243_Contig6441 C 3 *F CH379280 Unknown_group209 Contig4287_Contig2616 D XR *F CH379282 Unknown_group211 Contig4295_Contig5750 A XL *F CH379284 Unknown_group213 Contig429_Contig7537 A XL *F CH379288 Unknown_group217 Contig4337_Contig685 A XL *F CH379295 Unknown_group224 Contig4383_Contig4487 A XL *F CH379296 Unknown_group225 Contig4386_Contig6496 F 5 *F CH379302 Unknown_group231 Contig4444_Contig6445 F 5 *F CH379303 Unknown_group232 Contig4447_Contig7777 B 4 *F CH379306 Unknown_group235 Contig4474_Contig4463 F 5 *F CH379308 Unknown_group237 Contig4502_Contig7037 F 5 *F CH379311 Unknown_group240 Contig4523_Contig4837 F 5 *F CH379318 Unknown_group247 Contig4644_Contig479 F 5 *F CH379327 Unknown_group256 Contig4715_Contig6685 A XL *F CH379097 Unknown_group26 Contig1353_Contig6925 C 3 *F CH379336 Unknown_group265 Contig4802_Contig8008 E 2 *F CH379338 Unknown_group267 Contig4861_Contig5457 F 5 *F CH379344 Unknown_group273 Contig4930_Contig5467 B 4 *F CH379350 Unknown_group279 Contig4971_Contig7717B D XR *F CH379353 Unknown_group282 Contig4988_Contig6063 A XL *F CH379355 Unknown_group284 Contig5002_Contig6227 B 4 *F CH379366 Unknown_group295 Contig5106_Contig6084 B 4 *F CH379370 Unknown_group299 Contig5146_Contig7791 B 4 *F CH379375 Unknown_group304 Contig5222_Contig6173 F 5 *F CH379105 Unknown_group34 Contig1480_Contig4618 A XL *F CH379411 Unknown_group340 Contig5519_Contig6347 A XL *F CH379414 Unknown_group343 Contig5547_Contig5814 B 4 *F CH379417 Unknown_group346 Contig5605_Contig6211 F 5 *F CH379420 Unknown_group349 Contig5651_Contig7045 A XL *F CH379426 Unknown_group355 Contig5746_Contig5758 C 3 *F CH379429 Unknown_group358 Contig5777_Contig5904 C 3 *F CH379432 Unknown_group361 Contig580_Contig5863 A XL *F CH379434 Unknown_group363 Contig5819_Contig6648 A XL *F CH379438 Unknown_group367 Contig5876_Contig6338 A XL *F CH379443 Unknown_group372 Contig5960_Contig5609 F 5 *F CH379446 Unknown_group375 Contig5987_Contig2564 C 3 *F CH379460 Unknown_group389 Contig6198_Contig2476 C 3 *F CH379465 Unknown_group394 Contig6260_Contig6412 F 5 *F CH379467 Unknown_group396 Contig6264_Contig6779 A XL *F CH379469 Unknown_group398 Contig6285_Contig7427 C 3 *F CH379483 Unknown_group412 Contig6461_Contig5822 A XL *F CH379485 Unknown_group414 Contig6483_Contig5354 A XL *F CH379491 Unknown_group420 Contig6579_Contig6580 D XR *F CH379493 Unknown_group422 Contig6617_Contig2972 E 2 *F CH379494 Unknown_group423 Contig6631_Contig6935 B 4 *F CH379500 Unknown_group429 Contig6701_Contig7372 F 5 *F CH379501 Unknown_group430 Contig6722_Contig7220 F 5 *F CH379503 Unknown_group432 Contig673_Contig3619 F 5 *F CH379504 Unknown_group433 Contig6754_Contig6493 D XR *F CH379507 Unknown_group436 Contig6833_Contig634 F 5 *F CH379509 Unknown_group438 Contig6887_Contig6862 F 5 *F CH379521 Unknown_group450 Contig7064_Contig7123 A XL *F CH379524 Unknown_group453 Contig7149_Contig5860 A XL *F CH379528 Unknown_group457 Contig721_Contig722 A XL *F CH379546 Unknown_group475 Contig751_Contig752 D XR *F CH379548 Unknown_group477 Contig7528_Contig7035 C 3 *F CH379553 Unknown_group482 Contig7582_Contig6713 C 3 *F CH379556 Unknown_group485 Contig7635_Contig6082 D XR *F CH379560 Unknown_group489 Contig7760_Contig5448 F 5 *F CH379561 Unknown_group490 Contig7818_Contig6770 F 5 *F CH379567 Unknown_group496 Contig7914_Contig3103 A XL *F CH379569 Unknown_group498 Contig7926_Contig2810 B 4 *F CH379076 Unknown_group5 Contig1100_Contig5371 A XL *F CH379571 Unknown_group500 Contig8094_Contig5509 F 5 *F CH379573 Unknown_group502 Contig8196_Contig4747 A XL *F CH379125 Unknown_group54 Contig1849_Contig319 B 4 *F CH379126 Unknown_group55 Contig1861_Contig7944 B 4 *F CH379148 Unknown_group77 Contig2087_Contig2088 F 5 *F CH379149 Unknown_group78 Contig2114_Contig927 B 4 *F CH379152 Unknown_group81 Contig2131_Contig6302 A XL *F CH379154 Unknown_group83 Contig2182_Contig7948 A XL *F CH379156 Unknown_group85 Contig2258_Contig2260 A XL *F CH379159 Unknown_group88 Contig229_Contig230 B 4 *F CH379161 Unknown_group90 Contig2325_Contig3380 B 4 *F CH379164 Unknown_group93 Contig2352_Contig8140 A XL *F CH379165 Unknown_group94 Contig2353_Contig2355 A XL *F CH379167 Unknown_group96 Contig2399_Contig5935 A XL *F CH379170 Unknown_group99 Contig2430_Contig4320 E 2 *F CH379063 XL_group1a A XL *F CH379064 XL_group1e A XL *F CH379065 XL_group3a A XL *F CH379066 XL_group3b A XL *F CH379067 XR_group3a D XR *F CH379068 XR_group5 D XR *F CH379069 XR_group6 D XR *F CH379070 XR_group8 D XR *F CH379071 XR_group9 D XR # *U FBrf0179751 *a Goode *b S. *t 2004 *T personal communication to FlyBase *u *F Date: Tue, 7 Dec 2004 11:16:25 \-0600 *F To: David Sutherland *F From: Scott Goode *F Subject: Re: FlyBase Construct Query *F David, *F I believe it is an enhancer trap. It maps to the third chromosome, and is *F apparently a semi-lethal line since it is balanced with TM3, Ser, and *F occasionally *F we see homozygous (Ser+) flies. *F Hope that helps. *F Scott *F >Dear Scott, *F > *F >I'm trying to track down details regarding a GAL4 line called BA3, mentioned *F >your Development paper 'Szafranski and Goode, 2004, Development 131(9): *F >2023--2036', and credited to Trudi Schupbach. Do you have any more *F >details you *F >could send me on this driver?. Is it an enhancer trap or a *F >construct? If it is *F >the former, do you know what insertion construct was used &/or any details of *F >where it maps. If the latter, do you have any details of its *F >construction. Any *F >details you can send would be much appreciated. *F > *F >Many thanks, *F > *F >David Sutherland *F > *F >FlyBase, *F >Cambridge UK # *U FBrf0179752 *a Dura *b J.M. *t 2004.4.7 *T personal communication to FlyBase *u *F Date: Wed, 07 Apr 2004 11:44:12 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{C4-812}10-2 and P{C4-813}4-37 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jean-Maurice Dura, Institut de Genetique Humaine, CNRS *F Montpellier. *F P{C4-812}10-2 and P{C4-813}4-37 are homozygous viable and fertile, *F autosomal insertions. The construction and transformation of P{C4-812} and *F P{C4-813} were described in Bloyer et al., 2003, 'Identification and *F characterization of polyhomeotic PREs and TREs', Dev. Biol. 261:426--442 *F (FBrf0167457). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179760 *a Marygold *b S. *t 2004.9.8 *T personal communication to FlyBase *u Ribosomal protein nomenclature in D.melanogaster. *F Personal communication from: S. Marygold *F Subject: Ribosomal protein nomenclature in D.melanogaster. *F Date: Wed, 8 Sep 2004 *F 1. Our RP expert Naoya Kenmochi confirmed to me that 'Human MRPL42 and *F MRPS32 are the same. I suggest that you take mRpL42 for this protein *F to avoid any confusion'. If we can't do what he suggests (because this *F gene is already known as 'mRpS32' in FB), can we add 'mRpL42' as a *F synonym of mRpS32? *F 2. Naoya also confirmed what I thought about mRpS28 and mRpS35 being the *F wrong way round: 'CG5497 corresponds to human MRPS28 and CG2101 corresponds *F to MRPS35.' So can we switch these names (ie. CG5497 = mRpS28; *F CG2101=mRpS35)? *F 3. Naoya also had this to say about mRpS4: 'MRPS4 (C15orf12) was withdrawn *F from the human MRP list. See *F http://www.gene.ucl.ac.uk/nomenclature/genefamily/MRPs.html'. So I don't *F think we should rename CG4866 as 'mRpS4'. *F 4. According to the weblink in point 3, we should rename 'mRpL7-L12' as *F just mRpL12 so as to be consistent with the current naming convention. *F 5. RpL41 and CR30425 reports. These need to be merged and if there's going *F to be a CG---- replacement for the 'CR30425', what will this be (just *F CG30425?)? *F 6. mRpS18 genes. These fly genes (and their human counterparts) are *F sufficiently divergent to warrant these genes being renamed as *F follows: *F mRpS18a (CG9688) should be renamed mRpS18C *F mRpS18b (CG10757) should be renamed mRpS18B *F mRpS18c (CG31450) should be renamed mRpS18A *F That is, these three genes are not really 'paralogs' in the same sense *F as RpL34a and RpL34b, for example. Rather, they are distinct genes *F with only limited similarity between them, and the current FB names *F don't really tally with their 'official' names of their human *F orthologs. *F 7. CG33002 should be renamed mRpL27 (this is the only MRPL27 ortholog). *F 8. CG18767 should be renamed mRpL36 (this is the only MRpL36 ortholog). *F 9. mRpL2a (CG6547) should be renamed mRpL37 (this is the only MRpL37 *F ortholog. There is no official 'MRPL2a' classification). *F 10. mRpL3a (CG15871) should be renamed mRpL38 (this is the only MRPL38 *F ortholog. There is no official 'MRPL3a' classification). *F 11. mRpL5 (CG17166) should be renamed mRpL39 (this is the only MRPL39 *F ortholog. There is no official 'MRPL5' classification). *F 12. mRpL22-24 (CG5242) should be renamed mRpL40 (this is the only MRPL40 *F ortholog. There is no official 'MRPL22-24' classification). *F 13. CG12954 should be renamed mRpL41 (this is the only MRPL41 ortholog). *F 14. CG30481 should be renamed mRpL53 (this is the only MRPL53 ortholog). *F Steven Marygold Ph.D. *F Growth Regulation Laboratory (Rm 503), *F Cancer Research UK London Research Institute, *F 44 Lincoln's Inn Fields, *F LONDON, WC2A 3PX *F U.K. *F Tel: \+44 (0)20 7269 3351 *F Fax: \+44 (0)20 7269 3581 # *U FBrf0179762 *a Poole *b S. *t 2004.9.20 *T personal communication to FlyBase *u FlyBase error report for CG17835 on Mon Sep 20 20:22:08 2004. *F Date: Mon, 20 Sep 2004 20:22:08 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: poole@lifesci.ucsb.edu *F Subject: FlyBase error report for CG17835 on Mon Sep 20 20:22:08 2004 *F Error report from Stephen Poole (poole@lifesci.ucsb.edu) *F Gene or accession: CG17835 *F Gene annotation error *F Gene CG17835 has incorrect exon/intron structure. *F Comments: The annotation of all transcripts from invected (CG17835) in the *F genome database have an incorrect splice donor site at position 11968 (it *F should be 11964), and are missing a small 6 nt exon (GTCGAA) at position *F 39624..39629 (coordinates are from scaffold AE003825; gi:21627432). Loss of *F this small exon and use of the wrong splice donor results in transcripts that *F shift the reading frame and would encode a 566 nt protein that does not have *F an engrailed-like homeo domain. *F Invected and engrailed are adjacent to each other at 48A, and have *F considerable genetic redundancy. The originally reported invected cDNA clone *F Coleman, et. al. (1987) Genes and Development 1: 19-28 was predicted to *F encode a 576 aa long protein that is almost identical with engrailed in the *F region of the homeo domain, consistent with the two proteins having redundant *F functions. The sequence of the DGC cDNA clone AT24588 would encode the *F identical protein. *F The annotation likely missed the exon due to its small size (6 nt). I believe *F my annotation of this small exon is correct because: *F 1). the Coleman et al cDNA clone and the DGC clone both have this 6 nt exon. *F 2). in scaffold AE003825, position 39624 is the only instance of the sequence *F GCGGAA flanked by the invariant ag and gt splice acceptor and donor *F dinucleotides *F 3). the splice acceptor is a canonical splice acceptor: *F ttct tttctctttg cagGTCGAAg taagtacaaa *F At present, the annotation of invected is rather muddy. The predicted proteins *F for any of the 4 annotated inv transcripts would encode UniProt Q9V600, *F lacking a homeo domain, and clearly incorrect. Setting the splice donor to *F match the splice site from Coleman et al and adding the small 6 nt exon would *F make the annotation consistent with the sequence of the Coleman et al sequence *F and the DGC AT24588, and would once again make inv a 576 aa long homeobox *F protein. In addition, UniProt Q9V600 should be removed from the protein *F database. *F Steve Poole *F Dept. of MCD Biology *F UCSB *F poole@lifesci.ucsb.edu # *U FBrf0179791 *a Bloomington Drosophila Stock Center *b ?. *t 2004.11.7 *T personal communication to FlyBase *u Garza insertion alleles that are not in FlyBase. *F Date: Sun, 7 Nov 2004 15:13:31 \-0500 (EST) *F From: Kathy Matthews *F Subject: Garza insertion alleles that are not in FlyBase *F To: flybase-updates@morgan.harvard.edu *F Personal Communication from: Bloomington Drosophila Stock Center *F Subject: Garza insertion alleles that are not in FlyBase *F Dated: 7 Nov 2004 *F The 69 insertions listed below were donated to the Bloomington Stock Center by *F Brian Ring and Dan Garza (Florida State University, Novartis; see *F FBrf0152434). In collaboration with the Gene Disruption Project (GDP: H.J. *F Bellen, Baylor College of Medicine, A. Spradling, Carnegie Institute, and R. *F Hoskins, Lawrence Berkeley National Laboratory), selected lines were balanced *F or homozygosed (Ring and Garza) and resequenced (GDP). According to the *F GDP, the insertions listed below are within transcription units and create the *F indicated alleles. To avoid further asynchrony between the Stock Center and *F FlyBase, we are passing this information, which was produced by the GDP, along *F for curation. GDP's full data set on the Ring and Garza insertions will be *F submitted to FlyBase at some time in the future. *F FlyBase ID Insertion Symbol *F FBti0037879 PBac{3HPy+}Sep2C207 *F FBti0037926 PBac{3HPy+}alphaTub84DC368 *F FBti0037915 PBac{3HPy+}Arf102FC339 *F FBti0037825 PBac{3HPy+}Arf79FC005 *F FBti0037905 PBac{3HPy+}CG15282C298 *F FBti0037886 PBac{3HPy+}CG6355C227 *F FBti0037866 PBac{3HPy+}cnoC156 *F FBti0037855 PBac{3HPy+}Eip63EC107 *F FBti0037928 PBac{3HPy+}enokC376 *F FBti0037826 PBac{3HPy+}fruC009 *F FBti0037903 PBac{3HPy+}Hsp67BaC291 *F FBti0037830 PBac{3HPy+}hydC017 *F FBti0037924 PBac{3HPy+}I-2C362 *F FBti0037860 PBac{3HPy+}l(3)IX-14C128 *F FBti0037898 PBac{3HPy+}LeucokininC275 *F FBti0037884 PBac{3HPy+}msiC220 *F FBti0037918 PBac{3HPy+}OsbpC347 *F FBti0037892 PBac{3HPy+}recC252 *F FBti0037876 PBac{3HPy+}RfaBpC204 *F FBti0037927 PBac{3HPy+}RglC370 *F FBti0037868 PBac{3HPy+}RimC165 *F FBti0037917 PBac{3HPy+}RpL8C345 *F FBti0037920 PBac{3HPy+}ShabC349 *F FBti0037823 PBac{3HPy+}staiC002 *F FBti0037888 PBac{3HPy+}Strn-MlckC234 *F FBti0037916 PBac{3HPy+}Taf2C341 *F FBti0037854 PBac{3HPy+}TieC098 *F FBti0037634 PBac{5HPw+}Cdk8A162 *F FBti0037738 PBac{5HPw+}CecBB193 *F FBti0037666 PBac{5HPw+}CG15438A311 *F FBti0037663 PBac{5HPw+}Cht4A307 *F FBti0037662 PBac{5HPw+}CkIIalpha-i3A288 *F FBti0037625 PBac{5HPw+}CycJA138 *F FBti0037731 PBac{5HPw+}DgB148 *F FBti0037661 PBac{5HPw+}dj-1betaA286 *F FBti0037772 PBac{5HPw+}dlpB313 *F FBti0037820 PBac{5HPw+}DNaseIIB571 *F FBti0037665 PBac{5HPw+}Eip63EA310 *F FBti0037775 PBac{5HPw+}eyB320 *F FBti0037786 PBac{5HPw+}fat2B395 *F FBti0037780 PBac{5HPw+}Fps85DB363 *F FBti0037672 PBac{5HPw+}glA341 *F FBti0037785 PBac{5HPw+}GM130B394 *F FBti0037712 PBac{5HPw+}Hrb98DEA544 *F FBti0037682 PBac{5HPw+}janAA392 *F FBti0037671 PBac{5HPw+}kluA340 *F FBti0037784 PBac{5HPw+}koB392 *F FBti0037638 PBac{5HPw+}ldsA190 *F FBti0037598 PBac{5HPw+}mei-P22A054 *F FBti0037796 PBac{5HPw+}mRpS26B451 *F FBti0037797 PBac{5HPw+}msiB455 *F FBti0037737 PBac{5HPw+}mskB185 *F FBti0037594 PBac{5HPw+}mus205A034 *F FBti0037718 PBac{5HPw+}ND23B117 *F FBti0037619 PBac{5HPw+}Or45bA120 *F FBti0037815 PBac{5HPw+}OscpB532 *F FBti0037770 PBac{5HPw+}ospB307 *F FBti0037763 PBac{5HPw+}OteB279 *F FBti0037787 PBac{5HPw+}PH4alphaNE1B400 *F FBti0037653 PBac{5HPw+}Plc21CA246 *F FBti0037622 PBac{5HPw+}PP2A-B'A131 *F FBti0037670 PBac{5HPw+}Pros26.4A336 *F FBti0037717 PBac{5HPw+}Ptp61FB106 *F FBti0037749 PBac{5HPw+}Rab8B229 *F FBti0037588 PBac{5HPw+}Ras85DA014 *F FBti0037688 PBac{5HPw+}sdaA427 *F FBti0037643 PBac{5HPw+}slmbA197 *F FBti0037818 PBac{5HPw+}Taf6LB560 *F FBti0037681 PBac{5HPw+}wdpA391 # *U FBrf0179797 *a Bloomington Drosophila Stock Center *b ?. *t 2004.11.6 *T personal communication to FlyBase *u Exelixis insertion alleles that are not in FlyBase. *F Date: Sat, 6 Nov 2004 19:47:59 \-0500 (EST) *F From: Kathy Matthews *F Subject: Exelixis insertion alleles that are not in FlyBase *F To: flybase-updates@morgan.harvard.edu *F Personal Communication from: Bloomington Drosophila Stock Center *F Subject: Exelixis insertion alleles that are not in FlyBase *F Dated: 6 Nov 2004 *F The 1,894 insertions listed below were donated to the Bloomington *F Stock Center by Exelixis, Inc. Insertion site coordinates for these *F insertions are listed in Thibault et al., Nature Genetics 36, *F 283 \- 287 (2004). The consequent alleles determined by Exelixis for *F these insertions were not enumerated in Thibault et al. and thus *F were not curated by FlyBase. To avoid further asynchrony between *F the Stock Center and FlyBase, we are passing this information, *F which was produced by Exelixis, along for curation. *F Subsequent to the publication of Thibault et al, 2004, Roger Hoskins *F and Joe Carlson of the Gene Disruption Project (GDP) re-analyzed *F Exelixis' sequence files. They have revised genome coordinates for *F many of these insertion sites. When their analysis is complete they *F will submit their results to FlyBase and the Stock Center. Some of *F the Exelixis allele calls listed below will almost certainly be *F incorrect based on the GDP analysis. Thus, users of these lines *F should proceed with caution. *F The three columns below are the FlyBase Insertion Identifier, the *F Insertion Symbol, and the Allele Phenotype. Where no Allele *F Phenotype is indicated, the insertion chromosome is balanced in *F stock, and thus is either lethal or sterile. It is not known (to us, *F at least) whether the insertion or some other lesion on the *F chromosome is responsible for the phenotype. PBac{RB}pxe02948 also *F has a classic plexus wing vein phenotype. *F FlyBase ID Insertion Symbol Allele Phenotype *F FBti0040876 PBac{PB}Aats-metc00449 *F FBti0040852 PBac{PB}Acec00215 *F FBti0041280 PBac{PB}Alg10c06300 *F FBti0041267 PBac{PB}Aos1c06048 *F FBti0041092 PBac{PB}Apc2c03232 *F FBti0041231 PBac{PB}Awhc05541 *F FBti0041344 PBac{PB}Brfc07161 *F FBti0041232 PBac{PB}Broc05542 viable and fertile *F FBti0041149 PBac{PB}CG10077c04490 viable and fertile *F FBti0040962 PBac{PB}CG10139c01615 *F FBti0041010 PBac{PB}CG10154c02170 *F FBti0041268 PBac{PB}CG10191c06059 viable and fertile *F FBti0040937 PBac{PB}CG10326c01273 viable and fertile *F FBti0040967 PBac{PB}CG10345c01661 viable and fertile *F FBti0041277 PBac{PB}CG10420c06294 *F FBti0041194 PBac{PB}CG10627c04986 *F FBti0041161 PBac{PB}CG10629c04626 viable and fertile *F FBti0040871 PBac{PB}CG10635c00409 *F FBti0040856 PBac{PB}CG10657c00236 viable and fertile *F FBti0041182 PBac{PB}CG10663c04898 viable and fertile *F FBti0041203 PBac{PB}CG10898c05103 viable and fertile *F FBti0040956 PBac{PB}CG1092c01553 viable and fertile *F FBti0041046 PBac{PB}CG10948c02658 viable and fertile *F FBti0041023 PBac{PB}CG10959c02347 viable and fertile *F FBti0041121 PBac{PB}CG11006c03966 viable and fertile *F FBti0041099 PBac{PB}CG11030c03484 *F FBti0040971 PBac{PB}CG11037c01705 viable and fertile *F FBti0041138 PBac{PB}CG33291c04287 viable and fertile *F FBti0041273 PBac{PB}CG11261c06238 viable and fertile *F FBti0040957 PBac{PB}CG11293c01555 viable and fertile *F FBti0041174 PBac{PB}CG11309c04798 viable and fertile *F FBti0040925 PBac{PB}CG11317c01160 viable and fertile *F FBti0041199 PBac{PB}CG11375c05043 *F FBti0041084 PBac{PB}CG11391c03091 viable and fertile *F FBti0040974 PBac{PB}CG11413c01733 viable and fertile *F FBti0040862 PBac{PB}CG11489c00270 viable and fertile *F FBti0041207 PBac{PB}CG1148c05210 viable and fertile *F FBti0040959 PBac{PB}CG11843c01611 viable and fertile *F FBti0040996 PBac{PB}CG11851c02021 *F FBti0040869 PBac{PB}CG11892c00398 viable and fertile *F FBti0041106 PBac{PB}CG11893c03576 viable and fertile *F FBti0041144 PBac{PB}CG11927c04401 viable and fertile *F FBti0040934 PBac{PB}CG1193c01236 viable and fertile *F FBti0040981 PBac{PB}CG1213c01845 viable and fertile *F FBti0041241 PBac{PB}CG12338c05692 viable and fertile *F FBti0041070 PBac{PB}CG12413c02868 *F FBti0041094 PBac{PB}CG12492c03287 viable and fertile *F FBti0041278 PBac{PB}CG1273c06297 viable and fertile *F FBti0041176 PBac{PB}CG12842c04811 viable and fertile *F FBti0041287 PBac{PB}CG12869c06468 viable and fertile *F FBti0041179 PBac{PB}CG12913c04874 *F FBti0040980 PBac{PB}CG13039c01810 viable and fertile *F FBti0040878 PBac{PB}CG13053c00487 viable and fertile *F FBti0041302 PBac{PB}CG1324c06609 viable and fertile *F FBti0041160 PBac{PB}CG13255c04618 viable and fertile *F FBti0040923 PBac{PB}CG13258c01131 viable and fertile *F FBti0040916 PBac{PB}CG13318c01061 viable and fertile *F FBti0041228 PBac{PB}CG13618c05519 viable and fertile *F FBti0040863 PBac{PB}CG13620c00300 viable and fertile *F FBti0041170 PBac{PB}CG13640c04775 viable and fertile *F FBti0041150 PBac{PB}CG13699c04491 viable and fertile *F FBti0041248 PBac{PB}CG13744c05793 viable and fertile *F FBti0041311 PBac{PB}CG13776c06748 viable and fertile *F FBti0041265 PBac{PB}CG13860c05998 viable and fertile *F FBti0040966 PBac{PB}CG13890c01654 viable and fertile *F FBti0041155 PBac{PB}CG13914c04570 viable and fertile *F FBti0041151 PBac{PB}CG14030c04512 viable and fertile *F FBti0041096 PBac{PB}CG14165c03353 viable and fertile *F FBti0040993 PBac{PB}CG14282c01997 viable and fertile *F FBti0040936 PBac{PB}CG14470c01263 viable and fertile *F FBti0041115 PBac{PB}CG14506c03887 viable and fertile *F FBti0040904 PBac{PB}CG14507c00851 viable and fertile *F FBti0040930 PBac{PB}CG14518c01206 viable and fertile *F FBti0041244 PBac{PB}CG14635c05722 viable and fertile *F FBti0041284 PBac{PB}CG14669c06402 viable and fertile *F FBti0041172 PBac{PB}CG14686c04788 viable and fertile *F FBti0041052 PBac{PB}CG14687c02737 viable and fertile *F FBti0041178 PBac{PB}CG14731c04833 viable and fertile *F FBti0040970 PBac{PB}CG14889c01692 viable and fertile *F FBti0040844 PBac{PB}CG14906c00109 viable and fertile *F FBti0041077 PBac{PB}CG14955c02957 *F FBti0040899 PBac{PB}CG14959c00801 viable and fertile *F FBti0040942 PBac{PB}CG14968c01408 viable and fertile *F FBti0041018 PBac{PB}CG14971c02275 viable and fertile *F FBti0041053 PBac{PB}CG14971c02743 viable and fertile *F FBti0041324 PBac{PB}CG1499c06953 *F FBti0041044 PBac{PB}CG15013c02647 viable and fertile *F FBti0040911 PBac{PB}CG15020c01022 viable and fertile *F FBti0041120 PBac{PB}CG15021c03960 viable and fertile *F FBti0040968 PBac{PB}CG15120c01671 viable and fertile *F FBti0040950 PBac{PB}CG15432c01492 viable and fertile *F FBti0041042 PBac{PB}CG15484c02598 *F FBti0041074 PBac{PB}CG15524c02901 viable and fertile *F FBti0040912 PBac{PB}CG15628c01024 viable and fertile *F FBti0041130 PBac{PB}CG15695c04218 *F FBti0041192 PBac{PB}CG15696c04976 *F FBti0041105 PBac{PB}CG15715c03573 viable and fertile *F FBti0041238 PBac{PB}CG15817c05664 viable and fertile *F FBti0041205 PBac{PB}CG15890c05173 viable and fertile *F FBti0041236 PBac{PB}CG1638c05632 viable and fertile *F FBti0040845 PBac{PB}CG16799c00121 viable and fertile *F FBti0041221 PBac{PB}CG16935c05454 viable and fertile *F FBti0041250 PBac{PB}CG16957c05832 viable and fertile *F FBti0041003 PBac{PB}CG16965c02094 viable and fertile *F FBti0041104 PBac{PB}CG17227c03514 viable and fertile *F FBti0041321 PBac{PB}CG17257c06930 viable and fertile *F FBti0040881 PBac{PB}CG17262c00569 *F FBti0041252 PBac{PB}CG17331c05852 viable and fertile *F FBti0041079 PBac{PB}CG17343c02988 viable and fertile *F FBti0040860 PBac{PB}CG17352c00268 viable and fertile *F FBti0041220 PBac{PB}CG17361c05438 viable and fertile *F FBti0041165 PBac{PB}CG17568c04713 viable and fertile *F FBti0041190 PBac{PB}CG17612c04955 viable and fertile *F FBti0041143 PBac{PB}CG17838c04375 *F FBti0041198 PBac{PB}CG17919c05035 viable and fertile *F FBti0041229 PBac{PB}CG18178c05537 viable and fertile *F FBti0041008 PBac{PB}CG18314c02142 *F FBti0041017 PBac{PB}CG18619c02273 viable and fertile *F FBti0041091 PBac{PB}CG18769c03230 viable and fertile *F FBti0040997 PBac{PB}CG1890c02022 viable and fertile *F FBti0041195 PBac{PB}CG1902c04990 viable and fertile *F FBti0041152 PBac{PB}CG1906c04528 viable and fertile *F FBti0040946 PBac{PB}CG1941c01452 viable and fertile *F FBti0041029 PBac{PB}CG2082c02462 viable and fertile *F FBti0041298 PBac{PB}CG2493c06582 viable and fertile *F FBti0041305 PBac{PB}CG2508c06630 viable and fertile *F FBti0041051 PBac{PB}CG2657c02720 viable and fertile *F FBti0041088 PBac{PB}CG2698c03200 viable and fertile *F FBti0041048 PBac{PB}CG2747c02682 *F FBti0040954 PBac{PB}CG2919c01524 viable and fertile *F FBti0040892 PBac{PB}CG2950c00722 *F FBti0041295 PBac{PB}CG30161c06534 *F FBti0040914 PBac{PB}CG30194c01052 viable and fertile *F FBti0040875 PBac{PB}CG30383c00440 viable and fertile *F FBti0040943 PBac{PB}CG30392c01436 viable and fertile *F FBti0041196 PBac{PB}CG30393c05001 viable and fertile *F FBti0041281 PBac{PB}CG30442c06370 viable and fertile *F FBti0041177 PBac{PB}CG31005c04819 *F FBti0040894 PBac{PB}CG31100c00745 viable and fertile *F FBti0040842 PBac{PB}CG31156c00090 viable and fertile *F FBti0040907 PBac{PB}CG31158c00953 viable and fertile *F FBti0040857 PBac{PB}CG31177c00239 viable and fertile *F FBti0041043 PBac{PB}CG31268c02630 viable and fertile *F FBti0041271 PBac{PB}CG31326c06215 viable and fertile *F FBti0040944 PBac{PB}CG31357c01438 viable and fertile *F FBti0040991 PBac{PB}CG31367c01987 viable and fertile *F FBti0041200 PBac{PB}CG3136c05057 viable and fertile *F FBti0040978 PBac{PB}CG31370c01792 viable and fertile *F FBti0040913 PBac{PB}CG31465c01035 viable and fertile *F FBti0040979 PBac{PB}CG31730c01798 viable and fertile *F FBti0041025 PBac{PB}CG31902c02416 *F FBti0040838 PBac{PB}CG31935c00037 viable and fertile *F FBti0041124 PBac{PB}CG31973c04017 viable and fertile *F FBti0041258 PBac{PB}CG32029c05937 viable and fertile *F FBti0041054 PBac{PB}CG3203c02747 viable and fertile *F FBti0040960 PBac{PB}CG32085c01612 *F FBti0041209 PBac{PB}CG32089c05245 viable and fertile *F FBti0041156 PBac{PB}CG32133c04574 *F FBti0040919 PBac{PB}CG32206c01093 viable and fertile *F FBti0041188 PBac{PB}CG32248c04942 viable and fertile *F FBti0040921 PBac{PB}CG32253c01124 *F FBti0041234 PBac{PB}CG32260c05553 *F FBti0040900 PBac{PB}CG3231c00805 viable and fertile *F FBti0041036 PBac{PB}CG32341c02538 viable and fertile *F FBti0041317 PBac{PB}CG32372c06895 viable and fertile *F FBti0041279 PBac{PB}CG32373c06299 *F FBti0041117 PBac{PB}CG32409c03942 *F FBti0040955 PBac{PB}CG32428c01528 viable and fertile *F FBti0040995 PBac{PB}CG3244c02010 viable and fertile *F FBti0041110 PBac{PB}CG32625c03754 viable and fertile *F FBti0041247 PBac{PB}CG32803c05749 viable and fertile *F FBti0041259 PBac{PB}CG32912c05953 viable and fertile *F FBti0041024 PBac{PB}CG33051c02392 viable and fertile *F FBti0041243 PBac{PB}CG33085c05706 viable and fertile *F FBti0040901 PBac{PB}CG33123c00827 *F FBti0041089 PBac{PB}CG33123c03210 *F FBti0041218 PBac{PB}CG33137c05413 *F FBti0041146 PBac{PB}CG33142c04444 viable and fertile *F FBti0040855 PBac{PB}CG3321c00226 viable and fertile *F FBti0041100 PBac{PB}CG33275c03487 viable and fertile *F FBti0041260 PBac{PB}CG3335c05958 *F FBti0041006 PBac{PB}CG3485c02114 viable and fertile *F FBti0041057 PBac{PB}CG3590c02781 *F FBti0041299 PBac{PB}CG3672c06585 viable and fertile *F FBti0041339 PBac{PB}CG3983c07050 *F FBti0041235 PBac{PB}CG4022c05627 viable and fertile *F FBti0041313 PBac{PB}CG4040c06792 viable and fertile *F FBti0041216 PBac{PB}CG4115c05379 viable and fertile *F FBti0041095 PBac{PB}CG4360c03323 viable and fertile *F FBti0040915 PBac{PB}CG4452c01056 viable and fertile *F FBti0040902 PBac{PB}CG4462c00832 viable and fertile *F FBti0041012 PBac{PB}CG4465c02189 viable and fertile *F FBti0041001 PBac{PB}CG4554c02084 *F FBti0041240 PBac{PB}CG4565c05681 viable and fertile *F FBti0041261 PBac{PB}CG4572c05963 viable and fertile *F FBti0041040 PBac{PB}CG4622c02584 viable and fertile *F FBti0041090 PBac{PB}CG4683c03216 viable and fertile *F FBti0041341 PBac{PB}CG4757c07133 *F FBti0040984 PBac{PB}CG4759c01874 *F FBti0041059 PBac{PB}CG4818c02802 viable and fertile *F FBti0041132 PBac{PB}CG4836c04238 *F FBti0041061 PBac{PB}CG4851c02813 viable and fertile *F FBti0041184 PBac{PB}CG4861c04916 viable and fertile *F FBti0041041 PBac{PB}CG4940c02594 viable and fertile *F FBti0041217 PBac{PB}CG5001c05411 viable and fertile *F FBti0041282 PBac{PB}CG5037c06394 viable and fertile *F FBti0041291 PBac{PB}CG5104c06516 viable and fertile *F FBti0040999 PBac{PB}CG5122c02042 viable and fertile *F FBti0041109 PBac{PB}CG5148c03646 *F FBti0040890 PBac{PB}CG5149c00700 viable and fertile *F FBti0041237 PBac{PB}CG5156c05647 viable and fertile *F FBti0040931 PBac{PB}CG5181c01211 viable and fertile *F FBti0040851 PBac{PB}CG5191c00205 viable and fertile *F FBti0041342 PBac{PB}CG5198c07150 *F FBti0041113 PBac{PB}CG5288c03848 viable and fertile *F FBti0041000 PBac{PB}CG5447c02058 viable and fertile *F FBti0041326 PBac{PB}CG5467c06968 viable and fertile *F FBti0041083 PBac{PB}CG5494c03081 viable and fertile *F FBti0040929 PBac{PB}CG5758c01197 *F FBti0041022 PBac{PB}CG5830c02324 viable and fertile *F FBti0041085 PBac{PB}CG5850c03122 viable and fertile *F FBti0040873 PBac{PB}CG5873c00427 viable and fertile *F FBti0041133 PBac{PB}CG5910c04241 viable and fertile *F FBti0041014 PBac{PB}CG5938c02218 viable and fertile *F FBti0041256 PBac{PB}CG5978c05919 viable and fertile *F FBti0041108 PBac{PB}CG6000c03607 *F FBti0041242 PBac{PB}CG6069c05699 viable and fertile *F FBti0041127 PBac{PB}CG6185c04139 viable and fertile *F FBti0041101 PBac{PB}CG6225c03494 *F FBti0040877 PBac{PB}CG6393c00465 viable and fertile *F FBti0040935 PBac{PB}CG6488c01259 viable and fertile *F FBti0041159 PBac{PB}CG6563c04615 viable and fertile *F FBti0040906 PBac{PB}CG6688c00937 viable and fertile *F FBti0040958 PBac{PB}CG6729c01575 viable and fertile *F FBti0041301 PBac{PB}CG6752c06604 viable and fertile *F FBti0041272 PBac{PB}CG6792c06236 viable and fertile *F FBti0041114 PBac{PB}CG6833c03857 viable and fertile *F FBti0041019 PBac{PB}CG6999c02279 viable and fertile *F FBti0040988 PBac{PB}CG7015c01923 viable and fertile *F FBti0041292 PBac{PB}CG7029c06518 *F FBti0041034 PBac{PB}CG7120c02508 viable and fertile *F FBti0041038 PBac{PB}CG7202c02567 viable and fertile *F FBti0040891 PBac{PB}CG7207c00719 viable and fertile *F FBti0040961 PBac{PB}CG7214c01614 viable and fertile *F FBti0041026 PBac{PB}CG7272c02435 viable and fertile *F FBti0041193 PBac{PB}CG7394c04985 *F FBti0041027 PBac{PB}CG7402c02441 viable and fertile *F FBti0040893 PBac{PB}CG7414c00723 viable and fertile *F FBti0041185 PBac{PB}CG7514c04924 viable and fertile *F FBti0041210 PBac{PB}CG7549c05288 *F FBti0041325 PBac{PB}CG7573c06954 viable and fertile *F FBti0041275 PBac{PB}CG7593c06246 viable and fertile *F FBti0041314 PBac{PB}CG7735c06844 viable and fertile *F FBti0041066 PBac{PB}CG7814c02849 viable and fertile *F FBti0041329 PBac{PB}CG7834c06979 viable and fertile *F FBti0040903 PBac{PB}CG7845c00845 *F FBti0041257 PBac{PB}CG7879c05931 viable and fertile *F FBti0041315 PBac{PB}CG8036c06882 viable and fertile *F FBti0041254 PBac{PB}CG8064c05886 *F FBti0040868 PBac{PB}CG8092c00387 viable and fertile *F FBti0041147 PBac{PB}CG8129c04459 viable and fertile *F FBti0040975 PBac{PB}CG8207c01762 viable and fertile *F FBti0041245 PBac{PB}CG8301c05730 viable and fertile *F FBti0041060 PBac{PB}CG8359c02812 viable and fertile *F FBti0041131 PBac{PB}CG8400c04227 viable and fertile *F FBti0041340 PBac{PB}CG8538c07132 viable and fertile *F FBti0041123 PBac{PB}CG8552c03991 *F FBti0041112 PBac{PB}CG8760c03838 viable and fertile *F FBti0040850 PBac{PB}CG8778c00172 viable and fertile *F FBti0040927 PBac{PB}CG8910c01167 viable and fertile *F FBti0040945 PBac{PB}CG9122c01440 viable and fertile *F FBti0041197 PBac{PB}CG9175c05024 viable and fertile *F FBti0040976 PBac{PB}CG9196c01763 viable and fertile *F FBti0041183 PBac{PB}CG9307c04908 viable and fertile *F FBti0041297 PBac{PB}CG9320c06563 viable and fertile *F FBti0041050 PBac{PB}CG9619c02710 viable and fertile *F FBti0040864 PBac{PB}CG9664c00321 viable and fertile *F FBti0041187 PBac{PB}CG9684c04938 viable and fertile *F FBti0041215 PBac{PB}CG9850c05320 viable and fertile *F FBti0040848 PBac{PB}CG9932c00144 *F FBti0041072 PBac{PB}CHORDc02881 *F FBti0040940 PBac{PB}Caf1-105c01369 viable and fertile *F FBti0041312 PBac{PB}Ced-12c06760 *F FBti0041134 PBac{PB}Cskc04256 viable and fertile *F FBti0041212 PBac{PB}CycAc05304 *F FBti0041276 PBac{PB}Cyp4c3c06288 *F FBti0040948 PBac{PB}Cyp4s3c01459 viable and fertile *F FBti0041263 PBac{PB}Dripc05976 viable and fertile *F FBti0041334 PBac{PB}Dysc07024 viable and fertile *F FBti0041310 PBac{PB}Espc06746 viable and fertile *F FBti0041118 PBac{PB}GRHRc03956 viable and fertile *F FBti0041253 PBac{PB}Galpha73Bc05874 viable and fertile *F FBti0040985 PBac{PB}Gr28bc01884 viable and fertile *F FBti0041285 PBac{PB}IM4c06403 viable and fertile *F FBti0041214 PBac{PB}Irisc05310 viable and fertile *F FBti0041227 PBac{PB}Irisc05504 viable and fertile *F FBti0041020 PBac{PB}Klcc02312 *F FBti0041186 PBac{PB}Lmptc04937 viable and fertile *F FBti0040986 PBac{PB}NPFR1c01896 viable and fertile *F FBti0040952 PBac{PB}NUCB1c01508 viable and fertile *F FBti0041065 PBac{PB}Nep4c02841 *F FBti0040924 PBac{PB}NepYrc01146 viable and fertile *F FBti0040990 PBac{PB}Neu3c01955 *F FBti0040884 PBac{PB}Nf1c00617 viable and fertile *F FBti0041015 PBac{PB}O-fut1c02264 viable and fertile *F FBti0040973 PBac{PB}Optixc01718 *F FBti0041128 PBac{PB}PDCD-5c04145 *F FBti0041148 PBac{PB}Pde1cc04487 viable and fertile *F FBti0041004 PBac{PB}Pi4KIIalphac02099 viable and fertile *F FBti0041327 PBac{PB}Pka-R1c06969 viable and fertile *F FBti0040858 PBac{PB}Plc21Cc00245 viable and fertile *F FBti0041166 PBac{PB}Proctc04750 viable and fertile *F FBti0041343 PBac{PB}Prp18c07155 viable and fertile *F FBti0041211 PBac{PB}Ptp61Fc05292 *F FBti0041013 PBac{PB}Pvrc02195 *F FBti0041033 PBac{PB}Rfxc02503 viable and fertile *F FBti0040922 PBac{PB}RhoGAP100Fc01128 *F FBti0041045 PBac{PB}Rlipc02656 viable and fertile *F FBti0040939 PBac{PB}RpA-70c01306 viable and fertile *F FBti0040969 PBac{PB}Rpb11c01686 *F FBti0041021 PBac{PB}Rpb7c02314 viable and fertile *F FBti0040889 PBac{PB}Rrp1c00695 *F FBti0041303 PBac{PB}SAKc06612 *F FBti0040870 PBac{PB}SMC1c00402 viable and fertile *F FBti0041274 PBac{PB}SP1173c06239 viable and fertile *F FBti0041037 PBac{PB}Sbc02563 *F FBti0040933 PBac{PB}Scsalphac01235 viable and fertile *F FBti0040866 PBac{PB}SdhBc00364 *F FBti0040932 PBac{PB}Spn5c01214 *F FBti0041164 PBac{PB}Src64Bc04709 *F FBti0041068 PBac{PB}Strn-Mlckc02860 *F FBti0041145 PBac{PB}Su(fu)c04425 *F FBti0041122 PBac{PB}T-cp1c03987 *F FBti0040972 PBac{PB}TFAMc01716 *F FBti0041239 PBac{PB}Toll-9c05666 viable and fertile *F FBti0041030 PBac{PB}Tsp42Epc02475 viable and fertile *F FBti0041262 PBac{PB}Ugt58Fac05973 *F FBti0040872 PBac{PB}Vap-33-1c00426 viable and fertile *F FBti0041202 PBac{PB}alpha-Est3c05084 viable and fertile *F FBti0041283 PBac{PB}beat-VIc06401 *F FBti0041225 PBac{PB}borc05496 *F FBti0041098 PBac{PB}calsc03483 viable and fertile *F FBti0040874 PBac{PB}cbxc00428 viable and fertile *F FBti0041102 PBac{PB}cdc2c03495 *F FBti0040883 PBac{PB}clc00597 viable and fertile *F FBti0040849 PBac{PB}dc00148 viable and fertile *F FBti0041158 PBac{PB}dboc04604 viable and fertile *F FBti0040977 PBac{PB}dsc01777 viable and fertile *F FBti0041011 PBac{PB}dyn-p25c02174 *F FBti0041153 PBac{PB}eIF-1Ac04533 *F FBti0040994 PBac{PB}eIF2B-betac02002 *F FBti0040989 PBac{PB}eIF2B-gammac01931 *F FBti0040909 PBac{PB}elBc01007 viable and fertile *F FBti0041213 PBac{PB}eygc05307 viable and fertile *F FBti0041167 PBac{PB}fanc04756 viable and fertile *F FBti0040837 PBac{PB}grkc00007 *F FBti0041294 PBac{PB}hbsc06523 viable and fertile *F FBti0041076 PBac{PB}hepc02950 *F FBti0041336 PBac{PB}huntingtinc07030 viable and fertile *F FBti0041002 PBac{PB}inc02090 viable and fertile *F FBti0040949 PBac{PB}jdpc01474 viable and fertile *F FBti0041064 PBac{PB}keyc02831 viable and fertile *F FBti0040951 PBac{PB}krzc01503 *F FBti0041173 PBac{PB}mRpL51c04791 *F FBti0040887 PBac{PB}mRpL9c00642 *F FBti0040987 PBac{PB}masc01899 *F FBti0041157 PBac{PB}mex1c04603 viable and fertile *F FBti0041169 PBac{PB}miple2c04774 viable and fertile *F FBti0041269 PBac{PB}nbsc06100 *F FBti0040992 PBac{PB}omec01991 viable and fertile *F FBti0040839 PBac{PB}parkc00062 viable and fertile *F FBti0041111 PBac{PB}ppk23c03836 viable and fertile *F FBti0041097 PBac{PB}pugc03481 *F FBti0040947 PBac{PB}rho-5c01455 viable and fertile *F FBti0041189 PBac{PB}scaf6c04944 viable and fertile *F FBti0041028 PBac{PB}sec31c02461 *F FBti0040897 PBac{PB}spn-Ec00786 *F FBti0040964 PBac{PB}staic01639 *F FBti0041201 PBac{PB}tauc05068 viable and fertile *F FBti0041069 PBac{PB}thoc02867 viable and fertile *F FBti0041328 PBac{PB}timeoutc06976 viable and fertile *F FBti0040885 PBac{PB}toc00632 viable and fertile *F FBti0040888 PBac{PB}yellow-ec00645 viable and fertile *F FBti0041073 PBac{PB}yip3c02887 viable and fertile *F FBti0041126 PBac{PB}zwilchc04101 viable and fertile *F FBti0041406 PBac{RB}A16e00533 viable and fertile *F FBti0041832 PBac{RB}AP-1gammae04546 *F FBti0041833 PBac{RB}Aats-lyse04554 *F FBti0041352 PBac{RB}Aats-proe00080 *F FBti0041453 PBac{RB}Brfe00975 *F FBti0041457 PBac{RB}Brucee00984 *F FBti0041531 PBac{RB}CG10063e01749 viable and fertile *F FBti0041535 PBac{RB}CG10083e01789 viable and fertile *F FBti0041745 PBac{RB}CG10105e03756 viable and fertile *F FBti0041779 PBac{RB}CG10112e03998 *F FBti0041505 PBac{RB}CG10175e01487 viable and fertile *F FBti0041825 PBac{RB}CG10361e04492 viable and fertile *F FBti0041665 PBac{RB}CG10414e03046 viable and fertile *F FBti0041502 PBac{RB}CG1041e01463 viable and fertile *F FBti0041840 PBac{RB}CG10555e04596 *F FBti0041547 PBac{RB}CG10628e01892 *F FBti0041462 PBac{RB}CG10638e01020 viable and fertile *F FBti0041742 PBac{RB}CG10688e03723 viable and fertile *F FBti0041612 PBac{RB}CG10695e02477 viable and fertile *F FBti0041638 PBac{RB}CG1077e02817 viable and fertile *F FBti0041749 PBac{RB}CG10924e03788 viable and fertile *F FBti0041849 PBac{RB}CG10936e04671 viable and fertile *F FBti0041555 PBac{RB}CG10999e01961 viable and fertile *F FBti0041731 PBac{RB}CG11063e03614 viable and fertile *F FBti0041425 PBac{RB}CG11073e00717 viable and fertile *F FBti0041400 PBac{RB}CG11175e00423 viable and fertile *F FBti0041770 PBac{RB}CG11180e03938 *F FBti0041666 PBac{RB}CG11188e03057 *F FBti0041797 PBac{RB}CG11242e04210 viable and fertile *F FBti0041757 PBac{RB}CG11318e03837 viable and fertile *F FBti0041369 PBac{RB}CG11340e00225 viable and fertile *F FBti0041698 PBac{RB}CG11378e03299 viable and fertile *F FBti0041810 PBac{RB}CG11403e04332 viable and fertile *F FBti0041467 PBac{RB}CG11419e01070 *F FBti0041831 PBac{RB}CG11436e04544 viable and fertile *F FBti0041824 PBac{RB}CG11526e04482 viable and fertile *F FBti0041685 PBac{RB}CG11577e03196 viable and fertile *F FBti0041403 PBac{RB}CG11617e00462 viable and fertile *F FBti0041787 PBac{RB}CG11674e04146 viable and fertile *F FBti0041813 PBac{RB}CG11752e04370 viable and fertile *F FBti0041652 PBac{RB}CG11838e02928 viable and fertile *F FBti0041585 PBac{RB}CG11842e02284 viable and fertile *F FBti0041511 PBac{RB}CG11896e01527 viable and fertile *F FBti0041728 PBac{RB}CG11898e03595 viable and fertile *F FBti0041370 PBac{RB}CG12090e00227 viable and fertile *F FBti0041456 PBac{RB}CG12099e00982 viable and fertile *F FBti0041729 PBac{RB}CG12119e03600 viable and fertile *F FBti0041607 PBac{RB}CG12123e02426 viable and fertile *F FBti0041546 PBac{RB}CG12133e01891 viable and fertile *F FBti0041622 PBac{RB}CG12134e02634 viable and fertile *F FBti0041595 PBac{RB}CG12151e02351 viable and fertile *F FBti0041596 PBac{RB}CG12179e02353 viable and fertile *F FBti0041714 PBac{RB}CG12255e03478 viable and fertile *F FBti0041709 PBac{RB}CG12279e03416 viable and fertile *F FBti0041780 PBac{RB}CG12344e04018 viable and fertile *F FBti0041506 PBac{RB}CG1234e01488 *F FBti0041716 PBac{RB}CG12360e03481 viable and fertile *F FBti0041449 PBac{RB}CG12391e00914 viable and fertile *F FBti0041410 PBac{RB}CG12576e00577 viable and fertile *F FBti0041614 PBac{RB}CG1259e02505 *F FBti0041527 PBac{RB}CG12717e01706 viable and fertile *F FBti0041613 PBac{RB}CG12724e02492 viable and fertile *F FBti0041821 PBac{RB}CG1273e04471 viable and fertile *F FBti0041633 PBac{RB}CG12783e02761 viable and fertile *F FBti0041362 PBac{RB}CG12854e00165 viable and fertile *F FBti0041775 PBac{RB}CG12934e03982 *F FBti0041407 PBac{RB}CG13018e00535 *F FBti0041658 PBac{RB}CG13031e02987 viable and fertile *F FBti0041721 PBac{RB}CG13035e03531 viable and fertile *F FBti0041554 PBac{RB}CG13043e01947 viable and fertile *F FBti0041618 PBac{RB}CG13319e02594 viable and fertile *F FBti0041801 PBac{RB}CG13333e04231 viable and fertile *F FBti0041488 PBac{RB}CG13379e01308 *F FBti0041548 PBac{RB}CG13531e01893 viable and fertile *F FBti0041774 PBac{RB}CG13551e03979 *F FBti0041744 PBac{RB}CG13680e03740 *F FBti0041427 PBac{RB}CG13689e00773 viable and fertile *F FBti0041669 PBac{RB}CG13852e03077 *F FBti0041479 PBac{RB}CG13902e01240 viable and fertile *F FBti0041615 PBac{RB}CG14015e02545 viable and fertile *F FBti0041795 PBac{RB}CG14023e04200 *F FBti0041590 PBac{RB}CG14047e02316 viable and fertile *F FBti0041720 PBac{RB}CG14057e03520 *F FBti0041374 PBac{RB}CG14117e00256 viable and fertile *F FBti0041528 PBac{RB}CG14212e01721 viable and fertile *F FBti0041450 PBac{RB}CG14286e00961 viable and fertile *F FBti0041740 PBac{RB}CG14301e03694 viable and fertile *F FBti0041743 PBac{RB}CG14303e03728 viable and fertile *F FBti0041695 PBac{RB}CG14304e03260 viable and fertile *F FBti0041766 PBac{RB}CG14435e03914 viable and fertile *F FBti0041366 PBac{RB}CG1443e00199 viable and fertile *F FBti0041552 PBac{RB}CG14545e01934 viable and fertile *F FBti0041485 PBac{RB}CG14598e01297 *F FBti0041823 PBac{RB}CG14655e04480 *F FBti0041476 PBac{RB}CG14675e01184 viable and fertile *F FBti0041507 PBac{RB}CG14695e01492 viable and fertile *F FBti0041388 PBac{RB}CG14830e00332 *F FBti0041661 PBac{RB}CG14838e03004 viable and fertile *F FBti0041541 PBac{RB}CG14885e01821 viable and fertile *F FBti0041627 PBac{RB}CG15011e02675 viable and fertile *F FBti0041478 PBac{RB}CG15012e01226 viable and fertile *F FBti0041351 PBac{RB}CG15096e00079 viable and fertile *F FBti0041659 PBac{RB}CG15118e03002 viable and fertile *F FBti0041777 PBac{RB}CG15261e03986 *F FBti0041700 PBac{RB}CG15369e03306 viable and fertile *F FBti0041756 PBac{RB}CG15443e03827 viable and fertile *F FBti0041672 PBac{RB}CG15544e03116 *F FBti0041674 PBac{RB}CG15556e03142 viable and fertile *F FBti0041368 PBac{RB}CG15597e00223 viable and fertile *F FBti0041754 PBac{RB}CG15625e03818 viable and fertile *F FBti0041793 PBac{RB}CG15706e04194 viable and fertile *F FBti0041610 PBac{RB}CG15743e02449 viable and fertile *F FBti0041544 PBac{RB}CG1602e01855 viable and fertile *F FBti0041451 PBac{RB}CG1607e00971 *F FBti0041611 PBac{RB}CG1657e02476 viable and fertile *F FBti0041736 PBac{RB}CG16735e03669 viable and fertile *F FBti0041765 PBac{RB}CG1677e03912 viable and fertile *F FBti0041755 PBac{RB}CG16786e03819 *F FBti0041473 PBac{RB}CG16833e01119 *F FBti0041566 PBac{RB}CG16890e02098 viable and fertile *F FBti0041761 PBac{RB}CG16892e03860 viable and fertile *F FBti0041460 PBac{RB}CG16908e01001 *F FBti0041750 PBac{RB}CG16952e03798 viable and fertile *F FBti0041497 PBac{RB}CG16953e01395 viable and fertile *F FBti0041657 PBac{RB}CG16979e02981 *F FBti0041619 PBac{RB}CG17086e02595 viable and fertile *F FBti0041808 PBac{RB}CG17333e04318 viable and fertile *F FBti0041739 PBac{RB}CG17350e03687 viable and fertile *F FBti0041587 PBac{RB}CG17360e02295 viable and fertile *F FBti0041690 PBac{RB}CG17560e03221 viable and fertile *F FBti0041422 PBac{RB}CG17597e00674 *F FBti0041523 PBac{RB}CG17600e01638 viable and fertile *F FBti0041812 PBac{RB}CG17788e04368 viable and fertile *F FBti0041802 PBac{RB}CG17977e04268 *F FBti0041592 PBac{RB}CG1839e02322 viable and fertile *F FBti0041597 PBac{RB}CG33248e02366 viable and fertile *F FBti0041846 PBac{RB}CG18606e04639 *F FBti0041737 PBac{RB}CG18616e03672 viable and fertile *F FBti0041481 PBac{RB}CG1939e01273 viable and fertile *F FBti0041703 PBac{RB}CG1961e03318 viable and fertile *F FBti0041589 PBac{RB}CG2076e02315 viable and fertile *F FBti0041408 PBac{RB}CG2082e00558 viable and fertile *F FBti0041378 PBac{RB}CG2103e00279 viable and fertile *F FBti0041492 PBac{RB}CG2191e01332 viable and fertile *F FBti0041601 PBac{RB}CG2247e02382 viable and fertile *F FBti0041468 PBac{RB}CG2249e01074 viable and fertile *F FBti0041609 PBac{RB}CG2258e02447 viable and fertile *F FBti0041806 PBac{RB}CG2371e04310 viable and fertile *F FBti0041835 PBac{RB}CG2467e04564 *F FBti0041704 PBac{RB}CG2658e03358 viable and fertile *F FBti0041650 PBac{RB}CG2736e02896 viable and fertile *F FBti0041644 PBac{RB}CG2767e02843 *F FBti0041624 PBac{RB}CG2921e02644 *F FBti0041509 PBac{RB}CG2926e01499 viable and fertile *F FBti0041513 PBac{RB}CG30023e01563 viable and fertile *F FBti0041514 PBac{RB}CG30094e01566 viable and fertile *F FBti0041417 PBac{RB}CG3009e00620 viable and fertile *F FBti0041545 PBac{RB}CG30100e01875 viable and fertile *F FBti0041572 PBac{RB}CG30259e02172 viable and fertile *F FBti0041443 PBac{RB}CG30387e00881 viable and fertile *F FBti0041751 PBac{RB}CG30414e03801 viable and fertile *F FBti0041521 PBac{RB}CG30431e01618 *F FBti0041404 PBac{RB}CG30502e00486 viable and fertile *F FBti0041375 PBac{RB}CG31120e00272 *F FBti0041508 PBac{RB}CG31211e01498 *F FBti0041679 PBac{RB}CG31278e03169 viable and fertile *F FBti0041785 PBac{RB}CG31337e04105 *F FBti0041367 PBac{RB}CG31368e00215 *F FBti0041466 PBac{RB}CG31369e01053 viable and fertile *F FBti0041783 PBac{RB}CG31448e04092 viable and fertile *F FBti0041792 PBac{RB}CG31460e04191 viable and fertile *F FBti0041796 PBac{RB}CG31542e04201 viable and fertile *F FBti0041655 PBac{RB}CG31738e02963 *F FBti0041662 PBac{RB}CG31855e03029 *F FBti0041361 PBac{RB}CG31878e00164 viable and fertile *F FBti0041769 PBac{RB}CG31898e03937 viable and fertile *F FBti0041608 PBac{RB}CG3191e02435 viable and fertile *F FBti0041565 PBac{RB}CG32016e02096 viable and fertile *F FBti0041553 PBac{RB}CG32091e01941 viable and fertile *F FBti0041487 PBac{RB}CG32104e01302 viable and fertile *F FBti0041491 PBac{RB}CG32158e01330 viable and fertile *F FBti0041448 PBac{RB}CG32176e00911 viable and fertile *F FBti0041639 PBac{RB}CG32209e02821 *F FBti0041632 PBac{RB}CG32249e02744 viable and fertile *F FBti0041642 PBac{RB}CG32264e02835 viable and fertile *F FBti0041713 PBac{RB}CG32280e03473 viable and fertile *F FBti0041645 PBac{RB}CG32333e02844 viable and fertile *F FBti0041409 PBac{RB}CG32373e00572 viable and fertile *F FBti0041682 PBac{RB}CG32410e03176 viable and fertile *F FBti0041691 PBac{RB}CG32418e03237 *F FBti0041373 PBac{RB}CG32448e00249 viable and fertile *F FBti0041781 PBac{RB}CG32459e04058 viable and fertile *F FBti0041413 PBac{RB}CG32499e00591 viable and fertile *F FBti0041446 PBac{RB}CG32533e00904 viable and fertile *F FBti0041593 PBac{RB}CG32579e02325 viable and fertile *F FBti0041763 PBac{RB}CG32625e03897 viable and fertile *F FBti0041571 PBac{RB}CG3267e02164 *F FBti0041764 PBac{RB}CG32698e03908 viable and fertile *F FBti0041844 PBac{RB}CG32850e04618 viable and fertile *F FBti0041377 PBac{RB}CG32919e00276 viable and fertile *F FBti0041771 PBac{RB}CG32972e03947 viable and fertile *F FBti0041692 PBac{RB}CG3301e03242 viable and fertile *F FBti0041678 PBac{RB}CG33100e03164 viable and fertile *F FBti0041617 PBac{RB}CG33116e02587 *F FBti0041363 PBac{RB}CG33130e00176 *F FBti0041399 PBac{RB}CG33146e00415 viable and fertile *F FBti0041428 PBac{RB}CG33147e00779 viable and fertile *F FBti0041477 PBac{RB}CG33154e01191 viable and fertile *F FBti0041376 PBac{RB}CG3428e00275 viable and fertile *F FBti0041706 PBac{RB}CG3508e03375 viable and fertile *F FBti0041794 PBac{RB}CG3565e04197 viable and fertile *F FBti0041664 PBac{RB}CG3605e03045 *F FBti0041574 PBac{RB}CG3609e02184 viable and fertile *F FBti0041588 PBac{RB}CG3609e02299 *F FBti0041730 PBac{RB}CG3630e03606 viable and fertile *F FBti0041809 PBac{RB}CG3632e04331 viable and fertile *F FBti0041847 PBac{RB}CG3662e04640 viable and fertile *F FBti0041732 PBac{RB}CG3706e03617 viable and fertile *F FBti0041372 PBac{RB}CG3744e00239 viable and fertile *F FBti0041689 PBac{RB}CG3744e03217 *F FBti0041656 PBac{RB}CG3790e02978 viable and fertile *F FBti0041391 PBac{RB}CG3808e00337 viable and fertile *F FBti0041398 PBac{RB}CG3811e00405 viable and fertile *F FBti0041699 PBac{RB}CG3812e03305 viable and fertile *F FBti0041623 PBac{RB}CG3825e02638 viable and fertile *F FBti0041667 PBac{RB}CG3907e03063 viable and fertile *F FBti0041741 PBac{RB}CG3987e03703 viable and fertile *F FBti0041631 PBac{RB}CG4000e02742 viable and fertile *F FBti0041402 PBac{RB}CG4064e00457 viable and fertile *F FBti0041816 PBac{RB}CG4199e04396 viable and fertile *F FBti0041591 PBac{RB}CG4351e02317 viable and fertile *F FBti0041395 PBac{RB}CG4662e00391 viable and fertile *F FBti0041482 PBac{RB}CG4730e01279 viable and fertile *F FBti0041463 PBac{RB}CG4774e01021 *F FBti0041643 PBac{RB}CG4848e02840 *F FBti0041416 PBac{RB}CG4857e00614 viable and fertile *F FBti0041712 PBac{RB}CG4917e03461 viable and fertile *F FBti0041663 PBac{RB}CG4927e03031 viable and fertile *F FBti0041360 PBac{RB}CG4989e00158 viable and fertile *F FBti0041708 PBac{RB}CG5147e03412 *F FBti0041776 PBac{RB}CG5149e03983 viable and fertile *F FBti0041475 PBac{RB}CG5189e01140 *F FBti0041494 PBac{RB}CG5191e01351 viable and fertile *F FBti0041431 PBac{RB}CG5197e00808 viable and fertile *F FBti0041489 PBac{RB}CG5225e01313 viable and fertile *F FBti0041415 PBac{RB}CG5273e00608 viable and fertile *F FBti0041784 PBac{RB}CG5280e04096 viable and fertile *F FBti0041347 PBac{RB}CG5342e00014 viable and fertile *F FBti0041556 PBac{RB}CG5344e01976 *F FBti0041773 PBac{RB}CG5359e03976 viable and fertile *F FBti0041539 PBac{RB}CG5389e01800 viable and fertile *F FBti0041414 PBac{RB}CG5445e00593 viable and fertile *F FBti0041723 PBac{RB}CG5451e03563 *F FBti0041630 PBac{RB}CG5626e02731 *F FBti0041382 PBac{RB}CG5632e00298 viable and fertile *F FBti0041715 PBac{RB}CG5645e03479 *F FBti0041512 PBac{RB}CG5758e01537 *F FBti0041582 PBac{RB}CG5888e02257 *F FBti0041577 PBac{RB}CG5924e02224 viable and fertile *F FBti0041681 PBac{RB}CG5931e03171 *F FBti0041387 PBac{RB}CG5964e00319 *F FBti0041432 PBac{RB}CG5972e00819 *F FBti0041465 PBac{RB}CG5987e01040 viable and fertile *F FBti0041603 PBac{RB}CG6121e02395 viable and fertile *F FBti0041646 PBac{RB}CG6131e02854 viable and fertile *F FBti0041641 PBac{RB}CG6136e02834 *F FBti0041537 PBac{RB}CG6289e01796 viable and fertile *F FBti0041564 PBac{RB}CG6305e02095 *F FBti0041405 PBac{RB}CG6347e00490 viable and fertile *F FBti0041529 PBac{RB}CG6428e01722 viable and fertile *F FBti0041438 PBac{RB}CG6465e00850 viable and fertile *F FBti0041380 PBac{RB}CG6574e00293 viable and fertile *F FBti0041522 PBac{RB}CG6579e01628 viable and fertile *F FBti0041419 PBac{RB}CG6632e00652 viable and fertile *F FBti0041819 PBac{RB}CG6687e04451 viable and fertile *F FBti0041635 PBac{RB}CG6709e02786 viable and fertile *F FBti0041386 PBac{RB}CG6719e00315 viable and fertile *F FBti0041569 PBac{RB}CG6724e02149 *F FBti0041625 PBac{RB}CG6750e02662 *F FBti0041718 PBac{RB}CG6764e03505 *F FBti0041381 PBac{RB}CG6765e00295 viable and fertile *F FBti0041673 PBac{RB}CG6830e03137 viable and fertile *F FBti0041464 PBac{RB}CG6856e01028 *F FBti0041493 PBac{RB}CG6885e01335 viable and fertile *F FBti0041738 PBac{RB}CG6921e03675 viable and fertile *F FBti0041444 PBac{RB}CG6938e00899 viable and fertile *F FBti0041383 PBac{RB}CG6950e00299 viable and fertile *F FBti0041447 PBac{RB}CG6951e00910 *F FBti0041558 PBac{RB}CG7009e02001 viable and fertile *F FBti0041490 PBac{RB}CG7056e01316 viable and fertile *F FBti0041817 PBac{RB}CG7192e04401 viable and fertile *F FBti0041510 PBac{RB}CG7248e01503 viable and fertile *F FBti0041459 PBac{RB}CG7255e00992 viable and fertile *F FBti0041804 PBac{RB}CG7263e04281 *F FBti0041412 PBac{RB}CG7322e00584 viable and fertile *F FBti0041640 PBac{RB}CG7376e02832 viable and fertile *F FBti0041649 PBac{RB}CG7461e02888 viable and fertile *F FBti0041549 PBac{RB}CG7532e01905 *F FBti0041826 PBac{RB}CG7638e04494 *F FBti0041651 PBac{RB}CG7639e02922 *F FBti0041827 PBac{RB}CG7670e04496 viable and fertile *F FBti0041441 PBac{RB}CG7830e00875 viable and fertile *F FBti0041557 PBac{RB}CG7857e01981 viable and fertile *F FBti0041803 PBac{RB}CG7870e04276 *F FBti0041390 PBac{RB}CG7891e00336 *F FBti0041584 PBac{RB}CG7950e02275 *F FBti0041599 PBac{RB}CG7990e02371 viable and fertile *F FBti0041811 PBac{RB}CG7992e04360 viable and fertile *F FBti0041837 PBac{RB}CG8142e04583 viable and fertile *F FBti0041616 PBac{RB}CG8197e02585 viable and fertile *F FBti0041519 PBac{RB}CG8332e01611 *F FBti0041790 PBac{RB}CG8419e04179 *F FBti0041735 PBac{RB}CG8465e03639 viable and fertile *F FBti0041789 PBac{RB}CG8485e04168 *F FBti0041440 PBac{RB}CG8713e00867 viable and fertile *F FBti0041458 PBac{RB}CG8745e00991 *F FBti0041396 PBac{RB}CG8860e00396 viable and fertile *F FBti0041686 PBac{RB}CG8870e03199 *F FBti0041445 PBac{RB}CG8931e00903 viable and fertile *F FBti0041758 PBac{RB}CG9053e03849 viable and fertile *F FBti0041355 PBac{RB}CG9134e00088 viable and fertile *F FBti0041424 PBac{RB}CG9143e00691 *F FBti0041560 PBac{RB}CG9252e02039 *F FBti0041567 PBac{RB}CG9289e02128 viable and fertile *F FBti0041517 PBac{RB}CG9497e01580 viable and fertile *F FBti0041579 PBac{RB}CG9536e02233 viable and fertile *F FBti0041516 PBac{RB}CG9596e01579 viable and fertile *F FBti0041688 PBac{RB}CG9603e03209 *F FBti0041389 PBac{RB}CG9628e00334 viable and fertile *F FBti0041719 PBac{RB}CG9636e03518 viable and fertile *F FBti0041671 PBac{RB}CG9706e03112 viable and fertile *F FBti0041401 PBac{RB}CG9778e00450 *F FBti0041356 PBac{RB}CG9793e00091 viable and fertile *F FBti0041710 PBac{RB}CG9972e03444 viable and fertile *F FBti0041480 PBac{RB}CG9996e01256 viable and fertile *F FBti0041711 PBac{RB}CG9996e03446 viable and fertile *F FBti0041573 PBac{RB}CSN7e02176 *F FBti0041684 PBac{RB}Cad89De03186 viable and fertile *F FBti0041486 PBac{RB}Cate01301 viable and fertile *F FBti0041767 PBac{RB}Chce03916 viable and fertile *F FBti0041680 PBac{RB}CstF-50e03170 viable and fertile *F FBti0041499 PBac{RB}Cyp313a2e01417 viable and fertile *F FBti0041536 PBac{RB}Cyp9f2e01795 viable and fertile *F FBti0041543 PBac{RB}Dhc62Be01854 viable and fertile *F FBti0041660 PBac{RB}Eaat2e03003 *F FBti0041722 PBac{RB}Ect4e03540 *F FBti0041628 PBac{RB}Edg84Ae02715 *F FBti0041452 PBac{RB}Ef1gammae00972 *F FBti0041472 PBac{RB}Elongin-Ce01107 *F FBti0041520 PBac{RB}Enoe01615 *F FBti0041364 PBac{RB}Fatpe00177 *F FBti0041798 PBac{RB}Fcp3Ce04212 viable and fertile *F FBti0041693 PBac{RB}Fer2e03248 viable and fertile *F FBti0041647 PBac{RB}Fibpe02868 viable and fertile *F FBti0041602 PBac{RB}FucT6e02394 viable and fertile *F FBti0041436 PBac{RB}Ggamma30Ae00834 viable and fertile *F FBti0041814 PBac{RB}GlcAT-Ie04384 viable and fertile *F FBti0041420 PBac{RB}GstE1e00657 viable and fertile *F FBti0041442 PBac{RB}GstE3e00878 *F FBti0041471 PBac{RB}GstE7e01100 viable and fertile *F FBti0041670 PBac{RB}Gyc76Ce03087 viable and fertile *F FBti0041586 PBac{RB}Hcfe02291 viable and fertile *F FBti0041345 PBac{RB}Hexo1e00001 viable and fertile *F FBti0041581 PBac{RB}Hnf4e02246 *F FBti0041727 PBac{RB}Hr78e03593 viable and fertile *F FBti0041518 PBac{RB}Ihe01599 viable and fertile *F FBti0041500 PBac{RB}Kai-RIAe01443 *F FBti0041568 PBac{RB}Khce02141 *F FBti0041504 PBac{RB}Mkk4e01485 *F FBti0041648 PBac{RB}NaPi-Te02874 viable and fertile *F FBti0041397 PBac{RB}Nckx30Ce00401 viable and fertile *F FBti0041538 PBac{RB}Nplp3e01799 viable and fertile *F FBti0041524 PBac{RB}O-fut2e01659 viable and fertile *F FBti0041726 PBac{RB}ORMDLe03591 viable and fertile *F FBti0041455 PBac{RB}Obp83efe00978 viable and fertile *F FBti0041570 PBac{RB}Or49ae02161 viable and fertile *F FBti0041551 PBac{RB}PGRP-LAe01930 viable and fertile *F FBti0041629 PBac{RB}Pbprp4e02730 viable and fertile *F FBti0041575 PBac{RB}Pde11e02198 viable and fertile *F FBti0041753 PBac{RB}Pde11e03811 *F FBti0041772 PBac{RB}Pi3K59Fe03961 viable and fertile *F FBti0041598 PBac{RB}Picote02367 *F FBti0041818 PBac{RB}Pkcdeltae04408 viable and fertile *F FBti0041760 PBac{RB}REGe03858 viable and fertile *F FBti0041483 PBac{RB}Rab1e01287 *F FBti0041845 PBac{RB}Rapgap1e04634 viable and fertile *F FBti0041733 PBac{RB}Rbp1-likee03624 viable and fertile *F FBti0041822 PBac{RB}Rbp1e04474 viable and fertile *F FBti0041621 PBac{RB}Rca1e02614 viable and fertile *F FBti0041820 PBac{RB}Rh4e04456 viable and fertile *F FBti0041748 PBac{RB}RhoGEF2e03784 *F FBti0041786 PBac{RB}RpL23Ae04143 viable and fertile *F FBti0041594 PBac{RB}RpL7Ae02336 viable and fertile *F FBti0041654 PBac{RB}Samuele02949 *F FBti0041484 PBac{RB}Sap-re01294 viable and fertile *F FBti0041746 PBac{RB}Ski6e03781 viable and fertile *F FBti0041583 PBac{RB}Ssb-c31ae02272 viable and fertile *F FBti0041423 PBac{RB}Stame00677 viable and fertile *F FBti0041637 PBac{RB}Sur-8e02803 *F FBti0041426 PBac{RB}Sure00744 *F FBti0041371 PBac{RB}Syne00238 viable and fertile *F FBti0041759 PBac{RB}Ten-ae03855 viable and fertile *F FBti0041496 PBac{RB}TfIIEalphae01382 *F FBti0041393 PBac{RB}TfIIEbetae00364 viable and fertile *F FBti0041696 PBac{RB}Tsp3Ae03287 viable and fertile *F FBti0041421 PBac{RB}Tsp42Eke00673 viable and fertile *F FBti0041668 PBac{RB}Tsp42Eqe03064 viable and fertile *F FBti0041778 PBac{RB}Ucp4Ce03988 viable and fertile *F FBti0041439 PBac{RB}Ugt86Die00862 *F FBti0041807 PBac{RB}VhaAC39e04316 viable and fertile *F FBti0041702 PBac{RB}X11Le03317 viable and fertile *F FBti0041701 PBac{RB}Yp2e03312 viable and fertile *F FBti0041839 PBac{RB}arme04595 *F FBti0041353 PBac{RB}arre00083 *F FBti0041675 PBac{RB}atte03144 *F FBti0041435 PBac{RB}bchse00833 *F FBti0041434 PBac{RB}beat-Ibe00831 viable and fertile *F FBti0041540 PBac{RB}betaTub97EFe01802 viable and fertile *F FBti0041562 PBac{RB}bwae02081 viable and fertile *F FBti0041752 PBac{RB}cypee03803 *F FBti0041503 PBac{RB}deie01478 viable and fertile *F FBti0041550 PBac{RB}dibe01927 viable and fertile *F FBti0041433 PBac{RB}dreame00821 *F FBti0041697 PBac{RB}e(y)2e03298 viable and fertile *F FBti0041788 PBac{RB}empe04154 *F FBti0041578 PBac{RB}frede02229 *F FBti0041734 PBac{RB}fs(1)Yae03625 viable and fertile *F FBti0041533 PBac{RB}gishe01759 *F FBti0041354 PBac{RB}grpe00087 viable and fertile *F FBti0041429 PBac{RB}inaCe00783 viable and fertile *F FBti0041762 PBac{RB}kirree03862 viable and fertile *F FBti0041687 PBac{RB}kkve03205 *F FBti0041838 PBac{RB}l(1)10Bbe04588 *F FBti0041501 PBac{RB}mRpL20e01458 viable and fertile *F FBti0041580 PBac{RB}mRpS35e02239 *F FBti0041768 PBac{RB}mewe03932 viable and fertile *F FBti0041676 PBac{RB}miplee03148 viable and fertile *F FBti0041850 PBac{RB}mlee04702 viable and fertile *F FBti0041626 PBac{RB}mole02670 *F FBti0041495 PBac{RB}mthl2e01373 viable and fertile *F FBti0041815 PBac{RB}nae04385 viable and fertile *F FBti0041385 PBac{RB}ninaGe00313 viable and fertile *F FBti0041724 PBac{RB}nufe03568 viable and fertile *F FBti0041357 PBac{RB}onecute00113 viable and fertile *F FBti0041563 PBac{RB}orte02083 viable and fertile *F FBti0041653 PBac{RB}pxe02948 viable and fertile *F FBti0041561 PBac{RB}qkr54Be02070 *F FBti0041532 PBac{RB}r-le01755 viable and fertile *F FBti0041829 PBac{RB}rbe04526 *F FBti0041394 PBac{RB}rhae00367 viable and fertile *F FBti0041411 PBac{RB}ruxe00579 viable and fertile *F FBti0041346 PBac{RB}scpr-Be00007 viable and fertile *F FBti0041677 PBac{RB}sloe03162 viable and fertile *F FBti0041707 PBac{RB}snke03379 viable and fertile *F FBti0041634 PBac{RB}spoe02785 viable and fertile *F FBti0041782 PBac{RB}su(Hw)e04061 *F FBti0041620 PBac{RB}synaptojanine02597 *F FBti0041359 PBac{RB}tore00150 viable and fertile *F FBti0041534 PBac{RB}trae01760 viable and fertile *F FBti0041694 PBac{RB}tube03259 viable and fertile *F FBti0041418 PBac{RB}wgne00637 viable and fertile *F FBti0041461 PBac{RB}yellow-e3e01012 *F FBti0041348 PBac{RB}zetaCOPe00020 *F FBti0042654 PBac{WH}140upf07279 *F FBti0042434 PBac{WH}5-HT7f05214 viable and fertile *F FBti0042199 PBac{WH}AP-2f03132 *F FBti0041886 PBac{WH}Act79Bf00364 viable and fertile *F FBti0041964 PBac{WH}Actn3f00971 *F FBti0042198 PBac{WH}AlCR2f03116 viable and fertile *F FBti0042319 PBac{WH}Arc105f04180 *F FBti0042308 PBac{WH}Arp14Df04069 *F FBti0042006 PBac{WH}B4f01442 viable and fertile *F FBti0042491 PBac{WH}Bap60f05782 viable and fertile *F FBti0042566 PBac{WH}Bteb2f06447 viable and fertile *F FBti0042227 PBac{WH}Bub3f03381 *F FBti0042327 PBac{WH}Bx42f04222 viable and fertile *F FBti0042583 PBac{WH}CG10038f06638 viable and fertile *F FBti0042600 PBac{WH}CG10039f06819 viable and fertile *F FBti0042266 PBac{WH}CG10064f03756 *F FBti0042416 PBac{WH}CG10083f05024 viable and fertile *F FBti0042366 PBac{WH}CG10137f04546 *F FBti0042195 PBac{WH}CG10168f03105 *F FBti0042588 PBac{WH}CG10195f06699 viable and fertile *F FBti0042183 PBac{WH}CG10217f03018 *F FBti0042078 PBac{WH}CG10253f02060 *F FBti0042132 PBac{WH}CG10289f02530 *F FBti0042599 PBac{WH}CG10326f06814 viable and fertile *F FBti0041977 PBac{WH}CG10335f01110 viable and fertile *F FBti0042698 PBac{WH}CG10341f07749 *F FBti0042292 PBac{WH}CG10376f03911 viable and fertile *F FBti0042343 PBac{WH}CG10384f04349 viable and fertile *F FBti0042241 PBac{WH}CG10435f03495 viable and fertile *F FBti0042270 PBac{WH}CG10486f03784 viable and fertile *F FBti0041880 PBac{WH}CG10508f00325 viable and fertile *F FBti0041905 PBac{WH}CG10560f00512 viable and fertile *F FBti0042541 PBac{WH}CG10561f06260 viable and fertile *F FBti0042321 PBac{WH}CG10602f04195 *F FBti0041936 PBac{WH}CG10617f00785 viable and fertile *F FBti0041980 PBac{WH}CG10632f01127 viable and fertile *F FBti0041881 PBac{WH}CG10638f00334 viable and fertile *F FBti0042098 PBac{WH}CG10639f02237 viable and fertile *F FBti0042442 PBac{WH}CG10674f05385 viable and fertile *F FBti0042286 PBac{WH}CG10702f03893 viable and fertile *F FBti0042237 PBac{WH}CG10703f03477 viable and fertile *F FBti0042203 PBac{WH}CG10754f03161 *F FBti0042087 PBac{WH}CG10806f02140 viable and fertile *F FBti0042023 PBac{WH}CG10809f01651 viable and fertile *F FBti0042273 PBac{WH}CG10855f03797 viable and fertile *F FBti0042652 PBac{WH}CG10855f07259 viable and fertile *F FBti0042451 PBac{WH}CG10866f05447 viable and fertile *F FBti0042606 PBac{WH}CG10882f06847 viable and fertile *F FBti0042256 PBac{WH}CG10916f03629 viable and fertile *F FBti0041910 PBac{WH}CG10919f00574 viable and fertile *F FBti0042359 PBac{WH}CG10932f04498 viable and fertile *F FBti0042444 PBac{WH}CG10984f05402 viable and fertile *F FBti0042007 PBac{WH}CG11044f01462 viable and fertile *F FBti0041862 PBac{WH}CG11048f00140 viable and fertile *F FBti0041933 PBac{WH}CG11077f00774 viable and fertile *F FBti0042166 PBac{WH}CG11120f02851 viable and fertile *F FBti0042397 PBac{WH}CG11141f04868 viable and fertile *F FBti0042300 PBac{WH}CG11164f03984 viable and fertile *F FBti0041988 PBac{WH}CG11206f01268 viable and fertile *F FBti0042156 PBac{WH}CG11211f02744 viable and fertile *F FBti0042013 PBac{WH}CG11237f01510 *F FBti0041902 PBac{WH}CG11247f00485 viable and fertile *F FBti0042549 PBac{WH}CG11257f06315 viable and fertile *F FBti0042542 PBac{WH}CG11319f06271 viable and fertile *F FBti0042658 PBac{WH}CG11386f07325a viable and fertile *F FBti0042473 PBac{WH}CG11406f05598 viable and fertile *F FBti0041883 PBac{WH}CG1140f00344 viable and fertile *F FBti0042632 PBac{WH}CG11505f07116 viable and fertile *F FBti0042679 PBac{WH}CG1151f07607 *F FBti0042448 PBac{WH}CG11523f05435 viable and fertile *F FBti0041989 PBac{WH}CG11526f01271 viable and fertile *F FBti0041863 PBac{WH}CG11598f00150 viable and fertile *F FBti0041941 PBac{WH}CG11641f00828 *F FBti0042173 PBac{WH}CG11655f02934 viable and fertile *F FBti0042301 PBac{WH}CG11710f03997 viable and fertile *F FBti0042004 PBac{WH}CG11750f01412 viable and fertile *F FBti0042143 PBac{WH}CG11836f02631 *F FBti0041927 PBac{WH}CG11848f00718 viable and fertile *F FBti0042648 PBac{WH}CG11874f07221 viable and fertile *F FBti0041952 PBac{WH}CG11906f00885 viable and fertile *F FBti0041882 PBac{WH}CG11951f00339 viable and fertile *F FBti0042581 PBac{WH}CG12063f06602 *F FBti0042553 PBac{WH}CG12112f06346 viable and fertile *F FBti0042377 PBac{WH}CG12171f04657 viable and fertile *F FBti0042659 PBac{WH}CG12206f07347 viable and fertile *F FBti0042515 PBac{WH}CG12267f05999 *F FBti0041993 PBac{WH}CG12314f01342 viable and fertile *F FBti0042043 PBac{WH}CG12428f01800 viable and fertile *F FBti0042185 PBac{WH}CG12487f03024 viable and fertile *F FBti0042260 PBac{WH}CG12565f03677 viable and fertile *F FBti0042704 PBac{WH}CG12612f07796 viable and fertile *F FBti0042708 PBac{WH}CG12659f07899 *F FBti0041995 PBac{WH}CG12714f01354 viable and fertile *F FBti0041960 PBac{WH}CG12736f00932 viable and fertile *F FBti0042685 PBac{WH}CG12753f07675 *F FBti0042354 PBac{WH}CG12772f04462 viable and fertile *F FBti0042254 PBac{WH}CG12820f03609 viable and fertile *F FBti0042071 PBac{WH}CG12822f02022 *F FBti0041875 PBac{WH}CG12870f00299 viable and fertile *F FBti0042130 PBac{WH}CG12885f02499 viable and fertile *F FBti0042623 PBac{WH}CG12901f07056 *F FBti0042249 PBac{WH}CG12904f03574 *F FBti0042582 PBac{WH}CG12938f06616 viable and fertile *F FBti0042486 PBac{WH}CG33287f05728 viable and fertile *F FBti0042594 PBac{WH}CG12984f06763 viable and fertile *F FBti0042376 PBac{WH}CG13036f04649 viable and fertile *F FBti0042165 PBac{WH}CG13073f02831 viable and fertile *F FBti0042539 PBac{WH}CG13082f06243 viable and fertile *F FBti0042188 PBac{WH}CG1308f03050 viable and fertile *F FBti0042340 PBac{WH}CG13131f04310 viable and fertile *F FBti0041860 PBac{WH}CG13141f00117 viable and fertile *F FBti0042220 PBac{WH}CG13155f03327 viable and fertile *F FBti0042228 PBac{WH}CG1315f03391 viable and fertile *F FBti0042579 PBac{WH}CG13162f06590 viable and fertile *F FBti0042694 PBac{WH}CG13325f07727 viable and fertile *F FBti0042119 PBac{WH}CG13358f02375 viable and fertile *F FBti0042326 PBac{WH}CG1338f04221 *F FBti0042272 PBac{WH}CG13458f03792 viable and fertile *F FBti0042147 PBac{WH}CG13466f02655 *F FBti0042369 PBac{WH}CG13527f04582 *F FBti0042344 PBac{WH}CG13538f04366 viable and fertile *F FBti0042096 PBac{WH}CG13563f02213 viable and fertile *F FBti0042603 PBac{WH}CG13568f06838 viable and fertile *F FBti0041893 PBac{WH}CG13579f00432 viable and fertile *F FBti0041959 PBac{WH}CG13594f00918 *F FBti0042141 PBac{WH}CG13602f02622 viable and fertile *F FBti0042474 PBac{WH}CG13688f05607 *F FBti0042646 PBac{WH}CG13738f07206 viable and fertile *F FBti0042012 PBac{WH}CG13793f01503 viable and fertile *F FBti0042629 PBac{WH}CG13796f07097a viable and fertile *F FBti0041973 PBac{WH}CG13926f01052 viable and fertile *F FBti0042322 PBac{WH}CG13946f04199 viable and fertile *F FBti0042691 PBac{WH}CG14007f07713 viable and fertile *F FBti0042619 PBac{WH}CG1407f07024 viable and fertile *F FBti0041919 PBac{WH}CG14101f00638 viable and fertile *F FBti0042034 PBac{WH}CG14103f01739 viable and fertile *F FBti0042678 PBac{WH}CG14104f07593 viable and fertile *F FBti0042484 PBac{WH}CG14133f05716 *F FBti0042537 PBac{WH}CG1420f06221 *F FBti0042355 PBac{WH}CG14210f04478 viable and fertile *F FBti0042662 PBac{WH}CG14229f07368 viable and fertile *F FBti0042306 PBac{WH}CG14235f04041 *F FBti0042413 PBac{WH}CG14292f04985 viable and fertile *F FBti0042592 PBac{WH}CG14318f06747 viable and fertile *F FBti0042233 PBac{WH}CG14325f03448 viable and fertile *F FBti0042033 PBac{WH}CG14365f01731 viable and fertile *F FBti0042313 PBac{WH}CG1441f04134 viable and fertile *F FBti0042571 PBac{WH}CG14439f06522 viable and fertile *F FBti0042561 PBac{WH}CG14442f06399 viable and fertile *F FBti0042401 PBac{WH}CG14463f04901 viable and fertile *F FBti0042403 PBac{WH}CG14509f04904 viable and fertile *F FBti0042497 PBac{WH}CG14565f05859 viable and fertile *F FBti0042527 PBac{WH}CG14618f06141 viable and fertile *F FBti0042178 PBac{WH}CG14634f02973 viable and fertile *F FBti0042196 PBac{WH}CG14693f03110 viable and fertile *F FBti0042433 PBac{WH}CG14741f05203 viable and fertile *F FBti0042399 PBac{WH}CG14756f04886 viable and fertile *F FBti0041873 PBac{WH}CG14795f00285 viable and fertile *F FBti0042408 PBac{WH}CG14846f04940 *F FBti0042407 PBac{WH}CG14894f04937 *F FBti0042653 PBac{WH}CG14990f07267 viable and fertile *F FBti0042634 PBac{WH}CG14998f07121 viable and fertile *F FBti0042302 PBac{WH}CG1501f04004 viable and fertile *F FBti0042718 PBac{WH}CG15029f08019 viable and fertile *F FBti0042425 PBac{WH}CG15104f05115 *F FBti0042155 PBac{WH}CG15105f02741 viable and fertile *F FBti0041901 PBac{WH}CG15119f00474 *F FBti0042573 PBac{WH}CG15170f06529 *F FBti0042429 PBac{WH}CG15173f05160 viable and fertile *F FBti0042274 PBac{WH}CG15177f03800 viable and fertile *F FBti0042138 PBac{WH}CG15184f02608 viable and fertile *F FBti0042621 PBac{WH}CG15235f07038 viable and fertile *F FBti0042382 PBac{WH}CG15256f04709 viable and fertile *F FBti0042110 PBac{WH}CG15270f02323 viable and fertile *F FBti0042115 PBac{WH}CG15304f02352 viable and fertile *F FBti0041872 PBac{WH}CG15308f00264 viable and fertile *F FBti0042492 PBac{WH}CG15332f05798 viable and fertile *F FBti0041999 PBac{WH}CG15370f01385 viable and fertile *F FBti0042480 PBac{WH}CG15406f05661 viable and fertile *F FBti0042546 PBac{WH}CG15436f06292 viable and fertile *F FBti0042701 PBac{WH}CG15436f07761 viable and fertile *F FBti0042590 PBac{WH}CG1544f06723 viable and fertile *F FBti0042557 PBac{WH}CG15452f06385 viable and fertile *F FBti0042660 PBac{WH}CG15478f07358 viable and fertile *F FBti0042432 PBac{WH}CG15538f05196 *F FBti0042349 PBac{WH}CG15616f04406 viable and fertile *F FBti0042250 PBac{WH}CG15626f03581 *F FBti0042724 PBac{WH}CG15635f08079 viable and fertile *F FBti0042608 PBac{WH}CG15706f06892 *F FBti0042577 PBac{WH}CG15707f06583 *F FBti0042298 PBac{WH}CG15721f03962 viable and fertile *F FBti0042488 PBac{WH}CG15731f05765 viable and fertile *F FBti0042171 PBac{WH}CG15765f02896 viable and fertile *F FBti0042361 PBac{WH}CG15772f04506 viable and fertile *F FBti0042117 PBac{WH}CG15780f02362 viable and fertile *F FBti0042172 PBac{WH}CG15782f02921 viable and fertile *F FBti0042281 PBac{WH}CG15874f03854 viable and fertile *F FBti0041870 PBac{WH}CG15894f00252 viable and fertile *F FBti0042371 PBac{WH}CG15905f04600 viable and fertile *F FBti0042428 PBac{WH}CG1602f05157 viable and fertile *F FBti0042386 PBac{WH}CG1603f04743 *F FBti0041916 PBac{WH}CG16762f00614 viable and fertile *F FBti0042559 PBac{WH}CG16782f06392 viable and fertile *F FBti0042562 PBac{WH}CG1681f06400 viable and fertile *F FBti0042210 PBac{WH}CG16886f03248 viable and fertile *F FBti0042597 PBac{WH}CG16986f06804 viable and fertile *F FBti0042687 PBac{WH}CG17064f07689 viable and fertile *F FBti0042702 PBac{WH}CG17118f07762 viable and fertile *F FBti0042280 PBac{WH}CG17141f03838 *F FBti0042245 PBac{WH}CG17150f03522 viable and fertile *F FBti0042593 PBac{WH}CG17181f06749 viable and fertile *F FBti0042187 PBac{WH}CG17186f03032 viable and fertile *F FBti0042513 PBac{WH}CG17379f05980 *F FBti0042144 PBac{WH}CG17562f02635 *F FBti0042520 PBac{WH}CG17600f06072 viable and fertile *F FBti0042680 PBac{WH}CG17625f07631 viable and fertile *F FBti0042239 PBac{WH}CG17639f03487 viable and fertile *F FBti0042714 PBac{WH}CG1764f07996 viable and fertile *F FBti0042456 PBac{WH}CG17712f05481 viable and fertile *F FBti0042420 PBac{WH}CG17746f05041 viable and fertile *F FBti0042014 PBac{WH}CG1776f01512 *F FBti0042615 PBac{WH}CG17917f06982 viable and fertile *F FBti0042531 PBac{WH}CG1792f06161 viable and fertile *F FBti0042338 PBac{WH}CG18131f04301 viable and fertile *F FBti0042238 PBac{WH}CG18208f03483 viable and fertile *F FBti0042184 PBac{WH}CG18231f03022 viable and fertile *F FBti0042259 PBac{WH}CG18273f03674 viable and fertile *F FBti0042008 PBac{WH}CG18327f01465 viable and fertile *F FBti0042493 PBac{WH}CG18358f05802 viable and fertile *F FBti0042305 PBac{WH}CG1839f04022 viable and fertile *F FBti0042145 PBac{WH}CG18496f02641 viable and fertile *F FBti0042086 PBac{WH}CG18539f02127 *F FBti0042213 PBac{WH}CG18604f03280 viable and fertile *F FBti0042201 PBac{WH}CG18675f03146 viable and fertile *F FBti0042640 PBac{WH}CG1887f07156 *F FBti0042534 PBac{WH}CG1894f06204 *F FBti0041976 PBac{WH}CG1919f01099 *F FBti0042287 PBac{WH}CG1942f03896 viable and fertile *F FBti0042391 PBac{WH}CG1962f04787 viable and fertile *F FBti0042045 PBac{WH}CG2046f01807 viable and fertile *F FBti0042148 PBac{WH}CG2107f02667 *F FBti0042713 PBac{WH}CG2111f07993 viable and fertile *F FBti0041946 PBac{WH}CG2121f00867 *F FBti0042611 PBac{WH}CG2199f06926 viable and fertile *F FBti0041943 PBac{WH}CG2316f00836 viable and fertile *F FBti0042572 PBac{WH}CG2555f06525 viable and fertile *F FBti0041871 PBac{WH}CG2652f00262 viable and fertile *F FBti0042554 PBac{WH}CG2658f06350 viable and fertile *F FBti0042721 PBac{WH}CG2713f08040 viable and fertile *F FBti0042000 PBac{WH}CG2750f01388 viable and fertile *F FBti0042258 PBac{WH}CG2967f03664 viable and fertile *F FBti0042358 PBac{WH}CG2968f04497 viable and fertile *F FBti0042100 PBac{WH}CG2970f02243 viable and fertile *F FBti0042114 PBac{WH}CG2974f02346 viable and fertile *F FBti0042015 PBac{WH}CG30010f01531 *F FBti0042009 PBac{WH}CG30015f01474 viable and fertile *F FBti0042127 PBac{WH}CG30016f02466 viable and fertile *F FBti0042089 PBac{WH}CG30020f02147 viable and fertile *F FBti0042555 PBac{WH}CG30090f06357 viable and fertile *F FBti0042460 PBac{WH}CG30096f05490 viable and fertile *F FBti0042471 PBac{WH}CG30101f05583 viable and fertile *F FBti0042122 PBac{WH}CG30109f02403 viable and fertile *F FBti0042329 PBac{WH}CG30127f04245 viable and fertile *F FBti0042601 PBac{WH}CG30158f06824 *F FBti0042624 PBac{WH}CG30180f07066 viable and fertile *F FBti0042363 PBac{WH}CG30184f04532 viable and fertile *F FBti0041944 PBac{WH}CG30290f00839 *F FBti0042367 PBac{WH}CG30437f04551 *F FBti0042111 PBac{WH}CG3044f02328 viable and fertile *F FBti0042282 PBac{WH}CG30457f03866 viable and fertile *F FBti0042457 PBac{WH}CG30499f05482 viable and fertile *F FBti0042289 PBac{WH}CG3058f03902 viable and fertile *F FBti0042412 PBac{WH}CG31004f04955 *F FBti0042642 PBac{WH}CG31063f07185 viable and fertile *F FBti0042019 PBac{WH}CG31077f01593 viable and fertile *F FBti0041906 PBac{WH}CG31090f00513 viable and fertile *F FBti0042048 PBac{WH}CG31109f01847 viable and fertile *F FBti0042650 PBac{WH}CG31121f07249 *F FBti0042021 PBac{WH}CG31187f01626 viable and fertile *F FBti0042485 PBac{WH}CG31205f05726 viable and fertile *F FBti0042030 PBac{WH}CG31217f01711 viable and fertile *F FBti0042417 PBac{WH}CG31221f05025 viable and fertile *F FBti0042655 PBac{WH}CG31249f07289 viable and fertile *F FBti0042378 PBac{WH}CG31301f04660 viable and fertile *F FBti0042278 PBac{WH}CG31311f03836 viable and fertile *F FBti0042025 PBac{WH}CG31316f01681 *F FBti0042380 PBac{WH}CG31323f04673 viable and fertile *F FBti0042636 PBac{WH}CG31361f07135 viable and fertile *F FBti0042516 PBac{WH}CG31363f06000 viable and fertile *F FBti0042707 PBac{WH}CG31374f07849 *F FBti0042503 PBac{WH}CG31388f05917 viable and fertile *F FBti0042275 PBac{WH}CG31391f03806 viable and fertile *F FBti0042447 PBac{WH}CG31395f05434 viable and fertile *F FBti0042031 PBac{WH}CG31454f01723 viable and fertile *F FBti0042726 PBac{WH}CG31463f08104 viable and fertile *F FBti0041909 PBac{WH}CG31530f00567 viable and fertile *F FBti0042372 PBac{WH}CG31600f04602 viable and fertile *F FBti0042465 PBac{WH}CG31640f05536 viable and fertile *F FBti0042673 PBac{WH}CG31652f07566 viable and fertile *F FBti0042478 PBac{WH}CG31676f05642 viable and fertile *F FBti0042550 PBac{WH}CG31718f06333 viable and fertile *F FBti0042129 PBac{WH}CG31728f02493 *F FBti0042248 PBac{WH}CG31728f03572 viable and fertile *F FBti0042102 PBac{WH}CG31731f02254 viable and fertile *F FBti0042216 PBac{WH}CG31746f03296 viable and fertile *F FBti0042153 PBac{WH}CG31751f02725 viable and fertile *F FBti0042123 PBac{WH}CG31757f02409 viable and fertile *F FBti0041892 PBac{WH}CG31760f00408 viable and fertile *F FBti0041899 PBac{WH}CG31760f00461 viable and fertile *F FBti0042215 PBac{WH}CG31784f03289 viable and fertile *F FBti0042394 PBac{WH}CG31791f04849 viable and fertile *F FBti0042699 PBac{WH}CG31805f07750 *F FBti0042158 PBac{WH}CG31871f02763 viable and fertile *F FBti0041853 PBac{WH}CG31901f00026 viable and fertile *F FBti0041894 PBac{WH}CG31908f00433 *F FBti0041890 PBac{WH}CG31918f00386 *F FBti0042091 PBac{WH}CG31956f02186 *F FBti0042467 PBac{WH}CG31957f05539 *F FBti0042072 PBac{WH}CG31960f02028 *F FBti0053191 PBac{WH}CG31961f06868 viable and fertile *F FBti0042092 PBac{WH}CG31989f02191 *F FBti0042396 PBac{WH}CG31990f04861 viable and fertile *F FBti0042591 PBac{WH}CG32044f06738 viable and fertile *F FBti0042439 PBac{WH}CG32046f05273 viable and fertile *F FBti0042240 PBac{WH}CG32058f03494 viable and fertile *F FBti0042190 PBac{WH}CG32070f03058 viable and fertile *F FBti0042677 PBac{WH}CG32081f07587 viable and fertile *F FBti0042267 PBac{WH}CG32111f03764 *F FBti0042711 PBac{WH}CG32112f07936 viable and fertile *F FBti0042499 PBac{WH}CG32174f05870 *F FBti0042244 PBac{WH}CG32183f03521 viable and fertile *F FBti0042630 PBac{WH}CG32206f07105 viable and fertile *F FBti0042437 PBac{WH}CG32227f05264 *F FBti0041922 PBac{WH}CG32230f00651 viable and fertile *F FBti0041968 PBac{WH}CG32245f01008 viable and fertile *F FBti0041887 PBac{WH}CG32296f00366 viable and fertile *F FBti0042070 PBac{WH}CG32373f02001 *F FBti0042018 PBac{WH}CG32376f01583 viable and fertile *F FBti0042532 PBac{WH}CG32388f06166 *F FBti0042022 PBac{WH}CG32425f01627 viable and fertile *F FBti0042149 PBac{WH}CG32432f02670 viable and fertile *F FBti0042017 PBac{WH}CG32441f01564 viable and fertile *F FBti0041962 PBac{WH}CG32444f00963 viable and fertile *F FBti0041928 PBac{WH}CG32446f00729 viable and fertile *F FBti0042501 PBac{WH}CG32457f05911 viable and fertile *F FBti0041876 PBac{WH}CG32462f00303 viable and fertile *F FBti0041934 PBac{WH}CG32465f00777 *F FBti0041869 PBac{WH}CG32506f00215 viable and fertile *F FBti0042522 PBac{WH}CG32510f06097 viable and fertile *F FBti0042564 PBac{WH}CG32511f06420 viable and fertile *F FBti0042715 PBac{WH}CG32514f08012 viable and fertile *F FBti0042177 PBac{WH}CG32521f02961 viable and fertile *F FBti0042356 PBac{WH}CG32532f04479 viable and fertile *F FBti0042563 PBac{WH}CG32547f06408 viable and fertile *F FBti0042570 PBac{WH}CG9132f06514 viable and fertile *F FBti0041874 PBac{WH}CG32580f00286 viable and fertile *F FBti0042551 PBac{WH}CG32584f06338 viable and fertile *F FBti0042719 PBac{WH}CG32605f08020c viable and fertile *F FBti0042113 PBac{WH}CG32635f02335 viable and fertile *F FBti0042353 PBac{WH}CG32647f04453 viable and fertile *F FBti0042170 PBac{WH}CG32663f02881 viable and fertile *F FBti0042558 PBac{WH}CG32678f06390 viable and fertile *F FBti0042580 PBac{WH}CG32697f06600 viable and fertile *F FBti0042051 PBac{WH}CG3280f01875 viable and fertile *F FBti0042468 PBac{WH}CG32843f05546 viable and fertile *F FBti0042222 PBac{WH}CG3285f03337 viable and fertile *F FBti0042523 PBac{WH}CG32918f06121 viable and fertile *F FBti0042246 PBac{WH}CG32922f03543 viable and fertile *F FBti0042080 PBac{WH}CG3294f02075 viable and fertile *F FBti0042364 PBac{WH}CG33013f04542 viable and fertile *F FBti0042500 PBac{WH}CG33057f05871 viable and fertile *F FBti0042133 PBac{WH}CG33062f02537 viable and fertile *F FBti0042351 PBac{WH}CG33067f04441 viable and fertile *F FBti0042697 PBac{WH}CG33129f07745 viable and fertile *F FBti0042337 PBac{WH}CG33145f04299 viable and fertile *F FBti0042651 PBac{WH}CG33171f07253 viable and fertile *F FBti0042001 PBac{WH}CG33188f01391 *F FBti0042038 PBac{WH}CG33275f01768 viable and fertile *F FBti0042109 PBac{WH}CG3328f02302 viable and fertile *F FBti0041859 PBac{WH}CG33322f00115 viable and fertile *F FBti0042120 PBac{WH}CG3342f02379 viable and fertile *F FBti0042578 PBac{WH}CG3362f06587 viable and fertile *F FBti0041891 PBac{WH}CG3367f00393 viable and fertile *F FBti0042398 PBac{WH}CG3520f04874 viable and fertile *F FBti0041856 PBac{WH}CG3536f00046 viable and fertile *F FBti0042323 PBac{WH}CG3541f04210 viable and fertile *F FBti0041914 PBac{WH}CG3563f00590 *F FBti0042303 PBac{WH}CG3588f04005 viable and fertile *F FBti0042423 PBac{WH}CG3589f05097 viable and fertile *F FBti0042105 PBac{WH}CG3626f02285 viable and fertile *F FBti0041918 PBac{WH}CG3631f00630 viable and fertile *F FBti0042103 PBac{WH}CG3645f02260 *F FBti0042175 PBac{WH}CG3655f02955 viable and fertile *F FBti0042589 PBac{WH}CG3751f06717 viable and fertile *F FBti0042375 PBac{WH}CG3764f04642 *F FBti0042283 PBac{WH}CG3793f03871 viable and fertile *F FBti0042390 PBac{WH}CG3803f04773 *F FBti0042430 PBac{WH}CG3805f05168 viable and fertile *F FBti0042242 PBac{WH}CG3822f03502 viable and fertile *F FBti0042352 PBac{WH}CG3878f04443 viable and fertile *F FBti0042076 PBac{WH}CG3927f02055 viable and fertile *F FBti0042596 PBac{WH}CG4074f06797 viable and fertile *F FBti0042061 PBac{WH}CG4196f01946 viable and fertile *F FBti0042514 PBac{WH}CG4288f05992 viable and fertile *F FBti0042002 PBac{WH}CG4293f01393 viable and fertile *F FBti0042159 PBac{WH}CG4341f02775 viable and fertile *F FBti0042243 PBac{WH}CG4347f03515 *F FBti0041923 PBac{WH}CG4362f00668 viable and fertile *F FBti0042073 PBac{WH}CG4383f02042 viable and fertile *F FBti0041940 PBac{WH}CG4389f00822 *F FBti0042160 PBac{WH}CG4424f02794 viable and fertile *F FBti0042347 PBac{WH}CG4450f04377 viable and fertile *F FBti0042029 PBac{WH}CG4484f01710 *F FBti0042475 PBac{WH}CG4497f05611 viable and fertile *F FBti0041985 PBac{WH}CG4547f01201 viable and fertile *F FBti0041986 PBac{WH}CG4586f01234 viable and fertile *F FBti0042341 PBac{WH}CG4594f04335 viable and fertile *F FBti0041857 PBac{WH}CG4658f00098 viable and fertile *F FBti0042265 PBac{WH}CG4678f03725 viable and fertile *F FBti0042454 PBac{WH}CG4729f05465 viable and fertile *F FBti0042682 PBac{WH}CG4730f07640 viable and fertile *F FBti0042191 PBac{WH}CG4743f03065 viable and fertile *F FBti0041903 PBac{WH}CG4753f00486 viable and fertile *F FBti0042405 PBac{WH}CG4771f04929 viable and fertile *F FBti0042695 PBac{WH}CG4892f07738 viable and fertile *F FBti0042725 PBac{WH}CG4893f08099 viable and fertile *F FBti0041932 PBac{WH}CG4911f00751 *F FBti0042065 PBac{WH}CG4925f01978 *F FBti0042085 PBac{WH}CG4945f02115 viable and fertile *F FBti0042101 PBac{WH}CG4959f02253 viable and fertile *F FBti0042140 PBac{WH}CG5003f02616 *F FBti0042690 PBac{WH}CG5003f07705 viable and fertile *F FBti0042324 PBac{WH}CG5091f04215 *F FBti0041958 PBac{WH}CG5126f00916 viable and fertile *F FBti0042418 PBac{WH}CG5150f05027 viable and fertile *F FBti0042049 PBac{WH}CG5161f01855 viable and fertile *F FBti0041851 PBac{WH}CG5174f00017 viable and fertile *F FBti0042234 PBac{WH}CG5237f03453 viable and fertile *F FBti0042059 PBac{WH}CG5285f01940 viable and fertile *F FBti0042136 PBac{WH}CG5290f02563 *F FBti0042585 PBac{WH}CG5322f06685 viable and fertile *F FBti0041935 PBac{WH}CG5384f00779 viable and fertile *F FBti0042194 PBac{WH}CG5451f03090 *F FBti0042062 PBac{WH}CG5500f01962 viable and fertile *F FBti0042404 PBac{WH}CG5508f04927 *F FBti0042139 PBac{WH}CG5515f02614 *F FBti0042395 PBac{WH}CG5543f04855 *F FBti0042504 PBac{WH}CG5567f05921 *F FBti0042635 PBac{WH}CG5567f07123 viable and fertile *F FBti0042529 PBac{WH}CG5589f06152 *F FBti0041954 PBac{WH}CG5602f00902 *F FBti0042631 PBac{WH}CG5611f07113 viable and fertile *F FBti0042176 PBac{WH}CG5613f02957 viable and fertile *F FBti0042379 PBac{WH}CG5614f04664 viable and fertile *F FBti0042510 PBac{WH}CG5618f05961 viable and fertile *F FBti0041991 PBac{WH}CG5640f01321 *F FBti0042040 PBac{WH}CG5660f01783 *F FBti0042426 PBac{WH}CG5719f05138 viable and fertile *F FBti0042545 PBac{WH}CG5765f06287 viable and fertile *F FBti0042693 PBac{WH}CG5787f07726 viable and fertile *F FBti0042389 PBac{WH}CG5790f04763 viable and fertile *F FBti0041864 PBac{WH}CG5791f00152 viable and fertile *F FBti0042406 PBac{WH}CG5823f04935 viable and fertile *F FBti0042507 PBac{WH}CG5863f05947 viable and fertile *F FBti0042489 PBac{WH}CG5877f05770 viable and fertile *F FBti0042622 PBac{WH}CG5888f07042 viable and fertile *F FBti0042064 PBac{WH}CG5892f01976 viable and fertile *F FBti0042291 PBac{WH}CG5973f03909 viable and fertile *F FBti0042633 PBac{WH}CG6005f07117 *F FBti0042613 PBac{WH}CG6015f06954 viable and fertile *F FBti0041975 PBac{WH}CG6090f01091 *F FBti0042628 PBac{WH}CG6113f07089 viable and fertile *F FBti0042524 PBac{WH}CG6133f06125 viable and fertile *F FBti0041889 PBac{WH}CG6164f00379 viable and fertile *F FBti0041970 PBac{WH}CG6194f01043 viable and fertile *F FBti0041966 PBac{WH}CG6196f00985 *F FBti0042617 PBac{WH}CG6208f06990 viable and fertile *F FBti0041981 PBac{WH}CG6231f01139 viable and fertile *F FBti0042643 PBac{WH}CG6254f07188 *F FBti0042409 PBac{WH}CG6276f04942 viable and fertile *F FBti0041929 PBac{WH}CG6296f00739 viable and fertile *F FBti0042229 PBac{WH}CG6425f03393 viable and fertile *F FBti0042204 PBac{WH}CG6459f03188 viable and fertile *F FBti0042521 PBac{WH}CG6470f06090 viable and fertile *F FBti0042192 PBac{WH}CG6475f03067 viable and fertile *F FBti0042644 PBac{WH}CG6607f07191 viable and fertile *F FBti0042024 PBac{WH}CG6608f01677 *F FBti0042046 PBac{WH}CG6637f01832 *F FBti0042548 PBac{WH}CG6686f06314 *F FBti0042069 PBac{WH}CG6694f01996 viable and fertile *F FBti0041969 PBac{WH}CG6784f01032 viable and fertile *F FBti0042419 PBac{WH}CG6813f05030 viable and fertile *F FBti0041938 PBac{WH}CG6866f00791 *F FBti0042247 PBac{WH}CG6888f03548 viable and fertile *F FBti0042039 PBac{WH}CG6910f01770 viable and fertile *F FBti0042163 PBac{WH}CG6919f02819 viable and fertile *F FBti0041895 PBac{WH}CG6931f00440 *F FBti0042150 PBac{WH}CG6946f02674 *F FBti0042506 PBac{WH}CG6962f05936 viable and fertile *F FBti0042481 PBac{WH}CG6989f05679 viable and fertile *F FBti0042637 PBac{WH}CG7051f07138 viable and fertile *F FBti0042595 PBac{WH}CG7059f06779 viable and fertile *F FBti0042262 PBac{WH}CG7077f03691 viable and fertile *F FBti0042053 PBac{WH}CG7081f01899 *F FBti0042415 PBac{WH}CG7091f05023 viable and fertile *F FBti0041983 PBac{WH}CG7101f01197 viable and fertile *F FBti0041971 PBac{WH}CG7112f01044 viable and fertile *F FBti0042083 PBac{WH}CG7144f02099 viable and fertile *F FBti0042035 PBac{WH}CG7158f01748 viable and fertile *F FBti0042209 PBac{WH}CG7191f03241 viable and fertile *F FBti0042276 PBac{WH}CG7202f03815 *F FBti0042365 PBac{WH}CG7221f04545 viable and fertile *F FBti0042657 PBac{WH}CG7267f07307 viable and fertile *F FBti0042508 PBac{WH}CG7304f05948 viable and fertile *F FBti0042684 PBac{WH}CG7312f07652 viable and fertile *F FBti0041921 PBac{WH}CG7323f00650 viable and fertile *F FBti0041907 PBac{WH}CG7357f00521 viable and fertile *F FBti0042084 PBac{WH}CG7371f02109 *F FBti0042264 PBac{WH}CG7536f03720 viable and fertile *F FBti0042431 PBac{WH}CG7593f05191 viable and fertile *F FBti0042535 PBac{WH}CG7609f06205 viable and fertile *F FBti0041992 PBac{WH}CG7627f01338 viable and fertile *F FBti0042052 PBac{WH}CG7632f01890 *F FBti0041994 PBac{WH}CG7635f01352 viable and fertile *F FBti0042552 PBac{WH}CG7635f06342 viable and fertile *F FBti0042253 PBac{WH}CG7668f03604 viable and fertile *F FBti0042683 PBac{WH}CG7692f07642 viable and fertile *F FBti0042315 PBac{WH}CG7720f04152 viable and fertile *F FBti0042097 PBac{WH}CG7747f02221 viable and fertile *F FBti0042576 PBac{WH}CG7755f06581 *F FBti0042106 PBac{WH}CG7759f02286 viable and fertile *F FBti0042664 PBac{WH}CG7772f07379 viable and fertile *F FBti0042530 PBac{WH}CG7785f06154 viable and fertile *F FBti0042074 PBac{WH}CG7810f02044 *F FBti0042169 PBac{WH}CG7816f02876 *F FBti0042373 PBac{WH}CG7837f04616 viable and fertile *F FBti0042443 PBac{WH}CG7837f05400 viable and fertile *F FBti0041852 PBac{WH}CG7861f00024 *F FBti0042455 PBac{WH}CG7882f05479 viable and fertile *F FBti0041974 PBac{WH}CG7918f01078 *F FBti0042723 PBac{WH}CG7985f08065 viable and fertile *F FBti0042700 PBac{WH}CG8008f07760 viable and fertile *F FBti0042675 PBac{WH}CG8060f07570 viable and fertile *F FBti0042207 PBac{WH}CG8086f03214 viable and fertile *F FBti0041967 PBac{WH}CG8138f00992 viable and fertile *F FBti0041900 PBac{WH}CG8152f00473 viable and fertile *F FBti0041861 PBac{WH}CG8232f00130 viable and fertile *F FBti0042230 PBac{WH}CG8270f03412 *F FBti0042540 PBac{WH}CG8282f06259 viable and fertile *F FBti0041917 PBac{WH}CG8358f00626 viable and fertile *F FBti0042206 PBac{WH}CG8389f03200 viable and fertile *F FBti0042647 PBac{WH}CG8412f07214 *F FBti0042271 PBac{WH}CG8464f03785 *F FBti0042332 PBac{WH}CG8552f04269 viable and fertile *F FBti0042295 PBac{WH}CG8557f03948 viable and fertile *F FBti0042211 PBac{WH}CG8777f03251 *F FBti0042054 PBac{WH}CG8784f01901 viable and fertile *F FBti0042463 PBac{WH}CG8791f05501 viable and fertile *F FBti0042137 PBac{WH}CG8795f02573 viable and fertile *F FBti0042671 PBac{WH}CG8850f07564 viable and fertile *F FBti0042528 PBac{WH}CG8861f06147 *F FBti0042107 PBac{WH}CG8908f02292 viable and fertile *F FBti0042722 PBac{WH}CG8945f08057 viable and fertile *F FBti0042696 PBac{WH}CG8950f07741 viable and fertile *F FBti0042316 PBac{WH}CG8965f04153 viable and fertile *F FBti0041956 PBac{WH}CG8998f00911 viable and fertile *F FBti0042189 PBac{WH}CG9018f03052 viable and fertile *F FBti0042268 PBac{WH}CG9063f03772 viable and fertile *F FBti0041990 PBac{WH}CG9067f01295 viable and fertile *F FBti0042217 PBac{WH}CG9135f03307 viable and fertile *F FBti0042470 PBac{WH}CG9207f05565 *F FBti0042402 PBac{WH}CG9231f04902 viable and fertile *F FBti0041942 PBac{WH}CG9249f00835 *F FBti0041950 PBac{WH}CG9249f00877 viable and fertile *F FBti0041931 PBac{WH}CG9286f00745 viable and fertile *F FBti0041884 PBac{WH}CG9288f00354 viable and fertile *F FBti0042285 PBac{WH}CG9293f03884 *F FBti0042318 PBac{WH}CG9296f04175 *F FBti0042411 PBac{WH}CG9312f04951 viable and fertile *F FBti0042449 PBac{WH}CG9361f05437 viable and fertile *F FBti0042668 PBac{WH}CG9386f07521 viable and fertile *F FBti0042236 PBac{WH}CG9444f03469 viable and fertile *F FBti0041877 PBac{WH}CG9466f00306 viable and fertile *F FBti0042607 PBac{WH}CG9541f06873 viable and fertile *F FBti0041951 PBac{WH}CG9555f00882 viable and fertile *F FBti0041945 PBac{WH}CG9582f00857 viable and fertile *F FBti0042200 PBac{WH}CG9592f03144 viable and fertile *F FBti0042686 PBac{WH}CG9620f07676 viable and fertile *F FBti0042174 PBac{WH}CG9634f02939 viable and fertile *F FBti0042544 PBac{WH}CG9662f06279 viable and fertile *F FBti0042618 PBac{WH}CG9669f07000 *F FBti0042452 PBac{WH}CG9698f05449 viable and fertile *F FBti0042277 PBac{WH}CG9733f03827 viable and fertile *F FBti0042162 PBac{WH}CG9853f02811 viable and fertile *F FBti0042284 PBac{WH}CG9876f03881 viable and fertile *F FBti0042032 PBac{WH}CG9918f01726 viable and fertile *F FBti0042047 PBac{WH}CG9922f01835 *F FBti0042310 PBac{WH}CG9940f04110 *F FBti0042638 PBac{WH}CG9977f07141 viable and fertile *F FBti0042414 PBac{WH}CG9986f04999 viable and fertile *F FBti0042461 PBac{WH}CG9987f05494 viable and fertile *F FBti0042167 PBac{WH}CSN3f02855 *F FBti0041878 PBac{WH}Cad74Af00312 viable and fertile *F FBti0042459 PBac{WH}Camtaf05489 viable and fertile *F FBti0041949 PBac{WH}Cbp53Ef00876 viable and fertile *F FBti0042466 PBac{WH}Cdk9f05537 *F FBti0042688 PBac{WH}CheA29af07701 viable and fertile *F FBti0042598 PBac{WH}Clkf06808 *F FBti0042709 PBac{WH}Csatf07903 viable and fertile *F FBti0041908 PBac{WH}Cyp12a4f00523 viable and fertile *F FBti0042108 PBac{WH}Cyp301a1f02301 viable and fertile *F FBti0042067 PBac{WH}Cyp318a1f01983 viable and fertile *F FBti0042441 PBac{WH}Cyp6v1f05362 viable and fertile *F FBti0042042 PBac{WH}Cyp9f2f01799 viable and fertile *F FBti0042179 PBac{WH}DNApol-alpha50f02992 *F FBti0042095 PBac{WH}Dammf02209 viable and fertile *F FBti0042574 PBac{WH}Dcr-2f06544 *F FBti0042421 PBac{WH}Ddx1f05056 viable and fertile *F FBti0041926 PBac{WH}Dip-Cf00706 *F FBti0042482 PBac{WH}Dip2f05703 viable and fertile *F FBti0042666 PBac{WH}Dlic2f07409 viable and fertile *F FBti0041885 PBac{WH}DppIIIf00363 viable and fertile *F FBti0042410 PBac{WH}Eig71Ehf04943 viable and fertile *F FBti0042727 PBac{WH}Eip93Ff08111 viable and fertile *F FBti0042669 PBac{WH}Enof07543 *F FBti0042257 PBac{WH}Ets21Cf03639 viable and fertile *F FBti0042381 PBac{WH}Ets98Bf04695 viable and fertile *F FBti0042125 PBac{WH}Fkbp13f02452 viable and fertile *F FBti0042525 PBac{WH}Frqf06131 viable and fertile *F FBti0041953 PBac{WH}Fsf00897 viable and fertile *F FBti0042269 PBac{WH}FucTAf03774 viable and fertile *F FBti0041930 PBac{WH}GXIVsPLA2f00744 viable and fertile *F FBti0042325 PBac{WH}Galpha49Bf04219 *F FBti0042036 PBac{WH}Glu-RIBf01757 *F FBti0042445 PBac{WH}Glu-RIf05411 viable and fertile *F FBti0041982 PBac{WH}Gr2af01153 viable and fertile *F FBti0042026 PBac{WH}Gr93af01688 viable and fertile *F FBti0042536 PBac{WH}Gr97af06215 viable and fertile *F FBti0042458 PBac{WH}Grip75f05483 viable and fertile *F FBti0042124 PBac{WH}Gs1lf02438 *F FBti0041912 PBac{WH}GstD2f00583 viable and fertile *F FBti0042616 PBac{WH}GstD9f06984 *F FBti0042157 PBac{WH}GstE9f02746 viable and fertile *F FBti0042288 PBac{WH}Handf03901 *F FBti0042312 PBac{WH}Hexo2f04129 viable and fertile *F FBti0042584 PBac{WH}Heyf06656 *F FBti0042569 PBac{WH}Hlcf06483 viable and fertile *F FBti0042225 PBac{WH}Hmlf03374 viable and fertile *F FBti0042294 PBac{WH}Hphf03923 viable and fertile *F FBti0042346 PBac{WH}Hrb27Cf04375 *F FBti0042197 PBac{WH}ImpE1f03112 viable and fertile *F FBti0042057 PBac{WH}Jafrac2f01922 viable and fertile *F FBti0042297 PBac{WH}Klp10Af03956 viable and fertile *F FBti0042309 PBac{WH}Lim1f04087 *F FBti0042610 PBac{WH}Lip2f06907 viable and fertile *F FBti0042483 PBac{WH}MICAL-likef05713 viable and fertile *F FBti0042235 PBac{WH}Mcm7f03462 viable and fertile *F FBti0041879 PBac{WH}Met75Caf00316 *F FBti0042436 PBac{WH}Mipp1f05233 viable and fertile *F FBti0042299 PBac{WH}Myo10Af03968 viable and fertile *F FBti0042252 PBac{WH}NLazf03602 *F FBti0042384 PBac{WH}Nakf04720 viable and fertile *F FBti0042128 PBac{WH}Nosf02469 viable and fertile *F FBti0042151 PBac{WH}Obp44af02697 viable and fertile *F FBti0042263 PBac{WH}Or10af03694 viable and fertile *F FBti0042077 PBac{WH}Or35af02057 *F FBti0042339 PBac{WH}Or42af04305 viable and fertile *F FBti0042487 PBac{WH}Or85df05736 viable and fertile *F FBti0041963 PBac{WH}Os-Ef00970 viable and fertile *F FBti0042547 PBac{WH}Os9f06305 viable and fertile *F FBti0041911 PBac{WH}PH4alphaMPf00581 viable and fertile *F FBti0042446 PBac{WH}PQBP-1f05427 viable and fertile *F FBti0042152 PBac{WH}PTP-ERf02707 viable and fertile *F FBti0042164 PBac{WH}Pcaff02830 *F FBti0042453 PBac{WH}Pcaff05456 *F FBti0041965 PBac{WH}Pcmtf00979 viable and fertile *F FBti0042716 PBac{WH}Ppt1f08013 viable and fertile *F FBti0042512 PBac{WH}Prp18f05974 *F FBti0041972 PBac{WH}Ptpmegf01047 *F FBti0042649 PBac{WH}Pxnf07229 viable and fertile *F FBti0042692 PBac{WH}Rab30f07714 *F FBti0042296 PBac{WH}Rab40f03950 viable and fertile *F FBti0042099 PBac{WH}Rad1f02242 viable and fertile *F FBti0042180 PBac{WH}Rdlf02994 *F FBti0042360 PBac{WH}Reg-3f04505 viable and fertile *F FBti0042568 PBac{WH}RhoGAP5Af06460 viable and fertile *F FBti0042681 PBac{WH}RhoGAP88Cf07633 *F FBti0042142 PBac{WH}RpL23Af02629 viable and fertile *F FBti0042226 PBac{WH}RpS9f03376 viable and fertile *F FBti0042438 PBac{WH}S1Pf05271 viable and fertile *F FBti0042712 PBac{WH}SKf07979 viable and fertile *F FBti0042472 PBac{WH}SMC2f05586 viable and fertile *F FBti0042604 PBac{WH}SMC2f06842 *F FBti0042066 PBac{WH}Scgbetaf01979 *F FBti0042422 PBac{WH}Scrf05078 *F FBti0042231 PBac{WH}Ser12f03416 *F FBti0042672 PBac{WH}Shf07565 viable and fertile *F FBti0041904 PBac{WH}Shalf00495 *F FBti0042641 PBac{WH}Sirt7f07159 viable and fertile *F FBti0042620 PBac{WH}Socs44Af07030 viable and fertile *F FBti0042565 PBac{WH}Sox21bf06429 viable and fertile *F FBti0042088 PBac{WH}Spn1f02145 *F FBti0042333 PBac{WH}Sptrf04272 viable and fertile *F FBti0042186 PBac{WH}Ssef03027 viable and fertile *F FBti0042348 PBac{WH}Synf04401 viable and fertile *F FBti0042063 PBac{WH}Syx17f01971 viable and fertile *F FBti0041978 PBac{WH}TMS1f01124 viable and fertile *F FBti0042612 PBac{WH}Taf6f06930 *F FBti0042161 PBac{WH}Takr99Df02797 viable and fertile *F FBti0041867 PBac{WH}Tbpf00190 *F FBti0042041 PBac{WH}Tequilaf01792 viable and fertile *F FBti0042538 PBac{WH}Tkf06233 *F FBti0042028 PBac{WH}TotCf01700 *F FBti0041961 PBac{WH}Trfpf00955 *F FBti0042056 PBac{WH}Tsc1f01910 *F FBti0042424 PBac{WH}Tsf1f05108 viable and fertile *F FBti0042223 PBac{WH}Tsp42Eif03340 viable and fertile *F FBti0041957 PBac{WH}Uchf00912 viable and fertile *F FBti0042494 PBac{WH}Ulp1f05808 viable and fertile *F FBti0042400 PBac{WH}Urof04888 viable and fertile *F FBti0042335 PBac{WH}Vangf04290 *F FBti0042251 PBac{WH}Vha14f03593 viable and fertile *F FBti0042602 PBac{WH}Vm32Ef06827 viable and fertile *F FBti0042261 PBac{WH}XRCC1f03685 viable and fertile *F FBti0042027 PBac{WH}absf01698 *F FBti0042670 PBac{WH}acj6f07555 viable and fertile *F FBti0042385 PBac{WH}adatf04739 viable and fertile *F FBti0042331 PBac{WH}alf04261 viable and fertile *F FBti0042368 PBac{WH}alienf04576 viable and fertile *F FBti0042219 PBac{WH}amdf03321 *F FBti0042556 PBac{WH}amxf06362 viable and fertile *F FBti0041924 PBac{WH}beat-IIaf00670 viable and fertile *F FBti0042605 PBac{WH}beat-Iaf06843 viable and fertile *F FBti0042388 PBac{WH}beat-Ibf04746 viable and fertile *F FBti0042146 PBac{WH}beat-Vbf02649 viable and fertile *F FBti0041888 PBac{WH}beta4GalTBf00376 *F FBti0041866 PBac{WH}bru-2f00171 viable and fertile *F FBti0041984 PBac{WH}bvesf01200 viable and fertile *F FBti0041937 PBac{WH}captf00786 *F FBti0042104 PBac{WH}capuf02268 *F FBti0042440 PBac{WH}cinf05298 *F FBti0042490 PBac{WH}cmf05774 viable and fertile *F FBti0042350 PBac{WH}dahf04432 viable and fertile *F FBti0041998 PBac{WH}dalaof01375 viable and fertile *F FBti0042068 PBac{WH}dallyf01984 viable and fertile *F FBti0042328 PBac{WH}domf04230 viable and fertile *F FBti0041858 PBac{WH}dsff00109 *F FBti0042362 PBac{WH}dyf04509 viable and fertile *F FBti0042505 PBac{WH}eIF-5Cf05932 viable and fertile *F FBti0041939 PBac{WH}elkf00820 *F FBti0041897 PBac{WH}esnf00447 *F FBti0041865 PBac{WH}fasf00157 viable and fertile *F FBti0042016 PBac{WH}fauf01541 viable and fertile *F FBti0042498 PBac{WH}formin3f05866 viable and fertile *F FBti0042345 PBac{WH}gusf04367 viable and fertile *F FBti0041854 PBac{WH}hayf00028 *F FBti0041913 PBac{WH}hbf00586 viable and fertile *F FBti0042214 PBac{WH}herf03287 viable and fertile *F FBti0042587 PBac{WH}higf06695 viable and fertile *F FBti0041896 PBac{WH}hoe1f00446 viable and fertile *F FBti0042090 PBac{WH}ire-1f02170 *F FBti0042011 PBac{WH}kappaB-Rasf01498 viable and fertile *F FBti0042517 PBac{WH}karf06017 viable and fertile *F FBti0042464 PBac{WH}kek5f05515 viable and fertile *F FBti0042055 PBac{WH}knkf01902 *F FBti0042518 PBac{WH}l(1)G0237f06049 viable and fertile *F FBti0042168 PBac{WH}lblf02857 viable and fertile *F FBti0042093 PBac{WH}lectin-22Cf02193 viable and fertile *F FBti0042320 PBac{WH}ligatinf04182 viable and fertile *F FBti0042511 PBac{WH}mRpL1f05962 *F FBti0042044 PBac{WH}mRpL21f01801 *F FBti0042586 PBac{WH}mRpL24f06692 *F FBti0042567 PBac{WH}mRpL3f06448 viable and fertile *F FBti0042479 PBac{WH}mRpL43f05643 *F FBti0042037 PBac{WH}mRpS33f01766 *F FBti0042224 PBac{WH}mRpS35f03356 *F FBti0042112 PBac{WH}mab-2f02334 viable and fertile *F FBti0041996 PBac{WH}mei-9f01366 viable and fertile *F FBti0042661 PBac{WH}mstf07363 viable and fertile *F FBti0042131 PBac{WH}mtSSBf02505 viable and fertile *F FBti0042221 PBac{WH}mus209f03331 viable and fertile *F FBti0042342 PBac{WH}muskelinf04338 viable and fertile *F FBti0041948 PBac{WH}nAcRalpha-34Ef00872 *F FBti0042656 PBac{WH}nesf07294 viable and fertile *F FBti0042330 PBac{WH}netf04249 *F FBti0042304 PBac{WH}nodf04008 viable and fertile *F FBti0041947 PBac{WH}nonAf00870 viable and fertile *F FBti0042509 PBac{WH}nxf2f05960 *F FBti0042703 PBac{WH}ogref07788 viable and fertile *F FBti0042135 PBac{WH}olf413f02553 *F FBti0042543 PBac{WH}orf06278 *F FBti0042462 PBac{WH}pief05500 *F FBti0042314 PBac{WH}pkf04137 viable and fertile *F FBti0042626 PBac{WH}pkf07080 viable and fertile *F FBti0042060 PBac{WH}plef01945 *F FBti0042118 PBac{WH}pngf02367 viable and fertile *F FBti0042334 PBac{WH}pnrf04283 viable and fertile *F FBti0042720 PBac{WH}porf08025 viable and fertile *F FBti0042255 PBac{WH}porinf03616 viable and fertile *F FBti0042075 PBac{WH}ppk11f02053 viable and fertile *F FBti0042427 PBac{WH}prodf05153 viable and fertile *F FBti0042232 PBac{WH}prominin-likef03422 *F FBti0042393 PBac{WH}pwnf04831 viable and fertile *F FBti0042020 PBac{WH}qmf01604 viable and fertile *F FBti0042560 PBac{WH}regucalcinf06398 viable and fertile *F FBti0042450 PBac{WH}rev7f05442 *F FBti0042050 PBac{WH}rnf01856 viable and fertile *F FBti0042290 PBac{WH}roblf03905 viable and fertile *F FBti0042307 PBac{WH}rokf04065 *F FBti0042496 PBac{WH}scuf05851 viable and fertile *F FBti0042193 PBac{WH}sec10f03085 *F FBti0042533 PBac{WH}sensf06181 *F FBti0042116 PBac{WH}sevf02355 viable and fertile *F FBti0041868 PBac{WH}shakBf00199 viable and fertile *F FBti0041925 PBac{WH}sidef00677 *F FBti0042435 PBac{WH}simf05216 viable and fertile *F FBti0042502 PBac{WH}slof05915 viable and fertile *F FBti0042317 PBac{WH}smi35Af04169 viable and fertile *F FBti0042058 PBac{WH}spn-Df01933 viable and fertile *F FBti0042706 PBac{WH}ssf07846 viable and fertile *F FBti0041955 PBac{WH}stanf00907 viable and fertile *F FBti0041997 PBac{WH}swaf01372 viable and fertile *F FBti0041987 PBac{WH}timf01253 viable and fertile *F FBti0042218 PBac{WH}tocf03317 viable and fertile *F FBti0042639 PBac{WH}tstf07143 viable and fertile *F FBti0042625 PBac{WH}unc-13f07072 viable and fertile *F FBti0042575 PBac{WH}ushf06555 *F FBti0042003 PBac{WH}vapf01404 viable and fertile *F FBti0042154 PBac{WH}vgf02736 *F FBti0042079 PBac{WH}wrapperf02069 viable and fertile *F FBti0042311 PBac{WH}xmas-2f04114 *F FBti0042977 P{XP}Adgf-Ad11616 viable and fertile *F FBti0042798 P{XP}AnnXd03410 viable and fertile *F FBti0042856 P{XP}Antpd06610 *F FBti0042839 P{XP}CCR4d06069 viable and fertile *F FBti0042780 P{XP}CG10026d02517 viable and fertile *F FBti0042915 P{XP}CG10262d08604 viable and fertile *F FBti0042867 P{XP}CG10311d06928 viable and fertile *F FBti0042771 P{XP}CG10333d02040 *F FBti0042733 P{XP}CG10465d00266 viable and fertile *F FBti0042892 P{XP}CG10600d07626 viable and fertile *F FBti0042744 P{XP}CG10650d00783 viable and fertile *F FBti0042776 P{XP}CG11200d02302 *F FBti0042861 P{XP}CG11241d06786 *F FBti0042832 P{XP}CG11426d05846 *F FBti0042822 P{XP}CG11490d05023 viable and fertile *F FBti0042836 P{XP}CG11593d06001 *F FBti0042846 P{XP}CG11739d06383 viable and fertile *F FBti0042831 P{XP}CG11896d05816 *F FBti0042853 P{XP}CG11971d06507 viable and fertile *F FBti0042920 P{XP}CG11984d08881 *F FBti0042931 P{XP}CG12075d09295 viable and fertile *F FBti0042896 P{XP}CG12120d07784 viable and fertile *F FBti0042838 P{XP}CG12233d06039 viable and fertile *F FBti0042816 P{XP}CG12708d04821 viable and fertile *F FBti0042855 P{XP}CG12756d06605 *F FBti0042759 P{XP}CG12857d01332 viable and fertile *F FBti0042939 P{XP}CG13003d09754 viable and fertile *F FBti0042956 P{XP}CG13124d10773 viable and fertile *F FBti0042864 P{XP}CG13369d06819 viable and fertile *F FBti0042758 P{XP}CG13373d01279 viable and fertile *F FBti0042735 P{XP}CG13594d00307 viable and fertile *F FBti0042962 P{XP}CG13659d10859 viable and fertile *F FBti0042952 P{XP}CG13875d10431 viable and fertile *F FBti0042849 P{XP}CG14118d06432 viable and fertile *F FBti0042745 P{XP}CG14290d00809 viable and fertile *F FBti0042865 P{XP}CG14427d06860 viable and fertile *F FBti0042958 P{XP}CG14441d10785 viable and fertile *F FBti0042960 P{XP}CG14801d10828 viable and fertile *F FBti0042819 P{XP}CG15093d04924 viable and fertile *F FBti0042857 P{XP}CG15196d06689 viable and fertile *F FBti0042769 P{XP}CG15236d01980 viable and fertile *F FBti0042957 P{XP}CG15260d10778 viable and fertile *F FBti0042886 P{XP}CG15296d07420 viable and fertile *F FBti0042811 P{XP}CG15445d04454 viable and fertile *F FBti0042847 P{XP}CG15745d06417 viable and fertile *F FBti0042841 P{XP}CG15766d06145 viable and fertile *F FBti0042872 P{XP}CG15784d07076 viable and fertile *F FBti0055015 P{XP}CG1677d02937 *F FBti0042814 P{XP}CG1677d04577 viable and fertile *F FBti0042761 P{XP}CG16969d01514 viable and fertile *F FBti0042738 P{XP}CG17083d00403 viable and fertile *F FBti0042809 P{XP}CG17184d04253 viable and fertile *F FBti0042964 P{XP}CG17544d11041 viable and fertile *F FBti0042900 P{XP}CG18228d07873 viable and fertile *F FBti0042898 P{XP}CG1845d07859 viable and fertile *F FBti0042799 P{XP}CG18522d03463 *F FBti0042824 P{XP}CG18547d05047 viable and fertile *F FBti0042961 P{XP}CG1908d10843 viable and fertile *F FBti0042825 P{XP}CG2909d05081 viable and fertile *F FBti0042793 P{XP}CG2974d03231 viable and fertile *F FBti0042747 P{XP}CG30104d00824 viable and fertile *F FBti0042971 P{XP}CG30185d11316 viable and fertile *F FBti0042817 P{XP}CG30345d04911 viable and fertile *F FBti0042748 P{XP}CG30377d00826 viable and fertile *F FBti0042830 P{XP}CG3056d05625 viable and fertile *F FBti0042976 P{XP}CG3097d11614 viable and fertile *F FBti0042779 P{XP}CG31075d02464 viable and fertile *F FBti0042866 P{XP}CG31121d06890 viable and fertile *F FBti0042972 P{XP}CG31555d11329 viable and fertile *F FBti0042737 P{XP}CG31674d00365 viable and fertile *F FBti0042970 P{XP}CG32352d11266 *F FBti0042777 P{XP}CG32394d02315 viable and fertile *F FBti0042925 P{XP}CG32552d09154 viable and fertile *F FBti0042875 P{XP}CG32645d07124 viable and fertile *F FBti0042752 P{XP}CG32705d00976 viable and fertile *F FBti0042927 P{XP}CG32822d09163 viable and fertile *F FBti0042785 P{XP}CG33047d02798 viable and fertile *F FBti0042894 P{XP}CG33147d07752 viable and fertile *F FBti0042911 P{XP}CG33169d08430 viable and fertile *F FBti0042843 P{XP}CG33174d06177 viable and fertile *F FBti0042937 P{XP}CG3376d09724 viable and fertile *F FBti0042730 P{XP}CG3457d00103 viable and fertile *F FBti0042808 P{XP}CG3556d04151 viable and fertile *F FBti0042842 P{XP}CG3700d06151 *F FBti0042909 P{XP}CG3709d08265 *F FBti0042791 P{XP}CG3726d03097 viable and fertile *F FBti0042765 P{XP}CG3842d01821 viable and fertile *F FBti0042788 P{XP}CG3918d02940 viable and fertile *F FBti0042868 P{XP}CG3940d06973 viable and fertile *F FBti0042891 P{XP}CG4136d07615 viable and fertile *F FBti0042906 P{XP}CG4221d08178 viable and fertile *F FBti0042790 P{XP}CG4302d03020 viable and fertile *F FBti0042833 P{XP}CG4330d05929 viable and fertile *F FBti0042879 P{XP}CG4347d07256 *F FBti0042740 P{XP}CG4398d00549 *F FBti0042973 P{XP}CG4476d11470 viable and fertile *F FBti0042781 P{XP}CG4674d02595 viable and fertile *F FBti0042850 P{XP}CG4674d06455 *F FBti0042729 P{XP}CG4678d00092 viable and fertile *F FBti0042877 P{XP}CG4752d07156 viable and fertile *F FBti0042893 P{XP}CG4914d07633 viable and fertile *F FBti0042905 P{XP}CG4966d08138 viable and fertile *F FBti0042936 P{XP}CG5455d09586 viable and fertile *F FBti0042869 P{XP}CG5489d06996 *F FBti0042823 P{XP}CG5608d05045 viable and fertile *F FBti0042812 P{XP}CG5707d04545 viable and fertile *F FBti0042901 P{XP}CG5928d07891 viable and fertile *F FBti0042978 P{XP}CG5963d11639 viable and fertile *F FBti0042922 P{XP}CG6006d09073 viable and fertile *F FBti0042821 P{XP}CG6023d04993 viable and fertile *F FBti0042890 P{XP}CG6126d07549 viable and fertile *F FBti0042942 P{XP}CG6126d09805 *F FBti0042871 P{XP}CG6149d07058 viable and fertile *F FBti0042887 P{XP}CG6171d07444 *F FBti0042851 P{XP}CG6218d06481 viable and fertile *F FBti0042919 P{XP}CG6227d08806 *F FBti0042794 P{XP}CG6357d03281 *F FBti0042804 P{XP}CG6420d03901 viable and fertile *F FBti0042947 P{XP}CG6560d10234 *F FBti0042932 P{XP}CG6567d09306 viable and fertile *F FBti0042944 P{XP}CG6739d09967 *F FBti0042863 P{XP}CG7371d06812 viable and fertile *F FBti0042923 P{XP}CG7408d09134 viable and fertile *F FBti0042829 P{XP}CG7458d05482 viable and fertile *F FBti0042860 P{XP}CG7702d06784 viable and fertile *F FBti0042736 P{XP}CG7778d00356 viable and fertile *F FBti0042895 P{XP}CG7802d07781 viable and fertile *F FBti0042743 P{XP}CG7942d00740 viable and fertile *F FBti0042975 P{XP}CG8233d11598 *F FBti0042959 P{XP}CG8289d10824 viable and fertile *F FBti0042764 P{XP}CG8316d01774 viable and fertile *F FBti0042966 P{XP}CG8465d11117 viable and fertile *F FBti0042955 P{XP}CG8516d10724 *F FBti0042801 P{XP}CG8550d03669 viable and fertile *F FBti0042786 P{XP}CG8600d02813 viable and fertile *F FBti0042941 P{XP}CG9003d09761 *F FBti0042907 P{XP}CG9016d08241 viable and fertile *F FBti0042929 P{XP}CG9095d09266 viable and fertile *F FBti0042792 P{XP}CG9162d03190 viable and fertile *F FBti0042757 P{XP}CG9164d01239 viable and fertile *F FBti0042795 P{XP}CG9264d03356 viable and fertile *F FBti0042742 P{XP}CG9265d00690 viable and fertile *F FBti0042818 P{XP}CG9603d04921 *F FBti0042914 P{XP}CG9615d08549 *F FBti0042950 P{XP}CG9812d10315 viable and fertile *F FBti0042782 P{XP}Ca-alpha1Td02621 viable and fertile *F FBti0042884 P{XP}Cad96Cad07355 *F FBti0042943 P{XP}CanBd09902 viable and fertile *F FBti0042874 P{XP}Cyp18a1d07122 viable and fertile *F FBti0042815 P{XP}Cyp6a13d04675 viable and fertile *F FBti0042949 P{XP}Cyp6t1d10312 viable and fertile *F FBti0042778 P{XP}Dd02427 viable and fertile *F FBti0042763 P{XP}Eip63F-1d01762 viable and fertile *F FBti0042774 P{XP}Fimd02114 viable and fertile *F FBti0042852 P{XP}Flo-2d06490 viable and fertile *F FBti0042979 P{XP}GalNAc-T2d11662 viable and fertile *F FBti0042912 P{XP}Gapdh2d08479 viable and fertile *F FBti0042820 P{XP}Gef64Cd04957 viable and fertile *F FBti0042903 P{XP}Gr23ad07944 viable and fertile *F FBti0042862 P{XP}GstD3d06796 *F FBti0042858 P{XP}HLHmbetad06732 viable and fertile *F FBti0042837 P{XP}Hsp67Bcd06009 viable and fertile *F FBti0042796 P{XP}IP3K2d03393 viable and fertile *F FBti0042749 P{XP}Idgf4d00895 viable and fertile *F FBti0042910 P{XP}Jheh1d08367 viable and fertile *F FBti0042888 P{XP}Lnkd07478 viable and fertile *F FBti0042924 P{XP}MTA1-liked09140 *F FBti0042965 P{XP}Mdhd11068 *F FBti0042750 P{XP}Notumd00939 *F FBti0042917 P{XP}Nrx-1d08766 viable and fertile *F FBti0042928 P{XP}Ork1d09258 viable and fertile *F FBti0042784 P{XP}Pcdd02688 viable and fertile *F FBti0042921 P{XP}Plapd09025 viable and fertile *F FBti0042935 P{XP}Ranbp21d09486 viable and fertile *F FBti0042934 P{XP}SIP2d09417 viable and fertile *F FBti0042870 P{XP}Slobd07006 viable and fertile *F FBti0042908 P{XP}Spn4d08261 viable and fertile *F FBti0042810 P{XP}Surf4d04274 *F FBti0042953 P{XP}Takl2d10454 viable and fertile *F FBti0042806 P{XP}TepIIId03976 viable and fertile *F FBti0042913 P{XP}TfIIA-Ld08487 *F FBti0042756 P{XP}Tsp42Ejd01125 viable and fertile *F FBti0042734 P{XP}Ubxd00281 *F FBti0042754 P{XP}abod01007 *F FBti0042834 P{XP}bid05964 viable and fertile *F FBti0042880 P{XP}bigmaxd07258 viable and fertile *F FBti0042739 P{XP}brmd00415 viable and fertile *F FBti0042954 P{XP}cryd10630 viable and fertile *F FBti0042889 P{XP}epsilonTryd07542 viable and fertile *F FBti0042885 P{XP}esnd07374 viable and fertile *F FBti0042897 P{XP}fbpd07853 viable and fertile *F FBti0042873 P{XP}htld07110 *F FBti0042876 P{XP}ined07155 viable and fertile *F FBti0042813 P{XP}jpd04563 viable and fertile *F FBti0042768 P{XP}kal-1d01966 viable and fertile *F FBti0042963 P{XP}knid10922 viable and fertile *F FBti0042968 P{XP}kstd11183 *F FBti0042930 P{XP}l(1)dd4d09268 viable and fertile *F FBti0042933 P{XP}lmgd09381 viable and fertile *F FBti0042805 P{XP}miltd03910 viable and fertile *F FBti0042787 P{XP}n-sybd02894 *F FBti0042835 P{XP}nocturnind05983 *F FBti0042844 P{XP}notd06314 *F FBti0042945 P{XP}pdm2d09994 viable and fertile *F FBti0042746 P{XP}pyd3d00812 viable and fertile *F FBti0042751 P{XP}qtcd00941 viable and fertile *F FBti0042753 P{XP}sasd01006 viable and fertile *F FBti0042974 P{XP}sec15d11525 viable and fertile *F FBti0042938 P{XP}sod09734 *F FBti0042840 P{XP}sobd06074 *F FBti0042883 P{XP}sut1d07339 viable and fertile *F FBti0042800 P{XP}tupd03613 *F FBti0042969 P{XP}unc-104d11204 *F FBti0042775 P{XP}yind02176 viable and fertile # *U FBrf0179850 *a Celniker *b S. *t 2004.12.2 *T personal communication to FlyBase *u *F Date: Thu, 2 Dec 2004 11:37:55 \-0800 *F Subject: annotation corrections *F From: Susan Celniker *F To: flybase-updates@morgan.harvard.edu *F CG32596 and CG32734 should be removed. The poly A tails of RH48250 *F and RH69554 (respectively) are encoded in the genome. The evidence *F for these genes is based on cDNA clones that are most probably genomic *F contaminants. *F CG4752 has an annotated intron that is not validated by the evidence *F (the orf is encoded through the intron). RE08455 and RH09675 both show *F no intron. *F Sue Celniker # *U FBrf0179851 *a Robertson *b H. *t 2004.10.13 *T personal communication to FlyBase *u FlyBase error report for CG12906 on Wed Oct 13 21:16:49 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Wed, 13 Oct 2004 21:16:49 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG12906 on Wed Oct 13 21:16:49 2004 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG12906 *F Gene annotation error *F Gene CG12906 corresponds to FBgn0041242 *F Comments: Comparison with yakuba reveals that the four groups who originally *F annotated these Grs somehow missed a 40bp upstream possible N-terminal *F extension. My previous work comparing with pseudoobscura did not show this *F because this is a highly trashed pseudogene in Dp. I propose therefore adding *F 40aa to the N-terminus of Gr47a, as below *F MTPFIAPLVMNNQTINTGLIGLTQRPIKQAVKQIQLAVLRMAFTSSQLCSLLTKFTALNGLNTYYFDTKTNAFRVSSKLK *F IYCAIHHALCVLALAHMSYSTASNLRVSVTVLTIGGTMACCVKSCWEKAQGIRNLARGLVTMEQKYFAGRPSGLLLKCRY *F YIKITFGSITLLRIHLIQPIYMRRLLPSQFYLNVGAYWLLYNMLLAAVLGFYFLLWEMCRIQKLINDQMTLILARSGQRN *F RLKKMQHCLRLYSKLLLLCDQFNSQLGHVAIWVLACKSWCQITFGYEIFQMVAAPKSIDLTMSMRVFVIFTYIFDAMNLF *F LGTDISELFSTFRADSQRILRETSRLDRLLSMFALKLALHPKRVVLLNVFTFDRKLTLTLLAKSTLYTICCLQNDYNKLK *F A # *U FBrf0179852 *a Hashimoto *b C. *t 2004.11.1 *T personal communication to FlyBase *u FlyBase error report for CG1865 on Mon Nov 1 16:43:36 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Mon, 1 Nov 2004 16:43:36 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: carl.hashimoto@yale.edu *F Subject: FlyBase error report for CG1865 on Mon Nov 1 16:43:36 2004 *F Error report from Carl Hashimoto (carl.hashimoto@yale.edu) *F Gene or accession: CG1865 *F cDNA or EST error *F Comments: The published sequence for full-length DGC cDNA RE38703 representing *F the gene CG1865 is missing 1 nucleotide, which leads to disruption of the open *F reading frame (e.g., see the NCBI entry for the translated sequence of *F RE38703--it does not begin with a Met and does not match the predicted CG1865 *F protein sequence). RE38703's sequence shows only 1 C rather than 2 C's *F between positions 436 and 439 in the predicted CG1865 mRNA sequence; 2 C's are *F also seen in the partial sequence for the cDNA GH09090 that overlaps with *F RE38703. The sequence with 2 C's is correct as it translates to give the *F complete ORF that matches the predicted CG1865 protein sequence. The question *F is whether the cDNA RE38703 is actually missing the C or its sequence was *F mis-read. # *U FBrf0179853 *a Noselli *b S. *t 2004.10.11 *T personal communication to FlyBase *u FlyBase error report for CG7981 on Mon Oct 11 11:17:34 2004. *F From: FlyBase-error@rail.bio.indiana.edu *F Date: Mon, 11 Oct 2004 11:17:34 \-0500 (EST) *F To: flybase-updates@morgan.harvard.edu *F Cc: noselli@unice.fr *F Subject: FlyBase error report for CG7981 on Mon Oct 11 11:17:34 2004 *F Error report from Stephane NOSELLI (noselli@unice.fr) *F Gene or accession: CG7981 *F Gene annotation error *F Genes CG7981 and CG12497 should be merged. *F Comments: We think CG7981 and CG12497 are parts of the same gene for the *F following reasons: *F 1) starting observation: we have analysed a series of protein-trap lines *F showing basement membrane localization (the normal localization of *F CG7981/Trol/perlecan). Sequence of the insertion site of these lines indicate *F that they are inserted upstream of the current 5' most exon of CG7981 (around *F position 159000 of AE003424). This suggested to us that the annotation for *F CG7981 (a huge gene) could be only partially accurate. *F Looking at the genome annotation, we found that: *F 2) the 5' most EST (HL01107) used for annotation of CG7981 seem to be non *F coding since several STOP codons are found in the 3 frames. *F 3) similarly, there are currently no EST to confirm the structure of gene *F CG12497 (model mRNA has currently no 5' and 3' UTR). *F 4) CG12497 encodes for 2 LDL domains, which are found in great number in *F CG7981 N-terminus. *F For these reasons, we believe that a new model based on the merge of CG12497 *F and CG7981 for the Trol/perlecan gene should be tested. *F Looking forward to hear from you. *F Thanks. *F Stephane # *U FBrf0179854 *a Bloomington Drosophila Stock Center *b ?. *t 2004.8.16 *T personal communication to FlyBase *u *F Date: Mon, 16 Aug 2004 14:38:36 \-0500 (EST) *F From: Kathy Matthews *F Subject: KG07119 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F Subject: KG07119 *F The insertion-bearing second chromosome of the Gene Disruption Project *F line KG07119, originally thought to be homozygous viable, is homozygous *F lethal. Whether lethality of this chromosome is caused by the insertion *F P{SUPor-P}Vha44KG07119, or a lesion elsewhere on the chromosome, is *F not known. # *U FBrf0179855 *a Cook *b K. *c R. *d Eisman *t 2004.8.24 *T personal communication to FlyBase *u *F Date: Tue, 24 Aug 2004 15:32:45 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GawB}17A expression *F Cc: reisman@bio.indiana.edu *F Enhancer trap expression of P{GawB}17A in the cardia *F Kevin Cook and Robert Eisman *F Indiana University *F When P{GawB}17A was combined with various UAS-GFP reporter constructs, *F intense fluorescence was observed in a band of cells around the cardia *F (proventriculus) in addition to the expression previously reported in glial *F cells and larval salivary glands. The cells expressing GAL4 in adult flies *F corresponded to the region of the cardia described as Zone 5 by David King *F in his article 'Cellular organization and peritrophic membrane formation in *F the cardia (proventriculus) of Drosophila melanogaster' (J. Morph. 196: *F 253-282, 1988; FBrf0048518). According to King, Zone 5 is a region of *F columnar epithelial cells responsible for secreting the third layer of the *F peritrophic membrane. Fluorescence was also observed in a band around the *F cardia in third instar larvae, but the position of the band was slightly *F more posterior than in adults. We suspect that the shape of the cardia *F changes during metamorphosis to bring zone 5 more anterior by further *F involution of the midgut at the hilus of the cardia, but we did not examine *F P{GawB}17A expression during pupal stages to confirm this. We also noted *F expression in adult salivary glands. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179856 *a Tucson Drosophila Species Stock Center *b ?. *t 2004.6.8 *T personal communication to FlyBase *u *F Date: Thu, 2 Sep 2004 13:17:55 \-0500 (EST) *F From: Kathy Matthews *F Subject: Changes/additions from the Tucson stock list *F To: flybase-updates@morgan.harvard.edu *F Cc: matthewk@fly.bio.indiana.edu *F Personal communication from: Tucson Drosophila Species Stock Center, *F University of Arizona *F Stock list dated: 8 June 2004 *F Parsing the Tucson list has turned up a few things that need to be changed in *F or added to FlyBase. *F Symbol changes: *F Current Valid Requested New symbol *F \----------------- \--------------------- *F Dhyd\T(1;Y)340/2 Dhyd\T(1;Y)340-2 / is reserved for homologue *F separator *F Dtex\cinnabar Dtex\cn per Tucson stock list *F Dtex\diminished Dtex\dim per Tucson stock list *F Dtex\gapped Dtex\gp per Tucson stock list *F Dald\white Dald\w per Tucson stock list *F New alleles, per Tucson stock list: *F Dmer\wAT discoverer: Alan Templeton; isolated from a natural *F population *F Dmer\pm1 *F Dmer\v1 *F Dmer\vl1 *F Dmau\y1 discoverer: Jerry Coyne # *U FBrf0179857 *a Schupbach *b T. *t 2004.8 *T personal communication to FlyBase *u *F Date: Thu, 09 Sep 2004 12:18:31 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{dec-1+tWa} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Trudi Schupbach, Princeton University (8/04). *F P{dec-1+tWa}2L is a 2L insertion distal to 40A. *F P{dec-1+tWa}2R is a homozygous viable and fertile, 2R insertion distal to *F 42B. *F P{dec-1+tWa}3L is a homozygous viable and fertile, 3L insertion distal to *F 79DF. *F P{dec-1+tWa}3R is a homozygous viable and fertile, 3R insertion distal to *F 82B. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179858 *a Peifer *b M. *t 2004.9.9 *T personal communication to FlyBase *u *F Date: Thu, 09 Sep 2004 13:35:26 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{arm-GFP} construct and insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Mark Peifer, University of North Carolina (8/04). *F P{arm-GFP} is a derivative of the arm minigene described in Orsulic and *F Peifer, 1996 (J. Cell Biol. 134:1283-1300; FBrf0090729). It was cloned in *F the vector pP{W8}. *F P{arm-GFP}57 is a homozygous viable and fertile, second chromosome insertion. *F P{arm-GFP}83 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179859 *a Van Vactor *b D. *t 2004.8 *T personal communication to FlyBase *u *F Date: Thu, 09 Sep 2004 16:22:40 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Van Vactor insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Davie Van Vactor, Harvard Medical School (8/04). *F P{UAS-Abl.K417N}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Lar.cyto}3 is a homozygous viable and fertile, third chromosome *F insertion. *F P{UAS-Abl.F}2 is a second chromosome insertion. *F P{UAS-Abl.F}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179860 *a Thomas *b J. *t 2004.9.14 *T personal communication to FlyBase *u *F Subject: Annotation *F Date: Tue, 14 Sep 2004 11:31:13 \+0100 *F From: 'Josie Thomas' *F To: *F CG6265 has been designated Nep5 or Neprilysin 5 during the course of our *F research *F Dr Josie Thomas *F Molecular and Cellular Biosciences *F Faculty of Biological Sciences *F Miall Building *F University Of Leeds *F LS2 9JT *F UK *F 0113 3432890 # *U FBrf0179861 *a Baker *b J. *c M. *d Kernan *t 2004.9.21 *T personal communication to FlyBase *u *F To: flybase-updates@morgan.harvard.edu *F From: James Baker *F Subject: uncoordinated *F Date: Tue, 21 Sep 2004 10:33:25 \-0400 *F Dear Colleague, *F We would like to submit an update for the gene CG1501, which we have *F identified as uncoordinated (unc) (Baker et al., Development 131, *F 3411-3422). The alleles unc2, unc17 and unc24 have *F rearrangements *F within the transcription unit, and the alleles unc9, unc25, unc26 *F unc27 and unc28 have single-base changes creating stop codons within *F the ORF. *F 5RACE was used to extend the sequence from the cDNA clone LP08350 by *F 538 bases (appended below); the resulting transcript is 164 bp longer *F than CG1501-RA. The start of the peptide sequence in each sequence is *F identical as the 164 bp contain 5' untranslated sequence. Given these *F data the peptide sequences should be identical but an internal deletion *F of the amino acids 681 VKLRHQAEMLRLSKRQNSWR 700 has made its way into *F the annotation for the corresponding peptide in BDGP giving rise to a *F 1366 (as opposed to our predicted 1386 AA) sequence. We suggest that *F this should be corrected as it is not supported by either the BDGP *F sequence or our independent sequence for the LP08350 cDNA. We have *F recently submitted this data to genbank (AY748349). *F Please let us know if we can provide any more information, *F Regards, *F James Baker, Maurice Kernan *F >unc cDNA (full length) *F TCACATTTCGCTGTTGGTGGTGAGTGTCTTGCGCTCGTGCTCGTTTTACCTGGAAAGTTCAGTTACTCAAAC *F GCGGCGCATGTAAGCGGCGGTCAGAGGTTCTATCCGAGCATACGCAGACCAACTCTCGATCCCATCCGAGCC *F TCGAGTTCCTTCAGGACGGCATGAAGGTTCGCCACGTGCCGGCGAGCACGACAACGTTATCGTTCAGGGCGA *F AGCAGCGGGATGTGGGCGGCATCGGCATGCCTTGCGAGCGGGAGCGGAAGCACGAGGCCGCAAAGCGGGAGC *F GGGAGGAGCAGATTAAGCAGGAGCGGGCCACAGCGGCGTCGGCGACACAGCGGGTGCGCCAGCATAATCGAA *F GGATCAGCACCGACGGCGATCCCGCCAGGCGGAGAATGACGCAGTCCAGGGAGCGACTCCAAGCAACCGGAC *F TGGGCAGACCAGGACCGTGCGGACCATCAAATATAACTAGAGTAACGCGGGGATCATCATTGCCCCCGGAGG *F TTGGAGCACACGGATACCGCACAGGAAGGCCTACCAGAAAGCACGTACCTATTCCTCAGGATCAGATTGAGG *F AGCAGGATGACTCCGATGCCAGGGCGGAACGGCTGAGGCAACAGCTCGAGGAACTGGCCGCGATGTCGGAGC *F AGGAGTTCGAGGTGAAGTTCCGCCAGTGGCTGGACCAGGAGGGGATTGCCCGGGAGATGCACTCTCACCTGC *F GGGTGGAGCTCATTCACTGCTTCAATAATACGGCACTCGGCCAGCTCTTGAGCAAGGCTGCTGGTGTGCAGA *F TGGCTCAGTCCCACGCACTGCTCGTCTCGCCACTGGCGATGGTGCTGCACACTCTGGTGGCGGAGTTTCTGC *F ACTCCCAGAACTGCCACTTCACACTGTCGGTCTTCTGCAGCGAGACGCCGCATCGCAGCAACCTGCCGGACT *F TCCGGAGTCGGCCGGAGTTCCGATTCCAGACGGAGGAACTGCAGAAGGTCGTGGCCGCCATACTGGGAGAAA *F AAGAAGCTCTAGCGGATTCGGAATTTGGAAAGCTGGCGGAGGCCCACTACGAGGAGGATCTGGCTGGACAAA *F CGCAGTGCTTGCTGATGGCTCTTATGCGAACGCTGGTGGAGATCAGGAGGTCCGTGCCTAAGCAGGAGGTGG *F AACAACCGGTCCCTGTCTCTCTGCAAGACACCGGCTGTCAAACGGAGCCCTCTGCCTGCGTGGAAGCTCGTC *F CCGAGGTGGACACCACTGGTCTGTATCAGACGGAGGAGCACGAGCTGATTCTGGGCGCAGATGGCCGAAGTG *F TCTTTGTGGGCGGTCGTGTCTCGCAATCTCTGCATTCCGTGGAGCAGCAGCTTAATCAGCTGATTAAGAATC *F TCCGCCATTTGGCCAAGTCGTGTGCTCCGCCCGTTGAGGTGATATCATCGACAAAATTCGAGGATCTCCTAA *F TGAAGGAGCTGCGCGAAAGGGAGCGACTTTTGAAAGCTGGTCAAGCATTTAACCCGGGCGAACCGGTGATCA *F AAATTGCCACCGGGGACAAGGAAGAGGAGCATGAGGTAACCAGTGGCAGGAACCAGAAAGATGTGGTTCACT *F CCAACCTGGGACCCATTCAAGTGCCAGCCCTGGATGTGACCATTCCTCAGCTGCCCCGTCTCCACAGTGAAC *F AACTGGCCAGCCTGGCCGTGATCCGCCAGTCCCTCGAAAAGGTGCAGAAAAAGAGCCGGCAGCCGCAGGGCC *F GGATGTACGTCTCCATGGAGCGAATGGAGACCCTGATGGGGGATGTGTGCGGCTGTGTCCAACTACTTAGCA *F ACGTTCTCAATCTCTCCATGGAGCAGGAGCACTCTGTGGGCATGCACAAGGGCTTCAAATGGGGCTACCGCG *F AGGGTTTTGCCCACGGCCACTTCATGGGCCTGCAGGAGGGTCAGAAACTGGAGCAGTTGGAACAGAAGAAGT *F GGCAAAAGGAAAGGCCACTGCCCGAGAAGCGGGAAAGCTCCGTGCAAACGGAGAGCACGATACCCACGGCGA *F GTATAGCCACCCAAACATCGAGAAAGTCAGCTCACAATGAAAGTGTCATCACCCAAACGGAGCAAAAGCAGC *F TAAAGGACGCTGGGAATCAGGCTAGTTGCGAGCCGCCAACCTTCCAAAAGTCCTACGAACAGTGGATCCACG *F AGATGCTGCACAGCTCCAGTGGCCAAGTGTTCCTCGAACGGGTGGAGCTCTCCTTGAACAAGGCGCTGGAGC *F TGCAAAAGGTGCGGCTAAATGAACTCTTCCAGGTGAAGTTGCGACACCAGGCCGAGATGCTTCGTCTAAGCA *F AGCGGCAGAACTCCTGGCGGACCCTGTGCAAGCGTGTGGAGCGGGACTCGCAATCGTCAGCGGAGGCGCGCG *F ACCTCGTGCAGCAGATCTTCCGGCTGCTGGAGCACTACGAGGCACACCATCAGCAGCTGGCGGAGAAGATCC *F AACAGACGGAGCTTGCAGCGGAGCAGGCGGCTCGCATCATGCCCATGTGGGCGGATGGAGCTGCTGTGGGCT *F CCGCTTCTGTCAACTGGAACACGGCGATGTCTACCACGCTCATTTCCAGTGGGATCTGCACTCCGCCTCCGG *F CTCAGCCTGCCGCTGATGGTGCGCTGAATGTTGGTAAGACCCATGTGGAACAGCACACGTATCCTGACCTGG *F GATATCCCTATCAATTGTTGGCATTAGCTCCGCCAGGTGGAGTTCCAGTTGCAGGCACCTTCGTGCCAGTGA *F CTCACCTTCCTGCCCACTTGCTTGGGGCTTCTGAACACCCTGTTCCTCTGGTCCCAGTTGCAAGGATCTACA *F GTGATCCTATGAAAACGTCAAGACGACTGCTTTCTCCGAGTAGCGTTCCTGCAGACAATGACAGTGCTCATC *F GCGCAGCCGGCGAAACCAATGCTTTCACATTCCAAGGAGGCCCCGTATCTGGGGATCTGCATCCAACTGAAA *F ATAGAAACCAAGCTCCTGCTACTCAAGGACAACAGTTTCCTGTTCCTTTACCTGTTCCTGCTCCTCGGGGCC *F CTTCACCAAGTGTACAGAGTGTTTTGCCCTCCACTGCATCACCGGCACTTCCGCCGCCCGATGAAACGGTAC *F CTCAACCAGGACCTTCCCAATCTTCTATTCCTTTGAAACCGTCAATGCACTCGGTGGCCACCAACACGATAA *F CTCCAGTGCCTCCAAAGGCGACGCCTCTCAAACCGGTAAATAGCAAAGTTTCAGAAGGTCCGAGCTTTGAGG *F AGGCTCTACTAAGTGCCAAAAGCCGAATGCTGCAGTTGGAACAGGAGAGCGACCTATTGGAACAAAGTTTCC *F TCAGCTACCTGGAGAAAACCAAGTCGAAGACATCCCCCCACAAGTCATCGGAGGATGCCGCAAGGGCCCGGA *F GAATCCGCTCCAGCTGCCTGCGGGAACGAGAGCAGGTGCATCGCACTATGGACGGTTTCCGGGATTGGCATC *F GACGCGTTCGCAAGGAGGACGCAGCTAGTGTGGCTCAACTGGAGGAGCTGCGCAACCAGCGACTTATACCCG *F ATCCCATCTTGGACTCAAAGGGGTCGTCACCGCTGTGGCTATCGGAACTGGAGGAGGGTCACGAACAGGACA *F GCTATCCCTTTACGAATGCCATCACCGAAGCTAGGAACAAGGTGCTGGACAAGATAATGCCAGTCAGAAAGG *F AGGAGAAGAAGGAGAAGCAAAACGTTAAGAAGAATTGGCTAGCAGATTTGCCATTTCAGATTCCTGAGGCAT *F CTCCGAAACAGGATCGCCATCTGACCAATGTTAAGCCCGCTGTAACGCGAAGCAGCAGCAATGGTGAGATGA *F ACGTGCTCCTCCAACGGGCCAAAGATGCCCTCGGCCTGAGAACACCAAACCCACTTCTGGATAGCAGCAGCA *F GCAGTTCCAGTATGGAGCTGCCCTCCATGGGTGCCGGCAGATCGCCCAGTACAAAGTTCCAGCAATCCATGG *F CCAGGATGCAGATGCTCTTTGGCGGCGCAGAGGCCGTAAAACCTACAATGCCCGCCAAAACAGGAAGACCTG *F TGAGTGCACCCACCGCAGCATCGGAACCGGGGTTGTGTGCACATTTGGTGCTCCCGAAACGCCCCCACACGG *F CTCCCACTCTTCACACAATCAACGAAAATGAACCAGCTGTTCATCATTTGGATGTCTCCACTTCCAGTTCTT *F CGCAAAGCACTTTGCCCGGGAGATCGTCGGGCGAAGCTTTCGAGGATTTGGTCATGAGCGCCGTGGCGGATG *F GAGCCCGGCGGCGGAATGCGGAATCTAGCACGGAGGTTTCGTACAGCCAGGCGTTCTGGAAACGCATAAACC *F TGTAAAAAAATCTAGAATAGGTGCTTAATATGTTCAAACTGATTAAGTATATATCTATTTAGGTTCCAAAAC *F TCTCTTTATAGAGCAACAAAATAGTAATAATACAATTAATAAAACTCATTGTTTTTTGGCCAAAAAAAAAAA *F AAAAAAA *F >UNC_protein *F MKVRHVPASTTTLSFRAKQRDVGGIGMPCERERKHEAAKREREEQIKQERATAASATQRVRQHNRRISTDGD *F PARRRMTQSRERLQATGLGRPGPCGPSNITRVTRGSSLPPEVGAHGYRTGRPTRKHVPIPQDQIEEQDDSDA *F RAERLRQQLEELAAMSEQEFEVKFRQWLDQEGIAREMHSHLRVELIHCFNNTALGQLLSKAAGVQMAQSHAL *F LVSPLAMVLHTLVAEFLHSQNCHFTLSVFCSETPHRSNLPDFRSRPEFRFQTEELQKVVAAILGEKEALADS *F EFGKLAEAHYEEDLAGQTQCLLMALMRTLVEIRRSVPKQEVEQPVPVSLQDTGCQTEPSACVEARPEVDTTG *F LYQTEEHELILGADGRSVFVGGRVSQSLHSVEQQLNQLIKNLRHLAKSCAPPVEVISSTKFEDLLMKELRER *F ERLLKAGQAFNPGEPVIKIATGDKEEEHEVTSGRNQKDVVHSNLGPIQVPALDVTIPQLPRLHSEQLASLAV *F IRQSLEKVQKKSRQPQGRMYVSMERMETLMGDVCGCVQLLSNVLNLSMEQEHSVGMHKGFKWGYREGFAHGH *F FMGLQEGQKLEQLEQKKWQKERPLPEKRESSVQTESTIPTASIATQTSRKSAHNESVITQTEQKQLKDAGNQ *F ASCEPPTFQKSYEQWIHEMLHSSSGQVFLERVELSLNKALELQKVRLNELFQVKLRHQAEMLRLSKRQNSWR *F TLCKRVERDSQSSAEARDLVQQIFRLLEHYEAHHQQLAEKIQQTELAAEQAARIMPMWADGAAVGSASVNWN *F TAMSTTLISSGICTPPPAQPAADGALNVGKTHVEQHTYPDLGYPYQLLALAPPGGVPVAGTFVPVTHLPAHL *F LGASEHPVPLVPVARIYSDPMKTSRRLLSPSSVPADNDSAHRAAGETNAFTFQGGPVSGDLHPTENRNQAPA *F TQGQQFPVPLPVPAPRGPSPSVQSVLPSTASPALPPPDETVPQPGPSQSSIPLKPSMHSVATNTITPVPPKA *F TPLKPVNSKVSEGPSFEEALLSAKSRMLQLEQESDLLEQSFLSYLEKTKSKTSPHKSSEDAARARRIRSSCL *F REREQVHRTMDGFRDWHRRVRKEDAASVAQLEELRNQRLIPDPILDSKGSSPLWLSELEEGHEQDSYPFTNA *F ITEARNKVLDKIMPVRKEEKKEKQNVKKNWLADLPFQIPEASPKQDRHLTNVKPAVTRSSSNGEMNVLLQRA *F KDALGLRTPNPLLDSSSSSSSMELPSMGAGRSPSTKFQQSMARMQMLFGGAEAVKPTMPAKTGRPVSAPTAA *F SEPGLCAHLVLPKRPHTAPTLHTINENEPAVHHLDVSTSSSSQSTLPGRSSGEAFEDLVMSAVADGARRRNA *F ESSTEVSYSQAFWKRINL # *U FBrf0179862 *a Andrade *b R. *c J. *d Deal *e M. *f Deal *g K. *h Cook *t 2004.9.20 *T personal communication to FlyBase *u Isolation and characterization of Df(3R)BSC56. *F Date: Mon, 20 Sep 2004 16:22:30 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3R)BSC56 *F Cc: Rachel Andrade , medeal@bio.indiana.edu, *F jedeal@bio.indiana.edu *F Isolation and characterization of Df(3R)BSC56 *F Rachel Andrade, Jennifer Deal, Megan Deal and Kevin Cook *F Bloomington Drosophila Stock Center, Indiana University *F Df(3R)BSC56 was isolated as a P transposase-induced male recombination *F event involving P{hsneo}hhneo56 and P{PZ}pnt07825. The deletion was *F isolated as a st1-ca1 recombinant chromosome from the cross st1 *F Sbsbd-1 es ro1 ca1 females x CyO, H{PDelta2-3}HoP2.1/+; st1 *F P{hsneo}hhneo56/P{PZ}pnt07825 ca1 males. Polytene chromosome squashes *F showed a fusion between the 94E1 band and the 94F1,2 doublet band; *F consequently, the breakpoints are 94E1-2;94F1-2. Df(3R)BSC56 failed to *F complement cnc03921, orbdec, pntDelta88 and Df(3R)BSC55. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179863 *a Roote *b J. *t 2004.9.30 *T personal communication to FlyBase *u *F Cc: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: Exel insertions *F Date: Thu, 30 Sep 2004 11:22:55 \+0100 *F To: Kevin Cook *F Hi Kevin, *F 5 morsels of data for your files: *F htld07110 is lethal over Df(3R)ED5794 *F CG11836f02631 is lethal over Df(3R)ED6220 *F mole02670 is lethal over alleles and deletions of l(2)35Bb (but I *F don't think mol = l(2)35Bb. There is more evidence that l(2)35Bb is *F BG:DS00929.16 = CG31834...e.g. the insertion SH2252 is within *F BG:DS00929.16. mol and CG31834 are 810 bp apart.) *F dynactin-subunit-p25c02174 is a lethal allele of l(2)35Bf. *F elBc01007 is phenotypically weak elbow over a deletion of el. *F So these inserts are now 'verified'. *F John *F Cc: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: Exel insertions *F Date: Thu, 30 Sep 2004 11:42:32 \+0100 *F To: Kevin Cook *F ...and finally CG8516d10724 is lethal over Df(3R)ED5438. *F John # *U FBrf0179864 *a Bloomington Drosophila Stock Center *b ?. *t 2004.9.23 *T personal communication to FlyBase *u *F Date: Thu, 23 Sep 2004 11:10:13 \-0500 (EST) *F From: Kathy Matthews *F Subject: symbol for dynactin-subunit-p25 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F A gene symbol is needed for dynactin-subunit-p25 (FBgn0040228). *F I'll use dyn-p25 unless you tell me that is taken or another *F symbol has been assigned since the 6 Sept 04 FlyBase update. *F \-- Kathy # *U FBrf0179865 *a Bloomington Drosophila Stock Center *b ?. *t 2004.9.23 *T personal communication to FlyBase *u *F Date: Thu, 23 Sep 2004 11:41:06 \-0500 (EST) *F From: Kathy Matthews *F Subject: symbol for 26-29kD-proteinase *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F A gene symbol is needed for 26-29kD-proteinase (FBgn0028967). *F I'll use 26-29-p unless you tell me that is taken or another *F symbol has been assigned since the 6 Sept 04 FlyBase update. *F \-- Kathy # *U FBrf0179866 *a Ryder *b E. *t 2004.9.23 *T personal communication to FlyBase *u *F To: Kathy Matthews *F Cc: e.ryder@gen.cam.ac.uk, Kevin Cook , Rachel Drysdale *F (Genetics) *F From: John Roote *F Subject: Re: RS lines for Bloomington *F Date: Thu, 23 Sep 2004 13:21:48 \+0100 *F I have attached lists made by Ed of the GT lines, their coordinates *F and their nearest genes, and another listing the accession nos. Do let *F me know if this is OK. *F The lines are all on a common isogenic X, 2 and 3 background (but note, *F unfortunately NOT the same background as the Drosdel kit). *F They all carry w1118 and are homozygous, with these exceptions: *F GT-000008 lethal balanced over TM6B, Hu 20/20 Hu *F GT-000078 semi-lethal balanced over TM6B, Hu, CyO floating 18/22 Hu, *F 8/22 Cy non-Hu flies have smaller, darker eyes, disrupted wings, *F reduced bristles. *F GT-000294 lethal CyO and TM6B, Hu 28/29 Cy, 23/29 Hu *F GT-000526 semi-lethal balanced over TM6B, Hu 16/31 flies are Hu *F \---------------------------------------------------------------------------- *F Accession: AJ833932 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000008, clone library P{GT1} *F Within gene mapping: GT-000008 maps within CG8927 *F Accession: AJ833933 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000028, clone library P{GT1} *F Accession: AJ833934 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000078, clone library P{GT1} *F Accession: AJ833935 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000184, clone library P{GT1} *F Accession: AJ833936 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000196, clone library P{GT1} *F Within gene mapping: GT-000196 maps within CG12024 *F Accession: AJ833937 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000230, clone library P{GT1} *F Within gene mapping: GT-000230 maps within eIF4AIII (CG7483). *F Accession: AJ833938 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000270, clone library P{GT1} *F Accession: AJ833939 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000294, clone library P{GT1} *F Within gene mapping: GT-000294 maps within cdc2 (CG5363). *F Accession: AJ833940 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000304, clone library P{GT1} *F Accession: AJ833941 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000327, clone library P{GT1} *F Accession: AJ833942 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000344, clone library P{GT1} *F Accession: AJ833943 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000359, clone library P{GT1} *F Within gene mapping: GT-000359 maps within Rgl (CG8865). *F Accession: AJ833944 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000502, clone library P{GT1} *F Within gene mapping: GT-000502 maps within Gef64C (CG32239). *F Accession: AJ833945 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000504, clone library P{GT1} *F Accession: AJ833946 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000508, clone library P{GT1} *F Accession: AJ833947 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000526, clone library P{GT1} *F Accession: AJ833948 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000530, clone library P{GT1} *F Accession: AJ833949 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000558, clone library P{GT1} *F Within gene mapping: GT-000558 maps within CG3428 *F Accession: AJ833950 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000656, clone library P{GT1} *F Within gene mapping: GT-000656 maps within Sulf1 (CG6725). *F Accession: AJ833951 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000718, clone library P{GT1} *F Within gene mapping: GT-000718 maps within CG11170 *F Accession: AJ833952 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000741, clone library P{GT1} *F Within gene mapping: GT-000741 maps within CG11790 *F Accession: AJ833953 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-000966, clone library P{GT1} *F Within gene mapping: GT-000966 maps within CG8398 *F Accession: AJ833954 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-001020, clone library P{GT1} *F Within gene mapping: GT-001020 maps within CG11523 *F Accession: AJ833955 *F Description: Drosophila melanogaster flanking sequence of P element insertion *F P{GT1}GT-001092, clone library P{GT1} *F \---------------------------------------------------------------------------- *F 'GT-000008','3R','12010099','89B12','backward' *F 'GT-000028','2R','10279890','51E7','backward' *F 'GT-000078','3L','1569497','62A7','backward' *F 'GT-000184','3L','15253128','71C2','backward' *F 'GT-000196','3L','1837849','62B7','backward' *F 'GT-000230','3R','4139040','84F11','forward' *F 'GT-000270','3L','9317612','67A9','backward' *F 'GT-000294','2L','10378501','31D11','backward' *F 'GT-000304','2R','16675716','57F5','forward' *F 'GT-000327','3R','2358893','83F1','backward' *F 'GT-000344','2R','18280568','59D3','forward' *F 'GT-000359','3L','13865525','70C5','backward' *F 'GT-000502','3L','4715059','64B17','backward' *F 'GT-000504','2R','18314029','59D4','forward' *F 'GT-000508','3R','5913216','85F10','forward' *F 'GT-000526','3R','14989783','91F8','forward' *F 'GT-000530','2L','7429656','27F6','forward' *F 'GT-000558','3L','9445276','67B9','forward' *F 'GT-000656','3R','12139961','89B18','backward' *F 'GT-000718','2R','17226371','58C5','forward' *F 'GT-000741','3R','20864105','96B18','forward' *F 'GT-000966','3L','6573043','65C1','forward' *F 'GT-001020','3L','22183932','79D3','backward' *F 'GT-001092','3L','8431575','66D1','backward' *F '\N','GT-000008','CG8925','proximal','4103' *F '\N','GT-000008','CG8927','in_gene','0' *F '\N','GT-000008','CG6046','distal','1799' *F '\N','GT-000008','CG6072','distal','2575' *F '\N','GT-000028','CG8092','proximal','2533' *F '\N','GT-000028','CG8093','proximal','565' *F '\N','GT-000028','CG11808','distal','44' *F '\N','GT-000028','CG12954','distal','857' *F '\N','GT-000028','CG8079','distal','2216' *F '\N','GT-000028','CG8094','distal','2670' *F '\N','GT-000078','CG13917','distal','4394' *F '\N','GT-000184','CG7259','proximal','3357' *F '\N','GT-000184','CG12327','proximal','1509' *F '\N','GT-000184','CG7255','distal','120' *F '\N','GT-000196','CG13936','proximal','4980' *F '\N','GT-000196','CG12024','in_gene','0' *F '\N','GT-000196','CG1956','distal','1894' *F '\N','GT-000230','CG9615','proximal','4681' *F '\N','GT-000230','CG11693','proximal','3369' *F '\N','GT-000230','CG11694','proximal','1218' *F '\N','GT-000230','CG11698','proximal','206' *F '\N','GT-000230','CG7483','in_gene','0' *F '\N','GT-000230','CG9613','distal','1481' *F '\N','GT-000230','CG7459','distal','4315' *F '\N','GT-000270','CG32040','proximal','3564' *F '\N','GT-000270','CG4347','proximal','51' *F '\N','GT-000270','CG32039','distal','121' *F '\N','GT-000270','CG4446','distal','1410' *F '\N','GT-000270','CG10923','distal','3729' *F '\N','GT-000294','CG5091','proximal','3857' *F '\N','GT-000294','CG5096','proximal','1362' *F '\N','GT-000294','CG5363','in_gene','0' *F '\N','GT-000294','CG5108','distal','572' *F '\N','GT-000294','CG5102','distal','1986' *F '\N','GT-000304','CG10080','proximal','904' *F '\N','GT-000304','CG15674','distal','121' *F '\N','GT-000304','CG10321','distal','2503' *F '\N','GT-000327','CG15185','proximal','4613' *F '\N','GT-000327','CG15183','proximal','109' *F '\N','GT-000344','CG3092','proximal','3072' *F '\N','GT-000359','CG8865','in_gene','0' *F '\N','GT-000502','CG32239','in_gene','0' *F '\N','GT-000504','CG12782','proximal','4927' *F '\N','GT-000504','CG13540','distal','123' *F '\N','GT-000504','CG3134','distal','794' *F '\N','GT-000526','CG5637','proximal','3842' *F '\N','GT-000526','CG11779','proximal','4166' *F '\N','GT-000526','CG5558','proximal','1766' *F '\N','GT-000526','CG5557','distal','177' *F '\N','GT-000530','CG5958','proximal','3945' *F '\N','GT-000530','CG5973','distal','81' *F '\N','GT-000558','CG3654','proximal','1215' *F '\N','GT-000558','CG3428','in_gene','0' *F '\N','GT-000558','CG32036','distal','391' *F '\N','GT-000558','CG32037','distal','2051' *F '\N','GT-000656','CG6725','in_gene','0' *F '\N','GT-000718','CG30279','proximal','3292' *F '\N','GT-000718','CG11275','proximal','170' *F '\N','GT-000718','CG11170','in_gene','0' *F '\N','GT-000718','CG5465','distal','326' *F '\N','GT-000718','CG5625','distal','3158' *F '\N','GT-000741','CG31107','proximal','4952' *F '\N','GT-000741','CG31110','proximal','2591' *F '\N','GT-000741','CG11786','proximal','192' *F '\N','GT-000741','CG11790','in_gene','0' *F '\N','GT-000741','CG11791','distal','2066' *F '\N','GT-000966','CG8368','proximal','4754' *F '\N','GT-000966','CG8398','in_gene','0' *F '\N','GT-001020','CG9063','proximal','3759' *F '\N','GT-001020','CG9054','proximal','636' *F '\N','GT-001020','CG11523','in_gene','0' *F '\N','GT-001020','CG6395','distal','607' *F '\N','GT-001092','CG6964','proximal','196' *F '\N','GT-001092','CG6915','distal','142' *F \---------------------------------------------------------------------------- # *U FBrf0179867 *a Bloomington Drosophila Stock Center *b ?. *t 2004.9.24 *T personal communication to FlyBase *u *F Date: Fri, 24 Sep 2004 11:09:26 \-0500 (EST) *F From: Kathy Matthews *F Subject: Formal aberration symbols in FlyBase *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Bloomington Drosophila Stock Center *F The valid symbols for the following aberrations in FlyBase, although formally *F correct, are less meaningful to most of us than the symbols used in the *F Bloomington stock list. It would be helpful if FlyBase would adopt as valid *F the symbol in use at Bloomington for these aberrations. *F Stock Center Symbol FlyBase Symbol FBab *F Df(1)G4eLH24iR In(1)G4eLH24iR FBab0003932 *F Df(1)NP5 In(1)366.2LP363R FBab0026716 *F Df(1)dm75e19 In(1)dm75e19 FBab0000802 *F Df(2L)J69LH56R Ts(YLt;2Lt)J69+Ts(YSt;2Rt)H56 FBab0024808 *F Df(2R)G10-7-5 In(2LR)ltG10LSDS7-5R FBab0022765 *F Df(2R)P14TE In(2LR)P14LTE45FR FBab0010228 *F Df(2R)bwVDe2LPxKR In(2R)bwVDe2LPxKR FBab0005117 *F Df(2R)pk78s In(2R)pk78s FBab0002198 *F Df(3R)A113 Ts(YSt;3Lt)A113 FBab0002482 *F Df(3R)B81 Ts(YLt;3Lt)B81 FBab0010188 *F Df(3R)CbxTwtLUbxKM5RIn(3R)CbxTwtLUbxKM5R FBab0023890 *F Df(3R)R133 Ts(YLt;3Lt)R133 FBab0009005 *F Df(3R)pb36LAntp5R In(3R)pb36LAntp5R FBab0022429 *F Dp(1;3)scJ4 Ts(1Lt;3Rt)scJ4 FBab0006466 *F Dp(3;3)S1 In(3LR)UbxULHR33R FBab0003791 # *U FBrf0179868 *a Parks *b A. *t 2004.9.23 *T personal communication to FlyBase *u More Exelixis deficiencies. *F Date: Thu, 23 Sep 2004 11:33:39 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: More Exelixis deficiencies *F Cc: matthewk@indiana.edu *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Exelixis, Inc. The information was provided by Annette Parks. *F Information about fourteen deletion chromosomes that did not appear in *F Parks et al., 2004 (Nature Genetics 36:288-292; FBrf0175003) is included in *F the attached Excel spreadsheet. *F In addition, the information on one of the Dfs published in Parks et al. *F was not correct. Df(2R)Exel8056 was constructed using P{XP}d09955 (2R, *F 7807732) and PBac{WH}f02736 (2R, 7958786). It was published as *F Df(2L)Exel8056 (FBab0037971) and the cytology given in the paper *F (28C4;28D3) is the cytology that would have been predicted from the *F coordinates had they been 2L rather than 2R coordinates. The predicted *F cytology using the 2R coordinates from Exelixis is 49D1;49E1 (predicted *F from http://flybase.bio.indiana.edu/maps/lk/genome-cyto-seq-map/ for *F Release 3). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu *F __________________________________________________________ *F CSV version of attached spreadsheet: *F 'Df','Chr','Start const','Start insertion','Start orientation','End *F const','End insertion','End orientation','Start Coord','End *F Coord','Size','Resulting ''hybrid'' insertion','Df cytology','Comments' *F 'New Dfs',,,,,,,,,,,,, *F 'Df(2L)Exel6022','2L','XP','d03590','minus','XP','d04563','minus',9439878,95527 *F 12,112834,'P{w+mC=XP-U}Exel6022','30B5;30B11', *F 'Df(3R)Exel6282','3R','XP','d01551 *F   ','minus','XP','d06384','minus',14409990,14566054,156064,'P{w+mC=XP-U}Exel6 *F 282','91B5;91C5', *F 'Df(2R)Exel6285','2R','XP','d04914','plus','XP','d01556','plus',10547139,107399 *F 31,192792,'P{w+mC=XP-U}Exel6285','52A4;52B5', *F 'Df(3R)Exel8159','3R','XP','d06006','plus','WH','f05002','minus',9809255,100851 *F 29,275874,'P+PBac{XP5.WH5}Exel8159','88A4;88B1', *F 'Df(1)Exel9052','X','WH','f06362','plus','XP','d03720','minus',8986055,8991121, *F 5066,'P+PBac{XP5.WH5}Exel9052','8D10;8D10', *F 'Df(1)Exel9053','X','XP','d00034','plus','rB','e02394','plus',11429473,11441116 *F ,11643,'P+PBac{XP5.RB3}Exel9053','10D5;10D6', *F 'Df(1)Exel9054','X','XP','d07122','plus','WH','f06409','minus',18423839,1847219 *F 3,48354,'P+PBac{XP5.WH5}Exel9054','17D1;17D3', *F 'Df(3L)Exel9058','3L','XP','d01686','plus','WH','f03366','minus',4520054,453235 *F 7,12303,'P+PBac{XP5.WH5}Exel9058','64B11;64B11', *F 'Df(2L)Exel9063','2L','WH','f04763','minus','WH','f06810','minus',18272825,1827 *F 7277,4452,'PBac{w+mC=WHr}Exel9063','36E1;36E1', *F 'Df(2L)Exel9064','2L','rB','e04255','minus','rB','e02253','minus',9407703,94236 *F 55,15952,'PBac{w+mC=RBr}Exel9064','30B4;30B5', *F 'Df(3L)Exel9065','3L','rB','e01179','plus','rB','e03403','plus',21434299,214402 *F 93,5994,'PBac{w+mC=RBr}Exel9065','78D5;78D5', *F 'Df(3L)Exel9066','3L','rB','e04049','minus','rB','e03207','minus',21457326,2147 *F 2256,14930,'PBac{w+mC=RBr}Exel9066','78D5;78D6', *F 'Df(1)Exel9067','X','WH','f00224','minus','XP','d02278','plus',18520231,1854326 *F 5,23034,'P+PBac{w+mC=XP3.WH3}Exel9067','17D6;17E1', *F 'Df(1)Exel9068','X','XP','d03564','minus','XP','d05926','minus',18972891,189909 *F 22,18031,'P{w+mC=XP-U}Exel9068','18B4;18B6', *F ,,,,,,,,,,,,, *F 'Correction',,,,,,,,,,,,, *F 'Df(2R)Exel8056','2R','XP','d09955','plus','WH','f02736','minus',7807732,795878 *F 6,151054,'P+PBac{XP5.WH5}Exel8056','49D1;49E1','In paper with 2L instead of *F 2R. d09955 and f02736 mapped to 2R by both Exel and GDP. ' # *U FBrf0179869 *a Beitel *b G. *t 2004.9.21 *T personal communication to FlyBase *u *F Date: Tue, 21 Sep 2004 13:13:02 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Info from Greg Beitel *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Greg Beitel, Northwestern University (8/04). *F I. Information for Df(3L)sinunwu7 (FBab0038192) *F The sequence across the Df(3L)sinunwu7 breakpoint is *F acggttgggcctttt/aagtcagggtcggtc. The deletion can be followed using the *F primers nwu7 left (5'-CCATTCAGACAGCCAGTGACTT-3') and nwu7 right *F (5'-AAAAACGTGCACTACCAGAAACTG-3'). The product should be 759 bp for the *F nwu7 deletion chromosome and 1.4 kb for WT. *F II. TM6B, P{ry+t7.2=HZ2.7}3, Sb1 Tb1 ca1 *F Reference: Paul SM, Ternet M, Salvaterra PM, Beitel GJ.. The Na+/K+ *F ATPase is required for septate junction function and epithelial tube-size *F control in the Drosophila tracheal system. Development. 2003 *F Oct;130(20):4963-74. *F Constructed by: Greg Beitel and Alex Hirschi *F This balancer variant was created by hopping P{HZ2.7} (FBtp0000299) into *F TM6B, AntpHu Sb1 e1 Tb1 ca1 (=TM6B-Sb1; FBba0000282) from *F Bloomington stock 3619 (brm2 es ca1/TM6B, Sb1 Tb1 *F ca1). P{HZ2.7} was obtained from Bill McGinnis. The resulting insertion *F is P{HZ2.7}3. The balancer short name used at Bloomington will be TM6B, *F P{ry+t7.2=HZ2.7}3, Sb1 Tb1 ca1. *F III. CyO, P{w+mC=Dfd-EYFP}2 *F Constructed by: Greg Beitel, ZhiGuo Liang, Stephanie Ray, Marcus Yu and *F Heeren Patel *F Here is the description provided by Greg Beitel: *F 'This is a 'direct drive' (i.e. not Gal4/UAS) fluorescent balancer with *F strong eYFP expression in the head region visible from stage late 14 and *F continuing through adulthood. In late embryos and larva there is expression *F in the posterior spiracles and to some extent the denticle belts. The YFP *F is easily scorable by compound or dissecting microscope. In adults the *F proboscis is scorable. The eYFP can be stained immunohistochemically using *F a standard 25 min 4% formaldehyde fix and a the 3E6 mouse anti-GFP *F monoclonal (Molecular Probes). The w+ has a variable expressivity, but is *F strong enough to be used in screens looking for w- flies. *F The dfd-HIYFP transposon was made using the autoregulatory element of the *F deformed promotor and contains the 2.7 kb genomic Xba fragment named 'Hz2.7 *F REV' that had been cloned by Bergson and McGinnis (EMBO vol. 9 1990) into *F pBluescript. The Hz2.7REV fragment was excised using NotI/BamHI and *F dropped into the NotI/BamHI sites of the pYellow HI-Pelican vector (Beitel *F unpublished ; Genbank accession number AY730637) which is derived from the *F pGreen H Pelican vector of Barolo et al., Biotechniques vol. 29, *F 2000). The pYellow HI-Pelican contains eYFP (Clontech) rather than the *F original eGFP, and a small intron was added between the transcription *F start site and the eYFP coding sequence. The intron was added to test if *F splicing would improve expression, but this intron does not appear to *F significantly improve expression compared to an intronless *F construct (G. Beitel unpublished). The original isolation name of this *F line was dfd::HIYFP4 for those labs that received it prior to it being *F contributed to the stock centers.' *F P{Dfd-EYFP}2 is the insertion in CyO. The balancer short name used at *F Bloomington will be CyO, P{w+mC=Dfd-EYFP}2. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179870 *a Ordway *b R. *t 2004.9 *T personal communication to FlyBase *u P{UAS-cac1-EGFP} insertions. *F Date: Thu, 09 Sep 2004 13:50:36 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-cac1-EGFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Rick Ordway, Pennsylvania State University (9/04). *F P{UAS-cac1-EGFP}786C is homozygous viable and fertile. *F P{UAS-cac1-EGFP}422A is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-cac1-EGFP}471 is an X chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179871 *a Featherstone *b D. *t 2004.10.16 *T personal communication to FlyBase *u *F Date: Sat, 16 Oct 2004 10:42:19 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: annotation update *F comments: brec corresponds to CG18039, a new NMJ glutamate receptor *F subunit which we are calling 'GluRIID'. So you can stick 'brec' under the *F synonyms for that gene and get rid of the seperate entry for 'brec'. The *F manuscript has been submitted; I just wanted to update this entry, since it *F has lingered so long... *F Dave *F \------------------- *F From rd120@gen.cam.ac.uk Tue Mar 05 15:50:41 2002 *F To: featherstone@biology.utah.edu *F Subject: Helping FlyBase: CSH-26 *F Dear David, *F We are currently curating the abstracts for the 2001 CSH Neurobiology of *F Drosophila Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'bad reception (brec) is essential for glutamate receptor field *F formation.' *F You mention a gene that is new to FlyBase, brec. Now that some time *F has passed I wonder do you know which of the Genome Project CG *F annotations your gene corresponds to? All the CGs have corresponding *F gene records in FlyBase already and we don't like to make duplicate *F records for what is actually the same gene unless we can't avoid it. *F Even if you don't know which CG brec is then perhaps you could tell me *F its map location, as this is valuable information in its own right. *F Thank you very much for your help, *F with best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From featherstone@biology.utah.edu Tue Mar 05 17:01:39 2002 *F To: Rachel Drysdale (Genetics) *F Subject: Re: Helping FlyBase: CSH-26 *F Hi Rachel, *F I do not yet know what CG gene 'bad reception' corresponds to, *F although hopefully this will be changing within the next few weeks, *F since we now appear to have some P-inserts. I'll certainly let you *F know the CG gene ASAP once we know. As for map position, the best I *F can tell you is 92E, which as close as we've been able to map it. *F Thanks for the continuing great work on Flybase. *F Dave *F Dave Featherstone *F University of Utah *F Dept. of Biology *F 257 South 1400 East *F Salt Lake City, UT 84112 *F realname: Dave Featherstone *F reply-to: def@uic.edu *F source: FB-NG Help Mail: *F usersubject: annotation update *F Sent from computer 24.13.79.19 # *U FBrf0179872 *a Van Vactor *b D. *t 2004.8 *T personal communication to FlyBase *u UAS-Lar constructs and insertions. *F Date: Thu, 09 Sep 2004 16:36:31 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-Dl::GFP} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Daniel Alexandre, Laboratoire de Dynamique Moleculaire des *F Interactions Membranaires, Universite Montpellier II (10/04). *F P{UAS-Dl::GFP}DA53 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Dl::GFP}DA55 is a homozygous viable and fertile, third chromosome *F insertion. *F P{UAS-Dl::GFP}DA55 is expressed more strongly than P{UAS-Dl::GFP}DA53. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179874 *a Ratnakumar *b K. *c C. *d Desplan *t 2004.10 *T personal communication to FlyBase *u P{longGMR-GAL4}2. *F Date: Thu, 04 Nov 2004 10:26:13 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{longGMR-GAL4}2 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York University *F (10/04). *F P{longGMR-GAL4}2 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179875 *a Alexandre *b D. *t 2004.10 *T personal communication to FlyBase *u P{UAS-ezrin.1-280.GFP}13C5. *F Date: Thu, 04 Nov 2004 11:02:09 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-ezrin.1-280.GFP}13C5 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Daniel Alexandre, Laboratoire de Dynamique Moleculaire des *F Interactions Membranaires, Universite Montpellier II (10/04). *F P{UAS-ezrin.1-280.GFP}13C5 is a homozygous viable and fertile, second *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179876 *a Ratnakumar *b K. *c C. *d Desplan *t 2004.10 *T personal communication to FlyBase *u P{ninaE-EGFP.P} and P{Pan-R7-GAL4} insertions. *F Date: Thu, 04 Nov 2004 11:10:35 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{ninaE-EGFP.P} and P{Pan-R7-GAL4} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York University *F (10/04). *F P{ninaE-EGFP.P}3 is a homozygous viable and fertile, third chromosome *F insertion. *F P{ninaE-EGFP.P}1 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{Pan-R7-GAL4}2 is a second chromosome insertion. *F P{Pan-R7-GAL4}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179877 *a Shravage *b B. *t 2004.11.5 *T personal communication to FlyBase *u *F Date: Fri, 5 Nov 2004 15:55:28 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-I16 *F To: bhupendra.shravage@uni-koeln.de *F Cc: rd120@gen.cam.ac.uk *F Dear Bhupendra, *F We are (belatedly) curating the abstracts for the 18th *F (Gottingen) European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'The role of EGF and TGF-ß signaling in patterning of follicle cells during *F Drosophila oogenesis'. *F You mention a gene symbol that from its symbol looks new to FlyBase, *F snoN. Do you know which of the Genome Project CG annotations your gene *F corresponds to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Fri, 5 Nov 2004 17:30:42 \+0100 *F From: bhupendra shravage *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: EDRC-I16 *F Dear Rachel, *F Thank you for the mail. The CG number for the gene which I spoke about in the *F fly meeting 2003 is 'CG7233' . (We called it 'snoN' because it shows a strong *F homology to human and chicken snoN). I hope this will suffice. *F best *F bhupendra *F bhupendra V. shravage *F Prof. Siegfried Roth AG *F Insitute for Entwicklungsbiologie *F University of Cologne *F D-50923, Cologne *F Germany *F tel: 0049 221 4702487 *F fax: 0049 221 4705164 # *U FBrf0179878 *a Eggert *b H. *t 2004.11.5 *T personal communication to FlyBase *u Helping FlyBase: EDRC-C21. *F Date: Fri, 5 Nov 2004 15:52:44 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-C21 *F To: Harald.Eggert@rz.hu-berlin.de *F Cc: rd120@gen.cam.ac.uk *F Dear Harald, *F We are (belatedly) curating the abstracts for the 18th (Gottingen) *F European Drosophila Research Conference, for FlyBase. I am writing in *F connection with your abstract: *F 'Z4 and Chriz: two novel proteins essentially involved in the structuring of the *F interphase chromosomes'. *F You mention a gene that is new to FlyBase, Z4. Do you know which of *F the Genome Project CG annotations Z4 corresponds to? Is Z4 the same as *F br? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: Harald Eggert *F Date: Fri, 5 Nov 2004 18:40:14 \+0100 (MET) *F Subject: Re: Helping FlyBase: EDRC-C21 *F To: *F Dear Rachel, *F the gene we named Z4 is identical to gene CG7752 annotated by the *F genome project. This and the isolation of Chriz = CG10712 has *F recently been published in J Cell Sci 117, 4253. Concerning Chriz *F there is a confusion as this gene is also called Chromator, though *F a reference describing its isolation has been missing (an abstract *F as a reference is not acceptable). This should also be updated in *F Flybase. *F Best regards *F Harald *F \----------------------------------------------------------------- *F Dr. Harald Eggert *F Humboldt Universitaet *F Institut fuer Biologie *F Abteilung Cytogenetik *F Chausseestrasse 117 *F 10115 Berlin *F Germany # *U FBrf0179879 *a Demontis *b F. *t 2004.11.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-H12. *F Date: Fri, 5 Nov 2004 15:53:27 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-H12 *F To: demontis@mpi-cbg.de *F Cc: rd120@gen.cam.ac.uk *F Dear Fabio, *F We are (belatedly) curating the abstracts for the 18th *F (Gottingen) European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Characterization of a novel Hedgehog target gene in Drosophila'. *F You mention a gene symbol that is new to FlyBase, CA-10. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Sun, 7 Nov 2004 15:37:29 \+0100 (MET) *F Subject: Re: Helping FlyBase: EDRC-H12 *F From: demontis@mpi-cbg.de *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F I am sorry for the confusion. You could substitute the name CA-10 (that is *F the name we assigned during the screening) with the corresponding gene, *F cad99C (CG31009). *F Best regards *F Fabio Demontis # *U FBrf0179880 *a Tselykh *b T. *t 2004.11.7 *T personal communication to FlyBase *u Helping FlyBase: EDRC-H31. *F Date: Fri, 5 Nov 2004 15:54:41 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-H31 *F To: timofei.tselykh@helsinki.fi *F Cc: rd120@gen.cam.ac.uk *F Dear Timofey, *F We are (belatedly) curating the abstracts for the 18th *F (Gottingen) European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Tom Thumb, a novel essential Drosophila protein which plays a role in *F nucleolus and mitochondria'. *F You mention a gene symbol that is new to FlyBase, tmb. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Sun, 7 Nov 2004 14:02:06 \+0200 *F From: Timofey Tselykh *F To: 'Rachel Drysdale (Genetics)' *F Cc: tapio.heino@helsinki.fi *F Subject: Re: Helping FlyBase: EDRC-H31 *F Dear Rachel, *F You can write in your records: *F tom thumb (tmb) corresponds to CG14283. We named the CG14283 tom thumb *F according to larval mutant phenotype and the protein length. *F A publication concerning tmb (CG14283) is currently in preparation. *F Thank you for keeping FlyBase records up to date! *F With best wishes, *F Timofey. *F \-- *F Timofey Tselykh, M.Sci. *F tel.: \+358-9-191-59896 *F Drosophila Research Group *F Developmental Biology Program *F Institute of Biotechnology *F University of Helsinki *F P.O.Box 56 (Viikinkaari 9) *F 00014 Helsinki *F FINLAND # *U FBrf0179881 *a Wilson *b I. *t 2004.11.8 *T personal communication to FlyBase *u Helping FlyBase: EDRC-H34. *F Date: Fri, 5 Nov 2004 15:55:06 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-H34 *F To: iain.wilson@boku.ac.at *F Cc: rd120@gen.cam.ac.uk *F Dear Iain, *F We are (belatedly) curating the abstracts for the upcoming 18th *F (Gottingen) European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Fucosylation and the anti-horseradish peroxidase epitope in Drosophila *F melanogaster'. *F You mention a gene symbol that is new to FlyBase, Fuc-TVIII. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: 'Iain Wilson' *F To: 'Rachel Drysdale (Genetics)' *F Date: Mon, 08 Nov 2004 10:04:37 \+0100 *F Subject: Re: Helping FlyBase: EDRC-H34 *F Dear Rachel, *F Fuc-TVIII is the name based on the mammalian nomenclature for the *F orthologous gene \- about the same time a French group submitted to *F Genbank a sequence of the same cDNA and named it 'FucT6' \- which is *F the name now found in Flybase. There is obviously no need then to add *F another gene symbol. *F With best regards, *F Iain *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Iain Wilson iwilson@edv2.boku.ac.at *F Deparment fuer Chemie Tel \+43-1-36006-6065 *F Universitaet fuer Bodenkultur or \+43-664-310-1569 *F Muthgasse 18 or \+43-1-36006-6511 *F A-1190, Wien, Austria Fax \+43-1-36006-6059 *F Internet: http://www.boku.ac.at/chemie/bc1/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ # *U FBrf0179882 *a Hirota *b Y. *t 2004.11.8 *T personal communication to FlyBase *u Helping FlyBase: EDRC-J13. *F Date: Fri, 5 Nov 2004 15:55:56 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-J13 *F To: yhirota@sc.itc.keio.ac.jp *F Cc: rd120@gen.cam.ac.uk *F Dear Dr. Hirota, *F We are (belatedly) curating the abstracts for the upcoming 18th *F (Gottingen) European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'Identification of the gene involved in the differentiation of the *F postembryonic neuroblast'. *F You mention a gene symbol that is new to FlyBase, TN166. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 08 Nov 2004 21:04:56 \+0900 *F Subject: Re: Helping FlyBase: EDRC-J13 *F From: 'HIROTA, Yuki' *F To: 'Rachel Drysdale (Genetics)' *F Dear Dr. Rachel Drysdale, *F Thank you for your e-mail. The gene we called TN166 is corresponds to *F CG10203 (xl6). *F Best regards, *F Yuki Hirota *F HIROTA,Yuki, Ph.D. *F Department of Physiology *F Keio University School of Medicine *F 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. *F Phone 81-3-5363-3747 *F Fax 81-3-3357-5445 *F e-mail: yhirota@sc.itc.keio.ac.jp # *U FBrf0179883 *a Basler *b K. *t 2004.11.8 *T personal communication to FlyBase *u Helping FlyBase: EDRC-H13. *F Date: Fri, 5 Nov 2004 16:09:03 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-H13 *F To: basler@molbio.unizh.ch *F Cc: rd120@gen.cam.ac.uk *F Dear Dr. Basler, *F We are (belatedly) curating the abstracts for the 18th *F (Gottingen) European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F 'A Drosophila model of familial cylindromatosis'. *F You mention a gene symbol that is new to FlyBase, CYLD. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F With best regards, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Mon, 8 Nov 2004 14:41:26 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Konrad Basler *F Subject: Re: Helping FlyBase: EDRC-H13 *F Dear Rachel, *F it is CG5603 (= Drosophila homolog of CYLD). *F Thanks and best regards, *F Konrad # *U FBrf0179884 *a Roote *b J. *t 2004.11.9 *T personal communication to FlyBase *u GT000294 trivia. *F To: Kevin Cook , Kathy Matthews *F , Rachel Drysdale (Genetics) *F From: John Roote *F Subject: GT000294 trivia *F Date: Tue, 9 Nov 2004 09:35:04 \+0000 *F Hi Folks, *F As promised, GT000294 has been crossed to cdc2B47 and is indeed *F lethal. So (for Bloomers stock centre) the stock is OK and (for *F FlyBase) 294 should appear as an allele of cdc2 (in which it is *F inserted). *F Thank you *F John # *U FBrf0179885 *a Reim *b I. *t 2004.11.1 *T personal communication to FlyBase *u Df(3L)DocA etc molecular information. *F Date: Mon, 01 Nov 2004 18:24:17 \-0500 *F From: Ingolf Reim *F Subject: Df(3L)DocA etc molecular information *F To: flybase-updates@morgan.harvard.edu *F In Reim et al. (2003) Dev 130:3187-3204 we published *F the generation of 3 deficiencies (Fig. 5A), but *F exact sequences of breakpoints had not been included *F in the article. Here are the molecular data that *F characterize the breakpoints of the deficiencies *F reported. Data were obtained by sequencing an *F inverse PCR product from the 5' end of the retained *F P-insertion. The first 60 bases after the P-end are *F given (nucleotide 1 which is the first genomic *F nucleotide present after the last 5'EP nucleotide *F thus characterizes the 1st breakpoint). The *F unchanged 3'end of the EP insertion can be *F considered the other breakpoint. *F Sequence flanking EP(3)3556 in Df(3L)DocA: *F CGCTGCACAA TTTAAAATGC CAAATTAGAG TGACCGTCGC *F ACGAGGTCGC AAATATGCCA *F Sequence flanking EP(3)3556 in Df(3L)DocB: *F ACACAATTGA AACATGTCAC ATGCAGTGTG ACCGTTCTTG *F GCTCAATGGA AAAGCTCTCA *F Sequence flanking EP(3)584 in Df(3L)EP584MR2: *F GATACACCAA TGATTTACAA GGGCTGTCAA AGTTTTCAAT *F GCACTTAGGA GTGGTCACAC *F ______________________________________ *F Ingolf Reim, Ph.D. *F Brookdale Department of *F Molecular, Cell and Developmental Biology *F Mount Sinai School of Medicine *F One Gustave L. Levy Place, Box 1020 *F New York, NY 10029 *F USA *F phone (212)-241 0105 *F ingolf.reim@mssm.edu # *U FBrf0179886 *a Mason *b J. *t 2004.11.26 *T personal communication to FlyBase *u Miniwhite in BSC deletions. *F Date: Tue, 26 Oct 2004 15:30:03 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: miniwhite in BSC deletions *F The following information was provided to the Bloomington Stock Center by *F Jim Mason, NIEHS (10/04). *F Df(3L)BSC8 (FBab0029684) *F The miniwhite marker from P{lacW}l(3)j11B2j11B2 was deleted or disrupted. *F Df(3L)BSC10 (FBab0029692) *F The miniwhite markers from both P{lacW}mirrcre2 and P{lacW}RpS12s2783 *F were deleted or disrupted. *F Df(2R)BSC11 (FBab0029712) *F The miniwhite marker from P{lacW}l(2)s3475s3475 was deleted or disrupted. *F Df(3L)BSC13 (FBab0029723) *F The miniwhite markers from both P{lacW}Nmtj1C7 and P{EP}ergic53EP3212 *F were deleted or disrupted. *F Df(2L)BSC16 (FBab0029789) *F The miniwhite marker from P{lacW}exk12193 was deleted or disrupted. *F Df(2L)BSC17 (FBab0029725) *F The miniwhite marker from P{EP}DrefEP460 was deleted or disrupted. *F Df(2R)BSC18 (FBab0029741) *F The miniwhite markers from both P{lacW}mamk02214 and P{EP}EP993 were *F deleted or disrupted. *F Df(2R)BSC19 (FBab0029795) *F The miniwhite markers from both P{EP}EP951 and P{lacW}l(2)s4831s4831 were *F deleted or disrupted. *F Df(2R)BSC22 (FBab0029834) *F The miniwhite marker from P{EP}betaTub56DEP2640 was deleted or disrupted. *F Df(3L)BSC23 (FBab0029837) *F The deficiency chromosome retains the miniwhite marker from *F P{lacW}dosP115 and/or P{lacW}Hsp83j5C2. *F Df(3L)BSC27 (FBab0029948) *F The miniwhite marker from P{lacW}l(3)L4060L4060 was deleted or disrupted. *F Df(2L)BSC28 (FBab0029867) *F The deficiency chromosome retains the miniwhite marker from P{EP}EP2297. *F Df(2R)BSC29 (FBab0029979) *F The miniwhite marker from P{lacW}l(2)k09501k09501 was deleted or disrupted. *F Df(3L)BSC33 (FBab0029990) *F The deficiency chromosome retains the miniwhite marker from *F P{lacW}Cdc27L7123 and/or P{lacW}l(3)j1D5j1D5. *F Df(2L)BSC37 (FBab0037634) *F The deficiency chromosome retains the miniwhite marker from P{EP}dppEP2232. *F Df(3R)BSC38 (FBab0036258) *F The deficiency chromosome retains the miniwhite marker from P{EP}EP3681 *F and/or P{EP}EP3340EP3340. *F Df(2R)BSC39 (FBab0037639) *F The miniwhite marker from P{GT1}Rep1BG01033 was deleted or disrupted. *F Df(2R)BSC40 (FBab0037640) The deficiency chromosome retains the miniwhite *F marker from P{GT1}Rep1BG01033. *F Df(3R)BSC42 (FBab0037759) *F The miniwhite marker from P{EP}EP3088 was deleted or disrupted. *F Df(3R)BSC43 (FBab0037760) *F The deficiency chromosome retains the miniwhite marker from *F P{lacW}Rab11j2D1. *F Df(2R)BSC44 (FBab0037744) *F The deficiency chromosome retains the miniwhite marker from *F P{lacW}l(2)k04222bk04222b and/or P{EP}CG14478EP2283. *F Df(2R)BSC45 (FBab0037745) *F The deficiency chromosome retains the miniwhite marker from *F P{lacW}l(2)k07406k07406 and/or P{SUPor-P}CG6424KG00595. *F Df(3L)BSC46 (FBab0037753) *F The miniwhite marker from P{EP}EP771 was deleted or disrupted. *F Df(3R)BSC47 (FBab0037761) *F The miniwhite markers from both P{EP}CG31549EP3625 and *F P{EP}CG2017EP3503 were deleted or disrupted. *F Df(3R)BSC48 (FBab0037762) *F The miniwhite marker from P{EP}EP3281 was deleted or disrupted. *F Df(2R)BSC49 (FBab0037746) *F The deficiency chromosome retains the miniwhite marker from P{EP}EP2344 *F and/or P{EP}CG14478EP2283. *F Df(2L)BSC50 (FBab0037839) *F The deficiency chromosome retains the miniwhite marker from *F P{EP}CG13130EP2238 and/or P{EPgy2}EY03684. *F Df(2L)BSC51 (FBab0037840) *F The miniwhite markers from both P{GT1}CG15636BG01429 and P{GT1}BG01694 *F were deleted or disrupted. *F Df(2L)BSC52 (FBab0037841) *F The deficiency chromosome retains the miniwhite marker from *F P{GT1}CG15636BG01429. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179887 *a Beitel *b G. *t 2004.11.4 *T personal communication to FlyBase *u *F Date: Thu, 04 Nov 2004 15:38:36 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: w- version of CyO, P{Dfd-EYFP}2 *F The following information was provided to the Bloomington Stock Center by *F Greg Beitel, Northwestern University (10/04) to describe the isolation of a *F w- derivative of CyO, P{w+mC=Dfd-EYFP}2. *F 'Information for CyO dfd-eYFP w- *F Constructed by: Greg Beitel, Matthew Slovitt, ZhiGuo Liang, Stephanie Ray, *F Marcus Yu, Heeren Patel *F This is a 'w-,' 'direct drive' (i.e. not Gal4/UAS) fluorescent balancer *F with strong eYFP expression in the head region visible from stage late 14 *F and continuing through adulthood. In late embryos and larva there is *F expression in the posterior spiracles and to some extent the denticle *F belts. The YFP is easily scorable by compound or dissecting *F microscope. In adults the proboscis is scorable. The eYFP can be stained *F immunohistochemically using a standard 25 min 4% formaldehyde fix and a *F the 3E6 mouse anti-GFP monoclonal (Molecular Probes). This balancer *F chromosome is 'w-' because the w+ transformation marker was inactivated by *F a gamma ray induced mutation that does not seem to significantly affect *F YFP expression. The nature of the mutation that inactivates the w+ is not *F know. Alternative w- revertant strains are available from G. Beitel if *F this isolate has collateral damage that deletes your favorite gene as well *F as the w+. *F The dfd-HIYFP transposon was made using the autoregulatory element of the *F deformed promotor and contains the 2.7 kb genomic Xba fragment named 'Hz2.7 *F REV' that had been cloned by Bergson and McGinnis (EMBO vol. 9 1990) into *F pBluescript. The Hz2.7REV fragment was excised using NotI/BamHI and *F dropped into the NotI/BamHI sites of the pYellow HI-Pelican vector (Beitel *F unpublished ; Genbank accession number AY730637) which is derived from the *F pGreen H Pelican vector of Barolo et al., Biotechniques vol. 29, *F 2000). The pYellow HI-Pelican contains eYFP (Clontech) rather than the *F original eGFP, and a small intron was added between the transcription *F start site and the eYFP coding sequence. The intron was added to test if *F splicing would improve expression, but this intron does not appear to *F significantly improve expression compared to an intronless *F construct (G. Beitel unpublished). The original isolation name of this *F line was dfd::HIYFP4_MWS5.' *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0179888 *a Ryder *b E. *c J. *d Roote *t 2004.11.10 *T personal communication to FlyBase *u DrosDel deletions confirmed by PCR and genetic tests. *F Date: Wed, 10 Nov 2004 11:24:23 \+0000 *F From: Ed Ryder *F To: 'Rachel Drysdale (Genetics)' *F Subject: DrosDel deletions confirmed by PCR and genetic tests. *F Hi Rachel, *F The following DrosDel deletions have been confirmed by PCR (using the *F 3-step process described on the web site) and by genetic *F complementation (see following mail from Roote) against known mutations *F on the Release3.1 sequence. *F Df(2L)ED3 *F Df(2R)ED1552 *F Df(2R)ED1 *F Df(3L)ED4177 *F Df(3R)ED5020 *F Df(3R)ED5021 *F Df(3R)ED5071 *F Df(3R)ED5095 *F Df(3R)ED5100 *F Df(3R)ED5138 *F Df(3R)ED5142 *F Df(3R)ED5147 *F Df(3R)ED5156 *F Df(3R)ED5177 *F Df(3R)ED5196 *F Df(3R)ED5223 *F Df(3R)ED5230 *F Df(3R)ED5296 *F Df(3R)ED5429 *F Df(3R)ED5516 *F Df(3R)ED5558 *F Df(3R)ED5559 *F Df(3R)ED5608 *F Df(3R)ED5622 *F Df(3R)ED5642 *F Df(3R)ED5705 *F Df(3R)ED5780 *F Df(3R)ED2 *F Df(3R)ED5911 *F Df(3R)ED5938 *F Df(3R)ED6025 *F Df(3R)ED6076 *F Df(3R)ED6085 *F Df(3R)ED6093 *F Df(3R)ED6096 *F Df(3R)ED6103 *F Df(3R)ED6187 *F Df(3R)ED6220 *F Df(3R)ED6232 *F Df(3R)ED6235 *F Df(3R)ED6242 *F Df(3R)ED6255 *F Df(3R)ED6310 *F Df(3R)ED6316 *F Df(4)ED6364 *F Df(4)ED6366 *F Df(4)ED6382 *F Df(4)ED6384 *F Df(1)ED7374 *F Cheers, *F Ed *F \-- *F ===================================== *F Dr. Ed Ryder *F Department of Genetics, University of Cambridge, *F Downing Street, Cambridge, CB2 3EH *F Tel: \+44 (0)1223 765928. *F Email: e.ryder@gen.cam.ac.uk *F http://www.drosdel.org.uk *F ===================================== *F To: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: ED genetic confirmation *F Date: Thu, 11 Nov 2004 20:10:44 \+0000 *F Rachel, *F Complementation crosses with EDs. *F Df(2R)ED1552 (from Ed's list) not confirmed genetically. *F John *F 'Df name','non-complementing','complementing (viable, no obvious phenotype)' *F 'Df(2L)ED3','osp, noc (weak), gft, nht','el, esg' *F 'Df(2R)ED1','RhoGEF2, Df(2R)P803-D15','l(2)k10815, Gst2, l(2)BG00665, *F l(2)k08805' *F 'Df(3L)ED4177','emc', *F 'Df(3R)ED5020','hkb, tub', *F 'Df(3R)ED5021','hkb, tub', *F 'Df(3R)ED5071','opa, Karybeta3, tub', *F 'Df(3R)ED5095','Karybeta3, opa', *F 'Df(3R)ED5100','Karybeta3, tub, corto07128b (semi-lethal)', *F 'Df(3R)ED5138','opa, corto', *F 'Df(3R)ED5142','opa, corto, Karybeta3', *F 'Df(3R)ED5147','cno, Hph', *F 'Df(3R)ED5156','Hph, kkv','cno' *F 'Df(3R)ED5177','Atu','Rga03834 (reported by Sprad to be a semi-lethal). *F elF-5C (ED5177 predicted to delete only 540bp of gene)' *F 'Df(3R)ED5196','cas', *F 'Df(3R)ED5223','dsx, Tom34', *F 'Df(3R)ED5230','Tom34, stck', *F 'Df(3R)ED5296','Mcm2', *F 'Df(3R)ED5429','Scm, dmt', *F 'Df(3R)ED5516','pros','RpL3KG05440' *F 'Df(3R)ED5558','aur, svp, sad', *F 'Df(3R)ED5559','aur, sad', *F 'Df(3R)ED5608','Df(3R)Exel7318','Df(3R)kar-H5' *F 'Df(3R)ED5622','ems, flflL4179 (unverified lethal: over ED5622 wings *F held-down, veins disrupted, low female fertility.)', *F 'Df(3R)ED5642','trx, ems, flflL4179 (unverified lethal: over ED5622 wings *F held-down, veins disrupted, low female fertility.)', *F 'Df(3R)ED5705','Atx2', *F 'Df(3R)ED5780','Df(3R)ED5794','Dadj1e4 and Sur-8e02803, unverified lethals' *F 'Df(3R)ED2','fray07551, Df(3R)Cha-9, l(3)S007302 (unverified lethal), *F Df(3R)Dl-M2','l(3)02515 and l(3)S148213 unverified lethals, gl' *F 'Df(3R)ED5911','cdi, sqz, nos', *F 'Df(3R)ED5938','sqz', *F 'Df(3R)ED6025','GluCalpha, bnl', *F 'Df(3R)ED6076','tsl, E2f, how', *F 'Df(3R)ED6085','how, sar1, tld', *F 'Df(3R)ED6093','sar1, Dph5', *F 'Df(3R)ED6096','hh, cnc, Df(3R)ED6091', *F 'Df(3R)ED6103','hh, cnc', *F 'Df(3R)ED6187','Apc2, crib', *F 'Df(3R)ED6220','CG11836f02631 (unverified lethal), tld', *F 'Df(3R)ED6232','gro', *F 'Df(3R)ED6235','scrib','gro' *F 'Df(3R)ED6242','pll', *F 'Df(3R)ED6255','pll', *F 'Df(3R)ED6310','stg','Ef1gammae00972 (unverified lethal)' *F 'Df(3R)ED6316','kay, Dr, stg, Df(3R)ED6310', *F 'Df(4)ED6364','RpS3A (note: stock is phenotypically Minute), panciD, gvl','bt' *F 'Df(4)ED6366','panciD, gvl','bt' *F 'Df(4)ED6382','lgs, eyD, bt','pan, ci, gvl' *F 'Df(4)ED6384','eyD, bt','ci, gvl' *F 'Df(1)ED7374','if', # *U FBrf0182544 *a Baker *b N. *t 2005.1.6 *T personal communication to FlyBase *u *F Date: Thu, 6 Jan 2005 16:29:58 \-0800 *F To: flybase-help@morgan.harvard.edu *F From: 'Dr. Nicholas Baker' *F Subject: wgCX1 *F Dear Flybase, *F Although the so-called allele wgCX1 was isolated by failure *F to complement wg1 (see EMBO J 6:1765-1773), the phenotype *F associated with wgCX1/wg1 is weak and poorly penetrant, and *F wgCX1 fully complements amorphic wg alleles. These data make *F allelism to wg uncertain. Since the so-called wgCX1 chromosome *F contains a deletion that uncovers arrow (Df(2R)CX1, 49C1-4;50C23-D2 *F see Hu et al Genetics 141:607-617), an alternative explanation of *F intergenic non-complementation between arrow and wg1 is plausible. *F sincerely *F Nick Baker *F \-- *F Nicholas E. Baker PhD *F Professor of Molecular Genetics *F Department of Molecular Genetics *F Albert Einstein College of Medicine *F 1300 Morris Park Avenue *F Bronx *F NY 10461 *F USA *F Tel. (+01) 718-430-2854 *F Fax. (+01) 718-430-8778 *F http://fruitfly4.aecom.yu.edu/index.html # *U FBrf0182566 *a Beitel *b G. *t 2005.3.25 *T personal communication to FlyBase *u P{Dfd-EYFP}3 insertion. *F Date: Fri, 25 Mar 2005 15:34:22 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{Dfd-EYFP}3 insertion *F The following information accompanied a stock donated to the Bloomington *F Stock Center by Greg Beitel, Northwestern University (2/05). *F Greg Beitel, Tien Le, Heeren Patel and Marcus Yu isolated an insertion of *F P{Dfd-EYFP} in the balancer variant TM6B-Sb1 (FBba0000282) called *F P{Dfd-EYFP}3. The balancer short name to be used at Bloomington is TM6B, *F P{w+mC=Dfd-EYFP}3, Sb1 Tb1 ca1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182567 *a Bejsovec *b A. *t 2005.1 *T personal communication to FlyBase *u RacGap50C mutations. *F Date: Fri, 04 Mar 2005 15:22:47 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: RacGap50C mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Amy Bejsovec of Duke University, (1/05). *F RacGap50CAR2 and RacGap50CDH15 are nonsense mutations in RacGap50C *F (FBgn0033881). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182569 *a Bellen *b H. *t 2005 *T personal communication to FlyBase *u *F Date: Fri, 21 Jan 2005 09:44:23 \-0600 *F To: David Sutherland , jskeath@genetics.wustl.edu *F From: Hugo Bellen *F Subject: Re: spdo/tmod split *F Hi, *F At 02:26 PM 1/21/2005 \+0000, you wrote: *F >Dear Hugo and James, *F > *F >I'm currently engaged in the tricky task of trying to split FlyBase's current *F >record for spdo into 2 (tmod/CG1539 & spdo/CG31020), based on the data in *F >'O'Connor-Giles and Skeath, 2003, Dev. Cell 5(2): 231--243'. *F > *F >I'd like to check with you that my proposed partitioning of the alleles fits *F >with your understanding of their behaviour. Any additional *F >complementation or *F >molecular data that could help with this partitioning would be most *F >welcome. I *F >can curate such data as a personal communication to FlyBase. *F > *F >The tough question is what to do with 'spdoH7'(*) and its associated data. *F > *F > On the basis of the mapped insertion 'P{lacW}spdoH7' (now to be renamed *F >P{lacW}tmod)H7) it needs to be an allele of tmod. On the basis of *F >failure to *F >complement spdoC55 (see below), it needs to be an allele of spdo. The most *F >obvious explanation is that the 'H7' chromosome has mutations in both genes \- *F >suggesting I should make both alleles. This still leaves the problem of *F >where *F >to put the phenotypic data. *F It most likely hits two or more genes. As far as I can establish, we do *F not know what the phenotype is of tmod, but Jim can prove me wrong. *F >Any suggestions? *F > *F >Here is how I plan to split the rest of the alleles between the two genes: *F > *F >Alleles remaining spdo/CG31020: *F > *F >1. leisions mapped by O'Conner-Giles & Skeath: *F > *F >spdoC55 *F >spdoG104 *F >spdoK433 *F > *F >spdoZZ27 (a deficiency removing spdo/CG31020 : data from FBrf0162056 == *F >O'Connor-Giles and Skeath, 2003, Dev. Cell) *F > Strictly we only make alleles from Dfs when one of their breakpoints is *F > in the *F >gene in question \- Is this the case? *F spdoZZ27 is a deletion that removes both tmod and spdo, and other genes. It *F is a null for tmod (no transcript) *F >2. On the basis of complementation: *F > *F >spdoS097002b *F >Separable from: @P{lacW}S097002a@ *F >data from: FBrf0099763 Salzberg et al., 1997: *F >Fails to complement: spdoC55 *F >Southern analysis reveals a chromosomal rearrangement. *F >Lethality not revertible by &Dgr;2-3-induced mobilization *F > *F >Fails to complement: spdoC55 *F >Isolation of @spdoH7@ by failure to complement @spdoC55@ is described in *F >'Dye et al., '98 FBrf0102830' *F > *F > *F >Alleles to split out as tmod: *F >1. constructs: *F >spdohs.PDL is the only one of these *F >'rescue' data for 'spdoH7' \- should this now be recorded as partial *F >suppression of a spdoH7 phenotype by hs-tmod? *F That is the best explanation as the partial rescue in the PNS was repeated *F blind. *F >2. Insertions mapped to CG1539: *F > *F >spdo02288 *F >spdo00848 *F > *F >3. lesions mapped to CG1539 *F > *F >spdoS130910 *F >A portion of the CG1539 gene is deleted. (data Dye et al., '98 FBrf0102830) *F Correct *F >spdoZZ27 (a deficiency removing tmod/CG1539: data from FBrf0162056 == *F >O'Connor-Giles and Skeath, 2003, Dev. Cell) *F > Strictly we only make alleles from Dfs when one of their breakpoints is *F > in the *F >gene in question \- Is this the case? *F Jim may know the precise breakpoint.. *F > *F >4. On basis of complementation only: *F > *F >spdorG347 *F >Fails to complement: spdo00848 *F >(data from BDGP) *F > *F > *F >Thanks in advance *F Sorry for the confusion, but double hits with P-elements, the deletion *F associated with zz27, and the partial rescue, are at the base of much of *F the problems. *F Please confer with Jim, but I think you got most of it... *F Hugo # *U FBrf0182572 *a Ben-Shahar *b Y. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50708. *F Date: Wed, 2 Mar 2005 13:30:15 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50708 *F To: yehuda-ben-shahar@uiowa.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Yehuda, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A possible role for a DEG/ENaC ion channel in courtship behavior. *F You mention a gene symbol that is new to FlyBase, ppk25. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. *F With best wishes, *F Rachel. *F Subject: RE: Helping FlyBase: ADRC-50708 *F Date: Wed, 2 Mar 2005 13:51:56 \-0600 *F From: 'Ben Shahar, Yehuda' *F To: 'Rachel Drysdale \(Genetics\)' *F Hi Rachel, *F ppk25 is synonymous with CG33349. I should have mentioned it in the *F abstract. *F Thanks. *F Yehuda Ben-Shahar, PhD *F Howard Hughes Medical Institute, 500 EMRB *F University of Iowa, College of Medicine *F Iowa City, IA 52242, U.S.A. *F Tel: (319) 335-7574 *F Fax: (319) 335-7623 *F email: yehuda-ben-shahar@uiowa.edu # *U FBrf0182581 *a Berryman *b M. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50093. *F Date: Wed, 2 Mar 2005 13:42:14 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50093 *F To: berryman@ohiou.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Mark, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Drosophila CLIC-like gene is critical for viability during *F development, adult lifespan and resistance to oxidative stress. *F You mention a gene symbol that is new to FlyBase, Clic. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F From: 'Berryman, Mark' *F To: 'Rachel Drysdale (Genetics)' *F Subject: RE: Helping FlyBase: ADRC-50093 *F Date: Wed, 2 Mar 2005 11:23:59 \-0500 *F Dear Rachel: Our gene, which we tentatively refer to as 'DCLIC', *F corresponds to CG10997. The acronym CLIC stands for chloride intracellular *F channel. There are 6 CLIC genes in humans, and apparently, only 1 CLIC-like *F gene in Drosophila. Please let me know if you have any more questions. *F Mark Berryman # *U FBrf0182594 *a Bloomington Drosophila Stock Center *b ?. *t 2005.2.3 *T personal communication to FlyBase *u Df(2L)Exel9043. *F From: Kathy Matthews *F Date: Thu, 3 Feb 2005 09:53:53 \-0500 (EST) *F Subject: Df(2L)Exel9043 *F Personal communication from: Bloomington Drosophila Stock Center *F The FlyBase entry: *F http://flybase.bio.indiana.edu/.bin/fbidq.html?FBab0038083 *F needs correcting to be Df(2L) instead of Df(2R). *F The insertions used for the Df are PBac{RB}e02285 (minus orientation) *F and P{XP}d05357 (minus) at 19415971 and 19430995, respectively, on 2L. *F This corresponds to 37E1;37E1. *F (The original Excel table of Exel Dfs incorrectly identified the arm of *F the insertions used to make this Df. This propagated errors to cytology *F predictions, etc.) *F Our complementation tests show it fails to complement Df(2L)Exel7075, *F Df(2L)VA17 and Df(2L)E55. # *U FBrf0182632 *a Carrera *b I. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50283. *F Date: Wed, 2 Mar 2005 13:48:47 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50283 *F To: carrei01@med.nyu.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Ines, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F In vivo role of the PBAP chromatin remodeling complex in Drosophila. *F You mention a gene, Bap170, for which we already have a record in *F FlyBase, and a new gene, baf180. Do you know which of the Genome *F Project CG annotations your genes correspond to? All the CGs have *F corresponding gene records in FlyBase already and we don't like to make *F duplicate records for what is actually the same gene unless we can't *F avoid it. The CG symbols become synonyms when an annotation is named *F with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Wed, 02 Mar 2005 19:01:45 \-0500 (EST) *F From: Ines Carrera *F Subject: Re: Helping FlyBase: ADRC-50283 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F We are refering to the CGs as described in the following paper: *F Differential targeting of two distinct SWI/SNF-related Drosophila *F chromatin-remodeling complexes. Mohrmann L, Langenberg K, Krijgsveld J, *F Kal AJ, Heck AJ, Verrijzer CP. *F bap170 corresponds in the paper to CG3274 and baf180 to CG11375 this last *F one is the homolog to the already described baf180 gene in humans. *F I hope this was helpful, please let me know if you need any further *F clarification. *F Thanks!, *F Ines # *U FBrf0182639 *a Celniker *b S. *t 2005.3.1 *T personal communication to FlyBase *u *F Date: Tue, 1 Mar 2005 22:25:38 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: Gene data (problem or question) *F comments: According to the gene record for 'tungus' CG30084, the *F annotation of the transcripts RA, RB, RC and RD is based on the BDGP EST and *F cDNA evidence. However I cannot find a transcript model corresponding to the *F only apparently full-length completed cDNA sequence RH03424. Please add a *F gene model corresponding to RH03424. Please review the previous gene models *F as I am not sure they are correct. *F Thanks Sue *F realname: Susan Celniker *F reply-to: celniker@fruitfly.org *F source: FB-NG Help Mail: # *U FBrf0182644 *a Chandrashekaran *b S. *t 2005.1.4 *T personal communication to FlyBase *u *F Date: Tue, 4 Jan 2005 23:58:01 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: Gene data (problem or question) *F comments: The map position of stambh A has been corrected to 44D1-2 (Kumar *F et al, 2001, Journal of Genetics. 80:83-95). This may please be corrected in *F FLYBASE records. stambhA was discovered by our group 20 years back in *F 1985(Shyngle and sharma, Indian J of Exptl Biol. 23:235-240 during a search *F for ts paralytic mutants. *F Based upon alleles taken from us the gene has been recently been *F identified to be a lipase involved in phototransduction (Huang et al, 2004. *F Nature Neuroscience,7: 1070-1078). Huang et al have however renamed stambh A *F as `Rolling blackout', which is not warranted. stambh is a Sanskrit word *F meaning `cessation in general and of locomotion in particular '. This word *F describes the properties of the temperature sensitive paralysis and *F temperature sensitive phototransduction properties of the mutant very aptly *F and should not be changed .It is unreasonable to rename genes based upon *F restricted facets of their function. It is appropriate to mention here that *F the gene `wingless' also was discovered in our laboratory in New Delhi, India *F in 1976(Sharma, R.P and Chopra V.L). However its name is retained eventhough *F it has myriad phenotypes other than 'winglessness'. I trust that FLYBASE will *F agree with me over the use of stambh A in preference to Rolling blackout. *F Shanti Chandrashekaran, *F I.C.A.R. National Fellow, *F Division of Genetics, *F Indian Agricultural Research Institute *F New Delhi 110012. *F source: FB-NG Help Mail: *F Sent from computer 203.197.227.228 # *U FBrf0182652 *a Chen *b J. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50611. *F Date: Wed, 2 Mar 2005 13:27:09 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50611 *F To: jian.chen@pharma.novartis.com *F Cc: rd120@gen.cam.ac.uk *F Dear Jian, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Mitochondrial Biogenesis in Drosophila S2 Cells *F You mention a gene symbol that is new to FlyBase, NRF-1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50611 *F From: jian.chen@pharma.novartis.com *F Date: Wed, 2 Mar 2005 10:43:40 \-0500 *F Dear Rachel, *F Actually NRF is ewg (erect wing), CG3114. I am sorry for the inconvience *F that brings to you. NRF is the name of its mammalian counterpart. *F Best, *F Jenny *F Jian (Jenny) Chen, PH.D *F NIBRI, Novartis INstitute for Biomedical Research *F 250 Massachusettes Ave, 5C-460 *F Cambridge, MA 02139 *F Tel: 617-871-7411 *F Fax: 617-871-4071 # *U FBrf0182658 *a Cheney *b C. *t 2005.3.4 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50825. *F Date: Wed, 2 Mar 2005 13:33:59 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50825 *F To: cmc04747@pomona.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Naveen, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F You mention a gene symbol that is new to FlyBase, Gint3. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Fri, 4 Mar 2005 09:47:04 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: 'Clarissa M. Cheney' *F Subject: Re: Helping FlyBase: ADRC-50825 *F Rachel--it's CG5469. *F Cris Cheney # *U FBrf0182663 *a Cho *b K.O. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50952. *F Date: Wed, 2 Mar 2005 13:40:55 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50952 *F To: kcho@bcm.tmc.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Kyung-Ok, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A novel Dlg-interacting metalloprotease and Strabismus regulate cell *F growth by modulating the level of Discs Large. *F You mention a gene symbol that is new to FlyBase, Dimp. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Fri, 1 Jan 1904 12:42:46 \-0600 *F To: 'Rachel Drysdale (Genetics)' *F From: Kyung-Ok Cho *F Subject: Re: Helping FlyBase: ADRC-50952 *F Dear Rachel, *F I was going to report this gene to Flybase after its publication. *F Since you are asking about it, I want to tell you that the dimp gene *F is incorrectly annotated as two separate genes, CG11169 and CG9850 in *F Flybase. I isolated multiple dimp cDNAs spanning both CGs. If you *F have further questions, I will be happy to provide more information. *F Sincerely, *F Kyung # *U FBrf0182682 *a Cook *b K. *t 2004.12.14 *T personal communication to FlyBase *u *F Date: Tue, 14 Dec 2004 13:38:50 \-0500 *F To: 'Rachel Drysdale (Genetics)' , *F matthewk@fly.bio.indiana.edu *F From: Kevin Cook *F Subject: Re: new balancers *F Cc: rd120@gen.cam.ac.uk *F Hi Rachel-- *F \*I CyO-KG00000 *F \*U CyO, P{y+mDint2 wBR.E.BR=SUPor-P}KG00000 *F is really the same thing as *F CyO, P{y+mDint2 wBR.E.BR=SUPor-P}RR1 *F described under the FBti0012145 and FBba0000396 entries. When I was in *F Gary Karpen's lab, I got the CyO chromosome with the P{SUPor-P} insertion *F from Pam Geyer's lab, where Robin Roseman (the 'RR' of 'RR1') isolated the *F insertion. Gary sent the stock to Hugo Bellen's lab where it was used in *F FBrf0179132. It's the same CyO insertion of P{SUPor-P} that was used as *F the P source in the following two papers, too: *F Dobie, K.W., Kennedy, C.D., Velasco, V.M., McGrath, T.L., Weko, J., *F Patterson, R.W., Karpen, G.H. *F Identification of chromosome inheritance modifiers in Drosophila melanogaster. *F Genetics 2001 157(4):1623--1637 *F FBrf0135802 *F Yan CM, Dobie KW, Le HD, Konev AY, Karpen GH. *F Efficient recovery of centric heterochromatin P-element insertions in *F Drosophila melanogaster. *F Genetics 2002 161(1):217--229 *F FBrf0149015 *F So, it seems to me that P{SUPor-P}KG00000 should be listed as a synonym for *F P{SUPor-P}RR1. *F Muchos gracias, *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0182691 *a Craig *b C. *t 2003.12.16 *T personal communication to FlyBase *u p38c gene. *F Date: Tue, 16 Dec 2003 16:03:31 \-0600 *F To: flybase-updates@morgan.harvard.edu *F From: Callie Craig *F Subject: p38c gene *F I am writing to express the opinion that the predicted gene p38c at 91E1-2 *F is not a third Drosophila p38/MAPK gene. This opinion is based on three *F observations. While the proximity and overall sequence homology of p38c to *F D-p38a does suggest a recent genomic duplication, two key changes in the *F genomic sequence make it impossible for this gene to code a functional *F MAPK. The most important of these is that the invariant p38 MAPK dual *F phosphorylation motif, TGY, has been changed to TDH in p38c (AA 177-179). *F This motif cannot serve as a substrate for dual phosphorylation. (The *F phosphorylation motifs for ERK and JNK are TEY and TPY, respectively.) *F Second, the 8 amino acid catalytic loop in p38c contains two changes, *F likely to render it nonfunctional (AA 144-151). Lastly, to date no ESTs *F have been found for this gene. *F Callie Craig *F Cagan Lab *F Department of Molecular Biology & Pharmacology *F Washington University School of Medicine *F 660 S. Euclid, Box 8103 *F St. Louis, MO 63110 *F Tel: 314-362-7797 *F FAX: 314-362-7058 # *U FBrf0182698 *a Curtin *b K. *c S. *d Wasserman *e B. *f Reed *t 2005.2.11 *T personal communication to FlyBase *u gelded naming. *F Date: Fri, 11 Feb 2005 16:08:49 \-0600 *F From: Kathy Curtin *F Subject: Fwd: Re: gelded naming *F To: matthewk@indiana.edu *F Kathy Matthews: *F Below is a message from Steve Wasserman indicating that he feels it makes *F more sense for his original allele of gel to be a basigin allele than the *F other way around. This rationale is based on the fact that a transcript *F and protein have been shown to be basigin. He is in favor of changing the *F name and for retaining gel as the designation of an allele for basigin. *F Kathy Curtin *F >Date: Fri, 11 Feb 2005 14:01:53 \-0800 *F >From: Steven Wasserman *F >Subject: Re: gelded naming *F >To: Kathy Curtin *F >Cc: Kathy Matthews *F > *F >Kathy \- *F > *F >I think the reasons for sticking with historical names are solid, but not *F >in all cases. It is not as if there were a lot of work that would be *F >thrown into confusion. In some ways I prefer the opposite solution to what *F >you propose. Faint little balls and Torpedo are now alleles of EGFR, not *F >the other way around. Gelded is a mutation, not a gene, and Basigin is a *F >gene, not a mutation. However, whatever causes you the least problems now *F >and for the future sounds like a good solution to me. *F > *F >- Steve *F Date: Mon, 14 Feb 2005 14:04:37 \-0800 *F To: matthewk@fly.bio.indiana.edu, matthewk@indiana.edu *F From: reed@resultra.sickkids.on.ca *F Subject: CG31605/basigin/gelded *F Cc: kcurtin@mail.uark.edu, stevenw@ucsd.edu, *F lipshitz@sickkids.ca *F Dear Kathy, *F This is just a brief note to let you know that I support Kathy Curtin's *F suggestion of designating CG31605 as basigin (symbol: bsg) and not gelded *F (symbol: gel). I believe the genetic arguments have been laid out by Kathy *F (Df removing CG31605 complements gel1), and that Steve Wasserman has also *F indicated that the assignment of gelded as the transcript associated with *F CG31605 (by the genome project) is questionable. I have found that various *F P element insertions in the vicinity of CG31605 show complex *F geneticcomplementation. Some of my comments already appear as a *F personal communication to flybase in the listing for gel. If future *F fine structure mapping of this locus proves gelded to, in fact, be a male *F sterile allele of CG31605, perhaps the name gelded could be retained as an *F allele designation (gel1 = bsggelded? *F As Kathy points out, and to which I agree, the literature on *F basigin is certainly already bogged down by several different names and *F it would be a shame for the Drosophila community to add yet another *F complication by referring to the gene as 'gelded or CG31605' but the *F protein as 'D-basigin'. *F sincerely, *F Bruce Reed # *U FBrf0182709 *a David *b N. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50458. *F Envelope-to: rd120@gen.cam.ac.uk *F Delivery-date: Wed, 2 Mar 2005 13:20:50 \+0000 *F Date: Wed, 2 Mar 2005 13:20:47 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50458 *F To: ndavid@curie.fr *F Cc: rd120@gen.cam.ac.uk *F Dear Nicolas, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Spindle orientation during asymmetric division of the sensory organ precursor *F cell. *F You mention a gene symbol that is apparently new to FlyBase, RIC-8. *F Can youtell us what RIC stands for? Also do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. The CG symbols become synonyms when an annotation is *F named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Wed, 02 Mar 2005 15:26:19 \+0100 *F From: Nicolas DAVID *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50458 *F Dear Rachel, *F We refer to our gene as ric8 (we suppressed the '-') in reference to the name *F used for its homolog in C.elegans (Afshar K. et al. Cell 119, 2004; *F Couwenbergs C. et al., Current Biology, 14, 2004). Ric-8 is also known as *F synembryn. *F In Drosophila, we found two CG with good homology to C.elegans ric-8. *F They are CG15797 (gene l(1)G0397) and CG15910. So far we decided to refer to *F CG15797 as ric8a and CG15910 as ric8b, eventhough CG15910 is likely to be a *F pseudogene. *F So far, none of this is published, and I know other groups are working on the *F same genes. I don't know what they have decided regarding gene names, although *F I think they will refer to CG15797 as ric8. *F I hope this answers your question. Please feel free to ask for more details if *F you need. *F With best wishes, *F Nicolas # *U FBrf0182715 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC104. *F Date: Thu, 10 Mar 2005 11:37:59 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC104 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC104 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC104 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f05491 and P{XP}d03256. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f05491/ P{XP}d03256 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC104 from the segment *F of PBac{WH}f05491 to the left of its FRT site and the segment of *F P{XP}d03256 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f05491 to be at Release 3 genomic *F coordinate 21374074 on chromosome arm 2L and the insertion site of *F P{XP}d03256 to be at Release 3 genomic coordinate 21406302 on arm 2L. The *F Gene Disruption project determined the insertion site of PBac{WH}f05491 to *F be at Release 3 genomic coordinate 21373577 on arm 2L and the insertion *F site of P{XP}d03256 to be at Release 3 genomic coordinate 21405943 on arm *F 2L. The cytological breakpoints of Df(2L)BSC104 predicted from these *F coordinates are 39D5;39E2. It failed to complement Df(2L)Exel7081. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182716 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC105. *F Date: Thu, 10 Mar 2005 11:38:03 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC105 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC105 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC105 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f06874 and P{XP}d05609. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f06874/P{XP}d05609 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC105 from the segment *F of PBac{WH}f06874 to the left of its FRT site and the segment of *F P{XP}d05609 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f06874 to be at Release 3 genomic *F coordinate 20821521 on chromosome arm 2L and the insertion site of *F P{XP}d05609 to be at Release 3 genomic coordinate 20860125 on arm 2L. The *F Gene Disruption project determined the insertion site of PBac{WH}f06874 to *F be at Release 3 genomic coordinate 20821642 on arm 2L. The cytological *F breakpoints of Df(2L)BSC105 predicted from these coordinates are *F 38F2;38F4. It failed to P{SUPor-P}CG9339KG09864, Df(2L)Exel7080 and *F Df(2L)Exel7079. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182717 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC100. *F Date: Thu, 10 Mar 2005 11:37:45 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC100 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC100 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC100 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f00023 and P{XP}d04701. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f00023/ P{XP}d04701 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC100 from the segment *F of PBac{WH}f00023 to the left of its FRT site and the segment of *F P{XP}d04701 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f00023 to be at Release 3 genomic *F coordinate 21697703 on chromosome arm 2L and the insertion site of *F P{XP}d04701 to be at Release 3 genomic coordinate 21732645 on arm 2L. The *F Gene Disruption project determined the insertion site of PBac{WH}f00023 to *F be at Release 3 genomic coordinate 21697945 on arm 2L. The cytological *F breakpoints of Df(2L)BSC100 predicted from these coordinates are *F 40B1;40B3. Df(2L)BSC100 failed to complement Df(2L)Exel6049. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182718 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC101. *F Date: Thu, 10 Mar 2005 11:37:49 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC101 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC101 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC101 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f06575 and P{XP}d10944. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f06575/ P{XP}d10944 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC101 from the segment *F of PBac{WH}f06575 to the left of its FRT site and the segment of *F P{XP}d10944 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f06575 to be at Release 3 genomic *F coordinate 21599868 on chromosome arm 2L and the insertion site of *F P{XP}d10944 to be at Release 3 genomic coordinate 21662045 on arm 2L. The *F Gene Disruption project determined the insertion site of P{XP}d10944 to be *F at Release 3 genomic coordinate 21662045 on arm 2L. The cytological *F breakpoints of Df(2L)BSC101 predicted from these coordinates are *F 40A2;40A5. Df(2L)BSC101 failed to complement Df(2L)Exel6049. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182719 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC102. *F Date: Thu, 10 Mar 2005 11:37:53 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC102 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC102 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC102 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f02710 and P{XP}d10944. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f02710/ P{XP}d10944 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC102 from the segment *F of PBac{WH}f02710 to the left of its FRT site and the segment of *F P{XP}d10944 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f02710 to be at Release 3 genomic *F coordinate 21590694 on chromosome arm 2L and the insertion site of *F P{XP}d10944 to be at Release 3 genomic coordinate 21662045 on arm 2L. The *F Gene Disruption project determined the insertion site of PBac{WH}f02710 to *F be at Release 3 genomic coordinate 21590693 on arm 2L and the insertion *F site of P{XP}d10944 to be at Release 3 genomic coordinate 21662045 on arm *F 2L. The cytological breakpoints of Df(2L)BSC102 predicted from these *F coordinates are 40A2;40A5. Df(2L)BSC102 failed to complement Df(2L)Exel6049. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182720 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC103. *F Date: Thu, 10 Mar 2005 11:37:56 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC103 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC103 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC103 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f05122 and P{XP}d00641. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f05122/ P{XP}d00641 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC103 from the segment *F of PBac{WH}f05122 to the left of its FRT site and the segment of *F P{XP}d00641 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f05122 to be at Release 3 genomic *F coordinate 21491266 on chromosome arm 2L and the insertion site of *F P{XP}d00641 to be at Release 3 genomic coordinate 21509695 on arm 2L. The *F Gene Disruption project determined the insertion site of P{XP}d00641 to be *F at Release 3 genomic coordinate 21509890 on arm 2L. The cytological *F breakpoints of Df(2L)BSC103 predicted from these coordinates are *F 39E6;39E7. Df(2L)BSC103 fails to complement P{PZ}cul-202074. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182721 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC106. *F Date: Thu, 10 Mar 2005 11:38:06 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC106 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC106 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC106 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d03548 and PBac{WH}f03258. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F P{XP}d03548/PBac{WH}f03258 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC106 from the segment *F of P{XP}d03548 to the left of its FRT site and the segment of *F PBac{WH}f03258 to the right of its FRT site. Its presence was verified *F using the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of P{XP}d03548 to be at Release 3 genomic *F coordinate 291646 on chromosome arm 2L and the insertion site of *F PBac{WH}f03258 to be at Release 3 genomic coordinate 419212 on arm 2L. The *F Gene Disruption project determined the insertion site of P{XP}d03548 to be *F at Release 3 genomic coordinate 291729 on arm 2L and the insertion site of *F PBac{WH}f03258 to be at Release 3 genomic coordinate 419612 on arm 2L. The *F cytological breakpoints of Df(2L)BSC106 predicted from these coordinates *F are 21B8;21C4. It failed to complement P{lacW}U2af38k14504, al1 and *F P{lacW}RpI135k16513. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182722 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC107. *F Date: Thu, 10 Mar 2005 11:38:09 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC107 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC107 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC107 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d00080 and PBac{WH}Ipk2f05607. The deletion was isolated *F as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 *F w1118; P{XP}d00080/ PBac{WH}Ipk2f05607 males crossed to w1118; *F P{w+mC=hs-hid}2, wgSp-1/CyO females. These males were heat shocked as *F larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 *F (FBrf0175003). This cross and crosses in preceding and succeeding *F generations maintained the original genetic background of the Exelixis *F insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; *F FBrf0175002). The recombination event generated the genetic element *F P+PBac{XP5.WH5}BSC107 from the segment of P{XP}d00080 to the left of its *F FRT site and the segment of PBac{WH}Ipk2f05607 to the right of its FRT *F site. Its presence was verified using the PCR methods and primers *F described in Parks et al. Exelixis, Inc. determined the insertion site of *F P{XP}d00080 to be at Release 3 genomic coordinate 432761 on chromosome arm *F 2L and the insertion site of PBac{WH}Ipk2f05607 to be at Release 3 *F genomic coordinate 576298 on arm 2L. The Gene Disruption project *F determined the insertion site of PBac{WH}Ipk2f05607 to be at Release 3 *F genomic coordinate 576406 on arm 2L. The cytological breakpoints of *F Df(2L)BSC107 predicted from these coordinates are 21C5;21D1. It failed to *F complement ex1, ush2 and P{lacW}Nlek13714 for lethality. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182723 *a Deal *b J. *c K. *d Cook *t 2005.3.10 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC109. *F Date: Thu, 10 Mar 2005 11:38:12 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC109 *F Cc: kaufman@bio.indiana.edu, jedeal@bio.indiana.edu, medeal@bio.indiana.edu, *F Rachel Andrade *F Isolation and characterization of Df(2L)BSC109 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC109 was isolated as a FLP recombinase-induced recombination event *F involving PBac{RB}e00855 and P{XP}d09701. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{RB}e00855/ P{XP}d09701 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.RB3}BSC109 from the segment *F of PBac{RB}e00855 to the left of its FRT site and the segment of *F P{XP}d09701 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al with the substitution *F of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' *F minus primer in the Hybrid PCR protocol in the Supplementary *F Methods. Exelixis, Inc. determined the insertion site of PBac{RB}e00855 to *F be at Release 3 genomic coordinate 5066024 on chromosome arm 2L and the *F insertion site of P{XP}d09701 to be at Release 3 genomic coordinate 5138071 *F on arm 2L. The Gene Disruption project determined the insertion site of *F PBac{RB}e00855 to be at Release 3 genomic coordinate 5066024 on arm *F 2L. The cytological breakpoints of Df(2L)BSC109 predicted from these *F coordinates are 25C4;25C8. It failed to complement PBac{RB}e02157. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182730 *a Devenport *b D. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50509. *F Date: Wed, 2 Mar 2005 13:22:33 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50509 *F To: dd245@cam.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Danelle, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The FERM protein, fermitin, is part of the core integrin adhesion complex that *F is essential for integrin function during morphogenesis. *F You mention gene symbols that are new to FlyBase, Fit1 and Fit2. Do *F you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F With best wishes, *F Rachel. *F From: Danelle Devenport *F Subject: Re: Helping FlyBase: ADRC-50509 *F Date: Wed, 2 Mar 2005 14:26:01 \+0000 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F We have named CG14991 'fit1' and CG7729 'fit2', which are short for *F 'fermitin1' and 'fermitin2', respectively. *F As far as we know, no other synonyms have been used for CG14991 or *F CG7729. We chose the name 'fermitin' because it is descriptive about *F its domain composition (a single FERM domain), ends with 'in' like *F other proteins it cooperates with (integrin, talin, paxillin), and its *F catchy too! *F Is it a suitable time for me to contact fly base about making these *F officical synonyms? Or does one usually wait until the names have been *F published in a scientific journal? *F Thanks very much, *F Danelle # *U FBrf0182749 *a Dura *b J.M. *t 2005.12.4 *T personal communication to FlyBase *u *F Date: Wed, 09 Mar 2005 15:03:24 \-0500 *F To: gm119@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Dura insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Jean-Maurice Dura, Institut de Genetique Humaine, CNRS *F Montpellier (12/04). *F The following are homozygous viable, second chromosome insertions: *F P{UAS-mor.LZ}2 *F P{UAS-Alh.L-Delta1-53}2 *F P{UAS-Alh.L-DeltaLZ}2 *F P{UAS-LZ-AF10}2 *F P{UAS-MLL-Alh}2 *F P{UAS-Alh.L-PHD}2 *F The following are second chromosome insertions: *F P{Mlp84B+t10}2 *F P{UAS-MLL-AF10}2 *F The following are homozygous viable, third chromosome insertions: *F P{UAS-Alh.L-DeltaPHD}3 *F P{UAS-Alh.S}3 *F P{da-GAL4.w-}3 *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182755 *a Ekstrom *b K. *c D. *d Hultmark *t 2005.3 *T personal communication to FlyBase *u P{UAS-He.Z} insertions. *F Date: Wed, 16 Mar 2005 14:40:19 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-He.Z} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Karin Ekstrom and Dan Hultmark of Umea University (3/05). *F P{UAS-He.Z}15b is a homozygous viable and fertile, second chromosome insertion. *F P{UAS-He.Z}7c is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0182785 *a Fish *b M. *c A. *d Groth *t 2005.2.4 *T personal communication to FlyBase *u Progenitor of P{CARY.attP}. *F Date: Fri, 4 Feb 2005 19:36:21 \-0500 (EST) *F From: Kathy Matthews *F Subject: progenitor of P{CARY.attP} *F To: flybase-updates@morgan.harvard.edu *F P{CARY.attP} is almost certainly derived from P{Car20y} and therefore *F carries y+t7.7. In trying to determine the genotype details for the *F Bloomington stock records, I asked Matt Fish if this was the case and *F he provided this quote from Amy Groth: *F 'The plasmid pCaryP was created by removing attP from pTAattP (GROTH *F et al. 2000) by EcoRI digestion, filling in with T4 DNA polymerase and *F cloning it into the SmaI site of pCary, a plasmid containing the yellow *F body color gene (gift of the Bruce Baker lab).' *F P{Car20y} is the obvious suspect for pCary, and it is the only transgene *F construct in FlyBase that is consistent with 'Car' in the plasmid symbol *F and the characterisitcs of pCary. # *U FBrf0182821 *a Garrity *b P. *c A. *d Patapoutian *e V. *f Viswanath *g T. *h Jegla *t 2005.2.23 *T personal communication to FlyBase *u dANKTM1 to dTRPA1. *F Date: Thu, 24 Feb 2005 14:30:35 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: dANKTM1 to dTRPA1 *F To: rd120@gen.cam.ac.uk, pgarrity@MIT.EDU *F Cc: vviswana@gnf.org, ardem@scripps.edu, tjegla@gnf.org *F Dear all, *F Thanks for quick turnaround on this. *F Few points: *F We do not prefix symbols in FlyBase with d for Drosophila (see note at *F http://fbserver.gen.cam.ac.uk:7081/docs/nomenclature/lk/nomenclature.html#1.2. *F for explanation). *F The use of all caps to denote genes is discouraged, as this causes a *F lack of distinction between gene and gene product. See *F http://fbserver.gen.cam.ac.uk:7081/docs/nomenclature/lk/nomenclature.html#11.1. *F '11.1. Proteins.' *F for explanation of this. *F In terms of initial letter, this new name seems to be based on homology, *F so the initial letter will be upper case *F See *F http://fbserver.gen.cam.ac.uk:7081/docs/nomenclature/lk/nomenclature.html *F '1.1.1. Case of initial letter.' *F I presume you are already aware of the pre-existing gene trp:transient *F receptor potential (FBgn0003861) \- there will be a danger of your gene *F being confused with this. trp is a very well established gene symbol and *F there is no possibility of changing it. *F Having said all that, the new symbol:name will be *F TrpA1:Transient receptor potential A1 *F It will be a while (months, unfortunately) before the next update of *F genes information on the FlyBase public servers, when this name change *F will become evident, but I'll start the update process today. *F Best regards, *F Rachel. *F Cc: Veena Viswanath , ardem Patapoutian , *F Tim Jegla *F From: Paul Garrity *F Subject: Re: dANKTM1 to dTRPA1 *F Date: Wed, 23 Feb 2005 16:05:24 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel \- We are all in agreement that the name should be changed to *F dTRPA1. *F Please see attached notes from Tim and from Veena. *F \- thanks \- Paul *F Begin forwarded message: *F > From: 'Tim Jegla' *F > Date: February 23, 2005 11:53:15 AM EST *F > To: *F > *F > Hi Paul, *F > *F > Congratulations on the paper!  I think you should change the name of *F > Drosophila ANKTM1 should be Changed to TRPA1 to reflect its orthology *F > to mammalian TRPA1 genes.  There will be much less confusion in the *F > field in the future if this name change is made. *F > *F > Tim Jegla, Ph.D. *F > Neurobiology Group Leader *F > Genomics Institute of the Novartis Research Foundation *F > 10675 John Jay Hopkins Dr. *F > San Diego, CA 92121 *F > Tel:  (858)-812-1540 *F > Fax: (858)-812-1920 *F > *F > tjegla@gnf.org *F > *F Begin forwarded message: *F > From: 'Veena Viswanath' *F > Date: February 23, 2005 1:31:36 PM EST *F > To: *F > *F > Hello Paul, *F > I agree that the name of dANKTM1 should be changed to dTRPA1 in keeping *F > with the nomenclature, *F > Thanks, *F > Veena Viswanath *F >> From: Paul Garrity *F >> Subject: Re: dANKTM1 *F >> Date: Wed, 23 Feb 2005 10:05:29 \-0500 *F >> To: 'Rachel Drysdale (Genetics)' *F >> *F >> Hi Rachel \- No problem. Ardem patapoutian (the corresponding author *F >> of *F >> the viswanath paper) is a co-author on our paper. I have e-mailed *F >> him. *F >> \- paul *F >> *F >> *F >> On Feb 23, 2005, at 9:40 AM, Rachel Drysdale (Genetics) wrote: *F >> *F >>> Dear Paul, *F >>> *F >>> FBgn0035934 was named Anktm1 on account of *F >>> FBrf0160224 == Viswanath, 2003.5.19, GenBank/EMBL/DDBJ: AY302598 *F >>> and *F >>> FBrf0159694 == Viswanath et al., 2003, Nature 423(6942): 822--823 *F >>> *F >>> This naming was entirely legitimate, and we do not change gene *F >>> symbols *F >>> without good reason (and we generally do not regard name changes in *F >>> other *F >>> organisms as good reason). *F >>> *F >>> When someone wants a gene symbol change we require the agreement of *F >>> those who originally named it and those working on the gene (in this *F >>> case that would minimally be Viswanath and Jegla) not only on what *F >>> the *F >>> gene symbol should be, but also that you will all use the new symbol *F >>> in *F >>> future publications. So if you want to pursue this please could you *F >>> email Viswanath and Jegla, and then get back to me, with a *F >>> coordinated *F >>> request. We document such name changes in this way so that we do not *F >>> later reverse the name change, when working from the usual precedence *F >>> criteria. *F >>> *F >>> This might not be quite the reply you were hoping for, but we have *F >>> had *F >>> to adopt this ruthlessly pragmatic way of dealing with nomenclature, *F >>> otherwise (we have learned by experience) mediating nomenclature *F >>> discussions would take up a huge proportion of our time for no real *F >>> gain of data in FlyBase. *F >>> *F >>> With best regards, *F >>> *F >>> Rachel *F >>> for FlyBase. *F >>> *F >>> *F >>> *F >>> *F >>> *F >>>> To: flybase-updates@morgan.harvard.edu *F >>>> From: Paul Garrity *F >>>> Subject: dANKTM1 *F >>>> Date: Tue, 22 Feb 2005 16:29:04 \-0500 *F >>>> *F >>>> I wanted to request that the name of the gene dANKTM1 *F >>>> (http://flybase.net/.bin/fbidq.html?FBgn0035934) be changed to *F >>>> dTRPA1 *F >>>> to reflect recent standardization of nomenclature in the TRP field. *F >>>> *F >>>> In vertebrates, ANKTM1 is now commonly referred to as TRPA1 \--- for *F >>>> example, the recent Nature article: ' TRPA1 is a candidate for the *F >>>> mechanosensitive transduction channel of vertebrate hair cells.' *F >>>> D.P. *F >>>> Corey et al. (2004) Nature 432:723-730. *F >> *F >>>> We recently published loss-of-function analysis of the fly TRPA1 *F >>>> ortholog in G&D \--- 'The Drosophila ortholog of vertebrate TRPA1 *F >>>> regulates thermotaxis.' M. Rosenzweig, K.M. Brennan, T.D. Tayler, *F >>>> P.O. *F >>>> Phelps, A. Patapoutian, and P.A. Garrity (2005). Genes & Development *F >>>> 19:419-424. *F >>>> *F >>>> \- thank you \- *F >>>> Paul Garrity *F >>>> *F >>>> *F >>>> Paul Garrity *F >>>> MIT Dept. of Biology *F >>>> 31 Ames Street *F >>>> Building 68 Room 230B *F >>>> Cambridge, MA 02139-4307 *F >>>> *F >>>> Office: 617-258-8144 *F >>>> Lab: 617-253-0808 *F >>>> FAX: 617-253-3128 *F >>>> http://web.mit.edu/biology/garrity # *U FBrf0182831 *a Ghabrial *b A. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50662. *F Date: Wed, 2 Mar 2005 13:28:51 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50662 *F To: ghabrial@cmgm.stanford.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Amin, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Control of tracheal terminal cell size and branching. *F You mention gene symbols that are new to FlyBase, whacked, winded and *F miracle-gro. Do you know which of the Genome Project CG annotations *F your genes correspond to? All the CGs have corresponding gene records *F in FlyBase already and we don't like to make duplicate records for what *F is actually the same gene unless we can't avoid it. The CG symbols *F become synonyms when an annotation is named with a more descriptive or *F functional name. *F Also, if you have settled on short symbols for your genes now would be *F a good time to let me know what they are, as then I can enter them into the *F database from the outset. *F With best wishes, *F Rachel. *F Date: Wed, 2 Mar 2005 10:28:42 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: Amin Ghabrial *F Subject: Re: Helping FlyBase: ADRC-50662 *F Dear Rachel, *F Thank you for contacting me. We now know that miracle-gro is *F allelic to warts and that winded is allelic to cdsA. whacked disrupts *F CG5344. The exelixis piggyBac 'allele,' e01976 is misannotated as *F it inserts some 2kb 5' of whacked (and probably disrupts CG5342). We *F have settled on wkd as the short symbol for whacked. Sorry about the *F extra names (miracle-gro and winded) but as soon as mutations fall *F into a complementation group it becomes much easier to talk about *F them by a single descriptive name then by all the allele numbers. *F Cheers, *F Amin *F Date: Fri, 4 Mar 2005 11:50:11 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: Amin Ghabrial *F Subject: Re: Helping FlyBase: ADRC-50662 *F Dear Rachel, *F In my last email I had said that the e01976 piggyBac is probably *F not an allele off CG5344/whacked but may be an allele of CG5342. I *F mistakenly said that the piggyBac insertion site was 2 kb 5' of *F whacked. It is actually more like 200 bp. However, by *F complementation e01976 is not allelic to whacked, and the insertion *F site of e01976 seems to be in the CG5342 transcription unit (based on *F the sequence of AT05577.3prime). Thanks for your help. *F Cheers, *F Amin *F \-- # *U FBrf0182836 *a Gilfillan *b J.C. *t 2005.3.2 *T personal communication to FlyBase *u *F Date: Wed, 2 Mar 2005 13:47:40 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50219 *F To: j.c.gilfillan@open.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Joanne, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Molecular genetic characterisation of Glutathione Synthetase in Drosophila *F melanogaster. *F You mention a gene symbol that is new to FlyBase, GS. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Subject: RE: Helping FlyBase: ADRC-50219 *F Date: Wed, 2 Mar 2005 17:11:18 \-0000 *F From: 'J.C.Gilfillan' *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel *F The gene I'm working on is CG6835, glutathione synthetase *F Please write back if you need more information *F Thanks, *F Joanne # *U FBrf0182844 *a Goberdhan *b D. *t 2005.3.11 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50170. *F Date: Wed, 2 Mar 2005 13:45:19 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50170 *F To: deborah.goberdhan@anat.ox.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Deborah, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A specific amino acid transporter regulates TOR-dependent growth via a *F novel nutrient sensing mechanism. *F You mention a gene symbol that is new to FlyBase, pathetic. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if you have settled on a short symbol for your gene now would be *F a good time to let me know what it is, as then I can enter it in the *F database from the outset. *F With best wishes, *F Rachel. *F From: 'Deborah Goberdhan' *F To: ''Rachel Drysdale \(Genetics\)'' *F Subject: RE: Helping FlyBase: ADRC-50170 *F Date: Fri, 11 Mar 2005 11:56:29 \-0000 *F Dear Rachel *F Apologies for delay in replying. pathetic corresponds to CG3424 and the *F abbreviation we have settled on is path. *F Thank you for dealing with this. *F With best wishes *F Deborah # *U FBrf0182867 *a Green *b M. *t 2005.2.15 *T personal communication to FlyBase *u Sxl allele on Binsc, oc1 ptg1. *F Date: Tue, 15 Feb 2005 15:30:37 \-0500 (EST) *F From: Kathy Matthews *F Subject: Sxl allele on Binsc, oc1 ptg1 *F To: flybase-updates@morgan.harvard.edu *F Personal communication from: Mel Green, U.C. Davis *F To: Bloomington Drosophila Stock Center *F Subject: Sxl allele on Binsc, oc1 ptg1 *F Dated: 15 Feb 2005 *F The Binsc variant marked with oc1 and ptg1, Binscop (FBba0000392), *F is viable when hemizygous and lethal when homozygous. Mel Green did a *F complementation test with this chromosome and a Sxl allele (he did not *F specify the allele) and found failure to complement, indicating that *F Binscop carries a Sxl allele. This is almost certainly Sxlf1, *F introduced via the In(1)dl-49 component of Binsc. # *U FBrf0182873 *a Grillo-Hill *b B.K. *t 2005.3.28 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50709. *F Date: Mon, 28 Mar 2005 09:34:54 \-0600 (CST) *F From: 'Bree K. Grillo-Hill' *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50709 *F Hi Rachel, *F .. *F The gene I have been working with, referred *F to as 'icon' and 'inappropriate contacts' is allelic to 'hibris' also *F known as CG7449. Please let me know if you need any more information. *F Thank you for your time and efforts, *F Bree Grillo-Hill *F > We are currently curating the abstracts for the upcoming 46th *F > (San Diego) Annual Drosophila Research Conference, for FlyBase. *F > I am writing in connection with your abstract: *F > Genetic Regulation of Cone Cell Contacts during Pupal Eye Development. # *U FBrf0182890 *a Hafer *b N. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50201. *F Date: Wed, 2 Mar 2005 13:46:32 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50201 *F To: nhafer@molbio.princeton.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Nathaniel, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Analysis of an orb related gene, orb2, in the Drosophila central nervous system. *F You mention a gene symbol that is new to FlyBase, orb2. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F From: 'Hafer, Nathaniel' *F To: ''Rachel Drysdale (Genetics) '' *F Subject: RE: Helping FlyBase: ADRC-50201 *F Date: Wed, 2 Mar 2005 08:58:56 \-0500 *F Hi Rachel, *F The CG annotation for orb2 is CG5735. Please let me know if you have any *F other questions about this gene! *F Nate # *U FBrf0182897 *a Hanson *b K. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50471. *F Date: Wed, 2 Mar 2005 13:21:12 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50471 *F To: kkhanson@mrc-lmb.cam.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Kirsten, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Loss of Borealin function causes cell autonomous mitotic defects and has *F non-autonomous effects on tissue development. *F You mention a gene symbol that is new to FlyBase, Bor. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Wed, 02 Mar 2005 15:59:40 \-0000 *F From: Kirsten Hanson *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50471 *F Hello, *F The gene we refer to as dBor is CG4454. If you need more information, just *F let me know. *F best, *F Kirsten # *U FBrf0182964 *a Ishikawa *b H. *t 2005.3.7 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50099. *F Date: Wed, 2 Mar 2005 13:42:39 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50099 *F To: hoishika@rs.noda.tus.ac.jp *F Cc: rd120@gen.cam.ac.uk *F Dear Hiroyuki, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A Drosophila model for human congenital disorder of glycosylations IIc. *F You mention a gene symbol that is new to FlyBase, Gfr. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F Might it be CG9620? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F With best wishes, *F Rachel. *F Date: Mon, 7 Mar 2005 23:33:40 \+0900 *F To: 'Rachel Drysdale (Genetics)' *F From: 'Hiroyuki O. Ishikawa' *F Subject: Re: Helping FlyBase: ADRC-50099 *F Dear Rachel, *F First, I apologize that we did not note the CG number of Golgi GDP-fucose *F transporter (gfr) gene in the abstract. *F As you pointed out, gfr is CG9620. *F We have confirmed Gfr localized to the Golgi, and had an activityto *F transport GDP-fucose into the Golgi. *F So we believe that gfr is a reasonable name for CG9620. *F Best regards, *F Hiro. # *U FBrf0182973 *a Janody *b F. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50357. *F Date: Wed, 2 Mar 2005 13:51:34 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50357 *F To: janody@ibdm.univ-mrs.fr *F Cc: rd120@gen.cam.ac.uk *F Dear Florence, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Miro, Twinstar and Capulet all restrict actin filament polymerization *F but have distinct developmental functions. *F You mention a gene symbol that is new to FlyBase, miro. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Wed, 2 Mar 2005 15:57:02 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Florence Janody *F Subject: Re: Helping FlyBase: ADRC-50357 *F Dear Rachel, *F Sorry for the confusing name I gave to this gene. I just saw the *F abstract for the upcoming 46th Annual Drosophila Research Conference *F about dMiro which is not the same gene. *F The miro gene, I characterized , encodes for the capping protein of *F alpha type (CG10540). *F I'll change this name very soon. *F Best wishes. *F Florence # *U FBrf0182976 *a Javier *b A. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50921. *F Date: Wed, 2 Mar 2005 13:39:18 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50921 *F To: ajavier@uci.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Anna, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A conserved genetic pathway regulates nuclear positioning. *F You mention a gene symbol that is new to FlyBase, nudE. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Tue, 8 Mar 2005 11:55:26 \-0800 (GMT-08:00) *F From: Anna Javier *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50921 *F Just in case I didn't get through last time.... NudE in Drosophila *F corresponds to CG8104. *F Anna Javier # *U FBrf0182997 *a Joyce *b E. *t 2005.3.3 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50417. *F Date: Wed, 2 Mar 2005 13:53:53 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50417 *F To: ejoyce20@yahoo.com *F Cc: rd120@gen.cam.ac.uk *F Dear Eric, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A meiotic mutant with a delay in the recombination pathway has reduced crossing *F over. *F You mention a gene symbol that is new to FlyBase, g7. Do you know *F yet which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if g7 has picked up a more descriptive name/symbol since you *F submitted the abstract then this would be a good time to tell me so I *F can get it into the database. *F With best wishes, *F Rachel. *F Date: Thu, 3 Mar 2005 09:13:07 \-0800 (PST) *F From: Eric Joyce *F Subject: Re: Helping FlyBase: ADRC-50417 *F To: 'Rachel Drysdale \(Genetics\)' *F Dear Rachel, *F g7 is an allele of the gene CG15329. We are currently *F undecided for a better name that corresponds to it's *F phenotype. If we come up with a name soon I will be *F sure to let you know as soon as possible. Please let *F me know if you need anything else. Thank you. *F Eric # *U FBrf0183003 *a Ratnakumar *b K. *c C. *d Desplan *t 2004.12 *T personal communication to FlyBase *u P{rh1-GAL4} and P{Rh4-GAL4} insertions. *F Date: Fri, 04 Mar 2005 15:03:27 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{rh1-GAL4} and P{Rh4-GAL4} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York University *F (12/04). *F P{rh1-GAL4}1 and P{Rh4-GAL4}1 are homozygous and hemizygous viable and *F fertile, X chromosome insertions. *F P{Rh4-GAL4}2 and P{rh1-GAL4}2 are second chromosome insertions. *F P{rh1-GAL4}3 and P{Rh4-GAL4}3 are homozygous viable and fertile, third *F chromosome insertions. *F These insertions originated in the lab of Jessica Triesman. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0183007 *a Kanagawa *b S. *c A.W. *d Moore *t 2004.11.18 *T personal communication to FlyBase *u *F Date: Thu, 18 Nov 2004 15:51:00 \+0900 *F To: flybase-updates@morgan.harvard.edu *F From: Adrian Moore *F Subject: Unpublished Data Submission to FlyBase *F Cc: kanagawa@postman.riken.go.jp *F Dear Sir, *F We would like to submit the data we have obtained for A1-2-29 *F enhancer-trap line to FlyBase, since we are not planning to publish *F the data. *F We rescued both 5' and 3' flanking genomic sequence of the pLacW *F insertion by plasmid rescue method and the details are shown below. *F Enhancer-trap line: A1-2-29 *F Reference: Hartenstein and Jan 1992, FBrf0057603 *F Insertion was found to be upstream of the gene Rapgap1 FBgn0014015 *F 3' rescued sequence \+/- (starting at 2L: 7522842) *F CATGGGCAGAGGCAGCAAACAAC *F ATTGGATATTTTTCAGTTGCCGTTTCTCCGTTGCCGAAAGCAGACGTTTGAGCCGGTTTGAATTC *F 5' rescued sequence \+/+ (starting at 2L: 7522829) *F TGCCTCTGCCGATGCGTCTGTCG *F CTCTGCTCTGCCAACGCACAGTG *F GGCCAGACACAACAAGACTCGAT *F GTCTAAGAAAAGCGCATTTTGCG *F TACGTATGTTATCCATATGGGGA *F TTTGTAAAAATTTCTGTTATTTA *F TAACATACATTATTCTATTATATACAAAATGGTTTTTTGAGAAATTTA *F Reference for Rapgap1: *F Biological characterization of Drosophila Rapgap1, a GTPase *F activating protein for Rap1. *F Chen, F., et al. *F Proc. Natl. Acad. Sci. USA *F Vol. 94, pp. 12485-12490 1997 *F If you use this Information, please quote pers. comm. from: Kanagawa S. and *F Moore A.W. *F Regards *F Adrian Moore *F Dr Adrian Moore, *F Unit Leader, *F Molecular Neuropathology Group, *F RIKEN Brain Science Institute, *F 2-1 Hirosawa, Wako City, Saitama *F 351-0198 JAPAN *F Tel 048 467 7274 *F Fax 048 462 4796 # *U FBrf0183014 *a Karagiosis *b S. *t 2005.1.5 *T personal communication to FlyBase *u *F Date: Wed, 05 Jan 2005 14:26:25 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: UAS-Moe insertions *F The following information accompanied stocks donated the Bloomington Stock *F Center by Sue Karagiosis, Purdue University (11/04). *F P{UAS-Moe.GFP.K}2, P{UAS-Moe.T559D.MYC}2 andP{UAS-Moe.MYC.K}2 are *F homozygous viable and fertile, second chromosome insertions. *F P{UAS-Moe.IR.327-775}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0183037 *a Kim *b Y.O. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50410. *F Date: Wed, 2 Mar 2005 13:53:19 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50410 *F To: kimyon@nhlbi.nih.gov *F Cc: rd120@gen.cam.ac.uk *F Dear Yong-Ou, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Role of the sprite gene encoding a PDZ domain protein in the Drosophila heart *F development. *F You mention a gene symbol that is new to FlyBase, sprt. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F From: 'Kim, Yong-On (NIH/NHLBI)' *F To: ''Rachel Drysdale (Genetics)'' *F Subject: RE: Helping FlyBase: ADRC-50410 *F Date: Wed, 2 Mar 2005 12:18:46 \-0500 *F Dear Rachel, *F The CG number for the sprite is CG30023. *F Thanks *F Yong-Ou Kim, PhD *F NIH/NHLBI *F Bethesda, MD 20892, USA *F 301-496-3470 # *U FBrf0183040 *a Kitsou *b E. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50002. *F Date: Wed, 2 Mar 2005 13:19:51 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50002 *F To: effie@ludwig.ucl.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Effie, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The role of the PDZ domain in the function of RhoGEF2 in cell shape changes *F during Drosophila morphogenesis. *F You mention a gene symbol that is new to FlyBase, Mec2. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Wed, 2 Mar 2005 15:53:24 \+0000 *F To: 'Rachel Drysdale (Genetics)' *F From: Effie Kitsou *F Subject: Re: Helping FlyBase: ADRC-50002 *F Dear Rachel, *F The drosophila Mec2 corresponds to CG7635. *F CG7635 has a high sequence similarity with the C.elegans Mec2 and the human *F gene called stomatin. *F Best wishes, *F Effie # *U FBrf0183052 *a Korey *b C. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50168. *F Date: Wed, 2 Mar 2005 13:44:57 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50168 *F To: koreyc@cofc.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Christopher, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Studying Infant and Juvenile Onset NCL in Drosophila. *F You mention a gene symbol that is new to FlyBase, cln3. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. What *F would you like the full name for cln3 to be \- may as well enter it in *F the database from the outset. *F With best wishes, *F Rachel. *F Date: Wed, 02 Mar 2005 08:56:21 \-0500 *F Subject: Re: Helping FlyBase: ADRC-50168 *F From: Chris Korey *F To: 'Rachel Drysdale (Genetics)' *F Rachel, *F The CG annotation is CG5582. The human gene is Cln3. The homologous *F drosophila gene could be called DmCln3. Let me know if you need anything *F else. *F Chris *F \-------- *F Christopher Korey, Ph.D. *F Assistant Professor *F Department of Biology *F College of Charleston *F 66 George Street *F Charleston, SC 29424 *F 843-953-7178 *F www.cofc.edu/~koreyc # *U FBrf0183054 *a Kornberg *b T. *c H. *d Salz *e S. *f Tanda *t 2005.1.24 *T personal communication to FlyBase *u Gene renaming. *F Date: Mon, 24 Jan 2005 17:34:00 \-0800 *F To: flybase-updates@morgan.harvard.edu *F From: Tom Kornberg *F Subject: Gene renaming *F Cc: Kevin Cook , Helen Salz *F I write to request a name change for a locus. *F We have determined the enzymatic function for the gene previously *F designated 'shanti' and will in all future publications refer to this *F gene as: signal peptide protease, abbreviated spp. I was informed *F that Helen Salz had previously used the designation spp for a SNF *F protein, but she has graciously agreed to defer and to allow us to *F use spp for the Drosophila signal peptide protease gene. *F Thank you. *F \-- *F Thomas Kornberg *F Department of Biochemistry *F University of California *F San Francisco, CA 94143 *F Phone: 415-476-8821 *F Fax: 415-514-1470 *F email: tkornberg@biochem.ucsf.edu *F \------------------------------------------------------------------------- *F Date: Tue, 25 Jan 2005 11:21:19 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: Gene renaming *F To: flybase-updates@morgan.harvard.edu, tkornberg@biochem.ucsf.edu *F Cc: kcook@bio.indiana.edu, hks@po.cwru.edu *F Dear Tom, *F 'shanti' (FBgn0031260) was named in *F FBrf0134637 == Apionishev et al., 2001, Genes to Cells 6(3): 215--224 *F and although it was not a main focus of the paper the naming is *F legitimate, and the name has been used in at least one later *F publication \- *F FBrf0167608 == Greil et al., 2003, Genes Dev. 17(22): 2825--2838 *F For FlyBase to change the symbol of shanti we would need you to *F coordinate agreement with the Apionishev group (cc'd to FlyBase), with *F an assurance that they would use spp in publications in the future. *F . *F . *F Helen's gene is currently in FB as 'Spp:SNFs Protein Partner' *F (FBgn0038041). The upper case 'S' in is accordance with the community *F gene naming rules 'Genes named after a protein product or other *F molecular feature begin with an uppercase letter' *F (http://flybase.bio.indiana.edu/docs/nomenclature/lk/nomenclature.html#1.1.). *F The 'spp' choice for shanti (FBgn0031260) is not ideal. Helen's group *F has used 'spp' for Spp (FBgn0038041) in the literature and so spp will *F remain a synonym for Spp in FB even were spp to be adopted as a valid symbol *F for shanti. This means that symbol/synonym searches for 'spp' would *F return both Spp (FBgn0038041)and spp (FBgn0031260). This is not necessarily *F the end of the world (there are other instances of this in FlyBase) but *F it is something you should be aware of if you are going to proceed with *F this name change. Where we have influence over symbols (i.e. when *F people approach us for advice) we are steering people away from *F symbols that differ from existing symbols only by capitalization, and *F this is an example of that. *F Of course the 'Genes named after a protein product or other molecular *F feature begin with an uppercase letter' rule also implies that *F FBgn0031260 (your spp i.e. shanti) should have an initial upper case *F letter ('signal peptide protease' being a protein product name), but *F that would bring it into conflict with Helen's Spp (FBgn0038041).... *F You did say 'Helen ... has graciously agreed to defer' but didn't *F suggest a new symbol for FBgn0038041. *F . *F . *F These factors add up to a strong case for not choosing spp/Spp as symbols *F for FBgn0031260/FBgn0038041, in fact for not renaming shanti at all. *F However if you do want to pursue this I'd be happy to check out the relative *F merits of any other suggestions you might have, before you approach the *F Apionishev group. *F With best regards, *F Rachel. *F for FlyBase. *F \------------------------------------------------------------------------- *F To: 'Rachel Drysdale (Genetics)' *F Cc: kcook@bio.indiana.edu, flybase-updates@morgan.harvard.edu, *F hks@po.cwru.edu, tkornberg@biochem.ucsf.edu *F From: Helen Salz *F Subject: Re: Gene renaming *F Date: Tue, 25 Jan 2005 09:08:00 \-0500 *F Good morning Rachel, Tom, Kevin etc. *F The name Spp: SNF's protein partner (ie CG6525) has really only *F appeared in fly base, and meeting abstracts. We have not yet published *F our first paper on the characterization of this gene, and we are happy *F to revert to CG6525 until we know more about its mutant phenotype. *F Once we know more, we will try and come up with a clever name that is *F not in use by anyone else. *F \------------------------------------------------------------------------- *F Helen Salz, PhD *F Professor, Department of Genetics *F Case Western Reserve University *F (216) 368-2879 *F \------------------------------------------------------------------------- *F Date: Tue, 25 Jan 2005 08:22:48 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: Tom Kornberg *F Subject: Re: Gene renaming *F Cc: 'Soichi Tanda' *F Dear Rachel- *F I am not sure I follow all your arguments here, but I will try to *F clarify the issues as I understand them. *F Regarding the prior use of shanti by Apionishev et al, the work was *F carried out in the Tanda lab and Soichi Tanda is in full agreement *F with this name change. He is a collaborator in our work and a *F co-author of the publication that identifies the protein product. *F Our reasons for wanting to call this gene spp are the following: The *F signal peptide protease enzyme is now and will be known as spp in *F every other organism. There are already an extensive literature and *F numerous reviews that use spp for the mammalian signal peptide *F protease, so confusion would be inevitable if the Drosophila *F community alone uses the name differently. It behooves us to have *F Drosophila conform. *F As Helen has confirmed, she has agreed to allow us to use spp despite *F her prior use of it for the SNF protein partner her group is studying. *F Please let me know if we need to do anything further to effect this *F change. Our publication will be submitted in its final form to *F Genetics today. Unless you advise otherwise, it will designate the *F gene Spp, uppercase as you recommend. *F Thank you. *F Tom # *U FBrf0183072 *a Kunert *b N. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50381. *F Date: Wed, 2 Mar 2005 13:52:11 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50381 *F To: n.kunert@dkfz-heidelberg.de *F Cc: rd120@gen.cam.ac.uk *F Dear Natascha, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Identification and characterization of IPOD, a novel interaction *F partner of the Dnmt2 DNA methyltransferase in Drosophila. *F You mention a gene symbol that is new to FlyBase, Ipod. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Wed, 02 Mar 2005 16:12:20 \+0100 *F Subject: Re: Helping FlyBase: ADRC-50381 *F From: Natascha Kunert *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F We identified a previously uncharacterized protein, encoded by CG2961 *F (AE003449; FBgn0030187), which we named 'IPOD' (Interaction Partner Of *F Dnmt2). Please do not hesitate to contact me if there are any further *F questions. Best regards, *F Natascha Kunert *F Epigenetics *F German Cancer Research Center *F INF 580 *F D-69120 Heidelberg, Germany *F E-mail: n.kunert@dkfz-heidelberg.de # *U FBrf0183081 *a Lai *b E. *t 2005.3.15 *T personal communication to FlyBase *u *F Date: Wed, 2 Mar 2005 13:41:23 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50059 *F To: lai@fruitfly.org *F Cc: rd120@gen.cam.ac.uk *F Dear Eric, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The Ubiquitin Ligase Drosophila Mind Bomb Promotes Notch Signaling By *F Regulating The Localization And Activity Of Serrate And Delta. *F You mention a gene symbol that is new to FlyBase, mind-bomb. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F You suggest a symbol for mindbomb, mib, that is not going to be *F suitable as we already have a gene termed mib (FBgn0002744: miniature *F bristles), and we do not prefix gene symbols with D for Drosophila as a *F matter of policy. mibb is available, as is mdbb. Let me know if you *F favour either of those, or have another suggestion. *F With best wishes, *F Rachel. *F Date: Tue, 15 Mar 2005 02:39:20 \-0800 *F To: 'Rachel Drysdale (Genetics)' *F From: Eric Lai *F Subject: Re: Helping FlyBase: ADRC-50059 *F Cc: schweisg@biologie.ens.fr (Francois SCHWEISGUTH) *F hi rachel, *F yes we are aware of miniature bristle. i am afraid that it might be *F too late to rename our gene (CG5841). we had coordinated the name *F 'D-mib'with the Schweisguth group. their paper is online at PLoS and *F we have a paper in press at Development. *F regards, *F .eric # *U FBrf0183088 *a Lasko *b P. *c S. *d Levine *t 2004.12 *T personal communication to FlyBase *u P{UAS-Paip2.HA} insertions. *F Date: Fri, 04 Mar 2005 14:32:13 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Paip2.HA} insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Paul Lasko & Sylvia Levine of McGill University (12/4). *F P{UAS-Paip2.HA}I is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-Paip2.HA}II is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0183089 *a Lasko *b P. *c S. *d Levine *t 2004.12 *T personal communication to FlyBase *u P{eIF-4ESer251Asp.8.9}2 and P{eIF-4ESer251Ala.8.9}2. *F Date: Fri, 04 Mar 2005 14:17:50 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{eIF-4ESer251Asp.8.9}2 and P{eIF-4ESer251Ala.8.9}2 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Paul Lasko & Sylvia Levine of McGill University (12/4). *F P{eIF-4ESer251Asp.8.9}2 and P{eIF-4ESer251Ala.8.9}2 are second *F chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0183093 *a Lawniczak *b M. *t 2005.1.19 *T personal communication to FlyBase *u *F Date: Wed, 19 Jan 2005 11:00:31 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB-NG Help Mail: affy probes *F comments: Hi Flybase Help (&/or Sima), *F . *F . *F Also, CG15156 is probably a real gene. It appeared upregulated in two *F separate microarray analyses looking at mating influenced genes in females. *F thanks, *F mara *F realname: Mara Lawniczak *F reply-to: marakat@ucl.ac.uk *F source: FB-NG Help Mail: *F usersubject: affy probes # *U FBrf0183103 *a Leiserson *b W. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50196. *F Date: Wed, 2 Mar 2005 13:46:07 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50196 *F To: william.leiserson@yale.edu *F Cc: rd120@gen.cam.ac.uk *F Dear William, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A role for the Fray kinase in nerve glia to regulate ion homeostasis. *F You mention a gene symbol that is new to FlyBase, NCC69. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if you have let me know the full name for NCC69 I can enter it into *F the database from the outset. *F With best wishes, *F Rachel. *F Date: Wed, 2 Mar 2005 09:56:53 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F From: William Leiserson *F Subject: Re: Helping FlyBase: ADRC-50196 *F Hi Rachel, *F NCC69 corresponds to CG4357. I have tentatively named it that because *F it is a homolog of the mammalian NCC (sodium chloride cotransporter) *F and NKCC (sodium potassium chloride cotransporter). I don't yet know *F which ions pass through NCC69. The '69' refers to its location in the *F Drosophila genome (to distinguish it from NCC83). Since I submitted *F the abstract, it has come to my attention that some people object to *F the 'cotransporter' nomenclature, and prefer to use the term 'solute *F carrier.' This is to distinguish this family of molecules from the *F ABC transporters. Flybase uses the term 'symporter' for CG4357. *F However, the vast majority of the 'functional' literature refers to *F this class of molecules as cotransporters. *F That's the story behind NCC69. I don't know if Flybase wants to *F rename CG4357 at this point, or if it would be better to wait until *F we more information on its function and/or decide whether flies will *F follow the 'solute carrier' nomenclature of the genome jocks. *F Hope this is helpful. Please let me know what you decide. *F Best, *F Billy # *U FBrf0183109 *a Levine *b S. *c P. *d Lasko *t 2004.12 *T personal communication to FlyBase *u Lasko insertions. *F Date: Fri, 04 Feb 2005 14:58:33 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Lasko insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Sylvia Levine & Paul Lasko, McGill University (12/04). *F P{UAS-Thor.wt}2 and P{UAS-eIF-4E.R}2 are homozygous viable and fertile, second *F chromosome insertions. *F P{UAS-pAbp.S}3, P{UAS-Thor.LL}s (FBti0027717) and P{UAS-Thor.wt}3 are *F homozygous viable and fertile, third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0183137 *a Ly *b C. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50399. *F From: Rachel Drysdale (Genetics) *F To: cindy.ly@bcm.tmc.edu *F Cc: rd120@gen.cam.ac.uk *F Sent: 3/2/2005 7:52 AM *F Subject: Helping FlyBase: ADRC-50399 *F Dear Cindy, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Fratboy, a Dynamin related protein, affects mitochondrial morphology and *F transport and is required for mobilization of the reserve pool at the *F synapse. *F You mention a gene symbol that is new to FlyBase, fratboy. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if you have settled on a short symbol for your gene now would be *F a good time to let me know what it is, as then I can enter it in the *F database from the outset. *F With best wishes, *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F From: Cindy V Ly *F To: ''Rachel Drysdale (Genetics) '' *F Cc: Hugo Bellen *F Subject: RE: Helping FlyBase: ADRC-50399 *F Date: Wed, 2 Mar 2005 12:57:05 \-0600 *F Dear Rachel, *F We isolated 2 alleles of the fratboy (frat) complementation group *F (Verstreken et. al., 2005) in a forward genetic screen for defects in *F neurotransmitter release (Koh et. al., 2004; Verstreken et. al., 2005). *F Both frat1 and frat2 were mapped to the 22F-23A interval by recombination *F (Zhai et. al., 2004) and fail to complement Df(2L)C144, Df(2L)dppd14, and *F Df(2L)Exel6008 (Parks et. al., 2004). Additionally, the frat alleles fail *F to complement l(2)22FdT26, l(2)22FcH7 and the P element insertion KG03815 *F in CG3210 (Bellen et. al., 2004; Littleton and Bellen, 1994). CG3210 is *F also known in Flybase as dynamin-2 (Lloyd et. al., 2000), noodle or drp1 *F (van der Bliek et. al., 1999). To further confirm that CG3210 corresponds *F to frat, we sequenced the alleles and identified mutations in frat1, frat2 *F and l(2)22FcH7. Finally, we performed rescue experiments using a genomic *F rescue construct that spans the CG3210 gene. This construct rescues the *F lethality associated with homozygous frat2 and trans-heterozygous *F frat1/frat2 flies. Hence, frat corresponds to CG3210 or drp1 and we *F therefore renamed frat1 as drp11; frat2 as drp12; l(2)22FdT26 as *F drp1T26; *F l(2)22FcH7 as drp1H7 and KG03815 as drp1KG. *F Hope this helps, *F Cindy Ly *F Bellen Lab *F Department of Neuroscience *F Baylor College of Medicine # *U FBrf0183147 *a Macleod *b G. *t 2005.3.7 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50738. *F Date: Wed, 2 Mar 2005 13:31:55 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50738 *F To: gmacleod@neurobio.arizona.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Gregory, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Mutations in the Drosophila GTPase dMiro disrupt microtubules, *F mitochondrial transport, synaptic structure and function. *F You mention a gene symbol that is new to FlyBase, miro. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Mon, 7 Mar 2005 13:56:30 \-0700 *F From: gmacleod@manduca.neurobio.arizona.edu *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50738 *F Hello Rachel, *F The gene (dMiro) we are looking at is CG5410, *F the fly ortholog of human Miro (Fransson et al., 2003). *F Fransson, A., Ruusala, A., and Aspenstrom, P. (2003). *F Atypical Rho GTPases have roles in mitochondrial homeostasis *F and apoptosis. J Biol Chem 278, 6495-6502. *F Sincerely *F Greg *F Tel: 520 360 4143 # *U FBrf0183166 *a Marygold *b S. *t 2005.1.19 *T personal communication to FlyBase *u Genetics of the 36F region. *F File deposited: Marygold.2005.1.19.doc ; File date: 2005.1.19 ; File size: *F 50688 ; File format: doc # *U FBrf0183176 *a Matthews *b K. *t 2004.12.29 *T personal communication to FlyBase *u *F Date: Wed, 29 Dec 2004 18:32:23 \-0500 (EST) *F From: Kathy Matthews *F Subject: Dp(2;1)C239 *F To: flybase-updates@morgan.harvard.edu *F Dp(2;1)C239 is duplicated for hk, Ddc and pr. # *U FBrf0183177 *a Matthews *b K. *t 2005.3.20 *T personal communication to FlyBase *u Ptp10DBG02428. *F Date: Sun, 20 Mar 2005 17:25:40 \-0500 (EST) *F From: Kathy Matthews *F Subject: Ptp10DBG02428 *F To: flybase-updates@morgan.harvard.edu *F Cc: matthewk@fly.bio.indiana.edu *F A high frequency of Ptp10DBG02428 homozygotes have duplicated anterior *F scutellar bristles. # *U FBrf0183188 *a McBrayer *b Z. *t 2005.3.3 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50422. *F Date: Wed, 2 Mar 2005 13:54:15 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50422 *F To: painthorse2718@yahoo.com *F Cc: rd120@gen.cam.ac.uk *F Dear Zofeyah, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of a Drosophila prothoracicotropic hormone (PTTH) homologue. *F You mention the Ptth gene. Do you know yet which of the Genome Project *F CG annotations your gene corresponds to? All the CGs have *F corresponding gene records in FlyBase already and we don't like to make *F duplicate records for what is actually the same gene unless we can't *F avoid it. The CG symbols become synonyms when an annotation is named *F with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Thu, 3 Mar 2005 07:49:05 \-0800 (PST) *F From: Zofeyah McBrayer *F Subject: Re: Helping FlyBase: ADRC-50422 *F To: 'Rachel Drysdale \(Genetics\)' *F Cc: Zofeyah McBrayer *F Hello Rachel Drysdale, *F I am writing in response to your question regarding the CG annotation of our *F putative PTTH homologue for the following abstract title: Characterization of *F a Drosophila Prothoracicotropic Hormone (PTTH) homologue. *F The CG annotation for our PTTH is CG13687. *F Please feel free to contact me if you have anymore questions. *F Zofeyah McBrayer *F painthorse2718@yahoo.com # *U FBrf0183223 *a Misra *b S. *c J. *d Rehm *t 1999.3.12 *T personal communication to FlyBase *u *F On Fri, 12 Mar 1999, Sima Misra wrote: *F >>From j.roote@gen.cam.ac.uk Mon Mar 08 15:04:40 1999 *F >Envelope-to: m.ashburner@gen.cam.ac.uk *F >Did we ever get an answer to this ? *F This is what I had in my note in the genes file. *F >SM_notes: k14423 inserted at nt 6677 of DS09217, cDNA starts at nt 6936 *F >(259bp away), cDNA for BG:DS09217.1 starts at nt 5693 (984bp away) *F However, this was based on the sequence for k14423 in BFD, which is *F incorrect. This was in fact sequenced by Jay Rehm, and the 444bp sequence *F I have from him is: *F l(2)k14423271, 444 bases, 1779 checksum. *F GCGCTGCTTTTTGCCTGATAGAAGCTGCCAATATATTATTACTATATATA *F TATATACATGTTATTAGTCTTCGAATTAAATTCGAATATACCAATCATAT *F TTCCATAATAAAGTACAACATAATTTCTAACTATTATATTTTTTTTTCTT *F TTTTTGAACTAATTCAAAAATAGCTTAATTTCCCCTCAAAATAGTCAGAT *F GGTCTGCACTGACGATGGTCGGCAGTATCGATAACCATGTCTATCGCGTT *F ATCGGGCCTTCACATGTTTTGCGGCGTGCGTTGAAATAGTTCGTAAAAAT *F TATACAGTAAATTCCGCTTAAGGCATGGATATAGAAGAGCTGTTCGACTG *F TTTTGATGAAGTGACGCCGTCGGTGCCGCCACCAGCACTTAACCAGGAGG *F AGAAGCCATCGGGAAGCAGTGCCTCCAAGAAAGGAAATAAGCGC # *U FBrf0183227 *a Moffat *b K. *t 2005.2.22 *T personal communication to FlyBase *u *F From: 'Kevin Moffat' *F To: r.drysdale@gen.cam.ac.uk *F Date: Wed, 08 Jan 2003 13:42:26 \-0000 *F Subject: advice on changing a gene name *F Hi Rachael, *F We've been working on a gene we termed kisir for a couple of years. *F The first allele we isolated was a male sterile \- hence we named it *F kisir (turkish for sterile). We have yet to publish any details on the *F gene except for what is in Flybase already and DNA/Protein *F accessions. We have now generated null alleles and wish to submit a *F paper shortly to the Journal of Neuroscience. HOwever the *F phenotype is one of slow peristaltic crawling. Richard Baines has *F suggested we call it slowmo, and this would be ok as it would still be *F the first publication of the gene function. I just wanted to check that *F this was an ok thing to do as far as Flybase was concerned. Any *F advice? *F Thanks, *F Kevin *F Dr K.G.Moffat *F Dept.Biol.Sci. *F Uni.Warwick *F Coventry *F CV4 7AL *F UK *F kmoffat@bio.warwick.ac.uk *F Tel \-44-(0)2476 524591 *F Fax \-44-(0)2476 523701 *F http://www.bio.warwick.ac.uk/research/moffat *F From rd120 Wed Jan 8 14:56:34 2003 *F To: r.drysdale@gen.cam.ac.uk, KMoffat@bio.warwick.ac.uk *F Subject: Re: advice on changing a gene name *F Hi Kevin, *F Since the only group that has so far published on this gene in any way *F is your own then it is possible for you to change it. I would urge you *F to do so soon and the best thing is for you to email me an explicit *F request to rename from kisir to slowmo (presumably full name would be *F slow motion) so that the name change is in place before we curate your *F paper. We'd archive your mail and change the name in the main files in *F advance of your paper, just in case anyone else published on kisir or *F used slowmo for another gene in another publication before yours comes out. *F Hope this is helpful, *F All the best. *F Rachel. *F \---------------------------------------------------------------------- *F Rachel Drysdale, Ph.D. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fly.ebi.ac.uk:7081/ *F \---------------------------------------------------------------------- *F Date: Tue, 22 Feb 2005 15:08:41 \+0000 *F From: 'Kevin Moffat' *F To: , *F Subject: Re: FB-NG Help Mail: gene data update *F Hi Rachel, *F I have a record of emailing you in February of 2003 re this very matter *F (attached) \- It was subsequently some years before we finally got the gene *F published. We would very much like to change the name to slowmo (slmo) if that *F is at all possible. *F . *F . *F . *F Thanks *F Kevin # *U FBrf0183293 *a Paladino *b E. *t 2005.3.3 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50757. *F Date: Wed, 2 Mar 2005 13:33:10 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50757 *F To: paladina@unlv.nevada.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Elana, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Genetic mapping and characterization of a gene involved in salivary *F gland glue expulsion. *F You mention a gene symbol that is new to FlyBase, drmo-o57. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, what would the full name for drmo-o57 be? Now would be a good *F time to let me know what it is, as then I can enter it in the database *F from the outset. *F With best wishes, *F Rachel. *F Date: Wed, 2 Mar 2005 17:35:24 \-0800 *F From: paladina@unlv.nevada.edu *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50757 *F Dear Rachel, *F Thank you for bringing this to my attention. The mutant (drmo-o57) is called *F drymouth o57. It has not been mapped to a single gene yet. It actually falls *F within the cytological range 66B10-66C6. There are about 20-30 genes(CG's from *F the genome project and characterized genes) that the mutant may correspond to, *F but I don't know if it would be useful to link this abstract to all of those *F genes. *F I hope this helps. Let me know if you need any other information. *F Sincerely, *F Elana # *U FBrf0183350 *a Rabinow *b L. *t 2004.12.29 *T personal communication to FlyBase *u *F Date: Wed, 29 Dec 2004 13:56:38 \+0100 *F To: matthewk@fly.bio.indiana.edu *F From: Leonard Rabinow *F Subject: incorrect link? *F Cc: patricia.uguen@ibaic.u-psud.fr, flybase-help@morgan.harvard.edu *F Hello Kathy, and hello to whoever picks this up at Flybase: *F Just to let you know: *F We obtained 2 lines which BLAST suggested should be in the same gene- *F CG3450 *F ========== *F The first line is correct, i.e. BL \#10528 IS in CG3450, via our inverse PCR. *F ===== *F The second of these lines was labeled at BL as not complementing an allele *F of wunen (a different gene): *F BL-11203 y1 w67c23; P{w+mC=lacW}l(2)k16806k16806/CyO *F l(2)k16806 *F but the BLAST with its accession number: *F AQ026117 *F said it should be in CG3450: *F http://flybase.net/cgi-bin/gbrowse_fb/dmel?name=2R:2322147..2322270;ft=blast,Bla *F st,,2322147..2322270;doexpand=1 *F The genetics is right- the hit IS in wunen (I can send our inverse PCR *F sequences if you wish), but the blast with the above sequence accession, *F which I just re-BLASTed, must be incorrect somehow- or there are 2 P's in *F the stock. The latter possibility isn't likely, at least for the recessive *F lethality, since the two above lines complement. *F I thought you might like to know. *F Best, *F Lenny *F \---------------------------------------------------------------- *F Leonard Rabinow *F Signalisation, Developpement et Cancer *F Batiment 442 bis *F Universite Paris XI *F 91405 ORSAY CEDEX *F FRANCE *F Leonard.Rabinow@ibaic.u-psud.fr *F http://www.u-psud.fr/orsay/recherche/SDC.nsf/SDC201.htm!OpenPage *F Phone: 1-69-15-74-65 *F FAX: 1-69-15-68-02 *F (from US, prefix: 011-33) # *U FBrf0183358 *a Rathke *b C. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50066. *F Date: Wed, 2 Mar 2005 13:41:51 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50066 *F To: rathke@staff.uni-marburg.de *F Cc: rd120@gen.cam.ac.uk *F Dear Christina, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F In Drosophila, both don juan and don juan like encode proteins of the *F sperm nucleus and the flagellum and both are regulated at the *F transcriptional level by the TAFII80 Cannonball while translational *F repression is achieved by distinct elements. *F You mention a gene symbol that is new to FlyBase, dj-like. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. We *F would prefer to use a shorter symbol, djl, for this gene record. Is *F that a symbol you could use? *F With best wishes, *F Rachel. *F Date: Wed, 02 Mar 2005 17:13:23 \+0100 *F From: Christina Rathke *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50066 *F Dear Rachel, *F the CG number for dj like is CG1984 and djl as short symbol is okay. *F Kind regards Christina *F Best regards also from Renate Renkawitz-Pohl *F 'Rachel Drysdale (Genetics)' wrote: # *U FBrf0183365 *a Reed *b B. *t 2005.1.20 *T personal communication to FlyBase *u *F Personal communication from Bruce Reed (reed@resultra.sickkids.on.ca) *F Date: 20th January 2005 *F The chromosome referred to as 'In(2LR)lt616-L BR27-R' in Wilk et al., *F 2004, Genetics 168(1): 281--300 was recovered from *F LS(2)ltG16//DS(2)BR27. *F DS(2)BR27 was formerly known as SDS#7-5 *F (See FBrf0064297, Reed, 1992, Ph.D. Thesis, University of Cambridge, *F England for more details). # *U FBrf0183379 *a Ribaya *b J.P. *t 2005.3.12 *T personal communication to FlyBase *u *F Date: Wed, 2 Mar 2005 13:35:58 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50855 *F To: jribaya@ucla.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Jeronimo, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Characterization of mule, a gene encoding a deubiquitinating enzyme *F required for ovary and testes germline development. *F You mention a gene symbol that is new to FlyBase, mule. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Sat, 12 Mar 2005 13:58:09 \-0800 *F From: 'RIBAYA,JERONIMO PECSON' *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50855 *F Hi Rachel, *F The gene mule mentioned in my abstract for the 46th Annual Drosophila *F Research Conference in San Diego is CG5505. I'm sorry for getting to you *F so late. Your e-mail was buried in a sea of junk mail. *F Thank you, *F Jeronimo P. Ribaya # *U FBrf0183382 *a Richardson *b H. *t 2005.2.11 *T personal communication to FlyBase *u *F Subject: RE: FlyBase query *F Date: Fri, 11 Feb 2005 17:16:06 \+1100 *F From: 'Richardson Helena' *F To: 'Gillian Millburn (Genetics)' *F Dear Gillian, *F .. concerning your queries: *F (1) cdc2-63E was a name given to this gene originally by Steve Stowers \- *F but it has now been called L63 or Eip63E (Ecdysone-induced protein 63E) *F or CG10579 *F .. *F (2) We found that l(3)S070006, l(3)S070016, L(3)02619 and l(3)L6750 *F fail to complement. And the sequence flanking L(3)L6750 has been *F determined \- BLAST searching *F with this sequence pulls out CG3874 = frc (see below), as does l(3)10098 *F which is said to be an allele of frc in flybase. So that was our logic *F \- but I guess given what you say about frc being in an intron of another *F gene it could be complicated. Looking at the current information now, *F l(3)10098 is within the 5'UTR of CG3874 whereas *F L(3)L6750 is approx 177 bp 5' to this, but so is l(3)00073 (an allele of *F frc) 177 bp 5' to this. So it is likely then if l(3)00073 is an allele *F of frc, so is L(3)L6750. We don't have data on the complementation of *F l(3)00073 or l(3)10098 with L(3)L6750 to confirm this though. So that's *F what we have. I hope that provides some clarification. *F Best regards, Helena *F ________________________________________________________________________ *F _________ *F >CG3874-RA type=transcript; *F loc=3L:complement(17396840..17398447);Feature on Genome Map *F ID=CG3874-RA; name=frc-RA; *F db_xref=FlyBase:FBtr0075228,FlyBase:FBgn0042641, *F Gadfly:CG3874-RA; species=dmel; len=1608 *F Length = 1608 *F Score = 604 bits (314), Expect = e-172 BLAST HIT on Genome Map *F Identities = 314/314 (100%) *F Strand = Plus / Plus *F Query: 177 cacactgaacatatgttccggggagaatggctgcgatttcgcgtcggtaaaaatagcaaa *F 236 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 1 cacactgaacatatgttccggggagaatggctgcgatttcgcgtcggtaaaaatagcaaa *F 60 *F Query: 237 tactcgttaatgtgctgtgggaacgcttcctccccggccccaaagtggccccgaagaaag *F 296 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 61 tactcgttaatgtgctgtgggaacgcttcctccccggccccaaagtggccccgaagaaag *F 120 *F Query: 297 tgagcaaatgtgcgcgccgcaagatagtcgccgccgaacaaacgatagtgacgaaagtga *F 356 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 121 tgagcaaatgtgcgcgccgcaagatagtcgccgccgaacaaacgatagtgacgaaagtga *F 180 *F Query: 357 tttaattcaactaccagcactcccgcaaatacgatgagtatgtcgcgcggcggcaacaca *F 416 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 181 tttaattcaactaccagcactcccgcaaatacgatgagtatgtcgcgcggcggcaacaca *F 240 *F Query: 417 actctggacttgcagccgctcctggcggagagcgatgtcggaaacagggagctggaggag *F 476 *F |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| *F Sbjct: 241 actctggacttgcagccgctcctggcggagagcgatgtcggaaacagggagctggaggag *F 300 *F Query: 477 aagatgggcggatc 490 *F |||||||||||||| *F Sbjct: 301 aagatgggcggatc 314 *F \-----Original Message----- *F From: Gillian Millburn (Genetics) mailto:gm119@gen.cam.ac.uk *F Sent: Tuesday, 18 January 2005 3:38 AM *F To: Richardson Helena *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase query *F Dear Dr. Richardson, *F I am curating your paper for FlyBase: *F Brumby et al., 2004, Genetics 168(1): 227--251 *F and I have a couple of questions. *F 1. In Table 1, one of the candidate genes you list (for the Df(3L)GN50 *F region) is 'cdc2-63E'. *F FlyBase doesn't have a record for a gene with this kind of symbol, but I *F am trying to work out if it corresponds to a gene we already have. Do *F you know which of the CG predicted gene annotations it corresponds to ? *F \- in which case I could rename the CG gene to 'cdc2-63E' in FlyBase. *F 2. on page 242, in the section where you describe the mapping of the *F 3.1 complementation group, you state that l(3)S070006 is allelic to *F l(3)L6750 and that l(3)L6750 corresponds to frc (fringe connection). *F I am wondering how you know this allelism \- I would like to be able to *F merge the 'l(3)S070006' and 'l(3)L6750' gene records in with the frc *F gene record in FlyBase, however I am a bit nervous because the frc gene *F annotation (CG3874) is located within an intron of another gene *F (CG32174) so the mapping and complementation data for these P-element *F alleles may well be complicated. *F Do you have any complementation data for 'l(3)S070006', 'l(3)L6750' and *F any particular alleles of frc or any other data which might help clear *F this up. *F Any info you give me would be recorded as a personal communication to *F FlyBase, so that users can see how we knew the information. *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- # *U FBrf0183385 *a Robertson *b H. *c K. *d Walden *e J. *f Wegner *t 2005.1.21 *T personal communication to FlyBase *u *F Date: Fri, 21 Jan 2005 15:58:25 \-0600 *F From: Hugh Robertson *F Subject: Re: Or98b *F Personal communication from Hugh Robertson, Kim Walden and Jaclyn Wegner *F Information communicated: Status of Or98b gene in D.melanogaster, *F sequencing of Or98b sequence from a number of D. melanogaster strains. *F .. *F We tried sequencing PCR *F products across the apparent single base deletion frameshift in the *F sequenced melanogaster genome from several strains. For some wacky *F reason OregonR would not amplify. In a strain called New Jersey we got *F the frameshifted sequence back. In an Ives strain we got a messy *F sequencing reaction and so went ahead and amplified from 12 individual *F flies and sequenced. The result is a nice polymorphic situation. Six of *F them are homozygous for the frameshift, three are heterozygous, and *F three are homozygous for the intact allele, which has an A present to *F encode a glutamine at the frameshift. So the intact sequence of that *F region of the gene from some alleles in the Ives strain is below (lower *F case is the next intron). *F ATGTATGCCTACTGTTTCATTACGGCTGGCCTTTCGGCCTGCCTGATGTCCCCTCTATCCATGCTGATTAGCTACCAAC *F GA *F ACAGGTGAATTGCAGCCGGAATTTCCATTTCCCAGTGTgtaagaaaaagtactgcttaatatccagaatattaagataca *F gtttctgcagATATCCCTGGGACAATATGAAGCTGTCCAACTACATCATTTCC *F I therefore believe that in addition to the evidence that this gene is *F intact in simulans, yakuba and pseudoobscura genome sequences, Or98b as *F present in the sequenced melanogaster genome has to be annotated as a *F pseudogene, but hopefully with some kind of note saying that it is *F polymorphic with intact alleles present in the species. *F .. *F Hugh # *U FBrf0183397 *a Roote *b J. *t 2005.1.24 *T personal communication to FlyBase *u *F To: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: SH1565 *F Date: Mon, 24 Jan 2005 11:09:56 \+0000 *F Dear FlyBase (Rachel), *F The allele l(2)SH1565SH1565 is a lethal allele of stc. The flanking *F sequence of the SH1565 insertion however blasts to ~5kb proximal of *F esg. *F Release 4: *F stc: 15109411..15113999 *F esg: 15329793..15332079 *F SH1565: 15335600..15335682 *F John # *U FBrf0183401 *a Rosen *b M. *t 2005.3.11 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50176. *F Date: Wed, 2 Mar 2005 13:45:45 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50176 *F To: Monika.Rosen@medkem.lu.se *F Cc: rd120@gen.cam.ac.uk *F Dear Monika, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F A putative 1-3-galactosyltransferase encoded by twiggy is required for *F growth and morphogenesis in Drosophila. *F You mention a gene symbol that is new to FlyBase, tgy. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Fri, 11 Mar 2005 10:32:33 \+0100 *F From: Monika Rosen *F Subject: Re: Helping FlyBase: ADRC-50176 *F To: 'Rachel Drysdale (Genetics)' *F Hi, *F sorry for the delay. The gene twiggy corresponds to CG7440 in the *F Flybase. *F Sincerely, *F Monika Rosen # *U FBrf0183441 *a Scott *b R. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50558. *F Date: Wed, 2 Mar 2005 13:25:24 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50558 *F To: scottrc@umn.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Ryan, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Regulation of Autophagy by TOR Signaling in Drosophila. *F You mention two gene symbols that are new to FlyBase, atg1 and atg5. *F Do you know which of the Genome Project CG annotations your genes *F correspond to? All the CGs have corresponding gene records in FlyBase *F already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F With best wishes, *F Rachel. *F From: Ryan Scott *F Subject: Re: Helping FlyBase: ADRC-50558 *F Date: Wed, 2 Mar 2005 09:58:22 \-0600 *F To: 'Rachel Drysdale (Genetics)' *F Rachel- *F ATG1 is CG10967 *F ATG5 is CG1643 *F Both were named based on sequence homology to yeast genes, and are *F published in: *F Scott et al. . 2004. Role and Regulation of Starvation-Induced Autophagy *F in the Drosophila Fat Body. Dev. Cell, 7 (2): 167-178. *F Also published in that paper are: *F ATG2 is CG1241 *F ATG8a is CG32672 *F ATG8b is CG12334 *F ATG18 is CG7986 *F I hope this helps. *F \-Ryan # *U FBrf0183448 *a Serano *b J. *t 2005.1.28 *T personal communication to FlyBase *u *F Date: Fri, 28 Jan 2005 13:01:34 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: btsz insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Julia Serano, UC Berkeley (12/04). *F P{UAS-btsz.3}3 and P{UAS-GFP.BLR}3 are homozygous viable, third chromosome *F insertions. *F P{GUS-btsz.2}2, P{GUS-btsz.1}2 and P{GUS-btsz.2poly}2 are homozygous *F viable, second chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0183486 *a Skeath *b J. *t 2004 *T personal communication to FlyBase *u *F Date: Fri, 21 Jan 2005 15:13:04 \-0600 *F To: David Sutherland *F From: Jim Skeath *F Subject: Re: spdo/tmod split *F Cc: hbellen@bcm.tmc.edu *F Dear David, *F (Hugo \- I agreed with everything you wrote but felt I should copy you anyway) *F >Dear Hugo and James, *F > *F >I'm currently engaged in the tricky task of trying to split FlyBase's current *F >record for spdo into 2 (tmod/CG1539 & spdo/CG31020), based on the data in *F >'O'Connor-Giles and Skeath, 2003, Dev. Cell 5(2): 231--243'. *F > *F >I'd like to check with you that my proposed partitioning of the alleles fits *F >with your understanding of their behaviour. Any additional complementation or *F >molecular data that could help with this partitioning would be most welcome. I *F >can curate such data as a personal communication to FlyBase. *F > *F >The tough question is what to do with 'spdoH7'(*) and its associated data. *F > *F > On the basis of the mapped insertion 'P{lacW}spdoH7' (now to be renamed *F >P{lacW}tmod)H7) it needs to be an allele of tmod. On the basis of *F >failure to *F >complement spdoC55 (see below), it needs to be an allele of spdo. The most *F >obvious explanation is that the 'H7' chromosome has mutations in both genes \- *F >suggesting I should make both alleles. This still leaves the problem of where *F >to put the phenotypic data. *F > *F >Any suggestions? *F I agree with Hugo. It most likely hits two or more genes. The only *F anecdotal light I may be able to shed is that Kate thought it might *F be a deletion \- as when she performed plasmid rescue the right rescue *F was always from tmod and the left rescue was from the original P *F insert. spdo is found between these two genes. *F As for phenotype, our lab does not know the phenotype of tmod either. *F The asymmetric division phenotype appears to arise from a defect in *F spdo. But, the phenotype of tmod is unclear to me. *F >Here is how I plan to split the rest of the alleles between the two genes: *F > *F >Alleles remaining spdo/CG31020: *F > *F >1. lesions mapped by O'Conner-Giles & Skeath: *F > *F >spdoC55 *F >spdoG104 *F >spdoK433 *F > *F >spdoZZ27 (a deficiency removing spdo/CG31020 : data from FBrf0162056 == *F >O'Connor-Giles and Skeath, 2003, Dev. Cell) *F > Strictly we only make alleles from Dfs when one of their *F >breakpoints is in the *F >gene in question \- Is this the case? *F We have not mapped the endpts of the df. But, I am fairly that *F neither of the bkpts is in spdo \- rather spdo is completely contained *F within the deficiency. *F >2. On the basis of complementation: *F > *F >spdoS097002b *F >Separable from: @P{lacW}S097002a@ *F >data from: FBrf0099763 Salzberg et al., 1997: *F >Fails to complement: spdoC55 *F >Southern analysis reveals a chromosomal rearrangement. *F >Lethality not revertible by &Dgr;2-3-induced mobilization *F > *F >Fails to complement: spdoC55 *F >Isolation of @spdoH7@ by failure to complement @spdoC55@ is described in *F >'Dye et al., '98 FBrf0102830' *F > *F > *F >Alleles to split out as tmod: *F >1. constructs: *F >spdohs.PDL is the only one of these *F >'rescue' data for 'spdoH7' \- should this now be recorded as partial *F >suppression of a spdoH7 phenotype by hs-tmod? *F > *F >2. Insertions mapped to CG1539: *F > *F >spdo02288 *F >spdo00848 *F > *F >3. Leision mapped to CG1539 *F > *F >spdoS130910 *F >A portion of the CG1539 gene is deleted. (data Dye et al., '98 FBrf0102830) *F > *F >spdoZZ27 (a deficiency removing tmod/CG1539: data from FBrf0162056 == *F >O'Connor-Giles and Skeath, 2003, Dev. Cell) *F > Strictly we only make alleles from Dfs when one of their *F >breakpoints is in the *F >gene in question \- Is this the case? *F see above. *F > *F >4. On basis of complementation only: *F > *F >spdorG347 *F >Fails to complement: spdo00848 *F >(data from BDGP) *F > *F >Thanks in advance *F I concur with what Hugo said about the origin of the confusion on this stuff. *F The only thing potentially to add is that we have identified the *F molecular lesion for a number of other spdo alleles. These data are *F in the Dev Cell paper \- if Flybase is interested in adding them to *F the listing. *F take care, *F Jim # *U FBrf0183487 *a Skeath *b J. *t 2005 *T personal communication to FlyBase *u *F Envelope-to: djs93@gen.cam.ac.uk *F Delivery-date: Mon, 14 Feb 2005 22:24:36 \+0000 *F Mime-Version: 1.0 *F Date: Mon, 14 Feb 2005 16:30:57 \-0600 *F To: David Sutherland *F From: Jim Skeath *F ...courtesy of Kate O'Conner-Giles *F Amino acid changes for spdo alleles: *F 1. G104 \- AA141 (Y to Stop) *F 2. AC81 \- AA192 (Q to Stop) *F 3. AC85 \- deletes AA202-AA336 *F 4. VV86 \- AA245 (R to Stop) *F 5. ZZ213 \- AA 264 (Q to Stop) *F 6. P46 \- AA312 (R to Stop) *F 7. Z143 \- AA415 (K to Stop) *F 8. AB153/OO3 \- AA446 (G to R) *F 9. K433 \- AA468 (S to F) *F 10. C55 \- AA504 (L to R) # *U FBrf0183497 *a Southall *b T. *t 2005.3.3 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50155. *F Date: Wed, 2 Mar 2005 13:44:28 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50155 *F To: s.terhzaz@bio.gla.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Selim, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Novel intracellular localisations and functions of SPoCk, a Drosophila *F Ca2+/Mn2+;ATPase. *F You mention a gene symbol that is new to FlyBase, SPoCk. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. Also, *F we like to record a full gene name for the symbols. SPoCk looks like an *F abbreviation \- could you let me know what it stands for? *F With best wishes, *F Rachel. *F From: Tony Southall *F To: rd120@gen.cam.ac.uk *F Date: 03 Mar 2005 14:18:24 \+0000 *F Dear Rachel, *F I am writing on behalf of Selim Terhzaz (University of Glasgow) *F regarding the gene SPoCk. I named the gene when I was doing my PhD in *F Glasgow, the CG number is CG32451. It's named SPoCk because it is a *F Secretory Pathway Calcium atpase, and it sits next to a gene called jim *F (sorry bad Star Trek joke). If you need any further information please *F contact me. I found an extra 3 transcripts which will need to be updated on *F Flybase, do we do this when we publish the paper or before? *F Best regards *F Tony Southall # *U FBrf0183526 *a Swan *b A. *t 2005.3.7 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50963. *F Date: Wed, 2 Mar 2005 13:43:05 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50963 *F To: aswan@molbio.princeton.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Andrew, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Specialized roles for Cks proteins in female meiosis, anaphase *F progression in syncitial embryros and in preventing polyploidy in *F mitotic cells. *F You mention two gene symbols that are new to FlyBase, cks30A and *F cks85A. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F With best wishes, *F Rachel. *F Envelope-to: rd120@gen.cam.ac.uk *F From: 'Swan, Andrew' *F To: ''Rachel Drysdale (Genetics) '' *F Subject: RE: Helping FlyBase: ADRC-50963 *F Date: Mon, 7 Mar 2005 10:28:17 \-0500 *F Hi Rachel, sorry for the delay. the gene we call cks30A is called Cks on *F flybase. cks85A is CG9790. they are both cks genes based on homology and *F function, and we named them for their cytological location. *F andrew # *U FBrf0183548 *a Thimmappaiaha *b R.K. *t 2005.2.4 *T personal communication to FlyBase *u CG8069 sequence annotation. *F Personal communication to FlyBase from Rajendra K Thimmappaiaha 4 Feb *F 2005 *F From: Rajendra K Thimmappaiaha *F Subject: CG8069 sequence annotation *F This is about the annotation for the gene CG8069. The database shows *F two transcripts for this gene, CG8069-RA and CG8069-RB. *F Though I don't know how the putative translation products were *F predicted, but it appears from sequence alignments of PHAX from various *F other species that the real translation start for CG8069 is \+86bp in *F CG8069-RA *F (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=24651981). *F This is further corroborated by a search for translation start *F sequences: \+86 of RA and \+74 of RB (5'-ATGATGGAAC-3') have the genuine *F translation start sites as the upstream of these starts (AAAA) match *F the consensus sequence (C/AAAA/C) for Drosophila translation start site *F (Cavener, 1987). Taking these two aspects into consideration, only *F 8069-RA can produce a full length protein (starting with \+86bp, *F 5'-ATGATGGAAC-3') as CG8069-RB is full of stops. # *U FBrf0183549 *a Thomas *b S. *t 2005.3.10 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50724. *F Date: Wed, 2 Mar 2005 13:31:22 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50724 *F To: sthomas5@utk.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Sharon, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F SNM and MOD(MDG4)56.3 are required for territory maintenance in Drosophila *F spermatocytes. *F I'm writing to check whether or not the SNM that you disuss corresponds *F to the gene record for Snm1 (FBgn0037338) in FlyBase. It seems likely *F \- but we do like to be sure. *F With best wishes, *F Rachel. *F Date: Thu, 10 Mar 2005 13:23:22 \-0500 *F From: sthomas5 *F To: 'Rachel Drysdale (Genetics)' *F Subject: RE: Helping FlyBase: ADRC-50724 *F Hi, *F Actually SNM is stromalin in meiosis formerly SA-2 or CG13916. If you need *F more info, please let me know! # *U FBrf0183574 *a Tsuda *b L. *t 2005.3.11 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50149. *F Date: Wed, 2 Mar 2005 13:43:57 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50149 *F To: ltsuda@cdb.riken.go.jp *F Cc: rd120@gen.cam.ac.uk *F Dear Leo, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Inductive activity of photoreceptor cells is regulated by *F Su(H)/SMRTER/Ebi co-repressor complex that represses taco, a Drosophila *F NRSF/REST-like molecule. *F You mention a gene symbol that is new to FlyBase, taco. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F From: ltsuda@cdb.riken.jp *F Subject: Re: Helping FlyBase: ADRC-50149 *F Date: Fri, 11 Mar 2005 10:23:53 \+0900 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F I am sorry for being late this response. We keep mentioning about taco *F in these several years, though we notice that our gene are the same as *F charlatan which Dr. Modolell's lab is working (Development 132, *F 1211-1222). So, we are going to use the name, chn, instead of taco. *F Sorry about this inconvenience for you. *F Best wishes, *F Leo # *U FBrf0183582 *a Van Vactor *b D. *c K. *d Johnson *t 2004.8 *T personal communication to FlyBase *u P{Ubi-Sara} construct and insertions. *F Date: Tue, 21 Sep 2004 10:26:23 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{Ubi-Sara} construct and insertion *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Davie Van Vactor and Karl Johnson, Harvard Medical School *F (8/04). *F The P{Ubi-Sara} construct and insertion described in Johnson et al., 2004 *F (Curr. Biol. 14:499-504; FBrf0174485) were obtained from the laboratory of *F Michael O'Connor. A 4 kb Sara cDNA fragment from clone LD13137 was blunt *F end ligated into the StuI site of pP{Ubi-CaSpeR} (FBmc0002737), a vector *F described in Brummel et al., 1994 (Cell 78:251-261; FBrf0072708). *F P{Ubi-Sara.J}2 is a second chromosome insertion. *F P{UAS-Sdc.J}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-3700 kcook@bio.indiana.edu # *U FBrf0183636 *a Wu *b C.Y. *t 2005.3.7 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50844. *F Date: Wed, 2 Mar 2005 13:34:33 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50844 *F To: wu5@buffalo.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Chia-Yen, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Modulation of polyglutamine toxicity and aggregation by dMLF and its *F interaction with 14-3-3zeta and dMADM. *F You mention a gene symbol that is new to FlyBase, Madm. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F Date: Mon, 07 Mar 2005 09:56:07 \-0500 *F From: wu5@buffalo.edu *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50844 *F Hi Rachel, *F The dMADM gene is corresponded to CG1098, and I name this gene by referring *F a paper, Lim et. al., 2002. MADM, a novel adaptor protein that mediates *F phosphorylation of the 14-3-3 binding site of myeloid leukemia factor 1. *F JBC 277: 40997-41008. *F If there is any question, please feel free to contact me, *F Chia-Yen # *U FBrf0183667 *a Yao *b S. *t 2005.3.3 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50576. *F Date: Wed, 2 Mar 2005 13:25:44 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50576 *F To: syao2@jhmi.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Shenqin, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Identification of Novel Hh Sensors Using a High-Throughput RNAi Screen in *F Drosophila Cultured Cells *F You mention a gene symbol that is new to FlyBase, iHog. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F Also, if iHog has a full name (not clear what iHog might be short for) *F then this would be a good time to tell me so I can get it into the *F database. *F With best wishes, *F Rachel. *F Date: Thu, 03 Mar 2005 15:14:02 \-0500 *F From: shenqin yao *F Subject: Re: Helping FlyBase: ADRC-50576 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F The iHog gene corresponds to CG9211 in the Genome Project CG *F annotations. It is short for interference hedgehog since iHog was *F identified as a novel Hedgehog pathway component in a genome-wide *F screen utilizing RNA interference. I hope this information helps. And *F please don't hesitate to contact me if you need more information about *F iHog. *F Best regards, *F Shenqin # *U FBrf0183671 *a Yonekura *b S. *c C.H. *d Lee *t 2005.2.28 *T personal communication to FlyBase *u Annotation of the DN-Cadherin (CadN) gene. *F From: 'Yonekura, Shinichi (NIH/NICHD)' *F To: ''flybase-help@morgan.harvard.edu'' *F Cc: 'Lee, Chi-hon (NIH/NICHD)' *F Subject: annotation of the DN-Cadherin (CadN) gene *F Date: Mon, 28 Feb 2005 12:47:07 \-0500 *F To whom concerns *F We would like to submit an error report regarding the annotation of the *F DN-Cadherin (CadN) gene. The current annotation denotes three alternatively *F spliced exon modules (exons 7a/b; 13a/b; 18a/b). Our RT-PCR analyses using *F embryonic and larval RNA as templates have revealed an additional exon, *F designed exon 7a' (with the sequence GGTTGTTATCCA). This sequence was only *F found in the transcripts that contain the exon 7a (but not those with exon *F 7b), and it encodes for four amino acids without interrupting the phase of *F the original reading frame. The exon 7a' was mapped to a genomic sequence *F located between exon 6 and exon 7a. By combinatorial use of these *F alternative exons, the CadN gene is capable of generating 12 isoforms (=3 X *F 2 X 2; exons 7a/7a,a'/7b; 13a/13b; 18a/18b). These findings were recently *F published (Development, 2005, 132: 953-963). *F Sincerely yours, *F Shinichi Yonekura and Chi-Hon Lee *F Unit of Neuronal Connectivity *F Laboratory of Gene Regulation and Development *F National Institute of Child Health and Human Development *F National Institutes of Health *F Building 18T, Room 106 *F MSC 5431 *F Bethesda, MD 20892 *F Tel: 301-435-1940 *F Fax: 301-496-4491 *F e-mail: leechih@mail.nih.gov *F http://eclipse.nichd.nih.gov/nichd/lgrd/unc/index.htm # *U FBrf0183679 *a Yucel *b G. *t 2005.3.2 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50649. *F Date: Wed, 2 Mar 2005 13:28:22 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50649 *F To: gy242@nyu.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Gozde, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Role of Blimp1 in anterior-posterior patterning of Drosophila melanogaster. *F You mention a gene symbol that is new to FlyBase, Blimp-1. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best wishes, *F Rachel. *F From: Gozde Yucel *F To: 'Rachel Drysdale (Genetics)' *F Date: Wed, 02 Mar 2005 13:09:25 \-0500 *F Subject: Re: Helping FlyBase: ADRC-50649 *F The CG number is CG5249 and the gene is called Blimp-1 in mammals. *F Thanks.. # *U FBrf0183693 *a Zhong *b L. *t 2005.3.3 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50512. *F Date: Wed, 2 Mar 2005 13:22:55 \+0000 (GMT) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: ADRC-50512 *F To: lzhong@syr.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Lei, *F We are currently curating the abstracts for the upcoming 46th *F (San Diego) Annual Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Spermatogenic expression patterns and male-sterile phenotypes associated with *F testis-specific proteasome subunit genes. *F You mention gene symbols that are new to FlyBase, Pros&bgr;5R1 and *F Rpt3R. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F With best wishes, *F Rachel. *F Date: Fri, 4 Mar 2005 10:23:57 \-0500 *F From: Lei Zhong *F To: 'Rachel Drysdale (Genetics)' *F Subject: RE: Helping FlyBase: ADRC-50512 *F Dear Rachel, *F The CG number of beta5R1 and Rpt3R are: *F beta5R1:CG9868 *F Rpt3R:CG9475 *F Thank you. *F Lei # *U FBrf0184335 *a Ryder *b E.J. *t 2004.4.26 *T personal communication to FlyBase *u Exelixis CG deletion data. *F File deposited: ExelData.txt ; File date: 2005.4.13 ; File size: 515147; File format: txt # *U FBrf0184722 *a Bellen *b H. *t 2005 *T personal communication to FlyBase *u *F Hugo # *U FBrf0188390 *a Ferrus *b A. *t 2005.4.22 *T personal communication to FlyBase *u *F From: Alberto Ferrús *F To: Chihiro Yamada *F Subject: Re: FlyBase Query (ADRC05 1020C) *F Date: Fri, 22 Apr 2005 16:06:33 \+0200 (15:06 BST) *F Dear Dr. Yamada: *F We are about to initiate writting a manuscript describing the case of the *F two Frequenin genes. In any case, let me give you a short summary of the *F situation. It seems that some changes will have to be introduced in the *F current data base. Key points are: *F 1) There are two Frequenin genes, Frq 1 and Frq 2, that correspond to *F CG5744 and CG5907 respectively. *F 2) Frq1 and Frq2 have almost the same aminoacid sequence except for 10 *F residues. Some phenotipic differences will be shown in the upcoming paper. *F 3) As indicated in the database, there is a small gene between the two *F Frqs, CG12611, that we intend to name 'Andorra'. *F Hope this is helpful, *F Cheers, *F A. *F Alberto Ferrús *F Instituto Cajal *F Ave. Dr. Arce 37 *F Madrid 28002, Spain *F ph.: \+34 (9)1 585 4738 *F FAX: \+34 (9)1 5854754 *F http://www.cajal.csic.es/departam/depneurob/ *F From: Chihiro Yamada *F To: AFERRUS@CAJAL.CSIC.ES *F Cc: Chihiro Yamada *F Subject: FlyBase Query (ADRC05 1020C) *F Date: Fri, 22 Apr 2005 13:28:44 \+0100 *F Dear Dr Ferrus, *F I am currently curating the Program Addendum to the 2005 ADRC *F conference. I have a question which I was hoping you could answer for *F me. In this abstract: *F You describe a new gene \- frq2 \- do you know which gene model (CG *F number) that this gene corresponds to? If not, can you give me any other *F information you have on its cytological location? *F Any new information you give us will be curated as a personal *F communication from you to FlyBase, if that's fine with you. *F Best wishes, *F Chihiro *F \-- # *U FBrf0188415 *a Brennecke *b J. *t 2005.7.3 *T personal communication to FlyBase *u *F From: Julius Brennecke *F To: cy200@gen.cam.ac.uk *F Subject: Re: Fwd: FlyBase Query (cy2593) *F Date: Sun, 3 Jul 2005 16:15:05 \+0200 (15:15 BST) *F Dear Chihiro, *F here is the requested info. *F the mir-2b construct is based on miR-2b-2 (the miRNA in the spitz intron). *F the miR-6 construct was obtained from Eric Lai as indicated in the *F paper and it *F contains all miR-6 hairpins as they are clustered in the genome. *F i'll be happy to answer any further questions. *F best, *F julius *F >> Dear Dr Cohen, *F >> *F >> I am currently curating your paper for FlyBase: *F >> *F >> Brennecke et al., 2004, PLoS Biol. 3(3): 404--418 *F >> *F >> I have a couple of quick questions I was hoping you could answer for me: *F >> *F >> mir-2b *F >> ====== *F >> You make a UAS construct using mir-2b sequences. However we have two *F >> genes in our records mir-2b-1, mir-2b-2. Could you tell me which one of *F >> these you used to make your construct? *F >> *F >> mir-2b-1 *F >> CR32990 *F >> 29A1 *F >> AE003620 *F >> AJ421759 *F >> MIR:MI0000119 *F >> *F >> *F >> mir-2b-2 *F >> CR32964 *F >> 37F2 *F >> AE003663 *F >> AJ421760 *F >> MIR:MI0000120 *F >> *F >> mir-6 *F >> ====== *F >> Likewise with mir-6. You make a UAS construct using mir-2b sequences. *F >> However we have three genes in our records that this could be. Could *F >> you tell me which one of these you used to make your construct? *F >> *F >> mir-6-1 *F >> CR33004 *F >> 56E1 *F >> AE003795 *F >> AJ421764 *F >> MIR:MI0000124 *F >> Rfam:RF00143 *F >> *F >> mir-6-2 *F >> CR33040 *F >> 56E1 *F >> AE003795 *F >> AJ421765 *F >> MIR:MI0000125 *F >> Rfam:RF00143 *F >> *F >> mir-6-3 *F >> CR33039 *F >> 56E1 *F >> AE003795 *F >> AJ421766 *F >> MIR:MI0000126 *F >> Rfam:RF00143 *F >> *F >> *F >> Best wishes, *F >> *F >> Chihiro # *U FBrf0188435 *a Hsiung *b F. *t 2005 *T personal communication to FlyBase *u *F Date: Fri, 23 Sep 2005 11:49:31 \-0700 *F To: c.yamada@gen.cam.ac.uk *F From: Frank Hsiung *F Subject: Re: Fwd: FlyBase Query (cy2646) *F Dear Chihiro, *F The Vav gene that we used to construct the Vav-GFP described *F in Fig 5 c,f is indeed VAV1 (GDB:127112). It was cloned into the *F pUAST vector, and expressed as clones under the control of a hsFlp; *F abxubs>FRT>Gal4 driver. *F Hope this helps. *F regards, *F Frank Hsiung *F >Dear Dr Kornberg, *F > *F >I am currently curating your paper for FlyBase: *F > *F >Hsiung et al. 2005, Nature 437(7058) 560--563. *F > *F >I have a quick question I was hoping you could answer for me. *F > *F >Vav-GFP *F >======= *F >In Figure 5 c,f you show Vav-GFP labelled clones. *F > *F >I believe the Vav gene you are talking about is called VAV1 by GDB *F >(GDB:127112). Can you confirm this? Secondly, could you givw me some *F >molecular details about this construct? e.g. What promoter drives *F >expression of the Vav-GFP? *F > *F >Best wishes, *F > *F >Chihiro # *U FBrf0188442 *a Roeper *b K. *t 2005 *T personal communication to FlyBase *u *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Katja Roeper, University of Cambridge (9/05). *F P{UASp-GFP.Act79B.R291H}30-1 and P{UASp-GFP.Act87E}12-1 are *F homozygous and hemizygous viable and fertile, X chromosome insertions. *F P{UASp-GFP.Act79B.R291H}2-2, P{{UASp-GFP.Act79B}3-1, *F P{UASp-GFP.Act42A}5-5, P{UASp-GFP.Act88F}15-1, P{UASp-GFP.Act57B}6-3 *F and P{UASp-GFP.Act5C}2-1 are second chromosome insertions. *F P{UASp-GFP.Act79B}2-2, P{UASp-GFP.Act87E}7-6, P{UASp-GFP.Act42A}1, *F P{UASp-GFP.Act88F}1-2, P{UASp-GFP.Act57B}10-2 and P{UASp-GFP.Act5C}3 *F are third chromosome insertions. # *U FBrf0188448 *a Gergen *b P. *t 2005 *T personal communication to FlyBase *u *F Date: Thu, 6 Oct 2005 09:10:01 \-0400 *F To: David Sutherland *F From: pgergen@life.bio.sunysb.edu *F Subject: Re: FlyBase Curation query *F David, *F There is one 'wild-type' UAS-runt construct that we made that is *F distributed. The first publication to mention this is the Li and *F Gergen paper, although thework in the Tracey et al paper really *F provides the detailed characterization of this construct in the *F context of using the maternal GAL4 (NGT) constructs that were also *F developed in the lab. Hope this clears things up. *F Peter Gergen *F >Dear Peter, *F > *F >Do you have time to help me clear up a question I have about constructs *F >generated in your lab? *F > *F >We currently have 2 separate records for UAS-runt constructs made in *F >your lab. *F >These come from our curation of the papers: *F > *F >FBrf0110190 == Li and Gergen, 1999, Development 126(15): 3313--3322 *F >& *F >FBrf0123220 == Tracey et al., 2000, Genetics 154(1): 273--284 *F > *F >It struck me as unlikely that you would have made the same construct twice in *F >your lab, especially within such a short span of time. Are they the same *F >construct, or is there some distinction? # *U FBrf0188480 *a Tupy *b J. *t 2005.4.22 *T personal communication to FlyBase *u *F From: Jon Tupy *F To: Sima Misra *F Cc: 'Gillian Millburn (Genetics)' , Madeline Crosby *F , Jon Tupy *F Hi Gillian, *F Here is a table of the 14 new ncRNAs from our paper: *F id symbol *F CR33327 pncr001:3R *F CR33938 pncr002:3R *F CR31696 pncr003:2L *F CR33939 pncr004:X *F CR33946 pncr008:3L *F CR33940 pncr009:3L *F CR33943 pncr010:3L *F CR33947 pncr011:3L *F CR32957 pncr012:2L *F CR33941 pncr013:4 *F CR33944 pncr014:3L *F CR33948 pncr015:3L *F CR33942 pncr016:2R *F CR33945 pncr017:3R *F Per BLASTP analysis by Lynn Crosby, please change the gene symbols for *F these records to the following: *F uniquename | symbol | type *F \------------+---------+------ *F CG30425 | RpL41 | gene *F CG15304 | Neb-cGP | gene *F CG6503 | CG6503 | gene *F Best, *F Jon # *U FBrf0188490 *a Myrick *b K.V. *c F. *d Huet *e S.E. *f Mohr *g I. *h Alvarez-Garcia *i J.T. *j Lu *k T.M. *l Martin *m M.A. *n Crosby *o W.M. *p Gelbart *t 2005.5.16 *T personal communication to FlyBase *u Deletion Generator Project genomic insertion collection. *F Deletion Generator Project genomic insertion collection *F Myrick KV, Huet F, Mohr SE, Alvarez-Garcia I, Lu JT, Martin TM, Crosby MA, *F Gelbart WM. *F Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA *F An extensive collection of autosomal insertions of the deletion-generator *F element P{wHy} has been generated and made available through the Bloomington *F Drosophila Stock Center (list appended). *F The identifying numbers for insertions in this collection have been *F revised. The following previously published insertions have been *F assigned new designations as indicated: *F previous new *F P{wHy}01D01 P{wHy}DG01401 *F P{wHy}01D09 P{wHy}DG01409 *F P{wHy}CG499614H10 P{wHy}CG4996DG14810 *F P{wHy}CG637002B10 P{wHy}CG6370DG02210 *F P{wHy}eIF3-S814F06 P{wHy}eIF3-S8DG14606 *F The DG16211 had been mismapped. The insertion falls in a dicistronic *F gene; this dicistronic overlaps the 3' UTR of a gene on the opposite *F strand. *F The 8bp repeat of the DG16211 insertion overlaps the last 2 nt's of the *F stop codon for CG33672 (one of the dicistronics). *F It is also in the 5' UTR for CG33671 (second of the dicistronics). *F It is also in the 3' UTR of spt4 (on opposite strand). *F File deposited: Myrick.2005.5.16.txt ; File date: 2005.5.17 ; File size: 19291 *F ; File format: txt # *U FBrf0188500 *a Burtis *b K. *t 2005.2.5 *T personal communication to FlyBase *u FlyBase error report for CG1211 on Mon May 2 23:36:15 2005. *F Date: Mon, 2 May 2005 23:36:15 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: kcburtis@ucdavis.edu *F Subject: FlyBase error report for CG1211 on Mon May 2 23:36:15 2005 *F Error report from Ken Burtis (kcburtis@ucdavis.edu) *F Gene or accession: CG1211 *F Gene annotation error *F Gene CG1211 should be split. *F Comments: Dear curators, *F Would any of you be willing to entertain the possibility that CG1211 is *F actually two genes joined in the annotation ? It is clearly a duplicated *F region, and there seem to be two possibilities: either the duplication *F resulted in a polypeptide with tandem domains, or the duplicated/diverged *F copies have been annotated into a single gene. There doesn't seem to be any *F definitive cDNA evidence. *F I must admit that I haven't yet tried to use Apollo to figure this one out. Is *F it possible to make two good genes with two good ORFs ? I would guess that the *F methionine at position 354 in NP_612083 might be the beginning of the second *F ORF. *F Thanks for your thoughts, *F Ken # *U FBrf0188501 *a Texada *b M. *t 2005.4.26 *T personal communication to FlyBase *u FlyBase error report for CG31732 on Tue Apr 26 14:03:23 2005. *F Date: Tue, 26 Apr 2005 14:03:23 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: mtexada@bioc.rice.edu *F Subject: FlyBase error report for CG31732 on Tue Apr 26 14:03:23 2005 *F Error report from Michael Texada (mtexada@bioc.rice.edu) *F Gene or accession: CG31732 *F cDNA or EST error *F Comments: I have sequenced the 3' ends of many of the EST's available for this *F gene... *F i've removed the vector sequences, and here is the data: *F >AT19027.3prime *F TATTTTTTTTTTTTTATCGTTTTAGATATTAAAACACACCCTAGTTTTTCCATTTAGACATTAAGTTCCGCTCAATGTG *F AGCATAAAATTTGGGGAAAACATTTTTAATGAAATGATCCTGGACAATCAAATACCCCCATATGGATTGGCCACCGGGT *F TGCCAATTTCCGCGCGAATTCCTAAATCAAGGGATTGCAGAACTGATCGTTCAAGGCTGATTCTGGTAAAAATCCGTGC *F GAGGGCTGGTCAAGTTCTGCAATCGCTGGCCATTTGTTCCCCGCCTCGGGAGTGGCCAACCGCCACCCCAGTGGACCGC *F CTGCTCCACTCGTTTTACTCCCGCCTCCAAGAGTTGTATTCCGGAAAAGTATACGAAAGTCGATGGTTTTCTCGGCCCG *F CTTGGCGCAAAAGAACCATCGCCCCTCCGCCCTTAACGGGGGGGGCCTTTAATTTCCAAAGGGAGTCAATAATCGTTCC *F CGGCGGGGTAACTCCGCGCTGTTATTAAAGCGGGATTCGATTTTGGGGCAATTTCGAAATGGGCGAACCCCTCCCGCGA *F AATTCAGGTGCTCTTCCACGGTGGAACTTTCCGGAGCCGAGATACTCCCGTCGAGAGGCTCTGCTATTTCCGAACAGAT *F GCCCCTTTTTCCTCTCCCATGCGGGAAAACCCCTCTCTCCTCAAAGGAACACCCGGGTAAACAAGACCAGGCGGTGTGG *F GCGCTCGGCCTTCTTGGCCCCTGTACACTCGGAGAATTCCCAAGCGCACGGTTTCTCTATGGGGATACCCCCTATTTTT *F TTGTTTTTCCAGGAGGAGTCCTCTGGGGGGAGAAACATATCTCTGTGTGAGAGAGCCTCTCTTCGGCCATATCTCTCCA *F CAGAAAGAAATATTGTGTGACGGCGGCGAGGTGCTGTGTCTCCTCTCTAAGTCTCTCTCCCCTCTTTGTTGGCGCGAGA *F GGAGGATAGGGTTTTTTTTGTATTTTTTTTTTACACAAAAACTATTTCCTTTAATAAAATTCATAAAAAAAAGGGGGCG *F GGAAAATGAAAAAAAAAAATC *F >AT03435.3prime *F TTTTTTTTTTTTTTTTTTTTTACACAAGCACTTAGTTTTTATTTTATACCACAATTTACAAAGACAATTTACAAGTTAC *F GCGCCTCCAAAGTGTTGATGGCATAGAGATTTTGGGTGAGAACGATTTTTAAATGGAAGGAGGAGTTGCATGCAAATCC *F TGGCGACTCCTACGACTGATCCTGTGACGATCGAGAACGCCGCAATATGGATTGGGCCACCGGTTGCCCAATTCCAGCC *F GGACTTCCTGAGATCAAGGAATTGCAGTACTGATCGTTCATAGTCTGATTCTGGTTAAAAATCCGCTGCGAGGTGCTGG *F TCAAGTTCTGCAATCGCCTGGCCATATCGTTCCCCGCCTCGGTATTGGTCAGACCGCCACTCTTCGGAGGTGGGCAACT *F TATCGCCGAACTGGACGCGCAGTTGACCGCATGCTTCACTCGTTTTATCTCCGTCTCCAAGAGCTTGTAGTCTCGGAAT *F AGATTGCGATAGTCCTCGGCCAACGTCCGGTTTTCGTCCAGCAGCATGTGGATGTGTTCCTCGGCCCGCTTGGCGCAAA *F GACGCATCGCCTCATCGGCCCTAACCTGGGCGTCCTTCCATTTTCGAAAGGAGTCGATAATCTGTTCCTGCTGCTGGTT *F AACTTCGGTCTGTTGATTTAAGCGGATCTGCATTTGCTGGCAATTTTCCGAAATGGTACGCAACTCCTCCCGCAGAGAT *F TCAGTTGTCTCGTTCATCCGCTGGATACTTTCGCGATGCCGATGTCATCCAGTTGCAGATGGCTCTGCATTTTCCGAAC *F AAAGTGCGCATCTTTTCCATCTCCAACTGGGCACTCTTTTGTTGCTCTTGAAGTGCCGCTCTCGGCCGAGACCAGCCCC *F TCTCTTCAAGCGACCACCTCCTCCGCAAATTTGCCCTTGTTTCGTCATCTGCACTGTCACTGATCAGTCCGTTGCAGCT *F CGCCTTCCTTGGCTCTGACCTGCCTGATCCGACTGCCTAGCTTGTTCTTGCAATCCCTTGATTCGGTTCCAAGTGATCC *F GAATGCGAAAACACTCTTTGAATCTCTTTGAAAGTCCGAACGACTTGTAGGGGCGCGAAGTACACTTATCCGCCTTTAA *F GGAGTTTAGCGTTCCCACGGTACGGTTCGGACATATTGGAACCCA *F >AT15480.3prime *F TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCAACCCCTTATTTTTTTTTTTTTA *F CCCCAATTTACAAAAACAATTTACAAGTTACCCCCCCCCAAAGTGTTGATGGCATAAAAATTTTGGGGGAGAACAATTT *F TTAAATGGAAGGAGGAGTTGCATGCAAATCCTGGCGACTCCTACAACTGATCCTGTGACGATCGAAAACCCCCCAATAT *F GGATTGGGCCACCGGTTGCCCAATTCCAGCCGGACTTCCTGAAATCAAGGAATTGCAGTACTGATCGTTCATAGTCTGA *F TTCTGGTTAAAAATCCGCTGCGAGGTGCTGGTCAAGTTCTGCAATCGCCTGGCCATATCGTTCCCCGCCTCGGTATTGG *F TCAAACCGCCACTCTTCGGAGGTGGGCAACTTATCCCCAAACTGGACGCGCAGTTGACCGCATGCTTCACTCGTTTTAT *F CTCCGTCTCCAGAGCTTGTAGTCTCGGAATAAATTGCGATAGTCCTCGGCCACGTCCGGTTTTCGTCCAGCACATGTGG *F ATGGTTTCTTCGGCCCGCTTGGCGCAAAACCCATCCCCTCATCGGCCCTACCTGGGCGTCCTTCATTTTCAAAAGAGTC *F AAATCCTG *F >AT25733.3prime *F TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTAAACCCCAATTTTGCAAAACA *F ATTTTCAAGTTTCCCCCCCCCCAAGTGTTGATGGGATAAAAATTTTGGGGGAAAAAAATTTTTAAAAGGAAAGAGGAAT *F TGCCTGCAAATCCTGGCGACCCCCTGCACTGATCCTGTGACAATCGAATTCCCCCCAATATGGATTGGGCCCCGGGTTG *F CCCAATTCCCGCCGGAATTCCGGAGGTCAAGGAATTGCGTCCCGGAATCTTCATTCTCTCAATCCGGGTAAAAATCCCG *F TGCGAGGGGCTGGCCAAGTTCCTAATGACCCTGGGGTTATGGGTCCCCCCCTCGGTATTGTGGAAACCCCCGCCCTTGC *F GAGGGGGGCAAGATTTCCCCAAATCGGAGCCCAAGGTAACCGCCGCCTTGCCCTGTTTTTCCCCCCTCACCAAAAACGT *F GTACTGATCGAGTAAAATCCAAGATGACGCGCGGCACTGTCGCTTCTTCTCCAGCACCATGTGGATGTGTTCCTCCGCA *F CGCGTGGCGCAAAAATCATCTACCGCAACCGACCCATACCTGGGATCGCTTCTGGTTTTGAAAAGAATCCACAATCTAG *F TCCCGGTCGCGGTTCATTTTCGGGCTGTTTATTTAGGGAGGTATCGGTCTTGCTTGGCGATTTTCCGAAATGGTGACGC *F AACTTCCCCGCAGAGATCATTTTGGCTCGTCTGCCTCTGGAAACTTACGGCATCCTGATGTTGCCACTGGGCAAGGATC *F TGCCAATCCCAGAAAAATGGCCATCTTTTCTCCTCAATTGGGGCCTCTTTTGTGTATATTGAAATGAAGCTTGGGCG *F >GM26777.3prime: *F CATATCGCTTGTATCGCGTCCGCGGTCAGTGTATCGTTGGGCGATTCCTCCATTTTGGCCTGATCTCTGGACTCAATGC *F ACCGGGTGATCTCGTGCAGCTGTTGATTGAGCTGTGCTATGCACTGATCCTTCAAAAGTAACTTCTGCCTGGCCGTGTG *F TACAACCTCGTCCAAGGCATTGATCTGTGACTTCAGTTCGCCGATCTCGTCATGCTTGGCGGCTATCTCGCCGACTAAG *F GACTTCATGCTCTCGGACATCTTCTCGCTCTCGCATTCCGCGTGGGCGGGATCCTTTCCCACGCCCCGCAATTGATTAA *F CGGTCAGTGTCTCTAGGGAAATGCCATTGAAATCAAAGTCGGTGCAGTGAGGAGAATGCTGCACGCGTTCTATGATCGC *F ACAAATGTCCTCACGCAGCCTACGATTCTCCTCGAGGGTATGCTGTTGTCGCACTTGGACAGACTGACAGCAGGCAACG *F ATCTTGCTGTACGCCTCCTTCTCCAAGTCCGACACGTTGCCCAGGATCACGCTGTTCACGTGGAGCAGGAAATCCACCC *F ACTCCTGGAAAGTGATCAGACGCAACTGGCAAGTGTGCATGTCATCACCCTCGTGCTCATCTAGGCAGTCAATCTCGTC *F CAGCCGCTTCAGAAATATGCTGTTCAGCTCAAAGCTCATATCGCCAGCATCGCCGTTGGTATCCCGCAGCTTCTGGATC *F AGACAGCGGTAATGTTCGGTGGACATCGCCTCGGTGCTGGCAGTGCACCGATCAGGAGATGAGCTTTTAGCGTCGATTC *F TTGATGTGGATGCGAGGCACTCGTTCCGGTTGGCTGCCCTTTCCGACACATTTGACTGCCAGATGATGGTGGTGGCGGT *F GGCGATGATGCAGTCTCGACGGATTCAACCATGGACTGGCGTCTAGGAGAAACGCTTGGCGATGGCCATGAATCATTCA *F AAGTATTACCCTCCTCCTTATAGACTGAATTTTCGCAAGATAGCTTGGTATTACTGATGACTTAGTTCCATTAATCATT *F AACGGATCAGGCATGATGGAAATAAG *F >GM26781.3prime *F CATATCGCTTGTATCGCGTCCGCGGTCAGTGTATCGTTGGGCGATTCCTCCATTTTGGCCTGATCTCTGGACTCAATGC *F ACCGGGTGATCTCGTGCAGCTGTTGATTGAGCTGTGCTATGCACTGATCCTTCAAAAGTAACTTCTGCCTGGCCGTGTG *F TACAACCTCGTCCAAGGCATTGATCTGTGACTTCAGTTCGCCGATCTCGTCATGCTTGGCGGCTATCTCGCCGACTAAG *F GACTTCATGCTCTCGGACATCTTCTCGCTCTCGCATTCCGCGTGGGCGGGATCCTTTCCCACGCCCCGCAATTGATTAA *F CGGTCAGTGTCTCTAGGGAAATGCCATTGAAATCAAAGTCGGTGCAGTGAGGAGAATGCTGCACGCGTTCTATGATCGC *F ACAAATGTCCTCACGCAGCCTACGATTCTCCTCGAGGGTATGCTGTTGTCGCACTTGGACAGACTGACAGCAGGCAACG *F ATCTTGCTGTACGCCTCCTTCTCCAAGTCCGACACGTTGCCCAGGATCACGCTGTTCACGTGGAGCAGGAAATCCACCC *F ACTCCTGGAAAGTGATCAGACGCAACTGGCAAGTGTGCATGTCATCACCCTCGTGCTCATCTAGGCAGTCAATCTCGTC *F CAGCCGCTTCAGAAATATGCTGTTCAGCTCAAAGCTCATATCGCCAGCATCGCCGTTGGGTATCCCGCAGCTTCTGGAT *F CAGACAGCGGTAATGTTCGGTGGACATCGCCTCGGTGCTGGCAGTGCACCGATCAGGAGATGAGGCTTTTAGCGTCGAT *F TCTTGATGTGATGCGAGGCACTCGTTCCGGTTGGCTGCACTTTCCGACCCATTTGACTGGCAGATGATGGTGGTGGCGG *F TGGCGATGATGCAGTCTCGACGGATTCAAACCCATGGACTGGCGTCTAGGACGAACGGCTTGGCGACTGGCCATGATAT *F CAATCAAATGTATTACCACGTTCCTCTTACTAAAACTGATTGATCGCAAGGAATACTTGTGTTATGACTGATTGACTTA *F AGTCCATCAACTCTCAAGGCCACCAGCCGCTATGGGAAAAGGGCTTTTCGC *F >RE12523.3prime *F AATACTGGAGAGTTTTTTTTTTTTTTTTGGTTGGGATATGAAAGAAACTTTATTTAAAACTAGTTCATTTCATTAAAAC *F TATTCATTTCAGCAATCTGTGATTCTAGTTTCTGTTTAACCATATATAGATAGATATAGATTTGAGTGGAGTTGAGTTT *F TCGTGGCGAACAATCGCGATGATAAATAACTTAAGAACGACAAACTTTAAGCTAAACTATACAAACTTCTTAACGTGGC *F TTAGATTAGACTAACTGTTTAGGAGGCCCAACAAGTGACATAGTTACTTGGAGGTCCTAAACGTTGTAGTTGTGTTTTT *F CGTTGGTATAGTTTTAGTTGTCGTTGGCTTGTTTGAAGGTTTAGGTGTTGGTTGTTGTTTGTTTGTTTTTGCCGTGGCT *F CCCGGGATTTTGGTGGTTTTGGTTGGCTTCCTTGTTTGGGAAGGTGTTTTCGTATCGCATTCAATTTCCACTTGATTTT *F GCTCCTGCTCGAATTTCCGTGCAAACATATCCGCAATGGTCGTACAAAATGGATTTTCTTTCGAGGATTCGTCGTAGAT *F TTCGTTGTAGGAGAACTCCTTGGAACCCAACGAGCAGATGAGTTCGGCGATCTCCCTTAGAACATCGCTATTTCTTTGA *F AGAACGCTGCTTTCCGATTCAATCCTCGCCTCCAGCTCTGAAATTTTGATTTTCGACTCGGTGTCCAGGCGCTGGTACT *F TTCCGTGCAGTTCTCGATTAAGTTGGCTAAGGTTGAGCAGGCGTCTTACGTAGAGCTTCCAGCATCTGAGCATCCGCGT *F CCAGATCAAAGGCCCTGATTCGGATAACGGAAATTAGCAGATTGATCGAGTCTGACTGAGATTGTCAAAGAATACAATG *F CCTCGAGTTTTCCGAGGTCCTCACCTCGGAACATTTTCATAACAGGAACAGTTTCCGATCATGTCGATCTGTTTGTTGC *F AATAGAAAATTTGATCCGTTAGACCGCAATCTCTGGGTTCGGATAAAAATCCGACTCCTGTACGGCTCGGGGTATTTGG *F ATTC *F >RE13793.3prime *F AATACTGGAGAGTTTTTTTTTTTTTTTTGGTTGGGATATGAAAGAAACTTTATTTAAAACTAGTTCATTTCATTAAAAC *F TATTCATTTCAGCAATCTGTGATTCTAGTTTCTGTTTAACCATATATAGATAGATATAGATTTGAGTGGAGTTGAGTTT *F TCGTGGCGAACAATCGCGATGATAAATAACTTAAGAACGACAAACTTTAAGCTAAACTATACAAACTTCTTAACGTGGC *F TTAGATTAGACTAACTGTTTAGGAGGCCCAACAAGTGACATAGTTACTTGGAGGTCCTAAACGTTGTAGTTGTGTTTTT *F CGTTGGTATAGTTTTAGTTGTCGTTGGCTTGTTTGAAGGTTTAGGTGTTGGTTGTTGTTTGTTTGTTTTTGCCGTGGCT *F CCCGGGATTTTGGTGGTTTTGGTTGGCTTCCTTGTTTGGGAAGGTGTTTTCGTATCGCATTCAATTTCCACTTGATTTT *F GCTCCTGCTCGAATTTCCGTGCAAACATATCCGCAATGGTCGTACAAAATGGATTTTCTTTCGAGGATTCGTCGTAGAT *F TTCGTTGTAGGAGAACTCCTTGGAACCCAACGAGCAGATGAGTTCGGCGATCTCCCTTAGAACATCGCTATTTCTTTGA *F AGAACGCTGCTTTCCGATTCAATCCTCGCCTCCAGCTCTGAAATTTTGATTTTCGACTCGGTGTCCAGGCGCTGGTACT *F TTCCGTGCAGTTCTCGATTAAGTTGGCTAAGTTGAGCAGCGTCTACGTAAGCTTCAGCATCTGAGCATCGCGTCCGATT *F ACAGGGCCTGATTCGGATCACCGAGATTAGGCAGGATGATCGAGTTTGACTGAGGATTGCCAAGAATCATGCCCTCGAG *F TTTTTCGAAGGTCCTCCGCTCGATACATTTCGATAAACAGACCCGTTTCCCATCCAGGTCTGAATCGGTTGTGTGCATG *F AAATTTGATCCGTTAACCCGCCATCCCCTGGGTCTGGACAGATTCCCATCTGTACCCCCCCGGGGTACTTGGAATCC *F >RE16347.3prime *F AATACTGGAGAGTTTTTTTTTTTTTTTTGGTTGGGATATGAAAGAAACTTTATTTAAAACTAGTTCATTTCATTAAAAC *F TATTCATTTCAGCAATCTGTGATTCTAGTTTCTGTTTAACCATATATAGATAGATATAGATTTGAGTGGAGTTGAGTTT *F TCGTGGCGAACAATCGCGATGATAAATAACTTAAGAACGACAAACTTTAAGCTAAACTATACAAACTTCTTAACGTGGC *F TTAGATTAGACTAACTGTTTAGGAGGCCCAACAAGTGACATAGTTACTTGGAGGTCCTAAACGTTGTAGTTGTGTTTTT *F CGTTGGTATAGTTTTAGTTGTCGTTGGCTTGTTTGAAGGTTTAGGTGTTGGTTGTTGTTTGTTTGTTTTTGCCGTGGCT *F CCCGGGATTTTGGTGGTTTTGGTTGGCTTCCTTGTTTGGGAAGGTGTTTTCGTATCGCATTCAATTTCCACTTGATTTT *F GCTCCTGCTCGAATTTCCGTGCAAACATATCCGCAATGGTCGTACAAAATGGATTTTCTTTCGAGGATTCGTCGTAATT *F TCGTTGTAGAGAACTCCTTGGAACCCAACGAGCAGATGAGTTCGGCGATCTCCCTTAGAACATCGCTATTTCTTTGAAG *F AACGCTGCTTTCCGATTCAATCCTCGCCTCCAGCTCTGAAATTTTGATTTTCGACTCGGTGTCCAGGCGCTGGTACTTT *F CCGTGCAGTTCTCGATTAAGTTGGCTAAAGTTGAGCAGCGTCTACTAAAGCTTCAGCATCTGAGCATCCGGTCCGATCC *F AGGCCCTGATTCGATCACGAGATTAGCAGGATGATCAGGTTGATGAAGAATGTCCATGACACCATGCCTCGGTTTTCCG *F ATGTCCCCCCCGGATCAGTTTTGAAAAGAACACGTTCCCACTCATG *F >SD11641.3prime *F TTTTTTTTTTTTTTTTTTTTGGTTGGGATATGAAAGAAACTTTATTTAAAACTAGTTCATTTCATTAAAACTATTCATT *F TCAGCAATCTGTGATTCTAGTTTCTGTTTAACCATATATAGATAGATATAGATTTGAGTGGAGTTGAGTTTTCGTGGCG *F AACAATCGCGATGATAAATAACTTAAGAACGACAAACTTTAAGCTAAACTATACAAACTTCTTAACGTGGCTTAGATTA *F GACTAACTGTTTAGGAGGCCCAACAAGTGACATAGTTACTTGGAGGTCCTAAACGTTGTAGTTGTGTTTTTCGTTGGTA *F TAGTTTTAGTTGTCGTTGGCTTGTTTGAAGGTTTAGTTGTTGGTTGTTGTTTGTTTGTTTTTGCCGTGGCTCCCGGGAT *F TTTGGTGGTTTTGGTTGGCTTCCTTGTTTGGGAAGGTGTTTTCGTATCGCATTCAATTGCCACTTGATTTTGCTCCTCC *F TCGAATTTCCGTGCAAATATATCCGCAATGGTCGTACAAAATGGATTTTCTTTCGAGGATTCGTCGTAAATTTCGTTGT *F AGGAGAACTCCTTGGAACCCAACGAGCAGATGAGTTCGGCGATCTCCCTTAGAACAACGCTATTTCTTTGAAGAACGCT *F GCTTTCCGATTCAATCCTCGCCTCCAGCTCTGAAATTTTGATTTTCGACTCGGGTGTCCAGGCGCTGGTACTTTCCGTG *F CAGTTCTCGATTAAGTTGGCCTAAGGTGGAGCAGGCGTCTACGTAGAGCTTCCAGCATCTGAGCATCCGCGTCCAGATC *F ACAGGGCTCTGATTCGGGATCAACGGAGATTAGGCAGGATGATCGAGGTCTGACTGAGGATGTCAAATGACTACATGCT *F CCCGAGTTTTTCAAAGGGCTCCCGCCGGATACAGATTTCGATAAGCAGGCAACGTTTCCCATCCCATGGCTGGGACCGG *F TTGGTGGCATCAGAGAATATTGGCTCCGGTCACCCCGACTCCCCCTGGGTCGGATAGAAATCCGCATCTGTATGGCCGG *F AGGTTTTGGAATCCTGGGGA # *U FBrf0188502 *a Shirras *b A. *t 2005.5.31 *T personal communication to FlyBase *u *F Subject: RE: FlyBase query: Orct *F Date: Tue, 31 May 2005 17:34:49 \+0100 *F From: 'Shirras, Alan' *F To: 'Gillian Millburn \(Genetics\)' *F Gillian, *F Sorry about this. Haven't looked at it for some time. Your assessment is *F correct. Y12399 is chimeric and Orct is CG6331. The other Orct gene is *F CG13610 which actually lies upstream of Orct. The confusion was partly due to *F the two genes being almost identical in nucleotide sequence towards the 5' end *F and us relying on Southern blotting at the time. *F Alan *F \-----Original Message----- *F From: Gillian Millburn (Genetics) mailto:gm119@gen.cam.ac.uk *F Sent: 31 May 2005 11:51 *F To: Shirras, Alan *F Cc: gm119@gen.cam.ac.uk *F Subject: FlyBase query: Orct *F Dear Dr. Shirras, *F I am working for FlyBase and I was hoping you could help me with a *F question about the Orct gene, which was described in your paper: *F Taylor et al., 1997, Gene 201(1-2): 69--74 *F A user recently wrote in to FlyBase asking why we had 2 completely *F different cytologies for the Orct gene \- 29D (from your paper and the *F associated accession record Y12399) and 95F (which came from the *F association of Orct with the CG6331 annotation). *F I looked in to this and established: *F a. that the protein sequence of the Orct gene from both Y12399 and *F Y12400 maps to 95F and corresponds to the CG6331 annotation. *F b. however, the Y12399 accession number appears to be chimeric: *F \- bp 1-981 (which include the protein encoded in this sequence *F accession, protein ID CAA73030) maps to 95F8 and corresponds to *F CG6331. *F \- bp 984-2458 map to the 29D region, in an intron of CG17384 (which *F is just next door to the lmg gene). *F presumably this is why you had the cytology of 29D in the Y12399 *F accession ? *F I was wondering whether you agree with my assessment that Orct *F corresponds to CG6331 and that Y12399 is chimeric and whether you have *F any extra information regarding this. I need to correct the FlyBase *F record to alert users to the discrepancy, and was thinking of adding *F the following comment under your Gene paper (and a similar comment *F under the reference for Y12399): *F FlyBase curator comment: in FBrf0099562, the Orct locus is stated *F to be adjacent to the P{PZ}lmg03424 insertion, which is at 29D. *F However, the protein sequence of Orct (Q9VCA2) corresponds to the *F annotation CG6331, which maps to 95F. The discrepancy may be due to *F the sequence of the Orct accession Y12399, which contains *F sequences from two separate genomic regions; part of the Y12399 *F sequence corresponds to CG6331 at 95F, while the other part maps to *F an intron of CG17834 (which is adjacent to lmg) at 29D. *F (FBrf0099562 is the FlyBase ID number for your Gene paper). *F Also, in your personal communication to FlyBase, FBrf0125046, dated *F 2000.1.5, you referred to a second Organic cation transporter gene, *F 'Orct2': *F you stated: *F 'Yes, R3 and 7d are still thought to be Orct cDNAs and the 5' ends map *F upstream of Acer. Both Acer and lmg lie in an intron of the R3 form of *F Orct, lmg being transcribed in the opposite direction. Thanks to the *F genome sequencing projects, we have found that the structure of the 7d *F transcript shown in the paper is wrong \- what we have shown as the *F downstream exon of 7d is in fact another organic cation transporter *F gene.' *F This other 'organic cation transporter gene' is currently called *F 'Orct2' in FlyBase. I was wondering if you know which CG gene *F prediction 'Orct2' corresponds to \- at the moment we do not have it *F mapped to the genome, and it would be great to get it tied down to a *F particular annotation. *F thanks, *F Gillian *F \-------------------------------------------------------------- *F Gillian Millburn. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: gm119@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://fbserver.gen.cam.ac.uk/ *F \-------------------------------------------------------------- # *U FBrf0188504 *a Xie *b H. *c K. *d Golic *t 2005.6.1 *T personal communication to FlyBase *u Sir22A-7-11, Sirt25B-2-35 and Sirt4white+1. *F Date: Wed, 01 Jun 2005 16:01:07 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Sir22A-7-11, Sirt25B-2-35 and Sirt4white+1 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Heng Xie and Kent Golic, University of Utah. *F Sir22A-7-11 is a deletion of the Sir2 coding region produced by ends-in *F gene targeting followed by I-CreI-induced deletion. PCR primers sir2-C1 *F (5'-CCAAATGGGTGCGAAGCTGACG-3') and sir2-C2 (5'-GGCCCTCGGCTACGATTTCGCAG-3') *F give a 0.3kb band for the mutant allele and a 3kb band for the wild type *F allele. The donor template and technique used to generate this line is *F described in Xie and Golic, 2004, Genetics 168(3): 1477--1489 *F (FBrf0180287). *F Sirt25B-2-35 is a deletion of Sirt2 coding region produced by ends-in *F gene targeting followed by I-CreI-induced deletion. PCR primers CG5085-C1 *F (5'-GCCCCAGGCTAGTCTAAATAG-3') and CG5085-C2 (5'-GAAAGAAAGCTCGCGCTATTAG-3') *F give a 0.2kb band for the mutant allele and a 1.4kb band for the wild type *F allele. The donor template and technique used to generate this line is *F described in Xie and Golic, 2004, Genetics 168(3): 1477--1489 *F (FBrf0180287). *F Sirt4white+1 is a deletion of Sirt4 coding region produced by ends-out *F targeting. The miniwhite marker was not removed by Cre-mediated recombination. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188505 *a Gong *b W. *c K. *d Golic *t 2005.6.2 *T personal communication to FlyBase *u Gong & Golic Hsp70 deletions. *F Date: Thu, 02 Jun 2005 10:25:51 \-0500 *F To: gm119@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Gong & Golic Hsp70 deletions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Wei Gong and Kent Golic, University of Utah (11/04). *F Hsp70Ba304 is a deletion of the Hsp70Ba gene produced by ends-out gene *F targeting. The miniwhite marker has not been removed by Cre-mediated *F recombination. *F Df(3R)Hsp70A is a deletion of the Hsp70 genes at 87A (Hsp70Aa and Hsp70Ab) *F produced by ends-out gene targeting. The miniwhite marker has not been *F removed by Cre-mediated recombination. *F Df(3R)Hsp70B is a deletion of the Hsp70 genes at 87C (Hsp70Ba, Hsp70Bb, *F Hsp70Bbb and Hsp70Bc) produced by ends-out gene targeting. The miniwhite *F marker has not been removed by Cre-mediated recombination. *F The donor templates and technique used to generate these lines are *F described in Gong and Golic, 2004, Genetics 168(3): 1467-1476 *F (FBrf0180286). Hsp70Ba304 is one instance of the targeted deletion class *F described as Hsp70Ba- in this paper. Likewise, Df(3R)Hsp70A is a *F representative Hsp70A- event and Df(3R)Hsp70B is a representative *F Hsp70B- event. *F The paper describes recombinant chromosomes combining a Hsp70A- deletion *F and either a Hsp70Ba- deletion or a Hsp70B- deletion. Two of these *F recombinant chromosomes donated to the Stock Center are *F Df(3R)Hsp70A, Hsp70Ba304 *F and *F Df(3R)Hsp70A, Df(3R)Hsp70B *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188518 *a Brown *b N. *t 2005.6.15 *T personal communication to FlyBase *u FlyBase error report for CG31794 on Wed Jun 15 04:51:12 2005. *F Date: Wed, 15 Jun 2005 04:51:12 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: n.brown@gurdon.cam.ac.uk *F Subject: FlyBase error report for CG31794 on Wed Jun 15 04:51:12 2005 *F Error report from Nick Brown (n.brown@gurdon.cam.ac.uk) *F Gene or accession: CG31794 *F Gene annotation error *F Gene CG31794 has incorrect exon/intron structure. *F Comments: According to my reckoning there are a couple of things wrong with *F the annotation report *F 1. you are missing the second start, as indicated by the ESTS RE08621, *F RE18088. This has a new protein start in the second exon, which is conserved *F in other Drosophila species and anopheles *F 2. There is no evidence yet for transcripts like RD and RE. The one cDNA with *F this start that has been sequenced is the published AB056465, which has a *F different 3' exon. *F 3. The starts of RD and RE are in fact the same the same; one can find a *F little bit of the first exon of RD in all the RE cDNAs (too short to come up *F in a blast). *F 4. RF and RC seem the same *F Hope this is helpful, Nick # *U FBrf0188523 *a Hacker *b U. *t 2005.4.28 *T personal communication to FlyBase *u *F Date: Thu, 28 Apr 2005 09:22:44 \+0200 *F From: Udo Haecker *F Subject: Fwd: (Fwd) FlyBase query: gm13666 *F To: gm119@gen.cam.ac.uk *F >Dear Gillian, *F > *F Christian Lehner forwarded your email to me. The *F answer to your question is yes. The Mps11 *F allele is the same thing as line PL00781. *F Best Regards *F Udo *F ... *F > *F >Dear Dr. Lehner, *F > *F >I am curating your paper for FlyBase: *F > *F >Fischer et al., 2004, Curr. Biol. 14(22): 2019--2024 *F >'The mitotic arrest in response to hypoxia and of polar bodies during *F >early embryogenesis requires Drosophila Mps1.' *F > *F >and I have a couple of questions about some of the lines you used. *F > *F >1. Mps11 ( = superscript). *F > *F >In the supplementary material, you say that this line is corresponds to *F >Mps1Bac{3xP3-EYFP, p-Gal4D-K10}715 and is described in: *F > *F >Hacker et al., 2003, Proc. Natl. Acad. Sci. USA 100(13): 7720--7725 *F > *F >I had a look at Supplementary Table 3 for this PNAS paper, which is *F >available at: *F > *F >http://www.pnas.org/cgi/content/full/1230526100/DC1/3 *F > *F >and there is one line in that Table that mentions CG7643 (which is *F >Mps1) \- that line is PL00781. Is your Mps11 allele the same thing as *F >line PL00781 ? (I am trying to avoid duplication in the database). *F > *F > *F >thanks, *F > *F >Gillian *F > *F >-------------------------------------------------------------- *F >Gillian Millburn. *F > *F >FlyBase (Cambridge), *F >Department of Genetics, *F >University of Cambridge, *F >Downing Street, email: gm119@gen.cam.ac.uk *F >Cambridge, CB2 3EH, Ph : 01223-333963 *F >UK. FAX: 01223-333992 *F > *F >FlyBase: http://fbserver.gen.cam.ac.uk/ *F >-------------------------------------------------------------- *F >--- Ende der weitergeleiteten Nachricht / End of forwarded message \--- *F >-- *F >Prof. Christian Lehner *F >Lehrstuhl für Genetik *F >Universität Bayreuth *F >95440 Bayreuth *F > *F >e-mail: chle@uni-bayreuth.de *F >tel: \+49 921 55 2701 *F > \+49 921 552702 office *F >fax: \+49 921 55 2710 *F \-- *F \-------------------------------- *F Udo Haecker *F Department of Experimental Medical Science *F Division of Developmental Biology *F Klinikgatan 26, BMC B13 *F Lund University *F 22184 Lund, Sweden *F phone: \+46 46 2220864 *F Fax: \+46 46 2220899 *F Homepage: http://www.cmb.lu.se/devbiol/uhdevbiol *F http://www.stemcellcenter.se # *U FBrf0188528 *a Collins *b R.T. *t 2005.7.7 *T personal communication to FlyBase *u *F Date: Thu, 7 Jul 2005 14:44:39 \+0100 *F To: gm119@gen.cam.ac.uk *F From: Russell Collins *F Subject: Genetics 2005 170:173-185 *F Hello Gillian, *F We recently published a paper (Collins and Colen, Genetics 2005 *F 170:173-185) describing a screen to identify novel components of the *F Hh pathway. Some specific information, such as the map location for *F some of the groups and which alleles were enhancers or suppressors, *F was excluded for the paper in the interest of brevity. This *F information is presented in the attached table. *F Best, *F Russ Collins *F Date: Sun, 10 Jul 2005 12:53:19 \+0100 *F To: gm119@gen.cam.ac.uk *F From: Russell Collins *F Subject: table (second try) *F Hi Gillian, *F Here's an updated version of the table. I added group G and its map *F position, and removed the Group G members (i.e. 1440, 2050, 2291) *F from the single hits. *F I also noticed that mapping information for Group B-left says that *F position was determined by male recombination, but did not include *F the P insertions that it maps between. I added that info into the *F updated table as well. *F ... *F Russ *F \-- *F FlyBase curator comment: the changes to the original table *F (Collins.2005.7.7-1.doc, deposited at Indiana) in the updated version *F are: *F 1. the following 3 alleles, previously listed as single hits, have been *F removed: *F 1440 E *F 2050 E *F 2291 E *F and have been added as a single complementation group (Group G): *F G 1440 E E(smoDN)G1440 64E5;64F5 *F 2050 E E(smoDN)G2050 fails to complement: *F 2291 E E(smoDN)G2291 Df(3L)Exel6107 *F 2. the mapping information for Group B-left has been changed from *F 'mapped by male specific recombination' to 'mapped by male specific *F recombination between: EP(3)0794 and EP(3)0917'. *F \-- *F File deposited: Collins.2005.7.7-1.doc ; File date: 2005.7.7 ; File size: *F 120320 ; File format: doc # *U FBrf0188544 *a Hutter *b P. *t 2005.7.25 *T personal communication to FlyBase *u Comments on the RAB9D gene. *F From: Hutter Pierre *F To: ''Gillian Millburn (Genetics)'' *F Subject: RE: comments on the RAB9D gene *F Date: Mon, 25 Jul 2005 18:29:24 \+0200 *F Dear Gillian, *F I clarified the site of insertion of the PBac{WH}Rab9Df06390 transposon *F (Thibault et al. 2004)in Rab9D by genomic sequencing, which placed it at *F the TTAA target site at nucleotides 23-26 (from the A of the start codon of *F Rab9D). In D. melanogaster Hmr1 (hybrid rescuing mutant) flies, the Rab9D *F gene contains five nucleotide changes, which lead to four amino acid *F substitutions. Interestingly, all four replacements correspond to amino *F acids present at the same sites in proteins encoded by some of the five *F paralogs *F of Rab9D. Moreover, the Rab9D gene has considerably diverged between D. *F melanogaster *F and its close relative D. mauritiana, whose Rab9D sequence was reported last *F year under GenBank accession number AY830113. I also observed that Rab9D *F appears to have evolved under particularly strong positive Darwinian *F selection, as shown by pairwise comparisons of Ka/Ks ratios, which are *F higher than 2 between Rab9D and its paralogs CG32670 or CG2885. It should *F also be pointed out that no ortholog of Rab9D can currently be identified in *F genomes from other arthropods, *F suggesting that Rab9D and its three closest paralogs are unique to D. *F melanogaster. *F Thank you very much for your assistance. *F Pierre Hutter, PhD, FAMH *F Chef de la Division de Génétique Médicale *F Institut Central des Hôpitaux Valaisans *F Av.Grand-Champsec 86 *F 1951 Sion. Switzerland *F Phone: 027 603 4850 *F Fax: 027 603 4995 *F e-mail: pierre.hutter@ichv.ch # *U FBrf0188545 *a Scholz *b H. *t 2005.7.25 *T personal communication to FlyBase *u *F Date: Mon, 25 Jul 2005 07:45:02 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 060 annotation of transcript *F I have sequenced the clone GH14331 belonging to the CG4411- hangover gene. *F I further used this EST to generate an uas construct that was used to rescue *F the ethanol phenotype of the mutant hangAE10. The mutant will be published in *F near future in Nature. *F I would like to fix the sequence for the GH14331 clone. The annotated exon *F and intron structure of the gene changed a little bit. *F Please find the sequnence at the end of this mail. For further questions, *F please email me. *F Thank you very much in advance. *F Best Wishes *F Henrike Scholz *F >GH14331_Drosophila_hangover sequence *F GAATTCGGCACGAgGTTTACAGTCGCAGATACGCCACGGCAATTCGCAGT *F CCCAGTGTAGCGGTCATTTTTTTTCCAGTG *F CGACTCCTCCAAACAAAAGCGAAGTGGAAAAATAGGCGAAGATAAAGGAA *F ACCCAAGAGCGAAACTCCAAATTGCGAATC *F AGCAAAGGATCAATGGCAACGAAATAAGTAAAAGAACCAGTGCGAACAAT *F GTGCGAAAAGTGGGAAACGCGAGCGGCGTG *F GTCGTTTCCGTCGGAGAGCGGATAATATAGCACATAGTAGTATATTGTAG *F TAGTGGATGTGGATGGTGTGGAACGATTGC *F GAACGGACGCAGACCGAAACAAAAGTGAAAACGTGCAAAAAACCAAACCC *F AAAAACAAATTCAACCGAGAAGTAGAAGCA *F AGCGGCAAAGAAGAGAGCGAGAGGGCGACCAATACAAAAGCGACAAAATG *F TGCGACGCTGCGGCGGCAACAGCGACAACA *F ACAACAACAGCAGCAGTAGCAGCAGCCGTCGCAACAACAACAGCATCGGT *F AGCATTGGAGGCCACAGCGACACAGCCTGG *F AACGACAACGACGACGGTAGCCACTGCGTCAGCGGGAACCACATCCCCTG *F AAGCGGCCATTCCAACAGCAGCAACAGCAA *F CATCGGCGAGAAACAGCAACAGCGAACGGTCGGCGCGACAAAACTGCTGT *F CGCCTGTGCATCGCCCCACAAACCGAATGC *F ATCTCGATCATCAATAGCTATGCGGCCGACAAGGAGCCGCTGTCCACCAA *F GATCCACAACTGCGTCGGCATCAAGGTTAC *F ACCGCAGGATCGGCTGTCGCAGCAAATTTGCCACGCCTGCATCAGTTACC *F TGAACTCATGGCAGAGCTTCAAGAACCGGT *F GCTTCAGCTCGCAAGCGAAGCAGCGCCAGTGGCTGGATACCAACAAGAGC *F AAGCTCCTCAACTACCTGGACCTGAACAGT *F GCCGAGAATGGGGGAGGAGGCTTCTTCGATCAGCATCTGCATCAACAACA *F GCAACACCACCAGCACTTGGAGAATGAGCT *F CGAAGCGGAGAAGGAGAAGGCGACGCCAACGGCCGCATCGACAGCCGCCA *F ACATATTGGACGGCATACACTCGCTGAAGA *F AACGCAAATCCCTAACAGTCTATCCACTGCCTGCGATGCCCATCAAAGAT *F GAGCCGATTGATACGGATGACGACTATCAG *F ATGAAGTCCATAGACGAGTCCGATGACATGGTCGATCCCACAATGTTCCT *F AGAGCGCTCCGAGCACGAGGGTGATGTCCC *F GCTGACGGCCTCGGATTACGACTACACTGCGCAGCATGGCGTGAATGCTT *F CGTCCGTGGCTGCCTCGCTGCCGCCGAATG *F CGGTGGCCAATGTGGCAGCCGCCGGGGACTCCAAGGTGGCCAGCTGCAGG *F GCCTGCAGCCTGCAGTTCTCTACCCGGGCC *F AACGCCCGTCGCCACGAAAGGAATCTACACCCAAACCTGTTCCAGTTGTC *F CACAGACTCCCCCCACAACACACCCATCAC *F AAAGCCAACGCCCGCTTTGGCTGCCGCCTTGGAAATGCAACGGGCAGCGG *F CTGCGGCGGCCACCGCTGAGGCAAATCGGG *F CAGCGGGCGCCGCTGGCGGGAATATCTCCACGCAAAAGTACCGCCAGGTG *F GTGATGAACGCGTTCATTAAGTGCGAGGGC *F GGCGGCTACGACTACGATAATCCCGAACAGTACCGACCATTGCTCACCCG *F CGACAAAGTGGAGTTCATTGAGCAGAACGA *F TGAGTTCCTCGAGCAATACCAGACGATGACCTGCCGATGTTGCAACAAAT *F ACTTCAGCACGTACAAGAACTTCATGGCGC *F ACGTTCGCAAGAAGTATCCGCAGCTGCCGCGCAACCTATGCTTCAATTGC *F CTCAAGATGAACGACTCCAAGGCGCTGTTC *F ATCTCGCATCTGAAGAAGCGGAATTGCATAAATCTCTTTAGAGTCTTGAA *F TGCGTTGCGTGGGAAAACAACGACAGTGGT *F TGTGCCCATTGCAGATGATGTGGCAGACGATGGAGCAACAGGATCTATTC *F CAGTTGCCGATGCCGGAGCAGGAGTGGTGG *F CCATGAATAGTCCCACAGTGACAGCCAGCGGAGAAGTTGTTACACCCGGC *F GGCGGCTCCGAGCGCCCAGAGAAACTGCGC *F GCCAAGGAACTTCTGGTCAACAAACTGTACGAGTGCAAGCTCTGTCCCAA *F GGGATTCCGCACTAAGCACGAGTTCCGAAC *F GCATGTCTACGACAAGCACGCAGATGTCCAACGCAAGGATAACAACTCGA *F TACAGTGCAGCTTTTGCGGACTGGACTTTG *F CCGATCCCGTTGACAGACGGCGTCACTACAACAACATGGACTGCATTGTC *F CGGCTGCGCTGCATGACGTGCGATGCCAAG *F CTGGAAACGCACCAGCGATTCCTCGATCATGTCTACCAGGATCACTTGGG *F CGGTGTGGGTGGTGGCGCGGTCAGCGACAA *F CGCCTCCACCACTGGCAGCGGCATGGCCAGGAGCAACAGCATGGAACACT *F CGCCGGGCAAAAGGAGCCTGCTCGGCGCCT *F TAGGCGTTGGTTCCTCGGCGGAGGAGTCGCGAAGCAGCAGTGCGGCTCCG *F CCGCTGACCTCTACACCAAAACTGGCGGGC *F GGTAATCAGGTGGGTGGCGGTGGATCGACCAGCGCTTCTGCCGCCGCAGC *F CGCTCAGAGCTCGGCGAATCGGGATGCATC *F CGCACCCAAATCCCAGTACTTCTCCCGTATGCCGCAGGTTTGTCCCATTT *F GCGGCCAGCAGTACAACAACTACAACAATG *F TGCTGCGCCACATGGAATCGAAGCATCCGAACAAACTGCCAGAGACATAC *F AAGTGCGTGCGCTGCGGACTGGGCTATCCC *F CGGATCTCCTACCTACGGGAGCACATGATCAATGTGCACGGAGTGGACAA *F GAACCGTCACTCTGGCGGCTTCGAATACAT *F CGTGAATGCGGATGCCGTGAAGTTGGCGGACGGAAGCACGCCGAACGTCT *F ACACGGGTCGCTACGATTACGTGATGAAGG *F ACCTGATGTCGATAACAAATGATGATGAAGAGGAGGAGCCTGGCAGCGTG *F GCCAAGAAGATGCGCCTGGATGACAGTAGC *F AACAACAGCAGCCTGGTGGGCGTGGCTAGCCAGCAGAAGGAGTGCCCCAT *F CTGCAATGCGGTGTTCAGCAACAACATTGG *F CCTGTCCAATCATATGCGTTCCCACTATACGGCCTCGAATGCAGTGAATG *F CAGCCTTGGCGGCTGCCAATCGAATGACAC *F CCAAATCGCTAACGATAACCGCAACGCCAGCAACGGATTCGGAGTTGGGA *F GTTGGAGGAACAATGTCCGAGTCGGCTCCA *F GCAACGCCTGCCAATGTGCCACCGGCAATGGCTAACCAAACGCCCCAGGA *F GCAGGCAGTTTTTCGCCGGAGCCTTGACCA *F GGCAGCCGATCGCCGCTTCCGGCGAATGCGATGCCGCATCTGCCAGCGTC *F GCTTCAGTTCGAAGAAGTCCTATCGCTACC *F ACATGCTCACCGATCATCAGGTGCAGAATGTGCAGTTCATCAAATGCAAG *F CTGTGCAACGCAGAGTTTGCCTACGAGAAG *F GGCCTGAAGGTGCATCTGTTTAAGGTGCACGGAAAGGCCATCAAGGATGA *F AATGATTATCAAGCAGTTCGAGTGTGACGT *F TTGCTCGATTGTCTATAGTTCAGAGTCGGAGCTGCAGCAGCACAAACGCA *F GCGTTCACAAGCTGACATCCGCCTCCGCTT *F CCACTTCGGCGTCCACGTCCTCCAAGATTGACGACGACTCTCTAATGGAT *F GATGGCAAGCCGACATCATCGGATCTAGCT *F GATCTCTCTACCCTCGCAGCGGGTGGATCAACTGCATCTGCTCCACTGTA *F CTGGTACCAGTGCAAGTACTGTCCATCAAA *F CTTTAACACCAACAAGAAGCTGGCCATCCACATCAACTCGCATGACGAGT *F TTGACTCGAACGATTATTCCTGCAAAGATT *F GCGGAAATGTCTACAGCGGACGCAAGAGTCTATGGGTTCATCGCTATAAG *F AAGCATCCGCAAGTGCCCAACCCAGCTGAG *F TGCTCGCTGTGCCGCAAGGTCTTTTTCGACCGCCAGATGCACGACAACCA *F CACACCCACCTGCAACCGCAAGCCGATCAC *F CTCGACAGGTGCCCACCAGCAGCAGGATGGACAACTGCATTCGCACCACA *F CGGCCAAAAGAACAATTTTCCGGCATAAGA *F CCGGCGATGACGATGACGAGGAGGATGACGATGAGCAGCAGCAACTGGAG *F GAGAGGGCAAATAGCGATGGCAATGGCACC *F ACTGTGGGAGTGGCGTCGGGCAGTACTGCAGCTGCGGGCACGTCACTAAA *F GATCCGCATTCCGGAGGTGGCGTGCACTAT *F TTGCGGTGCTCGCTTCACCGACCAGGAGCACTTTAGCAAGCACATCCAGA *F AGCACGAGCAAGAGCTGTACGTGGACAATC *F CGCTGGCGGCGATGTTCGATGATGGGCCGGCGGATGCCGGTCAGTTCCAG *F GTGGAGCGGCAGAACGAGAACGGGGAATAC *F GCGTGCGATTTGTGCGCCAAGACGTTCCCCCAGGTGATCGCACTCAAGGT *F GCATCGCAAGTGGCATTTCAGAGGTGATAG *F CAAGCAGAACCCCATCGACGGCGAAGCGACACAGTTGACCAACAACAATC *F ACACAACCAACAACAACAACAACAACTCGA *F TGCACCTCCGCGAGCTGCATGCGGTGGGCCTGATGCCCAACCAGCAGCAG *F CAGAGCCTCAACAACTCGTGCAACAGCAGC *F ATGAACCACAACAACAACAGCAGCAGCAACCGCAGCAAGTCGATGAAGCG *F GAAACGTGAGCTGAAATGCGAATACTGCGC *F CTCCACCTTCATCAGCAATAACAATCTGCGTCGCCACATGTACGAGCTGC *F ACAAACACGAGGTAAGCAATCTGCCCGAGC *F CTCCGGTGATTGTGGTGGACGATCACCTCACCTGCCGTCGCTGTCAGTTG *F AAGTTCGACACAAAGGAGCTGTGGATCGAG *F CACAAGCTGGCCGATGCAAAGGTGGTGCGACCTTTCTGCCCCTTCCAGTG *F GGGCTGTGATCTGTGCGGCGAGTATTTGTC *F GCGCAAGGAGAAGCTAATGAATCACATTAACAACCATTTGAAGGAGGAGG *F TCATAGTGCCAGTAGCCACTAAGGCGGCCA *F TAGAGAGAACAGCGGCCATGGAATCAGCGGCAGCAGATGCGAATGCAGCG *F GCGACACTATCAGCATTGGGCGAAGGAGCC *F GAAACTGAAGATCAGTTTGCAGAGAAGGTTGAGGCTGCAGGGGCAACAAC *F AACAGATAAGTTGACGAATCCCGACGAGGA *F GGATAGCGATGATTTGGATGAGGATAGCTCGGGCGACGACGATGATAGTT *F CGGGGACGGGAGATGATGACGATGACGACG *F ACAGCGACGATGATGAGGATGGCGAGGGTGAAGATGAGGACGAGGAGGGA *F GATGGTGGCGAAGGCGAGGACGAAGAGGGT *F GTCCAGCCACCCGCTCAACTTTTGCCGCAGCAGCAACACAAAACGGATCT *F TAATCTTAACCAGGACGACGATGATCTCGT *F CGAGGAGGTCATCAGCTCCGATGACGACGAGGACGATGATGGAGAGGTGG *F AAAGCGACGATGACGACGAAGATGATGATG *F ATGAGGAGGACGATGTGGAGGAGCCGGAGCCAGTTGGATTGACGGTGCGA *F CCGCTAATGAATGGCAAATCAAAGATGCCG *F CCGCTAATAGTGGCCAGTTCGGATGATGAAGACGATGGTGTGATGCCCAT *F AGAGGACATTATCGAAGAGGAGTTCGATGA *F GGATGCCGATCCAGATCCGGAGGATGCCATTGAGGAGGTAGACGAAGATG *F ATTTAGATGAGGGGGAAGTGGAGGACGAGC *F CGAATGTGGTGTCGACGGCCTCCTTCTCGGAGAGCGAGTCCAGCACAACG *F ACCACGTCCAATTCGCATTCGCATTCGCAT *F TCAACGGGAGCTTCTCCAGAGCCACATCAAAAGCCATATCCTCAATGGGC *F CAAAGCTCTCGACAGTGTCAGCAGCAGCAG *F CAGCAGCAGCAGCCGCCGCCACAGCAAGCAGCAAGGCAACAGCATTACTA *F ACGGCAGCAAAGGCGAAGCCTGACTCCAAG *F TCAGCGGTGCTGGCCAACAATAATAACAGCAAGACAAGCAGTAAGACTGT *F GGCAGCAGGTGCGACAAATTGAGTGATCAT *F ACTCCAACTACAACTCCAACTACCACTACCACTACCAACCACAACTACCA *F ACCACACCTCATCATCTCAATGCAAAATCA *F CTTGAAATGTACAAAGCCAATCCAATCAGACACCGCCATCATCCATCTCA *F TCATACTCGTAATCATCATTCGAACAATGA *F ATACATCAGCAGCCACATAAACATCAGCAACGCATTCCATCAACAAATCA *F GCCACGTTTATGTATAGCACGAGATGCGTG *F TGTGTGCGTGTGTGTTTATATAACAAAACTTTTTTTTCTTGCTTCTAGTC *F TTAAGAACTTAAATTACATATATGTACATC *F ATATATAAGACTTTTTTTTTAAATGTTAACCTAGTATGTACCGCGCACAC *F ACCCACACACAAAGCAAATTATGGGAACCA *F TAGACATCCCTCTACTGCTAAGTTTCGCTATTAAAACTATATATATATAT *F ATATAAATATATATACATACATTGATATAG *F TAGGATTAAGCATATAGAAAAGTACGTCTTAATATGTTACACATCAATTA *F ACAAGCAAAAAGAAAAAGTTTACGACGCAC *F CAAAGCAAGCGACAGAAAACGCAAGGGAAACCGCTCAGTTAGAGTTAGTA *F AAAACTATCAGGATTTTCAAGTATTTCACA *F CAACACCATGACACTCCCAAAAAAAAAAAAAAAAAAAAACTCGAGTTA *F >Hangover protein *F MCDAAAATATTTTTAAVAAAVATTTASVALEATATQPGTTTTTVATASAGTTSPEAAIPTAATATSARNSNSERS *F ARQNC *F CRLCIAPQTECISIINSYAADKEPLSTKIHNCVGIKVTPQDRLSQQICHACISYLNSWQSFKNRCFSSQAKQRQW *F LDTNK *F SKLLNYLDLNSAENGGGGFFDQHLHQQQQHHQHLENELEAEKEKATPTAASTAANILDGIHSLKKRKSLTVYPLP *F AMPIK *F DEPIDTDDDYQMKSIDESDDMVDPTMFLERSEHEGDVPLTASDYDYTAQHGVNASSVAASLPPNAVANVAAAGDS *F KVASC *F RACSLQFSTRANARRHERNLHPNLFQLSTDSPHNTPITKPTPALAAALEMQRAAAAAATAEANRAAGAAGGNIST *F QKYRQ *F VVMNAFIKCEGGGYDYDNPEQYRPLLTRDKVEFIEQNDEFLEQYQTMTCRCCNKYFSTYKNFMAHVRKKYPQLPR *F NLCFN *F CLKMNDSKALFISHLKKRNCINLFRVLNALRGKTTTVVVPIADDVADDGATGSIPVADAGAGVVAMNSPTVTASG *F EVVTP *F GGGSERPEKLRAKELLVNKLYECKLCPKGFRTKHEFRTHVYDKHADVQRKDNNSIQCSFCGLDFADPVDRRRHYN *F NMDCI *F VRLRCMTCDAKLETHQRFLDHVYQDHLGGVGGGAVSDNASTTGSGMARSNSMEHSPGKRSLLGALGVGSSAEESR *F SSSAA *F PPLTSTPKLAGGNQVGGGGSTSASAAAAAQSSANRDASAPKSQYFSRMPQVCPICGQQYNNYNNVLRHMESKHPN *F KLPET *F YKCVRCGLGYPRISYLREHMINVHGVDKNRHSGGFEYIVNADAVKLADGSTPNVYTGRYDYVMKDLMSITNDDEE *F EEPGS *F VAKKMRLDDSSNNSSLVGVASQQKECPICNAVFSNNIGLSNHMRSHYTASNAVNAALAAANRMTPKSLTITATPA *F TDSEL *F GVGGTMSESAPATPANVPPAMANQTPQEQAVFRRSLDQAADRRFRRMRCRICQRRFSSKKSYRYHMLTDHQVQNV *F QFIKC *F KLCNAEFAYEKGLKVHLFKVHGKAIKDEMIIKQFECDVCSIVYSSESELQQHKRSVHKLTSASASTSASTSSKID *F DDSLM *F DDGKPTSSDLADLSTLAAGGSTASAPLYWYQCKYCPSNFNTNKKLAIHINSHDEFDSNDYSCKDCGNVYSGRKSL *F WVHRY *F KKHPQVPNPAECSLCRKVFFDRQMHDNHTPTCNRKPITSTGAHQQQDGQLHSHHTAKRTIFRHKTGDDDDEEDDD *F EQQQL *F EERANSDGNGTTVGVASGSTAAAGTSLKIRIPEVACTICGARFTDQEHFSKHIQKHEQELYVDNPLAAMFDDGPA *F DAGQF *F QVERQNENGEYACDLCAKTFPQVIALKVHRKWHFRGDSKQNPIDGEATQLTNNNHTTNNNNNNSMHLRELHAVGL *F MPNQQ *F QQSLNNSCNSSMNHNNNSSSNRSKSMKRKRELKCEYCASTFISNNNLRRHMYELHKHEVSNLPEPPVIVVDDHLT *F CRRCQ *F LKFDTKELWIEHKLADAKVVRPFCPFQWGCDLCGEYLSRKEKLMNHINNHLKEEVIVPVATKAAIERTAAMESAA *F ADANA *F AATLSALGEGAETEDQFAEKVEAAGATTTDKLTNPDEEDSDDLDEDSSGDDDDSSGTGDDDDDDDSDDDEDGEGE *F DEDEE *F GDGGEGEDEEGVQPPAQLLPQQQHKTDLNLNQDDDDLVEEVISSDDDEDDDGEVESDDDDEDDDDEEDDVEEPEP *F VGLTV *F RPLMNGKSKMPPLIVASSDDEDDGVMPIEDIIEEEFDEDADPDPEDAIEEVDEDDLDEGEVEDEPNVVSTASFSE *F SESST *F TTTSNSHSHSHSTGASPEPHQKPYPQWAKALDSVSSSSSSSSRRHSKQQGNSITNGSKGEA *F Molecular Weight 208176.55 Daltons *F 1901 Amino Acids *F 193 Strongly Basic(+) Amino Acids (K,R) *F 287 Strongly Acidic (-) Amino Acids (D,E) *F 519 Hydrophobic Amino Acids (A,I,L,F,W,V) *F 599 Polar Amino Acids (N,C,Q,S,T,Y) *F realname: Henrike Scholz *F reply-to: henrike.scholz@biozentrum.uni-wuerzburg.de *F source: FB Help Mail: *F usersubject: annotation of transcript *F Sent from computer 132.187.25.56 # *U FBrf0188546 *a O'Farrell *b P. *t 2005.8.1 *T personal communication to FlyBase *u *F Date: Mon, 1 Aug 2005 20:44:37 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 077 Gene data (problem or question) *F comments: Flybase includes entries for a gene called Spred and a gene *F CG10155. These are the same gene. This can be seen by looking up the *F sequences for these genes on the NCBI Protein data base using either the CG \# *F or using AE33 (an allele of Spred). Both give the same sequence, but there is *F no cross referencing between either of NCBI entries or the Flybase entries. *F realname: Patrick O'Farrell *F reply-to: ofarrell@cgl.ucsf.edu *F source: FB Help Mail: # *U FBrf0188547 *a Murphy *b T. *t 2005.7.29 *T personal communication to FlyBase *u FlyBase error report for CG14617 on Fri Jul 29 13:52:55 2005. *F Date: Fri, 29 Jul 2005 13:52:55 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: tmurphy@ciwemb.edu *F Subject: FlyBase error report for CG14617 on Fri Jul 29 13:52:55 2005 *F Error report from Terence Murphy (tmurphy@ciwemb.edu) *F Gene or accession: CG14617 *F cDNA or EST error *F Comments: I have recently assembled full-length sequences of 5 cDNAs *F corresponding to CG14617, and they reveal a more complicated intron-exon *F structure than previously thought. FASTA formatted sequences for the 5 cDNAs *F are below. These sequences have also been submitted to GenBank. *F >GH03511 *F CCGTTTGAAAATAAATGTTTTCCCCAGCATTAAAGCAGCATTTCTCATTGGCTCTTGATGGTAGCTGCGATCCACGGTAG *F AAACATTGAATACAAAACTCTCAACGATTCGGGATATCATTTGATTGATGTAATGAACTCTTCAGAGGTTTCCTAATTTT *F TGGCGGGAAGTCGAGGGCTCCCTAGTGCCATCGTCATCTCTAATGGCAACTAAAACATAGCAGACATTTAATGAACTTTC *F ACAAAACACCTAGATTCAGCTGACCTCTGAGAAGGCTTCCTGACATTTCGACGTCCCAACAAAAATTCCCAGTAGCAAAA *F CACCAATGGATGCGACGTGGGCAATGGAGCAGCTGCTGTCTGCAGTTGAGATGGGAGGTAGCCACAGCGAGGCCACCACA *F CCAACGGCCACGACCACCATATCGATCTGGAGCGCCCCCTCCGAGGGCAGTGGCTCTAGTTCCATGGACCCTGTCTCCTT *F TCGGTTCCACTTGGACGGGCAGCCTATACTGCCACCGCTGATGACGAGCGCCAAGAAGCGCGAGGTGCAGCTGGCCCGCC *F AGATGGCAGAAGCGCTGGAGAAGCGGTACCGCCTGGCCAGACACTCGGCGGGATCCGACATGGCCAGTAGGAGAAGTGTG *F TCACAGCTGCAGCAGACAGAAACGTTAATCTATGACAGCACACGGGGCCAGGCTCGTCCTCAGACTCTGGTCTTTGGCAA *F ACAGTTGCCCACCATACAGGTGGATCCTCCCACGCCTCAACCATTTGTGAAATCAATAGAAAACACTTCCCCAAGGCGGC *F ATAATCGCATTACCGATCGTATCCTGCAGTTCGAGCAGTCGGGACTGGGTAAGCTGCTGCCCAAAGTTCCAGATAAGTGT *F ATCCTGCGCACAAGTCCTGCGGTAGCCGAAGACCAGCAGGATCAGGATACAAAGGACACAACGGCAAGGGAGCTCGGCAG *F TCCACTGGCAGAACCAGCTAATTTTCAGCGTTCGAGAAGTTTCACACTAGAGGAGCCCTCCCAGGTTCTCGTGGAGCACA *F TGGAGAGGGAGGCCCTGGCTGTGAAACCCAGCCAGAGTTTGGCCAACTTCGAGAAGCAGACCATCGAGTCTCAGGCCAAG *F CGGGTGAACCGTAGTGCAAGGAGTATTAGCAGCAAAATTAGCCACAACAGCTACAATAGCAACAGTAGCGCCGCAACCAA *F GAGCACCATCGCCACTAAAACCACAACCGCCGGCAGCAGCTGTTCTAGGCGGGATCGCGAGGTGGAGCGTATAATAGAAC *F GGGCGCTGGACGAGCATGGCGCTTTGGAAGCCAGCCGCAAGGCAGGAGTGCGCAACTATCTGCGTGGCCATCGGGAGCGA *F ATGAATCAATTGGTCATTCACCAGGAGAAGGAACGTCGTCGCATGCAGGCCGAGTTTGATCATCAGCAGCGTCAGCTCAT *F AGAGGAACTGTGTGCCGAAATTGATGTTTCCTCGGCCACCGAAAATCCTGACAGTCTGATATCGCAAAGCACCAGTACGG *F GAGACCTGCAGCGTTTTTCCCCCTCGCCTACGTTACCCTCCTTTTCAGAGGACATAGAAGACTTTCCGACGATGGAAGAC *F AAGTGCCAAATTGCGGGGCCTTCTTCCGCTCGCAAGAGGCTGTTTAGTCCAAAACCATCAAAGGACTCGGAACCGCAGCT *F GCTCAGTGCCCCCAGCACACCGCGATCGTTGCCCCTCCGGAACAGCAGCTTAACCAATAGCAGACGAAGTGTCCAAGTTG *F TCCGGCGCCGATCGCAATCTGTGGGCGGTAGTCTAGTAAAAACAATCACCGCAGCAGACCGCGAGGTGAAGAAATCGCCA *F GCGAAGCCTGGAGGTGGAGCAGGACGACCGAAAAGTTCCCCGGGAAAAAATAGCTCCATTATAGCGAAAAGGGGAAGCGC *F CCCACCAAAAAGCGCCTCACCGACCAAGCGGCTGGGCAATCGCAAGAAGCCCTAGCAATTCGGGTAGTCAGTTATGCAAG *F TGCCTTAACAAAGACTGCGAGGAAATCGAGGGACCAAGCCTCTAGCACCAGTAGCTAACCTAATTACTAACCCAACTAGC *F AAGTGCCTTCGTTCCCTGTTTAATTTCGCCTCTAATCCCTGCCAGAAATATGTCGAGCTGAAACAGGAAGAGCGCCAGTG *F GGCGGCCACACGTATTAATGCCGGTGTGCGAGGATTTTTGGTCCGCCGGCTTTTTGCAACCGAGCAAGTGCAGCGCATTG *F TCCAAACCATCAGAGACACCCTGATCTTCGTGTTGAACCTGCACCTGGAAACTTGTGGACATGGCCTGGATGCAGAGGAG *F CCTGCAAACTTGCGCCTCAAGGCGCGATTGCTTCAACAGAACAGCTGTTTGGCAAAAGCCCGCGTGATTTTTTGCAGCCA *F AGCTTTCAAGACATTGCGGAGCTTTCTAGACAAATTCGCCCTGAAAGCTGCGGCGGATTGCCAAAACAAACGGAACTTTT *F AAAGCGAGTAGTCGTGTGTGCGTGTGATGGCCTTAAAAGAAAACAGCAAAGGCAGACAAATAATCAAAAGCGAGAGAAAA *F CTAGTTTAATAACAATTAATTATTAGTGAGCACGTAACCCACAGCAATTGGCCGACCTCCCGCTCATCCTGTTATTCACC *F CAGCTGCCCGCGTGCCTTGCCCACCCAATTCCATAATTCCGCCGGCGGCGAATTTAGAACGACACACCAAATTGAAAAAA *F TAATAATACAAATAATAAAACCACACCAATAACAGAAAAAAAAAAAAAAAAAA *F >AT11309 *F GCGATCCACGGTAGAAACATTGCATACAAAACTCTCAACGATTCGGGATATCATTTGATTGATGTAATGAACTCTTCAGA *F GGTTTCCTAATTTTTGGCGGGAAGTCGAGGGCTCCCTAGTGCCATCGTCATCTCTAATGGCAACTAAAACATAGCAGACA *F TTTAATGAACTTTCACAAAACACCTAGATTCAGCTGACCTCTGAGAAGGCTTCCTGACATTTCGACGTCCCAACAAAAAT *F TCCCAGTAGCAAAACACCAATGGATGCGACGTGGGCAATGGAGCAGCTGCTGTCTGCAGTTGAGATGGGAGGTAGCCACA *F GCGAGGCCACCACACCAACGGCCACGACCACCATATCGATCTGGAGCGCCCCCTCCGAGGGCAGTGGCTCTAGTTCCATG *F GACCCTGTCTCCTTTCGGTTCCACTTGGACGGGCAGCCTATACTGCCACCGCTGATGACGAGCGCCAAGAAGCGCGAGGT *F GCAGCTGGCCCGCCAGATGGCAGAAGCGCTGGAGAAGCGGTACCGCCTGGCCAGACACTCGGCGGGATCCGACATGGCCA *F GTAGGAGAAGTGTGTCACAGCTGCAGCAGACAGAAACGTTAATCTATGACAGCACACGGGGCCAGGCTCGTCCTCAGACT *F CTGGTCTTTGGCAAACAGTTGCCCACCATACAGGTGGATCCTCCCACGCCTCAACCATTTGTGAAATCAATAGAAAACAC *F TTCCCCAAGGCGGCATAATCGCATTACCGATCGTATCCTGCAGTTCGAGCAGTCGGGACTGGGTAAGCTGCTGCCCAAAG *F TTCCAGATAAGTGTATCCTGCGCACAAGTCCTGCGGTAGCCGAAGACCAGCAGGATCAGGATACAAAGGACACAACGGCA *F AGGGAGCTCGGCAGTCCACTGGCAGAACCAGCTAATTTTCAGCGTTCGAGAAGTTTCACACTAGAGGAGCCCTCCCAGGT *F TCTCGTGGAGCACATGGAGAGGGAGGCCCTGGCTGTGAAACCCAGCCAGAGTTTGGCCAACTTCGAGAAGCAGACCATCG *F AGTCTCAGGCCAAGCGGGTGAACCGTAGTGCAAGGAGTATTAGCAGCAAAATTAGCCACAACAGCTACAATAGCAACAGT *F AGCGCCGCAACCAAGAGCACCATCGCCACTAAAACCACAACCGCCGGCAGCAGCTGCTCTAGGCGGGATCGCGAGGTGGA *F GCGTATAATAGAACGGGCGCTGGACGAGCATGGCGCTTTGGAAGCCAGCCGCAAGGCAGGAGTGCGCAACTATCTGCGTG *F GCCATCGGGAGCGAATGAATCAATTGGTCATTCACCAGGAGAAGGAACGTCGTCGCATGCAGGCCGAGTTTGATCATCAG *F CAGCGTCAGCTCATAGAGGAACTGTGTGCCGAAATTGATGTTTCCTCGGCCACCGAAAATCCTGACAGTCTGATATCGCA *F AAGCACCAGTACGGGAGACCTGCAGCGTTTTTCCCCCTCGCCTACGTTACCCTCCTTTTCAGAGGACATAGAAGACTTTC *F CGACGATGGAAGACAAGTGCCAAATTGCGGGGCCTTCTTCCGCTCGCAAGAGGCTGTTTAGTCCAAAACCATCAAAGGAC *F TCGGAACCGCAGCTGCTCAGTGCCCCCAGCACACCGCGATCGTTGCCCCTCCGGAACAGCAGCTTAACCAATAGCAGACG *F AAGTGTCCAAGTTGTCCGGCGCCGATCGCAATCTGTGGGCGGTAGTCTAGTAAAAACAATCACCGCAGCAGACCGCGAGG *F TGAAGAAATCGCCAGCGAAGCCTGGAGGTGGAGCAGGACGACCGAAGAGTTCCCCGGGAAAAAATAGCTCCATTATAGCG *F AAAAGGGGAAACGCCCCACCAAAAAGCGCCTCACCGACCAAGCGGCTGGGCAATCGCAAGAAGCCCTAGCAATTCGGGTA *F GTCAGTTATGCAAGTGCCTTAACAAAGACTGCGAGGAAATCGAGGGACCAAGCCTCTAGCACCAGTAGCTAACCTAATTA *F CTAACCCAACTAGCAAGTGCCTTCGTTCCCTGTTTAATTTCGCCTCTAATCCCTGCCAGAAATATGTCGAGCTGAAACAG *F GAAGAGCGCCAGTGGGCGGCCACACGTATTAATGCCGGTGTGCGAGGATTTTTGGTCCGCCGGCTTTTTGCAACCGAGCA *F AGTGCAGCGCATTGTCCAAACCATCAGAGACACCCTGATCTTCGTGTTGAACCTGCACCTGGAAACTTGTGGACATGGCC *F TGGATGCAGAGGAGCCTGCAAACTTGCGCCTCAAGGCGCGATTGCTTCAACAGGTGGGTTACTTGGTTACTTGTCTAGGT *F GTAAAAGGTGCCAAATGTGTACTTTAGGCTTGGATGATAAAACTAGAAATAAATTATTATCTGAGTGAAGGGTATGCGAT *F AGTCAAAAAAAAAAAAAAAAAAAAAAAAAAA *F >RE58503 *F TCCCCGCGGTGGCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGA *F ATTCGTCGAGAGCGGGTTTTGAATTGCCGTTTGAAAATAAATGTTTTCCCCAGCATTAAAGCAGCATTTCTCATTGGCTC *F TTGATGGTAGCTGCGATCCACGGTAGAAACATTGAATACAAAACTCTCAACGATTCGGGATATCATTTGATTGATATTCA *F GCTGACCTCTGAGAAGGCTTCCTGACATTTCGACGTCCCAACAAAAATTCCCAGTAGCAAAACACCAATGGATGCGACGT *F GGGCAATGGAGCAGCTGCTGTCTGCAGTTGAGATGGGAGGTAGCCACAGCGAGGCCACCACACCAACGGCCACGACCACC *F ATATCGATCTGGAGCGCCCCCTCCGAGGGCAGTGGCTCTAGTTCCATGGACCCTGTCTCCTTTCGGTTCCACTTGGACGG *F GCAGCCTATACTGCCACCGCTGATGACGAGCGCCAAGAAGCGCGAGGTGCAGCTGGCCCGCCAGATGGCAGAAGCGCTGG *F AGAAGCGGTACCGCCTGGCCAGACACTCGGCGGGATCCGACATGGCCAGTAGGAGAAGTGTGTCACAGCTGCAGCAGACA *F GAAACGTTAATCTATGACAGCACACGGGGCCAGGCTCGTCCTCAGACTCTGGTCTTTGGCAAACAGTTGCCCACCATACA *F GGTGGATCCTCCCACGCCTCAACCATTTGTGAAATCAATAGAAAACACTTCCCCAAGGCGGCATAATCGCATTACCGATC *F GTATCCTGCAGTTCGAGCAGTCGGGACTGGGTAAGCTGCTGCCCAAAGTTCCAGATAAGTGTATCCTGCGCACAAGTCCT *F GCGGTAGCCGAAGACCAGCAGGATCAGGATACAAAGGACACAACGGCAAGGGAGCTCGGCAGTCCACTGGCAGAACCAGC *F TAATTTTCAGCGTTCGAGAAGTTTCACACTAGAGGAGCCCTCCCAGGTTCTCGTGGGCACATGGAGAGGGAGGCCCTGGC *F TGTGAAACCCAGCCAGAGTTTGGCCAACTTCGAGAAGCAGACCATCGAGTCTCAGGCCAAGCGGGTGAACCGTAGTGCAA *F GGAGTATTAGCAGCAAAATTAGCCACAACAGCTACAATAGCAACAGTAGCGCCGCAACCAAGAGCACCATCGCCACTAAA *F ACCACAACCGCCGGCAGCAGCTGTTCTAGGCGGGATCGCGAGGTGGAGCGTATAATAGAACGGGCGCTGGACGAGCATGG *F CGCTTTGGAAGCCAGCCGCAAGGCAGGAGTGCGCAACTATCTGCGTGGCCATCGGGAGCGAATGAATCAATTGGTCATTC *F ACCAGGAGAAGGAACGTCGTCGCATGCAGGCCGAGTTTGATCATCAGCAGCGTCAGCTCATAGAGGAACTGTGTGCCGAA *F ATTGATGTTTCCTCGGCCACCGAAAATCCTGACAGTCTGATATCGCAAAGCACCAGTACGGGAGACCTGCAGCGTTTTTC *F CCCCTCGCCTACGTTACCCTCCTTTTCAGAGGACATAGAAGACTTTCCGACGATGGAAGACAAGTGCCAAATTGCGGGGC *F CTTCTTCCGCTCGCAAGAGGCTGTTTAGTCCAAAACCATCAAAGGACTCGGAACCGCAGCTGCTCAGTGCCCCCAGCACA *F CCGCGATCGTTGCCCCTCCGGAACAGCAGCTTAACCAATAGCAGACGAAGTGTCCAAGTTGTCCGGCGCCGATCGCAATC *F TGTGGGCGGTAGTCTAGTAAAAACAATCACCGCAGCAGACCGCGAGGTGAAGAAATCGCCAGCGAAGCCTGGAGGTGGAG *F CAGGACGACCGAAAAGTTCCCCGGGAAAAAATAGCTCCATTATAGCGAAAAGGGGAAGCGCCCCACCAAAAAGCGCCTCA *F CCGACCAAGCGGCTGGGCAATAAATATGTCGAGCTGAAACAGGAAGAGCGCCAGTGGGCGGCCACACGTATTAATGCCGG *F TGTGCGAGGATTTTTGGTCCGCCGGCTTTTTGCAACCGAGCAAGTGCAGCGCATTGTCCAAACCATCAGAGACACCCTGA *F TCTTCGTGTTGAACCTGCACCTGGAAACTTGTGGACATGGCCTGGATGCAGAGGAGCCTGCAAACTTGCGCCTCAAGGCG *F CGATTGCTTCAACAGCTCTGCTCCGCCAGTCGTACCCTGCACCTGATCTTCTTCCAGACCAACATAAAGGACCGCATGGA *F GATCATCGCTCGGGATCGCCAGAGGATCAAGACCAAACTACTCCTCAAACATCGCCGATTGGATTAGGATTTCATAGAAC *F AGCTGTTTGGCAAAAGCCCGCGTGATTTTTTGCAGCCAAGCTTTCAAGACATTGCGGAGCTTTCTAGACAAATTCGCCCT *F GAAAGCTGCGGCGGATTGCCAAAACAAACGGAACTTTTAAAGCGAGTAGTCGTGTGTGCGTGTGATGGCCTTAAAAGAAA *F ACAGCAAAGGCAGACAAATAATCAAAAGCGAGAGAAAACTAGTTTAATAACAATTAATTATTAGTGAGCACGTAACCCAC *F AGCAATTGGCCGACCTCCCGCTCATCCTGTTATTCACCCAGCTGCCCGCGTGCCTAA *F >RE33938 *F AATGGCAACTAAAACATAGCAGACATTTAATGAACTTTCACAAAACACCTAGATTCAGCTGACCTCTGAGAAGGCTTCCT *F GACATTTCGACGTCCCAACAAAAATTCCCAGTAGCAAAACACCAATGGATGCGACGTGGGCAATGGAGCAGCTGCTGTCT *F GCAGTTGAGATGGGAGGTAGCCACAGCGAGGCCACCACACCAACGGCCACGACCACCATATCGATCTGGAGCGCCCCCTC *F CGAGGGCAGTGGCTCTAGTTCCATGGACCCTGTCTCCTTTCGGTTCCACTTGGACGGGCAGCCTATACTGCCACCGCTGA *F TGACGAGCGCCAAGAAGCGCGAGGTGCAGCTGGCCCGCCAGATGGCAGAAGCGCTGGAGAAGCGGTACCGCCTGGCCAGA *F CACTCGGCGGGATCCGACATGGCCAGTAGGAGAAGTGTGTCACAGCTGCAGCAGACAGAAACGTTAATCTATGACAGCAC *F ACGGGGCCAGGCTCGTCCTCAGACTCTGGTCTTTGGCAAACAGTTGCCCACCATACAGGTGGATCCTCCCACGCCTCAAC *F CATTTGTGAAATCAATAGAAAACACTTCCCCAAGGCGGCATAATCGCATTACCGATCGTATCCTGCAGTTCGAGCAGTCG *F GGACTGGGTAAGCTGCTGCCCAAAGTTCCAGATAAGTGTATCCTGCGCACAAGTCCTGCGGTAGCCGAAGACCAGCAGGA *F TCAGGATACAAAGGACACAACGGCAAGGGAGCTCGGCAGTCCACTGGCAGAACCAGCTAATTTTCAGCGTTCGAGAAGTT *F TCACACTAGAGGAGCCCTCCCAGGTTCTCGTGGAGCACATGGAGAGGGAGGCCCTGGCTGTGAAACCCAGCCAGAGTTTG *F GCCAACTTCGAGAAGCAGACCATCGAGTCTCAGGCCAAGCGGGTGAACCGTANTGCAAGGAGTATTAGCAGCAAAATTAG *F CCACAACAGCTACAATAGCAACAGTAGCGCCGCAACCAAGAGCACCATCGCCACTAAAACCACACCCGCCGGCAGCAGCT *F GTTCTAGGCGGGATCGCGAGGTGGANCGTATAATAGAACGGGCGCTGGACGAGCATGGCGCTTTGGAAGCCAGCCGCAAG *F GCAGGAGTGCGCAACTATCTGCGTGGCCATCGGGAGCGAATGAATCAATTGGTCATTCACCANGAGAAGGAACGTCGTCG *F CATGCANGCCGAGTTTGATCATCAGCAGCGTCAGCTCATANAGGAACTGTGTGCCGAAATTGATGTTTCCTCGGCCACCG *F AAAATCCTGACAGTCTGATATCGCAAAGCACCAGTACGGGAGACCTGCAGCGTTTTTCCCCCTCGCCTACGTTACCCTCC *F TTTTCAGAGGACATAGAAGACTTTCCGACGATGGAAGACAAGTGCCAAATTGCGGGGCCTTCTTCCGCTCGCAAGAGGCT *F GTTTAGTCCAAAACCATCAAAGGACTCGGAACCGCAGCTGCTCAGTGCCCCCAGCACACCGCGATCGTTGCCCCTCCGGA *F ACAGCAGCTTAACCAATAGCAGACGAAGTGTCCAAGTTGTCCGGCGCCGATCGCAATCTGTGGGCGGTAGTCTAGTAAAA *F ACAATCACCGCAGCAGACCGCGAGGTGAAGAAATCGCCAGCGAAGCCTGGAGGTGGAGCAGGACGACCGAAAAGTTCCCC *F GGGAAAAAATAGCTCCATTATAGCGAAAAGGGGAAGCGCCCCACCAAAAAGCGCCTCACCGACCAAGCGGCTGGGCAATA *F AATATGTCGAGCTGAAACAGGAAGAGCGCCAGTGGGCGGCCACACGTATTAATGCCGGTGTGCGAGGATTTTTGGTCCGC *F CGGCTTTTTGCAACCGAGCAAGTGCAGCGCATTGTCCAAACCATCAGAGACACCCTGATCTTCGTGTTGAACCTGCACCT *F GGAAACTTGTGGACATGGCCTGGATGCAGAGGAGCCTGCAAACTTGCGCCTCAAGGCGCGATTGCTTCAACAGCTCTGCT *F CCGCCAGTCGTACCCTGCACCTGATCTTCTTCCAGACCAACATAAAGGACCGCATGGAGATCATCGCTCGGGATCGCCAG *F AGGATCAAGACCAAACTACTCCTCAAACATCGCCGATTGGATTAGGATTTCATAGGTTAGATTGCTCGAATAAAGGAGGA *F AACGATAAACGAAAAAAAAAAAAAAAA *F >AT23168 *F CTCAACGATTCGGGATATCATTTGATTGATGTAATGAACTCTTCAGAGGTTTCCTAATTTTTGGCGGGAAGTCGAGGGCT *F CCCTAGTGCCATCGTCATCTCTAATGGCAACTAAAACATAGCAGACATTTAATGAACTTTCACAAAACACCTAGATTCAG *F CTGACCTCTGAGAAGGCTTCCTGACATTTCGACGTCCCAACAAAAATTCCCAGTAGCAAAACACCAATGGATGCGACGTG *F GGCAATGGAGCAGCTGCTGTCTGCAGTTGAGATGGGAGGTAGCCACAGCGAGGCCACCACACCAACGGCCACGACCACCA *F TATCGATCTGGAGCGCCCCCTCCGAGGGCAGTGGCTCTAGTTCCATGGACCCTGTCTCCTTTCGGTTCCACTTGGACGGG *F CAGCCTATACTGCCACCGCTGATGACGAGCGCCAAGAAGCGCGAGGTGCAGCTGGCCCGCCAGATGGCAGAAGCGCTGGA *F GAAGCGGTACCGCCTGGCCAGACACTCGGCGGGATCCGACATGGCCAGTAGGAGAAGTGTGTCACAGCTGCAGCAGACAG *F AAACGTTAATCTATGACAGCACACGGGGCCAGGCTCGTCCTCAGACTCTGGTCTTTGGCAAACAGTTGCCCACCATACAG *F GTGGATCCTCCCACGCCTCAACCATTTGTGAAATCAATAGAAAACACTTCCCCAAGGCGGCATAATCGCATTACCGATCG *F TATCCTGCAGTTCGAGCAGTCGGGACTGGGTAAGCTGCTGCCCAAAGTTCCAGATAAGTGTATCCTGCGCACAAGTCCTG *F CGGTAGCCGAAGACCAGCAGGATCAGGATACAAAGGACACAACGGCAAGGGAGCTCGGCAGTCCACTGGCAGAACCAGCT *F AATTTTCAGCGTTCGAGAAGTTTCACACTAGAGGAGCCCTCCCAGGTTCTCGTGGAGCACATGGAGAGGGAGGCCCTGGC *F TGTGAAACCCAGCCAGAGTTTGGCCAACTTCGAGAAGCAGACCATCGAGTCTCAGGCCAAGCGGGTGAACCGTAGTGCAA *F GGAGTATTAGCAGCAAAATTAGCCACAACAGCTACAATAGCAACAGTAGCGCCGCAACCAAGAGCACCATCGCCACTAAA *F ACCACAACCGCCGGCAGCAGCTGCTCTAGGCGGGATCGCGAGGTGGAGCGTATAATAGAACGGGCGCTGGACGAGCATGG *F CGCTTTGGAAGCCAGCCGCAAGGCAGGAGTGCGCAACTATCTGCGTGGCCATCGGGAGCGAATGAATCAATTGGTCATTC *F ACCAGGAGAAGGAACGTCGTCGCATGCAGGCCGAGTTTGATCATCAGCAGCGTCAGCTCATAGAGGAACTGTGTGCCGAA *F ATTGATGTTTCCTCGGCCACCGAAAATCCTGACAGTCTGATATCGCAAAGCACCAGTACGGGAGACCTGCAGCGTTTTTC *F CCCCTCGCCTACGTTACCCTCCTTTTCAGAGGACATAGAAGACTTTCCGACGATGGAAGACAAGTGCCAAATTGCGGGGC *F CTTCTTCCGCTCGCAAGAGGCTGTTTAGTCCAAAACCATCAAAGGACTCGGAACCGCAGCTGCTCAGTGCCCCCAGCACA *F CCGCGATCGTTGCCCCTCCGGAACAGCAGCTTAACCAATAGCAGACGAAGTGTCCAAGTTGTCCGGCGCCGATCGCAATC *F TGTGGGCGGTAGTCTAGTAAAAACAATCACCGCAGCAGACCGCGAGGTGAAGAAATCGCCAGCGAAGCCTGGAGGTGGAG *F CAGGACGACCGAAGAGTTCCCCGGGAAAAAATAGCTCCATTATAGCGAAAAGGGGAAACGCCCCACCAAAAAGCGCCTCA *F CCGACCAAGCGGCTGGGCAATCGCAAGAAGCCCTAGCAATTCGGGTAGTCAGTTATGCAAGTGCCTTAACAAAGACTGCG *F AGGAAATCGAGGGACCAAGCCTCTAGCACCAGTAGCTAACCTAATTACTAACCCAACTAGCAAGTGCCTTCGTTCCCTGT *F TTAATTTCGCCTCTAATCCCTGCCAGAAATATGTCGAGCTGAAACAGGAAGAGCGCCAGTGGGCGGCCACACGTATTAAT *F GCCGGTGTGCGAGGATTTTTGGTCCGCCGGCTTTTTGCAACCGAGCAAGTGCAGCGCATTGTCCAAACCATCAGAGACAC *F CCTGATCTTCGTGTTGAACCTGCACCTGGAAACTTGTGGACATGGCCTGGATGCAGAGGAGCCTGCAAACTTGCGCCTCA *F AGGCGCGATTGCTTCAACAGCTCTGCTCCGCCAGTCGTACCCTGCACCTGATCTTCTTCCAGACCAACATAAAGGACCGC *F ATGGAGATCATCGCTCGGGATCGCCAGAGGATCAAGACCAAACTACTCCTCAAACATCGCCGATTGGATTAGGATTTCAT *F AGAACAGCTGTTTGGCAAAAGCCCGCGTGATTTTTTGCAACCAAGCTTTCAAGACATTGCACAGCTTTCTAGACAAATTC *F GCCCTGAAAGCTGCGGCGGATTGCCAAAACAAACGGAACTTTTGAAGCGAGTAGTCGTGTGTGCGTGTGATGGCCTTAAA *F AGAAAACAGCAAAGGCAGACAAATAATCAAAAGCGAGAGAAAACTAGTTTAATAACAATTAATTATTAGTGAGCACGTAA *F CCCACAGCAATTGGCCGACCTCCCGCTCATCCTGTTATTCACCCAGCTGTCCGCGTGCCTTGCCCACCCAATTCCATAAT *F TCCGCCGGCGGCGAATTTAGAACGACACACCAAATTGAAAAAATAATAATACAAATAATAAAACCACAAAAAAAAAAAAA *F AAAAA # *U FBrf0188548 *a Robertson *b H. *t 2005.7.7 *T personal communication to FlyBase *u FlyBase error report for CG32402 on Thu Jul 7 21:41:59 2005. *F Date: Thu, 7 Jul 2005 21:41:59 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG32402 on Thu Jul 7 21:41:59 2005 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG32402 *F Gene annotation error *F Gene CG32402 has incorrect exon/intron structure. *F Comments: By comparison with D. pseudoobscura orthologs it is clear that a *F nearby upstream inframe start codon should be employed, lengthening the *F predicted protein by 21aa. MDIQRFLKFYKVGWKTYRDPL # *U FBrf0188549 *a Robertson *b H. *t 2005.7.7 *T personal communication to FlyBase *u FlyBase error report for CG32403 on Thu Jul 7 21:44:27 2005. *F Date: Thu, 7 Jul 2005 21:44:27 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: hughrobe@uiuc.edu *F Subject: FlyBase error report for CG32403 on Thu Jul 7 21:44:27 2005 *F Error report from Hugh Robertson (hughrobe@uiuc.edu) *F Gene or accession: CG32403 *F Gene annotation error *F Gene CG32403 has incorrect exon/intron structure. *F Comments: By comparison with D. pseudoobscura orthologs there is a nearby *F upstream inframe start codon that should be used, adding 25aa \- *F MDIRGNVHRFVKFYIDGWKHFRDPT # *U FBrf0188550 *a Murphy *b T. *t 2005.7.29 *T personal communication to FlyBase *u FlyBase error report for CG3980 on Fri Jul 29 13:56:50 2005. *F Date: Fri, 29 Jul 2005 13:56:50 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: tmurphy@ciwemb.edu *F Subject: FlyBase error report for CG3980 on Fri Jul 29 13:56:50 2005 *F Error report from Terence Murphy (tmurphy@ciwemb.edu) *F Gene or accession: CG3980 *F cDNA or EST error *F Comments: I have recently assembled full-length sequences of 4 cDNAs *F corresponding to CG3980, which reveal an un-annotated intron. FASTA formatted *F sequences are attached below. These sequences have also been submitted to *F Genbank. *F >AT07330 *F ACAATAAACAAAAATTCACTGCATTGCACGGCGCTAAAATAAAAATTAAGTTTGCAAATCATGGCGTGGACTGAATTATA *F AACCCGGCTTGAAACCAAAGGACTGCAAGTGGAACAGCTGAGCTGCACACTTGGGAAATTGCCAATCTGAAACTGCGTGG *F AAAAAAGAGTGGCCCATTGATTTTTCGCTAAGTATCTGCTGATTAAAAAAAAATTGACCGCAGCGTTGGAATTTCGGCCT *F GCCAATCAGAAAAATGAGTGGCGACGAAAGTGGCGAGGAGAAGCACGTCCTGAACTTGTCCAAGCAAAAGCTGAAGAAGG *F TGCCCAAGCAGGACGATGCGCACAGCATACGGCAGCTCATCCTGGACGAGAACGAGCTGCAGAAGATCGACAACATAGAC *F TCGTATCTCAAAATTGAAACGCTCTCCTTGGCCAGAAATCAATTGCTGCGCATGTATGGCGTCTGCCGGCTGCATTGCCT *F GCGTGAACTGAATCTGTCCTTCAATGGCATACTCTCTATCGAGGGCCTGAAGGAATGCATCCATCTGCGCGTTCTGAATC *F TGGAGGGGAATAACATCAAGACCATCGAGCATCTGAACACGAATGTCAATCTGGAGTGCCTGAATCTGGCGGATAATAGC *F ATTGGCAGCATTTCGGACATGTCCTATTTGCGCAATCTCAAGGAGCTATATTTGCACGGAAATCGCTTGACGCATCTGAG *F GCAGTGCGACAAGTGTTTGCCCACCTCACTGGAAACCCTAACGTTGGCCAAGAATAGCATCAACGATCTCAACGAGATCT *F GTACGCTGTCGCATCTGAGCAACTTGCTCAGCATTTCCATTGCGGATAATCCCTGTGTGACCATGATCAACAGTTTGGAT *F GGCTTTGATTACCGACCTTTTGTTTTAAACTGGTGCATGAGCCTCAAGTACATCGATGGCTTTGTGGTCGATCCTATTGA *F GAGTCTTAAGGCCGAATGGCTGTATAGCCAGGGACGTGGTCGCCAGTTTCGTGTGGGCGAGCAGCAGGGCTTGGCAAAAT *F ACCTAAGTTCTGTGTGCCCGCTGGTGGGCAAGGCGCTGGAAAATGAAAACGATAGGAAACTCCGGCTGATCCTCAGCAAG *F GCGCAACACCATCAGCGGCAGCTGCAGGAAGAGATCATGGATAACGCAAATAGTTCTGCCAGCACTTCGCCCTCATCCCA *F TCGCAAGAAGCCCACGAGTCGCATTCAGTCGCCCAGATTCTCCCGCTTGAGTGGCCGCCAGGGATCACCGGAGTCCATGG *F TGAATAGCTATCACGGGAACAGTAGCAACAATAGCATTGTTAGCGACAATGGATCTACGAATCATTCGCTGCAAATGAGC *F ATTTCGCTTATTGAGAACATTAAAAACGATGGCGAAGGCTTTAGTTTGGCCGGCAGCGGAATGTCCAGCAGCGTGACCAC *F CAAGACATACAATTCCACGGAGTCCACGCCACGGAATATAACACCCAATCCCTACAATGATCAAACGTTCATCAGTCAAA *F CCCCATCTGGTGGTCCATTGGCGGCTGCCTCGAAAATGGTCCCAGTTCCGGAGACTCTGATGAGTCCGGATGTCTGTCCC *F GCCGTTGTGGCCCAAAGAGTTACTGTTACGGCACTGAACCCTCAGCTGCACAAGACCAAAAAAAAAAAAAAAAAAA *F >RE44293 *F CGTAGTATAATAACAATAAACAAAAATTCACTGTATTGCACGGCGCTAAAATAAAAATTAAGTTTGCAAATCATGGCGTG *F GACTGAATTATAAACCCGGCTTGAAACCAAAGGACTGCAAGTGGAACAGCTGAGCTGCACACTTGGGAAATTGCCAATCT *F GAAACTGCGTGGAAAAAAGAGTGGCCCATTGATTTTTCGCTAAGTATCTGCTGATTAAAAAAAAATTGACCGCAGCGTTG *F GAATTTCGGCCTGCCAATCAGAAAAATGAGTGGCGACGAAAGTGGCGAGGAGAAGCACGTCCTGAACTTGTCCAAGCAAA *F AGCTGAAGAAGGTGCCCAAGCAGGACGATGCGCACAGCATACGGCAGCTCATCCTGGACGAGAACGAGCTGCAGAAGATC *F GACAACATAGACTCGTATCTCAAAATTGAAACGCTCTCCTTGGCCAGAAATCAATTGCTGCGCATGTATGGCGTCTGCCG *F GCTGCATTGCCTGCGTGAACTGAATCTGTCCTTCAATGGCATACTCTCTATCGAGGGCCTGAAGGAATGCATCCATCTGC *F GCGTTCTGAATCTGGAGGGGAATAACATCAAGACCATCGAGCATCTGAACACGAATGTCAATCTGGAGTGCCTGAATCTG *F GCGGATAATAGCATTGGCAGCATTTCGGATATGTCCTATTTGCGCAATCTCAAGGAGCTATATTTGCACGGAAATCGCTT *F GACGCATCTGAGGCAGTGCGACAAGTGTTTGCCCACCTCACTGGAAACCCTAACGTTGGCCAAGAATAGCATCAACGATC *F TCAACGAGATCTGTACGCTGTCGCATCTGAGCAACTTGCTGAGCATTTCCATTGCGGATAATCCCTGTGTGACCATGATC *F AACAGTTTGGATGGCTTTGATTACCGACCTTTTGTTTTAAACTGGTGCATGAGCCTCAAGTACATCGATGGCTTTGTGGT *F CGATCCTATTGAGAGTCTTAAGGCCGAATGGCTGTATAGCCAGGGACGTGGTCGCCAGTTCCGTGTGGGCGAGCAGCAGG *F GCTTGGCAAAATACCTAAGTTCTGTGTGCCCGCTGGTGGGCAAGGCGCTGGAAAATGAAAACGATAGGAAACTCCGGCTG *F ATCCTCAGCAAGGCGCAACACCATCAGCGGCAGCTGCAGGAAGAGATCATGGATAACGCAAATAGTTCTGCCAGCACTTC *F GCCCTCATCCCATCGCAAGAAGCCCACGAGTCGCATTCAGTCGCCCAGATTCTCCCGCTTGAGTGGCCGCCAGGGATCAC *F CGGAGTCCATGGTGAATAGCTATCACGGGAACAGCAGCAACAATAGCATTGTTAGCGACAATGGATCTACGAATCATTCG *F CTGCAAATGAGCATTTCGCTCATTGAGAACATTAAAAACGATGGCGAAGGCTTTAGTTTGGCCGGCAGCGGAATGTCCAG *F CAGCGTGACCACCAAGACATACAATTCCACGGAGTCCACGCCACGGAATATAACACCCAATCCCTACAATGATCAAACGT *F TCATCAGTCAAACCCCATCTGGTGGTCCATTGGCGGCTGCCTCGAAAATGGTCCCAGTTCCGGAGACTCTGATGAGCCCG *F GATGTCTGTCCCGCCGCTGTGGCCCAAAGAGTTACGGTTACGGCACTGAACCCTCAGCTGCACAAGACCAAGATCAACAA *F AAATAAAGACAACAAACTGAACTTGCCAAGAGTGCGAAGTCCACAACTGAAGAGGAACCAAAGCCCTACGAGCAGTCCAC *F GGAGGATGGCCAATAAGTGCAGCGCGGACAACATGCAGGCACCGGAGAAACTCCAGCAATCGGCTGTTTTGGCCGATAGC *F GGTGGCTTCAGCACCGACGACGACGGCGAGCAAATCAACATAGAGAAACTGAGAGCAATCCGGAAGATGGCCGCTCAGAA *F GCAACAGCAGATGCATCAGGAGCAGCTGAAGCAGCAGGTGGACAACAACCTGAACTGCCAGGATCGGCTGATCGAGCACG *F TGACAGAGTCCTCGGCCGTTACCATCCAAAAGATGTGGCGAGGCTATCACACACGCAAGAAGACCAACAAGGACATAGCC *F GAGCGATTGCAGCGACGACGCACACAGGAGTATATCGAACAACTCGGCAAGGATATGCTACTTACGAAGGCCCAGCTCGA *F GAACGAGCGAAAGATTCAGCAGCTGCAGATGCAGGCCATCAATGCGCTGTGGAAAAAGGTGTCCACCATGGAGGTGGATC *F CCAAGGGAATACCTGCTGCTGCGGAACAGGGCGGGGACTCCAATGGCGGAGGGTCTGGCCACCTGAGTCTCGATCAAAAT *F TCAGCCGCCGTGGTAAATGATTTGGCGAAAAGATGCACCATGCTCACCGATCAGGTTCAAATGCTGCAAAGCTCGATTGG *F AACCATCGTCAACTGCCTCACAATGGTGTGCAATCTGCCGCAGGACGCTATTAAGAAGCAGGCCGAGATCATTGATTGCA *F GCTCCACTCAGACGGACCTCATTGCCGTACACACGCCGCAAATAGAGGACCTCACCAATTTTCCATTCACCAAAACAAGG *F CCCTCAACCCTGGCCCTAGAATCCAAGCACGAAGCTGCTCTGGCTTGTCCCAAAATCGATGAATTGAATGATGTCGACCT *F GAAGGAGACCCAAGAAGACTCGGAAAATCTTGATAAAGATCCATGAACAAATCCCAGTTAATAAATGAGGAGTATAAACG *F CTTTAAGGGTCGATCTCAGCGCTTATTAATTCCTTTCTTAGGCTTTTGTGTAGCTTTTGCAACAATATTAGATCGGTTAC *F GAGAACTTAGAAGTAGCACCAGCATTCACAAACACATTACTTTGTTACATTCCTGAGATGCAGCGATGGTGGAAGACTTA *F ACAATAGTATGAACAAATCGTAAATACAAATTTTTGTAAAGCAAAAAAAAAAAAAAAA *F >RE26466 *F GTCGAGAGCGGAGACGTAGTATAATAACAATAAACAAAAATTCACTGTATTGCACGGCGCTAAAATAAAAATTAAGTTTG *F CAAATCATGGCGTGGACTGAATTATAAACCCGGCTTGAAACCAAAGGACTGCAAGTGGAACAGCTGAGCTGCACACTTGG *F GAAATTGCCAATCTGAAACTGCGTGGAAAAAAGAGTGGCCCATTGATTTTTCGCTAAGTATCTGCTGATTAAAAAAAAAT *F TGACCGCAGCGTTGGAATTTCGGCCTGCCAATCAGAAAAATGAGTGGCGACGAAAGTGGCGAGGAGAAGCACGTCCTGAA *F CTTGTCCAAGCAAAAGCTGAAGAAGGTGCCCAAGCAGGACGATGCGCACAGCATACGGCAGCTCATCCTGGACGAGAACG *F AGCTGCAGAAGATCGACAACATAGACTCGTATCTCAAAATTGAAACGCTCTCCTTGGCCAGAAATCAATTGCTGCGCATG *F TATGGCGTCTGCCGGCTGCATTGCCTGCGTGAACTGAATCTGTCCTTCAATGGCATACTCTCTATCGAGGGCCTGAAGGA *F ATGCATCCATCTGCGCGTTCTGAATCTGGAGGGGAATAACATCAAGACCATCGAGCATCTGAACACGAATGTCAATCTGG *F AGTGCCTGAATCTGGCGGATAATAGCATTGGCAGCATTTCGGATATGTCCTATTTGCGCAATCTCAAGGAGCTATATTTG *F CACGGAAATCGCTTGACGCATCTGAGGCAGTGCGACAAGTGTTTGCCCACCTCACTGGAAACCCTAACGTTGGCCAAGAA *F TAGCATCAACGATCTCAACGAGATCTGTACGCTGTCGCATCTGAGCAACTTGCTGAGCATTTCCATTGCGGATAATCCCT *F GTGTGACCATGATCAACAGTTTGGATGGCTTTGATTACCGACCTTTTGTTTTAAACTGGTGCATGAGCCTCAAGTACATC *F GATGGCTTTGTGGTCGATCCTATTGAGAGTCTTAAGGCCGAATGGCTGTATAGCCAGGGACGTGGTCGCCAGTTCCGTGT *F GGGCGAGCAGCAGGGCTTGGCAAAATACCTAAGTTCTGTGTGCCCGCTGGTGGGCAAGGCGCTGGAAAATGAAAACGATA *F GGAAACTCCGGCTGATCCTCAGCAAGGCGCAACACCATCAGCGGCAGCTGCAGGAAGAGATCATGGATAACGCAAATAGT *F TCTGCCAGCACTTCGCCCTCATCCCATCGCAAGAAGCCCACGAGTCGCATTCAGTCGCCCAGATTCTCCCGCTTGAGTGG *F CCGCCAGGGATCACCGGAGTCCATGGTGAATAGCTATCACGGGAACAGCAGCAACAATAGCATTGTTAGCGACAATGGAT *F CTACGAATCATTCGCTGCAAATGAGCATTTCGCTCATTGAGAACATTAAAAACGATGGCGAAGGCTTTAGTTTGGCCGGC *F AGCGGAATGTCCAGCAGCGTGACCACCAAGACATACAATTCCACGGAGTCCACGCCACGGAATATAACACCCAATCCCTA *F CAATGATCAAACGTTCATCAGTCAAACCCCATCTGGTGGTCCATTGGCGGCTGCCTCGAAAATGGTCCCAGTTCCGGAGA *F CTCTGATGAGCCCGGATGTCTGTCCCGCCGCTGTGGCCCAAAGAGTTACGGTTACGGCACTGAACCCTCAGCTGCACAAG *F ACCAAGATCAACAAAAATAAAGACAACAAACTGAACTTGCCAAGAGTGCGAAGTCCACAACTGAAGAGGAACCAAAGCCC *F TACGAGCAGTCCACGGAGGATGGCCAATAAGTGCAGCGCGGACAACATGCAGGCACCGGAGAAACTCCAGCAATCGGCTG *F TTTTGGCCGATAGCGGTGGCTTCAGCACCGACGACGACGGCGAGCAAATCAACATAGAGAAACTGAGAGCAATCCGGAAG *F ATGGCCGCTCAGAAGCAACAGCAGATGCATCAGGAGCAGCTGAAGCAGCAGGTGGACAACAACCTGAACTGCCAGGATCG *F GCTGATCGAGCACGTGACAGAGTCCTCGGCCGTTACCATCCAAAAGATGTGGCGAGGCTATCACACACGCAAGAAGACCA *F ACAAGGACATAGCCGAGCGATTGCAGCGACGACGCACACAGGAGTATATCGAACAACTCGGCAAGGATATGCTACTTACG *F AAGGCCCAGCTCGAGAACGAGCGAAAGATTCAGCAGCTGCAGATGCAGGCCATCAATGCGCTGTGGAAAAAGGTGTCCAC *F CATGGAGGTGGATCCCAAGGGAATACCTGCTGCTGCGGAACAGGGCGGGGACTCCAATGGCGGAGGGTCTGGCCACCTGA *F GTCTCGATCAAAATTCAGCCGCCGTGGTAAATGATTTGGCGAAAAGATGCACCATGCTCACCGATCAGGTTCAAATGCTG *F CAAAGCTCGATTGGAACCATCGTCAACTGCCTCACAATGGTGTGCAATCTGCCGCAGGACGCTATTAAGAAGCAGGCCGA *F GATCATTGATTGCAGCTCCACTCAGACGGACCTCATTGCCGTACACACGCCGCAAATAGAGGACCTCACCAATTTTCCAT *F TCACCAAAACAAGGCCCTCAACCCTGGCCCTAGAATCCAAGCACGAAGCTGCTCTGGCTTGTCCCAAAATCGATGAATTG *F AATGATGTCGACCTGAAGGAGACCCAAGAAGACTCGGAAAATCTTGATAAAGATCCATGAACAAATCCCAGTTAATAAAT *F GAGGAGTATAAACGCTTTAAGGGTCGATCTCAGCGCTTATTAATTCCTTTCTTAGGCTTTTGTGTAGCTTTTGCAACAAT *F ATTAGATCGGTTACGAGAACTTAGAAGTAGCACCAGCATTCACAAACACATTACTTTGTTACATTCCTGAGATGCAGCGA *F TGGTGGAAGACTTAACAATAGTATGAACAAATCGTAAATACAAATTTTTGTAAAGCAAAAAAAAAAAAAAAA *F >AT18271 *F GATTTCTAAAACATACATACACATTTACACTACAGACAACAAACTGAACTTGCCAAGAGTGCGAAGCCCACAACTGAAGA *F GGAACCAAAGCCCTACGAGCAGTCCACGTAGGATGGCCAACAAGTGCAGCGCGGACAACATGCAGGCACCGGAGAAACTC *F CAGCAATCGGCAGTTTTGGCCGATAGCGGTGGCTTCAGCACCGACGACGACGGCGAGCAGATTAACATAGAGAAACTGAG *F AGCAATCCGGAAGATGGCCGCTCAGAAGCAGCAGCAGATGCATCAGGAGCAGCTGAAGCAGCAGGTGGACAACAACCTGA *F ACTGCCAGGATCGGCTGATCGAGCACGTGACAGAGTCCTCGGCCGTTACCATCCAAAAGATGTGGCGAGGCTATCACACA *F CGCAAGAAGACCAACAAGGACATAGCCGAGCGATTGCAGCGACGACGCACACAGGAGTATATCGAACAACTCGGCAAGGA *F TATGCTACTTACAAAGGCCCAGCTCGAGAACGAGCGAAAGATTCAGCAGCTGCAGATGCAGGCCATCAATGCGCTGTGGA *F AAAAGGTGTCCACCATGGAGGTGGATCCCAAGGGAATACCTGCTGCTGCGGAACAGGGCGAGGACTCCAATGGCGGAGGG *F TCTGGCCACCTGAGTCTCGATCAAAATTCAGCCGCCGTGGTAAATGATTTGGCGAAAAGATGCACCATGCTCACCGATCA *F GGTTCAAATGCTGCAAAGCTCGATTGGAACCATCGTCAACTGCCTCACAATGGTGTGCAATCTGCCGCAGGACGCTATTA *F AGAAGCAGGCCGAGATCATTGATTGCAGCTCCACTCAGACGGACCTCATTGCCGTACACACGCCGCAAATCGAGGACCTC *F ACCAACTTTCCATTCACCAAAACAAGGCCCTCAACCTTGGCCCTAGAATCCAAGCACGAAGCTGCTCTGGCTTGTCCC # *U FBrf0188551 *a Murphy *b T. *t 2005.7.29 *T personal communication to FlyBase *u FlyBase error report for CG8188 on Fri Jul 29 13:58:41 2005. *F Date: Fri, 29 Jul 2005 13:58:41 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: tmurphy@ciwemb.edu *F Subject: FlyBase error report for CG8188 on Fri Jul 29 13:58:41 2005 *F Error report from Terence Murphy (tmurphy@ciwemb.edu) *F Gene or accession: CG8188 *F cDNA or EST error *F Comments: I have recently assembled full-length sequences for 3 cDNAs *F corresponding to CG8188, which indicate a more extensive 3' UTR than currently *F annotated. FASTA formatted sequences are appended below. These sequences *F have also been submitted to GenBank. *F >AT05212 *F TAAAAATCAAGATTTTCGAGAGGAGGTGCTATACCAAATACATATATATATATAGTCTTTAAACGAGTGC *F GTGTGAAAACATAAACCCAAACAACAGACAAGGAGACTAGCGGGGCCAAAGGAAGCATAAGGAGTCTACA *F CGCAAAGGAATAGTTAAAGCCAGCCCACAACGCACATATTCATATATTTTGGGACCACTTTTCCAAGGCA *F GCTGGGAAAAGGCGGTGAAATTGCGACTATCCGAGAGCCGAAGAGCTCAGCTGCAGGAATAAGCTGACGA *F AACTGAAACCTTGAGACAGCGATTCGCACGCACACCAGCGCAGGCCCGCATCAACACAGACACACACACG *F CACAGCAGCAGGAAATTCAGACATCATGAGTTCGCAATACTCGAATGTGGAGAACCTGTCGCCGCAGACG *F ATAAGGCAGGTGATGAGGGAGCTGCAGGAGATGGAAACCACGCCACCGGAAGGCATCAAGGTGCTAATCA *F ACGAGAGCGATGTGACGGATATTCAGGCATTGATCGATGGACCTGCTGGCACTCCGTACGCCGCTGGAAT *F TTTCCGCGTCAAACTGACGCTGAACAAGGACTTCCCGCTGACGCCACCCAAGGCGTACTTCCTCACCAAG *F ATCTTTCATCCGAATGTGGCCGCCAACGGGGAGATCTGTGTGAACACACTAAAGAAGGACTGGAAGCCGG *F ATCTGGGCATCAAGCACATTCTGCTCACCATCAAATGCCTGCTTATTGTCCCGAATCCGGAATCGGCGCT *F GAACGAGGAGGCCGGCAAGATGCTGTTGGAACGATACGATGACTACTCACAGAGGGCGCGCATGATGACA *F GAGATCCATGCCCAGCCTGCCAAATGCGGTGTAGGCGCTGTTGGCGATGCCAAAGACGATGGTGGACCCT *F CGACGAAGAAGCATGCGGGCCTGGACAAGAAGCTGCAGGACAAGAAGAAGGAGAAGTTGCTCAAGGAGAA *F GAAACGCATGCTGAAGAGATTATGAGAGGCATAAGGCGGAGTTCCATGGACTAGCTAATCAACCAGGAGC *F AGTAAGTAGTAAGCAGTAGCAGAACACGTCGGATACGTATGAATGGATAGATGTTAAAGAATGGATGAGT *F GCGAGCGTAAACCCTACAAACAGGTGAAAGGCGACAGGATCAAAGCTAGCAAGGTCGAATCAAAGGAGGA *F GGCGCGCTATGAAATTTTACACTGATCTGATAAAAAACAAAACAGACAAAAAAACAAGTAAATTGAGCTA *F CTGAAATTACGAAGTAACCGATGAAACCCGAACCCTGGAGGCCAATATCGGAACGGAACGGAAGTTAAAG *F CTGCAACAATTTCGGTAAAACGAGAGAGATAAAGAACCACTCCAAAGAACAGTGCATCCAGTTACCATAT *F ACGATTATTTTAACCAACATTTTTGGTGGCACGCCGCTTTCAACTGAGCCTAGAATATAGAAAACAAAAA *F CCAACAAAAAGCAACCAAGAGAAATGGGGATT >HL01424 *F AACGCCAGAGTCGAGATAGGTCACACAGCAGCTACGACAAAGTTGAGCGAATCGATTTCAAAACAAAAGC *F AACCAACGAAACAAAATTTTAAGTCGCTAGAAAAACTAAAAATCAAGATTTTCGAGAGGAGGTGCTATAC *F CAAATACATATATATATATAGTCTTTAAACGAGTGCGTGTGAAAACATAAACCCAAACAACAGACAAGGA *F GACTAGCGGGGCCAAAGGAAGCATAAGGAGTCTACACGCAAAGGAATAGTTAAAGCCAGCCCACAACGCA *F CATATTCATATATTTTGGGACCACTTTTCCAAGGCAGCTGGGAAAAGGCGGTGAAATTGCGACTATCCGA *F GAGCCGAAGAGCTCAGCTGCAGGAATAAGCTGACGAAACTGAAACCTTGAGACAGCGATTCGCACGCACA *F CCAGCGCAGGCCCGCATCAACACAGACACACACACGCACAGCAGCAGGAAATTCAGACATCATGAGTTCG *F CAATACTCGAATGTGGAGAACCTGTCGCCGCAGACGATAAGGCAGGTGATGAGGGAGCTGCAGGAGATGG *F AAACCACGCCACCGGAAGGCATCAAGGTGCTAATCAATGAGAGCGATGTGACGGATATTCAGGCATTGAT *F CGATGGACCTGCTGGCACTCCGTACGCCGCTGGAATTTTCCGCGTCAAACTGACGCTGAACAAGGACTTC *F CCGCTGACGCCACCCAAGGCGTACTTCCTCACCAAGATCTTTCATCCGAATGTGGCCGCCAACGGGGAGA *F TCTGTGTGAACACACTAAAGAAGGACTGGAAGCCGGATCTGGGCATCAAGCACATTCTGCTCACCATCAA *F ATGCCTGCTTATTGTCCCGAATCCGGAATCGGCGCTGAACGAGGAGGCCGGCAAGATGCTGTTGGAACGA *F TACGATGACTACTCACAGAGGGCGCGCATGATGACAGAGATCCATGCCCAGCCTGCCAAATGCGGTGTAG *F GCGCTGTTGGCGATGCCAAAGACGATGGTGGACCCTCGACGAAGAAGCATGCGGGCCTGGACAAGAAGCT *F GCAGGACAAGAAGAAGGAGAAGTTGCTCAAGGAGAAGAAACGCATGCTGAAGAGATTATGAGAGGCATAA *F GGCGGAGTTCCATGGACTAGCTAATCAACCAGGAGCAGTAAGTAGTAAGCAGTAGCAGAACACGTCGGAT *F ACGTATGAATGGATAGATGTTAAAGAATGGATGAGTGCGAGCGTAAACCCTACAAACAGGTGAAAGGCGA *F CAGGATCAAAGCTAGCAAGGTCGAATCAAAGGAGGAGGCGCGCTATGAAATTTTACACTGATCTGATAAA *F AAACAAAACAGACAAAAAAACAAGTAAATTGAGCTACTGAAATTACGAAGTAACCGATGAAACCCGAACC *F CTGGAGGCCAATATCGGAACGGAACGGAAGTTAAAGCTGCAACAATTTCGGTAAAACGAGAGAGATAAAG *F AACCACTCCAAAGAACAGTGCATCCAGTTACCATATACGATTATTTTAACCAACATTTTTGGTGGCACGC *F CGCTTTCAACTGAGCCTAGAATATAGAAAACAAAAACCAACATAAAGCAACCAAGAGAAATGGGGATTGC *F AACCATCACCCAATTAATTTATGTAGTTCTCTTTTCTTAACGCTCATGGGCTATCCTAGCATAATTTATG *F TAATTAGATTAAACAAAACAAGGATAACATTATCGCCTCATACATGCCACCTCTTAAACACCCAGAACTC *F TCTAGAAAGCATCACCTTACACATACCTTAATGAGCACTATAATCGCTCATTTGGGGTGAGTCCGCGTAT *F TGGACCCCCGTTCATAAACATAAACTTACCCCTTAGATTTCTTTTTTTTTTTTGTGTGTTTTTAAAATTG *F TATTCGCTTAGTTCATAAATAAATAAAATTTCGATTCGTTACATTCAATTGTCCTGCTTAGGCATAAGAT *F ACACAAAGGACACACACACCACCCATGCATCCAACCCACACAAATCACGAAAAATACATAAAATTATTAA *F AGAAAAAAAAAAAAAAAAAAA >RE30828 *F GGGTTACTGTAAAATGAAACTGTTTTTGTGTGTTGTTGTCGAGCGTGAAGTGTCCAATTAGACAACTGAA *F TTTGTTTAGATGTGAACGCCAGAGTCGAGATAGGTCACACAGCAGCTACGACAAAGTTGAGCGAATCGAT *F TTCAAAACAAAAGCAACCAACGAAACAAAATTTTAAGTCGCTAGAAAAACTAAAAATCAAGATTTTCGAG *F AGGAGGTGCTATACCAAATACATATATATATATAGTCTTTAAACGAGTGCGTGTGAAAACATAAACCCAA *F ACAACAGACAAGGAGACTAGCGGGGCCAAAGGAAGCATAAGGAGTCTACACGCAAAGGAATAGTTAAAGC *F CAGCCCACAACGCACATATTCATATATTTTGGGACCACTTTTCCAAGGCAGCTGGGAAAAGGCGGTGAAA *F TTGCGACTATCCGAGAGCCGAAGAGCTCAGCTGCAGGAATAAGCTGACGAAACTGAAACCTTGAGACAGC *F GATTCGCACGCACACCAGCGCAGGCCCGCATCAACACAGACACACACACGCACAGCAGCAGGAAATTCAG *F ACATCATGAGTTCGCAATACTCGAATGTGGAGAACCTGTCGCCGCAGACGATAAGGCAGGTGATGAGGGA *F GCTGCAGGAGATGGAAACCACGCCACCGGAAGGCATCAAGGTGCTAATCAATGAGAGCGATGTGACGGAT *F ATTCAGGCATTGATCGATGGACCTGCTGGCACTCCGTACGCCGCTGGAATTTTCCGCGTCAAACTGACGC *F TGAACAAGGACTTCCCGCTGACGCCACCCAAGGCGTACTTCCTCACCAAGATCTTTCATCCGAATGTGGC *F CGCCAACGGGGAGATCTGTGTGAAACACTAAAGAAGGACTGGAAGCCGGATCTGGGCATCAAGCACATTC *F TGCTCACCATCAAATGCCTGCTTATTGTCCCGAATCCGGAATCGGCGCTGAACGAGGAGGCCGGCAAGAT *F GCTGTTGGAACGATACGATGACTACTCACAGAGGGCGCGCATGATGACAGAGATCCATGCCCAGCCTGCC *F AAATGCGGTGTAGGCGCTGTTGGCGATGCCAAAGACGATGGTGGACCCTCGACGAAGAAGCATGCGGGCC *F TGGACAAGAAGCTGCAGGACAAGAAGAAGGAGAAGTTGCTCAAGGAGAAGAAACGCATGCTGAAGAGATT *F ATGAGAGGCATAAGGCGGAGTTCCATGGACTAGCTAATCAACCAGGAGCAGTAAGTAGTAAGCAGTAGCA *F GAACACGTCGGATACGTATGAATGGATAGATGTTAAAGAATGGATGAGTGCGAGCGTAAACCCTACAAAC *F AGGTGAAAGGCGACAGGATCAAAGCTAGCAAGGTCGAATCAAAGGAGGAGGCGCGCTATGAAATTTTACA *F CTGATCTGATAAAAAACAAAACAGACAAAAAAACAAGTAAATTGAGCTACTGAAATTACGAAGTAACCGA *F TGAAACCCGAACCCTGGAGGCCAATATCGGAACGGAACGGAAGTTAAAGCTGCAACAATTTCGGTAAAAC *F GAGAGAGATAAAGAACCACTCCAAAGAACAGTGCATCCAGTTACCATATACGATTATTTTAACCAACATT *F TTTGGTGGCACGCCGCTTTCAACTGAGCCTAGAATATAGAAAACAAAAACCAACATAAAGCAACCAAGAG *F AAATGGGGATTGCAACCATCACCCAATTAATTTATGTAGTTCTCTTTTCTTAACGCTCATGGGCTATCCT *F AGCATAATTTATGTAATTAGATTAAACAAAACAAGGATAACATTATCGCCTCATACATGCCACCTCTTAA *F ACACCCAGAACTCTCTAGAAAGCATCACCTTACACATACCTTAATGAGCACTATAATCGCTCATTTGGGG *F TGAGTCCGCGTATTGGACCCCCGTTCATAAACATAAACTTACCCCTTAGATTTCTTTTTTTTTTTGTGTG *F TTTTTAAAATTGTATTCGCTTAGTTCATAAATAAATAAAATTTCGATTCGTTACATTCAATTGTCCTGCT *F TAGGCATAAGATACACAAAGGACACACACACCACCCATGCATCCAACCCACACAAATCACGAAAAATACA *F TAAAATTATTAAAGAAATACTGTAAAAGATTGATTGCACATTCTGTAGAGATTTAGTTTTAAGTGCTTCC *F AAATTGTCAAACCCTCGACGCGAATCGGATCGGAATGGAATGGCCTGGAGCGGATTGGAAAGCAATGGCA *F CGGTGATAAGTGCATGGGATATCCGTATCCTCGTCCTTTCGCTTGCTATGTCGCCGGTATTCTACACGCC *F CGCTGCTCTCTCGCTCTCTCTCCCTCCTTTCGTTCGAACATCCTTTTGAAGCTCACACAACAAACACACA *F CAAAACGAGGGCAAAGAGTAAAAAGCAATAAAAAAGAATAAAATTCCAGAAAATTACAAAAAAAAAAAAA *F AAA # *U FBrf0188552 *a Imler *b J.L. *t 2005.7.25 *T personal communication to FlyBase *u FlyBase error report for vir-1 on Mon Jul 25 15:18:03 2005. *F Date: Mon, 25 Jul 2005 15:18:03 \-0500 (EST) *F From: FlyBase-error@rail.bio.indiana.edu *F To: flybase-updates@morgan.harvard.edu *F Cc: JL.Imler@ibmc.u-strasbg.fr *F Subject: FlyBase error report for vir-1 on Mon Jul 25 15:18:03 2005 *F Error report from Jean-Luc Imler (JL.Imler@ibmc.u-strasbg.fr) *F Gene or accession: vir-1 *F Missed gene *F Comments: We have identified a previously unrecognized transcript of CG31764. *F The transcript is strongly induced by virus infection in adult flies, and was *F named vir-1 (virus-induced RNA 1). The cDNA was cloned by RACE-PCR and *F sequenced, and the transcription start-site was determined by S1 nuclease *F mapping. The work is described in a paper in press in Nature Immunology. The *F sequence of the vir-1 cDNA is: *F TCAGTCAAAAAAGTGATCAGTACGCGTAAAGAGGTGCCATCATTACTCCGAATTCGAAGCTTCCGATATCAGTGAGGATA *F TAGTTGAATCTCAACCGGAAGAGCAAAAGAACACCATGGATCAGCTGCAGCAAATGATGGAACCCCTGCGAGAGGAGATC *F AGACATGAGGAAGATTTGGAGCAGGAACACGAGGACAAGGAGTACTTGGAGAGGAAAGAGAAGAAGGAGCGTGAAGAATT *F CGATTCCTCAGACGATGAAGACGGCGAGGCTACAACTCCTGTTGTGATGAGCGAAACCTTCAATGATGAGTGGGCCGAAT *F TCGATCAGGATCAGGATCGAGAGCACGATCGGAATGAGGATCAGGATCATGATTTGGACTTTGAAAACGATCTAAACAAC *F GAAATCGATATCGAGGAGAACTTTGTGCCCATTGACCTATCCAACGATATAGCCGTCAACGATTTGGCTGCCGCAGATCC *F CAATTTTCCCATCAATGAAGATGCCGAGTTCATTGTGCACCCAGCTGTAATCAGAACTATGCCTATGTTCGAGAAGCTTT *F CTTTAGGAGAGCCTGCGAAATGAGGTTTCTAAGTCTCTCCCACTAGTCTAGGCATTTTCTTTTAGTCTTAACTCAATAGC *F TTGTAAACATAAAACGAGGATATCTCTGAGAGATCCAAAGAAATCGGCGCTCATTTCATCCGTAATTTATCCTATTTATT *F TTCACAGACAACGAGTATTATGTATGCAAGGTGACATTGAAAATTATTTATTGTTAAAATAAAAGAAATCAAAAATAAGT *F GTATTAATATAAATAAGTCTTCGATAATTGGCTTAATTTATAATGAAGTGCAATAAAATATATGCGATGTCTTTGATAAA *F ACTGACTATTATAG # *U FBrf0188553 *a Tully *b T. *t 2005.7.29 *T personal communication to FlyBase *u *F Date: Fri, 29 Jul 2005 14:51:28 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 076 Gene data (problem or question) *F .. *F Hi, *F Tungus was mentioned as supplemental material in the Dubnau et al (2003) *F paper. The tungus mutation is produced by a P element insertion, which is *F listed in the genomic sequence below (look for the 'P'). In the original *F release of Flybase, this sequence was listed as part of CG8242. With the new *F release of FlyBase, however, it appears as though our P-element insertion now *F lies within nearby CG8253. *F Below is the DNA flanking sequence surrounding our P insertion (red 'P') *F from line A0469 (tungus). *F GGTAGCCTACATATCCTGGCCATGAGCTGCGCTGCCAGCGTGTTAATATTCAGGT *F GCTGAAGGCTGGGTGAATGATTTCGGCTGCCTAATGAAAAAGTTTTCCATCTCCG *F TGCGGTGCTCTTGTTCTTGTTGTTGTTTTCGTTTGTCGTTGTCTGTTGCTTTTGTTC *F CTGCCCATGGACATGGGCATGGGTATATATGTATATTCGCATGAATGGCCATATA *F ATCACCAGTAACAGGACACGTAGGAGCAGTCCGATATTTTAGGGAAGAGGAAGT *F CCGTGGTCATCTTGATGCGCTCCGGGATCTGCGATGCTGCGATGCTGGTCGCCTG *F TGTTGCTATTGTGGCTGTTGCTGTGGGTGCGGGTATCCTTGAGCCAGGACCTGTG *F GGTTGATTTGCCGTGGAGGATATGGATATGGAGACGGAGATGGAGCTAATTGTC *F ACTGGGGACTGGGAGATTGCACGAGACACGAGAGGAGCTGGTTTATATTATCAC *F TGCATTATGCGCCGCTGCCTGGACAAAAGCAGGGTGTGTGTATCCGTATCTGTAA *F TCTGTATCTATGTCTATGCGGTGGCATCGGAGGATGCTGGACGCTGGCTAATCCT *F TATTCCGATCCTGCTCGTCGCACTGGCACCTGCTCAGCGACATTCCTCCATTCCC *F AGGGAGGCTGCGATGCGGATGATGGCCCCTCTGCTATGTTTTTATATTTTTGTTA *F TTTCTCCTGTCTTTTTCTGGCTTCCAGGATGCTGCTCACGCACACACTGGCACACG *F GGCACGCACACACCGCCACATACACATACACATAPCACTGGGGGCTCTCTCTCG *F CAAAGGAGCCGATTTCTCGCTTTCGTTTCGTTTGGTTTCGTTTCGATTTTTGCGAA *F TTAAATTATGCTAAGGAGCCAAGGAGCTAAGGATGCTGGAGCTTTTATCCCAGCC *F TGCTGCCTTGCAGCTCCTGTTCACTCGTTACGGGATCGCACACCTGACCAAAAAC *F AAACGCGCTCATCCCCCGCAGATTTTTCTGTTGCACTGCAGCGGGCGAGGATGGG *F GCGGCCTGACCAGGTGACTGAGCCTGGGGCAACACGCACTCAGCGACCCGTGGA *F GCTTGTATCACGGCGGAACACTACGTTGGTCTGGGCACCGGAATCCTTGGATGTT *F GCAGGCTGCGTGCGTTTTTCGTGGGCGCCTCTATCAGCTTTTCGTTTTTTTCCCTT *F CCACGCTCTGGTGGTGGCTTTTCCGCTTCTTTTTCTTTCTTTTTTTCTTTTGCTCGG *F CACACTCCGCTCCTTGTGTTGTGTACGCGTTCCCGAGTTCGCACAGTGTGACCCA *F AGTTGGCGAGCGCCACAATTCCAGAACAAGTTCTGTGTTTGTTGTGAAAAATGAA *F CAAGTTCTGGTTTCCACTGAGCTGAAAGTAAAAGCGCGCGCGATTTGAAAAGCAT *F AAGAAACAGTTTGAAGATATAATAAATGTAATAAATACAAGAAACAATAAAAGT *F ACAAATATAAACTAATTGGCCACTTAAAAAATAAAGTTTTAATGAATTTAAAGAA *F ATATATATATAAAATAAATATTATAAATGGCTAAGCTATTTTATTCTTCTTAAAA *F AGGAAATGTATAATTTTTTTTAATAAATAAAAGTATTTTCTTAACCAATTTTAATA *F CATGAAAATTCCAAACTTATATTTTCCAATAAGCATTTATTCTTATCGGAAATTAA *F TCAATCAGAATCCAATTGAGCGCAGCAAGCAAACAGTGATGCCCATCGCATACG *F CACAAAAGCTATTAGCTATGTTGCACGCTGTGAGCTGCTCACTGCTGCGACGCTC *F CAAGCTAGCTGACAGCAGGTAATTGCTACAGTCAACGGTTTTTCGAAACACATTT *F TGGATGCCACTAGCAAATATTTGTTGCTAACACCTCCGTTAATAATCGCTATCTA *F ATCACCCGTGATTGCTAATGCCCAATTGATGGCACGCAATTTGGCAAAGCCAATT *F AGTATTGTCTCATTTTTACGGCACTCGACAAGACGACGGCAGGCAAGACGAAAGA *F CCTTTCGTTCGATAAGATAAATCTGAAATCTGCGCAGTCCGTTGTTGTGCCATTG *F TAAATGCCTCTTGAGAACATTTCCTACACACCCAGGCGACGAACGCACCGCGAGT *F TAATCGTCGAAACTGATAAGCTGATATAATAACTATACTATATATATGACAGGTT *F GACTTAACCCACCCAGATGGGCCCACAAATGAACCATCGCAAGCGTATGGGCTG *F TTAAATGGTTACTTAATGTTTCGTGTCCAACAAGCCGATAAGGACAAAGTGCATC *F GGCATATTGTCAACCCTATGCCCATTCAGAAGTCCAAACCGATCAGCAGTATCGC *F CCATTATCAGCGATCAAAGCGATCATGACACACATAAAGGCAAATCTATTGGCC *F GGATGACGTTCCTAGATGGC *F and below is our original, specific plasmid rescue sequence from *F A0469:TTTNANTCCATTAGCANTTCNTGNATCATGCACTGGGGGCTCTNTNTNGNAAAGGAGCCGATTNCTCGCTTTC *F GTTTCGTTTGGTTTNGTTTCGATTTTTGCGAATTAAATTATGCTAAGGAGCCAAGGAGCTNAGGATGCTGGAGCTTTTA *F TCCCANCCTGCTGNCTTGCAGCTCCTGTTCACTCGNTNCGGGATCGCACACCTGACCAAAAACAAACNCGCTCATCCCC *F CGCAGATTTTTNTGTTGCACTGCANNGGNCGAGGATGGGGCGGCCTGACCAGGTGACTGANCCTGGGGCAACACNCACT *F CANCGACCCGNGGAGCTTGTATCACGGCGGAACACTACNTTGGTCTGGGCACCGGAATCCTTGGATGTTGCAGACTGCN *F TGCGTTTNTCGTGGGCGCCTCTATCAGCTTTTCGTTTTTTTCCCTTCCACGCTCTGGTGGGGGCTTNNCCGCTCCNTTT *F TCTTTCTTTTTTTTTCTTTTGCTCGGCACACTCCNCTCCGTGTGTTGTGTACGCGTTCCCGAGTTCGCACAAGTGTGAC *F CCACGTTNGCNAGCGCCACAATTCCANAACAAGTTCTGTGTTTGNTGNGAAAAATNAAACAANNTCTTGGTTTCCACTT *F GAAAACGAAAAGTAAAAGCGCGCCGCCAATTTG *F so you can blast this yourself to confirm. *F .. *F   *F Tel 44-(0)115-924-9924 *F ext. 43919 *F Fax 44-(0)115-970-9906 *F realname: tim tully *F reply-to: tully@cshl.org *F source: FB Help Mail: # *U FBrf0188554 *a Heriche *b J.K. *t 2005.7.14 *T personal communication to FlyBase *u *F Date: Thu, 14 Jul 2005 05:42:15 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 042 Gene data (problem or question) *F comments: Hi, *F As part of the TreeFam curation process, I looked for a fly *F ortholog of APC subunit 13. One is described in PMID: 15060174. *F However, its sequence appears to fall in exon 2 of CG6355 in a *F different reading frame as that of CG6355 protein. In addition, the *F N-terminal of CG6355 protein aligns poorly to orthologs in other *F species. Is it possible that fly APC13 has been mistakenly included *F in the 5' end of the downstream gene ? Or if the evidence for the *F CG6355 structure is strong, is it possible to have another gene *F nested in its 5' end ? *F Thanks *F Jean-Karim *F realname: Jean-Karim Heriche *F reply-to: jkh1@sanger.ac.uk *F source: FB Help Mail: *F Date: Fri, 15 Jul 2005 14:38:39 \-0400 (EDT) *F From: Madeline Crosby *F Subject: Re: FB Help Mail: 042 Gene data (problem or question) *F To: flybase-help@morgan.harvard.edu, jkh1@sanger.ac.uk *F Cc: crosby@morgan.harvard.edu *F Dear Jean-Karim, *F > As part of the TreeFam curation process, I looked for a fly ortholog *F >of APC subunit 13. One is described in PMID: 15060174. However, its sequence *F >appears to fall in exon 2 of CG6355 in a different reading frame as that of *F >CG6355 protein. In addition, the N-terminal of CG6355 protein aligns poorly *F >to orthologs in other species. Is it possible that fly APC13 has been *F >mistakenly included in the 5' end of the downstream gene ? Or if the evidence *F >for the CG6355 structure is strong, is it possible to have another gene *F >nested in its 5' end ? *F I think this must be a new gene for FlyBase. It looks like the second *F situation you describe is the case: it is encoded within the 5' UTR of CG6355, *F which means this transcript is dicistronic. Dicistronics are not particularly *F rare, there are approximately 50 such pairs annotated in the genome at the *F present time. *F Thanks very much for bringing this to our attention. Anapc13 is so small, we *F would not have noticed this error by simple inspection of the annotation. *F Sincerely, *F Lynn Crosby *F FlyBase *F > Jean-Karim *F > realname: Jean-Karim Heriche *F > reply-to: jkh1@sanger.ac.uk *F > source: FB Help Mail: *F > # *U FBrf0188556 *a McNabb *b S. *t 2005.7.29 *T personal communication to FlyBase *u *F Date: Fri, 29 Jul 2005 15:34:27 \-0700 *F Subject: bursicon mutant mistake *F From: Susan McNabb *F To: Beverley Matthews , Hans-Willi Honegger *F *F Dear Beverley, *F I reassessed our data on bursZ1140 in response to your query (below) to *F Dr. Honegger, as my group was responsible for characterization of the burs *F mutants. I found that both the location of the mutation and the nature of *F the amino acid change in the paper are incorrect. The correct location is at *F residue 63 (not 97). The mutation changes a tyrosine to a cysteine (not *F threonine to cysteine). The mutation is a single base change in which the *F TAT codon is mutated to TGT. *F To clarify, the sequence around the mutated site is VLQYPGC. The Y is *F mutated to C. *F I apologize for this error. Thanks for bringing this to our attention. *F Yours truly, *F Susan McNabb *F \-- *F Susan L. McNabb, Research Asst Prof *F Department of Biology, Box 351800 *F University of Washington *F Seattle, WA 98195-1800 *F tel: (206)543-6513 *F fax: (206)616-2011 *F email: smcnabb@u.washington.edu *F Dear Dr. Honegger, *F I am a curator for FlyBase and am currently capturing the genomic sites *F of the burs mutations that you reported in your 2004 Current Biology *F paper, to appear in the genome maps in FlyBase. I have a question about *F the bursZ1140 allele. The text says that each of the five reported *F mutations has a single base change in CG13419, but bursZ1140 is *F reported as a change from Thr to Cys, which would require conversion of *F two of the bases of the ACC (Thr) codon to get a TGC (Cys) codon. Can *F you please let me know what the actual base changes that you observed *F are? *F Thank you for your help, *F Sincerely, *F Beverley Matthews *F Curator, FlyBase-Harvard *F bmatthew@morgan.harvard.edu # *U FBrf0188558 *a Robertson *b H. *t 2005.7.22 *T personal communication to FlyBase *u Dpse Ors and Grs. *F Date: Wed, 20 Jul 2005 00:11:39 \-0500 *F From: Hugh Robertson *F To: Peili Zhang *F Cc: bill@morgan.harvard.edu, flybase-help@morgan.harvard.edu *F Subject: Re: Dpse Ors and Grs *F Dear Peili and FlyBase, *F I've completed a first pass at trying to reconcile all of my versions of *F the DpOrs with yours. Below are comments on every gene. I am also *F attaching a draft of the Or table from our manuscript if that helps at *F all. I hope to have the Grs done by the end of the week. I don't imagine *F these changes can make it into Release 2, but perhaps there will be a *F Release 3? Any chance I could get GA numbers for those that don't *F currently have them to include in the manuscript? *F Hugh *F Or1a \- GA14708 \- Fine as is. *F Or2a \- GA16647 \- Needs nine aa on the N-terminus. *F MQPGSENQPELNTHSAVDYHWRVWQLTGLIRPAGISKSLYRTYAIGLNLMVTLLFPLSLLARLILTRSMKELCENLTITI *F TDITANLKFGNVYLVRRHLGEIRSLIQQLDCRALAICDRDELCALVQAVTTARNTFRTFAWIFVCGTSLSCVRVALARSR *F QLLYPAWFGVDWKNSNEAFVGIYVYQLFGLIVQAVQNCASDSYPPAYLCLLTGHMRALEVRVARIGCECASGQQSYDQLL *F ACIQDLTLIHRMHTIIQQILSVPCMAQFACSVAVQCTVAMHFLYVADADDRLAMILSVIFFVAVTLEVFVICYFGEKMRT *F QSEALCNAFYACNWVEQLPRFRRDLLFTLARAQRPSLILAGSYIPLTLETFKEVMHFAYSAFTLLLRAK *F Or7aP \- No GA# \- A fragmentary pseudogene in Dp \- here's chunks of *F translated pieces *F APEDSRPARTPLWRRIYRCFAAMIYVWQLXGIWGGMEITQVMTRKDLGSDLGSAAAATALDGQSRETSEFRRIGKAVRFC *F NRLVWFYQLLLFYLHQAQQPIALTAMKIFPINLAIAKFSFSLYALIKAMTLGERE *F Or9a \- GA13635 \- First two and last aa missing. *F MAKKDETNSLRVQILVYRLMGIDLWSPTAVNDRHLVTFVTMGPLFAFMLPMFLSARENITEVSLLSDTLGSTFASMLTLV *F KYLLFVYYRKEFVGMIYRIRSILEKEINVWPEAKEIVDAENRSDQMLSLTYTRCFCMAGVFAAIKPFVTMSLALLRDGGD *F GSKLHLELPHMGVYPYDYQVMWFFVPTYLWNVMASYSAVTMALCVDTLLFFFTYNVCAIFKIARQRLVHLPAMGEADPRG *F ELEAAVQVLLLHQKGLWIADFIADHYRPLIFLQFFLSALQICFIGFQVADLFPKPQSLYFIAFVGSLLIALFIYSRCGEN *F IKEASLDFGDGIYESNWMEFAPSTKRVLLIASMRAQRPCQMKGYFFEASMATFSTIVRSAMSYIMMLRSFNV *F Or10a \- GA14703 \- Needs five aa on N-terminus. *F MWSFHRLLRRDQPLRSYFFAVPRLSLDVMGYWPMGDDLPVRAIVHFVILSIGVVTELHAGFMFLQNAQITLALETLCPAG *F TSAVTLLKMLLMLRYRRDLTNVWRQLQRMLFDAGLNRPEQKAIIHDNSVLAARINFWPLSAGFFTCTTYNLKPLLIAFIL *F YLQDPDQELPWNTPFNMTMPKVLLAAPFFPLTYAFIAYTGYVTIFMFGGCDGFYFEFCVHISSLFQSLQEETRAIFRPFE *F EYLKLTPAQCARLELQLRGLIIRQNSVFELISFFRKRYTVITLAHFVSAALVIGFSICNLLTVGNNGLGALLYVAYTVAA *F LSQLLVYCYGGTLVAESSFELSRVAASCPWSLGAPRQRRVILLLILRSQRAPTMAVPFFSPSLNTFASILQTSGSIIALA *F KSFQ *F GA17050 is the same gene, but with the N-terminus intact, an open intron *F unspliced, and the C-terminus spliced into the Gr10a ortholog *F immediately downstream. As in the Gr notes, I recommend removing the *F Or10a coding region from the front of this annotation and making it just *F the Gr10 ortholog. *F Or13a \- Not in BLASTP or in list \- A single long ORF \- unclear why not *F annotated. *F MFNLQQYTAITFRLPVQCVWLKLNGSWPFVPSSRKDNSSMCSILYTAWAWYVIVSVGITIGFQTAYLVTHLSDIIMTTEN *F CCTTFMGALNFVRLLHLRFNQRKFRQLIEQFERDIWIPENTHISVNAECHKRMFTFTIMTGLLSCLICMYCALPLVEIFF *F RTGVDAVEKPFPYKMLFPYDPYSSWMRYVFTYMFTSYAGICVVTTLFAEDSIFGFFITYTCGQFRLLHERVDNVLTAAKE *F RADEQHLQLQRLHNIVQQHNKIIRFAKCLEDFFNPILLVNLMISSLLICMVGFQIITGKNMFIGEYVKFIVYISSAISQL *F YVLCENGDALIIHSTNTARHLYGCDWENPDIRNFYSYTSMSHQLRNDLKFMILCSQRPVRITAFKFSTLSLQSFTAILST *F SMSYFTLLRSLYYEDQL *F Or19a \- GA17168 \- This protein can be extended 18 aa at N-terminus, *F although the Dm ortholog does not extend like this. I'm not sure what *F the best approach is for examples like this, of which there are several *F in Dp. *F MGYKRVWPSRGQSKVLDDMRPVDSMSAFRYHWQIWRVMGMHPADPQTLWGRHYTLYGIVWNAFFRLGMALSLVVNFLLST *F SLESFCESLSVAVPHTVANLKVFFLWRMRQQILQTHPILHHLDGRIGSLAEKQSILEGIDRAYFTFISFLRAIIFILAVG *F ILILCLSSDRPLLYPSWMPWNYKDSSFTVYAMTVCLHSVGIIENALLVCNVDTYPGSYLNMLAAHTQALAHRVSRLGYDP *F RLTRSQACDRLRSCILDHQIIMNLFKSLEHSLSMSCFLQFASTAIAQCATCFFVIFVSVGTMQSVNMIFLFLVFTTQTLL *F LCSSAELVRHEGENLIKAIYDCNWLDQSVEFRRMLLLMLARSQRPMILRAGLIIPVQMSTFMVVCKGAYTMLTLLREVDN *F SEVA *F GA15104 is listed in your table as an ortholog of DmOr19a, and is shown *F in the genome browser in the same location, but it doesn't come up in *F the BLASt searches. Dm Or19a/b are very recent duplicates of a single *F ortholog in Dp, GA17168, so perhaps GA15104 should be dropped? *F Or22a/b \- This duplication is independent of a triplication in Dp *F yielding two intact genes for which I propose the models below for the *F GA numbers shown in the genome browser. *F Or22a1 \- GA11469 \- not in BLASTP \- here's my model *F MLSKLFPRIKAKPLTERIQSRDAFVYLDRVQKLWGWRATEDERWMVLYNIWAFIWNVLLLVLLPLSMSMEYVQRFKNFSP *F GEFFGSLEICVDMYGCSLKCVYTMFGYKRFQAARKLLDRLDLRCTSDEDRASVHRSVALANRCYVTYHILYSGFVVINWT *F GYLLLGSHAWRMYLPGLDSEKNFLVTSFFELLLMSGVVTMNQCTDVSPLAHMIMARCHMGLLKDRLNKLHSDPSKTEEEH *F QEDLNRCIHDHCVILDYVNLLRPVYSVTIFVQFLLIGLVLGLSMIHIMFFSNFWTGIGTMCFIFDVCLETFPFCYLCNII *F IEDCRELSESLFQSDWLGASRKYKSTLVYFLHNLQQPIVLTAGGVFPICMQTNLSMVKLAFSVVTVIKQFNLAEKFQ *F Or22a2 \- GA18049 \- not in BLASTP \- here's my model *F MLSKLVPRIKAKPLTERTSSRDAFVYLDRVQKFFGWTAVEDKRWRIPYILWGIFMNLLLIFFLPISMLVAYIQMFKSFTA *F GEFLSSLEITVNMYGCVLKCIYTIWGFKGFTAARKVLDELDLRCTSDEERTSVHRCVALGNLSYVLFHIFYSGFVVINWT *F GYVLMGRHAWMMYLPGLDAENNFFVASLCEILLMSGVVTMDQCTDVSPLAHMLMARCHICLLKDRLTKLRTDPTKDEDEH *F YEELSNCVHDHRLILDYVKALRPTFSGTIFVQFLLIGIVLGLSMINVMFFSTLWTGLGTVCFMFCVCLETFPFCYLCNMI *F IDDCQKLSDNLFQSDWTTASRRYKSTLVYFLQNLQKPIILTAGGVFPICMQTNLSMVKLAFSVVTVIKQFNLADKFQ *F Or22aP \- Then there is a pseudogene most similar to GA11469 immediately *F upstream of GA18049 *F RKSKLVPRITAKPXDVLVYLDRVKKLWGWRAAEDEQWRILYNIWAFVWNMLLLVLLPLSMSMEYVQRFKXFSPGEFFGSL *F EICVDMXAKTLLDGLDLRCTSDEERASVHRYVAPANXCYVAYHILYSGFVVINWTGYLLLGRHAWRMYLPGLDSEWNFLV *F TSFFELLLMTAVVTMNLARCHMGLFKDRFTKLHSDPSKSEEEHQEDLNRYIQDHSEILEYVHLLRPVYSSTIFVQFLLIG *F LVLGLSMIHIMFFSNFWTGIGTMCFMFDVCLETFPFCYLCNIITDHCQDLADSLFQSNWMAASRRYKSTLVYFLHNLQQP *F IVLTAGGIFPICMQTNLSMAKMAFSVVTIIKQFNLADKFQ *F Or22c \- GA 13684 \- fine as is *F Or23a \- GA 22094 \- fine as is *F Or24a \- GA 11185 \- fine as is *F Or30a \- GA12048 \- Needs an internal segment restored. *F MDLKSMDTVDMPIFGSTLKLMKFWSYLFVHNWRRYVAMTPYIVINCTQYVDIYLSTESLDFIIRNVYLAVLFTNTVVRGV *F LLCVQRGSYERFIEVVKAYYIQLLESKDAHILRLVEEITRLSITIGRINLLMGTCTCIGFVTYPIFGSERVLPYGMYLPA *F IDEYKYATPYYEVFFVIQAIMAPMGCCMYIPYTNLVVTFTLFGILMCRVLQHKLRSLEKLEKGLVRREIIWCIQYHLKLA *F GLVDAMNSLNTHLHLVEFICFGAMLCVLLFSLIIAQTIAQTVIVIAYMVMIFANSFVLYSVANELYFQSFDIAIAAYESN *F WMDFDIDTQKTLKFLIMRSQKPLAILVGGTYPMNLKLLQSLLNVIYSFFTLLRRVYG *F Or33N \- lost from Dm \- This gene is upstream of the three that are *F orthologs of DmOr33a-c *F MELPSPVIASDYIYRTYWLYWRLLGVEGEHPLRYLLLIMQFFFVTIWYPIHLIVGLICDGTLAEVCRGIPITASCFFASF *F KIICFRWKLAEIKKVQQLFVELDQRIATAEERSSFYRETIRVAEFIGKSLLVAAFLAIITGTAFGLFRRERNLLYPGWFP *F YDVYSSDQRFWLSFSYQAAGHSLAILQNLANDSYPPMTFCVLAGHVRLLSMRLSRMGYDLTKPKELIVRELKDNIEDYRK *F LMKIVQLLRSTMHLSQLGQFISSGINIAITLVNILFFADNNFARTYYGVYFMAMLMEIFPSCYYGTLVSMELNGLTDSIF *F SSNWVGMDRGYCRTLLIFMQLTLAKVEIRAGGMIGISLNAFFATIRMAYSFFTLAMSLRK *F Or33a \- GA14239 \- Not in BLASTP \- My model is *F MASDPPDVRSGHIYRTYWLYWRLLGVEGEYPLRYLLDVILNFFVTIWYPTHLIIGLFQERTIGHVCKNLPFTAESFFCSF *F KIICFRWRLAEIKKIEQLLMELDQRAVSPEERVFFHQNTRSVAEFISKSYVAAGISATVTGTASVLFSSGRKLIYPAWFP *F YDVQASALRYWLSFTYQATGATLTILQNMANDSYPPMTFCVVAGHVRLLAMRLRRMGHHEKASKQGNAKKLIENIEDHRK *F LMQIVRLMHSTLYLSQLGQFISSGINISIVLINILFFAENGFAIIYYVVYFMAMVLELFPSCYYGTLMSMEFQKLPYAIF *F SSNWLGMGRRYCHTLLVLMQFTLTEVDIKAGGMVGISMNAFFATIRMAYSFFTLAMSFR *F Or33b \- GA14240 \- Fine as is. *F Or33c \- GA18589 \- Just needs three aa on N-terminus. *F MAAVIDSVRVYQPFWWCMRVMAPTFFGATQRPVQIYVGLLHLLVTFLFPVHLLVNLALQPTSAELFQNLSISMTCAACSL *F KHVAHLYHLQEIAEIQKLLIELDGYVDSEEEHRYYVDHLQCQARRFTRCLYASFVVIYVLFLLNLMILIASEDRMLVYPA *F YFPFDWQGNGYLYAIAVGYQSICLVLEGIQGVSNDTFSPLTLCFLGGHIHMWGLRMQKLGYEEEDEESPSVNHHQQLLNY *F IEQHKILMRLHRLTRHTVSLAQLVQLGCSGASLCIIVCYVLFFVRDIITLLYYVIFIAVICVQLFPACYFASVVAEEMQS *F FPYAIFSSKWYEESREHRRDLLIFTQLTLVGRSRVIKAGGLIELNLNAFFVTLKTAYSLFALVVQVKDI *F Or35a \- GA14704 \- Not in BLASTP \- My model is *F MVRYVPRLADGQRVRLAWPLALFRLNHIFWPLDPSTGKWGRYLDRFLAVLGCLIFVQHNDAELRYLRAEASNLNMDAFLT *F GMPTYLILVEAQFRSLHVLLHFEELQRFLQRFYTTIYIDPRAEPDMFRRVDGQMLINRLVSAMYGAVISGYLISPVLSVI *F NRRKDFLYSMVFPFDTEPLAVFVPLLLSNVWVGIVIDSMMFGETSLLCELIVHLNGRYLLLKRDLEESIQRILAERRRPQ *F MARQLKELIIATLRQNVALNQFGEQLEAQYTVRVFIMFAFAAGLLCALSFKAYTNPMANYIYAIWFGAKTVELLSLGQLG *F SSLAYTTDSLGSMYYHTHWEQVLEQSSNPLETLRLLRLIQLAIEMNSRPFYVTGLKYFRVSLQAGLKILQASFSYFTFLT *F SMQRRQMSN *F Or42a1 \- Not in BLASTP \- a recently duplicated copy of the gene below *F MVLRKIFPAMYTLSEEAPACSRNGTLYLMRCIFVMGVRKPPARFFVAYCLWSIVMNLSSTFYQPIAFLTGYISHLSELSA *F GELLTSLQVAFNAWSCSAKVLIVWALIKHFDDANDILDEMDRRLTQPSVRLRVHRAVSKSNRIFFIFMTVYMSYATNTCL *F TAIANGKPLYQNYYPYLDWRSSSLHLGLQTGLEYFAMAGACLQDVCVDCYPVNFVLVLRAHMSIFADRLRQLGSDPEESP *F EQRYEQLIQCIQDHKTILRFVDCLRPVISGTIFVQFLVVGLVLGLTLINIVLFANLGSAIAALFFMAAVLLETTPFCILC *F NYLTDDCYNLADALFESNWIDGEQRYKKTLMYFLQKLQQPIKFMAMNAFPISVGTNIVVTKFSFSVFTLVKQMNIAEKLA *F KVEGEADFN *F Or42a2 \- GA14414 \- Fine as is *F Or42b \- GA11791 \- Fine as is *F Or43a \- GA14981 \- Needs three aa on N-terminus. *F MATTIDDIGLVGINVRIWRYMAVLYPTPGTSWRKFAFVLPVCAMNLMQFFYLLRMWSDLPAFLLNLFFFAAIFNSLMRTW *F LVIIKRREFEKFLEELFRLYRWILDSGDEYSRTILLEAEREAHRLAVFNLTASFLDIVGALVFTLFKDERSHPFGVALPL *F LDMTRTPVYEIFYLLQIPTPLLLSVLYMPFVSVFAGFALFGRAMLRILVHKLSLIGGQQQDAGARYQRLTACIRFYIEVL *F GYVRNLNNLVNLIVAIEAIVFGSIICSLLFCLNIITSPTQIVSIVMYILTMLYVLYTYYNRANDLVIENALVADAVYNVP *F WYEGNMRFRKTLLIFLMQTQCPLEIRVGNVYPMTLAMFQSLLNASYSYFTMLRGVTNK *F Or43b \- GA14700 \- Needs a couple aa on each end *F MFFKLVYPAPLSEPIGTRDSTVYLLKTLHIAGLDFYNDFGIGRKILRVISFSYNIFYLPLSFPINYKIHFSQFPPDLLLQ *F SLQLCLNTWCFSIKFFTLSILKERFEMANKCFDELDVYCVTPEEKRKVRVTVATINKLYLIFGIVYFLYATSTLVDGLFH *F DRVPYNTYYPFIDWRLDRRQLYIQSFVEYFTVGYAIFVATATDSYPVIYVAALRTHILMLKDRIVRLGEANNEANADPDN *F IFKSLVECIKAHRTMLNFCDTIRPIISGTIFAQFIICGSILGIVMINMVLFADQSTRFGIVTYVMAVLLQTFPLCFYCNA *F IVDDCNDLADSLFHSAWWMQDKRYQSTALQFLQKLQQPITFTAMNIFTINLATNINVAKFAFTVYAIASGMNLDEKLQLQ *F DSGADNP *F Or45a \- GA15169 \- Needs a C-terminal exon. *F MDDSYFSIQRRALEIVGFDPSTQRLHMRRPLWAGLLILSLVSHNWPMIVYGLQDLSDLTRLTDNLAVFMQGSLCTLKFL *F AFIVKRRRIGALVHRLHGLNQEACASPLQREKILRENRLDMYVSRAFRNAAYAVTVASMIAPMLNGLIAYLTEGVFRPT *F TPMEFNFWLDERQARFYWPIYAWGVLGVAAAVWLAIVADTLFSWLVHNVVAQFQLLKLLLADKERQQAADSDSHLAECI *F RRHRLALELARELSAIFAEIVFVQYMLSYLQLCMLAFRFTRSGWSSQVPFRAAFLVTVFIQLSSYCYGGEYLKQQSSGV *F ALAVYSGCDWSQMPPARRRLWQMMIMRAQRPAKVFGYMFDVDLPLLLWVTRTTGSFLALLRTFER *F Or45b \- GA11917 \- fine as is *F Or46aA \- GA14697-PA \- This is not in BLASTP \- needs to be alternatively *F spliced to the same C-terminal exon as Or46aB, just as in Dmel. Proteins are *F MSKRAEIFYTGQTFIFNIYSLMPQEQRWKRILHEINYWHVMGFWVLLFDLLLVLHVVSNLNNMFEIVRAIFVLATSAGH *F TTKLISVKMNNVALQQLFDRLDDEDFRPEGPEERAIFAAACETTRKTRDYYAALSFAALAMILIPQFVLDWSHLPLGTY *F NPFDDNPGSAGYWLLYCYQCLALSTSCLTNIGFDSLCCSLFIFVNCQLDILALRLQKIGQGKDNDNGNTKMSIDVQLKQ *F CIRFHMAIVDLAETIERRLCTPISMQIFCSVLVLTANFYAIALLSDEKLALFKFVTYQACMLMQIFMLCYFAGEVTHCS *F AELPHRLYNTNWMDWSRSDRRNALLFMQRLHYELRIRTINPSRAFDLALFSSIVNCSYSYFALLKRVNS *F And *F MNQQHLKVTGHFYKYQVWYFQILGIWKLPDGATGQQRRWHQLRFCSIFAILSGMLLLFAMELAGSIAHLREILKVFYMFA *F TEISCMTKLMHLKLKSRKLAGLVTMIKSSSFSTKSEQEEKLMEAGRVSVVNLRNLYGISCLVTATLILLVPFFAGNSELP *F LTMYELCSIEGRMCYWVLFLTHAVSLMSTCCLNIAFESVAYSVLTYLRVQVQMFALRLEQLGPAETPQDNQRIARELREC *F SAHYNRIVQLKDLVEVFIKVPGSVQLMCSILVLVSNLFDMSTISIANGEAIYMTKTCIYQLVMLWQIFIICYASNEVTIH *F SSRLCHSIYKSQWTSWNKENRQMILLMMQRLDSPLCLRTINPTFTFSLEAFGSIVNCSYSYFALLKRVNS *F GA14698 also corresponds to Or46a gene. *F Or47a \- GA12137 \- Needs a few aa on N-terminus *F MSTTENFLLVQKATIAMLGFDLFSGSGEMWKYRHRCINVYSIATIFPFILAAVIHNMKNVMLLADAMVALLITILGLFKF *F SMIIYLRKDFWRMIDTFRHLMTHEGEQGDEYAQIIVTANKQDQRVCGIFRTCFFLAWALNSVLPFVRMGLSYWLSGHVEP *F ELPFPCLFPWDIHNKRNYALTFLWCAFASTGVVLPAVSLDTIFCSFTSNLCAFFKIAQYKVLRFKSETPEESQAKLNKIF *F ALYQKSLDMCTELNHCYEPIICAQFFISSLQLCMLGYLFSITFSQTEGVYYASFIATIIIQAYIYCYCGENVKTESALFE *F WAIYDSPWHESLGSGLESSSICRSLLISMMRASHGFRITGYFFEANMEAFSSIVRTAMSYITMLRSFS *F Or47b \- GA12120 \- Needs internal additions and subtractions. *F MAEPDYTSYLCLLRDFWGEFRSVQRQQTPGRIPRLLMHTQRAALVALCHYPNKKMSSKPVYRRINWILLFNQTLMFISMV *F CGVHESSSIIDMGDDFVWLIGLGLISTKSYCMHARATEIDEVIRDMAYYDEVVRPIHDDEEILMWQRYCYMGEAYFGIGI *F FSLVNAFGLAILLQPLLGEGRLPYHSLLPFGWHRQDLHPWTYRIAFGWLSVNSLHNLSTILFVDLLGISTILQTALNLKL *F LSIELRKLGDLGSVSDNQFHVEFCRVVRYHQHIIRLVDKSNRAFYVTFIAQMIASFAMISISTFETMVAAADDPKMAAKF *F VLFVMVGFVQLSAWCVAGNLVLYLSGEVGQAAFEISDWHTKSVSIQRDIAFIMLRAQKPLFYVARPFKPLSLGTYMIVLK *F QCYRLLALLRESM *F Or49a1 \- GA12084 \- Not in BLASTP \- My model for this duplicated gene is *F MQEKQREYQDFTFLANIMFKTLGYDFLDSARPSWQTGLLRCYFFVCIASSSYEAFFVALECLQVESVAGSPSKIMRRALH *F FFYMLSAAVKFVTLMIYRKRLRTLILSLKELYPADESLRREYEVNKYYLPRSTRYVFYSYYCFMAVMAIGPLPQSFMMYF *F LKGHFPFLRTFPTQLCFRSDTPVGYAVAYFMDLTYSQFVVNVSVGADLWMMCVSSQICMHFGYLAKKLAAYLPSRERERE *F DCEFLASLVQKHQLILRLHKEVNQIFGILLASNLFTTASLLCCIGFYTVVEGRSEEGMSYMIIFVVVSAQFYMVSSFGQQ *F LIDLSSSISMAAYSQYWYDGSLRYKKDLLLIMARAQRPAEISAKGIIIISLDTFKILMTITYRFFAAIRQTVGK *F Or49a2 \- Not in BLASTP and list \- My model for this duplicated gene is *F MKEKEKQCEYQDFIFFANIMFKTLGYDFLDSARPSWQKVLLRCYFFLCIASNCYEASFVALRIIQWESVAGSPSKIMRQA *F LHFFYMLSAEVKFVTLIIYRKRLRTLILGLQELYPTDDSLRREYEVNRYYLPRATRYVLYFYYFVMALMALGPLLQSFTM *F YFLQGNDAKFLFLRIFPTRLSFRVDTPKGYAVAYIMDFTYSQFIVNVSLGTDLWMMCVSSQICMHFGYLAKKLAAYLPSR *F ERERADCEFLCSFVQKHQQILRLHKEVNQVFGLLLASNLFTTASLLCCMAFYTVVQGLNAEGISYMMLFASVAAQFYMVS *F SYGQRLIDLSFSISMAAYLQNWYDGSIRYKKDLLLIMARAQRPAEISAKGIIVISLDTFKILMSITYRFFAVIRQTVGK *F Or49b \- GA14566 \- Fine as is. *F Or56a \- GA11666 \- Needs and open intron removed and a C-terminal exon added. *F MFRVKELLLPRGIFKNPMLRLHLRCFRLYGYVASKYQRRPWLSQARCILFTASIWMSCVLMLARVFQGYERLNDGATTCA *F TALQYFTVSIATMNAIVRRERVVSMLREVHEDMQKLMKEADDQELDLVLSTQKYTKTITLILWVSSIGAGLMALSDCIYR *F TLFMPQTVFNLPAVRRGEERPLLLFRLFPFGELYDNFVVGFLCPWYALGLGVTTIPLWHTFIMCLMKYVHLKLMILNKRV *F PEMDIMRHNPFLDLDRLTPAQLNRWRIRLFTKFVTDHLKIRKFVKELELLICVPVMIDFIIFSILICFLFFALAVGSPTK *F MDYFFMCIYIFVMASILLIYHWHATLISECHDELSFAYYSTPWYEFERSAQRMILFMMIHSQRPLQIRALMIPVNLGTFL *F DIVRAAYSYSNLLRQIY *F Or56N \- Not in BLASTP or list \- Gene downstream of Or56a and lost in Dmel. *F MFRVKELLLPRAIFKSHILGLHLRGIRMYGYVAEKYQRWPLLSLVRCIIFMVSIWVSTVTMLARVFQGFENPNDGVLCWA *F TTIMYISLSISALNSFVQRKRVKGMVRAIHEDIQKLMKEADDQELVLMLSTQKYIRMATWMLWYPALLTGIIAFTDSLYR *F TVLLTLSVFNITERRDEQQYIFLLKVYPFGDVYNNFVFGLFGAWYALGLGINTIPLWNSFIVCLIKYVHLKLLILKKRVT *F EMEITRFNPLLDLDRLTPAQLNRWRMRLIKEFVKEHLKIRRFVKELEQLICLPVLIDFIFFAISICFELYALIVGTPNEM *F EYFLILCYISLTTLILFLTYWHVTLIGECHDDLCFAYYSTPWYEYDPTMKRTILFMMMHAQSPLRIRALMFPVDLKTFLD *F IVLGAYNYFNILRGLY *F Or59a \- GA22057 \- Fine as is. *F Or59b \- GA17527 \- Fine as is. *F Or59c \- GA14401 \- A couple aa missing from C-terminus. *F MKKPLFERLRPVPLTKSVVSSDACIYFYRAATFLGWVPPKARLHRWAYLLWTCTTMVLGLVYLPLGLTLTYVVHFDKFAA *F SEFLTSVQVDINCIGNCVKACVTFSQMWRMRRINAMIAPLDERCPTLNQRQILHKMVARGNRIIVFFLSMYIGFTTTTLF *F SSVFAGKAPWQVYNPLVDWRQGTRQLWEASLLEYIVINIGICQELLSDSYPIVFLSIFRGHLAILKDRIKNLRCNPELSE *F NENYQKLVDCIKDYRTIVQCCDLIRPIMSATIFAQFMLIGIVVGVASVNILFFTTSFWMTLSNIIFIAAICAESFPLCMT *F CELLIEDCESLASGIFHSNWMDAERRYRSAIIYFLHRVQQPIQFWAGAIFPISVQSNITVAKFAFSIITIVNQMNLADKF *F RKEA *F Or63a \- GA22157 \- Needs a couple more aa on C-terminus. *F MYSPEEAAALEKRNYRSIREMIRLSYTVGFNLMRPRRWDVALRIWTVVLSLSSLLSLYGHWQMFRHYVEDMPRIVETVST *F ALQVLTSVFKMWYFLFAHRRIYELLRQARCHELLQRCELFATIADLPVAQVLRRRVAAIMQRYWGSTRRQLLIYLYSVIA *F LTSNYFINSFARNLYRYLTQPPGSFEIVLPLPALYPGWEDKGLAFPYYHIQMYIETCALYICGMCAVSFDGVFIVVCLHG *F VGLMESLGEMIAGATSPLVPPERRVEYLRGCIYQYQRVASFAEEINDCFRHLTLSQFLLSLFGWGLALFQMSVGLGTSSA *F ITMIRMTMYLTASGYQVAVYCYNGQRFATASEQIAGAFYGCEWYAECREFRQLIRMMLTRTGRCFRLDVSWFLAMSLPTL *F MSMVRTSGQYFLLLQNVSEKSG *F Or65a does not appear to have an ortholog in Dp, instead the pair of *F dmOr65b/c is orthologous to a quintuplication in Dp: *F Or65b1 \- GA16875 \- Add 17aa, or even around 100aa, to the N-terminus, e.g. *F MVEGPRDRHGAFGLKSYWNRFIGAFLDARGLLRDPKMVNRHSIAYYSRDQMKVMGLYINAEDKGQPLRRAWHVFLLVQFS *F ALYASMFYGLLKSLDDIVETGRDLAFILGMFFIVFKMVFFSLYADEVDVLIDIMEDSHHAEVKGPGTETCRAIKRHDFLL *F NVGLDFVWVVAVVVFVVLLIVTPFWADQSLPIHAVFPLELHDPAKHPIAHLVIYVCQSFSMAYLNIWLVATEGLSISLYA *F QVTTALSVLCVELQQLRHFWGDSSEDRLRLELTRLVRTHQSIILTVDRCNQLFHGPLIMQMTVNFLLVSLSVFEALMARH *F EPKVAAEFMVLMILALGHLSLWSKFGDMMSQQSLEVAHAAYEAYDPSVGSKRIHRDIGFMVRRSQRPLIMRASPFPAFNL *F SNYMAILNQCYGILTLLLKTLD *F Or65b2 \- Not in BLASTP \- My model is *F MFELPRERVGLGLGEKWKSFMKVYMTFVTVYRSPDECPEHTVPHHCRAQLKAMGYYPNSEERRIPGRRTWFLFLFSQMTM *F FFCSQCYGIFDSMDDLVEWGRDLAFIIASFFIYFKFIYFLLYADNVDEVVDGLEECYRWERAGPAASGVRSAKRLHYLII *F IGMQIIWVVSMVAFVLLLVTTPLWTQQDLPLHVSYPFHLHDSSKHPVTHILIYISQSWSILYFLTGLISTEGLSITIYSQ *F LTTGLTVLCVELRHLHQLCDGDEDLLRWEINRLVKYHQKIISLVDRSNEVFHGPLIMQMIVNFLLVSLSVFEAMMARHDP *F KVAGQFILLMILALGHLSMWTKFGDMMSQESLEVAEAAYEAYDPNVGSKKIDCHIRLIMLRAQEPLIMRATPFPTFNMIN *F YKAILNQCYGILTLLLNTLD *F Or65b3 \- Not in BLASTP \- My model is *F MSELPRERVGLGLGEKWKSFMKLYMTFVTVYRSPDESPEHTVPHQCRAQLKAMGYYPNSEERRIPGRRTWFLFIFSQITM *F FFCSQCYGIFDSLDDLVEWGRDLAFIIASFFIYFKFIYFLLYADNVDEVIDGLEECYRWERAGPAAAGVRSAKRLHYLIV *F IGMQIIWAFSMVVFVLLLVTTPLWTQQDLPLHVSYPFHLHDSSKHPVTHILIYISHSWSILYFLTGLVSTEGLSITIYSQ *F LTTGLTVLCVELRHLHQLCDGDEDLLRWEINRLVKYHQKIISLVDRSNEVFHGPLIMQMIVNFLLVSLSVFEAMMARHDP *F KVAGQFILLMILALGHLSMWTKFGDMMSQESLEVAEAAYEAYDPNVGSKKIDCHIRLIMLRAQEPLIMRATPFPTFNMIN *F YKAILNQCYGILTLLLNTLD *F Or65b4 \- Not in BLASTP \- My model is *F MVEPSGERVGLRLSKKWKRFMKPYAPFRRVYRTPGKCPEHTVPYLNREQLISTGYYPNSTQNSVSGQRSFHLFLLVKGTI *F FYSSILYAASESLDDIVELGRDLAFIIASFFIYFKLIYFLLYADNVDEVVDGLEECYRWERAGPAAAGVRSAKRLHYLIV *F IGMQIIWVVSMIIFIVLLISTPFWTQQELPLHAAYPFHLHDSLRHPRIHILIYLSQSFDILYYLTWLTVTECMSVSIYSQ *F LTTALSVLCVELRHLHQFCDGDEDLLRWEIHRLVKYHQKIIKLVDRCNEVFHGPLIMQMIVNFLLVSLSVFEAMMARHDP *F KVAGQFILLMILALGHLSMWTKFGDMMSQESLEVAEAAYEAYDSSVGSKKIYWNIRFIIMRAQEPLIMRATPFPTFNMTN *F YKAILNQCYGILTLLLNTLD *F Or65b5 \- Not in BLASTP \- My model is *F MVERVGFSLSDKGKSFLKPYIAIRMVYRRPDECPEHTVPYLNRDQLKAIGYYPNSEESSRPGRRNWHLFVMIKATIFFGS *F VTYAIFESLDNIVEWGRDLAFIIASFFIYFKLIYFLLYADNVDEVIDGLEDCYRWERAGPAAAGVRSAKRLHYLIVIGMQ *F IVWLAFMVVFVLLLVTTPLWTELSLPFHAAFPFHWHDPSKHPFTHILIYLSQTFDSAYFLMWLISIEGMSVAIYSQLTTA *F LSVLCVELRHLHQFCDGDEDLLRWEINRLVKYHQKIINLLDRCNEVFHGPLIMQMIVNFLLVSLSVFEAMMARHDPKVAG *F QFIVLMILALGHLSMWTKFGDVMSQESLEVADAAYEAYDPNIASKAVHKDLRVVIMRSQNPLIMRANPFPAFNMINYMAI *F LNQCYGILTLLLNTME *F Or67a \- Nothing in BLASTP or list. My model is *F MSVKFLKEKIFSRRGKSEKPKNAYIVIEDFMKLPIYFYRTIGLNPYELTGTNNKPGIGFHILFLLHMINANMVLALEIFF *F VYVSFRNNENFIESCMVMSYIGFVIVGDLKIGAVLLQKQKLTNLVRQMESVFPPARQKEQEEYDVRRYLRRCLRYTKGFG *F GLYMTLVITYNLFAICQYSIQKWILHSPHAKQSVPYVPLTPWTWQDNWKFYPTYLSQSMAGYTATCGHISADLMIFAVAI *F QVIMHFDRLAKSLTEFTVRAQSEEDGAEKDLKKLQELIAYHNKILGLTDVMNEVFGLALLLNFLASSTLVCFVGFQISIG *F ISPEMLAKLILILISANSEIYLICNFSQMLIDASGSICYAVYDMNWSEADPRFRKMLIVLALRAQKPVCLTATVFLDISI *F ETMSIFLRMSYKFFCAIRTMYQ *F Or67b \- GA12805 \- Fine as is. *F Or67c \- GA12792 \- Needs 8 aa on N-terminus. *F MTGQEQEPDTARTFKDMMRVPVQFYRTIGEDIYAHRSTSPWRSLLLKVYLYGGFINFNLLVIGELVFFYKSIQDFETVRL *F AIAVAPCIGFSLVSDFKQFAMAYYKGTLVRLLDELEEMHPKTLERQRAYRMPDFERTMKRVISIFTFLCLAYTTTFSFYP *F ALKASVKFNLLGYETFDRNFGFLIWFPFDATSSNLVYWIVYWDIAHGAYLAGIAFLCADLLLIVVITQICMHFDYVSRRL *F EEHPCEPGRDRENIEFLVWIVRYHNKCLTLCEHVNNLYSFSLLLNFLMASMQICFIAFQVTESTVEVIIIYCIFLMTSMV *F QVFLVCYYGDTLIATSLRVGDAAYNQKWFQCSKTYCQMLKMLIMRSQRPASIRPPTFPPISLVTYMKVISMSYQFFALLR *F TTYNAN *F Or67d \- GA12793 \- Needs a couple aa on each end. *F MSEGPFERYCKINRAIRFCVGLCGNDVIAEDYRMWWLTYAVIGAILFFFGCTGYTVYVGVVLDGDLTVILQAFALVGSAV *F QGLAKLLVTARMAAVVRQIQATYEAIYREYARRGGDYGRCLERRIKTTWHMLMSFMWVYVVLVGGLIAYPFFHLILHHKK *F LLVMQFRVPWIDESTDGGYLVLISIHVMLLSMGGFGNFGGDMFLFLFISNVPTLKDIFSAKLREFNEVAVRRQDYQRMRT *F LLWDLLAWHQQYVSILRDTERIYRIVLFVQLSTNCVSILCTISCIFIGAWPAAPIYLVYSFIVMYSFCGLGTIVETSNED *F FSKEIYANCLWYELPVKEQRLVILMLAKSQHEISLTAADVMPLSMSTALQLTKGIYSFSMMLITYLGYES *F Or69aA \- No BLASTP and not in list \- My model is *F MQLEDLMRYPDIAFRYFGMAPRFEWTARRTVAPKQTVTRQIVFMVGSLCLGYQNLGMIIYWVRFNSQQKEISMYVAKIAE *F MGSVLALIFAGFLNIWALTSKRAQIEAVLAELQEMYPEPRQRLYRIRHYNDQAVGLMKFTVNFYVVFIIYYNIAPLVLLL *F CEHLMDSQDISYRAQSYTWYPWQVYGSPLGYSAAYLCQAIGSILGVGFSMSSQQLICLFTTQLQLHFDAMANHLTAIDAK *F EPTANQQLRSLILYHRRILRLGDRVNRLFNFTFVVSLIVSTIAICLTSIATMLLELHKALLYISGLIAFVFYHFLICYRG *F SVLTLASDKVMPAAFYNNWYEGDLVYRKMLLILMMRSTKSYVWKTYNLAPVSIQTYMATLKFSYQMFTCVRSLK *F Then there is a pseudogene version of this in between it and Or69aB \- my *F model is *F MMLEDFMQYPDSTCQWIQFRRFEWSGRGSLRPKKSLIKRIIFFAGHHQYGVPCDVIYAYRTERESKNSIMYEADLFSVGS *F SLGFIMEGLCMIGMLIYYRLQIEELLEQLEDLFGIVRKKIYRLGHYEEZWRVMRKSZIIFIVCCTVYNLQSVLILFYEPT *F EAQAVSYRIQRLPLGSXASMVNLGAMMTTMELELHLDGLVRQLEELDAKHPREKEKLRSLIYYHSRILRVADNVNRLFNF *F SVFVSFSTSSLSMCFMFMFMCFTMTVRQLGSALKYMFGLVLFLVYTFSISYNGIZITEASDKVMPAAFYNNWYEGDLVYR *F KMLLILMMRSTKSYVWKTYNLAPVSIQTYMATLKFSYQMFTCVRSLK *F Then Or69aB \- not in BLASTP \- perhaps GA14713 in list? My model is *F MVFISMQLADFMQYSDLGCQVALIRRYGWSGRQSPGAKQTLMKKVIFVLGALNMSCYFFSFITYGYHIERKTKEPIIYVA *F ELSEVGGMLWFTVLGICNMYTLLIYRPQIEELLEGLEQLFAPARQSPYCTRYFYDDSALKMKRLSINFVCSATYYNLLPL *F VKLLSELLTESQQVSYQVQSKAWYPWQVHGSTLGFWIAYASQAFASVMNLGMMMATECLVFVCTAQLELHFDGLARRLEA *F LDARDPRAKEQLRALISYHTRLFKVADRANGIFNCTFLISYCVSSIAVCSMGFSMIMFDLGLALKYMVGMFLFMIYTFCI *F CHNGTQVTMASDKVMPAAFYNNWYEGDLVYRKMLLILMMRSTKSYVWKTYNLAPVSIQTYMATLKFSYQMFTCVRSLK *F GA16694 also corresponds to Or69a gene. *F Or71a \- GA14707 \- Not in BLASTP \- my model is *F MDLDRIRPVRFLLRILRWTRVWPASGGPATQRGWTNWEGYLLHSTLTLIFVVLLWVEAILSPDVEHTVDVLFFTLTMTS *F MCVKVLNSWRYAHVAQRLLTEWSSADRFRLKTLQEVDMWRIGHLRFNRVVGVYMMCSVGVIPVLVTPCLFAVPNKLPFS *F MWTPLDLQQPLGFWTAFSYQAVAIPFACLCDITLNLVNWYLMLHLSLCLRMLGQRLSALHRSGLQQDEEQLCAEFLELV *F SLHRRIKQQALDIETVISKSTFFQILVSSLVICCTVYSLKMTPVIQDMGKFAGLIQYCLSMVLEILSPSIYGNEVTQSA *F DKLPEALYSSDWPDLSPRLRRLILMFMIYLNRPLSLRAGGFFYMGLPMFTKVMNQAYSMLALLFNMNN *F Or74a \- GA12488 \- Fine as is. *F Or82a \- GA16295 \- not in list, but in BLASTP and fine as is. *F Or83a \- GA10437 \- Needs aa on both ends. *F MKERNPPLKIKGDSERRDLFVFVRYTMCIAAMYPFGYSLQGSSFLGLLVRVLDWFYEIFNYFVSVHILGLYICTIYINYG *F QGDLDFFVNCMIQTIIYLWTIAMKLYFRRFRPKMLDEIMSIINENYHTRSALGFSYVTMSGAHHVSKLWIKTYVYCCYIG *F TIFWLVLPIAYRDKSLPLACWYPFDYTQPIVYETVFFLQAIGQIQVAASFASSSGLHMVFCVLLSGQYDVLFCSLKNVLA *F TSYIHMGGNMAELRQLQSEQSIADAEPNQYAYSHEEQTPLEQLLQHPPQDESSRDFLKAFKRSFRHCIDHHRYIVEVLKK *F MERFYSPIWFVKIGEVTFLMCLVAFVSTKSTTANSFMRMVSLGQYLLLVLYELFIICYFADVVYQNSQRSGEALWRSPWQ *F RHLREIRSDYMFFMLNARKQFQLTAGKITNLNVDRFRGTITTAFSFLTLLQKMDARG *F Or83b \- GA10435 \- Fine as is. *F Or83c \- GA13827 \- Needs a N-terminal extension, removal of an open *F inframe intron, and addition of a C-terminal exon. *F MSSPVAVNPAKRFRQLTKNINIWTNLLGVDVLAPKVKFNYRTWTTTFAIVNYTGFTIFSMVDNGGDWRVSLKASLMMGGL *F FHGLGKFLTCLLKQRTMKRLTFFACNIYEEYEGKSEVHYRTLDMNIDRLLGLMRGIRNGYMATFLLMTILPMAMLLYDGT *F RVTVMQYQIPGLPLENNVCYSITYLIQLVTIGVAGCGFYAGDLFVLLGLSQIFAFADILQLKVDDLNAALDRKSEARALV *F SLGATITGEERRQELLLDLIKWHQLFTNYCHTVNELYHDLIATQVLSMAVAVMLSFCIILTSFHMPSAIYFLVSAYSMSV *F YCVLGTKIEFAYDQVYESICSVSWQELSCDQRKLVGPMLREAQNPQSIKLLGILPLSVRTALQIIKLIYSLSMMMMQNRT *F Or85b \- GA11167 \- Fine as is. *F Or85c \- GA14720 \- Mine ends in a K. *F MKFMKYANFFYKAVGIEPYTTDSRPQANSLKASIVFWANVLNLGAIVTGEILYLGVSLADGKLLDAVAVMSYIGFVIVGT *F SKMFFIWWKKPALSDMVRDLEHIYPHGKEAEEEYKLQSYLRSSSRISVTYALLYSVLIWTFNLFSIMQFLVYEKLLHLRV *F VGLALPYTVYYPWNWEAPWSYYMLLFCENFAGYTSAAGQISTDLLLCAVATQVVMHFDHLSTVLEGHELSGKWEEDSRFL *F VNTVKYHQRILRLSEVLNDIFGIPLLLNFMVSTFVICFVGFQMTVGVPPDIMIKLFLFLFSSLCQVYLICHYGQLIADSS *F LGLSNAAYKQNWNHADVRYRRALVFVIARAQKPAHLKATVFMNITRATMTDLLQISYKFFALLRTMYVK *F Or85d \- GA11171 \- Needs aa on each end. And there is also a possible *F long N-terminal extension with no Dm match. I don't know whether to *F include it or not. *F MEGTGKTPSTVEKAEKSEPITTERFLRYANIFYLSIGMEAYDHQGRRKMIELILRCIFIALILNLNAVLLSELIYVFLAI *F GKGTNFLEATMNLSFIGFVIVGDLKVWHIWRKRDQLTNVVREMEKLHPKEGHHQKAYDVESHLSGYSRYSKFYFGMHLVL *F IWTYNLYWAVYYLVCDFWLGIRHFVRMLPYYCWVPWDWSTNSSYYLMYVSQNMAGQTCLSGQLAADLMMCALVTLLVMHF *F IRLGRGIEEHVAGLLSPQQDLEFLQAAVVYHQRLLQLCHNINEIFGVSLLCNFVSSAFIICFVGFQMTIGGKIDNLVMLV *F LFLFCALVQVFMITTYAQRLLDASEHIGEAVYNHDWFQADLPYRKMLIFMVRRSQQASRLKATIFLNVSLVTVSDLLQLS *F YKFFALLRTMYVK *F Or85e \- GA21973 \- Not in BLASTP \- presumably because the Dm ortholog is *F truncated in the genome sequence \- it is a polymorphic pseudogene in Dm. *F Here's my model. Again there is a possible unaligned N-terminal extension. *F MASLQFHGNIDADTRYDATLDPAREPELFRLLMGLQLAMGMNPSPRLPSWWPTWLRPVGGLMAKAYCSMVILTSLHLGLL *F FTKTTLDVLPTGELQAITDALTMTIIYFFTAYANIYWCVRSQRLLAFMDHINREYRHHSLAGVTFVSSHAAHRWSRSFTT *F IWILSCLVGVITWGVSPLMLGIRTLPLTCWYPFDALSPGTYTAVYATQLFGQISVGVTFGFGGSLFVTLCLLLLAQFDVL *F YCSLKNLDAHSKLLSGETIAGLGLLQRELLQGAFTRELNQYAVLQEHATDLLRISAESQNLAQVKVFHSALVECVRLHRF *F ILYCCAELENLFSPYCLVKSMQITLQLCLLVFVGVSGTREFLRIVNQIQYLALTLFELLMFTYCGELLSRHSVRSGEAFW *F RGGWWKHAHLLRQDVLIFLVNSRRAVYVTAGKFYVMDVNRLRSVITQAFSFLTLLQKLAAKKATTEA *F Or85f \- GA14128 \- The final intron has a GC donor, removing three aa *F from your translation. A 72aa unalign N-terminal extension is also *F possible in Dp. *F MESVQRSYEDFPAMPSAVFRLMGYNVLDAPDETRSRRLLMWIYRWLCTCSHAVCVGFMIFRIFEVKTINSIPLIMRYVTL *F VTYVINSDTKHATAMQRESIRNLNKKLADLYPKTTKDRVYYRVNEHYWSRSFLAMICIYIGSSIMVVIGPILQSIFAYFT *F RHQFTYEHCYPYFIYDPNRHPVWVYIIIYATEWLHSTHMVISNVATDLWLLCFQVQICMHFSCMTRSLEEYQPDRTHDVD *F DNRFLAQMVNKHEYLVILQNDLNGIFGGSLLLSLITTSAVICTVSVYSLIQGLTLEGITYVFFIGTSVMQLFLVCNHGQQ *F LLDLSEDIGHAAYNHNWHKASIAYKKYLLIIIIRAQKPVELSAMGYLPISRDTFKQLMSVTYRGLAMIRQMIE *F Or88a \- GA12932 \- Mine has a Q added on the end. *F Or92a \- Not in BLASTP or list \- My model is *F MLWGKRKEKRELRTFEDLTRFPMAFYKTIGEDLYSDRDKNLVRRYLLRFYLVMGFLNFNAYVVGEIAYFIVHITSTTTLL *F EATAVAPCIGFSFMADFKQFGLTVNRGRLVQLLDDLKALFPTTLETQRAYNVSYYQRHMNQVMTLFTILCMTYTSSFSFY *F PAIKATIKYYFLGSEIFERNYGFHILFPYDAETDLTVYWFSYWGLAHCAYVAGVSYVCVDLLLITTITQLTMHFSYMADT *F LEAYDGDEHTDEENIKYLHNLVVYHSRALDLSEEVNSIFSFTILWNFIAASLVICFAGFQITASNVEDIVLYFIFFSASL *F VQVFVVCYYGDEMISSSSRIGHAAFNQNWLPCSTQYKMILKYIIMRSQKPASIRPPTFPPISFNTFMKVISMSYQFFALL *F RTTYYG *F Or94a \- GA14408 \- Needs a K on the end. *F Or94b \- GA19774 \- Fine as is. *F Or98a1 \- GA18957 \- Not in BLASTP but is in list. My model is below. *F MLNLLKEPTPGNLMSSPEAFKYMKNTMILMGWIPPQGGWTASSIIMSIFIVAVSVVYVPIGVFITFFVELKTLSPSEALS *F VLQVALNAMGFPLKLLFFRLYMWRFYKIEKLLGRMDERCIDSTERSEVHRWVARCNIAYLIYQFIYISYTISTFLTATYS *F GVVPWNIYNPFIDWRESTRNLWIDSVLELMFIIGIVIQTYMIDVFPLLYGLILRAHIKLLRQRVEKLCLDPSQSDDENNE *F ELENCIEDHKLILEYASVIRPVIEPAILVQFMLVGLVLGISLINLYLFADTWAKLAIAAYIVVQIVQTFPFCYTCDLIRE *F DCESLAVAIFHSNWKGSSRRYRSSLIYFLLNAQRTISFNAGSVFPICLNTNIRVAKLAFSVVTFVKHLGLGSR *F Or98a2 \- Not in BLASTP or list \- Moved to another chromosome, but tree *F shows is a Dp-specific duplication. My model is below, but a 17 aa *F N-terminal extension is possible. *F MLINLLKEPLPWNLMSSPEAFKYLEYAMILMGWTPPQEGWKPFRIILTIVISFWWILYVPIGVFITFFVELKTLSPSEAL *F SVLQVGINAGGFPLKMIIMRLNMWRYHKIKELLGRMDKRCINITERLEVHRWVARCNIVYLIYQFMYTAYTLSTFFTAIF *F SGVLPFHLYNPFIDWRESTMNLWIASVLELIPMNGIVSQTYMMDVFPLLYGLILRCHVKLLRQRIENLCSDPRKSDDENN *F EDLVFCIKDHKLILEYVSVMRPVIETIIFVQFLLIGLLLGITMLNLFFFADFWAKLAIAAYINGLIIQTFPFCFTCDLLK *F DDCESLALAIFHSHWKSSSRRYKSSLIYFLHNAQMPLSFTAGSIFPIGLKTNITVAKLAFSVVTFVQQLNIADKLRKE *F Or98a3 \- Not in BLASTP or list \- Moved to another chromosome, but tree *F shows is a Dp-specific duplication. My model is below *F MSSPEAFKYLEYAMILMGWTPPQEGWKPFRIILTIVISFWWILYVPIGVFITFFVELKTLSPSEALSVLQVGINAGGFPL *F KMIIMRLNMWRYHKIKELLGRMDKRCINITERLEVHRWVARCNIVYLIYQFMYTAYTLSTFFTAIFSGVLPFHLYNPFID *F WRESTMNLWIASVLELIPMNGIVSQTYMMDVFPLLYGMIVRCHVKLLRQRIENLCSDPRKSDDENNEDLVFCIKDHKLIL *F EYVSVMRPVIETIIFVQFLLIGLLLGITMLNLFFFADFWAKLAIAAYINGLIIQTFPFCFTCDLLKDDCESLALAIFHSH *F WKSSSRRYKSSLIYFLHNAQMPLSFTAGSIFPIGLKTNITVAKLAFSVVTFVQQLNIADKLRKE *F Or98b \- GA15044 \- Needs two aa on C-terminus. *F MLTEKFLRLQSFYFRLLGLELLDQQEVQSVGDIRRSICCILAVATFLPLSIAFGLNNIHNMDKLTDTLCSVLVDLLALCK *F IGIFLGLYKDFRRLIQRFHDMLERECHWEVAAKIVARQNDRDQFISSLYTICFLVAGGSACLMSPLHMIIRFWRTAEMEP *F DYPFPSVYPWDNRRFHNYLLSYLWNVFAAFGVALATICVDTLFSSLTHNLCGLFEICRHKMLTFKRRRPVETQQNLRHIF *F QLYGECLELGSSLNGFFRQIVFAQFIAASLHLCVLCYQLSSNLMQPGMLFYAAFMAAILGQVAIYCHGGACVQAESQLFA *F QAIYESDWLPLLLGGRLDVGRSLQIGMVRAQRGCRLDGYFFEANRKTFDLIVRTAMSYVALLRSTS *F OrN \- No GA \# \- This gene was lost from Dm. *F MTFFTQCRVVSATTYKRILPDESQAHCEMERLRELTQRIRPSDVDEGRIGSIELNVWLAQLTGLPLSGLKPETKAESIRI *F LVVSGVVLPLLFCYVVLEIYDLVLNWDNVDIMTQNVVMTLTHVGYWFKVLNTFYYYEDIRRIVFTLKHLTRTCVLSPGQR *F ETFHQVEVENKVVCLFYFCLVVFSSTLAMVMLLIVPDNLAGKRFPYRVHMPHFLPPIVQYLYMGLSIIWISCGIPTIDNV *F NMLFMNQICMHLKILNMAFDVLQRQVDPNIWMVSIVKYHSVLIKLRQRLEQIYRLPVLFQFVSSLLVVAMTAFQAIVGDG *F SGSSVLIYFLFGGVMCQIFLYCWFGNEVFEQSKTLSTSAFGCNWHEFDAQFKRTLLIFMINADRPFLFTAGGFMGLTLTS *F FANILGKSYSIVTVLRHMYGRAH *F Date: Fri, 22 Jul 2005 15:01:12 \-0500 *F From: Hugh Robertson *F To: Peili Zhang *F Cc: bill@morgan.harvard.edu, flybase-help@morgan.harvard.edu *F Subject: Re: Dpse Ors and Grs *F Dear Peili and FlyBase, I've finished the Grs too now, and comments for *F each are below. Almost every model needs at least slight changes, many *F are new. Again I've attached the table from the manuscript for *F additional detail \- the second table in the file is the Grs. Hugh *F Gr2a \- GA14976 \- Needs a D added to the C-terminus *F Gr8a \- GA13680 \- Needs a Q added to the C-terminus *F Gr9a \- GA17078 \- Needs a different C-terminus to align well with DmGr9a *F MSADWLDRCLGGYFQLLALRCSYSKRRAGRLLSNLYLMLVILDLVGQMRSYAHGEEPMVDRMLFFPKAIQAVNVFYKLVH *F ALIALFALVGCQRERRLLQQLPPTHATPGIYRQVALEFLMVVYALWISFFDSLNAGQILENLRYVFSSQAVRARYLQMLL *F LVGRLQAQLEQLQRQLIDCSLDDYQQLRSSYAHLARLCRSLSQLYGPSLLLLNVLVLGDCLIVCNVYFMVEHLEAVPATW *F LVLWQAVYIVVPTLVKIWTVCAACDRFVMGSKILRQQLSDRRGRTSEERSQIEEFVLQIMQDTLQFNVCGIYHLNLQTLA *F SMFFFILEVLVIFLQFVSVIR *F Gr10a \- GA17050 \- but this is the second half of this annotation and *F perhaps the GA17050 number should be retained for the first half, which *F encodes the Or10a ortholog. My model is *F MSAAEEHEPKESFWERHEFKFYKYGHIYAVIYGQVVIDYVPQQPMRRGLKTVLIAYSHLLSLILIVVLPGYFVYHFRTLT *F ETHDRRMQLMLYVSFANTAIKYATVIVTYVANTVHFEAINYRCTVQRQRLETAFVGAPKQPKRSFEFFMYFKFCLINLMM *F LVQVAGIFALYEAADGPSVSQVRLHFAIYAFVLWNYTENMADYFYFVNGSVLKYYRQLHLQLVSLRRQLGGLRPGGMLME *F HCCRLSDRLGELRQRFGEIHDLYSESFQMHQFQLLGLVLATLINNLTNFYAIFNMLAKQSLEEISFPIVIGSVYATGFYI *F DTYIVTLLNEHIKQELEGFALTMRTFAEPPSTVEQRLTQEIEYFSLELLKCRPPMLCGLLNFDRRLIYLIAVTAFCYFIT *F LVQFDLYLRKSS *F Gr10b \- GA11723 \- Needs a few aa on the N-terminus. This gene is also *F truncated at the C-terminus by the end of a contig, hence is missing the *F last exon, which sadly is only available from a single bad read. Here's *F my best version. *F MGLFLSLAGWVRTHSKRTLVYRQLLSAMWARWLYTLLLRIWMIQGLVLGITGHYYSPSRRRLVPSRVLHWYSWLMMLATL *F ALYWGYWLYAQDYFVQGTFRRHGFVQSLSYGTVVLQLIALVVQTVLRMFREQEVCAVFNELMAMRSTVSRVHPQGQPSRF *F YYVVFFGRLFNYLQNLNFSLSILLIVDMRSVSVWDYLSNFYFVYMSLVRDTLLMSYILLLLELGEVLRVNGEHPRTSYAG *F LMRQLQRQERLLRLVRRVHRLYAWQVLATMFFQLYFNMATFYVGYSFLAASSAPVSGFRVWNAKFLLTVLTFITKLFDGL *F LLQIVGERLLAQGNKPCAGPRVEDVTYMQAAQRQMEMASLKRAIPAGSPKKRFLRMVCIDMKGPFPGINCNLSYGIKILP *F LGISPG *F Gr21a \- Not in BLASTP, but GA12646 in list and genome browser \- My model is *F below, starting where aligns with Dm. This is an extraordinarily *F conserved protein, but it has a potential N-terminal extension of 55 aa, *F but these do not align at all with Dm (which has its own potential 47aa *F extension), and Anopheles gambiae, which has a highly conserved *F ortholog, has no possible N-terminal extension. I there judge that this *F is the likely true start codon for this protein. *F MSFWAVSRGLTPPSKVAPMLNPNQRQFLEDEMRYREKLKLVARGDAMDEVYVRKQETVDDPLELDRHDSFYQTTKSLLVL *F FQIMGVMPIHRNPPVKNLPRTGYSWGSKQVMWAIFIYSCQTTVVVLVLRERVKKFVTSPDKRFDEAIYNVIFISLLFTNF *F LLPVASWRHGPQVAIFKNMWTNYQYKFFKTTGSPIVFPNLYPLTWSLCIFSWVLSIAINLSQYFLQPDFRLWYTFAYYPI *F IAMLNCFCSLWYINCNAFGTASRALSDALQTTIRGEKPAQKLTEYRHLWVDLSHMMQQLGRAYSNMYGMYCLVIFFTTII *F ATYGSISEIMDHGATYKEVGLFVIVFYCMGLLYIICNEAHYASRKVGLDFQTKLLNINLTSVDAATQKEVEMLLVAINKN *F PPIMNLDGYANINRELITTNISFMATYLVVLLQFKITEQRRTNSQAA *F Gr22a \- 16376 \- Needs for aa on N-terminus *F MPQQPHRGYRQRVAHFTLKSTLYASWALGLFPFTYDSRTRQLTRSRWLLSYGLVLNLGLIGVVLLPGTEDHRDVRIDMFE *F RNPIIQQVENMVEIISFLTAVAMHLGIFWKSREMVTILNELFFLEKRHFSNLILAHCHQFDKYVIQKCILVVLEVGSSLL *F IYFGVPDSNLVVTRAFCIYLVQVGVLLGVTHFHLAVIYIYRFVWTINGQLLELANQQRRGQKVDPARIKRLFWLYSRLLE *F VNSRLAAIYDIPVTLFMVTLMSANIMIAHVLIIIWINQFSLLDILLLFPQALLINFYDLWLSIAFCELVERTGRQTSDIL *F KLYNDGEDMDEELQRSLSDFALFCSHRRLRFRHCGLFYVNYEMGFRMIITNILYLVFLVQFDYMNLKYK *F Gr22b \- This is the ortholog of the Gr22b/c split in Dm (Gr22b is a *F polymorphic pseudogene in Dm). In your list it is given the GA16572, but *F it is not in BLASTP. My model is *F MLRRREGFAPKLCRLVLQVTLYGSWAFGLFPFTLDPQTRQLRRHRWLLVYGVAINLFLLCIVVLLSEYSEETTQSLEVFQ *F RNHLLGLMNVIIGVLALIASSGIILISFWKSGQALCIFNELLGLEHRHFGCLDVEDSSKFNLYVIQKGMSVVGELMGLVV *F VNYGMPEYTMSYLYLALLCLTQFCVNLVVTQIYLAILYIYRSVWLINRQLLGVVSRLRVDPLSDSSRIPLLLSLYGRLLA *F LHKQMETTFNGQITLILTSALAGNIVVIYFLIVYTVSLGQLSISLAIFPYSLIMNVWDYWLSISVCDLTERTGRHTSTIL *F KLFSDLEHNDEDLERSINEFTWLCSHRRFRFGLYGLCSVNSETGFQMIVTSFMYLLCLVQFDFMNL *F Gr22dP \- Like Dm, this is a pseudogene, but the defect is different, so *F it is independently a pseudogene. My model is *F MFRPHRGCRHKLVYFILYSILYSSWALGIFPFTYVSKERKLRRSKWLLVYGIAFNATLVVLMLRPHGGEGESMANDPKLD *F VFQRNFVLKQISLLLGIGGVITICAMYLRTFWRSRDLCRIYNQLLHLEVTYFKDYSVECPTFDRYVIQKGLLVIGGVAST *F LVIHMGMPNESVSLVXAGSFLLAIHFHLGVAFIYRFVWLINRELLDLANRLRVRPEGSSSRVRFLLTLYGRLLDLNTRLT *F ACYDYQTAMMMAIFLGGNIIVSFYMIVFSVSLSKMSVFVMLIMFPLALINNFLDFWRTGRQTSMILKLFNDMEILDKEME *F RSFAEFALFCSHRRLRFHHCGLFYVNYEMGFQMLVTSVLYLLFLVQFDYMNL *F Gr22e \- GA16578 \- Fine as is. *F Gr22f \- GA16574 \- I consider the pseudoobscura version of this gene to *F have been lost, but it is a close call \- if not then the Gr22b gene *F above is the equivalent. *F Gr23aA \- GA13700 \- In list and browser only. This is an alternatively *F spliced gene like DmOr23a; here are my two isoforms. *F MPREPFTTITWSSDCSMNSFECLTRRCLAGVFWLMGLVPLPPSSQLCSLLLSLAIRCCWIVYLVYLLSIGIGFWSVATES *F VGNVVGTMLFMGSTVLGLLLLLESVLKQKTHRQLEDLRFQSQLQLQRLGRFGRGRQAAYLLPLIGTQFACDLVRVLISAE *F VGTMISPVFLVSLPLMWLLRLRYVQLVQHVMDLNHRSLQLRRSLLALAAGNDLWQPYGVQEWAQLQTLRKTYQRIFECYE *F VLSDCYGWGMLGLHLATSFEFVTNAYWMITGLYEEQNLLILTFNGATAVDFGTPIATLSWYGDAGAENGREIGCLISKLV *F KPLGSRRYNHLVSEFSLQTLHQRFVVTAKDFFSLNLHLLSSMFAAVVTYLVILIQFMFAERSANAYSG *F And *F MFPLSRKQAFARVVLRFLHLTLSALGLTSRRHSRAVQWLQFGCWLSWYTAIWALTVHRATRTEDCDLDCVLRYVLLVCET *F GSHAIIVTNTFLQQDDSDSLECCDPVVGVTVVGLLVPIIAAQYLVCSNLDKFSERVISYYWKTLPSFLGLQFQIIAFIAQ *F VMYVNLRVRLARRQLQALARELACSWPQSKLQAMYLDHQTARLVDLKRRYNELYHLYSRINERYGGSLLIIFIVFFAGFV *F CNAYWLFIDLRTTPSRLYPILQNLGFIVNVALQMSAACWHCQQSYNLGREIGCLISKLVKPLGSRRYNHLVSEFSLQTLH *F QRFVVTAKDFFSLNLHLLSSMFAAVVTYLVILIQFMFAERSANAYSG *F Gr28a \- GA12527 \- not in BLASTP \- here's my model. *F MAFKLWERISQADNVFQALRPLTYISLLGLAPFRLNLNPRKEVQTSTYSFVAGIVHYLFFVLCFVTSGLEGDSIIGYFFQ *F TNITRLGDKTLRLTGILAMSTIFGFTMFKRQRLVSIIQNYIVVDEIFVRLGMKLDYRRILLFSFLISLGMLLFNVIYLCV *F SYGLLVSATISPSFETFTTFALPHINISLMVFKFLCTTDLAKSRFSMLNEILQDILDAHIEQYNALELSPMHSVVNHKRY *F SHRLRNFISTPMKRYSVTSIIRLNPEYAIKQVSNIHNLLCDICHTIEEYFTYPLLGIIAISFLFILFDDFYILEAVLNPK *F RLDVFETDEFFAFFLIQMSWYIVIIILIVESSSRTILEGNQSAAIVHKILNITDDPELRDRLFRLSLQLSHRRVLFTAAG *F LFRLDRTLIFTITGAATCYLIILVQFRATHHMEDAVGANASQLHFLHD *F Gr28b \- GA12528 \- Like Dm28b, this is an alternatively spliced gene like *F other Ors and Grs, where the last two exons are shared by multiple *F alternative first exons. Here are the five proteins in order, A-E. *F MIRSGLKSYRACRRRVGDALSARDYYGAIQMLIAIAYVLGITPFVVTHSAKGESGMQQSWYGFVNAISRWVLLAYCYTYI *F NLHNESLIGYFMRNRISQFSTRLHNICGIWSAVFTFIMPLLLRRHLQRFIEDVMEVDRRLDRLRHPVNFNAVFGVATLVL *F ALVALLDTIITVTCLVCLAQMEVRASWQLIFILVYELMAISMTIVMFSLITRTVQRRLNYLHTVLKNLSHQWDSHSLKGV *F VQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKR *F ESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLMNKTKSPEVKEKLQQFSMQLLHLKINFTAAGL *F FNIDRTLYFTISGALTTYLIILLQFTSNSPHNSNGNYYSCCETFNNITNHTN *F MSTALRRVRKYFISSQVYEALRPLFFLTFLYGLTPFHVVRRKMGESYLKMSCFGVFNIFIYICLCGFCYISSLRQGESIV *F GYFFRTEISTIGDRLQIFNGLIAGAVIYTSAILKRCKLLGTLTILHSLDTNFSNIGVRVKYSRIFRYSILVLVFKLLILG *F VYFVGVFRLLVSLDVTPSFCVCMTFFLQHSVVSIAICLFCVIAFSFERRLSIINQVLKNLSHQWDSHSLKGVVQKQRSLQ *F CLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVE *F FVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLMNKTKSPEVKEKLQQFSMQLLHLKINFTAAGLFNIDRTLY *F FTISGALTTYLIILLQFTSNSPHNSNGNYYSCCETFNNITNHTN *F MEAGKDTDTVEIEVESGLCQPLRRRVRRFLSAKQLYECLRPVFHVTYWHGLTSFYISSDSATGRKDIKKTIFGFVNGIIH *F ITLYAICYTLTIFNNCESVASYFFRSRITYFGDMMQIVSGFIGVTVIYLTSIIPNHRLERCLEKFHTMDMQLQTVGIKIM *F YSKVLRFSYMILFSMFMVNICFTCGTFSVLYSSLVAPSMALHFTFLIQHTVISIAIAVFSCFTYLVEMRLVMVNKVLKNL *F SHQWDSHSLKGVVQKQRSLQCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAY *F YVLETLLGKSKRESKFKTVEFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLMNKTKSPEVKEKLQQFSMQL *F LHLKINFTAAGLFNIDRTLYFTISGALTTYLIILLQFTSNSPHNSNGNYYSCCETFNNITNHTN *F MSGFWRELFHPRDAHGSEQTLLLCTYILGLTPFRLRGEAGARHYQLSKIGYLNALIQLSFFSYCFLTALIQQQSIVGYFF *F KSEISQVGESLQKFIGMTGMSILFLCSTIRVRLLIHLCNLISRIDGHLLDVGVVFNYPAIMRLRHSQLFLMSTVQLAYLI *F SSTWMLLRNDVRPSYPAAVAFYVPLIFLLSTVILFGAFLHRLWQHLEALNKVLKNLSHQWDSHSLKGVVQKQRSLQCLDS *F FSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTVEFVTF *F FSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLMNKTKSPEVKEKLQQFSMQLLHLKINFTAAGLFNIDRTLYFTIS *F GALTTYLIILLQFTSNSPHNSNGNYYSCCETFNNITNHTN *F MWLFNRLVYMARPHDVYTCYRVTLWLALWLGIVPYYLTSTSAGSSRLSASYFGYFNIIFRMIVYVVNFVYSALDPRSLMS *F NFFLSDISNVIDGLQKVNGMFGIIAILLISLAKRRKLLHVLAVFDCLERESFPRVGITHHQGPAARRMNRLVLVMTGTTT *F AYITCSFLMISMRDAGTFSISSVISYFSPHFIVCAVCFLAGNLMIKLRIYLSALHKVLKNLSHQWDSHSLKGVVQKQRSL *F QCLDSFSMYTIVTKDPAEIIQESMEIHHLICEAAATANKYFTYQLLTIISIAFLIIVFDAYYVLETLLGKSKRESKFKTV *F EFVTFFSCQMILYLIAIISIVEGSNRAIKKSEKTGGIVHSLMNKTKSPEVKEKLQQFSMQLLHLKINFTAAGLFNIDRTL *F YFTISGALTTYLIILLQFTSNSPHNSNGNYYSCCETFNNITNHTN *F Gr32a \- GA13351 \- Not in BLASTP \- here's mine. *F MSPNTWVIEMPTQKARLHPYPRRISPYRTPSVNRYAFSHETPPPPPPPPPRTLEHPVFEDIRTIMSVLKASGLMPIYEQL *F SSHEVGPPTKTNEFYSFFVRGVVHALTIFNVYSLFTPSSAQLFYSYRETDNVNQWIELLLCILTYTLTVFVCARNTKNIL *F RIMNEILQLDDEVRRQFGANLSQNFGFSVKYLFGIAACQTYIIVLKIYAVDGVITPTSYVLLAFYAVQNGLTATYIVFAS *F ALLRIVYIRFHFINQLLNGYTYAQQQRKKGGHRRQAAGATLMENFPEDSLFIYRMHNKLLRIYKGINDCCNLILVSFLGY *F SFYTVTTNCYNLFVQITAKGMVSSNILQWCFAWLCMHVSLLALLSRSCGLTTREANATSQILARVYAKSKEYQNIIDKFL *F TKSIKQEVQFTAYGFFAIDNSTLFKIFSAVTTYLVILIQFKQLEDSKVEDNIQDQQQT *F Gr33a \- GA14395 \- The first exon on this is nearer and shorter, and *F nicely shared with Dm, which needs updating as well. *F MIQIMNWFSMAIGLIPLNRQQSESNVILDYAMMLLVPVFYLGCYFLINLTHAFGLCFLDACNSVCRLSNNLFMHLGAFLY *F LTVTLMSLYRRKDFFLQFDERLNAIDAVIQKCRHVAEMDRVKVTAVKHSVAYHFTWLFLFCVFAFALYYDIRALYLTFGN *F YAFIPFMVSSFPYLAGSIIQGEFIYHVSVISQRFEQINTLFEKINQEARHRHAPLTVFDIESEGKKQERKNLTPATAMDS *F RGPSFGNEQKLSGEMKRQMAAPPPPPPQGQQKNEEDEMDSSYDEDEDDFDYDNATIAENTGNTSEANLPDLFKLHDKILS *F LSVITNGEFGPQCVPYMAACFVVSIFGIFLETKVNFIVSGKSRLLDYVTYLYVIWSFTTMVVAYIVLRLCCNANNHSKQS *F AMIVHEIMQKKPAFMLSNDLFYNKMKSFTLQFLHWEGYFQFNGIGLFALDYTFIFSTVSAATSYLIVLLQFDMTAILRNE *F GLMS *F Gr36a \- GA16444 \- Fine as is. The other GA models in here should be *F dropped, that is, GA16445 and 16442. This is the single ortholog of a *F triplication of DmGr36a-c. *F Gr36d \- I dropped this from the chemoreceptor superfamily but the name *F is stuck in FlyBase. *F Gr39a \- GA16340 \- This gene is complicated. In Dm this is alternatively *F sliced to give four proteins, each with its own first exon and sharing *F the last 3 exons. In Dp there are seven isoforms similarly structured, *F but two were lost from Dm, while one Dm first exon is duplicated in Dp. *F It would be great to have these letters PA-PG along the chromosome as below: *F MRDALHDLLKYQRRLGLTTVDTEDQNGNCCKLRPNWGTFFQFWLLQGVVVFTCSVFIIFWDHKFEATHTGVANHFAHVLE *F VLEPLSISWLLVWMRLHEGRQVRLLNRLQEMARVCHQVVTIPRWLLRLWLISSVGIVLSCLLYAFTLTGLELVSLVPYGT *F FILRHTYYNYLITFFTAIIFGMEQILMAHRRRIERSLRSTNKRELARSLCAIDEIHLLCETDINYIFGGSLALQMLYIVL *F STASFGYILSLEWFELLTCGAIVLCIFPTMFYSTMPAWSIRLQVEANKTAKILAKVPRTGTGLDRMIEKFLLKNLRQQPI *F LTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F MGQKLLLTFLHYQRYLGLSDLDYSEAGRGYWLHATWYSYAAQFLVAGTFFSALVAALSEPLYYINTGSMTGNIFDNAVMM *F TASVTQLLANLWFRSQQQTQVALLQRLSKIKEHLKVDTVALSSPRRMYRLWVGTWFFYGYMVGSFAASFWLAKPKLSHAL *F TLLGFGLRVMSANFQYTCYSGMVCLLQRLLRAQAEELQILVDTHPIPLEALAKSLRVHDEILMLGQREFVQVYGGVLLFL *F FLYQVMQCVLIFYVSTLKGSLNLRTTLTMSGWLAPMLLYLILPLMVNDVSNQANKTAKILAKVPRTGTGLDRMIEKFLLK *F NLRQQPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F MKLLKFLHYQRYLGLSNLDYSQHQQRYTLRGTCVSYALQFVVAVIFVSAFVSALAESVNYMQTNSLTGNIYDHAVILTVS *F VTQLLANLWFRAHQQAQVTLLRRLSKVMRLLKVNTLALGQPRWVYRLWVAVCLWYAMMIGSFGSSIWLSGMKLSHILTLL *F AFALRLLCANFQFTLYSSMVCVLQRLLSVQGELLQVLLGDPSGISRGALARCLRLHDEILMLSQGEFVQVYGGVLLFLFL *F YQVMECVLIAYVSTLEGFRSMQELARIVCWISPMLVYLILPLMINDLSNQANKTAKILAKVPRTGTGLDRMIEKFLLKNL *F RQQPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F MEDPPSGELCVYYKICRYLGIFCIDYNLSKQRFWLRRSLICYVVHFAVQTYLIGCIAIMVLYWNHAFKEEMTQTGNHFDR *F LVMLLALGMLLVQNAWLIWLQAPHLRIVRKLEFYRRKHLQHLRLQLPKRLLWIIIISNMLYLYNFVKICIFEWLSDATRL *F FVITALGFPIRYLVTSFTMGTYCCMVHLMRHLLLSNQSQISLIISQIQDPKLGSANVLRLRGCLDMHDRLVLLCNVEISL *F VYGFIAWLSWMFASLDVTGVIYLAMVVPSLRNPCVQIVGYLVWLTPSLMTCGASFMSNRVALQANKTAKILAKVPRTGTG *F LDRMIEKFLLKNLRQQPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F MSLGVELRVYLSSLKCLGLLCITHEPHDSEYRTHASRTDETLALAGLVCSQCIALLALGHAIVQPRVYELPLYTNVGNVY *F YVANYGLTCLTVSLFYAYFYLRRRSFLSLVSVLLYHNRVDLGNCHSRQFLRLYIIFVSQVLLTGLLQMMIMLYCNIDPLH *F SFLLFFFVSFSYMLIALVIAFYTCLVQIVASLVRLYNRDLTAAVHSRAPLSTTLCRLRLLQRNRLLWVCQQHLTSDFGLV *F LTIIIAFLLFSAPAAPFFMVTIVFEIDARLVGMRHLLIPLAVTLLWNLPIVVALLMTLRSDLVGKEANKTAKILAKVPRT *F GTGLDRMIEKFLLKNLRQQPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F MRMHSFGELRTHLRTLRLLGVFRFHIDYDKCVVSSTPSEERVARFYLWGVLWILLNIQTYCTYMPQHFFMVNYNATGNCY *F ALINIRTCNVTTVLIYTMLYVRRCRYARLLETMLRLNRASRDPQSSSLYGIHLTLFVLCMINYGHGYWRAQVRPTSIPIY *F LFQYGFSYMLMGQLVVLFVSFQRILLSSLRCYNRKLLGSRQLSRECREFYEDFRDYNQIIRLCHEDINDCFGLLLLPITG *F YVLVTTPSGPFYLISTLFEGLFRTPWRFAFMFLTCVFWSMPWVTLLVLAMGTTNVQREANKTAKILAKVPRTGTGLDRMI *F EKFLLKNLRQQPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F MSVCRDLRLYLRALKFLGMMCWQFETERCLLQSTPECERYAQVYTVVVLSGTTGALAYAHLQPDRFRMKVYNRTGNFYEA *F VIFRCSCVVLWLLYVCLYLRRHRHMELVQSLLTINRTCLAGSADRQFRNNFVLYGALSGLIFGNHINGYRHAGLDSIALT *F LNVVLYTYAFLVLCLLLVFFVCLKQIMAAGLTHYNEELRQGIASLDVATIFCGRQKIPSLRGRQQILALRGRQQLLALCE *F GELNECFGLLMLPIVALVLLLCPAGPFFLISTVLEGKYGPKQYILMTLTSIFWDVPWMIILFLMLRTNGITVEANKTAKI *F LAKVPRTGTGLDRMIEKFLLKNLRQQPILTAYGFFALDKSTLFKLFTAIFTYMVILVQFKEMENSTKSINKF *F Gr39b \- GA16339 \- Just needs an S on the end. *F Gr43a \- GA14333 \- There is an open intron that needs to be spliced. It *F might also need to be removed from DmGr43a. *F MEISQPSIGIFYISKILALAPYATRKNSKGQYEIRRSWIFTVYSASLTVTLVFLTYRGLLFDANSKIPVRMKSATSKVVT *F ALDVSVVVLAVVSGVYCGLFSLRETLELNARLYRIDNTLNACNYSRRDRWRALGMASVSLVTISVLLGLDVGSWMRKAEE *F MNIEESDTELNVHWYIPFYSLYFILTGLHINFANTAYGLGRRFRRLNQMLSSSFLGEPKEASALKLRKMSTVKTVSTNTT *F MTPTALHSNLTKLSSEMLPNESAAKNKGLLIQTMADNHESLTKCVLLLSNSFGIAVLFILVSCLLHLVATAYFLFLELLS *F TRDNGYLWVQALWILLHSFRLLMVVEPCHLAARESRKTIQIVCAIERKVHEPILAEAVKKFWQQLLVVDSDFSASGLCRV *F NRTILTSFASAIATYLVILIQFQKTNG *F Gr47a \- GA11896 \- This is a pseudogene in Dp \- my best version is *F YCLIHHSLCTTIPEFVSPPZPZHTACIAITCWQGRHGVRKVFKDLLHLERQYFKGQPSGTPLKARYKAEEQGGTDEEVAX *F LLFSKMLRIGSRZIVSTRSALSVCCPATAGSRSPSTPATPKSLDLSLSLRFFVILSYXLSTDITQLREDSLRILLETNRM *F DRMVRSYPRTYVTLELALHPRRVEFLNVFAFDRKLTLTLLAKTLLYAICWLQGDYMTLKR *F Gr47b \- GA15589 \- Here's my model *F MKPRKSSSDGFIYCYGNLYSLLFYWGLVTFRVRTQSDGGAASSPVSVLYAVCVRCFTVCGYLGSVLIKLEDERMAAAMIG *F HLTPVVKVIIMWECLSSSITYVENFLTLDVQRRRHVKLLANMQGFDLDISEEFPSVRWNYQRTRSKYWYGTVIVTICFFS *F FSLSLILNMARCTCGLSSTLLMAGTYTLLTSSLGLVGFVHIAIMDFVRLRLRLIQKLLHQEYEGSTGRDRPQTTVHRRIA *F KLFQFTKRCSHLLAELNAVFGFSLMTCFAYVICQKVLGSEAWDLEYTFMLLHVTLHSYKLIITSTYGYLLKREKRNCLRL *F LGLYSQHFPQQPLARSQVEDFQHWRMHNRQAALIGSSIQLSVSTIFLVYNGMANYVIILVQLLFQQQQIKERQRELGRDV *F DIVGPMGPRTHLD *F Gr57a \- 12290 \- Fine as is. *F Gr58a \- 15816 \- Here's my model. *F MSIGLLLKTFHSYGLGSGLLPAPLRLDLDLDRIHFSKRSHQRRFYLAYTACLNVLLIVLLPCTFPVFMYDESYMRDKLVL *F QWTFNLTNVTRIMAMVACGYLTWTKREPLLQLGEGLARHCHRCRQLENGALHPSGYRELQKRIRGLLRQQVFVLNLSIVS *F GTLLLMRIDTDVRRSNIIMVVVHMLQFIYVSIMMSGLYVICLLLYWQMERVNLALKELCSRLHHEERNALLLSASLARQT *F LHSLGHLVQLHCEGQRLMRSLFGIFDVTIAFLLLKMFVTNVNLLYHAVQFGNDSIDTTSVTKLWGESLIVTHYWTAVLLM *F NLVDNLTRQNGLETGEILRQFSDLELVKREFQLELERFSDHLRCHSTAYKCCGLFVFNKQTSLIYFFSALVNVLVLYQFD *F LKNSVLKIP *F Gr58b \- GA12328 \- Needs two aa on the C-terminus, plus internally seven *F aa need to be added back at the end of the intron. *F MLHPKLGQALRVAYYHALVFGLMGTTLHIRGNSRLIRVEKVSWIYLAYSLVISGGLLLDTYFMVPKAILDGYIHHNIVLQ *F WNFFLMLGLRIVTIFCSYGLVWLQRRQLVKLYVDSRHLWRNYRRLLKRMVDQQDLEKLQLSLTSLFWRQTIVVYGALLCS *F SVVQYQLLSVINRQSLTALCARLSQLLHVLAVKMTFYALLLMLDHQFQAVLLALRTLQRRKGGKQKAKDLRRIAALHLDT *F YHLARHFFGLYDVANAMLFINMCVTTTSILYHAVQYRNQSIPSDGWGNLFGSGLVLFNLCGTLMLMEKLDRVVSSCNVGP *F ALRQFCDLRKISKELQMELEIFSTQVHRNRLAYKICGLVEVNNSACLSYIGSILSHVIILMQFDIRRQQME *F Gr58c \- GA12324 \- Needs five aa on the N-terminus, plus a C-terminal exon. *F MVHVSLLRFYFELSRLIGLCNLHYDPPHHRLVLNHVPTVVYCLVLDVAYVLIMPFAFSLLVGNIYGCKQLGMFDTVYNVM *F GQAKLFSMLVLIGGVWLRRCRMEGLGNEYLKLLFHFRSAALNHVRRLCLWKVALTSSRFVMLIQILLTPNSLMHCKYTLD *F RTGVAPFYLAAMGYALIMELMVTYVDVTVYMIHVSGNWLISSMTERLQEMIDDVEVLPKRLGRPRDMGLRQILSAWLLLW *F HRCLHLDDLLKQLRDIFQWQILFNLGTTYIFSIATVFRLWIYIDYSKDFSLWTCLIMLFVFLAHHCEVMMQFSIFHTNTS *F KWRKLQEQLQHLWFLNQSQNGAGLTAEVVLSRKLEFAILYLNRKLQARPQRVRHLHILGLFDLSRASGHAMTSSVFSNAL *F VLCQIAYKIYG *F Gr59a1 \- GA15713 \- Please use this for the first of these three *F duplicated genes. *F MGILLMLDIFQWFAVLIGLTSYRPVDDRFIQTRIAKAYTLILNVITVTMLPVALMSAVNYFYVAVWLPRFMWITPFVLYA *F VNYVVIVQTLISRCHRDSILMELHHLVVKLNREMGRAEKKMNSKLRRLFYVKTLTTSYLSLCYILGTFLFSDELTFSMML *F SAFLINNGYNILIATTHFYFVSFWQVARGYDFVNQQLEELISTPSPLTSRYTEEMRSLWSFHSSLGQTAHKINRIYGRQM *F LASRFDYIIFTVINGYIGLMYSSREPTTLFAKCYGGLLYWIRTVDFFMTDYICDLVAQYQSMPKHTASEGVMSNELSSYV *F IYQNSMNLNLKVCGLFPANRKQWLNMMGAILCHSVMLLQYHLMMSAKQRNQ *F Gr59a2 \- *F MGILLMLDIFQWFAVLIGLTSYRLVDDRFIQTRIAKAYTLILNVITVIMLPVALVSMVDYFYVAVWLPRFMWITPFVLYA *F VNYVVIVQTLISRCHRDSILMELHHLVVKLNREMGRAEKKMNSKLRRLFYIKTLTTSYLSLCYVLGTFLSTNELTFSMML *F SAMLINNSYNILIATTHFYFVSFWQVARGYDFVNQQLEELISTRSPLTSGYAEELRNLWSLHGSLSQTAHKINRIYGRQM *F LASRFDYITFTVINGYLGLMYSSSEPTNLFEKCYEGLLYLIRTVDFFMTDYICDLVAQYQSMPKHTASEGVMSNELCSYV *F IYQNSMNLNLKVCGLFSANRKQWLNMMGAILCHSVMLLQYHLMMSAKQHNQ *F Gr59a3 \- *F MRRFLLLDIFQWFAVIIGLTSYRVVDDRFVQTRLSRMYTLIVNVITVTMLPAATLDLFNSFSMGFWLPQFMWITPYVLHA *F VNYAVIVHTLIFRGHRNIIQMELHHLSVKLNREMGRAGKQMNSTLRRLFYLKTLTLSCMCLWHFLQSFIVIGVSSLSKIL *F GLIIINSGFMILMSIVHCYFVSLWKVAIGYDFVNQQLEELIATRSPLTSRYAEELRSLWSLHASLSLTAHKINRIYGRQM *F LASRFEYITFTAVDSYMEIIYYFSESAPAISKCFGISFFSIRTIDFFMADYICDLVAQYQSMPKHTASEGVMSNELSSFV *F IYQNSMNLNLKICGLFPANRKQWLNMMACILGNSAVLMQYHLMMSGKEEKYFKS *F Gr59b \- GA15716 \- Just needs an N on the C-terminus. *F Gr59c \- GA15710 \- Needs an open intron removed. *F MADFVWIIQRFVYLYGRLVGVVNFTVDWRTGRAMITRWATIQAAVQNICLIGLLTFQLLHGDTVLFTFKHAKYLHEYVFL *F MVTAVRHWAVLLTLVSRWRHRGDIVLIWNRLFRATQQRPDVIPLYRRRLILKFIFAVLSDNLHMVLDLSALRQKFSPALV *F LKLIVWYLFTTIFNMIVAQYYLAMLQVNVSYTLIKRDLRELLTETQALCGSTNRRGGVFVTKCCALSDRLDRIAETQSKL *F QALVDGMSKIFQIQSFSMTIVYYLSTIGTIYFAFCTIKYSSTGLGASNWGLLLIVLSTTFFYADNFITINIGFIIMDSNP *F ELMKMLEERTLLCEELDERLKSSFESFQLQLARNPLEFYVMGLFKIDRGRIMSMANSLITHSIILIQWELQNN *F Gr59d \- GA15766 \- Needs a C-terminal exon. *F MPDLVKWCVRISYLYGRVTGTLNFEIDLKTGRTRVTKKATIYSALAHVFLITILAHHLWRVKPTSDLLANANALHENVFM *F VVAWMRVSCALAALAGRWYHRRRYMRLISSFRCLYLKNTEVMQHCRRGFVSKCFIATMAESMQFLMALLVVWDRLTFSLL *F IGIWSVMTVTAVMNVIITQYYFALGNARGHYKLLNRDLREVLAEARSLGPKRKRQNGVFITKCCFLADRVDEIAQTQSEL *F QTLIERMSRIYELQVLCLFCTYYLTSVGNAYLLFSIYKYNNITQGWSKLSLLAGATFLVFYYADCLINSYNVFYLLEAHQ *F EMHKLLEQRNLFPWGLDERLESAFDSLELSLARNPLQLHCFGLFKLDRSSAFDVGNSLLINSVLLIQYDMQNY *F Gr59e \- GA17326 \- Needs 5aa on the N-terminus *F MSSSISNRWGNLLLTISRCLAVAPTARQEGRFARWIHCFWCLVLLGYVWTGCIWKCIVFDAEMPTIEKLLYLMEFPGNIT *F ITGFLVYHAVLNCPYARDVETQIHLLIGRQDFGVAQRLYQKHGKRTRHLLVQTIVFHGACIVVDIVNYDFNWWTTWSSNS *F VYNLPALMISLGVLQYALAVHLLWLLKSHLCHCLEQLQKRRRLPQGIVNLDARYDRFFASLVDAGGCSSLVLEELRATYT *F SIDRLHRQLLDKFGLFLLLNFGNSLCSFCEELYMVFNFFERPQWAAGMLLFYRILWLVMHGGRIWVILAVNEQLVEQECQ *F LFLQLNQMEVCGSHLERTINRFLVQLQTSIGQPLLACGVIDLDTLAMGGFVGVLMAIVIFLIQIGLGNKSLMGVALNQSG *F WIYI *F Gr59f \- GA17325 \- Needs a T on the C-terminus. The third intron has a GC *F donor. And for the N-terminus I'm pretty much stumped because I can't *F build a phase 1 intron like Dm and DyGr59f and all the related genes. *F Rather I have a phase 2 intron there. *F MEAFLMSLAVDMGKPKKVPPKHPMTAERLLKGHPSFHQQTRRLYKALHWLLLISVLANTAPIAVLPGRQGIVYRHLHLCW *F MAVSYGWFCLASYWEFVLITLNKVSIDCYLNAMESAIYVVHSASILILTFQWRHRAPAVIDRIVKSDLERGYSINCRQSK *F HFLRVQLSLVLVLACSAFAIDICSQRFVVYKAILSIHSFVMPNIISSLSFIQYYVLLQGIAWRQAAVTESLQSELQHLPC *F PRRWEVQRLRLQHVELTRFTKLVNTAYQYSIVLLIVGCFFNFNLNLFLVYKGIDVPELADWVRWIYMVLWLAMHMGKVYG *F ILYFNHKVQDEQRKCLALLNGVQCVGPDLLDTLNHFVLQLQTNVRQHVVCGVIVLDLKYLSALLVASANFFIFLLQYDVT *F YEALYKLT *F Gr61a \- GA12601 \- Needs 8 aa on the C-terminus. And a 15aa N-terminal *F extension is possible beyond that of DmGr61a. *F MSKAPDSILRRLKVRRQKQRTILAMRWRCAKGGKEFKELDTFYRAIRPYLCVAQLFGIMPLSNVLSRDPQDVKFRLRSVG *F MCFTGLFLLLGGIKTVMQANILFRTGLNAKNMMNLVFLIVGIVNWLNFTGFARSWSKLILPWSSLDILMQFAPYAPSKHS *F LRSKLRLIGCVVGSLAVVDHLLYYASGYYSYHMHIFHCHTNHSRLSFGSYLEKEFSETFELLPYNMFSVCYGFWLNAAFT *F FLWNFMDIFIVLTSIGLAQRFRQFADRVLALQGRQVPDTLWYDIRRDHIRLCELASLVDESMSNIVLMSCANNVYVICNQ *F ALAIFTKLRHPINYVYFWYSLLFLLSRTSLVFMSASKIHDASLLPLRTLYLVPSTHWTEEVQRFVSQLTSEFVGLSGYRL *F FYLTRKSLFGMMATLVTYELMLLQMDAKSHKAGLPDLCA *F Gr63a \- GA13400 \- Needs six aa on the N-terminus. *F MDMKFPHSFSKMANYYRRKKDAVFHNAKPINSGNAQAYLYGVRKYSIGLAERLDADYQPPPSDRKKSSDSTGSNNPEFTP *F SVFYRNIAPVNWFLRIIGVLPIVRRGPARAKFEMSSASFVYSVVFFMLLACYVGYVANNRIHIVRSLSGPFEEAVIAYLF *F LVNILPIMVIPILWWEARKIAKLFNDWDDFEVLYYQISGHSLPLRLRQKALYIAIVLPILSVLSVVITHITMSDLNINQV *F VPYCILDNLTAMLGAWWFLICEAMSTTAHLLAERFQKALKHIGPAAMVADYRVLWLRLSKLTRDTGNAMCYTFVFMSLYL *F FFIITLSIYGLMSQLSEGFGIKDIGLTITALWNIGLLFYICDEAHYASVNVRTNFQKKLLMVELNWMNSDAQTEINMFLR *F ATEMNPSTINCGGFFDVNRSLFKGLLTTMVTYLVVLLQFQISIPTDKGDSDGGTNITVVDMLMDSLGNDMTILSASSSTT *F THSTATSSTTPPPTSAKHGRGHRG *F Gr64a \- GA16796 \- Fine as is. *F Gr64b \- GA16793 \- Fine as is. *F Gr64c \- GA16792 \- Needs MK on the N-terminus. *F Gr64d \- GA17330 \- Mine is *F MEWSAQSVPNKNTLHHAIGYVLVVAQFFGVLPLSGVEPSVPVASVRFRWFSPLNLLPVAALCFVLLDFVLSAKLVIQNGL *F KLYTIGSLSFSVICIFCFGAFLLLAPRWPHIIRRTFECERIFLQSCYNSSIGRRFSQRLRRWAIALLVTALCEHLSYVVS *F AVWSNWKQIRECHLDIDFWQNYFLRERQELFSILPYSTWFALYVEWCTLSMTFVWNFVDIFLILVCRSMQMRFQQLHWRI *F RQHIGRRMSDEFWQEVRYDLLDLNDLLKLYDKELSGLVLVACANNMYFICVQIYHSFQVKGAVLDEVYFWFCLLYVVSRI *F VNMVLAASSIPQEAKQINFTLDEVPTSCWSKELERLSEIFHNEAFALSGKGYFVLNRRLLFTMAATLMVYELVLINQMEG *F EEVQRSICNRGAGSSMSIFFS *F Gr64e \- No GA number \- Mine is *F MARTTGDPVKRQKCIARIKFWRRSRVGSDITLGILKYKVVSNQAQRFQFSKINAFLRRAVRDDYRYSGSFQEAIKPVLII *F AQIFALMPVRGIGSKLAEDLTFAWSSARTYYALAMMISFGVTSGYIVAFMTNISFDFDSVETMVFYGSIFLISMSFLQLA *F TRWPAIAQEWQAVETKLPPLRLGKERRSLAHHIKMITLVATTCSLVEHLLSMTSTMTYSVACPRWPGHPVDNFLYFNFAT *F VFHFVDYSTFLGLLGKVINVLSTFAWNFNDIFVMAVSVALASRFRHLNDYMQREARSATTVGLLDAVQSQFRNLCKLCQV *F VDDGISTITLLCFSNNLYFICGKILKSMQTKPSASHTMYFWFSLTYLLGRTLVLSLYSSSINDESKRPLRIFRMVPREYW *F CDELKRFSEEVHMDTVALTGMKFFRLTRGVVISVAGTIVTYELILLQFNKEETTAFTCENA *F Gr64f \- GA16791 \- Needs an internal segment restored. *F MKFLPAKLERKFRRLKKHSRSSLTRKLDVMHESARKKVIEENCDAYKNQKQSEYECRKRPTKFPGGTRETFLSEGSFHQA *F VGRVLLVAEFFAMMPVKGVTAKHPGDLSFSWRNVRTCFCLVFIASSLANFGLSLFKVLNNPISFNSVKPIIFRGSVLLVL *F IVALRLAQQWPTLMMYWHEVEQGLPQYPSQVGKGQMGHTIRMVMLVGMMLSFAEHLLSMISAIHYARYCNSTSDPIKNYF *F LRTNDEIFYVTSYSTALALWGKFQNVYSTFIWNYMDMFVMIVSIGLAAKFRQLNNDLRNFKGMHMAPSYWSERRIQYRNI *F CVLCDKMDDAISLITMVSFSNNLYFICVQLLRSLNTMPSVAHAVYFYFSLIFLIGRTLAVSLYSASVHDESRLTLRYLRC *F VPKDSWCPEVKRFSEEVISDEVALSGMKFFHLTRKLVLSVAGTIVTYELVLIQFHEDNDLWDCNQSYYS *F Gr65a \- removed from the superfamily as not clearly homologous. *F Gr66a \- GA20169 \- Needs three aa on N-terminus, and a further 20 are *F possible but don't align with DmGr66a. I also have a somewhat different *F internal splice \- but not sure who is right. *F MPPAQTESALPMVQPLLKEFQLLFYISKIAGILPQDLEKFRTKNVLEKSRNGMVYMLAMLIVYVLLYNILIYSFGEEDRT *F LKASQSTLTFVIGLFLTYIGLIMMGTDQLTALRNQGRIGELYERIRQVDERLYKEKCVVDNSHIGGRIRFMLIMTFLFEL *F SILLATYIKLVDYTQWMSVLWIVSAIPTFINTLDKIWFAVSLYALKERFEAINQTLEELVATHEKFKLWLRGDHDTSSRT *F LDSSQPPEYDSNLEYLYRELGGLDMGSLKGSGKNKVAPVSHSMNSFGESIEASDKATHHPISVNMAHESELSNASKVEEK *F LNNLCQVHDEICEIGKAMNELWSYPILSLMAYGFLIFTAQLYFLYCATQFQSIPSLFRSAKNPFITVIALSYTSGKCVYL *F IYLSWKTSLASKRTGISLHKCGVVADDNLLFEIVNHLSLKLLNHSVDFSACGFFTLDMETLYGVSGGITSYLIILIQFNL *F AAQQAKEAIQTFNSLNDTASLVGAATEMDNGSSTLYDLVTTTMLTPTV *F Gr68a \- GA20248 \- Needs EV added to C-terminus. *F Gr77a \- GA16898 \- Not sure why, but this time I extended the N-terminus *F well beyond the Dm alignment. *F MKFNQANSWRNQCSNAPMLQCANAPLPLALIRLCVLDSLWRTAQNASCIHNDMASSSLAGLTFYWLRKVAIAALLVLYGL *F AKVFGLMAASTPRGGHRVRQSLYWRIHGYAMLVFVGCFSPIAFASVYHRMAFLRQNRLLLLIGFNRYVLMLLCAFATVCI *F HTTKQEEIVGCLNQLLRCRRRLMRLMHTPELRQSIDRLSTRGNLLIVGVLIGVFILSPVHTLQILAWDPAVRENFLYVFS *F LLFIYACQLILGLSLGLYVLVLVLLDHLGHHSNQLLARLLADAASLRAPLGCGIIRRRQQLYHSQQTWLALELWRLLHVH *F HQLLALFRSICSLTGLQAVCFVSLVAMECMVHMFFTYFMHYSKFILRKYHRAFPFNYYAMAFVTGLFANLVLVICLTHRM *F ICRFAHTREVLRSGVLALPPGGTVKQLNETLHYYGLYLKNAEHIFAVRACGLFKLNNVLLFCIVEGMLNYLMILIQFDKV *F INK *F Gr85a \- GA16233 \- This gene is truncated by the end of a contig, so I *F built the rest of it from raw traces \- here's my extended version. *F MSSLKRLVQLSFGFFCALNGIVPFYFGFSSGKLHWSRVLAYYRIIHNFIVIGLTIKFITNYWHFHTHVEHSRSKLMELNT *F FTHFTIVLLSLLSCMECAHRQQDRIYGMIEKLLDLDRLSIELGYIAPKSHQRYIGLLVLAITPLLALRLFIHVGLNKIRT *F RLGFDFPCNCFMSECMILGMSSVGFGIMAEICQCWWRLQTGLKRTLLDDSLPDQLNQLLQLQRMFQCLIDITAEFCTVFK *F FVLLCFLVRNVWCGIVIGYMLVRIFCGHGVSELHLYQLYLAFVICIQPLLYSLLLNCLTHTTDSLMETTKEIVRESQGHE *F LLVERSIQWFSLQLAWQHTNVHVYGTYRINRRLVFQSASVILLHVSYMVQSDYRSM *F Gr89a \- GA13339 \- Not in BLASTP \- My version is below, but there is also *F an 18aa N-terminal extension possible beyond the DmGr89a alignment. *F MSRLPHVCGLCLLLWLWQLLALAPFSYSRSRGARCRRLLTLSGVLRWLLLIGLAPLMLWKSAAMYDATNVRHSMIFKNIA *F LAAMTGDVFISLALLGAHLWHRRGLARLLNGLAQLHRKRKLGWGSTLLLWSKLLLSLYELLCNVPFLQGAGSRLPWTQLL *F AYGVQLYVQHVSSVYANGIFGGMLLLLASLDHLEQESPALARLLKRERGWLRLSASFVDLFQLGIFLLVIGYFVNILANM *F YAYMSYFVSQHGVPLTISNYCLIVTIQLYALILAAHLCQVRHGRLRQRCLELGYLPPELTHHQAMAWTPFPLFAPLDSLK *F FSVLGLFTLDHAFWLFLVSYAMNFIVIILQFSLENMQHADDN *F Gr93a \- GA12269 \- This needs N- and C-terminal extensions. *F MDKYSQQKRGGGGVVAEAWSRGLLLTLYRAARVLGLISFRLDREELQLKLPKSGSRNRILETVWRCLVVLTYAGVWPQLS *F AHLITDRPESYADMFALMQSFSVSILALVSFIIQAKGEDKFRTVLNRYLTLYRRICAVTRVDQLFPMKFIVYFLLKLLLT *F IGGCVHEFPPLLKNEHFKDARNMVAVIVGIYMWLGTLFVLDACFMGFLVSGILYEHMAFNILLMLQRMQPIECEEKAVRM *F SKYQRMRLLCDYADELDECASIYSELYGVTIAFRRMLQWQILFYVYYNFISICLILYLCILHYLNANEIALVSLAMATIK *F LFNLILLIMCADYAVRESQKPNRLPLDIVCTDMDQRWDKSVETFLSQQQTQRLEIKVLGFFQLNNEFILLILSAIISYLF *F ILIQFGITGGFEASEEVRKQFNSSSHQIQELLN *F Gr93b \- GA16189 \- Needs 10 aa on N-terminus *F MTGSSSARSTVMPRVSPWLNGPRISAGLLRGCFYYATVFGVATFRIGLQDDASKLRASSRKGYKWLSILIRVLGSCFYGY *F SYGAWADQYTDWYLRLFFGLRLVGCLVCSVIILVLQVCYEKRILHLVNSFFGLFRRLRALTRTVKAGFGGRLELTLLMFK *F LLSLAFVFLAFQWQYSPWVLLTILCDLYTSIGTGMIMHFCFVGYLSIGVLYAELNRYVDHQLRAQLSSLQDQVEEDDIQQ *F QPDVQAHANLDECLAIYEEIHQVTCSFQRLFDLPLFLTLVQNLSAMAMVSYHAIMSREYHFSLWGLVLKLLIDVLLLTLA *F VHGAVSSSRLVRRLSLENYTIGQSKSYHIKFEIFLGRLNHQQLRVCPLGMFEVSNELTLFFLSAMVTYLTFLVQYGIQTK *F QF *F Gr93c \- GA16064 \- Fine as is. *F Gr93N \- GA16188 \- Not in BLASTP. In our opinion the ortholog of the *F DmGr93d was lost in Dp, while the ortholog of this gene was lost in Dm. *F Thus they are paralogs, hence the name Gr93N. This gene is actually more *F similar in sequence and trees with DmGr92a, but that gene was lost from *F Dp. Complicated, I know. *F MFGVLRKMNASKLSAGILLVMYYHAIFMGIFSFKLQRHWISENGQMLMELRALPRPWLMRFYAIYRILAIGILAYFYLPW *F ILRLEIFFERLVHFIRIITATLVCVCILRLQLLHKADSKQLMNTFFRLFRRVRALPSRKTFGYGGRRELVLLSLALICRI *F HELVYILESDRQHFSMARFLSWWCDTFIVFGINMMMQMSFVCYLSIGILYSELNDFVRFQLRSELQALKRPHGLQPRRQH *F LRTVRRKLNECLALYREIYALATTFQKLTDFPFFLSIVHNYTLLGVVIYRLTIVGWFDKHKIQLSILTTKVILDFFLVTM *F AVEGAMTQFRVIRRLSLENCYISDHKDWHTTFDMFVTHLSLYEFRVRVLGLFDVSNELVLIVLSALVTFVIYVVQYRMQS *F TGEAE *F Gr94a \- GA16146 \- Needs aa on both ends. *F MASSIDVTHRRMVKVLTITLILLMTVFGLLANRYDSRRRQSFKLSKVYLAYAILWTTAFAGIYGYQIYQDYIQGQINLRD *F AVSLYSYMNITVAIINYVTQMIMNDTVAKTMSQVPLFQTLKMFHLDNASLLRSIAMALVKSVGFPLILETTFILQQRRLE *F PELSLIWTVYRLLPLIISNLLNNCYFGAMIVVKEILKAINVRLESHRQQVNIMQREDQLKLNTSFYRMQRFCSLADELDR *F LADRYIVIYVNSDKYLSLMSLSIILSLICNLLGMTVGFYSLYYALADTFLGSKPYDGLGALISLVFLFISLTEITMLAHL *F CNNLLVATRRTAIILQEMNLQHADCRYRQAVHSFTLLVTLTKFKIKPMGLYELDMQLISNVFSAVSSFLLILIQADLSHR *F FKMY *F Gr97a \- GA17266 \- Fine as is. *F Gr98a \- GA12669 \- I don't understand how you avoided the 11bp *F frameshifting insertion relative to Dm around position bp90 in this *F gene. My translation is below, with X for the frameshift. Thus I *F consider this to be either a pseudogene or the translation would start *F after this frameshift. *F MRLMSGELDSCSLRRMHRVMKCLGIIPFESXPIQHFYLKVLCNLFMVFVIGTASSWRFSFNYEFTYDFLNDHMSRILDLT *F NFLVLMSAHFTIVMEILWGNRSAEIEQQMEQILHDLRVHLGREVSLKRFRHYSNAIYGSLISRFLLLFAVAVYNNEGLVF *F SAMYSEAVMQLRFTEFSLYCGVALAFHQELCSAGSSLLVELHLTRFDLWPLRRFTLEKLSRLQQIHGRLWQTIRLIERNF *F QRSLSIMLLKFFVDTAALPYWLYLSKIQHTSVSVQYYCATEEFCKLMEIIVPCWLCSRCDLMQRKFRSIFYRLATGRRNG *F QLNAALIRICIQLRQEKYQFSAGGFAYISTEMLGTFLFGMISYIVIGIQFNLNFNASNSSKLAAEAAVTDAPI *F Gr98b \- GA15973 \- This needs aa on both ends, plus there is a possible *F 53aa N-0terminal extension not alignable in Dm. And GA15975 and GA15976 *F which are kept for DmGr93c/d orthologs can be dropped since their *F ortholog in Dp was lost. *F MKGRRRLLAAARPYLQIFSVFALTPPPNFFDRTTNRRLRRFLIVGYGVYSLFLLGILICMSYVNVLALNAEIEQFQLEDF *F TRAMGRVQKVVLASMGIVIHLNMFLNYRRLGHIYEDIADLEMEIDDASQCFGGQPGHNSFRYRLATNCGLWLVALVGLMP *F RFTIEAMGPFVSWPSKILSELVLIMLQLKCLEYCVFVVMVYGLVLRLRHTLRQLQVELADCNQRDMLQALCVALRRNQQL *F LGRVWRLVGELEKYFTLPMMFLFLFNGLTILHVVNWAYINQFNPADCCRYVRLGNCVLLLINPLVACYLSQRCVNAYNSF *F PRILHQIRCLSVANNFPILSMGLREYCLQLQHLRLLFTCGGFFDINLKNFGGLIVTILGYTIILVQFKFQAVAEEKGRFD *F LNNSQSF # *U FBrf0188559 *a Verstreken *b P. *t 2005.8.5 *T personal communication to FlyBase *u *F Subject: RE: Re: Question about Dap160 alleles *F Date: Fri, 5 Aug 2005 16:14:18 \-0500 *F From: 'Verstreken, Patrik' *F To: *F Cc: 'Bellen, Hugo J' *F Dear Beverly, *F The first W is changed to a STOP. Position 2444 in the Dap160RA CDS is changed *F from a G to an A. *F The sequence in question in the wild types is: *F GAG/TGG/TGG/ACA *F In the DAP160EMS allele, the sequence is: *F GAG/TAG/TGG/ACA *F Best regards, *F Patrik *F \-----Original Message----- *F From: Hugo Bellen mailto:hbellen@bcm.tmc.edu *F Sent: Friday, August 05, 2005 4:01 PM *F To: Koh, Tong-Wey; Verstreken, Patrik *F Subject: Fwd: Re: Question about Dap160 alleles *F >Date: Fri, 5 Aug 2005 16:48:51 \-0400 (EDT) *F >From: Beverley Matthews *F >Subject: Re: Question about Dap160 alleles *F >To: hbellen@bcm.tmc.edu *F >Cc: bev@morgan.harvard.edu *F > *F >I should have specified that the W residues in question are aa815 and *F >aa816 of both Dap160 isoforms. Thanks again. *F > *F >Beverley *F > *F > *F > > Dear Dr. Bellen, *F > > *F > > I am a curator for FlyBase and am currently capturing the genomic *F > > sites of the Dap160 mutations to appear in the genome maps in *F > > FlyBase. I have a question about the Dap160EMS allele reported in *F > > Koh et al., 2004, Neuron 43(2): 193--205. *F > > *F > > It is reported as a W to stop mutation that maps to the second SH3 *F > > domain of Dap160. Looking at the second SH3 domain (approx. aa *F > > 783-834) I find two adjacent W residues. I was hoping you could let *F > > me know which Trp residue is the site of the mutation (if either) *F > > and whether it is a TGG \-> TGA or TGG \-> TAG mutation. *F > > *F > > Thank you for your help. *F > > *F > > Sincerely, *F > > *F > > Beverley Matthews *F > > Curator, FlyBase-Harvard *F > > bmatthew@morgan.harvard.edu *F > > *F Hugo J. Bellen *F Investigator, Howard Hughes Medical Institute Professor, Baylor College of *F Medicine One Baylor Plaza, T630 Houston, TX 77030 *F Tel. 713-798-5272 *F Fax. 713-798-3694 *F email. hbellen@bcm.tmc.edu # *U FBrf0188567 *a Dura *b J.M. *t 2005.3 *T personal communication to FlyBase *u *F The following information accompanied a stock donated to the *F Bloomington Stock Center by Jean-Maurice Dura, Institut de Genetique *F Humaine, CNRS Montpellier (3/05). *F P{GMR-GAL4.w-}2 is a second chromosome insertion. # *U FBrf0188570 *a Ng *b J. *t 2005.9.21 *T personal communication to FlyBase *u *F From: Kevin Cook *F To: gm119@gen.cam.ac.uk *F Subject: Ng insertions *F Date: Wed, 21 Sep 2005 10:38:07 \-0500 (16:38 BST) *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Julian Ng, MRC Centre for Developmental *F Neurobiology, King's College (8/05). *F P{UAS-tsr.N}1.7, P{UAS-tsr.S3A}4.1 and P{UAS-tsr.S3E}2.2 are X *F chromosome insertions. *F P{UAS-LIMK1.IR}1.1 is a homozygous viable and fertile, second *F chromosome insertion. *F P{UAS-tsr.N}2.2.1, P{UAS-tsr.S3A}4.2.3 and P{UAS-LIMK1.IR}2.1 are *F third chromosome insertions *F P{UAS-tsr.S3E}3.3.1 is a homozygous viable and fertile, third *F chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188581 *a Garrity *b P. *t 2005.10.16 *T personal communication to FlyBase *u *F From: Kevin Cook *F To: gm119@gen.cam.ac.uk *F Subject: P{UAS-robo.RNAi}3, P{UAS-lea.RNAi}3 and P{UAS-robo3.RNAi}3 *F Date: Sun, 16 Oct 2005 11:44:16 \-0500 (17:44 BST) *F The following information accompanied stocks donated to the Bloomington Stock *F Center by Paul Garrity, Massachusetts Institute of Technology (9/05). *F P{UAS-robo.RNAi}3, P{UAS-lea.RNAi}3 and P{UAS-robo3.RNAi}3 are third *F chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188605 *a Joyce *b E. *t 2005.4.6 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50417. *F Date: Wed, 6 Apr 2005 10:21:14 \-0700 (PDT) *F From: Eric Joyce *F Subject: Re: Helping FlyBase: ADRC-50417 *F To: 'Rachel Drysdale \(Genetics\)' *F Dear Rachel, *F I was wondering if you could help in updating *F flybase's database on CG15329. Our better descriptive *F name is going to be hold'em. We have 3 alleles of *F hold'em. One, g7, which you have already entered into *F the database and two others called g6 and g8. Thank *F you and please let me know if any further information *F is needed. *F Eric *F Date: Thu, 7 Apr 2005 13:48:47 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: ADRC-50417 *F To: rd120@gen.cam.ac.uk, ejoyce20@yahoo.com *F Hi Eric, *F It is certainly fine to have hold'em as the full name for the CG15329 *F gene, though it would be better to have a short symbol that is shorter, *F and does not include the '. *F Here are three suggestions, none of which have been used for any other *F gene *F hdm (my favorite, on the grounds of brevity) *F hldm *F hodm *F I hope you like one of these. Let me know and I'll make the change, *F and make allele records for g6 and g8 too. *F With best regards, *F Rachel. *F Date: Thu, 7 Apr 2005 10:45:41 \-0700 (PDT) *F From: Eric Joyce *F Subject: Re: Helping FlyBase: ADRC-50417 *F To: 'Rachel Drysdale \(Genetics\)' *F Hi Rachel, *F I like your suggestions for hold'em's abbreviation. *F I agree with your favorite 'hdm' so we will go with *F that one. Thank you very much. *F Sincerely, *F Eric # *U FBrf0188606 *a Edwards *b K. *t 2004.12 *T personal communication to FlyBase *u w- version of P{GawB}BxMS1096. *F Date: Fri, 15 Apr 2005 14:25:54 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F From: Kevin Cook *F Subject: Re: w- version of P{GawB}BxMS1096 *F P{GawB-DeltaKE}BxMS1096-KE is a P transposable-induced w- derivative of *F P{GawB}BxMS1096, which probably carries an internal deletion within *F P{GawB}. The screen generating this derivative will be described in Park, *F H., and K. Edwards. ''Marker removal' screen to generate an improved wing *F disc GAL4 driver. Drosophila Information Service 87 (In press). # *U FBrf0188607 *a Pistillo *b D. *t 2005.4 *T personal communication to FlyBase *u *F To: Rachel Drysdale (Genetics) *F Cc: kevin Cook , Ed Ryder *F From: John Roote *F Subject: Fwd: Stock submission *F Date: Fri, 15 Apr 2005 12:37:56 \+0100 *F Dear Rachel, *F As you can see Kevin has 3 new ED stocks in the Bloomers collection, *F which were kindly donated by Daniela Pistillo, who is currently in the *F Dept of Biology at New York University. *F I would like to report that these deletions are now 'real', with the *F proviso that we have not confirmed them by PCR yet. Daniela was able *F to confirm ED4414 genetically by non-complementation with her mutation *F (sorry I don't know it's identity). *F John *F Begin forwarded message: *F > Hi John-- *F > *F > I'm ready to put the following stocks from Daniela Pistillo into the *F > B'ton collection: *F > *F > Df(1)ED6878, w1118 P{w+mW.Scer\FRT.hs3=3'.RS5+3.3'}ED6878/FM7j, *F > B1 *F > w1118; Df(3L)ED4414, P{w+mW.Scer\FRT.hs3=3'.RS5+3.3'}ED4414/TM6C *F > w1118; Df(3R)ED5416, P{w+mW.Scer\FRT.hs3=3'.RS5+3.3'}ED5416/TM6C *F > *F > Could you send a personal communication from DrosDel to FlyBase so *F > that aberration and insertion entries can be made (or converted from *F > 'potential Df' to 'real Df')? I thought it would be more appropriate *F > to come from you guys than from Bloomington. *F > *F > Kevin # *U FBrf0188608 *a Bourouis *b M. *t 2005.3 *T personal communication to FlyBase *u Lk6 and eIF-4E insertions. *F Date: Wed, 13 Apr 2005 15:19:32 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: Lk6 and eIF-4E insertions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Marc Bourouis, Université de Nice (3/05). *F P{UAS-eIF-4E.A}26 is a homozygous viable, second chromosome insertion. *F P{UAS-Lk6.A}1, P{UAS-Lk6.T424D}6 and P{UAS-eIF4E.S251A}8 are homozygous *F viable, third chromosome insertions. *F Kevin *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188609 *a Wellington *b A. *t 2005.3 *T personal communication to FlyBase *u Wellington constructs and insertions. *F Date: Thu, 14 Apr 2005 14:22:22 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Wellington constructs and insertions--2nd version--use this one *F instead *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Andrea Wellington. University of Arizona (3/05). *F P{otu-capu.W} consists of the wild type capu sequence cloned into P{COG}. It *F gives partial rescue of the capu fused dorsal appendage phenotype. *F P{otu-capu.W}5 is a homozygous viable and fertile, third chromosome insertion. *F P{otu-chic.W} consists of the wild type chic sequence cloned into P{COG}. It *F rescues the chic small egg, sterility and premature ooplasmic streaming *F phenotypes. *F P{otu-chic.W}1 is a third chromosome insertion. *F P{otu-chic.Y126Q} consists of the chic sequence with an alternation causing *F a Y126Q amino acid change cloned into P{COG}. It partially rescues the *F chic small egg phenotype. *F P{otu-chic.Y126Q}7 is a homozygous viable and fertile, third chromosome *F insertion. *F P{otu-chic.Y120H} consists of the chic sequence with an alternation causing *F a Y120H amino acid change cloned into P{COG}. It partially rescues the *F chic small egg, sterility and premature ooplasmic streaming phenotypes. *F P{otu-chic.Y120H}3 is a homozygous viable and fertile, third chromosome *F insertion. *F P{otu-chic.Y126R} consists of the chic sequence with an alternation causing *F a Y126R amino acid change cloned into P{COG}. It does not rescue the chic *F small egg, sterility and premature ooplasmic streaming phenotypes. *F P{otu-chic.Y126R}2C is a homozygous viable and fertile, third chromosome *F insertion. *F P{otu-chic.Y126R}4 is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F pP{COG} was described in Robinson & Cooley, 'Examination of the function of *F two kelch proteins generated by stop codon suppression', Development 1997 *F 124(7):1405--1417 (FBrf0093201). *F P{UAS-spir.L.GFP} consists of the sequence for the long isoform of spir *F tagged with GFP cloned into P{UAST}. *F P{UAS-spir.L.GFP}3 is a homozygous viable and fertile, third chromosome *F insertion. *F P{UAS-spir.S.GFP} consists of the sequence for the short isoform of spir *F tagged with GFP cloned into P{UAST}. *F P{UAS-spir.S.GFP}3 is a homozygous viable and fertile, third chromosome *F insertion. *F Both P{UAS-spir.L.GFP} and P{UAS-spir.S.GFP} give partial rescue of the *F spir fused dorsal appendage phenotype when driven by GAL4 expressed from a *F maternally-expressed alpha-Tubulin promoter. *F The long and short spir isoforms were described in Wellington et al., *F 'Spire contains actin binding domains and is related to ascidian posterior *F end mark-5', Development 1999 126(23):5267--5274 (FBrf0124975). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188610 *a Ratnakumar *b K. *c C. *d Desplan *t 2004.3 *T personal communication to FlyBase *u P{ovo-FLP} construct and insertions. *F Date: Thu, 14 Apr 2005 12:11:36 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{ovo-FLP} construct and insertions *F The following information accompanied stocks donated to the *F Bloomington *F Stock Center by Kajan Ratnakumar and Claude Desplan, New York *F University *F (3/04). *F P{ovo-FLP.R} consists of a 1140 bp Sal1 to Hpa1 fragment carrying the *F ovo *F promoter driving FLP expression in a miniwhite-marked vector. *F P{ovo-FLP.R}M1A is a homozygous and hemizygous viable and fertile, X *F chromosome insertion. *F P{ovo-FLP.R}F1B is a second chromosome insertion. *F P{ovo-FLP.R}M1B is a homozygous viable and fertile, second chromosome *F insertion. *F P{ovo-FLP.R}F1A is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188611 *a Bourouis *b M. *t 2005.3 *T personal communication to FlyBase *u P{UAS-S6kII.B}85. *F Date: Wed, 13 Apr 2005 15:31:43 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-S6kII.B}85 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Marc Bourouis, Université de Nice (3/05). *F P{UAS-S6kII.B} consists of a 3040 bp cDNA fragment carrying the S6kII (RSK) *F open reading frame cloned into pP{UAST}. A synonym for this construct is *F UAS-RSK. *F P{UAS-S6kII.B}85 is a homozygous semi-viable, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188612 *a Bourouis *b M. *t 2005.3 *T personal communication to FlyBase *u P{GAL4-ac.13}1. *F Date: Wed, 13 Apr 2005 15:44:04 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{GAL4-ac.13}1 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Marc Bourouis, Université de Nice (3/05). *F P{GAL4-ac.13} consists of a 13 kb fragment of achaete 5' sequence cloned *F into a pP{CaSpeR-GAL4} vector (also known as pC3G4). The construct *F recapitulates the embryonic and adult achaete pattern and rescues ac *F mutants when combined with a UAS-ac construct. *F P{GAL4-ac.13}1 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188613 *a Rusconi *b J.C. *c U. *d Challa *t 2005.4.20 *T personal communication to FlyBase *u CG1216. *F Date: Wed, 20 Apr 2005 09:29:54 \-0400 *F Subject: CG1216 *F Cc: Uma Challa *F To: flybase-updates@morgan.harvard.edu *F From: Jamie Rusconi *F We are currently working on CG1216 and have named it mrityu (meaning *F death in Sanskrit). mrityu (mri) mutant retina display excessive *F apoptosis in the interommatidial cell (hence the name). mri was *F initially identified as a klumpfuss-regulated molecule during apoptosis *F in the retina. Our experiments indicate that mrityu genetically *F interacts with klumpfuss and that mri is expressed in the *F interommatidial cells during the timecourse of apoptosis. This work *F was presented at the National Drosophila Conference in San Diego. *F Thank you, *F Jamie C. Rusconi and Uma Challa *F Biological Sciences *F University at Albany *F Albany, NY 12222 *F jrusconi@albany.edu for correspondence # *U FBrf0188614 *a Bourouis *b M. *t 2005.3 *T personal communication to FlyBase *u P{UAS-sgg.KK83-84MI}2.1 and P{UAS-sgg.KK83-84RK}6. *F Date: Wed, 13 Apr 2005 16:10:40 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-sgg.KK83-84MI}2.1 and P{UAS-sgg.KK83-84RK}6 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Marc Bourouis, Université de Nice (3/05). *F P{UAS-sgg.KK83-84MI} expresses a kinase dead form of sgg protein, *F presumably due to a misfolded kinase. It does not have dominant negative *F activity on wingless signaling. *F P{UAS-sgg.KK83-84RK} expresses a near kinase dead form of sgg protein, *F which has dominant negative activity on wingless signaling. *F P{UAS-sgg.KK83-84MI} and P{UAS-sgg.KK83-84RK} were produced and assayed as *F in Bourouis, 2002, 'Targeted increase in Shaggy activity levels blocks *F wingless signaling', Genesis 34: 99-102 (FBrf0152388). *F P{UAS-sgg.KK83-84MI}2.1 and P{UAS-sgg.KK83-84RK}6 are homozygous viable and *F fertile, third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188615 *a Roote *b J. *t 2005.4.25 *T personal communication to FlyBase *u Dsim/py. *F To: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: D sim/py *F Date: Mon, 25 Apr 2005 17:07:37 \+0100 *F Dear Rachel, *F http://fbserver.gen.cam.ac.uk:7081/.bin/fbidq.html? *F FBgn0012891&content=full-report *F FlyBase Report *F Gene Dsim\py *F ... *F polychaete is on ch 2 and is orthologous to scabrous (JR unpublished). *F ... *F Many thanks, *F John *F From: John Roote *F Subject: Re: D sim/py *F Date: Wed, 27 Apr 2005 14:27:16 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F ... *F The observation is that female *F D mel\sca1 crossed to male D sim\py produce hybrid females with a *F sca-like (py-like) phenotype. *F ... *F John *F ________________________________________________________ *F John Roote *F Department of Genetics *F University of Cambridge *F Downing Street *F Cambridge CB2 3EH *F Tel: \+44 1223 765124 *F Fax: \+44 1223 333992 # *U FBrf0188616 *a Rorth *b P. *c A. *d Macias *t 2005.5.10 *T personal communication to FlyBase *u *F From: Ana Macías *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: 'UAS-Pvf1' *F Date: Tue, 10 May 2005 09:14:48 \-0300 *F Dear Raquel: *F I transcribe below the answer of Dra Rorth, I expect with this we can solve *F the problem. *F Sincerely Ana Macías *F Dear Ana *F I don't think we have published the UAS-PVF1. The vector is UASp2 (UASp *F with new polylinker) and the insert from the pvf1 cDNA LD30334. You can *F cite me and this unpublished data for flybase *F Pernille *F \----- Original Message \----- *F From: 'Rachel Drysdale (Genetics)' *F To: *F Cc: *F Sent: Monday, May 09, 2005 12:43 PM *F Subject: Helping FlyBase: 'UAS-Pvf1' *F > Dear Ana, *F > *F > I am now working on your paper \- getting the data tracked in FlyBase \- *F > *F > FBrf0184038 == Macias et al., 2004, Int. J. Dev. Biol. 48(10): 1087--1094 *F > 'PVF1/PVR signaling and apoptosis promotes the rotation and dorsal closure *F of the Drosophila male terminalia.' *F > *F > and have a question about one of your constructs. You use 'UAS-Pvf1' *F > (e.g. in 'Pvf1 gain of function' section, p1090) and reference *F > FBrf0139607 == Duchek et al., 2001, Cell 107(1): 17--26 *F > in your Materials and Methods. *F > *F > However the FBrf0139607 paper does not include a 'UAS-Pvf1', unless you *F > mean Pvf1EP1624 or Pvf1EPg11235. We do have two 'UAS-Pvf1' *F > constructs known to FlyBase: *F > *F > P{UAS-Pvf1.R} *F > described in *F > FBrf0174570 == Rosin et al., 2004, Development 131(9): 1939--1948 *F > *F > and *F > P{UAS-Pvf1.M} *F > described in *F > FBrf0160786 == McDonald et al., 2003, Development 130(15): 3469--3478 *F > *F > Might one of these be the one you used? *F > *F > Thanks for help, *F > *F > With best regards, *F > *F > Rachel. # *U FBrf0188617 *a Rehmsmeier *b M. *t 2005.5.10 *T personal communication to FlyBase *u Table of Predicted PREs/TREs. *F File deposited: Rehmsmeier.2005.5.10-1.html ; File date: 2005.5.10 ; File *F size: 80451 ; File format: html *F File deposited: Rehmsmeier.2005.5.10-2.txt ; File date: 2005.5.10 ; File size: *F 109956 ; File format: txt # *U FBrf0188618 *a Rusten *b T.E. *t 2005.6 *T personal communication to FlyBase *u *F Date: Mon, 9 May 2005 18:26:49 \-0500 (EST) *F From: Kathy Matthews *F Subject: Rusten insertions *F To: flybase-updates@morgan.harvard.edu *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Tor Erik Rusten, The Norwegian Radium Hospital, Oslo, *F Norway (6 May 2005). *F P{UAS-eGFP-huLC3}1 is a homozygous viable and fertile insertion in *F chromosome 2. This is the insertion symbol used in the stock genotype *F provided by T.E. Rusten. The construct symbol was originally published *F as UAS-GFP-LC3 (FBrf0180118, p. 189). *F P{UAS-eGFP-drAtg5}16 is a homozygous viable and fertile insertion in *F chromosome 3. This is the insertion symbol used in the stock genotype *F provided by T.E. Rusten. The construct symbol was originally published *F as UAS-GFP-Atg5 (FBrf0180118, p. 189). # *U FBrf0188620 *a Hariharan *b I.K. *t 2005.5.18 *T personal communication to FlyBase *u Question about chakra gene. *F Date: Wed, 18 May 2005 11:54:03 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: question about chakra gene *F To: ikh@berkeley.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Iswar, *F there is a gene record in FlyBase for the chakra gene that has become *F marooned. The entire gene record is copied below [FBgn0015920, edited *F out of pc]. It seems likely to me that either this project was dropped *F or that you continued to study chakra but determined that it *F corresponded to another gene and you went on to publish on it under *F another name. If the latter is the case, or you now know which CG *F chakra corresponds to it would be great if you could let me know, so I *F can update the gene record accordingly. *F With thanks for your help, *F and best regards, *F Rachel. *F Date: Wed, 18 May 2005 09:50:35 \-0700 *F Subject: Re: Helping FlyBase: question about chakra gene *F From: 'Iswar K. Hariharan' *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel: *F We realized that Christian Lehner had cloned the same gene concurrently. *F The two labs (ours and Christian's) that had independently identified *F mutations in the same gene wanted to agree on a name for the gene prior to *F publication (our papers were published back-to-back in the Dec 1996 issue of *F Cell) \- so we agreed to use the name dacapo (dap) instead of chakra. *F Therfore chakra became dacapo. *F Best wishes, *F Iswar # *U FBrf0188621 *a Roote *b J. *t 2005.5.23 *T personal communication to FlyBase *u alleles. *F To: Rachel Drysdale (Genetics) *F From: John Roote *F Subject: alleles *F Date: Mon, 23 May 2005 13:04:08 \+0100 *F Rachel, *F I would like to make the following connections please: *F 1) ms(2)35Ci14-107 is a synonym for the Zuker allele *F ms(2)35Ciz5347. However there's an added confusion: *F Hiller et al. 2004 Development 131:5297 state that z5347 (and z5946) *F are alleles of nht, which maps to the same region as ms(2)35Ci (hence *F it was probably our mistake to call z5347 an allele of 35Ci). *F 2) z2067 (also ex-Zuker) is an allele of ms(2)35Ci. (Dan Lindsley and *F John Roote unpublished) *F Thank you *F John # *U FBrf0188622 *a Megraw *b T. *t 2005.5.23 *T personal communication to FlyBase *u C6/laf? *F Date: Mon, 23 May 2005 10:16:43 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: C6/laf? *F To: timothy.megraw@utsouthwestern.edu *F Cc: rd120@gen.cam.ac.uk, rtc26@gen.cam.ac.uk *F Dear Tim, *F Russ Collins, the FlyBase GO curator, spotted the possible identity of *F laf and C6. I enclose the full gene records below. *F Questions which you might be able to answer \- *F Did C6 become known as laf sometime between the two abstracts? *F Do you, by now, know any more about laf? such as which CG it corresponds to? *F Many thanks for your help \- we like to tidy up these loose ends, wherever *F possible *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F \*a C6 *F \*z FBgn0015605 *F *F \# *F \*a laf *F \*z FBgn0020280 *F *F \# *F Date: Mon, 23 May 2005 15:15:37 \-0500 *F From: Tim Megraw *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: C6/laf? *F Hi Rachel, *F I hope all is well with you. *F C6 was the name of a yeast two-hybrid cDNA clone, and the gene was later *F referred to in an abstract as laf. *F The CG designation for laf is CG14660. *F We really do not know much more about this gene, and did not recover any *F mutations in it. *F Thanks, *F Tim # *U FBrf0188623 *a Liebl *b E. *t 2005.5.20 *T personal communication to FlyBase *u Question about AmaR1 allele. *F Cc: 'seeger.9-osu.edu' *F From: Eric Liebl *F Subject: Re: Question about AmaR1 allele *F Date: Fri, 20 May 2005 17:15:02 \-0400 *F To: Beverley Matthews *F Hi Beverley: *F The deletion starts with the first base of the P114 codon and ends with *F the second base of the A123 codon. So these 29 bases are deleted: *F CCGTACGAGTGTCAGGTGCTTGTTTCGGC *F Eric *F On May 20, 2005, at 2:34 PM, Beverley Matthews wrote: *F > *F Dear Dr. Liebl, *F I am a curator for FlyBase and am in the process of curating the *F molecular nature of the Ama mutations that you reported in a 2003 *F Development paper, to appear in the genome maps in FlyBase. *F I have a question about the AmaR1 deletion. From your description, *F in addition to the missense mutation, there is a 29 base deletion *F beginning in codon P114 (CCG), resulting in a frameshift. *F Can you tell me the exact position of the deletion so I can map the *F mutation accurately in FlyBase? *F wt *F 114 *F MetGlyProTyrGluCysGlnValLeuValSerAlaThrGluLys *F ATGGGTCCGTACGAGTGTCAGGTGCTTGTTTCGGCCACGGAGAAG *F Thanks in advance for any help you can offer. Your response will be *F saved as a Personal Communication to FlyBase and used as a reference *F for the mapping of the AmaR1 mutation (in addition to your *F Development *F paper). *F Sincerely, *F Beverley Matthews *F FlyBase-Harvard # *U FBrf0188624 *a Kolodziej *b P. *t 2005.5 *T personal communication to FlyBase *u P{eg-kinesin-lacZ}K42. *F Date: Wed, 25 May 2005 11:26:40 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{eg-kinesin-lacZ}K42 *F The following information was obtained from the stock list of Peter *F Kolodziej, Vanderbilt University (5/05). *F P{eg-kinesin-lacZ}K42 is a third chromosome insertion. *F Isolation of the P{eg-kinesin-lacZ}K42 insertion was described in *F Higashijima et al., Development 1996 122: 527-536 (FBrf0086454). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188625 *a Kolodziej *b P. *t 2005.5 *T personal communication to FlyBase *u *F Date: Thu, 26 May 2005 10:02:24 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: CyO276 *F The following information was obtained from the stock list of Peter *F Kolodziej, Vanderbilt University (5/05). *F P{lacZ-un3}276 is an insertion of a miniwhite-marked construct. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188626 *a Kolodziej *b P. *t 2005.5 *T personal communication to FlyBase *u Genetic elements from Peter Kolodziej's stock list. *F Date: Thu, 26 May 2005 19:33:01 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Genetic elements from Peter Kolodziej's stock list *F The following information was obtained from the stock list of Peter *F Kolodziej, Vanderbilt University (5/05). *F 1. dodeA is an allele of dode (FBgn0017404). *F 2. axeL14 and axeL15 are alleles of axe (FBgn0017417). *F 3. rack1 is an allele of rack (FBgn0015533). *F 4. P{GAL4-elav.L}2 is a second chromosome insertion. P{GAL4-elav.L}3 is a *F homozygous viable and fertile, third chromosome insertion. These *F insertions may be identical to previously-identified and named insertions *F of P{GAL4-elav.L} (FBtp0000743). *F 5. P{UAS-Pak.myr}2 is a homozygous viable insertion of P{UAS-Pak.myr} *F (FBtp0017298). *F 6. P{UAS-CD2::HRP}2 is a second chromosome insertion of P{UAS-CD2::HRP} *F (FBtp0019068). *F 7. P{GAL4-ftz.ng}3 is a third chromosome insertion of P{GAL4-ftz.ng} *F (FBtp0000961). *F 8. P{UASp-baz.S151A}2 is a second chromosome insertion of *F P{UASp-baz.S151A} (FBtp0017975). *F 9. P{UAS-Dmn.D}2 is a homozygous viable and fertile, second chromosome *F insertion of P{UAS-Dmn.D} (FBtp0016939). *F 10. P{UAS-drl}3 is a homozygous viable and fertile, third chromosome *F insertion of P{UAS-drl} (FBtp0007327). *F 11. P{UAS-pod1.GFPmyc}3 is a third chromosome insertion of *F P{UAS-pod1.GFPmyc} (FBtp0019277). *F 12. P{UAS-pod1.R}3 is a third chromosome insertion of P{UAS-pod1.R} *F (FBtp0018324). P{UAS-pod1.R}2 is a homozygous viable and fertile, second *F chromosome insertion. *F 13. P{UAS-fra}3 is a homozygous viable and fertile, third chromosome *F insertion of P{UAS-fra} (FBtp0007296). *F 14. P{UAS-Apc2.GFP}3 is a third chromosome insertion of P{UAS-Apc2.GFP} *F (FBtp0016539). *F 15. P{GAL4-btl.S}2 is a homozygous viable and fertile, second chromosome *F insertion of P{GAL4-btl.S} (FBtp0001208). *F 16. P{7.8sim/lacZ}1 is homozygous and hemizygous viable and fertile, X *F chromosome insertion of P{7.8sim/lacZ} (FBtp0003497). It is likely the X *F insertion described in Nambu et al., Cell 63: 63-75, 1990 (FBrf0051386). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188627 *a Tanda *b S. *t 2005.6.1 *T personal communication to FlyBase *u Helping FlyBase: E(B)2A. *F Date: Mon, 23 May 2005 17:30:17 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: E(B)2A *F To: tanda@ohiou.edu *F Cc: rd120@gen.cam.ac.uk *F Dear Soichi, *F In 1996 you published an abstract about a gene called 'E(B)2A'. *F \*x FBrf0086019 == Tanda et al., 1996, A. Dros. Res. Conf. 37: 25 *F Enhancer of Bar2A function in the hedgehog signaling pathway is essential for *F eye morphogenesis. *F The entire gene record (FBgn0015693) is included at the bottom of this message. *F I am wondering if the gene referred to as 'E(B)2A' corresponds to *F oro:oroshigane (FBgn0020263), also published by you and Epps, and also *F an enhancer of the B phenotype. If so, I will merge the gene records *F in FlyBase, but if not I will leave them separate. *F Thank you very much for your help, *F with my best regards, *F Rachel. *F Date: Wed, 01 Jun 2005 09:29:48 \-0400 *F Subject: Re: Helping FlyBase: E(B)2A *F From: Soichi Tanda *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F As you mentioned, E(B)2A equals to oroshigane. Please merge it to the *F record of oroshigane. *F Thanks, *F Soichi # *U FBrf0188628 *a Hardy *b R. *t 2005.6.1 *T personal communication to FlyBase *u Goodman deletions. *F Date: Wed, 01 Jun 2005 11:10:46 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Goodman deletions *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Bob Hardy, University of California at San Diego (5/05). *F Df(2L)A92 was induced in a nub1 b1 pr1 chromosome. The left *F breakpoint uncovers *F l(2)36Ca and qua. The right breakpoint is proximal to Fas3. *F Df(2L)B11 was induced in a nub1 b1 pr1 chromosome. The left *F breakpoint uncovers rdo, but not CadN. The right breakpoint uncovers Fas3. *F Df(2L)I131 was induced in a nub1 b1 pr1 chromosome. The left *F breakpoint uncovers CadN. The right breakpoint uncovers Fas3, Arr1 and *F kelch, but not ninaD. *F Df(2L)A33 was induced in a nub1 b1 pr1 chromosome. The left *F breakpoint uncovers CadN. The right breakpoint uncovers rdo, but not Fas3, *F Arr1,kel or ninaD. *F All of these deletions were isolated in the lab of Corey Goodman. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188629 *a Deal *b J. *c K. *d Cook *t 2005.6.1 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC110. *F Date: Wed, 01 Jun 2005 11:53:40 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC110 *F Isolation and characterization of Df(2L)BSC110 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC110 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d06631 and PBac{RB}e04284. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F P{XP}d06631/PBac{RB}e04284 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et. *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.RB3}BSC110 from the segment *F of P{XP}d06631 to the left of its FRT site and the segment of *F PBac{RB}d04284 to the right of its FRT site. Exelixis, Inc. determined the *F insertion site of P{XP}d06631 to be at Release 3 genomic coordinate 5022167 *F on chromosome arm 2L and the insertion site of PBac{RB}e04284 to be at *F Release 3 genomic coordinate 5057196 on arm 2L. The Gene Disruption Project *F determined the insertion site of P{XP}d06631 to be at Release 3 genomic *F coordinate 5022166 on arm 2L and the insertion site of PBac{RB}e04284 to be *F at Release 3 genomic coordinate 5057191 on arm 2L. The cytological *F breakpoints of Df(2L)BSC110 predicted from these coordinates are *F 25C1;25C3. It failed to complement Cg25Ck00405, eIF-3p40k09003 and *F Df(2L)BSC51. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188630 *a Deal *b J. *c K. *d Cook *t 2005.6.1 *T personal communication to FlyBase *u *F Date: Wed, 01 Jun 2005 11:53:45 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC112 *F Isolation and characterization of Df(2L)BSC112 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC112 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d02040 and PBac{WH}f07749. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F P{XP}d02040/ PBac{WH}f07749 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC112 from the segment *F of P{XP}d2040 to the left of its FRT site and the segment of PBac{WH}f07749 *F to the right of its FRT site. Exelixis, Inc. determined the insertion site *F of P{XP}d2040 to be at Release 3 genomic coordinate 18585060 on chromosome *F arm 2L and the insertion site of PBac{WH}f07749 to be at Release 3 genomic *F coordinate 18676894 on arm 2L. The Gene Disruption Project determined the *F insertion site of PBac{WH}f07749 to be at Release 3 genomic coordinate *F 18676916 on arm 2L. The cytological breakpoints of Df(2L)BSC112 predicted *F from these coordinates are 36F7;37A1. It failed to complement *F msl-1gamma216, Df(2L)Exel7071 and Df(2L)OD15 for lethality. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188631 *a Deal *b J. *c K. *d Cook *t 2005.6.1 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC111. *F Date: Wed, 01 Jun 2005 11:53:43 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC111 *F Isolation and characterization of Df(2L)BSC111 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC111 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f05484 and P{XP}d05178. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F PBac{WH}f05484/ P{XP}d05178 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et. *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC111 from the segment *F of PBac{WH}f05484 to the left of its FRT site and the segment of *F P{XP}d05178 to the right of its FRT site. Exelixis, Inc. determined the *F insertion site of PBac{WH}f05484 to be at Release 3 genomic coordinate *F 8232502 on chromosome arm 2L and the insertion site of P{XP}d05178 to be at *F Release 3 genomic coordinate 8355073 on arm 2L. The Gene Disruption Project *F determined the insertion site of PBac{WH}f05484 to be at Release 3 genomic *F coordinate 8232497 on arm 2L. The cytological breakpoints of Df(2L)BSC111 *F predicted from these coordinates are 28F5;29B1. It failed to complement *F Btk29Ak00206 and Df(2L)TE29Aa-11. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188632 *a Harris *b A.N. *t 2005.5.26 *T personal communication to FlyBase *u *F Date: Thu, 26 May 2005 16:41:54 \-0400 (EDT) *F From: Beverley Matthews *F Subject: another PC needed *F To: cambridge-curators@morgan.harvard.edu *F Cc: bev@morgan.harvard.edu *F Hello Beverley, *F We cannot say to the nucleotide where the deletion begins or ends *F because there are 3nt (AGG) repeated on each side. For purposes of *F curation you could decide to arbitrarily assign any of those bases as *F occurring before or after the deletion. *F Here is where the deletion occurs relative to the codons you note below, *F shown with the AGG upstream of the deletion (underscored): *F wt *F 741 793 *F GlnGluGlyCysArgMetThr...ArgIleGlyThrValSerPro *F CAGGAGGGCTGTCGCATGACA...CGGATAGGAACTGTGTCGCCT *F \--- \--- *F N11 *F 740 793 *F GlnGlu // ThrValSerPro *F CAGGAGG // AACTGTGTCGCCT *F \--- *F As a result of the deletion the frame is changed after Glu740. I leave *F ThrValSerPro in the original frame above only so you can properly *F identify the sequence. *F Please let me know if this clears things up. Alternatively, you may *F compare the wt sequence for aub exon 9 in X94613 to the N11 sequence in *F AF334412. *F \-Adam *F \-- *F Adam N. Harris, Ph.D. *F Technical Area Manager *F Research & Development *F Invitrogen Corporation *F 1600 Faraday Ave *F Carlsbad, CA 92008 *F TEL (760) 476-6932 *F FAX (760) 602-6665 *F adam.harris@invitrogen.com *F \-----Original Message----- *F From: Beverley Matthews mailto:bmatthew@morgan.harvard.edu *F Sent: Tuesday, May 24, 2005 10:10 AM *F To: Harris, Adam *F Cc: bev@morgan.harvard.edu *F Subject: Question about aubN11 allele *F Dear Dr. Harris, *F I am a curator for FlyBase and am in the process of curating the *F molecular nature of the aub mutations that you reported in a 2001 *F Development paper, to appear in the genome maps in FlyBase. *F I have a question about the aubN11 deletion. From your description, *F there is a 154 base deletion beginning in codon G741, resulting *F in a frameshift. *F Here's the sequence around the start of the deletion. Can you tell me *F the first base of the deletion so I can map the mutation accurately in *F FlyBase? *F wt *F 741 *F GlnGluGlyCysArgMetThr *F CAGGAGGGCTGTCGCATGACA *F Thanks in advance for any help you can offer. Your response will be *F saved as a Personal Communication to FlyBase and used as a reference *F for the mapping of the aubN11 mutation (in addition to your Development *F paper). *F Sincerely, *F Beverley Matthews *F FlyBase-Harvard # *U FBrf0188633 *a Younger *b S. *t 2005.6.3 *T personal communication to FlyBase *u P{GawB}109(2)80, P{GawB}477 and P{GawB}221. *F Date: Fri, 03 Jun 2005 11:06:36 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{GawB}109(2)80, P{GawB}477 and P{GawB}221 *F I received the following note from Susan Younger, University of California *F at San Francisco (6/3/5). *F Gal4109-2-80 is from our Gal4 screen where we hopped a P{w+mW.hs=GawB} *F from the X onto the second or the third starting with either (1) 7020 *F P{GawB}109C1 or (2) 7021 P{GawB}112A as indicated by the first numbers, 109 *F or 112. Bloomington has several others from this screen all starting with *F 109. The second number, as in 109-2, indicated which autosome carries the *F insertion, \-2 or \-3. As for 109-2-80, I mapped this insertion by chromosome *F in situ to 25D and it has been published by our lab in several *F references. It already has a flybase ID: FBti0015554. *F The other two Gal4 lines, 221 and 477, are from a screen done in Ulrike *F Heberlein's lab. They started with the same Gal4 X chromosome lines that we *F did, 109 or 112, a P{w+mW.hs=GawB}. We were able to screen through some *F of them and identified 221 and 447 as lines expressed in the dendrites. We *F have published both of them in a paper by Wes Grueber, Cell 2003 *F 112(6):805-818. They are in flybase: *F P{GAL4}477 FBti0026455 Insertion is on the 2nd chromosome. *F P{GAL4}221 FBti0026457 Insertion is on the 3rd chromosome. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188634 *a Kaminker *b J. *t 2005.6.7 *T personal communication to FlyBase *u Bgb9 and BgbD. *F Date: Mon, 6 Jun 2005 12:44:39 \-0700 (PDT) *F From: Josh Kaminker *F Subject: Re: question about Bgb *F To: Beverley Matthews *F Yes, that looks right to me. *F Josh *F \--- Beverley Matthews *F wrote: *F > Hi Josh, *F > *F > Just to follow up, can you confirm the amino acid *F > positions of the *F > mutations that you reported so we can add the *F > information to the allele *F > reports in FlyBase? *F > *F > Bgb9 E112D *F > *F > BgbD G116R *F > *F > Thanks for your help, *F > *F > Bev *F Date: Wed, 1 Jun 2005 16:47:46 \-0700 (PDT) *F From: Josh Kaminker *F Subject: Re: question about Bgb *F To: Beverley Matthews *F Hi Bev, *F The bgb story: most of the sequencing work was done by *F a postdoc in the lab so I am relying on what I *F remember from his work. The coordinates in the paper *F are relative to the bgb sequence that he cloned from *F wild type (Ore-R) flies. This is a different sequence *F than what was cloned by Gergen's group which was *F missing a large part of the 5' end of the gene (as *F well as containing some indels relative to the wt *F sequence cloned in the Banerjee lab). I think that *F this sequence was supposed to be submitted to NCBI, *F but I don't think that it was. *F Based on my recollection of what happened (over 5-6 *F years ago...?) and trying to piece together these *F mutations on the current sequence, this is what I *F think these data represent: *F Using the sequence derived from the link to fasta off *F the page for bgb at flybase.org: *F http://flybase.org/.bin/fbidq.html?FBtr0072758 *F and with fastA header: *F >FBgn0013753|>ARGS|Bgb.v004|DNA|Reference sequence of *F Bgb == FBgn0013753 *F A801C E->D *F The A in this triplet is the third nucleotide in the *F triplet GAA (E). *F The triplet is from position 4884-4886 and flanking *F sequence including the triplet is CTG GAA CGA *F The triplet changes from GAA to GAC in the mutant *F resulting in an amino acid change of E->D. *F G808A G->R *F The G in this triplet is the first nucleotide in the *F triplet GGA (G). *F The triplet is from position 4896-4898 and flanking *F sequence including the triplet is GAC GGA GTT *F The triplet changes from GGA to AGA and the mutant *F resulting in amino acid change of G->R. *F Josh *F \--- Beverley Matthews *F wrote: *F > Hi Josh, *F > *F > I was hoping you could *F > answer a question *F > about the BgbD and Bgb9 mutations reported in *F > your 2001 Development *F > paper. The curated molecular information about them *F > is the following: *F > *F > FBrf0138356 == Kaminker et al., 2001, Development *F > 128(14): 2639--2648 *F > *F > 'BgbD' *F > Nucleotide substitution: G808A. *F > Amino acid replacement: G?R. *F > *F > 'Bgb9' *F > Nucleotide substitution: A801C. *F > Amino acid replacement: E?D. *F > *F > That's all I could find from the paper as well. *F > I'd like to know what amino acids are changed so I *F > can map the *F > mutations on the genome. Out of curiosity, what are *F > the nucleotide *F > numbers 801 and 808 relatvie to? Hope you are *F > enjoying you relatively *F > new environs. *F > *F > Thanks, *F > *F > *F > Bev # *U FBrf0188635 *a Lewis *b R. *t 2004 *T personal communication to FlyBase *u The Making of a Mutant (Volume I of the Fly Chronicles). *F File deposited: Lewis.2004_1.doc ; File date: 2004 ; File size: 37376 ; File *F format: doc # *U FBrf0188636 *a McKim *b K. *t 2005.6.16 *T personal communication to FlyBase *u Question about c(2)MZ0810 allele. *F Date: Thu, 16 Jun 2005 13:19:11 \-0400 *F From: Kim McKim *F To: Beverley Matthews *F Subject: Re: Question about c(2)MZ0810 allele *F Dear Beverley, *F I had to go back and reexamine the sequence data and found there was *F quite an error in the reporting of this data. The only thing right was *F that a Gln was changed. The Gln in question is position 457. *F The second Q in the sequence *F EDCVQPQLQP is changed by a CAA to TAA change to a STOP codon. *F . *F . *F Kim *F Beverley Matthews wrote: *F >Dear Dr. McKim, *F > *F >I am a curator for FlyBase and am currently capturing the genomic sites *F >of the c(2)M mutations that you reported in your 2003 Current Biology *F >paper, to appear in the genome maps in FlyBase. I have a question about *F >the c(2)MZ0810 allele. The mutation is reported as a Gln to Lys *F >change in codon 436 but there is no glutamine codon at position 436 in *F >the current annotation or in the GenBank cDNA sequence AY060854. The *F >nearest glutamines are at 431 and 444. Can you please clarify the site *F >of the mutation? *F > *F >Amino acid sequence in the region beginning with Q431 *F > *F >QATKCRDKAAHIYQ *F . *F . *F > *F >Thank you for your help, *F > *F >Sincerely, *F >Beverley Matthews *F >Curator, FlyBase-Harvard *F \-- *F Kim McKim, Ph.D. *F Associate Professor *F Waksman Institute *F Rutgers University *F 190 Frelinghuysen Rd *F Piscataway NJ 08854 *F mckim@rci.rutgers.edu *F waksman.rutgers.edu/~mckim # *U FBrf0188637 *a Ashburner *b M. *c J. *d Roote *t 2005.6 *T personal communication to FlyBase *u *F Date: Wed, 15 Jun 2005 13:17:36 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: stan81f1 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Michael Ashburner and John Roote, University of Cambridge *F (6/05). *F The balancer chromosome CyO-b81f1 (FBba0000350) carries a mutation in *F stan (FBgn0024836) called stan81f1. The balancer short name will now be *F CyO, Dp(2;2)b81f1, b81f1 stan81f1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188638 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u Ca-alpha1DUgra. *F Date: Wed, 15 Jun 2005 13:30:38 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Ca-alpha1DUgra *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner (6/05). *F Ca-alpha1DUgra is a recessive mutation found on a Fs(2)Ugr1 chromosome *F obtained from Janos Szabad. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188639 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u l(2)43A mutations. *F Date: Wed, 15 Jun 2005 14:09:00 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: l(2)43A mutations *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner (6/05). *F l(2)42Aa5-6, l(2)42Ab7-4 and l(2)42Ac8-1 are mutations isolated in a *F cn1 bw1 chromosome by Bob Kreber and Barry Ganetzky, University of *F Wisconsin at Madison. *F l(2)42Aa5-6 does not complement l(2)42Aa1C2, but complements l(2)42Ab1CC. *F l(2)42Ab7-4 does not complement l(2)42Ab1CC, but does complement *F l(2)42Aa1C2. *F l(2)42Ac8-1 escapers have curved wings and thin bristles. *F All three loci are deleted by Df(2R)nap9, Df(2R)nap3, Df(2R)nap11, *F Df(2R)nap13, Df(2R)nap12, Df(2R)nap14, Df(2R)nap15, Df(2R)nap8, *F Df(2R)nap10, Df(2R)nap18, Df(2R)nap2, Df(2R)nap5, Df(2R)nap7, Df(2R)nap4, *F Df(2R)nap6, Df(2R)nap16, Df(2R)nap19, In(2R)bwvDe2LCyR, Df(2R)pk-N5 and *F Df(2R)nap14, but not deleted by Df(2R)Drlrv17, Df(2R)Drlrv25, *F Df(2R)Drlrv28, Df(2R)20B, Df(2R)ST1 or Df(2R)M41A4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188640 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u Df(2R)Npk-rv2 and Df(2R)Npk-rv3. *F Date: Wed, 15 Jun 2005 14:29:12 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Df(2R)Npk-rv2 and Df(2R)Npk-rv3 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner (6/05). *F Df(2R)Npk-rv2 deletes nec, sple, pk, pwn, l(2)43Ba and l(2)43Bb, but does *F not delete l(2)42Ea, l(2)42Eb, l(2)42Ec, Eb1, l(2)42Fa, l(2)43Bc, l(2)43Bd, *F cos, hum, so or dpa. *F Df(2R)Npk-rv3 deletes l(2)42Be, l(2)42Bf, l(2)42Bg, l(2)42Bh, l(2)42Bi, *F l(2)42Ca, l(2)42Cb, l(2)42Cc, l(2)42Cd, l(2)42Da, l(2)42Ea, l(2)42Eb, *F l(2)42Ec, Eb1 and l(2)42Fa, but does not delete nec, sple, pk, pwn, *F l(2)43Ba or l(2)43Bb. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu *F Date: Mon, 20 Jun 2005 12:27:21 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Df(2R)Npk-rv2 and Df(2R)Npk-rv3 *F To: jr32@gen.cam.ac.uk *F Hi John, *F Df(2R)Npk-rv2 and Df(2R)Npk-rv3 are new to FB \- so they will be going *F into the abs data with no more info than is included in your pc unless you *F can provide e.g. mutagen, progenitor (I guess from their symbols that *F there is one), cytology, or is there non other than we know they are a *F two break Df. All help cheerfully received, *F Rachel. *F my ref: rd5236 *F From: John Roote *F Subject: Re: Df(2R)Npk-rv2 and Df(2R)Npk-rv3 *F Date: Wed, 22 Jun 2005 14:38:34 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F Npk was EMS induced, weakly dominant nec pk. *F The Npk-rv series were X-ray induced revertants of this dominant *F phenotype. *F I would like to report the others in the series (no longer available): *F Df(2R)Npk-rv1: deletes nec, pk, sple, l(2)43Ba, pwn, l(2)43Bb, *F l(2)43Bc, l(2)43Bd, cos, hum, so, l(2)43Cb, l(2)43Cc, l(2)43Da, *F l(2)43Db, l(2)43De, blow, scra, l(2)43Ed and possibly further distally; *F does not delete l(2)42Fa. *F Df(2R)Npk-rv4: deletes nec, pk, sple, l(2)43Ba, pwn, l(2)43Bb, *F l(2)43Bc; does not delete l(2)42Fa or cos. *F Npk-rv5: mutant for nec and pk *F Npk-rv6: mutant for nec, weak pk *F Npk-rv7: mutant for nec, weak pk. *F . *F . *F Cytology was not carried out on any of them. *F John # *U FBrf0188641 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u Zhr7F. *F Date: Wed, 15 Jun 2005 14:34:21 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Zhr7F *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner (6/05). *F Zhr7F is a mutation whose allelism with Zhr (FBgn0004840) is inferred *F from mapping information. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188642 *a Moberg *b K. *t 2005.6.19 *T personal communication to FlyBase *u Helping FlyBase: ADRC-50306. *F Date: Sun, 19 Jun 2005 11:50:37 \-0400 *F Subject: Re: Helping FlyBase: ADRC-50306 *F From: Ken Moberg *F To: 'Rachel Drysdale (Genetics)' *F Rachel *F As promised, I writing to inform you of which CG corresponds to the erupted *F gene (see email below). *F erupted (ept) = CG9712 = drosophila Tsg101 *F Thank you *F Ken *F Ken Moberg PhD *F Department of Cell Biology *F Emory University School of Medicine *F 615 Michael St, WBRB 435 *F Atlanta, GA 30322 *F USA *F Tel 404-727-3733 *F Fax 404-727-6256 *F On 3/2/05 8:49 AM, 'Rachel Drysdale (Genetics)' wrote: *F > Dear Ken, *F > *F > We are currently curating the abstracts for the upcoming 46th *F > (San Diego) Annual Drosophila Research Conference, for FlyBase. *F > I am writing in connection with your abstract: *F > The novel gene erupted restricts tissue growth non-cell autonomously. *F > *F > You mention a gene symbol that is new to FlyBase, erupted. Do you know *F > which of the Genome Project CG annotations your gene corresponds to? *F > All the CGs have corresponding gene records in FlyBase already and we *F > don't like to make duplicate records for what is actually the same gene *F > unless we can't avoid it. The CG symbols become synonyms when an *F > annotation is named with a more descriptive or functional name. *F > *F > With best wishes, *F > *F > Rachel. *F > *F > *F > *F > \---------------------------------------------------------------------- *F > Rachel Drysdale, Ph.D. *F > *F > FlyBase (Cambridge), *F > Department of Genetics, *F > University of Cambridge, *F > Downing Street, email: rd120@gen.cam.ac.uk *F > Cambridge, CB2 3EH, Ph : 01223-333963 *F > UK. FAX: 01223-333992 *F > *F > FlyBase: http://fly.ebi.ac.uk:7081/ *F > \---------------------------------------------------------------------- *F > # *U FBrf0188643 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u Gpon291. *F Date: Wed, 15 Jun 2005 14:42:55 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Gpon291 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner (6/05). *F Gpon291 is a recessive allele of Gpo (FBgn0001129) isolated by Ross *F MacIntyre, Cornell University. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188644 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u mwh2. *F Date: Wed, 15 Jun 2005 14:51:43 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: mwh2 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner (6/05). *F mwh2 is a loss-of-function allele of mwh (FBgn0002912) isolated in the *F lab of Paul Adler. *F TM3, mwh2 ru1 Sb1 is the balancer short name of a TM3 (FBab0005463) *F variant. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188645 *a Datta *b S. *t 2005.6 *T personal communication to FlyBase *u P{ana+m}1. *F Date: Thu, 16 Jun 2005 11:01:50 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{ana+m}1 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Suma Datta, Texas A&M University (6/05). *F P{ana+m}1 is a homozygous and hemizygous viable and fertile, X chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188646 *a Cho *b K.O. *t 2005.6.23 *T personal communication to FlyBase *u *F > Date: Thu, 23 Jun 2005 15:03:08 \-0500 (EST) *F > From: FB_Auto_Mailer@rail.bio.indiana.edu *F > To: flybase-help@morgan.harvard.edu *F > Subject: FB Help Mail: 014 Dimp, CG9850 *F > *F > >I found recently that there was a sequence mistake in my cDNA *F > sequence, creating a PDZ binding motif at the carboxyl terminal *F > region. I am not sure that the carboxyl terminal region containing *F > PDZ binding motif (~160 aa), which was identified in yeast two hybrid *F > screen using Dlg PDZ domains as bait, is just a pure coincidence or *F > part of alternatively spliced version. Other biochemical and genetic *F > data still support the connection between Dlg and this gene. Taken *F > together, it is better not to use dimp as the gene name at this *F > point. *F > realname: Kyung-Ok Cho *F > reply-to: kcho@bcm.tmc.edu *F > source: FB Help Mail: *F > usersubject: Dimp, CG9850 # *U FBrf0188647 *a Birman *b S. *t 2005.7.10 *T personal communication to FlyBase *u P{Eaat1-GAL4.R}2. *F Date: Sun, 10 Jul 2005 19:18:41 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{Eaat1-GAL4.R}2 *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Serge Birman, Laboratoire de Génétique et Physiologie du *F Développement, CNRS-INSERM-Université de la Méditerranée (6/05). *F P{Eaat1-GAL4.R}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188648 *a Roote *b J. *c M. *d Ashburner *t 2005.6 *T personal communication to FlyBase *u *F Date: Sun, 10 Jul 2005 19:19:54 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Ashburner stocks *F The following information accompanied stocks donated to the Bloomington *F Stock Center by John Roote and Michael Ashburner, University of Cambridge *F (6/05). *F 1. P{UAS-Mmus\Sox2.S}5.2 is a homozygous viable and fertile, third *F chromosome insertion. P{UAS-Mmus\Sox2.S}3.1 is a homozygous viable and *F fertile, second chromosome insertion. *F 2. P{UAS-ve.dC}2 is a homozygous viable and fertile, second chromosome *F insertion. *F 3. P{UAS-pntP2VP16}2 is a homozygous viable and fertile, second *F chromosome insertion. *F 4. P{UAS-D.S}2 is a homozygous viable and fertile, second chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188649 *a Roote *b J. *t 2005.7.11 *T personal communication to FlyBase *u *F Date: Mon, 11 Jul 2005 13:21:10 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: l(2)CA61 *F To: rd120@gen.cam.ac.uk, jr32@mole.bio.cam.ac.uk *F Hi John, *F ... *F How many alleles of l(2)CA61 do you think there are? *F ... *F Rachel. *F From: John Roote *F Subject: Re: l(2)CA61 *F Date: Mon, 11 Jul 2005 13:40:32 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F ... *F The chromosomes I know about which carry a l(2)CA61 allele are: *F Df(2L)b80k *F Df(2L)b88c25 *F esgVS2 *F esgVS8 *F noc35Ba-1 *F noc35Ba-2 *F These 3 pairs are each from the same source (Alexandrov, Pat Simpson *F and Cambridge respectively) and almost certainly the same progenitor. *F > How many alleles of l(2)CA61 do you think there are? *F Minimum 3, maximum 6. Let's say 3? *F John # *U FBrf0188650 *a Gorczyca *b M. *t 2005.7.11 *T personal communication to FlyBase *u GAL4 line confusion. *F Date: Mon, 11 Jul 2005 15:30:07 \-0400 *F To: sian@morgan.harvard.edu *F From: Michael Gorczyca *F Subject: GAL4 line confusion *F C57 and BG57 are the same line. BG57 was the name inadvertently given *F to the stock in our 1996 Neuron paper and corrected to C57 in *F subsequent publications. *F Neuron Volume 17, Issue 4 , October 1996, Pages 627-640 *F Regulation of Synapse Structure and Function by the Drosophila Tumor *F Suppressor Gene dlg. Vivian Budnik, Young-Ho Koh, Bo Guan, Beate Hartmann, *F Colleen Hough, Daniel Woods and Michael Gorczyca *F Mike *F \-- *F Michael G. Gorczyca, Ph. D. *F Department of Neurobiology *F University of Massachusetts Medical School *F Aaron Lazare Medical Research Building \- 760E *F 364 Plantation St. *F Worcester, MA 01605 *F U.S.A. *F phone:(508) 856-4412 *F fax: (508) 856-6636 *F michael.gorczyca@umassmed.edu # *U FBrf0188651 *a Birman *b S. *t 2005.6 *T personal communication to FlyBase *u P{TH-GAL4.F}3. *F Date: Mon, 11 Jul 2005 14:14:39 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Re: P{TH-GAL4.F}3 *F To: rd120@gen.cam.ac.uk, kcook@bio.indiana.edu *F The following information accompanied stocks donated to the Bloomington *F Stock Center by Serge Birman, Laboratoire de Génétique et Physiologie du *F Développement, CNRS-INSERM-Université de la Méditerranée (6/05). *F P{ple-GAL4.F}3 is a homozygous viable and fertile, third chromosome insertion. # *U FBrf0188652 *a Deal *b J. *c K. *d Cook *t 2005.7.11 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC108. *F Date: Mon, 11 Jul 2005 12:36:49 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC108 *F Cc: jedeal@bio.indiana.edu *F Isolation and characterization of Df(2L)BSC108 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC108 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d04594 and PBac{RB}e02576. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F P{XP}d04594/PBac{RB}e02576 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.RB3}BSC108 from the segment *F of P{XP}d04594 to the left of its FRT site and the segment of *F PBac{RB}e02576 to the right of its FRT site. Its presence was verified *F using the PCR methods and primers described in Parks et al with the *F substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus *F or RB3' minus primer in the Hybrid PCR protocol in the Supplementary *F Methods. Exelixis, Inc. determined the insertion site of P{XP}d04594 to be *F at Release 3 genomic coordinate 6866624 on chromosome arm 2L and the *F insertion site of PBac{RB}e02576 to be at Release 3 genomic coordinate *F 6936961 on arm 2L. The Gene Disruption project determined the insertion *F site of P{XP}d04594 to be at Release 3 genomic coordinate 6866623 on arm *F 2L. The cytological breakpoints of Df(2L)BSC108 predicted from these *F coordinates are 27C2;27C6. It failed to complement *F P{SUPor-P}CG10354KG04760, P{PZ}Hrb27C02647, P{SUPor-P}CG18304KG00684 *F and weeES1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188653 *a Van Doren *b M. *t 2005.5.9 *T personal communication to FlyBase *u Dp(2;Y)G, P{hs-hid}Y. *F Date: Fri, 24 Jun 2005 18:12:49 \-0500 (EST) *F From: Kathy Matthews *F Subject: Dp(2;Y)G, P{hs-hid}Y *F To: flybase-updates@morgan.harvard.edu *F Cc: matthewk@fly.bio.indiana.edu *F Personal communication from: Mark Van Doren, Johns Hopkins University *F To: Bloomington Drosophila Stock Center *F Subject: Dp(2;Y)G, P{hs-hid}Y *F Dated: 9 May 2005 *F Background: The FlyBase record for P{hs-hid}Y (FBti0017539) is *F incomplete. The insertion was generated by Mark Van Doren, and he *F provided the following information when donating the insertion to *F the stock center. *F Information communicated: *F A conditional virginator stock was made by jumping P{hs-hid} onto *F the Dp(2;Y)G modified Y. The stock produces few males due to the *F duplication, but is generally healthy despite this (may cause problems *F when combined with other backgrounds). The modified Y also causes some *F increased non-disjunction so incidence of XO males is increased. As *F these are sterile, they do not interfere with the virginator aspects *F of the stock. # *U FBrf0188654 *a Hales *b K. *t 2005.7.19 *T personal communication to FlyBase *u Additional fzo allele. *F Date: Tue, 19 Jul 2005 15:47:28 \-0400 *F Subject: additional fzo allele *F From: 'Hales, Karen' *F To: *F A new allele of fzo, called fzoz3-4436 was found within the Zuker *F collection. This allele failed to complement fzo1. The Zuker collection is *F described in Koundakjian EJ, Cowan DM, Hardy RW, Becker AH. 2004. The Zuker *F collection: a resource for the analysis of autosomal gene function in *F Drosophila melanogaster. Genetics. 2004 May;167(1):203-6. *F Karen Hales # *U FBrf0188655 *a Andrade *b R. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC113. *F Date: Fri, 22 Jul 2005 14:51:24 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC113 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(3L)BSC113 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC113 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d07693 and PBac{RB}e04497. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; *F P{XP}d07693/PBac{RB}e04497 females crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO males. These females were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.RB3}BSC113 from the segment *F of P{XP}d07693 to the left of its FRT site and the segment of *F PBac{RB}e04497 to the right of its FRT site. Its presence was verified *F using the PCR methods and primers described in Parks et al with the *F substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus *F or RB3' minus primer in the Hybrid PCR protocol in the Supplementary *F Methods. Exelixis, Inc. determined the insertion site of P{XP}d07693 to be *F at Release 3 genomic coordinate 9308161 on chromosome arm 3L and the *F insertion site of PBac{RB}e04497 to be at Release 3 genomic coordinate *F 9380896 on arm 3L. The Gene Disruption Project determined the insertion *F site of P{XP}d07693 to be at Release 3 genomic coordinate 9308219 on arm 3L *F and the insertion site of PBac{RB}e04497 to be at Release 3 genomic *F coordinate 9381019 on arm 3L. The cytological breakpoints of Df(3L)BSC113 *F predicted from these coordinates are 67A8;67B4. It failed to complement *F P{PZ}eIF-4E07238 and P{lacW}aayS042314. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188656 *a Deal *b J. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC114. *F Date: Fri, 22 Jul 2005 14:51:24 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC114 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(2L)BSC114 *F Jennifer Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC114 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d11137 and PBac{WH}f03244. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F P{XP}d11137/PBac{WH}f03244 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC114 from the segment *F of P{XP}d11137 to the left of its FRT site and the segment of *F PBac{WH}f03244 to the right of its FRT site. Its presence was verified *F using the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of P{XP}d11137 to be at Release 3 genomic *F coordinate 21732458 on chromosome arm 2L and the insertion site of *F PBac{WH}f03244 to be at Release 3 genomic coordinate 21748327 on arm *F 2L. The Gene Disruption Project determined the insertion site of *F P{XP}d11137 to be at Release 3 genomic coordinate 21732520 on arm 2L and *F the insertion site of PBac{WH}f03244 to be at Release 3 genomic coordinate *F 21748325 on arm 2L. The cytological breakpoints of Df(2L)BSC114 predicted *F from these coordinates are 40B3;40B4. Deletion homozygotes are viable, but *F PCR failed to amplify 588 bp and 810 bp segments lying between P{XP}d11137 *F and PBac{WH}f03244 from the homozygotes using the primer pairs *F 5'-TGCGGACTGCTGTTTTTGCT-3'/5'-CTTTGTTGGTCTGTTTGTTGTG-3' and *F 5'-CAACACTCGCAATCATTTGTG-3'/5'-AGTCGTAAACGCTTCCTTCTGCCC-3', respectively. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188657 *a Deal *b J. *c M. *d Deal *e K. *f Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(2L)BSC115. *F Date: Fri, 22 Jul 2005 14:51:25 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2L)BSC115 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(2L)BSC115 *F Jennifer Deal, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2L)BSC115 was isolated as a FLP recombinase-induced recombination event *F involving P{XP}d11137 and PBac{WH}f02470. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; *F P{XP}d11137/PBac{WH}f02470 males crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO females. These males were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC115 from the segment *F of P{XP}d11137 to the left of its FRT site and the segment of *F PBac{WH}f02470 to the right of its FRT site. Its presence was verified *F using the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of P{XP}d11137 to be at Release 3 genomic *F coordinate 21732458 on chromosome arm 2L and the insertion site of *F PBac{WH}f02470 to be at Release 3 genomic coordinate 21757506 on arm *F 2L. The Gene Disruption Project determined the insertion site of *F P{XP}d11137 to be at Release 3 genomic coordinate 21732520 on arm 2L and *F the insertion site of PBac{WH}f02470 to be at Release 3 genomic coordinate *F 21757504 on arm 2L. The cytological breakpoints of Df(2L)BSC115 predicted *F from these coordinates are 40B3;40B5. Deletion homozygotes are viable, but *F PCR failed to amplify 588 bp and 810 bp segments lying between P{XP}d11137 *F and PBac{WH}f03244 from the homozygotes using the primer pairs *F 5'-TGCGGACTGCTGTTTTTGCT-3'/5'-CTTTGTTGGTCTGTTTGTTGTG-3' and *F 5'-CAACACTCGCAATCATTTGTG-3'/5'-AGTCGTAAACGCTTCCTTCTGCCC-3', respectively. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188658 *a Andrade *b R. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC116. *F Date: Fri, 22 Jul 2005 14:52:30 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC116 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(3L)BSC116 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC116 was isolated as a FLP recombinase-induced recombination event *F involving PBac{RB}e00847 and P{XP}d06681. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; *F PBac{RB}e00847 /P{XP}d06681 females crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO males. These females were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.RB3}BSC116 from the segment *F of PBac{RB}e00847 to the left of its FRT site and the segment of *F P{XP}d06681 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al with the substitution *F of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' *F minus primer in the Hybrid PCR protocol in the Supplementary *F Methods. Exelixis, Inc. determined the insertion site of PBac{RB}e00847 to *F be at Release 3 genomic coordinate 2354239 on chromosome arm 3L and the *F insertion site of P{XP}d06681 to be at Release 3 genomic coordinate 2534408 *F on arm 3L. The Gene Disruption Project determined the insertion site of *F PBac{RB}e00847 to be at Release 3 genomic coordinate 2354423 on arm 3L and *F the insertion site of P{XP}d06681 to be at Release 3 genomic coordinate *F 2534411 on arm 3L. The cytological breakpoints of Df(3L)BSC116 predicted *F from these coordinates are 62D7;62E6; however, our polytene chromosome *F squashes showed the breakpoints 62E1-2;62E5-6. (The deletion forms a *F fusion band composed of part of the 62E1,2 doublet and all or part of *F 62E6.) Df(3L)BSC116 failed to complement Df(3L)Aprt-32 and Df(3L)R-G7. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188659 *a Andrade *b R. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC118. *F Date: Fri, 22 Jul 2005 14:53:29 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC118 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(3L)BSC118 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC118 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f05665 and P{XP}d01848. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; *F PBac{WH}f05665/P{XP}d01848 females crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO males. These females were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC118 from the segment *F of PBac{WH}f05665 to the left of its FRT site and the segment of *F P{XP}d01848 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f05665 to be at Release 3 genomic *F coordinate 9473058 on chromosome arm 3L and the insertion site of *F P{XP}d01848 to be at Release 3 genomic coordinate 9654724 on arm 3L. The *F Gene Disruption Project determined the insertion site of PBac{WH}f05665 to *F be at Release 3 genomic coordinate 9473201 on arm 3L and the insertion site *F of P{XP}d01848 to be at Release 3 genomic coordinate 9654720 on arm *F 3L. The cytological breakpoints of Df(3L)BSC118 predicted from these *F coordinates are 67B10;67C5. It failed to complement Df(3L)Exel6114, *F P{PZ}fry02240 and alphaTub67C1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188660 *a Andrade *b R. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC117. *F Date: Fri, 22 Jul 2005 14:52:30 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC117 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(3L)BSC117 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC117 was isolated as a FLP recombinase-induced recombination event *F involving PBac{RB}e04444 and P{XP}d02813. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; *F PBac{RB}e04444 /P{XP}d02813 females crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO males. These females were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.RB3}BSC117 from the segment *F of PBac{RB}e04444 to the left of its FRT site and the segment of *F P{XP}d02813 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al with the substitution *F of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' *F minus primer in the Hybrid PCR protocol in the Supplementary *F Methods. Exelixis, Inc. determined the insertion site of PBac{RB}e04444 to *F be at Release 3 genomic coordinate 7208899 on chromosome arm 3L and the *F insertion site of P{XP}d02813 to be at Release 3 genomic coordinate 7294608 *F on arm 3L. The Gene Disruption Project determined the insertion site of *F PBac{RB}e04444 to be at Release 3 genomic coordinate 7209004 on arm 3L and *F the insertion site of P{XP}d02813 to be at Release 3 genomic coordinate *F 7294515 on arm 3L. The cytological breakpoints of Df(3L)BSC117 predicted *F from these coordinates are 65F2;65F5; however our polytene chromosome *F squashes showed the breakpoints 65E9-F1;65F2-5 (the 65E8,9 doublet band and *F 65F5 are present; the 65F1,2 doublet band is absent). Df(3L)BSC117 failed *F to complement Df(3L)BCS33. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188661 *a Andrade *b R. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC119. *F Date: Fri, 22 Jul 2005 14:53:30 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC119 *F Cc: Rachel Andrade , jedeal@bio.indiana.edu, *F medeal@bio.indiana.edu, kaufman@bio.indiana.edu *F Isolation and characterization of Df(3L)BSC119 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC119 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f01070 and P{XP}d07033. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; *F PBac{WH}f01070/P{XP}d07033 females crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO males. These females were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC119 from the segment *F of PBac{WH}f01070 to the left of its FRT site and the segment of *F P{XP}d07033 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f01070 to be at Release 3 genomic *F coordinate 2580837 on chromosome arm 3L and the insertion site of *F P{XP}d07033 to be at Release 3 genomic coordinate 2804237 on arm 3L. The *F Gene Disruption Project determined the insertion site of PBac{WH}f01070 to *F be at Release 3 genomic coordinate 2580912 on arm 3L and the insertion site *F of P{XP}d07033 to be at Release 3 genomic coordinate 2804243 on arm *F 3L. The cytological breakpoints of Df(3L)BSC119 predicted from these *F coordinates are 62E7;62F5. Polytene chromosome squashes verified the *F presence of a deletion. Df(3L)BSC119 failed to complement Df(3L)BSC23. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188662 *a Andrade *b R. *c K. *d Cook *t 2005.7.22 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC120. *F Date: Fri, 22 Jul 2005 16:07:37 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC120 *F Cc: Rachel Andrade , medeal@bio.indiana.edu, *F jedeal@bio.indiana.edu, Stacey Christensen *F Isolation and characterization of Df(3L)BSC120 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC120 was isolated as a FLP recombinase-induced recombination event *F involving PBac{WH}f04976 and P{XP}d03723. The deletion was isolated as a *F chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; *F PBac{WH}f04976/P{XP}d03723 females crossed to w1118; P{w+mC=hs-hid}2, *F wgSp-1/CyO males. These females were heat shocked as larvae as described *F in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This *F cross and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks (Thibault et *F al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination *F event generated the genetic element P+PBac{XP5.WH5}BSC120 from the segment *F of PBac{WH}f04976 to the left of its FRT site and the segment of *F P{XP}d03723 to the right of its FRT site. Its presence was verified using *F the PCR methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of PBac{WH}f04976 to be at Release 3 genomic *F coordinate 11863246 on chromosome arm 3L and the insertion site of *F P{XP}d03723 to be at Release 3 genomic coordinate 12039353 on arm 3L. The *F Gene Disruption Project determined the insertion site of PBac{WH}f04976 to *F be at Release 3 genomic coordinate 11863241 on arm 3L and the insertion *F site of P{XP}d03723 to be at Release 3 genomic coordinate 12039369 on arm *F 3L. The cytological breakpoints of Df(3L)BSC120 predicted from these *F coordinates are 68E3;68F2; however, our polytene chromosome squashes showed *F the breakpoints 68E3-F1;68F3-5 (68E3 and the 68F5,6 doublet band are *F present; the 68F1,2 doublet and 68F3 are absent). It failed to complement *F Df(3L)Exel6115 and Df(3L)vin7. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188663 *a Nusse *b R. *c S. *d Crews *e T. *f Ip *g P. *h Lawrence *i L.S. *j Shashidhara *t 2005.8.1 *T personal communication to FlyBase *u Wnt8/wntD nomenclature. *F Date: Thu, 21 Jul 2005 15:44:35 \-0700 *F Subject: Nomenclature *F From: Roel Nusse *F To: , Peter Lawrence , *F *F CC: 'Rachel Drysdale (Genetics)' , Michael Gordon *F *F Dear colleagues, *F I am writing to you about a nomenclature issue. *F We have been working on a Drosophila gene CG8458 , also called Wnt8. It is a *F distant member of the Wnt family, and not the Drosophila ortholog of the *F vertebrate Wnt8 gene. We have generated a loss of function mutant and found *F a phenotype indicating that this Wnt antagonizes Dorsal in Drosophila. We *F intend therefore to term the gene wntD (wnt inhibitor of Dorsal). This name *F reflects the function (which we describe for the first time), and the Wnt *F homology and does not suggest orthology with any other Wnt. In the past, I *F have been trying to keep the Wnt nomenclature such that numbers would *F reflect an orthologous relationship (see the Wnt homepage, *F http://www.stanford.edu/~rnusse/wntwindow.html) *F The group of Tony Ip agrees with us and they have recently published a paper *F on line in Development using the same nomenclature (Ganguly, A., Jiang, J., *F and Ip, Y. T. (2005). Drosophila WntD is a target and an inhibitor of the *F Dorsal/Twist/Snail network in the gastrulating embryo. *F We are now writing a paper. To get the nomenclature issue straightened out, *F I contacted Rachel Drysdale. She does agree with the wntD name, after *F consulting Kevin Cook, of the Nomenclature Committee, and Michael Ashburner. *F However, she asked me to contact everyone who has published on the gene to *F see whether they agree to use the wntD name. *F This is what I a now doing. I hope to hear from you soon. *F \------------------------------------- *F Roel Nusse *F Howard Hughes Medical Institute *F Dept. of Developmental Biology *F Stanford University, School of Medicine *F Beckman Center, B271 *F 279 Campus Drive *F Stanford, CA 94305-5323 *F 650-723-7769 *F Fax 650-723-1399 *F http://www.stanford.edu/~rnusse/wntwindow.html *F ============================================================================ *F From: 'L S Shashidhara' *F To: 'Roel Nusse' , , 'Peter *F Lawrence' *F Cc: 'Rachel Drysdale \(Genetics\)' , 'Michael Gordon' *F , *F Subject: Re: Nomenclature *F Date: Fri, 22 Jul 2005 08:31:13 \+0530 *F Dear Roel, *F I agree with the new nomenclature for Wnt8 (new name WntD). *F regards *F shashi *F \------------------------------------------------------------- *F L S Shashidhara Ph.D. *F Centre for Cellular and Molecular Biology *F Uppal Road, Hyderabad 500 007, INDIA *F E-mail: shashi@ccmb.res.in *F Web site: http://www.ccmb.res.in/staff/shashi/droso.html *F http://www.ccmb.res.in/staff/shashi/publication.html *F \------------------------------------------------------------- *F ============================================================================ *F Date: Mon, 1 Aug 2005 12:27:05 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Peter Lawrence *F Subject: Re: Nomenclature *F THANKS FOR WRITING,SORRY I HAVE BEEN IN REMOTE SWEDISH WOODLAND. *F ANYWAY YOUR PLAN SEEMS SENSIBLE TO ME, ALL THE BEST, PETER *F \-- *F MRC Laboratory of Molecular Biology *F Hills Rd., *F Cambridge CB2 2QH *F UK *F Tel. lab 44 (0) 1223 402282 *F office 1223 402226 *F fax 1223 411582 *F ============================================================================ *F From: 'Stephen Crews' *F Date: July 22, 2005 12:07:56 PM PDT *F To: ''Roel Nusse'' *F Subject: RE: Nomenclature *F Reply-To: *F Roel, *F Change of name to wntD is fine with my lab. *F Steve *F \------------------------------------------------ *F Stephen Crews *F Professor *F The University of North Carolina at Chapel Hill *F Department of Biochemistry/PMBB *F CB#3280 Fordham Hall *F Chapel Hill, NC 27599-3280 *F (919) 962-4380 (Tel) *F (919) 962-8472 (Fax) *F steve_crews@unc.edu *F http://www.unc.edu/~crews *F ============================================================================ *F >From fbserver@rail.bio.indiana.edu Sun Jul 24 19:03:50 2005 *F Date: Sun, 24 Jul 2005 13:04:54 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F Reply-to: Tony.Ip@umassmed.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 057 Gene data (problem or question) *F comments: We recently published a paper in Development *F Ganguly A, Jiang J, Ip YT. Drosophila WntD is a target and an inhibitor of *F the Dorsal/Twist/Snail network in the gastrulating embryo. Development. 2005 *F Aug;132(15):3419-29. Epub 2005 Jun 29. *F describing using the gene name wntD (lower case) to replace Wnt8 (CG8458). *F The protein name should be WntD, stands for Wnt inhibitor of Dorsal. This was *F agreed between our lab and Dr. Roel Nusse's lab at Stanford University (see *F acknowledgement in the paper). They are in the process of publishing their *F results. Please update this information in the flybase when appropriate. *F realname: Tony Ip *F reply-to: Tony.Ip@umassmed.edu *F source: FB Help Mail: *F Sent from computer 146.189.74.128 *F ============================================================================ # *U FBrf0188664 *a Baeg *b G.H. *t 2005.8.8 *T personal communication to FlyBase *u P{UAS-dlp.WT}3. *F Date: Mon, 08 Aug 2005 10:18:31 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-dlp.WT}3 *F The following information accompanied a stock donated to the *F Bloomington Stock Center by Gyeong-Hun Baeg, Harvard Medical School (8/05). *F P{UAS-dlp.WT}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188665 *a Wilhelm *b J. *t 2005.8.23 *T personal communication to FlyBase *u CG10686 is Trailer Hitch. *F Date: Tue, 23 Aug 2005 13:37:35 \-0500 (EST) *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 016 CG10686 is Trailer Hitch *F comments: Dear Curator, *F I just wanted to update you that the gene that we have previously called *F trailer hitch is CG10686. We have abbreviated it tral due to all the other *F obvious abreviations having already been taken. *F Sincerely, *F Jim Wilhelm *F realname: Jim Wilhelm *F reply-to: wilhelm@ciwemb.edu *F source: FB Help Mail: *F usersubject: CG10686 is Trailer Hitch *F Sent from computer 192.124.118.129 # *U FBrf0188666 *a Mazzalupo *b S. *t 2005.8.23 *T personal communication to FlyBase *u database correction. *F To: flybase-updates@morgan.harvard.edu *F From: Stacy Mazzalupo *F Subject: correction *F Date: Tue, 23 Aug 2005 18:17:18 \-0700 *F To Whom It May Concern: *F There is a listing of Dmer\sly1 in FBal0150893 *F This is a misreading of the original stock list. The allele is sly *F (for spotless yellow). *F This is the original spotless reference: *F Title: GENETIC SYSTEM IN PARTHENOGENETIC STRAINS OF *F DROSOPHILA-MERCATORUM *F Author(s): CARSON HL *F Source: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED *F STATES OF AMERICA 70 (6): 1772-1774 1973 *F This reference says that yellow and spotless are tightly linked: *F Title: THE MOLECULAR THROUGH ECOLOGICAL GENETICS OF ABNORMAL *F ABDOMEN IN DROSOPHILA MERCATORUM. I. BASIC GENETICS *F Author(s): ALAN R. TEMPLETON, TERESA J. CREASE AND FAITH SHAH *F Source: Genetics 1985 111: 805-818 *F Thank you to Robert Eisman, for finding the Carson reference for me. *F Stacy *F To: flybase-updates@morgan.harvard.edu *F From: Stacy Mazzalupo *F Subject: new allele *F Date: Tue, 23 Aug 2005 16:46:04 \-0700 *F To Whom it May Concern: *F I am unsure if this is an allele, an aberration or something else. *F However, we have a stock *F that is parthenogenetic (a.k.a. impaternate). We call it im1. Could *F an FBal (or whatever *F is most appropriate) be created for Dana\im1? *F This stock is described in reference FBrf0179330. *F Thanks, *F Stacy *F To: flybase-updates@morgan.harvard.edu *F From: Stacy Mazzalupo *F Subject: database correction *F Date: Tue, 23 Aug 2005 16:39:43 \-0700 *F To Whom it May Concern: *F The FBal0053388 has a misspelling. *F It should be curled-Saito *F Thank you, *F Stacy *F Stacy Mazzalupo, PhD *F Assistant Staff Scientist *F Manager, Tucson Stock Center *F University of Arizona *F e-mail: smm@email.arizona.edu *F phone: (520) 626-6322 *F fax: (520) 626-5863 # *U FBrf0188667 *a Cooley *b L. *t 2005.8.22 *T personal communication to FlyBase *u UAS-Actin-GFP constructs. *F Date: Tue, 23 Aug 2005 10:45:37 \-0500 *F To: 'Rachel Drysdale (Genetics)' *F From: Kevin Cook *F Subject: Re: Two different UAS-Actin-GFP constructs *F Cc: matthewk@indiana.edu, Lynn Cooley *F Rachel-- *F ..... *F Lynn wrote yesterday requesting that we simplify *F the insertion names while we're changing the *F construct name. So, let's do the following: *F Change P{UAS-Act5C.T:GFP}127.1.1 (FBti0038021) to P{UASp-Act5C.T:GFP}1 *F Change P{UAS-Act5C.T:GFP}127.37.2 (FBti0038022) to P{UASp-Act5C.T:GFP}2 *F Change P{UAS-Act5C.T:GFP}127.18.4 (FBti0038023) to P{UASp-Act5C.T:GFP}3 *F And, of course, keep the old 127etc names as synonyms. *F ..... *F Kevin *F P.S. Here's Lynn's note: *F Kevin, *F Andy and I are for changing the whole name now instead of going half way. *F So the changes would be: *F P{UAS-Act5C.T:GFP}127.37.2 to P{UASp-Act5C.GFP}2 *F P{UAS-Act5C.T:GFP}127.18.4 to P{UASp-Act5C.GFP}3 *F The second change would apply to your stock number 7312, too. I like it that *F the old names will stay in synonyms. *F Thanks for your help, *F Lynn # *U FBrf0188668 *a Andrade *b R. *c K. *d Cook *t 2005.8.25 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC121. *F Date: Thu, 25 Aug 2005 16:08:02 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC121 *F Isolation and characterization of Df(3L)BSC121 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC121 was isolated as a FLP recombinase-induced recombination *F event involving P{XP}d10431 and PBac{RB}e00237. The deletion was *F isolated as a chromosome lacking miniwhite markers in progeny of *F P{hsFLP}1, w1118; P{XP}d10431/PBac{RB}e00237 females crossed to *F w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were *F heat shocked as larvae as described in Parks et al., Nature Genetics *F 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding *F and succeeding generations maintained the original genetic background *F of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: *F 283-287, 2004; FBrf0175002). The recombination event generated the *F genetic element P+PBac{XP5.RB3}BSC121 from the segment of P{XP}d10431 *F to the left of its FRT site and the segment of PBac{RB}e00237 to the *F right of its FRT site. Its presence was verified using the PCR *F methods and primers described in Parks et al. with the substitution *F of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or *F RB3' minus primer in the Hybrid PCR protocol in the Supplementary *F Methods. Exelixis, Inc. determined the insertion site of P{XP}d10431 *F to be at Release 3 genomic coordinate 160641 on chromosome arm 3L and *F the insertion site of PBac{RB}e00237to be at Release 3 genomic *F coordinate 184051 on arm 3L. The Gene Disruption Project determined *F the insertion site of P{XP}d10431 to be at Release 3 genomic *F coordinate 160825 on arm 3L and the insertion site of PBac{RB}e00237 *F to be at Release 3 genomic coordinate 184299 on arm 3L. The *F cytological breakpoints of Df(3L)BSC121 predicted from these *F coordinates are 61B2;61B2. It failed to complement Df(3L)Exel6084 *F and Df(3L)ED201, but complemented Df(3L)Exel6083 and Df(3L)ED4079. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188669 *a Andrade *b R. *c K. *d Cook *t 2005.8.25 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC123. *F Date: Thu, 25 Aug 2005 16:08:10 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC123 *F Isolation and characterization of Df(3L)BSC123 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC123 was isolated as a FLP recombinase-induced recombination *F event involving P{XP}d03930 and PBac{WH}f05230. The deletion was *F isolated as a chromosome lacking miniwhite markers in progeny of *F P{hsFLP}1, w1118; P{XP}d03930/PBac{WH}f05230 females crossed to *F w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were *F heat shocked as larvae as described in Parks et al., Nature Genetics *F 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding *F and succeeding generations maintained the original genetic background *F of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: *F 283-287, 2004; FBrf0175002). The recombination event generated the *F genetic element P+PBac{XP5.WH5}BSC123 from the segment of P{XP}d03930 *F to the left of its FRT site and the segment of PBac{WH}f05230 to the *F right of its FRT site. Its presence was verified using the PCR *F methods and primers described in Parks et al. Exelixis, Inc. *F determined the insertion site of P{XP}d03930 to be at Release 3 *F genomic coordinate 1775286 on chromosome arm 3L and the insertion *F site of PBac{WH}f05230 to be at Release 3 genomic coordinate 1786783 *F on arm 3L. The Gene Disruption Project determined the insertion site *F of P{XP}d03930 to be at Release 3 genomic coordinate 1775476 on arm *F 3L and the insertion site of PBac{WH}f05230 to be at Release 3 *F genomic coordinate 1786781 on arm 3L. The cytological breakpoints of *F Df(3L)BSC123 predicted from these coordinates are 62B4;62B4. It *F failed to complement Cdc37e4D and Df(3L)Exel6088. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188670 *a Andrade *b R. *c K. *d Cook *t 2005.8.25 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC122. *F Date: Thu, 25 Aug 2005 16:08:08 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC122 *F Isolation and characterization of Df(3L)BSC122 *F Rachel Andrade and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC122 was isolated as a FLP recombinase-induced recombination *F event involving PBac{RB}e01500 and P{XP}d06594. *F The deletion was *F isolated as a chromosome lacking miniwhite markers in progeny of *F P{hsFLP}1, w1118; PBac{RB}e01500/P{XP}d06594 females crossed to *F w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were *F heat shocked as larvae as described in Parks et al., Nature Genetics *F 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding *F and succeeding generations maintained the original genetic background *F of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: *F 283-287, 2004; FBrf0175002). The recombination event generated the *F genetic element P+PBac{XP5.RB3}BSC122 from the segment of *F PBac{RB}e01500 to the left of its FRT site and the segment of *F P{XP}d06594 to the right of its FRT site. Its presence was verified *F using the PCR methods and primers described in Parks et al. with the *F substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the *F RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the *F Supplementary Methods. Exelixis, Inc. determined the insertion site *F of PBac{RB}e01500 to be at Release 3 genomic coordinate 1737276 on *F chromosome arm 3L and the insertion site of P{XP}d06594 to be at *F Release 3 genomic coordinate 1774835 on arm 3L. The Gene Disruption *F Project determined the insertion site of PBac{rB}e01500 to be at *F Release 3 genomic coordinate 1737279 on arm 3L and the insertion site *F of P{XP}d06594 to be at Release 3 genomic coordinate 1774992 on arm *F 3L. The cytological breakpoints of Df(3L)BSC122 predicted from these *F coordinates are 62B1;62B4. It failed to complement P{PZ}dlt04276, *F Df(3L)Exel6088 and Df(3L)Aprt-1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188671 *a Fehon *b R. *t 2005.8.26 *T personal communication to FlyBase *u P{cosMer+}3 insertion. *F Date: Fri, 26 Aug 2005 09:25:50 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{cosMer+}3 insertion *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Rick Fehon, University of Chicago (7/05). *F P{cosMer+}3 is a third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188672 *a Stacy Mazzalupo *b ?. *t 2005.8.24 *T personal communication to FlyBase *u *F To: flybase-updates@morgan.harvard.edu *F From: Stacy Mazzalupo *F Subject: new allele *F Date: Wed, 24 Aug 2005 10:49:08 \-0700 *F Rachel: *F 2 more new alleles: *F Please can you create a new FBal for Dari\w1 (white gene)? *F This mutation arose in an isofemale line that was collected from *F Peralta Canyon *F in the Superstition Mountains of Arizona. (Sergio Castrezana, 04/1997) *F This white-eyed mutant was found and donated by Brook L'Flamme *F (05/2005). *F These flies have white eyes, but no additional pigmentation phenotype. *F The trait is X-linked recessive. *F There is also low fecundity in the phenotypic flies. *F Please can you create a new FBal for Drep\cd1 (cardinal gene)? *F This mutation arose spontaneously in an isofemale line that was *F collected from a Pulque bar *F near Cholula Pyramid (Puebla, Mexico) in 2002 by Sergio Castrezana. *F This mutation was identified by Sergio Catrezana and donated to our *F collection on 3/10/2003. *F The flies have bright red eyes, but no additional pigmentation *F phenotype. *F The trait is recessive. *F Thank you, *F Stacy *F Stacy Mazzalupo, PhD *F Assistant Staff Scientist *F Manager, Tucson Stock Center *F University of Arizona *F e-mail: smm@email.arizona.edu *F phone: (520) 626-6322 *F fax: (520) 626-5863 # *U FBrf0188673 *a Green *b M. *t 2005.9.1 *T personal communication to FlyBase *u Dp(1;3)wm76. *F Date: Thu, 01 Sep 2005 11:55:59 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Dp(1;3)wm76 *F The following information accompanied a stock donated to the *F Bloomington Stock Center by Mel Green, University of California at Davis. *F Dp(1;3)wm76 is a small duplication isolated in 1976 by George *F Lefevre, Jr. It rescues w and crm mutations, but the breakpoints are *F otherwise unknown. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188674 *a Bloomington Drosophila Stock Center *b ?. *t 2005.8.31 *T personal communication to FlyBase *u P{UAS-Lar.K}P4B. *F Date: Wed, 31 Aug 2005 15:04:38 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Lar.K}P4B *F P{UAS-Lar.K}P4B is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188675 *a Thomas *b B. *t 2005.8 *T personal communication to FlyBase *u swm. *F Date: Tue, 30 Aug 2005 14:00:35 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: swm *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Barbara Thomas, National Cancer *F Institute, National Institutes of Health (8/05) *F The locus l(2)37Dh has been renamed 'second mitotic wave missing' *F (swm). It corresponds to CG10084 and was referred to as S(rux)2B in *F Dong et al., 1997 (FBrf0091048). *F l(2)37Dh5 has been renamed swm37Dh-5. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188676 *a Thomas *b B. *t 2005.8 *T personal communication to FlyBase *u Thomas insertions & Rca1IX. *F Date: Tue, 30 Aug 2005 13:48:51 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Thomas insertions & Rca1IX *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Barbara Thomas, National Cancer *F Institute, National Institutes of Health (8/05) *F P{hs-rca1}I4 is a homozygous viable and fertile, third chromosome insertion. *F P{hs-rca1}C2 is a homozygous viable and fertile, second chromosome insertion. *F P{UAS-rux.T}C1 is a second chromosome insertion. *F Rca1IX is a strong loss-of-function allele of Rca1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188677 *a Ng *b J. *t 2005.7 *T personal communication to FlyBase *u ssh1-63. *F Date: Tue, 30 Aug 2005 10:14:44 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: ssh1-63 *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Julian Ng, MRC Centre for Developmental *F Neurobiology, King's College (7/05). *F ssh1-63 is a null allele of ssh generated by imprecise excision of *F P{PZ}ssh01207. The screen generating this allele was described in *F Niwa et al., Cell 108(2):233-246, 2002 (FBrf0144752). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188678 *a Matusek *b T. *t 2005.9.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-CB22. *F Date: Tue, 6 Sep 2005 10:34:43 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-CB22 *F To: matusek@brc.hu *F Cc: rd120@gen.cam.ac.uk *F Dear Tamas, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F The novel Drosophila formin, dDAAM works together with src kinases to *F regulate tissue specific actin structures. *F You mention a gene symbol that is new to FlyBase, DAAM. Do you know which of *F the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. The CG symbols become synonyms when an annotation is *F named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F From: 'Matusek' *F To: 'Rachel Drysdale (Genetics)' *F Date: Tue, 06 Sep 2005 13:03:27 \+0200 *F Dear Rachel, *F The dDAAM gene corresponds to the CG14622 annotation located *F on the tip of the X chromosome in the cytological region 1F2-3. This *F gene is the Drosophila homolog of the recently described Daam1 *F (Cell. 2001 Dec 28;107(7):843-54). *F Best, *F Tamas # *U FBrf0188679 *a Maier *b D. *t 2005.9.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-SG14. *F Date: Tue, 6 Sep 2005 10:31:10 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-SG14 *F To: maierdie@uni-hohenheim.de *F Cc: rd120@gen.cam.ac.uk *F Hi Dieter, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Subcellular expression of Drosophila PKD conforms to a role in the *F regulation of secretory protein transport. *F You mention a gene symbol that is new to FlyBase, PKD. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Tue, 06 Sep 2005 13:24:12 \+0200 *F Subject: Re: Helping FlyBase: EDRC-SG14 *F From: 'Dieter Maier' *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F the PKD has the flybase annotation number CG7125. PKD was originally *F classified as PKCm (human) or PKD1 (mouse). In vertebrates there are three *F PKD known (PKD1 (PKCm), PKD2, PKD3 (PKCn). These genes has been in first *F studies classified as new PKC's, however, the investigators found better *F relationship to CAMP's. Therefore they called it PKD's (Protein Kinase D). *F For a review see Rykx et al. 2003 FEBS Letters 546, 81-86. *F I hope I could help you. *F With best regards *F Dieter *F \---------------------------------------------------------------------------- *F Dieter Maier *F University Hohenheim *F Genetics (240) *F Garbenstr, 30 *F 70599 Stuttgart *F Germany *F Tel.: 0711 459 2215 *F Fax : 0711 459 2211 *F email: maierdie@uni-hohenheim.de *F \---------- # *U FBrf0188680 *a White-Cooper *b H. *t 2005.9.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-GG6. *F Date: Tue, 6 Sep 2005 10:34:23 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-GG6 *F To: helen.white-cooper@zoo.ox.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Helen, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Gene expression in Drosophila testes. *F You mention a gene symbol that is new to FlyBase, tomb. Do you know which of *F the Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. The CG symbols become synonyms when an annotation is *F named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Tue, 6 Sep 2005 12:54:08 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Helen White-Cooper *F Subject: Re: Helping FlyBase: EDRC-GG6 *F It is CG14016. We called it tombola (tomb) because each homozygous *F mutant testis looks like a tube full of balls. *F Helen *F PS, I noticed that the gene symbol zab never got incorporated as a *F synonym of comr. zab had been our working name for this mutant, and *F was mentioned in abstracts, but we renamed it comr when we published. *F \------------------------------------------------------------------------------ *F -- *F Helen White-Cooper *F Dept of Zoology *F University of Oxford *F South Parks Road *F Oxford *F OX1 3PS *F Tel: \+44 (0)1865 271195 (office) 271291 (lab) *F Fax: \+44 (0)1865 310447 # *U FBrf0188681 *a Lada *b K. *t 2005.9.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-CC11. *F Date: Tue, 6 Sep 2005 10:30:24 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-CC11 *F To: k.lada@gen.cam.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Karolina, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Meteor, a Novel Nuclear-Membrane Associated Protein Required for Nuclear *F Integrity and Polo kinase recruitment to the Nuclear Envelope. *F You mention a gene symbol that is new to FlyBase, meteor. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F From: 'Karolina Lada' *F To: 'Rachel Drysdale \(Genetics\)' *F Subject: Re: Helping FlyBase: EDRC-CC11 *F Date: Tue, 6 Sep 2005 13:29:54 \+0100 *F Dear Rachel, *F The gene which I study is CG14066 and has previously been published as larp *F (ie. already exists in your database under this name). *F . *F . *F Kind regards, *F Karolina # *U FBrf0188682 *a Deak *b P. *t 2005.9.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-CC13. *F Date: Tue, 6 Sep 2005 10:29:59 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-CC13 *F To: palm@brc.hu *F Cc: rd120@gen.cam.ac.uk *F Dear Margit, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Genetic analysis of the anaphase promoting complex in Drosophila *F melanogaster. *F You mention gene symbols that are new to FlyBase, APC4, APC7 and *F cdc23. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F From: 'Deak Peter' *F To: 'Rachel Drysdale (Genetics)' *F Date: Tue, 06 Sep 2005 17:11:39 \+0200 *F Subject: Helping FlyBase: EDRC-CC13 *F Dear Rachel, *F Margit passed your message to me. *F You mention gene symbols that are new to FlyBase, APC4, APC7 and *F cdc23. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what *F is actually the same gene unless we can't avoid it. The CG symbols *F become synonyms when an annotation is named with a more descriptive or *F functional name. *F APC4 corresponds to CG32707 (previously it was known as CG4350). We *F found that its sequence shows significant homology to yeast and human *F APC4. Based on this sequence similarity, we consider it as the Drosophila *F APC4 ortholog. *F APC7 corresponds to CG14444. It again shows similarity to *F human APC7, therefore it should be considered as its Drosophila ortholog. *F Silencing this gene by transgenic RNAi causes characteristic mitotic *F phenotype that doesn't affect the viability and fertility of those flies. *F It interacts genetically with other APC subunits. *F Cdc23 corresponds to CG2508. It shows sequence homology to yeast Cdc23 *F and to human APC8. Best wishes, *F Peter Deak # *U FBrf0188683 *a Grosshans *b J. *t 2005.9.6 *T personal communication to FlyBase *u Helping FlyBase: EDRC-CB2. *F Date: Tue, 6 Sep 2005 10:35:49 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-CB2 *F To: j.grosshans@zmbh.uni-heidelberg.de *F Cc: rd120@gen.cam.ac.uk *F Dear Jorg, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Developmental control of nuclear size and shape by kugelkern and *F kurzkern. *F You mention gene symbols that are new to FlyBase, kugelkern and *F kurzkern. Do you know which of the Genome Project CG annotations your *F genes correspond to? All the CGs have corresponding gene records in *F FlyBase already and we don't like to make duplicate records for what is *F actually the same gene unless we can't avoid it. The CG symbols become *F synonyms when an annotation is named with a more descriptive or *F functional name. *F Also, if kugelkern and/or kurzkern have picked up a short symbol since *F you submitted the abstract then this would be a good time to tell me so *F I can get it/them into the database. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Tue, 06 Sep 2005 19:52:51 \+0200 *F Subject: Re: Helping FlyBase: EDRC-CB2 *F From: Jörg Großhans *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F the CG number for kugelkern (short symbol: kug) is CG5175. We have not *F definetely identified kurzkern (short symbol: kur), but mapped it to 2B;3C *F by complementation with duplications. *F We have a paper in submission/revision so that there will be hopefully a *F full reference soon. *F Please let me know if you need further information. Thanks a lot for your *F efforts. *F Best regards, Jörg *F \----------------------------------------------- *F Jörg Großhans *F ZMBH, Uni Heidelberg *F Im Neuenheimer Feld 282 *F 69120 Heidelberg *F e-mail: j.grosshans@zmbh.uni-heidelberg.de *F www: http://www.zmbh.uni-heidelberg.de *F Tel.: \+49 (6221) 546884 *F Fax.: \+49 (6221) 545892 *F \------------------------------------------------ *F Date: Thu, 08 Sep 2005 13:37:02 \+0200 *F Subject: Re: Helping FlyBase: EDRC-CB2 *F From: Jörg Großhans *F To: 'Rachel Drysdale (Genetics)' *F CC: Annely Haase *F Dear Rachel, *F I am very sorry for the confusion. Let's use kuk. *F Thank you very much for your efforts, *F best wishes, Jörg *F >> the CG number for kugelkern (short symbol: kug) is CG5175. *F > *F > we have a slight problem in that kug is already taken as a symbol for *F > kugelei (FBgn0001329). This gene also has a synonym 'kugel'. *F > *F > In order to avoid confusion, might I suggest you go with kuk, or kugk, *F > for kugelkern \- these alternatives are virtually the same for *F > pronunciation, and will avoid confusion with FBgn0001329. *F > *F > Let me know which you prefer and I will make it so. *F > *F > My best wishes, *F > *F > Rachel. # *U FBrf0188684 *a Lasko *b P. *t 2005.9.7 *T personal communication to FlyBase *u Helping FlyBase: EDRC-PF2. *F Date: Tue, 6 Sep 2005 10:36:29 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-PF2 *F To: Paul.Lasko@mcgill.ca *F Cc: rd120@gen.cam.ac.uk *F Hi Paul, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Inhibition of caudal translation by Bicoid via 5'-3' tethering of the *F mRNA by an eIF4E-cognate protein, d4EHP. *F Thing is, there are two genes with '4EHP' as synoym in FlyBase \- *F CG8023, CG33100. Is your 'd4EHP' one of these? *F My best regards, *F Hope everything going swimmingly for you and yours. *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Wed, 07 Sep 2005 10:52:48 \-0400 *F Subject: Re: Helping FlyBase: EDRC-PF2 *F From: Paul Lasko *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F 4EHP is CG33100. *F Things are going swimmingly indeed--and with you? *F Kind regards, Paul # *U FBrf0188685 *a Duperchy *b E. *t 2005.9.8 *T personal communication to FlyBase *u Helping FlyBase: EDRC-CB17. *F Date: Tue, 6 Sep 2005 10:32:45 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-CB17 *F To: esther.duperchy@zoo.ox.ac.uk *F Cc: rd120@gen.cam.ac.uk *F Dear Esther, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Investigating the essential role of the phosphatase PP1beta-MYPT-75D in *F Drosophila non-muscle myosin regulation. *F You mention a gene symbol that is new to FlyBase, MYPT-75D. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F From: Esther Duperchy *F Date: Thu, 8 Sep 2005 11:10:59 \+0100 (BST) *F To: 'Rachel Drysdale (Genetics)' *F Subject: Re: Helping FlyBase: EDRC-CB17 *F Dear Rachel, *F MYPT-75D, as we called it, corresponds to CG6896. See: *F Vereshchagina N, Bennett D, Szoor B, Kirchner J, Gross S, Vissi E, White-Cooper *F H, Alphey L. The essential role of PP1beta in Drosophila is to regulate *F nonmuscle myosin. *F Mol Biol Cell. 2004 Oct;15(10):4395-405. Epub 2004 Jul 21. *F PMID: 15269282 PubMed \- in process *F Do not hesitate to contact me for further informations. *F Best Regards, *F Esther # *U FBrf0188686 *a Bouge *b A.L. *t 2005.9.8 *T personal communication to FlyBase *u Helping FlyBase: EDRC-OG12. *F Date: Tue, 6 Sep 2005 10:37:46 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-OG12 *F To: anne-laure.bouge@u-bourgogne.fr *F Cc: rd120@gen.cam.ac.uk *F Dear Anne-Laure, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Tenectin, a new ligand of alphaPS2 betaPS integrin. *F You mention a gene symbol that is new to FlyBase, tenectin. Do you *F know which of the Genome Project CG annotations your gene corresponds *F to? All the CGs have corresponding gene records in FlyBase already and *F we don't like to make duplicate records for what is actually the same *F gene unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F If you have settled on a short symbol for tenectin, now would be a good *F time to tell me, so that it can go into FlyBase correctly, from the *F outset. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Thu, 8 Sep 2005 14:57:36 \+0200 *F To: 'Rachel Drysdale (Genetics)' *F From: anne-laure BOUGE *F Subject: Re: Helping FlyBase: EDRC-OG12 *F \-- *F Dear Rachel, *F tenectin (Tnc) corresponds to the CG 13648. *F best regards, *F AL.B *F UMR CNRS 5548 *F 'Développement Communication Chimique' *F 6, Bd Gabriel *F 21000 Dijon, France # *U FBrf0188687 *a Russell *b S. *t 2005.9.6 *T personal communication to FlyBase *u P{GAL4-sim.3.7} insertions. *F Date: Tue, 06 Sep 2005 09:54:41 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GAL4-sim.3.7} insertions *F Mime-Version: 1.0 *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Steve Russell, University of Cambridge. *F P{GAL4-sim.3.7}2 is a second chromosome insertion. *F P{GAL4-sim.3.7}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188688 *a Silies *b M. *t 2005.9.9 *T personal communication to FlyBase *u Helping FlyBase: EDRC-NB16. *F Date: Tue, 6 Sep 2005 10:29:29 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-NB16 *F To: msilies@uni-muenster.de *F Cc: rd120@gen.cam.ac.uk *F Dear Marion, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Wex is required for glial cell migration in the Drosophila PNS *F You mention a gene symbol that is new to FlyBase, wex. Do you know which of the *F Genome Project CG annotations your gene corresponds to? All the CGs *F have corresponding gene records in FlyBase already and we don't like to *F make duplicate records for what is actually the same gene unless we *F can't avoid it. The CG symbols become synonyms when an annotation is *F named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Fri, 09 Sep 2005 15:33:25 \+0200 (MES) *F From: Marion Silies *F Subject: Re: Helping FlyBase: EDRC-NB16 *F To: 'Rachel Drysdale (Genetics)' *F Dear Rachel, *F wex corresponds to CG3000 (rap = retina aberrant in pattern). *F sorry for the confusion, *F best regards *F marion # *U FBrf0188689 *a Carlson *b J. *t 2005.9.12 *T personal communication to FlyBase *u FlyTag EST project. *F File deposited: Carlson.2005.9.12-1.doc ; File date: 2005.9.12 ; File size: *F 181248 ; File format: doc # *U FBrf0188690 *a Claussen *b M. *t 2005.9.14 *T personal communication to FlyBase *u Helping FlyBase: EDRC-CB14. *F Date: Tue, 6 Sep 2005 10:33:09 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: Helping FlyBase: EDRC-CB14 *F To: maike.claussen@izb.unibe.ch *F Cc: rd120@gen.cam.ac.uk *F Dear Maike, *F We are currently curating the abstracts for the 19th (Eger) *F European Drosophila Research Conference, for FlyBase. *F I am writing in connection with your abstract: *F Analysis of Drosophila Translin and Trax function *F You mention a gene symbol that is new to FlyBase, Trax. Do you know *F which of the Genome Project CG annotations your gene corresponds to? *F All the CGs have corresponding gene records in FlyBase already and we *F don't like to make duplicate records for what is actually the same gene *F unless we can't avoid it. The CG symbols become synonyms when an *F annotation is named with a more descriptive or functional name. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Wed, 14 Sep 2005 12:29:09 \+0200 *F From: Maike *F Subject: Re: Helping FlyBase: EDRC-CB14 *F To: 'Rachel Drysdale (Genetics)' *F Hi Rachel, *F Trax corresponds to CG5063. It shares homologies with human and mouse *F Translin associated factor X (TRAX). *F Regards, *F Maike # *U FBrf0188691 *a Verstreken *b P. *t 2005.7.11 *T personal communication to FlyBase *u Cask/Caki. *F Subject: FW: Cask/Caki *F Date: 7/11/2005 *F From: 'Verstreken, Patrik' *F To: *F Cc: 'Bellen, Hugo J' *F Dear Rachel, *F When we did our original BLAST searches we used rat CASK (gb: Q62915) as well *F as mouse CASK-A (gb: CAA76646) and CASK-B (gb: CAA76647). Our searches back *F then showed alignment with 2 Celera scaffold sequences (note that the genome *F sequence was not yet assembled when we did our BLAST searches). One *F of these Celera sequences mapped to BAC 42I20, which was cytologically mapped *F to 93F, the other mapped to BAC 04F03 which was mapped to 42D-E. *F We have now repeated the t-BLAST-n searches using the finished and assembled *F genome sequence, and find that all mammalian CASK genes reveal only 1 *F homologue in flies: CAKI, which maps to 93F (as is annotated in Flybase). We *F also re-checked the Celera genomic scaffolds that we identified to contain *F CASK homologues. Post genome assembly, we now find that both these scaffold *F map to 93F, further demonstrating that flies contain only 1 CASK homologue. *F hope this helps. *F Patrik # *U FBrf0188692 *a Davis *b R. *t 2005.9.29 *T personal communication to FlyBase *u P{UAS-CrebB-17A-a.cor} insertions. *F Date: Thu, 29 Sep 2005 16:24:04 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-CrebB-17A-a.cor} insertions *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Ron Davis, Baylor College of Medicine (8/05). *F P{UAS-CrebB-17A-a.cor}T7.1 and P{UAS-CrebB-17A-a.cor}T25.4 are *F homozygous viable and fertile, third chromosome insertions. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188693 *a Dura *b J.M. *t 2005.9.29 *T personal communication to FlyBase *u P{UAS-Alh.LZ1}3. *F Date: Thu, 29 Sep 2005 14:17:48 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-Alh.LZ1}3 *F The following information was provided to the Bloomington Stock *F Center by Jean-Maurice Dura, Institut de Genetique Humaine, CNRS *F Montpellier (8/05). *F P{UAS-Alh.LZ1}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188694 *a Brenman *b J. *t 2005.8 *T personal communication to FlyBase *u P{UAS-seq.B} construct and insertion. *F Date: Thu, 29 Sep 2005 13:45:56 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-seq.B} construct and insertion *F The following information accompanied stocks donated to the *F Bloomington Drosophila Stock Center by Jay Brenman, University of *F North Carolina School of Medicine (8/05). *F P{UAS-seq.B} contains the wild type sequoia gene driven by UAS. *F P{UAS-seq.B}3 is a homozygous viable and fertile, third chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188695 *a Thomas *b B. *c J. *d Merriam *t 2005.9.29 *T personal communication to FlyBase *u P{A92}LG51 and P{A92}rhoBB02. *F Date: Thu, 29 Sep 2005 12:15:35 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{A92}LG51 and P{A92}rhoBB02 *F The following information was provided to the Bloomington Stock *F Center by Barbara Thomas, NIH, and John Merriam, UCLA. *F P{A92}LG51 and P{A92}rhoBB02 were isolated in the lab of John *F Merriam. The enhancer trap expression of lacZ in both lines is *F specific to the R8 eye cell. The expression of P{A92}rhoBB02 *F begins slightly earlier in development than the expression of *F P{A92}LG51. P{A92}LG51 is a third chromosome insertion. *F P{A92}rhoBB02 currently appears in FlyBase entry FBti0009169 as *F P{lacZ}rhoBB02. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188696 *a O'Kane *b C. *t 2005.8 *T personal communication to FlyBase *u P{GawB}OK10. *F Date: Sun, 02 Oct 2005 15:00:59 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GawB}OK10 *F The following information accompanied stocks donated to the *F Bloomington Stock Center by Cahir O'Kane, Cambridge University (8/05). *F P{GawB}OK10 is a homozygous viable and fertile, third chromosome *F insertion currently listed in FlyBase as P{GAL4)OK10 (FBti0004215). *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188697 *a Pardue *b M.L. *t 2005.10.5 *T personal communication to FlyBase *u XL telomere HeT element corrections. *F Date: Wed, 5 Oct 2005 12:41:05 \-0400 (EDT) *F From: Madeline Crosby *F Subject: Re: pc for Mary-Lou's XL telomere HeT element corrections *F To: Camcur@morgan.harvard.edu, crosby@morgan.harvard.edu, rd120@gen.cam.ac.uk *F Cc: crosby@morgan.harvard.edu *F XL telomere HeT-A element corrections submitted by Mary-Lou Pardue *F *F 1--2293 Het 1 *F 2294--2331 Het 2 *F 2332--4692 Het 3 *F 4693--4727 Het 4 *F 4728--4771 Het 5 *F 4772--10777 Het 6 *F 10799-11024 unidentified sequence 23aa identity to a 35 aa cDNA *F 11024--11097 Het 7 *F Confirmed following new insertions and updates to current insertion records: *F current symbol orig. extent syn. new extent *F FBti0062856 HeT-A{}4795 X: 1..2293 Het 1 No chg *F FBti0063564 HeT-A{}5503 X: 2295..2329 Het 2 X: 2294..2331 *F FBti0063565 HeT-A{}5504 X: 2330..4771 Het 3 X: 2332..4692 *F FBti0063566 HeT-A{}5505 X: 3335..4690 remove (merge) *F new HeT-A{}6253 Het 4 X: 4693-4727 *F new HeT-A{}6254 Het 5 X: 4728-4771 *F FBti0062861 HeT-A{}4800 X: 4821..10778 Het 6 X: 4772-10777 *F FBti0062863 HeT-A{}4802 X: 11017..11081 Het 7 X: 11024-11097 *F Merge: FBti0063566 becomes synonym for FBti0063565. # *U FBrf0188698 *a Delidakis *b C. *c F. *d Schweisguth *e E. *f Lai *g S. *h Bray *t 2005.10.10 *T personal communication to FlyBase *u mind-bomb gene symbol. *F Date: Thu, 6 Oct 2005 13:44:57 \+0100 (BST) *F From: 'Rachel Drysdale (Genetics)' *F Subject: mind-bomb gene symbol *F To: schweisg_AT_wotan.ens.fr, delidaki_AT_imbb.forth.gr, *F sjb32_AT_mole.bio.cam.ac.uk, gerry_AT_fruitfly.org *F Cc: rd120_AT_gen.cam.ac.uk *F Dear Sarah, Christos, Gerry, and Francois, *F The mind-bomb gene (FBgn0036558) is in FlyBase at the moment with both *F a symbol and full name of 'mind-bomb'. This is not terribly *F satisfactory \- it ought to have a short symbol. In papers and *F abstracts it has been referred to as D-mib, and (in this year's EDRC *F abstract book) as Mib1. Valid symbols are not prefixed with D for *F Drosophila, and mib is already in existence as a symbol for FBgn0002744 *F ('miniature bristles') so we (FB) haven't been able to use mib as the symbol. *F Mib1 as a symbol is unique \- though it has only this year appeared in *F an abstract. Gene symbols should only be capitalised when they are *F named for a product or molecular characteristic and I have a feeling *F that the 'mind-bomb' name comes from a phenotypic characteristic *F (correct me if I'm wrong here). *F mib1 is also available, so perhaps the best solution would be to change *F the symbol from 'mind-bomb' to 'mib1' and full name from 'mind-bomb' to *F 'mind bomb 1'. That seems lucid, do you agree? *F If not, is there a better solution that everyone might be in favor of? *F It is unusual to change symbols/names in FB, and we only do it if there *F is agreement by everyone working on the gene at the time \- so it is *F with some trepidation I start the debate, but it is time that the gene *F got a decent symbol \- I think we'd all agree on that. *F With best regards, *F Rachel. *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Rachel Drysdale. *F FlyBase (Cambridge), *F Department of Genetics, *F University of Cambridge, *F Downing Street, email: rd120@gen.cam.ac.uk *F Cambridge, CB2 3EH, Ph : 01223-333963 *F UK. FAX: 01223-333992 *F FlyBase: http://flybase.org/ *F ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ *F Date: Thu, 06 Oct 2005 18:05:57 \+0300 *F To: 'Rachel Drysdale (Genetics)' , schweisg@wotan.ens.fr, *F sjb32@mole.bio.cam.ac.uk, gerry@fruitfly.org *F From: Christos Delidakis *F Subject: Re: mind-bomb gene symbol *F Cc: pitsouli@imbb.forth.gr *F Dear Rachel, Francois, Sarah and Gerry *F I agree with the name mib1 \- especially since there exists a mind bomb *F paralogue (CG17492), which could accordingly be named mib2. Alternatively, *F similar mammalian genes are named mind bomb and skeletrophin, respectively *F (by BLASTP on mouse proteins), so we could use the names mind bomb1 and *F skeletrophin for the Drosophila genes. I tend to favour the simpler mib1, *F mib2 terminology, which is directly indicative of their structural similarity. *F Best regards *F Christos *F ~~~~~~~~~~~~~~~~~~~~~~~~ *F Christos Delidakis *F Institute of Molecular Biology and Biotechnology, FoRTH *F Vassilika Vouton *F GR-71110, Heraklion, Greece *F tel: \+30-2810-391-112 *F fax: \+30-2810-391-104 *F Subject: Re: mind-bomb gene symbol *F From: schweisg@biologie.ens.fr *F To: 'Rachel Drysdale (Genetics)' *F Cc: sjb32_AT_mole.bio.cam.ac.uk, gerry_AT_fruitfly.org, laie_AT_mskcc.org, *F delidaki_AT_imbb.forth.gr, pitsouli_AT_imbb.forth.gr *F Dear Rachel, *F I agree with your suggestion to name the mind-bomb gene (FBgn0036558): *F 'mind bomb 1'/'mib1' (full name/abbreviation). This nomenclature would *F particularly make sense if we could also have CG17492 named: 'mind bomb *F 2'/'mib2' as suggested by Christos. Can we decide on this also now? *F Best, *F Francois *F To: schweisg@biologie.ens.fr *F cc: 'Rachel Drysdale (Genetics)' , *F sjb32_AT_mole.bio.cam.ac.uk, gerry_AT_fruitfly.org, delidaki_AT_imbb.forth.gr, *F pitsouli_AT_imbb.forth.gr *F From: 'Eric Lai' *F Subject: Re: mind-bomb gene symbol *F Date: Sat, 8 Oct 2005 22:07:35 \-0400 *F I agree with renaming 'mind bomb 1'/'mib1' and 'mind bomb2'/'mib2'. *F regards, *F eric *F Eric Lai *F Assistant Member, Sloan-Kettering Institute *F 521 Rockefeller Research Labs *F 1275 York Avenue, Box 252 *F New York, NY 10021 *F ph: 212-639-5578 *F fax: 212 717-3604 *F site: http://www.mskcc.org/mskcc/html/52949.cfm *F Date: Mon, 10 Oct 2005 10:42:56 \+0100 *F To: 'Rachel Drysdale (Genetics)' *F From: Sarah Bray *F Subject: Re: mind-bomb gene symbol *F No objections, suggestions are fine with me, *F S. # *U FBrf0188699 *a Brenman *b J. *t 2005.8 *T personal communication to FlyBase *u P{UAS-nod.GFP} insertions. *F Date: Thu, 06 Oct 2005 12:52:29 \-0500 *F To: rd120@gen.cam.ac.uk *F From: Kevin Cook *F Subject: P{UAS-nod.GFP} insertions *F The following information accompanied stocks donated to the *F Bloomington Drosophila Stock Center by Jay Brenman, University of *F North Carolina School of Medicine (8/05). *F P{UAS-nod.GFP}2 is a homozygous viable and fertile, second chromosome *F insertion. *F P{UAS-nod.GFP}3 is a homozygous viable and fertile, third chromosome *F insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188700 *a Miller *b K. *c D. *d Van Vactor *t 2005.9 *T personal communication to FlyBase *u P{UAS-n-syb-GFP.E}3 & P{GawB}D42. *F Date: Wed, 05 Oct 2005 13:16:03 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{UAS-n-syb-GFP.E}3 & P{GawB}D42 *F The following information accompanied a stock donated to the *F Bloomington Stock Center by Kyle Miller and Davie Van Vactor, Harvard *F Medical School (9/05). *F P{UAS-n-syb-GFP.E}3 is a third chromosome insertion. It may be the *F same as P{UAS-n-syb-GFP.E}PSE1 (FBti0015928). *F P{GawB}D42 is expressed selectively in motor neurons in 3rd instar larvae. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188701 *a Bloomington Drosophila Stock Center *b ?. *t 2005.10.7 *T personal communication to FlyBase *u Phenotype of EP984. *F Date: Fri, 7 Oct 2005 15:23:58 \-0500 (EST) *F From: Kathy Matthews *F Subject: phenotype of EP984 *F To: flybase-updates@morgan.harvard.edu *F Observation from the Bloomington Drosophila Stock Center \-- *F P{EP}EP984 is an insertion in CG31005, and therefore creates *F allele CG31005EP984. This alleles appears to have a *F dominant neurological or motor defect, although we have not confirmed *F that the phenotype observed in the balanced stock is caused *F by the insertion allele. CG31005EP984/TM6B adults have *F twitchy movements and they are flightless. # *U FBrf0188702 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC126. *F Date: Thu, 27 Oct 2005 11:26:40 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC126 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3L)BSC126 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC126 was isolated as a FLP recombinase-induced recombination *F event involving P{XP}d04808 and PBac{WH}CG32343f06767. The *F deletion was isolated as a chromosome lacking miniwhite markers in *F progeny of w1118, P{hsFLP}1; P{XP}d04808/ PBac{WH}CG32343f06767 *F females crossed to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 *F males. The females were heat shocked as larvae as described in Parks *F et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross *F and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks *F (Thibault et al., Nature Genetics 36: 283-287, 2004; *F FBrf0175002). The recombination event generated the genetic element *F P+PBac{XP5.WH5}BSC126 from the segment of P{XP}d04808 to the left of *F its FRT site and the segment of and PBac{WH}CG32343f06767 to the *F right of its FRT site. Its presence was verified using the PCR *F methods and primers described in Parks et al. The cytological *F breakpoints of Df(3L)BSC126 predicted from the transposable element *F insertions sites are 61B3;61C1. It failed to complement Df(3L)Exel6084. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188703 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC127. *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC127 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3L)BSC127 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC127 was isolated as a FLP recombinase-induced recombination *F event involving PBac{WH}drprf04411 and P{XP}d06594. The deletion *F was isolated as a chromosome lacking miniwhite markers in progeny of *F w1118, P{hsFLP}1; PBac{WH}drprf04411/P{XP}d06594 females crossed *F to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females *F were heat shocked as larvae as described in Parks et al., Nature *F Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in *F preceding and succeeding generations maintained the original genetic *F background of the Exelixis insertion stocks (Thibault et al., Nature *F Genetics 36: 283-287, 2004; FBrf0175002). The recombination event *F generated the genetic element P+PBac{XP5.WH5}BSC127 from the segment *F of PBac{WH}drprf04411 to the left of its FRT site and the segment *F of P{XP}d06594 to the right of its FRT site. Its presence was *F verified using the PCR methods and primers described in Parks et *F al. Exelixis, Inc. determined the insertion site of P{XP}d06594 to *F be at Release 3 genomic coordinate 1774835 on arm 3L. The Gene *F Disruption project determined the insertion site of P{XP}d06594 to *F be at Release 3 genomic coordinate 1774992 on arm 3L. The cytological *F breakpoints of Df(3L)BSC127 predicted from the transposable element *F insertions sites are 62B1;62B4. It failed to complement dlt04276, *F Df(3L)Aprt-1 and Df(3L)Exel6088. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188704 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC12. *F Date: Thu, 27 Oct 2005 11:26:44 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC128 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3L)BSC128 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC128 was isolated as a FLP recombinase-induced recombination *F event involving PBac{RB}Mtche00205 and P{XP}thoc7d05792. The *F deletion was isolated as a chromosome lacking miniwhite markers in *F progeny of w1118, P{hsFLP}1; *F PBac{RB}Mtche00205/P{XP}thoc7d05792 females crossed to w1118; *F wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females were heat *F shocked as larvae as described in Parks et al., Nature Genetics 36: *F 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and *F succeeding generations maintained the original genetic background of *F the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: *F 283-287, 2004; FBrf0175002). The recombination event generated the *F genetic element P+PBac{XP5.RB3}BSC128 from the segment of *F PBac{RB}Mtche00205 to the left of its FRT site and the segment of *F P{XP}thoc7d05792 to the right of its FRT site. Its presence was *F verified using the PCR methods and primers described in Parks et al *F with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' *F for the RB3' minus primer in the Hybrid PCR protocol in the *F Supplementary Methods. The cytological breakpoints of Df(3L)BSC128 *F predicted from the transposable element insertions sites are *F 61B2;61B2. It failed to complement Df(3L)Exel6084. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188705 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC129. *F Date: Thu, 27 Oct 2005 11:26:49 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC129 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3L)BSC129 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC129 was isolated as a FLP recombinase-induced recombination *F event involving P{XP}d01788 and PBac{WH}CG12077f05249. The *F deletion was isolated as a chromosome lacking miniwhite markers in *F progeny of w1118, P{hsFLP}1; P{XP}d01788/ PBac{WH}CG12077f05249 *F females crossed to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 *F males. The females were heat shocked as larvae as described in Parks *F et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross *F and crosses in preceding and succeeding generations maintained the *F original genetic background of the Exelixis insertion stocks *F (Thibault et al., Nature Genetics 36: 283-287, 2004; *F FBrf0175002). The recombination event generated the genetic element *F P+PBac{XP5.WH5}BSC129 from the segment of P{XP}d01788 to the left of *F its FRT site and the segment of PBac{WH}CG12077f05249 to the right *F of its FRT site. Its presence was verified using the PCR methods and *F primers described in Parks et al. Exelixis, Inc. determined the *F insertion site of P{XP}d01788 to be at Release 3 genomic coordinate *F 3227264 on arm 3L. The cytological breakpoints of Df(3L)BSC129 *F predicted from the transposable element insertions sites are *F 63C1;63D1. It failed to complement Df(3L)1227, Df(3L)HR232 and Df(3L)HR119. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188706 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC130. *F Date: Thu, 27 Oct 2005 11:26:52 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC130 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3L)BSC130 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC130 was isolated as a FLP recombinase-induced recombination *F event involving PBac{WH}CG5660f01783 and P{XP}bold01760. The *F deletion was isolated as a chromosome lacking miniwhite markers in *F progeny of w1118, P{hsFLP}1; PBac{WH}CG5660f01783/ *F P{XP}bold01760 females crossed to w1118; wgSp-1/CyO; *F sensLy-1/TM6B, Tb1 males. The females were heat shocked as *F larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 *F (FBrf0175003). This cross and crosses in preceding and succeeding *F generations maintained the original genetic background of the *F Exelixis insertion stocks (Thibault et al., Nature Genetics 36: *F 283-287, 2004; FBrf0175002). The recombination event generated the *F genetic element P+PBac{XP5.WH5}BSC130 from the segment of *F PBac{WH}CG5660f01783 to the left of its FRT site and the segment of *F P{XP}bold01760 to the right of its FRT site. Its presence was *F verified using the PCR methods and primers described in Parks et *F al. Polytene chromosome squashes of Df(3L)BSC130 showed the *F breakpoints 66E5-F1;66F5-67A1. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188707 *a Christensen *b S. *c K. *d Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(2R)BSC131. *F Date: Thu, 27 Oct 2005 11:26:54 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(2R)BSC131 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(2R)BSC131 *F Stacey Christensen and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(2R)BSC131 was isolated as a FLP recombinase-induced recombination *F event involving P{XP}Mmp2d11288 and PBac{RB}e02075. The deletion *F was isolated as a chromosome lacking miniwhite markers in progeny of *F P{hsFLP}1, w1118; P{XP}Mmp2d11288/PBac{RB}e02075 males crossed to *F w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat *F shocked as larvae as described in Parks et al., Nature Genetics 36: *F 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and *F succeeding generations maintained the original genetic background of *F the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: *F 283-287, 2004; FBrf0175002). The recombination event generated the *F genetic element P+PBac{XP5.RB3}BSC131 from the segment of *F P{XP}Mmp2d11288 to the left of its FRT site and the segment of *F PBac{RB}e02075 to the right of its FRT site. Its presence was *F verified using the PCR methods and primers described in Parks et al *F with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' *F for the RB3' plus primer in the Hybrid PCR protocol in the *F Supplementary Methods. The cytological breakpoints of Df(3L)BSC130 *F predicted from the transposable element insertions sites are *F 46A1;46B4. It failed to complement dap4. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188708 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3L)BSC125. *F Date: Thu, 27 Oct 2005 11:26:38 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3L)BSC125 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3L)BSC125 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3L)BSC125 was isolated as a FLP recombinase-induced recombination *F event involving PBac{WH}f04555 and P{XP}d10863. The deletion was *F isolated as a chromosome lacking miniwhite markers in progeny of *F w1118, P{hsFLP}1; PBac{WH}f04555/PBac{XP}d10863 females crossed to *F w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females *F were heat shocked as larvae as described in Parks et al., Nature *F Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in *F preceding and succeeding generations maintained the original genetic *F background of the Exelixis insertion stocks (Thibault et al., Nature *F Genetics 36: 283-287, 2004; FBrf0175002). The recombination event *F generated the genetic element P+PBac{XP5.WH5}BSC125 from the segment *F of PBac{WH}f04555 to the left of its FRT site and the segment *F of P{XP}d10863 to the right of its FRT site. Its presence was *F verified using the PCR methods and primers described in Parks et *F al. The cytological breakpoints of Df(3L)BSC125 predicted from the *F transposable element insertions sites are 61B3;61B3. It failed to *F complement Df(3L)Exel6084. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188709 *a Andrade *b R. *c J. *d Gresens *e M. *f Deal *g K. *h Cook *t 2005.10.27 *T personal communication to FlyBase *u Isolation and characterization of Df(3R)BSC124. *F Date: Thu, 27 Oct 2005 11:26:35 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: Isolation and characterization of Df(3R)BSC124 *F Cc: Stacey Christensen , mdeal_at_indiana.edu, Jill *F Gresens , Rachel Andrade *F , kaufman_at_bio.indiana.edu *F Isolation and characterization of Df(3R)BSC124 *F Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook *F Bloomington Stock Center *F Indiana University *F Df(3R)BSC124 was isolated as a FLP recombinase-induced recombination *F event involving PBac{WH}f01767 and P{XP}CG17838d09234. The *F deletion was isolated as a chromosome lacking miniwhite markers in *F progeny of w1118, P{hsFLP}1; PBac{WH}f01767/P{XP}CG17838d09234 *F males crossed to w1118; P{hs-hid}3, Dr1/TM6B females. The males *F were heat shocked as larvae as described in Parks et al., Nature *F Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in *F preceding and succeeding generations maintained the original genetic *F background of the Exelixis insertion stocks (Thibault et al., Nature *F Genetics 36: 283-287, 2004; FBrf0175002). The recombination event *F generated the genetic element P+PBac{XP5.WH5}BSC124 from the segment *F of PBac{WH}f01767 to the left of its FRT site and the segment of *F P{XP}CG17838d09234 to the right of its FRT site. Its presence was *F verified using the PCR methods and primers described in Parks et *F al. The cytological breakpoints of Df(3L)BSC124 predicted from the *F transposable element insertions sites are 92F10;92F12-93A1. It *F failed to complement cdc2c3 and P{PZ}CG1783803806. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188710 *a Levis *b R. *t 2005.10.14 *T personal communication to FlyBase *u piggyBac insertion f07705. *F Date: Fri, 14 Oct 2005 19:28:03 \-0400 *F To: flybase-help@morgan.harvard.edu *F From: Robert Levis *F Subject: piggyBac insertion f07705 *F Cc: 'Kathy Matthews' *F The piggyBac insertion f07705 (FBti0042690) was generated by Exelixis *F Corp and reported to be inserted in the vicinity of the 5' end of *F CG5003 on 3R. It was selected by Allan Spradling to be sent to the *F Bloomington Stock Center and is on their current stock list (# *F 19097). It is annotated by FlyBase as an allele of CG5003. Joe *F Carlson at LBNL reanalyzed all of the Exelixis sequence traces for *F their collection of piggyBac's and P-elements and submitted the *F flanks to GenBank that were above a minimum length and quality. He *F did not submit any flanks for f07705, apparently because of the poor *F sequence quality. I was curious about this insertion and BLASTed the *F poor quality flanking sequences that I got from Joe's database. *F The 5' flanking sequence is: *F ttaaaggtatgttg *F ggtaatattagaggaagATGCATCTCTTCGTAGATATGAATATTCCTAGT *F AACAGTTTATCTCGGGATC *F reading out from the piggyBac. When I BLAST this to Release 4.1 *F genomic sequence, the best alignment is a 40/49 match, beginning at *F base 35, to a segment beginning at 3R: 23742286. This is 37 bp *F uptream of CG5003. While this is the best alignment, it is not very *F convincing. *F The 3' flanking sequence is: *F TTAATGCAACTGAAGTCCAAACTGCATACCACACACTCCTCCATAAGATT *F GCCGCCATCCTAGGTATTTTTGTGCGC *F reading out from the piggBac. When I BLAST this to Release 4.1 *F genomic sequence, the best alignment is a 71/77 match, beginning at *F base 1, to a segment beginning at 2L: 13827718, which is within the *F tam gene. *F Taken together, these data suggest that this was a double insert line *F at the time the flanking sequences were determined by Exelixis. *F Perhaps this message should be made into a personal communication and *F added as a reference to the record for the insertion and fly stock. *F ...Bob Levis *F \-- *F \------------------------------------ *F Robert W. Levis, Ph.D. *F Carnegie Institution of Washington *F Department of Embryology *F 3520 San Martin Drive *F Baltimore, MD 21218, U.S.A. *F phone: 1-(410) 246-3038 *F fax: 1-(410) 243-6311 *F email: levis@ciwemb.edu *F \------------------------------------ # *U FBrf0188711 *a Garrity *b P. *t 2005.11.2 *T personal communication to FlyBase *u P{GMR-GFP.P}2. *F Date: Wed, 02 Nov 2005 16:24:20 \-0500 *F To: flybase-updates@morgan.harvard.edu *F From: Kevin Cook *F Subject: P{GMR-GFP.P}2 *F The following infomation accompanied stocks donated to the *F Bloomington Stock Center by Paul Garrity, *F Massachusetts Institute of Technology (10/05). *F P{GMR-GFP.P}2 is a second chromosome insertion. *F __________________________________________________________ *F Kevin Cook, Ph.D. Bloomington Drosophila Stock Center *F Department of Biology http://flystocks.bio.indiana.edu *F Jordan Hall 142 *F Indiana University 812-856-1213 *F 1001 E. Third St. 812-855-2577 (fax) *F Bloomington, IN 47405-7005 kcook@bio.indiana.edu # *U FBrf0188730 *a Wasser *b M. *t 2005.6.16 *T personal communication to FlyBase *u *F From: 'Martin Wasser' *F To: 'Ruth Seal' *F Subject: Re: east alleles *F Date: Thu, 16 Jun 2005 12:06:28 \+0800 *F Dear Ruth, *F The two east alleles P-86 and hop-2 were generated in Prof. Veronica *F Rodrigues' lab by excision of the P-element of the line eastPry+ETX3 *F described in VijayRaghavan et al. (1992). P-86 is a precise excision that *F restores normal behavior while hop-2 is an imprecise excision that results *F in lethality. I have checked all publications and believe that the 2 alleles *F have not been referenced properly. I also don't think that different allele *F names have been used. I hope this information is of some help to you. *F Best Regards *F Martin *F \----- Original Message \----- *F From: 'Ruth Seal' *F To: *F Cc: *F Sent: Tuesday, June 14, 2005 5:00 PM *F Subject: east alleles *F > Dear Dr Wasser *F > *F > I am a curator at FlyBase and and I have been curating your publication *F > The *F > Drosophila EAST protein associates with a nuclear remnant during mitosis *F > and *F > constrains chromosome mobility.' Wasser and Chia, 2003, J. Cell Sci. *F > 116(9): *F > 1733--1743. I hope you can help me out so that I can *F > curate your paper as accurately as possible. *F > *F > I was unable to identify two east alleles from your paper, P-86 and hop-2. *F > You *F > give two references for papers that describe the east alleles used: *F > *F > Wasser and Chia, 2000, Nature Cell Biol. 2(5): 268--275 *F > VijayRaghavan et al., 1992, Dev. Biol. 154: 23--36 *F > *F > I have identified alleles from the first reference so I am wondering if *F > P-86 and *F > hop-2 might be described in the second reference, but with slightly *F > different *F > names? *F > *F > Thankyou, *F > Ruth *F > # *U FBrf0188731 *a Bartolome *b C. *t 2005.7.27 *T personal communication to FlyBase *u jockey insertion. *F From: FB_Auto_Mailer@rail.bio.indiana.edu *F Date: Wed, 27 Jul 2005 09:38:02 \-0500 (EST) *F To: flybase-help@morgan.harvard.edu *F Subject: FB Help Mail: 066 jockey insertion *F comments: The jockey insertion 6115 located at 4:731601-732486 (second *F intron of ey gene) was previously reported in D. melanogaster and D. simulans *F by C. Bartolome and X. Maside: The lack of recombination drives the fixation *F of transposable elements on the fourth chromosome of Drosophila melanogaster. *F Genet. Res. 83 (2), 91-100 (2004) *F The sequences have been deposited in GenBank with Accession numbers: *F DQ091189 and DQ091188 *F realname: C. Bartolome *F reply-to: cbhusson@usc.es # *U FBrf0188732 *a Grosshans *b J. *t 2005.8.11 *T personal communication to FlyBase *u *F From: Jörg Großhans *F Date: Thu, 11 Aug 2005 14:18:55 \+0200 *F To: Ruth Seal *F Subject: Re: Df(3L)XG9 *F All Df(3L)XG chromosomes are X-ray induced white revertants of P(lacW)B204, *F including XG9. Kevin Cook from Bloomington determined the breakpoints of all *F XG deficiencies but XG9. The only breakpoint information that I have is: *F distal breakpoint: *F between STS71-3 and STSDm2216 *F position of STS Dm2216: nt45452..45126 in AE003530 between CG13453 *F and CrebA *F position of STS71-3: nt 37440..37606 in AE003530, within CG17014 *F proximal breakpoint *F not determined (beyond the STS that I tested) *F breakpoint of XG7 *F proximal: *F between STS71-3 and STSDm2216 #