There are 7 sets of S.E.M. pictures in this update of the FlyBase image files. All are saved in a Jpeg format and are 640x480 pixels in size. In the case of the normal embryos we have provided both a labelled and unlabelled set. The images are those used to make the movies of embryogenesis which will be described below. Each set of images is numbered sequentially from youngest to oldest generally starting at blastoderm. The image sets are as follows: 1)Adult heads frontal view. These show the affects of the Antp and pb homeotic mutations on antennal and mouthpart morphology respectively. 2)Gastrulation/Dorsal View labeled and unlabeled. There is a total of 18 images starting at blastoderm and ending with the completion of dorsal closure and cuticle deposition. 3)Gastrulation/Lateral View labeled and unlabeled. There is a total of 12 images starting at blastoderm and ending with segmentation and germ band shortening. 4)Head involution ventral view. There is a total of 19 images starting with the point at which the gnathal segments are formed and ending with the involution of these segments into the stomadeum. It should be noted that the final image does not correspond to the end of head involution as it is not possible to get comparable S.E.M.'s of this part of the embryo after cuticle deposition is complete. 5)Ventral view of embryo during mesoderm invagination. There is a total of 6 images starting at the onset of the formation of the ventral furrow and ending just prior to the completion of mesoderm invagination. 6)BCD mutant animals dorsal view. There is a total of 23 images starting at early gastrula and ending with dorsal closure and cuticle deposition. 7)FTZ mutant animals lateral view. There is a total of 25 images starting at blastoderm and ending with dorsal closure and cuticle deposition. All images were obtained by Dr. R. Turner in the Department of Biology, Indiana University Bloomington, IN. As noted above the image collections were used to make 'movies' of embryogenesis. The still images were morphed together using commercially available software packages and Power MacIntosh hardware. Both Morph and Elastic Reality were used with roughly comparable results. The 'Morphing' was done by Jeff Giacoletti and Chris Macri. The movies are 240x180 jpeg compressed and play at a rate of 15 frames per second. They should be viewable using standard movie play applications. The movies are: 1)Gastrulation lateral view 2)Gastrulation Ventral view 3)Gastrulation Dorsal view 4)Head involution movie 5)FTZ mutation movie 6)BCD mutation movie 7)Credits movie In addition to these morphed versions of embryogenesis using real animals as images we have made a morphed cartoon version of embryogenesis using images from The Atlas of Drosophila Development by Volker Hartenstein, Cold Spring Harbor Laboratory Press (1993). This movie can be found in the Animation folder along with the scanned individual images used to make the movie. FlyBase is starting a compilation of anitomical drawings and other images which will be used as reference marterial for the description of gene expression data. Our initial effort in this direction has involved a collaboration with Dr. Peter Bryant at The Developmental Biology Center, University of California, Irvine, CA 92717. Dr. Bryant has supplied FlyBase with copies of drawings of adult body parts and these have been scanned to make them available in digital form. All the images were captured at 300 dpi and are in jpeg format. The drawings of adult structures have been organised by anatomical region: Abdomen, Genetalia, Head and Thorax. In the future we will augment these images with pictures of imaginal discs and their fate maps as well as S.E.M. photographs of the adult body parts represented in the drawings. The images in the "P. Bryant Drawings" folder are used with permission of: Academic Press, Inc 525 B Street, Suite 1900 San Diego, California 92101-4495 tel 619-699-6412 fax 619-699-6715 Flybase is indebted to Jeff Giacoletti, Chris Macri, Rudi Turner, Michael Kaufmann and Peter Bryant for their contributions to the images portion of the FlyBase data set.